[Molecular cloning of human sperm surface protein P34H gene and semi-quantitative analysis of its expression in testis and epididymidis].
OBJECTIVE
To identify, clone and sequence human sperm protein P34H gene and perform semi-quantitative analysis of its expression in testis and epididymidis.


METHODS
Total RNA was prepared from human epididymidis tissue. cDNA fragment encoding human P34H was amplified by RT-PCR using specific primers, and then was cloned into pGEM-T vector. Inserted P34H gene was sequenced by ABI 377 DNA Sequencer. Meanwhile, semi-quantitative analysis of P34H expression in testis and caput, corpus, cauda epididymidis was done by RT-PCR based on beta-actin as an inner control.


RESULTS
Human sperm protein P34H gene was successfully cloned and its cDNA sequence was submitted to GenBank(Accession No. AF515625).


CONCLUSIONS
The cloning of P34H gene may be useful for the further basic and clinical studies on P34H.
Xia
Xin-Yi
XY
College of Life Science, Nanjing Normal University, Nanjing, Jiangsu 210097, China.
Xu
Xiao-Feng
XF
Gao
Yun
Y
Huang
Yu-Feng
YF
D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis; D004822:Epididymis / Q000378:metabolism*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013401:Sugar Alcohol Dehydrogenases; D013737:Testis / Q000378:metabolism*
D018076:DNA, Complementary; D011506:Proteins; D013401:Sugar Alcohol Dehydrogenases; C023300:L-xylulose reductase