Influence of functional groups on the in vitro anticoagulant activity of chitosan sulfate.
A new method for the chemical modification of chitosan sulfate was used to prepare N-propanoyl-, N-hexanoyl- and N,O-quaternary substituted chitosan sulfate. Structural analysis by elemental analysis, FTIR, 13C NMR, and 1H NMR spectroscopy, and gel-permeation chromatography showed that these methods could conveniently be used for the introduction of functional groups. The influences of the acyl or quaternary groups on the anticoagulant activity of the polysaccharides were studied with respect to activated partial thromboplastin time (APTT) thrombin time (TT), and prothrombin time (PT). The propanoyl and hexanoyl groups increased the APTT activity, and the propanoyl groups also increased the TT anticoagulant activity slightly, while the N,O-quaternary chitosan sulfate showed only a slight TT coagulant activity.
Huang
Ronghua
R
Department of Environment Science, Wuhan University, Hubei, 430072, Wuhan, China.
Du
Yumin
Y
Yang
Jianhong
J
Fan
Lihong
L
D000215:Acylation; D000925:Anticoagulants / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects; D001780:Blood Coagulation Tests; D002686:Chitin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D048271:Chitosan; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D010314:Partial Thromboplastin Time; D011517:Prothrombin Time; D017550:Spectroscopy, Fourier Transform Infrared; D013997:Time Factors
D000925:Anticoagulants; C480355:chitosan sulfate; D002686:Chitin; C047203:N-hexanoylchitosan; D048271:Chitosan