Protective effect of three diallyl sulphides against glucose-induced erythrocyte and platelet oxidation, and ADP-induced platelet aggregation.
Isolated human erythrocyte membrane and platelet were used to study the antioxidative and anti-aggregative effects of three diallyl sulphides (diallyl sulphide, DAS; diallyl disulphide, DADS; diallyl trisulphide, DAT) against glucose-induced oxidation and adenosine 5'-diphosphate (ADP)-induced platelet aggregation. Three sulphide agents showed dose-dependent antioxidative protection against glucose-induced erythrocyte membrane oxidation (p<.05), and these agents at 10 microM significantly increased the retention of alpha-tocopherol in erythrocyte membrane (p<.05), in which DAT was the most effective agent (p<.05). Three sulphide agents significantly inhibited 30 and 50 mM of glucose-induced platelet oxidation (p<.05). The anti-aggregative activity of each sulphide agent was dose dependent (p<.05), in which DAT showed the greatest inhibitory effect on platelet aggregation than DADS, followed by DAS (p<.05). After ADP stimulation, the malondialdehyde (MDA) formation in platelets treated with sulphide agents was significantly less (p<.05), in which DADS and DAT showed similar antioxidative activities (p>.05). These results suggested that DADS and DAT could be considered as strong antioxidative and antithrombotic agents to prevent or control oxidative damage and platelet hyperactivity.
Chan
Kung-chi
KC
Department of Food and Nutrition, Providence University, Taichung, Taiwan, ROC.
Hsu
Cheng-chin
CC
Yin
Mei-chin
MC
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000328:Adult; D000498:Allyl Compounds / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D004220:Disulfides / Q000494:pharmacology; D004336:Drug Antagonism; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D008315:Malondialdehyde / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects; D013440:Sulfides / Q000494:pharmacology*; D024502:alpha-Tocopherol / Q000378:metabolism
D000498:Allyl Compounds; D000975:Antioxidants; D004220:Disulfides; D013440:Sulfides; C042577:diallyl trisulfide; D008315:Malondialdehyde; C028009:diallyl disulfide; C038491:allyl sulfide; D000244:Adenosine Diphosphate; D024502:alpha-Tocopherol; D005947:Glucose