Structure and mechanism of soluble glucose dehydrogenase and other PQQ-dependent enzymes.
This paper discusses recent X-ray structures of several pyrroloquinoline quinone (PQQ)-dependent proteins in relation to their proposed modes of action. In addition, a detailed analysis of redox-related structural changes in the soluble PQQ-dependent glucose dehydrogenase is presented. A sequence comparison of that enzyme with a number of homologues shows that PQQ-dependent enzymes are much more widespread than has been assumed so far. In particular, the presence of a PQQ-dependent enzyme in at least one archaeon opens up the possibility that PQQ has been involved in prokaryotic metabolism since the early days of the evolution of bacterial life on earth.
Oubrie
Arthur
A
School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK. a.oubrie@uea.ac.uk
D000595:Amino Acid Sequence; D002384:Catalysis; D018360:Crystallography, X-Ray; D005948:Glucose Dehydrogenases / Q000737:chemistry*; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D045542:PQQ Cofactor; D011487:Protein Conformation; D015363:Quinolones / Q000378:metabolism*; D011809:Quinones / Q000378:metabolism*
D015363:Quinolones; D011809:Quinones; D045542:PQQ Cofactor; D005948:Glucose Dehydrogenases