title: Segmental tracheal replacement in mongrel dogs.
abstract: OBJECTIVE
  To evaluate the use of alcohol- and detergent-preserved tracheal allografts in dogs.
  
  
  METHODS
  Experimental segmental tracheal replacement was performed in 18 adult mongrel dogs. Three different techniques were tried in three groups of dogs. In the first group, a four-ring cervical tracheal segment was dissected out and implanted in the trachea of another dog. In the second group, the procedure was performed using a tracheal allograft that had been preserved in 70% ethyl alcohol for 20 days. In the third group, tracheal grafts were previously impregnated in 10% povidone iodine for 72 h before being implanted. Allografts were harvested 60 days after transplantation and assessed both histologically and in terms of the percentage patency. Dogs that died within 60 days were also included in the analysis.
  
  
  RESULTS
  The best results were found in the group in which the tracheal allograft had been preserved in ethyl alcohol and this was explained by the reduced antigenicity of the graft in this group.
  
  
  CONCLUSIONS
  The use of alcohol-preserved allografts is a practical method of tracheal transplantation, and the alcohol-preservation technique markedly reduces the immunogenicity of the grafts.
authors.affiliation: Department of Otorhinolaryngology, Faculty of Medicine, Alexandria University, Alexandria, Egypt. gaafar_a@hotmail.com
authors.forename: H
authors.initials: H
authors.lastname: Gaafar
authors.forename: A
authors.initials: A
authors.lastname: Hamza
authors.forename: M
authors.initials: M
authors.lastname: Hisham
authors.forename: S
authors.initials: S
authors.lastname: Helal
authors.forename: A
authors.initials: A
authors.lastname: Gaafar
authors.forename: M
authors.initials: M
authors.lastname: Reda
mesh_terms: D000818:Animals; D004285:Dogs; D004724:Endoscopy; D000431:Ethanol / Q000494:pharmacology; D006084:Graft Rejection; D006085:Graft Survival; D007150:Immunohistochemistry; D023421:Models, Animal; D009926:Organ Preservation / Q000379:methods*; D016377:Organ Transplantation / Q000379:methods* / Q000401:mortality; D012680:Sensitivity and Specificity; D015996:Survival Rate; D014132:Trachea / Q000473:pathology* / Q000637:transplantation*; D014184:Transplantation, Homologous
chemical_list: D000431:Ethanol