title: Oxygen requirements for growth and citric acid production of Yarrowia lipolytica.
abstract: During continuous cultivation of Yarrowia lipolytica N 1, oxygen requirements for growth and citric acid synthesis were found to depend on the iron concentration in the medium. A coupled effect of oxygen and iron concentrations on the functioning of the mitochondrial electron transport chain in Y. lipolytica N 1 was established. Based on the results obtained in continuous culture, conditions for citric acid production in a batch culture of Y. lipolytica N 1 were proposed. At relatively low pO(2) value and a high iron concentration, citric acid accumulation was as high as 120 g l(-1); the specific rate of citric acid synthesis reached 120 mg citric acid (g cells h)(-1). The mass yield coefficient was 0.87 and the energy yield coefficient was 0.31.
authors.affiliation: Laboratory of Aerobic Metabolism of Microorganisms, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, p-t Nauki 5, Pushchino, Moscow region 142290, Russia. kamzolova@rambler.ru
authors.forename: Svetlana V
authors.initials: SV
authors.lastname: Kamzolova
authors.forename: Nadezda V
authors.initials: NV
authors.lastname: Shishkanova
authors.forename: Igor G
authors.initials: IG
authors.lastname: Morgunov
authors.forename: Tatyana V
authors.initials: TV
authors.lastname: Finogenova
mesh_terms: D000154:Aconitate Hydratase / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002950:Citrate (si)-Synthase / Q000378:metabolism; D019343:Citric Acid / Q000378:metabolism*; D002952:Citric Acid Cycle / Q000502:physiology; D003580:Cytochromes / Q000378:metabolism; D005285:Fermentation; D007501:Iron / Q000378:metabolism; D007522:Isocitrate Lyase / Q000378:metabolism; D007700:Kinetics; D008292:Malate Synthase / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption / Q000502:physiology; D010313:Partial Pressure; D025062:Yarrowia / Q000254:growth & development / Q000378:metabolism*
chemical_list: D003580:Cytochromes; D019343:Citric Acid; D007501:Iron; D002374:Catalase; D002950:Citrate (si)-Synthase; D008292:Malate Synthase; D007522:Isocitrate Lyase; D000154:Aconitate Hydratase; D010100:Oxygen