title: Molecular cloning and characterization of the Delta9 fatty acid desaturase gene and its promoter region from Saccharomyces kluyveri.
abstract: A gene encoding Delta9 fatty acid desaturase, which catalyzes the first reaction of fatty acid desaturation, has been cloned from Saccharomyces kluyveri, a species capable of producing polyunsaturated fatty acids (poly-UFA). The open reading frame of this gene (named Sk-OLE1) consists of 1443 bp, encoding 480 amino acids. The deduced Sk-OLE1 protein shared 63-74% identity with those from other yeasts. Expression of the Sk-OLE1 gene in Saccharomyces cerevisiae complemented genetic disruption of the S. cerevisiae OLE1 gene (Sc-OLE1). Fatty acid analysis showed that the ratio of palmitoleic acid to oleic acid in the Sk-OLE1-expressing S. cerevisiae strain was lower than that of the S. cerevisiae strain expressing the Sc-OLE1 gene. The promoter region of the Sk-OLE1 gene was shorter than that of the Sc-OLE1 gene and appeared to lack the fatty acid-regulated sequences present in the Sc-OLE1 promoter region. Analysis of transcriptional expression revealed that the Sk-OLE1 gene was not actually repressed in either S. kluyveri or S. cerevisiae by exogenous UFAs. The DDBJ accession number for the sequence reported in this paper is AB071696.
authors.affiliation: Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan. skajiwar@bio.titech.ac.jp
authors.forename: Susumu
authors.initials: S
authors.lastname: Kajiwara
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D044943:Fatty Acid Desaturases / Q000235:genetics* / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012440:Saccharomyces / Q000201:enzymology / Q000235:genetics*; D013230:Stearoyl-CoA Desaturase
chemical_list: D005231:Fatty Acids, Unsaturated; D044943:Fatty Acid Desaturases; D013230:Stearoyl-CoA Desaturase; C029627:delta-9 fatty acid desaturase