Characterization and sequence of tomato 2S seed albumin: a storage protein with sequence similarities to the fruit lectin.
We found a 2S storage albumin from the seed of tomato ( Lycopersicon esculentum L. cv. Cherry) that cross-reacted with antiserum to the fruit lectin, and named it Lec2SA. According to its size and basicity, Lec2SA was classified into four isoforms. These isoforms have an M(r) of approximately 12,000, and are composed of a small subunit (M(r) 4,000) and a large subunit (M(r) 8,000) linked by disulfide bonds. The complete amino acid sequence of Lec2SA was determined. The small subunit was composed of 32 amino acids, whereas the large subunit contained 70 amino acids with a pyroglutamine as the N-terminal residue. The sequence of Lec2SA was similar to that of 2S albumins from different plants, such as Brazil nut and castor beans. Furthermore, a sequence similarity was found between the large subunit of Lec2SA and the peptide sequence from tomato lectin. Although these similarities were found, Lec2SA did not show hemagglutinating activity or sugar-chain-binding activity, indicating that Lec2SA lacks the carbohydrate-binding domain. These results suggest that tomato lectin is a chimeric lectin sharing the seed storage protein-like domain that is incorporated into the gene encoding tomato lectin through gene fusion.
Oguri
Suguru
S
Department of Bioproduction, Faculty of Bioindustry, Tokyo University of Agriculture, 099-2493, Abashiri, Hokkaido, Japan. s-oguri@bioindustry.nodai.ac.jp
Kamoshida
Mayumi
M
Nagata
Yoshiho
Y
Momonoki
Yoshie S
YS
Kamimura
Hideo
H
D000418:Albumins / Q000737:chemistry* / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D005638:Fruit / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry*; D018551:Solanum lycopersicum / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D020033:Protein Isoforms / Q000737:chemistry; D021122:Protein Subunits / Q000737:chemistry; D012639:Seeds / Q000737:chemistry*; D016415:Sequence Alignment; D013045:Species Specificity
D000418:Albumins; D037102:Lectins; D010940:Plant Proteins; D020033:Protein Isoforms; D021122:Protein Subunits