Cytotoxic and multidrug resistance reversal activity of a vegetable, 'Anastasia Red', a variety of sweet pepper.
The vegetable, Anastasia Red, Capsicum annuum L. var. angulosum Mill. (Solanaceae) was successively extracted with hexane, acetone, methanol and 70% methanol, and the extracts were further separated into a total of 21 fractions by silica gel or octadecylsilane (ODS) column chromatography. The biological activities of extracts and fractions were determined. These extracts showed relatively higher cytotoxic activity against two human oral tumor cell lines (HSC-2, HSG) than against normal human gingival fibroblasts (HGF), suggesting a tumor-specific cytotoxic activity. The cytotoxic activity of these extracts was enhanced by fractionation on silica gel [H2, A2, M1-M3] or ODS column chromatography [70M]. Several fractions [H2, H4, H5, A1, A2, A3, A5, A6, A7, M2] reversed the multidrug resistance (MDR) phenotype with L5178 mouse lymphoma T cells, more efficiently than (+/-)-verapamil. The extracts and fractions did not show any detectable anti-human immunodeficiency virus (HIV) or anti-Helicobacter pylori activity. Thus, this study suggests the effective and selective antitumor potential of 'Anastasia Red' of sweet pepper for further phytochemical and biological investigation.
Meiji Pharmaceutical University, Kiyose, Tokyo, Japan. motohasi@my-pharm.ac.jp
Noboru
N
Motohashi
Hidetsugu
H
Wakabayashi
Teruo
T
Kurihara
Yuko
Y
Takada
Shichiro
S
Maruyama
Hiroshi
H
Sakagami
Hideki
H
Nakashima
Satoru
S
Tani
Yoshiaki
Y
Shirataki
Masami
M
Kawase
Kristina
K
Wolfard
Joseph
J
Molnár
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D002212:Capsicum; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D005347:Fibroblasts / Q000187:drug effects*; D005881:Gingiva / Q000187:drug effects*; D006678:HIV / Q000187:drug effects; D016480:Helicobacter pylori / Q000187:drug effects; D006801:Humans; D016399:Lymphoma, T-Cell / Q000188:drug therapy; D051379:Mice; D008826:Microbial Sensitivity Tests; D009062:Mouth Neoplasms / Q000188:drug therapy; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts