Validation of monoclonal antibody F99/97.6.1 for immunohistochemical staining of brain and tonsil in mule deer (Odocoileus hemionus) with chronic wasting disease.
A new monoclonal antibody (MAb), F99/97.6.1, that has been used to demonstrate scrapie-associated prion protein PrP(Sc) in brain and lymphoid tissues of domestic sheep with scrapie was used in an immunohistochemistry assay for diagnosis of chronic wasting disease (CWD) in mule deer (Odocoileus hemionus). The MAb F99/97.6.1 immunohistochemistry assay was evaluated in brain and tonsil tissue from 100 mule deer that had spongiform encephalopathy compatible with CWD and from 1,050 mule deer outside the CWD-endemic area. This MAb demonstrated abnormal protease-resistant prion protein (PrP(res)) in brains of all of the 100 mule deer and in 99 of the 100 tonsil samples. No immunostaining was seen in samples collected from deer outside the endemic area. MAb F99/97.6.1 demonstrated excellent properties for detection of PrP(res) in fresh, frozen, or mildly to moderately autolytic samples of brain and tonsil. This immunohistochemistry assay is a sensitive, specific, readily standardized diagnostic test for CWD in deer.
Spraker
T R
TR
Department of Pathology, College of Veterinary Medicine, Colorado State University, Fort Collins, CO 80523, USA.
O'Rourke
K I
KI
Balachandran
A
A
Zink
R R
RR
Cummings
B A
BA
Miller
M W
MW
Powers
B E
BE
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001921:Brain / Q000276:immunology* / Q000473:pathology; D003670:Deer / Q000276:immunology*; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D014066:Palatine Tonsil / Q000276:immunology* / Q000473:pathology; D018620:PrPSc Proteins / Q000276:immunology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D034081:Wasting Disease, Chronic / Q000175:diagnosis* / Q000276:immunology* / Q000473:pathology
D000911:Antibodies, Monoclonal; D018620:PrPSc Proteins