Demonstration of species-specific and cross-reactive components of hydatid cyst fluid antigens.
ELISA-hydatid cyst fluid antigen (HCF-Ag-ELISA) and conventional echinococcosis IHAT were assessed for cystic hydatid disease (CHD), confirmed by HCF-Ag immunoblotting assay. The sensitivity of the tests was 94.4% and 83.3% for HCF-Ag-ELISA and IHA respectively. HCF-Ag-ELISA showed some cross-reaction in tumor cases (5.5%), but no cross-reaction was observed in Rheumatoid arthritis patients. IHA showed 100% specificity. To confirm this data SDS-PAGE immunoblotting using HCF-Ag was applied. Echinococcus specific reactions were reported with 44Kda, 34Kda, 29Kda and 8Kda HCF-Ag resolved bands. Cross-reaction was found with 27Kda, 21Kda, 16Kda and 13Kda bands in tumor patients. Moreover, cross-reaction was found with 200Kda, 175Kda, 62Kda, 52Kda and 40Kda in serological anti-Schistosoma antibodies positive cases. So, using specific HCF-Ag components, immunoblotting provided 100% sensitivity and specificity in CHD.
Department of Parasitology, Faculty of Medicine, Benha Branch of Zagazig University, Benha, Egypt.
Samia M
SM
Rashed
Ibrahim A
IA
el-Hyawan
Ibrahim M
IM
Nagaty
Atef H
AH
Hussein
Azza
A
Saad
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology*; D001172:Arthritis, Rheumatoid / Q000276:immunology; D003429:Cross Reactions / Q000276:immunology*; D020420:Cyst Fluid / Q000276:immunology*; D004443:Echinococcosis / Q000097:blood / Q000175:diagnosis* / Q000276:immunology*; D004446:Echinococcus / Q000145:classification / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D009369:Neoplasms / Q000276:immunology; D012547:Schistosoma / Q000276:immunology; D012680:Sensitivity and Specificity; D013045:Species Specificity
D000909:Antibodies, Helminth; D000947:Antigens, Helminth