Analysis of a ginger extract by high-performance liquid chromatography coupled to nuclear magnetic resonance spectroscopy using superheated deuterium oxide as the mobile phase.
A methanolic extract of powdered ginger was separated on a Xterra RP 18 column using deuterium oxide as the eluent and a temperature gradient from 50 to 130 degrees C at 4 degrees C/min. On-line and off-line HPLC-NMR analysis yielded spectra for vanillin, dihydroferulic acid, zingerone and ferulic acid. The identification of dihydroferulic acid and zingerone were confirmed by mass spectroscopy.
Saha
Shikha
S
Department of Chemistry, Loughborough University, Loughborough, Leicestershire LE11 3TU, UK.
Smith
Roger M
RM
Lenz
Eva
E
Wilson
Ian D
ID
D000704:Analysis of Variance; D001547:Benzaldehydes / Q000032:analysis / Q000737:chemistry; D002396:Catechols; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D003373:Coumaric Acids / Q000032:analysis / Q000737:chemistry; D017666:Deuterium Oxide; D005233:Fatty Alcohols / Q000032:analysis / Q000737:chemistry; D020939:Zingiber officinale / Q000737:chemistry*; D006358:Hot Temperature; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation / Q000379:methods*; D013058:Mass Spectrometry; D015394:Molecular Structure; D014867:Water
D001547:Benzaldehydes; D002396:Catechols; D003373:Coumaric Acids; D005233:Fatty Alcohols; D014867:Water; C007845:gingerol; C004999:ferulic acid; C100058:vanillin; D017666:Deuterium Oxide