title: Experimental study of the effect of autonomic nervous system on the transmural dispersion of ventricular repolarization under acute myocardial ischemia in vivo.
abstract: The effect of the autonomic nerves on the transmural dispersion of ventricular repolarization (TDR) under acute myocardial ischemia in intact canine was investigated. Using the monophasic action potential (MAP) recording technique, MAPs of the epicardium (Epi), mid-myocardium (Mid) and endocardium (Endo) were recorded simultaneously by specially designed plunge-needle electrodes at the left ventricular free wall under acute myocardial ischemia in 12 open-chest dogs. MAPD90 and TDR among three myocardial layers as well as the incidence of the early afterdepolarization (EAD) before autonomic nervous stimulation and during autonomic nervous stimulation were compared. It was found that 10 min after acute myocardial ischemia, TDR was increased from 55 +/- 8 ms to 86 +/- 15 ms during sympathetic stimulation (P < 0.01). The TDR (53 +/- 9 ms) during parasympathetic stimulation was not significantly different from that of the control (55 +/- 8 ms) (P > 0.05). The EAD was elicited in the Mid of 2 dogs (16%) 10 min after acute myocardial ischemia, but the EAD were elicited in the Mid of 7 dogs (58%) during sympathetic stimulation (P < 0.01). It was concluded that: (1) Sympathetic stimulation can increase the transmural dispersion of repolarization and induce early afterdepolarizations in the Mid under acute myocardial ischemia, which provide the opportunity for the ventricular arrhythmia developing; (2) Parasympathetic stimulation has no significant effect on the transmural dispersion of repolarization under myocardial ischemia.
authors.affiliation: Department of Cardiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
authors.forename: Cuntai
authors.initials: C
authors.lastname: Zhang
authors.forename: Dawen
authors.initials: D
authors.lastname: Xu
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Nian
authors.initials: N
authors.lastname: Liu
authors.forename: Jianghua
authors.initials: J
authors.lastname: Zhong
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Zaiying
authors.initials: Z
authors.lastname: Lu
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000818:Animals; D001341:Autonomic Nervous System / Q000503:physiopathology*; D004285:Dogs; D004558:Electric Stimulation; D005260:Female; D006352:Heart Ventricles / Q000294:innervation; D008297:Male; D017202:Myocardial Ischemia / Q000503:physiopathology*; D009469:Neuromuscular Junction; D016276:Ventricular Function