title: Effect of Phlomis umbrosa root on mast cell-dependent immediate-type allergic reactions by anal therapy.
abstract: The effect of aqueous extract of Phlomis umbrosa Turcz. (Labiatae) root (PUAE) on mast cell-dependent immediate-type allergic reaction by anal therapy was investigated. PUAE (0.01 to 1 g/kg) dose-dependently inhibited systemic anaphylaxis induced by compound 48/80 in mice. When PUAE was pretreated at the same concentrations with systemic anaphylaxis, the plasma histamine levels were reduced in a dose-dependent manner. PUAE (0.1 and 1 g/kg) also significantly inhibited local anaphylaxis activated by anti-DNP IgE. PUAE (0.001 to 1 mg/mL) dose-dependently inhibited the histamine release from rat peritoneal mast cells (RPMC) activated by compound 48/80 or anti-DNP IgE. The level of cyclic AMP (cAMP) in human mast cells (HMC-1 cells) when PUAE (1 mg/mL) was added, transiently and significantly increased compared with that of basal cells. In addition, PUAE (0.1 and 1 mg/mL) inhibited the secretion of tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-6 in phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore A23187-stimulated HMC-1 cells. These results provide evidence that anal therapy of PUAE may be beneficial in the treatment of allergic diseases.
authors.affiliation: College of Pharmacy, Woosuk University, Chonju, Chonbuk, Korea. tyshin@core.woosuk.ac.kr
authors.forename: Tae-Yong
authors.initials: TY
authors.lastname: Shin
authors.forename: Jae-Kwan
authors.initials: JK
authors.lastname: Lee
mesh_terms: D000285:Administration, Rectal; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D004136:Dinitrobenzenes / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D006636:Histamine Release / Q000187:drug effects*; D006969:Hypersensitivity, Immediate / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology; D007073:Immunoglobulin E / Q000009:adverse effects / Q000276:immunology; D015850:Interleukin-6 / Q000378:metabolism; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D010537:Peritoneum / Q000187:drug effects; D031345:Phlomis; D018517:Plant Roots; D051381:Rats; D017207:Rats, Sprague-Dawley; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D003189:p-Methoxy-N-methylphenethylamine
chemical_list: D004136:Dinitrobenzenes; D004365:Drugs, Chinese Herbal; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D007073:Immunoglobulin E; D000001:Calcimycin; D003189:p-Methoxy-N-methylphenethylamine; D000242:Cyclic AMP; D013755:Tetradecanoylphorbol Acetate