Lack of association of lipoprotein lipase gene polymorphisms with coronary artery disease in the Saudi Arab population.
BACKGROUND
Previous studies reported an association of certain polymorphisms in the lipoprotein lipase (LPL) gene with the risk of coronary artery disease (CAD); however, these studies were small and inconsistent. In addition, none of these studies attempted to establish such an association in the Arab population.


OBJECTIVE
To determine whether 2 LPL polymorphisms (LPL-HindIII and LPL-PvuII located on introns 8 and 6, respectively, of the LPL gene) can be considered as independent risk factors or as predictors for CAD in Arabs.


METHODS
We used polymerase chain reaction and restriction enzyme digestion to determine the distribution of the LPL-HindIII and LPL-PvuII polymorphisms among healthy blood donors of Arabic origin (BD group) and angiographically confirmed CAD patients (CAD group) with identical ethnic backgrounds.


RESULTS
For the HindIII genotypes, within the BD group (n = 410), the +/+ genotype was found in 206 individuals (50.2%), 173 (42.2%) carried the +/- genotype, and 31 (7.6%) carried the -/- genotype. Within the CAD group (n = 352), the +/+ genotype was found in 189 individuals (53.7%), 138 (39.2%) carried the +/- genotype, and 25 (7.1%) carried the -/- genotype. P values of.38,.45, and.92 were obtained for the +/+, +/-, and -/- genotypes, respectively. For the PvuII genotypes, within the BD group (n = 511), the +/+ genotype was found in 182 individuals (35.6%), 248 (48.5%) carried the +/- genotype, and 81 (15.9%) carried the -/- genotype. Within the CAD group (n = 431), the +/+ genotype was found in 138 individuals (32%), 225 (52.2%) carried the +/- genotype, and 68 (15.8%) carried the -/- genotype. P values of.28,.29, and.98 were obtained for the +/+, +/-, and -/- genotypes, respectively. The distribution and the allele frequency of these 2 LPL variants were similar in CAD and BD study groups and followed the Hardy-Weinberg equilibrium.


CONCLUSIONS
There was no difference in the distribution of both LPL polymorphisms between the healthy group and the CAD group. Therefore, these 2 LPL polymorphisms cannot be considered as independent risk factors or as predictors for CAD in this population.
Abu-Amero
Khaled K
KK
Department of Genetics, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia. kamero@kfshrc.edu.sa
Wyngaard
Carol A
CA
Al-Boudari
Olyan M
OM
Kambouris
Marios
M
Dzimiri
Nduna
N
D000328:Adult; D018912:Arabs / Q000235:genetics*; D003324:Coronary Artery Disease / Q000201:enzymology / Q000453:epidemiology / Q000235:genetics*; D015247:Deoxyribonuclease HindIII / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D006580:Genetic Carrier Screening; D005828:Genetics, Population / Q000379:methods*; D005838:Genotype; D006801:Humans; D007438:Introns / Q000235:genetics; D008071:Lipoprotein Lipase / Q000235:genetics*; D008297:Male; D011110:Polymorphism, Genetic / Q000235:genetics*; D011237:Predictive Value of Tests; D012307:Risk Factors; D012529:Saudi Arabia / Q000453:epidemiology
D008071:Lipoprotein Lipase; D015247:Deoxyribonuclease HindIII; C025630:CAGCTG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific