title: Selective occlusion of lumbar arteries as a spinal cord ischemia model in rabbits.
abstract: Paraplegia is a devastating complication of operations requiring transient occlusion of the descending thoracic aorta. Many animal models of spinal cord ischemia have been utilized to examine the efficacy of various neuroprotective methods. In this study, we establish a rabbit model of spinal cord ischemia by selective temporary occlusion of lumbar arteries and examine the protective effects of systemic mild hypothermia in this model. Animals were divided into the following four groups: sham group (group A, n = 6); 10 min ischemia, normothermia (39 degrees C) (group B, n = 6); 20 min ischemia, normothermia (group C, n = 6); and 30 min ischemia, mild hypothermia (35 degrees C) (group D, n = 6). After 7 d of reperfusion, three rabbits in group B and five rabbits in group C developed paraplegia (Tarlov's score = 0). In contrast, all rabbits preserved hindlimb motor function (Tarlov's score = 4) in groups A and D. Histological findings indicated that the number of motor neurons in the anterior horns in group C were significantly fewer than in group A. A large number of motor neurons were preserved in group D. Hypothermia is known to be an effective and reliable method of neuroprotection, but the risk of complications rises at deep hypothermia. Our current results confirm that systemic, mild hypothermia is a safe and effective neuroprotective method during ischemia-reperfusion injury of the spinal cord.
authors.affiliation: Division of Adaptation Physiology, Faculty of Medicine, Tottori University, 86 Nishi-machi, Yonago, 683-8503 Japan.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Maeda
authors.forename: Koichi
authors.initials: K
authors.lastname: Mori
authors.forename: Yoshimitsu
authors.initials: Y
authors.lastname: Shiraishi
authors.forename: Kyoko
authors.initials: K
authors.lastname: Tatebayashi
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Kawai
mesh_terms: D000818:Animals; D001157:Arterial Occlusive Diseases / Q000150:complications / Q000175:diagnosis / Q000473:pathology; D017679:Cryotherapy / Q000379:methods*; D007036:Hypothermia, Induced / Q000379:methods*; D008159:Lumbar Vertebrae / Q000098:blood supply / Q000473:pathology / Q000601:surgery; D008297:Male; D023421:Models, Animal; D009474:Neurons / Q000473:pathology; D011817:Rabbits; D013116:Spinal Cord / Q000098:blood supply* / Q000601:surgery*; D020760:Spinal Cord Ischemia / Q000175:diagnosis / Q000209:etiology / Q000473:pathology* / Q000628:therapy*; D014711:Vertebral Artery / Q000601:surgery*