[Determination of echinacoside in congrong spirit by reversed-phase high performance liquid chromatography].
A reversed-phase high performance liquid chromatographic method, which is relatively simple, rapid and effective for echinacoside determination in Congrong spirit, has been developed. With this method, the peak of echinacoside could be separated completely from other interfering components in the spirit. The chromatographic conditions used were a reversed-phase C18 column at 25 degrees C with detection wavelength at 330 nm and CH3CN-1% HAc (14:86 in volume ratio) as mobile phase. A good linear relationship has been obtained between 0.52 microgram to 2.60 micrograms of echinacoside. The recovery was 99.7%.
Lei
Li
L
School of Pharmaceutical Sciences, Modern Research Center of Chinese Traditional Medicines, Peking University, Beijing 100083, China.
Tu
Peng-fei
PF
Zhang
Jian-guo
JG
Zhai
Yan-kui
YK
Yu
Shi-chen
SC
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D031671:Cistanche / Q000737:chemistry*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D006027:Glycosides / Q000032:analysis*; D014920:Wine / Q000032:analysis*
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D006027:Glycosides; C060297:echinacoside