Hematopoietic progenitor colony formation in the immunopathogenesis of the autoimmune disorder in MRL/MpJ-lpr mice.
Lymphocyte proliferation and apoptosis at different stages of the development of the autoimmune disorder in MRL/MpJ-lpr mice was studied. Hematopoietic progenitor colony formation during the course of the disease was characterized. A detectable difference at the level of lymphocyte proliferation, apoptosis, and the relative amount of BFU-E, CFU-GM and CFU-GEMM cell colonies was revealed between healthy young mice and animals spontaneously developing pronounced symptoms of the autoimmune disorder. The quantity of BFU-E and CFU-GEMM colonies was remarkably increases in aged MRL/MpJ-lpr mice even before clinical manifestation of the disease (proteinuria). An elevated number of CFU-GEMM was accompanied by a striking increase in their size. The study of hematopoietic disturbances in autoimmune MRL/MpJ-lpr mice may be very useful for understanding the mechanism of the autoimmune disease development and searching for new strategies of the correction of the autoimmune disorder.
Toporkova
Ludmila B
LB
Institute of Clinical Immunology, Siberian Branch of RAMS, Yadrinzevskaya str. 14, Novosibirsk 630099, Russia.
Dubrovskaya
Viktoriya V
VV
Sakhno
Ludmila V
LV
Tikhonova
Marina A
MA
Chernykh
Elena R
ER
Buneva
Valentina N
VN
Nevinsky
Georgy A
GA
Kozlov
Vladimir A
VA
Orlovskaya
Irina A
IA
D000818:Animals; D017209:Apoptosis / Q000276:immunology; D001327:Autoimmune Diseases / Q000209:etiology* / Q000276:immunology* / Q000473:pathology; D002455:Cell Division / Q000276:immunology; D002460:Cell Line; D006412:Hematopoietic Stem Cells / Q000276:immunology* / Q000473:pathology; D066298:In Vitro Techniques; D008198:Lymph Nodes / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D019463:Mice, Inbred MRL lpr; D011507:Proteinuria / Q000235:genetics / Q000378:metabolism