Determination of procaine hydrochloride using flow injection inhibitory chemiluminescence.
A new flow injection chemiluminescent method has been developed for the determination of procaine hydrochloride, based on the inhibition of the chemiluminescence reaction of luminol-hydrogen peroxide by procaine hydrochloride. The influence of several surfactants and beta-cyclodextrin on the chemiluminescence intensity were studied. It was found that beta-cyclodextrin enhanced the decrease in chemiluminescence intensity. The method is simple, convenient and sensitive, with a detection limit (3 sigma) of 0.08 micro g/mL. The decreased chemiluminescence intensity is linear, with the concentration of procaine hydrochloride in the range 0.2-100.0 micro g/mL and 100.0-400.0 micro g/mL. The relative standard deviation for 10 repeated measurements were 4.5% and 3.4% for 1.0 and 20.0 micro g/mL procaine hydrochloride, respectively. The method has been successfully applied to the determination of procaine hydrochloride in injection solutions of this drug.
Li
Nianbing
N
Department of Chemistry, Fuzhou University, Fuzhou 350002, People's Republic of China.
Chi
Yuwu
Y
Wang
Jian
J
Duan
Jianping
J
Chen
Guonan
G
D000779:Anesthetics, Local / Q000032:analysis*; D002713:Chlorine / Q000737:chemistry; D003505:Cyclodextrins / Q000737:chemistry; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods; D006861:Hydrogen Peroxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry; D011343:Procaine / Q000031:analogs & derivatives / Q000032:analysis*; D015203:Reproducibility of Results; D013501:Surface-Active Agents / Q000737:chemistry; D047392:beta-Cyclodextrins
D000779:Anesthetics, Local; D003505:Cyclodextrins; D013501:Surface-Active Agents; D047392:beta-Cyclodextrins; D002713:Chlorine; D011343:Procaine; D008165:Luminol; D006861:Hydrogen Peroxide; C031215:betadex