PCR detection of Porcine circovirus type 2 DNA in whole blood, serum, oropharyngeal swab, nasal swab, and feces from experimentally infected pigs and field cases.
Porcine circovirus type 2 (PCV2) shedding patterns were investigated by polymerase chain reaction (PCR) for the detection of PCV2 DNA, and the diagnostic suitability of a sample for the PCR was examined by using different types of samples. In the experimental infection, sixteen pigs were inoculated intranasally with PCV2. The samples, including oropharyngeal and nasal swabs, feces, whole blood and serum became positive for PCV2 DNA by PCR immediately after the inoculation, and almost all samples remained positive during the observation period, post-inoculation-day 70. Field samples were collected from 313 pigs in five different age groups. The overall percentages of positive samples in the whole blood, nasal swabs, and feces detected by PCR were 30.4%, 19.2%, and 20.4%, respectively. The frequency of positive samples increased after the nursery stages and reached a peak in the 3 to 4-month-old pigs. These results indicate that PCV2 infection may occur after weaning, that PCV2 DNA may be present in whole blood for a long period after infection, and that whole blood and serum are the most suitable sample types for the PCR analysis of PCV2.
Shibata
Isao
I
Zen-noh Institute of Animal Health, Chiba, Japan.
Okuda
Yo
Y
Yazawa
Shigeto
S
Ono
Masaaki
M
Sasaki
Takashi
T
Itagaki
Mitsuaki
M
Nakajima
Nobuaki
N
Okabe
Yukinobu
Y
Hidejima
Isao
I
D000375:Aging; D000818:Animals; D018173:Circoviridae Infections / Q000097:blood / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000032:analysis* / Q000097:blood* / Q000235:genetics; D005243:Feces / Q000821:virology*; D009296:Nasal Cavity / Q000821:virology*; D009960:Oropharynx / Q000821:virology*; D016133:Polymerase Chain Reaction; D013552:Swine; D013553:Swine Diseases / Q000821:virology*
D004279:DNA, Viral