title: [Analysis and identification of transcriptional repression domain of ETO].
abstract: OBJECTIVE
  To further verify the transcriptional repression domains in ETO and their relationship with histone deacetylase (HDAC).
  
  
  METHODS
  Either of the ETO two zinc fingers was mutated respectively by site-directing mutagenesis. The truncation fragments of ETO were amplified by polymerase chain reaction (PCR) and cloned into eukaryotic expression plasmid pFA-CMV. By the means of DNA transfection and analysis of the transcription derived from the promoter of reporter gene, the transcriptional regulation domains of ETO was determined.
  
  
  RESULTS
  The expression plasmids carrying truncated ETO and ETO with point mutation at either zinc finger were successfully constructed. Two repression domains were found within ETO, which were located at two zinc finger motifs and 275 - 487 amino acid residues, respectively.
  
  
  CONCLUSIONS
  The transcription repression by ETO was mediated by two separated domains and closely associated with HDAC, which may be used as therapeutic target for acute myeloid leukemia M(2b).
authors.affiliation: Department of Clinical Hematology, State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood Diseases Hospital, CAMS and PUMC, Tianjin 300020, China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.forename: Chang-lai
authors.initials: CL
authors.lastname: Hao
authors.forename: Hai-yan
authors.initials: HY
authors.lastname: Xing
authors.forename: Ke-jing
authors.initials: KJ
authors.lastname: Tang
authors.forename: Jian-xiang
authors.initials: JX
authors.lastname: Wang
mesh_terms: D050676:Core Binding Factor Alpha 2 Subunit / Q000737:chemistry / Q000235:genetics; D015973:Gene Expression Regulation, Leukemic / Q000235:genetics*; D005822:Genetic Vectors; D006655:Histone Deacetylases / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D015470:Leukemia, Myeloid, Acute / Q000201:enzymology / Q000235:genetics*; D015514:Oncogene Proteins, Fusion / Q000737:chemistry / Q000235:genetics; D000075142:RUNX1 Translocation Partner 1 Protein; D014158:Transcription, Genetic; D014162:Transfection; D016335:Zinc Fingers / Q000235:genetics
chemical_list: C107844:AML1-ETO fusion protein, human; D050676:Core Binding Factor Alpha 2 Subunit; D015514:Oncogene Proteins, Fusion; D000075142:RUNX1 Translocation Partner 1 Protein; D006655:Histone Deacetylases