title: Used leucodepletion filters as a source of large quantities of DNA suitable for the study of genetic variations in human populations.
abstract: A simple technique for developing large control panels with large quantities of DNA suitable for studies in population genetics was established.
  
  
  OBJECTIVE
  Both a lack of suitable controls and insufficient quantities of DNA for repeated analysis of the same control group often hamper the investigation of genetic markers for disease.
  
  
  METHODS
  Using a waste product from routine blood donation, we describe a simple method that allows the investigator to extract large amounts of DNA.
  
  
  RESULTS
  A mean of 1520 microg of DNA per sample was obtained. The DNA obtained remains suitable for polymerase chain reaction and sequencing techniques after 2 years of storage at both 4 degrees C and -40 degrees C.
  
  
  CONCLUSIONS
  This technique allows the development of a large panel of controls with sufficient quantities of genomic DNA for thousands of tests.
authors.affiliation: H&I, National Blood Service and Cancer Research UK Institute for Cancer Studies, University of Birmingham, Edgbaston, Birmingham, UK. mark.cook@nbs.nhs.uk
authors.forename: M A
authors.initials: MA
authors.lastname: Cook
authors.forename: S E
authors.initials: SE
authors.lastname: Jobson
authors.forename: D C
authors.initials: DC
authors.lastname: Atkinson
authors.forename: D P
authors.initials: DP
authors.lastname: Lowe
authors.forename: S L
authors.initials: SL
authors.lastname: Farmer
authors.forename: W J
authors.initials: WJ
authors.lastname: Alvi-Ali
authors.forename: N A
authors.initials: NA
authors.lastname: Smith
authors.forename: D C
authors.initials: DC
authors.lastname: Briggs
mesh_terms: D001782:Blood Donors; D002469:Cell Separation / Q000379:methods; D004247:DNA / Q000302:isolation & purification*; D005374:Filtration; D005819:Genetic Markers; D036281:Genetic Research; D014644:Genetic Variation; D006801:Humans; D007962:Leukocytes; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D005819:Genetic Markers; D004247:DNA