[Effects of calcium channel antagonist on gastrin-induced proliferation of HT29 colon carcinoma cells].
OBJECTIVE
This study aimed to assess the effects of nifedipine on gastrin-induced proliferation of HT29 colon carcinoma cells and inquire into the possible mechanisms.


METHODS
Flow cytometry was used to monitor the cytoplasmic free calcium; MTT colorimetry was used to determine the proliferation of HT29 cells.


RESULTS
The results showed that 2.5 x 10(-6) mol/L pentagastrin (PG) induced a quick rise of intracellular free calcium ([Ca2+]i) (P < 0.01). 10(-5) mol/L nifedipine can significantly inhibited the rise of [Ca2+]i induced by pentagastrin (P < 0.01), in parallel, at growth assay we demonstrated that 10(-5)-10(-6) mol/L nifedipine could obviously block the increase in cell number elicited by 2.5 x 10(-6) mol/L PG.


CONCLUSIONS
These data indicate that nifedipine can stop the influx of Ca2+ and hence inhibit the pentagastrin-induced proliferation.
Department of Internal Medicine, First Affiliated Hospital, WCUMS, Chengdu 610041, China.
H
H
Wu
Z
Z
Zhang
X
X
Huang
Y
Y
Wei
S
S
Lei
D001693:Biological Transport, Active; D002118:Calcium / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D019073:HT29 Cells / Q000166:cytology*; D006801:Humans; D009543:Nifedipine / Q000494:pharmacology*; D010418:Pentagastrin / Q000037:antagonists & inhibitors*
D002121:Calcium Channel Blockers; D010418:Pentagastrin; D009543:Nifedipine; D002118:Calcium