Effect of alpha-tocopherol on the oxidative modification of apolipoprotein E in human very-low-density lipoprotein.
The oxidative modification of apolipoprotein (apo) E and lipid peroxidation in human very-low-density lipoprotein (VLDL) induced by peroxynitrite and cupric ions in vitro were strongly suppressed by enrichment with alpha-tocopherol (alpha-Toc; 170 microM). Alpha-Toc also suppressed the decrease in the heparin-binding activity of apoE in the VLDL oxidation. These results suggest that alpha-Toc protected apoE in VLDL from oxidative stress.
Department of Biochemistry and Biomolecular Recognition, Yamaguchi University School of Medicine, Ube 755-8505, Japan.
Hirofumi
H
Arai
Koji
K
Uchida
Kenji
K
Fukunaga
Yuji
Y
Nagasaka
Satoshi
S
Mohri
Hiroko
H
Furumoto
Yasuhiro
Y
Kuramitsu
Kazuyuki
K
Nakamura
D001057:Apolipoproteins E / Q000378:metabolism*; D019327:Copper Sulfate / Q000494:pharmacology; D006493:Heparin / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008079:Lipoproteins, VLDL / Q000378:metabolism*; D008981:Molsidomine / Q000031:analogs & derivatives* / Q000494:pharmacology; D010084:Oxidation-Reduction; D024502:alpha-Tocopherol / Q000494:pharmacology*
D001057:Apolipoproteins E; D008079:Lipoproteins, VLDL; C002385:linsidomine; D006493:Heparin; D008981:Molsidomine; D024502:alpha-Tocopherol; D019327:Copper Sulfate