Preliminary characterization of a light-rare-earth-element-binding peptide of a natural perennial fern Dicranopteris dichotoma.
A light-rare-earth-element (LREE)-binding peptide was isolated from LREE hyperaccumulator Dicranopteris dichotomaleaves and characterized in terms of molecular weight and ultraviolet absorption spectrum. The molecular weight of the LREE-binding peptide was determined to be 2208 Da by matrix-assisted laser-desorption ionization-time of flight mass spectrometry (MALDI-TOFMS). The characteristic ultraviolet absorption spectrum of the peptide was observed at 220-300 nm, suggesting that the peptide chain contained aromatic amino acids. Compared to the unique features of the phytochelatins with a low absorption at 280 nm, the LREE-binding peptide is unlikely to be a typical phytochelatin. The present study suggests that the LREE-binding peptide is probably a natural peptide in D. dichotoma, and it may play an important role in hyperaccumulation of LREEs.
Wang
Haiou
H
Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, 100085, Beijing, China.
Shan
Xiao-Quan
XQ
Zhang
Shuzhen
S
Wen
Bei
B
D002563:Cerium / Q000378:metabolism; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D029624:Ferns / Q000737:chemistry*; D007811:Lanthanum / Q000378:metabolism; D008674:Metals, Rare Earth / Q000378:metabolism*; D008860:Microscopy, Ultraviolet; D008970:Molecular Weight; D009354:Neodymium / Q000378:metabolism; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D011221:Praseodymium / Q000378:metabolism; D011485:Protein Binding; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D008674:Metals, Rare Earth; D010455:Peptides; D010940:Plant Proteins; D009354:Neodymium; D002563:Cerium; D007811:Lanthanum; D011221:Praseodymium