[Array technology in skin pharmacology and allergology].
Because of their variable application, microarrays are currently used in different areas of research and development, such as skin pharmacology and allergology. Microarrays are plane carriers, on whose surface a variety of known DNA-molecules and proteins were immobilised. Transcripts can be detected by cDNA- and oligonucleotid-arrays and proteins of activated genes can be discovered using antibody microarrays. Detection of allergen-specific IgE from human serum can be performed using allergen chips. Since many details of the molecular mechanism and pathogenesis of skin cancer and inflammatory skin diseases and the effect of xenobiotics on cells of the human skin are still not known, array-technologies are a powerful tool to identify novel marker genes and offer the possibility of develop new therapeutic strategies as well as prognosis- and diagnosis-systems.
Baron
J M
JM
Hautklinik, Universitätsklinikum der RWTH Aachen. JensMalte.Baron@post.rwth-aachen.de
Merk
H F
HF
Heise
R
R
D000485:Allergens / Q000235:genetics*; D000486:Allergy and Immunology; D000906:Antibodies / Q000235:genetics*; D001345:Autoradiography; D018076:DNA, Complementary; D005453:Fluorescence; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D020411:Oligonucleotide Array Sequence Analysis; D010600:Pharmacology; D011379:Prognosis; D040081:Protein Array Analysis; D040901:Proteomics; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger; D012106:Research; D012867:Skin / Q000187:drug effects*; D012871:Skin Diseases / Q000175:diagnosis / Q000235:genetics* / Q000628:therapy; D014158:Transcription, Genetic; D015262:Xenobiotics / Q000494:pharmacology
D000485:Allergens; D000906:Antibodies; D018076:DNA, Complementary; D012333:RNA, Messenger; D015262:Xenobiotics; D007073:Immunoglobulin E; D012313:RNA