title: Delayed phosphorylation of p38 mitogen-activated protein kinase in the AT1a knock-out mouse striatal neurons during middle cerebral artery occlusion and reperfusion.
abstract: To investigate whether the phosphorylation of p38 in cerebral ischemia occurs via angiotensin II receptor type 1a (AT1a), we examined the time course of phosphorylation of p38 and proline-rich tyrosine kinase 2 in AT1a knock-out mouse striatal neurons during middle cerebral artery occlusion (MCAO) and reperfusion. Phosphorylated-p38 was observed after 2 h and 5 h of reperfusion after 1 h of MCAO in C57/B6 mice and AT1a knock out mice, respectively. We demonstrated a delay of phosphorylation of p38 in the reperfusion model of the AT1a knock-out mouse, and detected microglia in the striatum on the ischemic side that were phosphorylated-p38-positive after 71 h of reperfusion in both animals. However, there was no association between AT1a and delayed neuronal cell death, or between AT1a and activation of caspase-9 in cerebral ischemia/reperfusion.
authors.affiliation: Department of Neurology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan. hhattori@sc.itc.keio.ac.jp
authors.forename: Hidenori
authors.initials: H
authors.lastname: Hattori
authors.forename: Mamoru
authors.initials: M
authors.lastname: Shibata
authors.forename: Takeshi
authors.initials: T
authors.lastname: Sugaya
authors.forename: Junichi
authors.initials: J
authors.lastname: Hamada
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Fukuuchi
mesh_terms: D000818:Animals; D003342:Corpus Striatum / Q000201:enzymology* / Q000473:pathology; D020244:Infarction, Middle Cerebral Artery / Q000201:enzymology* / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D009474:Neurons / Q000201:enzymology* / Q000473:pathology; D010766:Phosphorylation; D044140:Receptor, Angiotensin, Type 1; D011945:Receptors, Angiotensin / Q000172:deficiency* / Q000235:genetics; D015427:Reperfusion Injury / Q000201:enzymology* / Q000473:pathology; D048051:p38 Mitogen-Activated Protein Kinases
chemical_list: D044140:Receptor, Angiotensin, Type 1; D011945:Receptors, Angiotensin; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases