Broth recycling to reduce process noise resulting from concentrated substrate addition in fed-batch cultivation of Escherichia coli.
In this work feed hardware for fed-batch cultivation is presented (broth recycle feed injection system or BRFIS). BRFIS proved superior to conventional submerged or dripped feed systems in reducing dissolved oxygen (DO) oscillations during Escherichia coli fed-batch cultivation (5 min coefficient of variation of 0.7% for BRFIS as compared to 26% or greater for conventional feeding hardware in a 2 L test reactor). Hence, BRFIS is useful for fed-batch cultivation systems where the DO signal is used in measurement or control.
Johnston
Wayne A
WA
School of Engineering, Murdoch University, Perth 6168, Western Australia.
Cooney
Michael J
MJ
D019149:Bioreactors; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000706:statistics & numerical data; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D005246:Feedback; D005947:Glucose / Q000378:metabolism; D009991:Oscillometry / Q000295:instrumentation / Q000379:methods*; D010100:Oxygen / Q000378:metabolism; D010101:Oxygen Consumption / Q000502:physiology*; D012212:Rheology / Q000295:instrumentation* / Q000379:methods; D013269:Stochastic Processes
D005947:Glucose; D010100:Oxygen