title: Genomic amplification and oncogenic properties of the KCNK9 potassium channel gene.
abstract: Representational difference analysis (RDA) of human breast cancer was used to discover a novel amplicon located at chromosomal region 8q24.3. We examined a series of breast cancer samples harboring amplification of this region and determined that KCNK9 is the sole overexpressed gene within the amplification epicenter. KCNK9 encodes a potassium channel that is amplified from 3-fold to 10-fold in 10% of breast tumors and overexpressed from 5-fold to over 100-fold in 44% of breast tumors. Overexpression of KCNK9 in cell lines promotes tumor formation and confers resistance to both hypoxia and serum deprivation, suggesting that its amplification and overexpression plays a physiologically important role in human breast cancer.
authors.affiliation: Tularik Inc, Genomics Division, 266 E Pulaski Road, Greenlawn, NY 11740, USA. dmu@tularik.com
authors.forename: David
authors.initials: D
authors.lastname: Mu
authors.forename: Liyun
authors.initials: L
authors.lastname: Chen
authors.forename: Xiping
authors.initials: X
authors.lastname: Zhang
authors.forename: Lei-Hoon
authors.initials: LH
authors.lastname: See
authors.forename: Christina M
authors.initials: CM
authors.lastname: Koch
authors.forename: Clifford
authors.initials: C
authors.lastname: Yen
authors.forename: James Jiayuan
authors.initials: JJ
authors.lastname: Tong
authors.forename: Lori
authors.initials: L
authors.lastname: Spiegel
authors.forename: Ken C Q
authors.initials: KC
authors.lastname: Nguyen
authors.forename: Allyson
authors.initials: A
authors.lastname: Servoss
authors.forename: Yue
authors.initials: Y
authors.lastname: Peng
authors.forename: Lin
authors.initials: L
authors.lastname: Pei
authors.forename: Jeffrey R
authors.initials: JR
authors.lastname: Marks
authors.forename: Scott
authors.initials: S
authors.lastname: Lowe
authors.forename: Timothy
authors.initials: T
authors.lastname: Hoey
authors.forename: Lily Yeh
authors.initials: LY
authors.lastname: Jan
authors.forename: W Richard
authors.initials: WR
authors.lastname: McCombie
authors.forename: Michael H
authors.initials: MH
authors.lastname: Wigler
authors.forename: Scott
authors.initials: S
authors.lastname: Powers
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics; D002460:Cell Line; D017690:Cell Transplantation; D002898:Chromosomes, Human, Pair 8; D015342:DNA Probes; D005260:Female; D005784:Gene Amplification; D015972:Gene Expression Regulation, Neoplastic; D015894:Genome, Human; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009857:Oncogenes; D020161:Physical Chromosome Mapping; D015221:Potassium Channels / Q000235:genetics* / Q000378:metabolism*; D016324:Sequence Tagged Sites; D014183:Transplantation, Heterologous
chemical_list: D015342:DNA Probes; D015221:Potassium Channels