title: Genetic background affects relative nonsense mRNA accumulation in wild-type and upf mutant yeast strains.
abstract: The Saccharomyces cerevisiae nonsense-mediated mRNA decay (NMD) pathway targets mRNAs with premature stop codons and some wild-type mRNAs for accelerated decay. Upf1p, Upf2p and Upf3p are required for NMD. NMD-targeted mRNAs are degraded rapidly in wild-type cells and stabilized in upf1, upf2 or upf3 mutants. We report here that the relative CYH2 pre-mRNA/mRNA accumulation is enhanced in cells derived from a W303 background, compared with a variety of commonly used strains. The enhanced CYH2 pre-mRNA accumulation phenotype results from a larger difference in mRNA half-lives in the W303 strains than two previously used strains. This phenotype can be selected in crosses and is also seen in upf2 and upf3 mutants. These results suggest there are genes that influence the efficiency of NMD and that yeast strains derived from the W303 background may be useful for measurement of abundance and half-lives of low abundance, short-lived NMD substrates.
authors.affiliation: School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE 68588-0666, USA.
authors.forename: Bessie
authors.initials: B
authors.lastname: Kebaara
authors.forename: Tara
authors.initials: T
authors.lastname: Nazarenus
authors.forename: Rachel
authors.initials: R
authors.lastname: Taylor
authors.forename: Audrey L
authors.initials: AL
authors.lastname: Atkin
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D015152:Blotting, Northern; D018389:Codon, Nonsense / Q000235:genetics*; D003433:Crosses, Genetic; D020365:RNA Helicases / Q000378:metabolism; D020871:RNA Stability / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D015534:Trans-Activators / Q000378:metabolism; D014170:Transformation, Genetic
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D018389:Codon, Nonsense; C092470:NMD2 protein, S cerevisiae; D016601:RNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; C096360:UPF3 protein, S cerevisiae; C074151:NAM7 protein, S cerevisiae; D020365:RNA Helicases