Patch-clamp fluorometry recording of conformational rearrangements of ion channels.
Conformational rearrangements are commonly observed in membrane proteins and constitute the molecular mechanisms through which they contribute to signal transduction. Information on structural changes in membrane proteins has mostly been inferred from functional studies. Recently, site-specific fluorescence recordings have made it possible to directly observe such molecular events in real time within the membrane environment. Here, we describe the patch-clamp fluorometry (PCF) technique that records simultaneously the local structural changes and the functional states of ion channels in isolated cell membranes. Combined with fluorescence resonance energy transfer (FRET), the technique should shed new light on ion channel activation, regulation, and interaction with other membrane proteins.
Zheng
Jie
J
Howard Hughes Medical Institute, Department of Physiology and Biophysics, University of Washington School of Medicine, Box 357290, Seattle, WA 98195, USA.
Zagotta
William N
WN
D000818:Animals; D002462:Cell Membrane / Q000737:chemistry; D036681:Fluorescence Recovery After Photobleaching / Q000379:methods; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation / Q000379:methods; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry; D007473:Ion Channels / Q000737:chemistry* / Q000378:metabolism; D008301:Maleimides / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism; D009687:Nuclear Proteins / Q000737:chemistry / Q000378:metabolism; D009865:Oocytes / Q000166:cytology; D018408:Patch-Clamp Techniques / Q000295:instrumentation / Q000379:methods*; D038761:Photobleaching; D011487:Protein Conformation; D011506:Proteins; D012815:Signal Processing, Computer-Assisted; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*; D013194:Staining and Labeling / Q000295:instrumentation / Q000379:methods; D029867:Xenopus Proteins / Q000737:chemistry / Q000378:metabolism; D014982:Xenopus laevis / Q000196:embryology
D005456:Fluorescent Dyes; D007473:Ion Channels; D008301:Maleimides; D008565:Membrane Proteins; D009687:Nuclear Proteins; D011506:Proteins; D029867:Xenopus Proteins; C074370:cysteine-rich protein, mammalian; C043592:maleimide