title: Removal of estrogenic activities of 17beta-estradiol and ethinylestradiol by ligninolytic enzymes from white rot fungi.
abstract: We investigated whether manganese peroxidase (MnP) and the laccase-mediator system with 1-hydroxybenzotriazole (HBT) as mediator can remove the estrogenic activities of the steroidal hormones 17beta-estradiol (E(2)) and ethinylestradiol (EE(2)). Using the yeast two-hybrid assay system, we confirmed that the estrogenic activities of E(2) and EE(2) are much higher than those of bisphenol A and nonylphenol. Greater than 80% of the estrogenic activities of E(2) and EE(2) were removed following 1-h treatment with MnP or the laccase-HBT system; extending the treatment time to 8h removed the remaining estrogenic activity of both steroidal hormones. HPLC analysis demonstrated that E(2) and EE(2) had disappeared almost completely in the reaction mixture after a 1-h treatment. These results strongly suggest that these ligninolytic enzymes are effective in removing the estrogenic activities of E(2) and EE(2).
authors.affiliation: Department of Forest Resources Science, Faculty of Agriculture, Shizuoka University, Ohya 836, Japan.
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Suzuki
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Hirai
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Murata
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Nishida
mesh_terms: D019149:Bioreactors; D002851:Chromatography, High Pressure Liquid; D004958:Estradiol / Q000302:isolation & purification / Q000378:metabolism*; D045166:Estradiol Congeners / Q000302:isolation & purification / Q000378:metabolism*; D004997:Ethinyl Estradiol / Q000302:isolation & purification / Q000378:metabolism*; D005658:Fungi; D042845:Laccase; D010088:Oxidoreductases / Q000494:pharmacology; D010544:Peroxidases / Q000494:pharmacology; D014874:Water Pollutants, Chemical / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D045166:Estradiol Congeners; D014874:Water Pollutants, Chemical; D004997:Ethinyl Estradiol; D004958:Estradiol; D010088:Oxidoreductases; D042845:Laccase; D010544:Peroxidases; C051129:manganese peroxidase