Partial filling-micellar electrokinetic chromatography optimization studies of ibuprofen, codeine and degradation products, and coupling to mass spectrometry: part II.
We describe the use of partial filling-micellar electrokinetic chromatography-mass spectrometry (PF-MEKC-MS) on the pharmaceutical ingredients ibuprofen and codeine phosphate as well as their degradation products and impurities. The study focuses on the change of the borate buffer to the volatile ammonium acetate and the optimization of critical MS parameters. The sensitivity of the method is also evaluated. The results are compared to an existing MEKC-UV method that is used for quantitative determination of the two main substances as well as for the analysis of the degradation products. It is concluded that the PF-MEKC-MS system is suitable for separation and identification.
Stubberud
Karin
K
Analytical Development, AstraZeneca R&D Södertälje, Södertälje, Sweden. karin.stubberud@astrazeneca.com
Callmer
Karin
K
Westerlund
Douglas
D
D000085:Acetates / Q000737:chemistry; D002021:Buffers; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003061:Codeine / Q000031:analogs & derivatives / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism*; D005561:Formates / Q000737:chemistry; D007052:Ibuprofen / Q000031:analogs & derivatives / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods*; D009994:Osmolar Concentration; D012680:Sensitivity and Specificity
D000085:Acetates; D002021:Buffers; D005561:Formates; C030544:formic acid; C018824:ammonium acetate; D003061:Codeine; D007052:Ibuprofen