[Changes in the lipid physical state in human erythrocyte membranes subjected lead exposure in vitro].
The effect of lead acetate on the physical state of membrane lipids in human erythrocytes in vitro was studied using the lipophilic fluorescence probe 1,6-diphenyl-1,3,5-hexatriene and spin probes 16-doxyl-stearate and iminoxyl palmitic acid. It was shown that 2-10 microM lead acetate causes an increase in both intensity and polarization of fluorescence of 1,6-diphenyl-1,3,5-hexatriene, indicating changes in the microviscosity of the lipid bilayer of erythrocyte membranes. Judging from the parameters of EPR spectra of 16-doxyl stearate and iminoxyl palmitic acid incorporated into erythrocyte membranes, 2-10 microM lead acetate increases the heterogeneity of the lipid bilayer in surface and deep hydrophobic layers of the erythrocyte membrane.
Oleksiuk
O B
OB
Institute of Photobiology, National Academy of Sciences of Belarus, ul. Akademicheskaya 27, Minsk, 220072 Belarus.
Finin
V S
VS
Slobozhanina
E I
EI
D003497:Cyclic N-Oxides; D004161:Diphenylhexatriene; D004578:Electron Spin Resonance Spectroscopy; D004785:Environmental Pollutants / Q000633:toxicity*; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000187:drug effects*; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes; D006801:Humans; D066298:In Vitro Techniques; D008563:Membrane Lipids / Q000737:chemistry*; D009942:Organometallic Compounds / Q000633:toxicity*; D010169:Palmitic Acids; D013050:Spectrometry, Fluorescence; D013113:Spin Labels
D003497:Cyclic N-Oxides; D004785:Environmental Pollutants; D005456:Fluorescent Dyes; D008563:Membrane Lipids; D009942:Organometallic Compounds; D010169:Palmitic Acids; D013113:Spin Labels; D004161:Diphenylhexatriene; C032363:16-nitroxystearic acid; C008261:lead acetate