title: Investigations on the binding of mercury ions to albumins employing differential pulse voltammetry.
abstract: Binding of mercury to BSA and Ovalbumin was investigated by Differential Pulse Voltammetry. The method relies on the direct monitoring of peak current variation due to mercury oxidation in the presence of these two albumins. Linear calibration graphs were obtained for both BSA and Ovalbumin in concentrations ranging from 2.49 x 10(-9) to 19.6 x 10(-9) mol L(-1). The acquired data was used to quantify these two proteins independently and to calculate the dissociation constants of Hg-BSA and Hg-Ovalbumin complexes.
authors.affiliation: Laboratório de Espectrometria de Massa, Embrapa Recursos Genéticos e Biotecnologia, P O Box 02372, 70.770-900, Brasília-DF, BRAZIL.
authors.forename: Clarissa Silva Pires de
authors.initials: CS
authors.lastname: Castro
authors.forename: Jurandir Rodrigues
authors.initials: JR
authors.lastname: SouzaDe
authors.forename: Carlos
authors.initials: C
authors.lastname: Bloch
mesh_terms: D000418:Albumins / Q000032:analysis / Q000378:metabolism*; D007700:Kinetics; D008628:Mercury / Q000378:metabolism*
chemical_list: D000418:Albumins; D008628:Mercury