title: Heat-shock preconditioning protects fatty livers in genetically obese Zucker rats from microvascular perfusion failure after ischemia reperfusion.
abstract: Reduced tolerance of steatotic livers to ischemic injury is considered to correlate with impaired microcirculation. The aim of this study was to investigate the impact of heat-shock preconditioning (HSPC) on microcirculatory failure after ischemia/reperfusion (I/R) in steatotic livers by means of intra-vital fluorescence microscopy. Obese Zucker rats were used. In the HS group, rats underwent whole-body hyperthermia followed by 60-min partial liver ischemia. In group IR, rats were exposed only to ischemia. Microcirculation parameters (sinusoidal perfusion rate, sinusoidal diameter, leukocyte-endothelial interaction) were significantly better preserved in the HS group than in the IR group. Liver enzymes, oxygenated glutathione/reduced glutathione (GSSG/GSH) ratio, and electron microscopy showed less damage in the HS group. A marked expression of heat shock protein 72 (HSP72) and heme oxygenase (HO-1) was found only in the livers of group HS. HSPC mitigated the I/R injury of steatotic livers by preventing post-ischemic failure of microcirculation. This beneficial effect was found to be associated with the induction of HSP72 and HO-1.
authors.affiliation: Department of Gastroenterological Surgery, Kyoto University Graduate School of Medicine, Kyoto, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Yamagami
authors.forename: Georg
authors.initials: G
authors.lastname: Enders
authors.forename: Rolf Josef
authors.initials: RJ
authors.lastname: Schauer
authors.forename: Rosemarie
authors.initials: R
authors.lastname: Leiderer
authors.forename: Jörg
authors.initials: J
authors.lastname: Hutter
authors.forename: Yuzo
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Yamaoka
authors.forename: Claus
authors.initials: C
authors.lastname: Hammer
authors.forename: Konrad
authors.initials: K
authors.lastname: Messmer
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001794:Blood Pressure; D005234:Fatty Liver / Q000473:pathology / Q000503:physiopathology* / Q000601:surgery; D043384:Glutamyl Aminopeptidase / Q000378:metabolism; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000378:metabolism; D018869:Heat-Shock Response; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism; D051547:Heme Oxygenase-1; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000201:enzymology / Q000473:pathology / Q000648:ultrastructure; D008102:Liver Circulation; D016031:Liver Transplantation; D008297:Male; D008833:Microcirculation; D008854:Microscopy, Electron; D051381:Rats; D011924:Rats, Zucker; D015427:Reperfusion Injury / Q000503:physiopathology / Q000517:prevention & control*; D019172:Transplantation Conditioning / Q000379:methods*
chemical_list: D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D007770:L-Lactate Dehydrogenase; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1; D000410:Alanine Transaminase; D043384:Glutamyl Aminopeptidase