title: Expression of a luxS gene is not required for Borrelia burgdorferi infection of mice via needle inoculation.
abstract: The luxS gene product is an integral component of LuxS/autoinducer-2 (AI-2) quorum-sensing systems in bacteria. A putative luxS gene was expressed at comparable levels by Borrelia burgdorferi strain 297 cultivated either in vitro or in dialysis membrane chambers implanted in rat peritoneal cavities. Although the borrelial luxS gene functionally complemented a LuxS deficiency in Escherichia coli DH5 alpha, AI-2-like activity could not be detected within B. burgdorferi culture supernatants or concentrated cell lysates. Finally, a luxS-deficient mutant of B. burgdorferi was infectious at wild-type levels when it was intradermally needle inoculated into mice, indicating that expression of luxS probably is not required for infectivity but, at the very least, is not essential for mammalian host adaptation. Our findings also challenge the notion that a LuxS/AI-2 quorum-sensing system is operative in B. burgdorferi.
authors.affiliation: Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.
authors.forename: Anette
authors.initials: A
authors.lastname: Hübner
authors.forename: Andrew T
authors.initials: AT
authors.lastname: Revel
authors.forename: Dena M
authors.initials: DM
authors.lastname: Nolen
authors.forename: Kayla E
authors.initials: KE
authors.lastname: Hagman
authors.forename: Michael V
authors.initials: MV
authors.lastname: Norgard
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology; D025065:Borrelia burgdorferi / Q000235:genetics* / Q000472:pathogenicity; D013437:Carbon-Sulfur Lyases; D005798:Genes, Bacterial / Q000502:physiology*; D008193:Lyme Disease / Q000209:etiology*; D051379:Mice; D009154:Mutation; D009339:Needles; D009876:Operon; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D013437:Carbon-Sulfur Lyases; C117753:LuxS protein, Bacteria