Electroenzymatic oxidation of veratryl alcohol by lignin peroxidase.
This paper reports the formation of veratraldehyde by electroenzymatic oxidation of veratryl alcohol (3,4-dimethoxybenzyl alcohol) hybridizing both electrochemical and enzymatic reactions and using lignin peroxidase. The novel electroenzymatic method was found to be effective for replacement of hydrogen peroxide by an electrochemical reactor, which is essential for enzyme activity of lignin peroxidase. The effects of operating parameters such as enzyme dosage, pH, and electric potential were investigated. Further, the kinetics of veratryl alcohol oxidation in an electrochemical reactor were compared to oxidation when hydrogen peroxide was supplied externally.
Lee
KiBeom
K
Department of Environmental Science and Engineering, Kwangju Institute of Science and Technology, Kwangju, South Korea. klee@wistar.upenn.edu
Moon
Seung-Hyeon
SH
D001547:Benzaldehydes / Q000138:chemical synthesis*; D001592:Benzyl Alcohols / Q000737:chemistry*; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D005240:Feasibility Studies; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000096:biosynthesis / Q000737:chemistry*; D020075:Phanerochaete / Q000737:chemistry* / Q000201:enzymology; D013379:Substrate Specificity; D014867:Water / Q000737:chemistry
D001547:Benzaldehydes; D001592:Benzyl Alcohols; D014867:Water; D010544:Peroxidases; C042858:lignin peroxidase; C042197:veratryl alcohol; C427725:veratraldehyde