title: The telomeric region of BTA18 containing a potential QTL region for health in cattle exhibits high similarity to the HSA19q region in humans.
abstract: We have applied a targeted physical mapping approach, based on the isolation of bovine region-specific large-insert clones using homologous human sequences and chromosome microdissection, to enhance the physical gene map of the telomeric region of BTA18 and to prove its evolutionary conservation. The latter is a prerequisite to exploit the dense human gene map for future positional cloning approaches. Partial sequencing and homology search were used to characterize 20 BACs targeted to the BTA18q2.4-q2.6 region. We used fluorescence in situ hybridization (FISH) to create physical maps of 11 BACs containing 15 gene loci; these BACs served as anchor loci. Using these approaches, 12 new gene loci (CKM, STK13, PSCD2, IRF3, VASP, ACTN4, ITPKC, CYP2B6, FOSB, DMPK, MIA, SIX5) were assigned on BTA18 in the bovine cytogenetic map. A resolved physical map of BTA18q2.4-q2.6 was developed, which encompasses 28 marker loci and a comparative cytogenetic map that contains 15 genes. The mapping results demonstrate the high evolutionary conservation between the telomeric region of BTA18q and HSA19q.
authors.affiliation: Research Unit for Molecular Biology, Research Institute for the Biology of Farm Animals Dummerstorf, D-18196 Dummerstorf, Germany.
authors.forename: Ronald M
authors.initials: RM
authors.lastname: Brunner
authors.forename: Hanka
authors.initials: H
authors.lastname: Sanftleben
authors.forename: Tom
authors.initials: T
authors.lastname: Goldammer
authors.forename: Christa
authors.initials: C
authors.lastname: Kühn
authors.forename: Rosemarie
authors.initials: R
authors.lastname: Weikard
authors.forename: Srinivas R
authors.initials: SR
authors.lastname: Kata
authors.forename: James E
authors.initials: JE
authors.lastname: Womack
authors.forename: Manfred
authors.initials: M
authors.lastname: Schwerin
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D017931:DNA Primers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping / Q000662:veterinary*; D040641:Quantitative Trait Loci; D016615:Telomere
chemical_list: D017931:DNA Primers