[Migration and differentiation of human bone marrow mesenchymal stem cells in the rat brain].
Bone marrow mesenchymal stem cells (MSCs) are multipotent tissue stem cells that can be induced in vitro to differentiate into a variety of cells such as osteoblasts, chondrocytes and adipocytes. MSCs are useful vehicles for both cell and gene therapy for a variety of diseases. Here, we injected human MSCs with enhanced green fluorescent protein (EGFP) into the striatum of Parkinson disease (PD) rat and examined their survival, migration, differentiation, and the behavior changes in PD rats, which will provide a theoretical foundation and technical method for clinic PD therapy by stem cells. The results showed that human bone marrow MSCs can survive in rat brain for a long time (exceeding 70 d). MSCs were found in multiple areas of the rat brain including the striatum, the corpus callosum, contralateral cortex and even the brain vascular wall. Immunocytochemical staining suggested that implanted cells expressed human neurofilament (NF), neuron-specific enolase (NSE) and glial fibrillary acid protein (GFAP). At the same time, remission in abnormal behavior of the PD rats appeared. Rotation scores decreased gradually from 8.86+/-2.09 r/min pre-transplantation to 4.87+/-2.06 r/min 90 d post-transplantation (statistic result showed P<0.05).
Hou
Ling-Ling
LL
Beijing Institute of Transfusion Medicine, Beijing, PR China.
Zheng
Min
M
Wang
Dong-Mei
DM
Yuan
Hong-Feng
HF
Li
Hai-Min
HM
Chen
Lin
L
Bai
Ci-Xian
CX
Zhang
Yong
Y
Pei
Xue-Tao
XT
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation; D002465:Cell Movement; D003342:Corpus Striatum; D049452:Green Fluorescent Proteins / Q000008:administration & dosage; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010300:Parkinson Disease / Q000628:therapy*; D051381:Rats; D017208:Rats, Wistar; D033581:Stem Cell Transplantation / Q000379:methods*; D014183:Transplantation, Heterologous
C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins