title: Lipid biosynthesis in developing perilla seeds.
abstract: In developing seeds of Perilla frutescens var. crispa, the triacylglycerol fraction was found to accumulate between 15 and 19 days after flowering. Of this, 65% of the total fatty acids was alpha-linolenic acid in the mature seeds, with the latter being esterified in comparable amounts at all positions (sn-1, 2 and 3) of the glycerol residue. It was also demonstrated that, 1-acylglycerol-3-phosphate acyltransferase, which catalyzes esterification at the sn-2 position of the glycerol backbone, showed low activities for alpha-linolenoyl-CoA as substrate. These findings suggest that the diacylglycerol precursor for triacylglycerol synthesis is not directly derived from phosphatidic acid through the glycerol phosphate pathway.
authors.affiliation: Biological Chemistry, Faculty of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto 606-8522, Japan. ichihara@kpu.ac.jp
authors.forename: Ken'ichi
authors.initials: K
authors.lastname: Ichihara
authors.forename: Yasumasa
authors.initials: Y
authors.lastname: Suda
mesh_terms: D051103:1-Acylglycerol-3-Phosphate O-Acyltransferase; D000214:Acyl Coenzyme A / Q000378:metabolism; D000217:Acyltransferases / Q000378:metabolism; D004951:Esterification; D005990:Glycerol / Q000737:chemistry / Q000378:metabolism; D008954:Models, Biological; D031343:Perilla / Q000378:metabolism*; D012639:Seeds / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism*; D013237:Stereoisomerism; D014280:Triglycerides / Q000096:biosynthesis*; D017962:alpha-Linolenic Acid / Q000737:chemistry / Q000378:metabolism
chemical_list: D000214:Acyl Coenzyme A; D014280:Triglycerides; D017962:alpha-Linolenic Acid; D000217:Acyltransferases; D051103:1-Acylglycerol-3-Phosphate O-Acyltransferase; D005990:Glycerol