[Biocompatibility of SA/CS-CaCl2/PMCG Microcapsule in cell culture].
A novel multi-components microcapsule--SA/CS-CaCl2/PMCG system was introduced. The effects of PMCG and SA/CS-CaCl2/PMCG microcapsules on the growth of free E. coli and Saccharomyces cerevisiae were studied respectively. In addition, the growth of immobilized E. coli and Saccharomyces cerevisiae were also investigated. The results showed that: Just like other synthetic polycations, PMCG above certain concentration (0.5%) strongly inhibited the growth of free E. coli and Saccharomyces cerevisiae, but SA/CS-CaCl2/PMCG microcapsules almost had no effects on their growth and on the consumption of glucose concentration by Saccharomyces cerevisiae. What's more, immobilized E. coli and Saccharomyces cerevisiae grew almost as normally as free cultivation. As a whole, SA/CS-CaCl2/PMCG microcapsules had good biocompatiability and can be used as a new immobilization system.
Zhang
Li-Yang
LY
Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, China.
Guan
Yi-Xin
YX
Yao
Shan-Jing
SJ
D000464:Alginates / Q000633:toxicity*; D001672:Biocompatible Materials / Q000633:toxicity*; D002122:Calcium Chloride / Q000633:toxicity*; D002482:Cellulose / Q000031:analogs & derivatives* / Q000633:toxicity*; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D011108:Polymers / Q000633:toxicity*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000254:growth & development
D000464:Alginates; D001672:Biocompatible Materials; D006603:Hexuronic Acids; D011108:Polymers; D020723:Glucuronic Acid; D002482:Cellulose; C020254:cellulose sulfate; D002122:Calcium Chloride