title: [Establishment of a double-antigen sandwich ELISA for detecting total antibodies to human immunodeficiency virus type 1/2].
abstract: OBJECTIVE
  To describe and evaluate a double-antigen sandwich ELISA for detecting human immunodeficiency virus type 1/2 (HIV-1/2) specific antibodies.
  
  
  METHODS
  The peptides gp41.1(sp1), gp41.2(sp2), gp120(sp3) and p24(sp4) of HIV-1 and gp36(sp5) of HIV-2 were artificially synthesized. Then sp1, sp3, sp4 and sp5 were used as coating antigens; sp1, sp2, sp4 and sp5 labeled with HRP were used as conjugates in this sandwich ELISA.
  
  
  RESULTS
  The specificity and sensitivity of the assay were both 100% in detecting anti-HIV of 40 control sera of the second generation panel, higher than indirect ELISA (specificity 90% and sensitivity, 65%, respectively). All of 210 sera from individuals with other diseases were negative for anti-HIV. The consistency rate was 100% when our sandwich ELISA and Abbott HIVAB were used to detect anti-HIV in 90 healthy blood donors and 88 HIV infected individuals.
  
  
  CONCLUSIONS
  The results showed that this sandwich ELISA for detection of anti-HIV is specific, sensitive and convenient, and it is suitable for screening blood donors and detecting HIV infection.
authors.affiliation: Department of Virology, The 302nd Hospital of PLA, Beijing 100039, China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: He
authors.forename: Panyong
authors.initials: P
authors.lastname: Mao
authors.forename: Jun
authors.initials: J
authors.lastname: Hou
authors.forename: Shiwen
authors.initials: S
authors.lastname: Hong
authors.forename: Lei
authors.initials: L
authors.lastname: Zhu
authors.forename: Yan
authors.initials: Y
authors.lastname: Hu
authors.forename: Yanping
authors.initials: Y
authors.lastname: Bai
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D015483:HIV Antibodies / Q000097:blood*; D015658:HIV Infections / Q000097:blood / Q000821:virology*; D015497:HIV-1 / Q000276:immunology; D015498:HIV-2 / Q000276:immunology; D006801:Humans
chemical_list: D015483:HIV Antibodies