title: New PCR test that recognizes all human prototypes of enterovirus: application for clinical diagnosis.
abstract: We describe a new PCR test (Penter RT-PCR) that recognizes all 64 prototypes of enterovirus. Sixty clinical samples were analyzed in parallel with this Penter RT-PCR and previously described PCR tests: 34 and 32 samples tested positive, respectively. This assay is suitable for use in clinical diagnosis, and its ability to amplify all known serotypes makes it more useful than other consensus PCR tests.
authors.affiliation: Laboratoire de Bactériologie-Virologie, GIMAP, Faculté de Médecine, St-Etienne, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Bourlet
authors.forename: Valerie
authors.initials: V
authors.lastname: Caro
authors.forename: Sophie
authors.initials: S
authors.lastname: Minjolle
authors.forename: Isabelle
authors.initials: I
authors.lastname: Jusselin
authors.forename: Bruno
authors.initials: B
authors.lastname: Pozzetto
authors.forename: Radu
authors.initials: R
authors.lastname: Crainic
authors.forename: Ronald
authors.initials: R
authors.lastname: Colimon
mesh_terms: D004770:Enterovirus / Q000145:classification* / Q000235:genetics*; D004769:Enterovirus Infections / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012703:Serotyping