title: Modulation of Basophil Histamine Release and T Cell Reactivity by Chemically Modified Allergen.
abstract: Modification of a model allergen ovalbumin (OA) with succinylation (Suc-OA) led to inhibition of allergen histamine releasing activity as it was tested on basophils of OA-sensitive patients. A whole blood leukocyte histamine release was performed by glass fibre based histamine assay. IgE-binding activity of Suc-OA was significantly reduced as it was shown by RAST inhibition technique. Suc-OA stimulated OA-specific T cell hybrid 3DO-548 and ACP:LK35 to produce cytokine release at the same level as in the case of non-modified OA. Succinylation of OA was concluded to result in selective blockade of B cell and preservation of T cell epitopes of the allergen suggesting a new approach for allergen specific immunotherapy.
authors.affiliation: Institute of Immunology, Moscow, Russia.
authors.forename: I. S.
authors.initials: IS
authors.lastname: Gushchin
authors.forename: A. A.
authors.initials: AA
authors.lastname: Babakhin
authors.forename: S. M.
authors.initials: SM
authors.lastname: Andreev
authors.forename: A. W.
authors.initials: AW
authors.lastname: Wheeler
authors.forename: B.
authors.initials: B
authors.lastname: Stockinger
authors.forename: H.
authors.initials: H
authors.lastname: Nolte
authors.forename: L. M.
authors.initials: LM
authors.lastname: DuBuske