title: The Candida albicans UBI3 gene encoding a hybrid ubiquitin fusion protein involved in ribosome biogenesis is essential for growth.
abstract: We have constructed a conditional null mutant Candida albicans strain for the UBI3 gene which encodes a ubiquitin fusion protein involved in ribosome biogenesis. A one-step gene disruption procedure, using the plasmid pCaDis, was designed to place the second copy of the UBI3 gene under the control of the tightly regulated MET3 promoter in a C. albicans heterozygous strain (UBI3/Deltaubi3::hisG), previously isolated in the first step of the ura-blaster protocol. Analysis of the conditional null mutant in repressing and inducing conditions indicates that UBI3 is an essential gene whose expression is required for growth of C. albicans.
authors.affiliation: Departament de Microbiologia i Ecologia, Facultat de Farmàcia, Universitat de València, Avgda. Vicent Andrés Estellés s/n, 46100 Burjassot, Spain.
authors.forename: Patricia
authors.initials: P
authors.lastname: Roig
authors.forename: Daniel
authors.initials: D
authors.lastname: Gozalbo
mesh_terms: D001483:Base Sequence; D002176:Candida albicans / Q000235:genetics* / Q000254:growth & development*; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics / Q000502:physiology*; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012269:Ribosomal Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D012270:Ribosomes / Q000502:physiology*; D014452:Ubiquitins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D012269:Ribosomal Proteins; D014452:Ubiquitins; C085995:ubi3 protein, Solanum tuberosum