[Measurement of the reactive oxygen species and cytokines in the seminal plasma of leukocytospermic patients].
OBJECTIVE
To detect the levels of reactive oxygen species (ROS), superoxide dismutase(SOD) and interleukin 8(IL-8) in seminal plasma of infertile patients, and evaluate the possible relationship between those levels.


METHODS
Semen was collected from normal donors (15 cases), infertile men without infection (16 cases), and infertile men with infection (leukocytospermia, 11 cases). The routine analysis of semen was accomplished, and then the levels of IL-8, malondialdehyde (MDA), SOD, and white blood cell (WBC) were examined. The correlative analysis between the level of ROS and other parameters in these populations was made.


RESULTS
In leukocytospermic group, the levels of MDA, WBC, and IL-8 were higher than those in the other two groups (P < 0.001). Significantly positive correlation was observed between IL-8 and MDA (r = 0.852, P < 0.001) and between the levels of IL-8 and WBC.


CONCLUSIONS
These findings suggest that increased oxidative stress in patients with leukocytospermia may cause the increase of IL-8(r = 0.818, P < 0.01). The increased oxidative stress may be due to defect in ROS scavenging system.
Liu
Jing
J
Department of Urology, Zhongda Hospital Affiliated to Medical College of Southeast University, Nanjing, Jiangsu 210009, China.
Wang
Yi-Xing
YX
Wu
Yu-Lun
YL
Qian
Xian-Ming
XM
Xiang
Zu-Qiong
ZQ
D000328:Adult; D016207:Cytokines / Q000097:blood*; D006801:Humans; D007248:Infertility, Male / Q000097:blood* / Q000150:complications; D008297:Male; D052801:Male Urogenital Diseases / Q000097:blood / Q000150:complications; D017382:Reactive Oxygen Species / Q000097:blood*; D012661:Semen
D016207:Cytokines; D017382:Reactive Oxygen Species