title: Drug-associated resistance mutations in plasma and peripheral blood mononuclear cells of human immunodeficiency virus type 1-infected patients for whom highly active antiretroviral therapy is failing.
abstract: In 32 patients for whom highly active antiretroviral therapy was failing, a good agreement between drug resistance-associated mutations in plasma and peripheral blood mononuclear cells (PBMCs) was found (k = 0.85). The mutations with the lowest agreement were 20R, 63P, and 84V in the protease gene and 184V in the reverse transcriptase gene. In eight patients, primary drug resistance mutations were detected only in PBMCs.
authors.affiliation: Department of Public Health, University of Rome Tor Vergata, Rome, Italy.
authors.forename: Loredana
authors.initials: L
authors.lastname: Sarmati
authors.forename: Emanuele
authors.initials: E
authors.lastname: Nicastri
authors.forename: Ilaria
authors.initials: I
authors.lastname: Uccella
authors.forename: Gabriella
authors.initials: G
authors.lastname: D'Ettorre
authors.forename: Saverio G
authors.initials: SG
authors.lastname: Parisi
authors.forename: Lucia
authors.initials: L
authors.lastname: Palmisano
authors.forename: Clementina
authors.initials: C
authors.lastname: Galluzzo
authors.forename: Ercole
authors.initials: E
authors.lastname: Concia
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Vullo
authors.forename: Stefano
authors.initials: S
authors.lastname: Vella
authors.forename: Massimo
authors.initials: M
authors.lastname: Andreoni
mesh_terms: D000328:Adult; D023241:Antiretroviral Therapy, Highly Active; D004279:DNA, Viral / Q000097:blood; D024882:Drug Resistance, Viral / Q000235:genetics*; D015658:HIV Infections / Q000188:drug therapy* / Q000821:virology; D016333:HIV Protease / Q000235:genetics; D054303:HIV Reverse Transcriptase / Q000235:genetics; D015497:HIV-1 / Q000145:classification / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology*; D008297:Male; D008875:Middle Aged; D009154:Mutation; D017211:Treatment Failure
chemical_list: D004279:DNA, Viral; D054303:HIV Reverse Transcriptase; D016333:HIV Protease