Microdevice with integrated dialysis probe and biosensor array for continuous multi-analyte monitoring.
The simultaneous on-line determination of glucose and lactate using a microdevice that consisted of a dialysis sampling system incorporated to the flow-through cell of a microfabricated biosensor array is presented. The fluidic connections between the different device's components were realized by subsequent processing of stacked dry resist layers on a plastic support that provided also the means for electric connections. The performance of the device was evaluated in vitro. The cross-talk effect on the downstream sensor was investigated and found to be negligible. Recoveries of over 95% for both analytes were achieved when flow rates of the perfusion fluid </=0.5 microl/min were used. At this flow rate, the response time of the device was 2.4 min, which is acceptable for on-line analysis. The linear response concentration range extended up to 30 mM for glucose and 15 mM for lactate. Interference from electroactive species such as ascorbic acid, 2-acetamidophenol and uric acid, was minimal (less than 5% increase in biosensors signal for all substances tested). In addition, the device presented long-term run stability both in buffer and serum samples.
Petrou
P S
PS
Immunoassay Laboratory, I.R.-R.P., N.C.S.R Demokritos, 15310, Athens, Greece.
Moser
I
I
Jobst
G
G
D016477:Artifacts; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005947:Glucose / Q000032:analysis*; D019344:Lactic Acid / Q000032:analysis*; D017551:Microdialysis / Q000295:instrumentation* / Q000379:methods; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods; D008904:Miniaturization; D012680:Sensitivity and Specificity; D018511:Systems Integration
D019344:Lactic Acid; D005947:Glucose