Detection of single cells of Cryptococcus neoformans in clinical samples by solid-phase cytometry.
A method based on solid-phase cytometry for the detection and enumeration of single cells of Cryptococcus neoformans in serum and cerebrospinal fluid is described. Both viable and nonviable cells are detected by using fluorescence viability labeling and immunofluorescence. This 30-min procedure has a detection limit of 3 to 6 cells per ml.
Bauters
T G M
TG
Laboratory for Pharmaceutical Microbiology, Department of Pharmaceutical Analysis, Ghent University, Ghent, Belgium.
Swinne
D
D
Stove
V
V
Nelis
H J
HJ
D001769:Blood / Q000382:microbiology; D002555:Cerebrospinal Fluid / Q000382:microbiology; D003453:Cryptococcosis / Q000175:diagnosis* / Q000382:microbiology; D003455:Cryptococcus neoformans / Q000166:cytology* / Q000254:growth & development / Q000302:isolation & purification*; D005434:Flow Cytometry / Q000379:methods*; D005455:Fluorescent Antibody Technique; D006801:Humans; D016919:Meningitis, Cryptococcal / Q000175:diagnosis / Q000382:microbiology; D013997:Time Factors