Purification and characterization of complement-activating acidic polysaccharides from the fruits of Capsicum annuum.
Hot water-soluble crude polysaccharide (HCAP-0) that was obtained from the fruits of Capsicum annuum showed potent anti-complementary activity. The activity was unchanged by pronase digestion, but decreased by periodate oxidation. The HCAP-0 was fractionated by DEAE ion-exchange chromatography to give two major fractions, HCAP-II and III. These two fractions were finally purified by gel filtration to give HCAP-IIa, HCAPIIIa1, and IIIa2 fractions that had high anti-complementary activities. The HCAP-IIIa1 and IIIa2 consisted of homogeneous polysaccharides. The anti-complementary activities were unaffected by treatment with polymyxin B, indicating that the modes of complement activation were not due to preexisting lipopolysaccharide. The molecular weight and sugar content of HCAP-IIIa2 had potent anti-complementary activity. The highest yields were 55 kDa and 75.9%, and the molar ratio of galactose (Ara:Gal, 1.0:4.6) was higher than other sugars. The crossed immuno-electrophoresis showed that both classical and alternative pathways were activated by HCAP-IIIa2.
Paik
Soon-Young
SY
Department of Microbiology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Ra
Kyung Soo
KS
Chang
In Seop
IS
Park
Yoon Chang
YC
Park
Hee Sung
HS
Baik
Hyung Suk
HS
Yun
Jong Won
JW
Choi
Jang Won
JW
D001419:Bacteria / Q000737:chemistry; D002212:Capsicum / Q000737:chemistry*; D003167:Complement Activation; D005638:Fruit / Q000737:chemistry; D006801:Humans; D008070:Lipopolysaccharides / Q000737:chemistry; D011112:Polymyxin B / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
D008070:Lipopolysaccharides; D011134:Polysaccharides; D011112:Polymyxin B