title: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for mtDNA typing in hokkaido brown bear (Ursus arctos yesoensis).
abstract: To develop an easy method of typing of mitochondrial DNA (mtDNA) in Hokkaido brown bears (Ursus arctos yesoensis), the PCR-RFLP technique was improved using four restriction enzymes: Mbo 1, Cfr 13 I, TspE 1, and Fok 1. This approach identified seven groups of mtDNA haplotypes, HB1/2/5-7, HB 3, HB4, HB8/9, HB10/11, HB12 and HB13 from 102 brown bears of northern, central and eastern Hokkaido.
authors.affiliation: Laboratory of Wildlife Biology, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Satoh
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Mano
authors.forename: Hifumi
authors.initials: H
authors.lastname: Tsuruga
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Masuda
authors.forename: Tamako
authors.initials: T
authors.lastname: Matsuhashi
authors.forename: Manabu
authors.initials: M
authors.lastname: Onuma
authors.forename: Masatsugu
authors.initials: M
authors.lastname: Suzuki
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Ohtaishi
mesh_terms: D000818:Animals; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics*; D006239:Haplotypes; D007564:Japan; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D001503:Ursidae / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial