Continuous cell-free protein synthesis directed by messenger DNA and catalyzed by extract of Thermus thermophilus HB27.
Polypeptide synthesis directed by DNA as the messenger in a cell-free system of Thermus thermophilus was investigated. Polypeptides were synthesized with the addition of neomycin in the presence of DNA catalyzed by the cell extract. The stability of messenger DNA was greater than that of messenger RNA. Continuous cell-free translation with messenger DNA produced polypeptides at the rate of more than 8 microg/h in the presence of spermine.
Department of Life Science, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama 226-8501, Japan. uzawa@cc.osaka-kyoiku.ac.jp
Taketoshi
T
Uzawa
Akihiko
A
Yamagishi
Tairo
T
Oshima
D002384:Catalysis; D002474:Cell-Free System; D004247:DNA / Q000737:chemistry / Q000187:drug effects / Q000502:physiology*; D009355:Neomycin / Q000494:pharmacology; D010452:Peptide Biosynthesis / Q000187:drug effects / Q000502:physiology*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism; D013096:Spermine / Q000494:pharmacology; D016963:Thermus thermophilus / Q000378:metabolism*
D012333:RNA, Messenger; D013096:Spermine; D004247:DNA; D009355:Neomycin