title: DNA restriction is a barrier to natural transformation in Pseudomonas stutzeri JM300.
abstract: Natural transformation is a mechanism for intra- and interspecific transfer of chromosomal DNA in Pseudomonas stutzeri. During this process a single strand derived from duplex DNA is transported into the cytoplasm and recombined with resident DNA. By electroporation, which introduces duplex DNA into cells, 100-fold lower transformation frequencies of P. stutzeri JM300 were observed with shuttle vector or broad-host-range plasmid DNA when the plasmids had replicated in Escherichia coli and not in P. stutzeri JM300. Moreover, the natural transformation with cloned chromosomal P. stutzeri JM300 DNA was reduced about 40-fold when the DNA had not been propagated in P. stutzeri JM300 but in E. coli. Restriction was also active during natural transformation by single-stranded DNA. Restriction during natural transformation and electroporation was abolished in mutants isolated from mutagenized JM300 cells after applying a multiple plasmid electroporation strategy for the enrichment of restriction-defective strains. The mutants had retained the ability for DNA modification. The P. stutzeri strain ATCC 17587 was found to have no restriction-modification system as seen in JM300. It is discussed whether restriction during natural transformation acts at presynaptic or postsynaptic stages of transforming DNA. Restriction as a barrier to transformation apparently contributes to sexual isolation and therefore may promote speciation in the highly diverse species P. stutzeri.
authors.affiliation: Genetik, Fachbereich Biologie, Geo- und Umweltwissenschaften, Carl von Ossietzky Universität Oldenburg, POB 2503, D-26111 Oldenburg, Germany.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Berndt
authors.affiliation: Genetik, Fachbereich Biologie, Geo- und Umweltwissenschaften, Carl von Ossietzky Universität Oldenburg, POB 2503, D-26111 Oldenburg, Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: Meier
authors.affiliation: Genetik, Fachbereich Biologie, Geo- und Umweltwissenschaften, Carl von Ossietzky Universität Oldenburg, POB 2503, D-26111 Oldenburg, Germany.
authors.forename: Wilfried
authors.initials: W
authors.lastname: Wackernagel
mesh_terms: D015280:DNA Restriction-Modification Enzymes / Q000235:genetics / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000378:metabolism; D018274:Electroporation; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D006801:Humans; D009154:Mutation; D010957:Plasmids; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics*; D011995:Recombination, Genetic; D015183:Restriction Mapping; D014169:Transformation, Bacterial
chemical_list: D015280:DNA Restriction-Modification Enzymes; D004269:DNA, Bacterial; D004277:DNA, Single-Stranded