Comparison of glass K3EDTA versus plastic K2EDTA blood-drawing tubes for complete blood counts, reticulocyte counts, and white blood cell differentials.
Blood-drawing tubes made from plastic containing K2EDTA as an anticoagulant are gaining widespread use in clinical hematology. We compared complete blood count parameters, reticulocyte counts, white blood cell differentials, and flagging rates obtained with Becton Dickinson Vacutainer K3EDTA glass tubes and Vacutainer K2EDTA Plus plastic tubes and found only slight discrepancies in the results obtained with the two tube types. Although some parameter values obtained with K3EDTA glass tubes were significantly lower than those obtained with K2EDTA plastic tubes, many of these differences could be explained by the known effects of the liquid K3EDTA anticoagulant. Flagging rates on an automated cell counter were identical for the two tube types. We conclude that the differences between results obtained with K3EDTA glass tubes versus K2EDTA plastic tubes are minimal and unlikely to be of any clinical significance.
Van Cott
Elizabeth M
EM
Division of Laboratory Medicine, Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA.
Lewandrowski
Kent B
KB
Patel
Svetal
S
Grzybek
Debera Y
DY
Patel
Hemali S
HS
Fletcher
Suzanne R
SR
Kratz
Alexander
A
D000925:Anticoagulants; D001772:Blood Cell Count; D001800:Blood Specimen Collection / Q000592:standards*; D004492:Edetic Acid; D004864:Equipment and Supplies / Q000592:standards; D005898:Glass; D006801:Humans; D007958:Leukocyte Count; D010969:Plastics; D017701:Reticulocyte Count
D000925:Anticoagulants; D010969:Plastics; D004492:Edetic Acid