Increased production of bacterial cellulose by Acetobacter sp. V6 in synthetic media under shaking culture conditions.
Acetobacter strains are bacteria that can synthesize cellulose when grown in a complex medium containing glucose. The effect of the components of a synthetic medium on bacterial cellulose (BC) production by a newly isolated Acetobacter sp. V6 in shaking cultures was investigated. BC production was dependent on the presence of MgSO4 x 7H2O and cosubstrates such as ethanol and lactic acid in the medium. The optimal synthetic medium contained 1.5% glucose, 0.2% (NH4)2SO4, 0.3% KH2PO4, 0.3% Na2HPO4 x 12H2O, 0.08% MgSO4 x 7H2O, 0.0005% FeSO4 x 7H2O, 0.0003% H3BO3, 0.00005% nicotinamide, and 0.6% ethanol. A maximum BC concentration of 4.16 g/l was achieved after 8 days of cultivation at 200 rpm. The production of BC by Acetobacter sp. V6 was higher in synthetic medium than complex medium (Hestrin and Schramm medium) traditionally used for Acetobacter strains.
Son
Hong-Joo
HJ
Department of Biotechnology, Miryang National University, Miryang, Kyungnam 627-702, South Korea. shjoo@arang.miryang.ac.kr
Kim
Hee-Goo
HG
Kim
Keun-Ki
KK
Kim
Han-Soo
HS
Kim
Yong-Gyun
YG
Lee
Sang-Joon
SJ
D000091:Acetobacter / Q000201:enzymology*; D001672:Biocompatible Materials; D019149:Bioreactors; D002482:Cellulose / Q000096:biosynthesis*; D003470:Culture Media / Q000737:chemistry
D001672:Biocompatible Materials; D003470:Culture Media; D002482:Cellulose