title: Prevention of intraperitoneal adhesion formation using beta-glucan after ileocolic anastomosis in a rat bacterial peritonitis model.
abstract: BACKGROUND
  To investigate the effects of beta-glucan on intraabdominal abscess and adhesion formation after ileocolic anastomosis in a rat bacterial peritonitis model.
  
  
  METHODS
  Sixty male Wistar rats were used in this study. Bacterial peritonitis was induced by performing a cecal ligation and puncture (CLP). On the first day, abdomen was reopened and peritoneal fluid samples were taken for microbiological examination. Thereafter, cecum was resected and ileocolic anastomosis was made. Group 1 rats were given 1 mL of normal saline as a placebo. Group 2 and group 3 rats were given beta-D-glucan 2 mg/kg by intramuscularly; 1 mg of beta-1,3-D-glucanase was administered to group 3 rats just after the use of beta-D-glucan. Half of each group were killed at day 7 and at day 21, respectively. Adhesions were scored and the presence of intraabdominal abscesses was noted.
  
  
  RESULTS
  One day after CLP, microbiological examination showed polymicrobial bacterial peritonitis. Five (8%) of the 60 animals died owing to sepsis. One week after CLP, in two rats in each group developed abscess formation. Three weeks after CLP, abscess formation was observed in only one rat in each group. The rats treated with the beta-glucan had significantly lower adhesion scores than did the saline-treated rats (P = 0.008 at one week; P = 0.001 at 3 weeks). Administration of beta-glucanase inhibited beta-glucan activity and resulted in more adhesions (P = 0.022 at 1 week; P = 0.006 at 3 weeks).
  
  
  CONCLUSIONS
  Although the use of beta-glucan after ileocolic anastomosis in rats with experimentally developed intraabdominal sepsis does not have any significantly effect on mortality and abscess formation, beta-glucan is capable of reducing the frequency of adhesion. This effect of beta-glucan has been prevented with beta-glucanase
authors.affiliation: Department of General Surgery, University of Erciyes, School of Medicine, 38039, Kayseri, Turkey. bedirli@erciyes.edu.tr
authors.forename: Abdulkadir
authors.initials: A
authors.lastname: Bedirli
authors.forename: Selma
authors.initials: S
authors.lastname: Gokahmetoglu
authors.forename: Omer
authors.initials: O
authors.lastname: Sakrak
authors.forename: Nail
authors.initials: N
authors.lastname: Ersoz
authors.forename: Demet
authors.initials: D
authors.lastname: Ayangil
authors.forename: Hüseyin
authors.initials: H
authors.lastname: Esin
mesh_terms: D018784:Abdominal Abscess / Q000209:etiology / Q000517:prevention & control*; D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000714:Anastomosis, Surgical / Q000009:adverse effects*; D000818:Animals; D005936:Glucans / Q000494:pharmacology*; D006026:Glycoside Hydrolases / Q000494:pharmacology; D007422:Intestines / Q000601:surgery; D008297:Male; D023421:Models, Animal; D010529:Peritoneal Cavity; D010538:Peritonitis / Q000209:etiology / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar; D000267:Tissue Adhesions / Q000209:etiology / Q000517:prevention & control*
chemical_list: D000276:Adjuvants, Immunologic; D005936:Glucans; D006026:Glycoside Hydrolases