title: High-level triclosan resistance in Pseudomonas aeruginosa is solely a result of efflux.
abstract: BACKGROUND
  Pseudomonas aeruginosa is intrinsically resistant to high levels of triclosan.
  
  
  METHODS
  Using the agar incorporation method and defined mutant strains, the contribution of multidrug efflux pumps to high-level (>1,000 microg/mL) triclosan resistance in P aeruginosa was assessed.
  
  
  RESULTS
  The results showed that the ability of P aeruginosa to survive in the presence of triclosan concentrations in excess of 1,000 microg/mL is solely attributable to the expression of efflux pumps.
authors.affiliation: Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, USA.
authors.forename: Rungtip
authors.initials: R
authors.lastname: Chuanchuen
authors.forename: RoxAnn R
authors.initials: RR
authors.lastname: Karkhoff-Schweizer
authors.forename: Herbert P
authors.initials: HP
authors.lastname: Schweizer
mesh_terms: D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D015169:Colony Count, Microbial; D003470:Culture Media; D024881:Drug Resistance, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D014260:Triclosan / Q000494:pharmacology*
chemical_list: D000891:Anti-Infective Agents, Local; D003470:Culture Media; D014260:Triclosan