Identification of a specifically expressed modified form of novel PSP-94 protein in the secretion of benign prostatic hyperplasia.
Using two-dimensional gel electrophoresis, we investigated the profile of prostatic secretory proteins in human expressed prostatic secretion (EPS) from benign prostate hyperplasia (BPH) patients and compared the patterns with normal controls. We identified three specifically expressed proteins, including prostate secretory protein 61 (PSP61), in EPS from benign BPH patients but absent in normal controls. In addition, we found that PSP61 was a modified isoform of the well-documented PSP-94, which had a perfect matching (100% homology) to the first 61 amino acids of the PSP-94 protein but with a deleted C-terminus. This shortened PSP61 was not due to alternative splicing of the PSP-94 gene at transcription level. Our results provide first evidence on the possibility of using PSP61 as a specific biological marker for diagnosis of BPH.
Xu
Kexin
K
Cancer Biology Lab, Department of Anatomy, Laboratory Block, Faculty of Medicine, The University of Hong Kong, HKSAR, China.
Wang
Xianghong
X
Ling
Ming-Tat
MT
Lee
Davy T W
DT
Fan
Tianyong
T
Chan
Franky L
FL
Xuan
James J W
JJ
Tsao
Sai-Wah
SW
Wong
Yong-Chuan
YC
D000368:Aged; D017398:Alternative Splicing; D016022:Case-Control Studies; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D011470:Prostatic Hyperplasia / Q000378:metabolism*; D029584:Prostatic Secretory Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D020033:Protein Isoforms; D011499:Protein Processing, Post-Translational; D020133:Reverse Transcriptase Polymerase Chain Reaction
D009363:Neoplasm Proteins; D029584:Prostatic Secretory Proteins; D020033:Protein Isoforms; C043076:beta-microseminoprotein