Specific tissue expression and cellular localization of human apolipoprotein M as determined by in situ hybridization.
Apolipoprotein M (apoM) is a recently discovered human apolipoprotein predominantly present in high-density lipoprotein (HDL), and in minor proportion in triglyceride-rich lipoprotein (TGRLP) and low-density lipoprotein (LDL). The gene coding for apoM has been detected in all mammal genomes. The function of apoM is unknown yet. In the present study, we demonstrated that apoM is exclusively expressed in a strong manner in adult liver and kidney, and is expressed weakly in fetal liver and kidney as detected with human multiple tissue expression array. Both immunohistochemical staining and apoM mRNA in situ hybridization demonstrated that apoM was exclusively expressed in hepatocytes in human liver and in tubular epithelial cells in human kidney. The present study helps to elucidate the pathophysiological functions of apoM in vivo.
Zhang
Xiao-Ying
XY
Laboratory of Molecular Medicine, The Third Affiliated Hospital, SuZhou University, ChangZhou, China.
Dong
Xuan
X
Zheng
Lu
L
Luo
Guang-Hua
GH
Liu
Yuan-Hua
YH
Ekström
Ulf
U
Nilsson-Ehle
Peter
P
Ye
Qing
Q
Xu
Ning
N
D001053:Apolipoproteins / Q000096:biosynthesis / Q000235:genetics*; D000075243:Apolipoproteins M; D005260:Female; D005333:Fetus; D019084:Fluorescent Antibody Technique, Indirect; D020869:Gene Expression Profiling; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D007668:Kidney / Q000378:metabolism; D054834:Lipocalins; D008099:Liver / Q000378:metabolism; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis
C400523:APOM protein, human; D001053:Apolipoproteins; D000075243:Apolipoproteins M; D054834:Lipocalins; D012333:RNA, Messenger