Effects of Ca2+ on catalytic activity and conformation of trypsin and alpha-chymotrypsin in aqueous ethanol.
The effects of calcium ions on the conformation and catalytic activity of trypsin and alpha-chymotrypsin were studied in aqueous ethanol. The activity of alpha-chymotrypsin was practically lost within 10 min in the presence of 60% ethanol while trypsin preserved about 40% of its original activity even in 85% ethanol at pH 3. The catalytic activity of alpha-chymotrypsin did not decrease in the presence of 1.2M CaCl2 and 0.6M CaCl2 with trypsin in ethanolic solvent. In the latter case an activation of enzyme was observed. The stabilizing effects of calcium ions were accompanied by an increase in the helical content in both enzymes, as followed by circular dichroism measurements.
Kotormán
M
M
Department of Biochemistry, Faculty of Science, University of Szeged, P.O. Box 533, H-6701, Szeged, Hungary.
Laczkó
I
I
Szabó
A
A
Simon
L M
LM
D002118:Calcium / Q000494:pharmacology*; D002384:Catalysis / Q000187:drug effects; D002918:Chymotrypsin / Q000737:chemistry* / Q000378:metabolism*; D002942:Circular Dichroism; D004795:Enzyme Stability / Q000187:drug effects; D000431:Ethanol / Q000737:chemistry; D011487:Protein Conformation / Q000187:drug effects; D012997:Solvents / Q000737:chemistry; D014357:Trypsin / Q000737:chemistry* / Q000378:metabolism*; D014867:Water / Q000737:chemistry
D012997:Solvents; D014867:Water; D000431:Ethanol; D002918:Chymotrypsin; C117608:alpha-chymotrypsin; D014357:Trypsin; D002118:Calcium