[Detection of SEN virus in sera of patients with chronic hepatitis B and general population in 5 cities of China].
OBJECTIVE
To study the prevalence of SEN virus (SENV) infection in CHB patients in five cities of China.


METHODS
A nest-polymerase chain reaction (nPCR) was used for detection of SENV-D and SENV-H in sera of 595 CHB patients from 5 cities of China and 96 normal individuals from Beijing. A total of 7 SENV strains were analyzed by direct sequencing.


RESULTS
The prevalence rates of SENV in CHB patients and normal individuals were 61.3% and 62.5%, respectively (chi(2) = 0.047, P = 0.829). The prevalence rates of CHB patients between 5 cities were different. Nucleotide sequence analysis showed that the homology between 4 SENV-D strains was 91% - 98% and 95% - 98% between 3 SENV-H strains isolated from 5 cities in China.


CONCLUSIONS
SENV-D/H were prevalent in CHB patients of China and their prevalence rates were similar to that in normal individuals.
Yan
Jie
J
Beijing Ditan Hospital, Beijing 100011, China.
He
Zhong-ping
ZP
Zhuang
Hui
H
Dong
Qing-ming
QM
Song
Shu-jing
SJ
Zhu
Lin
L
Wang
Xiao-hong
XH
D002681:China / Q000453:epidemiology; D018136:Circoviridae / Q000302:isolation & purification; D018173:Circoviridae Infections / Q000150:complications / Q000453:epidemiology / Q000821:virology; D004266:DNA Virus Infections / Q000150:complications / Q000453:epidemiology* / Q000821:virology; D004267:DNA Viruses / Q000302:isolation & purification*; D004279:DNA, Viral / Q000032:analysis; D019694:Hepatitis B, Chronic / Q000150:complications / Q000821:virology*; D006801:Humans; D010802:Phylogeny; D015995:Prevalence
D004279:DNA, Viral