title: Approaches to selectivity in the Zn(II)-phthalocyanine-photosensitized inactivation of wild-type and antibiotic-resistant Staphylococcus aureus.
abstract: A number of Zn(II)- phthalocyanines bearing peripheral substituents of cationic nature due to the presence of quaternarized anilinium or ammonium groups were shown to be efficient photoantimicrobial agents: a 4-5 log decrease in the survival of both wild-type or methicillin-resistant Staphylococcus aureus was obtained upon short irradiation times in the presence of phthalocyanine concentrations as low as 0.1 microM. A careful selection of the experimental protocol, and in particular the use of short (5 min) incubation times and mild irradiation parameters, allowed one to achieve a high selectivity of S. aureus photoinactivation as compared with important constituents of potential host tissues, such as human fibroblasts and keratinocytes. The efficiency and selectivity of the photoprocess were not affected by the presence of 5% human serum.
authors.affiliation: Molteni Farmaceutici S.S 67 Loc. Granatieri-50018 Scandicci, Firenze, Italy.
authors.forename: Marina
authors.initials: M
authors.lastname: Soncin
authors.forename: Clara
authors.initials: C
authors.lastname: Fabris
authors.forename: Alessandra
authors.initials: A
authors.lastname: Busetti
authors.forename: Donata
authors.initials: D
authors.lastname: Dei
authors.forename: Daniele
authors.initials: D
authors.lastname: Nistri
authors.forename: Gabrio
authors.initials: G
authors.lastname: Roncucci
authors.forename: Giulio
authors.initials: G
authors.lastname: Jori
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D024881:Drug Resistance, Bacterial; D005347:Fibroblasts / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D007211:Indoles / Q000737:chemistry / Q000494:pharmacology; D054833:Isoindoles; D015603:Keratinocytes / Q000187:drug effects / Q000528:radiation effects; D009942:Organometallic Compounds / Q000737:chemistry / Q000494:pharmacology; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000737:chemistry* / Q000494:pharmacology; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000528:radiation effects*; D013329:Structure-Activity Relationship; D017967:Zinc Compounds
chemical_list: D000900:Anti-Bacterial Agents; D007211:Indoles; D054833:Isoindoles; D009942:Organometallic Compounds; D017319:Photosensitizing Agents; D017967:Zinc Compounds; C052159:Zn(II)-phthalocyanine