ATP leakage from yeast cells treated by extracellular glycolipids of Pseudozyma fusiformata.
The ustilaginaceous yeast Pseudozyma fusiformata secreted glycolipids which were lethal to many yeasts and fungi more active at pH of about 4.0, and in the temperature range of 20-30 degrees C. Purified glycolipids enhanced non-specific permeability of the cytoplasmic membrane in sensitive cells, which resulted in ATP leakage and susceptibility of the cells to staining with bromocresol purple. Cells of Saccharomyces cerevisiae lost the ability to acidify the medium. Basidiomycetous yeasts were more sensitive to the glycolipids than ascomycetous ones. The minimal effective glycolipid concentration was 0.13 and 0.26 mg ml(-1) for Cryptococcus terreus and Filobasidiella neoformans, while for Candida albicans and Saccharomyces cerevisiae it was 1.0 and 1.6 mg ml(-1).
G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Moscow region, Pushchino, Russia. alla@ibpm.serpukhov.su
Tatiana V
TV
Kulakovskaya
Ekaterina V
EV
Kulakovskaya
Wladyslav I
WI
Golubev
D000255:Adenosine Triphosphate / Q000378:metabolism*; D001962:Bromcresol Purple / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D005110:Extracellular Space; D006017:Glycolipids / Q000378:metabolism / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents / Q000378:metabolism; D014587:Ustilaginales / Q000737:chemistry* / Q000378:metabolism; D015003:Yeasts / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism
D006017:Glycolipids; D007202:Indicators and Reagents; D001962:Bromcresol Purple; D000255:Adenosine Triphosphate