title: Early neutrophil engraftment following autologous BMT provides a functional predictor of long-term hematopoietic reconstitution.
abstract: BACKGROUND
  Previous studies have demonstrated that the number of CD34+ progenitor cells in the stem cell graft is highly predictive of the rapidity of short-term hematopoietic engraftment. The aim of this study was to identify factors that predict long-term hematopoietic reconstitution (LHR) following autologous BMT.
  
  
  METHODS
  To identify predictors of LHR, peripheral blood counts and marrow biopsies were evaluated at 1 year after transplant in 81 patients with B-cell non-Hodgkin's lymphoma (NHL) or chronic lymphocytic leukemia who underwent autologous BMT. Results were correlated with CD34+ cell dose, granulocyte-monocyte colony-forming units (CFU-GM) infused, and time to neutrophil engraftment (TNE).
  
  
  RESULTS
  Total MNC dose, CD34+ cell dose, and CFU-GM infused were significantly associated with TNE (p = 0.011, p < 0.0001, and p = 0.078, respectively). Patients with chronic lymphocytic leukemia were more likely to have received a low CD34+ cell dose than patients with B-cell non-Hodgkin's lymphoma (p = 0.01). Among the four principal predictors, only TNE showed consistent significant correlation with WBC, absolute neutrophil, and platelet count at 1 year after transplant. Logistic regression model showed that TNE was a more sensitive predictor of LHR than either CD34+ cell dose or CFU-GM infused.
  
  
  CONCLUSIONS
  TNE is an in vivo functional measure of LHR and is a more sensitive predictor of LHR at 1 year after BMT than either CD34+ cell dose or CFU-GM infused.
authors.affiliation: Connell O'Reilly Cell Manipulation Core Facility and the Department of Adult Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: Abba
authors.initials: A
authors.lastname: Zubair
authors.forename: David
authors.initials: D
authors.lastname: Zahrieh
authors.forename: Heather
authors.initials: H
authors.lastname: Daley
authors.forename: Daryls
authors.initials: D
authors.lastname: Schott
authors.forename: John G
authors.initials: JG
authors.lastname: Gribben
authors.forename: Arnold
authors.initials: A
authors.lastname: Freedman
authors.forename: Jerome
authors.initials: J
authors.lastname: Ritz
mesh_terms: D000328:Adult; D018952:Antigens, CD34 / Q000378:metabolism; D001854:Bone Marrow Cells / Q000473:pathology*; D016026:Bone Marrow Transplantation; D002455:Cell Division; D005260:Female; D005544:Forecasting; D006412:Hematopoietic Stem Cells / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000473:pathology*; D011184:Postoperative Period; D012044:Regression Analysis; D013997:Time Factors
chemical_list: D018952:Antigens, CD34