Purification and some properties of lysostaphin, a glycylglycine endopeptidase from the culture liquid of Staphylococcus simulans biovar staphylolyticus.
This work presents a method for purification of lysostaphin, a glycylglycine endopeptidase, from the culture liquid of S. simulans biovar staphylolyticus to homogeneity in a few steps. The method includes ultrafiltration and ion-exchange and hydrophobic chromatographies. The enzyme was isolated in preparative amounts with the yield of 51%. Some physical and chemical properties of the enzyme are described.
Fedorov
T V
TV
State Research Center for Applied Microbiology, Ministry of Health of Russian Federation, Obolensk, Moscow Region, 142279, Russia. center@gncpm.serpukhov.su
Surovtsev
V I
VI
Pletnev
V Z
VZ
Borozdina
M A
MA
Gusev
V V
VV
D017077:Culture Media, Conditioned / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D057927:Hydrophobic and Hydrophilic Interactions; D008248:Lysostaphin / Q000737:chemistry* / Q000302:isolation & purification*; D013210:Staphylococcus / Q000201:enzymology*
D017077:Culture Media, Conditioned; D008248:Lysostaphin