Identification of gastrin and multiple cholecystokinin genes in teleost.
To identify the teleost gastrin, CCK/gastrin family genes were isolated from puffer and flounder. The cDNA of puffer gastrin, CCK1 and CCK2 were 678, 752 and 533bp, respectively. Puffer gastrin gene consists of three exons, in contrast, CCKs consist of four exons. Flounder gastrin mRNA (526bp) was expressed in the intestine but not in the brain. It was developed synchronously with the stomach differentiation in the larval stage. The phylogenetic analysis shows that puffer and flounder gastrin classified into the vertebrate gastrin cluster and two types of CCK were probably produced by the genome duplication occurred in teleost phylogeny.
Kurokawa
Tadahide
T
National Research Institute of Aquaculture, Fisheries Research Agency, 422-1, Nakatsuhama, Nansei, 516-0193, Mie, Japan. kurokawa@fra.affrc.go.jp
Suzuki
Tohru
T
Hashimoto
Hisashi
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002766:Cholecystokinin / Q000235:genetics* / Q000302:isolation & purification; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D019143:Evolution, Molecular; D005260:Female; D005399:Fishes / Q000235:genetics*; D005432:Flounder / Q000235:genetics; D005755:Gastrins / Q000235:genetics* / Q000302:isolation & purification; D005786:Gene Expression Regulation; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D023721:Tetraodontiformes / Q000235:genetics
D018076:DNA, Complementary; D005755:Gastrins; D012333:RNA, Messenger; D002766:Cholecystokinin