Liposome entrapment and immunogenic studies of a synthetic lipophilic multiple antigenic peptide bearing VP1 and VP3 domains of the hepatitis A virus: a robust method for vaccine design.
Multiple antigen peptides (MAP) have been demonstrated to be efficient immunological reagents for the induction of immune responses to a variety of infectious agents. Several peptide domains of the hepatitis A virus (HAV) capsid proteins, mainly VP1 and VP3, are the immunodominant targets for a protective antibody response. In the present study we analyse the immunogenic properties of a tetrameric heterogeneous palmitoyl-derivatised MAP containing two defined HAV peptide sequences, VP1(11-25) and VP3(102-121), in rabbits immunised with either Freund's adjuvant or multilamellar liposomes. The immune response was evaluated with a specific enzyme immunoassay using MAP[VP1+VP3], VP1 and VP3 as targets. The avidity of the immune response was measured by a non-competitive enzyme-linked immunosorbent assay and by the surface plasmon resonance technology. Antisera raised against the lipo-MAP peptide entrapped in liposomes demonstrated high avidity of binding with affinity rate constants approximately one order of magnitude greater than those obtained with the Freund's protocol.
Haro
Isabel
I
Departament de Química de Pèptids i Proteïnes, IIQAB-CSIC, Jordi Girona 18-26, 08034 Barcelona, Spain. ihvqpp@iiqab.csic.es
Pérez
Silvia
S
García
Mónica
M
Chan
Weng C
WC
Ercilla
Guadalupe
G
D000595:Amino Acid Sequence; D006506:Hepatitis A / Q000276:immunology*; D022362:Hepatitis A Vaccines / Q000276:immunology*; D007700:Kinetics; D008081:Liposomes; D020349:Surface Plasmon Resonance; D014764:Viral Proteins / Q000276:immunology*; D015678:Viral Structural Proteins / Q000276:immunology*
D022362:Hepatitis A Vaccines; D008081:Liposomes; C096539:VP1 protein, hepatitis A virus; D014764:Viral Proteins; D015678:Viral Structural Proteins