title: Preconditioning of donors with interleukin-10 reduces hepatic ischemia-reperfusion injury after liver transplantation in pigs.
abstract: BACKGROUND
  Graft ischemia-reperfusion injury (IRI) resulting from postreperfusion inflammatory reaction remains a major cause of complications after liver transplantation. In this article, the authors investigated the effect of anti-inflammatory cytokine interleukin (IL)-10 on IRI, in a preclinical model of liver transplantation in pigs.
  
  
  METHODS
  Donor pigs received IL-10 or saline at the start of liver graft harvesting. After 5 hr of cold ischemia, liver grafts were transplanted into untreated recipient pigs. IRI severity was measured in recipients by transaminase release and by cellular infiltration and necrosis on liver biopsy specimens.
  
  
  RESULTS
  Donor IL-10 administration attenuated IRI, as indicated by significant reduction of mean peak of transaminase in recipients of grafts from IL-10-treated donors. In contrast, no significant differences in cell infiltration or amount of necrosis were observed on liver biopsy specimens between groups.
  
  
  CONCLUSIONS
  Donor preconditioning with IL-10 may constitute an interesting pharmacologic approach to reduce IRI severity after liver transplantation.
authors.affiliation: Medicosurgical Department of Hepatogastroenterology, Hôpital Erasme, Université Libre de Bruxelles, Brussels, Belgium. vdonckie@ulb.ac.be.
authors.forename: Vincent
authors.initials: V
authors.lastname: Donckier
authors.forename: Patricia
authors.initials: P
authors.lastname: Loi
authors.forename: Jean
authors.initials: J
authors.lastname: Closset
authors.forename: Nathalie
authors.initials: N
authors.lastname: Nagy
authors.forename: Eric
authors.initials: E
authors.lastname: Quertinmont
authors.forename: Olivier
authors.initials: O
authors.lastname: Le Moine
authors.forename: Jacques
authors.initials: J
authors.lastname: Devière
authors.forename: Michel
authors.initials: M
authors.lastname: Goldman
authors.forename: Michel
authors.initials: M
authors.lastname: Gelin
authors.forename: Pierre
authors.initials: P
authors.lastname: Gianello
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D006085:Graft Survival / Q000187:drug effects; D016753:Interleukin-10 / Q000097:blood / Q000494:pharmacology*; D019194:Ischemic Preconditioning; D016031:Liver Transplantation; D015427:Reperfusion Injury / Q000517:prevention & control*; D013552:Swine; D014019:Tissue Donors
chemical_list: D016753:Interleukin-10; D000410:Alanine Transaminase