High-performance liquid chromatography with diamond ATR-FTIR detection for the determination of carbohydrates, alcohols and organic acids in red wine.
A horizontal diamond attenuated total reflection (ATR) element has been incorporated in a flow-through cell with low dead volume and used for on-line mid-IR detection in high-performance liquid chromatography. The chemical inertness of the ATR element permitted the use of a strongly acidic mobile phase in the isocratic separation. The hyphenation was used for the analysis of organic acids, sugars and alcohols in red wine. In the case of co-eluting analytes multivariate curve resolution-alternating least squares (MCR-ALS) was successfully employed for quantitative analysis.
Edelmann
Andrea
A
Institute of Chemical Technologies and Analytics, Vienna University of Technology, Getreidemarkt 9/164-AC, Austria.
Diewok
Josef
J
Baena
Josefa Rodriguez
JR
Lendl
Bernhard
B
D000438:Alcohols / Q000032:analysis*; D002241:Carbohydrates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D003627:Data Interpretation, Statistical; D018130:Diamond; D009930:Organic Chemicals / Q000032:analysis*; D017550:Spectroscopy, Fourier Transform Infrared; D014920:Wine / Q000032:analysis*
D000438:Alcohols; D002241:Carbohydrates; D009930:Organic Chemicals; D018130:Diamond