Nucleotide sequences of the coat protein gene of potyviruses infecting Ornithogalum thyrsoides.
Species of three viral genera infecting Ornithogalum thyrsoides plants showing mosaic symptoms were identified using RT-PCR and degenerate universal primers for each viral genus. The DNA fragments obtained encoded the coat protein (CP) gene and were sequenced. The plants were found to be infected with one or other of three potyvirus species, one of them was Ornithogalum mosaic virus (OrMV). The other two viruses were previously unrecorded and were named Ornithogalum virus 2 (OV-2) and 3 (OV-3). Direct comparison and phylogenetic analysis with published OrMV isolates revealed that the CP of the three OrMV-like clones were more similar to Pterostylis virus Y (PtVY) than to OrMV. No carlavirus or potexvirus was isolated.
Fuji
S
S
Faculty of Bioresource Sciences, Akita Prefectural University, Akita, Japan. sfuji@akita-pu.ac.jp
Inoue
M
M
Yamamoto
H
H
Furuya
H
H
Naito
H
H
Matsumoto
T
T
D001483:Base Sequence; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics*; D017931:DNA Primers; D018076:DNA, Complementary / Q000096:biosynthesis / Q000737:chemistry; D005814:Genes, Viral; D008969:Molecular Sequence Data; D031442:Ornithogalum / Q000821:virology*; D010802:Phylogeny; D017800:Potyvirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
D036022:Capsid Proteins; D017931:DNA Primers; D018076:DNA, Complementary