title: Immunochemical studies on the O-antigens of Proteus mirabilis O23 and Proteus vulgaris O23.
abstract: Analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy demonstrated that the O-specific polysaccharides of Proteus mirabilis PrK 42/57 and P. vulgaris PrK 43/57 are structurally similar to that of P. vulgaris PrK 44/57 and different from the polysaccharide of P. mirabilis PrK 41/57 studied earlier. The lipopolysaccharides of these strains were tested using enzyme immunosorbent assay, passive hemolysis and Western blot with O-antisera against P. mirabilis 42/57 and P. vulgaris 43/57 and 44/57, as well as with cross-absorbed O-antisera. The chemical and serological data revealed the basis for combining the four strains into Proteus serogroup O23 and division of this serogroup to three subgroups, one for P. vulgaris 43/57 and 44/57 and two others for P. mirabilis 41/57 and 42/57.
authors.affiliation: Department of Immunobiology of Bacteria, Institute of Microbiology and Immunology, University of Lódź, Banacha 12/16, 90-237 Lódź, Poland. rozala@biol.uni.lodz.pl
authors.forename: Antoni
authors.initials: A
authors.lastname: Rózalski
authors.forename: Andrei V
authors.initials: AV
authors.lastname: Perepelov
authors.forename: Beata
authors.initials: B
authors.lastname: Bartodziejska
authors.forename: Sof'ya N
authors.initials: SN
authors.lastname: Senchenkova
authors.forename: Dorota
authors.initials: D
authors.lastname: Babicka
authors.forename: Wiesław
authors.initials: W
authors.lastname: Kaca
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
mesh_terms: D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D019906:Nuclear Magnetic Resonance, Biomolecular; D019081:O Antigens / Q000737:chemistry* / Q000276:immunology*; D011513:Proteus mirabilis / Q000737:chemistry* / Q000276:immunology; D011514:Proteus vulgaris / Q000737:chemistry* / Q000276:immunology
chemical_list: D019081:O Antigens