Cloning and characterization of mouse IL-22 binding protein.
Interleukin-22 (IL-22), a member of IL-10 family, plays some important roles in immune response through activation of the STAT 3 signal transduction pathway. Two types of IL-22-binding receptor have been discovered, a membrane-bound receptor and a soluble receptor, both encoded by different genes. IL-22 may be involved in inflammatory processes specifically regulated by soluble receptors. By screening a mouse genomic library for a human IL-22 binding protein homologue, we identified the mouse genomic clone of IL-22 binding protein. Its coding sequence was verified and isolated by RT-PCR. The gene encodes a protein of 230 amino acids that share 67.1% amino-acid sequence identity with human IL-22 binding protein. We designated this receptor 'mouse IL-22 binding protein' (mIL-22BP). mIL-22BP could be upregulated by LPS stimulation in mouse monocytes. mIL-22BP binds to mouse and human IL-22 and neutralizes STAT3 activation induced by both cytokines in human and rat hepatoma cell lines. Treating B cells with mouse IL-22 induces production of reactive oxygen species, which mIL-22BP blocks.
Wei
Chi-Chen
CC
Institute of Basic Medical Sciences, National Cheng Kung University, Tainan, Taiwan.
Ho
T-W
TW
Liang
W-G
WG
Chen
G-Y
GY
Chang
Ming-Shi
MS
D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D006801:Humans; D007378:Interleukins / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D017382:Reactive Oxygen Species / Q000378:metabolism; D018123:Receptors, Interleukin / Q000235:genetics* / Q000378:metabolism; D050796:STAT3 Transcription Factor; D017422:Sequence Analysis, DNA; D015534:Trans-Activators / Q000378:metabolism; D000097043:Interleukin-22
D004268:DNA-Binding Proteins; D007378:Interleukins; D017382:Reactive Oxygen Species; D018123:Receptors, Interleukin; D050796:STAT3 Transcription Factor; D015534:Trans-Activators; D000097043:Interleukin-22; C494086:STAT3 protein, human; C494087:Stat3 protein, mouse; C415369:interleukin-22 receptor