A study of the effect of surface damage on the uptake of Texas Red-BSA by schistosomula of Schistosoma mansoni.
In this paper we describe the effect of poly-L-lysines of different molecular weight on the schistosomula. In the control sample, the schistosomula of Schistosoma mansoni take up fluorescent Texas Red conjugated to bovine serum albumin (TxR-BSA) into the gut. Following slight damage by 24.0 kDa poly-L-lysine, a high proportion of schistosomula take up fluorescent TxR-BSA into the excretory system. Subsequently, the dye diffused into the bodies of the schistosomula. We suspected that this diffusion involved the process of endocytosis so we investigated this with the use of endocytosis inhibitor, Latrunculin A. Addition of the endocytosis inhibitor Latrunculin A following poly-L-lysine treatment inhibited gut uptake of TxR-BSA as well as the diffusion of excretory-ingested TxR-BSA molecules.
Tan
H H C
HH
Division of Biochemistry and Molecular Biology, The Davidson Building, Institute of Biomedical and Life Science, University of Glasgow, Glasgow G12 8QQ, UK.
Thornhill
J A
JA
Al-Adhami
B H
BH
Akhkha
A
A
Kusel
J R
JR
D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D004058:Diffusion; D004705:Endocytosis / Q000187:drug effects; D005456:Fluorescent Dyes / Q000378:metabolism*; D008970:Molecular Weight; D011107:Polylysine / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology*; D012550:Schistosoma mansoni / Q000187:drug effects* / Q000378:metabolism*; D012710:Serum Albumin, Bovine; D013844:Thiazoles / Q000494:pharmacology; D053778:Thiazolidines; D013997:Time Factors; D014966:Xanthenes / Q000378:metabolism*
D019086:Bridged Bicyclo Compounds, Heterocyclic; D005456:Fluorescent Dyes; D013844:Thiazoles; D053778:Thiazolidines; D014966:Xanthenes; D011107:Polylysine; D012710:Serum Albumin, Bovine; C034657:Texas red; C037067:latrunculin A