Chromosome anomalies detected by interphase fluorescence in situ hybridization: correlation with significant biological features of B-cell chronic lymphocytic leukaemia.
Fluorescence in situ hybridization (FISH) was used to detect 6q-, 11q-, +12, 13q-, 17p- and translocations involving 14q32 in interphase nuclei from blood and/or bone marrow from 113 patients with B-cell chronic lymphocytic leukaemia (B-CLL). A total of 87 patients (77%) had a FISH anomaly: 13q- x 1 was most frequent (64%) followed by 13q- x 2 (28%), +12 (25%), 11q- (15%), 17p- (8%) and 6q- (0%). FISH results for blood and bone marrow cells in 38 patients were similar. Purified CD5+/CD19+ cells from blood were studied in eight patients and results indicate that in some patients not all B cells have FISH anomalies. We used a defined set of hierarchical FISH risk categories to compare FISH results by stable versus progressive disease, age, sex, Rai stage, CD38+ expression and IgVH mutational status. Significant differences in FISH risk distributions were associated with Rai stage, disease status and CD38+, but not by age, sex or IgVH mutational status. To look for baseline factors associated with high-risk disease, multivariate analysis of age, sex, Rai stage, CD38+ and disease status versus FISH risk category was performed. Importantly, only CD38+ was significantly associated with high-risk FISH categories (+12, 11q- and 17p-) after adjustment for the effects of other variables.
Dewald
Gordon W
GW
Division of Laboratory Genetics, Mayo Clinic, Rochester, MN 55905, USA. gdewald@mayo.edu
Brockman
Stephanie R
SR
Paternoster
Sarah F
SF
Bone
Nancy D
ND
O'Fallon
Judith R
JR
Allmer
Cristine
C
James
Charles D
CD
Jelinek
Diane F
DF
Tschumper
Renee C
RC
Hanson
Curtis A
CA
Pruthi
Rajiv K
RK
Witzig
Thomas E
TE
Call
Timothy G
TG
Kay
Neil E
NE
D036541:ADP-ribosyl Cyclase / Q000032:analysis; D051997:ADP-ribosyl Cyclase 1; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000032:analysis; D018941:Antigens, CD19 / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology / Q000502:physiology; D001854:Bone Marrow Cells / Q000276:immunology / Q000502:physiology; D018956:CD5 Antigens / Q000032:analysis; D016009:Chi-Square Distribution; D002869:Chromosome Aberrations; D002880:Chromosomes, Human, Pair 11; D002881:Chromosomes, Human, Pair 12; D002886:Chromosomes, Human, Pair 17; D018450:Disease Progression; D005260:Female; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics* / Q000276:immunology; D008297:Male; D008562:Membrane Glycoproteins; D008875:Middle Aged; D015999:Multivariate Analysis; D009154:Mutation; D011379:Prognosis; D018570:Risk Assessment; D018709:Statistics, Nonparametric
D015703:Antigens, CD; D018941:Antigens, CD19; D018956:CD5 Antigens; D007135:Immunoglobulin Variable Region; D008562:Membrane Glycoproteins; D036541:ADP-ribosyl Cyclase; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1