[IL-8 and T-lymphocytes expressing adhesion molecules LFA-1 (CD11a/CD18), Mac-1 (CD11b/CD18) Lsel in lower respiratory tract obstructive disease].
Chemokines and T-lymphocytes play an important role in lower respiratory tract inflammation. This study evaluated the concentration of IL-8 and count of T-lymphocytes expressing adhesion molecules LFA-1, Mac-1, Lsel and their correlation in patients with asthma and COPD in periods of exacerbation and clinical improvement (after seven days of anti-inflammatory treatment). In all subjects bronchoscopic examination with BAL procedure were done in exacerbation period and after seven days of treatment. The concentration of IL-8 was measured by ELISA, and the expression of adhesion molecules by biotin-streptavidin methods. The highest concentration of IL-8 was observed in asthma patients in clinical improvement period, and the highest count of T-lymphocytes was observed in patients with COPD in remission phase. Increased concentration of chemokines could have been influenced by type of treatment administered, especially beta 2-mimetics. The significant correlation observed in COPD patients between IL-8 concentration and counts of T-cells expressing LFA-1 (r = 0.44), Mac-1 (r = 0.49), Lsel (r = 0.42) in exacerbation period suggest a chemotactic influence of IL-8 on T-lymphocytes.
Chciałowski
Andrzej
A
Klinika Chorób Wewnetrznych, Pneumonologii i Alergologii, Centralnego Szpitala Klinicznego WAM, Warszawie.
Płusa
Tadeusz
T
Lubiński
Wojciech
W
Pirozyńska
Ewa
E
Stankiewicz
Wanda
W
Carewicz
Romuald
R
D000208:Acute Disease; D000328:Adult; D001249:Asthma / Q000188:drug therapy / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D019041:L-Selectin / Q000378:metabolism*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism*; D016177:Macrophage-1 Antigen / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D029424:Pulmonary Disease, Chronic Obstructive / Q000188:drug therapy / Q000276:immunology*; D013601:T-Lymphocytes / Q000378:metabolism*; D013997:Time Factors
D016209:Interleukin-8; D016169:Lymphocyte Function-Associated Antigen-1; D016177:Macrophage-1 Antigen; D019041:L-Selectin
IL-8 i limfocyty T wykazujace ekspresje czasteczek adhezyjnych LFA-1 (CD11a/CD18), Mac-1 (CD11b/CD18), Lsel w obturacyjnych chorobach dróg oddechowych.