title: Cellular and humoral local immune responses in sheep experimentally infected with Oestrus ovis (Diptera: Oestridae).
abstract: Cellular and humoral local responses were investigated following repetitive artificial Oestrus ovis infections in lambs. The presence of larvae induced a huge local recruitment of either leucocytes (T and B lymphocytes, macrophages) or granulocytes (eosinophils, mast cells and globule leucocytes). This cellular response was more pronounced in the ethmoid and sinus (development sites of second and third instar larvae) than in the septum or turbinates where first instar larvae migrate. Infected lambs produced Oestrus ovis specific IgG and IgA antibodies in their mucus. This local humoral response was mainly directed against larval salivary gland antigens and not against larval digestive tract antigens. Compared to the control animals, the sinusal mucosa of infected animals was extremely thickened and the epithelium exhibited hyperplasia, metaplasia and eosinophilic exocytosis. The possible roles of these local immune responses in the regulation of O. ovis larvae populations in sheep are discussed.
authors.affiliation: UMR 959 INRA/DGER de Physiopathologie Infectieuse et Parasitaire des Ruminants, Ecole Nationale Vétérinaire, 23 chemin des Capelles, 31076 Toulouse, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Tabouret
authors.forename: Caroline
authors.initials: C
authors.lastname: Lacroux
authors.forename: Olivier
authors.initials: O
authors.lastname: Andreoletti
authors.forename: Jean Paul
authors.initials: JP
authors.lastname: Bergeaud
authors.forename: Yacob
authors.initials: Y
authors.lastname: Hailu-Tolosa
authors.forename: Hervé
authors.initials: H
authors.lastname: Hoste
authors.forename: Françoise
authors.initials: F
authors.lastname: Prevot
authors.forename: Christelle
authors.initials: C
authors.lastname: Grisez
authors.forename: Philippe
authors.initials: P
authors.lastname: Dorchies
authors.forename: Philippe
authors.initials: P
authors.lastname: Jacquiet
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D004175:Diptera / Q000276:immunology* / Q000502:physiology*; D005260:Female; D006098:Granulocytes / Q000276:immunology; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007814:Larva / Q000276:immunology / Q000502:physiology; D007962:Leukocytes / Q000276:immunology*; D008297:Male; D009198:Myiasis / Q000276:immunology* / Q000469:parasitology / Q000662:veterinary; D009297:Nasal Mucosa / Q000276:immunology; D009305:Nasopharynx / Q000276:immunology; D012469:Salivary Glands / Q000276:immunology; D034561:Sheep, Domestic / Q000276:immunology* / Q000469:parasitology*
chemical_list: D000906:Antibodies; D007070:Immunoglobulin A; D007074:Immunoglobulin G