title: Evaluation of FASTPlaqueTB-RIF for determination of rifampicin resistance in Mycobacterium tuberculosis complex isolates.
abstract: METHODS
  The Microbiology and Clinical Microbiology Department, Gulhane Military Medical Academy, Ankara, Turkey, a tertiary referral hospital in a region endemic for tuberculosis.
  
  
  OBJECTIVE
  To evaluate rifampicin resistance of Mycobacterium tuberculosis complex strains using FASTPlaqueTB-RIF, a rapid and novel bacteriophage-based susceptibility technique.
  
  
  METHODS
  Results of isolates tested with the BACTEC 460 TB system were compared with FASTPlaqueTB-RIF.
  
  
  RESULTS
  Susceptibility to rifampicin of M. tuberculosis complex isolates was tested for 88 isolates using FASTPlaqueTB-RIF. Sixty-seven isolates were susceptible and 21 were resistant to rifampicin using the BACTEC 460 TB system. Overall accuracy for FASTPlaqueTB-RIF was 94.3% (95%CI 87.3-97.5) for the detection of rifampicin susceptibility. The sensitivity and specificity of FASTPlaqueTB-RIF were respectively 100.0% (95%CI 84.5-100) and 92.5% (95%CI 83.6-96.7).
  
  
  CONCLUSIONS
  This study demonstrates that FASTPlaqueTB-RlFM is a rapid and inexpensive test which has a good correlation with the BACTEC 460 TB system.
authors.affiliation: Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy, Ankara, Turkey. okisa@gata.edu.tr
authors.forename: O
authors.initials: O
authors.lastname: Kisa
authors.forename: A
authors.initials: A
authors.lastname: Albay
authors.forename: O
authors.initials: O
authors.lastname: Bedir
authors.forename: O
authors.initials: O
authors.lastname: Baylan
authors.forename: L
authors.initials: L
authors.lastname: Doganci
mesh_terms: D000904:Antibiotics, Antitubercular / Q000494:pharmacology* / Q000627:therapeutic use; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods*; D024881:Drug Resistance, Bacterial; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012293:Rifampin / Q000494:pharmacology* / Q000627:therapeutic use; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000188:drug therapy / Q000382:microbiology*
chemical_list: D000904:Antibiotics, Antitubercular; D012293:Rifampin