Combined analysis of data from two granddaughter designs: A simple strategy for QTL confirmation and increasing experimental power in dairy cattle.
A joint analysis of five paternal half-sib Holstein families that were part of two different granddaughter designs (ADR- or Inra-design) was carried out for five milk production traits and somatic cell score in order to conduct a QTL confirmation study and to increase the experimental power. Data were exchanged in a coded and standardised form. The combined data set (JOINT-design) consisted of on average 231 sires per grandsire. Genetic maps were calculated for 133 markers distributed over nine chromosomes. QTL analyses were performed separately for each design and each trait. The results revealed QTL for milk production on chromosome 14, for milk yield on chromosome 5, and for fat content on chromosome 19 in both the ADR- and the Inra-design (confirmed within this study). Some QTL could only be mapped in either the ADR- or in the Inra-design (not confirmed within this study). Additional QTL previously undetected in the single designs were mapped in the JOINT-design for fat yield (chromosome 19 and 26), protein yield (chromosome 26), protein content (chromosome 5), and somatic cell score (chromosome 2 and 19) with genomewide significance. This study demonstrated the potential benefits of a combined analysis of data from different granddaughter designs.
Institut für Tierzucht und Tierhaltung, Christian-Albrechts-Universität, 24098 Kiel, Germany. jbennewitz@tierzucht.uni-kiel.de
Jörn
J
Bennewitz
Norbert
N
Reinsch
Cécile
C
Grohs
Hubert
H
Levéziel
Alain
A
Malafosse
Hauke
H
Thomsen
Ningying
N
Xu
Christian
C
Looft
Christa
C
Kühn
Gudrun A
GA
Brockmann
Manfred
M
Schwerin
Christina
C
Weimann
Stefan
S
Hiendleder
Georg
G
Erhardt
Ivica
I
Medjugorac
Ingolf
I
Russ
Martin
M
Förster
Bertram
B
Brenig
Fritz
F
Reinhardt
Reinhard
R
Reents
Gottfried
G
Averdunk
Jürgen
J
Blümel
Didier
D
Boichard
Ernst
E
Kalm
D000818:Animals; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping; D003612:Dairying; D003627:Data Interpretation, Statistical; D005260:Female; D005819:Genetic Markers / Q000235:genetics; D008957:Models, Genetic; D010375:Pedigree; D040641:Quantitative Trait Loci / Q000235:genetics*
D005819:Genetic Markers