A novel cDNA encodes a putative hRALY-like protein, hRALYL.
High throughput cDNA sequencing and 5'-rapid amplification of cDNA ends (5'RACE) isolated two cDNAs that shared the same open reading fragment (ORF). Northern blot analysis with the fetal brain mRNA blots detected two transcripts with the length of 3.2 kb and 2.2 kb respectively. The ORF encodes a 291 residues putative protein that shares great homology with hRALY and hnRNPC. So it was named hRALY like protein, hRALYL. Expression pattern was detected by multiple-issue cDNA (MTC) panel based RT-PCR. It revealed that the transcripts of hRALYL were expressed ubiquitously in human tissues with different intensities. The transcript is highest in brain. Blast analysis found the cDNA corresponding to a contig NT_008292, which revealed the gene containing at least 9 exons and located the gene on human chromosome 8q21.13-8q21.2. hRALYL might be a member of hnRNPC subfamily.
Ji
Chao-Neng
CN
State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200433, People's Republic of China.
Chen
Jin-Zhong
JZ
Xie
Yi
Y
Wang
Shu
S
Qian
Ji
J
Zhao
Enpeng
E
Jin
Wei
W
Wu
Xiao-Zhou
XZ
Xu
Wan-Xiang
WX
Ying
Kang
K
Mao
Yu-Min
YM
D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary; D034482:Heterogeneous-Nuclear Ribonucleoprotein Group C / Q000235:genetics; D034441:Heterogeneous-Nuclear Ribonucleoproteins / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data
D018076:DNA, Complementary; D034482:Heterogeneous-Nuclear Ribonucleoprotein Group C; D034441:Heterogeneous-Nuclear Ribonucleoproteins