Enzymatic preparation of ginsenosides Rg2, Rh1, and F1 from protopanaxatriol-type ginseng saponin mixture.
During investigations on the hydrolysis of a protopanaxatriol-type saponin mixture by various glycoside hydrolases, it was found that two minor saponins, ginsenosides Rg 2 and Rh 1, were formed in high yields by crude beta-galactosidase from Aspergillus oryzae and crude lactase from Penicillium sp., respectively. Moreover, a crude preparation of naringinase from Penicillium decumbens readily hydrolyzed a protopanaxatriol-type saponin mixture to give an intestinal bacterial metabolite, ginsenoside F 1 as the main product. A crude preparation of hesperidinase from Penicillium sp. selectively hydrolyzed ginsenoside Re into ginsenoside Rg 1. This is the first report on the enzymatic preparation of minor saponins, ginsenosides Rg 2 and Rh 1, and of an intestinal bacterial metabolite, ginsenoside F 1, with a high efficiency from a protopanaxatriol-type saponin mixture.
Ko
Sung-Ryong
SR
Choi
Kang-Ju
KJ
Uchida
Kei
K
Suzuki
Yukio
Y
D001236:Aspergillus oryzae; D036145:Ginsenosides / Q000737:chemistry*; D006801:Humans; D010407:Penicillium; D008517:Phytotherapy; D028321:Plant Preparations / Q000737:chemistry*
D036145:Ginsenosides; D028321:Plant Preparations; C055327:ginsenoside F1; C026474:ginsenoside Rg2; C425564:ginsenoside Rh1