[Synthesis and function analysis of a new thrombopoietin (TPO) mimic peptide].
In order to find a new TPO-mimic peptide with similar activity to TPO while reducing the side effects, a TPO-mimic peptide (P1) screened from a random peptide library was restructured. The new structure of the TPO mimic peptide (P2) was synthesized. After coupling P2 with Dextran 10 and performing intermolecular oxidation, dextran-coupled and dimerized form of P2 were obtained, naming D-P2 and (P2)(2) respectively. The activities of the peptides in vitro were measured with MTT method. The results showed that the EC50 of P2 was 20 nmol/L, 700 times higher than P1. The EC50 of D-P2 and (P2)(2) were 0.35 nmol/L and 0.14 nmol/L, respectively. After administrating to the mouse, the peptides increased the number of platelets in the blood circulation obviously without influence on other blood cells. In conclusion, the TPO-mimic peptides have prospects in treating diseases related with thrombocytopenia.
Li
Chao
C
Beijing Institute of Biotechnology, Beijing 100071, China. lichao@nic.bmi.ac.cn
Cheng
Du-Sheng
DS
Zhou
Yan-Rong
YR
Chen
Tian-Mi
TM
Huang
Pei-Tang
PT
D000818:Animals; D005260:Female; D008297:Male; D051379:Mice; D010976:Platelet Count; D013926:Thrombopoietin / Q000031:analogs & derivatives / Q000138:chemical synthesis* / Q000494:pharmacology
D013926:Thrombopoietin