[Expression of gelatinase A and B in cord blood CD34+ cells and leukemic cell lines].
OBJECTIVE
To study the expression of gelatinases, including matrix metalloproteinase-9 (MMP-9, gelatinase B) and matrix metalloproteinase-2 (MMP-2, gelatinase A), in CD(34)(+) cells and leukemic cell lines, and explore the significance of gelatinase in migrating and homing capacity of CD(34)(+) cells, as well as the role of gelatinase in leukemia pathogenesis.


METHODS
CD(34)(+) cells were isolated from umbilical cord blood and normal bone marrow by Mini MACS system. By zymogram analysis, MMP-2 and MMP-9 were detected in the serum free condition medium of CD(34)(+) cells and cell lines.


RESULTS
One brilliant band with molecular weight of 92 x 10(3) was detected in condition medium of cord blood CD(34)(+) cells. No band was detected in condition medium of bone marrow CD(34)(+) cells. Brilliant bands with molecular weight of 92 x 10(3) and 72 x 10(3) were detected in the condition medium of U937, KG-1a and HL-60 cell lines, but not in that of HEL, Namalva, CEM, K562 and LCL-H cell lines. In the condition media of J6-1 and J6-2 cells only the 92 x 10(3) band was detected.


CONCLUSIONS
Cord blood CD(34)(+) cells produced MMP-9, but bone marrow CD(34)(+) cells did not, partly explains the fact that cord blood CD(34)(+) cells possessed higher migrating capacity in comparison with bone marrow CD(34)(+) cells. The expression of MMP-9 and MMP-2 in leukemic cell lines varied.
Rao
Qing
Q
Institute of Hematology, CAMS and PUMC, Tianjin 300020, China.
Geng
Yi-qi
YQ
An
Li-li
LL
Wu
Ke-fu
KF
D018952:Antigens, CD34 / Q000032:analysis*; D017077:Culture Media, Conditioned / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005312:Fetal Blood / Q000166:cytology / Q000201:enzymology* / Q000276:immunology; D018922:HL-60 Cells; D006801:Humans; D020014:K562 Cells; D007938:Leukemia / Q000201:enzymology* / Q000473:pathology; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis*; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured; D020298:U937 Cells
D018952:Antigens, CD34; D017077:Culture Media, Conditioned; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9