Zinc fingers and a green thumb: manipulating gene expression in plants.
Artificial transcription factors can be rapidly constructed from predefined zinc-finger modules to regulate virtually any gene. Stable, heritable up- and downregulation of endogenous genes has been demonstrated in transgenic plants. These advances promise new approaches for creating functional knockouts and conditional overexpression, and for other gene discovery and manipulation applications in plants.
Segal
David J
DJ
The Skaggs Institute for Chemical Biology and the Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA.
Stege
Justin T
JT
Barbas
Carlos F
CF
D018506:Gene Expression Regulation, Plant; D005818:Genetic Engineering / Q000379:methods*; D010944:Plants / Q000235:genetics*; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism; D016335:Zinc Fingers
D014157:Transcription Factors