title: Variants of human papillomavirus types 53, 58 and 66 identified in Central Brazil.
abstract: The present study on molecular characterization of human papillomaviruses occurring in Central Brazil, describes two variants each of HPV-53 and HPV-58 and one variant of HPV-66 detected in samples from smears of women showing cervical intraepithelial neoplasia grade II (CIN II). Samples were assayed by PCR using MY09/ MY11 consensus primers, followed by restriction fragment length polymorphism typing. The five isolates showed atypical restriction fragment length profile and MY09/MY11 L1 PCR products were subsequently sequenced. Isolate Bsb-02 and Bsb-08 showed, respectively, 99% similarity to HPV-58 IS404 and 100% to HPV-58 IS417 previously described in the African Continent. Isolates Bsb-61 and Bsb-63 showed 98% similarity to HPV-53, and isolate Bsb-68, 97% similarity to HPV-66. Amino acid substitutions were found in two samples: one in Bsb-02 (T to N) at position 375 and the other in Bsb-61 (S to C) at position 343. Although all the substitutions in Bsb-68 proved to be silent, this sample showed the highest value of pairwise evolutionary distance (2.05%). In countries such as Brazil, where the virus prevalence is high and ethnicity, as well as socio-demographic characteristics, vary according to different regions, HPV variability must be wider and not yet clearly defined.
authors.affiliation: Departamento de Biologia Celular, Instituto de Biologia, Universidade de Brasília, DF, Brazil.
authors.forename: Daniela Marreco
authors.initials: DM
authors.lastname: Cerqueira
authors.forename: Geni Noceti de Lima
authors.initials: GN
authors.lastname: Camara
authors.forename: Márcio Rojas
authors.initials: MR
authors.lastname: da Cruz
authors.forename: Evandro Oliveira
authors.initials: EO
authors.lastname: Silva
authors.forename: Marcelo de Macelo
authors.initials: Mde M
authors.lastname: Brígido
authors.forename: Luciano Gonçalves de Souza
authors.initials: LG
authors.lastname: Carvalho
authors.forename: Cláudia Renata Fernandes
authors.initials: CR
authors.lastname: Martins
mesh_terms: D019943:Amino Acid Substitution; D001938:Brazil / Q000453:epidemiology; D005260:Female; D018703:Founder Effect; D014644:Genetic Variation; D006801:Humans; D008302:Mali / Q000208:ethnology; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000453:epidemiology / Q000821:virology*; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012689:Sequence Homology, Nucleic Acid; D014412:Tumor Virus Infections / Q000453:epidemiology / Q000821:virology*; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology / Q000821:virology*; D002578:Uterine Cervical Dysplasia / Q000453:epidemiology / Q000821:virology*