Heat-shock preconditioning protects fatty livers in genetically obese Zucker rats from microvascular perfusion failure after ischemia reperfusion.
Reduced tolerance of steatotic livers to ischemic injury is considered to correlate with impaired microcirculation. The aim of this study was to investigate the impact of heat-shock preconditioning (HSPC) on microcirculatory failure after ischemia/reperfusion (I/R) in steatotic livers by means of intra-vital fluorescence microscopy. Obese Zucker rats were used. In the HS group, rats underwent whole-body hyperthermia followed by 60-min partial liver ischemia. In group IR, rats were exposed only to ischemia. Microcirculation parameters (sinusoidal perfusion rate, sinusoidal diameter, leukocyte-endothelial interaction) were significantly better preserved in the HS group than in the IR group. Liver enzymes, oxygenated glutathione/reduced glutathione (GSSG/GSH) ratio, and electron microscopy showed less damage in the HS group. A marked expression of heat shock protein 72 (HSP72) and heme oxygenase (HO-1) was found only in the livers of group HS. HSPC mitigated the I/R injury of steatotic livers by preventing post-ischemic failure of microcirculation. This beneficial effect was found to be associated with the induction of HSP72 and HO-1.
Yamagami
Kazuhiko
K
Department of Gastroenterological Surgery, Kyoto University Graduate School of Medicine, Kyoto, Japan.
Enders
Georg
G
Schauer
Rolf Josef
RJ
Leiderer
Rosemarie
R
Hutter
Jörg
J
Yamamoto
Yuzo
Y
Yamaoka
Yoshio
Y
Hammer
Claus
C
Messmer
Konrad
K
D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001794:Blood Pressure; D005234:Fatty Liver / Q000473:pathology / Q000503:physiopathology* / Q000601:surgery; D043384:Glutamyl Aminopeptidase / Q000378:metabolism; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000378:metabolism; D018869:Heat-Shock Response; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism; D051547:Heme Oxygenase-1; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000201:enzymology / Q000473:pathology / Q000648:ultrastructure; D008102:Liver Circulation; D016031:Liver Transplantation; D008297:Male; D008833:Microcirculation; D008854:Microscopy, Electron; D051381:Rats; D011924:Rats, Zucker; D015427:Reperfusion Injury / Q000503:physiopathology / Q000517:prevention & control*; D019172:Transplantation Conditioning / Q000379:methods*
D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D007770:L-Lactate Dehydrogenase; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1; D000410:Alanine Transaminase; D043384:Glutamyl Aminopeptidase