title: Solid-phase microextraction of volatile compounds from the chopped leaves of three species of Eucalyptus.
abstract: Headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography and ion-trap mass spectrometry has been used to identify biogenic volatile organic compounds present in the headspace of chopped leaves of Eucalyptus (E.) dunnii, E. citriodora, and E. saligna. A simple HS-SPME method entailing 30 min of extraction at 30 degrees C was developed for this purpose. Thirty compounds were identified in the headspace of 60 juvenile chopped Eucalyptus leaves, and another 30 were tentatively identified. The presence of compounds such as (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT), (E,E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene (TMNT), (E,E)-alpha-farnesene, (E,E,E)-3,7,11,15-tetramethyl-1,3,6,10,14-hexadecapentaene (TMHP), beta-caryophyllene, alpha-humulene, germacrene D, and beta-cubebene in the headspace of the leaves but not in the essential oils from the same Eucalyptus trees and information about the infochemical roles of some of these compounds in other living plant systems suggest they might play a bioactive role in Eucalyptus leaves.
authors.affiliation: Department of Chemistry, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1.
authors.forename: Cláudia A
authors.initials: CA
authors.lastname: Zini
authors.forename: Kelen D
authors.initials: KD
authors.lastname: Zanin
authors.forename: Eva
authors.initials: E
authors.lastname: Christensen
authors.forename: Elina B
authors.initials: EB
authors.lastname: Caramão
authors.forename: Janusz
authors.initials: J
authors.lastname: Pawliszyn
mesh_terms: D005052:Eucalyptus / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D009930:Organic Chemicals / Q000737:chemistry / Q000302:isolation & purification*; D018515:Plant Leaves / Q000737:chemistry; D013045:Species Specificity; D014835:Volatilization
chemical_list: D009930:Organic Chemicals