[Studies on the homing-related adhesion activities of UCB HSPC in short-term ex vivo expansion].
OBJECTIVE
To study the effect of ex vivo expansion on the adhesion activities of umbilical cord blood hematopoietic stem and progenitor cells (HSPC).


METHODS
Fresh UCB CD(34)(+) cells were cultured in a serum and stroma-free culture system. At day 7, day 10 and day 14, CD(34)(+) cells were re-selected from the expanded products. The expression of adhesion molecules (CAMs) such as VLA-4, VLA-5, LFA-1, ICAM-1, HCAM, L-selectin and PECAM-1, and the adhesion activity of the expanded CD(34)(+) cells were evaluated and compared with those of precultured fresh CD(34)(+) cells.


RESULTS
(1) The CD(34)(+) cells expressing homing-related CAMs were increased (from 15-fold increase for CD(34)(+) CD(54)(+) subset to 72-fold increase for CD(34)(+) CD(49e)(+) subset at day 14). (2) The expressions of CD(49d), CD(44), CD(11a) and CD(49e) on the expanded CD(34)(+) cells were increased or sustained the same levels as those on fresh UCB CD(34)(+) cells, while the expression of CD(62L), CD(54) and CD(31) on expanded CD(34)(+) cells declined with the cultivating. (3) Spontaneous adhesion and SDF-1-induced adhesion tended to be increased in the course of the first 10 day's culture.


CONCLUSIONS
The culture system used in this study could substantially support the expansion of HSPCs expressing the above CAMs, and the expanded HSPCs would sustain their intrinsic adhesion potentials.
Zhai
Qiong-li
QL
Institute of Hematology & Blood Diseases Hospital, CAMS and PUMC, Tianjin 300020, China.
Zhou
Yu
Y
Qiu
Lu-gui
LG
Li
Qian
Q
Han
Jun-ling
JL
Yu
Zhen
Z
Meng
Heng-xing
HX
Ying
Hong-guang
HG
Wang
Ya-fei
YF
Han
Zhong-chao
ZC
D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34 / Q000032:analysis; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D002455:Cell Division; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D016201:Receptors, Lymphocyte Homing / Q000096:biosynthesis*
D015703:Antigens, CD; D018952:Antigens, CD34; D015815:Cell Adhesion Molecules; D016201:Receptors, Lymphocyte Homing