title: Escherichia coli response to exogenous pyrophosphate and analogs.
abstract: The addition of exogenous pyrophosphate increases the growth yield and cAMP synthesis in stationary phase when Escherichia coli is grown in minimal medium. Pyrophosphate increases the yield by altering the enterobactin uptake system. We studied the physiological effects and examined how the E. coli transcriptome was modified when two structural analogs of pyrophosphate were added to the growth medium. Methylenediphosphonic acid or a high concentration of iron had the same positive effects as pyrophosphate on growth yield, cAMP synthesis and the repression of Fur-regulated genes. In contrast, imidodiphosphate did not affect these cellular processes significantly. The transcriptome modifications generated by pyrophosphate or methylenediphosphonic acid were more similar than those generated by imidodiphosphate or excess iron. The transcriptome data also indicated that processes other than iron uptake might be involved in the cellular response to exogenous pyrophosphate or methylenediphosphonic acid.
authors.affiliation: Département de Biochimie et Génétique moléculaire, Institut Pasteur, Paris, France. fbiville@pasteur.fr
authors.forename: Francis
authors.initials: F
authors.lastname: Biville
authors.forename: Taku
authors.initials: T
authors.lastname: Oshima
authors.forename: Hirotada
authors.initials: H
authors.lastname: Mori
authors.forename: Yuya
authors.initials: Y
authors.lastname: Kawagoe
authors.forename: Odile
authors.initials: O
authors.lastname: Bouvet
authors.forename: Marie-Noëlle
authors.initials: MN
authors.lastname: Rager
authors.forename: Marina
authors.initials: M
authors.lastname: Perrotte-Piquemal
authors.forename: Antoine
authors.initials: A
authors.lastname: Danchin
mesh_terms: D003470:Culture Media; D000242:Cyclic AMP / Q000096:biosynthesis*; D011756:Diphosphates / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D004164:Diphosphonates / Q000378:metabolism / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D007501:Iron / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D020543:Proteome; D014158:Transcription, Genetic
chemical_list: D003470:Culture Media; D011756:Diphosphates; D004164:Diphosphonates; D029968:Escherichia coli Proteins; D020543:Proteome; C016514:imidodiphosphonic acid; C027474:methylene diphosphonate; D000242:Cyclic AMP; D007501:Iron