UV-B induced production of MMP-2 and MMP-9 in human corneal cells.
The purpose of this study was to determine the production of metalloproteinases (MMP) 2 and 9 following UV-B irradiation in human corneal epithelial cells and fibroblasts. Epithelial cells and fibroblasts were separated from human donor corneas and exposed to UV-B lamp irradiation for 20, 40, 80 and 120 s. Media samples were collected at 8, 24, 48 and 72 h and gelatinase A and B production was assayed by the ELISA test. Statistical significance of production was assessed by the paired t-test. Increased production of MMP-2 was found in human corneal fibroblasts in response to UV-B irradiation. A statistically significant production of MMP-2 was not observed in human corneal epithelial cells following UV-B exposure. We did not detect any increase in MMP-9 after irradiation in either epithelial cells or fibroblasts. MMP-2 is produced by the corneal fibroblasts in the acute phase after UV-B irradiation. MMP-9 is not released in vitro following UV-B irradiation damage and therefore does not directly participate in the pathophysiology of acute photokeratitis.
Koźák
I
I
Department of Ophthalmology, P.J. Safárik University in Kosice, Slovak Republic. ikozak_2000@yahoo.com
Klisenbauer
D
D
Juhás
T
T
D002478:Cells, Cultured; D003315:Cornea / Q000378:metabolism / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D019573:Epithelium, Corneal / Q000378:metabolism* / Q000528:radiation effects*; D005347:Fibroblasts / Q000378:metabolism* / Q000528:radiation effects*; D006801:Humans; D066298:In Vitro Techniques; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis*; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis*; D011829:Radiation Dosage; D014466:Ultraviolet Rays
D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9