Antimetastatic efficacy of oral 5-FU imaged by green fluorescent protein in real time.
The effectiveness of oral 5-FU in suppressing liver metastasis was assessed in a highly-metastatic mouse model. Doses of 20 and 25 mg/kg oral 5-FU significantly suppressed primary and metastatic tumor growth (p = 0.012). These inhibitory effects were more dramatic in suppressing liver metastasis (p = 0.0). The efficacy of 5-FU was visualized by whole-body fluorescence imaging of the green fluorescent protein-expressing tumor and its subsequent metastases. Toxicity was observed only in the 30 mg/kg dose. Furthermore, we showed that the non-toxic doses of 5-FU significantly prolonged survival in these animals. These data suggest the important clinical potential of oral 5-FU.
Wang
Jin-Wei
JW
Anticancer, Inc., 7917 Ostrow Srteat, San Diego, CA 92111, USA.
Yang
Meng
M
Wang
Xiaoen
X
Sun
Fang-Xian
FX
Li
Xiao-Ming
XM
Yagi
Shigeo
S
Hoffman
Robert M
RM
D000284:Administration, Oral; D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005472:Fluorouracil / Q000494:pharmacology*; D049452:Green Fluorescent Proteins; D006801:Humans; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy* / Q000378:metabolism / Q000556:secondary*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008819:Mice, Nude; D023041:Xenograft Model Antitumor Assays
D000964:Antimetabolites, Antineoplastic; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins; D005472:Fluorouracil