title: Zygosaccharomyces kombuchaensis, a new ascosporogenous yeast from 'Kombucha tea'.
abstract: A new ascosporogenous yeast, Zygosaccharomyces kombuchaensis sp. n. (type strain NRRL YB-4811, CBS 8849), is described; it was isolated from Kombucha tea, a popular fermented tea-based beverage. The four known strains of the new species have identical nucleotide sequences in domain D1/D2 of 26S rDNA. Phylogenetic analysis of D1/D2 and 18S rDNA sequences places Z. kombuchaensis near Zygosaccharomyces lentus. The two species are indistinguishable on standard physiological tests used for yeast identification, but can be recognized from differences in restriction fragment length polymorphism patterns obtained by digestion of 18S-ITS1 amplicons with the restriction enzymes DdeI and MboI.
authors.affiliation: Microbial Properties Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 N. University Street, Peoria, IL 61604, USA. kurtzman@mail.ncaur.usda.gov
authors.forename: C P
authors.initials: CP
authors.lastname: Kurtzman
authors.forename: C J
authors.initials: CJ
authors.lastname: Robnett
authors.forename: E
authors.initials: E
authors.lastname: Basehoar-Powers
mesh_terms: D004275:DNA, Ribosomal / Q000032:analysis; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013662:Tea / Q000382:microbiology*; D020068:Zygosaccharomyces / Q000145:classification* / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification
chemical_list: D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012337:RNA, Ribosomal, 18S; D013662:Tea; C027908:endodeoxyribonuclease DdeI; D015252:Deoxyribonucleases, Type II Site-Specific; C024405:GATC-specific type II deoxyribonucleases