Effects of sugar cross-linking agents and thermal treatment on the culture of fibroblasts in vitro on a (PEG/chitosan) membrane.
The influences of sugar and a thermal treatment process on the properties of a PEG/chitosan membrane were investigated in this study. With increasing thermal treatment temperature, the cross-linking density was increased and the crystal structure was distorted in the sugar-mediated polyblend. The coloration of the samples intensified from yellow or brown to dark brown during the heat exposure, indicating that a Schiff base was formed. The presence of cross-linking was determined from the FTIR spectrum, a solubility test, and water uptake. The C=N bonding shown in the FTIR spectrum indicates that a Maillard reaction occurred in the sugar-mediated polyblend. The water uptake ability of the samples tested was in the following sequence: sucrose > glucose > D-fructose, which was inverse to the trend of the cross-linking density. The compatibility of the polyblend and the sugar-mediated polyblend with fibroblasts was demonstrated. The thermal treatment has an insignificant effect on cell attachment on chitosan and the other polyblends, but different types of sugar and the cross-linking density changed the cell behavior. Consequently, chitosan/PEG6K cross-linked with sucrose at a concentration of 10 wt% and 120 degrees C thermal treatment shows potential for biomedical applications.
Wang
Jian-Wen
JW
Department of Materials Science and Engineering, National Cheng-Kung University, Tainan 70101, Taiwan, ROC. jinwen.tw@yahoo.com.tw
Hon
Min-Hsiung
MH
D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials; D002241:Carbohydrates / Q000494:pharmacology*; D018929:Cell Culture Techniques / Q000379:methods; D002686:Chitin / Q000031:analogs & derivatives*; D048271:Chitosan; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D006358:Hot Temperature; D008567:Membranes, Artificial; D051379:Mice; D008855:Microscopy, Electron, Scanning; D011092:Polyethylene Glycols; D013499:Surface Properties
D001672:Biocompatible Materials; D002241:Carbohydrates; D003432:Cross-Linking Reagents; D008567:Membranes, Artificial; D002686:Chitin; D011092:Polyethylene Glycols; D048271:Chitosan