Members of the Arabidopsis dynamin-like gene family, ADL1, are essential for plant cytokinesis and polarized cell growth.
Polarized membrane trafficking during plant cytokinesis and cell expansion are critical for plant morphogenesis, yet very little is known about the molecular mechanisms that guide this process. Dynamin and dynamin-related proteins are large GTP binding proteins that are involved in membrane trafficking. Here, we show that two functionally redundant members of the Arabidopsis dynamin-related protein family, ADL1A and ADL1E, are essential for polar cell expansion and cell plate biogenesis. adl1A-2 adl1E-1 double mutants show defects in cell plate assembly, cell wall formation, and plasma membrane recycling. Using a functional green fluorescent protein fusion protein, we show that the distribution of ADL1A is dynamic and that the protein is localized asymmetrically to the plasma membrane of newly formed and mature root cells. We propose that ADL1-mediated membrane recycling is essential for plasma membrane formation and maintenance in plants.
Kang
Byung-Ho
BH
Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Busse
James S
JS
Bednarek
Sebastian Y
SY
D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D002455:Cell Division / Q000235:genetics / Q000502:physiology; D002462:Cell Membrane / Q000235:genetics / Q000378:metabolism; D016764:Cell Polarity / Q000235:genetics / Q000502:physiology; D022081:Cell Surface Extensions / Q000235:genetics / Q000378:metabolism; D034281:Dynamins / Q000235:genetics* / Q000378:metabolism; D035264:Flowers / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D005810:Multigene Family / Q000235:genetics; D009154:Mutation; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D012639:Seeds / Q000235:genetics / Q000254:growth & development / Q000648:ultrastructure
D029681:Arabidopsis Proteins; D005966:Glucuronidase; C475162:ADL1A protein, Arabidopsis; D034281:Dynamins