Identification and nucleotide sequence analysis of a cryptic plasmid, pRM21, from Rhodothermus marinus.
Here we report the identification and nucleotide sequence analysis of pRM21, a plasmid isolated from the thermophilic eubacterium Rhodothermus marinus. pRM21 consists of 2935 bp, has a G+C content of 58.2% and one major open reading frame whose deduced product shows significant similarities to RepA proteins from several plasmids, the highest being to the RepA of pSa from Escherichia coli. A region with the characteristics of iteron-containing replicons, three 19 bp repeats, DnaA boxes, an A+T rich region and GATC sequences, was identified. Of 40 additional R. marinus strains screened for plasmids, six (15%) were found to harbour plasmids with the same size and restriction pattern as pRM21.
Laboratory of Molecular Genetics, Institute of Biology, University of Iceland, Grensasvegur 12, IS-108 Reykjavik, Iceland.
Sveinn
S
Ernstsson
Snaedis H
SH
Bjornsdottir
Zophonias O
ZO
Jónsson
Sigrídur H
SH
Thorbjarnardottir
Gudmundur
G
Eggertsson
Astridur
A
Palsdottir
D020856:AT Rich Sequence; D001419:Bacteria / Q000235:genetics*; D001483:Base Sequence; D004265:DNA Helicases; D004268:DNA-Binding Proteins; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D011506:Proteins / Q000235:genetics; D012093:Replicon / Q000235:genetics; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D015534:Trans-Activators
D004268:DNA-Binding Proteins; D011506:Proteins; D015534:Trans-Activators; C045264:replication initiator protein; D004265:DNA Helicases