Gene expression study of Saccharomyces cerevisiae with the Agilent 2100 bioanalyser.
This study explores the restriction display-polymerase chain reaction (RD-PCR) application of a new chip-based nucleic acid analysis system (Agilent 2100 bioanalyser) in a gene differential expression study. Total RNAs is extracted from Saccharomyces cerevisiae, double-stranded complementary DNA (cDNA) is synthesised by reverse transcription from the purified messenger RNA (mRNA), RD-PCR conducted to obtain the cDNA fragments and bioanalyser and agarose gel electrophoresis compared for the analysis of RD-PCR products. The bioanalyser proved to be faster and more sensitive in separating and detecting gene fragments, and was also able to compare different gene fragments quantitatively. Using this technology, comparison of several differential gene fragments is performed.
Liu
Cui-Hua
CH
Institute of Molecular Biology, First Military Medical University, Guangzhou 510515, China.
Ma
Wen-Li
WL
Shi
Rong
R
Zhang
Bao
B
Ou
Yang-Qian
YQ
Zheng
Wen-Ling
WL
D004247:DNA / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D015870:Gene Expression / Q000235:genetics*; D006360:Heat-Shock Proteins / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000295:instrumentation*; D015183:Restriction Mapping / Q000295:instrumentation; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics*; D012680:Sensitivity and Specificity
D006360:Heat-Shock Proteins; D004247:DNA