Spinal adenosine agonist reduces c-fos and astrocyte activation in dorsal horn of rats with adjuvant-induced arthritis.
Adjuvant-induced arthritis (AIA) is a useful animal model of rheumatoid arthritis (RA). We have previously shown that stimulation of spinal adenosine A1 receptors decreases peripheral signs of AIA. In this study we show that when the final dose of adenosine A1 agonist is given 1 h prior to sacrifice the number of c-Fos positive cells evoked by the AIA in both the superficial and deep dorsal horns decreases by about 50%. In saline-treated AIA animals the increase in c-Fos expression seen in laminae I-IV was accompanied by an increase in glial fibrillary acidic protein immunoreactivity, indicative of astrocyte activation. Astrocyte activation was only partially attenuated by adenosine A1 receptor agonist treatment.
Sorkin
Linda S
LS
Department of Anesthesiology and Division of Rheumatology, UCSD School of Medicine, 9500 Gilman Drive, La Jolla, CA 92093-0818, USA. lsorkin@ucsd.edu
Maruyama
Karly
K
Boyle
David L
DL
Yang
Li
L
Marsala
Martin
M
Firestein
Gary S
GS
D000241:Adenosine / Q000008:administration & dosage* / Q000031:analogs & derivatives*; D000818:Animals; D001169:Arthritis, Experimental / Q000188:drug therapy / Q000378:metabolism*; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D016762:Genes, fos / Q000187:drug effects* / Q000502:physiology; D007278:Injections, Spinal; D008297:Male; D020671:Posterior Horn Cells / Q000187:drug effects* / Q000378:metabolism; D058906:Purinergic P1 Receptor Agonists; D051381:Rats; D011917:Rats, Inbred Lew; D018047:Receptors, Purinergic P1 / Q000378:metabolism
D058906:Purinergic P1 Receptor Agonists; D018047:Receptors, Purinergic P1; C027513:N(6)-cyclohexyladenosine; D000241:Adenosine