title: [Isolation, purification, and properties of factor XII and its active fragment (beta-XIIa)].
abstract: A procedure of isolation of human blood plasma prekallikrein, coagulation factor XII and active fragment beta-XIIa has been developed. This procedure includes the traditional chromatography steps and FPLC. Disc-electrophoresis revealed that the preparations of factor XII and beta-XIIa were homogeneous. Their specific activity was 70.6 U and 2.5 U, respectively. The procedure described is less time consuming and it allows to isolate these factors in the preparative quantities.
authors.affiliation: Russian Medical Academy of Postgraduate Education, Barrikadnaya st., 2, Moscow, 123995 Russia.
authors.forename: T B
authors.initials: TB
authors.lastname: Blokhina
authors.forename: E A
authors.initials: EA
authors.lastname: Neshkova
authors.forename: G A
authors.initials: GA
authors.lastname: Iarovaia
mesh_terms: D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005174:Factor XII / Q000737:chemistry* / Q000302:isolation & purification*; D006801:Humans
chemical_list: D005174:Factor XII
vernacular_title: Poluchenie, ochistka, svoĭstva faktora XII i ego aktivnogo fragmenta (beta-XIIa).