title: Induction of protective immunity in pigs after immunisation with CpG oligodeoxynucleotides formulated in a lipid-based delivery system (Biphasix).
abstract: A large number of studies demonstrated the immunostimulatory effects of CpG oligonucleotides (ODN), particularly in mice. In the present study, we evaluated the ability of lipid-based delivery systems to enhance the adjuvant effect of CpG-ODN and protect against infection in a porcine pleuropneumonia model. Increased levels of OmlA-specific antibody were detected in animals immunised with OmlA and CpG-ODN formulated in the delivery system Biphasix-vaccine targeting adjuvant (VTA), compared to pigs immunised with VTA without CpG-ODN or CpG-ODN alone. In addition, the responses induced by VTA/CpG formulation were similar to those induced by the commercial adjuvant VSA; however, VTA formulations caused significantly less tissue damage than VSA.
authors.affiliation: Veterinary Infectious Disease Organization, University of Saskatchewan, Saskatoon, Canada S7N 5E3.
authors.forename: V L
authors.initials: VL
authors.lastname: Alcón
authors.forename: M
authors.initials: M
authors.lastname: Foldvari
authors.forename: M
authors.initials: M
authors.lastname: Snider
authors.forename: P
authors.initials: P
authors.lastname: Willson
authors.forename: S
authors.initials: S
authors.lastname: Gomis
authors.forename: R
authors.initials: R
authors.lastname: Hecker
authors.forename: L A
authors.initials: LA
authors.lastname: Babiuk
authors.forename: M E
authors.initials: ME
authors.lastname: Baca-Estrada
mesh_terms: D000818:Animals; D001483:Base Sequence; D015226:Dinucleoside Phosphates / Q000276:immunology* / Q000494:pharmacology; D008055:Lipids / Q000276:immunology* / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D009838:Oligodeoxyribonucleotides / Q000276:immunology* / Q000494:pharmacology; D011001:Pleuropneumonia / Q000276:immunology*; D013552:Swine / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology* / Q000494:pharmacology*
chemical_list: D015226:Dinucleoside Phosphates; D008055:Lipids; D009838:Oligodeoxyribonucleotides; D014614:Vaccines, Synthetic; C480625:biphasix-vaccine targeting adjuvant; C015772:CpG dinucleotide