Influence of nonsteroidal anti-inflammatory drug treatment duration and time of onset on recovery from exercise-induced muscle damage in rats.
OBJECTIVE
To determine if duration and time of onset of treatment with diclofenac sodium influence force recovery after exercise-induced muscle damage in rats.


METHODS
Randomized placebo-controlled trial.


METHODS
Animal laboratory.


METHODS
A total of 217 female adult Wistar rats.


METHODS
Rats were submitted to a protocol consisting of 450 eccentric contractions of the ankle dorsiflexors. Treatment by gavage with diclofenac sodium (1 mg/kg, twice daily) was started at different times pre- and postprotocol or for various treatment durations.


METHODS
In vitro contractile properties.


RESULTS
When treatment was initiated shortly postprotocol, force recovery was roughly proportional to treatment duration during the first 3 days but not at 7 and 28 days postprotocol. A 7-day treatment was no more effective than 1- or 2-day treatments when force was measured at 7 and 28 days; however, such prolonged treatment had no deleterious effect on muscle force at either time. A single-dose prophylactic treatment was as effective as a 2-day treatment initiated soon after the protocol when force was assessed 2 days postprotocol; on the other end, a treatment delayed for 3 days had no effect when force was measured at 7 days.


CONCLUSIONS
Treatment with diclofenac sodium extending past the acute inflammatory phase was no more effective than short and timely treatment in this model of skeletal muscle damage.
CHUL Research Center, Laval University, Quebec City, QC, Canada.
Benoît M
BM
Lapointe
Pierre
P
Frémont
Claude H
CH
Côté
D000208:Acute Disease; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage* / Q000494:pharmacology; D004008:Diclofenac / Q000008:administration & dosage* / Q000494:pharmacology; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D005260:Female; D007249:Inflammation; D007537:Isometric Contraction / Q000187:drug effects; D009220:Myositis / Q000188:drug therapy* / Q000209:etiology / Q000503:physiopathology / Q000517:prevention & control*; D010805:Physical Conditioning, Animal / Q000009:adverse effects*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function / Q000187:drug effects*; D013997:Time Factors; D016896:Treatment Outcome
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004008:Diclofenac