Antioxidative effect of chitosan on chronic carbon tetrachloride induced hepatic injury in rats.
Carbon tetrachloride (CCl(4)) is a toxic material known to induce lipid peroxidation and liver damage. To determine if chitosan has antioxidative effects on CCl(4)-induced liver injury, we administered 1 ml/kg of CCl(4) resolved in a 50% corn oil solution to rats every week by intraperitoneal injection. Chitosan (200 mg/kg body weight per day, MW 380,000 Da) was administered to the CCl(4) + chitosan treated rats by oral gavage during the experimental period. Chitosan significantly decreased liver thiobarbituric acid reactive substances (TBARS) and increased antioxidant enzyme activities (catalase and superoxide dismutase (SOD)). Fatty acid composition was not remarkably changed by chitosan; only arachidonic acid (20:4n-6) levels were significantly altered by CCl(4). Chitosan administration in the present experiment did not restore the decreased delta5-desaturase activity. In addition, chitosan supplementation did not prevent the CCl(4) induced degradation of CYP2E1. In conclusion, our results suggest that chitosan has antioxidative but not detoxifying effects on chronic CCl(4) induced hepatic injury in rats.
Jeon
Tae Il
TI
Department of Applied Biology and Chemistry, Konkuk University, 1 Hwayang-dong, Kwanghin-gu, Seoul 143-701, South Korea.
Hwang
Seong Gu
SG
Park
Nam Gyu
NG
Jung
Yu Ri
YR
Shin
Soo Im
SI
Choi
Sung Don
SD
Park
Dong Ki
DK
D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000627:therapeutic use*; D001835:Body Weight / Q000187:drug effects; D002252:Carbon Tetrachloride Poisoning / Q000188:drug therapy* / Q000201:enzymology / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002686:Chitin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000627:therapeutic use*; D048271:Chitosan; D019392:Cytochrome P-450 CYP2E1 / Q000096:biosynthesis; D004195:Disease Models, Animal; D007274:Injections, Intraperitoneal; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D000975:Antioxidants; D017392:Thiobarbituric Acid Reactive Substances; D002686:Chitin; D048271:Chitosan; D002374:Catalase; D019392:Cytochrome P-450 CYP2E1