title: Determining hepatitis C genotype by analyzing the sequence of the NS5b region.
abstract: Assays to determine the hepatitis C virus (HCV) genotype have recently become useful for clinical decision making and may be suitable for epidemiological investigations, such as identifying HCV outbreaks in a given population. Molecular assays are the most common diagnostic tools for HCV genotyping. This study compares two genome typing assays, one, the Trugene 5'NC genotyping kit, uses the sequence of the 5' non-coding (5'NC) region and the other, a non-commercial assay, uses the non-structural 5b (NS5b) region. Serum samples from 203 chronically HCV-infected patients were tested. The 5'NC and the NS5b assays were both very effective in identifying the genotype (99 and 98.5%) and the results with the two methods were always concordant for the genotype. The NS5b analysis permitted the identification of the subtype in all samples, whereas the 5'NC region assay did not in 33% of samples. The NS5b analysis showed that one patient had a mixed infection with HCV subtypes 1a and 2c, while the 5'NC assay did not. It is concluded that phylogenetic analysis using both the 5'NC and the NS5b regions are reliable and convenient methods for HCV typing in clinical practice. But analysis of the NS5b region may be more useful for tracing the source of an HCV infection.
authors.affiliation: Service de Virologie, Hôpital Purpan, CHU, Toulouse, France. saune.k@chu-toulouse.fr
authors.forename: K
authors.initials: K
authors.lastname: Sandres-Sauné
authors.forename: P
authors.initials: P
authors.lastname: Deny
authors.forename: C
authors.initials: C
authors.lastname: Pasquier
authors.forename: V
authors.initials: V
authors.lastname: Thibaut
authors.forename: G
authors.initials: G
authors.lastname: Duverlie
authors.forename: J
authors.initials: J
authors.lastname: Izopet
mesh_terms: D020121:5' Untranslated Regions / Q000737:chemistry*; D005838:Genotype; D016174:Hepacivirus / Q000145:classification* / Q000235:genetics; D010802:Phylogeny; D017361:Viral Nonstructural Proteins / Q000737:chemistry*; D012324:RNA-Dependent RNA Polymerase
chemical_list: D020121:5' Untranslated Regions; D017361:Viral Nonstructural Proteins; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase