Oxidative damage in colon and mammary tissue of the HFE-knockout mouse.
The HFE mutation is common and, when homozygous, can lead to a morbid accumulation of body iron and the disease hereditary hemochromatosis. Heterozygotes compose 10-15% of the European-American population, and have evidence of elevated body iron compared to homozygous normal people. Dietary iron content was hypothesized to interact with the HFE genotype to influence oxidative damage in mammary and colon tissue. Two groups of HFE-knockout mice were fed a standard iron diet (300 ppm) or a low iron diet (30 ppm). There was a significantly elevated concentration of malondialdehyde (by HPLC) in mammary (305 pmol/g vs. 166, p =.04) and colon (349 pmol/g vs. 226, p =.02) tissue among those mice on the standard iron diet compared to those on the low iron diet. These results suggest that dietary modification may affect the course of iron overload from HFE mutations.
Stevens
Richard G
RG
Department of Community Medicine, University of Connecticut Health Center, Farmington 06030, USA. bugs@neuron.uchc.edu
Morris
James E
JE
Cordis
Gerald A
GA
Anderson
Larry E
LE
Rosenberg
Daniel W
DW
Sasser
Lyle B
LB
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003106:Colon / Q000378:metabolism* / Q000473:pathology; D017353:Gene Deletion; D000071020:Hemochromatosis Protein; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000378:metabolism; D007501:Iron / Q000008:administration & dosage / Q000032:analysis / Q000494:pharmacology; D019266:Iron, Dietary / Q000008:administration & dosage / Q000494:pharmacology; D008315:Malondialdehyde / Q000032:analysis / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000378:metabolism* / Q000473:pathology; D008565:Membrane Proteins / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D018384:Oxidative Stress
D000071020:Hemochromatosis Protein; C499535:Hfe protein, mouse; D015395:Histocompatibility Antigens Class I; D019266:Iron, Dietary; D008565:Membrane Proteins; D008315:Malondialdehyde; D007501:Iron