[Sequential changes of extracellular matrix metalloproteins in pregnancy and labor in the rat chorio-allantois].
Participation of matrix metalloproteinases (MMP) and their tissue inhibitors (TIMP) in the rupture of fetal membranes (FM) during labor has been proposed. We describe and characterize sequentially the activity of the enzymes that degrade connective tissue in the FM of the rat during pregnancy and labor. Pregnant Wistar rats were sacrificed on different days of gestation and samples of amniotic fluid (AF) and MCA were collected to be analyzed for proteolytic activity, zymography, western blot and northern blot. Results showed that during labor, there is a significant increase in the proteolytic activity in the MCA and in the AF. MMP-2 was identified from day 15 of pregnancy and it increased near labor. MMP-9 was only identified two days before labor. MMP-2 and MMP-9 mRNA expression were coincident with enzymatic activity and western blot data. During labor, TIMP-1 decreased and TIMP-2 did not change. These results allow us to conclude that there exists a quantitative and qualitative differential expression of MMPs during gestation, especially during labor.
Meraz-Cruz
Noemí
N
nmc@servidor.unam.mx
Molina Delgado
Guadalupe
G
Vadillo-Ortega
Felipe
F
D000482:Allantois / Q000201:enzymology*; D000818:Animals; D002823:Chorion / Q000201:enzymology*; D005260:Female; D007743:Labor, Obstetric / Q000378:metabolism*; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D020782:Matrix Metalloproteinases
Cambios secuenciales de metaloproteinasas de matriz extracelular durante la gestación y el trabajo de parto en el corioalantoides de la rata.