Dna2 requirement for normal reproduction of Caenorhabditis elegans is temperature-dependent.
To determine the function of Dna2 in a multicellular organism, the Caenorhabditis elegans Dna2 expression was probed and deletion mutant phenotypes were analyzed. Dna2 was localized to the nuclei of C. elegans oocytes and early embryos by immunostaining or green fluorescent protein-tagging. A homozygous dna2 deletion mutant showed a reduced brood size and embryonic lethality, and the phenotypes greatly depended on growth temperature and aggravated in the succeeding generation. The mutant embryos also showed delayed cell divisions, which together with temperature-dependence of the mutant phenotypes supported the well-conserved role of Dna2 in DNA replication.
Lee
Kyoung-Hwa
KH
Department of Biochemistry, College of Science, Yonsei University, Seoul 120-749, Korea.
Lee
Myon Hee
MH
Lee
Tae Hoon
TH
Han
Ji Won
JW
Park
Yong Jin
YJ
Ahnn
Joohong
J
Seo
Yeon-Soo
YS
Koo
Hyeon-Sook
HS
D000251:Adenosine Triphosphatases / Q000235:genetics / Q000502:physiology*; D000595:Amino Acid Sequence; D000818:Animals; D020132:Anticipation, Genetic; D017173:Caenorhabditis elegans / Q000196:embryology / Q000235:genetics / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000502:physiology*; D002455:Cell Division; D004265:DNA Helicases / Q000235:genetics / Q000502:physiology*; D004261:DNA Replication; D018509:DNA, Helminth / Q000096:biosynthesis / Q000235:genetics; D005298:Fertility / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D005804:Genes, Lethal; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000032:analysis / Q000235:genetics; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism; D034622:RNA Interference; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013696:Temperature
D029742:Caenorhabditis elegans Proteins; D018509:DNA, Helminth; D008164:Luminescent Proteins; D029701:Saccharomyces cerevisiae Proteins; D049452:Green Fluorescent Proteins; D000251:Adenosine Triphosphatases; D004265:DNA Helicases; C410222:DNA2 protein, S cerevisiae