Cloning and characterization of the Atlantic salmon serum lectin, a long-form C-type lectin expressed in kidney.
We report the cloning of four distinct cDNAs and a genomic sequence encoding a multimeric serum lectin found in the blood of Atlantic salmon (Salmo salar). The sequence variation among the cDNAs as well as genomic Southern blotting analysis revealed a multi-gene family. Expression of the salmon serum lectin (SSL) was specific to kidney, as demonstrated by RT-PCR. Analysis of the 173-amino acid sequence of SSL confirmed that it is a member of the C-type lectin superfamily. Sequence alignments and intron/exon structure of the SSL gene showed it to belong to the type VII C-type lectins, which normally bind to galactose or other ligands, whereas the SSL protein sequence contains the EPN motif of mannose-binding C-type lectins, that bind mannose or related carbohydrates.
Richards
R C
RC
NRC Institute for Marine Biosciences, 1411 Oxford Street, B3H 3Z1, Halifax, Nova Scotia, Canada.
Hudson
D M
DM
Thibault
P
P
Ewart
K V
KV
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis; D007668:Kidney / Q000378:metabolism; D037181:Lectins, C-Type / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020317:Salmo salar / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment
D018076:DNA, Complementary; D037181:Lectins, C-Type; D020033:Protein Isoforms; D012333:RNA, Messenger