Hepatocyte ploidy in regenerating livers after partial hepatectomy, drug-induced necrosis, and cirrhosis.
The hepatocyte ploidy was investigated by flow cytometry in regenerating Sprague-Dawley rat livers following either drug-induced acute necrosis (single sublethal doses of D-galactosamine or thioacetamide) or drug-induced chronic cirrhosis (repeated thioacetamide injections for 10-18 weeks) and in regenerating livers following 70% partial hepatectomy and was compared with that of normal hepatocytes. Twenty-four hours after partial hepatectomy, a significant decrease in 2n (1 diploid nucleus) hepatocytes and a significant increase in 8n (1 octoploid nucleus) hepatocytes occurred. In contrast, 24 h following induction of acute hepatic failure by single D-galactosamine or thioacetamide injections, a significant increase in 2n hepatocytes was observed, whereas the proportion of 8n hepatocytes remained unchanged. The liver ploidy returned to basal values within 21 days in all cases. In cirrhotic livers induced by chronic thioacetamide injections, the rate of 2n hepatocytes was about ten times that of the controls having the same age, while 4n (1 tetraploid nucleus) and 8n hepatocytes were one third of controls. The binucleation rate was also significantly decreased.
Gandillet
A
A
Laboratoire de Chirurgie Expérimentale, Fondation Transplantation, Unité Inserm 544, Strasbourg, France.
Alexandre
Eliane
E
Royer
Cathy
C
Cinqualbre
J
J
Jaeck
D
D
Richert
Lysiane
L
D000818:Animals; D048429:Cell Size; D004171:Diploidy; D005434:Flow Cytometry; D005688:Galactosamine / Q000633:toxicity; D006498:Hepatectomy; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D008099:Liver / Q000187:drug effects; D008103:Liver Cirrhosis / Q000235:genetics* / Q000473:pathology; D008115:Liver Regeneration / Q000235:genetics*; D008297:Male; D008855:Microscopy, Electron, Scanning; D009336:Necrosis; D011003:Ploidies; D011123:Polyploidy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013853:Thioacetamide / Q000633:toxicity
D013853:Thioacetamide; D005688:Galactosamine