title: Bacterial kidney disease as a model for studies of cell mediated immunity in rainbow trout (Oncorhynchus mykiss).
abstract: A cell mediated immune (CMI) response was measured in vitro to heat-killed and to paraformaldehyde fixed Renibacterium salmoninarum (Rs) in rainbow trout (Oncorhynchus mykiss) experimentally challenged with live Rs. The mitogenic response to the T lymphocyte mitogen Concanavalin A (Con A) was reduced during samplings 4 to 6 weeks after immersion, but no effect of the response to the B lymphocyte mitogen lipopolysaccharide (LPS) was detected. The subpopulation of lymphocytes, detected by the monoclonal antibody 1C2, was decreased from the 4th week to the 5th week of infection, and remained at the decreased level up to 10 weeks post immersion. The proportion of Immunoglobulin (Ig) bearing lymphocytes was not affected during the Rs infection period. The humoral antibody level to heat-stable Rs-antigens was increased up to 10 weeks after immersion but after 27 weeks was reduced to a level similar to that of the non-challenged fish. An anamnestic response was demonstrated in challenged fish, as intraperitoneal injection of heat-treated Rs bacteria into Rs challenged fish elicited a stronger humoral antibody response compared with injection into non-challenged fish.
authors.affiliation: National Veterinary Institute, Department of Fish, SE-75189 Uppsala, Sweden. eva.jansson@sva.se
authors.forename: Eva
authors.initials: E
authors.lastname: Jansson
authors.forename: Thorbjörn
authors.initials: T
authors.lastname: Hongslo
authors.forename: Anders
authors.initials: A
authors.lastname: Johannisson
authors.forename: Lars
authors.initials: L
authors.lastname: Pilström
authors.forename: Sirje
authors.initials: S
authors.lastname: Timmusk
authors.forename: Leif
authors.initials: L
authors.lastname: Norrgren
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D015152:Blotting, Northern / Q000662:veterinary; D003208:Concanavalin A / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005393:Fish Diseases / Q000276:immunology / Q000382:microbiology*; D005434:Flow Cytometry / Q000662:veterinary; D016908:Gram-Positive Bacterial Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D007674:Kidney Diseases / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000276:immunology; D016131:Lymphocyte Subsets / Q000276:immunology / Q000382:microbiology; D008835:Micrococcaceae; D017686:Oncorhynchus mykiss / Q000276:immunology*; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics
chemical_list: D000907:Antibodies, Bacterial; D008070:Lipopolysaccharides; D012329:RNA, Bacterial; D003208:Concanavalin A