Characterization of an ethylene receptor homologue from wheat and its expression during leaf senescence.
A wheat ethylene receptor homologue (W-er1) was isolated from a wheat stem cDNA library using the Arabidopsis ETR1 cDNA as a probe. The predicted amino acid sequence of W-er1 is over 70% similar to ERS1 from Arabidopsis and exhibits homology to bacterial two-component response regulators within the histidine kinase domain. Northern hybridization demonstrated that W-er1 was expressed in stem, leaf and root tissues. Treatments known to induce senescence of detached leaves including jasmonate, abscisic acid and wounding, increased the accumulation of W-er1 mRNA, while benzyladenine treatment did not. These data suggest that W-er1 may play a role in the process of leaf senescence.
Ma
Qing-Hu
QH
Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China. mqh@ns.ibcas.ac.cn
Wang
Xing-Mei
XM
D000040:Abscisic Acid / Q000494:pharmacology; D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins; D001593:Benzyl Compounds; D015152:Blotting, Northern; D002734:Chlorophyll / Q000378:metabolism; D003517:Cyclopentanes / Q000494:pharmacology; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D007701:Kinetin; D008969:Molecular Sequence Data; D054883:Oxylipins; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D010940:Plant Proteins / Q000235:genetics*; D011687:Purines; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013314:Stress, Mechanical; D014908:Triticum / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism
D029681:Arabidopsis Proteins; D001593:Benzyl Compounds; D003517:Cyclopentanes; D018076:DNA, Complementary; C095706:ERS protein, Arabidopsis; D054883:Oxylipins; D010940:Plant Proteins; D011687:Purines; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D002734:Chlorophyll; C011006:jasmonic acid; D000040:Abscisic Acid; D000225:Adenine; C480551:benzylaminopurine; D007701:Kinetin