Elimination of enteric bacteria in biological-chemical wastewater treatment and tertiary filtration units.
The occurrence and removal of salmonellae and faecal indicators in four conventional municipal wastewater treatment plants (MWTP) were investigated. In addition, we tested the efficiency of a semi-technical scale biological nutrient removal process and three pilot-scale tertiary filtration units in microbial removal. All influent samples collected from MWTPs contained salmonellae from 93 to 11,000 MPN/100 ml and indicator bacteria from about 10(7) to 10(8) CFU/100 ml. The reductions in salmonella numbers achieved in full-scale biological-chemical wastewater treatment and semi-technical scale biological nutrient removal processes were usually between 94% and virtually 100% (99.9%) and indicator bacteria reductions between 2 and 3 log units. Microbial numbers in MWTP effluents could be modelled as a function of effluent residual organic matter, suspended solids and total phosphorus concentrations. Pilot-scale tertiary treatment by rapid sand contact filter, chemical contact filter and biological-chemical contact filter reduced salmonella numbers below the detection limit and faecal coliform numbers on average by 99%, 39% and 71%, respectively. A total of 32 Salmonella serovars were identified among 197 Salmonella isolates from municipal wastewaters. Of the isolates, 32% were resistant to nalidixic acid, indicating reduced sensitivity to ciprofloxacin, the drug of choice in the treatment of salmonellosis. In addition, 18% of the isolates were multiresistant. Our results, especially antibiotic resistant Salmonella strains, indicate that conventional municipal wastewater treatment without efficient tertiary treatment, like filtration or disinfection, may constitute a risk for public health.
Koivunen
Jari
J
Department of Environmental Sciences, University of Kuopio, POB 1627, FIN-70211 Kuopio, Finland. jari.koivunen@uku.fi
Siitonen
Anja
A
Heinonen-Tanski
Helvi
H
D000890:Anti-Infective Agents / Q000494:pharmacology; D002939:Ciprofloxacin / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D004755:Enterobacteriaceae / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology; D006801:Humans; D011634:Public Health; D018570:Risk Assessment; D012475:Salmonella / Q000187:drug effects / Q000302:isolation & purification* / Q000472:pathogenicity; D014865:Waste Disposal, Fluid / Q000379:methods*; D014871:Water Microbiology
D000890:Anti-Infective Agents; D002939:Ciprofloxacin