Molecular characterization of NbPAF encoding the alpha6 subunit of the 20S proteasome in Nicotiana benthamiana.
The 26S proteasome involved in degradation of proteins covalently modified with polyubiquitin consists of the 20S proteasome and 19S regulatory complex. The NbPAF gene encoding the alpha6 subunit of the 20S proteasome was identified from Nicotiana benthamiana. NbPAF exhibits high sequence homology with the corresponding genes from Arabidopsis, human and yeast. The deduced amino acid sequence of NbPAF reveals that this protein contains the proteasome alpha-type subunits signature and nuclear localization signal at the N-terminus. The genomic Southern blot analysis suggests that the N. benthamiana genome contains one copy of NbPAF. The NbPAF mRNA was detected abundantly in flowers and weakly in roots and stems, but it was almost undetectable in mature leaves. In response to stresses, accumulation of the NbPAF mRNA was stimulated by methyl jasmonate, NaCl and salicylic acid, but not by abscisic acid and cold treatment in leaves. The NbPAF-GFP fusion protein was localized in the cytoplasm and nucleus.
Kim
Moonil
M
Laboratory of Plant Genomics, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-600, Korea.
Yang
Kyoung-Sil
KS
Kim
Yu-Kyung
YK
Paek
Kyung-Hee
KH
Pai
Hyun-Sook
HS
D000040:Abscisic Acid / Q000494:pharmacology; D000085:Acetates / Q000494:pharmacology; D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins / Q000737:chemistry; D019470:Biolistics; D002467:Cell Nucleus / Q000201:enzymology; D003080:Cold Temperature; D003517:Cyclopentanes / Q000494:pharmacology; D003546:Cysteine Endopeptidases / Q000096:biosynthesis / Q000235:genetics*; D003593:Cytoplasm / Q000201:enzymology; D018744:DNA, Plant / Q000235:genetics; D004790:Enzyme Induction / Q000187:drug effects; D017343:Genes, Plant; D006801:Humans; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000096:biosynthesis / Q000235:genetics*; D019697:Onions / Q000166:cytology; D009928:Organ Specificity; D054883:Oxylipins; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D018514:Plant Structures / Q000201:enzymology; D046988:Proteasome Endopeptidase Complex; D021122:Protein Subunits / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry; D020156:Salicylic Acid / Q000494:pharmacology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride / Q000494:pharmacology; D013045:Species Specificity; D014026:Nicotiana / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*
D000085:Acetates; D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D018744:DNA, Plant; D009097:Multienzyme Complexes; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D021122:Protein Subunits; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D012965:Sodium Chloride; D000040:Abscisic Acid; C072239:methyl jasmonate; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex; D020156:Salicylic Acid