Fungi allergens produced by solid-state fermentation process: optimization and allergen characterization.
Allergenic extracts were produced from Drechslera (Helminthosporium) monoceras biomass cultured by solid-state fermentation using wheat bran as the substrate. The main fermentation variables were selected by statistical design, and the optimized biomass yield (1.43 mg/[g of dry substrate   d]) was obtained at pH 9.5 and 45.8% moisture. The allergenic extracts were produced from crude extract by protein precipitation and polyphenol removal. Proteins in the range of 16-160 kDa were identified in the extracts. Their reactions in patients were characterized by in vivo cutaneous tests (positive in 40% of the atopic patients) and by dot-blotting assays.
Hasan
Salah D M
SD
School of Chemical Engineering, State University of Campinas, PO Box 6066, 13083-970, Campinas-SP, Brazil.
Gambale
Walderez
W
Zollner
Ricardo L
RL
Santana
Maria H A
MH
D000485:Allergens / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D018533:Biomass; D016001:Confidence Intervals; D005285:Fermentation; D006375:Helminthosporium / Q000254:growth & development / Q000276:immunology* / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics
D000485:Allergens