New turf for CFP/YFP FRET imaging of membrane signaling molecules.
TIRF microscopy can be used in conjunction with CFP/YFP FRET to detect movements of the cytoplasmic tails of GIRK channels (Riven et al., this issue of Neuron). This innovative combination of techniques allows molecular resolution of small motions underlying ion channel activation by G proteins and will likely find widespread use for study of membrane-associated molecules.
Evans
Jenafer
J
The Johns Hopkins University School of Medicine, Department of Biomedical Engineering, Calcium Signals Laboratory, Ross 713, 720 Rutland Avenue, Baltimore, MD 21205, USA.
Yue
David T
DT
D001426:Bacterial Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000378:metabolism*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D015221:Potassium Channels / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology
D001426:Bacterial Proteins; D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D015221:Potassium Channels; C054562:yellow fluorescent protein, Bacteria; D049452:Green Fluorescent Proteins