Optimisation of interdelta analysis for Saccharomyces cerevisiae strain characterisation.
A new primer pair (delta12-delta21) for polymerase chain reaction-based yeast typing was designed using the yeast genome sequence. The specificity of this primer pair was checked by the comparison of the electrophoresis pattern with a virtual profile calculated from Blast data. The analysis of 53 commercial and laboratory Saccharomyces cerevisiae yeast strains showed a clear improvement of interdelta analysis using the newly designed primers.
Legras
Jean-Luc
JL
Institut National de la Recherche Agronomique (INRA), Unité Mixte de Recherche Vigne et Vins d'Alsace, Colmar, France. legras@colmar.inra.fr
Karst
Francis
F
D001483:Base Sequence; D017931:DNA Primers; D004271:DNA, Fungal / Q000032:analysis; D016208:Databases, Factual; D004586:Electrophoresis / Q000379:methods; D014644:Genetic Variation; D016681:Genome, Fungal; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000145:classification* / Q000235:genetics; D017422:Sequence Analysis, DNA; D020079:Terminal Repeat Sequences / Q000235:genetics*
D017931:DNA Primers; D004271:DNA, Fungal