Differential inhibitory action of two azoic compounds against arenaviruses.
The action of five azo-based compounds against the arenaviruses Junin (JUNV) and Tacaribe (TCRV) was evaluated in vitro by a virus yield inhibition assay in Vero cells and a cell-free virion inactivation assay. The compound 2-azo-(1'-(2'-nitroso)naphthyl)-benzoate (ANNB) was the most effective inhibitor of arenavirus production in Vero cells with EC(50) (effective concentration 50%) values in the range 6.5-26.2 microM and without inactivating properties. By contrast, the azodicarbonamide (ADA) was very effective in inactivating both arenaviruses with IC(50) (inactivating concentration 50%) values of 7.6 and 5.3 microM against JUNV and TCRV, respectively. The virucidal activity of ADA was time- and temperature-dependent. ANNB had no inhibitory action on virus binding or penetration of target cells and did not affect the synthesis of viral proteins. The most likely event susceptible to ANNB would be the process of intracellular virion assembly.
García
Cybele C
CC
Facultad de Ciencias Exactas y Naturales, Laboratorio de Virología, Departamento de Química Biológica, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, Piso 4, Buenos Aires 1428, Argentina.
Candurra
Nélida A
NA
Damonte
Elsa B
EB
D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D006477:Arenaviruses, New World / Q000187:drug effects*; D001391:Azo Compounds / Q000494:pharmacology*; D002522:Chlorocebus aethiops; D066298:In Vitro Techniques; D018051:Junin virus / Q000187:drug effects* / Q000254:growth & development; D015394:Molecular Structure; D014709:Vero Cells; D014764:Viral Proteins
D000998:Antiviral Agents; D001391:Azo Compounds; D014764:Viral Proteins; C009850:azobenzene