title: Rapid absorption of luminal polyamines in a rat small intestine ex vivo model.
abstract: OBJECTIVE
  Not only biosynthesis, but also uptake from the intestinal lumen, are important polyamine sources. However, there has been no information regarding dynamic polyamine transport in the small intestine. We evaluated polyamine uptake from the small intestine using a rat ex vivo model.
  
  
  METHODS
  The organ block consisting of the small intestine and blood vessels was used. The isolated small intestine was placed in a warmed saline bath and perfused in a non-circulating manner via the superior mesenteric artery. Radio-labeled putrescine, spermidine or spermine (7.4 x 104 Bq), with 1.0 mL of phosphate buffer saline (pH 7.4) was instilled into the jejunal lumen for 1 min. Blood samples from the portal vein were collected and sample radioactivity was determined. In another experiment, an immunohistochemical study of polyamine was performed.
  
  
  RESULTS
  After 14C-polyamine instillation, radioactivity in the portal vein samples immediately increased and then decreased gradually. The absorptive pattern did not differ among the three polyamines. The recovery rates from radioactivity at the portal vein among the three polyamines were approximately 61-76% during the initial 10 min after the administration of 14C-polyamine, and were not different from each other. Aminoguanidine, which inhibits putrescine degradation, significantly suppressed initial putrescine uptake and recovery percentage. The intraluminal administration of spermine caused an increase in the immunoreactivity of the spermine antibody in the intestinal villi.
  
  
  CONCLUSIONS
  Luminal polyamines were rapidly absorbed by the intestinal mucosa and then subsequently transferred into the portal vein using a rat ex vivo model. The prior administration of aminoguanidine significantly inhibited initial putrescine transport into the portal vein.
authors.affiliation: Division of Gastroenterology, Shiga University of Medical Science, Tsukinowa-cho, Seta, Otsu, Shiga, Japan.
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Uda
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Tsujikawa
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Fujiyama
authors.forename: Tadao
authors.initials: T
authors.lastname: Bamba
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000502:physiology; D007408:Intestinal Absorption / Q000502:physiology*; D007413:Intestinal Mucosa / Q000473:pathology / Q000502:physiology; D007421:Intestine, Small / Q000473:pathology / Q000502:physiology; D008297:Male; D011073:Polyamines / Q000008:administration & dosage / Q000493:pharmacokinetics*; D011700:Putrescine / Q000008:administration & dosage / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D013095:Spermidine / Q000008:administration & dosage / Q000493:pharmacokinetics; D013096:Spermine / Q000008:administration & dosage / Q000493:pharmacokinetics
chemical_list: D011073:Polyamines; D013096:Spermine; D013095:Spermidine; D011700:Putrescine