Immunosensor for detection of Legionella pneumophila using surface plasmon resonance.
Immunosensor using surface plasmon resonance (SPR) onto self-assembled protein G layer was developed for the detection of Legionella pneumophila. A self-assembled protein G layer on gold (Au) surface was fabricated by adsorbing a mixture of 11-mercaptoundecanoic acid (MUA) and hexanethiol (molar ratio of 1:2) and the activation process for chemical binding between free amine (-NH(2)) of protein G and 11-(MUA) using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDAC) in series. The formation of self-assembled protein G layer on Au substrate and the binding of antibody and antigen in series were confirmed by SPR spectroscopy. The surface morphology analyses of self-assembled protein G layer on Au substrate and monoclonal antibody against L. pneumophila immobilized on protein G were performed by atomic force microscope (AFM). The immunosensor for detection of L. pneumophila using SPR was developed and its detection limit could find up to 10(5) cells/ml.
Oh
Byung-Keun
BK
Department of Chemical Engineering, Sogang University, C.P.O. Box 1142, 100-611, Seoul, South Korea.
Kim
Young-Kee
YK
Lee
Woochang
W
Bae
Young-Min
YM
Lee
Won-Hong
WH
Choi
Jeong-Woo
JW
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods*; D016952:Legionella pneumophila / Q000276:immunology / Q000302:isolation & purification*; D018625:Microscopy, Atomic Force; D009419:Nerve Tissue Proteins / Q000276:immunology; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods*; D013499:Surface Properties
D020099:Coated Materials, Biocompatible; C029152:G-substrate; D009419:Nerve Tissue Proteins