title: Associations among nucleated cell, CD34+ cell and colony-forming cell contents in cord blood units obtained through a standardized banking process.
abstract: OBJECTIVE
  Nucleated cell content is one of the main components used when evaluating cord blood (CB) units for clinical use. However, other indicators of the haematopoietic potential of a CB unit, such as CD34+ cell and colony-forming cell (CFU-TOT) content, have also been investigated. The aim of this study was to determine whether the CD34+ cell content could be used in selecting CB collections for banking.
  
  
  METHODS
  The collection data, as well as cellular contents of 588 CB collections obtained using a standardized CB banking process, were analysed.
  
  
  RESULTS
  Altogether, 526 CB units from the 588 collections accepted for processing were included in international search registries. The volume collected was, as expected, 69 ml (range 28-116 ml). The correlation between total CD34+ cell and CFU-TOT (n = 88) content in the CB collection was higher (r = 0.87) than the correlation between the total nucleated cell and CFU-TOT content (r = 0.69, both P < 0.0001). The correlations of pre- and postvolume reduction values of the total nucleated cell and CD34+ cell numbers were highly significant (r = 0.96, P < 0.0001, both). The total CFU-TOT content of the CB collection correlated significantly with the total CD34+ cell content of the CB unit before cryopreservation (but after volume reduction) (r = 0.89, P < 0.0001).
  
  
  CONCLUSIONS
  CD34+ cell content predicts the haematopoietic potential of a CB unit better than nucleated cell content. Accordingly, the CD34+ cell content of CB could be used to select CB for banking purposes and for transplantation.
authors.affiliation: Finnish Red Cross Blood Transfusion Service, Helsinki, Finland.
authors.forename: P
authors.initials: P
authors.lastname: Aroviita
authors.forename: K
authors.initials: K
authors.lastname: Teramo
authors.forename: P
authors.initials: P
authors.lastname: Westman
authors.forename: V
authors.initials: V
authors.lastname: Hiilesmaa
authors.forename: R
authors.initials: R
authors.lastname: Kekomäki
mesh_terms: D018952:Antigens, CD34 / Q000032:analysis*; D001771:Blood Banks / Q000592:standards; D001772:Blood Cell Count; D001800:Blood Specimen Collection; D005312:Fetal Blood / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007962:Leukocytes / Q000166:cytology; D012042:Registries; D012044:Regression Analysis; D000094902:Blood Banking / Q000379:methods*
chemical_list: D018952:Antigens, CD34