title: [PXD gene controls synthesis of the three anionic peroxidase isoforms in Arabidopsis thaliana].
abstract: The pxd mutant of Arabidopsis thaliana had a changed spectrum of anionic peroxidases: three anionic isoforms in the pxd mutant plant had the same enzymatic activity but relatively high electrophoretic mobility as compared to the analogous isoforms in the wild type plants. These isoforms were the most active anionic peroxidases and could be found in most plant organs. Genetic analysis showed that all three isoforms were controlled by the PXD gene. The activity of one isoform was affected by indolyl-3-acetic acid and other stress factors. Expressed sequence tags (EST) analysis of all putative peroxidase genes of A. thaliana revealed the group of the most actively and nonspecifically expressed genes. The promoter sequences of these genes were screened to find the cis-elements. We propose that the PXD gene encodes one of the nonspecific anionic peroxidases or a protein involved in posttranscriptional or posttranslational modification of the peroxidases.
authors.affiliation: Biological Faculty, Moscow State University, Vorob'evy gory, Moscow, 119899 Russia.
authors.forename: O V
authors.initials: OV
authors.lastname: Lebedeva
authors.forename: T A
authors.initials: TA
authors.lastname: Ezhova
authors.forename: S M
authors.initials: SM
authors.lastname: Musin
authors.forename: N L
authors.initials: NL
authors.lastname: Radiukina
authors.forename: S V
authors.initials: SV
authors.lastname: Shestakov
mesh_terms: D000838:Anions; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D020224:Expressed Sequence Tags; D018506:Gene Expression Regulation, Plant; D007210:Indoleacetic Acids / Q000494:pharmacology; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D009195:Peroxidase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010937:Plant Growth Regulators / Q000494:pharmacology; D018520:Plant Shoots / Q000201:enzymology; D011401:Promoter Regions, Genetic
chemical_list: D000838:Anions; D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; D007527:Isoenzymes; D010937:Plant Growth Regulators; C030737:indoleacetic acid; D009195:Peroxidase
vernacular_title: Gen PXD kontroliruet obrazovanie trekh izoform anionnykh peroksidaz Arabidopsis thaliana.