A novel method to quantify the turnover and release of monocytes from the bone marrow using the thymidine analog 5'-bromo-2'-deoxyuridine.
The present study was designed to develop methods to study the production and release of monocytes from the bone marrow using the thymidine analog 5'-bromo-2'-deoxyuridine (BrdU). Dividing monocytes in bone marrow were labeled with BrdU (MOBrdU), and their release into the blood and disappearance from the circulation were monitored using a double immunostaining method. The first MOBrdU appeared in the circulation 4 h after labeling with BrdU and peaked at 18 h when 34.3 +/- 5.8% of monocytes were labeled. The calculated transit time of monocytes through bone marrow was 38.1 +/- 3.1 h in control rabbits with a half-life (T1/2) of 12.7 h. Instillation of Streptococcus pneumoniae into the lung accelerated the release of monocytes from bone marrow (peak at 10 h) and shortened their bone marrow transit time (27.1 +/- 1.8 vs. 22.6 +/- 0.6, vehicle vs. pneumonia; P < 0.05). We conclude that this nonradioisotope method provides a novel way to monitor monocyte kinetics and confirmed previous reports that a focal pneumonia shortens monocyte marrow transit and increases their release into the circulation.
Goto
Yukinobu
Y
McDonald Research Laboratory, University of British Columbia, St. Paul's Hospital, Vancouver, BC, Canada V6Z1Y6.
Hogg
James C
JC
Suwa
Tatsushi
T
Quinlan
Kevin B
KB
van Eeden
Stephan F
SF
D000818:Animals; D001853:Bone Marrow / Q000502:physiology*; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology; D001973:Bromodeoxyuridine; D002452:Cell Count / Q000295:instrumentation / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology*; D002455:Cell Division / Q000502:physiology*; D005260:Female; D006410:Hematopoiesis / Q000502:physiology*; D007150:Immunohistochemistry / Q000379:methods*; D009000:Monocytes / Q000166:cytology* / Q000502:physiology; D011008:Pneumococcal Infections / Q000473:pathology / Q000503:physiopathology; D011014:Pneumonia / Q000473:pathology / Q000503:physiopathology; D011817:Rabbits; D013234:Stem Cells / Q000166:cytology / Q000502:physiology; D013997:Time Factors
D001973:Bromodeoxyuridine