[A analysis of ELISA on the time-related expression of IL-2 and TNF-alpha during the healing process of wound in rat skin].
OBJECTIVE
In order to explore the relationship between the expression change of cytokines and the wound age during the healing process of rats skin wound.


METHODS
A assay of ELISA were performed on intravital skin wounds(after incision 0.5-168 h) to detect the dynamics expression level of IL-2, TNF-alpha.


RESULTS
The level of IL-2 and TNF-alpha increased at 0.5 h after wounding, then got to a peak at 3 h and 1 h after injure respectively. Rebound of TNF and IL-2 levels were shown at 48 h after wounding, and both cytokine levels were inclined to elevating between 72 h and 168 h after wounding.


CONCLUSIONS
The cytokine level changes suggest they have a time-related expression during wounds healing process.
Wang
Hui-jun
HJ
Department of Pathology, First Military Medical University, Guangzhou 510515.
Ding
Yun-chuan
YC
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007376:Interleukin-2 / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000293:injuries* / Q000378:metabolism; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D014945:Wound Healing / Q000502:physiology*
D007376:Interleukin-2; D014409:Tumor Necrosis Factor-alpha