Association between the Pl(A) platelet glycoprotein GPIIIa polymorphism and extent of coronary artery disease.
BACKGROUND
The Pl(A2) allele of the gene encoding for GPIIIa subunit of the platelet membrane receptor glycoprotein (GP) IIb/IIIa has been suggested as a significant risk factor for thrombotic complications of coronary artery disease (CAD). The aim of the current investigation was to investigate the association between Pl(A) GPIIIa polymorphism and the extent of angiographically confirmed CAD in patients from the north region of Poland.


METHODS
The study was performed in 397 male Caucasian patients. All subjects had significant coronary artery stenosis confirmed by elective coronary angiography. Screening for the Pl(A) GPIIIa genotypes was performed by polymerase chain reaction of genomic DNA, followed by NciI digestion and agarose gel electrophoresis.


RESULTS
The genotype distribution of the Pl(A) GPIIIa polymorphism in our study group was Pl(A1/A1)-75%, Pl(A1/A2)-24% and Pl(A2/A2)-1% with Pl(A1) and Pl(A2) allele frequencies of 0.87 and 0.13, respectively. The prevalence of the homozygous Pl(A1/A1) genotype among subjects with multiple-vessel CAD (two or three vessels with at least 50% stenosis) was significantly higher than in patients with single-vessel disease; the odds ratio of Pl(A2/A2) or Pl(A1/A2) patients for having multiple-vessel CAD was 0.46 (95% CI 0.27-0.77, P<0.01). The mean CAD score for Pl(A1/A1) patients was significantly higher in comparison to Pl(A2/A2) and Pl(A1/A2) patients (7.58+/-2.20 and 6.98+/-2.37, respectively, P<0.05).


CONCLUSIONS
Our results suggest, that the Pl(A1/A1) genotype of Pl(A) GPIIIa polymorphism is associated with more severe CAD in male Caucasian patients from the north region of Poland.
Gruchała
Marcin
M
First Department of Cardiology, Medical University of Gdansk, Poland.
Ciećwierz
Dariusz
D
Ochman
Karolina
K
Targoński
Radosław
R
Dubaniewicz
Witold
W
Sobiczewski
Wojciech
W
Wasag
Bartosz
B
Drewla
Piotr
P
Skarzynski
Pawel
P
Romanowski
Piotr
P
Limon
Janusz
J
Rynkiewicz
Andrzej
A
D000328:Adult; D000368:Aged; D017023:Coronary Angiography; D003324:Coronary Artery Disease / Q000000981:diagnostic imaging / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics*; D008875:Middle Aged; D010960:Plasminogen Activators / Q000235:genetics*; D011044:Poland; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D012720:Severity of Illness Index; D044465:White People / Q000235:genetics*
D008562:Membrane Glycoproteins; C040580:glycoprotein GP III; D010960:Plasminogen Activators