Rapid determination of total bromide in human serum using an energy-dispersive X-ray spectrometer.
Since the bromide preparations useful in the treatment of intractable infantile epilepsy show a tendency to accumulate in the body, they may cause chronic toxicosis. To prevent this, determination of the bromide ion concentration in the serum is essential. After establishing a simple and rapid technique using energy-dispersive X-ray spectroscopy for the analysis of the serum total bromide level, we applied this technique in a clinically diagnosed epilepsy patient. The standard curve for total bromide showed linearity (r=0.999) in the range of 10-2000 microg/ml, and the lowest detection limit was 5 microg/ml. The mean recovery rate of bromide added to reference serum to yield a concentration of 50 microg/ml was 93.5% (n=5, coefficient of variation=9.1%). Analysis took only 20 min. On analysis of the serum of a 10-year-old girl whose treatment was initiated with orally administered potassium bromide 1.0 g/kg, a good correlation was found between the total bromide level obtained with energy-dispersive X-ray spectroscopic analysis and the level of bromide ions determined by ion-exchange HPLC. The determination of serum total bromide by rapid energy-dispersive X-ray spectroscopic analysis is a useful method of monitoring to prevent bromide poisoning.
Tanaka
Hiroko
H
Department of Hospital Pharmacy, Niigata City General Hospital, Japan. hiror-ta.03@c7.mnx.ne.jp
Nakajima
Mariko
M
Fujisawa
Manami
M
Kasamaki
Masatoshi
M
Hori
Yasushi
Y
Yoshikawa
Hideto
H
Kitagawa
Shuji
S
D001965:Bromides / Q000097:blood* / Q000627:therapeutic use; D002648:Child; D005260:Female; D006801:Humans; D017680:Potassium Compounds / Q000097:blood* / Q000627:therapeutic use; D013052:Spectrometry, X-Ray Emission / Q000379:methods
D001965:Bromides; D017680:Potassium Compounds; C039004:potassium bromide