[Antidiuretic reaction of the human and rat kidney to peroral administration of arginine-vasopressin and desmopressin].
Water in amount of 5 ml/100 g body weight was administered through a gastric probe into the stomach in alert rats; subjects-volunteers drank 20 ml of water per 1 kg of body weight. This resulted in diuresis at the peak of which the excreted water fraction reached 23% in rats and 12.4% in human subjects, whereas excretion of the osmotically free water amounted to 0.103 +/- 0.018 ml/min/100 g body weight and 10.0 +/- 1.8 ml/min/1.73 m2 of the body surface, respectively. These data indicate a practically complete inhibition of the arginine vasopressin secretion. On intragastric administration of 10 micrograms of arginine vasopressin or 0.2 microgram of desmopressin, with water in rats, a prolonged and quite obvious antidiuretic response occurred, with a marked increase of reabsorption of the osmotically free water in kidneys. A direct correlation has been found between the dose of the intragastrically administered vasopressin in the dose range from 0.1 to 10 micrograms/100 g body weight and a decrease of clearance of the osmotically free water. In subjects volunteers, an antidiuretic reaction to administration of 0.2 mg of desmopressin with water, was found. The data obtained provide a direct proof of intestinal absorption of nanopeptides without loss of their physiological activity. Significance of the data obtained for physiology of digestion and for clinical medicine, is discussed.
Natochin
Iu V
IuV
I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Acad. Sci., 194223, St. Petersburg, pr. M. Toreza, 44, Russia.
Grigor'ev
A I
AI
Buravkova
L B
LB
Larina
I M
IM
Prutskova
N P
NP
Shakhmatova
E I
EI
D000284:Administration, Oral; D000293:Adolescent; D000328:Adult; D000818:Animals; D001127:Arginine Vasopressin / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000494:pharmacology; D003894:Deamino Arginine Vasopressin / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000494:pharmacology; D004231:Diuresis / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007273:Injections, Intramuscular; D007408:Intestinal Absorption; D007668:Kidney / Q000187:drug effects*; D008297:Male; D008875:Middle Aged; D051381:Rats; D017208:Rats, Wistar; D012076:Renal Agents / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000494:pharmacology; D014867:Water / Q000008:administration & dosage
D012076:Renal Agents; D014867:Water; D001127:Arginine Vasopressin; D003894:Deamino Arginine Vasopressin
Antidiureticheskaia reaktsiia pochek cheloveka i krysy pri peroral'nom vvedenii arginin-vazopressina i desmopressina.