title: Emergence of perianal fistulizing disease in the SAMP1/YitFc mouse, a spontaneous model of chronic ileitis.
abstract: OBJECTIVE
  SAMP1/Yit mice spontaneously develop chronic terminal ileitis, reminiscent of the human disease described by Crohn et al. in 1932. Several new phenotypic features have appeared in our colony after more than 20 generations of brother-sister mating. In this report, we describe the distinguishing features of the SAMP1/YitFc substrain at the University of Virginia, compared with the Japanese SAMP1/Yit parental strain.
  
  
  METHODS
  A colony of SAMP1/Yit mice was established at the University of Virginia in 1996, from 2 breeding pairs obtained from Japan. A systematic characterization of their phenotypic and immunologic characteristics was performed at 4, 10, 40, and more than 60 weeks of age.
  
  
  RESULTS
  The following differences were observed: (1) SAMP1/YitFc mice displayed established ileitis as early as 10 weeks of age, (2) the incidence of skin lesions inversely correlated with the occurrence of intestinal inflammation, (3) mice develop chronic ileitis with prominent muscular hypertrophy and focal collagen deposition in inflamed segments, (4) mesenteric lymph node lymphocytes acquired an activated phenotype coincident with disease progression, (5) high interferon-gamma production was detected by 4 weeks of age and preceded the onset of ileitis, and (6) a subgroup of mice (approximately 5%) developed perianal disease with ulceration and fistulae.
  
  
  CONCLUSIONS
  The SAMP1/YitFc substrain exhibits unique characteristics when compared with the original Japanese strain. Of particular interest is the emergence of perianal fistulizing disease, to our knowledge the first report of such occurrence in an animal model of inflammatory bowel disease.
authors.affiliation: Digestive Health Center of Excellence, University of Virginia, Charlottesville, Virginia 22908, USA.
authors.forename: Jesús
authors.initials: J
authors.lastname: Rivera-Nieves
authors.forename: Giorgos
authors.initials: G
authors.lastname: Bamias
authors.forename: Alda
authors.initials: A
authors.lastname: Vidrich
authors.forename: Marco
authors.initials: M
authors.lastname: Marini
authors.forename: Theresa T
authors.initials: TT
authors.lastname: Pizarro
authors.forename: Marcia J
authors.initials: MJ
authors.lastname: McDuffie
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Moskaluk
authors.forename: Steven M
authors.initials: SM
authors.lastname: Cohn
authors.forename: Fabio
authors.initials: F
authors.lastname: Cominelli
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002908:Chronic Disease; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005434:Flow Cytometry; D006984:Hypertrophy; D007079:Ileitis / Q000235:genetics* / Q000473:pathology*; D007082:Ileum / Q000473:pathology; D008198:Lymph Nodes / Q000166:cytology / Q000378:metabolism; D008297:Male; D008640:Mesenteric Lymphadenitis / Q000235:genetics / Q000473:pathology; D051379:Mice; D008817:Mice, Mutant Strains; D009130:Muscle, Smooth / Q000473:pathology; D010481:Perianal Glands / Q000473:pathology*; D010641:Phenotype; D011247:Pregnancy; D012003:Rectal Fistula / Q000235:genetics* / Q000473:pathology*; D012867:Skin / Q000473:pathology; D013047:Specific Pathogen-Free Organisms
chemical_list: D016207:Cytokines