title: Analysis of a shared pancreatic cancer antigen recognized by an HLA-A*2601-restricted cytotoxic T-lymphocyte clone.
abstract: BACKGROUND
  We have generated HLA-A*2601-restricted CD8+ CTL clones against an autologous pancreatic cancer cell line.
  
  
  OBJECTIVE
  To characterize the antigen expressed on the cancer cells.
  
  
  METHODS
  We assessed cytotoxic activities and cytokine production of these CTL clones reacting against cancer cell lines that stably or transiently expressed the HLA-A*2601 gene.
  
  
  RESULTS
  These CTL clones recognized 4 of 10 allogeneic pancreatic cancer cell lines and a gallbladder cancer cell line in the context of HLA-A*2601. However, the CTL clones did not recognize three hepatocellular carcinoma cell lines, two esophageal squamous cell carcinoma cell lines, or a lung adenocarcinoma cell line.
  
  
  CONCLUSIONS
  Thus, the CTL clones may recognize a shared, but not ubiquitously expressed, tumor antigen on pancreatic and gallbladder cancer cells.
authors.affiliation: Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, Japan.
authors.forename: Kiyoteru
authors.initials: K
authors.lastname: Kashiwagi
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Ikeda
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Hirohashi
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Satomi
authors.initials: S
authors.lastname: Idenoue
authors.forename: Itaru
authors.initials: I
authors.lastname: Hirai
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Kamiguchi
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Tamura
authors.forename: Toshiko
authors.initials: T
authors.lastname: Torigoe
authors.forename: Yshimasa
authors.initials: Y
authors.lastname: Wada
authors.forename: Koichi
authors.initials: K
authors.lastname: Hirata
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Sato
mesh_terms: D000951:Antigens, Neoplasm / Q000032:analysis / Q000276:immunology*; D045744:Cell Line, Tumor; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D005838:Genotype; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D015234:HLA-A Antigens / Q000235:genetics / Q000276:immunology*; D006801:Humans; D020014:K562 Cells; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology*
chemical_list: D000951:Antigens, Neoplasm; D015234:HLA-A Antigens; C039683:HLA-A*26 antigen; D016178:Granulocyte-Macrophage Colony-Stimulating Factor