title: Solid-phase synthesis of positively charged deoxynucleic guanidine (DNG) oligonucleotide mixed sequences.
abstract: Positively charged DNG oligonucleotide mixed sequences containing A/T bases were prepared by solid-phase synthesis. Synthesis proceeds in 3'-->5' direction and involves coupling of 3'-Fmoc protected thiourea in the presence of HgCl(2)/TEA with the corresponding 5'-amine of the growing oligo chain. DNG binding characteristics with complementary DNA and with itself have been evaluated.
authors.affiliation: Department of Chemistry and Biochemistry, University of California at Santa Barbara, CA 93106, USA.
authors.forename: Putta Mallikarjuna
authors.initials: PM
authors.lastname: Reddy
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Bruice
mesh_terms: D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry; D006146:Guanidines / Q000737:chemistry*; D007202:Indicators and Reagents; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D011687:Purines / Q000737:chemistry; D011743:Pyrimidines / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
chemical_list: D006146:Guanidines; D007202:Indicators and Reagents; D009841:Oligonucleotides; D011687:Purines; D011743:Pyrimidines; D004247:DNA