[Pre-Core mutation of HBV among 26 families with history of chronic HBV infection in Shenyang].
OBJECTIVE
To investigate the Pre-Core mutations of HBV and clinical significance among families with history of chronic HBV infection.


METHODS
The G to A mutation at nucleotide 1896 in the pre C gene of HBV DNA was detected by PCR-RFLP.


RESULTS
The results showed that the mutation rate of G to A mutation at nucleotide 1896 in the pre C gene of HBV DNA was much higher in patients (56.3%) and their family members (40.5%) than in their spouses (25.0%). While the positive rate of anti-HBs in their spouses were 26.3%. On the other hand those mutations were much higher in chronic hepatitis B patients (52.4%) and HBV carriers (44.4%) than in chronic severe hepatitis B patients (20.0%).


CONCLUSIONS
The G to A mutation at nucleotide 1896 in the pre C gene of HBV DNA could be associated with persistent HBV infection.
Li
Zhiwei
Z
Department of Infectious Disease, The 2nd Affiliated Hospital, China Medical University, Shenyang 110004, China.
Dou
Xiaoguang
X
Liu
Pei
P
D000293:Adolescent; D000328:Adult; D002648:Child; D002681:China / Q000453:epidemiology; D005260:Female; D005814:Genes, Viral / Q000235:genetics*; D006515:Hepatitis B virus / Q000235:genetics*; D019694:Hepatitis B, Chronic / Q000453:epidemiology / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D017354:Point Mutation; D012150:Polymorphism, Restriction Fragment Length