Activity, conformation and dynamics of cutinase adsorbed on poly(methyl methacrylate) latex particles.
The adsorption of a recombinant cutinase from Fusarium solani pisi onto the surface of 100 nm diameter poly(methyl methacrylate) (PMMA) latex particles was evaluated. Adsorption of cutinase is a fast process since more than 70% of protein molecules are adsorbed onto PMMA at time zero of experiment, irrespective of the tested conditions. A Langmuir-type model fitted both protein and enzyme activity isotherms at 25 degrees C. Gamma(max) increased from 1.1 to 1.7 mg m(-2) and U(max) increased from 365 to 982 U m(-2) as the pH was raised from 4.5 to 9.2, respectively. A decrease (up to 50%) in specific activity retention was observed at acidic pH values (pH 4.5 and 5.2) while almost no inactivation (eta(act) congruent with 87-94%) was detected upon adsorption at pH 7.0 and 9.2. Concomitantly, far-UV circular dichroism (CD) spectra evidenced a reduction in the alpha-helical content of adsorbed protein at acidic pH values while at neutral and alkaline pH the secondary structure of adsorbed cutinase was similar to that of native protein. Fluorescence anisotropy decays showed the release of some constraints to the local motion of the Trp69 upon protein adsorption at pH 8.0, probably due to the disruption of the tryptophan-alanine hydrogen bond when the tryptophan interacts with the PMMA surface. Structural data associated with activity measurements at pH 7.0 and 9.2 showed that cutinase adsorbs onto PMMA particles in an end-on orientation with active site exposed to solvent and full integrity of cutinase secondary structure. Hydrophobic interactions are likely the major contribution to the adsorption mechanism at neutral and alkaline pH values, and a higher amount of protein is adsorbed to PMMA particles with increasing temperature at pH 9.2. The maximum adsorption increased from 88 to 140 mg cutinase per g PMMA with temperature raising from 25 to 50 degrees C, at pH 9.2.
Baptista
R P
RP
Centro de Engenharia Biológica e Quïmica, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001 Lisbon, Portugal.
Santos
A M
AM
Fedorov
A
A
Martinho
J M G
JM
Pichot
C
C
Elaïssari
A
A
Cabral
J M S
JM
Taipa
M A
MA
D002265:Carboxylic Ester Hydrolases / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000737:chemistry; D004926:Escherichia coli / Q000737:chemistry / Q000201:enzymology / Q000235:genetics; D005670:Fusarium / Q000737:chemistry* / Q000201:enzymology* / Q000235:genetics; D008863:Microspheres; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012645:Selenomethionine / Q000031:analogs & derivatives*; D013696:Temperature
D004800:Enzymes, Immobilized; D011994:Recombinant Proteins; C069627:adenosylselenomethionine; D019904:Polymethyl Methacrylate; D012645:Selenomethionine; D002265:Carboxylic Ester Hydrolases; C019952:cutinase