Effect of stimulation of nerve regeneration on posttraumatic neuronal survival in dorsal root ganglia.
The survival of neurons is a key condition for complete posttraumatic regeneration of the peripheral nerve. In experiments on rats we studied survival capacity of different neuronal subpopulations in L(IV)-L(V) dorsal root ganglia after ligation or transection and suturing of the sciatic nerve. Experiments with nerve ligation showed that IB4+ neurons are more sensitive to the injury than NF200+ neurons. By day 90 after ligation of the sciatic nerve IB4+ neurons were virtually not detected in the dorsal root ganglia. By day 90 after nerve transection the number of surviving NF200+ and IB4+ neurons decreased by 26.1 and 21.4%, respectively, in comparison with intact animals. Treatment with xymedon, a regeneration stimulator, led to a 48.5% increase in the number of surviving NF200+ neurons by day 30 after ligation of the nerve and a 50.7% increase by day 90. The number of surviving IB4+ neurons increased more than 8-fold by this term after ligation of the nerve and drug stimulation. Xymedon had a neuroprotective effect towards both neuron subpopulations, more intensely preventing apoptosis of IB4+ neurons.
Chelyshev
Yu A
YA
Department of Histology, Cytology, and Embryology, Kazan State Medical University, Russia. chelyshev@kzn.ru
Raginov
I S
IS
D000276:Adjuvants, Immunologic / Q000627:therapeutic use; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D019771:Axotomy; D002470:Cell Survival / Q000187:drug effects*; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008026:Ligation; D008297:Male; D009416:Nerve Regeneration; D009475:Neurons, Afferent / Q000166:cytology / Q000502:physiology*; D011743:Pyrimidines / Q000627:therapeutic use; D051381:Rats; D012584:Sciatic Nerve / Q000166:cytology / Q000187:drug effects* / Q000293:injuries* / Q000503:physiopathology; D013268:Stimulation, Chemical; D013997:Time Factors
D000276:Adjuvants, Immunologic; D011743:Pyrimidines; C069705:xymedon