Effect of chlorhexidine on molecular weight distribution of fructans produced by fructosyltransferase in solution and immobilized on surface.
The effect of chlorhexidine (CHX), a potent antibacterial agent, was tested on the molecular weight distribution (MWD) of fructans synthesized by cell-free fructosyltransferase (FTF) in solution in comparison to FTF immobilized onto hydroxyapatite (HA). Size-exclusion chromatography (SEC) analysis has shown that cell-free FTF, both in solution and immobilized on HA, produces both low MW (1.9-2.2 kDa) and high MW (913-1047 kDa) fructans. CHX at a concentration of 0.02% altered the MWD of the fructans by reducing the polydispersity ratio and changing the MWD of the fructans synthesized both by immobilized FTF and by FTF in solution. These changes of the fructans in the presence of CHX adds a new prospective to the anticaries effect of CHX in addition to its antibacterial properties.
Rozen
Ramona
R
Institute of Dental Sciences, Faculty of Dentistry, Hebrew University-Hadassah, PO Box 12272 91120, Jerusalem, Israel.
Bachrach
Gilad
G
Zaks
Batia
B
Bronshteyn
Moshe
M
Gedalia
Itzhak
I
Steinberg
Doron
D
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002710:Chlorhexidine / Q000494:pharmacology*; D005630:Fructans / Q000737:chemistry* / Q000378:metabolism; D006602:Hexosyltransferases / Q000378:metabolism*; D006882:Hydroxyapatites / Q000737:chemistry; D008970:Molecular Weight; D001426:Bacterial Proteins
D000900:Anti-Bacterial Agents; D002710:Chlorhexidine; D005630:Fructans; D006602:Hexosyltransferases; D006882:Hydroxyapatites; C021451:inulosucrase; D001426:Bacterial Proteins