Improvement of mupirocin E-test for susceptibility testing of Staphylococcus aureus.
Interpretation of the mupirocin E-test for low-level mupirocin-resistant Staphylococcus aureus strains has been improved by adding the indicator dye tetrazolium. E-tests were compared with agar dilution methods for assessing mupirocin susceptibility. MICs obtained by the agar dilution method and E-tests showed 89.3% agreement within 2 log(2) dilution criteria. The agreement between MICs increased to 100% in the 1 log(2) dilution definition when the indicator dye tetrazolium was added to the E-test. The use of the E-test with tetrazolium reduction is more accurate for determining mupirocin MICs for S. aureus strains.
Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Brazil 2Departamento de Patologia, Universidade Federal Fluminense, Rio de Janeiro, RJ, Brazil.
Pedro Juan José
PJJ
Mondino
Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Brazil 2Departamento de Patologia, Universidade Federal Fluminense, Rio de Janeiro, RJ, Brazil.
Kátia Regina
KR
Netto Dos Santos
Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Brazil 2Departamento de Patologia, Universidade Federal Fluminense, Rio de Janeiro, RJ, Brazil.
Maria
M
do Carmo de Freire Bastos
Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Brazil 2Departamento de Patologia, Universidade Federal Fluminense, Rio de Janeiro, RJ, Brazil.
Marcia
M
Giambiagi-deMarval
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003428:Cross Infection / Q000382:microbiology; D024881:Drug Resistance, Bacterial; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry; D008826:Microbial Sensitivity Tests / Q000379:methods / Q000592:standards*; D016712:Mupirocin / Q000494:pharmacology*; D009580:Nitroblue Tetrazolium / Q000737:chemistry; D015588:Observer Variation; D015203:Reproducibility of Results; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects*
D000900:Anti-Bacterial Agents; D007202:Indicators and Reagents; D009580:Nitroblue Tetrazolium; D016712:Mupirocin