title: A new in vitro tissue factor inhibitory triterpene from the fruits of Chaenomeles sinensis.
abstract: Tissue factor (TF, tissue thromboplastin) accelerates the blood clotting, activating both the intrinsic and the extrinsic pathways to serve as a cofactor. In order to isolate TF inhibitors from the fruits of Chaenomeles sinensis, an activity-guided purification was carried out to yield four triterpenoid compounds. One compound was new and its structure was elucidated as 28-O-beta- D-glucopyranosyl-2alpha,3beta-dihydroxyolean-12-ene-24,28-dioic acid (2), named chaenomeloside A by means of spectral analysis and chemical conversion. Other compounds were trachelosperoside A-1 (1), oleanolic acid (3) and ursolic acid (4). Compound 2 and its aglycone 2a, named chaenomelogenin A inhibited by 50 % the TF activity at concentrations of 0.036 and 0.028 mM/unit of TF, respectively. Compound 1 isolated for the first time from this plant as well as 3 and 4 were inactive.
authors.affiliation: Natural Products Research Institute, Seoul National University, Seoul, Korea.
authors.forename: Ming Hong
authors.initials: MH
authors.lastname: Lee
authors.forename: Yong Nam
authors.initials: YN
authors.lastname: Han
mesh_terms: D000818:Animals; D005638:Fruit; D020128:Inhibitory Concentration 50; D008168:Lung / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D027824:Rosaceae; D013925:Thromboplastin / Q000037:antagonists & inhibitors / Q000187:drug effects*; D014315:Triterpenes / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D010936:Plant Extracts; D014315:Triterpenes; D013925:Thromboplastin