[Human bone marrow mesenchymal stem cells express multiple hematopoietic growth factors].
To study the biological role of human cultured bone marrow mesenchymal stem cell (BM-MSC) in hematopoiesis by investigation of its expression of multiple hematopoietic growth factors, RT-PCR was used to analyze the expression of SCF, Flt3-ligand, TPO, LIF, G-CSF, GM-CSF, IL-3, IL-6 and IL-11 at mRNA level for human BM-MSC from healthy donors and patients with leukemia and lymphoma. BM-MSC were incubated with or without hydrocortison (HC). The results clearly showed that the cultured BM-MSC expressed mRNA of SCF, Flt3-ligand, TPO, LIF, IL-6 and IL-11 at passages 3 up to 15, but did not express G-CSF, GM-CSF and IL-3. The same expression pattern of above cytokines was seen also for the patient's BM-MSC. HC was able to induce BM-MSC to express G-CSF but not to express GM-CSF. BM-MSC seemed not to change morphologically after incubation with HC for up to 21 days. In conclusion, both normal and patient BM-MSC should be potential to promote hematopoiesis according to their expression of multiple hematopoietic cytokines, and HC is able to induce hematopoietic growth factor expression.
Department of Hematology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Guang-Rong
GR
Zhu
Xiao-Yu
XY
Zhou
Hua
H
Lu
Jian-Wei
JW
Zhou
Ai-Ping
AP
Li
Wei
W
Xu
Jian-Yong
JY
Li
Cheng-Ya
CY
Wang
D001854:Bone Marrow Cells / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D016179:Granulocyte Colony-Stimulating Factor / Q000235:genetics; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics; D016298:Hematopoietic Cell Growth Factors / Q000235:genetics*; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D016298:Hematopoietic Cell Growth Factors; D012333:RNA, Messenger; D016179:Granulocyte Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006854:Hydrocortisone