title: Recombinant human interleukin-10 inhibits proliferation of vascular smooth muscle cells stimulated by advanced glycation end products and neointima hyperplasia after carotid injury in the rat.
abstract: The purposes of this study was to determine the effects of recombinant human interleukin-10 (rhIL-10) on proliferation of vascular smooth muscle cells (VSMCs) stimulated by advanced glycation end products (AGE) and neointima hyperplasia after rat carotid arterial injury. Rat aortic VSMCs were cultured and treated with rhIL-10 or AGE respectively, and then co-treated with rhIL-10 and AGE. Proliferation of VSMCs was quantified by colormetric assay. Cell cycle analysis was performed by flow cytomertry. Sprague-Dawley rats were treated with recombinant human IL-10 (rhIL-10) for 3 d after carotid arteries injury. The ratio of neointima to media area at the site of arterial injury was measured 28 d after balloon injury. The p44/42 MAPK activity was evaluated by the immunoblotting technique using anti-p44/42 phospho-MAPK antibody. Compared to control, AGE stimulated VSMCs proliferation. rhIL-10 alone had no effect on VSMCs growth. With AGE stimulation, rhIL-10, at dose as low as 10 ng/ml, inhibited VSMCs growth (P<0.05). The cell number in G(0)/G(1) phase of AGE and rhIL-10 co-treatment group was higher than that of AGE treatment alone (P<0.01) by flow cytometry analysis. Compared with the control group of neointima hyperplasia in rats, the ratio of neointima to media area of recombinant human IL-10 group was reduced by 45% (P<0.01). The p44/42 MAPK activity was significantly enhanced by AGE. The AGE effects were opposed by rhIL-10. The anti-inflammatory cytokine rhIL-10 inhibits AGE-induced VSMCs proliferation. Recombinant human IL-10 also inhibited neointima hyperplasia after carotid artery injury in rats. The results suggest the possibility that recombinant human IL-10, as a potential therapeutic approach, prevents neointimal hyperplasia.
authors.affiliation: Department of Biochemistry, School of Life Science, Sun Yat-Sen University, Guangzhou, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Ouyang
authors.forename: Li-Sheng
authors.initials: LS
authors.lastname: Peng
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.forename: Wen-Lie
authors.initials: WL
authors.lastname: Peng
authors.forename: Wen-Yan
authors.initials: WY
authors.lastname: Wu
authors.forename: An-Long
authors.initials: AL
authors.lastname: Xu
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology; D050197:Atherosclerosis / Q000503:physiopathology; D020212:Carotid Artery Injuries / Q000473:pathology* / Q000503:physiopathology; D059168:Carotid Intima-Media Thickness; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D017127:Glycation End Products, Advanced / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D006965:Hyperplasia / Q000517:prevention & control; D016753:Interleukin-10 / Q000494:pharmacology*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000187:drug effects*; D058426:Neointima / Q000188:drug therapy* / Q000517:prevention & control; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology; D017539:Tunica Intima / Q000473:pathology
chemical_list: D017127:Glycation End Products, Advanced; D011994:Recombinant Proteins; D016753:Interleukin-10