Conformational behavior of chondroitin and chondroitin sulfate in relation to their physical properties as inferred by molecular modeling.
Chondroitin and chondroitin sulfates belong to the family of glycosaminoglycans. They are most widely distributed in animal tissues, where they are involved in structural functions and in cell-cell communication. Their basic structures consist of a disaccharidic repeating unit of beta-D-glucuronic acid (GlcA) and 2-acetamido-2-deoxy-beta-D-galactose (GalNAc), this latter being sulfated at different positions. Molecular mechanics has been applied to calculate the adiabatic energy maps for each of the constituting disaccharides of chondroitin, chondroitin 4-sulfate, and chondroitin 6-sulfate using the MM3 force field. Based on these maps, higher levels of structural organization have been simulated. On one hand, the disordered state is studied through a Metropolis-based algorithm; the resulting chains present a behavior of semirigid polymers, with an order of stiffness: chondroitin 4-sulfate > chondroitin > chondroitin 6-sulfate. On the other hand, the exploration of the stable ordered forms leads to numerous helical conformations of comparable energies. Several of these conformations correspond to the experimentally observed ones. The ability of coordination with cations has also been explored, resulting in a preferential stereospecificity for calcium ions when compared to sodium ions.
Rodríguez-Carvajal
Miguel A
MA
Centre de Recherches sur les Macromolécules Végétales, CNRS, Grenoble, France.
Imberty
Anne
A
Pérez
Serge
S
D002151:Calorimetry; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002807:Chondroitin / Q000737:chemistry*; D002809:Chondroitin Sulfates / Q000737:chemistry*; D004187:Disaccharides / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry
D004187:Disaccharides; D009844:Oligosaccharides; D002807:Chondroitin; D002809:Chondroitin Sulfates