Inhibition of experimental choroidal neovascularization by irsogladine, an anti-gastric ulcer agent.
OBJECTIVE
To determine whether irsogladine inhibits experimental choroidal neovascularization (CNV) induced by laser photocoagulation in pigmented rats.


METHODS
Focal laser photocoagulation (argon green 50 mW, 0.04 s, 200 microm) was applied to the retinochoroid of normal Brown Norway rats. Oral administration of irsogladine (5 mg/kg/day or 50 mg/kg/day) was started 1 week before and continued for 2 weeks after laser photocoagulation. Choroidal vascular casts were made 2 weeks after laser photocoagulation and were examined with a scanning electron microscope (SEM). CNV formation was classified according to three grades and evaluated.


RESULTS
Laser-induced CNV formation was significantly reduced in rats given 5 mg/kg/day (p < 0.01) or 50 mg/kg/day of irsogladine (p < 0.001). Administration of 50 mg/kg/day of irsogladine was more effective in preventing CNV formation than 5 mg/kg/day (p < 0.001). The development of the vascular bud was especially inhibited by 50 mg/kg/day of irsogladine (p < 0.001). CNVs in rats treated with 50 mg/kg/day of irsogladine looked less well developed than those in controls. There was no significant side effect of irsogladine.


CONCLUSIONS
Irsogladine inhibits the development of experimental CNV induced by photocoagulation in pigmented rats.
Kuroki
Akiko Mera
AM
Department of Ophthalmology and Visual Sciences, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan. atkuroki@aol.com
Kitaoka
Takashi
T
Matsuo
Yoko
Y
Amemiya
Tsugio
T
D000818:Animals; D000897:Anti-Ulcer Agents / Q000627:therapeutic use*; D002196:Capillaries / Q000187:drug effects / Q000648:ultrastructure; D002829:Choroid / Q000187:drug effects / Q000648:ultrastructure; D020256:Choroidal Neovascularization / Q000188:drug therapy*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule / Q000662:veterinary; D004353:Drug Evaluation, Preclinical; D017075:Laser Coagulation / Q000379:methods; D008855:Microscopy, Electron, Scanning / Q000295:instrumentation / Q000379:methods; D051381:Rats; D011914:Rats, Inbred BN; D013997:Time Factors; D014227:Triazines / Q000627:therapeutic use*
D000897:Anti-Ulcer Agents; D014227:Triazines; C041836:irsogladine