title: The recX gene product is involved in the SOS response in Herbaspirillum seropedicae.
abstract: The recA and the recX genes of Herbaspirillum seropedicae were sequenced. The recX is located 359 bp downstream from recA. Sequence analysis indicated the presence of a putative operator site overlapping a probable sigma70-dependent promoter upstream of recA and a transcription terminator downstream from recX, with no apparent promoter sequence in the intergenic region. Transcriptional analysis using lacZ promoter fusions indicated that recA expression increased three- to fourfold in the presence of methyl methanesulfonate (MMS). The roles of recA and recX genes in the SOS response were determined from studies of chromosomal mutants. The recA mutant showed the highest sensitivity to MMS and UV, and the recX mutant had an intermediate sensitivity, compared with the wild type (SMR1), confirming the essential role of the RecA protein in cell viability in the presence of mutagenic agents and also indicating a role for RecX in the SOS response.
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal do Parana, C.P. 19046, 81531-990, Curitiba, PR, Brazil.
authors.forename: Carolina W
authors.initials: CW
authors.lastname: Galvão
authors.forename: Fábio O
authors.initials: FO
authors.lastname: Pedrosa
authors.forename: Emanuel M
authors.initials: EM
authors.lastname: Souza
authors.forename: M Geoffrey
authors.initials: MG
authors.lastname: Yates
authors.forename: Leda S
authors.initials: LS
authors.lastname: Chubatsu
authors.forename: Maria Berenice R
authors.initials: MB
authors.lastname: Steffens
mesh_terms: D001426:Bacterial Proteins / Q000502:physiology*; D020562:Betaproteobacteria / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D015169:Colony Count, Microbial; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D008957:Models, Genetic; D009586:Nitrogen Fixation; D013014:SOS Response, Genetics / Q000235:genetics / Q000502:physiology*; D014466:Ultraviolet Rays
chemical_list: D001426:Bacterial Proteins; C430523:RecX protein, Xanthomonas campestris; D008741:Methyl Methanesulfonate