Inhibitory effect of Sejin-Eum I/II on nicotine- and cigarette extract-induced cytotoxicity in human lung fibroblast.
Nicotine is a major pharmacologically active component of cigarette smoke. Excessive cigarette smoking is harmful to lung. Sejin-Eum (SJE) I is composed of various Oriental medicines, and SJE II is SJE I plus seeds of Avena sativa (Gramineae) that reduces the craving for cigarette in man. In this study, we have examined whether an aqueous extract of SJE I/II inhibits nicotine- or cigarette extract (CE)-induced cytotoxicity in human embryonic lung fibroblast, MRC-9. Assessment of cell viability using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetric assay indicated that SJE I/II (500 and 1000 microg/ml) not only inhibited nicotine-induced cytotoxicity but also had significantly proliferous effect on MRC-9. However, SJE I/II had little effect on inhibition of CE-induced cytotoxicity. These results suggest the possibility that the use of SJE I/II may be useful for improvement of many symptoms by nicotine.
Jin
Jong-Sik
JS
College of Pharmacy, Wonkwang University, Iksan-city, Jeonbuk 570-749, South Korea.
Kim
Mi-Sun
MS
Yi
Jin-Mu
JM
Lee
Joon-Ho
JH
Lee
Jong-Hyun
JH
Moon
Su-Jin
SJ
Jung
Kyung-Phil
KP
Lee
Jai-Kyoo
JK
An
Nyeon-Hyoung
NH
Kim
Hyung-Min
HM
D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D008518:Medicine, East Asian Traditional; D009538:Nicotine / Q000037:antagonists & inhibitors*; D028321:Plant Preparations / Q000494:pharmacology*
D028321:Plant Preparations; D009538:Nicotine