[Corneal preservation at 4 degrees C--experimental study].
OBJECTIVE
To evaluate the 4 degrees C method of corneal preservation (in moist chamber or in preservation media To determine the duration of prevention.


METHODS
Preservation in moist chamber at 4 degrees C, group of 10 pig eyes. Preservation: the entire globe at 4 degrees C examination: direct specular microscopy with a Tomey device, daily, for 4 days. Preservation in Likorol DX at 4 degrees C, group: 20 pig eyes Preservation: Likorol DX medium, we change the medium after 7 days. examinations: inverse specular microscopy, with a Zeiss device, after 1, 4, 7, 10 and 14 days.


RESULTS
Preservation in moist chamber at 4 degrees C. The average endothelial cell loss was 13.2% after a day of preservation and 27.1% after 2 days. Preservation in Likorol DX at 4 degrees C The average endothelial cell loss was 2.8% after a day of preservation, 13.8% after 7 days and 22.6% after 10 days.


CONCLUSIONS
The corneal preservation in moist chamber at 4 degrees C is a useful method; it is safe to preserve the globe only for 24 hours. It is safe to preserve corneas in Likorol DX medium for 7 days, there is an endothelial cell loss, about 13.8%.
Zemba
M
M
Rancea
Claudia
C
Bobeico
Veronica
V
Ciuca
Corina
C
Bratulescu
M
M
Mazilu
Monica
M
D000818:Animals; D002809:Chondroitin Sulfates; D003315:Cornea; D015925:Cryopreservation / Q000379:methods*; D004728:Endothelium, Corneal; D019188:Organ Preservation Solutions; D015203:Reproducibility of Results; D013552:Swine; D013997:Time Factors
D019188:Organ Preservation Solutions; D002809:Chondroitin Sulfates