Isolation and characterization of Saccharomyces cerevisiae mutants with a different degree of resistance to killer toxins K1 and K2.
Killer toxin K1 of Saccharomyces cerevisiae kills sensitive cells of the same species by disturbing the ion gradient across the plasma membrane after binding to the receptor at cell wall beta-1,6-glucan. Killer protein K2 is assumed to act by a similar mechanism. To identify the putative plasma membrane receptors for both toxins we mutagenized three sensitive S. cerevisiae strains and searched for clones with killer-resistant spheroplasts. The well diffusion assay identified three phenotypically different groups of clones: clones resistant simultaneously to both toxins, clones with lowered sensitivity to only K1 toxin and those with strongly lowered sensitivity to K2 and partially lowered sensitivity to K1 toxin. These phenotypes are controlled by recessive mutations that belong to at least four different complementation groups. This indicates certain differences at the level of interaction of K1 and K2 toxin with sensitive cells.
Flegelová
Hana
H
Charles University, Faculty of Science, Department of Genetics and Microbiology, Vinicná 5, 128 44 Praha 2, Czech Republic. hanafle@natur.cuni.cz
Novotná
Drahomíra
D
Vojtísková
Katerina
K
Janderová
Blanka
B
D004352:Drug Resistance, Microbial / Q000235:genetics*; D005816:Genetic Complementation Test; D055646:Killer Factors, Yeast; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D009154:Mutation; D009183:Mycotoxins / Q000378:metabolism / Q000494:pharmacology*; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013104:Spheroplasts / Q000187:drug effects / Q000235:genetics / Q000378:metabolism
C089359:K1 killer toxin; D055646:Killer Factors, Yeast; D008565:Membrane Proteins; D009183:Mycotoxins; D011956:Receptors, Cell Surface