title: Association between the Pl(A) platelet glycoprotein GPIIIa polymorphism and extent of coronary artery disease.
abstract: BACKGROUND
  The Pl(A2) allele of the gene encoding for GPIIIa subunit of the platelet membrane receptor glycoprotein (GP) IIb/IIIa has been suggested as a significant risk factor for thrombotic complications of coronary artery disease (CAD). The aim of the current investigation was to investigate the association between Pl(A) GPIIIa polymorphism and the extent of angiographically confirmed CAD in patients from the north region of Poland.
  
  
  METHODS
  The study was performed in 397 male Caucasian patients. All subjects had significant coronary artery stenosis confirmed by elective coronary angiography. Screening for the Pl(A) GPIIIa genotypes was performed by polymerase chain reaction of genomic DNA, followed by NciI digestion and agarose gel electrophoresis.
  
  
  RESULTS
  The genotype distribution of the Pl(A) GPIIIa polymorphism in our study group was Pl(A1/A1)-75%, Pl(A1/A2)-24% and Pl(A2/A2)-1% with Pl(A1) and Pl(A2) allele frequencies of 0.87 and 0.13, respectively. The prevalence of the homozygous Pl(A1/A1) genotype among subjects with multiple-vessel CAD (two or three vessels with at least 50% stenosis) was significantly higher than in patients with single-vessel disease; the odds ratio of Pl(A2/A2) or Pl(A1/A2) patients for having multiple-vessel CAD was 0.46 (95% CI 0.27-0.77, P<0.01). The mean CAD score for Pl(A1/A1) patients was significantly higher in comparison to Pl(A2/A2) and Pl(A1/A2) patients (7.58+/-2.20 and 6.98+/-2.37, respectively, P<0.05).
  
  
  CONCLUSIONS
  Our results suggest, that the Pl(A1/A1) genotype of Pl(A) GPIIIa polymorphism is associated with more severe CAD in male Caucasian patients from the north region of Poland.
authors.affiliation: First Department of Cardiology, Medical University of Gdansk, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Gruchała
authors.forename: Dariusz
authors.initials: D
authors.lastname: Ciećwierz
authors.forename: Karolina
authors.initials: K
authors.lastname: Ochman
authors.forename: Radosław
authors.initials: R
authors.lastname: Targoński
authors.forename: Witold
authors.initials: W
authors.lastname: Dubaniewicz
authors.forename: Wojciech
authors.initials: W
authors.lastname: Sobiczewski
authors.forename: Bartosz
authors.initials: B
authors.lastname: Wasag
authors.forename: Piotr
authors.initials: P
authors.lastname: Drewla
authors.forename: Pawel
authors.initials: P
authors.lastname: Skarzynski
authors.forename: Piotr
authors.initials: P
authors.lastname: Romanowski
authors.forename: Janusz
authors.initials: J
authors.lastname: Limon
authors.forename: Andrzej
authors.initials: A
authors.lastname: Rynkiewicz
mesh_terms: D000328:Adult; D000368:Aged; D017023:Coronary Angiography; D003324:Coronary Artery Disease / Q000000981:diagnostic imaging / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics*; D008875:Middle Aged; D010960:Plasminogen Activators / Q000235:genetics*; D011044:Poland; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D012720:Severity of Illness Index; D044465:White People / Q000235:genetics*
chemical_list: D008562:Membrane Glycoproteins; C040580:glycoprotein GP III; D010960:Plasminogen Activators