title: Adaptation of an automated assay for determination of beta-hydroxybutyrate in dogs using a random access analyzer.
abstract: An automated method for measuring beta-hydroxybutyrate was adapted to the Ciba-Corning 550 Express trade mark random access analyzer. The assay was based on a kinetic reaction utilizing hydroxybutyrate-dehydrogenase. Beta-hydroxybutyrate concentration (mmol/L) was calculated ratiometrically using a 1.0 mmol/l standard. Canine serum, plasma, and urine were used without prior deproteinization and only a 30-microliter sample was required. The method demonstrated good linearity between 0 to 2 mmol/l of beta-hydroxybutyrate. Analytical recovery (accuracy) within these concentrations ranged from 85.8 to 113.3%. Both within-run and day-to-day precision were determined, as was specificity of the assay in the presence of a variety of interfering substances. The automated assay was rapid and economical, with reagent stability maintained for at least 2 weeks at 4 degrees C. This assay can readily be applied toward the assessment of ketoacidosis in dogs, and with further validation, other species.
authors.affiliation: Department of Physiological Sciences, College of Veterinary Medicine, P.O. Box 100-144, Gainesville, FL 32610.
authors.forename: Mary M.
authors.initials: MM
authors.lastname: Christopher
authors.forename: Jacqueline L.
authors.initials: JL
authors.lastname: Pereira
authors.forename: Robin L.
authors.initials: RL
authors.lastname: Brigmon