title: The impact of StuI digestion in situ on FISH to human chromosomes with satellite DNA probes.
abstract: Human metaphase chromosomes were digested with StuI and subsequently hybridized in situ using chromosome 9 alphoid DNA and classical satellite III DNA as probes. The data obtained suggest that it is not possible to establish a general rule regarding the cytological effects induced by restriction enzymes in particular chromosome regions and that a number of factors, such as DNA sequences, DNA-protein interaction and enzyme structure, play a role in determining such effects.
authors.affiliation: Dipartimento di Scienze Applicate ai Biosistemi, Sezione di Biologia e Genetica, Cittadella Universitaria, 09042 Monserrato, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Nieddu
authors.forename: G
authors.initials: G
authors.lastname: Pichiri
authors.forename: V
authors.initials: V
authors.lastname: Melis
authors.forename: R
authors.initials: R
authors.lastname: Mezzanotte
mesh_terms: D002899:Chromosomes, Human, Pair 9 / Q000235:genetics*; D015342:DNA Probes / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000235:genetics*; D004276:DNA, Satellite / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence
chemical_list: D015342:DNA Probes; D004276:DNA, Satellite; D004262:DNA Restriction Enzymes