Distribution of glucokinase, glucose transporter GLUT2, sulfonylurea receptor-1, glucagon-like peptide-1 receptor and neuropeptide Y messenger RNAs in rat brain by quantitative real time RT-PCR.
Glucokinase (GK), glucose transporter GLUT2, sulfonylurea receptor-1 (SUR1), glucagon-like peptide-1 receptor (GLP-1R) and neuropeptide Y (NPY) have been proposed to be involved in central glucose sensing or regulation of food intake. In this study, we combined tissue micropunch and real time reverse transcription polymerase chain reaction (RT-PCR), and measured GK, GLUT2, SUR1, GLP-1R and NPY mRNA expression in discrete areas in the hypothalamus and the hindbrain.
Pennington Biomedical Research Center C1038, Louisiana State University, 6400 Perkins Rd, Baton Rouge 70808, USA.
Bing
B
Li
Xiaochun
X
Xi
David S
DS
Roane
Donna H
DH
Ryan
Roy J
RJ
Martin
D018528:ATP-Binding Cassette Transporters; D000818:Animals; D001069:Appetite Regulation / Q000235:genetics*; D001921:Brain / Q000378:metabolism*; D000067757:Glucagon-Like Peptide-1 Receptor; D005941:Glucokinase / Q000235:genetics*; D005947:Glucose / Q000378:metabolism; D051273:Glucose Transporter Type 2; D007031:Hypothalamus / Q000378:metabolism; D008297:Male; D009004:Monosaccharide Transport Proteins / Q000235:genetics*; D009478:Neuropeptide Y / Q000235:genetics*; D015221:Potassium Channels / Q000235:genetics*; D024661:Potassium Channels, Inwardly Rectifying; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011955:Receptors, Drug / Q000235:genetics*; D018027:Receptors, Glucagon / Q000235:genetics*; D012249:Rhombencephalon / Q000378:metabolism; D064233:Sulfonylurea Receptors
D018528:ATP-Binding Cassette Transporters; C577509:Abcc8 protein, rat; C000593835:Glp1r protein, rat; D000067757:Glucagon-Like Peptide-1 Receptor; D051273:Glucose Transporter Type 2; D009004:Monosaccharide Transport Proteins; D009478:Neuropeptide Y; D015221:Potassium Channels; D024661:Potassium Channels, Inwardly Rectifying; D012333:RNA, Messenger; D011955:Receptors, Drug; D018027:Receptors, Glucagon; D064233:Sulfonylurea Receptors; D005941:Glucokinase; D005947:Glucose