Flow-injection spectrophotometric determination of sulfadiazine and sulfamethoxazole in pharmaceuticals and urine.
A rapid and sensitive flow-injection spectrophotometric method is proposed for the determination of sulfadiazine and sulfamethoxazole. This method is based on the diazotization of sulfonamide with sodium nitrite, and a coupling reaction of the diazo-compound with alpha-naphthylamine. The optimum experimental conditions are obtained by using the controlled and weighted centroid simplex method. The linear ranges for the determination of sulfadiazine and sulfamethoxazole are 0.2-20 microg ml(-1) and 0.1-20 microg ml(-1), and their detection limits are 0.06 microg ml(-1) and 0.05 microg ml(-1), respectively, and the sampling frequency is 130 samples per hour. The method has been used to determine sulfadiazine and sulfamethoxazole in pharmaceuticals and urine without separation. The results are in agreement with those obtained by a high-performance liquid chromatograph technique at the 95% confidence level.
Fan
Jing
J
School of Chemical & Environmental Sciences, Henan Normal University, Key Laboratory of Environmental Science and Engineering of Henan Education Commission, Xinxiang, Henan 453002, People's Republic of China.
Chen
Yahong
Y
Feng
Suling
S
Ye
Cunling
C
Wang
Jianji
J
D017022:Flow Injection Analysis / Q000379:methods*; D006801:Humans; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D013411:Sulfadiazine / Q000032:analysis* / Q000652:urine*; D013420:Sulfamethoxazole / Q000032:analysis* / Q000652:urine*; D013607:Tablets / Q000737:chemistry; D013997:Time Factors
D004364:Pharmaceutical Preparations; D013607:Tablets; D013411:Sulfadiazine; D013420:Sulfamethoxazole