[The method for determination of aconitum alkaloids in Radix Aconiti Kusnezoffii].
The methods for the determination of Aconitum alkaloids in Radix Aconiti Kusnezoffii were established. Volumetric analysis with the indicator of methyl red-bromcresol green and colorimetric analysis with acid dye of bromecresol green under the wavelength of 416 nm were used for the assay of total alkaloids. Colorimetric analysis of hydroxamic acid-Fe was for the assay of total ester-diterpene type Aconitum alkaloids. Three kinds of diester-diterpene type Aconitum alkaloids (aconitine, hypaconitine and mesaconitine) were determined by HPLC method. The HPLC system consisted of C18 as the stationary phase and acetonitrile: buffer (acetic acid-triethylamine, pH 6.25) = 71:29 as the mobile phase. The wavelength was 235 nm.
Huang
Jianming
J
School of Pharmacy, Fudan University, Shanghai 200032.
Guo
Jixian
J
Sun
Mingming
M
Lu
Bin
B
Duan
Gengli
G
Chen
Wansheng
W
D000157:Aconitine / Q000031:analogs & derivatives* / Q000032:analysis*; D031841:Aconitum / Q000737:chemistry*; D000470:Alkaloids / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003124:Colorimetry / Q000379:methods; D004396:Coloring Agents; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D010598:Pharmacognosy
D000470:Alkaloids; D004396:Coloring Agents; D004365:Drugs, Chinese Herbal; C058309:hypaconitine; C019470:mesaconitine; D000157:Aconitine