Modes of programmed cell death during Ceratitis capitata oogenesis.
In the present study, we demonstrate the existence of two distinct apoptotic patterns in nurse cells during Ceratitis capitata oogenesis. One is developmentally regulated and normally occurs during stages 12 and 13, and the other is stage specific and is sporadically observed during stages 7 and 8. The pre-apoptotic manifestation of the first pattern begins at stage 11 and is characterized by the formation of actin bundles. Subsequently, at stages 12 and 13, the nurse cell nuclei exhibit condensed chromatin and contain fragmented DNA, as revealed by TUNEL assay. The apoptotic nurse cell remnants are phagocytosed by the neighboring follicle cells at the end of oogenesis during stages 13 and 14. In the second apoptotic pattern, which occurs sporadically during stages 7 and 8, the nurse cells degenerate and are phagocytosed by the follicular epithelium that contains apoptotic cell bodies. The data presented herein, compared to previous reported results in Drosophila melanogaster and Dacus oleae (Nezis et al., 2000, 2001), strongly suggest that nurse cell apoptosis is a developmentally regulated and phylogenetically conserved mechanism in higher Dipteran. They also suggest that, the sporadic apoptotic pattern consists of a possible protective mechanism throughout oogenesis when damaged or abnormal egg chambers, are eliminated before they reach maturity.
Faculty of Biology, Department of Cell Biology and Biophysics, University of Athens, Panepistimiopolis 15784, Athens, Greece.
Ioannis P
IP
Nezis
Vassilis
V
Modes
Vicky
V
Mpakou
Dimitrios J
DJ
Stravopodis
Issidora S
IS
Papassideri
Ioanna
I
Mammali
Lukas H
LH
Margaritis
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D033622:Ceratitis capitata / Q000502:physiology*; D003599:Cytoskeleton / Q000648:ultrastructure; D005260:Female; D020287:In Situ Nick-End Labeling; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D009866:Oogenesis / Q000502:physiology*; D010053:Ovary / Q000502:physiology / Q000648:ultrastructure