title: High anti-Helicobacter pylori antibody seropositivity associated with the combination of IL-8-251TT and IL-10-819TT genotypes.
abstract: BACKGROUND
  Helicobacter pylori induces inflammation of gastric mucosa regulated by several interleukins. This study examined associations between anti-Helicobacter pylori immunoglobulin G antibody seropositivity and functional polymorphisms of interleukin-8 T-251 A and interleukin-10 T-819C.
  
  
  METHODS
  The subjects were 454 health check-up examinees (126 males and 328 females) without a history of cancer, aged 35-85 years, residing in Nagoya, Japan. After written informed consent was obtained individually, residual blood was anonymously applied for anti-Helicobacter pylori immunoglobulin G antibody testing and genotyping by the polymerase chain reaction with confronting two-pair primers.
  
  
  RESULTS
  The genotype frequency of interleukin-8 T-251 A was 52.2% for TT, 39.5% for TA, and 8.3% for AA, and that of interleukin-10 T-819C was 49.5% for TT, 39.9% for TC and 10.6% for CC. Although the differences in the positive rates among the genotypes were not marked, 115 individuals with interleukin-8-251TT (low expression genotype) and interleukin-10-819TT (high expression genotype) had a higher rate (63.5%) than the others (52.0%). Relative to the combination of interleukin-8-251TT and interleukin-10-819TT, the sex-age-adjusted odds ratio for those with the other combinations was 0.62 (95% confidence interval, 0.39-0.98). The adjusted odds ratio among 65 current smokers was 0.13 (0.03-0.61).
  
  
  CONCLUSIONS
  The observed association suggests that individuals with interleukin-8-251TT and interleukin-10-819TT, a combination presumably causing mild inflammation, have a higher probability of the continuing Helicobacter pylori infection, especially among current smokers.
authors.affiliation: Division of Epidemiology and Prevention, Aichi Cancer Center Research Center, Nagoya, Japan.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Hamajima
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Katsuda
authors.forename: Keitaro
authors.initials: K
authors.lastname: Matsuo
authors.forename: Toshiko
authors.initials: T
authors.lastname: Saito
authors.forename: Kaoru
authors.initials: K
authors.lastname: Hirose
authors.forename: Manami
authors.initials: M
authors.lastname: Inoue
authors.forename: Toshiro Take
authors.initials: TT
authors.lastname: Zaki
authors.forename: Kazuo
authors.initials: K
authors.lastname: Tajima
authors.forename: Suketami
authors.initials: S
authors.lastname: Tominaga
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000097:blood; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D016481:Helicobacter Infections / Q000235:genetics* / Q000276:immunology*; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D016209:Interleukin-8 / Q000235:genetics*; D008297:Male; D008875:Middle Aged
chemical_list: D000907:Antibodies, Bacterial; D016209:Interleukin-8; D016753:Interleukin-10