title: Measurement of the kinetic isotope effect for the oxidation of NADH at a poly(aniline)-modified electrode.
abstract: Kinetic isotope measurements using [4,4-2H2]NADH and [4-1H, 4-2H]NADH have been used to investigate the mechanism of the electrochemical oxidation of NADH at poly(aniline)-poly(vinyl sulfonate)-modified electrodes. The experiments show a primary kinetic isotope effect for the reaction of 4.2. This is consistent with literature values for the corresponding isotope effect for the oxidation of NADH by two-electron oxidants in homogeneous solution. The result demonstrates that transfer of H from NADH to the modified electrode occurs in the rate-limiting step within the reaction complex.
authors.affiliation: Department of Chemistry, University of Southampton, Southampton, SO17 1BJ, UK. p.n.bartlett@soton.ac.uk
authors.forename: Philip N
authors.initials: PN
authors.lastname: Bartlett
authors.forename: Evelyne
authors.initials: E
authors.lastname: Simon
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry*; D003903:Deuterium; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D007700:Kinetics; D009243:NAD / Q000737:chemistry* / Q000378:metabolism; D010084:Oxidation-Reduction
chemical_list: D000814:Aniline Compounds; C416807:polyaniline; D009243:NAD; D003903:Deuterium