title: Identification of a truncated Tn1721-like transposon located on a small plasmid of Escherichia coli isolated from Varanus indicus.
abstract: The 9.1 kb plasmid pDEWT1 was isolated from an Escherichia coli strain obtained from the faeces of a free-living lizard (Varanus indicus) in Indonesia. This plasmid mediated tetracycline resistance via a tet gene of hybridization class A. Molecular analysis of a 7755 bp segment of plasmid pDEWT1 including the tetR-tet(A) region and its flanking areas suggested that pDEWT1 harboured a truncated copy of the tet(A)-carrying transposon Tn1721 in which the part responsible for chemotaxis and transposition functions was lost. Analysis of the sequences at the integration site revealed the presence of the 5-bp direct repeat TACTT. The sequences upstream and downstream of the integration site showed striking homology to sequences of a non-coding region detectable on a small cryptic plasmid from Yersinia enterocolitica.
authors.affiliation: Department of Biology, Faculty of Science and Mathematics, IUC Biotechnology, Bogor Agricultural University, Indonesia.
authors.forename: D E
authors.initials: DE
authors.lastname: Waturangi
authors.forename: S
authors.initials: S
authors.lastname: Schwarz
authors.forename: A
authors.initials: A
authors.lastname: Suwanto
authors.forename: C
authors.initials: C
authors.lastname: Kehrenberg
authors.forename: W
authors.initials: W
authors.lastname: Erdelen
mesh_terms: D000818:Animals; D001483:Base Sequence; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D008116:Lizards / Q000382:microbiology*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D013753:Tetracycline Resistance / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial