title: Discovery of novel transcription control relationships with gene regulatory networks generated from multiple-disruption full genome expression libraries.
abstract: Gene regulatory networks elucidated from strategic, genome-wide experimental data can aid in the discovery of novel gene function information and expression regulation events from observation of transcriptional regulation among genes of known and unknown biological function. To create a reliable and comprehensive data set for the elucidation of transcription regulation networks, we conducted systematic genome-wide disruption expression experiments of yeast on 118 genes with known involvement in transcription regulation. We report several novel regulatory relationships between known transcription factors and other genes with previously unknown biological function discovered with this expression library. Here we report the downstream regulatory subnetworks for UME6 and MET28. The elucidated network topology among these genes demonstrates MET28's role as a nodal point between genes involved in cell division and those involved in DNA repair mechanisms.
authors.affiliation: Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Sachiyo
authors.initials: S
authors.lastname: Aburatani
authors.forename: Kosuke
authors.initials: K
authors.lastname: Tashiro
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Savoie
authors.forename: Mayumi
authors.initials: M
authors.lastname: Nishizawa
authors.forename: Kaori
authors.initials: K
authors.lastname: Hayashi
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Ito
authors.forename: Shigeru
authors.initials: S
authors.lastname: Muta
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Miki
authors.initials: M
authors.lastname: Ogawa
authors.forename: Akiko
authors.initials: A
authors.lastname: Enomoto
authors.forename: Momoka
authors.initials: M
authors.lastname: Masaki
authors.forename: Shouji
authors.initials: S
authors.lastname: Watanabe
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Maki
authors.forename: Yoriko
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Eguchi
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Sakaki
authors.forename: Satoru
authors.initials: S
authors.lastname: Kuhara
mesh_terms: D000465:Algorithms; D005809:Genes, Regulator; D015698:Genomic Library; D020411:Oligonucleotide Array Sequence Analysis; D014158:Transcription, Genetic