title: [Prokaryocytic expression of the shortened hepatitis B surface antigen and antigenic analysis].
abstract: OBJECTIVE
  To study the expression of the shortened hepatitis B surface antigen in prokaryocyte and detect the antigenic characters.
  
  
  METHODS
  Firstly, the gene fragments coding the 152 and 124 amino acids of the carboxyl terminus of hepatitis B surface antigen (HBsAg) were amplified by polymerase chain reaction (PCR). Secondly, they were cloned to plasmid pBKS+, and the accuracy of those constructions were confirmed by restriction enzyme digestion and DNA sequencing. Then, they were cloned to prokaryocytic expression vector-plasmid pET32a(+). The recombinant plasmids were transfected into E.coli BL21 and induced to express with IPTG.
  
  
  RESULTS
  The recombinant plasmids were successfully constructed. In E.coli BL21, the protein was expressed in a fusion fashion and could be recognized by monoclonal antibody against HBsAg with ELISA and Western blot.
  
  
  CONCLUSIONS
  The shortened HBsAg can be expressed in prokaryocyte.
authors.affiliation: Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Ling
authors.forename: Hong
authors.initials: H
authors.lastname: Ren
authors.forename: Ming-li
authors.initials: ML
authors.lastname: Peng
authors.forename: Hong-mei
authors.initials: HM
authors.lastname: Xu
authors.forename: Yu-ling
authors.initials: YL
authors.lastname: Qing
mesh_terms: D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology*; D006515:Hepatitis B virus / Q000235:genetics; D006801:Humans; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011387:Prokaryotic Cells / Q000378:metabolism; D011498:Protein Precursors / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology
chemical_list: D006514:Hepatitis B Surface Antigens; D011498:Protein Precursors; D011993:Recombinant Fusion Proteins; C050806:presurface protein 1, hepatitis B surface antigen; C050807:presurface protein 2, hepatitis B surface antigen