Effect of basic fibroblast growth factor on vascularization in esophagus tissue engineering.
We carried out an experimental study to evaluate the effect of basic fibroblast growth factor (bFGF)-containing collagen gel on vascularization in esophageal tissue engineering. We compared an acellular collagen sponge scaffold and an acellular collagen gel scaffold in combination with bFGF using a canine model. The construct was implanted in the cervical esophagus and the regenerated tissue was evaluated one month after surgery. Histological analysis confirmed a significantly large amount of blood vessels in the bFGF-containing collagen gel group as compared to the collagen gel group without bFGF (bFGF (-)). However, in the collagen sponge groups, no difference was observed between the bFGF (+) group and the bFGF (-) group. These results showed that bFGF-containing collagen gel is suitable not only for an acellular scaffold for tissue engineering but also for an effective tropic factor vehicle in vivo.
Hori
Y
Y
Department of Bioartificial Organs, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan. hori@frontier.kyoto-u.ac.jp
Nakamura
T
T
Kimura
D
D
Kaino
K
K
Kurokawa
Y
Y
Satomi
S
S
Shimizu
Y
Y
D000818:Animals; D021522:Bioartificial Organs; D003094:Collagen / Q000494:pharmacology; D004285:Dogs; D004947:Esophagus / Q000098:blood supply* / Q000187:drug effects; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D023421:Models, Animal; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D023822:Tissue Engineering / Q000379:methods*
D016222:Fibroblast Growth Factor 2; D003094:Collagen