Lack of quinine-evoked activity in rat trigeminal subnucleus caudalis.
Conflicting reports exist regarding the ability of quinine to activate neurons in the trigeminal system. We used the complementary approaches of single-unit electrophysiology and c-fos immunohistochemistry to investigate whether quinine (100 mM) activates chemonociceptive cells in the brainstem trigeminal subnucleus caudalis (Vc). In electrophysiological experiments, 38 units responded to noxious mechanical, thermal and chemical (200 mM pentanoic acid) stimuli applied to the tongue with an increase in firing rate; none responded to lingual quinine whether the quinine was presented before or after application of pentanoic acid. In the c-fos immunohistochemical experiment, both quinine and water elicited equivalent levels of fos-like immunoreactivity (FLI) in dorsomedial Vc that were significantly lower than the level of FLI evoked by pentanoic acid. These data collectively indicate that quinine does not elicit activity in chemonociceptive Vc neurons.
Simons
Christopher T
CT
Section of Neurobiology, Physiology and Behavior, University of California, Davis, 1 Shields Ave., Davis, CA 95616, USA. ctsimons@ucdavis.edu
Boucher
Yves
Y
Carstens
Mirela Iodi
MI
Carstens
E
E
D000818:Animals; D004566:Electrodes; D004594:Electrophysiology; D005073:Evoked Potentials, Somatosensory / Q000502:physiology*; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D010812:Physical Stimulation; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis; D011803:Quinine / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013268:Stimulation, Chemical; D013649:Taste / Q000502:physiology*; D014059:Tongue / Q000502:physiology; D014275:Trigeminal Caudal Nucleus / Q000378:metabolism / Q000502:physiology*
D016760:Proto-Oncogene Proteins c-fos; D011803:Quinine