title: Isolation and characterisation of the phospholipase B gene of Cryptococcus neoformans var. gattii.
abstract: Cryptococcus neoformans var. gattii (serotypes B and C) is a human pathogen, ecologically, biochemically, clinically and genetically different from C. neoformans var. grubii (serotype A) and C. neoformans var. neoformans (serotype D). The phospholipase B (PLB1) gene from serotypes B and C was isolated and characterised. It resembled the serotype A and D genes, with an overall sequence homology of more than 85%. The respective open reading frames were 2236 bp (serotype B) and 2239 bp (serotype C) in length. Each contained six introns and encoded a 68-kDa protein destined for secretion. PLB1 was located on the second smallest chromosome in both serotypes. Gene expression, measured as mRNA, was not regulated by temperature, pH or exogenous nutrients.
authors.affiliation: Molecular Mycology Laboratory, CIDM, Westmead Hospital, ICPMR, Level 3 Room 3114A, Darcy Road, NSW 2145, Australia.
authors.forename: G Nicolas
authors.initials: GN
authors.lastname: Latouche
authors.forename: Tania C
authors.initials: TC
authors.lastname: Sorrell
authors.forename: Wieland
authors.initials: W
authors.lastname: Meyer
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003455:Cryptococcus neoformans / Q000201:enzymology / Q000235:genetics*; D004271:DNA, Fungal / Q000032:analysis; D005798:Genes, Bacterial; D057927:Hydrophobic and Hydrophilic Interactions; D008245:Lysophospholipase / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D012703:Serotyping
chemical_list: D004271:DNA, Fungal; D008245:Lysophospholipase