Progress and problems in the replacement of in vivo pharmacopoeial bioassays for hormones.
In vivo bioassays for peptide and protein hormones have, until recently, represented a major use of laboratory animals. Advances in physico-chemical methodology, particularly HPLC, have moved most of these substances into the "non-biological" category. Newer soluble regulators (interferons, cytokines, growth factors), will probably be controlled by in vitro bioassays, obviating the need for validation of alternatives. Validation of in vitro alternatives for glycoproteins remains problematic, and is the subject of current research and development.
Bristow
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National Institute for Biological Standards and Control, Potters Bar, Herts UK.
Charton
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D000826:Animal Testing Alternatives; D000818:Animals; D001681:Biological Assay / Q000379:methods*; D006728:Hormones / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D010602:Pharmacopoeias as Topic
D006728:Hormones