Protein nanostructures formed via direct-write dip-pen nanolithography.
We report a tip modification strategy coupled with dip-pen nanolithography for depositing proteins on a surface. This methodology offers patterning capabilities on the scale of 45 nm to many micrometers length. The biorecognition properties of nanofeatures composed of immunoglobulin-gamma (IgG) were confirmed by reacting the array with gold nanoparticles coated with anti-IgG. Furthermore, a two component nanoarray was fabricated in direct-write fashion by DPN, and the biorecognition properties of this structure were investigated by AFM without biodiagnostic labels.
Lee
Ki-Bum
KB
Department of Chemistry and Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208, USA.
Lim
Jung-Hyurk
JH
Mirkin
Chad A
CA
D004247:DNA / Q000032:analysis / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D011506:Proteins / Q000032:analysis* / Q000737:chemistry
D011506:Proteins; D004247:DNA