title: Phylogenetic analysis reveals five independent transfers of the chloroplast gene rbcL to the mitochondrial genome in angiosperms.
abstract: We used the chloroplast gene rbcL as a model to study the frequency and relative timing of transfer of chloroplast sequences to the mitochondrial genome. Southern blot survey of 20 mitochondrial DNAs confirmed three previously reported groups of plants containing rbcL in their mitochondrion, while PCR studies identified a new mitochondrial rbcL. Published and newly determined mitochondrial and chloroplast rbcL sequences were used to reconstruct rbcL phylogeny. The results imply five or six separate interorganellar transfers of rbcL among the angiosperms examined, and hundreds of successful transfers across all flowering plants. By taxonomic criteria, the crucifer transfer is the most ancient, two separate transfers within the grass family are of intermediate ancestry, and the morning-glory transfer is most recent. All five mitochondrial copies of rbcL examined exhibit insertion and/or deletion events that disrupt the reading frame (three are grossly truncated); and all are elevated in the proportion of nonsynonymous substitutions, providing clear evidence that these sequences are pseudogenes.
authors.affiliation: The Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543-1015, USA. mike@mbl.edu
authors.forename: Michael P
authors.initials: MP
authors.lastname: Cummings
authors.forename: Jacqueline M
authors.initials: JM
authors.lastname: Nugent
authors.forename: Richard G
authors.initials: RG
authors.lastname: Olmstead
authors.forename: Jeffrey D
authors.initials: JD
authors.lastname: Palmer
mesh_terms: D001483:Base Sequence; D015139:Blotting, Southern; D018742:DNA, Chloroplast / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D019143:Evolution, Molecular; D016013:Likelihood Functions; D019684:Magnoliopsida / Q000235:genetics*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010802:Phylogeny; D011544:Pseudogenes / Q000235:genetics; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D018742:DNA, Chloroplast; D004272:DNA, Mitochondrial; C101625:RbcL protein, plastid; D012273:Ribulose-Bisphosphate Carboxylase