title: Determination of salivary peroxidase activity in human mixed whole saliva.
abstract: Mixed whole saliva contains salivary peroxidase (SPX) and myeloperoxidase (MPO), thus it is important to discriminate between the two peroxidases in order to understand their functions in the oral cavity. We developed a method to measure SPX activity in mixed whole saliva using an oxygen electrode. According to our results, when 50% of the peroxidase activity in saliva was due to MPO, determined using a typical substrate for peroxidase guaiacol, almost all oxygen evolved was due to SPX. We propose that measurement of H(2)O(2)-dependent oxygen evolution is a useful method for determining SPX activity in whole saliva.
authors.affiliation: Department of Pediatric Dentistry, Kyushu Dental College, Kitakyushu 803-8580, Japan. nishioka@kyu-dent.ac.jp
authors.forename: Takahiro
authors.initials: T
authors.lastname: Nishioka
authors.forename: Kenshi
authors.initials: K
authors.lastname: Maki
authors.forename: Mitsutaka
authors.initials: M
authors.lastname: Kimura
authors.forename: Umeo
authors.initials: U
authors.lastname: Takahama
mesh_terms: D004563:Electrochemistry; D004566:Electrodes; D006801:Humans; D006861:Hydrogen Peroxide; D010100:Oxygen / Q000032:analysis*; D009195:Peroxidase / Q000032:analysis*; D012463:Saliva / Q000201:enzymology*
chemical_list: D006861:Hydrogen Peroxide; D009195:Peroxidase; D010100:Oxygen