title: Failure of a recombinant Schistosoma bovis-derived glutathione S-transferase to protect cattle against experimental Fasciola hepatica infection.
abstract: The potential of a recombinant Schistosoma bovis 28-kDa glutathione S-transferase (rSb28GST) to protect cattle against Fasciola hepatica was tested in a vaccination trial. Thirty two calves were randomly divided into four groups of eight animals. Calves of the three vaccine groups received two intramuscular injections at 3 weeks interval, of 0.250mg rSb28GST in either aluminium hydroxide (Al(OH)(3)), Quil A, or PBS emulsified in an equal volume of Freund's complete adjuvant (FCA).Animals of the control group received injections of Al(OH)(3)/PBS only. All animals were challenged orally with a total of 360 metacercariae of F. hepatica, spread over 6 weeks. All groups of vaccinated animals produced measurable IgG antibody titers to rSb28GST after vaccination. Animals immunised with FCA adjuvanted vaccine had the highest and more durable antibody titers and only sera from this group recognised an approximately 24kDa protein band from F. hepatica, that is thought to be a F. hepatica GST. Despite a good antibody response differences in cumulative faecal egg output between the groups were not statistically significant. In addition, no significant difference was found between groups in terms of total worm numbers or percentage of immature flukes recovered at necropsy. In conclusion, the recombinant S. bovis 28kDa GST was not found to adequately protect cattle against experimental F. hepatica challenge, using either aluminium hydroxide, Quil A or FCA as adjuvant.
authors.affiliation: Inserm U547, Institut Pasteur de Lille, Rue du Prof Calmette, 59019 Lille, France.
authors.forename: J
authors.initials: J
authors.lastname: De Bont
authors.forename: E
authors.initials: E
authors.lastname: Claerebout
authors.forename: G
authors.initials: G
authors.lastname: Riveau
authors.forename: A M
authors.initials: AM
authors.lastname: Schacht
authors.forename: K
authors.initials: K
authors.lastname: Smets
authors.forename: G
authors.initials: G
authors.lastname: Conder
authors.forename: D A
authors.initials: DA
authors.lastname: Brake
authors.forename: A
authors.initials: A
authors.lastname: Capron
authors.forename: J
authors.initials: J
authors.lastname: Vercruysse
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000469:parasitology* / Q000517:prevention & control*; D004802:Eosinophilia / Q000276:immunology; D005210:Fasciola hepatica / Q000254:growth & development / Q000276:immunology*; D005211:Fascioliasis / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control / Q000662:veterinary*; D005243:Feces / Q000469:parasitology; D005260:Female; D005982:Glutathione Transferase / Q000276:immunology* / Q000494:pharmacology; D007114:Immunization / Q000662:veterinary*; D010270:Parasite Egg Count / Q000662:veterinary; D011897:Random Allocation; D011994:Recombinant Proteins / Q000276:immunology / Q000494:pharmacology; D012547:Schistosoma / Q000201:enzymology / Q000276:immunology*; D005723:gamma-Glutamyltransferase / Q000097:blood
chemical_list: D000276:Adjuvants, Immunologic; D000909:Antibodies, Helminth; D011994:Recombinant Proteins; D005723:gamma-Glutamyltransferase; D005982:Glutathione Transferase