Gelatin-glutaraldehyde cross-linking on silicone rubber to increase endothelial cell adhesion and growth.
Silicone is a biomaterial that is widely used in many areas because of its high optical clarity, its durability, and the ease with which it can be cast. However, these advantages are counterbalanced by strong hydrophobicity. Gelatin cross-linking has been used as a hydrophilic coating on many biomaterials but not on silicone rubber. In this study, two gelatin glutaraldehyde (GA) cross-linking methods were used to coat a hydrophilic membrane on silicone rubber. In method I, gelatin and GA were mixed in three different proportions (64:1, 128:1, and 256:1) before coating. In method II, a newly formed 5% gelatin membrane was cross-linked with a 2.5% GA solution. All coatings were hydrophilic, as determined from the measurement of contact angle for a drop of water on the surface. Bovine coronary arterial endothelial cells were shown to grow well on the surface modified by method II at 72 h. In method I, the cells grew well for gelatin-GA proportions of 64:1 and 128:1 at 72 h. No cell attachment on untreated silicone rubber was observed by the third d of seeding. The results indicated that both methods of gelatin-GA cross-linking provided a hydrophilic surface on silicone for endothelial cell adhesion and growth in vitro.
Ai
Hua
H
Department of Biomedical Engineering & School of Biological Sciences, Louisiana Tech University, Ruston, Louisiana 71272, USA.
Mills
David K
DK
Jonathan
Alexander S
AS
Jones
Steven A
SA
D000818:Animals; D002417:Cattle; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division / Q000502:physiology; D003331:Coronary Vessels; D003432:Cross-Linking Reagents; D004730:Endothelium, Vascular / Q000166:cytology* / Q000502:physiology*; D005780:Gelatin; D005976:Glutaral; D012828:Silicones
D003432:Cross-Linking Reagents; D012828:Silicones; D005780:Gelatin; D005976:Glutaral