Capillary electrophoresis for simultaneous determination of emodin, chrysophanol, and their 8-beta-D-glucosides.
The simultaneous separation and determination of the major anthraquinones, emodin, chrysophanol, and their glucosides, of Rumex japonicus HOUTT., and emodin and emodin glucoside, of Cassia tora L., Rhamnus purshiana DC., Polygonum multiflorum THUNB., and P. cuspidatum SIEB. et ZUCC., were achieved by cyclodextrin modified capillary zone electrophoresis. The running electrolyte used in this method was 0.005 M alpha-cyclodextrin in 0.03 M borate buffer (pH 10.5) containing 10% acetonitrile, with an applied voltage of 20 kV.
Koyama
Junko
J
Kobe Pharmaceutical University, Higashinada-ku, Japan. j-koyama@kobepharma-u.ac.jp
Morita
Izumi
I
Kawanishi
Kazuko
K
Tagahara
Kiyoshi
K
Kobayashi
Norihiro
N
D000880:Anthraquinones / Q000032:analysis* / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods; D004642:Emodin / Q000032:analysis* / Q000737:chemistry; D005960:Glucosides / Q000032:analysis* / Q000737:chemistry; D024301:Plant Bark; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry; D018517:Plant Roots
D000880:Anthraquinones; D005960:Glucosides; D010936:Plant Extracts; D004642:Emodin; C027113:chrysophanic acid