The Stimulating Effect of the beta-Endorphin-Like Peptide Immunorphin on the Human T-Lymphoblastoid Cell Line Jurkat Is Mediated by a Non-Opioid Receptor for beta-Endorphin.
It was shown that beta-endorphin and the synthetic decapeptide SLTCLVKGFY that corresponds to the amino acid sequence 364-373 of the human IgG heavy chain (referred to as immunorphin) is able to stimulate growth of the human T-lymphoblastoid cell line Jurkat. The antagonist of opioid receptors naloxone did not inhibit the stimulating effect of the peptides. Studies on [(3)H]-immunorphin binding to Jurkat cell receptors have demonstrated that it binds with high affinity to naloxone-insensitive receptors (K(d) = 1.3 nM; n = 5.2 x 10(5)). Unlabeled beta-endorphin and the 6-10 fragment of immunorphin completely inhibited the labeled ligand specific binding to naloxone-insensitive receptors on T lymphocytes (K(i) = 1.4 x 10(-7) and 3.7 x 10(-5) M, respectively). Thus, beta-endorphin and immunorphin share the naloxone-insensitive receptors on human T-lymphoblastoid cell line Jurkat.
Krasnova
Svetlana B
SB
Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, Moscow Region, Russia.
Malkova
Natalia V
NV
Kovalitskaya
Yuliya A
YA
Zolotarev
Yuri A
YA
Zargarova
Tatyana A
TA
Kolobov
Alexander A
AA
Kampe-Nemm
Elena A
EA
Navolotskaya
Elena V
EV
Lipkin
Valery M
VM
D006801:Humans; D019169:Jurkat Cells; D009270:Naloxone / Q000494:pharmacology; D010455:Peptides; D011957:Receptors, Opioid / Q000737:chemistry; D001615:beta-Endorphin / Q000378:metabolism
D010455:Peptides; D011957:Receptors, Opioid; D009270:Naloxone; D001615:beta-Endorphin