TNF-alpha and gamma-irradiation induced activation of the Salmonella typhimurium reproduction in the organs of infected animals.
The goal of the current work is to determine the role of the TNF-alpha on the activation of the bacterial growth in an in vivo system. In order to reach this goal we studied the dynamics of the reproduction of vegetative forms of Salmonella and the recultivation of non-cultivating forms of Salmonella in infected animals. Experiments have been conducted both on animals that had been injected with exogenous TNF together with bacterial suspension and on animals that had been exposed to gamma-radiation before infection, since it is known that irradiation increases the secretion of TNF. We demonstrate that in all cases the increase in the level of TNF-alpha in animals leads to the activation of the Salmonella growth. Moreover, in this paper we present the data obtained by the method of molecular display on the identification of genes that are highly expressed in the Salmonella cells cultivated in vitro in the presence of TNF.
Romanova
Yulia M
YM
N.F. Gamaleya Institute for Epidemiology and Microbiology, Russian Academy of Medical Sciences, Gamaleya str. 18, Moscow 123098, Russia. jmromanova@mail.ru
Scheglovitova
Olga N
ON
Boshnakov
Rumen H
RH
Alekseeva
Natalia V
NV
Stepanova
Tatyana V
TV
Tomova
Alexandra S
AS
Gintsburg
Alexander L
AL
D000818:Animals; D005720:Gamma Rays / Q000009:adverse effects; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects / Q000528:radiation effects; D051379:Mice; D008807:Mice, Inbred BALB C; D009928:Organ Specificity; D012481:Salmonella Infections, Animal / Q000235:genetics / Q000378:metabolism / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000254:growth & development* / Q000528:radiation effects; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D014409:Tumor Necrosis Factor-alpha