title: New pathogenicity marker found in the plasticity region of the Helicobacter pylori genome.
abstract: Comparison of gastric carcinoma and gastritis isolates showed the presence of genes, probably carcinoma associated (JHP947 and JHP940), that are situated in a Helicobacter pylori genome region (45 kb in J99 and 68 kb in 26695) called the "plasticity region." This region presents a great variability of DNA sequences. We investigated, by PCR, the presence of the JHP940 and JHP947 genes, as well as the presence of a third gene which seems to be associated with gastritis (HP986), on H. pylori strains isolated from 200 Brazilian patients, 79 of whom had gastric carcinomas and 53 of whom had duodenal ulcers, to confirm this association. Gastritis isolates (n = 68) were included as a control. We also evaluated if these genes were related to the virulence-associated cagA genotype. The present methodology did not permit definitive conclusions to be reached regarding the association between the JHP940 gene and gastric carcinoma or between the HP986 gene and gastritis. However, we showed that the JHP947 gene might be implicated in the development of both duodenal ulcer and gastric carcinoma. The presence of the JHP947 gene was associated with the cagA-positive genotype. The JHP947 gene is a novel virulence marker candidate of H. pylori.
authors.affiliation: Laboratoire de Bactériologie, Université Victor Segalen Bordeaux 2, France.
authors.forename: Adriana
authors.initials: A
authors.lastname: Santos
authors.forename: Dulciene Maria Magalhães
authors.initials: DM
authors.lastname: Queiroz
authors.forename: Armelle
authors.initials: A
authors.lastname: Ménard
authors.forename: Armelle
authors.initials: A
authors.lastname: Marais
authors.forename: Gifone Aguiar
authors.initials: GA
authors.lastname: Rocha
authors.forename: Celso Affonso
authors.initials: CA
authors.lastname: Oliveira
authors.forename: Ana Margarida Miguel Ferreira
authors.initials: AM
authors.lastname: Nogueira
authors.forename: Milton
authors.initials: M
authors.lastname: Uzeda
authors.forename: Francis
authors.initials: F
authors.lastname: Mégraud
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002277:Carcinoma / Q000235:genetics; D004381:Duodenal Ulcer / Q000235:genetics; D005756:Gastritis / Q000235:genetics; D005819:Genetic Markers; D014644:Genetic Variation; D016680:Genome, Bacterial; D016480:Helicobacter pylori / Q000235:genetics / Q000472:pathogenicity*; D006801:Humans; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013274:Stomach Neoplasms / Q000235:genetics; D014774:Virulence / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D005819:Genetic Markers