title: Importance of Protocol Immunization in Epitope Selectivity of Monoclonal Antibodies Interacting with Binding Subunit of Viscumin.
abstract: The application of two immunization protocols and two screening systems has allowed to produce five hybridomas mlb5, mlb6, mlb7, mlb9 and Mbch1, secreting mAbs against different sites of viscumin B-subunit. On the base of mlb9 and Mbch1 hybridomas, the test-system has been developed, able to detect up to 5 ng/ml of viscumin and up to 1 ng/ml of its B-chain. Produced hybridomas and monoclonal antibodies will be used for the studies of intracellular transport of plant toxins. Monoclonal antibody mlb7 will be used for the studies of viscumin interactions with immune system of mammals.
authors.affiliation: M.V. Lomonosov Moscow State University, Moscow, Russia.
authors.forename: Mikhail M.
authors.initials: MM
authors.lastname: Moisenovich
authors.forename: Svetlana G.
authors.initials: SG
authors.lastname: Egorova
authors.forename: Olga V.
authors.initials: OV
authors.lastname: Chelnokova
authors.forename: Irina A.
authors.initials: IA
authors.lastname: Demina
authors.forename: Elena R.
authors.initials: ER
authors.lastname: Polosukhina
authors.forename: Natalia V.
authors.initials: NV
authors.lastname: Kozlovskaya
authors.forename: Ekaterina N.
authors.initials: EN
authors.lastname: Popova
authors.forename: Gulnar V.
authors.initials: GV
authors.lastname: Fattakhova
authors.forename: Olga N.
authors.initials: ON
authors.lastname: Solopova
authors.forename: Igor I.
authors.initials: II
authors.lastname: Agapov