title: A search for candidate genes for lipodystrophy, obesity and diabetes via gene expression analysis of A-ZIP/F-1 mice.
abstract: Genome scans for diabetes have identified many regions of the human genome that correlate with the disease state. To identify candidate genes for type 2 diabetes, we examined the transgenic A-ZIP/F-1 mouse. This mouse model has no white fat, resulting in abnormal levels of glucose, insulin, and leptin, making the A-ZIP/F-1 mice a good model for lipodystrophy and insulin resistance. We used cDNA-based microarrays to find differentially expressed genes in four tissues of these mice. We examined these results in the context of human linkage scans for lipodystrophy, obesity, and type 2 diabetes. We combined 199 known human orthologs of the misregulated mouse genes with 33 published human genome scans on a genome map. Integrating expression data with human linkage results permitted us to suggest and prioritize candidate genes for lipodystrophy and related disorders. These genes include a cluster of 3 S100A genes on chromosome 1 and SLPI1 on chromosome 20.
authors.affiliation: Laboratory of Metabolism, National Institutes of Health, Baltimore, MD 21224, USA.
authors.forename: Alain A
authors.initials: AA
authors.lastname: Mir
authors.forename: Maxim V
authors.initials: MV
authors.lastname: Myakishev
authors.forename: Oksana O
authors.initials: OO
authors.lastname: Polesskaya
authors.forename: Jaideep
authors.initials: J
authors.lastname: Moitra
authors.forename: David
authors.initials: D
authors.lastname: Petersen
authors.forename: Lance
authors.initials: L
authors.lastname: Miller
authors.forename: Andras
authors.initials: A
authors.lastname: Orosz
authors.forename: Charles
authors.initials: C
authors.lastname: Vinson
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics; D002890:Chromosomes, Human, Pair 20 / Q000235:genetics; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D023281:Genomics; D006801:Humans; D008060:Lipodystrophy / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D009765:Obesity / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis