title: [Preparation and characterization of monoclonal antibody against human telomeric repeat binding factor 1].
abstract: OBJECTIVE
  To prepare a monoclonal antibody against human telomeric repeat binding factor 1 (TRF1) protein and explore its biological characteristics.
  
  
  METHODS
  BALB/c mice were immunized with GST-TRF1(33-277) fusion protein for the preparation of monoclonal antibody by hybridoma technique. The obtained antibody was used for clinical assay by Western-blot and immunohistochemical staining.
  
  
  RESULTS
  One strain of hybridoma was obtained. It was confirmed by Western-blot that the antibody specifically recognized the 60 kD TRF1 protein. Immunohistochemical staining of the antibody showed that TRF1 protein located in the cytoplasm of epithelial cells and bone marrow cells.
  
  
  CONCLUSIONS
  A TRF1 monoclonal antibody, with high specificity was developed. It is useful for detection of TRF1 protein in tissue specimens.
authors.affiliation: The First Affiliated Hospital, Medical College of Zhejiang University, Hangzhou 310003, China.
authors.forename: He
authors.initials: H
authors.lastname: Huang
authors.forename: Jimin
authors.initials: J
authors.lastname: Shi
authors.forename: Qiaofang
authors.initials: Q
authors.lastname: Chen
authors.forename: Yi
authors.initials: Y
authors.lastname: Luo
authors.forename: Wei
authors.initials: W
authors.lastname: Ding
authors.forename: Jiyu
authors.initials: J
authors.lastname: Lou
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015153:Blotting, Western; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D011993:Recombinant Fusion Proteins / Q000276:immunology; D035321:Telomeric Repeat Binding Protein 1 / Q000032:analysis / Q000235:genetics / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D011993:Recombinant Fusion Proteins; D035321:Telomeric Repeat Binding Protein 1