The effect of tetracycline hydrochloride on the attachment of gingival fibroblasts. A scanning electron microscopic study on the effect of ultrasonic scaling before root conditioning.
Twenty four human teeth, extracted due to severe periodontitis were used in the present Following extraction, the border between healthy and diseased was marked with a small dental bur. The portions of diseased roots were either untreated or were treated with ultrasonic scaling or tetracycline hydrochloride, or ultrasonic scaling followed by tetracycline hydrochloride. Human gingival fibroblasts were then added to the roots so treated and were allowed to incubate for 72 h and examined by scanning electron microscopy. There were a significantly greater number of fibroblasts attached to specimens treated with ultrasonic scaling and ultrasonic scaling followed by root demineralization. No cells were could be seen on the untreated surfaces whether or not root surface conditioning was used.
Babay
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Department Preventive Dentistry, College of Dentistry, King Saud University, Riyadh, Saudi Arabia, 11545.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects*; D012534:Dental Scaling; D005347:Fibroblasts / Q000187:drug effects; D005881:Gingiva / Q000166:cytology* / Q000187:drug effects*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010518:Periodontitis / Q000628:therapy; D011897:Random Allocation; D016369:Smear Layer; D013752:Tetracycline / Q000494:pharmacology*; D014092:Tooth Root / Q000187:drug effects*; D014464:Ultrasonic Therapy
D000900:Anti-Bacterial Agents; D013752:Tetracycline