title: Calbindin D28K is a component of the organic matrix of lizard Podarcis sicula otoconia.
abstract: The factors controlling otoconia growth are not well known but it seems that the type of proteins contained in the otoconia regulates the initiation and/or the subsequent rates of crystal growth determining the morphology and the size of the final crystal. In order to clarify the mechanism of otoconia formation and their turnover, major proteins contained in the otoconia from the maculae of the saccule, utricle and lagena of inner ear of lizard Podarcis sicula were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Coomassie staining of SDS-PAGE resulted in a major broad band of 15 kDa and four other bands of 21, 28, 45 and 97 kDa. The proteins of 15, 21, 28 and 45 kDa were separated by high-pressure liquid chromatography on a C-4-reverse-phase column and the incubation of blots with monoclonal anti-Calbindin D28K antibodies indicated that the band of 28 kDa was Calbindin D28K, a calcium-binding protein.
authors.affiliation: Department of Genetics, General and Molecular Biology, University of Naples 'Federico II', via Mezzocannone 8, 80134, Naples, Italy.
authors.forename: Marina
authors.initials: M
authors.lastname: Piscopo
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Balsamo
authors.forename: Rosalia
authors.initials: R
authors.lastname: Mutone
authors.forename: Bice
authors.initials: B
authors.lastname: Avallone
authors.forename: Francesco
authors.initials: F
authors.lastname: Marmo
mesh_terms: D000818:Animals; D015153:Blotting, Western; D064026:Calbindins; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D008116:Lizards / Q000378:metabolism*; D010037:Otolithic Membrane / Q000737:chemistry*; D012394:Rosaniline Dyes; D064030:S100 Calcium Binding Protein G / Q000032:analysis*
chemical_list: D064026:Calbindins; D012394:Rosaniline Dyes; D064030:S100 Calcium Binding Protein G; C024757:Coomassie brilliant blue R