title: Immobilisation of glucose oxidase within metallic nanotubes arrays for application to enzyme biosensors.
abstract: Arrays of nanoscopic gold tubes were prepared by electroless deposition of the metal within the pores of polycarbonate particle track-etched membranes (PTM). Glucose oxidase (Gox) was immobilised onto preformed self-assembled monolayers (SAMs) (mercaptoethylamine or mercaptopropionic acid) on electroless gold via cross-linking with glutaraldehyde or covalent attachment by carbodiimide coupling. The effectiveness of the different steps in the Gox immobilisation procedure was assessed by contact angle measurements, cyclic voltammetry and X-ray photoelectron spectroscopy. The enzyme loading was estimated by radioactivity measurements. The sensitivity to beta-glucose of these different biosensors has been evaluated. Glucose responses as large as 400 nA mM(-1) cm(-2) have been obtained. To our knowledge, this sensitivity value is amongst the highest values reported in the literature for comparable biosensor systems.
authors.affiliation: Unité de Chimie et de Physique des Hauts Polymères, Université Catholique de Louvain, Place Croix du Sud, 1, B-1348, Louvain-la-Neuve, Belgium.
authors.forename: Marc
authors.initials: M
authors.lastname: Delvaux
authors.forename: Sophie
authors.initials: S
authors.lastname: Demoustier-Champagne
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D003432:Cross-Linking Reagents; D004563:Electrochemistry; D004800:Enzymes, Immobilized; D005947:Glucose / Q000032:analysis; D005949:Glucose Oxidase; D006046:Gold; D008855:Microscopy, Electron, Scanning; D036103:Nanotechnology; D043942:Nanotubes
chemical_list: D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D006046:Gold; D005949:Glucose Oxidase; D005947:Glucose