Gentamicin used as an adjunct to GTR.
OBJECTIVE
To evaluate in a discriminating "capsule" model whether local application of gentamicin may have an added effect on bone formation produced by Bio-Oss and guide tissue regeneration (GTR).


METHODS
Thirty male 3-month-old Wistar rats were used. After elevation of muscle-periosteal flaps, a rigid hemispherical Teflon capsule, loosely packed with 0.025 g of Bio-Oss impregnated with 2 mg/ml gentamicin sulfate (Garamycin), was placed with its open part facing the lateral bone surface of the mandibular ramus (test) in one side of the jaw. A capsule filled only with Bio-Oss (control) was placed on the contralateral side of the jaw. After healing periods of 1, 2 and 4 months, groups of 10 animals were sacrificed and the specimens were processed for histological examination. The volumes of (1) the space created by the capsule, (2) newly formed bone, (3) Bio-Oss particles, (4) loose connective tissue, and (5) acellular space in the capsule were estimated by a point-counting technique in three to four histological sections of each specimen, taken by uniformly random sampling.


RESULTS
The histological evaluation showed limited but increasing bone fill in the capsules from 1 to 4 months in both the test and control sides. After 4 months, the newly formed bone occupied 11.9% (CV: 0.39) of the space created by the capsules at the test sides versus 13.2% (CV: 0.41) at the control sides. There was no statistical significant difference between test and control specimens at any observation time (p>0.05).


CONCLUSIONS
It is concluded that local application of gentamicin has no added effect on bone formation when combined with Bio-Oss and GTR.
Stavropoulos
A
A
Department of Periodontology and Oral Gerodontology, Royal Dental College Aarhus University, Denmark. stavropoulos@odont.au.dk
Kostopoulos
L
L
Mardas
N
N
Nyengaard
J R
JR
Karring
T
T
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000627:therapeutic use*; D001857:Bone Matrix / Q000737:chemistry / Q000473:pathology / Q000637:transplantation; D018786:Bone Substitutes / Q000737:chemistry / Q000627:therapeutic use; D003238:Connective Tissue / Q000473:pathology; D004195:Disease Models, Animal; D005839:Gentamicins / Q000737:chemistry / Q000627:therapeutic use*; D048091:Guided Tissue Regeneration; D008297:Male; D008334:Mandible / Q000473:pathology / Q000601:surgery; D008903:Minerals / Q000737:chemistry / Q000627:therapeutic use; D010012:Osteogenesis / Q000187:drug effects; D010865:Pilot Projects; D051381:Rats; D017208:Rats, Wistar; D018709:Statistics, Nonparametric; D013997:Time Factors; D014945:Wound Healing
D000900:Anti-Bacterial Agents; C077540:Bio-Oss; D018786:Bone Substitutes; D005839:Gentamicins; D008903:Minerals