Growth inhibitory effect of triclosan on equine and bovine Babesia parasites.
We evaluated the growth inhibitory effect of triclosan, which has recently been reported to inhibit the growth of Plasmodium species and Toxoplasma gondii, on bovine and equine Babesia parasites in in vitro cultures The growth of Babesia bovis and B. bigemina was significantly inhibited in the presence of 100 microg/ml of triclosan, while B. caballi and B. equi were susceptible to as low as 50 microg/ml. Babesia bigemina and B. caballi were completely cleared as early as on the first and second day of the treatment, respectively. These parasites did not exhibit any growth in the subsequent five-day period of subculture without triclosan. Drug-treated parasites appeared pycnotic and atypically shaped, and ultrastructurally showed pronounced vacuolations, leading to complete destruction of parasites. Light microscopy showed that used concentrations of triclosan showed no toxicity against the host cells. The results suggest that triclosan can be used for chemotherapy of babesiosis.
Bork
Sabine
S
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan.
Yokoyama
Naoaki
N
Matsuo
Tomohide
T
Claveria
Florencia G
FG
Fujisaki
Kozo
K
Igarashi
Ikuo
I
D000818:Animals; D000981:Antiprotozoal Agents / Q000494:pharmacology*; D001403:Babesia / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D002417:Cattle / Q000469:parasitology*; D006736:Horses / Q000469:parasitology*; D008854:Microscopy, Electron; D014260:Triclosan / Q000494:pharmacology*
D000981:Antiprotozoal Agents; D014260:Triclosan