title: Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis.
abstract: The complete genome sequence of Enterococcus faecalis V583, a vancomycin-resistant clinical isolate, revealed that more than a quarter of the genome consists of probable mobile or foreign DNA. One of the predicted mobile elements is a previously unknown vanB vancomycin-resistance conjugative transposon. Three plasmids were identified, including two pheromone-sensing conjugative plasmids, one encoding a previously undescribed pheromone inhibitor. The apparent propensity for the incorporation of mobile elements probably contributed to the rapid acquisition and dissemination of drug resistance in the enterococci.
authors.affiliation: Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA. ipaulsen@tigr.org
authors.forename: I T
authors.initials: IT
authors.lastname: Paulsen
authors.forename: L
authors.initials: L
authors.lastname: Banerjei
authors.forename: G S A
authors.initials: GS
authors.lastname: Myers
authors.forename: K E
authors.initials: KE
authors.lastname: Nelson
authors.forename: R
authors.initials: R
authors.lastname: Seshadri
authors.forename: T D
authors.initials: TD
authors.lastname: Read
authors.forename: D E
authors.initials: DE
authors.lastname: Fouts
authors.forename: J A
authors.initials: JA
authors.lastname: Eisen
authors.forename: S R
authors.initials: SR
authors.lastname: Gill
authors.forename: J F
authors.initials: JF
authors.lastname: Heidelberg
authors.forename: H
authors.initials: H
authors.lastname: Tettelin
authors.forename: R J
authors.initials: RJ
authors.lastname: Dodson
authors.forename: L
authors.initials: L
authors.lastname: Umayam
authors.forename: L
authors.initials: L
authors.lastname: Brinkac
authors.forename: M
authors.initials: M
authors.lastname: Beanan
authors.forename: S
authors.initials: S
authors.lastname: Daugherty
authors.forename: R T
authors.initials: RT
authors.lastname: DeBoy
authors.forename: S
authors.initials: S
authors.lastname: Durkin
authors.forename: J
authors.initials: J
authors.lastname: Kolonay
authors.forename: R
authors.initials: R
authors.lastname: Madupu
authors.forename: W
authors.initials: W
authors.lastname: Nelson
authors.forename: J
authors.initials: J
authors.lastname: Vamathevan
authors.forename: B
authors.initials: B
authors.lastname: Tran
authors.forename: J
authors.initials: J
authors.lastname: Upton
authors.forename: T
authors.initials: T
authors.lastname: Hansen
authors.forename: J
authors.initials: J
authors.lastname: Shetty
authors.forename: H
authors.initials: H
authors.lastname: Khouri
authors.forename: T
authors.initials: T
authors.lastname: Utterback
authors.forename: D
authors.initials: D
authors.lastname: Radune
authors.forename: K A
authors.initials: KA
authors.lastname: Ketchum
authors.forename: B A
authors.initials: BA
authors.lastname: Dougherty
authors.forename: C M
authors.initials: CM
authors.lastname: Fraser
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics; D005075:Biological Evolution; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D002876:Chromosomes, Bacterial / Q000235:genetics; D003227:Conjugation, Genetic; D017124:Conserved Sequence; D004251:DNA Transposable Elements; D004064:Digestive System / Q000382:microbiology; D024901:Drug Resistance, Multiple, Bacterial; D013293:Enterococcus faecalis / Q000187:drug effects / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D022761:Gene Transfer, Horizontal; D016680:Genome, Bacterial; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology; D006801:Humans; D020071:Interspersed Repetitive Sequences; D008242:Lysogeny; D016366:Open Reading Frames; D018384:Oxidative Stress; D010957:Plasmids; D017422:Sequence Analysis, DNA; D026801:Synteny; D020713:Vancomycin Resistance / Q000235:genetics*; D014774:Virulence / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics
chemical_list: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D002352:Carrier Proteins; D004251:DNA Transposable Elements; D037521:Virulence Factors