Isolation and biological characterization of a 6-kDa protein from hepatopancreas of lobster Panulirus argus with insulin-like effects.
A protein with insulin-like effects was isolated from the hepatopancreas of the lobster Panulirus argus following a classic method for mammalian insulin purification from the pancreas. After acid-alcoholic extraction and ethanol-ether precipitation followed by molecular filtration chromatography, a protein with an apparent molecular weight of 6 kDa was isolated. This protein is characterized by its ability to interact with anti-insulin antibodies and by mimicking insulin actions as the stimulation of glucose oxidation to CO(2) and lipogenesis in isolated rat adipocytes. In addition, this insulin immunoreactive protein (IIP) was able to stimulate the autophosphorylation of the insulin receptor present in rat adipocyte plasma membranes, in a dose-dependent manner. The immunological and biochemical results obtained are consistent with the hypothesis that protein(s) with insulin-like effects occur in the digestive gland of the lobster P. argus and may be of significance to control metabolic and growth related processes in crustaceans.
Gallardo
Nilda
N
Area de Bioquímica, Facultad de Químicas, Centro Regional de Investigaciones Biomédicas, Universidad de Castilla-La Mancha, Campus Universitario s/n, 13071 Ciudad Real, Spain.
Carrillo
Olimpia
O
Moltó
Eduardo
E
Deás
Marta
M
González-Suárez
Roberto
R
Carrascosa
José M
JM
Ros
Manuel
M
Andrés
Antonio
A
D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D001667:Binding, Competitive / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002850:Chromatography, Gel; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D005947:Glucose / Q000378:metabolism; D043143:Hepatopancreas / Q000737:chemistry*; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D015151:Immunoblotting; D066298:In Vitro Techniques; D007328:Insulin / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D033521:Palinuridae / Q000378:metabolism*; D010766:Phosphorylation; D011863:Radioimmunoassay; D051381:Rats; D011972:Receptor, Insulin / Q000187:drug effects; D014443:Tyrosine / Q000378:metabolism
D007004:Hypoglycemic Agents; D007328:Insulin; C480586:insulin-like protein 6-kDa, Panulirus argus; D014443:Tyrosine; D011972:Receptor, Insulin; D005947:Glucose