Inhibition of larval development of the marine copepod Acartia tonsa by four synthetic musk substances.
A nitro musk (musk ketone) and three polycyclic musks (Tonalide, Galaxolide and Celestolide) were tested for acute and subchronic effects on a marine crustacean, the calanoid copepod Acartia tonsa. Sublethal effects on A. tonsa larvae were investigated with a rapid and cost effective bioassay, which is based on the easily detectable morphological change from the last nauplius to the first copepodite stage during copepod larval development. The inhibition of larval development after 5 days exposure was a very sensitive endpoint, with 5-d-EC(50)-values as low as 0.026 mg/l (Tonalide), 0.059 mg/l (Galaxolide), 0.066 mg/l (musk ketone) and 0.160 mg/l (Celestolide), respectively. These values were generally more than one order of magnitude below the 48-h-LC(50)-values found for adults, which were 0.47 mg/l (Galaxolide), 0.71 mg/l (Celestolide), 1.32 mg/l (musk ketone) and 2.5 mg/l (Tonalide). Since the synthetic musks strongly inhibited larval development in A. tonsa at low nominal concentrations, they should be considered as very toxic. The larval development test with A. tonsa is able to provide important aquatic toxicity data for the evaluation of synthetic musks, for which there is little published ecotoxicological information available regarding Crustacea. It is suggested that subchronic and chronic copepod toxicity tests should be used more frequently for risk assessment of environmental pollutants.
Wollenberger
Leah
L
Environment and Resources DTU, Technical University of Denmark, Building 115, DK-2800 Kgs, Lyngby, Denmark. lew@er.dtu.dk
Breitholtz
Magnus
M
Ole Kusk
Kresten
K
Bengtsson
Bengt Erik
BE
D000818:Animals; D000970:Antineoplastic Agents / Q000009:adverse effects*; D001578:Benzopyrans / Q000009:adverse effects*; D001681:Biological Assay; D033342:Copepoda / Q000254:growth & development*; D004305:Dose-Response Relationship, Drug; D007189:Indans / Q000009:adverse effects*; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D007928:Lethal Dose 50; D010476:Perfume / Q000009:adverse effects*; D012680:Sensitivity and Specificity; D013764:Tetrahydronaphthalenes / Q000009:adverse effects*; D014874:Water Pollutants, Chemical / Q000009:adverse effects*; D014992:Xylenes / Q000009:adverse effects*
D000970:Antineoplastic Agents; D001578:Benzopyrans; D007189:Indans; D010476:Perfume; D013764:Tetrahydronaphthalenes; D014874:Water Pollutants, Chemical; D014992:Xylenes; C033118:acetyl tert-butyl dimethylindan; C033119:galaxolide; C033116:acetyl methyl tetramethyl tetralin; C031448:musk ketone