Radiation-induced leukocyte adhesion to endothelium in normal pancreas and in pancreatic carcinoma of the rat.
High-dose radiation is known to induce an inflammatory reaction in normal and malignant tissue including leukocyte infiltration. The influence of radiation on leukocyte-endothelium interaction in tumor tissue, which precedes leukocyte migration, has not yet been investigated to our knowledge. In our study, intravital microscopy was used to compare the radiogenic effects on leukocyte-endothelium interaction and leukocyte migration in healthy and malignant pancreatic tissue in vivo. An established model of ductlike pancreatic cancer (DSL6A) of syngeneic Lewis rats was utilized. Irradiation with 15 Gy increased the high-affinity leukocyte-endothelium interaction both in normal and malignant tissue. The low-affinity leukocyte-endothelium interaction was not significantly altered. Radiation-induced tumor cell death 12 days after radiation was significantly higher in tumors with moderate and severe leukocyte infiltration compared to low leukocyte infiltration. Thus, radiation-induced leukocyte infiltration in pancreatic carcinoma correlates with the extent of tumor cell death.
Ryschich
Edward
E
Department of Surgery, University of Heidelberg, Heidelberg, Germany.
Harms
Wolfgang
W
Loeffler
Thorsten
T
Eble
Michael
M
Klar
Ernst
E
Schmidt
Jan
J
D000818:Animals; D002448:Cell Adhesion / Q000528:radiation effects; D004730:Endothelium, Vascular / Q000528:radiation effects*; D007962:Leukocytes / Q000276:immunology / Q000528:radiation effects*; D008297:Male; D009368:Neoplasm Transplantation; D010179:Pancreas / Q000098:blood supply / Q000276:immunology / Q000528:radiation effects*; D010190:Pancreatic Neoplasms / Q000098:blood supply / Q000276:immunology* / Q000473:pathology; D051381:Rats; D011917:Rats, Inbred Lew; D014407:Tumor Cells, Cultured