Identification of contaminating adenovirus type 1 in the ATCC reference strain of respiratory syncytial virus A2 (VR-1302).
ATCC reference strain of respiratory syncytial virus A-2 (VR-1302), which was originally isolated from the lower respiratory tract of an infant (Lewis et al., 'Med. J. Aust. 2 (1961) 932'), is contaminated with adenovirus type 1. The presence of adenovirus was deduced from microscopic observation of CPE in HEp-2 cells and confirmed by electron microscopy, PCR, serological typing and immunofluoresence. Since RSV A2 is used worldwide as a representative virus of RSV type A viruses, and because the ATCC is often cited as the source of the parent stock, we considered it important to bring these findings to the attention of the wider community.
Cameron
Rachel
R
Department of Virology, Biota Holdings Limited, Level 4, 616 St Kilda Road, Melbourne 3004, Vic., Australia. rachel.cameron@med.monash.edu.au
Buck
Charles
C
Merrill
Denise
D
Luttick
Angela
A
D000260:Adenoviruses, Human / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D004866:Equipment Contamination; D006801:Humans; D007231:Infant, Newborn; D008854:Microscopy, Electron; D016133:Polymerase Chain Reaction; D012367:RNA, Viral; D012015:Reference Standards; D018113:Respiratory Syncytial Virus, Human / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D012703:Serotyping; D010948:Viral Plaque Assay
D012367:RNA, Viral