Alterations in mRNA expression of ribosomal protein S9 in hydrogen peroxide-treated neurotumor cells and in rat hippocampus after transient ischemia.
This study was designed to isolate new genes related to apoptosis in rat pheochromocytoma (PC12) cells treated with hydrogen peroxide (H2O2), and to characterize the roles of the genes using both in vitro and in vivo models of oxidative injury. cDNA libraries were prepared from H2O2-treated and -untreated PC12 cells, and a ribosomal protein S9 (RPS9) clone was isolated by a differential screening method. Increase of RPS9 expression in both H2O2-treated PC12 and neuroblastoma (Neuro-2A) cells was shown by Northern blot analysis. Viability of the antisense-transfected Neuro-2A (RPS9-AS) cells following H2O2 treatment was significantly reduced in a dose-dependent manner. In an in vivo model of transient forebrain ischemia, an increase in RPS9 expression was prominent by 1 day postischemia in the granule cell layer neurons of the dentate gyrus. Both activation of caspase-3 and significant recovery of viability following pretreatment with cycloheximide were shown in RPS9-AS cells treated with H2O2. These data suggest that RPS9 plays a protective role in oxidative injury of neuronal cells.
Kim
Seong Yun
SY
Department of Pharmacology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Lee
Mun-Yong
MY
Cho
Kyu Cheol
KC
Choi
Yun-Sik
YS
Choi
Jeong-Sun
JS
Sung
Ki-Wug
KW
Kwon
Oh-Joo
OJ
Kim
Ho-Sik
HS
Kim
In-Kyung
IK
Jeong
Seong-Whan
SW
D000310:Adrenal Gland Neoplasms; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D017403:In Situ Hybridization; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D016716:PC12 Cells; D010673:Pheochromocytoma; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics*; D051381:Rats; D000091243:Ribosomal Protein S9; D012269:Ribosomal Proteins / Q000235:genetics*; D014162:Transfection
D012333:RNA, Messenger; D000091243:Ribosomal Protein S9; D012269:Ribosomal Proteins; C000717187:Rps9 protein, rat; D006861:Hydrogen Peroxide