title: Inhibition study of rhodanese by means of electrophoretically mediated microanalysis.
abstract: A combination of the electrophoretically mediated microanalysis methodology with a partial filling technique was applied for the inhibition study of bovine liver rhodanese by 2-oxoglutarate. In this set-up, part of the capillary is filled with the best buffer for the enzymatic reaction, while the rest of the capillary is filled with the optimal background electrolyte for separation of substrates and products. The estimated value of K1 for 2-oxoglutarate was 3.62 x 10(-4) +/- 1.43 x 10(-4) M with respect to cyanide and 1.40 x 10(-3) +/- 1.60 x 10(-4) M with respect to thiosulfate. In addition, the type of inhibition was also evaluated. The findings of 2-oxoglutarate as the competitive inhibitor with respect to cyanide and as the uncompetitive inhibitor with respect to thiosulfate are in accordance with previous literature data.
authors.affiliation: Department of Biochemistry, Faculty of Science, Masaryk University, Kotlárská 2, 611 37 Brno, Czech Republic.
authors.forename: Sona
authors.initials: S
authors.lastname: Nováková
authors.forename: Magdaléna
authors.initials: M
authors.lastname: Telnarová
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Glatz
mesh_terms: D004586:Electrophoresis / Q000379:methods*; D013379:Substrate Specificity; D013884:Thiosulfate Sulfurtransferase / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D013884:Thiosulfate Sulfurtransferase