title: Random amplified polymorphic DNA analysis of DNA extracted from Trichuris trichiura (Linnaeus, 1771) eggs and its prospective application to paleoparasitological studies.
abstract: Random amplified polymorphic DNA analysis was applied to DNAs extracted from Trichuris trichiura eggs recovered from human fecal samples. Four out of 6 primers tested displayed 18 distinct and well defined polymorphic patterns, ranging from 650 to 3200 base pairs. These results, upon retrieval and DNA sequencing of some of these bands from agarose gels, might help in establishing. T. trichiura specific genetic markers, not available yet, and an important step to design primers to be used in molecular diagnosis approaches.
authors.affiliation: Laboratório de Paleoparasitologia Molecular Eduardo Marques, Departamento de Endemias Samuel Pessoa, Escola Nacional de Saúde Pública-Fiocruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Elaine Machado
authors.initials: EM
authors.lastname: Martinez
authors.forename: Jorge Antonio Santos
authors.initials: JA
authors.lastname: Correia
authors.forename: Erika Verissimo
authors.initials: EV
authors.lastname: Villela
authors.forename: Antonio Nascimento
authors.initials: AN
authors.lastname: Duarte
authors.forename: Luiz Fernando
authors.initials: LF
authors.lastname: Ferreira
authors.forename: Alexandre Ribeiro
authors.initials: AR
authors.lastname: Bello
mesh_terms: D000818:Animals; D001483:Base Sequence; D018509:DNA, Helminth / Q000235:genetics / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D005819:Genetic Markers; D006801:Humans; D008969:Molecular Sequence Data; D010063:Ovum; D019105:Random Amplified Polymorphic DNA Technique; D014258:Trichuris / Q000235:genetics*
chemical_list: D018509:DNA, Helminth; D005819:Genetic Markers