Effective chemical preservation of morphology of urinary erythrocytes.
Between 2 and 4 h after miction the morphology of urinary erythrocytes has changed, clouding the distinction between glomerular and non-glomerular bleeding in pediatric patients. Glomerular bleeding is characterized by microscopically visible alteration in urinary erythrocytes due to glomerular disease. Fixation by Cellfix, a formaldehyde-based fixative, allows the preservation of the morphology for at least 24 h and can be recommended for clinical practice. In our experience, thiomersal was not effective for preservation of the morphology of erythrocytes.
Department of Pediatric Nephrology, Institute of Pediatrics, RAMN, Moscow, Russia.
Olga
O
Komarova
Wim
W
van der Meer
Elena
E
Levtchenko
Leo
L
Monnens
D000050:Acanthocytes / Q000648:ultrastructure; D002648:Child; D004912:Erythrocytes / Q000648:ultrastructure*; D005404:Fixatives; D005557:Formaldehyde; D005921:Glomerulonephritis / Q000097:blood / Q000652:urine; D005922:Glomerulonephritis, IGA / Q000097:blood / Q000652:urine; D015432:Glomerulonephritis, Membranoproliferative / Q000097:blood / Q000652:urine; D006417:Hematuria / Q000097:blood*; D006801:Humans; D008858:Microscopy, Phase-Contrast; D013048:Specimen Handling / Q000379:methods*; D013997:Time Factors; D016707:Tissue Fixation / Q000379:methods*
D005404:Fixatives; D005557:Formaldehyde