GSH2, a gene encoding gamma-glutamylcysteine synthetase in the methylotrophic yeast Hansenula polymorpha.
The GSH2 gene, encoding Hansenula polymorpha gamma-glutamylcysteine synthetase, was cloned by functional complementation of a glutathione (GSH)-deficient gsh2 mutant of H. polymorpha. The gene was isolated as a 4.3-kb XbaI fragment that was capable of restoring GSH synthesis, heavy-metal resistance and cell proliferation when introduced into gsh2 mutant cells. It possesses 53% identical and 69% similar amino acids compared with the Candida albicans homologue (Gcs1p). In comparison to the Saccharomyces cerevisiae homologue (Gsh1p), it possesses 47% identical and 61% similar amino acids. The GSH2 sequence appears in the GenBank database under accession No. AF435121.
Ubiyvovk
Vira M
VM
Institute of Cell Biology, Drahomanov Str. 14/16, Lviv 79005, Ukraine.
Nazarko
Taras Y
TY
Stasyk
Olena G
OG
Sohn
Min Jeong
MJ
Kang
Hyun Ah
HA
Sibirny
Andrei A
AA
D000595:Amino Acid Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D005978:Glutathione / Q000096:biosynthesis / Q000172:deficiency; D005981:Glutathione Synthase / Q000235:genetics* / Q000378:metabolism; D000432:Methanol / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D010843:Pichia / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D016415:Sequence Alignment
D005981:Glutathione Synthase; D005978:Glutathione; D000432:Methanol