[Cloning of human monoclonal Fab fragments against HIV-1 gp120 peptide binding chemokine receptor from phage Fab antibody library].
OBJECTIVE
To screen human monoclonal Fab fragments against HIV-1 gp120 peptide binding chemokine receptor.


METHODS
A synthesized polypeptide containing 23 amino acid residues of the gp120 antigen epitope binding chemokine receptor was coated as the solid-phase antigen. After biopanning from the HIV-1 phage Fab antibody library, the acquired positive clones were tested and sequenced.


RESULTS
One clone of human phage Fab monoclonal antibody against HIV-1 gp120 polypeptide was acquired. It has high affinity, specificity and inhibition rate and it belongs to IgG I subclass and kappa type. Its Vh H and V kappa were derived from Vh III and V kappa III.


CONCLUSIONS
The human phage Fab fragment against HIV-1 gp120 antigen site binding chemokine receptor was acquired.
Shen
Honghui
H
Department of Virology, The 302nd Hospital of PLA, Beijing 100039, China.
Mao
Panyong
P
Hong
Shiwen
S
Hou
Jun
J
Yang
Jianyang
J
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000235:genetics*; D000914:Antibodies, Viral / Q000235:genetics*; D001435:Bacteriophages / Q000235:genetics; D003001:Cloning, Molecular; D015699:HIV Envelope Protein gp120; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000235:genetics*; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D019151:Peptide Library; D019707:Receptors, Chemokine / Q000378:metabolism; D015728:Receptors, HIV / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D015699:HIV Envelope Protein gp120; D007140:Immunoglobulin Fab Fragments; D019151:Peptide Library; D019707:Receptors, Chemokine; D015728:Receptors, HIV