A novel cytomedical vehicle capable of protecting cells against complement.
We have developed "Cytomedicine," which consists of functional cells entrapped in semipermeable polymer, and previously reported that APA microcapsules could protect the entrapped cells from injury by cellular immune system. However, microencapsulated cells were not protected from humoral immune system. Here, we developed a novel APA microcapsule, in which APA microbeads (APA(Ba) microbeads) were modified to contain a barium alginate hydrogel within their centers in an attempt to make it more difficult for antibody and complement to permeate the microcapsules. The permeability of APA(Ba) microbeads was clearly less than that of APA microcapsules, presumably due to the presence of barium alginate hydrogel. Cells encapsulated within APA(Ba) microbeads were protected against treatment with xenogeneic anti-serum. Furthermore, murine pancreatic beta-cells encapsulated in APA(Ba) microbeads remained viable and continued to secrete insulin in response to glucose. Therefore, APA(Ba) microbeads may be a useful carrier for developing anti-complement device for cytomedical therapy.
Yoshioka
Yasuo
Y
Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka 565-0871, Japan.
Suzuki
Ryo
R
Oka
Hiroaki
H
Okada
Naoki
N
Okamoto
Takayuki
T
Yoshioka
Tatsunobu
T
Mukai
Yohei
Y
Shibata
Hiroko
H
Tsutsumi
Yasuo
Y
Nakagawa
Shinsaku
S
Miyazaki
Jun-ichi
J
Mayumi
Tadanori
T
D000464:Alginates / Q000737:chemistry*; D000818:Animals; D000910:Antibodies, Heterophile / Q000633:toxicity; D001464:Barium / Q000737:chemistry; D003169:Complement Inactivator Proteins / Q000737:chemistry; D003165:Complement System Proteins / Q000378:metabolism / Q000633:toxicity*; D019610:Cytoprotection; D020100:Hydrogels / Q000737:chemistry; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000378:metabolism; D008567:Membranes, Artificial; D051379:Mice; D008863:Microspheres; D010539:Permeability; D011107:Polylysine / Q000031:analogs & derivatives* / Q000737:chemistry*; D014407:Tumor Cells, Cultured
D000464:Alginates; D000910:Antibodies, Heterophile; D003169:Complement Inactivator Proteins; D020100:Hydrogels; D007328:Insulin; D008567:Membranes, Artificial; C051546:alginate-polylysine-alginate; D001464:Barium; D011107:Polylysine; D003165:Complement System Proteins