title: Determination of a minimal region of loss of heterozygosity on chromosome 18q21.33 in osteosarcoma.
abstract: Previous analysis of tumor-specific constitutional LOH had identified a putative tumor-suppressor gene (LOH18CR) active in osteosarcoma tumorigenesis, which mapped to a subregion of chromosome 18q linked to both familial Paget's disease and FEO. Using 9 new polymorphic loci within the previous minimal region of LOH, we have reduced the minimal region of LOH in osteosarcoma tumors to localize the LOH18CR locus to the distal end of chromosome 18q21.33. This new region is approximately 500 kb and contains at least 7 known genes; however, it excludes 2 previous candidate genes: TNFRSF11A (RANK) and BCL2.
authors.affiliation: Department of Pediatrics, University of Texas Health Science Center, San Antonio, TX, USA.
authors.forename: Teresa L
authors.initials: TL
authors.lastname: Johnson-Pais
authors.forename: Maggie J
authors.initials: MJ
authors.lastname: Nellissery
authors.forename: Donald G
authors.initials: DG
authors.lastname: Ammerman
authors.forename: Dharmini
authors.initials: D
authors.lastname: Pathmanathan
authors.forename: Pardeep
authors.initials: P
authors.lastname: Bhatia
authors.forename: Carolyn L
authors.initials: CL
authors.lastname: Buller
authors.forename: Robin J
authors.initials: RJ
authors.lastname: Leach
authors.forename: Marc F
authors.initials: MF
authors.lastname: Hansen
mesh_terms: D001859:Bone Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology; D002874:Chromosome Mapping; D002887:Chromosomes, Human, Pair 18 / Q000235:genetics*; D004247:DNA / Q000032:analysis / Q000235:genetics; D017931:DNA Primers; D016147:Genes, Tumor Suppressor; D005819:Genetic Markers; D006023:Glycoproteins / Q000235:genetics; D006720:Homozygote; D006801:Humans; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats; D053244:Osteoprotegerin; D012516:Osteosarcoma / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology; D016133:Polymerase Chain Reaction; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics; D018124:Receptors, Tumor Necrosis Factor; D012307:Risk Factors
chemical_list: D017931:DNA Primers; D005819:Genetic Markers; D006023:Glycoproteins; D053244:Osteoprotegerin; D019253:Proto-Oncogene Proteins c-bcl-2; D018160:Receptors, Cytoplasmic and Nuclear; D018124:Receptors, Tumor Necrosis Factor; C506030:TNFRSF11B protein, human; D004247:DNA