Investigation on the interaction of DNA and electroactive ligands using a rapid electrochemical method.
A rapid method for investigation of the interaction of DNA and electroactive ligands based on an electrochemical equation for irreversible processes is presented. The binding constant (K) and the size of binding site (s) are simultaneously obtained from the dependence of the current on the amount of added DNA in voltammetry. A non-intercalative binder (Hoechst 33258) and two DNA-intercalators (mitoxantrone (MXT) and actinomycin D (AMD)) were examined in experiments. It was found that the binding constant of Hoechst 33258, mitoxantrone and actinomycin D, were 2.1 x 10(8), 8.9 x 10(9) and 9.1 x 10(9) cm(3) mol(-1); and the size of their binding sites were 4, 3 and 8, respectively. The study provides a convenient and sensitive approach for estimating affinity parameters and outlining the interaction between DNA and electroactive targeting compounds.
Wang
Sufen
S
Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028, China.
Peng
Tuzhi
T
Yang
Catherine F
CF
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D003609:Dactinomycin / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods*; D005399:Fishes; D007364:Intercalating Agents / Q000737:chemistry*; D008024:Ligands; D046911:Macromolecular Substances; D008297:Male; D008942:Mitoxantrone / Q000737:chemistry*; D008956:Models, Chemical; D011485:Protein Binding; D013094:Spermatozoa / Q000737:chemistry / Q000378:metabolism
D007364:Intercalating Agents; D008024:Ligands; D046911:Macromolecular Substances; D003609:Dactinomycin; D004247:DNA; C427203:calf thymus DNA; D008942:Mitoxantrone