title: Quantitative bioanalysis of enantiomeric drugs using capillary electrophoresis and electrospray mass spectrometry.
abstract: A novel assay method for an enantiomeric pair of drugs has been developed using a combination of capillary electrophoresis and electrospray tandem mass spectrometry connected with a homemade interface. Accurate quantification was demonstrated in plasma from 0.25 to 50 microg/ml. A liquid-liquid sample preparation technique allowed improvement in the quantitation limit to 10 ng/ml. Variables for the enantiomeric separation, including chiral selective reagent, organic solvents, buffer and acid concentration as well as injection technique, were optimized. This assay proved adequate for analysis of neat, spiked plasma, and plasma from a pharmacological study of the drug enantiomers.
authors.affiliation: Bioanalytical Core Group, Pfizer Global R&D, 2800 Plymouth Road, Ann Arbor, MI 48105, USA. erick.kindt@pfizer.com
authors.forename: Erick K
authors.initials: EK
authors.lastname: Kindt
authors.forename: Stephen
authors.initials: S
authors.lastname: Kurzyniec
authors.forename: Shau-Chun
authors.initials: SC
authors.lastname: Wang
authors.forename: Gregory
authors.initials: G
authors.lastname: Kilby
authors.forename: David T
authors.initials: DT
authors.lastname: Rossi
mesh_terms: D000818:Animals; D001643:Bridged Bicyclo Compounds / Q000097:blood*; D019075:Electrophoresis, Capillary / Q000379:methods / Q000592:standards; D000882:Haplorhini; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods / Q000592:standards; D013237:Stereoisomerism
chemical_list: D001643:Bridged Bicyclo Compounds; C479718:PD0149388; C479720:PD0217015; C479719:PD0217016