Homocysteine alters monocyte-endothelial interaction in vitro.
OBJECTIVE
To determine whether homocysteine induced endothelial damage through monocyte-endothelial interaction and to characterize both cell types in vitro.


METHODS
Radiomethods were performed on monocyte adhesion to/through endothelium and endothelial damage experiments.


RESULTS
Homocysteine-treated endothelial cells increased monocyte adhesion and transmigration. Homocysteine-treated monocytes induced endothelial detachment, but this effect was blocked by catalase. These effects were increased with higher concentrations of homocysteine. Monocyte surface glycoprotein antibodies CD11b/CD18 and CD14 inhibited these processes.


CONCLUSIONS
Homocysteine alters monocyte-endothelial interaction in vitro, eventually bringing about endothelial damage through release of H(2)O(2). These phenomena are mediated through monocyte surface glycoproteins CD11b/CD18 and CD14. Upregulation of these processes in vivo may contribute to acceleration of atherosclerosis in patients with elevated plasma homocysteine levels.
Guo
Xuewei
X
Center of Cardiovascular Disease Research, Gansu Province People's Hospital, Lanzhou 730000, China. Xueweiguo@163.net
Dudman
Nicholas Peter
NP
D001161:Arteriosclerosis / Q000209:etiology; D002448:Cell Adhesion / Q000187:drug effects; D002450:Cell Communication / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000166:cytology* / Q000187:drug effects*; D006710:Homocysteine / Q000494:pharmacology*; D006801:Humans; D009000:Monocytes / Q000187:drug effects* / Q000502:physiology
D006710:Homocysteine