Inhibition of Streptomyces chromofuscus phospholipase D activity by dichloro-(2,2':6',2''-terpyridine)-platinum (II) dihydrate.
To determine the catalytic site of Streptomyces chromofuscus phospholipase D (PLD), which lacks an HKD motif, we examined the effects of inhibitors on the hydrolytic activity of the PLD by comparing it with cabbage and Streptomyces PLDs, which have two HKD motifs. We showed that dichloro-(2,2':6',2''-terpyridine)-platinum (II) dihydrate, a His- and Cys-directed chemical modifier, had inhibitory effects on the activities of all types of PLD examined. On the other hand, N-ethylmaleimide, a thiol-directed modifier had no such effects on PLD activity. These results suggest that the His residue plays an important role in the activity of Streptomyces chromofuscus PLD.
Kubota-Akizawa
Megumi
M
Research Institute for Biological Sciences, Okayama (RIBS), 7549-1 Kayo-cho, Jyobo-gun, Okayama 716-1241, Japan.
Negishi
Tomofumi
T
Mori
Koichi
K
Hatanaka
Tadashi
T
D017607:Aluminum Compounds / Q000494:pharmacology; D020816:Amino Acid Motifs; D001937:Brassica / Q000187:drug effects / Q000201:enzymology; D002118:Calcium / Q000378:metabolism; D017124:Conserved Sequence; D003545:Cysteine / Q000737:chemistry; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005033:Ethylmaleimide / Q000494:pharmacology; D005459:Fluorides / Q000494:pharmacology; D006639:Histidine / Q000737:chemistry; D006868:Hydrolysis / Q000187:drug effects; D007527:Isoenzymes; D010739:Phospholipase D / Q000037:antagonists & inhibitors*; D017671:Platinum Compounds / Q000737:chemistry / Q000494:pharmacology*; D013302:Streptomyces / Q000201:enzymology*; D013329:Structure-Activity Relationship
D017607:Aluminum Compounds; D004791:Enzyme Inhibitors; D007527:Isoenzymes; D017671:Platinum Compounds; D006639:Histidine; D010739:Phospholipase D; D003545:Cysteine; D005033:Ethylmaleimide; D005459:Fluorides; D002118:Calcium; C032311:aluminum fluoride