title: Regulation of cytokine mRNAs by interferon and interferon inducers.
abstract: Cytokine mRNA expression was studied in human long-term cell cultures of different origin: J-96 and J-41 (monocytic leukemia), SW-13 (paradrenal adenocarcinoma), and MT-4 (T-cell leukemia), in response to IFN-alpha and IFN inducers (kagocel and cycloferon). Cytokine mRNA level in the cell cultures was measured by the RT-PCR method using 11 primer pairs for the following cytokines: IFN-alpha, IFN-gamma, IL-1beta, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-18 and TNF-alpha. It was shown that IFN-alpha and IFN inducers possess an ability to regulate different cytokine mRNAs. Treatment of the cells with IFN-alpha resulted in expression of mRNAs for IL-2, IL-4, and IL-8. Kagocel induced production of IFN-alpha, IFN-gamma, and IL-2 mRNAs, and cycloferon--IFN-gamma, IL-2, IL-4, and IL-8 mRNAs. It is suggested that antiviral effects of these inducers, in general, can be attributed to imitation of cytokine responses observed in viral infection and, as a result, can lead to starting-up of cellular defense antiviral mechanisms even before action of viruses. Conclusion is made that IFN and IFN inducers may act as regulators of cytokine activity.
authors.affiliation: N.F. Gamaleya Institute for Epidemiology and Microbiology, Russian Academy of Medical Sciences, Gamaleya str. 18, Moscow 123098, Russia. narovl@mail.infotel.ru
authors.forename: Maria Yu
authors.initials: MY
authors.lastname: Vershinina
authors.forename: Alexander N
authors.initials: AN
authors.lastname: Narovlyansky
authors.forename: Pietr G
authors.initials: PG
authors.lastname: Deryabin
authors.forename: Aleftina M
authors.initials: AM
authors.lastname: Amchenkova
authors.forename: Alla M
authors.initials: AM
authors.lastname: Ivanova
authors.forename: Vakhtang E
authors.initials: VE
authors.lastname: Scherbenko
authors.forename: Elena V
authors.initials: EV
authors.lastname: Nagurskaya
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Bechalo
authors.forename: Tatiana Yu
authors.initials: TY
authors.lastname: Timofeeva
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Sanin
authors.forename: Felix I
authors.initials: FI
authors.lastname: Ershov
mesh_terms: D000166:Acridines / Q000494:pharmacology; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics*; D006072:Gossypol / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D007369:Interferon Inducers / Q000378:metabolism* / Q000494:pharmacology; D007372:Interferons / Q000378:metabolism* / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000187:drug effects / Q000235:genetics
chemical_list: D000166:Acridines; D016207:Cytokines; D007369:Interferon Inducers; D012333:RNA, Messenger; C056991:cagocel-1; D007372:Interferons; D006072:Gossypol; C026430:10-carboxymethyl-9-acridanone