title: Inhibitory Effects of Dietary Monoglucosyl-rutin on Azoxymethane-induced Colon Carcinogenesis in Rats.
abstract: The dietary effect of monoglucosyl-rutin (M-R), a flavonoid, on azoxymethane (AOM)-induced colon carcinogenesis was investigated in two experiments with 5 week old, F344 male rats. In the first experiment (5 weeks study), effects of MR on AOM (15 mg/kg body weight 3 times weekly)-induced formation of aberrant crypt foci (ACF) in five groups were assessed. In this experiment, group 3 given 500 ppm M-R with AOM had a significantly smaller number of ACF containing 4 or more aberrant crypts than group 1 with AOM alone, and groups 2 and 3 given 100 ppm or 500 ppm M-R respectively had significantly lower BrdU labeling indices in the epithelial cells of large bowel than group 1. For the second experiment, rats were divided into 8 groups. Groups 1-5 were given AOM as in the first experiment. Groups 2-5 were fed diets containing 100ppm or 500ppm M-R for 4 weeks in the initiation phase or 36 weeks in the post-initiation phase. Group 6 was given 500ppm M-R throughout the experiment, and group 7 was kept on the basal diet and served as a control. At the termination of the experiment (40 weeks after the start), groups 2-5 had significantly smaller numbers of positive cells with anti-proliferating cell nuclea antigen (PCNA) antibody than group 1. Furthermore, group 5 treated with 500ppm M-R for 36 weeks demonstrated tendencies for decrease in the incidence and multiplicity of colon tumors. These data suggest that M-R has the potential to inhibit AOM-induced colon carcinogenesis.
authors.affiliation: First Department of Pathology of Gifu University School of Medicine, Gifu 500-8705 Japan. kengo@cc.gifu-u.ac.jp
authors.forename: Kengo
authors.initials: K
authors.lastname: Matsunaga
authors.forename: Naoki
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authors.lastname: Yoshimi
authors.forename: Kayoko
authors.initials: K
authors.lastname: Shimoi
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Yamada
authors.forename: Masaki
authors.initials: M
authors.lastname: Katayama
authors.forename: Keiko
authors.initials: K
authors.lastname: Sakata
authors.forename: Toshiya
authors.initials: T
authors.lastname: Kumo
authors.forename: Koujiro
authors.initials: K
authors.lastname: Yoshida
authors.forename: Zheng
authors.initials: Z
authors.lastname: Qiao
authors.forename: Naohide
authors.initials: N
authors.lastname: Kinae
authors.forename: Hideki
authors.initials: H
authors.lastname: Mori