Determination of thiobarbituric acid adduct of malondialdehyde using on-line microdialysis coupled with high-performance liquid chromatography.
An on-line analytical system for the continuous monitoring of malondialdehyde (MDA) was developed. This method involves the use of microdialysis perfusion, on-line derivatization and on-line HPLC analysis. This method gave a linear response for MDA concentrations and HPLC peak areas in the range from 0.051 microM to 2.43 microM. The intra-day (RSD = 1.6-10.5%) and inter-day (RSD = 1.1-9.3%) precisions were acceptable. The average in vitro probe recovery of MDA standard was 18.4 +/- 1.0%. The detection limit was 0.03 microM, corresponding to 0.6 pmol for an injection volume of 20 microl. This method was used for in vitro peroxidation investigations, which provided evidence for elevated MDA levels following the incubation of metal ions to a linoleic acid solution.
Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
Jenn-Yuan
JY
Sheu
Hsiao-Pei
HP
Ku
Wei-Chang
WC
Tseng
Min-Tzu
MT
Chen
Li-Yu
LY
Tsai
Yeou-Lih
YL
Huang
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008315:Malondialdehyde / Q000737:chemistry*; D017551:Microdialysis; D015203:Reproducibility of Results; D017392:Thiobarbituric Acid Reactive Substances / Q000737:chemistry*
D017392:Thiobarbituric Acid Reactive Substances; D008315:Malondialdehyde