Common characteristics of the human and rhesus macaque CD1e molecules: conservation of biochemical and biological properties during primate evolution.
In humans, a family of five genes encodes the CD1 molecules. Four of these proteins, CD1a, b, c, and d, are expressed on the plasma membrane and traffic between the cell surface and endocytic compartments, where they are loaded with antigenic glycolipids. The existence of human CD1e was demonstrated recently. This molecule surprisingly remains inside the cell, accumulating mainly in the Golgi compartments of immature dendritic cells and in the late endosomes of mature dendritic cells. In the latter compartments, CD1e is cleaved and becomes soluble. To determine whether these properties were specific to human CD1e, we investigated the presence and characteristics of CD1e in the rhesus macaque, an evolutionarily distant species of the primate lineage. Our results show that the cellular and biochemical properties of the human and simian CD1e molecules are similar, suggesting that the particular intracellular distribution of CD1e is important for its physiological and/or immunological function.
Angénieux
Catherine
C
INSERM EP 99-08, Etablissement Français du Sang-Alsace, Strasbourg, France.
Salamero
Jean
J
Fricker
Dominique
D
Wurtz
Jean-Marie
JM
Maître
Blandine
B
Cazenave
Jean-Pierre
JP
Hanau
Daniel
D
de la Salle
Henri
H
D000595:Amino Acid Sequence; D000818:Animals; D018949:Antigens, CD1 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D003713:Dendritic Cells / Q000276:immunology; D019143:Evolution, Molecular; D006801:Humans; D008253:Macaca mulatta / Q000235:genetics* / Q000276:immunology*; D008969:Molecular Sequence Data; D009000:Monocytes / Q000276:immunology; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014162:Transfection
D018949:Antigens, CD1; C417738:CD1e antigen; D018076:DNA, Complementary; D011994:Recombinant Proteins