title: Inhibition of conditioned stimulus pathway phosphoprotein 24 expression blocks the development of intermediate-term memory in Hermissenda.
abstract: Studies of memory consolidation have identified multiple phases or stages in the formation of memories. The multiple components of memory can be broadly divided into the three phases; short-term, intermediate-term, and long-term. Although molecular changes underlying short- and long-term memory have been examined extensively, the molecular mechanisms supporting the formation of intermediate-term memory are poorly understood. In several examples of cellular and synaptic plasticity, intermediate memory depends on translation but not transcription. One-trial conditioning in Hermissenda results in the development of intermediate memory that is associated with enhanced cellular excitability and the phosphorylation of a 24 kDa protein referred to as conditioned stimulus pathway phosphoprotein (Csp24). Using amino acid sequences derived from Csp24 peptide fragments, a full-length cDNA was cloned and shown to contain multiple beta-thymosin-like domains. The expression of Csp24 and the development of enhanced excitability, a characteristic of intermediate memory, were blocked by antisense oligonucleotide-mediated downregulation of Csp24 without affecting the induction of immediate enhanced excitability, a characteristic of short-term memory. These results demonstrate that the synthesis of Csp24 is required for the development and maintenance of intermediate memory.
authors.affiliation: Department of Neurobiology and Anatomy, University of Texas Medical School, Houston, Texas 77225, USA. terry.crow@uth.tmc.edu
authors.forename: Terry
authors.initials: T
authors.lastname: Crow
authors.forename: John B
authors.initials: JB
authors.lastname: Redell
authors.forename: Lian-Ming
authors.initials: LM
authors.lastname: Tian
authors.forename: Juan
authors.initials: J
authors.lastname: Xue-Bian
authors.forename: Pramod K
authors.initials: PK
authors.lastname: Dash
mesh_terms: D000200:Action Potentials / Q000187:drug effects / Q000502:physiology; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D016000:Cluster Analysis; D003214:Conditioning, Classical / Q000502:physiology*; D003599:Cytoskeleton / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D066298:In Vitro Techniques; D008568:Memory / Q000502:physiology*; D008840:Microfilament Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D008974:Mollusca; D009420:Nervous System; D009473:Neuronal Plasticity / Q000502:physiology; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D010750:Phosphoproteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000502:physiology; D013947:Thymosin / Q000378:metabolism
chemical_list: C474106:Csp24 protein, Hermissenda; D018076:DNA, Complementary; D008840:Microfilament Proteins; D016376:Oligonucleotides, Antisense; D010750:Phosphoproteins; D012333:RNA, Messenger; D013947:Thymosin