[Preliminary study on the mechanism of connexin 43 gene transfection in the control of glioma cell proliferation].
OBJECTIVE
To study the mechanism involved in the control of glioma cell proliferation with transfection of connexin (Cx) 43 gene.


METHODS
C6 rat glioma and TJ905 human glioblastoma cell lines without Cx43 gene expression were transfected with Cx43cDNA mediated by lipofectamine. Northern blot, in situ hybridization and immunohistochemical technology were used to detect the expression of Cx43mRNA and its protein with MTT assay and silver colloid stain for the detection of cell proliferation, TUNEL method for determination of cell apoptosis, scrape loading dye transfer (SLDT) for GJIC, Western blot and immunohistochemical technology for bFGF, PDGF, EGFR, IGF-I and IGFBP3 expression.


RESULTS
Cx 43 gene transfected glioma cells showed decreased proliferation, restored GJIC and decreased bFGF, PDGF, IGFBP3, except EGFR expression and cell apoptosis which showed no change.


CONCLUSIONS
The mechanism of Cx 43 gene inhibiting gliomas cell proliferation is the restoration of GJIC and decreased autocrine growth factors.
Xia
Zhi-bai
ZB
Department of Neurosurgery, General Hospital, Tianjin Medical University, Tianjin 300052, China.
Pu
Pei-yu
PY
Huang
Qiang
Q
You
Yong-ping
YP
Wang
Guang-xiu
GX
Wang
Chun-yan
CY
D000818:Animals; D017209:Apoptosis; D002455:Cell Division / Q000502:physiology; D018031:Connexin 43 / Q000235:genetics / Q000502:physiology*; D018076:DNA, Complementary / Q000235:genetics; D005910:Glioma / Q000473:pathology*; D051381:Rats; D014162:Transfection; D014407:Tumor Cells, Cultured
D018031:Connexin 43; D018076:DNA, Complementary