title: Effect of anti-tumor necrosis factor-alpha monoclonal antibody in alleviating renal ischemia-reperfusion injury.
abstract: OBJECTIVE
  To investigate the effect of anti-tumor necrosis factor-alpha monoclonal antibody (anti-TNF-alpha mAb) in alleviating renal ischemia-reperfusion injury.
  
  
  METHODS
  Fifty normal male Sprague-Dawley rats were randomly divided into 3 groups, namely group A that was subjected to ischemia-reperfusion injury with intravenous administration of anti-TNF-alpha mAb (0.1mg/kg.b.w.) 5 min before reperfusion (treatment group), group B with the same injury followed by saline administration in the same manner (control group), and group C with only anesthetization and leparotomy but not ischemia (sham operation group). Routine assays were performed for testing the levels of blood creatine (Cr), blood urea nitrogen (BUN), plasma TNF-alpha and the cell apoptosis. Ultrastructure of the kidney was also observed.
  
  
  RESULTS
  Renal ischemia-reperfusion resulted in significant increase of the levels of Cr, BUN and TNF-alpha in the plasma (P<0.01), but these effects were offset by administration of anti-TNF-alpha mAb (P<0.01). In group B, widespread pathological changes and cell apoptosis were observed in the renal tissue following renal ischemia-reperfusion injury, while similar changes were scarcely visible in group A due to the protective effect of intravenous administration of anti-TNF-alpha mAb 5 min before reperfusion.
  
  
  CONCLUSIONS
  Renal ischemia-reperfusion injury can be alleviated by anti-TNF-alpha mAb treatment.
authors.affiliation: Department of Kidney Transplantation, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
authors.forename: Shu-ling
authors.initials: SL
authors.lastname: Han
authors.forename: Li-xin
authors.initials: LX
authors.lastname: Yu
authors.forename: Jun-jie
authors.initials: JJ
authors.lastname: Ma
authors.forename: Fang-yin
authors.initials: FY
authors.lastname: Zeng
authors.forename: Xiao-you
authors.initials: XY
authors.lastname: Liu
authors.forename: Xu-yong
authors.initials: XY
authors.lastname: Sun
authors.forename: Hai-tao
authors.initials: HT
authors.lastname: Shan
authors.forename: Qian
authors.initials: Q
authors.lastname: Yuan
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D004195:Disease Models, Animal; D007511:Ischemia / Q000150:complications; D007674:Kidney Diseases / Q000150:complications; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000517:prevention & control*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D014409:Tumor Necrosis Factor-alpha