title: Improved PCR-based method for the direct screening of Streptomyces transformants.
abstract: Streptomycetes are attractive microorganisms because of their high secretion capacity and for the production of secondary metabolites. We report the improvement of a PCR-based method for screening of solid media-grown Streptomyces transformants. By resuspending mycelium into 2.5-10% dimethyl sulfoxide (DMSO), extensive manipulation prior to PCR could be avoided, thus significantly reducing screening time. Results proved to be both reliable and specific.
authors.affiliation: Laboratory of Bacteriology, Rega Institute, Katholieke Universiteit Leuven, Minderbroedersstraat 10, B-3000 Louvain, Belgium.
authors.forename: W
authors.initials: W
authors.lastname: Van Dessel
authors.forename: L
authors.initials: L
authors.lastname: Van Mellaert
authors.forename: N
authors.initials: N
authors.lastname: Geukens
authors.forename: J
authors.initials: J
authors.lastname: Anné
mesh_terms: D004121:Dimethyl Sulfoxide / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013302:Streptomyces / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D014169:Transformation, Bacterial
chemical_list: D004121:Dimethyl Sulfoxide