Structure determination of a small protein through a 23-dimensional molecular-replacement search.
The crystal structure of a 4-alpha-helical bundle protein has been determined by the application of a 23-dimensional molecular-replacement search performed using a stochastic method. The search model for the calculation was a 26-residue-long polyalanine helix amounting to less than 13% of the total number of atoms in the asymmetric unit of the target crystal structure. The crystal structure determination procedure is presented in detail, with emphasis on the molecular-replacement calculations.
Glykos
Nicholas M
NM
IMBB, FORTH, Heraklion, Crete, Greece. glykos@imbb.forth.gr
Kokkinidis
Michael
M
D001426:Bacterial Proteins / Q000737:chemistry*; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D015233:Models, Statistical; D009154:Mutation; D010455:Peptides / Q000737:chemistry; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry*; D016601:RNA-Binding Proteins / Q000737:chemistry*; D012984:Software; D013269:Stochastic Processes
D001426:Bacterial Proteins; D010455:Peptides; D011506:Proteins; D016601:RNA-Binding Proteins; C054243:Rop protein, ColE1 plasmid; C019529:polyalanine