Synthesis of chemotactic peptide analogs with a photoaffinity cross-linker as new probes for analysis of receptor-ligand interaction.
Formylpeptide receptors are well-characterized receptors which participate in host defense responses of neutrophils. We designed and synthesized chemotactic peptide analog with p-benzoylphenylalanine (Bpa) and biotin to probe structural and mechanistic aspects of peptide-receptor interaction. These peptides possess biological activities which were dependent upon spacer residue length of and Bpa position. The covalent photoaffinity label was detected by Streptavidine-blot, which was inhibited by the parent peptide.
Shibue
Mitsukuni
M
Micro-space Chemistry Laboratory, AIST Kyushu, National Institute of Advanced Industrial Science and Technology, Tosu, Saga, Japan.
Yoshiki
Masahiro
M
Miyazaki
Masaya
M
Fujita
Ichiro
I
Osada
Satoshi
S
Yasuda
Seiji
S
Nakamura
Hiroyuki
H
Hamasaki
Yuhei
Y
Kondo
Michio
M
Maeda
Hideaki
H
Kodama
Hiroaki
H
Shimizu
Hazime
H
D001710:Biotin / Q000737:chemistry; D002633:Chemotaxis / Q000502:physiology*; D015335:Molecular Probes; D010455:Peptides / Q000138:chemical synthesis* / Q000378:metabolism; D010649:Phenylalanine / Q000031:analogs & derivatives* / Q000737:chemistry; D019920:Photoaffinity Labels; D015202:Protein Engineering
D015335:Molecular Probes; D010455:Peptides; D019920:Photoaffinity Labels; D010649:Phenylalanine; D001710:Biotin; C053764:benzoylphenylalanine