Up-regulation of caveolin expression by cytotoxic agents in drug-sensitive cancer cells.
Caveolin 1 expression is down-regulated in various cancer cell lines. Interestingly, in several drug-resistant cancer cells, a strong induction of caveolin 1 expression has been reported, suggesting a role for caveolin 1 in the acquisition and/or the maintenance of the multidrug-resistance phenotype. Here, we show, in drug-sensitive lung cancer cells (A549, Calu-6 or NCI-H69), that exposure to cytotoxic drugs (taxol, doxorubicin or etoposide) is sufficient to strongly up-regulate caveolin 1 and 2 protein levels. This up-regulation is sustained even 1 week after drug removal. Our results suggest that caveolin up-regulation is an early cellular response to a cytotoxic stress taking place before drug resistance.
Bélanger
Martin M
MM
aInstitut Universitaire de Cardiologie et de Pneumologie de l'Université Laval, Unité de Recherche en Pneumologie, Centre de Lecherche Hôpital Laval, Sainte-Foy (Quebec) G1 V 4G5, Canada.
Roussel
Elise
E
Couet
Jacques
J
D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D015152:Blotting, Northern; D051242:Caveolin 1; D051243:Caveolin 2; D022461:Caveolins / Q000096:biosynthesis* / Q000235:genetics; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm; D005047:Etoposide / Q000494:pharmacology; D006801:Humans; D015151:Immunoblotting; D017239:Paclitaxel / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis; D015854:Up-Regulation
D000970:Antineoplastic Agents; C495540:CAV1 protein, human; D051242:Caveolin 1; D051243:Caveolin 2; D022461:Caveolins; D012333:RNA, Messenger; D005047:Etoposide; D004317:Doxorubicin; D017239:Paclitaxel