title: [Transfusion of activated immune cells induced fluctuations of HCV titer without mutations in hepatitis C virus core and E2 region CTL epitopes].
abstract: OBJECTIVE
  To investigate the variations of HCV core and E2 region epitopes during transfusion of activated immune cells.
  
  
  METHODS
  Four patients receiving transfusion of activated immune cells were under continuously observation. HCV titers were measured by quantitive PCR. HCV core and E2 regions were cloned and sequences were analyzed by computer software.
  
  
  RESULTS
  During the follow-up the serum ALT levels and the HCV virus titers fluctuated greatly in each of these persons. After transfusion, no significant variations were observed in HCV core and E2 coding regions.
  
  
  CONCLUSIONS
  The alteration of host immune attacks could induce the fluctuations of HCV load without any mutations in the currently observed coding genes of the epitopes.
authors.affiliation: Institute of Hepatology, People's Hospital, Beijing University, Beijing 100044, China.
authors.forename: Qixin
authors.initials: Q
authors.lastname: Wang
authors.forename: Hongsong
authors.initials: H
authors.lastname: Chen
authors.forename: Xu
authors.initials: X
authors.lastname: Cong
authors.forename: Ran
authors.initials: R
authors.lastname: Fei
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.forename: Lai
authors.initials: L
authors.lastname: Wei
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D000410:Alanine Transaminase / Q000097:blood; D000939:Epitopes / Q000235:genetics*; D005260:Female; D014644:Genetic Variation; D016174:Hepacivirus / Q000235:genetics; D019698:Hepatitis C, Chronic / Q000097:blood / Q000628:therapy* / Q000821:virology*; D006801:Humans; D007167:Immunotherapy; D008297:Male; D008875:Middle Aged; D014758:Viral Core Proteins / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000235:genetics*; D019562:Viral Load
chemical_list: D000939:Epitopes; D014758:Viral Core Proteins; D014759:Viral Envelope Proteins; C069130:nucleocapsid protein, Hepatitis C virus; C091015:glycoprotein E2, Hepatitis C virus; D000410:Alanine Transaminase