Aberrant processing of Fas transcripts in adult T-cell leukemia: a possible role in tumor cell survival.
In adult T-cell leukemia (ATL), tumor cells commonly express abundant membrane-bound Fas antigen. We reported a significant correlation between Fas expression status of ATL patients and their clinical outcome. In the current study, we analyzed the Fas cDNA sequence of the distinctive ATL cases that barely expressed mFas identified during the previous study. According to the results, changes in the Fas amino acid sequence were deduced in two of seven cases. Furthermore, we identified seven novel variants of Fas mRNA produced by alternative splicing. Our data indicates the diversity of Fas gene expression at a mRNA level in ATL.
Tawara
Masayuki
M
Department of Laboratory Medicine, Nagasaki University School of Medicine, Nagasaki, Japan.
Maeda
Takahiro
T
Yamada
Yasuaki
Y
Harasawa
Hitomi
H
Tsuruda
Kazuto
K
Sugahara
Kazuyuki
K
Moriuchi
Ryozo
R
Tomonaga
Masao
M
Kamihira
Shimeru
S
D017398:Alternative Splicing; D017209:Apoptosis; D001483:Base Sequence; D002470:Cell Survival; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D006801:Humans; D015458:Leukemia, T-Cell / Q000378:metabolism*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009154:Mutation; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D019014:fas Receptor / Q000378:metabolism*
D018076:DNA, Complementary; D012333:RNA, Messenger; D019014:fas Receptor