The synergistic effect of conventional and sustained delivery of antioxidants on LNCaP prostate cancer cell line.
Studies have been limited involving antioxidants as a mode of treatment for prostate cancer. The goal of this study was to develop alternative treatment and prevention for the invasive behavior of prostatic mutations by exploring the synergistic effects of several commercially available antioxidants. The specific aims were to examine the viability, proliferation, and morphology of human prostate cells in culture upon treatment of low and high doses of vitamin E, selenium, and lycopene alone or in combination. In phase I, groups were treated with physiological doses of the experimental agents. In phase II, the same groups were treated in combination to observe any synergistic effect. Upon treatment with selenium, lycopene, and vitamin E, vitamin E treated groups demonstrated the greatest effect on most assays in the study. Data obtained from this study did not demonstrate that the antioxidants would eradicate LNCaP prostatic carcinoma cells; however, the suppression of the metabolic pathways, cellular damage, and lowered prostatic specific antigen was observed.
Clinical Health Sciences Graduate Program, School of Health Related Professions, Department of Pathology and Orthopedic Surgery and Rehab University of Mississippi Medical Center, Jackson, Mississippi, USA.
La'Toya Ross
LR
Richards
Hamed
H
Benghuzzi
Michelle
M
Tucci
James
J
Hughes
D000971:Antineoplastic Combined Chemotherapy Protocols / Q000008:administration & dosage*; D000975:Antioxidants / Q000008:administration & dosage*; D017209:Apoptosis / Q000187:drug effects; D002338:Carotenoids / Q000008:administration & dosage; D002452:Cell Count; D004305:Dose-Response Relationship, Drug; D006801:Humans; D000077276:Lycopene; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009363:Neoplasm Proteins / Q000378:metabolism*; D017430:Prostate-Specific Antigen / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D015203:Reproducibility of Results; D012643:Selenium / Q000008:administration & dosage; D012680:Sensitivity and Specificity; D014407:Tumor Cells, Cultured; D014810:Vitamin E / Q000008:administration & dosage
D000975:Antioxidants; D009363:Neoplasm Proteins; D014810:Vitamin E; D002338:Carotenoids; D008315:Malondialdehyde; D017430:Prostate-Specific Antigen; D012643:Selenium; D000077276:Lycopene