No evidence for genotoxic effects from 24 h exposure of human leukocytes to 1.9 GHz radiofrequency fields.
The current study extends our previous investigations of 2-h radiofrequency (RF)-field exposures on genotoxicity in human blood cell cultures by examining the effect of 24-h continuous-wave (CW) and pulsed-wave (PW) 1.9 GHz RF-field exposures on both primary DNA damage and micronucleus induction in human leukocyte cultures. Mean specific absorption rates (SARs) ranged from 0 to 10 W/kg, and the temperature within the cultures was maintained at 37.0 +/- 1.0 degrees C for the duration of the 24-h exposure period. No significant differences in primary DNA damage were observed between the sham-treated controls and any of the CW or PW 1.9 GHz RF-field-exposed cultures when processed immediately after the exposure period by the alkaline comet assay. Similarly, no significant differences were observed in the incidence of micronuclei, incidence of micronucleated binucleated cells, frequency of binucleated cells, or proliferation index between the sham-treated controls and any of the CW or PW 1.9 GHz RF-field-exposed cultures. In conclusion, the current study found no evidence of 1.9 GHz RF-field-induced genotoxicity in human blood cell cultures after a 24-h exposure period.
McNamee
J P
JP
Consumer and Clinical Radiation Protection Bureau, Product Safety Programme, Health Canada, 775 Brookfield Road, Ottawa, Ontario, Canada, K1A 1C1. mcnamee@hc-sc.gc.ca
Bellier
P V
PV
Gajda
G B
GB
Lavallée
B F
BF
Marro
L
L
Lemay
E
E
Thansandote
A
A
D000328:Adult; D002478:Cells, Cultured; D004249:DNA Damage; D006801:Humans; D007962:Leukocytes / Q000528:radiation effects*; D048629:Micronuclei, Chromosome-Defective / Q000528:radiation effects*; D008875:Middle Aged; D011846:Radio Waves