Addition of a propyl gallate-based procoagulant to a fibrin bandage improves hemostatic performance in a swine arterial bleeding model.
Fibrin bandages manufactured by Nycomed Austria (TC-S) were modified by the addition of Hemostyptin (HS), a proprietary platelet-activating reagent containing propyl gallate. HS was added as an additional layer to TC-S fibrin bandages and the bandages were tested for hemostatic efficacy in a swine femoral artery bleeding model. Injuries were treated with a TC-S+HS bandage preparation using HS lyophilized onto a bandage surface that was then attached to the fibrin dressing. This preparation qualitatively and quantitatively exhibited more robust blood clotting at the surgical site than the control bandages. TC-S+HS bandages were more effective than control bandages with a difference in blood loss of 251.8+/-66.5 g for TC-S bandage alone, n=12 vs. 121+/-40.7 g, n=13 for the TC-S+HS bandage, P=0.05. Bleeding times were shortened for animals treated with the HS fortified bandages and residual platelets counts in these animals were higher.
Rothwell
Stephen W
SW
Department of Blood Research, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA. stephen.rothwell@na.amedd.army.mil
Fudge
James M
JM
Chen
Wan-Keung
WK
Reid
Thomas J
TJ
Krishnamurti
Chitra
C
D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects; D016063:Blood Loss, Surgical / Q000517:prevention & control; D004195:Disease Models, Animal; D005263:Femoral Artery / Q000293:injuries; D015718:Fibrin Tissue Adhesive / Q000737:chemistry* / Q000592:standards; D006470:Hemorrhage / Q000628:therapy; D006487:Hemostasis / Q000187:drug effects; D006489:Hemostatic Techniques / Q000592:standards*; D006490:Hemostatics / Q000494:pharmacology*; D008297:Male; D009779:Occlusive Dressings / Q000592:standards*; D011435:Propyl Gallate / Q000494:pharmacology*; D013552:Swine; D014945:Wound Healing / Q000187:drug effects
D015718:Fibrin Tissue Adhesive; D006490:Hemostatics; D011435:Propyl Gallate