title: Effects of Schistosoma mansoni infection on somatostatin and somatostatin receptor 2A expression in mouse ileum.
abstract: Intestinal schistosomiasis is accompanied by motility-related dysfunctions but the underlying mechanisms are not well-known. Therefore, the presence and effects on intestinal contractility of somatostatin (SOM) and its receptor, SSTR2A, were investigated in the ileum of normal and infected mice. The distribution of SOM and SSTR2A was visualized using immunocytochemistry. Radioimmunoassay combined with oogram studies was performed to determine SOM levels and contractility measurements were determined in organ bath experiments. Schistosomiasis resulted in a significant decrease in somatostatin-positive endocrine cells, whereas the number of somatostatin-immunoreactive (IR) neuronal cell bodies did not change. From 8 weeks postinfection onwards, an increase was noted in somatostatin-IR nerve fibres in both villi and granulomas. The staining intensity for SSTR2A, expressed in somatostatin-negative myenteric cholinergic neurones, increased during infection suggesting an upregulation of this receptor. SOM levels were negatively correlated with the number of eggs during the acute phase, and were elevated during the chronic phase. Pharmacological experiments revealed that schistosomiasis diminished the inhibitory effect of SOM on neurogenic contractions. We can conclude that schistosomiasis influences the distribution and expression levels of SOM and SSTR2A in the murine ileum, which might explain the changed motility pattern.
authors.affiliation: Laboratory of Cell Biology and Histology, Department of Biomedical Sciences, University of Antwerp, Belgium.
authors.forename: F
authors.initials: F
authors.lastname: De Jonge
authors.forename: L
authors.initials: L
authors.lastname: Van Nassauw
authors.forename: J G
authors.initials: JG
authors.lastname: De Man
authors.forename: B Y
authors.initials: BY
authors.lastname: De Winter
authors.forename: F
authors.initials: F
authors.lastname: Van Meir
authors.forename: I
authors.initials: I
authors.lastname: Depoortere
authors.forename: T L
authors.initials: TL
authors.lastname: Peeters
authors.forename: P A
authors.initials: PA
authors.lastname: Pelckmans
authors.forename: E
authors.initials: E
authors.lastname: Van Marck
authors.forename: J-P
authors.initials: JP
authors.lastname: Timmermans
mesh_terms: D000320:Adrenergic Fibers / Q000378:metabolism / Q000469:parasitology; D000818:Animals; D002799:Cholinergic Fibers / Q000378:metabolism / Q000469:parasitology; D004558:Electric Stimulation; D005769:Gastrointestinal Motility / Q000502:physiology; D007082:Ileum / Q000166:cytology / Q000294:innervation / Q000378:metabolism / Q000469:parasitology*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D009119:Muscle Contraction / Q000502:physiology; D009924:Organ Culture Techniques; D011863:Radioimmunoassay; D017481:Receptors, Somatostatin / Q000096:biosynthesis*; D012550:Schistosoma mansoni; D012555:Schistosomiasis mansoni / Q000378:metabolism* / Q000503:physiopathology; D013004:Somatostatin / Q000096:biosynthesis*; D013997:Time Factors; D015854:Up-Regulation
chemical_list: D017481:Receptors, Somatostatin; D013004:Somatostatin; C093733:somatostatin receptor 2