title: [Effects of G894T mutation in the endothelial nitric oxide synthase gene on blood pressure].
abstract: OBJECTIVE
  To analyze the association between G894T (Glu298Asp) mutation at exon 7 in the endothelial nitric oxide synthase gene and essential hypertension.
  
  
  METHODS
  One hundred and sixteen essential hypertensives without taking hypertensive medication and 136 normotensives screened from health workers in a steel factory were selected as subjects in this study. Polymerase chain reaction (PCR) and Ban II restriction enzyme digestion were performed to detect the G894T mutation.
  
  
  RESULTS
  G894T mutation was significantly associated with essential hypertension. The T allele frequency in essential hypertensive group was significantly higher than that in normotensive group (16.0% versus 8.8%, P = 0.019, OR = 1.96, 95% CI: 1.14 - 3.37). The levels of systolic blood pressure and diastolic blood pressure in the G894T mutant genotypes were all significantly elevated in hypertensive, normotensive, and the total subjects (P < 0.05). After adjusting factors as age, sex, smoking, alcohol drinking, body mass index, triglyceride, serum total cholesterol, serum high density lipoprotein cholesterol by analysis of multiple covariance, significant positive effect of the G894T mutant genotypes on blood pressure in the total subjects (P < 0.01) was noticed.
  
  
  CONCLUSIONS
  This study suggested that the G894T mutation in the endothelial nitric oxide synthase gene might serve as a major risk factor of essential hypertension in this study population.
authors.affiliation: Department of Epidemiology, College of Public Health, Shandong University, Jinan 250012, China.
authors.forename: Chong-qi
authors.initials: CQ
authors.lastname: Jia
authors.forename: Zhong-tang
authors.initials: ZT
authors.lastname: Zhao
authors.forename: Li-hua
authors.initials: LH
authors.lastname: Wang
authors.forename: Feng-rong
authors.initials: FR
authors.lastname: Hao
authors.forename: Yue-qiu
authors.initials: YQ
authors.lastname: Feng
authors.forename: Shu-mei
authors.initials: SM
authors.lastname: Wang
authors.forename: Xiao-fei
authors.initials: XF
authors.lastname: Xu
authors.forename: Cun-xian
authors.initials: CX
authors.lastname: Jia
mesh_terms: D000328:Adult; D000483:Alleles; D001794:Blood Pressure / Q000235:genetics; D002681:China / Q000453:epidemiology; D005091:Exons; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006801:Humans; D006973:Hypertension / Q000201:enzymology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D019001:Nitric Oxide Synthase / Q000235:genetics*; D052250:Nitric Oxide Synthase Type III; D017354:Point Mutation; D012150:Polymorphism, Restriction Fragment Length; D012307:Risk Factors
chemical_list: C496314:NOS3 protein, human; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III