Binding of alpha2-macroglobulin to collagen type I: modification of collagen matrix by alpha2-macroglobulin induces the enhancement of macrophage migration.
Alpha2-macroglobulin (a2M) secreted by tissue macrophages and fibroblasts functions in the environment of extracellular matrix macromolecules. We supposed that it may interact with these molecules and change the properties of extracellular matrix. Modified variant of ELISA was used to prove the direct binding of human a2M to collagen. Native and transformed by plasmin a2M, as well as plasmin, used as the control, were labeled by biotin. It has been found that the transformed, but not the native a2M form binds to type I collagen molecules: K(d)=(1.168 +/- 1.14) x 10(-11) M. The data obtained give a strong evidence of high power of the interaction between a2M and type I collagen: practically no reverse dissociation may be seen for such a binding. The modification of three-dimensional collagen matrix by binding to the transformed a2M resulted in the enhancement of migration of macrophages, carrying the receptors for a2M, but not splenocytes that lack for such receptors. Our results allow to suggest that a2M may be one of the components of extracellular matrix, and may change the properties of microenvironment for immunocompetent cells during the processes of inflammation, reparation and tumor invasion.
Larin
Sergei S
SS
Russian State Medical University, Moscow, Russia.
Gorlina
Natalia K
NK
Kozlov
Ivan G
IG
Cheredeev
Anatoly N
AN
Zorin
Nikolai A
NA
Zorina
Raisa M
RM
D002465:Cell Movement / Q000276:immunology*; D018914:Cells, Immobilized / Q000276:immunology; D024042:Collagen Type I / Q000276:immunology* / Q000378:metabolism; D005109:Extracellular Matrix / Q000276:immunology / Q000378:metabolism; D005341:Fibrinolysin / Q000378:metabolism; D006801:Humans; D008262:Macrophage Activation / Q000276:immunology; D008264:Macrophages / Q000166:cytology / Q000276:immunology* / Q000502:physiology; D011485:Protein Binding; D013154:Spleen / Q000166:cytology / Q000276:immunology; D000511:alpha-Macroglobulins / Q000276:immunology* / Q000378:metabolism
D024042:Collagen Type I; D000511:alpha-Macroglobulins; D005341:Fibrinolysin