Tendon healing in vitro: promotion of collagen gene expression by bFGF with NF-kappaB gene activation.
OBJECTIVE
Matrix synthesis of intrasynovial tendon cells and activation of nuclear transcription factors are pivotal to promotion of flexor tendon healing. We investigated the effects of basic fibroblast growth factor (bFGF) on synthesis of type I collagen and activation of nuclear transcription factor kappaB (NF-kappaB) in an in vitro culture model of intrasynovial tenocytes.


METHODS
Tenocytes obtained from explant culture of rabbit intrasynovial tendon segments were treated with bFGF at concentrations of 0, 2, and 10 ng/mL. Expression of type I collagen and NF-kappaB genes was determined by quantitative analysis of products of reverse-transcription polymerase chain reactions. Proliferation of the cells was assessed by incorporation of bromodeoxyuridine into the DNA of the cells.


RESULTS
Expression levels of type I collagen and NF-kappaB genes of tenocytes were increased significantly by bFGF. Cell proliferation as indicated by DNA labeling was promoted significantly by bFGF. Expression of the NF-kappaB gene increased proportionately to the amounts of bFGF stimulating the cells and was correlated with increases in proliferation rate of tenocytes.


CONCLUSIONS
Results of this study show that expression of type I collagen and NF-kappaB genes is promoted manifestly by bFGF. The effects were proportionate to in vitro proliferation rates of tenocytes. The study indicated that matrix synthesis of flexor tendons can be promoted by bFGF and that NF-kappaB may play a pivotal role in initiating proliferation and type I collagen synthesis of tenocytes.
Tang
Jin Bo
JB
Hand Surgery Research Center, Department of Hand Surgery, Affiliated Hospital of Nantong Medical College, Nantong, Jiangsu, China.
Xu
Yan
Y
Ding
Fei
F
Wang
Xiao Tian
XT
D000704:Analysis of Variance; D000818:Animals; D018929:Cell Culture Techniques; D024042:Collagen Type I / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005786:Gene Expression Regulation; D016328:NF-kappa B / Q000235:genetics*; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013708:Tendon Injuries / Q000378:metabolism*; D015533:Transcriptional Activation; D014945:Wound Healing / Q000502:physiology*
D024042:Collagen Type I; D016328:NF-kappa B; D016222:Fibroblast Growth Factor 2