Identification of a novel HLA-DRB1 allele: DRB1*1353.
In this report we describe a new HLA-DRB1 allele, DRB1*1353, which was initially recognized by a discrepancy between the results obtained with polymerase chain reaction using sequence-specific oligonucleotide (PCR-SSO) and sequence-specific primers (PCR-SSP). Sequence-based typing revealed sequence differences with other known HLA-DRB1 alleles. DRB1*1353 is identical to DRB1*13011 except for two nucleotide substitutions at nucleotide 84 (C(r)G) and at nucleotide 140 (T(r)A). These differences give rise to two amino acid substitutions at codons 28 and 47 from Asp to Glu and from Phe to Tyr, respectively.
Longhi
E
E
Centro Trasfusionale e di Immunologia dei Trapianti, Ospedale Maggiore Policlinico IRCCS, Milan, Italy.
Frison
S
S
Poli
F
F
Gaboardi
D
D
Ferrioli
G
G
Scalamogna
M
M
D000483:Alleles; D019943:Amino Acid Substitution; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D005260:Female; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006801:Humans; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid
D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D004247:DNA