IL-11 up-regulates Tie-2 expression during the healing of gastric ulcers in rats.
OBJECTIVE
To investigate Tie-2 expression during the repair of acetic acid-induced gastric ulcers in rats treated with recombinant human IL-11 (rhIL-11) and in untreated control animals.


METHODS
Gastric ulcers were induced in male Wistar rats by applying acetic acid to the fundus of the stomach. RhIL-11 (100 microg/kg twice daily, subcutaneously) was administered from two days before ulcer induction and continued for five days after the induction. Control rats received bovine serum albumin. Gastric specimens were collected at 3 and 5 days after the induction of ulcer for immunohistochemical observation, Western blotting, and reverse transcription polymerase chain reaction (RT-PCR).


RESULTS
Immunohistochemical and Western blot analysis demonstrated that Tie-2 expression was enhanced in the rhIL-11-treated rats compared with the control animals at both intervals.


CONCLUSIONS
These findings suggested that IL-11 could accelerate ulcer healing, in part, by up-regulating Tie-2 expression and promoting angiogenesis.
Wen
Chun-Yang
CY
Department of Molecular Pathology, Atomic Bomb Disease Institute, Nagasaki University School of Medicine, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan. cywen518@net.nagasaki-u.ac.jp
Ito
Masahiro
M
Wang
Hui
H
Chen
Long-Dian
LD
Xu
Zhao-Min
ZM
Matsuu
Mutsumi
M
Shichijo
Kazuko
K
Nakayama
Toshiyuki
T
Nakashima
Masahiro
M
Sekine
Ichiro
I
D000818:Animals; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000494:pharmacology*; D008297:Male; D051381:Rats; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics*; D042787:Receptor, TIE-2; D011994:Recombinant Proteins / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013276:Stomach Ulcer / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D014945:Wound Healing / Q000187:drug effects*
D007375:Interleukin-1; D011994:Recombinant Proteins; D020794:Receptor Protein-Tyrosine Kinases; D042787:Receptor, TIE-2