Identification and characterization of cytopathogenic Mycoplasma hyorhinis from swine farms with a history of abortions.
A virus-like cytopathic agent isolated from swine farms with a history of recurrent abortion episodes was investigated. We employed a differential display reverse transcription-polymerase chain reaction (ddRT-PCR) to obtain genetic information of the cytopathic agent. Partial nucleotide sequence (527 bp) obtained from differentially displayed PCR fragments showed 88.7% similarity with the 23S rRNA gene of Mycoplasma hyopneumoniae. Unexpectedly, the 5' portion (1-333 bp) of the sequence shared 96.1% similarity with 5' untranslated region (UTR) of human prostate tumor inducing gene 1 (PTI-1). Cytopathic effects and extranuclear DNA fluorescence were no longer observed when BM-cyclin was added in the culture medium, suggesting that BM-cyclin sensitive mycoplasma-like organisms caused the cell death. Further evidence supporting the cytopathic agent as a mycoplasma-like organism was obtained by the capability of (3)H-thymidine and (3)H-uridine incorporation, a single peak in buoyant density gradient profile (1.20-1.24 g/ml), and ultrastructural morphology. Unlike M. hyopneumoniae, the organism was not propagated in Friis medium. Nucleotide sequence of 16S rRNA obtained from the cytopathic agent showed 0.8-1.0% divergences with other M. hyorhinis strains, suggesting that the newly isolated cytopathogenic swine mycoplasma was a variant form of M. hyorhinis. Striking homology between a portion of the 23S rRNA gene of M. hyorhinis and 5' UTR of human PTI-1 implicated that M. hyorhinis might potentially be related to the evolution of human PTI-1.
Department of Clinical and Population Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul 55108, USA.
Jin Ho
JH
Shin
Han Soo
HS
Joo
Won Hyung
WH
Lee
Ho Bong
HB
Seok
Maria
M
Calsamig
Carlos
C
Pijoan
Thomas W
TW
Molitor
D000034:Abortion, Veterinary / Q000382:microbiology*; D000818:Animals; D001483:Base Sequence; D001854:Bone Marrow Cells / Q000166:cytology / Q000382:microbiology; D002455:Cell Division; D002499:Centrifugation, Density Gradient / Q000662:veterinary; D004269:DNA, Bacterial / Q000737:chemistry; D004224:Diterpenes / Q000494:pharmacology; D005260:Female; D008911:Minocycline / Q000494:pharmacology; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D009175:Mycoplasma Infections / Q000382:microbiology / Q000662:veterinary*; D011247:Pregnancy; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D012008:Recurrence; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D014132:Trachea / Q000382:microbiology
D004269:DNA, Bacterial; D004224:Diterpenes; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; C074450:BM-cyclin; D008911:Minocycline