[Expression of a thermostable bacterial cellulase in transgenic tobacco plants].
The bacterial gene of the thermostable endo-beta-1,4-glucanase (cellulase) was shown to retain its activity and substrate specificity when expressed in transgenic tobacco plants. The leader peptide of the carrot extensin was efficient in transferring the bacterial enzyme into the apoplast. The expression of the bacterial cellulase gene leads to changes in the plant tissue morphology. In the transgenic plant lines, regeneration of primary shoots from callus occurred at the three to five times higher cytokinin (6-BAP) concentration than in control plants. The transgenic plants that expressed the bacterial gene exhibited increased business and altered leaf shape. The transgenic plants developed can be used as models for studying the cellulases role and function in plants.
Abdeev
R M
RM
Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 119991 Russia.
Goldenkova
I V
IV
Musiĭchuk
K A
KA
Piruzian
E S
ES
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002480:Cellulase / Q000235:genetics* / Q000378:metabolism; D018552:Daucus carota / Q000235:genetics; D004795:Enzyme Stability; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D005822:Genetic Vectors; D006023:Glycoproteins / Q000235:genetics; D010641:Phenotype; D018515:Plant Leaves / Q000033:anatomy & histology; D010940:Plant Proteins / Q000235:genetics; D018520:Plant Shoots / Q000187:drug effects / Q000502:physiology; D030821:Plants, Genetically Modified; D014026:Nicotiana / Q000235:genetics*; D014170:Transformation, Genetic
D001426:Bacterial Proteins; D006023:Glycoproteins; D010940:Plant Proteins; C005488:extensin protein, plant; D002480:Cellulase