Variation in sequence-specific repair of UV damage in human pericentromeric heterochromatin of different cell lines.
Damaged nucleotides are removed from the condensed non-coding, or transcriptionally inactive regions of the genome by the relatively slow global genome repair system. Since few data are available for the repair of the pericentric heterochromatin region our aim was to study the repair of a specific sequence, known to be located in this region. We applied a PCR based method to monitor UV damage and repair in chAB4, a human pericentromeric heterochromatin sequence in 10 human cell lines. We here present evidence that excision repair of a sequence in the pericentromeric heterochomatin also varies between cell lines in a manner inconsistent with the canonical model. In some cell lines repair rates were efficient in heterochromatin, comparable to transcription coupled repair, but in some tumour-derived and repair-deficient cell lines we have detected deficient repair.
Mórocz
Mónika
M
Biological Research Centre of Hungarian Academy of Sciences, Institute of Genetics, POB 521, H-6701, Szeged, Hungary. morocz@nucleus.szbk.u-szeged.hu
Csiszár
Agnes
A
Johnson
Robert T
RT
Downes
C Stephen
CS
Cserpán
Imre
I
Raskó
István
I
D002087:Butyrates / Q000494:pharmacology; D002460:Cell Line; D002503:Centromere / Q000378:metabolism*; D004249:DNA Damage; D004260:DNA Repair; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts / Q000378:metabolism; D006367:HeLa Cells; D006570:Heterochromatin / Q000378:metabolism*; D006801:Humans; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013997:Time Factors; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays
D002087:Butyrates; D006570:Heterochromatin