The role of octadecanoids and functional mimics in soybean defense responses.
Oxylipins of the jasmonate pathway and synthetic functional analogs have been analyzed for their elicitor-like activities in an assay based on the induced accumulation of glyceollins, the phytoalexins of soybean (Glycine max L.), in cell suspension cultures of this plant. Jasmonic acid (JA) and its methyl ester showed weak phytoalexin-inducing activity when compared to an early jasmonate biosynthetic precursor, 12-oxo-phytodienoic acid (OPDA), as well as to the bacterial phytotoxin coronatine and certain 6-substituted indanoyl-L-isoleucine methyl esters, which all were highly active. Interestingly, different octadecanoids and indanoyl conjugates induced the accumulation of transcripts of various defense-related genes to different degrees, indicating distinct induction competencies. Therefore, these signaling compounds and mimics were further analyzed for their effects on signal transduction elements, such as the transient enhancement of the cytosolic Ca2+ concentration and MAP kinase activation, which are known to be initiated by a soybean pathogen-derived beta-glucan elicitor. In contrast to the beta-glucan elicitor, none of the other compounds tested triggered these early signaling elements. Moreover, endogenous levels of OPDA and JA in soybean cells were shown to be unaffected after treatment with beta-glucans. Thus, OPDA and JA, which are functionally mimicked by coronatine and a variety of 6-substituted derivatives of indanoyl-L-isoleucine methyl ester, represent highly efficient signaling compounds of a lipid-based pathway not deployed in the beta-glucan elicitor-initiated signal transduction.
Fliegmann
Judith
J
Department Biologie I der Ludwig-Maximilians-Universität, Botanik, Menzinger Str. 67, D-80638 München, Germany.
Schüler
Göde
G
Boland
Wilhelm
W
Ebel
Jürgen
J
Mithöfer
Axel
A
D000085:Acetates / Q000378:metabolism* / Q000494:pharmacology; D000596:Amino Acids / Q000494:pharmacology; D001578:Benzopyrans / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism* / Q000494:pharmacology; D005231:Fatty Acids, Unsaturated / Q000378:metabolism / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000502:physiology*; D007192:Indenes / Q000494:pharmacology; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D018716:Molecular Mimicry; D054883:Oxylipins; D010937:Plant Growth Regulators / Q000378:metabolism* / Q000494:pharmacology; D036343:Pterocarpans; D012333:RNA, Messenger / Q000032:analysis; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D013025:Glycine max / Q000201:enzymology / Q000378:metabolism / Q000502:physiology*
D000085:Acetates; D000596:Amino Acids; D001578:Benzopyrans; D003517:Cyclopentanes; D005231:Fatty Acids, Unsaturated; D007192:Indenes; D054883:Oxylipins; D010937:Plant Growth Regulators; D036343:Pterocarpans; D012333:RNA, Messenger; C058487:coronatine; C017343:glyceollin; C025999:12-oxophytodienoic acid; C011006:jasmonic acid; C072239:methyl jasmonate; D020928:Mitogen-Activated Protein Kinases