title: The role of glutathione in carboplatin ototoxicity in the chinchilla.
abstract: The role of glutathione in carboplatin ototoxicity was investigated in the chinchilla. Chinchillas hearing was tested with both distortion product otoacoustic emissions (DPOAE) and evoked potentials recorded from a chronic electrode in the inferior colliculus (IC). All subjects had an osmotic pump fitted to their right ear and it received buthionine sulfoximine (BSO) at a dose of 15 mM delivered at 5 ml per hour for 14 days. A group (N=4) was given a double dose of carboplatin (25 mg/kg i.p. for 2 days). The pump was implanted three days before the carboplatin dose. The BSO treated ears showed a greater loss in both evoked potential and DPOAE measures, as well as substantially fewer missing hair cells. The results implicate reactive oxygen species (ROS) as a common factor in ototoxic reactions because suppression of glutathione antioxidant leads to greater ototoxic reactions.
authors.affiliation: Center for Hearing and Deafness, State University of New York at Buffalo, 3435 Main Street, 215 Parker Hall, Buffalo, New York 14214 USA, Email: caltman@acsu.buffalo.edu
authors.forename: D.
authors.initials: D
authors.lastname: Henderson
authors.forename: B. H.
authors.initials: BH
authors.lastname: Hu
authors.forename: S. L.
authors.initials: SL
authors.lastname: McFadden
authors.forename: X. Y.
authors.initials: XY
authors.lastname: Zheng
authors.forename: D.
authors.initials: D
authors.lastname: Ding