Ras transgene expression in TG-AC mice treated with benzo[a]pyrene.
Transgene expression and skin tumorigenicity were investigated in transgenic TG-AC mice carrying the v-Ha-ras after treatment with benzo[a]pyrene (BP). Animals treated with 40 microg BP (x2/week/mouse) showed 100% tumor response after 25 weeks, as did 40% of the mice treated with 20 microg BP but 10 microg BP did not produce a tumor response. In the case of animals treated with 40 microg BP for 25 weeks, most of the tumors were proven to be carcinomas (80%, 4 out of 5 mice), and all tumors were shown to be positive in terms of transgene expression detected by in situ hybridization. These data suggest that BP was tumorigenic in a dose-dependent manner in TG-AC mice and that TG-AC mice were dependent on transgene expression during BP carcinogenesis.
Division of Toxicology, College of Pharmacy, SungKyunKwan University, Chunchun-dong, Suwon, Kyonggi-do, South Korea. bmlee@skku.ac.kr
Byung Mu
BM
Lee
Hyung Sik
HS
Kim
In Sun
IS
Kim
Mi Young
MY
Ahn
Sun Ah
SA
Chun
Sun Dong
SD
Yoo
Yo An
YA
Park
Sul Min
SM
Choi
Kyu Bong
KB
Kim
Soon Young
SY
Han
Kui Lae
KL
Park
Seung Jun
SJ
Kwack
Hyun Sue
HS
Song
Kyung Rim
KR
Lee
Hye Jin
HJ
Bae
Myung Haing
MH
Cho
D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D002273:Carcinogens / Q000633:toxicity*; D002277:Carcinoma / Q000139:chemically induced; D005260:Female; D017403:In Situ Hybridization; D051379:Mice; D008822:Mice, Transgenic; D012878:Skin Neoplasms / Q000139:chemically induced; D019076:Transgenes; D018631:ras Proteins / Q000096:biosynthesis* / Q000235:genetics
D002273:Carcinogens; D001564:Benzo(a)pyrene; D018631:ras Proteins