title: Application of purging biotinylated liposomes from plasma to elucidate influx and efflux processes associated with accumulation of liposomes in solid tumors.
abstract: Although small, 100-nm liposomes are known to selectively accumulate in solid tumors, the individual contributions of liposome influx and egress rates are not well understood. The aim of this work was to determine influx and efflux kinetics for 100-nm, 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC)/cholesterol (Chol) liposomes by inducing aggregate formation of biotinylated liposomes upon administering avidin. Injecting 50 microg of neutravidin intravenously to mice that had previously been administered 100 mg/kg DPSC/Chol liposomes containing 0.5 mol% biotin-conjugated lipid resulted in >90% elimination of the liposomes from plasma within 1 h. This rapid removal by the reticuloendothelial system (RES) permitted the determination of the tumor efflux kinetics due to negligible tumor influx after neutravidin injection. The tumor efflux rate constant (k(-1)) was determined to be 0.041 h(-1) when neutravidin was injected 4 h after liposome injection. This allowed the determination of the tumor influx rate constant (k(1)), which under these conditions was 0.022 h(-1). Therefore, DSPC/Chol liposomal accumulation, in LS180 solid tumors, is dictated primarily by plasma liposome concentrations and liposome egress is comparable or slightly faster than influx into the tumors. This method is applicable for a wide range of lipid doses, and can be used to characterize influx and efflux parameters at different time points after accumulation. The application, therefore, has the potential to be used to fully characterize the impact of different liposome parameters such as lipid composition, steric stabilization, size and dose on tumor accumulation kinetics.
authors.affiliation: Division of Pharmaceutics, Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Vincent W H
authors.initials: VW
authors.lastname: Fung
authors.forename: Gigi N C
authors.initials: GN
authors.lastname: Chiu
authors.forename: Lawrence D
authors.initials: LD
authors.lastname: Mayer
mesh_terms: D000818:Animals; D001360:Avidin / Q000494:pharmacology; D001710:Biotin; D003110:Colonic Neoplasms / Q000097:blood / Q000098:blood supply / Q000737:chemistry / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D008081:Liposomes / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D051379:Mice; D010713:Phosphatidylcholines / Q000737:chemistry; D013997:Time Factors; D014018:Tissue Distribution
chemical_list: D008081:Liposomes; D010713:Phosphatidylcholines; C093483:neutravidin; D001360:Avidin; D001710:Biotin; C010942:1,2-distearoyllecithin