[The influence of pregnancy and hormone on the lymphatic stomata in guinea pig's ovary bursa].
In order to study the influence of pregnancy and hormone on the lymphatic stomata of guinea pig's ovary bursa, the lymphatic stomata of ovary bursa were observed during pregnancy and by using exogenous hormone, including serum gonadotrophin (PMSG) combined with human chorionic gonadotropin (HCG) to promote ovulation and androgen to inhibit the ovary. Then the lymphatic stomata in the inner layer of ovary bursa and their absorption functions were observed with scanning electron microscope (SEM) and trypan blue as a tracer respectively. The lymphatic stomata were also counted by the Elescope computer image processing system. We found that the quantity of tracer absorbed by the lymphatic stomata of ovary bursa in pregnant group was more than that in ovulation-promoted group, and least in the androgen-treated group, which had statistical significance between each couples of them (P < 0.05). The opening area of the lymphatic stomata in pregnant, ovulation-promoted and androgen-treated groups were 189.9 +/- 48.7 micron 2/1000 micron 2, 104.4 +/- 31.2 micron 2/1000 micron 2 and 40.5 +/- 18.7 micron 2/1000 micron 2 respectively, which had remarkable statistical difference between each couples (P < 0.01). Under SEM observation, the secretory granules in ciliated columnar epithelium were less in pregnant group than the other two, while both the secretory granules and the cilium were most active in ovulation-promoted group. The results indicated that both the openings and absorption functions of the lymphatic stomata in guinea pig's ovary bursa could be affected by pregnancy and exogenous homone. There existed relationship between ovary function and the regulation of the lymphatic stomata in ovary bursa.
Institute of Cell Biology & Tissue Engineering, Department of Lymphology, Zhejiang University, Hangzhou 310031.
Mei Hua
MH
Sui
Ji Cheng
JC
Li
D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D004338:Drug Combinations; D005260:Female; D006064:Gonadotropins, Equine / Q000494:pharmacology; D006168:Guinea Pigs; D006728:Hormones / Q000494:pharmacology*; D008208:Lymphatic System / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D010053:Ovary / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000502:physiology*; D013739:Testosterone / Q000031:analogs & derivatives* / Q000494:pharmacology
D006063:Chorionic Gonadotropin; D004338:Drug Combinations; D006064:Gonadotropins, Equine; D006728:Hormones; C036835:PMSG-HCG; D013739:Testosterone; C040594:testosterone acetate