title: FR252921, a novel immunosuppressive agent isolated from Pseudomonas fluorescens no. 408813. I. Taxonomy, fermentation, isolation, physico-chemical properties and biological activities of FR252921, FR252922 and FR256523.
abstract: Novel immunosuppressive agents, FR252921, FR252922 and FR256523 were isolated from the cultured broth of a bacterial strain No. 408813. The strain was identified Pseudomonas fluorescens from morphological and physiological characteristics. FR252921, FR252922 and FR256523, novel compounds containing macrolactone ring, showed immunosuppressive activity against murine splenocyte proliferation stimulated with lipopolysaccharide (LPS) or anti-CD3 mAb in vitro.
authors.affiliation: Exploratory Research Laboratories, Fujisawa Pharmaceutical Co., Ltd., 5-2-3 Tokodai, Tsukuba, Ibaraki 300-2698, Japan. kiyotaka_fujine@po.fujisawa.co.jp
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Fujine
authors.forename: Miho
authors.initials: M
authors.lastname: Tanaka
authors.forename: Keisuke
authors.initials: K
authors.lastname: Ohsumi
authors.forename: Michizane
authors.initials: M
authors.lastname: Hashimoto
authors.forename: Shigehiro
authors.initials: S
authors.lastname: Takase
authors.forename: Hirotsugu
authors.initials: H
authors.lastname: Ueda
authors.forename: Motohiro
authors.initials: M
authors.lastname: Hino
authors.forename: Takashi
authors.initials: T
authors.lastname: Fujii
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D005260:Female; D005285:Fermentation; D007166:Immunosuppressive Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D007783:Lactones / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D008970:Molecular Weight; D019906:Nuclear Magnetic Resonance, Biomolecular; D009904:Optical Rotation; D011551:Pseudomonas fluorescens / Q000737:chemistry / Q000145:classification / Q000378:metabolism* / Q000502:physiology; D012988:Soil Microbiology; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology
chemical_list: D007166:Immunosuppressive Agents; D007783:Lactones