Cloning and expression of VB12-independent methionine synthase gene responsive to alkaline stress in rice.
VB12-independent methionine synthase is present in higher plants, and catalyzes the methylation of C-homocysteine to form methionine, which is very important for methylation reactions and syntheses of polyamines and ethylene. Under the alkaline condition, using cDNA-RAPD method, a new VB12-independent methionine synthase gene has been cloned and characterized for the first time in rice in this study. The results exhibited that, the cDNA gene entailed 2740 bp, had single copy in the rice genome and encoded peptide of 765 amino acids, the peptide showed 92% and 83% identity with that from Mesembryanthemum cystallinum (U84889) and Cathararanthus roseus (X83499), respectively. It enhanced the transcription more greatly after sodium carbonate treatment for 12 h and 24 h than that of sodium chloride treatment, and then obviously reduced in 48 h later, suggesting that it is related to this stress tolerance in rice.
Xie
Guo-Sheng
GS
Department of Agronomy, National Key Lab of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China.
Liu
Shen-Kui
SK
Takano
Tetsuo
T
You
Zong-Bin
ZB
Zhang
Duan-Pin
DP
D013766:5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002254:Carbonates / Q000494:pharmacology; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000378:metabolism; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D018745:Genome, Plant; D008969:Molecular Sequence Data; D012275:Oryza / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride / Q000494:pharmacology
D002254:Carbonates; D018076:DNA, Complementary; D018744:DNA, Plant; D012965:Sodium Chloride; C005686:sodium carbonate; D013766:5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase; D004262:DNA Restriction Enzymes