[Expression of human G6PD gene in K562 cells mediated by retroviral vector].
OBJECTIVE
This study aimed to investigate the feasibility of gene therapy for severe G6PD deficiency.


METHODS
The recombinant retroviral vector bearing normal human G6PD cDNA was constructed and transferred into the erythroleukemia cell line K562. Author identified the integration of NeoR gene in the targeted cellular DNA by means of specific PCR. Quantitative method was used to measure the expression of G6PD in the targeted cells.


RESULTS
Construction of the recombinant retroviral vector was successfully established. PCR indicated the integration of NeoR gene in the targeted genomic DNA of the cells. The vector was also shown to be capable of expressing the foreign gene compared to the control (P<0.01).


CONCLUSIONS
The recombinant retroviral vector is competent for transferring and expressing the G6PD gene.
Zhou
Ling
L
Department of Hematology, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China.
Guo
Kunyuan
K
Li
Jiangqi
J
D015870:Gene Expression; D015316:Genetic Therapy; D005822:Genetic Vectors; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics*; D005955:Glucosephosphate Dehydrogenase Deficiency / Q000628:therapy; D006801:Humans; D020014:K562 Cells; D012190:Retroviridae / Q000235:genetics*; D014162:Transfection
D005954:Glucosephosphate Dehydrogenase