Reversing the inactivation of peroxiredoxins caused by cysteine sulfinic acid formation.
The active-site cysteine of peroxiredoxins is selectively oxidized to cysteine sulfinic acid during catalysis, which leads to inactivation of peroxidase activity. This oxidation was thought to be irreversible. However, by metabolic labeling of mammalian cells with 35S, we show that the sulfinic form of peroxiredoxin I, produced during the exposure of cells to H2O2, is rapidly reduced to the catalytically active thiol form. The mammalian cells' ability to reduce protein sulfinic acid might serve as a mechanism to repair oxidatively damaged proteins or represent a new type of cyclic modification by which the function of various proteins is regulated.
Woo
Hyun Ae
HA
Center for Cell Signaling Research and Division of Molecular Life Sciences, Ewha Womans University, Seoul 120-750, Korea.
Chae
Ho Zoon
HZ
Hwang
Sung Chul
SC
Yang
Kap-Seok
KS
Kang
Sang Won
SW
Kim
Kanghwa
K
Rhee
Sue Goo
SG
D000818:Animals; D002384:Catalysis; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D003545:Cysteine / Q000031:analogs & derivatives* / Q000378:metabolism*; D019281:Dimerization; D006367:HeLa Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism; D051379:Mice; D018377:Neurotransmitter Agents; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000737:chemistry / Q000378:metabolism*; D054464:Peroxiredoxins; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D021241:Spectrometry, Mass, Electrospray Ionization; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013441:Sulfinic Acids / Q000378:metabolism; D014407:Tumor Cells, Cultured
D018377:Neurotransmitter Agents; D011500:Protein Synthesis Inhibitors; D013438:Sulfhydryl Compounds; D013441:Sulfinic Acids; D003513:Cycloheximide; D008715:Methionine; D006861:Hydrogen Peroxide; D010544:Peroxidases; D054464:Peroxiredoxins; D003545:Cysteine; C013461:cysteine sulfinic acid