In vivo formation of salsolinol induced by high acetaldehyde concentration in rat striatum employing microdialysis.
OBJECTIVE
The in vivo formation of salsolinol (1-methyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquionoline), an endogeneous condensation product of dopamine (DA) with acetaldehyde (AcH), was examined following the administration of cyanamide (CY) plus ethanol (EtOH) using microdialysis-high-performance liquid chromatography with electrochemical detection.


METHODS
After the insertion of a microdialysis probe into the striatum, rats were treated with CY (a potent inhibitor of aldehyde dehydrogenase, 50 mg/kg), 4-methylpyrazole (4-MP, a strong inhibitor of alcohol dehydrogenase, 82 mg/kg), and CY + 4-MP, followed 1 h later by EtOH (1 g/kg), CY and 4-MP only by intraperitoneal administration.


RESULTS
In the CY + EtOH group, salsolinol was detected in striatal dialysates and high AcH concentrations were found in the blood. The time course of changes in salsolinol concentrations correlated with blood AcH concentrations. In the other experimental groups, salsolinol in the dialysates and high AcH concentrations in the blood were not detected.


CONCLUSIONS
These observations indicate that: (1) high AcH concentrations induce the formation of salsolinol in the rat striatum; (2) there is no effect of EtOH or AcH on striatal dialysate concentrations of DA and 5-hydroxytryptamine.
Jamal
Mostofa
M
Department of Forensic Medicine, Faculty of Medicine, Kagawa Medical University, 1750-1, Ikenobe, Miki, Kita, Kagawa 761-0793, Japan.
Ameno
Kiyoshi
K
Kubota
Takako
T
Ameno
Setsuko
S
Zhang
Xia
X
Kumihashi
Mitsuru
M
Ijiri
Iwao
I
D000079:Acetaldehyde / Q000097:blood / Q000494:pharmacology*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism; D003484:Cyanamide / Q000008:administration & dosage; D004298:Dopamine / Q000032:analysis / Q000378:metabolism*; D000431:Ethanol / Q000097:blood / Q000494:pharmacology*; D007546:Isoquinolines / Q000032:analysis / Q000378:metabolism*; D008297:Male; D017551:Microdialysis / Q000379:methods; D051381:Rats; D017208:Rats, Wistar
D007546:Isoquinolines; D000431:Ethanol; D003484:Cyanamide; C036617:salsolinol; D000079:Acetaldehyde; D004298:Dopamine