Synchrony in human, mouse and bacterial cell cultures--a comparison.
Growth characteristics of synchronous human MOLT-4, human U-937 and mouse L1210 cultures produced with a new minimally-disturbing technology were compared to each other and to synchronous Escherichia coli B/r. Based on measurements of cell concentrations during synchronous growth, synchrony persisted in similar fashion for all cells. Cell size and DNA distributions in the mammalian cultures also progressed synchronously and reproducibly for multiple cell cycles. The results demonstrate that unambiguous multi-cycle synchrony, critical for verifying the absence of significant growth imbalances induced by the synchronization procedure, is feasible with these cell lines, and possibly others.
Helmstetter
Charles E
CE
Department of Biological Sciences, Florida Institute of Technology, Melbourne, FL 32901, USA.
Thornton
Maureen
M
Romero
Ana
A
Eward
K Leigh
KL
D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002455:Cell Division / Q000502:physiology*; D048429:Cell Size / Q000235:genetics; D004247:DNA / Q000378:metabolism; D004926:Escherichia coli / Q000166:cytology / Q000378:metabolism*; D005057:Eukaryotic Cells / Q000166:cytology / Q000378:metabolism*; D016193:G1 Phase / Q000235:genetics; D006801:Humans; D051379:Mice; D011387:Prokaryotic Cells / Q000166:cytology / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000378:metabolism*
D004247:DNA