title: Antimetastatic efficacy of oral 5-FU imaged by green fluorescent protein in real time.
abstract: The effectiveness of oral 5-FU in suppressing liver metastasis was assessed in a highly-metastatic mouse model. Doses of 20 and 25 mg/kg oral 5-FU significantly suppressed primary and metastatic tumor growth (p = 0.012). These inhibitory effects were more dramatic in suppressing liver metastasis (p = 0.0). The efficacy of 5-FU was visualized by whole-body fluorescence imaging of the green fluorescent protein-expressing tumor and its subsequent metastases. Toxicity was observed only in the 30 mg/kg dose. Furthermore, we showed that the non-toxic doses of 5-FU significantly prolonged survival in these animals. These data suggest the important clinical potential of oral 5-FU.
authors.affiliation: Anticancer, Inc., 7917 Ostrow Srteat, San Diego, CA 92111, USA.
authors.forename: Jin-Wei
authors.initials: JW
authors.lastname: Wang
authors.forename: Meng
authors.initials: M
authors.lastname: Yang
authors.forename: Xiaoen
authors.initials: X
authors.lastname: Wang
authors.forename: Fang-Xian
authors.initials: FX
authors.lastname: Sun
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Li
authors.forename: Shigeo
authors.initials: S
authors.lastname: Yagi
authors.forename: Robert M
authors.initials: RM
authors.lastname: Hoffman
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005472:Fluorouracil / Q000494:pharmacology*; D049452:Green Fluorescent Proteins; D006801:Humans; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy* / Q000378:metabolism / Q000556:secondary*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008819:Mice, Nude; D023041:Xenograft Model Antitumor Assays
chemical_list: D000964:Antimetabolites, Antineoplastic; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins; D005472:Fluorouracil