Inhibition of peritoneal metastasis in an animal gastric cancer model by interferon-gamma and interleukin-2.
BACKGROUND
The anti-tumor activity of interferon-gamma (IFN-gamma) and interleukin-2 (IL-2) have been shown in human gastric cancer cell lines, however, their therapeutic effects on peritoneal metastasis have not been investigated.


METHODS
The anti-tumor activity of IFN-gamma and IL-2 against the gastric cancer cell line, MKN45, was evaluated by in vitro antiproliferation assays and in animal-tumor models of intraperitoneal (i.p.) and subcutaneous (s.c.) tumors.


RESULTS
Survival of the animals with i.p. tumor was longest in those given IFN-gamma, followed by those given IL-2, IL-2 + IFN-gamma and saline (control) with median survival of 88 +/- 27 days, 73 +/- 30 days, 53 +/- 20 days and 32 +/- 1.1 days, respectively (p < 0.001 vs control). In animals with s.c. tumor, IFN-gamma significantly inhibited tumor growth as compared with the control. In vitro, IFN-gamma alone inhibited the growth of MKN45 and induced apoptosis, while IL-2 had no additional effect.


CONCLUSIONS
IFN-gamma inhibited the growth of gastric cancer cells in vitro and in a peritoneal tumor model. The therapeutic effects were partially antagonized by IL-2.
Okamoto
Takahide
T
First Department of Surgery, Yokohama City University, School of Medicine, Kanazawa-ku, Yokohama, Japan.
Tsuburaya
Akira
A
Yanoma
Syunsuke
S
Yoshikawa
Takaki
T
Cho
Haruhiko
H
Takanashi
Yoshinori
Y
Noguchi
Yoshikazu
Y
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D006801:Humans; D007371:Interferon-gamma / Q000008:administration & dosage / Q000494:pharmacology*; D007376:Interleukin-2 / Q000008:administration & dosage / Q000494:pharmacology*; D051379:Mice; D008813:Mice, Inbred ICR; D008819:Mice, Nude; D010534:Peritoneal Neoplasms / Q000517:prevention & control* / Q000556:secondary*; D011897:Random Allocation; D011994:Recombinant Proteins; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000473:pathology; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
D000970:Antineoplastic Agents; D007376:Interleukin-2; D011994:Recombinant Proteins; D007371:Interferon-gamma