title: [Expression patterns in isoforms of vascular endothelial growth-factors in tissue samples of vulval cancer T1 N2M0 stage].
abstract: OBJECTIVE
  The aim of the study was estimating the expression of isoforms of mRNA vascular endothelial growth factor: VEGF121, VEGF145, VEGF165, VEGF183, VEGF189, VEGF206, in tissue samples of vulvar cancer, normal and inguinal lymph nodes metastases.
  
  
  METHODS
  The material for this investigation of expression of mRNA selected genes was 4 tissue specimens from women with vulvar cancer T1N2M0. The method used for quantitative determination of the numbers of mRNA copies of selected genes was the QRT-PCR and QPCR technique, with the use of an ABI PRISM 7700 (TaqMan) sequence detector. The nucleotide sequence for starters and probes for quantitative RT-PCR was designed with the use of the Primer Express Version 1.0 ABI PRISM software.
  
  
  RESULTS
  Expression of all mRNA isoforms VEGF was found in the samples. The number of mRNA copies obtained in 1 microgram total mRNA extract of the genes examined in tissue sections with various pathomorphology were clearly differentiated.
  
  
  CONCLUSIONS
  Expression profile of aforementioned genes indicated a definitive relationship between vascular endothelial growth factor isoform and vulvar cancer. These findings provide evidence that the expression pattern of mRNA VEGF isoform should find broad applications in clinical and research settings.
authors.affiliation: Katedry i Oddziału Klinicznego Połoznictwa i Ginekologii SAM, Tychach.
authors.forename: Bogdan
authors.initials: B
authors.lastname: Michalski
authors.forename: Urszula
authors.initials: U
authors.lastname: Mazurek
authors.forename: Anita
authors.initials: A
authors.lastname: Olejek
authors.forename: Tomasz
authors.initials: T
authors.lastname: Loch
authors.forename: Michał
authors.initials: M
authors.lastname: Graniczka
authors.forename: Ryszard
authors.initials: R
authors.lastname: Poreba
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Wilczok
mesh_terms: D016228:Endothelial Growth Factors / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis* / Q000235:genetics; D008222:Lymphokines / Q000096:biosynthesis* / Q000235:genetics; D009367:Neoplasm Staging; D020033:Protein Isoforms / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D014846:Vulvar Neoplasms / Q000737:chemistry* / Q000473:pathology*
chemical_list: D016228:Endothelial Growth Factors; D036341:Intercellular Signaling Peptides and Proteins; D008222:Lymphokines; D020033:Protein Isoforms; D012333:RNA, Messenger; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
vernacular_title: Wzory ekspresji izoform naczyniowo-śródbłonkowego czynnika wzrostu (VEGF) w raku sromu T1N2M0.