title: Structure-expression relationship of tumor necrosis factor receptor mutants that increase expression.
abstract: The extracellular domain of the p55 TNF receptor (TNFrED) is an important therapeutic protein for targeting tumor necrosis factor-alpha (TNF-alpha). The expression level of the TNFrED is low for bioproduction, which is presumably associated with the complication of pairing 24 cysteine residues to form correct disulfide bonds. Here we report the application of the yeast display method to study expression of TNFrED, a multimeric receptor. Randomly mutated libraries of TNFrED were screened, and two mutants were identified that express several-fold higher protein levels compared with the wild type while still retaining normal binding affinity for TNF-alpha. The substituted residues responsible for the higher protein expression in both mutants were identified as proline, and both proline residues are adjacent to cysteine residues involved in disulfide bonds. Analysis of the mutant residues revealed that the improved level of expression is due to conformational restriction of the substituted residues to that of the folded state seen in the crystal structures of TNFrED thereby forcing the neighboring cysteine residues into the correct orientation for proper disulfide bond formation.
authors.affiliation: Serono Reproductive Biology Institute, Rockland, Massachusetts 02370, USA.
authors.forename: Rene L
authors.initials: RL
authors.lastname: Schweickhardt
authors.forename: Xuliang
authors.initials: X
authors.lastname: Jiang
authors.forename: Louise M
authors.initials: LM
authors.lastname: Garone
authors.forename: William H
authors.initials: WH
authors.lastname: Brondyk
mesh_terms: D000373:Agglutinins / Q000235:genetics; D019921:Biotinylation; D002460:Cell Line; D003545:Cysteine / Q000737:chemistry; D004220:Disulfides / Q000737:chemistry; D015870:Gene Expression; D015723:Gene Library; D006026:Glycoside Hydrolases / Q000235:genetics; D006801:Humans; D008958:Models, Molecular; D015394:Molecular Structure; D016296:Mutagenesis; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011392:Proline; D011487:Protein Conformation; D017510:Protein Folding; D020836:Protein Structure, Quaternary; D012333:RNA, Messenger / Q000032:analysis; D018124:Receptors, Tumor Necrosis Factor / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013329:Structure-Activity Relationship; D020349:Surface Plasmon Resonance; D013816:Thermodynamics; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D043324:beta-Fructofuranosidase
chemical_list: D000373:Agglutinins; D004220:Disulfides; D010446:Peptide Fragments; D012333:RNA, Messenger; D018124:Receptors, Tumor Necrosis Factor; D011993:Recombinant Fusion Proteins; D014409:Tumor Necrosis Factor-alpha; D011392:Proline; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase; D003545:Cysteine