Technical note: Specific PCR amplification of protozoal 18S rDNA sequences from DNA extracted from ruminal samples of cows.
A protozoa-specific primer (P-SSU-342f) was designed and paired with a eukarya-specific primer to amplify a 1,360-bp fragment of DNA encoding protozoal small subunit (SSU) ribosomal RNA from ruminal fluid of cows fed a mixed forage:grain diet or alfalfa hay. Sequencing of clones showed that P-SSU-342f is specific to ruminal protozoa and, with slight modifications, the primer will be useful for ecological studies of ruminal protozoa.
Karnati
S K R
SK
Department of Animal Sciences, The Ohio State University, Columbus 43210, USA.
Yu
Z
Z
Sylvester
J T
JT
Dehority
B A
BA
Morrison
M
M
Firkins
J L
JL
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D017931:DNA Primers; D016054:DNA, Protozoan / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D056890:Eukaryota / Q000145:classification* / Q000235:genetics; D005784:Gene Amplification; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012337:RNA, Ribosomal, 18S / Q000235:genetics*; D012417:Rumen / Q000469:parasitology*; D016415:Sequence Alignment / Q000662:veterinary
D017931:DNA Primers; D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S