title: Nutritional evaluation of pea (Pisum sativum L.) protein diets after mild hydrothermal treatment and with and without added phytase.
abstract: The effect of mild hydrothermal treatment and the addition of phytase under optimal conditions (pH 5.5, 37 degrees C) on the nutritive utilization of the protein of pea (Pisum sativum L.) flour was studied in growing rats by examining the chemical and biological balance. Mild hydrothermal treatment produced reductions of 83, 78, and 72%, respectively, in the levels of alpha-galactosides, phytic acid, and trypsin inhibitors and also produced a significant increase in the digestive utilization of protein. The additional fall in the levels of phytic acid caused by the addition of phytase did not lead to a subsequent improvement in the digestive utilization of protein. The mild hydrothermal treatment of pea flour produced a significant increase in the metabolic utilization of protein and carbohydrates, which was reflected in the protein efficiency ratio and food transformation growth indices. These effects were not observed in the phytase-supplemented pea diet.
authors.affiliation: Departamento de Fisiología, Instituto de Nutrición, Universidad de Granada, Campus Universitario de Cartuja s/n, Granada 18071, Spain. paranda@ugr.es
authors.forename: Gloria
authors.initials: G
authors.lastname: Urbano
authors.forename: Pilar
authors.initials: P
authors.lastname: Aranda
authors.forename: Elena
authors.initials: E
authors.lastname: Gómez-Villalva
authors.forename: Sławomir
authors.initials: S
authors.lastname: Frejnagel
authors.forename: Jesus M
authors.initials: JM
authors.lastname: Porres
authors.forename: Juana
authors.initials: J
authors.lastname: Frías
authors.forename: Concepción
authors.initials: C
authors.lastname: Vidal-Valverde
authors.forename: María
authors.initials: M
authors.lastname: López-Jurado
mesh_terms: D010832:6-Phytase / Q000008:administration & dosage*; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D004044:Dietary Proteins / Q000008:administration & dosage / Q000032:analysis / Q000378:metabolism*; D004063:Digestion; D005260:Female; D005511:Food Handling; D005697:Galactosides / Q000032:analysis; D006358:Hot Temperature; D008099:Liver / Q000737:chemistry; D008297:Male; D018482:Muscle, Skeletal / Q000737:chemistry; D009584:Nitrogen / Q000032:analysis; D018532:Pisum sativum / Q000737:chemistry*; D010833:Phytic Acid / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D014361:Trypsin Inhibitors / Q000032:analysis; D014867:Water / Q000032:analysis
chemical_list: D004044:Dietary Proteins; D005697:Galactosides; D014361:Trypsin Inhibitors; D014867:Water; D010833:Phytic Acid; D010832:6-Phytase; D009584:Nitrogen