Construction and characterization of pentacistronic retrovirus vectors.
The picornavirus foot-and-mouth disease virus 2A sequence was combined with three different internal ribosome entry segments to construct and characterize three independent pentacistronic retroviruses of different sizes. Efficient co-expression of the five proteins was successful and titres obtained for these pentacistronic virus vectors (final genome size approximately 7.9 kb) were comparable to those of vector systems with shorter genomes. Other vectors constructed that exceeded the genome length of the wild-type virus suffered frequent deletions.
Felipe
Pablo de
P
Departamento de Bioquímica y Biología Molecular-Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid, Facultad de Ciencias, Cantoblanco, 28049 Madrid, Spain.
Izquierdo
Marta
M
Departamento de Bioquímica y Biología Molecular-Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid, Facultad de Ciencias, Cantoblanco, 28049 Madrid, Spain.
D016475:3T3 Cells; D000818:Animals; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics* / Q000378:metabolism; D017930:Genes, Reporter / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D012190:Retroviridae / Q000235:genetics*; D012270:Ribosomes / Q000235:genetics* / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
D008164:Luminescent Proteins; D014764:Viral Proteins; D049452:Green Fluorescent Proteins