title: Activation of the aryl hydrocarbon receptor by some vegetable constituents determined using in vitro reporter gene assay.
abstract: The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that mediates the biological action of many aromatic environmental pollutants. In this study, we investigated the activation of the AhR by some vegetable constituents using the AhR-based bioassay for dioxins, i.e., the chemical activated luciferase gene expression (CALUX) assay. Ninety-five vegetable constituents, including flavonoids, tannins, saponins, and terpenes, were tested in vitro. Among them, isoflavones such as daidzein, resveratrol having a stilbene structure, and some flavonoids such as naringenin, hesperetin, and baicalein showed AhR activation.
authors.affiliation: Division of Foods, National Institute of Health Sciences, Tokyo, Japan. amakura@nihs.go.jp
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Amakura
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Tsutsumi
authors.forename: Masafumi
authors.initials: M
authors.lastname: Nakamura
authors.forename: Hiroko
authors.initials: H
authors.lastname: Kitagawa
authors.forename: Junko
authors.initials: J
authors.lastname: Fujino
authors.forename: Kumiko
authors.initials: K
authors.lastname: Sasaki
authors.forename: Masatake
authors.initials: M
authors.lastname: Toyoda
authors.forename: Takashi
authors.initials: T
authors.lastname: Yoshida
authors.forename: Tamio
authors.initials: T
authors.lastname: Maitani
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000502:physiology; D017930:Genes, Reporter / Q000502:physiology*; D007529:Isoflavones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D018336:Receptors, Aryl Hydrocarbon / Q000819:agonists / Q000235:genetics / Q000378:metabolism*; D014675:Vegetables
chemical_list: D007529:Isoflavones; D018336:Receptors, Aryl Hydrocarbon; D008156:Luciferases