Solid-phase synthesis of positively charged deoxynucleic guanidine (DNG) oligonucleotide mixed sequences.
Positively charged DNG oligonucleotide mixed sequences containing A/T bases were prepared by solid-phase synthesis. Synthesis proceeds in 3'-->5' direction and involves coupling of 3'-Fmoc protected thiourea in the presence of HgCl(2)/TEA with the corresponding 5'-amine of the growing oligo chain. DNG binding characteristics with complementary DNA and with itself have been evaluated.
Reddy
Putta Mallikarjuna
PM
Department of Chemistry and Biochemistry, University of California at Santa Barbara, CA 93106, USA.
Bruice
Thomas C
TC
D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry; D006146:Guanidines / Q000737:chemistry*; D007202:Indicators and Reagents; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D011687:Purines / Q000737:chemistry; D011743:Pyrimidines / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
D006146:Guanidines; D007202:Indicators and Reagents; D009841:Oligonucleotides; D011687:Purines; D011743:Pyrimidines; D004247:DNA