Lipid metabolism in the embryos of diabetic rats during early organogenesis: modulatory effect of prostaglandin E2.
The purpose of this work was to evaluate de novo lipid biosynthesis and the lipid profile, and to study the effect of prostaglandin E2 (PGE2; prostaglandin has previously been found to be involved in diabetes embryopathy) on lipid metabolism in embryos from control and streptozotocin-induced diabetic rats during organogenesis. Increased levels of triacylglycerols were found in embryos of diabetic rats compared with controls, whereas no differences were detected in the levels of cholesterol, cholesterylester, phosphatidylcholine and phosphatidylethanolamine. When the de novo synthesis of lipids in the embryo was studied using [14C]acetate as a tracer, a diminished rate of incorporation of [14C]acetate into the evaluated lipid classes was detected in the diabetic embryo compared with controls. Addition of PGE2 did not modify the incorporation of [14C]acetate into any of the lipid species of control embryos, but enhanced the incorporation of [14C]acetate into triacylglycerol, cholesterylesters, phosphatidylcholine and phosphatidylethanolamine of embryos from diabetic rats. The study's results show alterations in both synthesis and concentrations of lipids in the embryos of diabetic rats. Interestingly, the results demonstrate that the addition of PGE2, a prostaglandin that reverses the embryonic morphological abnormalities induced by diabetes, prevents disturbances in embryo lipid synthesis caused by diabetes.
Centro de Estudios Farmacológicos y Botánicos (CEFYBO), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Serrano 669, (1414) Buenos Aires, Argentina.
Debora
D
Sinner
J Matías
JM
Caviglia
Alicia
A
Jawerbaum
R Ariel
RA
Igal
Elida
E
Gonzalez
D019342:Acetic Acid / Q000378:metabolism; D000818:Animals; D002250:Carbon Radioisotopes; D002784:Cholesterol / Q000032:analysis / Q000378:metabolism; D002788:Cholesterol Esters / Q000032:analysis / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D015232:Dinoprostone / Q000494:pharmacology*; D004622:Embryo, Mammalian / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D050356:Lipid Metabolism; D008055:Lipids / Q000096:biosynthesis; D038081:Organogenesis; D010713:Phosphatidylcholines / Q000032:analysis / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000032:analysis / Q000378:metabolism; D011247:Pregnancy; D011254:Pregnancy in Diabetics / Q000378:metabolism*; D051381:Rats; D014280:Triglycerides / Q000032:analysis / Q000378:metabolism
D002250:Carbon Radioisotopes; D002788:Cholesterol Esters; D008055:Lipids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D014280:Triglycerides; D002784:Cholesterol; D015232:Dinoprostone; D019342:Acetic Acid