Evaluation of a collagen patch as a method of enhancing ventriculotomy healing in Japanese quail (Coturnix coturnix japonica).
OBJECTIVE
To assess the ability of a porcine submucosal collagen patch to enhance ventriculotomy healing in quail.


METHODS
Histologic assessment of wound repair after ventriculotomy. Animal Population-Eighteen adult Japanese quail.


METHODS
All quail had a ventriculotomy through the caudoventral thin muscle. The quail were randomly assigned to 1 of 2 groups: group I (n = 9) had routine closure and group II (n = 9) had routine closure and the application of a porcine submucosal collagen patch over the serosal surface of the ventricular suture line. Three quail from each group underwent necropsy at 7, 14, and 21 days after surgery and healing of the ventriculotomy site was evaluated by microscopy.


RESULTS
Ventricular mucosal epithelium was completely healed at 21 days postoperatively. Time to restoration of the ventricular submucosal integrity was variable in both groups. There was evidence of a gross or microscopic perforation in 4 quail in group II, which was statistically significant (P =.041). Quail with perforations had significantly elevated numbers of ventricular serosal lymphoid aggregates compared with those without perforations (P =.029). There were no other significant temporal differences between group I and group II for mortality or histopathologic grading of wound healing.


CONCLUSIONS
A porcine collagen patch did not enhance ventriculotomy wound healing. Subjectively, the collagen patch might have contributed to perforation by the generation of a lymphocytic xenograft rejection response.


CONCLUSIONS
Ventriculotomy can be safely performed using routine closure methods in healthy birds.
Ferrell
Shannon
S
Veterinary Medical Teaching Hospital, and the Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis 95616, USA.
Werner
Jonathan
J
Kyles
Andrew
A
Lowenstine
Linda
L
Kass
Phillip
P
Tell
Lisa
L
D000818:Animals; D001672:Biocompatible Materials; D001715:Bird Diseases / Q000601:surgery*; D003094:Collagen; D003370:Coturnix; D005260:Female; D008297:Male; D011183:Postoperative Complications; D013272:Stomach Diseases / Q000601:surgery / Q000662:veterinary*; D013278:Stomach, Avian / Q000601:surgery*; D013536:Suture Techniques / Q000662:veterinary*; D016896:Treatment Outcome; D014945:Wound Healing
D001672:Biocompatible Materials; D003094:Collagen