title: Influence of testosterone on regulation of ODC, antizyme, and N1-SSAT gene expression in mouse kidney.
abstract: Polyamines are involved in the control of the cell cycle and cell growth. In murine kidney, testosterone enhances gene expression of ornithine decarboxylase (ODC), the first enzyme in polyamine biosynthesis. In this study, we document the time course effect of testosterone on 1) gene expression of ODC, antizyme 1 (AZ1), and spermidine/spermine-N1-acetyltransferase (N1-SSAT); 2) ODC activity in proximal convoluted tubules (PCT) and cortical proximal straight tubules (CPST); and 3) renal polyamine levels. Female mice were treated with testosterone for a period of 1, 2, 3, and 5 consecutive days. ODC gene expression was extremely low in kidneys of untreated female mice compared with that of males. Consequently, the renal putrescine level was sevenfold lower in females than in males, whereas spermidine and spermine levels did not differ between sexes. In female kidneys, testosterone treatment sharply increased ODC mRNA and protein levels as well as ODC activity. Testosterone increased the expression of ODC in PCT and CPST over different time courses, which suggests that ODC activity is differentially regulated in distinct tubules. The expression of AZ1 and N1-SSAT mRNA was similar in male and female mouse kidneys. Testosterone treatment enhanced AZ1 and N1-SSAT mRNA levels in a time-dependent manner by unknown molecular mechanisms. Putrescine and spermidine levels increased after testosterone treatment in female kidneys. Surprisingly, although ODC protein and activity were undetectable in female kidneys, the levels of AZ1 mRNA and protein were similar to those in males. Therefore, one may propose that ODC protein could be continuously degraded by AZ1 in female kidneys.
authors.affiliation: Laboratoire de Physiopathologie Métabolique et Rénale, Faculté de Médecine Lyon RTH Laënnec, Institut National de la Santé et de la Recherche Médicale, Unite 499, Lyon, France. Olivier.Levillain@laennec.univ-lyon1.fr
authors.forename: Olivier
authors.initials: O
authors.lastname: Levillain
authors.forename: Anna
authors.initials: A
authors.lastname: Greco
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Diaz
authors.forename: Roger
authors.initials: R
authors.lastname: Augier
authors.forename: Anne
authors.initials: A
authors.lastname: Didier
authors.forename: Karine
authors.initials: K
authors.lastname: Kindbeiter
authors.forename: Frédéric
authors.initials: F
authors.lastname: Catez
authors.forename: Myriam
authors.initials: M
authors.lastname: Cayre
mesh_terms: D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D007684:Kidney Tubules / Q000737:chemistry / Q000187:drug effects* / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D051379:Mice; D009955:Ornithine Decarboxylase / Q000235:genetics / Q000378:metabolism*; D011073:Polyamines / Q000032:analysis; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012727:Sex Characteristics; D013739:Testosterone / Q000494:pharmacology*; D013997:Time Factors; D000099228:Diamine N-Acetyltransferase
chemical_list: D000123:Acetyltransferases; D009955:Ornithine Decarboxylase; D011073:Polyamines; D011506:Proteins; D012333:RNA, Messenger; D013739:Testosterone; D000099228:Diamine N-Acetyltransferase; C031017:ornithine decarboxylase antizyme