Semicarbazide-sensitive amine oxidases in pig dental pulp.
The behaviour of semicarbazide-sensitive amine oxidase (SSAO; E.C. 1.4.3.6) in dental pulp has been studied, with particular reference to the metabolism of 5-hydroxytryptamine (5-HT; serotonin). Kinetic studies using radioactively labelled substrates have confirmed benzylamine, 2-phenylethylamine (PEA) and 5-HT to be substrates for microsomal SSAO from porcine dental pulp. Kinetic substrate-competition studies indicated the presence of two forms of SSAO in dental pulp; one that oxidises benzylamine and PEA but not 5-HT and a second that oxidises 5-HT and PEA but not benzylamine. These two forms also differ in their thermostabilities at 60 and 70 degrees C, although this thermal inactivation is partly reversible.
O'Sullivan
Michael
M
Department of Restorative Dentistry and Periodontology, Dublin Dental School and Hospital, Lincoln Place, Dublin 2, Ireland. misullvn@dental.tcd.ie
MacDougall
Mary B
MB
Unzeta
Mercedes
M
Lizcano
Jose-Miguel
JM
Tipton
Keith F
KF
D006631:Amine Oxidase (Copper-Containing) / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D001596:Benzylamines / Q000378:metabolism; D003641:Deamination; D003782:Dental Pulp / Q000201:enzymology*; D004795:Enzyme Stability; D006358:Hot Temperature; D010084:Oxidation-Reduction; D012663:Semicarbazides / Q000494:pharmacology*; D012701:Serotonin / Q000378:metabolism; D013552:Swine
D001596:Benzylamines; D012663:Semicarbazides; D012701:Serotonin; C030796:benzylamine; D006631:Amine Oxidase (Copper-Containing)