Dietary lipids alter the effect of steroids on transport of glucose after intestinal resection: Part II. Signalling of the response.
OBJECTIVE
Glucocorticosteroids alter the function of the intestine. Budesonide (Bud) increases the jejunal D-glucose uptake, and this effect is prevented through a polyunsaturated fatty acid (PUFA) diet. This study was undertaken to assess the possible signalling effect of budesonide, prednisone (Pred), or dexamethasone (Dex) in animals with a 50% intestinal resection and fed chow or a diet enriched with saturated (SFA) or polyunsaturated fatty acids.


METHODS
Northern blots were performed.


RESULTS
Steroids reduced the jejunal but not the ileal expression of proglucagon. Ornithine decarboxylase (ODC) expression was reduced in the jejunum.


CONCLUSIONS
c-jun, ODC, and proglucagon may be involved in the adaptive response that occurs with steroids and variations in dietary lipids after intestinal resection.
Thiesen
Aducio L
AL
Nutrition and Metabolism Research Group, Division of Gastroenterology, Department of Medicine, University of Alberta, Edmonton, Alberta, Canada.
Tappenden
Kelly A
KA
McBurney
Mike I
MI
Clandinin
M Thomas
MT
Keelan
Monika
M
Thomson
Ben K
BK
Wild
Gary E
GE
Thomson
Alan B
AB
D000714:Anastomosis, Surgical; D000818:Animals; D019819:Budesonide / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D004041:Dietary Fats / Q000494:pharmacology*; D004790:Enzyme Induction / Q000187:drug effects; D005227:Fatty Acids / Q000494:pharmacology*; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D016758:Genes, jun / Q000187:drug effects; D005934:Glucagon / Q000096:biosynthesis / Q000235:genetics; D005938:Glucocorticoids / Q000494:pharmacology*; D005947:Glucose / Q000493:pharmacokinetics*; D007082:Ileum / Q000187:drug effects / Q000378:metabolism / Q000601:surgery*; D007408:Intestinal Absorption / Q000187:drug effects*; D007583:Jejunum / Q000187:drug effects / Q000378:metabolism / Q000601:surgery*; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000235:genetics; D009955:Ornithine Decarboxylase / Q000096:biosynthesis / Q000187:drug effects; D011241:Prednisone / Q000494:pharmacology; D052336:Proglucagon; D011498:Protein Precursors / Q000096:biosynthesis / Q000235:genetics; D016755:Proto-Oncogene Proteins c-jun / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics
D004041:Dietary Fats; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D005938:Glucocorticoids; D008562:Membrane Glycoproteins; D011498:Protein Precursors; D016755:Proto-Oncogene Proteins c-jun; C504581:Stx2 protein, rat; D019819:Budesonide; D052336:Proglucagon; D003907:Dexamethasone; D005934:Glucagon; D009955:Ornithine Decarboxylase; D005947:Glucose; D011241:Prednisone