title: Cytogenetic and molecular characterization of the MBSAT1 satellite DNA in holokinetic chromosomes of the cabbage moth, Mamestra brassicae (Lepidoptera).
abstract: Digestion of Mamestra brassicae DNA with DraI produced a prominent fragment of approximately 200 bp and a ladder of electrophoretic bands with molecular weights which are a multiple of 200 bp. Southern blotting revealed that this ladder is composed of DNA fragments that are multimers of the 200-bp DraI band suggesting that DraI isolated a satellite that has been called Mamestra brassicae satellite DNA1 (MBSAT1). MBSAT1 is the first satellite DNA isolated in Lepidoptera. In-situ DraI digestion of chromosome spreads, together with fluorescent in-situ hybridization, showed that MBSAT1 sequences are clustered in heterochromatin of the sex chromosomes, Z and W. MBSAT1 was 234 bp long with an AT content of 60.7%. The curvature-propensity plot suggested a curvature in the MBSAT1 structure.
authors.affiliation: Dipartimento di Biologia Animale, Università di Modena e Reggio Emilia, Via Campi 213/D, 41100 Modena, Italy. mandriol@unimo.it
authors.forename: Mauro
authors.initials: M
authors.lastname: Mandrioli
authors.forename: Gian Carlo
authors.initials: GC
authors.lastname: Manicardi
authors.forename: Frantisek
authors.initials: F
authors.lastname: Marec
mesh_terms: D000818:Animals; D002875:Chromosomes; D004276:DNA, Satellite; D017404:In Situ Hybridization, Fluorescence; D008677:Metaphase; D008938:Mitosis; D009036:Moths / Q000235:genetics*
chemical_list: D004276:DNA, Satellite