Synthesis of polycyclic aromatic hydrocarbon-protein conjugates for preparation and immunoassay of antibodies.
The method is described dealing with the synthesis of conjugates protein-polycyclic aromatic hydrocarbons (PAHs), highly soluble in water, stable without special stabilizers and containing the minimum quantity of cross-linked products. The reaction of protein with PAH containing an aldehyde group, has been carried out in an alkaline solution, and stabilization of the conjugate has been achieved by reduction with sodium borohydride in the presence of a compound blocking the formation of an insoluble polymeric fraction. The efficiency of synthesized conjugates for the induction and immunoassay of Abs to PAH for benzo[a]pyrene is shown.
Glushkov
Andrey N
AN
Kemerovo Scientific Center, Siberian Branch of Russian Academy of Sciences, Keremovo, Russia. dci@kemtel.ru
Kostyanko
Mikhail V
MV
Cherno
Sergey V
SV
Vasilchenko
Ilya L
IL
D000818:Animals; D000906:Antibodies / Q000032:analysis* / Q000276:immunology; D000917:Antibody Formation; D001681:Biological Assay / Q000379:methods; D011084:Polycyclic Aromatic Hydrocarbons / Q000138:chemical synthesis / Q000276:immunology* / Q000378:metabolism; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000276:immunology / Q000378:metabolism
D000906:Antibodies; D011084:Polycyclic Aromatic Hydrocarbons; D012710:Serum Albumin, Bovine