Effect of hyperosmolarity on recombinant protein productivity in baculovirus expression system.
A lot of strategies were applied to improve recombinant protein productivity in the baculovirus expression system. In this study we propose for foreign protein production fed-batch cultivation method at hyperosmotic environment induced by increased NaCl content. Obtained results suggested relatively high tolerance and adaptation abilities of Tn-5 insect cells exposed to hyperosmotic stress. The cells under hyperosmotic conditions increased the specific rate of glucose consumption and lactate production. The release of additional energy and precursors as a result of increased metabolism by osmotically stressed culture was involved in recombinant protein synthesis. Recombinant nucleoprotein productivity in nutritional feeding cultures exposed to hyperosmolarity was about 72% higher than that obtained in batch culture at physiological osmolarity, but 31% was a result of feeding and the rest 41% was a result of hyperosmolarity and increasing Na(+) concentration.
Olejnik
A
A
Department of Biotechnology and Food Microbiology, Agricultural University in Poznan, Wojska Polskiego 48 Street, Poznan, Poland. aolejnik@owl.au.poznan.pl
Grajek
W
W
Marecik
R
R
D000818:Animals; D019149:Bioreactors; D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D005947:Glucose / Q000378:metabolism*; D019344:Lactic Acid / Q000096:biosynthesis*; D009036:Moths / Q000254:growth & development* / Q000378:metabolism* / Q000821:virology; D017924:Nucleopolyhedroviruses / Q000254:growth & development* / Q000378:metabolism* / Q000648:ultrastructure; D009698:Nucleoproteins / Q000096:biosynthesis* / Q000235:genetics; D009994:Osmolar Concentration; D009997:Osmotic Pressure; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
D009698:Nucleoproteins; D011994:Recombinant Proteins; D019344:Lactic Acid; D005947:Glucose