Comparative development of Eimeria tenella (Apicomplexa) in host cells in vitro.
Attempts to propagate Eimeria tenella in cell culture over the years have met with limited success. The host cell type is an important parameter in such cultures. This study assessed the ability of different host cell lines to support E. tenella infection in vitro. The initial development in cell lines and the effects of incubation at 37 degrees C and 41 degrees C on the host-parasite relationship was investigated. Eleven cell lines were seeded into 96 well plates and incubated at 37 degrees C in 5% CO(2) to reach confluency. Sporozoites of E. tenella were inoculated into wells and allowed to invade at 37 degrees C and 41 degrees C. Intracellular parasite development was quantified using (3)H-uracil incorporation. All cell lines facilitated parasite invasion and development. The MDBK cell line supported the highest degree of E. tenella development. A time-course study was undertaken to look at this host cell-parasite relationship during infection.
Tierney
J
J
Department of Veterinary Microbiology and Parasitology, Faculty of Veterinary Medicine and Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland. Joanna.Tierney@ucd.ie
Mulcahy
G
G
D000818:Animals; D018938:Caco-2 Cells; D002415:Cats; D002417:Cattle; D002460:Cell Line; D002522:Chlorocebus aethiops; D006224:Cricetinae; D004285:Dogs; D016786:Eimeria tenella / Q000254:growth & development* / Q000472:pathogenicity*; D006790:Host-Parasite Interactions; D006801:Humans; D010274:Parasitology / Q000379:methods; D011817:Rabbits; D051381:Rats; D013696:Temperature; D014709:Vero Cells