Activation of neurons in trigeminal caudalis by noxious oral acidic or salt stimuli is not reduced by amiloride.
We investigated the possible role of amiloride-sensitive ion channels of the ENaC/DEGenerin superfamily in the activation of trigeminal nociceptive neurons elicited by noxious chemical stimulation of the oral mucosa using two methodologies, single-unit recording and c-fos immunohistochemistry. In pentobarbital-anesthetized rats, single-unit recordings were made from neurons in superficial laminae of dorsomedial trigeminal subnucleus caudalis (Vc) that responded to noxious thermal and chemical stimuli applied to the dorsal tongue. Successive application of each of three chemicals (250 mM pentanoic acid, n=6 units; 250 mM citric acid, n=8; 5 M NaCl, n=6) evoked responses that were not affected following topical application of amiloride (1 mM). In separate experiments, pentobarbital-anesthetized rats received one of the following stimuli delivered to the dorsal tongue: 250 mM pentanoic acid (n=6); 1 mM amiloride followed by 250 mM pentanoic (N=6); 5 M NaCl (n=5); or 1 mM amiloride followed by 5 M NaCl (n=5). Two hours later they were perfused with 4% paraformaldehyde and the brain stems processed for c-fos immunoreactivity. Both pentanoic acid and 5 M NaCl evoked similar numbers and patterns of fos-like immunoreactivity (FLI) in dorsomedial Vc and other brain stem regions, with no significant difference in counts of FLI in animals pretreated with amiloride. These results suggest that amiloride-sensitive Na(+) channels are not essential in mediating the activation of intraoral trigeminal nociceptors.
Sudo
Satoko
S
Department of Anesthesiology and Resuscitology, Ehime University School of Medicine, Ehime, Shigenobu, Japan.
Sudo
Makoto
M
Simons
Christopher T
CT
Dessirier
Jean Marc
JM
Iodi Carstens
Mirela
M
Carstens
E
E
D000584:Amiloride / Q000494:pharmacology*; D000818:Animals; D001931:Brain Mapping; D002614:Chelating Agents / Q000494:pharmacology; D004232:Diuretics / Q000494:pharmacology*; D004594:Electrophysiology; D053503:Epithelial Sodium Channels; D007150:Immunohistochemistry; D007509:Irritants / Q000494:pharmacology*; D008297:Male; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D009619:Nociceptors / Q000378:metabolism; D018408:Patch-Clamp Techniques; D010421:Pentanoic Acids / Q000494:pharmacology; D016760:Proto-Oncogene Proteins c-fos / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015222:Sodium Channels / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D014059:Tongue / Q000294:innervation; D014275:Trigeminal Caudal Nucleus / Q000187:drug effects / Q000378:metabolism
D002614:Chelating Agents; D004232:Diuretics; D053503:Epithelial Sodium Channels; D007509:Irritants; D010421:Pentanoic Acids; D016760:Proto-Oncogene Proteins c-fos; D015222:Sodium Channels; D012965:Sodium Chloride; D000584:Amiloride