title: Simple luminescence method for estimation of benzo[a]pyrene in a complex mixture of polycyclic aromatic hydrocarbons without a pre-separation procedure.
abstract: Benzo[a]pyrene causes cancer at cellular level and is widely present in the environment. Conventional spectroscopic methods for analysis of this compound need a pre-separation procedure due to severe spectral overlap from other polycyclic aromatic hydrocarbons. We report a simple method that avoids spectral overlap of benzo[a]pyrene from other impurities or polycyclic aromatic hydrocarbons (PAHs), thus it can easily identify benzo[a]pyrene in a complex PAH mixture. The method could easily identify benzo[a]pyrene in an 18-component PAH mixture. Calibration plots in methanol solution and in micellar media show a good linearity (R > 0.9997) in the benzo[a]pyrene concentration range generally found in the environment. The method gives a detection limit of 1.52 x 10(-9) mol/L in CTAB micellar medium and 2.55 x 10(-9) mol/L in methanol solution. The proposed method is selective, sensitive and fast. The fluorescence response of benzo[a]pyrene is found to be a potential candidate to sense the critical micellar concentration (CMC) of CTAB micelles.
authors.affiliation: Department of Chemistry, Indian Institute of Technology-Madras, Chennai 600 036, India. digpatra@hotmail.com
authors.forename: Digambara
authors.initials: D
authors.lastname: Patra
mesh_terms: D000465:Algorithms; D001564:Benzo(a)pyrene / Q000032:analysis*; D000077286:Cetrimonium; D002593:Cetrimonium Compounds; D045424:Complex Mixtures / Q000032:analysis; D004785:Environmental Pollutants / Q000032:analysis*; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D000432:Methanol; D008823:Micelles; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis*; D012906:Smoke / Q000032:analysis; D012997:Solvents; D013050:Spectrometry, Fluorescence; D013501:Surface-Active Agents; D014026:Nicotiana / Q000737:chemistry
chemical_list: D002593:Cetrimonium Compounds; D045424:Complex Mixtures; D004785:Environmental Pollutants; D007202:Indicators and Reagents; D008823:Micelles; D011084:Polycyclic Aromatic Hydrocarbons; D012906:Smoke; D012997:Solvents; D013501:Surface-Active Agents; D001564:Benzo(a)pyrene; D000432:Methanol; D000077286:Cetrimonium