title: Expression of heat shock and cold shock proteins in the gorgonian Leptogorgia virgulata.
abstract: In this study, we analyzed the response of the temperate, shallow-water gorgonian, Leptogorgia virgulata, to temperature stress. Proteins were pulse labeled with (35)S-methionine/cysteine for 1 h to 2 h at 22 degrees C (control), or 38 degrees C, or for 4 h at 12.5 degrees C. Heat shock induced synthesis of unique proteins of 112, 89, and 74 kDa, with 102, 98 and 56 kDa proteins present in the control as well. Cold shock from 22 degrees C-12.5 degrees C induced the synthesis of a 25 kDa protein, with a 44 kDa protein present in the control as well. Control samples expressed unique proteins of 38, and 33 kDa. Non-radioactive proteins expressed under the same conditions as above, as well as natural field conditions, were tested for reactivity with antibodies to heat shock proteins (HSPs). HSP60 was the major protein found in L. virgulata. Although HSP47, HSP60, and HSP104 were present in all samples, the expression of HSP60 was enhanced in heat stressed colonies, while HSP47 and HSP104 expression were greatest in cold shocked samples. Inducible HSP70 was expressed in cold-shocked, heat-shocked, and field samples. Constitutively expressed HSP70 was absent from all samples. The expression of HSP90 was limited to heat shocked colonies. The expression of both HSP70 and HSP104 suggests that the organism may also develop a stress tolerance response.
authors.affiliation: Department of Biology, University of Richmond, Virginia 23173, USA. kingsle@richmond.edu
authors.forename: Roni J
authors.initials: RJ
authors.lastname: Kingsley
authors.forename: Evelyn
authors.initials: E
authors.lastname: Afif
authors.forename: Brandon C
authors.initials: BC
authors.lastname: Cox
authors.forename: Sonali
authors.initials: S
authors.lastname: Kothari
authors.forename: Karmon
authors.initials: K
authors.lastname: Kriechbaum
authors.forename: Katherine
authors.initials: K
authors.lastname: Kuchinsky
authors.forename: Anna T
authors.initials: AT
authors.lastname: Neill
authors.forename: Anita F
authors.initials: AF
authors.lastname: Puri
authors.forename: Valerie M
authors.initials: VM
authors.lastname: Kish
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D015153:Blotting, Western; D003063:Cnidaria / Q000378:metabolism*; D003080:Cold Temperature; D005786:Gene Expression Regulation; D006360:Heat-Shock Proteins / Q000032:analysis / Q000378:metabolism*; D006358:Hot Temperature; D013312:Stress, Physiological / Q000503:physiopathology*
chemical_list: D006360:Heat-Shock Proteins