Comparison of expression patterns between CREB family transcription factor OASIS and proteoglycan core protein genes during murine tooth development.
The transcription factor OASIS gene, which encodes for a CREB/ATF family member, is specifically expressed in the salivary gland, the cartilage and the tooth germs of the mouse embryo. In the present study, the expression patterns were compared between OASIS mRNA and major vertebrate proteoglycans, which might be the downstream genes of OASIS in the tooth germs of mouse first mandibular molars, through in situ hybridization histochemistry. OASIS mRNA expression was observed in the inner enamel epithelium during the cap and bell stages (E14.5-E18.5) in the preodontoblasts during differentiation stage (E18.5-P0) and in the differentiating odontoblasts during the early secretory stage (P2.5-P4.5). Proteoglycans (versican, decorin, biglycan, glypican, syndecan-1, and syndecan-3) were expressed in the tooth germs in various patterns. Decorin, biglycan, syndecan-1 and syndecan-3 showed gene expressions overlapping with OASIS. Especially the expression pattern of decorin and syndecan-3 coincided temporally and spatially exactly with that of OASIS. These results suggest that the OASIS gene might be related to proteoglycan expression and may play an important role in the differentiation of the odontoblast and cells in inner enamel epithelium.
Hikake
Tsuyoshi
T
Department of Cell Science, Institute of Biomedical Sciences, Fukushima Medical University School of Medicine, 960-1295 Fukushima, Japan.
Mori
Tetsuji
T
Iseki
Ken
K
Hagino
Seita
S
Zhang
Yuxiang
Y
Takagi
Hiromi
H
Yokoya
Sachihiko
S
Wanaka
Akio
A
D053674:Aggrecans; D000818:Animals; D017362:Cyclic AMP Response Element-Binding Protein / Q000235:genetics / Q000378:metabolism*; D016326:Extracellular Matrix Proteins; D018507:Gene Expression Regulation, Developmental; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D017403:In Situ Hybridization; D037181:Lectins, C-Type; D051379:Mice; D008813:Mice, Inbred ICR; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009805:Odontogenesis / Q000235:genetics; D011509:Proteoglycans; D012333:RNA, Messenger / Q000378:metabolism; D013045:Species Specificity; D014083:Tooth Germ / Q000196:embryology* / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
C508320:Acan protein, mouse; D053674:Aggrecans; C119923:Creb3l1 protein, mouse; D017362:Cyclic AMP Response Element-Binding Protein; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D037181:Lectins, C-Type; D009419:Nerve Tissue Proteins; D011509:Proteoglycans; D012333:RNA, Messenger; D014157:Transcription Factors