title: Identification of anther-specific gene expression from T-DNA tagging rice.
abstract: We have screened a total of 5,500 T-DNA tagging rice lines in which beta-glucuronidase (GUS) gene sequence was randomly inserted as a transgene into the plant genome. Histochemical GUS assays were carried out to select the T-DNA tagging rice lines that show its expression in anther. Of the tagging lines screened, three lines were found to express GUS specifically in the anther that is about 0.05%. Microscopic observation of the anther-expressed lines showed specific expression patterns of GUS in the anther, either gametophytic or sporophytic specificities. Southern blot analysis revealed that the integration copy number of the transgene was 2.3 in average. The detailed expression patterns were analyzed and discussed.
authors.affiliation: Graduate School of Life Science and Biotechnology, Korea University, Seoul 136-701, Korea.
authors.forename: Gothandam K
authors.initials: GK
authors.lastname: Muthukalianan
authors.forename: Sanghyun
authors.initials: S
authors.lastname: Lee
authors.forename: Hyunsik
authors.initials: H
authors.lastname: Yum
authors.forename: Sujin
authors.initials: S
authors.lastname: Ku
authors.forename: Minjung
authors.initials: M
authors.lastname: Kwun
authors.forename: Hong Gyu
authors.initials: HG
authors.lastname: Kang
authors.forename: Gynheung
authors.initials: G
authors.lastname: An
authors.forename: Yong-Yoon
authors.initials: YY
authors.lastname: Chung
mesh_terms: D015139:Blotting, Southern; D004269:DNA, Bacterial / Q000032:analysis*; D004274:DNA, Recombinant / Q000032:analysis* / Q000235:genetics; D035264:Flowers / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D005966:Glucuronidase / Q000096:biosynthesis / Q000235:genetics; D009928:Organ Specificity; D012275:Oryza / Q000235:genetics*; D018515:Plant Leaves / Q000378:metabolism; D030821:Plants, Genetically Modified; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D019076:Transgenes
chemical_list: D004269:DNA, Bacterial; D004274:DNA, Recombinant; D011993:Recombinant Fusion Proteins; C036483:T-DNA; D005966:Glucuronidase