[Zymohydrolysis with chondroitinase ABC and high performance liquid chromatography used for the determination of hyaluronic acid in shark fin].
A method for the determination of hyaluronic acid in shark fin by high performance liquid chromatography (HPLC) is described. At 37 degrees C, with 0.2 mol/L Tris-HCl buffer solution, hyaluronic acid was converted to the hyaluronic acid disaccharide by zymohydrolysis with chondroitinase ABC. The chromatographic conditions were as follows: ZORBAX carbohydrate analysis column (4.6 mm i.d. x 250 mm, 5 microns); room temperature; UV-VIS detector set at 226 nm; mobile phase V(acetonitrile): V(0.5% phosphoric acid) = 2:98; 0.45 micron filter membrane, pumping filter; injection volume 10 microL; flow rate 1 mL/min. The calibration curve for the hyaluronic acid disaccharide was linear over the range of 25 g/L-600 g/L. This method was applied to the analysis of shark fin with satisfactory results. The hyaluronic acid contents in different shark fins were from 0.86% to 1.96%.
Bao
Lun-jun
LJ
Guangzhou Exit-Entry Inspection and Quarantine Bureau, Guangzhou 510623, China. ljbao98@163.net
Yang
Jian-cheng
JC
He
Zhen-hua
ZH
Yang
Xiao-yun
XY
Liang
Wei-da
WD
D000818:Animals; D002138:Calibration; D019765:Chondroitin ABC Lyase; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006820:Hyaluronic Acid / Q000032:analysis*; D006868:Hydrolysis; D012754:Sharks
D006820:Hyaluronic Acid; D019765:Chondroitin ABC Lyase