How reliable are experimental protein-protein interaction data?
Data of protein-protein interactions provide valuable insight into the molecular networks underlying a living cell. However, their accuracy is often questioned, calling for a rigorous assessment of their reliability. The computation offered here provides an intelligible mean to assess directly the rate of true positives in a data set of experimentally determined interacting protein pairs. We show that the reliability of high-throughput yeast two-hybrid assays is about 50%, and that the size of the yeast interactome is estimated to be 10,000-16,600 interactions.
Sprinzak
Einat
E
Department of Molecular Genetics and Biotechnology, Faculty of Medicine, P.O. Box 12272, The Hebrew University of Jerusalem, 91120, Jerusalem, Israel.
Sattath
Shmuel
S
Margalit
Hanah
H
D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*
D011506:Proteins