Immunomagnetic assay, classic culture method and fermentation tube test in the recovery of Escherichia coli O157 from sewage.
The diffusion of E. coli O157 was studied in the effluents of a large sewage treatment plant. Three methods were used: the Culture Method, Immunomagnetic Separation (IMS) and Fermentation Tube Test. Vero cells assay and PCR were used to assess toxin production and the presence of virulence genes. E. coli O157 were only detected using IMS and the Fermentation Tubes Test: one strain in raw sewage and 2 in a sample of disinfected effluent. The latter resulted positive for the production of verocytotoxin and possessed genes coding for vt2 and type gamma intimin. The best results were obtained with SMAC-CT-BCIG medium. The use of various different recovery techniques confirmed the difficulty involved in isolating E. coli O157 from water samples.
Department of Medicine and Public Health, University of Bologna, Italy.
F
F
Zanetti
G
G
De Luca
S
S
Morabito
R
R
Sacchetti
S
S
Stampi
D001431:Bacteriological Techniques; D003470:Culture Media; D004755:Enterobacteriaceae / Q000302:isolation & purification; D019453:Escherichia coli O157 / Q000235:genetics / Q000302:isolation & purification*; D005285:Fermentation; D018189:Immunomagnetic Separation / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D012722:Sewage / Q000382:microbiology*
D003470:Culture Media; D012722:Sewage