title: Concentration-dependent actions of glial chondroitin sulfate on the neuritic growth of midbrain neurons.
abstract: Astrocytes located in two distinct regions of midbrain differ in their neuritic growth support abilities. Midbrain neurons cultured onto astrocyte monolayers from the lateral (L) region develop long and branched neurites while neurons cultured onto astrocyte monolayers from the medial (M) region develop short or no neurites. The extracellular matrix of these astrocytes has an important role in promoting or inhibiting the growth of these neurons. Differences on the compartmental distribution, as well as on the concentration of GAGs of L and M astrocytes, may be related to their differential capacity of supporting neuritic growth. Indeed, enzymatic digestion of heparan sulfate (HS) and chondroitin sulfate (CS) chains also pointed to an important function for GAGs on axon navigation. In order to better characterize the role of CS on the growth of midbrain neurites, we treated L and M astrocyte monolayers with 1 mM of beta-D-xyloside. Under these conditions, astrocytes oversynthesized and secreted CS protein-free chains to the culture medium. M astrocytes had a significant reduction in their neuritic growth-inhibiting ability after xyloside treatment, suggesting a promoting role for soluble CS in neuritic growth. Chondroitin 4-sulfate (CS-4) added in different concentrations to M astrocyte cultures turned this glia into a permissive substrate, acting in a linear way as far as the largest neurite was concerned. However, a U-shaped dose-effect curve on neurite growth resulted from the similar treatment of L astrocytes. These results suggest that glial CS-4 could be involved in the neurite growth modulating properties of midbrain neurons in a complex concentration-dependent way.
authors.affiliation: Departamento de Anatomia, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, 21949-590, Rio de Janeiro, Brazil.
authors.forename: Fábio A
authors.initials: FA
authors.lastname: Mendes
authors.forename: Glaucia R
authors.initials: GR
authors.lastname: Onofre
authors.forename: Luiz C F
authors.initials: LC
authors.lastname: Silva
authors.forename: Leny A
authors.initials: LA
authors.lastname: Cavalcante
authors.forename: José
authors.initials: J
authors.lastname: Garcia-Abreu
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D002450:Cell Communication / Q000187:drug effects / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000378:metabolism* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005109:Extracellular Matrix / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000378:metabolism; D006027:Glycosides / Q000494:pharmacology; D006133:Growth Substances / Q000378:metabolism*; D008636:Mesencephalon / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D051379:Mice; D016501:Neurites / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D011509:Proteoglycans / Q000378:metabolism
chemical_list: D006025:Glycosaminoglycans; D006027:Glycosides; D006133:Growth Substances; D011509:Proteoglycans; C010807:xylosides; D002809:Chondroitin Sulfates