title: Ribonucleotide reductase immunoreactivity in adenocarcinoma cells and malignant or reactive mesothelial cells in serous effusions.
abstract: OBJECTIVE
  Ribonucleotide reductase (RNR) is a cytoplasmic enzyme that is essential for DNA synthesis. Its activity is strongly associated with cell proliferation. We assessed the value of immunostaining for RNR in distinguishing between reactive mesothelia (RM), malignant mesotheliomas (MM) and adenocarcinomas (AC) in serous effusions.
  
  
  METHODS
  Cytocentrifuged cell smears of serous effusions from 38 RM, 10 MM and 36 AC were immunostained with the monoclonal antibody KM1054 raised against the R2 subunit of RNR (RNR-R2) using the labeled streptavidin-biotin method. Quantitative RNR-R2 values were determined by counting the percentages of immunoreactive cells.
  
  
  RESULTS
  RNR-R2 immunostaining was confined to the cytoplasm. The median RNR-R2 value was 1.4% (range, 0-7.9%) for RM, 11.2% (4.1-15.3%) for MM and 12.1% (2.0-40.6%) for AC. Significant differences in RNR-R2 values were found for both AC versus RM (P < .001) and MM versus RM (P = .009). There was no difference between AC and MM (P = .26). An RNR-R2 value > or = 7% was found in 30 of 36 AC, 8 of 10 MM and 2 of 38 RM.
  
  
  CONCLUSIONS
  RNR-R2 immunostaining can be useful as an adjunct for differentiating AC or MM from RM in serous effusions.
authors.affiliation: First Department of Pathology, Yamaguchi University School of Medicine, Ube, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Okamura
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Kamei
authors.forename: Nobuo
authors.initials: N
authors.lastname: Sakuma
authors.forename: Nobuo
authors.initials: N
authors.lastname: Hanai
authors.forename: Tokuhiro
authors.initials: T
authors.lastname: Ishihara
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology* / Q000473:pathology*; D000918:Antibody Specificity / Q000276:immunology; D014408:Biomarkers, Tumor / Q000032:analysis; D002471:Cell Transformation, Neoplastic / Q000473:pathology; D003937:Diagnosis, Differential; D004848:Epithelium / Q000201:enzymology* / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation / Q000473:pathology; D008654:Mesothelioma / Q000201:enzymology* / Q000473:pathology*; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D012264:Ribonucleotide Reductases / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D012264:Ribonucleotide Reductases; C108427:ribonucleotide reductase R2 subunit