[Effect of B7-2 expression plasmid on the specific immune response induced by HBV DNA vaccine].
OBJECTIVE
To determine whether B7-2 could improve hepatitis B virus (HBV)-specific immunity induced by inoculation of a plasmid encoding HBV preS2+S HBV DNA vaccine.


METHODS
B7-2 expression plasmids were inoculated with the DNA vaccine in the gastrocemius muscles. Then the cytolytic T Lymphocyte activity (CTL), the delayed-type hypersensitivity (DTH) response and the titers of anti-HBs were analysed.


RESULTS
The DTH response and CTL activity were significantly enhanced when B7-2 expression plasmids were coinoculated with the DNA vaccine (P<0.01), but the titers of anti-HBs were unaffected (P>0.05).


CONCLUSIONS
The results suggest that B7-2 expression plasmid could improve HBV specific cell-mediated immunity induced by HBV DNA vaccine, and the gene-based coinoculation strategy using HBV viral antigen and B7-2 costimmulating could be a powerful means of combating HBV infection.
Wang
Yanjun
Y
Central Laboratory, Shandong Provincial Hospital, Jinan 250021, China.
Jin
Qinghan
Q
Song
Changwen
C
Sun
Bin
B
D000276:Adjuvants, Immunologic; D000818:Animals; D015703:Antigens, CD / Q000276:immunology*; D051940:B7-2 Antigen; D006510:Hepatitis B Antibodies / Q000096:biosynthesis; D017325:Hepatitis B Vaccines / Q000276:immunology*; D006968:Hypersensitivity, Delayed / Q000276:immunology; D008297:Male; D008562:Membrane Glycoproteins / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010957:Plasmids; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology*
D000276:Adjuvants, Immunologic; D015703:Antigens, CD; D051940:B7-2 Antigen; C497965:Cd86 protein, mouse; D006510:Hepatitis B Antibodies; D017325:Hepatitis B Vaccines; D008562:Membrane Glycoproteins; D019444:Vaccines, DNA