title: Role of protein kinase C in the regulation of acetylcholine release in the central nervous system of spontaneously hypertensive rats.
abstract: Previous studies have shown that the central cholinergic nervous system may be involved in blood pressure control. In the present study, we investigated the role of protein kinase C in the regulation of cholinergic nervous activity in the central nervous system in hypertension. In superfused slices of the striatum obtained from spontaneously hypertensive rats and age-matched Wistar-Kyoto rats, we examined the effects of the protein kinase C inhibitor H-7 on the release of [3H]-acetylcholine in vitro. The stimulation-evoked [3H]-acetylcholine release was not different between spontaneously hypertensive rats and Wistar-Kyoto rats. The protein kinase C inhibitor H-7 reduced the stimulation-evoked [3H]-acetylcholine release to a greater extent in spontaneously hypertensive rats than in Wistar-Kyoto rats. The results of the present study showed that the enhanced calcium-protein kinase C system might actively participate in the regulation of cholinergic nervous activity in the central nervous system in hypertension.
authors.affiliation: Division of Cardiology, Wakayama Medical University, Wakayama, Japan. tsudak@mail.wakayama-med.ac.jp
authors.forename: Kazushi
authors.initials: K
authors.lastname: Tsuda
authors.forename: Seiko
authors.initials: S
authors.lastname: Tsuda
authors.forename: Ichiro
authors.initials: I
authors.lastname: Nishio
mesh_terms: D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002352:Carrier Proteins / Q000494:pharmacology; D002490:Central Nervous System / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism*; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism; D006973:Hypertension / Q000378:metabolism*; D066298:In Vitro Techniques; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000502:physiology*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
chemical_list: D002121:Calcium Channel Blockers; D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; C015482:protein kinase modulator; D011493:Protein Kinase C; D000109:Acetylcholine