New approaches to augment fungal biotransformation.
The possibility of using solid supports and intermittent substrate feeding to manipulate biotransformation by fungi was examined, with amoxapine as a model compound. Cunninghamella elegans ATCC 8688a grown as free cells in six-well plates showed 7-hydroxyamoxapine as the major metabolite of amoxapine biotransformation. However, when cells were grown in the presence of activated carbon, N-formyl-7-hydroxyamoxapine was formed as the major metabolite. Intermittent feeding of amoxapine also favored the formation of N-formyl-7-hydroxyamoxapine.
Shanmugam
Balajee
B
Natural Products Microbiology, Wyeth Research, 401 N. Middletown Road, Pearl River, NY 10965, USA. shanmub@wyeth.com
Luckman
Scott
S
Summers
Mia
M
Bernan
Valerie
V
Greenstein
Michael
M
D000657:Amoxapine / Q000031:analogs & derivatives / Q000378:metabolism*; D018687:Antidepressive Agents, Second-Generation / Q000378:metabolism; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D020093:Cunninghamella / Q000254:growth & development / Q000378:metabolism*; D013058:Mass Spectrometry
D018687:Antidepressive Agents, Second-Generation; C478364:N-formyl-7-hydroxyamoxapine; D000657:Amoxapine