Direct electrochemistry of DNA, guanine and adenine at a nanostructured film-modified electrode.
A nanostructured film electrode, a multi-wall carbon nanotubes (MWNT)-modified glassy carbon electrode (GCE), is described for the simultaneous determination of guanine and adenine. The properties of the MWNT-modified GCE were investigated by scanning electron microscopy (SEM) and cyclic voltammetry. The oxidation peak currents of guanine and adenine increased significantly at the MWNT-modified GCE in contrast to those at the bare GCE. The experimental parameters were optimized and a direct electrochemical method for the simultaneous determination of guanine and adenine was proposed. Using the MWNT-modified GCE, a sensitive and direct electrochemical technique for the measurement of native DNA was also developed, and the value of (G+C)/(A+T) of HCl-digested DNA was detected.
Wu
Kangbing
K
Department of Chemistry, Wuhan University, 430072 Wuhan, P.R. China.
Fei
Junjie
J
Bai
Wen
W
Hu
Shengshui
S
D000225:Adenine / Q000032:analysis* / Q000737:chemistry; D000327:Adsorption; D000818:Animals; D002417:Cattle; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods*; D004566:Electrodes; D006147:Guanine / Q000032:analysis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009690:Nucleic Acid Conformation; D010755:Organophosphates; D012680:Sensitivity and Specificity
D010755:Organophosphates; C002290:dicetylphosphate; D006147:Guanine; D004247:DNA; D000225:Adenine