Study of the interaction between daunorubicin and human serum albumin, and the determination of daunorubicin in blood serum samples.
The interactions between daunorubicin and human serum albumin (HSA) were studied using the spectrofluorimetric method. The binding constants of daunorubicin with HSA were obtained at different temperatures. The effects of various metal ions on the binding constants of daunorubicin with HSA were also studied. The optimum conditions of fluorometric determination of daunorubicin were studied and the developed method was successfully applied to the determination of daunorubicin in serum samples.
Jiang
Chong-Qiu
CQ
Department of Chemistry, Shandong Normal University, 250014, Jinan, China. gao_mx77@yahoo.com.cn <gao_mx77@yahoo.com.cn>
Gao
Ming-Xia
MX
Meng
Xian-Zhe
XZ
D001774:Blood Chemical Analysis / Q000379:methods; D003630:Daunorubicin / Q000097:blood*; D004735:Energy Transfer; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008670:Metals / Q000494:pharmacology; D011485:Protein Binding / Q000187:drug effects; D012709:Serum Albumin / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013696:Temperature
D008670:Metals; D012709:Serum Albumin; D003630:Daunorubicin