title: No influence of magnetic fields on cell cycle progression using conditions relevant for patients during MRI.
abstract: The purpose of this study was to examine whether exposure to magnetic fields (MFs) relevant for magnetic resonance imaging (MRI) in clinical routine influences cell cycle progression in two tumor cell lines in vitro. HL60 and EA2 cells were exposed to four types of MFs: (i) static MF of 1.5 and 7.05 T, (ii) extremely low frequency magnetic gradient fields (ELFMGFs) with +/- 10 mT/m and 100 Hz, as well as +/- 100 mT/m and 100 Hz, (iii) pulsed high frequency MF in the radiofrequency (RF) range (63.6 MHz, 5.8 microT), and (iv) a combination of (i-iii). Exposure periods ranged from 1 to 24 h. Cell cycle distribution (G(0)/G(1), S, and G(2)/M phases) was analyzed by flow cytometry. Cell cycle analysis did not reveal differences between the exposed and the control cells. As expected, positive controls with irradiated (8 Gy) HL60 and EA2 cells showed a strong G(2)/M arrest. Using conditions that are relevant for patients during MRI, no influence of MFs on cell cycle progression was observed in these cell lines. Care was taken to control secondary parameters of influence, such as vibration by the MR scanner or temperature to avoid false positive results.
authors.affiliation: Institute of Toxicology, University of Mainz, Mainz, Germany. schiffer@mail.uni-mainz.de
authors.forename: Ilka B
authors.initials: IB
authors.lastname: Schiffer
authors.forename: Wolfgang G
authors.initials: WG
authors.lastname: Schreiber
authors.forename: Robert
authors.initials: R
authors.lastname: Graf
authors.forename: Elke M
authors.initials: EM
authors.lastname: Schreiber
authors.forename: Detlev
authors.initials: D
authors.lastname: Jung
authors.forename: Dirk M
authors.initials: DM
authors.lastname: Rose
authors.forename: Manfred
authors.initials: M
authors.lastname: Hehn
authors.forename: Susanne
authors.initials: S
authors.lastname: Gebhard
authors.forename: Jens
authors.initials: J
authors.lastname: Sagemüller
authors.forename: Hans W
authors.initials: HW
authors.lastname: Spiess
authors.forename: Franz
authors.initials: F
authors.lastname: Oesch
authors.forename: Manfred
authors.initials: M
authors.lastname: Thelen
authors.forename: Jan G
authors.initials: JG
authors.lastname: Hengstler
mesh_terms: D002453:Cell Cycle / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D004574:Electromagnetic Fields / Q000009:adverse effects*; D004781:Environmental Exposure / Q000009:adverse effects* / Q000032:analysis; D018922:HL-60 Cells; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000009:adverse effects*; D011829:Radiation Dosage; D011874:Radiometry / Q000379:methods*