title: [Quantitative determination of 3-nitropropionic acid by HPLC].
abstract: A reversed-phase liquid chromatographic method to determine the concentration of 3-nitropropionic acid (3-NPA) in the culture medium with Aspergillus oryzae was developed. The culture medium samples were extracted with ethyl acetate. The analytic column was Hypersil C18 and the mobile phase was CH3CN/KH2PO4(0.02 mol/L, pH 3.0) (1/3, v/v). The sample was detected at UV210 nm. The detection limit of this method was at 10 micrograms/kg level and recoveries were 90.1%-98.0.
authors.affiliation: Institute of Food Safety Control and Inspection, Ministry of Public Health China, Beijing 100021, China.
authors.forename: T
authors.initials: T
authors.lastname: Jiang
authors.forename: Q
authors.initials: Q
authors.lastname: Zhang
authors.forename: X
authors.initials: X
authors.lastname: Luo
mesh_terms: D001236:Aspergillus oryzae / Q000254:growth & development / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003470:Culture Media; D009183:Mycotoxins / Q000032:analysis*; D009574:Nitro Compounds; D011422:Propionates / Q000032:analysis*
chemical_list: D003470:Culture Media; D009183:Mycotoxins; D009574:Nitro Compounds; D011422:Propionates; C015392:3-nitropropionic acid