Molecular evolution of Bowman-Birk type proteinase inhibitors in flowering plants.
The Bowman-Birk family (BBI) of proteinase inhibitors is probably the most studied family of plant inhibitors. We describe the primary structure and the gene expression profile of 14 putative BBIs from the sugarcane expressed sequence tag database and show how we used these newly discovered sequences together with 87 previously described BBI sequences from the GenBank database to construct phylogenetic trees for the BBI family. Phylogenetic analysis revealed that BBI-type inhibitors from monocotyledonous and dicotyledonous plants could be clearly separated into different groups, while the overall topology of the BBI tree suggests a different pattern of evolution for BBI families in flowering plants. We also found that BBI proteinase inhibitors from dicotyledonous plants were well conserved, accumulating only slight differences during their evolution. In addition, we found that BBIs from monocotyledonous plants were highly variable, indicating an interesting process of evolution based on internal gene duplications and mutation events.
Mello
Marcia O
MO
Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Av Pádua Dias, 11, CP 83, 13400-970 Piracicaba, SP, Brazil.
Tanaka
Aparecida S
AS
Silva-Filho
Marcio C
MC
D000595:Amino Acid Sequence; D015152:Blotting, Northern; D003198:Computer Simulation; D019143:Evolution, Molecular; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D019684:Magnoliopsida / Q000145:classification* / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D031786:Saccharum / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014358:Trypsin Inhibitor, Bowman-Birk Soybean / Q000145:classification* / Q000235:genetics; D014361:Trypsin Inhibitors / Q000737:chemistry* / Q000145:classification
D014358:Trypsin Inhibitor, Bowman-Birk Soybean; D014361:Trypsin Inhibitors