DHEA administration increases brown fat uncoupling protein 1 levels in obese OLETF rats.
We found that UCP-1 and UCP-3 mRNA expression levels and the UCP-1 protein content in brown adipose tissue (BAT) were reduced in prediabetic OLETF rats than the lean LETO rats. Administration of dehydroepiandrosterone (DHEA) for 17 days induced remarkable weight loss, which was in part attributed to an enhanced utilization of ingested energy. DHEA administration significantly increased the levels of BAT UCP-1 and UCP-3 mRNA expression. Among the upstream signals for UCP-1 regulation, expression levels of the beta 3 adrenergic receptor (beta(3)AR) and PPAR gamma coactivator-1 (PGC-1) were significantly decreased in the OLETF rats and increased by DHEA administration. The decreased expression levels of UCP-1 and its upstream regulators, beta(3)AR and PGC-1, in BAT may contribute to inefficient energy utilization and obesity in OLETF rats, which was corrected by DHEA treatment.
Ryu
Je-Won
JW
Asan Institute for Life Sciences, University of Ulsan College of Medicine, Poongnap-dong, Songpa-ku, Seoul 138-736, Republic of Korea.
Kim
Min-Seon
MS
Kim
Chan-Hee
CH
Song
Kee-Ho
KH
Park
Joong-Yeol
JY
Lee
Jae-Dam
JD
Kim
Jae-Bum
JB
Lee
Ki-Up
KU
D002001:Adipose Tissue, Brown / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002352:Carrier Proteins / Q000187:drug effects / Q000378:metabolism*; D003687:Dehydroepiandrosterone / Q000494:pharmacology*; D002149:Energy Intake; D004734:Energy Metabolism / Q000187:drug effects; D007473:Ion Channels; D008565:Membrane Proteins / Q000187:drug effects / Q000378:metabolism*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D024101:Mitochondrial Proteins; D051381:Rats; D020334:Rats, Inbred OLETF; D000071256:Uncoupling Protein 1
D002352:Carrier Proteins; D007473:Ion Channels; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; C000604373:Ucp1 protein, rat; D000071256:Uncoupling Protein 1; D003687:Dehydroepiandrosterone