Detection of hepatitis B virus DNA in sera from patients with chronic hepatitis B virus infection by DNA microarray method.
We have developed a sensitive and quantitative assay using a DNA microarray for the detection of hepatitis B virus (HBV) DNA in serum. Fluorescently labeled target cDNA prepared from cloned HBV DNA or serum HBV DNA was hybridized to capture DNA on a slide. A linear relationship was obtained between the intensities of the array spot and the amount of the cloned or serum HBV DNA, indicating the quantitative accuracy of this assay system. In addition, there was a significant correlation between the number of molecules of serum HBV DNA determined by the DNA microarray and that determined by a branched-DNA assay (n = 21, r = 0.89). Given these results, we conclude that the DNA microarray assay system may be useful as a diagnostic technique in the clinical laboratory.
Kawaguchi
Kazunori
K
Gastroenterology, Kanazawa University Graduate School of Medical Science, Takara-Machi 13-1, Kanazawa 920-8641, Japan.
Kaneko
Shuichi
S
Honda
Masao
M
Kawai
Hiroshi F
HF
Shirota
Yukihiro
Y
Kobayashi
Kenichi
K
D021121:Branched DNA Signal Amplification Assay; D004279:DNA, Viral / Q000097:blood*; D006515:Hepatitis B virus / Q000235:genetics / Q000302:isolation & purification*; D019694:Hepatitis B, Chronic / Q000821:virology*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012680:Sensitivity and Specificity
D004279:DNA, Viral