The Hansenula polymorpha PDD7 gene is essential for macropexophagy and microautophagy.
Hansenula polymorpha PDD genes are involved in the selective degradation of peroxisomes via macropexophagy. We have isolated various novel pdd mutants by a gene-tagging method. Here we describe the isolation and characterisation of PDD7, which encodes a protein with high sequence similarity (40% identity) to Saccharomyces cerevisiae Apg1p/Aut3p, previously described to be involved in random autophagy and the cytoplasm-to-vacuole targeting pathway. Our data indicate that HpPdd7p is essential for two processes that degrade peroxisomes, namely the highly selective process of macropexophagy and microautophagy, which occurs in H. polymorpha upon nitrogen starvation.
Komduur
Janet A
JA
Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Kerklaan 30, 9751 NN, Haren, The Netherlands.
Veenhuis
Marten
M
Kiel
Jan A K W
JA
D000595:Amino Acid Sequence; D001343:Autophagy / Q000235:genetics; D001692:Biological Transport; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D016681:Genome, Fungal; D008969:Molecular Sequence Data; D020675:Peroxisomes / Q000378:metabolism; D010843:Pichia / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D017386:Sequence Homology, Amino Acid; D014617:Vacuoles / Q000201:enzymology / Q000648:ultrastructure
D005656:Fungal Proteins