[Effect of activation of cellular immunity on p58+ cells expressing killer-cell-inhibitory receptor cells].
The purpose of this study was to evaluate the effects of cellular immunity activation on P58(+) cells expressing killer cell inhibitory receptor (KIR) and their regulatory function on cellular immunity, and provid theoretical data for preventing graft-vers-host disease (GVHD) in stem cell transplantation therapy. The mononuclear cells from human peripheral blood were incubated with IL-2, Con A and Lipostin (LP) for 72 hours. The KIR expressing cells, P58.1(+) and P58.2(+) cells, were analyzed by flow cytometry. The results showed that the percentages of CD3(+), CD4(+), CD8(+), CD16(+)CD56(+), P58.1(+) and P58.2(+) cells were greatly increased after treated with IL-2, Con A and LP, separately or in combination, and the percentages of above cells in combined treatment groups were higher than those of single stimulated groups, especially the percentage of cells in the IL-2 + LP group was significantly higher than those in IL-2 and LP singly treated groups.


CONCLUSIONS
IL-2, Con A and LP possess the ability to induce the expression of KIR and stimulate proliferation of P58.1(+) and P58.2(+) cells while to activate the celluar immunity response, the expression of P58 gene may be regulated by the activation of cellular immunity.
Pang
Xing-Hua
XH
Department of Hematology, Zhujiang Hospital, The First Military Medical University, Guangzhou 510282, China. xinghuapan@yahoo.com.cn
Pang
Rong-Qing
RQ
Guo
Kun-Yuan
KY
Song
Jiu-Gang
JG
Li
Jiang-Qi
JQ
Zhang
Yu-Jin
YJ
Yang
Xiao-Fen
XF
D000328:Adult; D017252:CD3 Complex / Q000032:analysis; D015704:CD4 Antigens / Q000032:analysis; D019002:CD56 Antigen / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology; D005434:Flow Cytometry; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D017452:Receptors, IgG / Q000032:analysis; D011971:Receptors, Immunologic / Q000032:analysis*; D054340:Receptors, KIR; D054344:Receptors, KIR2DL3
D017252:CD3 Complex; D015704:CD4 Antigens; D019002:CD56 Antigen; D016827:CD8 Antigens; D007376:Interleukin-2; C515078:KIR2DL3 protein, human; D017452:Receptors, IgG; D011971:Receptors, Immunologic; D054340:Receptors, KIR; D054344:Receptors, KIR2DL3; D003208:Concanavalin A