The endonuclease NL1Tc encoded by the LINE L1Tc from Trypanosoma cruzi protects parasites from daunorubicin DNA damage.
In the present paper we show that the overexpression of the NL1Tc protein, encoded by the L1Tc non-LTR retrotransposon from Trypanosoma cruzi, led to a reduction of about 60% of DNA damage caused by daunorubicin treatment. This repair effect is not observed in transfected parasites overexpressing the NL1Tc mutated in the aspartic acid located in the active site of the enzyme. In addition, NL1Tc overexpression protects the parasite from the negative effect that daunorubicin has on parasite's growth rate. Thus, parasites overexpressing NL1Tc show, after treatment with 4 microM of daunorubicin, growth rate two to three times higher than the growth rate observed in treated control parasites transformed with the empty vector or overexpressing the mutated NL1Tc. Likewise, parasites overexpressing the NL1Tc protein and irradiated with a single dose of gamma-radiation (6 or 9 Gy) show higher growth rates than the parasites overexpressing the mutated NL1Tc or the control transfected parasites.
Olivares
M
M
Departamento de Biología Molecular, Instituto de Parasitología y Biomedicina López Neyra, CSIC, 18001, Granada, Spain.
López
M C
MC
García-Pérez
J L
JL
Briones
P
P
Pulgar
M
M
Thomas
M C
MC
D000818:Animals; D004249:DNA Damage; D004260:DNA Repair; D003630:Daunorubicin / Q000633:toxicity; D004720:Endonucleases / Q000235:genetics / Q000502:physiology*; D005720:Gamma Rays; D018626:Retroelements; D014170:Transformation, Genetic; D014349:Trypanosoma cruzi / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000528:radiation effects
D018626:Retroelements; D004720:Endonucleases; D003630:Daunorubicin