Endosperm degradation during seed development of Echinocystis lobata (Cucurbitaceae) as a manifestation of programmed cell death (PCD) in plants.
Programmed cell death (PCD) is an active, genetically controlled process that ultimately leads to elimination of unnecessary or damaged cells from multicellular organism. It occurs during normal growth and development or in response to a variety of environmental triggers and is indispensable for survival of the organism. In Echinocystis lobata the endosperm, an ephemeral tissue in angiosperm plants, undergoes distinct cytological, physiological and molecular changes during seed development and maturation. As a result, mature seeds are deprived of this tissue. The endosperm was analyzed at the consecutive stages of seed development. The morphological changes of cells were studied at light and electron microscope levels. In this paper we report that endosperm cells undergo morphological and biochemical changes characteristic of apoptosis, a particular type of PCD, i.e. cell shrinkage, chromatin condensation, nuclear fragmentation, and cytoplasm degradation, while the ultrastructure of mitochondria seems to be less changed. Furthermore, the progression of DNA degradation has been shown by agarose gel electrophoresis (ladder pattern of DNA fragmentseparation), TUNEL and comet assay. It isconcluded that during seed maturation, endosperm degradation process is accompanied by typical PCD-related changes of cell morphology and internucleosomal DNA cleavage.
Wojciechowska
Marzena
M
Department of Cytogenetics and Plant Molecular Biology, University of Lódź, Poland.
Olszewska
Maria J
MJ
D017209:Apoptosis; D019662:Cucurbitaceae / Q000196:embryology* / Q000235:genetics; D053938:DNA Fragmentation; D018744:DNA, Plant / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000196:embryology* / Q000378:metabolism* / Q000648:ultrastructure
D018744:DNA, Plant