Detection of nucleotide polymorphisms in feline calicivirus isolates by reverse transcription PCR and a fluorescence resonance energy transfer probe.
A fluorescence resonance energy transfer system has been developed to detect nucleotide polymorphisms in isolates of feline calicivirus (FCV). Isolates with an exact match to the 28 nucleotide probe gave a melting temperature of 75 degrees C while isolates with one, two, three or four mismatches gave melting temperatures of 71, 65, 60 and 57 degrees C, respectively. The technique, which is simple, rapid and accurate, is suitable for typing field isolates of FCV.
Helps
Chris
C
Division of Veterinary Pathology, Infection and Immunity, Department of Clinical Veterinary Science, University of Bristol, Langford House, Langford, Bristol BS40 5DU, UK. c.r.helps@bristol.ac.uk
Harbour
Dave
D
D001483:Base Sequence; D017927:Calicivirus, Feline / Q000235:genetics* / Q000302:isolation & purification; D031541:Fluorescence Resonance Energy Transfer; D008969:Molecular Sequence Data; D009711:Nucleotides / Q000737:chemistry; D011110:Polymorphism, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
D009711:Nucleotides