Cometabolic degradation of chlorinated aromatic compounds.
The degradation of chlorobenzene was investigated with the specially chosen strain Methylocystis sp. GB 14 DSM 12955, using 23 ml headspace vials and in a soil column filled with quaternary aquifer material from a depth of 20 m. A long-term experiment was carried out in this column, situated in a mobile test unit at a contaminated location in Bitterfeld (Germany). Groundwater polluted by chlorobenzene was continuously fed through the column, through which a mixture comprising 4% CH(4) and 96% air was bubbled. Chlorobenzene was oxidized by up to 80% under pure culture conditions in the model experiments and was completely degraded under the mixed culture conditions of the column experiments. Over a period of 4 months, the stability of the biological system was monitored regularly by analyzing the sMMO activity as well as by classical microbiological and molecular biological methods.
Jechorek
M
M
Department of Remediation Research, UFZ Centre for Environmental Research Leipzig-Halle, PO Box 2, Leipzig D-04301, Germany. jechorek@san.ufz.de
Wendlandt
K-D
KD
Beck
M
M
D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques; D002722:Chlorobenzenes / Q000378:metabolism*; D005858:Germany; D006841:Hydrocarbons, Aromatic / Q000378:metabolism; D006843:Hydrocarbons, Chlorinated / Q000378:metabolism; D007220:Industrial Waste / Q000517:prevention & control; D042083:Methylocystaceae / Q000145:classification / Q000378:metabolism*; D012989:Soil Pollutants / Q000378:metabolism; D013696:Temperature; D014874:Water Pollutants, Chemical / Q000378:metabolism*
D002722:Chlorobenzenes; D006841:Hydrocarbons, Aromatic; D006843:Hydrocarbons, Chlorinated; D007220:Industrial Waste; D012989:Soil Pollutants; D014874:Water Pollutants, Chemical; C031294:chlorobenzene