Pyruvate preserves neutrophilic superoxide production in acidic, high glucose-enriched peritoneal dialysis solutions.
OBJECTIVE
To investigate effects of pyruvate (Py)-based peritoneal dialysis solutions (P-PDS) on neutrophilic superoxide (O2-) production against high glucose (HG) concentrations at acidic or physiologic pH value, and explore potential mechanisms.


METHODS
Human neutrophils were incubated with both dl-lactate (La, 40 mM)-based PDS (L-PDS) and equimolar P-PDS at various pH and HG levels, respectively. Hanks' balanced salt solution (HBSS) served as controls. O2- generation was determined by the reduction of ferricytochrome c.


RESULTS
Acidic pH and high La induced acute and substantial inhibitions of O2- production. HG in both PDS and HBSS resulted in a suppression of O2- in a dose-dependent manner. P-PDS generated near twofold O2- formation of L-PDS counterparts at various pH and HG levels. P-PDS with HG produced significantly more O2- than Py-free HBSS counterparts.


CONCLUSIONS
Py in PDS effectively protected neutrophils from HG-induced inhibition of O2- production, even at a physiological pH. The Py cytoprotection may be associated with the preservation of carbohydrate metabolic pathways in addition to its alkalization.
Wu
Yi Tai
YT
Department of Nephrology, Tong Ji Hospital, Tong Ji University School of Medicine, Shanghai, China.
Wu
Zhao Long
ZL
Jiang
Xiao Feng
XF
Li
Su
S
Zhou
Fang Qiang
FQ
D000138:Acidosis / Q000209:etiology* / Q000517:prevention & control; D002470:Cell Survival / Q000187:drug effects; D015314:Dialysis Solutions / Q000009:adverse effects* / Q000494:pharmacology*; D005947:Glucose / Q000009:adverse effects* / Q000494:pharmacology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism*; D016877:Oxidants / Q000032:analysis*; D010530:Peritoneal Dialysis / Q000009:adverse effects*; D019289:Pyruvic Acid / Q000494:pharmacology*; D013481:Superoxides / Q000032:analysis*
D015314:Dialysis Solutions; D016877:Oxidants; D013481:Superoxides; D019289:Pyruvic Acid; D005947:Glucose