CD27 is heterogeneously expressed in multiple myeloma: low CD27 expression in patients with high-risk disease.
Expression of CD27 on malignant plasma cells (PC) (CD138+CD38++) was analysed in a cross-sectional study of bone marrow (BM) samples from multiple myeloma (MM) patients (n = 28), monoclonal gammopathy of undetermined significance (MGUS) patients (n = 6) and BM PC from healthy donors (n = 4). MM PC expressed CD27 with a variable, lower intensity pattern compared with the consistent high expression in MGUS and healthy donors. MM patients in complete clinical remission displayed a higher percentage of CD27+ PC compared with patients at diagnosis, relapse or in partial remission. In MM, loss of CD27 correlated with loss of CD19 (R2 = 0.4, P < 0.0001). Human MM cell lines (n = 9) did not express CD27 whereas de novo plasma cell leukaemia (PCL) (n = 3) had a high expression. Re-analysis of a cDNA microarray data set, generated from newly diagnosed MM patients (n = 74), demonstrated that the MM subgroup with the highest prevalence of poor prognostic factors had the lowest CD27 mRNA expression. Fluorescence-activated cell sorting and allele-specific oligonucleotide polymerase chain reaction showed that both CD27+ and CD27- PC subpopulations in MM can belong to the clonal disorder. In conclusion, CD27 is heterogeneously expressed on MM PC and loss of CD27 expression might have prognostic value in MM.
Guikema
Jeroen E J
JE
Department of Cell Biology, Histology and Immunology Section, University Hospital, University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands.
Hovenga
Sjoerd
S
Vellenga
Edo
E
Conradie
Jelle J
JJ
Abdulahad
Wayel H
WH
Bekkema
Roelof
R
Smit
Jan W
JW
Zhan
Fenghuang
F
Shaughnessy
John
J
Bos
Nicolaas A
NA
D036541:ADP-ribosyl Cyclase / Q000032:analysis; D051997:ADP-ribosyl Cyclase 1; D015703:Antigens, CD / Q000032:analysis; D016022:Case-Control Studies; D006801:Humans; D016130:Immunophenotyping; D008562:Membrane Glycoproteins; D009101:Multiple Myeloma / Q000276:immunology*; D020411:Oligonucleotide Array Sequence Analysis; D010265:Paraproteinemias / Q000276:immunology; D010950:Plasma Cells / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000032:analysis*
D015703:Antigens, CD; D008562:Membrane Glycoproteins; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D036541:ADP-ribosyl Cyclase; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1