title: Analysis of voluntary blood donors with biologic false reactivity on chemiluminescent immunoassays and implications for donor management.
abstract: BACKGROUND
  Biologic false-reactive (BFR) results in blood donors are problematic due to both component loss and donor-management issues. This report analyzes the results of a longitudinal study of BFR donors and the implications for donor management.
  
  
  METHODS
  Donors who gave BFR results on HBsAg, HIV-1/HIV-2, HCV, or HTLV-I/HTLV/II chemiluminescent immunoassays (ChLIAs) (PRISM, Abbott) between May 1997 to March 1999 were analyzed. Donors were followed up for up to three donations after an index BFR episode. In addition, results of any negative donations before the index BFR result but within the study period were included in the analysis.
  
  
  RESULTS
  For donors who gave an index BFR result on the HBsAg ChLIA, 14.3 percent remained BFR at subsequent donations, whereas for the anti-HIV-1/HIV-2, anti-HCV, and anti-HTLV-I/HTLV-II ChLIAs, the figures were 66.0, 77.4, and 71.6 percent, respectively. For donors who gave a second BFR result, the percentage who remained BFR at subsequent donations was 75.0, 80.6, 84.6, and 74.5 percent for the four assays, respectively. The rate at which negative repeat donors became BFR during the study period was 0.02, 0.07, 0.12, and 0.02 percent for the HBsAg, anti-HIV-1/HIV-2, anti-HCV, and anti-HTLV-I/HTLV-II assays, respectively.
  
  
  CONCLUSIONS
  Our results indicate that donors who give an index BFR result on the ChLIAs (PRISM, Abbott) should be allowed to continue donating because most donors with a HBsAg BFR result were negative at subsequent donations, and between 22.6 and 34.0 percent of those with BFR results on the HIV-1/HIV-2, HCV, or HTLV-I/HTLV-II assays gave subsequent negative donations. However, donors who give a second BFR result should be counseled and deferred because they were very unlikely to give subsequent negative results.
authors.affiliation: Virus Serology Unit, Australian Red Cross Blood Service-Victoria, PO Box 354, South Melbourne, Victoria 3205, Australia. pkiely@arcbs.redcross.org.au
authors.forename: Philip
authors.initials: P
authors.lastname: Kiely
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Stewart
authors.forename: Lea
authors.initials: L
authors.lastname: Castro
mesh_terms: D001769:Blood / Q000821:virology; D001782:Blood Donors; D005189:False Positive Reactions; D006678:HIV / Q000302:isolation & purification; D006403:Hematologic Tests; D016174:Hepacivirus / Q000302:isolation & purification; D006509:Hepatitis B / Q000517:prevention & control; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D015368:Human T-lymphotropic virus 1 / Q000302:isolation & purification; D015367:Human T-lymphotropic virus 2 / Q000302:isolation & purification; D006801:Humans; D007118:Immunoassay; D008137:Longitudinal Studies; D008163:Luminescent Measurements; D014611:Vaccination / Q000706:statistics & numerical data
chemical_list: D006514:Hepatitis B Surface Antigens