Dendritic cells under influence of mouse cytomegalovirus have a physiologic dual role: to initiate and to restrict T cell activation.
The aim of this study is to analyze the dynamics of the mouse cytomegalovirus (MCMV)-dendritic cell (DC) interaction. Immature and mature DCs derived from the mouse stem cell line factor-dependent cell Paterson mixed potential were infected with a recombinant MCMV expressing green fluorescent protein. Infection of immature DCs resulted in DC activation and virus production, both of which may contribute to viral dissemination. The infection of mature DCs was nonproductive and was restricted to immediate-early and early viral protein expression. During early stages of MCMV infection, mature DCs up-regulated major histocompatibility complex (MHC) and costimulatory molecules and activated autologous, but not allogeneic, naive T cells. At later times of MCMV infection, DCs prevented T cell activation by down-regulation of MHC and costimulatory molecules. Thus, DCs under the influence of MCMV have a physiologic dual role: to initiate and to restrict T cell activation. The lack of immunostimulation in allogeneic settings may explain the increased risk of MCMV morbidity after allogeneic transplantation.
Mathys
Sibylle
S
Institut für Klinische Molekularbiologie und Tumorgenetik des GSF, Genzentrum, Ludwig-Maximilians-Universität München, Germany.
Schroeder
Timm
T
Ellwart
Joachim
J
Koszinowski
Ulrich H
UH
Messerle
Martin
M
Just
Ursula
U
D000818:Animals; D002454:Cell Differentiation; D003713:Dendritic Cells / Q000276:immunology / Q000821:virology*; D049452:Green Fluorescent Proteins; D006566:Herpesviridae Infections / Q000276:immunology* / Q000821:virology; D008164:Luminescent Proteins / Q000235:genetics; D008213:Lymphocyte Activation; D008285:Major Histocompatibility Complex / Q000276:immunology; D051379:Mice; D018146:Muromegalovirus / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D011995:Recombination, Genetic; D013601:T-Lymphocytes / Q000276:immunology*; D015854:Up-Regulation; D014779:Virus Replication
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins