Circadian and light-induced expression of luciferase in Neurospora crassa.
We have constructed a plasmid vector for expressing firefly luciferase in Neurospora crassa under control of the light- and clock-regulated ccg-2 (eas) promoter. The sequence of the luciferase gene in the vector has been modified to reflect the N. crassa codon bias. Both light-induced activity and circadian activity are demonstrated. Expression of luciferase in strains carrying mutant frequency alleles shows appropriate period length alterations. These data demonstrate that luciferase is a sensitive reporter of gene expression in N. crassa. Our results also show that the modified luciferase is expressed in Aspergillus nidulans.
Morgan
Louis W
LW
Department of Biology, Texas A&M University, College Station, TX 77843-3258, USA.
Greene
Andrew V
AV
Bell-Pedersen
Deborah
D
D001233:Aspergillus nidulans / Q000201:enzymology / Q000235:genetics; D001483:Base Sequence; D002940:Circadian Rhythm; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005822:Genetic Vectors; D008027:Light; D008156:Luciferases / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000201:enzymology / Q000235:genetics; D010957:Plasmids
D005656:Fungal Proteins; D008156:Luciferases