title: [High-performance liquid chromatographic assay for plasma total homocysteine].
abstract: OBJECTIVE
  To establish a high-performance liquid chromatography (HPLC) assay with an internal standard for determining plasma total homocysteine.
  
  
  METHODS
  The Hcy-mixed disulfides or protein bound Hcy in the plasma were reduced with TCEP. Subsequently, the thiol-compounds in the samples were derivatized with a thiol-specific fluorogenic reagent, SBD-F. The derivate was then separated and detected by HPLC with fluorometric detection.
  
  
  RESULTS
  The detection limit was 0.625 mumol.L-1; the rate of recovery was 98.87%; the intra-assay coefficient of variation (CV) was less than 5.5%; and the inter-assay CV was less than 9.0%.
  
  
  CONCLUSIONS
  The method presented in this paper is simple, sensitive, precise, and stable.
authors.affiliation: Molecular Hematology Laboratory, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Chong-wen
authors.initials: CW
authors.lastname: Dai
authors.forename: Guang-sen
authors.initials: GS
authors.lastname: Zhang
authors.forename: Xian-ping
authors.initials: XP
authors.lastname: Wu
mesh_terms: D000328:Adult; D000368:Aged; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D015590:Fluorophotometry / Q000379:methods; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity
chemical_list: D006710:Homocysteine