[Inhibition by IFN-(Asp)4-Lys-HIV chimeric protein of hydrolysis of the low molecular substrate by the enteropeptidase light chain].
The full length enteropeptidase or it's light chain have often used for the limited proteolysis of recombinant chimeric proteins incorporating the linker-(Asp)4Lys- to obtain the target protein. Any chimeric proteins were not cleaved by the full length enteropeptidase efficiently. The resistant to the hydrolysis chimeric protein IFN-(Asp)4Lys-HIV earlier was shown to be the competitive inhibitor (Ki = 3,4 x 10(-6) M) in relation to the low molecular substrate. In present study we were determined this chimeric protein competitive inhibited the same substrate hydrolysis by enteropeptidase light chain (Ki = 2,7 x 10(-5) M). Comparison the Ki values for the substrate hydrolysis by full length enzyme and its light chain suggests that the enteropeptidase heavy chain may participate in chimeric protein binding.
Shibanova
E D
ED
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, GSP-7, Moscow, 117997 Russia. shib@enzyme.siobc.ras.ru
Grishina
Iu B
IuB
Rumsh
L D
LD
D001224:Aspartic Acid / Q000235:genetics*; D002384:Catalysis; D003864:Depression, Chemical; D004765:Enteropeptidase / Q000737:chemistry*; D016333:HIV Protease / Q000235:genetics*; D006868:Hydrolysis; D007371:Interferon-gamma / Q000235:genetics*; D007700:Kinetics; D008970:Molecular Weight; D009088:Mucoproteins / Q000235:genetics*; D009842:Oligopeptides / Q000737:chemistry*; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics
D009088:Mucoproteins; D009842:Oligopeptides; D011993:Recombinant Fusion Proteins; C010393:lysin, gastropoda; D001224:Aspartic Acid; C023519:glycyl-aspartyl-aspartyl-aspartyl-aspartyl-lysine-2-naphthylamide; D007371:Interferon-gamma; D004765:Enteropeptidase; D016333:HIV Protease
Ingibirovanie khimernym belkom IFN-(Asp)4-Lys-HIV gidroliza nizkomolekuliarnogo substrata legkoĭ tsep'iu énteropeptidazy.