Characterization of PfRhop148, a novel rhoptry protein of Plasmodium falciparum.
Rhoptries are apical organelles which play an important role in erythrocytic invasion. A Plasmodium falciparum cDNA clone, coding for a novel rhoptry protein PfRhop148, was obtained by antibody screening of a library. The deduced amino acid sequence consists of 1262 amino acids and is highly rich in Asn (22%). The Asn residues are distributed in clusters and in multiple units of repeats. Analysis of specific RNA transcript and protein showed that PfRhop148 was synthesized at around 21 h post-invasion. IFA and immunoelectron microscopic analyses revealed a rhoptry localization for the protein. The role of this protein in invasion and its relationship to the RhopH complex is now under further investigation.
Lobo
Cheryl-Ann
CA
Department of Molecular Parasitology, The Lindsley Kimball Research Institute, New York Blood Center, 310 E 67th St, New York, NY 10021, USA. cheryl_lobo@nybc.org
Rodriguez
Marilis
M
Hou
Guangpei
G
Perkins
Margaret
M
Oskov
Yelena
Y
Lustigman
Sara
S
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D003001:Cloning, Molecular; D005455:Fluorescent Antibody Technique; D006801:Humans; D008018:Life Cycle Stages; D008565:Membrane Proteins / Q000737:chemistry; D051379:Mice; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity* / Q000648:ultrastructure; D015800:Protozoan Proteins / Q000032:analysis* / Q000235:genetics* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D008565:Membrane Proteins; D015800:Protozoan Proteins; C117001:ROP 1 protein, Toxoplasma gondii