Use of high-performance liquid chromatography to study the pharmacokinetics of colistin sulfate in rats following intravenous administration.
The pharmacokinetics of colistin was investigated using specific high-performance liquid chromatography (HPLC) to measure the concentrations of colistin and colistin A and B in plasma and urine in five rats after administration of an intravenous bolus of 1 mg of colistin sulfate/kg of body weight. There were differences in the pharmacokinetic behaviors of unbound colistin A and B. This is the first report of the use of HPLC to study the pharmacokinetics of colistin and its two major components.
Li
Jian
J
Centre for Pharmaceutical Research. School of Pharmaceutical, Molecular and Biomedical Sciences, University of South Australia, Adelaide, Australia.
Milne
Robert W
RW
Nation
Roger L
RL
Turnidge
John D
JD
Smeaton
Timothy C
TC
Coulthard
Kingsley
K
D000818:Animals; D000900:Anti-Bacterial Agents / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid; D003091:Colistin / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics*; D007275:Injections, Intravenous; D008297:Male; D008657:Metabolic Clearance Rate; D011485:Protein Binding; D051381:Rats; D017207:Rats, Sprague-Dawley
D000900:Anti-Bacterial Agents; D003091:Colistin