Spatially and temporally resolved delivery of stimuli to single cells.
This Communication describes the photolysis of individual optically trapped vesicles for delivering spatially and temporally resolved stimuli to single cells. A submicrometer-sized vesicle, which encapsulates the stimuli of interest, is precisely positioned with respect to a select cell by optical trapping. Application of a single focused UV laser pulse photolyzes the trapped vesicle and releases the stimuli onto a localized region of the cell. The spatial resolution of this technique is on the order of several hundreds of nanometers, while the temporal resolution lies in the sub-microsecond range.
Sun
Bingyun
B
Department of Chemistry, University of Washington, Seattle 98195-1700, USA.
Chiu
Daniel T
DT
D000818:Animals; D016466:CHO Cells; D002462:Cell Membrane / Q000502:physiology*; D002468:Cell Physiological Phenomena; D006224:Cricetinae; D003584:Cytological Techniques / Q000379:methods*; D005456:Fluorescent Dyes; D007834:Lasers; D010782:Photolysis; D015398:Signal Transduction / Q000502:physiology*
D005456:Fluorescent Dyes