title: Histochemical, immunohistochemical, and ultrastructural studies of gingival fibromatosis: a case report.
abstract: Gingival fibromatosis is a rare disease characterized by enlargement of the gingiva. The purpose of this study was to analyze a case of idiopathic gingival fibromatosis, using histochemical and immunohistochemical staining and transmission electron microscopy. The patient was a 39-year-old Japanese man, in whom the gingiva was enlarged throughout the entire mandible and maxilla. Specimens of gingival fibromatosis exhibited epithelial hyperplasia and increased amounts of collagen fiber bundles in the connective tissue light-microscopically. Well-developed collagen bundles were strongly stained with Azan and Masson trichrome staining. Immunohistochemically, the gingival connective tissue was specifically stained by type I collagen and vimentin antibodies. Ultrastructurally, the lesion consisted of fibroblasts and mature collagen fibers running in all directions. No myofibroblasts were detected histochemically, immunohistochemically, or ultrastructurally. These findings suggested that this disease may be the result of an increase in collagen synthesis by the fibroblasts and/or that it may be associated with one of the findings of histologic heterogeneity.
authors.affiliation: The First Department of Oral and Maxillofacial Surgery, Kagoshima University Dental School, 8-35-1 Sakuragaoka, Kagoshima 890-8544, Japan.
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Sakamoto
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Nitta
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kamikawa
authors.forename: Seishi
authors.initials: S
authors.lastname: Kono
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Kamikawa
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Sugihara
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Tsuyama
authors.forename: Fusayoshi
authors.initials: F
authors.lastname: Murata
mesh_terms: D000328:Adult; D005351:Fibromatosis, Gingival / Q000473:pathology / Q000648:ultrastructure*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron