title: Cloning and characterization of a novel RING-B-box-coiled-coil protein with apoptotic function.
abstract: We have identified a novel RING-B-box-coiled-coil (RBCC) protein (MAIR for macrophage-derived apoptosis-inducing RBCC protein) that consists of an N-terminal RING finger, followed by a B-box zinc finger, a coiled-coil domain, and a B30.2 domain. MAIR mRNA was expressed widely in mouse tissues and was induced by macrophage colony-stimulating factor in murine peritoneal and bone marrow macrophages. MAIR protein initially showed a granular distribution predominantly in the cytoplasm. The addition of zinc to transfectants containing MAIR cDNA as part of a heavy metal-inducible vector caused apoptosis of the cells characterized by cell fragmentation; a reduction in mitochondrial membrane potential; activation of caspase-7, -8, and -9, but not caspase-3; and DNA degradation. We also found that the RING finger and coiled-coil domains were required for MAIR activity by analysis with deletion mutants.
authors.affiliation: Third Department of Internal Medicine, National Defense Medical College, Namiki, Tokorozawa, Saitama 359-8513, Japan.
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Kimura
authors.forename: Shinya
authors.initials: S
authors.lastname: Suzu
authors.forename: Yukitsugu
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Nakata
authors.forename: Muneo
authors.initials: M
authors.lastname: Yamada
authors.forename: Naruo
authors.initials: N
authors.lastname: Kuwada
authors.forename: Takuya
authors.initials: T
authors.lastname: Matsumura
authors.forename: Takuya
authors.initials: T
authors.lastname: Yamashita
authors.forename: Takashi
authors.initials: T
authors.lastname: Ikeda
authors.forename: Ken
authors.initials: K
authors.lastname: Sato
authors.forename: Kazuo
authors.initials: K
authors.lastname: Motoyoshi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D051017:Apoptosis Regulatory Proteins; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D009928:Organ Specificity; D011506:Proteins / Q000235:genetics*; D016415:Sequence Alignment; D016335:Zinc Fingers
chemical_list: D051017:Apoptosis Regulatory Proteins; D002352:Carrier Proteins; D011506:Proteins; C476418:TRIM35 protein, human