Beacon interacts with cdc2/cdc28-like kinases.
Previously we found elevated beacon gene expression in the hypothalamus of obese Psammomys obesus. Beacon administration into the lateral ventricle of P. obesus stimulated food intake and body weight gain. In the current study we used yeast two-hybrid technology to screen for proteins in the human brain that interact with beacon. CLK4, an isoform of cdc2/cdc28-like kinase family of proteins, was identified as a strong interacting partner for beacon. Using active recombinant proteins and a surface plasmon resonance based detection technique, we demonstrated that the three members of this subfamily of kinases (CLK1, 2, and 4) all interact with beacon. Based on the known sequence and functional properties of beacon and CLKs, we speculate that beacon could either modulate the function of key regulatory molecules such as PTP1B or control the expression patterns of specific genes involved in the central regulation of energy metabolism.
Kantham
Lakshmi
L
Metabolic Research Unit, School of Health Sciences, Deakin University, Waurn Ponds 3217, Vic., Australia. kantham@deakin.edu.au <kantham@deakin.edu.au>
Kerr-Bayles
Lyndal
L
Godde
Nathan
N
Quick
Melissa
M
Webb
Ryan
R
Sunderland
Terry
T
Bond
Judy
J
Walder
Ken
K
Augert
Guy
G
Collier
Greg
G
D001921:Brain / Q000378:metabolism; D016203:CDC2 Protein Kinase / Q000145:classification; D017489:CDC28 Protein Kinase, S cerevisiae / Q000145:classification; D018844:Cyclin-Dependent Kinases / Q000378:metabolism*; D004734:Energy Metabolism; D006801:Humans; D009419:Nerve Tissue Proteins; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D020349:Surface Plasmon Resonance; D020798:Two-Hybrid System Techniques; D014452:Ubiquitins
D009419:Nerve Tissue Proteins; D011506:Proteins; D014452:Ubiquitins; C498170:Ubl5 protein, rat; C066780:Clk dual-specificity kinases; D011505:Protein-Tyrosine Kinases; D017346:Protein Serine-Threonine Kinases; D016203:CDC2 Protein Kinase; D017489:CDC28 Protein Kinase, S cerevisiae; D018844:Cyclin-Dependent Kinases