Differential oxidative damage and expression of stress defence regulons in culturable and non-culturable Escherichia coli cells.
Potentially pathogenic bacteria, such as Escherichia coli and Vibrio cholerae, become non-culturable during stasis. The analysis of such cells has been hampered by difficulties in studying bacterial population heterogeneity. Using in situ detection of protein oxidation in single E. coli cells, and using a density-gradient centrifugation technique to separate culturable and non-culturable cells, we show that the proteins in non-culturable cells show increased and irreversible oxidative damage, which affects various bacterial compartments and proteins. The levels of expression of specific stress regulons are higher in non-culturable cells, confirming increased defects relating to oxidative damage and the occurrence of aberrant, such as by amino-acid misincorporation, proteins. Our data suggest that non-culturable cells are produced due to stochastic deterioration, rather than an adaptive programme, and pinpoint oxidation management as the 'Achilles heel' of these cells.
Desnues
Benoît
B
Laboratoire de Microbiologie Marine, CNRS-INSU-UMR 6117, Université de la Méditerranée, Marseille, France.
Cuny
Caroline
C
Grégori
Gérald
G
Dukan
Sam
S
Aguilaniu
Hugo
H
Nyström
Thomas
T
D000818:Animals; D000911:Antibodies, Monoclonal; D000975:Antioxidants / Q000378:metabolism; D002455:Cell Division; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics / Q000254:growth & development* / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D018384:Oxidative Stress / Q000502:physiology*
D000911:Antibodies, Monoclonal; D000975:Antioxidants; D029968:Escherichia coli Proteins