Lipase-catalysed transesterification of soya bean oil for biodiesel production during continuous batch operation.
The effects of temperature, oil/alcohol molar ratio and by-product glycerol were studied during Lipozyme TL IM-catalysed continuous batch operation when short-chain alcohols were used as the acyl acceptor. In non-continuous batch operation, the optimal oil/alcohol ratio and temperature were 1:4 and 40-50 degrees C; however, during the continuous batch operation, the optimal oil/alcohol ratio and temperature were 1:1 and 30 degrees C; 95% of enzymic activity remained after 10 batches when isopropanol was adopted to remove by-product glycerol during repeated use of the lipase.
Du
Wei
W
Department of Chemical Engineering, Tsinghua University, Beijing 100084, People's Republic of China. duwei@tsinghua.edu.cn
Xu
Yuanyuan
Y
Liu
Dehua
D
D019840:2-Propanol / Q000378:metabolism; D001674:Bioelectric Energy Sources; D001709:Biotechnology / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004789:Enzyme Activation; D004951:Esterification; D005990:Glycerol / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D000432:Methanol / Q000494:pharmacology; D013024:Soybean Oil / Q000737:chemistry / Q000378:metabolism*; D013696:Temperature
D013024:Soybean Oil; D008049:Lipase; D019840:2-Propanol; D005990:Glycerol; D000432:Methanol