A microarray to analyze methylation patterns of p16(Ink4a) gene 5'-CpG islands.
OBJECTIVE
Aberrant DNA methylation of the CpG site is among the earliest and most frequent alterations in cancer. Several studies suggest that aberrant methylation on the CpG sites of the tumor suppressor gene is closely associated with carcinogenesis. However, large-scale analysis of candidate genes has so far been hampered by the lack of high throughput approach for analyzing methylation patterns. The aim of this study was to develop a new method to analyze methylation patterns of p16(Ink4a) gene.


METHODS
We selected a 336 bp segment of the 5' untranslated region and the first exon of the p16(Ink4a) gene, as the target sequence, which include the most densely packed CpG fragment of the islands containing 32 CpG sites. A set of oligonucleotide probes was designed to assemble a DNA microarray to discriminate the methylation patterns of several adjacent CpG sites.


RESULTS
Methylation patterns of human p16(Ink4a) gene were mapped and the results were validated by bisulphite DNA sequencing. A good reproducibility was observed in several parallel experiments.


CONCLUSIONS
The methylation oligonucleotide microarray can be applied as a useful and powerful tool to map methylation patterns in multiple CpG island sites.
Department of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
Peng
P
Hou
Meiju
M
Ji
Zhengchun
Z
Liu
Jiayao
J
Shen
L u
Lu
Cheng
Nongyue
N
He
Zuhong
Z
Lu
D001483:Base Sequence; D018899:CpG Islands / Q000235:genetics*; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics*; D019175:DNA Methylation; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism*; D013447:Sulfites
D019941:Cyclin-Dependent Kinase Inhibitor p16; D015345:Oligonucleotide Probes; D013447:Sulfites