Analysis of gene expression in the developing mouse retina.
In the visual system, differential gene expression underlies development of the anterior-posterior and dorsal-ventral axes. Here we present the results of a microarray screen to identify genes differentially expressed in the developing retina. We assayed gene expression in nasal (anterior), temporal (posterior), dorsal, and ventral embryonic mouse retina. We used a statistical method to estimate gene expression between different retina regions. Genes were clustered according to their expression pattern and were ranked within each cluster. We identified groups of genes expressed in gradients or with restricted patterns of expression as verified by in situ hybridization. A common theme for the identified genes is the differential expression in the dorsal-ventral axis. By analyzing gene expression patterns, we provide insight into the molecular organization of the developing retina.
Díaz
Elva
E
Department of Molecular and Cell Biology, Functional Genomics Laboratory, Helen Wills Neuroscience Institute, University of California, Berkeley, CA 94720, USA.
Yang
Yee Hwa
YH
Ferreira
Todd
T
Loh
Kenneth C
KC
Okazaki
Yasushi
Y
Hayashizaki
Yoshihide
Y
Tessier-Lavigne
Marc
M
Speed
Terence P
TP
Ngai
John
J
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D020869:Gene Expression Profiling; D051379:Mice; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D012160:Retina / Q000201:enzymology / Q000378:metabolism*
D017931:DNA Primers