Molecular detection of Histoplasma capsulatum var. capsulatum in human clinical samples.
We developed a seminested PCR for the diagnosis of histoplasmosis that amplifies a portion of the Histoplasma capsulatum H antigen gene. This assay is highly sensitive and specific, being able to detect genomic material corresponding to less than 10 yeast cells without cross-reaction against other bacterial or fungal pathogens.
Bracca
Andrea
A
Instituto de Biología Molecular y Celular de Rosario, IBR-CONICET, Facultad de Ciencias Bioquímicas y Farmacéuticas, 2000 Rosario, Argentina.
Tosello
María Elena
ME
Girardini
Javier E
JE
Amigot
Susana L
SL
Gomez
Carlos
C
Serra
Esteban
E
D000946:Antigens, Fungal / Q000235:genetics; D003470:Culture Media; D004271:DNA, Fungal / Q000032:analysis; D006658:Histoplasma / Q000235:genetics / Q000302:isolation & purification*; D006660:Histoplasmosis / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods
D000946:Antigens, Fungal; D003470:Culture Media; D004271:DNA, Fungal