title: Synthesis of chemotactic peptide analogs with a photoaffinity cross-linker as new probes for analysis of receptor-ligand interaction.
abstract: Formylpeptide receptors are well-characterized receptors which participate in host defense responses of neutrophils. We designed and synthesized chemotactic peptide analog with p-benzoylphenylalanine (Bpa) and biotin to probe structural and mechanistic aspects of peptide-receptor interaction. These peptides possess biological activities which were dependent upon spacer residue length of and Bpa position. The covalent photoaffinity label was detected by Streptavidine-blot, which was inhibited by the parent peptide.
authors.affiliation: Micro-space Chemistry Laboratory, AIST Kyushu, National Institute of Advanced Industrial Science and Technology, Tosu, Saga, Japan.
authors.forename: Mitsukuni
authors.initials: M
authors.lastname: Shibue
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yoshiki
authors.forename: Masaya
authors.initials: M
authors.lastname: Miyazaki
authors.forename: Ichiro
authors.initials: I
authors.lastname: Fujita
authors.forename: Satoshi
authors.initials: S
authors.lastname: Osada
authors.forename: Seiji
authors.initials: S
authors.lastname: Yasuda
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakamura
authors.forename: Yuhei
authors.initials: Y
authors.lastname: Hamasaki
authors.forename: Michio
authors.initials: M
authors.lastname: Kondo
authors.forename: Hideaki
authors.initials: H
authors.lastname: Maeda
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kodama
authors.forename: Hazime
authors.initials: H
authors.lastname: Shimizu
mesh_terms: D001710:Biotin / Q000737:chemistry; D002633:Chemotaxis / Q000502:physiology*; D015335:Molecular Probes; D010455:Peptides / Q000138:chemical synthesis* / Q000378:metabolism; D010649:Phenylalanine / Q000031:analogs & derivatives* / Q000737:chemistry; D019920:Photoaffinity Labels; D015202:Protein Engineering
chemical_list: D015335:Molecular Probes; D010455:Peptides; D019920:Photoaffinity Labels; D010649:Phenylalanine; D001710:Biotin; C053764:benzoylphenylalanine