title: Antimicrobial activity of endodontic sealers on Enterococcus faecalis.
abstract: Enterococcus faecalis has been shown to be highly resistant once established in the root canal system and may play an important role in endodontic failures. The purpose of this study was to evaluate the antimicrobial activity of four root canal sealers on E. faecalis. Seventeen blood-agar plates were inoculated with E. faecalis using the Lawn technique. Five discs were placed on each plate, four with sealers--Sealapex, Roth 811, Kerr EWT, and AH-Plus--and an ampicillin disc as the control. The plates were incubated at 37 degrees C. The zones of inhibition were measured at 24 and 48 h. Analysis using a one-way ANOVA and Tukey test showed a statistically significance difference (p < 0.05) between all four groups of sealers. Roth 811 showed the largest zone of inhibition (1.1 mm), followed by Sealapex (0.8 mm) and Kerr EWT (0.5 mm), whereas AH-Plus had no antimicrobial activity. There was no difference in the zones of inhibition between the 24- and 48-h time periods.
authors.affiliation: Department of Endodontics, Case Western Reserve University, School of Dentistry, Cleveland, OH 44106-4905, USA.
authors.forename: André K
authors.initials: AK
authors.lastname: Mickel
authors.forename: Tuan H
authors.initials: TH
authors.lastname: Nguyen
authors.forename: Sami
authors.initials: S
authors.lastname: Chogle
mesh_terms: D000704:Analysis of Variance; D000891:Anti-Infective Agents, Local / Q000494:pharmacology; D002126:Calcium Hydroxide / Q000494:pharmacology; D013293:Enterococcus faecalis / Q000187:drug effects*; D004853:Epoxy Resins / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D012459:Salicylates / Q000494:pharmacology; D018709:Statistics, Nonparametric; D015035:Zinc Oxide-Eugenol Cement / Q000494:pharmacology
chemical_list: D000891:Anti-Infective Agents, Local; D004853:Epoxy Resins; D012387:Root Canal Filling Materials; D012459:Salicylates; D015035:Zinc Oxide-Eugenol Cement; C534916:epoxy resin-based root canal sealer; C055601:Sealapex; C095719:Roth's 811 sealer; C076543:Kerr Pulp Canal Sealer; D002126:Calcium Hydroxide