title: In vivo formation of salsolinol induced by high acetaldehyde concentration in rat striatum employing microdialysis.
abstract: OBJECTIVE
  The in vivo formation of salsolinol (1-methyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquionoline), an endogeneous condensation product of dopamine (DA) with acetaldehyde (AcH), was examined following the administration of cyanamide (CY) plus ethanol (EtOH) using microdialysis-high-performance liquid chromatography with electrochemical detection.
  
  
  METHODS
  After the insertion of a microdialysis probe into the striatum, rats were treated with CY (a potent inhibitor of aldehyde dehydrogenase, 50 mg/kg), 4-methylpyrazole (4-MP, a strong inhibitor of alcohol dehydrogenase, 82 mg/kg), and CY + 4-MP, followed 1 h later by EtOH (1 g/kg), CY and 4-MP only by intraperitoneal administration.
  
  
  RESULTS
  In the CY + EtOH group, salsolinol was detected in striatal dialysates and high AcH concentrations were found in the blood. The time course of changes in salsolinol concentrations correlated with blood AcH concentrations. In the other experimental groups, salsolinol in the dialysates and high AcH concentrations in the blood were not detected.
  
  
  CONCLUSIONS
  These observations indicate that: (1) high AcH concentrations induce the formation of salsolinol in the rat striatum; (2) there is no effect of EtOH or AcH on striatal dialysate concentrations of DA and 5-hydroxytryptamine.
authors.affiliation: Department of Forensic Medicine, Faculty of Medicine, Kagawa Medical University, 1750-1, Ikenobe, Miki, Kita, Kagawa 761-0793, Japan.
authors.forename: Mostofa
authors.initials: M
authors.lastname: Jamal
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Ameno
authors.forename: Takako
authors.initials: T
authors.lastname: Kubota
authors.forename: Setsuko
authors.initials: S
authors.lastname: Ameno
authors.forename: Xia
authors.initials: X
authors.lastname: Zhang
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Kumihashi
authors.forename: Iwao
authors.initials: I
authors.lastname: Ijiri
mesh_terms: D000079:Acetaldehyde / Q000097:blood / Q000494:pharmacology*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism; D003484:Cyanamide / Q000008:administration & dosage; D004298:Dopamine / Q000032:analysis / Q000378:metabolism*; D000431:Ethanol / Q000097:blood / Q000494:pharmacology*; D007546:Isoquinolines / Q000032:analysis / Q000378:metabolism*; D008297:Male; D017551:Microdialysis / Q000379:methods; D051381:Rats; D017208:Rats, Wistar
chemical_list: D007546:Isoquinolines; D000431:Ethanol; D003484:Cyanamide; C036617:salsolinol; D000079:Acetaldehyde; D004298:Dopamine