Uses of thermoresponsive and RGD/insulin-modified poly(vinyl ether)-based hydrogels in cell cultures.
Thermoresponsive hydrogels were synthesized by radiation copolymerization of ethylene glycol vinyl ether (1) and butyl vinyl ether (2) in the presence of cross-linking agent diethylene glycol divinyl ether. The comonomer ratio (monomer 1/monomer 2) and the cross-linker concentration were kept constant at 60:40 (mole percentage in the monomeric mixture) and 4% (mole basis), respectively. The hydrogels showed a volume-phase transition in the temperature range 10-25 degrees C and their swelling behaviour was reversible. The gels were modified by a cell adhesion factor, the RGD sequence of fibronectin, and a cell growth factor, insulin. However, they lost their thermoresponsive character after modification. The use of the gels in cell culture was investigated without using a proteolytic enzyme or serum. Cell culture studies realized by human skin fibroblasts (HS An1) showed that the cells can attach and proliferate on the surface of a thermoresponsive polymer. 80% of the cultured cells were readily detached from the polymer surface by lowering the incubation temperature from 37 degrees C to 10 degrees C for 30 min. In the studies carried out with RGD or insulin-modified hydrogels in serum-free cultures, higher values of cell proliferation (9 x 10(5) cells/ml) were obtained on the insulin-modified hydrogels, whereas higher values of cell attachment were obtained on the RGD-immobilized surfaces.
Gümüşderelioğlu
Menemşe
M
Hacettepe University, Chemical Engineering Department, 06532, Beytepe, Ankara, Turkey. menemse@hacettepe.edu.tr
Karakeçili
Ayşe Gönen
AG
D001672:Biocompatible Materials; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division; D003432:Cross-Linking Reagents; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D006801:Humans; D020100:Hydrogels; D007328:Insulin; D009842:Oligopeptides; D011095:Polyethylenes; D012867:Skin / Q000166:cytology
D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D020100:Hydrogels; D007328:Insulin; D009842:Oligopeptides; D011095:Polyethylenes; C047981:arginyl-glycyl-aspartic acid