Effect of sodium fluoride on the murine splenic immune response to Porphyromonas gingivalis in vitro.
Spleen cells from saline- and Porphyromonas gingivalis-primed mice were cultured and stimulated with or without P. gingivalis and added with or without various concentration of sodium fluoride (NaF). Cell proliferation, antigen-specific IgG antibodies and both IFN-gamma and IL-10 levels were determined by a colorimetric assay, ELISA and commercial ELISA kits respectively. The results showed that NaF at concentration of 1 x 10(-6) M enhanced but at concentration of 1 x 10(-1) M abolished the immune response to P. gingivalis, suggesting that NaF at low concentration may act as an adjuvant but at high concentration may be toxic to the P. gingivalis-induced murine splenic immune response in vitro.
Sosroseno
Wihaskoro
W
Department of Oral Biology, School of Dental Sciences, a Universiti Sains Malaysia, Kota Bharu, Malaysia. wihaskoro@kb.usm.my
D000818:Animals; D002478:Cells, Cultured; D016207:Cytokines / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007109:Immunity / Q000187:drug effects; D016753:Interleukin-10 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D017861:Porphyromonas / Q000276:immunology*; D012969:Sodium Fluoride / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000276:immunology
D016207:Cytokines; D016753:Interleukin-10; D012969:Sodium Fluoride