title: A germin-like protein of wheat leaf apoplast inhibits serine proteases.
abstract: A protein resistant to heat and proteolysis that inhibits serine proteases was isolated from wheat leaf apoplasts. Based on trypsin inhibition, its more active form was a 66-69 kDa oligomer. It was dissociated in an 18-21 kDa monomer having an amino terminal sequence identical to the Box A of germins and germin-like proteins. Like these proteins, it was glycosylated and showed manganese superoxide dismutase activity. The monomer displayed three forms when examined by 2D western blot: two of 19 kDa, pI 5.8 and 6.2; and one of 21 kDa, pI 5.8. It was found that the protein controls serine protease activity in the apoplast of plants challenged with the fungus Septoria tritici.
authors.affiliation: Instituto de Investigaciones Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, CC 1245, B7600GTQ, Mar del Plata, Argentina. segarra@mdp.edu.ar
authors.forename: Carmen Inés
authors.initials: CI
authors.lastname: Segarra
authors.forename: Claudia Anahí
authors.initials: CA
authors.lastname: Casalongué
authors.forename: Marcela Lilian
authors.initials: ML
authors.lastname: Pinedo
authors.forename: Virginia Paola
authors.initials: VP
authors.lastname: Ronchi
authors.forename: Rubén Danilo
authors.initials: RD
authors.lastname: Conde
mesh_terms: D000595:Amino Acid Sequence; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000378:metabolism* / Q000494:pharmacology; D018515:Plant Leaves / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism* / Q000494:pharmacology; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000378:metabolism; D014908:Triticum / Q000378:metabolism*; D014357:Trypsin / Q000187:drug effects / Q000378:metabolism
chemical_list: D006023:Glycoproteins; D010940:Plant Proteins; D015842:Serine Proteinase Inhibitors; C058814:germin; D013482:Superoxide Dismutase; D014357:Trypsin