Sequence analysis of the N, P, M and F genes of Canadian human metapneumovirus strains.
The complete nucleotide sequences of the nucleoprotein (N), phosphoprotein (P), matrix protein (M), and fusion protein (F) genes of 15 Canadian human metapneumovirus (hMPV) isolates were determined. Phylogenetic analysis revealed two distinct genetic clusters, or groups for each gene with additional sequence variability within the individual groups. Comparison of the deduced amino acid sequences for the N, M and F genes of the different isolates revealed that all three genes were well conserved with 94.1-97.6% identity between the two distinct clusters The P gene showed more diversity with 81.6-85.7% amino acid identity for isolates between the two clusters, and 94.6-100% for isolates within the same cluster.
National Microbiology Laboratory, Canadian Science Center for Human and Animal Health, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3R2. nathalie_batien@hc-sc.gc.ca
Nathalie
N
Bastien
Susan
S
Normand
Tracy
T
Taylor
Diane
D
Ward
Teresa C T
TC
Peret
Guy
G
Boivin
Larry J
LJ
Anderson
Yan
Y
Li
D000595:Amino Acid Sequence; D001483:Base Sequence; D002170:Canada; D017931:DNA Primers; D006801:Humans; D029121:Metapneumovirus / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014763:Viral Matrix Proteins / Q000737:chemistry / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics*
D017931:DNA Primers; D014763:Viral Matrix Proteins; D014764:Viral Proteins