Colocalization of estrogen beta-receptor messenger RNA with orphanin FQ, vasopressin and oxytocin in the rat hypothalamic paraventricular and supraoptic nuclei.
The functional significance of the novel estrogen receptor beta in brain areas that exclusively contain the ERbeta receptor subtype such as the paraventricular (PVN) and the supraoptic (SON) nuclei of the hypothalamus is not yet fully understood. The present study attempts to characterize the peptidergic nature of the ERbeta-containing neuronal population in the PVN and the SON using the double in situ histochemistry method in the female rat. Using this method, the ERbeta mRNA coexpressions with the novel opioid neuropeptide (orphanin FQ and its receptor ORL1) mRNA in addition to the previously reported neuropeptide (arginine vasopressin-AVP, oxytocin-OXY, corticotropin releasing hormone-CRH, enkephalin-ENK) mRNAs were assessed. In the PVN, roughly half of the ERbeta expression was colocalized with the prepro-orphanin FQ mRNA, which was comparable to the colocalization observed between the ERbeta and AVP mRNAs in the same region. In addition, there was 20% overlap between the ERbeta and ORL1 receptor mRNAs, and 10% overlap between the ERbeta and OXY mRNAs in the PVN. By contrast, the coexpression between the prepro-orphanin FQ and ERbeta mRNAs was less striking in the SON. Potential interactions between the ERbeta and the well-characterized AVP-OXY neurosecretory system as well as the novel OFQ-ORL1 opioid neuropeptide system may provide new leads for the functional significance of ERbeta, specifically in stress/autonomic responses.
Isgor
Ceylan
C
Mental Health Research Institute, The University of Michigan, Ann Arbor, MI 48109-0720, USA. isgor@umich.edu
Shieh
Kun-Ruey
KR
Akil
Huda
H
Watson
Stanley J
SJ
D000818:Animals; D047629:Estrogen Receptor beta; D018846:Excitatory Amino Acids / Q000819:agonists; D005260:Female; D007032:Hypothalamus, Anterior / Q000502:physiology*; D018847:Opioid Peptides; D010121:Oxytocin / Q000032:analysis / Q000494:pharmacology; D010286:Paraventricular Hypothalamic Nucleus / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011960:Receptors, Estrogen / Q000235:genetics* / Q000502:physiology; D011957:Receptors, Opioid / Q000819:agonists / Q000032:analysis; D013315:Stress, Psychological / Q000503:physiopathology; D014667:Vasopressins / Q000032:analysis / Q000494:pharmacology; D000094942:Nociceptin Receptor; D000097629:Nociceptin
D047629:Estrogen Receptor beta; D018846:Excitatory Amino Acids; D018847:Opioid Peptides; D010121:Oxytocin; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D011957:Receptors, Opioid; D014667:Vasopressins; D000094942:Nociceptin Receptor; D000097629:Nociceptin; C000722285:Oprl1 protein, rat