[Preparation and application of monoclonal antibodies to recombinant human IFN alpha].
OBJECTIVE
To prepare and apply monoclonal antibodies (McAb) against recombinant human interferon alpha (rHu IFN-alpha).


METHODS
Five cell lines (2E9, 4G1, 2A7, 2C9, 4G10) secreting McAbs against rHu IFN-alpha were established by hybridoma technique.


RESULTS
All the cell lines secreted monoclonal antibodies stably. Functions of secreting antibodies of the five cell lines lasted for 6 months in BALB/c mice and 8 months in cell culture. The specificity of antibody was constant. The Ig subclasses of the McAbs were IgG1. Anti-IFN McAb affinity purification column was prepared by coupling the anti IFN-alpha McAb to Sepharose 4B. The combining rate reached was higher than 95%.


CONCLUSIONS
The highest purification efficiency was obtained by using 4G10 column.
Wu
Meiying
M
Beijing Tri-prime Genetic Engineering Co. Ltd, Beijing 102600, China.
Ai
Yanping
Y
Ren
Ren
R
Liang
Yixin
Y
Li
Jie
J
Song
Wenguang
W
Yang
Dajun
D
Sun
Jianbo
J
Cheng
Yongqing
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000915:Antibody Affinity; D000918:Antibody Specificity; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas / Q000378:metabolism; D007370:Interferon Type I / Q000276:immunology* / Q000302:isolation & purification; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins
D000911:Antibodies, Monoclonal; D007370:Interferon Type I; D011994:Recombinant Proteins