Host cell caveolae act as an entry-port for group A streptococci.
This study identified caveolae as an entry port for group A streptococci into epithelial and endothelial cells. Scanning electron microscopy as well as ultrathin sections of infected cells demonstrated accumulation of small omega-shaped cavities in the host cell membrane close to adherent streptococci. During invasion, invaginations were formed that subsequently revealed intracellular compartments surrounding streptococci. Caveolin-1 was shown to be present in the membrane of invaginations and the compartment membranes. These compartments were devoid of any classic endosomal/lysosomal marker proteins and can thus be described as caveosomes. Disruption of caveolae with methyl-beta-cyclodextrin and filipin abolished host cell invasion. Importantly, streptococci inside caveosomes avoid fusion with lysosomes. Expressing of SfbI protein on the surface of the non-invasive S. gordonii resulted in identical morphological alterations on the host cell as for S. pyogenes. Incubation of HUVEC cells with purified recombinant sole SfbI protein also triggered accumulation of cavity-like structures and formation of membrane invaginations. Tagged to colloidal gold-particles, SfbI protein was shown to cluster following membrane contact. Thus, our results demonstrate that host cell caveolae initiate the invasion process of group A streptococci and that the streptococcal invasin SfbI is the triggering factor that activates the caveolae-mediated endocytic pathway.
Rohde
Manfred
M
Department of Microbial Pathogenicity and Vaccine Research, GBF-German Research Centre for Biotechnology, Mascheroder Weg 1, 38124 Braunschweig, Germany. mro@gbf.de
Müller
Ellruth
E
Chhatwal
Gursharan S
GS
Talay
Susanne R
SR
D018829:Adhesins, Bacterial / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D001422:Bacterial Adhesion; D001483:Base Sequence; D021941:Caveolae / Q000378:metabolism / Q000382:microbiology* / Q000648:ultrastructure; D051242:Caveolin 1; D022461:Caveolins / Q000378:metabolism; D002451:Cell Compartmentation; D002460:Cell Line; D002478:Cells, Cultured; D002772:Cholera Toxin / Q000378:metabolism; D003505:Cyclodextrins / Q000494:pharmacology; D004269:DNA, Bacterial / Q000235:genetics; D004730:Endothelium, Vascular / Q000378:metabolism / Q000382:microbiology / Q000648:ultrastructure; D004847:Epithelial Cells / Q000378:metabolism / Q000382:microbiology / Q000648:ultrastructure; D005372:Filipin / Q000494:pharmacology; D006801:Humans; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D013297:Streptococcus pyogenes / Q000235:genetics / Q000472:pathogenicity* / Q000648:ultrastructure; D047392:beta-Cyclodextrins
D018829:Adhesins, Bacterial; C495540:CAV1 protein, human; D051242:Caveolin 1; D022461:Caveolins; D003505:Cyclodextrins; D004269:DNA, Bacterial; D011993:Recombinant Fusion Proteins; D047392:beta-Cyclodextrins; C108732:methyl-beta-cyclodextrin; C053476:invasin, Yersinia; D005372:Filipin; D002772:Cholera Toxin