Arrays of mobile tethered vesicles on supported lipid bilayers.
We report a new system of laterally mobile, arrayed vesicles that are encoded with DNA to control tethering to fluid-supported phospholipid bilayers. The motion of individual fluorescently labeled vesicles, specifically bound, are easily visualized by fluorescence video microscopy and observed to collide reversibly on the surface. This system is an ideal model for studying interactions involving membranes, in particular integral membrane proteins.
Yoshina-Ishii
Chiaki
C
Department of Chemistry, Stanford University, California 94305-5080, USA.
Boxer
Steven G
SG
D005456:Fluorescent Dyes / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D016376:Oligonucleotides, Antisense / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry
D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D008081:Liposomes; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; D010713:Phosphatidylcholines