Rhesus rhadinovirus infection in healthy and SIV-infected macaques at Tulane National Primate Research Center.
Rhesus rhadinovirus (RRV) infection was quantified in peripheral blood mononuclear cells (PBMC) from healthy and simian immunodeficiency virus (SIV)-infected rhesus macaques (Macaca mulatta) at the Tulane National Primate Research Center and in a large collection of simian acquired immunodeficiency syndrome--(SAIDS)-associated lymphomas. Quantification of RRV load was performed by real-time PCR using amplification primers specific for the RRV interleukin-6 homologue (RRV vIL-6). RRV infection was detected infrequently and at low levels in PBMC of randomly selected healthy animals. Examination of longitudinally collected PBMC from 22 SIV-infected animals throughout progression to SAIDS revealed similarly low RRV loads that sometimes increased with advancing disease. RRV infection was detected more frequently in the peripheral blood of SIV-infected animals than in healthy animals. Examination of SAIDS-associated lymphomas showed that RRV is rare within the tumor mass, likely representing infection in an occasional tumor-infiltrating cell or contaminating blood. The results indicate that RRV infection in PBMC is not predictive of, and is apparently not required for, development of lymphoma or hyperplastic lymphadenopathy in SIV-infected animals at TNPRC.
Department of Microbiology and Immunology, Program in Molecular Pathogenesis and Immunity, Tulane Cancer Center, Tulane University Health Sciences Center, New Orleans, LA 70112, USA.
K
K
Ruff
G B
GB
Baskin
L
L
Simpson
M
M
Murphey-Corb
L S
LS
Levy
D000818:Animals; D004279:DNA, Viral / Q000378:metabolism; D005260:Female; D006566:Herpesviridae Infections / Q000821:virology*; D008137:Longitudinal Studies; D008223:Lymphoma / Q000821:virology; D008253:Macaca mulatta / Q000821:virology*; D008297:Male; D008992:Monkey Diseases / Q000821:virology*; D016133:Polymerase Chain Reaction; D018144:Rhadinovirus; D016097:Simian Acquired Immunodeficiency Syndrome / Q000150:complications / Q000821:virology*; D015302:Simian Immunodeficiency Virus; D014412:Tumor Virus Infections / Q000821:virology*; D019562:Viral Load
D004279:DNA, Viral