Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for mtDNA typing in hokkaido brown bear (Ursus arctos yesoensis).
To develop an easy method of typing of mitochondrial DNA (mtDNA) in Hokkaido brown bears (Ursus arctos yesoensis), the PCR-RFLP technique was improved using four restriction enzymes: Mbo 1, Cfr 13 I, TspE 1, and Fok 1. This approach identified seven groups of mtDNA haplotypes, HB1/2/5-7, HB 3, HB4, HB8/9, HB10/11, HB12 and HB13 from 102 brown bears of northern, central and eastern Hokkaido.
Satoh
Yuka
Y
Laboratory of Wildlife Biology, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Mano
Tsutomu
T
Tsuruga
Hifumi
H
Masuda
Ryuichi
R
Matsuhashi
Tamako
T
Onuma
Manabu
M
Suzuki
Masatsugu
M
Ohtaishi
Noriyuki
N
D000818:Animals; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics*; D006239:Haplotypes; D007564:Japan; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D001503:Ursidae / Q000235:genetics*
D004272:DNA, Mitochondrial