Changes in lymphocyte subsets in children with newly diagnosed type 1 diabetes mellitus.
The aim of this study was to assess changes in selected peripheral blood lymphocyte subsets in children and adolescents with newly diagnosed type 1 diabetes mellitus (DM) and determine the correlation between these changes and other immunological markers. The study involved a group of 39 patients aged 2-14 years and a control group. The number of T- and B-lymphocytes and the number of CD4, CD8, CD4/HLA-DR, CD8/HLA-DR, CD5/CD20 subsets were measured by flow-cytometry using monoclonal antibodies. Islet cell antibodies (ICA) and antibodies to glutamic acid decarboxylase (GADA) were assessed. In both the diabetic and control groups the number of T-and B-lymphocytes were within normal limits. In patients with DM, the percentage of CD5+/CD20+ cells was significantly increased compared with the control group (p < 0.0001). ICA were positive in 80% of patients and GADA in nearly 65%. A positive correlation between the CD5/CD20 subset and ICA and GADA was found. In patients with a high percentage of CD5+/CD20+ lymphocytes, a higher percentage of activated subsets (CD4/HLA DR and CD8/ HLA DR) was detected.


CONCLUSIONS
CD5/ CD20 lymphocyte subsets are a good additional marker of autoimmunological processes in DM.
Kadziela
Katarzyna
K
Department of Paediatric Endocrinology, Medical University of Warsaw, Poland. endokrynologia@litewska.edu.pl
Kowalska
Halina
H
Rymkiewicz-Kluczyńska
Barbara
B
Kowalska
Małgorzata
M
Miszkurka
Grazyna
G
Rybczyńska
Jolanta
J
Wasik
Maria
M
Pańkowska
Ewa
E
D018941:Antigens, CD19; D018951:Antigens, CD20; D001402:B-Lymphocytes; D015415:Biomarkers; D015704:CD4 Antigens / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes; D018956:CD5 Antigens; D018414:CD8-Positive T-Lymphocytes; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology*; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D005968:Glutamate Decarboxylase / Q000378:metabolism; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D018655:Lymphocyte Count; D016131:Lymphocyte Subsets / Q000201:enzymology / Q000276:immunology*; D008297:Male
D018941:Antigens, CD19; D018951:Antigens, CD20; D015415:Biomarkers; D015704:CD4 Antigens; D018956:CD5 Antigens; D006684:HLA-DR Antigens; D005968:Glutamate Decarboxylase