title: Galactosylated chitosan as a synthetic extracellular matrix for hepatocytes attachment.
abstract: Galactose moiety as the hepatocyte anchorage was covalently coupled with chitosan for the development of synthetic extracellular matrix. Hepatocytes adhesion to galactosylated chitosan (GC)-coated polystyrene (PS) dish became as high as 94.7% after 2 h incubation whereas the hepatocytes adhesion to chitosan-coated PS dish was 69.1%, indication of galactose-specific recognition between GC molecules and asialoglycoprotein receptors of hepatocytes. The DNA synthesis of the hepatocytes adhered to GC-coated dish was increased in the presence of epidermal growth factor (EGF) at low concentration of GC (0.05 microg/ml) whereas the DNA synthesis of the hepatocytes adhered to GC-coated dish was decreased in the presence of EGF at high concentration of GC (5 microg/ml). The spreading shapes of the hepatocytes adhered to the surface in the presence of EGF at low concentration of GC (0.05 microg/ml) were enhanced than in the absence of EGF. The hepatocytes adhered to the surface at high concentration of GC (5 microg/ml) showed round shapes and exhibited many spheroid formation after 24 h in the presence of EGF.
authors.affiliation: School of Agricultural Biotechnology, Seoul National University, 103 Serdun-dong, Kwonsun-gu, Suwon 441-744, South Korea.
authors.forename: In-Kyu
authors.initials: IK
authors.lastname: Park
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Hwan-Jeong
authors.initials: HJ
authors.lastname: Jeong
authors.forename: Hee-Seung
authors.initials: HS
authors.lastname: Bom
authors.forename: Ichiro
authors.initials: I
authors.lastname: Harada
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Akaike
authors.forename: Su-Il
authors.initials: SI
authors.lastname: Kim
authors.forename: Chong-Su
authors.initials: CS
authors.lastname: Cho
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D048429:Cell Size / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry*; D048271:Chitosan; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis; D004305:Dose-Response Relationship, Drug; D005109:Extracellular Matrix / Q000737:chemistry / Q000502:physiology*; D005690:Galactose / Q000737:chemistry* / Q000494:pharmacology*; D022781:Hepatocytes / Q000737:chemistry / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*
chemical_list: D020099:Coated Materials, Biocompatible; D002686:Chitin; D048271:Chitosan; D005690:Galactose