In vitro properties of RpoS (sigma(S)) mutants of Escherichia coli with postulated N-terminal subregion 1.1 or C-terminal region 4 deleted.
Derivatives of the stationary-phase sigma factor sigma(S) of Escherichia coli lacking either of two conserved domains, the postulated N-terminal subregion 1.1 or the C-terminal region 4, were shown to be competent in vitro for transcription initiation from several sigma(S)-dependent promoters on supercoiled DNA templates. Unlike wild-type sigma(S), however, the deletion derivatives were inactive on relaxed templates. The anomalous slow electrophoretic mobility of sigma(S) on denaturing gels was corrected by deletion of subregion 1.1, suggesting that this domain in sigma(S) may be structurally and functionally analogous to subregion 1.1 of sigma(70), substitutions in which have previously been shown to rectify the anomalous electrophoretic migration of sigma(70) (V. Gopal and D. Chatterji, Eur. J. Biochem. 244:614-618, 1997).
Gowrishankar
J
J
Laboratory of Bacterial Genetics, Centre for DNA Fingerprinting and Diagnostics, Hyderabad 500 076, India. shankar@cdfd.org.in
Yamamoto
Kaneyoshi
K
Subbarayan
P R
PR
Ishihama
Akira
A
D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics*; D017353:Gene Deletion; D011401:Promoter Regions, Genetic; D021122:Protein Subunits / Q000737:chemistry; D012808:Sigma Factor / Q000737:chemistry / Q000235:genetics*; D013698:Templates, Genetic; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D021122:Protein Subunits; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria