Structure and biological activities of beta-glucans from yeast and mycelial forms of Candida albicans.
We have achieved the extraction of cell wall beta-glucan from the mycelial form of Candida albicans (C. albicans) IFO 0579 (M-CSBG) by using acetic acid, sodium hypochlorite (NaClO), and dimethylsulfoxide (DMSO) treatments. The yield of M-CSBG was significantly lower (7.5% from dried mycelial cells) than that of the yeast form from C. albicans IFO 1385 (Y-CSBG, 25.9% from dried yeast cells). The properties of M-CSBG were similar to those of Y-CSBG in terms of nuclear magnetic resonance (NMR) spectra and limulus reactivity. Molecular weight (Mw) of M-CSBG was slightly higher than that of Y-CSBG. Both Y-CSBG and M-CSBG induced the production of comparable amounts of macrophage inflammatory protein-2 (MIP-2), a chemotactic factor, from mouse peritoneal exudate cells (PEC) in vitro. These findings suggest that the structure and properties of CSBG from yeast and mycelial cells are similar to each other.
Miura
Noriko N
NN
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo 192-0392, Japan. miuranno@ps.toyaku.ac.jp
Adachi
Yoshiyuki
Y
Yadomae
Toshiro
T
Tamura
Hiroshi
H
Tanaka
Shigenori
S
Ohno
Naohito
N
D000818:Animals; D002176:Candida albicans / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D002478:Cells, Cultured; D054426:Chemokine CXCL2; D005936:Glucans / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D008033:Limulus Test / Q000379:methods; D051379:Mice; D008809:Mice, Inbred C3H; D015846:Monokines / Q000096:biosynthesis; D025282:Mycelium / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D015003:Yeasts / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism
D054426:Chemokine CXCL2; D005936:Glucans; D015846:Monokines