The cytokine markers in Staphylococcus aureus mastitis of bovine mammary gland.
TaqMan real time PCR was used to study the transcriptional activity of the bovine IL-2, IL-6, IL-12p40, IFN-gamma, TNF-alpha and granulocyte-monocyte colony stimulating factor of whole milk cells in bovine mammary gland experimentally infected with Staphylococcus aureus. Cytokine transcriptional activity was monitored at 7, 24 and 32 h Post-infection (Pi). IL-12 and TNF-alpha levels were significantly elevated at 24 h Pi followed by sharp decrease at 32 h pi. IL-2 level was decreased at 32 h pi. IL-12 and IFN-gamma showed a significant interaction at 24 h pi. The significant elevations of the IL-12 and TNF-alpha transcriptional level most likely indicate their important role in regulation of the immune responses of bovine mammary gland in S. aureus infection. Depression of IL-2 could reflect the suppressive nature of the S. aureus mastitis.
Alluwaimi
A M
AM
Department of Microbiology and Parasitology, College of Veterinary Medicine and Animal Resources, King Faisal University, PO Box 35252, Al-AHSAA, 31982 Saudi Arabia. alluwaimi@kfu.edu.sa
Leutenegger
C M
CM
Farver
T B
TB
Rossitto
P V
PV
Smith
W L
WL
Cullor
J S
JS
D000818:Animals; D002417:Cattle; D016207:Cytokines / Q000235:genetics / Q000276:immunology*; D017931:DNA Primers; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D007371:Interferon-gamma; D018664:Interleukin-12; D053762:Interleukin-12 Subunit p40; D007376:Interleukin-2; D015850:Interleukin-6; D008414:Mastitis, Bovine / Q000276:immunology*; D008892:Milk / Q000166:cytology / Q000276:immunology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D021122:Protein Subunits; D012333:RNA, Messenger / Q000032:analysis; D013203:Staphylococcal Infections / Q000276:immunology*; D013211:Staphylococcus aureus; D014409:Tumor Necrosis Factor-alpha
D016207:Cytokines; D017931:DNA Primers; D053762:Interleukin-12 Subunit p40; D007376:Interleukin-2; D015850:Interleukin-6; D021122:Protein Subunits; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D018664:Interleukin-12; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor