Use of single-enzyme amplified fragment length polymorphism for typing Pasteurella multocida subsp. multocida isolates from pigs.
Single-enzyme amplified fragment length polymorphism (SE-AFLP) analyses were used to differentiate 97 isolates of porcine Pasteurella multocida subsp. multocida. The strains, isolated from animals with pneumonia, rhinitis, and septicemia, were classified as capsular types A, D, and F. SE-AFLP showed a discriminatory index of 0.87 and identified 18 different profiles.
Moreno
A M
AM
Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Sao Paulo, Brazil. morenoam@uol.com.br
Baccaro
M R
MR
Ferreira
A J P
AJ
Pestana De Castro
A F
AF
D000818:Animals; D016470:Bacteremia / Q000382:microbiology / Q000662:veterinary; D016667:Bacterial Capsules / Q000276:immunology; D001427:Bacterial Toxins / Q000235:genetics; D015373:Bacterial Typing Techniques; D015247:Deoxyribonuclease HindIII / Q000378:metabolism*; D010326:Pasteurella Infections / Q000382:microbiology / Q000662:veterinary*; D016979:Pasteurella multocida / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D018410:Pneumonia, Bacterial / Q000382:microbiology / Q000662:veterinary; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012222:Rhinitis, Atrophic / Q000382:microbiology / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
D001427:Bacterial Toxins; D015247:Deoxyribonuclease HindIII