Structural elucidation and antitumor activity of a fructan from Cyathula officinalis Kuan.
A fructan named CoPS3 was isolated from Cyathula officinalis Kuan. The structure of CoPS3 was determined by methylation, by the reductive-cleavage method combined with GC-MS analysis, and both 1D and 2D 1H and 13C NMR spectroscopy. These results show that CoPS3 is a graminans-type fructan that is comprised of a beta-D-fructofuranosyl backbone having residues linked (2-->1)- and (2-->6) with branches and an alpha-D-glucopyranose residue on the nonreducing end of the fructan chain. Each branch is terminated by a beta-D-Fruf residue. Bioassay showed that it could inhibit growth of Lewis pulmonary carcinoma implanted in mice.
State Key Laboratory of Bio-organic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Academia Sinica, 200032, Shanghai, PR China.
Xiao-Ming
XM
Chen
Geng-Yuan
GY
Tian
D027703:Amaranthaceae / Q000737:chemistry*; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology; D018827:Carcinoma, Lewis Lung / Q000473:pathology / Q000517:prevention & control; D045744:Cell Line, Tumor; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology; D005630:Fructans / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D007274:Injections, Intraperitoneal; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008745:Methylation; D051379:Mice; D008810:Mice, Inbred C57BL
D000970:Antineoplastic Agents; C481523:CoPS3 fructan, Cyanthula officinalis; D004365:Drugs, Chinese Herbal; D005630:Fructans