Changes in rhinovirus protein 2C allow efficient replication in mouse cells.
Rhinovirus type 16 was found to replicate in mouse L cells that express the viral receptor, human intercellular adhesion molecule 1 (ICAM-1). However, infection of these cells at a low multiplicity of infection leads to no discernible cytopathic effect, and low virus titers are produced. A variant virus, 16/L, was isolated after alternate passage of rhinovirus 16 between HeLa and ICAM-1 L cells. Infection of mouse cells with 16/L leads to higher virus titers, increased production of RNA, and total cytopathic effect. Three amino acid changes were identified in the P2 region of virus 16/L, and the adaptation phenotype mapped to two changes in protein 2C. The characterization of a rhinovirus host range mutant will facilitate the investigation of cellular proteins required for efficient viral growth and the development of a murine model for rhinovirus infection.
Harris
Julie R
JR
Department of Microbiology, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA.
Racaniello
Vincent R
VR
D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003588:Cytopathogenic Effect, Viral; D006367:HeLa Cells; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007739:L Cells; D051379:Mice; D009154:Mutation; D012367:RNA, Viral / Q000096:biosynthesis; D012229:Rhinovirus / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity / Q000502:physiology*; D012692:Serial Passage; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014779:Virus Replication
D002352:Carrier Proteins; D012367:RNA, Viral; D017361:Viral Nonstructural Proteins; D018799:Intercellular Adhesion Molecule-1; C076023:2C protein, viral