title: Quantification and diversity of the archaeal community in a landfill site.
abstract: At a sea-based, solid waste disposal site, methanogenic organisms were quantified by molecular approaches. The samples collected for analysis were from anaerobic leachate of the landfill site. When the DNA extracted from the leachate was examined by a quantitative PCR method using domain-specific 16S rDNA primers, archaeal DNA represented 2-3% of the total extracted DNA. On the basis of cloning and sequence comparison of the archaeal PCR products, more than half of the sequences belonged to Euryarchaeota, particularly relatives of the genus Methanosaeta. The cloning analysis suggested that the majority of methane emitted from the landfill site originated from the acetate-utilizing Methanosaeta.
authors.affiliation: Department of Bioprocessing, Faculty of Agriculture, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Mori
authors.forename: Richard
authors.initials: R
authors.lastname: Sparling
authors.forename: Masahiro
authors.initials: M
authors.lastname: Hatsu
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Takamizawa
mesh_terms: D001105:Archaea / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D001673:Biodegradation, Environmental; D004275:DNA, Ribosomal / Q000032:analysis; D017753:Ecosystem; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D012037:Refuse Disposal; D017422:Sequence Analysis, DNA
chemical_list: D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S