title: [Effect of tumor necrosis factor-alpha on differentiation of mesencephalic neural stem cells and proliferation of oligodendrocytes in the rat].
abstract: To observe the influence of tumor necrosis factor-alpha (TNF-alpha) on differentiation of rat mesencephalic neural stem cells (NSCs), the numbers of neurons, astrocytes and oligodendrocytes generated from NSCs were analyzed after differentiation for 3 days by using immunocytochemistry technique. The results show that: (1) TNF-alpha enhanced the proportions of neurons and oligodendrocytes in progeny of NSCs; and (2) TNF-alpha induced the proliferation of oligodendrocytes derived from NSCs, but the proliferation of astrocytes was not influenced by TNF-alpha. We conclude that the TNF-alpha could influence the application of NSCs.
authors.affiliation: Department of Neurobiology, Second Military Medical University, Shanghai, PR China. loushjie@hotmail.com
authors.forename: Shu-Jie
authors.initials: SJ
authors.lastname: Lou
authors.forename: Ping
authors.initials: P
authors.lastname: Gu
authors.forename: He
authors.initials: H
authors.lastname: Xu
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Xu
authors.forename: Ming-Wei
authors.initials: MW
authors.lastname: Wang
authors.forename: Cheng
authors.initials: C
authors.lastname: He
authors.forename: Chang-Lin
authors.initials: CL
authors.lastname: Lu
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000166:cytology; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D008636:Mesencephalon / Q000166:cytology*; D058953:Neural Stem Cells / Q000166:cytology*; D009474:Neurons / Q000166:cytology; D009836:Oligodendroglia / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*
chemical_list: D014409:Tumor Necrosis Factor-alpha