Enantioseparation using cyclosophoraoses as a novel chiral additive in capillary electrophoresis.
Cyclosophoraoses, cyclic beta-(1-->2)-D-glucans produced by Rhizobium meliloti 2011, were used as a novel chiral additive for the separation of terbutaline, amethopterin, thyroxine and N-acetylphenylalanine enantiomers in aqueous capillary electrophoresis (CE). Enantioseparation took place in the normal- or reversed-polarity mode when a high concentration of neutral (60 mM) or anionic (40 mM) cyclosophoraoses was added to the background electrolyte (BGE).
Lee
Sanghoo
S
Department of Microbial Engineering and Bio/Molecular Informatics Center, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, South Korea.
Jung
Seunho
S
D019075:Electrophoresis, Capillary / Q000379:methods*; D005936:Glucans / Q000737:chemistry*; D008727:Methotrexate / Q000737:chemistry; D015394:Molecular Structure; D010649:Phenylalanine / Q000031:analogs & derivatives* / Q000737:chemistry; D012231:Rhizobium / Q000737:chemistry; D013237:Stereoisomerism; D013726:Terbutaline / Q000737:chemistry; D013974:Thyroxine / Q000737:chemistry
D005936:Glucans; D010649:Phenylalanine; D013726:Terbutaline; C044228:N-acetylphenylalanine; D013974:Thyroxine; D008727:Methotrexate