title: Early detection in saliva of polypeptides associated to isoproterenol-induced mouse parotid hypertrophy.
abstract: Chronic administration of isoproterenol (IPR) results in a marked hypertrophy and in the induction of a group of putative proline-rich polypeptides in the mouse parotid glands. Some of these polypeptides (pps C-G) have been considered as molecular markers of the parotid gland enlargement. Given the secretory character of polypeptides C-G, the polypeptide composition of mouse saliva was used to monitor the IPR-induced salivary gland hypertrophy. Whole saliva was collected after an oral administration of pilocarpine (PIL). Under those conditions, PIL provoked a massive salivary secretion both in normal control mice and during the whole course of the IPR-induced gland enlargement. Striking changes in the polypeptide composition of saliva obtained from chronically IPR-stimulated animals were observed. Those changes consisted basically in the appearance and progressive increase in concentration of parotid polypeptides C-G and in the progressive diminution in concentration of a couple of normal salivary polypeptides (polypeptides A-B). The appearance of new polypeptides in saliva could be established unequivocally within the 24 h following the trophic adrenergic stimulation. On the other hand, salivary polypeptides induced in response to a single administration of IPR could be demonstrated as late as 7-9 days after the stimulation. Accordingly, detection of parotid polypeptides C-G in PIL-produced saliva obtained from IPR-stimulated mice has proved to be a highly advantageous method to evaluate salivary gland hypertrophy both at very early stages after the trophic stimulation and late after the occurrence of the trophic episode.
authors.affiliation: Unit of Cellular and Molecular Biology, Institute for Biomedical Sciences, Faculty of Medicine, University of Chile, PO Box 70061, Av. Independencia 1027, 7, Santiago, Chile. rlopez@machi.med.uchile.cl
authors.forename: Remigio
authors.initials: R
authors.lastname: López Solís
authors.forename: Margarita
authors.initials: M
authors.lastname: Puente Díaz
authors.forename: Irene
authors.initials: I
authors.lastname: Morales Bozo
authors.forename: Ulrike Kemmerling
authors.initials: UK
authors.lastname: Weis
authors.forename: Francisco
authors.initials: F
authors.lastname: Díaz
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002455:Cell Division; D006984:Hypertrophy; D007545:Isoproterenol / Q000008:administration & dosage; D008297:Male; D051379:Mice; D010306:Parotid Gland / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D010455:Peptides / Q000032:analysis*; D010862:Pilocarpine / Q000008:administration & dosage; D012463:Saliva / Q000737:chemistry* / Q000378:metabolism; D013997:Time Factors
chemical_list: D015415:Biomarkers; D010455:Peptides; D010862:Pilocarpine; D007545:Isoproterenol