[Hemocytes of the blowfly Calliphora vicina and their dynamics during larval development and metamorphosis].
On the basis of in vitro observation of live cells and examination of stained slides of larval and prepupal Calliphora vicina hemolymph, seven types of hemocytes have been detected: prohemocytes, stable and unstable hyaline cells, thrombocytoids, spindle cells, larval plasmatocytes, and plasmatocytes I-IV, a. The last representing sequential stages of one cell line differentiation. Prohemocytes are basic cells, from which other forms of hemocytes derive outside the hemopoietic tissue, i.e. in free hemolymph. At the last larval instar, three waves of hemopoiesis occur. Either wave tends to increase the general number of cells and to change the quality of hemocyte population. The first wave occurs at the close of larva feeding and is accompanied by increase in the number of hyaline hemocytes, thrombocytoids and larval plasmatocytes. The second wave of hemopoiesis occurs after the larva's crop emptying. In this period the main increase of hemocyte population occurs at the expense of prohemocytes and plasmatocytes I. The most significant (five-fold) explosion of the population of free hemocytes takes place at the onset of pupariation and correlates with the rise of ecdysone titer. At the first stage of this peak, the amount of plasmatocytes I sharply increases. Further on these are rapidly differentiated into plasmatocytes II and III. After the puparium formation, hemocytes are reduced in number. Plasmatocytes III phagocytose fragments of destroyed larval tissues, pass to the stage of plasmatocytes IV (macrophages), and partially settle on tissues.
Kind
T V
TV
Laboratory of Entomology, Biological Institute of St. Petersburg State University. Tatiana.Kind@paloma.spbu.ru
D000818:Animals; D001772:Blood Cell Count; D002454:Cell Differentiation / Q000502:physiology; D004175:Diptera / Q000502:physiology*; D006434:Hemocytes / Q000166:cytology / Q000502:physiology*; D006458:Hemolymph / Q000166:cytology / Q000502:physiology; D007814:Larva / Q000166:cytology / Q000502:physiology; D008675:Metamorphosis, Biological / Q000502:physiology*