title: In vitro methods for culturing vertebrate and mosquito stages of Plasmodium.
abstract: The development of in vitro culture systems for the vertebrate stages of Plasmodium led to major advancements in malaria research. Here we review both improvements made in these techniques and the recent achievement of the in vitro growth of mosquito stages from ookinete to infective sporozoite.
authors.affiliation: Centre for Applied Entomology and Parasitology, School of Biological Sciences, Keele University, Staffordshire ST5 5BG, UK. h.hurd@keele.ac.uk
authors.forename: Hilary
authors.initials: H
authors.lastname: Hurd
authors.forename: Ebtesam
authors.initials: E
authors.lastname: Al-Olayan
authors.forename: Geoffrey A
authors.initials: GA
authors.lastname: Butcher
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D009033:Culicidae / Q000469:parasitology; D003470:Culture Media; D004912:Erythrocytes / Q000469:parasitology; D006801:Humans; D007303:Insect Vectors / Q000469:parasitology; D008018:Life Cycle Stages; D010274:Parasitology / Q000379:methods*; D010961:Plasmodium / Q000254:growth & development*; D012100:Reproduction, Asexual; D034101:Sporozoites / Q000254:growth & development; D014714:Vertebrates / Q000469:parasitology*
chemical_list: D003470:Culture Media