[Single cell PCR for sex determination in preimplantation genetic diagnosis].
OBJECTIVE
To develop an efficient, rapid and reliable method for sex determination in preimplantation genetic diagnosis.


METHODS
Nested PCR and fluorescent PCR were used to amplify amelogenin and IL-3 genes from human single lymphocytes and blastomeres.


RESULTS
The amplification efficiencies of nested PCR and fluorescent PCR of man single lymphocyte/woman single lymphocyte/blastomere were 64.7%/65%/85.7%, and 72%/76%/86.9% respectively. The PCR products of the lymphocyte were exactly the same as those of the donor gDNA. The PCR products of blastomeres coming from the same embryo were all identical.


CONCLUSIONS
The amplification efficiencies of nested PCR and fluorescent PCR are similar while fluorescent PCR is more conventional, time sparing and sensitive.
Ma
Yan-lin
YL
National Laboratory of Medical Genetics, Central South University, Changsha 410078, China.
Li
Qi
Q
Pan
Qian
Q
D000328:Adult; D001757:Blastomeres / Q000166:cytology; D002469:Cell Separation; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000166:cytology; D008297:Male; D016133:Polymerase Chain Reaction; D019836:Preimplantation Diagnosis; D019849:Sex Determination Processes