title: The Entner-Doudoroff pathway has little effect on Helicobacter pylori colonization of mice.
abstract: Helicobacter pylori mutants deficient in 6-phosphogluconate dehydratase (6PGD) were constructed. Colonization densities were lower and minimum infectious doses were higher for mutant strains than for wild-type strains. In spite of better colonization, however, wild-type strains did not displace the mutant in cocolonization experiments. Loss of 6PGD diminishes the fitness of H. pylori in vivo, but the pathway is nonessential for colonization.
authors.affiliation: Department of Veterinary Biosciences, Ohio State University, Columbus, Ohio 43210, USA.
authors.forename: Amy E
authors.initials: AE
authors.lastname: Wanken
authors.forename: Tyrrell
authors.initials: T
authors.lastname: Conway
authors.forename: Kathryn A
authors.initials: KA
authors.lastname: Eaton
mesh_terms: D000818:Animals; D015169:Colony Count, Microbial; D005753:Gastric Mucosa / Q000382:microbiology*; D005942:Gluconates / Q000378:metabolism*; D006019:Glycolysis; D016480:Helicobacter pylori / Q000378:metabolism* / Q000472:pathogenicity; D006836:Hydro-Lyases / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D005942:Gluconates; C081217:2-keto-3-deoxy-6-phosphogluconate; D006836:Hydro-Lyases; C022362:phosphogluconate dehydratase; C008884:6-phosphogluconic acid