[Leishmania major lipophosphoglycan modulates the expression of receptors involved in parasite internalization in skin Langerhans cells].
Despite the immunological changes recognized to be produced during Leishmania infection and the central role played by Langerhans cells, it is not known whether Leishmaina lipophosphoglycan, the most abundant glycolipid on the parasite surface, affects the functions of Langerhans cells. Here, we provide evidence that exposure of Langerhans cells to Leishmaina (L.) major lipophosphoglycan has consequences for the expression of surface receptors. Down-regulation of receptors involved in host cell-parasite interaction are observed after 4 h exposure of Langerhans cells to lipophosphoglycan. Many of the changes are also induced in Langerhans cells incubated with L. major-conditioned medium, indicating that the observed effects may be mediated by soluble factors released by the parasite into the culture, as it is the case for the carbohydrate moiety of lipophosphoglycan. Taken together, these results indicate that the changes in surface molecule expression induced by the exposure of Langerhans cells to lipophosphoglycan might reflect changes in their signalling functions from the infected skin.
Ponte-Sucre
Alicia
A
Laboratorio de Fisiología Molecular, Instituto de Medicina Experimental, Facultad de Medicina, Universidad Central de Venezuela, Apdo. 68256, Caracas 1062-A, Venezuela. aiponte@reacciun.ve
Scharner
Anabel
A
Moll
Heidrun
H
D000818:Animals; D000954:Antigens, Surface / Q000187:drug effects* / Q000276:immunology; D005434:Flow Cytometry; D006028:Glycosphingolipids / Q000302:isolation & purification / Q000494:pharmacology*; D007801:Langerhans Cells / Q000187:drug effects* / Q000276:immunology / Q000469:parasitology; D018320:Leishmania major / Q000737:chemistry* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C
D000954:Antigens, Surface; D006028:Glycosphingolipids; C008290:lipophosphonoglycan