title: Genetic alterations in primary cutaneous CD30+ anaplastic large cell lymphoma.
abstract: Primary cutaneous CD30+ anaplastic large cell lymphoma (C-ALCL) represents a distinct clinical subtype of CD30+ anaplastic large cell lymphomas. The etiology and underlying molecular pathogenesis of C-ALCL remain unclear. This study aimed to investigate genetic changes in C-ALCL. Comparative genomic hybridization (CGH) analysis of 23 DNA samples from 15 C-ALCL cases identified chromosome imbalances (CI) in 10 samples from eight cases (43%). The mean number of CI per sample was 2.09 +/- 3.86, with gains (2.00 +/- 3.85) more common than losses (0.09 +/- 0.29). The most frequent CI were gains of 1/1p and 5 (50%) and 6, 7, 8/8p, and 19 (38%). Microarray-based CGH analysis of six DNA samples from five cases with CI revealed genomic imbalances (GI) in all of the cases studied. This included oncogene copy number gains of FGFR1 (8p11) in three cases, and NRAS (1p13.2), MYCN (2p24.1), RAF1 (3p25), CTSB (8p22), FES (15q26.1), and CBFA2 (21q22.3) in two cases. Real-time PCR analysis of nine DNA samples from eight cases with CI and GI detected amplifications of CTSB and RAF1 in seven cases (88%), REL (2p13p12) and JUNB (19p13.2) in six cases (75%), and MYCN and YES1 (18p11.3) in four cases (50%). Immunohistochemical staining of paraffin sections from six cases demonstrated expression of JUNB protein in five cases and BCL2 in three cases. These results reveal a consistent pattern of genetic alterations in C-ALCL and provide the molecular basis for further investigation of this disease.
authors.affiliation: Skin Tumour Unit, St. John's Institute of Dermatology, St. Thomas' Hospital, London, United Kingdom. mxmayo@yahoo.co.uk
authors.forename: Xin
authors.initials: X
authors.lastname: Mao
authors.forename: Guy
authors.initials: G
authors.lastname: Orchard
authors.forename: Debra M
authors.initials: DM
authors.lastname: Lillington
authors.forename: Robin
authors.initials: R
authors.lastname: Russell-Jones
authors.forename: Bryan D
authors.initials: BD
authors.lastname: Young
authors.forename: Sean
authors.initials: S
authors.lastname: Whittaker
mesh_terms: D002869:Chromosome Aberrations / Q000706:statistics & numerical data; D002872:Chromosome Deletion; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D005784:Gene Amplification / Q000235:genetics; D018628:Gene Dosage; D006801:Humans; D016130:Immunophenotyping / Q000379:methods / Q000706:statistics & numerical data; D017730:Ki-1 Antigen / Q000032:analysis / Q000096:biosynthesis*; D017728:Lymphoma, Large-Cell, Anaplastic / Q000175:diagnosis / Q000235:genetics*; D008297:Male; D008677:Metaphase / Q000235:genetics; D009154:Mutation; D009693:Nucleic Acid Hybridization / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods / Q000706:statistics & numerical data; D009857:Oncogenes / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012878:Skin Neoplasms / Q000175:diagnosis / Q000235:genetics*
chemical_list: D004273:DNA, Neoplasm; D017730:Ki-1 Antigen