Epinephrine modulates cellular distribution of muscle phosphofructokinase.
In this paper, we report evidences that cellular distribution of phosphofructokinase can be affected by epinephrine stimulation in rabbit skeletal muscle homogenates. Through co-sedimentation techniques, we observed that in epinephrine-stimulated tissues, approximately 50% of phosphofructokinase activity is co-located in an actin-enriched fraction, against 29% in control. This phenomenon is accompanied by a 400% increase in specific phosphofructokinase activity in stimulated homogenates. This effect is reproduced by the beta-adrenergic agonist isoprenaline. Here we propose that the modulation of cellular distribution of phosphofructokinase may be one of the mechanisms of control of glycolytic flux in mammalian muscle, by beta-adrenergic stimulation.
Gomes Alves
Gutemberg
G
Laboratório de Enzimologia e Controle do Metabolismo (LabECoM), Departamento de Fármacos, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Sola-Penna
Mauro
M
D000199:Actins / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D004837:Epinephrine / Q000378:metabolism / Q000494:pharmacology*; D007545:Isoproterenol / Q000494:pharmacology; D018482:Muscle, Skeletal / Q000378:metabolism; D009132:Muscles / Q000201:enzymology*; D010732:Phosphofructokinase-1 / Q000096:biosynthesis* / Q000737:chemistry* / Q000378:metabolism; D011817:Rabbits; D013347:Subcellular Fractions; D014018:Tissue Distribution
D000199:Actins; D010732:Phosphofructokinase-1; D007545:Isoproterenol; D004837:Epinephrine