Determination of serum astragaloside in rats fed with Buyanghuanwu dicoction using solidphase extraction, chemical derivatization and fluorospectrophotometry.
OBJECTIVE
To develop a method for determining the serum astragaloside in rats fed with Buyanghuanwu dicoction (a preparation of a traditional Chinese medicine).


METHODS
A solid-phase extraction column was employed for pretreating the serum samples. The fluorescence characteristics of the extract were measured following chemical derivatization with anisic acid-sulphuric acid.


RESULTS
The endogenous interference was removed by solid-phase extraction. The excitation and emission maxima of the reaction product from astragaloside and anisic acid were at 310 nm and 376 nm respectively. The linear range of the calibration curve was 1.0 to 10.0 microg/ml with r=0.999 9. The average recovery rate of the method was 97% with relative standard derivation of 2.59%(n=5).


CONCLUSIONS
This method is rapid and highly sensitive, and useful for monitoring the concentration of astragaloside in rat serum.
You
Wen-wei
WW
Teaching and Research Section of Chemistry, Department of Basic Medical Sciences, First Military Medical University, Guangzhou 510515, China.
Wu
Zhao-hui
ZH
Tong
Li
L
Xing
Xue-feng
XF
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D051381:Rats; D012503:Saponins / Q000097:blood*; D013053:Spectrophotometry; D014315:Triterpenes / Q000097:blood*
D004365:Drugs, Chinese Herbal; D012503:Saponins; D014315:Triterpenes; C460102:buyang huanwu; C052064:astragaloside A