Isolation of exfoliative toxin from Staphylococcus intermedius and its local toxicity in dogs.
A rounding effect was demonstrated in cultured cells inoculated with the culture filtrates (CFs) of 60 strains of Staphylococcus intermedius derived from dogs affected with pyoderma. Exfoliative toxin (ET)-like toxin (ETLT) was isolated from the CF of S. intermedius strain D-52, which exhibited strong rounding activity and then was purified by gel filtration on a Sephadex G-75 column, and by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The ETLT caused exfoliation in 1-day-old chickens, suckling Syrian hamsters, and dogs, but not in suckling mice. The ETLT was serologically different from exfoliative toxin A (ETA), exfoliative toxin B (ETB), exfoliative toxin C (ETC), S. hyicus exfoliative toxin A (SHETA), and SHETB, as shown by Western blot analysis. The molecular weight of the ETLT was estimated at 30 kDa by SDS-PAGE. In the present study, we propose the ETLT was a novel type of ET, S. intermedius exfoliative toxin (SIET).
Department of Veterinary Microbiology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori 034-8628, Japan.
Rie
R
Terauchi
Hisaaki
H
Sato
Takako
T
Hasegawa
Takeshi
T
Yamaguchi
Chikara
C
Aizawa
Nobutoshi
N
Maehara
D000818:Animals; D000907:Antibodies, Bacterial / Q000378:metabolism; D001427:Bacterial Toxins / Q000302:isolation & purification* / Q000633:toxicity*; D015153:Blotting, Western / Q000662:veterinary; D002645:Chickens; D002850:Chromatography, Gel / Q000662:veterinary; D006224:Cricetinae; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005083:Exfoliatins / Q000302:isolation & purification* / Q000633:toxicity*; D005260:Female; D008647:Mesocricetus; D051379:Mice; D008807:Mice, Inbred BALB C; D011711:Pyoderma / Q000382:microbiology / Q000662:veterinary*; D051381:Rats; D013047:Specific Pathogen-Free Organisms; D013207:Staphylococcal Skin Infections / Q000378:metabolism / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D013210:Staphylococcus / Q000737:chemistry / Q000378:metabolism*
D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D005083:Exfoliatins