Alkaline treatment has contrasting effects on the structure of deglycosylated and glycosylated forms of glucose oxidase.
X-ray crystallographic studies on glucose oxidase showed a strong interaction between carbohydrate and protein moieties of the glycoprotein. However, experimental studies under physiological conditions reported no influence of carbohydrate moiety on the structural and functional properties of glucose oxidase. In order to demonstrate the role of carbohydrate moiety on the structure and stability, we carried out a detailed comparative study on the pH-induced structural changes in the native and deglycosylated forms of glucose oxidase. Our studies demonstrate that at physiological pH both forms of enzyme have very similar structural and stability properties. Acid denaturation also showed similar structural changes in both forms of the enzyme. However, on alkaline treatment contrasting effects on the structure and stability of the two forms of enzyme were observed. The glycosylated enzyme undergoes partial unfolding with decreased stability at alkaline pH; however, a compaction of native conformation and enhanced stability of enzyme was observed for the deglycosylated enzyme under similar conditions. This is the first experimental demonstration of the influence of carbohydrate moiety on structure and stability of glucose oxidase. The studies also indicate the importance of pH studies in evaluating the effect of carbohydrate moiety on the structural and stability properties of glycoprotein.
Division of Molecular and Structural Biology, Central Drug Research Institute, Lucknow 226001, India.
Md Sohail
MS
Akhtar
Vinod
V
Bhakuni
D002241:Carbohydrates / Q000737:chemistry; D002850:Chromatography, Gel; D002942:Circular Dichroism; D003432:Cross-Linking Reagents / Q000494:pharmacology; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism*; D006031:Glycosylation; D006863:Hydrogen-Ion Concentration; D011487:Protein Conformation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D011506:Proteins / Q000378:metabolism; D013050:Spectrometry, Fluorescence
D002241:Carbohydrates; D003432:Cross-Linking Reagents; D011506:Proteins; D005949:Glucose Oxidase