title: Physiological studies on long-term adaptation to salt stress in the extremely halotolerant yeast Candida versatilis CBS 4019 (syn. C. halophila).
abstract: Candida halophila CBS 4019 (syn. C. versatilis) is an extremely salt-tolerant yeast. It was chosen to study the physiology of long-term resistance to salt stress in cells cultivated at increasing NaCl concentrations up to 4 or 5 M. Growth under stress was slow, severely affected not by salt, but rather by initial external pH. Growing on glucose, glycerol and mannitol were produced. Glycerol is the osmolyte and is transported by H(+)/symport. Transport-driven accumulation was though not affected by salt. The role of mannitol is unknown. Internal pH and intracellular volume were constant during growth at all initial pH/salt combinations. H(+)-ATPase activity was not affected by salt.
authors.affiliation: Department of Biology/Environmental Sciences Research Centre (CCA/B), University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: Magda
authors.initials: M
authors.lastname: Silva-Graça
authors.forename: Cândida
authors.initials: C
authors.lastname: Lucas
mesh_terms: D000222:Adaptation, Physiological; D000251:Adenosine Triphosphatases / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects; D002175:Candida / Q000145:classification / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D005990:Glycerol / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008353:Mannitol / Q000378:metabolism; D009997:Osmotic Pressure; D012965:Sodium Chloride / Q000378:metabolism / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D008353:Mannitol; D012965:Sodium Chloride; D000251:Adenosine Triphosphatases; D005990:Glycerol