Activity of Novispirin G-10, a novel antimicrobial peptide against Chlamydia trachomatis and vaginosis-associated bacteria.
We tested the activity of Novispirin G-10, a novel antimicrobial alpha-helical octadecapeptide structurally related to cathelicidins and other innate immunity peptides, against Chlamydia trachomatis serovars L2, D, and E and three organisms associated with bacterial vaginosis (BV). The peptide's activity against C. trachomatis was measured in 48-h shell vial assays with McCoy cell targets. Exposure to 100 micro g/ml of Novispirin G-10 reduced the infectivity of serovars D and E by 99.4-100% and serovar L2 by 91.7-99.1%. At the same concentration of 100 micro g/ml, Novispirin G-10 rapidly killed >99% of Mobiluncus curtisii, Gardnerella vaginalis, and Prevotella bivia, in standard colony-forming unit (CFU) assays. Given its simple structure and relative lack of cytotoxic and hemolytic activity, Novispirin G-10 may be a useful component of microbicide preparations designed to prevent chlamydial infection and/or remediate the abnormal vaginal flora associated with BV.
Yasin
B
B
Department of Pathology and Laboratory Medicine, University of California at Los Angeles School of Medicine, Los Angeles, CA 90095-1731, USA.
Pang
M
M
Lehrer
R I
RI
Wagar
E A
EA
D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use*; D023181:Antimicrobial Cationic Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use*; D016866:Bacteroidaceae Infections / Q000188:drug therapy; D002460:Cell Line; D002690:Chlamydia Infections / Q000188:drug therapy* / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000145:classification / Q000187:drug effects*; D003114:Colony-Forming Units Assay; D005260:Female; D006194:Gardnerella vaginalis / Q000187:drug effects; D006801:Humans; D008826:Microbial Sensitivity Tests; D017904:Mobiluncus / Q000187:drug effects; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use* / Q000652:urine; D018720:Prevotella / Q000187:drug effects; D017433:Protein Structure, Secondary; D012703:Serotyping; D016585:Vaginosis, Bacterial / Q000188:drug therapy* / Q000382:microbiology*
D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D010455:Peptides; C474629:novospirin G-10