Mineralization of phenanthrene and fluoranthene in yardwaste compost.
The purpose of the study was to evaluate the potential of phenanthrene and fluoranthene biodegradation in yardwaste compost materials. These polynuclear aromatic hydrocarbons were chosen for this work because they are relatively readily biodegradable and ubiquitous in the environment. Compost samples were incubated in biometers with 14C-labeled phenanthrene and the evolution of "4CO2 was assessed as a measure of mineralization. The '4CO2 evolution varied widely among replicate biometers, possibly as the result of (1) uneven and patchy colonization of phenanthrene-degrading microorganisms on compost particles, and (2) non-uniform dispersion of the labeled substrate spike into the yardwaste microenvironment. Mineralization of phenanthrene reached about 40%extent of 14CO2 evolution at best before leveling off, but the maximum varied from sample to sample and could be as low as 1%after three months. Active mineralization occurred at mesophilic and thermophilic temperatures. Methanol extraction was used to recover "4C from biometer samples that were spiked with "4C-labeled phenanthrene. Extraction for 24-48 h yielded 1-14% recovery of 14C, depending on the length of the preceding incubation. The low extraction yield and relatively low maximum mineralization(<40%) indicated that residual phenanthrene was sorbed and bound within the compost matrix in the biometer. Amendment ofbiometers with 0.05% Tween 80 or addition of water did not consistently enhance the mineralization. Variability in mineralization was greatly reduced in liquid samples taken from pre-enriched compost samples. Mineralization of 14C-labeled fluoranthene was negligible in biometers but could be stimulated by pre-enrichment with salicylate or naphthalene. Pre-enrichment also accelerated the mineralization of phenanthrene.
Carlstrom
Carolyn J
CJ
Department of Microbiology, The Ohio State University, 484 West 12th Avenue, Columbus, OH 43210-1292, USA.
Tuovinen
Olli H
OH
D001673:Biodegradation, Environmental; D002245:Carbon Dioxide / Q000737:chemistry; D005449:Fluorenes / Q000737:chemistry; D008372:Manure; D010616:Phenanthrenes / Q000737:chemistry*; D012988:Soil Microbiology; D012989:Soil Pollutants
D005449:Fluorenes; D008372:Manure; D010616:Phenanthrenes; D012989:Soil Pollutants; D002245:Carbon Dioxide; C007738:fluoranthene; C031181:phenanthrene