Expression pattern of cisplatin-induced metallothionein isoforms in squamous cell carcinoma.
Cisplatin (CDDP) is a useful drug for the treatment of malignant solid tumors of the head and neck. Because CDDP includes the heavy metal platinum as a component, it is thought metallothionein (MT) may be involved in CDDP-resistance. However, functional differences between the four MT isoforms (MT-I, II, III and IV) remain unclear. The aim of this study was to investigate the relationship between MT isoform expression and CDDP-resistance. Two human tongue squamous cell carcinoma cell lines not exposed to anticancer chemotherapy were studied. The cell lines were subjected to reverse transcriptase-polymerase chain reaction (RT-PCR) analysis before and after CDDP-treatment. Both cell lines expressed MT-I/II and MT-IV isoforms but not the MT-III isoform. Following CDDP treatment, MT-I/II mRNA levels were induced only in the CDDP-resistant cell line. Our results showed that expression of the MT I/II isoform was induced by CDDP treatment, and may play an important role in CDDP-resistance in squamous cell carcinoma of the human tongue.
Nakano
Makoto
M
Department of Oral and Maxillofacial Surgery I, Okayama University Hospital of Dentistry, 2-5-1 Shikata-Cho, Okayama City, Okayama 700-8525, Japan. nakano@md.okayama-u.ac.jp
Sogawa
Chiharu Aoki
CA
Sogawa
Norio
N
Mishima
Katsuaki
K
Yamachika
Eiki
E
Mizukawa
Nobuyoshi
N
Fukunaga
Joji
J
Kawamoto
Tomoaki
T
Sawaki
Koichi
K
Sugahara
Toshio
T
Furuta
Hiroaki
H
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism*; D002945:Cisplatin / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D008668:Metallothionein / Q000096:biosynthesis* / Q000235:genetics; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014062:Tongue Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D020033:Protein Isoforms; D012333:RNA, Messenger; D008668:Metallothionein; D002945:Cisplatin