title: Presence of a vanA-carrying pheromone response plasmid (pBRG1) in a clinical isolate of Enterococcus faecium.
abstract: Sex pheromone plasmids, frequently found in Enterococcus faecalis, have rarely been detected in Enterococcus faecium. pBRG1 is an approximately 50-kb vanA-carrying conjugative plasmid of an E. faecium clinical isolate (LS10) that is transferable to E. faecalis laboratory strains. In cell infection experiments, E. faecium LS10 exhibited remarkably high invasion efficiency and produced cytopathogenic effects in Caco-2 cell monolayers. Growth in the presence of sex pheromones produced by E. faecalis JH2-2 was found to cause self-aggregation of both E. faecium LS10 and E. faecalis JH-RFV(pBRG1) (a transconjugant obtained by transfer of pBRG1 to E. faecalis JH2-2) and to increase the cell adhesion and invasion efficiencies of both E. faecium LS10 and E. faecalis JH-RFV(pBRG1). Sex pheromone cCF10 caused clumping of E. faecalis OG1RF(pBRG1) (a transconjugant obtained by transfer of pBRG1 to E. faecalis OG1RF) at a concentration approximately 100-fold higher than the one required for the control strain E. faecalis OG1RF(pCF10). PCR products of the expected sizes were obtained with primers internal to aggregation substance genes of E. faecalis pheromone response plasmids pAD1, pPD1, and pCF10 and primers internal to ash701 of E. faecium pheromone plasmid pHKK701. These findings suggest that pBRG1 of E. faecium LS10 is a sex pheromone response plasmid.
authors.affiliation: Department of Microbiology and Biomedical Sciences, University of Ancona, 60131 Ancona, Italy.
authors.forename: Gloria
authors.initials: G
authors.lastname: Magi
authors.forename: Roberta
authors.initials: R
authors.lastname: Capretti
authors.forename: Claudia
authors.initials: C
authors.lastname: Paoletti
authors.forename: Marco
authors.initials: M
authors.lastname: Pietrella
authors.forename: Luigi
authors.initials: L
authors.lastname: Ferrante
authors.forename: Francesca
authors.initials: F
authors.lastname: Biavasco
authors.forename: Pietro Emanuele
authors.initials: PE
authors.lastname: Varaldo
authors.forename: Bruna
authors.initials: B
authors.lastname: Facinelli
mesh_terms: D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics*; D018938:Caco-2 Cells; D019729:Carbon-Oxygen Ligases / Q000235:genetics*; D003227:Conjugation, Genetic; D016984:Enterococcus faecium / Q000235:genetics* / Q000502:physiology; D006801:Humans; D009842:Oligopeptides / Q000494:pharmacology*; D010675:Pheromones / Q000494:pharmacology*; D010957:Plasmids
chemical_list: D001426:Bacterial Proteins; D009842:Oligopeptides; D010675:Pheromones; C071437:VanA ligase, Bacteria; C065937:aggregation substance, Enterococcus faecalis; C057280:peptide pheromone cCF10; D019729:Carbon-Oxygen Ligases