A requirement for anaerobically induced redox functions during aerobic growth of Escherichia coli with serine, glycine and leucine as carbon source.
Escherichia coli strains with mutations in 3 genes coding for redox functions--torA, nuoM and glpC--are able to grow with pyruvate as carbon source, but are not able to use a combination of serine, glycine and leucine as carbon source, unlike the parent strain which uses either. All three mutants are able to produce and activate L-serine deaminase (L-SD) when grown in glucose minimal medium, and thus should be able to convert serine to pyruvate and grow on it. We suggest that activation of L-SD involves specific chemical reactions, perhaps building an Fe-S cluster. Mutant cells can carry out the necessary reaction to activate L-SD when grown in glucose minimal medium but apparently cannot do so when grown in SGL medium.
Lan
Jie
J
Biology Department, Concordia University, 1455 de Maisonneuve Avenue, Montreal, Quebec H3G 1M8, Canada.
Newman
Elaine B
EB
D000693:Anaerobiosis / Q000502:physiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D005816:Genetic Complementation Test; D005998:Glycine / Q000378:metabolism*; D012695:L-Serine Dehydratase; D007930:Leucine / Q000378:metabolism*; D009154:Mutation; D010084:Oxidation-Reduction; D016133:Polymerase Chain Reaction; D012694:Serine / Q000378:metabolism*
D004269:DNA, Bacterial; D012694:Serine; D012695:L-Serine Dehydratase; D007930:Leucine; D005998:Glycine