Gene delivery to the lung by means of syngeneic fibroblasts: an experimental model.
An experimental model, in which exogenous gene expression in the lung is achieved, has been established. A fibroblast cell line was transfected with the lacZ gene and was administered to syngeneic mice by either intravenous or intratracheal injection. Enzyme-linked immunosorbent assay and histochemical detection of beta-galactosidase revealed efficient gene delivery to the lung by either route. Kinetic studies demonstrated that the expression peaked immediately after the injection, and this high level was maintained for 7 days by intratracheal and for 14 days by intravenous administration. This system may have potential relevance for certain experimental models requiring specific gene delivery to the lung.
Department of Respirology (B2), Graduate School of Medicine, Chiba University, Japan.
Akira
A
Suda
Yuichi
Y
Takiguchi
Shigenari
S
Omori
Hiroshi
H
Miyazawa
Takaaki
T
Sugimoto
Katsushi
K
Kurosu
Koichiro
K
Tatsumi
Nobuhiro
N
Tanabe
Takayuki
T
Kuriyama
Kenzo
K
Hiroshima
Hiroshi
H
Kimura
D000818:Animals; D017690:Cell Transplantation; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000201:enzymology* / Q000637:transplantation; D005696:Galactosidases / Q000235:genetics* / Q000378:metabolism; D018014:Gene Transfer Techniques; D022761:Gene Transfer, Horizontal; D005822:Genetic Vectors; D007763:Lac Operon / Q000235:genetics; D008168:Lung / Q000166:cytology / Q000201:enzymology*; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D013047:Specific Pathogen-Free Organisms; D014162:Transfection
D005696:Galactosidases