Transcriptional repression of ADH2-regulated beta-xylanase production by ethanol in recombinant strains of Saccharomyces cerevisiae.
The regulation of endo-beta-(1,4)-xylanase production by two different strains of Saccharomyces cerevisiae, each transformed with the XYN2 gene from Trichoderma reesei under control of the promoter of the alcohol dehydrogenase II (ADH2) gene of S. cerevisiae, was investigated. In batch culture, the rate of xylanase production was severely reduced by the pulse addition of 390 mmol ethanol l(-1). Pulses of 190-630 mmol ethanol l(-1) into aerobic glucose-limited steady-state continuous cultures reduced the xylanase activity about five-fold and showed that ethanol repressed the ADH2 promoter, as was evident from Northern blot analyses. Derepression of the ADH2-regulated xylanase gene occurred at ethanol concentrations below approximately 50 mmol l(-1).
du Preez
J C
JC
Department of Microbiology and Biochemistry, University of the Free State, P.O. Box 339, 9300 Bloemfontein, South Africa. dpreezjc@sci.uovs.ac.za
Maré
J E
JE
Albertyn
J
J
Kilian
S G
SG
D000426:Alcohol Dehydrogenase / Q000378:metabolism*; D003470:Culture Media; D043364:Endo-1,4-beta Xylanases; D000431:Ethanol / Q000494:pharmacology*; D015966:Gene Expression Regulation, Fungal; D005947:Glucose / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D014158:Transcription, Genetic; D014242:Trichoderma / Q000201:enzymology / Q000235:genetics; D014995:Xylosidases / Q000235:genetics / Q000378:metabolism*
D003470:Culture Media; D011994:Recombinant Proteins; D000431:Ethanol; D000426:Alcohol Dehydrogenase; D014995:Xylosidases; D043364:Endo-1,4-beta Xylanases; D005947:Glucose