The C-terminal region of cellular Qin oligomerizes: correlation with oncogenic transformation and transcriptional repression.
Expression of the oncoprotein Qin induces tumors in chickens and oncogenic transformation of chicken embryo fibroblasts in culture. We performed a detailed deletion analysis of the C-terminal region of Qin (amino acids 246-451, extending from the winged helix domain to the C-terminus) and identified amino acids 246-379 as important for transformation. The same region mediates homo-oligomerization of Qin as documented in vitro by GST pulldowns and in vivo by coimmunoprecipitation. A 60 amino-acid region within the oligomerization domain is necessary and sufficient for transcriptional repression induced by Qin.
Sonderegger
Corinna K
CK
Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA.
Narisawa-Saito
Mako
M
Vogt
Peter K
PK
D000818:Animals; D030161:Avian Proteins; D001704:Biopolymers; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000502:physiology; D005347:Fibroblasts; D051858:Forkhead Transcription Factors; D006801:Humans; D007668:Kidney; D015513:Oncogene Proteins; D010446:Peptide Fragments / Q000737:chemistry; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011518:Proto-Oncogene Proteins / Q000737:chemistry* / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000502:physiology; D012097:Repressor Proteins / Q000737:chemistry* / Q000502:physiology; D017384:Sequence Deletion; D013329:Structure-Activity Relationship; D014158:Transcription, Genetic; D014162:Transfection; D014764:Viral Proteins
D030161:Avian Proteins; D001704:Biopolymers; D004268:DNA-Binding Proteins; D051858:Forkhead Transcription Factors; D015513:Oncogene Proteins; D010446:Peptide Fragments; D011518:Proto-Oncogene Proteins; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D014764:Viral Proteins; C490425:v-qin protein, Avian sarcoma virus 31