title: The Lymantria dispar nucleopolyhedrovirus contains the capsid-associated p24 protein gene.
abstract: During the course of investigations on a wild-type strain of Lymantria dispar multinucleocapsid nucleopolyhedrovirus (LdMNPV), a region of the viral genome was analyzed and found to contain 697 bp that is lacking in the sequenced strain (5-6) of LdMNPV (Kuzio et al., Virology 253, 17-34, 1999). The sequenced strain of LdMNPV contains a mutation in the 25 K few polyhedra (FP) gene, and exhibits the phenotype of a FP mutant. The additional sequence was located at approximately 81.4 map units within the viral genome, and was found in 10 different wild-type LdMNPV genotypic variants analyzed. Since the additional sequence wasfound in all wild-type virus strains analyzed, this sequence should be included in the representative LdMNPV genome. Sequence analysis of the genomic region containing the additional sequences revealed the presence of a homologue of the Autographa californica MNPV capsid-associated p24 gene (ORF 129). This gene, absent in LdMNPV isolate 5-6, is also present in the Orgyia pseudotsugata MNPV, Bombyx mori NPV, Spodoptera exigua MNPV, S. litura MNPV, Mamestra configurata MNPV, Helicoverpa armigera SNPV, H. zea SNPV, Buzura suppressaria SNPV, Xestia c-nigrum granulovirus, Plutella xylostella GV, and Cydia pomonella GV.
authors.affiliation: USDA Forest Service, 359 Main Road, Delaware, OH 43015, USA. jslavicek@fs.fed.us
authors.forename: James M
authors.initials: JM
authors.lastname: Slavicek
authors.forename: Nancy
authors.initials: N
authors.lastname: Hayes-Plazolles
mesh_terms: D000818:Animals; D002213:Capsid; D005814:Genes, Viral; D014644:Genetic Variation; D016679:Genome, Viral; D005838:Genotype; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009036:Moths / Q000821:virology*; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D016366:Open Reading Frames; D012150:Polymorphism, Restriction Fragment Length; D013045:Species Specificity; D014764:Viral Proteins / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics*
chemical_list: D014764:Viral Proteins; D015678:Viral Structural Proteins