Evaluation of three Copan viral transport systems for the recovery of cultivatable, clinical virus isolates.
Three Copan viral transport media (VTM) were compared to the BBL Viral Culturette and Micro Test M4 systems for their ability to maintain the viability of clinical strains of enterovirus, herpes simplex virus, adenovirus and parainfluenza virus. VTM were inoculated, incubated up to 72 h at both 4 degrees C and 22 degrees C, and processed for cell culture. The concentration of infectious virus in VTM was determined by standard endpoint dilution assay. All VTM maintained viable enterovirus and adenovirus for 72 h but adenovirus from M4 and Copan CVM media were detected in cell culture at least one day sooner than from other media. Only M4 and Copan CVM media supported the recognition of low-titer herpes simplex virus in cell culture within 24 h. Parainfluenza virus was consistently detected in culture within six days when maintained in M4 and two of the Copan VTM media. Overall, the Copan CVM compared favorably to the M4 in maintaining concentrations of viable virus that could be recovered and identified rapidly in routine cell culture.
Dunn
James J
JJ
ARUP Laboratories, Inc., 500 Chipeta Way, Salt Lake City, UT 84108, USA. jdunn@cookchildrens.org
Billetdeaux
Erick
E
Skodack-Jones
Lisa
L
Carroll
Karen C
KC
D000256:Adenoviridae / Q000254:growth & development; D015169:Colony Count, Microbial; D017077:Culture Media, Conditioned; D005260:Female; D006801:Humans; D008297:Male; D010222:Parainfluenza Virus 1, Human / Q000254:growth & development; D012044:Regression Analysis; D012680:Sensitivity and Specificity; D018139:Simplexvirus / Q000254:growth & development; D013048:Specimen Handling; D014773:Virology / Q000379:methods*; D014777:Virus Diseases / Q000175:diagnosis*; D014780:Viruses / Q000254:growth & development*
D017077:Culture Media, Conditioned