Observation of Fe-H/D modes by nuclear resonant vibrational spectroscopy.
Metal-hydrogen bonding is important in chemistry and catalysis, but H atoms are often difficult to observe, especially in metalloproteins. In this work we show that Fe-H interactions can be probed by nuclear resonance vibrational spectroscopy at the 14.4 keV 57Fe nuclear resonance. An important advantage of this method, compared to Raman and IR spectroscopy, is the selectivity for modes that involve 57Fe motion. We present data on the FeS4 site in rubredoxin and the [FeH(D)6]2- ion. Prospects for studying more complex systems are discussed.
Bergmann
Uwe
U
Department of Applied Science, University of California, Davis, California 95616, USA.
Sturhahn
Wolfgang
W
Linn
Donald E
DE
Jenney
Francis E
FE
Adams
Michael W W
MW
Rupnik
Kresimir
K
Hales
Brian J
BJ
Alp
Ercan E
EE
Mayse
Aaron
A
Cramer
Stephen P
SP
D006859:Hydrogen / Q000737:chemistry*; D006864:Hydrogenase / Q000737:chemistry*; D058085:Iron Compounds / Q000737:chemistry*; D009591:Nitrogenase / Q000737:chemistry*; D020027:Pyrococcus furiosus / Q000737:chemistry; D012416:Rubredoxins / Q000737:chemistry; D012542:Scattering, Radiation; D013057:Spectrum Analysis / Q000379:methods; D013440:Sulfides / Q000737:chemistry; D014732:Vibration; D014965:X-Rays
D058085:Iron Compounds; D012416:Rubredoxins; D013440:Sulfides; D006859:Hydrogen; D006864:Hydrogenase; D009591:Nitrogenase