[The correlated study on CREM expression and testicular breeding sperm functional lesion in rats with varicocele].
OBJECTIVE
To study male infertility's mechanism caused by varicocele.


METHODS
30 rats were selected at random, of 20 were operated, as varicocele's group;of 10 were pseudo-operated, as controlled group, testes were removed, stained and sectioned. Makler Score were adopted to analyse the bore, limitan's thickness, layer number of cell, the degree of cell's maturity and average score in 200 and 100 seminiferous tubulae respectively. CREM was determined by hybridization in situ, the difference between two groups was compared.


RESULTS
In varicocele' group, seminiferous tubule's bore was decreased (101.2 +/- 2.2 vs 146.0 +/- 4.1), limitan became thicker (3.5 +/- 0.1 vs 1.9 +/- 0.2), cell' s layer number was reduced (3.0 +/- 0.2 vs 5.5 +/- 0.1), cell's maturity turned to disturbance (3.6 +/- 0.3 vs 4.9 +/- 0.1), the average score was lower than controlled group (16.0 +/- 1.2 vs 8.5 +/- 0.6), they had significant differences (P < 0.001). CREM expression was lower in varicocele group than in controlled one (VG 2.0 +/- 0.32, SoG 3.90 +/- 0.32) (P < 0.001), which was located in spermatogenous cell to spermatocyte stage. The quantu of expression and score value brought out positive correlation (r = 0.746, P < 0.001).


CONCLUSIONS
CREM lower expression could lead to breeding sperm functional lesion, which probably was one of the mechanisms of infertility caused by varicocele.
Yu
Jianjun
J
Urological department, 1st affiliated hospital, Zhejiang University, Hangzhou 310003, China.
Chen
Zhaodian
Z
D000818:Animals; D051786:Cyclic AMP Response Element Modulator; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D007248:Infertility, Male / Q000209:etiology / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012097:Repressor Proteins; D013091:Spermatogenesis; D013094:Spermatozoa; D013733:Testicular Diseases / Q000378:metabolism* / Q000503:physiopathology; D014646:Varicocele / Q000378:metabolism* / Q000503:physiopathology
D004268:DNA-Binding Proteins; D012097:Repressor Proteins; D051786:Cyclic AMP Response Element Modulator