Surface alterations in the bovine pelvic peritoneum following rectal examination of reproductive organs: a scanning electron microscopy study.
The aim of the present study was to evaluate possible injury provoked by an intensive reproductive programme based on rectal palpation in the cow. Alterations to the mesothelial surface of the peritoneum and to the oviductal mucosa were explored by scanning electron microscopy (SEM). Nine multiparous Friesian cows were selected from each of two commercial dairy herds. Cows in herd 1 were maintained on a weekly reproductive health programme, while those in herd 2 were not subjected to gynaecological exploration. Surface features of the mesothelial cells of the reproductive tract and adjacent ligaments, and the oviductal mucosa were explored by SEM observation of tissue specimens from each animal. Six cows in the intensive reproductive programme showed microscopic alterations of the peritoneal surface. Strands were observed in the oviducts and adjacent ligaments in five of these cows. These strands, composed of filaments covered with mesothelial cells, were probably adhesions. Cells with abundant vacuoles on their surface were detected in the mesothelium corresponding to three animals, two of which also showed microscopic strands. No peritoneal surface alterations were observed in the cows from herd 2. No oviductal mucosal modifications were detected in either group. These findings suggest that intensive reproductive programmes based on rectal palpation may increase the risk of peritoneal surface alterations in the cow, but effects on reproductive physiology are unlikely.
Yániz
J
J
Departamento de Producción Animal, Escuela Politècnica Superior de Huesca, Universidad de Zaragoza, Spain. jyaniz@posta.unizar.es
Santolaria
P
P
López-Gatius
F
F
D000818:Animals; D002417:Cattle / Q000033:anatomy & histology* / Q000502:physiology; D005260:Female; D008855:Microscopy, Electron, Scanning / Q000662:veterinary; D010173:Palpation / Q000009:adverse effects / Q000662:veterinary*; D010537:Peritoneum / Q000033:anatomy & histology / Q000166:cytology / Q000648:ultrastructure*; D012098:Reproduction / Q000502:physiology