title: The apoptosis-associated protein BNIPL interacts with two cell proliferation-related proteins, MIF and GFER.
abstract: Bcl-2/adenovirus E1B 19 kDa interacting protein 2-like, BNIP-2-like (BNIPL) is a recently cloned and characterized apoptosis-associated protein that shares 72% homology with BNIP-2. It is highly expressed in human placenta and lung. A yeast two-hybrid system was used to obtain two BNIPL-interacting proteins, MIF (macrophage migration inhibitory factor) and GFER (growth factor erv1 (Saccharomyces cerevisiae)-like). The interactions were confirmed by glutathione S-transferase pull-down assay in vitro and co-immunoprecipitation assay in vivo. Colony formation assay and cell proliferation test suggest that overexpression of BNIPL could inhibit the growth of BEL-7402 cells. These findings suggest that BNIPL may physically bind to cell proliferation-related proteins, MIF and GFER.
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Shen
authors.forename: Jian
authors.initials: J
authors.lastname: Hu
authors.forename: Hong
authors.initials: H
authors.lastname: Lu
authors.forename: Ming
authors.initials: M
authors.lastname: Wu
authors.forename: Wenxin
authors.initials: W
authors.lastname: Qin
authors.forename: Dafang
authors.initials: D
authors.lastname: Wan
authors.forename: Yu-Yang
authors.initials: YY
authors.lastname: Li
authors.forename: Jianren
authors.initials: J
authors.lastname: Gu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000378:metabolism*; D006801:Humans; D011233:Precipitin Tests; D011485:Protein Binding; D020798:Two-Hybrid System Techniques
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C450834:BNIPL protein, human; D002352:Carrier Proteins; D018797:Cell Cycle Proteins