A small yeast RNA inhibits HCV IRES mediated translation and inhibits replication of poliovirus in vivo.
OBJECTIVE
To investigate the anti-virus infection activity of internal ribosome entry site (IRES) specific inhibitor RNA (IRNA).


METHODS
IRNA eukaryotic vector pcRz-IRNA or mIRNA eukaryotic vector pcRz-mIRNA was transfected into human hepatocarcinoma cells (HHCC), then selected with neomycin G418 for 4 to 8 weeks, and then infected with polio virus vaccines line. The cytopethogenesis effect was investigated and the cell extract was collected. At last the polio virus titer of different cells was determined by plaque assay.


RESULTS
Constructive expression of IRNA was not detrimental to cell growth. HCV IRES-mediated cap-independent translation was markedly inhibited in cells constructively expressing IRNA compared to control hepatoma cells. However, cap-dependent translation was not significantly affected in these cell line. Additionally, HHCC cells constitutively expressing IRNA became refractory to infection of polio virus.


CONCLUSIONS
IRES specific IRNA can inhibit HCV IRES mediated translation and poliovirus replication.
Liang
Xue-Song
XS
The center of diagnosis and treatment for infectious diseases of Tangdu Hospital of Military Medical University of PLA, Xi'an. 710038, Shaanxi Province, China.
Lian
Jian-Qi
JQ
Zhou
Yong-Xing
YX
Nie
Qing-He
QH
Hao
Chun-Qiu
CQ
D020121:5' Untranslated Regions; D003588:Cytopathogenic Effect, Viral / Q000235:genetics; D015870:Gene Expression; D005814:Genes, Viral; D016174:Hepacivirus / Q000235:genetics*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics; D017955:Poliovirus / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology*; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics; D012331:RNA, Fungal / Q000235:genetics*; D034741:RNA, Small Interfering; D012367:RNA, Viral / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014162:Transfection; D014407:Tumor Cells, Cultured; D014779:Virus Replication / Q000235:genetics
D020121:5' Untranslated Regions; D035683:MicroRNAs; D012331:RNA, Fungal; D034741:RNA, Small Interfering; D012367:RNA, Viral; D012313:RNA