Droperidol attenuates contractile and phosphatidylinositol responses of rat trachea.
Droperidol attenuates airway smooth muscle contraction. However, the intracellular mechanisms involved in the droperidol-induced attenuation of airway smooth muscle contraction are not fully understood. We examined the effects of droperidol on contractile and phosphatidylinositol responses of the rat trachea. Droperidol dose dependently attenuated carbachol (CCh) induced tracheal ring contractions. The IC(50) of droperidol on CCh-induced tracheal ring contraction was 13 +/- 2 micromol/l. Droperidol attenuated CCh and aluminum fluoride stimulated inositol monophosphate accumulation. These results suggest that droperidol inhibits G protein coupled phospholipase C, resulting in attenuation of CCh-induced phosphatidylinositol response and subsequent attenuation of contractions of the rat trachea.
Shibata
Osamu
O
Department of Anesthesiology, Nagasaki University School of Medicine, Nagasaki, Japan. opshiba@net.nagasaki-u.ac.jp
Saito
Masataka
M
Makita
Tetsuji
T
Morooka
Hiroaki
H
Sumikawa
Koji
K
D000759:Adjuvants, Anesthesia / Q000494:pharmacology*; D000818:Animals; D000932:Antiemetics / Q000494:pharmacology*; D002217:Carbachol / Q000494:pharmacology; D018678:Cholinergic Agents / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004329:Droperidol / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology; D010716:Phosphatidylinositols / Q000096:biosynthesis*; D010744:Phosphoric Monoester Hydrolases / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D014132:Trachea / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology
D000759:Adjuvants, Anesthesia; D000932:Antiemetics; D018678:Cholinergic Agents; D010716:Phosphatidylinositols; D002217:Carbachol; D010744:Phosphoric Monoester Hydrolases; C021822:myo-inositol-1 (or 4)-monophosphatase; D004329:Droperidol