In vitro effects of dantrolene sodium and verapamil on noradrenaline-induced contractions of rabbit aorta and their interactions.
The effects of dantrolene sodium (0.1 to 10 microM) and verapamil (0.01 to 1 microM) administered alone or together (1 microM verapamil, 0.1 to 10 microM dantrolene sodium) were investigated in isolated rabbit thoracic aorta precontracted with 0.1 microM noradrenaline (NA). Verapamil plus dantrolene sodium produced a dose-dependent inhibition of aortic strips contractions evoked by NA, and all concentrations of dantrolene sodium significantly decreased the inhibitory effect of 1 microM verapamil (p < 0.001, ANOVA). In conclusion, dantrolene sodium and verapamil inhibited 0.1 microM noradrenaline-evoked aorta contractions, and all doses of dantrolene sodium decreased the inhibitory effect of 1 microM verapamil in a dose-dependent manner.
Büyükokuroğlu
Mehmet Emin
ME
Atatürk University, Medical Faculty, Department of Pharmacology, Erzurum, Turkey. memin@atauni.edu.tr
Göçer
Fatma
F
Açikel
Mahmut
M
Gepdiremen
Akçahan
A
D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D003620:Dantrolene / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000187:drug effects; D009125:Muscle Relaxants, Central / Q000494:pharmacology*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D009638:Norepinephrine / Q000037:antagonists & inhibitors*; D011817:Rabbits; D014662:Vasoconstrictor Agents / Q000037:antagonists & inhibitors*; D014700:Verapamil / Q000494:pharmacology*
D002121:Calcium Channel Blockers; D009125:Muscle Relaxants, Central; D014662:Vasoconstrictor Agents; D014700:Verapamil; D003620:Dantrolene; D009638:Norepinephrine