title: fhuA of Actinobacillus pleuropneumoniae encodes a ferrichrome receptor but is not regulated by iron.
abstract: The swine pathogen Actinobacillus pleuropneumoniae possesses a 75-kDa outer membrane protein (OMP), FhuA, the receptor for ferrichrome, a hydroxamate-type siderophore. Polyclonal serum to FhuA reacted with OMP preparations from 12 serotypes of A. pleuropneumoniae under conditions of iron repletion and restriction. Reverse transcription-PCR confirmed that A. pleuropneumoniae fhuA expression is not upregulated in response to low iron levels. An A. pleuropneumoniae fhuA deletion mutant was generated and showed abolishment of ferrichrome uptake.
authors.affiliation: Groupe de Recherche sur les Maladies Infectieuses du Porc, Département de pathologie et microbiologie, Faculté de médecine vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec, Canada J2S 7C6.
authors.forename: Leonie G
authors.initials: LG
authors.lastname: Mikael
authors.forename: Ramakrishnan
authors.initials: R
authors.lastname: Srikumar
authors.forename: James W
authors.initials: JW
authors.lastname: Coulton
authors.forename: Mario
authors.initials: M
authors.lastname: Jacques
mesh_terms: D016977:Actinobacillus pleuropneumoniae / Q000737:chemistry / Q000235:genetics*; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D005291:Ferrichrome / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D007501:Iron / Q000494:pharmacology*; D008297:Male; D008970:Molecular Weight; D011817:Rabbits; D011991:Receptors, Virus / Q000737:chemistry / Q000235:genetics* / Q000502:physiology
chemical_list: D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; C050900:FhuA protein, E coli; D011991:Receptors, Virus; D005291:Ferrichrome; D007501:Iron