Donor age and gender are the strongest predictors of marrow recovery from cadaveric vertebral bodies.
The purpose of this retrospective analysis was to determine whether there were donor factors that were useful for predicting the yield of nucleated cells from marrow derived from cadaveric vertebral bodies. An analysis of 132 donors over a 6-year period was performed. The average number of vertebral bodies procured from each donor was 10.2 +/- 1.6 (range 5-14). The total number of nucleated cells recovered per donor ranged from 24 x 10(9) to 160 x 10(9) with an average recovery of 69 +/- 28 x 10(9) cells. The cell viability of the recovered cells was > 95%. The average age of the donors was 33 +/- 14 years (mean +/- SD; range 12-65) with an average weight of 169 +/- 41 lb (range 82-308 lb). Males comprised 68% of the donor population. The average number of days from admission to death was 1.9 +/- 1.7 with a range of 1-11.4 days and the interval between asystole and procurement averaged 3.1 +/- 2.3 h (range (0.1-14.7 h). The majority of donors died from head trauma due to an intracranial bleed, gunshot wound, or closed head injury. Regression analysis of the data indicated that the total nucleated cell yield tended to decrease with increasing time between hospital admission and death. The data also indicated that in general female donors yielded lower cell numbers independent of age and male donors under 30 years of age yielded the highest number of cells.
Newman
Helen
H
Northwest Tissue Center & Puget Sound Blood Center, 921 Terry Avenue, Seattle, WA 98104, USA.
Reems
Jo Anna
JA
Rigley
Theodore H
TH
Bravo
Daniel
D
Strong
D Michael
DM
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D001827:Body Height / Q000502:physiology; D001835:Body Weight / Q000502:physiology; D001853:Bone Marrow / Q000033:anatomy & histology*; D002102:Cadaver; D002452:Cell Count; D002469:Cell Separation / Q000379:methods*; D002648:Child; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D012189:Retrospective Studies; D012737:Sex Factors; D013131:Spine / Q000166:cytology* / Q000601:surgery; D014019:Tissue Donors; D020858:Tissue and Organ Harvesting / Q000379:methods*