An antibody raised against in vitro-derived human mast cells identifies mature mast cells and a population of cells that are Fc epsilon RI(+), tryptase(-), and chymase(-) in a variety of human tissues.
Selective markers for human mast cells are of paramount importance for understanding their role in physiological and pathological processes. A mouse monoclonal antibody (MAb) designated 2C7, raised against in vitro-derived human mast cells, was used in immunoenzymatic analysis of sections from a variety of human organs. Double immunolabeling with 2C7 and tryptase, chymase, Fc epsilon RIalpha, and c-kit was performed on cryostat tissue sections from skin, colon, uterus, breast, stomach, bladder, and lung. MAb 2C7 stained greater than 93% of the tryptase(+) or chymase(+) mast cells in all tissues examined. In addition, the majority of cells stained with the tryptase or chymase also stained for Fc epsilon RIalpha. However, there were a significant number of Fc epsilon RIalpha(1) cells in all tissues studied that were tryptase(-) and/or chymase(-). In contrast, MAb 2C7 in double immunoenzymatic staining co-localized with 93-96% of the Fc epsilon RIalpha(1) cells in all tissues. Analysis for c-kit expression on the different tissues revealed that the majority of tryptase(+) or chymase(+) cells in skin, uterus, bladder, and lung stained with c-kit. However, only approximately 70-78% of tryptase(+) cells in colon and stomach were c-kit(+). These data suggest that MAb 2C7 appears to identify mature mast cells and a population of Fc epsilon RIalpha(1), chymase(-), and tryptase(-) cells in a variety of human tissues.
Qi
Jian Cheng
JC
The Department of Medicine, The University of New South Wales, Australia.
Li
Lixin
L
Li
Yong
Y
Moore
Kate
K
Madigan
Michele C
MC
Katsoulotos
Gregory
G
Krilis
Steven A
SA
D000818:Animals; D000911:Antibodies, Monoclonal; D053818:Chymases; D005455:Fluorescent Antibody Technique; D005629:Frozen Sections; D006801:Humans; D006967:Hypersensitivity / Q000097:blood; D007124:Immunoenzyme Techniques; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D008407:Mast Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D018613:Microscopy, Confocal; D009928:Organ Specificity; D017455:Receptors, IgE / Q000276:immunology / Q000378:metabolism*; D012697:Serine Endopeptidases / Q000276:immunology / Q000378:metabolism*; D053802:Tryptases
D000911:Antibodies, Monoclonal; D017455:Receptors, IgE; C509190:Tpsb2 protein, mouse; D012697:Serine Endopeptidases; D053818:Chymases; C509222:Tpsab1 protein, mouse; D053802:Tryptases