Performance of a recombinant strain of Streptomyces lividans for bioconversion of penicillin G to deacetoxycephalosporin G.
We examined the performance of Streptomyces lividans strain W25 containing a hybrid expandase (deacetoxycephalosporin C synthase; DAOCS) gene, obtained by in vivo recombination between the expandase genes of S. clavuligerus and Nocardia lactamdurans for resting-cell bioconversion of penicillin G to deacetoxycephalosporin G. Strain W25 carried out a much more effective level of bioconversion than the previously used strain, S. clavuligerus NP1. The two strains also differed in the concentrations of FeSO(4) and alpha-ketoglutarate giving maximal activity. Whereas NP1 preferred 1.8 mM FeSO(4 )and 1.3 mM alpha-ketoglutarate, recombinant W25 performed best at 0.45 mM FeSO(4) and 1.9 mM alpha-ketoglutarate.
Gao
Q
Q
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Piret
J M
JM
Adrio
J L
JL
Demain
A L
AL
D001711:Biotransformation; D002511:Cephalosporins / Q000096:biosynthesis* / Q000378:metabolism; D003470:Culture Media; D005296:Ferrous Compounds / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D019751:Intramolecular Transferases / Q000235:genetics / Q000378:metabolism*; D007656:Ketoglutaric Acids; D010400:Penicillin G / Q000378:metabolism*; D046915:Penicillin-Binding Proteins; D013302:Streptomyces / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D013696:Temperature
D002511:Cephalosporins; D003470:Culture Media; D005296:Ferrous Compounds; D007656:Ketoglutaric Acids; D046915:Penicillin-Binding Proteins; C405006:deacetoxycephalosporin G; C020748:ferrous sulfate; D019751:Intramolecular Transferases; C038371:deacetoxycephalosporin C synthetase; D010400:Penicillin G