title: Expression of MUPP1 protein in mouse brain.
abstract: Localizing cell surface receptors to specific subcellular sites can be crucial for proper functioning. PDZ proteins apparently play central roles in such protein localizations. 5-HT(2C) receptors have previously been shown to interact with MUPP1, a multi PDZ domain protein, in heterologous systems and in rat choroid plexus. We now report the generation and characterization of two independent MUPP1 antisera, which recognise distinct areas of the mouse brain in agreement with previous in-situ hybridization studies. Our results indicate that MUPP1 immunoreactivity co-localizes with 5-HT(2A) or 5-HT(2C) receptor expression in all regions of the mouse brain, including the choroid plexus where 5-HT(2C) receptors are highly enriched.
authors.affiliation: Institute of Animal Physiology, Department of Biology, Ruhr-University Bochum, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Sitek
authors.forename: G
authors.initials: G
authors.lastname: Poschmann
authors.forename: K
authors.initials: K
authors.lastname: Schmidtke
authors.forename: C
authors.initials: C
authors.lastname: Ullmer
authors.forename: L
authors.initials: L
authors.lastname: Maskri
authors.forename: M
authors.initials: M
authors.lastname: Andriske
authors.forename: C C
authors.initials: CC
authors.lastname: Stichel
authors.forename: X-R
authors.initials: XR
authors.lastname: Zhu
authors.forename: H
authors.initials: H
authors.lastname: Luebbert
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry / Q000235:genetics; D002352:Carrier Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D008565:Membrane Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; C111442:MPDZ protein, human; D008565:Membrane Proteins; C491750:Mpdz protein, mouse; C491749:Mpdz protein, rat; D011994:Recombinant Proteins