The influence of epoxomicin, inhibitor of proteasomal proteolytic activity, on spermiogenesis in Chara vulgaris.
The influence of 48-h treatment with epoxomicin, an inhibitor of proteolytic activity of proteasomes, at the concentration 10 microM, on spermiogenesis in algae Chara vulgaris was examined. In the presence of the inhibitor, the frequency of early spermiogenesis phases significantly increased, the number of spermatids in mid-phases decreased and disappearance of late phases was observed. A hypothesis has been put forward that epoxomicin stops spermiogenesis during the period of preparation to further deep reorganisation of spermatids by blocking proteolysis of short-lived regulatory proteins which are responsible among others for triggering the exchange of nucleohistones into nucleoprotamines.
Kwiatkowska
Maria
M
Department of Cytophysiology, University of Lódź, Poland. kwiat@biol.uni.lodz.pl
Wojtczak
Agnieszka
A
Popłońska
Katarzyna
K
Teodorczyk
Marta
M
D002454:Cell Differentiation / Q000187:drug effects; D003546:Cysteine Endopeptidases / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D056890:Eukaryota / Q000502:physiology*; D009097:Multienzyme Complexes / Q000187:drug effects; D009842:Oligopeptides / Q000494:pharmacology*; D046988:Proteasome Endopeptidase Complex; D013091:Spermatogenesis / Q000187:drug effects / Q000502:physiology*; D013997:Time Factors
D004791:Enzyme Inhibitors; D009097:Multienzyme Complexes; D009842:Oligopeptides; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex; C078846:epoxomicin