Different bone integration profiles of turned and acid-etched implants associated with modulated expression of extracellular matrix genes.
OBJECTIVE
The manner in which surface roughness of Implants affects bone-implant integration remains unknown. This study correlated morphologic profiles of bone-implant integration and extracellular matrix (ECM) gene expression in response to the placement of implants with different surface topographies.


METHODS
T-shaped hollow implants with turned and dual acid-etched (DE) surfaces were placed into rat femurs. A bone integration curve (BIC) was created from serial histomorphometric measurements within the implant chamber. The mRNA expression pattern of ECM genes in bone healing with or without implants was examined using reverse transcriptase-polymerase chain reaction.


RESULTS
At week 2, the BIC of the DE implant increased near the implant surface, whereas that of the turned implant decreased. The bone-to-implant contact rate of the DE Implant was 6- and 2.5-fold higher than that of the tuned Implant at weeks 2 and 4, respectively. A spatially standardized histomorphometry revealed that, at week 2, the DE implant had a greater bone volume than the turned implant in a zone near the implant, but not in zones distant from the implant surface. The DE implant evoked an accelerated mRNA expression for osteonectin and osteocalcin compared with the turned Implant, along with an up-regulated expression for bone sialoprotein II, collagen III, and integrins in initial healing stages up to week 1.


CONCLUSIONS
The results indicate that different histologic bone integration profiles associated with increased surface roughness may be explained, in part, by the modulated expression of the selected ECM-related genes. The data provide evidence supporting the fact that gene regulation occurs at local levels of implant surfaces in vivo.
Ogawa
Takahiro
T
The Jane and Jerry Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, Biomaterials and Hospital Dentistry, UCLA School of Dentistry, Los Angeles, California 90095-1668, USA. tack@dent.ucla.edu
Nishimura
Ichiro
I
D000704:Analysis of Variance; D000818:Animals; D024061:Collagen Type III / Q000096:biosynthesis / Q000235:genetics; D015921:Dental Implants; D017267:Dental Prosthesis Design; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis / Q000235:genetics*; D005269:Femur; D005786:Gene Expression Regulation; D020007:Implants, Experimental; D058574:Integrin-Binding Sialoprotein; D016023:Integrins / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D016348:Osseointegration / Q000235:genetics*; D015675:Osteocalcin / Q000096:biosynthesis / Q000235:genetics; D015676:Osteonectin / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012795:Sialoglycoproteins / Q000235:genetics; D018709:Statistics, Nonparametric; D013499:Surface Properties; D014025:Titanium
D024061:Collagen Type III; D015921:Dental Implants; D016326:Extracellular Matrix Proteins; C546873:Ibsp protein, rat; D058574:Integrin-Binding Sialoprotein; D016023:Integrins; D015676:Osteonectin; D012333:RNA, Messenger; D012795:Sialoglycoproteins; D015675:Osteocalcin; D014025:Titanium