title: [Efficacy and impact of polymerase chain reaction in the diagnosis of extrapulmonary tuberculosis].
abstract: OBJECTIVE
  To evaluate the efficacy of PCR in the diagnosis of extrapulmonary tuberculosis (TB) and its impact in the management of patients in a tertiary-care center in Mexico City.
  
  
  METHODS
  We conducted a retrospective study based on 40 clinical charts of patients to whom nested PCR was performed for the diagnosis of TB from June 1999 to December 2000. We reviewed the medical notes of 10 days before and 10 days after the PCR study to analyze its impact in the management of the patient. Also, we reviewed the rest of the chart to decide if the patient suffered from TB or not (gold standard). The categories of diagnosis were definitive case of TB, probable TB and no TB. We calculated the sensitivity, specificity, and predictive values.
  
  
  RESULTS
  The PCR was positive in 45% of the cases. The sensitivity of PCR to diagnosis TB was 50%, specificity 59%, and the positive and negative predictive value were 35% and 72%, respectively. If just spinal fluid was included, the sensitivity and the negative predictive value increased to 75% and 63%, respectively. The PCR had an impact in the management of 13% of the patients.
  
  
  CONCLUSIONS
  The PCR for the diagnosis of extrapulmonary TB has limited efficacy, which improves when the test is done in spinal fluid samples. The impact of the result of PCR in the clinical management of the patients was poor.
authors.affiliation: Laboratorio de Microbiología Clínica, Departamento de Infectología, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, México, D.F.
authors.forename: Midori
authors.initials: M
authors.lastname: Kato Maeda
authors.forename: Miriam
authors.initials: M
authors.lastname: Bobadilla Del Valle
authors.forename: Areli
authors.initials: A
authors.lastname: Martínez Gamboa
authors.forename: Araceli
authors.initials: A
authors.lastname: Hernández Cruz
authors.forename: Isabel
authors.initials: I
authors.lastname: Ramírez Mora
authors.forename: Norma
authors.initials: N
authors.lastname: Cerón Enríquez
authors.forename: Narciso
authors.initials: N
authors.lastname: Ortiz Conchi
authors.forename: Luis Rodrigo
authors.initials: LR
authors.lastname: Macías Kauffer
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Toral Villanueva
authors.forename: Alfredo
authors.initials: A
authors.lastname: Ponce de León
authors.forename: José
authors.initials: J
authors.lastname: Sifuentes Osornio
mesh_terms: D006801:Humans; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000175:diagnosis*