title: Relationship between inactivation of p16 gene and gastric carcinoma.
abstract: OBJECTIVE
  To investigate the relationship between inactivation of p16 gene and gastric carcinoma, and the mechanism of inactivation of p16 gene in gastric carcinogenesis.
  
  
  METHODS
  40 fresh tumor tissue specimens were taken from primary gastric cancer patients. Expression of P16 protein was detected by immunohistochemical method. Deletion and point mutation of p16 gene were analyzed by polymerase chain reaction (PCR) and DNA sequencing, respectively.
  
  
  RESULTS
  The frequency of loss of P16 protein expression in the gastric cancer tissue, adjacent nontumor tissue, and distal normal tissue was 77.5 % (31/40), 55.0 % (22/40), and 17.5 % (7/40), respectively (P<0.005). Homozygous deletion of exon 1 and exon 3 was observed in two and three cases, respectively, giving an overall frequency of homozygous deletion of 12.5 %. All five cases had diffuse type gastric carcinoma. No p16 gene point mutation was detected.
  
  
  CONCLUSIONS
  These findings suggest a close correlation between inactivation of p16 gene and gastric carcinoma. Further investigations are needed to testify the mechanism of inactivation of p16 gene in gastric carcinogenesis.
authors.affiliation: Department of Surgery, the Affiliated Yijishan Hospital, Wannan Medical College, Wuhu 241001, Anhui Province, China. zhaoguohai@163.net
authors.forename: Guo-Hai
authors.initials: GH
authors.lastname: Zhao
authors.forename: Tie-Chen
authors.initials: TC
authors.lastname: Li
authors.forename: Liang-Hui
authors.initials: LH
authors.lastname: Shi
authors.forename: Ya-Bin
authors.initials: YB
authors.lastname: Xia
authors.forename: Lin-Ming
authors.initials: LM
authors.lastname: Lu
authors.forename: Wen-Bin
authors.initials: WB
authors.lastname: Huang
authors.forename: Hui-Lan
authors.initials: HL
authors.lastname: Sun
authors.forename: Yi-Sheng
authors.initials: YS
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D001483:Base Sequence; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000378:metabolism; D004273:DNA, Neoplasm / Q000235:genetics; D005091:Exons; D005260:Female; D015870:Gene Expression; D019942:Genes, p16; D006720:Homozygote; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D017354:Point Mutation; D017384:Sequence Deletion; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16; D004273:DNA, Neoplasm