Study of interactions between actinomycin D and DNA on carbon paste electrode (CPE) and on the hanging mercury drop (HMDE) surface.
The interaction of actinomycin (ACTD) with double stranded (ds) calf thymus DNA and single stranded (ss) DNA was studied at the carbon paste electrode surface by means of transfer voltammetry in 0.2 M phosphate buffer solution (pH 7.4). Accordingly the interaction of actinomycin (ACTD) with ds calf thymus DNA, ss DNA and supercoiled (sc) DNA was studied using hanging mercury drop electrode in 0.3 M NaCl, and 50 mM sodium phosphate buffer (pH 8.5). The different electrochemical behaviours are presented and compared in the article.
Gherghi
I Ch
ICh
Department of Chemical Engineering, Analytical Chemistry Laboratory, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Girousi
S T
ST
Voulgaropoulos
A N
AN
Tzimou-Tsitouridou
R
R
D000818:Animals; D002244:Carbon / Q000032:analysis / Q000378:metabolism*; D002417:Cattle; D004247:DNA / Q000032:analysis / Q000378:metabolism*; D003609:Dactinomycin / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D004563:Electrochemistry; D004566:Electrodes; D008628:Mercury / Q000032:analysis / Q000378:metabolism*
D003609:Dactinomycin; D002244:Carbon; D004247:DNA; D008628:Mercury