title: [Induction or inhibition of apoptosis by pseudorabies virus ea strain in suckling piglets].
abstract: To study induction or inhibition of apoptosis by pseudorabies virus (PrV) Ea strain in suckling piglets, Aujeszky s disease was replicated by artificially inoculating 15 day-old piglets with PrV-Ea strain. Various tissue sections, such as lymphoid tissues and nervous tissues were collected. Transmission electron microscopy, DNA fragmentation assay, and in situ terminal-deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) staining were carried to analyze apoptotic cells. It was shown that piglets infected with PrV-Ea strain took on typical clinical symptoms and apoptotic cells were found in lymphoid tissues but not in nerve tissues. The results indicated that the PrV infection caused apoptosis in a big number of lymphatic cells, thus leading to death of suckling piglets due to lowered immune function. It might play an important role in PrV s attack on immune system. A latent infection of pseudorabies virus was established in the neuronal tissue cells by inhibition of nervous cell apoptosis; this might be the way for PrV to establish latent infection, leading to sporadic recurrence of the disease.
authors.affiliation: Laboratory of Animal Virology, College of Animal Medical Science, Huazhong Agricultural University, Wuhan 430070, China. Hzauvet@public.wh.hb.cn
authors.forename: Xiang-Min
authors.initials: XM
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Qian
authors.forename: De-Shi
authors.initials: DS
authors.lastname: Shi
authors.forename: Mei-Lin
authors.initials: ML
authors.lastname: Jin
authors.forename: Sheng-Yin
authors.initials: SY
authors.lastname: Lan
authors.forename: Huan-Chun
authors.initials: HC
authors.lastname: Chen
mesh_terms: D000818:Animals; D000833:Animals, Suckling; D017209:Apoptosis; D004279:DNA, Viral / Q000235:genetics; D011558:Herpesvirus 1, Suid / Q000235:genetics / Q000254:growth & development*; D020287:In Situ Nick-End Labeling; D008221:Lymphoid Tissue / Q000473:pathology / Q000648:ultrastructure / Q000821:virology; D008854:Microscopy, Electron; D009420:Nervous System / Q000473:pathology / Q000648:ultrastructure / Q000821:virology; D016133:Polymerase Chain Reaction; D011557:Pseudorabies / Q000276:immunology / Q000473:pathology / Q000821:virology*; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000473:pathology* / Q000821:virology
chemical_list: D004279:DNA, Viral