Intracellular localisation of Fv1.
Retroviral resistance mediated by the murine Fv1 gene is believed to result from a direct interaction between the Fv1 gene product and the viral capsid protein. To study the mechanism of Fv1 action, the expression and intracellular localisation of the Fv1 protein were examined. Only very low levels of protein expression seem necessary for virus restriction but the site of expression appears crucial. Active Fv1 was found in association with tubules of the trans-Golgi network, whereas an inactive form was localised in the endoplasmic reticulum. We hypothesize that Fv1 is compartmentalised in the cell on the pathway taken by virus en route to the nucleus, suggesting that incoming virus must pass the trans-Golgi network during its transit to the nucleus.
Yap
Melvyn W
MW
Division of Virology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom.
Stoye
Jonathan P
JP
D016475:3T3 Cells; D000199:Actins / Q000187:drug effects / Q000235:genetics; D000818:Animals; D000911:Antibodies, Monoclonal; D015638:Cytochalasin D / Q000494:pharmacology; D003599:Cytoskeleton / Q000821:virology; D051379:Mice; D008870:Microtubules / Q000187:drug effects; D009115:Muridae; D011506:Proteins / Q000032:analysis* / Q000235:genetics; D011994:Recombinant Proteins / Q000032:analysis; D014162:Transfection
D000199:Actins; D000911:Antibodies, Monoclonal; C103189:Fv1 protein, mouse; D011506:Proteins; D011994:Recombinant Proteins; D015638:Cytochalasin D