title: Oscillapeptin J, a new grazer toxin of the freshwater cyanobacterium Planktothrix rubescens.
abstract: Oscillapeptin J (1), a new and highly potent crustacean grazer toxin, was isolated from the axenic cyanobacterium Planktothrix rubescens, which frequently forms blooms in freshwater lakes. Chemical and spectroscopic analyses, including high resolving MS and two-dimensional NMR, were used to elucidate the compound's structure as a depsiheptapeptide of the oscillapeptin type. Strict application of a bioassay-guided isolation procedure proved this compound to be one of the major causative agents (besides [d-Asp(3),(E)-Dhb(7)]microcystin-RR) of the acute grazer toxicity of P. rubescens from Lake Zürich. The LC(50) value of oscillapeptin J as determined for the freshwater crustacean Thamnocephalus platyurus was 15.6 microM.
authors.affiliation: Limnological Station, Institute of Plant Biology, University of Zürich, Seestrasse 187, 8802 Kilchberg, Switzerland. roderich.suessmuth@uni-tuebingen.de
authors.forename: Judith F
authors.initials: JF
authors.lastname: Blom
authors.forename: Bojan
authors.initials: B
authors.lastname: Bister
authors.forename: Daniel
authors.initials: D
authors.lastname: Bischoff
authors.forename: Graeme
authors.initials: G
authors.lastname: Nicholson
authors.forename: Günther
authors.initials: G
authors.lastname: Jung
authors.forename: Roderich D
authors.initials: RD
authors.lastname: Süssmuth
authors.forename: Friedrich
authors.initials: F
authors.lastname: Jüttner
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity; D000458:Cyanobacteria / Q000737:chemistry*; D033364:Decapoda / Q000187:drug effects*; D005858:Germany; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity
chemical_list: D001427:Bacterial Toxins; D010456:Peptides, Cyclic; C476749:oscillapeptin J