title: Production of the glycopeptide antibiotic A40926 by Nonomuraea sp. ATCC 39727: influence of medium composition in batch fermentation.
abstract: Nonomuraea sp. ATCC 39727 is a novel actinomycete species and the producer of A40926, a glycopeptide antibiotic structurally similar to teichoplanin. In the present study, a defined minimal medium was designed for Nonomuraea fermentation. The influence of initial phosphate, glucose and ammonium concentrations on antibiotic productivity was investigated in batch fermentation and the effect of glucose limitation was studied in fed-batch fermentation. It was found that low initial concentrations of phosphate and ammonium are beneficial for A40926 production and that productivity is not enhanced during glucose limitation. Furthermore, the initiation of A40926 production was not governed by residual ammonium and phosphate concentrations, although the level of these nutrients strongly influenced A40926 production rates and final titers.
authors.affiliation: Center for Process Biotechnology, Biocentrum-DTU, Building 223, Søltofts plads, 2800, Lyngby, Denmark.
authors.forename: Nina
authors.initials: N
authors.lastname: Gunnarsson
authors.forename: Per
authors.initials: P
authors.lastname: Bruheim
authors.forename: Jens
authors.initials: J
authors.lastname: Nielsen
mesh_terms: D039903:Actinobacteria / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006020:Glycopeptides; D010710:Phosphates / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D017334:Teicoplanin / Q000031:analogs & derivatives
chemical_list: C054981:A 40926; D000900:Anti-Bacterial Agents; D003470:Culture Media; D006020:Glycopeptides; D010710:Phosphates; D000644:Quaternary Ammonium Compounds; D017334:Teicoplanin; D005947:Glucose