title: CD46- and CD150-independent endothelial cell infection with wild-type measles viruses.
abstract: Measles virus (MV) infects endothelial cells of the skin, the brain and other organs during acute or persistent infections. Endothelial cells are supposed to play an important role in virus spread from the blood stream to surrounding tissues. CD46 and CD150 (signalling lymphocytic activation molecule, SLAM) have been described as cellular receptors for certain MV strains. We found that human umbilical vein and brain microvascular endothelial cells (HUVECs and HBMECs) were CD46-positive, but did not express SLAM. Wild-type MV strains, which do not use CD46 as a receptor at the surface of transfected Chinese hamster ovary cells, infected HUVECs and HBMECs to varying extents in a strain-dependent way. This infection was not inhibited by antibodies to CD46. These data suggest the presence of an additional unidentified receptor for MV uptake and spread in human endothelial cells.
authors.affiliation: Institut für Virologie und Immunbiologie, Versbacher Str. 7, 97078 Würzburg, Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: Andres
authors.affiliation: Institut für Virologie und Immunbiologie, Versbacher Str. 7, 97078 Würzburg, Germany.
authors.forename: Karola
authors.initials: K
authors.lastname: Obojes
authors.affiliation: Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
authors.forename: Kwang Sik
authors.initials: KS
authors.lastname: Kim
authors.affiliation: Institut für Virologie und Immunbiologie, Versbacher Str. 7, 97078 Würzburg, Germany.
authors.forename: Volker Ter
authors.initials: VT
authors.lastname: Meulen
authors.affiliation: Institut für Virologie und Immunbiologie, Versbacher Str. 7, 97078 Würzburg, Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Schneider-Schaulies
mesh_terms: D015703:Antigens, CD / Q000378:metabolism*; D001921:Brain / Q000098:blood supply; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology* / Q000821:virology*; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism*; D008459:Measles virus / Q000378:metabolism / Q000472:pathogenicity*; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008833:Microcirculation; D011956:Receptors, Cell Surface; D011991:Receptors, Virus / Q000378:metabolism*; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1; D014471:Umbilical Veins
chemical_list: D015703:Antigens, CD; C497857:CD46 protein, human; D006023:Glycoproteins; D007136:Immunoglobulins; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D011991:Receptors, Virus; C000604059:SLAMF1 protein, human; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1