Cloning of the gene coding for Staphylococcus intermedius exfoliative toxin and its expression in Escherichia coli.
An exfoliative toxin (SIET)-producing strain (D-52) of Staphylococcus intermedius derived from canine pyoderma did not possess large plasmids. Therefore, the gene coding for SIET was considered to be located on the chromosomal DNA. The SIET gene was cloned from the chromosomal DNA of S. intermedius and was expressed in Escherichia coli. The nucleotide sequence of the SIET gene consists of a coding region of 990 bp specifying a polypeptide of 330 amino acid residues, which included a putative 42-residue signal sequence.
Department of Veterinary Microbiology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori 034-86280, Japan.
Rie
R
Terauchi
Hisaaki
H
Sato
You
Y
Endo
Chikara
C
Aizawa
Nobutoshi
N
Maehara
D000595:Amino Acid Sequence; D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D002645:Chickens; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005083:Exfoliatins / Q000096:biosynthesis / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013210:Staphylococcus / Q000235:genetics* / Q000378:metabolism
D001427:Bacterial Toxins; D004269:DNA, Bacterial; D005083:Exfoliatins; D011994:Recombinant Proteins