title: Screen-printed electrodes with electropolymerized Meldola Blue as versatile detectors in biosensors.
abstract: Electropolymerization of Meldola Blue was carried out by cyclic voltammetry in the range from -0.6 to +1.4 V vs. Ag/AgCl, thus defining a new immobilization procedure of the phenoxazine mediator on screen-printed graphite electrodes. Evidence of polymer formation was provided by electrochemical and Fourier transform infrared spectroscopy (FTIR) data. Following polymerization, Meldola Blue preserved the ability to catalyze NADH oxidation allowing to achieve a detection limit of 2.5 x 10(-6) mol l(-1) and a sensitivity of 3713 microA l mol(-1) in amperometric determinations at 0 V vs. Ag/AgCl. In addition, the polymeric mediator was found to facilitate the reduction of hydrogen peroxide in the absence of peroxidase. Typical calibration at -0.1 V vs. Ag/AgCl shows a detection limit of 8.5 x 10(-5) mol l(-1), a sensitivity of 494 microA l mol(-1) and a linear range from 2.5 x 10(-4) to 5 x 10(-3) mol l(-1) hydrogen peroxide.
authors.affiliation: Monitoring and Quality Control Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Bucharest, 4-12 Regina Elisabeta Blvd., 703461 -1, Bucharest, Romania.
authors.forename: Alina
authors.initials: A
authors.lastname: Vasilescu
authors.forename: Silvana
authors.initials: S
authors.lastname: Andreescu
authors.forename: Camelia
authors.initials: C
authors.lastname: Bala
authors.forename: Simona Carmen
authors.initials: SC
authors.lastname: Litescu
authors.forename: Thierry
authors.initials: T
authors.lastname: Noguer
authors.forename: Jean Louis
authors.initials: JL
authors.lastname: Marty
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry; D003067:Coenzymes / Q000737:chemistry; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006861:Hydrogen Peroxide / Q000032:analysis; D009243:NAD / Q000032:analysis* / Q000737:chemistry; D010078:Oxazines / Q000138:chemical synthesis / Q000737:chemistry*; D010781:Photography / Q000379:methods; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D020099:Coated Materials, Biocompatible; D003067:Coenzymes; D010078:Oxazines; D011108:Polymers; D009243:NAD; C005559:Meldola blue; D006861:Hydrogen Peroxide