title: Apolipoprotein E and apolipoprotein CI polymorphisms in the Czech population: almost complete linkage disequilibrium of the less frequent alleles of both polymorphisms.
abstract: Apolipoproteins E and CI are the predominant components of triglyceride-rich lipoproteins. The genes are located in one gene cluster and both are polymorphic. Three allelic (epsilon2, epsilon3 and epsilon4) polymorphisms of the APOE gene influence plasma cholesterol levels. The distribution of these alleles differ between ethnic groups. PCR genotyping was used to determine the APOE and APOCI allele incidence in a representative group of 653 probands (302 men and 351 women) of Czech origin. The observed relative frequencies for the epsilon2, epsilon3 and epsilon4 alleles were 7.1 %, 82.0 % and 10.9 %, respectively, and are similar to other middle European populations. APO epsilon4 carriers have the highest and APO epsilon2 carriers the lowest levels of plasma total cholesterol (p<0.0001) and LDL cholesterol (p<0.0001). The frequency of the insertion (I) allele (HpaI restriction site present) of the APOCI polymorphism was 18.5 %. APOCI I/I homozygotes have the highest level of triglycerides (p<0.003). An almost complete linkage disequilibrium of the insertion allele of APOCI with the APOE alleles epsilon2 and epsilon4 has been detected and suggests that the deletion in the APOCI gene probably follows the deriving of all three APOE alleles on the APO epsilon3 allele background.
authors.affiliation: Laboratory of Atherosclerosis Research, Institute for Clinical and Experimental Medicine, 140 21 Prague 4, Czech Republic. jaroslav.hubacek@medicon.cz
authors.forename: J A
authors.initials: JA
authors.lastname: Hubácek
authors.forename: J
authors.initials: J
authors.lastname: Pitha
authors.forename: V
authors.initials: V
authors.lastname: Adámková
authors.forename: Z
authors.initials: Z
authors.lastname: Skodová
authors.forename: V
authors.initials: V
authors.lastname: Lánská
authors.forename: R
authors.initials: R
authors.lastname: Poledne
mesh_terms: D053302:Apolipoprotein C-I; D001056:Apolipoproteins C / Q000235:genetics*; D001057:Apolipoproteins E / Q000235:genetics*; D015331:Cohort Studies; D018153:Czech Republic / Q000453:epidemiology; D005260:Female; D005787:Gene Frequency / Q000235:genetics*; D020022:Genetic Predisposition to Disease / Q000453:epidemiology* / Q000208:ethnology; D006801:Humans; D015810:Linkage Disequilibrium / Q000235:genetics*; D008055:Lipids / Q000097:blood*; D008074:Lipoproteins / Q000097:blood; D008297:Male; D011110:Polymorphism, Genetic; D012737:Sex Factors
chemical_list: D053302:Apolipoprotein C-I; D001056:Apolipoproteins C; D001057:Apolipoproteins E; D008055:Lipids; D008074:Lipoproteins