[An experimental study on circadian rhythm of the proliferative index of mandibular osteoblast in goats].
OBJECTIVE
The aim of this study was to investigate the circadian rhythm of the proliferative index of mandibular osteoblasts of goats in a normal situation.


METHODS
Twenty four healthy goats were selected and randomly divided into six groups. They were killed, and a piece of bone tissue with 2 cm x 2 cm x 0.5 cm was obtained from right mandibular mental foramen region at 0:00, 4:00, 8:00, 12:00, 16:00 and 20:00 time points, after they had been fed for one week. The primary cell culture method was used to cultivate osteoblasts from the bone. The method of anti-5-bromodeoxyuridine monoclonal antibody and the flow cytometric analysis were used to detect the proliferative index of the mandibular osteoblasts at different time. The data were analyzed using the method of single cosine rhythmometry.


RESULTS
The proliferative indexes of the mandibular osteoblasts of goats were higher at the nighttime and lower in the daytime. The undulation of the proliferative indexes displayed a significant circadian rhythm (P < 0.01). The peak value was obtained at about 19:44, and the lowest value at about 7:44.


CONCLUSIONS
The proliferative indexes of the mandibular osteoblasts of goats are not stable within 24 hours in a normal situation, but with significant circadian rhythm. There are different proliferative indexes at different time points.
Zheng
Xiaohui
X
Department of Oral and Maxillofacial Surgery, West China College of Stomatology, Sichuan University, Chengdu 610041, China.
Tian
Weidong
W
Long
Jie
J
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D002940:Circadian Rhythm; D005434:Flow Cytometry; D006041:Goats; D008334:Mandible / Q000166:cytology*; D010006:Osteoblasts / Q000166:cytology*; D011897:Random Allocation