Pulsatile and moderated release of dalareline from Zn(II) complexes in the form of suspension.
The influence of molar relation of the Zn(II)-Dalareline upon the speed and character of peptide release from these complexes was defined. Sparingly soluble complexes were obtained in the form of suspension to be applied palanterally. The dynamics of peptide release was observed in vitro conditions. The results obtained show that dalareline is released from all the complexes in the pulsatile way. 5 to 7 significant peaks of the released peptide were observed. It was stated that the molar relation of Zn(II)-Dalareline in the range of 0.5:1 do 100:1 ensures pulsatile character of peptide release from all the complexes. Depending upon this relation dalareline release is differentiated, and the peaks occur in different times. The number of peaks in this case is not connected with the molar relation of Zn(II)-Dalareline. However, it is significantly connected with the amplitude and the size of peaks of the released peptide. The time of half-release of dalareline was 3.12 h to 26.45 h and it was stated that it is significantly dependent upon the molar relation of Zn(II)-Dalareline. The greater the molar relation of the Zn(II)-Dalareline, the longer the time of the peptide release from this complex (r = 0.518).
Ryszka
F
F
Department of Applied Pharmacy and Drug Technology, Silesian Medical Academy Sosnowiec, ul. Kasztanowa 3, Poland.
Dolińska
B
B
D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives / Q000737:chemistry*; D007700:Kinetics; D009942:Organometallic Compounds / Q000737:chemistry*; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet; D013535:Suspensions; D015032:Zinc
C082465:LHRH ethylamide, des-Gly(10)-Ala(6)-; D009942:Organometallic Compounds; D013535:Suspensions; D007987:Gonadotropin-Releasing Hormone; D015032:Zinc