title: Experimental model to estimate intestinal viability using charge-coupled device microscopy.
abstract: BACKGROUND
  Intraoperative assessment of intestinal viability following release of strangulation remains difficult. The aim of this study was to establish clinical standards for the evaluation of intestinal viability by charge-coupled device (CCD) microscopy.
  
  
  METHODS
  A rat ileus model with strangulation for between 15 and 120 min was used. The images obtained by CCD microscopy were used to calculate maximum velocity (V (max)) ratio (ratio of V (max) of blood cell transition in the experimental segment to that in the reference segment) and S ratio (the ratio of S-the effective area of the vascular bed against the total area of the vascular bed-in the experimental segment to that in the reference segment). Rats were divided into group 1, comprising animals that survived for 7 days or more, and group 2, which consisted of animals that died within 4 days.
  
  
  RESULTS
  V (max) ratio and S ratio in group 1 were both significantly higher than those in group 2 (P < 0.01). No death occurred at a V (max) ratio of 0.76 or higher and an S ratio of 0.61 or more, while there were no survivors with a V (max) ratio of 0.54 or less and an S ratio of 0.51 or less.
  
  
  CONCLUSIONS
  V (max) ratio and S ratio could be used as indices for evaluation of intestinal viability.
authors.affiliation: First Department of Surgery, Gifu University School of Medicine, 40 Tsukasa, Japan. yasumi@cc.gifu-u.ac.jp
authors.forename: M
authors.initials: M
authors.lastname: Yasumura
authors.forename: Y
authors.initials: Y
authors.lastname: Mori
authors.forename: H
authors.initials: H
authors.lastname: Takagi
authors.forename: T
authors.initials: T
authors.lastname: Yamada
authors.forename: K
authors.initials: K
authors.lastname: Sakamoto
authors.forename: H
authors.initials: H
authors.lastname: Iwata
authors.forename: H
authors.initials: H
authors.lastname: Hirose
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity; D003251:Constriction, Pathologic / Q000473:pathology; D007077:Ileal Diseases / Q000473:pathology*; D007082:Ileum / Q000098:blood supply; D007511:Ischemia / Q000473:pathology; D008026:Ligation; D008297:Male; D008853:Microscopy / Q000379:methods*; D023421:Models, Animal; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology; D014741:Video Recording