title: Bio-artificial skin composed of gelatin and (1-->3), (1-->6)-beta-glucan.
abstract: Porous scaffolds composed of gelatin and beta-glucan were prepared using the freeze-drying method. The scaffold had an inter-connected pore structure with average pore size of 90-150 microm. Results for the contact angle and cell attachment revealed that a high gelatin content was suitable for cellular attachment and distribution in two- or three-dimensional fibroblast cultures, because the gelatin had acidic residues, and arginine-glycine-aspartic acid groups. To prepare a stratified wound dressing to mimic the normal human skin, fibroblasts and keratinocyte cells were isolated from a child's foreskin, and were co-cultured in gelatin/beta-glucan scaffolds were cross-linked using 1-ethyl-(3-3-dimethylaminopropyl) carbodiimide hydrochloride. An in vivo study showed that after 1 week, the artificial dermis containing the fibroblasts enhanced the re-epithelialization of a full-thickness skin defect rather than the acellular scaffold.
authors.affiliation: School of Chemical Engineering, College of Engineering, Hanyang University, Seoul 133-791, South Korea.
authors.forename: Sang Bong
authors.initials: SB
authors.lastname: Lee
authors.forename: Hyun Wook
authors.initials: HW
authors.lastname: Jeon
authors.forename: Young Woo
authors.initials: YW
authors.lastname: Lee
authors.forename: Young Moo
authors.initials: YM
authors.lastname: Lee
authors.forename: Kang Won
authors.initials: KW
authors.lastname: Song
authors.forename: Moon Hyang
authors.initials: MH
authors.lastname: Park
authors.forename: Young Soo
authors.initials: YS
authors.lastname: Nam
authors.forename: Hee Chang
authors.initials: HC
authors.lastname: Ahn
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division; D002648:Child; D046508:Culture Techniques / Q000379:methods; D020405:Dermis / Q000166:cytology* / Q000503:physiopathology; D005109:Extracellular Matrix / Q000737:chemistry*; D005347:Fibroblasts / Q000637:transplantation*; D005612:Freeze Drying; D005780:Gelatin / Q000737:chemistry*; D005936:Glucans / Q000737:chemistry*; D006801:Humans; D008567:Membranes, Artificial; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D017768:Skin, Artificial; D023822:Tissue Engineering / Q000379:methods*; D016896:Treatment Outcome; D014945:Wound Healing / Q000502:physiology; D014950:Wounds, Penetrating / Q000601:surgery*
chemical_list: D005936:Glucans; D008567:Membranes, Artificial; C033671:epiglucan; D005780:Gelatin