Characterization of cells prepared by dendritic cell-tumor cell fusion.
Dendritic cells (DCs) are professional antigen-presenting cells currently being discussed as a potent tool for antitumor vaccination strategies. The approach consisting of the in vitro generation of DC-tumor cell hybrids may be advantageous for individualized vaccines since there is no need for the determination of MHC-restricted tumor-associated antigens recognized by T cells. As recent vaccination studies gave varying results, we tested the impact of the fusion treatment on the cells used. Polyethylene glycol-induced fusion, as well as electrofusion, proved to be suitable for generating hybrid cells although at a low frequency. Of note, both methods also gave rise to DCs having phagocytosed apoptotic tumor cells. The expression of surface molecules relevant for specific T cell stimulation was not altered by the fusion procedure and the DCs were still functionally active as demonstrated by the secretion of IL-12 and the uptake of antigen. The cells were able to induce a tumor-specific T cell response in vitro and therefore deserve further investigation as potent tools for immunotherapy trials.
Dept. of Hematology and Oncology, University of Regensburg, Regensburg, Germany.
Eva
E
Gottfried
René
R
Krieg
Christian
C
Eichelberg
Reinhard
R
Andreesen
Andreas
A
Mackensen
Stefan W
SW
Krause
D015703:Antigens, CD / Q000032:analysis; D018122:B7-1 Antigen / Q000032:analysis; D051940:B7-2 Antigen; D002459:Cell Fusion; D002470:Cell Survival; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D007136:Immunoglobulins / Q000032:analysis; D018664:Interleukin-12 / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000032:analysis; D008562:Membrane Glycoproteins / Q000032:analysis; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D009369:Neoplasms / Q000276:immunology / Q000473:pathology; D010587:Phagocytosis / Q000276:immunology; D011092:Polyethylene Glycols / Q000494:pharmacology; D012235:Rhodamines; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology; D014407:Tumor Cells, Cultured / Q000276:immunology; D000097802:CD83 Antigen
D015703:Antigens, CD; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006684:HLA-DR Antigens; D007136:Immunoglobulins; D018664:Interleukin-12; D017493:Leukocyte Common Antigens; D008562:Membrane Glycoproteins; D011092:Polyethylene Glycols; D012235:Rhodamines; D000097802:CD83 Antigen; C116572:(((4-chloromethyl)benzoyl)amino)-tetramethylrhodamine; C497964:CD86 protein, human; C069306:5-chloromethylfluorescein