Macroaffinity ligand-facilitated three-phase partitioning (MLFTPP) of alpha-amylases using a modified alginate.
The crude extracts of alpha-amylases when mixed with alginate, tert-butyl alcohol, and ammonium sulfate resulted in an interfacial precipitate containing polymer-bound amylase. The precipitate was dissolved in 1 M maltose to recover alpha-amylase activity. The recovery of alpha-amylases were 74%, 77%, and 92% in the case of Bacillus amyloliquefaciens, wheat germ, and porcine pancreas, respectively. All purified preparations showed a single band on SDS-PAGE.
Mondal
Kalyani
K
Chemistry Department, Indian Institute of Technology, Delhi Hauz Khas, New Delhi 110016, India.
Sharma
Aparna
A
Lata
Lata
L
Gupta
Munishwar Nath
MN
D000327:Adsorption; D000464:Alginates / Q000737:chemistry*; D001407:Bacillus / Q000737:chemistry / Q000201:enzymology; D002846:Chromatography, Affinity / Q000379:methods*; D008024:Ligands; D010179:Pancreas / Q000737:chemistry / Q000201:enzymology; D014908:Triticum / Q000737:chemistry / Q000201:enzymology; D000516:alpha-Amylases / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism
D000464:Alginates; D008024:Ligands; D000516:alpha-Amylases