title: Novel synthetic phytochelatin-based capacitive biosensor for heavy metal ion detection.
abstract: A novel capacitance biosensor based on synthetic phytochelatins for sensitive detection of heavy metals is described. Synthetic phytochelatin (Glu-Cys)(20)Gly (EC20) fused to the maltose binding domain protein was expressed in Escherichia coli and purified for construction of the biosensor. The new biosensor was able to detect Hg(2+), Cd(2+), Pb(2+), Cu(2+) and Zn(2+) ions in concentration range of 100 fM-10 mM, and the order of sensitivity was S(Zn)>S(Cu)>S(Hg)>>S(Cd) congruent with S(Pb). The biological sensing element of the sensor could be regenerated using EDTA and the storage stability of the biosensor was 15 days.
authors.affiliation: Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00, Lund, Sweden.
authors.forename: Ibolya
authors.initials: I
authors.lastname: Bontidean
authors.forename: Josefin
authors.initials: J
authors.lastname: Ahlqvist
authors.forename: Ashok
authors.initials: A
authors.lastname: Mulchandani
authors.forename: Wilfred
authors.initials: W
authors.lastname: Chen
authors.forename: Weon
authors.initials: W
authors.lastname: Bae
authors.forename: Rajesh K
authors.initials: RK
authors.lastname: Mehra
authors.forename: Alessia
authors.initials: A
authors.lastname: Mortari
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Csöregi
mesh_terms: D015374:Biosensing Techniques; D025802:Electric Capacitance; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005978:Glutathione; D008667:Metalloproteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D019216:Metals, Heavy / Q000032:analysis* / Q000737:chemistry*; D008832:Microchemistry / Q000295:instrumentation / Q000379:methods; D054811:Phytochelatins; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D008667:Metalloproteins; D019216:Metals, Heavy; D011993:Recombinant Fusion Proteins; D054811:Phytochelatins; D005978:Glutathione