title: High expression of B-cell receptor inducible gene BIC in all subtypes of Hodgkin lymphoma.
abstract: In a search for genes specifically expressed in Reed-Sternberg (RS) cells of Hodgkin lymphoma (HL), we applied the serial analysis of gene expression (SAGE) technique on the HL-derived cell line DEV. Genes highly expressed in DEV were subjected to an RT-PCR analysis to confirm the SAGE results. For one of the genes, a high expression was observed in DEV and other HL-derived cell lines but not in non-Hodgkin lymphoma (NHL)-derived cell lines and normal controls, suggesting an HL-specific expression. This gene corresponds to the human BIC gene, a member of the noncoding mRNA-like molecules. RNA in situ hybridization (ISH) indicated an exclusive nucleolar localization of BIC transcripts in all RS cells in 91% of HL cases, including nodular lymphocyte predominance (NLP) HL and classical HL. Analyses of normal human tissues revealed BIC transcripts in only a small number of CD20-positive B-cells in lymph node and tonsil tissue, albeit at a much lower level compared to that of RS cells. BIC RT-PCR in the Burkitt lymphoma-derived cell line Ramos demonstrated a significant up-regulation upon cross-linking of the B-cell receptor (BcR). IkappaBalpha-mediated blocking of NF-kappaB translocation in Ramos did not effect the up-regulation of BIC expression upon BcR triggering, suggesting that activation of NF-kappaB is not involved in regulation of BIC expression. In summary, our data show that expression of BIC is specific for RS cells of HL. In normal tissue, BIC is expressed weakly in a minority of germinal center B cells. Expression of BIC can be modified/influenced by BcR triggering, indicating that BIC might play a role in the selection of B cells.
authors.affiliation: Pathology & Laboratory Medicine, University Medical Center Groningen, Groningen, The Netherlands. a.van.den.berg@path.azg.nl
authors.forename: Anke
authors.initials: A
authors.lastname: van den Berg
authors.forename: Bart-Jan
authors.initials: BJ
authors.lastname: Kroesen
authors.forename: Klaas
authors.initials: K
authors.lastname: Kooistra
authors.forename: Debora
authors.initials: D
authors.lastname: de Jong
authors.forename: Jane
authors.initials: J
authors.lastname: Briggs
authors.forename: Tjasso
authors.initials: T
authors.lastname: Blokzijl
authors.forename: Susan
authors.initials: S
authors.lastname: Jacobs
authors.forename: Joost
authors.initials: J
authors.lastname: Kluiver
authors.forename: Arjan
authors.initials: A
authors.lastname: Diepstra
authors.forename: Ewerton
authors.initials: E
authors.lastname: Maggio
authors.forename: Sibrand
authors.initials: S
authors.lastname: Poppema
mesh_terms: D000818:Animals; D005557:Formaldehyde / Q000378:metabolism; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D052138:Genes, Neoplasm / Q000502:physiology*; D006689:Hodgkin Disease / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D017403:In Situ Hybridization; D019169:Jurkat Cells / Q000737:chemistry / Q000378:metabolism; D016393:Lymphoma, B-Cell / Q000235:genetics / Q000473:pathology; D008224:Lymphoma, Follicular / Q000235:genetics / Q000473:pathology; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics / Q000473:pathology; D016612:Paraffin Embedding; D011947:Receptors, Antigen, B-Cell / Q000502:physiology*; D016707:Tissue Fixation; D014407:Tumor Cells, Cultured
chemical_list: D011947:Receptors, Antigen, B-Cell; D005557:Formaldehyde