title: Quantitative determination of ambroxol in tablets by derivative UV spectrophotometric method and HPLC.
abstract: A derivative UV spectrophotometric method for the determination of ambroxol in tablets was developed. Determination of ambroxol in tablets was conducted by using first-order derivative UV spectrophotometric method at 255 nm (n = 5). Standards for the calibration graph ranging from 5.0 to 35.0 microg/ml were prepared from stock solution. The proposed method was accurate with 98.6+/-0.4% recovery value and precise with coefficient of variation (CV) of 1.22. These results were compared with those obtained by reference methods, zero-order UV spectrophotometric method and reversed-phase high-performance liquid chromatography (HPLC) method. A reversed-phase C(18) column with aqueous phosphate (0.01 M)-acetonitrile-glacial acetic acid (59:40:1, v/v/v) (pH 3.12) mobile phase was used and UV detector was set to 252 nm. Calibration solutions used in HPLC were ranging from 5.0 to 20.0 microg/ml. Results obtained by derivative UV spectrophotometric method was comparable to those obtained by reference methods, zero-order UV spectrophotometric method and HPLC, as far as ANOVA test, F(calculated) = 0.762 and F(theoretical) = 3.89, was concerned.
authors.affiliation: Faculty of Pharmacy, Department of Analytical Chemistry, Gazi University, Etiler, Ankara 06330, Turkey.
authors.forename: Zafer
authors.initials: Z
authors.lastname: Dinçer
authors.forename: Hasan
authors.initials: H
authors.lastname: Basan
authors.forename: Nilgün Günden
authors.initials: NG
authors.lastname: Göger
mesh_terms: D000551:Ambroxol / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005100:Expectorants / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013607:Tablets / Q000737:chemistry*
chemical_list: D005100:Expectorants; D013607:Tablets; D000551:Ambroxol