title: Cloning and functional characterization of human septin 10, a novel member of septin family cloned from dendritic cells.
abstract: Septins are a family of cytoskeletal proteins with GTPase activity involved in various cellular biological processes. Here, we describe the identification of septin 10, a novel septin family member from human dendritic cells. The 3018-bp full-length cDNA potentially encodes a 517-residue peptide, sharing closest homology to human septin 8 and septin 6. With a conserved GTP-binding motif at its N-terminus, septin 10 protein can bind to GTP and exert GTPase activity. Human septin 10 gene is electronically mapped to 8q11.2-12. It is ubiquitously expressed in normal tissues, with the abundant expression in heart and kidney, placenta, skeletal muscles, liver and lung, as well as various tumor cell lines. Interestingly, dendritic cells express upregulated septin 10 upon LPS-induced maturation. Based on its GFP-fusion protein, septin 10 is found to localize in cytoplasm and nucleus, with a subcellular pattern independent of the filamentous state of actin.
authors.affiliation: Institute of Immunology, Second Military Medical University, 800 Xiangyin Road, 200433, Shanghai, PR China. caoxt@public3.sta.net.cn
authors.forename: Lili
authors.initials: L
authors.lastname: Sui
authors.forename: Weiping
authors.initials: W
authors.lastname: Zhang
authors.forename: Qiuyan
authors.initials: Q
authors.lastname: Liu
authors.forename: Taoyong
authors.initials: T
authors.lastname: Chen
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.forename: Tao
authors.initials: T
authors.lastname: Wan
authors.forename: Min
authors.initials: M
authors.lastname: Yu
authors.forename: Xuetao
authors.initials: X
authors.lastname: Cao
mesh_terms: D000595:Amino Acid Sequence; D002467:Cell Nucleus / Q000737:chemistry; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000737:chemistry; D003598:Cytoskeletal Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D003713:Dendritic Cells / Q000201:enzymology*; D020558:GTP Phosphohydrolases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000096:biosynthesis; D058112:Septins; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D014018:Tissue Distribution; D015854:Up-Regulation
chemical_list: D003598:Cytoskeletal Proteins; D012333:RNA, Messenger; D020558:GTP Phosphohydrolases; C474375:SEPTIN10 protein, human; D058112:Septins