Restricted expression of membrane type 1-matrix metalloproteinase by myofibroblasts adjacent to human breast cancer cells.
The membrane type-1 matrix metalloproteinase (MT1-MMP), a protease originally identified in breast carcinoma, is characterized by its capacity to activate other MMPs (MMP-2 and MMP-13) and to degrade extracellular matrix. Our study was undertaken to localize and identify the MT1-MMP expressing cells in human breast adenocarcinomas. A textural analysis of images obtained by immunohistochemistry and in situ hybridization showed precisely the co-expression of alpha smooth muscle actin (alphaSM actin) and MT1-MMP in myofibroblasts. MT1-MMP expression is confined to myofibroblasts in close contact with tumor cells. In sharp contrast, the expression of MMP-2 was more widely distributed in both alphaSM actin positive and negative cells close to and at distance from cancer cell clusters. Our in vitro observations are consistent with the higher level of MT1-MMP expression and of MMP-2 activation observed in alphaSM actin positive fibroblasts derived from breast tumors, as compared to normal breast fibroblasts. Collectively, these results implicate myofibroblasts as major producer of MT1-MMP in breast cancer and emphasize the importance of stromal-epithelial cell interactions in their progression.
Bisson
Christèle
C
Laboratory of Tumor and Development Biology, University of Liège, Liège, Belgium.
Blacher
Silvia
S
Polette
Myriam
M
Blanc
Jean-Frédéric
JF
Kebers
Florence
F
Desreux
Joëlle
J
Tetu
Bernard
B
Rosenbaum
Jean
J
Foidart
Jean-Michel
JM
Birembaut
Philippe
P
Noel
Agnès
A
D000199:Actins / Q000096:biosynthesis; D000230:Adenocarcinoma / Q000201:enzymology / Q000378:metabolism; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000201:enzymology / Q000378:metabolism*; D017364:Collagenases / Q000378:metabolism; D017077:Culture Media, Conditioned / Q000494:pharmacology; D018450:Disease Progression; D004789:Enzyme Activation; D004847:Epithelial Cells / Q000166:cytology; D005347:Fibroblasts / Q000201:enzymology* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism*; D053507:Matrix Metalloproteinase 11; D053509:Matrix Metalloproteinase 13; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D008666:Metalloendopeptidases / Q000378:metabolism; D008856:Microscopy, Fluorescence; D009130:Muscle, Smooth / Q000378:metabolism; D009369:Neoplasms / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D017154:Stromal Cells / Q000166:cytology; D014407:Tumor Cells, Cultured
D000199:Actins; D017077:Culture Media, Conditioned; D012333:RNA, Messenger; D017364:Collagenases; C507079:MMP13 protein, human; D053507:Matrix Metalloproteinase 11; D053509:Matrix Metalloproteinase 13; D008666:Metalloendopeptidases; D020778:Matrix Metalloproteinase 2; D020781:Matrix Metalloproteinase 1