One step further towards real high-throughput functional genomics.
In a recent paper by Michiels et al. an important step was made towards genuine high throughput functional genomics. The authors produced an arrayed adenoviral library containing >120000 cDNAs isolated from human placenta. This library can be used for arrayed transduction of cell lines in phenotypic assays and to screen for genes involved in the induction of any phenotype for which a robust high-throughput assay can be designed.
Oude Elferink
Ronald
R
AMC Liver Center, Academic Medical Center S2-162, Meibergdreef 69-71, Amsterdam, The Netherlands. r.p.oude-elferink@amc.uva.nl
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D001172:Arthritis, Rheumatoid / Q000235:genetics* / Q000276:immunology*; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation / Q000276:immunology*; D015723:Gene Library; D018014:Gene Transfer Techniques; D023281:Genomics / Q000379:methods* / Q000639:trends; D006801:Humans; D008264:Macrophages / Q000276:immunology