title: Bordetella bronchiseptica PagP is a Bvg-regulated lipid A palmitoyl transferase that is required for persistent colonization of the mouse respiratory tract.
abstract: Bordetella bronchiseptica lipopolysaccharide (LPS) expression varies depending on growth conditions, regulated by the Bvg system. A B. bronchiseptica pagP homologue was identified that is required for Bvg-mediated modification of the lipid A core region of LPS that occurs on switching from the Bvg- to the Bvg+ phase. Structural analysis demonstrated that the lipid A of a B. bronchiseptica pagP mutant differed from wild-type lipid A by the absence of a palmitate group in secondary acylation at the C3' position. The putative pagP promoter drove the expression of a green fluorescent protein (GFP) reporter gene in a Bvg-regulated fashion. These data suggest that B. bronchiseptica pagP encodes a Bvg-regulated lipid A palmitoyl transferase that mediates modification of the lipid A as part of the overall Bvg-mediated adaptation of this organism to changing environmental conditions. We also show that pagP is not required for the initial colonization of the mouse respiratory tract by B. bronchiseptica, but is required for persistence of the organism within this organ.
authors.affiliation: Centre for Veterinary Science, Department of Clinical Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 0ES, UK. ap243@mole.bio.cam.ac.uk
authors.forename: Andrew
authors.initials: A
authors.lastname: Preston
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Maxim
authors.forename: Elinor
authors.initials: E
authors.lastname: Toland
authors.forename: E Jane
authors.initials: EJ
authors.lastname: Pishko
authors.forename: Eric T
authors.initials: ET
authors.lastname: Harvill
authors.forename: Martine
authors.initials: M
authors.lastname: Caroff
authors.forename: Duncan J
authors.initials: DJ
authors.lastname: Maskell
mesh_terms: D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D001885:Bordetella Infections / Q000378:metabolism; D016950:Bordetella bronchiseptica / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002240:Carbohydrate Sequence; D005260:Female; D017930:Genes, Reporter; D006801:Humans; D008070:Lipopolysaccharides / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012137:Respiratory System / Q000378:metabolism / Q000382:microbiology*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D008070:Lipopolysaccharides; D011993:Recombinant Fusion Proteins; D000217:Acyltransferases