title: Extracellular vestibule determinants of Ca2+ influx in Ca2+-permeable AMPA receptor channels.
abstract: At certain synapses in the brain, Ca2+-permeable AMPA receptor (AMPAR) channels represent an important pathway for synaptically controlled Ca2+ entry. However, the molecular determinants of this Ca2+ influx are poorly defined. In NMDA receptor (NMDAR) channels, where the influx is much greater, the extracellular vestibule, specifically the M3 segment and regions C-terminal to it in the NR1 subunit, contains elements critical to their high Ca2+ influx under physiological conditions. We therefore investigated the contribution of homologous positions in AMPAR as well as kainate receptor (KAR) subunits to the process of Ca2+ influx. Substitutions of a conserved asparagine (N) in M3 of AMPAR GluR-B(Q) channels strongly attenuated Ca2+ permeability measured using reversal potentials under biionic conditions and fractional Ca2+ currents recorded under physiological conditions. Hence, as in NMDAR channels, the conserved N makes a significant contribution to Ca2+ influx in AMPAR channels. In addition, C-terminal to M3, substitutions of negatively (glutamate, E) or positively (arginine, R) charged residues also altered Ca2+ influx. However, in contrast to charged residues occupying homologous positions in NMDAR channels, these effects were about equal and opposite suggesting that this ER in AMPARs does not contribute significantly to the mechanism of Ca2+ influx. Opposite charge substitutions of two negative residues C-terminal to M3 in KAR GluR-6(Q) subunits had no effect on Ca2+ permeability. We conclude that the different contribution of residues C-terminal to M3 to Ca2+ permeation in NMDAR and non-NMDAR channels reflects a different positioning of these residues relative to the tip of the M2 loop.
authors.affiliation: Department of Neurobiology and Behavior, State University of New York at Stony Brook, Stony Brook, NY 11794-5230, USA.
authors.forename: Claudia
authors.initials: C
authors.lastname: Jatzke
authors.forename: Matthew
authors.initials: M
authors.lastname: Hernandez
authors.forename: Lonnie P
authors.initials: LP
authors.lastname: Wollmuth
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D019943:Amino Acid Substitution; D001120:Arginine; D001216:Asparagine; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D017124:Conserved Sequence / Q000235:genetics; D004553:Electric Conductivity; D005110:Extracellular Space / Q000502:physiology*; D018698:Glutamic Acid; D006801:Humans; D007473:Ion Channels / Q000378:metabolism*; D008969:Molecular Sequence Data; D010539:Permeability; D018091:Receptors, AMPA / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D018092:Kainic Acid Receptors / Q000235:genetics / Q000502:physiology; D000097806:GluK2 Kainate Receptor
chemical_list: D001120:Arginine; D001216:Asparagine; D002118:Calcium; D018698:Glutamic Acid; D007473:Ion Channels; D018091:Receptors, AMPA; D018092:Kainic Acid Receptors; D000097806:GluK2 Kainate Receptor; C115953:glutamate receptor type B