title: Analysis of 4.3 kilobases of divergent locus B of macaque retroperitoneal fibromatosis-associated herpesvirus reveals a close similarity in gene sequence and genome organization to Kaposi's sarcoma-associated herpesvirus.
abstract: We previously identified retroperitoneal fibromatosis-associated herpesvirus (RFHV) as a simian homolog of Kaposi's sarcoma-associated herpesvirus (KSHV) in a fibroproliferative malignancy of macaques that has similarities to Kaposi's sarcoma. In this report, we cloned 4.3 kb of divergent locus B (DL-B) flanking the DNA polymerase gene from two variants of RFHV from different species of macaque with a consensus degenerate hybrid oligonucleotide primer approach. Within the DL-B region of RFHV, viral homologs of the cellular interleukin-6, dihydrofolate reductase, and thymidylate synthase genes were identified, along with a homolog of the gammaherpesvirus open reading frame (ORF) 10. In addition, a homolog of the KSHV ORF K3, the modulator of immune recognition-1, was identified. Our data show a close similarity in sequence conservation, gene content, and genomic structure between RFHV and KSHV which strongly supports the grouping of these viral species within the same RV-1 rhadinovirus lineage and the hypothesis that RFHV is the macaque homolog of KSHV.
authors.affiliation: Department of Pathobiology, School of Public Health and Community Medicine, University of Washington, Seattle 98195, USA. trose@u.washington.edu
authors.forename: Timothy M
authors.initials: TM
authors.lastname: Rose
authors.forename: Jonathan T
authors.initials: JT
authors.lastname: Ryan
authors.forename: Emily R
authors.initials: ER
authors.lastname: Schultz
authors.forename: Brian W
authors.initials: BW
authors.lastname: Raden
authors.forename: Che-Chung
authors.initials: CC
authors.lastname: Tsai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004259:DNA-Directed DNA Polymerase / Q000235:genetics; D019143:Evolution, Molecular; D006566:Herpesviridae Infections / Q000662:veterinary / Q000821:virology; D019288:Herpesvirus 8, Human / Q000737:chemistry / Q000145:classification* / Q000235:genetics*; D006801:Humans; D008253:Macaca mulatta; D008254:Macaca nemestrina; D008969:Molecular Sequence Data; D008992:Monkey Diseases / Q000821:virology; D010802:Phylogeny; D012185:Retroperitoneal Fibrosis / Q000662:veterinary / Q000821:virology; D012186:Retroperitoneal Neoplasms / Q000662:veterinary / Q000821:virology; D018144:Rhadinovirus / Q000737:chemistry / Q000145:classification* / Q000235:genetics*; D012514:Sarcoma, Kaposi / Q000821:virology; D017422:Sequence Analysis, DNA; D014412:Tumor Virus Infections / Q000662:veterinary / Q000821:virology
chemical_list: D004259:DNA-Directed DNA Polymerase