In vitro inhibitory effect of EGTA on macrophage adhesion: endodontic implications.
Ethylene glycol-bis-(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) is an specific calcium ion chelator proposed as endodontic irrigant. This study investigates the effect of EGTA on substrate adherence capacity of rat inflammatory macrophages. Inflammatory macrophages were obtained from Wistar rats and resuspended in RPMI-1640 medium. Substrate adherence capacity assays were carried out in Eppendorf tubes for 15 min of incubation at 37 degrees C in a humidified atmosphere of 5% CO2. The adherence index was calculated. Results showed that EGTA decreased substrate adherence capacity of inflammatory macrophages in a time- and dose-dependent manner. The EGTA concentration that caused half-maximal inhibition (IC50) was 202 +/- 32 mM (p < 0.01). EDTA was more potent than EGTA in inhibiting macrophage adherence (IC50 = 185 +/- 22 mM). Calcium chloride (10 mM) decreased the EGTA inhibitory effect on adherence index by 60.2% (p < 0.01). We conclude that EGTA significantly decreased substrate adherence capacity of macrophages.
Segura-Egea
Juan J
JJ
Department of Stomatology, School of Dentistry, University of Seville, Spain.
Jiménez-Rubio
Alicia
A
Rios-Santos
José V
JV
Velasco-Ortega
Eugenio
E
Calvo-Gutierrez
Juan R
JR
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002122:Calcium Chloride / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D002614:Chelating Agents / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004492:Edetic Acid / Q000008:administration & dosage / Q000494:pharmacology; D004533:Egtazic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D006813:Humidity; D008264:Macrophages / Q000187:drug effects*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012388:Root Canal Irrigants / Q000494:pharmacology*; D013223:Statistics as Topic; D013696:Temperature
D002614:Chelating Agents; D012388:Root Canal Irrigants; D004533:Egtazic Acid; D004492:Edetic Acid; D002122:Calcium Chloride; D002118:Calcium