Complete nucleotide sequence of a native plasmid from Brevibacterium linens.
Brevibacterium linens has commercial significance in the dairy industry and potential application in the production of bacteriocins and carotenoids. Strain development of these industrially significant organisms would be facilitated by the use of vectors, yet few are available. In this study we report the isolation of four novel plasmids from the Gram-positive coryneform B. linens, and determine the first complete nucleotide sequence of a native plasmid of B. linens. The cryptic plasmid pLIM is 7610 bp in length, and belongs to a subfamily of theta replicating ColE2-related plasmids. Initial investigation suggests that replication in pLIM requires two replicases, a primase (RepA) and a DNA binding protein (RepB), encoded by a single operon repAB. The origin of replication is located upstream of repAB transcription.
School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney 2052, Australia.
Mathew
M
Moore
Charles
C
Svenson
David
D
Bowling
Dianne
D
Glenn
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D001951:Brevibacterium / Q000235:genetics*; D004265:DNA Helicases; D004261:DNA Replication; D004268:DNA-Binding Proteins; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010957:Plasmids / Q000235:genetics* / Q000302:isolation & purification; D011506:Proteins / Q000235:genetics; D018741:Replication Origin; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D015534:Trans-Activators
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D011506:Proteins; C048817:RepB protein, Bacteria; D015534:Trans-Activators; C045264:replication initiator protein; D004265:DNA Helicases