Quantitative analysis of human immunoregulatory cytokines by electrochemiluminescence method.
Quantitative analysis of human immunoregulatory cytokines in physiological media and cell cultures plays an important role in fundamental and clinical research. Here we describe the quantification of interleukin (IL)-2, IL-4, IL-10 and interferon-gamma (IFN-gamma) in human serum and peripheral blood mononuclear cell (PBMC)-conditioned medium by electrochemiluminescence method (ECL). We demonstrate that this approach allows to detect cytokine concentration from 1 pg/ml. The high sensitivity in combination with accuracy and wide range of determined concentration indicates that ECL meets the standards of quantitative analysis of cytokines. Simplicity and short time of procedure, small assay volume and high reproducibility make ECL method competitive in practical use with conventional quantitative methods of cytokine detection.
Sennikov
Sergey V
SV
Research Institute of Clinical Immunology, Siberian Branch of Russian Academy of Medical Science, 14, Yadrintsevskaja str., Novosibirsk, 630091, Russia. ici@online.nsk.su
Krysov
Sergey V
SV
Injelevskaya
Tatiana V
TV
Silkov
Alexandr N
AN
Grishina
Lyubov V
LV
Kozlov
Vladimir A
VA
D000906:Antibodies; D000911:Antibodies, Monoclonal; D017077:Culture Media, Conditioned; D016207:Cytokines / Q000032:analysis* / Q000097:blood / Q000592:standards; D006801:Humans; D007118:Immunoassay / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D007371:Interferon-gamma / Q000032:analysis / Q000097:blood; D016753:Interleukin-10 / Q000032:analysis / Q000097:blood; D007376:Interleukin-2 / Q000032:analysis / Q000097:blood; D015847:Interleukin-4 / Q000032:analysis / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008163:Luminescent Measurements; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000906:Antibodies; D000911:Antibodies, Monoclonal; D017077:Culture Media, Conditioned; D016207:Cytokines; D007376:Interleukin-2; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma