Prodigiosin induces apoptosis of B and T cells from B-cell chronic lymphocytic leukemia.
We have previously reported that prodigiosin (2-methyl-3-pentyl-6-methoxyprodigiosene) induces apoptosis in human hematopoietic cancer cell lines with no marked toxicity in nonmalignant cell lines. In this study, we demonstrate that prodigiosin induces apoptosis of B-cell chronic lymphocytic leukemia (B-CLL) cells (n=32 patients). The dose-response for the cytotoxic effect of prodigiosin was analyzed in cells from 12 patients showing an IC(50) of 116+/-25 nM. Prodigiosin induced apoptosis of B-CLL cells through caspase activation. We also analyzed the cytotoxic effect of prodigiosin in T cells from B-CLL samples and no differences were observed with respect to leukemia cells. This is the first report showing that prodigiosin induces apoptosis in human primary cancer cells.
Campàs
C
C
Department de Ciències Fisiològiques II, Unitat de Bioquímica, Facultat de Medicine, Universtitat de barcelona, Spain.
Dalmau
M
M
Montaner
B
B
Barragán
M
M
Bellosillo
B
B
Colomer
D
D
Pons
G
G
Pérez-Tomás
R
R
Gil
J
J
D017920:Antiporters / Q000187:drug effects; D017209:Apoptosis / Q000187:drug effects*; D001402:B-Lymphocytes / Q000737:chemistry / Q000187:drug effects* / Q000473:pathology; D020169:Caspases / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D017136:Ion Transport / Q000187:drug effects; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000378:metabolism / Q000473:pathology*; D009363:Neoplasm Proteins / Q000032:analysis / Q000378:metabolism; D014411:Neoplastic Stem Cells / Q000737:chemistry / Q000187:drug effects* / Q000473:pathology; D011353:Prodigiosin / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000737:chemistry / Q000187:drug effects* / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000187:drug effects; D016159:Tumor Suppressor Protein p53 / Q000032:analysis
D017920:Antiporters; D009363:Neoplasm Proteins; D016159:Tumor Suppressor Protein p53; C114260:hydrogen-chloride symporter; D020169:Caspases; D011353:Prodigiosin