Microbiologic oxidation of isosafrole into piperonal.
The biotransformation of isosafrole by Cladosporium sphaerospermum yielded piperonal, which is a compound of great commercial importance in the flavor and fragrance industries. The experiments were performed in 500-mL conical flasks containing 100 mL of Czapek-modified medium in an orbital shaker with controlled agitation and temperature. Spores of C. sphaerospermum were used as inocula, and after 96 h of incubation the substrate was added to the culture. Samples of 2 mL were withdrawn at 24-h intervals and analyzed by gas chromatography, (GC) and/or GC/MS spectroscopy.
Santos
Alberdan Silva
AS
Instituto de Química, Universidade do Brasil, CT Bloco A Lab 641, Rio de Janeiro, RJ 21945-970, Brazil.
Pereira
Nei Pereira
NP
da Silva
Iracema I
II
Sarquis
Maria Inês
MI
Antunes
Octavio A C
OA
D001547:Benzaldehydes / Q000302:isolation & purification / Q000493:pharmacokinetics*; D052117:Benzodioxoles; D001711:Biotransformation; D002849:Chromatography, Gas / Q000379:methods; D002963:Cladosporium / Q000254:growth & development / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D007536:Isomerism; D010084:Oxidation-Reduction; D012451:Safrole / Q000302:isolation & purification / Q000493:pharmacokinetics*
D001547:Benzaldehydes; D052117:Benzodioxoles; C005454:piperonal; D012451:Safrole; C015959:isosafrole