title: Dynamic range of fluorescence detection and base-calling accuracy in DNA sequencer based on single-photon counting.
abstract: Recently, we developed a family of high-performance automated capillary DNA sequencing instruments based on a single-photon detection of fluorescently labeled DNA fragments. Our machines employ digital and broadband techniques, essential for achieving superior instrument sensitivity and dynamic range. In the present paper, we discuss limitations of the instrument's performance caused by the nonlinearity of single-photon detectors as well as methods for nonlinearity compensation which increase the detection dynamic range and base-calling accuracy.
authors.affiliation: Department of Electrical and Computer Engineering, State University of New York, Stony Brook 11794, USA. gavrilov@ece.sunysb.edu
authors.forename: Dmitri N
authors.initials: DN
authors.lastname: Gavrilov
authors.forename: Boris
authors.initials: B
authors.lastname: Gorbovitski
authors.forename: Mikhail
authors.initials: M
authors.lastname: Gouzman
authors.forename: Georgiy
authors.initials: G
authors.lastname: Gudkov
authors.forename: Andrei
authors.initials: A
authors.lastname: Stepoukhovitch
authors.forename: Victor
authors.initials: V
authors.lastname: Ruskovoloshin
authors.forename: Andrei
authors.initials: A
authors.lastname: Tsuprik
authors.forename: Georgiy
authors.initials: G
authors.lastname: Tyshko
authors.forename: Olga
authors.initials: O
authors.lastname: Bilenko
authors.forename: Olga
authors.initials: O
authors.lastname: Kosobokova
authors.forename: Serge
authors.initials: S
authors.lastname: Luryi
authors.forename: Vera
authors.initials: V
authors.lastname: Gorfinkel
mesh_terms: D004581:Electronics; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000592:standards; D004867:Equipment Design; D005456:Fluorescent Dyes / Q000032:analysis; D009711:Nucleotides / Q000032:analysis*; D017785:Photons; D012016:Reference Values; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA / Q000295:instrumentation* / Q000592:standards
chemical_list: D005456:Fluorescent Dyes; D009711:Nucleotides