PCR-linked in vitro expression: a novel system for high-throughput construction and screening of protein libraries.
A novel entirely in vitro strategy for generation and screening of a combinatorial protein library in an array format has been developed and is experimentally demonstrated. The strategy exploits virtues of PCR and in vitro coupled transcription/translation. Our new approach provides high-throughput construction and screening of the in vitro protein library, and compatibility with various selection methods.
Rungpragayphan
Suang
S
Laboratory of Molecular Biotechnology, Graduate School of Biological and Agricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.
Nakano
Hideo
H
Yamane
Tsuneo
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D020650:Combinatorial Chemistry Techniques; D016133:Polymerase Chain Reaction / Q000379:methods*; D011506:Proteins / Q000737:chemistry*
D011506:Proteins