title: Development and evaluation of the nuclisens basic kit NASBA for the detection of RNA from Candida species frequently resistant to antifungal drugs.
abstract: We describe a Nucleic Acid Sequence Based Amplification (NASBA) protocol to detect 6 different Candida species (Candida krusei, Candida glabrata, Candida inconspicua, Candida dubliniensis, Candida norvegensis, Candida lusitaniae) and compare it to a PCR assay. NASBA showed a sensitivity of 1 Colony Forming Unit and detected RNA from all 6 Candida species within 1 working day. All 5 patients with documented candidiasis showed identical results by both methods. This assay offers a sensitive, specific and fast possibility to detect yeast RNA.
authors.affiliation: Eberhard-Karls-Universität, Medizinische Klinik, 72076, Tübingen, Germany. juergen.loeffler@med.uni-tuebingen.de <juergen.loeffler@med.uni-tuebingen.de>
authors.forename: Juergen
authors.initials: J
authors.lastname: Loeffler
authors.forename: Christiane
authors.initials: C
authors.lastname: Dorn
authors.forename: Holger
authors.initials: H
authors.lastname: Hebart
authors.forename: Philipp
authors.initials: P
authors.lastname: Cox
authors.forename: Stella
authors.initials: S
authors.lastname: Magga
authors.forename: Hermann
authors.initials: H
authors.lastname: Einsele
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000145:classification* / Q000187:drug effects*; D002177:Candidiasis / Q000175:diagnosis / Q000188:drug therapy; D025141:Drug Resistance, Fungal; D005260:Female; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D016533:Mycological Typing Techniques; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012331:RNA, Fungal / Q000032:analysis*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
chemical_list: D000935:Antifungal Agents; D012331:RNA, Fungal; D011933:Reagent Kits, Diagnostic