Compared protective effect of nasal immunoprophylaxis using a new human monoclonal IgM antibody, human polyclonal antibodies, F(ab')2, amantadine, and zanamivir for prophylaxis of influenza A virus pneumonia in mice.
This study evaluated the effectiveness of five human monoclonal antibodies in comparison with human polyclonal antibodies, F(ab')2, amantadine, and zanamivir in a mouse model of lethal influenza A virus infection. A single intranasal administration of antibodies was done for immunoprophylaxis. Zanamivir was administered intranasally and amantadine was administered orally. Mice were fully protected by a single dose of 30 mg/kg intravenous Ig administered at least 3 days before challenge. This treatment was equivalent to zanamivir. F(ab')2 were less effective (p < 0.001). One of the monoclonal antibodies tested was less efficient than both zanamivir (p < 0.001) and intravenous Ig (p < 0.001) but similar to amantadine.
Hernandez
Eric
E
Centre d'Etudes du Bouchet, Laboratoire de Microbiologie, BP3, 91710 Vert-le-Petit, France.
Ramisse
Françoise
F
Lhonneux
Anne
A
Noury
Jacques
J
Bazin
Hervé
H
Cavallo
Jean-Didier
JD
D000547:Amantadine / Q000627:therapeutic use*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D000998:Antiviral Agents / Q000627:therapeutic use*; D005260:Female; D006146:Guanidines; D006801:Humans; D007116:Immunization, Passive; D007140:Immunoglobulin Fab Fragments / Q000627:therapeutic use*; D007075:Immunoglobulin M / Q000627:therapeutic use*; D016756:Immunoglobulins, Intravenous / Q000627:therapeutic use*; D009980:Influenza A virus; D007251:Influenza, Human / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D011024:Pneumonia, Viral / Q000517:prevention & control*; D011714:Pyrans; D012794:Sialic Acids / Q000627:therapeutic use*; D053243:Zanamivir
D000911:Antibodies, Monoclonal; D000998:Antiviral Agents; D006146:Guanidines; D007140:Immunoglobulin Fab Fragments; D007075:Immunoglobulin M; D016756:Immunoglobulins, Intravenous; D011714:Pyrans; D012794:Sialic Acids; D000547:Amantadine; D053243:Zanamivir