Effectiveness of stannous fluoride and calcium hydroxide against Enterococcus faecalis.
The objective of this study was to evaluate the antimicrobial activity of stannous fluoride, calcium hydroxide, or the two in combination against Enterococcus faecalis. Fifteen Mueller-Hinton agar plates were used to conduct a disc diffusion susceptibility test. The plates were inoculated with E. faecalis. The groups tested were: (a) stannous fluoride (Stanimax); (b) calcium hydroxide; and (c) a combination of stannous fluoride and calcium hydroxide. Three filter paper discs, each soaked with one of the groups, were placed along with an antibiotic control (ampicillin) on each agar plate. The plates were then incubated at 37 degrees C. The zones of inhibition were measured at 24 and 48 h. One-way ANOVA revealed a statistically significant difference (p < 0.05) between the three groups. Stannous fluoride displayed the widest zone (1.7 mm), followed by the combination of stannous fluoride and calcium hydroxide (1.1 mm), whereas calcium hydroxide by itself displayed a minimal zone of inhibition (0.05 mm).
Mickel
André K
AK
Postgraduate Endodontics Program, Department of Endodontics, Case Western Reserve University School of Dentistry, Cleveland, OH 44106-4905, USA.
Sharma
Priya
P
Chogle
Sami
S
D000704:Analysis of Variance; D000891:Anti-Infective Agents, Local / Q000494:pharmacology; D002126:Calcium Hydroxide / Q000494:pharmacology*; D004338:Drug Combinations; D013293:Enterococcus faecalis / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D012388:Root Canal Irrigants / Q000494:pharmacology*; D014002:Tin Fluorides / Q000494:pharmacology*
D000891:Anti-Infective Agents, Local; D004338:Drug Combinations; D012387:Root Canal Filling Materials; D012388:Root Canal Irrigants; D014002:Tin Fluorides; D002126:Calcium Hydroxide