Quantitative analysis of hippostasin/KLK11 gene expression in cancerous and noncancerous prostatic tissues.
OBJECTIVE
Hippostasin/kallikrein 11 (KLK11) is a member of the human kallikrein gene family, which includes prostate-specific antigen (PSA), human kallikrein 2 (hK2), and another 12 members, all localized on chromosome 19q13.4. Hippostasin has two alternative splicing isoforms, known as the brain type and prostate type. We have previously reported that the prostate-type isoform is not expressed in human prostate cancer cell lines.


METHODS
We compared the expression of hippostasin/KLK11 isoforms in 76 matched pairs of human normal and prostate cancer tissues by quantitative reverse transcriptase-polymerase chain reaction.


RESULTS
The expression of both isoforms of KLK11 was 25% to 45% higher in cancer tissues compared with their normal counterparts. Regarding prostate-type KLK11, we identified a significant association between lower expression and higher tumor stage, Gleason score, and tumor grade. No such association was seen with the brain-type isoform.


CONCLUSIONS
: The expression of the prostate-type isoform of KLK11 is increased in prostate cancer. This parameter should be examined further as a new prognostic indicator of prostate cancer.
Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, Ontario, Canada.
Terukazu
T
Nakamura
Carsten
C
Stephan
Andreas
A
Scorilas
George M
GM
Yousef
Klaus
K
Jung
Eleftherios P
EP
Diamandis
D000230:Adenocarcinoma / Q000201:enzymology* / Q000235:genetics* / Q000601:surgery; D000368:Aged; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011467:Prostate / Q000737:chemistry* / Q000201:enzymology* / Q000378:metabolism / Q000601:surgery; D011468:Prostatectomy; D011471:Prostatic Neoplasms / Q000201:enzymology* / Q000235:genetics* / Q000601:surgery; D012697:Serine Endopeptidases / Q000235:genetics*
D007527:Isoenzymes; C114880:trypsin-like serine protease; D012697:Serine Endopeptidases