Reductive dechlorination of weathered Aroclor 1260 during anaerobic biotreatment of Arctic soils.
We investigated the microbial reductive dechlorination of both weathered (aged) and nonweathered (freshly added) Aroclor 1260 in aerobic soil from Resolution Island, Nunavut, Canada. Initial polychlorinated biphenyl (PCB) concentrations were 106 and 100 ppm, respectively. The aerobic soil samples were inoculated with anaerobic sediment, incubated at 30 degrees C until methanogenic, inoculated with a dechlorinating enrichment culture, and incubated a further 8 weeks. The average number of chlorine substituents per biphenyl molecule was biologically reduced from 6.6 to 5.1 and from 6.2 to 4.5 for weathered and nonweathered Aroclor 1260, respectively. Removal of hexa- and heptachlorobiphenyls (CBs), the major homolog groups present, was significantly greater for nonweathered than for weathered Aroclor 1260. Formation of dechlorination products, primarily 2,2',4,4'- and 2,2',4,6'-tetraCBs, was also significantly greater for nonweathered than for weathered Aroclor 1260. We additionally compared the dechlorination at 21 degrees C of weathered Aroclor 1260 in soils from Resolution Island and Saglek, Labrador, Canada. The average number of chlorine substituents per biphenyl molecule was biologically reduced from 6.7 to 5.1 and from 6.5 to 4.6, respectively. This study demonstrated the potential for bioremediation of aerobic soil contaminated with Aroclor 1260 and showed that weathering may limit such treatment to an extent variable among different soils.
Kuipers
Bianca
B
Environmental Chemistry Group, Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, BC, V6T 1Z1, Canada.
Cullen
William R
WR
Mohn
William W
WW
D001110:Arctic Regions; D001140:Aroclors / Q000378:metabolism*; D001420:Bacteria, Aerobic / Q000378:metabolism / Q000502:physiology*; D001673:Biodegradation, Environmental; D002713:Chlorine / Q000737:chemistry*; D004785:Environmental Pollutants / Q000378:metabolism*; D011078:Polychlorinated Biphenyls / Q000494:pharmacology; D012989:Soil Pollutants / Q000378:metabolism*
D001140:Aroclors; D004785:Environmental Pollutants; D012989:Soil Pollutants; C026987:aroclor 1260; D002713:Chlorine; D011078:Polychlorinated Biphenyls