[An artificial protein, possessing biological activity of HL-60 human promyelocytic leukemia differentiation factor].
The ABB-df artificial protein was prepared by inserting the TGENHR biologically active peptide corresponding to the 41-46 sequence of the differentiation factor for the HL-60 cell line of the human promyelocyte leukemia into the N-terminus of the polypeptide chain of albebetin, an artificial protein with the preset structure. The ABB-df protein was found to induce the differentiation of HL-60 cells and to inhibit their proliferation; its efficiency was almost the same as that of the starting peptide. According to CD spectroscopy, the inclusion of the peptide fragment into albebetin exerts virtually no effect on the regular secondary structure of albebetin.
Chertkova
R V
RV
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, GSP Moscow, 117997 Russia. rita@nmr.ru
Kostanian
I A
IA
Astapova
M V
MV
Surina
E A
EA
Dolgikh
D A
DA
Kirpichnikov
M P
MP
D000595:Amino Acid Sequence; D001483:Base Sequence; D002454:Cell Differentiation; D002942:Circular Dichroism; D017931:DNA Primers; D018922:HL-60 Cells; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000378:metabolism* / Q000473:pathology; D008969:Molecular Sequence Data; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*
D017931:DNA Primers; D011506:Proteins