title: Isolation and characterization of an atrazine-degrading bacterium from industrial wastewater in China.
abstract: OBJECTIVE
  To isolate and characterize atrazine-degrading bacteria in order to identify suitable candidates for potential use in bioremediation of atrazine contamination.
  
  
  RESULTS
  A high efficiency atrazine-degrading bacterium, strain AD1, which was capable of utilizing atrazine as a sole nitrogen source for growth, was isolated from industrial wastewater. 16S rDNA sequencing identified AD1 as an Arthrobacter sp. The atrazine chlorohydrolase gene (atzA) isolated from strain AD1 differed from that found in the Pseudomonas sp. ADP by only one nucleotide. However, it was found located on the bacterial chromosome rather than on plasmids as previously reported for other bacteria.
  
  
  CONCLUSIONS
  Atrazine chlorohydrolase gene, atzA, either encoded by chromosome or plasmid, is highly conserved.
  
  
  CONCLUSIONS
  Comparison analysis of atrazine degradation gene structure and arrangement in this and other bacteria provides insight into our understanding of the ecology and evolution of atrazine-degrading bacteria.
authors.affiliation: College of Life Sciences, Naikai University, Tianjin, China.
authors.forename: B
authors.initials: B
authors.lastname: Cai
authors.forename: Y
authors.initials: Y
authors.lastname: Han
authors.forename: B
authors.initials: B
authors.lastname: Liu
authors.forename: Y
authors.initials: Y
authors.lastname: Ren
authors.forename: S
authors.initials: S
authors.lastname: Jiang
mesh_terms: D001173:Arthrobacter / Q000145:classification / Q000201:enzymology / Q000302:isolation & purification* / Q000378:metabolism*; D001280:Atrazine / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002681:China; D004785:Environmental Pollutants / Q000378:metabolism*; D006867:Hydrolases / Q000378:metabolism; D007220:Industrial Waste; D014871:Water Microbiology
chemical_list: D004785:Environmental Pollutants; D007220:Industrial Waste; D006867:Hydrolases; C100894:atrazine chlorohydrolase; D001280:Atrazine