title: [Clinical and experimental studies on five cases of acute myeloid leukemia with translocation t(16;21)(p11;q22)].
abstract: OBJECTIVE
  To report five cases of acute myeloid leukemia (AML) with t(16;21)(p11;q22) translocation and the result of chromosome painting analysis on one of them.
  
  
  METHODS
  Chromosome specimens were prepared by short-term culture of bone marrow cells. Karyotype analysis was made by R-banding technique. Chromosome painting was performed using whole chromosome probes 16 and 21 in 1 case.
  
  
  RESULTS
  Karyotype analysis showed identical translocation t(16;21)(p11;q22) in all five cases, accounting for 0.3% of 1448 cases of acute myeoid leukemia examined in the past fifteen years. Moreover, chromosome painting distinctly demonstrated t(16;21) in one of them. Leukemia blasts did not show hemophagocytosis in all of them.
  
  
  CONCLUSIONS
  t(16;21) translocation is a rare and recurring chromosome rearrangement. It represents a specific type of AML. Chromosome painting technique is a more reliable means for detecting it, compared with the conventional karyotype analysis.
authors.affiliation: The First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology, Suzhou, Jiangsu, 215006 P. R. China. uujihsmc@public1.sz.js.cn
authors.forename: Yafang
authors.initials: Y
authors.lastname: Wu
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Xue
authors.forename: Jinlan
authors.initials: J
authors.lastname: Pan
authors.forename: Qinfeng
authors.initials: Q
authors.lastname: Ma
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D002478:Cells, Cultured; D002648:Child; D002885:Chromosomes, Human, Pair 16 / Q000235:genetics*; D002891:Chromosomes, Human, Pair 21 / Q000235:genetics*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D007951:Leukemia, Myeloid / Q000235:genetics*; D008297:Male; D014178:Translocation, Genetic