Ultraviolet B radiation induces upregulation of calcitonin gene-related peptide levels in human Finn chamber skin samples.
One of the neuropeptides that plays a role in UVB-induced immunomodulation is calcitonin gene-related peptide (CGRP), as demonstrated in several animal studies. CGRP can be detected in human skin, but effects of UVB exposure on CGRP levels in human skin are not known. We determined CGRP levels in human Finn chamber skin samples of 15 UVB-irradiated and 10 control volunteers. Filter samples were collected prior to and immediately after a UVB exposure protocol (5 consecutive days, with one personally determined minimal erythema dose (MED(jp)) per day). CGRP levels in filter samples were determined using a commercially available radioimmunoassay kit. CGRP could be detected in the filter samples and volunteers showed statistically significantly increased levels after UVB exposure. In addition, the CGRP levels of UVB-exposed volunteers were positively correlated with the dose of UVB in J/m(2) that they received on 5 consecutive days. In other words, higher UVB doses resulted in higher CGRP levels. In summary, CGRP, a mediator in UVB-induced immunomodulation, could be detected in human Finn chamber skin samples, and was significantly increased after UVB exposure. The CGRP level appeared to depend on the amount of UVB the volunteers received.
Sleijffers
Annemarie
A
Laboratory for Pathology and Immunobiology, National Institute of Public Health and the Environment, PO Box 1, 3720 BA Bilthoven, The Netherlands. annemarie.sleijffers@rivm.nl
Herreilers
Miriam
M
van Loveren
Henk
H
Garssen
Johan
J
D015740:Calcitonin Gene-Related Peptide / Q000502:physiology*; D005260:Female; D006801:Humans; D008297:Male; D011863:Radioimmunoassay; D012867:Skin / Q000378:metabolism / Q000528:radiation effects*; D014466:Ultraviolet Rays; D015854:Up-Regulation / Q000528:radiation effects*
D015740:Calcitonin Gene-Related Peptide