title: Effect of sample preparation, length of time, and sample size on quantification of total lipids from bovine liver.
abstract: The objective was to evaluate the effect of sample preparation (pulverization under liquid nitrogen, homogenization, or sonication), time length of sonication (0-60 s), shaking in chloroform/methanol solvent (0, 2, 4, or 12 h), incubation in chloroform (0 or 12 h), and drying of extracted lipids at 50 degrees C (2, 4, 6, or 24 h), and sample size (50-250 mg) on quantification of total lipids from bovine liver. Pulverization under liquid nitrogen yielded the lowest recovery. Sonication was least time-consuming for sample preparation. Precise estimates and the greatest recovery were obtained with 30 s of sonication, at least 2 h of shaking in chloroform/methanol solvent, 12 h of incubation in chloroform, and at least 6 h of drying. Sample sizes of at least 150 mg gave precise estimates. The results demonstrate that sample preparation, time length of different steps of the extraction procedure, and sample size affect quantification of total lipid from bovine liver.
authors.affiliation: Department of Animal Science, Iowa State University, Ames, Iowa 50011-3150, USA.
authors.forename: Burim N
authors.initials: BN
authors.lastname: Ametaj
authors.forename: Gerd
authors.initials: G
authors.lastname: Bobe
authors.forename: Yun
authors.initials: Y
authors.lastname: Lu
authors.forename: Jerry W
authors.initials: JW
authors.lastname: Young
authors.forename: Donald C
authors.initials: DC
authors.lastname: Beitz
mesh_terms: D000818:Animals; D002417:Cattle; D002725:Chloroform; D007202:Indicators and Reagents; D008055:Lipids / Q000032:analysis*; D008099:Liver / Q000737:chemistry*; D000432:Methanol; D009584:Nitrogen; D012680:Sensitivity and Specificity; D013010:Sonication; D013048:Specimen Handling / Q000379:methods*; D013997:Time Factors
chemical_list: D007202:Indicators and Reagents; D008055:Lipids; D002725:Chloroform; D009584:Nitrogen; D000432:Methanol