Study of the role of Chlamydia, Mycoplasma, Ureaplasma and other microaerophilic and aerobic bacteria in uterine infections of mares with reproductive disorders.
In six healthy mares and 24 mares showing reproductive disorders swab samples were taken from the fossa clitoridis to isolate Taylorella equigenitalis, and from the uterus to isolate mycoplasmas, ureaplasmas and other aerobic bacteria. Swab samples were also taken from the uterus for Chlamydia antigen ELISA and Chlamydia PCR studies. The uterus of 27 mares was examined cytologically, and biopsy samples were taken from the endometrium for histological examinations and for immunohistochemical examinations aimed at the detection of chlamydiae. T. equigenitalis, mycoplasmas, ureaplasmas and chlamydiae could not be detected from any of the mares examined. Aerobic facultative pathogenic bacteria were isolated from mares with endometritis in four cases. In 18 out of 22 mares with endometritis (82%) no infective agents could be demonstrated. Further studies are needed to elucidate the relative importance of non-infectious causes of endometritis and of anaerobic bacteria often detectable in the uterus in the aetiology of the reproductive disorders observed.
Szeredi
L
L
Central Veterinary Institute, H-1149 Budapest, Tábornok u. 2, Hungary. szeredil@oai.hu
Tenk
M
M
Schiller
I
I
Révész
T
T
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001420:Bacteria, Aerobic / Q000302:isolation & purification; D016022:Case-Control Studies; D002689:Chlamydia / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D004716:Endometritis / Q000382:microbiology / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D006734:Horse Diseases / Q000382:microbiology*; D006736:Horses / Q000382:microbiology; D007150:Immunohistochemistry; D009174:Mycoplasma / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000662:veterinary; D020625:Taylorella equigenitalis / Q000302:isolation & purification; D014509:Ureaplasma / Q000302:isolation & purification; D014599:Uterus / Q000166:cytology / Q000382:microbiology
D000942:Antigens, Bacterial; D004269:DNA, Bacterial