Affinity electrochromatography of acidic drugs using a liposome-modified capillary.
Liposomes can be effectively deposited on the inner surface of a capillary wall by flushing the electrophoretic system with a liposome suspension followed by air-drying of the capillary and removal of the excess of loosely bound liposomes by a 0.1 M NaOH wash. It was demonstrated that capillaries prepared in this way could be used for studies of analyte (drug)-liposome binding. The results were expressed as free binding energy changes [delta(deltaG0)] relatively to an arbitrarily selected standard (acetylsalicylic acid). The results were compared to [delta(deltaG0)] changes obtained from binding studies effected by capillary electrophoresis using a stable liposome plug in a capillary with minimized endoosmotic flow. Good agreement of data reported in the literature (without correction for the residual endoosmotic flow), our previous data obtained in a similar way (however, after the correction for the residual endoosmotic flow) and data obtained by the immobilized liposome affinity electrochromatography reported in this communication was achieved.
Manetto
Giulia
G
GlaxoSmithKline, Department of Pharmacology and Analytical Chemistry, Verona, Italy.
Silvana Bellini
Maria
M
Deyl
Zdenek
Z
D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation*; D006863:Hydrogen-Ion Concentration; D008081:Liposomes; D004364:Pharmaceutical Preparations / Q000302:isolation & purification*; D013816:Thermodynamics
D008081:Liposomes; D004364:Pharmaceutical Preparations