The AN69 hemofiltration membrane has a decreasing effect on the intracellular diadenosine pentaphosphate concentration of platelets.
OBJECTIVE
The biocompatibility of hemodialysis membranes has a substantial impact on the mortality of patients with end-stage renal failure. In the present study, the effects of hemodialysis on the intracellular amount of diadenosine pentaphosphate (Ap(5)A), a hydrophilic, anionic substance with a low molecular weight, was investigated.


METHODS
The intracellular Ap(5)A concentrations were measured before and after hemodialysis using either polyacrylonitrile (AN69; n = 10) or polysulfone (n = 23) membranes. Ap(5)A was isolated from platelets using affinity chromatography and reversed-phase chromatography methods.


RESULTS
The Ap(5)A concentrations were quantified by ultraviolet absorption at 254 nm. The Ap(5)A concentrations were significantly higher in platelets from the patients with end-stage renal failure as compared with the 21 healthy control subjects (136 +/- 50 vs. 9 +/- 6 fg/platelet; mean +/- SEM, p < 0.01). Before hemodialysis, the intracellular Ap(5)A concentrations in platelets from 10 patients with end-stage renal failure using an AN69 membrane were not significantly different from those in platelets from 23 patients using a polysulfone membrane (93 +/- 39 vs. 155 +/- 70 fg/platelet). However, after a hemodialysis session, the intracellular Ap(5)A concentrations in platelets from patients with end-stage renal failure using an AN69 membrane were significantly lower as compared with those in platelets before hemodialysis (51 +/- 18 vs. 93 +/- 39 fg/platelet, p < 0.05) as well as compared with those in platelets from patients using a polysulfone membrane (51 +/- 18 vs. 250 +/- 59 fg/platelet, p < 0.05).


CONCLUSIONS
It was found that hemofiltration by using an AN69 membrane has a direct effect on the intracellular amount of Ap(5)A and that changes of intracellular hydrophilic substances are dependent on the hemodialysis membrane used.
Jankowski
Joachim
J
Medizinische Klinik IV, Universitätsklinikum Benjamin Franklin, Freie Universität, Berlin, Germany. Joachim.Jankowski@web.de
Schlüter
Hartmut
H
Henning
Lars
L
van der Giet
Markus
M
Jankowski
Vera
V
Zidek
Walter
W
Tepel
Martin
M
D000180:Acrylic Resins; D001792:Blood Platelets / Q000378:metabolism*; D015226:Dinucleoside Phosphates / Q000378:metabolism*; D005260:Female; D006440:Hemofiltration / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D007676:Kidney Failure, Chronic / Q000628:therapy*; D008297:Male; D008567:Membranes, Artificial; D008875:Middle Aged; D011108:Polymers; D006435:Renal Dialysis / Q000295:instrumentation* / Q000379:methods; D013450:Sulfones
D000180:Acrylic Resins; D015226:Dinucleoside Phosphates; D008567:Membranes, Artificial; D011108:Polymers; D013450:Sulfones; C010504:polyacrylonitrile; C017662:polysulfone P 1700; C012275:P(1),P(5)-di(adenosine-5'-)pentaphosphate