title: Eight-week histological analysis on the effect of chitosan on surgically created one-wall intrabony defects in beagle dogs.
abstract: OBJECTIVE
  To evaluate the periodontal tissue regenerative effects of a chitosan/collagen sponge applied to preclinical one-wall intrabony defects surgically created in beagle dogs.
  
  
  METHODS
  4 x 4 mm one-wall intrabony defects were surgically created in the bilateral maxillary first and third, and the mandibular second and fourth premolars. The surgical control group received a flap operation only, while the buffer control group was treated afterwards with a phosphate-buffered saline/collagen sponge (CS) and the chitosan group was treated with a chitosan/cs. The subjects were killed 8 weeks after the operation, and a comparative histological examination was performed.
  
  
  RESULTS
  The amount of junctional epithelium migration was 2.30+/-1.24 mm in the surgical control group, 1.49+/-1.25 mm in the buffer control group, and 0.26+/-0.59 mm in the chitosan group. A significant difference was exhibited only between the surgical control and the chitosan group (p<0.05). The amount of connective tissue adhesion was 0.68+/-0.60, 1.07+/-0.91, and 0.41+/-0.42 mm in the surgical control, buffer control, and the chitosan group, respectively. The amount of cementum regeneration was 1.42+/-0.49, 1.60+/-0.41, and 3.46+/-0.78 mm in the surgical control, buffer control, and the chitosan group, respectively. A significant difference was seen between the chitosan group and the rest (p<0.01). The amount of alveolar bone regeneration was 1.00+/-0.77, 1.52+/-0.37, and 2.43+/-0.44 mm in the surgical control, buffer control, and the chitosan group, respectively. A significant difference was observed between the chitosan group and the rest (p<0.05).
  
  
  CONCLUSIONS
  The results demonstrate the beneficial effect of the chitosan/cs on the one-wall intrabony defects of beagle dogs. The inhibited apical migration of epithelium and the increase in the amount of new bone and new cementum suggest the potency of chitosan in inducing periodontal tissue regeneration.
authors.affiliation: Department of Periodontology, Institute for Periodontal Regeneration, College of Dentistry, Yonsei University, Seoul, Korea.
authors.forename: Ji-Sook
authors.initials: JS
authors.lastname: Park
authors.forename: Seong-Ho
authors.initials: SH
authors.lastname: Choi
authors.forename: Ik-Sang
authors.initials: IS
authors.lastname: Moon
authors.forename: Kyoo-Sung
authors.initials: KS
authors.lastname: Cho
authors.forename: Jung-Kiu
authors.initials: JK
authors.lastname: Chai
authors.forename: Chong-Kwan
authors.initials: CK
authors.lastname: Kim
mesh_terms: D016301:Alveolar Bone Loss / Q000601:surgery*; D000539:Alveolar Process / Q000473:pathology*; D000818:Animals; D001704:Biopolymers / Q000627:therapeutic use*; D001861:Bone Regeneration / Q000187:drug effects; D018786:Bone Substitutes / Q000627:therapeutic use*; D002686:Chitin / Q000031:analogs & derivatives* / Q000627:therapeutic use*; D048271:Chitosan; D003094:Collagen / Q000627:therapeutic use; D003238:Connective Tissue / Q000473:pathology; D003739:Dental Cementum / Q000473:pathology; D004195:Disease Models, Animal; D004285:Dogs; D004846:Epithelial Attachment / Q000473:pathology; D008297:Male; D008334:Mandible / Q000601:surgery; D008437:Maxilla / Q000601:surgery; D012038:Regeneration / Q000187:drug effects; D018709:Statistics, Nonparametric; D013524:Surgical Flaps
chemical_list: D001704:Biopolymers; D018786:Bone Substitutes; D002686:Chitin; D003094:Collagen; D048271:Chitosan