[The biology of aerobic methylobacteria capable of degrading halomethanes].
Recent data on the biology of aerobic methylotrophic bacteria capable of utilizing toxic halogenated methane derivatives as sources of carbon and energy are reviewed, with particular emphasis on the taxonomic, physiological, and biochemical diversity of mono- and dihalomethane-degrading methylobacteria and the enzymatic and genetic aspects of their primary metabolism. The initial steps of chloromethane dehalogenation to formate and HCl through a methylated corrinoid and methyletrahydrofolate are catalyzed by inducible cobalamin methyl transferase, made up of two proteins (CmuA and CmuB) encoded by the cmuA and cmuB genes. At the same time, the primary dehalogenation of dichloromethane to formaldehyde and HCl is catalyzed by cytosolic glutathione transferase with S-chloromethylglutathione as an intermediate. The latter enzyme is encoded by the structural dcmA gene and is under the negative control of the regulatory dcmR gene. In spite of considerable progress in the study of halomethane dehalogenation, some aspects concerning the structural and functional organization of this process and its regulation remain unknown, including the mechanisms of halomethane transport, the release of toxic dehalogenation products (S-chloromethylglutathione, CH2O, and HCl) from cells, and the maintenance of intracellular pH. Of particular interest is quantitative evaluation of the ecophysiological role of aerobic methylobacteria in the mineralization of halomethanes and protection of the biosphere from these toxic pollutants.
Trotsenko
Iu A
IuA
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, Pushchino, Moscow Oblast, 142290 Russia. trotsenko@ibpm.serpukhov.su
Doronina
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NV
D005557:Formaldehyde / Q000378:metabolism; D005561:Formates / Q000378:metabolism; D005798:Genes, Bacterial; D005978:Glutathione / Q000031:analogs & derivatives* / Q000096:biosynthesis; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism*; D006851:Hydrochloric Acid / Q000378:metabolism; D008697:Methane / Q000031:analogs & derivatives / Q000378:metabolism*; D008737:Methyl Chloride / Q000378:metabolism; D008752:Methylene Chloride / Q000378:metabolism; D020580:Methylobacterium / Q000145:classification / Q000502:physiology*; D008780:Methyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018085:Regulon
D005561:Formates; C030544:formic acid; C085664:S-chloromethylglutathione; D005557:Formaldehyde; D008752:Methylene Chloride; D008737:Methyl Chloride; D008780:Methyltransferases; D005982:Glutathione Transferase; D005978:Glutathione; D008697:Methane; D006851:Hydrochloric Acid