Antioxidant activity of dietary oregano essential oil and alpha-tocopheryl acetate supplementation in long-term frozen stored turkey meat.
The effects of dietary oregano essential oil and alpha-tocopheryl acetate supplementation on the oxidative stability of long-term frozen stored turkey meat were investigated. Thirty 12-week-old turkeys, randomly divided into five groups, were given a basal diet or a basal diet supplemented with 200 mg of alpha-tocopheryl acetate kg(-1), or 100 or 200 mg of oregano oil kg(-1), or 100 mg of oregano oil plus 100 mg of alpha-tocopheryl acetate kg(-1) for 4 weeks prior to slaughter. Lipid oxidation in breast and thigh meat was assessed after 1, 3, 6, and 9 months of frozen storage at -20 degrees C prior to or following 7 days of refrigerated storage at 4 degrees C. Results showed that oregano oil increased the oxidative stability of breast and thigh meat during the frozen storage. Dietary oregano oil at the inclusion level of 200 mg kg(-1) feed was significantly (p < 0.05) more effective in delaying lipid oxidation compared to the level of 100 mg kg(-1), but equivalent to dietary alpha-tocopheryl acetate supplementation at 200 mg kg(-1), which in turn was inferior to dietary supplementation of 100 mg kg(-1) oregano essential oil plus 100 mg kg(-1) alpha-tocopheryl acetate that was significantly (p < 0.05) superior to all other treatments. Thigh meat was more susceptible to oxidation than breast meat, although the former contained alpha-tocopherol at markedly higher levels. Mean alpha-tocopherol levels in breast and thigh meat from all treatments decreased during the frozen storage, the decrease being sharper between 1 and 3 months of frozen storage for breast and between 3 and 6 months for thigh meat. Oregano oil supplementation increased (p < 0.05) the retention of alpha-tocopherol in meat, the increase being positively correlated with the supplementation level. However, the retention of alpha-tocopherol in meat could only partly elucidate the antioxidant activity exhibited by dietary oregano oil supplementation.
Laboratory of Animal Nutrition, School of Veterinary Medicine, Aristotle University, 54006 Thessaloniki, Greece. bots@vet.auth.gr
Nikolaos A
NA
Botsoglou
Alexandros
A
Govaris
Evropi N
EN
Botsoglou
Sophia H
SH
Grigoropoulou
George
G
Papageorgiou
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D019587:Dietary Supplements; D005260:Female; D005519:Food Preservation; D005615:Freezing; D015227:Lipid Peroxidation / Q000187:drug effects; D008460:Meat / Q000032:analysis; D009822:Oils, Volatile / Q000494:pharmacology*; D031341:Origanum / Q000737:chemistry*; D010938:Plant Oils / Q000494:pharmacology; D024505:Tocopherols; D014422:Turkeys; D024502:alpha-Tocopherol / Q000008:administration & dosage* / Q000031:analogs & derivatives* / Q000032:analysis
D000975:Antioxidants; D009822:Oils, Volatile; D010938:Plant Oils; D024502:alpha-Tocopherol; D024505:Tocopherols