Enhanced nutritionally induced adipose tissue development in mice with stromelysin-1 gene inactivation.
To investigate a potential role of stromelysin-1 (MMP-3) in development of adipose tissue, 5 week old male MMP-3 deficient mice (MMP-3(-/-)) and wild-type (MMP-3(+/+)) controls were kept on a high fat diet (HFD) for 15 weeks. MMP-3(-/-) mice were hyperphagic and gained more weight than the MMP-3(+/+) mice. At the time of sacrifice, the body weight of the MMP-3(-/-) mice was significantly higher than that of the MMP-3(+/+) mice, as was the weight of the isolated subcutaneous (SC) and gonadal (GON) fat deposits. Significant adipocyte hypertrophy was observed in the GON but not in the SC adipose tissue of MMP-3(-/-) mice. Fasting plasma glucose and cholesterol levels were comparable in both genotypes, whereas triglyceride levels were significantly lower in MMP-3(-/-) mice. Staining with an endothelial cell specific lectin revealed a significantly higher blood vessel density and larger total stained area in the GON adipose tissues of MMP-3(-/-) mice. Thus, in a murine model of nutritionally induced obesity, MMP-3 impairs adipose tissue development, possibly by affecting food intake and/or adipose tissue-related angiogenesis.
Maquoi
Erik
E
Center for Molecular and Vascular Biology, University of Leuven, Campus Gasthuisberg, O & N, Herestraat 49, B-3000 Leuven, Belgium.
Demeulemeester
Diego
D
Vörös
Gabor
G
Collen
Désire
D
Lijnen
H Roger
HR
D017667:Adipocytes / Q000378:metabolism; D000273:Adipose Tissue / Q000378:metabolism*; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001786:Blood Glucose; D015153:Blotting, Western; D001835:Body Weight; D002784:Cholesterol / Q000097:blood; D004247:DNA / Q000378:metabolism; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005838:Genotype; D007150:Immunohistochemistry; D020738:Leptin / Q000096:biosynthesis; D008297:Male; D019278:Matrix Metalloproteinase 3 / Q000235:genetics* / Q000502:physiology*; D051379:Mice; D018919:Neovascularization, Physiologic; D009765:Obesity / Q000209:etiology; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014280:Triglycerides / Q000378:metabolism
D001786:Blood Glucose; D020738:Leptin; D012333:RNA, Messenger; D014280:Triglycerides; D004247:DNA; D002784:Cholesterol; D019278:Matrix Metalloproteinase 3