Rice UV-damaged DNA binding protein homologues are most abundant in proliferating tissues.
Ultraviolet-damaged DNA binding protein (UV-DDB) is an important factor involved in DNA repair. To study the role of UV-DDB, we attempted to obtain the cDNA and the protein of a plant UV-DDB. We succeeded in isolating both genes for UV-DDB subunits from rice (Oryza sativa cv. Nipponbare), designated as OsUV-DDB1 and OsUV-DDB2. OsUV-DDB2 (65 kDa) was much larger than human UV-DDB2, but immunoprecipitation and gel mobility shift assay suggested that OsUV-DDB2 is a plant counterpart of UV-DDB2. The transcripts were expressed in proliferating tissues such as the meristem, but were detected at only low levels in the mature leaves, although the leaves are strongly exposed to UV. These transcripts were induced in the meristem after UV-irradiation. The expression levels of OsUV-DDB were significantly reduced when cell proliferation was temporarily halted. These results indicated that the level of OsUV-DDB expression is correlated with cell proliferation, and its expression may be required mostly for DNA repair in DNA replication.
Ishibashi
Toyotaka
T
Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-0022, Japan.
Kimura
Seisuke
S
Yamamoto
Taichi
T
Furukawa
Tomoyuki
T
Takata
Kei-ichi
K
Uchiyama
Yukinobu
Y
Hashimoto
Junji
J
Sakaguchi
Kengo
K
D000595:Amino Acid Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D018519:Meristem / Q000235:genetics* / Q000528:radiation effects; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D018520:Plant Shoots / Q000235:genetics* / Q000528:radiation effects; D011485:Protein Binding; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013997:Time Factors; D014466:Ultraviolet Rays
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D020033:Protein Isoforms; D018749:RNA, Plant