Using bifunctional polymers presenting vancomycin and fluorescein groups to direct anti-fluorescein antibodies to self-assembled monolayers presenting d-alanine-d-alanine groups.
This paper describes the synthesis of bifunctional polyacrylamides containing pendant vancomycin (Van) and fluorescein groups, and the use of these polymers to direct antibodies against fluorescein to self-assembled monolayers (SAMs) presenting d-alanine-d-alanine (dAdA) groups. These polymers bind biospecifically to these SAMs via interactions between the dAdA and Van groups and serve as a molecular bridge between the anti-fluorescein antibodies and the SAM. The binding events were characterized using surface plasmon resonance spectroscopy and fluorescence microscopy. The paper demonstrates that polyvalent, biospecific, noncovalent interactions between a polymer and a surface can be used to tailor the properties of the surface in molecular recognition. It also represents a first step toward the design of polymers that direct arbitrarily chosen antibodies to the surfaces of cells.
Metallo
Steven J
SJ
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA.
Kane
Ravi S
RS
Holmlin
R Erik
RE
Whitesides
George M
GM
D000180:Acrylic Resins / Q000138:chemical synthesis* / Q000737:chemistry; D000906:Antibodies / Q000737:chemistry* / Q000276:immunology; D004151:Dipeptides / Q000737:chemistry*; D005452:Fluoresceins / Q000737:chemistry*; D005455:Fluorescent Antibody Technique; D008856:Microscopy, Fluorescence; D020349:Surface Plasmon Resonance; D013499:Surface Properties; D014640:Vancomycin / Q000737:chemistry*; D000099166:Polyacrylamides
D000180:Acrylic Resins; D000906:Antibodies; D004151:Dipeptides; D005452:Fluoresceins; D014640:Vancomycin; D000099166:Polyacrylamides; C002956:alanylalanine