Identification of the minor component of a mixed stain by using mismatch primer-induced restriction sites in amplified mtDNA.
We report a case in which STR typing failed to identify the minor component of a mixed saliva stain, but a mitochondrial restriction analysis succeeded in discriminating between the two components. To identify the nt16093 and nt16265 transitions, the template was amplified with the mismatch primers L16092-mm16085 and H16266-mm16269. In the presence of the transitions the mismatch primers created a BsaB I and a Cac8 I restriction site, respectively. Subsequently, aliquots were restricted separately using the enzymes Cac8 I and BsaB I which clearly identified the minor stain component.
Szibor
R
R
Institut für Rechtsmedizin, Otto-von-Guericke-Universität Magdeburg, Leipziger Strasse 44, 39120 Magdeburg, Germany. reinhard.szibor@medizin.uni-magdeburg.de
Michael
M
M
Plate
I
I
Wittig
H
H
Krause
D
D
D001628:Beverages; D003415:Crime; D019548:Crime Victims; D016172:DNA Fingerprinting / Q000379:methods; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000032:analysis* / Q000235:genetics; D006801:Humans; D008297:Male; D012463:Saliva / Q000737:chemistry*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods; D020080:Tandem Repeat Sequences
D017931:DNA Primers; D004272:DNA, Mitochondrial