title: [Expression of full-length hepatitis E virus structural gene using baculovirus expression system].
abstract: OBJECTIVE
  To obtain recombinant baculovirus containing full-length structural gene of HEV and to express HEV structural protein.
  
  
  METHODS
  Full-length structural gene of HEV (5147-7126 nt) was inserted into baculovirus expression vector pAcUW51. The recombinant plasmid and Baculo Gold DNA were co-transfected into insect cell line Sf9. Single plaque was picked and amplified and expression of HEV structural protein was tested by SDS-PAGE, Western blot and immunofluorescence methods.
  
  
  RESULTS
  SDS-PAGE analysis showed HEV structural protein was highly expressed; Western blot and immunofluorescence assay showed that the expression product could specifically react with HEV positive sera, confirming the protein possessing HEV specific antigenicity.
  
  
  CONCLUSIONS
  HEV structural protein was successfully expressed using baculovirus expression system.
authors.affiliation: Hepatitis Branch, Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China.
authors.forename: Mingcheng
authors.initials: M
authors.lastname: Zhang
authors.forename: Yoo
authors.initials: Y
authors.lastname: Yi
authors.forename: Chongbai
authors.initials: C
authors.lastname: Liu
authors.forename: Shengli
authors.initials: S
authors.lastname: Bi
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D002478:Cells, Cultured; D015870:Gene Expression; D005796:Genes / Q000235:genetics*; D005822:Genetic Vectors; D016752:Hepatitis E virus / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D015678:Viral Structural Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; D015678:Viral Structural Proteins