The phase diagram for the ternary system propylene glycol-sodium chloride-water and their application to platelet cryopreservation.
In order to acquire freezing model of the cryopretective solution (NaCl-propylene glycol-water ternary system) for platelet, the melting points (T(f)) of this cryopretective solutions with different solute concentration and different ratio of PG mass to NaCl mass were measured by using a differential scanning calorimeter (DSC), and these experimental data were fitting by computer. An empirical equation was derived which characterized the Tf as a function of the solute concentration and the ratio of PG mass to NaCl mass inside this solution. It was concluded that the equilibrium freezing model for human platelets in this system could be used to instruct platelet cryopreserving techniques.
Liu
Jing-Han
JH
Department of Blood Transfusion, General Hospital of PLA, Centre for Clinic Blood Transfusion Medicine of PLA, Beijing, 100853, China.
Gao
Dayong
D
He
Li-Qun
LQ
Moey
Lip Kean
LK
Hua
Kegang
K
Liu
Zuo-Bin
ZB
D001792:Blood Platelets / Q000187:drug effects; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D008956:Models, Chemical; D019188:Organ Preservation Solutions / Q000737:chemistry* / Q000494:pharmacology; D019946:Propylene Glycol / Q000737:chemistry / Q000494:pharmacology; D012965:Sodium Chloride / Q000737:chemistry / Q000494:pharmacology; D013696:Temperature; D014867:Water / Q000737:chemistry / Q000494:pharmacology
D003451:Cryoprotective Agents; D019188:Organ Preservation Solutions; D014867:Water; D012965:Sodium Chloride; D019946:Propylene Glycol