Evaluation and application of conducting polymer entrapment on quartz crystal microbalance in flow injection immunoassay.
An immunosensor employing conducting polymer entrapment (CPE) method to immobilize immuno-protein on the quartz crystal microbalance (QCM) for clinical flow-injection-analysis (FIA) purpose was exploited. By comparing the CPE approach and the conventional physical immobilization (PI) method, a frequency shift of the former was 47% higher than that of the latter when measuring at 0.5 mg/ml human serum albumin antibody concentration. This implied that CPE was a feasible approach for developing QCM-FIA process. An immunoassay of anti-pseudorabies virus antibody in mouse sera further exemplified its practical potential in diagnostic implication.
Liu
Yung-Chuan
YC
Department of Chemical Engineering, National Chung Hsing University, 402, Taichung, Taiwan, ROC. ycliu@dragon.nchu.edu.tw
Wang
Chih-Ming
CM
Hsiung
Kuang-Pin
KP
Huang
Chienjin
C
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D015374:Biosensing Techniques / Q000295:instrumentation*; D017022:Flow Injection Analysis / Q000295:instrumentation; D011558:Herpesvirus 1, Suid / Q000276:immunology; D007118:Immunoassay / Q000295:instrumentation; D051379:Mice; D011108:Polymers; D011791:Quartz
D000914:Antibodies, Viral; D011108:Polymers; D011791:Quartz