Pectate lyase activity during ripening of banana fruit.
Pectate lyase (PEL) activity was demonstrated in ripe banana fruits on supplementing the homogenizing medium with cysteine and Triton X-100. The enzyme was characterized on the basis of alkaline pH optimum, elimination of the activity by EDTA and activation by Ca(2+). PEL activity was not detected in preclimacteric banana fruits. PEL activity increased progressively from early climacteric and reached maximum level at climacteric peak and declined in post climacteric and over ripened fruits. Replacing pectate with pectin in PEL assay manifested enzyme activity even in preclimacteric fruits. In contrast to PEL, polygalacturonase activity progressively increased during fruit ripening even in postclimacteric fruits.
Department of Biochemistry, University of Lucknow, -226 007, Lucknow, India.
Anurag
A
Payasi
G G
GG
Sanwal
D002118:Calcium / Q000494:pharmacology; D019069:Cell Respiration / Q000502:physiology; D004492:Edetic Acid / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D005638:Fruit / Q000201:enzymology* / Q000254:growth & development; D006863:Hydrogen-Ion Concentration; D028521:Musa / Q000201:enzymology* / Q000254:growth & development; D010368:Pectins / Q000378:metabolism; D011096:Polygalacturonase / Q000378:metabolism; D011133:Polysaccharide-Lyases / Q000302:isolation & purification / Q000378:metabolism*; D013379:Substrate Specificity; D013997:Time Factors
D010368:Pectins; D004492:Edetic Acid; D011096:Polygalacturonase; D011133:Polysaccharide-Lyases; C023911:pectate lyase; D002118:Calcium