title: Nucleotide sequence of a Thai isolate of Papaya ringspot virus type W.
abstract: The complete nucleotide sequence of a Thai isolate of Papaya ringspot virus (PRSV) type W (PRSV-W) was determined. The viral genome is 10,323 nucleotides (nts) long and contains an ORF encoding polyprotein 3,343 amino acids (aa) long, flanked with 5'- and 3'-non-coding regions (NCRs) of 85 and 206 nts, respectively. Out of the ten putative proteins P1 is the most variable (73.9% similarity) as compared to the PRSV type P (PRSV-P) sequences, while the CI protein is most conserved (99.1% similarity). The sequence similarity among the type W and P isolates also suggests that the P type arose from the W type. However, no significant difference between types P and W that would account for the host specificity was disclosed.
authors.affiliation: Institute of Molecular Biology and Genetics, Mahidol University, Salaya Campus, Nakhon Pathom 73170, Thailand.
authors.forename: P
authors.initials: P
authors.lastname: Attasart
authors.forename: G
authors.initials: G
authors.lastname: Charoensilp
authors.forename: S
authors.initials: S
authors.lastname: Kertbundit
authors.forename: S
authors.initials: S
authors.lastname: Panyim
authors.forename: M
authors.initials: M
authors.lastname: Juricek
mesh_terms: D001483:Base Sequence; D029441:Carica / Q000821:virology*; D018076:DNA, Complementary; D016679:Genome, Viral; D008969:Molecular Sequence Data; D017800:Potyvirus / Q000235:genetics* / Q000302:isolation & purification*; D012367:RNA, Viral / Q000235:genetics; D013785:Thailand
chemical_list: D018076:DNA, Complementary; D012367:RNA, Viral