Species-specific class I gene expansions formed the telomeric 1 mb of the mouse major histocompatibility complex.
We have determined the complete sequence of 951,695 bp from the class I region of H2, the mouse major histocompatibility complex (Mhc) from strain 129/Sv (haplotype bc). The sequence contains 26 genes. The sequence spans from the last 50 kb of the H2-T region, including 2 class I genes and 3 class I pseudogenes, and includes the H2-M region up to Gabbr1. A 500-kb stretch of the H2-M region contains 9 class I genes and 4 pseudogenes, which fall into two subfamilies, M1 and M10, distinct from other mouse class I genes. This M1/M10 class I gene-cluster is separated from the centromeric H2-T and the telomeric H2-M4, -5 and -6 class I genes by "nonclass I genes". Comparison with the corresponding 853-kb region of the human Mhc, which includes the HLA-A region, shows a mosaic of conserved regions of orthologous nonclass I genes separated by regions of species-specific expansion of paralogous Mhc class I genes. The analysis of this mosaic structure illuminates the dynamic evolution of the Mhc class I region among mammals and provides evidence for the framework hypothesis.
Takada
Toyoyuki
T
Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.
Kumánovics
Attila
A
Amadou
Claire
C
Yoshino
Masayasu
M
Jones
Elsy P
EP
Athanasiou
Maria
M
Evans
Glen A
GA
Fischer Lindahl
Kirsten
K
D000595:Amino Acid Sequence / Q000235:genetics; D000818:Animals; D001482:Base Composition / Q000235:genetics; D002503:Centromere / Q000235:genetics; D016000:Cluster Analysis; D019295:Computational Biology / Q000379:methods / Q000706:statistics & numerical data; D019143:Evolution, Molecular; D016678:Genome; D006183:H-2 Antigens / Q000235:genetics*; D000949:Histocompatibility Antigens Class II / Q000737:chemistry / Q000235:genetics; D006801:Humans; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods; D013045:Species Specificity
D006183:H-2 Antigens; C416189:H2-M antigens; D000949:Histocompatibility Antigens Class II