title: [Screening and application of human-derived HBsAg bacteriophage single chain antibody in clinical diagnosis].
abstract: OBJECTIVE
  To identify human single chain Fv antibody (ScFv) against hepatitis B viral surface antigen.
  
  
  METHODS
  The recombinant phages were panned by HBsAg which was coated in a microtiter plate, after five rounds of biopanning, 56 phage clones were identified specific to HBsAg. The specificity of ScFv was evaluated by ELISA and immunohistochemistry, respectively.
  
  
  RESULTS
  The data of HB sAg-ScFv DNA digestion and DNA sequencing showed that the ScFv gene is composed of 750 bp. ELISA and immunohistochemistry demonstrated that the human single chain Fv antibody against hepatitis B surface antigen has a specific combination character with hepatitis B surface antigen of different sources and paraffin-embedded patients tissue specimens, it did not react with normal liver tissue and HCV.
  
  
  CONCLUSIONS
  The application of HBsAg specific ScFv in immunohistochemistry was successfully achieved.
authors.affiliation: Gene Therapy Research Centre, Institute of Infectious Diseases, The 302nd Hospital of PLA, Beijing 100039, China.
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Zhong
authors.forename: Jun
authors.initials: J
authors.lastname: Cheng
authors.forename: Shuangshuang
authors.initials: S
authors.lastname: Shi
authors.forename: Jingmin
authors.initials: J
authors.lastname: Zhao
authors.forename: Gang
authors.initials: G
authors.lastname: Wang
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Xia
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Tian
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Lingxia
authors.initials: L
authors.lastname: Zhang
mesh_terms: D001435:Bacteriophages / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D019694:Hepatitis B, Chronic / Q000175:diagnosis*; D006801:Humans; D019151:Peptide Library
chemical_list: D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D019151:Peptide Library