[The study on the stable inheritance and expression of foreign gene in transgenic mice].
Two transgenic mouse strains, in which the expression of human factor IX (hFIX) in the milk were different significantly, were bred, and the foreign gene integration as well as the content of hFIX in the milk were detected by PCR, Southern blot, FISH and ELISA, respectively. The results showed that approximately 50% offsprings were transgenic positive. Foreign gene integrated in mouse chromosomes was intact. The hFIX expression of each mouse in the same strain was different, the content of hFIX in the milk was (43.32 +/- 5.41) microgram/mL in FIX-33 transgenic strain and (1.16 +/- 0.45) microgram/mL in FIX-124 transgenic strain. Meanwhile, the hFIX gene expression between the two strains was different remarkably (P < 0.01). We conclude that the characteristics of inheritance and expression in the founder were able to be transferred to their offsprings stably.
Yan
Jing-Bin
JB
Shanghai Institute of Medical Genetics, Shanghai Children's Hospital, Shanghai 200040, China. ytzeng@stn.sh.cn or yanjingbin@sina.com
Xiao
Yan-Ping
YP
Fang
Yu-Dan
YD
Xi
Ying
Y
Huang
Wen-Ying
WY
Huang
Ying
Y
D000818:Animals; D005164:Factor IX / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008822:Mice, Transgenic; D008892:Milk / Q000737:chemistry; D011994:Recombinant Proteins / Q000096:biosynthesis*
D011994:Recombinant Proteins; D005164:Factor IX