Production of mannitol and lactic acid by fermentation with Lactobacillus intermedius NRRL B-3693.
Lactobacillus intermedius B-3693 was selected as a good producer of mannitol from fructose after screening 72 bacterial strains. The bacterium produced mannitol, lactic acid, and acetic acid from fructose in pH-controlled batch fermentation. Typical yields of mannitol, lactic acid, and acetic acid from 250 g/L fructose were 0.70, 0.16, and 0.12 g, respectively per g of fructose. The fermentation time was greatly dependent on fructose concentration but the product yields were not dependent on fructose level. Fed-batch fermentation decreased the time of maximum mannitol production from fructose (300 g/L) from 136 to 92 h. One-third of fructose could be replaced with glucose, maltose, galactose, mannose, raffinose, or starch with glucoamylase (simultaneous saccharification and fermentation), and two-thirds of fructose could be replaced with sucrose. L. intermedius B-3693 did not co-utilize lactose, cellobiose, glycerol, or xylose with fructose. It produced lactic acid and ethanol but no acetic acid from glucose. The bacterium produced 21.3 +/- 0.6 g lactic acid, 10.5 +/- 0.3 g acetic acid, and 4.7 +/- 0.0 g ethanol per L of fermentation broth from dilute acid (15% solids, 0.5% H(2)SO(4), 121 degrees C, 1 h) pretreated enzyme (cellulase, beta-glucosidase) saccharified corn fiber hydrolyzate.
Saha
Badal C
BC
Fermentation Biotechnology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture, Peoria, Illinois 61604, USA. sahabc@ncaur.usda.gov
Nakamura
Lawrence K
LK
D019342:Acetic Acid / Q000378:metabolism; D019149:Bioreactors / Q000382:microbiology*; D005632:Fructose / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D019344:Lactic Acid / Q000096:biosynthesis; D007778:Lactobacillus / Q000145:classification* / Q000254:growth & development* / Q000378:metabolism*; D008353:Mannitol / Q000378:metabolism*; D018520:Plant Shoots / Q000737:chemistry; D013045:Species Specificity; D003313:Zea mays / Q000737:chemistry
D005632:Fructose; D019344:Lactic Acid; D008353:Mannitol; D005947:Glucose; D019342:Acetic Acid