Stress regulates the lymphocyte homing receptor CD62L (L-selectin).
Based on a previous study showing that panic disorder patients had increased expression of na ve phenotype lymphocytes (CD45RA+ and CD62L+), increased plasma cortisol, as well as decreased interleukin-2 (IL-2) producion, we hypothesized that changes in the percentage of expression of these lymphocyte surface molecules could be related to the substances released by the hypothalamic-pituitary-adrenal (HPA) axis and possibly associated to panic disorder (cortisol, IL-2, serotonin and epinephrine). In order to study the altered expression, blood mononuclear cells of normal volunteers were stimulated with mitogen, in the presence of dexamethasone, IL-2, serotonin and epinephrin. CD62L is decreased by IL-2 in vitro. Serotonin and epinephrine did not promote changes in the expression of these surface molecules. The results of the ex vivo study are in agreement with a previous clinical study with panic patients. It could be suggested that stress is responsible for certain immunologic dysfunctions and new studies should be conducted.
Manfro
Gisele Gus
GG
Departamento de Bioquímica, Universidade Federal do Rio Grande do Sul, Serviço de Psiquiatria, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil. gmanfro@pportoweb.com.br
Alexandre Netto
Carlos
C
Pollack
Mark
M
Mezzomo
Kelin Maggioni
KM
Preffer
Frederic
F
Kradin
Richard
R
D000704:Analysis of Variance; D000893:Anti-Inflammatory Agents / Q000097:blood / Q000494:pharmacology; D015415:Biomarkers; D016022:Case-Control Studies; D015815:Cell Adhesion Molecules / Q000276:immunology; D002478:Cells, Cultured / Q000187:drug effects; D003907:Dexamethasone / Q000097:blood / Q000494:pharmacology; D004837:Epinephrine / Q000097:blood*; D005786:Gene Expression Regulation; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D019041:L-Selectin / Q000097:blood*; D018655:Lymphocyte Count; D016584:Panic Disorder / Q000097:blood / Q000276:immunology* / Q000523:psychology; D012701:Serotonin / Q000097:blood; D013315:Stress, Psychological / Q000097:blood / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000893:Anti-Inflammatory Agents; D015415:Biomarkers; D015815:Cell Adhesion Molecules; D007376:Interleukin-2; D019041:L-Selectin; D012701:Serotonin; D003907:Dexamethasone; D004837:Epinephrine