title: Comparison of cytotoxicity of aminoglycoside antibiotics using a panel cellular biotest system.
abstract: The cytotoxicity of four aminoglycoside antibiotics was studied by estimation of the dose-effect relationship using a panel cellular biotest system including cell cultures for test objects. The cultures represented 4 differentiation types: normal human fibroblasts and myoblasts, human or Syrian hamster hepatoma cells, and mouse/mouse hybridoma cells. It was found that three widely used antibiotics gentamicin, kanamycin, and neomycin exhibit similar, but not identical cytotoxicity parameters and differ distinctly from geneticin. Hence, the proposed panel biotest system helps to quantitatively evaluate and differentiate the effects of bioactive substances with similar chemical structure.
authors.affiliation: Medical Genetic Research Center, Russian Academy of Medical Sciences, Moscow.
authors.forename: V G
authors.initials: VG
authors.lastname: Chernikov
authors.forename: S M
authors.initials: SM
authors.lastname: Terekhov
authors.forename: T B
authors.initials: TB
authors.lastname: Krokhina
authors.forename: S S
authors.initials: SS
authors.lastname: Shishkin
authors.forename: T D
authors.initials: TD
authors.lastname: Smirnova
authors.forename: E A
authors.initials: EA
authors.lastname: Kalashnikova
authors.forename: N V
authors.initials: NV
authors.lastname: Adnoral
authors.forename: L B
authors.initials: LB
authors.lastname: Rebrov
authors.forename: Yu I
authors.initials: YI
authors.lastname: Denisov-Nikol'skii
authors.forename: V A
authors.initials: VA
authors.lastname: Bykov
mesh_terms: D000617:Aminoglycosides / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002461:Cell Line, Transformed; D002470:Cell Survival / Q000187:drug effects; D005347:Fibroblasts / Q000473:pathology*; D006801:Humans; D032446:Myoblasts / Q000473:pathology*
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents