Effect of chlorpromazine free radical on tyrosine hydroxylase of bovine adrenal medulla.
The effect of preincubation with chlorpromazine and chlorpromazine free radical on tyrosine hydroxylase activity was studied. Chlorpromazine free radical inhibited the enzyme activity to a greater extent than chlorpromazine. More than a two-fold decrease in the enzyme activity was observed following the preincubation of the enzyme with chlorpromazine free radical. This observation may suggest interaction between chlorpromazine free radical and -SH group located on the active site of the apoenzyme. Another interaction between chlorpromazine free radical and the coenzyme (DMPH4) was confirmed from spectral studies.
Mostafa
M H
MH
Medical Research Institute, Alexandria University, Alexandria.
el-Sewedy
S M
SM
Rakonczay
Z
Z
D000313:Adrenal Medulla / Q000187:drug effects / Q000201:enzymology*; D000818:Animals; D014150:Antipsychotic Agents / Q000737:chemistry / Q000494:pharmacology*; D002417:Cattle; D002746:Chlorpromazine / Q000737:chemistry / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005609:Free Radicals; D066298:In Vitro Techniques; D014446:Tyrosine 3-Monooxygenase / Q000037:antagonists & inhibitors*
D014150:Antipsychotic Agents; D004791:Enzyme Inhibitors; D005609:Free Radicals; D014446:Tyrosine 3-Monooxygenase; D002746:Chlorpromazine