Spermiogenesis in the proteocephalidean cestode Proteocephalus torulosus (Batsch, 1786).
Spermiogenesis of the proteocephalidean cestode Proteocephalus torulosus (Batsch, 1786) was examined for the first time using transmission electron microscopy. Spermiogenesis begins with the formation of a distal cytoplasmic protrusion, a differentiation zone, at the periphery of the early spermatid. This differentiation zone is lined with cortical microtubules and contains two centrioles aligned along the same axis. Subsequently, each centriole is associated with the striated root and the intercentriolar body appears between them. A flagellar bud arises from each centriole, growing later as a free flagellum. Simultaneously, a median cytoplasmic process (MCP) develops distally to the flagella. The two flagella, which are of unequal length, become longer and rotate towards the MCP. At this stage, two arching membranes appear at the base of the differentiation zone. The nucleus elongates and when both flagella are fused with the MCP, the nucleus subsequently migrates into the MCP. Finally, the advanced spermatids detach from a condensing residual cytoplasm at the level of the arching membranes.
Brunanská
M
M
Institute of Parasitology, Academy of Sciences of the Czech Republic, Branisovská 31, 370 05 Ceské Budejovice, Czech Republic. brunan@paru.cas.cz
Nebesárová
J
J
Scholz
T
T
D000818:Animals; D002589:Cestoda / Q000502:physiology* / Q000648:ultrastructure; D002590:Cestode Infections / Q000469:parasitology / Q000662:veterinary; D003530:Cyprinidae / Q000469:parasitology*; D005393:Fish Diseases / Q000469:parasitology; D007411:Intestinal Diseases, Parasitic / Q000469:parasitology / Q000662:veterinary*; D008297:Male; D008854:Microscopy, Electron; D013087:Spermatids / Q000648:ultrastructure; D013091:Spermatogenesis; D013094:Spermatozoa / Q000648:ultrastructure