Hadamard transform microchip electrophoresis combined with diode laser fluorometry.
The application of a Hadamard transform technique to microchip electrophoresis is described. The sample is electrokinetically injected into a separation channel and is then detected by diode laser-induced fluorometry. The sample and buffer solutions are introduced into the channel by controlling the high voltages applied to the solutions, according to a code determined by a Hadamard matrix. The S/N ratio of the signal in the electropherogram can be improved by a factor of 5 in comparison with that obtained by a conventional single-injection method, although an 8-fold improvement is theoretically predicted when a 255-order matrix is used.
Hata
Kazuki
K
Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Hakozaki, Fukuoka, 812-8581 Japan.
Kichise
Yasuhiko
Y
Kaneta
Takashi
T
Imasaka
Totaro
T