Studies on the influence of DNA repair on radiosensitivity in prostate cell lines.
The relationship between radiosensitivity and DNA repair was investigated in six human prostate cell lines, 1542-NPTX, BPH-1, 1542-CP(3)TX, 1532-CP(2)TX, 1535-CP(1)TX and LNCaP. Except for LNCaP, these cell lines are new and were derived from primary prostate tumours and normal non-tumourigenic prostate tissue. Cell survival was assessed by clonogenic assay. DNA damage was determined in non-synchronised cells by constant-field gel electrophoresis, and expressed as the fraction of DNA released. For initial damage, cells were embedded in agarose and irradiated on ice with 0-100 Gy (60)Co gamma-irradiation. Residual DNA damage was measured after 2 h and 20 h of repair. Radiosensitivity, given as the mean inactivation dose, was found to vary between 1.62 and 2.77 Gy. We found that radiosensitivity significantly correlates with the 2 h DNA repair component, giving a correlation coefficient of 0.92 ( P=0.009). In the cell lines examined here the 2 h repair component emerges as an indicator of radiosensitivity.
Serafin
Antonio M
AM
Department of Radiation Oncology, Faculty of Health Sciences and Tygerberg Hospital, PO Box 19063, Tygerberg 7505, South Africa.
Akudugu
John M
JM
Böhm
Lothar
L
D045744:Cell Line, Tumor / Q000166:cytology / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D004247:DNA / Q000528:radiation effects*; D004260:DNA Repair; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D008297:Male; D011467:Prostate / Q000166:cytology*; D011470:Prostatic Hyperplasia; D011471:Prostatic Neoplasms; D011836:Radiation Tolerance
D004247:DNA