title: The genome of goose hemorrhagic polyomavirus, a new member of the proposed subgenus Avipolyomavirus.
abstract: The full-length genome of goose hemorrhagic polyomavirus (GHPV), the ethiologic agent of hemorrhagic nephritis and enteritis of geese, was cloned and sequenced. Transfection of the circular ds DNA with a size of 5256 bp and an organisation typical of polyomaviruses produced viral progeny in cultured goose cells. According to the splicing sites determined by RT-PCR, five open reading frames (ORFs) were found to encode putative proteins with significant similarities to large T antigen and small t antigen as well as VP1, VP2, and VP3 of other polyomaviruses. An additional ORF located in the 5' region of late mRNA, with a coding capacity for 169 amino acids, shows a low degree of homology to VP4 of avian polyomavirus (APV). The alignment of nucleotide sequences and amino acid sequences revealed a relatively close relationship between GHPV and APV. Therefore, grouping of this new polyomavirus into the proposed subgenus Avipolyomavirus is suggested.
authors.affiliation: Institute for Virology, Faculty of Veterinary Medicine, University of Leipzig, Leipzig, Germany. johne@vetmed.uni-leipzig.de
authors.forename: Reimar
authors.initials: R
authors.lastname: Johne
authors.forename: Hermann
authors.initials: H
authors.lastname: Müller
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D003001:Cloning, Molecular; D005777:Geese / Q000821:virology*; D016679:Genome, Viral; D006470:Hemorrhage / Q000662:veterinary* / Q000821:virology; D008969:Molecular Sequence Data; D009393:Nephritis / Q000662:veterinary* / Q000821:virology; D016366:Open Reading Frames; D011120:Polyomavirus / Q000145:classification / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D036022:Capsid Proteins