[Study of functional activity of components and factors of the human complement system].
Development suitable for clinical researches of hemolytic methods of determination of functional activity of the first components of a complement has allowed to show diagnostic value of testing activity of complement components in comparison with their contents as antigens. It has predetermined necessity for building modern ELISA tests-systems for quantitative determination of functional activity of complement components. Such methods built for the first time allow to determine activity of components C1q, C2, C3, C4 (and a ratio of isotypes C4A and C4B), C1-inhibitor, factors B and D. Addition of these tests-systems ELISA systems for quantitative determination of components, and in case of C1-inhibitor of presence IgG, IgA and IgM autoantibodies against C1-inhibitor frames opportunities of an evaluation complement status of the patient, hereditary predisposition to such diseases as a stomach ulcer, the glaucoma, a clamidiosis, bacteroidosis, allows to carry out differential diagnostics of angioedema. Inhibition of covalent linkage C4b or C3b various endogenic and exogenous effectors during formation C3- and C5-convertases allows to understand processes of a regulation of a homeostasis, and also the mechanism of action of drugs.
Kozlov
L V
LV
G.N. Gabrichevsky Moscow Scientific Research Institute of Epidemiology and Microbiology, 125212 Moscow, Street of Admiral Makarova. L.V.Kozlov@mtu-net.ru
D001323:Autoantibodies / Q000097:blood; D019411:Clinical Laboratory Techniques; D003167:Complement Activation; D003165:Complement System Proteins / Q000032:analysis* / Q000276:immunology; D006403:Hematologic Tests / Q000379:methods; D006461:Hemolysis; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D011933:Reagent Kits, Diagnostic
D001323:Autoantibodies; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011933:Reagent Kits, Diagnostic; D003165:Complement System Proteins
Issledovanie funktsional'noĭ aktivnosti komponentov i faktorov sistemy komplementa cheloveka.