The apoptosis-associated protein BNIPL interacts with two cell proliferation-related proteins, MIF and GFER.
Bcl-2/adenovirus E1B 19 kDa interacting protein 2-like, BNIP-2-like (BNIPL) is a recently cloned and characterized apoptosis-associated protein that shares 72% homology with BNIP-2. It is highly expressed in human placenta and lung. A yeast two-hybrid system was used to obtain two BNIPL-interacting proteins, MIF (macrophage migration inhibitory factor) and GFER (growth factor erv1 (Saccharomyces cerevisiae)-like). The interactions were confirmed by glutathione S-transferase pull-down assay in vitro and co-immunoprecipitation assay in vivo. Colony formation assay and cell proliferation test suggest that overexpression of BNIPL could inhibit the growth of BEL-7402 cells. These findings suggest that BNIPL may physically bind to cell proliferation-related proteins, MIF and GFER.
Shen
Li
L
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, PR China.
Hu
Jian
J
Lu
Hong
H
Wu
Ming
M
Qin
Wenxin
W
Wan
Dafang
D
Li
Yu-Yang
YY
Gu
Jianren
J
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000378:metabolism*; D006801:Humans; D011233:Precipitin Tests; D011485:Protein Binding; D020798:Two-Hybrid System Techniques
D048868:Adaptor Proteins, Signal Transducing; C450834:BNIPL protein, human; D002352:Carrier Proteins; D018797:Cell Cycle Proteins