Molecular breeding of transgenic rice expressing a xylanase domain of the xynA gene from Clostridium thermocellum.
The gene encoding the catalytic domain of thermostable xylanase from Clostridium thermocellum F1 was expressed in rice plants under the control of a constitutive promoter. The gene encoding Xylanase A was modified to encode the catalytic domain of family 11 xylanase without the signal sequence (xynA1), and was introduced into rice plants and expressed under the control of a modified cauliflower mosaic virus 35S promoter. Zymogram analysis indicated that the recombinant xylanase was produced in rice plants. The xynA1 gene was stably expressed in rice straw and seed grains. No phenotypic effect of xylanase expression was noted. The enzyme was detected in the desiccated grain. High levels of enzyme activity were maintained in the cell-free extract during incubation at 60 degrees C for 24 h. The results indicated that high levels of xylanase can be produced in rice plants.
Kimura
T
T
Faculty of Bioresources, Mie University, Kamihama-cho, Tsu, 514-8507 Mie, Japan. t-kimura@bio.mie-u.ac.jp
Mizutani
T
T
Tanaka
T
T
Koyama
T
T
Sakka
K
K
Ohmiya
K
K
D001426:Bacterial Proteins / Q000235:genetics; D001709:Biotechnology / Q000379:methods; D003013:Clostridium / Q000201:enzymology* / Q000235:genetics; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004795:Enzyme Stability; D012275:Oryza / Q000201:enzymology* / Q000235:genetics*; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D014170:Transformation, Genetic; D014990:Xylans / Q000378:metabolism
D001426:Bacterial Proteins; D014990:Xylans; D043364:Endo-1,4-beta Xylanases