Capillary electrophoresis with field-enhanced stacking for rapid and sensitive determination of strychnine and brucine.
A new capillary electrophoresis procedure with field-enhanced stacking concentration for the analysis of strychnine and brucine is established. After optimization of the separation and concentration conditions, the two alkaloids can be separated within 5 min and quantified with high sensitivity (The detection limits were 1.0 ng mL(-1) for strychnine and 1.4 ng mL(-1) for brucine). The method was useful for qualitative and quantitative analysis of strychnine and brucine in Strychnos nux-vomica L with recovery of 105.1% for strychnine and 98.4% for brucine.
Zhang
Jiyou
J
Department of Chemistry, Lanzhou University, 730000 Lanzhou, PR China.
Wang
Shufang
S
Chen
Xingguo
X
Hu
Zhide
Z
Ma
Xiao
X
D004365:Drugs, Chinese Herbal / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D019684:Magnoliopsida / Q000737:chemistry; D012680:Sensitivity and Specificity; D013331:Strychnine / Q000031:analogs & derivatives* / Q000032:analysis* / Q000302:isolation & purification; D031461:Strychnos nux-vomica
D004365:Drugs, Chinese Herbal; C083806:brucine; D013331:Strychnine