title: Histopathological study of dental pulp tissue capped with enamel matrix derivative.
abstract: The purpose of this study was to examine the histopathological response of dental pulp tissue to enamel matrix derivative (EMD) used as a pulp capping material. Thirty-two teeth from two mongrel dogs were divided into four equal groups. One group served as controls, and the others were used for deep Class V cavity preparation followed by direct pulp capping with enamel matrix derivative. The treated teeth were extracted after 1, 4, and 8 weeks and prepared for histopathological examination by light microscopy. All teeth prepared after 4 and 8 weeks demonstrated an increase in tertiary dentin, suggesting that enamel matrix derivative exerts a considerable influence on odontoblasts and endothelial cells of capillaries in dental pulp tissue. These results imply that enamel matrix derivative used as a pulp capping material may play a role in the calcification of dental pulp tissue.
authors.affiliation: Department of Endodontics, Showa University School of Dentistry, Tokyo, Japan.
authors.forename: Nelson Tatsunari
authors.initials: NT
authors.lastname: Ishizaki
authors.forename: Koukichi
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Kimura
authors.forename: Xiaogu
authors.initials: X
authors.lastname: Wang
authors.forename: Akinori
authors.initials: A
authors.lastname: Yamashita
mesh_terms: D000818:Animals; D002113:Calcification, Physiologic / Q000187:drug effects; D002196:Capillaries / Q000187:drug effects / Q000473:pathology; D003723:Dental Amalgam; D003736:Dental Cavity Lining; D003737:Dental Cavity Preparation / Q000145:classification; D003746:Dental Enamel Proteins / Q000627:therapeutic use*; D003782:Dental Pulp / Q000187:drug effects / Q000473:pathology*; D003785:Dental Pulp Capping; D003789:Dental Pulp Exposure / Q000628:therapy; D003793:Dental Restoration, Permanent; D003809:Dentin, Secondary / Q000187:drug effects / Q000473:pathology; D004285:Dogs; D004730:Endothelium, Vascular / Q000187:drug effects / Q000473:pathology; D009804:Odontoblasts / Q000187:drug effects / Q000473:pathology; D011897:Random Allocation; D013997:Time Factors; D015035:Zinc Oxide-Eugenol Cement
chemical_list: D003746:Dental Enamel Proteins; D015035:Zinc Oxide-Eugenol Cement; C109069:enamel matrix proteins; D003723:Dental Amalgam