Automated ribotyping and pulsed-field gel electrophoresis for rapid identification of multidrug-resistant Salmonella serotype newport.
In a series of 116 Salmonella enterica Newport isolates that included 64 multidrug-resistant (MDR) isolates, automated ribotyping and pulsed-field gel electrophoresis (PFGE) discriminated MDR S. Newport with a sensitivity of 100% and 98% and specificity of 76% and 89%, respectively. Clustering of PFGE patterns (but not ribotyping) linked human and bovine cases. Automated ribotyping rapidly identified the MDR strain, and PFGE detected associations that aided epidemiologic investigations.
Fontana
John
J
Massachusetts Department of Public Health, Jamaica Plain, Massachusetts, USA. john.fontana@state.ma.us
Stout
Alison
A
Bolstorff
Barbara
B
Timperi
Ralph
R
D000818:Animals; D002417:Cattle; D024901:Drug Resistance, Multiple, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D021521:Ribotyping / Q000379:methods*; D019779:Salmonella enterica / Q000145:classification* / Q000187:drug effects; D012680:Sensitivity and Specificity