Cloning and characterization of a novel RING-B-box-coiled-coil protein with apoptotic function.
We have identified a novel RING-B-box-coiled-coil (RBCC) protein (MAIR for macrophage-derived apoptosis-inducing RBCC protein) that consists of an N-terminal RING finger, followed by a B-box zinc finger, a coiled-coil domain, and a B30.2 domain. MAIR mRNA was expressed widely in mouse tissues and was induced by macrophage colony-stimulating factor in murine peritoneal and bone marrow macrophages. MAIR protein initially showed a granular distribution predominantly in the cytoplasm. The addition of zinc to transfectants containing MAIR cDNA as part of a heavy metal-inducible vector caused apoptosis of the cells characterized by cell fragmentation; a reduction in mitochondrial membrane potential; activation of caspase-7, -8, and -9, but not caspase-3; and DNA degradation. We also found that the RING finger and coiled-coil domains were required for MAIR activity by analysis with deletion mutants.
Kimura
Fumihiko
F
Third Department of Internal Medicine, National Defense Medical College, Namiki, Tokorozawa, Saitama 359-8513, Japan.
Suzu
Shinya
S
Nakamura
Yukitsugu
Y
Nakata
Yukiko
Y
Yamada
Muneo
M
Kuwada
Naruo
N
Matsumura
Takuya
T
Yamashita
Takuya
T
Ikeda
Takashi
T
Sato
Ken
K
Motoyoshi
Kazuo
K
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D051017:Apoptosis Regulatory Proteins; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D009928:Organ Specificity; D011506:Proteins / Q000235:genetics*; D016415:Sequence Alignment; D016335:Zinc Fingers
D051017:Apoptosis Regulatory Proteins; D002352:Carrier Proteins; D011506:Proteins; C476418:TRIM35 protein, human