title: Amylin in human pancreatic islets.
abstract: OBJECTIVE
  Double and triple immunocytochemical staining was used to co-localise amylin with four pancreatic hormones, including insulin, glucagon, somatostatin and pancreatic polypeptide.
  
  
  METHODS
  Normal human adult islets were initially stained for amylin using the avidin-biotin alkaline phosphatase method. The stained sections were subsequently stained for four pancreatic hormones, respectively, with the immunoperoxidase method.
  
  
  RESULTS
  The same islet cells that were positively stained for amylin, were also positively stained for insulin, and were positively stained for glucagon, somatostatin and pancreatic polypeptide in lesser cell numbers.
  
  
  CONCLUSIONS
  Amylin is known to co-secrete and is co-localised with insulin and three other islet hormones are co-localised with amylin. This co-localisation of amylin with all four pancreatic hormones may support a role for amylin in secretory processes of these hormones.
authors.affiliation: Department of Pathology, University of Kansas Medical Center, Kansas City, Kansas, USA. Ttomita@ttmcelp.ttuhsc.edu
authors.forename: T
authors.initials: T
authors.lastname: Tomita
mesh_terms: D000682:Amyloid / Q000378:metabolism*; D005934:Glucagon / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007328:Insulin / Q000378:metabolism; D058228:Islet Amyloid Polypeptide; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism*; D010191:Pancreatic Polypeptide / Q000378:metabolism; D013004:Somatostatin / Q000378:metabolism
chemical_list: D000682:Amyloid; D007328:Insulin; D058228:Islet Amyloid Polypeptide; D013004:Somatostatin; D010191:Pancreatic Polypeptide; D005934:Glucagon