Assessment of genotoxicity of benzidine and its structural analogues to human lymphocytes using comet assay.
Benzidine (BZ) and its six structural analogues (2-aminobiphenyl [2-ABP], 4-aminobiphenyl [4-ABP], 3,3'-diaminobenzidine [DABZ], 3,3'-dichlorobenzidine [DCBZ] 3,3'-dimethoxybenzidine [DEBZ], and 3,3'-dimthylbenzidine [DMBZ]) were examined for DNA damage in human lymphocytes using the alkaline comet assay. All the tested compounds showed a distinct disparity in their respective DNA-damaging capacities with an order of DABZ > BZ > DCBZ > 2-ABP > DEBZ > 4-ABP > DMBZ when lymphocytes were exposed to these chemicals for 2 h. Results show that the DNA-damaging effects of these compounds had no bearing on some physicochemical parameters including oxidation potentials, the energy differences between the lowest unoccupied molecular orbital and the highest occupied molecular orbital, ionization potentials, dipole moment, and relative partition coefficient. On the other hand, the free radical scavengers, including catalase, SOD, BHT, EDTA, and histidine exerted varying degrees of inhibitory effects on the DNA damage caused by benzidine. This suggests that genotoxicity in lymphocytes caused by benzidine proceeded via a reactive oxygen species (ROS)-mediated mechanism.
Chen
Ssu-Ching
SC
Department of Medicine, China Medical College, 91 Hsueh-Shih Rd., Taichung 404, Taiwan. osycchna@ksts.seed.net.tw
Kao
Chih-Ming
CM
Huang
Mei-Han
MH
Shih
Ming-Kuei
MK
Chen
Ya-Lei
YL
Huang
Shiao-Ping
SP
Liu
Tsan-Zon
TZ
D000328:Adult; D001560:Benzidines / Q000737:chemistry / Q000633:toxicity*; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000473:pathology; D008297:Male; D009153:Mutagens / Q000737:chemistry / Q000633:toxicity*; D013329:Structure-Activity Relationship
D001560:Benzidines; D004338:Drug Combinations; D016166:Free Radical Scavengers; D009153:Mutagens; C029876:benzidine