Activation of the aryl hydrocarbon receptor by some vegetable constituents determined using in vitro reporter gene assay.
The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that mediates the biological action of many aromatic environmental pollutants. In this study, we investigated the activation of the AhR by some vegetable constituents using the AhR-based bioassay for dioxins, i.e., the chemical activated luciferase gene expression (CALUX) assay. Ninety-five vegetable constituents, including flavonoids, tannins, saponins, and terpenes, were tested in vitro. Among them, isoflavones such as daidzein, resveratrol having a stilbene structure, and some flavonoids such as naringenin, hesperetin, and baicalein showed AhR activation.
Amakura
Yoshiaki
Y
Division of Foods, National Institute of Health Sciences, Tokyo, Japan. amakura@nihs.go.jp
Tsutsumi
Tomoaki
T
Nakamura
Masafumi
M
Kitagawa
Hiroko
H
Fujino
Junko
J
Sasaki
Kumiko
K
Toyoda
Masatake
M
Yoshida
Takashi
T
Maitani
Tamio
T
D000818:Animals; D045744:Cell Line, Tumor; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000502:physiology; D017930:Genes, Reporter / Q000502:physiology*; D007529:Isoflavones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D018336:Receptors, Aryl Hydrocarbon / Q000819:agonists / Q000235:genetics / Q000378:metabolism*; D014675:Vegetables
D007529:Isoflavones; D018336:Receptors, Aryl Hydrocarbon; D008156:Luciferases