Passive immunization with monoclonal antibodies: effects on Haemaphysalis longicornis tick infestation of BALB/c mice.
Tick vaccine development plays an important role in current tick control strategies. Previously, we have produced three different isotypes of monoclonal antibodies (mAbs) which recognized a midgut protein of adult Haemaphysalis longicornis. These mAbs, typed as IgG1, 2a, and 2b, reacted with a 76 kDa surface protein of midgut cells. We speculated that the 76 kDa protein may be an unknown antigen for a tick vaccine and the three mAbs may work as probes to clone the protein. In this study, to test whether these three isotypes have anti-tick effects and if so which works more effectively, we conducted passive immunization in BALB/c mice with each of the mAbs, and infested the mice with adult ticks. All isotypes significantly reduced the number of hatched larvae, compared to controls, however, no differences in the magnitude of the reduction were observed among the three.
Nakajima
Mie
M
Laboratory of Infectious Diseases, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Yanase
Haruko
H
Iwanaga
Toshihiko
T
Kodama
Michi
M
Ohashi
Kazuhiko
K
Onuma
Misao
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D005260:Female; D007116:Immunization, Passive / Q000379:methods*; D007132:Immunoglobulin Isotypes / Q000276:immunology; D019476:Insect Proteins / Q000276:immunology*; D026863:Ixodidae / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013984:Tick Infestations / Q000517:prevention & control*
D000911:Antibodies, Monoclonal; D007132:Immunoglobulin Isotypes; D019476:Insect Proteins