Red blood cell antioxidant levels in Wuchereria bancrofti infection.
The elimination of microfilariae of Wuchereria bancrofti is probably mediated by free radicals. Red cell catalase (C), glutathione peroxidase (GPX), and superoxide dismutase (SOD) activity levels were measured as an indirect method of assessing blood oxidant status in 29 asymptomatic microfilaraemics, 29 "endemic normals", and 29 controls living in a non-endemic area. Changes in the activity of these enzymes were also compared over a one month period in 22 asymptomatic microfilaraemics randomised to receive either single dose or 14 day treatment with diethyl carbamazine citrate (DEC). Red cell GPX activity levels were significantly higher in "endemic normals" when compared to mf positive cases and non-endemic controls. An early and significant increase in GPX activity (on days 3, 7 and 14 compared to pretreatment levels, p<0.01) was observed after DEC in both treatment groups. Increases in the activity of catalase and SOD became significant only on days 14 and 30 respectively. The percentage reduction in microfilaraemia correlated significantly with the percentage increase in GPX activity levels (R(2)=0.58, p=0.6 x 10(-5)). Our results may suggest a role for GPX related oxidant species in the elimination of microfilariae.
Premaratna
R
R
Department of Medicine, Faculty of Medicine, University of Kelaniya, P O Box 6, Thalagolla Rd., Ragama, Sri Lanka. ranjan_premaratna@lycos.com
Chandrasena
T G A N
TG
Abeyewickreme
W
W
Chandrasena
L G
LG
Senarath
S
S
de Silva
N R
NR
de Silva
H J
HJ
D000293:Adolescent; D000328:Adult; D017677:Age Distribution; D000368:Aged; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D016022:Case-Control Studies; D002374:Catalase / Q000097:blood; D004049:Diethylcarbamazine / Q000627:therapeutic use*; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D005368:Filariasis / Q000097:blood* / Q000188:drug therapy / Q000453:epidemiology / Q000276:immunology; D005369:Filaricides / Q000627:therapeutic use*; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D008297:Male; D008875:Middle Aged; D017678:Sex Distribution; D013188:Sri Lanka / Q000453:epidemiology; D013482:Superoxide Dismutase / Q000097:blood; D014958:Wuchereria bancrofti / Q000276:immunology / Q000378:metabolism
D000975:Antioxidants; D005369:Filaricides; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D004049:Diethylcarbamazine