Novel human G-protein-coupled receptors.
G-protein-coupled receptors (GPCRs) are important mediators of signal transduction and targets for pharmacological therapeutics. Novel receptor-ligand systems have been discovered through the identification and analysis of orphan GPCRs (oGPCRs). Here we describe the discovery of seven novel human genes encoding oGPCRs. Each novel oGPCR gene was discovered using customized searches of the GenBank genomic databases with previously known GPCR-encoding sequences. The expressed genes can now be used in assays to determine endogenous and pharmacological ligands. GPR133, GPR134, GPR135, GPR136, and GPR137 share identities with a prostate-specific odorant-like GPCR-encoding gene (PSGR). GPR138 and GPR139 share identities with an odorant-like gene derived from human erythroid cells. Transcripts encoding GPR133, GPR134, GPR135, GPR136, and GPR137 were detected in various CNS tissues. The expression of odorant-like genes in non-olfactory tissues requires further clarification, which may be achieved through the search for endogenous cognate ligands for these and other oGPCRs.
Department of Pharmacology, Medical Sciences Building, Room 4352, University of Toronto, 1 King's College Circle, Toronto, Ont, Canada M5S 1A8.
William B
WB
Vanti
Tuan
T
Nguyen
Regina
R
Cheng
Kevin R
KR
Lynch
Susan R
SR
George
Brian F
BF
O'Dowd
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D020962:Heterotrimeric GTP-Binding Proteins / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis; D018035:Receptors, Odorant / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D014018:Tissue Distribution
D012333:RNA, Messenger; D018035:Receptors, Odorant; D020962:Heterotrimeric GTP-Binding Proteins