Distribution of human group a rotavirus VP7 and VP4 types circulating in Seoul, Korea between 1998 and 2000.
Three hundred forty-eight fecal specimens collected from young children with acute diarrhea in Seoul, Korea between January 1998 and February 2000 were examined for G and P types. Of these, 205 samples (59%) were confirmed as group A rotavirus by ELISA for the detection of VP6 antigen. Confirmed rotavirus isolates were characterized using G serotyping ELISA and RT-PCR methodologies for G and P genotyping of the outer capsid proteins VP7 and VP4, respectively. Serotyping of the outer capsid protein, VP7, revealed G4 as the dominant circulating serotype (41%) followed by G1 (28%) and quite a high incidence of mixed infection (14%). Genotyping of the VP4 protein was carried out on 55 of the rotavirus isolates with the dominant type being P[8] (46%). Of interest were a number of unusual G and P type combinations detected in Korea for the first time, especially the P[4] genotype associated with non-G2 serotypes. There were also a number of P[6] isolates identified including one G2P[6] isolate.
Song
Mi-Ok
MO
Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Korea.
Kim
Ki-Jung
KJ
Chung
Sang-In
SI
Lim
Inseok
I
Kang
Shien-Young
SY
An
Chang-Nam
CN
Kim
Wonyong
W
D000956:Antigens, Viral; D036022:Capsid Proteins / Q000235:genetics*; D002675:Child, Preschool; D003967:Diarrhea / Q000453:epidemiology* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007723:Korea / Q000453:epidemiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification* / Q000235:genetics; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000821:virology; D012703:Serotyping
D000956:Antigens, Viral; D036022:Capsid Proteins; C060339:VP4 protein, Rotavirus; C050779:VP7 protein, Rotavirus