cDNA microarray analysis reveals chop-10 plays a key role in Sertoli cell injury induced by bisphenol A.
We examined the time course of changes in gene expression in detail using cDNA microarray analysis of mouse Sertoli TTE3 cells treated with bisphenol A (BPA). A subtoxic dose of BPA (200 microM) transiently increased intracellular Ca(2+) concentration and time-dependently induced an increase in mRNA level of 78-kDa glucose-regulated protein, indicating that BPA induces endoplasmic reticulum stress. Of the 865 genes analyzed, 31 genes showed increased levels of expression. TaqMan analysis confirmed that the mRNA levels of chop-10, fra-2, c-myc, and ornithine decarboxylase were increased, and showed that chop-10 is the most sensitive gene. The expression level of chop-10 protein and cell injury induced by BPA were significantly reduced in stable TTE3 cells overexpressing full-length chop-10 antisense RNA. We conclude that chop-10 plays a key role in Sertoli cell injury induced by BPA.
Division of Molecular Genetics, Life Science Research Center, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama City, Toyama 930-0194, Japan. ytabu@ms.toyama-mpu.ac.jp
Yoshiaki
Y
Tabuchi
Takashi
T
Kondo
D000818:Animals; D001559:Benzhydryl Compounds; D022762:CCAAT-Enhancer-Binding Proteins / Q000502:physiology*; D002460:Cell Line; D004721:Endoplasmic Reticulum / Q000187:drug effects; D004968:Estrogens, Non-Steroidal / Q000633:toxicity*; D020869:Gene Expression Profiling; D007700:Kinetics; D008297:Male; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D010636:Phenols / Q000633:toxicity*; D012333:RNA, Messenger / Q000096:biosynthesis; D012708:Sertoli Cells / Q000187:drug effects* / Q000378:metabolism; D051742:Transcription Factor CHOP; D014157:Transcription Factors / Q000502:physiology*; D015854:Up-Regulation; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D022762:CCAAT-Enhancer-Binding Proteins; D004968:Estrogens, Non-Steroidal; D010636:Phenols; D012333:RNA, Messenger; D051742:Transcription Factor CHOP; D014157:Transcription Factors; C497250:Ddit3 protein, mouse; C006780:bisphenol A; D000098826:Bisphenol A Compounds