title: High-level gentamicin-resistant isolates of oral streptococci and Aerococcus from blood specimens.
abstract: Seventy-nine isolates of oral streptococci and Aerococcus obtained from blood specimens between December 1997 and January 2000 were tested for high-level gentamicin resistance. Three isolates with minimum inhibitory concentrations (MICs) of > or =1024 microg/ml were selected as potential high-level gentamicin-resistant isolates. The DNA sequence of the structural gene encoding 6'-acetyltransferase-2"-phosphotransferase in the three high-level gentamicin-resistant isolates was investigated according to the method used for Enterococcus faecalis. The results of this gene analysis showed that all the DNA sequence of Aerococcus viridans no. 70, which had the highest MIC (2048 microg/ml), matched that of E. faecalis, whereas no polymerase chain reaction (PCR) products were obtained in the other two isolates that were tested. The mechanism of gentamicin resistance in these two isolates is unknown.
authors.affiliation: Chemotherapy Division, Mitsubishi Kagaku Bio-Clinical Laboratories, Inc, 3-30-1 Shimura, Itabashi-ku, Tokyo 174-8555, Japan. mbc-ka@sa2.so-net.ne.jp
authors.forename: Intetsu
authors.initials: I
authors.lastname: Kobayashi
authors.forename: Akiko
authors.initials: A
authors.lastname: Kanayama
authors.forename: Kaoru
authors.initials: K
authors.lastname: Matsuzaki
authors.forename: Minoru
authors.initials: M
authors.lastname: Nishida
authors.forename: Noriko
authors.initials: N
authors.lastname: Nakatogawa
authors.forename: Akihiro
authors.initials: A
authors.lastname: Kaneko
mesh_terms: D000123:Acetyltransferases / Q000235:genetics; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016470:Bacteremia / Q000382:microbiology; D001769:Blood / Q000382:microbiology; D024881:Drug Resistance, Bacterial; D005839:Gentamicins / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D009055:Mouth / Q000382:microbiology; D010400:Penicillin G / Q000494:pharmacology; D010406:Penicillins / Q000494:pharmacology; D010770:Phosphotransferases / Q000235:genetics; D016133:Polymerase Chain Reaction; D013289:Streptococcaceae / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D013291:Streptococcus / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins; D010406:Penicillins; D000123:Acetyltransferases; D010770:Phosphotransferases; D010400:Penicillin G