title: In vitro differentiation of human processed lipoaspirate cells into early neural progenitors.
abstract: Human processed lipoaspirate (PLA) cells are multipotent stem cells, capable of differentiating into multiple mesenchymal lineages (bone, cartilage, fat, and muscle). To date, differentiation to nonmesodermal fates has not been reported. This study demonstrates that PLA cells can be induced to differentiate into early neural progenitors, which are of an ectodermal origin. Undifferentiated cultures of human PLA cells expressed markers characteristic of neural cells such as neuron-specific enolase (NSE), vimentin, and neuron-specific nuclear protein (NeuN). After 2 weeks of treatment of PLA cells with isobutylmethylxanthine, indomethacin, and insulin, about 20 to 25 percent of the cells differentiated into cells with typical neural morphologic characteristics, accompanied by increased expression of NSE, vimentin, and the nerve-growth factor receptor trk-A. However, induced PLA cells did not express the mature neuronal marker, MAP, or the mature astrocyte marker, GFAP. It was also found that neurally induced PLA cells displayed a delayed-rectifier type K+ current (an early developmental ion channel) concomitantly with morphologic changes and increased expression of neural-specific markers. The authors concluded that human PLA cells might have the potential to differentiate in vitro into cells that represent early progenitors of neurons and/or glia.
authors.affiliation: Laboratory for Regenerative Bioengineering and Repair, Department of Surgery,University of California, Los Angeles, CALIF. 90095, USA.
authors.forename: Peter H
authors.initials: PH
authors.lastname: Ashjian
authors.forename: Amir S
authors.initials: AS
authors.lastname: Elbarbary
authors.forename: Brian
authors.initials: B
authors.lastname: Edmonds
authors.forename: Daniel
authors.initials: D
authors.lastname: DeUgarte
authors.forename: Min
authors.initials: M
authors.lastname: Zhu
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Zuk
authors.forename: H Peter
authors.initials: HP
authors.lastname: Lorenz
authors.forename: Prosper
authors.initials: P
authors.lastname: Benhaim
authors.forename: Marc H
authors.initials: MH
authors.lastname: Hedrick
mesh_terms: D000328:Adult; D000943:Antigens, Differentiation / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D015153:Blotting, Western; D002454:Cell Differentiation; D002469:Cell Separation; D002478:Cells, Cultured; D006801:Humans; D007150:Immunohistochemistry; D015187:Lipectomy; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000032:analysis; D009474:Neurons / Q000737:chemistry / Q000166:cytology* / Q000502:physiology; D018408:Patch-Clamp Techniques; D013234:Stem Cells / Q000737:chemistry / Q000166:cytology* / Q000502:physiology
chemical_list: D000943:Antigens, Differentiation; D000954:Antigens, Surface; D009419:Nerve Tissue Proteins