title: [Study on the existence, distribution and bioaccumulation of chlorophenols in crucian carps].
abstract: Four representative chlorophenols (CPs) and their conjugates in fish tissues were determined by gas chromatography with electron capture detector(GC-ECD). Crucian carps were exposed to water contaminated with CPs or not contaminated water separately at room temperature(22 +/- 5 C) for 96 h. If the contaminated water samples are continuously replaced at 24 h interval, the concentration of CPs can be maintained constant throughout the experiment. The presence of fish will not have influence on the concentration of CPs. The experiment results showed that both free and conjugated CPs existed in fish tissues. The total amount of these chlorophenols in fish tissues is bile > liver and kidney > muscle. The proportion of conjugated CPs increases with the number of chlorine atoms in each compound, nevertheless, the proportion of conjugated CPs in PCP is less than that of TCP. Conjugated CPs in bile are composed of glucuronide and sulfate ester conjugates, and glucuronide conjugate is over 93%. The bio-accumulation of CPs in fish tissues is expressed by bio-concentration factors (BCF). The BCF abstained from free CPs and their sulfate ester conjugate in bile has no correlation with the partition coefficient of 1-octanol/water system(Kow). On the other hand, a good correlation is observed between BCF and Kow abstained from glucuronide conjugate and total amount of CPs(r > 0.96). The bioconcentration factors(BCF) of CPs in bile are 2.0 x 10(3)-6.3 x 10(3).
authors.affiliation: Institute of Environmental Health Monitoring, Chinese Academy of Preventive Medicine, Beijing 100021, China.
authors.forename: F
authors.initials: F
authors.lastname: Wang
authors.forename: S
authors.initials: S
authors.lastname: Lin
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000818:Animals; D002347:Carps / Q000378:metabolism*; D002733:Chlorophenols / Q000493:pharmacokinetics*; D014018:Tissue Distribution
chemical_list: D002733:Chlorophenols