[Change of expression of cell/organs defense genes in brain tissue of rats with stroke-like episodes induced by complex environmental factors].
OBJECTIVE
To investigate the effect of environmental risk factors on the development of stroke.


METHODS
With the use of cold-stimuli plus high-salt intake as environmental risk factors, a hypertension model with the complication of stroke was established in rats, then, a new technique, suppression subtractive hybridization (SSH), was used to identify the differential genes which specifically expressed in total cerebrum tissue of rat in each group. Comparison was made between control group and stroke group.


RESULTS
By the application of SSH, a total of 576 clones were generated in this study from two subtractive libraries, among them 456 clones were usable and were analyzed. Genes for cell/organs defense were down-regulated in stroke group and metabolism transcripts were shown to be up-regulated (P<0.01).


CONCLUSIONS
Cell/organs defense genes may play important roles in the development of stroke. The above findings suggested that environmental risk factors could genetically alter individual sensitivity to stroke.
Wang
Xianmei
X
Department of Cardiology, Kunming General Hospital, Chengdu Military Area, Kunming, Yunan, 650032 P. R. China. wangxianmei89@yahoo.com
Yan
Rui
R
Yang
Lixia
L
Qi
Feng
F
Zhu
Shanjun
S
Zhao
Bin
B
Zhang
Qian
Q
Hui
Rutai
R
Guo
Chuanming
C
Wei
Ling
L
D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D002455:Cell Division / Q000235:genetics; D003080:Cold Temperature; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D020224:Expressed Sequence Tags; D005786:Gene Expression Regulation / Q000187:drug effects; D007113:Immunity, Innate / Q000235:genetics; D008297:Male; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017422:Sequence Analysis, DNA; D015398:Signal Transduction / Q000235:genetics; D017673:Sodium Chloride, Dietary / Q000008:administration & dosage; D020521:Stroke / Q000235:genetics*
D018076:DNA, Complementary; D012333:RNA, Messenger; D017673:Sodium Chloride, Dietary