title: Amino acid sequence of the 26 kDa subunit of legumin-type seed storage protein of common buckwheat (Fagopyrum esculentum Moench): molecular characterization and phylogenetic analysis.
abstract: The paper describes the amino acid sequence of a 26 kDa basic subunit of 13S globulin of common buckwheat (Fagopyrum esculentum Moench). The protein has 93 and 75% sequence homology with 11S globulin of Coffea arabica and beta subunit of 11S globulin of Cucurbita pepo respectively. The subunit has the "globally conserved" N-terminal sequence consisting of Gly-Ile-Asp-Glu and the cysteine at P7' from the proteolytic processing site. A conserved 7 residue domain of Pro-His-Trp-Asn-Ile-Asn-Ala, characteristic of basic subunits of legumins from non-leguminous angiosperms, is also present in this protein. A distinguishing features of this subunit is the relatively high level of lysine and methionine.
authors.affiliation: Department of Botany, North Eastern Hill University Shillong 793 022, India.
authors.forename: Sangeeta
authors.initials: S
authors.lastname: Bharali
authors.forename: Nikhil K
authors.initials: NK
authors.lastname: Chrungoo
mesh_terms: D000595:Amino Acid Sequence; D017124:Conserved Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D019612:Fagopyrum / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D021122:Protein Subunits / Q000235:genetics; D012639:Seeds / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D000097793:Legumins
chemical_list: D010940:Plant Proteins; D021122:Protein Subunits; D000097793:Legumins