title: [In vitro culture of murine fetal epidermal stem cell and its relationship with the regeneration of follicle].
abstract: OBJECTIVE
  To isolate and culture the murine fetal epidermal stem cells (ESCs) and folliculus pili cells (FPCs) in vitro, and to observe the regeneration of hair follicle and epidermis after cografting of ESCs and FPCs.
  
  
  METHODS
  The ESCs were isolated by adhering to murine type IV collagen and were cultured in conditional medium. The expression level of beta1-integrin and keratin 15 in ESCs was detected. At the same time, the cell cycle and clony forming eficiency (CFE) in ESCs were also determined. The FPCs were isolated and cultured and inoculated in fibrin-gel to form FPCs-gel. A full skin equivalent was prepared by combining ESCs with FPCs-gel and was grafted onto total skin loss wounds on the back of BALB/C nude mice. The histological changes of the wounds and the hair follicles were observed at 8 - 10 weeks after the grafting.
  
  
  RESULTS
  There were high level expressions of beta1-integrin and keratin 15 in murine fetal ESCs. It was indicated by cell cycle analysis that cells in G1 stage accounted for 94.9% of the cells, while that in S stage, 3.5%, suggesting slow cell cycle. Nevertheless, the keratinocytes in G1 stage accounted for 74.1% and that in S stage, 17.5% of cells in control group. The CFE of ESCs was 15.3%, and it was much higher than that in control group (6.7%). The newly formed hair follicles could be found in the grafted rats but not in the control group 8 - 10 weeks after the wound healing in nude rats.
  
  
  CONCLUSIONS
  The ESCs could be successfully isolated and cultured in vitro and might participate in the formation of hair follicle structure under the induction of FPCs.
authors.affiliation: Department of Burns, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032. Shaanxi Province. P.R. China.
authors.forename: Jun-Tao
authors.initials: JT
authors.lastname: Han
authors.forename: Bi
authors.initials: B
authors.lastname: Chen
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Zhang
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
authors.forename: Feng
authors.initials: F
authors.lastname: Li
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D062109:Dermatologic Surgical Procedures; D004817:Epidermis; D005333:Fetus; D018859:Hair Follicle / Q000166:cytology / Q000502:physiology*; D007150:Immunohistochemistry; D019012:Integrin beta1 / Q000032:analysis; D053569:Keratin-15; D007633:Keratins / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D012038:Regeneration; D012867:Skin / Q000293:injuries; D016038:Skin Transplantation / Q000379:methods; D017768:Skin, Artificial; D013234:Stem Cells / Q000737:chemistry / Q000166:cytology*; D014945:Wound Healing
chemical_list: D019012:Integrin beta1; D053569:Keratin-15; C492053:Krt15 protein, mouse; D007633:Keratins