title: Antioxidative enzymes in human nasal mucosa after exposure to ozone. Possible role of GSTM1 deficiency.
abstract: OBJECTIVE
  Epithelial antioxidative enzymes (AOEs) are thought to be a first line of defense against reactive oxygen species as they are upregulated after exposure to ozone according to animal studies. We analysed the activities of the AOEs catalase (CAT), glutathione peroxidase (GPX), glutathione reductase (GR), superoxide dismutase (SOD) and glutathione-S-transferase (GST) in a tissue culture of human nasal mucosa and analysed the influence of GSTM1 polymorphism on AOE regulation.
  
  
  METHODS
  Tissue biopsies of 20 subjects were incubated for 24 h with and without 120 ppb ozone. Activities were assayed to determine what enzymatic changes had taken place, both overall and in regard to GSTM1 status.
  
  
  RESULTS
  Activities for GPX (p = 0.272) and SOD (p = 0.291) were found increased after ozone exposure. GSTM1-deficient patients showed a significantly enhanced upregulation of SOD activity (p = 0.011) compared to GSTM1 carriers.
  
  
  CONCLUSIONS
  Our findings suggest that GSTM1-deficiency has an impact on AOE-regulation after ozone exposure.
authors.affiliation: Allergy and Asthma Clinic, Charité, Virchow Klinikum, Humboldt University, Augustenbuger Platz 1, 13353 Berlin, Germany. ralf.otto-knapp@charite.de
authors.forename: R
authors.initials: R
authors.lastname: Otto-Knapp
authors.forename: K
authors.initials: K
authors.lastname: Jurgovsky
authors.forename: K
authors.initials: K
authors.lastname: Schierhorn
authors.forename: G
authors.initials: G
authors.lastname: Kunkel
mesh_terms: D005838:Genotype; D005982:Glutathione Transferase / Q000172:deficiency* / Q000235:genetics; D006579:Heterozygote; D006636:Histamine Release; D006801:Humans; D009297:Nasal Mucosa / Q000187:drug effects* / Q000201:enzymology* / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism*; D010126:Ozone / Q000494:pharmacology*; D011110:Polymorphism, Genetic
chemical_list: D005982:Glutathione Transferase; D010088:Oxidoreductases; D010126:Ozone