title: A small yeast RNA inhibits HCV IRES mediated translation and inhibits replication of poliovirus in vivo.
abstract: OBJECTIVE
  To investigate the anti-virus infection activity of internal ribosome entry site (IRES) specific inhibitor RNA (IRNA).
  
  
  METHODS
  IRNA eukaryotic vector pcRz-IRNA or mIRNA eukaryotic vector pcRz-mIRNA was transfected into human hepatocarcinoma cells (HHCC), then selected with neomycin G418 for 4 to 8 weeks, and then infected with polio virus vaccines line. The cytopethogenesis effect was investigated and the cell extract was collected. At last the polio virus titer of different cells was determined by plaque assay.
  
  
  RESULTS
  Constructive expression of IRNA was not detrimental to cell growth. HCV IRES-mediated cap-independent translation was markedly inhibited in cells constructively expressing IRNA compared to control hepatoma cells. However, cap-dependent translation was not significantly affected in these cell line. Additionally, HHCC cells constitutively expressing IRNA became refractory to infection of polio virus.
  
  
  CONCLUSIONS
  IRES specific IRNA can inhibit HCV IRES mediated translation and poliovirus replication.
authors.affiliation: The center of diagnosis and treatment for infectious diseases of Tangdu Hospital of Military Medical University of PLA, Xi'an. 710038, Shaanxi Province, China.
authors.forename: Xue-Song
authors.initials: XS
authors.lastname: Liang
authors.forename: Jian-Qi
authors.initials: JQ
authors.lastname: Lian
authors.forename: Yong-Xing
authors.initials: YX
authors.lastname: Zhou
authors.forename: Qing-He
authors.initials: QH
authors.lastname: Nie
authors.forename: Chun-Qiu
authors.initials: CQ
authors.lastname: Hao
mesh_terms: D020121:5' Untranslated Regions; D003588:Cytopathogenic Effect, Viral / Q000235:genetics; D015870:Gene Expression; D005814:Genes, Viral; D016174:Hepacivirus / Q000235:genetics*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics; D017955:Poliovirus / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology*; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics; D012331:RNA, Fungal / Q000235:genetics*; D034741:RNA, Small Interfering; D012367:RNA, Viral / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014162:Transfection; D014407:Tumor Cells, Cultured; D014779:Virus Replication / Q000235:genetics
chemical_list: D020121:5' Untranslated Regions; D035683:MicroRNAs; D012331:RNA, Fungal; D034741:RNA, Small Interfering; D012367:RNA, Viral; D012313:RNA