title: Direct deletion analysis in two Duchenne muscular dystrophy symptomatic females using polymorphic dinucleotide (CA)n loci within the dystrophin gene.
abstract: Duchenne muscular dystrophy (DMD) is the most common hereditary neuromuscular disease. It is inherited as an X-linked recessive trait in which males show clinical manifestations. In some rare cases, the disease can also be manifested in females. The aim of the present study was to determine the molecular alteration in two cases of nonrelated DMD symptomatic carriers with no previous history of DMD. Multiplex PCR is commonly used to search for deletion in the DMD gene of affected males. This method could not be used in females because the normal X chromosome masks the deletion of the mutated one. Therefore, we used a set of seven highly polymorphic dinucleotide (CA)(n) repeat markers that lie within the human dystrophin gene. The deletions were evidenced by hemizygosity of the loci under study. We localized a deletion in the locus 7A (intron 7) on the maternal X chromosome in one case, and a deletion in the region of introns 49 and 50 on the paternal X chromosome in the other. The use of microsatellite genotyping within the DMD gene enables the detection of the mutant allele in female carriers. It is also a useful method to provide DMD families with more accurate genetic counseling.
authors.affiliation: Catedra de Genetica y Biologia Molecular, Facultad de Famacia y Bioquimica, University of Buenos Aires, and Hospital de Clinicas Jose de San Martin, Buenos Aires, Argentina. fgilibert@ffyb.uba.ar
authors.forename: Florencia
authors.initials: F
authors.lastname: Giliberto
authors.forename: Verónica
authors.initials: V
authors.lastname: Ferreiro
authors.forename: Viviana
authors.initials: V
authors.lastname: Dalamón
authors.forename: Ezequiel
authors.initials: E
authors.lastname: Surace
authors.forename: Javier
authors.initials: J
authors.lastname: Cotignola
authors.forename: Sebastián
authors.initials: S
authors.lastname: Esperante
authors.forename: Daniel
authors.initials: D
authors.lastname: Borelina
authors.forename: Sergio
authors.initials: S
authors.lastname: Baranzini
authors.forename: Irene
authors.initials: I
authors.lastname: Szijan
mesh_terms: D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D004247:DNA / Q000737:chemistry; D004252:DNA Mutational Analysis / Q000379:methods*; D017931:DNA Primers / Q000737:chemistry; D018900:Dinucleotide Repeats; D016189:Dystrophin / Q000235:genetics*; D005260:Female; D017353:Gene Deletion; D006580:Genetic Carrier Screening; D006801:Humans; D008969:Molecular Sequence Data; D020388:Muscular Dystrophy, Duchenne / Q000175:diagnosis / Q000235:genetics*; D010375:Pedigree; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D020080:Tandem Repeat Sequences
chemical_list: D017931:DNA Primers; D016189:Dystrophin; D004247:DNA