Sequence of a cDNA encoding acetylcholinesterase from susceptible and resistant two-spotted spider mite, Tetranychus urticae.
Acetylcholinesterase (AChE) from two-spotted spider mites, Tetranychus urticae was compared between an organophosphate susceptible (TKD) and a resistant (NCN) strain. The AChE of TKD had lower affinity to acetylthiocholine and propionylthiocholine than that of NCN, and the inhibition of AChE by DDVP, ambenonium, eserine and n-methyl-eserine showed that NCN was more insensitive than TKD. AChE cDNA sequence was determined, and the 687 amino acids of primary structure were deduced. There were six replacements of amino acid residues in TKD and two in NCN. #F331(439)C was the only substitution unique to NCN, however, this mutation existed homozygously in only two out of nine mites. This residue is one of the gorge lining components, and #F331(439)C might act an important role in the sensitivity of AChE to the inhibitors.
Anazawa
Yoshio
Y
Institute of Agriculture and Forestry, University of Tsukuba, Ibaraki, Tenodai Tsukuba 305-8572, Japan.
Tomita
Takashi
T
Aiki
Yasuhiko
Y
Kozaki
Toshinori
T
Kono
Yoshiaki
Y
D000110:Acetylcholinesterase / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D004792:Enzyme Precursors / Q000737:chemistry / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics; D007305:Insecticide Resistance / Q000235:genetics; D007306:Insecticides; D008925:Mites / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D009943:Organophosphorus Compounds; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014101:Torpedo
D018076:DNA, Complementary; D004792:Enzyme Precursors; D007306:Insecticides; D009943:Organophosphorus Compounds; D000110:Acetylcholinesterase