title: Spatial and temporal pattern of Wnt-6 expression during chick development.
abstract: The WNT family of proteins is composed of several members. In the present study we isolated the full length chick Wnt-6 cDNA and analyzed its expression pattern by in situ hybridization during chick development. Wnt-6 expression is observed in the ectoderm from HH-stage 4 onwards. At HH-stages, 7-16 expression can be seen in the ectoderm overlying the segmental plate and the epithelial somite, while the ectoderm overlying the compartmentalized somite is Wnt-6 negative. Expression is also observed at the heart outflow tract and in the ectoderm overlying the pharyngeal arches. From HH-stages 17 to 27, expression is also observed at limb level, both in the dorsal and ventral ectoderm and a stronger expression in the dorsoventral boundary. Furthermore, expression in the ectoderm delimiting the somitic boundaries in the anteroposterior and mediolateral axis at limb level was observed, as well as in the ventral body wall. Expression becomes evident in the inner ear. From HH-stage 30 onwards, expression is restricted to the feather buds and to the gastrointestinal tract.
authors.affiliation: Institute of Anatomy II, University of Freiburg, Albertstrasse 17, 79104 Freiburg, Germany.
authors.forename: Marc
authors.initials: M
authors.lastname: Rodríguez-Niedenführ
authors.forename: Verena
authors.initials: V
authors.lastname: Dathe
authors.forename: Heinz Jürgen
authors.initials: HJ
authors.lastname: Jacob
authors.forename: Felicitas
authors.initials: F
authors.lastname: Pröls
authors.forename: Bodo
authors.initials: B
authors.lastname: Christ
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002642:Chick Embryo / Q000254:growth & development*; D004475:Ectoderm; D005241:Feathers / Q000196:embryology; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics; D019170:Somites
chemical_list: D011518:Proto-Oncogene Proteins