Thermococcus atlanticus sp nov., a hyperthermophilic Archaeon isolated from a deep-sea hydrothermal vent in the Mid-Atlantic Ridge [corrected].
An extremely thermophilic archaeon, strain MA898, was isolated from a deep-sea hydrothermal vent on the Mid-Atlantic Ridge. This strain is a strictly anaerobic coccus of approximately 0.7-1.2 microm in diameter. Optimal temperature, pH, and NaCl concentration for growth are around 85 degrees C, pH 7, and 3%, respectively. Strain MA898 grows preferentially in the presence of elemental sulfur, polysulfur, cystine, or L-cysteine. The microorganism requires rich proteinaceous substrates. BHI-S medium supports rapid growth, with a final concentration of more than 1.2 x 10(9) cells ml(-1), but strain MA898 exhibits poor growth on 2216S medium (yeast/peptone) and poor growth on starch. Growth is inhibited by rifampicin and chloramphenicol at a concentration of 100 microg/ml. The DNA G+C content is 50 mol%. Sequencing of the 16S rRNA gene indicates that strain MA898 belongs to the Thermococcusgenus, and from DNA/DNA hybridization data it is proposed as a new species: Thermococcus atlanticus. The deposition numbers are CIP-107420T and DSM15226.
Cambon-Bonavita
Marie-Anne
MA
Laboratoire de Microbiologie et de Biotechnologie des Extrêmophiles, DRV/VP/LMBE, IFREMER Centre de Brest, BP70, 29280 Plouzané, France. macambon@ifremer.fr
Lesongeur
Françoise
F
Pignet
Patricia
P
Wery
Nathalie
N
Lambert
Christophe
C
Godfroy
Anne
A
Querellou
Joël
J
Barbier
Georges
G
D001267:Atlantic Ocean; D001482:Base Composition; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D010802:Phylogeny; D019642:RNA, Archaeal / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology; D013045:Species Specificity; D013696:Temperature; D019712:Thermococcus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D019642:RNA, Archaeal; D012336:RNA, Ribosomal, 16S