Cyclin D1 genotype in areca-associated oral squamous cell carcinoma.
BACKGROUND
Oral squamous cell carcinoma (OSCC) is the most common malignancy in areca-chewing regions, accounting for up to 50% of malignant tumors in some South Asian countries. Amplification and/or over-expression of cyclin D1 (CCND1) is a frequent event in human malignancies, including OSCC. CCND1 G870A polymorphism (codon 242) gives rise to two isoforms of the protein. The objective of the present study was to evaluate if the risk, onset, and prognosis of areca-associated OSCC is related to CCND1 genotypes.


METHODS
We analyzed the CCND1 genotype in 70 OSCC cases and 93 control Taiwanese using single-strand conformation polymorphism techniques.


RESULTS
Statistical analysis showed that CCND1 genotype had no impact on the risk, onset, or survival of areca-associated OSCC. However, buccal squamous cell carcinoma (BSCC) appeared to be less frequently associated with AA genotype than non-BSCC (P = 0.02). In addition, amplification of CCND1 was significantly more prevalent in OSCC cases (22%) than in control subjects (2%, P < 0.01).


CONCLUSIONS
This study demonstrates that the CCND1 genotype may confer different risks for BSCC and non-BSCC.
Wong
Yong-Kie
YK
School of Dentistry, National Yang Ming University, Taipei, Taiwan.
Lin
Shu-Chun
SC
Chang
Che-Shoa
CS
Tseng
Yu-Hsin
YH
Liu
Chung-Ji
CJ
Lin
Huey-Ching
HC
Chang
Kuo-Wei
KW
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001114:Areca / Q000633:toxicity; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced / Q000453:epidemiology / Q000235:genetics*; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D015897:Comorbidity; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D019940:Genes, bcl-1 / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000139:chemically induced / Q000453:epidemiology / Q000235:genetics*; D018570:Risk Assessment; D012907:Smoking / Q000453:epidemiology; D013624:Taiwan