[Preparation and characterization of monoclonal antibody against human telomeric repeat binding factor 1].
OBJECTIVE
To prepare a monoclonal antibody against human telomeric repeat binding factor 1 (TRF1) protein and explore its biological characteristics.


METHODS
BALB/c mice were immunized with GST-TRF1(33-277) fusion protein for the preparation of monoclonal antibody by hybridoma technique. The obtained antibody was used for clinical assay by Western-blot and immunohistochemical staining.


RESULTS
One strain of hybridoma was obtained. It was confirmed by Western-blot that the antibody specifically recognized the 60 kD TRF1 protein. Immunohistochemical staining of the antibody showed that TRF1 protein located in the cytoplasm of epithelial cells and bone marrow cells.


CONCLUSIONS
A TRF1 monoclonal antibody, with high specificity was developed. It is useful for detection of TRF1 protein in tissue specimens.
Huang
He
H
The First Affiliated Hospital, Medical College of Zhejiang University, Hangzhou 310003, China.
Shi
Jimin
J
Chen
Qiaofang
Q
Luo
Yi
Y
Ding
Wei
W
Lou
Jiyu
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015153:Blotting, Western; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D011993:Recombinant Fusion Proteins / Q000276:immunology; D035321:Telomeric Repeat Binding Protein 1 / Q000032:analysis / Q000235:genetics / Q000276:immunology*
D000911:Antibodies, Monoclonal; D011993:Recombinant Fusion Proteins; D035321:Telomeric Repeat Binding Protein 1