Detection of rhinovirus RNA in middle turbinate of patients with common colds by in situ hybridization.
Human rhinovirus 14 RNA was determined by in situ hybridization from middle turbinate biopsies in 32 patients with diagnosed common colds and in five control individuals. Twenty-two (69%) biopsies from common colds patients but none of the five control biopsies showed reactivity for human rhinovirus 14 antisense probe. The signal was detected both in the respiratory epithelium and in mucosal inflammatory cells. In situ hybridization of the middle turbinate tissue yielded more positive results than RT-PCR (47%) or virus culture (34%) assayed from nasopharyngeal aspirates, but no statistical significant differences were observed (P = 0.265, P = 0.425, respectively). The results indicated that in situ hybridization procedure was slightly more sensitive than PCR assays and classical culture for the detection of human rhinovirus infection of upper respiratory tract. However, in situ hybridization procedure appeared to be an interesting methodology to investigate the physiopathology of respiratory tract infection by rhinoviruses.
Pitkäranta
Anne
A
Department of Otorhinolaryngology, University Hospital of Helsinki, Helsinki, Finland. anne.pitkaranta@hus.fi
Puhakka
Tuomo
T
Mäkelä
Mika J
MJ
Ruuskanen
Olli
O
Carpen
Olli
O
Vaheri
Antti
A
D000328:Adult; D003139:Common Cold / Q000821:virology*; D005260:Female; D006367:HeLa Cells; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D010850:Picornaviridae Infections / Q000821:virology*; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012229:Rhinovirus / Q000235:genetics / Q000302:isolation & purification*; D014420:Turbinates / Q000821:virology*; D014776:Virus Cultivation
D012367:RNA, Viral