Evaluation of the delta subunit of bovine adaptor protein complex 3 as a receptor for bovine leukaemia virus.
A candidate gene of the bovine leukaemia virus (BLV) receptor (BLVR) was cloned previously and predicted to encode a transmembrane protein. Subsequent cloning of related genes from other organisms indicated that the candidate gene is related, but unique, to a gene family of the delta subunit of the adaptor protein (AP) complex 3, AP-3. Therefore, bovine cDNAs (boAP3delta) that are highly homologous to the candidate gene were cloned and sequenced. The nucleotide sequences suggested that the boAP3delta cDNA encodes the delta subunit of boAP3 without transmembrane domains. Part of the AP3delta cDNA isolated from the lymph node, spleen and MDBK cells, from which the BLVR candidate cDNA was derived, has almost the same nucleotide sequences as the boAP3delta cDNA. A boAP3delta protein tagged with green fluorescent protein was localized in the cytoplasm and incorporated into AP-3 in bovine cells. Unlike the previous report about the candidate gene, the boAP3delta gene introduced into murine NIH 3T3 cells did not increase the susceptibility of the cells to BLV infection. Many small insertions and deletions of nucleotides could generate the predicted transmembrane and cytoplasmic regions of the BLVR protein from the prototypic boAP3delta gene.
Suzuki
Takako
T
Department of Immunology, National Institute of Animal Health, 3-1-5, Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
Matsubara
Yutaka
Y
Department of Planning and Coordination, National Institute of Animal Health, 3-1-5, Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
Kitani
Hiroshi
H
Department of Molecular Biology and Immunology, National Institute of Agrobiological Sciences, 3-1-5, Kannondai, Tsukuba, Ibaraki 305-8602, Japan.
Ikeda
Hidetoshi
H
Department of Infectious Diseases, National Institute of Animal Health, 3-1-5, Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
D016475:3T3 Cells; D033963:Adaptor Protein Complex 3 / Q000737:chemistry* / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002460:Cell Line; D003001:Cloning, Molecular; D049452:Green Fluorescent Proteins; D006801:Humans; D001909:Leukemia Virus, Bovine / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D011991:Receptors, Virus / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA
D033963:Adaptor Protein Complex 3; D008164:Luminescent Proteins; D011991:Receptors, Virus; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins