Study of the reaction of proteins with 3-hydroxy-4-(2-hydroxy-4-sulfo-1-naphthalenyl)azo (Cal-Red) by Rayleigh light scattering technique.
This is the first report on the determination of proteins with 3-hydroxy-4-(2-hydroxy-4-sulfo-1-naphthalenyl)azo (Cal-Red) by Rayleigh light scattering (RLS). At pH 4.07, the weak RLS of Cal-Red can be enhanced greatly by the addition of proteins. On this basis, the reaction of Cal-Red and proteins was studied. A new quantitative determination method for proteins has been developed. This method is very sensitive (0.45-36.9 microgml(-1) for bovine serum albumen (BSA)), rapid (<2min), simple (one step), and tolerant of most interfering substances. The maximum binding number of Cal-Red to BSA was 143 and the binding constant was 4.1x10(6)mol(-1)L. Four samples of total protein in human serum were determined and the maximum relative error is no more than 3%.
Dong
Lijun
L
Department of Chemistry, Lanzhou University, People's Republic of China.
He
Jiang
J
Li
Qianfeng
Q
Chen
Xingguo
X
Hu
Zhide
Z
D000596:Amino Acids / Q000737:chemistry; D001391:Azo Compounds / Q000737:chemistry*; D001665:Binding Sites; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008027:Light; D008670:Metals / Q000737:chemistry; D008956:Models, Chemical; D009281:Naphthalenes / Q000737:chemistry*; D010777:Photochemistry / Q000379:methods*; D011506:Proteins / Q000032:analysis*; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000032:analysis; D012965:Sodium Chloride / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry
C479961:3-hydroxy-4-(2-hydroxy-4-sulfo-1-naphthalenyl)azo; D000596:Amino Acids; D001391:Azo Compounds; D008670:Metals; D009281:Naphthalenes; D011506:Proteins; D013501:Surface-Active Agents; D012710:Serum Albumin, Bovine; D012965:Sodium Chloride