Bio-artificial skin composed of gelatin and (1-->3), (1-->6)-beta-glucan.
Porous scaffolds composed of gelatin and beta-glucan were prepared using the freeze-drying method. The scaffold had an inter-connected pore structure with average pore size of 90-150 microm. Results for the contact angle and cell attachment revealed that a high gelatin content was suitable for cellular attachment and distribution in two- or three-dimensional fibroblast cultures, because the gelatin had acidic residues, and arginine-glycine-aspartic acid groups. To prepare a stratified wound dressing to mimic the normal human skin, fibroblasts and keratinocyte cells were isolated from a child's foreskin, and were co-cultured in gelatin/beta-glucan scaffolds were cross-linked using 1-ethyl-(3-3-dimethylaminopropyl) carbodiimide hydrochloride. An in vivo study showed that after 1 week, the artificial dermis containing the fibroblasts enhanced the re-epithelialization of a full-thickness skin defect rather than the acellular scaffold.
Lee
Sang Bong
SB
School of Chemical Engineering, College of Engineering, Hanyang University, Seoul 133-791, South Korea.
Jeon
Hyun Wook
HW
Lee
Young Woo
YW
Lee
Young Moo
YM
Song
Kang Won
KW
Park
Moon Hyang
MH
Nam
Young Soo
YS
Ahn
Hee Chang
HC
D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division; D002648:Child; D046508:Culture Techniques / Q000379:methods; D020405:Dermis / Q000166:cytology* / Q000503:physiopathology; D005109:Extracellular Matrix / Q000737:chemistry*; D005347:Fibroblasts / Q000637:transplantation*; D005612:Freeze Drying; D005780:Gelatin / Q000737:chemistry*; D005936:Glucans / Q000737:chemistry*; D006801:Humans; D008567:Membranes, Artificial; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D017768:Skin, Artificial; D023822:Tissue Engineering / Q000379:methods*; D016896:Treatment Outcome; D014945:Wound Healing / Q000502:physiology; D014950:Wounds, Penetrating / Q000601:surgery*
D005936:Glucans; D008567:Membranes, Artificial; C033671:epiglucan; D005780:Gelatin