title: Pharmacologically regulated induction of silent mutations (PRISM): combined pharmacological and genetic approaches for learning and memory.
abstract: Mouse transgenic and knock-out approaches have made fundamental contributions to our understanding of the molecular and cellular bases of learning and memory. These approaches have successfully identified a large number of molecules with either a central or modulatory role in learning and memory. However, there are limitations associated with first-generation mutant mice, which include, for example, the lack of temporal control over the mutation. Recent technical developments have started to address some of these shortcomings. Here, the authors review a newly developed inducible approach that takes advantage of synergistic interactions between subthreshold genetic and pharmacological manipulations. This approach is easily set up and can be used to study the functional interactions between molecules in signaling pathways.
authors.affiliation: Department of Neurobiology, University of California, Los Angeles, USA.
authors.forename: Paul W
authors.initials: PW
authors.lastname: Frankland
authors.forename: Masuo
authors.initials: M
authors.lastname: Ohno
authors.forename: Eiki
authors.initials: E
authors.lastname: Takahashi
authors.forename: Adele R
authors.initials: AR
authors.lastname: Chen
authors.forename: Rui M
authors.initials: RM
authors.lastname: Costa
authors.forename: Steven A
authors.initials: SA
authors.lastname: Kushner
authors.forename: Alcino J
authors.initials: AJ
authors.lastname: Silva
mesh_terms: D000818:Animals; D011905:Genes, ras / Q000235:genetics; D007858:Learning / Q000502:physiology*; D008568:Memory / Q000502:physiology*; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics; D008967:Molecular Biology / Q000379:methods*; D009154:Mutation; D009473:Neuronal Plasticity / Q000502:physiology; D010600:Pharmacology
chemical_list: D020928:Mitogen-Activated Protein Kinases