Identification of 14 new phosphoproteins involved in important plant mitochondrial processes.
Protein phosphorylation is a very important posttranslational modification the role of which is practically unexplored in mitochondria. Using two-dimensional gel electrophoresis followed by mass spectrometry, 14 new phosphoproteins are identified in potato tuber mitochondria, all household proteins also present in mammalian and fungal mitochondria. Seven of the new phosphoproteins are involved in the tricarboxylic acid cycle or associated reactions, four are subunits of respiratory complexes and involved in electron transport, ATP synthesis and protein processing, two are heat shock proteins and one is involved in defence against oxidative stress. These findings open up entirely new possibilities for the regulation and signal integration of mitochondrial processes.
Bykova
Natalia V
NV
Plant Research Department, Risø National Laboratory, Bldg 301, PO Box 49, DK-4000 Roskilde, Denmark.
Egsgaard
Helge
H
Møller
Ian M
IM
D000255:Adenosine Triphosphate / Q000096:biosynthesis; D002952:Citric Acid Cycle; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D010750:Phosphoproteins / Q000737:chemistry / Q000502:physiology*; D010940:Plant Proteins / Q000737:chemistry / Q000502:physiology*
D010750:Phosphoproteins; D010940:Plant Proteins; D000255:Adenosine Triphosphate