[Influence of irradiation on the dynamic three-dimension distribution of abl and bcr genes in the interphase nuclei of IM-9 cell].
OBJECTIVE
To investigate the material foundation of the fusion of bcr and abl genes, and to explore the pathogenesis of chronic myeloid leukemia.


METHODS
By FISH combined with laser confocal scanning microscopy, the three-dimension (3D) distribution of bcr and abl genes in the interphase nuclei of normal and irradiated IM-9 cells was studied in each cell cycle phases.


RESULTS
abl and bcr genes distributed non-randomly in the interphase nuclei of IM-9 cells. abl gene preferably located at the outer layer and bcr near the core of the nucleus. The two genes were drawn near each other most in G(0) phase. The relative distance between the homologous genes was greater at proliferation phase than at quiescence phase. After irradiation, the relative distances from the two genes to the core and between the two genes were shortened, with the shortest distance between the two genes in S phase.


CONCLUSIONS
Irradiation could change the 3D-distribution of abl and bcr genes in the interphase nuclei of IM-9 cell and accelerate them to draw near each other.
Zhang
Qing
Q
Department of Hematology, Nanfang Hospital, The First Military Medical University, Guangzhou 510515, China.
Zhou
Shu-yun
SY
Liu
Xiao-li
XL
Niu
Chao
C
Xu
Lan
L
Chen
Sai-juan
SJ
D002467:Cell Nucleus / Q000235:genetics / Q000528:radiation effects* / Q000648:ultrastructure; D002478:Cells, Cultured; D005260:Female; D016044:Fusion Proteins, bcr-abl / Q000235:genetics / Q000528:radiation effects*; D050939:Gene Fusion / Q000528:radiation effects; D016313:Genes, abl / Q000235:genetics / Q000528:radiation effects*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase / Q000235:genetics / Q000528:radiation effects; D008214:Lymphocytes / Q000648:ultrastructure; D018613:Microscopy, Confocal; D051562:Proto-Oncogene Proteins c-bcr / Q000235:genetics / Q000528:radiation effects*
D016044:Fusion Proteins, bcr-abl; C496613:BCR protein, human; D051562:Proto-Oncogene Proteins c-bcr