title: Antioxidative effect of chitosan on chronic carbon tetrachloride induced hepatic injury in rats.
abstract: Carbon tetrachloride (CCl(4)) is a toxic material known to induce lipid peroxidation and liver damage. To determine if chitosan has antioxidative effects on CCl(4)-induced liver injury, we administered 1 ml/kg of CCl(4) resolved in a 50% corn oil solution to rats every week by intraperitoneal injection. Chitosan (200 mg/kg body weight per day, MW 380,000 Da) was administered to the CCl(4) + chitosan treated rats by oral gavage during the experimental period. Chitosan significantly decreased liver thiobarbituric acid reactive substances (TBARS) and increased antioxidant enzyme activities (catalase and superoxide dismutase (SOD)). Fatty acid composition was not remarkably changed by chitosan; only arachidonic acid (20:4n-6) levels were significantly altered by CCl(4). Chitosan administration in the present experiment did not restore the decreased delta5-desaturase activity. In addition, chitosan supplementation did not prevent the CCl(4) induced degradation of CYP2E1. In conclusion, our results suggest that chitosan has antioxidative but not detoxifying effects on chronic CCl(4) induced hepatic injury in rats.
authors.affiliation: Department of Applied Biology and Chemistry, Konkuk University, 1 Hwayang-dong, Kwanghin-gu, Seoul 143-701, South Korea.
authors.forename: Tae Il
authors.initials: TI
authors.lastname: Jeon
authors.forename: Seong Gu
authors.initials: SG
authors.lastname: Hwang
authors.forename: Nam Gyu
authors.initials: NG
authors.lastname: Park
authors.forename: Yu Ri
authors.initials: YR
authors.lastname: Jung
authors.forename: Soo Im
authors.initials: SI
authors.lastname: Shin
authors.forename: Sung Don
authors.initials: SD
authors.lastname: Choi
authors.forename: Dong Ki
authors.initials: DK
authors.lastname: Park
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000627:therapeutic use*; D001835:Body Weight / Q000187:drug effects; D002252:Carbon Tetrachloride Poisoning / Q000188:drug therapy* / Q000201:enzymology / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002686:Chitin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000627:therapeutic use*; D048271:Chitosan; D019392:Cytochrome P-450 CYP2E1 / Q000096:biosynthesis; D004195:Disease Models, Animal; D007274:Injections, Intraperitoneal; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
chemical_list: D000975:Antioxidants; D017392:Thiobarbituric Acid Reactive Substances; D002686:Chitin; D048271:Chitosan; D002374:Catalase; D019392:Cytochrome P-450 CYP2E1