Adeno-associated virus type 2 Rep40 modulates the proliferation rate of Rep52-expressing HeLa cells.
OBJECTIVE
To investigate the growth-inhibitory effect of Rep proteins on cells.


METHODS
We generated Rep-expressing plasmids that containing wild-type rep genes or mutant rep genes with point mutations in their ATP-binding sites. We obtained several HeLa cell clones expressing Rep proteins constitutively by the regulator plasmid. The expression of each Rep protein was detected by Western blotting with an anti-Rep monoclonal antibody.


RESULTS
Clones that expressed Rep52 and Rep40 grew more slowly than those which exhibited no detectable expression of Rep or mutant Rep protein. In contrast, clone w-33, which proliferated as quickly as control HeLa cells, expressed only Rep40. By comparing the expression levels of Rep proteins in these clones, we found that the ratio of Rep40 to Rep52 gradually increased in the faster growing clones.


CONCLUSIONS
These results suggest that while Rep52 expression retards cell proliferation, Rep40 promotes recovery from Rep52-induced HeLa cell growth inhibition.
Li
Donglan
D
Department of Transfusion Medicine, Kyoto University Hospital, Kyoto, Japan.
Kashii
Saburo
S
Sahara
Toshiyuki
T
Ito
Kazuhiko
K
D001483:Base Sequence; D002455:Cell Division; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D000229:Dependovirus / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D016195:G2 Phase; D015870:Gene Expression; D005814:Genes, Viral; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000502:physiology*; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology*; D014779:Virus Replication
D004268:DNA-Binding Proteins; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; C068734:rep proteins, Adeno-associated virus 2