Saccharification of marine microalgae using marine bacteria for ethanol production.
The saccharification of marine microalgae using amylase from marine bacteria in saline conditions was investigated. An amylase-producing bacterium, Pseudoalterimonas undina NKMB 0074 was isolated and identified. The green microalga NKG 120701 was determined to have the highest concentration of intracellular carbohydrate and was found from our algal culture stocks. P. undina NKMB 0074 was inoculated into suspensions containing NKG 120701 cells and increasingly reduced suspended sugars with incubation time. Terrestrial amylase and glucoamylase were inactive in saline suspension. Therefore, marine amylase is necessary in saline conditions for successful saccharification of marine microalgae.
Matsumoto
Mitsufumi
M
Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-26, Naka-cho, Koganei, Tokyo, Japan. mitsufumi-matsumoto@jpower.co.jp
Yokouchi
Hiroko
H
Suzuki
Nobukazu
N
Ohata
Hiroshi
H
Matsunaga
Tadashi
T
D000681:Amylases / Q000378:metabolism*; D001419:Bacteria / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D000431:Ethanol / Q000378:metabolism*; D056890:Eukaryota / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D007564:Japan; D007700:Kinetics; D010084:Oxidation-Reduction; D012623:Seawater; D013213:Starch / Q000032:analysis / Q000378:metabolism; D014871:Water Microbiology
D000431:Ethanol; D013213:Starch; D000681:Amylases; D005947:Glucose