Cell-free synthesis of encephalomyocarditis virus.
We developed a system for complete replication of encephalomyocarditis virus (EMCV) in a test tube by using an in vitro translation extract from Krebs-2 cells. Efficient virus synthesis occurred in a narrow range of Mg(2+) and EMCV RNA concentrations. Excess input RNA impaired RNA replication and virus production but not translation. This suggests the existence of a negative-feedback mechanism for regulation of RNA replication by the viral plus-strand RNA or proteins.
Department of Biochemistry and McGill Cancer Center, McGill University, Montreal, Quebec, Canada H3G 1Y6. yuri.svitkine@mcgill.ca
Yuri V
YV
Svitkin
Nahum
N
Sonenberg
D000818:Animals; D002287:Carcinoma, Krebs 2 / Q000378:metabolism / Q000821:virology; D002474:Cell-Free System; D004686:Encephalomyocarditis virus / Q000235:genetics / Q000378:metabolism*; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis*; D014764:Viral Proteins / Q000378:metabolism; D014773:Virology / Q000379:methods; D014779:Virus Replication
D012367:RNA, Viral; D014764:Viral Proteins