Evidence for lipopolysaccharide-induced differentiation of RAW264.7 murine macrophage cell line into dendritic like cells.
Effect of lipopolysaccharide (LPS) on RAW264.7 macrophage cell line was studied. LPS-treated RAW264.7 cells increased in cell size and acquired distinct dendritic morphology. At the optimal dose of LPS (1 mg/ml), almost 70% RAW264.7 cells acquired dendritic morphology. Flow cytometric studies indicate that the cell surface markers known to be expressed on dendritic cells and involved in antigen presentation and T cell activation (B 7.1, B 7.2, CD40, MHC class II antigens and CD1d) were also markedly upregulated on LPS-treated RAW 264.7 cells. Our results suggest the possibility that LPS by itself could constitute a sufficient signal for differentiation of macrophages into DC-like cells.
Saxena
Rajiv K
RK
School of Life Sciences, Jawaharlal Nehru University, New Delhi 110 067, India. rksaxena@mail.jnu.ac.in
Vallyathan
Val
V
Lewis
Daniel M
DM
D000818:Animals; D017951:Antigen Presentation; D018949:Antigens, CD1 / Q000276:immunology; D015415:Biomarkers; D019013:CD40 Antigens / Q000276:immunology; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008213:Lymphocyte Activation; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects*; D051381:Rats; D013601:T-Lymphocytes / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
D018949:Antigens, CD1; D015415:Biomarkers; D019013:CD40 Antigens; D000949:Histocompatibility Antigens Class II; D008070:Lipopolysaccharides