title: Ion channel gene expression in human lung, skin, and cord blood-derived mast cells.
abstract: Immunoglobulin E (IgE)-dependent activation of human mast cells (HMC) is characterized by an influx of extracellular calcium (Ca(2+)), which is essential for subsequent release of preformed (granule-derived) mediators and newly generated autacoids and cytokines. In addition, flow of ions such as K(+) and Cl(-) is likely to play an important role in mast cell activation, proliferation, and chemotaxis through their effect on membrane potential and thus Ca(2+) influx. It is therefore important to identify these critical molecular effectors of HMC function. In this study, we have used high-density oligonucleotide probe arrays to characterize for the first time the profile of ion channel gene expression in human lung, skin, and cord blood-derived mast cells. These cells express mRNA for inwardly rectifying and Ca(2+)-activated K(+) channels, voltage-dependent Na(+) and Ca(2+) channels, purinergic P2X channels, transient receptor potential channels, and voltage-dependent and intracellular Cl(-) channels. IgE-dependent activation had little effect on ion channel expression, but distinct differences for some channels were observed between the different mast cell phenotypes, which may contribute to the mechanism of functional mast cell heterogeneity.
authors.affiliation: Division of Respiratory Medicine, Institute for Lung Health, University of Leicester Medical School, United Kingdom. pbradding@hotmail.com
authors.forename: Peter
authors.initials: P
authors.lastname: Bradding
authors.forename: Yoshimichi
authors.initials: Y
authors.lastname: Okayama
authors.forename: Naotomo
authors.initials: N
authors.lastname: Kambe
authors.forename: Hirohisa
authors.initials: H
authors.lastname: Saito
mesh_terms: D000328:Adult; D018952:Antigens, CD34 / Q000032:analysis; D015220:Calcium Channels / Q000096:biosynthesis / Q000097:blood / Q000235:genetics; D002478:Cells, Cultured / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D007231:Infant, Newborn; D007473:Ion Channels / Q000096:biosynthesis* / Q000235:genetics; D008168:Lung / Q000166:cytology*; D008407:Mast Cells / Q000145:classification / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D015221:Potassium Channels / Q000096:biosynthesis / Q000145:classification / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D018048:Receptors, Purinergic P2 / Q000096:biosynthesis / Q000235:genetics; D012867:Skin / Q000166:cytology*; D015222:Sodium Channels / Q000096:biosynthesis / Q000145:classification / Q000235:genetics; D050052:TRPC Cation Channels
chemical_list: D018952:Antigens, CD34; D015220:Calcium Channels; D007473:Ion Channels; D015221:Potassium Channels; D012333:RNA, Messenger; D018048:Receptors, Purinergic P2; D015222:Sodium Channels; D050052:TRPC Cation Channels; D007073:Immunoglobulin E; D003907:Dexamethasone