Metabolism of benzyl alcohol via catechol ortho-pathway in methylnaphthalene-degrading Pseudomonas putida CSV86.
Pseudomonas putida CSV86 metabolizes 1- and 2-methylnaphthalene through distinct catabolic and detoxification pathways. In spite of the similarity in the steps involved in the methylnaphthalene detoxification and the toluene side-chain hydroxylation pathways, the strain failed to utilize toluene or xylenes. However, it could grow on benzyl alcohol, 2- and 4-hydroxybenzyl alcohol. Metabolic studies suggest that the benzyl alcohol metabolism proceeds via the benzaldehyde, benzoate, and catechol ortho-cleavage pathway, in contrast to the well established catechol meta-cleavage pathway. Carbon source-dependent enzyme activity studies suggest that the degradation of aromatic alcohol involves two regulons. Aromatic alcohol induces the upper regulon, which codes for aromatic alcohol- and aromatic aldehyde-dehydrogenase and converts alcohol into acid. The aromatic acid so generated induces the specific lower regulon and is metabolized via either the ortho- or the meta-cleavage pathway. CSV86 cells transform 1- and 2-methylnaphthalene to 1- and 2-hydroxymethyl naphthalene, which are further converted to the respective naphthoic acids due to the basal level expression and broad substrate specificity of the upper regulon enzymes.
Basu
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Biotechnology Group, School of Biosciences and Bioengineering, Indian Institute of Technology, 400 076 Bombay, Mumbai, India.
Dixit
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Phale
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D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000445:Aldehyde Oxidoreductases / Q000378:metabolism; D001547:Benzaldehydes / Q000378:metabolism; D019817:Benzoic Acid / Q000378:metabolism; D019905:Benzyl Alcohol / Q000378:metabolism*; D001592:Benzyl Alcohols / Q000378:metabolism; D001673:Biodegradation, Environmental; D015964:Gene Expression Regulation, Bacterial; D009281:Naphthalenes / Q000378:metabolism; D016958:Pseudomonas putida / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D018085:Regulon / Q000235:genetics; D013379:Substrate Specificity
D001547:Benzaldehydes; D001592:Benzyl Alcohols; D009281:Naphthalenes; C018966:4-hydroxybenzyl alcohol; D019817:Benzoic Acid; C025968:1-methylnaphthalene; D000429:Alcohol Oxidoreductases; C055882:aryl-alcohol dehydrogenase; D000445:Aldehyde Oxidoreductases; D019905:Benzyl Alcohol; C027384:2-methylnaphthalene; C032175:benzaldehyde