[Expression of PCNA and the count of AgNOR in squamous cell carcinoma of larynx and hypopharynx].
OBJECTIVE
1. To investigate the expression of PCNA (Proliferating Cell Nuclear Antigen) and the count of AgNOR(Nucleolar Organized Region) in respectively from larynx and hypopharynx. 2. To analysis the correlation between them and the clinical stage, the histological grade, the relapse, the lymph node involving and the survival period. 3. To evaluate their value on clinical prognosis.


METHODS
Immunohischemical staining was used to detect the expression of PCNA. AgNOR was detected by using Crocker's staining. The lesion tissue and normal tissue specimens were obtained from 61 patients with squamous cell carcinoma and 9 of patients with benign tumor respectively.


RESULTS
The expression of PCNA is correlate with the count of AgNOR. Both of them were increased in patients with advance clinical stage, low different tumor, tumor relapse, lymph nod involving or short survival period (P < 0.05).


CONCLUSIONS
The positive expression rate of PCNA and the count of AgNOR may be used as prediction of tumor malignancy and prognosis in squamous cell carcinoma of larynx and hypopharynx.
Zheng
Meihua
M
Department of Otorhinolaryngology, Shandong Provincial Hospital, Jinan 250021.
Tian
Xiaodong
X
Li
Hu
H
Cheng
Yali
Y
Chen
Xiuhong
X
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000556:secondary; D005260:Female; D006801:Humans; D007012:Hypopharyngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009697:Nucleolus Organizer Region / Q000473:pathology*; D011379:Prognosis; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis*; D016622:Silver Staining
D018809:Proliferating Cell Nuclear Antigen