title: Plasmid DNA encoding human carcinoembryonic antigen (CEA) adsorbed onto cationic microparticles induces protective immunity against colon cancer in CEA-transgenic mice.
abstract: A carcinoembryonic antigen (CEA)-based DNA vaccine, adsorbed onto cationic microparticles of poly(DL-lactide-co-glycolide) (PLG) induced tumor-protective immunity against a lethal challenge of MC38-CEA colon carcinoma cells in CEA-transgenic mice that was more potent than that of the corresponding naked DNA vaccine. Boosting with a plasmid encoding murine GM-CSF increased the vaccine's efficacy leading to a complete rejection of tumor cells in 50% of mice. This effect was due to activation of MHC class I-restricted CD8(+) T cells coupled with an increased secretion of proinflammatory cytokines IFN-gamma, TNF-alpha and IL-2. Also, specific activation of dendritic cells was indicated by a two-three-fold upregulation of their costimulatory CD80 and MHC class II molecules. This approach may be a promising new strategy for the rational design of cancer vaccines for future clinical applications.
authors.affiliation: Department of Immunology, The Scripps Research Institute, 10550 North Torrey Pines Road, IMM13, La Jolla, CA 92037, USA.
authors.forename: Yunping
authors.initials: Y
authors.lastname: Luo
authors.forename: Derek
authors.initials: D
authors.lastname: O'Hagan
authors.forename: He
authors.initials: H
authors.lastname: Zhou
authors.forename: Manmohan
authors.initials: M
authors.lastname: Singh
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Ulmer
authors.forename: Ralph A
authors.initials: RA
authors.lastname: Reisfeld
authors.forename: F
authors.initials: F
authors.lastname: James Primus
authors.forename: Rong
authors.initials: R
authors.lastname: Xiang
mesh_terms: D000230:Adenocarcinoma / Q000276:immunology* / Q000517:prevention & control; D000818:Animals; D002272:Carcinoembryonic Antigen / Q000235:genetics* / Q000276:immunology*; D003110:Colonic Neoplasms / Q000276:immunology* / Q000517:prevention & control; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010957:Plasmids; D014614:Vaccines, Synthetic / Q000276:immunology*
chemical_list: D002272:Carcinoembryonic Antigen; D014614:Vaccines, Synthetic