Lipid membrane immobilized horseradish peroxidase biosensor for amperometric determination of hydrogen peroxide.
Stable films of didodecyldimethylammonium bromide (DDAB, a synthetic lipid) and horseradish peroxidase (HRP) were made by casting the mixture of the aqueous vesicle of DDAB and HRP onto the glassy carbon (GC) electrode. The direct electron transfer between electrode and HRP immobilized in lipid film has been demonstrated. The lipid films were used to supply a biological environment resembling biomembrane on the surface of the electrode. A pair of redox peaks attributed to the direct redox reaction of HRP were observed in the phosphate buffer solution (pH 5.5). The cathodic peak current increased dramatically while anodic peak decreased by addition of small amount H(2)O(2). The pH effect on amperometric response to H(2)O(2) was studied. The biosensor also exhibited fast response (5 s), good stability and reproducibility.
Tang
Jilin
J
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, People's Republic of China.
Wang
Bingquan
B
Wu
Zhengyan
Z
Han
Xiaojun
X
Dong
Shaojun
S
Wang
Erkang
E
D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry; D006735:Horseradish Peroxidase; D006861:Hydrogen Peroxide / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D008567:Membranes, Artificial; D010084:Oxidation-Reduction; D000644:Quaternary Ammonium Compounds
D008567:Membranes, Artificial; D000644:Quaternary Ammonium Compounds; C046112:didodecyldimethylammonium; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase