Pharmacologically regulated induction of silent mutations (PRISM): combined pharmacological and genetic approaches for learning and memory.
Mouse transgenic and knock-out approaches have made fundamental contributions to our understanding of the molecular and cellular bases of learning and memory. These approaches have successfully identified a large number of molecules with either a central or modulatory role in learning and memory. However, there are limitations associated with first-generation mutant mice, which include, for example, the lack of temporal control over the mutation. Recent technical developments have started to address some of these shortcomings. Here, the authors review a newly developed inducible approach that takes advantage of synergistic interactions between subthreshold genetic and pharmacological manipulations. This approach is easily set up and can be used to study the functional interactions between molecules in signaling pathways.
Frankland
Paul W
PW
Department of Neurobiology, University of California, Los Angeles, USA.
Ohno
Masuo
M
Takahashi
Eiki
E
Chen
Adele R
AR
Costa
Rui M
RM
Kushner
Steven A
SA
Silva
Alcino J
AJ
D000818:Animals; D011905:Genes, ras / Q000235:genetics; D007858:Learning / Q000502:physiology*; D008568:Memory / Q000502:physiology*; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics; D008967:Molecular Biology / Q000379:methods*; D009154:Mutation; D009473:Neuronal Plasticity / Q000502:physiology; D010600:Pharmacology
D020928:Mitogen-Activated Protein Kinases