title: Differential alternative splicing expressions of thymidylate synthase isoforms.
abstract: We identified two novel deletion variants of the thymidylate synthase transcript in gastric cell lines. Sequence analyses indicate that none of these variants results in introduction of a premature stop-codon or a frame shifts. In 39 gastric cancer samples, both the full-length and one-deletion variant messages were detected in cancerous as well as non-cancerous tissues. However, another isoform was found in only seven of 39 cancerous tissues. Our results provide important information to assist more detailed studies on the regulation of thymidylate synthase activity.
authors.affiliation: Center for Molecular Biology and Cytogenetics, SRL, Inc, 5-6-50 Shin-machi Hino, Tokyo 191-0002, Japan. hisatomi@srl.srl-inc.co.jp
authors.forename: Hisashi
authors.initials: H
authors.lastname: Hisatomi
authors.forename: Hiromi
authors.initials: H
authors.lastname: Tanemura
authors.forename: Toshiro
authors.initials: T
authors.lastname: Iizuka
authors.forename: Kenji
authors.initials: K
authors.lastname: Katsumata
authors.forename: Kumi
authors.initials: K
authors.lastname: Nagao
authors.forename: Hitomi
authors.initials: H
authors.lastname: Sumida
authors.forename: Harushi
authors.initials: H
authors.lastname: Udagawa
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Hikiji
mesh_terms: D000368:Aged; D017398:Alternative Splicing; D018388:Codon, Terminator; D005091:Exons; D005260:Female; D016368:Frameshift Mutation; D017353:Gene Deletion; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009336:Necrosis; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D020033:Protein Isoforms; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013274:Stomach Neoplasms; D013940:Thymidylate Synthase / Q000737:chemistry* / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D018388:Codon, Terminator; D020033:Protein Isoforms; D012333:RNA, Messenger; D013940:Thymidylate Synthase