Regulation of cytokine mRNAs by interferon and interferon inducers.
Cytokine mRNA expression was studied in human long-term cell cultures of different origin: J-96 and J-41 (monocytic leukemia), SW-13 (paradrenal adenocarcinoma), and MT-4 (T-cell leukemia), in response to IFN-alpha and IFN inducers (kagocel and cycloferon). Cytokine mRNA level in the cell cultures was measured by the RT-PCR method using 11 primer pairs for the following cytokines: IFN-alpha, IFN-gamma, IL-1beta, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-18 and TNF-alpha. It was shown that IFN-alpha and IFN inducers possess an ability to regulate different cytokine mRNAs. Treatment of the cells with IFN-alpha resulted in expression of mRNAs for IL-2, IL-4, and IL-8. Kagocel induced production of IFN-alpha, IFN-gamma, and IL-2 mRNAs, and cycloferon--IFN-gamma, IL-2, IL-4, and IL-8 mRNAs. It is suggested that antiviral effects of these inducers, in general, can be attributed to imitation of cytokine responses observed in viral infection and, as a result, can lead to starting-up of cellular defense antiviral mechanisms even before action of viruses. Conclusion is made that IFN and IFN inducers may act as regulators of cytokine activity.
Vershinina
Maria Yu
MY
N.F. Gamaleya Institute for Epidemiology and Microbiology, Russian Academy of Medical Sciences, Gamaleya str. 18, Moscow 123098, Russia. narovl@mail.infotel.ru
Narovlyansky
Alexander N
AN
Deryabin
Pietr G
PG
Amchenkova
Aleftina M
AM
Ivanova
Alla M
AM
Scherbenko
Vakhtang E
VE
Nagurskaya
Elena V
EV
Bechalo
Vladimir A
VA
Timofeeva
Tatiana Yu
TY
Sanin
Alexander V
AV
Ershov
Felix I
FI
D000166:Acridines / Q000494:pharmacology; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics*; D006072:Gossypol / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D007369:Interferon Inducers / Q000378:metabolism* / Q000494:pharmacology; D007372:Interferons / Q000378:metabolism* / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000187:drug effects / Q000235:genetics
D000166:Acridines; D016207:Cytokines; D007369:Interferon Inducers; D012333:RNA, Messenger; C056991:cagocel-1; D007372:Interferons; D006072:Gossypol; C026430:10-carboxymethyl-9-acridanone