title: Fatty acids as an energy source for the operation of axoplasmic transport.
abstract: Fatty acids are utilized as a cellular energy source. In the present study, we investigated whether fatty acids could affect axoplasmic transport. Cultured mouse superior cervical ganglion neurons were placed in the glucose-containing medium (145 mM NaCl, 5 mM KCl, 1 mM CaCl(2), 1 mM MgCl(2), 5 mM D-glucose, 10 mM Hepes, pH 7.3, 37 degrees C), and axoplasmic transport of particles in neurites was observed under video-enhanced contrast microscopy. A variety of fatty acids (acetate (C2), caproate (C6), caprylate (C8), caprate (C10), 2-decenoate (C10:1), arachidonate (C20:4); 0.1-1 mM) caused a transient increase in the amount of particles transported in both anterograde and retrograde directions. The increasing effects of fatty acids were dose-dependent. A half-maximum effective dose (ED(50)) for acetate was 0.8 mM, which is similar to the reported K(m) value of acetyl-CoA synthetase for acetate. The ED(50) for caprylate was 28 microM, which is near the K(m) value of acyl-CoA synthetase for medium- and long-chain fatty acids. Application of 5 mM malonate, an inhibitor of the citrate cycle, induced a steady-state decrease in axoplasmic transport, indicating that energy derived from the citrate cycle is required for the maintenance of axoplasmic transport. The increasing effect of acetate (1 mM) on axoplasmic transport was completely abolished by pretreatment with malonate (5 mM), suggesting that acetate produces ATP for axoplasmic transport via the citrate cycle. Alternatively, the effect of caprate (1 mM) was retained after treatment with malonate. Thus, fatty acids except acetate produce ATP probably through both the beta-oxidation pathway and the citrate cycle, increasing axoplasmic transport. Since the effect of fatty acids was transient, certain negative feedback mechanisms might be involved. The removal of glucose from the medium resulted in a low steady-state level of axoplasmic transport. Under such condition, the acetate (1 mM)-induced transient increase in axoplasmic transport remained. Since intracellular ATP must be low under glucose-free condition, intracellular ATP concentrations are unlikely to be involved in the feedback system. Instead, acetyl-CoA or its downstream products in the citrate cycle might lead to feedback inhibition. Application of citrate (5 mM) caused a strong decrease following a transient increase in axoplasmic transport, whereas no other acetyl-CoA product decreased axoplasmic transport. Thus, excessive citrate may be one of factors leading to feedback inhibition of metabolic pathways to arrest and reverse the increase in axoplasmic transport induced by fatty acids.
authors.affiliation: Department of Sport and Medical Science, Kokushikan University, 7-3-1 Nagayama, Tama-shi, Tokyo 206-8515, Japan. ttakenak@kokushikan.ac.jp
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Takenaka
authors.forename: Hiromi
authors.initials: H
authors.lastname: Hiruma
authors.forename: Hideaki
authors.initials: H
authors.lastname: Hori
authors.forename: Yoko
authors.initials: Y
authors.lastname: Hashimoto
authors.forename: Takafumi
authors.initials: T
authors.lastname: Ichikawa
authors.forename: Tadashi
authors.initials: T
authors.lastname: Kawakami
mesh_terms: D000818:Animals; D001370:Axonal Transport / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D002951:Citrates / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004791:Enzyme Inhibitors; D005227:Fatty Acids / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology; D007461:Iodoacetates / Q000494:pharmacology; D008314:Malonates / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D011837:Radiation-Protective Agents / Q000494:pharmacology; D019802:Succinic Acid / Q000494:pharmacology; D017783:Superior Cervical Ganglion / Q000502:physiology*; D013997:Time Factors
chemical_list: D002951:Citrates; D004791:Enzyme Inhibitors; D005227:Fatty Acids; D007461:Iodoacetates; D008314:Malonates; D011837:Radiation-Protective Agents; C030290:malonic acid; D019802:Succinic Acid; D005947:Glucose