title: Serum total antioxidant status, erythrocyte superoxide dismutase and whole-blood glutathione peroxidase activities in the Stanislas cohort: influencing factors and reference intervals.
abstract: The aim of this work was to describe the factors influencing the levels of three antioxidant markers -total antioxidant status, erythrocyte copper/zinc superoxide dismutase (SOD), whole-blood selenium glutathione peroxidase--and to establish their reference intervals in supposedly healthy subjects. The studied population included 463 subjects, i.e., 223 adults and 140 children aged 20 to 65 and 4 to 19 years, respectively. The effect of factors such as age, gender, body mass index, alcohol and tobacco consumption, menopause, drug intake, trace elements, transferrin, ferritin, albumin, bilirubin, haptoglobin, total proteins, uric acid, haemoglobin, and mean corpuscular volume of erythrocytes have been studied for the three antioxidant markers. Total antioxidant status (TAS) was higher in men than in women whatever the age (p < 0.001). Albumin and uric acid in men, women and girls, and total proteins in boys were significant determinants of TAS levels. Mean corpuscular volume of erythrocytes were negatively and significantly associated with SOD activity in men and in women (p < 0.01) but not in children. Among the studied determinants, none were found to influence the selenium glutathione peroxidase activity in the four groups. Reference intervals including the 90% confidence intervals were established by age and sex for the three antioxidant markers.
authors.affiliation: INSERM U-525, Centre de Médecine Preventive, Vandoeuvre-Les-Nancy, France.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Habdous
authors.forename: Bernard
authors.initials: B
authors.lastname: Herbeth
authors.forename: Monique
authors.initials: M
authors.lastname: Vincent-Viry
authors.forename: John V
authors.initials: JV
authors.lastname: Lamont
authors.forename: Peters S
authors.initials: PS
authors.lastname: Fitzgerald
authors.forename: Sophie
authors.initials: S
authors.lastname: Visvikis
authors.forename: Gérard
authors.initials: G
authors.lastname: Siest
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000418:Albumins / Q000096:biosynthesis; D000428:Alcohol Drinking; D000975:Antioxidants / Q000378:metabolism*; D001663:Bilirubin / Q000097:blood; D002648:Child; D002675:Child, Preschool; D015331:Cohort Studies; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D005293:Ferritins / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood*; D006242:Haptoglobins / Q000096:biosynthesis; D006454:Hemoglobins / Q000096:biosynthesis; D006801:Humans; D008297:Male; D008593:Menopause; D008875:Middle Aged; D012016:Reference Values; D012044:Regression Analysis; D012737:Sex Factors; D012907:Smoking; D013482:Superoxide Dismutase / Q000097:blood* / Q000378:metabolism; D014131:Trace Elements / Q000097:blood; D014168:Transferrin / Q000096:biosynthesis; D014527:Uric Acid / Q000097:blood
chemical_list: D000418:Albumins; D000975:Antioxidants; D006242:Haptoglobins; D006454:Hemoglobins; D014131:Trace Elements; D014168:Transferrin; D014527:Uric Acid; D005293:Ferritins; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D001663:Bilirubin