Effect of temperature on ascorbate peroxidase activity and flowering of Arabidopsis thaliana ecotypes under different light conditions.
Four Arabidopsis thaliana ecotypes were grown at 14 degrees C and 22 degrees C under two light conditions (300 microE m-2 s-1 or 150 microE m-2 s-1) and the effect of temperature on their growth and flowering time was studied. Flowering occurred within 31 days (experimental period) at 22 degrees C, whereas a decrease in growth temperature resulted in a delay in flowering (63 days) under both light conditions. At 14 degrees C, membrane-bound APX (tAPX) activity decreased and total chlorophyll (Chl) content increased with growth under both light conditions. However, at 22 degrees C, the tAPX activity increased and total Chl content decreased with growth under both light conditions. These results suggest that at 22 degrees C oxidative stress was high under both light conditions and consequently Chl content decreased under stressful conditions or vice versa for all the four A. thaliana ecotypes studied. Under both the temperature and light conditions, soluble APX activity showed an irregular pattern of growth. The increase in tAPX activity, with growth only at 22 degrees C but not at 14 degrees C, suggests increased H2O2 formation in flowering plants at 22 degrees C for all the four A. thaliana ecotypes studied. Before flowering, the tAPX activity showed a significantly negative correlation with flowering time. Higher oxidative stress in the lower-latitude ecotypes might induce earlier flowering than the higher-latitude ecotypes. From these results, we propose a hypothesis that H2O2 is one of the possible factors in flower induction.
Lokhande
Shubhangi D
SD
The Institute of Low Temperature Science, Hokkaido University, Sapporo, 060-0819, Japan. shubha@pop.lowtem.hokudai.ac.jp
Ogawa
Ken'ichi
K
Tanaka
Ayumi
A
Hara
Toshihiko
T
D017360:Arabidopsis / Q000201:enzymology* / Q000254:growth & development* / Q000528:radiation effects; D060387:Ascorbate Peroxidases; D002734:Chlorophyll / Q000378:metabolism; D002980:Climate; D017753:Ecosystem; D035264:Flowers / Q000254:growth & development / Q000528:radiation effects; D008027:Light; D010544:Peroxidases / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors
D002734:Chlorophyll; D010544:Peroxidases; D060387:Ascorbate Peroxidases