Cytokine release from osteoblasts in response to ultrasound stimulation.
Bone is a dynamic tissue with a well-balanced homeostasis preserved by both formation and resorption of bone. Normal turnover of bone, however, can be upset by either increased osteoclast activity or decreased osteoblast function; either mechanism alone or both may result in a net loss of bone. Both osteoclasts and osteoblasts could be stimulated by mechanical stimulation in vitro, and it is assumed that this process may occur in vivo as well. In this experiment, we investigated this hypothesis by examining the effects of ultrasound stimulation on osteoblast growth and cytokine release. With this model, we explored the mechanism of low-intensity pulsed ultrasound on osteoblasts growth and upregulation of osteoclasts formation and function by cytokine release. The results showed that specific pulsed ultrasound exposure could enhance osteoblasts population together with increase in TGFbeta1 secretion and decrease in concentration of IL-6 and TNFalpha in the culture medium. Although, animal studies and clinical trial are needed to understand the real process in the whole body, ultrasound stimulation might be a good method for prevention of bone loss due to osteoporosis.
Li
J K
JK
Department of Biomedical Engineering, Room 818, Building of Engineering, Chung Yuan Christian University, Chung-Li 32023, Taiwan.
Chang
W H
WH
Lin
J C
JC
Ruaan
R C
RC
Liu
H C
HC
Sun
J S
JS
D000818:Animals; D000831:Animals, Newborn; D002455:Cell Division / Q000502:physiology / Q000528:radiation effects; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis*; D004307:Dose-Response Relationship, Radiation; D005562:Formazans / Q000378:metabolism; D015850:Interleukin-6 / Q000096:biosynthesis; D040542:Mechanotransduction, Cellular / Q000502:physiology / Q000528:radiation effects; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism* / Q000502:physiology / Q000528:radiation effects*; D011829:Radiation Dosage; D051381:Rats; D017208:Rats, Wistar; D012886:Skull / Q000166:cytology / Q000378:metabolism / Q000502:physiology / Q000528:radiation effects; D013778:Tetrazolium Salts / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000096:biosynthesis; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D016207:Cytokines; D005562:Formazans; D015850:Interleukin-6; D013778:Tetrazolium Salts; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; C079782:MTT formazan