Effects of selenium and iodine on c-fos and c-jun mRNA and their protein expressions in cultured rat hippocampus cells.
The objective of the study was to investigate the effect of selenium (Se) and iodine (I) for expressions of c-fos and c-jun mRNA and their proteins in cultured rat hippocampus cells in selenium- and iodine-containing medium. With cultured rat hippocampus cells, the different doses of Se and I were added into the medium. The expressions of c-fos/c-jun in cultured rat hippocampus cells (1 d, 3 d, 5 d, 7 d, and 10 d) were studied by using both in situ hybridization histochemistry and SABC immunohistochemistry techniques. Both Se and I could enhance expressions of c-fos and c-jun mRNA and their proteins, especially c-jun mRNA expression in the Se and I united group. Se and I could promote the expressions of c-fos and c-jun and thus may affect the differentiation and development of hippocampus neurons.
Tian
Dongping
D
Department of Pathology, Shantou University Medical College, Guangdong, 515031 China.
Su
Min
M
Xu
Xiaohu
X
Wu
Xianying
X
Li
Qiaoshan
Q
Zheng
Rueiming
R
D000818:Animals; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects*; D016762:Genes, fos / Q000235:genetics; D016758:Genes, jun / Q000235:genetics; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D007150:Immunohistochemistry; D007455:Iodine / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis* / Q000235:genetics*; D016755:Proto-Oncogene Proteins c-jun / Q000096:biosynthesis* / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D012643:Selenium / Q000494:pharmacology*
D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D007455:Iodine; D012643:Selenium