title: High-performance liquid chromatographic method with diode array detection to identify and quantify atypical antipsychotics and haloperidol in plasma after overdose.
abstract: For toxicological purposes, an HPLC assay was developed for the simultaneous determination of haloperidol and atypical antipsychotics (risperidone, 9-hydroxyrisperidone, olanzapine, clozapine) in human plasma. After a double-step liquid-liquid extraction, compounds were separated on a C(8) column eluted with a gradient of acetonitrile and phosphate buffer 50 mM pH 3.8. A sequential ultraviolet detection was used (260, 280 and 240 nm). Calibration curves were linear in the range 10-1000 ng/ml. The limits of quantification were 5 ng/ml for all drugs. Average accuracy at four concentrations ranged from 93 to 109%. Both inter- and intra-day variation coefficients were lower than 11% for all drugs. This simple and rapid method (run time<15 min) is currently used for poison management.
authors.affiliation: Department of Clinical Pharmacology and Toxicology, Pellegrin Hospital and University Victor Segalen, Place Amélie Raba-Léon, 33076 Bordeaux, France. karine.titier@pharmaco.u-bordeaux2.fr
authors.forename: K
authors.initials: K
authors.lastname: Titier
authors.forename: S
authors.initials: S
authors.lastname: Bouchet
authors.forename: F
authors.initials: F
authors.lastname: Péhourcq
authors.forename: N
authors.initials: N
authors.lastname: Moore
authors.forename: M
authors.initials: M
authors.lastname: Molimard
mesh_terms: D014150:Antipsychotic Agents / Q000097:blood* / Q000506:poisoning; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D062787:Drug Overdose / Q000097:blood*; D006220:Haloperidol / Q000097:blood* / Q000506:poisoning; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D014150:Antipsychotic Agents; D006220:Haloperidol