Novel in vitro functional assays for the determination of anthrax toxin components.
The characterisation and evaluation of the UK licensed human anthrax vaccine depends on several in vivo tests that determine its safety and potency. Assays for the determination of functionally active and/or immunoreactive toxin components and S-layer proteins have been developed and applied to the characterisation of anthrax vaccine. These technologies may support production of consistent and effective vaccines, and may ultimately reduce the requirements for in vivo testing.
Hallis
B
B
Centre for Applied Microbiology and Research, Salisbury, UK. bassam.hallis@camr.org.uk
Silman
N J
NJ
Noonan
S
S
Baker
R
R
Roberts
A D G
AD
Quinn
C P
CP
Marks
T
T
Wiblin
C
C
Lloyd
G
G
Robinson
A
A
Hudson
M J
MJ
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D022122:Anthrax Vaccines; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D001408:Bacillus anthracis / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D001427:Bacterial Toxins / Q000032:analysis* / Q000276:immunology; D010450:Endopeptidases / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D066298:In Vitro Techniques; D048369:MAP Kinase Kinase 1; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D051379:Mice; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism
D022122:Anthrax Vaccines; D000942:Antigens, Bacterial; D001427:Bacterial Toxins; C030325:anthrax toxin; D017346:Protein Serine-Threonine Kinases; D048369:MAP Kinase Kinase 1; C482125:MAP2K1 protein, human; C482127:Map2k1 protein, mouse; D020929:Mitogen-Activated Protein Kinase Kinases; D010450:Endopeptidases; D000262:Adenylyl Cyclases