title: Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
abstract: We have accumulated information of the coding sequences of uncharacterized human genes, which are known as KIAA genes, and the number of these genes exceeds 2000 at present. As an extension of this sequencing project, we recently have begun to accumulate mouse KIAA-homologous cDNAs, because it would be useful to prepare a set of human and mouse homologous cDNA pairs for further functional analysis of the KIAA genes. We herein present the entire sequences of 400 mouse KIAA cDNA clones and 4 novel cDNA clones which were incidentally identified during this project. Most of clones entirely sequenced in this study were selected by computer-assisted analysis of terminal sequences of the cDNAs. The average size of the 404 cDNA sequences reached 5.3 kb and that of the deduced amino acid sequences from these cDNAs was 868 amino acid residues. The results of sequence analyses of these clones showed that single mouse KIAA cDNAs bridged two different human KIAA cDNAs in some cases, which indicated that these two human KIAA cDNAs were derived from single genes although they had been supposed to originate from different genes. Furthermore, we successfully mapped all the mouse KIAA cDNAs along the genome using a recently published mouse genome draft sequence.
authors.affiliation: Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Okazaki
authors.forename: Reiko
authors.initials: R
authors.lastname: Kikuno
authors.forename: Reiko
authors.initials: R
authors.lastname: Ohara
authors.forename: Susumu
authors.initials: S
authors.lastname: Inamoto
authors.forename: Hidenori
authors.initials: H
authors.lastname: Aizawa
authors.forename: Shigeki
authors.initials: S
authors.lastname: Yuasa
authors.forename: Daisuke
authors.initials: D
authors.lastname: Nakajima
authors.forename: Takahiro
authors.initials: T
authors.lastname: Nagase
authors.forename: Osamu
authors.initials: O
authors.lastname: Ohara
authors.forename: Hisashi
authors.initials: H
authors.lastname: Koga
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003062:Codon; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data
chemical_list: D003062:Codon; D018076:DNA, Complementary