Effect of polyethylene glycol on gene delivery of polyethylenimine.
Polyethylene glycol (PEG) has been coupled to many cationic polymers such as polyethylenimine (PEI) to improve the stability and transfection efficiency. We prepared PEG-grafted PEI with different lengths and amounts of PEG and used these graft copolymers as nonviral gene vectors. We measured the complex size and zeta-potential of polymer-DNA complexes in the presence of salt to estimate the stability of polymer-DNA complexes. We also investigated the cytotoxicity and transfection efficiency in C3 cells. In the case of graft copolymers, the stability of polymer-DNA complexes and transfection efficiency were affected by the graft length and amount of PEG side chain. PEG side chains stabilize the polymer-DNA complexes in the presence of salt, but the longer PEG side chains also interrupt the gene delivery in the cells due to the more efficient steric hindrance by longer PEG side chains, and therefore the transfection efficiency is decreased. Short PEG side chains with molecular weight of 350 kDa stabilized the polymer-DNA complexes without decreased transfection efficiency.
Sung
Shi-Joon
SJ
Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Daejon.
Min
Sang Hyun
SH
Cho
Kuk Young
KY
Lee
Seongnam
S
Min
Youn-Jin
YJ
Yeom
Young Il
YI
Park
Jung-Ki
JK
D000818:Animals; D045744:Cell Line, Tumor / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D016503:Drug Delivery Systems / Q000379:methods*; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D010316:Particle Size; D011092:Polyethylene Glycols / Q000008:administration & dosage* / Q000737:chemistry; D011094:Polyethyleneimine / Q000008:administration & dosage* / Q000737:chemistry
D011092:Polyethylene Glycols; D011094:Polyethyleneimine