title: Protective effect of Mangifera indica L. extract (Vimang) on the injury associated with hepatic ischaemia reperfusion.
abstract: The effect of Mangifera indica L. extract (Vimang) on treatment of injury associated with hepatic ischaemia/reperfusion was tested. Vimang protects from the oxidative damage induced by oxygen-based free radicals as shown in several in vitro test systems conducted. The ability of Vimang to reduce liver damage was investigated in rats undergoing right-lobe blood fl ow occlusion for 45 min followed by 45 min of reperfusion. The ischaemia/reperfusion model leads to an increase of transaminase (ALT and AST), membrane lipid peroxidation, tissue neutrophil in filtration, DNA fragmentation, loss of protein -SH groups, cytosolic Ca2+ overload and a decrease of catalase activity. Oral administration of Vimang (50, 110 and 250 mg/kg, b.w.) 7 days before reperfusion, reduced transaminase levels and DNA fragmentation in a dose dependent manner (p < 0.05). Vimang also restored the cytosolic Ca2+ levels and inhibited polymorphonuclear migration at a dose of 250 mg/kg b.w., improved the oxidation of total and non protein sulfhydryl groups and prevented modification in catalase activity, uric acid and lipid peroxidation markers (p < 0.05). These data suggest that Vimang could be a useful new natural drug for preventing oxidative damage during hepatic injury associated with free radical generation.
authors.affiliation: Centre for Research and Biological Evaluation, Pharmacy Institute, Havana University, San Lazaro y L, Havana 4, Cuba.
authors.forename: Gregorio Martínez
authors.initials: GM
authors.lastname: Sánchez
authors.forename: María A
authors.initials: MA
authors.lastname: Rodríguez H
authors.forename: Attilia
authors.initials: A
authors.lastname: Giuliani
authors.forename: Alberto J
authors.initials: AJ
authors.lastname: Núñez Sellés
authors.forename: Niurka Pons
authors.initials: NP
authors.lastname: Rodríguez
authors.forename: Olga Sonia
authors.initials: OS
authors.lastname: León Fernández
authors.forename: L
authors.initials: L
authors.lastname: Re
mesh_terms: D000284:Administration, Oral; D000410:Alanine Transaminase / Q000097:blood / Q000187:drug effects; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D001219:Aspartate Aminotransferases / Q000097:blood / Q000187:drug effects; D005260:Female; D007511:Ischemia / Q000188:drug therapy*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000098:blood supply* / Q000187:drug effects / Q000201:enzymology; D008111:Liver Function Tests; D031022:Mangifera; D008517:Phytotherapy; D024301:Plant Bark; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase