[Inhibition of Luciferase expression in mammalian cells by AAV vector plasmid mediated Luciferase shRNA].
OBJECTIVE
Constructing a plasmid containing the shRNA of luciferase to suppress the expression of luciferase in BHK-21 cell.


METHODS
A 334 bp human U6 snRNA promoter was amplified from human genomic DNA by PCR and ligated to a 21 bp reverse repeated motif of luciferase target sequence with 9 bp spacer and AAV plasmid pSNAV. The recombinant pSNAV/U6/Luc plasmid cotransfected with pMAMneoLuc or transfected luciferase cell line to detect the effect of luciferase expression separately.


RESULTS
pSNAV/U6/luc suppresses the luciferase expression from pMAMneoLuc by 50% and luciferase cell line by 70%.


CONCLUSIONS
The results showed that the short hairpin RNA of luciferase can efficiently suppress its expression in BHK-21.
Ma
Xin
X
Institute of Virology,Chinese Academy of Preventive Medicine, Beijing 100052, China.
Lu
Xiaochun
X
Peng
Jainqiang
J
Tan
Shuping
S
Yuan
Zhenhua
Z
Yin
Fang
F
Wang
Hong
H
Wu
Xiaobing
X
Hou
Yunde
Y
D002460:Cell Line; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D008156:Luciferases / Q000096:biosynthesis* / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012313:RNA / Q000235:genetics*; D014162:Transfection
D012313:RNA; D008156:Luciferases