[Catalysis of lyase-isomerase PecE/PecF for several apophycobiliproteins].
Phycoerythrocyanin(PEC) lyase-isomerase PecE/PecF from Mastigocladus laminosus is the specific enzyme for biosynthesis of PEC alpha-subunit(alpha-PEC). In this work, the specificity of PecE/PecF on substrate apoproteins was reported. PecE/PecF could catalyse the reconstitution of phycocyanobilin(PCB) with apoproteins of alpha-PEC from two different subspecies of Mastigocladus laminosus, as well the site-directed mutated apoprotein of alpha-PEC with Trp at 128 to Phe in vitro, but could not catalyse the reconstitution of PCB with apoprotein of phycocyanin alpha-subunit(alpha-CPC) from Mastigocladus laminosus. The surfactant Triton X-100 had no effect for the reconstitution of alpha-PEC, while it could improve the reconstitution of PCB with apoprotein of alpha-CPC.
Zhu
Jing-Ping
JP
School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Zhou
Ming
M
Zhao
Kai-Hong
KH
Zeng
Zhi-Xiong
ZX
Zhou
Yi-Kai
YK
D001059:Apoproteins / Q000378:metabolism*; D001426:Bacterial Proteins; D002384:Catalysis; D000458:Cyanobacteria / Q000201:enzymology; D045342:Light-Harvesting Protein Complexes; D008190:Lyases / Q000378:metabolism*; D017830:Octoxynol / Q000494:pharmacology; D011506:Proteins / Q000378:metabolism*; D013379:Substrate Specificity
D001059:Apoproteins; D001426:Bacterial Proteins; D045342:Light-Harvesting Protein Complexes; D011506:Proteins; D017830:Octoxynol; D008190:Lyases; C093743:phycoerythrocyanin alpha-subunit phycobiliviolin lyase protein, bacteria