title: The envelope glycoprotein E2 is a determinant of cell culture tropism in ruminant pestiviruses.
abstract: Bovine viral diarrhoea virus (BVDV) isolates infect cultured Madin-Darby bovine kidney (MDBK) cells as efficiently as sheep kidney cells. In contrast, border disease virus (BDV) propagates poorly in MDBK cells but infects sheep cells very efficiently. The envelope glycoprotein E2 has been shown to be essential for virus infectivity. To explore the potential role of E2 in pestivirus host range in cell cultures, we engineered a chimeric BVDV with the E2 coding region from BDV. As expected, the BVDV-E2(bdv) chimera retained the ability of BDV to multiply in sheep cells but experienced a remarkable reduction in its ability to propagate and form plaques in MDBK, a phenotype that is characteristic of the E2 donor, BDV31 virus. Control chimeric BVDV bearing a type II E2 demonstrated that the heterologous E2 does not impair replication in MDBK or lamb cells. These results establish a role for E2 in determining the tropism of a pestivirus in cell culture.
authors.affiliation: Department of Veterinary & Biomedical Sciences, University of Nebraska-Lincoln, Fair Street & East Campus Loop, Lincoln, NE 68583-0905, USA.
authors.forename: Delin
authors.initials: D
authors.lastname: Liang
authors.affiliation: Department of Veterinary & Biomedical Sciences, University of Nebraska-Lincoln, Fair Street & East Campus Loop, Lincoln, NE 68583-0905, USA.
authors.forename: Ignacio Fernandez
authors.initials: IF
authors.lastname: Sainz
authors.affiliation: Department of Veterinary & Biomedical Sciences, University of Nebraska-Lincoln, Fair Street & East Campus Loop, Lincoln, NE 68583-0905, USA.
authors.forename: Israrul H
authors.initials: IH
authors.lastname: Ansari
authors.affiliation: Department of Veterinary & Biomedical Sciences, University of Nebraska-Lincoln, Fair Street & East Campus Loop, Lincoln, NE 68583-0905, USA.
authors.forename: Laura H V G
authors.initials: LHVG
authors.lastname: Gil
authors.affiliation: Department of Veterinary & Biomedical Sciences, University of Nebraska-Lincoln, Fair Street & East Campus Loop, Lincoln, NE 68583-0905, USA.
authors.forename: Ventzislav
authors.initials: V
authors.lastname: Vassilev
authors.affiliation: Department of Veterinary & Biomedical Sciences, University of Nebraska-Lincoln, Fair Street & East Campus Loop, Lincoln, NE 68583-0905, USA.
authors.forename: Ruben O
authors.initials: RO
authors.lastname: Donis
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018083:Border disease virus / Q000472:pathogenicity*; D002417:Cattle; D002460:Cell Line; D001908:Diarrhea Viruses, Bovine Viral / Q000472:pathogenicity*; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012418:Ruminants / Q000821:virology*; D012756:Sheep; D013045:Species Specificity; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010948:Viral Plaque Assay; D015678:Viral Structural Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014774:Virulence
chemical_list: D006023:Glycoproteins; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins; D015678:Viral Structural Proteins; C085424:glycoprotein E2, border disease virus; C076551:gp53, bovine viral diarrhea virus