FR252921, a novel immunosuppressive agent isolated from Pseudomonas fluorescens no. 408813. I. Taxonomy, fermentation, isolation, physico-chemical properties and biological activities of FR252921, FR252922 and FR256523.
Novel immunosuppressive agents, FR252921, FR252922 and FR256523 were isolated from the cultured broth of a bacterial strain No. 408813. The strain was identified Pseudomonas fluorescens from morphological and physiological characteristics. FR252921, FR252922 and FR256523, novel compounds containing macrolactone ring, showed immunosuppressive activity against murine splenocyte proliferation stimulated with lipopolysaccharide (LPS) or anti-CD3 mAb in vitro.
Fujine
Kiyotaka
K
Exploratory Research Laboratories, Fujisawa Pharmaceutical Co., Ltd., 5-2-3 Tokodai, Tsukuba, Ibaraki 300-2698, Japan. kiyotaka_fujine@po.fujisawa.co.jp
Tanaka
Miho
M
Ohsumi
Keisuke
K
Hashimoto
Michizane
M
Takase
Shigehiro
S
Ueda
Hirotsugu
H
Hino
Motohiro
M
Fujii
Takashi
T
D000818:Animals; D002455:Cell Division / Q000187:drug effects / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D005260:Female; D005285:Fermentation; D007166:Immunosuppressive Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D007783:Lactones / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D008970:Molecular Weight; D019906:Nuclear Magnetic Resonance, Biomolecular; D009904:Optical Rotation; D011551:Pseudomonas fluorescens / Q000737:chemistry / Q000145:classification / Q000378:metabolism* / Q000502:physiology; D012988:Soil Microbiology; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology
D007166:Immunosuppressive Agents; D007783:Lactones