title: Simple, rapid, and automated method for detection of hyperaggregability of platelets using a hematology analyzer.
abstract: Estimation of hyperaggregability of platelets is important for diagnosis and prevention of vascular events. We have developed and evaluated a simple and rapid method for detection of a hyperaggregable state of platelets by using an Abbott CELL-DYN(R) 4000 hematology analyzer. Citrated blood samples were collected from 62 patients with chronic cerebral infarction (CCI), of whom 19 patients were treated with ticlopidine, and from 9 healthy subjects. Platelet clumps were detected in the scatter plots for white blood cell populations with the hematology analyzer. Platelet clumps were positive in 20 of 43 (46.5%) CCI patients who were not treated with anti-platelet agents but not at all in 9 healthy subjects and in 19 CCI patients treated with ticlopidine. The detection of platelet clumps in citrated blood by the hematology analyzer was proved useful in detecting a platelet hyperaggregability in CCI patients. This method is simple, rapid, and automated and thus should be suitable for routine clinical use for monitoring indications and the efficacy of anti-platelet drugs.
authors.affiliation: Department of Laboratory Medicine, Tokai University School of Medicine, Isehara, Japan.
authors.forename: Mie
authors.initials: M
authors.lastname: Shimizu
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Hayato
authors.initials: H
authors.lastname: Miyachi
authors.forename: Yukito
authors.initials: Y
authors.lastname: Shinohara
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Ando
mesh_terms: D000368:Aged; D000925:Anticoagulants / Q000494:pharmacology / Q000627:therapeutic use; D001331:Automation; D001772:Blood Cell Count / Q000295:instrumentation; D002544:Cerebral Infarction / Q000097:blood / Q000188:drug therapy; D002908:Chronic Disease; D019343:Citric Acid / Q000494:pharmacology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010974:Platelet Aggregation / Q000187:drug effects; D010979:Platelet Function Tests / Q000295:instrumentation*; D013988:Ticlopidine / Q000627:therapeutic use; D013997:Time Factors
chemical_list: D000925:Anticoagulants; D019343:Citric Acid; D013988:Ticlopidine