Characterization and crystallization of active domains of a novel luciferase from a marine dinoflagellate.
Lingulodinium polyedrum luciferase is a bioluminescent protein found in the marine dinoflagellate formerly known as Gonyaulax. It is located in organelles called scintillons that emit brief and bright flashes of light that are regulated by an endogenous circadian clock. The complete luciferase molecule has a molecular mass of 136 994 Da and contains three homologous domains, each of which is a separately active luciferase. Two of these domains, D2-LCF and D3-LCF, have been cloned, expressed and crystallized. Crystals of D2-LCF were obtained from PEG 10 000 in space group P2(1)2(1)2(1), with unit-cell parameters a = 49.1, b = 104.7, c = 180.3 A. They diffract to 2.9 A on a rotating anode. Crystals of D3-LCF were grown from PEG 2000 in space group P2(1)2(1)2(1), with unit-cell parameters a = 58.86, b = 63.98, c = 95.76 A. They diffract to 2.3 A on a rotating anode.
Liu
Liyun
L
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138-2020, USA.
Im
Hookang
H
Cegielski
Margaret
M
LeMagueres
Pierre
P
Schultz
L Wayne
LW
Krause
Kurt L
KL
Hastings
J Woodland
JW
D000818:Animals; D001665:Binding Sites; D003001:Cloning, Molecular; D003460:Crystallization; D004141:Dinoflagellida / Q000201:enzymology* / Q000235:genetics / Q000648:ultrastructure; D015870:Gene Expression; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008156:Luciferases / Q000737:chemistry* / Q000235:genetics; D012645:Selenomethionine / Q000737:chemistry; D014961:X-Ray Diffraction
D007202:Indicators and Reagents; D012645:Selenomethionine; D008156:Luciferases