Histopathological study of dental pulp tissue capped with enamel matrix derivative.
The purpose of this study was to examine the histopathological response of dental pulp tissue to enamel matrix derivative (EMD) used as a pulp capping material. Thirty-two teeth from two mongrel dogs were divided into four equal groups. One group served as controls, and the others were used for deep Class V cavity preparation followed by direct pulp capping with enamel matrix derivative. The treated teeth were extracted after 1, 4, and 8 weeks and prepared for histopathological examination by light microscopy. All teeth prepared after 4 and 8 weeks demonstrated an increase in tertiary dentin, suggesting that enamel matrix derivative exerts a considerable influence on odontoblasts and endothelial cells of capillaries in dental pulp tissue. These results imply that enamel matrix derivative used as a pulp capping material may play a role in the calcification of dental pulp tissue.
Ishizaki
Nelson Tatsunari
NT
Department of Endodontics, Showa University School of Dentistry, Tokyo, Japan.
Matsumoto
Koukichi
K
Kimura
Yuichi
Y
Wang
Xiaogu
X
Yamashita
Akinori
A
D000818:Animals; D002113:Calcification, Physiologic / Q000187:drug effects; D002196:Capillaries / Q000187:drug effects / Q000473:pathology; D003723:Dental Amalgam; D003736:Dental Cavity Lining; D003737:Dental Cavity Preparation / Q000145:classification; D003746:Dental Enamel Proteins / Q000627:therapeutic use*; D003782:Dental Pulp / Q000187:drug effects / Q000473:pathology*; D003785:Dental Pulp Capping; D003789:Dental Pulp Exposure / Q000628:therapy; D003793:Dental Restoration, Permanent; D003809:Dentin, Secondary / Q000187:drug effects / Q000473:pathology; D004285:Dogs; D004730:Endothelium, Vascular / Q000187:drug effects / Q000473:pathology; D009804:Odontoblasts / Q000187:drug effects / Q000473:pathology; D011897:Random Allocation; D013997:Time Factors; D015035:Zinc Oxide-Eugenol Cement
D003746:Dental Enamel Proteins; D015035:Zinc Oxide-Eugenol Cement; C109069:enamel matrix proteins; D003723:Dental Amalgam