title: Transcriptional gene expression profiles of oesophageal adenocarcinoma and normal oesophageal tissues.
abstract: The molecular events associated with the development of adenocarcinoma of the oesophagus are not well understood. Gene expression associated with oesophageal adenocarcinoma was investigated using a cDNA array containing 1,176 human cancer-associated genes. Approximately 59% of the genes were expressed at detectable levels with 15 genes (1.3%) exhibiting differential (> 2.5-fold) expression in either normal oesophagus or adenocarcinoma tissue. Nine genes were up-regulated in oesophageal adenocarcinoma tissue (matrix metalloproteinase 11 (MMP11), ornithine decarboxylase (ODC), cytokeratins 8 and 18, integrin alpha 3 (ITGA3), integrin alpha 6 (ITGA6), BIGH3 (transforming growth factor beta-induced), beta-catenin and CDC25B (M-phase inducer phosphatase 2)). Six genes were down-regulated in adenocarcinoma tissue (cytokeratin 4, plasminogen activator inhibitor 2 (PAI-2), interleukin 1 receptor antagonist (IRAP), cytokeratin 13/15/17, MAD and retinoic acid receptor gamma 1 (RARG)). Many of these differentially expressed genes influence cell-cell adhesion, cell-extracellular matrix and composition, transcriptional activation and cell cycle progression and are likely to contribute to development of oesophageal adenocarcinoma.
authors.affiliation: Department of Microbiology, University College Cork, Cork, Ireland.
authors.forename: R N
authors.initials: RN
authors.lastname: Hourihan
authors.forename: G C
authors.initials: GC
authors.lastname: O'Sullivan
authors.forename: J G
authors.initials: JG
authors.lastname: Morgan
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000235:genetics; D003598:Cytoskeletal Proteins / Q000096:biosynthesis / Q000235:genetics; D015342:DNA Probes; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000378:metabolism; D004947:Esophagus / Q000378:metabolism / Q000473:pathology; D016326:Extracellular Matrix Proteins; D020869:Gene Expression Profiling; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein; D007381:Intermediate Filament Proteins / Q000096:biosynthesis / Q000235:genetics; D005810:Multigene Family; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012795:Sialoglycoproteins / Q000096:biosynthesis / Q000235:genetics; D015534:Trans-Activators / Q000096:biosynthesis / Q000235:genetics; D014158:Transcription, Genetic; D016212:Transforming Growth Factor beta; D051176:beta Catenin; D000099222:betaIG-H3 Protein
chemical_list: D015815:Cell Adhesion Molecules; D003598:Cytoskeletal Proteins; D015342:DNA Probes; D016326:Extracellular Matrix Proteins; D053590:Interleukin 1 Receptor Antagonist Protein; D007381:Intermediate Filament Proteins; D009363:Neoplasm Proteins; D012795:Sialoglycoproteins; D015534:Trans-Activators; D016212:Transforming Growth Factor beta; D051176:beta Catenin; D000099222:betaIG-H3 Protein; C495270:CTNNB1 protein, human; C507763:IL1RN protein, human