FP-TDI SNP scoring by manual and statistical procedures: a study of error rates and types.
For technologies that are commonly used in ordinary laboratories such as fluorescence-polarization detection with template-directed, dye-terminator incorporation (FP-TDI), SNP genotype scoring is usually done manually. Here we study rates of errors and missing genotypes obtained with this procedure. We also introduce three statistical genotype scoring methods to examine whether they form a viable alternative. Data consisted of eight SNPs typed in about 1400 individuals from 268 pedigrees. The statistical procedures performed better on several internal criteria, such as the number of Mendelian errors, and showed much higher agreement with discrepant genotypes re-scored by two raters. The best results were obtained with the statistical procedure that incorporated information about regularities in the error structure of the FP-TDI data. We estimated that there were about 1.6% more errors if genotypes were scored manually. About 0.6% of these errors could be explained by data manipulation errors, leaving 1% as the result of possible incorrect scoring. There were 3.3% more missing genotypes in the manual scoring due to errors in data manipulation (1.7%) and conservative scoring (1.6%).
van den Oord
E J C G
EJ
Department of Psychiatry, Virginia Commonwealth University, P.O. Box 980126, Richmond, VA 23298-0126, USA. ejvandenoord@vcu.edu
Jiang
Y
Y
Riley
B P
BP
Kendler
K S
KS
Chen
X
X
D000465:Algorithms; D004252:DNA Mutational Analysis / Q000379:methods*; D005454:Fluorescence Polarization / Q000379:methods; D020869:Gene Expression Profiling / Q000379:methods*; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008957:Models, Genetic; D015233:Models, Statistical; D010375:Pedigree; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011786:Quality Control; D015203:Reproducibility of Results; D012559:Schizophrenia / Q000235:genetics*; D012680:Sensitivity and Specificity; D016037:Single-Blind Method