[Effect of homocysteine on the proliferation and differentiation of rat embryo midbrain cell].
In order to determine the potential mechanisms by which HCY may exerts its teratogenic effects, a micromass culture for rat embryo midbrain cell was used to reveal the differentiation and proliferation of the cell under different HCY doses (0-10 mmol/L). The results showed that with increased concentration of HCY, the promotion effect changed into inhibition. The concentration inhibiting the formation of differentiated foci by 50% of the control was 4.3 mmol/L, and 2.3 mmol/L when S9 was added. It had an inhibition effects on proliferation in the testing scope. The result suggested that HCY obviously inhibited more cell differentiation than proliferation. The teratogenic hazard of HCY may play an important role in the occurrence of neural tube defects.
National Center of Maternal and Infant Health, Beijing Medical University, Beijing 100083, China.
H
H
Liu
Y
Y
Li
H
H
Ye
Z
Z
Zhao
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005260:Female; D006710:Homocysteine / Q000633:toxicity*; D008636:Mesencephalon / Q000166:cytology*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013723:Teratogens / Q000633:toxicity*
D013723:Teratogens; D006710:Homocysteine