title: Genetic screen for small body size mutants in C. elegans reveals many TGFbeta pathway components.
abstract: In the nematode Caenorhabditis elegans, a TGFbeta-related signaling pathway regulates body size and male tail morphogenesis. We sought to identify genes encoding components or modifiers of this pathway in a large-scale genetic screen. Remarkably, this screen was able to identify essentially all core components of the TGFbeta signaling pathway. Among 34 Small mutants, many mutations disrupt genes encoding recognizable components of the TGFbeta pathway: DBL-1 ligand, DAF-4 type II receptor, SMA-6 type I receptor, and SMA-2, SMA-3, and SMA-4 Smads. Moreover, we find that at least 11 additional complementation groups can mutate to the Small phenotype. Four of these 11 genes, sma-9, sma-14, sma-16, and sma-20 affect male tail morphogenesis as well as body size. Two genes, sma-11 and sma-20, also influence regulation of the developmentally arrested dauer larval stage, suggesting a role in a second characterized TGFbeta pathway in C. elegans. Other genes may represent tissue-specific factors or parallel pathways for body size control. Because of the conservation of TGFbeta signaling pathways, homologs of these genes may be involved in tissue specificity and/or crosstalk of TGFbeta pathways in other animals.
authors.affiliation: Waksman Institute, Department of Molecular Biology and Biochemistry, Rutgers University, 190 Frelinghuysen Road, Piscataway, NJ 08854-8020, USA.
authors.forename: Cathy
authors.initials: C
authors.lastname: Savage-Dunn
authors.forename: Lisa L
authors.initials: LL
authors.lastname: Maduzia
authors.forename: Cole M
authors.initials: CM
authors.lastname: Zimmerman
authors.forename: Andrew F
authors.initials: AF
authors.lastname: Roberts
authors.forename: Stephen
authors.initials: S
authors.lastname: Cohen
authors.forename: Rafal
authors.initials: R
authors.lastname: Tokarz
authors.forename: Richard W
authors.initials: RW
authors.lastname: Padgett
mesh_terms: D000818:Animals; D001824:Body Constitution / Q000235:genetics*; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D008297:Male; D013623:Tail / Q000002:abnormalities; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism*
chemical_list: D016212:Transforming Growth Factor beta