Reusable photonucleases: plasmid scission by a uranyl ion impregnated adenine homopolymer in the presence of visible light and sunlight.
Relaxation of supercoiled plasmid by uranyl ions impregnated on a adenylated polymeric support has been observed in the presence of visible light and sunlight. This insoluble polymer support permits facile reusability of the catalytic system and it failed to cleave lysozyme under the conditions employed for plasmid modification.
Madhavaiah
C
C
Department of Chemistry, Indian Institute of Technology-Kanpur, Kanpur 208016 (UP), India.
Verma
Sandeep
S
D000225:Adenine / Q000737:chemistry*; D002384:Catalysis; D004278:DNA, Superhelical / Q000737:chemistry* / Q000187:drug effects / Q000528:radiation effects; D003851:Deoxyribonucleases / Q000737:chemistry* / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000494:pharmacology; D008027:Light; D009690:Nucleic Acid Conformation; D010782:Photolysis; D010957:Plasmids / Q000737:chemistry* / Q000187:drug effects / Q000528:radiation effects; D011108:Polymers / Q000737:chemistry*; D013472:Sunlight; D014501:Uranium / Q000737:chemistry*
D004278:DNA, Superhelical; D016166:Free Radical Scavengers; D011108:Polymers; D014501:Uranium; D003851:Deoxyribonucleases; D000225:Adenine