title: An efficient fusion expression system for protein and peptide overexpression in Escherichia coli and NMR sample preparation.
abstract: An efficient fusion expression system with a small fusion partner, His6-tagged N-terminal fragment of staphylococcal nuclease R, has been constructed and tested with two genes. The results show that the system is not only suitable for overexpression of small proteins and peptides but simplifies purification of target proteins and peptides. The study also provides a practical method for preparation of isotope-labeled protein sample for NMR analysis.
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Cheng
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Liu
authors.forename: Yanming
authors.initials: Y
authors.lastname: Feng
authors.forename: Guozhong
authors.initials: G
authors.lastname: Jing
mesh_terms: D003435:Crotalid Venoms / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics*; D009682:Magnetic Resonance Spectroscopy; D009363:Neoplasm Proteins / Q000235:genetics / Q000302:isolation & purification; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification
chemical_list: D003435:Crotalid Venoms; D009363:Neoplasm Proteins; D011993:Recombinant Fusion Proteins; C018932:myotoxin A