title: Type I collagen synthesis by human osteoblasts in response to placental lactogen and chaperonin 10, a homolog of early-pregnancy factor.
abstract: Recent studies have indicated that maternal skeletal metabolism undergoes significant changes during gestation. The agents that are responsible for eliciting these changes in bone turnover during pregnancy have yet to be defined. We therefore sought to investigate whether chaperonin 10 (Cpn10), a homolog of early-pregnancy factor, or human placental lactogen (PL) were capable of influencing the synthesis of type I collagen by human osteoblasts in vitro. Both Cpn10 and PL are major components of the maternal circulation during pregnancy, but how they might contribute to bone metabolism has not been determined. Type I collagen represents the most abundant component of bone tissue, accounting for approximately 90% of the organic compartment. Both Cpn10 and PL were capable of stimulating the synthesis of type I collagen by human osteoblasts in culture. The inclusion of 17 beta-estradiol or prolactin, however, failed to influence the ability of cells to mobilize type I collagen. These novel findings support a role for PL and Cpn10 in the metabolism of bone tissue during pregnancy. Maternal bone collagen metabolism is clearly an important event during pregnancy, and the identification of the factors responsible will aid our understanding of the regulation of skeletal metabolism during gestation.
authors.affiliation: Division of Child Dental Health, University of Bristol Dental School, Lower Maudlin Street, Bristol, United Kingdom BS1 2LY. j.p.mansell@bris.ac.uk
authors.forename: J P
authors.initials: JP
authors.lastname: Mansell
authors.forename: S J
authors.initials: SJ
authors.lastname: Yarram
authors.forename: N L
authors.initials: NL
authors.lastname: Brown
authors.forename: J R
authors.initials: JR
authors.lastname: Sandy
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D018835:Chaperonin 10 / Q000494:pharmacology*; D003094:Collagen / Q000096:biosynthesis*; D006801:Humans; D007700:Kinetics; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D010928:Placental Lactogen / Q000494:pharmacology*
chemical_list: D018835:Chaperonin 10; D003094:Collagen; D010928:Placental Lactogen; D020778:Matrix Metalloproteinase 2