Bleomycin-mediated electrochemotherapy in mouse NR-S1 carcinoma.
OBJECTIVE
To determine whether or not low-voltage electrochemotherapy has cell killing effects.


METHODS
Dorsally transplanted NR-S1 carcinomas in mice were stimulated with electric pulses (40 V/cm) after bleomycin (1 microg/g) had been injected around them. The tumors were fixed with a forceps electrode and electroporation was carried out three times a day for 4 days per week for 2 weeks.


RESULTS
After 8 weeks of experimentation, the tumor had disappeared in four of the ten mice. The cell killing effects were mainly apoptosis and necrosis.


CONCLUSIONS
Electroporation should be clinically introduced into the cosmetic and functional treatment of the head and neck region. Further investigation is also necessary to determine suitable carcinostatic agents and clarify the electric pulse conditions.
Second Department of Oral and Maxillofacial Surgery, Nagasaki University School of Dentistry, 1-7-1 Sakamoto, 852-8588 Nagasaki, Japan. a-kit@net.nagasaki-u.ac.jp
Akira
A
Kitamura
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000627:therapeutic use*; D001761:Bleomycin / Q000627:therapeutic use*; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000628:therapy*; D003131:Combined Modality Therapy; D004566:Electrodes; D018274:Electroporation; D007267:Injections; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D012878:Skin Neoplasms / Q000473:pathology / Q000628:therapy*
D000903:Antibiotics, Antineoplastic; D001761:Bleomycin