8-Methoxypsoralen and long-wave ultraviolet A inhibit the release of proinflammatory mediators and cytokines from human Fc epsilon RI+ cells: an in vitro study.
The in vitro effects of 8-MOP (concentrations of 20, 100 and 500 ng/ml) alone or in combination with UVA on mediator release from human basophils and skin mast cells (HSMC), activated with immunological and non-immunological stimuli, were investigated. With respect to basophils activated with anti-IgE serum, the results of this study show that: (i) 8-MOP alone inhibits histamine, LTC(4), IL-4 and IL-13 release concentration dependently with a maximal effect at 500 ng/ml (a concentration not reached in vivo); and (ii) UVA irradiation (5 J/cm(2)), after 8-MOP incubation, enhances this inhibitory effect on all released mediators, but for IL-4 and IL-13 the percentage inhibition is also significant for the 8-MOP concentrations (20-100 ng/ml) employed in vivo during PUVA treatment. Moreover, histamine release from basophils activated with non-immunological stimuli (FMLP and A23187) is inhibited by 8-MOP, alone or in combination with UVA. With respect to the HSMC activated with anti-IgE serum, the results show that: (i) 8-MOP alone reduces histamine release concentration dependently; and (ii) this inhibitory effect is enhanced by UVA irradiation (5 J/cm(2)). Histamine release from HSMC activated with A23187 is not modified either by 8-MOP alone or by 8-MOP plus UVA.
de Paulis
Amato
A
Division of Clinical Immunology and Allergy, University of Naples Federico II, Via S. Pansini 5, Italy.
Monfrecola
Giuseppe
G
Casula
Lucia
L
Prizio
Emilia
E
Di Gioia
Luisa
L
Carfora
Maria
M
Russo
Irene
I
de Crescenzo
Gennaro
G
Marone
Gianni
G
D016207:Cytokines / Q000378:metabolism*; D006636:Histamine Release; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism; D066298:In Vitro Techniques; D018836:Inflammation Mediators / Q000378:metabolism*; D008730:Methoxsalen / Q000494:pharmacology*; D011863:Radioimmunoassay; D012867:Skin / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D014466:Ultraviolet Rays
D016207:Cytokines; D018836:Inflammation Mediators; D007073:Immunoglobulin E; D008730:Methoxsalen