title: Multiple levels of interactions within the tetraspanin web.
abstract: The tetraspanin web refers to a network of molecular interactions involving tetraspanins and other molecules. Inside the tetraspanin web, small primary complexes containing only one tetraspanin and one specific partner molecule such as CD151/alpha3beta1 integrin and CD9/CD9P-1 (FPRP) can be observed under particular conditions. Here we demonstrate that when cells are lysed with Brij97, the tetraspanins CD151 and CD9 allow and/or stabilize the interaction of their partner molecules with other tetraspanins and that their two partners associate under conditions maintaining tetraspanin/tetraspanin interactions. The tetraspanins were also found to partition into a detergent-resistant membrane environment to which the integrin alpha3beta1 was relocalized upon expression of CD151.
authors.affiliation: INSERM U268, Institut André Lwoff, Hôpital Paul Brousse, 94807, Villejuif Cedex, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Charrin
authors.forename: Serge
authors.initials: S
authors.lastname: Manié
authors.forename: Martine
authors.initials: M
authors.lastname: Billard
authors.forename: Leonie
authors.initials: L
authors.lastname: Ashman
authors.forename: Denis
authors.initials: D
authors.lastname: Gerlier
authors.forename: Claude
authors.initials: C
authors.lastname: Boucheix
authors.forename: Eric
authors.initials: E
authors.lastname: Rubinstein
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D002460:Cell Line; D006224:Cricetinae; D003902:Detergents / Q000737:chemistry; D006801:Humans; D039201:Integrin alpha3beta1 / Q000032:analysis / Q000378:metabolism; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins / Q000378:metabolism; D021962:Membrane Microdomains / Q000737:chemistry; D008565:Membrane Proteins / Q000378:metabolism; D008954:Models, Biological; D009363:Neoplasm Proteins / Q000378:metabolism; D060265:Tetraspanin 25; D060225:Tetraspanin 28; D060245:Tetraspanin 29
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; C554894:CD53 protein, human; C554798:CD81 protein, human; C554869:CD9 protein, human; D002352:Carrier Proteins; D003902:Detergents; D039201:Integrin alpha3beta1; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D009363:Neoplasm Proteins; C486331:PTGFRN protein, human; D060265:Tetraspanin 25; D060225:Tetraspanin 28; D060245:Tetraspanin 29