Variant alleles on the penta E locus in the PowerPlex 16 kit.
Penta E in the PowerPlex 16 kit is a pentanucleotide tandem repeat marker located on Chromosome 15, containing an AAAGA repeat motif. Variant alleles (18.4 and 19.4) were found in the Japanese population. A sequence analysis revealed that both the variant alleles had a partial repeat motif of AAAA, resulting in one-base-shorter alleles compared to known alleles. Despite the relatively large amplicon sizes (379 to 474 bp) of Penta E, an accurate allele assignment can be reliably made by capillary electrophoresis. However, alleles differing in size by only one base (e.g., 18.4 and 19) were not separated and appeared as a single broad peak. The Genotyper software assigned one of the component alleles to this peak. Therefore, such broad peaks require careful interpretation so as to not overlook the other component allele contained by the peak. As an index to recognize a peak containing two alleles, the ratio of peak area to peak height was found to be useful.
Mizuno
Natsuko
N
Biology Section, Department of First Forensic Science, National Research Institute of Police Science, 6-3-1 Kashiwanoha Kashiwa, Chiba, 277-0082, Japan. mizuno@nrips.go.jp
Sekiguchi
Kazumasa
K
Sato
Hajime
H
Kasai
Kentaro
K
D000483:Alleles; D002884:Chromosomes, Human, Pair 15; D016172:DNA Fingerprinting / Q000379:methods; D019075:Electrophoresis, Capillary; D005554:Forensic Medicine / Q000379:methods; D005819:Genetic Markers; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D044469:Racial Groups / Q000235:genetics; D017422:Sequence Analysis, DNA; D012984:Software; D020080:Tandem Repeat Sequences
D005819:Genetic Markers