title: Effects of erythromycin on Pseudomonas aeruginosa adherence to collagen and morphology in vitro.
abstract: The airways of patients with bronchiectasis and cystic fibrosis are often chronically colonised by Pseudomonas aeruginosa (PA), which is virtually impossible to eradicate. Low-dose erythromycin (EM), for unknown mechanisms, is efficacious in bronchiectasis and diffuse panbronchiolitis. In this study, an in vitro model to investigate PA adherence to human type IV basement collagen was developed by using scanning electron microscopy (SEM). There were significantly less PA bacilli per 20 random SEM fields (4,000x) when PA was cultured in 0.05, 0.5 and 5 microg x mL(-1) of EM compared with control (absence of EM). Adherence density (20 SEM fields x log(-1) inocular size) for PA obtained from no EM (56.8 +/- 43.16) was significantly higher than that obtained from 0.05, 0.5, and 5 microg x mL(-1) EM (21.5 +/- 17.56, 23.3 +/- 16.65, and 21.4 +/- 12.65 respectively). By using SEM it was found that PA, when incubated in EM (0.05, 0.5, 5 microg x mL(-1)) had a significant reduction in its diagonal length, radius, height, volume and surface area. It is possible, therefore, that these misshaped Pseudomonas aeruginosa bacilli are more susceptible to host defence mechanisms, while at the same time less adherent to the basement membrane of the airway in vivo. Therefore, this could help explain the clinical efficacy of low-dose erythromycin therapy on patients with Pseudomonas aeoruginosa infection.
authors.affiliation: Dept of Medicine, The University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong SAR, China. kwttsang@hku.hk
authors.forename: K W
authors.initials: KW
authors.lastname: Tsang
authors.forename: P
authors.initials: P
authors.lastname: Ng
authors.forename: P L
authors.initials: PL
authors.lastname: Ho
authors.forename: S
authors.initials: S
authors.lastname: Chan
authors.forename: G
authors.initials: G
authors.lastname: Tipoe
authors.forename: R
authors.initials: R
authors.lastname: Leung
authors.forename: J
authors.initials: J
authors.lastname: Sun
authors.forename: J C
authors.initials: JC
authors.lastname: Ho
authors.forename: M S
authors.initials: MS
authors.lastname: Ip
authors.forename: W K
authors.initials: WK
authors.lastname: Lam
mesh_terms: D001422:Bacterial Adhesion / Q000187:drug effects*; D001682:Biological Availability; D003094:Collagen / Q000502:physiology; D017077:Culture Media, Conditioned; D004305:Dose-Response Relationship, Drug; D004917:Erythromycin / Q000494:pharmacology*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D011336:Probability; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000502:physiology*; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric
chemical_list: D017077:Culture Media, Conditioned; D004917:Erythromycin; D003094:Collagen