title: The first-generation whole-genome radiation hybrid map in the horse identifies conserved segments in human and mouse genomes.
abstract: A first-generation radiation hybrid (RH) map of the equine (Equus caballus) genome was assembled using 92 horse x hamster hybrid cell lines and 730 equine markers. The map is the first comprehensive framework map of the horse that (1) incorporates type I as well as type II markers, (2) integrates synteny, cytogenetic, and meiotic maps into a consensus map, and (3) provides the most detailed genome-wide information to date on the organization and comparative status of the equine genome. The 730 loci (258 type I and 472 type II) included in the final map are clustered in 101 RH groups distributed over all equine autosomes and the X chromosome. The overall marker retention frequency in the panel is approximately 21%, and the possibility of adding any new marker to the map is approximately 90%. On average, the mapped markers are distributed every 19 cR (4 Mb) of the equine genome--a significant improvement in resolution over previous maps. With 69 new FISH assignments, a total of 253 cytogenetically mapped loci physically anchor the RH map to various chromosomal segments. Synteny assignments of 39 gene loci complemented the RH mapping of 27 genes. The results added 12 new loci to the horse gene map. Lastly, comparison of the assembly of 447 equine genes (256 linearly ordered RH-mapped and additional 191 FISH-mapped) with the location of draft sequences of their human and mouse orthologs provides the most extensive horse-human and horse-mouse comparative map to date. We expect that the foundation established through this map will significantly facilitate rapid targeted expansion of the horse gene map and consequently, mapping and positional cloning of genes governing traits significant to the equine industry.
authors.affiliation: Department of Veterinary Anatomy and Public Health, College of Veterinary Medicine, Texas A&M University, College Station, Texas 77843, USA. bchowdhary@cvm.tamu.edu
authors.forename: Bhanu P
authors.initials: BP
authors.lastname: Chowdhary
authors.forename: Terje
authors.initials: T
authors.lastname: Raudsepp
authors.forename: Srinivas R
authors.initials: SR
authors.lastname: Kata
authors.forename: Glenda
authors.initials: G
authors.lastname: Goh
authors.forename: Lee V
authors.initials: LV
authors.lastname: Millon
authors.forename: Veronica
authors.initials: V
authors.lastname: Allan
authors.forename: François
authors.initials: F
authors.lastname: Piumi
authors.forename: Gérard
authors.initials: G
authors.lastname: Guérin
authors.forename: June
authors.initials: J
authors.lastname: Swinburne
authors.forename: Matthew
authors.initials: M
authors.lastname: Binns
authors.forename: Teri L
authors.initials: TL
authors.lastname: Lear
authors.forename: Jim
authors.initials: J
authors.lastname: Mickelson
authors.forename: James
authors.initials: J
authors.lastname: Murray
authors.forename: Douglas F
authors.initials: DF
authors.lastname: Antczak
authors.forename: James E
authors.initials: JE
authors.lastname: Womack
authors.forename: Loren C
authors.initials: LC
authors.lastname: Skow
mesh_terms: D000818:Animals; D002460:Cell Line; D017124:Conserved Sequence / Q000235:genetics*; D006224:Cricetinae; D005819:Genetic Markers / Q000235:genetics / Q000528:radiation effects; D016678:Genome; D015894:Genome, Human; D006736:Horses / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence / Q000379:methods / Q000706:statistics & numerical data / Q000662:veterinary; D051379:Mice; D018895:Microsatellite Repeats / Q000235:genetics / Q000528:radiation effects; D008969:Molecular Sequence Data; D021101:Radiation Hybrid Mapping / Q000379:methods* / Q000706:statistics & numerical data / Q000662:veterinary*; D016415:Sequence Alignment / Q000379:methods / Q000706:statistics & numerical data / Q000662:veterinary; D016008:Statistical Distributions; D026801:Synteny / Q000235:genetics / Q000528:radiation effects
chemical_list: D005819:Genetic Markers