Fluid shear contributions to bacteria cell detachment initiated by a monoclonal antibody.
Receptor-mediated adhesion of bacteria to biological surfaces is a significant step leading to infection. Due to an increase in bacterial antibiotic resistance, novel methods to block and disrupt these specific interactions have gained considerable interest as possible therapeutic strategies. Recently, several monoclonal antibodies specific for the Staphylococcus aureus collagen receptor demonstrated specialized ability to displace attached cells from collagen in static assays. In this study, we experimentally examine the monoclonal antibody detachment functionality under physiological shear conditions to evaluate the role of this parameter in the detachment process. The detachment of staphylococci from collagen was quantified in real-time using a parallel plate flow chamber, phase contrast video-microscopy and digital image processing. The results demonstrate a unimodal dependence of detachment on fluid wall shear rate. The observed decrease in effective detachment rate with increasing force at the highest shear levels evaluated is counterintuitive and has not been previously demonstrated. Several possible mechanisms of this result are discussed.
Mascari
Lisa
L
Department of Chemical and Biochemical Engineering, University of Maryland Baltimore County, 1000 Hilltop Circle, ECS 101, Baltimore, Maryland 21250, USA.
Ymele-Leki
Patrick
P
Eggleton
Charles D
CD
Speziale
Pietro
P
Ross
Julia M
JM
D000911:Antibodies, Monoclonal / Q000502:physiology*; D000936:Antigen-Antibody Complex / Q000502:physiology; D001422:Bacterial Adhesion / Q000502:physiology*; D003094:Collagen / Q000502:physiology; D003198:Computer Simulation; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D008954:Models, Biological; D038981:Receptors, Collagen / Q000502:physiology; D012212:Rheology / Q000379:methods*; D033081:Shear Strength; D013211:Staphylococcus aureus / Q000166:cytology* / Q000502:physiology*; D013314:Stress, Mechanical
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D038981:Receptors, Collagen; D003094:Collagen