A new dark quencher for use in genetic analysis.
A novel, long-wavelength, non-fluorescent quencher (LQ), based on 1,4-diaminoanthraquinone, has been incorporated at the 3' and 5'-termini of oligonucleotides. The quencher has been used in Molecular beacons, efficiently quenching the long wavelength fluorophore, Cy5.
Department of Chemistry, University of Southampton, Highfield, Southampton, UK SO17 1BJ.
Jonathan P
JP
May
Lynda J
LJ
Brown
Ivo
I
Rudloff
Tom
T
Brown
D000880:Anthraquinones / Q000737:chemistry*; D002232:Carbocyanines / Q000737:chemistry; D005454:Fluorescence Polarization; D005821:Genetic Techniques; D015345:Oligonucleotide Probes / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods
D000880:Anthraquinones; D002232:Carbocyanines; D015345:Oligonucleotide Probes; D009841:Oligonucleotides; C085321:cyanine dye 5; C039689:1,4-diaminoanthraquinone