[Effect of different storage methods on the viability of Halobacterium salinarum].
It has been established that the studied halobacteria are cryolabile and sensitive to the sharp change in osmotic pressure of the environment. Optimal temperature of their storage is +20 degrees C for suspension of native cells and +4 degrees C for the cells in anabiotic state. Their storage medium should have optimal osmotic pressure and contain NaCl and K+, Mg+ ions. It is recommended to keep halobacteria during one year in a form of suspension in nutrient medium, and during at least 4 years in lyophilized state. Other long-term storage methods: L-drying, freezing at low temperatures, are not recommended for halobacteria.
Holovach
T M
TM
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, 154 Zabolotny St., Kyiv, 03143, Ukraine.
Sudenko
V I
VI
D003470:Culture Media / Q000378:metabolism; D005612:Freeze Drying; D019617:Halobacterium salinarum / Q000378:metabolism / Q000502:physiology*; D008274:Magnesium / Q000378:metabolism; D009997:Osmotic Pressure; D011188:Potassium / Q000378:metabolism; D011309:Preservation, Biological / Q000379:methods*; D012965:Sodium Chloride / Q000378:metabolism; D013535:Suspensions; D013696:Temperature; D013997:Time Factors
D003470:Culture Media; D013535:Suspensions; D012965:Sodium Chloride; D008274:Magnesium; D011188:Potassium