title: Differences in outcome of the interaction between Cryptococcus neoformans glucuronoxylomannan and human monocytes and neutrophils.
abstract: Disseminated infections by the opportunistic yeast Cryptococcus neoformans are characterized by accumulation in tissues of glucuronoxylomannan (GXM), the major component of the capsular polysaccharide. We investigated binding, uptake, and disposal of GXM by peripheral blood neutrophils and monocytes, and the effect of GXM uptake on phagocytic cell function. GXM was efficiently bound and internalized by both types of phagocytic cells, with maximal loading at 50 microg/ml, a GXM concentration found in serum and cerebrospinal fluid of some cryptococcosis patients. However, substantial differences were noted in the kinetics for uptake by macrophages and neutrophils. Whereas neutrophils rapidly ingested limited amounts of GXM and then expelled or degraded it after 1 h of incubation, macrophages demonstrated continuous intracellular accumulation for up to 1 week of incubation. Accumulation of GXM by neutrophils was accompanied by reduced anticryptococcal activity, suggesting one more mechanism for virulence enhancement by the major capsular component of C. neoformans.
authors.affiliation: Microbiology Section, Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Perugia, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Monari
authors.forename: Cinzia
authors.initials: C
authors.lastname: Retini
authors.forename: Arturo
authors.initials: A
authors.lastname: Casadevall
authors.forename: Dale
authors.initials: D
authors.lastname: Netski
authors.forename: Francesco
authors.initials: F
authors.lastname: Bistoni
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Kozel
authors.forename: Anna
authors.initials: A
authors.lastname: Vecchiarelli
mesh_terms: D000946:Antigens, Fungal / Q000378:metabolism*; D003455:Cryptococcus neoformans / Q000276:immunology* / Q000472:pathogenicity; D006801:Humans; D007700:Kinetics; D009000:Monocytes / Q000276:immunology*; D009504:Neutrophils / Q000166:cytology / Q000276:immunology*; D010587:Phagocytosis; D011134:Polysaccharides / Q000276:immunology / Q000378:metabolism*; D013696:Temperature
chemical_list: D000946:Antigens, Fungal; D011134:Polysaccharides; C027478:glucuronoxylomannan