Hypermutation in human B cells in vivo and in vitro.
We develop our previous observation that a subpopulation of circulating memory IgM(+)IgD(+)CD27(+)B cells belongs to a separate pathway of differentiation in humans. This subpopulation, which represents 5-25% of peripheral B cells, is also present in spleen in the same proportion and displays a marginal-zone-like B cell phenotype. In addition, we describe a short-time in vitro induction model for somatic hypermutation by using the BL2 Burkitt's lymphoma cell line stimulated by a combination of antibodies directed against different surface receptors. This short-time assay allows us to show that mutations are stably introduced in one DNA strand of the BL2 VH gene in the G1 phase of the cell cycle.
Weller
Sandra
S
INSERM U373, Faculté de Médecine Necker-Enfants Malades, Paris, France.
Faili
Ahmad
A
Aoufouchi
Said
S
Guéranger
Quetin
Q
Braun
Moritz
M
Reynaud
Claude-Agnès
CA
Weill
Jean-Claude
JC
D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D009154:Mutation; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000276:immunology
D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7