[Effect of tumor necrosis factor-alpha on differentiation of mesencephalic neural stem cells and proliferation of oligodendrocytes in the rat].
To observe the influence of tumor necrosis factor-alpha (TNF-alpha) on differentiation of rat mesencephalic neural stem cells (NSCs), the numbers of neurons, astrocytes and oligodendrocytes generated from NSCs were analyzed after differentiation for 3 days by using immunocytochemistry technique. The results show that: (1) TNF-alpha enhanced the proportions of neurons and oligodendrocytes in progeny of NSCs; and (2) TNF-alpha induced the proliferation of oligodendrocytes derived from NSCs, but the proliferation of astrocytes was not influenced by TNF-alpha. We conclude that the TNF-alpha could influence the application of NSCs.
Lou
Shu-Jie
SJ
Department of Neurobiology, Second Military Medical University, Shanghai, PR China. loushjie@hotmail.com
Gu
Ping
P
Xu
He
H
Xu
Xiao-Hui
XH
Wang
Ming-Wei
MW
He
Cheng
C
Lu
Chang-Lin
CL
D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000166:cytology; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D008636:Mesencephalon / Q000166:cytology*; D058953:Neural Stem Cells / Q000166:cytology*; D009474:Neurons / Q000166:cytology; D009836:Oligodendroglia / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*
D014409:Tumor Necrosis Factor-alpha