title: Systemic osmotic manipulations modulate ethanol-induced taurine release: a brain microdialysis study.
abstract: In recent microdialysis studies, increased extracellular concentrations of taurine after high ethanol dose administration were identified in various rat brain regions. The mechanisms by which ethanol caused these increases in extracellular taurine concentration remained unclear but could be related to ethanol-induced cell swelling. The aim of the current study was to investigate whether changes in the body osmotic state modulate the effects of ethanol on brain extracellular taurine concentrations. In several groups of rats, brain hypoosmotic or hyperosmotic states were superimposed on acute ethanol (2.0-g/kg) injections, and extracellular taurine concentrations within the nucleus accumbens were assessed by using an intracerebral microdialysis procedure. A hypoosmotic state was obtained by systemic administration of water while hyperosmotic states were induced by intraperitoneal injections of hypertonic saline solutions (1.8% or 3.6% saline). In isoosmotic conditions, ethanol induced an immediate and significant increase in taurine microdialysate content, confirming results of previous studies. However, the effects of ethanol on taurine concentrations were modulated by osmotic manipulations. Hypoosmotic conditions significantly potentiated ethanol-induced taurine release. In contrast, ethanol-induced increases in extracellular taurine levels were attenuated by 1.8% saline injection and totally prevented by 3.6% saline administration. These results strongly argue in favor of a primary role of osmoregulation in ethanol-induced taurine release. Ethanol-induced cell swelling probably activates volume-sensitive channels, and taurine passively diffuses outside the cells along its concentration gradient.
authors.affiliation: Biologie du Comportement, Université catholique de Louvain, Place Croix du Sud 1, 1348 Louvain-la-Neuve, Belgium. equertemont@ulg.ac.be
authors.forename: Etienne
authors.initials: E
authors.lastname: Quertemont
authors.forename: Audrey
authors.initials: A
authors.lastname: Devitgh
authors.forename: Philippe
authors.initials: P
authors.lastname: De Witte
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D000431:Ethanol / Q000097:blood / Q000494:pharmacology*; D008297:Male; D017551:Microdialysis / Q000379:methods*; D009995:Osmosis / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013654:Taurine / Q000378:metabolism*
chemical_list: D013654:Taurine; D000431:Ethanol