Maize (Zea mays)-derived bovine trypsin: characterization of the first large-scale, commercial protein product from transgenic plants.
Bovine trypsin (EC 3.4.21.4) is an enzyme that is widely used for commercial purposes to digest or process other proteins, including some therapeutic proteins. The biopharmaceutical industry is trying to eliminate animal-derived proteins from manufacturing processes due to the possible contamination of these products by human pathogens. Recombinant trypsin has been produced in a number of systems, including cell culture, bacteria and yeast. To date, these expression systems have not produced trypsin on a scale sufficient to fulfill the need of biopharmaceutical manufacturers where kilogram quantities are often required. The present paper describes commercial-level production of trypsin in transgenic maize (Zea mays) and its physical and functional characterization. This protease, the first enzyme to be produced on a large-scale using transgenic plant technology, is functionally equivalent to native bovine pancreatic trypsin. The availability of this reagent should allow for the replacement of animal-derived trypsin in the processing of pharmaceutical proteins.
Woodard
Susan L
SL
ProdiGene, Inc., 101 Gateway Blvd., Suite 100, College Station, TX 77845-4473, USA. swoodard@prodigene.com
Mayor
Jocelyne M
JM
Bailey
Michele R
MR
Barker
Donna K
DK
Love
Robert T
RT
Lane
Jeffrey R
JR
Delaney
Donna E
DE
McComas-Wagner
Janet M
JM
Mallubhotla
Hanuman D
HD
Hood
Elizabeth E
EE
Dangott
Lawrence J
LJ
Tichy
Shane E
SE
Howard
John A
JA
D000818:Animals; D002417:Cattle; D003001:Cloning, Molecular; D004789:Enzyme Activation; D005433:Flour; D006031:Glycosylation; D006801:Humans; D007700:Kinetics; D030821:Plants, Genetically Modified / Q000737:chemistry / Q000201:enzymology*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D012639:Seeds / Q000737:chemistry / Q000201:enzymology; D014357:Trypsin / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D014362:Trypsinogen / Q000378:metabolism; D003313:Zea mays / Q000737:chemistry / Q000201:enzymology / Q000235:genetics*
D011994:Recombinant Proteins; D014362:Trypsinogen; D014357:Trypsin