title: A naturally occurring mutation in an ATP-binding domain of the recombination repair gene XRCC3 ablates its function without causing cancer susceptibility.
abstract: Inherited mutations of the BRCA1 and BRCA2 genes, whose protein products are necessary for the homology-directed DNA repair pathway, confer a dominant susceptibility to cancer. We have investigated whether mutations of genes encoding other components of the same DNA repair pathway can also affect cancer susceptibility. We have identified three novel non-synonymous substitutions in one such gene, encoding the RAD51-related protein XRCC3. One of these variants, D213N, occurs in a highly conserved ATP-binding domain and completely abrogates the ability of the transfected gene to correct the phenotype of XRCC3 deficient cells. The D213N variant was found in the heterozygous state in DNA from 3/1577 healthy individuals. However, we did not detect this variant at all amongst 187 breast cancer families and 1300 unrelated patients with common cancers. Thus we have no evidence that D213N increases the risk of cancer. We propose that not all components of the homologous recombination repair complex can act as cancer susceptibility genes.
authors.affiliation: Institute for Cancer Studies, Division of Genomic Medicine, University of Sheffield Medical School, Beech Hill Road, Sheffield, S10 2RX, UK.
authors.forename: Saeed
authors.initials: S
authors.lastname: Rafii
authors.forename: Annika
authors.initials: A
authors.lastname: Lindblom
authors.forename: Malcolm
authors.initials: M
authors.lastname: Reed
authors.forename: Mark
authors.initials: M
authors.lastname: Meuth
authors.forename: Angela
authors.initials: A
authors.lastname: Cox
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000595:Amino Acid Sequence; D017124:Conserved Sequence; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D009369:Neoplasms / Q000235:genetics*; D011995:Recombination, Genetic; D016415:Sequence Alignment; D014162:Transfection
chemical_list: D004268:DNA-Binding Proteins; C110901:X-ray repair cross complementing protein 3; D000255:Adenosine Triphosphate