title: Capillary zone electrophoresis in wide bore capillary tubes with fiber-coupled diode array detection.
abstract: This feasibility study deals with the use of a wide bore (320 microm I.D.) capillary tube for the detection and identification of capillary zone electrophoresis (CZE) analytes by optical fiber-coupled diode array detection. A 250-microm mean effective pathlength of the detection cell with an inherently enhanced photon flux through the cell were significant contributors in reaching 0.2-1 micromol/l concentration detectabilities of the CZE analytes by this combination. Experiments with model analytes (p-sulfanilic, sorbic and naphthalene-2-sulfonic acids, tryptophan and asulam) revealed that spectral confirmations of their identities were still possible when their concentrations in the loaded samples (200 nl) were 1-5 micromol/l. Here, chemometry procedures (target transformation factor analysis, fixed size moving window-target transformation factor analysis, fixed size moving window-evolving factor analysis and orthogonal projection approach) employed in the data processing effectively contributed to reliable confirmation of the identities of the analytes also in critical situations (e.g. peak overlaps). The CZE separations were carried out in tandem-coupled columns of identical I.D. This made it possible to use, in the first column of the tandem, carrier electrolyte solutions that provide the desired separative effects, while in the second (detection) column the compositions of the carrier electrolyte solutions employed could reflect favorable conditions for obtaining spectral data. Mixtures containing model constituents at significantly differing concentrations and Maillard's reaction products spiked with tryptophan enantiomers were employed in experiments aimed at assessing practical applicabilities and limits of the present approach to the analysis of samples characterized by complex ionic matrices.
authors.affiliation: Department of Analytical Chemistry, Faculty of Natural Sciences, Comenius University, Mlynská Dolina CH-2, SK-84215 Bratislava, Slovak Republic.
authors.forename: Stanislav
authors.initials: S
authors.lastname: Strasík
authors.forename: Mariana
authors.initials: M
authors.lastname: Danková
authors.forename: Markéta
authors.initials: M
authors.lastname: Molnárová
authors.forename: Eva
authors.initials: E
authors.lastname: Olvecká
authors.forename: Dusan
authors.initials: D
authors.lastname: Kaniansky
mesh_terms: D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet / Q000295:instrumentation / Q000379:methods*; D013237:Stereoisomerism