Expression of vascular adhesion protein-1 in normal and inflamed mice lungs and normal human lungs.
Recently, vascular adhesion protein-1 (VAP-1) was implicated in adhesion and transmigration of lymphocytes across endothelial cells in liver and other organs. There is very little information on VAP-1 expression in normal and inflamed lungs. Therefore, we conducted a study to localize VAP-1 in normal mice and human lungs and in two distinct murine models of lung inflammation. Normal mice and human lungs revealed VAP-1 expression in the endothelium of large and mid-sized pulmonary vessels but not in alveolar septae, airway epithelium or blood cells. Mice that lack the lpr(-/-) gene and develop extensive lymphocytic infiltration in their lungs showed VAP-1 expression similar to the normal mice lungs. Mice subjected to cecal ligation and puncture developed acute lung inflammation and showed VAP-1 not only in endothelial cells but also in inflammatory cells in perivascular areas at 72 h after the procedure. We concluded that VAP-1 expression may contribute to the functional heterogeneity of endothelial cells within the lung to create distinct sites for the recruitment of inflammatory cells. Furthermore, since VAP-1 is expressed over a longer period of time in inflamed lungs, it may even be a suitable target for drug delivery and therapeutic manipulations.
Singh
Baljit
B
Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, Canada. baljit.singh@usask.ca
Tschernig
Thomas
T
van Griensven
Martijn
M
Fieguth
Armin
A
Pabst
Reinhard
R
D000368:Aged; D006631:Amine Oxidase (Copper-Containing) / Q000032:analysis*; D000818:Animals; D015815:Cell Adhesion Molecules / Q000032:analysis*; D004730:Endothelium, Vascular / Q000473:pathology; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D007150:Immunohistochemistry; D007223:Infant; D008168:Lung / Q000098:blood supply / Q000737:chemistry*; D008297:Male; D008565:Membrane Proteins / Q000032:analysis; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008875:Middle Aged; D009154:Mutation; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000032:analysis; D011014:Pneumonia / Q000378:metabolism* / Q000473:pathology; D019014:fas Receptor / Q000235:genetics; D062765:Junctional Adhesion Molecule B
D006631:Amine Oxidase (Copper-Containing); D015815:Cell Adhesion Molecules; D007136:Immunoglobulins; D008565:Membrane Proteins; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D019014:fas Receptor; C411887:JAM2 protein, human; D062765:Junctional Adhesion Molecule B; C498781:Jam2 protein, mouse; C076668:AOC3 protein, human