Controlling the intrachain segregation on a single DNA molecule.
Intrachain segregation in single DNA molecules induced by quaternary ammonium dications was studied. By means of fluorescent and electron microscopy, it was found that variations in the chemical structure of condensing agents provide one with the opportunity to control the average amount of intrachain segregation centers on the DNA single chain. The manner of interaction between the diammonium molecules was considered to be the key factor for controlling the morphology of the partially collapsed DNA molecules.
Zinchenko
Anatoly A
AA
Graduate School of Environmental Studies and CREST, JST (Japan Science and Technology), c/o School of Informatics and Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
Sergeyev
Vladimir G
VG
Murata
Shizuaki
S
Yoshikawa
Kenichi
K
D017122:Bacteriophage T4 / Q000235:genetics; D020090:Chromosome Segregation; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics; D057927:Hydrophobic and Hydrophilic Interactions; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D009690:Nucleic Acid Conformation; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*
D004279:DNA, Viral; D000644:Quaternary Ammonium Compounds