Expression of CD80 on Kupffer cells is enhanced in cadaveric liver transplants.
In experimental animals inhibition of T cell co-stimulation immediately after organ transplantation effectively prevents rejection. We investigated whether the expression of co-stimulatory molecules is enhanced in cadaveric liver transplants, whether their expression is influenced by the transplantation procedure, and whether variation in expression between liver transplants is related to the occurrence of acute rejection. Expression of CD80, CD86 and the macrophage marker CD68 were determined by immunohistochemistry in biopsies from 40 clinical liver transplants obtained at different time-points during the transplantation procedure, and in normal liver tissue obtained from 10 human livers. Expression of CD80 and CD86 on Kupffer cells was graded by comparison with CD68-staining. In a subgroup CD80 and CD86 mRNA was quantified by real-time detection polymerase chain reaction. CD86 was expressed in all liver transplants and normal livers on the majority of Kupffer cells. CD80 was absent or sporadically expressed in normal liver tissue, but in 18 of 40 liver transplants at least one-quarter of Kupffer cells expressed CD80. CD80- and CD86-mRNA and protein expression in liver transplants did not change during the warm ischaemic and reperfusion phases of the transplantation procedure. CD80-expression on Kupffer cells varied strongly between individual donor livers; this variation was, however, not significantly related to the occurrence of acute rejection after transplantation. In conclusion, in nearly half of cold-preserved cadaveric liver transplants an increased proportion of Kupffer cells express CD80 at the time of transplantation in comparison with normal liver tissue. The expression was not further induced by warm ischaemia and reperfusion. However, the observed variation in CD80-expression between liver transplants is not a accurate predictive measure for acute rejection.
Kwekkeboom
J
J
Department of Gastroenterology & Hepatology, Erasmus Medical Center, Rotterdam, The Netherlands. kwekkeboom@mdl.azr.nl
Kuijpers
M A
MA
Bruyneel
B
B
Mancham
S
S
De Baar-Heesakkers
E
E
Ijzermans
J N M
JN
Bouma
G J
GJ
Zondervan
P E
PE
Tilanus
H W
HW
Metselaar
H J
HJ
D000328:Adult; D015703:Antigens, CD / Q000235:genetics; D015214:Antigens, Differentiation, Myelomonocytic / Q000235:genetics; D018122:B7-1 Antigen / Q000235:genetics*; D051940:B7-2 Antigen; D002102:Cadaver; D016022:Case-Control Studies; D005260:Female; D006084:Graft Rejection / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D016130:Immunophenotyping; D007728:Kupffer Cells / Q000276:immunology*; D016031:Liver Transplantation; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis*; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018709:Statistics, Nonparametric; D014181:Transplantation Immunology; D014184:Transplantation, Homologous; D000097382:CD68 Molecule
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; C067980:CD68 antigen, human; D000097382:CD68 Molecule; C497964:CD86 protein, human