[Use of mycolic acids analysis in diagnosis of tuberculosis and mycobacteriosis--three-year experience].
The aim of the study was to estimate the utility of the HPLC-based method of mycolic acids analysis to classify Mycobacterium species in routine diagnostic procedure on the basis of own three-year experience. 2142 patients' specimens were examined. 141 AFB were cultured. 36.2% strains were classified as M. tuberculosis complex by HPLC. The identification was confirmed by AMPLICOR MTB (Roche diagnostic, USA). M. xenopi (17.0%), M. kansasii (14.2%) and M. gordonae (14.2%) were the most frequent identified out of nontuberculous mycobacteria. Four mycobacteriosis cases were suspected because of repeated identification of the isolated strains. 136 strains on L-J slant shipped from other centres were identified. We confirm that the HPLC method is highly effective and specific for Mycobacterium species classification, which can be performed in no more than a couple of hours. In our opinion it is a very helpful tool, hard to replace in diagnostic procedure of tuberculosis and mycobacteriosis.
Walkiewicz
Renata
R
Klinika Chorób Wewnetrznych, Pneumonologii i Alergologii AM w Warszawie.
Safianowska
Aleksandra
A
Grubek-Jaworska
Hanna
H
Zalewska-Schönthaler
Natalia
N
Glapiński
Jarosław
J
Chazan
Ryszarda
R
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D009165:Mycobacterium Infections, Nontuberculous / Q000175:diagnosis* / Q000382:microbiology; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification / Q000378:metabolism; D009171:Mycolic Acids / Q000032:analysis*; D009170:Nontuberculous Mycobacteria / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism; D011044:Poland; D016133:Polymerase Chain Reaction / Q000379:methods; D012189:Retrospective Studies; D013045:Species Specificity; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology
D009171:Mycolic Acids
Zastosowanie analizy kwasów mikolowych w diagnostyce gruźlicy i mikobakterioz--3 lata doświadczeń.