title: A highly conserved human gene encoding a novel member of WD-repeat family of proteins (WDR13).
abstract: We have identified and characterized a novel member of the WD-repeat motif gene family, WDR13, which contains 9 exons and 8 introns. The gene has been mapped to the genomic locus Xp11.23 by fluorescent in situ hybridization and in silico mapping. Sequence analysis has revealed a continuous open reading frame (ORF) encoding for 485 amino acids with six WD motifs. The expression of this gene has been detected in all the tissues analyzed with significantly varied expression levels among the tissues studied. Analysis of EST clones from various tissues, showing significant homology to WDR13, has identified two spliced variants. The transcription start point has been mapped. Promoter analysis has identified high activity in the 5' UTR, which interestingly showed a testis-specific activity in the transgenic animals studied. The subcellular localization of the WDR13 protein in the nucleus suggests that it may also have a regulatory role in nuclear function along with protein-protein interaction like other members of the WD family of proteins.
authors.affiliation: Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
authors.forename: Bhupendra N
authors.initials: BN
authors.lastname: Singh
authors.forename: Amritha
authors.initials: A
authors.lastname: Suresh
authors.forename: Gogineni
authors.initials: G
authors.lastname: UmaPrasad
authors.forename: Subbaya
authors.initials: S
authors.lastname: Subramanian
authors.forename: Mehar
authors.initials: M
authors.lastname: Sultana
authors.forename: Sandeep
authors.initials: S
authors.lastname: Goel
authors.forename: Satish
authors.initials: S
authors.lastname: Kumar
authors.forename: Lalji
authors.initials: L
authors.lastname: Singh
mesh_terms: D020121:5' Untranslated Regions; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D018797:Cell Cycle Proteins; D002874:Chromosome Mapping; D041321:Chromosomes, Human, X; D017124:Conserved Sequence; D018076:DNA, Complementary; D005091:Exons; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007438:Introns; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016366:Open Reading Frames; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D020121:5' Untranslated Regions; D018797:Cell Cycle Proteins; D018076:DNA, Complementary; D009687:Nuclear Proteins; C479430:WDR13 protein, human