Transient association of the sarcoplasmic reticulum Ca2+ ATPase with the Na+/K+-ATPase and H+/K+-ATPase beta-subunits during its biogenesis in Xenopus oocytes.
We examined the effect of the beta-subunits of the Na+/K+ and H+/K+ ATPases on the biogenesis of the sarcoplasmic reticulum (SR) Ca2+ ATPase in Xenopus oocytes. Oocytes were simultaneously injected with cRNAs for both the SR Ca2+ ATPase and the beta-subunit of the Na+/K+ or the H+/K+ ATPase. Immunoprecipitation with antiserum specific for the beta-subunit of the Na+/K+ or the H+/K+ ATPase yielded not only the respective beta-subunit but also the SR Ca2+ ATPase, indicating that the SR Ca2+ ATPase was associated with the beta-subunits of the Na+/K+ and the H+/K+ ATPases. Pulse-chase experiments revealed that the complex between the SR Ca2+ ATPase and the beta-subunit of the Na+/K+ ATPase was formed transiently and dissociated during the course of maturation. This is the first report that demonstrates the association of the SR Ca2+ ATPase with the beta-subunit of the Na+/K+ and H+/K+ ATPases.
Noguchi
Shunsuke
S
Department of Biochemical Engineering and Science, Kyushu Institute of Technology Iizuka, Fukuoka 820-8502, Japan.
Sone
Nobuhito
N
Kawamura
Masaru
M
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D017506:H(+)-K(+)-Exchanging ATPase / Q000378:metabolism*; D018832:Molecular Chaperones / Q000378:metabolism; D009865:Oocytes / Q000378:metabolism*; D010957:Plasmids / Q000378:metabolism; D011233:Precipitin Tests; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D018075:RNA, Complementary / Q000378:metabolism; D012519:Sarcoplasmic Reticulum / Q000201:enzymology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013997:Time Factors; D014357:Trypsin / Q000494:pharmacology; D014981:Xenopus / Q000378:metabolism*; D014982:Xenopus laevis
D018832:Molecular Chaperones; D018075:RNA, Complementary; D014357:Trypsin; D000251:Adenosine Triphosphatases; D017506:H(+)-K(+)-Exchanging ATPase; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase; D002118:Calcium