Investigation of the effect of space environment on the contents of atropine and scopolamine in Datura metel by capillary zone electrophoresis.
The seeds of Datura metel were carried aboard a retrievable satellite and exposed to space environment. The effects of space environment (weightlessness and ionizing radiation) on the contents of atropine and scopolamine in D. metel were investigated by using an effective capillary zone electrophoresis (CZE) method, which employed 50 mmol/l phosphate buffer (pH 8) containing 10% (v/v) tetrahydrofuran as the running buffer. The results showed that the contents of atropine and scopolamine varied to some extent, and the earth-control group has the lowest content of atropine. However, the variation of atropine and scopolamine contents in three groups was not obvious based on t-test. At the same time, the optimization of the separation was discussed in detail and the two compounds were completely separated within 10 min with satisfactory repeatability and calibration linearity.
Bo
Tao
T
Key Laboratory of Bioorganic Chemistry and Molecular Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, PR China.
Li
Ke An
KA
Liu
Huwei
H
D001285:Atropine / Q000032:analysis*; D002138:Calibration; D032302:Datura / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000528:radiation effects; D019075:Electrophoresis, Capillary / Q000379:methods; D005118:Extraterrestrial Environment; D011839:Radiation, Ionizing; D015203:Reproducibility of Results; D012601:Scopolamine / Q000032:analysis*; D012639:Seeds; D014893:Weightlessness
D004365:Drugs, Chinese Herbal; D001285:Atropine; D012601:Scopolamine