Characterization of Paracoccidioides brasiliensis atypical isolates by random amplified polymorphic DNA analysis.
Two atypical Paracoccidioides brasiliensis strains (yeast form at room temperature) have been isolated from chronically infected patients living in Brazil. Different random primers were used to characterize these isolates and compare them to typical strains. The RAPD patterns allowed the differentiation of all the selected isolates. Their genetic distance ranged from 5% to 80% of non-shared bands depending on the strains and the primer used. The RAPD data were used to build a Wagner phenogram, which showed two major branched with more than 56% of genetic distance separating them. No significant difference was observed between the atypical isolates and the others suggesting that specific genes are involved in the dimorphism phenomenon.
Hahn
Rosane Christine
RC
Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Macedo
Andrea Mara
AM
Santos
Niriana Lara
NL
Resende
Juliana Campos de Pinho
JC
Hamdan
Júnia Soares
JS