title: Identification of an allele of CLA1 associated with variegation in Arabidopsis thaliana.
abstract: We have identified a mutant of Arabidopsis thaliana (lvr111) that exhibits a variegated phenotype, reduced isoprenoid pigmentation, and dwarfism in comparison with wild-type plants. Segregation analysis indicated that this phenotype was caused by a single, semi-dominant mutation and PCR-based marker mapping placed the mutation near position 56 on the RI map of chromosome IV. The lvr111 lesion was identified by genomic PCR and sequence analysis as a missense mutation (D306N) in the CLA1 gene (AT4g15560) and complementation analysis confirmed the allelic relationship between lvr111 and CLA1. CLA1 encodes 1-deoxy-d-xylulose 5-phosphate synthase, which catalyses the first step of the non-mevalonate isoprenoid biosynthetic pathway. These observations demonstrate that, unlike the albinism caused by severe alleles of CLA1, weaker alleles are associated with leaf variegation.
authors.affiliation: Department of Biology, Indiana University-Purdue University Indianapolis, Indianapolis, IN 46202, USA Department of Chemistry, State University of New York - ESF, Syracuse, NY 13210, USA Present address: Department of Horticulture, University of Wisconsin-Madison, Madison, WI 53706, USA Present address: Department of Botany and Plant Sciences, University of California Riverside, Riverside, CA 92521, USA.
authors.forename: Dring N.
authors.initials: DN
authors.lastname: Crowell
authors.forename: Clay E.
authors.initials: CE
authors.lastname: Packard
authors.forename: Charles A.
authors.initials: CA
authors.lastname: Pierson
authors.forename: José-Luis
authors.initials: JL
authors.lastname: Giner
authors.forename: Brian P.
authors.initials: BP
authors.lastname: Downes
authors.forename: S. Narasimha
authors.initials: SN
authors.lastname: Chary