The effect of polyols on the reactivation of guanidium chloride-denatured arginine kinase from shrimp feneropenaeus chinensis muscle.
The influence of glycerol, sorbitol, glucose and sucrose, on the refolding and reactivation courses of guanidine-denatured arginine kinase was studied. Glycerol, sucrose, and sorbitol, but not glucose, could improve the reactivation of the denatured arginine kinase, although in all cases aggregation was inhibited. Size exclusion chromatography showed that misfolded products were still formed during polyol-assisted refolding. The chemical and physical characteristics of polyols might explain the various observations.
Yu
Zhenhang
Z
Molecular and Cellular Biology Graduate Program, and Department of Chemistry, Tulane University, New Orleans, Louisiana 70118, USA. yuzhenhang@tsinghua.org.cn
Li
Biao
B
D000818:Animals; D001122:Arginine Kinase / Q000037:antagonists & inhibitors / Q000187:drug effects* / Q000378:metabolism; D002850:Chromatography, Gel; D002942:Circular Dichroism; D033364:Decapoda / Q000201:enzymology*; D004793:Enzyme Reactivators / Q000494:pharmacology*; D019791:Guanidine / Q000378:metabolism; D009132:Muscles / Q000201:enzymology*; D011108:Polymers / Q000494:pharmacology*; D017510:Protein Folding; D013050:Spectrometry, Fluorescence; D013696:Temperature
D004793:Enzyme Reactivators; D011108:Polymers; C024617:polyol; D001122:Arginine Kinase; D019791:Guanidine