Presence of glyoxylate cycle enzymes in the mitochondria of Euglena gracilis.
Isocitrate lyase and malate synthase are specific enzymes of the glyoxylate cycle, used here as glyoxysomal markers. Both enzymes were found in the mitochondrial fraction after organelle fractionation by isopycnic centrifugation. Electron microscopy of this fraction indicated that mitochondria were the only recognizable organelles. Using an immunogold labeling method with anti-(malate synthase) antiserum, the only organelles stained in cells were the mitochondria. These results show that the glyoxylate cycle is present in mitochondria in Euglena.
Department of Food and Nutrition, Gifu City Women's College, Gifu, Gifu 501-0192, Japan. kono@gifu-cwc.ac.jp
Kouki
K
Ono
Maki
M
Kondo
Tetsuaki
T
Osafune
Kazutaka
K
Miyatake
Hiroshi
H
Inui
Shozaburo
S
Kitaoka
Mikio
M
Nishimura
Yoshihisa
Y
Nakano
D000818:Animals; D002500:Centrifugation, Isopycnic; D005056:Euglena gracilis / Q000201:enzymology* / Q000648:ultrastructure; D006038:Glyoxylates / Q000378:metabolism*; D007150:Immunohistochemistry; D007522:Isocitrate Lyase / Q000378:metabolism*; D008292:Malate Synthase / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology* / Q000648:ultrastructure
D006038:Glyoxylates; D008292:Malate Synthase; D007522:Isocitrate Lyase