[Peptide hydrolases with catalytic dyad Ser-Lys. Similarity and distinctions of the active centers of ATP-dependent Lon proteases, LexA repressors, signal peptidases and C-terminal processing proteases].
It is established that ATP-dependent protease Lon family belongs to the serine-lysine peptide hydrolases clan. Significant similarity of amino acid sequences of proteases Lon and repressors LexA in the regions including the catalytic serine and lysine residues is revealed by comparing primary structures of different families of the enzymes with Ser-Lys catalytic dyad. The both Lon and LexA families are shown to be divided into two subfamilies in accordance with the nature of amino acids in the catalytically active serine environment. Putative DNA binding sites are revealed in proteolytic domains of Lon A subfamily. Similarities and distinctions of the all families peptide hydrolases of the clan in the regions of their active centers are discussed.
Rotanova
T V
TV
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Siences, Ul. Miklukho-Maklaya 16/10, GSP Moscow, 117997 Russia. rotanova@enzyme.siobc.ras.ru
D049069:ATP-Dependent Proteases; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D020134:Catalytic Domain; D010450:Endopeptidases / Q000737:chemistry* / Q000235:genetics; D006360:Heat-Shock Proteins / Q000737:chemistry* / Q000235:genetics; D008239:Lysine / Q000737:chemistry*; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D012694:Serine / Q000737:chemistry*; D012697:Serine Endopeptidases / Q000737:chemistry* / Q000235:genetics; D000099160:LexA Repressor Protein
D049069:ATP-Dependent Proteases; D001426:Bacterial Proteins; D010450:Endopeptidases; D006360:Heat-Shock Proteins; D008239:Lysine; D008565:Membrane Proteins; D012694:Serine; D012697:Serine Endopeptidases; D000099160:LexA Repressor Protein; C072135:C-terminal processing peptidase; C026515:type I signal peptidase
Peptidgidrolazy s kataliticheskoĭ diadoĭ Ser-Lys. Skhodstvo i razlichiia aktivnykh tsentrov ATP-zavisimykh Lon-proteinaz, repressorov LexA, signal'nykh peptidaz i proteinaz C-kontsevogo protsessinga.