Fabrication and photoelectric response of poly(allylamine hydrochloride)/PM thin films by layer-by-layer deposition technique.
Thin films of poly(allylamine hydrochloride) (PAH) and bacteriorhodopsin (bR) embedded in purple membrane (PM) have been prepared by layer-by-layer (LBL) self-assembly technique. The results obtained by UV-Vis spectroscopy and atomic force microscopy (AFM) analysis showed that the biological activity of bR was preserved and PM fragments could be well oriented onto the ITO substrate. A photo-electrochemical cell with the structure of ITO/(PAH/PM)(n)/electrolyte (0.5M KCl)/Pt was fabricated and studied. The photocurrent peaks of (PAH/PM)(6) corresponding to light-on and light-off were about 200 and 100 nA/cm(2), respectively, with the former enhanced 30% higher than that of the reference films made of (PDAC/PM)(6).
Key Laboratory of Colloid and Interface Science, Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China.
Jinfang
J
Chu
Xingchang
X
Li
Jianping
J
Zhang
Ji'an
J
Tang
D001436:Bacteriorhodopsins / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry*; D004553:Electric Conductivity; D008027:Light; D018625:Microscopy, Atomic Force; D011073:Polyamines / Q000737:chemistry*; D018717:Purple Membrane / Q000737:chemistry* / Q000502:physiology / Q000648:ultrastructure; D013057:Spectrum Analysis
D001672:Biocompatible Materials; D011073:Polyamines; C063994:polyallylamine; D001436:Bacteriorhodopsins