title: Conservation of a Pumilio-Nanos complex from Drosophila germ plasm to human germ cells.
abstract: Germ cells are the cells which ultimately give rise to mature sperm and eggs. In model organisms such as flies and worms, several genes that are required for formation and maintenance of germ cells have been identified and their interactions are rapidly being delineated. By contrast, little is known of the genes required for development of human germ cells and it is not clear whether findings from model organisms will translate into knowledge of human germ cell development, especially given observations that reproductive pathways may evolve more rapidly than somatic pathways. The Pumilio and Nanos genes have been especially well-characterized in model organisms and encode proteins that interact and are required for development of germ stem cells in one or both sexes. Here we report the first characterization of a mammalian Nanos homolog, human NANOS1 ( NOS1). We show that human NOS1 protein interacts with the human PUMILIO-2 (PUM2) protein via highly conserved domains to form a stable complex. We also show that in men, the NOS1 and PUM2 proteins are particularly abundant in germline stem cells. These observations mirror those in distant species and document for the first time a conserved protein-protein interaction in germ cells from flies to humans. These results suggest the possibility that the interaction of PUM2 and NOS1 may play a conserved role in germ cell development and maintenance in humans as in model organisms.
authors.affiliation: Center for Reproductive Sciences, Department of Obstetrics, Gynecology and Reproductive Sciences, University of California at San Francisco, San Francisco, CA 94143-0546, USA.
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Jaruzelska
authors.forename: Maciej
authors.initials: M
authors.lastname: Kotecki
authors.forename: Kamila
authors.initials: K
authors.lastname: Kusz
authors.forename: Anna
authors.initials: A
authors.lastname: Spik
authors.forename: Meri
authors.initials: M
authors.lastname: Firpo
authors.forename: Renee A
authors.initials: RA
authors.lastname: Reijo Pera
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002874:Chromosome Mapping; D029721:Drosophila Proteins / Q000235:genetics*; D019143:Evolution, Molecular; D015870:Gene Expression; D005854:Germ Cells; D006801:Humans; D007150:Immunohistochemistry; D011233:Precipitin Tests; D016601:RNA-Binding Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D029721:Drosophila Proteins; D016601:RNA-Binding Proteins; D014157:Transcription Factors; C074333:pum protein, Drosophila; C465505:pumilio protein, human; C070574:nos protein, Drosophila