Chromosomal aberrations in Crepis capillaris cells detected by FISH.
Crepis capillaris (2n=6) is an excellent plant for the assay of chromosome aberrations after mutagenic treatment. It has simple karyotype: three pairs of morphologically distinct and relatively large chromosomes. The frequency of structural chromosome aberrations and micronuclei in root meristem cells has been used for evaluation of the genotoxicity of chemicals and environmental pollutants. The introduction of fluorescence in situ hybridization method allows more detailed detection and localization of chromosomal rearrangements not only in mitotic but also in interphase nuclei. We demonstrate a few examples of the detection of chromosomal aberrations using rDNA and telomeric sequences as probes for in situ hybridization to C. capillaris chromosomes.
Maluszynska
Jolanta
J
Department of Plant Anatomy and Cytology, University of Silesia, Katowice, Poland. maluszyn@us.edu.pl
Juchimiuk
Jolanta
J
Wolny
Elzbieta
E
D002869:Chromosome Aberrations / Q000139:chemically induced; D002875:Chromosomes / Q000187:drug effects / Q000235:genetics; D027468:Crepis / Q000187:drug effects / Q000235:genetics*; D004785:Environmental Pollutants / Q000032:analysis; D017404:In Situ Hybridization, Fluorescence / Q000379:methods* / Q000639:trends; D018519:Meristem / Q000187:drug effects / Q000235:genetics; D048629:Micronuclei, Chromosome-Defective / Q000235:genetics; D015162:Micronucleus Tests / Q000379:methods / Q000639:trends; D009152:Mutagenicity Tests / Q000379:methods* / Q000639:trends; D009153:Mutagens / Q000494:pharmacology*; D009154:Mutation / Q000187:drug effects / Q000235:genetics*
D004785:Environmental Pollutants; D009153:Mutagens