Comparison of pencil-, fan-, and cone-beam dual X-ray absorptiometers for evaluation of bone mineral content in excised rat bone.
The aim of the present study was to assess the reproducibility and accuracy of measurements done on excised rat bone with three different generations of densitometers: Hologic QDR2000 pencil beam, Hologic QDR4500 fan beam, and Lunar PIXImus cone beam. The coefficients of variation for repeated measurements of bone mineral content (BMC) were 0.62 and 0.85% for pencil beam, 1.73 and 3.59% for fan beam, and 0.70 and 1.52% for cone beam for femur and tibia, respectively. BMC and ash weight were linearly correlated: 0.998 for pencil, 0.984 for fan, and 0.995 for cone beam. However, the three densitometers overestimated BMC by 10.9, 12.6, and 3.1%, respectively, and the overestimation was found to be dependent on the net BMC. The highest coefficient of correlation was found between BMC measurements from pencil and cone beam (r = 0.995). Data from cone-beam DXA were, respectively, 8.8 and 9.2% lower than those from penciland fan-beam DXA. We conclude that the three DXA instruments precisely and accurately measure BMC in excised rat bone; however, DXA overestimates BMC with a dependence on the bone ash weight. This dependence was less pronounced with the cone-beam technology.
Libouban
Hélène
H
LHEA-GEROM, Laboratoire d'Histologie-Embryologie, CHU & Faculté de Médecine, Angers Cédex, France.
Simon
Yannick
Y
Silve
Caroline
C
Legrand
Erick
E
Baslé
Michel Félix
MF
Audran
Maurice
M
Chappard
Daniel
D
D015502:Absorptiometry, Photon / Q000295:instrumentation* / Q000379:methods; D000818:Animals; D015519:Bone Density; D005269:Femur / Q000502:physiology; D008297:Male; D008903:Minerals; D051381:Rats; D013977:Tibia / Q000502:physiology
D008903:Minerals; C028705:bone ash