Effect of food on gastrointestinal transit of liquids in cynomolgus monkeys.
We investigated the gastrointestinal transit of liquids, as well as various gastric pH profiles, in fed cynomolgus monkeys. Twelve grams of a biscuit-type solid food were provided 1 h before the test. The acetaminophen method was used to determine the gastric half-emptying time (t(50%)), which provided an estimate of the gastric emptying rate. The gastric emptying rate of liquids was significantly reduced after food intake in monkeys. The mean t(50%) value was 143.5 min and comparable to that of humans after eating. However, there was a large variability in the t(50%) between individual animals as shown by the coefficient of variance of approximately 80%. Next, the median oro-caecal transit time in fed monkeys was determined to be 1.8 h, using the sulfasalazine-sulfapyridine method. There was no significant difference in oro-caecal transit time between unfed and fed monkeys; thus, food intake has no significant effect on the oro-caecal transit time of liquids in either monkeys or humans. However, the oro-caecal transit time in humans is about 2 h longer than that in monkeys. Our experiments using several different foods suggested that the typical human gastric pH profile could not be simulated in fed monkeys.
Kondo
Hiromu
H
Novel Pharmaceutical Laboratories, Institute for Technology Development, Yamanouchi Pharmaceutical Co., Ltd., 180 Ozumi, Yaizu-shi, Shizuoka 425-0072, Japan. kondo@yamanouchi.co.jp
Watanabe
Takashi
T
Yokohama
Shigeharu
S
Watanabe
Jun
J
D000082:Acetaminophen / Q000008:administration & dosage; D000284:Administration, Oral; D000818:Animals; D005502:Food; D005745:Gastric Acidity Determination; D005746:Gastric Emptying; D005772:Gastrointestinal Transit / Q000187:drug effects* / Q000502:physiology*; D006801:Humans; D008252:Macaca fascicularis / Q000502:physiology*; D008297:Male; D023421:Models, Animal; D013427:Sulfapyridine / Q000008:administration & dosage; D012460:Sulfasalazine / Q000008:administration & dosage; D013997:Time Factors
D000082:Acetaminophen; D012460:Sulfasalazine; D013427:Sulfapyridine