Entamoeba histolytica: rapid identification and differentiation of Indian isolates by riboprinting.
Entamoeba histolytica infection still remains one of the major public health problem for developing countries like India. A rapid and accurate detection of this parasite is essential for prevention and control of amoebiasis. In this study, using the method of 'riboprinting' (PCR-RFLP of rRNA genes from amoeba) we have analysed 15 stool samples from symptomatic patients of amoebiasis. All 15 patients of clinical amoebiasis had E. histolytica in their stool and two of the samples also showed mixed infection of E. dispar. Apart from the known restriction enzyme sites within the amoeba SSU-rRNA genes, a new Sau3A site having a discriminatory value is identified in these E. histolytica isolates from India. Hence, it is possible to rapidly identify E. histolytica DNA and differentiating it from E. dispar using minute amounts of clinical stool samples, thus eliminating the laborious parasite culturing process. Thus, riboprinting is advantageous for clearcut identification of E. histolytica in order to decide an effective antiamoebic therapy.
Mukhopadhyay
Alka
A
Department of Experimental Medicine and Biotechnology, Post Graduate Institute of Medical Education and Research, 160012, Chandigarh, India.
Chakraborti
Anuradha
A
Mahajan
Ramesh Chander
RC
Ganguly
Nirmal Kumar
NK
D000818:Animals; D016054:DNA, Protozoan / Q000032:analysis / Q000737:chemistry; D004275:DNA, Ribosomal / Q000032:analysis / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D004748:Entamoeba histolytica / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004749:Entamoebiasis / Q000175:diagnosis / Q000469:parasitology* / Q000517:prevention & control; D005243:Feces / Q000469:parasitology*; D006801:Humans; D007194:India; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D016053:RNA, Protozoan / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D015183:Restriction Mapping; D021521:Ribotyping
D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D016053:RNA, Protozoan; D012335:RNA, Ribosomal