[Analysis of sperm chromosomes in infertile males with teratozoospermia].
OBJECTIVE
The aim of the study was to examine sperm chromosomes from infertile males with teratozoospermia.


METHODS
Basic research study


METHODS
Spermatozoa were obtained from infertile males with teratozoospermia. Intracytoplasmic sperm injection was used to inject human spermatozoa into mouse oocytes in order to examine sperm chromosomes.


RESULTS
Chromosomes of spermatozoa obtained from 6 infertile teratozoospermic and 1 fertile normozoospermic males were investigated. The mean frequency of structural chromosome aberrations in amorphous, motile spermatozoa was similar to that of morphologically normal sperm (9 vs. 7.7%). The structural aberrations found in amorphous, immotile spermatozoa reached 93% and revealed severity was significant.


CONCLUSIONS
Teratozoospermia per se did not increase the frequency of structural chromosome aberrations in sperm. Severe chromosomal damage was revealed when immotile spermatozoa were injected into oocytes by ICSI.
Szczygieł
Monika A
MA
Zespołu Biologii Rozrodu, Zakładu Genetyki Człowieka PAN.
Kusakabe
Hirokazu
H
Wiland
Ewa
E
Kurpisz
Maciej
M
D000328:Adult; D016022:Case-Control Studies; D002869:Chromosome Aberrations; D006801:Humans; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D009845:Oligospermia / Q000235:genetics*; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa / Q000648:ultrastructure*
Analiza chromosomów plemników od pacjentów z teratozoospermia.