Induction of pilocarpine formation in jaborandi leaves by salicylic acid and methyljasmonate.
Jaborandi seedlings were subjected to different treatments in order to study the induction of pilocarpine in the leaves. In addition four extraction methods were assessed to extract the alkaloid from dried leaves. The highest yielding extraction and recovery was observed when dried leaves were first treated with base and then extracted with chloroform. Salt stress (NaCl), wounding, hypoxia, and N and K omission of the nutrient soln caused reductions in pilocarpine contents. Whereas complete nutrient soln and P omission maintained normal levels of the alkaloid. Salicylic acid and methyljasmonate induced a 4-fold increase of pilocarpine, but this increase was dependent on the concentration and time after exposure.
Avancini
Graziela
G
Departamento de Fisiologia Vegetal, Instituto de Biologia, CP 6109, Universidade Estadual de Campinas, CEP 13083-970, Campinas, SP, Brazil
Abreu
Ilka N
IN
Saldaña
Marleny D A
MD
Mohamed
Rahoma S
RS
Mazzafera
Paulo
P
D000085:Acetates / Q000494:pharmacology*; D003517:Cyclopentanes / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D000860:Hypoxia / Q000378:metabolism; D054883:Oxylipins; D010862:Pilocarpine / Q000096:biosynthesis* / Q000302:isolation & purification; D032103:Pilocarpus / Q000187:drug effects* / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D020156:Salicylic Acid / Q000494:pharmacology*; D012964:Sodium / Q000172:deficiency; D012965:Sodium Chloride / Q000494:pharmacology; D013314:Stress, Mechanical
D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D010862:Pilocarpine; D012965:Sodium Chloride; C072239:methyl jasmonate; D012964:Sodium; D020156:Salicylic Acid; D011188:Potassium