title: Metabolically engineered methylotrophic yeast cells and enzymes as sensor biorecognition elements.
abstract: An extended definition of the term metabolic engineering is given and its successful use in the construction of biorecognition elements of sensors is demonstrated. It is shown that genetic and chemical modifications of methylotrophic yeast cells provide directed changes in their physiological responses towards methanol, ethanol and formaldehyde resulting in enhanced selectivity and shorter time response of the corresponding potentiometric and amperometric biosensors.
authors.affiliation: Department of Molecular Genetics and Biotechnology, Institute of Cell Biology, Drahomanov Street 14/16, Lviv 79005, Ukraine. gonchar@biochem.lviv.ua
authors.forename: Mykhailo
authors.initials: M
authors.lastname: Gonchar
authors.forename: Mykola
authors.initials: M
authors.lastname: Maidan
authors.forename: Yaroslav
authors.initials: Y
authors.lastname: Korpan
authors.forename: Volodymyr
authors.initials: V
authors.lastname: Sibirny
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Kotylak
authors.forename: Andrei
authors.initials: A
authors.lastname: Sibirny
mesh_terms: D001698:Biomedical Engineering; D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry; D006863:Hydrogen-Ion Concentration; D008660:Metabolism; D015003:Yeasts / Q000235:genetics / Q000378:metabolism*