Effects of the tyrosine kinase inhibitor tyrphostin AG 556 on acute necrotising pancreatitis in rats.
OBJECTIVE
To investigate the effects of the tyrosine kinase inhibitor tyrphostin AG 556 on the course of acute necrotising pancreatitis in rats.


METHODS
Laboratory study.


METHODS
Medical school, Turkey.


METHODS
72 Sprague Dawley rats, 12 in the sham operated (control) group and 20 in each of the three others.


METHODS
Cardiorespiratory measurements, mortality, effect on the activities of various enzymes in serum and tissue of pancreas and lung, and the histological picture.


RESULTS
The four study groups were sham + Ringer's lactate, acute necrotising pancreatitis with Ringer's lactate, tryphostin AG 556, and dimethylsulfoxide (DMSO). There were 12 animals in the first group and 20 in each of the other groups. The induction of pancreatitis increased mortality from 0/12 in the control to 6/20 (30%), 7/20 (35%), 8/20 (40%) in the three experimental groups, respectively. Heart rate, packed cell volume (PCV), serum activities of amylase and alanine aspartate transferase, tissue activity of myeloperoxidase (MPO) and malondialdehyde (MDA) in the pancreas and lung, serum concentrations of urea and calcium, volume of ascites, degree of pancreatic damage, blood pressure, and urine production did no differ between the pancreatitis groups.


CONCLUSIONS
Treatment with the tyrphostin kinase inhibitor did not improve the course of acute pancreatitis or reduce the extent of acinar cell injury and is therefore unlikely to be of benefit in patients with pancreatitis.
Alhan
Etem
E
Department of Surgery and Biochemistry, Karadeniz Technical University, Farabi Hospital, Trabzon, Turkey. alhan@meds.ktu.edu.tr
Cicek
Ramazan
R
Erçin
Cengiz
C
Orem
Asim
A
Vanizor
Birgül
B
Cinel
Akif
A
D000410:Alanine Transaminase / Q000097:blood; D000681:Amylases / Q000097:blood; D000818:Animals; D004121:Dimethyl Sulfoxide / Q000627:therapeutic use; D004791:Enzyme Inhibitors / Q000627:therapeutic use*; D016166:Free Radical Scavengers / Q000627:therapeutic use; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D023421:Models, Animal; D009102:Multiple Organ Failure / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D010179:Pancreas / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D019283:Pancreatitis, Acute Necrotizing / Q000150:complications / Q000188:drug therapy*; D009195:Peroxidase / Q000032:analysis; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020032:Tyrphostins / Q000627:therapeutic use*
D004791:Enzyme Inhibitors; D016166:Free Radical Scavengers; D020032:Tyrphostins; D008315:Malondialdehyde; D009195:Peroxidase; D000410:Alanine Transaminase; D011505:Protein-Tyrosine Kinases; D000681:Amylases; D004121:Dimethyl Sulfoxide