Snapin interacts with the N-terminus of regulator of G protein signaling 7.
The N-terminus of regulator of G protein signaling 7 (RGS7) contains a dishevelled/egl-10/pleckstrin (DEP) domain of unknown function. To gain insight into its function, we used yeast two-hybrid analysis to screen a human whole brain cDNA library in order to identify proteins that interact specifically with the N-terminus of human RGS7 (amino acid residues 1-248). From this analysis, we identified snapin, a protein associated with the SNARE complex in neurons, as an interactor with the N-terminus of RGS7. Deletion mutation analysis in yeast demonstrated that the interaction between RGS7 and snapin is specific and is mediated primarily by amino acid residues 1-69 of RGS7 (which contains the proximal portion of the DEP domain). The interaction between RGS7 and snapin was also demonstrated in mammalian cells by coimmunoprecipitation and pull-down assays. Our results suggest that RGS7 could play a role in synaptic vesicle exocytosis through its interaction with snapin.
Hunt
Rachel A
RA
Neuroscience Discovery Research, Wyeth Research, Princeton, NJ 08543-8000, USA.
Edris
Wade
W
Chanda
Pranab K
PK
Nieuwenhuijsen
Bart
B
Young
Kathleen H
KH
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D016466:CHO Cells; D002352:Carrier Proteins / Q000378:metabolism*; D003001:Cloning, Molecular; D006224:Cricetinae; D017931:DNA Primers; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000502:physiology*; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D020710:RGS Proteins / Q000737:chemistry / Q000502:physiology*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D015398:Signal Transduction / Q000502:physiology*; D014162:Transfection; D033921:Vesicular Transport Proteins
D002352:Carrier Proteins; D017931:DNA Primers; D008565:Membrane Proteins; D009479:Neuropeptides; D010446:Peptide Fragments; D020710:RGS Proteins; C492039:RGS7 protein, human; D011994:Recombinant Proteins; C118519:SNAPIN protein, human; D033921:Vesicular Transport Proteins; D019204:GTP-Binding Proteins