[Genomic organization and promoter analysis of mouse apoptosis signal-regulating kinase 2 (ASK 2)].
ASK 2/MAPKKK 6 was identified as a member of the MAPKKK family, which shares 45% amino acid identity with ASK 1/MAPKKK 5. In this study, the genomic organization and promoter of mouse ASK 2 were determined. ASK 2 gene spans approximately 12 kb and is comprised of 29 exons, flanked by 5' regulatory regions. Through comparison with ASK 2 cDNA variant clones, two splice variants were identified: a transcript lacking 24 bases encoding 8 amino acids was caused by disuse of exon 3, and another frameshifted transcript due to 4 bases deletion was produced by usage of the first 4 bases of exon 17 as a part of the splicing acceptor. Reporter plasmid analysis demonstrated that a promoter existed in the immediate upstream region adjacent to exon 1. Interestingly, the 5'-flanking region of the promoter possessed a strong suppressive element. These results suggest that expression of ASK 2 is regulated by multiple mechanisms.
Henmi
Maki
M
Department of Maxillofacial Reconstruction and Function, Division of Maxillofacial/Neck Reconstruction, Graduate School, Tokyo Medical and Dental University.
D000818:Animals; D005091:Exons; D040481:Genome Components; D007438:Introns; D020930:MAP Kinase Kinase Kinases; D051379:Mice; D011401:Promoter Regions, Genetic / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*
D017346:Protein Serine-Threonine Kinases; D020930:MAP Kinase Kinase Kinases; C116394:MAP kinase kinase kinase 6