title: Flavobacterium limicola sp. nov., a psychrophilic, organic-polymer-degrading bacterium isolated from freshwater sediments.
abstract: Three novel strains of cold-adapted bacteria, ST-82T, ST-10 and ST-92, were isolated from freshwater sediments. These three isolates were very similar to each other in phenotypic and chemotaxonomic traits, as well as in 16S rDNA sequence. The strains were Gram-negative, elongated filament-like rods that formed bright yellow colonies. They showed neither flexirubin pigments nor gliding motility. The strains were able to hydrolyse casein, gelatin, starch, agar, aesculin, urea, uric acid and tyrosine. They also lysed cells of Escherichia coil and Pseudomonas putida. The temperature range for growth was 0-25 degrees C, with optimum growth occurring at 15-20 degrees C. For all isolates, protease secretion increased as temperature decreased. Sodium chloride inhibited their growth, although the strains tolerated up to 1.5% (w/v) NaCl. Menaquinone-6 was the major respiratory quinone. The major cellular fatty acids were C15 : 0, iso-C15 : 0, anteiso-C15 : 0, C15:1, iso-C15:1, C16 : 1omega7cis, iso-C16 : 1, iso-C17 : 1, iso-C15 : 3-OH and iso-C16 : 0 3-OH. The DNA G + C content was 34.0-34.8 mol%. Phylogenetic analysis based on 16S rDNA sequences suggested that the strains belonged to the genus Flavobacterium and were closely related to Flavobacterium xanthum and Flavobacterium frigidarium, with sequence similarities of 96.9 and 96.3%, respectively. In physiological and biochemical analyses, the isolates were differentiated from all known members of the genus Flavobacterium. The name Flavobacterium limicola is proposed for these novel strains, and the type strain is ST-82T (=JCM 11473T =DSM 15094T).
authors.affiliation: Research Institute of Biological Resources, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.|Institute of Applied Biochemistry, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Tamaki
authors.affiliation: Research Institute of Biological Resources, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hanada
authors.affiliation: Research Institute of Biological Resources, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Kamagata
authors.affiliation: Research Institute of Biological Resources, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Nakamura
authors.affiliation: Science and Technology Promotion Foundation of Ibaraki, 2-1-6 Sengen, Tsukuba, Ibaraki 305-0047, Japan.
authors.forename: Nakao
authors.initials: N
authors.lastname: Nomura
authors.affiliation: Institute of Applied Biochemistry, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Nakano
authors.affiliation: Institute of Applied Biochemistry, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan.
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Matsumura
mesh_terms: D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis*; D005417:Flavobacterium / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology; D005618:Fresh Water / Q000382:microbiology*; D019015:Geologic Sediments; D008858:Microscopy, Phase-Contrast; D008969:Molecular Sequence Data; D009994:Osmolar Concentration; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D017422:Sequence Analysis, DNA; D013696:Temperature; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S