title: Spatial expression of the alternatively spliced EIIIB and EIIIA segments of fibronectin in the early chicken embryo.
abstract: Using domain-specific antibodies, we have analyzed the tissue distribution of fibronectins (FNs) containing the alternatively spliced EIIIB and EIIIA segments relative to total FN in early chicken embryos. The results show a selective loss of EIIIA+ FN staining in the notochordal sheath and in cartilaginous structures between 4.5 and 7.0 days of development. In other regions, EIIIB+ and EIIIA+ FNs are extensively codistributed in and around mesoderm-derived structures (somites, notochord, heart, and blood vessels), in basal laminae of endoderm and ectoderm-derived structures, as well as within the vicinity of neural crest formation and migration. We also noted that EIIIA staining overlaps with spatial patterns of distribution that have previously been described for the alpha4 integrin subunit, a component of the EIIIA receptor alpha4beta1.
authors.affiliation: Sacramento VA Medical Center, VA Northern California Health Care System, Mather, CA 95655, USA. John.Peters3@med.va.gov
authors.forename: John
authors.initials: J
authors.lastname: Peters
authors.forename: Jack
authors.initials: J
authors.lastname: Sechrist
authors.forename: Simone
authors.initials: S
authors.lastname: Luetolf
authors.forename: Grace
authors.initials: G
authors.lastname: Loredo
authors.forename: Marianne
authors.initials: M
authors.lastname: Bronner-Fraser
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D002319:Cardiovascular System / Q000196:embryology / Q000378:metabolism; D002356:Cartilage / Q000196:embryology / Q000378:metabolism; D002642:Chick Embryo; D004064:Digestive System / Q000196:embryology / Q000378:metabolism; D005314:Embryonic and Fetal Development; D004702:Endocrine Glands / Q000196:embryology / Q000378:metabolism; D005353:Fibronectins / Q000235:genetics / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D005855:Germ Layers / Q000378:metabolism*; D039441:Integrin alpha4 / Q000235:genetics / Q000378:metabolism*; D008168:Lung / Q000196:embryology / Q000378:metabolism; D009432:Neural Crest / Q000378:metabolism*; D009928:Organ Specificity; D014018:Tissue Distribution; D014566:Urogenital System / Q000196:embryology / Q000378:metabolism
chemical_list: D005353:Fibronectins; D039441:Integrin alpha4