title: Ultrastructure, encystment and cyst wall composition of the resting cyst of the peritrich ciliate Opisthonecta henneguyi.
abstract: The cyst wall of Opisthonecta henneguyi has been studied ultrastructurally and cytochemically by light and electron microscopy, as well as by chemical and electrophoretic analyses, to examine the structure of the cyst wall and its composition. The cyst wall consists of four morphologically distinct layers. The ectocyst is a thin dense layer. The mesocyst is the thickest layer and is composed of a compact material. The endocyst is a thin layer like the ectocyst, but less dense. The granular layer varies in thickness and is composed of a granular material. In the resting cyst, kinetosomes of both oral apparatus and trochal band as well as the myoneme system are maintained, and only cilia are resorbed. The sugars present in the cyst wall are predominantly N-acetylglucosamine (90%) and glucose (10%). The mesocyst is composed of chitin, and the endocyst includes glycoproteins and acid mucopolysaccharides. During secretion of the cyst wall, the endocyst and granular layer are secreted from precursors synthesized "de novo". No cytoplasmic precursors of ectocyst and mesocyst have been detected.
authors.affiliation: Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla 41012 Sevilla, Spain. pcalvo@us.es
authors.forename: Purificación
authors.initials: P
authors.lastname: Calvo
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Fernandez-Aliseda
authors.forename: Jose
authors.initials: J
authors.lastname: Garrido
authors.forename: Antonio
authors.initials: A
authors.lastname: Torres
mesh_terms: D000818:Animals; D002473:Cell Wall / Q000737:chemistry / Q000648:ultrastructure; D002686:Chitin / Q000032:analysis / Q000737:chemistry; D037102:Lectins / Q000378:metabolism; D016802:Oligohymenophorea / Q000737:chemistry / Q000254:growth & development* / Q000648:ultrastructure*; D015800:Protozoan Proteins / Q000032:analysis
chemical_list: D037102:Lectins; D015800:Protozoan Proteins; D002686:Chitin