Herpes Simplex Type I Virus Infection of Cultured Human Vascular Endothelial Cells: Expression of Cell Adhesion Molecules and Induction of Interferon and Cytokine Production by Blood Mononuclear Cells.
The expression of cell adhesion molecules, P- and E-selectins, ICAM-1, and VCAM-1, was studied in cultured human vascular endothelial cells (ECs) infected by herpes simplex type I virus (HSV-1). It was shown that ECs without any signs of the cytopathogenic effect (CPE) expressed on their surface P- and E-selectins as soon as 24 h after infection. No appearance of VCAM-1 or increase in ICAM-1 expression was detected. Peripheral blood mononuclear cells (PBMCs) isolated by gradient centrifugation adhered preferentially with HSV-1-infected morphologically unchanged ECs but not with cells modified in result of CPE. The interferon and cytokine production by PBMCs was assayed after their contact with infected and glutaraldehyde-fixed ECs. The secretion of IFN-alpha, IFN-gamma, IL-1, IL-6, and TNF-alpha (but not of IL-4) was found to be inducible and correlated with the multiplicity of infection. Obtained results allow to consider a described cell culture system as a model for further investigation of initial stages of HSV-1 infection and of pathogenesis of vascular disease.
Scheglovitova
Olga N.
ON
Gamaleya Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences, Moscow, Russia.
Romanov
Yuri A.
YA
Maksianina
Ekaterina V.
EV
Kabaeva
Nayle V.
NV
Pronin
Andrey G.
AG