Dealing with different methods for Kluyveromyces lactis beta-galactosidase purification.
Several micro-scale chromatography-based procedures for purification of the beta-galactosidase from the yeast Kluyveromyces lactis were assayed. Purified enzyme was suitable to be used as antigen to induce polyclonal antibodies production. Specific staining of non-denaturing PAGE gels with chromogenic substrates allowed the determination of the number of subunits forming the native enzyme.
Becerra
M
M
Departamento de Bioloxía Celular e Molecular. Facultade de Ciencias. Campus da Zapateira s/n. 15071-A Coruña. Spain. migs@udc.es
Cerdãn
E
E
Gonzãlez Siso
M I
MI