A metal nebulizer capillary electrophoresis/Fourier transform infrared spectrometric interface.
A capillary electrophoretic (CE) system has been successfully interfaced to a Fourier transform infrared spectrometer. The advantage of such an interface is that analytes may be detected and often unequivocally identified without analyte derivatization. The interface consists of a stainless steel tube in which the CE capillary is placed and the two are held in contact with the use of a metal tee. A solvent elimination approach is used with the interface, so that analytes are deposited onto an infrared transparent window, that is, CaF2, and measured with the use of an infrared microscope. A critical component of this design is to provide an electrical connection at the end of the CE column to permit stable separations that allow for efficient transport of the sample onto the window. The interface produces an aerosol that is directed at the surface of the infrared transparent window. The use of a volatile electrolyte, along with the flow of helium, allows for partial evaporation of the electrolyte in flight and complete evaporation of the solvent and electrolyte on the surface of the window to produce a "dry", or neat, analyte deposit.
Todebush
Richard A
RA
University of Georgia, Department of Chemistry, Athens, Georgia 30602-2556, USA.
He
Lin-Tao
LT
de Haseth
James A
JA
D000116:Acetylgalactosamine / Q000032:analysis; D001241:Aspirin / Q000032:analysis; D002110:Caffeine / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D004867:Equipment Design; D008722:Methods; D009330:Nebulizers and Vaporizers; D000644:Quaternary Ammonium Compounds / Q000032:analysis; D017550:Spectroscopy, Fourier Transform Infrared / Q000295:instrumentation*
D000644:Quaternary Ammonium Compounds; D002110:Caffeine; D000116:Acetylgalactosamine; D001241:Aspirin