Poly(ethyleneimine)-immobilized-cloth enzyme immunoassay for the detection of Salmonella lipopolysaccharide.
Poly(ethyleneimine) (PEI) was immobilized on non-woven polyester cloth and examined for application on a simple, rapid and economical "cloth enzyme immunoassay (CEIA)" which was developed originally as polymyxin-CEIA for the detection of Salmonella lipopolysaccharide (LPS). PEI-cloth regardless of the PEI molecular weight, but with the amine group contents of 0.1 to approximately 0.35 meq/g immobilized either in a physisorption-like or chemisorption-like manner, adsorbed LPS rapidly, preferentially and effectively. The captured LPS was then able to be detected qualitatively and quantitatively as an antigen by enzyme immunoassay. PEI-CEIA had a detection limit for Salmonella LPS of 10 ng/ml, which was equivalent to 1.6 x 10(5) cell/ml and was ten times more sensitive than polymyxin-CEIA. It was possible to detect Salmonella LPS in the presence of a 100-fold excess of E. coli LPS. PEI-CEIA was found to be more sensitive and much easier to carry out than polymyxin-CEIA but had the same advantages as polymyxin-CEIA.
Department of Applied Chemistry and Biochemistry, Faculty of Engineering, Kumamoto University, Kumamoto, Japan.
Kouchi
K
Matsumoto
Masayo
M
Sakata
Naoko
N
Iwahashi
Masashi
M
Kunitake
Masao
M
Miyazaki
Chuichi
C
Hirayama
D000327:Adsorption; D000818:Animals; D007124:Immunoenzyme Techniques / Q000379:methods*; D008070:Lipopolysaccharides / Q000032:analysis*; D011094:Polyethyleneimine / Q000737:chemistry / Q000493:pharmacokinetics; D011113:Polymyxins; D012486:Salmonella typhimurium / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
D008070:Lipopolysaccharides; D011113:Polymyxins; D011094:Polyethyleneimine