Gastric emptying in rats following administration of a range of different fats measured as acetaminophen concentration in plasma.
OBJECTIVE
To investigate the gastric emptying upon administration of ten different fats in order to determine whether major differences in fatty acid profiles resulted in differences in gastric emptying.


METHODS
Gastric emptying was measured as the appearance of acetaminophen in plasma which represents an indirect measure of gastric emptying. Emulsified fats with added acetaminophen were fed by gavage to rats, and the plasma concentration of acetaminophen was followed for 3 h by repeated blood sampling from the carotid artery. The fats administered included rapeseed, corn, and fish oils, lard, and cocoa butter as well as different structured lipids containing decanoic acid (10:0) and long-chain n-3 polyunsaturated fatty acids of marine origin. Overall, these fats had wide variations in fatty acid compositions and triacylglycerol structures.


RESULTS
No statistically significant differences were observed in gastric emptying between the groups fed the different fats, except for the emptying of tridecanoin (tri-10:0) that was statistically significantly slower than that of randomized oil, cocoa butter, and rapeseed oil (p < 0.05). The slower emptying of tri-10:0 could be caused by a lower caloric intake of this fat as compared with the other fats, because similar weights of fat were administered.


CONCLUSIONS
The gastric emptying of fat was not influenced by fatty acid composition and triacylglycerol structure of the fats administered.
Porsgaard
Trine
T
BioCentrum-DTU, Biochemistry and Nutrition, and LMC Center for Advanced Food Studies, Technical University of Denmark, Lyngby, Denmark. tpo@biocentrum.dtu.dk
Straarup
Ellen Marie
EM
Høy
Carl-Erik
CE
D000082:Acetaminophen / Q000008:administration & dosage / Q000097:blood*; D000284:Administration, Oral; D018712:Analgesics, Non-Narcotic / Q000008:administration & dosage / Q000097:blood*; D000704:Analysis of Variance; D000818:Animals; D004041:Dietary Fats / Q000008:administration & dosage* / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005746:Gastric Emptying / Q000187:drug effects / Q000502:physiology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D018712:Analgesics, Non-Narcotic; D004041:Dietary Fats; D005227:Fatty Acids; D000082:Acetaminophen