title: A metal dispersed sol-gel biocomposite amperometric glucose biosensor.
abstract: A new glucose biosensor has been fabricated by immobilizing glucose oxidase into a copper dispersed sol-gel derived ceramic-graphite composite. The copper in the biocomposite offers excellent electrocatalytic activity towards the reduction (at -0.2 V) as well as oxidation (at +0.45 V) of hydrogen peroxide liberated in the enzymatic reaction enabling sensitive and selective determination of glucose. A linear response to glucose in the concentration range between 2.7 x 10(-5) to 4.0 x 10(-3) M with a correlation coefficient of 0.9987 and 4.0 x 10(-5) to 5.6 x 10(-3) M with a correlation coefficient of 0.9989 were observed with the electrocatalytic reduction and oxidation, respectively. Ascorbic acid and uric acid did not interfere with the glucose measurement during catalytic reduction at -0.2 V, a Nafion membrane was used to eliminate these interferences during the electrocatalytic oxidation at +0.45 V. The combination of copper catalysis and the promising feature of sol-gel biocomposite favor the sensitive and selective determination of glucose with improved analytical capabilities.
authors.affiliation: Satellite Venture Business Laboratory, Utsunomiya University, Yoto 7-1-2, 321-8585, Utsunomiya, Japan.
authors.forename: D
authors.initials: D
authors.lastname: Ravi Shankaran
authors.forename: N
authors.initials: N
authors.lastname: Ueheara
authors.forename: T
authors.initials: T
authors.lastname: Kato
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002516:Ceramics / Q000737:chemistry; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis* / Q000737:chemistry; D003300:Copper / Q000737:chemistry*; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry; D019544:Equipment Failure Analysis; D005465:Fluorocarbon Polymers / Q000737:chemistry; D005782:Gels / Q000737:chemistry; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry*; D006108:Graphite; D006861:Hydrogen Peroxide / Q000032:analysis / Q000737:chemistry; D017646:Organosilicon Compounds; D044367:Phase Transition; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012821:Silanes / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D004800:Enzymes, Immobilized; D005465:Fluorocarbon Polymers; D005782:Gels; D017646:Organosilicon Compounds; D012821:Silanes; C040402:perfluorosulfonic acid; D006108:Graphite; D003300:Copper; C102833:(3-mercaptopropyl)trimethoxysilane; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose