title: Effect of chlorpromazine free radical on tyrosine hydroxylase of bovine adrenal medulla.
abstract: The effect of preincubation with chlorpromazine and chlorpromazine free radical on tyrosine hydroxylase activity was studied. Chlorpromazine free radical inhibited the enzyme activity to a greater extent than chlorpromazine. More than a two-fold decrease in the enzyme activity was observed following the preincubation of the enzyme with chlorpromazine free radical. This observation may suggest interaction between chlorpromazine free radical and -SH group located on the active site of the apoenzyme. Another interaction between chlorpromazine free radical and the coenzyme (DMPH4) was confirmed from spectral studies.
authors.affiliation: Medical Research Institute, Alexandria University, Alexandria.
authors.forename: M H
authors.initials: MH
authors.lastname: Mostafa
authors.forename: S M
authors.initials: SM
authors.lastname: el-Sewedy
authors.forename: Z
authors.initials: Z
authors.lastname: Rakonczay
mesh_terms: D000313:Adrenal Medulla / Q000187:drug effects / Q000201:enzymology*; D000818:Animals; D014150:Antipsychotic Agents / Q000737:chemistry / Q000494:pharmacology*; D002417:Cattle; D002746:Chlorpromazine / Q000737:chemistry / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005609:Free Radicals; D066298:In Vitro Techniques; D014446:Tyrosine 3-Monooxygenase / Q000037:antagonists & inhibitors*
chemical_list: D014150:Antipsychotic Agents; D004791:Enzyme Inhibitors; D005609:Free Radicals; D014446:Tyrosine 3-Monooxygenase; D002746:Chlorpromazine