ASAP, a novel protein complex involved in RNA processing and apoptosis.
Different isoforms of a protein complex termed the apoptosis- and splicing-associated protein (ASAP) were isolated from HeLa cell extract. ASAP complexes are composed of the polypeptides SAP18 and RNPS1 and different isoforms of the Acinus protein. While Acinus had previously been implicated in apoptosis and was recently identified as a component of the spliceosome, RNPS1 has been described as a general activator of RNA processing. Addition of ASAP isoforms to in vitro splicing reactions inhibits RNA processing mediated by ASF/SF2, by SC35, or by RNPS1. Additionally, microinjection of ASAP complexes into mammalian cells resulted in acceleration of cell death. Importantly, after induction of apoptosis the ASAP complex disassembles. Taken together, our results suggest an important role for the ASAP complexes in linking RNA processing and apoptosis.
Schwerk
Christian
C
Howard Hughes Medical Institute and Division of Nucleic Acids Enzymology, Department of Biochemistry, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA.
Prasad
Jayendra
J
Degenhardt
Kurt
K
Erdjument-Bromage
Hediye
H
White
Eileen
E
Tempst
Paul
P
Kidd
Vincent J
VJ
Manley
James L
JL
Lahti
Jill M
JM
Reinberg
Danny
D
D017209:Apoptosis / Q000502:physiology*; D002352:Carrier Proteins; D056970:Co-Repressor Proteins; D006367:HeLa Cells; D006801:Humans; D066298:In Vitro Techniques; D046911:Macromolecular Substances; D009687:Nuclear Proteins / Q000737:chemistry* / Q000378:metabolism*; D020033:Protein Isoforms / Q000737:chemistry / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012326:RNA Splicing; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins / Q000737:chemistry / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism
C121310:ACIN1 protein, human; D002352:Carrier Proteins; D056970:Co-Repressor Proteins; D046911:Macromolecular Substances; D009687:Nuclear Proteins; D020033:Protein Isoforms; D016601:RNA-Binding Proteins; C084538:RNPS1 protein, human; D012261:Ribonucleoproteins; C498650:SAP18 protein, human; D014157:Transcription Factors