Sensitivity of galanin- and melanin-concentrating hormone-containing neurones to nutritional status: an immunohistochemical study in the ovariectomized ewe.
The sensitivities of galanin and melanin-concentrating hormone (MCH) neuronal systems to nutrition are poorly understood in sheep compared to rodents. The aim of this study was to describe the changes in the numbers of galanin and MCH neurones in ovariectomized ewes submitted to different nutritional levels. In the first experiment, ewes were fed ad libitum or food deprived for 24 h. In the second experiment, two groups of ewes were fed at maintenance level (group 100) or undernourished (group 40) for 167 days, after which one-half of each group was killed or refed ad libitum (group 100R and 40R) for 4 days. The MCH neuronal population located in the lateral hypothalamic area was not affected by these nutritional changes. Long-term undernutrition enhanced the number of galanin neurones located in the infundibular nucleus and the dorsal hypothalamic area (DHA), refeeding resulted in an increase of neurones in the DHA and preoptic area, but short-term starvation had no effect on any galanin subpopulations. Our data suggest that the sensitivity of MCH neuronal populations to nutrition in sheep differs from that of rodents. Various populations of galanin-containing neurones differ in sensitivity in ewes subjected to long undernutrition and refeeding but not to short starvation.
Chaillou
E
E
INRA-PRC CNRS-6073, Nouzilly, France.
Baumont
R
R
Fellmann
D
D
Tramu
G
G
Tillet
Y
Y
D000818:Animals; D005260:Female; D005502:Food; D005508:Food Deprivation; D019004:Galanin / Q000032:analysis*; D007028:Hypothalamic Hormones / Q000032:analysis*; D007031:Hypothalamus / Q000166:cytology; D007150:Immunohistochemistry; D008543:Melanins / Q000032:analysis*; D009474:Neurons / Q000737:chemistry / Q000502:physiology*; D009752:Nutritional Status; D010052:Ovariectomy; D010907:Pituitary Hormones / Q000032:analysis*; D012756:Sheep / Q000502:physiology*; D013217:Starvation
D007028:Hypothalamic Hormones; D008543:Melanins; D010907:Pituitary Hormones; C019463:melanin-concentrating hormone; D019004:Galanin