title: Mammalian twisted gastrulation is essential for skeleto-lymphogenesis.
abstract: Dorsoventral patterning depends on the local concentrations of the morphogens. Twisted gastrulation (TSG) regulates the extracellular availability of a mesoderm inducer, bone morphogenetic protein 4 (BMP-4). However, TSG function in vivo is still unclear. We isolated a TSG cDNA as a secreted molecule from the mouse aorta-gonad-mesonephros region. Here we show that TSG-deficient mice were born healthy, but more than half of the neonatal pups showed severe growth retardation shortly after birth and displayed dwarfism with delayed endochondral ossification and lymphopenia, followed by death within a month. TSG-deficient thymus was atrophic, and phosphorylation of SMAD1 was augmented in the thymocytes, suggesting enhanced BMP-4 signaling in the thymus. Since BMP-4 promotes skeletogenesis and inhibits thymus development, our findings suggest that TSG acts as both a BMP-4 agonist in skeletogenesis and a BMP-4 antagonist in T-cell development. Although lymphopenia in TSG-deficient mice would partly be ascribed to systemic effects of runtiness and wasting, our findings may also provide a clue for understanding the pathogenesis of human dwarfism with combined immunodeficiency.
authors.affiliation: Division of Hematopoietic Factors, Advanced Clinical Research Center, Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan. tenosaka@ims.u-tokyo.ac.jp
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Nosaka
authors.forename: Sumiyo
authors.initials: S
authors.lastname: Morita
authors.forename: Hidetomo
authors.initials: H
authors.lastname: Kitamura
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nakajima
authors.forename: Fumi
authors.initials: F
authors.lastname: Shibata
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Morikawa
authors.forename: Yuki
authors.initials: Y
authors.lastname: Kataoka
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Ebihara
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Kawashima
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Itoh
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Ozaki
authors.forename: Emiko
authors.initials: E
authors.lastname: Senba
authors.forename: Kohichiro
authors.initials: K
authors.lastname: Tsuji
authors.forename: Fusao
authors.initials: F
authors.lastname: Makishima
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Yoshida
authors.forename: Toshio
authors.initials: T
authors.lastname: Kitamura
mesh_terms: D000818:Animals; D001846:Bone Development / Q000235:genetics* / Q000502:physiology; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins / Q000819:agonists* / Q000037:antagonists & inhibitors*; D002454:Cell Differentiation; D050676:Core Binding Factor Alpha 2 Subunit; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005314:Embryonic and Fetal Development / Q000235:genetics; D018390:Gene Targeting; D006130:Growth Disorders / Q000235:genetics / Q000473:pathology; D006801:Humans; D017403:In Situ Hybridization; D007668:Kidney / Q000002:abnormalities; D008221:Lymphoid Tissue / Q000196:embryology* / Q000254:growth & development; D008231:Lymphopenia / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout; D010641:Phenotype; D011506:Proteins / Q000235:genetics* / Q000502:physiology; D011518:Proto-Oncogene Proteins; D015398:Signal Transduction; D051785:Smad Proteins; D051898:Smad1 Protein; D013601:T-Lymphocytes / Q000166:cytology; D015534:Trans-Activators / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics
chemical_list: C525809:BMP4 protein, human; C525810:Bmp4 protein, mouse; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins; D050676:Core Binding Factor Alpha 2 Subunit; D004268:DNA-Binding Proteins; D011506:Proteins; D011518:Proto-Oncogene Proteins; C497399:SMAD1 protein, human; D051785:Smad Proteins; D051898:Smad1 Protein; C497401:Smad1 protein, mouse; D015534:Trans-Activators; D014157:Transcription Factors; C473561:twisted gastrulation protein, mouse