The effect of Calcicol as calcium tonic on delayed neurotoxicity induced by organophosphorus compounds.
To examine whether delayed neuropathy is prevented or alleviated when Ca is administered to experimental animals before or after organophosphorus compounds (OPs) dosing, we observed the effects of Calcicol administration as a calcium tonic on delayed neurotoxicity by OPs in hens. The hens (n=28) were randomly divided into seven groups (four in each group). One group received glycerol formal as vehicle group, two groups received 30 mg/kg leptophos or 40 mg/kg triortho-cresyl phosphate (TOCP) (L group and T group), two groups received 2.4 mg/kg Ca(2+) (0.3 ml/kg Calcicol) 24 h before leptophos or TOCP administration, and the last two groups received 2.4 mg/kg Ca after leptophos or TOCP administration, respectively. Although delayed polyneuropathy induced by OPs could not be prevented completely by Calcicol, the clinical signs of organophosphorus-induced delayed neuropathy (OPIDN) in hens that received Calcicol soon before or after OPs administration were less severe than those in hens that received only OPs and there were significant differences in OPIDN score between groups (P<0.05). This shows that polyneuropathy and the recovery function of nerves and muscles suffering from polyneuropathy can be alleviated, as long as calcium tonic is administered before the clinical signs develop. This study offers hope of recovery to humans who are exposed to these OPs because of work, attempted suicide, accidental ingestion or other accidents, etc. Meanwhile, our results indicate further that there is a relationship between a decrease in Ca(2+) concentration in tissues and induction of delayed neuropathy.
Piao
Fengyuan
F
Department of Public Health, School of Medicine, Mie University, 2-174 Edobashi, Tsu, Mie-Pref. 514-8507, Japan. piao-mie@doc.medic.mie-u.ac.jp
Yamauchi
Toru
T
Ma
Ning
N
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D002118:Calcium / Q000097:blood / Q000378:metabolism*; D002125:Calcium Gluconate / Q000494:pharmacology*; D002645:Chickens / Q000502:physiology*; D005260:Female; D020234:Gait Ataxia / Q000139:chemically induced; D007306:Insecticides / Q000037:antagonists & inhibitors / Q000633:toxicity; D010773:Leptophos / Q000037:antagonists & inhibitors / Q000633:toxicity; D020258:Neurotoxicity Syndromes / Q000517:prevention & control*; D009943:Organophosphorus Compounds / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D010243:Paralysis / Q000139:chemically induced; D014317:Tritolyl Phosphates / Q000037:antagonists & inhibitors / Q000633:toxicity
D007306:Insecticides; D009943:Organophosphorus Compounds; D014317:Tritolyl Phosphates; D010773:Leptophos; D002125:Calcium Gluconate; D002118:Calcium; C025541:tri-o-cresyl phosphate