Site-selective RNA scission at two sites for precise genotyping of SNPs by mass spectrometry.
Short RNA fragments containing single nucleotide polymorphism (SNP) sites have been selectively clipped out of substrate RNA by using complementary DNA having two acridine residues and Lu(III), and the genotype of the substrate is accurately and easily determined by mass analysis of these fragments.
Kuzuya
Akinori
A
Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
Mizoguchi
Ryo
R
Morisawa
Fumi
F
Komiyama
Makoto
M
D000166:Acridines / Q000737:chemistry; D001057:Apolipoproteins E / Q000235:genetics; D001483:Base Sequence; D001665:Binding Sites; D018076:DNA, Complementary / Q000737:chemistry; D005091:Exons; D005838:Genotype; D006801:Humans; D006868:Hydrolysis; D008187:Lutetium / Q000737:chemistry; D020641:Polymorphism, Single Nucleotide; D012313:RNA / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013379:Substrate Specificity
D000166:Acridines; D001057:Apolipoproteins E; D018076:DNA, Complementary; D008187:Lutetium; D012313:RNA