title: Construction and characterization of pentacistronic retrovirus vectors.
abstract: The picornavirus foot-and-mouth disease virus 2A sequence was combined with three different internal ribosome entry segments to construct and characterize three independent pentacistronic retroviruses of different sizes. Efficient co-expression of the five proteins was successful and titres obtained for these pentacistronic virus vectors (final genome size approximately 7.9 kb) were comparable to those of vector systems with shorter genomes. Other vectors constructed that exceeded the genome length of the wild-type virus suffered frequent deletions.
authors.affiliation: Departamento de Bioquímica y Biología Molecular-Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid, Facultad de Ciencias, Cantoblanco, 28049 Madrid, Spain.
authors.forename: Pablo de
authors.initials: P
authors.lastname: Felipe
authors.affiliation: Departamento de Bioquímica y Biología Molecular-Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid, Facultad de Ciencias, Cantoblanco, 28049 Madrid, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Izquierdo
mesh_terms: D016475:3T3 Cells; D000818:Animals; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics* / Q000378:metabolism; D017930:Genes, Reporter / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D012190:Retroviridae / Q000235:genetics*; D012270:Ribosomes / Q000235:genetics* / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D008164:Luminescent Proteins; D014764:Viral Proteins; D049452:Green Fluorescent Proteins