title: Hydrogel tissue adhesive for sealing corneal incisions.
abstract: The purpose of the study was to assess the efficacy of a newly developed hydrogel tissue adhesive for sealing corneal incisions in ex vivo rabbit eyes. Simple through-and-through central linear corneal incisions measuring 1-5 mm were created in enucleated rabbit eyes. As controls, the leaking pressures were measured immediately after the incisions had been made. Afterwards, the incisions were sealed with hydrogel adhesive in group 1 and with a single 10-0 nylon suture in group 2. The leaking pressure of each incision was measured after the wounds had been sealed in both groups. The leaking pressures of the controls and the sealed incisions were compared for statistical significance using the Wilcoxon signed rank test. The difference between the leaking pressures before and after sealing the incisions in groups 1 and 2 were compared using the Mann-Whitney rank sum test. The leaking pressures were significantly higher after sealing in both groups 1 and 2 compared to the controls for all incision sizes. The differences in the leaking pressures before and after adhesive application were higher than before and after suturing in all incision sizes. The differences were all statistically significant except for the 1-mm incisions. We conclude that the hydrogel glue has the potential to be used for sealing corneal incisions measuring up to 5 mm.
authors.affiliation: Schepens Eye Research Institute, Harvard Medical School, Boston, Mass., USA. defnekalayci@hotmail.com
authors.forename: Defne
authors.initials: D
authors.lastname: Kalayci
authors.forename: Toshio
authors.initials: T
authors.lastname: Fukuchi
authors.forename: Peter G
authors.initials: PG
authors.lastname: Edelman
authors.forename: Amarpreet S
authors.initials: AS
authors.lastname: Sawhney
authors.forename: Mehul C
authors.initials: MC
authors.lastname: Mehta
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Hirose
mesh_terms: D000818:Animals; D003315:Cornea; D065306:Corneal Injuries; D003393:Cranial Sutures; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000627:therapeutic use*; D011817:Rabbits; D019722:Sphygmomanometers; D014014:Tissue Adhesives / Q000627:therapeutic use*; D014945:Wound Healing
chemical_list: D014014:Tissue Adhesives; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate