title: Nucleotide sequence analysis of the glycoprotein gene of putative spring viraemia of carp virus and pike fry rhabdovirus isolates reveals four genogroups.
abstract: RT-PCR methods have been applied to the detection and sequencing of the glycoprotein gene of putative spring viraemia of carp viruses (SVCV) and pike fry rhabdoviruses (PFRV), including isolates from tench, grass carp, roach, bream and false harlequin, sheatfish and orfe. Phylogenetic analysis of a 550 nucleotide (nt) region of the glycoprotein gene identified 4 groups, I to IV. Significantly, the majority of viruses previously identified as PFRV formed a distinct cluster (Genogroup IV) which shared <80% nucleotide identity with the PFRV reference strain (Genogroup III). The similarity between another PFRV-like virus isolated from grass carp and representatives of Genogroups III and IV was also <80%, indicating that this virus belonged to a third group (Genogroup II). All of the putative SVC viruses were assigned to a 4th group (Genogroup I), sharing <61% nucleotide identity with viruses in Genogroups II to IV.
authors.affiliation: The Centre for the Environment, Fisheries and Aquaculture Science, Weymouth Laboratory, Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, United Kingdom. d.m.stone@cefas.co.uk
authors.forename: D M
authors.initials: DM
authors.lastname: Stone
authors.forename: W
authors.initials: W
authors.lastname: Ahne
authors.forename: K L
authors.initials: KL
authors.lastname: Denham
authors.forename: P F
authors.initials: PF
authors.lastname: Dixon
authors.forename: C T Y
authors.initials: CT
authors.lastname: Liu
authors.forename: A M
authors.initials: AM
authors.lastname: Sheppard
authors.forename: G R
authors.initials: GR
authors.lastname: Taylor
authors.forename: K
authors.initials: K
authors.lastname: Way
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000821:virology; D017859:Esocidae / Q000821:virology; D005393:Fish Diseases / Q000821:virology*; D005838:Genotype; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012209:Rhabdoviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D018353:Rhabdoviridae Infections / Q000662:veterinary* / Q000821:virology; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
chemical_list: D006023:Glycoproteins