title: Mesenchymal stem cells from rat visceral fat exhibit multipotential differentiation in vitro.
abstract: Human subcutaneous fat-derived stem cells were recently shown to have the potential to differentiate in vitro into a variety of cell types, including adipocytes, osteoblasts, chondrocytes, and myoblasts (Zuk et al., Tissue Eng. 2001;7:211-228). Subcutaneous adipose tissue may therefore prove to be an easily acquired and abundant source of stem cells. Presently it is unclear whether mammals such as rats (which possess small or nonexistent subcutaneous fat pads) contain mesenchymal stem cells within the visceral fat of the abdominal cavity, or whether the visceral fat of any species contains stem cells. In this study we isolated and expanded a pool of mesenchymal cells from visceral fat of adult Sprague-Dawley rats and induced their differentiation in vitro into adipocytes, osteoblasts, neural cells, and chondrocytes. The differentiated phenotypes were verified by morphology as well as detection and expression of tissue-specific protein and mRNA. We conclude that despite well-documented differences in the metabolic and biochemical properties among anatomically distinct depots of fat, the visceral fat of rats contains adult mesenchymal stem cells with developmental potential similar to those isolated from subcutaneous fat in humans. Therefore, animals such as rats provide both a source of fat-derived stem cells and an immunocompetent, autologous host animal in which to investigate the capacity of the fat-derived cells to differentiate and form tissues in vivo.
authors.affiliation: Department of Plastic and Reconstructive Surgery, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908, USA.
authors.forename: S S
authors.initials: SS
authors.lastname: Tholpady
authors.forename: A J
authors.initials: AJ
authors.lastname: Katz
authors.forename: R C
authors.initials: RC
authors.lastname: Ogle
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000502:physiology; D000273:Adipose Tissue / Q000166:cytology* / Q000254:growth & development*; D000818:Animals; D015415:Biomarkers; D002454:Cell Differentiation / Q000502:physiology*; D019070:Cell Lineage / Q000502:physiology; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000502:physiology; D024043:Collagen Type II / Q000235:genetics; D008297:Male; D008648:Mesoderm / Q000166:cytology* / Q000502:physiology; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000502:physiology; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology; D015676:Osteonectin / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D014781:Viscera / Q000166:cytology / Q000254:growth & development; D016875:tau Proteins / Q000235:genetics
chemical_list: D015415:Biomarkers; D024043:Collagen Type II; D015676:Osteonectin; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors; D016875:tau Proteins