title: Shear stress augments the enhanced adhesive phenotype of cells expressing the Pro33 isoform of integrin beta3.
abstract: Adhesion of platelets to the exposed extracellular matrix proteins at sites of vascular injury is partly regulated by the local fluid shear stress. Because the Leu33Pro (Pl(A)) polymorphism of integrin beta(3) confers only a modest increase in adhesion under static conditions, we used CHO and 293 cells expressing the Leu33 or Pro33 isoform of beta(3) in flow chamber experiments to test whether shear forces would alter the Pl(A) adhesive phenotype. We found that shear force augmented the Pro33-mediated enhanced adhesion to fibrinogen. This Pro33-dependent enhancement was aspirin-sensitive and was also observed on immobilized von Willebrand factor and cryoprecipitate, but not fibronectin. Thus, shear stress enhances the adhesive phenotype of the Pro33 cells to multiple physiologic substrates.
authors.affiliation: Department of Medicine, Baylor College of Medicine, One Baylor Plaza, BCM 286, N1319, Houston, TX 77030, USA.
authors.forename: K Vinod
authors.initials: KV
authors.lastname: Vijayan
authors.forename: Trevor C
authors.initials: TC
authors.lastname: Huang
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Aubrey
authors.initials: A
authors.lastname: Bernardo
authors.forename: Jing Fei
authors.initials: JF
authors.lastname: Dong
authors.forename: Pascal J
authors.initials: PJ
authors.lastname: Goldschmidt-Clermont
authors.forename: B Rita
authors.initials: BR
authors.lastname: Alevriadou
authors.forename: Paul F
authors.initials: PF
authors.lastname: Bray
mesh_terms: D000818:Animals; D016466:CHO Cells; D002448:Cell Adhesion / Q000502:physiology*; D002460:Cell Line; D006224:Cricetinae; D005340:Fibrinogen / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D039661:Integrin beta3 / Q000737:chemistry / Q000502:physiology*; D011392:Proline / Q000502:physiology*; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism
chemical_list: D005353:Fibronectins; D039661:Integrin beta3; D005340:Fibrinogen; D011392:Proline; D011451:Prostaglandin-Endoperoxide Synthases