Amplification of antioxidant activity and xanthine oxidase inhibition of catechin by enzymatic polymerization.
To amplify the antioxidant activity, we synthesized poly(catechin) by the enzyme-catalyzed oxidative coupling using horseradish peroxidase as a catalyst. The poly(catechin) showed great improvement in antioxidant activity such as radical scavenging activity against the superoxide anion and inhibition effects against free radical induced-oxidation of low-density lipoprotein, compared with a catechin monomer. In addition, poly(catechin) showed very high inhibition effects on xanthine oxidase activity, whereas the catechin monomer showed very less inhibition effects. The amplified activities might offer a high potential as a therapeutic agent for prevention of various free radicals and/or enzyme-related diseases.
Bio-oriented Technology Research Advancement Institute, Department of Materials Chemistry, Graduate School of Engineering, Kyoto University, Kyoto, 606-8501, Japan.
Motoichi
M
Kurisawa
Joo Eun
JE
Chung
Young Jin
YJ
Kim
Hiroshi
H
Uyama
Shiro
S
Kobayashi
D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism*; D002392:Catechin / Q000737:chemistry / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000378:metabolism; D011108:Polymers / Q000737:chemistry / Q000378:metabolism*; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism
D000975:Antioxidants; D004791:Enzyme Inhibitors; D011108:Polymers; D002392:Catechin; D014969:Xanthine Oxidase