A modular on-line three-dimensional liquid chromatography-tandem mass spectrometry approach to characterization of organelle proteomes.
In this work we describe the use of a modular multidimensional chromatography-tandem mass spectrometry approach for rapid identification of proteins. In particular we highlight the use of a strong cation exchange cartridge in conjunction with a membrane postconcentration cartridge and nano-HPLC on-line with tandem mass spectrometry to characterize the eosinophil granule organelle proteome. Details are provided of the analytical approach we have developed and we discuss some of the advantages compared with previously reported analyses, as well as providing some specific examples of novel proteins identified.
Johnson
Kenneth L
KL
Biomedical Mass Spectrometry and Functional Proteomics Facility and Department of Biochemistry and Molecular Biology, Mayo Clinic/Foundation, Rochester, MN 55905, USA.
Loegering
David A
DA
Gleich
Gerald J
GJ
Naylor
Stephen
S
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004804:Eosinophils / Q000737:chemistry; D006801:Humans; D006868:Hydrolysis; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D015388:Organelles / Q000737:chemistry*; D020543:Proteome / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000737:chemistry
D020543:Proteome; D012710:Serum Albumin, Bovine