Chronic consumption of a moderately low protein diet does not alter hematopoietic processes in young adult mice.
The current studies examined whether hematopoiesis in the bone marrow and T-cell development in the thymus were attenuated in young adult A/J mice fed a moderately low protein diet (MPD, 50 g protein/kg) for 15 wk compared with mice fed a control protein diet (CPD, 180 g protein/kg). Flow cytometric analyses using antibodies against CD31 and Ly-6C as well as CD4 and CD8 were performed to identify stem, mixed progenitor, erythroid, lymphoid, granuloid and monocytic compartments in the bone marrow and four thymocyte subsets, respectively. Chronic restriction of young adult mice to MPD neither decreased the cellularity nor altered the distribution of subpopulations in either primary tissue. Subsequently, a new set of mice were provided with CPD and a low protein diet (LPD, 25 g protein/kg). After 5 wk, body and thymus weights in LPD group were reduced 26 and 30%, respectively, which was accompanied by a 505% increase in serum corticosterone. Surprisingly, LPD did not alter the number or distribution of cells in the bone marrow and the percentages of thymocyte subsets, supporting the findings from the MPD group. We conclude that chronic consumption of a marginal protein diet by young adult mice does not disrupt hematopoietic processes.
Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824, USA.
Zhixin L
ZL
Huang
Pamela J
PJ
Fraker
D000818:Animals; D001835:Body Weight / Q000187:drug effects / Q000502:physiology; D003345:Corticosterone / Q000097:blood; D018753:Diet, Protein-Restricted; D004044:Dietary Proteins / Q000494:pharmacology*; D005260:Female; D006410:Hematopoiesis / Q000187:drug effects / Q000502:physiology*; D008670:Metals / Q000097:blood; D051379:Mice; D008805:Mice, Inbred A; D012709:Serum Albumin / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D014131:Trace Elements / Q000097:blood
D004044:Dietary Proteins; D008670:Metals; D012709:Serum Albumin; D014131:Trace Elements; D003345:Corticosterone