title: Xenopus Cyr61 regulates gastrulation movements and modulates Wnt signalling.
abstract: Cyr61 is a secreted, heparin-binding, extracellular matrix-associated protein whose activities include the promotion of adhesion and chemotaxis, and the stimulation of fibroblast and endothelial cell growth. Many, if not all, of these activities of Cyr61 are mediated through interactions with integrins. We explore the role of Cyr61 in the early development of Xenopus laevis. Gain- and loss-of-function experiments show that Xcyr61 is required for normal gastrulation movements. This role is mediated in part through the adhesive properties of Xcyr61 and its related ability to modulate assembly of the extracellular matrix. In addition, Xcyr61 can, in a context-dependent manner, stimulate or inhibit signalling through the Wnt pathway. These properties of Xcyr61 provide a mechanism for integrating cell signalling, cell adhesion and cell migration during gastrulation.
authors.affiliation: Division of Developmental Biology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK.
authors.forename: B V
authors.initials: BV
authors.lastname: Latinkic
authors.forename: S
authors.initials: S
authors.lastname: Mercurio
authors.forename: B
authors.initials: B
authors.lastname: Bennett
authors.forename: E M A
authors.initials: EM
authors.lastname: Hirst
authors.forename: Q
authors.initials: Q
authors.lastname: Xu
authors.forename: L F
authors.initials: LF
authors.lastname: Lau
authors.forename: T J
authors.initials: TJ
authors.lastname: Mohun
authors.forename: J C
authors.initials: JC
authors.lastname: Smith
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019485:Bone Morphogenetic Proteins / Q000378:metabolism; D002448:Cell Adhesion; D002465:Cell Movement; D055515:Cysteine-Rich Protein 61; D003598:Cytoskeletal Proteins / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D005775:Gastrula / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D019812:Heparan Sulfate Proteoglycans / Q000378:metabolism; D017874:Immediate-Early Proteins / Q000235:genetics* / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D020319:Oligodeoxyribonucleotides, Antisense / Q000235:genetics / Q000494:pharmacology; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D015534:Trans-Activators / Q000378:metabolism; D051153:Wnt Proteins; D029867:Xenopus Proteins; D014982:Xenopus laevis / Q000196:embryology* / Q000235:genetics* / Q000378:metabolism; D029961:Zebrafish Proteins; D051176:beta Catenin
chemical_list: D019485:Bone Morphogenetic Proteins; C501850:CCN1 protein, Xenopus; C495266:CTNNB1 protein, Xenopus; D055515:Cysteine-Rich Protein 61; D003598:Cytoskeletal Proteins; D018076:DNA, Complementary; D019812:Heparan Sulfate Proteoglycans; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D020319:Oligodeoxyribonucleotides, Antisense; D011518:Proto-Oncogene Proteins; D015534:Trans-Activators; D051153:Wnt Proteins; D029867:Xenopus Proteins; D029961:Zebrafish Proteins; D051176:beta Catenin