Permanent restoration of human skin treated with cultured epithelium grafting--wound healing by stem cell based tissue engineering--.
The technique of epidermal cell culture developed by Green and colleagues made a breakthrough in the treatment of massive wounds in vivo with grown cells in vitro. In the past two decades, progress of culture methods and clinical practice have been made and now it is possible to treat extensive skin defect with large amounts of cultured epithelium. Since 1985, we have been successfully used cultured epidermis as autografts for the permanent coverage of full-thickness burn wounds or excised burn scars, giant nevi, tattoos and so on. Furthermore, cultured epidermis has been available as allografts to promote the healing of chronic skin ulcers or deep dermal burn. In this paper we describe our clinical experience of cultured epithelium grafting for the treatment of wounds and predict new trial of wound management and regeneration based on tissue engineering concept.
Oshima
Hideo
H
Department of Plastic & Reconstructive Surgery, St. Marianna University School of Medicine, Kawasaki, Japan. h2oshima@marianna-u.ac.jp
Inoue
Hajime
H
Matsuzaki
Kyouichi
K
Tanabe
Masayoshi
M
Kumagai
Norio
N
D000328:Adult; D002056:Burns / Q000601:surgery*; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D062109:Dermatologic Surgical Procedures; D004847:Epithelial Cells / Q000637:transplantation*; D006801:Humans; D008297:Male; D013234:Stem Cells; D013653:Tattooing; D023822:Tissue Engineering; D014820:Vitiligo / Q000601:surgery; D014945:Wound Healing / Q000502:physiology*