Induction of 5alpha-reductase type II mRNA transcription in primary cultured prostate epithelial cells by a soluble factor produced by primary cultured prostate fibroblast cells.
The role of DHT in the development of BPH has resulted in the formulation of several drugs, which have been designed to inhibit the formation of DHT by the 5alpha-reductase enzymes (5alpha-reductase type I (5alpha-RI) & 5alpha-reductase type II (5alpha-RII)). Although the function of these enzymes is well understood, the biochemical stimulus for initiation of 5alpha-RI and II gene expression has not been described. Study of a co-culture model indicated the presence of a diffusible factor secreted by prostatic fibroblast cells, which is responsible for the transcription of 5alpha-II mRNA in primary prostatic epithelial cells. In this study, we describe the partial characterisation of a fibroblast-secreted, soluble factor which we believe induces the transcription of 5alpha-RII mRNA in long-term primary cultures of prostate epithelial cells which can no longer transcribe 5alpha-RII mRNA.
Bayne
C W
CW
Breast Cancer Research Group, Department of Oncology, University of Edinburgh, Paderewski Building, Western General Hospital, Crewe Road South, Edinburgh, EH4 2XU, UK. cbay@srv0.med.ed.ac.uk
Ross
M
M
Inglis
N F
NF
D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase / Q000378:metabolism*; D002478:Cells, Cultured; D004790:Enzyme Induction; D004847:Epithelial Cells / Q000201:enzymology; D005347:Fibroblasts / Q000201:enzymology*; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D011467:Prostate / Q000201:enzymology*; D011470:Prostatic Hyperplasia / Q000201:enzymology; D012333:RNA, Messenger / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D014357:Trypsin / Q000494:pharmacology
D012333:RNA, Messenger; D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase; D014357:Trypsin