Anti-C5a monoclonal antibodies and pulmonary polymorphonuclear leukocyte infiltration--endothelial dysfunction by venous gas embolism.
Venous gas embolism (VGE) impairs endothelial function although there is no apparent mechanical damage to the endothelial layer. We investigated whether a monoclonal antibody against the complement anaphylatoxine C5a would affect endothelial dysfunction and pulmonary polymorphonuclear leukocyte infiltration caused by low-grade VGE. Six rabbits were pre-treated with the anti-C5a monoclonal antibody whereas a sham monoclonal antibody was administrated to six other animals 30 min before VGE. Six untreated rabbits subjected to an identical protocol except antibody treatment were used for control. The monoclonal anti-C5a antibody reduced PMN infiltration compared to the control group ( P<0.03). There were no major signs of apoptosis in endothelial cells inside the pulmonary artery in any of the examined animals. There was reduced PMN infiltration and improved endothelium-dependent relaxation in the sham-antibody group, these effects were however not significant. In conclusion, anti-C5a protects the endothelium against injury caused by small amounts of gas bubbles.
Nossum
Vibeke
V
Department of Circulation and Imaging, Norwegian University of Science and Technology, N-7489 Trondheim, Norway. vibeke.nossum@medisin.ntnu.no
Hjelde
Astrid
A
Bergh
Kåre
K
Ustad
Anne-Lise
AL
Brubakk
Alf O
AO
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage* / Q000276:immunology; D017209:Apoptosis / Q000187:drug effects; D003167:Complement Activation / Q000187:drug effects / Q000276:immunology; D015936:Complement C5a / Q000032:analysis / Q000276:immunology*; D004618:Embolism, Air / Q000276:immunology* / Q000473:pathology; D004730:Endothelium, Vascular / Q000187:drug effects / Q000276:immunology / Q000293:injuries / Q000503:physiopathology*; D005260:Female; D007275:Injections, Intravenous; D008168:Lung / Q000187:drug effects / Q000276:immunology* / Q000473:pathology; D008297:Male; D020556:Neutrophil Infiltration / Q000187:drug effects / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology* / Q000473:pathology; D009599:Nitroprusside / Q000494:pharmacology; D011651:Pulmonary Artery / Q000187:drug effects; D011817:Rabbits; D014665:Vasodilator Agents / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D014665:Vasodilator Agents; D009599:Nitroprusside; D015936:Complement C5a; D000109:Acetylcholine