Effect of rubiscolin, a delta opioid peptide derived from Rubisco, on memory consolidation.
Rubiscolin-6 (YPLDLF) is a delta selective opioid peptide isolated from the enzymatic digests of ribulose bisphosphate carboxylase/oxygenase (Rubisco) from spinach leaves. In a step-through type passive avoidance test in ddY mice, rubiscolin-6 enhanced memory consolidation at doses of 3nmol/mouse after intracerebroventricular administration, and at 100mg/kg after oral administration. These doses are smaller than the optimal doses for an analgesic effect. The memory enhancing effect of rubiscolin-6 was blocked by pretreatment with the delta antagonist naltrindole, suggesting the involvement of the delta opioid receptor.
Yang
Shuzhang
S
Division of Food Bioscience and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, 611-0011, Kyoto, Japan.
Kawamura
Yukio
Y
Yoshikawa
Masaaki
M
D000284:Administration, Oral; D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D007276:Injections, Intraventricular; D008297:Male; D008568:Memory / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D009271:Naltrexone / Q000031:analogs & derivatives* / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology*; D010940:Plant Proteins / Q000737:chemistry / Q000494:pharmacology; D017465:Receptors, Opioid, delta / Q000819:agonists* / Q000037:antagonists & inhibitors; D012273:Ribulose-Bisphosphate Carboxylase / Q000494:pharmacology*
D009292:Narcotic Antagonists; D010446:Peptide Fragments; D010940:Plant Proteins; D017465:Receptors, Opioid, delta; C443992:rubiscolin 5; C443993:rubiscolin 6; D009271:Naltrexone; D012273:Ribulose-Bisphosphate Carboxylase; C055382:naltrindole