Inhibitory effect of TNF-alpha and IL-8 secretion by pimarane-type diterpenoids from Acanthopanax koreanum.
A new pimarane-type diterpene compound, acanthokoreoic acid A together with three known compounds, acanthoic acid, acanthol, and sumogaside were isolated from a CH(2)Cl(2) fraction of Acanthopanax koreanum by repeated column chromatography and reversed phase preparative HPLC. Acanthoic acid was isolated in high yields and showed potent inhibitory activity on the IL-8 secretion of the TNF-alpha-stimulated human colon adenocarcinoma cell line HT-29 and on the TNF-alpha secretion of the trypsin-stimulated human leukemic mast cell line HMC-1.
Cai
Xing Fu
XF
College of Pharmacy, Chungnam National University, Daejeon, Korea.
Shen
Guanghai
G
Dat
Nguyen Tien
NT
Kang
Ok Hwa
OH
Kim
Jin A
JA
Lee
Young Mi
YM
Lee
Jung Joon
JJ
Kim
Young Ho
YH
D045784:Abietanes; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D028001:Eleutherococcus / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D019073:HT29 Cells; D006801:Humans; D016209:Interleukin-8 / Q000037:antagonists & inhibitors* / Q000378:metabolism; D007723:Korea; D009682:Magnetic Resonance Spectroscopy; D008407:Mast Cells / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013055:Spectrophotometry, Infrared; D014357:Trypsin / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000378:metabolism
D045784:Abietanes; D004224:Diterpenes; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha; C479949:acanthokoreoic acid A; C433018:pimarane; D014357:Trypsin