Differential regulation of metallothionein-I, II, and III mRNA expression in the rat brain following kainic acid treatment.
Although metallothioneins (MTs) are believed to be involved in the protection against neural stresses, spatio-temporal regulation of MT isoforms following neural insults has not been thoroughly examined. In this study, we found that systemic application of kainic acid (KA) rapidly induced MT-I and II expression in neurons localized in hippocampal formation, piriform cortex, and amygdala of the adult rat, whereas the level of MT-III mRNA was decreased in KA-vulnerable areas. At 96 h after KA treatment, while the neuronal expression of MT-I and II returned to basal level, the glial expression of MT-I, II and III was increased in the reactive astrocytes. Differential regulation of MT isoforms in neuron and gila suggests that each isoform might have distinct role in the cell-type dependent cellular responses against KA-evoked neural injuries.
Kim
Donghou
D
1Department of Anatomy and Cell Biology, College of Medicine, University of Ulsan, 388-1 Poongnap-Dong, Songpa-Gu, Seoul, Korea 138-736.
Kim
Eun Hae
EH
Kim
Changmee
C
Sun
Woong
W
Kim
Hyun Jung
HJ
Uhm
Chang-Sub
CS
Park
Sun-Hwa
SH
Kim
Hyun
H
D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism; D001921:Brain / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D017403:In Situ Hybridization; D007608:Kainic Acid / Q000494:pharmacology*; D008297:Male; D008668:Metallothionein / Q000235:genetics* / Q000378:metabolism; D000090023:Metallothionein 3; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000090023:Metallothionein 3; D009419:Nerve Tissue Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; D008668:Metallothionein; D007608:Kainic Acid