title: The isoprenoid biosynthetic pathway in Saccharomyces cerevisiae is affected in a maf1-1 mutant with altered tRNA synthesis.
abstract: tRNA isopentenylation is a branch of an isoprenoid pathway in yeast. There is a competition for a substrate between isoprenoid biosynthetic enzyme Erg20p and tRNA isopentenyltransferase. Here we studied the direct effect of elevated tRNA biosynthesis on ERG20 expression. The maf1-1 mutant of Saccharomyces cerevisiae that has enhanced cellular tRNA levels was used. We show that both ERG20 transcript and Erg20 protein levels are increased in maf1-1. Additionally, maf1-1 leads to decreased ergosterol content in the cells. These effects of maf1-1 are dependent on functional tRNA isopentenyltransferase. Our results indicate that a complex regulation of the isoprenoid pathway involves also an effect of changes in tRNA biosynthesis.
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Kamińska
authors.forename: Kariona
authors.initials: K
authors.lastname: Grabińska
authors.forename: Marta
authors.initials: M
authors.lastname: Kwapisz
authors.forename: Jacek
authors.initials: J
authors.lastname: Sikora
authors.forename: Wieslaw J
authors.initials: WJ
authors.lastname: Smagowicz
authors.forename: Grazyna
authors.initials: G
authors.lastname: Palamarczyk
authors.forename: Teresa
authors.initials: T
authors.lastname: Zoładek
authors.forename: Magdalena
authors.initials: M
authors.lastname: Boguta
mesh_terms: D019883:Alkyl and Aryl Transferases; D015152:Blotting, Northern; D004875:Ergosterol / Q000096:biosynthesis / Q000235:genetics; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D009154:Mutation; D011106:Polyisoprenyl Phosphates / Q000096:biosynthesis*; D012343:RNA, Transfer / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D005656:Fungal Proteins; C104954:MAF1 protein, S cerevisiae; D011106:Polyisoprenyl Phosphates; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D012343:RNA, Transfer; D019883:Alkyl and Aryl Transferases; C068392:MOD5 protein, S cerevisiae; D004875:Ergosterol