title: [Nitrate assimilation activity of yellow lupine root nodules ?].
abstract: Low nitrate assimilation activity of the root nodules was demonstrated by assaying the nitrate reductase, glutamate synthase, glutamate dehydrogenase, and asparagine synthase activities, as well as the kinetics of 14C-labeled saccharose incorporation in the amino acids and amides of the cortex and the bacteroid-containing root nodule zones. Irrespective of the exogenous nitrogen concentration (0, 11.2, or 25 mM NO3-), nitrate concentration in the nodules was low as compared to the plant roots, leaves, and stems. This allowed us to propose the presence of structural and/or metabolic barriers in the nodules limiting nitrate accessibility and assimilation.
authors.affiliation: Timiryazev Institute of Plant Physiology, Russian Academy of Science, Botanicheskaya ul. 35, Moscow, 127176 Russia.
authors.forename: S F
authors.initials: SF
authors.lastname: Izmaĭlov
authors.forename: R K
authors.initials: RK
authors.lastname: Bruskova
authors.forename: S A
authors.initials: SA
authors.lastname: Chechetka
authors.forename: S V
authors.initials: SV
authors.lastname: Kirnos
authors.forename: T A
authors.initials: TA
authors.lastname: Nikiforova
authors.forename: M V
authors.initials: MV
authors.lastname: Satskaya
mesh_terms: D000596:Amino Acids / Q000096:biosynthesis; D002250:Carbon Radioisotopes / Q000378:metabolism; D031297:Lupinus / Q000378:metabolism*; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism*; D018547:Plant Stems / Q000378:metabolism; D035281:Plant Tubers / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism
chemical_list: D000596:Amino Acids; D002250:Carbon Radioisotopes; D009566:Nitrates; D000644:Quaternary Ammonium Compounds; D013395:Sucrose; D009565:Nitrate Reductases; D050901:Nitrate Reductase; D009584:Nitrogen
vernacular_title: Nitratassimiliruiushchaia funktsiia kluben'kov liupina zheltogo ?.