title: Distribution and localization of caveolin-1 in sinusoidal cells in rat liver.
abstract: Caveolin, the principal structural protein in caveolae, is involved in signal transduction. The aim of the present study was to clarify the distribution and ultrastructural localization of caveolin-1 in hepatic sinusoidal endothelial cells (SECs) and hepatic stellate cell (HSCs) by confocal microscopy and the electron immunogold method. Liver tissue sections were prepared from male Wistar rats. SECs and HSCs were isolated from rat livers by collagenase infusion. For immunohistochemistry, liver sections were reacted with anticaveolin-1 antibody. The localization and distribution of caveolin-1 were identified by confocal immunofluorescence. The ultrastructural localization of caveolin-1 on SECs and HSCs was identified by electron microscopy using the immunogold method. Immunohistochemical studies using liver tissues localized caveolin-1 in sinusoidal lining cells, bile canaliculi, portal vein, and hepatic artery. By confocal microscopy, caveolin-1 was mainly demonstrated at the Golgi complex in SECs and HSCs. Under an electron microscope, immunogold particles indicating the presence of caveolin-1 were demonstrated on the plasma membrane of sinusoidal endothelial fenestrae (SEF) and vesicles in SECs. Under an electron microscope, immunogold particles indicating the presence of caveolin-1 were demonstrated on the plasma membrane of caveolae and vesicles in HSCs. We concluded that caveolin-1 is localized from SEFs to the Golgi complex in SECs and from caveolae to the Golgi complex in HSCs.
authors.affiliation: Laboratory of Pathology, Kitasato Institute Medical Center Hospital, 6-100 Arai, Kitamoto 364-8501, Japan. ogi-m@kitasato.or.jp
authors.forename: Mariko
authors.initials: M
authors.lastname: Ogi
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Yokomori
authors.forename: Masaya
authors.initials: M
authors.lastname: Oda
authors.forename: Kazunori
authors.initials: K
authors.lastname: Yoshimura
authors.forename: Masahiko
authors.initials: M
authors.lastname: Nomura
authors.forename: Susumu
authors.initials: S
authors.lastname: Ohshima
authors.forename: Masumi
authors.initials: M
authors.lastname: Akita
authors.forename: Kyoko
authors.initials: K
authors.lastname: Toda
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Ishii
mesh_terms: D000818:Animals; D021941:Caveolae / Q000378:metabolism / Q000648:ultrastructure; D051242:Caveolin 1; D022461:Caveolins / Q000378:metabolism*; D004727:Endothelium / Q000378:metabolism / Q000648:ultrastructure; D006056:Golgi Apparatus / Q000378:metabolism / Q000648:ultrastructure; D007150:Immunohistochemistry; D008099:Liver / Q000378:metabolism* / Q000648:ultrastructure*; D008297:Male; D018613:Microscopy, Confocal; D016253:Microscopy, Immunoelectron; D051381:Rats; D017208:Rats, Wistar
chemical_list: C495537:Cav1 protein, rat; D051242:Caveolin 1; D022461:Caveolins