Rapid and simple determination of selenium in blood serum by inductively coupled plasma-mass spectrometry (ICP-MS).
An inductively coupled plasma mass spectrometer (ICP-MS) with a rapid sample-preparative procedure was used for the determination of selenium in blood serum. Blood serum was prepared by dilution in an acidic solution consisting of nitric acid (1%), X-triton (0.1%) and 1-butanol (0.8%). A calibration curve was established for 1-40 microg mL(-1) (r(2)>0.99). The limit of detection was 0.5 microg mL(-1). Repeatability and intermediate precision were satisfactory with relative standard deviations (RSD) of 2.0% and 3.2%, respectively. This method was easily applied to reference materials with satisfactory accuracy. Good correlation (r(2)=0.96) was observed between ICP-MS and atomic absorption spectrometry (AAS) for the determination of (82)Se in blood serum from 23 patients. These results suggest that the sample preparative procedure coupled with ICP-MS can be used for the routine determination of (82)Se in human blood serum.
Labat
L
L
Laboratoire de Biochimie et de Biologie Moléculaire, Hôpital Calmette, Boulevard du Professeur Leclerc, 59037 Lille cedex, France. l.labat@chru-lille.fr
Dehon
B
B
Lhermitte
M
M
D001774:Blood Chemical Analysis / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D012016:Reference Values; D012643:Selenium / Q000097:blood*; D012680:Sensitivity and Specificity
D012643:Selenium