title: Low T cell receptor expression and thermal fluctuations contribute to formation of dynamic multifocal synapses in thymocytes.
abstract: Mature T cell activation and selection of immature T cells (thymocytes) are both initiated by binding of T cell receptor (TCR) molecules on the surface of T cells to MHC peptide (MHCp) molecules on the surface of antigen-presenting cells. Recent experiments have shown that the spatial pattern of receptors and ligands in the intercellular junction (synapse) is different during thymocyte selection compared with mature T cell activation. Using a statistical mechanical model, we show that lower TCR expression in thymocytes contributes to effecting these differences. An analogy with the phase behavior of simple fluids helps clarify how, for low TCR expression, thermal fluctuations lead to the dynamic synapse patterns observed for thymocytes. We suggest that a different synapse pattern resulting from lower TCR expression, which could mediate differential signaling, may be the reason why TCR expression level is low in thymocytes.
authors.affiliation: Biophysics Graduate Group, University of California, Berkeley, CA 94720, USA.
authors.forename: Sung-Joo E
authors.initials: SJ
authors.lastname: Lee
authors.forename: Yuko
authors.initials: Y
authors.lastname: Hori
authors.forename: Arup K
authors.initials: AK
authors.lastname: Chakraborty
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002462:Cell Membrane / Q000276:immunology; D066298:In Vitro Techniques; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007365:Intercellular Junctions / Q000276:immunology; D008024:Ligands; D008213:Lymphocyte Activation; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism; D051379:Mice; D018715:Microscopy, Video; D018448:Models, Immunological; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism*; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology*; D013816:Thermodynamics
chemical_list: D008024:Ligands; D016169:Lymphocyte Function-Associated Antigen-1; D011948:Receptors, Antigen, T-Cell; D018799:Intercellular Adhesion Molecule-1