title: Local intravascular coagulation and fibrin deposition on intestinal ischemia-reperfusion in rats.
abstract: BACKGROUND
  This study investigates intravascular coagulation and thrombotic obstruction in the splanchnic vasculature after intestinal ischemia in relation to epithelial integrity and function.
  
  
  METHODS
  Intestinal ischemia was induced in rats by superior mesenteric artery occlusion for 20 or 40 minutes. Intestinal injury was assessed by histologic analysis, biochemical markers, and functional studies. During reperfusion, portal and systemic blood samples were collected to analyze activation of coagulation and fibrinolysis.
  
  
  RESULTS
  Superior mesenteric artery occlusion resulted in mild to moderate intestinal injury. Twenty and 40 minutes of ischemia and 3 hours of reperfusion resulted in local intestinal thrombin generation and conversion of fibrinogen to fibrin, reflected by 3- and 4-fold increases in thrombin-antithrombin complex levels and a 3-fold elevation of fibrin degradation products (D-dimer), respectively. During reperfusion, after a short-lasting initial activation of local fibrinolysis, plasminogen activator activity was suppressed, as indicated by an approximately 4-fold increase in portal plasma levels of the plasminogen activator inhibitor. D-dimer levels showed that activation of coagulation and depression of fibrinolysis resulted in fibrin formation, which was confirmed to be intravascular fibrin deposition by histologic examination.
  
  
  CONCLUSIONS
  Intestinal ischemia-reperfusion results in local intravascular coagulation and fibrin deposition.
authors.affiliation: Department of Surgery, Surgical Laboratory, IWO-1-155, Academic Medical Center-University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands.
authors.forename: Ivo G
authors.initials: IG
authors.lastname: Schoots
authors.forename: Marcel
authors.initials: M
authors.lastname: Levi
authors.forename: E H Paulina
authors.initials: EH
authors.lastname: Roossink
authors.forename: Pieter B
authors.initials: PB
authors.lastname: Bijlsma
authors.forename: Thomas M
authors.initials: TM
authors.lastname: van Gulik
mesh_terms: D000818:Animals; D001777:Blood Coagulation; D001778:Blood Coagulation Disorders / Q000209:etiology* / Q000378:metabolism / Q000473:pathology*; D005337:Fibrin / Q000378:metabolism*; D005342:Fibrinolysis; D005947:Glucose / Q000493:pharmacokinetics; D007408:Intestinal Absorption; D007422:Intestines / Q000098:blood supply; D008297:Male; D008833:Microcirculation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000150:complications* / Q000378:metabolism / Q000473:pathology*; D013152:Splanchnic Circulation; D013927:Thrombosis / Q000378:metabolism; D014867:Water / Q000378:metabolism
chemical_list: D014867:Water; D005337:Fibrin; D005947:Glucose