Golgin-84 is a rab1 binding partner involved in Golgi structure.
Members of the golgin family of coiled-coil proteins have been implicated in the tethering of vesicles to Golgi membranes and cisternal membranes to each other. Many also bind to rab GTPases. Golgin-84 is a membrane-anchored golgin that we now show binds preferentially to the GTP form of the rab1 GTPase. It is also present throughout the Golgi stack by immuno-EM. Antibodies to golgin-84 inhibit stacking of cisternal membranes in a cell-free assay for Golgi reassembly, whereas the cytoplasmic domain of golgin-84 stimulates stacking and increases the length of re-assembled stacks. Transient expression of golgin-84 in NRK cells helps prevent the disassembly of the Golgi apparatus normally triggered by treatment with brefeldin A. Together these data suggest that golgin-84 is involved in generating and maintaining the architecture of the Golgi apparatus.
Satoh
Ayano
A
Department of Cell Biology, Yale University School of Medicine, 333 Cedar Street, PO Box 208002, New Haven, Connecticut 06520-8002, USA.
Wang
Yanzhuang
Y
Malsam
Jörg
J
Beard
Matthew B
MB
Warren
Graham
G
D000818:Animals; D002478:Cells, Cultured; D020285:Cryoelectron Microscopy; D006056:Golgi Apparatus / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D008099:Liver / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D008565:Membrane Proteins / Q000378:metabolism*; D016253:Microscopy, Immunoelectron; D008938:Mitosis; D008969:Molecular Sequence Data; D011485:Protein Binding; D051381:Rats; D020761:rab1 GTP-Binding Proteins / Q000378:metabolism*
D008565:Membrane Proteins; D020761:rab1 GTP-Binding Proteins