RNA polymerase holoenzyme: structure, function and biological implications.
The past three years have marked the breakthrough in our understanding of the structural and functional organization of RNA polymerase. The latest major advance was the high-resolution structures of bacterial RNA polymerase holoenzyme and the holoenzyme in complex with promoter DNA. Together with an array of genetic, biochemical and biophysical data accumulated to date, the structures provide a comprehensive view of dynamic interactions between the major components of transcription machinery during the early stages of the transcription cycle. They include the binding of sigma factor to the core enzyme, and the recognition of promoter sequences and DNA melting by holoenzyme, transcription initiation and promoter clearance.
Department of Microbiology and Immunology, SUNY Health Sciences Center, 450 Clarkson Avenue, Room BSB 3-27, Brooklyn, NY 11203, USA. serbor@aol.com
Sergei
S
Borukhov
Evgeny
E
Nudler
D001419:Bacteria / Q000201:enzymology*; D004259:DNA-Directed DNA Polymerase / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D020035:Holoenzymes / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D008957:Models, Genetic; D015394:Molecular Structure; D012808:Sigma Factor / Q000235:genetics / Q000378:metabolism; D019914:Taq Polymerase / Q000235:genetics; D014158:Transcription, Genetic
D020035:Holoenzymes; D012808:Sigma Factor; D019914:Taq Polymerase; C068508:Tth polymerase; D012321:DNA-Directed RNA Polymerases; D004259:DNA-Directed DNA Polymerase