Inhibition of staurosporine-induced apoptosis of endothelial cells by activated protein C requires protease-activated receptor-1 and endothelial cell protein C receptor.
In a model of staurosporine-induced apoptosis using EAhy926 endothelial cells, inhibition of apoptosis by activated protein C was dose-dependent and required the enzyme's active site, implicating activated protein C-mediated proteolysis. Consistent with this implication, both protease-activated receptor-1 (PAR-1) and endothelial cell protein C receptor (EPCR) were required for the anti-apoptotic effects of activated protein C.
Mosnier
Laurent O
LO
Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Griffin
John H
JH
D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D001779:Blood Coagulation Factors / Q000378:metabolism*; D002460:Cell Line; D004730:Endothelium, Vascular / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D007700:Kinetics; D008954:Models, Biological; D011493:Protein Kinase C / Q000494:pharmacology; D044463:Receptor, PAR-1; D011956:Receptors, Cell Surface / Q000378:metabolism*; D018179:Receptors, Thrombin / Q000502:physiology*; D019311:Staurosporine / Q000494:pharmacology*
D000925:Anticoagulants; D001779:Blood Coagulation Factors; D004791:Enzyme Inhibitors; D044463:Receptor, PAR-1; D011956:Receptors, Cell Surface; D018179:Receptors, Thrombin; C091963:activated protein C receptor; D011493:Protein Kinase C; D019311:Staurosporine