Drug-associated resistance mutations in plasma and peripheral blood mononuclear cells of human immunodeficiency virus type 1-infected patients for whom highly active antiretroviral therapy is failing.
In 32 patients for whom highly active antiretroviral therapy was failing, a good agreement between drug resistance-associated mutations in plasma and peripheral blood mononuclear cells (PBMCs) was found (k = 0.85). The mutations with the lowest agreement were 20R, 63P, and 84V in the protease gene and 184V in the reverse transcriptase gene. In eight patients, primary drug resistance mutations were detected only in PBMCs.
Sarmati
Loredana
L
Department of Public Health, University of Rome Tor Vergata, Rome, Italy.
Nicastri
Emanuele
E
Uccella
Ilaria
I
D'Ettorre
Gabriella
G
Parisi
Saverio G
SG
Palmisano
Lucia
L
Galluzzo
Clementina
C
Concia
Ercole
E
Vullo
Vincenzo
V
Vella
Stefano
S
Andreoni
Massimo
M
D000328:Adult; D023241:Antiretroviral Therapy, Highly Active; D004279:DNA, Viral / Q000097:blood; D024882:Drug Resistance, Viral / Q000235:genetics*; D015658:HIV Infections / Q000188:drug therapy* / Q000821:virology; D016333:HIV Protease / Q000235:genetics; D054303:HIV Reverse Transcriptase / Q000235:genetics; D015497:HIV-1 / Q000145:classification / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology*; D008297:Male; D008875:Middle Aged; D009154:Mutation; D017211:Treatment Failure
D004279:DNA, Viral; D054303:HIV Reverse Transcriptase; D016333:HIV Protease