[Synergistic effect of toremifene and cisplatin on human lung cancer cell line A549].
OBJECTIVE
To study the toxic effect of toremifene (TOR) and its synergistic effect with cisplatin (DDP) on human lung adenocarcinoma cell line A549.


METHODS
The cytotoxic effects of these agents on human lung cancer cell line A549 were monitored by a tetrazolium-based colorimetric assay (MTT assay). The cell cycle and DNA content were detected by flow cytometer technic. p21 expression level was monitored by Western blot.


RESULTS
Toremifene inhibited the growth of A549 cell, with > or = 5 micromol/L significantly enhancing the chemosensitivity of cisplatin. TOR enhanced the antitumor activity of DDP at S, G(2) and M phases of cells. And p21 expression was increased after TOR and DDP had been given.


CONCLUSIONS
Toremifene (> or = 5 micromol/L) combined with cisplatin shows significant synergistic anti-tumor effect on A549 cells.
Zhang
Xueyan
X
Department of Respiratory Medicine, Chang Hai Hospital, Second Military Medical University, Shanghai 200433, China.
Li
Qiang
Q
Han
Yiping
Y
Liu
Zhongling
Z
D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm; D004354:Drug Screening Assays, Antitumor; D004357:Drug Synergism; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D017312:Toremifene / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D017312:Toremifene; D002945:Cisplatin