CelF of Orpinomyces PC-2 has an intron and encodes a cellulase (CelF) containing a carbohydrate-binding module.
A cDNA, designated celF, encoding a cellulase (CelF) was isolated from the anaerobic fungus Orpinomyces PC-2. The open reading frame contains regions coding for a signal peptide, a carbohydrate-binding module (CBM), a linker, and a catalytic domain. The catalytic domain was homologous to those of CelA and CelC of the same fungus and to that of the Neocallimastix patriciarum CELA, but CelF lacks a docking domain, characteristic for enzymes of cellulosomes. It was also homologous to the cellobiohydrolase IIs and endoglucanases of aerobic organisms. The gene has a 111-bp intron, located within the CBM-coding region. Some biochemical properties of the purified recombinant enzyme are described.
Center for Biological Resource Recovery, Department of Biochemistry and Molecular Biology, Life Science Building, The University of Georgia, Athens, GA 30602-7229.
Huizhong
H
Chen
Xin-Liang
XL
Li
David L
DL
Blum
Eduardo A
EA
Ximenes
Lars G
LG
Ljungdahl
D001483:Base Sequence; D001665:Binding Sites; D050260:Carbohydrate Metabolism; D020134:Catalytic Domain; D002480:Cellulase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D007438:Introns; D008969:Molecular Sequence Data; D020085:Neocallimastigales / Q000201:enzymology* / Q000235:genetics*; D016366:Open Reading Frames; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013379:Substrate Specificity
D017931:DNA Primers; D018076:DNA, Complementary; D011994:Recombinant Proteins; D002480:Cellulase