Immediate DNA ploidy analysis of gastrointestinal biopsies taken by endoscopy using a mechanical dissociation device.
BACKGROUND
Quantitative DNA analysis of fresh biopsy material can contribute to a more accurate diagnosis and prognosis. The authors aimed to develop and test a mechanical, nuclear preparation protocol for quantitative DNA analysis.


METHODS
Altogether 32 gastric (10 healthy, 17 gastritis, 7 adenocarcinoma) and 48 colon (21 healthy, 20 colitis ulcerosa, 7 adenocarcinoma) biopsy specimens were evaluated. The mechanical disruption was performed by Medimachine (DAKO, Denmark). The flow cytometry analysis was performed on a BD FACSStar flow cytometer.


RESULTS
DNA Aneuploidy was found in gastric samples only in tumours. The S-phase fraction of the normal cases was 5.9 +/- 2.1%, 5.1% +/- 1.2% in gastritis and 10.7 +/- 1.6% in carcinomas. Seven out of 20 colitis ulcerosa and 4 out of 7 colon cancer samples were aneuploid. The S-phase fraction of normal colon cases was 5.7 +/- 3.4%, in colitis 8.1 +/- 4.2% and 15.1 +/- 5.7% in carcinomas, respectively.


CONCLUSIONS
Mechanical nuclear isolation is a useful method for flow cytometric DNA ploidy analysis of fresh biopsy samples.
Molnar
Bela
B
Clinical Gastroenterology Unit, Second Department of Medicine, Semmelweis University of Medicine, 1088 Budapest, Hungary. mb@bel2.sote.hu
Bocsi
Jozsef
J
Karman
Jozsef
J
Nemeth
Annamaria
A
Pronai
Laszlo
L
Zagoni
Tamas
T
Tulassay
Zsolt
Z
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000782:Aneuploidy; D001706:Biopsy / Q000379:methods*; D003093:Colitis, Ulcerative / Q000235:genetics / Q000473:pathology; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology; D003113:Colonoscopy / Q000379:methods; D004273:DNA, Neoplasm / Q000032:analysis* / Q000235:genetics; D005434:Flow Cytometry; D005756:Gastritis / Q000235:genetics / Q000473:pathology; D005773:Gastroscopy / Q000379:methods; D006801:Humans; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
D004273:DNA, Neoplasm