Evidence of isozymes for delta6 fatty acid desaturase in rat hepatocytes.
The expression of delta6 fatty acid desaturase, previously identified, was suppressed almost completely by hyper expression of the corresponding antisense gene in a transformant of the rat hepatic cell line BRL-3A. Conversion rates of [1-14C] linoleic acid, alpha-linolenic acid, and tetracosapentaenoic acid into the respective delta6 fatty acids were equivalent to those in control cells. This finding suggested that all of these reactions were catalyzed by at least two delta6 desaturase isozymes in rat hepatocytes.
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, Japan.
Katsuya
K
Inagaki
Tsunehiro
T
Aki
Taketoshi
T
Shiota
Seiji
S
Kawamoto
Seiko
S
Shigeta
Osamu
O
Suzuki
Kazuhisa
K
Ono
D000818:Animals; D016375:Antisense Elements (Genetics) / Q000235:genetics; D015153:Blotting, Western; D002250:Carbon Radioisotopes; D002460:Cell Line; D004281:Docosahexaenoic Acids / Q000378:metabolism; D044943:Fatty Acid Desaturases / Q000235:genetics / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis; D015870:Gene Expression; D022781:Hepatocytes / Q000201:enzymology*; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D019787:Linoleic Acid / Q000378:metabolism; D051381:Rats; D014170:Transformation, Genetic; D017962:alpha-Linolenic Acid / Q000378:metabolism
D016375:Antisense Elements (Genetics); D002250:Carbon Radioisotopes; D005231:Fatty Acids, Unsaturated; D007527:Isoenzymes; D017962:alpha-Linolenic Acid; D004281:Docosahexaenoic Acids; C099830:tetracosapentaenoic acid; D019787:Linoleic Acid; D044943:Fatty Acid Desaturases