Development and evaluation of the nuclisens basic kit NASBA for the detection of RNA from Candida species frequently resistant to antifungal drugs.
We describe a Nucleic Acid Sequence Based Amplification (NASBA) protocol to detect 6 different Candida species (Candida krusei, Candida glabrata, Candida inconspicua, Candida dubliniensis, Candida norvegensis, Candida lusitaniae) and compare it to a PCR assay. NASBA showed a sensitivity of 1 Colony Forming Unit and detected RNA from all 6 Candida species within 1 working day. All 5 patients with documented candidiasis showed identical results by both methods. This assay offers a sensitive, specific and fast possibility to detect yeast RNA.
Loeffler
Juergen
J
Eberhard-Karls-Universität, Medizinische Klinik, 72076, Tübingen, Germany. juergen.loeffler@med.uni-tuebingen.de <juergen.loeffler@med.uni-tuebingen.de>
Dorn
Christiane
C
Hebart
Holger
H
Cox
Philipp
P
Magga
Stella
S
Einsele
Hermann
H
D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000145:classification* / Q000187:drug effects*; D002177:Candidiasis / Q000175:diagnosis / Q000188:drug therapy; D025141:Drug Resistance, Fungal; D005260:Female; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D016533:Mycological Typing Techniques; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012331:RNA, Fungal / Q000032:analysis*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
D000935:Antifungal Agents; D012331:RNA, Fungal; D011933:Reagent Kits, Diagnostic