NMDA receptor trafficking through an interaction between PDZ proteins and the exocyst complex.
NMDA (N-methyl-D-aspartate) receptors (NMDARs) are targeted to dendrites and anchored at the post-synaptic density (PSD) through interactions with PDZ proteins. However, little is known about how these receptors are sorted from the endoplasmic reticulum and Golgi apparatus to the synapse. Here, we find that synapse-associated protein 102 (SAP102) interacts with the PDZ-binding domain of Sec8, a member of the exocyst complex. Our results show that interactions between SAP102 and Sec8 are involved in the delivery of NMDARs to the cell surface in heterologous cells and neurons. Furthermore, they suggest that an exocyst-SAP102-NMDAR complex is an important component of NMDAR trafficking.
Laboratory of Neurochemistry, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Building 50, Room 4146, 50 South Drive, Bethesda, MD 20892-8027, USA. sansn@nidcd.nih.gov
Nathalie
N
Sans
Kate
K
Prybylowski
Ronald S
RS
Petralia
Kai
K
Chang
Ya-Xian
YX
Wang
Claudia
C
Racca
Stefano
S
Vicini
Robert J
RJ
Wenthold
D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry; D019556:COS Cells; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D005089:Exocytosis / Q000502:physiology*; D008565:Membrane Proteins; D008954:Models, Biological; D008969:Molecular Sequence Data; D009154:Mutation; D009474:Neurons / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D009479:Neuropeptides / Q000378:metabolism; D011485:Protein Binding; D021381:Protein Transport; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*
D002352:Carrier Proteins; C101195:Dlg3 protein, rat; C076326:Exoc4 protein, rat; D008565:Membrane Proteins; D009479:Neuropeptides; D016194:Receptors, N-Methyl-D-Aspartate