Whole CagA gene amplification of Helicobacter pylori and its fingerprinting by restriction fragment length polymorphism.
To set up a method of amplification for the whole CagA gene of Helicobacter pylori and its fingerprinting by restriction fragment length polymorphism (RFLP), nested PCR was employed in combination with TD-PCR to amplify the gene and EcoRI and Hind III were used to generate the RFLP fingerprinting. Target DNA fragments from 13 of 20 samples were successfully amplified and the relevant RFLP fingerprintings were obtained. It is concluded that the method can be used to amplify the whole CagA gene and CagA gene has apparent diversity of RFLP profile.
Ye
Siying
S
Department of Pathogenic Organisms, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
Ao
Jienan
J
Peng
Ying
Y
Yue
Haifeng
H
Liao
Fang
F
Hu
Guoping
G
Xu
Yang
Y
Zhang
Zhengmao
Z
D000942:Antigens, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D016172:DNA Fingerprinting / Q000379:methods; D005784:Gene Amplification / Q000235:genetics*; D016480:Helicobacter pylori / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C080866:cagA protein, Helicobacter pylori