title: Trichomonas vaginalis: identification of a phospholipase A-dependent hemolytic activity in a vesicular subcellular fraction.
abstract: Trichomonad total extracts (TTE), or vesicular (P30) and soluble (530) subcellular fractions from 3 pathogenic Trichomonas vaginalis strains (GT-3. GT-13. and GT-15), lysed both human and Sprague-Dawley rat erythrocytes in a time- and dose-dependent manner. The entire hemolytic activity of TTE was located in P30, showing 2 peaks of maximum activity, one at pH 6.0 and another at pH 8.0. in the presence of 1 mM Ca2+. Hemolytic activity on rat erythrocytes was greater at pH 6.0 16.71 +/- 0.33 hemolytic units IHU]/mg/hr to 11.60 +/- 0.24 HU/mg/hr) than at pH 8.0 (3.81 +/- 0.30 HU/mg/hr to 5.75 +/- 0.65 HU/mg/hr). and it was greater than that on human red blood cells at pH 6.0 (2.67 +/- 0.19 HU/mg/hr to 4.08 +/- 0.15 HU/mg/hr) or pH 8.0 (2.24 +/- 0.0 9 HU/mg/hr to 2.81 +/- 0.06 HU/mg/hr). The alkaline and acidic hemolytic activity diminished (60-93% at pH 6.0 and 78-93% at pH 8.0) by the effect of 80 microM Rosenthal's inhibitor, which also inhibited 27-45% and 29-54% trichomonad alkaline and acidic phospholipase A activities, respectively. Vesicles, vacuoles, and hydrogenosomes were rich in P30. Trichomonas vaginalis has a hemolytic PLA, which could be involved in its cytopathogenic mechanism.
authors.affiliation: División de Biología Celular y Molecular, Centro de Investigación Biomédica del Noreste, Instituto Mexicano del Seguro Social, Administración de Correos No. 4, Apartado Postal 020-E, Colonia Independencia, Monterrey, CP 64720, Nuevo León, Mexico.
authors.forename: Javier
authors.initials: J
authors.lastname: Vargas-Villarreal
authors.forename: Benito D
authors.initials: BD
authors.lastname: Mata-Cárdenas
authors.forename: Francisco
authors.initials: F
authors.lastname: González-Salazar
authors.forename: Hector G
authors.initials: HG
authors.lastname: Lozano-Garza
authors.forename: Elva I
authors.initials: EI
authors.lastname: Cortes-Gutierrez
authors.forename: Rebeca
authors.initials: R
authors.lastname: Palaclos-Corona
authors.forename: Herminia G
authors.initials: HG
authors.lastname: Martínez-Rodríguez
authors.forename: Enrique
authors.initials: E
authors.lastname: Ramírez-Bon
authors.forename: Salvador
authors.initials: S
authors.lastname: Said-Fernández
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000378:metabolism*; D006461:Hemolysis / Q000502:physiology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010741:Phospholipases A / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051381:Rats; D013228:Stearates / Q000494:pharmacology; D014246:Trichomonas vaginalis / Q000201:enzymology / Q000378:metabolism* / Q000472:pathogenicity; D014774:Virulence
chemical_list: D013228:Stearates; C015161:Rosenthal's inhibitor; D010741:Phospholipases A; D002118:Calcium