Neuropeptide Y-induced contraction and its desensitization through the neuropeptide Y receptor subtype in several rat veins.
Exogenous neuropeptide Y produced marked contractions in rat isolated common jugular, brachial, and caudal veins, while it produced little or no contractions in common carotid, brachial, and caudal arteries. Neuropeptide Y (30 nM) produced larger contractions in these veins than did phenylephrine (1 microM), with maximal contractions through the neuropeptide Y receptor and the alpha1-adrenoceptor, respectively. In contrast, neuropeptide Y (30 nM) produced smaller contractions than did phenylephrine (1 microM) in the arteries. Pre-treatment with neuropeptide Y (30 nM) showed remarkable desensitization to neuropeptide Y (30 nM). This desensitization lasted for 1 h in the caudal vein or for 2 h in the common carotid and brachial veins. The neuropeptide Y-induced contraction and its desensitization probably occurred through the neuropeptide Y receptor subtype Y1, because only the neuropeptide Y receptor subtype Y1 was detected in the smooth muscle by the reverse transcriptase-polymerase chain reaction.
Tsurumaki
Tatsuru
T
Division of Pharmacology, Department of Molecular Genetics and Signal Transduction Research, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
Muraoka
Osamu
O
Yamaguchi
Tsuyoshi
T
Higuchi
Hiroshi
H
D000818:Animals; D005552:Forelimb / Q000098:blood supply; D007601:Jugular Veins / Q000187:drug effects / Q000502:physiology; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000502:physiology; D009478:Neuropeptide Y / Q000494:pharmacology* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D017476:Receptors, Neuropeptide Y / Q000819:agonists* / Q000096:biosynthesis / Q000145:classification / Q000502:physiology*; D013623:Tail / Q000098:blood supply; D014661:Vasoconstriction / Q000187:drug effects* / Q000502:physiology
D009478:Neuropeptide Y; D017476:Receptors, Neuropeptide Y; C097212:neuropeptide Y-Y1 receptor