Antiplasmodial activity of alkaloid extracts from Pavetta crassipes (K. Schum) and Acanthospermum hispidum (DC), two plants used in traditional medicine in Burkina Faso.
In the course of the search for new antimalarial compounds, a study of plants traditionally used against malaria in Burkina Faso was made. An ethnobotanical study permitted the identification of plants currently used by the traditional healers and herbalists. Two plants among them were selected for further study: Pavetta crassipes (K. Schum) and Acanthospermum hispidum (DC). Alkaloid extracts of these plants were tested in vitro against two reference clones of Plasmodium falciparum: the W2 chloroquine-resistant and the D6 chloroquine-sensitive strains. Significant inhibitory activity was observed with Pavetta crassipes (IC(50)=1.23 microg/ml) and A. hispidum (IC(50)=5.02 microg/ml). Antiplasmodial activity was also evaluated against six Plasmodium falciparum isolates from children between 4 and 10 years old. The IC(50) values for the alkaloid extracts were in the range 25-670 ng/ml. These results indicated that P. falciparum wild strains were more sensitive to the alkaloid extracts than strains maintained in continuous culture. Moreover, the alkaloid extracts exhibit good in vitro antimalarial activity and weak cytotoxicity against three human cell lines (THP1, normal melanocytes, HTB-66). Isolation and structural determination are now necessary in order to precisely determine the active compounds.
Sanon
S
S
Laboratory of Pharmacology and Clinical Biochemistry of CRSBAN, UFR/SVT, University of Ouagadougou, Ouagadougou, Burkina Faso.
Azas
N
N
Gasquet
M
M
Ollivier
E
E
Mahiou
V
V
Barro
N
N
Cuzin-Ouattara
N
N
Traore
A S
AS
Esposito
F
F
Balansard
G
G
Timon-David
P
P
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry*; D002050:Burkina Faso; D002460:Cell Line; D002648:Child; D002675:Child, Preschool; D002738:Chloroquine / Q000494:pharmacology; D004351:Drug Resistance; D006801:Humans; D020128:Inhibitory Concentration 50; D016778:Malaria, Falciparum / Q000469:parasitology; D016488:Medicine, African Traditional; D021261:Parasitic Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000302:isolation & purification; D019666:Rubiaceae / Q000737:chemistry*
D000470:Alkaloids; D000962:Antimalarials; D010936:Plant Extracts; D002738:Chloroquine