Identification of plu genes and cis-acting elements of PCNA in the Drosophila genus using conservation of gene order.
In Drosophila melanogaster, the cell cycle control gene, plutonium (plu), is located between the PCNA and RpS18 genes at position 56F on chromosome arm 2R. We have used a comparative genomic approach to investigate the evolution of the plu gene and to locate conserved cis-acting elements for plu, RpS18 and PCNA. Using primers within coding regions of PCNA and RpS18, we amplified and sequenced the intervening region from twelve Drosophila species. In each species, this region contains a plu gene resembling the D. melanogaster gene in size and in the number and position of introns. The predicted Plu sequence from the different species demonstrates that the first two ankyrin repeats are conserved. Of the transcriptional control elements of D. melanogaster PCNA, we found that three motifs 5' to the PCNA transcription unit are conserved in Drosophila species.
Renault
Andrew D
AD
Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, UK.
Axton
J Myles
JM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics*; D023061:Gene Order; D008969:Molecular Sequence Data; D010802:Phylogeny; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D012269:Ribosomal Proteins / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014157:Transcription Factors / Q000235:genetics*
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C085874:PLU protein, Drosophila; D018809:Proliferating Cell Nuclear Antigen; D012269:Ribosomal Proteins; D014157:Transcription Factors; C041184:ribosomal protein S18; D004247:DNA