title: [The role of dendritic cell and macrophage in hepatoma antigen-presenting].
abstract: OBJECTIVE
  To study the role of dendritic cells (DCs) and macrophages, differentiated from the same individual peripheral blood monocytes, in tumor antigen- presenting.
  
  
  METHODS
  DCs and macrophages were differentiated from human peripheral blood monocytes by adding both Granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) or GM-CSF only. Then they were loaded with tumor antigen at different concentrations and cocultured with autologous T cells in 96-well flat-bottomed microtiter plates for five days at 37 degrees C, 5% CO(2). (3)H-thymine was added before the culture terminated, and twelve hours later, the cells were gathered to test the cpm value.
  
  
  RESULTS
  Both DCs and macrophages chased with tumor antigen could strongly stimulate the proliferation of autologous T cells, especially DCs. The stimulation effect with 20 microl/ml antigen was the most remarkable and the cmp values were 11,950.3 +/-1621.8, 8,708.5 +/-176.1, 402.5+/-43.1 in DCs group, Macrophages group, and lymphocytes group, respectively.
  
  
  CONCLUSIONS
  The antigen presenting role of DCs is stronger than that of macrophages from the same individual.
authors.affiliation: Institute of Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China.
authors.forename: Yong-guo
authors.initials: YG
authors.lastname: Li
authors.forename: Yun-ping
authors.initials: YP
authors.lastname: Luo
authors.forename: Zeng-wei
authors.initials: ZW
authors.lastname: Liang
authors.forename: Da-chuan
authors.initials: DC
authors.lastname: Cai
authors.forename: Ying-hua
authors.initials: YH
authors.lastname: Lan
authors.forename: Qun
authors.initials: Q
authors.lastname: Xie
authors.forename: Hong
authors.initials: H
authors.lastname: Ren
mesh_terms: D017951:Antigen Presentation / Q000276:immunology; D000938:Antigen-Presenting Cells / Q000276:immunology / Q000502:physiology*; D000951:Antigens, Neoplasm / Q000276:immunology; D006528:Carcinoma, Hepatocellular / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology / Q000502:physiology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D008113:Liver Neoplasms / Q000276:immunology*; D008264:Macrophages / Q000276:immunology / Q000502:physiology*; D014407:Tumor Cells, Cultured
chemical_list: D000951:Antigens, Neoplasm; D016178:Granulocyte-Macrophage Colony-Stimulating Factor