Evaluation of a fluorescence-based PCR method for identification of serogroup a meningococci.
Standard and fluorescence-based PCR assays were developed for the identification of serogroup A meningococci by detection of the mynA gene. This assay was evaluated using bacterial cultures but provides the sensitivity required for the detection of the mynA gene from bodily fluids during meningococcal disease.
Diggle
M A
MA
Scottish Meningococcus and Pneumococcus Reference Laboratory, Stobhill Hospital, Glasgow, United Kingdom.
Smith
K
K
Girvan
E K
EK
Clarke
S C
SC
D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D001826:Body Fluids / Q000382:microbiology; D003470:Culture Media; D005456:Fluorescent Dyes; D006801:Humans; D008589:Meningococcal Infections / Q000382:microbiology; D008969:Molecular Sequence Data; D038381:Neisseria meningitidis, Serogroup A / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D001426:Bacterial Proteins; D003470:Culture Media; D005456:Fluorescent Dyes