Purification and characterization of class-I and class-II fructose-1,6-bisphosphate aldolases from the cyanobacterium Synechocystis sp. PCC 6803.
The whole genome sequence database for Synechocystis sp. PCC 6803 has revealed the presence of genes encoding class-I (CI) and class-II (CII) fructose-1,6-bisphosphate aldolases (FBAs) in this organism. Two types of FBA from Synechocystis sp. PCC 6803 were separated by chromatography on phenyl-Sepharose. The activity of the enzyme in the major peak was inhibited by the presence of 25 mM EDTA; however, the activity in the minor peak was not. Therefore, the FBA in the former fractions was designated as CII-FBA, and in the latter designated as CI-FBA. CI-FBA was functionally redundant in Synechocystis sp. PCC 6803, while no disruptant for the gene encoding CII-FBA was obtained under photoautotrophic conditions. The kinetic parameters of CI- and CII-FBAs purified from Synechocystis sp. PCC 6803 in the cleavage reaction of FBP were generally similar, except in their reactivity for SBP. The SBP/FBP activity ratio of the CII-FBA was two times higher than that of the CI-FBA.
Nakahara
Ken
K
Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara, 630-0101 Japan.
Yamamoto
Hiroshi
H
Miyake
Chikahiro
C
Yokota
Akiho
A
D000458:Cyanobacteria / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D004492:Edetic Acid / Q000494:pharmacology; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D005635:Fructosediphosphates / Q000378:metabolism*; D007700:Kinetics; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D013403:Sugar Phosphates / Q000378:metabolism
D005635:Fructosediphosphates; D013403:Sugar Phosphates; C022079:sedoheptulose 1,7-bisphosphate; D004492:Edetic Acid; D005634:Fructose-Bisphosphate Aldolase