title: Sequence of a cDNA encoding acetylcholinesterase from susceptible and resistant two-spotted spider mite, Tetranychus urticae.
abstract: Acetylcholinesterase (AChE) from two-spotted spider mites, Tetranychus urticae was compared between an organophosphate susceptible (TKD) and a resistant (NCN) strain. The AChE of TKD had lower affinity to acetylthiocholine and propionylthiocholine than that of NCN, and the inhibition of AChE by DDVP, ambenonium, eserine and n-methyl-eserine showed that NCN was more insensitive than TKD. AChE cDNA sequence was determined, and the 687 amino acids of primary structure were deduced. There were six replacements of amino acid residues in TKD and two in NCN. #F331(439)C was the only substitution unique to NCN, however, this mutation existed homozygously in only two out of nine mites. This residue is one of the gorge lining components, and #F331(439)C might act an important role in the sensitivity of AChE to the inhibitors.
authors.affiliation: Institute of Agriculture and Forestry, University of Tsukuba, Ibaraki, Tenodai Tsukuba 305-8572, Japan.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Anazawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Tomita
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Aiki
authors.forename: Toshinori
authors.initials: T
authors.lastname: Kozaki
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kono
mesh_terms: D000110:Acetylcholinesterase / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D004792:Enzyme Precursors / Q000737:chemistry / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics; D007305:Insecticide Resistance / Q000235:genetics; D007306:Insecticides; D008925:Mites / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D009943:Organophosphorus Compounds; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014101:Torpedo
chemical_list: D018076:DNA, Complementary; D004792:Enzyme Precursors; D007306:Insecticides; D009943:Organophosphorus Compounds; D000110:Acetylcholinesterase