title: Physiological characteristics of the biocontrol yeast Pichia anomala J121.
abstract: The yeast Pichia anomala J121 prevents mold spoilage and enhances preservation of moist grain in malfunctioning storage systems. Development of P. anomala J121 as a biocontrol agent requires in-depth knowledge about its physiology. P. anomala J121 grew under strictly anaerobic conditions, at temperatures between 3 degrees C and 37 degrees C, at pH values between 2.0 and 12.4, and at a water activity of 0.92 (NaCl) and 0.85 (glycerol). It could assimilate a wide range of C- and N-sources and produce killer toxin. A selective medium containing starch, nitrate, acetic acid, and chloramphenicol was developed for P. anomala. P. anomala was equally sensitive as Candida albicans to common antifungal compounds. Growth ability at a range of environmental conditions contributes to the competitive ability of the biocontrol yeast P. anomala J121.
authors.affiliation: Department of Microbiology, Swedish University of Agricultural Sciences (SLU), P.O. Box 7025, SE-750 07 Uppsala, Sweden. elisabeth.fredlund@mikrob.slu.se
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Fredlund
authors.forename: Ulrika
authors.initials: U
authors.lastname: Druvefors
authors.forename: Marianne E
authors.initials: ME
authors.lastname: Boysen
authors.forename: Karl-Johan
authors.initials: KJ
authors.lastname: Lingsten
authors.forename: Johan
authors.initials: J
authors.lastname: Schnürer
mesh_terms: D000693:Anaerobiosis; D000898:Antibiosis; D003470:Culture Media; D005658:Fungi / Q000254:growth & development*; D010572:Pest Control, Biological; D010843:Pichia / Q000502:physiology*
chemical_list: D003470:Culture Media