Purification and characterization of a lectin from Annona coriacea seeds.
A novel lectin, denominated ACLEC, was isolated from Annona coriacea seeds, belonging to the Annonaceae family. The lectin presented one protein band in SDS-PAGE of 14 kDa. Of the sugars tested, Dglucose and D-mannose were the best inhibitors. A search sequence database showed that ACLEC had homology with other plant lectins, belonging to leguminous lectin family.
Coelho
Mirela B
MB
Departmento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas, SP, Brazil.
Freire
Maria das Graças M
Md
Toyama
Marcos H
MH
Marangoni
Sérgio
S
Novello
José C
JC
Macedo
Maria Lígia R
ML
D000818:Animals; D031041:Annona / Q000737:chemistry* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000378:metabolism; D006801:Humans; D037102:Lectins / Q000737:chemistry* / Q000378:metabolism; D012639:Seeds / Q000737:chemistry* / Q000378:metabolism
D037102:Lectins