Biosynthesis of chloroplastidic and extrachloroplastidic terpenoids in liverwort cultured cells: 13C serine as a probe of terpene biosynthesis via mevalonate and non-mevalonate pathways.
Two terpenoid biosynthetic pathways, the mevalonate and non-mevalonate (glyceraldehyde phosphate-pyruvate) routes, were examined by feeding (13)C-labeled serines ([1-(13)C]- and [3-(13)C]-) to the cultured cells of the liverwort, Heteroscyphus planus. The labeling patterns observed in the isoprenoid unit of the biosynthetically (13)C-labeled stigmasterol corresponded to those expected from the mevalonate pathway, while those of the phytyl side chain corresponded to those from the non-mevalonate pathway. Thus, serine is a potential probe to determine the origin of terpenoid biosynthesis, in either the mevalonate or non-mevalonate pathway.
Itoh
Daisuke
D
The United Graduate School of Agricultural Science, Iwate University, Morioka 020-8550, Japan.
Kawano
Kyouhei
K
Nabeta
Kensuke
K
D000107:Acetylation; D002247:Carbon Isotopes; D002478:Cells, Cultured; D002734:Chlorophyll / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism*; D035926:Hepatophyta / Q000737:chemistry*; D006868:Hydrolysis; D008798:Mevalonic Acid / Q000378:metabolism*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010836:Phytol / Q000031:analogs & derivatives / Q000378:metabolism; D012694:Serine; D013729:Terpenes / Q000737:chemistry / Q000378:metabolism*
D002247:Carbon Isotopes; D013729:Terpenes; D002734:Chlorophyll; D010836:Phytol; D012694:Serine; D008798:Mevalonic Acid