Apolipoprotein E (apoE) uptake and distribution in mammalian cell lines is dependent upon source of apoE and can be monitored in living cells.
As part of investigations of the cellular uptake of apolipoprotein E (apoE) relevant to Alzheimer's disease we have found that different preparations of apoE are handled differently by cells expressing the LDL-receptor. Comparing recombinant, cellular and native apoE, complexed with different preparations of lipid we find that only cellular and native apoE enter a vesicular compartment. Some, but not all of these apoE containing vesicles are lysosomes. In order to further examine the intracellular fate of apoE we demonstrate that apoE-Enhanced green fluorescent protein chimeric protein can be taken up from medium by recipient cells and tracked within these cells for extended periods.
Ljungberg
M C
MC
Department of Neuroscience, Institute of Psychiatry, King's College London, Denmark Hill De Crespigny Park, London SE5 8AF, UK.
Asuni
A
A
Pearce
J
J
Dayanandan
R
R
März
W
W
Hoffmann
M M
MM
Bertrand
P
P
Siest
G
G
Rupniak
H T R
HT
Anderton
B H
BH
Huettinger
M
M
Lovestone
S
S
D000818:Animals; D001057:Apolipoproteins E / Q000378:metabolism*; D019556:COS Cells; D002460:Cell Line; D002522:Chlorocebus aethiops; D004926:Escherichia coli; D006801:Humans; D051379:Mice; D020033:Protein Isoforms / Q000378:metabolism; D011817:Rabbits; D011994:Recombinant Proteins / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000378:metabolism
D001057:Apolipoproteins E; D020033:Protein Isoforms; D011994:Recombinant Proteins