[Effects of G894T mutation in the endothelial nitric oxide synthase gene on blood pressure].
OBJECTIVE
To analyze the association between G894T (Glu298Asp) mutation at exon 7 in the endothelial nitric oxide synthase gene and essential hypertension.


METHODS
One hundred and sixteen essential hypertensives without taking hypertensive medication and 136 normotensives screened from health workers in a steel factory were selected as subjects in this study. Polymerase chain reaction (PCR) and Ban II restriction enzyme digestion were performed to detect the G894T mutation.


RESULTS
G894T mutation was significantly associated with essential hypertension. The T allele frequency in essential hypertensive group was significantly higher than that in normotensive group (16.0% versus 8.8%, P = 0.019, OR = 1.96, 95% CI: 1.14 - 3.37). The levels of systolic blood pressure and diastolic blood pressure in the G894T mutant genotypes were all significantly elevated in hypertensive, normotensive, and the total subjects (P < 0.05). After adjusting factors as age, sex, smoking, alcohol drinking, body mass index, triglyceride, serum total cholesterol, serum high density lipoprotein cholesterol by analysis of multiple covariance, significant positive effect of the G894T mutant genotypes on blood pressure in the total subjects (P < 0.01) was noticed.


CONCLUSIONS
This study suggested that the G894T mutation in the endothelial nitric oxide synthase gene might serve as a major risk factor of essential hypertension in this study population.
Jia
Chong-qi
CQ
Department of Epidemiology, College of Public Health, Shandong University, Jinan 250012, China.
Zhao
Zhong-tang
ZT
Wang
Li-hua
LH
Hao
Feng-rong
FR
Feng
Yue-qiu
YQ
Wang
Shu-mei
SM
Xu
Xiao-fei
XF
Jia
Cun-xian
CX
D000328:Adult; D000483:Alleles; D001794:Blood Pressure / Q000235:genetics; D002681:China / Q000453:epidemiology; D005091:Exons; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006801:Humans; D006973:Hypertension / Q000201:enzymology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D019001:Nitric Oxide Synthase / Q000235:genetics*; D052250:Nitric Oxide Synthase Type III; D017354:Point Mutation; D012150:Polymorphism, Restriction Fragment Length; D012307:Risk Factors
C496314:NOS3 protein, human; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III