New gastric epithelial cell lines from mice transgenic for temperature-sensitive simian virus 40 large T antigen show distinct types of cell differentiation.
OBJECTIVE
To develop conditionally immortalized gastric mucosal cell lines that show distinct types of cell differentiation from transgenic mice harboring temperature-sensitive simian virus 40 (tsSV40) large T antigen.


METHODS
Gastric mucosal cells from the transgenic mice were cultured at a permissive temperature (33 degrees C), and proliferative cells were then cloned by colony formation.


RESULTS
Eight gastric cell lines showed epithelial-like morphology and grew at 33 degrees C. Three different types of the cell lines have been established: (1) MGE12-1, MGE3-2, and MGE509 cells expressing mRNAs for pit cell markers (gastric mucin and cathepsin E); (2) MGE02, MGE503, and MGE511 cells expressing mRNAs for pit and zymogenic (pepsinogen F) cell markers, and (3) MGE507 and MGE727 cells expressing mRNAs for pit, zymogenic, and parietal (H,K-ATPase alpha-subunit) cell markers. Moreover, the TaqMan assay showed that mRNA levels of mucin, H,K-ATPase alpha-subunit, and pepsinogen F were influenced by nonpermissive temperature (39 degrees C) in MGE503 and MGE727 cells.


CONCLUSIONS
These gastric epithelial cell lines seem to reflect different stages of development of gastric mucosal cells.
Molecular Genetics Research Center, Toyama Medical and Pharmaceutical University, Toyama, Japan. ytabu@ms.toyama-mpu.ac.jp
Yoshiaki
Y
Tabuchi
Yuko
Y
Arai
Hiroki
H
Shioya
Ryosuke
R
Kuribayashi
Kotaro
K
Ishibashi
Norifumi
N
Sugiyama
Masuo
M
Obinata
Noriaki
N
Takeguchi
Shinji
S
Asano
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000378:metabolism*; D015415:Biomarkers; D015153:Blotting, Western; D020059:Cathepsin E / Q000235:genetics; D002454:Cell Differentiation / Q000502:physiology*; D002455:Cell Division; D002461:Cell Line, Transformed / Q000502:physiology; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D005260:Female; D005752:Gastric Mucins / Q000235:genetics; D005753:Gastric Mucosa / Q000166:cytology* / Q000378:metabolism; D017506:H(+)-K(+)-Exchanging ATPase / Q000235:genetics; D051379:Mice; D008819:Mice, Nude; D008822:Mice, Transgenic; D010435:Pepsinogens / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013696:Temperature
D000952:Antigens, Polyomavirus Transforming; D015415:Biomarkers; D005752:Gastric Mucins; D010435:Pepsinogens; D012333:RNA, Messenger; D020059:Cathepsin E; D017506:H(+)-K(+)-Exchanging ATPase