Levan fructotransferase from Arthrobacter oxydans J17-21 catalyzes the formation of the di-D-fructose dianhydride IV from levan.
A new levan fructotransferase (LFTase) isolated from Arthrobacter oxydans J17-21 was characterized for the production of difructose dianhydride IV (DFA IV). LFTase was purified to apparent homogeneity by Q-Sepharose ion exchange chromatography, Mono-Q HR 5/5 column chromatography, and gel permeation chromatography. The enzyme had an apparent molecular mass of 54000 Da. The optimum pH for the enzyme-catalyzed reaction was pH 6.5, and the optimum temperature was observed at 45 degrees C. The LFTase was activated by the presence of CaCl(2) and EDTA-2Na but inhibited strongly by MnCl(2) and CuSO(4) at 1 mM and completely by FeSO(4) and Ag(2)SO(4) at 1 mM. A bacterial levan from Zymomonas mobilis was incubated with an LFTase; final conversion yield from the levan to DFA IV was 35%. Neither inulin, levanbiose, sucrose, dextran, nor starch was hydrolyzed by LFTase. DFA IV was very stable at acidic pH and high temperature, thus indicating that DFA IV may be suitable for the food industry and related areas.
Jang
Ki-Hyo
KH
Graduate School of East-West Medical Science, Kyung Hee University, Suwon 449-701, Korea.
Ryu
Eun-Ja
EJ
Park
Buem-Seek
BS
Song
Ki-Bang
KB
Kang
Soon Ah
SA
Kim
Chul Ho
CH
Uhm
Tai-Boong
TB
Park
Yong-Il
YI
Rhee
Sang-Ki
SK
D001173:Arthrobacter / Q000201:enzymology*; D002852:Chromatography, Ion Exchange; D004187:Disaccharides / Q000737:chemistry*; D005630:Fructans / Q000378:metabolism*; D006602:Hexosyltransferases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D013696:Temperature; D017355:Zymomonas / Q000737:chemistry
D004187:Disaccharides; D005630:Fructans; D011135:Polysaccharides, Bacterial; C033869:difructose anhydride IV; C072599:levan; D006602:Hexosyltransferases; C040074:levan fructotransferase