title: Increased in vitro cytopathicity of CC chemokine receptor 5-restricted human immunodeficiency virus type 1 primary isolates correlates with a progressive clinical course of infection.
abstract: The presence of only non-syncytium-inducing beta-chemokine receptor 5-restricted (R5/NSI) human immunodeficiency virus type 1 (HIV-1) in an infected individual has been associated with long-term asymptomatic survival. However, the majority of R5/NSI HIV-1-infected individuals do progress to AIDS. Here, we compared the replicative capacity and cytopathicity of R5/NSI HIV-1 variants that were isolated early and late in the clinical course from 7 long-term asymptomatic individuals and 7 individuals with progressive HIV-1 infection. R5/NSI HIV-1 cytopathicity in vitro directly correlated with in vitro replication. HIV-1 variants obtained early and late during long-term asymptomatic HIV infection from the same individual were equally cytopathic. In contrast, HIV-1 variants obtained during late-stage progressive HIV infection were more cytopathic than viruses obtained early in infection from the same individuals. Our data indicate that the cytopathicity of HIV-1 variants may increase with progression to disease.
authors.affiliation: Sanquin Research and Landsteiner Laboratory of the Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: David
authors.initials: D
authors.lastname: Kwa
authors.forename: Jose
authors.initials: J
authors.lastname: Vingerhoed
authors.forename: Brigitte
authors.initials: B
authors.lastname: Boeser
authors.forename: Hanneke
authors.initials: H
authors.lastname: Schuitemaker
mesh_terms: D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D018450:Disease Progression; D006678:HIV / Q000472:pathogenicity* / Q000502:physiology*; D015658:HIV Infections / Q000473:pathology / Q000503:physiopathology* / Q000821:virology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000821:virology; D010835:Phytohemagglutinins / Q000494:pharmacology; D019713:Receptors, CCR5 / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D010835:Phytohemagglutinins; D019713:Receptors, CCR5