title: The genomic organization of the murine Mlc1 (Wkl1, KIAA0027) gene.
abstract: The human MLC1( WKL1, KIAA0027) gene encodes a putative transmembrane protein expressed exclusively in brain. Recessive mutations within this gene cause megalencephalic leukoencephalopathy with subcortical cysts (MLC, MIM 604004, 605908). Furthermore, a missense mutation in this gene is suggestively linked with hereditary catatonic schizophrenia in a large pedigree. The murine gene Mlc1is composed of 12 exons spanning approximately 20 kb, and all exon-intron boundaries conform to the GT/AG consensus. The single copy transcript after splicing is approximately 2.8 kb in length, it contains 496 bp of 5' untranslated region (5'-UTR) and 1143 bp of 3'-UTR, and encodes a protein of 382 amino acids. Potential binding sites for transcription factors including CCAAT-boxes are present in the 5'-flanking region. Fluorescent in situ hybridization localizes the gene to mouse chromosome 15E-F, a region syntenic to human chromosome 22q13. The characterization of the genomic structure of the murine gene will facilitate studies of gene function and physiological properties of the encoded protein in transgenic mouse models.
authors.affiliation: Department of Psychiatry and Psychotherapy, University of Würzburg, Germany.
authors.forename: V
authors.initials: V
authors.lastname: Steinke
authors.forename: J
authors.initials: J
authors.lastname: Meyer
authors.forename: Y V
authors.initials: YV
authors.lastname: Syagailo
authors.forename: G
authors.initials: G
authors.lastname: Ortega
authors.forename: H
authors.initials: H
authors.lastname: Hameister
authors.forename: R
authors.initials: R
authors.lastname: Mössner
authors.forename: A
authors.initials: A
authors.lastname: Schmitt
authors.forename: K-P
authors.initials: KP
authors.lastname: Lesch
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005091:Exons / Q000235:genetics; D005786:Gene Expression Regulation / Q000235:genetics; D006801:Humans; D007438:Introns / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA / Q000379:methods; D017386:Sequence Homology, Amino Acid
chemical_list: C425069:MLC1 protein, human; D008565:Membrane Proteins; C498927:Mlc1 protein, mouse