title: The involvement of cell cycle checkpoint-mutations in the mutagenesis induced in Drosophila by a longer wavelength light band of solar UV.
abstract: Solar ultraviolet radiation is considered to be injurious rather than necessary for most organisms living on the earth. It is reported that the risk of skin cancer in humans increases by the depletion of the ozone layer. We have examined the genotoxicity of solar ultraviolet, especially the longer wavelength light, using Drosophila. Recently, we have demonstrated that light of wavelength up to 340 nm is mutagenic on Drosophila larvae. Using an excision repair-deficient Drosophila strain (mus201), we have obtained results suggesting that the lesion caused in larvae by the 320 nm-light irradiation may be similar to the damage induced by irradiation at 310 nm, and that light of 330 and 340 nm may induce damage different from that induced by 310 and 320 nm-light. To examine the difference in DNA damage induced by light of a particular wavelength, we performed monochromatic irradiation on larvae of two Drosophila strains; one excision repair-deficient (mei-9) and another postreplication repair-deficient (mei-41). 310 and 320 nm-light was more mutagenic in the mei-9 strain than in mei-41, whereas 330 and 340 nm-light was more mutagenic in mei-41 than in mei-9. It is demonstrated that the mei-41 gene is a homologue of the human atm gene which is responsible for a cell cycle checkpoint. This result suggests that 310-320 nm-light induces DNA damage that is subject to nucleotide excision repair (NER) and that 330-360 nm-light causes damage to be recognized by the cell cycle checkpoint but it is not repairable by NER.
authors.affiliation: Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama 700-8530, Japan. isaka@pheasant.pharm.okayama-u.ac.jp
authors.forename: Megumi
authors.initials: M
authors.lastname: Toyoshima
authors.forename: Syogo
authors.initials: S
authors.lastname: Takinami
authors.forename: Kotaro
authors.initials: K
authors.lastname: Hieda
authors.forename: Yoshiya
authors.initials: Y
authors.lastname: Fursawa
authors.forename: Tomoe
authors.initials: T
authors.lastname: Negishi
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000235:genetics / Q000528:radiation effects*; D004260:DNA Repair; D004330:Drosophila / Q000166:cytology / Q000235:genetics / Q000528:radiation effects*; D016296:Mutagenesis; D009154:Mutation; D013472:Sunlight