title: [Synthesis and properties of oligodeoxyribonucleotids with mono- and diphosphoryldisulfide internucleotide groupings].
abstract: The synthesis of oligodeoxyribonucleotides bearing mono- and diphosphoryldisulfide internucleotide links was optimized. Oligonucleotide 3'-thiophosphorothioates were modified using the thiophosphoryl-disulfide exchange with preactivated 5'-deoxy-5'-mercaptooligonucleotides or 5'-phosphorothioate derivatives both with and without a complementary template. The lack of template was shown to differently affect the product ratio (homo- and heterodimers) in the reactions of mono- and diphosphoryldisulfide-containing oligonucleotides. A replacement of one natural phosphodiester bond in 15-16-mer duplexes by a mono- or diphosphoryldisulfide group causes a slight thermal destabilization of the corresponding duplex. The disulfide recombination of the resulting compounds was studied.
authors.affiliation: Moscow State University, Chemical Faculty, Vorob'evy gory, GSP-3 Moscow, 119899 Russia. metelev@belozersky.msu.ru
authors.forename: V G
authors.initials: VG
authors.lastname: Metelev
authors.forename: E A
authors.initials: EA
authors.lastname: Kubareva
authors.forename: E A
authors.initials: EA
authors.lastname: Romanova
authors.forename: T S
authors.initials: TS
authors.lastname: Oretskaia
mesh_terms: D001483:Base Sequence; D004220:Disulfides / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D004220:Disulfides; D009838:Oligodeoxyribonucleotides