title: Association of single nucleotide polymorphisms in the growth hormone and growth hormone receptor genes with blood serum insulin-like growth factor I concentration and growth traits in Angus cattle.
abstract: This study was conducted to identify polymorphisms in the promoter and coding regions of the bovine growth hormone and growth hormone receptor genes and to study association of polymorphisms identified in these genes with growth traits and serum insulin-like growth factor-I (IGF-I) concentration. The denaturing gradient gel electrophoresis method and sequencing were utilized to identify three new single nucleotide polymorphisms in the promoter region of the growth hormone gene in Angus cattle. Polymerase chain reaction-based restriction fragment length polymorphism procedures were developed for rapid determination of the single nucleotide polymorphism genotypes in the growth hormone and the growth hormone receptor genes among Angus calves from lines divergently selected for high or low blood serum IGF-I concentration. The IGF-I concentration and growth traits were analyzed using animal models. The single nucleotide polymorphism in the promoter region of the growth hormone receptor gene was associated with serum IGF-I concentration on d 42 of the postweaning test and with mean IGF-I concentration. The associated effects of the markers need to be verified in other populations.
authors.affiliation: Department of Animal Sciences, The Ohio State University, Columbus 43210-1095, USA.
authors.forename: W
authors.initials: W
authors.lastname: Ge
authors.forename: M E
authors.initials: ME
authors.lastname: Davis
authors.forename: H C
authors.initials: HC
authors.lastname: Hines
authors.forename: K M
authors.initials: KM
authors.lastname: Irvin
authors.forename: R C M
authors.initials: RC
authors.lastname: Simmen
mesh_terms: D000818:Animals; D002417:Cattle / Q000097:blood / Q000235:genetics* / Q000254:growth & development*; D005819:Genetic Markers; D005838:Genotype; D013006:Growth Hormone / Q000235:genetics*; D007334:Insulin-Like Growth Factor I / Q000032:analysis*; D017354:Point Mutation / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D011986:Receptors, Somatotropin / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D011986:Receptors, Somatotropin; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone