The variation of percent free prostate-specific antigen determined by two different assays.
BACKGROUND
We evaluated the difference in values of the percent free Prostate-specific antigen (PSA) as measured by the Hybritech Tandem R assay (USA) and the Cis bio international assay (France).


METHODS
A total of 99 patients scheduled for transrectal ultrasonography (TRUS) of the prostate and sextant biopsy were subjected to measurements of total and free PSA using the Hybritech Tandem R assay and the Cis bio international assay with the same serum sample.


RESULTS
Thirty patients were confirmed to have prostate cancer and 69 patients to have benign prostate hyperplasia (BPH). The Pearson coefficients of correlation of the Cis bio international assay versus the Tandem R assay were 0.979 for total PSA, 0.987 for free PSA and 0.937 for percent free PSA. The mean total PSA measured by Tandem R was 8.65 +/- 5.36 ng/ml, which was significantly higher than those determined by Cis bio international assay, 7.60 +/- 4.46 ng/ml (p < 0.0001). The mean free PSA determined by Tandem R assay was 1.47 +/- 1.01 ng/ml, which was significantly less than that measured by Cis bio international assay, 1.75 +/- 1.18 ng/ml (p < 0.0001). Thus the percent free PSA calculated by the Cis bio international assay also had significantly higher mean values compared to the Tandem R assay (24.08 +/- 10.09 vs. 18.22 +/- 8.17, p < 0.0001).


CONCLUSIONS
The optimal cut-off value of percent free PSA for biopsy reference was demonstrated to be higher when determined by Cis bio international assay than Tandem R assay.
Yang
Chi-Rei
CR
Division of Urology, Department of Surgery, Taichung Veterans General Hospital, 160 Chung-Kang Road, Section 3, Taichung 407, Taiwan.
Ou
Yen-Chuan
YC
Horng
Yu-Ye
YY
Lee
Hong-Shen
HS
D000230:Adenocarcinoma / Q000097:blood*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D006801:Humans; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D017430:Prostate-Specific Antigen / Q000097:blood*; D011470:Prostatic Hyperplasia / Q000097:blood*; D011471:Prostatic Neoplasms / Q000097:blood*; D011933:Reagent Kits, Diagnostic
D011933:Reagent Kits, Diagnostic; D017430:Prostate-Specific Antigen