[Expression of multidrug resistance P-170 and MRP and their correlation with clinical drug resistance in acute leukemia].
OBJECTIVE
To investigate the relevance between the expression of P-170 and MRP and clinical drug resistance in acute leukemia.


METHODS
The expression of P-170 and MRP in mononuclear cells of bone marrows was analyzed by the immunohistochemical technique in 72 acute leukemia patients.


RESULTS
The expression of P-170 was positive in 46 and negative in 26 of the 72 post-chemotherapy acute leukemia patients. The therapeutic effect of the P-170 positive expression patients was significantly poorer than that of the negative expression patients (P < 0.01). The expression of MRP was positive in 39 and negative in 33 of the 72 post-chemotherapy acute leukemia patients. The therapeutic effect of the MRP positive expression patients was significantly poorer than that in the negative expression patients (P < 0.01). The expression of P-170 and MRP had a significant concordance (Kappa = 0.427, P < 0.01). The sensitivity of P-170 and MRP which were analyzed simultaneously was 97.5%, which was higher than that of P-170 (90%) or MRP (77.5%) analyzed respectively in drug resistance patients.


CONCLUSIONS
The expression of P-170 and/or MRP was significantly related with drug resistance in clinical chemotherapy. The therapeutic effect was significantly poorer in P-170 and/or MRP positive expression patients than that in negative expression patients. These data suggest that P-170 and MRP analyzed simultaneously can improve the value of diagnosis and prognosis in patients with drug resistance leukemia.
Lin
Xiu-mei
XM
Department of Hematology, Xiangya Hospital, Central South University, Changsha 410008, China.
Xie
Zhao-xia
ZX
Zhu
Yan
Y
D018435:ATP Binding Cassette Transporter, Subfamily B; D000293:Adolescent; D000328:Adult; D018432:Drug Resistance, Multiple; D005260:Female; D006023:Glycoproteins / Q000096:biosynthesis*; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000096:biosynthesis*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics / Q000378:metabolism*; D011379:Prognosis
D018435:ATP Binding Cassette Transporter, Subfamily B; D006023:Glycoproteins; D027425:ATP-Binding Cassette, Sub-Family C Proteins; C513055:ABCB1 protein, human