Electrochemically induced surface-enhanced infrared difference absorption (SEIDA) spectroscopy of a protein monolayer.
Functional infrared difference spectra of a protein monolayer are recorded by exploiting the surface enhancement exerted by a modified gold surface. The noble metal is used as the working electrode to induce redox-linked structural changes in the single layer of cytochrome c. The observed differences in the relative band intensities as compared to the difference spectrum of cytochrome c in solution are attributed to orientational effects of the adsorbed protein.
Ataka
Kenichi
K
Forschungszentrum Jülich, IBI-2: Structural Biology, 52425 Jülich, Germany.
Heberle
Joachim
J
D000818:Animals; D003574:Cytochrome c Group / Q000737:chemistry* / Q000378:metabolism; D004563:Electrochemistry / Q000379:methods*; D006736:Horses; D009206:Myocardium / Q000737:chemistry; D010084:Oxidation-Reduction; D013055:Spectrophotometry, Infrared / Q000379:methods*; D013499:Surface Properties
D003574:Cytochrome c Group