Anti-angiogenic activity of conjugated linoleic acid on basic fibroblast growth factor-induced angiogenesis.
Conjugated linoleic acid (CLA) is a potent inhibitor of mammary carcinogenesis. Cancer cells produce various angiogenic factors which stimulate host vascular endothelial cell mitogenesis and chemotaxis for their growth and metastasis. Basic fibroblast growth factor (bFGF) is a potent angiogenic factor that is expressed in many tumors. In this study, we found that CLA decreased bFGF-induced endothelial cell proliferation and DNA synthesis in a dose-dependent manner. However, CLA did not inhibit endothelial cell migration. Furthermore, CLA showed a potent inhibitory effect on embryonic vasculogenesis and bFGF-induced angiogenesis in vivo. Collectively, these results suggest that CLA selectively inhibits the active proliferating endothelial cells induced by bFGF, which may explain its anti-carcinogenic properties in vivo.
Moon
Eun-Joung
EJ
Department of Molecular Biology, Pusan National University, Busan 609-735, Korea.
Lee
You Mie
YM
Kim
Kyu-Won
KW
D000482:Allantois / Q000098:blood supply; D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D002196:Capillaries; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002642:Chick Embryo; D003094:Collagen; D004261:DNA Replication / Q000187:drug effects; D004338:Drug Combinations; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D016222:Fibroblast Growth Factor 2 / Q000633:toxicity*; D007797:Laminin; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009389:Neovascularization, Pathologic / Q000139:chemically induced / Q000188:drug therapy*; D011509:Proteoglycans; D013936:Thymidine / Q000378:metabolism
D020533:Angiogenesis Inhibitors; D004338:Drug Combinations; D007797:Laminin; D044243:Linoleic Acids, Conjugated; D011509:Proteoglycans; D016222:Fibroblast Growth Factor 2; C059485:matrigel; D003094:Collagen; D013936:Thymidine