Sequence and phylogenetic analysis of the fusion protein cleavage site of Newcastle disease virus field isolates from Iran.
Nine Newcastle disease virus (NDV) isolates from Newcastle disease (ND) outbreaks in different regions of Iran were characterized at molecular level. Sequence analysis revealed that the isolates shared two pairs of arginine and a phenylalanine at the N-terminus of the fusion (F) protein cleavage site similarly to other velogenic isolates of NDV characterized earlier. Eight of the nine isolates had the same amino acid sequence as VOL95, a Russian NDV isolate from 1995. However, one isolate, MK13 showed 5 amino acid substitutions, of which 3 have been reported for other velogenic NDV isolates. These results suggest that the origin of the outbreaks of ND in different parts of Iran in 1995-1998 is VOL95.
Kianizadeh
M
M
Faculty of Veterinary Medicine, University Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
Aini
I
I
Omar
A R
AR
Yusoff
K
K
Sahrabadi
M
M
Kargar
R
R
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004196:Disease Outbreaks / Q000662:veterinary*; D007492:Iran; D008969:Molecular Sequence Data; D009521:Newcastle Disease / Q000453:epidemiology* / Q000821:virology*; D009522:Newcastle disease virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D011200:Poultry; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014760:Viral Fusion Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D014760:Viral Fusion Proteins