Identification of odor-active 3-mercapto-3-methylbutyl acetate in volatile fraction of roasted coffee brew isolated by steam distillation under reduced pressure.
In a roasted Arabica coffee brew, the potent roasty odor quality compound was identified as 3-mercapto-3-methylbutyl acetate by comparison of its Kovats gas chromatography retention index, mass spectrum, and odor quality to those of the synthetic authentic compound. 3-Mercapto-3-methylbutyl acetate has been identified for the first time in the coffee, and according to the results of the aroma extract dilution analysis, the contribution of this compound to the flavor of the roasted coffee brew varied depending on the degree of the coffee bean roasting. The concentration of this compound in the coffee brews as with 3-mercapto-3-methylbutyl formate increased with an increase in the degree of roasting. However, the slope of the amount of both esters was different, and 3-mercapto-3-methylbutyl acetate hardly increased with a low degree of roasting at more than a 21 luminosity (L)-value, but it rapidly increased when the roasting degree of the coffee beans reached the L-value of 18. These results suggested that the contribution of 3-mercapto-3-methylbutyl acetate to the overall flavor is peculiar to the flavor of the highly roasted coffee.
Material Research and Development Laboratories, Ogawa and Company, Ltd., 15-7 Chidori Urayasushi, Chiba 279-0032, Japan. kumazawa.kenji@ogawa.net
Kenji
K
Kumazawa
Hideki
H
Masuda
D000085:Acetates / Q000032:analysis*; D002849:Chromatography, Gas; D040503:Coffea / Q000737:chemistry; D003069:Coffee / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D006358:Hot Temperature; D006801:Humans; D009812:Odorants / Q000032:analysis*; D011312:Pressure; D012639:Seeds / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000032:analysis*; D014835:Volatilization
D000085:Acetates; D003069:Coffee; D013438:Sulfhydryl Compounds; C474495:3-mercapto-3-methylbutyl acetate