Synthesis of the P-cluster inorganic core of nitrogenases.
The [8Fe-7S] core of the P-clusters in nitrogenases is unique among the known [Fe-S] clusters which are essential to electron-transfer processes in nature. The [8Fe-7S] cluster has been thought unstable and to exist only in protein environments. We found that this unusual [8Fe-7S] structure can be self-assembled from the reaction of Fe(II) bis-amide, tetramethylthiourea, 2,4,6-triisopropylbenzenethiol, and elemental sulfur in a specific mole ratio. The structure of the complex isolated therefrom closely resembles that of the reduced form (PN) of the P-clusters, while the 6Fe(II)2Fe(III) oxidation state was manifested by the Mössbauer study.
Ohki
Yasuhiro
Y
Department of Chemistry, Graduate School of Science and Research Center for Materials Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8602, Japan.
Sunada
Yusuke
Y
Honda
Masaru
M
Katada
Motomi
M
Tatsumi
Kazuyuki
K
D005296:Ferrous Compounds / Q000737:chemistry; D007501:Iron / Q000737:chemistry*; D008958:Models, Molecular; D009591:Nitrogenase / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D015204:Spectroscopy, Mossbauer; D013455:Sulfur / Q000737:chemistry*
D005296:Ferrous Compounds; D013455:Sulfur; D007501:Iron; D009591:Nitrogenase