The movement protein gene is involved in the virus-specific requirement of the coat protein in cell-to-cell movement of bromoviruses.
Brome mosaic virus (BMV) requires the coat protein (CP) for cell-to-cell movement whereas Cowpea chlorotic mottle virus (CCMV), from the same genus, does not. Chimeric viruses created by exchanging the movement protein (MP) gene between the viruses can move from cell to cell. We show that interference in CP expression impaired the movement of the chimeric CCMV with the BMV MP gene but not of the chimeric BMV with the CCMV MP gene. We thus conclude that the MP gene plays a crucial role in determination of the virus-specific CP requirement in bromovirus cell-to-cell movement.
Sasaki
N
N
Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
Arimoto
M
M
Nagano
H
H
Mori
M
M
Kaido
M
M
Mise
K
K
Okuno
T
T
D000595:Amino Acid Sequence; D019177:Bromoviridae / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D036022:Capsid Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D027465:Chenopodium quinoa / Q000821:virology*; D005814:Genes, Viral; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology; D053830:Plant Viral Movement Proteins; D011523:Protoplasts / Q000821:virology; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics*; D016865:Reassortant Viruses; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics*
D036022:Capsid Proteins; D008164:Luminescent Proteins; D053830:Plant Viral Movement Proteins; D012367:RNA, Viral; D014764:Viral Proteins; D049452:Green Fluorescent Proteins