title: Inhibition of methanol extract from the aerial parts of Saururus chinensis on lipopolysaccharide-induced nitric oxide and prostagladin E2 production from murine macrophage RAW 264.7 cells.
abstract: As an attempt to search for bioactive natural products exerting antiinflammatory activity, we have evaluated the effects of the methanol extract from the aerial parts of Saururus chinensis (LOUR.) BAILL (Saururaceae) on lipopolysaccharide (LPS)-induced nitric oxide (NO) and prostaglandin E(2) (PGE(2)) release by the macrophage cell line RAW 264.7. Our data indicate that this extract is a potent inhibitor of NO production and it also significantly decreased PGE(2) release. Consistent with these observations, the protein and mRNA expression level of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 was inhibited by MeOH extracts of the aerial part of S. chinensis (SCM) in a dose-dependent manner. Furthermore, SCM inhibited the LPS-induced DNA binding activity of nuclear factor-kappaB (NF-kappaB), which was associated with decreased p65 protein levels in the nucleus. These results suggest that SCM inhibits LPS-induced iNOS and COX-2 expression by blocking NF-kappaB activation.
authors.affiliation: College of Pharmacy, Kyung-Hee University, Seoul, Korea.
authors.forename: Rung-Gyu
authors.initials: RG
authors.lastname: Kim
authors.forename: Kyung-Min
authors.initials: KM
authors.lastname: Shin
authors.forename: Young-Kwan
authors.initials: YK
authors.lastname: Kim
authors.forename: Hyeh-Jean
authors.initials: HJ
authors.lastname: Jeong
authors.forename: Joohun
authors.initials: J
authors.lastname: Ha
authors.forename: Jong-Won
authors.initials: JW
authors.lastname: Choi
authors.forename: Hee-Juhn
authors.initials: HJ
authors.lastname: Park
authors.forename: Kyung-Tae
authors.initials: KT
authors.lastname: Lee
mesh_terms: D000818:Animals; D002460:Cell Line; D015232:Dinoprostone / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D000432:Methanol; D051379:Mice; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D032134:Saururaceae
chemical_list: D008070:Lipopolysaccharides; D010936:Plant Extracts; D009569:Nitric Oxide; D015232:Dinoprostone; D000432:Methanol