Infection of Mongolian gerbils with Chinese Helicobacter pylori strains.
To date only a few Helicobacter pylori strains have been demonstrated to colonise Mongolian gerbils successfully. The aim of this study was to establish stable colonisation of Chinese strains of H. pylori in gerbils. Fresh clinical isolates from Chinese patients were inoculated into gerbils. At 4-6 weeks post inoculation, infection status was evaluated by culture, biopsy urease test and pathology. Sequencing of glmM and random amplified polymorphic DNA (RAPD) fingerprinting of DNA from cultured H. pylori were used to evaluate the genetic identity of pre-inoculated and post-inoculated strains. The ability of pre- and post-inoculated strains to stimulate interleukin-8 transcription in L5F11 gastric epithelial cells was analysed. Three of five clinical isolates colonised gerbils. The three pre- and post-inoculation strains had identical glmM sequences and RAPD profiles, and stimulated luciferase secretion from L5F11 epithelial cells. The strain that caused severe pathological changes was selected for repeat infection to prove reproducible and stable colonisation. The cagA+ strain 42GX gave stable colonisation in the gerbil and induced severe gastritis.
Molecular Medicine Unit, Level 7 Clinical Sciences Building, St. James's University Hospital, Leeds LS9 7TF, UK.
Jingtong
J
Wang
Michelle
M
Court
Anthony H T
AH
Jeremy
Mohamed A
MA
Aboshkiwa
Philip A
PA
Robinson
Jean E
JE
Crabtree
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004195:Disease Models, Animal; D005753:Gastric Mucosa / Q000166:cytology / Q000382:microbiology; D005756:Gastritis / Q000382:microbiology / Q000503:physiopathology*; D005849:Gerbillinae; D016481:Helicobacter Infections / Q000382:microbiology / Q000503:physiopathology*; D016480:Helicobacter pylori / Q000145:classification / Q000235:genetics / Q000472:pathogenicity*; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis; D008969:Molecular Sequence Data; D010733:Phosphoglucomutase / Q000737:chemistry / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA
D016209:Interleukin-8; C097631:phosphoglucosamine mutase; D010733:Phosphoglucomutase