Chimeric Newcastle disease virus nucleocapsid with parts of viral hemagglutinin-neuraminidase and fusion proteins.
The nucleocapsid (NP) protein of Newcastle disease virus (NDV) self-assembled in Escherichia coli as ring-like and herringbone-like particles. Several chimeric NP proteins were constructed in which the antigenic regions of the hemagglutinin-neuraminidase (HN) and fusion (F) proteins of NDV, myc epitope, and six histidines (a hexa-His tag) were linked to the C-terminus of the NP monomer. These chimeric proteins were expressed efficiently in soluble form in E. coli as detected by Western blot analysis. Electron microscopy of the purified products revealed that they self-assembled into ring-like particles. These chimeric particles exhibited antigenicity of the myc epitope, suggesting that the foreign sequences were exposed on the surface of the particles. Chickens inoculated with the chimeric particles mounted an immune response against NDV, suggesting the possibility of use of the ring-like particle as a carrier of immunogens in subunit vaccines and immunological reagents.
Rabu
A
A
Department of Biochemistry and Microbiology, Faculty of Science and Environmental Studies, University Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
Tan
W S
WS
Kho
C L
CL
Omar
A R
AR
Yusoff
K
K
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D002645:Chickens; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015707:HN Protein / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D009521:Newcastle Disease / Q000517:prevention & control* / Q000821:virology; D009522:Newcastle disease virus / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000378:metabolism; D014611:Vaccination; D014760:Viral Fusion Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology; D014771:Virion / Q000276:immunology; D019065:Virus Assembly
D000914:Antibodies, Viral; D015707:HN Protein; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D011993:Recombinant Fusion Proteins; D014760:Viral Fusion Proteins; D014764:Viral Proteins; D014765:Viral Vaccines; C409209:nucleoprotein, Newcastle disease virus