Visualizing vitreous using Kenalog suspension.
We developed and evaluated a method of visualizing vitreous gel in the anterior segment. In this study, 0.2 mL of injectable triamcinolone (Kenalog) 40 mg/mL was captured in a 5 microm filter and rinsed with 2 mL of balanced salt solution (BSS). It was then resuspended in 5 mL of BSS and recaptured to thoroughly remove the preservative. The Kenalog particles were ultimately resuspended in 2 mL of BSS and injected into the anterior chamber through a 27-gauge cannula. Kenalog particles were trapped on and within the vitreous gel, making it clearly visible. The visualization provided direct observation of vitreous behavior in various experimental settings and assisted surgeons intraoperatively in the identification and removal of vitreous in the anterior segment.
Burk
Scott E
SE
Cincinnati Eye Institute, Ohio 45242, USA.
Da Mata
Andrea P
AP
Snyder
Michael E
ME
Schneider
Susan
S
Osher
Robert H
RH
Cionni
Robert J
RJ
D000085:Acetates; D000328:Adult; D000867:Anterior Chamber / Q000187:drug effects / Q000473:pathology*; D004338:Drug Combinations; D005128:Eye Diseases / Q000175:diagnosis* / Q000209:etiology / Q000601:surgery; D005131:Eye Injuries / Q000150:complications / Q000601:surgery; D005260:Female; D005938:Glucocorticoids; D006801:Humans; D008297:Male; D008875:Middle Aged; D008903:Minerals; D012965:Sodium Chloride; D013535:Suspensions; D014222:Triamcinolone Acetonide; D014821:Vitrectomy / Q000379:methods*; D014822:Vitreous Body / Q000473:pathology* / Q000601:surgery
D000085:Acetates; C030660:BSS solution; D004338:Drug Combinations; D005938:Glucocorticoids; D008903:Minerals; D013535:Suspensions; D012965:Sodium Chloride; D014222:Triamcinolone Acetonide