Biodegradation of chloronaphthalenes and polycyclic aromatic hydrocarbons by the white-rot fungus Phlebia lindtneri.
The biodegradation of chloronaphthalene (CN) and polycyclic aromatic hydrocarbons by the white-rot fungus Phlebia lindtneri, which can degrade dichlorinated dioxins and non-chlorinated dioxin-like compounds, was investigated. Naphthalene, phenanthrene, 1-chloronaphthalene (1-CN) and 2-chloronaphthalene (2-CN) were metabolized by the fungus to form several oxidized products. Naphthalene and phenanthrene were metabolized to the corresponding hydroxylated and dihydrodihydroxylated metabolites. 2-CN was metabolized to 3-chloro-2-naphtol, 6-chloro-1-naphtol and two other chloronaphtols, CN-dihydrodiols and CN-diols. Significant inhibition of the degradation of these substrates was observed when they were incubated with the cytochrome P-450 monooxygenase inhibitors 1-aminobenzotriazole and piperonyl butoxide. These results suggest that P. lindtneri initially oxidizes these substrates by a cytochrome P-450 monooxygenase.
Laboratory of Systematic Forest and Forest Products Sciences, Department of Forest and Forest Products Sciences, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581 Fukuoka, Japan.
T
T
Mori
S
S
Kitano
R
R
Kondo
D001673:Biodegradation, Environmental; D002851:Chromatography, High Pressure Liquid; D065607:Cytochrome P-450 Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004147:Dioxins / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006900:Hydroxylation; D009281:Naphthalenes / Q000378:metabolism*; D010084:Oxidation-Reduction; D010616:Phenanthrenes / Q000378:metabolism; D010882:Piperonyl Butoxide / Q000494:pharmacology; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*; D020072:Polyporales / Q000201:enzymology / Q000378:metabolism*; D014230:Triazoles / Q000494:pharmacology
D065607:Cytochrome P-450 Enzyme Inhibitors; D004147:Dioxins; D004791:Enzyme Inhibitors; D009281:Naphthalenes; D010616:Phenanthrenes; D011084:Polycyclic Aromatic Hydrocarbons; D014230:Triazoles; C033020:1-aminobenzotriazole; C031181:phenanthrene; C409928:2-chloronaphthalene; D003577:Cytochrome P-450 Enzyme System; C031337:1-chloronaphthalene; D010882:Piperonyl Butoxide; C020887:dibenzo(1,4)dioxin