title: Histopathological alterations of white seabass, Lates calcarifer, in acute and subchronic cadmium exposure.
abstract: Histopathological alterations to white seabass, Lates calcarifer aged 3 months in acute and subchronic cadmium exposure were studied by light and scanning electron microscopy. The 96-h LC50 values of cadmium to L. calcarifer was found to be 20.12 +/- 0.61 mg/l and the maximum acceptable toxicant concentration (MATC) was 7.79 mg/l. Fish were exposed to 10 and 0.8 mg/l of Cd (as CdCl,H2O) for 96 h and 90 days, respectively. The study showed that gill lamellae and kidney tubules were the primary target organs for the acute toxic effect of cadmium while in the subchronic exposure, the toxic effect to gills was less than that of kidney and liver. Gill alterations included edema of the epithelial cells with the breakdown of pillar cell system, aneurisms with some ruptures, hypertrophy and hyperplasia of epithelial and chloride cells. The liver showed blood congestion in sinusoids and hydropic swelling of hepatocytes, vacuolation and dark granule accumulation. Lipid droplets and glycogen content were observed in hepatocytes at the second and third month of subchronic exposure. The kidney showed hydropic swelling of tubular cell vacuolation and numerous dark granule accumulation in many tubules. Tubular degeneration and necrosis were seen in some areas.
authors.affiliation: Department of Biology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
authors.forename: S
authors.initials: S
authors.lastname: Thophon
authors.forename: M
authors.initials: M
authors.lastname: Kruatrachue
authors.forename: E S
authors.initials: ES
authors.lastname: Upatham
authors.forename: P
authors.initials: P
authors.lastname: Pokethitiyook
authors.forename: S
authors.initials: S
authors.lastname: Sahaphong
authors.forename: S
authors.initials: S
authors.lastname: Jaritkhuan
mesh_terms: D000818:Animals; D001492:Bass / Q000378:metabolism; D002104:Cadmium / Q000493:pharmacokinetics / Q000633:toxicity*; D002105:Cadmium Poisoning / Q000378:metabolism / Q000473:pathology*; D005880:Gills / Q000187:drug effects / Q000648:ultrastructure; D006651:Histocytochemistry; D007684:Kidney Tubules / Q000187:drug effects / Q000648:ultrastructure; D007928:Lethal Dose 50; D008099:Liver / Q000187:drug effects / Q000648:ultrastructure; D020714:Maximum Tolerated Dose; D008855:Microscopy, Electron, Scanning
chemical_list: D002104:Cadmium