Alternative splicing modulation by a LAMMER kinase impinges on developmental and transcriptome expression.
Alternative splicing is a major contributor to genome complexity, playing a significant role in various cellular functions, including signal transduction, immunity, and development. The spliceosomal machinery is responsible for the processing of nuclear RNA. Several splicing factors associated with this complex are phosphorylated by kinases that possess a conserved LAMMER motif. We demonstrate in BY-2 tobacco cells a novel role for the LAMMER motif in the maintenance of proper subnuclear localization. Furthermore, high expression of the LAMMER kinase in Arabidopsis plants modulated the alternative splicing of specific endogenous genes and resulted in abnormal plant development and a novel transcriptome profile. A prominent feature was the upregulation of genes that play a role in protein turnover, suggesting a moderating function for these gene products in the control of alternative splicing events. Together, these results demonstrate alternative splicing modulation as a result of phosphorylation activity, providing an opportunity to study its global effect on the plasticity of plant development and gene expression at the organism level.
Savaldi-Goldstein
Sigal
S
Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel.
Aviv
Dvora
D
Davydov
Olga
O
Fluhr
Robert
R
D017398:Alternative Splicing / Q000235:genetics*; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D010940:Plant Proteins; D030821:Plants, Genetically Modified; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000235:genetics* / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics*
D029681:Arabidopsis Proteins; D010940:Plant Proteins; D018749:RNA, Plant; C103792:PK12 protein, Nicotiana tabacum; D011505:Protein-Tyrosine Kinases; D017346:Protein Serine-Threonine Kinases