title: Loss of the Tg737 protein results in skeletal patterning defects.
abstract: Tg737 mutant mice exhibit pathologic conditions in numerous tissues along with skeletal patterning defects. Herein, we characterize the skeletal pathologic conditions and confirm a role for Tg737 in skeletal patterning through transgenic rescue. Analyses were conducted in both the hypomorphic Tg737(orpk) allele that results in duplication of digit one and in the null Tg737(delta2-3betaGal) allele that is an embryonic lethal mutation exhibiting eight digits per limb. In early limb buds, Tg737 expression is detected throughout the mesenchyme becoming concentrated in precartilage condensations at later stages. In situ analyses indicate that the Tg737(orpk) mutant limb defects are not associated with changes in expression of Shh, Ihh, HoxD11-13, Patched, BMPs, or Glis. Likewise, in Tg737(delta2-3betaGal) mutant embryos, there was no change in Shh expression. However, in both alleles, Fgf4 was ectopically expressed on the anterior apical ectodermal ridge. Collectively, the data argue for a dosage effect of Tg737 on the limb phenotypes and that the polydactyly is independent of Shh misexpression.
authors.affiliation: The University of Alabama at Birmingham, Department of Cell Biology, Birmingham, Alabama 35294, USA.
authors.forename: Qihong
authors.initials: Q
authors.lastname: Zhang
authors.forename: Noel S
authors.initials: NS
authors.lastname: Murcia
authors.forename: Laura R
authors.initials: LR
authors.lastname: Chittenden
authors.forename: William G
authors.initials: WG
authors.lastname: Richards
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Michaud
authors.forename: Richard P
authors.initials: RP
authors.lastname: Woychik
authors.forename: Bradley K
authors.initials: BK
authors.lastname: Yoder
mesh_terms: D000818:Animals; D015415:Biomarkers; D019521:Body Patterning; D001842:Bone and Bones / Q000002:abnormalities* / Q000033:anatomy & histology / Q000196:embryology / Q000502:physiology; D004622:Embryo, Mammalian / Q000002:abnormalities / Q000033:anatomy & histology / Q000502:physiology*; D005121:Extremities / Q000033:anatomy & histology / Q000196:embryology; D017930:Genes, Reporter; D053823:Hedgehog Proteins; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D009024:Morphogenesis; D010641:Phenotype; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D014070:Tooth / Q000033:anatomy & histology / Q000196:embryology; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D025521:Tumor Suppressor Proteins
chemical_list: D015415:Biomarkers; D053823:Hedgehog Proteins; D018398:Homeodomain Proteins; D011506:Proteins; C490898:Tg737Rpw protein, mouse; D015534:Trans-Activators; D025521:Tumor Suppressor Proteins