title: A sensitive fluorescence optosensor for analysing propranolol in pharmaceutical preparations and a test for its control in urine in sport.
abstract: We describe a simple and selective method for analysing propranolol and a sensitive test for its control in urine. A flow-through fluorescence optosensor based on on-line immobilization in a non-ionic-exchanger (Amberlite XAD-7) solid support in a continuous flow was used in both cases. Determination was made in 5 mM H(2)PO(4)(-)/HPO(4)(2-) buffer solution at pH 6 at a working temperature of 20 degrees C. Fluorescence intensities were measured at lambda(ex/em) = 300/338 nm with a response time of 80 s, thus obtaining a linear concentration range of between 0 and 250.0 ng ml(-1) with a detection limit of 1.3 ng ml(-1), an analytical sensitivity of 6.0 ng ml(-1) and a standard deviation of 2.40% at a 150 ng ml(-1) concentration level for propranolol. We also propose a test to detect propranolol in urine with a linear concentration range between 0 and 100.0 ng ml(-1), a detection limit of 0.2 ng ml(-1), an analytical sensitivity of 1.0 ng ml(-1), and a standard deviation of 0.84% at a 75 ng ml(-1) concentration level. The effect of proteins presents in urine samples were evaluated. The two proposed methods were satisfactorily applied to commercial formulations and urine samples respectively.
authors.affiliation: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, C/Fuentenueva s/n, E-18071 Granada, Spain.
authors.forename: J F
authors.initials: JF
authors.lastname: Fernández-Sánchez
authors.forename: A Segura
authors.initials: AS
authors.lastname: Carretero
authors.forename: C
authors.initials: C
authors.lastname: Cruces-Blanco
authors.forename: A
authors.initials: A
authors.lastname: Fernández-Gutiérrez
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000032:analysis* / Q000652:urine*; D004300:Doping in Sports; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007475:Ion Exchange Resins; D011433:Propranolol / Q000032:analysis* / Q000652:urine*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods / Q000592:standards; D015813:Substance Abuse Detection / Q000379:methods* / Q000592:standards
chemical_list: D000319:Adrenergic beta-Antagonists; D007475:Ion Exchange Resins; D011433:Propranolol