title: [An experimental study on the role of indirect antigen presentation pathway on the immunogenetic of epidermal cells].
abstract: OBJECTIVE
  To explore the role of indirect antigen presentation pathway on the immunogenecity of epidermal cells.
  
  
  METHODS
  Human epidermal cells (HEC), allogeneic human peripheral blood lymphocytes (PBL) and mononuclear cells (PBM, including monocytes) were isolated and cultured in vitro. HECs were transfected by human-originated CTLA4Ig-adenovirus vector. The CTLA4Ig expression was observed. Allogeneic PBLs or PBMs were added to the transfected and non-transfected HECs with simple cultured PBLs and PBMs as the control. The proliferation of PBL and PBM was determined by (3)H-TdR incooperation.
  
  
  RESULTS
  HECs could be successfully transfected by CTLA4Ig-adenovirus vector and expressed corresponding proteins. The non-transfected HECs could stimulate slight proliferation of allogeneic PBLs (P < 0.05) and stimulate remarkable proliferation of PBMs (including monocytes) (P < 0.05). The proliferation reaction of PBLs and PBMs decreased significantly (P < 0.05) after being stimulated by HEC which was modulated by CTLA4Ig genes.
  
  
  CONCLUSIONS
  Indirect antigen presentation pathway might play important roles in the HEC immunogenicity which could be evidently inhibited by CTLA4Ig.
authors.affiliation: Institute of Burn Research, Southwestern Hospital, The Third Military Medical University, Institute of Organ Transplantation Research of Chongqing City. Chongqing, 400038. P.R. China.
authors.forename: Xi-Wei
authors.initials: XW
authors.lastname: Chen
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.forename: Qi-Zhi
authors.initials: QZ
authors.lastname: Luo
authors.forename: Gao-Xing
authors.initials: GX
authors.lastname: Luo
authors.forename: Xi-Hua
authors.initials: XH
authors.lastname: Wang
authors.forename: Shao-Xuan
authors.initials: SX
authors.lastname: Yi
authors.forename: Wei-Feng
authors.initials: WF
authors.lastname: He
authors.forename: Bing
authors.initials: B
authors.lastname: Ma
authors.forename: Ru-Peng
authors.initials: RP
authors.lastname: Wang
authors.forename: Jin
authors.initials: J
authors.lastname: Tang
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D017951:Antigen Presentation / Q000276:immunology* / Q000502:physiology; D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000235:genetics / Q000276:immunology; D060908:CTLA-4 Antigen; D002455:Cell Division / Q000276:immunology; D002478:Cells, Cultured; D018920:Coculture Techniques; D000078404:Epidermal Cells; D004817:Epidermis / Q000276:immunology* / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology; D015398:Signal Transduction; D014162:Transfection
chemical_list: D015703:Antigens, CD; D000943:Antigens, Differentiation; D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human