Modulation of BmK AS, a scorpion neurotoxic polypeptide, on voltage-gated Na+ channels in B104 neuronal cell line.
The modulation of BmK AS, a neurotoxic polypeptide from scorpion Buthus martensi Karsch, on the voltage-gated Na(+) channels has been investigated in the B104 neuroblastoma cell line by whole-cell patch clamping. It was found that the whole Na(+) currents and tetrodotoxin-resistant Na(+) current were depressed in a biphasic manner and both steady-state activation and inactivation curves were shifted toward the hyperpolarizing direction in the presence of BmK AS. The results suggested that BmK AS could inhibit both tetrodotoxin-sensitive and -resistant Na(+) currents via modulating the gating mechanism of the voltage-gated Na(+) channels in B104 cells.
Tan
Zhi-Yong
ZY
The Key Laboratory of Neuroscience, Institute of Physiology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, China.
Chen
Jin
J
Shun
Hai-Ying
HY
Feng
Xing-Hua
XH
Ji
Yong-Hua
YH
D000818:Animals; D004305:Dose-Response Relationship, Drug; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D010455:Peptides / Q000633:toxicity*; D051381:Rats; D012604:Scorpion Venoms / Q000633:toxicity*; D015222:Sodium Channels / Q000502:physiology*; D013779:Tetrodotoxin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
C119407:BmK AS polypeptide; D010455:Peptides; D012604:Scorpion Venoms; D015222:Sodium Channels; D013779:Tetrodotoxin