title: Development of an adhesive sheet for direct counting of bacteria on solid surfaces.
abstract: An adhesive sheet was developed for direct counting of microorganisms on solid surfaces. The sheet consists of a polyurethane film base and water insoluble adhesive. SYBR Green II (for total direct counting) or 6-carboxyfluorescein diacetate (6CFDA) (for fluorescent vital staining) was used for fluorescent microscopy of bacteria collected on the adhesive face of the sheet. Adhesive sheet sampling showed a higher recovery rate for microbial enumeration than conventional swab method or stamp agar. This method is simple, rapid, inexpensive and reproducible.
authors.affiliation: Environmental Science and Microbiology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6, Yamada-oka, Suita, Japan.
authors.forename: Nobuyasu
authors.initials: N
authors.lastname: Yamaguchi
authors.forename: Ai
authors.initials: A
authors.lastname: Ishidoshiro
authors.forename: Yukari
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Takeshi
authors.initials: T
authors.lastname: Saika
authors.forename: Shuji
authors.initials: S
authors.lastname: Senda
authors.forename: Masao
authors.initials: M
authors.lastname: Nasu
mesh_terms: D000269:Adhesives / Q000737:chemistry*; D001431:Bacteriological Techniques / Q000379:methods; D015169:Colony Count, Microbial; D005456:Fluorescent Dyes; D008856:Microscopy, Fluorescence; D011140:Polyurethanes / Q000737:chemistry; D013499:Surface Properties
chemical_list: D000269:Adhesives; D005456:Fluorescent Dyes; D011140:Polyurethanes