GUTI: a new antigen in the Cromer blood group system.
BACKGROUND
The Cromer blood group system consists of seven high-incidence and three low-incidence antigens carried on decay-accelerating factor (DAF). This report describes the identification and characterization of a new Cromer high-incidence antigen, named GUTI.


METHODS
RT-PCR and sequence analysis were performed on cDNA prepared from a Chilean donor whose serum contained the alloantibody (anti-GUTI). Based on the observed point mutation, a PCR-RFLP assay using MaeII was developed. To map the epitope, DAF-deletion mutants were tested by immunoblotting with anti-GUTI.


RESULTS
Sequence analysis revealed a substitution of 719G>A in DAF in the proband. The proband's parents and two daughters were heterozygotes for 719G>A, one sister whose RBCs typed GUTI- was homozygous for 719A, and one sister had the wild-type DAF (719G). Seven additional heterozygote samples were identified among 214 Chileans. No heterozygotes were found in 197 New York donors. Analysis using DAF-deletion mutants showed the antigenic determinant to be within short consensus repeat (SCR) 4.


CONCLUSIONS
This study describes a novel high- incidence antigen (GUTI) in the Cromer blood group system characterized by the amino acid arginine at position 206 in SCR4 of DAF. The GUTI-negative proband has a substitution mutation that predicts for histidine at this position.
Storry
Jill R
JR
Immunohematology Laboratory, New York Blood Center, New York, NY 10021, USA.
Sausais
Laima
L
Hue-Roye
Kim
K
Mudiwa
Flora
F
Ferrer
Zennie
Z
Blajchman
Morris A
MA
Lublin
Douglas M
DM
Ma
Bei-Wen
BW
Miquel
Juan F
JF
Nervi
Flavio
F
Pereira
Jaime
J
Reid
Marion E
ME
D000328:Adult; D001789:Blood Group Antigens / Q000276:immunology*; D018958:CD55 Antigens / Q000276:immunology*; D002677:Chile; D018076:DNA, Complementary / Q000737:chemistry; D015252:Deoxyribonucleases, Type II Site-Specific; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000276:immunology; D017353:Gene Deletion; D006801:Humans; D007518:Isoantibodies / Q000097:blood; D007519:Isoantigens / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D008297:Male; D009154:Mutation; D012150:Polymorphism, Restriction Fragment Length; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D001789:Blood Group Antigens; D018958:CD55 Antigens; D018076:DNA, Complementary; C473225:GUTI antigen, human; D007518:Isoantibodies; D007519:Isoantigens; C040837:endodeoxyribonuclease MaeII; D015252:Deoxyribonucleases, Type II Site-Specific