Cholesterol oxidation using hollow fiber dialyzer immobilized with cholesterol oxidase: preparation and properties.
The surface of polyacrylonitrile hollow fibers were hydrolyzed and covalently bonded with cholesterol oxidase (COD) via glutaraldehyde. The immobilized amount of the COD increased with the concentration of glutaraldehyde. However, COD immobilized with 10% glutaraldehyde had higher activity than with other concentrations. The stabilities of immobilized COD to pH and temperature were higher than those of native enzyme. The immobilized enzyme retained 80% of initial activity after 15 days when stored at 4 degrees C, which was longer than native COD. After being reused six times, the COD-immobilized hollow fiber retained more than 80% of the activity.
Lin
Chi-Chang
CC
Department of Polymer Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan 10672.
Yang
Ming-Chien
MC
D000180:Acrylic Resins / Q000737:chemistry*; D000327:Adsorption; D002784:Cholesterol / Q000737:chemistry*; D002789:Cholesterol Oxidase / Q000737:chemistry*; D003956:Dialysis / Q000295:instrumentation / Q000379:methods*; D015314:Dialysis Solutions / Q000737:chemistry*; D004356:Drug Storage; D004789:Enzyme Activation; D004795:Enzyme Stability; D004800:Enzymes, Immobilized; D005976:Glutaral / Q000737:chemistry*; D008567:Membranes, Artificial; D010084:Oxidation-Reduction; D011522:Protons; D013499:Surface Properties; D013696:Temperature
D000180:Acrylic Resins; D015314:Dialysis Solutions; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D011522:Protons; C010504:polyacrylonitrile; D002784:Cholesterol; D002789:Cholesterol Oxidase; D005976:Glutaral