title: [Detection of SEN virus in sera of patients with chronic hepatitis B and general population in 5 cities of China].
abstract: OBJECTIVE
  To study the prevalence of SEN virus (SENV) infection in CHB patients in five cities of China.
  
  
  METHODS
  A nest-polymerase chain reaction (nPCR) was used for detection of SENV-D and SENV-H in sera of 595 CHB patients from 5 cities of China and 96 normal individuals from Beijing. A total of 7 SENV strains were analyzed by direct sequencing.
  
  
  RESULTS
  The prevalence rates of SENV in CHB patients and normal individuals were 61.3% and 62.5%, respectively (chi(2) = 0.047, P = 0.829). The prevalence rates of CHB patients between 5 cities were different. Nucleotide sequence analysis showed that the homology between 4 SENV-D strains was 91% - 98% and 95% - 98% between 3 SENV-H strains isolated from 5 cities in China.
  
  
  CONCLUSIONS
  SENV-D/H were prevalent in CHB patients of China and their prevalence rates were similar to that in normal individuals.
authors.affiliation: Beijing Ditan Hospital, Beijing 100011, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yan
authors.forename: Zhong-ping
authors.initials: ZP
authors.lastname: He
authors.forename: Hui
authors.initials: H
authors.lastname: Zhuang
authors.forename: Qing-ming
authors.initials: QM
authors.lastname: Dong
authors.forename: Shu-jing
authors.initials: SJ
authors.lastname: Song
authors.forename: Lin
authors.initials: L
authors.lastname: Zhu
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Wang
mesh_terms: D002681:China / Q000453:epidemiology; D018136:Circoviridae / Q000302:isolation & purification; D018173:Circoviridae Infections / Q000150:complications / Q000453:epidemiology / Q000821:virology; D004266:DNA Virus Infections / Q000150:complications / Q000453:epidemiology* / Q000821:virology; D004267:DNA Viruses / Q000302:isolation & purification*; D004279:DNA, Viral / Q000032:analysis; D019694:Hepatitis B, Chronic / Q000150:complications / Q000821:virology*; D006801:Humans; D010802:Phylogeny; D015995:Prevalence
chemical_list: D004279:DNA, Viral