Recovery of Acinetobacter radioresistens lipase by hydrophobic adsorption to n-hexadecane coated on nonwoven fabric.
A simple and clean adsorption/desorption process was proposed for recovering Acinetobacter radioresistens lipase from fermentation broth. The adsorbent used was n-hexadecane coated on a hydrophobic nonwoven fabric (NWF). n-Hexadecane has a melting point of 16-18 degrees C, and its affinity for lipase decreases markedly from liquid to solid state. Accordingly, performing the adsorption and desorption above and below, respectively, the melting point would need no extraneous materials for separation. The adsorption isotherms at various temperatures were found to follow the Langmuir model. Simulation of the batch adsorption/desorption process showed that there exists an optimal amount of adsorbent for both concentration factor and enzyme recovery; the process is restrained by equilibrium. The performance of column adsorption/desorption could also be simulated using the adsorption isotherm, and it was shown that the concentration factor was proportional to the amount of adsorbent used. The benefits of this process include easy preparation of adsorbent, low operational cost, no extraneous materials needed, negligible enzyme denaturation, high efficiency, and simple process simulation.
Wang
Hui-Min
HM
Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan.
Wu
Jau-Yann
JY
Chen
Chuh-Yung
CY
Chen
Teh-Liang
TL
D000150:Acinetobacter / Q000737:chemistry / Q000201:enzymology*; D000327:Adsorption; D000473:Alkanes / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis*; D003198:Computer Simulation; D008049:Lipase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D008567:Membranes, Artificial; D008956:Models, Chemical; D020959:Polyethylene / Q000737:chemistry; D011126:Polypropylenes / Q000737:chemistry; D013696:Temperature; D013784:Textiles; D014462:Ultrafiltration / Q000379:methods*
D000473:Alkanes; D020099:Coated Materials, Biocompatible; D008567:Membranes, Artificial; D011126:Polypropylenes; D020959:Polyethylene; D008049:Lipase; C007932:n-hexadecane