Sequence analysis demonstrates the conservation of fimH and variability of fimA throughout avian pathogenic Escherichia coli (APEC).
In this study we sequenced and analysed the fimH and fimA genes of 24 avian pathogenic Escherichia coli (APEC) isolates, in order to investigate their possible conserved nature. Additional parameters (serotype, presence of aerobactin receptor, expression of F1 pili and virulence for chickens) were investigated to look for correlations with the obtained sequences. The sequence analysis demonstrated that FimH is highly conserved among all investigated APEC strains (>99% homology), whereas the major subunit FimA is less conserved, presenting 6 variable regions distributed along the protein. A hydrophilicity analysis suggested several variable domains of FimA to be potential epitopes. We were able to classify the investigated strains into three main groups, on the basis of the amino-acid sequences of the variable regions. This grouping was consistent throughout all variable regions and was independent of serotype, leading to an improved classification of the F1 pili. No correlation was found between the fimH and fimA sequences and the following parameters: avian species, organ of isolation, serotype, presence of aerobactin receptor and virulence for chickens. This study elucidated the molecular structure and the degree of conservation of FimH and FimA among various avian pathogenic E. coli strains.
Vandemaele
Fréderic
F
Laboratory of Physiology and Immunology of Domestic Animals, Catholic University Leuven, Kasteelpark Arenberg 30, 3001 Leuven, Belgium. Frederic.vandemaele@agr.kuleuven.ac.be
Vandekerchove
Dominique
D
Vereecken
Monita
M
Derijcke
Jef
J
Dho-Moulin
Maryvonne
M
Goddeeris
Bruno Maria
BM
D018830:Adhesins, Escherichia coli / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D002645:Chickens / Q000382:microbiology; D017124:Conserved Sequence / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D036781:Fimbriae Proteins / Q000235:genetics*; D010861:Fimbriae, Bacterial / Q000502:physiology; D014644:Genetic Variation / Q000235:genetics*; D008969:Molecular Sequence Data; D011201:Poultry Diseases / Q000382:microbiology*; D016415:Sequence Alignment; D014774:Virulence
D018830:Adhesins, Escherichia coli; D001425:Bacterial Outer Membrane Proteins; C034506:aerobactin receptor; C058769:fimH protein, E coli; C063055:fimbrillin; D036781:Fimbriae Proteins