High levels of apoptosis induced by total body irradiation in mice fed a low protein-low vitamin E diet.
Apoptosis in response to total body irradiation (TBI) in mice fed four kinds of diets was assessed. Male mice were fed a low protein-low vitamin E diet, a low protein-basal vitamin E diet, a basal protein-low vitamin E diet or a basal protein-basal vitamin E diet for 2 weeks, then received TBI at a dose of 0, 1 or 4 Gy, and were sacrificed 4 h after TBI. Apoptosis was assessed according to the appearance of DNA fragmentation patterns [DNA laddering and positive in situ end-labeling by terminal transferase (TUNEL) assay] and apoptotic index (AI). Electrophoresis of DNA from the spleen in the groups that did not receive TBI showed no cleavage, whereas that of DNA in the groups with 4 Gy of TBI displayed a ladder pattern, indicative of internucleosomal cleavage characteristic of apoptosis. In the groups with 4 Gy of TBI, apoptosis was enhanced in the low protein-low vitamin E group.
Shin
S J
SJ
Department of Food Science Research for Health, National Institute of Health and Nutrition, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8636, Japan. mhjrm@nih.go.jp
D000818:Animals; D017209:Apoptosis; D004249:DNA Damage; D004032:Diet; D004044:Dietary Proteins; D008297:Male; D051379:Mice; D018384:Oxidative Stress; D011488:Protein Deficiency / Q000150:complications*; D014811:Vitamin E Deficiency / Q000150:complications*; D014916:Whole-Body Irradiation
D004044:Dietary Proteins