title: Cutting edge: conventional CD8 alpha+ dendritic cells are preferentially involved in CTL priming after footpad infection with herpes simplex virus-1.
abstract: CTL play a major role in immunity to HSV type 1, but little is known about the priming process. In this study, we have examined the class I-restricted presentation of an immunodominant determinant from HSV-1 glycoprotein B after footpad infection. We have found that the only cell types capable of presenting this determinant in draining popliteal lymph nodes within the first 3 days after infection are the CD11c(+)CD8alpha(+)CD45RA(-) dendritic cells. Given that such class I-restricted presentation is essential for CTL priming, this implies that these conventional CD8alpha(+) dendritic cells are the key subset involved in CTL immunity to this virus.
authors.affiliation: Cooperative Research Center for Vaccine Technology, University of Melbourne, Victoria, Australia.
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Smith
authors.forename: Gabrielle T
authors.initials: GT
authors.lastname: Belz
authors.forename: Nicholas S
authors.initials: NS
authors.lastname: Wilson
authors.forename: Jose A
authors.initials: JA
authors.lastname: Villadangos
authors.forename: Ken
authors.initials: K
authors.lastname: Shortman
authors.forename: Francis R
authors.initials: FR
authors.lastname: Carbone
authors.forename: William R
authors.initials: WR
authors.lastname: Heath
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D000938:Antigen-Presenting Cells / Q000276:immunology / Q000378:metabolism / Q000821:virology; D016827:CD8 Antigens / Q000096:biosynthesis*; D003602:Cytotoxicity, Immunologic / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism* / Q000821:virology; D018984:Epitopes, T-Lymphocyte / Q000276:immunology / Q000378:metabolism; D006561:Herpes Simplex / Q000276:immunology* / Q000821:virology; D018259:Herpesvirus 1, Human / Q000276:immunology*; D006614:Hindlimb; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D006825:Hybridomas; D007110:Immunity, Active; D016056:Immunodominant Epitopes / Q000276:immunology / Q000378:metabolism; D007279:Injections, Subcutaneous; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology / Q000378:metabolism
chemical_list: D016827:CD8 Antigens; C431698:CD8alpha antigen; D018984:Epitopes, T-Lymphocyte; D015395:Histocompatibility Antigens Class I; D016056:Immunodominant Epitopes; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus