Identification of a new HLA allele, A*1114, in a Chinese family.
A novel HLA-A allele, A*1114, was initially detected in two generations of a Chinese family by unusual polymerase chain reaction based sequence-specific primers ( PCR-SSP) reaction patterns and ambiguous sequence-based typing (SBT). Molecular cloning and sequencing analysis indicated that this new allele differs from HLA-A*1102 by three nucleotide substitutions in exon 3, 524 A-->G, 526 G-->C, and 527 C-->G, thus changing codon 175 from His to Arg (CAT-->CGT) and codon 176 from Ala to Arg (GCG-->CGG). Segregation analysis showed that the proband inherited his mother's HLA haplotype A*1114, B*5801, DRB1*1405. The serologic equivalent of A*1114 is a split antigen HLA-A11.2. A PCR-SSP method was developed to distinguish A*1114 from other A*11 alleles. No further individuals with A*1114 were found in 5000 Chinese bone marrow donors.
Wu
G-G
GG
Shenzhen Institute of Transfusion Medicine, Shenzhen, China.
Cheng
L-H
LH
Li
Z
Z
Deng
Z-H
ZH
Zou
H-Y
HY
Wei
T-L
TL
Zhou
D
D
Li
D-C
DC
Gao
S-Q
SQ
Zhao
T-M
TM
D000483:Alleles; D001483:Base Sequence; D002681:China; D003001:Cloning, Molecular; D015234:HLA-A Antigens / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D016415:Sequence Alignment
D015234:HLA-A Antigens