[The mechanism of the supercoiled DNA recognition by eukaryotic type I topoisomerases. I. The enzyme interaction with nonspecific oligonucleotides].
Interaction of the DNA type I topoisomerases from the murine and human placenta cells with nonspecific oligonucleotides was analyzed. The contributions of strong and week nonspecific electrostatic, van der Waals's, and hydrophobic interactions, and hydrogen bonding of the enzymes to the complex formation with the single- and double-stranded DNAs were determined. The factors that determine the top-priority recognition of the topologically stressed DNA were revealed. The results were interpreted in comparison with the X-ray analysis data for human DNA topoisomerase I.
Bugreev
D V
DV
Novosibirsk Institute of Bioorganic Chemistry, Siberian Division, Russian Academy of Sciences, pr. Akademika Lavrent'eva 8, Novosibirsk, 630090 Russia.
Buneva
V N
VN
Sinitsina
O I
OI
Nevinskiĭ
G A
GA
D000818:Animals; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D004264:DNA Topoisomerases, Type I / Q000737:chemistry* / Q000378:metabolism*; D005057:Eukaryotic Cells / Q000502:physiology; D005260:Female; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D051379:Mice; D009690:Nucleic Acid Conformation; D009692:Nucleic Acid Heteroduplexes / Q000378:metabolism; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism*; D010920:Placenta; D011247:Pregnancy; D055672:Static Electricity; D013329:Structure-Activity Relationship
D009692:Nucleic Acid Heteroduplexes; D009841:Oligonucleotides; D004247:DNA; D004264:DNA Topoisomerases, Type I
Mekhanizm uznavaniia superskruchennoĭ DNK éukarioticheskimi topoizomerazami pervogo tipa. I. Vzaimodeĭstvie fermentov s nespetsificheskimi oligonukleotidami.