title: Characterization of gene expression profiles in early bovine pregnancy using a custom cDNA microarray.
abstract: Gene expression analysis comparing nonpregnant with pregnant bovine uteri, including placenta, was performed with a custom cDNA microarray containing 1,933 independent genes. These genes were classified into six categories according to biological function, as follows: cell and tissue structural dynamics (108 genes), intercellular communication (221), intracellular metabolism (265), cell cycle and apoptosis (26), regulation of gene expression (113), expressed sequence tag (EST) and function unknown (617), and uncomplemented genes (583 clones). This array possessed bovine placental/endometrial specificity, as it included many pregnancy-specific molecules, such as pregnancy-associated glycoprotein-1 (PAGs), placental lactogen (PLs), and prolactin-related protein-1 (PRPs). A total of 77 genes were induced and 12 repressed in the placenta/endometrium. Our results point to a fundamental role for bovine placental-specific genes such as PAGs, PLs, and PRPs, in implantation and placentogenesis, and document that cDNA microarray analysis from bovine placenta/endometrium is possible and is a specific tool for monitoring genome-wide gene expression during the establishment and maintenance of pregnancy.
authors.affiliation: Laboratory of Reproductive Biology and Technology, Department of Developmental Biology, National Institute of Agrobiological Sciences, Ibaraki, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Ishiwata
authors.forename: Susumu
authors.initials: S
authors.lastname: Katsuma
authors.forename: Keiichiro
authors.initials: K
authors.lastname: Kizaki
authors.forename: Osman V
authors.initials: OV
authors.lastname: Patel
authors.forename: Hiroko
authors.initials: H
authors.lastname: Nakano
authors.forename: Toru
authors.initials: T
authors.lastname: Takahashi
authors.forename: Kei
authors.initials: K
authors.lastname: Imai
authors.forename: Akira
authors.initials: A
authors.lastname: Hirasawa
authors.forename: Satoshi
authors.initials: S
authors.lastname: Shiojima
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ikawa
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Gozoh
authors.initials: G
authors.lastname: Tsujimoto
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Izaike
authors.forename: Junichi
authors.initials: J
authors.lastname: Todoroki
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Hashizume
mesh_terms: D000818:Animals; D002417:Cattle; D018076:DNA, Complementary; D010064:Embryo Implantation / Q000502:physiology; D004622:Embryo, Mammalian / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D015723:Gene Library; D020411:Oligonucleotide Array Sequence Analysis; D010920:Placenta / Q000196:embryology; D010929:Placentation; D011247:Pregnancy
chemical_list: D018076:DNA, Complementary