Metabolically engineered methylotrophic yeast cells and enzymes as sensor biorecognition elements.
An extended definition of the term metabolic engineering is given and its successful use in the construction of biorecognition elements of sensors is demonstrated. It is shown that genetic and chemical modifications of methylotrophic yeast cells provide directed changes in their physiological responses towards methanol, ethanol and formaldehyde resulting in enhanced selectivity and shorter time response of the corresponding potentiometric and amperometric biosensors.
Gonchar
Mykhailo
M
Department of Molecular Genetics and Biotechnology, Institute of Cell Biology, Drahomanov Street 14/16, Lviv 79005, Ukraine. gonchar@biochem.lviv.ua
Maidan
Mykola
M
Korpan
Yaroslav
Y
Sibirny
Volodymyr
V
Kotylak
Zbigniew
Z
Sibirny
Andrei
A
D001698:Biomedical Engineering; D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry; D006863:Hydrogen-Ion Concentration; D008660:Metabolism; D015003:Yeasts / Q000235:genetics / Q000378:metabolism*