Purification of C-phycocyanin from Spirulina (Arthrospira) fusiformis.
C-phycocyanin was purified from Spirulina (Arthrospira) fusiformis by a multi-step treatment of the crude extract with rivanol in a ratio 10:1 (v/v), followed by 40% saturation with ammonium sulfate. After removal of rivanol by gel-filtration on Sephadex G-25, the pigment solution was saturated to 70% with ammonium sulfate. After the last step of purification, C-phycocyanin had an emission and absorption maxima at 620 and 650 nm, respectively and absorbance ratio A(620)/A(280) of 4.3, which are specific for the pure biliprotein. Its homogeneity was demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, yielding two bands of molecular masses 19500 and 21500 kDa, corresponding to alpha and beta subunits of the pigment, respectively. The yield of C-phycocyanin was approximately 46% from its content in the crude extract.
Minkova
K M
KM
Institute of Plant Physiology, 'Acad M Popov', Bulgarian Academy of Sciences, Acad G Bonchev Str, Bl 21, Sofia 1113, Bulgaria. kaledona@bio.bas.bg
Tchernov
A A
AA
Tchorbadjieva
M I
MI
Fournadjieva
S T
ST
Antova
R E
RE
Busheva
M Ch
MCh
D001426:Bacterial Proteins / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D004975:Ethacridine / Q000737:chemistry*; D008970:Molecular Weight; D010798:Phycocyanin / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification*; D053545:Spirulina; D014462:Ultrafiltration / Q000379:methods*
D001426:Bacterial Proteins; D010798:Phycocyanin; D004975:Ethacridine