title: Characterisation of WE-14 in porcine ocular tissue.
abstract: WE-14 is derived from the cell-specific posttranslational processing of chromogranin A (CgA) in subpopulations of neuroendocrine cells and neurons. Region- and site-specific chromogranin A, pancreastatin and WE-14 antisera were employed to study the generation of WE-14 in porcine ocular tissues. No chromogranin A or pancreastatin immunostaining was detected in ocular tissue. Immunohistochemistry detected WE-14 immunostaining in a network of nerve fibre bundles and nerve fibres throughout the limbus, cornea, iris and ciliary body with sparse nerve fibres detected throughout the choroid and sclera. Retinal analysis detected intense WE-14 immunostaining in large ovoid cells in the ganglion cell layer with weak immunostaining in a population of small cells in the inner nuclear layer; weak immunostaining was detected within the fibre layers in the inner plexiform layer. Quantitatively, the highest WE-14 tissue concentration was recorded in aqueous retinal and corneal extracts with lower concentrations in the sclera, choroid and anterior uveal tissues. Chromatographic profiling resolved a minor chromogranin A-like immunoreactant and a predominant immunoreactant co-eluting with synthetic human WE-14. This is the first study to demonstrate that WE-14 is generated in neuronal fibres primarily innervating the anterior chamber and in select cell populations in the retina.
authors.affiliation: Centre of Ophthalmology and Vision Science, Queen's University Belfast, Institute of Clinical Science, Grosvenor Road, BT12 6BA, N Ireland, Belfast, UK. j.w.curry@qub.ac.uk
authors.forename: W J
authors.initials: WJ
authors.lastname: Curry
authors.forename: A P
authors.initials: AP
authors.lastname: McCollum
authors.forename: S
authors.initials: S
authors.lastname: Brockbank
authors.forename: T A
authors.initials: TA
authors.lastname: Gardiner
authors.forename: A G
authors.initials: AG
authors.lastname: Maule
authors.forename: A W
authors.initials: AW
authors.lastname: Stitt
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002829:Choroid / Q000737:chemistry; D019439:Chromaffin Cells / Q000737:chemistry; D053379:Chromogranin A; D002864:Chromogranins / Q000276:immunology / Q000378:metabolism; D002924:Ciliary Body / Q000737:chemistry; D003315:Cornea / Q000737:chemistry; D005123:Eye / Q000737:chemistry*; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007150:Immunohistochemistry; D007498:Iris / Q000737:chemistry; D016850:Limbus Corneae / Q000737:chemistry; D009363:Neoplasm Proteins / Q000276:immunology / Q000378:metabolism*; D009412:Nerve Fibers / Q000737:chemistry; D010187:Pancreatic Hormones / Q000276:immunology / Q000378:metabolism; D012160:Retina / Q000737:chemistry; D012590:Sclera / Q000737:chemistry; D013552:Swine; D014602:Uvea / Q000737:chemistry
chemical_list: D053379:Chromogranin A; D002864:Chromogranins; D007106:Immune Sera; D009363:Neoplasm Proteins; D010187:Pancreatic Hormones; C050836:pancreastatin; C074381:chromogranin A-derived peptide, WE-14