A fluorogenic dye activated by the staudinger ligation.
Specific labeling of biomolecules with biochemical and biophysical probes is a central element of proteomics research. Here we describe a coumarin-phosphine dye that undergoes activation of coumarin fluorescence upon Staudinger ligation with azides. Since azides can be metabolically incorporated into cellular proteins and oligosaccharides, this dye may be a useful tool for profiling proteins and their posttranslational modifications.
Lemieux
George A
GA
Center for New Directions in Organic Synthesis, Department of Chemistry, Howard Hughes Medical Institute, University of California, Berkeley, California 94720, USA.
De Graffenried
Christopher L
CL
Bertozzi
Carolyn R
CR
D000818:Animals; D001386:Azides / Q000737:chemistry*; D003374:Coumarins / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D007700:Kinetics; D051379:Mice; D010720:Phosphines / Q000737:chemistry*; D013762:Tetrahydrofolate Dehydrogenase / Q000032:analysis / Q000737:chemistry
D001386:Azides; D003374:Coumarins; D005456:Fluorescent Dyes; D010720:Phosphines; D013762:Tetrahydrofolate Dehydrogenase