Fluorescence polarization assays in signal transduction discovery.
Fluorescence polarization (FP) has become widely employed for high throughput screening used in pharmaceutical drug discovery. Assays of important signal transduction targets are now adapted to FP. In this review we examine assays for cyclic adenosine monophosphate, phosphodiesterases, and protein kinases and phosphatases using FP competitive immunoassays and a direct enzymatic method called IMAP.
Sportsman
J Richard
JR
Molecular Devices Corporation, Sunnyvale, CA, USA. richard_sportsman@moldev.com
Daijo
Janet
J
Gaudet
Elizabeth A
EA
D000818:Animals; D002138:Calibration; D000242:Cyclic AMP / Q000032:analysis / Q000378:metabolism; D005454:Fluorescence Polarization / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000032:analysis / Q000378:metabolism; D015290:Second Messenger Systems; D015398:Signal Transduction; D000099190:MAP-Kinase-Activated Kinase 2
D000242:Cyclic AMP; D047908:Intracellular Signaling Peptides and Proteins; D017346:Protein Serine-Threonine Kinases; D000099190:MAP-Kinase-Activated Kinase 2