title: Effects of 50 Hz magnetic fields on fMLP-induced shape changes in invertebrate immunocytes: The role of calcium ion channels.
abstract: Plasma membrane Ca(2+) channels in immunocytes from the mussel Mytilus galloprovincialis exposed to 50 Hz sine wave magnetic fields (MFs) of various strengths were studied. At levels of 300 microT and above, MFs reduce shape changes in immunocytes induced by the chemotactic substance N-formyl-Meth-Leu-Phe, and this effect involves L-type Ca(2+) channels. Upon the addition of the Ca(2+) blocker verapamil to molluscan immunocytes exposed to MFs results in a synergistic cytotoxic action, while in the presence of the Ca(2+) opener SDZ-202, 791, a reactivation of the cells is observed. This suggests that, as previously reported for potassium channels, the damage to Ca(2+) channels induced by short exposure to MF at appropriate intensities is not permanent.
authors.affiliation: Departments of Hygiene, Microbiology, and Biostatistics, University of Modena and Reggio Emilia, Italy.
authors.forename: Fabriziomaria
authors.initials: F
authors.lastname: Gobba
authors.forename: Davide
authors.initials: D
authors.lastname: Malagoli
authors.forename: Enzo
authors.initials: E
authors.lastname: Ottaviani
mesh_terms: D000818:Animals; D049872:Bivalvia; D015220:Calcium Channels / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects*; D048429:Cell Size / Q000187:drug effects / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D004361:Drug Tolerance / Q000528:radiation effects; D004559:Electric Wiring; D004574:Electromagnetic Fields; D004781:Environmental Exposure / Q000032:analysis; D007448:Invertebrates; D007962:Leukocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D011829:Radiation Dosage; D014700:Verapamil / Q000494:pharmacology
chemical_list: D015220:Calcium Channels; D009240:N-Formylmethionine Leucyl-Phenylalanine; D014700:Verapamil