Multicentre evaluation of a new assay for determination of carbohydrate-deficient transferrin.
OBJECTIVE
The analytical performance of the new Tina-quant % carbohydrate-deficient transferrin (%CDT) was assessed in a multicentre study on Roche/Hitachi analysers.


METHODS
Intra-assay/total precision studies revealed median coefficients of variation (CVs) of 4.7/7.4% within the sites. Precision between the sites was proven using a serum panel.


RESULTS
Inter-laboratory CVs from 6.3 to 10.7% were obtained. The results of the participating laboratories compared well with high-performance liquid chromatography-UV technique fulfilling the criteria of a reference method for %CDT determination (slope 1.03, intercept -0.09% CDT, correlation 0.984). Good agreement was also found with the Axis-Shield %CDT microtitre test.


CONCLUSIONS
Data from this study indicate that reliable, well standardized %CDT results are obtained using the new assay.
Schwarz
Markus J
MJ
Psychiatrische Klinik der Ludwig-Maximilian-Universität, München, Germany.
Domke
Ingrid
I
Helander
Anders
A
Janssens
Pim M W
PM
Van Pelt
Johannes
J
Springer
Beate
B
Ackenheil
Manfred
M
Bernhardt
Klaus
K
Weigl
Gerhard
G
Soyka
Michael
M
D015415:Biomarkers / Q000032:analysis; D016000:Cluster Analysis; D005069:Evaluation Studies as Topic; D006801:Humans; D007118:Immunoassay / Q000379:methods* / Q000592:standards; D008297:Male; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D044967:Serum / Q000737:chemistry*; D014168:Transferrin / Q000031:analogs & derivatives* / Q000032:analysis*
D015415:Biomarkers; D011933:Reagent Kits, Diagnostic; D014168:Transferrin; C056759:carbohydrate-deficient transferrin