Polo-like kinase Cdc5 promotes chiasmata formation and cosegregation of sister centromeres at meiosis I.
During meiosis, two rounds of chromosome segregation occur after a single round of DNA replication, producing haploid progeny from diploid progenitors. Three innovations in chromosome behaviour during meiosis I accomplish this unique division. First, crossovers between maternal and paternal sister chromatids (detected cytologically as chiasmata) bind replicated maternal and paternal chromosomes together. Second, sister kinetochores attach to microtubules from the same pole (mono-polar orientation), causing maternal and paternal centromere pairs (and not sister chromatids) to be separated. Third, sister chromatid cohesion near centromeres is preserved at anaphase I when cohesion along chromosome arms is destroyed. The finding that destruction of mitotic cohesion is regulated by Polo-like kinases prompted us to investigate the meiotic role of the yeast Polo-like kinase Cdc5. We show here that cells lacking Cdc5 synapse homologues and initiate recombination normally, but fail to efficiently resolve recombination intermediates as crossovers. They also fail to properly localize the Lrs4 (ref. 3) and Mam1 (ref. 4) monopolin proteins, resulting in bipolar orientation of sister kinetochores. Cdc5 is thus required both for the formation of chiasmata and for cosegregation of sister centromeres at meiosis I.
Clyne
Rosemary K
RK
Research Institute of Molecular Pathology (IMP), Vienna A-1030, Austria.
Katis
Vittorio L
VL
Jessop
Lea
L
Benjamin
Kirsten R
KR
Herskowitz
Ira
I
Lichten
Michael
M
Nasmyth
Kim
K
D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins; D002478:Cells, Cultured; D002503:Centromere / Q000235:genetics*; D020090:Chromosome Segregation / Q000235:genetics*; D002875:Chromosomes / Q000235:genetics; D004261:DNA Replication / Q000235:genetics; D005057:Eukaryotic Cells / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D015966:Gene Expression Regulation, Fungal / Q000235:genetics; D018386:Kinetochores / Q000378:metabolism; D008540:Meiosis / Q000235:genetics*; D011494:Protein Kinases / Q000172:deficiency / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism; D015003:Yeasts / Q000201:enzymology* / Q000235:genetics*
D018528:ATP-Binding Cassette Transporters; D018797:Cell Cycle Proteins; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; C107004:mam1 protein, S pombe; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; C081927:CDC5 protein, S cerevisiae