Enterobacterial repetitive intergenic consensus 1R PCR assay for detection of Raoultella sp. isolates among strains identified as Klebsiella oxytoca in the clinical laboratory.
The enterobacterial repetitive intergenic consensus 1R PCR method, which provided recognizable profiles for reference strains of the three species of Raoultella and the two genetic groups of Klebsiella oxytoca, was applied to 19 clinical isolates identified as K. oxytoca. By this method, as confirmed by species-specific gene sequencing, two Raoultella ornithinolytica and two unclassifiable K. oxytoca isolates were identified.
Granier
Sophie A
SA
Service de Microbiologie-Hygiène, Hôpital Ambroise Paré AP-HP, Boulogne, France.
Leflon-Guibout
Véronique
V
Goldstein
Fred W
FW
Nicolas-Chanoine
Marie-Hélène
MH
D015373:Bacterial Typing Techniques; D021901:DNA, Intergenic / Q000032:analysis*; D004755:Enterobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D006801:Humans; D007710:Klebsiella Infections / Q000382:microbiology; D041121:Klebsiella oxytoca / Q000145:classification* / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D013045:Species Specificity
D021901:DNA, Intergenic