title: Reproduction of hydropericardium syndrome in three-week-old cyclophosphamide-treated specific-pathogen-free chickens by adenoviruses from inclusion body hepatitis.
abstract: The pathogenicity of serotype 8 group I avian adenovirus (GIAAV) strains (TR630 and Saga97 strains) from inclusion body hepatitis (IBH) against cyclophosphamide (CY)-treated 3-wk-old specific-pathogen-free (SPF) chickens was examined. SPF chickens were inoculated intramuscularly with 10(7) plaque-forming units of viruses. Both strains from IBH could produce hydropericardium and mortality in CY-treated chickens as hydropericardium syndrome (HPS) that serotype 4 GIAAV strains cause, although they could not induce either hydropericardium or mortality in nontreated chickens. Histologically, hepatocytic necrosis with intranuclear inclusions, pancreatic acinar necrosis with intranuclear inclusions, and epicardial edema were seen in CY-treated chickens inoculated with GIAAV from IBH. Immunohistochemically, these inclusions were positive against GIAAV antigen. There were neither histologic lesions nor positive reactions against GIAAV antigen in nontreated chickens inoculated with GIAAV from IBH. From the present findings, pathogenic characteristics of IBH strains and HPS strains in the chickens were essentially the same.
authors.affiliation: National Institute of Animal Health, Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Kikuyasu
authors.initials: K
authors.lastname: Nakamura
authors.forename: Takeshi
authors.initials: T
authors.lastname: Shoyama
authors.forename: Masaji
authors.initials: M
authors.lastname: Mase
authors.forename: Tadao
authors.initials: T
authors.lastname: Imada
authors.forename: Manabu
authors.initials: M
authors.lastname: Yamada
mesh_terms: D000257:Adenoviridae Infections / Q000473:pathology / Q000662:veterinary; D000375:Aging / Q000502:physiology; D000818:Animals; D000259:Aviadenovirus / Q000145:classification / Q000472:pathogenicity*; D002645:Chickens / Q000821:virology*; D003520:Cyclophosphamide / Q000494:pharmacology*; D006331:Heart Diseases / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D006524:Hepatitis, Viral, Animal / Q000473:pathology / Q000821:virology*; D007150:Immunohistochemistry; D007166:Immunosuppressive Agents / Q000494:pharmacology; D002479:Inclusion Bodies / Q000821:virology*; D009206:Myocardium / Q000473:pathology; D010496:Pericardium / Q000187:drug effects / Q000473:pathology*; D011201:Poultry Diseases / Q000473:pathology / Q000821:virology*; D013047:Specific Pathogen-Free Organisms; D013577:Syndrome
chemical_list: D007166:Immunosuppressive Agents; D003520:Cyclophosphamide