title: Selective detection of fluorophore layers in turbid media: the role of fiber-optic probe design.
abstract: Experimental verification of the ability to alter the sensitivity to fluorophore layers in turbid media by varying illumination-collection geometry is presented. Fiber-optic probes and two-layer, fluorophore-doped, turbid phantoms are used to elucidate the roles of spot size, illumination-collection fiber separation, and probe-sample spacing. Variations in single- and multiple-fiber probe design parameters produce significant changes in the relative sensitivity to sample layers in a manner that agrees with prior computational studies.
authors.affiliation: Center for Devices and Radiological Health, Food and Drug Administration, 12725 Twinbrook Parkway, Rockville, Maryland 20857, USA. josh@eob.cdrh.fda.gov
authors.forename: T Joshua
authors.initials: TJ
authors.lastname: Pfefer
authors.forename: L Stephanie
authors.initials: LS
authors.lastname: Matchette
authors.forename: Amanda M
authors.initials: AM
authors.lastname: Ross
authors.forename: Marwood N
authors.initials: MN
authors.lastname: Ediger
mesh_terms: D004867:Equipment Design; D005336:Fiber Optic Technology / Q000295:instrumentation*; D019793:Fluorescein; D005456:Fluorescent Dyes / Q000493:pharmacokinetics*; D008962:Models, Theoretical; D055100:Optical Fibers; D019047:Phantoms, Imaging; D012235:Rhodamines
chemical_list: D005456:Fluorescent Dyes; D012235:Rhodamines; D019793:Fluorescein