title: [Molecular diagnosis of infections].
abstract: Molecular diagnostics (detection of nucleic acids by molecular genetics techniques) become more valuable in clinical diagnosis of disease. Apart from the already long-time used genetic techniques for detection of congenital anomalies, current use of molecular techniques includes detection of microbial pathogens. The character of these techniques increases the possibility of achieving diagnosis in cases where classical cultivation is not possible, is not reliable or is not fast enough. As with every new approach, molecular diagnostics have faced encountered reactions from the scientific community. Some scientists tend to overestimate the value of molecular diagnostic techniques, while sceptics, sometimes influenced by a biased or incomplete knowledge of the technology, think it is of little value. In this work, on the basis of literature and our own data from more than 5 years of experience with these methods, we have assessed the pros and cons of the use of molecular diagnostics of infectious diseases in the light of their potential use in clinical practice.
authors.affiliation: Centrum kardiovaskulární a transplantaèni chirurgie, Brno. jasben@cktch.cz
authors.forename: J
authors.initials: J
authors.lastname: Benedík
authors.forename: J
authors.initials: J
authors.lastname: Cerný
authors.forename: M
authors.initials: M
authors.lastname: Votava
authors.forename: J
authors.initials: J
authors.lastname: Wechsler
authors.forename: R
authors.initials: R
authors.lastname: Horváth
authors.forename: M
authors.initials: M
authors.lastname: Dendis
authors.forename: M
authors.initials: M
authors.lastname: Grijalva
mesh_terms: D001424:Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D025202:Molecular Diagnostic Techniques; D012680:Sensitivity and Specificity; D014777:Virus Diseases / Q000175:diagnosis* / Q000821:virology
vernacular_title: Molekulární diagnostika infekcních stavů.