Gold glyconanoparticles: synthetic polyvalent ligands mimicking glycocalyx-like surfaces as tools for glycobiological studies.
A simple and versatile methodology is described for tailoring sugar-functionalised gold nanoclusters (glyconanoparticles) that have 3D polyvalent carbohydrate display and globular shapes. This methodology allows the preparation of glyconanoparticles with biologically significant oligosaccharides as well as with differing carbohydrate density. Fluorescent glyconanoparticles have been also prepared for labelling cells in biological tests. The materials are water soluble, stable under physiological conditions and present an exceptional small core size. All of them have been characterised by (1)H NMR, UV and IR spectroscopy, TEM and elemental analysis. Their highly polyvalent network can mimic glycosphingolipid clustering and interactions at the plasma membrane, providing an controlled system for glycobiological studies. Furthermore, they are useful building blocks for the design of nanomaterials.
Grupo de Carbohidratos, Instituto de Investigaciones Químicas, Isla de la Cartuja, Américo Vespucio s/n, CSIC, 41092 Seville, Spain.
Africa G
AG
Barrientos
Jesús M
JM
de la Fuente
Teresa C
TC
Rojas
Asunción
A
Fernández
Soledad
S
Penadés
D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000737:chemistry*; D002855:Chromatography, Thin Layer; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D019276:Glycocalyx / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D007202:Indicators and Reagents; D008024:Ligands; D008863:Microspheres; D018716:Molecular Mimicry; D008969:Molecular Sequence Data; D013499:Surface Properties
D002241:Carbohydrates; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D008024:Ligands; D006046:Gold; D016650:Fluorescein-5-isothiocyanate