Effects of aging on proliferation and E47 transcription factor activity induced by different stimuli in murine splenic B cells.
In the present paper, we have investigated the effects of aging on the expression and function of the E2A-encoded transcription factor E47 in splenic B lymphocytes, unactivated or activated with different stimuli (LPS, anti-CD40, anti-IgM, alone or in combination with IL-4). Results indicate that unstimulated splenic B cells show very low E47 protein levels as well as E47 DNA-binding activity and that, upon B cell activation, E47 expression and DNA-binding activity are strongly induced in young and, to a significantly lesser extent, in old mice. The level of E47 protein expression in stimulated splenic B cells was found significantly higher in young than in old mice, suggesting that DNA-binding activity correlates with protein expression. These results altogether suggest that the reduced expression of the transcriptional regulator E47 could help explain the reduced B cell functions in aging mice.
Frasca
Daniela
D
Department of Microbiology and Immunology, University of Miami School of Medicine, RMSB, #3146A, 1600 N.W. 10th Ave, Miami, FL 33136, USA. dfrasca@med.miami.edu
Nguyen
Diep
D
Riley
Richard L
RL
Blomberg
Bonnie B
BB
D000375:Aging / Q000276:immunology*; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D019013:CD40 Antigens / Q000276:immunology; D002455:Cell Division / Q000276:immunology; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000276:immunology*; D015536:Down-Regulation / Q000276:immunology; D005260:Female; D015870:Gene Expression / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology; D013268:Stimulation, Chemical; D051820:TCF Transcription Factors; D058125:Transcription Factor 7-Like 1 Protein; D014157:Transcription Factors
D019013:CD40 Antigens; D004268:DNA-Binding Proteins; D007075:Immunoglobulin M; D008070:Lipopolysaccharides; D051820:TCF Transcription Factors; C544913:Tcf7l1 protein, mouse; D058125:Transcription Factor 7-Like 1 Protein; D014157:Transcription Factors