Influence of green tea polyphenol in rats with arginine-induced renal failure.
To determine whether green tea polyphenol ameliorates the pathological conditions induced by excessive dietary arginine, green tea polyphenol was administered to rats at a daily dose of 50 or 100 mg/kg body weight for 30 days with a 2% w/w arginine diet. In arginine-fed control rats, urinary and/or serum levels of guanidino compounds, nitric oxide (NO), urea, protein, and glucose increased significantly, while the renal activities of the oxygen species-scavenging enzymes superoxide dismutase (SOD) and catalase decreased, compared with casein-fed rats. However, rats given green tea polyphenol showed significant and dose-dependent decreases in serum levels of creatinine (Cr) and urea nitrogen and urinary excretion of Cr, and they exerted a slight reduction of nitrite plus nitrate, indicating that green tea polyphenol reduced the production of uremic toxins and NO. In addition, in arginine-fed rats the urinary urea, protein, and glucose level increases were reversed by the administration of green tea polyphenol. Moreover, in rats given green tea polyphenol the SOD and catalase activities suppressed by excessive arginine administration increased dose-dependently, implying the biological defense system was augmented as a result of free radical scavenging. These results suggest that green tea polyphenol would ameliorate renal failure induced by excessive dietary arginine by decreasing uremic toxin, and NO production and increasing radical-scavenging enzyme activity.
Yokozawa
Takako
T
Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan. yokozawa@ms.toyama-mpu.ac.jp
Cho
Eun Ju
EJ
Nakagawa
Takako
T
D000818:Animals; D001120:Arginine / Q000008:administration & dosage* / Q000633:toxicity*; D001806:Blood Urea Nitrogen; D002374:Catalase / Q000378:metabolism; D003404:Creatinine / Q000097:blood / Q000652:urine; D005419:Flavonoids; D005979:Glutathione Peroxidase / Q000378:metabolism; D006146:Guanidines / Q000097:blood / Q000652:urine; D007668:Kidney / Q000201:enzymology; D008297:Male; D009569:Nitric Oxide / Q000097:blood / Q000652:urine; D010636:Phenols / Q000008:administration & dosage*; D011108:Polymers / Q000008:administration & dosage*; D059808:Polyphenols; D051381:Rats; D017208:Rats, Wistar; D051437:Renal Insufficiency / Q000139:chemically induced* / Q000188:drug therapy* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013662:Tea / Q000737:chemistry*
D005419:Flavonoids; D006146:Guanidines; D010636:Phenols; D011108:Polymers; D059808:Polyphenols; D013662:Tea; D009569:Nitric Oxide; D001120:Arginine; D003404:Creatinine; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase