title: Deficiency of different nitric oxide synthase isoforms activates divergent transcriptional programs in cardiac hypertrophy.
abstract: Decreased nitric oxide synthase (NOS) activity induces left ventricular hypertrophy (LVH), but the transcriptional pathways mediating this effect are unknown. We hypothesized that specific NOS isoform deletion (NOS3 or NOS1) would activate different transcriptional programs in LVH. We analyzed cardiac expression profiles (Affymetrix MG-U74A) from NOS-/- mice using robust multi-array average (RMA). Of 12,422 genes analyzed, 47 genes were differentially expressed in NOS3-/- and 67 in NOS1(-/-) hearts compared with wild type (WT). Only 16 showed similar changes in both NOS-/- strains, most notably decreased heat-shock proteins (HSP10, 40, 70, 86, 105). Hypertrophied NOS1-/- hearts had unique features, including decreased myocyte-enriched calcineurin interacting protein and paradoxical downregulation of fetal isoforms (alpha-skeletal actin and brain natriuretic peptide). Cluster analyses demonstrated that NOS1 deletion caused more pronounced changes in the myocardial transcriptome than did NOS3 deletion, despite similar cardiac phenotypes. These findings suggest that the transcriptional basis for LVH varies depending on the inciting biochemical stimulus. In addition, NOS isoforms appear to play distinct roles in modulating cardiac structure.
authors.affiliation: National Heart, Lung, and Blood Institute Program in Genomic Applications-HopGene, Philadelphia, Pennsylvania 19104, USA.
authors.forename: Thomas P
authors.initials: TP
authors.lastname: Cappola
authors.forename: Leslie
authors.initials: L
authors.lastname: Cope
authors.forename: Amy
authors.initials: A
authors.lastname: Cernetich
authors.forename: Lili A
authors.initials: LA
authors.lastname: Barouch
authors.forename: Khalid
authors.initials: K
authors.lastname: Minhas
authors.forename: Rafael A
authors.initials: RA
authors.lastname: Irizarry
authors.forename: Giovanni
authors.initials: G
authors.lastname: Parmigiani
authors.forename: Sarfraz
authors.initials: S
authors.lastname: Durrani
authors.forename: Tera
authors.initials: T
authors.lastname: Lavoie
authors.forename: Eric P
authors.initials: EP
authors.lastname: Hoffman
authors.forename: Shui Q
authors.initials: SQ
authors.lastname: Ye
authors.forename: Joe G N
authors.initials: JG
authors.lastname: Garcia
authors.forename: Joshua M
authors.initials: JM
authors.lastname: Hare
mesh_terms: D000818:Animals; D006332:Cardiomegaly / Q000201:enzymology* / Q000235:genetics*; D016000:Cluster Analysis; D020869:Gene Expression Profiling / Q000706:statistics & numerical data; D005786:Gene Expression Regulation / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D007527:Isoenzymes / Q000172:deficiency / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D019001:Nitric Oxide Synthase / Q000172:deficiency* / Q000235:genetics*; D052248:Nitric Oxide Synthase Type I; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; D016133:Polymerase Chain Reaction / Q000379:methods / Q000706:statistics & numerical data; D014158:Transcription, Genetic / Q000235:genetics* / Q000502:physiology
chemical_list: D007527:Isoenzymes; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; C496321:Nos1 protein, mouse; C496315:Nos3 protein, mouse