title: Dendritic cells under influence of mouse cytomegalovirus have a physiologic dual role: to initiate and to restrict T cell activation.
abstract: The aim of this study is to analyze the dynamics of the mouse cytomegalovirus (MCMV)-dendritic cell (DC) interaction. Immature and mature DCs derived from the mouse stem cell line factor-dependent cell Paterson mixed potential were infected with a recombinant MCMV expressing green fluorescent protein. Infection of immature DCs resulted in DC activation and virus production, both of which may contribute to viral dissemination. The infection of mature DCs was nonproductive and was restricted to immediate-early and early viral protein expression. During early stages of MCMV infection, mature DCs up-regulated major histocompatibility complex (MHC) and costimulatory molecules and activated autologous, but not allogeneic, naive T cells. At later times of MCMV infection, DCs prevented T cell activation by down-regulation of MHC and costimulatory molecules. Thus, DCs under the influence of MCMV have a physiologic dual role: to initiate and to restrict T cell activation. The lack of immunostimulation in allogeneic settings may explain the increased risk of MCMV morbidity after allogeneic transplantation.
authors.affiliation: Institut für Klinische Molekularbiologie und Tumorgenetik des GSF, Genzentrum, Ludwig-Maximilians-Universität München, Germany.
authors.forename: Sibylle
authors.initials: S
authors.lastname: Mathys
authors.forename: Timm
authors.initials: T
authors.lastname: Schroeder
authors.forename: Joachim
authors.initials: J
authors.lastname: Ellwart
authors.forename: Ulrich H
authors.initials: UH
authors.lastname: Koszinowski
authors.forename: Martin
authors.initials: M
authors.lastname: Messerle
authors.forename: Ursula
authors.initials: U
authors.lastname: Just
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D003713:Dendritic Cells / Q000276:immunology / Q000821:virology*; D049452:Green Fluorescent Proteins; D006566:Herpesviridae Infections / Q000276:immunology* / Q000821:virology; D008164:Luminescent Proteins / Q000235:genetics; D008213:Lymphocyte Activation; D008285:Major Histocompatibility Complex / Q000276:immunology; D051379:Mice; D018146:Muromegalovirus / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D011995:Recombination, Genetic; D013601:T-Lymphocytes / Q000276:immunology*; D015854:Up-Regulation; D014779:Virus Replication
chemical_list: D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins