title: Levan fructotransferase from Arthrobacter oxydans J17-21 catalyzes the formation of the di-D-fructose dianhydride IV from levan.
abstract: A new levan fructotransferase (LFTase) isolated from Arthrobacter oxydans J17-21 was characterized for the production of difructose dianhydride IV (DFA IV). LFTase was purified to apparent homogeneity by Q-Sepharose ion exchange chromatography, Mono-Q HR 5/5 column chromatography, and gel permeation chromatography. The enzyme had an apparent molecular mass of 54000 Da. The optimum pH for the enzyme-catalyzed reaction was pH 6.5, and the optimum temperature was observed at 45 degrees C. The LFTase was activated by the presence of CaCl(2) and EDTA-2Na but inhibited strongly by MnCl(2) and CuSO(4) at 1 mM and completely by FeSO(4) and Ag(2)SO(4) at 1 mM. A bacterial levan from Zymomonas mobilis was incubated with an LFTase; final conversion yield from the levan to DFA IV was 35%. Neither inulin, levanbiose, sucrose, dextran, nor starch was hydrolyzed by LFTase. DFA IV was very stable at acidic pH and high temperature, thus indicating that DFA IV may be suitable for the food industry and related areas.
authors.affiliation: Graduate School of East-West Medical Science, Kyung Hee University, Suwon 449-701, Korea.
authors.forename: Ki-Hyo
authors.initials: KH
authors.lastname: Jang
authors.forename: Eun-Ja
authors.initials: EJ
authors.lastname: Ryu
authors.forename: Buem-Seek
authors.initials: BS
authors.lastname: Park
authors.forename: Ki-Bang
authors.initials: KB
authors.lastname: Song
authors.forename: Soon Ah
authors.initials: SA
authors.lastname: Kang
authors.forename: Chul Ho
authors.initials: CH
authors.lastname: Kim
authors.forename: Tai-Boong
authors.initials: TB
authors.lastname: Uhm
authors.forename: Yong-Il
authors.initials: YI
authors.lastname: Park
authors.forename: Sang-Ki
authors.initials: SK
authors.lastname: Rhee
mesh_terms: D001173:Arthrobacter / Q000201:enzymology*; D002852:Chromatography, Ion Exchange; D004187:Disaccharides / Q000737:chemistry*; D005630:Fructans / Q000378:metabolism*; D006602:Hexosyltransferases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D013696:Temperature; D017355:Zymomonas / Q000737:chemistry
chemical_list: D004187:Disaccharides; D005630:Fructans; D011135:Polysaccharides, Bacterial; C033869:difructose anhydride IV; C072599:levan; D006602:Hexosyltransferases; C040074:levan fructotransferase