Immunocytochemical localization of prostaglandin F synthase II in the rat spinal cord.
Prostaglandin F synthase has at least two isozymes, i.e. prostaglandin F synthase I and II. Recently, we demonstrated immunocytochemically that prostaglandin F synthase I was localized in neuronal dendrites and somata, and in endothelial cells of blood vessels in the whole area of rat spinal cord. In the present study, we immunocytochemically localized prostaglandin F synthase II in ependymal cells and tanycytes surrounding the central canal and in endothelial cells of blood vessels, but not in any neuronal elements at all segmental levels of the rat spinal cord. Immunoelectron microscopy and confocal laser scanning microscopy confirmed these findings and further revealed that strong immunoreactivity was found in the basal processes of the tanycytes. Our present and recent studies using antibodies against the two isozymes of prostaglandin F synthase clearly indicated that they were localized differentially in ependymal (prostaglandin F synthase II) and neuronal elements (prostaglandin F synthase I), but were co-localized in blood vessels in the rat spinal cord. The distinct localization of the two isozymes suggests that prostaglandin F(2) has different transcellular biological actions via different cell groups.
Suzuki-Yamamoto
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Department of Anatomy and Cell Biology, The University of Tokushima, School of Medicine, Kuramoto, 770-8503, Tokushima, Japan. toshiko@basic.med.tokushima-u.ac.jp
Toida
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K
Watanabe
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Ishimura
K
K
D000818:Animals; D004730:Endothelium, Vascular / Q000201:enzymology / Q000648:ultrastructure; D004805:Ependyma / Q000201:enzymology / Q000648:ultrastructure; D006911:Hydroxyprostaglandin Dehydrogenases / Q000378:metabolism*; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D018613:Microscopy, Confocal; D016253:Microscopy, Immunoelectron; D009474:Neurons / Q000201:enzymology / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D013116:Spinal Cord / Q000166:cytology* / Q000201:enzymology* / Q000648:ultrastructure
D007527:Isoenzymes; D006911:Hydroxyprostaglandin Dehydrogenases; C031051:prostaglandin-F synthase