The endomembrane requirement for cell surface repair.
The capacity to reseal a plasma membrane disruption rapidly is required for cell survival in many physiological environments. Intracellular membrane (endomembrane) is thought to play a central role in the rapid resealing response. We here directly compare the resealing response of a cell that lacks endomembrane, the red blood cell, with that of several nucleated cells possessing an abundant endomembrane compartment. RBC membrane disruptions inflicted by a mode-locked Ti:sapphire laser, even those initially smaller than hemoglobin, failed to reseal rapidly. By contrast, much larger laser-induced disruptions made in sea urchin eggs, fibroblasts, and neurons exhibited rapid, Ca(2+)-dependent resealing. We conclude that rapid resealing is not mediated by simple physiochemical mechanisms; endomembrane is required.
McNeil
Paul L
PL
Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta, GA 30912, USA. pmcneil@mail.mcg.edu
Miyake
Katsuya
K
Vogel
Steven S
SS
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002462:Cell Membrane / Q000502:physiology*; D002463:Cell Membrane Permeability; D002470:Cell Survival / Q000502:physiology; D004912:Erythrocytes / Q000166:cytology / Q000502:physiology; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D005456:Fluorescent Dyes; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000502:physiology*; D008854:Microscopy, Electron; D009474:Neurons / Q000166:cytology / Q000502:physiology; D009865:Oocytes / Q000166:cytology / Q000502:physiology; D009997:Osmotic Pressure; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds; D051381:Rats; D012617:Sea Urchins; D013314:Stress, Mechanical
C073804:FM1 43; D005456:Fluorescent Dyes; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds; D002118:Calcium