A novel reiterated family of transcribed oligo(A)-terminated, interspersed DNA elements in the genome of Trypanosoma cruzi.
We report the molecular characterization of a novel reiterated family of transcribed oligo(A)-terminated, interspersed DNA elements in the genome of Trypanosoma cruzi. Steady-state level of transcripts of this sequence family appeared to be developmentally regulated, since only in the replicative forms the parasite showed expression of related sequences with a major band around 3 kb. The presence of frame shifts or premature stop codons predicts that transcripts are not translated. The sequence family also contains truncated forms of retrotransposons elements that may become potential hot spots for retroelement insertion. Sequences homologous to this family are interspersed at many chromosomes including the subtelomeric regions.
Verbisck
Newton Valério
NV
Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, São Paulo, SP, 04023-062, Brasil.
dos Santos
Márcia Regina Machado
MR
Engman
David M
DM
Angel Chiurillo
Miguel
M
Ramirez
José Luis
JL
Araya
Jorge Enrique
JE
Mortara
Renato Arruda
RA
da Silveira
José Franco
JF
D000818:Animals; D016054:DNA, Protozoan / Q000235:genetics*; D018503:Genome, Protozoan; D020071:Interspersed Repetitive Sequences / Q000235:genetics*; D014349:Trypanosoma cruzi / Q000235:genetics*
D016054:DNA, Protozoan