New PCR test that recognizes all human prototypes of enterovirus: application for clinical diagnosis.
We describe a new PCR test (Penter RT-PCR) that recognizes all 64 prototypes of enterovirus. Sixty clinical samples were analyzed in parallel with this Penter RT-PCR and previously described PCR tests: 34 and 32 samples tested positive, respectively. This assay is suitable for use in clinical diagnosis, and its ability to amplify all known serotypes makes it more useful than other consensus PCR tests.
Bourlet
Thomas
T
Laboratoire de Bactériologie-Virologie, GIMAP, Faculté de Médecine, St-Etienne, France.
Caro
Valerie
V
Minjolle
Sophie
S
Jusselin
Isabelle
I
Pozzetto
Bruno
B
Crainic
Radu
R
Colimon
Ronald
R
D004770:Enterovirus / Q000145:classification* / Q000235:genetics*; D004769:Enterovirus Infections / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012703:Serotyping