A set of recombinant antigens from Echinococcus granulosus with potential for use in the immunodiagnosis of human cystic hydatid disease.
Several recombinant clones expressing antigens from Echinococcus granulosus were isolated previously from a parasite cDNA library using cystic hydatid disease (CHD) patients' sera or rabbit hyperimmune antiserum against a lipoproteic fraction from bovine cyst fluid. Six of these antigens were expressed in Escherichia coli and the purified recombinant proteins were tested in enzyme-linked immunosorbent assay (ELISA) for specific IgG with a panel of sera from patients with surgically confirmed (n = 58) or immunologically diagnosed (n = 71) CHD. Sera from clinically normal individuals (n = 203) and sera from individuals with other helminthic infections (n = 65) were assayed for the assessment of specificity. A cut-off value was determined by receiver-operating-characteristic plots for each antigen. A recombinant antigen B subunit (AgB8/2) presented the highest sensitivity (93.1%), considering the group of sera from patients with CHD surgically confirmed, and specificity (99.5%) and is proposed as the basis for an immunodiagnostic test. The other recombinant antigens tested presented sensitivities between 58.6% and 89.7%, and three of them were considered of complementary value. In subclass-specific ELISA, different IgG isotypes showed dominance in the response for each of the recombinant antigens. There was a clear predominance of IgG4 response for all antigens tested, indicating that this would be the subclass of choice to be assessed for these recombinant proteins.
Virginio
V G
VG
Laboratório de Biologia Molecular de Cestódeos, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Hernández
A
A
Rott
M B
MB
Monteiro
K M
KM
Zandonai
A F
AF
Nieto
A
A
Zaha
A
A
Ferreira
H B
HB
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology / Q000302:isolation & purification*; D019540:Area Under Curve; D016022:Case-Control Studies; D004443:Echinococcosis / Q000175:diagnosis / Q000276:immunology*; D004446:Echinococcus / Q000276:immunology*; D000939:Epitopes / Q000276:immunology / Q000302:isolation & purification; D004926:Escherichia coli / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D012372:ROC Curve; D011994:Recombinant Proteins / Q000276:immunology / Q000302:isolation & purification; D012698:Serologic Tests
D000947:Antigens, Helminth; D000939:Epitopes; D007074:Immunoglobulin G; D011994:Recombinant Proteins