Genetic relationship of Sinorhizobium meliloti and Sinorhizobium medicae strains isolated from Caucasian region.
Sinorhizobium meliloti and Sinorhizobium medicae are two closely related species of the genus Sinorhizobium showing a similar host range, nodulating leguminous species of the genera Medicago, Melilotus and Trigonella, but their phylogenic relationship has not been elucidated yet. In this paper we report the application of three different molecular markers, (i) RFLP of nodD genes, (ii) 16S-23S rDNA intergenic gene spacer fingerprinting and (iii) amplification fragment length polymorphism to S. meliloti and S. medicae strains isolated from the Caucasian area, which is the region of origin of the host plant Medicago. The analysis of data could suggest the origin of S. medicae strains from an ancestral S. meliloti population.
Biondi
Emanuele G
EG
Department of Animal Biology and Genetics, University of Florence, via Romana 17, I-50125 Firenze, Italy.
Pilli
Elena
E
Giuntini
Elisa
E
Roumiantseva
Marina L
ML
Andronov
Euvgenij E
EE
Onichtchouk
Olga P
OP
Kurchak
Oksana N
ON
Simarov
Boris V
BV
Dzyubenko
Nikolay I
NI
Mengoni
Alessio
A
Bazzicalupo
Marco
M
D016172:DNA Fingerprinting / Q000379:methods; D019143:Evolution, Molecular; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D012338:RNA, Ribosomal, 23S / Q000032:analysis; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods; D012426:Russia; D020579:Sinorhizobium / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016962:Sinorhizobium meliloti / Q000235:genetics* / Q000302:isolation & purification; D012988:Soil Microbiology
D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S