Regulation of the mesangial cell myofibroblast phenotype by actin polymerization.
Mesangial cells in diverse glomerular diseases become myofibroblast-like, characterized by activation of smooth muscle alpha-actin (alpha-SMA) expression. In cultured mesangial cells, serum-deprivation markedly increases alpha-SMA expression, cell size, and stress fiber formation. Since stress fibers are assembled from actin monomers, we investigated the hypothesis that alterations in stress fiber formation regulate alpha-SMA expression and hypertrophy. Human mesangial cells were treated with agents that disrupt or stabilize actin stress fibers. Depolymerization of actin stress fibers in serum-deprived cells with actin-depolymerizing agents, cytochalasin B (CytB) and latrunculin B (LatB), or with inhibitors of Rho-kinase, Y-27632 and HA-1077 decreased alpha-SMA mRNA as judged by Northern blot analysis. Western blot analysis showed that CytB also reduced alpha-SMA protein levels. In serum-fed cells, agents that stabilized actin stress fibers, jasplakinolide (Jas) and phalloidin, increased alpha-SMA mRNA and protein. Treatment of human or rat mesangial cells with CytB, LatB, or Y-27632 decreased alpha-SMA promoter activity. In contrast, Jas increased promoter activity 5.6-fold in rat mesangial cells. The presence of an RNA polymerase inhibitor blocked degradation of alpha-SMA mRNA in cells treated with CytB suggesting that destabilization of this message is dependent on a newly transcribed or rapidly degraded factor. Inhibition of actin polymerization by CytB, LatB, Y-27623, and HA-1077 inhibited incorporation of (3)[H]-leucine into newly synthesized protein. Additionally, CytB and LatB decreased cell volume as determined by flow cytometry. Collectively, these results indicate that the state of polymerization of the actin cytoskeleton regulates alpha-SMA expression, hypertrophy, and myofibroblast differentiation in mesangial cells.
Patel
Keyur
K
Department of Pathology and Anatomy, Eastern Virginia Medical School, Norfolk 23501, USA.
Harding
Pamela
P
Haney
Lisa B
LB
Glass
William F
WF
D008841:Actin Cytoskeleton / Q000187:drug effects / Q000648:ultrastructure*; D000199:Actins / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D048429:Cell Size / Q000187:drug effects; D002478:Cells, Cultured; D003571:Cytochalasin B / Q000494:pharmacology; D047630:Depsipeptides; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism* / Q000648:ultrastructure; D005920:Glomerular Mesangium / Q000166:cytology*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000378:metabolism; D010456:Peptides, Cyclic / Q000494:pharmacology; D010590:Phalloidine / Q000494:pharmacology; D010641:Phenotype; D011401:Promoter Regions, Genetic; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors; D020871:RNA Stability; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D013844:Thiazoles / Q000494:pharmacology; D053778:Thiazolidines; D015533:Transcriptional Activation; D054460:rho-Associated Kinases
D000199:Actins; D019086:Bridged Bicyclo Compounds, Heterocyclic; D047630:Depsipeptides; D004791:Enzyme Inhibitors; D047908:Intracellular Signaling Peptides and Proteins; D010456:Peptides, Cyclic; D012333:RNA, Messenger; D013844:Thiazoles; D053778:Thiazolidines; C057531:jasplakinolide; D010590:Phalloidine; D003571:Cytochalasin B; D017346:Protein Serine-Threonine Kinases; D054460:rho-Associated Kinases; C037068:latrunculin B