title: Investigating the possibility of monitoring lectin levels in commercial soybean meals intended for poultry feeding using steam-heated soybean meal as a model.
abstract: Native soybean lectins (SBL) could potentially have deleterious effects on young animals. The objectives of this study were to determine the optimum processing temperature and time at which SBL is inactivated and to investigate the possibility of using urease activity (UA) to predict residual lectin levels in soybean meal (SBM). Raw defatted SBM was steam-heated at incremental temperatures between 90 and 120 degrees C for 5 to 20 min in an autoclave. The processed meals were subjected to native-PAGE and measurement of total carbohydrate-binding lectin (TCBL), agglutinating lectin (AL), UA, and trypsin inhibitor (TI). Processing severity was evaluated by determining protein solubility in 0.2% potassium hydroxide. Results indicated that levels of all antinutrients (TCBL, AL, UA, and TI) decreased with increasing processing temperature (P < 0.05). The intensity of the lectin band on the electrophoresis gel was considerably reduced when meal was heated at 100 degrees C for 5 min. This result implied that lectin inactivation occurred at 100 degrees C. More than 90% of all the original antinutrient levels in the raw meal were destroyed when meals were heated at 100 degrees C for 5 min. Meals processed at 100 degrees C for 5 to 20 min had protein solubility values (80 to 85%) indicative of adequate processing. The denaturation pattern of UA was highly correlated with that of SBL (r > or = 0.73), indicating that UA could be used for monitoring lectin levels in commercial meals. We concluded that UA of 0.03 to 0.09 units of pH change are indicative of adequately processed meals that contain negligible lectin levels.
authors.affiliation: Department of Animal and Poultry Science, University of Saskatchewan, Saskatoon S7N 5A8, Saskatchewan, Canada. fasinyo@acesag.auburn.edu
authors.forename: Y O
authors.initials: YO
authors.lastname: Fasina
authors.forename: H L
authors.initials: HL
authors.lastname: Classen
authors.forename: J D
authors.initials: JD
authors.lastname: Garlich
authors.forename: H E
authors.initials: HE
authors.lastname: Swaisgood
authors.forename: D A
authors.initials: DA
authors.lastname: Clare
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D005511:Food Handling / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D009753:Nutritive Value; D037121:Plant Lectins / Q000032:analysis* / Q000378:metabolism; D011200:Poultry / Q000378:metabolism*; D030262:Soybean Proteins / Q000032:analysis* / Q000378:metabolism; D013696:Temperature; D013997:Time Factors; D014361:Trypsin Inhibitors / Q000032:analysis; D014510:Urease / Q000378:metabolism; D010835:Phytohemagglutinins
chemical_list: D037121:Plant Lectins; D030262:Soybean Proteins; D014361:Trypsin Inhibitors; D014510:Urease; C028824:soybean lectin; D010835:Phytohemagglutinins