Molecular cloning and expression analysis of the mouse Spot-2 gene in pituitary development.
Mouse Spot-1 is a DNA-binding protein with a domain (His-Thr) encoded by p(CA)n repeats. Spot-1 interacts with the nuclear localization signal (NLS) I of p53 through its His-Thr domain. In this study we describe the cloning and expression patterns of a novel gene encoding a protein containing a His-Thr domain, Spot-2. Spot-2 is exclusively expressed in the pituitary from stage E13.5 to E15.5. Mouse Lhx3 plays a critical role during early organogenesis in the pituitary. The Spot-2 gene appears to be a downstream gene of Lhx3. It is suggested that Spot-2 plays important roles in pituitary development.
Miyamoto
Toshinobu
T
Department of Obstetrics and Gynecology, Asahikawa Medical College, Asahikawa, 078-8510, Hokkaido, Japan. toshim@asahikawa-med.ac.jp
Hasuike
Shiga
S
Sengoku
Kazuo
K
Takuma
Naoyuki
N
Hayashi
Hiroaki
H
Sasaki
Yoshihito
Y
Yamashita
Tsuyoshi
T
Ishikawa
Mutsuo
M
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D060850:LIM-Homeodomain Proteins; D051379:Mice; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D019913:Nuclear Localization Signals; D010902:Pituitary Gland / Q000196:embryology* / Q000502:physiology; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D060850:LIM-Homeodomain Proteins; C094898:Lhx3 protein; D019913:Nuclear Localization Signals; C481358:Spot-2 protein, mouse; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53