title: [Separation of adhering cell colonies with a direct digestion method].
abstract: OBJECTIVE
  To establish a highly efficient method for the separation of adhering cell colonies.
  
  
  METHODS
  Cell suspension was diluted gradiently to four different densities and then seeded on a cell culture plate covered with parted fibrinous membranes. After colonies appeared, they were digested directly with 0.25% trypsin and picked out with a pipette tip.
  
  
  RESULTS
  At the density of 5-10.cm-2, colonies could be separated most efficiently in both continuous and normal diploid cell line colonies. The cell number could reach 10(6) after one month of culture for a continuous cell line colony and one and a half month of culture for a normal diploid cell line colony.
  
  
  CONCLUSIONS
  With the direct digestion method, single cell colonies can be effectively separated from the plate covered with parted fibrinous membranes, when the cell was initially seeded at the density of 5-10.cm2.
authors.affiliation: National Laboratory of Medical Genetics, Central South University, Changsha 410078, China.
authors.forename: Di-cheng
authors.initials: DC
authors.lastname: Zhao
authors.forename: Zhi-gao
authors.initials: ZG
authors.lastname: Long
authors.forename: Duo
authors.initials: D
authors.lastname: Zheng
mesh_terms: D000818:Animals; D016466:CHO Cells / Q000166:cytology*; D002469:Cell Separation; D002478:Cells, Cultured; D006224:Cricetinae; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D013234:Stem Cells / Q000166:cytology*; D014471:Umbilical Veins / Q000166:cytology