title: [Tissue engineering: new treatment of cartilage alterations in degenerative joint diseases in horses--preliminary results of a long term study].
abstract: Degenerative alterations in fetlock joints of the forelimb are common diagnoses for horses. The hyaline cartilage has a low capacity to regenerate and the treatment by veterinarians is often insufficient. As a final result, horses with articular cartilage defects are often not able to take part in competitions anymore. To establish an autologous cartilage repair method, we set artificial lesions (8 mm in diameter) into the fetlock joints of the forelimb of three horses. These defects were closed with autologous chondrocyte implants, which were fixed with titan-suture-anchors. After 3, 12 and 24 months, biopsies were taken by arthroscopy. One horse was euthanized after 9, another one after 24 months. The repair tissue was examined histologically and by biochemical analysis of hydroxyproline and glycosaminoglycan, which are typical cartilage related substances. After 9 months, the integration of the implant into native cartilage was demonstrated by electron microscopy. After 24 months, histological staining showed a similar morphology of the cartilage repair tissue compared with the surrounding native cartilage. Biochemical analysis of typical cartilage matrix molecules revealed formation of hyaline-like cartilage within tissue engineered autologous chondrocyte transplants.
authors.affiliation: Forschungszentrum für Medizintechnik und Biotechnologie e.V. (FZMB), Bad Langensalza. Dbarnewitz@fzmb.de
authors.forename: D
authors.initials: D
authors.lastname: Barnewitz
authors.forename: A
authors.initials: A
authors.lastname: Evers
authors.forename: J
authors.initials: J
authors.lastname: Zimmermann
authors.forename: I
authors.initials: I
authors.lastname: Wilke
authors.forename: C
authors.initials: C
authors.lastname: Kaps
authors.forename: M
authors.initials: M
authors.lastname: Sittinger
mesh_terms: D000818:Animals; D001182:Arthroscopy / Q000662:veterinary; D002357:Cartilage Diseases / Q000473:pathology / Q000628:therapy / Q000662:veterinary*; D002358:Cartilage, Articular / Q000473:pathology*; D005552:Forelimb; D006734:Horse Diseases / Q000473:pathology / Q000628:therapy*; D006736:Horses; D010003:Osteoarthritis / Q000473:pathology / Q000628:therapy / Q000662:veterinary*; D023822:Tissue Engineering / Q000662:veterinary*; D014182:Transplantation, Autologous / Q000662:veterinary