Protein separations using colloidal magnetic nanoparticles.
Phospholipid-coated colloidal magnetic nanoparticles with mean magnetite core size of 8 nm are shown to be effective ion exchange media for the recovery and separation of proteins from protein mixtures. These particles have high adsorptive capacities (up to 1200 mg protein/mL adsorbent, an order of magnitude larger than the best commercially available adsorbents) and exhibit none of the diffusional resistances offered by conventional porous ion exchange media. Protein-laden particles are readily recovered from the feed solution using high-gradient magnetic filtration.
Bucak
Seyda
S
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Jones
Deverraux A
DA
Laibinis
Paul E
PE
Hatton
T Alan
TA
D000327:Adsorption; D002852:Chromatography, Ion Exchange / Q000379:methods*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry*; D003102:Colloids; D045304:Cytochromes c / Q000737:chemistry / Q000302:isolation & purification; D008081:Liposomes / Q000302:isolation & purification*; D008280:Magnetics; D008863:Microspheres; D036103:Nanotechnology / Q000379:methods*; D010743:Phospholipids / Q000737:chemistry; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D014462:Ultrafiltration / Q000379:methods*
D020099:Coated Materials, Biocompatible; D003102:Colloids; D008081:Liposomes; D010743:Phospholipids; D011506:Proteins; D045304:Cytochromes c