Expression of hemagglutinin protein of rinderpest virus in transgenic tobacco and immunogenicity of plant-derived protein in a mouse model.
The use of transgenic plants as a production system for recombinant subunit vaccines has been considered safe and economical compared to cell culture methods. We have exploited this approach to produce rinderpest virus hemagglutinin (H) protein in transgenic tobacco as a model plant for testing the immunogenicity of plant-derived hemagglutinin protein. The transgenic nature of the plants was confirmed by molecular analysis such as gene specific PCR and Southern hybridization using full-length H gene as a probe. The Mendelian pattern of inheritance of the transgene has been demonstrated in T(1) generation. The transgenic plants express the H protein of molecular weight 72 kDa. The plant derived H protein is antigenically authentic as revealed by reactivity with H-specific antibodies as well as convalescent sera. The induction of immune response was tested in mice after intraperitoneal immunization with plant-derived H. High titers of antibodies were induced which were H-specific and they neutralized the infectivity of rinderpest virus.
Khandelwal
Abha
A
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560 012, India.
Sita G
Lakshmi
L
Shaila
M S
MS
D000818:Animals; D005260:Female; D005822:Genetic Vectors; D006389:Hemagglutinins, Viral / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D030821:Plants, Genetically Modified; D011994:Recombinant Proteins / Q000276:immunology; D012302:Rinderpest virus; D014026:Nicotiana / Q000235:genetics*; D014170:Transformation, Genetic
D006389:Hemagglutinins, Viral; D011994:Recombinant Proteins