The heparan sulfate proteoglycan gene polymorphism: association with type 2 diabetic nephropathy in Chinese.
The objective of this study was to investigate the association between the heparan sulfate proteoglycan (HSPG) gene G/T polymorphism and diabetic nephropathy in type 2 diabetes in Chinese. The HSPG gene polymorphism (G/T) was determined using polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) in 290 Chinese type 2 diabetes mellitus patients comprising 77 cases without nephropathy and 213 cases with nephropathy with either proteinuria (163) or renal insufficiency (50) and a control group of 190 non-diabetics. A significant increase in the frequencies of the T allele and TT + TG genotype was observed in subjects with diabetic renal insufficiency as compared with diabetic subjects without nephropathy and those with diabetic proteinuria (for the T allele: Fisher's two-tailed exact p was 0.015 and 0.002, respectively; for the TT + TG genotype; both p values were < 0.001). Diabetic subjects without nephropathy and those with albuminuria carrying the T allele had odds ratios for the development of renal insufficiency of 1.92 (95% CI, 1.12-3.30) and 2.09 (95% CI, 1.29-3.38), respectively, as compared to non-carriers. The T allele of the HSPG gene BamHI polymorphism located in intron 6 may be a risk factor for the development of renal insufficiency in type 2 diabetes mellitus for Chinese.
Liu
Limei
L
Shanghai Diabetes Institute, Shanghai No. 6 People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China. liulimei2001@hotmail.com
Xiang
Kunsan
K
Zheng
Taishan
T
Zhang
Rong
R
Li
Ming
M
Wang
Yanqing
Y
Lu
Huijuan
H
Li
Jie
J
D000368:Aged; D000419:Albuminuria / Q000235:genetics; D000483:Alleles; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D002681:China; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D003928:Diabetic Nephropathies / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005819:Genetic Markers; D019812:Heparan Sulfate Proteoglycans / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011507:Proteinuria / Q000235:genetics; D051437:Renal Insufficiency / Q000235:genetics; D012307:Risk Factors
D005819:Genetic Markers; D019812:Heparan Sulfate Proteoglycans