Calbindin D28K interacts with Ran-binding protein M: identification of interacting domains by NMR spectroscopy.
Calbindin D(28K) is an EF-hand containing protein that plays a vital role in neurological function. We now show that calcium-loaded calbindin D(28K) interacts with Ran-binding protein M, a protein known to play a role in microtubule function. Using NMR methods, we show that a peptide, LASIKNR, derived from Ran-binding protein M, interacts with several regions of the calcium-loaded protein including the amino terminus and two other regions that exhibit conformational exchange on the NMR timescale. We suggest that the interaction between calbindin D(28K) and Ran-binding protein M may be important in calbindin D(28K) function.
Lutz
Ward
W
Department of Biochemistry, Research Center, Mayo Clinic and Foundation, 200 First Street SW, Rochester, MN 55905, USA.
Frank
Elena M
EM
Craig
Theodore A
TA
Thompson
Richele
R
Venters
Ronald A
RA
Kojetin
Doug
D
Cavanagh
John
J
Kumar
Rajiv
R
D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D001665:Binding Sites; D064026:Calbindins; D003598:Cytoskeletal Proteins; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000378:metabolism*; D011233:Precipitin Tests; D017434:Protein Structure, Tertiary; D064030:S100 Calcium Binding Protein G / Q000737:chemistry* / Q000378:metabolism*; D020931:ran GTP-Binding Protein / Q000737:chemistry / Q000378:metabolism*
D048868:Adaptor Proteins, Signal Transducing; D064026:Calbindins; D003598:Cytoskeletal Proteins; D009687:Nuclear Proteins; C116005:Ran binding protein 9; D064030:S100 Calcium Binding Protein G; D020931:ran GTP-Binding Protein