Aspergillus oryzae in solid-state and submerged fermentations. Progress report on a multi-disciplinary project.
We report the progress of a multi-disciplinary research project on solid-state fermentation (SSF) of the filamentous fungus Aspergillus oryzae. The molecular and physiological aspects of the fungus in submerged fermentation (SmF) and SSF are compared and we observe a number of differences correlated with the different growth conditions. First, the aerial hyphae which occur only in SSFs are mainly responsible for oxygen uptake. Second, SSF is characterised by gradients in temperature, water activity and nutrient concentration, and inside the hyphae different polyols are accumulating. Third, pelleted growth in SmF and mycelial growth in SSF show different gene expression and protein secretion patterns. With this approach we aim to expand our knowledge of mechanisms of fungal growth on solid substrates and to exploit the biotechnological applications.
te Biesebeke
Rob
R
Wageningen Centre for Food Sciences, P.O. Box 557, The Netherlands.
Ruijter
George
G
Rahardjo
Yovita S P
YS
Hoogschagen
Marisca J
MJ
Heerikhuisen
Margreet
M
Levin
Ana
A
van Driel
Kenneth G A
KG
Schutyser
Maarten A I
MA
Dijksterhuis
Jan
J
Zhu
Yang
Y
Weber
Frans J
FJ
de Vos
Willem M
WM
van den Hondel
Kees A M J J
KA
Rinzema
Arjen
A
Punt
Peter J
PJ
D001236:Aspergillus oryzae / Q000235:genetics / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods; D005285:Fermentation / Q000502:physiology; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal / Q000502:physiology; D025301:Hyphae / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption
D010100:Oxygen