Importance of Protocol Immunization in Epitope Selectivity of Monoclonal Antibodies Interacting with Binding Subunit of Viscumin.
The application of two immunization protocols and two screening systems has allowed to produce five hybridomas mlb5, mlb6, mlb7, mlb9 and Mbch1, secreting mAbs against different sites of viscumin B-subunit. On the base of mlb9 and Mbch1 hybridomas, the test-system has been developed, able to detect up to 5 ng/ml of viscumin and up to 1 ng/ml of its B-chain. Produced hybridomas and monoclonal antibodies will be used for the studies of intracellular transport of plant toxins. Monoclonal antibody mlb7 will be used for the studies of viscumin interactions with immune system of mammals.
Moisenovich
Mikhail M.
MM
M.V. Lomonosov Moscow State University, Moscow, Russia.
Egorova
Svetlana G.
SG
Chelnokova
Olga V.
OV
Demina
Irina A.
IA
Polosukhina
Elena R.
ER
Kozlovskaya
Natalia V.
NV
Popova
Ekaterina N.
EN
Fattakhova
Gulnar V.
GV
Solopova
Olga N.
ON
Agapov
Igor I.
II