An efficient fusion expression system for protein and peptide overexpression in Escherichia coli and NMR sample preparation.
An efficient fusion expression system with a small fusion partner, His6-tagged N-terminal fragment of staphylococcal nuclease R, has been constructed and tested with two genes. The results show that the system is not only suitable for overexpression of small proteins and peptides but simplifies purification of target proteins and peptides. The study also provides a practical method for preparation of isotope-labeled protein sample for NMR analysis.
Cheng
Yuan
Y
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
Liu
Dongsheng
D
Feng
Yanming
Y
Jing
Guozhong
G
D003435:Crotalid Venoms / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics*; D009682:Magnetic Resonance Spectroscopy; D009363:Neoplasm Proteins / Q000235:genetics / Q000302:isolation & purification; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification
D003435:Crotalid Venoms; D009363:Neoplasm Proteins; D011993:Recombinant Fusion Proteins; C018932:myotoxin A