Bacterial volatiles promote growth in Arabidopsis.
Several chemical changes in soil are associated with plant growth-promoting rhizobacteria (PGPR). Some bacterial strains directly regulate plant physiology by mimicking synthesis of plant hormones, whereas others increase mineral and nitrogen availability in the soil as a way to augment growth. Identification of bacterial chemical messengers that trigger growth promotion has been limited in part by the understanding of how plants respond to external stimuli. With an increasing appreciation of how volatile organic compounds signal plants and serve in plant defense, investigations into the role of volatile components in plant-bacterial systems now can follow. Here, we present chemical and plant-growth data showing that some PGPR release a blend of volatile components that promote growth of Arabidopsis thaliana. In particular, the volatile components 2,3-butanediol and acetoin were released exclusively from two bacterial strains that trigger the greatest level of growth promotion. Furthermore, pharmacological applications of 2,3-butanediol enhanced plant growth whereas bacterial mutants blocked in 2,3-butanediol and acetoin synthesis were devoid in this growth-promotion capacity. The demonstration that PGPR strains release different volatile blends and that plant growth is stimulated by differences in these volatile blends establishes an additional function for volatile organic compounds as signaling molecules mediating plant-microbe interactions.
Ryu
Choong-Min
CM
Department of Entomology and Plant Pathology, Auburn University, 209 Life Sciences Building, Auburn, AL 36849, USA.
Farag
Mohamed A
MA
Hu
Chia-Hui
CH
Reddy
Munagala S
MS
Wei
Han-Xun
HX
Paré
Paul W
PW
Kloepper
Joseph W
JW
D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development* / Q000382:microbiology*; D001407:Bacillus / Q000378:metabolism; D001419:Bacteria / Q000378:metabolism*; D002072:Butylene Glycols / Q000378:metabolism; D016972:Enterobacter cloacae / Q000378:metabolism; D008954:Models, Biological; D009154:Mutation; D010937:Plant Growth Regulators / Q000378:metabolism*; D011551:Pseudomonas fluorescens / Q000378:metabolism; D012706:Serratia marcescens / Q000378:metabolism; D015398:Signal Transduction; D012988:Soil Microbiology; D014835:Volatilization
D002072:Butylene Glycols; D010937:Plant Growth Regulators; C026978:2,3-butylene glycol