Novel synthetic phytochelatin-based capacitive biosensor for heavy metal ion detection.
A novel capacitance biosensor based on synthetic phytochelatins for sensitive detection of heavy metals is described. Synthetic phytochelatin (Glu-Cys)(20)Gly (EC20) fused to the maltose binding domain protein was expressed in Escherichia coli and purified for construction of the biosensor. The new biosensor was able to detect Hg(2+), Cd(2+), Pb(2+), Cu(2+) and Zn(2+) ions in concentration range of 100 fM-10 mM, and the order of sensitivity was S(Zn)>S(Cu)>S(Hg)>>S(Cd) congruent with S(Pb). The biological sensing element of the sensor could be regenerated using EDTA and the storage stability of the biosensor was 15 days.
Bontidean
Ibolya
I
Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00, Lund, Sweden.
Ahlqvist
Josefin
J
Mulchandani
Ashok
A
Chen
Wilfred
W
Bae
Weon
W
Mehra
Rajesh K
RK
Mortari
Alessia
A
Csöregi
Elisabeth
E
D015374:Biosensing Techniques; D025802:Electric Capacitance; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005978:Glutathione; D008667:Metalloproteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D019216:Metals, Heavy / Q000032:analysis* / Q000737:chemistry*; D008832:Microchemistry / Q000295:instrumentation / Q000379:methods; D054811:Phytochelatins; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D008667:Metalloproteins; D019216:Metals, Heavy; D011993:Recombinant Fusion Proteins; D054811:Phytochelatins; D005978:Glutathione