title: Prenatal diagnosis of congenital human cytomegalovirus infection in amniotic fluid by nucleic acid sequence-based amplification assay.
abstract: Nucleic acid sequence-based amplification assays for detection of human cytomegalovirus (HCMV) immediate-early and pp67 mRNA in 65 amniotic fluid samples tested for prenatal diagnosis of congenital HCMV infection showed sensitivity, specificity, and negative and positive predictive values >90%.
authors.affiliation: Servizio di Virologia, Istituto di Ricovero e Cura a Carattere Scientifico Policlinico San Matteo, 27100 Pavia, Italy.
authors.forename: M Grazia
authors.initials: MG
authors.lastname: Revello
authors.forename: Daniele
authors.initials: D
authors.lastname: Lilleri
authors.forename: Maurizio
authors.initials: M
authors.lastname: Zavattoni
authors.forename: Milena
authors.initials: M
authors.lastname: Furione
authors.forename: Jaap
authors.initials: J
authors.lastname: Middeldorp
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Gerna
mesh_terms: D000653:Amniotic Fluid / Q000821:virology*; D003587:Cytomegalovirus / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000151:congenital / Q000175:diagnosis* / Q000821:virology; D005260:Female; D005315:Fetal Diseases / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D011237:Predictive Value of Tests; D011247:Pregnancy; D011296:Prenatal Diagnosis; D021161:Self-Sustained Sequence Replication; D012680:Sensitivity and Specificity