Effects of platelet factor 4 on expression of bone marrow heparan sulfate in syngenic bone marrow transplantation mice.
To explore the effects of platelet factor 4(PF4) on hematopoietic reconstitution and its mechanism in syngenic bone marrow transplantation (BMT). The syngenic BMT mice models were established. 20 and 26 h before irradiation, the mice were injected 20 micrograms/kg PF4 or PBS twice into abdominal cavity, then the donor bone marrow nuclear cells (BMNC) were transplanted. On the 7th day, spleen clone forming units (CFU-S) were counted. On the 7th, 14th and 21st day after BMT, the BMNC and megakaryoryocytes in bone marrow tissue were counted and the percentage of hematopoietic tissue and expression level of heparan sulfate in bone marrow tissue were assessed. In PF4-treated groups, the CFU-S counts on the 7th day were higher than those in BMT groups after BMT. The BMNC and megakaryoryocyte counts and the percentage of hematopoietic tissue and heparan sulfate expression level were higher than those in BMT group on the 7th, 14th and 21st day after BMT (P < 0.01 or P < 0.05). PF4 could accelerate hematopoietic reconstitution of syngenic bone marrow transplantation. The promotion of the heparan sulfate expression in bone marrow may be one of mechanisms of PF4.
Meng
Fankai
F
Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
Sun
Hanying
H
Liu
Wenli
W
Yuan
Huiling
H
Xu
Huizhen
H
Sun
Lan
L
Zhou
Yinli
Y
Ren
Tianhua
T
D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism*; D016026:Bone Marrow Transplantation; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006497:Heparan Sulfate / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010978:Platelet Factor 4 / Q000494:pharmacology*; D011837:Radiation-Protective Agents / Q000494:pharmacology; D011897:Random Allocation; D013154:Spleen / Q000166:cytology; D013234:Stem Cells; D014916:Whole-Body Irradiation
D006497:Heparan Sulfate; D010978:Platelet Factor 4; D011837:Radiation-Protective Agents