The cagA status of Helicobacter pylori isolates from dyspeptic children in Turkey.
There are inconsistent reports regarding cytotoxin-associated gene A (cagA) status of Helicobacter pylori isolates and the severity of the mucosal lesions in children. The aim of this study was to determine the prevalence of cagA(+) strains and to evaluate its correlation with clinic and endoscopic findings. We examined 45 H. pylori strains that were grown on brain-heart infusion agar supplemented with 7% horse blood. Following 72 h of incubation colonies were harvested and bacterial DNA was extracted. Polymerase chain reaction primers F1 and B1 were used to amplify a 348-bp internal fragment of cagA. The prevalence of cagA in Turkish pediatric patients was 55.6%. No association was found between cagA status and the severity of gastro-duodenal lesions.
Department of Pediatrics, Section of Gastroenterology, Hacettepe University, Faculty of Medicine, 06100 Ankara, Turkey. isaltik@hacettepe.edu.tr
Inci Nur
IN
Saltik
Hülya
H
Demir
Doruk
D
Engin
Ozge Darka
OD
Ertunç
Yakut
Y
Akyön
Nurten
N
Koçak
D000293:Adolescent; D000942:Antigens, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001706:Biopsy; D002648:Child; D004269:DNA, Bacterial / Q000032:analysis; D004415:Dyspepsia / Q000453:epidemiology / Q000382:microbiology* / Q000503:physiopathology*; D005260:Female; D016481:Helicobacter Infections / Q000453:epidemiology / Q000382:microbiology / Q000503:physiopathology; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D015995:Prevalence; D011706:Pyloric Antrum / Q000473:pathology; D012720:Severity of Illness Index; D014421:Turkey / Q000453:epidemiology
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004269:DNA, Bacterial; C080866:cagA protein, Helicobacter pylori