Peptide nucleic acid-metal complex conjugates: facile modulation of PNA-DNA duplex stability.
Conjugates of peptide nucleic acids (PNA) and metal binding ligands were prepared using solid-phase synthesis. Stability of duplexes of bis-picolylamine-PNA conjugates and DNA was found to be modulated by equimolar concentrations of bioavailable metal ions: Ni(2+), Zn(2+)>Cu(2+). Sequence specificity of PNA was not compromised in the presence of these metal ions.
Mokhir
Andriy
A
Anorganisch-Chemisches Institut, Universität Heidelberg, Im Neuenheimer Feld 270, Germany.
Stiebing
Roman
R
Kraemer
Roland
R
D020137:Base Pair Mismatch; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D019216:Metals, Heavy / Q000737:chemistry*; D009691:Nucleic Acid Denaturation; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry*; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D019216:Metals, Heavy; D009692:Nucleic Acid Heteroduplexes; D020135:Peptide Nucleic Acids; D004247:DNA