DC-SIGN (CD209) mediates dengue virus infection of human dendritic cells.
Dengue virus is a single-stranded, enveloped RNA virus that productively infects human dendritic cells (DCs) primarily at the immature stage of their differentiation. We now find that all four serotypes of dengue use DC-SIGN (CD209), a C-type lectin, to infect dendritic cells. THP-1 cells become susceptible to dengue infection after transfection of DC-specific ICAM-3 grabbing nonintegrin (DC-SIGN), or its homologue L-SIGN, whereas the infection of dendritic cells is blocked by anti-DC-SIGN antibodies and not by antibodies to other molecules on these cells. Viruses produced by dendritic cells are infectious for DC-SIGN- and L-SIGN-bearing THP-1 cells and other permissive cell lines. Therefore, DC-SIGN may be considered as a new target for designing therapies that block dengue infection.
Tassaneetrithep
Boonrat
B
Division of Retrovirology, Walter Reed Army Institite of Research and Henry M. Jackson Foundation for the Advancement of Military Medicine Diseases, Rockville, MD 20850, USA. mmarovich@hivresearch.org
Burgess
Timothy H
TH
Granelli-Piperno
Angela
A
Trumpfheller
Christine
C
Finke
Jennifer
J
Sun
Wellington
W
Eller
Michael A
MA
Pattanapanyasat
Kovit
K
Sarasombath
Suttipant
S
Birx
Deborah L
DL
Steinman
Ralph M
RM
Schlesinger
Sarah
S
Marovich
Mary A
MA
D000911:Antibodies, Monoclonal / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000276:immunology / Q000502:physiology*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000821:virology*; D003715:Dengue / Q000145:classification; D003716:Dengue Virus / Q000145:classification / Q000502:physiology*; D005434:Flow Cytometry; D006801:Humans; D037181:Lectins, C-Type / Q000276:immunology / Q000502:physiology*; D011956:Receptors, Cell Surface / Q000276:immunology / Q000502:physiology*; D011991:Receptors, Virus / Q000502:physiology; D012703:Serotyping; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D037181:Lectins, C-Type; D011956:Receptors, Cell Surface; D011991:Receptors, Virus; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin