title: [Differential expression of RPL6/Taxreb107 in drug resistant gastric cancer cell line SGC7901/ADR and its correlation with multiple-drug resistance].
abstract: OBJECTIVE
  To investigate the differential expression of RPL6/Taxreb107 between drug-resistant gastric cancer cell line SGC7901/ADR and gastric cancer cell line SGC7901 as well as its correlation with multiple-drug resistance (MDR) in gastric cancer cells.
  
  
  METHODS
  Total RNA was extracted from SGC7901 and SGC77901/ADR, with internal control RT-PCR, Northern blot, gene cloning and expression, construction of eukaryotic expression vector, gene transfection by electroporation. The accumulation and retention of ADR in transiently transfected cell was detected by flow cytometry.
  
  
  RESULTS
  The internal control RT-PCR and Northern blot showed high RPL6/Taxreb107 expression in SGC7901/ADR cell line. Sense and antisense eukaryonic expression vectors demonstrated by double enzyme digestion were successfully transfected into gastric cancer cell line SGC7901 and SGC7901/ADR respectively by electroporation. The accumulation and retention of ADR detected 48 hours after transfection showed that RPL6 gene had shown effect on drug resistance in gastric cancer cell.
  
  
  CONCLUSIONS
  The high expression of RPL6/Taxreb107 in drug resistant gastric cancer cell shows its correlation with multiple-drug resistance in gastric cancer.
authors.affiliation: Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi'an 710033, China.
authors.forename: Jing-ping
authors.initials: JP
authors.lastname: Du
authors.forename: Xiao-hang
authors.initials: XH
authors.lastname: Jin
authors.forename: Yong-quan
authors.initials: YQ
authors.lastname: Shi
authors.forename: Yun-xin
authors.initials: YX
authors.lastname: Cao
authors.forename: Yan-qiu
authors.initials: YQ
authors.lastname: Zhao
authors.forename: Chang-Jiang
authors.initials: CJ
authors.lastname: Liu
authors.forename: Fang
authors.initials: F
authors.lastname: Yin
authors.forename: Wen-hua
authors.initials: WH
authors.lastname: Hu
authors.forename: Bao-jun
authors.initials: BJ
authors.lastname: Chen
authors.forename: Tai-dong
authors.initials: TD
authors.lastname: Qiao
authors.forename: Dai-ming
authors.initials: DM
authors.lastname: Fan
mesh_terms: D004268:DNA-Binding Proteins / Q000378:metabolism*; D018432:Drug Resistance, Multiple / Q000502:physiology*; D019008:Drug Resistance, Neoplasm / Q000502:physiology*; D006801:Humans; D013223:Statistics as Topic; D013274:Stomach Neoplasms / Q000473:pathology; D014407:Tumor Cells, Cultured
chemical_list: D004268:DNA-Binding Proteins; C093544:tax-responsive-element-binding protein 107, mouse