Genomic amplification and oncogenic properties of the KCNK9 potassium channel gene.
Representational difference analysis (RDA) of human breast cancer was used to discover a novel amplicon located at chromosomal region 8q24.3. We examined a series of breast cancer samples harboring amplification of this region and determined that KCNK9 is the sole overexpressed gene within the amplification epicenter. KCNK9 encodes a potassium channel that is amplified from 3-fold to 10-fold in 10% of breast tumors and overexpressed from 5-fold to over 100-fold in 44% of breast tumors. Overexpression of KCNK9 in cell lines promotes tumor formation and confers resistance to both hypoxia and serum deprivation, suggesting that its amplification and overexpression plays a physiologically important role in human breast cancer.
Mu
David
D
Tularik Inc, Genomics Division, 266 E Pulaski Road, Greenlawn, NY 11740, USA. dmu@tularik.com
Chen
Liyun
L
Zhang
Xiping
X
See
Lei-Hoon
LH
Koch
Christina M
CM
Yen
Clifford
C
Tong
James Jiayuan
JJ
Spiegel
Lori
L
Nguyen
Ken C Q
KC
Servoss
Allyson
A
Peng
Yue
Y
Pei
Lin
L
Marks
Jeffrey R
JR
Lowe
Scott
S
Hoey
Timothy
T
Jan
Lily Yeh
LY
McCombie
W Richard
WR
Wigler
Michael H
MH
Powers
Scott
S
D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics; D002460:Cell Line; D017690:Cell Transplantation; D002898:Chromosomes, Human, Pair 8; D015342:DNA Probes; D005260:Female; D005784:Gene Amplification; D015972:Gene Expression Regulation, Neoplastic; D015894:Genome, Human; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009857:Oncogenes; D020161:Physical Chromosome Mapping; D015221:Potassium Channels / Q000235:genetics* / Q000378:metabolism*; D016324:Sequence Tagged Sites; D014183:Transplantation, Heterologous
D015342:DNA Probes; D015221:Potassium Channels