Electrophysiological analysis of neuronal chemokine receptors.
Several studies have shown that neurons in the central and peripheral nervous systems express a variety of chemokine receptors (CKRs). Activation of these receptors can influence neuronal signaling by regulating synaptic transmission and neuronal excitability. This article presents electrophysiological methods that are currently used to study the normal and pathophysiological role for CKRs in the nervous system. Conventional electrophysiological methods such as patch-clamp recording of isolated neurons, brain slices, and heterologous expression systems are described. In addition, single-cell reverse transcription-polymerase chain reaction is discussed as a technique that can be used in conjunction with patch-clamp recording to further investigate the molecular basis of neuronal CKR activation.
Oh
Seog Bae
SB
Department of Molecular Pharmacology and Biology Chemistry, Northwestern University, 303 E. Chicago Avenue, Chicago, IL 60611, USA.
Cho
Charlene
C
Miller
Richard J
RJ
D000818:Animals; D001921:Brain / Q000378:metabolism; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D004594:Electrophysiology / Q000379:methods*; D019204:GTP-Binding Proteins / Q000378:metabolism; D006801:Humans; D009474:Neurons / Q000378:metabolism*; D018408:Patch-Clamp Techniques / Q000379:methods; D019707:Receptors, Chemokine / Q000096:biosynthesis / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D015398:Signal Transduction; D013116:Spinal Cord / Q000378:metabolism; D013997:Time Factors
D019707:Receptors, Chemokine; D019204:GTP-Binding Proteins