Random amplified polymorphic DNA analysis of DNA extracted from Trichuris trichiura (Linnaeus, 1771) eggs and its prospective application to paleoparasitological studies.
Random amplified polymorphic DNA analysis was applied to DNAs extracted from Trichuris trichiura eggs recovered from human fecal samples. Four out of 6 primers tested displayed 18 distinct and well defined polymorphic patterns, ranging from 650 to 3200 base pairs. These results, upon retrieval and DNA sequencing of some of these bands from agarose gels, might help in establishing. T. trichiura specific genetic markers, not available yet, and an important step to design primers to be used in molecular diagnosis approaches.
Martinez
Elaine Machado
EM
Laboratório de Paleoparasitologia Molecular Eduardo Marques, Departamento de Endemias Samuel Pessoa, Escola Nacional de Saúde Pública-Fiocruz, Rio de Janeiro, RJ, Brasil.
Correia
Jorge Antonio Santos
JA
Villela
Erika Verissimo
EV
Duarte
Antonio Nascimento
AN
Ferreira
Luiz Fernando
LF
Bello
Alexandre Ribeiro
AR
D000818:Animals; D001483:Base Sequence; D018509:DNA, Helminth / Q000235:genetics / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D005819:Genetic Markers; D006801:Humans; D008969:Molecular Sequence Data; D010063:Ovum; D019105:Random Amplified Polymorphic DNA Technique; D014258:Trichuris / Q000235:genetics*
D018509:DNA, Helminth; D005819:Genetic Markers