Gaf-1b is an alternative splice variant of Gaf-1/Rip11.
Gaf-1/Rip11 encoded by the clone KIAA0857 participates in endosomal recycling through the interaction with both gamma-SNAP, a member of the soluble NSF attachment protein family, and a small GTPase, Rab11. Gaf-1/Rip11 and other Rab11-interacting proteins constitute a novel protein family that is involved in the endocytic pathways. Here we report the presence of an alternative splice variant of Gaf-1/Rip11 named Gaf-1b. Gaf-1b also interacts with gamma-SNAP and is expressed ubiquitously in tissues except for liver. Subcellular fractionation analysis revealed that Gaf-1b, as well as Gaf-1/Rip11, is mainly present in the microsomal fraction. Overexpression of Gaf-1b, like that of Gaf-1/Rip11, affected the morphology of recycling endosomes. These results suggest that Gaf-1b has a similar function to Gaf-1/Rip11.
Kawase
Kazuho
K
School of Life Science, Tokyo University of Pharmacy and Life Science, Hachioji, Japan.
Shibata
Mika
M
Kawashima
Hoshiko
H
Hatsuzawa
Kiyotaka
K
Nagahama
Masami
M
Tagaya
Mitsuo
M
Tani
Katsuko
K
D048868:Adaptor Proteins, Signal Transducing; D017398:Alternative Splicing; D002352:Carrier Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D002460:Cell Line; D011992:Endosomes / Q000648:ultrastructure; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D024101:Mitochondrial Proteins; D052067:Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins; D015534:Trans-Activators / Q000032:analysis / Q000235:genetics*; D033921:Vesicular Transport Proteins
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; C485422:RAB11FIP5 protein, human; D052067:Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins; D015534:Trans-Activators; D033921:Vesicular Transport Proteins