title: Evidence for hair cell regeneration in the crista ampullaris of the lizard Podarcis sicula.
abstract: We studied hair cell regeneration in the crista ampullaris of the lizard Podarcis sicula both in untreated animals and at early and late time intervals following a single high dose of gentamicin. The study was carried out using the S-phase marker 5-bromo-2'-deoxyuridine. Our ultrastructural and immunofluorescence studies showed that both apoptosis and hair cell regeneration happen in the lizard crista ampullaris in untreated animals, and that regenerative processes are greatly accelerated after treatment with the aminoglycoside antibiotic gentamicin. Our observations indicate that hair cell regeneration is strongly implicated in the repair of damaged sensory epithelium, and that new hair cells appear likely to arise from supporting cells.
authors.affiliation: Department of Genetics, General and Molecular Biology, University Federico II, via Mezzocannone 8, 80134, Naples, Italy.
authors.forename: Bice
authors.initials: B
authors.lastname: Avallone
authors.forename: Maria
authors.initials: M
authors.lastname: Porritiello
authors.forename: Daniela
authors.initials: D
authors.lastname: Esposito
authors.forename: Rosalia
authors.initials: R
authors.lastname: Mutone
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Balsamo
authors.forename: Francesco
authors.initials: F
authors.lastname: Marmo
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D017209:Apoptosis; D001973:Bromodeoxyuridine; D005455:Fluorescent Antibody Technique; D005839:Gentamicins / Q000494:pharmacology; D006198:Hair Cells, Auditory / Q000187:drug effects / Q000502:physiology*; D008116:Lizards / Q000033:anatomy & histology / Q000502:physiology*; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D012038:Regeneration / Q000187:drug effects; D012665:Semicircular Canals / Q000166:cytology / Q000502:physiology*; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins; D001973:Bromodeoxyuridine