Essential oils produced by in vitro shoots of sage (Salvia officinalis L.).
In vitro shoots of sage (Salvia officinalis L.) were established under eight different hormonal supplementations and proliferated by subculture of nodal shoot segments. The respective essential oils, obtained by hydrodistillation, were composed of more than 75 compounds, 65 of which were identified. The 10 major compounds were, by order of retention time, alpha-pinene (4.1-5.4%), camphene (6-7.1%), beta-pinene (9.3-14.5%), limonene (2-2.3%), 1,8-cineole (3.6-5.6%), (-)-thujone (13.2-16.1%), (+)-isothujone (6.6-7.4%), camphor (19.8-24%), alpha-humulene (5.1-6.8%), and manool (4.2-7.7%). Notwithstanding the eight different hormonal supplementations tested, the percentage composition of the shoot essential oils were kept in a narrow range of variation. However, the type and concentration of growth regulators apparently influenced the accumulation of essential oils. The highest accumulation of essential oils and the highest shoot biomass growth were obtained with 2.0 mg/L kinetin and 0.05 mg/L 2,4-dichlorophenoxyacetic acid.
Santos-Gomes
Paula C
PC
Universidade do Minho, Departamento de Biologia, Escola de Ciências, Largo do Paço, 4709 Braga Codex, Portugal.
Fernandes-Ferreira
Manuel
M
D000081008:Bicyclic Monoterpenes; D001643:Bridged Bicyclo Compounds / Q000032:analysis; D002164:Camphor / Q000032:analysis; D002849:Chromatography, Gas / Q000379:methods; D053138:Cyclohexenes; D008401:Gas Chromatography-Mass Spectrometry; D000077222:Limonene; D039821:Monoterpenes / Q000032:analysis; D009822:Oils, Volatile / Q000737:chemistry*; D027543:Salvia officinalis / Q000737:chemistry* / Q000254:growth & development; D013729:Terpenes / Q000032:analysis
D000081008:Bicyclic Monoterpenes; D001643:Bridged Bicyclo Compounds; D053138:Cyclohexenes; D039821:Monoterpenes; D009822:Oils, Volatile; D013729:Terpenes; C010789:beta-pinene; D002164:Camphor; C005790:beta-thujone; D000077222:Limonene; C019286:camphene; C005451:alpha-pinene