Interaction of chlorisondamine with the neuronal nicotinic acetylcholine receptor.
An epitope was found on the alpha2-nicotinic isoform of the neuronal nicotinic acetylcholine receptor that would likely form salt bridges with quaternary ammonium compounds and a cation-pi interaction with the pi-cloud of an aromatic ring. Chlorisondamine, a nicotinic antagonist, exerts a long-lasting, if not permanent, blockade of the ion channel gated by acetylcholine. Blocking of the ion channel prevents nicotine from exerting its rewarding effect on the CNS. Chlorisondamine contains two quaternary ammonium groups and a tetrachloroisoindoline ring. We propose that chlorisondamine interacts with an epitope on the alpha2 isoform of the rat neuronal nicotinic receptor (residues 388-402, GEREETEEEEEEEDE), where one or both of the quaternary ammonium groups of chlorisondamine form a salt bridge with dither a glutamic acid side chain or a phosphate group, whereas the tetrachlorobenzene portion of the tetrachloroisoindoline ring interacts with the guanidinium group of arginine in a cation-pi association: In this work, a new way of probing the interaction of a receptor epitope (alpha2) with organic molecules (chlorisondamine and hexachlorobenzene) was undertaken using matrix assisted laser desorption/ionization mass spectrometry.
Woods
Amina S
AS
The National Institute on Drug Abuse, Intramural Research Program, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland 21224, USA. awoods@intra.nida.nih.gov
Moyer
Susanne C
SC
Wang
Hay-Yan J
HY
Wise
Roy A
RA
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002714:Chlorisondamine / Q000378:metabolism*; D000939:Epitopes; D006581:Hexachlorobenzene / Q000378:metabolism; D013058:Mass Spectrometry; D009474:Neurons / Q000378:metabolism*; D010449:Peptide Mapping; D010766:Phosphorylation; D011485:Protein Binding; D020033:Protein Isoforms / Q000276:immunology / Q000378:metabolism; D051381:Rats; D011978:Receptors, Nicotinic / Q000276:immunology / Q000378:metabolism*
D000939:Epitopes; D020033:Protein Isoforms; D011978:Receptors, Nicotinic; D006581:Hexachlorobenzene; D002714:Chlorisondamine