title: Induction of 5alpha-reductase type II mRNA transcription in primary cultured prostate epithelial cells by a soluble factor produced by primary cultured prostate fibroblast cells.
abstract: The role of DHT in the development of BPH has resulted in the formulation of several drugs, which have been designed to inhibit the formation of DHT by the 5alpha-reductase enzymes (5alpha-reductase type I (5alpha-RI) & 5alpha-reductase type II (5alpha-RII)). Although the function of these enzymes is well understood, the biochemical stimulus for initiation of 5alpha-RI and II gene expression has not been described. Study of a co-culture model indicated the presence of a diffusible factor secreted by prostatic fibroblast cells, which is responsible for the transcription of 5alpha-II mRNA in primary prostatic epithelial cells. In this study, we describe the partial characterisation of a fibroblast-secreted, soluble factor which we believe induces the transcription of 5alpha-RII mRNA in long-term primary cultures of prostate epithelial cells which can no longer transcribe 5alpha-RII mRNA.
authors.affiliation: Breast Cancer Research Group, Department of Oncology, University of Edinburgh, Paderewski Building, Western General Hospital, Crewe Road South, Edinburgh, EH4 2XU, UK. cbay@srv0.med.ed.ac.uk
authors.forename: C W
authors.initials: CW
authors.lastname: Bayne
authors.forename: M
authors.initials: M
authors.lastname: Ross
authors.forename: N F
authors.initials: NF
authors.lastname: Inglis
mesh_terms: D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase / Q000378:metabolism*; D002478:Cells, Cultured; D004790:Enzyme Induction; D004847:Epithelial Cells / Q000201:enzymology; D005347:Fibroblasts / Q000201:enzymology*; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D011467:Prostate / Q000201:enzymology*; D011470:Prostatic Hyperplasia / Q000201:enzymology; D012333:RNA, Messenger / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D014357:Trypsin / Q000494:pharmacology
chemical_list: D012333:RNA, Messenger; D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase; D014357:Trypsin