[Molecular cloning of TSARG3 gene related to apoptosis in human spermatogenic cells].
OBJECTIVE
To clone a novel gene which is related to human testis spermatogenesis apoptosis.


METHODS
To rapidly attain human novel gene full-length cDNA sequence from a human testis cDNA library,the gene-specific primers and the vector-specific primers were designed for nested polymerase chain reaction. Sequencing was performed and the result was analysed.


RESULTS
The present authors discovered the TSARG3 gene(GenBank accession number AF419291) from a human testis cDNA library, using a cDNA fragment (GenBank accession number BE644537) as an electronic probe, which was significantly changed in cryptorchidism and represented a novel gene. Furthermore, a mouse homologue of this gene was identified (GenBank accession number AF419292) by using the same method.


CONCLUSIONS
A novel gene named TSARG3 was cloned. It is considered that the function of the new gene is related to human testis spermatogenesis apoptosis.
Liu
Gang
G
Human Reproductive Engineering Institute, Xiangya Medical School, Central South University, Changsha, Hunan, 410078 P. R. China. lgxdiredtor@sina.com
Lu
Guangxiu
G
Fu
Junjiang
J
Liu
Shangfeng
S
Xing
Xiaowei
X
Li
Luyun
L
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D051017:Apoptosis Regulatory Proteins; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006360:Heat-Shock Proteins; D006801:Humans; D008297:Male; D051379:Mice; D018832:Molecular Chaperones; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013090:Spermatocytes / Q000166:cytology / Q000378:metabolism*
D051017:Apoptosis Regulatory Proteins; D018076:DNA, Complementary; C477075:DNAJB13 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D011506:Proteins