[Intravital microscopic analysis of perfusion, leukocyte-endothelial cell interaction and neovascularization after burns: an in vivo study of SKH-1/hr hairless mice]].
Breakdown of skin microcirculation is supposed to play a key role in pathophysiology of burn injury. The aim of the study was to develop a burn model, which allows repetitive quantitative in vivo analysis of microcirculation after burn injury with special focus on leukocyte endothelium interaction over a longer period of time. Male hairless mice (SKH-1/hr) were used. Deep partial thickness burns were inflicted in no-touch-technique to the ears. Intravital fluorescent microscopy in combination with FITC-dextran as a plasma-marker was used to assess standard microcirculatory parameters. Leukocytes were stained with rhodamine-6-G to study their interaction with the endothelium.
Goertz
O
O
Klinik für Plastische Chirurgie und Schwerbrandverletzte-Handchirurgiezentrum, BG-Kliniken Bergmannsheil, Ruhr-Universität Bochum, Bürkle-de-la-Camp-Platz 1, 44789 Bochum. olegoertz@gmx.net
Langer
S
S
Homann
H H
HH
Steinstraesser
L
L
Steinau
H U
HU
D000818:Animals; D002056:Burns / Q000503:physiopathology*; D002450:Cell Communication / Q000502:physiology*; D004730:Endothelium, Vascular / Q000503:physiopathology*; D007962:Leukocytes / Q000502:physiology*; D008297:Male; D051379:Mice; D008812:Mice, Hairless; D008815:Mice, Inbred Strains; D008833:Microcirculation / Q000503:physiopathology; D008856:Microscopy, Fluorescence; D012867:Skin / Q000098:blood supply*
Intravitalmikroskopische Analyse von Perfusion, Leukozyten-Endothelzell-Interaktion und Neovaskularisation nach Verbrennung: Eine in vivo-Studie an SKH-1/hr Haarlosen Mäusen.