title: Detecting clonal rearrangement in non-Hodgkin's lymphomas in Taiwan by polymerase chain reaction.
abstract: The detection of monoclonal expansions of the immunoglobulin heavy chain (IgH) or the T-cell receptor-gamma (TCRgamma) chain genes is an important supplement for the diagnosis of the non-Hodgkin's lymphomas (NHLs). Detection of monoclonality by polymerase chain reaction (PCR) method has offered an efficient approach for rapid diagnosis and monitoring of the therapeutic effects. Here we conducted a retrospective PCR clonality study on 49 cases of NHLs including 23 B-cell lymphomas (BCLs), 20 peripheral T-cell lymphomas (PTCLs), 6 natural killer (NK)/T-cell lymphomas and 3 reactive lymphoid tissues from southern Taiwan. Genomic DNAs from paraffin sections were extracted and analyzed by the IgH- and TCR-specific PCR reactions. The results showed that 20 of 23 (87.5%) BCLs exhibited IgH gene rearrangements and were all germline for TCRgamma. 15 of 20 (75.0%) PTCLs exhibited TCRgamma gene rearrangements while 1 case (5%) was positive for IgH gene rearrangement. The 6 NK/T-cell lymphomas and 3 reactive lymphoid tissues were all germline for either IgH or TCRgamma genes. Our results were similar to other Western reports in terms of sensitivity and cell-lineage specificity. This is the first large series of PCR clonality study of IgH and TCRgamma gene rearrangements on NHLs from Taiwan. We have confirmed that this rapid method is a sensitive diagnostic tool for NHLs.
authors.affiliation: Department of Pathology, Chi-Mei Foundation Hospital, Tainan, Taiwan.
authors.forename: Shih-Sung
authors.initials: SS
authors.lastname: Chuang
authors.forename: Ching-Nan
authors.initials: CN
authors.lastname: Lin
authors.forename: Fan-Ching
authors.initials: FC
authors.lastname: Shen
authors.forename: Pei-Ju
authors.initials: PJ
authors.lastname: Liao
authors.forename: Yung-Liang
authors.initials: YL
authors.lastname: Liao
authors.forename: Julia Hueimei
authors.initials: JH
authors.lastname: Chang
authors.forename: Yi-Chang
authors.initials: YC
authors.lastname: Tsai
authors.forename: Chi-Yi
authors.initials: CY
authors.lastname: Cho
authors.forename: Wenya
authors.initials: W
authors.lastname: Huang
mesh_terms: D002999:Clone Cells / Q000473:pathology; D015321:Gene Rearrangement; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D005803:Genes, Immunoglobulin; D006801:Humans; D016393:Lymphoma, B-Cell / Q000235:genetics / Q000473:pathology; D008228:Lymphoma, Non-Hodgkin / Q000145:classification / Q000235:genetics* / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000235:genetics / Q000473:pathology; D016411:Lymphoma, T-Cell, Peripheral / Q000235:genetics / Q000473:pathology; D016133:Polymerase Chain Reaction / Q000592:standards*; D019310:Pseudolymphoma / Q000235:genetics / Q000473:pathology; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D013624:Taiwan / Q000453:epidemiology