title: Influence of Lewis antigen expression by Helicobacter pylori on bacterial internalization by gastric epithelial cells.
abstract: The role of Helicobacter pylori lipopolysaccharide (LPS) Lewis antigens in infection is still not well known. We investigated the influence of Lewis antigen expression by H. pylori on its internalization by AGS cells and the epithelium of human gastric xenografts in nude mice using isogenic mutants in LPS biosynthetic genes. In vivo, colonization rates were unaffected by the change in H. pylori Lewis antigen expression, whereas the number of viable intracellular bacteria was significantly higher with wild-type H. pylori strains expressing Lewis antigens when compared to the isogenic mutants in both models. H. pylori strains expressing more Lewis X antigens (Le(x)) were internalized at a higher rate than those expressing less Le(x), type II Lewis antigens (Le(a) or Le(b)) alone, or no Lewis antigens. Thus, Lewis antigens appear to be involved in the internalization of H. pylori by the gastric epithelium.
authors.affiliation: Laboratoire de Bactériologie-Virologie, Faculté de Médecine, Université Henri-Poincaré, Vandoeuvre-les-Nancy, France. a/lozniewski@chu-nancy.fr
authors.forename: Alain
authors.initials: A
authors.lastname: Lozniewski
authors.forename: Xavier
authors.initials: X
authors.lastname: Haristoy
authors.forename: David A
authors.initials: DA
authors.lastname: Rasko
authors.forename: Renée
authors.initials: R
authors.lastname: Hatier
authors.forename: François
authors.initials: F
authors.lastname: Plénat
authors.forename: Diane E
authors.initials: DE
authors.lastname: Taylor
authors.forename: Karine
authors.initials: K
authors.lastname: Angioi-Duprez
mesh_terms: D000818:Animals; D005745:Gastric Acidity Determination; D005753:Gastric Mucosa / Q000382:microbiology*; D016480:Helicobacter pylori / Q000276:immunology / Q000472:pathogenicity*; D006801:Humans; D016256:Lewis X Antigen / Q000502:physiology*; D051379:Mice; D014183:Transplantation, Heterologous
chemical_list: D016256:Lewis X Antigen