Amino propynyl benzoic acid building block in rigid spacers of divalent ligands binding to the Syk SH2 domains with equally high affinity as the natural ligand.
The construction of rigid spacers composed of amino propynyl benzoic acid building blocks is described. These spacers were used to link two phosphopeptide ligand sites towards obtaining divalent ligands with a high affinity for Syk tandem SH2 domains, which are important in signal transduction. The spacer containing two of those rigid building blocks led to a ligand which was as active as the natural ligand, indicating that this building block can be used in the design and synthesis of high affinity divalent constructs that can successfully interfere with crucial protein-protein interactions.
Dekker
Frank J
FJ
Department of Medicinal Chemistry, Utrecht Institute of Pharmaceutical Sciences, Utrecht University, The Netherlands.
de Mol
Nico J
NJ
Fischer
Marcel J E
MJ
Liskamp
Rob M J
RM
D000480:Alkynes / Q000138:chemical synthesis*; D001565:Benzoates / Q000138:chemical synthesis*; D001667:Binding, Competitive; D015195:Drug Design; D007202:Indicators and Reagents; D008024:Ligands; D020349:Surface Plasmon Resonance; D018909:src Homology Domains / Q000187:drug effects*
D000480:Alkynes; D001565:Benzoates; D007202:Indicators and Reagents; D008024:Ligands; C478318:amino propynyl benzoic acid