title: Assembly of iron-sulfur clusters mediated by cysteine desulfurases, IscS, CsdB and CSD, from Escherichia coli.
abstract: Cysteine desulfurase plays a principal role in the assembly of iron-sulfur clusters by mobilizing the sulfur atom of L-cysteine. The active site cysteine residue of the enzyme attacks the sulfur atom of L-cysteine to form a cysteine persulfide residue, and the substrate-derived sulfur atom of this residue is incorporated into iron-sulfur clusters. Escherichia coli has three cysteine desulfurases named IscS, CsdB and CSD. We found that each of them facilitates the formation of the iron-sulfur cluster of ferredoxin in vitro. Since IscU, an iron-sulfur protein of E. coli, is believed to function as a scaffold for the cluster assembly in vivo, we examined whether IscS, CsdB and CSD interact with IscU to deliver the sulfur atom to IscU. By surface plasmon resonance analysis, we found that only IscS interacts with IscU. We isolated the IscS/IscU complex, determined the residues involved in the formation of the complex, and obtained data suggesting that the sulfur transfer from IscS to IscU is initiated by the attack of Cys63 of IscU on the S gamma atom of the cysteine persulfide residue transiently produced on IscS.
authors.affiliation: Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan.
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Kurihara
authors.forename: Hisaaki
authors.initials: H
authors.lastname: Mihara
authors.forename: Shin-ichiro
authors.initials: S
authors.lastname: Kato
authors.forename: Tohru
authors.initials: T
authors.lastname: Yoshimura
authors.forename: Nobuyoshi
authors.initials: N
authors.lastname: Esaki
mesh_terms: D013437:Carbon-Sulfur Lyases / Q000737:chemistry*; D004220:Disulfides / Q000737:chemistry; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000737:chemistry*; D007506:Iron-Sulfur Proteins / Q000737:chemistry*; D008190:Lyases / Q000737:chemistry*
chemical_list: D004220:Disulfides; D029968:Escherichia coli Proteins; D007506:Iron-Sulfur Proteins; D008190:Lyases; D013437:Carbon-Sulfur Lyases; C080546:cysteine desulfurase; C034278:selenocysteine lyase