Biosynthesis of Vitamin B(6) in yeast. Incorporation pattern of trioses.
The biosynthetic origin of the C(3) unit, C-6,5,5', of pyridoxamine was investigated in two yeasts, Candida utilis ATCC 9256 and Saccharomyces cerevisiae ATCC 7752. The incorporation patterns within pyridoxamine bishydrochloride derived from variously multiply (13)C- and (2)H-labeled samples of glycerol and glyceraldehyde, established by NMR spectroscopy, indicate that the three-carbon unit C-6,5,5' of pyridoxamine is derived intact from a triose.
Zeidler
Johannes
J
Department of Chemistry, McMaster University, Hamilton, Ontario, Canada L8S 4M1.
Gupta
Ram Nath
RN
Sayer
Brian G
BG
Spenser
Ian D
ID
D002175:Candida / Q000378:metabolism*; D002247:Carbon Isotopes; D003903:Deuterium; D004926:Escherichia coli / Q000378:metabolism; D005985:Glyceraldehyde; D005990:Glycerol; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D011733:Pyridoxamine / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D014306:Trioses / Q000378:metabolism; D025101:Vitamin B 6 / Q000096:biosynthesis*
D002247:Carbon Isotopes; D014306:Trioses; D005985:Glyceraldehyde; D011733:Pyridoxamine; D025101:Vitamin B 6; D003903:Deuterium; D005990:Glycerol