Cloning and characterization of porcine common gamma chain gene.
The common gamma chain, which was originally identified as a component of interleukin-2 receptors (IL-2R), plays a key role in differentiation of T lymphocytes and natural killer (NK) cells. In the present study, cDNA of the porcine common gamma chain gene and its genomic DNA were molecularly cloned and characterized. The porcine common gamma chain gene was found to consist of 8 exons, spanning approximately 3.7 kb, and to encode a 368-amino acid polypeptide. The amino acid sequence showed 82.4%, 71.1%, 86.1%, and 84.8% similarities with that of human, murine, bovine, and canine chains, respectively. The common gamma chain gene was assigned to swine chromosome Xq13 by FISH analysis and was consistent with the result of radiation hybrid (RH) mapping. When various porcine tissues were examined for the expression of this gene, the expression was observed in lymphocytes and lymphocyte-related tissues. Since GATA, T cell factor-1 (TCF-1), Ets-1, activated protein2 (AP-2), and Ikaros2 binding motifs were demonstrated in the 5' upstream region of this gene, promoter activity was investigated using luciferase gene as a reporter. The results indicate that the Ets-1 binding motif in the segment from -95 to -59 (major transcription initiation site: +1) was an essential cis-acting regulatory element for the common gamma chain gene in lymphoid cells.
Honma
Daisuke
D
Genome Research Department, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-0901, Japan.
Uenishi
Hirohide
H
Hiraiwa
Hideki
H
Watanabe
Satoshi
S
Tang
Weiran
W
Kiyokawa
Nobutaka
N
Fujimoto
Junichiro
J
Yasue
Hiroshi
H
Sakimura
Kenji
K
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons; D005260:Female; D017930:Genes, Reporter; D016678:Genome; D008214:Lymphocytes / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011401:Promoter Regions, Genetic; D017434:Protein Structure, Tertiary; D015375:Receptors, Interleukin-2 / Q000737:chemistry / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D017386:Sequence Homology, Amino Acid; D013552:Swine; D014158:Transcription, Genetic
D018076:DNA, Complementary; D015375:Receptors, Interleukin-2