Quantitative evaluation of DNA fragmentation.
A new method for evaluation of DNA fragmentation in tissue is based on computer-aided densitometric standardization of the sum of optical densities of DNA fragments visualized by electrophoresis (180-2500 b. p.) in comparison with the density of high molecular weight DNA in the same sample. The method allows evaluation of DNA fragmentation, an acknowledged marker of apoptosis, in the fetal rat brain and detection of increased DNA fragmentation during stress and under the effect of glucocorticoids.
Kalinina
T S
TS
Laboratory of Genetic Basis of Neuroendocrine Regulation, Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia. kalin@bionet.nsc.ru
Bannova
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AV
Dygalo
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D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D001921:Brain / Q000187:drug effects / Q000196:embryology; D053938:DNA Fragmentation / Q000187:drug effects / Q000502:physiology*; D003720:Densitometry; D005260:Female; D006854:Hydrocortisone / Q000494:pharmacology; D007091:Image Processing, Computer-Assisted; D008970:Molecular Weight; D011247:Pregnancy; D051381:Rats; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological / Q000473:pathology
D000893:Anti-Inflammatory Agents; D012965:Sodium Chloride; D006854:Hydrocortisone