Glutamine preserves cardiomyocyte viability and enhances recovery of contractile function after ischemia-reperfusion injury.
BACKGROUND
Glutamine has been shown to protect against cellular injury in in vitro gut epithelial cells and in vivo in the septic rat. Glutamine's effect on the cardiomyocyte has not been explored. We tested the hypothesis that glutamine can enhance heat shock protein 72 (HSP 72) expression, attenuate intracellular oxidant generation, and protect cardiomyocytes against ischemia/reperfusion (I/R) injury.


METHODS
Chicken cardiomyocytes were supplemented with glutamine (10 mmol/L) or were controls (0 mmol/L). Cells underwent I/R, and HSP 72 content was evaluated using Western blotting. Reactive oxygen species generation was quantified through 2'-7'-dichlorofluorescin diacetate oxidation. Cell viability was quantified using propidium iodide staining. Return of contractile function was analyzed through phase contrast microscopy.


RESULTS
Glutamine significantly increased cardiomyocyte HSP 72 expression and markedly reduced cell death after I/R injury. Glutamine did not significantly decrease intracellular oxidant generation. Contractile function returned in all glutamine-treated cells versus none of the control cells postreperfusion.


CONCLUSIONS
Glutamine significantly increases cardiomyocytes survival and recovery of contractile function after I/R injury. This protection is associated with enhanced HSP 72 expression. These observations suggest that glutamine may prove beneficial as a protective therapy in patients at risk for cardiac ischemia and reperfusion injury, such as patients undergoing procedures requiring cardiopulmonary bypass and patients with coronary artery disease.
Wischmeyer
Paul E
PE
Department of Anesthesiology, University of Colorado, Denver 80262, USA. Paul.Wischmeyer@UCHSC.edu
Vanden Hoek
Terry L
TL
Li
Changquiung
C
Shao
Zuohui
Z
Ren
Hongyu
H
Riehm
Jacob
J
Becker
Lance B
LB
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002645:Chickens; D005452:Fluoresceins; D005973:Glutamine / Q000008:administration & dosage / Q000494:pharmacology*; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000378:metabolism*; D008858:Microscopy, Phase-Contrast; D009200:Myocardial Contraction / Q000187:drug effects*; D017202:Myocardial Ischemia / Q000188:drug therapy* / Q000378:metabolism; D015425:Myocardial Reperfusion; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D015427:Reperfusion Injury
D005452:Fluoresceins; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D017382:Reactive Oxygen Species; D005973:Glutamine; C037631:2',7'-dichlorofluorescein