Antiinflammatory activity of alpha-hederin methyl ester from the alkaline hydrolysate of the butanol fraction of Kalopanax pictus bark extract.
Three antiinflammatory saponin components were isolated from the alkaline hydrolysate of a butanol-soluble portion of Kalopanax pictus bark extract through an in vivo activity-guided fractionation procedure. The hydrolysate showed inhibition of adjuvant induced arthritis in rats. After further fractionation, the ethyl acetate fraction exhibited antiarthritic activity, which resulted in the isolation of alpha-hederin, alpha-hederin methyl ester, and kalopanaxsaponin I. All compounds showed inhibition of vascular permeability in mice, but only alpha-hederin methyl ester showed anticarrageenan activity in rats and antiarthritic activity in rats and mice.
Li
Da Wei
DW
Natural Products Research Institute, Seoul National University, Korea.
Hyun
Jin Ee
JE
Jeong
Choon Sik
CS
Kim
Yeong Shik
YS
Lee
Eun Bang
EB
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000302:isolation & purification* / Q000627:therapeutic use; D001169:Arthritis, Experimental / Q000139:chemically induced / Q000188:drug therapy; D000440:Butanols / Q000737:chemistry / Q000302:isolation & purification* / Q000627:therapeutic use; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D004952:Esters; D006868:Hydrolysis; D036882:Kalopanax; D008297:Male; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification* / Q000627:therapeutic use; D024301:Plant Bark; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000627:therapeutic use
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000440:Butanols; D004952:Esters; D010936:Plant Extracts; D012503:Saponins; C010177:beta-hederin; D009828:Oleanolic Acid