Monitoring the persistence of genes deriving from genetically modified plants in the soil environment.
To study the gene persistence in the soil environment, soil samples were collected from sugar beet (Beta vulgaris) and chicory (Cichorium intybus) experimental fields just before harvest. They were homogenized, mixed and stored at constant humidity in a non-heated room. Sub-samples of soils were subsequently collected at regular intervals, dried and sieved through a 1.8-mm mesh before DNA was extracted. Specific primers were then used for the detection of plant DNA by hot start PCR. Results reveal that, under laboratory conditions, transgenic and non-transgenic sugar beet DNA was still detected after 25 days incubation in the soil taken from a sugar beet experimental plot while detection of chicory DNA was still possible after 50 days incubation in soil taken from the chicory experimental plot. This might be in correlation with the stronger resistance of chicory radicles to decomposition as compared to radicles from sugar beets.
Degand
I
I
VAR, Veterinary and Agrochemical Research Centre, Leuvensesteenweg, 17, 3080 Tervuren, Belgium.
Laporte
J
J
Pussemier
L
L
D027461:Beta vulgaris / Q000235:genetics / Q000254:growth & development; D018651:Cichorium intybus / Q000235:genetics / Q000254:growth & development; D018744:DNA, Plant / Q000235:genetics / Q000378:metabolism; D063245:Plant Development; D010944:Plants / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000254:growth & development; D016133:Polymerase Chain Reaction / Q000379:methods; D012987:Soil / Q000032:analysis*; D013045:Species Specificity
D018744:DNA, Plant; D012987:Soil