title: Expression of TNF-receptor-associated factor genes in murine tooth development.
abstract: Tumor necrosis factor receptor-associated factors (TRAFs) belong to a family of intracellular adaptor proteins that mediate signaling downstream of various cell surface receptors. We carried out comparative in situ hybridization analysis of five Traf genes Traf1, Traf2, Traf3, Traf4 and Traf6 during murine odontogenesis from the formation of the epithelial thickening to the early bell stage. Traf2, Traf3 and Traf6 showed weak expression in the thickened epithelium. Expression of Traf1, Traf2 and Traf6 were observed in the outer edges of the bud epithelium whereas Traf3 was strongly expressed at the tip of the bud epithelium. Expression of Traf1, Traf4 and Traf6 were detected in the dental papilla mesenchyme. Traf2 showed restricted expression in the internal enamel epithelium of the bell stage while expression of Traf1, Traf3, Traf4 and Traf6 were observed in both the internal and the external enamel epithelium. During early odontogenesis, all five genes show dynamic spatiotemporal expression patterns.
authors.affiliation: Department of Craniofacial Development, GKT Dental Institute, King's College London, Guy's Hospital, Floor 28, Guy's Tower, London Bridge, London SE1 9RT, UK.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ohazama
authors.forename: Jo-Maree
authors.initials: JM
authors.lastname: Courtney
authors.forename: Paul T
authors.initials: PT
authors.lastname: Sharpe
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D051379:Mice; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D047990:TNF Receptor-Associated Factor 1; D047992:TNF Receptor-Associated Factor 2; D048008:TNF Receptor-Associated Factor 3; D053449:TNF Receptor-Associated Factor 4; D048029:TNF Receptor-Associated Factor 6; D014070:Tooth / Q000196:embryology*; D047988:Tumor Necrosis Factor Receptor-Associated Peptides and Proteins
chemical_list: D011506:Proteins; D047990:TNF Receptor-Associated Factor 1; D047992:TNF Receptor-Associated Factor 2; D048008:TNF Receptor-Associated Factor 3; D053449:TNF Receptor-Associated Factor 4; D048029:TNF Receptor-Associated Factor 6; C506368:Traf4 protein, mouse; D047988:Tumor Necrosis Factor Receptor-Associated Peptides and Proteins