Rapid detection of pulmonary tuberculosis using the BDProbeTEC ET Mycobacterium tuberculosis Complex Direct Detection Assay (DTB).
The ability to rapidly detect tubercle bacilli in respiratory secretions was determined for the BDProbeTEC ET Mycobacterium tuberculosis Complex Direct Detection Assay in comparison with the acid-fast smear (AFS). A total of 267 respiratory specimens obtained from 89 patients were evaluated. The DTB assay was positive in 70 of 78 culture positive specimens (89.7%) and 12 of 177 culture negative specimens (6.8%). The AFS was positive in 33 of 78 culture positive specimens (42.3%) and 3 of 186 culture negative specimens (1.6%). The sensitivity, specificity, positive predictive value, and negative predictive value of DTB assay were 89.7%, 93.7%, 85.4%, and 95.7%, respectively. The sensitivity of a single DBT (74.4%) was 2.1-times greater than three AFS (35.9%). The greater cost of the DTB assay compared to the AFS was compensated by its valuable information for the diagnosis and control of tuberculosis. These results demonstrated the clinical usefulness of the DTB assay for the rapid diagnosis of tuberculosis in respiratory specimens.
Huang
Tsi-Shu
TS
Section of Microbiology and Infectious Diseases, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.
Huang
Wen-Kuei
WK
Lee
Susan Shin-Jung
SS
Tu
Hui-Zin
HZ
Chang
Shu-Huei
SH
Liu
Yung-Ching
YC
D001431:Bacteriological Techniques / Q000191:economics / Q000379:methods*; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D003362:Cost-Benefit Analysis; D015342:DNA Probes; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques / Q000191:economics; D011933:Reagent Kits, Diagnostic / Q000191:economics; D012680:Sensitivity and Specificity; D013048:Specimen Handling; D013183:Sputum / Q000382:microbiology; D013624:Taiwan; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis*
D015342:DNA Probes; D011933:Reagent Kits, Diagnostic