Conventional detection method of fibrinogen and fibrin degradation products using latex piezoelectric immunoassay.
We developed a conventional immunosensor for fibrinogen and fibrin degradation products (FDP) to combine a quartz crystal microbalance (QCM) with the agglutination reaction of immunized latex beads. FDP induced an immunoreaction due to anti-FDP antibody immobilized latex particles. We successfully measured FDP concentration of in human serum within 10 min by QCM method. The detection range of QCM immunosensor is covered with screening concentration of FDP in serum (<10 microg/ml of FDP). The time course of latex agglutination obtained from QCM immunosensor is synchronized to that of latex photometric immunoassay. SEM was used to observe the surface of QCM that applied FDP serum. The size of latex particles agglutinated on the QCM electrode increased concomitant with FDP concentration. Frequency shift on immunoreaction explains the increased adsorption amount of agglutinated latex on QCM.
Aizawa
Hidenobu
H
National Institute of Advanced Industrial Science and Technology (AIST), 1-1 Higashi, Tsukuba, 305-8565, Ibaraki, Japan.
Kurosawa
Shigeru
S
Tozuka
Mitsuhiro
M
Park
Jong-Won
JW
Kobayashi
Koichi
K
Tanaka
Hideo
H
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D001774:Blood Chemical Analysis / Q000295:instrumentation* / Q000379:methods; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D019544:Equipment Failure Analysis; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D008863:Microspheres; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013499:Surface Properties
D020099:Coated Materials, Biocompatible; D005338:Fibrin Fibrinogen Degradation Products