Factors secreted by human amniotic epithelial cells promote the survival of rat retinal ganglion cells.
We evaluated whether factors secreted by human amniotic epithelial cells (HAECs) have the neuroprotective effect on rat retinal ganglion cells (RGCs) purified by immunopanning. After culture in B27 complete medium containing B27 supplement, brain-derived neurotrophic factor (BDNF), ciliary neurotrophic factor (CNTF) and forskolin, the medium was changed to: (1). medium containing N2 supplement and forskolin (N2 basal medium); (2). medium conditioned by HAECs containing N2 supplement and forskolin (HAEC-CM); and (3). N2 basal medium containing several neurotrophic factors. HAEC-CM promoted the RGC survival compared to N2 basal medium. The effect of HAEC-CM was significantly higher than that of BDNF, neurotrophin-3 and CNTF. These results suggest that HAECs may produce unknown neuroprotective factors, suggesting its potential for the treatment of RGC degenerative diseases.
Uchida
Saiko
S
Department of Ophthalmology, Tokyo University School of Medicine, Tokyo 113-8655, Japan.
Suzuki
Yasuyuki
Y
Araie
Makoto
M
Kashiwagi
Kenji
K
Otori
Yasumasa
Y
Sakuragawa
Norio
N
D000650:Amnion / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D018696:Neuroprotective Agents / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012165:Retinal Ganglion Cells / Q000187:drug effects / Q000502:physiology*
D018696:Neuroprotective Agents