Lignocellulose degradation by Pleurotus ostreatus in the presence of cadmium.
Activities of cellulolytic and hemicellulolytic enzymes endo-1,4-beta-glucanase, exo-1,4-beta-glucanase, 1,4-beta-glucosidase, endo-1,4-beta-xylanase, 1,4-beta-xylosidase and 1,4-beta-mannosidase and ligninolytic enzymes Mn-peroxidase and laccase were detected during the growth of the white-rot fungus Pleurotus ostreatus on wheat straw in the presence and absence of cadmium. The loss of substrate dry weight and Mn-peroxidase activity decreased with increasing Cd concentration, whereas the activities of endo-1,4-beta-glucanase, 1,4-beta-glucosidase and laccase were highly increased in the presence of metal. The onset of hemicellulose-degrading enzyme activity was delayed in the presence of cadmium. The degradation of a model synthetic dye Poly B-411 did not correspond to the activities of ligninolytic enzymes. This is the first report about 1,4-beta-mannosidase in P. ostreatus.
Baldrian
Petr
P
Laboratory of Biochemistry of the Wood-rotting Fungi, Institute of Microbiology AS CR, Víndeoská 1083, 14220 Prague 4, Czech Republic. baldrian@biomed.cas.cz
Gabriel
Jirí
J
D001673:Biodegradation, Environmental; D002104:Cadmium / Q000494:pharmacology*; D002480:Cellulase; D002482:Cellulose / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism; D042845:Laccase; D008031:Lignin / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D020076:Pleurotus / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism
D002104:Cadmium; C036909:lignocellulose; D002482:Cellulose; D008031:Lignin; D010088:Oxidoreductases; D042845:Laccase; D009195:Peroxidase; D006026:Glycoside Hydrolases; D002480:Cellulase; C019826:carboxymethylcellulase