title: Immunohistochemical and ultrastructural study on effect of fibroblast growth factor on transformation of fibroblasts to regenerated mesothelial cells.
abstract: To elucidate the effect of fibroblast growth factor on the phenotypical conversion of fibroblasts to mesothelial cells, both immunohistochemical and ultrastructural examinations were carried out on cultured spheroids that were composed of fibroblasts obtained from the parietal pleura of rats with and without addition of antifibroblast growth factor receptor antibody. In the present study, antifibroblast growth factor receptor antibody was employed to block the effect of the autocrine component of fibroblast growth factor in the culture medium. Phenotypical conversion from fibroblast to mesothelial cells was clearly blocked in the experimental group, to which culture medium had been added with antifibroblast growth factor receptor antibody, whereas the control group, cultured without addition of antifibroblast growth factor receptor antibody, showed phenotypical conversion of fibroblasts that was confirmed by the development of macula adherens, microvilli, and positive expression of cytokeratin. These results indicate the possibility that fibroblast growth factor plays a key role in the process of phenotypic conversion of fibroblasts to regenerated mesothelial cells.
authors.affiliation: Division of Electronmicroscopy, Ohashi Hospital, Toho University School of Medicine, 2-17-6 Ohashi, Meguro-ku, Tokyo 153-8515, Japan. amari@oha.toho-u.ac.jp
authors.forename: Masao
authors.initials: M
authors.lastname: Amari
authors.forename: Katsuji
authors.initials: K
authors.lastname: Taguchi
authors.forename: Minoru
authors.initials: M
authors.lastname: Iwahara
authors.forename: K
authors.initials: K
authors.lastname: Shibuya
authors.forename: Shiro
authors.initials: S
authors.lastname: Naoe
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D004847:Epithelial Cells; D005346:Fibroblast Growth Factors / Q000302:isolation & purification / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005346:Fibroblast Growth Factors