title: Isolation of (+)-catechin and (-)-epicatechin from Actinidia arguta as bone marrow cell proliferation promoting compounds.
abstract: The MeOH extract of stems of Actinidia arguta promoted proliferation of cultured bone marrow cells and stimulated formation of myeloid colonies from bone marrow cells. (+)-Catechin ( 1) and (-)-epicatechin ( 2) were isolated as active compounds from the MeOH extract. Compounds 1 and 2 stimulated the cell proliferation in a concentration-dependent manner in the range of 1 to 100 mg/mL. Compounds 1 and 2 also stimulated formation of myeloid colonies and enhanced the effect of interleukin-3 (IL-3) to increase the number of colony forming-units in culture (CFU-c). In an ex vivo experiment using a model mouse of decreasing bone marrow functions, orally administrated 1 (100 mg/kg/day) stimulated IL-3-induced CFU-c formation of the bone marrow cells.
authors.affiliation: Experimental Station for Medicinal Plant Studies, Graduate School of Pharmaceutical Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai, Japan.
authors.forename: Fumihide
authors.initials: F
authors.lastname: Takano
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Tanaka
authors.forename: Eiko
authors.initials: E
authors.lastname: Tsukamoto
authors.forename: Nobuo
authors.initials: N
authors.lastname: Yahagi
authors.forename: Shinji
authors.initials: S
authors.lastname: Fushiya
mesh_terms: D029042:Actinidia; D000284:Administration, Oral; D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects; D002392:Catechin / Q000008:administration & dosage / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D004305:Dose-Response Relationship, Drug; D005260:Female; D006410:Hematopoiesis / Q000187:drug effects*; D007377:Interleukin-3 / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology; D018547:Plant Stems
chemical_list: D007377:Interleukin-3; D010936:Plant Extracts; D002392:Catechin