[IgM-ELISA for diagnosis of schistosomiasis mansoni in low endemic areas].
An immunoenzymatic method for the detection of IgM antibodies (IgM-ELISA) against a fraction of Schistosoma mansoni adult worm antigen, soluble in trichloroacetic acid (TCA-soluble fraction), was evaluated for epidemiological purposes in low endemic areas for schistosomiasis. Blood samples on filter paper were collected from a population living in the municipality of Pedro de Toledo, São Paulo State, and submitted to IgM-ELISA. The results were compared to those obtained by the IgM-immunofluorescence test (IgM-IFT) and the Kato-Katz parasitological method. Positive rates of 36.8%, 33.5%, and 1.6% were obtained respectively by the IgM-ELISA, IgM-IFT, and Kato-Katz methods. The geometric mean obtained by the parasitological method was 40.9 eggs per gram of feces (epg). The nearly perfect agreement observed between the two serological tests (Kappa index of 0,89) indicates good diagnostic performance by the evaluated test. IgM-ELISA is a potentially useful method for diagnosis of schistosomiasis in individuals with low worm burden.
de Oliveira
Edward José
EJ
Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, SP, 05508-900, Brasil. edwardoliveira@bol.com.br
Kanamura
Hermínia Yoshio
HY
Dias
Luiz Cândido de Souza
LC
Soares
Lanny Cristina Burlandy
LC
Lima
Dirce Mary Correia
DM
Ciaravolho
Ricardo Mário de Carvalho
RM
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood* / Q000302:isolation & purification; D019353:Endemic Diseases; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005243:Feces / Q000469:parasitology; D005260:Female; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood* / Q000276:immunology; D008297:Male; D010270:Parasite Egg Count; D012550:Schistosoma mansoni / Q000276:immunology; D012555:Schistosomiasis mansoni / Q000097:blood / Q000175:diagnosis* / Q000276:immunology
D000909:Antibodies, Helminth; D007075:Immunoglobulin M
ELISA-IgM para diagnóstico da esquistossomose mansoni em área de baixa endemicidade.