Cytotoxic effects of two antimolting insecticides in mammalian CHO-K1 cells.
Cytotoxicity of two insect growth regulators, diflubenzuron, a benzoylphenylurea derivative that inhibits the synthesis of new chitin in target organisms, and pyriproxyfen, an insect juvenile hormone analogue, were tested on CHO-K1 cultures, using the neutral red incorporation assay. Both compounds displayed cytotoxic effects that rise with time exposure. The presence of either fetal calf serum or bovine serum albumin diminished significantly the cytotoxicity of both compounds, thus pointing to a strong protein binding. In addition, extensive metabolization with rat liver submitochondrial fraction gave rise to metabolites less toxic than the parent compounds, implying the relative safety of both diflubenzuron and pyriproxyfen in mammals.
Bayoumi
A E
AE
Department of Plant Protection, Faculty of Agriculture, University Ain Shams, Cairo, Egypt.
Pérez-Pertejo
Y
Y
Zidan
H Z
HZ
Balaña-Fouce
R
R
Ordóñez
C
C
Ordóñez
D
D
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D004132:Diflubenzuron / Q000378:metabolism / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D007605:Juvenile Hormones; D008099:Liver; D008928:Mitochondria; D011485:Protein Binding; D011725:Pyridines / Q000378:metabolism / Q000633:toxicity*; D051381:Rats
D007605:Juvenile Hormones; D011725:Pyridines; C055613:pyriproxyfen; D004132:Diflubenzuron