Expression of estrogen receptor beta exon-deleted variant mRNAs in ovary and uterine endometrium.
Various estrogen receptor beta exon-deleted variant (ER-beta EDV) mRNAs were expressed in human ovary and uterine endometrium. Estrogen receptor beta (ER-beta) completely or partially deleted exon n is expressed as ER-beta EnDV or En'DV, respectively. The mRNAs for ER-beta single exon-deleted variant (EDV), ER-beta E2DV, E4DV, E5DV and E6DV; for ER-beta double exon-deleted variants, ER-beta E1'+2DV, E4+5DV and E5+6DV; and for ER-beta triple exon-deleted variants, ER-beta E2'+3+4DV and E4+5+6DV were detected. In ER-beta E2DV, E4+5DV, E5DV and E6DV mRNAs, the new stop codon is made in the exon following the deleted exon(s), and the new proteins may lack the corresponding domains. In ER-beta E1'+2DV, E2'+3+4DV, E4DV, E4+5+6DV and E5+6DV mRNAs, the original stop codon is still present, and the new proteins may conserve the new short amino acid sequences surrounding the deleted exons. ER-beta E1'+2DV, E2DV, E2'+3+4DV, E4DV, E4+5DV and E4+5+6 are unlikely to work as a transcription factors. On the other hand, ER-beta E5DV, E6DV and E5+6DV lack only the ligand-binding domain, and might work as dominant positive or negative factors. Therefore, ER-beta E5DV, E6DV and E5+6DV, constitutively expressed in human ovary and uterine endometrium might, in part regulate estrogen responsiveness.
Fujimoto
Jiro
J
Department of Obstetrics and Gynecology, Gifu University School of Medicine, 40 Tsukasa-machi, Gifu City 500-8705, Japan. jf@cc.gifu-u.ac.jp
Sun
Wen-Shu
WS
Misao
Ryou
R
Sakaguchi
Hideki
H
Aoki
Ikumi
I
Toyoki
Hiroshi
H
Tamaya
Teruhiko
T
D000328:Adult; D000595:Amino Acid Sequence; D018388:Codon, Terminator; D004717:Endometrium / Q000378:metabolism*; D047629:Estrogen Receptor beta; D005091:Exons; D005260:Female; D006801:Humans; D008024:Ligands; D008875:Middle Aged; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010053:Ovary / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism; D011960:Receptors, Estrogen / Q000096:biosynthesis* / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D018388:Codon, Terminator; D047629:Estrogen Receptor beta; D008024:Ligands; D012333:RNA, Messenger; D011960:Receptors, Estrogen