title: Identification of AFLP markers closely linked to the rhm gene for resistance to Southern Corn Leaf Blight in maize by using bulked segregant analysis.
abstract: Southern Corn Leaf Blight (SCLB) is an important disease in warm-temperate and tropical corn-producing areas throughout the world. We applied a combination of the amplified fragment length polymorphism (AFLP) technique and bulked segregant analysis (BSA) to a large F2 population in order to identify molecular markers linked to the rhm gene for resistance to SCLB. One co-dominant AFLP marker, p7m36, was mapped to a position 1.0 cM from rhm, and we converted this marker to an STS (sequence-tagged site) marker. Combined with the previously identified agrP144, this new marker may be useful for map-based cloning of the rhm gene and marker-assisted selection for rhm.
authors.affiliation: Forage Crop Research Institute, Japan Grassland Farming and Forage Seed Association, 388-5 Higashiakata, Nishinasuno, Tochigi 329-2742, Japan. hcai@jfsass.or.jp
authors.forename: H-w
authors.initials: HW
authors.lastname: Cai
authors.forename: Z-s
authors.initials: ZS
authors.lastname: Gao
authors.forename: N
authors.initials: N
authors.lastname: Yuyama
authors.forename: N
authors.initials: N
authors.lastname: Ogawa
mesh_terms: D001483:Base Sequence; D017343:Genes, Plant; D005819:Genetic Markers; D006375:Helminthosporium; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D011110:Polymorphism, Genetic; D016415:Sequence Alignment; D003313:Zea mays / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology
chemical_list: D005819:Genetic Markers