Expression of ETS-1 related to angiogenesis in uterine endometrium during the menstrual cycle.
ETS-1 has been identified as a transcription factor for angiogenesis, which is essential for the development and growth of the uterine endometrium. This characteristic prompted us to study whether ETS-1 functions as an angiogenic mediator in uterine endometrium. Immunohistochemical staining revealed that the localization of ETS-1 was similar to that of vascular endothelial cells. There was a significant correlation between microvessel counts and both ETS-1 histoscores and ets-1 mRNA levels in uterine endometrium. The ETS-1 histoscores and ets-1 mRNA levels increased in the proliferative phase, reached a peak during peri-ovulation and decreased in the secretory phase. Furthermore, the ETS-1 histoscores and ets-1 mRNA levels correlated with vascular endothelial growth factor (VEGF) levels in the proliferative phase. This indicates that ETS-1 might be an angiogenic mediator in uterine endometrium linked to VEGF in the proliferative phase.
Fujimoto
Jiro
J
Department of Obstetrics and Gynecology, Gifu University School of Medicine, 40 Tsukasa-machi, Gifu City 500-8705, Japan. jf@cc.gifu-u.ac.jp
Aoki
Ikumi
I
Toyoki
Hiroshi
H
Khatun
Sufia
S
Sato
Eriko
E
Tamaya
Teruhiko
T
D000328:Adult; D043925:Angiogenesis Inducing Agents / Q000378:metabolism*; D015139:Blotting, Southern; D017931:DNA Primers; D004717:Endometrium / Q000378:metabolism / Q000502:physiology*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008597:Menstrual Cycle; D008875:Middle Aged; D018919:Neovascularization, Physiologic / Q000502:physiology*; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins / Q000378:metabolism / Q000502:physiology*; D050783:Proto-Oncogene Proteins c-ets; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000378:metabolism / Q000502:physiology*
D043925:Angiogenesis Inducing Agents; D017931:DNA Primers; C494041:ETS1 protein, human; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D014157:Transcription Factors