Susceptibility of HVS-immortalized lymphocytic HSC-F cells to various strains and mutants of HIV/SIV.
Susceptibility of HSC-F, a cynomolgus macaque cell line immortalized by Herpesvirus saimiri, to infection with various primate immunodeficiency viruses were monitored. While NL432 clone of human immunodeficiency virus type 1 (HIV-1) did not grow at all in HSC-F cells, GH123 and GL-AN clones of HIV-2, and MA239 clone of simian immunodeficiency virus isolated from macaque monkeys (SIVMAC) did grow in these cells. In addition, NM-3 clone of a chimeric simian and human immunodeficiency virus (SHIV) grew fairly well in HSC-F cells. Mutational analyses of accessory genes of GL-AN were successfully performed in the HSC-F cells. These results have thus demonstrated the importance of this cell line for molecular biological studies on HIV/SIV.
Fujita
Mikako
M
Department of Virology, University of Tokushima Graduate School of Medicine, Japan.
Yoshida
Akiko
A
Sakurai
Akiko
A
Tatsuki
Junko
J
Ueno
Fumiko
F
Akari
Hirofumi
H
Adachi
Akio
A
D000818:Animals; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D015658:HIV Infections / Q000378:metabolism; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism*; D015498:HIV-2 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism; D016097:Simian Acquired Immunodeficiency Syndrome / Q000378:metabolism; D015302:Simian Immunodeficiency Virus / Q000235:genetics / Q000378:metabolism*