A story of chelatase evolution: identification and characterization of a small 13-15-kDa "ancestral" cobaltochelatase (CbiXS) in the archaea.
The cobaltochelatase required for the synthesis of vitamin B12 (cobalamin) in the archaeal kingdom has been identified as CbiX through similarity searching with the CbiX from Bacillus megaterium. However, the CbiX proteins in the archaea are much shorter than the CbiX proteins found in eubacteria, typically containing less than half the number of amino acids in their primary structure. For this reason the shorter CbiX proteins have been termed CbiXS and the longer versions CbiXL. The CbiXS proteins from Methanosarcina barkeri and Methanobacter thermoautotrophicum were overproduced in Escherichia coli as recombinant proteins and characterized. Through complementation studies of a defined chelatase-deficient strain of E. coli and by direct in vitro assays the function of CbiXS as a sirohydrochlorin cobaltochelatase has been demonstrated. On the basis of sequence alignments and conserved active site residues we suggest that CbiXS may represent a primordial chelatase, giving rise to larger chelatases such as CbiXL, SirB, CbiK, and HemH through gene duplication and subsequent variation and selection. A classification scheme for chelatases is proposed.
Brindley
Amanda A
AA
School of Biological Sciences, Queen Mary, University of London, Mile End Road, London E1 4NS, United Kingdom.
Raux
Evelyne
E
Leech
Helen K
HK
Schubert
Heidi L
HL
Warren
Martin J
MJ
D000595:Amino Acid Sequence; D001105:Archaea / Q000201:enzymology*; D001426:Bacterial Proteins; D002942:Circular Dichroism; D017124:Conserved Sequence; D019143:Evolution, Molecular; D008190:Lyases / Q000737:chemistry / Q000235:genetics*; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011487:Protein Conformation; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013056:Spectrophotometry, Ultraviolet
D001426:Bacterial Proteins; D008190:Lyases; C033211:cobaltochelatase