title: Prodigiosin induces apoptosis of B and T cells from B-cell chronic lymphocytic leukemia.
abstract: We have previously reported that prodigiosin (2-methyl-3-pentyl-6-methoxyprodigiosene) induces apoptosis in human hematopoietic cancer cell lines with no marked toxicity in nonmalignant cell lines. In this study, we demonstrate that prodigiosin induces apoptosis of B-cell chronic lymphocytic leukemia (B-CLL) cells (n=32 patients). The dose-response for the cytotoxic effect of prodigiosin was analyzed in cells from 12 patients showing an IC(50) of 116+/-25 nM. Prodigiosin induced apoptosis of B-CLL cells through caspase activation. We also analyzed the cytotoxic effect of prodigiosin in T cells from B-CLL samples and no differences were observed with respect to leukemia cells. This is the first report showing that prodigiosin induces apoptosis in human primary cancer cells.
authors.affiliation: Department de Ciències Fisiològiques II, Unitat de Bioquímica, Facultat de Medicine, Universtitat de barcelona, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Campàs
authors.forename: M
authors.initials: M
authors.lastname: Dalmau
authors.forename: B
authors.initials: B
authors.lastname: Montaner
authors.forename: M
authors.initials: M
authors.lastname: Barragán
authors.forename: B
authors.initials: B
authors.lastname: Bellosillo
authors.forename: D
authors.initials: D
authors.lastname: Colomer
authors.forename: G
authors.initials: G
authors.lastname: Pons
authors.forename: R
authors.initials: R
authors.lastname: Pérez-Tomás
authors.forename: J
authors.initials: J
authors.lastname: Gil
mesh_terms: D017920:Antiporters / Q000187:drug effects; D017209:Apoptosis / Q000187:drug effects*; D001402:B-Lymphocytes / Q000737:chemistry / Q000187:drug effects* / Q000473:pathology; D020169:Caspases / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D017136:Ion Transport / Q000187:drug effects; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000378:metabolism / Q000473:pathology*; D009363:Neoplasm Proteins / Q000032:analysis / Q000378:metabolism; D014411:Neoplastic Stem Cells / Q000737:chemistry / Q000187:drug effects* / Q000473:pathology; D011353:Prodigiosin / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000737:chemistry / Q000187:drug effects* / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000187:drug effects; D016159:Tumor Suppressor Protein p53 / Q000032:analysis
chemical_list: D017920:Antiporters; D009363:Neoplasm Proteins; D016159:Tumor Suppressor Protein p53; C114260:hydrogen-chloride symporter; D020169:Caspases; D011353:Prodigiosin