Preparation and flow cytometry of uniform silica-fluorescent dye microspheres.
Uniform fluorescent silica-dye microspheres have been prepared by coating preformed monodispersed silica particles with silica layers containing rhodamine 6G or acridine orange. The resulting dispersions exhibit intense fluorescent emission between 500 and 600 nm, over a broad excitation wavelength range of 460 to 550 nm, even with exceedingly small amounts of dyes incorporated into the silica particles (10-30 ppm, expressed as weight of dye relative to weight of dry particles). The fluorescent particles can be prepared in micrometer diameters suitable for analyses using flow cytometry with 488-nm laser excitation.
Bele
Marjan
M
Center for Advanced Materials Processing, Clarkson University, Potsdam, New York, 13699-5814, USA.
Siiman
Olavi
O
Matijević
Egon
E