Preimplantation diagnosis for neurofibromatosis.
Preimplantation genetic diagnosis (PGD) has recently been performed for inherited cancer predisposition determined by p53 tumour suppressor gene mutations, suggesting the usefulness of PGD for late onset disorders with genetic predisposition, including those caused by the germline mutations of other tumour suppressor genes. Here PGD was performed for two couples, one at risk for producing a child with maternally derived neurofibromatosis type I (NF1), and the other with paternally derived neurofibromatosis type II (NF2). The procedure involved a standard IVF protocol, combined with testing of oocytes or embryos prior to their transfer back to the patients. Maternal mutation Trp-->Ter (TGG-->TGA) in exon 29 of the NF1 gene was tested by sequential PCR analysis of the first and second polar bodies, and paternal L141P mutation in exon 4 of the NF2 gene by embryo biopsy at the cleavage stage. In both cases, multiplex nested PCR was applied, involving NF1 and NF2 mutation analysis simultaneously with the 3 and 2 linked markers, respectively. Of 57 oocytes tested in four PGD cycles for NF1 mutation, 26 mutation-free oocytes were detected, from which eight were preselected for transfer, two in each cycle. These produced two clinical pregnancies, one confirmed to be mutation free by chorionic villus sampling but ending in a stillbirth, and the other still ongoing. Of 18 embryos analysed in a cycle performed for NF2 mutation, eight mutation-free embryos were detected, three of which were transferred back to the patient, resulting in a singleton pregnancy and the birth of a mutation-free child. This suggests that PGD is a useful approach for avoiding the birth of children with inherited cancer predisposition, determined by NF1 and NF2 gene mutations.
Verlinsky
Yury
Y
Reproductive Genetics Institute and IVF Illinois, Chicago, USA. rgi@flash.net
Rechitsky
Svetlana
S
Verlinsky
Oleg
O
Chistokhina
Anna
A
Sharapova
Tatyana
T
Masciangelo
Christina
C
Levy
Michael
M
Kaplan
Brian
B
Lederer
Kevin
K
Kuliev
Anver
A
D000328:Adult; D018389:Codon, Nonsense; D005260:Female; D006801:Humans; D008297:Male; D020125:Mutation, Missense; D009456:Neurofibromatosis 1 / Q000175:diagnosis* / Q000235:genetics; D016518:Neurofibromatosis 2 / Q000175:diagnosis* / Q000235:genetics; D019836:Preimplantation Diagnosis; D017422:Sequence Analysis, DNA
D018389:Codon, Nonsense