title: C. elegans ORFeome version 1.1: experimental verification of the genome annotation and resource for proteome-scale protein expression.
abstract: To verify the genome annotation and to create a resource to functionally characterize the proteome, we attempted to Gateway-clone all predicted protein-encoding open reading frames (ORFs), or the 'ORFeome,' of Caenorhabditis elegans. We successfully cloned approximately 12,000 ORFs (ORFeome 1.1), of which roughly 4,000 correspond to genes that are untouched by any cDNA or expressed-sequence tag (EST). More than 50% of predicted genes needed corrections in their intron-exon structures. Notably, approximately 11,000 C. elegans proteins can now be expressed under many conditions and characterized using various high-throughput strategies, including large-scale interactome mapping. We suggest that similar ORFeome projects will be valuable for other organisms, including humans.
authors.affiliation: Dana-Farber Cancer Institute and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Reboul
authors.forename: Philippe
authors.initials: P
authors.lastname: Vaglio
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Rual
authors.forename: Philippe
authors.initials: P
authors.lastname: Lamesch
authors.forename: Monica
authors.initials: M
authors.lastname: Martinez
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Armstrong
authors.forename: Siming
authors.initials: S
authors.lastname: Li
authors.forename: Laurent
authors.initials: L
authors.lastname: Jacotot
authors.forename: Nicolas
authors.initials: N
authors.lastname: Bertin
authors.forename: Rekin's
authors.initials: R
authors.lastname: Janky
authors.forename: Troy
authors.initials: T
authors.lastname: Moore
authors.forename: James R
authors.initials: JR
authors.lastname: Hudson
authors.forename: James L
authors.initials: JL
authors.lastname: Hartley
authors.forename: Michael A
authors.initials: MA
authors.lastname: Brasch
authors.forename: Jean
authors.initials: J
authors.lastname: Vandenhaute
authors.forename: Simon
authors.initials: S
authors.lastname: Boulton
authors.forename: Gregory A
authors.initials: GA
authors.lastname: Endress
authors.forename: Sarah
authors.initials: S
authors.lastname: Jenna
authors.forename: Eric
authors.initials: E
authors.lastname: Chevet
authors.forename: Vasilis
authors.initials: V
authors.lastname: Papasotiropoulos
authors.forename: Peter P
authors.initials: PP
authors.lastname: Tolias
authors.forename: Jason
authors.initials: J
authors.lastname: Ptacek
authors.forename: Mike
authors.initials: M
authors.lastname: Snyder
authors.forename: Raymond
authors.initials: R
authors.lastname: Huang
authors.forename: Mark R
authors.initials: MR
authors.lastname: Chance
authors.forename: Hongmei
authors.initials: H
authors.lastname: Lee
authors.forename: Lynn
authors.initials: L
authors.lastname: Doucette-Stamm
authors.forename: David E
authors.initials: DE
authors.lastname: Hill
authors.forename: Marc
authors.initials: M
authors.lastname: Vidal
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018509:DNA, Helminth / Q000235:genetics; D030541:Databases, Genetic; D005091:Exons; D020224:Expressed Sequence Tags; D015870:Gene Expression; D017238:Genes, Helminth; D016678:Genome; D023281:Genomics; D015801:Helminth Proteins / Q000235:genetics; D006801:Humans; D007438:Introns; D016366:Open Reading Frames; D020543:Proteome; D040901:Proteomics
chemical_list: D018076:DNA, Complementary; D018509:DNA, Helminth; D015801:Helminth Proteins; D020543:Proteome