Progesterone receptor isoform B in the human fallopian tube and endometrium following mifepristone.
The distribution of progesterone receptor isoform B may have important clinical significance. The aim of this study was to compare the expression, localization and regulation of progesterone receptor isoform B in the human Fallopian tube and endometrium following mifepristone in a dose effective for contraception. Fertile women were treated with a single dose of 200 mg mifepristone on day luteinizing hormone (LH)+2. Biopsies were obtained from the Fallopian tube on day LH+4 to LH+6 and from the endometrium on day LH+6 to LH+8. Progesterone receptor isoform B expression was analyzed by immunohistochemistry and reverse transcriptase polymerase chain reaction. Treatment with mifepristone increased progesterone receptor isoform B concentration in epithelial and stromal cells in the Fallopian tube and also increased progesterone receptor isoform B concentration in the glandular cells of the endometrium. These results further support the hypothesis that the contraceptive effect of mifepristone when given postovulatory is primarily due to alteration of the peri-implantation milieu influencing endometrial receptivity.
Sun
Xiaoxi
X
Department of Woman and Child Health, Division for Obstetrics and Gynecology, Karolinska Hospital/Institute, S-171 76, Stockholm, Sweden.
Christow
Alexander
A
Marions
Lena
L
Gemzell-Danielsson
Kristina
K
D000328:Adult; D003267:Contraception; D003280:Contraceptives, Oral, Synthetic / Q000494:pharmacology*; D004717:Endometrium / Q000187:drug effects* / Q000378:metabolism; D005187:Fallopian Tubes / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D015735:Mifepristone / Q000494:pharmacology*; D010060:Ovulation; D011980:Receptors, Progesterone / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
D003280:Contraceptives, Oral, Synthetic; D011980:Receptors, Progesterone; C097314:progesterone receptor B; D015735:Mifepristone