Genetic instability in superficial bladder cancer and adjacent mucosa: an interphase cytogenetic study.
A systematic analysis of both tumors and the surrounding urothelium to help identify what lies behind the mechanism of multifocal tumor development has not yet been performed. In this study we investigated chromosome 1, 7, 9, and 17 aneusomy in 25 superficial papillary carcinomas and in 51 tissue samples taken from sites of macroscopically uninvolved urothelium surrounding the tumors, using the fluorescence in situ hybridization method. Our data demonstrated a close genetic relationship between all examined tumors and normal-appearing mucosa. Numeric aberrations of chromosomes 1, 7, 9, and 17 were found to exhibit similar patterns in all analyzed specimens, although with different frequencies.
Cianciulli
Anna Maria
AM
Department of Clinical Pathology, Regina Elena Cancer Institute, Rome, Italy.
Leonardo
Costantino
C
Guadagni
Fiorella
F
Marzano
Raffaella
R
Iori
Francesco
F
De Nunzio
Cosimo
C
Franco
Giorgio
G
Merola
Roberta
R
Laurenti
Cesare
C
D002291:Carcinoma, Papillary / Q000235:genetics*; D002869:Chromosome Aberrations; D002878:Chromosomes, Human, Pair 1; D002886:Chromosomes, Human, Pair 17; D002897:Chromosomes, Human, Pair 7; D002899:Chromosomes, Human, Pair 9; D020732:Cytogenetic Analysis; D005456:Fluorescent Dyes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007211:Indoles; D009092:Mucous Membrane / Q000648:ultrastructure; D001749:Urinary Bladder Neoplasms / Q000235:genetics*; D019459:Urothelium / Q000648:ultrastructure
D005456:Fluorescent Dyes; D007211:Indoles; C007293:DAPI