Relationship between canine mucosal and serum immunoglobulin A (IgA) concentrations: serum IgA does not assess duodenal secretory IgA.
A double-sandwich enzyme immunoassay method was developed for determination of serum immunoglobulin A (S-IgA) and mucosal secretory immunoglobulin A (sIgA) in duodenal brush samples obtained via endoscopy and the relationship between enteric mucosal sIgA, salivary sIgA and S-IgA in dogs was examined. Twenty healthy dogs underwent routine endoscopy. A brush sample from the duodenal mucosa was obtained and washed in PBS, with a serum sample being taken concurrently. A saliva sample was collected from twelve of these dogs. S-IgA and sIgA with total protein concentrations in the duodenal washings and saliva samples were determined. A significant negative correlation (r = -0.64, P = 0.0059) was found between duodenal sIgA/protein ratios and S-IgA concentrations. Saliva sIgA/protein ratios did not correlate with sIgA/protein ratios of duodenal samples. The method described here allows for direct assessment of duodenal IgA; therefore indirect measures based on serum IgA or salivary IgA can be avoided. In addition, these indirect measures appear to be poor indicators of duodenal sIgA competence in dogs.
Rinkinen
Minna
M
Department of Clinical Veterinary Sciences, Faculty of Veterinary Medicine, Helsinki University, PO. Box 57, 00014, Helsinki, Finland. Minna.Rinkinen@helsinki.fi
Teppo
Anna-Maija
AM
Harmoinen
Jaana
J
Westermarck
Elias
E
D000818:Animals; D004285:Dogs; D004386:Duodenum / Q000276:immunology*; D005260:Female; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A / Q000097:blood*; D007071:Immunoglobulin A, Secretory / Q000032:analysis*; D008297:Male; D009092:Mucous Membrane / Q000276:immunology*; D012463:Saliva / Q000276:immunology
D007070:Immunoglobulin A; D007071:Immunoglobulin A, Secretory