title: Calbindin D28K interacts with Ran-binding protein M: identification of interacting domains by NMR spectroscopy.
abstract: Calbindin D(28K) is an EF-hand containing protein that plays a vital role in neurological function. We now show that calcium-loaded calbindin D(28K) interacts with Ran-binding protein M, a protein known to play a role in microtubule function. Using NMR methods, we show that a peptide, LASIKNR, derived from Ran-binding protein M, interacts with several regions of the calcium-loaded protein including the amino terminus and two other regions that exhibit conformational exchange on the NMR timescale. We suggest that the interaction between calbindin D(28K) and Ran-binding protein M may be important in calbindin D(28K) function.
authors.affiliation: Department of Biochemistry, Research Center, Mayo Clinic and Foundation, 200 First Street SW, Rochester, MN 55905, USA.
authors.forename: Ward
authors.initials: W
authors.lastname: Lutz
authors.forename: Elena M
authors.initials: EM
authors.lastname: Frank
authors.forename: Theodore A
authors.initials: TA
authors.lastname: Craig
authors.forename: Richele
authors.initials: R
authors.lastname: Thompson
authors.forename: Ronald A
authors.initials: RA
authors.lastname: Venters
authors.forename: Doug
authors.initials: D
authors.lastname: Kojetin
authors.forename: John
authors.initials: J
authors.lastname: Cavanagh
authors.forename: Rajiv
authors.initials: R
authors.lastname: Kumar
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D001665:Binding Sites; D064026:Calbindins; D003598:Cytoskeletal Proteins; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000378:metabolism*; D011233:Precipitin Tests; D017434:Protein Structure, Tertiary; D064030:S100 Calcium Binding Protein G / Q000737:chemistry* / Q000378:metabolism*; D020931:ran GTP-Binding Protein / Q000737:chemistry / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D064026:Calbindins; D003598:Cytoskeletal Proteins; D009687:Nuclear Proteins; C116005:Ran binding protein 9; D064030:S100 Calcium Binding Protein G; D020931:ran GTP-Binding Protein