Severe DNA damage in K-ras gene patterns of PCR products in mice fed a 1% protein diet and exposed to a high dose of radiation.
K-ras gene patterns of PCR products in response to total body irradiation (TBI) in mice fed four kinds of diets were assessed. Male mice were fed a 1% protein diet, 7% protein diet, 20% protein diet, or a 60% protein diet for 4 weeks, received TBI at a dose of 0, 2, or 6 Gy, and were sacrificed 4 weeks post-TBI. Severe DNA damage in mice fed a 1% protein diet with a high dose of radiation exposure (TBI at 6 Gy) was observed.
Shin
Sung Jae
SJ
Department of Food Science Research for Health, National Institute of Health and Nutrition, Tokyo, Japan. mhjrm@nih.go.jp
D000821:Animal Feed; D000818:Animals; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D004249:DNA Damage / Q000187:drug effects* / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005609:Free Radicals; D011905:Genes, ras; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D016133:Polymerase Chain Reaction / Q000379:methods; D017382:Reactive Oxygen Species; D013997:Time Factors; D014916:Whole-Body Irradiation
D005609:Free Radicals; D017382:Reactive Oxygen Species; D004247:DNA