Shewanella fidelis sp. nov., isolated from sediments and sea water.
Two marine bacterial strains, KMM 3582T and KMM 3589, isolated respectively from sediments of the South China Sea and sea water of the Sea of Japan, have been characterized. Comparative 16S rDNA sequence-based phylogenetic analysis placed the two strains in a separate branch of the gamma-Proteobacteria within the members of the genus Shewanella. KMM 3582T showed the highest similarity (97.1 and 97.4%, respectively) to Shewanella pealeana and Shewanella gelidimarina. The G + C contents of the DNAs of the two strains studied were 45.0 mol%. The level of DNA-DNA relatedness between the two strains was 82%, indicating that they represent a single genospecies. These organisms were slightly pinkish, Gram-negative, polarly flagellated, facultatively anaerobic, mesophilic (with temperature range from 4 to 30 degrees C), neutrophilic and haemolytic and were able to degrade alginate, gelatin and DNA. The novel organisms were susceptible to gentamicin, lincomycin, oleandomycin, streptomycin and polymyxin. The predominant fatty acids were characteristic for shewanellae: 13 : 0-i, 15 : 0-i, 16 : 0 and 16 : 1omega7. Eicosapentaenoic acid, 20 : 5omega3, was not detected. Phylogenetic evidence, together with phenotypic characteristics, showed that the two bacteria constitute a novel species of the genus Shewanella. The name Shewanella fidelis sp. nov. is proposed, with the type strain KMM 3582T (=LMG 20551T =ATCC BAA-318T).
Ivanova
Elena P
EP
Industrial Research Institute, Swinburne University of Technology, PO Box 218, Hawthorn, Vic 3122, Australia.|Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Pr. 100 Let Vladivostoku 159, Russia.
Sawabe
Tomoo
T
Laboratory of Microbiology, Graduate School of Fisheries Sciences, Faculty of Fisheries, Hokkaido University, 3-1-1 Minato-cho, Hakodate 041-8611, Japan.
Hayashi
Karin
K
Laboratory of Microbiology, Graduate School of Fisheries Sciences, Faculty of Fisheries, Hokkaido University, 3-1-1 Minato-cho, Hakodate 041-8611, Japan.
Gorshkova
Nataliya M
NM
Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Pr. 100 Let Vladivostoku 159, Russia.
Zhukova
Natalia V
NV
Institute of Marine Biology of the Far-Eastern Branch of the Russian Academy of Sciences, 690041 Vladivostok, Russia.
Nedashkovskaya
Olga I
OI
Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Pr. 100 Let Vladivostoku 159, Russia.
Mikhailov
Valery V
VV
Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Pr. 100 Let Vladivostoku 159, Russia.
Nicolau
Dan V
DV
Industrial Research Institute, Swinburne University of Technology, PO Box 218, Hawthorn, Vic 3122, Australia.
Christen
Richard
R
UMR 6078 CNRS and Université Nice Sophia-Antipolis, Bat. J. Maetz, F-06238 Villefranche sur mer cedex, France.
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001482:Base Composition; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D020592:Shewanella / Q000145:classification* / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*
D000900:Anti-Bacterial Agents; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S