[The construction of cDNA expression library from the tentacles of Sagartia rosea].
A cDNA expression library of the tentacles of Sagartia rosea was constructed. The cDNA was cloned into eukaryotical expression plasmid pcDNA3. SMART protocol was used for cDNA library construction and bioinformatics analysis was carried out. 71 novel EST clones were obtained from 130 sequences in the library, of which there were 21 full-length clones, including cytolysin genes, flourescent protein, ubiquinol-cytochrome C reductase gene, elongation factor, ferritin gene riboflavin kinase gene, ribosomal protein. This provides a base for further investigating their biological activity and application.
Liu
Wen-Hua
WH
Open Laboratory for Marine Functional Genomics, National High-Tech Department, Department of Biochemistry, College of Life Science, Zhongshan University, Guangzhou 510275, China.
Wang
Yi-Liang
YL
Chen
Hui-Ping
HP
Jiang
Xiao-Yu
XY
Tu
Hong-Bin
HB
Wei
Jian-Wen
JW
Peng
Wen-Lie
WL
Xu
An-Long
AL
D000818:Animals; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D015723:Gene Library; D012313:RNA / Q000302:isolation & purification; D012615:Sea Anemones / Q000235:genetics*
D018076:DNA, Complementary; D012313:RNA