Scatter factor regulation of integrin expression and function on oral epithelial cells.
Integrin receptors and the growth factor, scatter factor (SF; also known as hepatocyte growth factor) have been shown to modulate similar cellular processes including embryogenesis, wound healing and tumour invasion. The purpose of this study was to examine the role of SF in the regulation of integrin expression, migration and adhesion in normal human oral keratinocytes (NHK). Integrin expression was examined using flow cytometry, SF did not alter levels of expression but had a dramatic effect on cell morphology, inducing migratory filopodia and lamellipodia. SF selectively induced migration towards fibronectin, but not towards collagen I. Integrin function was further investigated by measuring the ability of NHK to adhere and migrate on various integrin ligands. SF reduced adhesion of NHK to collagen types I and IV, laminins 1 and 5 and fibronectin. The inclusion of function-blocking antibodies revealed that SF mediated upregulation of migration through alpha(5)beta(1) integrin and downregulation of adhesion through alpha(v) integrins. SF increased tyrosine phosphorylation of FAK protein in NHK after a 30-min treatment. These results show that SF can affect keratinocyte behaviour by modifying integrin function but not expression.
Department of Oral and Maxillofacial Pathology, Eastman Dental Institute for Oral Health Care Sciences, University College London, 256 Gray's Inn Road, WC1X 8LD, London, UK.
Sopee
S
Poomsawat
Simon A
SA
Whawell
Matthew J
MJ
Morgan
Gareth J
GJ
Thomas
Paul M
PM
Speight
D000906:Antibodies / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D017228:Hepatocyte Growth Factor / Q000494:pharmacology / Q000502:physiology*; D006801:Humans; D016023:Integrins / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D009061:Mouth Mucosa / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D011994:Recombinant Proteins / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects
D000906:Antibodies; D016023:Integrins; D011994:Recombinant Proteins; D017228:Hepatocyte Growth Factor