title: Identification of contaminating adenovirus type 1 in the ATCC reference strain of respiratory syncytial virus A2 (VR-1302).
abstract: ATCC reference strain of respiratory syncytial virus A-2 (VR-1302), which was originally isolated from the lower respiratory tract of an infant (Lewis et al., 'Med. J. Aust. 2 (1961) 932'), is contaminated with adenovirus type 1. The presence of adenovirus was deduced from microscopic observation of CPE in HEp-2 cells and confirmed by electron microscopy, PCR, serological typing and immunofluoresence. Since RSV A2 is used worldwide as a representative virus of RSV type A viruses, and because the ATCC is often cited as the source of the parent stock, we considered it important to bring these findings to the attention of the wider community.
authors.affiliation: Department of Virology, Biota Holdings Limited, Level 4, 616 St Kilda Road, Melbourne 3004, Vic., Australia. rachel.cameron@med.monash.edu.au
authors.forename: Rachel
authors.initials: R
authors.lastname: Cameron
authors.forename: Charles
authors.initials: C
authors.lastname: Buck
authors.forename: Denise
authors.initials: D
authors.lastname: Merrill
authors.forename: Angela
authors.initials: A
authors.lastname: Luttick
mesh_terms: D000260:Adenoviruses, Human / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D004866:Equipment Contamination; D006801:Humans; D007231:Infant, Newborn; D008854:Microscopy, Electron; D016133:Polymerase Chain Reaction; D012367:RNA, Viral; D012015:Reference Standards; D018113:Respiratory Syncytial Virus, Human / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D012703:Serotyping; D010948:Viral Plaque Assay
chemical_list: D012367:RNA, Viral