Overproduction of the Aspergillus niger feruloyl esterase for pulp bleaching application.
A well-known industrial fungus for enzyme production, Aspergillus niger, was selected to produce the feruloyl esterase FAEA by homologous overexpression for pulp bleaching application. The gpd gene promoter was used to drive FAEA expression. Changing the nature and concentration of the carbon source nature (maltose to glucose; from 2.5 to 60 g l(-1)), improved FAEA activity 24.5-fold and a yield of 1 g l(-1) of the corresponding protein in the culture medium was achieved. The secreted FAEA was purified 3.5-fold to homogeneity in a two-step purification procedure with a recovery of 69%. The overproduced protein was characterised and presented properties in good agreement with those of native FAEA. The recombinant FAEA was tested for wheat straw pulp bleaching, with or without a laccase mediator system and xylanase. Best results were obtained using a bi-sequential process with a sequence including xylanase, FAEA and laccase, and yielded very efficient delignification--close to 75%--and a kappa number of 3.9. This is the first report on the potential application of recombinant FAEA in the pulp and paper sector.
Record
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UMR 1163 INRA/Université de Provence de Biotechnologie des Champignons Filamenteux, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL, 163 avenue de Luminy, Case Postale 925, 13288, Marseille Cedex 09, France. record@esil.univ-mrs.fr
Asther
M
M
Sigoillot
C
C
Pagès
S
S
Punt
P J
PJ
Delattre
M
M
Haon
M
M
van den Hondel
C A M J J
CA
Sigoillot
J-C
JC
Lesage-Meessen
L
L
Asther
Marcel
M
D001234:Aspergillus niger / Q000201:enzymology* / Q000235:genetics; D001709:Biotechnology / Q000379:methods; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism; D005822:Genetic Vectors; D007221:Industry; D042845:Laccase / Q000378:metabolism; D010209:Paper; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D014170:Transformation, Genetic; D014908:Triticum / Q000378:metabolism
D011994:Recombinant Proteins; D042845:Laccase; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase; D043364:Endo-1,4-beta Xylanases