Weight loss in tumour-bearing mice is not associated with changes in resistin gene expression in white adipose tissue.
Resistin, a product of white adipose tissue, is postulated to induce insulin resistance in obesity and regulate adipocyte differentiation. The aim of this study was to examine resistin gene expression in adipose tissue from mice bearing the MAC16 adenocarcinoma, which induces cancer cachexia with marked wasting of adipose tissue and skeletal muscle mass. MAC16-bearing mice lost weight progressively over the period following tumour transplantation, while the weight of control mice remained stable. Leptin mRNA in gonadal fat was 50 % lower in MAC16 mice than in controls (p < 0.05). Plasma insulin concentrations were also significantly lower in the MAC16 group (p < 0.05). However, resistin mRNA level in gonadal fat in MAC16 mice was similar to controls (94 % of controls). Thus, despite severe weight loss and significant falls in leptin expression and insulin concentration, resistin gene expression appears unchanged in white adipose tissue of mice with MAC16 tumour. Maintenance of resistin production may help inhibit the formation of new adipocytes in cancer cachexia.
Gómez-Ambrosi
J
J
Neuroendocrine and Obesity Biology Unit, Department of Medicine, University of Liverpool, University Clinical Departments, UK. jagomez@unav.es
Zabalegui
N
N
Bing
C
C
Tisdale
M J
MJ
Trayhurn
P
P
Williams
G
G
D000230:Adenocarcinoma / Q000150:complications / Q000378:metabolism*; D000273:Adipose Tissue / Q000503:physiopathology*; D000818:Animals; D002100:Cachexia / Q000235:genetics* / Q000503:physiopathology; D004195:Disease Models, Animal; D005260:Female; D005786:Gene Expression Regulation; D006729:Hormones, Ectopic / Q000235:genetics*; D007328:Insulin / Q000097:blood; D036341:Intercellular Signaling Peptides and Proteins; D051379:Mice; D008815:Mice, Inbred Strains; D009368:Neoplasm Transplantation; D020932:Nerve Growth Factor; D011506:Proteins; D012333:RNA, Messenger / Q000032:analysis; D052243:Resistin; D015431:Weight Loss / Q000235:genetics*
D006729:Hormones, Ectopic; D007328:Insulin; D036341:Intercellular Signaling Peptides and Proteins; D011506:Proteins; D012333:RNA, Messenger; D052243:Resistin; C499623:Retn protein, mouse; C413576:Retnla protein, mouse; D020932:Nerve Growth Factor