High-performance liquid chromatographic method for the determination and pharmacokinetic study of dehydrotumulosic acid in the plasma of rats having taken the traditional chinese medicinal preparation Ling-Gui-Zhu-Gan decoction.
A high-performance liquid chromatographic method for the determination of dehydrotumulosic acid in plasma of rats having been administrated orally with the traditional Chinese medicinal preparation Ling-Gui-Zhu-Gan decoction was developed. Plasma samples taken from rats were acidified with hydrochloric acid and extracted with ethyl acetate. Separation of the main effective constituent dehydrotumulosic acid was accomplished on a C(18) stationary phase and a mobile phase of methanol-acetonitrile-2% glacial acetic acid (13:12:10, v/v), with a UV detector setting at 242 nm. After validation, the method was used for preliminary investigation of the pharmacokinetic profiles of dehydrotumulosic acid administrated in Ling-Gui-Zhu-Gan decoction.
Song
Zonghua
Z
Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, PR China.
Bi
Kaishun
K
Luo
Xu
X
D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry*; D051381:Rats; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D014315:Triterpenes / Q000097:blood* / Q000493:pharmacokinetics
D010936:Plant Extracts; D014315:Triterpenes; C457640:ling-gui-zhu-gan decoction; C102488:dehydrotumulosic acid