title: [Synergistic effect of toremifene and cisplatin on human lung cancer cell line A549].
abstract: OBJECTIVE
  To study the toxic effect of toremifene (TOR) and its synergistic effect with cisplatin (DDP) on human lung adenocarcinoma cell line A549.
  
  
  METHODS
  The cytotoxic effects of these agents on human lung cancer cell line A549 were monitored by a tetrazolium-based colorimetric assay (MTT assay). The cell cycle and DNA content were detected by flow cytometer technic. p21 expression level was monitored by Western blot.
  
  
  RESULTS
  Toremifene inhibited the growth of A549 cell, with > or = 5 micromol/L significantly enhancing the chemosensitivity of cisplatin. TOR enhanced the antitumor activity of DDP at S, G(2) and M phases of cells. And p21 expression was increased after TOR and DDP had been given.
  
  
  CONCLUSIONS
  Toremifene (> or = 5 micromol/L) combined with cisplatin shows significant synergistic anti-tumor effect on A549 cells.
authors.affiliation: Department of Respiratory Medicine, Chang Hai Hospital, Second Military Medical University, Shanghai 200433, China.
authors.forename: Xueyan
authors.initials: X
authors.lastname: Zhang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.forename: Yiping
authors.initials: Y
authors.lastname: Han
authors.forename: Zhongling
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm; D004354:Drug Screening Assays, Antitumor; D004357:Drug Synergism; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D017312:Toremifene / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D017312:Toremifene; D002945:Cisplatin