Analysis of water extracts from airborne dust samples by capillary isotachophoresis.
Application of capillary isotachophoresis (CITP) for the analysis of water extracts of the dust samples collected in different periods in air-filtration devices in Prague car traffic tunnels and in Parisian metro station is presented. The extracts were analyzed in cationic mode with a leading electrolyte (LE) of 10 mM KOH, 25 mM acetic acid, pH 4.4, and a terminating electrolyte (TE) of 10 mM beta-alanine, adjusted to pH 4.4 with acetic acid, and in anionic mode with LE 10 mM HCl, 20 mM histidine, pH 5.8 and TE 10 mM 2-(N-morpholino)ethanesulphonic acid, pH 3.7. Extracted amounts of UV-absorbing substances, including pollen allergens and organic pollutants, the number of the found components and concentrations of some inorganic ions (e.g. Cl-, K+, Na+, Ca2+) in the dust samples were determined. It was found that the extracted amounts of anionic components and their number were much higher than those of cationic components. Significant differences have been found in the analyses of the extracts of different origin. Much more material and more components were present in the extracts of samples from the pollen-rich period than from the pollen-free period, especially in anionic CITP mode.
Sázelová
Petra
P
Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo 2, CZ-166 10 Prague 6, Czech Republic.
Kasicka
Václav
V
Koval
Dusan
D
Kilár
Ferenc
F
Knopp
Dietmar
D
Peltre
Gabriel
G
D000393:Air Pollutants / Q000032:analysis*; D004391:Dust; D004586:Electrophoresis / Q000379:methods*; D014867:Water
D000393:Air Pollutants; D004391:Dust; D014867:Water