title: Hematopoietic progenitor colony formation in the immunopathogenesis of the autoimmune disorder in MRL/MpJ-lpr mice.
abstract: Lymphocyte proliferation and apoptosis at different stages of the development of the autoimmune disorder in MRL/MpJ-lpr mice was studied. Hematopoietic progenitor colony formation during the course of the disease was characterized. A detectable difference at the level of lymphocyte proliferation, apoptosis, and the relative amount of BFU-E, CFU-GM and CFU-GEMM cell colonies was revealed between healthy young mice and animals spontaneously developing pronounced symptoms of the autoimmune disorder. The quantity of BFU-E and CFU-GEMM colonies was remarkably increases in aged MRL/MpJ-lpr mice even before clinical manifestation of the disease (proteinuria). An elevated number of CFU-GEMM was accompanied by a striking increase in their size. The study of hematopoietic disturbances in autoimmune MRL/MpJ-lpr mice may be very useful for understanding the mechanism of the autoimmune disease development and searching for new strategies of the correction of the autoimmune disorder.
authors.affiliation: Institute of Clinical Immunology, Siberian Branch of RAMS, Yadrinzevskaya str. 14, Novosibirsk 630099, Russia.
authors.forename: Ludmila B
authors.initials: LB
authors.lastname: Toporkova
authors.forename: Viktoriya V
authors.initials: VV
authors.lastname: Dubrovskaya
authors.forename: Ludmila V
authors.initials: LV
authors.lastname: Sakhno
authors.forename: Marina A
authors.initials: MA
authors.lastname: Tikhonova
authors.forename: Elena R
authors.initials: ER
authors.lastname: Chernykh
authors.forename: Valentina N
authors.initials: VN
authors.lastname: Buneva
authors.forename: Georgy A
authors.initials: GA
authors.lastname: Nevinsky
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Kozlov
authors.forename: Irina A
authors.initials: IA
authors.lastname: Orlovskaya
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000276:immunology; D001327:Autoimmune Diseases / Q000209:etiology* / Q000276:immunology* / Q000473:pathology; D002455:Cell Division / Q000276:immunology; D002460:Cell Line; D006412:Hematopoietic Stem Cells / Q000276:immunology* / Q000473:pathology; D066298:In Vitro Techniques; D008198:Lymph Nodes / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D019463:Mice, Inbred MRL lpr; D011507:Proteinuria / Q000235:genetics / Q000378:metabolism