HLA associations with occupational sensitization to rat lipocalin allergens: a model for other animal allergies?
BACKGROUND
Laboratory animal allergy is a common occupational health problem affecting between 11% and 44% of exposed researchers. Allergy to rats and mice is most common, probably because these are the animals most frequently used.


OBJECTIVE
We hypothesized that HLA class II molecules, involved in the presentation of allergen to the T cell and likely candidates for controlling the immune response, might be associated with sensitization to rat urinary proteins among laboratory animal handlers.


METHODS
We undertook a cross-sectional study of 741 employees at 6 pharmaceutical sites across the United Kingdom who had contact at work with laboratory rats. In all, 109 cases with specific sensitization to rat proteins and 397 referents were HLA-typed for DRB1 and DQB1 loci. Amino acid analyses of significantly associated HLA molecules were carried out.


RESULTS
HLA-DR7 was associated with sensitization (odds ratio [OR], 1.82; CI, 1.12-2.97), respiratory symptoms at work (OR, 2.96; CI, 1.64-5.37) and, most strongly, sensitization with symptoms (OR, 3.81; CI, 1.90-7.65). HLA-DR3 was protective against sensitization (OR, 0.55; CI, 0.31-0.97). Amino acid analyses of these 2 molecules indicated a biologically plausible explanation for the associations.


CONCLUSIONS
HLA phenotype is an important determinant of individual susceptibility to sensitization and asthma among laboratory animal workers. Similar mechanisms might apply in other animal allergies.
Jeal
Hayley
H
Department of Occupational and Environmental Medicine, Imperial College (NHLI), London, United Kingdom.
Draper
Adrian
A
Jones
Meinir
M
Harris
Jessica
J
Welsh
Ken
K
Taylor
Anthony Newman
AN
Cullinan
Paul
P
D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D000485:Allergens / Q000276:immunology*; D000818:Animals; D000830:Animals, Laboratory; D003430:Cross-Sectional Studies; D005260:Female; D006683:HLA-DQ Antigens / Q000235:genetics*; D059866:HLA-DQ beta-Chains; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006801:Humans; D006967:Hypersensitivity / Q000209:etiology*; D008297:Male; D008875:Middle Aged; D009784:Occupational Diseases / Q000209:etiology*; D051381:Rats / Q000276:immunology*
D000485:Allergens; D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; C472325:n 1 allergen, rat