title: The structure of lipid A of the lipopolysaccharide from Burkholderia caryophylli with a 4-amino-4-deoxy-L-arabinopyranose 1-phosphate residue exclusively in glycosidic linkage.
abstract: From the lipopolysaccharides (LPSs) of the plant-pathogenic bacterium Burkholderia caryophylli, the complete structure of lipid A has been characterized. For the first time, a 4-amino-4-deoxy-L-arabinopyranose 1-phosphate residue was proven to be exclusively linked to the reducing end of lipid A from a wild-type LPS. The LPSs of B. caryophylli were degraded by mild acetate buffer hydrolysis at pH 4.4. The obtained lipid A was analyzed as such, and also after de-O-acylation or dephosphorylation. The structure of lipid A was identified mainly by means of matrix-assisted laser desorption/ionisation mass spectrometry, and by various 1D and 2D (1)H and (13)C NMR spectroscopic measurements.
authors.affiliation: Dipartimento di Chimica Organica e Biochimica, Università degli studi di Napoli Federico II, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Molinaro
authors.forename: Buko
authors.initials: B
authors.lastname: Lindner
authors.forename: Cristina
authors.initials: C
authors.lastname: De Castro
authors.forename: Birte
authors.initials: B
authors.lastname: Nolting
authors.forename: Alba
authors.initials: A
authors.lastname: Silipo
authors.forename: Rosa
authors.initials: R
authors.lastname: Lanzetta
authors.forename: Michelangelo
authors.initials: M
authors.lastname: Parrilli
authors.forename: Otto
authors.initials: O
authors.lastname: Holst
mesh_terms: D000215:Acylation; D001089:Arabinose / Q000737:chemistry*; D019117:Burkholderia / Q000737:chemistry* / Q000378:metabolism; D002236:Carbohydrate Conformation; D006027:Glycosides / Q000737:chemistry*; D008050:Lipid A / Q000737:chemistry* / Q000378:metabolism; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010755:Organophosphates / Q000737:chemistry*; D010766:Phosphorylation; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D006027:Glycosides; D008050:Lipid A; D010755:Organophosphates; D001089:Arabinose