Effects of low intensity infrared laser radiation on the water transport in the isolated toad urinary bladder.
OBJECTIVE
The aim of this work was to study the effects of low intensity laser radiation on water transport in the toad bladder in vitro.


METHODS
The water flow through the membrane was measured gravimetrically in bag preparations of the membrane.


RESULTS
Laser radiation did not alter the water transport in the presence nor in the absence of vasopressin. In contrast, when the hemibladders were previously treated with vasopressin, the laser decreased by approximately 33.70% arginine-vasopressin (AVP)-mediated water transport. Laser radiation increased 3'5'-cyclic adenosine monophosphate (3'5'-cAMP) mediated water transport by approximately 23%. The association of laser radiation with indomethacin (IND) did not affect AVP-mediated water transport.


CONCLUSIONS
This data suggests that the laser may have two effects on AVP-mediated water transport: one inhibitory effect on 3'5'-cAMP synthesis by inhibiting the adenylate cyclase complex and another stimulatory effect by inhibiting nucleotide-phosphodiesterase activity. Our results also indicate that the laser does not interfere in the prostaglandins biosynthesis induced by AVP.
Franco
Wilson
W
Department of Physiological Sciences, Universidade Federal de São Carlos, São Carlos, São Paulo, Brazil. wfr@power.ufscar.br
Leite
Renner S
RS
Parizotto
Nivaldo A
NA
D000818:Animals; D001127:Arginine Vasopressin / Q000502:physiology; D001692:Biological Transport / Q000528:radiation effects; D002024:Bufo marinus; D007213:Indomethacin / Q000494:pharmacology; D028022:Low-Level Light Therapy; D008297:Male; D001743:Urinary Bladder / Q000528:radiation effects*; D014882:Water-Electrolyte Balance / Q000528:radiation effects
D001127:Arginine Vasopressin; D007213:Indomethacin