[The relationship of apolipoprotein H G1025C (Try316Ser) polymorphism with stroke and its effect on plasma lipid levels in Changsha Hans].
OBJECTIVE
To investigate the relationship between G1025C (Try316Ser) polymorphism in exon 8 of apolipoprotein H (apoH) gene and stroke and to evaluate the effect of G1025C(Try316Ser) polymorphism on plasma lipid levels in Changsha Hans.


METHODS
G1025C (Try316Ser) polymorphism in apoH gene was determined by PCR-single strand conformation polymorphism analysis and DNA sequencing in 100 healthy controls, 260 patients with stroke, and 20 stroke pedigrees. Serum antiphospholipid antibody (APA) levels were tested by enzyme linked immunosorbent assay (ELISA). Plasma lipid levels were measured by routine methods.


RESULTS
No statistically significant differences were found in frequencies of genotypes and alleles of G1025C (Try316Ser) polymorphism between the controls and stroke patients. The serum levels of TG in the GC genotype of cerebral infarction patients and controls were markedly higher than those in GG genotype.


CONCLUSIONS
There was no association betweenG1025C (Try316Ser) polymorphism and stroke in Changsha Hans. G1025C (Try316Ser) polymorphism was associated with plasma lipid metabolism in Changsha Hans.
Xia
Jian
J
Department of Neuroloygy, Xiangya Hospital, Central South University, Changsha, Hunan, 410008 P. R. China. xiajianxia@21cn.com
Yang
Qidong
Q
Yang
Qiming
Q
Xu
Hongwei
H
Zhang
Le
L
D000328:Adult; D000368:Aged; D000483:Alleles; D016632:Apolipoprotein A-I / Q000097:blood; D053299:Apolipoprotein B-100; D001055:Apolipoproteins B / Q000097:blood; D001483:Base Sequence; D002543:Cerebral Hemorrhage / Q000150:complications; D002544:Cerebral Infarction / Q000150:complications; D002681:China; D002784:Cholesterol / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004252:DNA Mutational Analysis; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D008055:Lipids / Q000097:blood*; D017270:Lipoprotein(a) / Q000097:blood; D008297:Male; D008875:Middle Aged; D020125:Mutation, Missense; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D020521:Stroke / Q000097:blood / Q000209:etiology / Q000235:genetics*; D014280:Triglycerides / Q000097:blood; D053482:beta 2-Glycoprotein I
D016632:Apolipoprotein A-I; D053299:Apolipoprotein B-100; D001055:Apolipoproteins B; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D006023:Glycoproteins; D008055:Lipids; D017270:Lipoprotein(a); D014280:Triglycerides; D053482:beta 2-Glycoprotein I; D004247:DNA; D002784:Cholesterol