The barrier-to-autointegration factor is a component of functional human immunodeficiency virus type 1 preintegration complexes.
Retroviral integration in vivo is mediated by preintegration complexes (PICs) derived from infectious virions. In addition to the integrase enzyme and cDNA substrate, PICs contain a variety of viral and host cell proteins. Whereas two different cell proteins, high-mobility group protein A1 (HMGA1) and the barrier-to-autointegration factor (BAF), were identified as integration cofactors based on activities in in vitro PIC assays, only HMGA1 was previously identified as a PIC component. By using antibodies against known viral and cellular PIC components, we demonstrate here functional coimmunoprecipitation of endogenous BAF protein with human immunodeficiency virus type 1 (HIV-1) PICs. Since integrase protein and integration activity were also coimmunoprecipitated by anti-BAF antibodies, we conclude that BAF is a component of HIV-1 PICs. These data are consistent with the model that BAF functions as an integration cofactor in vivo.
Lin
Chou-Wen
CW
Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.
Engelman
Alan
A
D000818:Animals; D034961:Antigens, Nuclear / Q000737:chemistry / Q000378:metabolism; D004265:DNA Helicases; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016336:Gene Products, vpr / Q000737:chemistry / Q000378:metabolism; D019427:HIV Integrase / Q000737:chemistry / Q000378:metabolism*; D015497:HIV-1 / Q000502:physiology*; D025741:HMGA1a Protein / Q000378:metabolism; D006801:Humans; D000072200:Ku Autoantigen; D009687:Nuclear Proteins; D011233:Precipitin Tests; D011817:Rabbits; D016662:Virus Integration; D054325:vpr Gene Products, Human Immunodeficiency Virus
D034961:Antigens, Nuclear; C501959:BANF1 protein, human; D004268:DNA-Binding Proteins; D016336:Gene Products, vpr; D009687:Nuclear Proteins; D054325:vpr Gene Products, Human Immunodeficiency Virus; D025741:HMGA1a Protein; D019427:HIV Integrase; D004265:DNA Helicases; C106893:XRCC5 protein, human; C000606509:Xrcc6 protein, human; D000072200:Ku Autoantigen