title: Molecular detection of Histoplasma capsulatum var. capsulatum in human clinical samples.
abstract: We developed a seminested PCR for the diagnosis of histoplasmosis that amplifies a portion of the Histoplasma capsulatum H antigen gene. This assay is highly sensitive and specific, being able to detect genomic material corresponding to less than 10 yeast cells without cross-reaction against other bacterial or fungal pathogens.
authors.affiliation: Instituto de Biología Molecular y Celular de Rosario, IBR-CONICET, Facultad de Ciencias Bioquímicas y Farmacéuticas, 2000 Rosario, Argentina.
authors.forename: Andrea
authors.initials: A
authors.lastname: Bracca
authors.forename: María Elena
authors.initials: ME
authors.lastname: Tosello
authors.forename: Javier E
authors.initials: JE
authors.lastname: Girardini
authors.forename: Susana L
authors.initials: SL
authors.lastname: Amigot
authors.forename: Carlos
authors.initials: C
authors.lastname: Gomez
authors.forename: Esteban
authors.initials: E
authors.lastname: Serra
mesh_terms: D000946:Antigens, Fungal / Q000235:genetics; D003470:Culture Media; D004271:DNA, Fungal / Q000032:analysis; D006658:Histoplasma / Q000235:genetics / Q000302:isolation & purification*; D006660:Histoplasmosis / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods
chemical_list: D000946:Antigens, Fungal; D003470:Culture Media; D004271:DNA, Fungal