Vasospasm following subarachnoid hemorrhage: evidence against functional upregulation of protein kinase C constrictor pathway.
This study tested the hypothesis that vasospasm due to subarachnoid hemorrhage involves the functional upregulation of protein kinase C. Spasm of the rabbit basilar artery was achieved using a double hemorrhage model, which we previously demonstrated was endothelin-1 dependent. In situ effects of agents were determined by direct measurement of vessel diameter following their suffusion in a cranial window. Chelerythrine, a protein kinase C inhibitor, relaxed the spasm. However, relaxations to chelerythrine were not significantly greater in endothelin-1 constricted spastic vessels initially relaxed with the endothelin converting enzyme inhibitor, phosphoramidon, as compared to endothelin-1 constricted control vessels. These results suggest that subarachnoid hemorrhage induced vasospasm does not involve functional upregulation of protein kinase C.
Yoon
SeongHun
S
Department of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine, PO Box 670575, Cincinnati, OH 45267-0575, USA.
Zuccarello
Mario
M
Rapoport
Robert M
RM
D000470:Alkaloids; D000818:Animals; D001488:Basilar Artery / Q000187:drug effects / Q000378:metabolism; D053119:Benzophenanthridines; D019332:Endothelin-1 / Q000378:metabolism / Q000494:pharmacology; D004791:Enzyme Inhibitors; D006020:Glycopeptides / Q000494:pharmacology; D008297:Male; D010617:Phenanthridines / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism*; D011817:Rabbits; D013345:Subarachnoid Hemorrhage / Q000150:complications* / Q000201:enzymology; D015854:Up-Regulation; D014661:Vasoconstriction / Q000187:drug effects*; D020301:Vasospasm, Intracranial / Q000201:enzymology* / Q000209:etiology*
D000470:Alkaloids; D053119:Benzophenanthridines; D019332:Endothelin-1; D004791:Enzyme Inhibitors; D006020:Glycopeptides; D010617:Phenanthridines; D011480:Protease Inhibitors; C016299:chelerythrine; D011493:Protein Kinase C; C008890:phosphoramidon