EphB4 receptor tyrosine kinase transgenic mice develop glomerulopathies reminiscent of aglomerular vascular shunts.
We have established transgenic mice over-expressing the EphB4 receptor tyrosine kinase in the kidney. The EphB4 protein was localised to the developing tubular system of both control and transgenic newborn mice. In transgenic adults, transgene expression persisted in the proximal tubules and the Bowman's capsules, structures, which were not stained in control kidneys. The glomeruli of control animals consisted of regular, round vascular baskets with clearly discernable afferent and efferent arterioles. In contrast, approximately 40% of the transgenic glomeruli had an irregular shrivelled appearance and many exhibited fused, horse shoe-like afferent and efferent arterioles bypassing the glomerulus. These abnormal glomerular structures are very reminiscent of aglomerular vascular shunts, a human degenerative glomerulopathy of unknown aetiology.
Andres
Anne-Catherine
AC
The Tiefenau Laboratory, Department of Clinical Research, University of Berne, Tiefenaustrasse 120, CH-3004 Berne, Switzerland.
Munarini
Nadia
N
Djonov
Valentin
V
Bruneau
Salomé
S
Zuercher
Gisela
G
Loercher
Saemi
S
Rohrbach
Valeria
V
Ziemiecki
Andrew
A
D000818:Animals; D018076:DNA, Complementary / Q000378:metabolism; D004195:Disease Models, Animal; D036388:Ephrin-B2 / Q000235:genetics; D005921:Glomerulonephritis / Q000235:genetics*; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism; D007674:Kidney Diseases / Q000235:genetics* / Q000473:pathology; D007678:Kidney Glomerulus / Q000473:pathology; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D008855:Microscopy, Electron, Scanning; D009389:Neovascularization, Pathologic; D010536:Peritoneovenous Shunt; D010641:Phenotype; D011401:Promoter Regions, Genetic; D012313:RNA / Q000378:metabolism; D036224:Receptor, EphB4 / Q000235:genetics* / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
D018076:DNA, Complementary; D036388:Ephrin-B2; D012313:RNA; D036224:Receptor, EphB4