title: Comparison of stool immunoassay with standard methods for detection of Helicobacter pylori infection in patients with upper-gastrointestinal bleeding of peptic origin.
abstract: OBJECTIVE
  To assess the accuracy of the determination of Helicobacter pylori infection by a stool immunoassay in patients with upper-gastrointestinal bleeding (UGB) of peptic origin, in comparison with the routine histological study, serology, rapid urease and 13C-breath tests.
  
  
  METHODS
  Sixty-eight patients with endoscopically proven UGB of peptic origin were included. The presence of H. pylori was considered when observed on histology or, if negative, by the positive indications of two of the remaining tests (serology, rapid urease,13C-breath test). The accuracy of stool immunoassay was estimated according to results obtained with other diagnostic methods.
  
  
  RESULTS
  Lesions causing gastrointestinal bleeding were 49 duodenal ulcers, 11 gastric ulcers, six pyloric channel ulcers, 13 acute lesions of the gastric mucosa, and 16 erosive duodenitis. H. pylori infection was present in 59 (86.76%) patients. Forty-one patients had received nonsteroidal anti-inflammatory drugs. The sensitivity and specificity of the diagnostic methods were 47.5% and 100% for the rapid urease test, 93% and 87.5% for the breath test, 86.4% and 77.7% for serology, 89.4% and 100% for histology, and 96.6% and 33.3% for the stool test.
  
  
  CONCLUSIONS
  The detection of H. pylori antigen in stools in patients with UGB of peptic origin has a good sensitivity (96.6%) but a low specificity (33.3%) for the diagnosis of H. pylori infection, which probably makes this test an inadequate tool in this setting if utilized alone.
authors.affiliation: Gastroenterology Unit, Department of Internal Medicine, University General Hospital, Miguel Hernández University, Alicante, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Griñó
authors.forename: Sonia
authors.initials: S
authors.lastname: Pascual
authors.forename: José
authors.initials: J
authors.lastname: Such
authors.forename: Juan A
authors.initials: JA
authors.lastname: Casellas
authors.forename: María
authors.initials: M
authors.lastname: Niveiro
authors.forename: Mariano
authors.initials: M
authors.lastname: Andreu
authors.forename: Jesús
authors.initials: J
authors.lastname: Sáez
authors.forename: José R
authors.initials: JR
authors.lastname: Aparicio
authors.forename: Emilio
authors.initials: E
authors.lastname: Griñó
authors.forename: Luis
authors.initials: L
authors.lastname: Compañy
authors.forename: Raquel
authors.initials: R
authors.lastname: Laveda
authors.forename: Miguel
authors.initials: M
authors.lastname: Pérez-Mateo
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000942:Antigens, Bacterial / Q000032:analysis*; D001944:Breath Tests; D005243:Feces / Q000382:microbiology*; D005260:Female; D016481:Helicobacter Infections / Q000150:complications / Q000175:diagnosis*; D016480:Helicobacter pylori / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D010438:Peptic Ulcer Hemorrhage / Q000382:microbiology*; D012680:Sensitivity and Specificity
chemical_list: D000942:Antigens, Bacterial