title: Structural studies of biomaterials using double-quantum solid-state NMR spectroscopy.
abstract: Proteins directly control the nucleation and growth of biominerals, but the details of molecular recognition at the protein-biomineral interface remain poorly understood. The elucidation of recognition mechanisms at this interface may provide design principles for advanced materials development in medical and ceramic composites technologies. Here, we describe both the theory and practice of double-quantum solid-state NMR (ssNMR) structure-determination techniques, as they are used to determine the secondary structures of surface-adsorbed peptides and proteins. In particular, we have used ssNMR dipolar techniques to provide the first high-resolution structural and dynamic characterization of a hydrated biomineralization protein, salivary statherin, adsorbed to its biologically relevant hydroxyapatite (HAP) surface. Here, we also review NMR data on peptides designed to adsorb from aqueous solutions onto highly porous hydrophobic surfaces with specific helical secondary structures. The adsorption or covalent attachment of biological macromolecules onto polymer materials to improve their biocompatibility has been pursued using a variety of approaches, but key to understanding their efficacy is the verification of the structure and dynamics of the immobilized biomolecules using double-quantum ssNMR spectroscopy.
authors.affiliation: Department of Chemistry, University of Washington, Seattle, WA 98195, USA. drobny@chem.washington.edu
authors.forename: G P
authors.initials: GP
authors.lastname: Drobny
authors.forename: J R
authors.initials: JR
authors.lastname: Long
authors.forename: T
authors.initials: T
authors.lastname: Karlsson
authors.forename: W
authors.initials: W
authors.lastname: Shaw
authors.forename: J
authors.initials: J
authors.lastname: Popham
authors.forename: N
authors.initials: N
authors.lastname: Oyler
authors.forename: P
authors.initials: P
authors.lastname: Bower
authors.forename: J
authors.initials: J
authors.lastname: Stringer
authors.forename: D
authors.initials: D
authors.lastname: Gregory
authors.forename: M
authors.initials: M
authors.lastname: Mehta
authors.forename: P S
authors.initials: PS
authors.lastname: Stayton
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D011487:Protein Conformation; D012471:Salivary Proteins and Peptides / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; C018149:STATH protein, human; D012471:Salivary Proteins and Peptides; D017886:Durapatite