Alloantibodies that react with subsets of human T cells.
Using the two color fluorescence (TCF) method, alloantibodies against subsets of T cells could be detected in sera from pregnant women with strong HLA antibodies. To preclude interference of these HLA antibodies with the recognition of the T cell antibodies, serum donors were selected which were HLA-Al, -B8, -DRw3. Their sera were tested on a panel of individuals homozygous for HLA-Al, -B8, -DRw3. By enriching peripheral mononuclear blood lymphocytes for Tgamma cells it could be shown that some of the sera reacted mainly with Tgamma and others with Tmu lymphocytes, while some sera reacted with both.
Department of Immunology, London Hospital Medical College, London, UK.
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van Leeuwen
D000328:Adult; D000818:Animals; D000961:Antilymphocyte Serum / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic; D004912:Erythrocytes; D005260:Female; D006680:HLA Antigens / Q000276:immunology; D015788:HLA-A1 Antigen / Q000032:analysis; D015795:HLA-B8 Antigen / Q000032:analysis; D006684:HLA-DR Antigens / Q000032:analysis; D015803:HLA-DR3 Antigen; D006648:Histocompatibility; D006720:Homozygote; D006801:Humans; D007114:Immunization; D007518:Isoantibodies / Q000276:immunology*; D011247:Pregnancy; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology; D012397:Rosette Formation; D012756:Sheep; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D000961:Antilymphocyte Serum; D006680:HLA Antigens; D015788:HLA-A1 Antigen; D015795:HLA-B8 Antigen; D006684:HLA-DR Antigens; D015803:HLA-DR3 Antigen; D007518:Isoantibodies; D016692:Receptors, Antigen, T-Cell, gamma-delta