title: Peptide-binding assessment using mass spectrometry as a new screening method for skin sensitization.
abstract: Skin sensitization potential of low molecular weight chemicals was assessed by analyzing peptide-conjugate formation. Chemicals were incubated with a peptide, glutathione, and resultant mixtures were analyzed by mass spectrometry. Eighteen chemicals were assessed, and new peaks corresponding to chemical-peptide conjugates were detected for 13 of 14 known sensitizers. Conjugates were not detected for 4 negative chemicals. The method has advantages as a simple screening assay for assessing the sensitization potential of chemicals.
authors.affiliation: Environmental Health Science Laboratory, Sumitomo Chemical Co., Ltd., 3-1-98 Kasugadenaka, Konohana-ku, Osaka 554-8558, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kato
authors.forename: Masahiko
authors.initials: M
authors.lastname: Okamoto
authors.forename: Kazuko
authors.initials: K
authors.lastname: Yamashita
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Fukumori
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Nakai
authors.forename: Hideo
authors.initials: H
authors.lastname: Kaneko
mesh_terms: D000818:Animals; D017449:Dermatitis, Allergic Contact / Q000175:diagnosis*; D004137:Dinitrochlorobenzene / Q000032:analysis / Q000737:chemistry; D005978:Glutathione / Q000032:analysis / Q000737:chemistry; D006168:Guinea Pigs; D013058:Mass Spectrometry; D010455:Peptides / Q000032:analysis / Q000737:chemistry; D011485:Protein Binding / Q000187:drug effects*; D023422:Skin Irritancy Tests / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014118:Toxins, Biological / Q000009:adverse effects / Q000032:analysis / Q000737:chemistry; D014302:Trinitrobenzenesulfonic Acid / Q000032:analysis / Q000737:chemistry
chemical_list: D004137:Dinitrochlorobenzene; D010455:Peptides; D014118:Toxins, Biological; D014302:Trinitrobenzenesulfonic Acid; D005978:Glutathione