Apoptosis of endothelial cells may be mediated by genes of peroxisome proliferator-activated receptor gamma 1 (PPARgamma 1) and PPARalpha genes.
Apoptosis in human umbilical vein endothelial cells (HUVECs) was prevented by transfection with the gene for the human full-length peroxisome proliferator-activated receptor alpha (PPARalpha), or acyl-coenzyme A synthetase (AcylCS) into HUVECs. In contrast, ligands/activators of PPARgamma 1 induced apoptosis by a cytochrome c-dependent mechanism in HUVECs transfected with human full-length PPARgamma 1, but not in hepatocytes. Co-transfection of PPARgamma 1 and PPARalpha protected the HUVEC apoptosis. The results suggest that the apoptosis of endothelial cells may be mediated by genes of PPARgamma1 and PPARalpha.
Inoue
Ikuo
I
Fourth Department of Medicine, Saitama Medical School, 38 Morohongo, Moroyama, Iruma-gun, Saitama 350-0495, Japan. i1901018@saitama-med.ac.jp
Hayashi
Kenji
K
Yagasaki
Fumiharu
F
Nakamura
Koh-ichi
K
Matsunaga
Toshiyuki
T
Xu
Haiyuan
H
Inukai
Koh-ichi
K
Awata
Takuya
T
Komoda
Tsugikazu
T
Katayama
Shigehiro
S
D017209:Apoptosis / Q000235:genetics* / Q000502:physiology; D002478:Cells, Cultured; D003066:Coenzyme A Ligases / Q000235:genetics / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism; D006801:Humans; D050356:Lipid Metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D014471:Umbilical Veins
D029968:Escherichia coli Proteins; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors; D003066:Coenzyme A Ligases; C085934:acyl-coenzyme A synthetase, E coli