Psychromonas arctica sp. nov., a novel psychrotolerant, biofilm-forming bacterium isolated from Spitzbergen.
Using starch as a carbon source at a cultivation temperature of 4 degrees C, a number of Gram-negative, aerobic strains was isolated from sea-ice and sea-water samples collected at Spitzbergen in the Arctic. Analysis of the genetic diversity of the novel isolates by random amplification of polymorphic DNA (RAPD) and ERIC fingerprinting revealed a homogenic group of biofilm-forming bacteria that contained small extrachromosomal elements. As a representative of the group, strain Pull 5.3T, isolated from a sea-water sample, was used for detailed characterization. The results of phylogenetic analysis indicated that the newly isolated strain is a member of the gamma-subclass of the Proteobacteria and belongs to the genus Psychromonas. On the basis of DNA-DNA hybridization experiments, chemotaxonomic studies and phenotypic characterization, strain Pull 5.3T (=CECT 5674T =DSM 14288T) clearly represents a novel species, for which the name Psychromonas arctica sp. nov. is proposed.
Groudieva
Tatiana
T
Institute of Technical Microbiology, Technical University Hamburg-Harburg, Kasernenstr. 12, 21073 Hamburg, Germany.
Grote
Ralf
R
Institute of Technical Microbiology, Technical University Hamburg-Harburg, Kasernenstr. 12, 21073 Hamburg, Germany.
Antranikian
Garabed
G
Institute of Technical Microbiology, Technical University Hamburg-Harburg, Kasernenstr. 12, 21073 Hamburg, Germany.
D034241:Alteromonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D001110:Arctic Regions; D018441:Biofilms; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D020563:Gammaproteobacteria; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013696:Temperature
D004269:DNA, Bacterial