title: A new approach for evaluating antigen-specific T cell responses to myelin antigens during the course of multiple sclerosis.
abstract: We used a flow cytometry assay to measure proliferation and cytokine production of self-antigen-specific T cells in individual patients during the clinical course of multiple sclerosis (MS). Myelin-associated oligodendrocytic basic protein (MOBP) was selected for proof of principles in the assay, along with myelin basic protein (MBP) to assess specific activated T cells in 10 MS patients over an 18-month period, in parallel with brain magnetic resonance imaging (MRI) scans and clinical rating scale. A positive correlation occurred between antigen-specific T cell proliferation and interferon-gamma production with clinical relapses and MRI lesion activity that was absent when the same patients were in remission.
authors.affiliation: Division of Virology, Department of Neuropharmacology, The Scripps Research Institute (IMM-6), 10550 North Torrey Pines Road, La Jolla, CA 92037, USA. nathaliearbour@hotmail.com
authors.forename: Nathalie
authors.initials: N
authors.lastname: Arbour
authors.forename: Andreas
authors.initials: A
authors.lastname: Holz
authors.forename: Jack C
authors.initials: JC
authors.lastname: Sipe
authors.forename: Denise
authors.initials: D
authors.lastname: Naniche
authors.forename: John S
authors.initials: JS
authors.lastname: Romine
authors.forename: Jack
authors.initials: J
authors.lastname: Zyroff
authors.forename: Michael B A
authors.initials: MB
authors.lastname: Oldstone
mesh_terms: D000328:Adult; D001324:Autoantigens / Q000097:blood / Q000276:immunology*; D001921:Brain / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000097:blood / Q000276:immunology* / Q000473:pathology; D004676:Myelin Basic Protein / Q000097:blood / Q000276:immunology*; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein / Q000097:blood / Q000276:immunology*; D063308:Myelin-Oligodendrocyte Glycoprotein; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism
chemical_list: D001324:Autoantigens; D018984:Epitopes, T-Lymphocyte; C560487:MOBP protein, human; C571326:MOG protein, human; D004676:Myelin Basic Protein; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D063308:Myelin-Oligodendrocyte Glycoprotein