Kallikrein-like prorenin-converting enzymes in inbred hypertensive mice.
Kallikreins are a group of serine proteases and are distinguished by having serine residue at their active site. Their general function is to convert inactive pro-peptide into its biologically active form. In recent years, emerging evidence indicates that some kallikrein-kinin enzymes also play a role in the modulation of renin-angiotensin system. These kallikrein-like prorenin converting enzymes act on renin-angiotensin by converting prorenin into biologically active renin. In this investigation, kallikrein-like prorenin converting enzyme (PRCE C) (mK9) is isolated from genetically inbred high blood pressure (BPH) and their normal counterparts (BPN) mice, and its protein levels are quantitated. Levels of mRNA expression are also compared. Additionally, localization of the enzyme is visualized by in situ hybridization histochemistry. Results indicated higher levels of PRCE C (mK9) enzyme in BPH mice in comparison to their normal counterparts. mRNA expression was also higher in BPH mice. In situ hybridization histochemistry results localized PRCE C (mK9) in the striated duct cells of submandibular gland.
Uddin
Mukarram
M
Department of Anatomy and Physiology, Meharry Medical College, 1005 D.B. Todd Boulevard, Nashville, TN 37208, USA. muddin@mmc.edu
Polley-Mandal
M
M
Beg
O U
OU
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D006973:Hypertension / Q000378:metabolism*; D017403:In Situ Hybridization; D007610:Kallikreins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D012333:RNA, Messenger / Q000378:metabolism; D013363:Submandibular Gland / Q000166:cytology / Q000378:metabolism
D012333:RNA, Messenger; D007610:Kallikreins; D003546:Cysteine Endopeptidases; C063281:prorenin processing enzyme