title: Kinetics of inhibition of ribonuclease P activity by peptidyltransferase inhibitors. Effect of antibiotics on RNase P.
abstract: A cell-free system derived from Dictyostelium discoideum has been used to study the kinetics of inhibition of RNase P by puromycin, amicetin and blasticidin S. Detailed kinetic analysis showed that the type of inhibition of RNase P activity by puromycin is simple competitive, whereas the type of inhibition by amicetin and blasticidin S is simple non-competitive. On the basis of Ki values amicetin is stronger inhibitor than puromycin and blasticidin S.
authors.affiliation: Department of Biochemistry, School of Medicine, University of Patras, 26500 Patras, Greece.
authors.forename: Dimitra
authors.initials: D
authors.lastname: Kalavrizioti
authors.forename: Anastassios
authors.initials: A
authors.lastname: Vourekas
authors.forename: Apostolos
authors.initials: A
authors.lastname: Tekos
authors.forename: Antigoni
authors.initials: A
authors.lastname: Tsagla
authors.forename: Constantinos
authors.initials: C
authors.lastname: Stathopoulos
authors.forename: Denis
authors.initials: D
authors.lastname: Drainas
mesh_terms: D000818:Animals; D004023:Dictyostelium / Q000201:enzymology*; D004305:Dose-Response Relationship, Drug; D004722:Endoribonucleases / Q000037:antagonists & inhibitors*; D007700:Kinetics; D009705:Nucleosides / Q000378:metabolism / Q000493:pharmacokinetics; D010458:Peptidyl Transferases / Q000037:antagonists & inhibitors*; D011500:Protein Synthesis Inhibitors / Q000493:pharmacokinetics; D011691:Puromycin / Q000493:pharmacokinetics; D011741:Pyrimidine Nucleosides / Q000493:pharmacokinetics; D016337:RNA, Catalytic / Q000037:antagonists & inhibitors*; D043262:Ribonuclease P
chemical_list: D009705:Nucleosides; D011500:Protein Synthesis Inhibitors; D011741:Pyrimidine Nucleosides; D016337:RNA, Catalytic; C004413:amicetin; D011691:Puromycin; C004500:blasticidin S; D010458:Peptidyl Transferases; D004722:Endoribonucleases; D043262:Ribonuclease P