Immobilization of invertase in conducting copolymers of 3-methylthienyl methacrylate.
Immobilization of invertase in conducting copolymer matrices of 3-methylthienyl methacrylate with pyrrole and thiophene was achieved by constant potential electrolysis using sodium dodecyl sulfate (SDS) as the supporting electrolyte. Polythiophene (PTh) was also used in entrapment process for comparison. Kinetic parameters, Michaelis-Menten constant, K(m), and the maximum reaction rate, V(max), were investigated. Operational stability and temperature optimization of the enzyme electrodes were also examined.
Cirpan
A
A
Department of Chemistry, Middle East Technical University, Ankara, Turkey.
Alkan
S
S
Toppare
L
L
Hepuzer
Y
Y
Yagci
Y
Y
D004563:Electrochemistry; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013499:Surface Properties; D013696:Temperature; D013876:Thiophenes / Q000737:chemistry*; D043324:beta-Fructofuranosidase / Q000737:chemistry* / Q000378:metabolism
D004800:Enzymes, Immobilized; D011758:Pyrroles; D013876:Thiophenes; C481081:poly(3-methylthienyl methacrylate); D012967:Sodium Dodecyl Sulfate; D019904:Polymethyl Methacrylate; D043324:beta-Fructofuranosidase