[Differential expression of RPL6/Taxreb107 in drug resistant gastric cancer cell line SGC7901/ADR and its correlation with multiple-drug resistance].
OBJECTIVE
To investigate the differential expression of RPL6/Taxreb107 between drug-resistant gastric cancer cell line SGC7901/ADR and gastric cancer cell line SGC7901 as well as its correlation with multiple-drug resistance (MDR) in gastric cancer cells.


METHODS
Total RNA was extracted from SGC7901 and SGC77901/ADR, with internal control RT-PCR, Northern blot, gene cloning and expression, construction of eukaryotic expression vector, gene transfection by electroporation. The accumulation and retention of ADR in transiently transfected cell was detected by flow cytometry.


RESULTS
The internal control RT-PCR and Northern blot showed high RPL6/Taxreb107 expression in SGC7901/ADR cell line. Sense and antisense eukaryonic expression vectors demonstrated by double enzyme digestion were successfully transfected into gastric cancer cell line SGC7901 and SGC7901/ADR respectively by electroporation. The accumulation and retention of ADR detected 48 hours after transfection showed that RPL6 gene had shown effect on drug resistance in gastric cancer cell.


CONCLUSIONS
The high expression of RPL6/Taxreb107 in drug resistant gastric cancer cell shows its correlation with multiple-drug resistance in gastric cancer.
Du
Jing-ping
JP
Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi'an 710033, China.
Jin
Xiao-hang
XH
Shi
Yong-quan
YQ
Cao
Yun-xin
YX
Zhao
Yan-qiu
YQ
Liu
Chang-Jiang
CJ
Yin
Fang
F
Hu
Wen-hua
WH
Chen
Bao-jun
BJ
Qiao
Tai-dong
TD
Fan
Dai-ming
DM
D004268:DNA-Binding Proteins / Q000378:metabolism*; D018432:Drug Resistance, Multiple / Q000502:physiology*; D019008:Drug Resistance, Neoplasm / Q000502:physiology*; D006801:Humans; D013223:Statistics as Topic; D013274:Stomach Neoplasms / Q000473:pathology; D014407:Tumor Cells, Cultured
D004268:DNA-Binding Proteins; C093544:tax-responsive-element-binding protein 107, mouse