[Effects of microwave irradiation on ATPase activity and voltage dependent ion channel of rat hippocampus cell membrane].
OBJECTIVE
To study the effect of microwave irradiation on hippocampus cell.


METHODS
Changes of ATPase activity and voltage dependent ion channel of hippocampus cell membrane were observed in mice exposed to 2 450 MHz microwave irradiation of 10 mW/cm2 from a physical therapy machine. Histochemical method and patch clamp method were used to determine the activity of Na+, K(+)-ATPase, Ca2+, Mg(2+)-ATPase and voltage dependent Na+, K+, Ca2+ channels respectively.


RESULTS
1) Na+, K(+)-ATPase activity of microwave irradiated mice showed no significant change as compared with the control, but the activity of Ca2+, Mg(2+)-ATPase decreased significantly (P< 0.05); 2) In microwave irradiated mice, Na+, K+, Ca2+, current inducement rate in hippocampus neuron decreased significantly, the membrane voltage of Na+ current peak shifted to depolarization, and the attenuation rate of Na+ current and current A inducement rate decreased significantly as compared with control mice.


CONCLUSIONS
Irradiation of 2 450 MHz microwave at a doze of 10 mW/cm2 was not fatal to mice hippocampus cell. But Ca2+, Mg(2+)-ATPase activity of hippocampal cell membrane and voltage dependent Na+, K+, Ca2+ ion channel of hippocampal nervous were affected which would affect study and memory.
Institute of Space Medico-Engineering, Beijing, China.
Ya-li
YL
Zhao
Hong-bo
HB
Ma
Jin-ping
JP
Song
Yu-hua
YH
Yang
Jing-sui
JS
Pu
Jian-quan
JQ
Zheng
D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism* / Q000528:radiation effects; D015220:Calcium Channels / Q000378:metabolism / Q000528:radiation effects; D002462:Cell Membrane / Q000201:enzymology / Q000378:metabolism / Q000528:radiation effects; D006624:Hippocampus / Q000166:cytology / Q000201:enzymology / Q000378:metabolism / Q000528:radiation effects*; D007473:Ion Channels / Q000378:metabolism* / Q000528:radiation effects; D051379:Mice; D008872:Microwaves; D015221:Potassium Channels / Q000378:metabolism / Q000528:radiation effects; D015222:Sodium Channels / Q000378:metabolism / Q000528:radiation effects; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism* / Q000528:radiation effects
D015220:Calcium Channels; D007473:Ion Channels; D015221:Potassium Channels; D015222:Sodium Channels; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase