Earrings and padlocks for the double helix: topological labeling of duplex DNA.
Concatenation of hybridization probe with DNA target is crucial for highly localized detection of targeted sequences and might also be used in various gene-therapy applications. Several approaches based on the attachment of a circular oligonucleotide to designated DNA sites have been proposed. Recently, earring-like probes provide a true topological linkage between a probe and the target, thus allowing the DNA labeling by essentially immobile tags. The latest development in this direction takes advantage of oligonucleotide uptake by supercoiled DNA and is an important step forward.
Demidov
Vadim V
VV
Center for Advanced Biotechnology, Boston University, 36 Cummington Street, Boston, MA 02215, USA. vvd@bu.edu
D004247:DNA / Q000737:chemistry*; D015342:DNA Probes / Q000138:chemical synthesis* / Q000737:chemistry; D004270:DNA, Circular / Q000737:chemistry; D021141:Nucleic Acid Amplification Techniques; D009690:Nucleic Acid Conformation; D017422:Sequence Analysis, DNA / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods*
D015342:DNA Probes; D004270:DNA, Circular; D004247:DNA