title: Synthesis and biological evaluation of 14-alkoxymorphinans. 18. N-substituted 14-phenylpropyloxymorphinan-6-ones with unanticipated agonist properties: extending the scope of common structure-activity relationships.
abstract: The synthesis, biological, and pharmacological evaluations of 14beta-O-phenylpropyl-substituted morphinan-6-ones are described. The most striking finding of this study was that all of the compounds from the novel series of differently N-substituted 14beta-O-phenylpropylmorphinans acted as powerful opioid agonists. Even with N-substituents such as cyclopropylmethyl and allyl, which are usually associated with distinct antagonist properties, only agonists were obtained. Compared to morphine, the N-cyclopropylmethyl derivative 15 showed considerably increased potency in the in vivo assays in mice (600-fold in the tail-flick assay, 60-fold in the paraphenylquinone writhing test, and 400-fold in the hot-plate assay). Remarkably, most of the new ligands were nonselective and exhibited binding affinities in the subnanomolar range at opioid receptors (mu, kappa, delta), with the N-propyl derivative 19 displaying the highest affinity for the mu-receptor (K(i) = 0.09 nM).
authors.affiliation: Department of Pharmaceutical Chemistry, Institute of Pharmacy, University of Innsbruck, Innrain 52a, A-6020 Innsbruck, Austria.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Greiner
authors.forename: Mariana
authors.initials: M
authors.lastname: Spetea
authors.forename: Roland
authors.initials: R
authors.lastname: Krassnig
authors.forename: Falko
authors.initials: F
authors.lastname: Schüllner
authors.forename: Mario
authors.initials: M
authors.lastname: Aceto
authors.forename: Louis S
authors.initials: LS
authors.lastname: Harris
authors.forename: John R
authors.initials: JR
authors.lastname: Traynor
authors.forename: James H
authors.initials: JH
authors.lastname: Woods
authors.forename: Andrew
authors.initials: A
authors.lastname: Coop
authors.forename: Helmut
authors.initials: H
authors.lastname: Schmidhammer
mesh_terms: D000701:Analgesics, Opioid / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D008024:Ligands; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009019:Morphinans / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D010147:Pain Measurement; D011869:Radioligand Assay; D051381:Rats; D011957:Receptors, Opioid / Q000819:agonists*; D017465:Receptors, Opioid, delta / Q000819:agonists; D017473:Receptors, Opioid, kappa / Q000819:agonists; D017450:Receptors, Opioid, mu / Q000819:agonists; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured
chemical_list: D000701:Analgesics, Opioid; D008024:Ligands; D009019:Morphinans; D011957:Receptors, Opioid; D017465:Receptors, Opioid, delta; D017473:Receptors, Opioid, kappa; D017450:Receptors, Opioid, mu