Comparison of polyvinylidene fluoride and polyether sulfone membranes in filtering viral suspensions.
Low-protein-binding membranes with a pore size of 0.22 microm are used to filter aqueous solutions containing viruses. Virus adsorption to the membranes is avoided if they are made of polyvinylidene fluoride (PVDF) or if they are made of cellulose esters saturated with beef extract. Recently, a new kind of membrane filter made of polyether sulfone (PES) has become available commercially. The manufacturers claim that such membranes allow the filtration of greater volumes of sample than those made of PVDF. We compared the filtration rate and volume that could be filtered before clogging for these two membranes. The bacteriophage and enterovirus counts were then compared in sewage after filtration through the two membranes. There were no differences in virus recovery after filtration, but PES membranes allowed a higher filtration rate and clogged more slowly. The use of PES membranes is recommended.
Mocé-Llivina
L
L
Department of Microbiology, Faculty of Biology, University of Barcelona, Diagonal 645, E-08028, Barcelona, Spain.
Jofre
J
J
Muniesa
M
M
D001435:Bacteriophages / Q000302:isolation & purification*; D004770:Enterovirus / Q000302:isolation & purification*; D005374:Filtration / Q000379:methods; D008567:Membranes, Artificial; D008851:Micropore Filters; D011108:Polymers; D011145:Polyvinyls; D012722:Sewage / Q000821:virology*; D013450:Sulfones; D014773:Virology / Q000379:methods; D005465:Fluorocarbon Polymers
D008567:Membranes, Artificial; D011108:Polymers; D011145:Polyvinyls; D012722:Sewage; D013450:Sulfones; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers; C022840:polyether sulfone