title: Complexity in natural microbial ecosystems: the Guerrero Negro experience.
abstract: The goal of this project is to describe and understand the organismal composition, structure, and physiology of microbial ecosystems from hypersaline environments. One collection of such ecosystems occurs at North America's largest saltworks, the Exportadora de Sal, in Guerrero Negro, Baja California Sur. There, seawater flows through a series of evaporative basins with an increase in salinity until saturation is reached and halite crystallization begins. Several of these ponds are lined with thick (10 cm) microbial mats that have received some biological study. To determine the nature and extent of diversity of the microbial organisms that constitute these ecosystems, we are conducting a phylogenetic analysis using molecular approaches, based on cloning and sequencing of small subunit (SSU) rRNA genes (16S for Bacteria and Archaea, 18S for Eukarya). In addition, we report preliminary results on the microbial composition of a laminated community that occurs in a crystallized gypsum-halite matrix in near-saturated salt water. Exposure of the interior of these large (kilogram) wet, endoevaporite crystals reveals a multitude of colors: layers of yellow, green, pink, and purple microbiota. To date, analyses of these two environments indicate the ubiquitous dominance of uncultured organisms of phylogenetic kinds not generally thought to be associated with hypersaline environments.
authors.affiliation: Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, 347 UCB, Boulder, Colorado 80309, USA.
authors.forename: John R
authors.initials: JR
authors.lastname: Spear
authors.forename: Ruth E
authors.initials: RE
authors.lastname: Ley
authors.forename: Alicia B
authors.initials: AB
authors.lastname: Berger
authors.forename: Norman R
authors.initials: NR
authors.lastname: Pace
mesh_terms: D001419:Bacteria / Q000235:genetics*; D044822:Biodiversity; D003116:Color; D017931:DNA Primers; D008800:Mexico; D010802:Phylogeny; D012623:Seawater; D012965:Sodium Chloride / Q000737:chemistry
chemical_list: D017931:DNA Primers; D012965:Sodium Chloride