title: Genetic instability in superficial bladder cancer and adjacent mucosa: an interphase cytogenetic study.
abstract: A systematic analysis of both tumors and the surrounding urothelium to help identify what lies behind the mechanism of multifocal tumor development has not yet been performed. In this study we investigated chromosome 1, 7, 9, and 17 aneusomy in 25 superficial papillary carcinomas and in 51 tissue samples taken from sites of macroscopically uninvolved urothelium surrounding the tumors, using the fluorescence in situ hybridization method. Our data demonstrated a close genetic relationship between all examined tumors and normal-appearing mucosa. Numeric aberrations of chromosomes 1, 7, 9, and 17 were found to exhibit similar patterns in all analyzed specimens, although with different frequencies.
authors.affiliation: Department of Clinical Pathology, Regina Elena Cancer Institute, Rome, Italy.
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Cianciulli
authors.forename: Costantino
authors.initials: C
authors.lastname: Leonardo
authors.forename: Fiorella
authors.initials: F
authors.lastname: Guadagni
authors.forename: Raffaella
authors.initials: R
authors.lastname: Marzano
authors.forename: Francesco
authors.initials: F
authors.lastname: Iori
authors.forename: Cosimo
authors.initials: C
authors.lastname: De Nunzio
authors.forename: Giorgio
authors.initials: G
authors.lastname: Franco
authors.forename: Roberta
authors.initials: R
authors.lastname: Merola
authors.forename: Cesare
authors.initials: C
authors.lastname: Laurenti
mesh_terms: D002291:Carcinoma, Papillary / Q000235:genetics*; D002869:Chromosome Aberrations; D002878:Chromosomes, Human, Pair 1; D002886:Chromosomes, Human, Pair 17; D002897:Chromosomes, Human, Pair 7; D002899:Chromosomes, Human, Pair 9; D020732:Cytogenetic Analysis; D005456:Fluorescent Dyes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007211:Indoles; D009092:Mucous Membrane / Q000648:ultrastructure; D001749:Urinary Bladder Neoplasms / Q000235:genetics*; D019459:Urothelium / Q000648:ultrastructure
chemical_list: D005456:Fluorescent Dyes; D007211:Indoles; C007293:DAPI