title: Laser Doppler imaging of burn scars: a comparison of wavelength and scanning methods.
abstract: Laser Doppler perfusion imaging (LDI) is a useful tool for the early clinical assessment of burn depth and prognostic evaluation of injuries that may require skin grafting. We have evaluated two commercially available laser Doppler imagers for the perfusion measurement of normal and burn scar tissue.
  
  
  METHODS
  A single wavelength (635 nm), step-wise scanning LDI and a dual wavelength (633 and 780 nm), continuous scanning LDI were used. Twenty patients with hypertrophic burn scars (time since injury: 1 month-8 years) were recruited and the color and elevation of the scar was clinically assessed using a modified Vancouver Burn Scar Scale. Perfusion of each scar region was measured using both imagers. A symmetric contralateral region of unburned skin was also imaged to record baseline perfusion.
  
  
  RESULTS
  Comparisons of wavelength and scanning technique were made using perfusion values obtained from 22 burn scars. Highly significant positive correlation was observed in all comparisons. In addition, output from both instruments was strongly and significantly correlated with the clinical grading of the scar.
  
  
  CONCLUSIONS
  Both LDI scanners perform similar perfusion measurements. The results also indicate that red and near-infrared (NIR) wavelength photons provide similar blood flow information. The faster, continuous scanning method provides a clinical advantage without a significant loss of blood flow information. However, a critical evaluation of both instruments suggests that caution must be exercised when using these optical diagnostic techniques and that some knowledge of light-tissue interaction is required for the proper analysis and interpretation of clinical data.
authors.affiliation: Department of Surgery, McCaig Center for Joint Injury and Arthritis Research, 3330 Hospital Dr. NW, University of Calgary, Calgary, Alta., Canada. rcbray@ucalgary.ca
authors.forename: Robert
authors.initials: R
authors.lastname: Bray
authors.forename: Kevin
authors.initials: K
authors.lastname: Forrester
authors.forename: Catherine
authors.initials: C
authors.lastname: Leonard
authors.forename: Ross
authors.initials: R
authors.lastname: McArthur
authors.forename: John
authors.initials: J
authors.lastname: Tulip
authors.forename: Robert
authors.initials: R
authors.lastname: Lindsay
mesh_terms: D000328:Adult; D000368:Aged; D002056:Burns / Q000503:physiopathology*; D017439:Cicatrix, Hypertrophic / Q000503:physiopathology*; D005260:Female; D006801:Humans; D017078:Laser-Doppler Flowmetry / Q000379:methods*; D008297:Male; D008875:Middle Aged; D012039:Regional Blood Flow; D012867:Skin / Q000098:blood supply*