Ethylene rapidly up-regulates the activities of both monomeric GTP-binding proteins and protein kinase(s) in epicotyls of pea.
It is demonstrated that, in etiolated pea (Pisum sativum) epicotyls, ethylene affects the activation of both monomeric GTP-binding proteins (monomeric G-proteins) and protein kinases. For monomeric G-proteins, the effect may be a rapid (2 min) and bimodal up-regulation, a transiently unimodal activation, or a transient down-regulation. Pretreatment with 1-methylcyclopropene abolishes the response to ethylene overall. Immunoprecipitation studies indicate that some of the monomeric G-proteins affected may be of the Rab class. Protein kinase activity is rapidly up-regulated by ethylene, the effect is inhibited by 1-methylcyclopropene, and the activation is bimodal. Immunoprecipitation indicates that the kinase(s) are of the MAP kinase ERK1 group. It is proposed that the data support the hypothesis that a transduction chain exists that is separate and antagonistic to that currently revealed by studies on Arabidopsis mutants.
Moshkov
Igor E
IE
Timiryazev Institute of Plant Physiology RAS, Botanicheskaya 35, Moscow 127276, Russia.
Novikova
Galina V
GV
Mur
Luis A J
LA
Smith
Aileen R
AR
Hall
Michael A
MA
D003521:Cyclopropanes / Q000494:pharmacology; D005030:Ethylenes / Q000494:pharmacology*; D020559:Monomeric GTP-Binding Proteins / Q000378:metabolism*; D018532:Pisum sativum / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000378:metabolism; D011485:Protein Binding; D011494:Protein Kinases / Q000378:metabolism*; D015398:Signal Transduction; D015854:Up-Regulation / Q000187:drug effects*
D003521:Cyclopropanes; D005030:Ethylenes; D010937:Plant Growth Regulators; D010940:Plant Proteins; C105498:1-methylcyclopropane; C036216:ethylene; D011494:Protein Kinases; D020559:Monomeric GTP-Binding Proteins