title: [Establishment of a human cervical carcinoma cell line HCC-0214 and its biological characteristics].
abstract: OBJECTIVE
  To establish a strain of human cervical carcinoma cell line and to provide a cervical carcinoma animal model.
  
  
  METHODS
  The cervical carcinoma specimens incised aseptically were cultured in vitro by tissue culture methods, giving a tumor cell growth curve. Morphology of the cells was observed, with cell cycling analysis and chromosome analysis performed. The tumor markers (ER, PR, Keratine, PCNA) expressions of the cell line were detected by immuno-cytochemical technique.
  
  
  RESULTS
  A human cervical carcinoma cell line HCC-0214 (H) has been obtained by in vitro tissue culture methods. The cells have been maintained for 16 months through 131 passages, showing a stable growth with a population doubling time of 35.48 h and a tendency to pile up without contact inhibition. The ultrastructure showed typical desmosomes and numerous tonofilaments. Chromosome analysis revealed the number of chromosomes per cell varied from 35-156 with a stem-line number of 58-80 (64.8%). The morphology of chromosomes showed human tumor cell structure. The tumor markers (ER, PR, Keratine, PCNA) of the cells showed a high expression. The DNA index was 1.931 by flow cytometry (FCM). The histopathology of the transplanted tumors in nude mice was the same as the original tumor, though with none successful by serum culture.
  
  
  CONCLUSIONS
  A human cervical carcinoma cell line HCC-0214 established by tissue culture is identical to the primary cancer cell in biological characters. After the cells have been passaged for more than 16 months continually, their characteristics are still retained. Therefore, HCC-0214 may be used as a stable cell line.
authors.affiliation: Department of Pathology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Hai-long
authors.initials: HL
authors.lastname: Dong
authors.forename: Yan-fang
authors.initials: YF
authors.lastname: Sui
authors.forename: Ping
authors.initials: P
authors.lastname: Qu
authors.forename: Zeng-shan
authors.initials: ZS
authors.lastname: Li
authors.forename: Shao-ying
authors.initials: SY
authors.lastname: Lu
authors.forename: Su-Zhen
authors.initials: SZ
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Ye
authors.forename: Guang-sheng
authors.initials: GS
authors.lastname: Chen
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division / Q000502:physiology; D005260:Female; D006801:Humans; D014407:Tumor Cells, Cultured / Q000502:physiology; D002583:Uterine Cervical Neoplasms / Q000473:pathology*