[Effect of inductors and inhibitors of the mitochondrial permeability transition pore on its opening and release of unidentified mitochondrial factor].
The release of an unidentified substance (factor) induced by sulfhydryl group (SH) modifier and mitochondrial permeability transition pore (MPT) inductor phenylarsine oxide (PAO) has been found in vitro on isolated guinea-pig and rat heart mitochondria. The factor was also released at oxidative stress. The factor release induced by PAO was inhibited by cyclosporin A (CsA), the MPT inhibitor. Protective actions of antioxidants melatonin and trolox in vitro and in vivo, as well as dithiothreitol (DTT) and diethylmaleat (DEM) were studied. The influences of nitric oxide (NO) donor, sodium nitroprusside (SNP) on the mitochondrial factor release were also studied. We have shown the participation of this agent in the regulation of factor formation after inhibition of NO-synthase activity (NOS) by aminoguanidine. The data were obtained about MPT-opening after Ca2+ loading and under the influence of the MPT inductor PAO, inhibited by CsA. The results obtained on isolated mitochondria are in a good agreement with the those on the isolated guinea-pig heart after myocardial ischemia-reperfusion. It was concluded that mitochondria are essential for the factor formation and its release under ischemia-reperfusion of the myocardium.
Sahach
V F
VF
A.A. Bogomoletz Institute of Physiology, National Academy of Science of Ukraine, Kiev.
Vavilova
H L
HL
Strutyns'ka
N A
NA
Akopova
O V
OV
D000818:Animals; D001152:Arsenicals / Q000494:pharmacology; D016572:Cyclosporine / Q000494:pharmacology; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007473:Ion Channels / Q000819:agonists* / Q000037:antagonists & inhibitors*; D008565:Membrane Proteins / Q000378:metabolism*; D008929:Mitochondria, Heart / Q000378:metabolism*; D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D017202:Myocardial Ischemia / Q000150:complications / Q000378:metabolism / Q000503:physiopathology; D015428:Myocardial Reperfusion Injury / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology; D018384:Oxidative Stress / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar
D001152:Arsenicals; D007473:Ion Channels; D008565:Membrane Proteins; D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; C029341:oxophenylarsine; D016572:Cyclosporine