Cell-adhesion and morphological changes are not sufficient to support anchorage-dependent cell growth via non-integrin-mediated attachment.
Cell-adhesion and spread are important for cell survival. Although extensive studies have suggested several potential mechanisms of action, it is not yet clear how important cell-morphological change per se contributes to the cell-surviving signal. We employed a non-integrin-mediated cell-adhesion system to explore this question. BHK-Japanese encephalitis virus (JEV) cells (BHK21 cells that are persistently infected with JEV) express a large amount of JEV-envelope protein (JEV E) on their surfaces, and can attach and form pseudopodia on the anti-JEV E antibody-coated substrates. However, cells that adhered on the antibody substrate underwent a caspase-3-mediated apoptosis together with a down-regulation of mitogen-activated protein kinase activity within 20 h after adhesion, which indicates that viral-protein-mediated cell-adhesion and cell-spread are not sufficient for supporting cell survival. This provides a different perspective for the study of the relationships between the cell-morphological change and the cell-survival signal.
Chang
Hsin-Hou
HH
Institute of Molecular and Cellular Biology, Tzu-Chi University, Hualien 970, Taiwan, ROC. hhchang@mail.tcu.edu.tw
Kau
Jyh-Hwa
JH
Lo
Szecheng J
SJ
Sun
Der-Shan
DS
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002999:Clone Cells / Q000166:cytology / Q000187:drug effects / Q000821:virology; D004664:Encephalitis Virus, Japanese / Q000254:growth & development* / Q000378:metabolism; D005353:Fibronectins / Q000494:pharmacology; D005434:Flow Cytometry; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000276:immunology / Q000378:metabolism; D017361:Viral Nonstructural Proteins / Q000276:immunology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D005353:Fibronectins; D014759:Viral Envelope Proteins; D017361:Viral Nonstructural Proteins; D020928:Mitogen-Activated Protein Kinases; D053148:Caspase 3; D020169:Caspases