[Alterations of p16 gene in transformed BEP2D cells induced by NNK].
Silver-staining PCR-SSCP method was used to detect the alterations of p16 gene in the transformed BEP2D cells induced by NNK. Compared with control cells, the abnormal shifting of the single-stranded DNA(ssDNA) in E1. beta but not p16 Exon 1-3 was identified in transformed BEP2D cells. The results suggested that the mutation and/or deletion in p16 gene might be one cause of NNK-unduced carcinogenesis in human bronchial epichellium.
Sun
H
H
Institute of Transfusion Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
Gong
Y
Y
Wang
Q
Q
Chen
J
J
D000818:Animals; D001980:Bronchi / Q000166:cytology*; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced / Q000235:genetics*; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology; D017353:Gene Deletion; D019942:Genes, p16; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009154:Mutation; D009602:Nitrosamines / Q000633:toxicity*
D009602:Nitrosamines; C016583:4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone