Lignin degradation by Agaricus bisporus accounts for a 30% increase in bioavailable holocellulose during cultivation on compost.
The common mushroom Agaricus bisporus is a non-white rot saphrophytic fungus that can degrade lignin to free and utilize holocellulose embedded in fermented straw as present in compost. A new method is described to estimate the actual amount of bioavailable holocellulose in 3.8 kg compost cultures spawned with A. bisporus Horst U1 prior to and during a cultivation with two cycles of mushroom harvesting. The method shows that the initial amount of bioavailable holocellulose per culture, accounting for 130 +/- 22 g, is lower than the total holocellulose consumption by A. bisporus accounting for 182 +/- 15 g. This difference is explained by a 30% increase in bioavailable holocellulose. The increase is caused by the degradation of 95 +/- 3 g of holocellulose-shielding lignin. The results are discussed within the scope of the A. bisporus mushroom yield and lignin degradation by white rot fungi during growth on lignocellulose-containing materials.
ten Have
Rimko
R
Wageningen University and Research Centre, Laboratory for Microbiology, Section Fungal Genomics, Dreijenlaan 2, 6703 HA Wageningen, The Netherlands. Rimko.tenHave@wur.nl
Wijngaard
Hilde
H
Ariës-Kronenburg
Nicole A E
NA
Straatsma
Gerben
G
Schaap
Peter J
PJ
D000364:Agaricus / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002482:Cellulose / Q000032:analysis / Q000378:metabolism*; D008031:Lignin / Q000378:metabolism*
D002482:Cellulose; D008031:Lignin