Identification of a truncated Tn1721-like transposon located on a small plasmid of Escherichia coli isolated from Varanus indicus.
The 9.1 kb plasmid pDEWT1 was isolated from an Escherichia coli strain obtained from the faeces of a free-living lizard (Varanus indicus) in Indonesia. This plasmid mediated tetracycline resistance via a tet gene of hybridization class A. Molecular analysis of a 7755 bp segment of plasmid pDEWT1 including the tetR-tet(A) region and its flanking areas suggested that pDEWT1 harboured a truncated copy of the tet(A)-carrying transposon Tn1721 in which the part responsible for chemotaxis and transposition functions was lost. Analysis of the sequences at the integration site revealed the presence of the 5-bp direct repeat TACTT. The sequences upstream and downstream of the integration site showed striking homology to sequences of a non-coding region detectable on a small cryptic plasmid from Yersinia enterocolitica.
Waturangi
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DE
Department of Biology, Faculty of Science and Mathematics, IUC Biotechnology, Bogor Agricultural University, Indonesia.
Schwarz
S
S
Suwanto
A
A
Kehrenberg
C
C
Erdelen
W
W
D000818:Animals; D001483:Base Sequence; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D008116:Lizards / Q000382:microbiology*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D013753:Tetracycline Resistance / Q000235:genetics*
D004251:DNA Transposable Elements; D004269:DNA, Bacterial