Quantitative determination of free intracellular alpha-keto acids in neutrophils.
For the first time, a procedure is described for the quantitative analysis of free alpha-keto acid content in human neutrophils (PMNs) relative to single cell number by reversed-phase fluorescence high-performance liquid chromatography. The procedure is minimally invasive and is unsurpassed in the quality of PMN separation, ease of sample preparation as well as sample stability. This method can satisfy the rigorous demands for an ultra-sensitive, comprehensive and rapid intracellular alpha-keto acid analysis in particularly for the surveillance of severe diseases as well as cellular or organ dysfunction.
Mühling
Jörg
J
Department of Anaesthesiology, Intensive Medicine and Pain Therapy Justus Liebig University, Rudolf-Buchheim-Strasse 7, 35390, Giessen, Germany.
Fuchs
Markus
M
Campos
Marie E
ME
Gonter
Jens
J
Engel
Jörg M
JM
Sablotzki
Armin
A
Menges
Thilo
T
Weiss
Stefan
S
Dehne
Marius G
MG
Krüll
Matthias
M
Hempelmann
Gunter
G
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007651:Keto Acids / Q000097:blood*; D009504:Neutrophils / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods
D007651:Keto Acids