title: Chromatin immunoprecipitation reveals that the 180-bp satellite repeat is the key functional DNA element of Arabidopsis thaliana centromeres.
abstract: The centromeres of Arabidopsis thaliana chromosomes contain megabases of complex DNA consisting of numerous types of repetitive DNA elements. We developed a chromatin immunoprecipitation (ChIP) technique using an antibody against the centromeric H3 histone, HTR12, in Arabidopsis. ChIP assays showed that the 180-bp centromeric satellite repeat was precipitated with the antibody, suggesting that this repeat is the key component of the centromere/kinetochore complex in Arabidopsis.
authors.affiliation: Department of Horticulture, University of Wisconsin, Madison, Wisconsin 53706, USA.
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Nagaki
authors.forename: Paul B
authors.initials: PB
authors.lastname: Talbert
authors.forename: Cathy Xiaoyan
authors.initials: CX
authors.lastname: Zhong
authors.forename: R Kelly
authors.initials: RK
authors.lastname: Dawe
authors.forename: Steven
authors.initials: S
authors.lastname: Henikoff
authors.forename: Jiming
authors.initials: J
authors.lastname: Jiang
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics; D002503:Centromere / Q000235:genetics*; D002843:Chromatin / Q000302:isolation & purification*; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics*
chemical_list: D029681:Arabidopsis Proteins; D002843:Chromatin; D018744:DNA, Plant