title: Quantitative detection of Chlamydia psittaci and C. pecorum by high-sensitivity real-time PCR reveals high prevalence of vaginal infection in cattle.
abstract: Bovine vaginal cytobrush specimens were analyzed for the presence of Chlamydia spp. by a high-sensitivity, high-specificity quantitative PCR. The 53% prevalence of low-level Chlamydia psittaci and C. pecorum genital infection detected in virgin heifers suggests predominantely extragenital transmission of Chlamydia in cattle and conforms to the high seroprevalence of anti-Chlamydia antibodies.
authors.affiliation: Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, Alabama 36849-5519, USA.
authors.forename: Fred J
authors.initials: FJ
authors.lastname: DeGraves
authors.forename: Dongya
authors.initials: D
authors.lastname: Gao
authors.forename: Hans-Robert
authors.initials: HR
authors.lastname: Hehnen
authors.forename: Tobias
authors.initials: T
authors.lastname: Schlapp
authors.forename: Bernhard
authors.initials: B
authors.lastname: Kaltenboeck
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000382:microbiology; D002689:Chlamydia / Q000235:genetics / Q000302:isolation & purification*; D002690:Chlamydia Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary; D002691:Chlamydophila psittaci / Q000235:genetics / Q000302:isolation & purification*; D004275:DNA, Ribosomal / Q000032:analysis; D016326:Extracellular Matrix Proteins / Q000235:genetics; D005260:Female; D029941:Fish Proteins; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D008565:Membrane Proteins; D016133:Polymerase Chain Reaction / Q000379:methods*; D015995:Prevalence; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012680:Sensitivity and Specificity; D014627:Vaginitis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D004275:DNA, Ribosomal; D016326:Extracellular Matrix Proteins; D029941:Fish Proteins; D008565:Membrane Proteins; D012336:RNA, Ribosomal, 16S