title: Degradation of phenanthrene and naphthalene by a Burkholderia species strain.
abstract: Burkholderia sp. TNFYE-5 was isolated from soil for the ability to grow on phenanthrene as sole carbon and energy source. Unlike most other phenanthrene-degrading bacteria, TNFYE-5 was unable to grow on naphthalene. Growth substrate range experiments coupled with the ring-cleavage enzyme assay data suggest that TNFYE-5 initially metabolizes phenanthrene to 1-hydroxy-2-naphthoate with subsequent degradation through the phthalate and protocatechuate and beta-ketoadipate pathway. A metabolite in the degradation of naphthalene by TNFYE-5 was isolated by high-pressure liquid chromatography (HPLC) and was identified as salicylate by UV-visible spectral and gas chromatography-mass spectrometry analyses. Thus, the inability to degrade salicylate is apparently one major reason for the incapability of TNFYE-5 to grow on naphthalene.
authors.affiliation: Department of Biology and Institute of Life Science, Yonsei University, Seoul 120-749, Korea.
authors.forename: H
authors.initials: H
authors.lastname: Kang
authors.forename: S Y
authors.initials: SY
authors.lastname: Hwang
authors.forename: Y M
authors.initials: YM
authors.lastname: Kim
authors.forename: E
authors.initials: E
authors.lastname: Kim
authors.forename: Y S
authors.initials: YS
authors.lastname: Kim
authors.forename: S K
authors.initials: SK
authors.lastname: Kim
authors.forename: S W
authors.initials: SW
authors.lastname: Kim
authors.forename: C E
authors.initials: CE
authors.lastname: Cerniglia
authors.forename: K L
authors.initials: KL
authors.lastname: Shuttleworth
authors.forename: G J
authors.initials: GJ
authors.lastname: Zylstra
mesh_terms: D001673:Biodegradation, Environmental; D019117:Burkholderia / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D004787:Environmental Pollution / Q000032:analysis; D008958:Models, Molecular; D009281:Naphthalenes / Q000378:metabolism*; D010616:Phenanthrenes / Q000378:metabolism*; D010795:Phthalic Acids / Q000378:metabolism; D012988:Soil Microbiology
chemical_list: D009281:Naphthalenes; D010616:Phenanthrenes; D010795:Phthalic Acids; C031721:naphthalene; C031181:phenanthrene; C032279:phthalic acid