Partial oncogenic transformation of chicken embryo fibroblasts by Jun dimerization protein 2, a negative regulator of TRE- and CRE-dependent transcription.
Jun dimerization protein 2 (JDP2) was identified as a bZIP protein that forms dimers with Jun proteins. JDP2 represses transcriptional activation of reporter constructs containing 12-O-tetradecanoylphorbol 13-acetate (TPA)-responsive elements (TRE) or cyclic AMP responsive elements (CRE). JDP2, overexpressed by the avian retroviral vector RCAS, induces partial oncogenic transformation of chicken embryo fibroblasts. JDP2-expressing cells form multilayered foci in monolayer cultures but do not show anchorage-independent growth. Both the carboxyl and the amino terminus of JDP2 are required for the transforming activity. Chimeric constructs of JDP2 carrying the leucine zipper domain of Fos, GCN4 or EB1 fail to transform CEF. The leucine zipper of Fos mediates only heterodimerization; it cannot homodimerize. In contrast, the leucine zippers of GCN4 and of EB1 exclusively homodimerize and do not form dimers with other bZip proteins. The results with the JDP2 chimeras suggest that the JDP2 homodimer and the JDP2/Jun heterodimer (or other bZip heterodimers formed with the Fos leucine zipper) are nontransforming, leaving as possible transforming combination the JDP2/Fos heterodimer. The unexpected transforming activity of a negative regulator of TRE- and CRE-dependent transcription raises an important question concerning the mechanisms of transformation by the related bZIP proteins Jun and Fos that address the same target sequences.
Blazek
Erik
E
Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA.
Wasmer
Sibylle
S
Kruse
Ulrich
U
Aronheim
Ami
A
Aoki
Masahiro
M
Vogt
Peter K
PK
D051698:Activating Transcription Factor 2; D000818:Animals; D002471:Cell Transformation, Neoplastic; D002642:Chick Embryo; D017362:Cyclic AMP Response Element-Binding Protein / Q000235:genetics*; D005347:Fibroblasts; D015972:Gene Expression Regulation, Neoplastic; D012097:Repressor Proteins / Q000502:physiology*; D020218:Response Elements / Q000235:genetics; D013755:Tetradecanoylphorbol Acetate / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics*; D014162:Transfection
D051698:Activating Transcription Factor 2; D017362:Cyclic AMP Response Element-Binding Protein; D012097:Repressor Proteins; D014157:Transcription Factors; D013755:Tetradecanoylphorbol Acetate