Chromate tolerance caused by reduced hydroxyl radical production and decreased glutathione reductase activity in Schizosaccharomyces pombe.
The stable Cr(VI)-tolerant chr1-66T mutant of Schizosaccharomyces pombe, which carries one simple gene mutation responsible for Cr(VI) tolerance, accumulated and reduced the chromate anion (CrO(4)(2-)) significantly more slowly than did its parental strain 6chr(+). The mutant chr1-66T proved to be sensitive to oxidative stressors such as H(2)O(2), menadione, tert-butyl hydroperoxide and Cd(2+). Both the Cr(VI) tolerance and the oxidative stress sensitivity were attributed to a decreased specific glutathione reductase activity. These effects were also enhanced with a decrease in the specific mitochondrial Mn-SOD activity.
Gazdag
Zoltán
Z
Department of General and Environmental Microbiology, University of Pécs, Hungary.
Pócsi
István
I
Belágyi
József
J
Emri
Tamás
T
Blaskó
Agnes
A
Takács
Krisztina
K
Pesti
Miklós
M
D002840:Chromates / Q000494:pharmacology*; D025141:Drug Resistance, Fungal; D005980:Glutathione Reductase / Q000378:metabolism*; D017665:Hydroxyl Radical / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000187:drug effects* / Q000378:metabolism
D002840:Chromates; D017665:Hydroxyl Radical; D005980:Glutathione Reductase