Evidence that nitric oxide does not directly contribute to methacholine-induced amylase secretion in rabbit parotid acinar cells.
Nitric oxide (NO) is a short-lived free radical and is a widespread intra- and intercellular messenger molecule involved in various physiological functions. We have demonstrated previously that the muscarinic agonist methacholine induces endogenous generation of NO in rabbit parotid acinar cells. Since methacholine also simultaneously evokes amylase secretion, we investigated the effect of NO on the methacholine-induced exocytotic amylase secretion in rabbit parotid acinar cells. Methacholine-evoked amylase secretion was clearly reduced in the absence of extracellular Ca(2+). The Ca(2+)-mobilizing reagents A23187 and thapsigargin, which stimulate NO generation, also evoked amylase secretion. This response seemed to be caused by NO generated by the activation of endogenous Ca(2+)-regulated NO synthase. However, N(G)-nitro-L-arginine methyl ester (L-NAME), a specific NOS inhibitor, and the NO scavenger haemoglobin had no effect on methacholine-induced amylase secretion. The NO generator sodium nitroprusside (SNP) failed to evoke amylase release. We further studied the effects of L-NAME and SNP on methacholine-induced amylase secretion in crudely dispersed parotid gland cell clusters containing nerve tissue. In this preparation, L-NAME inhibited methacholine-induced amylase secretion and SNP evoked amylase secretion. It is thus unlikely that NO contributes directly to methacholine-induced amylase secretion in rabbit parotid acinar cells. NO appears rather to affect to nerve tissues in the cell suspension.
Tsunoda
Shoji
S
Department of Physiology, Nihon University School of Dentistry at Matsudo, Chiba 271-858 Matsudo, Japan.
Michikawa
Hiromi
H
Furuyama
Shunsuke
S
Sugiya
Hiroshi
H
D000681:Amylases / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006454:Hemoglobins / Q000494:pharmacology; D066298:In Vitro Techniques; D007476:Ionophores / Q000494:pharmacology; D016210:Methacholine Chloride / Q000494:pharmacology*; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D010277:Parasympathomimetics / Q000494:pharmacology*; D010306:Parotid Gland / Q000166:cytology / Q000378:metabolism*; D011817:Rabbits; D011976:Receptors, Muscarinic / Q000378:metabolism; D019284:Thapsigargin / Q000494:pharmacology; D043222:Ubiquitin Thiolesterase / Q000378:metabolism
D004791:Enzyme Inhibitors; D006454:Hemoglobins; D007476:Ionophores; D020030:Nitric Oxide Donors; D010277:Parasympathomimetics; D011976:Receptors, Muscarinic; D016210:Methacholine Chloride; D009599:Nitroprusside; D009569:Nitric Oxide; D000001:Calcimycin; D019284:Thapsigargin; D000681:Amylases; D043222:Ubiquitin Thiolesterase; D019331:NG-Nitroarginine Methyl Ester