title: Secretion of acid and base equivalents by intact distal airways.
abstract: Secretion of HCO(3)(-) by airway submucosal glands is essential for normal liquid and mucus secretion. Because the liquid bathing the airway surface (ASL) is acidic, it has been proposed that the surface epithelium may acidify HCO(3)(-)-rich glandular fluid. The aim of this study was to investigate the mechanisms by which intact distal bronchi, which contain both surface and glandular epithelium, modify pH of luminal fluid. Distal bronchi were isolated from pig lungs, cannulated in a bath containing HCO(3)(-)-buffered solution, and perfused continually with an aliquot of similar, lightly buffered solution (LBS) in which NaCl replaced NaHCO(3)(-) (pH 7 with NaOH). The pH of this circulating LBS initially acidified (by 0.053 +/- 0.0053 pH units) and transepithelial potential difference (PD) depolarized. The magnitude of acidification was increased when pH(LBS) was higher. This acidification was unaffected by luminal dimethylamiloride (DMA, 100 microM) but was inhibited by 100 nM bafilomycin A(1) (by 76 +/- 13%), suggesting involvement of vacuolar-H(+) ATPase. Addition of ACh (10 microM) evoked alkalinization of luminal LBS and hyperpolarization of transepithelial PD. The alkalinization was inhibited in HCO(3)(-)-free solutions containing acetazolamide (1 mM) and by DMA and was enhanced by bumetanide (100 microM), an inhibitor of Cl(-) secretion. The hyperpolarization was unaffected by these maneuvers. The anion channel blocker 5-nitro-2-(3-phenylpropylamino)benzoate (300 microM) and combined treatment with DMA and bumetanide blocked both the alkalinization and hyperpolarization responses to ACh. These results are consistent with earlier studies showing that ACh evokes glandular secretion of HCO(3)(-) and Cl(-). Isolated distal airways thus secrete both acid and base equivalents.
authors.affiliation: Lung Membrane Transport Group, Tayside Institute of Child Health, Ninewells Hospital and Medical School, University of Dundee, United Kingdom. s.k.inglis@dundee.ac.uk
authors.forename: S K
authors.initials: SK
authors.lastname: Inglis
authors.forename: S M
authors.initials: SM
authors.lastname: Wilson
authors.forename: R E
authors.initials: RE
authors.lastname: Olver
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000136:Acid-Base Equilibrium / Q000187:drug effects / Q000502:physiology*; D000143:Acids / Q000378:metabolism; D000468:Alkalies / Q000378:metabolism; D000584:Amiloride / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects / Q000502:physiology; D001980:Bronchi / Q000378:metabolism*; D002021:Buffers; D002712:Chlorides / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D018942:Macrolides; D017693:Sodium Bicarbonate / Q000378:metabolism*; D013552:Swine
chemical_list: D000143:Acids; D000468:Alkalies; D000900:Anti-Bacterial Agents; D002021:Buffers; D002712:Chlorides; D004791:Enzyme Inhibitors; D018942:Macrolides; C039465:5-dimethylamiloride; D000584:Amiloride; C040929:bafilomycin A1; D017693:Sodium Bicarbonate; D000109:Acetylcholine