Identification of a novel allele HLA-B*5610 in a Chinese potential bone marrow donor.
A novel HLA-B allele, B*5610, has been identified in a potential bone marrow donor, his mother and brother using DNA-based typing and molecular cloning methods. The B*5610 allele differs from the closest matching HLA sequence of B*5602 by two nucleotide substitutions in exon 3, 559 C-->A and 560 T-->C, resulting in an amino acid change from Leu (CTG) to Thr (ACG) at codon 187. This new allele was segregated together with A*24020101 and DRB1*140101 in the proband's family. Serology study revealed that B*5610 is associated with B22 specificity. A PCR-SSP method was developed to distinguish B*5610 from other B*56 alleles. No further individuals with B*5610 were detected in 5000 Chinese bone marrow blood donors.
Wu
G-G
GG
Shenzhen Institute of Transfusion Medicine, Shenzhen, China.
Cheng
L-H
LH
Zhou
D
D
Deng
Z-H
ZH
Zou
H-Y
HY
Wei
T-L
TL
Li
Z
Z
Li
D-C
DC
Gao
S-Q
SQ
Zhao
T-M
TM
D000483:Alleles; D001483:Base Sequence; D002681:China; D003001:Cloning, Molecular; D005787:Gene Frequency; D015235:HLA-B Antigens / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D015235:HLA-B Antigens