title: IL-11 up-regulates Tie-2 expression during the healing of gastric ulcers in rats.
abstract: OBJECTIVE
  To investigate Tie-2 expression during the repair of acetic acid-induced gastric ulcers in rats treated with recombinant human IL-11 (rhIL-11) and in untreated control animals.
  
  
  METHODS
  Gastric ulcers were induced in male Wistar rats by applying acetic acid to the fundus of the stomach. RhIL-11 (100 microg/kg twice daily, subcutaneously) was administered from two days before ulcer induction and continued for five days after the induction. Control rats received bovine serum albumin. Gastric specimens were collected at 3 and 5 days after the induction of ulcer for immunohistochemical observation, Western blotting, and reverse transcription polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  Immunohistochemical and Western blot analysis demonstrated that Tie-2 expression was enhanced in the rhIL-11-treated rats compared with the control animals at both intervals.
  
  
  CONCLUSIONS
  These findings suggested that IL-11 could accelerate ulcer healing, in part, by up-regulating Tie-2 expression and promoting angiogenesis.
authors.affiliation: Department of Molecular Pathology, Atomic Bomb Disease Institute, Nagasaki University School of Medicine, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan. cywen518@net.nagasaki-u.ac.jp
authors.forename: Chun-Yang
authors.initials: CY
authors.lastname: Wen
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ito
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Long-Dian
authors.initials: LD
authors.lastname: Chen
authors.forename: Zhao-Min
authors.initials: ZM
authors.lastname: Xu
authors.forename: Mutsumi
authors.initials: M
authors.lastname: Matsuu
authors.forename: Kazuko
authors.initials: K
authors.lastname: Shichijo
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Nakayama
authors.forename: Masahiro
authors.initials: M
authors.lastname: Nakashima
authors.forename: Ichiro
authors.initials: I
authors.lastname: Sekine
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000494:pharmacology*; D008297:Male; D051381:Rats; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics*; D042787:Receptor, TIE-2; D011994:Recombinant Proteins / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013276:Stomach Ulcer / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D007375:Interleukin-1; D011994:Recombinant Proteins; D020794:Receptor Protein-Tyrosine Kinases; D042787:Receptor, TIE-2