Induction of protective immunity in pigs after immunisation with CpG oligodeoxynucleotides formulated in a lipid-based delivery system (Biphasix).
A large number of studies demonstrated the immunostimulatory effects of CpG oligonucleotides (ODN), particularly in mice. In the present study, we evaluated the ability of lipid-based delivery systems to enhance the adjuvant effect of CpG-ODN and protect against infection in a porcine pleuropneumonia model. Increased levels of OmlA-specific antibody were detected in animals immunised with OmlA and CpG-ODN formulated in the delivery system Biphasix-vaccine targeting adjuvant (VTA), compared to pigs immunised with VTA without CpG-ODN or CpG-ODN alone. In addition, the responses induced by VTA/CpG formulation were similar to those induced by the commercial adjuvant VSA; however, VTA formulations caused significantly less tissue damage than VSA.
Alcón
V L
VL
Veterinary Infectious Disease Organization, University of Saskatchewan, Saskatoon, Canada S7N 5E3.
Foldvari
M
M
Snider
M
M
Willson
P
P
Gomis
S
S
Hecker
R
R
Babiuk
L A
LA
Baca-Estrada
M E
ME
D000818:Animals; D001483:Base Sequence; D015226:Dinucleoside Phosphates / Q000276:immunology* / Q000494:pharmacology; D008055:Lipids / Q000276:immunology* / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D009838:Oligodeoxyribonucleotides / Q000276:immunology* / Q000494:pharmacology; D011001:Pleuropneumonia / Q000276:immunology*; D013552:Swine / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology* / Q000494:pharmacology*
D015226:Dinucleoside Phosphates; D008055:Lipids; D009838:Oligodeoxyribonucleotides; D014614:Vaccines, Synthetic; C480625:biphasix-vaccine targeting adjuvant; C015772:CpG dinucleotide