Modulation of Basophil Histamine Release and T Cell Reactivity by Chemically Modified Allergen.
Modification of a model allergen ovalbumin (OA) with succinylation (Suc-OA) led to inhibition of allergen histamine releasing activity as it was tested on basophils of OA-sensitive patients. A whole blood leukocyte histamine release was performed by glass fibre based histamine assay. IgE-binding activity of Suc-OA was significantly reduced as it was shown by RAST inhibition technique. Suc-OA stimulated OA-specific T cell hybrid 3DO-548 and ACP:LK35 to produce cytokine release at the same level as in the case of non-modified OA. Succinylation of OA was concluded to result in selective blockade of B cell and preservation of T cell epitopes of the allergen suggesting a new approach for allergen specific immunotherapy.
Gushchin
I. S.
IS
Institute of Immunology, Moscow, Russia.
Babakhin
A. A.
AA
Andreev
S. M.
SM
Wheeler
A. W.
AW
Stockinger
B.
B
Nolte
H.
H
DuBuske
L. M.
LM