Mycobacterium shottsii sp. nov., a slowly growing species isolated from Chesapeake Bay striped bass (Morone saxatilis).
Slowly growing, non-pigmented mycobacteria were isolated from striped bass (Morone saxatilis) during an epizootic of mycobacteriosis in the Chesapeake Bay. Growth characteristics, acid-fastness and results of 16S rRNA gene sequencing were consistent with those of the genus Mycobacterium. A unique profile of biochemical reactions was observed among the 21 isolates. A single cluster of eight peaks identified by analysis of mycolic acids (HPLC) resembled those of reference patterns but differed in peak elution times from profiles of reference species of the Mycobacterium tuberculosis complex. One isolate (M1 75T) was placed within the slowly growing mycobacteria by analysis of aligned 168S rRNA gene sequences and was proximate in phylogeny to Mycobacterium ulcerans and Mycobacterium marinum. However, distinct nucleotide differences were detected in the 16S rRNA gene sequence among M175T, M. ulcerans and M. marinum (99.2% similarity). Isolate M175T could be differentiated from other slowly growing, non-pigmented mycobacteria by its inability to grow at 37 degrees C, production of niacin and urease, absence of nitrate reductase and resistance to isoniazid (1 microg ml(-1)), thiacetazone and thiophene-2-carboxylic hydrazide. Based upon these genetic and phenotypic differences, isolate M175T (=ATCC 700981T =NCTC 13215T) is proposed as the type strain of a novel species, Mycobacterium shottsii sp. nov.
Rhodes
Martha W
MW
Virginia Institute of Marine Science, College of William and Mary, PO Box 1346, Gloucester Point, VA 23062, USA.
Kator
Howard
H
Virginia Institute of Marine Science, College of William and Mary, PO Box 1346, Gloucester Point, VA 23062, USA.
Kotob
Shaban
S
Virginia Institute of Marine Science, College of William and Mary, PO Box 1346, Gloucester Point, VA 23062, USA.
van Berkum
Peter
P
United States Department of Agriculture, Beltsville, MD, USA.
Kaattari
Ilsa
I
Virginia Institute of Marine Science, College of William and Mary, PO Box 1346, Gloucester Point, VA 23062, USA.
Vogelbein
Wolfgang
W
Virginia Institute of Marine Science, College of William and Mary, PO Box 1346, Gloucester Point, VA 23062, USA.
Quinn
Frederick
F
Tuberculosis/Mycobacteriology Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Floyd
Margaret M
MM
Tuberculosis/Mycobacteriology Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Butler
W Ray
WR
Tuberculosis/Mycobacteriology Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Ottinger
Christopher A
CA
US Geological Survey, Leetown Science Center, National Fish Health Research Laboratory, Kearneysville, WV, USA.
D000818:Animals; D001492:Bass / Q000382:microbiology*; D005393:Fish Diseases / Q000453:epidemiology / Q000382:microbiology*; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D009164:Mycobacterium Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D010641:Phenotype; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000032:analysis* / Q000235:genetics; D017422:Sequence Analysis, DNA
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S