Characterization of the F gene of contemporary mumps virus strains isolated in Japan.
Mumps virus (MuV) strains isolated in Saitama Prefecture, Japan, from 1997 to 2001, were examined by analyzing the SH and the F gene nucleotide sequences. The results of the SH gene analysis showed that only genotype G was found in 2001 as well as in 2000, and that genotype J, which we proposed as a new genotype in a previous study, was from a different lineage than the genotype J described by Tecle et al. (J. Gen. Virol. 82, 2675-2680). We therefore, propose to rename the genotype as K to avoid confusion. Then, the F gene of genotypes G, H, and K strains were analyzed together with previously reported strains in this study. The results of phylogenetic analysis of the F gene nucleotide sequences showed that these strains formed a cluster as described by the SH gene analysis. Alignment of the F amino acid sequences showed that the F protein was well conserved among strains of different genotypes with a few amino acid differences. These results provide better information for the characterization of contemporary MuV strains in Japan.
Uchida
Kazue
K
Virus Division, Saitama Institute of Public Health, Saitama, Saitama 338-0824, Japan. a0183437@pref.saitama.jp
Shinohara
Michiyo
M
Shimada
Shin-ichi
S
Segawa
Yukari
Y
Kimura
Kazuhiro
K
Hoshino
Yoji
Y
D000595:Amino Acid Sequence; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005838:Genotype; D006801:Humans; D007564:Japan; D008297:Male; D008969:Molecular Sequence Data; D009107:Mumps / Q000821:virology*; D009109:Mumps virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014760:Viral Fusion Proteins / Q000737:chemistry / Q000235:genetics*; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
D004279:DNA, Viral; D014760:Viral Fusion Proteins; D014764:Viral Proteins; C067386:small hydrophobic protein, Mumps virus