Soluble ICAM-1, MCP-1, and MIP-2 protein secretion by rat pleural mesothelial cells following exposure to amosite asbestos.
Pleural inflammation is a sequela of exposure to toxic mineral fibers such as amosite asbestos. This inflammatory response involves the influx of leukocytes from the vasculature into the pleural space. Adhesion molecules such as intercellular adhesion molecule-1 (ICAM)-1 and chemokines such as monocyte chemoattractant protein-1 (MCP)-1 and macrophage inhibitory protein-2 (MIP)-2 are known to be important in pulmonary inflammation following inhalation of particulate matter. However, little is known about their role in pleural inflammation secondary to amosite asbestos exposure. Because the pleural mesothelial cell is believed to be a key target cell of asbestos exposure, the purpose of this study was to determine if ICAM-1, MCP-1, and MIP-2 proteins were secreted by these mesothelial cells following in vitro and in vivo exposure to amosite asbestos. Increased levels of ICAM-1 and MCP-1 protein were measured following 24 or 48 hours exposure of cultured rat pleural mesothelial cells to amosite fibers (1.5 to 5.0 micro g/cm(2)). Increased levels of ICAM-1, MCP-1, and MIP-2 protein were found in pleural lavage fluid from Fischer-344 rats exposed to amosite asbestos for 4 and 12 weeks and after a 12-week recovery period (following the 12-week exposure period). These findings suggest that the secretion of ICAM-1, MCP-1, and MIP-2 by rat pleural mesothelial cells may contribute to amosite-induced pleural inflammation.
Hill
Georgette D
GD
Department of Respiratory Toxicology, CIIT Centers for Health Research, Research Triangle Park, North Carolina 27709, USA. hill9@niehs.nih.gov
Mangum
James B
JB
Moss
Owen R
OR
Everitt
Jeffrey I
JI
D000280:Administration, Inhalation; D000818:Animals; D017639:Asbestos, Amosite / Q000008:administration & dosage / Q000633:toxicity*; D017638:Asbestos, Crocidolite / Q000008:administration & dosage / Q000633:toxicity; D018932:Chemokine CCL2 / Q000378:metabolism*; D054426:Chemokine CXCL2; D019743:Chemokines, CXC; D017077:Culture Media, Conditioned / Q000737:chemistry / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D036341:Intercellular Signaling Peptides and Proteins; D008297:Male; D015846:Monokines / Q000378:metabolism*; D010994:Pleura / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D010996:Pleural Effusion / Q000378:metabolism / Q000473:pathology; D010998:Pleurisy / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D051381:Rats; D011916:Rats, Inbred F344; D013047:Specific Pathogen-Free Organisms
D018932:Chemokine CCL2; D054426:Chemokine CXCL2; D019743:Chemokines, CXC; D017077:Culture Media, Conditioned; C513296:Cxcl2 protein, rat; D036341:Intercellular Signaling Peptides and Proteins; D015846:Monokines; D017638:Asbestos, Crocidolite; D017639:Asbestos, Amosite; D018799:Intercellular Adhesion Molecule-1