title: The mammalian two-hybrid assay for detection of coactivator-nuclear receptor interactions.
authors.affiliation: Neurogenetics Inc., La Jolla, CA, USA.
authors.forename: Curtis M
authors.initials: CM
authors.lastname: Tyree
authors.forename: Kay
authors.initials: K
authors.lastname: Klausing
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000032:analysis / Q000235:genetics; D002522:Chlorocebus aethiops; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005347:Fibroblasts; D017930:Genes, Reporter; D017405:Herpes Simplex Virus Protein Vmw65 / Q000235:genetics / Q000378:metabolism; D008024:Ligands; D008156:Luciferases / Q000032:analysis / Q000235:genetics; D008322:Mammals; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D000078723:Nuclear Receptor Interacting Protein 1; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D013329:Structure-Activity Relationship; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C052130:GAL4 protein, S cerevisiae; D017405:Herpes Simplex Virus Protein Vmw65; D008024:Ligands; D009687:Nuclear Proteins; D000078723:Nuclear Receptor Interacting Protein 1; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D008156:Luciferases; D015500:Chloramphenicol O-Acetyltransferase