Identification of a new genotype H wild-type mumps virus strain and its molecular relatedness to other virulent and attenuated strains.
A single clinical isolate of mumps virus designated 88-1961 was obtained from a patient hospitalized with a clinical history of upper respiratory tract infection, parotitis, severe headache, fever and lymphadenopathy. We have sequenced the full-length genome of 88-1961 and compared it against all available full-length sequences of mumps virus. Based upon its nucleotide sequence of the SH gene 88-1961 was identified as a genotype H mumps strain. The overall extent of nucleotide and amino acid differences between each individual gene and protein of 88-1961 and the full-length mumps samples showed that the missense to silent ratios were unevenly distributed. Upon evaluation of the consensus sequence of 88-1961, four positions were found to be clearly heterogeneous at the nucleotide level (NP 315C/T, NP 318C/T, F 271A/C, and HN 855C/T). Sequence analysis revealed that the amino acid sequences for the NP, M, and the L protein were the most conserved, whereas the SH protein exhibited the highest variability among the compared mumps genotypes A, B, and G. No identifying molecular patterns in the non-coding (intergenic) or coding regions of 88-1961 were found when we compared it against relatively virulent (Urabe AM9 B, Glouc1/UK96, 87-1004 and 87-1005) and non-virulent mumps strains (Jeryl Lynn and all Urabe Am9 A substrains).
Amexis
Georgios
G
Center for Biologics Evaluation and Research, FDA, Rockville, Maryland, USA. amexis@nih.gov
Rubin
Steven
S
Chatterjee
Nando
N
Carbone
Kathryn
K
Chumakov
Kostantin
K
D000818:Animals; D002460:Cell Line; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D009107:Mumps / Q000821:virology*; D009109:Mumps virus / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity*; D014764:Viral Proteins / Q000235:genetics; D014774:Virulence
D014764:Viral Proteins; C067386:small hydrophobic protein, Mumps virus