Isolation of Paenibacillus illinoisensis that produces cyclodextrin glucanotransferase resistant to organic solvents.
A bacterium that secreted cyclodextrin glucanotransferase (CGTase) in a medium overlaid with n-hexane was isolated and identified as Paenibacillus illinoisensis strain ST-12 K. The CGTase of the strain was purified from the culture supernatant. The molecular mass was 70 kDa. The enzyme was stable at pH 6 to 10 and active at pH 5.0 to 8.0. The optimum temperature at pH 7.0 was 65 degrees C in the presence of 5 mM CaCl2. The enzyme produced mainly beta-cyclodextrin. The total yield of alpha-, beta-, and gamma- cyclodextrins was increased 1.4-fold by the addition of ethanol. In particular, the yield of beta-cyclodextrins in the presence of 10% (vol/vol) ethanol was 1.6-fold that without ethanol. The CGTase was stable and active in the presence of large amounts of various organic solvents.
Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta 4259, Midori-ku, Yokohama 226-8501, Japan. ndoukyu@bio.titech.ac.jp
Noriyuki
N
Doukyu
Hirokazu
H
Kuwahara
Rikizo
R
Aono
D001407:Bacillus / Q000145:classification / Q000201:enzymology / Q000302:isolation & purification*; D003505:Cyclodextrins / Q000032:analysis / Q000096:biosynthesis; D004795:Enzyme Stability; D005964:Glucosyltransferases / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009930:Organic Chemicals; D012997:Solvents; D013696:Temperature
D003505:Cyclodextrins; D009930:Organic Chemicals; D012997:Solvents; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase