title: New approach for the detection of BSH and its metabolites using capillary electrophoresis and electrospray ionization mass spectrometry.
abstract: Boron neutron capture therapy is a promising binary treatment for cancer. It is based on the nuclear fission that occurs when non-radioactive 10B absorbs thermal neutrons. One of the two boron compounds currently used in clinical trials for this therapy is BSH. To ensure differentiated retention in the tumour versus normal tissue prior to treatment, routine analytical methods to determine pharmacokinetics must be available. For this purpose we have developed a new, easy and time saving approach, in which the separation of boron derivatives is performed by means of capillary electrophoresis (CE). The CE method allows analyses to be performed in short times (less than 18 min), sensitively (LOD 8 pg loaded on the capillary) quantitatively (LOQ 5 microg/ml) and with a high efficiency of separation. Moreover it is simpler than HPLC and more reproducible (intra- and inter-day values were +/-1% and +/-3%, respectively), and does not require a specific column of derivatization. Mass spectrometry analysis of boron derivatives in different samples was also performed to ensure correct attribution of the CE peaks.
authors.affiliation: Istituto Tecnologie Biomediche-CNR, Via Fratelli Cervi, 93, 20090 Segrate, Milan, Italy. mauri@itba.mi.cnr.it
authors.forename: Pier Luigi
authors.initials: PL
authors.lastname: Mauri
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Basilico
authors.forename: Pier Giorgio
authors.initials: PG
authors.lastname: Pietta
authors.forename: Erica
authors.initials: E
authors.lastname: Pasini
authors.forename: Diego
authors.initials: D
authors.lastname: Monti
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Sauerwein
mesh_terms: D001896:Boron Compounds / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D016754:Boron Neutron Capture Therapy; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D001896:Boron Compounds