title: Kinetics of unfolding the human telomeric DNA quadruplex using a PNA trap.
abstract: The kinetics of opening of the DNA quadruplex formed by the human telomeric repeat have been investigated using real-time fluorescence resonance energy transfer (FRET) measurements with a peptide nucleic acid (PNA) trap. It has been found that this opening is zero-order with respect to PNA, indicating that the initial step is a rate-limiting internal rearrangement of the quadruplex. A study of the temperature dependence of the rate of quadruplex opening was performed and the activation energy of the process estimated to be 98 +/- 8 kJ mol(-1).
authors.affiliation: Department of Chemistry, University of Cambridge, Lensfield Road, UK.
authors.forename: Jeremy J
authors.initials: JJ
authors.lastname: Green
authors.forename: Liming
authors.initials: L
authors.lastname: Ying
authors.forename: David
authors.initials: D
authors.lastname: Klenerman
authors.forename: Shankar
authors.initials: S
authors.lastname: Balasubramanian
mesh_terms: D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D054856:G-Quadruplexes; D006801:Humans; D007700:Kinetics; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D016615:Telomere / Q000737:chemistry* / Q000235:genetics
chemical_list: D009841:Oligonucleotides; D020135:Peptide Nucleic Acids; D004247:DNA