The structure of lipid A of the lipopolysaccharide from Burkholderia caryophylli with a 4-amino-4-deoxy-L-arabinopyranose 1-phosphate residue exclusively in glycosidic linkage.
From the lipopolysaccharides (LPSs) of the plant-pathogenic bacterium Burkholderia caryophylli, the complete structure of lipid A has been characterized. For the first time, a 4-amino-4-deoxy-L-arabinopyranose 1-phosphate residue was proven to be exclusively linked to the reducing end of lipid A from a wild-type LPS. The LPSs of B. caryophylli were degraded by mild acetate buffer hydrolysis at pH 4.4. The obtained lipid A was analyzed as such, and also after de-O-acylation or dephosphorylation. The structure of lipid A was identified mainly by means of matrix-assisted laser desorption/ionisation mass spectrometry, and by various 1D and 2D (1)H and (13)C NMR spectroscopic measurements.
Molinaro
Antonio
A
Dipartimento di Chimica Organica e Biochimica, Università degli studi di Napoli Federico II, Italy.
Lindner
Buko
B
De Castro
Cristina
C
Nolting
Birte
B
Silipo
Alba
A
Lanzetta
Rosa
R
Parrilli
Michelangelo
M
Holst
Otto
O
D000215:Acylation; D001089:Arabinose / Q000737:chemistry*; D019117:Burkholderia / Q000737:chemistry* / Q000378:metabolism; D002236:Carbohydrate Conformation; D006027:Glycosides / Q000737:chemistry*; D008050:Lipid A / Q000737:chemistry* / Q000378:metabolism; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010755:Organophosphates / Q000737:chemistry*; D010766:Phosphorylation; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D006027:Glycosides; D008050:Lipid A; D010755:Organophosphates; D001089:Arabinose