Expression of murine N-MYC by insertion of retrovirus sequences in a murine macrophage cell line (RAW264).
RAW264 cells, reported to be originated from Abelson-virus-induced B lymphomas, are widely used as a murine monocyte cell line. We found that RAW264 show enhanced expression of murine N-MYC. Murine cDNA clones associated with N-MYC were separated from (lambda)gt11 cDNA library constructed by using mRNA from the macrophage cell line, RAW264 cells. Sequencing analysis of the longest cDNA clone N-MYCL showed that the length of the coding region was 18 bases shorter than that of the predicted full length N-MYC cDNA, and the 3' untranslated region had the 5' long terminal repeat (LTR) sequence of the Moloney-like proviral sequence, suggesting the expression of N-MYC by insertion of the proviral sequence. This suggests that expression of N-MYC plays a role in the establishment of macrophage cell line RAW264. Integration of LTR and overexpression of the N-MYC gene might have existed in the parental lymphoma cells, playing a role in the development of lymphoma or in the establishment of macrophage cell line.
Liu
Shu-Ying
SY
Department of Pathology, Oita Medical University, Oita, Japan.
Higuchi
Yasunori
Y
Setoguchi
Mihoko
M
Matsuura
Keiko
K
Hijiya
Naoki
N
Yamamoto
Shunsuke
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D015870:Gene Expression; D015723:Gene Library; D016259:Genes, myc; D008264:Macrophages / Q000166:cytology / Q000378:metabolism*; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D008979:Moloney murine leukemia virus / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D012091:Repetitive Sequences, Nucleic Acid; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D004247:DNA