Enhanced blood compatibility of polymers grafted by sulfonated PEO via a negative cilia concept.
In our laboratory sulfonated PEO (PEO-SO(3)) was designed as a "negative cilia model" to investigate a synergistic effect of PEO and negatively charged SO(3) groups. PEO-SO(3) itself exhibited a heparin-like anticoagulant activity of 14% of free heparin. Polyurethane grafted with PEO-SO(3) (PU-PEO-SO(3)) increased the albumin adsorption to a great extent but suppressed other proteins, while PU-PEO decreased the adsorption of all the proteins. The platelet adhesion was decreased on PU-PEO but least on PU-PEO-SO(3) to demonstrate an additional effect of SO(3) groups. The enhanced blood compatibility of PU-PEO-SO(3) in the ex vivo rabbit and in vivo canine implanting tests was confirmed. Furthermore, PU-PEO-SO(3) exhibited an improved biostability and suppressed calcification in addition to the enhanced antithrombogenicity. The in vivo antithrombogenicity and biostability were improved in the order of PU<PU-PEO<PU-PEO-SO(3). The calcium amounts deposited was decreased in the order of PU>PU-PEO>PU-PEO-SO(3) in spite of the possible attraction between negative SO(3) groups and positive calcium ions. The bioprosthetic tissue (BT) was grafted with H(2)N-PEO-SO(3) via glutaraldehyde (GA) residues after conventional GA fixation. BT-PEO-SO(3) also displayed the decreased calcification by in vivo animal models. The application of PEO-SO(3) was extended by designing amphiphilic copolymers containing PEO-SO(3) moiety and hydrophobic long alkyl groups as anchors. The superior effect of PEO-SO(3) groups on thromboresistance compared to PEO was confirmed also in the case of copolymers coated or blended with other polymers and the systems coupled by UV irradiation, photoreaction or gold/sulfur or silane coupling technology, and therefore it might be very useful for the medical devices.
Kim
Young Ha
YH
Biomaterials Research Center, Korea Institute of Science and Technology, Seoul 130-650, Republic of Korea. yhakim@kist.re.kr
Han
Dong Keun
DK
Park
Ki Dong
KD
Kim
Soo Hyun
SH
D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry*; D001777:Blood Coagulation / Q000502:physiology*; D001790:Blood Physiological Phenomena; D001798:Blood Proteins / Q000737:chemistry*; D006648:Histocompatibility; D006801:Humans; D008422:Materials Testing; D015539:Platelet Activation / Q000502:physiology; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D013451:Sulfonic Acids / Q000737:chemistry*; D013499:Surface Properties; D013927:Thrombosis / Q000517:prevention & control*; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*; D019737:Transplants
D001672:Biocompatible Materials; D001798:Blood Proteins; D011108:Polymers; D013451:Sulfonic Acids; C426021:amino-polyethyleneoxide-sulfonate; D011092:Polyethylene Glycols