title: Genetic alterations detected on chromosomes 13 and 14 in Chinese non-small cell lung carcinomas.
abstract: Genetic alterations in 28 non-small cell lung carcinoma patients were detected on chromosomes 13q and 14q with microsatellite markers by polymerase chain reaction techniques. Loss of heterozygosity of up to 50% was detected with chromosome 13 markers and of up to 37% for chromosome 14. Microsatellite instability was as high as 30% on chromosome 13 and up to 19% on chromosome 14. Accumulated mutation frequencies of up to 94 and 93% were observed for chromosomes 13 and 14, respectively. Of eight tumors displaying high mutation frequencies, 1 also carried a K-ras mutation and 4 had p53 mutations. A significant association was observed between p53 mutations and genetic instability.
authors.affiliation: Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong (SAR), PR China.
authors.forename: Fung Mei
authors.initials: FM
authors.lastname: Kwong
authors.forename: Po Shan
authors.initials: PS
authors.lastname: Wong
authors.forename: Maria Li
authors.initials: ML
authors.lastname: Lung
mesh_terms: D000328:Adult; D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D002681:China; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics*; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics*; D004252:DNA Mutational Analysis; D005260:Female; D016158:Genes, p53 / Q000235:genetics; D011905:Genes, ras / Q000235:genetics; D020022:Genetic Predisposition to Disease; D006801:Humans; D019656:Loss of Heterozygosity / Q000235:genetics; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D008875:Middle Aged; D009154:Mutation / Q000235:genetics; D009367:Neoplasm Staging