title: Oxidative stress and insulin requirements in patients with recent-onset type 1 diabetes.
abstract: The purpose of this study was to analyze biochemical measures of oxidative stress and assess their relationship to insulin requirements early in type 1 diabetes. Thirty-seven patients enrolled in a 3-yr longitudinal study of the effects of oxidative stress on the early natural history of this disorder. We measured plasma nitrite and nitrate (collectively NOx), nitrotyrosine, and 8-iso-prostaglandin F(2alpha) (8-iso-PGF(2alpha)). Plasma NOx was 34.0 +/- 4.9 micro mol/liter in the control subjects and 52.4 +/- 5.1, 50.0 +/- 5.1, and 49.0 +/- 5.2 micro mol/liter in the diabetic patients at the first, second, and third evaluations, respectively (P < 0.01). Nitrotyrosine was 13.3 +/- 2.0 micro mol/liter in controls and 26.8 +/- 4.4, 26.1 +/- 4.3, and 32.7 +/- 4.3 micro mol/liter in the diabetic patients (P < 0.01). 8-Iso-PGF(2alpha) was higher in the poorly controlled than in the well controlled patients. NOx correlated with insulin dose at the first (P < 0.05), second (P < 0.025), and third (P < 0.05) evaluations. 8-Iso-PGF(2alpha) correlated with insulin dose at the first (P < 0.01) and third (P < 0.0025) evaluations. Systemic measures of oxidative stress correlate with insulin requirements in early type 1 diabetes. These results suggest that oxidative stress is taking place in the pancreas and damaging the beta-cell.
authors.affiliation: Department of Medicine, West Virginia University, School of Medicine, Robert C. Byrd Health Sciences Center, Morgantown, West Virginia 26506-9159, USA. rhoeldtke@hsc.wvu.edu
authors.forename: Robert D
authors.initials: RD
authors.lastname: Hoeldtke
authors.forename: Kimberly D
authors.initials: KD
authors.lastname: Bryner
authors.forename: Daniel R
authors.initials: DR
authors.lastname: McNeill
authors.forename: Sarah S
authors.initials: SS
authors.lastname: Warehime
authors.forename: Knox
authors.initials: K
authors.lastname: Van Dyke
authors.forename: Gerald
authors.initials: G
authors.lastname: Hobbs
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000097:blood* / Q000188:drug therapy*; D015237:Dinoprost / Q000031:analogs & derivatives*; D028441:F2-Isoprostanes / Q000097:blood; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D007328:Insulin / Q000008:administration & dosage*; D007515:Islets of Langerhans / Q000378:metabolism; D008137:Longitudinal Studies; D008297:Male; D009566:Nitrates / Q000097:blood; D009573:Nitrites / Q000097:blood; D018384:Oxidative Stress; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000097:blood
chemical_list: D015237:Dinoprost; D028441:F2-Isoprostanes; D006442:Glycated Hemoglobin; D007328:Insulin; D009566:Nitrates; D009573:Nitrites; D014443:Tyrosine; C075750:8-epi-prostaglandin F2alpha; C002744:3-nitrotyrosine