title: Whole CagA gene amplification of Helicobacter pylori and its fingerprinting by restriction fragment length polymorphism.
abstract: To set up a method of amplification for the whole CagA gene of Helicobacter pylori and its fingerprinting by restriction fragment length polymorphism (RFLP), nested PCR was employed in combination with TD-PCR to amplify the gene and EcoRI and Hind III were used to generate the RFLP fingerprinting. Target DNA fragments from 13 of 20 samples were successfully amplified and the relevant RFLP fingerprintings were obtained. It is concluded that the method can be used to amplify the whole CagA gene and CagA gene has apparent diversity of RFLP profile.
authors.affiliation: Department of Pathogenic Organisms, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
authors.forename: Siying
authors.initials: S
authors.lastname: Ye
authors.forename: Jienan
authors.initials: J
authors.lastname: Ao
authors.forename: Ying
authors.initials: Y
authors.lastname: Peng
authors.forename: Haifeng
authors.initials: H
authors.lastname: Yue
authors.forename: Fang
authors.initials: F
authors.lastname: Liao
authors.forename: Guoping
authors.initials: G
authors.lastname: Hu
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
authors.forename: Zhengmao
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D016172:DNA Fingerprinting / Q000379:methods; D005784:Gene Amplification / Q000235:genetics*; D016480:Helicobacter pylori / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C080866:cagA protein, Helicobacter pylori