Development of an adhesive sheet for direct counting of bacteria on solid surfaces.
An adhesive sheet was developed for direct counting of microorganisms on solid surfaces. The sheet consists of a polyurethane film base and water insoluble adhesive. SYBR Green II (for total direct counting) or 6-carboxyfluorescein diacetate (6CFDA) (for fluorescent vital staining) was used for fluorescent microscopy of bacteria collected on the adhesive face of the sheet. Adhesive sheet sampling showed a higher recovery rate for microbial enumeration than conventional swab method or stamp agar. This method is simple, rapid, inexpensive and reproducible.
Yamaguchi
Nobuyasu
N
Environmental Science and Microbiology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6, Yamada-oka, Suita, Japan.
Ishidoshiro
Ai
A
Yoshida
Yukari
Y
Saika
Takeshi
T
Senda
Shuji
S
Nasu
Masao
M
D000269:Adhesives / Q000737:chemistry*; D001431:Bacteriological Techniques / Q000379:methods; D015169:Colony Count, Microbial; D005456:Fluorescent Dyes; D008856:Microscopy, Fluorescence; D011140:Polyurethanes / Q000737:chemistry; D013499:Surface Properties
D000269:Adhesives; D005456:Fluorescent Dyes; D011140:Polyurethanes