Recombinant human albumin as protein source in culture media used for IVF: a prospective randomized study.
Oocytes obtained from 85 women undergoing IVF/intracytoplasmic sperm injection (ICSI) treatment were randomly selected for culture in media containing either human serum albumin (HSA), (n = 42) or recombinant human albumin (rHA), (n = 43). At the time of transfer, the two embryos with the overall best morphology were selected on day 2, 3 or 5. Comparable rates of fertilization, cleavage, blastocyst formation and implantation were observed in both groups. The frequency of pregnancy and early pregnancy loss were also equal. It is concluded that culture media containing rHA seem to produce embryos of high quality comparable to media containing HSA. Therefore, rHA may replace HSA as a protein source in culture media for IVF and may reduce risks of prion contamination and transmission of plasma derived impurities.
Bungum
M
M
Fertility Clinic, Skive Sygehus, Resenvej 25, Denmark. mona.bungum@vibamt.dk
Humaidan
P
P
Bungum
L
L
D000418:Albumins / Q000378:metabolism*; D003470:Culture Media / Q000378:metabolism; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D009865:Oocytes / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism*; D020554:Sperm Injections, Intracytoplasmic
D000418:Albumins; D003470:Culture Media; D011994:Recombinant Proteins