title: Aspergillus oryzae in solid-state and submerged fermentations. Progress report on a multi-disciplinary project.
abstract: We report the progress of a multi-disciplinary research project on solid-state fermentation (SSF) of the filamentous fungus Aspergillus oryzae. The molecular and physiological aspects of the fungus in submerged fermentation (SmF) and SSF are compared and we observe a number of differences correlated with the different growth conditions. First, the aerial hyphae which occur only in SSFs are mainly responsible for oxygen uptake. Second, SSF is characterised by gradients in temperature, water activity and nutrient concentration, and inside the hyphae different polyols are accumulating. Third, pelleted growth in SmF and mycelial growth in SSF show different gene expression and protein secretion patterns. With this approach we aim to expand our knowledge of mechanisms of fungal growth on solid substrates and to exploit the biotechnological applications.
authors.affiliation: Wageningen Centre for Food Sciences, P.O. Box 557, The Netherlands.
authors.forename: Rob
authors.initials: R
authors.lastname: te Biesebeke
authors.forename: George
authors.initials: G
authors.lastname: Ruijter
authors.forename: Yovita S P
authors.initials: YS
authors.lastname: Rahardjo
authors.forename: Marisca J
authors.initials: MJ
authors.lastname: Hoogschagen
authors.forename: Margreet
authors.initials: M
authors.lastname: Heerikhuisen
authors.forename: Ana
authors.initials: A
authors.lastname: Levin
authors.forename: Kenneth G A
authors.initials: KG
authors.lastname: van Driel
authors.forename: Maarten A I
authors.initials: MA
authors.lastname: Schutyser
authors.forename: Jan
authors.initials: J
authors.lastname: Dijksterhuis
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhu
authors.forename: Frans J
authors.initials: FJ
authors.lastname: Weber
authors.forename: Willem M
authors.initials: WM
authors.lastname: de Vos
authors.forename: Kees A M J J
authors.initials: KA
authors.lastname: van den Hondel
authors.forename: Arjen
authors.initials: A
authors.lastname: Rinzema
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Punt
mesh_terms: D001236:Aspergillus oryzae / Q000235:genetics / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods; D005285:Fermentation / Q000502:physiology; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal / Q000502:physiology; D025301:Hyphae / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption
chemical_list: D010100:Oxygen