[Isolation, purification, and properties of factor XII and its active fragment (beta-XIIa)].
A procedure of isolation of human blood plasma prekallikrein, coagulation factor XII and active fragment beta-XIIa has been developed. This procedure includes the traditional chromatography steps and FPLC. Disc-electrophoresis revealed that the preparations of factor XII and beta-XIIa were homogeneous. Their specific activity was 70.6 U and 2.5 U, respectively. The procedure described is less time consuming and it allows to isolate these factors in the preparative quantities.
Blokhina
T B
TB
Russian Medical Academy of Postgraduate Education, Barrikadnaya st., 2, Moscow, 123995 Russia.
Neshkova
E A
EA
Iarovaia
G A
GA
D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005174:Factor XII / Q000737:chemistry* / Q000302:isolation & purification*; D006801:Humans
D005174:Factor XII
Poluchenie, ochistka, svoĭstva faktora XII i ego aktivnogo fragmenta (beta-XIIa).