title: Effect of nucleotide excision repair on ENU-induced mutation in female germ cells of Drosophila melanogaster.
abstract: The role of nucleotide excision repair (NER) in the repair of alkylation damage in the germ cells of higher eukaryotes has been studied mainly by treating postmeiotic male germ cells. Little is known about repair in actively repairing female germ cells. In this study, we treated NER-deficient (ner(-)) mus201(D1) Drosophila females with N-ethyl-N-nitrosourea (ENU) and determined both the mutant frequencies in the multiple locus recessive lethal (RL) test and in the single locus vermilion gene and determined the ENU mutation spectrum in the vermilion gene. The results show that ENU is mutagenic in all cell stages and that the induced frequencies increase with cell maturation, from oogonia to mature oocytes. In addition, the induced spectrum consists mainly of A:T-->T:A transversions (43.8%), A:T-->G:C transitions (21.9%), and A:T-->C:G transversions (15.6%). G:C-->A:T (3.1%) transitions, other transversions (9.4%), frameshifts (3.1%), and deletions (3.1%) were also found. Comparison of these results with those previously obtained for repair-proficient (ner(+)) female germ cells reveal: 1) Differences in the RL and vermilion mutation frequencies for ner(+) and ner(-) germ cells, indicating that NER is involved in the repair of ENU-induced damage to these cells. 2) At least 15.6% of mutations in ner(-) cells may be the consequence of N-ethylation damage and mutations of this type were not detected in ner(+) cells. 3) Although differences were found in transition frequencies between ENU-treated ner(+) and ner(-) germ cells (52.2% vs. 25%), suggesting that a functional NER is involved in processing O-ethylated damage, the role of NER in repairing O-ethylated adducts is uncertain.
authors.affiliation: Departamento de Biología Funcional e Instituto Universitario de Oncología del Principado de Asturias, Area de Genética, Universidad de Oviedo, Oviedo, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Alvarez
authors.forename: M A
authors.initials: MA
authors.lastname: Comendador
authors.forename: L M
authors.initials: LM
authors.lastname: Sierra
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004260:DNA Repair; D029721:Drosophila Proteins / Q000235:genetics; D004331:Drosophila melanogaster; D005038:Ethylnitrosourea / Q000633:toxicity*; D005136:Eye Proteins; D005260:Female; D005854:Germ Cells / Q000187:drug effects* / Q000648:ultrastructure; D009153:Mutagens / Q000633:toxicity*; D009154:Mutation; D014366:Tryptophan Oxygenase / Q000235:genetics
chemical_list: D017931:DNA Primers; D029721:Drosophila Proteins; D005136:Eye Proteins; D009153:Mutagens; D014366:Tryptophan Oxygenase; C121974:v protein, Drosophila; D005038:Ethylnitrosourea