Peptide-binding assessment using mass spectrometry as a new screening method for skin sensitization.
Skin sensitization potential of low molecular weight chemicals was assessed by analyzing peptide-conjugate formation. Chemicals were incubated with a peptide, glutathione, and resultant mixtures were analyzed by mass spectrometry. Eighteen chemicals were assessed, and new peaks corresponding to chemical-peptide conjugates were detected for 13 of 14 known sensitizers. Conjugates were not detected for 4 negative chemicals. The method has advantages as a simple screening assay for assessing the sensitization potential of chemicals.
Kato
Hiroshi
H
Environmental Health Science Laboratory, Sumitomo Chemical Co., Ltd., 3-1-98 Kasugadenaka, Konohana-ku, Osaka 554-8558, Japan.
Okamoto
Masahiko
M
Yamashita
Kazuko
K
Nakamura
Yosuke
Y
Fukumori
Yoshihiro
Y
Nakai
Kiyoshi
K
Kaneko
Hideo
H
D000818:Animals; D017449:Dermatitis, Allergic Contact / Q000175:diagnosis*; D004137:Dinitrochlorobenzene / Q000032:analysis / Q000737:chemistry; D005978:Glutathione / Q000032:analysis / Q000737:chemistry; D006168:Guinea Pigs; D013058:Mass Spectrometry; D010455:Peptides / Q000032:analysis / Q000737:chemistry; D011485:Protein Binding / Q000187:drug effects*; D023422:Skin Irritancy Tests / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014118:Toxins, Biological / Q000009:adverse effects / Q000032:analysis / Q000737:chemistry; D014302:Trinitrobenzenesulfonic Acid / Q000032:analysis / Q000737:chemistry
D004137:Dinitrochlorobenzene; D010455:Peptides; D014118:Toxins, Biological; D014302:Trinitrobenzenesulfonic Acid; D005978:Glutathione