title: Study on the sorption properties of alpha1-acid glycoprotein (AGP)-based stationary phase modified by organic solvents.
abstract: An alpha(1)-acid glycoprotein, immobilized on silica (Chiral-AGP) is one of the most widely used chiral stationary phases for the enantiomeric separation of a wide variety of chiral drugs with several applications in the biological and clinical field. The aim of this work was to study the sorption properties of the AGP-based stationary phase, which may have crucial importance for enantioselectivity. New binding data to the mechanism of the chromatographic separation are presented. The sorption of both organic solvents, i.e., acetonitrile and dioxane, shows remarkable pH dependency. A fluorescence quenching study was carried out to elucidate structural changes of AGP in the presence of acetonitrile using 2,2,2-trichloroethanol as fluorescence quencher.
authors.affiliation: Department of Pharmaceutical Chemistry, Semmelweis University, Budapest, Hungary. forkri@hogyes.sote.hu
authors.forename: Krisztina
authors.initials: K
authors.lastname: Gyimesi-Forrás
authors.forename: György
authors.initials: G
authors.lastname: Szász
authors.forename: Gábor
authors.initials: G
authors.lastname: Báthory
authors.forename: György
authors.initials: G
authors.lastname: Mészáros
authors.forename: András
authors.initials: A
authors.lastname: Gergely
mesh_terms: D000042:Absorption; D000465:Algorithms; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D005023:Ethylene Chlorohydrin / Q000031:analogs & derivatives* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D009961:Orosomucoid / Q000737:chemistry*; D012997:Solvents; D013050:Spectrometry, Fluorescence
chemical_list: D007202:Indicators and Reagents; D009961:Orosomucoid; D012997:Solvents; D005023:Ethylene Chlorohydrin; C005849:2,2,2-trichloroethanol