Cardiac nociception in rats: neuronal pathways and the influence of dermal neurostimulation on conveyance to the central nervous system.
Neurostimulation for refractory angina pectoris is often advocated for its clinical efficacy. However, the recruited pathways to induce electroanalgesia are partially unknown. Therefore, we sought to study the effect of neurostimulation on experimentally induced cardiac nociception, using capsaicin as nociception-induced substance. Four different groups of male Wistar rats were pericardially infused with either saline or capsaicin with or without neurostimulation. Group StimCap was infused with capsaicin, and group StimVeh was infused with saline. Both groups were treated with neurostimulation. Group ShamCap was only infused with capsaicin without stimulation, whereas group ShamVeh was only infused with saline. Neuronal activation differences were assessed with cytochemical staining, revealing the cellular expression of c-fos. Pain behavior was registered on video and was quantitatively analyzed. In the StimCap and ShamCap groups, all animals exerted typical pain behavior, whereas in the StimVeh group only moderate changes in behavior were observed. Group ShamVeh animals were unaffected by the procedure. The upper thoracic spinal cord showed high numbers of c-fos-positive cells, predominantly in laminae III and IV in both StimCap and StimVeh groups. Almost no c-fos expression was noticed in groups ShamCap and ShamVeh in these sections of the spinal cord. In groups StimCap and ShamCap a significantly higher number of c-fos-positive cells in comparison with groups StimVeh and ShamVeh were noticed in the periambigus region, the nucleus tractus solitarius, and the paraventricular hypothalamus. In the paraventricular thalamus, periaqueductal gray, and central amygdala, no significant differences were noticed among the first three groups, and the c-fos concentration in these three groups was significantly higher than in group ShamVeh. It is concluded that neurostimulation does not influence capsaicin-induced cardiac nociceptive pain pulses to the central nervous system. Furthermore, capsaicin-induced cardiac pain and neurostimulation may utilize two different pathways.
Albutaihi
Ibrahim A M
IA
Faculty of Medical Sciences, Groningen, The Netherlands.
Hautvast
Raymond W M
RW
DeJongste
Mike J L
MJ
Ter Horst
Gert J
GJ
Staal
Michiel J
MJ
D000344:Afferent Pathways / Q000187:drug effects / Q000502:physiology*; D000698:Analgesia; D000787:Angina Pectoris / Q000503:physiopathology* / Q000628:therapy; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology; D001921:Brain / Q000187:drug effects / Q000502:physiology; D002211:Capsaicin / Q000494:pharmacology; D002490:Central Nervous System / Q000187:drug effects / Q000502:physiology*; D006321:Heart / Q000294:innervation*; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D009619:Nociceptors / Q000187:drug effects / Q000502:physiology*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013133:Spinothalamic Tracts / Q000187:drug effects / Q000502:physiology; D017779:Sympathetic Fibers, Postganglionic / Q000187:drug effects / Q000502:physiology; D004561:Transcutaneous Electric Nerve Stimulation; D014630:Vagus Nerve / Q000187:drug effects / Q000502:physiology
D016760:Proto-Oncogene Proteins c-fos; D002211:Capsaicin