title: Oxidative post-translational modification of tryptophan residues in cardiac mitochondrial proteins.
abstract: We examined the distribution of N-formylkynurenine, a product of the dioxidation of tryptophan residues in proteins, throughout the human heart mitochondrial proteome. This oxidized amino acid is associated with a distinct subset of proteins, including an over-representation of complex I subunits as well as complex V subunits and enzymes involved in redox metabolism. No relationship was observed between the tryptophan modification and methionine oxidation, a known artifact of sample handling. As the mitochondria were isolated from normal human heart tissue and not subject to any artificially induced oxidative stress, we suggest that the susceptible tryptophan residues in this group of proteins are "hot spots" for oxidation in close proximity to a source of reactive oxygen species in respiring mitochondria.
authors.affiliation: MitoKor, San Diego, California 92121, USA. taylors@mitokor.com
authors.forename: Steven W
authors.initials: SW
authors.lastname: Taylor
authors.forename: Eoin
authors.initials: E
authors.lastname: Fahy
authors.forename: James
authors.initials: J
authors.lastname: Murray
authors.forename: Roderick A
authors.initials: RA
authors.lastname: Capaldi
authors.forename: Soumitra S
authors.initials: SS
authors.lastname: Ghosh
mesh_terms: D000595:Amino Acid Sequence; D006801:Humans; D008929:Mitochondria, Heart / Q000378:metabolism*; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D011499:Protein Processing, Post-Translational; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014364:Tryptophan / Q000378:metabolism*
chemical_list: D009124:Muscle Proteins; D014364:Tryptophan