[Expression of iNOS and activities of iNOS and cNOS in right and left ventricles of the isolated heart during ischemia and reperfusion].
Isolated using Langendorff technique hearts of male Wistar rats were ischemized for 30 min and reperfused for 40 min. Both iNOS expression determined by immunoblotting, and its activity shown by a modified Griess method have been found to predominate in the right ventricle over the left one, as compared to cNOS activity which prevailed in the left ventricle. The latter significantly diminished after an ischemic injury in the left ventricle. On the contrary, an expression of iNOS and the total NOS activities increased progressively after ischemia and postischemic reperfusion; all the values in the right ventricle were higher than in the left one. The high level of iNOS expression in the right ventricle in a postischemic period decreased during reperfusion, although an activity of the enzyme went on elevating. These data can give an evidence for different regulation of NO synthesis in right or left ventricles of the heart, including normal conditions or ischemia.
Harmatina
O Iu
OIu
A.A. Bogomoletz Institute of Physiology of Ukraine, Kiev.
Portnychenko
A H
AH
Moĭbenko
O O
OO
Kotsiuruba
A V
AV
Bukhanevych
O M
OM
D000818:Animals; D015153:Blotting, Western; D006352:Heart Ventricles / Q000201:enzymology; D066298:In Vitro Techniques; D008297:Male; D017202:Myocardial Ischemia / Q000150:complications / Q000201:enzymology*; D015428:Myocardial Reperfusion Injury / Q000201:enzymology* / Q000209:etiology; D019001:Nitric Oxide Synthase / Q000096:biosynthesis* / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; D051381:Rats; D017208:Rats, Wistar
D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; C496318:Nos2 protein, rat; C496316:Nos3 protein, rat