title: drs (Distantly related sic) gene polymorphisms among emm12-type Streptococcus pyogenes isolates.
abstract: Twenty-eight emm12-type Streptococcus pyogenes isolates from patients with invasive and noninvasive infections or from asymptomatic carriers were genetically typed. Sequencing of drs (distantly related sic [streptococcal inhibitor of complement]) genes identified two novel alleles and revealed a polymorphism for drs similar to that of sic. No association was observed between the five different drs alleles and the five restriction patterns of the vir regulon for the isolates studied. These data suggest that drs sequencing may be useful for further differentiation of S. pyogenes isolates with emm12 and identical vir regulon restriction patterns.
authors.affiliation: Institute of Medical Microbiology and National Reference Center for Streptococci, University Hospital Aachen, 52057 Aachen, Germany. claudia.brandt@em.uni-frankfurt.de
authors.forename: Claudia M
authors.initials: CM
authors.lastname: Brandt
authors.forename: Gerhard
authors.initials: G
authors.lastname: Haase
authors.forename: Barbara
authors.initials: B
authors.lastname: Spellerberg
authors.forename: Regina
authors.initials: R
authors.lastname: Holland
authors.forename: Rudolf
authors.initials: R
authors.lastname: Lütticken
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D002352:Carrier Proteins / Q000378:metabolism*; D002353:Carrier State / Q000382:microbiology; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D018085:Regulon; D017422:Sequence Analysis, DNA; D013290:Streptococcal Infections / Q000382:microbiology; D013297:Streptococcus pyogenes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; C097739:SIC protein, Streptococcus; C014689:streptococcal M protein; C068267:mry protein, Streptococcus pyogenes