Effect of blood withdrawal and angiotensin II on prolactin release in the tilapia, Oreochromis mossambicus.
Repeated blood withdrawal (5% of estimated blood volume at 0, 1, 4, 8, 24, 48 and 76 h) from tilapia acclimated to fresh water (FW) resulted in a marked increase in plasma levels of prolactin (PRL) during the first 8 h, reaching a peak above 300 ng/ml after 4 h. The increase in plasma PRL levels was significant except for the level after 72 h. A slight but significant decrease in plasma osmolality was observed at all time points after the blood withdrawal. Repeated blood withdrawal from fish acclimated to seawater (SW) resulted in a marked increase in plasma osmolality after 4 and 8 h. A significant increase was observed in plasma growth hormone (GH) in the fish in SW until the end of the experiment, but there was no change in plasma PRL. Plasma levels of cortisol were significantly higher in the fish in SW than in those in FW during the first 24 h. Blood withdrawal resulted in a significant reduction in hematocrit values in both FW- and SW-adapted fish, suggesting hemodilution. In a separate experiment, a single blood withdrawal (20% of total blood) stimulated drinking after 5 h, regardless of whether the fish were held in FW or SW. Plasma PRL level was also elevated following a single blood withdrawal in the fish acclimated to FW, but not in the fish in SW. Intraperitoneal injection of ANG II (1.0 microg/g) into the fish in FW significantly increased plasma PRL levels after 1 h. Activation of the renin-angiotensin system after blood withdrawal and the dipsogenic action of angiotensin II (ANG II) are well established in fish. The reduction in plasma osmolality after repeated blood withdrawal in FW and the increased osmolality in SW suggest that blood volume is restored, at least in part, by drinking environmental water. These results suggest that the marked increase in PRL concentration after blood withdrawal from the fish in FW is due, at least in part, to a facilitative effect between ANG II and reduced plasma osmolality.
Department of Animal Science and Hawaii Institute of Marine Biology, University of Hawaii, P.O. Box 1346, Coconut Island, Kaneohe, HI 96744, USA.
Thomas A
TA
Leedom
Tetsuya
T
Hirano
E Gordon
EG
Grau
D000064:Acclimatization / Q000502:physiology; D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D001800:Blood Specimen Collection; D001810:Blood Volume / Q000502:physiology / Q000662:veterinary*; D004327:Drinking Behavior / Q000187:drug effects / Q000502:physiology; D005618:Fresh Water; D013006:Growth Hormone / Q000097:blood / Q000378:metabolism; D006854:Hydrocortisone / Q000097:blood / Q000378:metabolism; D007274:Injections, Intraperitoneal; D009994:Osmolar Concentration; D011388:Prolactin / Q000097:blood / Q000378:metabolism*; D012623:Seawater; D017210:Tilapia / Q000097:blood / Q000502:physiology*
D000804:Angiotensin II; D011388:Prolactin; D013006:Growth Hormone; D006854:Hydrocortisone