title: Oxidative stress in erythrocytes from premature and full-term infants during their first 72 h of life.
abstract: OBJECTIVE
  The aim of this study was to evaluate the extent of lipid peroxidation and the response of the enzymatic and non-enzymatic antioxidant defence system in erythrocytes from full-term and premature infants at birth, after 3 and after 72 h of life.
  
  
  METHODS
  Twenty infants were selected and divided in two groups according to their gestational age. Blood samples were taken at birth, at 3 and at 72 h of life, erythrocytes were isolated and the following parameters were measured: fatty-acid profile, coenzyme Q, alpha-tocopherol, hydroperoxides and the activity of the antioxidant enzymes catalase, superoxide dismutase (SOD) and cytosolic glutathione peroxidase (cGPx).
  
  
  RESULTS
  For the three studied periods, several differences between full-term and premature infants were found. Premature children showed a higher concentration of hydroperoxides, a lower level of alpha-tocopherol and lower SOD and cGPx activity (except for cGPx at birth). Moreover, n-3 polyunsaturated fatty-acids percentages (essential for good neonatal development) were higher in full term children throughout all the study.
  
  
  CONCLUSIONS
  Results suggest a strong imbalance between oxidants and antioxidants in premature infants during their first 72 h of life, a situation which could lead to several pathologies. Therefore, further research is needed, including possible nutritional intervention (with antioxidant therapy, supplementation of essential fatty acids and other dietary constituents) before and after birth.
authors.affiliation: Institute of Nutrition and Food Technology (INYTA), Granada, Spain. jjoh@ugr.es
authors.forename: Julio J
authors.initials: JJ
authors.lastname: Ochoa
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Ramirez-Tortosa
authors.forename: José L
authors.initials: JL
authors.lastname: Quiles
authors.forename: Narcisa
authors.initials: N
authors.lastname: Palomino
authors.forename: Rafael
authors.initials: R
authors.lastname: Robles
authors.forename: José
authors.initials: J
authors.lastname: Mataix
authors.forename: Jesús R
authors.initials: JR
authors.lastname: Huertas
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D001034:Apgar Score; D001835:Body Weight; D002374:Catalase / Q000097:blood; D003600:Cytosol / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D005865:Gestational Age; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007231:Infant, Newborn; D007234:Infant, Premature; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism; D013482:Superoxide Dismutase / Q000097:blood; D013997:Time Factors; D014451:Ubiquinone / Q000097:blood; D024502:alpha-Tocopherol / Q000097:blood / Q000378:metabolism
chemical_list: D000975:Antioxidants; D005227:Fatty Acids; D014451:Ubiquinone; D006861:Hydrogen Peroxide; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D024502:alpha-Tocopherol; D010100:Oxygen