Application of electron spin resonance for evaluation of the level of free radicals in the myometrium in full-term pregnancy with normal labour and uterine inertia.
In order to identify and quantify free radicals in the tissues of patients with normal physiological and pathological states of births, we developed a method to evaluate the amount of free radicals in myometrium of subplacental area and from body of uterus, using electron spin resonance spectroscopy. Analysis of the concentration of free radicals in the myometrium in full-term pregnancy with normal labour and during uterine inertia was studied. The activities of Ca2+-ATPase, cytochrome c oxidase and succinate dehydrogenase in samples of these tissues were tested too. Low free radical concentrations in these tissues were associated with disturbances in contractile activity of myometrium along with reduction of Ca2+-ATPase, cytochrome c oxidase and succinate dehydrogenase activity. There proved to be an association between the level of free radicals in the tissues and alteration in the physiological processes.
Zyrianov
V V
VV
MMA Saratov Hospital N8, Bacteriological Laboratory, Lermontova Str., 1-15, Saratov, 410002, Russia. yrianov@san.ru
Sumovskaya
A Ye
AY
Shostak
A A
AA
D000252:Calcium-Transporting ATPases / Q000032:analysis / Q000378:metabolism; D036861:Delivery, Obstetric; D004578:Electron Spin Resonance Spectroscopy; D003576:Electron Transport Complex IV / Q000032:analysis / Q000378:metabolism; D005260:Female; D005609:Free Radicals / Q000032:analysis*; D006801:Humans; D009215:Myometrium / Q000378:metabolism*; D011247:Pregnancy; D013385:Succinate Dehydrogenase / Q000032:analysis / Q000378:metabolism; D014593:Uterine Inertia / Q000378:metabolism*
D005609:Free Radicals; D013385:Succinate Dehydrogenase; D003576:Electron Transport Complex IV; D000252:Calcium-Transporting ATPases