title: Genetic map of AFLP markers in the rat (Rattus norvegicus) derived from the H x B/Ipcv and B x H/Cub sets of recombinant inbred strains.
abstract: The amplified fragment length polymorphism (AFLP) technique has been used to enhance marker density in a large set of recombinant inbred strains (H x B and B x H) derived from a spontaneously hypertensive rat (SHR/OlaIpcv) and a Brown-Norway (BN.lx/Cub) inbred strain. Thirteen different primer combinations were tested and a total of 191 polymorphic bands were detected. From these polymorphic bands 89 AFLP markers could be assigned to specific chromosomes. Several of these AFLP markers were mapped to regions with low marker density, thus filling up gaps in the existing genetic map of these recombinant inbred strains. These results substantiate the value of the AFLP technology in increasing marker density in genetic maps.
authors.affiliation: Department of Laboratory Animal Science, Faculty of Veterinary Medicine, Graduate School of Animal Health, Utrecht University, Utrecht, The Netherlands. a.bonne@antrg.azn.nl
authors.forename: Anita C M
authors.initials: AC
authors.lastname: Bonné
authors.forename: Maria G
authors.initials: MG
authors.lastname: den Bieman
authors.forename: Gert F
authors.initials: GF
authors.lastname: Gillissen
authors.forename: Vladimír
authors.initials: V
authors.lastname: Kren
authors.forename: Drahomíra
authors.initials: D
authors.lastname: Krenová
authors.forename: Vlasta
authors.initials: V
authors.lastname: Bílá
authors.forename: Václav
authors.initials: V
authors.lastname: Zídek
authors.forename: Vlastimil
authors.initials: V
authors.lastname: Kostka
authors.forename: Alena
authors.initials: A
authors.lastname: Musilová
authors.forename: Michal
authors.initials: M
authors.lastname: Pravenec
authors.forename: Bert F M
authors.initials: BF
authors.lastname: van Zutphen
authors.forename: Hein A
authors.initials: HA
authors.lastname: van Lith
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D015246:Deoxyribonuclease EcoRI / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers; D008297:Male; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D051381:Rats; D011914:Rats, Inbred BN; D011918:Rats, Inbred SHR; D011919:Rats, Inbred Strains / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D015246:Deoxyribonuclease EcoRI; D015252:Deoxyribonucleases, Type II Site-Specific; C027046:TCGA-specific type II deoxyribonucleases