Repair of radius defect with bone-morphogenetic-protein loaded hydroxyapatite/collagen-poly(L-lactic acid) composite.
OBJECTIVE
To explore the method to repair bone defect with bone-morphogenetic-protein loaded hydroxyapatite/collagen-poly(L-lactic acid) composite.


METHODS
18 adult beagle dogs were randomly divided into 3 groups. In Group A, bone-morphogenetic-protein (BMP) loaded hydroxyapatite/collagen-poly(L-lactic acid) (HAC-PLA) scaffold was implanted in a 2 cm diaphyseal defect in the radius. In Group B, unloaded pure HAC-PLA scaffold was implanted in the defects. No material was implanted in Group C (control group). The dogs were sacrificed 6 months postoperatively. Features of biocompatibility, biodegradability and osteoinduction were evaluated with histological, radiological examinations and bone mineral density (BMD) measurements.


RESULTS
In Group A, the radius defect healed after the treatment with BMP loaded HAC-PLA. BMD at the site of the defect was higher than that of the contralateral radius. Fibrous union developed in the animals of the control group.


CONCLUSIONS
BMP not only promotes osteogenesis but also accelerates degradation of the biomaterials. Optimized design parameters of a three-dimensional porous biomaterial would give full scope to the role of BMP as an osteoinductive growth factor.
Hu
Yun-Yu
YY
Department of Orthopaedics and Traumatology, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China. zcmail2002@yahoo.com
Zhang
Chao
C
Lu
Rong
R
Xu
Jian-Qiang
JQ
Li
Dan
D
D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use*; D019485:Bone Morphogenetic Proteins / Q000627:therapeutic use*; D018786:Bone Substitutes / Q000627:therapeutic use*; D003094:Collagen / Q000627:therapeutic use*; D004285:Dogs; D017886:Durapatite / Q000627:therapeutic use*; D019344:Lactic Acid / Q000627:therapeutic use*; D008855:Microscopy, Electron, Scanning; D016348:Osseointegration; D010012:Osteogenesis; D011859:Radiography; D011884:Radius / Q000000981:diagnostic imaging / Q000473:pathology*; D014945:Wound Healing / Q000502:physiology
D001672:Biocompatible Materials; D019485:Bone Morphogenetic Proteins; D018786:Bone Substitutes; D019344:Lactic Acid; D003094:Collagen; D017886:Durapatite