Magnetic resonance image attributes of the bovine corpus luteum during development and regression.
To determine whether magnetic resonance (MR) image attributes of the corpus luteum (CL) reflect its physiologic status at different phases of the bovine ovarian cycle, we analyzed the numerical pixel values (NPVs), relaxation rates, proton densities (PDs), and apparent diffusion coefficients (ADCs) from T(1)-, T(2)-, and diffusion-weighted in vitro images and maps of the CL acquired at defined phases of luteal function. Ovaries were removed and serum samples taken on days 3, 6, and 10, or >/=17 (day 0 = ovulation), representing metestrus (n = 10), early diestrus (n = 7), mid-diestrus (n = 9), and proestrus (n = 7), respectively. Regions of interest (ROIs) in each quadrant of the CL (which occupied at least 20% of the quadrant) were selected for analysis. Three MR image slices were analyzed: the slice with the greatest cross-sectional area of the CL, and the slices acquired immediately before and after that slice. The mean NPVs of the CL in T(1)-weighted images increased at each phase from metestrus (1,104 +/- 40 msec) to early diestrus (1,119 +/- 77 msec), to late diestrus (1,206 +/- 43 msec) and proestrus (1446 +/- 80 msec; P < 0.001). The mean NPVs in T(1)-weighted images were higher in regressing CL (proestrous) than in any other phase (P < 0.002). Grayscale heterogeneity of CL in T(1)-weighted images tended to increase during regression (P < 0.07). Regressing CL (proestrus) exhibited higher T(1)-weighted mean NPVs (P < 0.01) and tended to have greater heterogeneity (P < 0.06) than growing (metestrus) and mature (diestrus) CL, even though similar progesterone concentrations were observed. The increased brightness and heterogeneity of regressing CL in T(1)-weighted images appeared to be correlated with increased connective tissue and triglyceride content and decreased vascularity. It is anticipated that diagnostic markers for luteal viability and atresia in the in vitro bovine model will be applied to in vivo studies in women.
Hilton
Jennifer L
JL
Department of Obstetrics, Gynecology and Reproductive Sciences, College of Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Baerwald
Angela R
AR
Sarty
Gordon E
GE
Adams
Gregg P
GP
Pierson
Roger A
RA
D000818:Animals; D001808:Blood Vessels / Q000033:anatomy & histology / Q000254:growth & development; D002417:Cattle; D003238:Connective Tissue / Q000033:anatomy & histology / Q000254:growth & development; D003338:Corpus Luteum / Q000033:anatomy & histology* / Q000098:blood supply / Q000254:growth & development*; D004031:Diestrus / Q000502:physiology; D030762:Estrous Cycle / Q000502:physiology*; D005260:Female; D005496:Follicular Atresia / Q000502:physiology; D008279:Magnetic Resonance Imaging; D008686:Metestrus / Q000502:physiology; D011237:Predictive Value of Tests; D011359:Proestrus / Q000502:physiology; D011374:Progesterone / Q000097:blood; D015203:Reproducibility of Results; D014280:Triglycerides / Q000378:metabolism
D014280:Triglycerides; D011374:Progesterone