title: First in vitro directed biosynthesis of new compounds by a minimal type II polyketide synthase: evidence for the mechanism of chain length determination.
abstract: The minimal actinorhodin polyketide synthase bearing two point mutations (KSbeta Q161A, ACP C17S) was chemically modified to carry novel C4 to C8 starter units on the ACP: on incubation with an excess of malonyl CoA new 16-carbon polyketides are made, supporting a measuring mechanism.
authors.affiliation: School of Chemistry, University of Bristol, Cantock's Close, Bristol, UK BS8 1TS.
authors.forename: T P
authors.initials: TP
authors.lastname: Nicholson
authors.forename: C
authors.initials: C
authors.lastname: Winfield
authors.forename: J
authors.initials: J
authors.lastname: Westcott
authors.forename: J
authors.initials: J
authors.lastname: Crosby
authors.forename: T J
authors.initials: TJ
authors.lastname: Simpson
authors.forename: R J
authors.initials: RJ
authors.lastname: Cox
mesh_terms: D000213:Acyl Carrier Protein / Q000378:metabolism; D000217:Acyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000880:Anthraquinones / Q000737:chemistry / Q000378:metabolism*; D001426:Bacterial Proteins; D008316:Malonyl Coenzyme A / Q000378:metabolism; D048630:Polyketide Synthases; D021241:Spectrometry, Mass, Electrospray Ionization; D013302:Streptomyces / Q000201:enzymology; D000095622:Benzoisochromanequinones
chemical_list: D000213:Acyl Carrier Protein; D000217:Acyltransferases; D000880:Anthraquinones; D001426:Bacterial Proteins; D008316:Malonyl Coenzyme A; D048630:Polyketide Synthases; C087193:polyketide beta-ketoacylsynthase; C013390:actinorhodin; D000095622:Benzoisochromanequinones