Phospholipid chlorohydrins cause ATP depletion and toxicity in human myeloid cells.
Chlorohydrins of stearoyl-oleoyl phosphatidylcholine (SOPC), stearoyl-linoleoyl phosphatidylcholine, and stearoyl-arachidonyl phosphatidylcholine were incubated with cultured myeloid cells (HL60) for 24 h, and the cellular ATP level was measured using a bioluminescent assay. The chlorohydrins caused significant depletion of cellular ATP in the range 10-100 microM. The ATP depletion by the phospholipid chlorohydrins was slightly less than that of 4-hydroxy-2-nonenal, but greater than that of hexanal, trans-2-nonenal, and autoxidised palmitoyl-arachidonoyl phosphatidylcholine. SOPC chlorohydrin was also found to cause loss of viability in U937 cells, and thus phospholipid chlorohydrins could contribute to the formation of a necrotic core in advanced atherosclerotic lesions.
Dever
Gary
G
Department of Bioscience, University of Strathclyde, 204 George Street, Glasgow G1 1XW, UK.
Stewart
Laura-Jayne
LJ
Pitt
Andrew R
AR
Spickett
Corinne M
CM
D000255:Adenosine Triphosphate / Q000378:metabolism*; D002728:Chlorohydrins / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D049449:Luminescence; D010743:Phospholipids / Q000494:pharmacology*; D021241:Spectrometry, Mass, Electrospray Ionization; D020298:U937 Cells
D002728:Chlorohydrins; D010743:Phospholipids; D000255:Adenosine Triphosphate