title: Protein separations using colloidal magnetic nanoparticles.
abstract: Phospholipid-coated colloidal magnetic nanoparticles with mean magnetite core size of 8 nm are shown to be effective ion exchange media for the recovery and separation of proteins from protein mixtures. These particles have high adsorptive capacities (up to 1200 mg protein/mL adsorbent, an order of magnitude larger than the best commercially available adsorbents) and exhibit none of the diffusional resistances offered by conventional porous ion exchange media. Protein-laden particles are readily recovered from the feed solution using high-gradient magnetic filtration.
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
authors.forename: Seyda
authors.initials: S
authors.lastname: Bucak
authors.forename: Deverraux A
authors.initials: DA
authors.lastname: Jones
authors.forename: Paul E
authors.initials: PE
authors.lastname: Laibinis
authors.forename: T Alan
authors.initials: TA
authors.lastname: Hatton
mesh_terms: D000327:Adsorption; D002852:Chromatography, Ion Exchange / Q000379:methods*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry*; D003102:Colloids; D045304:Cytochromes c / Q000737:chemistry / Q000302:isolation & purification; D008081:Liposomes / Q000302:isolation & purification*; D008280:Magnetics; D008863:Microspheres; D036103:Nanotechnology / Q000379:methods*; D010743:Phospholipids / Q000737:chemistry; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D014462:Ultrafiltration / Q000379:methods*
chemical_list: D020099:Coated Materials, Biocompatible; D003102:Colloids; D008081:Liposomes; D010743:Phospholipids; D011506:Proteins; D045304:Cytochromes c