title: Protein adsorption, fibroblast activity and antibacterial properties of poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) grafted with chitosan and chitooligosaccharide after immobilized with hyaluronic acid.
abstract: Poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV) membrane was treated with ozone and grafted with acrylic acid. The resulting membranes were further grafted with chitosan (CS) or chitooligosaccharide (COS) via esterification. Afterward hyaluronic acid (HA) was immobilized onto CS- or COS-grafting membranes. The antibacterial activity of CS and COS against Staphylococus aureus, Escherichia coli, and Pseudomonas aeruginosa was preserved after HA immobilization. Among them, CS-grafted PHBV membrane showed higher antibacterial activity than COS-grafted PHBV membrane. In addition, after CS- or COS-grafting, the L929 fibroblasts attachment and protein adsorption were improved, while the cell number was decrease. After immobilizing HA, the cell proliferation was promoted, the protein adsorption was decreased, and the cell attachment was slightly lower than CS- or COS-grafting PHBV.
authors.affiliation: Department of Polymer Engineering, National Taiwan University of Science and Technology, 43, SEC.4 Keelung Road, Taipei 10672, Taiwan.
authors.forename: S-G
authors.initials: SG
authors.lastname: Hu
authors.forename: C-H
authors.initials: CH
authors.lastname: Jou
authors.forename: M C
authors.initials: MC
authors.lastname: Yang
mesh_terms: D020341:Absorbable Implants; D000327:Adsorption; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology*; D048271:Chitosan; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects* / Q000502:physiology*; D006820:Hyaluronic Acid / Q000737:chemistry; D008422:Materials Testing; D008567:Membranes, Artificial; D051379:Mice; D008826:Microbial Sensitivity Tests; D009844:Oligosaccharides / Q000737:chemistry / Q000494:pharmacology*; D011091:Polyesters / Q000737:chemistry*; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods; D000094122:Polyhydroxybutyrates
chemical_list: D000900:Anti-Bacterial Agents; D002686:Chitin; D048271:Chitosan; D020099:Coated Materials, Biocompatible; D006820:Hyaluronic Acid; D008567:Membranes, Artificial; D009844:Oligosaccharides; D011091:Polyesters; D011506:Proteins; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates