title: Differences in electrophysiological and contractile properties of mammalian cardiac tissues bathed in bicarbonate - and HEPES-buffered solutions.
abstract: OBJECTIVE
  The aim of this study was to compare the action potential configuration, contractility, intracellular Ca2+ and H+ concentrations in mammalian cardiac tissues bathed with Krebs and Tyrode solutions at 37 degrees C.
  
  
  RESULTS
  In Langendorff-perfused guinea-pig hearts, loaded with the fluorescent Ca2+-indicator Fura-2, or H+-sensitive dye carboxy-SNARF, shifts from Krebs to Tyrode solution caused intra-cellular acidification, increased diastolic pressure and [Ca2+]i, decreased systolic pressure and [Ca2+]i, leading to a reduction in the amplitude of [Ca2+]i transients and pulse pressure. Contractility was also depressed in canine ventricular trabeculae when transferred from Krebs to Tyrode solution. Shifts from Krebs to Tyrode solution increased the duration of action potentials in multicellular cardiac preparations excised from canine and rabbit hearts but not in isolated cardiomyocytes. All these changes in action potential morphology, contractility, [Ca2+]i and [H+]i were readily reversible by addition of 26 mmol L(-1) bicarbonate to Tyrode solution. Effects of dofetilide and CsCl, both blockers of the delayed rectifier K current, on action potential duration were compared in Krebs and Tyrode solutions. Dofetilide lengthened rabbit ventricular action potentials in a significantly greater extent in Tyrode than in Krebs solution. Exposure of canine Purkinje fibres to CsCl evoked early after depolarizations within 40 min in all preparations incubated with Tyrode solution, but not in those bathed with Krebs solution.
  
  
  CONCLUSIONS
  It is concluded that the marked differences in action potential morphology, [Ca2+]i, [H+]i and contractility observed between preparations bathed with Krebs and Tyrode solutions are more likely attributable to differences in the intracellular buffering capacities of the two media.
authors.affiliation: Department of Physiology, University of Debrecen, H-4012 Debrecen, Hungary.
authors.forename: L
authors.initials: L
authors.lastname: Fülöp
authors.forename: G
authors.initials: G
authors.lastname: Szigeti
authors.forename: J
authors.initials: J
authors.lastname: Magyar
authors.forename: N
authors.initials: N
authors.lastname: Szentandrássy
authors.forename: T
authors.initials: T
authors.lastname: Ivanics
authors.forename: Z
authors.initials: Z
authors.lastname: Miklós
authors.forename: L
authors.initials: L
authors.lastname: Ligeti
authors.forename: A
authors.initials: A
authors.lastname: Kovács
authors.forename: G
authors.initials: G
authors.lastname: Szénási
authors.forename: L
authors.initials: L
authors.lastname: Csernoch
authors.forename: P P
authors.initials: PP
authors.lastname: Nánási
authors.forename: T
authors.initials: T
authors.lastname: Bányász
mesh_terms: D000200:Action Potentials / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D001639:Bicarbonates / Q000494:pharmacology; D001794:Blood Pressure / Q000187:drug effects; D002118:Calcium / Q000032:analysis*; D002586:Cesium / Q000494:pharmacology; D002712:Chlorides / Q000494:pharmacology; D004285:Dogs; D006168:Guinea Pigs; D006531:HEPES / Q000494:pharmacology; D006321:Heart / Q000187:drug effects / Q000502:physiology*; D006352:Heart Ventricles / Q000187:drug effects; D008297:Male; D009200:Myocardial Contraction / Q000187:drug effects / Q000502:physiology*; D010210:Papillary Muscles / Q000187:drug effects / Q000502:physiology; D010627:Phenethylamines / Q000494:pharmacology; D026902:Potassium Channel Blockers / Q000494:pharmacology; D011522:Protons; D011690:Purkinje Fibers / Q000187:drug effects / Q000502:physiology; D011817:Rabbits; D013449:Sulfonamides / Q000494:pharmacology; D016276:Ventricular Function
chemical_list: D001639:Bicarbonates; D002712:Chlorides; D010627:Phenethylamines; D026902:Potassium Channel Blockers; D011522:Protons; D013449:Sulfonamides; D002586:Cesium; C028019:cesium chloride; C063533:dofetilide; D006531:HEPES; D002118:Calcium