Tumor-infiltrating T cells with similar antigen binding sites in different mucosa-associated lymphoid tissue type B cell lymphomas.
B cell lymphomas of mucosa-associated lymphoid tissue (MALT)-type arise on the background of chronic inflammation due to either autoimmunity or infection at various sites of the organism. Characteristically the tumor-infiltrating T cells found in large numbers in MALT-type lymphomas are predominantly of CD4(+) phenotype and may have impact on tumor pathogenesis. To assess whether the chromosomal translocation t(11;18)(q21; q21) that is specific for at least a subset of MALT-type lymphomas may have an impact on tumor environment, we investigated the antigen binding sites of tumor-infiltrating T cells from one t(11;18)-positive tumor of the thyroid and one t(11;18)-negative tumor of the stomach. MHC Allelotyping was performed and revealed common alleles in HLA-DR and HLA-DQ loci. Cloning and sequencing of the complementary determining region 3 in V(beta2) chains of T cell receptors demonstrated the use of identical J(beta)segments in both patients, suggestive for recognition of the same antigen. We therefore suggest that tumor-infiltrating CD4(+) T cells are tightly integrated in MALT-type lymphoma immunoarchitecture irrespective of genetic background and localization of the tumors.
Seeberger
Harald
H
Institute of Pathology, University of Würzburg, Josef-Schneider-Strasse 2, Germany.
Knörr
Constanze
C
Mainhardt
Christian
C
Müller-Hermelink
Hans Konrad
HK
Greiner
Axel
A
D000595:Amino Acid Sequence; D001666:Binding Sites, Antibody / Q000235:genetics / Q000276:immunology; D002880:Chromosomes, Human, Pair 11; D002887:Chromosomes, Human, Pair 18; D002999:Clone Cells; D022801:Complementarity Determining Regions / Q000235:genetics / Q000276:immunology; D004273:DNA, Neoplasm / Q000032:analysis; D005434:Flow Cytometry; D006683:HLA-DQ Antigens / Q000235:genetics; D006684:HLA-DR Antigens / Q000235:genetics; D006801:Humans; D007124:Immunoenzyme Techniques; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology / Q000473:pathology*; D018442:Lymphoma, B-Cell, Marginal Zone / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012334:RNA, Neoplasm / Q000032:analysis; D013274:Stomach Neoplasms / Q000235:genetics / Q000276:immunology / Q000473:pathology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology / Q000473:pathology*; D013964:Thyroid Neoplasms / Q000235:genetics / Q000276:immunology / Q000473:pathology; D014178:Translocation, Genetic
D022801:Complementarity Determining Regions; D004273:DNA, Neoplasm; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D012334:RNA, Neoplasm