title: Essentiality of clpX, but not clpP, clpL, clpC, or clpE, in Streptococcus pneumoniae R6.
abstract: We show by using a regulated promoter that clpX of Streptococcus pneumoniae R6 is essential, whereas clpP, clpL, clpC, and clpE can be disrupted. The essentiality of clpX was initially missed because of duplication and rearrangement in the region of the chromosome containing clpX. Depletion of ClpX resulted in a rapid loss of viability without overt changes in cell morphology. Essentiality of clpX, but not clpP, has not been reported previously.
authors.affiliation: Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285, USA.
authors.forename: Gregory T
authors.initials: GT
authors.lastname: Robertson
authors.forename: Wai-Leung
authors.initials: WL
authors.lastname: Ng
authors.forename: Raymond
authors.initials: R
authors.lastname: Gilmour
authors.forename: Malcolm E
authors.initials: ME
authors.lastname: Winkler
mesh_terms: D000074183:ATPases Associated with Diverse Cellular Activities; D000251:Adenosine Triphosphatases / Q000235:genetics*; D049071:Endopeptidase Clp; D029968:Escherichia coli Proteins; D017353:Gene Deletion; D005798:Genes, Bacterial / Q000502:physiology*; D018832:Molecular Chaperones; D013296:Streptococcus pneumoniae / Q000235:genetics / Q000502:physiology*; D013696:Temperature
chemical_list: D029968:Escherichia coli Proteins; D018832:Molecular Chaperones; D049071:Endopeptidase Clp; D000251:Adenosine Triphosphatases; C088072:ClpX protein, E coli; D000074183:ATPases Associated with Diverse Cellular Activities