The effects of UV waveband and cis-urocanic acid on tumour outgrowth in mice.
Immunogenic murine tumours that are normally rejected upon transplantation into syngeneic hosts grow progressively if the hosts are UV-irradiated prior to tumour implantation. Using three sources of UV we investigated the most effective waveband and dose for increased outgrowth of injected fibrosarcoma (FSA) cells in mice, compared with unirradiated controls. The animals were exposed on their shaved dorsal skin twice a week for 3 weeks to either broad-band (TL12) or narrow-band (TL01) UVB, or UVA-I radiation; FSA cells were then injected subcutaneously into the UV-irradiated skin. Exposure to TL12 at doses higher than 1000 J m(-2), but not TL01 or UVA-I, caused increased outgrowth of the injected tumour cells. The effect of the UV irradiation seemed to be local as injection of the FSA cells into the unirradiated ventral skin of mice irradiated with TL12 on the dorsal surface did not result in increased outgrowth of the tumours. Cis-urocanic acid, a recognised initiator of UV-induced immunosuppression, applied topically or intradermally for 3 weeks prior to FSA cell injection, had no effect on the rate of tumour outgrowth. Similarly, injection of a monoclonal antibody with specificity for cis-urocanic acid, prior to each irradiation with TL12, did not reverse the increased growth of FSA cells injected into the UV-irradiated skin. Thus wavelengths within the broad-band UVB range are the most effective for inducing increased outgrowth of FSA cells; cis-urocanic acid is not an important mediator in this UV-enhanced growth of tumours.
Macve
Joanna C
JC
Medical Microbiology, University of Edinburgh Medical School, Edinburgh, Scotland EH8 9AG.
Norval
Mary
M
D000818:Animals; D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005354:Fibrosarcoma / Q000139:chemically induced / Q000209:etiology* / Q000276:immunology* / Q000473:pathology; D007536:Isomerism; D051379:Mice; D008809:Mice, Inbred C3H; D009381:Neoplasms, Radiation-Induced / Q000276:immunology* / Q000473:pathology; D012867:Skin / Q000276:immunology / Q000528:radiation effects; D013997:Time Factors; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays / Q000009:adverse effects*; D014560:Urocanic Acid / Q000009:adverse effects* / Q000737:chemistry
D014560:Urocanic Acid