title: [Sensitivity of microscopy for detection of mycobacterium tuberculosis and MOTT (mycobacteria other than tuberculosis) on the basis of analysis 22.218 clinical materials submitted in 1998-2001 to the Department of Microbiology in National Tuberculosis and Lung Diseases Research Institute in Warsaw, Poland].
abstract: The results of 22.218 respiratory specimens sent to our laboratory were studied to determine the sensitivity of the Ziehl-Neelsen (Z-N) stain and microscopy-fluorescence method for detection of M.tbc and MOTT. There were no AIDS patients among analyzed cases. Smears were positive for acid fast bacilli (AFB) 60.0% (480 of 800) of specimens growing M. tuberculosis and 25.1% (219 of 872) of specimens growing the six common species of MOTT. Smear positivity by species was 28.1% (141 of 502) for M. kansasii, 29.4% (43 of 146) for MAIC, 28.7% (35 of 122) for M. xenopi. No smear was positive for M. gordone (43 cultures), M. fortuitum (33 cultures), M. scrofulaceum (26 cultures). The rate of nonculturable mycobacteria on L-J medium was 0.2%; mean rate of contamination was 4.6%. We also analyzed the relation between the number of AFB seen on the smear and time of the growth of M. tuberculosis and relation between abundance of the culture growth and AFB seen on smears. These study suggest that the sensitivity of microscopy for Mycobacterium tuberculosis is comparable with the data of others authors. Sensitivity of microscopy is lower in MOTT detection than for M.tbc (p < 0.001). Time of growth and abundance of the M. tuberculosis cultures were adequate to AFB seen in microscopy.
authors.affiliation: Zakładu Mikrobiologii IGiChP, Warszawie.
authors.forename: Zofia
authors.initials: Z
authors.lastname: Zwolska
authors.forename: Ewa
authors.initials: E
authors.lastname: Augustynowicz-Kopeć
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Kostrzewa
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Swiderska
authors.forename: Magdalena
authors.initials: M
authors.lastname: Klatt
authors.forename: Albert
authors.initials: A
authors.lastname: Jaworski
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods; D003470:Culture Media / Q000592:standards; D006801:Humans; D008856:Microscopy, Fluorescence; D009165:Mycobacterium Infections, Nontuberculous / Q000175:diagnosis / Q000382:microbiology; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D009170:Nontuberculous Mycobacteria / Q000302:isolation & purification*; D011044:Poland; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D013183:Sputum / Q000382:microbiology; D013194:Staining and Labeling / Q000379:methods; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis / Q000382:microbiology
chemical_list: D003470:Culture Media
vernacular_title: Czułość metody bakterioskopowej w wykrywaniu pratków gruźlicy i MOTT na podstawie analizy 22.218 badań diagnostycznych przeprowadzonych w Zakładzie Mikrobiologii IGiCHP w okresie 1998-2001.