title: Clusters of chromosomal imbalances in thymic epithelial tumours are associated with the WHO classification and the staging system according to Masaoka.
abstract: Using comparative genomic hybridisation, we investigated chromosomal imbalances in 28 cases of thymic epithelial neoplasms including type A, B2, B3, the A component of type AB and different subtypes of type C thymoma. To identify different patterns of chromosomal aberrations associated with the biological behaviour and the histological diversity of thymomas, a hierarchical cluster analysis of 65 cases was performed. The here-reported Comparative Genomic Hybridisation (CGH) data (28 cases) of partly uninvestigated tumour subtypes were pooled with previously published data of chromosomal imbalances of 37 thymomas (Zettl et al. [Am J Pathol 2000;157:257-66]). The analysis of 278 chromosomal subbands yielded 2 main clusters. The first main cluster was characterised by gains of the chromosomal arm 1q, consisted only of type C and B3 thymomas and was further subdivided into 2 subgroups. To the first subgroup only thymomas were attributed, which, in addition to gains of the chromosomal arm 1q, showed losses on 6q and 16q, whereas tumours belonging to the second subgroup exhibited no further recurrent chromosomal alterations. The second main cluster was formed by a heterogeneous group of thymoma types (types A, AB, B2, B3 and C), showing no specific pattern of chromosomal imbalances. In 19 thymomas, no chromosomal imbalances could be detected (3 type B2 and 5 type A thymomas of our study as well as 11 type A thymomas investigated by Zettl et al., Am J Pathol 2000;157:257-66). Chromosomal imbalances were more frequent in type C thymomas than in other subtypes. The distribution of tumour stages according to Masaoka (p = 0.003) and the World Health Organisation (WHO) classification (p < 0.0001) was significantly different in the clusters and subgroups obtained. The groups reflect the staging system and the WHO classification and show that type B3 and type C carcinomas have a strong relationship concerning their chromosomal imbalances. Furthermore, chromosomal imbalances detected in some type A thymomas might be responsible for the aggressive behaviour described in a few cases of this thymoma subtype.
authors.affiliation: Department of Pathology, University of Heidelberg, Heidelberg, Germany.
authors.forename: Roland
authors.initials: R
authors.lastname: Penzel
authors.forename: Josef
authors.initials: J
authors.lastname: Hoegel
authors.forename: Waltraud
authors.initials: W
authors.lastname: Schmitz
authors.forename: Hendrik
authors.initials: H
authors.lastname: Blaeker
authors.forename: Alicia
authors.initials: A
authors.lastname: Morresi-Hauf
authors.forename: Sebastian
authors.initials: S
authors.lastname: Aulmann
authors.forename: Erich
authors.initials: E
authors.lastname: Hecker
authors.forename: Gunhild
authors.initials: G
authors.lastname: Mechtersheimer
authors.forename: Herwart F
authors.initials: HF
authors.lastname: Otto
authors.forename: Ralf J
authors.initials: RJ
authors.lastname: Rieker
mesh_terms: D000328:Adult; D000368:Aged; D002869:Chromosome Aberrations; D016000:Cluster Analysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D013945:Thymoma / Q000145:classification / Q000235:genetics* / Q000473:pathology*; D013953:Thymus Neoplasms / Q000145:classification / Q000235:genetics* / Q000473:pathology*