title: Constructing linkage maps in autotetraploid species using simulated annealing.
abstract: In this paper we demonstrate how molecular markers segregating in a full-sib autotetraploid mapping population can be ordered to form a linkage map using simulated annealing. This approach facilitates the examination of orders close to the optimum to see which marker placings are fixed and identify the markers whose position is less certain. A simulation study investigates the effects of population size, marker spacing, ratio of dominant to codominant markers, typing errors and missing values. The method is applied to map 30 amplified fragment length polymorphism and microsatellite markers on linkage group IV of potato.
authors.affiliation: Biomathematics and Statistics Scotland, Scottish Crop Research Institute, Invergowrie, Dundee, Scotland DD2 5DE. christine@bioss.ac.uk
authors.forename: C A
authors.initials: CA
authors.lastname: Hackett
authors.forename: B
authors.initials: B
authors.lastname: Pande
authors.forename: G J
authors.initials: GJ
authors.lastname: Bryan
mesh_terms: D002874:Chromosome Mapping; D003198:Computer Simulation; D005819:Genetic Markers; D016018:Least-Squares Analysis; D018598:Minisatellite Repeats; D010944:Plants / Q000235:genetics*; D011123:Polyploidy; D011198:Solanum tuberosum / Q000235:genetics
chemical_list: D005819:Genetic Markers