Cloning and expression of a novel human C5orf12 gene*, a member of the TMS_TDE family.
We report here cloning and characterization of a novel human gene, termed C5orf12, which is a putative membrane protein belonging to the TMS_TDE family. The cDNA encodes 423 amino acid with a putative molecular weight of about 47 KDa. Secondary structure prediction showed that C5orf12 contained 10 putative transmembrane helices, which has high identity with other family members. We performed RT-PCR to examine its expression pattern. The result showed that C5orf12 was highly expressed in placenta, skeletal muscle, spleen, thymus, testis and peripheral leukocyte while expressed weakly in heart and liver. C5orf12 has high identity with the rat TPO1, so we speculate that C5orf12 may also have a role in the brain development.
Xu
Jian
J
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, People's Republic of China.
Ji
Chaoneng
C
Wang
Liu
L
Cao
Yueqiong
Y
Dai
Jianliang
J
Ye
Xin
X
Zeng
Li
L
Dai
Jianfeng
J
Wu
Qihan
Q
Xie
Yi
Y
Mao
Yumin
Y
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D006801:Humans; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein
D008565:Membrane Proteins; C108500:Serinc5 protein, rat