Identification of chromosome rearrangements between the laboratory mouse (Mus musculus) and the Indian spiny mouse (Mus platythrix) by comparative FISH analysis.
Comparative chromosome painting was applied to the Indian spiny mouse (Mus platythrix) with mouse (M. musculus) chromosome-specific probes for understanding the process of chromosome rearrangements between the two species. The chromosome locations of the 5S and 18S-28S ribosomal RNA genes and the order of the 119 and Tcp-1 genes in the In(17)2 region of the t-complex were also compared. All the painting probes were successfully hybridized to the Indian spiny mouse chromosomes, and a total of 27 segments homologous to mouse chromosomes were identified. The comparative FISH analysis revealed that tandem fusions were major events in the chromosome evolution of the Indian spiny mouse. In addition, other types of chromosome rearrangements, i.e. reciprocal translocations and insertions, were also included.
Matsubara
Kazumi
K
Laboratory of Cytogenetics, Division of Bioscience, Graduate School of Environmental Earth Science, Hokkaido University, North 10 West 8, Kita-ku, Sapporo 060-0810, Japan.
Nishida-Umehara
Chizuko
C
Kuroiwa
Asato
A
Tsuchiya
Kimiyuki
K
Matsuda
Yoichi
Y
D000818:Animals; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002875:Chromosomes; D003582:Cytogenetics; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D012341:RNA, Ribosomal, 5S / Q000235:genetics
D012337:RNA, Ribosomal, 18S; D012339:RNA, Ribosomal, 28S; D012341:RNA, Ribosomal, 5S