title: Second-generation integrated genetic linkage/radiation hybrid maps of the domestic cat (Felis catus).
abstract: We report construction of second-generation integrated genetic linkage and radiation hybrid (RH) maps in the domestic cat (Felis catus) that exhibit a high level of marker concordance and provide near-full genome coverage. A total of 864 markers, including 585 coding loci (type I markers) and 279 polymorphic microsatellite loci (type II markers), are now mapped in the cat genome. We generated the genetic linkage map utilizing a multigeneration interspecies backcross pedigree between the domestic cat and the Asian leopard cat (Prionailurus bengalensis). Eighty-one type I markers were integrated with 247 type II markers from a first-generation map to generate a map of 328 loci (320 autosomal and 8 X-linked) distributed in 47 linkage groups, with an average intermarker spacing of 8 cM. Genome coverage spans approximately 2,650 cM, allowing an estimate for the genetic length of the sex-averaged map as 3,300 cM. The 834-locus second-generation domestic cat RH map was generated from the incorporation of 579 type I and 255 type II loci. Type I markers were added using targeted selection to cover either genomic regions underrepresented in the first-generation map or to refine breakpoints in human/feline synteny. The integrated linkage and RH maps reveal approximately 110 conserved segments ordered between the human and feline genomes, and provide extensive anchored reference marker homologues that connect to the more gene dense human and mouse sequence maps, suitable for positional cloning applications.
authors.affiliation: Laboratory of Genomic Diversity, National Cancer Institute, Frederick, MD 21702, USA.
authors.forename: M
authors.initials: M
authors.lastname: Menotti-Raymond
authors.forename: V A
authors.initials: VA
authors.lastname: David
authors.forename: M E
authors.initials: ME
authors.lastname: Roelke
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Chen
authors.forename: K A
authors.initials: KA
authors.lastname: Menotti
authors.forename: S
authors.initials: S
authors.lastname: Sun
authors.forename: A A
authors.initials: AA
authors.lastname: Schäffer
authors.forename: J F
authors.initials: JF
authors.lastname: Tomlin
authors.forename: R
authors.initials: R
authors.lastname: Agarwala
authors.forename: S J
authors.initials: SJ
authors.lastname: O'Brien
authors.forename: W J
authors.initials: WJ
authors.lastname: Murphy
mesh_terms: D000818:Animals; D002415:Cats / Q000235:genetics*; D003433:Crosses, Genetic; D016133:Polymerase Chain Reaction; D021101:Radiation Hybrid Mapping