The envelope glycoprotein E2 is a determinant of cell culture tropism in ruminant pestiviruses.
Bovine viral diarrhoea virus (BVDV) isolates infect cultured Madin-Darby bovine kidney (MDBK) cells as efficiently as sheep kidney cells. In contrast, border disease virus (BDV) propagates poorly in MDBK cells but infects sheep cells very efficiently. The envelope glycoprotein E2 has been shown to be essential for virus infectivity. To explore the potential role of E2 in pestivirus host range in cell cultures, we engineered a chimeric BVDV with the E2 coding region from BDV. As expected, the BVDV-E2(bdv) chimera retained the ability of BDV to multiply in sheep cells but experienced a remarkable reduction in its ability to propagate and form plaques in MDBK, a phenotype that is characteristic of the E2 donor, BDV31 virus. Control chimeric BVDV bearing a type II E2 demonstrated that the heterologous E2 does not impair replication in MDBK or lamb cells. These results establish a role for E2 in determining the tropism of a pestivirus in cell culture.
Liang
Delin
D
Department of Veterinary & Biomedical Sciences, University of Nebraska-Lincoln, Fair Street & East Campus Loop, Lincoln, NE 68583-0905, USA.
Sainz
Ignacio Fernandez
IF
Department of Veterinary & Biomedical Sciences, University of Nebraska-Lincoln, Fair Street & East Campus Loop, Lincoln, NE 68583-0905, USA.
Ansari
Israrul H
IH
Department of Veterinary & Biomedical Sciences, University of Nebraska-Lincoln, Fair Street & East Campus Loop, Lincoln, NE 68583-0905, USA.
Gil
Laura H V G
LHVG
Department of Veterinary & Biomedical Sciences, University of Nebraska-Lincoln, Fair Street & East Campus Loop, Lincoln, NE 68583-0905, USA.
Vassilev
Ventzislav
V
Department of Veterinary & Biomedical Sciences, University of Nebraska-Lincoln, Fair Street & East Campus Loop, Lincoln, NE 68583-0905, USA.
Donis
Ruben O
RO
Department of Veterinary & Biomedical Sciences, University of Nebraska-Lincoln, Fair Street & East Campus Loop, Lincoln, NE 68583-0905, USA.
D000595:Amino Acid Sequence; D000818:Animals; D018083:Border disease virus / Q000472:pathogenicity*; D002417:Cattle; D002460:Cell Line; D001908:Diarrhea Viruses, Bovine Viral / Q000472:pathogenicity*; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012418:Ruminants / Q000821:virology*; D012756:Sheep; D013045:Species Specificity; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010948:Viral Plaque Assay; D015678:Viral Structural Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014774:Virulence
D006023:Glycoproteins; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins; D015678:Viral Structural Proteins; C085424:glycoprotein E2, border disease virus; C076551:gp53, bovine viral diarrhea virus