title: Protection of interferon-gamma knockout mice against Listeria monocytogenes challenge following intramuscular immunization with DNA vaccines encoding listeriolysin O.
abstract: In this study we evaluated the efficacy of DNA vaccination of IFN-gamma knockout (GKO) mice against Listeria monocytogenes, as these immunodeficient mice are highly susceptible to infection with low numbers of this intracellular bacterial pathogen. Following intramuscular immunization of BALB/c GKO mice with plasmid DNA constructs encoding recombinant forms of the L. monocytogenes hemolysin, listeriolysin O (LLO), we detected the in vivo induction of a LLO(91-99) peptide-specific, protective immune CTL response equivalent to that observed following similar DNA vaccination of normal BALB/c mice. The observed protection represented greatly enhanced immunity for the GKO host, suggesting that DNA vaccination may provide a useful vaccine alternative for certain immunocompromised host populations.
authors.affiliation: Immunology Research, Veterans Affairs Medical Center, P.O. Box 1034, Portland, OR 97207, USA. barryr@ohsu.edu
authors.forename: Ronald A
authors.initials: RA
authors.lastname: Barry
authors.forename: H G
authors.initials: HG
authors.lastname: Archie Bouwer
authors.forename: Tina R
authors.initials: TR
authors.lastname: Clark
authors.forename: Kenneth A
authors.initials: KA
authors.lastname: Cornell
authors.forename: David J
authors.initials: DJ
authors.lastname: Hinrichs
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology*; D001427:Bacterial Toxins; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000627:therapeutic use*; D005260:Female; D006360:Heat-Shock Proteins / Q000276:immunology*; D006460:Hemolysin Proteins; D007273:Injections, Intramuscular; D007371:Interferon-gamma / Q000172:deficiency* / Q000235:genetics* / Q000276:immunology; D008089:Listeria monocytogenes / Q000276:immunology*; D008088:Listeriosis / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000627:therapeutic use*
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D010446:Peptide Fragments; D019444:Vaccines, DNA; D007371:Interferon-gamma; C038705:hlyA protein, Listeria monocytogenes