title: Regulation of mouse retinol dehydrogenases and retinal dehydrogenases in hepatocyte differentiation.
abstract: Retinoic acid (RA) is produced via two sequential reactions of retinol dehydrogenases (RDHs) and retinal dehydrogenases (RALDHs). We found that primary cultured mouse hepatocytes on a single collagen gel gradually lost hepatocyte specific morphology, and that another collagen gel overlay remarkably recovered it. The levels of albumin and liver-dominant expressed RDHs expression in hepatocytes paralleled their morphological change, decreased during single collagen gel culture, and were up-regulated by sequential collagen overlay. Quite similar to the expression changes, albumin and those RDHs' mRNA expression levels increased along with liver differentiation during pre- and postnatal liver development. Our data supports that all-trans and 9-cis RA, catalyzed by the RDHs, indeed play an important role in liver differentiation and regeneration.
authors.affiliation: Department of Internal Medicine, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
authors.forename: Kengo
authors.initials: K
authors.lastname: Tomita
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Azuma
authors.forename: Sayaka
authors.initials: S
authors.lastname: Inokuchi
authors.forename: Naoto
authors.initials: N
authors.lastname: Kitamura
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nishimura
authors.forename: Shinzo
authors.initials: S
authors.lastname: Kato
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Ishii
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D002384:Catalysis; D002454:Cell Differentiation; D002478:Cells, Cultured; D003094:Collagen; D003470:Culture Media; D005782:Gels; D022781:Hepatocytes / Q000166:cytology* / Q000201:enzymology* / Q000378:metabolism; D006836:Hydro-Lyases / Q000378:metabolism / Q000502:physiology*; D008115:Liver Regeneration; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D050697:Retinal Dehydrogenase; D014212:Tretinoin / Q000378:metabolism / Q000502:physiology*
chemical_list: D003470:Culture Media; D005782:Gels; D014212:Tretinoin; D003094:Collagen; D000445:Aldehyde Oxidoreductases; D050697:Retinal Dehydrogenase; D006836:Hydro-Lyases; C100603:retinol dehydratase