title: Cation channels trigger apoptotic death of erythrocytes.
abstract: Erythrocytes are devoid of mitochondria and nuclei and were considered unable to undergo apoptosis. As shown recently, however, the Ca(2+)-ionophore ionomycin triggers breakdown of phosphatidylserine asymmetry (leading to annexin binding), membrane blebbing and shrinkage of erythrocytes, features typical for apoptosis in nucleated cells. In the present study, the effects of osmotic shrinkage and oxidative stress, well-known triggers of apoptosis in nucleated cells, were studied. Exposure to 850 mOsm for 24 h, to tert-butyl-hydroperoxide (1 mM) for 15 min, or to glucose-free medium for 48 h, all elicit erythrocyte shrinkage and annexin binding, both sequelae being blunted by removal of extracellular Ca(2+) and mimicked by ionomycin (1 microM). Osmotic shrinkage and oxidative stress activate Ca(2+)-permeable cation channels and increase cytosolic Ca(2+) concentration. The channels are inhibited by amiloride (1 mM), which further blunts annexin binding following osmotic shock, oxidative stress and glucose depletion. In conclusion, osmotic and oxidative stress open Ca(2+)-permeable cation channels in erythrocytes, thus increasing cytosolic Ca(2+) activity and triggering erythrocyte apoptosis.
authors.affiliation: Department of Physiology, University of Tübingen, Tübingen, Germany.
authors.forename: K S
authors.initials: KS
authors.lastname: Lang
authors.forename: C
authors.initials: C
authors.lastname: Duranton
authors.forename: H
authors.initials: H
authors.lastname: Poehlmann
authors.forename: S
authors.initials: S
authors.lastname: Myssina
authors.forename: C
authors.initials: C
authors.lastname: Bauer
authors.forename: F
authors.initials: F
authors.lastname: Lang
authors.forename: T
authors.initials: T
authors.lastname: Wieder
authors.forename: S M
authors.initials: SM
authors.lastname: Huber
mesh_terms: D000584:Amiloride / Q000494:pharmacology; D017302:Annexins / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D002118:Calcium / Q000493:pharmacokinetics; D002412:Cations / Q000378:metabolism*; D002452:Cell Count; D048429:Cell Size / Q000187:drug effects; D003600:Cytosol / Q000737:chemistry / Q000187:drug effects; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007473:Ion Channels / Q000037:antagonists & inhibitors / Q000502:physiology*; D015759:Ionomycin / Q000494:pharmacology; D007476:Ionophores / Q000494:pharmacology; D009997:Osmotic Pressure / Q000187:drug effects; D018384:Oxidative Stress / Q000502:physiology*; D018408:Patch-Clamp Techniques; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
chemical_list: D017302:Annexins; D002412:Cations; D007473:Ion Channels; D007476:Ionophores; D015759:Ionomycin; D000584:Amiloride; D020122:tert-Butylhydroperoxide; D005947:Glucose; D002118:Calcium