title: Effect of zirconium(IV) complexes on the thermal and enzymatic stability of type I collagen.
abstract: Understanding the mechanism of stabilization of collagen is an important area of research. Metal ions are known to interact with collagen and bring about the stability of the same. In the present investigation, the interaction of zirconium(IV) complexes with collagen was studied. The effect of zirconium(IV) complexes, namely zirconium oxychloride and zirconium oxalate on the enzymatic and thermal stability of collagen was investigated. Zirconium has been found to increase the hydrothermal stability of the rat tail tendon (RTT) collagen fibers to about 8-10 degrees C more than that of the native collagen. The order of stabilization of zirconium(IV) complexes is zirconium oxychloride>zirconium oxalate. This could be due to the differences in the type of interaction with collagen, which is also reflected in the differences in the conformational changes of collagen brought about by the two complexes. Zirconium oxychloride, which forms tetrameric species in solution, has been shown to have better crosslinking with collagen as seen from viscometry studies and hence provides better enzymatic stability to collagen than zirconium oxalate, which largely forms monomeric species in solution.
authors.affiliation: Chemical Laboratory, Central Leather Research Institute, Adyar, Chennai, India.
authors.forename: Nishtar
authors.initials: N
authors.lastname: Nishad Fathima
authors.forename: Madhan
authors.initials: M
authors.lastname: Balaraman
authors.forename: Jonnalagadda
authors.initials: J
authors.lastname: Raghava Rao
authors.forename: Balachandran
authors.initials: B
authors.lastname: Unni Nair
mesh_terms: D000818:Animals; D002152:Calorimetry, Differential Scanning; D002942:Circular Dichroism; D024042:Collagen Type I / Q000737:chemistry / Q000378:metabolism*; D017364:Collagenases / Q000378:metabolism*; D008297:Male; D008958:Models, Molecular; D010070:Oxalates / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D014783:Viscosity; D015040:Zirconium / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D024042:Collagen Type I; D010070:Oxalates; D015040:Zirconium; D017364:Collagenases; C026090:zirconium oxychloride