Expression of CD83 in the murine immune system.
CD83 is used as a marker for mature dendritic cells (DC) in man. We have developed a new monoclonal antibody (mAb), Michel-17, that specifically recognizes mouse CD83. We show that murine CD83 is expressed mainly on mature DC and on activated T cells. Histological analysis of serial spleen sections revealed a CD83 expression pattern resembling that of MIDC-8, a known murine DC marker molecule. In contrast to other costimulatory receptors, cross-linking of CD83 with the mAb Michel-17 on DC or T cells does not induce any activation signals. Our data describe for the first time the expression pattern of murine CD83, which is comparable to that of human CD83. The unique mAb Michel-17 will help to elucidate the biological functions of the CD83 molecule in more detail.
Wolenski
Matthias
M
Bernhard-Nocht-Institute for Tropical Medicine, Bernhard-Nocht-Strasse 74, 20359 Hamburg, Germany.
Cramer
Sven O
SO
Ehrlich
Svenja
S
Steeg
Christiane
C
Grossschupff
Gudrun
G
Tenner-Racz
Klara
K
Racz
Paul
P
Fleischer
Bernhard
B
von Bonin
Arne
A
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD; D018929:Cell Culture Techniques; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D007136:Immunoglobulins / Q000096:biosynthesis* / Q000235:genetics*; D018664:Interleukin-12 / Q000032:analysis; D007376:Interleukin-2 / Q000032:analysis; D008070:Lipopolysaccharides / Q000276:immunology; D008099:Liver / Q000166:cytology; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D000097802:CD83 Antigen
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D007136:Immunoglobulins; D018664:Interleukin-12; D007376:Interleukin-2; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D000097802:CD83 Antigen