Vitamin E modulates radiation-induced oxidative damage in mice fed a high-lipid diet.
The Vitamin E (VE) effect was examined on oxidative damage to DNA, lipids, and protein in mice that were fed various levels of lipid diets after total body irradiation (TBI) with X-rays at 2 Gy. No increase of 8-hydroxydeoxyguanosine (8OHdG) by TBI was observed in the + VE group; however, in the case of the -VE group, a significantly higher 8OHdG level was observed in the high-lipid group than in the low- or basal-lipid group. In the groups with TBI, the concentration of thiobarbituric reactive substances (TBARS) only significantly increased in the high-lipid (-VE) group. These changes in TBARS, due to TBI, were not detected in other groups. The contents of protein carbonyls only increased in the (-VE) group. The contents of protein carbonyls was significantly different between the (+VE) and the (-VE) groups, regardless of the lipid levels. The concentrations of GSH, vitamins C and E in the liver were lower, and the concentration of non-heme iron in the liver was higher in the high-lipid group than in the low- and basal-lipid groups. These concentrations in the high-lipid group were significantly different between the (+VE) and the (-VE) groups. These results strongly suggest that mice that are fed a high-lipid diet are susceptible to TBI-induced oxidative damage. Also, decreases in the GSH levels and an increase in the iron level are involved in the mechanism of this susceptibility.
Shin
Sung-Jae
SJ
Department of Food Science Research for Health, National Institute of Health and Nutrition, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8636, Japan. mhjrm@nih.go.jp
D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000032:analysis; D001835:Body Weight; D004032:Diet; D005978:Glutathione / Q000032:analysis; D008055:Lipids / Q000008:administration & dosage*; D008099:Liver / Q000737:chemistry / Q000187:drug effects / Q000528:radiation effects; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D019159:Nonheme Iron Proteins / Q000032:analysis; D010084:Oxidation-Reduction / Q000528:radiation effects; D018384:Oxidative Stress / Q000187:drug effects*; D014810:Vitamin E / Q000494:pharmacology*; D014916:Whole-Body Irradiation
D000975:Antioxidants; D008055:Lipids; D019159:Nonheme Iron Proteins; D014810:Vitamin E; D005978:Glutathione; D001205:Ascorbic Acid