title: Mycobacterium shottsii sp. nov., a slowly growing species isolated from Chesapeake Bay striped bass (Morone saxatilis).
abstract: Slowly growing, non-pigmented mycobacteria were isolated from striped bass (Morone saxatilis) during an epizootic of mycobacteriosis in the Chesapeake Bay. Growth characteristics, acid-fastness and results of 16S rRNA gene sequencing were consistent with those of the genus Mycobacterium. A unique profile of biochemical reactions was observed among the 21 isolates. A single cluster of eight peaks identified by analysis of mycolic acids (HPLC) resembled those of reference patterns but differed in peak elution times from profiles of reference species of the Mycobacterium tuberculosis complex. One isolate (M1 75T) was placed within the slowly growing mycobacteria by analysis of aligned 168S rRNA gene sequences and was proximate in phylogeny to Mycobacterium ulcerans and Mycobacterium marinum. However, distinct nucleotide differences were detected in the 16S rRNA gene sequence among M175T, M. ulcerans and M. marinum (99.2% similarity). Isolate M175T could be differentiated from other slowly growing, non-pigmented mycobacteria by its inability to grow at 37 degrees C, production of niacin and urease, absence of nitrate reductase and resistance to isoniazid (1 microg ml(-1)), thiacetazone and thiophene-2-carboxylic hydrazide. Based upon these genetic and phenotypic differences, isolate M175T (=ATCC 700981T =NCTC 13215T) is proposed as the type strain of a novel species, Mycobacterium shottsii sp. nov.
authors.affiliation: Virginia Institute of Marine Science, College of William and Mary, PO Box 1346, Gloucester Point, VA 23062, USA.
authors.forename: Martha W
authors.initials: MW
authors.lastname: Rhodes
authors.affiliation: Virginia Institute of Marine Science, College of William and Mary, PO Box 1346, Gloucester Point, VA 23062, USA.
authors.forename: Howard
authors.initials: H
authors.lastname: Kator
authors.affiliation: Virginia Institute of Marine Science, College of William and Mary, PO Box 1346, Gloucester Point, VA 23062, USA.
authors.forename: Shaban
authors.initials: S
authors.lastname: Kotob
authors.affiliation: United States Department of Agriculture, Beltsville, MD, USA.
authors.forename: Peter
authors.initials: P
authors.lastname: van Berkum
authors.affiliation: Virginia Institute of Marine Science, College of William and Mary, PO Box 1346, Gloucester Point, VA 23062, USA.
authors.forename: Ilsa
authors.initials: I
authors.lastname: Kaattari
authors.affiliation: Virginia Institute of Marine Science, College of William and Mary, PO Box 1346, Gloucester Point, VA 23062, USA.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Vogelbein
authors.affiliation: Tuberculosis/Mycobacteriology Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Frederick
authors.initials: F
authors.lastname: Quinn
authors.affiliation: Tuberculosis/Mycobacteriology Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Margaret M
authors.initials: MM
authors.lastname: Floyd
authors.affiliation: Tuberculosis/Mycobacteriology Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: W Ray
authors.initials: WR
authors.lastname: Butler
authors.affiliation: US Geological Survey, Leetown Science Center, National Fish Health Research Laboratory, Kearneysville, WV, USA.
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Ottinger
mesh_terms: D000818:Animals; D001492:Bass / Q000382:microbiology*; D005393:Fish Diseases / Q000453:epidemiology / Q000382:microbiology*; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D009164:Mycobacterium Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D010641:Phenotype; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000032:analysis* / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S