Kinetics of inhibition of ribonuclease P activity by peptidyltransferase inhibitors. Effect of antibiotics on RNase P.
A cell-free system derived from Dictyostelium discoideum has been used to study the kinetics of inhibition of RNase P by puromycin, amicetin and blasticidin S. Detailed kinetic analysis showed that the type of inhibition of RNase P activity by puromycin is simple competitive, whereas the type of inhibition by amicetin and blasticidin S is simple non-competitive. On the basis of Ki values amicetin is stronger inhibitor than puromycin and blasticidin S.
Kalavrizioti
Dimitra
D
Department of Biochemistry, School of Medicine, University of Patras, 26500 Patras, Greece.
Vourekas
Anastassios
A
Tekos
Apostolos
A
Tsagla
Antigoni
A
Stathopoulos
Constantinos
C
Drainas
Denis
D
D000818:Animals; D004023:Dictyostelium / Q000201:enzymology*; D004305:Dose-Response Relationship, Drug; D004722:Endoribonucleases / Q000037:antagonists & inhibitors*; D007700:Kinetics; D009705:Nucleosides / Q000378:metabolism / Q000493:pharmacokinetics; D010458:Peptidyl Transferases / Q000037:antagonists & inhibitors*; D011500:Protein Synthesis Inhibitors / Q000493:pharmacokinetics; D011691:Puromycin / Q000493:pharmacokinetics; D011741:Pyrimidine Nucleosides / Q000493:pharmacokinetics; D016337:RNA, Catalytic / Q000037:antagonists & inhibitors*; D043262:Ribonuclease P
D009705:Nucleosides; D011500:Protein Synthesis Inhibitors; D011741:Pyrimidine Nucleosides; D016337:RNA, Catalytic; C004413:amicetin; D011691:Puromycin; C004500:blasticidin S; D010458:Peptidyl Transferases; D004722:Endoribonucleases; D043262:Ribonuclease P