Lens epithelial cells express CD95 and CD95 ligand treatment induces cell death and DNA fragmentation in vitro.
OBJECTIVE
Despite advances in intraocular lens design and material, posterior capsule opacification remains one of the major problems in modern cataract surgery. Therefore, the use of antiproliferative agents has been advocated. CD95 ligand (CD95L, Fas, Apo-1) is a death ligand that triggers apoptosis in susceptible target cells. Apoptosis allows for the safe disposal of cells without damaging the surrounding tissue. The goal of this study was to characterize and evaluate CD95L-induced cell death in cultured lens epithelial cells (LEC).


METHODS
Expression of CD95 in untreated porcine LEC was investigated by flow cytometry. Cell death after CD95L or CD95 agonistic antibody treatment was assessed by crystal violet assay and DNA fragmentation was measured by comet assay.


RESULTS
The presence of CD95 was observed in LEC. CD95L treatment resulted in a time--and concentration-dependent killing of LEC, which was synergistically enhanced by the addition of cyclohexamide. CD95L treatment induced DNA fragmentation.


CONCLUSIONS
The present study confirms the use of apoptosis-inducing CD95L in the inhibition of LEC proliferation. Further studies are needed before clinical application of CD95L to inhibit posterior capsule opacification will be feasible.
Hueber
A
A
Center of Ophthalmology, University of Cologne, Cologne, Germany. Arno.Hueber@Uni-Koeln.de
Eichholtz
C D
CD
Kociok
N
N
Esser
J M
JM
Esser
P J
PJ
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D020552:Comet Assay / Q000379:methods; D004247:DNA / Q000032:analysis; D053938:DNA Fragmentation; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D053222:Fas Ligand Protein; D005434:Flow Cytometry; D066298:In Vitro Techniques; D007908:Lens, Crystalline / Q000166:cytology*; D008024:Ligands; D008562:Membrane Glycoproteins / Q000494:pharmacology*; D013552:Swine; D019014:fas Receptor / Q000096:biosynthesis*
D053222:Fas Ligand Protein; D008024:Ligands; D008562:Membrane Glycoproteins; D019014:fas Receptor; D004247:DNA