Ramlibacter tataouinensis gen. nov., sp. nov., and Ramlibacter henchirensis sp. nov., cyst-producing bacteria isolated from subdesert soil in Tunisia.
Ramlibacter gen. nov. is proposed for two aerobic, chemo-organotrophic, cyst-producing soil bacterial strains. These bacteria are Gram-negative, non-flagellated rods or cysts, isolated from subdesert soil in Tataouine, Tunisia. Phylogenetic analyses of the rrs sequences of the two strains showed that they do not constitute a robust clade at the genus level with any previously described bacteria and that they are a deep branch of a clade also grouping the genera Acidovorax and Hydrogenophaga within the beta-Proteobacteria. They belong to two different species, as verified by DNA-DNA hybridization (23.5% reassociation). The type species of the genus is Ramlibacter tataouinensis sp. nov., with the type strain TTB310T (=DSM 14655T =ATCC BAA-407T =LMG 21543T). The second species is Ramlibacter henchirensis sp. nov., with the type strain TMB834T (=DSM 14656T =ATCC BAA-408T =LMG 21542T). The G + C contents of R. tataouinensis and R. henchirensis are 69.6 and 66.6 mol%, respectively.
Heulin
Thierry
T
CEA/Cadarache, DSV-DEVM, Laboratoire d'Ecologie Microbienne de la Rhizosphère UMR 163 CNRS-CEA-Univ. Méditerranée, F-13108 Saint-Paul-lez-Durance, France.
Barakat
Mohamed
M
CEA/Cadarache, DSV-DEVM, Laboratoire d'Ecologie Microbienne de la Rhizosphère UMR 163 CNRS-CEA-Univ. Méditerranée, F-13108 Saint-Paul-lez-Durance, France.
Christen
Richard
R
UMR 6078 CNRS-Université de Nice Sophia-Antipolis, Bât. Jean Maetz, F-06230 Villefranche-sur-mer, France.
Lesourd
Maurice
M
Service Commun de Microscopie Electronique, CNRS & Université d'Angers, rue Haute de Reculée, F-49045 Angers, France.
Sutra
Laurent
L
UMR 077 de Pathologie Végétale, INRA-INH-Université d'Angers, 42 rue Georges-Morel, BP 57, F-49071 Beaucouzé Cedex, France.
De Luca
Gilles
G
CEA/Cadarache, DSV-DEVM, Laboratoire d'Ecologie Microbienne de la Rhizosphère UMR 163 CNRS-CEA-Univ. Méditerranée, F-13108 Saint-Paul-lez-Durance, France.
Achouak
Wafa
W
CEA/Cadarache, DSV-DEVM, Laboratoire d'Ecologie Microbienne de la Rhizosphère UMR 163 CNRS-CEA-Univ. Méditerranée, F-13108 Saint-Paul-lez-Durance, France.
D020562:Betaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008858:Microscopy, Phase-Contrast; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012988:Soil Microbiology; D014416:Tunisia
D004269:DNA, Bacterial; D005227:Fatty Acids