[Nitrate assimilation activity of yellow lupine root nodules ?].
Low nitrate assimilation activity of the root nodules was demonstrated by assaying the nitrate reductase, glutamate synthase, glutamate dehydrogenase, and asparagine synthase activities, as well as the kinetics of 14C-labeled saccharose incorporation in the amino acids and amides of the cortex and the bacteroid-containing root nodule zones. Irrespective of the exogenous nitrogen concentration (0, 11.2, or 25 mM NO3-), nitrate concentration in the nodules was low as compared to the plant roots, leaves, and stems. This allowed us to propose the presence of structural and/or metabolic barriers in the nodules limiting nitrate accessibility and assimilation.
Izmaĭlov
S F
SF
Timiryazev Institute of Plant Physiology, Russian Academy of Science, Botanicheskaya ul. 35, Moscow, 127176 Russia.
Bruskova
R K
RK
Chechetka
S A
SA
Kirnos
S V
SV
Nikiforova
T A
TA
Satskaya
M V
MV
D000596:Amino Acids / Q000096:biosynthesis; D002250:Carbon Radioisotopes / Q000378:metabolism; D031297:Lupinus / Q000378:metabolism*; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism*; D018547:Plant Stems / Q000378:metabolism; D035281:Plant Tubers / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism
D000596:Amino Acids; D002250:Carbon Radioisotopes; D009566:Nitrates; D000644:Quaternary Ammonium Compounds; D013395:Sucrose; D009565:Nitrate Reductases; D050901:Nitrate Reductase; D009584:Nitrogen
Nitratassimiliruiushchaia funktsiia kluben'kov liupina zheltogo ?.