title: [IgM-ELISA for diagnosis of schistosomiasis mansoni in low endemic areas].
abstract: An immunoenzymatic method for the detection of IgM antibodies (IgM-ELISA) against a fraction of Schistosoma mansoni adult worm antigen, soluble in trichloroacetic acid (TCA-soluble fraction), was evaluated for epidemiological purposes in low endemic areas for schistosomiasis. Blood samples on filter paper were collected from a population living in the municipality of Pedro de Toledo, São Paulo State, and submitted to IgM-ELISA. The results were compared to those obtained by the IgM-immunofluorescence test (IgM-IFT) and the Kato-Katz parasitological method. Positive rates of 36.8%, 33.5%, and 1.6% were obtained respectively by the IgM-ELISA, IgM-IFT, and Kato-Katz methods. The geometric mean obtained by the parasitological method was 40.9 eggs per gram of feces (epg). The nearly perfect agreement observed between the two serological tests (Kappa index of 0,89) indicates good diagnostic performance by the evaluated test. IgM-ELISA is a potentially useful method for diagnosis of schistosomiasis in individuals with low worm burden.
authors.affiliation: Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, SP, 05508-900, Brasil. edwardoliveira@bol.com.br
authors.forename: Edward José
authors.initials: EJ
authors.lastname: de Oliveira
authors.forename: Hermínia Yoshio
authors.initials: HY
authors.lastname: Kanamura
authors.forename: Luiz Cândido de Souza
authors.initials: LC
authors.lastname: Dias
authors.forename: Lanny Cristina Burlandy
authors.initials: LC
authors.lastname: Soares
authors.forename: Dirce Mary Correia
authors.initials: DM
authors.lastname: Lima
authors.forename: Ricardo Mário de Carvalho
authors.initials: RM
authors.lastname: Ciaravolho
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood* / Q000302:isolation & purification; D019353:Endemic Diseases; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005243:Feces / Q000469:parasitology; D005260:Female; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood* / Q000276:immunology; D008297:Male; D010270:Parasite Egg Count; D012550:Schistosoma mansoni / Q000276:immunology; D012555:Schistosomiasis mansoni / Q000097:blood / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; D007075:Immunoglobulin M
vernacular_title: ELISA-IgM para diagnóstico da esquistossomose mansoni em área de baixa endemicidade.