title: Interpretation of intraspecific variability in mtDNAs of Aspergillus niger strains and rearrangement of their mtDNAs following mitochondrial transmissions.
abstract: Physical and functional maps of mitochondrial DNAs of Aspergillus niger strains representing different mitochondrial DNA RFLP patterns were constructed and compared. In spite of the high similarity in the organisation of mitochondrial DNAs among examined strains, differences could be easily recognised by applying molecular markers, such as the different intron content of the cox1 genes, the sequence of the intergenic regions between the Met- and His-tRNA genes and downstream of the tRNA-Gly gene. Intraspecific mitochondrial transfers between the heterokaryon incompatible mitochondrial oligomycin-resistant A. niger strain, as the donor, and other A. niger-sensitive strains bearing different RFLP patterns resulted in oligomycin-resistant progeny possessing either rearranged or unchanged donor mitochondrial DNA and recipient nuclei. Since the intergenic marker sequences of mitochondrial DNAs turned out to be identical in the donor and the progeny, it can be assumed that the oligomycin-resistant progeny inherit the mitochondrial DNA of the donor strain; this may either remain unchanged or may be modified by a mobile intron of the cox1 gene of the recipient mitochondria.
authors.affiliation: Department of Microbiology, Faculty of Sciences, University of Szeged, H-6701 Szeged, P.O. Box 533, Hungary.
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Hamari
authors.forename: Beáta
authors.initials: B
authors.lastname: Tóth
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Beer
authors.forename: Attila
authors.initials: A
authors.lastname: Gácser
authors.forename: Judit
authors.initials: J
authors.lastname: Kucsera
authors.forename: Ilona
authors.initials: I
authors.lastname: Pfeiffer
authors.forename: Akos
authors.initials: A
authors.lastname: Juhász
authors.forename: Ferenc
authors.initials: F
authors.lastname: Kevei
mesh_terms: D001234:Aspergillus niger / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D001483:Base Sequence; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D004271:DNA, Fungal / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D014644:Genetic Variation; D008928:Mitochondria / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D012150:Polymorphism, Restriction Fragment Length; D011995:Recombination, Genetic; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal; D004272:DNA, Mitochondrial