Degradation of phenanthrene and naphthalene by a Burkholderia species strain.
Burkholderia sp. TNFYE-5 was isolated from soil for the ability to grow on phenanthrene as sole carbon and energy source. Unlike most other phenanthrene-degrading bacteria, TNFYE-5 was unable to grow on naphthalene. Growth substrate range experiments coupled with the ring-cleavage enzyme assay data suggest that TNFYE-5 initially metabolizes phenanthrene to 1-hydroxy-2-naphthoate with subsequent degradation through the phthalate and protocatechuate and beta-ketoadipate pathway. A metabolite in the degradation of naphthalene by TNFYE-5 was isolated by high-pressure liquid chromatography (HPLC) and was identified as salicylate by UV-visible spectral and gas chromatography-mass spectrometry analyses. Thus, the inability to degrade salicylate is apparently one major reason for the incapability of TNFYE-5 to grow on naphthalene.
Kang
H
H
Department of Biology and Institute of Life Science, Yonsei University, Seoul 120-749, Korea.
Hwang
S Y
SY
Kim
Y M
YM
Kim
E
E
Kim
Y S
YS
Kim
S K
SK
Kim
S W
SW
Cerniglia
C E
CE
Shuttleworth
K L
KL
Zylstra
G J
GJ
D001673:Biodegradation, Environmental; D019117:Burkholderia / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D004787:Environmental Pollution / Q000032:analysis; D008958:Models, Molecular; D009281:Naphthalenes / Q000378:metabolism*; D010616:Phenanthrenes / Q000378:metabolism*; D010795:Phthalic Acids / Q000378:metabolism; D012988:Soil Microbiology
D009281:Naphthalenes; D010616:Phenanthrenes; D010795:Phthalic Acids; C031721:naphthalene; C031181:phenanthrene; C032279:phthalic acid