Ee-CBP, a hevein-type antimicrobial peptide from bark of the spindle tree (Euonymus europaeus L.).
Ee-CBP, a hevein-type antimicrobial peptide was isolated from the bark of the spindle tree (Euonymus europaeus L.). This 4992.5 Da protein exhibited a very strong antifungal activity against five different fytopathogenic fungi that were tested. Concentrations required to inhibit the growth of Botrytis cinerea in agar diffusion assays and microtiterplate assays were 5 micrograms/ml and 1 microgram/ml, respectively. Comparative tests further indicated that Ee-CBP is a more potent antifungal protein than Ac-AMP2, an antimicrobial peptide from seeds of Amaranthus caudatus L. when tested with the same fungus.
Van den Bergh
K P B
KP
Katholieke Universiteit Leuven, Faculty of Agricultural and Applied Biological Sciences, Department of Applied Plant Sciences, Laboratory of Phytopathology and Plant Protection, W. de Croylaan 42, B-3001 Heverlee, Belgium.
Van Damme
E J M
EJ
Peumans
W J
WJ
Coosemans
J
J
D000528:Alternaria / Q000187:drug effects; D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides; D020171:Botrytis / Q000187:drug effects; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D002686:Chitin / Q000378:metabolism; D029756:Euonymus / Q000737:chemistry / Q000235:genetics; D005658:Fungi / Q000187:drug effects*; D005670:Fusarium / Q000187:drug effects; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009491:Neurospora / Q000187:drug effects; D024301:Plant Bark / Q000737:chemistry*; D037121:Plant Lectins / Q000494:pharmacology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid
D000935:Antifungal Agents; D023181:Antimicrobial Cationic Peptides; D002352:Carrier Proteins; C047161:Evonymous europa lectin; D037121:Plant Lectins; D010940:Plant Proteins; C045875:hevein; C074242:AMP2 protein, Amaranthus caudatus; D002686:Chitin