title: Cell wall attachment of a widely distributed peptidoglycan binding domain is hindered by cell wall constituents.
abstract: The C-terminal region (cA) of the major autolysin AcmA of Lactococcus lactis contains three highly similar repeated regions of 45 amino acid residues (LysM domains), which are separated by nonhomologous sequences. The cA domain could be deleted without destroying the cell wall-hydrolyzing activity of the enzyme in vitro. This AcmA derivative was capable neither of binding to lactococcal cells nor of lysing these cells while separation of the producer cells was incomplete. The cA domain and a chimeric protein consisting of cA fused to the C terminus of MSA2, a malaria parasite surface antigen, bound to lactococcal cells specifically via cA. The fusion protein also bound to many other Gram-positive bacteria. By chemical treatment of purified cell walls of L. lactis and Bacillus subtilis, peptidoglycan was identified as the cell wall component interacting with cA. Immunofluorescence studies showed that binding is on specific locations on the surface of L. lactis, Enterococcus faecalis, Streptococcus thermophilus, B. subtilis, Lactobacillus sake, and Lactobacillus casei cells. Based on these studies, we propose that LysM-type repeats bind to peptidoglycan and that binding is hindered by other cell wall constituents, resulting in localized binding of AcmA. Lipoteichoic acid is a candidate hindering component. For L. lactis SK110, it is shown that lipoteichoic acids are not uniformly distributed over the cell surface and are mainly present at sites where no MSA2cA binding is observed.
authors.affiliation: Department of Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands.
authors.forename: Anton
authors.initials: A
authors.lastname: Steen
authors.forename: Girbe
authors.initials: G
authors.lastname: Buist
authors.forename: Kees J
authors.initials: KJ
authors.lastname: Leenhouts
authors.forename: Mohamed
authors.initials: M
authors.lastname: El Khattabi
authors.forename: Froukje
authors.initials: F
authors.lastname: Grijpstra
authors.forename: Aldert L
authors.initials: AL
authors.lastname: Zomer
authors.forename: Gerard
authors.initials: G
authors.lastname: Venema
authors.forename: Oscar P
authors.initials: OP
authors.lastname: Kuipers
authors.forename: Jan
authors.initials: J
authors.lastname: Kok
mesh_terms: D001412:Bacillus subtilis / Q000737:chemistry / Q000648:ultrastructure; D001665:Binding Sites; D002473:Cell Wall / Q000737:chemistry* / Q000378:metabolism; D013293:Enterococcus faecalis / Q000737:chemistry / Q000648:ultrastructure; D006094:Gram-Positive Bacteria / Q000737:chemistry* / Q000648:ultrastructure; D007778:Lactobacillus / Q000737:chemistry / Q000648:ultrastructure; D013294:Lactococcus lactis / Q000737:chemistry / Q000648:ultrastructure; D009113:Muramidase / Q000378:metabolism; D010457:Peptidoglycan / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012091:Repetitive Sequences, Nucleic Acid; D013291:Streptococcus / Q000737:chemistry / Q000648:ultrastructure
chemical_list: D010457:Peptidoglycan; C092497:AcmA protein, Lactococcus lactis; D009113:Muramidase