The role of p55CDC in cell cycle control and mammalian cell proliferation, differentiation, and apoptosis.
The p55CDC (cell division cycle) protein is a key regulator of the cell cycle. p55CDC is related to both the CDC20 and the CDH1 proteins in yeast. p55CDC has been shown to activate the ubiquitin ligase anaphase promoting complex (APC), which is involved in degradation of proteins that control mitosis. To define the role of p55CDC during the mammalian cell cycle, we overexpressed this protein in the murine myeloid cell line 32Dcl3. 32Dcl3 cells are an ideal model system because these cells can be induced to proliferate, differentiate, or activate cellular programs leading to apoptosis. Our work suggests that p55CDC participates in cell growth, maturation, and death. Thus, p55CDC may play a more diverse role in modulating cellular functions in addition to controlling the cell cycle.
Lin
Michael
M
Department of Pediatrics, Mattel Children's Hospital at University of California at Los Angeles, Gwynne Hazen Cherry Memorial Laboratories, Jonsson Comprehensive Cancer Center, David Geffen School of Medicine, Los Angeles, CA 90095-1752, USA.
Chang
Johnny K
JK
Shankar
Deepa
D
Sakamoto
Kathleen M
KM
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D064199:Cdc20 Proteins; D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology*; D018797:Cell Cycle Proteins / Q000502:physiology*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002455:Cell Division / Q000187:drug effects / Q000502:physiology*; D002460:Cell Line; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006098:Granulocytes / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008938:Mitosis; D022423:Myeloid Cells / Q000378:metabolism; D016196:S Phase; D008941:Spindle Apparatus / Q000378:metabolism
D064199:Cdc20 Proteins; C577491:Cdc20 protein, mouse; D018797:Cell Cycle Proteins; D016179:Granulocyte Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor