Same serial section correlative light and energy-filtered transmission electron microscopy.
Correlative imaging of a specific cell with both the light microscope and the electron microscope has proved to be a difficult task, requiring enormous amounts of patience and technical skill. We describe a technique with a high rate of success, which can be used to identify a particular cell in the light microscope and then to embed and thin-section it for electron microscopy. The technique also includes a method to obtain many uninterrupted, thin serial sections for imaging by conventional or energy-filtered transmission electron microscopy, to obtain images for 3D analysis of detail at the suborganelle level.
Ren
Ying
Y
Programme in Cell Biology, The Hospital for Sick Children, Toronto, Ontario, Canada.
Kruhlak
Michael J
MJ
Bazett-Jones
David P
DP
D005455:Fluorescent Antibody Technique; D005629:Frozen Sections; D006801:Humans; D008854:Microscopy, Electron / Q000379:methods*; D013057:Spectrum Analysis / Q000379:methods*; D016610:Tissue Embedding; D014407:Tumor Cells, Cultured / Q000648:ultrastructure*