title: Methylation patterns and K-ras mutations in tumor and paired serum of resected non-small-cell lung cancer patients.
abstract: Gene methylation and K-ras mutations were examined in tumor and paired serum DNA of 50 resected non-small-cell lung cancer patients. RASSF1A, death associated protein kinase and target of methylation-induced silencing were methylated in 17/50 (34%), 23/50 (45%) and 18/50 (35%) tumors, respectively, and in 17/50 (34%), 20/50 (40%) and 17/50 (34%) sera, respectively. Methylation in tumor and serum were closely correlated (P=0.001), but no correlation was found with survival. Twelve K-ras mutations (cysteine) were found in serum and nine mutations were found in tumor (five cysteine, one alanine, one aspartic, one arginine, and one valine). K-ras mutations in serum correlated significantly with survival (P=0.01).
authors.affiliation: Medical Oncology Service, Hospital Germans Trias i Pujol, Ctra Canyet, s/n 08916, Barcelona, Spain.
authors.forename: Jose Luis
authors.initials: JL
authors.lastname: Ramirez
authors.forename: Carme
authors.initials: C
authors.lastname: Sarries
authors.forename: Pedro Lopez
authors.initials: PL
authors.lastname: de Castro
authors.forename: Barbara
authors.initials: B
authors.lastname: Roig
authors.forename: Cristina
authors.initials: C
authors.lastname: Queralt
authors.forename: Daniel
authors.initials: D
authors.lastname: Escuin
authors.forename: Itziar
authors.initials: I
authors.lastname: de Aguirre
authors.forename: Jose Miguel
authors.initials: JM
authors.lastname: Sanchez
authors.forename: Jose Luis
authors.initials: JL
authors.lastname: Manzano
authors.forename: Mireia
authors.initials: M
authors.lastname: Margelí
authors.forename: Jose Javier
authors.initials: JJ
authors.lastname: Sanchez
authors.forename: Julio
authors.initials: J
authors.lastname: Astudillo
authors.forename: Miquel
authors.initials: M
authors.lastname: Taron
authors.forename: Rafael
authors.initials: R
authors.lastname: Rosell
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D051017:Apoptosis Regulatory Proteins; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000097:blood / Q000378:metabolism; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood* / Q000235:genetics*; D003062:Codon; D004247:DNA / Q000378:metabolism; D019175:DNA Methylation; D004252:DNA Mutational Analysis; D064550:Death-Associated Protein Kinases; D005260:Female; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood* / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009154:Mutation; D016133:Polymerase Chain Reaction; D011379:Prognosis; D013997:Time Factors
chemical_list: D051017:Apoptosis Regulatory Proteins; D003062:Codon; D004247:DNA; D064550:Death-Associated Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases