Potassium and tetraphenylphosphonium ion-selective electrodes for monitoring changes in the permeability of bacterial outer and cytoplasmic membranes.
A tetraphenylphosphonium ion (TPP(+))-selective electrode, originally developed as a membrane potential indicator, is useful for measuring increases in the permeability of bacterial outer membranes induced by antimicrobial agents. The combination of this electrode with a potassium ion-selective electrode enabled us to determine changes in the permeability of bacterial outer and cytoplasmic membranes simultaneously. Outer membrane permeabilization induced by antimicrobial agents, chlorhexidine and polyhexamethylene biguanide (PHMB), as monitored with the TPP(+) electrode, correlated closely with the ability of the agents to release lipopolysaccharide (LPS) from the outer membrane.
Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama 700-8530, Japan.
Keiko
K
Yasuda
Chie
C
Ohmizo
Takashi
T
Katsu
D001645:Biguanides / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000187:drug effects* / Q000502:physiology*; D002710:Chlorhexidine / Q000494:pharmacology; D004202:Disinfectants / Q000494:pharmacology; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism; D017736:Ion-Selective Electrodes; D008070:Lipopolysaccharides / Q000378:metabolism; D009861:Onium Compounds / Q000378:metabolism*; D009943:Organophosphorus Compounds / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism*
D001645:Biguanides; D004202:Disinfectants; D008070:Lipopolysaccharides; D009861:Onium Compounds; D009943:Organophosphorus Compounds; C031233:polihexanide; D002710:Chlorhexidine; D011188:Potassium; C013289:tetraphenylphosphonium