A new sterol sulfate, Sch 572423, from a marine sponge, Topsentia sp.
Bioassay-guided fractionation of an active fraction from a marine sponge Topsentia sp. in our marine fraction library (MFL) led to the isolation and identification of halistanol sulfate (1) and a new sterol sulfate Sch 572423 (2). Compounds 1 and 2 were identified as P2Y(12) inhibitors with IC(50) of 0.48 and 2.2 microM, respectively. The general method of purification for the MFL library and the structure elucidation of compound 2 are described.
Yang
Shu-Wei
SW
Schering-Plough Research Institute, 2015 Galloping Hill Road, Kenilworth, NJ 07033, USA. shu-wei.yang@spcorp.com
Buivich
Alexei
A
Chan
Tze-Ming
TM
Smith
Michelle
M
Lachowicz
Jean
J
Pomponi
Shirley A
SA
Wright
Amy E
AE
Mierzwa
Ronald
R
Patel
Mahesh
M
Gullo
Vincent
V
Chu
Min
M
D000818:Animals; D008565:Membrane Proteins / Q000037:antagonists & inhibitors; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D011161:Porifera / Q000737:chemistry*; D058919:Purinergic P2 Receptor Antagonists; D058925:Receptors, Purinergic P2Y12; D013261:Sterols / Q000737:chemistry* / Q000302:isolation & purification*; D013329:Structure-Activity Relationship
D008565:Membrane Proteins; D058919:Purinergic P2 Receptor Antagonists; D058925:Receptors, Purinergic P2Y12; C480884:Sch 572423; D013261:Sterols; C072244:halistanol trisulfate