title: Phospholipid chlorohydrins cause ATP depletion and toxicity in human myeloid cells.
abstract: Chlorohydrins of stearoyl-oleoyl phosphatidylcholine (SOPC), stearoyl-linoleoyl phosphatidylcholine, and stearoyl-arachidonyl phosphatidylcholine were incubated with cultured myeloid cells (HL60) for 24 h, and the cellular ATP level was measured using a bioluminescent assay. The chlorohydrins caused significant depletion of cellular ATP in the range 10-100 microM. The ATP depletion by the phospholipid chlorohydrins was slightly less than that of 4-hydroxy-2-nonenal, but greater than that of hexanal, trans-2-nonenal, and autoxidised palmitoyl-arachidonoyl phosphatidylcholine. SOPC chlorohydrin was also found to cause loss of viability in U937 cells, and thus phospholipid chlorohydrins could contribute to the formation of a necrotic core in advanced atherosclerotic lesions.
authors.affiliation: Department of Bioscience, University of Strathclyde, 204 George Street, Glasgow G1 1XW, UK.
authors.forename: Gary
authors.initials: G
authors.lastname: Dever
authors.forename: Laura-Jayne
authors.initials: LJ
authors.lastname: Stewart
authors.forename: Andrew R
authors.initials: AR
authors.lastname: Pitt
authors.forename: Corinne M
authors.initials: CM
authors.lastname: Spickett
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D002728:Chlorohydrins / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D049449:Luminescence; D010743:Phospholipids / Q000494:pharmacology*; D021241:Spectrometry, Mass, Electrospray Ionization; D020298:U937 Cells
chemical_list: D002728:Chlorohydrins; D010743:Phospholipids; D000255:Adenosine Triphosphate