Apoptosis of leukemia cells induced by CD34+ cells transferred exogenous Fas ligand.
To assess the value of CD34+ cells transferred exogenous Fas ligand (FasL) in inducing apoptosis of human leukemic cells, the CD34+ cells transfected with FasL or without, pretreated with mitomycin C, was mixed with leukemic cell line U937 cells in presence or absence of daunorubicin (DNR) or cytosine arabinoside (Ara-C). After 18 h, apoptosis of cells was detected by FCM and TUNEL. Induced for 18 h by CD34+ cells transfected with FasL or without, the ratio of apoptosis of U937 cells was (5.0 +/- 1.3)%, (10.8 +/- 0.6)% (P < 0.01), respectively. Induced by FasL+ CD34+ + DNR, FasL+ CD34+ + Ara-C, the ratio was (13.4 +/- 1.0)% (P < 0.05), (17.9 +/- 1.3)% (P < 0.01), respectively. The result demonstrated that CD34+ cells transfected with exogenous FasL could induce apoptosis of human leukemic cells and showed a cytotoxic synergistic effect when used in combination with chemotherapeutic drugs, suggesting that it was possible to develop a new method in treatment of leukemia.
Xiao
Juan
J
Institute of Hematology, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022.
Zou
Ping
P
Liu
Zhongwen
Z
Hu
Zhongbo
Z
Liu
Lingbo
L
D018952:Antigens, CD34 / Q000032:analysis*; D017209:Apoptosis / Q000187:drug effects*; D002450:Cell Communication / Q000502:physiology; D003561:Cytarabine / Q000494:pharmacology; D018076:DNA, Complementary / Q000235:genetics; D003630:Daunorubicin / Q000494:pharmacology; D053222:Fas Ligand Protein; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics*; D016685:Mitomycin / Q000494:pharmacology; D014162:Transfection; D020298:U937 Cells; D019014:fas Receptor / Q000235:genetics / Q000378:metabolism
D018952:Antigens, CD34; D018076:DNA, Complementary; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D008562:Membrane Glycoproteins; D019014:fas Receptor; D003561:Cytarabine; D016685:Mitomycin; D003630:Daunorubicin