Determination of amino acid pairs sensitive to variants in human beta-glucocerebrosidase by means of a random approach.
In this data-based theoretical analysis, we use the random approach to analyse the amino acid pairs in human beta-glucocerebrosidase in order to determine which amino acid pairs are more sensitive to 109 variants from missense mutant human glucocerebrosidase. The rationale of this study is based on our hypothesis and findings that the harmful variants are more likely to occur at randomly unpredictable amino acid pairs and the non-harmful variants are more likely to occur at randomly predictable amino acid pairs. This is because we argue that the randomly predictable amino acid pairs should not be deliberately evolved, whereas the randomly unpredictable amino acid pairs should be deliberately evolved with connection of protein function. The results show, for example, that 93.58% of 109 variants occur at randomly unpredictable amino acid pairs, which account for 71.40% of amino acid pairs in glucocerebrosidase, and the chance of occurrence of the variant is about 4.4 times higher in randomly unpredictable amino acid pairs than in predictable pairs. Hence the randomly unpredictable amino acid pairs are more sensitive to variants in human glucocerebrosidase. The results also suggest that human glucocerebrosidase has a natural tendency to variants.
Wu
Guang
G
Laboratoire de Toxicocinétique et Pharmacocinétique, Faculté de Pharmacie, Université de la Méditerranée Aix-Marseille II, Marseille, France. hongguanglishibahao@yahoo.com
Yan
Shaomin
S
D000596:Amino Acids / Q000378:metabolism*; D014644:Genetic Variation; D005962:Glucosylceramidase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D009154:Mutation; D013329:Structure-Activity Relationship
D000596:Amino Acids; D005962:Glucosylceramidase