title: Anti-epiligrin cicatricial pemphigoid and epidermolysis bullosa acquisita: differentiation by use of indirect immunofluorescence microscopy.
abstract: Binding of autoantibodies to laminin 5 and type VII collagen causes anti-epiligrin cicatricial pemphigoid and epidermolysis bullosa acquisita, respectively. Differentiation between these two dermal-binding autoimmune bullous dermatoses is not yet possible by indirect immunofluorescence microscopy. In this study we tested whether two recently described immunofluorescence techniques, "knockout" skin substrate and fluorescent overlay antigen mapping, can differentiate between anti-epiligrin cicatricial pemphigoid and epidermolysis bullosa acquisita. A total of 10 sera were tested: 4 with antilaminin 5, and 6 with antitype VII collagen autoantibodies, as characterized by either immunoblot or immunoprecipitation analysis. Differentiation between anti-epiligrin cicatricial pemphigoid and epidermolysis bullosa acquisita was possible in all 10 sera by indirect immunofluorescence using either knockout skin substrate or fluorescent overlay antigen mapping technique.
authors.affiliation: Department of Dermatology, Groningen University Hospital, The Netherlands.
authors.forename: Robert M
authors.initials: RM
authors.lastname: Vodegel
authors.forename: Marcelus C J M
authors.initials: MC
authors.lastname: de Jong
authors.forename: Hendri H
authors.initials: HH
authors.lastname: Pas
authors.forename: Kim B
authors.initials: KB
authors.lastname: Yancey
authors.forename: Marcel F
authors.initials: MF
authors.lastname: Jonkman
mesh_terms: D001323:Autoantibodies / Q000032:analysis*; D015815:Cell Adhesion Molecules / Q000276:immunology*; D024143:Collagen Type VII / Q000276:immunology; D003937:Diagnosis, Differential; D016107:Epidermolysis Bullosa Acquisita / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D005455:Fluorescent Antibody Technique; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D008856:Microscopy, Fluorescence; D010390:Pemphigoid, Benign Mucous Membrane / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D012867:Skin / Q000276:immunology*; D000097794:Kalinin
chemical_list: D001323:Autoantibodies; D015815:Cell Adhesion Molecules; D024143:Collagen Type VII; D007074:Immunoglobulin G; D000097794:Kalinin