Membrane and synaptic effects of anti-S-100 are prevented by the same antibodies in low concentrations.
Effects of antiserum to S-100 protein (AS-100) in high (HC, corresponding to antiserum dilution 1:5 - 1:50) and low (LC, 1:10(12)) concentrations were studied in identified snail neurons and rat hippocampal slices. HC-AS-100 changed the frequency of action potential generation in spontaneously active neurons and blocked formation of long-term potentiation in mossy fiber synapses. LC-AS-100 alone did not affect any characteristic measured in our experiments, but 20 min pre-incubation of snail ganglia or hippocampal slices with LC-AS-100 abolished the effects of the same antibodies in HC. Simultaneous addition of both LC and HC did not prevent the development of HC-AS-100 effects. It seems possible that LC-AS-100 is capable of interaction with neuronal cells modifying their reaction to HC-AS-100.
Epstein
O I
OI
Materia Medica Holding Research and Production Company, Moscow, Russia.
Beregovoy
A
A
Sorokina
N S
NS
Starostina
M V
MV
Shtark
M B
MB
Gainutdinov
Kh L
KhL
Gainutdinova
T Kh
TKh
Muhamedshina
D I
DI
D000818:Animals; D001323:Autoantibodies / Q000502:physiology*; D004306:Dose-Response Relationship, Immunologic; D017774:Long-Term Potentiation / Q000502:physiology; D008564:Membrane Potentials / Q000276:immunology* / Q000502:physiology; D019599:Mossy Fibers, Hippocampal / Q000502:physiology; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D051381:Rats; D009418:S100 Proteins / Q000276:immunology* / Q000378:metabolism; D012908:Snails; D013569:Synapses / Q000276:immunology* / Q000502:physiology
D001323:Autoantibodies; D009418:S100 Proteins