Effects of dietary fatty acids on burn-induced immunosuppression.
Previous studies from our laboratory established that low-fat diets prevent immunosuppression and reduce oxidative stress after a thermal injury. The purpose of the present study was to test the hypothesis that the type of dietary fatty acid influences splenocyte proliferation and oxidative stress following a burn injury. Female C3H/HeN mice were fed ad libitum six experimental diets (5% w/w lipids) differing in fatty acid composition for 10 days following a burn injury. Compared to the controls, burned mice fed whichever diet showed lower lymphoproliferative responses to concanavalin-A (Con-A) and lipopolysaccharide (LPS) (p<0.01), but not to an anti-T cell receptor monoclonal antibody (H-57). In burned animals, nitric oxide (NO) concentration was negatively correlated to the proliferation induced by Con-A (p<0.01) or LPS (p<0.05). These results suggest that: (1) dietary fatty acid type does not influence the splenocyte proliferation or oxidative stress and (2) NO production is involved in the immunosuppression following burn injury.
Bordé
Viviane D
VD
Centre des grands brûlés de Montréal, Centre de recherche hospitalier de l'Université de Montréal-Pavillon Hôtel-Dieu, Montréal, Qué., Canada.
Bernier
Jacques
J
Garrel
Dominique R
DR
D000818:Animals; D002056:Burns / Q000276:immunology* / Q000378:metabolism; D003208:Concanavalin A / Q000276:immunology; D004041:Dietary Fats / Q000276:immunology* / Q000494:pharmacology; D005227:Fatty Acids / Q000276:immunology* / Q000494:pharmacology; D005260:Female; D008070:Lipopolysaccharides / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008809:Mice, Inbred C3H; D009569:Nitric Oxide / Q000276:immunology / Q000378:metabolism; D018384:Oxidative Stress / Q000276:immunology; D011897:Random Allocation; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology / Q000378:metabolism
D004041:Dietary Fats; D005227:Fatty Acids; D008070:Lipopolysaccharides; D003208:Concanavalin A; D009569:Nitric Oxide