[A method for measuring the strength of adhesion of Candida albicans on epithelial cells].
A method on estimation of Candida albicans adhesion to epitheliocytes is proposed. The principle consists in the fact that while centrifuging epitheliocytes (1000 cycles/min, 10 min) with adhered Candida albicans through phycoll-verografin (1.077 g/cm3 density), Candida albicans cells having poor adhesion are separated from epitheliocytes trying to rise to the surface of phycoll-verografin. Candida albicans strongly adhered to epitheliocytes settle down to the bottom of test tube. In experiments with buccal epitheliocytes of healthy donors the number of strongly adhered Candida albicans ranged from 13% to 50% and they amounted to 37.0 +/- 7.8% on an average.
Maianskií
A N
AN
Salina
E V
EV
Zaslavskaia
M I
MI
D002176:Candida albicans / Q000502:physiology*; D002448:Cell Adhesion; D002499:Centrifugation, Density Gradient; D002610:Cheek; D004847:Epithelial Cells / Q000382:microbiology*; D005362:Ficoll; D009061:Mouth Mucosa / Q000382:microbiology*
D005362:Ficoll
Sposob otsenki prochnosti adgezii Candida albicans na épiteliotsitakh.