Estimation of potential and limits of bivalve closure response to detect contaminants: application to cadmium.
Bivalve closure responses to detect contaminants have often been studied in ecotoxicology as an aquatic pollution biosensor. We present a new laboratory procedure to estimate its potential and limits for various contaminants and animal susceptible to stress. The study was performed in the Asiatic clam Corbicula fluminea and applied to cadmium. To take into account the rate of spontaneous closures, we integrated stress problems associated with fixation by a valve in common apparatus and the spontaneous rhythm associated with circadian activity to focus on conditions with the lowest probability of spontaneous closing. Moreover, we developed an original system by impedance valvometry, using light-weight impedance electrodes, to study free-ranging animals in low-stress conditions and a new analytical approach to describe valve closure behavior as a function of response time and concentration of contaminant. In C. fluminea, we show that cadmium concentrations above 50 microg/L can be detected within less than 1 h, concentrations down to 16 microg/L require 5 h of integration time, and values lower than 16 microg/L cannot be distinguished from background noise. Our procedure improved by a factor of six the cadmium sensitivity threshold reported in the literature. Problems of field applications are discussed.
Tran
Damien
D
Unité Mixte de Recherche 5805, Laboratoire d'Ecophysiologie et Ecotoxicologie des Systèmes Aquatiques, University Bordeaux 1, CNRS, Place du Dr Peyneau 33120, Arcachon, France.
Ciret
Pierre
P
Ciutat
Aurélie
A
Durrieu
Gilles
G
Massabuau
Jean-Charles
JC
D000818:Animals; D049872:Bivalvia / Q000187:drug effects* / Q000502:physiology*; D002104:Cadmium / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D009043:Motor Activity / Q000187:drug effects*; D010507:Periodicity; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D014874:Water Pollutants, Chemical; D002104:Cadmium