Tetranectin binds hepatocyte growth factor and tissue-type plasminogen activator.
In the search for new ligands for the plasminogen kringle 4 binding-protein tetranectin, it has been found by ligand blot analysis and ELISA that tetranectin specifically bound to the plasminogen-like hepatocyte growth factor and tissue-type plasminogen activator. The dissociation constants of these complexes were found to be within the same order of magnitude as the one for the plasminogen-tetranectin complex. The study also revealed that tetranectin did not interact with the kindred proteins: macrophage-stimulating protein, urokinase-type plasminogen activator and prothrombin. In order to examine the function of tetranectin, a kinetic analysis of the tPA-catalysed plasminogen activation was performed. The kinetic parameters of the tetranectin-stimulated enhancement of tPA were comparable to fibrinogen fragments, which are so far the best inducer of tPA-catalysed plasminogen activation. The enhanced activation was suggested to be caused by tetranectin's ability to bind and accumulate tPA in an active conformation.
Westergaard
Uffe B
UB
Protein Chemistry Laboratory, Department of Molecular and Structural Biology, University of Aarhus, Denmark.
Andersen
Mikkel H
MH
Heegaard
Christian W
CW
Fedosov
Sergey N
SN
Petersen
Torben E
TE
D000818:Animals; D001665:Binding Sites; D001798:Blood Proteins / Q000378:metabolism; D002417:Cattle; D004789:Enzyme Activation; D017228:Hepatocyte Growth Factor / Q000378:metabolism*; D006801:Humans; D007457:Iodine Radioisotopes; D007700:Kinetics; D037181:Lectins, C-Type / Q000378:metabolism*; D008024:Ligands; D010958:Plasminogen / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D011516:Prothrombin / Q000378:metabolism; D010959:Tissue Plasminogen Activator / Q000378:metabolism*
D001798:Blood Proteins; D007457:Iodine Radioisotopes; D037181:Lectins, C-Type; D008024:Ligands; C048648:tetranectin; D017228:Hepatocyte Growth Factor; D011516:Prothrombin; D010958:Plasminogen; D010959:Tissue Plasminogen Activator