[Isolation from the environment of strains of microorganisms with glucose oxidase activity].
Strains of microorganisms (13) producing enzyme glucoseoxidase were isolated from various samples of soil. The ability to oxidase glucose in presence of oxygen is most expressed in representatives of genera Aureobasidium, Aspergillus, Penicillium. Media containing the KJ-starch indicator were used at primary step for isolation of microorganisms from the environment. The strain, identified as Aureobasidium pullulans (de Bary) Arnaud, isolated from soil had the greatest glucoseoxidase activity, remained active after lyophilization, grew on the medium, which contained low concentration of glucose and nitrate for candides. Use of its cells as a glucoseoxidase sensor control allowed to detect glucose in the liquids with concentration from 0.027 mM/l.
Smotrova
N G
NG
Dniepropetrovsk State Medical Academy, 4 Oktyabrskaya Sq., Dniepropetrovsk, 49027, Ukraine.
Kremenchutskiĭ
G N
GN
D001230:Aspergillus / Q000201:enzymology* / Q000302:isolation & purification; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism; D005612:Freeze Drying; D005947:Glucose / Q000032:analysis / Q000378:metabolism; D005949:Glucose Oxidase / Q000378:metabolism*; D007202:Indicators and Reagents / Q000378:metabolism; D003904:Mitosporic Fungi / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D009566:Nitrates / Q000032:analysis / Q000378:metabolism; D010407:Penicillium / Q000201:enzymology* / Q000302:isolation & purification; D012988:Soil Microbiology; D013045:Species Specificity; D013213:Starch / Q000378:metabolism
D003470:Culture Media; D007202:Indicators and Reagents; D009566:Nitrates; D013213:Starch; D005949:Glucose Oxidase; D005947:Glucose