[Preparation and identification of anti-fumonisin B1 monoclonal antibody].
Spleen cells from Balb/c mice immunized with an artificially KLH-glutaraldehyde fumonisin B1(FB1) conjugate were fused with murine Sp2/0 myeloma cells. One hybridoma cell line secreting monoclonal antibody against FB1 was established after the fusion cells subcloned for 4 cycles. This antibody was designated as FB(1)4G4. The titer of the antibody from ascites was 1:1 x 10(7).
Guo
Y
Y
Institute of Nutrition and Food Hygiene, Chinese Academy of Preventive Medicine, Beijing 100050, China.
Liu
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X
Liu
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis*; D037341:Fumonisins / Q000737:chemistry / Q000276:immunology*; D006825:Hybridomas / Q000378:metabolism; D007074:Immunoglobulin G / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D009183:Mycotoxins / Q000737:chemistry / Q000276:immunology*
D000911:Antibodies, Monoclonal; D037341:Fumonisins; D007074:Immunoglobulin G; D009183:Mycotoxins; C056933:fumonisin B1