title: Effect of distraction rate and consolidation period on bone density following mandibular osteodistraction in rats.
abstract: The high cost of large animal protocols has limited the study of distraction osteogenesis (DO) in the craniofacial region. This study was designed to characterise a rat model for DO with regard to distraction rate and consolidation period. Unilateral mandibular distraction was performed on 129 male Sprague-Dawley rats using an osteotomy from the sigmoid notch to the inferior border of mandible. After a 3-day latency, 12 groups of 8-9 rats underwent distraction for 5 days at four different rates (0, 0.2, 0.4, 0.6mm per day), with three different post-osteotomy sacrifice times (10, 24, and 38 days) and four final predicted distraction lengths (0, 1, 2, and 3mm). Another four groups of rats (N=8 per group) were sacrificed 6 days post-osteotomy, resulting in distraction for 3 days with a predicted distraction length of 0, 0.6, 1.2, 1.8mm. Changes in mandibular morphology were measured from radiographs of disarticluated hemimandibles. The bone density of the regenerate and control sites was measured using microdensitometry calibrated with an epoxy stepwedge. Distraction linearly increased mandibular length, distraction gap width and the area of the distraction gap (P<0.00005). Mandibular length increased by 0.394 mm per distraction rate. Gap width and area increased by 0.67 and 5.8mm(2) per distraction rate, respectively. The increase in length represents only 39.4% of what was predicted, suggesting that compensatory alteration in condylar or mandibular morphology may have occurred. This speculation was further supported by the finding that mandibular length, measured without the condylar landmark, was 53.8% of predicted. During DO and early consolidation, the measures of bone density in the regenerates decreased compared to control for all groups. Thereafter, bone density in the regenerates generally increased in all groups until day 24 (P<0.01), obtaining levels that were comparable to the unoperated side. At both rostral and caudal sites adjacent to the osteotomies, measures of bone density were enhanced over control in all groups, with the rostral site also showing significant increases over time in the sham and the highest distraction groups (P<0.008 and P<0.014). We conclude that this rat model for mandibular distraction osteogenesis provides bone density changes that are consistent with those reported using larger animal protocols.
authors.affiliation: Department of Orthodontics, University of Washington School of Dentistry, Box 357446, Seattle, WA 98195-3446, USA. gking@u.washington.edu
authors.forename: G J
authors.initials: GJ
authors.lastname: King
authors.forename: Z J
authors.initials: ZJ
authors.lastname: Liu
authors.forename: L L
authors.initials: LL
authors.lastname: Wang
authors.forename: I Y
authors.initials: IY
authors.lastname: Chiu
authors.forename: M F
authors.initials: MF
authors.lastname: Whelan
authors.forename: G J
authors.initials: GJ
authors.lastname: Huang
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015519:Bone Density; D001861:Bone Regeneration; D004195:Disease Models, Animal; D008297:Male; D008334:Mandible / Q000473:pathology / Q000503:physiopathology*; D019857:Osteogenesis, Distraction / Q000379:methods*; D010027:Osteotomy / Q000379:methods; D011184:Postoperative Period; D051381:Rats; D017207:Rats, Sprague-Dawley