title: Regulatory roles of conserved intergenic domains in vertebrate Dlx bigene clusters.
abstract: Dlx homeobox genes of vertebrates are generally arranged as three bigene clusters on distinct chromosomes. The Dlx1/Dlx2, Dlx5/Dlx6, and Dlx3/Dlx7 clusters likely originate from duplications of an ancestral Dlx gene pair. Overlaps in expression are often observed between genes from the different clusters. To determine if the overlaps are a result of the conservation of enhancer sequences between paralogous clusters, we compared the Dlx1/2 and the Dlx5/Dlx6 intergenic regions from human, mouse, zebrafish, and from two pufferfish, Spheroides nephelus and Takifugu rubripes. Conservation between all five vertebrates is limited to four sequences, two in Dlx1/Dlx2 and two in Dlx5/Dlx6. These noncoding sequences are >75% identical over a few hundred base pairs, even in distant vertebrates. However, when compared to each other, the four intergenic sequences show a much more limited similarity. Each intergenic sequence acts as an enhancer when tested in transgenic animals. Three of them are active in the forebrain with overlapping patterns despite their limited sequence similarity. The lack of sequence similarity between paralogous intergenic regions and the high degree of sequence conservation of orthologous enhancers suggest a rapid divergence of Dlx intergenic regions early in chordate/vertebrate evolution followed by fixation of cis-acting regulatory elements.
authors.affiliation: Ottawa Health Research Institute and Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Ontario, Canada K1Y 4E9.
authors.forename: Noël
authors.initials: N
authors.lastname: Ghanem
authors.forename: Olga
authors.initials: O
authors.lastname: Jarinova
authors.forename: Angel
authors.initials: A
authors.lastname: Amores
authors.forename: Qiaoming
authors.initials: Q
authors.lastname: Long
authors.forename: Gary
authors.initials: G
authors.lastname: Hatch
authors.forename: Byung Keon
authors.initials: BK
authors.lastname: Park
authors.forename: John L R
authors.initials: JL
authors.lastname: Rubenstein
authors.forename: Marc
authors.initials: M
authors.lastname: Ekker
mesh_terms: D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D017124:Conserved Sequence / Q000235:genetics / Q000502:physiology*; D003598:Cytoskeletal Proteins; D004268:DNA-Binding Proteins / Q000235:genetics; D004742:Enhancer Elements, Genetic / Q000235:genetics; D005801:Genes, Homeobox / Q000502:physiology*; D016678:Genome; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D016548:Prosencephalon / Q000196:embryology; D017434:Protein Structure, Tertiary / Q000235:genetics; D016601:RNA-Binding Proteins; D012045:Regulatory Sequences, Nucleic Acid; D023741:Takifugu; D023721:Tetraodontiformes; D014157:Transcription Factors / Q000235:genetics*; D015027:Zebrafish; D029961:Zebrafish Proteins
chemical_list: D003598:Cytoskeletal Proteins; C100596:DLX5 protein, human; C491014:DLX6 protein, human; D004268:DNA-Binding Proteins; C088334:Distal-less homeobox proteins; C501120:Dlx5 protein, mouse; C100597:Dlx6 protein, mouse; D018398:Homeodomain Proteins; D016601:RNA-Binding Proteins; C093661:Tes protein, mouse; D014157:Transcription Factors; D029961:Zebrafish Proteins; C501122:dlx5a protein, zebrafish; C491015:dlx6a protein, zebrafish