Clear distinction of purine bases on the complementary strand by a fluorescence change of a novel fluorescent nucleoside.
A new fluorescent nucleoside, benzopyridopyrimidine (BPP), which can sharply distinguish between A and G bases opposite BPP has been devised. The base-pairing degeneracy of BPP strongly contributes to the sharp fluorescence change that is dependent on the type of purine bases opposite BPP. The hybridization of an ODN probe containing BPP with a target DNA facilitates the judgment with the naked eye of the type of purine base located at a specific site on the target DNA. The BPP-containing ODN is a very effective probe for A/G SNP typing.
Okamoto
Akimitsu
A
Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Kyoto 606-8501, Japan.
Tainaka
Kazuki
K
Saito
Isao
I
D004247:DNA / Q000032:analysis / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D009705:Nucleosides / Q000737:chemistry*; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide; D011687:Purines / Q000032:analysis*; D011743:Pyrimidines / Q000138:chemical synthesis / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D009705:Nucleosides; D009841:Oligonucleotides; D011687:Purines; D011743:Pyrimidines; C474393:benzopyridopyrimidine; D004247:DNA