title: N-terminus end of rat prostate transglutaminase is responsible for its catalytic activity and GTP binding.
abstract: Rat prostate transglutaminase is characterized by a high degree of complexity. In fact, as previously demonstrated, it is highly glycosilated and possesses a lipid anchor which is retained during enzyme apocrine secretion. In order to assess the importance of such modifications upon enzyme functionality, full length rat prostate transglutaminase cDNA has been synthesized by RT-PCR and stably expressed in MDCK cells. The recombinant form has been partially purified by GTP-affinity chromatography, a technique which has been used to purify the enzyme produced from rat prostate secretion. The recombinant protein is endowed with enzymatic activity even though, as we have demonstrated by immunological studies, it lacks post-translational modifications which occur in the prostate enzyme. Moreover, we have demonstrated that a deletion mutant, which gives rise to a protein lacking 103 amino acid residues at the N-terminus end, loses enzymatic activity and the capability of binding GTP. This study shows that, while post-translational modifications are not essential for enzymatic activity, the N-terminus end is responsible for both transglutaminase functionality and GTP-binding.
authors.affiliation: Department of Food Science, University of Naples "Federico II", Parco Gussone Portici, 80055 Naples, Italy. loremari@unina.it
authors.forename: Loredana
authors.initials: L
authors.lastname: Mariniello
authors.forename: Carla
authors.initials: C
authors.lastname: Esposito
authors.forename: Ivana
authors.initials: I
authors.lastname: Caputo
authors.forename: Angela
authors.initials: A
authors.lastname: Sorrentino
authors.forename: Raffaele
authors.initials: R
authors.lastname: Porta
mesh_terms: D000818:Animals; D020134:Catalytic Domain; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000235:genetics; D004285:Dogs; D019204:GTP-Binding Proteins / Q000378:metabolism*; D015151:Immunoblotting; D008297:Male; D011467:Prostate / Q000201:enzymology*; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism*; D011503:Transglutaminases / Q000096:biosynthesis / Q000378:metabolism*
chemical_list: D018076:DNA, Complementary; D011994:Recombinant Proteins; D011503:Transglutaminases; D019204:GTP-Binding Proteins