Effect of Dendrostellera lessertii on the intracellular alkaline phosphatase activity of four human cancer cell lines.
The cytotoxicity of an alcoholic extract of Dendrostellera lessertii (Thymelaeaceae) and one of its purified components was evaluated using four different human cancer cell lines. The IC(50) of the crude extract was found to be 28, 33, 31 and 30 microl for K562, CCRF-CEM, HL-60 and LNCaP-FGC-10 cells, respectively. Each microliter of the crude extract corresponds to 0.02 mg of the plant powder material. Similarly the IC(50) of the purified component was 5 x 10(-9), 20 x 10(-9), 8 x 10(-9) and 8 x 10(-9)M for K562, CCRF-CEM, HL-60 and LNCaP-FGC-10 cells, respectively. In addition, besides significant reduction in cell proliferation rates, the crude extract, at a dose of 18 microl/ml and the purified component at a dose of 3.5 x 10(-9)M, were capable of enhancing the intracellular protein content of each of the cell lines by almost 160% while the intracellular alkaline phosphatase activity was increased up to 450%.
Sadeghi
Heibatollah
H
Institute of Biochemistry and Biophysics, University of Tehran, P.O. Box 13145-1384, Tehran, Iran.
Yazdanparast
Razieh
R
D000469:Alkaline Phosphatase / Q000378:metabolism*; D006801:Humans; D007938:Leukemia / Q000201:enzymology*; D008297:Male; D028321:Plant Preparations / Q000494:pharmacology*; D011471:Prostatic Neoplasms / Q000201:enzymology*; D029645:Thymelaeaceae; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000201:enzymology
D028321:Plant Preparations; D000469:Alkaline Phosphatase