title: Muscle fibrillin deficiency in Marfan's syndrome myopathy.
abstract: OBJECTIVE
  To report a family with Marfan's syndrome in whom a myopathy was associated with respiratory failure; muscle biopsies from affected individuals were examined to determine whether there were abnormalities in fibrillin.
  
  
  METHODS
  21 family members underwent detailed clinical examination, including neurological and pulmonary assessment. Muscle biopsies in the most severely affected cases were immunostained using monoclonal antibodies to specific fibrillin components. Genomic DNA from all 21 members was analysed for mutations in the fibrillin gene, FBN1, on 15q21.
  
  
  RESULTS
  13 individuals had a C4621T base change in exon 37 of the FBN1 gene, which in four cases segregated with muscle weakness or evidence of respiratory muscle dysfunction or both. Their muscle biopsies revealed an abnormality in fibrillin immunoreactivity.
  
  
  CONCLUSIONS
  Abnormalities in fibrillin can be detected in muscle biopsies from patients with Marfan's syndrome who have myopathy. This pedigree, with a point mutation in FBN1, also draws attention to the potential for respiratory failure associated with myopathy.
authors.affiliation: Department of Pathology, Glasgow University, Glasgow, UK. wbm1q@clinmed.gla.ac.uk
authors.forename: W M H
authors.initials: WM
authors.lastname: Behan
authors.forename: C
authors.initials: C
authors.lastname: Longman
authors.forename: R K H
authors.initials: RK
authors.lastname: Petty
authors.forename: P
authors.initials: P
authors.lastname: Comeglio
authors.forename: A H
authors.initials: AH
authors.lastname: Child
authors.forename: M
authors.initials: M
authors.lastname: Boxer
authors.forename: P
authors.initials: P
authors.lastname: Foskett
authors.forename: D G F
authors.initials: DG
authors.lastname: Harriman
mesh_terms: D000293:Adolescent; D000328:Adult; D004252:DNA Mutational Analysis; D005260:Female; D000071838:Fibrillin-1; D000071837:Fibrillins; D006801:Humans; D008297:Male; D008382:Marfan Syndrome / Q000150:complications* / Q000235:genetics / Q000473:pathology*; D008840:Microfilament Proteins / Q000032:analysis* / Q000172:deficiency* / Q000235:genetics; D008875:Middle Aged; D009135:Muscular Diseases / Q000150:complications* / Q000235:genetics / Q000473:pathology*; D010375:Pedigree; D012131:Respiratory Insufficiency / Q000209:etiology* / Q000235:genetics / Q000473:pathology*; D012132:Respiratory Muscles / Q000473:pathology; D054392:Adipokines
chemical_list: D000071838:Fibrillin-1; D000071837:Fibrillins; D008840:Microfilament Proteins; C000605722:FBN1 protein, human; D054392:Adipokines