title: Paracrine effects of PAMP and adrenomedullin on the human adrenal H295R cell line: PAMP but not adrenomedullin stimulates DHEA secretion.
abstract: It has previously been shown, by this laboratory and others, that adrenal cells actively secrete adrenomedullin. Here it is demonstrated that human adrenal cells also secrete the related peptide, proadrenomedullin N-terminal 20 peptide (PAMP). The actions of adrenomedullin and PAMP on adrenal steroid secretion were determined by measuring the aldosterone, cortisol and dehydroepiandrosterone (DHEA) content of cell culture medium after exposure of the human adrenal H295R cells to either PAMP or adrenomedullin. While PAMP was found to cause a dose-dependent increase in release of all the steroids into the medium, adrenomedullin only increased aldosterone and cortisol and had no effect on DHEA. These data suggest that both adrenomedullin and PAMP may be autocrine regulators of adrenal steroid secretion.
authors.affiliation: Department of Endocrinology, Barts and The London, Queen Mary School of Medicine and Dentistry, University of London, Suite 12, Dominion House, Bartholomew Close, EC1A 7BE, London, UK.
authors.forename: Laura M
authors.initials: LM
authors.lastname: Thomson
authors.forename: Supriya
authors.initials: S
authors.lastname: Kapas
authors.forename: Joy P
authors.initials: JP
authors.lastname: Hinson
mesh_terms: D000302:Adrenal Cortex / Q000187:drug effects / Q000378:metabolism*; D053607:Adrenomedullin; D000450:Aldosterone / Q000096:biosynthesis; D002460:Cell Line; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000096:biosynthesis; D003687:Dehydroepiandrosterone / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D019899:Paracrine Communication; D010455:Peptides / Q000494:pharmacology*; D011498:Protein Precursors / Q000494:pharmacology*; D011506:Proteins / Q000494:pharmacology*
chemical_list: D010455:Peptides; D011498:Protein Precursors; D011506:Proteins; C088633:proadrenomedullin; D053607:Adrenomedullin; D005576:Colforsin; D003687:Dehydroepiandrosterone; D000450:Aldosterone; D000242:Cyclic AMP