[Study on the effect of alcohol on embryonic development by using in vitro post-implantation rat whole embryo culture].
In order to explore the effects of drinking alcohol during pregnancy on embryonic development and its mechanisms, a post-implantation whole embryo culture(WEC) technique was used. The 9.5 day rat embryos were explanted in rat serum medium(immediately centrifugal serum, ICS) with alcohol(0.0.4.1.0, 2.00 and 4.00 g/L), and cultured for 48 hours. The index of embryo development and morphological scores induced by alcohol were observed. The result showed that alcohol had obviously effects on the development and growth of embryos with a dose-response relationship. Embryonic development of 0.4 g/L group was not significantly different from the control group, whereas 1.0 g/L group could interfere with the development score of mid-brain, forebrain, neurotube, and visceral yolk sac(VYS) circle obviously. All scores of the 2.00 g/L group were significantly lower than that of control group (P < 0.05). Moreover, the rate of embryo lethality and teratogenecity were obvious increased. It is concluded that alcohol has developmental toxicity and teratogenicity. The target organ affected by alcohol is brain. The effects of alcohol on the developmental differentiation of visceral yolk sac and DNA synthesis are probably related to its developmental abnormalities.
Department of Environmental Health, School of Public Health, Chengdu 610041, China.
W
W
Qu
B
B
Zhang
D
D
Wu
W
W
Wu
D000818:Animals; D046508:Culture Techniques; D004247:DNA / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D000431:Ethanol / Q000633:toxicity*; D005260:Female; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013723:Teratogens / Q000633:toxicity*
D013723:Teratogens; D000431:Ethanol; D004247:DNA