Lytic and contracture-lytic prenecrotic damage to cardiomyocytes: photochemical fluorochrome staining and fluorescent microscopy of the myocardium.
Acute prenecrotic damage to cardiomyocytes of lytic (myocytolysis, cytolysis) and contracture-lytic (primary lumpy degradation of myofibrils) types during ischemic and metabolic alteration of the myocardium are detected at the photooptic level by means of photochemical fluorochrome staining and examination under fluorescent light. Comparison of the fluorescent and polarization microscopic pictures showed that changes in cardiomyocytes are determined by local mass redistribution in the sarcomere compartments and transformations of birefraction of myofibrillar system components during necrobiosis. These changes are determined by lysis and coagulation processes in protein structures of sarcomeres.
Zimmermann
V G
VG
Department of General Pathology and Pathomorphology, Institute of Regional Pathology and Pathomorphology, Siberian Division of Russian Academy of Medical Sciences, Novosibirsk, Russia. zimmermann@bionet.nsc.ru
Nepomnyashchikh
L M
LM
D000818:Animals; D016880:Anisotropy; D009202:Cardiomyopathies / Q000473:pathology*; D004285:Dogs; D005456:Fluorescent Dyes; D006801:Humans; D051379:Mice; D008856:Microscopy, Fluorescence; D008859:Microscopy, Polarization; D009200:Myocardial Contraction; D009203:Myocardial Infarction / Q000473:pathology; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000473:pathology*; D009210:Myofibrils / Q000473:pathology; D009336:Necrosis; D051381:Rats; D012518:Sarcomeres / Q000378:metabolism; D012519:Sarcoplasmic Reticulum / Q000378:metabolism; D013194:Staining and Labeling
D005456:Fluorescent Dyes