[Transendothelium electric resistance and permeability on bovine serum album of cochlear microvascular endothelial cells from guinea pig].
OBJECTIVE
To study the dynamic changes and the peak phases of transendothelial electric resistance (TEER) during the growth of cochlear microvascular endothelial cells (CMEC) in guinea pigs and the monolayer endothelial cells permeability on radioiodinated 125I bovine serum album (RISA).


METHODS
1. Establish a model in vitro for the permeability of CMEC using small pool technique. 2. Research the model's TEER and its permeability on RISA.


RESULTS
1. The TEER changed dynamically and reached the peak about seven days after inoculation of culture cells at 5 x 10(4)/cm2 level during the CMEC proliferated. The peak values of TEER was 118.9 +/- 18.5 omega/cm2; 2. After RISA administration, the Bake-time curve took on an ascending parabola, while within 90 min appeared like a straight line.


CONCLUSIONS
The TEER and the Varity curve on RISA reveals the permeability of CMEC in vitro.
Wei
Yunjun
Y
Department of Otorhinolaryngology, Southwest Hospital, Third Military Medical University, Chongqing 400038.
Zhang
Xueyuan
X
D000818:Animals; D002199:Capillary Permeability; D002417:Cattle; D003051:Cochlea / Q000166:cytology*; D017097:Electric Impedance; D004730:Endothelium, Vascular / Q000502:physiology*; D006168:Guinea Pigs; D066298:In Vitro Techniques; D012709:Serum Albumin / Q000493:pharmacokinetics
D012709:Serum Albumin