TAF7 (TAFII55) plays a role in the transcription activation by c-Jun.
c-Jun is a member of the AP-1 family of transcription factors regulating expression of specific target genes in a variety of cellular processes including proliferation, stress response, and tumorigenicity. In the present study we have analyzed the mechanism of c-Jun function as a transactivator with respect to members of the basal transcription machinery, TATA-binding protein-associated factors (TAFs). We show that one member of the family, human TAF7 (formerly TAFII55), physically interacts with c-Jun through two independent interaction domains, within the N- and C-terminal part of c-Jun. Interaction in vitro correlates with enhanced transactivation function of c-Jun in HEK293 and COS cells in the presence of increasing amounts of TAF7. TAF7 interacts preferentially with DNA-bound phosphorylated c-Jun, suggesting that TAF7 represents a novel c-Jun co-activator mediating activation of AP-1 target genes in response to extracellular signals.
Munz
Christine
C
Division of Signal Transduction and Growth Control, Deutsches Krebforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany.
Psichari
Eleni
E
Mandilis
Dimitris
D
Lavigne
Anne-Claire
AC
Spiliotaki
Maria
M
Oehler
Thomas
T
Davidson
Irwin
I
Tora
Laszlo
L
Angel
Peter
P
Pintzas
Alexander
A
D000818:Animals; D015153:Blotting, Western; D019556:COS Cells; D002460:Cell Line; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases; D008156:Luciferases / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D009841:Oligonucleotides / Q000737:chemistry; D010766:Phosphorylation; D010957:Plasmids / Q000378:metabolism; D011233:Precipitin Tests; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000378:metabolism; D035381:TATA-Binding Protein Associated Factors / Q000378:metabolism* / Q000502:physiology*; D018808:Transcription Factor AP-1 / Q000378:metabolism; D035362:Transcription Factor TFIID / Q000378:metabolism* / Q000502:physiology*; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D014162:Transfection
D009841:Oligonucleotides; D011994:Recombinant Proteins; C091537:TAF7 protein, human; D035381:TATA-Binding Protein Associated Factors; D018808:Transcription Factor AP-1; D035362:Transcription Factor TFIID; D008156:Luciferases; D005982:Glutathione Transferase; D048031:JNK Mitogen-Activated Protein Kinases; D020928:Mitogen-Activated Protein Kinases