Determination of hydroxyoctadecadienoic acids.
Oxidation of low-density lipoproteins (LDL) plays a crucial role in inflammatorydiseases and aging. The main oxidation products of LDL are stereoisomeric 9-hydroxy-10,12-octadecadienoic acids (9-HODEs) and 13-hydroxy-9,11-octadecadienoic acids (13-HODEs). Nevertheless the content of HODEs in natural oxidized LDL is low compared to other components, thus determination of HODEs requires a sample enrichment in most cases. Big losses are encountered during the necessary processing due to the instability of HODEs against acidic conditions. Therefore the use of labeled standards is required. Standards with an 18O label in the carboxylic group used previously may partly suffer a loss of the label by exchange with water. In this paper we describe an improved work-up procedure and the preparation of standards labeled with 18O in the hydroxylic group which is not exchangeable.
Youhnovski
Nikolay
N
Department of Organic Chemistry 1, Bayreuth University, Universitaetstr. 30, 95447 Bayreuth, Germany.
Schulz
Daria
D
Schwarz
Caroline
C
Spiteller
Gerhard
G
Schubert
Klaus
K
D000375:Aging / Q000502:physiology; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007202:Indicators and Reagents; D007249:Inflammation / Q000503:physiopathology; D008041:Linoleic Acids / Q000032:analysis* / Q000737:chemistry; D008077:Lipoproteins, LDL / Q000097:blood
D007202:Indicators and Reagents; D008041:Linoleic Acids; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein