The Candida albicans UBI3 gene encoding a hybrid ubiquitin fusion protein involved in ribosome biogenesis is essential for growth.
We have constructed a conditional null mutant Candida albicans strain for the UBI3 gene which encodes a ubiquitin fusion protein involved in ribosome biogenesis. A one-step gene disruption procedure, using the plasmid pCaDis, was designed to place the second copy of the UBI3 gene under the control of the tightly regulated MET3 promoter in a C. albicans heterozygous strain (UBI3/Deltaubi3::hisG), previously isolated in the first step of the ura-blaster protocol. Analysis of the conditional null mutant in repressing and inducing conditions indicates that UBI3 is an essential gene whose expression is required for growth of C. albicans.
Roig
Patricia
P
Departament de Microbiologia i Ecologia, Facultat de Farmàcia, Universitat de València, Avgda. Vicent Andrés Estellés s/n, 46100 Burjassot, Spain.
Gozalbo
Daniel
D
D001483:Base Sequence; D002176:Candida albicans / Q000235:genetics* / Q000254:growth & development*; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics / Q000502:physiology*; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012269:Ribosomal Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D012270:Ribosomes / Q000502:physiology*; D014452:Ubiquitins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D012269:Ribosomal Proteins; D014452:Ubiquitins; C085995:ubi3 protein, Solanum tuberosum