A simple method for detecting up to five immunofluorescent parameters together with DNA staining for cell cycle or viability on a benchtop flow cytometer.
In this manuscript, we describe modifications to a commercial three-laser benchtop flow cytometer, as well as relevant biological methods, that allow analysis of up to five immunofluorescent parameters together with an ultraviolet (UV)-excitable DNA stain. This method allows expanded capacity for multiparameter immunophenotyping of complex mixed cell populations, together with accurate measurements of DNA content (cell cycle) or cell viability, on a stable, end-user operated platform.
Gordon
Kristie M
KM
Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
Duckett
Larry
L
Daul
Bruce
B
Petrie
Howard T
HT
D002453:Cell Cycle; D002470:Cell Survival; D004247:DNA / Q000032:analysis*; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D005455:Fluorescent Antibody Technique / Q000295:instrumentation / Q000379:methods*; D005456:Fluorescent Dyes; D007211:Indoles; D007834:Lasers; D011419:Propidium; D013194:Staining and Labeling / Q000379:methods
D005456:Fluorescent Dyes; D007211:Indoles; D011419:Propidium; C007293:DAPI; D004247:DNA