First in vitro directed biosynthesis of new compounds by a minimal type II polyketide synthase: evidence for the mechanism of chain length determination.
The minimal actinorhodin polyketide synthase bearing two point mutations (KSbeta Q161A, ACP C17S) was chemically modified to carry novel C4 to C8 starter units on the ACP: on incubation with an excess of malonyl CoA new 16-carbon polyketides are made, supporting a measuring mechanism.
Nicholson
T P
TP
School of Chemistry, University of Bristol, Cantock's Close, Bristol, UK BS8 1TS.
Winfield
C
C
Westcott
J
J
Crosby
J
J
Simpson
T J
TJ
Cox
R J
RJ
D000213:Acyl Carrier Protein / Q000378:metabolism; D000217:Acyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000880:Anthraquinones / Q000737:chemistry / Q000378:metabolism*; D001426:Bacterial Proteins; D008316:Malonyl Coenzyme A / Q000378:metabolism; D048630:Polyketide Synthases; D021241:Spectrometry, Mass, Electrospray Ionization; D013302:Streptomyces / Q000201:enzymology; D000095622:Benzoisochromanequinones
D000213:Acyl Carrier Protein; D000217:Acyltransferases; D000880:Anthraquinones; D001426:Bacterial Proteins; D008316:Malonyl Coenzyme A; D048630:Polyketide Synthases; C087193:polyketide beta-ketoacylsynthase; C013390:actinorhodin; D000095622:Benzoisochromanequinones