title: [The method for determination of aconitum alkaloids in Radix Aconiti Kusnezoffii].
abstract: The methods for the determination of Aconitum alkaloids in Radix Aconiti Kusnezoffii were established. Volumetric analysis with the indicator of methyl red-bromcresol green and colorimetric analysis with acid dye of bromecresol green under the wavelength of 416 nm were used for the assay of total alkaloids. Colorimetric analysis of hydroxamic acid-Fe was for the assay of total ester-diterpene type Aconitum alkaloids. Three kinds of diester-diterpene type Aconitum alkaloids (aconitine, hypaconitine and mesaconitine) were determined by HPLC method. The HPLC system consisted of C18 as the stationary phase and acetonitrile: buffer (acetic acid-triethylamine, pH 6.25) = 71:29 as the mobile phase. The wavelength was 235 nm.
authors.affiliation: School of Pharmacy, Fudan University, Shanghai 200032.
authors.forename: Jianming
authors.initials: J
authors.lastname: Huang
authors.forename: Jixian
authors.initials: J
authors.lastname: Guo
authors.forename: Mingming
authors.initials: M
authors.lastname: Sun
authors.forename: Bin
authors.initials: B
authors.lastname: Lu
authors.forename: Gengli
authors.initials: G
authors.lastname: Duan
authors.forename: Wansheng
authors.initials: W
authors.lastname: Chen
mesh_terms: D000157:Aconitine / Q000031:analogs & derivatives* / Q000032:analysis*; D031841:Aconitum / Q000737:chemistry*; D000470:Alkaloids / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003124:Colorimetry / Q000379:methods; D004396:Coloring Agents; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D010598:Pharmacognosy
chemical_list: D000470:Alkaloids; D004396:Coloring Agents; D004365:Drugs, Chinese Herbal; C058309:hypaconitine; C019470:mesaconitine; D000157:Aconitine