[Restricted microcirculation disorder induced by photochemical reaction in the guinea pig cochlea].
OBJECTIVE
To investigate the effect of restricted cochlear microcirculation disorder on the cochlear blood flow, auditory function, and morphology.


METHODS
Photochemical reaction was utilized to induce localized microcirculation damage to the second cochlear turn. After systemic administration of rose bengal, the second cochlear turn was illuminated with green light (wave length 540 nm +/- 40 nm) to cause thrombosis in the stria vascularis, the area was about 1.2 x 1.0 mm2.


RESULTS
At the site of the illumination, cochlear blood flow gradually decreased, and the threshold of action potential gradually increased. Surface preparing showed thrombus was in the stria vascularis. Three hours after the photochemical reaction, some hair cells in the illuminated area necrotized. The length of the lesion was 1152.50 +/- 363.26 microns (n = 4). But hair cells in the non-illuminated area were normal.


CONCLUSIONS
The photochemical method is a reliable tool to produce an animal model with the restricted microvessel lesion in the lateral wall of the cochlea. It could be used as an animal model for the treatment of the cochlear thrombosis.
Department of Otolaryngology-Head and Neck Surgery, Peking University First Hospital, Beijing 100034.
Zhen
Z
Zhong
Xuepei
X
Li
Hongtao
H
Wu
Changsong
C
Wu
D000818:Animals; D003051:Cochlea / Q000098:blood supply*; D015834:Cochlear Diseases / Q000473:pathology / Q000503:physiopathology; D004195:Disease Models, Animal; D006168:Guinea Pigs; D007511:Ischemia / Q000503:physiopathology*; D008297:Male; D008833:Microcirculation; D010777:Photochemistry