Dimethylphthalate hydrolysis by specific microbial esterase.
TWO BACTERIAL STRAINS: Arthrobacter sp. and Sphingomonas paucimobilis were isolated from soil by enrichment cultures using dimethylphthalate (DMP) or monomethylphthalate (MMP) as sole carbon source, respectively. DMP was rapidly transformed by an Arthrobacter sp. culture with formation of MMP and phthalic acid (PA) which is further degraded. This strain was unable to hydrolyse MMP. A mechanism of degradation of DMP was proposed with two ways: DMP-->PA and DMP-->MMP. The S. paucimobilis strain hydrolyses only MMP and a coculture of the two strains allowed a complete degradation of DMP.
Vega
Danielle
D
Centre de Phytopharmacie, UMR CNRS 5054, Université de Perpignan, 66860 Cedex, Perpignan, France.vega@univ-perp.fr
Bastide
Jean
J
D001173:Arthrobacter / Q000201:enzymology; D004950:Esterases / Q000494:pharmacology; D005404:Fixatives / Q000378:metabolism*; D006868:Hydrolysis; D010795:Phthalic Acids / Q000378:metabolism*; D010968:Plasticizers / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D020578:Sphingomonas / Q000201:enzymology
D005404:Fixatives; D010795:Phthalic Acids; D010968:Plasticizers; D012989:Soil Pollutants; C024629:dimethyl phthalate; D004950:Esterases