The absolute number of peripheral blood CD34+ cells predicts a timing for apheresis and progenitor cell yield in patients with hematologic malignancies and solid tumors.
Retrospective analysis was conducted in 51 autologous peripheral blood progenitor cell (PBPC) collections using the Spectra AutoPBSC System from patients with hematologic malignancies and solid tumors to study the predictive value of CD34+ cell counts in the peripheral blood for the yield of CD34+ cells in the apheresis product. The correlation coefficients for CD34+ cells microL(-1) of peripheral blood with CD34+ cell yield (x 10(6) kg(-1) of body weight and x 10(5) kg(-1) of body weight L(-1) of blood processed) were 0.903 and 0.778 (n=51 collections), respectively. Products collected from patients with CD34+ cell counts below 15 microL(-1) in the peripheral blood contained a median of 0.49 x 10(6) CD34+ cells kg(-1) (range: 0.05-2.55), whereas those with CD34+ cell counts more than 15 microL(-1) contained a median of 3.72 x 10(6) CD34+ cells kg(-1) (range: 1.06-37.57). From these results, a number of at least 15 CD34+ cells microL(-1) in the peripheral blood ensured a minimum yield of 1 x 10(6) CD34+ cells kg(-1) as obtained by a single apheresis procedure. The number of CD34+ cells in the peripheral blood can be used as a good predictor for timing of apheresis and estimating PBPC yield. With regard to our results, apheresis with a possibly poor efficiency should be avoided because the collection procedure is time-consuming and expensive.
Kudo
Yoshinori
Y
Division of Blood Transfusion, Tohoku University Hospital, Sendai 980-8574, Japan.
Minegishi
Masayoshi
M
Saito
Naoko
N
Itoh
Tsuneo
T
Fushimi
Junko
J
Takahashi
Hiroyuki
H
Suzuki
Akira
A
Narita
Ayuko
A
Sato
Yuko
Y
Miura
Junko
J
Yoshihara
Kazue
K
Meguro
Kuniaki
K
Imaizumi
Masue
M
Sato
Makoto
M
Murakawa
Yasuko
Y
Tsuchiya
Shigeru
S
D000293:Adolescent; D000328:Adult; D018952:Antigens, CD34 / Q000096:biosynthesis / Q000097:blood*; D001781:Blood Component Removal / Q000379:methods*; D001835:Body Weight; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry; D019337:Hematologic Neoplasms / Q000097:blood* / Q000378:metabolism; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D013234:Stem Cells / Q000378:metabolism*; D013997:Time Factors
D018952:Antigens, CD34