title: Loss of HSulf-1 up-regulates heparin-binding growth factor signaling in cancer.
abstract: Emerging data suggest that signaling by heparin-binding growth factors is influenced by the sulfation state of N-acetylglucosamine residues of heparan sulfate proteoglycans (HSPGs). Here we report that the recently identified protein HSulf-1, a heparin-degrading endosulfatase, encodes a cell surface-associated enzyme that diminishes sulfation of cell surface HSPGs. The message encoding this enzyme is readily detectable in a variety of normal tissues, including normal ovarian surface epithelial cells, but is undetectable in 5 of 7 ovarian carcinoma cell lines and markedly diminished or undetectable in approximately 75% of ovarian cancers. Similar down-regulation is also observed in breast, pancreatic, renal cells, and hepatocellular carcinoma lines. Re-expression of HSulf-1 in ovarian cancer cell lines resulted in diminished HSPG sulfation, diminished phosphorylation of receptor tyrosine kinases that require sulfated HSPGs as co-receptors for their cognate ligands, and diminished downstream signaling through the extracellular signal-regulated kinase pathway after treatment with fibroblast growth factor-2 or heparin-binding epidermal growth factor. Consistent with these changes, HSulf-1 re-expression resulted in reduced proliferation as well as sensitivity to induction of apoptosis by the broad spectrum kinase inhibitor staurosporine and the chemotherapeutic agent cisplatin. Collectively, these observations provide evidence that HSulf-1 modulates signaling by heparin-binding growth factors, and HSulf-1 down-regulation represents a novel mechanism by which cancer cells can enhance growth factor signaling.
authors.affiliation: Mayo Clinic Cancer Center, Mayo Clinic and Foundation, Rochester, Minnesota 55905, USA.
authors.forename: Jinping
authors.initials: J
authors.lastname: Lai
authors.forename: Jeremy
authors.initials: J
authors.lastname: Chien
authors.forename: Julie
authors.initials: J
authors.lastname: Staub
authors.forename: Rajeswari
authors.initials: R
authors.lastname: Avula
authors.forename: Eddie L
authors.initials: EL
authors.lastname: Greene
authors.forename: Tori A
authors.initials: TA
authors.lastname: Matthews
authors.forename: David I
authors.initials: DI
authors.lastname: Smith
authors.forename: Scott H
authors.initials: SH
authors.lastname: Kaufmann
authors.forename: Lewis R
authors.initials: LR
authors.lastname: Roberts
authors.forename: Viji
authors.initials: V
authors.lastname: Shridhar
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics; D001943:Breast Neoplasms; D006528:Carcinoma, Hepatocellular; D002455:Cell Division; D003001:Cloning, Molecular; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004847:Epithelial Cells; D005260:Female; D016220:Fibroblast Growth Factor 1 / Q000494:pharmacology*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic; D066257:Heparin-binding EGF-like Growth Factor; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D008113:Liver Neoplasms; D010051:Ovarian Neoplasms; D010053:Ovary; D011994:Recombinant Proteins / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D015238:Sulfotransferases / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D020121:5' Untranslated Regions; C587883:HBEGF protein, human; D066257:Heparin-binding EGF-like Growth Factor; D036341:Intercellular Signaling Peptides and Proteins; D011994:Recombinant Proteins; D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1; D004815:Epidermal Growth Factor; C470791:SULF1 protein, human; D015238:Sulfotransferases