Vitamin D(3) up-regulating protein 1 (VDUP1) antisense DNA regulates tumorigenicity and melanogenesis of murine melanoma cells via regulating the expression of fas ligand and reactive oxygen species.
The expression of vitamin D(3) up-regulating protein-1 (VDUP1) was up-regulated by 1alpha,25-dihydroxyvitamin D(3) (VD3) treatment in B16 mouse melanoma cells. The functional effect of VDUP1 on B16F10 melanoma cells was demonstrated by reduction of Fas ligand and CD44 expression in cells transfected with VDUP1 antisense cDNA. Furthermore, intracellular reactive oxygen species level and cell proliferation were decreased in antisense transfectants compared with those in vector controls. However, melanin synthesis was up-regulated in antisense transfectants. In addition, VDUP1 antisense transfectants showed an increased susceptibility to natural killer (NK) cells in vitro. When VDUP1 antisense transfectants were implanted into syngeneic mice, significant reduction of tumor cell growth was observed with the infiltrate of T cells and NK cells in tumor area. Taken together, these results demonstrate that VDUP1 has critical physiological roles and can be a novel therapeutic target for melanoma.
Song
Hyunkeun
H
Laboratory of Immunology, Korea Research Institute of Bioscience and Biotechnology, Eoun-Dong 52, Yusong, Taejon 305-333, South Korea.
Cho
Daeho
D
Jeon
Jun Ho
JH
Han
Seung Hyun
SH
Hur
Dae Young
DY
Kim
Young Sang
YS
Choi
Inpyo
I
D000818:Animals; D002352:Carrier Proteins / Q000187:drug effects / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D045744:Cell Line, Tumor; D016373:DNA, Antisense; D053222:Fas Ligand Protein; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D018960:Hyaluronan Receptors / Q000096:biosynthesis; D015151:Immunoblotting; D007150:Immunohistochemistry; D007694:Killer Cells, Natural / Q000276:immunology; D008297:Male; D008543:Melanins / Q000096:biosynthesis; D008545:Melanoma / Q000276:immunology / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism*; D051379:Mice; D011073:Polyamines; D017382:Reactive Oxygen Species / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013879:Thioredoxins; D014162:Transfection; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000494:pharmacology
C115518:2,10-dioximino-3,9-dimethyl-4,8-diazaundeca-3,8-diene; D002352:Carrier Proteins; D016373:DNA, Antisense; C505661:FASLG protein, human; D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; D018960:Hyaluronan Receptors; D008543:Melanins; D008562:Membrane Glycoproteins; D011073:Polyamines; D017382:Reactive Oxygen Species; C486715:TXNIP protein, human; C118756:dihydroxy-vitamin D3; D014807:Vitamin D; D013879:Thioredoxins