title: Sequence dependence of substrate recognition and cleavage by yeast RNase III.
abstract: Yeast Rnt1p is a member of the double-stranded RNA (dsRNA) specific RNase III family of endoribonucleases involved in RNA processing and RNA interference (RNAi). Unlike other RNase III enzymes, which recognize a variety of RNA duplexes, Rnt1p cleaves specifically RNA stems capped with the conserved AGNN tetraloop. This unusual substrate specificity challenges the established dogma for substrate selection by RNase III and questions the dsRNA contribution to recognition by Rnt1p. Here we show that the dsRNA sequence adjacent to the tetraloop regulates Rnt1p cleavage by interfering with RNA binding. In context, sequences surrounding the cleavage site directly influence the cleavage efficiency. Introduction of sequences that stabilize the RNA helix enhanced binding while reducing the turnover rate indicating that, unlike the tetraloop, Rnt1p binding to the dsRNA helix may become rate-limiting. These results suggest that Rnt1p activity is strictly regulated by a combination of primary and tertiary structural elements allowing a substrate-specific binding and cleavage efficiency.
authors.affiliation: Groupe ARN/RNA Group, Département de Microbiologie et d'Infectiologie, Faculté de Médecine, Université de Sherbrooke, 3001 12e Avenue Nord, J1H 5N4, Sherbrooke, Que., Canada.
authors.forename: Bruno
authors.initials: B
authors.lastname: Lamontagne
authors.forename: Ghada
authors.initials: G
authors.lastname: Ghazal
authors.forename: Isabelle
authors.initials: I
authors.lastname: Lebars
authors.forename: Satoko
authors.initials: S
authors.lastname: Yoshizawa
authors.forename: Dominique
authors.initials: D
authors.lastname: Fourmy
authors.forename: Sherif Abou
authors.initials: SA
authors.lastname: Elela
mesh_terms: D001483:Base Sequence; D002942:Circular Dichroism; D004722:Endoribonucleases / Q000378:metabolism*; D006868:Hydrolysis; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D009690:Nucleic Acid Conformation; D012330:RNA, Double-Stranded / Q000737:chemistry / Q000378:metabolism; D012331:RNA, Fungal / Q000737:chemistry / Q000378:metabolism; D043244:Ribonuclease III; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins; D013379:Substrate Specificity
chemical_list: D012330:RNA, Double-Stranded; D012331:RNA, Fungal; D029701:Saccharomyces cerevisiae Proteins; D004722:Endoribonucleases; C468367:RNT1 protein, S cerevisiae; D043244:Ribonuclease III