[Effects of burn serum on the viscoelasticity and the structure of rat intestinal epithelial cells].
OBJECTIVE
To investigate the effects of burn serum on the viscoelasticity and the structure of rat intestinal epithelial cells.


METHODS
The rat intestinal epithelial cell strain (IEC-6) was cultured and stimulated by burn serum. The changes of IECs before and after the stimulation were dynamically observed by cytoskeleton immunohistochemistry, ELISA and the measurement of cytomembranous viscoelasticity.


RESULTS
During the early stage of burn serum stimulation, the skeleton protein expression in IEC decreased obviously with weakened positive signals of microfilaments and microtubules and with decreased cellular elasticity.


CONCLUSIONS
The cytoskeleton injury could cause the increase of cellular fragility and the decrease of the viscoelasticity, which ultimately lead to the change of cellular biodynamics. These changes might directly participate the development of postburn intestinal epithelial injury.
Chen
Jun
J
Institute of Burn Research, Southwestern Hospital, The Third Military Medical University, Chongqing 400038 P.R. China.
Tang
Ao
A
Wu
Ze-Zhi
ZZ
Qin
Jian
J
Xiao
Guang-Xia
GX
D000199:Actins / Q000032:analysis; D000818:Animals; D002056:Burns / Q000097:blood*; D002460:Cell Line; D003470:Culture Media / Q000494:pharmacology; D003598:Cytoskeletal Proteins / Q000187:drug effects / Q000378:metabolism*; D004548:Elasticity / Q000187:drug effects; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014404:Tubulin / Q000032:analysis; D014783:Viscosity / Q000187:drug effects
D000199:Actins; D003470:Culture Media; D003598:Cytoskeletal Proteins; D014404:Tubulin