A peptide-mediated and hydroxyl radical HO*-involved oxidative degradation of cellulose by brown-rot fungi.
A special low-molecular-weight peptide named Gt factor, was isolated and purified from the extracellular culture of brown-rot fungi Gloeophyllum trabeum via gel filtration chromatography and HPLC. It has been shown to reduce Fe3+ to Fe2+. Electron paramagnetic resonance (EPR) spectroscopy revealed Gt factor was able to drive H2O2 generation via a superoxide anion O2*- intermediate and mediate the formation of hydroxyl radical HO* in the presence of O2. All the results indicated that Gt factor could oxidize the cellulose, disrupt the inter- and intrahydrogen bonds in cellulose chains by a HO*-involved mechanism. This resulted in depolymerization of the cellulose, which made it accessible for further enzymatic hydrolysis.
Wang
Wei
W
The State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, The People's Republic of China.
Gao
Peiji
P
D001487:Basidiomycota / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002482:Cellulose / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D006861:Hydrogen Peroxide / Q000378:metabolism; D017665:Hydroxyl Radical / Q000378:metabolism*; D007501:Iron / Q000378:metabolism; D018625:Microscopy, Atomic Force; D008854:Microscopy, Electron; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D010455:Peptides / Q000302:isolation & purification / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism
D010455:Peptides; D013481:Superoxides; D017665:Hydroxyl Radical; D002482:Cellulose; D006861:Hydrogen Peroxide; D007501:Iron; D010100:Oxygen