Bombinakinin M gene associated peptide, a novel bioactive peptide from skin secretions of the toad Bombina maxima.
A novel 28-amino acid peptide, termed bombinakinin-GAP, was purified and characterized from skin secretions of the toad Bombina maxima. Its primary structure was established as DMYEIKQYKTAHGRPPICAPGEQCPIWV-NH(2), in which two cysteines form a disulfide bond. A FASTA search of SWISS-PROT databank detected a 32% sequence identity between the sequences of the peptide and a segment of rat cocaine- and amphetamine-regulated transcript (CART). Intracerebroventricular (i.c.v.) administration of the peptide induced a significant decrease in food intake in rats, suggesting that it played a role in the control of feeding by brain. Analysis of its cDNA structure revealed that this peptide is coexpressed with bombinakinin M, a bradykinin-related peptide from the same toad. Bombinakinin-GAP appears to be the first example of a novel class of bioactive peptides from amphibian skin, which may be implicated in feeding behavior.
Lai
Ren
R
Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, 32 East Jiao Chang Road, Yunnan 650223, Kunming, PR China.
Liu
Hen
H
Lee
Wen Hui
WH
Zhang
Yun
Y
D000595:Amino Acid Sequence; D000818:Animals; D001001:Anura / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D004435:Eating / Q000187:drug effects; D007276:Injections, Intraventricular; D007705:Kinins / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology; D008297:Male; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012867:Skin / Q000378:metabolism*; D013997:Time Factors; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein
D018076:DNA, Complementary; D007705:Kinins; D009419:Nerve Tissue Proteins; C480300:bombinakinin M gene associated peptide, Bombina maxima; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein