Changes in enantiomeric fractions during microbial reductive dechlorination of PCB132, PCB149, and araclor 1254 in Lake Hartwell sediment microcosms.
The enantioselectivity of microbial reductive dechlorination of chiral PCBs in sediments from Lake Hartwell, SC, was determined by microcosm studies and enantiomer-specific GC analysis. Sediments from two locations in the vicinity of the highest levels of PCB contamination were used as inocula. Dechlorination activity was monitored by concentration decreases in the spiked chiral PCBs and formation of dechlorination products using both achiral and chiral chromatography. Live microcosms spiked with PCB132 (234-236) exhibited dechlorination of PCB132 to PCB91 (236-24) and PCB51 (24-26). Meta dechlorination was the dominant mechanism. Microcosms spiked with PCB149 (245-236) exhibited preferential para dechlorination of PCB149 to PCB95 (236-25), followed by meta dechlorination to PCB53 (25-26) and subsequently PCB19 (26-2). Dechlorination of chiral PCB132 and PCB149 was not enantioselective. In Aroclor 1254-spiked microcosms, reductive dechlorination of PCB149 also was nonenantioselective. These results suggest that dechlorinating enzymes responsible for the dehalogenation of the chiral PCB132 and PCB149 congeners bind the two enantiomers equally. Reductive dechlorination of PCB91 and PCB95, however, occurred in an enantioselective manner, indicating that the dechlorinating enzymes for these PCBs are enantiomer-specific. The chlorine substitution pattern on the biphenyl ring appears to influence whether reductive dechlorination of chiral PCB congeners is enantioselective. Enantioselective PCB dechlorination by the microbial population of Lake Hartwell sediments occurs for select chiral PCBs; thus, certain chiral PCBs might be useful as markers for in situ reductive dechlorination.
Pakdeesusuk
Usarat
U
Department of Environmental Engineering & Science, Clemson University, Clemson, South Carolina 29634-0919, USA.
Jones
W Jack
WJ
Lee
Cindy M
CM
Garrison
Arthur W
AW
O'Niell
Walter L
WL
Freedman
David L
DL
Coates
John T
JT
Wong
Charles S
CS
D013956:Antithyroid Agents / Q000378:metabolism*; D020111:Chlorodiphenyl (54% Chlorine) / Q000378:metabolism*; D002849:Chromatography, Gas; D004784:Environmental Monitoring; D004785:Environmental Pollutants / Q000378:metabolism*; D019015:Geologic Sediments / Q000737:chemistry*; D011078:Polychlorinated Biphenyls / Q000378:metabolism*; D013022:South Carolina; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000378:metabolism*
D013956:Antithyroid Agents; D004785:Environmental Pollutants; D014874:Water Pollutants, Chemical; D020111:Chlorodiphenyl (54% Chlorine); C094859:2,2',3,3',4,6'-hexachlorobiphenyl; D011078:Polychlorinated Biphenyls