title: An immunohistological study of the localization of bacteria invading root pulpal walls of teeth with periapical lesions.
abstract: We immunohistologically examined the prevalence and localization of bacteria invading dentinal tubules of the roots of teeth with infected canals. Forty extracted teeth with apical lesions were selected and divided into two groups: a group of untreated teeth and a group of canal-enlarged teeth. The bacteria in the specimens were detected by Brown-Brenn stain and the labeled-streptavidin-biotin method with specific antisera for 16-bacteria. Seventy percent of the examined teeth showed bacteria invading the dentinal tubules of the roots. Fusobacterium nucleatum, Eubacterium alactolyticum, E. nodatum, Lactobacillus casei, and Peptostreptococcus micros were abundant. Even in the canal-enlarged group, invasion of bacteria was observed in 65% of teeth. This study revealed the actual condition of bacteria in infected root dentin and suggested that the canal-enlargement procedure could not completely remove all the bacteria in the infected dentinal tubules of the root.
authors.affiliation: Department of Conservative Dentistry, University of Tokushima School of Dentistry, Kuramoto, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Matsuo
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Shirakami
authors.forename: Kazumi
authors.initials: K
authors.lastname: Ozaki
authors.forename: Tadashi
authors.initials: T
authors.lastname: Nakanishi
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Yumoto
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Ebisu
mesh_terms: D000328:Adult; D000368:Aged; D001419:Bacteria / Q000145:classification / Q000648:ultrastructure*; D004396:Coloring Agents; D003782:Dental Pulp / Q000382:microbiology* / Q000648:ultrastructure; D003804:Dentin / Q000382:microbiology* / Q000648:ultrastructure; D005051:Eubacterium / Q000648:ultrastructure; D016967:Fusobacterium nucleatum / Q000648:ultrastructure; D006801:Humans; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D007780:Lacticaseibacillus casei / Q000648:ultrastructure; D008875:Middle Aged; D010462:Peptostreptococcus / Q000648:ultrastructure; D010483:Periapical Diseases / Q000382:microbiology* / Q000473:pathology; D017861:Porphyromonas / Q000648:ultrastructure; D018720:Prevotella / Q000648:ultrastructure; D018915:Root Canal Preparation; D018709:Statistics, Nonparametric; D014092:Tooth Root / Q000382:microbiology* / Q000648:ultrastructure
chemical_list: D004396:Coloring Agents; D007106:Immune Sera