title: Crystallization and preliminary X-ray diffraction study of a wheat (Triticum aestivum L.) TAXI-type endoxylanase inhibitor.
abstract: A TAXI-type endoxylanase inhibitor from T. aestivum L. wheat flour has been crystallized using the hanging-drop vapour-diffusion method. The needle-like crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 49.92, b = 66.45, c = 106.42 A. From these crystals, a native data set and a gold-derivative data set were collected to 2.25 and 1.75 A resolution, respectively. The heavy-atom derivative of this crystal form was obtained by the soaking method and allowed determination of the initial phases.
authors.affiliation: Laboratorium voor Analytische Chemie en Medicinale Fysiochemie, K.U. Leuven, Belgium.
authors.forename: Stefaan
authors.initials: S
authors.lastname: Sansen
authors.forename: Christel
authors.initials: C
authors.lastname: Verboven
authors.forename: Camiel J
authors.initials: CJ
authors.lastname: De Ranter
authors.forename: Kurt
authors.initials: K
authors.lastname: Gebruers
authors.forename: Kristof
authors.initials: K
authors.lastname: Brijs
authors.forename: Christophe M
authors.initials: CM
authors.lastname: Courtin
authors.forename: Jan A
authors.initials: JA
authors.lastname: Delcour
authors.forename: Anja
authors.initials: A
authors.lastname: Rabijns
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D005433:Flour / Q000032:analysis; D007527:Isoenzymes / Q000737:chemistry; D010940:Plant Proteins / Q000138:chemical synthesis / Q000494:pharmacology; D013379:Substrate Specificity; D014908:Triticum / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D004791:Enzyme Inhibitors; D007527:Isoenzymes; D010940:Plant Proteins