title: Stress regulates the lymphocyte homing receptor CD62L (L-selectin).
abstract: Based on a previous study showing that panic disorder patients had increased expression of na ve phenotype lymphocytes (CD45RA+ and CD62L+), increased plasma cortisol, as well as decreased interleukin-2 (IL-2) producion, we hypothesized that changes in the percentage of expression of these lymphocyte surface molecules could be related to the substances released by the hypothalamic-pituitary-adrenal (HPA) axis and possibly associated to panic disorder (cortisol, IL-2, serotonin and epinephrine). In order to study the altered expression, blood mononuclear cells of normal volunteers were stimulated with mitogen, in the presence of dexamethasone, IL-2, serotonin and epinephrin. CD62L is decreased by IL-2 in vitro. Serotonin and epinephrine did not promote changes in the expression of these surface molecules. The results of the ex vivo study are in agreement with a previous clinical study with panic patients. It could be suggested that stress is responsible for certain immunologic dysfunctions and new studies should be conducted.
authors.affiliation: Departamento de Bioquímica, Universidade Federal do Rio Grande do Sul, Serviço de Psiquiatria, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil. gmanfro@pportoweb.com.br
authors.forename: Gisele Gus
authors.initials: GG
authors.lastname: Manfro
authors.forename: Carlos
authors.initials: C
authors.lastname: Alexandre Netto
authors.forename: Mark
authors.initials: M
authors.lastname: Pollack
authors.forename: Kelin Maggioni
authors.initials: KM
authors.lastname: Mezzomo
authors.forename: Frederic
authors.initials: F
authors.lastname: Preffer
authors.forename: Richard
authors.initials: R
authors.lastname: Kradin
mesh_terms: D000704:Analysis of Variance; D000893:Anti-Inflammatory Agents / Q000097:blood / Q000494:pharmacology; D015415:Biomarkers; D016022:Case-Control Studies; D015815:Cell Adhesion Molecules / Q000276:immunology; D002478:Cells, Cultured / Q000187:drug effects; D003907:Dexamethasone / Q000097:blood / Q000494:pharmacology; D004837:Epinephrine / Q000097:blood*; D005786:Gene Expression Regulation; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D019041:L-Selectin / Q000097:blood*; D018655:Lymphocyte Count; D016584:Panic Disorder / Q000097:blood / Q000276:immunology* / Q000523:psychology; D012701:Serotonin / Q000097:blood; D013315:Stress, Psychological / Q000097:blood / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000893:Anti-Inflammatory Agents; D015415:Biomarkers; D015815:Cell Adhesion Molecules; D007376:Interleukin-2; D019041:L-Selectin; D012701:Serotonin; D003907:Dexamethasone; D004837:Epinephrine