Interleukin-17 increases the effects of IL-1 beta on muscle cells: arguments for the role of T cells in the pathogenesis of myositis.
We studied the production of interleukin (IL)-6 and CCL20/macrophage inflammatory protein-3 alpha (MIP-3 alpha) by human myoblasts and muscle samples in response to IL-17 alone or in combination with IL-1 beta. Both IL-17 and IL-1 beta induced IL-6 production by normal myoblasts and muscle samples. IL-17 had no effect on CCL20 production by myoblasts. Combination of IL-17 and IL-1 beta further increased IL-6 and CCL20 production by muscle samples but not that of CK. IL-17 induced also HLA class I, C-Fos, nuclear factor kappa B (NF-kappa B) and C-Jun expression by myoblasts but not that of HLA class II, CD40, vascular cell adhesion molecule 1 (VCAM-1) and intercellular adhesion molecule 1 (ICAM-1). Finally, immunostaining of dermatomyositis (DM) and polymyositis (PM) muscle biopsies showed IL-17 and CCL20 expression. Our study shows that low levels of cytokines produced by T cells (IL-17) and monocytes (IL-1 beta) can act in combination on skeletal myoblasts and muscle tissue.
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Department of Immunology and Rheumatology, Hôpital E. Herriot, 5 place d'Arsonval, 69437 Lyon Cedex 03, France.
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Streichenberger
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Varennes
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D000276:Adjuvants, Immunologic / Q000494:pharmacology / Q000502:physiology; D016207:Cytokines / Q000096:biosynthesis; D004338:Drug Combinations; D004357:Drug Synergism; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology / Q000502:physiology*; D020381:Interleukin-17 / Q000494:pharmacology / Q000502:physiology*; D018482:Muscle, Skeletal / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D032448:Myoblasts, Skeletal / Q000276:immunology / Q000378:metabolism; D009220:Myositis / Q000276:immunology* / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
D000276:Adjuvants, Immunologic; D016207:Cytokines; D004338:Drug Combinations; D007375:Interleukin-1; D020381:Interleukin-17