Crystallization and preliminary X-ray diffraction study of a wheat (Triticum aestivum L.) TAXI-type endoxylanase inhibitor.
A TAXI-type endoxylanase inhibitor from T. aestivum L. wheat flour has been crystallized using the hanging-drop vapour-diffusion method. The needle-like crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 49.92, b = 66.45, c = 106.42 A. From these crystals, a native data set and a gold-derivative data set were collected to 2.25 and 1.75 A resolution, respectively. The heavy-atom derivative of this crystal form was obtained by the soaking method and allowed determination of the initial phases.
Sansen
Stefaan
S
Laboratorium voor Analytische Chemie en Medicinale Fysiochemie, K.U. Leuven, Belgium.
Verboven
Christel
C
De Ranter
Camiel J
CJ
Gebruers
Kurt
K
Brijs
Kristof
K
Courtin
Christophe M
CM
Delcour
Jan A
JA
Rabijns
Anja
A
D003460:Crystallization; D018360:Crystallography, X-Ray; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D005433:Flour / Q000032:analysis; D007527:Isoenzymes / Q000737:chemistry; D010940:Plant Proteins / Q000138:chemical synthesis / Q000494:pharmacology; D013379:Substrate Specificity; D014908:Triticum / Q000737:chemistry*; D014961:X-Ray Diffraction
D004791:Enzyme Inhibitors; D007527:Isoenzymes; D010940:Plant Proteins