Development of a specific monoclonal antibody for grouper (Epinephelus guaza) identification by an indirect enzyme-linked immunosorbent assay.
Identification of fish species adulteration is important for consumer protection and the enforcement of food-labeling laws. A monoclonal antibody (MAb) generated against soluble muscle proteins from grouper (Epinephelus guaza) has been used in two indirect enzyme-linked immunosorbent assay (ELISA) formats (microtiter plates and immunostick tubes) for the rapid authentication of grouper fillets. The 3D12 MAb was produced with the use of the hybridoma technique and tested against several commonly consumed fish species by ELISA. The 3D12 MAb specifically reacted with grouper samples and could be useful for the discrimination of grouper among other, less-valued, fish species sold in the marketplace.
Asensio
Luis
L
Departamento de Nutrición y Bromatología III (Higiene y Tecnologiá de los Alinmntos), Facultad de Veteirnaria, Universidad Complutense, 28040 Madrid, Spain.
González
Isabel
I
Rodríguez
Miguel A
MA
Mayoral
Belén
B
Lopez-Calleja
Inés
I
Hernández
Pablo E
PE
García
Teresa
T
Martín
Rosario
R
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D005506:Food Contamination / Q000517:prevention & control; D005515:Food Labeling; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D010473:Perciformes / Q000145:classification* / Q000276:immunology; D017747:Seafood / Q000145:classification* / Q000592:standards; D013045:Species Specificity
D000911:Antibodies, Monoclonal