[Cloning of the full length cDNA for a novel leukemia relapse-associated candidate gene LRP15].
To clone the full length cDNA of a novel leukemia relapse-associated candidate gene (LRP15), the human ESTs (Expression Sequence Tags) fragments obtained from electronic hybridization were assembled by a 1.8 kb DNA probe, which was only methylated in relapsed leukemia. Then the primers were designed for rapid amplification of cDNA end (RACE). Bioinformatic data of High Throughout Genomic Sequences (HTGS) and Serial Analysis of Gene Expression (SAGE) were used for chromosome localization and tissue expression analysis. The results showed that the full-length cDNA of the novel gene had an open reading frame of 780 bp encoding a 259 amino acid protein of unkown functions. LRP15 gene expressed in many different tissues was localized in chromosome 3p24. It is concluded that RACE is an effective method to clone novel genes and LRP15 may be a leukemia relapse-associated candidate gene playing an important role in carcinogenesis.
Xu
Zhou-Min
ZM
Department of Hematology, General Hospital of PLA, Beijing 100853, China. xuzhoumin@yahoo.com.cn
Yu
Li
L
Lu
Xue-Chun
XC
Han
Wei-Dong
WD
Li
Xu-Jian
XJ
Jing
Yu
Y
Wang
Shu-Hong
SH
Jin
Hai-Jie
HJ
Lon
Fang-Ding
FD
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D052138:Genes, Neoplasm / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D009364:Neoplasm Recurrence, Local / Q000235:genetics*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000473:pathology; D011506:Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; C477813:LRRC3B protein, human; D009363:Neoplasm Proteins; D011506:Proteins