title: Human Oct-1L isoform has tissue-specific expression pattern similar to Oct-2.
abstract: POU homeodomain proteins are important regulators of ubiquitous as well as tissue-specific transcription. Ubiquitously expressed Oct-1 and tissue-specific Oct-2 proteins are members of the POU family and contain very similar DNA-binding POU domains. While Oct-1 is ubiquitous, Oct-2 is predominantly expressed in B cells, in activated T cells and in nervous system. Oct-1 is involved in regulation of some houskeeping genes-histone H2B, snRNAs as well as in tissue-specific regulation of immunoglobuline gene transcription and of some other genes. Here we report that novel alternatively spliced product of the human Oct-1 gene encode Oct-1L isoform with tissue-specific expression pattern, similar to Oct-2. Oct-1L differ from ubiquitously expressed Oct-1A in 5'-terminal exon (exon 1L). Analysis of nucleotide sequences from Human Genome Data Bank has located exon 1L about 108 kbp downstream ubiquitously expressed exon 1U. Amino terminus of Oct-1L show extensive similarity to amino terminus of Oct-2. We suppose, that Oct-1L may has a specific role in gene expression in lymphoid tissues and brain.
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov Str., 32, 119991, Moscow, Russia.
authors.forename: Nadejda N
authors.initials: NN
authors.lastname: Luchina
authors.forename: Ivan V
authors.initials: IV
authors.lastname: Krivega
authors.forename: Elizaveta V
authors.initials: EV
authors.lastname: Pankratova
mesh_terms: D017398:Alternative Splicing / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D020869:Gene Expression Profiling; D051863:Host Cell Factor C1; D006801:Humans; D019169:Jurkat Cells; D050811:Octamer Transcription Factor-1; D050813:Octamer Transcription Factor-2; D009928:Organ Specificity; D020033:Protein Isoforms / Q000235:genetics; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; C497692:HCFC1 protein, human; D051863:Host Cell Factor C1; D050811:Octamer Transcription Factor-1; D050813:Octamer Transcription Factor-2; C494165:POU2F1 protein, human; C058435:POU2F2 protein, human; D020033:Protein Isoforms; D014157:Transcription Factors