Interaction between root lesion nematode Pratylenchus vulnus and two species of Fusarium on growth and development of maple seedlings.
Producing healthy seedlings and distributing them to far areas, is one of the most important factor for developing forests. Because of easy planting, rapid growth and good wood quality, Acer velutinum is the most useful species, among the softwood and hardwood trees. Growth and development of the nurseries were effected by different pests and diseases, the nematodes are one of these agents. They are not actively surveyed in the forest nurseries in Iran. On a survey of maple seedling in the forest nurseries Pratylenchus vulnus, Fusarium solani and F. oxysporum were identified. The interaction between these two fungi and nematode were studied in randomized complete block design with four replications and six treatments. For mass production and multiplication of the nematodes, they were sterilized with Streptomycin Sulfate, they were reared on carrot disc culture. The nematodes were then added to each pot. The results showed a highly significant difference (p = 0.01) between different treatments. In the treatment with nematodes alone growth of seedlings was minimum and population density of nematodes in soil and root tissue was maximum. In this treatments 75% of seedlings were died and seedling average height were 2.25 cm. In the treatments with nematode and fungi together the population of nematodes were decreased and the growth of seedlings were increased, in comparison with nematode alone. F. oxysporum showed more antagonistic effect on nematodes than F. solani. In the nematode + F. oxysporum treatment, 25% of seedlings were died and seedling average height was 12.75 cm. In the nematode + F. solani treatment, 50% of seedlings were died and the seedling average height was 5.5 cm. This is the first report of pathogenecity of Pratylenchus vulnus and its interaction whit fungi on maple seedlings in Iran.
Kheiri
A
A
University of Tehran, Faculty of Agriculture, Iran.
Borhani
A
A
Okhovat
M
M
Pourjam
E
E
D031002:Acer / Q000254:growth & development* / Q000382:microbiology / Q000469:parasitology; D000818:Animals; D005670:Fusarium / Q000254:growth & development* / Q000502:physiology; D009348:Nematoda / Q000254:growth & development* / Q000502:physiology; D010935:Plant Diseases / Q000382:microbiology / Q000469:parasitology; D018517:Plant Roots / Q000254:growth & development* / Q000382:microbiology / Q000469:parasitology; D012988:Soil Microbiology