Analysis of 3-chlorotyrosine as a specific marker of protein oxidation: the use of N(O,S)-ethoxycarbonyltrifluoroethyl ester derivatives and gas chromatography/mass spectrometry.
N(O,S)-ethoxycarbonyltrifluoroethyl amino acid esters are formed by the reaction of amino acids with ethylchloroformate plus trifluoroethanol plus pyridine. The use of these derivatives for a rapid and sensitive determination of 3-chlorotyrosine, a highly specific marker of myeloperoxidase-catalyzed protein oxidation, by using standard gas chromatography/electron impact mass spectrometry, is discussed.
Pietzsch
Jens
J
Institute of Bioinorganic and Radiopharmaceutical Chemistry, Research Center Rossendorf Dresden, PO Box 51 01 19, 01314 Dresden, Germany. j.pietzsch@fz-rossendorf.de
Kopprasch
Steffi
S
Bergmann
Ralf
R
D002138:Calibration; D004952:Esters / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008077:Lipoproteins, LDL / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry
D004952:Esters; D008077:Lipoproteins, LDL; D011506:Proteins; D014443:Tyrosine; C087259:3-chlorotyrosine