Susceptibility of ammonia-oxidizing bacteria to nitrification inhibitors.
Activity of nitrification inhibitors to several typical ammonia-oxidizing bacteria isolated recently, i. e. Nitrosococcus, Nitrosolobus, Nitrosomonas, Nitrosospira and Nitrosovibrio species was assayed using 2-amino-4-methyl-trichloromethyl-1,3,5-triazine (MAST), 2-amino-4-tribromomethyl-6-trichloromethyl-1,3,5-triazine (Br-MAST), 2-chloro-6-trichloromethylpyridine (nitrapyrin) and others, and compared to confirm the adequate control of ammonia-oxidizing bacteria by the inhibitors. The order of activity of the inhibitors to 13 species of ammonia-oxidizing bacteria examined was approximately summarized as Br-MAST > or = nitrapyrin > or = MAST > other inhibitors. Two Nitrosomonas strains, N. europaea ATCC25978 and N. sp. B2, were extremely susceptible to Br-MAST, exhibiting a pI50 > or = 6.40. These values are the position logarithms of the molar half-inhibition concentration. The 16S rRNA gene sequence similarity for the highly susceptible 4 strains of genus Nitrosomonas was 94% to 100% of Nitrosomonas europaea, although those of the less susceptible 3 strains of ammonia-oxidizing bacteria, Nitrosococcus oceanus C-107 ATCC19707, Nitrosolobus sp. PJA1 and Nitrosolobus multiformis ATCC25196, were 77.85, 91.53 and 90.29, respectively. However, no clear correlation has been found yet between pI50-values and percent similarity of 16S rRNA gene sequence among ammonia-oxidizing bacteria.
Matsuba
Douchi
D
Graduate School of Agricultural Science, Tamagawa University, Tamagawa-Gakuen, Machida-shi, Tokyo 194-8610, Japan.
Takazaki
Hirotoshi
H
Sato
Yukiharu
Y
Takahashi
Reiji
R
Tokuyama
Tatsuaki
T
Wakabayashi
Ko
K
D000641:Ammonia / Q000493:pharmacokinetics*; D041841:Bradyrhizobiaceae / Q000145:classification / Q000187:drug effects* / Q000378:metabolism*; D004275:DNA, Ribosomal / Q000235:genetics; D004777:Environment; D009569:Nitric Oxide / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism*; D009576:Nitrobacter / Q000187:drug effects / Q000378:metabolism; D009606:Nitrosomonas / Q000187:drug effects / Q000378:metabolism; D010084:Oxidation-Reduction; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014227:Triazines / Q000494:pharmacology*
D004275:DNA, Ribosomal; D009573:Nitrites; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D014227:Triazines; D009569:Nitric Oxide; D000641:Ammonia