title: Expression of the antiapoptotic gene survivin in chronic myeloid leukemia.
abstract: Survivin, a unique member of the inhibitor of the apoptosis protein (IAPs) family, is over-expressed in many cancers but not in normal differentiated adult tissues. Using semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR), we investigated patterns of survivin gene expression in a group of 12 patients with chronic myeloid leukemia (CML) representing both chronic and blastic phases of the disease. All 6 patients in chronic phase CML were uniformly negative for the survivin transcript, in contrast to 4 Philadelphia chromosome-positive (Ph+) CML patients in blastic crisis, all of whom (100%) were positive for survivin with tangible levels of expression. However, survivin expression was markedly down-regulated in 2 atypical CML patients with Philadelphia chromosome-negative (Ph-) blastic crisis. Our data indicates that up-regulation of survivin expression may be involved in typical CML evolution from the chronic into the blastic phase of the disease.
authors.affiliation: First Department of Internal Medicine, Fukui Medical University, Matsuoka, Fukui, 910-1193, Japan.
authors.forename: Adel
authors.initials: A
authors.lastname: Badran
authors.forename: Akira
authors.initials: A
authors.lastname: Yoshida
authors.forename: Yuji
authors.initials: Y
authors.lastname: Wano
authors.forename: Shin
authors.initials: S
authors.lastname: Imamura
authors.forename: Yasukazu
authors.initials: Y
authors.lastname: Kawai
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tsutani
authors.forename: Manabu
authors.initials: M
authors.lastname: Inuzuka
authors.forename: Takanori
authors.initials: T
authors.lastname: Ueda
mesh_terms: D017209:Apoptosis / Q000235:genetics; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D018922:HL-60 Cells; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis* / Q000235:genetics; D009363:Neoplasm Proteins; D010677:Philadelphia Chromosome; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000077022:Survivin
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D000077022:Survivin