title: A structural basis for the difference in specificity between the two jacalin-related lectins from mulberry (Morus nigra) bark.
abstract: The activity and specificity of a galactose-specific and a mannose-specific jacalin-related lectin from the bark of the black mulberry (Morus nigra) tree has been re-investigated using different experimental approaches. Both lectins definitely behave as polyspecific lectins recognizing galactose, mannose, and glucose even though MornigaG and MornigaM interact preferentially with galactose and mannose, respectively. The exceptionally extended size of the carbohydrate-binding site of both lectins apparently accounts for their polyspecific character. Parallel studies with other mannose-specific jacalin-related lectins confirmed that their exclusive specificity towards mannose/glucose relies on a reduced size of their carbohydrate-binding site.
authors.affiliation: Signaux et Messages Cellulaires chez les Végétaux, UMR-CNRS 5546, Pôle de Biotechnologie végétale, Chemin de Borde-Rouge, 31326, Castanet Tolosan, France. Pierre.Rouge@ipbs.fr <Pierre.Rouge@ipbs.fr>
authors.forename: Pierre
authors.initials: P
authors.lastname: Rougé
authors.forename: Willy J
authors.initials: WJ
authors.lastname: Peumans
authors.forename: Annick
authors.initials: A
authors.lastname: Barre
authors.forename: Els J M
authors.initials: EJ
authors.lastname: Van Damme
mesh_terms: D001665:Binding Sites; D001667:Binding, Competitive; D005690:Galactose / Q000737:chemistry / Q000378:metabolism; D008358:Mannose / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D009005:Monosaccharides / Q000737:chemistry / Q000378:metabolism; D031625:Morus / Q000737:chemistry; D024301:Plant Bark / Q000737:chemistry; D037121:Plant Lectins / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D020349:Surface Plasmon Resonance
chemical_list: D009005:Monosaccharides; D037121:Plant Lectins; C043696:jacalin; D008358:Mannose; D005690:Galactose