[Study on the molecular biology of hemorrhagic fever virus in Xinjiang].
OBJECTIVE
To explore the relationship between the structure and function at molecular level and the routes of transmission of Xinjiang hemorrhagic fever (XHF) virus.


METHODS
S genes of five XHF virus strains were cloned, sequenced and compared with that of other Crimean-Congo hemorrhagic fever virus strains.


RESULTS
It was found that S genes of the five viruses had 1,672 nuclei tides, while ORF of them including 1,449 nuclei tides and coded with a protein of 482 amino acid. The nucleotides homology of Chinese isolates (93.0%-99.5%) was obviously higher than that of any other S genes strains identified in other countries'. Phylogenetic tree showed that all Chinese isolates clustered into one branch and could be further divided into another three groups.


CONCLUSIONS
The sequential difference of S genes was not totally related to the host, areas and time of the viruses isolated.
Tang
Qing
Q
Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China. tangqing@263.net
Gao
Dianping
D
Zhao
Xiuqin
X
Han
Lei
L
Hang
Changshou
C
D005798:Genes, Bacterial; D014644:Genetic Variation; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000145:classification / Q000235:genetics*; D010802:Phylogeny