Transcoronary sinus delivery of autologous bone marrow and angiogenesis in pig models with myocardial injury.
OBJECTIVE
The aim of the investigation is to study myocardial injury on pig model with two objectives: (1) feasibility of stimulating angiogenesis with fresh autologous bone marrow; (2) administration of the same fresh autologous bone marrow via coronary sinus with transitory occlusion.


METHODS
A controlled study was done in animal model with three phases, in a study group of 12 pigs (bone marrow administration) as well as in control group of 4 pigs (saline administration). Phase 1-production of coronary stenosis and myocardial injury; Phase 2-two weeks later, administration of bone marrow through coronary sinus with 10 min occlusion in the study group and saline solution in the control group. Phase 3-two weeks later, histological staining with hematoxylin-eosin and inmunohistochemical staining with monoclonal antibody for smooth muscle alpha-actin were conducted on both study and control groups.


RESULTS
The percentage of angionenesis observed in the study group was 91% and 0% in control group. Counting of positive actin in affected and control areas showed statistically significant differences in relation to both groups: study group (1.37 vs. 0.79) and control group (0.47 vs. 0.51). The percentage of mononuclear immature cells observed in the myocardium in the study group was 25% and in the control group was 0%. There was no increment in the coronary collateral circulation when comparing coronary angiography.


CONCLUSIONS
Autologous bone marrow in animal model with experimental myocardial injury enhances angiogenesis, as well as vessels with smooth muscles. The transitory occlusion of the coronary sinus might be an effective way to administer cells as those from the bone marrow.
Vicario
J
J
J.B. Iturraspe Hospital, 4299 av. 7 Jefes, 3000, Santa Fe, Argentina. josevicaro@hotmail.com
Piva
J
J
Pierini
A
A
Ortega
H H
HH
Canal
A
A
Gerardo
L
L
Pfeiffer
H
H
Campos
C
C
Fendrich
I
I
Novero
R
R
Monti
A
A
D000818:Animals; D016026:Bone Marrow Transplantation; D006328:Cardiac Catheterization; D003326:Coronary Circulation / Q000187:drug effects / Q000502:physiology; D023921:Coronary Stenosis / Q000150:complications* / Q000503:physiopathology / Q000628:therapy*; D004195:Disease Models, Animal; D005240:Feasibility Studies; D006335:Heart Injuries / Q000209:etiology* / Q000503:physiopathology / Q000628:therapy*; D018919:Neovascularization, Physiologic / Q000187:drug effects* / Q000502:physiology; D011897:Random Allocation; D012850:Sinus of Valsalva / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology; D013552:Swine; D013997:Time Factors; D014182:Transplantation, Autologous