The application potential of sintered beta-dicalcium pyrophosphate in total joint arthroplasty.
An in vitro bone cell culture model was used to evaluate the potential application of sintered beta-dicalcium pyrophosphate (SDCP) in arthroplasty surgery. Primary osteoclasts and osteoblasts were cocultured with different sizes of SDCP particles. The changes in cell counts and the synthesis and secretion of alkaline phosphatase, acid phosphatase, and prostaglandin E(2) in response to the SDCP particles were monitored. When bone cells were cultured with SDCP particles smaller than 53 microm, both the osteoblast and osteoclast cell counts decreased significantly. When the SDCP particles were larger than 177 microm, although the osteoblast population increased significantly, the osteoclast population decreased significantly. Simultaneously, the titer of prostaglandin E(2) in the medium and the cytoplasmic prostaglandin E(2) increased significantly. We concluded that SDCP is a potentially useful bioceramic for the prevention of osteoclast-mediated periprosthetic osteolysis.
Department of Biomedical Engineering, National Taiwan University Hospital, No. 1 Jen-Ai Road, Taipei, Taiwan, ROC.
Jui-Sheng
JS
Sun
Yang-Hwei
YH
Tsuang
Feng-Huei
FH
Lin
Li-Ting
LT
Chen
Yi-Shiong
YS
Hang
Hwa-Chang
HC
Liu
D020341:Absorbable Implants; D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D019643:Arthroplasty, Replacement; D001672:Biocompatible Materials / Q000494:pharmacology*; D018786:Bone Substitutes / Q000494:pharmacology*; D002131:Calcium Pyrophosphate / Q000494:pharmacology*; D002452:Cell Count; D002455:Cell Division; D002478:Cells, Cultured; D018920:Coculture Techniques; D015232:Dinoprostone / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D010010:Osteoclasts / Q000166:cytology / Q000378:metabolism*; D010316:Particle Size; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000166:cytology
D001672:Biocompatible Materials; D018786:Bone Substitutes; C476842:sintered beta-dicalcium pyrophosphate; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D015232:Dinoprostone; D002131:Calcium Pyrophosphate