title: Solution structure of MT_nc, a novel metallothionein from the Antarctic fish Notothenia coriiceps.
abstract: The structure of [113Cd(7)]-metallothionein (MT_nc) of the Antarctic fish Notothenia coriiceps, the first three-dimensional structure of a fish metallothionein, was determined by homonuclear 1H NMR experiments and heteronuclear [1H, 113Cd]-correlation spectroscopy. MT_nc is composed of an N-terminal beta domain with 9 cysteines and 3 metal ions and a carboxy-terminal alpha-domain with 11 cysteines and 4 metal ions. The position of the ninth Cys of the alpha domain of MT_nc is different from the corresponding Cys of mammalian MTs. As a result, the last CXCC motif in the mammalian MT sequence becomes CXXXCC in the fish MT. This difference leads to a structural change of the alpha domain and, in turn, to a different charge distribution with respect to that observed in mammalian metallothioneins.
authors.affiliation: CNR, Institute of Protein Biochemistry, via Marconi 10, I 80125, Naples, Italy.
authors.forename: Clemente
authors.initials: C
authors.lastname: Capasso
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Carginale
authors.forename: Orlando
authors.initials: O
authors.lastname: Crescenzi
authors.forename: Daniela
authors.initials: D
authors.lastname: Di Maro
authors.forename: Elio
authors.initials: E
authors.lastname: Parisi
authors.forename: Roberta
authors.initials: R
authors.lastname: Spadaccini
authors.forename: Piero Andrea
authors.initials: PA
authors.lastname: Temussi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000864:Antarctic Regions; D005399:Fishes / Q000378:metabolism*; D008668:Metallothionein / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment
chemical_list: D008668:Metallothionein