title: Soymetide, an immunostimulating peptide derived from soybean beta-conglycinin, is an fMLP agonist.
abstract: A tridecapeptide (MITLAIPVNKPGR) that stimulates phagocytosis of human neutrophils was isolated from a trypsin digest of soybean proteins. This peptide is derived from the soybean beta-conglycinin alpha' subunit and was named soymetide-13. The N-terminal methionine residue of soymetide-13 is essential for its activity, and removal of C-terminal residues revealed that soymetide-4 (MITL) is the minimal structure required for phagocytosis stimulation. Although they are not formylated at their N-termini, soymetides have a weak affinity for the N-formyl-methionyl-leucyl-phenylalanine (fMLP) receptor and their phagocytosis-stimulating activity is inhibited by the fMLP antagonist Boc-MLP. Interestingly, soymetide-4 promotes tumor necrosis factor alpha production at a higher level than soymetide-13 following oral administration in mice.
authors.affiliation: Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Tsuruki
authors.forename: Katsuki
authors.initials: K
authors.lastname: Kishi
authors.forename: Masakazu
authors.initials: M
authors.lastname: Takahashi
authors.forename: Makoto
authors.initials: M
authors.lastname: Tanaka
authors.forename: Taiji
authors.initials: T
authors.lastname: Matsukawa
authors.forename: Masaaki
authors.initials: M
authors.lastname: Yoshikawa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D052179:Antigens, Plant; D005916:Globulins / Q000737:chemistry*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000819:agonists*; D055314:Seed Storage Proteins; D030262:Soybean Proteins / Q000737:chemistry*; D013025:Glycine max / Q000737:chemistry*; D013329:Structure-Activity Relationship; D013481:Superoxides / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D052179:Antigens, Plant; D005916:Globulins; D055314:Seed Storage Proteins; D030262:Soybean Proteins; D014409:Tumor Necrosis Factor-alpha; C013894:beta-conglycinin protein, Glycine max; D013481:Superoxides; D009240:N-Formylmethionine Leucyl-Phenylalanine