Role of apoptosis in the remodeling of cholestatic liver injury following release of the mechanical stress.
It has been known for a long time that portal fibrosis consecutive to experimental common bile duct ligation is reversible following obstacle removal, but the mechanisms involved remain unknown. We have studied the effect of bilioduodenal anastomosis and of simple biliary decompression on the remodeling of the lesion in bile duct-ligated rats. Rats were subjected to common bile duct ligation for 7 days or 14 days. Bilioduodenal anastomosis was performed after 14 days of bile duct ligation and animals sacrificed at intervals. In other animals, after 7 days or 14 days of ligation, the common bile duct was merely decompressed by bile aspiration and animals sacrificed 24 h later. Collagen deposition, alpha-smooth muscle actin expression and apoptosis were evaluated. Bile was collected and the bile acid profile assessed. After anastomosis, collagen deposition and alpha-smooth muscle actin expression decreased and were back to control values after 7 days. These parameters remained practically unchanged 24 h after biliary decompression. Bile duct ligation by itself induced apoptosis of some fibroblastic and bile ductular cells after 7 days; this was back to normal after 14 days. After anastomosis or decompression, apoptosis of both fibroblastic and bile ductular cells increased greatly and was accompanied by ultrastructural features of extracellular matrix degradation. Total bile acid content decreased after common bile duct ligation, the proportion of dihydroxylated bile acids decreasing and that of trihydroxylated bile acids increasing. Biliary decompression and anastomosis did not modify total concentration and composition of the biliary bile acid pool. In summary, we show that mere biliary decompression, by relieving the mechanical stress, is as effective as bilioduodenal anastomosis to induce apoptosis of portal cells that likely triggers portal fibrosis regression.
Costa
Andréa M A
AM
Groupe de Recherches pour l'Etude du Foie, INSERM E0362, Université Victor Segalen Bordeaux 2, 146 rue Léo-Saignat, France.
Tuchweber
Beatriz
B
Lamireau
Thierry
T
Yousef
Ibrahim M
IM
Balabaud
Charles
C
Rosenbaum
Jean
J
Desmoulière
Alexis
A
D000199:Actins / Q000378:metabolism; D000714:Anastomosis, Surgical; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001646:Bile / Q000737:chemistry; D001647:Bile Acids and Salts / Q000032:analysis; D002780:Cholestasis, Intrahepatic / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D003094:Collagen / Q000378:metabolism; D003135:Common Bile Duct / Q000601:surgery; D019299:Decompression, Surgical; D004195:Disease Models, Animal; D004386:Duodenum / Q000601:surgery; D008026:Ligation; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008105:Liver Cirrhosis, Biliary / Q000150:complications / Q000378:metabolism / Q000473:pathology*; D008106:Liver Cirrhosis, Experimental / Q000150:complications / Q000378:metabolism / Q000473:pathology*; D008297:Male; D011168:Portal System / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D013314:Stress, Mechanical
D000199:Actins; D001647:Bile Acids and Salts; D003094:Collagen