Determination of vascular-endothelial growth factor levels in serum from tumor-bearing BALB/c mice treated with photodynamic therapy.
METHODS
We implanted a malignant tumour, BFS1 fibrosarcoma, into BALB/c mice and then treated them using a new photosensitizer, hydroxygallium (III) phthalocyanine tetrasulfonic acid tetrasodium salt, BON-6. The administration of this compound was followed by light irradiation using a halogen lamp at 680 nm. VEGF concentrations were measured in sera from the mice and compared to the time of tumor growth.


RESULTS
BON-6 was found to be effective in PDT. This feature was accompanied by low levels of VEGF after BON-6+PDT, and also prolongation of the time of survival of treated animals. The mice which received BON-6+PDT survived 83.8 days (SD 10.23). The mean survival time in control groups did not exceed 35 days. Additionally, measurement of tumor size showed total regression in single cases after BON-6+PDT.


CONCLUSIONS
PDT, by decreasing VEGF serum levels, may influence the capability of tumor tissue to form new vessels.
Osiecka
Beata J
BJ
Department of Pathology, Medical University, Wrocław, Poland.
Ziołkowski
Piotr
P
Gamian
Elzbieta
E
Lis-Nawara
Anna
A
White
Stephen G
SG
Bonnett
Raymond
R
D000818:Animals; D016228:Endothelial Growth Factors / Q000097:blood*; D005354:Fibrosarcoma / Q000097:blood / Q000188:drug therapy*; D036341:Intercellular Signaling Peptides and Proteins / Q000097:blood*; D008222:Lymphokines / Q000097:blood*; D051379:Mice; D008807:Mice, Inbred BALB C; D009389:Neovascularization, Pathologic / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D009942:Organometallic Compounds; D010778:Photochemotherapy; D015996:Survival Rate; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D016228:Endothelial Growth Factors; D036341:Intercellular Signaling Peptides and Proteins; D008222:Lymphokines; D009942:Organometallic Compounds; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; C475999:hydroxygallium (III) phthalocyanine tetrasulfonic acid tetrasodium salt