[Combined effects of lead and ethanol on rat sperms].
In order to study the combined effects of lead and ethanol on rat sperm, 54 male rats were divided into 9 groups randomly. One group was given distilled water as control, and the others (A-H) groups were given lead and ethanol at the concentrations of lead 11 mg/kgBW and lead 22 mg/kgBW, ethanol 0.9 g/kgBW, ethanol 1.8 g/kgBW, lead 11 mg/kgBW + ethanol 0.9 g/kgBW, lead 11 mg/kgBW + ethanol 1.8 g/kgBW, lead 22 mg/kgBW + ethanol 0.9 g/kgBW, lead 22 mg/kgBW + ethanol 1.8 g/kgBW in stomachs respectively for four weeks. The rats were sacrificed by the end of the fourth week. Epididymis were isolated and examined for sperm motility, counts and morphology. The results showed that the spermatozoa motility as well as spermatozoa count was lower; the spermatozoa morphological changed and blood lead level and ratio of epididymis to weight were higher in all combined experimental groups than those in groups with lead or ethanol alone. ANOVA analysis indicated that there were interaction between lead and ethanol on above indices (P < 0.05 P < 0.01). The multiple linear regression showed that there was linear correlation between blood lead and spermatozoa motility, spermatozoa morphological change. It is concluded that there were notable combined effects of lead and ethanol on semen quality in male rats.
School of Public Health, Shandong Medical University, Jinan 250012, China.
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Yu
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Bai
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Li
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Wang
D000818:Animals; D004357:Drug Synergism; D004822:Epididymis / Q000033:anatomy & histology / Q000187:drug effects; D000431:Ethanol / Q000633:toxicity*; D007854:Lead / Q000633:toxicity*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012044:Regression Analysis; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects*
D007854:Lead; D000431:Ethanol