[New method for the isolation of astroglial cells and oligodendrocytes].
Based on the different properties of cellular adhesions, developmental time-courses and growth pattern of astroglial cells and oligodendrocytes, a method of isolating glial cells in vitro was developed. The observations under inverted light microscope and scan electronic microscope identified that the morphology of isolated cells were consistent with the previous description. Immunocytochemical examination showed the expressions of markers, the glial fibrillary acidic protein (GFAP) of astroglia cells and the myelin basic protein (MBP) of oligodendrocytes were consistent with the isolated cells. All parameters showed that the isolated glial cells were pure, and the new methods were easy and reliable.
Qu
W
W
Department of Environmental Health, School of Public Health, West China University of Medical Sciences, Chengdu 610041, China.
Peng
X
X
Wu
D
D
Xiao
B
B
D000818:Animals; D001253:Astrocytes / Q000166:cytology* / Q000648:ultrastructure; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D009836:Oligodendroglia / Q000166:cytology* / Q000648:ultrastructure; D051381:Rats