[Changes in the expression of membrane markers and in the number of human blood monocytes after single and repeated courses of visible and infrared light at therapeutic doses].
An attempt has been made to prove that the immunomodulating effect of therapeutic doses of polychromatic visible + infrared polarized (VIP) light at its application to a small body surface area is connected with a transcutaneous photomodification of a small amount of blood in superficial skin microvessels. For this purpose, in parallel experiments, using monoclonal antibodies, the membrane phenotype of circulating blood mononuclears was studied after irradiation of volunteers, of samples of their blood in vitvo, and of a mixture of the irradiated and non-irradiated autologous blood in a 1:10 volume ratio, thereby modeling events in vivo, when a small amount of the transcutaneously photomodified blood in the vascular bed contacts its main circulating volume. In this variant of experiment, a great similarity has been established of changes in expression of mononuclear membrane markers (CD3, CD4, CD8, CD20, CD16, HLA-DR and to a lesser degree of CD25); the ability has been proven of the photomodified blood to "translate" the light-induced changes to a much higher volume of non-irradiated blood, which might represent a mechanism of the systemic immunomodulating effect of phototherapy. Under conditions in vivo and in vitro, the most "reactive" were HLA-DR+, CD20+, CD16+, CD4+, and 0-cells. An increase of the total number of lymphocytes and monocytes has been shown by the end of the 10-day-long phototherapeutic course. The regulatory character of the single and course sessions of the VIP light on the blood immunocompetent cells is substantiated: depending on the initial state of the immune system, the VIP light can produce both stimulating and inhibitory effect on lymphoid cell subpopulations, which opens large possibilities of using this method for correction of immunological disturbances in diseases of different etiopathogenesis.
Institute of Cytology RAS, St. Petersburg.
N A
NA
Zhevago
K A
KA
Samoĭlova
T V
TV
Glazanova
K D
KD
Obolenskaia
M F
MF
Balliuzek
N Iu
NIu
Romanenko
D000911:Antibodies, Monoclonal / Q000378:metabolism; D015703:Antigens, CD / Q000032:analysis; D015415:Biomarkers / Q000097:blood*; D001769:Blood / Q000528:radiation effects; D001772:Blood Cell Count; D001804:Blood Transfusion, Autologous; D002462:Cell Membrane / Q000378:metabolism*; D002478:Cells, Cultured; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000528:radiation effects*; D008027:Light; D008213:Lymphocyte Activation / Q000528:radiation effects; D016131:Lymphocyte Subsets / Q000166:cytology / Q000502:physiology; D008214:Lymphocytes / Q000528:radiation effects; D010641:Phenotype; D010789:Phototherapy; D011879:Radiotherapy Dosage; D013997:Time Factors; D014466:Ultraviolet Rays
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015415:Biomarkers; D006684:HLA-DR Antigens