Organization of the endoplasmic reticulum in dividing cells of the gymnosperms Pinus brutia and Pinus nigra, and of the pterophyte Asplenium nidus.
Endoplasmic reticulum (ER) organization in the dividing cells of the pterophyte Asplenium nidus and of the gymnosperms Pinus brutia and Pinus nigra has been studied by immunolocalization techniques using the monoclonal antibody 2E7, which recognizes luminar ER resident proteins containing C-terminal HDEL sequences. In the pterophyte, the ER reorganization during cell cycle is similar to that in angiosperms. Among others, prominent ER gatherings were found at the mitotic spindle poles and in the phragmoplast during cytokinesis. However, in the gymnosperms examined, the ER displays a unique pattern of reorganization not described so far. In both the Pinus species, well-defined ER patterns are successively formed during cell cycle. They are the preprophase ER-band, the prophase- metaphase- and anaphase ER-spindle, the interzonal ER-system, the ER-phragmoplast and an ER-system lining the daughter cell wall. The ER patterns are closely similar to that of the correspondent microtubule (MT) arrangements with which they are co-organized. Observations made on P. nigra root-cells affected by oryzalin, colchicine and cytochalasin D favour the conclusion that the pattern of ER organization is controlled during mitosis and cytokinesis by the MT cytoskeleton.
Zachariadis
M
M
Department of Botany, Faculty of Biology, National and Kapodistrian University of Athens, Athens 15784, Greece.
Quader
H
H
Galatis
B
B
Apostolakos
P
P
D008841:Actin Cytoskeleton / Q000502:physiology; D000705:Anaphase / Q000187:drug effects / Q000502:physiology; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D003078:Colchicine / Q000494:pharmacology; D015638:Cytochalasin D / Q000494:pharmacology; D004136:Dinitrobenzenes / Q000494:pharmacology; D004721:Endoplasmic Reticulum / Q000502:physiology*; D029624:Ferns / Q000166:cytology*; D006540:Herbicides / Q000494:pharmacology; D007399:Interphase / Q000187:drug effects / Q000502:physiology; D008677:Metaphase / Q000187:drug effects / Q000502:physiology; D008870:Microtubules / Q000502:physiology; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D028223:Pinus / Q000166:cytology*; D011418:Prophase / Q000187:drug effects / Q000502:physiology; D013424:Sulfanilamides; D013692:Telophase / Q000187:drug effects / Q000502:physiology
D004136:Dinitrobenzenes; D006540:Herbicides; D019384:Nucleic Acid Synthesis Inhibitors; D013424:Sulfanilamides; D015638:Cytochalasin D; C012465:oryzalin; D003078:Colchicine