title: Quantification of polycyclic aromatic hydrocarbons (PAHs) in human hair by HPLC with fluorescence detection: a biological monitoring method to evaluate the exposure to PAHs.
abstract: A high-performance liquid chromatographic (HPLC) method with fluorescence detection was developed for the quantification of polycyclic aromatic hydrocarbons (PAHs) in human hair. Fifteen kinds of PAHs classified as priority pollutants by the US EPA were quantified with four perdeuterated PAHs as internal standards. After 50 mg hair samples were washed with n-hexane to remove external contamination of PAHs, the samples were digested in 2.5 M sodium hydroxide. The digests were extracted with n-hexane and then analyzed by HPLC. Eleven kinds of PAHs were identified in hair samples of 20 subjects, and 10 kinds of PAHs were eventually quantified using the internal standards. For anthracene, chrysene and benzo[k]fluoranthene, significant differences were observed between smokers and non-smokers. Although benzo[b]fluoranthene, dibenz[a,h]anthracene, benzo[ghi]perylene and indeno[1,2,3-cd]pyrene were observed in the particulates of indoor and outdoor air, they were not detected in all hair samples. The analysis of PAHs in human hair should be useful as a new biomarker to evaluate the exposure to PAHs.
authors.affiliation: Graduate School of Natural Science and Technology, Kanazawa University, 13-1, Takara-machi, Kanazawa, Ishikawa 920-0934, Japan. toriba@p.kanazawa-u.ac.jp
authors.forename: Akira
authors.initials: A
authors.lastname: Toriba
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Kuramae
authors.forename: Thaneeya
authors.initials: T
authors.lastname: Chetiyanukornkul
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Kizu
authors.forename: Tsunehisa
authors.initials: T
authors.lastname: Makino
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakazawa
authors.forename: Kazuichi
authors.initials: K
authors.lastname: Hayakawa
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005069:Evaluation Studies as Topic; D006197:Hair / Q000737:chemistry*; D006801:Humans; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons