title: Improvement of industry-applied rifamycin B-producing strain, Amycolatopsis mediterranei, by rational screening.
abstract: An industrially applied rifamycin B-producing strain, Amycolatopsis mediterranei XC 1-02, was used for further screening. A special mutation and screening procedure was adopted to select a strain, which can alleviate the inhibition caused by both aromatic amino acid and p-hydroxybenzoic acid in the pathway of rifamycin B biosynthesis as well as enhance the production of propionate, one of the precursors of rifamycin B biosynthesis. By the above methods, a strain A. mediterranei XC 9-25 was obtained, and its rifamycin B productivity in shaking flask reaches 10 g/L, which is 2.38 times higher than that of the ancestral strain XC 1-02. The productivity of rifamycin B fed-batch fermentation in 60000 L fermentor with A. mediterranei XC 9-25 reached 19.11 g/L.
authors.affiliation: Institute of Bioengineering, College of Materials Science and Chemical Engineering, Zhejiang University, Hangzhou 310027, P. R. China.
authors.forename: Zhi Hua
authors.initials: ZH
authors.lastname: Jin
authors.forename: Jiang Ping
authors.initials: JP
authors.lastname: Lin
authors.forename: Zhi Nan
authors.initials: ZN
authors.lastname: Xu
authors.forename: Pei Lin
authors.initials: PL
authors.lastname: Cen
mesh_terms: D000192:Actinomycetales / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D024322:Amino Acids, Aromatic / Q000494:pharmacology; D001709:Biotechnology / Q000379:methods*; D003470:Culture Media; D005285:Fermentation; D009154:Mutation; D010226:Parabens / Q000494:pharmacology; D011422:Propionates / Q000378:metabolism; D012294:Rifamycins / Q000096:biosynthesis*; D014364:Tryptophan / Q000494:pharmacology; D014466:Ultraviolet Rays
chemical_list: D024322:Amino Acids, Aromatic; D003470:Culture Media; D010226:Parabens; D011422:Propionates; D012294:Rifamycins; C033974:rifamycin B; D014364:Tryptophan; C038193:4-hydroxybenzoic acid