The immunological properties of adult hippocampal progenitor cells.
Adult hippocampal progenitor cells (AHPCs) derived from mature rats were studied in mixed co-cultures and shown not to elicit a proliferative response from human peripheral blood mononuclear cells (PBMCs) or allogeneic spleen cells. FACS analysis revealed low class I and no detectable class II (Ia) MHC expression by these cells. RT-PCR showed that AHPCs express the anti-inflammatory cytokine TGF-beta1. AHPCs did not, however, significantly impede the proliferation of OKT3- or PHA-stimulated PBMCs. Taken together, these results indicate that AHPCs are non-immunogenic in vitro. This is consistent with their pattern of MHC expression and does not require an active immunosuppressive mechanism.
Klassen
Henry
H
Stem Cell Research, Children's Hospital of Orange County, 455 South Main Street, Orange, CA 92868, USA. hklassen@choc.org
Imfeld
Karen L
KL
Ray
Jasodhara
J
Young
Michael J
MJ
Gage
Fred H
FH
Berman
Monique A
MA
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D018920:Coculture Techniques; D017077:Culture Media, Conditioned; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D006624:Hippocampus / Q000166:cytology / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D008875:Middle Aged; D051381:Rats; D011916:Rats, Inbred F344; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000166:cytology; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000276:immunology*
D017077:Culture Media, Conditioned; D016207:Cytokines; D015395:Histocompatibility Antigens Class I