Derepression of galactose metabolism in melibiase producing bakers' and distillers' yeast.
Beet molasses is widely used as a growth substrate for bakers' and distillers' yeast in the production of biomass and ethanol. Most commercial yeasts do not fully utilise the carbohydrates in molasses since they are incapable of hydrolysing the disaccharide melibiose to glucose and galactose. Also, expression of genes encoding enzymes for the utilisation of carbon sources that are alternatives to glucose is tightly regulated, sometimes rates of yeast growth and/or ethanol production. The GAL genes are regulated by specific induction by galactose and repression during growth on glucose. In an industrial distillers' yeast, two genes interacting synergistically in glucose repression of galactose utilization, MIG1 and GAL80, have been disrupted with MEL1, encoding melibiase. The physiology of the wild-type strain and the recombinant strains was investigated on mixtures of glucose and galactose and on molasses. The recombinant strain started to ferment galactose when 9.7 g 1(-1) glucose was still present during a batch fermentation, whereas the wild-type strain did not consume any galactose in the presence of glucose. The ethanol yield in the recombinant strain was 0.50 g ethanol g sugar (-1) in an ethanol fermentation on molasses, compared with 0.48 g ethanol g sugar (-1) for the wild-type strain. The increased ethanol yield was due to utilization of melibiose in the molasses.
Rønnow
B
B
Danisco Biotechnology, DK-1001, Copenhagen K, Denmark.
Olsson
L
L
Nielsen
J
J
Mikkelsen
J D
JD
D003470:Culture Media; D004794:Enzyme Repression; D004926:Escherichia coli / Q000235:genetics; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D005690:Galactose / Q000378:metabolism*; D008965:Molasses; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D014170:Transformation, Genetic; D000519:alpha-Galactosidase / Q000096:biosynthesis*
D003470:Culture Media; D000431:Ethanol; D000519:alpha-Galactosidase; D005690:Galactose