[An experimental study on gene transfection of human interleukin-1 receptor antagonist gene into chondrocytes of temporomandibular joint].
OBJECTIVE
The purpose of this study was to investigate methods and expression of the recombinant human interleukin-1 receptor antagonist gene transfected into chondrocytes of temporomandibular joints (TMJ).


METHODS
Chondrocytes derived from the TMJ of 5-7 months old human fetus were transfected by recombinant human interleukin-1 receptor antagonist gene via cationic liposome (Lipefectine) as a medium. The expressing level of hIL-1ra protein was detected using immunocytochemistry and enzyme-linked immunosorbent assay (ELISA).


RESULTS
The positive results of the immunocytochemistry were found. The positive expression was detected in the cell plasma and the cell culture supernatant, and there was significant difference between the cells with and without gene transfection (P < 0.01).


CONCLUSIONS
This feasible method provides experimented evidence for the future gene therapy of temporomandibular joint osteoarthrosis.
Li
Yisong
Y
Department of Oral and Maxillofacial Surgery, West China College of Stomatology, Sichuan University, Chengdu 610041, China.
Tian
Weidong
W
Wang
Dong
D
D019902:Chondrocytes / Q000166:cytology*; D004797:Enzyme-Linked Immunosorbent Assay; D005333:Fetus; D010714:Phosphatidylethanolamines; D017472:Receptors, Interleukin-1 / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D013704:Temporomandibular Joint / Q000166:cytology*; D014162:Transfection
D010714:Phosphatidylethanolamines; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins; C020888:1,2-dielaidoylphosphatidylethanolamine