Herpes simplex virus infection of murine sensory ganglia induces proliferation of neuronal satellite cells.
Herpes simplex virus (HSV) is a virtually ubiquitous human pathogen that, following cutaneous infection, latently infects neurons of sensory ganglia. Satellite cells (SCs) ensheath and provide metabolic support for these neurons, and could potentially participate in controlling HSV disease. Although SCs are restrictive for HSV replication, hypercellularity of non-neuronal cells in ganglia is prominent during HSV infection in animal models. SCs proliferate in response to trauma, e.g. nerve cut or crush, but it is not known if proliferation occurs in response to viral infection. To address this issue, cell proliferation, measured by bromodeoxyuridine (BrdU) uptake, and immune infiltrate, measured by CD45 labelling, were examined during acute infection in a mouse model. Because SCs do not express CD45, the BrdU(+) CD45(-) cell subset represents the proliferating SC population. We report that during acute ganglionic HSV infection there is a substantial increase in SC numbers. We suggest that SC proliferation in response to HSV infection may occur in order to facilitate neuronal survival.
Elson
Karen
K
Herpes Research Laboratory, Institute of Medical and Veterinary Science, Frome Road, Adelaide, SA 5000, Australia.
Speck
Peter
P
Herpes Research Laboratory, Institute of Medical and Veterinary Science, Frome Road, Adelaide, SA 5000, Australia.
Simmons
Anthony
A
Department of Pediatrics and Sealy Center for Vaccine Development, 2.330 Children's Hospital, 301 University Boulevard, Galveston, TX 77555-0373, USA.
D000208:Acute Disease; D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002455:Cell Division; D004195:Disease Models, Animal; D005260:Female; D017950:Ganglia, Sensory / Q000166:cytology / Q000821:virology*; D006561:Herpes Simplex / Q000276:immunology / Q000503:physiopathology* / Q000821:virology; D006801:Humans; D017493:Leukocyte Common Antigens / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000166:cytology* / Q000821:virology*; D018139:Simplexvirus / Q000472:pathogenicity* / Q000502:physiology
D017493:Leukocyte Common Antigens; D001973:Bromodeoxyuridine