Orientation of the 19S regulator relative to the 20S core proteasome: an immunoelectron microscopic study.
Specific labelling with monoclonal antibodies reveals that in regulator-proteasome complexes the asymmetric 19S regulator (PA700) binds to one or both terminal alpha-disks of the cylinder-shaped 20S core proteasome in such a way that its reclining front part is positioned in the vicinity of proteasome subunit alpha6. The protruding rear part of the regulator appears to be situated distal to the sites occupied by the subunits alpha2 and alpha3, respectively. When viewed from beta1/beta1' to beta4/beta4' along the polar 2-fold axis of the 20S proteasome core, the rear part of each 19S regulator cap appears to protrude clockwise. Thus, a defined alignment of the 19S regulator with respect to the single polar 2-fold rotational axis of the 20S core proteasome is obtained.
Klinische Biochemie und Pathobiochemie, Deutsches Diabetes-Forschungsinstitut, Leibniz-Institut an der Heinrich-Heine-Universität Düsseldorf, Auf'm Hennekamp 65, 40225, Düsseldorf, Germany. kopp@ddfi.uni-duesseldorf.de
Friedrich
F
Kopp
Lothar
L
Kuehn
D000911:Antibodies, Monoclonal; D003546:Cysteine Endopeptidases / Q000378:metabolism / Q000648:ultrastructure*; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D016253:Microscopy, Immunoelectron; D009097:Multienzyme Complexes / Q000378:metabolism / Q000648:ultrastructure*; D046988:Proteasome Endopeptidase Complex; D011487:Protein Conformation; D021122:Protein Subunits; D011506:Proteins / Q000378:metabolism / Q000648:ultrastructure*
D000911:Antibodies, Monoclonal; D009097:Multienzyme Complexes; C085834:PA700 proteasome activator; D021122:Protein Subunits; D011506:Proteins; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex