Purification, crystallization and preliminary X-ray analysis of the Enterococcus faecalis protein EF0377.
The EF0377 gene of Enterococcus faecalis was cloned and overexpressed in Escherichia coli. The protein has been purified and crystallized in three forms. Type III crystals belong to space group P2(1), with unit-cell parameters a = 72.11, b = 94.97, c = 80.77 A, beta = 111.93 degrees. There are four molecules per asymmetric unit and diffraction is observed to beyond 1.65 A under cryoconditions (100 K) using synchrotron radiation. An almost complete set of X-ray diffraction data was collected to 1.9 A from the native crystal.
Riboldi-Tunnicliffe
Alan
A
IBLS Infection and Immunity, University of Glasgow, Scotland.
Bent
Colin J
CJ
Isaacs
Neil W
NW
Mitchell
Timothy J
TJ
D017089:Ankyrin Repeat; D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D013293:Enterococcus faecalis / Q000737:chemistry*; D004926:Escherichia coli / Q000378:metabolism; D005798:Genes, Bacterial / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014961:X-Ray Diffraction
D001426:Bacterial Proteins; D002352:Carrier Proteins; C478744:EF0377 protein, Enterococcus faecalis