Histopathological and immunocytochemical studies of chlormadinone acetate on the rat prostate.
In order to confirm the relationship between glutathione-peroxidase (GSH-PO) and testosterone action in rat ventral prostate, we have studied the immunocytochemical localization of GSH-PO in glandular epithelial cells of rat ventral prostate after chlormadinone acetate CMA) as antiandrogen. In the control rat ventral prostate, GSH-PO was predominantly observed in the glandular epithelial cells and intracellular localization of GSH-PO was exclusively observed in the cytoplasmic matrix near the rough endoplasmic reticulum and it was occasionally noted as small granular structure. In CMA-administered rats, the glandular epithelial cells of the ventral prostate were markedly atrophic. The intensity of GSH-PO staining in the glandular epithelial cells was markedly decreased. Immunoelectron microscopically, GSH-PO-positive granules were hardly seen in the atrophic glandular epithelial cells. These findings strongly suggest that loss of GSH-PO staining in the glandular epithelial cells of the rat ventral prostate treated with CMA a s antiandrogen is thought to be caused by inhibition of testosterone action, and that its staining pattern is useful for the effect of antiandrogens or antiprostatic agents on prostate.
Murakoshi
Masanori
M
Safety Research Department, Teikoku Hormone Mfg. Co., Ltd, Kawasaki, Japan.
Ikeda
Rie
R
Fukui
Norio
N
Tagawa
Masashi
M
D000726:Androgen Antagonists / Q000494:pharmacology*; D000818:Animals; D002715:Chlormadinone Acetate / Q000494:pharmacology*; D004396:Coloring Agents; D004801:Eosine Yellowish-(YS); D005456:Fluorescent Dyes; D005979:Glutathione Peroxidase / Q000378:metabolism*; D006416:Hematoxylin; D007150:Immunohistochemistry; D008297:Male; D011467:Prostate / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling
D000726:Androgen Antagonists; D004396:Coloring Agents; D005456:Fluorescent Dyes; D002715:Chlormadinone Acetate; D005979:Glutathione Peroxidase; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin