Altered islet homeostasis before classic insulitis in BB rats.
Young diabetes-prone BioBreeding (BBdp) rats fed a diabetes-promoting, cereal-based, NIH-07 (NIH) diet have decreased islet area compared with rats fed a diabetes-retardant diet at a time when classic insulitis is minimal. This finding raised the possibility that islet homeostasis in BBdp rats may be abnormal. To investigate this possibility further, comparisons were made between BBdp and BB control (BBc) rats fed a diabetes-promoting NIH diet for 22 days after weaning. Pancreatic sections were fixed in Bouin's solution and evaluated using immunohistochemistry and image analysis by staining with antibodies for islet hormones: insulin, glucagon; cell proliferation markers: PCNA, BrdU; markers of islet neogenesis: PDX-1, cytokeratin 20; apoptosis was assessed by morphological changes and TUNEL staining. Body weight of BBdp rats was significantly smaller than BBc rats. Although the total number of islets was higher in BBdp compared with BBc, both islet and beta-cell fraction were similar. BBdp rats had a lower beta-cell mass than BBc rats, although this was not statistically significant. Alpha-cell fraction and beta-cell size were similar. Apoptotic bodies were rare in beta-cells but more frequent in acinar tissue of BBdp rats. When the day-night cycle was reversed to synchronize the apoptotic process, the number of apoptotic bodies in islets and in acinar cells was increased. Apoptotic bodies and BrdU+ or PCNA+ beta-cells were more frequently encountered in islets of BBdp rats. Although the frequency of CK20+ islets in BBdp rats was not different, CK20+ area fraction was smaller in BBdp. The number of extra-islet insulin+ and glucagon+ clusters (<4 cells) was significantly greater in BBdp rats. These data are consistent with an enhanced compensatory or "repair" process in the pancreas of BBdp rats that attempts to maintain islet cell mass by altering homeostasis through increased islet neogenesis.
Wang
G S
GS
The Ottawa Health Research Institute, University of Ottawa, Ottawa, Canada.
Karamchandani
J
J
Pulido
O
O
Rosenberg
L
L
Scott
F W
FW
D000818:Animals; D017209:Apoptosis; D001835:Body Weight; D001973:Bromodeoxyuridine; D002455:Cell Division; D048429:Cell Size; D003922:Diabetes Mellitus, Type 1 / Q000473:pathology / Q000503:physiopathology; D004032:Diet; D002523:Edible Grain; D006706:Homeostasis; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D009929:Organ Size; D010179:Pancreas / Q000473:pathology / Q000502:physiology / Q000503:physiopathology; D010182:Pancreatic Diseases / Q000473:pathology / Q000503:physiopathology*; D051381:Rats; D011913:Rats, Inbred BB
D001973:Bromodeoxyuridine