Influence of anti-CD49f and anti-CD29 monoclonal antibodies on mitotic activity of epithelial cells (HaCaT) and gingival fibroblasts in vitro.
A major complication in the treatment of periodontitis marginalis is the reepithelization of periodontal defects inhibiting collagen fiber attachment and periodontal regeneration. In this study we investigated the possibility of a molecular blockade of epithelial mitosis in vitro. Monoclonal antibodies against the VLA-6 laminin receptor subunit alpha 6 interrupted interactions between epithelial cells (HaCaT cells) and their extracellular matrix and thus resulted in reduction of proliferation rates by more than 50%. The same effect was observed with anti beta 1-antibodies. In contrast, collagen-producing and -secreting gingival fibroblasts, which play an important role in periodontal regeneration, remained unaffected by the applied anti alpha 6 antibodies. Correspondingly, these cells were found to lack VLA-6 laminin receptors. Selective molecular inhibition of epithelial proliferation and apical migration by monoclonal anti alpha 6 antibody application may provide an adjuvant periodontitis therapy resulting in an enhanced periodontal regeneration.
Becker
Christoph
C
Department for Operative Dentistry, Periodontology and Preventive Dentistry, Medical Faculty, RWTH Aachen, Pauwelsstrasse 30, D-52074 Aachen, Germany. cbecker@ukaachen.de
Buttler
Patricia
P
Gräber
Hans Georg
HG
D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D002448:Cell Adhesion / Q000502:physiology; D002450:Cell Communication / Q000502:physiology; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology; D003094:Collagen / Q000378:metabolism; D004846:Epithelial Attachment / Q000166:cytology*; D005109:Extracellular Matrix / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D005881:Gingiva / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D039503:Integrin alpha6 / Q000502:physiology*; D019012:Integrin beta1 / Q000502:physiology*; D008938:Mitosis / Q000502:physiology*
D000911:Antibodies, Monoclonal; D039503:Integrin alpha6; D019012:Integrin beta1; D003094:Collagen