Arsenic species and leachability in the fronds of the hyperaccumulator Chinese brake (Pteris vittata L.).
Arsenic speciation is important not only for understanding the mechanisms of arsenic accumulation and detoxification by hyperaccumulators, but also for designing disposal options of arsenic-rich biomass. The primary objective of this research was to understand the speciation and leachability of arsenic in the fronds of Chinese brake (Pteris vittata L.), an arsenic hyperaccumulator, with an emphasis on the implications for arsenic-rich biomass disposal. Chinese brake was grown for 18 weeks in a soil spiked with 50 mg As kg(-1) as arsenate (AsO4(3-)), arsenite (AsO3(3-)), dimethylarsinic acid (DMA), or methylarsonic acid (MMA). Plant samples were extracted with methanol/water (1:1) and arsenic speciation was performed using high performance liquid chromatography coupled with atomic fluorescence spectrometry. The impacts of air-drying on arsenic species and leachability in the fronds were examined in the laboratory. After 18 weeks, water-soluble arsenic in soil was mainly present as arsenate with little detectable organic species or arsenite regardless of arsenic species added to the soil. However, arsenic in the fronds was primarily present as inorganic arsenite with an average of 94%. Arsenite re-oxidation occurred in the old fronds and the excised dried tissues. Arsenic species in the fronds were slightly influenced by arsenic forms added to the soil. Air-drying of the fronds resulted in leaching of substantial amounts of arsenic. These findings can be of significance when looking at disposal options of arsenic-rich biomass from the point of view of secondary contamination.
Tu
Cong
C
Soil and Water Science Department, University of Florida, Gainesville, FL 32611-0290, USA.
Ma
Lena Q
LQ
Zhang
Weihua
W
Cai
Yong
Y
Harris
Willie G
WG
D001149:Arsenates / Q000032:analysis; D001152:Arsenicals / Q000032:analysis*; D018053:Arsenites / Q000032:analysis; D001673:Biodegradation, Environmental; D002101:Cacodylic Acid / Q000032:analysis; D004787:Environmental Pollution; D018515:Plant Leaves / Q000737:chemistry*; D032495:Pteris / Q000737:chemistry*; D012989:Soil Pollutants / Q000032:analysis*
D001149:Arsenates; D001152:Arsenicals; D018053:Arsenites; D012989:Soil Pollutants; D002101:Cacodylic Acid; C020300:monomethylarsonic acid; C015001:arsenite; C025657:arsenic acid