Effect of the adenosine A1 receptor agonist GR79236 on trigeminal nociception with blink reflex recordings in healthy human subjects.
GR79236 is a highly potent and selective adenosine A1 receptor agonist that has analgesic and anti-inflammatory actions in humans and animals. In animal models it inhibits trigeminal nerve firing and calcitonin gene-related peptide release which play a pivotal role in migraine pathophysiology. Thus GR79236 may have therapeutic potential in migraine. Although there are no validated human models of migraine, the trigeminal nociceptive pathways may be studied with a novel electrode to elicit nociception-specific blink reflex responses. Twelve healthy female volunteers were randomized in a double-blind, placebo-controlled, cross-over trial to investigate the effect of GR79236 on trigeminal nociceptive pathways, as measured by the blink reflex. A secondary objective was to compare the use of two types of electrode, the standard (SE) and nociception-specific electrodes (NE), to investigate human trigeminal pharmacology. Blink reflexes were elicited with SE and NE before and 30 min after GR79236 (10 microg/kg i.v.) or placebo. The median area under the curve of repeated sweeps of the R2 component of the blink reflex was analysed using analysis of covariance with baseline as covariate. Using NE, GR79236 produced a non-significant reduction of the ipsilateral R2 compared with placebo (P = 0.097) and a significant reduction contralaterally (P = 0.008). No significant changes were observed using SE. There were no significant adverse events. The results suggest that NE is more sensitive than SE to detect pharmacological effects in the trigeminal nociceptive system. Furthermore, the adenosine A1 receptor agonist GR79236 inhibits trigeminal nociception in humans. These results support a possible therapeutic role for GR79236 in primary headache disorders.
Giffin
N J
NJ
Headache Group, Institute of Neurology, London, UK.
Kowacs
F
F
Libri
V
V
Williams
P
P
Goadsby
P J
PJ
Kaube
H
H
D000241:Adenosine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000328:Adult; D001767:Blinking / Q000187:drug effects*; D018592:Cross-Over Studies; D004311:Double-Blind Method; D004558:Electric Stimulation; D004566:Electrodes; D005260:Female; D006801:Humans; D000960:Hypolipidemic Agents / Q000494:pharmacology*; D010147:Pain Measurement / Q000379:methods; D058906:Purinergic P1 Receptor Agonists; D014276:Trigeminal Nerve / Q000187:drug effects*
D000960:Hypolipidemic Agents; D058906:Purinergic P1 Receptor Agonists; D000241:Adenosine; C082299:N-((1S,trans)-2-hydroxycyclopentyl)adenosine