Immunohistochemical identification of multipotent adult progenitor cells from human bone marrow after transplantation into the rat brain.
In the immunohistochemical analysis of the brain, tissue preparation and fixation are critical steps. During our studies of transplanting human bone marrow multipotent adult progenitor cells (hMAPCs) into the rat brain, we noticed that various methods of brain tissue preparation and fixation differentially influenced antigenic preservation in the transplants and in the host brain. Here, we report a simple, effective and reproducible method of tissue preparation and fixation that results in the immunohistochemical labeling of transplanted and host cells.
Zhao
Li-Ru
LR
Department of Neurosurgery, University of Minnesota Medical School, Lions Research Building, Room 421, 2001 Sixth Street S.E., Minneapolis 55455, USA.
Duan
Wei-Ming
WM
Reyes
Morayma
M
Verfaillie
Catherine M
CM
Low
Walter C
WC
D000818:Animals; D016477:Artifacts; D016026:Bone Marrow Transplantation; D002540:Cerebral Cortex / Q000166:cytology / Q000473:pathology / Q000601:surgery; D002544:Cerebral Infarction / Q000628:therapy*; D016616:Cryoultramicrotomy / Q000379:methods; D004210:Dissection / Q000379:methods; D005455:Fluorescent Antibody Technique / Q000379:methods; D005557:Formaldehyde; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods; D007150:Immunohistochemistry / Q000379:methods*; D020244:Infarction, Middle Cerebral Artery / Q000628:therapy*; D039902:Multipotent Stem Cells / Q000378:metabolism* / Q000637:transplantation*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D051381:Rats; D016707:Tissue Fixation / Q000379:methods*
D009419:Nerve Tissue Proteins; D005557:Formaldehyde