A conductometric biosensor for biosecurity.
The paper describes the development of a conductometric biosensor for detecting foodborne pathogens. The biosensor consists of two components: an immunosensor that is based on electrochemical sandwich immunoassay, and a reader for signal measurement. The architecture of the immunosensor utilizes a lateral flow system that allows the liquid sample to move from one pad to another. The biosensor provides a specific, sensitive, low volume, and near real-time detection mechanism. Results are presented to highlight the performance of the biosensor for enterohemorrhagic Escherichia coli O157:H7 and Salmonella spp., which are of concern to biosecurity. The lower limit of detection is approximately 7.9 x 10(1) colony forming units per milliliter within a 10-min process. The ability to change the specificity of the antibodies will enable the biosensor to be used as a detection device for other types of foodborne pathogens.
Muhammad-Tahir
Zarini
Z
Biosystems Engineering, Michigan State University, East Lansing, MI 48824, USA.
Alocilja
Evangelyn C
EC
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D015169:Colony Count, Microbial / Q000295:instrumentation / Q000379:methods; D004553:Electric Conductivity; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D004926:Escherichia coli / Q000145:classification / Q000302:isolation & purification*; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods; D005504:Food Analysis / Q000295:instrumentation* / Q000379:methods; D005506:Food Contamination / Q000032:analysis*; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D008567:Membranes, Artificial; D019779:Salmonella enterica / Q000145:classification / Q000302:isolation & purification*; D012637:Security Measures; D013045:Species Specificity
D008567:Membranes, Artificial