Molecular evidence for local denervation of paraspinal muscles in failed-back surgery/postdiscotomy syndrome.
A study was performed to analyze whether local denervation of the medial branch of the dorsal ramus of the lumbar spinal nerve occurs in a patient with postoperative failed-back surgery syndrome/postdiscotomy syndrome (FBSS/PDS). We investigated the effect of the loss of innervation of the multifidus muscle on neuronal nitrite oxide synthetase (n-NOS) and endothelial nitrite oxide synthetase (e-NOS) applying realtime RT-PCR and immunohistochemistry. Our study demonstrates a substantial reduction of n-NOS expression, supporting the view that local denervation of the multifidus is involved in the pathology of FBSS. No regulation of e-NOS was detectable. Interestingly, this change is region-specific and does not occur throughout the entire multifidus segment. This result supports the hypothesis that local denervation of the multifidus muscle is involved in the pathology of FBSS/ PDS.
Zoidl
G
G
Department of Neuroanatomy and Molecular Brain Research, Ruhr University, Bochum, Germany.
Grifka
J
J
Boluki
D
D
Willburger
R E
RE
Zoidl
C
C
Krämer
J
J
Dermietzel
R
R
Faustmann
P M
PM
D000368:Aged; D001706:Biopsy; D015536:Down-Regulation; D006801:Humans; D007405:Intervertebral Disc Displacement / Q000601:surgery; D007796:Laminectomy / Q000009:adverse effects*; D017116:Low Back Pain / Q000209:etiology*; D008159:Lumbar Vertebrae / Q000601:surgery; D008297:Male; D018482:Muscle, Skeletal / Q000294:innervation* / Q000378:metabolism / Q000473:pathology; D009134:Muscular Atrophy, Spinal / Q000209:etiology* / Q000473:pathology; D009474:Neurons / Q000201:enzymology; D019001:Nitric Oxide Synthase / Q000378:metabolism; D059348:Peripheral Nerve Injuries; D013577:Syndrome
D019001:Nitric Oxide Synthase