Heat shock response by the hyperthermophilic archaeon Pyrococcus furiosus.
Collective transcriptional analysis of heat shock response in the hyperthermophilic archaeon Pyrococcus furiosus was examined by using a targeted cDNA microarray in conjunction with Northern analyses. Differential gene expression suggests that P. furiosus relies on a cooperative strategy of rescue (thermosome [Hsp60], small heat shock protein [Hsp20], and two VAT-related chaperones), proteolysis (proteasome), and stabilization (compatible solute formation) to cope with polypeptide processing during thermal stress.
Shockley
Keith R
KR
Department of Chemical Engineering, North Carolina State University, Raleigh 27695-7905, USA.
Ward
Donald E
DE
Chhabra
Swapnil R
SR
Conners
Shannon B
SB
Montero
Clemente I
CI
Kelly
Robert M
RM
D019843:Archaeal Proteins / Q000235:genetics / Q000378:metabolism*; D015152:Blotting, Northern; D020869:Gene Expression Profiling; D019848:Gene Expression Regulation, Archaeal; D018869:Heat-Shock Response; D020411:Oligonucleotide Array Sequence Analysis; D017510:Protein Folding; D020027:Pyrococcus furiosus / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
D019843:Archaeal Proteins