The influence of physico-chemical and process conditions on the physical stability of plasmid DNA complexes using response surface methodology.
Research is progressing fast to find safe and effective methods of delivering therapeutic genes to patients afflicted with a range of genetic and acquired diseases that either do not respond at all, or respond poorly, to treatment with small-molecule drugs or protein-replacement therapy. A technical barrier that remains relates to the need for scalable operations that can consistently and reproducibly make large quantities of the therapeutic gene vectors under the current Good Manufacturing Practice ('cGMP'). The present investigation focuses on these issues and introduces a new method of assessing the engineering effects of process and material factors on the colloidal properties of plasmid-DNA delivery systems based on response surface methodology (RSM) and experimental techniques. Previously, experiments have shown that several factors can reduce the physical stability of non-viral delivery systems. Specifically, it has been demonstrated that the mean size and charge of plasmid DNA condensed by cationic agents are affected by many factors, including the pH and ionic strength of the buffer, and the method of preparation. For example, the method and intensity of mixing of the DNA with condensing and conjugating agents have been shown to be important. Using RSM to analyse new experimental data in the present paper, we report on the impact of these factors and, more crucially, the effects of interaction between the factors on the colloidal properties of the DNA-vector complexes. Specifically, for plasmid DNA condensed by poly-L-lysine, interactions between ionic strength, pH and DNA concentrations play a critical role. Whether poly-L-lysine should be used as a condensing agent in the final delivery system remains to be demonstrated. However, the use of RSM combined with the scaleable experimental approach described in this paper may be applied to other delivery systems.
Mount
Clare N
CN
Department of Biochemical Engineering, University College London, Torrington Place, London WC1E 7JE, UK.
Lee
Li K
LK
Yasin
Ahmad
A
Scott
Andrew
A
Fearn
Tom
T
Shamlou
Parviz Ayazi
PA
D003102:Colloids / Q000737:chemistry*; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D016503:Drug Delivery Systems / Q000379:methods*; D004355:Drug Stability; D006863:Hydrogen-Ion Concentration; D008081:Liposomes / Q000737:chemistry*; D008422:Materials Testing / Q000379:methods*; D010316:Particle Size; D010957:Plasmids / Q000008:administration & dosage / Q000737:chemistry*; D011107:Polylysine / Q000737:chemistry*; D012492:Salts / Q000737:chemistry; D013223:Statistics as Topic
D003102:Colloids; D008081:Liposomes; D012492:Salts; D011107:Polylysine