Isoprenoid biosynthesis via the methylerythritol phosphate pathway: the (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase (LytB/IspH) from Escherichia coli is a [4Fe-4S] protein.
The last enzyme (LytB) of the methylerythritol phosphate pathway for isoprenoid biosynthesis catalyzes the reduction of (E)-4-hydroxy-3-methylbut-2-enyl diphosphate into isopentenyl diphosphate and dimethylallyl diphosphate. This enzyme possesses a dioxygen-sensitive [4Fe-4S] cluster. This prosthetic group was characterized in the Escherichia coli enzyme by UV/visible and electron paramagnetic resonance spectroscopy after reconstitution of the purified protein. Enzymatic activity required the presence of a reducing system such as flavodoxin/flavodoxin reductase/reduced nicotinamide adenine dinucleotide phosphate or the photoreduced deazaflavin radical.
Wolff
Murielle
M
Université Louis Pasteur, UMR CNRS 7123, Institut Le Bel, Strasbourg, France.
Seemann
Myriam
M
Tse Sum Bui
Bernadette
B
Frapart
Yves
Y
Tritsch
Denis
D
Garcia Estrabot
Ana
A
Rodríguez-Concepción
Manuel
M
Boronat
Albert
A
Marquet
Andrée
A
Rohmer
Michel
M
D011756:Diphosphates / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D004896:Erythritol / Q000031:analogs & derivatives* / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000378:metabolism*; D045782:Hemiterpenes; D007506:Iron-Sulfur Proteins / Q000737:chemistry / Q000378:metabolism*; D008956:Models, Chemical; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism*; D010088:Oxidoreductases / Q000737:chemistry / Q000378:metabolism*; D011105:Polyisoprenyl Phosphate Sugars / Q000096:biosynthesis*; D013403:Sugar Phosphates / Q000378:metabolism*
C114232:2-C-methylerythritol 4-phosphate; C443995:4-hydroxy-3-methylbut-2-enyl pyrophosphate; D011756:Diphosphates; D029968:Escherichia coli Proteins; D045782:Hemiterpenes; D007506:Iron-Sulfur Proteins; D009943:Organophosphorus Compounds; D011105:Polyisoprenyl Phosphate Sugars; D013403:Sugar Phosphates; C004809:isopentenyl pyrophosphate; D010088:Oxidoreductases; C109866:ispH protein, E coli; D009247:NADH, NADPH Oxidoreductases; C116041:flavodoxin NADPH oxidoreductase; D004896:Erythritol