title: Coordinate regulation of Salmonella enterica serovar Typhimurium invasion of epithelial cells by the Arp2/3 complex and Rho GTPases.
abstract: Salmonella enterica serovar Typhimurium can infect epithelial cells via the basolateral surface after breaching the intestinal epithelium, yet little is known about this process. Here, we show that actin polymerization driven by the Arp2/3 complex is critical to both basolateral and apical bacterial invasion of polarized MDCK cells. While there is also a dependence upon toxin B-sensitive Rho GTPases, none of the four GTPases known to be activated by S. enterica serovar Typhimurium SopE are individually required for basolateral internalization. These results underscore that the specific factors required for Salmonella invasion differ between membrane domains of polarized epithelia.
authors.affiliation: Department of Cell Biology, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908-0732,USA.
authors.forename: Alison K
authors.initials: AK
authors.lastname: Criss
authors.forename: James E
authors.initials: JE
authors.lastname: Casanova
mesh_terms: D051377:Actin-Related Protein 2; D051378:Actin-Related Protein 3; D000199:Actins / Q000378:metabolism; D000818:Animals; D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000494:pharmacology; D002460:Cell Line; D016764:Cell Polarity; D003598:Cytoskeletal Proteins / Q000502:physiology*; D004285:Dogs; D004847:Epithelial Cells / Q000382:microbiology; D012486:Salmonella typhimurium / Q000472:pathogenicity*; D020741:rho GTP-Binding Proteins / Q000502:physiology*
chemical_list: D051377:Actin-Related Protein 2; D051378:Actin-Related Protein 3; D000199:Actins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D003598:Cytoskeletal Proteins; C031815:toxB protein, Clostridium difficile; D020741:rho GTP-Binding Proteins