title: Production of laccase and manganese peroxidase by Fomes sclerodermeus grown on wheat bran.
abstract: The aim of this work was to study the growth and production of ligninolytic enzymes by Fomes sclerodermeus using a natural medium based on wheat bran as the principal substrate in a solid-state fermentation. Growth was monitored by measuring the chitin content in the substrate. The maximum rate of growth was observed between days 7 and 18. A 38% total dry-weight loss of the substrate was measured after 28 days of cultivation. Differential hydrolysis of the substrate revealed that cellulose was more extensively degraded than lignin. In the 28-day incubation period, the losses of cellulose and lignin were 38 and 15%, respectively. No lignin peroxidase activity was found in any of the media tested. The maximum manganese-dependent peroxidase activity recorded was 6.3 U g(-1) at 14 days, while the maximum laccase activity was 270 U g(-1) at 28 days post-inoculation. Addition of commonly used inducers such as copper or manganese did not produce a further increase in the enzyme activities, nor did addition of glucose, asparagine, or malt extract.
authors.affiliation: Laboratorio de Micología Experimental, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria Pab.II, 1428, Buenos Aires, Argentina.
authors.forename: V L
authors.initials: VL
authors.lastname: Papinutti
authors.forename: L A
authors.initials: LA
authors.lastname: Diorio
authors.forename: F
authors.initials: F
authors.lastname: Forchiassin
mesh_terms: D002482:Cellulose / Q000378:metabolism; D002686:Chitin / Q000032:analysis; D003470:Culture Media / Q000378:metabolism; D004043:Dietary Fiber / Q000378:metabolism / Q000382:microbiology*; D005285:Fermentation; D005658:Fungi / Q000201:enzymology* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D042845:Laccase; D008031:Lignin / Q000378:metabolism; D010088:Oxidoreductases / Q000096:biosynthesis*; D010544:Peroxidases / Q000096:biosynthesis* / Q000378:metabolism; D012995:Solubility
chemical_list: D003470:Culture Media; D004043:Dietary Fiber; D002686:Chitin; D002482:Cellulose; D008031:Lignin; D010088:Oxidoreductases; D042845:Laccase; D010544:Peroxidases; C042858:lignin peroxidase; C051129:manganese peroxidase