Correlation of in vivo photosensitizer fluorescence and photodynamic-therapy-induced depth of necrosis in a murine tumor model.
We compared light-induced fluorescence (LIF) to nominal injected drug dose for predicting the depth of necrosis response to photodynamic therapy (PDT) in a murine tumor model. Mice were implanted with radiation-induced fibrosarcoma (RIF) and were injected with 0, 5, or 10 mg/kg Photofrin. 630-nm light (30 J/cm(2), 75 mW/cm(2)) was delivered to the tumor after 24 hours. Fluorescence emission (lambda(excitation)=545 nm, lambda( emission)=628 nm) from the tumor was measured. The LIF data had less scatter than injected drug dose, and was found to be at least as good as an injected drug dose for predicting the depth of necrosis after PDT. Our observations provide further evidence that fluorescence spectroscopy can be used to quantify tissue photosensitizer uptake and to predict PDT tissue damage.
Cheung
Rex
R
University of Pennsylvania, Department of Physics and Astronomy, Philadelphia, Pennsylvania 19104, USA.
Solonenko
Michael
M
Busch
Theresa M
TM
Del Piero
Fabio
F
Putt
M E
ME
Hahn
Stephen M
SM
Yodh
Arjun G
AG
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000493:pharmacokinetics; D017323:Dihematoporphyrin Ether / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005354:Fibrosarcoma / Q000175:diagnosis / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology*; D007275:Injections, Intravenous; D051379:Mice; D008809:Mice, Inbred C3H; D009336:Necrosis; D009368:Neoplasm Transplantation; D009381:Neoplasms, Radiation-Induced; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000008:administration & dosage / Q000493:pharmacokinetics; D011237:Predictive Value of Tests; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013223:Statistics as Topic; D014018:Tissue Distribution; D016896:Treatment Outcome
D000970:Antineoplastic Agents; D017319:Photosensitizing Agents; D017323:Dihematoporphyrin Ether