Coupling capillary electrochromatography with electrospray Fourier transform mass spectrometry for characterizing complex oligosaccharide pools.
To deal with the complexity of the glycan mixtures released from glycoproteins, an efficient form of micro-column separations, capillary electrochromatography, has been combined with high-performance mass spectrometry (Fourier transform ion cyclotron resonance). Contour plots have been generated from the mixtures of O-linked oligosaccharides originated from bovine mucin and bile salt-stimulated lipase, a large glycoprotein enzyme.
Que
Amy H
AH
Department of Chemistry, Indiana University, Bloomington, Indiana 47405, USA.
Mechref
Yehia
Y
Huang
Yunping
Y
Taraszka
John A
JA
Clemmer
David E
DE
Novotny
Milos V
MV
D000818:Animals; D002417:Cattle; D002845:Chromatography / Q000295:instrumentation / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D005583:Fourier Analysis; D006023:Glycoproteins / Q000737:chemistry*; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D009077:Mucins / Q000737:chemistry; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry*; D002787:Sterol Esterase / Q000737:chemistry
D006023:Glycoproteins; D009077:Mucins; D009844:Oligosaccharides; C054784:bile salt-stimulated lipase; D002787:Sterol Esterase