Role of hippocampal alpha(2A)-adrenergic receptor in methamphetamine-induced hyperlocomotion in the mouse.
Psychostimulants, such as methamphetamine (METH), induce psychological dependence and we recently suggested that hippocampal alpha(2A)-adrenergic receptor (alpha(2A)-AR) is involved in METH-induced modulation of central nervous systems. The present study shows that pretreatment with yohimbine dose-dependently decreased the ambulatory hyperactivities induced by METH (2 mg/kg) in mice. Moreover, specific knock-down of the hippocampal alpha(2A)-AR with infusion of anti-sense oligo DNA of alpha(2A)-AR significantly suppressed ambulatory activity induced by METH administration. Infusion of sense oligo DNA of alpha(2A)-AR into mouse hippocampus exerted no effects on the ambulatory activity. These observations strongly suggest the involvement of hippocampal alpha(2A)-AR in the regulation of ambulatory activity induced by METH administration.
Nishio
Masahiro
M
Department of Pharmacology, National Defense Medical College, 3-2 Namiki, 359-8513, Tokorozawa, Japan.
Kuroki
Yasutomi
Y
Watanabe
Yasuhiro
Y
D018759:Adrenergic Uptake Inhibitors; D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology; D000818:Animals; D021461:Animals, Outbred Strains; D004305:Dose-Response Relationship, Drug; D004333:Drug Administration Routes; D019206:GTP-Binding Protein alpha Subunits, Gi-Go; D020962:Heterotrimeric GTP-Binding Proteins / Q000378:metabolism; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D006948:Hyperkinesis / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology; D008694:Methamphetamine; D051379:Mice; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology; D018341:Receptors, Adrenergic, alpha-2 / Q000096:biosynthesis / Q000378:metabolism*; D013997:Time Factors; D015016:Yohimbine / Q000494:pharmacology
C547769:Adra2a protein, mouse; D018759:Adrenergic Uptake Inhibitors; D000317:Adrenergic alpha-Antagonists; D020319:Oligodeoxyribonucleotides, Antisense; D018341:Receptors, Adrenergic, alpha-2; D015016:Yohimbine; D008694:Methamphetamine; D019206:GTP-Binding Protein alpha Subunits, Gi-Go; D020962:Heterotrimeric GTP-Binding Proteins