Rotation in clinostat results in apoptosis of osteoblastic ROS 17/2.8 cells.
Clinostat is an effective, ground-based tool which can be used to verify data from space flight, and to test hypotheses and experimental conditions for eventual space flights. Rotation in clinostat appears to mimic the microgravity environment by nulling the gravitational vector by continuous averaging. In the present study, we exposed osteoblast-like ROS 17/2.8 cells to a vector-averaged gravity environment in a clinostat and found that the cells undergo apoptotic death during the first 24 hr of clino-rotation. We suggest that apoptosis might be one of the mechanisms for reduced bone formation as observed in actual space flights.
Sarkar
D
D
Department of Endocrinology and Metabolism, Nagoya University, Nagoya, Japan.
Nagaya
T
T
Koga
K
K
Kambe
F
F
Nomura
Y
Y
Seo
H
H
D017209:Apoptosis; D002460:Cell Line; D053938:DNA Fragmentation; D006112:Gravitation; D010006:Osteoblasts / Q000502:physiology*; D012399:Rotation; D013314:Stress, Mechanical; D018474:Weightlessness Simulation