Regeneration of sciatic nerves of adult rats induced by extracts from distal stumps of pre-degenerated peripheral nerves.
Despite numerous experimental and clinical attempts to reconstruct injuries of peripheral nerves, the methods developed until now have not been sufficiently effective. We examined the influence of extracts (postmicrosomal fractions) obtained from non-pre-degenerated or 7-day-pre-degenerated distal segments of peripheral nerves on the regeneration of injured sciatic nerves of male adult rats. The extracts were introduced to the site of injury with autologous connective tissue chambers filled with fibrin. Reference groups were treated with brain-derived neurotrophic factor (BDNF) or fibrin only. We examined DiI-labeled motoneurons, toluidine blue-labeled myelinated fibers in the mid-part of the chambers, and AChE-positive nerve endings to assess the regeneration intensity. In addition, the length of fibers regrowing within the chambers was measured. We found that extracts obtained from distal stumps of 7-day-pre-degenerated peripheral nerves enhanced nerve regeneration as strongly as BDNF.
Marcol
Wieslaw
W
Department of Physiology, Medical University of Silesia, Katowice, Poland. fzjkatpl@infomed.slam.katowice.pl
Kotulska
Katarzyna
K
Swiech-Sabuda
Ewa
E
Larysz-Brysz
Magdalena
M
Gołka
Beata
B
Górka
Dariusz
D
Lewin-Kowalik
Joanna
J
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology; D005337:Fibrin; D008297:Male; D008854:Microscopy, Electron; D009046:Motor Neurons / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D009410:Nerve Degeneration / Q000503:physiopathology*; D009411:Nerve Endings / Q000187:drug effects / Q000378:metabolism; D009416:Nerve Regeneration / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000187:drug effects / Q000502:physiology / Q000601:surgery / Q000648:ultrastructure; D012828:Silicones; D014020:Tissue Extracts / Q000494:pharmacology*
D019208:Brain-Derived Neurotrophic Factor; D012828:Silicones; D014020:Tissue Extracts; D005337:Fibrin; D000110:Acetylcholinesterase