Dental amalgam as one of the risk factors in autoimmune diseases.
BACKGROUND
Experimental and clinical data published recently show that dental amalgam can give rise to undesirable immunological responses in susceptible individuals. In genetically susceptible strains of experimental animals, mercury and silver can induce autoimmune responses. Sera of patients sensitive to mercury were found to have a higher incidence of autoantibodies relative to controls.


OBJECTIVE
The aim of this study was to determine possible presence of antinuclear SSB/La autoantibodies after the in vitro stimulation of peripheral blood lymphocytes with HgCl2.


METHODS
Lymphocytes were obtained from patients with autoimmune thyroiditis and increased response to mercury in vitro. Mononuclear cells were cultivated for 6 days with 100 microl HgCl2 solution or with pure medium and the levels of antinuclear autoantibodies SSB/La were assayed by a commercial SSB/La ELISA kit.


RESULTS
Increased production of SSB/La autoantibodies in the media following stimulation of peripheral blood lymphocytes with HgCl2 was found in all cases. Using the Student's paired test, the results were significant on the p=0.05 significance level.


CONCLUSIONS
Results imply that, in some patients with thyroiditis, mercury from dental amalgam can stimulate the production of antinuclear antibodies. Dental amalgam may be a risk factor in some patients with autoimmune disease.
Institute of Dental Research, lst Medical Faculty, Charles University and General Faculty Hospital Prague, Vinohradská 48, 120 60 Prague 2, Czech Republic. jirina.bartova@post.cz
Jirina
J
Bártová
Jarmila
J
Procházková
Zuzana
Z
Krátká
Klára
K
Benetková
Zora
Z
Venclíková
Ivan
I
Sterzl
D000974:Antibodies, Antinuclear / Q000032:analysis; D001323:Autoantibodies / Q000032:analysis; D001327:Autoimmune Diseases / Q000453:epidemiology / Q000209:etiology*; D003723:Dental Amalgam / Q000009:adverse effects*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D008627:Mercuric Chloride / Q000494:pharmacology; D012307:Risk Factors; D013967:Thyroiditis, Autoimmune / Q000097:blood / Q000276:immunology
D000974:Antibodies, Antinuclear; D001323:Autoantibodies; D008627:Mercuric Chloride; D003723:Dental Amalgam