[An experimental study on the role of indirect antigen presentation pathway on the immunogenetic of epidermal cells].
OBJECTIVE
To explore the role of indirect antigen presentation pathway on the immunogenecity of epidermal cells.


METHODS
Human epidermal cells (HEC), allogeneic human peripheral blood lymphocytes (PBL) and mononuclear cells (PBM, including monocytes) were isolated and cultured in vitro. HECs were transfected by human-originated CTLA4Ig-adenovirus vector. The CTLA4Ig expression was observed. Allogeneic PBLs or PBMs were added to the transfected and non-transfected HECs with simple cultured PBLs and PBMs as the control. The proliferation of PBL and PBM was determined by (3)H-TdR incooperation.


RESULTS
HECs could be successfully transfected by CTLA4Ig-adenovirus vector and expressed corresponding proteins. The non-transfected HECs could stimulate slight proliferation of allogeneic PBLs (P < 0.05) and stimulate remarkable proliferation of PBMs (including monocytes) (P < 0.05). The proliferation reaction of PBLs and PBMs decreased significantly (P < 0.05) after being stimulated by HEC which was modulated by CTLA4Ig genes.


CONCLUSIONS
Indirect antigen presentation pathway might play important roles in the HEC immunogenicity which could be evidently inhibited by CTLA4Ig.
Chen
Xi-Wei
XW
Institute of Burn Research, Southwestern Hospital, The Third Military Medical University, Institute of Organ Transplantation Research of Chongqing City. Chongqing, 400038. P.R. China.
Wu
Jun
J
Luo
Qi-Zhi
QZ
Luo
Gao-Xing
GX
Wang
Xi-Hua
XH
Yi
Shao-Xuan
SX
He
Wei-Feng
WF
Ma
Bing
B
Wang
Ru-Peng
RP
Tang
Jin
J
D000256:Adenoviridae / Q000235:genetics; D017951:Antigen Presentation / Q000276:immunology* / Q000502:physiology; D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000235:genetics / Q000276:immunology; D060908:CTLA-4 Antigen; D002455:Cell Division / Q000276:immunology; D002478:Cells, Cultured; D018920:Coculture Techniques; D000078404:Epidermal Cells; D004817:Epidermis / Q000276:immunology* / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology; D015398:Signal Transduction; D014162:Transfection
D015703:Antigens, CD; D000943:Antigens, Differentiation; D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human