Protocol for rapid fluorescence in situ hybridization of bacteria in cryosections of microarthropods.
A protocol was developed to detect bacteria inhabiting microarthropods by means of small-subunit rRNA-targeted fluorescence in situ hybridization and microscopy. The protocol is based on cryosections of whole specimens. In contrast to more commonly applied paraffin-embedding techniques, the protocol is quicker and reduces the number of manipulations which might damage the microscopic material. The method allowed the study of the bacterial colonization of Folsomia candida (Collembola) and the detection of bacteria in both the gut and tissue.
Thimm
Torsten
T
Institut für Agrarökologie, Bundesforschunganstalt für Landwirtschaft (FAL), Bundesallee 50, D-38116 Braunschweig, Germany.
Tebbe
Christoph C
CC
D000818:Animals; D001181:Arthropods / Q000382:microbiology*; D001419:Bacteria / Q000235:genetics* / Q000302:isolation & purification*; D001483:Base Sequence; D015342:DNA Probes / Q000235:genetics; D004064:Digestive System / Q000382:microbiology; D005629:Frozen Sections; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods
D015342:DNA Probes