Lycopene attenuates arachidonic acid toxicity in HepG2 cells overexpressing CYP2E1.
Arachidonic acid (AA) was shown to be toxic to HepG2 cells expressing cytochrome P4502E1 (CYP2E1) because of oxidative stress. The aim of this study was to investigate whether lycopene, a carotenoid with high anti-oxidant capacity, protects HepG2 cells expressing CYP2E1 against AA toxicity. In preliminary experiments, lycopene as well as placebo (vehicle) were not toxic in the three types of cells tested: HepG2 cells, HepG2 cells transfected with pCI-neo (Neo) or pCI-neo/2E1 (2E1). AA produced toxic effects, especially in the 2E1 cells, and caused a remarkable increase in hydrogen peroxide production and lipid peroxidation compared to the Neo and HepG2 cells. Lycopene had a protective effect whereas the placebo did not. This was due, at least in part, to inhibition of hydrogen peroxide production and of the resulting lipid peroxidation, confirming the potent anti-oxidant properties of lycopene and its suitability for clinical studies.
Xu
Youqing
Y
Alcohol Research and Treatment Center, Section of Liver Disease and Nutrition, Veterans Affairs Medical Center (151-2), Mt Sinai School of Medicine, 130 West Kingsbridge Rd, Bronx, NY, USA.
Leo
Maria A
MA
Lieber
Charles S
CS
D016718:Arachidonic Acid / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D002338:Carotenoids / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics / Q000378:metabolism*; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D000077276:Lycopene; D014161:Transduction, Genetic
D016718:Arachidonic Acid; D002338:Carotenoids; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D019392:Cytochrome P-450 CYP2E1; D000077276:Lycopene