[Screening and application of human-derived HBsAg bacteriophage single chain antibody in clinical diagnosis].
OBJECTIVE
To identify human single chain Fv antibody (ScFv) against hepatitis B viral surface antigen.


METHODS
The recombinant phages were panned by HBsAg which was coated in a microtiter plate, after five rounds of biopanning, 56 phage clones were identified specific to HBsAg. The specificity of ScFv was evaluated by ELISA and immunohistochemistry, respectively.


RESULTS
The data of HB sAg-ScFv DNA digestion and DNA sequencing showed that the ScFv gene is composed of 750 bp. ELISA and immunohistochemistry demonstrated that the human single chain Fv antibody against hepatitis B surface antigen has a specific combination character with hepatitis B surface antigen of different sources and paraffin-embedded patients tissue specimens, it did not react with normal liver tissue and HCV.


CONCLUSIONS
The application of HBsAg specific ScFv in immunohistochemistry was successfully achieved.
Zhong
Yanwei
Y
Gene Therapy Research Centre, Institute of Infectious Diseases, The 302nd Hospital of PLA, Beijing 100039, China.
Cheng
Jun
J
Shi
Shuangshuang
S
Zhao
Jingmin
J
Wang
Gang
G
Xia
Xiaobing
X
Tian
Xiaojun
X
Li
Li
L
Zhang
Lingxia
L
D001435:Bacteriophages / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D019694:Hepatitis B, Chronic / Q000175:diagnosis*; D006801:Humans; D019151:Peptide Library
D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D019151:Peptide Library