Study on the effects of FCu-IUD and FICu-IUD on matrix metalloproteinases in human uterine flushing and endometrium.
The activity of matrix metalloproteinases (MMPs) in the uterine flushing and endometrial tissue of normal adult women wearing FCu-IUD (fixed Cu-IUD) or FICu-IUD (indomethacin-releasing FCu-IUD) was observed by using zymography on SDS-PAGE containing gelatin. The results showed that the activity and kinds of MMPs in FCu-IUD group were increased significantly as compared with themselves before being inserted FCu-IUD. However, compared with the FCu-IUD group, the activity of some kinds of MMPs in the FICu-IUD group was decreased significantly. These data suggest that IUD can enhance the activity of MMPs in human endometrium, intermediated by prostaglandins, and MMPs may have relation to IUD-induced menorrhagia and indomethacin reduces IUD-induced menorrhagia by partly inhibiting MMPs synthesis.
Li
Wei
W
Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
Cao
Lumin
L
Chen
Zaojao
Z
Li
Wan
W
Du
Qingling
Q
Chen
Guiping
G
D000328:Adult; D004717:Endometrium / Q000201:enzymology*; D005260:Female; D006801:Humans; D007213:Indomethacin; D007435:Intrauterine Devices, Copper / Q000009:adverse effects*; D007436:Intrauterine Devices, Medicated / Q000009:adverse effects*; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D008875:Middle Aged; D014592:Uterine Hemorrhage / Q000209:etiology / Q000517:prevention & control
D020782:Matrix Metalloproteinases; D007213:Indomethacin