[Insertional inactivation of genes encoding eukaryotic type serine/threonine protein kinases in cyanobacterium Synechocystis sp. PCC 6803].
Synechocystis sp. PCC 6803 mutants, in which one of the eukaryotic-type serine/threonine protein kinase genes pknD, pknE, pknG, and pknH was inactivated, were obtained by insertion mutagenesis. None of these mutants differed phenotypically from the wild-type strain, indicating that the pknD, pknE, pknG, and pknH genes are not of crucial importance for the photoautotrophically grown cyanobacterium. Mutant with the inactivated pknE gene was resistant to L-methionine-D,L-sulfoximine and especially to methylamine. The resistance was neither due to the impaired transport of these compounds nor to the inhibition of the production of toxic gamma-glutamylmethylamide from methylamine. The data presented suggest that resistance to methylamine may be associated with alterations in the regulation of the glutamine synthetase system and that the PknE protein kinase may be involved in the regulation of nitrogen metabolism in the cyanobacterium studied.
Galkin
A N
AN
Department of Genetics, Faculty of Biology, Moscow State University, Vorob'evy gory, Moscow, 119899 Russia. galkin_1@mail.ru
Mikheeva
L E
LE
Shestakov
S V
SV
D000458:Cyanobacteria / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D024881:Drug Resistance, Bacterial; D020868:Gene Silencing; D008717:Methionine Sulfoximine / Q000494:pharmacology; D008744:Methylamines / Q000494:pharmacology; D016254:Mutagenesis, Insertional; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*
D008744:Methylamines; D008717:Methionine Sulfoximine; C027451:methylamine; D017346:Protein Serine-Threonine Kinases
Insertsionnaia inaktivatsiaa genov, kodiruiushchikh serin/treoninovye proteinkinazy éukarioticheskogo tipa u tsianobakterii Synechocystis sp. PCC 6803.