title: [Inhibition of Luciferase expression in mammalian cells by AAV vector plasmid mediated Luciferase shRNA].
abstract: OBJECTIVE
  Constructing a plasmid containing the shRNA of luciferase to suppress the expression of luciferase in BHK-21 cell.
  
  
  METHODS
  A 334 bp human U6 snRNA promoter was amplified from human genomic DNA by PCR and ligated to a 21 bp reverse repeated motif of luciferase target sequence with 9 bp spacer and AAV plasmid pSNAV. The recombinant pSNAV/U6/Luc plasmid cotransfected with pMAMneoLuc or transfected luciferase cell line to detect the effect of luciferase expression separately.
  
  
  RESULTS
  pSNAV/U6/luc suppresses the luciferase expression from pMAMneoLuc by 50% and luciferase cell line by 70%.
  
  
  CONCLUSIONS
  The results showed that the short hairpin RNA of luciferase can efficiently suppress its expression in BHK-21.
authors.affiliation: Institute of Virology,Chinese Academy of Preventive Medicine, Beijing 100052, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Ma
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Lu
authors.forename: Jainqiang
authors.initials: J
authors.lastname: Peng
authors.forename: Shuping
authors.initials: S
authors.lastname: Tan
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Yuan
authors.forename: Fang
authors.initials: F
authors.lastname: Yin
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Wu
authors.forename: Yunde
authors.initials: Y
authors.lastname: Hou
mesh_terms: D002460:Cell Line; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D008156:Luciferases / Q000096:biosynthesis* / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012313:RNA / Q000235:genetics*; D014162:Transfection
chemical_list: D012313:RNA; D008156:Luciferases