title: Comparison of pencil-, fan-, and cone-beam dual X-ray absorptiometers for evaluation of bone mineral content in excised rat bone.
abstract: The aim of the present study was to assess the reproducibility and accuracy of measurements done on excised rat bone with three different generations of densitometers: Hologic QDR2000 pencil beam, Hologic QDR4500 fan beam, and Lunar PIXImus cone beam. The coefficients of variation for repeated measurements of bone mineral content (BMC) were 0.62 and 0.85% for pencil beam, 1.73 and 3.59% for fan beam, and 0.70 and 1.52% for cone beam for femur and tibia, respectively. BMC and ash weight were linearly correlated: 0.998 for pencil, 0.984 for fan, and 0.995 for cone beam. However, the three densitometers overestimated BMC by 10.9, 12.6, and 3.1%, respectively, and the overestimation was found to be dependent on the net BMC. The highest coefficient of correlation was found between BMC measurements from pencil and cone beam (r = 0.995). Data from cone-beam DXA were, respectively, 8.8 and 9.2% lower than those from penciland fan-beam DXA. We conclude that the three DXA instruments precisely and accurately measure BMC in excised rat bone; however, DXA overestimates BMC with a dependence on the bone ash weight. This dependence was less pronounced with the cone-beam technology.
authors.affiliation: LHEA-GEROM, Laboratoire d'Histologie-Embryologie, CHU & Faculté de Médecine, Angers Cédex, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Libouban
authors.forename: Yannick
authors.initials: Y
authors.lastname: Simon
authors.forename: Caroline
authors.initials: C
authors.lastname: Silve
authors.forename: Erick
authors.initials: E
authors.lastname: Legrand
authors.forename: Michel Félix
authors.initials: MF
authors.lastname: Baslé
authors.forename: Maurice
authors.initials: M
authors.lastname: Audran
authors.forename: Daniel
authors.initials: D
authors.lastname: Chappard
mesh_terms: D015502:Absorptiometry, Photon / Q000295:instrumentation* / Q000379:methods; D000818:Animals; D015519:Bone Density; D005269:Femur / Q000502:physiology; D008297:Male; D008903:Minerals; D051381:Rats; D013977:Tibia / Q000502:physiology
chemical_list: D008903:Minerals; C028705:bone ash