Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis.
The complete genome sequence of Enterococcus faecalis V583, a vancomycin-resistant clinical isolate, revealed that more than a quarter of the genome consists of probable mobile or foreign DNA. One of the predicted mobile elements is a previously unknown vanB vancomycin-resistance conjugative transposon. Three plasmids were identified, including two pheromone-sensing conjugative plasmids, one encoding a previously undescribed pheromone inhibitor. The apparent propensity for the incorporation of mobile elements probably contributed to the rapid acquisition and dissemination of drug resistance in the enterococci.
Paulsen
I T
IT
Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA. ipaulsen@tigr.org
Banerjei
L
L
Myers
G S A
GS
Nelson
K E
KE
Seshadri
R
R
Read
T D
TD
Fouts
D E
DE
Eisen
J A
JA
Gill
S R
SR
Heidelberg
J F
JF
Tettelin
H
H
Dodson
R J
RJ
Umayam
L
L
Brinkac
L
L
Beanan
M
M
Daugherty
S
S
DeBoy
R T
RT
Durkin
S
S
Kolonay
J
J
Madupu
R
R
Nelson
W
W
Vamathevan
J
J
Tran
B
B
Upton
J
J
Hansen
T
T
Shetty
J
J
Khouri
H
H
Utterback
T
T
Radune
D
D
Ketchum
K A
KA
Dougherty
B A
BA
Fraser
C M
CM
D018829:Adhesins, Bacterial / Q000235:genetics; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics; D005075:Biological Evolution; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D002876:Chromosomes, Bacterial / Q000235:genetics; D003227:Conjugation, Genetic; D017124:Conserved Sequence; D004251:DNA Transposable Elements; D004064:Digestive System / Q000382:microbiology; D024901:Drug Resistance, Multiple, Bacterial; D013293:Enterococcus faecalis / Q000187:drug effects / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D022761:Gene Transfer, Horizontal; D016680:Genome, Bacterial; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology; D006801:Humans; D020071:Interspersed Repetitive Sequences; D008242:Lysogeny; D016366:Open Reading Frames; D018384:Oxidative Stress; D010957:Plasmids; D017422:Sequence Analysis, DNA; D026801:Synteny; D020713:Vancomycin Resistance / Q000235:genetics*; D014774:Virulence / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics
D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D002352:Carrier Proteins; D004251:DNA Transposable Elements; D037521:Virulence Factors