Recruitment of HIV and its receptors to dendritic cell-T cell junctions.
Monocyte-derived dendritic cells (MDDCs) can efficiently bind and transfer HIV infectivity without themselves becoming infected. Using live-cell microscopy, we found that HIV was recruited to sites of cell contact in MDDCs. Analysis of conjugates between MDDCs and T cells revealed that, in the absence of antigen-specific signaling, the HIV receptors CD4, CCR5, and CXCR4 on the T cell were recruited to the interface while the MDDCs concentrated HIV to the same region. We propose that contact between dendritic cells and T cells facilitates transmission of HIV by locally concentrating virus, receptor, and coreceptor during the formation of an infectious synapse.
Department of Microbiology and Immunology, University of Illinois, Chicago, IL 60612, USA.
David
D
McDonald
Li
L
Wu
Stacy M
SM
Bohks
Vineet N
VN
KewalRamani
Derya
D
Unutmaz
Thomas J
TJ
Hope
D015704:CD4 Antigens / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology / Q000648:ultrastructure / Q000821:virology*; D002448:Cell Adhesion; D002462:Cell Membrane / Q000821:virology; D002478:Cells, Cultured; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000502:physiology / Q000648:ultrastructure / Q000821:virology*; D005455:Fluorescent Antibody Technique; D016336:Gene Products, vpr / Q000032:analysis; D049452:Green Fluorescent Proteins; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D007365:Intercellular Junctions / Q000502:physiology / Q000821:virology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008156:Luciferases / Q000032:analysis; D008164:Luminescent Proteins / Q000032:analysis; D008954:Models, Biological; D009000:Monocytes; D019713:Receptors, CCR5 / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000378:metabolism; D015728:Receptors, HIV / Q000378:metabolism*; D014771:Virion / Q000502:physiology; D054325:vpr Gene Products, Human Immunodeficiency Virus
D015704:CD4 Antigens; D016336:Gene Products, vpr; D008070:Lipopolysaccharides; D008164:Luminescent Proteins; D019713:Receptors, CCR5; D019718:Receptors, CXCR4; D015728:Receptors, HIV; D054325:vpr Gene Products, Human Immunodeficiency Virus; D049452:Green Fluorescent Proteins; D008156:Luciferases