title: Evaluation of anthraquinone-2-sulfonyl chloride for determination of phenol in water by liquid chromatography using pre-column phase-transfer catalysed derivatization.
abstract: A new reagent, anthraquinone-2-sulfonyl chloride (ASC), has been used for the derivatization of phenols. Several compounds with different polarity were selected to evaluate the new reagent and derivatives of these phenols prepared via a facile pathway. The optimal conditions for analytical derivatization and mechanism of the derivatization reaction are discussed. The derivatization procedure involves an ion-pair extraction of the deprotonated phenols with a tetrabutylammonium counter ion to an organic phase. At the interface of two phases, the derivatization reaction occurs quantitatively at room temperature within 3 min. The derivatives are stable and readily amenable to analysis by normal-phase and reversed-phase high performance liquid chromatography (HPLC). Excellent linearity response was demonstrated over the concentration range of 0.2 mumol/L to 200 mumol/L at 320 nm for normal-phase HPLC, at 256 nm for reversed-phase HPLC. Combined with preconcentration using a Waters Sep-Pak Plus C18 cartridge, detection limits of phenols for water sample analysis were as low as 1 x 10(-9) mol/L (ca. 0.1 microgram/L).
authors.affiliation: Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China. fengfang1@hotmail.com
authors.forename: Fang
authors.initials: F
authors.lastname: Feng
authors.forename: Bunji
authors.initials: B
authors.lastname: Uno
authors.forename: Massashi
authors.initials: M
authors.lastname: Goto
authors.forename: Zheng-xing
authors.initials: ZX
authors.lastname: Zhang
authors.forename: Deng-kui
authors.initials: DK
authors.lastname: An
mesh_terms: D000880:Anthraquinones; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D005069:Evaluation Studies as Topic; D007202:Indicators and Reagents; D010636:Phenols / Q000032:analysis*; D012997:Solvents; D014874:Water Pollutants, Chemical / Q000032:analysis*; D014881:Water Supply / Q000032:analysis*
chemical_list: D000880:Anthraquinones; D007202:Indicators and Reagents; D010636:Phenols; D012997:Solvents; D014874:Water Pollutants, Chemical; C023554:anthraquinone sulfonate