Active nuclear shuffling system using a swollen conidium of Trichoderma reesei.
Cellulase hyperproducers of Trichoderma reesei can be constructed using autopolyploidization and haploidization techniques. To increase the efficiency of this method, the active nuclear shuffling system in a swollen conidium was effective. A dried mature green conidium of a model strain, T. reesei QM6a (IFO 31326), was swollen to make room for a larger autopolyploid nucleus. After colchicine treatment, a larger autopolyploid nucleus was produced in such a swollen conidium. Benomyl treatment of swollen conidia generated multiple smaller nuclei from one larger autopolyploid nucleus. Those smaller nuclei were transported through conidia to mycelia after germination. This system could contribute to increasing the efficiency of genetic shuffling.
Toyama
Hideo
H
Department of Food Science and Technology, Faculty of Horticulture, Minamikyushu University, Miyazaki 884-0003, Japan. gaf00771@nifty.com
Yano
Makiko
M
Gisushi
Akane
A
Hotta
Takeshi
T
Toyama
Nobuo
N
D002480:Cellulase / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D009172:Mycology / Q000379:methods; D014242:Trichoderma / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development
D002480:Cellulase