Gelatin blends with alginate: gels for lipase immobilization and purification.
Blends of natural polysaccharide sodium alginate (5%) with gelatin (3%) cross-linked with glutaraldehyde provide beads with excellent compressive strength (8 x 10(4) Pa) and regular structure on treatment with calcium chloride. Lipases from porcine pancreas, Pseudomonas cepacia, and Candida rugosa were immobilized in such a blend with excellent efficiency. The immobilized enzymes were stable and were reused several times without significant loss of enzyme activity both in aqueous and reverse micellar media. The beads were functionalized with succinic anhydride to obtain beads with extra carboxylic acid groups. These functionalized beads were then successfully used for 7.4-fold purification of crude porcine pancreatic lipase in a simple operation of protein binding at pH 5 and release at pH 8.5.
Fadnavis
Nitin W
NW
Biotransformations Laboratory, Indian Institute of Chemical Technology, Habsiguda, Hyderabad 500 007, India. fadnavisnw@yahoo.com
Sheelu
Gurrala
G
Kumar
Bezavada Mani
BM
Bhalerao
Mahendra U
MU
Deshpande
Ashlesha A
AA
D000464:Alginates / Q000737:chemistry* / Q000648:ultrastructure; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D019245:Compressive Strength; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000302:isolation & purification; D005780:Gelatin / Q000737:chemistry* / Q000648:ultrastructure; D005782:Gels; D020723:Glucuronic Acid / Q000737:chemistry*; D006603:Hexuronic Acids / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification* / Q000648:ultrastructure; D008422:Materials Testing; D008863:Microspheres; D010179:Pancreas / Q000737:chemistry / Q000201:enzymology; D010316:Particle Size; D013552:Swine; D013696:Temperature
D000464:Alginates; D001672:Biocompatible Materials; D004800:Enzymes, Immobilized; D005782:Gels; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D005780:Gelatin; D008049:Lipase