Standardisation of cell lines.
Standardisation of cell-based assays is significantly enhanced through the preparation and quality control of master and working cell banks. Validation of the cell substrate for use in an assay must address not only the assay conditions but also aspects such as culture passage level, pre-assay preparation and seeding of cells and pre-use validation of "critical reagents".
Stacey
G N
GN
NIBSC, Potters Bar, Herts., UK. gstacey@nibsc.ac.uk
D000818:Animals; D001419:Bacteria / Q000378:metabolism; D002460:Cell Line; D005658:Fungi / Q000378:metabolism; D005838:Genotype; D006801:Humans; D010641:Phenotype; D017096:Prion Diseases; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D014780:Viruses / Q000378:metabolism