Practical issues in the application of oxygenases.
Oxygenases carry out the regio-, stereo- and chemoselective introduction of oxygen in a tremendous range of organic molecules. This versatility has already been exploited in several commercial processes. There are, however, many hurdles to further practical large-scale applications. Here, we review various issues in biocatalysis using these enzymes, such as screening strategies, overoxidation, uncoupling, substrate uptake, substrate toxicity, and oxygen mass transfer. By addressing these issues in a systematic way, the productivity of promising laboratory scale biotransformations involving oxygenases may be improved to levels that allow industry to realise the full commercial potential of these enzymes.
van Beilen
Jan B
JB
Institute of Biotechnology, ETH Hönggerberg, CH-8093 Zürich, Switzerland. vanbeilen@biotech.biol.ethz.ch
Duetz
Wouter A
WA
Schmid
Andreas
A
Witholt
Bernard
B
D019149:Bioreactors; D002617:Chemical Industry / Q000379:methods*; D004345:Drug Industry / Q000379:methods*; D004789:Enzyme Activation; D004795:Enzyme Stability; D010105:Oxygenases / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods*; D013379:Substrate Specificity
D010105:Oxygenases