Usefulness of RA and RO isoforms of common leukocyte antigen (CD45) for early distinction between normal and abnormal promyelocytes.
Normal promyelocytes express CD45RO, while acute promyelocytic leukemia (APL) blasts express CD45RA, both isoforms of common leukocyte antigen with mutually exclusive expression. Here we report a patient with accumulation of promyelocytes in the bone marrow and the diagnostic strategy is described along with the ability to quickly discriminate between normal and abnormal cells using the flow cytometric detection of expression of RA/RO isoforms of CD45.
D'Arena
Giovanni
G
Department of Onco-Hematology, San Giovanni di Dio e Ruggi d'Aragona Hospital, 84100 Salerno, Italy. gdarena@altavista.it
Cascavilla
Nicola
N
Nunziata
Giuseppe
G
Perla
Gianni
G
Matera
Rosella
R
Carella
Angelo Michele
AM
Pistolese
Giuseppe
G
D001854:Bone Marrow Cells / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D042381:Granulocyte Precursor Cells / Q000378:metabolism*; D006801:Humans; D016130:Immunophenotyping; D015473:Leukemia, Promyelocytic, Acute / Q000175:diagnosis* / Q000378:metabolism*; D017493:Leukocyte Common Antigens / Q000737:chemistry*; D008875:Middle Aged; D020033:Protein Isoforms
D020033:Protein Isoforms; D017493:Leukocyte Common Antigens