Collagen secretion after photodynamic therapy versus scar-inducing anti-cancer modalities: an in vitro study.
Photodynamic therapy (PDT) has been associated anecdotally with good quality healing and an absence of scar formation. Our previous studies, examining the levels of the collagen specific molecular chaperone Hsp47, have noted differences in the response after photodynamic therapy and hyperthermia at both the transcriptional and translational levels. In the present study the levels of Hsp47 after exposure to two chemotherapeutic agents (bleomycin and mitomycin). ionising radiation, hyperthermia and haematoporphyrin ester (HpE) mediated PDT were compared in both mouse and human fibroblast cell lines. A rapid assay for soluble collagen has also been used to quantify soluble collagen levels at early time points after treatment. Peak Hsp47 levels were found to correlate well with peak collagen levels. The results show that the levels of collagen measured in vitro are elevated in modalities associated with scarring in vivo but not after HpE-PDT.
Haylett
Ann K
AK
Cancer Research-UK Laser Oncology Group, Paterson Institute for Cancer Research, Wilmslow Rd, Manchester, UK M20 4BX. ahaylett@picr.man.ac.uk
Higley
Katherine
K
Chiu
Maybo
M
Shackley
David C
DC
Moore
James V
JV
D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D001761:Bleomycin / Q000633:toxicity; D002460:Cell Line; D002921:Cicatrix / Q000139:chemically induced; D003094:Collagen / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D005720:Gamma Rays; D050958:HSP47 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000378:metabolism; D006415:Hematoporphyrins / Q000494:pharmacology*; D006358:Hot Temperature; D007700:Kinetics; D051379:Mice; D016685:Mitomycin / Q000633:toxicity; D010778:Photochemotherapy
D000970:Antineoplastic Agents; D050958:HSP47 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D006415:Hematoporphyrins; C494661:Serpinh1 protein, mouse; C403716:hematoporphyrin diethyl ether; D001761:Bleomycin; D016685:Mitomycin; D003094:Collagen