title: Dna2 requirement for normal reproduction of Caenorhabditis elegans is temperature-dependent.
abstract: To determine the function of Dna2 in a multicellular organism, the Caenorhabditis elegans Dna2 expression was probed and deletion mutant phenotypes were analyzed. Dna2 was localized to the nuclei of C. elegans oocytes and early embryos by immunostaining or green fluorescent protein-tagging. A homozygous dna2 deletion mutant showed a reduced brood size and embryonic lethality, and the phenotypes greatly depended on growth temperature and aggravated in the succeeding generation. The mutant embryos also showed delayed cell divisions, which together with temperature-dependence of the mutant phenotypes supported the well-conserved role of Dna2 in DNA replication.
authors.affiliation: Department of Biochemistry, College of Science, Yonsei University, Seoul 120-749, Korea.
authors.forename: Kyoung-Hwa
authors.initials: KH
authors.lastname: Lee
authors.forename: Myon Hee
authors.initials: MH
authors.lastname: Lee
authors.forename: Tae Hoon
authors.initials: TH
authors.lastname: Lee
authors.forename: Ji Won
authors.initials: JW
authors.lastname: Han
authors.forename: Yong Jin
authors.initials: YJ
authors.lastname: Park
authors.forename: Joohong
authors.initials: J
authors.lastname: Ahnn
authors.forename: Yeon-Soo
authors.initials: YS
authors.lastname: Seo
authors.forename: Hyeon-Sook
authors.initials: HS
authors.lastname: Koo
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics / Q000502:physiology*; D000595:Amino Acid Sequence; D000818:Animals; D020132:Anticipation, Genetic; D017173:Caenorhabditis elegans / Q000196:embryology / Q000235:genetics / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000502:physiology*; D002455:Cell Division; D004265:DNA Helicases / Q000235:genetics / Q000502:physiology*; D004261:DNA Replication; D018509:DNA, Helminth / Q000096:biosynthesis / Q000235:genetics; D005298:Fertility / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D005804:Genes, Lethal; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000032:analysis / Q000235:genetics; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism; D034622:RNA Interference; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013696:Temperature
chemical_list: D029742:Caenorhabditis elegans Proteins; D018509:DNA, Helminth; D008164:Luminescent Proteins; D029701:Saccharomyces cerevisiae Proteins; D049452:Green Fluorescent Proteins; D000251:Adenosine Triphosphatases; D004265:DNA Helicases; C410222:DNA2 protein, S cerevisiae