[Effect of 1,25-dihydroxyvitamin D3 and 17beta-estradiol on hpl-bp74 gene regulation in breast adenocarcinoma cell line].
Data are provided on the upregulation of heterochromatin protein binding protein 74 gene (hpl-bp74) at RNA transcription level under the influence of stimulation of 1,25-dihydroxyvitamin D3. We also showed the absence of action of 17 beta-estradiol on the transcription level of hpl-bp74 breast adencarcinoma cell line (MCF7). The protein encoded by gene hpl-bp74 can be involved in generalized transcriptional regulation and thus take part in inhibition of proliferation by 1,25(OH)2D3. Computer analyses of DNA sequences of hpl-bp74 enabled us to identify some potential hormone responsive elements responsible for the binding of nuclear receptor for vitamin D with the DNA motifs.
Aksenov
N L
NL
Institute of Cytology RAS, 194064, St. Petersburg, Russia. NiLAksenov@mail.ru
Mikhel'son
V M
VM
Polozhintsev
B I
BI
Spivak
I M
IM
Tuohima
P
P
Ylikomi
T
T
D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002117:Calcitriol / Q000494:pharmacology*; D000090266:Chromobox Protein Homolog 5; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics* / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D012333:RNA, Messenger / Q000378:metabolism; D018167:Receptors, Calcitriol / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured; D014809:Vitamin D-Binding Protein / Q000378:metabolism
D002868:Chromosomal Proteins, Non-Histone; D012333:RNA, Messenger; D018167:Receptors, Calcitriol; D014157:Transcription Factors; D014809:Vitamin D-Binding Protein; D000090266:Chromobox Protein Homolog 5; D004958:Estradiol; D002117:Calcitriol