EGF amplifies the replacement of parvalbumin-expressing striatal interneurons after ischemia.
EGF promotes proliferation and migration of stem/progenitor cells in the normal adult brain. The effect of epidermal growth factor on neurogenesis in ischemic brain is unknown, however. Here we show that intraventricular administration of EGF and albumin augments 100-fold neuronal replacement in the injured adult mouse striatum after cerebral ischemia. Newly born immature neurons migrate into the ischemic lesion and differentiate into mature parvalbumin-expressing neurons, replacing more than 20% of the interneurons lost by 13 weeks after ischemia and representing 2% of the total BrdU-labeled cells. These data suggest that administration of EGF and albumin could be used to manipulate endogenous neurogenesis in the injured brain and to promote brain self-repair.
Teramoto
Tetsuyuki
T
Neuroscience Center, Radiology Department, Massachusetts General Hospital and Neuroscience Program, Harvard Medical School, Boston, MA 02129, USA.
Qiu
Jianhua
J
Plumier
Jean-Christophe
JC
Moskowitz
Michael A
MA
D000818:Animals; D002545:Brain Ischemia / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003342:Corpus Striatum / Q000187:drug effects*; D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; D004815:Epidermal Growth Factor / Q000494:pharmacology* / Q000627:therapeutic use; D007395:Interneurons / Q000187:drug effects*; D008297:Male; D051379:Mice; D009419:Nerve Tissue Proteins; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D010320:Parvalbumins / Q000032:analysis*; D010641:Phenotype; D010750:Phosphoproteins / Q000032:analysis
D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; D009419:Nerve Tissue Proteins; D010320:Parvalbumins; D010750:Phosphoproteins; D004815:Epidermal Growth Factor