Technical note: Use of RFLP to characterize Lactococcus lactis strains producing exopolysaccharides.
Restriction fragment length polymorphism (RFLP) is used to differentiate microorganisms by analysis of their DNA restriction patterns. A modified RFLP procedure is proposed for the rapid characterization of Lactococcus lactis strains producing exopolysaccharides (EPS). The availability of such effective cataloging system is likely to benefit research aimed at identifying lactococcal strains that produce novel EPS.
Département de Biochimie et de Microbiologie, Faculté des Sciences et de Génie, Université Laval, Québec, Canada, G1K 7P4.
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D004269:DNA, Bacterial / Q000032:analysis / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D013294:Lactococcus lactis / Q000145:classification* / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D011134:Polysaccharides / Q000096:biosynthesis* / Q000235:genetics
D004269:DNA, Bacterial; D011134:Polysaccharides; C024408:endodeoxyribonuclease SacI; D015252:Deoxyribonucleases, Type II Site-Specific; C052694:GRCGYC-specific type II deoxyribonucleases; C024400:GTYRAC-specific type II deoxyribonucleases