Enzymatic profiling system in a small-molecule microarray.
[reaction: see text] We have developed a microarray-based strategy for detection of three major classes of hydrolytic enzymes on the basis of their catalytic activities. This enables the sensitive detection of proteins not merely by their bindings but rather by their enzymatic activities. This may provide a valuable tool for screening, identification, and characterization of new enzymes in a high-throughput fashion.
Zhu
Qing
Q
Department of Chemistry, National University of Singapore, 3, Science Drive 3, Singapore 117543, Republic of Singapore.
Uttamchandani
Mahesh
M
Li
Dongbo
D
Lesaicherre
Marie L
ML
Yao
Shao Q
SQ
D000818:Animals; D002417:Cattle; D003374:Coumarins / Q000737:chemistry; D004798:Enzymes / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes / Q000138:chemical synthesis; D040081:Protein Array Analysis / Q000379:methods*; D013050:Spectrometry, Fluorescence
D003374:Coumarins; D004798:Enzymes; D005456:Fluorescent Dyes