Evaluation of a new serotyping assay for detection of anti-hepatitis C virus type-specific antibodies in serum samples.
The performance of a new version (HC03) of the hepatitis C virus (HCV) serotyping 1-6 assay (Abbott Murex Laboratories), a specific test for serological determination of HCV types, was evaluated using a selected panel of 180 HCV RNA-positive sera. HC03 was more sensitive than the current HC02 version, typing 53 (37.6%) of 141 samples which were not typable with HC02. Furthermore, the HC03 specificity was 94.1% as evaluated with a panel of 22 genotyped samples. This new version of the test improves the quality of the serological approach to HCV type determination.
Gault
Elyanne
E
Laboratoire de Bactériologie-Virologie-Hygiène, Hôpital Avicenne, Assistance Publique-Hôpitaux de Paris, EA3406 ATHSCO, UFR Santé, Médecine, Biologie Humaine, Université Paris 13, 93009 Bobigny cedex, France. elyanne.gault@avc.ap-hop-paris.fr
Soussan
Patrick
P
Morice
Yoann
Y
Sanders
Lara
L
Berrada
Assia
A
Rogers
Brian
B
Dený
Paul
P
D005814:Genes, Viral; D005838:Genotype; D016174:Hepacivirus / Q000145:classification* / Q000235:genetics / Q000276:immunology; D006526:Hepatitis C / Q000276:immunology / Q000821:virology; D018937:Hepatitis C Antibodies / Q000097:blood*; D006801:Humans; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012680:Sensitivity and Specificity; D012703:Serotyping / Q000379:methods* / Q000706:statistics & numerical data; D017361:Viral Nonstructural Proteins / Q000235:genetics; D012324:RNA-Dependent RNA Polymerase
D018937:Hepatitis C Antibodies; D017361:Viral Nonstructural Proteins; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase