title: Relationship between canine mucosal and serum immunoglobulin A (IgA) concentrations: serum IgA does not assess duodenal secretory IgA.
abstract: A double-sandwich enzyme immunoassay method was developed for determination of serum immunoglobulin A (S-IgA) and mucosal secretory immunoglobulin A (sIgA) in duodenal brush samples obtained via endoscopy and the relationship between enteric mucosal sIgA, salivary sIgA and S-IgA in dogs was examined. Twenty healthy dogs underwent routine endoscopy. A brush sample from the duodenal mucosa was obtained and washed in PBS, with a serum sample being taken concurrently. A saliva sample was collected from twelve of these dogs. S-IgA and sIgA with total protein concentrations in the duodenal washings and saliva samples were determined. A significant negative correlation (r = -0.64, P = 0.0059) was found between duodenal sIgA/protein ratios and S-IgA concentrations. Saliva sIgA/protein ratios did not correlate with sIgA/protein ratios of duodenal samples. The method described here allows for direct assessment of duodenal IgA; therefore indirect measures based on serum IgA or salivary IgA can be avoided. In addition, these indirect measures appear to be poor indicators of duodenal sIgA competence in dogs.
authors.affiliation: Department of Clinical Veterinary Sciences, Faculty of Veterinary Medicine, Helsinki University, PO. Box 57, 00014, Helsinki, Finland. Minna.Rinkinen@helsinki.fi
authors.forename: Minna
authors.initials: M
authors.lastname: Rinkinen
authors.forename: Anna-Maija
authors.initials: AM
authors.lastname: Teppo
authors.forename: Jaana
authors.initials: J
authors.lastname: Harmoinen
authors.forename: Elias
authors.initials: E
authors.lastname: Westermarck
mesh_terms: D000818:Animals; D004285:Dogs; D004386:Duodenum / Q000276:immunology*; D005260:Female; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A / Q000097:blood*; D007071:Immunoglobulin A, Secretory / Q000032:analysis*; D008297:Male; D009092:Mucous Membrane / Q000276:immunology*; D012463:Saliva / Q000276:immunology
chemical_list: D007070:Immunoglobulin A; D007071:Immunoglobulin A, Secretory