Differentiation of follicular dendritic sarcoma cells into functional myeloid-dendritic cell-like elements.
In a rare case of follicluar dendritic cell sarcoma, malignant cells were isolated and cultured in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin 4 (IL-4) and tumor necrosis factor alpha (TNF-alpha). After 14 d, 15% of neoplastic cells differentiated into myeloid-dendritic cell-like elements as demonstrated by immunological and functional characteristics. These cells showed the same cytogenetic abnormality of the malignant clone (fluorescence in situ hybridisation analysis performed on CD1a+ cells) and were able to induce allogenic T-cell proliferation in the mixed leucocyte reaction. These data may indicate that antigen presenting capacity could be a functional state inducible in cellular elements which are believed not to be of hemopoietic origin. Further studies are warranted to confirm these findings and to clarify the possibility to use these cells to generate specific anti-tumoral immune responses.
Della Porta
Matteo
M
Section of Hematology, Department of Biomedical Sciences, University of Ferrara, Italy. sse@dns.unife.it
Rigolin
Gian Matteo
GM
Bugli
Anna Maria
AM
Bardi
Antonella
A
Bragotti
Letizia Zenone
LZ
Bigoni
Renato
R
Cuneo
Antonio
A
Castoldi
Gianluigi
G
D018949:Antigens, CD1 / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D020566:Dendritic Cells, Follicular / Q000187:drug effects / Q000276:immunology / Q000473:pathology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D015847:Interleukin-4 / Q000494:pharmacology; D007951:Leukemia, Myeloid / Q000235:genetics / Q000276:immunology / Q000473:pathology; D008297:Male; D011994:Recombinant Proteins; D012509:Sarcoma / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D018949:Antigens, CD1; C419393:CD1a antigen; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor