title: [The construction of cDNA expression library from the tentacles of Sagartia rosea].
abstract: A cDNA expression library of the tentacles of Sagartia rosea was constructed. The cDNA was cloned into eukaryotical expression plasmid pcDNA3. SMART protocol was used for cDNA library construction and bioinformatics analysis was carried out. 71 novel EST clones were obtained from 130 sequences in the library, of which there were 21 full-length clones, including cytolysin genes, flourescent protein, ubiquinol-cytochrome C reductase gene, elongation factor, ferritin gene riboflavin kinase gene, ribosomal protein. This provides a base for further investigating their biological activity and application.
authors.affiliation: Open Laboratory for Marine Functional Genomics, National High-Tech Department, Department of Biochemistry, College of Life Science, Zhongshan University, Guangzhou 510275, China.
authors.forename: Wen-Hua
authors.initials: WH
authors.lastname: Liu
authors.forename: Yi-Liang
authors.initials: YL
authors.lastname: Wang
authors.forename: Hui-Ping
authors.initials: HP
authors.lastname: Chen
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Jiang
authors.forename: Hong-Bin
authors.initials: HB
authors.lastname: Tu
authors.forename: Jian-Wen
authors.initials: JW
authors.lastname: Wei
authors.forename: Wen-Lie
authors.initials: WL
authors.lastname: Peng
authors.forename: An-Long
authors.initials: AL
authors.lastname: Xu
mesh_terms: D000818:Animals; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D015723:Gene Library; D012313:RNA / Q000302:isolation & purification; D012615:Sea Anemones / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D012313:RNA