title: Enhancing effects of mustard oil on preneoplastic hepatic foci development in Wistar rats.
abstract: Dietary habits are known to be the major contributory factor in the development of cancer. Mustard oil, which is extensively used in India and elsewhere as a flying and cooking medium, is reported to induce an inflammatory response. The development of altered hepatic foci is an early carcinogenic change in rat liver in diethylnitrosamine (DEN)-induced hepatocarcinogenesis. In the present study, the development of preneoplastic lesions was observed following administration of mustard oil (0.5 mL/day for 8 weeks) in DEN-initiated and partially hepatomized Wistar rats. A significant decrease in the relative and absolute liver weight of mustard oil-exposed rats was recorded. The results revealed a significant increase in the number and area of placental glutathione-S-transferase (GST-P) and gamma-glutamyl transpeptidase (GGT)-positive foci in mustard oil-administered animals. The GST-P- and GGT-positive foci were more prominent in the animals given boiled (up to 300 degrees C for 3 hours) mustard oil in comparison to the animals given fresh mustard oil. These results indicate the possible tumourigenic risk associated with mustard oil consumption.
authors.affiliation: Environmental Carcinogenesis Division, Industrial Toxicology Research Centre, MG Marg, PO Box No. 80, Lucknow 226 001, India. Yogeshwer_Shukla@hotmail.com
authors.forename: Yogeshwer
authors.initials: Y
authors.lastname: Shukla
authors.forename: Annu
authors.initials: A
authors.lastname: Arora
mesh_terms: D000818:Animals; D002273:Carcinogens / Q000633:toxicity; D004052:Diethylnitrosamine / Q000633:toxicity; D004357:Drug Synergism; D005982:Glutathione Transferase / Q000378:metabolism; D006498:Hepatectomy; D007124:Immunoenzyme Techniques; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced* / Q000201:enzymology / Q000473:pathology; D008297:Male; D009149:Mustard Plant; D009929:Organ Size / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D010938:Plant Oils; D011230:Precancerous Conditions / Q000139:chemically induced* / Q000201:enzymology / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D005723:gamma-Glutamyltransferase / Q000378:metabolism
chemical_list: D002273:Carcinogens; D010936:Plant Extracts; D010938:Plant Oils; D004052:Diethylnitrosamine; D005723:gamma-Glutamyltransferase; D005982:Glutathione Transferase; C027793:mustard oil