Dynamic range of fluorescence detection and base-calling accuracy in DNA sequencer based on single-photon counting.
Recently, we developed a family of high-performance automated capillary DNA sequencing instruments based on a single-photon detection of fluorescently labeled DNA fragments. Our machines employ digital and broadband techniques, essential for achieving superior instrument sensitivity and dynamic range. In the present paper, we discuss limitations of the instrument's performance caused by the nonlinearity of single-photon detectors as well as methods for nonlinearity compensation which increase the detection dynamic range and base-calling accuracy.
Gavrilov
Dmitri N
DN
Department of Electrical and Computer Engineering, State University of New York, Stony Brook 11794, USA. gavrilov@ece.sunysb.edu
Gorbovitski
Boris
B
Gouzman
Mikhail
M
Gudkov
Georgiy
G
Stepoukhovitch
Andrei
A
Ruskovoloshin
Victor
V
Tsuprik
Andrei
A
Tyshko
Georgiy
G
Bilenko
Olga
O
Kosobokova
Olga
O
Luryi
Serge
S
Gorfinkel
Vera
V
D004581:Electronics; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000592:standards; D004867:Equipment Design; D005456:Fluorescent Dyes / Q000032:analysis; D009711:Nucleotides / Q000032:analysis*; D017785:Photons; D012016:Reference Values; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA / Q000295:instrumentation* / Q000592:standards
D005456:Fluorescent Dyes; D009711:Nucleotides