Comparative antioxidant activity of individual herbal components used in Ayurvedic medicine.
Four aqueous extracts from different parts of medicinal plants used in Ayurveda (an ancient Indian Medicine) viz., Momardica charantia Linn (AP1), Glycyrrhiza glabra (AP2), Acacia catechu (AP3), and Terminalia chebula (AP4) were examined for their potential as antioxidants. The antioxidant activity of these extracts was tested by studying the inhibition of radiation induced lipid peroxidation in rat liver microsomes at different doses in the range of 100-600 Gy as estimated by thiobarbituric acid reactive substances (TBARS). Of all these extracts, AP4 showed maximum inhibition in the TBARS formation and hence is considered the best antioxidant among these four extracts. The extracts were found to restore antioxidant enzyme superoxide dismutase (SOD) from the radiation induced damage. The antioxidant capacities were also evaluated in terms of ascorbate equivalents by different methods such as cyclic voltammetry, decay of ABTS(.-) radical by pulse radiolysis and decrease in the absorbance of DPPH radicals. The results were found to be in agreement with the lipid peroxidation data and AP4 showed maximum value of ascorbate equivalents. Therefore AP4, with high antioxidant activity, is considered as the best among these four extracts.
Naik
G H
GH
Radiation Chemistry and Chemical Dynamics Division, Bhabha Atomic Research Centre, Trombay, Mumbai-400085, India.
Priyadarsini
K I
KI
Satav
J G
JG
Banavalikar
M M
MM
Sohoni
D P
DP
Biyani
M K
MK
Mohan
H
H
D000818:Animals; D000975:Antioxidants / Q000032:analysis* / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000737:chemistry; D001713:Biphenyl Compounds; D004305:Dose-Response Relationship, Drug; D004563:Electrochemistry / Q000379:methods; D005609:Free Radicals / Q000737:chemistry / Q000378:metabolism; D005720:Gamma Rays; D020128:Inhibitory Concentration 50; D007700:Kinetics; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects; D008515:Medicine, Ayurvedic; D008862:Microsomes, Liver / Q000187:drug effects / Q000528:radiation effects; D008930:Mitochondria, Liver / Q000187:drug effects / Q000528:radiation effects; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry*; D011675:Pulse Radiolysis / Q000379:methods; D051381:Rats; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis / Q000378:metabolism
D000975:Antioxidants; D001713:Biphenyl Compounds; D005609:Free Radicals; D010851:Picrates; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; C004931:1,1-diphenyl-2-picrylhydrazyl; D013482:Superoxide Dismutase; D001205:Ascorbic Acid