title: Evaluation of the BDProbeTec ET system for direct detection of Mycobacterium tuberculosis in pulmonary and extrapulmonary samples: a multicenter study.
abstract: We evaluated the BDProbeTec ET system (Becton Dickinson, Sparks, Md.), a strand displacement amplification-based technique, for direct detection of Mycobacterium tuberculosis in 867 clinical samples. Of 294 extrapulmonary specimens, 52 had positive results by both BDProbeTec ET and culture and 209 had negative results by both methods; sensitivity and specificity were 76.5 and 95.9%, respectively. After resolution of discrepancies, the sensitivity rose to 77.8%.
authors.affiliation: Microbiology and Virology Laboratory, Regional Reference Center for Mycobacteria, Careggi Hospital, Florence, Italy.
authors.forename: Gianna
authors.initials: G
authors.lastname: Mazzarelli
authors.forename: Laura
authors.initials: L
authors.lastname: Rindi
authors.forename: Paola
authors.initials: P
authors.lastname: Piccoli
authors.forename: Claudio
authors.initials: C
authors.lastname: Scarparo
authors.forename: Carlo
authors.initials: C
authors.lastname: Garzelli
authors.forename: Enrico
authors.initials: E
authors.lastname: Tortoli
mesh_terms: D001826:Body Fluids / Q000382:microbiology; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012137:Respiratory System / Q000382:microbiology; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000382:microbiology*; D014397:Tuberculosis, Pulmonary / Q000382:microbiology*
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic