title: Bioartificial pancreas transplantation at prevascularized intermuscular space: effect of angiogenesis induction on islet survival.
abstract: BACKGROUND
  Bioartificial pancreas (BAP) transplantation offers a potential treatment of diabetes mellitus. The optimal site for BAP transplantation has not yet been established.
  
  
  OBJECTIVE
  To monitor the effect of induction of neovascularization at the intermuscular space on islet survival after allogenic transplantation of BAP.
  
  
  METHODS
  Angiogenesis was induced at the intermuscular space of diabetic Lewis rats by implanting a polyethylene terephthalate (PET) mesh bag, which enclosed a collagen sponge and biodegradable gelatin microspheres containing basic fibroblast growth factor. After confirmation of angiogenesis, BAP was prepared by mixing of 5% agarose with approximately 2,800 isolated rat (Sprague-Dawley) islets and transplanted into the prevascularized PET mesh bag.
  
  
  RESULTS
  Neovascularization was observed in and around the PET mesh bag within 10 days after implantation as confirmed by macroscopic and microscopic examinations. In the presence of a collagen sponge, new blood vessels penetrated into the PET mesh bag and formed a vascular bed. After transplantation, normoglycemia was achieved in the rats within 3 days and maintained for >35 days. The rats gradually gained body weight, and the results of intravenous glucose tolerance test showed normal patterns of blood glucose clearance 1 month after transplantation.
  
  
  CONCLUSIONS
  It can be concluded that the prevascularized PET mesh bag enabled transplanted BAP to survive and maintain function, thus indicating a potential site for BAP transplantation.
authors.affiliation: Department of Organ Reconstruction, Field of Clinical Application, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan.
authors.forename: A N
authors.initials: AN
authors.lastname: Balamurugan
authors.forename: Yuanjun
authors.initials: Y
authors.lastname: Gu
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Tabata
authors.forename: Masaaki
authors.initials: M
authors.lastname: Miyamoto
authors.forename: Wanxing
authors.initials: W
authors.lastname: Cui
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hori
authors.forename: Akira
authors.initials: A
authors.lastname: Satake
authors.forename: Natsuki
authors.initials: N
authors.lastname: Nagata
authors.forename: Wenjing
authors.initials: W
authors.lastname: Wang
authors.forename: Kazutomo
authors.initials: K
authors.lastname: Inoue
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight; D002470:Cell Survival / Q000187:drug effects; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005951:Glucose Tolerance Test; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D008297:Male; D018919:Neovascularization, Physiologic; D010179:Pancreas / Q000098:blood supply* / Q000187:drug effects; D019397:Pancreas, Artificial; D011093:Polyethylene Terephthalates; D051381:Rats; D011917:Rats, Inbred Lew; D017207:Rats, Sprague-Dawley
chemical_list: D001786:Blood Glucose; D011093:Polyethylene Terephthalates; D016222:Fibroblast Growth Factor 2