Laccase-catalysed synthesis of coupling products of phenolic substrates in different reactors.
Substrate oxidation of aromatic substances by the enzyme laccase followed by a heteromolecular coupling with a co-substrate is a promising possibility for the synthesis of new compounds. To find a suitable reactor for the effective production of new compounds, the laccase-catalysed coupling of 3-(3,4-dihydroxyphenyl)propionic acid with 4-aminobenzoic acid was investigated as a model system. Based on the kinetic parameters, a mathematical model was used to predict the reaction yield and oxygen demand in a discontinuously stirred tank reactor and a continuously operated stirred tank reactor. Membrane processes were used for bubble-free aeration of the system and to recover the soluble enzyme.
Pilz
R
R
Rostock University, Department of Chemistry, 18051 Rostock, Germany.
Hammer
E
E
Schauer
F
F
Kragl
U
U
D010129:4-Aminobenzoic Acid / Q000378:metabolism; D019149:Bioreactors; D002109:Caffeic Acids / Q000378:metabolism; D003198:Computer Simulation; D005656:Fungal Proteins / Q000378:metabolism*; D007700:Kinetics; D042845:Laccase; D008956:Models, Chemical; D010088:Oxidoreductases / Q000378:metabolism*; D010100:Oxygen / Q000494:pharmacology; D011124:Polyporaceae / Q000201:enzymology; D013379:Substrate Specificity
D002109:Caffeic Acids; D005656:Fungal Proteins; C000995:3,4-dihydroxyphenylpropionic acid; D010088:Oxidoreductases; D042845:Laccase; D010100:Oxygen; D010129:4-Aminobenzoic Acid