title: Murine EMT-6 carcinoma: high therapeutic efficacy of microbeam radiation therapy.
abstract: Microbeam radiation therapy is an experimental modality using parallel arrays of thin (<100 micro m) slices of synchrotron-generated X rays (microplanar beams, microbeams). We used EMT-6 murine mammary carcinoma subcutaneously inoculated in the hind legs of mice to compare the therapeutic efficacies of single-fraction, unidirectional (1) "co-planar" microbeams (an array of vertically oriented microplanar beams), (2) "cross-planar" microbeams (two arrays of parallel microbeams propagated in the same direction, one with vertically and the other with horizontally oriented microplanar beams), and (3) seamless (broad) beams from the same synchrotron source. The microbeams were 90 micro m wide and were spaced 300 micro m on center; the median energy in all beams was 100 or 118 keV. Tumor ablation rates were 4/8, 4/8 and 6/7 for a 410-, 520- and 650-Gy in-slice cross-planar microbeam dose, respectively, and 1/8, 3/8, 3/7 and 6/8 for a 23-, 30-, 38- and 45-Gy broad-beam dose, respectively. When the data were pooled from the three highest doses (same average tumor ablations of 50-60%), the incidences of normal-tissue acute toxicity (moist desquamation and epilation) and delayed toxicity (failure of hair regrowth) were significantly lower for cross-planar microbeams than broad beams (P < 0.025). Furthermore, for the highest doses in these two groups, which also had the same tumor ablation rate (>75%), not only were the above toxicities lower for the cross-planar microbeams than for the broad beams (P < 0.02), but severe leg dysfunction was also lower (P < 0.003). These findings suggest that single-fraction microbeams can ablate tumors at high rates with relatively little normal-tissue toxicity.
authors.affiliation: Medical Department, Brookhaven National Laboratory, Upton, New York 11973, USA. Dilmanian@bnl.gov
authors.forename: F Avraham
authors.initials: FA
authors.lastname: Dilmanian
authors.forename: Gerard M
authors.initials: GM
authors.lastname: Morris
authors.forename: Nan
authors.initials: N
authors.lastname: Zhong
authors.forename: Tigran
authors.initials: T
authors.lastname: Bacarian
authors.forename: James F
authors.initials: JF
authors.lastname: Hainfeld
authors.forename: John
authors.initials: J
authors.lastname: Kalef-Ezra
authors.forename: Laura J
authors.initials: LJ
authors.lastname: Brewington
authors.forename: Jennifer
authors.initials: J
authors.lastname: Tammam
authors.forename: Eliot M
authors.initials: EM
authors.lastname: Rosen
mesh_terms: D000818:Animals; D005260:Female; D008325:Mammary Neoplasms, Experimental / Q000532:radiotherapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D011836:Radiation Tolerance; D011879:Radiotherapy Dosage; D017356:Synchrotrons; D014964:X-Ray Therapy / Q000009:adverse effects / Q000379:methods*