Removal of phenanthrene from soil by co-cultures of bacteria and fungi pregrown on sugarcane bagasse pith.
Sixteen co-cultures composed of four bacteria and four fungi grown on sugarcane bagasse pith were tested for phenanthrene degradation in soil. The four bacteria were identified as Pseudomonas aeruginose, Ralstonia pickettii, Pseudomonas sp. and Pseudomonas cepacea. The four fungi were identified as: Penicillium sp., Trichoderma viride, Alternaria tenuis and Aspergillus terrus that were previously isolated from different hydrocarbon-contaminated soils. Fungi had a statistically significant positive (0.0001<p) effect on phenanthrene removal, that ranged from 35% to 50% and bacteria removed the compound by an order of 20%. Co-cultures B. cepacea-Penicillium sp., R. pickettii-Penicillium sp., and P. aeruginose-Penicillium sp. exhibited synergism for phenanthrene removal, reaching 72.84+/-3.85%, 73.61+/-6.38% and 69.47+/-4.91%; in 18 days, respectively.
Chávez-Gómez
B
B
Depto. Biotecnología y Bioingeniería, CINVESTAV-IPN, Av Instituto Politécnico Nacional 2508, AP 14-740, Col, CP 07360, Zacatenco, Mexico D F.
Quintero
R
R
Esparza-García
F
F
Mesta-Howard
A M
AM
Zavala Díaz de la Serna
F J
FJ
Hernández-Rodríguez
C H
CH
Gillén
T
T
Poggi-Varaldo
H M
HM
Barrera-Cortés
J
J
Rodríguez-Vázquez
R
R
D001419:Bacteria; D001673:Biodegradation, Environmental; D005658:Fungi; D010616:Phenanthrenes / Q000302:isolation & purification / Q000378:metabolism*; D012037:Refuse Disposal; D031786:Saccharum; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000302:isolation & purification / Q000378:metabolism*
D010616:Phenanthrenes; D012989:Soil Pollutants; C031181:phenanthrene