A new IL-1 receptor inhibitor 139A: fermentation, isolation, physico-chemical properties and structure.
Interleukin (IL)-1 is known to be a cytokine which plays a major role in pathological conditions like septic shock, inflammation and auto-immune disease, hence, methods that reduce the activity of IL-1 have an impact on clinical medicine. Inhibiting the binding of IL-1 to IL-1 receptors is one of the methods. A new inhibitor of IL-1 receptor, 139A, was isolated from the fermentation broth of Streptomyces sp.139. It was extracted from the broth filtrate, purified by Diaion HP-20, cation exchange resin and DEAE Sephadex A-25. 139A was identified as polysaccharide, its structure was elucidated on the basis of spectral analysis, the immobilized ligand IL-1 receptor binding assay (IL-ILRBA) proved 139A can competitively inhibits the binding of IL-1 to IL-1 receptors.
Jing
Chen
C
Institute of Medicinal Biotechnology, Chinese Academy of Medica Sciense & Peking Union Medical College, Tiantan, Beijing 100050. PR China. shine0022@sine.com
Jianbo
Wu
W
Yuan
Li
L
Rong
Jiang
J
Baoyi
Li
L
D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005285:Fermentation; D008401:Gas Chromatography-Mass Spectrometry; D015394:Molecular Structure; D008970:Molecular Weight; D009904:Optical Rotation; D011134:Polysaccharides / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D017472:Receptors, Interleukin-1 / Q000037:antagonists & inhibitors*; D012988:Soil Microbiology; D013056:Spectrophotometry, Ultraviolet; D013306:Streptomycetaceae / Q000378:metabolism*
D011134:Polysaccharides; D017472:Receptors, Interleukin-1