Carboxyfluorescein diacetate succinimidyl ester facilitates cell tracing and colocalization studies in bioartificial organ engineering.
BACKGROUND
We demonstrate a method that includes colocalization studies to analyze cell suspensions after isolation and to characterize 3-dimensional grafts consisting of cells and matrix in vitro and in vivo.


METHODS
Neonatal rat cardiomyocytes were labelled by CFDA-SE after harvest. Cells in the isolated cell suspension, the embodied cells in the seeded scaffolds were characterized measuring features such as viability and distribution of the cell types.


RESULTS
Selective cell count revealed high yields of viable cardiomyocytes. After seeding cells in collagen matrix, viability of the cells decreased gradually in the time process in vitro. Histology of implanted bioartificial myocardial tissue detected viable cardiomyocytes within the graft.


CONCLUSIONS
Using colocalization histology we could label and track cells within the bioartificial myocardial tissue graft in vitro and post implant and assess viability and distribution.
Mueller-Stahl
K
K
Leibniz Laboratories for Biotechnology and Artificial Organs, Hannover, Germany. Knut.Mueller-Stahl@gmx.de
Kofidis
T
T
Akhyari
P
P
Wachsmann
B
B
Lenz
A
A
Boublik
J
J
Heine
M
M
Muehlfait
V
V
Haverich
A
A
Mertsching
H
H
D000818:Animals; D000831:Animals, Newborn; D021522:Bioartificial Organs; D002465:Cell Movement / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D005452:Fluoresceins / Q000494:pharmacology*; D005456:Fluorescent Dyes / Q000494:pharmacology*; D006354:Heart, Artificial; D032383:Myocytes, Cardiac / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013388:Succinimides / Q000494:pharmacology*; D023822:Tissue Engineering / Q000379:methods*
C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D005452:Fluoresceins; D005456:Fluorescent Dyes; D013388:Succinimides