Preoperative irradiation combined with chemotherapy impairs healing of bronchial anastomosis during the early postoperative period in rats.
The effect of preoperative irradiation and antineoplastic agents on healing at the site of bronchial anastomosis was investigated using rats. The bursting pressure in irradiation group and combined irradiation and chemotherapy group was significantly lower than in control and chemotherapy group at day 5 after operation. There was no significant difference in bursting pressure in all groups at day 7. The histologic finding of the anastomosis with H & E stain showed that submucosal connective tissue had not regenerated, and defects were seen in the submucosal tissue in irradiation and combined therapy group at day 3 and day 5. But, the connective tissue had matured in irradiation group at day 7 compared with control group. In conclusion, this study demonstrated that the healing of bronchial anastomosis was markedly delayed in early postoperative days in the rats receiving irradiation and combined therapy.
Inoue
Masao
M
Division of Surgical Oncology, Department of Translational Medical Sciences, Nagasaki University School Graduate School of Biomedical Sciences, Nagasaki 852-8501, Japan. maino@bronze.ocn.ne.jp
Oka
Tadayuki
T
Shima
Yoshikatsu
Y
Shirafuji
Tomoyuki
T
Sumida
Yorihisa
Y
Yamashita
Hideki
H
Muraoka
Masashi
M
Nagayasu
Takeshi
T
Akamine
Shinji
S
Shimokawa
Isao
I
Ayabe
Hiroyoshi
H
D000714:Anastomosis, Surgical; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D001835:Body Weight / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D001980:Bronchi / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects*; D002945:Cisplatin / Q000494:pharmacology; D005047:Etoposide / Q000494:pharmacology; D006909:Hydroxyproline / Q000378:metabolism; D007958:Leukocyte Count; D008297:Male; D011312:Pressure; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing / Q000187:drug effects* / Q000528:radiation effects*
D000970:Antineoplastic Agents; D000972:Antineoplastic Agents, Phytogenic; D011838:Radiation-Sensitizing Agents; D005047:Etoposide; D002945:Cisplatin; D006909:Hydroxyproline