The Entner-Doudoroff pathway has little effect on Helicobacter pylori colonization of mice.
Helicobacter pylori mutants deficient in 6-phosphogluconate dehydratase (6PGD) were constructed. Colonization densities were lower and minimum infectious doses were higher for mutant strains than for wild-type strains. In spite of better colonization, however, wild-type strains did not displace the mutant in cocolonization experiments. Loss of 6PGD diminishes the fitness of H. pylori in vivo, but the pathway is nonessential for colonization.
Wanken
Amy E
AE
Department of Veterinary Biosciences, Ohio State University, Columbus, Ohio 43210, USA.
Conway
Tyrrell
T
Eaton
Kathryn A
KA
D000818:Animals; D015169:Colony Count, Microbial; D005753:Gastric Mucosa / Q000382:microbiology*; D005942:Gluconates / Q000378:metabolism*; D006019:Glycolysis; D016480:Helicobacter pylori / Q000378:metabolism* / Q000472:pathogenicity; D006836:Hydro-Lyases / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL
D005942:Gluconates; C081217:2-keto-3-deoxy-6-phosphogluconate; D006836:Hydro-Lyases; C022362:phosphogluconate dehydratase; C008884:6-phosphogluconic acid