Calotropis procera (Sodom apple)--a potential material for enzyme purification.
A simple method based on precipitation with Calotropis procera latex was developed for the purification of crude enzyme from fermentation broth. C. procera latex (10(-2) dilution) clarified and concentrated the crude amylase of Aspergillus oryzae 4-fold with 97% recovery of the initial amylase activity in the filtrate in a single step operation. The latex was stable at pH < or = 4.5 and there was no significant difference (P < or = 0.05) in the purification potential of the latex at 4 and 28 degrees C. This method could prove useful to developing economies of the tropics where ambient temperature is around 28 degrees C.
Kareem
S O
SO
Department of Microbiology, University of Agriculture, P.M.B 2240, Abeokuta, Nigeria.
Akpan
I
I
Osho
M B
MB
D000681:Amylases / Q000302:isolation & purification*; D001230:Aspergillus / Q000201:enzymology; D031107:Calotropis / Q000737:chemistry*; D011232:Chemical Precipitation; D005285:Fermentation; D005374:Filtration; D006863:Hydrogen-Ion Concentration; D007840:Latex / Q000737:chemistry*
D007840:Latex; D000681:Amylases