[Estrogenic activity of some environmental chemicals].
The study was designed to explore the estrogenic activity of environmental disrupters [n-4-noniphenol (NP), bisphenol A (bisA) and dibutylphthalate (DBP)]. Estrogen-respective breast cancer cell line T47D was grown in DMEM medium containing 10% bovine serum. Five days before the addition of the test compounds, the cell were washed in phosphate-buffered saline, and the medium was substituted with a phenol red-free DMEM medium containing 5% dextran charcoal-stripped FBS. The respective test compound was added in fresh medium and the control cell received only the vehicle (ethanol). The proliferation of T47D cells was analyzed by MTT assay, 3H-TdR incorporation assay and flow sytometer. The results indicated that, compared with the EtOH control cell, the test compounds (n-4-noniphenol, Bisphenol A and dibutylphthalate), like estradiol, markedly enhanced the proliferation of T47D cell and the metaphase of cell division, and the results showed time-dependent and dose-dependent model. These data showed that the tested chemicals could enhance the proliferation of human cervix cancer cell in vitro. This might hinted that these chemicals possessed estrogenic activity and they might play their estrogen through estrogenic receptor.
Huaxi School of Public Health, Sichuan University, Chengdu 610041, China.
Zengli
Z
Yu
Lishi
L
Zhang
Desheng
D
Wu
D001559:Benzhydryl Compounds; D001943:Breast Neoplasms / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D003993:Dibutyl Phthalate / Q000633:toxicity; D004785:Environmental Pollutants / Q000633:toxicity*; D004968:Estrogens, Non-Steroidal / Q000633:toxicity*; D006801:Humans; D010636:Phenols / Q000633:toxicity; D014407:Tumor Cells, Cultured; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D003993:Dibutyl Phthalate; D004785:Environmental Pollutants; D004968:Estrogens, Non-Steroidal; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds