Galactosylated chitosan as a synthetic extracellular matrix for hepatocytes attachment.
Galactose moiety as the hepatocyte anchorage was covalently coupled with chitosan for the development of synthetic extracellular matrix. Hepatocytes adhesion to galactosylated chitosan (GC)-coated polystyrene (PS) dish became as high as 94.7% after 2 h incubation whereas the hepatocytes adhesion to chitosan-coated PS dish was 69.1%, indication of galactose-specific recognition between GC molecules and asialoglycoprotein receptors of hepatocytes. The DNA synthesis of the hepatocytes adhered to GC-coated dish was increased in the presence of epidermal growth factor (EGF) at low concentration of GC (0.05 microg/ml) whereas the DNA synthesis of the hepatocytes adhered to GC-coated dish was decreased in the presence of EGF at high concentration of GC (5 microg/ml). The spreading shapes of the hepatocytes adhered to the surface in the presence of EGF at low concentration of GC (0.05 microg/ml) were enhanced than in the absence of EGF. The hepatocytes adhered to the surface at high concentration of GC (5 microg/ml) showed round shapes and exhibited many spheroid formation after 24 h in the presence of EGF.
Park
In-Kyu
IK
School of Agricultural Biotechnology, Seoul National University, 103 Serdun-dong, Kwonsun-gu, Suwon 441-744, South Korea.
Yang
Jun
J
Jeong
Hwan-Jeong
HJ
Bom
Hee-Seung
HS
Harada
Ichiro
I
Akaike
Toshihiro
T
Kim
Su-Il
SI
Cho
Chong-Su
CS
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D048429:Cell Size / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry*; D048271:Chitosan; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis; D004305:Dose-Response Relationship, Drug; D005109:Extracellular Matrix / Q000737:chemistry / Q000502:physiology*; D005690:Galactose / Q000737:chemistry* / Q000494:pharmacology*; D022781:Hepatocytes / Q000737:chemistry / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*
D020099:Coated Materials, Biocompatible; D002686:Chitin; D048271:Chitosan; D005690:Galactose