title: [Cloning of human monoclonal Fab fragments against HIV-1 gp120 peptide binding chemokine receptor from phage Fab antibody library].
abstract: OBJECTIVE
  To screen human monoclonal Fab fragments against HIV-1 gp120 peptide binding chemokine receptor.
  
  
  METHODS
  A synthesized polypeptide containing 23 amino acid residues of the gp120 antigen epitope binding chemokine receptor was coated as the solid-phase antigen. After biopanning from the HIV-1 phage Fab antibody library, the acquired positive clones were tested and sequenced.
  
  
  RESULTS
  One clone of human phage Fab monoclonal antibody against HIV-1 gp120 polypeptide was acquired. It has high affinity, specificity and inhibition rate and it belongs to IgG I subclass and kappa type. Its Vh H and V kappa were derived from Vh III and V kappa III.
  
  
  CONCLUSIONS
  The human phage Fab fragment against HIV-1 gp120 antigen site binding chemokine receptor was acquired.
authors.affiliation: Department of Virology, The 302nd Hospital of PLA, Beijing 100039, China.
authors.forename: Honghui
authors.initials: H
authors.lastname: Shen
authors.forename: Panyong
authors.initials: P
authors.lastname: Mao
authors.forename: Shiwen
authors.initials: S
authors.lastname: Hong
authors.forename: Jun
authors.initials: J
authors.lastname: Hou
authors.forename: Jianyang
authors.initials: J
authors.lastname: Yang
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000235:genetics*; D000914:Antibodies, Viral / Q000235:genetics*; D001435:Bacteriophages / Q000235:genetics; D003001:Cloning, Molecular; D015699:HIV Envelope Protein gp120; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000235:genetics*; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D019151:Peptide Library; D019707:Receptors, Chemokine / Q000378:metabolism; D015728:Receptors, HIV / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D015699:HIV Envelope Protein gp120; D007140:Immunoglobulin Fab Fragments; D019151:Peptide Library; D019707:Receptors, Chemokine; D015728:Receptors, HIV