Mutation analysis of IgVH gene in B-cell chronic lymphocytic leukemia.
The variable heavy chain region (VH) genes of 3 untreated patients with B-cell chronic lymphocytic leukemia (B-CLL) were cloned and analyzed. The VH family used was VH3-11, VH3-72 and VH3-33. More than 2% difference from the corresponding germline gene was detected in all the 3 obtained potential functional genes (average 16.7). Mutation pattern analysis indicated evidence of antigen selective pressure observed in 1 of 3 cases. Our findings suggested that the tumor cells originate from post-GC cells.
Wang
Feng
F
Institute of Immunology, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
Zhu
Huifen
H
Zhu
Lijuan
L
Yin
Botao
B
Shen
Guanxin
G
D000595:Amino Acid Sequence; D015321:Gene Rearrangement; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation
D007135:Immunoglobulin Variable Region