The plasma superoxide scavenging activity in canine cancer and hepatic disease.
To clarify the relationship between plasma antioxidant activity and diseases in dogs, plasma samples were collected from 6 healthy dogs and 16 diseased dogs (6 dogs with cancer, 5 dogs with hepatic disease, and 5 dogs with inflammation ), and measured superoxide anion scavenging activities. Antioxidant activities of canine plasma were evaluated by measuring their superoxide anion (O(2)(-.)) scavenging activities with electron spin response spectroscopy combined with spin trapping reagent, 5,5-dimethyl-1-pyrroline-N-oxide (DMPO). Total O(2)(-.) scavenging activities in the presence of plasma of diseased dogs tended to be higher than those in healthy controls, especially significant higher activities in the presence of canine plasma of hepatic disease and inflammation were observed. In diseased dogs, KCN-insensitive activities, suggesting the activity of manganese-containing superoxide dismutase (Mn-SOD), were significantly higher than those in healthy controls. Therefore, it seems that there is a possibility of utilizing of plasma O(2)(-.) scavenging activity as one of clinical indicators for oxidative-related diseases such as cancer, hepatic disease and inflammation in dogs.
Department of Veterinary Internal Medicine, Faculty of Agriculture, Iwate University, Morioka, Japan.
Reeko
R
Sato
Osamu
O
Inanami
Bunei
B
Syuto
Jun
J
Sato
Mikinori
M
Kuwabara
Yoshihisa
Y
Naito
D000818:Animals; D016022:Case-Control Studies; D003497:Cyclic N-Oxides; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D004578:Electron Spin Resonance Spectroscopy / Q000662:veterinary; D005260:Female; D016166:Free Radical Scavengers / Q000097:blood*; D019271:Hypoxanthine / Q000378:metabolism; D007249:Inflammation / Q000378:metabolism / Q000662:veterinary*; D008107:Liver Diseases / Q000378:metabolism / Q000662:veterinary*; D008297:Male; D009369:Neoplasms / Q000378:metabolism / Q000662:veterinary*; D013113:Spin Labels; D013481:Superoxides / Q000378:metabolism*; D014969:Xanthine Oxidase / Q000378:metabolism
D003497:Cyclic N-Oxides; D016166:Free Radical Scavengers; D013113:Spin Labels; D013481:Superoxides; D019271:Hypoxanthine; C017245:5,5-dimethyl-1-pyrroline-1-oxide; D014969:Xanthine Oxidase