[Variation of mini- and microsatellite DNA repeats in parthenogenetic lizard Darevskia armeniaca as revealed by DNA fingerprinting analysis].
Population and family samples of two morphological forms (mutant and normal with respect to dorsal color) of pathogenetic lizard Darevskia armeniaca were examined by means of DNA fingerprinting using M13 mini- and (GATA)n and (TCC)n microsatellite DNA markers. The morphological forms examined were characterized by clonally inherited, species-specific patterns of the DNA markers, which were different from the species-specific DNA fingerprints of the other parthenogenetic species of the genus Darevskia (D. dahli. D. unisexualis, and D. rostombekovi). The mean index of similarity (S) obtained for a sample of 36 individuals from three isolated populations using three types of DNA markers was 0.966. This was similar to the variability level observed in D. dahli (0.962) (P > 0.05), but higher than that in D. unisexualis (0.950) (P < 0.05) and D. rostombekovi (0.875) (P < 0.01). Inheritance of M13 minisatellite and (TCC)n microsatellite DNA markers in the F1 offspring of parthenogenetic lizards was examined. It was shown that variability and clonal diversity of the fingerprint phenotypes observed in the populations and families of D. armeniaca could be at least partly explained by RFLP mutations in microsatellite repeats.
Martirosian
I A
IA
Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.
Kan
N G
NG
Petrosian
V G
VG
Malysheva
D N
DN
Trofimova
A A
AA
Danielian
F D
FD
Darevskiĭ
I S
IS
Korochkin
L I
LI
Ryskov
A P
AP
Tokarskaia
O N
ON
D000818:Animals; D003116:Color; D016172:DNA Fingerprinting; D014644:Genetic Variation; D005828:Genetics, Population; D008116:Lizards / Q000235:genetics* / Q000502:physiology; D018895:Microsatellite Repeats; D018598:Minisatellite Repeats; D009154:Mutation; D010312:Parthenogenesis / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D013045:Species Specificity