The role of Ppt/Wnt5 in regulating cell shape and movement during zebrafish gastrulation.
Wnt genes play important roles in regulating patterning and morphogenesis during vertebrate gastrulation. In zebrafish, slb/wnt11 is required for convergence and extension movements, but not cell fate specification during gastrulation. To determine if other Wnt genes functionally interact with slb/wnt11, we analysed the role of ppt/wnt5 during zebrafish gastrulation. ppt/wnt5 is maternally provided and zygotically expressed at all stages during gastrulation. The analysis of ppt mutant embryos reveals that Ppt/Wnt5 regulates cell elongation and convergent extension movements in posterior regions of the gastrula, while its function in more anterior regions is largely redundant to that of Slb/Wnt11. Frizzled-2 functions downstream of ppt/wnt5, indicating that it might act as a receptor for Ppt/Wnt5 in this process. The characterisation of the role of Ppt/Wnt5 provides insight into the functional diversity of Wnt genes in regulating vertebrate gastrulation movements.
Kilian
Beate
B
Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerasse 108, 01307 Dresden, Germany.
Mansukoski
Hannu
H
Barbosa
Filipa Carreira
FC
Ulrich
Florian
F
Tada
Masazumi
M
Heisenberg
Carl Philipp
CP
D000818:Animals; D019070:Cell Lineage; D005775:Gastrula / Q000378:metabolism / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D006023:Glycoproteins / Q000235:genetics / Q000502:physiology; D017403:In Situ Hybridization; D018613:Microscopy, Confocal; D009154:Mutation; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D010641:Phenotype; D011518:Proto-Oncogene Proteins / Q000502:physiology*; D017981:Receptors, Neurotransmitter / Q000378:metabolism; D015398:Signal Transduction; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D051153:Wnt Proteins; D000071818:Wnt-5a Protein; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000502:physiology*
D006023:Glycoproteins; D016376:Oligonucleotides, Antisense; D011518:Proto-Oncogene Proteins; D017981:Receptors, Neurotransmitter; D014157:Transcription Factors; D051153:Wnt Proteins; D000071818:Wnt-5a Protein; C092568:Wnt11 protein, zebrafish; C480282:Wnt5a protein, zebrafish; D029961:Zebrafish Proteins