title: Mast-cell activation augments the late phase reaction in experimental immune-mediated blepharoconjunctivitis.
abstract: BACKGROUND
  How the early phase allergic reaction affects the late phase reaction remains unclear. We examined this issue with an experimental model of allergic conjunctivitis that permits the two reactions to be disconnected from each other.
  
  
  METHODS
  Experimental immune-mediated blepharoconjunctivitis (EC) was initiated in Brown Norway rats by transferring ovalbumin (OVA)-specific T cells and then challenging with OVA-containing eye drops. To induce early phase reaction, a mast-cell activator, C48/80, was challenged together with or without OVA. Rats were evaluated clinically and eyes were harvested for histologic examination and for evaluation of chemokine expression by reverse-transcriptase PCR.
  
  
  RESULTS
  The rats challenged with OVA alone developed the T-cell-mediated late phase reaction histologically, but not clinically, in the absence of early phase reaction. While rats challenged with C48/80 with or without OVA exhibited clinical signs of the early phase reaction, the clinical late phase reaction was observed only in the OVA+C48/80 group. Eosinophilic infiltration into the conjunctiva during the late phase reaction of the OVA+C48/80 group markedly exceeded that of rats challenged with either OVA or C48/80 alone. RANTES (regulated on activation, normal T-cell expressed and secreted), an eosinophil attractant, was expressed both in the OVA+C48/80 and OVA groups, while eotaxin was expressed at equivalent levels in all three groups.
  
  
  CONCLUSIONS
  The mast-cell-mediated early phase reaction potentiates the T-cell-mediated late phase reaction, and RANTES is involved in eosinophilic infiltration induced by antigen-specific T cells. Other molecules induced by allergen-specific T cells activated in an as yet unknown manner by the mast cells may be responsible for the infiltration of eosinophils.
authors.affiliation: Laboratory of Immunology, Department of Ophthalmology, Kochi Medical School, Kohasu, Oko-cho, 783-8505 Nankoku-city, Japan.
authors.forename: Akemi
authors.initials: A
authors.lastname: Ozaki
authors.forename: Atsuki
authors.initials: A
authors.lastname: Fukushima
authors.forename: Kazuyo
authors.initials: K
authors.lastname: Fukata
authors.forename: Hisayuki
authors.initials: H
authors.lastname: Ueno
mesh_terms: D000818:Animals; D001762:Blepharitis / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology; D002465:Cell Movement; D018946:Chemokine CCL5 / Q000378:metabolism; D003233:Conjunctivitis, Allergic / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D004802:Eosinophilia / Q000276:immunology; D004804:Eosinophils / Q000502:physiology; D005434:Flow Cytometry; D006968:Hypersensitivity, Delayed / Q000276:immunology* / Q000473:pathology; D008213:Lymphocyte Activation; D008297:Male; D008407:Mast Cells / Q000502:physiology*; D010047:Ovalbumin / Q000276:immunology; D051381:Rats; D011914:Rats, Inbred BN; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000276:immunology; D003189:p-Methoxy-N-methylphenethylamine
chemical_list: D018946:Chemokine CCL5; D003189:p-Methoxy-N-methylphenethylamine; D010047:Ovalbumin