Genetic alterations detected on chromosomes 13 and 14 in Chinese non-small cell lung carcinomas.
Genetic alterations in 28 non-small cell lung carcinoma patients were detected on chromosomes 13q and 14q with microsatellite markers by polymerase chain reaction techniques. Loss of heterozygosity of up to 50% was detected with chromosome 13 markers and of up to 37% for chromosome 14. Microsatellite instability was as high as 30% on chromosome 13 and up to 19% on chromosome 14. Accumulated mutation frequencies of up to 94 and 93% were observed for chromosomes 13 and 14, respectively. Of eight tumors displaying high mutation frequencies, 1 also carried a K-ras mutation and 4 had p53 mutations. A significant association was observed between p53 mutations and genetic instability.
Kwong
Fung Mei
FM
Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong (SAR), PR China.
Wong
Po Shan
PS
Lung
Maria Li
ML
D000328:Adult; D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D002681:China; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics*; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics*; D004252:DNA Mutational Analysis; D005260:Female; D016158:Genes, p53 / Q000235:genetics; D011905:Genes, ras / Q000235:genetics; D020022:Genetic Predisposition to Disease; D006801:Humans; D019656:Loss of Heterozygosity / Q000235:genetics; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D008875:Middle Aged; D009154:Mutation / Q000235:genetics; D009367:Neoplasm Staging