Multicolor fluorescence in situ hybridization studies in multiple myeloma and monoclonal gammopathy of undetermined significance.
The aim of the study was to test the multicolor fluorescence in situ hybridization technique (M-FISH) in seven multiple myeloma (MM) and eight monoclonal gammopathy (MGUS) patients. None of the eight MGUS patients had chromosomal abnormalities by conventional cytogenetics. In two of these patients structural abnormalities of chromosomes 2, 11 and 19 were found by M-FISH. However, these findings were not confirmed by conventional in situ hybridization. M-FISH only showed numerical chromosomal abnormalities in one out of the three MM cases with a normal karyotype. In the two MM cases with complex karyotype, M-FISH demonstrated the origin of the marker chromosomes. M-FISH is a useful technique to identify the origin of the marker chromosomes in MM. In contrast, MM or MGUS patients with normal karyotypes by conventional cytogenetics did not show structural abnormalities by M-FISH.
Gutiérrez
Norma C
NC
Servicio de Hematología, Hospital Universitario de Salamanca, Universidad de Salamanca-CSIC, Spain.
Camps
Jordi
J
Hernández
Jesús M
JM
García
Juan L
JL
Prat
Esther
E
González
M Belén
MB
Miró
Rosa
R
San Miguel
Jesús F
JF
D016477:Artifacts; D002869:Chromosome Aberrations; D020223:Chromosome Painting; D002877:Chromosomes, Human / Q000648:ultrastructure*; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D007621:Karyotyping; D008297:Male; D009101:Multiple Myeloma / Q000235:genetics* / Q000473:pathology; D010265:Paraproteinemias / Q000235:genetics* / Q000473:pathology; D012680:Sensitivity and Specificity