Effect of air-powder system on titanium surface on fibroblast adhesion and morphology.
OBJECTIVE
To evaluate the number and morphology of fibroblasts grown on machined titanium healing abutments treated with an airpowder system.


METHODS
Twenty-six abutments were assigned to two experimental groups: control (no treatment) and treated (exposed to the Prophy-Jet for 30 seconds). The specimens were incubated for 24 hours with fibroblastic cells in multiwell plates, followed by routine laboratory processing for scanning electron microscope analysis. The specimens were photographed at x 350, and the cell number was counted on an area of approximately 200 um2.


RESULTS
No significant differences were found on morphology between the groups (P > 0.05); however, the control group presented a significantly greater amount of cells (71.44 +/- 31.93, mean +/- SD) in comparison with treated group (35.31 +/- 28.14), as indicated by a nonpaired t test (P = 0.001).


CONCLUSIONS
The use of an air-abrasive prophylaxis system on the surface of titanium healing abutments reduced the cells proliferation but did not influence cell morphology.
Shibli
Jamil Awad
JA
Department of Periodontology, Dental School at Araraquara, State University of Sao Paulo (UNESP), Araraquara, Sao Paulo, Brazil.
Silverio
Karina Gonzales
KG
Martins
Marilia Compagnoni
MC
Marcantonio júnior
Elcio
E
Rossa júnior
Carlos
C
D000388:Air; D002448:Cell Adhesion; D002452:Cell Count; D018929:Cell Culture Techniques; D048429:Cell Size; D000044:Dental Abutments; D003764:Dental Materials / Q000737:chemistry*; D003777:Dental Prophylaxis / Q000295:instrumentation*; D005347:Fibroblasts / Q000166:cytology*; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D011208:Powders; D018709:Statistics, Nonparametric; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
D003764:Dental Materials; D011208:Powders; D014025:Titanium