title: Treponema socranskii in primary endodontic infections as detected by nested PCR.
abstract: Spirochetes have been frequently observed in root canal infections, but they were rarely identified. The purpose of this study was to investigate the prevalence of Treponema socranskii in primary endodontic infections using a species-specific nested polymerase chain reaction assay. Samples were collected from 60 teeth having carious lesions, necrotic pulps, and different forms of periradicular diseases. DNA extracted from the samples was initially amplified using universal 16S rDNA primers. A second round of amplification used the first polymerase chain reaction products to detect a specific fragment of T. socranskii 16S rDNA. T. socranskii was detected in 11 of 28 asymptomatic cases (39.3%), five of 12 root canals associated with acute apical periodontitis (41.7%), and five of 20 cases diagnosed as acute periradicular abscesses (25%). There was no relationship between the presence of T. socranskii and the occurrence of symptoms. In general, this spirochete was detected in 21 of 60 samples of endodontic infections (35%). Findings suggest that T. socranskii can be involved in the pathogenesis of different forms of periradicular lesions.
authors.affiliation: Department of Endodontics, Faculty of Dentistry, Estácio de Sá University, Rio de Janeiro, Brazil.
authors.forename: José F
authors.initials: JF
authors.lastname: Siqueira
authors.forename: Isabela N
authors.initials: IN
authors.lastname: Rôças
mesh_terms: D000293:Adolescent; D000328:Adult; D016009:Chi-Square Distribution; D004269:DNA, Bacterial / Q000032:analysis; D003790:Dental Pulp Necrosis / Q000382:microbiology*; D006801:Humans; D008875:Middle Aged; D010485:Periapical Periodontitis / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D014208:Treponema / Q000302:isolation & purification; D014211:Treponemal Infections / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial