Mechanism of aerobic transformation of carbon tetrachloride by poplar cells.
The biochemical mechanism of carbon tetrachloride transformation by poplar cells was investigated using an axenic poplar cell culture. After one-day incubations of poplar cells under aerobic conditions, about 1.5% of dosed carbon tetrachloride was transformed to carbon dioxide, about 0.001% to chloroform and about 3% of the carbon was bound to insoluble poplar cellular materials. The production of carbon dioxide increased under aerobic conditions while the formation of chloroform and cell binding of carbon tetrachloride-carbon was enhanced under anaerobic conditions. Both carbon dioxide production and cell binding were significantly inhibited by a general inhibitor of cytochrome P-450 activity (carbon monoxide) and by specific P-450 2E1 inhibitors (chlorzoxazone, isoniazid, 4-methylpyrazole and 1-phenylimidazole). However, no inhibitory effects were observed when the cells were incubated in the presence of lignin peroxidase inhibitors (NaVO3 and 3-amino-1,2,4-triazole). These results suggest that an enzyme similar to mammalian cytochrome P450-2E1 is involved in the metabolism of carbon tetrachloride by poplar cells. This study demonstrates an environmental biodegradative process for carbon tetrachloride that operates under aerobic conditions.
Wang
Xiaoping
X
College of Forest Resources, University of Washington, Seattle, WA 98195, USA.
Gordon
Milton P
MP
Strand
Stuart E
SE
D000332:Aerobiosis; D016960:Agrobacterium tumefaciens / Q000378:metabolism; D001673:Biodegradation, Environmental; D001711:Biotransformation; D002251:Carbon Tetrachloride / Q000378:metabolism*; D002478:Cells, Cultured; D019392:Cytochrome P-450 CYP2E1 / Q000378:metabolism; D065691:Cytochrome P-450 CYP2E1 Inhibitors; D065607:Cytochrome P-450 Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D010100:Oxygen / Q000494:pharmacology; D010544:Peroxidases / Q000037:antagonists & inhibitors; D012988:Soil Microbiology; D014197:Trees / Q000378:metabolism*
D065691:Cytochrome P-450 CYP2E1 Inhibitors; D065607:Cytochrome P-450 Enzyme Inhibitors; D004791:Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System; D002251:Carbon Tetrachloride; D010544:Peroxidases; C042858:lignin peroxidase; D019392:Cytochrome P-450 CYP2E1; D010100:Oxygen