SNP haplotype tagging from DNA pools of two individuals.
BACKGROUND
DNA pooling is a technique to reduce genotyping effort while incurring only minor losses in accuracy of allele frequency estimates for single nucleotide polymorphism (SNP) markers.


RESULTS
We present an algorithm for reconstructing haplotypes (alleles for multiple SNPs on same chromosome) from pools of two individual DNAs, in which Hardy-Weinberg equilibrium conditions or other assumptions are not required. The program outputs, in addition to inferred haplotypes, a minimal number of haplotype-tagging SNPs that are identified after an exhaustive search procedure.


CONCLUSIONS
Our method and algorithms lead to a significant reduction in genotyping effort, for example, in case-control disease association studies while maintaining the possibility of reconstructing haplotypes under very general conditions.
Hoh
Josephine
J
Laboratory of Statistical Genetics, Rockefeller University, New York, NY 10021, USA. jhoh@linkage.rockefeller.edu
Matsuda
Fumihiko
F
Peng
Xu
X
Markovic
Daniela
D
Lathrop
Mark G
MG
Ott
Jurg
J
D000465:Algorithms; D000483:Alleles; D002148:Calmodulin-Binding Proteins / Q000235:genetics; D019295:Computational Biology / Q000379:methods; D003598:Cytoskeletal Proteins / Q000235:genetics; D004247:DNA / Q000032:analysis*; D005838:Genotype; D006239:Haplotypes / Q000235:genetics*; D006801:Humans; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D000099291:Adducins
D002148:Calmodulin-Binding Proteins; D003598:Cytoskeletal Proteins; D004247:DNA; D000099291:Adducins