title: [Separation of eleutheroside E from crude extract of Radix Acanthopanacis Senticosus by analytical and preparative high-speed countercurrent chromatography].
abstract: Analytical high-speed countercurrent chromatography (HSCCC) was used for the systematic optimization of the two-phase solvent system to separate eleutheroside E from crude extract of Radix Acanthopanacis Senticosus. Eleutheroside E was obtained by preparative HSCCC with two-phase solvent system composed of chloroform-methanol-isopropanol-water (5:6:1:4 in volume ratio). The mobile phase is the lower phase and operated at a flow-rate of 2.0 mL/min, while the apparatus rotated at 800 r/min. High performance liquid chromatographic analysis of eleutheroside E revealed that its purity was over 98%. HSCCC is a useful method for the separation of natural products.
authors.affiliation: Beijing Research Center for Separation & Purification Technology of Natural Products, Beijing Institute of New Technology Application, Beijing 100035, China. weiyun0570@sina.com
authors.forename: Yun
authors.initials: Y
authors.lastname: Wei
authors.forename: Tian-you
authors.initials: TY
authors.lastname: Zhang
authors.forename: Ke-you
authors.initials: KY
authors.lastname: Wu
mesh_terms: D003377:Countercurrent Distribution / Q000379:methods*; D028001:Eleutherococcus / Q000737:chemistry*; D005960:Glucosides / Q000302:isolation & purification*; D017705:Lignans; D018517:Plant Roots / Q000737:chemistry
chemical_list: D005960:Glucosides; D017705:Lignans; C421885:eleutheroside E