title: DC-SIGN (CD209) mediates dengue virus infection of human dendritic cells.
abstract: Dengue virus is a single-stranded, enveloped RNA virus that productively infects human dendritic cells (DCs) primarily at the immature stage of their differentiation. We now find that all four serotypes of dengue use DC-SIGN (CD209), a C-type lectin, to infect dendritic cells. THP-1 cells become susceptible to dengue infection after transfection of DC-specific ICAM-3 grabbing nonintegrin (DC-SIGN), or its homologue L-SIGN, whereas the infection of dendritic cells is blocked by anti-DC-SIGN antibodies and not by antibodies to other molecules on these cells. Viruses produced by dendritic cells are infectious for DC-SIGN- and L-SIGN-bearing THP-1 cells and other permissive cell lines. Therefore, DC-SIGN may be considered as a new target for designing therapies that block dengue infection.
authors.affiliation: Division of Retrovirology, Walter Reed Army Institite of Research and Henry M. Jackson Foundation for the Advancement of Military Medicine Diseases, Rockville, MD 20850, USA. mmarovich@hivresearch.org
authors.forename: Boonrat
authors.initials: B
authors.lastname: Tassaneetrithep
authors.forename: Timothy H
authors.initials: TH
authors.lastname: Burgess
authors.forename: Angela
authors.initials: A
authors.lastname: Granelli-Piperno
authors.forename: Christine
authors.initials: C
authors.lastname: Trumpfheller
authors.forename: Jennifer
authors.initials: J
authors.lastname: Finke
authors.forename: Wellington
authors.initials: W
authors.lastname: Sun
authors.forename: Michael A
authors.initials: MA
authors.lastname: Eller
authors.forename: Kovit
authors.initials: K
authors.lastname: Pattanapanyasat
authors.forename: Suttipant
authors.initials: S
authors.lastname: Sarasombath
authors.forename: Deborah L
authors.initials: DL
authors.lastname: Birx
authors.forename: Ralph M
authors.initials: RM
authors.lastname: Steinman
authors.forename: Sarah
authors.initials: S
authors.lastname: Schlesinger
authors.forename: Mary A
authors.initials: MA
authors.lastname: Marovich
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000276:immunology / Q000502:physiology*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000821:virology*; D003715:Dengue / Q000145:classification; D003716:Dengue Virus / Q000145:classification / Q000502:physiology*; D005434:Flow Cytometry; D006801:Humans; D037181:Lectins, C-Type / Q000276:immunology / Q000502:physiology*; D011956:Receptors, Cell Surface / Q000276:immunology / Q000502:physiology*; D011991:Receptors, Virus / Q000502:physiology; D012703:Serotyping; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D037181:Lectins, C-Type; D011956:Receptors, Cell Surface; D011991:Receptors, Virus; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin