[Zinc protective effects on pig retinal pigment epithelial cell damage of lipid peroxide induced by 2450 MHz microwave].
To observe the lipid peroxide damage in the cultured pig retinal pigment epithelial cell(RPE) induced by microwave and the protective effects by zinc (Zn). RPE cells were divided into 3 groups in terms of microwave intensity(the group of 10, 20 and 30 mW.cm-2) and each group was exposed to microwave for 1 hour. After adding ZnSO4 into DMEM culture fluid, the group of 30 mW.cm-2 was exposed to microwave. The activity of SOD and the content of MDA were measured. The results showed that the activity of SOD decreased significantly compared with the control group(P < 0.001). The content of MDA increased significantly compared with the control group(P < 0.001). Administration of Zn alleviated the increase of MDA and decrease of SOD. In the experiment, 2450 MHz microwave induces the lipid peroxide damage in RPE cells. Zn can enhance the antioxidation ability of cells and alleviate the damage to some extent.
Faculty of Military Health Service and Statistics, Fourth Military Medical University, Xi'an 710033, China.
Z
Z
Deng
X
X
Liu
J
J
Chen
R
R
Yang
D000818:Animals; D002478:Cells, Cultured; D008054:Lipid Peroxides / Q000378:metabolism*; D008315:Malondialdehyde / Q000378:metabolism; D008872:Microwaves / Q000009:adverse effects*; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D013482:Superoxide Dismutase / Q000378:metabolism; D013552:Swine; D019287:Zinc Sulfate / Q000494:pharmacology*
D008054:Lipid Peroxides; D008315:Malondialdehyde; D019287:Zinc Sulfate; D013482:Superoxide Dismutase