title: Exercise-induced HSP27, HSP70 and MAPK responses in human skeletal muscle.
abstract: OBJECTIVE
  The present work examined protein and messenger RNA (mRNA) expression of intramuscular heat shock protein 27 (HSP27), heat shock cognate (HSC70) and HSP70 in human biceps brachii (BB) and vastus lateralis (VL) subsequent to two different exercises.
  
  
  METHODS
  Untrained subjects performed 50 high-force eccentric contractions with their non-dominant BB and ran downhill (-10 degrees) for 30 min. The 48-h PX stress response was evaluated with immunoblotting and reverse transcriptase-polymerase chain reaction (RT-PCR). Muscle damage was indicated indirectly at 48 h post-exercise (PX) [loss of mobility, muscle soreness and serum creatine kinase (CK) activity].
  
  
  RESULTS
  On the protein level, HSP27 and HSP70 increased significantly PX in the BB (384 and 227%, respectively; P < 0.01), but there were no significant HSP changes in the VL or in HSC70 in either muscle. The RT-PCR data complemented these findings: BB HSP27 and HSP70C mRNA levels increased (135 and 128%, respectively; P < 0.05); in the VL only HSP70B increased (206%; P < 0.05). Phosphorylation of e-jun NH2-terminal kinase (JNK) and extracellular regulated kinase (ERK) increased significantly in the BB (226 and 200%, respectively; P < 0.05) but not in the VL, indicating activation of these pathways only after the resistance exercise.
  
  
  CONCLUSIONS
  These data indicate that the PX HSP and mitogen-activated protein kinase responses are exercise-specific and local, not systemic. Further, only the resistance exercise induced HSP expression (protein and mRNA) and JNK/ERK activation at 48 h PX, suggesting that these molecules may be important to long-term skeletal muscle adaptations such as hypertrophy.
authors.affiliation: Molecular and Cellular Biology Program, University of Massachusetts, Amherst, MA, USA.
authors.forename: H S
authors.initials: HS
authors.lastname: Thompson
authors.forename: E B
authors.initials: EB
authors.lastname: Maynard
authors.forename: E R
authors.initials: ER
authors.lastname: Morales
authors.forename: S P
authors.initials: SP
authors.lastname: Scordilis
mesh_terms: D000199:Actins / Q000032:analysis; D001132:Arm; D015444:Exercise / Q000502:physiology*; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting; D007866:Leg; D008297:Male; D020928:Mitogen-Activated Protein Kinases / Q000097:blood*; D018482:Muscle, Skeletal / Q000201:enzymology / Q000293:injuries / Q000378:metabolism*; D010766:Phosphorylation; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
chemical_list: D000199:Actins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D020928:Mitogen-Activated Protein Kinases