HNK-1-Reactive oligosaccharide, sulfate-O-3GlcAbeta1-4Xylbeta1-MU, synthesized by cultured human colorectal cancer cells.
Human colorectal cancer cells were incubated with medium containing 4-methylumbelliferyl-beta-D-xyloside (Xyl-MU). The cells synthesized Xyl-MU-derivatives which were detected in the culture medium by gel-filtration high-performance liquid chromatography. These included a Xyl-MU-induced glycosaminoglycan and its biosynthetic intermediates, Galbeta1-4Xylbeta1-MU and Galbeta1-3Galbeta1-4Xylbeta1-MU, and other Xyl-MU-induced oligosaccharides, not related to Xyl-MU-induced glycosaminoglycan, were also synthesized. One of these oligosaccharides, sulfate-O-3GlcAbeta1-4Xylbeta1-MU, reacted with HNK-1, a mouse monoclonal antibody raised against human natural killer cells. Human neural cells and skin fibroblasts have also been reported to synthesize HNK-1-reactive sugar chains. Since HNK-1-reactive sugar chains are known to be involved in cell adhesion in the nervous system, the present results suggest that epithelium-derived colorectal cancer cells might also be able to utilize them in cell adhesion.
Shibata
Shigeru
S
Department of Biochemistry, Hirosaki University School of Medicine, Hirosaki 036-8562, Japan. sshibata@cc.hirosaki-u.ac.jp
Takagaki
Keiichi
K
Ishido
Keinosuke
K
Konn
Mitsuru
M
Sasaki
Mutsuo
M
Endo
Masahiko
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000187:drug effects*; D018959:CD57 Antigens / Q000187:drug effects*; D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D015179:Colorectal Neoplasms / Q000378:metabolism*; D003470:Culture Media; D004187:Disaccharides / Q000737:chemistry*; D005966:Glucuronidase / Q000737:chemistry; D006025:Glycosaminoglycans / Q000737:chemistry; D006801:Humans; D006868:Hydrolysis; D006923:Hymecromone / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000494:pharmacology; D051379:Mice; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
C479127:3-glucuronosyl-1-4-(xylosyl-1-)-4-methylumbelliferone sulfate; D000911:Antibodies, Monoclonal; D018959:CD57 Antigens; D003470:Culture Media; D004187:Disaccharides; D006025:Glycosaminoglycans; D009844:Oligosaccharides; D006923:Hymecromone; C014111:methylumbelliferyl-beta-D-xyloside; D005966:Glucuronidase