title: [Effects of single-dose external gamma irradiation on rat thyroid status as observed during the year].
abstract: We studied the rat thyroid status depending on the dose of external radiation and the time passed after the exposure. The experiments were carried out on female albino Wistar rats. The doses absorbed amounted to 0.25; 0.5; 1.0; 2.0 and 5.0 Gy. The animals were decapitated after 3, 6, 24 hours and 7, 30, 180 and 365 days following the radiation. The blood serum was assayed for the contents of thyroxin (T4) and triiodothyronine (T3) using a radioimmunological technique. The liver tissue was assayed spectrophotometrically for the activity of thyroid-induced NADP malate dehydrogenase (NADP-MDH). No changes were found in the blood thyroid hormone contents within short periods after the radiation effect. After 6 hours the T4 levels was 1.2-1.3-fold decreased in the blood of rats receiving the radiation doses of 1.0; 2.0; and 5.0 Gy. After a day the T4 concentration was diminished by 1.21-193-fold in all the experimental animals independently of the radiation dose and that of T3--in 2.0 Gy--and 5.0 Gy--irradiated group. After 7 days following the radiation the T4 and T3 contents remained to be decreased by 1.37-1.75 fold and those of NADP-MDH--by 1.3-1.8-fold in all the animal groups. In a month, the low dose-treated animals (0.25, 0.5, 1.0 Gy) showed the level of thyroid hormones reduced to the control values, whereas the 2.0 and 5.0 Gy--treated rats demonstrated this reduction only by 6 months. The decreased concentration of blood thyroid hormones was due not to the activation of their peripheral metabolism, but, probably, to inhibition of their biosynthesis in thyroid cells under conditions of radiation-induced activation of oxidative stress.
authors.affiliation: Institute of Biochemistry, National Academy of Sciences of Belarus, Grodno, 230017 Belarus.
authors.forename: L I
authors.initials: LI
authors.lastname: Nadol'nik
authors.forename: Z V
authors.initials: ZV
authors.lastname: Netsetskaia
authors.forename: V V
authors.initials: VV
authors.lastname: Vinogradov
mesh_terms: D000818:Animals; D003037:Cobalt Radioisotopes; D003627:Data Interpretation, Statistical; D005260:Female; D005500:Follow-Up Studies; D005720:Gamma Rays; D008099:Liver / Q000201:enzymology; D008291:Malate Dehydrogenase / Q000032:analysis; D009929:Organ Size; D018384:Oxidative Stress; D011829:Radiation Dosage; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D013053:Spectrophotometry; D013961:Thyroid Gland / Q000528:radiation effects*; D013974:Thyroxine / Q000097:blood; D013997:Time Factors; D014284:Triiodothyronine / Q000097:blood
chemical_list: D003037:Cobalt Radioisotopes; D014284:Triiodothyronine; D008291:Malate Dehydrogenase; D013974:Thyroxine