title: Artificial insemination with canine semen stored at a low temperature.
abstract: Cooled canine semen solutions for storage were investigated with three stock solutions: egg yolk-citrate-glycine-glucose solution, egg yolk Tris-fructose citrate solution (EYT-FC), and egg yolk sodium citrate dihydrate solution (EYCD). For the control group, the second fraction of semen was examined. Nine male beagles and 37 female (47 experimental cases) beagles for artificial insemination (AI) were used. The qualities of semen stored at 4 degrees C deteriorated earlier in the control and EYCD groups. In the other two groups, sperm motility was 60% or higher after storage for 6 days and 20% or higher after storage for 12 days. On a comparison of these two groups, the sperm motility and viability were slightly higher in the EYT-FC group. A high conception rate was obtained by AI using semen stored at a low temperature for a maximum of two days in the control group and four days in the EYT-FC group.
authors.affiliation: Department of Reproduction, Nippon Veterinary and Animal Science University, Tokyo, Japan.
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Tsutsui
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Tezuka
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Mikasa
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Sugisawa
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Kirihara
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Hori
authors.forename: Eiichi
authors.initials: E
authors.lastname: Kawakami
mesh_terms: D000818:Animals; D003080:Cold Temperature; D004285:Dogs; D004530:Egg Yolk; D005260:Female; D005298:Fertility; D005306:Fertilization; D006863:Hydrogen-Ion Concentration; D007315:Insemination, Artificial / Q000379:methods*; D008297:Male; D009997:Osmotic Pressure; D011247:Pregnancy; D012661:Semen / Q000502:physiology*; D012662:Semen Preservation; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*; D013997:Time Factors