Bimodality of cardiac vagal afferent C-fibres in the rat.
Vagal afferent C-fibres from the heart constitute an important input to the neurogenic cardiovascular regulation. These fibres respond to altered cardiac filling pressures and to chemical stimuli. In rats, we tested whether cardiac vagal afferent C-fibres react exclusively to one stimulus (chemical or mechanical) or whether the fibres are bimodal, i.e. responsive to either kind of stimulus. As a mechanical stimulus, an indwelling balloon was inflated in the aorta to increase left ventricular end-diastolic pressure. The serotonin 5HT(3) receptor agonist phenylbiguanide was injected into the pericardial sac as a chemical stimulus. An increase of fibre activity by more than two standard deviations compared with control was considered a response to a stimulus. Most fibres (42 out of 57) responded to both stimuli and were categorized as bimodal, 9 fibres were solely mechanosensitive and 6 were solely chemosensitive. Hence, the majority of cardiac vagal C-fibres are likely to be bimodal, responding to both cardiac filling pressure and serotonin 5HT(3) receptor stimulation. Our results emphasize the potential role of endogenous mediators in the afferent limb of cardiac reflexes.
Veelken
Roland
R
Nephrologisches Labor, Department of Medicine IV, University of Erlangen-Nürnberg, Loschgestrasse 8, 91054 Erlangen, Germany. mfm431@rzmail.uni-erlangen.de
Stetter
Alexander
A
Dickel
Tobias
T
Hilgers
Karl F
KF
D000818:Animals; D001011:Aorta; D002404:Catheterization; D006321:Heart / Q000294:innervation*; D008297:Male; D008465:Mechanoreceptors / Q000502:physiology; D036421:Nerve Fibers, Unmyelinated / Q000502:physiology*; D009475:Neurons, Afferent / Q000502:physiology* / Q000648:ultrastructure; D010812:Physical Stimulation; D051381:Rats; D017207:Rats, Sprague-Dawley; D044406:Receptors, Serotonin, 5-HT3 / Q000502:physiology; D012701:Serotonin / Q000502:physiology; D017366:Serotonin Receptor Agonists / Q000494:pharmacology; D013268:Stimulation, Chemical; D014630:Vagus Nerve / Q000166:cytology / Q000502:physiology*; D017725:Ventricular Pressure
D044406:Receptors, Serotonin, 5-HT3; D017366:Serotonin Receptor Agonists; D012701:Serotonin