In vitro oxidative activity of cupric complexes of kanamycin A in comparison to in vivo bactericidal efficacy.
The interactions of copper(II) complexes of kanamycin A with oxidation-susceptible biomolecules: 2'-deoxyguanosine, plasmid DNA and yeast tRNA(Phe) were studied in both the presence and absence of hydrogen peroxide. The mixture of complex with H(2)O(2) was found to be an efficient oxidant, converting dG to its 8-oxo derivative, generating strand breaks in plasmid DNA and multiple cleavages in tRNA(Phe). Some of these reactions may play a role in toxic effects of aminoglycoside antibiotics. These complexes were screened for their antibacterial activity. The microbiological studies undertaken to compare the bactericidal action of kanamycin A alone and complexed with copper(II) ions in both neutral and oxidative environment revealed that the enhancement of bactericidal action by Cu(II) was not statistically significant.
Szczepanik
Wojciech
W
Faculty of Chemistry, University of Wroclaw, F Joliot-Curie 14, 50-383 Wrocław, Poland.
Dworniczek
Ewa
E
Ciesiołka
Jerzy
J
Wrzesiński
Jan
J
Skala
Jacek
J
Jezowska-Bojczuk
Malgorzata
M
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology*; D001345:Autoradiography; D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000737:chemistry / Q000187:drug effects; D004249:DNA Damage; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000187:drug effects; D003849:Deoxyguanosine / Q000031:analogs & derivatives; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000737:chemistry; D007612:Kanamycin / Q000737:chemistry*; D007700:Kinetics; D009942:Organometallic Compounds / Q000737:chemistry* / Q000494:pharmacology*; D010084:Oxidation-Reduction; D010957:Plasmids / Q000187:drug effects; D012343:RNA, Transfer / Q000737:chemistry / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013211:Staphylococcus aureus / Q000187:drug effects
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D004277:DNA, Single-Stranded; D009942:Organometallic Compounds; D007612:Kanamycin; D003300:Copper; D004247:DNA; D012343:RNA, Transfer; D006861:Hydrogen Peroxide; D003849:Deoxyguanosine