Involvement of normal physiological mechanisms in mediation of satiety by polyherbal antiobesity preparation, OB-200G, in female mice.
The first study investigated the effect of different doses (0.25, 0.5, 1 g/kg p.o.) of a polyherbal, antiobesity preparation, OB-200G, on the duration of a specific sequence of behaviors (activity, feeding, grooming and resting) associated with the onset of satiety. The recordings were taken at 0, 30 and 60 min, for 10-min periods, immediately after the presentation of sweetened chow to female mice that had been fasted for 16 h. At the end of a 70-min test session, the amount of sweetened chow consumed by each mouse was also recorded. The second study investigated the antagonism of hyperphagia mediated by 2-deoxy-D-glucose (2-DODG), by both OB-200G (0.5 g/kg) and fluoxetine (10 mg/kg). Ingestion of sweetened chow by control mice produced the typical sequence of behaviors associated with the onset of satiety, characterized by initial increase in duration of feeding and active behaviors. With time, these behaviors declined and there was an increase in grooming and resting behaviors. Prefeeding significantly (p < 0.01) decreased the percentage of time spent feeding but also significantly (p < 0.01) increased the duration of active behaviors at 0-10 and 30-40 min. Prefeeding for 20 min advanced the onset and increased the duration of grooming behavior. Treatment with fluoxetine (10 mg/kg) significantly decreased the duration of feeding behavior and increased the active behavior duration at 0-10 and 30-40 min as compared with the control group. There was also a significant (p < 0.01) increase in resting behavior duration with fluoxetine at 60-70 min. OB-200G (0.25-1 g/kg) administration significantly decreased the feeding behavior duration and increased the active-behavior duration at 30-40 min as compared with the control group during that period. However, OB-200G (0.25-I g/kg) did not elicit any significant change in grooming (except decrease at 60-70 min with a 0.5 g/kg dose) and resting behavior duration compared with the control or prefed groups. The amount of sweetened chow consumed significantly decreased (p < 0.01) in 10 and 20 min prefed, fluoxetine-treated and OB-200G-treated (0.5 and 1 g/kg) mice as compared with the control group. Furthermore, the hyperphagia mediated by 2-DODG was significantly antagonized by both OB-200G and fluoxetine. In conclusion, like fluoxetine, OB-200G mediates satiety through operation of normal physiological mechanisms. Antagonism of 2-DODG-hyperphagia by OB-200G and fluoxetine indicates the involvement of a common central pathway (or pathways) in their action.
Kaur
G
G
Pharmacology Division, University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh, India.
Kulkarni
S K
SK
D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology*; D004435:Eating / Q000187:drug effects* / Q000502:physiology; D005260:Female; D005473:Fluoxetine / Q000494:pharmacology*; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D011930:Reaction Time / Q000187:drug effects / Q000502:physiology; D012528:Satiety Response / Q000187:drug effects* / Q000502:physiology
D019440:Anti-Obesity Agents; C471887:OB200G polyherbal preparation; D010936:Plant Extracts; D005473:Fluoxetine