In vitro release and antibacterial activity of poly (oleic/linoleic acid dimer: sebacic acid)-gentamicin.
OBJECTIVE
To investigate whether poly (oleic/linoleic acid dimer: sebacic acid)-getamicin [Poly(OAD/LOAD:SA)-gentamicin] delivery system was useful to treat chronic osteomyelitis.


METHODS
Drug delivery system consisted of gentamicin sufate dispersed in a copolymer containing oleic/linoleic acid dimer (OAD/LOAD) and sebacic acid (SA) in a 1:1 weight ratio. The gentamicin release from [Poly(OAD/LOAD:SA)-gentamicin] was tested in water, 0.9 % saline, and phosphate buffer 0.1 mol/L.


RESULTS
The gentamicin concentration peak was found on d 2, then slowly decreased, considerable amount of gentamicin was still released on d 50. From d 2 to d 50, the gentamicin concentration in the releasing fluids was from 59 to 42128-fold and 1.8 to 1314-fold of the MIC for Staphylococcus aureus and Escherichia coli, respectively. Staphylococcus aureus and Escherichia coli were strongly inhibited by the releasing fluids for 50 d. The gentamicin release and anti-bacterial activity in the three media were similar, only in 0.1 mol/L phosphate buffer, from d 2 to d 14 it was lower.


CONCLUSIONS
Poly(OAD/LOAD:SA)-gentamicin was useful to treat chronic osteomyelitis.
Yang
Xiu-Fen
XF
Department of Clinical Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. xiufenyang@163.net
Zeng
Fan-Dian
FD
Zhou
Zhi-Bin
ZB
Huang
Kai-Xun
KX
Xu
Hui-Bi
HB
D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D003652:Decanoic Acids / Q000737:chemistry; D003692:Delayed-Action Preparations; D003998:Dicarboxylic Acids / Q000737:chemistry; D016503:Drug Delivery Systems; D004926:Escherichia coli / Q000187:drug effects*; D005839:Gentamicins / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D019787:Linoleic Acid / Q000737:chemistry; D019301:Oleic Acid / Q000737:chemistry; D011108:Polymers; D013211:Staphylococcus aureus / Q000187:drug effects*
D000900:Anti-Bacterial Agents; D003652:Decanoic Acids; D003692:Delayed-Action Preparations; D003998:Dicarboxylic Acids; D005839:Gentamicins; D011108:Polymers; D019301:Oleic Acid; C011107:sebacic acid; D019787:Linoleic Acid