title: Enzymatic profiling system in a small-molecule microarray.
abstract: [reaction: see text] We have developed a microarray-based strategy for detection of three major classes of hydrolytic enzymes on the basis of their catalytic activities. This enables the sensitive detection of proteins not merely by their bindings but rather by their enzymatic activities. This may provide a valuable tool for screening, identification, and characterization of new enzymes in a high-throughput fashion.
authors.affiliation: Department of Chemistry, National University of Singapore, 3, Science Drive 3, Singapore 117543, Republic of Singapore.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhu
authors.forename: Mahesh
authors.initials: M
authors.lastname: Uttamchandani
authors.forename: Dongbo
authors.initials: D
authors.lastname: Li
authors.forename: Marie L
authors.initials: ML
authors.lastname: Lesaicherre
authors.forename: Shao Q
authors.initials: SQ
authors.lastname: Yao
mesh_terms: D000818:Animals; D002417:Cattle; D003374:Coumarins / Q000737:chemistry; D004798:Enzymes / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes / Q000138:chemical synthesis; D040081:Protein Array Analysis / Q000379:methods*; D013050:Spectrometry, Fluorescence
chemical_list: D003374:Coumarins; D004798:Enzymes; D005456:Fluorescent Dyes