Isolation of a new hemimethylated DNA binding protein which regulates dnaA gene expression.
In this report, we show that yccV, a gene of unknown function, encodes a protein having an affinity for a hemimethylated oriC DNA and that the protein negatively controls dnaA gene expression in vivo.
d'Alençon
Emmanuelle
E
Institut J. Monod, Université Paris VI-VII, 75251 Paris Cedex 05, France.
Taghbalout
Aziz
A
Bristow
Claire
C
Kern
Renée
R
Aflalo
Revital
R
Kohiyama
Masamichi
M
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D019175:DNA Methylation; D004261:DNA Replication; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D015536:Down-Regulation; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D008969:Molecular Sequence Data
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; C066312:DnaA protein, Bacteria