Approaches to proteomic analysis of human tumors.
The completion of the Human Genome Project and the successful use of high-throughput array formats for mRNA measurements has spurred a revival of interest in the field of proteomics. Clinical cancer specimens will be a critical component of proteomic studies of cancer, both for discovering new insights into TSG function, and for validation of discoveries that are made in the laboratory. Global protein analysis methods hold great potential for facilitating the discovery of novel tumor suppressor genes, as well as providing a better understanding of the biochemical role of these proteins.
Ahram
Mamoun
M
Molecular Biosciences, Pacific Northwest National Laboratory, Battelle, Richland, WA, USA.
Emmert-Buck
Michael R
MR
D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D006801:Humans; D009369:Neoplasms / Q000378:metabolism*; D040081:Protein Array Analysis; D020543:Proteome / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D020543:Proteome