title: Do oxidized fatty acids activate coagulation factor VII during post-prandial lipemia in women?
abstract: Factor VII is activated to VIIa within hours after dietary fat, irrespective of its fatty acid composition. Edible oils contain oxidized material (hydroxy fatty acids, HOFA). Twenty-five fasting women, aged 38 (10) years, consumed 30 g walnut oil containing 26 (6) mg HOFA. Blood was collected 2-hourly to measure plasma triglycerides and plasma lipid HOFA by gas chromatography/mass spectrometry. VII and sTF-dependent VIIa were quantified at 0, 6 and 24 h. Increased plasma triglycerides and HOFA (areas under the curve 0-8 h, AUC) were related r = 0.83, p < 0.001. VIIa increased from 2.6 (1.4) to 4.2 (1.9) ng/mL at 6 h (p < 0.001). Plasma VII remained constant. VIIa (6 h) was related to plasma triglycerides- and HOFA-AUC: r = 0.38 and 0.53, respectively (both p < 0.05). Plasma VIIa was also related to body weight, fasting triglycerides, HOFA and VII. Only HOFA-AUC and body weight related to VIIa (6 h) in stepwise regression analysis (p = 0.007 and 0.038, respectively). Oxidized, not normal, fat activates VII and could increase coronary risk in humans.
authors.affiliation: Cardiovascular Research, University of Edinburgh, George Square, Edinburgh EH8 9XF, UK.
authors.forename: Robert
authors.initials: R
authors.lastname: Wilson
authors.forename: Karin
authors.initials: K
authors.lastname: Lyall
authors.forename: E Margaret
authors.initials: EM
authors.lastname: Millar
authors.forename: Louise
authors.initials: L
authors.lastname: Smyth
authors.forename: Claire
authors.initials: C
authors.lastname: Pearson
authors.forename: Rudolph A
authors.initials: RA
authors.lastname: Riemersma
mesh_terms: D000328:Adult; D005167:Factor VII / Q000737:chemistry*; D015942:Factor VIIa / Q000737:chemistry; D005227:Fatty Acids / Q000378:metabolism*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D050356:Lipid Metabolism; D013058:Mass Spectrometry; D008875:Middle Aged; D010100:Oxygen / Q000378:metabolism*; D019518:Postprandial Period; D012044:Regression Analysis; D012306:Risk; D013997:Time Factors; D014280:Triglycerides / Q000097:blood
chemical_list: D005227:Fatty Acids; D014280:Triglycerides; D005167:Factor VII; D015942:Factor VIIa; D010100:Oxygen