title: Quantification of acetaldehyde released by lung cancer cells in vitro using selected ion flow tube mass spectrometry.
abstract: The production of volatile compounds from cancer cell lines in vitro has been investigated using selected ion flow tube mass spectrometry (SIFT-MS). This technique enables on-line quantitative analyses of the headspace above cell/medium cultures. This paper reports the discovery that acetaldehyde is released by the lung cancer cell lines SK-MES and CALU-1. The concentration of acetaldehyde in the headspace of the medium/cell culture was measured after 16 h incubation at 37 degrees C and found to be proportional to the number of cancer cells in the medium (typically 10(8)). From these data, the acetaldehyde production rates of the SK-MES cells and the CALU-1 cells in vitro are determined to be 1 x 10(6) and 1.5-3 x 10(6) molecules/cell/min, respectively. The potential value of this new technique in cell biology and in industrial cell biotechnology is discussed.
authors.affiliation: Centre for Science and Technology in Medicine, School of Postgraduate Medicine, Keele University, Thornburrow Drive, Hartshill, Stoke-on-Trent ST4 7QB, UK. d.smith@bemp.keele.ac.uk
authors.forename: David
authors.initials: D
authors.lastname: Smith
authors.forename: Tianshu
authors.initials: T
authors.lastname: Wang
authors.forename: Josep
authors.initials: J
authors.lastname: Sulé-Suso
authors.forename: Patrik
authors.initials: P
authors.lastname: Spanel
authors.forename: Alicia
authors.initials: A
authors.lastname: El Haj
mesh_terms: D000079:Acetaldehyde / Q000032:analysis* / Q000378:metabolism; D002452:Cell Count; D007477:Ions / Q000737:chemistry; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D014407:Tumor Cells, Cultured; D014835:Volatilization
chemical_list: D007477:Ions; D000079:Acetaldehyde