A Crh-specific function in carbon catabolite repression in Bacillus subtilis.
Carbon catabolite repression in Bacillus subtilis is mediated by phosphorylation of the phosphoenolpyruvate:carbohydrate phosphotransferase system intermediate HPr at a serine residue catalyzed by HPr kinase. The orthologous protein Crh functions in a similar way, but, unlike HPr, it is not functional in carbohydrate uptake. A specific function for Crh is not known. The role of HPr and Crh in repressing the citM gene encoding the Mg(2+)-citrate transporter was investigated during growth of B. subtilis on different carbon sources. In glucose minimal medium, full repression was supported by both HPr and Crh. Strains deficient in Crh or the regulatory function of HPr revealed the same repression as the wild-type strain. In contrast, in a medium containing succinate and glutamate, repression was specifically mediated via Crh. Repression was relieved in the Crh-deficient strain, but still present in the HPr mutant strain. The data are the first demonstration of a Crh-specific function in B. subtilis and suggest a role for Crh in regulation of expression during growth on substrates other than carbohydrates.
Warner
Jessica B
JB
Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, Biological Center, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands.
Lolkema
Juke S
JS
D001412:Bacillus subtilis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D001426:Bacterial Proteins; D002244:Carbon / Q000378:metabolism*; D002352:Carrier Proteins / Q000032:analysis / Q000235:genetics; D019343:Citric Acid / Q000378:metabolism; D003470:Culture Media; D015964:Gene Expression Regulation, Bacterial; D005947:Glucose / Q000378:metabolism; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000502:physiology; D010750:Phosphoproteins / Q000502:physiology*; D027981:Symporters
D001426:Bacterial Proteins; D002352:Carrier Proteins; C102671:CitM protein, Bacillus subtilis; C118042:Crh protein, Bacillus subtilis; D003470:Culture Media; D010750:Phosphoproteins; D027981:Symporters; D019343:Citric Acid; D002244:Carbon; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C030182:phosphocarrier protein HPr; D005947:Glucose