Transmission confocal laser scanning microscopy with a virtual pinhole based on nonlinear detection.
We present a transmission-mode confocal laser scanning microscope system based on the use of second-harmonic generation (SHG) for signal detection. Our method exploits the quadratic intensity dependence of SHG to preferentially reveal unscattered signal light and reject out-of-focus scattered background. The SHG crystal acts as a virtual pinhole that remains self-aligned without the need for descanning.
Yang
C
C
Laboratoire de Neurophysiologie et Nouvelles Microscopies, Ecole Supérieure de Physique et Chimie Industrielles, Institut National de la Santé et de la Recherche Médicale EPI-0002, Paris, France.
Mertz
J
J
D018613:Microscopy, Confocal; D008962:Models, Theoretical