Allele-specific differential expression of a common adiponectin gene polymorphism related to obesity.
Adiponectin gene polymorphisms have recently been reported to be associated with obesity, insulin sensitivity, and the risk of type 2 diabetes. We examined a T94G polymorphism of the adiponectin gene in 245 ostensibly normal nondiabetic subjects. The G allele frequency was lower among subjects with higher BMI (> or =27) than in those with lower BMI. BMI was inversely correlated with the dose of G allele. Multivariate linear regression analyses showed that the adiponectin genotypes were significantly related to BMI after adjusting for age and gender. The dose of the G allele was associated with a reduction of approximately 1.12 kg/m(2) in BMI. We further found that the relative mRNA levels of G allele were consistently higher than those of T allele in the omental adipose tissue from 21 heterozygous subjects. Finally, we observed that the expression levels of adiponectin affected insulin-stimulated glucose uptake in differentiated 3T3-L1 adipocytes. In conclusion, the allele-specific differential expression of this common polymorphism could be responsible for its biological effects observed in this and the other studies.
Division of Endocrinology and Metabolism, Department of Internal Medicine, National Taiwan University Hospital, and Graduate Institute of Clinical Medicine, National Taiwan University Medical College, Taipei, Taiwan.
Wei-Shiung
WS
Yang
Pei-Ling
PL
Tsou
Wei-Jei
WJ
Lee
Da-Lun
DL
Tseng
Chi-Ling
CL
Chen
Chi-Chung
CC
Peng
Kuan-Ching
KC
Lee
Mei-Ju
MJ
Chen
Chang-Jen
CJ
Huang
Tong-Yuan
TY
Tai
Lee-Ming
LM
Chuang
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism*; D052242:Adiponectin; D000328:Adult; D000483:Alleles; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D015992:Body Mass Index; D005260:Female; D015870:Gene Expression; D005951:Glucose Tolerance Test; D006801:Humans; D007328:Insulin / Q000494:pharmacology / Q000502:physiology; D007333:Insulin Resistance; D036341:Intercellular Signaling Peptides and Proteins; D008297:Male; D051379:Mice; D008875:Middle Aged; D009765:Obesity / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics* / Q000378:metabolism
D052242:Adiponectin; D001786:Blood Glucose; D007328:Insulin; D036341:Intercellular Signaling Peptides and Proteins; D011506:Proteins