title: Identification and quantification of antigens associated with HLA class I molecules on a bladder tumour cell line. Implications for vaccine design.
abstract: The aim of this study was to isolate and identify antigenic peptides associated with HLA class I molecules on a bladder tumour cell line. HLA-A1 molecules were purified using an immunobead-purification technique and following elution of nonapeptides associated with the complex, their HPLC profile was determined by Tandem mass spectrometry (MS/MS). Three peptides were identified namely: (1) P991 (VTDPGNLLY); (2) P1041.5 (LTDLGFLVY), and (3) P1057.7 (LTDPHLLSY); these sequences matched elements of hepatitis B, MAGE1-A1 and herpes simplex viruses. These antigens had half-lives of approximately 120 min which is within the theoretical range of such short peptides. These results indicate that identification of MHC-associated peptides is possible without using an assay for cytotoxic T cells. Although this approach was applied on a relatively small scale, broader applications can be foreseen.
authors.affiliation: Uro/Oncology Research and Department of Immunology, St. Bartholomew's and The Royal London School of Medicine and Dentistry, London EC1A 7BE, UK.
authors.forename: Nasir
authors.initials: N
authors.lastname: Torabi-Pour
authors.forename: W John W
authors.initials: WJ
authors.lastname: Morrow
authors.forename: Roghieh
authors.initials: R
authors.lastname: Saffie
authors.forename: Prashant G
authors.initials: PG
authors.lastname: Gowda
authors.forename: D
authors.initials: D
authors.lastname: Perrett
authors.forename: Timothy R D
authors.initials: TR
authors.lastname: Oliver
mesh_terms: D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D015788:HLA-A1 Antigen / Q000378:metabolism*; D006801:Humans; D013058:Mass Spectrometry; D009842:Oligopeptides / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000276:immunology*
chemical_list: D015788:HLA-A1 Antigen; D009842:Oligopeptides; D010446:Peptide Fragments