Investigation of the influence on conformational transition of DNA induced by cationic lipid vesicles.
Recent studies have focused on the structural features of DNA-lipid assemblies. In this paper we take nile blue A (NBA) as a probe molecule to study the influence of the conformational transition of DNA induced by didodecyldimethylammonium bromide (DDAB) cationic vesicles to the interaction between DNA and the probe molecules. We find that upon binding to DNA, a secondary conformational transition of DNA induced by the cationic liposome from the native B-form to the C-form resulted in the change of binding modes of NBA to DNA and different complexes are formed between DNA, DDAB and NBA.
Zhang
Zheling
Z
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, People's Republic of China.
Huang
Weimin
W
Wang
Erkang
E
Dong
Shaojun
S
D000818:Animals; D002412:Cations; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000032:analysis / Q000737:chemistry*; D004563:Electrochemistry; D005456:Fluorescent Dyes / Q000494:pharmacology; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D010078:Oxazines / Q000494:pharmacology; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D013053:Spectrophotometry; D013950:Thymus Gland / Q000378:metabolism; D014466:Ultraviolet Rays
D002412:Cations; D005456:Fluorescent Dyes; D008055:Lipids; D010078:Oxazines; D000644:Quaternary Ammonium Compounds; C008619:Nile Blue; C015831:dimethyldioctadecylammonium; D004247:DNA