A novel method of eliminating non-neuronal proliferating cells from cultures of mouse dorsal root ganglia.
1. We hypothesized that non-neuronal cells could be eliminated from primary dorsal root ganglion (DRG) cultures by including a DNA topoisomerase inhibitor (camptothecin) during culture. 2. Exposure to 20 microM camptothecin for 48 h, beginning at 3 days in vitro, reliably eliminates proliferating non-neuronal cells. 3. Following camptothecin treatment, neurons survived and continued to extend neurites for several weeks without obvious defects in morphology or viability. 4. Transient camptothecin exposure is therefore an efficient and fast-acting method to purify DRG neurons in culture.
Laboratory of Molecular Biology, College of Pharmacy and Nutrition, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Parker L
PL
Andersen
J Ronald
JR
Doucette
Adil J
AJ
Nazarali
D000818:Animals; D000831:Animals, Newborn; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D005727:Ganglia, Spinal / Q000166:cytology* / Q000502:physiology; D051379:Mice; D012583:Schwann Cells / Q000166:cytology / Q000502:physiology