Interaction between two cis-acting elements, ABRE and DRE, in ABA-dependent expression of Arabidopsis rd29A gene in response to dehydration and high-salinity stresses.
Many abiotic stress-inducible genes contain two cis-acting elements, namely a dehydration-responsive element (DRE; TACCGACAT) and an ABA-responsive element (ABRE; ACGTGG/TC), in their promoter regions. We precisely analyzed the 120 bp promoter region (-174 to -55) of the Arabidopsis rd29A gene whose expression is induced by dehydration, high-salinity, low-temperature, and abscisic acid (ABA) treatments and whose 120 bp promoter region contains the DRE, DRE/CRT-core motif (A/GCCGAC), and ABRE sequences. Deletion and base substitution analyses of this region showed that the DRE-core motif functions as DRE and that the DRE/DRE-core motif could be a coupling element of ABRE. Gel mobility shift assays revealed that DRE-binding proteins (DREB1s/CBFs and DREB2s) bind to both DRE and the DRE-core motif and that ABRE-binding proteins (AREBs/ABFs) bind to ABRE in the 120 bp promoter region. In addition, transactivation experiments using Arabidopsis leaf protoplasts showed that DREBs and AREBs cumulatively transactivate the expression of a GUS reporter gene fused to the 120 bp promoter region of rd29A. These results indicate that DRE and ABRE are interdependent in the ABA-responsive expression of the rd29A gene in response to ABA in Arabidopsis.
Narusaka
Yoshihiro
Y
Biological Resources Division, Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, Tsukuba, Ibaraki 305-8686, Japan.
Nakashima
Kazuo
K
Shinwari
Zabta K
ZK
Sakuma
Yoh
Y
Furihata
Takashi
T
Abe
Hiroshi
H
Narusaka
Mari
M
Shinozaki
Kazuo
K
Yamaguchi-Shinozaki
Kazuko
K
D000040:Abscisic Acid / Q000494:pharmacology*; D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000378:metabolism; D001483:Base Sequence; D003681:Dehydration / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017930:Genes, Reporter / Q000235:genetics; D009154:Mutation / Q000235:genetics; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D020218:Response Elements / Q000235:genetics*; D012965:Sodium Chloride / Q000494:pharmacology*; D014157:Transcription Factors / Q000378:metabolism; D015533:Transcriptional Activation / Q000187:drug effects
D029681:Arabidopsis Proteins; C114578:DREB1A protein, Arabidopsis; D014157:Transcription Factors; D012965:Sodium Chloride; D000040:Abscisic Acid