title: Intracellular visualization of tetrodotoxin (TTX) in the skin of a puffer Tetraodon nigroviridis by immunoenzymatic technique.
abstract: Micro-distributions of tetrodotoxin (TTX) in the skin of a brackish-water puffer Tetraodon nigroviridis were investigated by means of a monoclonal anti-TTX antibody under light and transmission electron microscope. In light microscopy TTX antigen was visualized as brown color in undifferentiated basal cells and succiform cells of the skin, while in electron microscopy TTX was detected as black dots of immunogold in lysosomes of basal cells. From the results, it can be inferred that when TTX from the blood plasma of T. nigroviridis enters into the undifferentiated basal cells through diffusion, it is taken to the lysosomes by phagocytosis and exists there through binding with internal constituent(s) of the organelles.
authors.affiliation: Graduate School of Science and Technology, Nagasaki University, Nagasaki 852-8521, Japan.
authors.forename: Yahia
authors.initials: Y
authors.lastname: Mahmud
authors.forename: Osamu
authors.initials: O
authors.lastname: Arakawa
authors.forename: Akitoyo
authors.initials: A
authors.lastname: Ichinose
authors.forename: Mohosena
authors.initials: M
authors.lastname: Begum Tanu
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Takatani
authors.forename: Kazumi
authors.initials: K
authors.lastname: Tsuruda
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kawatsu
authors.forename: Yonekazu
authors.initials: Y
authors.lastname: Hamano
authors.forename: Tamao
authors.initials: T
authors.lastname: Noguchi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D007124:Immunoenzyme Techniques; D012867:Skin / Q000378:metabolism*; D023721:Tetraodontiformes; D013779:Tetrodotoxin / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D013779:Tetrodotoxin