Differential effects of apotransferrin on two populations of oligodendroglial cells.
In the central nervous system (CNS), apotransferrin (aTf) is produced by oligodendroglial cells (OLGcs), and aTf is essential for cell survival. We previously demonstrated that a single intracranial injection of aTf in 3-day-old rats accelerates differentiation of OLGc and that aTf acts at early stages of development on certain populations of OLGcs, promoting accelerated maturation, with no effect on late markers of cell differentiation. The objective of the present study was to analyze OLGc maturation at two different stages of rat development, 4 and 10 days of age, in OLGcs isolated from the brain after intracranial injection of aTf at 3 days of age, and to explore the in vitro effect of aTf added to cultures of OLGc isolated from aTf-injected and control brains. The maturational cell stages were identified by immunocytochemistry with different OLGc markers and by analysis of their morphological complexity. The OLGcs isolated from 4- and 10-day-old animals intracranially injected with aTf were more differentiated than control cells. Treatment with aTf of the cultures of OLGcs that were isolated from 4-day-old saline-injected control animals induced their differentiation, while a similar treatment of the cultures of OLGcs that were isolated from 10-day-old animals did not induce further maturation of the cells. The results presented in the present report demonstrate that the in vivo effects of aTf on OLGc maturation can be reproduced in cultures and that the effects of aTf occur early in development during a narrow, transient "temporal window" within which OLGcs are sensitive to its action.
García
C I
CI
Instituto de Química y Fisicoquímica Biológica (UBA-CONICET), Universidad de Buenos Aires, Buenos Aires, Argentina.
Paez
P
P
Soto
E F
EF
Pasquini
J M
JM
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D001059:Apoproteins / Q000494:pharmacology*; D015415:Biomarkers; D001921:Brain / Q000166:cytology / Q000254:growth & development; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D005260:Female; D008297:Male; D009836:Oligodendroglia / Q000166:cytology / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D014168:Transferrin / Q000494:pharmacology*
D001059:Apoproteins; D015415:Biomarkers; D014168:Transferrin; C028284:apotransferrin