[Characterization of lipopolysaccharides from Ralstonia solanacearum].
Lipopolysaccharides (LPSs) from four strains of Ralstonia solanacearum belonging to biovar I (ICMP 6524, 8115, 5712, and 8169) were isolated and investigated. The structural components of the LPS molecule, such as lipid A, the core oligosaccharide, and O-specific polysaccharide (O-PS), were obtained after mild acid hydrolysis of the LPS preparations. In lipid A from all the LPS samples studied, 3-hydroxyhexadecanoic, 2-hydroxyhexadecanoic, tetradecanoic, and hexadecanoic fatty acids prevailed. The dominant monosaccharides of the core oligosaccharides of all of the strains studied were rhamnose, glucose, glucosamine, 2-keto-3-deoxyoctulosonic acid, and heptose. However, individual strains varied in the content of galactose, ribose, xylose, and arabinose. Three types of the O-PS structure were established, which differed in their configuration (alpha or beta), as well as in the type of the bond between glucosamine and rhamnose residues (1-->2 or 1-->3).
Varbanets
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LD
Zabolotnyi Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, ul. Zabolotnogo 154, Kiev, 252143 Ukraine.
Vasil'ev
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VN
Brovarskaia
O S
OS
D002849:Chromatography, Gas; D005944:Glucosamine / Q000032:analysis; D005947:Glucose / Q000032:analysis; D016946:Gram-Negative Aerobic Rods and Cocci / Q000737:chemistry*; D006539:Heptoses / Q000032:analysis; D008050:Lipid A / Q000737:chemistry; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D019814:Myristic Acid / Q000032:analysis; D019081:O Antigens / Q000737:chemistry; D019308:Palmitic Acid / Q000032:analysis; D010169:Palmitic Acids / Q000032:analysis; D012210:Rhamnose / Q000032:analysis; D013400:Sugar Acids / Q000032:analysis
D006539:Heptoses; D008050:Lipid A; D008070:Lipopolysaccharides; D019081:O Antigens; D010169:Palmitic Acids; D013400:Sugar Acids; D019814:Myristic Acid; C002532:2-keto-3-deoxyoctonate; D019308:Palmitic Acid; C024707:2-hydroxyhexadecanoic acid; D005947:Glucose; D005944:Glucosamine; D012210:Rhamnose
Kharakteristika lipopolisakharidov Ralstonia solanacearum.