DNA degradation and protein peroxidation in cells exposed to hydroxyl free radicals.
The kinetics of DNA and protein damage in two lines of cultured cells exposed to radiation-generated hydroxyl free radicals were measured. The results show that DNA damage is a relatively late event, preceded by the formation of protein hydroperoxides which may play a role in the degradation of the DNA.
Du
Juan
J
Department of Biological Sciences, Macquarie University, Sydney, New South Wales, Australia.
Gebicki
Janusz M
JM
D000818:Animals; D004247:DNA / Q000378:metabolism; D004249:DNA Damage; D053938:DNA Fragmentation; D004307:Dose-Response Relationship, Radiation; D005609:Free Radicals; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D011506:Proteins / Q000187:drug effects; D017382:Reactive Oxygen Species; D013997:Time Factors; D014407:Tumor Cells, Cultured; D020298:U937 Cells
D005609:Free Radicals; D011506:Proteins; D017382:Reactive Oxygen Species; D004247:DNA; D006861:Hydrogen Peroxide