Gamma interferon gene transfection efficiently inhibits proliferation of neurofibroma cell lines in vitro.
Primarily isolated neurofibroma cell lines and a human dermal fibroblast cell line were transfected with human gamma interferon gene in vitro, and changes in the cell proliferation rates were investigated. The proliferation rates of both cell lines were remarkably suppressed after the gene transfection. In particular, the neurofibroma cell lines almost stopped proliferating five days after gene transfection. The tritium thymidine uptake of the fibroblast cell line was almost abolished three days after gene transfection. The culture media from both of the gene-transfected cell lines contained a measurable gamma interferon concentration as late as five days after transfection, this was detected by an enzyme-linked immunosorbent assay. These data suggest that gamma interferon gene therapy might be a possible treatment for intractable or inoperable neurofibromas in patients with von Recklinghausen's disease in the future.
Nakayama
Juichiro
J
Department of Dermatology, Fukuoka University School of Medicine, Fukuoka Japan.
Tanaka
Toshihiro
T
Arakawa
Fumiko
F
Terao
Hiroshi
H
Shimura
Hideo
H
Ikeda
Seiyo
S
Kuroki
Masahide
M
D001483:Base Sequence; D002455:Cell Division / Q000187:drug effects / Q000235:genetics; D002460:Cell Line; D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000166:cytology; D015316:Genetic Therapy / Q000379:methods; D006801:Humans; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000235:genetics / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009456:Neurofibromatosis 1 / Q000235:genetics* / Q000628:therapy*; D016133:Polymerase Chain Reaction; D014162:Transfection; D014407:Tumor Cells, Cultured
D003470:Culture Media; D007371:Interferon-gamma