Analysis of the Plasmodium vivax apical membrane antigen-1 gene from re-emerging Korean isolates.
Apical membrane antigen-1 is a candidate vaccine component for malaria. In the present study, we investigated the polymorphism of the Plasmodium vivax apical membrane antigen-1 gene ( PvAMA-1) in 30 Korean isolates. The polymorphic region of the PvAMA-1 gene, corresponding to nucleotides 324-735 (aa 108-245), was amplified using polymerase chain reaction followed by cloning and sequencing. Two genotypes ( SKA and SKG) were identified on the basis of amino acid substitution. These were identical to those of the Chinese isolates. The Korean isolates showed sequence variation at six positions on the basis of the sequence of the Sal1 strain. Of these, variation at position 189 (Glu/Lys) was found only in SKA. These two genotypes were related to the genotype of the circumsporozoite and Duffy binding protein of the Korean isolate. These findings suggest that two genotypes of P. vivax coexist in the endemic area and that the re-emerging parasite in Korea may be related to or have originated in East Asia.
Chung
Joon-Yong
JY
Department of Parasitology and Institute of Malariology, Inje University College of Medicine, Pusan 614-735, Republic of Korea.
Chun
Eui-Hyun
EH
Chun
Jin-Ho
JH
Kho
Weon-Gyu
WG
D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D021821:Communicable Diseases, Emerging / Q000453:epidemiology / Q000469:parasitology*; D006801:Humans; D007723:Korea / Q000453:epidemiology; D016780:Malaria, Vivax / Q000453:epidemiology / Q000469:parasitology*; D008297:Male; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010966:Plasmodium vivax / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics*; D017422:Sequence Analysis, DNA
D000953:Antigens, Protozoan; D008565:Membrane Proteins; D015800:Protozoan Proteins; C090077:apical membrane antigen I, Plasmodium