Discovery of novel transcription control relationships with gene regulatory networks generated from multiple-disruption full genome expression libraries.
Gene regulatory networks elucidated from strategic, genome-wide experimental data can aid in the discovery of novel gene function information and expression regulation events from observation of transcriptional regulation among genes of known and unknown biological function. To create a reliable and comprehensive data set for the elucidation of transcription regulation networks, we conducted systematic genome-wide disruption expression experiments of yeast on 118 genes with known involvement in transcription regulation. We report several novel regulatory relationships between known transcription factors and other genes with previously unknown biological function discovered with this expression library. Here we report the downstream regulatory subnetworks for UME6 and MET28. The elucidated network topology among these genes demonstrates MET28's role as a nodal point between genes involved in cell division and those involved in DNA repair mechanisms.
Aburatani
Sachiyo
S
Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan.
Tashiro
Kosuke
K
Savoie
Christopher J
CJ
Nishizawa
Mayumi
M
Hayashi
Kaori
K
Ito
Yuzuru
Y
Muta
Shigeru
S
Yamamoto
Kiyoshi
K
Ogawa
Miki
M
Enomoto
Akiko
A
Masaki
Momoka
M
Watanabe
Shouji
S
Maki
Yukihiro
Y
Takahashi
Yoriko
Y
Eguchi
Yukihiro
Y
Sakaki
Yoshiyuki
Y
Kuhara
Satoru
S
D000465:Algorithms; D005809:Genes, Regulator; D015698:Genomic Library; D020411:Oligonucleotide Array Sequence Analysis; D014158:Transcription, Genetic