FGFR1 is independently required in both developing mid- and hindbrain for sustained response to isthmic signals.
Fibroblast growth factors (FGFs) are signaling molecules of the isthmic organizer, which regulates development of the midbrain and cerebellum. Tissue-specific inactivation of one of the FGF receptor (FGFR) genes, Fgfr1, in the midbrain and rhombomere 1 of the hindbrain of mouse embryos results in deletion of the inferior colliculi in the posterior midbrain and vermis of the cerebellum. Analyses of both midbrain-hindbrain and midbrain-specific Fgfr1 mutants suggest that after establishment of the isthmic organizer, FGFR1 is needed for continued response to the isthmic signals, and that it has direct functions on both sides of the organizer. In addition, FGFR1 appears to modify cell adhesion properties critical for maintaining a coherent organizing center. This may be achieved by regulating expression of specific cell-adhesion molecules at the midbrain-hindbrain border.
Trokovic
Ras
R
Institute of Biotechnology, Viikki Biocenter, PO Box 56, 00014 University of Helsinki, Finland.
Trokovic
Nina
N
Hernesniemi
Sanna
S
Pirvola
Ulla
U
Vogt Weisenhorn
Daniela M
DM
Rossant
Janet
J
McMahon
Andrew P
AP
Wurst
Wolfgang
W
Partanen
Juha
J
D000818:Animals; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D002448:Cell Adhesion / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D004622:Embryo, Mammalian / Q000033:anatomy & histology / Q000502:physiology*; D036386:Ephrin-A5 / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D005865:Gestational Age; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D017403:In Situ Hybridization; D019426:Integrases / Q000235:genetics / Q000378:metabolism; D008636:Mesencephalon / Q000033:anatomy & histology / Q000254:growth & development* / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D009043:Motor Activity / Q000502:physiology; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D051496:Receptor, Fibroblast Growth Factor, Type 1; D051497:Receptor, Fibroblast Growth Factor, Type 2; D017468:Receptors, Fibroblast Growth Factor / Q000235:genetics / Q000378:metabolism*; D012249:Rhombencephalon / Q000033:anatomy & histology / Q000254:growth & development* / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D051153:Wnt Proteins; D029961:Zebrafish Proteins
D015820:Cadherins; C504494:Cdh22 protein, mouse; C080330:En1 protein, mouse; D036386:Ephrin-A5; D018398:Homeodomain Proteins; D011518:Proto-Oncogene Proteins; D017468:Receptors, Fibroblast Growth Factor; D014764:Viral Proteins; D051153:Wnt Proteins; D029961:Zebrafish Proteins; C496349:Fgfr1 protein, mouse; C496354:Fgfr2 protein, mouse; D020794:Receptor Protein-Tyrosine Kinases; D051496:Receptor, Fibroblast Growth Factor, Type 1; D051497:Receptor, Fibroblast Growth Factor, Type 2; C045073:Cre recombinase; D019426:Integrases