Generation of dendritic cells from lentiviral vector-transduced CD34+ cells from HIV+ donors.
Dendritic cells hold promise as adjuvant for immunotherapy for cancer and infectious diseases. We demonstrate that a significant number of cryopreserved peripheral blood CD34(+) cells from HIV-infected subjects can be transduced with a replication-incompetent lentiviral vector expressing HIV antigens. In addition, untransduced and transduced CD34(+) cells from HIV-infected individuals were able to differentiate into dendritic cells with strong T-cell stimulatory capacity. Thus, cryopreserved CD34(+) cells from HIV-infected subjects may prove useful for immunotherapy for HIV/AIDS.
Gruber
A
A
Department of Medicine, University of California, San Diego, La Jolla, California, USA. Andreas_Gruber@dfci.harvard.edu
Chen
I
I
Kuhen
K L
KL
Wheat
J C
JC
Law
P
P
Wong-Staal
F
F
D018952:Antigens, CD34 / Q000378:metabolism*; D002454:Cell Differentiation; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology / Q000821:virology; D005822:Genetic Vectors; D015488:HIV Antigens / Q000235:genetics* / Q000276:immunology; D015658:HIV Infections / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000821:virology; D006801:Humans; D016086:Lentivirus / Q000235:genetics*; D014161:Transduction, Genetic
D018952:Antigens, CD34; D015488:HIV Antigens