Role of the F-box protein Skp2 in cell proliferation in the developing auditory system in mice.
The F-box protein, Skp2 positively regulates the G1-S transition by controlling the stability of several G1 regulators, including p27. To evaluate the role of Skp2 in the mammalian developing auditory systems, the expression of Skp2 was examined together with the expression of p27 in auditory systems. Our data show expression of Skp2 in auditory epithelia and neurons at an early stage of development. During differentiation processes, the onset of p27 expression was observed together with the down-regulation of Skp2 expression, in auditory epithelia. In contrast, an alteration of expression of p27 and Skp2 in the greater epithelial ridge and spiral ganglion appeared after differentiation of hair cells. These findings suggest that Skp2 plays a crucial role in development of mammalian auditory systems.
Dong
Youyi
Y
Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kawaharacho 54, Shogoin, Sakyo-ku, 606-8507 Kyoto, Japan.
Nakagawa
Takayuki
T
Endo
Tsuyoshi
T
Kim
Tae-Soo
TS
Iguchi
Fukuichiro
F
Yamamoto
Norio
N
Naito
Yasushi
Y
Ito
Juichi
J
D000818:Animals; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D003051:Cochlea / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D050760:Cyclin-Dependent Kinase Inhibitor p27; D004398:Dyneins; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D005865:Gestational Age; D006198:Hair Cells, Auditory / Q000166:cytology / Q000378:metabolism; D019394:Ki-67 Antigen / Q000096:biosynthesis; D051379:Mice; D008813:Mice, Inbred ICR; D000081412:Myosin VIIa; D009218:Myosins / Q000096:biosynthesis; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D044786:S-Phase Kinase-Associated Proteins; D013136:Spiral Ganglion / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000378:metabolism
C493961:Cdkn1b protein, mouse; D018797:Cell Cycle Proteins; D019394:Ki-67 Antigen; C000631456:Myo7a protein, mouse; D000081412:Myosin VIIa; D044786:S-Phase Kinase-Associated Proteins; D025521:Tumor Suppressor Proteins; D050760:Cyclin-Dependent Kinase Inhibitor p27; D009218:Myosins; D004398:Dyneins