title: Morphogenesis and proliferation in mono- and organotypic co-cultures of primary human periodontal ligament fibroblasts and alveolar bone cells.
abstract: Cells of the periodontal ligament and the alveolar bone lie in close vicinity in the periodontium. The goal of this study was to create an in vitro model to facilitate the study of the morphogenesis and proliferation of these two cell types under more in-vivo-like conditions. This was accomplished by the generation of organotypic co-cultures of primary human periodontal ligament fibroblasts (PDL) and alveolar bone cells (BC) and matched mono-cultures after 1, 2 and 3 weeks. Indirect immunofluorescence (IIF) for vimentin indicated that PDL cells exhibited sustained stratification only in the presence of BC cells, suggesting an important role for BCs in maintaining the stratification of PDL cells. In mono-cultures, only BC cells showed progressing stratification. They also displayed the most pronounced contraction of the cell culture matrix. Moreover, Ki-67 antigen detection by IIF revealed that these features coincided with cell proliferation localized on the matrix surface at the onset of cell stratification. These findings suggest that, in addition to proliferation, a further prerequisite for stratification may be cell migration. Furthermore, the maintained cell stratification, proliferation, and compartmentalization noted for PDL cells in organotypic co-cultures and BCs in mono-cultures can only be observed in a three-dimensional culture system. Thus, our system represents a novel experimental tool to further elucidate the underlying mechanisms of the growth and differentiation of PDL and bone tissue.
authors.affiliation: Department of Orthodontics and Dentofacial Orthopedics, Dental School, University of Heidelberg, Im Neuenheimer Feld 400, 69120 Heidelberg, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Reuther
authors.forename: A
authors.initials: A
authors.lastname: Kohl
authors.forename: G
authors.initials: G
authors.lastname: Komposch
authors.forename: P
authors.initials: P
authors.lastname: Tomakidi
mesh_terms: D001842:Bone and Bones / Q000166:cytology*; D002455:Cell Division; D002648:Child; D018920:Coculture Techniques; D024042:Collagen Type I / Q000378:metabolism; D003470:Culture Media; D005260:Female; D005347:Fibroblasts; D019084:Fluorescent Antibody Technique, Indirect; D005881:Gingiva / Q000166:cytology / Q000254:growth & development*; D006801:Humans; D019394:Ki-67 Antigen / Q000096:biosynthesis; D007700:Kinetics; D008022:Ligaments / Q000166:cytology / Q000254:growth & development*; D008297:Male; D014746:Vimentin / Q000378:metabolism
chemical_list: D024042:Collagen Type I; D003470:Culture Media; D019394:Ki-67 Antigen; D014746:Vimentin