Large-volume leukapheresis using femoral venous access for harvesting peripheral blood stem cells with the Fenwal CS 3000 Plus from normal healthy donors: predictors of CD34+ cell yield and collection efficiency.
The current paper reports on the predicting factors associated with satisfactory peripheral blood stem cell collection and the efficacy of large-volume leukapheresis (LVL) using femoral vein catheterization to harvest PBSCs with Fenwal CS 3000 Plus from normal healthy donors for allogeneic transplantation. A total of 113 apheresis procedures in 57 patients were performed. The median number of MNCs, CD3+ cells, and CD34+ cells harvested per apheresis was 5.3 x 10(8)/kg (range, 0.3-11.0 x 10(8)/kg), 3.0 x 10(8)/kg (range, 0.2-6.6 x 10(8)/kg), and 7.9 x 10(6)/kg (range, 0.1-188.9 x 10(6)/kg), respectively. The median collection efficiency of MNCs and CD34+ cells was 49.8% and 49.7%, respectively. A highly significant correlation was found between the collected CD34+ cell counts and the pre-apheresis WBC counts in the donors (P = 0.013), and between the collected CD34+ cell counts and the pre-apheresis peripheral blood (PB) CD34+ cell counts (P<0.001). Harvesting at least >4 x 10(6)/kg CD34+ cells from the 1st LVL was achieved in 44 (77.2%) out of 57 donors and in 19 (90.5%) out of 21 donors with a PB-CD34+ cell count of >40/microl. There was no significant difference in the harvested MNC and CD34+ cell counts between the 1st and 2nd apheresis. The catheter-related complications included catheter obstruction (n = 2) and hematoma at the insertion site (n = 3). Accordingly, LVL using femoral venous access for allogeneic PBSC collection from normal healthy donors would appear to be safe and effective.
Sohn
Sang Kyun
SK
Department of Hematology/Oncology, Kyungpook National University Hospital, Taegu, South Korea. sksohn@knu.ac.kr
Kim
Jong Gwang
JG
Chae
Yeo Soo
YS
Kim
Dong Hwan
DH
Lee
Nan Young
NY
Suh
Jang Soo
JS
Lee
Kyu Bo
KB
D000293:Adolescent; D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000032:analysis; D001782:Blood Donors; D017252:CD3 Complex / Q000032:analysis; D002408:Catheters, Indwelling / Q000009:adverse effects; D005260:Female; D005268:Femoral Vein; D006412:Hematopoietic Stem Cells; D006801:Humans; D007937:Leukapheresis / Q000295:instrumentation* / Q000379:methods* / Q000592:standards; D007958:Leukocyte Count; D007963:Leukocytes, Mononuclear; D008297:Male; D014184:Transplantation, Homologous
D018952:Antigens, CD34; D017252:CD3 Complex