title: Physiological behaviour of Hanseniaspora guilliermondii in aerobic glucose-limited continuous cultures.
abstract: The physiology of Hanseniaspora guilliermondii was studied under aerobic glucose-limited conditions using the accelerostat procedure (continuous acceleration of dilution rate) and classical chemostat cultures. By both cultivation techniques this yeast was found to be Crabtree-positive. Up to a dilution rate of 0.25 h(-1), glucose was completely metabolised into biomass, glycerol and carbon dioxide. Above this value, an increase in the dilution rate was accompanied by the production of other metabolites like ethanol, acetic and malic acids. Biomass yield during the purely oxidative growth was 0.49 g g(-1) and decreased to 0.26 g g(-1) for D=0.34 h(-1). A maximal specific ethanol production rate of 1.36 mmol g(-1) h(-1) and a maximal ethanol yield of 0.05 g g(-1) were achieved at D=0.34 h(-1).
authors.affiliation: Unidade de Fisiologia Microbiana e Bioprocessos, Departamento Biotecnologia, Instituto Nacional de Engenharia e Tecnologia Industrial, Estrada do Paço do Lumiar, 22, 1649-038 Lisbon, Portugal.
authors.forename: Helena
authors.initials: H
authors.lastname: Albergaria
authors.forename: Ana R
authors.initials: AR
authors.lastname: Torrão
authors.forename: Timothy
authors.initials: T
authors.lastname: Hogg
authors.forename: Francisco M
authors.initials: FM
authors.lastname: Gírio
mesh_terms: D000332:Aerobiosis; D018533:Biomass; D019149:Bioreactors; D002245:Carbon Dioxide / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism; D010101:Oxygen Consumption / Q000502:physiology; D004718:Saccharomycetales / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D027843:Vitis / Q000382:microbiology
chemical_list: D002245:Carbon Dioxide; D000431:Ethanol; D005947:Glucose; D005990:Glycerol