[Changes of 5 alpha-reductase type II activity in sexual gland of diabetic male rats].
OBJECTIVE
To study the changes of sexual gland 5 alpha-reductase type II activity in pubertal and adult rats with diabetes.


METHODS
We selected 40 and 90 days old male Wistar rats as pubertal and adult animal model respectively, 30 rats in each group. The rats were randomly divided into three groups: control group (C), diabetic group (D) and diabetes with insulin replacement group (ID). The activity of 5 alpha-reductase type II was measured with thin layer chromatography in the epididymis, prostate and testis.


RESULTS
1. In all sexual glands of pubertal rats, the activity of 5 alpha-reductase type II in D group is significantly lower than that in C and ID groups. 2. In all sexual glands of adult rats. there is no difference in the activity of 5 alpha-reductase type II among these groups.


CONCLUSIONS
The activity of 5 alpha-reductase type II is likely to be influenced by metabolic environment, hormonal levels and local specific factors in pubertal rats, but it is relatively stable in adult rats.
Department of Cell Biology, School of Basic Medical Sciences, Jilin University, Changchun, Jilin 130021, China.
Hong-Jun
HJ
Wang
Jia-Ying
JY
Zhang
Su-Huan
SH
Liu
Hui
H
Zhao
Zhong-Shan
ZS
Wang
D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase / Q000378:metabolism*; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology*; D004822:Epididymis / Q000201:enzymology; D008297:Male; D011467:Prostate / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D013737:Testis / Q000201:enzymology
D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase