title: Paradoxical early upregulation of intragraft Th1 cytokines is associated with graft acceptance following donor-specific blood transfusion.
abstract: Donor-specific blood transfusion (DSBT) promotes graft acceptance in certain experimental and clinical situations. However, the exact nature of the operating mechanisms, and in particular the role of Th1/Th2 cytokines, remains controversial. We recently described a fully mismatched rat combination [RA rat (RT1p) to PVG rat (RT1c)] in which a single pre-transplant (Tx) DSBT induces donor-specific tolerance following heart transplantation (Htx). In order to delineate better the role of Th1 and Th2 cytokines in the development of tolerance versus rejection, we studied the intragraft mRNA for Th1 and Th2 cytokines at different time points post-Tx in rejecting and DSBT-tolerized heart grafts. We performed reverse transcriptase-polymerase chain reaction (RT-PCR) to examine intragraft Th1/Th2 cytokines profile. In contrast to findings from previous data, we detected an early (post-Tx day 2) Th1 cytokine upregulation. Th1 downregulation/Th2 immune deviation was observed only at a later period (post-Tx day 30). Consistent with this Th1 early upregulation, a dense mononuclear cellular infiltrate was seen in tolerized grafts, which was equivalent to that seen in rejecting grafts. Despite in vivo unresponsiveness, peripheral lymphocyte derived from tolerant animals proliferated against donor-matched antigens to the same degree as against those of a third party until post-Tx day 30. Altogether, these observations (dense graft cellular infiltrate, early post-Tx Th1 cytokine production, early preserved cellular proliferative responses), suggest that the development of tolerance is preceded by an active Th1 cytokine-mediated immune response. The mechanisms by which an early Th1 response promotes tolerance need to be further studied.
authors.affiliation: Abdominal Transplant Department, University Hospital of Leuven, Herestraat 49, 3000, Leuven, Belgium.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Koshiba
authors.forename: Annapaula
authors.initials: A
authors.lastname: Giulietti
authors.forename: Boudewijn
authors.initials: B
authors.lastname: Van Damme
authors.forename: Lut
authors.initials: L
authors.lastname: Overbergh
authors.forename: Omer
authors.initials: O
authors.lastname: Rutgeerts
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kitade
authors.forename: Mark
authors.initials: M
authors.lastname: Waer
authors.forename: Chantal
authors.initials: C
authors.lastname: Mathieu
authors.forename: Jacques
authors.initials: J
authors.lastname: Pirenne
mesh_terms: D000818:Animals; D001483:Base Sequence; D001803:Blood Transfusion / Q000379:methods*; D016207:Cytokines / Q000235:genetics*; D017931:DNA Primers; D005786:Gene Expression Regulation / Q000276:immunology; D016027:Heart Transplantation / Q000276:immunology*; D007108:Immune Tolerance; D007165:Immunosuppression Therapy / Q000379:methods; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018417:Th1 Cells / Q000276:immunology*; D014019:Tissue Donors; D014158:Transcription, Genetic
chemical_list: D016207:Cytokines; D017931:DNA Primers