Real-time PCR quantitation of FE65 a beta-amyloid precursor protein-binding protein after traumatic brain injury in rats.
In cases of traumatic brain injury (TBI) in which the patient survived for only a short period of time and was without macroscopic changes at autopsy, it is difficult to diagnose TBI. To detect early diagnostic markers of diffuse axonal injury (DAI), real-time quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) in an experimental head trauma model of rat was chosen. The beta-amyloid precursor protein (beta-APP) is a well-known diagnostic marker of DAI which can be detected by immunolabeling as early as 1.5 h after injury. beta-APP has a binding protein, FE65, which is expressed in the brain of Alzheimer's disease patients along with beta-APP, but no involvement with brain injury has been reported. Neuron-specific enolase (NSE) is also a useful marker of DAI. We found that FE65 expression increased dramatically as early as 30 min after injury and decreased after peaking 1 h post-injury, although NSE showed no significant changes. These results suggest that real-time PCR of FE65 mRNA is useful for the diagnosis of DAI in forensic cases.
Iino
Morio
M
Department of Legal Medicine, Osaka University Graduate School of Medicine, 2-2-F3 Yamada-oka, Suita, 565-0871 Osaka, Japan. iino@legal.med.osaka-u.ac.jp
Nakatome
Masato
M
Ogura
Yoshiaki
Y
Fujimura
Harutoshi
H
Kuroki
Hisanaga
H
Inoue
Hiromasa
H
Ino
Yukiko
Y
Fujii
Tasuku
T
Terao
Toshiyuki
T
Matoba
Ryoji
R
D016564:Amyloid beta-Protein Precursor / Q000378:metabolism*; D000818:Animals; D001344:Autopsy; D015415:Biomarkers / Q000032:analysis; D020833:Diffuse Axonal Injury / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008297:Male; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000096:biosynthesis; D009687:Nuclear Proteins / Q000032:analysis* / Q000096:biosynthesis; D011485:Protein Binding; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
C091042:APBB1 protein, human; D016564:Amyloid beta-Protein Precursor; C486242:Apbb1 protein, rat; D015415:Biomarkers; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger