Expression of HMGI(Y) associated with malignant phenotype of human gastric tissue.
OBJECTIVE
We evaluated the association between HMGI(Y) expression and the detection of malignant cells by simple reverse transcriptase-polymerase chain reaction (RT-PCR) and correlated the level of HMGI(Y) expression and the clinicopathological data in gastric cancer.


RESULTS
We analysed HMGI(Y) expression in 62 gastric cancer tissues and 28 normal gastric tissues by RT-PCR and immunohistochemical study. HMGI(Y) expression evidenced by RT-PCR was observed in 42 (67.7%) of 62 gastric cancer samples, whereas eight (28.6%) of 28 normal gastric tissues were positive (P = 0.001). In immunohistochemical staining for HMGI(Y), 48 (77.4%) of 62 gastric cancers were positive for HMGI(Y), whereas four (6.5%) of 62 normal gastric mucosae around the tumour were weakly positive, particularly in cells of some hyperplastic glands (P < 0.001). There was no significant correlation between the levels of HMGI(Y) expression and stage, tumour size, histological grade, invasion depth, or lymph node metastasis.


CONCLUSIONS
Our results suggest that HMGI(Y) expression could be associated with malignant phenotype in human gastric tissue, and that HMGI(Y) may contribute to gastric tumorigenesis.
Nam
E S
ES
Department of Pathology, Hallym University, Seoul, Korea.
Kim
D H
DH
Cho
S J
SJ
Chae
S W
SW
Kim
H Y
HY
Kim
S M
SM
Han
J J
JJ
Shin
H S
HS
Park
Y E
YE
D000230:Adenocarcinoma / Q000378:metabolism* / Q000556:secondary; D005753:Gastric Mucosa / Q000378:metabolism*; D025741:HMGA1a Protein / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D010641:Phenotype; D012333:RNA, Messenger / Q000378:metabolism; D012334:RNA, Neoplasm / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013270:Stomach / Q000473:pathology; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology
D012333:RNA, Messenger; D012334:RNA, Neoplasm; D025741:HMGA1a Protein