11C-harmine as a potential PET tracer for ductal pancreas cancer: in vitro studies.
Our objective was to find a tracer in diagnosing human pancreatic cancer using positron emission tomography (PET). For this purpose in vitro test of pancreatic tissues with autoradiography was used. Autoradiography was performed with (11)C-harmine (a MAO-A-inhibitor) with and without competitive inhibition. Tissue preparations were obtained from normal human pancreas and pancreatic cancer. The uptake was compared with rat brain or pig brain, tissues with high expression of MAO-A. Nine autoradiography studies on 16 samples from five different human pancreatic cancers gave a significant level of specific binding of (11)C-harmine in 13, and 3 samples did not give a significant level of specific binding of (11)C-harmine. All 16 samples were analysed with autoradiography. Compared with rat brain, the uptake in the human cancers varied between 9 and 43% except for one tissue preparation which had a too low value for measurement. This study shows expression of MAO-A in human pancreatic cancer. This is readily characterised in vitro. The potential use of (11)C-harmine in the diagnosis of pancreatic cancer using PET might be limited, but further PET studies are necessary.
Herlin
G
G
Department of Diagnostic Radiology, Huddinge University Hospital, 141-86 Stockholm, Sweden. Gunnar.Herlin@radiol.hs.sll.se
Persson
B
B
Bergström
M
M
Långström
B
B
Aspelin
P
P
D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000000981:diagnostic imaging; D002250:Carbon Radioisotopes; D006247:Harmine; D006801:Humans; D066298:In Vitro Techniques; D008995:Monoamine Oxidase / Q000378:metabolism; D010183:Pancreatic Ducts / Q000378:metabolism; D010190:Pancreatic Neoplasms / Q000000981:diagnostic imaging* / Q000378:metabolism; D051381:Rats; D013552:Swine; D014055:Tomography, Emission-Computed
D002250:Carbon Radioisotopes; D006247:Harmine; D008995:Monoamine Oxidase