title: Epinephrine modulates cellular distribution of muscle phosphofructokinase.
abstract: In this paper, we report evidences that cellular distribution of phosphofructokinase can be affected by epinephrine stimulation in rabbit skeletal muscle homogenates. Through co-sedimentation techniques, we observed that in epinephrine-stimulated tissues, approximately 50% of phosphofructokinase activity is co-located in an actin-enriched fraction, against 29% in control. This phenomenon is accompanied by a 400% increase in specific phosphofructokinase activity in stimulated homogenates. This effect is reproduced by the beta-adrenergic agonist isoprenaline. Here we propose that the modulation of cellular distribution of phosphofructokinase may be one of the mechanisms of control of glycolytic flux in mammalian muscle, by beta-adrenergic stimulation.
authors.affiliation: Laboratório de Enzimologia e Controle do Metabolismo (LabECoM), Departamento de Fármacos, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Gutemberg
authors.initials: G
authors.lastname: Gomes Alves
authors.forename: Mauro
authors.initials: M
authors.lastname: Sola-Penna
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D004837:Epinephrine / Q000378:metabolism / Q000494:pharmacology*; D007545:Isoproterenol / Q000494:pharmacology; D018482:Muscle, Skeletal / Q000378:metabolism; D009132:Muscles / Q000201:enzymology*; D010732:Phosphofructokinase-1 / Q000096:biosynthesis* / Q000737:chemistry* / Q000378:metabolism; D011817:Rabbits; D013347:Subcellular Fractions; D014018:Tissue Distribution
chemical_list: D000199:Actins; D010732:Phosphofructokinase-1; D007545:Isoproterenol; D004837:Epinephrine