Light scattering as a diagnostic for protein crystal growth--a practical approach.
Static and dynamic light scattering are discussed as particularly useful tools for studying various aspects of protein crystal growth. Specific applications for prenucleation assays as well as for monitoring postnucleation growth processes are presented. Protein-protein interactions determined by light scattering, which serve as a predictor for favorable crystallization conditions as well as for protein solubility behavior, are detailed. Several precautions regarding the practical aspects of light scattering and interpretation of data are also discussed.
Wilson
W William
WW
Department of Chemistry, Mississippi State University, Box 9573, 39762, Mississippi State, MS, USA
D003460:Crystallization; D008956:Models, Chemical; D008962:Models, Theoretical; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D012542:Scattering, Radiation; D012995:Solubility
D011506:Proteins