Interaction of thyroid-stimulating antibody with Graves' thyroid-stimulating hormone-binding antibody.
OBJECTIVE
Evidence of anti-thyroid-stimulating hormone (TSH) antibody in Graves' serum has been reported. We found that extremely high Graves' anti-TSH antibodies neutralized other Graves' thyroid-stimulating antibody (TSAb) activity.


METHODS
TSAb-IgG was affinity-purified by Sepharose-bound Graves' anti-TSH antibody (extremely high).


RESULTS
The thyroid-stimulating activity in affinity-purified TSAb-IgG increased about 4-5 times compared to that before purification. TSH-binding inhibitory immunoglobulin (TBII) activity in affinity-purified TSAb-IgG also increased using TSH receptor-coated tube assay. A similar increase of thyroid-stimulating activity accompanied with TBII activity was also observed in affinity-purified TSAb-IgG-F(ab')(2).


CONCLUSIONS
This suggests the possibility that either TSAb may be an anti-idiotypic antibody against anti-TSH antibody or anti-TSH antibody may be an anti-idiotypic antibody against anti-TSH receptor antibody.
Ochi
Y
Y
Research Institute for Production Development, Shimogamo, Kyoto, Japan.
Kajita
Y
Y
Hamazu
M
M
Nagata
A
A
D000888:Antibodies, Anti-Idiotypic / Q000097:blood / Q000276:immunology; D000915:Antibody Affinity; D001323:Autoantibodies / Q000097:blood / Q000276:immunology*; D006111:Graves Disease / Q000097:blood / Q000276:immunology*; D006801:Humans; D018828:Immunoglobulins, Thyroid-Stimulating / Q000097:blood / Q000276:immunology* / Q000302:isolation & purification; D011989:Receptors, Thyrotropin / Q000097:blood / Q000276:immunology*; D013972:Thyrotropin / Q000097:blood / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D001323:Autoantibodies; D018828:Immunoglobulins, Thyroid-Stimulating; D011989:Receptors, Thyrotropin; C094372:thyrotropin-binding inhibitory immunoglobulin; D013972:Thyrotropin