title: Generation of dendritic cells from lentiviral vector-transduced CD34+ cells from HIV+ donors.
abstract: Dendritic cells hold promise as adjuvant for immunotherapy for cancer and infectious diseases. We demonstrate that a significant number of cryopreserved peripheral blood CD34(+) cells from HIV-infected subjects can be transduced with a replication-incompetent lentiviral vector expressing HIV antigens. In addition, untransduced and transduced CD34(+) cells from HIV-infected individuals were able to differentiate into dendritic cells with strong T-cell stimulatory capacity. Thus, cryopreserved CD34(+) cells from HIV-infected subjects may prove useful for immunotherapy for HIV/AIDS.
authors.affiliation: Department of Medicine, University of California, San Diego, La Jolla, California, USA. Andreas_Gruber@dfci.harvard.edu
authors.forename: A
authors.initials: A
authors.lastname: Gruber
authors.forename: I
authors.initials: I
authors.lastname: Chen
authors.forename: K L
authors.initials: KL
authors.lastname: Kuhen
authors.forename: J C
authors.initials: JC
authors.lastname: Wheat
authors.forename: P
authors.initials: P
authors.lastname: Law
authors.forename: F
authors.initials: F
authors.lastname: Wong-Staal
mesh_terms: D018952:Antigens, CD34 / Q000378:metabolism*; D002454:Cell Differentiation; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology / Q000821:virology; D005822:Genetic Vectors; D015488:HIV Antigens / Q000235:genetics* / Q000276:immunology; D015658:HIV Infections / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000821:virology; D006801:Humans; D016086:Lentivirus / Q000235:genetics*; D014161:Transduction, Genetic
chemical_list: D018952:Antigens, CD34; D015488:HIV Antigens