Telomerase activity in condyloma acuminatum tissue with different HPV types.
The telomerase activity in condyloma acuminatum (CA) tissue with human papillomavirus (HPV) types of 6/11 and 16/18 was detected to investigate the function of telomerase in the occurrence, development and carcinogenesis of genital CA. Forty-two biopsies from patients with genital CA and 30 control tissue samples were tested for telomerase activity, HPV presence and types. The telomerase activity was determined by modified telomerase repeat amplification protocol (TRAP) assay and HPV typing by polymerase chain reaction (PCR) with typing-specific primers. Results showed that HPV-DNA was negative and the expression rate of telomerase was 16.7% in all normal skin samples. All CA samples were positive for HPV (6/11 type was found in 32 cases, 16/18 in 3 and mixed type in 7). Telomerase activity was detectable in all CA patients. The telomerase activity in CA of 16/18 type was apparently higher than in CA of 6/11 type. It was concluded that the hyperplasia in CA might be increased as a result of HPV infection, suggesting that the activation of telomerase by HPV, especially by 16/18 type may play a role in the etiology and carcinogenesis of genital CA.
Tu
Yating
Y
Department of Dermatology, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022.
Chen
Shanjuan
S
Fan
Chao
C
Lin
Nengxing
N
Liu
Houjun
H
Liu
Zhixiang
Z
D000328:Adult; D000368:Aged; D003218:Condylomata Acuminata / Q000201:enzymology* / Q000821:virology*; D005260:Female; D005831:Genital Diseases, Female / Q000821:virology; D005832:Genital Diseases, Male / Q000821:virology; D006801:Humans; D008297:Male; D008875:Middle Aged; D027383:Papillomaviridae / Q000145:classification*; D030361:Papillomavirus Infections / Q000201:enzymology / Q000821:virology; D019098:Telomerase / Q000378:metabolism*; D014412:Tumor Virus Infections / Q000201:enzymology / Q000821:virology
D019098:Telomerase