Overexpression of the peroxisome proliferator activated receptor alpha or the human c-Ha-ras transgene is not involved in tumorigenesis induced by di(2-ethylhexyl)phthalate in rasH2 mice.
The mRNA profiles for peroxisome proliferator activated receptor (PPAR) alpha and human c-Ha-ras genes were determined by real-time semi-quantitative polymerase chain reaction analysis of hepatocellular adenomas induced by di(2-ethylhexyl)phthalate (DEHP) in transgenic mice carrying a human prototype c-Ha-ras gene (rasH2 mice). The mRNA levels were essentially equal in hepatocellular adenomas and adjacent non-neoplastic hepatocytes, in spite of a remarkable elevation in the cell proliferation index in tumors determined by anti-Ki-67 immunohistochemistry. From the results, it is concluded that overexpression of PPARalpha or the transgene is not associated with the liver tumorigenesis induced by DEHP in rasH2 mice.
Toyosawa
Kaoru
K
Drug Research Division, Dainippon Pharmaceutical Co., Ltd., 33-94 Enoki, Suita, 564-0053, Osaka, Japan. kaoru-toyozawa@dianippon-pharm.co.jp
Okugawa
Kenji
K
Teranishi
Yasuhiro
Y
Tanaka
Kohji
K
Matsuoka
Nobuo
N
D018248:Adenoma, Liver Cell / Q000139:chemically induced* / Q000235:genetics* / Q000473:pathology; D000818:Animals; D002455:Cell Division; D004051:Diethylhexyl Phthalate / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors; D004051:Diethylhexyl Phthalate