Determination of 4-n-nonylphenol in water by enzyme immunoassay.
A range of monoclonal antibody-based competitive immunoassays in the format of microtitre plate ELISA and dipstick tests for quantitative and semi-quantitative detection of 4- n -nonylphenol in water was developed. A simple visual dipstick test was based on changing of spot colour from green to brown in the presence of 4- n -nonylphenol at concentrations within the range 10-100 ng mL(-1). Two different detection systems were used for quantitative immunoassay. Application of enhanced chemiluminescence (ECL) resulted in an increase of the sensitivity of ELISA when compared to conventional colorimetric detection. Thus a detection limit of 0.06 ng mL(-1 )of 4- n -nonylphenol was achieved with IC(50) 2.0 ng mL(-1). The tests developed were applied to natural and spiked water samples.
Samsonova
Jeanne V
JV
Department of Chemical Enzymology, Chemistry Faculty, M.V. Lomonosov Moscow State University, 119992, Moscow, Russia. jvs@enz.chem.msu.ru
Rubtsova
Maya Yu
MY
Franek
Milan
M
D004797:Enzyme-Linked Immunosorbent Assay; D007124:Immunoenzyme Techniques / Q000379:methods*; D009757:Nylons; D010636:Phenols / Q000032:analysis*; D012680:Sensitivity and Specificity; D014867:Water / Q000737:chemistry*
D009757:Nylons; D010636:Phenols; D014867:Water; C025256:nonylphenol