title: A heme-deficient strain of Escherichia coli has a three-base pair deletion in a "hotspot" in hemA.
abstract: The key regulatory step in heme biosynthesis in Escherichia coli is at the level of glutamyl-tRNA reductase (GTR), an enzyme which is encoded by hemA. A strain, HU227, with a spontaneous in-frame mutation in hemA has no GTR activity. The mutation is shown to be a three-base deletion at a "hotspot" in the gene. The amino acid sequence in this region is highly conserved.
authors.affiliation: Department of Biology, City College, City University of New York, New York, NY 10031, USA.
authors.forename: Juncheng
authors.initials: J
authors.lastname: Li
authors.forename: Berthony
authors.initials: B
authors.lastname: Deslouches
authors.forename: Sharon D
authors.initials: SD
authors.lastname: Cosloy
authors.forename: Charlotte Sananes
authors.initials: CS
authors.lastname: Russell
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D017124:Conserved Sequence; D004926:Escherichia coli / Q000201:enzymology*; D006418:Heme / Q000096:biosynthesis; D008969:Molecular Sequence Data; D017384:Sequence Deletion
chemical_list: D006418:Heme; D000445:Aldehyde Oxidoreductases; C067282:glutamyl tRNA reductase