High-throughput protein crystallization.
High-throughput structural biology is a focus of a number of academic and pharmaceutical laboratories around the world. The use of X-ray crystallography in these efforts is critically dependent on high-throughput protein crystallization. The application of current protocols yields crystal leads for approximately 30% of the input proteins and well-diffracting crystals for a smaller fraction. Increasing the success rate will require a multidisciplinary approach that must invoke techniques from molecular biology, protein biochemistry, biophysics, artificial intelligence, and automation.
Hui
Raymond
R
Affinium Pharmaceuticals, 100 University Avenue, Ont., Toronto, Canada.
Edwards
Aled
A
D001331:Automation; D003460:Crystallization / Q000379:methods*; D030562:Databases, Protein; D011506:Proteins / Q000737:chemistry*; D013997:Time Factors
D011506:Proteins