title: A study of age-related IgE pathophysiological changes.
abstract: The literature on immunosenescence has focused mainly on T cell impairment. However, it is well known that B function is also profoundly affected. In particular, several studies have shown age-related changes in immunoglobulin serum levels. Concerning allergic diseases, the incidence of onset of allergic symptoms, as well as their severity, seems to decrease with age. So, the decline of onset of allergic symptoms observed in ageing might result from a decrease of serum total IgE due to an unbalance of cytokines and soluble factors involved in its production. To gain insight into the mechanisms of age related incidence of onset of allergic symptoms, as well as their severity, in this study we have evaluated in a sample of young (12 females and 15 males, range 20-64 years) and old (42 females and 20, males range 70-93 years) individuals serum values of IgE and sCD23 and in vitro Type 2 cytokine production. Total serum IgE levels were quantified by CAP-system fluorescence enzyme immunoassay. Serum CD23 levels were measured by a sandwich enzyme-linked immunoassay. Enzyme immunoassay tests have been used to quantify IL-4, IL-10 and IL-13 on mitogen-stimulated cultures. Serum total IgE and sCD23 in the two groups of young and old subjects were not significantly different. No detectable levels of IL-4, IL-10 and IL-13 were observed in supernatants from unstimulated cultures in all the subjects tested. After 48 h stimulation with PHA, cytokine amounts became detectable in all subjects. However, the values of the cytokines under study were not significantly different between young and old subjects. In our study, we have not been able to show no impairment in the afferent (type 2 cytokine production) and in the central (serum IgE and sCD23 levels) branch of allergic responses. Previous studies have shown that the efferent branch, at least studied as basophil releasability and bronchial responsiveness, is not impaired in elderly. In conclusion, as suggested from the present and previous papers it is questionable whether there is sufficient information to validate the statement that the incidence of allergic diseases decreases with age.
authors.affiliation: Dipartimento di Medicina, Clinica e delle Patologie Emergenti dell'Università degli Studi di Palermo, Via del Vespro 141, 90127, Palermo, Italy. dilo601@unipa.it
authors.forename: Gabriele
authors.initials: G
authors.lastname: Di Lorenzo
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Pacor
authors.forename: Maria
authors.initials: M
authors.lastname: Esposito Pellitteri
authors.forename: Florinda
authors.initials: F
authors.lastname: Listì
authors.forename: Alfredo
authors.initials: A
authors.lastname: Colombo
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Candore
authors.forename: Pasquale
authors.initials: P
authors.lastname: Mansueto
authors.forename: Claudia
authors.initials: C
authors.lastname: Lo Bianco
authors.forename: Vito
authors.initials: V
authors.lastname: Ditta
authors.forename: Giovanni
authors.initials: G
authors.lastname: Battista Rini
authors.forename: Calogero
authors.initials: C
authors.lastname: Caruso
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000276:immunology*; D005260:Female; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology* / Q000503:physiopathology*; D007073:Immunoglobulin E / Q000097:blood*; D016753:Interleukin-10 / Q000378:metabolism; D018793:Interleukin-13 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D017455:Receptors, IgE / Q000097:blood
chemical_list: D018793:Interleukin-13; D017455:Receptors, IgE; D016753:Interleukin-10; D015847:Interleukin-4; D007073:Immunoglobulin E