Interleukin-6 receptor expression and localization after transient global ischemia in gerbil hippocampus.
Ischemia results in increased interleukin-6 (IL-6) expression in the brain. To prove a connection between IL-6 upregulation and IL-6 receptor (IL-6R) expression following ischemia, we analyzed cell-type specific expression changes of IL-6R using transient global ischemia in the gerbil as a model. In sham operated animals, IL-6R mRNA and protein were mainly detected in hippocampal pyramidal cells and interneurons. After ischemia, IL-6R was expressed in neurons but there was no increase during the peak IL-6 expression. Neuronal IL-6R mRNA and protein decreased in parallel with pyramidal cell death, starting 2 days after ischemia. Double-labeling experiments revealed that in postischemic hippocampus IL-6R was not present in GFAP-reactive astrocytes but that the surviving parvalbumin containing interneurons expressed IL-6R mRNA.
Vollenweider
Florence
F
Institute of Anatomy, Basel University, Pestalozzistrasse 20, CH-4056 Basel, Switzerland. florence.vollenweider@unibas.ch
Herrmann
Martina
M
Otten
Uwe
U
Nitsch
Cordula
C
D000818:Animals; D005786:Gene Expression Regulation / Q000502:physiology; D005849:Gerbillinae; D006624:Hippocampus / Q000378:metabolism*; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis; D019947:Receptors, Interleukin-6 / Q000096:biosynthesis*
D012333:RNA, Messenger; D019947:Receptors, Interleukin-6