Study of rat osteoblasts transfected by transforming growth factor beta 1 gene.
In order to investigate the effect of TGF beta 1 gene transfer on the biological characteristics, the effects of gene transfer and supernatant of transfected osteoblasts on the proliferation and ALP activity of osteoblasts were detected by 3H-TdR and MTT. Our results showed that TGF beta 1 gene transfer had no effect on the biological characteristics and the activated supernatant of transfected osteoblasts stimulated proliferation and inhibited ALP activity of osteoblasts. TGF beta 1 gene transfer could promote the expression of TGF beta 1 and the biological characteristics of transfected osteoblasts were stable, which might be helpful for gene therapy of bone defects in vivo.
Liu
Yong
Y
Department of Orthopedics, Xiehe Hospital, Tongji Medical University, Huazhong University of Science and Technology, Wuhan 430022.
Du
Jingyuan
J
Zheng
Qixin
Q
Wang
Hong
H
Guo
Xiaodong
X
Duan
Deyu
D
Liu
Weigang
W
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000235:genetics; D018014:Gene Transfer Techniques; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D023822:Tissue Engineering; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000235:genetics*
D018076:DNA, Complementary; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta