Extraction and characterization of collagen with or without telopeptides from chicken skin.
Poultry by-products are not often processed into high-value products. Rather than being transformed into meal for animal feed, a large quantity of chicken skin could be used to produce collagen, which is valued for its unique functional properties. The purpose of this research project was to extract and characterize collagen from chicken skin. Skins were first ground and then were heated to 40 or 60 degrees C to extract the fat. After mechanical separation, the collagen contained in the resulting solid phase was extracted with pepsin or ethylene diamine. Types I and III collagen were then isolated and characterized by SDS PAGE, antigen labeling, determination of tyrosine residues, and transmission electron microscopy. The total collagen content of the skin was recovered from the solid phase following heat treatment at 40 degrees C. Extraction yields varied with the solubilization process: 38.9% of the collagen content in the solid phase was extracted with pepsin and 25.1% with ethylene diamine. Ratios of type I to type III collagen fractionated using NaCl were 74.4:19.8% with pepsin and 62.4:31.7% with ethylene diamine. Characterization tests further revealed the presence of telopeptides solely on ethylene diamine-solubilized collagen. Chicken skin thus appears to be a good alternative source of high-quality collagen.
Cliche
S
S
Agriculture and Agri-Food Canada, Food Research and Development Centre, 3600 Casavant Boulevard West, Saint-Hyacinthe, Quebec, Canada J2S 8E3.
Amiot
J
J
Avezard
C
C
Gariépy
C
C
D000818:Animals; D005591:Chemical Fractionation; D002645:Chickens; D003094:Collagen / Q000737:chemistry / Q000302:isolation & purification* / Q000648:ultrastructure; D024042:Collagen Type I / Q000737:chemistry / Q000302:isolation & purification / Q000648:ultrastructure; D024061:Collagen Type III / Q000737:chemistry / Q000302:isolation & purification / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D005029:Ethylenediamines; D006358:Hot Temperature; D008854:Microscopy, Electron; D010434:Pepsin A; D012867:Skin / Q000737:chemistry*; D012965:Sodium Chloride; D012995:Solubility; D014443:Tyrosine / Q000032:analysis
D024042:Collagen Type I; D024061:Collagen Type III; D005029:Ethylenediamines; D014443:Tyrosine; D012965:Sodium Chloride; C031234:ethylenediamine; D003094:Collagen; D010434:Pepsin A