Identification of genes down-regulated during melanoma progression: a cDNA array study.
In order to identify genes relevant for melanoma development, we carried out cDNA array experiments employing an in vitro model of human melanoma progression, consisting of two cell lines: one, LP, derived from a primary melanoma and the other, LM, from its metastatic supraclavicular lymph node. Basic cDNA array data identified 26 genes as down-regulated in the LM cell line. Northern blot analysis confirmed an effective transcriptional down-regulation for five out of 13 genes analyzed. The products of these five genes belong to different functional protein types, such as transcription and translation regulators (Edg-2, eIF-3 p110, and RNPL/RBM3), extracellular communicators (PRSS11) and members of the major histocompatibility complex (beta2-microglobulin). Some previously described differences in expression patterns, such as loss of HLA I, were confirmed by our array data. In addition, we identified and validated for the first time the reduced expression level of several genes during melanoma progression. In particular, reduced Edg-2 gene product expression was also confirmed in a group of 50 primary melanomas and unrelated metastases. In conclusion, comparative hybridization by means of cDNA arrays assisted in identifying a series of novel progression-associated changes in gene expression, confirming, at the same time, a number of previously described results.
Baldi
Alfonso
A
Laboratory C, Department for the Development of Therapeutic Programs, Center for Experimental Research, Regina Elena Cancer Institute, Rome, Italy.
Battista
Tullio
T
De Luca
Antonio
A
Santini
Daniele
D
Rossiello
Luigi
L
Baldi
Feliciano
F
Natali
Pier Giorgio
PG
Lombardi
Daniela
D
Picardo
Mauro
M
Felsani
Armando
A
Paggi
Marco G
MG
D045744:Cell Line, Tumor; D015536:Down-Regulation; D020869:Gene Expression Profiling; D005805:Genes, MHC Class I; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis / Q000235:genetics; D008545:Melanoma / Q000209:etiology / Q000235:genetics* / Q000378:metabolism / Q000556:secondary; D020411:Oligonucleotide Array Sequence Analysis; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism; D049368:Receptors, Lysophosphatidic Acid; D001613:beta 2-Microglobulin / Q000235:genetics
D043562:Receptors, G-Protein-Coupled; D049368:Receptors, Lysophosphatidic Acid; D001613:beta 2-Microglobulin