title: [Correlation between epidermal growth factor and overexpression of vascular endothelial growth factor in hepatocellular carcinoma].
abstract: OBJECTIVE
  To investigate the correlation between epidermal growth factor (EGF) and overexpression of vascular endothelial growth factor (VEGF) in hepatocellular carcinoma (HCC).
  
  
  METHODS
  Expressions of EGF, VEGF and microvessel density were studied through immunohistochemical SABC method in 36 HCC specimens, their paraneoplastic liver tissues and 6 normal liver tissues with the correlation between these parameters analyzed. Recombinant human EGF was used to stimulate HepG(2) cells and semi-quantitative reverse transcription PCR was adopted to detect the expression of VEGF in HepG(2) cells.
  
  
  RESULTS
  The positive rates of EGF and VEGF expression in HCC tissue were 75.0% and 88.9%. There was positive correlation between EGF and VEGF expression (P < 0.01, r = 0.462). Recombinant human EGF could induce the expression of VEGF in HepG(2) cells in a dose and time dependent manner.
  
  
  CONCLUSIONS
  The expression of EGF in HCC underlies the overexpression of VEGF in HCC.
authors.affiliation: Department of General Surgery, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Gengwen
authors.initials: G
authors.lastname: Huang
authors.forename: Lianyue
authors.initials: L
authors.lastname: Yang
authors.forename: Jianqing
authors.initials: J
authors.lastname: Yang
authors.forename: Heli
authors.initials: H
authors.lastname: Liu
authors.forename: Zhili
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D016228:Endothelial Growth Factors / Q000032:analysis*; D004815:Epidermal Growth Factor / Q000032:analysis*; D005260:Female; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis*; D008113:Liver Neoplasms / Q000378:metabolism*; D008222:Lymphokines / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D009389:Neovascularization, Pathologic; D013223:Statistics as Topic; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
chemical_list: D016228:Endothelial Growth Factors; D036341:Intercellular Signaling Peptides and Proteins; D008222:Lymphokines; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D004815:Epidermal Growth Factor