title: Structural differences between the alkali-extracted water-soluble cell wall polysaccharides from mycelial and yeast phases of the pathogenic dimorphic fungus Paracoccidioides brasiliensis.
abstract: Paracoccidioides brasiliensis is a pathogenic dimorphic fungus causing paracoccidioidomycosis, the most widespread systemic mycosis in Latin America. We have studied the structure of the alkali-extracted water-soluble cell wall polysaccharides (F1SS) from both mycelial and yeast phases of this fungus by using chemical analysis and NMR spectroscopic techniques. The F1SS polysaccharide from the mycelial phase consists of a trisaccharidic repeating unit of -->6)-[alpha-Galf -(1-->6)-alpha-Manp-(1-->2)]-alpha-Manp-(1-->. The F1SS polysaccharide of the yeast phase maintains 10% of the structure of the mycelium phase, but the main structure contain a disaccharide repeating unit of -->6)-[-alpha-Manp-(1-->2)]-alpha-Manp-(1-->, alternating with a trisaccharide repeating block of -->6)-[beta-Galf -(1-->6)-alpha-Manp-(1-->2)]-alpha-Manp-(1-->.
authors.affiliation: Centro de Investigaciones Biológicas, CSIC, Velázquez 144, 28006-Madrid, Spain.
authors.forename: Oussama
authors.initials: O
authors.lastname: Ahrazem
authors.forename: Alicia
authors.initials: A
authors.lastname: Prieto
authors.forename: Gioconda
authors.initials: G
authors.lastname: San-Blas
authors.forename: Juan Antonio
authors.initials: JA
authors.lastname: Leal
authors.forename: Jesus
authors.initials: J
authors.lastname: Jiménez-Barbero
authors.forename: Manuel
authors.initials: M
authors.lastname: Bernabé
mesh_terms: D002236:Carbohydrate Conformation; D002473:Cell Wall / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D025282:Mycelium / Q000737:chemistry* / Q000235:genetics; D010228:Paracoccidioides / Q000737:chemistry* / Q000254:growth & development / Q000472:pathogenicity; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D015003:Yeasts / Q000737:chemistry* / Q000235:genetics
chemical_list: C438547:F1SS polysaccharide; D011134:Polysaccharides