The impact of StuI digestion in situ on FISH to human chromosomes with satellite DNA probes.
Human metaphase chromosomes were digested with StuI and subsequently hybridized in situ using chromosome 9 alphoid DNA and classical satellite III DNA as probes. The data obtained suggest that it is not possible to establish a general rule regarding the cytological effects induced by restriction enzymes in particular chromosome regions and that a number of factors, such as DNA sequences, DNA-protein interaction and enzyme structure, play a role in determining such effects.
Nieddu
M
M
Dipartimento di Scienze Applicate ai Biosistemi, Sezione di Biologia e Genetica, Cittadella Universitaria, 09042 Monserrato, Italy.
Pichiri
G
G
Melis
V
V
Mezzanotte
R
R
D002899:Chromosomes, Human, Pair 9 / Q000235:genetics*; D015342:DNA Probes / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000235:genetics*; D004276:DNA, Satellite / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence
D015342:DNA Probes; D004276:DNA, Satellite; D004262:DNA Restriction Enzymes