title: Coupling capillary electrochromatography with electrospray Fourier transform mass spectrometry for characterizing complex oligosaccharide pools.
abstract: To deal with the complexity of the glycan mixtures released from glycoproteins, an efficient form of micro-column separations, capillary electrochromatography, has been combined with high-performance mass spectrometry (Fourier transform ion cyclotron resonance). Contour plots have been generated from the mixtures of O-linked oligosaccharides originated from bovine mucin and bile salt-stimulated lipase, a large glycoprotein enzyme.
authors.affiliation: Department of Chemistry, Indiana University, Bloomington, Indiana 47405, USA.
authors.forename: Amy H
authors.initials: AH
authors.lastname: Que
authors.forename: Yehia
authors.initials: Y
authors.lastname: Mechref
authors.forename: Yunping
authors.initials: Y
authors.lastname: Huang
authors.forename: John A
authors.initials: JA
authors.lastname: Taraszka
authors.forename: David E
authors.initials: DE
authors.lastname: Clemmer
authors.forename: Milos V
authors.initials: MV
authors.lastname: Novotny
mesh_terms: D000818:Animals; D002417:Cattle; D002845:Chromatography / Q000295:instrumentation / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D005583:Fourier Analysis; D006023:Glycoproteins / Q000737:chemistry*; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D009077:Mucins / Q000737:chemistry; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry*; D002787:Sterol Esterase / Q000737:chemistry
chemical_list: D006023:Glycoproteins; D009077:Mucins; D009844:Oligosaccharides; C054784:bile salt-stimulated lipase; D002787:Sterol Esterase