Development and validation of a method for the confirmation of halofuginone in chicken liver and eggs using electrospray tandem mass spectrometry.
A method is described for the quantitative confirmation of halofuginone (HFG) residues in chicken liver and eggs. This method is based on LC coupled to positive ion electrospray MS-MS of the tissue extracts, prepared by trypsin digestion of the tissues followed by liquid-liquid extraction and final clean-up using Solid Phase Extraction (SPE). The [M+H](+) ion at m/z 416 is monitored along with four transitions at m/z 398, 138, 120 and 100. The method has been validated according to the draft EU criteria for the analysis of veterinary drug residues at 15, 30 and 45 microg kg(-1) in liver and 5, 15 and 50 microg kg(-1) in eggs. The new analytical limits, CCalpha and CCbeta were calculated for liver and were 35.4 and 43.6 microg kg(-1), respectively.
Yakkundi
S
S
Department of Veterinary Science, Queen's University Belfast, Northern Ireland, UK.
Cannavan
A
A
Elliott
C T
CT
Lövgren
T
T
Kennedy
D G
DG
D002138:Calibration; D003049:Coccidiostats / Q000032:analysis*; D004531:Eggs / Q000032:analysis*; D008099:Liver / Q000737:chemistry*; D010880:Piperidines; D011799:Quinazolines / Q000032:analysis*; D052999:Quinazolinones; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D003049:Coccidiostats; D010880:Piperidines; D011799:Quinazolines; D052999:Quinazolinones; C010176:halofuginone