Analysis of a shared pancreatic cancer antigen recognized by an HLA-A*2601-restricted cytotoxic T-lymphocyte clone.
BACKGROUND
We have generated HLA-A*2601-restricted CD8+ CTL clones against an autologous pancreatic cancer cell line.


OBJECTIVE
To characterize the antigen expressed on the cancer cells.


METHODS
We assessed cytotoxic activities and cytokine production of these CTL clones reacting against cancer cell lines that stably or transiently expressed the HLA-A*2601 gene.


RESULTS
These CTL clones recognized 4 of 10 allogeneic pancreatic cancer cell lines and a gallbladder cancer cell line in the context of HLA-A*2601. However, the CTL clones did not recognize three hepatocellular carcinoma cell lines, two esophageal squamous cell carcinoma cell lines, or a lung adenocarcinoma cell line.


CONCLUSIONS
Thus, the CTL clones may recognize a shared, but not ubiquitously expressed, tumor antigen on pancreatic and gallbladder cancer cells.
Kashiwagi
Kiyoteru
K
Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, Japan.
Ikeda
Hideyuki
H
Hirohashi
Yoshihiko
Y
Yamamoto
Masayuki
M
Idenoue
Satomi
S
Hirai
Itaru
I
Kamiguchi
Kenjiro
K
Tamura
Yasuaki
Y
Torigoe
Toshiko
T
Wada
Yshimasa
Y
Hirata
Koichi
K
Sato
Noriyuki
N
D000951:Antigens, Neoplasm / Q000032:analysis / Q000276:immunology*; D045744:Cell Line, Tumor; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D005838:Genotype; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D015234:HLA-A Antigens / Q000235:genetics / Q000276:immunology*; D006801:Humans; D020014:K562 Cells; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology*
D000951:Antigens, Neoplasm; D015234:HLA-A Antigens; C039683:HLA-A*26 antigen; D016178:Granulocyte-Macrophage Colony-Stimulating Factor