Selective internalization of monoclonal antibodies by B-cell chronic lymphocytic leukaemia cells.
B-cell chronic lymphocytic leukaemia (B-CLL) cannot be cured by conventional chemotherapy, therefore, toxin-linked therapeutic monoclonal antibodies (mAbs) are increasingly examined for their potential to improve clinical outcome. The current study aimed to identify mAbs that were internalized by the B-CLL cells of 14 patients, using both flow cytometry and confocal laser scanning microscopy. Anti-CD5, CD22 and CD40 mAbs were effectively taken up by B-CLL cells, whereas mAbs against CD19, CD20, CD23 and CD45 were not. This study may form a basis for further research to identify antibodies that may serve as carriers for toxins to treat B-CLL.
Sieber
Thomas
T
Biomedizinisches Forschungszentrum, mit Schwerpunkt Hepatologie und Gastroenterologie, Charité, Campus Virchow-Klinikum der Humboldt-Universität zu Berlin, Berlin, Germany.
Schoeler
Dagmar
D
Ringel
Frauke
F
Pascu
Maria
M
Schriever
Folke
F
D000911:Antibodies, Monoclonal / Q000276:immunology*; D015703:Antigens, CD / Q000276:immunology; D018941:Antigens, CD19 / Q000276:immunology; D018951:Antigens, CD20 / Q000276:immunology; D000944:Antigens, Differentiation, B-Lymphocyte / Q000276:immunology; D001402:B-Lymphocytes / Q000502:physiology*; D019013:CD40 Antigens / Q000276:immunology; D018956:CD5 Antigens / Q000276:immunology; D015815:Cell Adhesion Molecules; D004337:Drug Carriers; D004705:Endocytosis / Q000502:physiology*; D005434:Flow Cytometry; D006801:Humans; D037102:Lectins / Q000276:immunology; D015448:Leukemia, B-Cell / Q000276:immunology*; D017493:Leukocyte Common Antigens / Q000276:immunology; D018613:Microscopy, Confocal; D017455:Receptors, IgE / Q000276:immunology; D051918:Sialic Acid Binding Ig-like Lectin 2
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018941:Antigens, CD19; D018951:Antigens, CD20; D000944:Antigens, Differentiation, B-Lymphocyte; C497867:CD22 protein, human; D019013:CD40 Antigens; D018956:CD5 Antigens; D015815:Cell Adhesion Molecules; D004337:Drug Carriers; D037102:Lectins; D017455:Receptors, IgE; D051918:Sialic Acid Binding Ig-like Lectin 2; D017493:Leukocyte Common Antigens