Post-ischemic cyclooxygenase-2 expression is regulated by the extent of cerebral blood flow reduction in non-human primates.
We determined whether up to 24 h of ischemia could induce the expression of cyclooxygenase-2 (COX-2) in the brain of nonhuman primates. Randomized animals were subjected to either a 2 h ischemia (group II; n=3) or a 24 h ischemia (group III; n=3). Three animals in group I served as controls. In group III, regional cerebral blood flow (CBF) and the cerebral glucose metabolic rate (CMRglc) were evaluated using positron emission tomography. Upregulation of COX-2 mRNA expression was observed after 2 h of ischemia, but disappeared by 24 h in the ischemic temporal cortex, in which both CMRglc and CBF were markedly reduced. In the ischemic parietal cortex, where CMRglc was preserved, COX-2 expression persisted even 24 h after ischemia. This study is the first to demonstrate neuronal COX-2 induction within potentially viable hypoperfused brain areas in nonhuman primates.
Yokota
Chiaki
C
Cerebrovascular Laboratory, National Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Suita 565-8565, Japan. cyokota@ri.ncvc.go.jp
Kuge
Yuji
Y
Inoue
Hiroyasu
H
Tagaya
Masafumi
M
Kito
Go
G
Susumu
Teruo
T
Tamaki
Nagara
N
Minematsu
Kazuo
K
D000818:Animals; D001783:Blood Flow Velocity / Q000502:physiology; D002545:Brain Ischemia / Q000201:enzymology* / Q000503:physiopathology; D002560:Cerebrovascular Circulation / Q000502:physiology*; D051546:Cyclooxygenase 2; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D007527:Isoenzymes / Q000096:biosynthesis*; D008252:Macaca fascicularis; D008297:Male; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis; D013997:Time Factors
D007527:Isoenzymes; D012333:RNA, Messenger; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases