Molecular genetic identification of Saccharomyces sensu stricto strains from African sorghum beer.
Genetic relationships of 24 phenotypically different strains isolated from sorghum beer in West Africa and the type cultures of the Saccharomyces sensu stricto species were investigated by universally primed polymerase chain reaction (PCR) analysis, microsatellite fingerprinting and PCR-restriction fragment length polymorphism (RFLP) of the ribosomal internal transcribed spacers. The results demonstrate that internal transcribed spacer (ITS) PCR-RFLP analysis with the endonucleases HaeIII, HpaII, ScrFI and TaqI is useful for discriminating S. cerevisiae, S. kudriavzevii, S. mikatae from one another and from the S. bayanus/S. pastorianus and S. cariocanus/S. paradoxus pairs. The sorghum beer strains exhibited the same restriction patterns as the type culture of S. cerevisiae CBS 1171. PCR profiles generated with the microsatellite primer (GTG)(5) and the universal primer N21 were almost identical for all isolates and strain CBS 1171. Despite phenotypic peculiarities, the strains involved in sorghum beer production in Ghana and Burkina Faso belong to S. cerevisiae. However, based on sequencing of the rDNA ITS1 region and Southern hybridisation analysis, these strains represent a divergent population of S. cerevisiae.
Naumova
Elena S
ES
State Institute for Genetics and Selection of Industrial Microorganisms, I-Dorozhnyi 1, Moscow 117545, Russia.
Korshunova
Irina V
IV
Jespersen
Lene
L
Naumov
Gennadi I
GI
D001483:Base Sequence; D001515:Beer / Q000382:microbiology*; D015139:Blotting, Southern; D002050:Burkina Faso; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D014644:Genetic Variation; D005869:Ghana; D018895:Microsatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012340:RNA, Ribosomal, 5.8S / Q000737:chemistry / Q000235:genetics; D012440:Saccharomyces / Q000145:classification* / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D012340:RNA, Ribosomal, 5.8S