title: Acrylamide: increased concentrations in homemade food and first evidence of its variable absorption from food, variable metabolism and placental and breast milk transfer in humans.
abstract: We have developed a liquid chromatography/mass spectrometry (LC-MS/MS) assay to determine acrylamide in various body fluids. The assay also allows the reliable quantitation of acrylamide in food. In a total of 11 healthy male and female subjects, we were able to show that acrylamide from food given to humans is in fact absorbed from the gut. The half-lives determined in two male subjects were 2.2 and 7 h. Acrylamide was found in human breast milk and penetrated the human placenta (n = 3). The variability of acrylamide concentrations found in this investigation is most likely caused by variable intersubject bioavailability and metabolism. This may be an important indication that the assessment of the risk from acrylamide for the individual may be very difficult without knowing the concentrations of acrylamide in the body. This should be considered in the design of any risk assessment study or post hoc analysis of earlier studies. At this time, we suggest that pregnant women and breast-feeding mothers avoid acrylamide-containing food.
authors.affiliation: Institute for Biomedical and Pharmaceutical Research, Nürnberg-Heroldsberg, Germany. ibmp@osn.de
authors.forename: Fritz
authors.initials: F
authors.lastname: Sörgel
authors.forename: Rainer
authors.initials: R
authors.lastname: Weissenbacher
authors.forename: Martina
authors.initials: M
authors.lastname: Kinzig-Schippers
authors.forename: Annette
authors.initials: A
authors.lastname: Hofmann
authors.forename: Michael
authors.initials: M
authors.lastname: Illauer
authors.forename: Andreas
authors.initials: A
authors.lastname: Skott
authors.forename: Cornelia
authors.initials: C
authors.lastname: Landersdorfer
mesh_terms: D020106:Acrylamide / Q000032:analysis / Q000493:pharmacokinetics* / Q000652:urine; D000293:Adolescent; D000328:Adult; D002273:Carcinogens / Q000032:analysis / Q000493:pharmacokinetics*; D002853:Chromatography, Liquid; D004785:Environmental Pollutants / Q000032:analysis / Q000493:pharmacokinetics* / Q000652:urine; D005260:Female; D005504:Food Analysis; D005506:Food Contamination / Q000032:analysis; D006801:Humans; D007774:Lactation / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry; D008431:Maternal-Fetal Exchange; D008875:Middle Aged; D008895:Milk, Human / Q000737:chemistry* / Q000378:metabolism; D010477:Perfusion; D010920:Placenta / Q000737:chemistry* / Q000378:metabolism; D011247:Pregnancy; D013997:Time Factors
chemical_list: D002273:Carcinogens; D004785:Environmental Pollutants; D020106:Acrylamide