Intracellular activity of fosfomycin against two distinct enteropathogenic bacteria, Salmonella enterica and Listeria monocytogenes, alive inside host cells.
We studied the effect of fosfomycin (FOM) on the intracellular growth of two different facultative intracellular bacteria, Salmonella enterica serovar Typhimurium and Listeria monocytogenes, in an enterocyte-like cell line, Caco-(2). These bacteria replicate in different compartments within the host cells; Salmonella serovar Typhimurium grow inside phagosomes, whereas L. monocytogenes escape the phagosomal environment and multiply in the cytosol of the host cells. At concentrations equal to 0.25 of the MIC and 4 times the MIC, respectively, FOM effectively decreased the number of intracellular Salmonella serovar Typhimurium. Although FOM was ineffective at inhibiting the extracellular growth of L. monocytogenes in vitro, this antibiotic induced a marked reduction in intracellular L. monocytogenes, indicating that, comparatively, FOM may be more effectively taken up by L. monocytogenes in the intracellular environment.
Okada
Nobuhiko
N
Department of Microbiology, School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan. okadan@pharm.kitasato-u.ac.jp
Nishio
Miki
M
Danbara
Hirofumi
H
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018938:Caco-2 Cells; D003600:Cytosol / Q000382:microbiology; D020895:Enterocytes / Q000382:microbiology*; D005578:Fosfomycin / Q000494:pharmacology*; D006801:Humans; D007424:Intracellular Fluid / Q000382:microbiology; D008089:Listeria monocytogenes / Q000187:drug effects*; D008088:Listeriosis / Q000188:drug therapy; D008826:Microbial Sensitivity Tests; D010588:Phagosomes / Q000382:microbiology; D012480:Salmonella Infections / Q000188:drug therapy; D019779:Salmonella enterica / Q000187:drug effects*; D014407:Tumor Cells, Cultured
D000900:Anti-Bacterial Agents; D005578:Fosfomycin