[Effect of spinal cord injury on iNOS and Bcl-2 gene expression in spermatogenic cell of the male rat].
OBJECTIVE
To investigate the cause of spermatogenic cells decreasing after spinal cord injury.


METHODS
Immunohistochemical S-P method was used to detect the expression of iNOS and Bcl-2 protein in the testis of spinal cord injured and sham-operated adult rats in second week and fourth week after operation.


RESULTS
Second week and fourth week after operation, the expression of iNOS of operated rats increased significantly (P < 0.05). Fourth week after operation, the number of Bcl-2 positive cells in spinal cord injured group decreased significantly (P < 0.05).


CONCLUSIONS
The expression veition of iNOS and Bcl-2 is the cause that inducing the decrease of spermatogenic cells in testis of spinal cord injured rats.
Wei
Yong
Y
Department of Urology, the Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, China.
Zhang
Xiao-Bin
XB
Zhang
Jie
J
Cheng
Fan
F
D000818:Animals; D008297:Male; D019001:Nitric Oxide Synthase / Q000096:biosynthesis* / Q000235:genetics; D052247:Nitric Oxide Synthase Type II; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000378:metabolism*
D019253:Proto-Oncogene Proteins c-bcl-2; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat