Granulocyte colony stimulating factor does not induce long-term DNA instability in healthy peripheral blood stem cell donors.
To establish the safety of using G-CSF in healthy PBSC donors, we prospectively determined the degree of DNA destabilization in peripheral blood WBC. Donors were treated with SC G-CSF for 5 days. A baseline sample of peripheral blood was collected before G-CSF treatment and consecutive samples were collected on day 5, 1 month, and 2 months after treatment. The extent of double-stranded DNA relaxation and de novo synthesis of DNA was determined in each sample. We found that both parameters of DNA destabilization were significantly increased after 5 days of G-CSF and returned to baseline level at 1 and 2 months. We assume that these findings suggest that rhG-CSF administration to healthy PBSCT donors for 5 days may be a safe procedure.
Shapira
Michael Y
MY
Department of Bone Marrow Transplantation, Hadassah-Hebrew University Hospital, PO Box 12000, Jerusalem, Israel 91120. shapiram@hadassah.org.il
Kaspler
Pavel
P
Samuel
Simcha
S
Shoshan
Shmuel
S
Or
Reuven
R
D001782:Blood Donors; D004247:DNA / Q000096:biosynthesis / Q000097:blood* / Q000737:chemistry; D004355:Drug Stability; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage / Q000009:adverse effects*; D006412:Hematopoietic Stem Cells; D006801:Humans; D007958:Leukocyte Count; D007962:Leukocytes / Q000737:chemistry* / Q000378:metabolism; D036102:Peripheral Blood Stem Cell Transplantation; D011446:Prospective Studies; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000009:adverse effects
D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D004247:DNA