title: [Expression of CXCR4 and its effect on the biological behavior of multiple myeloma cells].
abstract: OBJECTIVE
  To investigate the effect of stromal cell derived factor-1alpha(SDF-1alpha) expression and its receptor CXCR4 on the biological behavior of multiple myeloma (MM) cells and on the expression of soluble intercellular adhesion molecule 1 (ICAM-1).
  
  
  METHODS
  FACS analysis was used to study the expression of ICAM-1 (CD(54)) and CXCR4 on the surface of MM cells. Chemotaxis assay through transwell bore polycaronate and ELISA assay were employed to monitor the soluble ICAM-1 level.
  
  
  RESULTS
  (1) Fresh MM cells expressed variable levels of functional CXCR4 [(50.4 +/- 27.3)%], which was correlated with the in vitro ability of transwell migration of MM cells [(23.6 +/- 17.2)%, P < 0.01]. (2) SDF-1alpha could up-regulate the expression of ICAM-1 on MM cells. Furthermore, the serum level of sICAM-1 was correlated with the expression of CXCR4 on MM cells.
  
  
  CONCLUSIONS
  SDF-1alpha/CXCR4 plays an important role on the biological behavior of MM cells via mediating the effect of adhesion molecules.
authors.affiliation: Suzhou Medical Biotechnology Institute, Suzhou University, Suzhou 215007, China.
authors.forename: Cai-Xia
authors.initials: CX
authors.lastname: Li
authors.forename: De-Pei
authors.initials: DP
authors.lastname: Wu
authors.forename: Yue-Dan
authors.initials: YD
authors.lastname: Wang
authors.forename: Ge-Hua
authors.initials: GH
authors.lastname: Yu
authors.forename: Ji-Ming
authors.initials: JM
authors.lastname: Liu
authors.forename: Yu-Mei
authors.initials: YM
authors.lastname: Zhuang
authors.forename: Chang-Shuo
authors.initials: CS
authors.lastname: Yin
authors.forename: Xue-Guang
authors.initials: XG
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D002465:Cell Movement; D054377:Chemokine CXCL12; D019743:Chemokines, CXC / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism / Q000473:pathology; D009101:Multiple Myeloma / Q000378:metabolism* / Q000473:pathology; D019718:Receptors, CXCR4 / Q000096:biosynthesis* / Q000502:physiology; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
chemical_list: C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D019743:Chemokines, CXC; D019718:Receptors, CXCR4; D018799:Intercellular Adhesion Molecule-1