title: Sarcoglycans of the zebrafish: orthology and localization to the sarcolemma and myosepta of muscle.
abstract: The zebrafish is an established model of vertebrate development and is also receiving increasing attention in terms of human disease modelling. In order to provide experimental support to realize this modelling potential, we report here the identification of apparent orthologues of many critical members of the dystrophin-associated glycoprotein complex (DGC) that have been implicated in a diverse range of neuromuscular disorders. In addition, immunohistochemical studies show the localization of the DGC to the sarcolemma of adult zebrafish muscle and in particular the myosepta. Together, these data suggest that the DGC in adult zebrafish may play a highly conserved functional role in muscle architecture that, when disrupted, could offer insight into human neuromuscular disease processes.
authors.affiliation: Molecular Genetics and Development Group, School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand.
authors.forename: Stephen P
authors.initials: SP
authors.lastname: Chambers
authors.forename: Louise V B
authors.initials: LV
authors.lastname: Anderson
authors.forename: Giselle M
authors.initials: GM
authors.lastname: Maguire
authors.forename: Andrew
authors.initials: A
authors.lastname: Dodd
authors.forename: Donald R
authors.initials: DR
authors.lastname: Love
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003598:Cytoskeletal Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D049030:Dystroglycans; D006801:Humans; D007150:Immunohistochemistry; D008562:Membrane Glycoproteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000648:ultrastructure; D010802:Phylogeny; D012508:Sarcolemma / Q000378:metabolism / Q000648:ultrastructure*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015027:Zebrafish
chemical_list: D003598:Cytoskeletal Proteins; C483081:DAG1 protein, human; D008562:Membrane Glycoproteins; D009124:Muscle Proteins; D049030:Dystroglycans