Targeted recruitment of a histone H4-specific methyltransferase by the transcription factor YY1.
Methylation of specific residues within the N-terminal histone tails plays a critical role in regulating eukaryotic gene expression. Although great advances have been made toward identifying histone methyltransferases (HMTs) and elucidating the consequences of histone methylation, little is known about the recruitment of HMTs to regulatory regions of chromatin. Here we report that the sequence-specific DNA-binding transcription factor Yin Yang 1 (YY1) binds to and recruits the histone H4 (Arg 3)-specific methyltransferase, PRMT1, to a YY1-activated promoter. Our data confirm that histone methylation does not occur randomly but rather is a targeted event and provides one mechanism by which HMTs can be recruited to chromatin to activate gene expression.
Rezai-Zadeh
Natalie
N
H. Lee Moffitt Cancer Center & Research Institute, University of South Florida, Tampa 33612, USA.
Zhang
Xiaohong
X
Namour
Fares
F
Fejer
Gyorgy
G
Wen
Yu-Der
YD
Yao
Ya-Li
YL
Gyory
Ildiko
I
Wright
Kenneth
K
Seto
Edward
E
D000595:Amino Acid Sequence; D000818:Animals; D001120:Arginine / Q000378:metabolism*; D001665:Binding Sites; D015153:Blotting, Western; D002843:Chromatin / Q000235:genetics; D017931:DNA Primers / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D050981:Erythroid-Specific DNA-Binding Factors; D005786:Gene Expression Regulation; D005982:Glutathione Transferase / Q000737:chemistry / Q000378:metabolism; D006657:Histones / Q000378:metabolism*; D008156:Luciferases / Q000378:metabolism; D008745:Methylation; D008969:Molecular Sequence Data; D050779:Nuclear Factor 90 Proteins; D010750:Phosphoproteins; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011484:Protein-Arginine N-Methyltransferases / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D012097:Repressor Proteins; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D014162:Transfection; D050986:YY1 Transcription Factor; D016335:Zinc Fingers
D002843:Chromatin; D017931:DNA Primers; D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D006657:Histones; C429643:ILF3 protein, human; D050779:Nuclear Factor 90 Proteins; D010750:Phosphoproteins; D016601:RNA-Binding Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; D050986:YY1 Transcription Factor; D001120:Arginine; D008156:Luciferases; D011484:Protein-Arginine N-Methyltransferases; D005982:Glutathione Transferase