title: Differential effects of amylin and salmon calcitonin on neuropeptide gene expression in the lateral hypothalamic area and the arcuate nucleus of the rat.
abstract: The pancreatic peptide hormone amylin (AMY) and the AMY receptor agonist salmon calcitonin (sCT) reduce short-term food intake in rats primarily by activating neurons located in the circumventricular area postrema. In the present study we analyzed the involvement of (an)orexigenic neuropeptides expressed in the lateral hypothalamic area (LHA) and in the arcuate nucleus in mediating the AMY and sCT-induced suppression of food intake. By using semiquantitative in situ hybridization 120 min after intraperitoneal injection of AMY or sCT (50 microgram/kg), orexin mRNA levels were decreased in LHA by AMY or sCT treatment. Moreover, sCT significantly suppressed the orexigenic melanin concentrating hormone in LHA, whereas mRNA levels of neuropeptide Y, cocaine and amphetamine regulated transcript, agouti-gene-related protein and proopiomelanocortin were unaffected by either treatment. In conclusion, the anorexigenic effect of AMY/sCT might be mediated by the observed reduced expression of orexigenic neuropeptides in the LHA.
authors.affiliation: Institute of Nutritional Physiology, Federal Research Centre of Nutrition, Haid und Neu Strasse 9, D-76131, Karlsruhe, Germany. stephan.barth@bfe.uni-karlsruhe.de
authors.forename: Stephan W
authors.initials: SW
authors.lastname: Barth
authors.forename: Thomas
authors.initials: T
authors.lastname: Riediger
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Lutz
authors.forename: Gerhard
authors.initials: G
authors.lastname: Rechkemmer
mesh_terms: D054369:Agouti-Related Protein; D000682:Amyloid / Q000494:pharmacology*; D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology; D001111:Arcuate Nucleus of Hypothalamus / Q000187:drug effects* / Q000378:metabolism; D001345:Autoradiography / Q000379:methods; D002116:Calcitonin / Q000494:pharmacology*; D002352:Carrier Proteins / Q000235:genetics; D004435:Eating / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects*; D007026:Hypothalamic Area, Lateral / Q000187:drug effects* / Q000378:metabolism; D007028:Hypothalamic Hormones / Q000235:genetics; D017403:In Situ Hybridization / Q000379:methods; D036341:Intercellular Signaling Peptides and Proteins; D047908:Intracellular Signaling Peptides and Proteins; D058228:Islet Amyloid Polypeptide; D008297:Male; D008543:Melanins / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009478:Neuropeptide Y / Q000235:genetics; D009479:Neuropeptides / Q000235:genetics* / Q000378:metabolism; D015345:Oligonucleotide Probes; D064446:Orexin Receptors; D000068797:Orexins; D010446:Peptide Fragments / Q000494:pharmacology; D010907:Pituitary Hormones / Q000235:genetics; D011333:Pro-Opiomelanocortin / Q000235:genetics; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D043562:Receptors, G-Protein-Coupled; D018013:Receptors, Neuropeptide; D018026:Receptors, Pancreatic Hormone / Q000037:antagonists & inhibitors; D012474:Salmon; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein
chemical_list: D054369:Agouti-Related Protein; D000682:Amyloid; D000897:Anti-Ulcer Agents; D002352:Carrier Proteins; D007028:Hypothalamic Hormones; D036341:Intercellular Signaling Peptides and Proteins; D047908:Intracellular Signaling Peptides and Proteins; D058228:Islet Amyloid Polypeptide; D008543:Melanins; D009419:Nerve Tissue Proteins; D009478:Neuropeptide Y; D009479:Neuropeptides; D015345:Oligonucleotide Probes; D064446:Orexin Receptors; D000068797:Orexins; D010446:Peptide Fragments; D010907:Pituitary Hormones; D011506:Proteins; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; D018013:Receptors, Neuropeptide; D018026:Receptors, Pancreatic Hormone; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein; D011333:Pro-Opiomelanocortin; C019463:melanin-concentrating hormone; D002116:Calcitonin