The inhibitory effect of BSP-A1/-A2 on protein kinase C and tyrosine protein kinase.
Bovine seminal plasma contains a group of similar proteins, namely BSP-A1, BSP-A2, BSP-A3, and BSP-30-kDa (collectively called BSP proteins), and they are secreted by the seminal vesicles. In our study, we purified the BSP-A1/-A2 through affinity chromatography and found for the first time that BSP-A1/-A2 can inhibit the activity of protein kinase C (PKC) and tyrosine protein kinase (TPK). The inhibition was dose dependent. When the PKC and TPK activities are expressed as the logarithm of percentage activity taking the activity in the absence of the BSP-A1/-A2 as 100%, there is a linear relationship between the their activities and the dose of BSP-A1/-A2.
Yu
Bingzhi
B
Department of Biochemistry, China Medical University, 92, North Second Road, Shenyang, Liaoning Province, 110001, People's Republic of China. ybingzhi@yeah.net
Zhao
Yongmei
Y
Zhao
Wei
W
Chen
Fei
F
Liu
Ying
Y
Zhang
Jie
J
Fu
Wei
W
Zong
Zhihong
Z
Yu
Aiming
A
Guan
Yi
Y
D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D004305:Dose-Response Relationship, Drug; D007700:Kinetics; D008297:Male; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors*; D029607:Seminal Vesicle Secretory Proteins / Q000302:isolation & purification / Q000378:metabolism*
D029607:Seminal Vesicle Secretory Proteins; C424303:seminal vesicle secretory protein 109, Bos taurus; D011505:Protein-Tyrosine Kinases; D011493:Protein Kinase C