[Detection of fungi from agricultural commodities with serological method].
The types of fungus antigen and the purification of fungus antigen, the purification of anti-fungus antibody, and the application of serology in detecting fungi from agricultural commodities are introduced. Fungi antigen are mainly from extra-cellular protein and extra-cellular polysaccharide. The method for antigen purification was the same as the common method used to separate and purify proteins and polysaccharides. The specificity of an antibody is the most important parameter for the quality of antibody. As long as the specificity of a antibody is high, the antibody could detect the target fungi from agricultural commodities.
Chen
F
F
Department of Food Science, Huazhong Agricultural University, Wuhan 430070, China.
Li
G
G
Chen
J
J
Gan
Z
Z
D000818:Animals; D000908:Antibodies, Fungal / Q000276:immunology; D000946:Antigens, Fungal / Q000032:analysis*; D005506:Food Contamination; D005658:Fungi / Q000302:isolation & purification*; D012275:Oryza / Q000382:microbiology; D010945:Plants, Edible / Q000382:microbiology*
D000908:Antibodies, Fungal; D000946:Antigens, Fungal