title: Isoprenoid biosynthesis via the methylerythritol phosphate pathway: the (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase (LytB/IspH) from Escherichia coli is a [4Fe-4S] protein.
abstract: The last enzyme (LytB) of the methylerythritol phosphate pathway for isoprenoid biosynthesis catalyzes the reduction of (E)-4-hydroxy-3-methylbut-2-enyl diphosphate into isopentenyl diphosphate and dimethylallyl diphosphate. This enzyme possesses a dioxygen-sensitive [4Fe-4S] cluster. This prosthetic group was characterized in the Escherichia coli enzyme by UV/visible and electron paramagnetic resonance spectroscopy after reconstitution of the purified protein. Enzymatic activity required the presence of a reducing system such as flavodoxin/flavodoxin reductase/reduced nicotinamide adenine dinucleotide phosphate or the photoreduced deazaflavin radical.
authors.affiliation: Université Louis Pasteur, UMR CNRS 7123, Institut Le Bel, Strasbourg, France.
authors.forename: Murielle
authors.initials: M
authors.lastname: Wolff
authors.forename: Myriam
authors.initials: M
authors.lastname: Seemann
authors.forename: Bernadette
authors.initials: B
authors.lastname: Tse Sum Bui
authors.forename: Yves
authors.initials: Y
authors.lastname: Frapart
authors.forename: Denis
authors.initials: D
authors.lastname: Tritsch
authors.forename: Ana
authors.initials: A
authors.lastname: Garcia Estrabot
authors.forename: Manuel
authors.initials: M
authors.lastname: Rodríguez-Concepción
authors.forename: Albert
authors.initials: A
authors.lastname: Boronat
authors.forename: Andrée
authors.initials: A
authors.lastname: Marquet
authors.forename: Michel
authors.initials: M
authors.lastname: Rohmer
mesh_terms: D011756:Diphosphates / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D004896:Erythritol / Q000031:analogs & derivatives* / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000378:metabolism*; D045782:Hemiterpenes; D007506:Iron-Sulfur Proteins / Q000737:chemistry / Q000378:metabolism*; D008956:Models, Chemical; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism*; D010088:Oxidoreductases / Q000737:chemistry / Q000378:metabolism*; D011105:Polyisoprenyl Phosphate Sugars / Q000096:biosynthesis*; D013403:Sugar Phosphates / Q000378:metabolism*
chemical_list: C114232:2-C-methylerythritol 4-phosphate; C443995:4-hydroxy-3-methylbut-2-enyl pyrophosphate; D011756:Diphosphates; D029968:Escherichia coli Proteins; D045782:Hemiterpenes; D007506:Iron-Sulfur Proteins; D009943:Organophosphorus Compounds; D011105:Polyisoprenyl Phosphate Sugars; D013403:Sugar Phosphates; C004809:isopentenyl pyrophosphate; D010088:Oxidoreductases; C109866:ispH protein, E coli; D009247:NADH, NADPH Oxidoreductases; C116041:flavodoxin NADPH oxidoreductase; D004896:Erythritol