Gene delivery to the lung by means of syngeneic fibroblasts: an experimental model.
An experimental model, in which exogenous gene expression in the lung is achieved, has been established. A fibroblast cell line was transfected with the lacZ gene and was administered to syngeneic mice by either intravenous or intratracheal injection. Enzyme-linked immunosorbent assay and histochemical detection of beta-galactosidase revealed efficient gene delivery to the lung by either route. Kinetic studies demonstrated that the expression peaked immediately after the injection, and this high level was maintained for 7 days by intratracheal and for 14 days by intravenous administration. This system may have potential relevance for certain experimental models requiring specific gene delivery to the lung.
Suda
Akira
A
Department of Respirology (B2), Graduate School of Medicine, Chiba University, Japan.
Takiguchi
Yuichi
Y
Omori
Shigenari
S
Miyazawa
Hiroshi
H
Sugimoto
Takaaki
T
Kurosu
Katsushi
K
Tatsumi
Koichiro
K
Tanabe
Nobuhiro
N
Kuriyama
Takayuki
T
Hiroshima
Kenzo
K
Kimura
Hiroshi
H
D000818:Animals; D017690:Cell Transplantation; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000201:enzymology* / Q000637:transplantation; D005696:Galactosidases / Q000235:genetics* / Q000378:metabolism; D018014:Gene Transfer Techniques; D022761:Gene Transfer, Horizontal; D005822:Genetic Vectors; D007763:Lac Operon / Q000235:genetics; D008168:Lung / Q000166:cytology / Q000201:enzymology*; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D013047:Specific Pathogen-Free Organisms; D014162:Transfection
D005696:Galactosidases