Human osteoclast maturation from bone marrow cells co-cultured with osteoblast from ethmoid sinus.
In chronic sinusitis, although the pathogenesis in the sinus mucosa has been widely investigated, the pathogenesis in the underlying bone remains poorly understood. As a first step in investigating the pathogenesis in sinus bone, sinus-bone models should be constructed by co-culturing of osteoblasts and osteoclasts in a sinus. However, human osteoclast cell lines derived from a sinus have not been established. Based on previous methods of another osteoclast culture, we hypothesized that human osteoclasts in sinuses could be established in vitro by co-culture with human osteoblasts in the sinus. The aim of this study was to differentiate human bone marrow cells into osteoclasts in a sinus, which is identified by the formation of absorption lacuna and positive cytochemical staining for tartrate-resistant acid phosphatase (TRAP). Differentiation was induced in the co-culture system by treatment with medium containing 1.25.(OH)2D3, G-CSF and dexamethasone. Thirty days after culturing, human bone marrow cells were differentiated to a hypertrophic state, as evidenced by their sizes and the demonstration of osteoclast-specific abilities. Our results indicate that sinus-osteoclasts can be cultured from human bone marrow cells, and that osteoclasts in a sinus may be a useful model for investigating sinus-bone remodeling, especially in terms of bone resorption, in the sinus system.
Ishino
Takashi
T
Department of Otorhinolaryngology/Head & Neck Surgery, Hiroshima University School of Medicine, Hiroshima city, Hiroshima, Japan. takashiishino@hotmail.com
Yajin
Koji
K
Takeno
Sachio
S
Furukido
Kyousuke
K
Takumida
Masaya
M
Hirakawa
Katsuhiro
K
D000135:Acid Phosphatase; D001854:Bone Marrow Cells / Q000166:cytology*; D016723:Bone Remodeling; D001862:Bone Resorption / Q000503:physiopathology; D002454:Cell Differentiation; D018920:Coculture Techniques; D006801:Humans; D007527:Isoenzymes; D010006:Osteoblasts / Q000166:cytology*; D010010:Osteoclasts / Q000502:physiology*; D000071681:Tartrate-Resistant Acid Phosphatase
D007527:Isoenzymes; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase