title: Interleukin-6 receptor expression and localization after transient global ischemia in gerbil hippocampus.
abstract: Ischemia results in increased interleukin-6 (IL-6) expression in the brain. To prove a connection between IL-6 upregulation and IL-6 receptor (IL-6R) expression following ischemia, we analyzed cell-type specific expression changes of IL-6R using transient global ischemia in the gerbil as a model. In sham operated animals, IL-6R mRNA and protein were mainly detected in hippocampal pyramidal cells and interneurons. After ischemia, IL-6R was expressed in neurons but there was no increase during the peak IL-6 expression. Neuronal IL-6R mRNA and protein decreased in parallel with pyramidal cell death, starting 2 days after ischemia. Double-labeling experiments revealed that in postischemic hippocampus IL-6R was not present in GFAP-reactive astrocytes but that the surviving parvalbumin containing interneurons expressed IL-6R mRNA.
authors.affiliation: Institute of Anatomy, Basel University, Pestalozzistrasse 20, CH-4056 Basel, Switzerland. florence.vollenweider@unibas.ch
authors.forename: Florence
authors.initials: F
authors.lastname: Vollenweider
authors.forename: Martina
authors.initials: M
authors.lastname: Herrmann
authors.forename: Uwe
authors.initials: U
authors.lastname: Otten
authors.forename: Cordula
authors.initials: C
authors.lastname: Nitsch
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation / Q000502:physiology; D005849:Gerbillinae; D006624:Hippocampus / Q000378:metabolism*; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis; D019947:Receptors, Interleukin-6 / Q000096:biosynthesis*
chemical_list: D012333:RNA, Messenger; D019947:Receptors, Interleukin-6