title: Development and validation of an automated extraction method (accelerated solvent extraction) and a reverse-phase HPLC analysis method for assay of ivermectin in a meat-based chewable formulation.
abstract: A new method for monitoring ivermectin content in HEARTGARD CHEWABLES has been developed and validated. The method consists of the automated extraction of ivermectin from the meat-based formulation under conditions of elevated temperature and pressure (accelerated solvent extraction, ASE, and determination of the active by reverse-phase high performance liquid chromatography (HPLC). The method resolves both active species of ivermectin (components H(2)B(1a) and H(2)B(1b)) from the formulation matrix.
authors.affiliation: Merck & Co, Inc, Merck Research Laboratories, Sumneytown Pike, Mail Stop WP 78-210, West Point, PA 9486, USA. andreas_abend@merck.com
authors.forename: Andreas M
authors.initials: AM
authors.lastname: Abend
authors.forename: Le
authors.initials: L
authors.lastname: Chung
authors.forename: David G
authors.initials: DG
authors.lastname: McCollum
authors.forename: W Peter
authors.initials: WP
authors.lastname: Wuelfing
mesh_terms: D000818:Animals; D002417:Cattle; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000639:trends; D007559:Ivermectin / Q000032:analysis* / Q000737:chemistry; D008460:Meat / Q000032:analysis*; D012997:Solvents / Q000032:analysis; D013678:Technology, Pharmaceutical / Q000379:methods* / Q000639:trends; D019155:Veterinary Drugs / Q000032:analysis / Q000737:chemistry
chemical_list: D012997:Solvents; D019155:Veterinary Drugs; D007559:Ivermectin