The heat shock-induced cell cycle arrest is attenuated by weak electromagnetic fields.
Stress-induced effects in human acute leukaemia cells (HL-60) were studied by flow cytometry using the fluorescent dye carboxyfluorescein succinimidyl ester which allows the analysis of several successive cell generations for up to 10 days. Asynchronously cycling cells subjected to heat shock (30 min at 41 degrees C) responded in two distinct ways: while one fraction of the cell population (about 15%) re-entered the cell cycle after a short delay, other cells became arrested at different phases of the cell cycle and remained arrested for up to several days and finally underwent apoptosis. Weak electromagnetic fields (60 micro T, 50 Hz) alleviated the heat-induced block and the fraction of arrested cells was significantly smaller.
Tokalov
Sergey V
SV
Institut für Zoologie, Technische Universität Dresden, D-01062 Dresden, Germany.
Gutzeit
Herwig O
HO
D002453:Cell Cycle; D002455:Cell Division; D004574:Electromagnetic Fields; D005434:Flow Cytometry; D005452:Fluoresceins; D005456:Fluorescent Dyes; D018922:HL-60 Cells; D018869:Heat-Shock Response; D006801:Humans; D007700:Kinetics; D013388:Succinimides
C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D005452:Fluoresceins; D005456:Fluorescent Dyes; D013388:Succinimides