NF-kappaB activation in peripheral blood mononuclear cells in neonatal asphyxia.
Neonatal asphyxia results in hypoxic-ischaemic encephalopathy. Previous studies have demonstrated that brain hypoxia and ischaemia lead to the production of proinflammatory cytokines, including tumour necrosis factor-alpha (TNF-alpha), interleukin-1 (IL-1) and IL-6. Transcription factor NF-kappaB is essential for the expression of these cytokines. We examined whether or not NF-kappaB is activated in peripheral mononuclear cells (PBMC) in neonatal asphyxia by flow cytometry. In addition, we examined the relationship between NF-kappaB activation in PBMC and the neurological prognosis. Flow cytometry analysis demonstrated that the level of NF-kappaB activation in CD14+ monocytes/macrophages of the patients with asphyxia who had neurological sequelae was significantly higher than in the controls, and in the patients with asphyxia who survived (31.7 +/- 7.2%versus 2.5 +/- 0.9%, P = 0.008, and versus 1.6 +/- 1.4%, P = 0.014, respectively). Our findings suggest that NF-kappaB activation in peripheral blood CD14+ monocytes/macrophages in neonatal asphyxia is important for predicting the subsequent neurological sequelae.
Hasegawa
K
K
Department of Paediatrics, Yamaguchi University School of Medicine, Ube, Yamaguchi, Japan.
Ichiyama
T
T
Isumi
H
H
Nakata
M
M
Sase
M
M
Furukawa
S
S
D001238:Asphyxia Neonatorum / Q000276:immunology*; D015415:Biomarkers / Q000032:analysis; D016022:Case-Control Studies; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007231:Infant, Newborn; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism*; D018950:Lipopolysaccharide Receptors / Q000276:immunology; D008297:Male; D016328:NF-kappa B / Q000378:metabolism*; D011379:Prognosis
D015415:Biomarkers; D018950:Lipopolysaccharide Receptors; D016328:NF-kappa B