Inhibitory activity on lipid peroxidation of extracts from marine brown alga.
Several extracts from marine brown alga Sargassum micracanthum (Kuetzing) Endlicher were screened for their inhibitory activity on lipid peroxidation. In an in vitro study, methanol extract (Sm-M), chloroform/ methanol (3:1) extract and ethyl acetate fraction of Sm-M inhibited lipid peroxidation in rat liver homogenates. The IC(50) values were 0.70, 0.70 and 0.37 micro g/mL, respectively. These inhibitions were stronger than vitamin C and E. These extracts showed reductive activity on DPPH, the IC(50) values were 34, 37 and 11 micro g/mL, respectively. In an in vivo study, Sm-M had the effect on CCl4 induced liver injury in rats and Sm-M (120-1200 mg/kg, p.o.) lowered dose-dependently the level of lipid peroxidation in liver.
Mori
Jun
J
Lead Chemical Co., Ltd., Himata, Toyama, Japan. jun-mori@lead.chemical.co.jp
Matsunaga
Takayuki
T
Takahashi
Satoshi
S
Hasegawa
Chika
C
Saito
Haruo
H
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors; D002251:Carbon Tetrachloride / Q000008:administration & dosage / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D006834:Hydrazines / Q000037:antagonists & inhibitors; D020128:Inhibitory Concentration 50; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D000459:Phaeophyceae / Q000737:chemistry*; D010851:Picrates; D051381:Rats; D017208:Rats, Wistar; D014810:Vitamin E / Q000494:pharmacology
D000975:Antioxidants; D001713:Biphenyl Compounds; D006834:Hydrazines; D010851:Picrates; D014810:Vitamin E; D002251:Carbon Tetrachloride; C004931:1,1-diphenyl-2-picrylhydrazyl; D001205:Ascorbic Acid