title: Probing isomeric differences of phosphorylated carbohydrates through the use of ion/molecule reactions and FT-ICR MS.
abstract: Through the use of ion/molecule reactions and tandem mass spectrometry, phosphate position is assigned in both phosphorylated monosaccharides and oligosaccharides. In previous work phosphate moieties of monosaccharides were stabilized under collisional activation, by first derivatizing the deprotonated monosaccharide with trimethyl borate through an ion/molecule reaction, and the phosphate position determined through marker ions generated in tandem mass spectra. In this work, the methodology is extended to larger phosphorylated oligomers employing chlorotrimethylsilane (TMSCl) as the ion/molecule reagent. Phosphorylated monosaccharides were first investigated to determine diagnostic ions for phosphate linkage in monomeric standards. It was observed that the diagnostic ions showed both linkage and some monosaccharide stereochemical information. Furthermore, it was observed that TMS addition stabilized the phosphate moiety under collisionally activated conditions. Upon identification of the diagnostic ions, the methodology was applied to lactose-1-phosphate. It was found that TMSCl, stabilized the phosphate moiety upon collisional activation, and furthermore, the phosphate linkage could be determined through tandem mass spectrometric analysis. As a further extrapolation to biologically relevant problems, the methodology was applied to a lipophosphoglycan analog from the protozoan parasite Leishmania. This sample contains bridging phosphates which were converted to terminal phosphates through collision induced dissociation. The sample was then analyzed in the same manner as lactose-1-phosphate, yielding phosphate linkage information and stereochemical information. This study showed that, using the developed methodology, phosphate linkage can be determined from both monosaccharides and larger oligosaccharides; furthermore it is applicable to samples in which the phosphates are either terminating or bridging.
authors.affiliation: Department of Chemistry, University of California at Berkeley, 94720, USA.
authors.forename: M D
authors.initials: MD
authors.lastname: Leavell
authors.forename: Julie A
authors.initials: JA
authors.lastname: Leary
mesh_terms: D000818:Animals; D002241:Carbohydrates / Q000737:chemistry*; D004187:Disaccharides / Q000032:analysis; D005583:Fourier Analysis; D006028:Glycosphingolipids / Q000737:chemistry; D007477:Ions / Q000737:chemistry; D007536:Isomerism; D007891:Leishmania / Q000737:chemistry; D015394:Molecular Structure; D009005:Monosaccharides / Q000032:analysis; D010710:Phosphates / Q000032:analysis* / Q000737:chemistry*; D010766:Phosphorylation; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013403:Sugar Phosphates / Q000032:analysis / Q000737:chemistry
chemical_list: D002241:Carbohydrates; D004187:Disaccharides; D006028:Glycosphingolipids; D007477:Ions; D009005:Monosaccharides; D010710:Phosphates; D013403:Sugar Phosphates; C008290:lipophosphonoglycan; C080750:lactose 1-phosphate