Optimization of a solid-phase extraction method for determination of indapamide in biological fluids using high-performance liquid chromatography.
A new simple and rapid high-performance liquid chromatographic (HPLC) method with UV detection for the determination of indapamide in biological fluids has been developed. Indapamide and internal standard were isolated from serum and whole blood samples by solid-phase extraction with RP select B cartridges. The chromatographic separation was accomplished on a reversed-phase C(8) column with a mobile phase composed of 0.1% (v/v) triethylamine in water (pH 3.5) and acetonitrile (63:37, v/v). UV detection was set at 240 nm. The calibration curves were linear in the concentration range of 10.0-100.0 ng/ml for serum, and 50.0-500.0 ng/ml for whole blood, and the limits of quantification were 10.0 and 50.0 ng/ml, respectively.
Zendelovska
Dragica
D
Institute of Preclinical and Clinical Pharmacology and Toxicology, Faculty of Medicine, Sts. Cyril and Methodius University, 50 Divizija bb, 1001 Skopje, Macedonia.
Stafilov
Trajce
T
Stefova
Marina
M
D000959:Antihypertensive Agents / Q000097:blood*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007190:Indapamide / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000959:Antihypertensive Agents; D007190:Indapamide