title: Implant surface roughness affects osteoblast gene expression.
abstract: The transcription factor Cbfa1 regulates osteoblast differentiation and expression of genes necessary for the development of a mineralized phenotype. The purpose of this study was to determine if Cbfa1 and BSPII gene expression are influenced by implant surface microtopography. Osteoblasts were cultured on 600-grit (grooved) or sandblasted (roughened) cpTi implant discs. Mineralization was evaluated by Alizarin-Red-S staining. Real Time PCR was used for quantitative analysis of Cbfa1 and BSPII gene expression. Enhanced mineralization was seen in osteoblasts grown on roughened implant surfaces relative to tissue culture plastic. Real Time PCR showed significant (P < 0.05) increases in Cbfa1 gene expression in cells grown on roughened, as compared with grooved, implant surfaces. BSPII gene expression was also increased on rough surfaces in the UMR cells, but was reduced in the rat calvarial osteoblast cultures. These results suggest that osteoblast gene expression and mineralization are affected by roughened implant surface microtopographies during osseointegration of dental implants.
authors.affiliation: N402, Dows Institute for Dental Research and the Department of Prosthodontics, University of Iowa College of Dentistry, Iowa City 52242, USA. galen-schneider@uiowa.edu
authors.forename: G B
authors.initials: GB
authors.lastname: Schneider
authors.forename: H
authors.initials: H
authors.lastname: Perinpanayagam
authors.forename: M
authors.initials: M
authors.lastname: Clegg
authors.forename: R
authors.initials: R
authors.lastname: Zaharias
authors.forename: D
authors.initials: D
authors.lastname: Seabold
authors.forename: J
authors.initials: J
authors.lastname: Keller
authors.forename: C
authors.initials: C
authors.lastname: Stanford
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002113:Calcification, Physiologic; D002454:Cell Differentiation; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit; D015921:Dental Implants; D015870:Gene Expression; D058574:Integrin-Binding Sialoprotein; D008855:Microscopy, Electron, Scanning; D009363:Neoplasm Proteins; D016348:Osseointegration / Q000502:physiology*; D010006:Osteoblasts / Q000378:metabolism*; D051381:Rats; D012795:Sialoglycoproteins / Q000096:biosynthesis*; D018709:Statistics, Nonparametric; D013499:Surface Properties; D014025:Titanium; D014157:Transcription Factors / Q000096:biosynthesis*
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; D015921:Dental Implants; C546873:Ibsp protein, rat; D058574:Integrin-Binding Sialoprotein; D009363:Neoplasm Proteins; D012795:Sialoglycoproteins; D014157:Transcription Factors; D014025:Titanium