title: Immunohistochemical localization of sodium-potassium ATPase in human normal stomach and gastric adenocarcinomas.
abstract: We studied the expression pattern of Na, K-ATPase beta 1 subunit in human normal stomachs and in gastric adenocarcinomas by using anti-Na, K-ATPase beta 1 subunit-specific monoclonal antibody. Tissue samples were processed in formalin solution or in a cold acetic acid-ethanol solution, routinely processed, embedded in paraffin, and an immunoperoxidase method for Na, K-ATPase beta 1 subunit was performed. After antigen retrieval using a steamer in citrate buffer (pH 6.0), tissue sections initially fixed in cold acetic acid-ethanol showed intense immunoreactivity with the antibody at the lateral or basolateral cytoplasmic membrane of normal gastric epithelial cells, at the cytoplasmic membrane of gastric carcinoma cells according to the level of differentiation, and at the cytoplasmic membrane and in the cytoplasm of Schwann cells and neurons in the mesenteric plexus of the gastric wall. Acetic acid-ethanol and paraffin embedding is a useful method for the investigation of the immunohistochemical localization of Na, K-ATPase in normal and diseased tissues.
authors.affiliation: First Department of Surgery, School of Medicine, Shinshu University, Matsumoto, 390-8621 Nagano, Japan.
authors.forename: Chujiro
authors.initials: C
authors.lastname: Kobayashi
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Hayama
authors.forename: Oi
authors.initials: O
authors.lastname: Harada
authors.forename: Fumiko
authors.initials: F
authors.lastname: Terasawa
authors.forename: Nobuo
authors.initials: N
authors.lastname: Okumura
authors.forename: Atsushi
authors.initials: A
authors.lastname: Sugiyama
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Ota
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology* / Q000473:pathology; D005753:Gastric Mucosa / Q000033:anatomy & histology / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D015151:Immunoblotting; D007124:Immunoenzyme Techniques / Q000379:methods*; D007527:Isoenzymes; D000254:Sodium-Potassium-Exchanging ATPase / Q000276:immunology / Q000378:metabolism*; D013270:Stomach / Q000033:anatomy & histology / Q000201:enzymology*; D013274:Stomach Neoplasms / Q000201:enzymology* / Q000473:pathology
chemical_list: D007527:Isoenzymes; D000254:Sodium-Potassium-Exchanging ATPase