Establishment of modified chimeric mice using GFP bone marrow as a model for neurological disorders.
Chimeric mice stably reconstituted with bone marrow cells represent a good model for analysis of the mechanism of bone marrow cell infiltration in the brain. However, in preparing chimeric mice, irradiation of the recipient mice is necessary to kill their own bone marrow before transplantation, which induces gliosis and inflammatory response by activation of astrocytes and microglia in the brain. Here, we determined the most suitable dose of irradiation associated with the least brain damage before transplantation for reconstitution of chimeric mice, using FACS analysis. Our mouse model of 10 Gy body/5 Gy head irradiation should be useful for investigating the mechanism(s) of microglial activation in various neurological disorders such as stroke, Alzheimer's disease and Parkinson's disease.
Furuya
Tsuyoshi
T
Department of Neurology, Juntendo University School of Medicine, Tokyo, Japan.
Tanaka
Ryota
R
Urabe
Takao
T
Hayakawa
Jun
J
Migita
Makoto
M
Shimada
Takashi
T
Mizuno
Yoshikuni
Y
Mochizuki
Hideki
H
D000818:Animals; D001853:Bone Marrow / Q000378:metabolism* / Q000528:radiation effects; D001854:Bone Marrow Cells / Q000378:metabolism / Q000528:radiation effects; D016026:Bone Marrow Transplantation / Q000379:methods; D002831:Choroid Plexus / Q000378:metabolism; D004195:Disease Models, Animal; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods; D049452:Green Fluorescent Proteins; D006257:Head / Q000528:radiation effects; D008164:Luminescent Proteins / Q000378:metabolism / Q000528:radiation effects; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009422:Nervous System Diseases / Q000473:pathology*; D009830:Olfactory Bulb / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects / Q000637:transplantation; D010841:Pia Mater / Q000378:metabolism; D011828:Radiation Chimera / Q000378:metabolism; D013378:Substantia Nigra / Q000378:metabolism / Q000528:radiation effects / Q000637:transplantation
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins