Laryngeal effects of antigen stimulation challenge with perennial allergen Dermatophagoides pteronyssinus.
OBJECTIVE
We conducted a pilot study to assess the effects of antigen stimulation on the appearance and function of the larynx.


METHODS
The prospective, double-blind, randomized study included 9 adult patients with a skin-prick test positive for Dermatophagoides pteronyssinus.


METHODS
Subjects were blindly challenged via nebulizer with either an active antigenic suspension or placebo. Baseline and 30-minute evaluations of the larynx were performed. Assessments included subjective voice and videostroboscopic assessments, acoustic analysis of voice, speech aerodynamic testing, and allergy and voice handicap questionnaires.


RESULTS
Although both inflammation and increased mucus were noted, there were no significant differences between the antigen- and placebo-exposed subjects on any of the measures obtained.


CONCLUSIONS
Our preliminary investigation was not successful in demonstrating a direct causal relationship between antigen exposure and physical or functional changes in the larynx. Future studies will involve modifications to our current methodology, including increasing the concentration of antigen, prolonging the exposure time, and observing for late phase responses.
Reidy
Patrick M
PM
Otolaryngology-Head and Neck Surgery, Wayne State University, Detroit, Michigan, USA. pmreidy@owa.med.wayne.edu
Dworkin
James P
JP
Krouse
John H
JH
D000328:Adult; D000368:Aged; D039741:Antigens, Dermatophagoides / Q000276:immunology*; D004311:Double-Blind Method; D005260:Female; D006801:Humans; D007830:Larynx / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects; D011446:Prospective Studies; D011795:Surveys and Questionnaires
D039741:Antigens, Dermatophagoides