Genetic map of AFLP markers in the rat (Rattus norvegicus) derived from the H x B/Ipcv and B x H/Cub sets of recombinant inbred strains.
The amplified fragment length polymorphism (AFLP) technique has been used to enhance marker density in a large set of recombinant inbred strains (H x B and B x H) derived from a spontaneously hypertensive rat (SHR/OlaIpcv) and a Brown-Norway (BN.lx/Cub) inbred strain. Thirteen different primer combinations were tested and a total of 191 polymorphic bands were detected. From these polymorphic bands 89 AFLP markers could be assigned to specific chromosomes. Several of these AFLP markers were mapped to regions with low marker density, thus filling up gaps in the existing genetic map of these recombinant inbred strains. These results substantiate the value of the AFLP technology in increasing marker density in genetic maps.
Bonné
Anita C M
AC
Department of Laboratory Animal Science, Faculty of Veterinary Medicine, Graduate School of Animal Health, Utrecht University, Utrecht, The Netherlands. a.bonne@antrg.azn.nl
den Bieman
Maria G
MG
Gillissen
Gert F
GF
Kren
Vladimír
V
Krenová
Drahomíra
D
Bílá
Vlasta
V
Zídek
Václav
V
Kostka
Vlastimil
V
Musilová
Alena
A
Pravenec
Michal
M
van Zutphen
Bert F M
BF
van Lith
Hein A
HA
D000818:Animals; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D015246:Deoxyribonuclease EcoRI / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers; D008297:Male; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D051381:Rats; D011914:Rats, Inbred BN; D011918:Rats, Inbred SHR; D011919:Rats, Inbred Strains / Q000235:genetics*
D005819:Genetic Markers; D015246:Deoxyribonuclease EcoRI; D015252:Deoxyribonucleases, Type II Site-Specific; C027046:TCGA-specific type II deoxyribonucleases