Projections from the nucleus reticularis magnocellularis to the rat cervical cord using electrical stimulation and iontophoretic injection methods.
The aim of this study is to clarify the fiber distribution of the nucleus reticularis magnocellularis (NRMC) and adjacent areas in the rat spinal cord. Biotinylated dextran amine was injected iontophoretically through a glass capillary into the areas, in which a single cell responded to noxious electrical stimulation of the sciatic nerve and to a pinch of the thigh skin with multiple spikes. Labeled fibers descended bilaterally through the ventral funiculi of the medulla oblongata and then through the ventral and lateral funiculi of the cervical cord with an ipsilateral predominance, and terminated in the spinal gray (laminae I-X). A single fiber sometimes ran through several laminae while bifurcating many short branches with axon varicosities and terminal buttons in one transverse section, that is, through laminae V, VII and X, through laminae V, IIl-IV and I-II, and through laminae VII to I-II. The present study showed that the wide distribution of a single fiber and a mass of fibers descending from the NRMC and adjacent areas might modulate not only somatic sensory and motor functions but also autonomic functions in the spinal cord.
Watanabe
Shigeo
S
Department of Anatomy, Tokyo Medical University, Tokyo, Japan.
Kitamura
Taiko
T
Watanabe
Lisa
L
Sato
Hitoshi
H
Yamada
Jinzo
J
D000200:Action Potentials / Q000502:physiology; D000344:Afferent Pathways / Q000502:physiology; D000818:Animals; D001369:Axons / Q000502:physiology / Q000648:ultrastructure*; D001710:Biotin / Q000031:analogs & derivatives*; D003911:Dextrans; D004525:Efferent Pathways / Q000166:cytology* / Q000502:physiology; D004558:Electric Stimulation; D007839:Functional Laterality / Q000502:physiology; D008526:Medulla Oblongata / Q000166:cytology* / Q000502:physiology; D009431:Neural Conduction / Q000502:physiology; D009619:Nociceptors / Q000502:physiology; D010146:Pain / Q000503:physiopathology*; D017729:Presynaptic Terminals / Q000502:physiology / Q000648:ultrastructure; D051381:Rats; D011930:Reaction Time; D012154:Reticular Formation / Q000166:cytology* / Q000502:physiology; D013116:Spinal Cord / Q000166:cytology* / Q000502:physiology
D003911:Dextrans; C076397:biotinylated dextran amine; D001710:Biotin