Thermodynamic characterization of ferric and ferrous haem binding to a designed four-alpha-helix protein.
The thermodynamics of ferric and ferrous haem affinity of a de novo designed four-alpha-helix bundle protein and the associated haem electrochemistry is described.
Reedy
Charles J
CJ
Columbia University, Department of Chemistry, 3000 Broadway MC 3121, New York, NY 10027, USA.
Kennedy
Michelle L
ML
Gibney
Brian R
BR
D004563:Electrochemistry; D005290:Ferric Compounds / Q000378:metabolism*; D005296:Ferrous Compounds / Q000378:metabolism*; D006418:Heme / Q000737:chemistry* / Q000378:metabolism*; D010084:Oxidation-Reduction; D011485:Protein Binding; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics; D017186:Titrimetry / Q000379:methods
D005290:Ferric Compounds; D005296:Ferrous Compounds; D011506:Proteins; D006418:Heme