High level of expression of recombinant human granulocyte-macrophage colony stimulating factor in transgenic rice cell suspension culture.
Recombinant human granulocyte-macrophage colony stimulating factor (hGM-CSF) has been previously produced in tobacco cell suspension cultures. However, the amount of hGM-CSF accumulated in the culture medium dropped quickly from its maximum of 150 microg/L at 5 d after incubation. To overcome this problem, we sought an expression system in which heterologous gene expression could be induced at high levels. We selected a rice amylase expression system in which the promoter Ramy3D is induced to express recombinant protein by sucrose starvation. This induction system was found to give good yield of recombinant hGM-CSF in transgenic rice cell suspension culture and protease activity of this culture medium was low compared to that of tobacco culture system.
Shin
Yun-Ji
YJ
Division of Biological Sciences and the Research Center for Bioactive Materials, Chonbuk National University, Jeonju 561-756, South Korea.
Hong
Shin-Young
SY
Kwon
Tae-Ho
TH
Jang
Yong-Suk
YS
Yang
Moon-Sik
MS
D002478:Cells, Cultured; D010450:Endopeptidases / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis* / Q000235:genetics*; D006801:Humans; D012275:Oryza / Q000235:genetics* / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods*; D011786:Quality Control; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism
D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D010450:Endopeptidases