title: Surface modification of poly(glycolic acid) (PGA) for biomedical applications.
abstract: The immobilization of biological ligands (such as biotin and peptides) onto biodegradable polymer surfaces, including poly(glycolic acid) (PGA) sutures, is complicated by the absence of functional groups on the polymer backbone. We demonstrate a method for overcoming this problem, by attaching (+)-biotinyl-3,6,9-trioxaundecanediamine to the surface of PGA sutures, which immobilizes the ligand through an amide bond between amine (ligands) and carboxylic acid groups (surface-hydrolyzed PGA sutures). Fluorescence microscopy was used to verify the attachment of the biotin ligand to the surface of the PGA suture after a complexation with fluorescein-conjugated streptavidin. The strategy can be generalized to surface modifications of other biodegradable aliphatic polyesters, which would improve the properties of the polymers in biomedical applications such as active targeting of drugs based on ligand-attached, polymeric drug delivery systems.
authors.affiliation: Department of Chemistry, School of Molecular Science-BK21, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea.
authors.forename: Kyung-Bok
authors.initials: KB
authors.lastname: Lee
authors.forename: Kuk Ro
authors.initials: KR
authors.lastname: Yoon
authors.forename: Seong Ihl
authors.initials: SI
authors.lastname: Woo
authors.forename: Insung S
authors.initials: IS
authors.lastname: Choi
mesh_terms: D001673:Biodegradation, Environmental; D001710:Biotin / Q000031:analogs & derivatives / Q000737:chemistry*; D016503:Drug Delivery Systems; D006868:Hydrolysis; D008024:Ligands; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D011100:Polyglycolic Acid / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry; D013499:Surface Properties; D013537:Sutures
chemical_list: D008024:Ligands; C480527:biotinyl-3,6,9-trioxaundecanediamine; D011100:Polyglycolic Acid; D001710:Biotin; D019809:Streptavidin