title: Neurotrophin-3 specifically increases mature oligodendrocyte population and enhances remyelination after chemical demyelination of adult rat CNS.
abstract: In human central nervous system (CNS) demyelinating diseases, spontaneous remyelination is often incomplete. Therefore, we have tested whether neutrotrophin-3 (NT-3) accelerates CNS myelin repair after a chemically-induced demyelination. One group of adult rats was injected in the corpus callosum (CC) with 1 microl of 1% lysophosphatidylcholine (LPC) and 1 microl of NT-3 (1 microg/microl), and 15 days after injury (D15) remyelination was compared to control rats (receiving 1 microl of LPC+1 microl of vehicle buffer of NT-3). The demyelinated volume decreased by 56% in NT-3-treated rats at D15, and immunohistochemistry showed an increase in mature MBP(+) oligodendrocytes (OL) (+66%) in treated animals (whereas less mature (CNP(+)) OL were unchanged). Since less than 3% axons degenerate in this model, and as astrocytic gliosis was not modified, these data suggest that NT-3 acts directly on cells of the OL lineage to enhance remyelination in vivo.
authors.affiliation: Cell Biology Laboratory, UPRES EA 3143, University Hospital, 4 rue Larrey, F 49033 Angers cedex 01, France. isabelle.jean@med.univ-angers.fr
authors.forename: Isabelle
authors.initials: I
authors.lastname: Jean
authors.forename: Céline
authors.initials: C
authors.lastname: Lavialle
authors.forename: Annick
authors.initials: A
authors.lastname: Barthelaix-Pouplard
authors.forename: Catherine
authors.initials: C
authors.lastname: Fressinaud
mesh_terms: D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000378:metabolism; D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002493:Central Nervous System Diseases / Q000473:pathology / Q000503:physiopathology; D003337:Corpus Callosum / Q000187:drug effects / Q000473:pathology; D003711:Demyelinating Diseases / Q000139:chemically induced / Q000473:pathology* / Q000503:physiopathology; D004195:Disease Models, Animal; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007150:Immunohistochemistry; D008244:Lysophosphatidylcholines / Q000633:toxicity; D008297:Male; D004676:Myelin Basic Protein / Q000378:metabolism; D009416:Nerve Regeneration / Q000502:physiology*; D020933:Neurotrophin 3 / Q000494:pharmacology*; D009836:Oligodendroglia / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013234:Stem Cells / Q000378:metabolism; D013997:Time Factors
chemical_list: D005904:Glial Fibrillary Acidic Protein; D008244:Lysophosphatidylcholines; D004676:Myelin Basic Protein; D020933:Neurotrophin 3; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases; D001973:Bromodeoxyuridine