Covalent immobilization of oligonucleotides on p-aminophenyl-modified carbon screen-printed electrodes for viral DNA sensing.
DNA-sensing platforms were prepared by covalently attaching oligonucleotide capture probes onto p-aminophenyl-functionalized carbon surfaces and applied to the determination of an amplified herpes virus DNA sequence in an electrochemical hybridization assay.
Laboratoire de Microbiologie Médicale et Moléculaire, Faculté de Médecine, 7 Boulevard Jeanne d'Arc, 21000 Dijon, France.
Audrey
A
Ruffien
Murielle
M
Dequaire
Pierre
P
Brossier
D000627:Aminophenols / Q000737:chemistry*; D001483:Base Sequence; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D002244:Carbon / Q000737:chemistry*; D003587:Cytomegalovirus / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004279:DNA, Viral / Q000032:analysis* / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D006801:Humans; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013499:Surface Properties
D000627:Aminophenols; D004279:DNA, Viral; D015345:Oligonucleotide Probes; D009841:Oligonucleotides; D002244:Carbon