Quantification of acetaldehyde released by lung cancer cells in vitro using selected ion flow tube mass spectrometry.
The production of volatile compounds from cancer cell lines in vitro has been investigated using selected ion flow tube mass spectrometry (SIFT-MS). This technique enables on-line quantitative analyses of the headspace above cell/medium cultures. This paper reports the discovery that acetaldehyde is released by the lung cancer cell lines SK-MES and CALU-1. The concentration of acetaldehyde in the headspace of the medium/cell culture was measured after 16 h incubation at 37 degrees C and found to be proportional to the number of cancer cells in the medium (typically 10(8)). From these data, the acetaldehyde production rates of the SK-MES cells and the CALU-1 cells in vitro are determined to be 1 x 10(6) and 1.5-3 x 10(6) molecules/cell/min, respectively. The potential value of this new technique in cell biology and in industrial cell biotechnology is discussed.
Smith
David
D
Centre for Science and Technology in Medicine, School of Postgraduate Medicine, Keele University, Thornburrow Drive, Hartshill, Stoke-on-Trent ST4 7QB, UK. d.smith@bemp.keele.ac.uk
Wang
Tianshu
T
Sulé-Suso
Josep
J
Spanel
Patrik
P
El Haj
Alicia
A
D000079:Acetaldehyde / Q000032:analysis* / Q000378:metabolism; D002452:Cell Count; D007477:Ions / Q000737:chemistry; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D014407:Tumor Cells, Cultured; D014835:Volatilization
D007477:Ions; D000079:Acetaldehyde