[Influence of endothelial cells on the proliferation of scar-derived fibroblast in hypertropic scar tissue].
OBJECTIVE
To investigate the influence of the endothelial cells on the scar-derived fibroblasts in the hypertropic scar tissue.


METHODS
The endothelial cell from the human umbilical vein was cultured and its supernatant was then collected. Thereafter, it was mixed with the pre-cultured scar-derived fibroblasts from the hypertropic scar tissue. The fibroblasts were analyzed with a flow cytometer in a MTT-assay method to evaluate the cell proliferation and its division cycle.


RESULTS
The endothelial cells were significantly increasing the scar-derived fibroblast proliferation (P < 0.05), compared with the control. The proportion of the fibroblasts in the S-stage of cell cycle was also quite high.


CONCLUSIONS
The endothelial cells may secrete some active products, which may enhance the proliferation of the scar-derived fibroblasts and stimulate the fibroblasts into the S-stage of cell generation cycle.
Zhang
Linxi
L
Fourth Military Medical University, Xi'an 710032, China.
Guo
Shuzhong
S
Xia
Wensen
W
Yang
Li
L
Wang
Zhen
Z
D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D017439:Cicatrix, Hypertrophic / Q000473:pathology*; D017077:Culture Media, Conditioned / Q000737:chemistry / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000737:chemistry* / Q000166:cytology; D005347:Fibroblasts / Q000187:drug effects* / Q000473:pathology; D006801:Humans
D017077:Culture Media, Conditioned