Characterization of a new Pseudomonas aeruginosa strain NJ-15 as a potential biocontrol agent.
Phylogenetic characterization of soil isolate NJ-15, based on sequence homology of a partial 746-bp fragment of 16SrDNA amplicon, with the ribosomal database sequences (http://www.msu.edu/RDP/cgis/phylip.cgi), validated the strain as Pseudomonas aeruginosa. The strain NJ-15 produced a substantial amount of indole acetic acid (IAA) in tryptophan-supplemented medium. Besides, the strain also exhibited significant production of both the siderophore and hydrogen cyanide (HCN) on chrome azurol S and King's B media, respectively. The data revealed lower HCN production under iron-limiting conditions vis-à-vis higher HCN release with iron stimulation. Significant growth inhibition of phytopathogenic fungi occurred in the order as Fusarium oxysporum > Trichoderma herizum > Alternaria alternata > Macrophomina phasiolina upon incubation with strain NJ-15 cells. Thus, the secondary metabolites producing new Pseudomonas aeruginosa strain NJ-15 exhibited innate potential of plant growth promotion and biocontrol activities in vitro.
Bano
Nazneen
N
Department of Agricultural Microbiology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh-202002, India.
Musarrat
Javed
J
D000898:Antibiosis; D001483:Base Sequence; D003470:Culture Media; D004275:DNA, Ribosomal / Q000032:analysis; D005658:Fungi / Q000254:growth & development*; D006856:Hydrogen Cyanide / Q000378:metabolism; D007210:Indoleacetic Acids / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D008969:Molecular Sequence Data; D010572:Pest Control, Biological; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology; D010937:Plant Growth Regulators / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D017262:Siderophores / Q000378:metabolism; D012988:Soil Microbiology
D003470:Culture Media; D004275:DNA, Ribosomal; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D012336:RNA, Ribosomal, 16S; D017262:Siderophores; D006856:Hydrogen Cyanide; C030737:indoleacetic acid; D007501:Iron