title: Increased platelet Fc receptor expression as a potential contributing cause of platelet hypersensitivity to collagen in diabetes mellitus.
abstract: Recent clinical and laboratory observations support a potential role for the platelet FcgammaRIIA receptor (FcR) in collagen-mediated platelet activation associated with arterial thrombosis. Stable age- and sex-independent variation in receptor expression exists. Flow cytometry showed that 100 diabetes patients had increased mean platelet FcR expression levels compared with 201 non-diabetes patients (P < 0.001). Aggregation studies following FcR cross-linking supported a receptor role in increased activation of diabetes platelets after collagen stimulation (P = 0.018). Immunoprecipitation of collagen-stimulated and FcR-crosslinked platelets demonstrated enhanced levels of tyrosine-phosphorylated Syk in diabetic platelets. Increased platelet FcR-mediated sensitivity to collagen may contribute to cardiovascular morbidity and mortality in diabetes.
authors.affiliation: Divisions of Hematology and Medical Oncology, University of Colorado Health Science Center, 4200 East 9th Avenue, Denver, CO 80262, USA. david.calverley@uchsc.edu
authors.forename: David C
authors.initials: DC
authors.lastname: Calverley
authors.forename: Michele R
authors.initials: MR
authors.lastname: Hacker
authors.forename: Kimberly A
authors.initials: KA
authors.lastname: Loda
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Brass
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Buchanan
authors.forename: Denice D
authors.initials: DD
authors.lastname: Tsao-Wei
authors.forename: Susan
authors.initials: S
authors.lastname: Groshen
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000378:metabolism*; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D016022:Case-Control Studies; D003094:Collagen / Q000494:pharmacology*; D003327:Coronary Disease / Q000097:blood*; D003920:Diabetes Mellitus / Q000097:blood*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D015539:Platelet Activation; D010974:Platelet Aggregation; D011446:Prospective Studies; D020239:Receptor Cross-Talk; D017452:Receptors, IgG / Q000378:metabolism*; D013268:Stimulation, Chemical
chemical_list: D015703:Antigens, CD; C080568:Fc gamma receptor IIA; D017452:Receptors, IgG; D003094:Collagen