Ras transgene expression in TG-AC mice treated with benzo[a]pyrene.
Transgene expression and skin tumorigenicity were investigated in transgenic TG-AC mice carrying the v-Ha-ras after treatment with benzo[a]pyrene (BP). Animals treated with 40 microg BP (x2/week/mouse) showed 100% tumor response after 25 weeks, as did 40% of the mice treated with 20 microg BP but 10 microg BP did not produce a tumor response. In the case of animals treated with 40 microg BP for 25 weeks, most of the tumors were proven to be carcinomas (80%, 4 out of 5 mice), and all tumors were shown to be positive in terms of transgene expression detected by in situ hybridization. These data suggest that BP was tumorigenic in a dose-dependent manner in TG-AC mice and that TG-AC mice were dependent on transgene expression during BP carcinogenesis.
Lee
Byung Mu
BM
Division of Toxicology, College of Pharmacy, SungKyunKwan University, Chunchun-dong, Suwon, Kyonggi-do, South Korea. bmlee@skku.ac.kr
Kim
Hyung Sik
HS
Kim
In Sun
IS
Ahn
Mi Young
MY
Chun
Sun Ah
SA
Yoo
Sun Dong
SD
Park
Yo An
YA
Choi
Sul Min
SM
Kim
Kyu Bong
KB
Han
Soon Young
SY
Park
Kui Lae
KL
Kwack
Seung Jun
SJ
Song
Hyun Sue
HS
Lee
Kyung Rim
KR
Bae
Hye Jin
HJ
Cho
Myung Haing
MH
D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D002273:Carcinogens / Q000633:toxicity*; D002277:Carcinoma / Q000139:chemically induced; D005260:Female; D017403:In Situ Hybridization; D051379:Mice; D008822:Mice, Transgenic; D012878:Skin Neoplasms / Q000139:chemically induced; D019076:Transgenes; D018631:ras Proteins / Q000096:biosynthesis* / Q000235:genetics
D002273:Carcinogens; D001564:Benzo(a)pyrene; D018631:ras Proteins