Upregulation of mRNA encoding the M5 muscarinic acetylcholine receptor in human T- and B-lymphocytes during immunological responses.
Lymphocytes possess an independent, non-neuronal cholinergic system. Moreover, both T- and B-lymphocytes express multiple muscarinic acetylcholine receptors (mAChR). To obtain a better understanding of the regulatory mechanisms governing mAChR gene expression in the lymphocytic cholinergic system, we examined the effects of lymphocyte activation on expression of mAChR mRNA. Stimulation of T- and B-lymphocytes, respectively, with T-cell activator phytohemagglutinin and B-cell activator Staphylococcus aureus Cowan I upregulated M5 mAChR mRNA expression in the CEM human leukemic T-cell line and in the Daudi B-cell line, which served as models of lymphocytes. In striking contrast, M3 and M4 mAChR mRNA expression was not affected in either cell line. Nonetheless, stimulating lymphocytes with phorbol 12-myristate 13-acetate, a protein kinase C activator, plus ionomycin, a calcium ionophore, upregulated expression of both M3 and M5 mAChR mRNA. This represents the first demonstration that immunological stimulation leads to M5 mAChR gene expression in lymphocytes.
Fujii
Takeshi
T
Department of Pharmacology, Kyoritsu College of Pharmacy, 1-5-30 Shibakoen, Minato-ku, Tokyo 105-8512, Japan.
Watanabe
Yoshihiro
Y
Inoue
Tomoyuki
T
Kawashima
Koichiro
K
D000917:Antibody Formation / Q000502:physiology; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000494:pharmacology; D002352:Carrier Proteins / Q000494:pharmacology; D004338:Drug Combinations; D006801:Humans; D015759:Ionomycin / Q000494:pharmacology; D007476:Ionophores / Q000494:pharmacology; D010835:Phytohemagglutinins / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D043642:Receptor, Muscarinic M5; D011976:Receptors, Muscarinic / Q000235:genetics / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
D001426:Bacterial Proteins; D002352:Carrier Proteins; D004338:Drug Combinations; D007476:Ionophores; D010835:Phytohemagglutinins; D012333:RNA, Messenger; D043642:Receptor, Muscarinic M5; D011976:Receptors, Muscarinic; D015759:Ionomycin; D013755:Tetradecanoylphorbol Acetate