title: Collagen-PVP, a collagen synthesis modulator, decreases intraperitoneal adhesions.
abstract: BACKGROUND
  Adhesion formation in the peritoneal cavity is the most common cause of intestinal obstruction and secondary female infertility. A great effort has been dedicated to reduce adhesion formation because of the associated morbidity and its complications.
  
  
  METHODS
  This study was designed as a before-after comparative trial and included 14 rabbits, with a weight between 300 and 500 g. All rabbits were appendectomized and 1 month later laparotomized to assess adhesion formation. Rabbits were randomized into two groups, Group I (control group), with no intervention, and Group II (experimental group), treated with an intraperitoneal sponge of collagen-polyvinylpyrrolidone (Clg-PVP). The laparotomy procedure was repeated 1 month later for a new assessment of adhesion formation and histological evaluation by H-E and Masson staining.
  
  
  RESULTS
  Histological findings showed abundant infiltrate in the control group, which was mild in the experimental group. With the Masson stain the control group showed a significantly higher amount of collagen than the experimental group and the fibrous tissue was more compact. We found a mean number of adhesions of 3.29 +/- 1.98 for the control group, which decreased to 2.57 +/- 0.79 after the second laparotomy. For the experimental group the mean number of adhesions decreased from 1.86 +/- 0.90 to 0.71 +/- 0.49 after the second laparotomy, with no statistical difference between both groups before Clg-PVP application, but a significant statistical difference after the implantation of Clg-PVP (Student's t test; P < 0.001, two-tailed).
  
  
  CONCLUSIONS
  Collagen-polyvinylpyrrolidone decreases the incidence and size of intraabdominal adhesions after secondary adhesion formation after appendectomy.
authors.affiliation: Department of Surgery, School of Medicine, Universidad Panamericana, A.C. Donatello 75, Insurgentes-Mixcoac, 03920, Mexico City, Mexico. ccervans@mx.up.mx
authors.forename: Carlos R
authors.initials: CR
authors.lastname: Cervantes-Sánchez
authors.forename: Enrique
authors.initials: E
authors.lastname: Olaya
authors.forename: Manuel
authors.initials: M
authors.lastname: Testas
authors.forename: Norberto
authors.initials: N
authors.lastname: Garcia-López
authors.forename: Gabriel
authors.initials: G
authors.lastname: Coste
authors.forename: Gerardo
authors.initials: G
authors.lastname: Arrellin
authors.forename: Adrián
authors.initials: A
authors.lastname: Luna
authors.forename: Fernando Edgar
authors.initials: FE
authors.lastname: Krötzsch
mesh_terms: D000818:Animals; D001062:Appendectomy / Q000009:adverse effects; D003094:Collagen / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000627:therapeutic use*; D005260:Female; D008297:Male; D010532:Peritoneal Diseases / Q000209:etiology / Q000473:pathology / Q000517:prevention & control* / Q000601:surgery; D011205:Povidone / Q000627:therapeutic use*; D011817:Rabbits; D055502:Secondary Prevention; D000267:Tissue Adhesions / Q000209:etiology / Q000473:pathology / Q000517:prevention & control / Q000601:surgery
chemical_list: C120488:collagen-PVP; D003094:Collagen; D011205:Povidone