title: Usefulness of RA and RO isoforms of common leukocyte antigen (CD45) for early distinction between normal and abnormal promyelocytes.
abstract: Normal promyelocytes express CD45RO, while acute promyelocytic leukemia (APL) blasts express CD45RA, both isoforms of common leukocyte antigen with mutually exclusive expression. Here we report a patient with accumulation of promyelocytes in the bone marrow and the diagnostic strategy is described along with the ability to quickly discriminate between normal and abnormal cells using the flow cytometric detection of expression of RA/RO isoforms of CD45.
authors.affiliation: Department of Onco-Hematology, San Giovanni di Dio e Ruggi d'Aragona Hospital, 84100 Salerno, Italy. gdarena@altavista.it
authors.forename: Giovanni
authors.initials: G
authors.lastname: D'Arena
authors.forename: Nicola
authors.initials: N
authors.lastname: Cascavilla
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Nunziata
authors.forename: Gianni
authors.initials: G
authors.lastname: Perla
authors.forename: Rosella
authors.initials: R
authors.lastname: Matera
authors.forename: Angelo Michele
authors.initials: AM
authors.lastname: Carella
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Pistolese
mesh_terms: D001854:Bone Marrow Cells / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D042381:Granulocyte Precursor Cells / Q000378:metabolism*; D006801:Humans; D016130:Immunophenotyping; D015473:Leukemia, Promyelocytic, Acute / Q000175:diagnosis* / Q000378:metabolism*; D017493:Leukocyte Common Antigens / Q000737:chemistry*; D008875:Middle Aged; D020033:Protein Isoforms
chemical_list: D020033:Protein Isoforms; D017493:Leukocyte Common Antigens