title: Apolipoprotein E (apoE) uptake and distribution in mammalian cell lines is dependent upon source of apoE and can be monitored in living cells.
abstract: As part of investigations of the cellular uptake of apolipoprotein E (apoE) relevant to Alzheimer's disease we have found that different preparations of apoE are handled differently by cells expressing the LDL-receptor. Comparing recombinant, cellular and native apoE, complexed with different preparations of lipid we find that only cellular and native apoE enter a vesicular compartment. Some, but not all of these apoE containing vesicles are lysosomes. In order to further examine the intracellular fate of apoE we demonstrate that apoE-Enhanced green fluorescent protein chimeric protein can be taken up from medium by recipient cells and tracked within these cells for extended periods.
authors.affiliation: Department of Neuroscience, Institute of Psychiatry, King's College London, Denmark Hill De Crespigny Park, London SE5 8AF, UK.
authors.forename: M C
authors.initials: MC
authors.lastname: Ljungberg
authors.forename: A
authors.initials: A
authors.lastname: Asuni
authors.forename: J
authors.initials: J
authors.lastname: Pearce
authors.forename: R
authors.initials: R
authors.lastname: Dayanandan
authors.forename: W
authors.initials: W
authors.lastname: März
authors.forename: M M
authors.initials: MM
authors.lastname: Hoffmann
authors.forename: P
authors.initials: P
authors.lastname: Bertrand
authors.forename: G
authors.initials: G
authors.lastname: Siest
authors.forename: H T R
authors.initials: HT
authors.lastname: Rupniak
authors.forename: B H
authors.initials: BH
authors.lastname: Anderton
authors.forename: M
authors.initials: M
authors.lastname: Huettinger
authors.forename: S
authors.initials: S
authors.lastname: Lovestone
mesh_terms: D000818:Animals; D001057:Apolipoproteins E / Q000378:metabolism*; D019556:COS Cells; D002460:Cell Line; D002522:Chlorocebus aethiops; D004926:Escherichia coli; D006801:Humans; D051379:Mice; D020033:Protein Isoforms / Q000378:metabolism; D011817:Rabbits; D011994:Recombinant Proteins / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000378:metabolism
chemical_list: D001057:Apolipoproteins E; D020033:Protein Isoforms; D011994:Recombinant Proteins