Evaluation of the BDProbeTec ET system for direct detection of Mycobacterium tuberculosis in pulmonary and extrapulmonary samples: a multicenter study.
We evaluated the BDProbeTec ET system (Becton Dickinson, Sparks, Md.), a strand displacement amplification-based technique, for direct detection of Mycobacterium tuberculosis in 867 clinical samples. Of 294 extrapulmonary specimens, 52 had positive results by both BDProbeTec ET and culture and 209 had negative results by both methods; sensitivity and specificity were 76.5 and 95.9%, respectively. After resolution of discrepancies, the sensitivity rose to 77.8%.
Mazzarelli
Gianna
G
Microbiology and Virology Laboratory, Regional Reference Center for Mycobacteria, Careggi Hospital, Florence, Italy.
Rindi
Laura
L
Piccoli
Paola
P
Scarparo
Claudio
C
Garzelli
Carlo
C
Tortoli
Enrico
E
D001826:Body Fluids / Q000382:microbiology; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012137:Respiratory System / Q000382:microbiology; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000382:microbiology*; D014397:Tuberculosis, Pulmonary / Q000382:microbiology*
D003470:Culture Media; D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic