Neutralizing test of hemagglutinating encephalomyelitis virus (HEV) in FS-L3 cells cultured without serum.
FS-L3 cells, originating from porcine kidney, were used for propagation of Hemagglutinating encephalomyelitis virus (HEV) and development of a virus neutralizing (VN) test. Sera of pigs, rats, cows and dogs had VN activities to HEV. On the other hand, sera of mice, rabbits, goats, sheep, horses, cats, chickens, hamsters and human did not have measurable VN activities, although these sera had high HI activities. Our results support the idea that the VN is a more reliable measure of HEV infection than the conventionally used HI test.
Sasaki
Ikuko
I
Veterinary Microbiology, Department of Veterinary Medicine, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Kazusa
Yoshinori
Y
Shirai
Junsuke
J
Taniguchi
Takahide
T
Honda
Eiichi
E
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D003332:Coronaviridae / Q000302:isolation & purification*; D003333:Coronaviridae Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology*; D016895:Culture Media, Serum-Free; D006386:Hemagglutination Tests / Q000379:methods; D007564:Japan; D007668:Kidney / Q000166:cytology / Q000821:virology; D009500:Neutralization Tests / Q000379:methods*; D013552:Swine
D016895:Culture Media, Serum-Free