title: Phospholipase D1 is required for efficient mating projection formation in Saccharomyces cerevisiae.
abstract: Phospholipase D1 (PLD1) is an important enzyme involved in lipid signal transduction in eukaryotes. A role for PLD1 in signaling in Saccharomyces cerevisiae was examined. Pheromone response in yeast is controlled by a well-characterized protein kinase cascade. Loss of PLD1 activity was found to impair pheromone-induced changes in cellular morphology that result in formation of mating projections. The rate at which projections appeared following pheromone treatment was delayed, suggesting that PLD1 facilitates the execution of a rate-limiting step in morphogenesis. Mutants were found to be less sensitive to pheromone, again arguing that PLD1 is acting at a rate-limiting step. The fact that morphogenesis is most dramatically affected indicates that PLD1 functions primarily in the morphogenic branch of the pheromone response pathway.
authors.affiliation: Molecular, Cellular Biology and Pathobiology Program, Medical University of South Carolina, Charleston 29425, USA.
authors.forename: M L
authors.initials: ML
authors.lastname: Hairfield
authors.forename: A B
authors.initials: AB
authors.lastname: Ayers
authors.forename: J W
authors.initials: JW
authors.lastname: Dolan
mesh_terms: D003470:Culture Media; D005656:Fungal Proteins / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D009024:Morphogenesis; D010675:Pheromones / Q000378:metabolism*; D010739:Phospholipase D / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins; D015398:Signal Transduction; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D010675:Pheromones; C489241:STE12 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D010739:Phospholipase D; C106801:phospholipase D1