A cDNA encoding a putative lipid transfer protein expressed in sunflower seeds.
Based on the N-terminal sequence of a sunflower antifungal protein, a full length cDNA (Ha-LTP5) encoding a putative lipid transfer protein from sunflower seeds was cloned using a RT-PCR based strategy. However, the sequence of the deduced protein is not identical to that of the antifungal protein previously isolated. The nucleotide sequence presents an ORF of 116 amino acids with a putative signal peptide, thus encoding a mature protein of 90 amino acids that is basic and hydrophobic. In contrast to the pattern of expression described for most LTP-like genes from dicots, Northern blot analyses detected constitutive expression of Ha-LTP5 in seeds, but not in aerial parts of sunflower plants.
Regente
Mariana
M
Instituto de Investigaciones Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Casilla de Correo 1245, 7600 Mar del Plata, Argentina.
de la Canal
Laura
L
D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000737:chemistry; D052179:Antigens, Plant; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics*; D006368:Helianthus / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D012639:Seeds / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D000935:Antifungal Agents; D052179:Antigens, Plant; D002352:Carrier Proteins; D018076:DNA, Complementary; D018744:DNA, Plant; D010940:Plant Proteins; C511920:lipid transfer proteins, plant