Characterisation of the Escherichia coli mfd promoter.
The bacterial mfd gene encodes a transcription-repair coupling factor that mediates the preferential repair of DNA damage in the template strand of active transcriptional units. In this report, the transcription start site for the Escherichia coli mfd gene was determined in vivo and in vitro, and the DNA determinants for mfd transcription by deletion and site-directed mutagenesis were defined. A canonical sigma(70)-dependent promoter, mfd P1, was responsible for the majority of mfd transcription, and a core region consisting of residues -42 to +5 was sufficient for full activity in rich and minimal media.
Stanley
Louise K
LK
Department of Biochemistry, University of Bristol, School of Medical Sciences, University Walk, BS8 1TD, Bristol, UK.
Savery
Nigel J
NJ
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D014157:Transcription Factors; C072103:transcription repair coupling factor protein, Bacteria