Use of fluorescent in situ hybridization to evidence the presence of Helicobacter pylori in water.
We have evaluated the use of a fluorescent in situ hybridization (FISH) technique for the detection of Helicobacter pylori in water (river and wastewater) samples. The assay was compared with PCR detection and isolation of cells on selective media. 16S rRNA and UreA+B sequence data were used as oligonucleotide probe and specific primers for FISH and PCR, respectively. Using FISH technique, H. pylori was detected in two river water and one wastewater samples, while PCR yielded only one positive result. H. pylori culture was not possible from any sample. According to these results, FISH technique has the potential to be used as a quick and sensitive method for detection of H. pylori in environmental samples.
Moreno
Y
Y
Departamento de Biotecnologi;a, Universidad Politécnica, Camino de Vera 14, 46022 Valencia, Spain.
Ferrús
M A
MA
Alonso
J L
JL
Jiménez
A
A
Hernández
J
J
D004269:DNA, Bacterial / Q000032:analysis*; D004784:Environmental Monitoring / Q000379:methods*; D016480:Helicobacter pylori / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D012336:RNA, Ribosomal, 16S; D012680:Sensitivity and Specificity; D014871:Water Microbiology; D014881:Water Supply
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S