title: Quality of packed red blood cells and platelet concentrates collected by multicomponent collection using the MCS plus device.
abstract: The demand for blood components is constantly increasing, while the exclusion criteria for donors are strengthened in order to reach maximal safety for donors and patients. To counterbalance reduced availability of volunteers, multicomponent collections (MCC) is an attractive approach to produce more than one component during a single apheresis procedure from one donor, such as packed red blood cells (PRBCs) and platelet concentrates (PCs). Further, the exposures of patients to a limited number of donors reduces the possibility of alloimmunization and transfusion-related diseases. We measured the quality of PRBCs and PCs obtained by MCC, using the MCS+ device with the LDPRBC program, Revision B, and compared them with the quality of manually collected PRBCs and PCs collected with the Revision C2 of the MCS+. We found higher pH levels and lower hemolysis assessed by means of fHb and K+ in the supernatant of PRBCs over the whole storage period of 42 days in MCC-derived PRBCs. The functional metabolism assessed by intracellular ATP was higher in PRBCs collected by MCC than in manually collected units. Furthermore, PCs obtained during MCC showed an increase in p-selectin expression on day 5 of storage compared to PCs collected with the Revision C2 of the MCS+. The p-selectin expression on MCC platelets was within the range of p-selectin expression found in PCs obtained by other apheresis devices. These results indicate less storage lesion in MCC-derived PRBCs compared to manually collected units and no compromise in the quality of MCC PCs obtained in the same apheresis procedure.
authors.affiliation: Department of Blood Group Serology and Transfusion Medicine, University of Vienna, Vienna, Austria. gerda.leitner@univie.ac.at
authors.forename: G C
authors.initials: GC
authors.lastname: Leitner
authors.forename: P
authors.initials: P
authors.lastname: Jilma-Stohlawetz
authors.forename: G
authors.initials: G
authors.lastname: Stiegler
authors.forename: G
authors.initials: G
authors.lastname: Weigel
authors.forename: M
authors.initials: M
authors.lastname: Horvath
authors.forename: A
authors.initials: A
authors.lastname: Tanzmann
authors.forename: P
authors.initials: P
authors.lastname: Höcker
authors.forename: M B
authors.initials: MB
authors.lastname: Fischer
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis; D000328:Adult; D001792:Blood Platelets; D001793:Blood Preservation; D016238:Cytapheresis / Q000295:instrumentation* / Q000379:methods* / Q000592:standards; D017707:Erythrocyte Transfusion / Q000295:instrumentation / Q000379:methods / Q000592:standards; D004912:Erythrocytes; D005319:Fetal Hemoglobin / Q000032:analysis; D006461:Hemolysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D010983:Plateletpheresis / Q000295:instrumentation / Q000379:methods / Q000592:standards; D011188:Potassium / Q000032:analysis; D011786:Quality Control; D013997:Time Factors
chemical_list: D000255:Adenosine Triphosphate; D005319:Fetal Hemoglobin; D011188:Potassium