title: Activity of human monocytes in IgE antibody-dependent surveillance and killing of ovarian tumor cells.
abstract: We have previously shown that a chimeric IgE antibody against the folic acid receptor (MOv18 IgE) inhibits tumor growth in a SCID mouse model of ovarian carcinoma. MOv18 IgE gave greater protection than the corresponding chimeric MOv18 IgG1. We have now confirmed these effects in a nude-mouse model of ovarian carcinoma and have demonstrated for the first time that human monocytes are active in IgE antibody-dependent cell-mediated cytotoxicity. Injection of tumor-bearing mice with PBMC and MOv18 IgE led to infiltration of monocytes into the tumors and prolonged survival of the mice. Incubation of PBMC or purified monocytes and MOv18 IgE with ovarian tumor cells in vitro resulted in tumor cell killing proportional to the expression of unoccupied FcepsilonRI on monocytes.We observed phagocytosis of tumor cells by the monocytes in vitro. Our results suggest that tumor-specific IgE antibodies may be exploited for immunotherapy of cancer.
authors.affiliation: The Randall Centre, King's College London, London, GB.
authors.forename: Sophia N
authors.initials: SN
authors.lastname: Karagiannis
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
authors.forename: Nick
authors.initials: N
authors.lastname: East
authors.forename: Frances
authors.initials: F
authors.lastname: Burke
authors.forename: Stephane
authors.initials: S
authors.lastname: Riffard
authors.forename: Marguerite G
authors.initials: MG
authors.lastname: Bracher
authors.forename: Richard G
authors.initials: RG
authors.lastname: Thompson
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Durham
authors.forename: Lawrence B
authors.initials: LB
authors.lastname: Schwartz
authors.forename: Frances R
authors.initials: FR
authors.lastname: Balkwill
authors.forename: Hannah J
authors.initials: HJ
authors.lastname: Gould
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D058846:Antibodies, Monoclonal, Murine-Derived; D000920:Antibody-Dependent Cell Cytotoxicity; D001491:Basophils / Q000276:immunology; D002277:Carcinoma / Q000276:immunology* / Q000473:pathology; D002465:Cell Movement; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000235:genetics / Q000276:immunology*; D007074:Immunoglobulin G / Q000235:genetics / Q000276:immunology; D007157:Immunologic Surveillance; D051379:Mice; D008819:Mice, Nude; D009000:Monocytes / Q000276:immunology*; D010051:Ovarian Neoplasms / Q000276:immunology* / Q000473:pathology; D017455:Receptors, IgE / Q000378:metabolism; D017452:Receptors, IgG / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000276:immunology; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
chemical_list: D000911:Antibodies, Monoclonal; D058846:Antibodies, Monoclonal, Murine-Derived; D007074:Immunoglobulin G; C424863:MOv18 monoclonal antibody; D017455:Receptors, IgE; D017452:Receptors, IgG; D011993:Recombinant Fusion Proteins; D007073:Immunoglobulin E