title: Purification of bacteriocin AS-48 from an Enterococcus faecium strain and analysis of the gene cluster involved in its production.
abstract: The cyclic bacteriocin AS-48 has previously been shown to be produced by Enterococcus faecalis strains. A bacteriocin has been purified from an E. faecium strain (E. faecium 7C5), and it has been found to possess molecular mass, cyclization and amino acid sequence typical of bacteriocin AS-48. In addition to the structural gene as-48A, the sequence analysis of the AS-48 gene cluster present in E. faecium 7C5 has revealed the presence of several putative coding regions presumably involved in bacteriocin production and immunity. The results of DNA hybridization assays have indicated that the AS-48 gene cluster and the gene pd78 are present on the same plasmid, possibly the pPD1 plasmid, in E. faecium 7C5.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Parma, 43100 Parma, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Folli
authors.forename: Ileana
authors.initials: I
authors.lastname: Ramazzina
authors.forename: Patrizia
authors.initials: P
authors.lastname: Arcidiaco
authors.forename: Monica
authors.initials: M
authors.lastname: Stoppini
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Berni
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001430:Bacteriocins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification*; D016984:Enterococcus faecium / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D010455:Peptides; D010957:Plasmids; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001430:Bacteriocins; D010455:Peptides; C064923:TraF protein, Enterococcus faecalis; C051034:BacA protein, Enterococcus faecalis