title: Effect of two commercial vaccines to Campylobacter fetus subspecies on heifers naturally challenged.
abstract: Efficacy of two commercial vaccines containing Campylobacter fetus subspecies on heifers naturally challenged by service with an infected bull was tested. Sixteen heifers were vaccinated parentally two times with 3 weeks as interval, eight with commercial vaccine A and the other eight with commercial vaccine B. Eight other heifers were used as unvaccinated controls. Forty days after the first vaccine dose, the heifers were served by an infected bull during 60 days. Measure of systemic immune response and identification of the microorganism from genital secretions by culture and immunofluorescent antibody test (IFAT) were done. Vaccinated and control heifers had a poor reproductive performance (pregnancy rates were 2/8, 3/8 and 0/8 in groups A, B and C, respectively) and were infected by both methods during breeding time and after it. Moreover, one heifer in the groups B and C remained infected until 300 days post-breeding time. Neither vaccinated nor control heifers had an important increment of systemic antibody level. Only, they had a slight increment of antibody level after the breeding period and it may be because of natural stimulus by the infected bull during the copula. Culture and IFAT yielded high correlation on identification of C. fetus subspecies.
authors.affiliation: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
authors.forename: E R
authors.initials: ER
authors.lastname: Cobo
authors.forename: A
authors.initials: A
authors.lastname: Cipolla
authors.forename: C
authors.initials: C
authors.lastname: Morsella
authors.forename: D
authors.initials: D
authors.lastname: Cano
authors.forename: C
authors.initials: C
authors.lastname: Campero
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage; D015153:Blotting, Western / Q000662:veterinary; D002169:Campylobacter Infections / Q000517:prevention & control / Q000662:veterinary*; D002168:Campylobacter fetus / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D007115:Immunization Schedule; D007279:Injections, Subcutaneous / Q000662:veterinary; D008297:Male; D011247:Pregnancy; D018873:Pregnancy Rate
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines