Development of an indirect competitive enzyme-linked immunosorbent assay to detect extracellular polymeric substances (EPS) secreted by the marine stromatolite-forming cyanobacteria, Schizothrix sp.
An indirect competitive enzyme-linked immunosorbent assay was developed using polyclonal antibody to detect extracellular polymeric secretions (EPS) produced by the marine stromatolite-forming cyanobacteria, Schizothrix sp. The cross-reactivity of this assay with other EPSs and polymers were low (< 0.5%). This assay can detect Schizothrix sp. EPS as low as 0.5 ng/mL. Intra-assay and inter-assay comparisons showed that coefficient variations were low, ranging from 3.34 to 10.30% and from 6.30 to 12.8%, respectively, for standards between 2 and 1000 ng/mL. Also, the seawater matrix effect was negligible. Our results indicated that this assay is a useful tool for quantification of Schizothrix sp. EPS in a range of ng/mL.
Kawaguchi
Tomohiro
T
Department of Environmental Health Sciences, The Norman J. Arnold School of Public Health, University of South Carolina, Columbia, South Carolina 29208, USA. kawaguch@gwm.sc.edu
Sayegh
Husain Ala
HA
Decho
Alan W
AW
D003429:Cross Reactions; D000458:Cyanobacteria / Q000737:chemistry / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards; D011108:Polymers / Q000032:analysis* / Q000302:isolation & purification; D015203:Reproducibility of Results; D013045:Species Specificity; D014871:Water Microbiology
D011108:Polymers