title: Purification and some properties of lysostaphin, a glycylglycine endopeptidase from the culture liquid of Staphylococcus simulans biovar staphylolyticus.
abstract: This work presents a method for purification of lysostaphin, a glycylglycine endopeptidase, from the culture liquid of S. simulans biovar staphylolyticus to homogeneity in a few steps. The method includes ultrafiltration and ion-exchange and hydrophobic chromatographies. The enzyme was isolated in preparative amounts with the yield of 51%. Some physical and chemical properties of the enzyme are described.
authors.affiliation: State Research Center for Applied Microbiology, Ministry of Health of Russian Federation, Obolensk, Moscow Region, 142279, Russia. center@gncpm.serpukhov.su
authors.forename: T V
authors.initials: TV
authors.lastname: Fedorov
authors.forename: V I
authors.initials: VI
authors.lastname: Surovtsev
authors.forename: V Z
authors.initials: VZ
authors.lastname: Pletnev
authors.forename: M A
authors.initials: MA
authors.lastname: Borozdina
authors.forename: V V
authors.initials: VV
authors.lastname: Gusev
mesh_terms: D017077:Culture Media, Conditioned / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D057927:Hydrophobic and Hydrophilic Interactions; D008248:Lysostaphin / Q000737:chemistry* / Q000302:isolation & purification*; D013210:Staphylococcus / Q000201:enzymology*
chemical_list: D017077:Culture Media, Conditioned; D008248:Lysostaphin