Higher degree of chromosome mosaicism in preimplantation embryos from carriers of robertsonian translocation t(13;14) in comparison with embryos from karyotypically normal IVF patients.
OBJECTIVE
To compare the frequency and the degree of mosaicism in human embryos from Robertsonian translocation (RT) t(13;14) carriers, with embryos from karyotypically normal IVF patients


METHODS
FISH analysis of embryos from PGD cycles for RT t(13;14), with probes for chromosomes 13, 14, and 18 (Group I) and of embryos from karyotypically normal IVF patients with probes for chromosomes 13, 18, 21, X, and Y (Group II).


RESULTS
The incidence of abnormal mosaic embryos was significantly higher in group I (38/51) as compared with group II (6/45) (chi2: P < 0.01). Furthermore, in group I the percentage of diploid cells per embryo was lower for chromosome 13 and 14 in comparison with 18, while in group II no differences were observed between the five chromosomes analyzed.


CONCLUSIONS
RT induces a high frequency of mosaicism specifically for the chromosomes implicated in the translocation; the analysis by FISH of two blastomeres is strongly recommended for these patients
Emiliani
Serena
S
Fertility Clinic and Laboratory of Research on Human Reproduction, Erasmus Hospital, Free University of Brussels, Belgium. semilian@ulb.ac.be
Gonzalez-Merino
Eric
E
Van den Bergh
Marc
M
Abramowicz
Marc
M
Englert
Yvon
Y
D000328:Adult; D001755:Blastocyst / Q000502:physiology*; D002869:Chromosome Aberrations / Q000196:embryology*; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics; D002887:Chromosomes, Human, Pair 18 / Q000235:genetics; D002891:Chromosomes, Human, Pair 21 / Q000235:genetics; D041321:Chromosomes, Human, X / Q000235:genetics; D041322:Chromosomes, Human, Y / Q000235:genetics; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D009030:Mosaicism / Q000235:genetics*; D011247:Pregnancy; D019836:Preimplantation Diagnosis; D014178:Translocation, Genetic / Q000235:genetics*