Localization of CD4 and CCR5 in living cells.
The events preceding human immunodeficiency virus fusion and entry are influenced by the concentration and distribution of receptor and coreceptor molecules on the cell surface. However, the extent to which these proteins colocalize with one another in the cell membrane remains unclear. Using high-resolution deconvolution fluorescent microscopy of living cells, we found that both CD4 and CCR5 accumulate in protruding membrane structures containing actin and ezrin. Although CD4 and CCR5 extensively colocalize in these structures, they do not exist in a stable complex.
Steffens
Carolyn M
CM
Department of Microbiology and Immunology, University of Illinois at Chicago, Chicago, Illinois 60612, USA.
Hope
Thomas J
TJ
D000199:Actins / Q000378:metabolism; D015704:CD4 Antigens / Q000378:metabolism*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D003598:Cytoskeletal Proteins; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D010750:Phosphoproteins / Q000378:metabolism; D019713:Receptors, CCR5 / Q000378:metabolism*; D015728:Receptors, HIV / Q000378:metabolism*; D013601:T-Lymphocytes; D000099149:Ezrin
D000199:Actins; D015704:CD4 Antigens; D003598:Cytoskeletal Proteins; D010750:Phosphoproteins; D019713:Receptors, CCR5; D015728:Receptors, HIV; D000099149:Ezrin