title: Adhesion and colonization of enterohemorrhagic Escherichia coli O157:H7 in cecum of mice.
abstract: Infectious diseases due to enterohemorrhagic Escherichia coli (EHEC) are characterized by diarrhea, hemorrhagic colitis and hemolytic uremic syndrome. The adherence of EHEC on intestinal epithelial cells is a first step for developing these diseases. In the present study, we examined whether EHEC O157:H7 adhere to intestinal epithelial cells of mice and cause F-actin accumulation in the epithelial cells following the intragastric inoculation of the pathogen. Fecal shedding of the EHEC O157:H7 strain was observed in ICR mice up to 3 weeks. Fecal shedding periods of the type III secretion system-related gene (espA and sepL) deletion mutants were clearly shorter than that of the wild-type EHEC O157:H7 strain. The EHEC O157:H7 colonies were found on the epithelial surfaces of the ceca in association with F-actin accumulation beneath the attached bacteria.
authors.affiliation: Laboratory of Microbiology, Department of Public Health Pharmacy, Gifu Pharmaceutical University, Gifu, Gifu 502-8585, Japan.
authors.forename: Keiji
authors.initials: K
authors.lastname: Nagano
authors.forename: Kazuki
authors.initials: K
authors.lastname: Taguchi
authors.forename: Takumi
authors.initials: T
authors.lastname: Hara
authors.forename: Shin-ichiro
authors.initials: S
authors.lastname: Yokoyama
authors.forename: Kenji
authors.initials: K
authors.lastname: Kawada
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mori
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001422:Bacterial Adhesion; D002432:Cecum / Q000382:microbiology*; D004847:Epithelial Cells / Q000382:microbiology; D004927:Escherichia coli Infections / Q000382:microbiology; D019453:Escherichia coli O157 / Q000472:pathogenicity* / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000235:genetics; D005243:Feces / Q000382:microbiology; D005260:Female; D017353:Gene Deletion; D006367:HeLa Cells; D006463:Hemolytic-Uremic Syndrome / Q000382:microbiology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008813:Mice, Inbred ICR; D018613:Microscopy, Confocal
chemical_list: D000199:Actins; D029968:Escherichia coli Proteins; C102051:EspA protein, E coli; C416081:SepL protein, E coli