A strategy for mapping the heterochromatin of chromosome 2 of Drosophila melanogaster.
The heterochromatin of chromosome 2 of Drosophila melanogaster has been among the best characterized models for functional studies of heterochromatin owing to its abundance of genetic markers. To determine whether it might also provide a favorable system for mapping extended regions of heterochromatin, we undertook a project to molecularly map the heterochromatin of the left arm of chromosome 2 (2Lh). In this paper, we describe a strategy that used clones and sequence information available from the Drosophila Genome Project and chromosome rearrangements to construct a map of the distal most portion of 2Lh. We also describe studies that used fluorescent in situ hybridization (FISH) to examine the resolution of this technique for cytologically resolving heterochromatic sequences on mitotic chromosomes. We discuss how these mapping studies can be extended to more proximal regions of the heterochromatin to determine the structural patterns and physical dimensions of 2Lh and the relationship of structure to function.
Department of Zoology, University of Washington, Box 351800, Seattle, WA 98195, USA.
Jiro C
JC
Yasuhara
Marcella
M
Marchetti
Laura
L
Fanti
Sergio
S
Pimpinelli
Barbara T
BT
Wakimoto
D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping / Q000379:methods*; D022202:Chromosomes, Artificial, Bacterial; D030561:Databases, Nucleic Acid; D004331:Drosophila melanogaster / Q000235:genetics*; D006570:Heterochromatin / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D015183:Restriction Mapping
D006570:Heterochromatin