title: Renal blood flow in hypercholesterolemic pigs is increased by chronic antioxidant treatment.
abstract: Oxygen radical species can influence vascular tone, and antioxidants may have hemodynamic and vascular effects. To date, the vascular effects of chronic intervention with a combination of antioxidant vitamins E and C on renal blood flow (RBF) in hypercholesterolemia (which increases oxidative stress) have not been fully defined. The aim of this intervention study was to explore the involvement of increased oxidative stress in pig RBF disturbance by using chronic dietary antioxidant vitamin intervention. Responses of RBF to the acetylcholine (Ach) were measured in vivo using electron beam computed tomography (EBCT). Acetylcholine significantly increased RBF in normal and hypercholesterolemic + vitamins (P < 0.05 for both), but not in hypercholesterolemic pigs (P=0.1). In normocholesterolemic + vitamins pigs, Ach infusion did not induce any further increase in RBF, but RBF was similar to that observed in normal and hypercholesterolemic + vitamins under the same conditions, and tended to be higher than in hypercholesterolemic pigs (P=0.06). Thus, antioxidants improve RBF in hypercholesterolemic pigs and this effect may help to prevent renal diseases and hypertension in animals.
authors.affiliation: Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Sassari, Italy.
authors.forename: F
authors.initials: F
authors.lastname: De Nigris
authors.forename: M P
authors.initials: MP
authors.lastname: Demontis
authors.forename: M
authors.initials: M
authors.lastname: Rodriguez-Porcel
authors.forename: V
authors.initials: V
authors.lastname: Anania
authors.forename: L O
authors.initials: LO
authors.lastname: Lerman
authors.forename: C
authors.initials: C
authors.lastname: Napoli
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D002784:Cholesterol / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D019587:Dietary Supplements; D006937:Hypercholesterolemia / Q000000981:diagnostic imaging / Q000188:drug therapy* / Q000503:physiopathology; D011859:Radiography; D012079:Renal Circulation / Q000187:drug effects*; D013552:Swine; D013553:Swine Diseases / Q000000981:diagnostic imaging / Q000188:drug therapy* / Q000503:physiopathology; D014810:Vitamin E / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D000975:Antioxidants; D008078:Cholesterol, LDL; D014810:Vitamin E; D002784:Cholesterol; D000109:Acetylcholine; D001205:Ascorbic Acid