Regulation by heavy metals and temperature of the human BAG-3 gene, a modulator of Hsp70 activity.
In HeLa cells the expression of the BAG-3 gene, a member of the BAG family, is regulated by heavy metals and temperature, with kinetics of accumulation of mRNAs similar to Hsp70 and metallothioneins. Western blot assays performed with a polyclonal anti-BAG-3 antibody confirmed that higher levels of the protein were present in the cells following heat and metal exposure. By immunofluorescence techniques and cell fractionation assays we demonstrated that following stress BAG-3 protein concentrated in the rough endoplasmic reticulum and associated with the heavy membrane fraction. The role of BAG-3 protein during apoptosis and cellular stress is discussed.
Pagliuca
Maria Gabriella
MG
Dipartimento di Scienze Farmaceutiche, Università degli Studi di Salerno, Fisciano, Italy.
Lerose
Rosa
R
Cigliano
Stefania
S
Leone
Arturo
A
D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; D002104:Cadmium / Q000494:pharmacology; D002352:Carrier Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D018870:Endoplasmic Reticulum, Rough / Q000737:chemistry; D005786:Gene Expression Regulation / Q000187:drug effects; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006367:HeLa Cells; D006358:Hot Temperature; D006801:Humans; D007700:Kinetics; D019216:Metals, Heavy / Q000494:pharmacology*; D015032:Zinc / Q000494:pharmacology
D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; C414431:BAG3 protein, human; D002352:Carrier Proteins; D018840:HSP70 Heat-Shock Proteins; D019216:Metals, Heavy; D002104:Cadmium; D015032:Zinc