title: Interaction between vitamin D receptor and vitamin D ligands: two-dimensional alanine scanning mutational analysis.
abstract: We present a new method to investigate the details of interaction between vitamin D nuclear receptor (VDR) and various ligands, namely a two-dimensional alanine scanning mutational analysis. In this method, the transactivation of various ligands is studied in conjunction with a series of alanine scanning mutations of the residues lining the ligand binding pocket (LBP) of VDR, and the complete set of results is profiled in a patch table. We investigated examples from four structurally diverse groups of known VDR ligands: the native vitamin D hormone and two compounds with the same side chain configuration; four 20-epi compounds; three 19-nor compounds; and two nonsecosteroids. The patch table of the results indicates characteristics of each group in terms of its interaction with 18 LBP residues. We demonstrate the validity of this approach by application to docking studies of the two nonsecosteroids.
authors.affiliation: Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10, Kanda-Surugadai, Chiyoda-ku, 101-0062, Tokyo, Japan.
authors.forename: Mihwa
authors.initials: M
authors.lastname: Choi
authors.forename: Keiko
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Toshimasa
authors.initials: T
authors.lastname: Itoh
authors.forename: Makoto
authors.initials: M
authors.lastname: Makishima
authors.forename: David J
authors.initials: DJ
authors.lastname: Mangelsdorf
authors.forename: Dino
authors.initials: D
authors.lastname: Moras
authors.forename: Hector F
authors.initials: HF
authors.lastname: DeLuca
authors.forename: Sachiko
authors.initials: S
authors.lastname: Yamada
mesh_terms: D000409:Alanine / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D015153:Blotting, Western; D019556:COS Cells; D008024:Ligands; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D016297:Mutagenesis, Site-Directed; D017354:Point Mutation; D018167:Receptors, Calcitriol / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation; D014807:Vitamin D / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D008024:Ligands; D018167:Receptors, Calcitriol; D014807:Vitamin D; D000409:Alanine