Binding of intimin from enteropathogenic Escherichia coli to lymphocytes and its functional consequences.
Intimin-conjugated fluorescent beads bind to spleen CD4 T cells and Peyer's patch, mesenteric lymph node, and cecal follicle lymphocytes, with less binding to lamina propria T cells and intraepithelial lymphocytes. Intimin costimulates proliferation of spleen CD4 T cells and cells from organized lymphoid tissues but does not costimulate cells from the lamina propria of normal or inflamed colon.
Gonçalves
Nathalie S
NS
School of Medicine, University of Southampton, Southampton General Hospital, Southampton SO16 6YD, United Kingdom.
Hale
Christine
C
Dougan
Gordon
G
Frankel
Gad
G
MacDonald
Thomas T
TT
D018829:Adhesins, Bacterial / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D017252:CD3 Complex / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism* / Q000494:pharmacology; D029968:Escherichia coli Proteins / Q000378:metabolism*; D005260:Female; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C
D018829:Adhesins, Bacterial; D017252:CD3 Complex; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; C073819:eaeA protein, E coli