Antisense oligonucleotide against collagen-specific molecular chaperone 47-kDa heat shock protein suppresses scar formation in rat wounds.
The 47-kDa heat shock protein (HSP47) is a molecular chaperone specifically targeting the processing and quality control of collagen molecules. This study was performed to investigate whether antisense therapy preventing HSP47 expression might affect the scar formation occurring during wound healing of skin. In wound healing of neonatal rat skin, the number of HSP47-positive cells and the amount of HSP47 protein consistently increased up to 7 days after surgical wounding. The increase in HSP47-positive cell number and protein content was efficiently suppressed by daily injections of HSP47-antisense deoxynucleotide (30 nmol) for 7 days. This treatment also suppressed the accumulation of collagen type I in the wound. Moreover, the disorder of collagenous fibers was relieved in the healed portion of the wounds subjected to the antisense treatment. Taken together, the authors propose that HSP47 is an important determinant in scar formation and that the antisense treatment against HSP47 gene may have a therapeutic potential to suppress the scar formation of skin.
Wang
Zuolin
Z
Second Department of Oral and Maxilofacial Surgery, Nagasaki University School of Dentistry, Nasagaki, Japan. zuolin@net.nagasaki-u.ac.jp
Inokuchi
Tsugio
T
Nemoto
Takayuki K
TK
Uehara
Masataka
M
Baba
Tomomi T
TT
D000818:Animals; D000831:Animals, Newborn; D002921:Cicatrix / Q000503:physiopathology / Q000517:prevention & control*; D003094:Collagen / Q000378:metabolism; D050958:HSP47 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000502:physiology; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000293:injuries* / Q000378:metabolism / Q000503:physiopathology; D014945:Wound Healing / Q000502:physiology*
D050958:HSP47 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D016376:Oligonucleotides, Antisense; C494662:Serpinh1 protein, rat; D003094:Collagen