title: [Nucleotide sequence analysis of new genotype of hepatitis G virus in population at high risk for HCV infection in Guangxi].
abstract: OBJECTIVE
  To examine the prevalence and the sequence of the genes of new genotypes of hepatitis G virus (HGV) in Guangxi, China.
  
  
  METHODS
  Serum samples were collected from 85 intravenous drug abusers (IVDAs), 80 patients with liver diseases (PLDs) and 50 blood donors (BDs). All sera (n=215) were tested by using EIA for HBsAg, anti-HCV and anti-HIV, and by using nested PCR for HGV RNA. In 62 subjects positive for HGV, HGV RNA was sequenced, and a phylogenetic tree was constructed for analyzing genotypes of HGV.
  
  
  RESULTS
  HGV RNA was detected in 85 of 215 serum samples (39.53%). The positivity rates for HBsAg, anti-HCV and anti-HIV were 39.07%, 42.79% and 0, respectively. First, 11 nucleotide sequences were determined and the isolates were grouped into three clusters with HGV. 5 of 11 HGV isolates clustered in a distinct phylogenetic branch (genotype Asia) which was different from the described GBV-C and HGV sequences, suggesting the presence of a new genotype of HGV in this locality. Second, 51 nucleotide sequences were determined and analyzed for their genotypes of HGV, and showed genotype GBV-C (3.23%), genotype HGV 30-65% and new genotype (genotype Asia) 64.51%, respectively.
  
  
  CONCLUSIONS
  There were subgenotypes in 3 genotypes of HGV; The predominant genotypes of HGV were genotype Asia and genotype HGV among IVDAs, PLDs, and BDs patients in Guangxi, China.
authors.affiliation: Guangxi Institute of Occupational Health, Nanning 530021, China.
authors.forename: Xianmin
authors.initials: X
authors.lastname: Ge
authors.forename: Danya
authors.initials: D
authors.lastname: Li
authors.forename: Rongrong
authors.initials: R
authors.lastname: Wu
authors.forename: Guoyong
authors.initials: G
authors.lastname: Huang
authors.forename: Haidong
authors.initials: H
authors.lastname: Pan
authors.forename: Kun
authors.initials: K
authors.lastname: Cao
authors.forename: Pingchuan
authors.initials: P
authors.lastname: Li
authors.forename: Shusheng
authors.initials: S
authors.lastname: Wang
authors.forename: Mizokami
authors.initials: M
authors.lastname: Masashi
mesh_terms: D000328:Adult; D001782:Blood Donors; D002681:China / Q000453:epidemiology; D005260:Female; D029407:GB virus C / Q000235:genetics* / Q000302:isolation & purification; D005838:Genotype; D006526:Hepatitis C / Q000453:epidemiology; D006801:Humans; D008107:Liver Diseases / Q000821:virology*; D008297:Male; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000235:genetics*; D017423:Sequence Analysis, RNA; D015819:Substance Abuse, Intravenous / Q000821:virology*
chemical_list: D012367:RNA, Viral