Electrical and dielectric properties of bovine trabecular bone--relationships with mechanical properties and mineral density.
Interrelationships of trabecular bone electrical and dielectric properties with mechanical characteristics and density are poorly known. While electrical stimulation is used for healing fractures, better understanding of these relations has clinical importance. Furthermore, earlier studies have suggested that bone electrical and dielectric properties depend on the bone density and could, therefore, be used to predict bone strength. To clarify these issues, volumetric bone mineral density (BMDvol), electrical and dielectric as well as mechanical properties were determined from 40 cylindrical plugs of bovine trabecular bone. Phase angle, relative permittivity, loss factor and conductivity of wet bovine trabecular bone were correlated with Young's modulus, yield stress, ultimate strength, resilience and BMDvol. The reproducibility of in vitro electrical and dielectric measurements was excellent (standardized coefficient of variation less than 1%, for all parameters), especially at frequencies higher than 1 kHz. Correlations of electrical and dielectric parameters with the bone mechanical properties or density were frequency-dependent. The relative permittivity showed the strongest linear correlations with mechanical parameters (r > 0.547, p < 0.01, n = 40, at 50 kHz) and with BMDvol (r = 0.866, p < 0.01, n = 40, at 50 kHz). In general, linear correlations between relative permittivity and mechanical properties or BMDvol were highest at frequencies over 6 kHz. In addition, a significant site-dependent variation of electrical and dielectric characteristics, mechanical properties and BMDvol was revealed in bovine femur (p < 0.05, Kruskall-Wallis H-test). Based on the present results, we conclude that the measurement of electrical and dielectric properties provides quantitative information that is related to bone quantity and quality.
Sierpowska
J
J
Department of Applied Physics, University of Kuopio, POB 1627, 70211 Kuopio, Finland. joanna.sierpowska@uku.fi
Töyräs
J
J
Hakulinen
M A
MA
Saarakkala
S
S
Jurvelin
J S
JS
Lappalainen
R
R
D000818:Animals; D015519:Bone Density / Q000502:physiology*; D002102:Cadaver; D002417:Cattle; D019245:Compressive Strength; D004548:Elasticity; D004553:Electric Conductivity; D017097:Electric Impedance; D005269:Femur / Q000502:physiology*; D066298:In Vitro Techniques; D009038:Motion; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013223:Statistics as Topic; D013314:Stress, Mechanical; D016474:Weight-Bearing / Q000502:physiology