title: The ethanol oxidation system and its regulation in Pseudomonas aeruginosa.
abstract: Pseudomonas aeruginosa ATCC 17933, when growing on ethanol, uses a pyrroloquinoline quinone (PQQ)-dependent ethanol oxidation system. The genes coding for the ethanol oxidizing enzyme, a quinoprotein ethanol dehydrogenase (QEDH), cytochrome c(550), which is an essential component of the electron transport chain and accepts the electrons from QEDH, and an NAD-dependent acetaldehyde dehydrogenase form the exaABC gene cluster. Downstream of the exaBC genes the pqqABCDE gene cluster is found, which codes for proteins essential for biosynthesis of the cofactor PQQ. Also essential for growth on ethanol are an acetyl-CoA synthetase encoded by the acsA gene and a malate:quinone oxidoreductase encoded by the mqo gene. The X-ray structure of the soluble QEDH from P. aeruginosa was solved. It is a homodimeric enzyme and, aside from differences in some loops, the folding of QEDH is very similar to the large subunit of the soluble methanol dehydrogenase of methylotrophs, and the PQQ domain of the quinohemoprotein alcohol dehydrogenase from Comamonas testosteroni and P. fluorescens. Transcription from the QEDH (exaA) promoter is regulated by a two component system: a histidine sensor kinase (ExaD), which is presumably located in the cytoplasm, and a response regulator (ExaE). The phenotypic characterization and transcription studies with six regulatory mutants indicate that seven different genes in an hierarchical organization may be involved in regulating the transcription of the ethanol oxidation system and components of acetate metabolism in P. aeruginosa.
authors.affiliation: Fachgebiet Technische Biochemie, Institut für Biotechnologie der Technischen Universität Berlin, Seestrasse 13, D-13353 Berlin, Germany. Goerisch@lb.TU-Berlin.de
authors.forename: Helmut
authors.initials: H
authors.lastname: Görisch
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry; D004579:Electron Transport; D000431:Ethanol / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005810:Multigene Family; D009154:Mutation; D010084:Oxidation-Reduction; D045542:PQQ Cofactor; D011401:Promoter Regions, Genetic; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D015363:Quinolones / Q000378:metabolism; D011809:Quinones / Q000378:metabolism
chemical_list: D015363:Quinolones; D011809:Quinones; D000431:Ethanol; D045542:PQQ Cofactor; D000429:Alcohol Oxidoreductases; C019641:alcohol dehydrogenase (acceptor)