A novel method for marking microinjection sites using methylene blue and diaminobenzidine.
A number of different compounds have been microinjected into the brains of animals for the purpose of marking injection sites. In the present study, we describe a novel method for visualising and permanently preserving sites in the brainstem of rats marked by microinjections of methylene blue. Subsequent exposure of the sections to diaminobenzidine (DAB) tetrahydrochloride in the presence of glucose oxidase or infrared light produced an insoluble black precipitate at the injection site. A major advantage of this method is that the injection sites are marked simultaneously with immunohistochemical processing.
Verner
Todd A
TA
Hypertension and Stroke Research Laboratories, Department of Physiology, University of Sydney, Royal North Shore Hospital, St. Leonards, NSW 2065, Australia.
Goodchild
Ann K
AK
Pilowsky
Paul M
PM
D015100:3,3'-Diaminobenzidine / Q000032:analysis*; D000818:Animals; D001933:Brain Stem / Q000737:chemistry; D008297:Male; D008751:Methylene Blue / Q000032:analysis*; D008845:Microinjections / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley
D015100:3,3'-Diaminobenzidine; D008751:Methylene Blue