title: [Ulysses retrotransposon aspartate proteinase (Drosophila virilis)].
abstract: Retrotransposones are mobile genetic elements occurring in genomes of bacteria, plants or animals. Retrotransposones were found to contain nucleotide sequences encoding proteins which are homological to retroviral aspartic proteinases. Our research has been focused on Ulysses which is mobile genetic element found in Drosophila virilis. We suggested a primary structure of Ulysses proteinase using comparative analysis of amino acid sequences of retroviral proteinases and proteinases from retrotransposones. The appropriate cDNA fragment has been cloned and expressed in E. coli. The purification of recombinant protein (12 kD) has been carried out by affinity chromatography using pepstatine-agarose. The obtained protein has proteolytic activity at optimum pH 5.5 like the majority of aspartic proteinases.
authors.affiliation: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Miklukho-Maklaya st. 16/10, 117871 Russia. den@enzyme.siobc.ras.ru
authors.forename: D A
authors.initials: DA
authors.lastname: Volkov
authors.forename: L V
authors.initials: LV
authors.lastname: Savvateeva
authors.forename: N I
authors.initials: NI
authors.lastname: Dergousova
authors.forename: L D
authors.initials: LD
authors.lastname: Rumsh
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000235:genetics* / Q000302:isolation & purification; D017124:Conserved Sequence; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics* / Q000302:isolation & purification; D004926:Escherichia coli / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification; D018626:Retroelements
chemical_list: D029721:Drosophila Proteins; D011994:Recombinant Proteins; D018626:Retroelements; D016282:Aspartic Acid Endopeptidases; C475490:Ulysses retrotransposon aspartic proteinase, Drosophila
vernacular_title: Aspartatnaia proteinaza retrotranspozona Ulysses (Drosophila virilis).