Trichomonas vaginalis: microtubule cytoskeleton distribution using fluorescent taxoid.
Trichomonas vaginalis is a flagellated parasitic protist of the human urogenital tract. The parasite has a poorly known cytoskeleton formed by an axostyle and a pelta, which are formed by stable structures such as microtubules, essential for the maintenance of cell shape and organization. FLUTAX-2 is an active fluorescent derivative of Taxol, binds to alphabeta-tubulin dimer polymerized. In this paper we present the analysis of microtubule distribution in living trophozoites of T. vaginalis using FLUTAX-2.
Lecke
Sheila B
SB
Laboratório de Parasitologia Clínica, Faculdade de Farmácia, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Tasca
Tiana
T
Souto
André A
AA
De Carli
Geraldo A
GA
D000818:Animals; D018385:Centrosome / Q000648:ultrastructure; D003599:Cytoskeleton / Q000648:ultrastructure*; D005407:Flagella / Q000648:ultrastructure; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000648:ultrastructure; D017239:Paclitaxel / Q000031:analogs & derivatives* / Q000737:chemistry; D043823:Taxoids; D014246:Trichomonas vaginalis / Q000648:ultrastructure*
D005456:Fluorescent Dyes; C413450:Flutax 2; D043823:Taxoids; D017239:Paclitaxel