title: cDNA encoding protein O-mannosyltransferase from the filamentous fungus Trichoderma reesei; functional equivalence to Saccharomyces cerevisiae PMT2.
abstract: O-Mannosylation is suggested to be essential for protein secretion in Trichoderma reesei. In protein O-glycosylation, the first mannosyl residue is transferred to a serine or threonine hydroxyl group of the protein from dolichyl phosphate mannose by protein O-mannosyltransferase. In Saccharomyces cerevisiae, seven PMT genes have been cloned coding for these enzymes. In the present work, the characterisation of the pmt1 cDNA from T. reesei is reported. Sequence analysis of the predicted protein revealed the highest similarity to Schizosaccharomyces pombe Pmt and to Pmt4p of Saccharomyces cerevisiae. In contrast, expression of the T. reesei cDNA in various S. cerevisiae pmt mutants showed functional similarity to the yeast Pmt2 protein.
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Zakrzewska
authors.forename: Andrzej
authors.initials: A
authors.lastname: Migdalski
authors.forename: Markku
authors.initials: M
authors.lastname: Saloheimo
authors.forename: Merja E
authors.initials: ME
authors.lastname: Penttila
authors.forename: Grazyna
authors.initials: G
authors.lastname: Palamarczyk
authors.forename: Joanna S
authors.initials: JS
authors.lastname: Kruszewska
mesh_terms: D000595:Amino Acid Sequence; D018076:DNA, Complementary; D008364:Mannosyltransferases / Q000235:genetics*; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D014242:Trichoderma / Q000201:enzymology / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D008364:Mannosyltransferases; C082696:protein O-mannosyltransferase