Reliability of phototoxic tests of fluoroquinolones in vitro.
OBJECTIVE
To make sure the reliability of phototoxic tests in vitro by comparing the phototoxic potential of 4 fluoroquinolones (FQ).


METHODS
Lomefloxacin (LFLX), sparfloxacin (SPFX), ciprofloxacin (CPFX), and norfloxacin (NFLX) were tested by Wistar rat phototoxic test and Balb/c mouse phototoxic test in vivo, and Chinese hamster V79 cell micronucleus test and NIH 3T3 MTT test in vitro under the different condition of UVA irradiation.


RESULTS
In all experiments, LFLX and SPFX showed higher phototoxic potential compared with the control (P<0.01 vs 1 % CMC), CPFX was mild (P<0.05 vs physiological saline), NFLX did not show phototoxicity in vivo, however at a higher concentration (10 micromol/L) in vitro, it also induced phototoxicity as other FQ.


CONCLUSIONS
There are good correlations between phototoxic tests in vivo and in vitro. These results ensure the validation of phototoxic tests in vitro.
State Key Laboratory of Drug Research, Shanghai Institue of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Ting
T
Zhang
Jun-Ling
JL
Li
Xiao-Chao
XC
Ma
Jian
J
Xin
Zeng-Hong
ZH
Tu
D016475:3T3 Cells; D000818:Animals; D000890:Anti-Infective Agents / Q000009:adverse effects*; D002939:Ciprofloxacin / Q000009:adverse effects; D006224:Cricetinae; D003412:Cricetulus; D017484:Dermatitis, Phototoxic / Q000209:etiology*; D005260:Female; D024841:Fluoroquinolones / Q000009:adverse effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D015162:Micronucleus Tests; D009643:Norfloxacin / Q000009:adverse effects; D015363:Quinolones / Q000009:adverse effects*; D051381:Rats; D017208:Rats, Wistar; D014466:Ultraviolet Rays
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D015363:Quinolones; D002939:Ciprofloxacin; C053091:lomefloxacin; D009643:Norfloxacin; C061363:sparfloxacin