Chromatin immunoprecipitation reveals that the 180-bp satellite repeat is the key functional DNA element of Arabidopsis thaliana centromeres.
The centromeres of Arabidopsis thaliana chromosomes contain megabases of complex DNA consisting of numerous types of repetitive DNA elements. We developed a chromatin immunoprecipitation (ChIP) technique using an antibody against the centromeric H3 histone, HTR12, in Arabidopsis. ChIP assays showed that the 180-bp centromeric satellite repeat was precipitated with the antibody, suggesting that this repeat is the key component of the centromere/kinetochore complex in Arabidopsis.
Nagaki
Kiyotaka
K
Department of Horticulture, University of Wisconsin, Madison, Wisconsin 53706, USA.
Talbert
Paul B
PB
Zhong
Cathy Xiaoyan
CX
Dawe
R Kelly
RK
Henikoff
Steven
S
Jiang
Jiming
J
D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics; D002503:Centromere / Q000235:genetics*; D002843:Chromatin / Q000302:isolation & purification*; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics*
D029681:Arabidopsis Proteins; D002843:Chromatin; D018744:DNA, Plant