title: Translocation of annexin I from cellular membrane to the nuclear membrane in human esophageal squamous cell carcinoma.
abstract: OBJECTIVE
  To investigate the alteration of the annexin I subcellular localization in esophageal squamous cell carcinoma (ESCC) and the correlation between the translocation and the tumorigenesis of ESCC.
  
  
  METHODS
  The protein localization of annexin I was detected in both human ESCC tissues and cell line via the indirect immunofluorescence strategy.
  
  
  RESULTS
  In the normal esophageal epithelia the annexin I was mainly located on the plasma membrane and formed a consecutive typical trammels net. Annexin I protein also expressed dispersively in cytoplasm and the nuclei without specific localization on the nuclear membrane. In esophageal cancer annexin I decreased very sharply with scattered disappearance on the cellular membrane, however it translocated and highly expressed on the nuclear membrane, which was never found in normal esophageal epithelia. In cultured esophageal cancer cell line annexin I protein was also focused on the nuclear membrane, which was consistent with the result from esophageal cancer tissues.
  
  
  CONCLUSIONS
  This observation suggests that the translocation of annexin I protein in ESCC may correlate with the tumorigenesis of the esophageal cancer.
authors.affiliation: National Lab. of Molecular Oncology, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing P. O. Box 2258, Beijing 100021, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.forename: Hui-Xin
authors.initials: HX
authors.lastname: Wang
authors.forename: Ning
authors.initials: N
authors.lastname: Lu
authors.forename: You-Sheng
authors.initials: YS
authors.lastname: Mao
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Hai-Rong
authors.initials: HR
authors.lastname: Zhang
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Wu
authors.forename: Xiao-Hang
authors.initials: XH
authors.lastname: Zhao
mesh_terms: D017305:Annexin A1 / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D002462:Cell Membrane / Q000378:metabolism; D004938:Esophageal Neoplasms / Q000378:metabolism* / Q000473:pathology; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007150:Immunohistochemistry; D009685:Nuclear Envelope / Q000378:metabolism*; D021381:Protein Transport
chemical_list: D017305:Annexin A1