Quartz-crystal microbalance immunosensor for Schistsoma-japonicum-infected rabbit serum.
A quartz-crystal microbalance immunosensor (QCM) has been developed for the direct determination of Schistosoma-japonicum-infected rabbit serum. A self-assembled monolayer with carboxyl groups was first coated on a gold electrode of a quartz-crystal resonator by the spontaneous adsorption of 3-mercaptopropionic acid. Schistosoma-japonicum molecular antigen of 32 kD molecular weight was then covalently attached to the crystal surface. The QCM immunosensor was used to detect infected rabbit serum (IRS49-2000); a maximum titer of 1:800 was achieved.
Wu
Zhao-Yang
ZY
State Key Laboratory of Chemo/Biosensing and Chemometrics, Institute of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China. zywu@hnu.net.cn
Shen
Guo-Li
GL
Wang
Shi-Ping
SP
Yu
Ru-Qin
RQ
D015097:3-Mercaptopropionic Acid / Q000737:chemistry; D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods*; D011791:Quartz; D011817:Rabbits; D012015:Reference Standards; D012549:Schistosoma japonicum / Q000276:immunology / Q000302:isolation & purification*; D012552:Schistosomiasis / Q000097:blood* / Q000276:immunology / Q000469:parasitology*; D013997:Time Factors
D000947:Antigens, Helminth; D011791:Quartz; D015097:3-Mercaptopropionic Acid