[The effect of metallothionein on delaying protection of ischemia/reperfusion].
OBJECTIVE
To study the possibility of metallothionein (MT) participating in the process of ischemia/reperfusion protection.


METHODS
The model of pig latissimus dorsi island flap and cultured myocytes were used in this study. The island flap was preconditioned with ischemia and the cultured myocytes were treated with anoxia. MT was detected in the flap and the cultured myocytes instantly and at 12 and 24 hours after the treatment. The protection effect of precondition on flap ischemia/reperfusion damage was observed by recording flap necrosis and the serum LDH level. The protection effect of precondition on myocyte anoxia/reoxygenation damage was observed by recording the cell survival rate and MDA content. The change in the protection effect was also observed after the MT was inhibited by PD098059.


RESULTS
The content of MT in cultured myocytes increased obviously 12 hours after precondition. Similar result was observed in flap tissue 24 hours later. Compared with the non-conditioned flap, the necrosis areas of the preconditioned flaps were smaller, the serum LDH was lower. The survival rate of preconditioned cultured myocytes was higher compared with non-conditioned cells (P < 0.05). The content of MDA and the quantity of discharged LDH were less (P < 0.05). When MT was inhibited by PD098059, the delaying protection of precondition disappeared. The observed quotas at that time were the same with those of simple I/R or A/R groups (P > 0.05).


CONCLUSIONS
24 hours after precondition, the flap and the cultured myocytes are protected from re-damage of I/R or A/R. MT is involved in this process.
Guo
Jie
J
Department of Plastic Surgery, The Second Hospital, Harbin Medical University, Harbin 150086, China.
Jiang
Hongchi
H
Wang
Shujie
S
D000818:Animals; D015687:Cell Hypoxia / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005419:Flavonoids / Q000494:pharmacology; D019194:Ischemic Preconditioning; D007770:L-Lactate Dehydrogenase / Q000097:blood / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D008668:Metallothionein / Q000187:drug effects / Q000378:metabolism*; D009132:Muscles / Q000166:cytology / Q000378:metabolism / Q000601:surgery; D009336:Necrosis; D015427:Reperfusion Injury / Q000378:metabolism*; D013524:Surgical Flaps / Q000473:pathology; D013552:Swine; D013997:Time Factors
D004791:Enzyme Inhibitors; D005419:Flavonoids; D008315:Malondialdehyde; D008668:Metallothionein; D007770:L-Lactate Dehydrogenase; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one