Comparative analysis of proteome maps of Helicobacter pylori clinical isolates.
The gram-negative bacterium Helicobacter pylori is found in human gastric mucosa. A widely distributed H. pylori infection is associated with chronic gastritis, gastric and duodenal ulcers, and malignant neoplasms. In this study proteome maps of four H. pylori clinical isolates derived from patients of two Russian regions (Moscow/Moscow Region and Novosibirsk) were obtained using 2D-electrophoresis and MALDI-TOF-mass-spectrometry. Variability of some H. pylori proteins and the level of their expression have been evaluated. These four isolates could be easily subdivided into two equal groups characterized by the close proteome profiles and the isolate from Moscow Region and the isolate from Novosibirsk constituted one group. The present study demonstrates the potential of proteome technology, which can be employed together with genome and transcriptome studies for the multiparameter typing of clinical isolates of pathogenic microorganisms.
Govorun
V M
VM
Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, 119121, Russia. proteomics@ibmh.msk.su
Moshkovskii
S A
SA
Tikhonova
O V
OV
Goufman
E I
EI
Serebryakova
M V
MV
Momynaliev
K T
KT
Lokhov
P G
PG
Khryapova
E V
EV
Kudryavtseva
L V
LV
Smirnova
O V
OV
Toropyguine
I Yu
IY
Maksimov
B I
BI
Archakov
A I
AI
D002457:Cell Extracts / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D005838:Genotype; D016481:Helicobacter Infections / Q000382:microbiology*; D016480:Helicobacter pylori / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D010449:Peptide Mapping; D020543:Proteome; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D002457:Cell Extracts; D020543:Proteome