title: The roles of bone morphogenetic protein (BMP) 12 in stimulating the proliferation and matrix production of human patellar tendon fibroblasts.
abstract: Recombinant human (rh) bone morphogenetic protein 12 (BMP12) is proved to induce the formation of tendon and ligament tissues in animal experiments. But the roles of BMP12 on tissue regeneration in human tendons remain unexplored. In the present study, healthy human patellar tendon samples were collected for histological examination and preparation of tendon fibroblast culture. Immunohistochemical staining showed that BMP12 was detected on healthy patellar tendon samples, only located on active tenoblasts and perivascular mesenchymal cells but not in interstitial tenocytes. The expression of PCNA and procollagen type I also exhibited a similar distribution. It indicates that BMP12 may be involved in matrix remodeling process in adult tissues. In vitro studies showed that rhBMP12 could increase proliferation of tendon fibroblasts and increase the gene expression of procollagen type I and type III, but decrease the gene expression of decorin in tendon fibroblasts culture. Our findings suggest that BMP12 may play a role in early phases of tissue regeneration in tendons.
authors.affiliation: Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong, China
authors.forename: Sai Chuen
authors.initials: SC
authors.lastname: Fu
authors.forename: Yim Ping
authors.initials: YP
authors.lastname: Wong
authors.forename: Barbara Pui
authors.initials: BP
authors.lastname: Chan
authors.forename: Hon Man
authors.initials: HM
authors.lastname: Pau
authors.forename: Yau Chuk
authors.initials: YC
authors.lastname: Cheuk
authors.forename: Kwong Man
authors.initials: KM
authors.lastname: Lee
authors.forename: Kai-Ming
authors.initials: KM
authors.lastname: Chan
mesh_terms: D000293:Adolescent; D000328:Adult; D058578:Biglycan; D019485:Bone Morphogenetic Proteins / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division / Q000502:physiology*; D002478:Cells, Cultured; D058575:Decorin; D005109:Extracellular Matrix / Q000378:metabolism*; D016326:Extracellular Matrix Proteins; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology*; D055412:Growth Differentiation Factors; D006801:Humans; D008297:Male; D017847:Patellar Ligament / Q000166:cytology / Q000378:metabolism*; D011347:Procollagen / Q000235:genetics / Q000378:metabolism; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011509:Proteoglycans / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016212:Transforming Growth Factor beta
chemical_list: C546884:BGN protein, human; D058578:Biglycan; D019485:Bone Morphogenetic Proteins; C546878:DCN protein, human; D058575:Decorin; D016326:Extracellular Matrix Proteins; C499470:GDF7 protein, human; D055412:Growth Differentiation Factors; D011347:Procollagen; D018809:Proliferating Cell Nuclear Antigen; D011509:Proteoglycans; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C086559:growth differentiation factor 7