Screen-printed electrodes with electropolymerized Meldola Blue as versatile detectors in biosensors.
Electropolymerization of Meldola Blue was carried out by cyclic voltammetry in the range from -0.6 to +1.4 V vs. Ag/AgCl, thus defining a new immobilization procedure of the phenoxazine mediator on screen-printed graphite electrodes. Evidence of polymer formation was provided by electrochemical and Fourier transform infrared spectroscopy (FTIR) data. Following polymerization, Meldola Blue preserved the ability to catalyze NADH oxidation allowing to achieve a detection limit of 2.5 x 10(-6) mol l(-1) and a sensitivity of 3713 microA l mol(-1) in amperometric determinations at 0 V vs. Ag/AgCl. In addition, the polymeric mediator was found to facilitate the reduction of hydrogen peroxide in the absence of peroxidase. Typical calibration at -0.1 V vs. Ag/AgCl shows a detection limit of 8.5 x 10(-5) mol l(-1), a sensitivity of 494 microA l mol(-1) and a linear range from 2.5 x 10(-4) to 5 x 10(-3) mol l(-1) hydrogen peroxide.
Vasilescu
Alina
A
Monitoring and Quality Control Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Bucharest, 4-12 Regina Elisabeta Blvd., 703461 -1, Bucharest, Romania.
Andreescu
Silvana
S
Bala
Camelia
C
Litescu
Simona Carmen
SC
Noguer
Thierry
T
Marty
Jean Louis
JL
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry; D003067:Coenzymes / Q000737:chemistry; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006861:Hydrogen Peroxide / Q000032:analysis; D009243:NAD / Q000032:analysis* / Q000737:chemistry; D010078:Oxazines / Q000138:chemical synthesis / Q000737:chemistry*; D010781:Photography / Q000379:methods; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D020099:Coated Materials, Biocompatible; D003067:Coenzymes; D010078:Oxazines; D011108:Polymers; D009243:NAD; C005559:Meldola blue; D006861:Hydrogen Peroxide