Surface modification of poly(glycolic acid) (PGA) for biomedical applications.
The immobilization of biological ligands (such as biotin and peptides) onto biodegradable polymer surfaces, including poly(glycolic acid) (PGA) sutures, is complicated by the absence of functional groups on the polymer backbone. We demonstrate a method for overcoming this problem, by attaching (+)-biotinyl-3,6,9-trioxaundecanediamine to the surface of PGA sutures, which immobilizes the ligand through an amide bond between amine (ligands) and carboxylic acid groups (surface-hydrolyzed PGA sutures). Fluorescence microscopy was used to verify the attachment of the biotin ligand to the surface of the PGA suture after a complexation with fluorescein-conjugated streptavidin. The strategy can be generalized to surface modifications of other biodegradable aliphatic polyesters, which would improve the properties of the polymers in biomedical applications such as active targeting of drugs based on ligand-attached, polymeric drug delivery systems.
Lee
Kyung-Bok
KB
Department of Chemistry, School of Molecular Science-BK21, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea.
Yoon
Kuk Ro
KR
Woo
Seong Ihl
SI
Choi
Insung S
IS
D001673:Biodegradation, Environmental; D001710:Biotin / Q000031:analogs & derivatives / Q000737:chemistry*; D016503:Drug Delivery Systems; D006868:Hydrolysis; D008024:Ligands; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D011100:Polyglycolic Acid / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry; D013499:Surface Properties; D013537:Sutures
D008024:Ligands; C480527:biotinyl-3,6,9-trioxaundecanediamine; D011100:Polyglycolic Acid; D001710:Biotin; D019809:Streptavidin