title: Purification and characterization of complement-activating acidic polysaccharides from the fruits of Capsicum annuum.
abstract: Hot water-soluble crude polysaccharide (HCAP-0) that was obtained from the fruits of Capsicum annuum showed potent anti-complementary activity. The activity was unchanged by pronase digestion, but decreased by periodate oxidation. The HCAP-0 was fractionated by DEAE ion-exchange chromatography to give two major fractions, HCAP-II and III. These two fractions were finally purified by gel filtration to give HCAP-IIa, HCAPIIIa1, and IIIa2 fractions that had high anti-complementary activities. The HCAP-IIIa1 and IIIa2 consisted of homogeneous polysaccharides. The anti-complementary activities were unaffected by treatment with polymyxin B, indicating that the modes of complement activation were not due to preexisting lipopolysaccharide. The molecular weight and sugar content of HCAP-IIIa2 had potent anti-complementary activity. The highest yields were 55 kDa and 75.9%, and the molar ratio of galactose (Ara:Gal, 1.0:4.6) was higher than other sugars. The crossed immuno-electrophoresis showed that both classical and alternative pathways were activated by HCAP-IIIa2.
authors.affiliation: Department of Microbiology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
authors.forename: Soon-Young
authors.initials: SY
authors.lastname: Paik
authors.forename: Kyung Soo
authors.initials: KS
authors.lastname: Ra
authors.forename: In Seop
authors.initials: IS
authors.lastname: Chang
authors.forename: Yoon Chang
authors.initials: YC
authors.lastname: Park
authors.forename: Hee Sung
authors.initials: HS
authors.lastname: Park
authors.forename: Hyung Suk
authors.initials: HS
authors.lastname: Baik
authors.forename: Jong Won
authors.initials: JW
authors.lastname: Yun
authors.forename: Jang Won
authors.initials: JW
authors.lastname: Choi
mesh_terms: D001419:Bacteria / Q000737:chemistry; D002212:Capsicum / Q000737:chemistry*; D003167:Complement Activation; D005638:Fruit / Q000737:chemistry; D006801:Humans; D008070:Lipopolysaccharides / Q000737:chemistry; D011112:Polymyxin B / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D008070:Lipopolysaccharides; D011134:Polysaccharides; D011112:Polymyxin B