title: Imaging of immobilized enzyme spots by scanning chemiluminescence microscopy with electrophoretic injection.
abstract: Scanning chemiluminescence microscopy (SCLM) with electrophoretic injection was developed and applied to visualize enzyme reactions localized in an enzyme microspot. The SCLM uses a tapered glass capillary as a probe for injecting a small amount of luminol onto the substrate to generate localized chemiluminescence. The electrophoretic injection by application of a constant current between the inside and outside of the capillary enabled the continuous and controllable injection of a minute quantity of luminol in the range of 0.1 pmol/s. The image of enzyme activity in a monolayer spot of horseradish peroxidase was obtained by using the electrophoretic injection-based SCLM system.
authors.affiliation: Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, 07 Aramaki-Aoba, Aoba-ku, 980-8579, Sendai, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Hirano
authors.forename: Yutaro
authors.initials: Y
authors.lastname: Mitsumori
authors.forename: Daisuke
authors.initials: D
authors.lastname: Oyamatsu
authors.forename: Matsuhiko
authors.initials: M
authors.lastname: Nishizawa
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Matsue
mesh_terms: D004586:Electrophoresis / Q000379:methods*; D004800:Enzymes, Immobilized / Q000032:analysis / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000032:analysis* / Q000737:chemistry*; D008163:Luminescent Measurements; D008165:Luminol; D008845:Microinjections / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D010783:Photometry / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D004800:Enzymes, Immobilized; D008165:Luminol; D006735:Horseradish Peroxidase