Mutagenic and genotoxic evaluation of bisphenol F diglycidyl ether (BFDGE) in prokaryotic and eukaryotic systems.
The epoxy resin bisphenol F diglycidyl ether (BFDGE), was examined for its mutagenicity in prokaryotic assays (Salmonella typhimurium His(-) and Escherichia coli Trp(-) tests) and its genotoxicity in eukaryotic systems (sister chromatid exchange (SCE) and micronucleus tests in human lymphocytes), in the presence or absence of an exogenous metabolizing system (S9 from rat liver). In the prokaryotic tests, the concentrations of BFDGE ranged between 100 and 5000 micro g per plate, and in the eukaryotic assays from 12.5 to 62.5 micro g/ml. The compound is able to induce mutagenic effects in bacterial strains TA100, TA1535, WP2uvrA and IC3327, as revealed by the increase observed in the number of induced revertants. With respect to the genotoxicity assays, BFDGE induces an increase in the frequency of sister chromatid exchanges and micronuclei in human peripheral blood lymphocytes.
Sueiro
Rosa Ana
RA
Laboratorio de Microbioloxía, Instituto de Investigación e Análises Alimentarias, Universidade de Santiago de Compostela, Campus Sur s/n, 15782, Santiago de Compostela, Spain. mprosaan@usc.es
Suárez
Susanna
S
Araujo
Manuel
M
Garrido
Manuel Joaquín
MJ
D001559:Benzhydryl Compounds; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004852:Epoxy Compounds / Q000633:toxicity*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D005057:Eukaryotic Cells / Q000187:drug effects*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000502:physiology; D015162:Micronucleus Tests; D009152:Mutagenicity Tests / Q000379:methods; D011387:Prokaryotic Cells / Q000187:drug effects*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D012854:Sister Chromatid Exchange / Q000187:drug effects
D001559:Benzhydryl Compounds; D004852:Epoxy Compounds; C423722:bisphenol F diglycidyl ether