Alteration in the expression of early stage processing enzymes of N-glycan during myeloid and monocytoid differentiation of HL-60 cells.
The expressions of the enzymes participating in the early stage of N-glycan processing, Golgi alpha-Mase-I, alpha-Mase-II and GnT-I, GnT-II, were studied before and after HL-60 cells were differentiated to myelocytes or monocytes induced by ATRA or PMA, respectively. It was found that alpha-Mase-I activity and GnT-I mRNA were decreased by both ATRA and PMA, while alpha-Mase-II and GnT-II were altered insignificantly. The down-regulation of alpha-Mase-I and GnT-I was cell specific, since ATRA up-regulated alpha-Mase-I and GnT-I in the H7721 hepatocarcinoma cell line. However, in H7721 cells, PMA also decreased alpha-Mase-I and GnT-I, and both ATRA and PMA also did not obviously change the expressions of alpha-Mase-II and GnT-II.
Zhao
Jia-Hong
JH
Key Laboratory of Glycoconjugate Research, Department of Biochemistry, Ministry of Health, School of Medicine, Fudan University, 200032, Shanghai, China.
Zhang
Ying
Y
Zhang
Xia-Ying
XY
Chen
Hui-Li
HL
D015152:Blotting, Northern; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D018076:DNA, Complementary; D015536:Down-Regulation; D006056:Golgi Apparatus / Q000201:enzymology*; D018922:HL-60 Cells / Q000166:cytology / Q000201:enzymology; D006801:Humans; D008361:Mannosidases / Q000235:genetics / Q000378:metabolism*; D009000:Monocytes / Q000201:enzymology; D022423:Myeloid Cells / Q000201:enzymology; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology; D015854:Up-Regulation
D018076:DNA, Complementary; D011134:Polysaccharides; D012333:RNA, Messenger; D014212:Tretinoin; D017351:N-Acetylglucosaminyltransferases; C024850:alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase I; C063312:beta-1,3-galactosyl-0-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase; D008361:Mannosidases; C046090:mannosyl-oligosaccharide 1,2-alpha-mannosidase; C038829:mannosyl-oligosaccharide 1,3 - 1,6-alpha-mannosidase; D013755:Tetradecanoylphorbol Acetate