Effects of the 2-ethylthiobenzimidazole hydrobromide (bemithyl) on carbohydrate metabolism in cirrhotic rat liver.
The effect of the actoprotector bemithyl (2-ethylthiobenzimidazole hydrobromide) on the content of glycogen and activities of glycogen synthase, glycogen phosphorylase, and glucose-6-phosphatase was studied in the cirrhotic rat liver. The content of glycogen and its fraction was determined by a cytofluorimetric method (Kudryavtseva et al. 1974). It has been shown that in cirrhosis the content of total glycogen in hepatocytes increases about 3 times and the content of its stable fraction increases 7.5 times. The activity of glucose-6-phosphatase fell to a level as low as 25% of normal. Activities of glycogen synthase and glycogen phosphorylase in the cirrhotic liver did not differ from normal. In the cirrhotic liver, bemithyl produced a decrease of the total glycogen content which was associated with a decrease of the glycogen synthase activity and an increase of the glucose-6-phosphatase and glycogen phosphorylase activities. Thus, the results of our studies indicate a favorable effect of bemithyl on the cirrhotic liver.
Kudryavtseva
Margarita V
MV
Laboratory of Cellular Pathology, Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia. cellpath@mail.cytspb.rssi.ru
Bezborodkina
Natalia N
NN
Okovity
Sergey V
SV
Kudryavtsey
Boris N
BN
D000818:Animals; D001562:Benzimidazoles / Q000494:pharmacology*; D005434:Flow Cytometry; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D024981:Glycogen Phosphorylase / Q000378:metabolism; D006006:Glycogen Synthase / Q000037:antagonists & inhibitors; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008106:Liver Cirrhosis, Experimental / Q000201:enzymology / Q000378:metabolism*; D008112:Liver Glycogen / Q000096:biosynthesis*; D008297:Male; D051381:Rats
D001562:Benzimidazoles; D008112:Liver Glycogen; C049825:bemethyl; D024981:Glycogen Phosphorylase; D006006:Glycogen Synthase; D005952:Glucose-6-Phosphatase