title: Exopolysaccharide and kestose production by Lactobacillus sanfranciscensis LTH2590.
abstract: The effect was investigated of sucrose concentration on sucrose metabolism and on the formation of exopolysaccharide (EPS) by Lactobacillus sanfranciscensis LTH2590 in pH-controlled fermentations with sucrose concentrations ranging from 20 to 160 g liter(-1). The EPS production increased and the relative sucrose hydrolysis activity decreased by increasing the sucrose concentration in the medium. The carbon recovery decreased from 95% at a sucrose concentration of 30 g liter(-1) to 58% at a sucrose concentration of 160 g liter(-1) because of the production of an unknown metabolite by L. sanfranciscensis. This metabolite was characterized as a fructo-oligosaccharide. The oligosaccharide produced by L. sanfranciscensis was purified and characterized as a trisaccharide with a glucose/fructose ratio of 1:2. The comparison of the retention time of this oligosaccharide and that of pure oligosaccharide standards using two different chromatography methods revealed that the oligosaccharide produced by L. sanfranciscensis LTH2590 is 1-kestose. Kestose production increased concomitantly with the initial sucrose concentration in the medium.
authors.affiliation: Lehrstuhl für Technische Mikrobiologie, Technische Universität München, Freising, Germany.
authors.forename: Maher
authors.initials: M
authors.lastname: Korakli
authors.forename: Melanie
authors.initials: M
authors.lastname: Pavlovic
authors.forename: Michael G
authors.initials: MG
authors.lastname: Gänzle
authors.forename: Rudi F
authors.initials: RF
authors.lastname: Vogel
mesh_terms: D003470:Culture Media; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000254:growth & development / Q000378:metabolism*; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D013395:Sucrose / Q000378:metabolism*
chemical_list: D003470:Culture Media; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; C116580:fructooligosaccharide; D013395:Sucrose