Alternative splicing of cyclooxygenase-1 mRNA in the human iris.
In homogenates of the human iris, the nonsteroidal anti-inflammatory drug (NSAID) S(+)flurbiprofen has been reported to inhibit cyclooxygenase-1 (COX-1) 70-fold more potently than in human whole blood. We hypothesized that this difference may be due to alternative splicing of COX-1 mRNA in the human iris or in whole blood. In this study, we have identified a similar COX-1 splice variant (COX-1SV) in both tissues with comparable COX-1/COX-1SV expression ratios. Therefore, we conclude that the difference in IC(50) values of S(+)flurbiprofen towards COX-1 in the human iris and human whole blood is not related to differences in the occurrence of spliced COX-1.
Dröge
Melloney J
MJ
Department of Pharmaceutical Biology, University Centre for Pharmacy, Groningen, The Netherlands. m.j.droge@farm.rug.nl
van Sorge
Adriaan A
AA
van Haeringen
Nicolaas J
NJ
Quax
Wim J
WJ
Zaagsma
Johan
J
D000368:Aged; D000369:Aged, 80 and over; D017398:Alternative Splicing; D001769:Blood / Q000378:metabolism; D051545:Cyclooxygenase 1; D005260:Female; D006801:Humans; D007498:Iris / Q000378:metabolism*; D007527:Isoenzymes / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins; D008875:Middle Aged; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
D007527:Isoenzymes; D008565:Membrane Proteins; D012333:RNA, Messenger; D051545:Cyclooxygenase 1; C496511:PTGS1 protein, human; D011451:Prostaglandin-Endoperoxide Synthases