Study of the correlation of secondary structure of beta-amyloid peptide (Abeta40) with the hydrophobic exposure under different conditions.
Abeta is the core protein of extracellular plaque of Alzheimer's disease, and its neurotoxicity is relative to its conformation. In the current work, the effects of various factors, such as pH, ionic strength and lipid membranes, on the secondary structure of Abeta were studied by circular dichroism. In addition, we detected the exposure of hydrophobic sites of Abeta under different conditions using ANS fluorescence. The results showed that the hydrophobic exposure of the protein was correlated with the content of 3betasheet conformation in the phospholipid-containing environment. The beta-sheet content and hydrophobic exposure of Abeta both increased when reacted with pure PC vesicles, while no beta-sheet content and very low hydrophobic exposure were detected after reaction with 30% cholesterol containing PC vesicles. Since beta-sheet conformation is considered as the toxic conformation of Afbeta such correlation may be important for the pathology of AD.
Ji
S R
SR
Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane, Tsinghua University, Beijing, People's Republic of China.
Wu
Y
Y
Sui
S F
SF
D016229:Amyloid beta-Peptides / Q000737:chemistry*; D002784:Cholesterol / Q000737:chemistry; D002942:Circular Dichroism; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D008081:Liposomes / Q000737:chemistry*; D008563:Membrane Lipids / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry*; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D013050:Spectrometry, Fluorescence; D013223:Statistics as Topic; D014867:Water / Q000737:chemistry
D016229:Amyloid beta-Peptides; D008081:Liposomes; D008563:Membrane Lipids; D010743:Phospholipids; D014867:Water; D002784:Cholesterol