Flow injection chemiluminescent assays for glycerol and triglycerides using a co-immobilized enzyme reactor.
A flow injection method for the determination of glycerol using a co-immobilized enzyme reactor containing glycerokinase and glycerol-3-phosphate oxidase is described. The hydrogen peroxide produced is monitored by using a luminol chemiluminescence reaction in the presence of catalyst such as Co(II). The detection limit (2.5 x blank noise) for glycerol is 7 x 10(-3) mmol/L with a sample throughput of 40/h. The calibration graph is linear over the range studied (0.2-1.0 mmol/L) with relative standard deviation 1.2-2.4%. The method is applied to the determination of glycerol in blood serum produced off-line from triglycerides using lipase isolated from bovine pancreas.
Yaqoob
M
M
Department of Chemistry, University of Balochistan, Quetta, Pakistan. yaqoob2001@hotmail.com
Nabi
A
A
D000818:Animals; D002138:Calibration; D002417:Cattle; D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized / Q000737:chemistry*; D017022:Flow Injection Analysis; D005990:Glycerol / Q000032:analysis* / Q000097:blood; D005991:Glycerol Kinase / Q000737:chemistry*; D005993:Glycerolphosphate Dehydrogenase / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry; D020799:Pancrelipase / Q000737:chemistry; D014280:Triglycerides / Q000032:analysis* / Q000097:blood
D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D007202:Indicators and Reagents; D014280:Triglycerides; D020799:Pancrelipase; D008165:Luminol; D006861:Hydrogen Peroxide; D005993:Glycerolphosphate Dehydrogenase; C036140:L-alpha-glycerol-phosphate oxidase; D005991:Glycerol Kinase; D005990:Glycerol