title: Study of rat osteoblasts transfected by transforming growth factor beta 1 gene.
abstract: In order to investigate the effect of TGF beta 1 gene transfer on the biological characteristics, the effects of gene transfer and supernatant of transfected osteoblasts on the proliferation and ALP activity of osteoblasts were detected by 3H-TdR and MTT. Our results showed that TGF beta 1 gene transfer had no effect on the biological characteristics and the activated supernatant of transfected osteoblasts stimulated proliferation and inhibited ALP activity of osteoblasts. TGF beta 1 gene transfer could promote the expression of TGF beta 1 and the biological characteristics of transfected osteoblasts were stable, which might be helpful for gene therapy of bone defects in vivo.
authors.affiliation: Department of Orthopedics, Xiehe Hospital, Tongji Medical University, Huazhong University of Science and Technology, Wuhan 430022.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Jingyuan
authors.initials: J
authors.lastname: Du
authors.forename: Qixin
authors.initials: Q
authors.lastname: Zheng
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Guo
authors.forename: Deyu
authors.initials: D
authors.lastname: Duan
authors.forename: Weigang
authors.initials: W
authors.lastname: Liu
mesh_terms: D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000235:genetics; D018014:Gene Transfer Techniques; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D023822:Tissue Engineering; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta