Polymerase chain reaction and DNA sequencing for detection of ovine herpesvirus 2 in American bison (Bison bison).
Serum samples were collected at slaughter from 226 24-30-month-old ranch-raised, clinically normal American bison (Bison bison) bulls from North Dakota, Minnesota, Kansas, and Manitoba to assess the presence of antibodies to ovine herpesvirus 2 (OHV-2). Antibodies to OHV-2 were detected by competitive inhibition enzyme-linked immunosorbent assay in 10 of 226 (4.40%) samples. Polymerase chain reaction (PCR) analysis of sera positive for OHV-2 DNA demonstrated a 238 kilobase fragment. The nucleotide sequence of the PCR-positive samples in comparison to the reported OHV-2 nucleotide sequence resulted in a homology range of 82.8-95.4%.
Sausker
Elizabeth A
EA
Genaissance Pharmaceuticals, 5 Science Park, New Haven, CT 06511, USA.
Dyer
Neil W
NW
D000569:Americas; D000818:Animals; D016164:Bison / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D006564:Herpesviridae / Q000235:genetics* / Q000302:isolation & purification*; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012756:Sheep / Q000821:virology*; D013045:Species Specificity
D004279:DNA, Viral