A new mannoheptaose containing alpha and beta-(1-->2) linkages isolated from the mannan of Torulaspora delbrueckii: ELISA inhibition studies.
Torulaspora delbrueckii starin IFO 0955 was examined with respect to its structural and serological properties of the cell wall mannan (Td-0955-M). Td-0955-M revealed significant reactivities with sera from a commercially available factor serum kit (Candida Check) in ELISA. Td-0955-M was investigated for its chemical structure by acetolysis under conventional and mild conditions. NMR and GC techniques were used as analytical techniques. The mannooligosaccharide fractions eluted from a Bio-Gel P-2 column were found to consist of Man(alpha1-2)Man, M2, Man(alpha1-2)Man(alpha1-2)Man and Man(beta1-2)Man(alpha1-2)Man, M3, Man(alpha1-2)Man(beta1-2)Man(beta1-2)Man(alpha1-2)Man, M5, and a new mannoheptaose, which possesses the structure, Man(alpha1-2)Man(beta1-2)Man(beta1-2)Man(beta1-2)Man(beta1-2)Man(alpha1-2)Man, M7. The results of the inhibition ELISA showed that the M7 oligosaccharide significantly inhibited the reactivities in the Td-0955-M-factor serum systems.
Second Department of Hygienic Chemistry, Tohoku Pharmaceutical University, 4-4-1 Komatsushima, Sendai Aoba-ku, 981-8558, Miyagi, Japan. okawa@tohoku-pharm.ac.jp
Yoshio
Y
Okawa
Kouichi
K
Monma
Nobuyuki
N
Shibata
Hidemitsu
H
Kobayashi
Yasuyo
Y
Yamada
D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000737:chemistry* / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification; D004718:Saccharomycetales / Q000737:chemistry* / Q000145:classification; D012703:Serotyping
D008351:Mannans; D009844:Oligosaccharides; C481522:mannoheptaose, Torulaspora delbrueckii