Variations in lectin-binding on the zona pellucida during oocyte growth in some wild ungulates.
The aim of the present study was to examine the glycoconjugate modifications occurring in the zona pellucida during oocyte growth in fallow, red and roe deer using a battery of lectins combined with sialidase digestion and chemical treatments. This histochemical approach allowed us to sequence the oligosaccharidic side chains of the zona pellucida glycoproteins in these wild ungulates. The most effective lectins in the zona pellucida of these species were SBA, PNA, RCA-I GSA-IB4, and WGA, indicating the presence of beta-D-N-Acetylgalactosamine, beta-D-Galactose, alpha-D-Galactose and N-Acetylglucosamine residues. Additionally, sialic acid moieties were demonstrated. We also observed differences in the glycosidic residue content and in their spatial distribution, depending on the species and stage of follicle development.
Parillo
F
F
Department of Biopathological Veterinary Science, Faculty of Veterinary Medicine, Universita degli Studi di Perugia, via San Costanzo 4, 06126 Perugia, Italy. anatvet@unipg.it
Diverio
S
S
Romeo
G
G
Fagioli
O
O
D000818:Animals; D000835:Animals, Wild; D001665:Binding Sites; D003670:Deer; D004187:Disaccharides / Q000494:pharmacology; D005260:Female; D037102:Lectins; D009005:Monosaccharides / Q000494:pharmacology; D009439:Neuraminidase; D009865:Oocytes / Q000166:cytology*; D015044:Zona Pellucida / Q000648:ultrastructure*
D004187:Disaccharides; D037102:Lectins; D009005:Monosaccharides; D009439:Neuraminidase