Methylation patterns and K-ras mutations in tumor and paired serum of resected non-small-cell lung cancer patients.
Gene methylation and K-ras mutations were examined in tumor and paired serum DNA of 50 resected non-small-cell lung cancer patients. RASSF1A, death associated protein kinase and target of methylation-induced silencing were methylated in 17/50 (34%), 23/50 (45%) and 18/50 (35%) tumors, respectively, and in 17/50 (34%), 20/50 (40%) and 17/50 (34%) sera, respectively. Methylation in tumor and serum were closely correlated (P=0.001), but no correlation was found with survival. Twelve K-ras mutations (cysteine) were found in serum and nine mutations were found in tumor (five cysteine, one alanine, one aspartic, one arginine, and one valine). K-ras mutations in serum correlated significantly with survival (P=0.01).
Ramirez
Jose Luis
JL
Medical Oncology Service, Hospital Germans Trias i Pujol, Ctra Canyet, s/n 08916, Barcelona, Spain.
Sarries
Carme
C
de Castro
Pedro Lopez
PL
Roig
Barbara
B
Queralt
Cristina
C
Escuin
Daniel
D
de Aguirre
Itziar
I
Sanchez
Jose Miguel
JM
Manzano
Jose Luis
JL
Margelí
Mireia
M
Sanchez
Jose Javier
JJ
Astudillo
Julio
J
Taron
Miquel
M
Rosell
Rafael
R
D000368:Aged; D000369:Aged, 80 and over; D051017:Apoptosis Regulatory Proteins; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000097:blood / Q000378:metabolism; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood* / Q000235:genetics*; D003062:Codon; D004247:DNA / Q000378:metabolism; D019175:DNA Methylation; D004252:DNA Mutational Analysis; D064550:Death-Associated Protein Kinases; D005260:Female; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood* / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009154:Mutation; D016133:Polymerase Chain Reaction; D011379:Prognosis; D013997:Time Factors
D051017:Apoptosis Regulatory Proteins; D003062:Codon; D004247:DNA; D064550:Death-Associated Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases