[Screening for inhibitor of vascular endothelial growth factor from random peptide library].
OBJECTIVE
To screen for the inhibitor of vascular endothelial growth factor (VEGF) 165 from random peptide library.


METHODS
Positive phage clones were rescued after two rounds of panning and competitive elution. Its affinity activity to KDR was monitored through ELISA, immunohistochemical method, Chicken CAM assay and MTT.


RESULTS
Five specific binding positive target molecule phage clones were obtained which were able to bind to cells whose surface had high KDR, among which, clone 3 and 13 could effectively block the vascularization of the chorioallantoic membrane of chick embryo, but they were not inhibitive on the proliferation of high KDR expression cells.


CONCLUSIONS
The peptides, being the inhibitors of VEGF, may be useful in the treatment of cancers.
Wu
Jie
J
Department of Medical Research, Guangzhou PLA General Hospital, Guangzhou 510010, China.
Zhang
Hongbin
H
Wang
Jie
J
Yang
Taicheng
T
Xian
Jiang
J
Yang
Chuanhong
C
Zheng
Wenling
W
Chen
Huipeng
H
Wang
Qingming
Q
D000818:Animals; D001665:Binding Sites; D016228:Endothelial Growth Factors / Q000037:antagonists & inhibitors* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D008222:Lymphokines / Q000037:antagonists & inhibitors* / Q000378:metabolism; D019151:Peptide Library; D010455:Peptides / Q000494:pharmacology; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D016228:Endothelial Growth Factors; D036341:Intercellular Signaling Peptides and Proteins; D008222:Lymphokines; D019151:Peptide Library; D010455:Peptides; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors