Murine gammaherpesvirus (MHV) M7 gene encoding glycoprotein 150 (gp150): difference in the sequence between 72 and 68 strains.
Murine gamma herpesvirus 72 (MHV-72) was isolated from the same species of free-living small rodent as MHV-68 which currently serves as a model for study of human gamma-herpesvirus pathogenesis. MHV-68 open reading frame (ORF) M7 encodes a virus-associated transmembrane glycoprotein 150 (gp150) and displays sequence homology with Epstein-Barr virus (EBV) membrane antigen gp350/220. MHV-68 was used to model potential efficacy of EBV gp350 as an immunogen to protect against virus-associated disease. Studies on MHV-72, which is considered as closely related to MHV-68, identified some dissimilarity from MHV-68. By the contrast to MHV-68, abnormal lymphocytes have been described after infection with MHV-72. We have therefore sequenced the MHV-72 gp150 gene to find out the evidence of difference from that of MHV-68. We show here that from five nucleotide mutations found four changed the codon. Three codon changes are mapped out of two gp150 transmembrane domains and out of proline rich repeat region, respectively. Possible changes in the predicted secondary structure are discussed.
Macáková
Katarína
K
Institute of Virology, Slovak Academy of Sciences, 842 45 Bratislava, Slovak Republic.
Matis
Ján
J
Rezuchová
Ingeborg
I
Kúdela
Otakar
O
Raslová
Hana
H
Kúdelová
Marcela
M
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D003411:Arvicolinae / Q000821:virology; D002460:Cell Line; D003062:Codon / Q000235:genetics; D004252:DNA Mutational Analysis; D005260:Female; D018142:Gammaherpesvirinae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D005814:Genes, Viral; D006023:Glycoproteins / Q000235:genetics*; D008321:Mammary Glands, Animal; D051379:Mice; D008969:Molecular Sequence Data; D009115:Muridae / Q000821:virology; D016366:Open Reading Frames; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D017433:Protein Structure, Secondary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014759:Viral Envelope Proteins / Q000235:genetics*; D014763:Viral Matrix Proteins / Q000737:chemistry; D015678:Viral Structural Proteins / Q000235:genetics
D003062:Codon; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D006023:Glycoproteins; D014759:Viral Envelope Proteins; D014763:Viral Matrix Proteins; D015678:Viral Structural Proteins