Role of biomodulators and involvement of protein tyrosine kinase on stem cell migration in normal and leukaemic mice.
Tyrosine kinase has an important role with regard to self-renewal and as a comitogen in the movement of stem cells out of the haemopoietic stem cell pool into the progeny pool. The present investigation has an objective to evaluate the protein tyrosine kinase (PTK) activity of bone marrow derived pluripotent cells before and after application of biological response modifiers (BRMs) in normal and leukaemic mice. The PTK activity of the cytosolic fraction of bone marrow cells has been determined by the assay kit based on per-oxidase labeled substrate analog and biotin-streptavidin expression. A consequent cell population kinetic study has also been conducted. Results showed a higher activity in the cells of leukaemic mice, which under the influence of interleukin-2 (IL-2) and the non-specific BRM sheep erythrocytes (SRBC) undergo further activation. Interferon-gamma (IFN-gamma) when administered alone showed a suppressive effect and the combination of the three manifested a resultant suppression. Corresponding migration (cell population kinetics) of the bone marrow cells (BMC) also correlated well with the PTK activity of the cells concerned. The observations indicated that the pluripotent BMCs are under regulated control of the PTK activity, which can be manipulated by selective BRMs. The data also suggested the therapeutic benefit of IFN-gamma along with chemotherapeutics against leukaemia and that of IL-2 and SRBC during bone marrow failure.
Law
Sujata
S
Department of Haematology, Immunology and Immuno-Haematology Laboratory, School of Tropical Medicine, CR Avenue, Kolkata 700 073, India.
Maiti
D
D
Palit
A
A
Chaudhuri
S
S
D000477:Alkylating Agents / Q000633:toxicity; D000818:Animals; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D005038:Ethylnitrosourea / Q000633:toxicity; D005260:Female; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000201:enzymology*; D007155:Immunologic Factors / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology; D007938:Leukemia / Q000139:chemically induced / Q000201:enzymology*; D008297:Male; D051379:Mice; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology
D000477:Alkylating Agents; D007155:Immunologic Factors; D007376:Interleukin-2; D007371:Interferon-gamma; D011505:Protein-Tyrosine Kinases; D005038:Ethylnitrosourea