[Determination of alkaloids from Coptis chinensis Franch. and Phellodendron amurense Rupr. Decocted together in baitouweng decocta by high performance capillary electrophoresis].
A rapid and accurate method for determining alkaloids from Coptis chinensis Franch, and Phellodendron amurense Rupr, decocted together in Baitouweng decocta was established. Experiments were carried out on a self-assembled capillary electrophoresis system. The experimental conditions were as follows: 75 microns i.d. x 50 cm fused silica capillary, 0.05 mol/L Na2B4O7-CH3OH (85:15, V/V) as buffer, applied voltage 14 kV and detection wavelength 232 nm. In addition, the concentration of ethanol in the extraction solvent was optimized. Experimental results showed that ethanol-water (30:70, V/V) was the ideal solvent to extract the main effective ingredients from Coptis chinensis Franch. and Phellodendron amurense Rupr. decocted together in Baitouweng decocta. Berberine and palmatine had good linearities in the range of 15.0 mg/L-65.0 mg/L and 12.5 mg/L-50.0 mg/L respectively, and their average recoveries were 95.5%-104% (RSD 2.7%-6.9%) and 92.1%-103% (RSD 4.5%-6.7%) respectively.
Wei
Pei-hai
PH
Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730020, China.
Chen
Li-ren
LR
Wei
Ying-qin
YQ
Yuan
Jiu-rong
JR
Ma
Ji-ping
JP
Chen
Ling-xin
LX
D000470:Alkaloids / Q000032:analysis*; D001599:Berberine / Q000032:analysis; D001600:Berberine Alkaloids / Q000032:analysis; D039323:Coptis / Q000737:chemistry*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods; D032102:Phellodendron / Q000737:chemistry*; D012995:Solubility
D000470:Alkaloids; D001600:Berberine Alkaloids; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D001599:Berberine; C005413:palmatine