Rapid transport of neural intermediate filament protein.
Peripherin is a neural intermediate filament protein that is expressed in peripheral and enteric neurons, as well as in PC12 cells. A determination of the motile properties of peripherin has been undertaken in PC12 cells during different stages of neurite outgrowth. The results reveal that non-filamentous, non-membrane bound peripherin particles and short peripherin intermediate filaments, termed 'squiggles', are transported at high speed throughout PC12 cell bodies, neurites and growth cones. These movements are bi-directional, and the majority require microtubules along with their associated molecular motors, conventional kinesin and cytoplasmic dynein. Our data demonstrate that peripherin particles and squiggles can move as components of a rapid transport system capable of delivering cytoskeletal subunits to the most distal regions of neurites over relatively short time periods.
Helfand
Brian T
BT
Department of Cell and Molecular Biology, Northwestern University Feinberg School of Medicine, 303 East Chicago Avenue, Ward 11-145, Chicago, IL 60611, USA.
Loomis
Patty
P
Yoon
Miri
M
Goldman
Robert D
RD
D000818:Animals; D001370:Axonal Transport / Q000502:physiology*; D000072159:Dynactin Complex; D004398:Dyneins / Q000378:metabolism; D007381:Intermediate Filament Proteins / Q000235:genetics / Q000378:metabolism*; D007382:Intermediate Filaments / Q000378:metabolism*; D016547:Kinesins / Q000378:metabolism; D008562:Membrane Glycoproteins; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D016501:Neurites / Q000378:metabolism*; D016716:PC12 Cells; D064531:Peripherins; D021381:Protein Transport / Q000502:physiology; D051381:Rats; D014162:Transfection
D000072159:Dynactin Complex; D007381:Intermediate Filament Proteins; D008562:Membrane Glycoproteins; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D064531:Peripherins; D004398:Dyneins; D016547:Kinesins