[Effect of stimulation and depression of macrophages on development of paracetamol-induced acute toxic hepatitis in rats].
The role of macrophage (Kupffer cell) stimulation and suppression in the development of a toxic liver damage was studied in rats with acute hepatitis induced by paracetamol (acetaminophen, 1000 mg/kg). Pretreatment with carboxymethylated (1-->3)-beta-D-glucan (25 mg/kg, i.p., 48 h before paracetamol) for the macrophage stimulation or with gadolinium chloride (GdCl3, 7.5 mg/kg, i.v., 24 h before paracetamol) for the macrophage suppression has a protective effect manifested by normalization of the liver function test parameters and by a decrease in the degree of morphological changes in the liver cells. A relation between these positive effects and the TNF-alpha secretion by macrophages is discussed.
Levina
O A
OA
Laboratory of Cell Biochemistry, Institute of Physiology, Siberian Division, Russian Academy of Medical Sciences, Timakova Str., 4, Novosibirsk, 630117 Russia.
Goncharova
I A
IA
Filatova
T G
TG
Nadeev
A N
AN
Korolenko
T A
TA
Sukhenko
T G
TG
Kolesnikova
O P
OP
D000082:Acetaminophen / Q000633:toxicity*; D018712:Analgesics, Non-Narcotic / Q000633:toxicity*; D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology* / Q000503:physiopathology / Q000517:prevention & control; D005682:Gadolinium / Q000008:administration & dosage; D005936:Glucans / Q000008:administration & dosage; D007728:Kupffer Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008111:Liver Function Tests; D008262:Macrophage Activation / Q000187:drug effects; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D047071:beta-Glucans
D018712:Analgesics, Non-Narcotic; D005936:Glucans; D047071:beta-Glucans; D000082:Acetaminophen; C067858:beta-glucan, (1-3)(1-4)-; D005682:Gadolinium; C038958:gadolinium chloride