Establishment of an R-banded rabbit karyotype nomenclature by FISH localization of 23 chromosome-specific genes on both G- and R-banded chromosomes.
Direct detection of fluorescent in situ hybridization signals on R-banded chromosomes stained with propidium iodide is a rapid and efficient method for constructing cytogenetic maps for species with R-banded standard karyotypes. In this paper, our aim is to establish an R-banded rabbit karyotype nomenclature that is in total agreement with the 1981 G-banded standard nomenclature. For this purpose, we have produced new GTG- and RBG-banded mid-metaphase karyotypes and an updated version of ideograms of R-banded rabbit chromosomes. In addition, to confirm correlations between G- and R-banded chromosomes, we have defined a set of 23 rabbit BAC clones, each containing a specific gene, one marker gene per rabbit chromosome, and we have localized precisely each BAC clone by FISH on both G- and R-banded chromosomes.
Hayes
H
H
Laboratoire de Génétique biochimique et Cytogénétique, INRA, Jouy-en-Josas, France. hayes@diamant.jouy.inra.fr
Rogel-Gaillard
C
C
Zijlstra
C
C
De Haan
N A
NA
Urien
C
C
Bourgeaux
N
N
Bertaud
M
M
Bosma
A A
AA
D000818:Animals; D002871:Chromosome Banding; D002874:Chromosome Mapping; D033481:Chromosomes, Mammalian / Q000648:ultrastructure; D005796:Genes; D005819:Genetic Markers; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D011817:Rabbits / Q000235:genetics*; D009626:Terminology as Topic
D005819:Genetic Markers