title: Nosocomial infectious potency of imipenem-resistant Pseudomonas aeruginosa isolated from obstetric and gynecologic infections.
abstract: Two hundred and eighty-two strains of Pseudomonas aeruginosa were isolated from patients at the Department of Obstetrics and Gynecology, Gifu University School of Medicine, between 1998 and 2000, and among them, 27 imipenem (IPM)-resistant strains of P. aeruginosa (27/282; 9.6%) were isolated from urine, exudate from surgical wounds, and ascites. Seven of the 27 IPM-resistant P. aeruginosa strains were positive (25.9%) for the bla (imp) gene determined by a polymerase chain reaction (PCR) method, and 4 of them were positive for the aac (6')-Ib gene. No strain of P. aeruginosa had both the bla (imp) and aac(6')-Ib genes. Pulsed-field gel electrophoresis (PFGE) patterns indicated the nosocomial spread of IPM-resistant P. aeruginosa. The presence of IPM-resistant P. aeruginosa carrying the bla (imp) gene might be implicated in possible spreading and outbreaks, and screening for bla (imp)-positive P. aeruginosa, therefore, might be necessary for protection against nosocomial infection.
authors.affiliation: Department of Obstetrics and Gynecology, Division of Organ Pathobiology, Gifu University School of Medicine, 40 Tsukasa-machi, Gifu 500-8705, Japan.
authors.forename: Xiang-Hua
authors.initials: XH
authors.lastname: Yin
authors.forename: Hiroshige
authors.initials: H
authors.lastname: Mikamo
authors.forename: Teruhiko
authors.initials: T
authors.lastname: Tamaya
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003428:Cross Infection / Q000382:microbiology / Q000517:prevention & control*; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D006801:Humans; D015378:Imipenem / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D009775:Obstetrics and Gynecology Department, Hospital; D016133:Polymerase Chain Reaction; D011552:Pseudomonas Infections / Q000382:microbiology / Q000517:prevention & control; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000201:enzymology; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D015378:Imipenem; D001618:beta-Lactamases