[Expression of full-length hepatitis E virus structural gene using baculovirus expression system].
OBJECTIVE
To obtain recombinant baculovirus containing full-length structural gene of HEV and to express HEV structural protein.


METHODS
Full-length structural gene of HEV (5147-7126 nt) was inserted into baculovirus expression vector pAcUW51. The recombinant plasmid and Baculo Gold DNA were co-transfected into insect cell line Sf9. Single plaque was picked and amplified and expression of HEV structural protein was tested by SDS-PAGE, Western blot and immunofluorescence methods.


RESULTS
SDS-PAGE analysis showed HEV structural protein was highly expressed; Western blot and immunofluorescence assay showed that the expression product could specifically react with HEV positive sera, confirming the protein possessing HEV specific antigenicity.


CONCLUSIONS
HEV structural protein was successfully expressed using baculovirus expression system.
Zhang
Mingcheng
M
Hepatitis Branch, Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China.
Yi
Yoo
Y
Liu
Chongbai
C
Bi
Shengli
S
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D002478:Cells, Cultured; D015870:Gene Expression; D005796:Genes / Q000235:genetics*; D005822:Genetic Vectors; D016752:Hepatitis E virus / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D015678:Viral Structural Proteins / Q000096:biosynthesis* / Q000235:genetics
D011994:Recombinant Proteins; D015678:Viral Structural Proteins