title: The cytokine markers in Staphylococcus aureus mastitis of bovine mammary gland.
abstract: TaqMan real time PCR was used to study the transcriptional activity of the bovine IL-2, IL-6, IL-12p40, IFN-gamma, TNF-alpha and granulocyte-monocyte colony stimulating factor of whole milk cells in bovine mammary gland experimentally infected with Staphylococcus aureus. Cytokine transcriptional activity was monitored at 7, 24 and 32 h Post-infection (Pi). IL-12 and TNF-alpha levels were significantly elevated at 24 h Pi followed by sharp decrease at 32 h pi. IL-2 level was decreased at 32 h pi. IL-12 and IFN-gamma showed a significant interaction at 24 h pi. The significant elevations of the IL-12 and TNF-alpha transcriptional level most likely indicate their important role in regulation of the immune responses of bovine mammary gland in S. aureus infection. Depression of IL-2 could reflect the suppressive nature of the S. aureus mastitis.
authors.affiliation: Department of Microbiology and Parasitology, College of Veterinary Medicine and Animal Resources, King Faisal University, PO Box 35252, Al-AHSAA, 31982 Saudi Arabia. alluwaimi@kfu.edu.sa
authors.forename: A M
authors.initials: AM
authors.lastname: Alluwaimi
authors.forename: C M
authors.initials: CM
authors.lastname: Leutenegger
authors.forename: T B
authors.initials: TB
authors.lastname: Farver
authors.forename: P V
authors.initials: PV
authors.lastname: Rossitto
authors.forename: W L
authors.initials: WL
authors.lastname: Smith
authors.forename: J S
authors.initials: JS
authors.lastname: Cullor
mesh_terms: D000818:Animals; D002417:Cattle; D016207:Cytokines / Q000235:genetics / Q000276:immunology*; D017931:DNA Primers; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D007371:Interferon-gamma; D018664:Interleukin-12; D053762:Interleukin-12 Subunit p40; D007376:Interleukin-2; D015850:Interleukin-6; D008414:Mastitis, Bovine / Q000276:immunology*; D008892:Milk / Q000166:cytology / Q000276:immunology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D021122:Protein Subunits; D012333:RNA, Messenger / Q000032:analysis; D013203:Staphylococcal Infections / Q000276:immunology*; D013211:Staphylococcus aureus; D014409:Tumor Necrosis Factor-alpha
chemical_list: D016207:Cytokines; D017931:DNA Primers; D053762:Interleukin-12 Subunit p40; D007376:Interleukin-2; D015850:Interleukin-6; D021122:Protein Subunits; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D018664:Interleukin-12; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor