title: Mononuclear cell antigen expression after contact with one-day disposable contact lens materials.
abstract: OBJECTIVE
  To analyse the effects of 1-day disposable contact lens (CL) material on mononuclear cell activation antigens and adhesion molecules, in an in vitro model set up in order to simulate the first 2 h of wear.
  
  
  METHODS
  Etafilcon A and Hilafilcon A materials were analysed. Mononuclear cells, collected from peripheral blood samples of normal human donors, were placed in contact with the materials, both new and coated with artificial tear solution. As a control, Thermanox coverslips and a group with no material were included. Immunofluorescence reactions were carried out on cells, to study the distribution of activation (HLA-DR, CD25, CD69) and adhesion (CD11a, CD18, CD54) molecules; the flow cytometry technique was used to analyse cells.
  
  
  RESULTS
  Statistically significant results were demonstrated for the CD69 and all the 3 adhesion molecules. Artificial tear coating enhanced the mononuclear cell response.
  
  
  CONCLUSIONS
  The daily mononuclear cell activation can lead, with time, to production of inflammatory mediators that may be responsible for discomfort or, at worst, intolerance and definitely to CL wear remittance.
authors.affiliation: Centre of Biotechnological and Clinical Research in Ophthalmology, University of Bologna, Bologna, Italy. versura@alma.unibo.it
authors.forename: Piera
authors.initials: P
authors.lastname: Versura
authors.forename: Sandra
authors.initials: S
authors.lastname: Iannelli
authors.forename: Sergio
authors.initials: S
authors.lastname: Stefoni
authors.forename: Roberto
authors.initials: R
authors.lastname: Caramazza
mesh_terms: D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism*; D001672:Biocompatible Materials / Q000009:adverse effects*; D001769:Blood / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D003261:Contact Lenses / Q000009:adverse effects* / Q000145:classification; D003263:Contact Lenses, Hydrophilic / Q000009:adverse effects; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007150:Immunohistochemistry; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism*; D013997:Time Factors
chemical_list: D015703:Antigens, CD; D001672:Biocompatible Materials; D015815:Cell Adhesion Molecules