Differentiation of cones in cultured rabbit retina: effects of retinal pigment epithelial cell-conditioned medium.
This study was aimed at investigating the postnatal differentiation of cone photoreceptors in the rabbit retina in an organotypic explant culture system. Both short wavelength (S) and middle wavelength (M) cone opsins were expressed in culture but M cones appeared only in retinal explants from the dorsal half of the eye. Stimulating the explants with retinal pigment epithelial cell (RPE) conditioned medium resulted in a suppression of opsin expression despite of an increase of the number of presumptive peanut agglutinin-labeled cones. These results suggest that at birth the immature cones are largely undetermined in terms of their final cone identity although some positional information ('dorsal' vs. 'ventral' retina) is present. Furthermore, factors from RPE may inhibit as well as stimulate different steps of cone cell differentiation.
Mack
Andreas F
AF
Paul-Flechsig-Institut für Hirnforschung, Universität Leipzig, Jahnallee 59, D-04109 Leipzig, Germany.
Uhlmann
Daniela
D
Germer
Angela
A
Szél
Agoston
A
Enzmann
Volker
V
Reichenbach
Andreas
A
D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000502:physiology*; D017077:Culture Media, Conditioned / Q000494:pharmacology; D009924:Organ Culture Techniques / Q000379:methods; D010857:Pigment Epithelium of Eye / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D011817:Rabbits; D012160:Retina / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism
D017077:Culture Media, Conditioned