Metabolism of [1-13C] glucose in extracts and in immobilized rat glioma C6 cell cultures: effects of hypoxia.
The metabolism of [1-13C] glucose was followed in C6 rat glioma cells immobilized on a gel thread and in perchloric extracts of the same cells in culture. The results showed that the main metabolite of [1-13C] glucose is [3-13C] lactate. The effects of hypoxia were followed in the perchloric acid extracts of C6 cells. In normoxic conditions, the main metabolites produced by the cells were [3-'3C] lactate, [3-13C] alanine, [2-13C], [3-13C] and [4-13C] glutamate. Lactate newly synthesized from glucose appeared to be exported in the perfusion medium when living cells were immobilized in gel threads made of extracellular matrix. After 5 h of hypoxia, the lactate labelling measured in PCA cell extracts was increased that of glutamate decreased and the appearance of a spectral line at 66.01 ppm, identified as [1-13C] glycerol-3-phosphate, was observed. The data suggest that the synthesis of glycerol-3-phosphate in these cells might represent a sign of hypoxia.
Perrin
A
A
Unite de Biophysique Cellulaire et Moléculaire, CRSSA, BP 87, 38702 La Tronche, France.
Duborjal
H
H
Roudier
E
E
Massarelli
R
R
D000818:Animals; D002247:Carbon Isotopes; D002457:Cell Extracts / Q000737:chemistry; D015687:Cell Hypoxia / Q000502:physiology*; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D005947:Glucose / Q000378:metabolism*; D019344:Lactic Acid / Q000096:biosynthesis; D051381:Rats; D014407:Tumor Cells, Cultured
D002247:Carbon Isotopes; D002457:Cell Extracts; D019344:Lactic Acid; D005947:Glucose