title: Gaf-1b is an alternative splice variant of Gaf-1/Rip11.
abstract: Gaf-1/Rip11 encoded by the clone KIAA0857 participates in endosomal recycling through the interaction with both gamma-SNAP, a member of the soluble NSF attachment protein family, and a small GTPase, Rab11. Gaf-1/Rip11 and other Rab11-interacting proteins constitute a novel protein family that is involved in the endocytic pathways. Here we report the presence of an alternative splice variant of Gaf-1/Rip11 named Gaf-1b. Gaf-1b also interacts with gamma-SNAP and is expressed ubiquitously in tissues except for liver. Subcellular fractionation analysis revealed that Gaf-1b, as well as Gaf-1/Rip11, is mainly present in the microsomal fraction. Overexpression of Gaf-1b, like that of Gaf-1/Rip11, affected the morphology of recycling endosomes. These results suggest that Gaf-1b has a similar function to Gaf-1/Rip11.
authors.affiliation: School of Life Science, Tokyo University of Pharmacy and Life Science, Hachioji, Japan.
authors.forename: Kazuho
authors.initials: K
authors.lastname: Kawase
authors.forename: Mika
authors.initials: M
authors.lastname: Shibata
authors.forename: Hoshiko
authors.initials: H
authors.lastname: Kawashima
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Hatsuzawa
authors.forename: Masami
authors.initials: M
authors.lastname: Nagahama
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Tagaya
authors.forename: Katsuko
authors.initials: K
authors.lastname: Tani
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D017398:Alternative Splicing; D002352:Carrier Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D002460:Cell Line; D011992:Endosomes / Q000648:ultrastructure; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D024101:Mitochondrial Proteins; D052067:Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins; D015534:Trans-Activators / Q000032:analysis / Q000235:genetics*; D033921:Vesicular Transport Proteins
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; C485422:RAB11FIP5 protein, human; D052067:Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins; D015534:Trans-Activators; D033921:Vesicular Transport Proteins