Spatiotemporal distribution of neuronal calcium sensor-1 in the developing rat spinal cord.
The present study revealed the localization of neuronal calcium sensor (NCS)-1 immunoreactivity (IR) in the developing rat spinal cord. The NCS-1 IR first appeared at embryonic day 12 in the peripheral nerves and their somata. Intense NCS-1 IR was expressed in ascending and descending tracts in the white matter during the late prenatal period, which gradually decreased to the faint level during postnatal development. Intense NCS-1 IR was colocalized with growth associated protein (GAP)-43 IR in the marginal zone and with the glutamate-aspartate transporter (GLAST) IR in the radial processes traversing the marginal zone. In the adult rat white matter, radially oriented astrocytes and astrocytes in the glia limitans were double-labeled for NCS-1 and glial fibrillary acidic protein (GFAP), whereas small dots on finger-like dendritic projections were double-labeled for NCS-1 and synaptophysin. In the developing gray matter, the NCS-1 IR appeared at embryonic day 12 and gradually increased in the neuronal somata and neuropil, reaching a plateau after the end of the 4th postnatal week. The small dots in neuropil were colabeled for NCS-1 and GFAP or NCS-1 and synaptophysin in the adult rat gray matter. These results strongly suggest that NCS-1 is involved in axogenesis and synaptogenesis in the developing rat spinal cord. NCS-1 can serve as a Ca(2+)-sensor not only in neurons but also in radial glial cells or even in radially oriented astrocytes in the adult rat spinal cord.
Kawasaki
Takayuki
T
Department of Integrative Brain Science, Graduate School of Medicine, Kyoto University, Yoshida Konoe, Sakyo, Kyoto 606-8501, Japan.
Nishio
Takeshi
T
Kurosawa
Hisashi
H
Roder
John
J
Jeromin
Andreas
A
D027322:Amino Acid Transport System X-AG / Q000032:analysis; D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000737:chemistry* / Q000378:metabolism; D001369:Axons / Q000737:chemistry; D002135:Calcium-Binding Proteins / Q000032:analysis* / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D019922:GAP-43 Protein / Q000032:analysis; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D051580:Neuronal Calcium-Sensor Proteins; D009474:Neurons / Q000737:chemistry* / Q000378:metabolism; D009479:Neuropeptides / Q000032:analysis* / Q000378:metabolism; D019581:Neuropil / Q000737:chemistry; D010525:Peripheral Nerves / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000737:chemistry* / Q000254:growth & development* / Q000378:metabolism; D013569:Synapses / Q000737:chemistry; D016708:Synaptophysin / Q000032:analysis
D027322:Amino Acid Transport System X-AG; D002135:Calcium-Binding Proteins; D019922:GAP-43 Protein; D005904:Glial Fibrillary Acidic Protein; D051580:Neuronal Calcium-Sensor Proteins; D009479:Neuropeptides; D016708:Synaptophysin; C101464:frequenin calcium sensor proteins