[Cell interaction with extracellular proteins during two-phase polymer system formation].
A modified method of investigation of surface properties of cells and proteins with the help of a two-phase polymer system dextran-500/polyethylenglycol-6000 was used. This method is based on changing the kinetics of two-phase system partitioning into phase on adding cells or macromolecules. These changes were registered by measuring the top phase optical density during the system partitioning at 500 nm. Cell lines L (NCTC clone 929), LS and A431, human keratinocytes and platelets, collagen I, laminin-1, laminin-2/4, and fibronectin were studied. The interaction between collagen and all cell types with the formation of complexes takes place during co-partitioning of cells and proteins in the two-phase system. Laminins differ in surface properties and in interaction with cells. Laminin-1 makes preferable complexes with cells of monolayer subline L (NCTC clone 929), but not with cells of suspension subline LS. No interaction of laminin-2/4 with L cells was detected, but, in contrast to laminin-1, this protein has the affinity to A431 cells. No interaction of L cells with fibronectin were detected.
Voronkina
I V
IV
Institute of Cytology RAS, St. Petersburg. voron@mail.cytsb.tssi.ru
Kalmykova
N V
NV
Petrov
Iu P
IuP
Pinaev
G P
GP
Tsupkina
N V
NV
Cherepanova
O A
OA
D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D003094:Collagen / Q000378:metabolism; D003377:Countercurrent Distribution; D003911:Dextrans; D004912:Erythrocytes / Q000166:cytology* / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000378:metabolism; D007797:Laminin / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D011092:Polyethylene Glycols
D003911:Dextrans; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D007797:Laminin; D011092:Polyethylene Glycols; D003094:Collagen