Combined histomorphometric and gene-expression profiling applied to toxicology.
We have developed a unique methodology for the combined analysis of histomorphometric and gene-expression profiles amenable to intensive data mining and multisample comparison for a comprehensive approach to toxicology. This hybrid technology, termed extensible morphometric relational gene-expression analysis (EMeRGE), is applied in a toxicological study of time-varied vehicle- and carbon-tetrachloride (CCl4)-treated rats, and demonstrates correlations between specific genes and tissue structures that can augment interpretation of biological observations and diagnosis.
Kriete
Andres
A
Tissue Informatics Inc, 711 Bingham Street, Suite 200, Pittsburgh, PA 15203, USA. akriete@tissueinformatics.com
Anderson
Mary K
MK
Love
Brad
B
Freund
John
J
Caffrey
James J
JJ
Young
M Brook
MB
Sendera
Timothy J
TJ
Magnuson
Scott R
SR
Braughler
J Mark
JM
D000818:Animals; D002251:Carbon Tetrachloride / Q000633:toxicity; D056486:Chemical and Drug Induced Liver Injury; D003627:Data Interpretation, Statistical; D020869:Gene Expression Profiling; D006651:Histocytochemistry / Q000379:methods; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008107:Liver Diseases / Q000235:genetics / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012313:RNA / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014116:Toxicology / Q000379:methods*
D012313:RNA; D002251:Carbon Tetrachloride