Quantification of multiple mRNA levels in rat brain regions using real time optical PCR.
TaqMan is significantly more sensitive than other methods of mRNA quantification and makes possible the simultaneous analysis of numerous genes in small brain regions. This technique was used to quantify levels of mRNAs of 21 genes in tissue extracts from caudate putamen and nucleus accumbens from individual rats after 1 day 'binge' cocaine or saline administration. Expression of glyceraldehyde-3-phosphate-dehydrogenase, cyclophilin and actin mRNAs as well as 18S ribosomal RNA were evaluated for normalization of levels of gene expression.
Proudnikov
Dmitri
D
The Rockefeller University, Laboratory of the Biology of Addictive Diseases, 1230 York Avenue, New York, NY 10021, USA. proudnd@mail.rockefeller.edu
Yuferov
Vadim
V
LaForge
K Steven
KS
Ho
Ann
A
Jeanne Kreek
Mary
M
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D003042:Cocaine / Q000494:pharmacology*; D019970:Cocaine-Related Disorders / Q000235:genetics / Q000378:metabolism*; D005456:Fluorescent Dyes; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis* / Q000187:drug effects; D051381:Rats; D011916:Rats, Inbred F344; D015203:Reproducibility of Results; D013997:Time Factors
D005456:Fluorescent Dyes; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D003042:Cocaine