[Protective effect of dansheng injection on experimental rabbits' spinal cord injury].
OBJECTIVE
To investigate the protective effect of Dansheng injection on experimental rabbits' in spinal cord injury.


METHODS
Thirty-two New Zealand rabbits were impacted between T12-T1 with a force of 150 g.cm-1 (10 g x 15 cm). The rabbits were randomly divided into the injury group, monitor group, L-NAME group and Dansheng group. The blood was obtained at 0 h, 4 h, 6 h to separate the serum at the end the injured spinal cord. Cord tissues were obtained for histological examination. Methyl-di-aldehyde (MDA), superoxide dismutase (SOD) and nitro oxide (NO) from the serum and spinal tissues homogenated were tested.


RESULTS
MDA and NO increased in the injury group compared with the others, but SOD significantly decreased in the injury group compared with the others. Histological examination showed that neurons were degenerated and necrosed. The injury group displayed serious changes while the Dansheng group less changes. iNOS expressed in all the groups.


CONCLUSIONS
Dansheng injection has some protective effect on spinal cord injury in the early stage.
Ni
Jiang-dong
JD
Department of Orthopedic Surgery, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Ding
Ren-kui
RK
Lü
Guo-hua
GH
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism; D011817:Rabbits; D011897:Random Allocation; D027561:Salvia miltiorrhiza / Q000737:chemistry; D013119:Spinal Cord Injuries / Q000188:drug therapy / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors
D004365:Drugs, Chinese Herbal; D016166:Free Radical Scavengers; D018696:Neuroprotective Agents; D009569:Nitric Oxide; D013482:Superoxide Dismutase