title: The Stimulating Effect of the beta-Endorphin-Like Peptide Immunorphin on the Human T-Lymphoblastoid Cell Line Jurkat Is Mediated by a Non-Opioid Receptor for beta-Endorphin.
abstract: It was shown that beta-endorphin and the synthetic decapeptide SLTCLVKGFY that corresponds to the amino acid sequence 364-373 of the human IgG heavy chain (referred to as immunorphin) is able to stimulate growth of the human T-lymphoblastoid cell line Jurkat. The antagonist of opioid receptors naloxone did not inhibit the stimulating effect of the peptides. Studies on [(3)H]-immunorphin binding to Jurkat cell receptors have demonstrated that it binds with high affinity to naloxone-insensitive receptors (K(d) = 1.3 nM; n = 5.2 x 10(5)). Unlabeled beta-endorphin and the 6-10 fragment of immunorphin completely inhibited the labeled ligand specific binding to naloxone-insensitive receptors on T lymphocytes (K(i) = 1.4 x 10(-7) and 3.7 x 10(-5) M, respectively). Thus, beta-endorphin and immunorphin share the naloxone-insensitive receptors on human T-lymphoblastoid cell line Jurkat.
authors.affiliation: Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, Moscow Region, Russia.
authors.forename: Svetlana B
authors.initials: SB
authors.lastname: Krasnova
authors.forename: Natalia V
authors.initials: NV
authors.lastname: Malkova
authors.forename: Yuliya A
authors.initials: YA
authors.lastname: Kovalitskaya
authors.forename: Yuri A
authors.initials: YA
authors.lastname: Zolotarev
authors.forename: Tatyana A
authors.initials: TA
authors.lastname: Zargarova
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Kolobov
authors.forename: Elena A
authors.initials: EA
authors.lastname: Kampe-Nemm
authors.forename: Elena V
authors.initials: EV
authors.lastname: Navolotskaya
authors.forename: Valery M
authors.initials: VM
authors.lastname: Lipkin
mesh_terms: D006801:Humans; D019169:Jurkat Cells; D009270:Naloxone / Q000494:pharmacology; D010455:Peptides; D011957:Receptors, Opioid / Q000737:chemistry; D001615:beta-Endorphin / Q000378:metabolism
chemical_list: D010455:Peptides; D011957:Receptors, Opioid; D009270:Naloxone; D001615:beta-Endorphin