title: Backbone (15)N relaxation analysis of the N-terminal domain of the HTLV-I capsid protein and comparison with the capsid protein of HIV-1.
abstract: Human T-cell leukemia virus type 1 (HTLV-I) is an oncogenic retrovirus that exhibits specific tropism for human T-cells. The capsid (CA) proteins of retroviruses share highly conserved secondary and tertiary structures. However, they can form quaternary structures (assembled cores) that are conical (e.g., the lentivirus subgroup, including HIV) or spherical (e.g., the oncovirus subgroup, including HTLV). The intrinsic features that drive these differences are not understood. So far, only structural studies have been used as a basis for comparison. Dynamics may play a role in particle formation. High-resolution nuclear magnetic resonance (NMR) (15)N relaxation data (T(1), T(1rho), and NOE) have been used to characterize the backbone dynamics of the N-terminal domain (NTD) of the oncovirus HTLV-I and to compare with the CA NTD of HIV-1. Large variations in the (15)N heteronuclear NOEs and transversal relaxation rates for individual residues are consistent with the bundle RMSD of the previously calculated NMR structures. The beta-hairpin and CyP-A loop exhibit different mobility in HTLV-I and HIV-1. The overall hydrodynamic property of the HTLV-I capsid NTD is quite distinct from the HIV-1.
authors.affiliation: Laboratory of Biophysical Chemistry, National Heart, Lung, and Blood Institute, NIH, Bethesda, Maryland 20892, USA.
authors.forename: Claudia C
authors.initials: CC
authors.lastname: Cornilescu
authors.forename: Fadila
authors.initials: F
authors.lastname: Bouamr
authors.forename: Carol
authors.initials: C
authors.lastname: Carter
authors.forename: Nico
authors.initials: N
authors.lastname: Tjandra
mesh_terms: D036022:Capsid Proteins / Q000737:chemistry*; D015497:HIV-1 / Q000737:chemistry*; D015368:Human T-lymphotropic virus 1 / Q000737:chemistry*; D006801:Humans; D009038:Motion; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary
chemical_list: D036022:Capsid Proteins; D009587:Nitrogen Isotopes