In vitro IL-6 production by EBV-immortalized B lymphocytes from young and elderly people genotyped for -174 C/G polymorphism in IL-6 gene: a model to study the genetic basis of inflamm-aging.
In the present investigation we analysed Interleukin 6 (IL-6) in vitro production by Epstein-Barr virus (EBV)-immortalized B lymphocytes established from 43 subjects, 15 young people and 28 elderly people, including 18 centenarians, after 3, 6, 9, 24, 48 and 72 h of culture. The subjects were genotypized for the C to G transition at nucleotide -174 of IL-6 gene promoter (-174 C/G) and were classified as C allele carriers (C+) and non-carriers (C-). We found that: (i) the interindividual difference in in vitro IL-6 production was wider in elderly individuals in respect to young individuals, leading to different coefficient of variation in the two groups; (ii) the -174 C/G polymorphism had an age-related effect on IL-6 in vitro production. Only among C- people, cells from elderly subjects produced significant higher level of IL-6 than cells from young subjects. These data are consistent with our previous results regarding the IL-6 serum levels in a large group of people of different age, including centenarians. Thus, the EBV-immortalized B lymphocytes can be considered a useful in vitro model for studying the genetic control of IL-6 production and its changes with age.
Olivieri
Fabiola
F
Italian National Research Centers on Aging (I.N.R.C.A.), Via S. Margherita, n. 5, 60121, Ancona, Italy.
Bonafè
Massimiliano
M
Giovagnetti
Simona
S
Stecconi
Rosalia
R
Cardelli
Maurizio
M
Cavallone
Luca
L
Spazzafumo
Liana
L
Marchegiani
Francesca
F
Carrieri
Giuseppina
G
Mugianesi
Elena
E
Giampieri
Claudia
C
Centurelli
Matteo
M
Moresi
Raffaella
R
Tesei
Silvia
S
Lisa
Rosamaria
R
Viticchi
Claudio
C
Falsetti
Lorenzo
L
Salvioli
Stefano
S
Franceschi
Claudio
C
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000235:genetics* / Q000276:immunology*; D001402:B-Lymphocytes / Q000502:physiology*; D002461:Cell Line, Transformed; D005260:Female; D005838:Genotype; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D066298:In Vitro Techniques; D015850:Interleukin-6 / Q000235:genetics* / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic
D015850:Interleukin-6