title: Recent news related to substrates and inhibitors of plant amine oxidases.
abstract: Reactions of pea diamine oxidase (PSAO) and maize polyamine oxidase (MPAO) with 1,4-bis(3-aminopropyl)-piperazine (BAPP), diethylenetriamine (DETA), dipropylenetriamine (DPTA), dehydrospermine (DHSP) and 3-oxapentane-1,5-diamine (OPD) were studied and compared. These reactions were characterised by kinetic measurements (kinetic constants, stoichiometry) and by measurements of absorption spectra (reaction mechanisms). In the case of oxidised polyamine compounds, the corresponding reaction products were determined using analytical methods (coloured trapping reactions, mass spectrometry, IR spectroscopy, thin layer chromatography). Some of the compounds were found to be substrates of PSAO and relatively potent inhibitors of MPAO (and vice versa) all at once. The others showed the same effect on both enzymes. This may have an importance for designing of experiments in physiological studies in plants.
authors.affiliation: Department of Biochemistry, Faculty of Science, Palacký University, Slechtitelů 11, CZ-783 71 Olomouc-Holice, Czech Republic. sebela@risc.upol.cz
authors.forename: Marek
authors.initials: M
authors.lastname: Sebela
authors.forename: Zbynek
authors.initials: Z
authors.lastname: Lamplot
authors.forename: Marek
authors.initials: M
authors.lastname: Petrivalský
authors.forename: David
authors.initials: D
authors.lastname: Kopecný
authors.forename: Karel
authors.initials: K
authors.lastname: Lemr
authors.forename: Ivo
authors.initials: I
authors.lastname: Frébort
authors.forename: Pavel
authors.initials: P
authors.lastname: Pec
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D018532:Pisum sativum / Q000201:enzymology*; D010940:Plant Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D003313:Zea mays / Q000201:enzymology*
chemical_list: D004791:Enzyme Inhibitors; D010940:Plant Proteins; D006631:Amine Oxidase (Copper-Containing)