Tubulin diversity in trophozoites of Giardia lamblia.
Giardia lamblia is a diplomonad that parasitizes the small intestine of vertebrates. The trophozoite is multiflagellar and its cytoskeleton presents a complex organization of microtubular structures. One of these, the adhesive disk, consists of a microtubular spiral. The median body, whose function is not yet determined, is also composed by microtubules. The cell has eight flagella and two microtubule sheets, known as the funis. In this study we used several antibodies and immunofluorescence microscopy to help in the characterization of these structures. We observed that Giardia tubulin reacts with antibodies raised against very distinct immunogens. The antibodies used were against: (1) alpha-tubulin from chicken embryo brain, Trypanosoma brucei, sea urchin sperm, Paramecium, acetylated alpha-tubulin from Paramecium, and tyrosinated alpha-tubulin, (2) beta-tubulin from chicken embryo brain and Physarum polycephalum, and (3) an antibody with specificity to beta-tubulin having as immunogen the FtsZ bacterial protein. Each cytoskeletal structure of Giardia presented a distinct pattern of labeling by the several antibodies used. These data indicate that even being considered one of the most ancient of organisms, Giardia shares similarities (at least in relation to tubulin) with other organisms. They also open some questions about the organization and composition of its microtubular structures.
Campanati
Loraine
L
Instituto de Biofísica Carlos Chagas Filho, Laboratório de Ultraestrutura Celular Hertha Meyer, CCS, Universidade Federal do Rio de Janeiro, Ilha do Fundão, 21940-900 Rio de Janeiro, Brazil.
Troester
Helmut
H
Monteiro-Leal
Luiz Henrique
LH
Spring
Herbert
H
Trendelenburg
Michael F
MF
de Souza
Wanderley
W
D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D000953:Antigens, Protozoan / Q000032:analysis; D016829:Giardia lamblia / Q000737:chemistry* / Q000254:growth & development; D066298:In Vitro Techniques; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000737:chemistry*; D014404:Tubulin / Q000032:analysis* / Q000276:immunology
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D014404:Tubulin