Expression of recombinant XVAX2 peptide-104 of Xenopus laevis and preparation of the antibody.
An N-terminal polypeptide (XVAX2-P104) of the XVAX2 protein, which is involved in controlling the dorsoventral patterning of the retina in Xenopus laevis, was expressed and purified as a Histagged fusion protein (pHis-XVAX2-P104), and it was employed to generate an anti-XVAX2 antibody in New Zealand white rabbits. ELISA analysis shows that the titer of the antibody is as high as approximately 1:100,000. The antibody could specifically recognize the full-length XVAX2 protein in extracts of Xenopus embryos, as determined by Western Blotting.
Liu
Yang
Y
Lab of Visual Information Processing, Centre for Brain and Cognitive Sciences, Institute of Biophysics, The Chinese Academy of Sciences, 100101 Beijing, PR China.
Liu
Ying
Y
Liu
Wei
W
Lupo
Giuseppe
G
He
Rong-Qiao
RQ
Barsacchi
Giuseppina
G
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D015153:Blotting, Western; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D018398:Homeodomain Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D014981:Xenopus; D029867:Xenopus Proteins
D000906:Antibodies; D018398:Homeodomain Proteins; D029867:Xenopus Proteins; C492361:vax2 protein, Xenopus