Apatite-forming ability of carboxyl group-containing polymer gels in a simulated body fluid.
Carboxymethylated chitin, gellan gum, and curdlan gels were soaked in a simulated body fluid (SBF) having ion concentrations nearly equal to those of human blood plasma. Some of the gels had been soaked in a saturated Ca(OH)(2) solution, while others had not. The carboxymethylated chitin and gellan gum gels have carboxyl groups, while the curdlan gel has hydroxyl groups. None of the gels formed apatite on their surfaces in the SBF when they had not been subjected to the Ca(OH)(2) treatment, whereas the carboxymethylated chitin and gellan gum gels formed apatite on their surfaces when they had been subjected to the Ca(OH)(2) treatment. The curdlan gel did not form an apatite deposit even after the Ca(OH)(2) treatment. Apatite formation on the carboxymethylated chitin and gellan gum gels was attributed to the catalytic effect of their carboxyl groups for apatite nucleation, and acceleration of apatite nucleation from released Ca(2+) ions. This result provides a guiding principle for obtaining apatite-organic polymer fiber composites. This composite is expected to have an analogous structure to that of natural bone.
Kawashita
M
M
Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan. kawashita@sung7.kuic.kyoto-u.ac.jp
Nakao
M
M
Minoda
M
M
Kim
H-M
HM
Beppu
T
T
Miyamoto
T
T
Kokubo
T
T
Nakamura
T
T
D001031:Apatites / Q000138:chemical synthesis* / Q000737:chemistry; D032701:Biomimetics / Q000379:methods; D001826:Body Fluids / Q000737:chemistry*; D018786:Bone Substitutes / Q000138:chemical synthesis / Q000737:chemistry*; D002126:Calcium Hydroxide / Q000737:chemistry*; D002245:Carbon Dioxide / Q000737:chemistry; D002686:Chitin / Q000737:chemistry*; D005782:Gels / Q000737:chemistry*; D005936:Glucans / Q000737:chemistry*; D008968:Molecular Conformation; D011108:Polymers / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D047071:beta-Glucans
D001031:Apatites; D018786:Bone Substitutes; D005782:Gels; D005936:Glucans; D011108:Polymers; D011135:Polysaccharides, Bacterial; D047071:beta-Glucans; D002686:Chitin; D002245:Carbon Dioxide; C041069:carboxyl radical; C038459:curdlan; C048288:gellan gum; D002126:Calcium Hydroxide