Antioxidant properties of various solvent extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (Moringa oleifera Lam.) leaves.
Water, aqueous methanol, and aqueous ethanol extracts of freeze-dried leaves of Moringa oleifera Lam. from different agroclimatic regions were examined for radical scavenging capacities and antioxidant activities. All leaf extracts were capable of scavenging peroxyl and superoxyl radicals. Similar scavenging activities for different solvent extracts of each collection were found for the stable 1,1-diphenyl 2-picrylhydrazyl (DPPH(*)) radical. Among the three different moringa samples, both methanol and ethanol extracts of Indian origins showed the highest antioxidant activities, 65.1 and 66.8%, respectively, in the beta-carotene-linoleic acid system. Nonetheless, increasing concentration of all the extracts had significantly (P < 0.05) increased reducing power, which may in part be responsible for their antioxidant activity. The major bioactive compounds of phenolics were found to be flavonoid groups such as quercetin and kaempferol. On the basis of the results obtained, moringa leaves are found to be a potential source of natural antioxidants due to their marked antioxidant activity. This is the first report on the antioxidant properties of the extracts from freeze-dried moringa leaves. Overall, both methanol (80%) and ethanol (70%) were found to be the best solvents for the extraction of antioxidant compounds from moringa leaves.
Siddhuraju
Perumal
P
Department of Aquaculture Systems and Animal Nutrition, Institute for Animal Production in the Tropics and Subtropics, Universität Hohenheim (480b), D-70593 Stuttgart, Germany.
Becker
Klaus
K
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000975:Antioxidants / Q000032:analysis* / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000032:analysis / Q000494:pharmacology; D001713:Biphenyl Compounds; D002851:Chromatography, High Pressure Liquid; D005290:Ferric Compounds / Q000494:pharmacology; D005419:Flavonoids / Q000032:analysis; D016166:Free Radical Scavengers; D006861:Hydrogen Peroxide / Q000494:pharmacology; D019787:Linoleic Acid / Q000737:chemistry; D015227:Lipid Peroxidation / Q000187:drug effects; D029588:Moringa oleifera / Q000737:chemistry*; D009249:NADP / Q000494:pharmacology; D010084:Oxidation-Reduction; D010636:Phenols / Q000032:analysis* / Q000494:pharmacology*; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D019163:Reducing Agents; D012997:Solvents; D013481:Superoxides / Q000737:chemistry; D019207:beta Carotene / Q000737:chemistry
D000975:Antioxidants; D001713:Biphenyl Compounds; D005290:Ferric Compounds; D005419:Flavonoids; D016166:Free Radical Scavengers; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D019163:Reducing Agents; D012997:Solvents; D019207:beta Carotene; D013481:Superoxides; D009249:NADP; D000244:Adenosine Diphosphate; D019787:Linoleic Acid; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D001205:Ascorbic Acid