title: High-performance ion-pair chromatography method for simultaneous analysis of alliin, deoxyalliin, allicin and dipeptide precursors in garlic products using multiple mass spectrometry and UV detection.
abstract: The quality of garlic and garlic products is usually related to their alliin content and allicin release potential. Until now no analytical method was able to quantify simultaneously allicin, its direct precursor alliin (S-allyl-L-cysteine sulfoxide), SAC (S-allyl-L-cysteine) as well as various dipeptides that apparently serve as storage compounds in garlic. It is well known that all these intermediates are involved in the allicin biosynthetic pathway. A simple and rapid HPLC method suitable for routine analysis was developed using eluents containing an ion-pairing reagent. Particularly, heptanesulfonate as ion-pairing reagent guarantees a sufficient separation between alliin and the more retained dipeptides at very low pH. Allicin was eluted after 18 min on a 150 x 3 mm column. Synthetic reference compounds were characterized by the same chromatographic method using a diode-array UV detector and an ion trap mass spectrometer (electrospray ionization) in the multiple MS mode. In routine analysis of garlic bulbs, powders and other products, the diode-array detector is sufficient for a relevant quantification. Our method has been used in studies to improve the quality of garlic and its derived products.
authors.affiliation: Université François Rabelais, IRBI, CNRS UMR 6035, Parc de Grandmont, 37200 Tours France.
authors.forename: I
authors.initials: I
authors.lastname: Arnault
authors.forename: J P
authors.initials: JP
authors.lastname: Christidès
authors.forename: N
authors.initials: N
authors.lastname: Mandon
authors.forename: T
authors.initials: T
authors.lastname: Haffner
authors.forename: R
authors.initials: R
authors.lastname: Kahane
authors.forename: J
authors.initials: J
authors.lastname: Auger
mesh_terms: D013437:Carbon-Sulfur Lyases / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003545:Cysteine / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D004151:Dipeptides / Q000032:analysis; D004220:Disulfides; D005737:Garlic / Q000737:chemistry*; D015394:Molecular Structure; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet; D013441:Sulfinic Acids / Q000032:analysis / Q000737:chemistry; D013455:Sulfur / Q000737:chemistry
chemical_list: D004151:Dipeptides; D004220:Disulfides; D013441:Sulfinic Acids; C006452:allicin; D013455:Sulfur; C006453:alliin; D013437:Carbon-Sulfur Lyases; C019243:alliin lyase; D003545:Cysteine