title: [Effects of burn serum on the viscoelasticity and the structure of rat intestinal epithelial cells].
abstract: OBJECTIVE
  To investigate the effects of burn serum on the viscoelasticity and the structure of rat intestinal epithelial cells.
  
  
  METHODS
  The rat intestinal epithelial cell strain (IEC-6) was cultured and stimulated by burn serum. The changes of IECs before and after the stimulation were dynamically observed by cytoskeleton immunohistochemistry, ELISA and the measurement of cytomembranous viscoelasticity.
  
  
  RESULTS
  During the early stage of burn serum stimulation, the skeleton protein expression in IEC decreased obviously with weakened positive signals of microfilaments and microtubules and with decreased cellular elasticity.
  
  
  CONCLUSIONS
  The cytoskeleton injury could cause the increase of cellular fragility and the decrease of the viscoelasticity, which ultimately lead to the change of cellular biodynamics. These changes might directly participate the development of postburn intestinal epithelial injury.
authors.affiliation: Institute of Burn Research, Southwestern Hospital, The Third Military Medical University, Chongqing 400038 P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Ao
authors.initials: A
authors.lastname: Tang
authors.forename: Ze-Zhi
authors.initials: ZZ
authors.lastname: Wu
authors.forename: Jian
authors.initials: J
authors.lastname: Qin
authors.forename: Guang-Xia
authors.initials: GX
authors.lastname: Xiao
mesh_terms: D000199:Actins / Q000032:analysis; D000818:Animals; D002056:Burns / Q000097:blood*; D002460:Cell Line; D003470:Culture Media / Q000494:pharmacology; D003598:Cytoskeletal Proteins / Q000187:drug effects / Q000378:metabolism*; D004548:Elasticity / Q000187:drug effects; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014404:Tubulin / Q000032:analysis; D014783:Viscosity / Q000187:drug effects
chemical_list: D000199:Actins; D003470:Culture Media; D003598:Cytoskeletal Proteins; D014404:Tubulin