title: Laminin-5 gamma2-chain and collagenase-2 (MMP-8) in human peri-implant sulcular fluid.
abstract: Laminin-5 (LN-5) is an important epithelial cell-derived structural and adhesive component in hemidesmosomes and basement membranes (BM). In peri-implant tissue, gingival BM underlies the junctional epithelium (JE) and reflects the peri-implant health. Matrix metalloproteinase-8 (MMP-8 or collagenase-2) is one of the key mediators of periodontal tissue destruction. Western immunoblotting with image analysis was used to quantitate the molecular forms of LN-5 gamma2-chain and MMP-8 in peri-implant sulcular fluid (PISF) from healthy and diseased implants. These observations were related to the recorded gingival (GI) and bone resorption (BR) indices of the studied sites. Altogether, 72 PISF samples from osseointegrated dental implants were examined. Significantly elevated levels of fragmented LN-5 gamma2-chain species (45 and 70 kDa) and MMP-8 immunoreactivities were observed in diseased PISF in relation to healthy PISF. The elevated levels of both LN-5 gamma2-chain 45 and 70 kDa fragments and MMP-8 in diseased PISF from peri-mucositis (BR = 0) and peri-implantitis (BR >/= 1) lesions strongly correlated with elevated GI. Low levels - almost comparable to those seen in healthy control PISF - were seen in PISF from peri-implantitis lesions (BR >/= 1) with no GI. Activation of 75 kDa neutrophil (PMN)-type proMMP-8 to 10 kDa lower-molecular-size active forms was especially detected in PISF from peri-implantitis with elevated GI. These cross-sectional findings indicate that elevated MMP-8 and LN-5 gamma2-chain fragment levels in PISF can reflect the active phase of the inflammatory peri-implant disease. Longitudinal studies are required to assess their use, either alone or in combination as molecular biochemical PISF markers, to predict the risk of progression of peri-implantitis, as well as to monitor the impact of treatment of the disease.
authors.affiliation: Department of Oral and Maxillofacial Diseases, Helsinki University Central Hospital (HUCH), Institute of Dentistry, University of Helsinki, Helsinki, Finland. Marjo.Kivela@Helsinki.fi
authors.forename: Marjo J
authors.initials: MJ
authors.lastname: Kivelä-Rajamäki
authors.forename: Olli P
authors.initials: OP
authors.lastname: Teronen
authors.forename: Päivi
authors.initials: P
authors.lastname: Maisi
authors.forename: Ville
authors.initials: V
authors.lastname: Husa
authors.forename: Taina I
authors.initials: TI
authors.lastname: Tervahartiala
authors.forename: Emma M
authors.initials: EM
authors.lastname: Pirilä
authors.forename: Tuula A
authors.initials: TA
authors.lastname: Salo
authors.forename: Liisa
authors.initials: L
authors.lastname: Mellanen
authors.forename: Timo A
authors.initials: TA
authors.lastname: Sorsa
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016301:Alveolar Bone Loss / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis; D015153:Blotting, Western; D003430:Cross-Sectional Studies; D015921:Dental Implants; D018450:Disease Progression; D005260:Female; D005500:Follow-Up Studies; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D005891:Gingivitis / Q000378:metabolism; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007797:Laminin / Q000032:analysis*; D008297:Male; D020784:Matrix Metalloproteinase 8 / Q000032:analysis*; D008875:Middle Aged; D016348:Osseointegration; D010512:Periodontal Index; D010518:Periodontitis / Q000378:metabolism; D018709:Statistics, Nonparametric
chemical_list: D015415:Biomarkers; D015921:Dental Implants; C400567:LAMC2 protein, human; D007797:Laminin; D020784:Matrix Metalloproteinase 8