[The role of dendritic cell and macrophage in hepatoma antigen-presenting].
OBJECTIVE
To study the role of dendritic cells (DCs) and macrophages, differentiated from the same individual peripheral blood monocytes, in tumor antigen- presenting.


METHODS
DCs and macrophages were differentiated from human peripheral blood monocytes by adding both Granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) or GM-CSF only. Then they were loaded with tumor antigen at different concentrations and cocultured with autologous T cells in 96-well flat-bottomed microtiter plates for five days at 37 degrees C, 5% CO(2). (3)H-thymine was added before the culture terminated, and twelve hours later, the cells were gathered to test the cpm value.


RESULTS
Both DCs and macrophages chased with tumor antigen could strongly stimulate the proliferation of autologous T cells, especially DCs. The stimulation effect with 20 microl/ml antigen was the most remarkable and the cmp values were 11,950.3 +/-1621.8, 8,708.5 +/-176.1, 402.5+/-43.1 in DCs group, Macrophages group, and lymphocytes group, respectively.


CONCLUSIONS
The antigen presenting role of DCs is stronger than that of macrophages from the same individual.
Li
Yong-guo
YG
Institute of Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China.
Luo
Yun-ping
YP
Liang
Zeng-wei
ZW
Cai
Da-chuan
DC
Lan
Ying-hua
YH
Xie
Qun
Q
Ren
Hong
H
D017951:Antigen Presentation / Q000276:immunology; D000938:Antigen-Presenting Cells / Q000276:immunology / Q000502:physiology*; D000951:Antigens, Neoplasm / Q000276:immunology; D006528:Carcinoma, Hepatocellular / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology / Q000502:physiology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D008113:Liver Neoplasms / Q000276:immunology*; D008264:Macrophages / Q000276:immunology / Q000502:physiology*; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D016178:Granulocyte-Macrophage Colony-Stimulating Factor