Protective role of certain chemicals against UV-B-induced damage in the nitrogen-fixing cyanobacterium, Nostoc muscorum.
The protective effects of L-cysteine, ascorbic acid, reduced glutathione, L-tryptophan, and sodium pyruvate against UV-B-induced damages were studied in the nitrogen-fixing cyanobacterium, Nostoc muscorum. When added to the culture suspension during UV-B treatment, these chemicals caused a significant protective effect on survival and growth of the organism. Sodium pyruvate conferred the strongest protection whereas the weakest effect was elicited by tryptophan. A 20 min exposure of a culture suspension to UV-B completely inactivated nitrogenase activity but the inactivation was strongly prevented by exogenous addition of ascorbic acid or reduced glutathione during UV-B exposure, and weakly prevented by pyruvate, cysteine and tryptophan. In vivo nitrate reductase activity was not completely lost even after 80 min of UV-B exposure, and addition of the test chemicals did not confer any significant protection to this enzyme. Whereas (14)CO(2) uptake was drastically inhibited (78% inhibition) by 30 min exposure to UV-B in the absence of any test chemical, about 76% activity remained when the UV-B exposure was given to cultures in the presence of ascorbic acid. These results suggest that the damaging effects of UV-B are substantially minimized by certain reducing agents, the protective effect being particularly strong on the O(2) sensitive enzyme, nitrogenase. Presence of these chemicals in their natural habitat or inside the cells of living organisms may partially protect/repair the damaging effects of UV-B radiation.
Division of Microbiology, IARI, New Delhi, India.
R
R
Tyagi
A
A
Kumar
M B
MB
Tyagi
P N
PN
Jha
H D
HD
Kumar
R P
RP
Sinha
D-P
DP
Häder
D001205:Ascorbic Acid / Q000494:pharmacology; D000458:Cyanobacteria / Q000378:metabolism / Q000528:radiation effects*; D003545:Cysteine / Q000494:pharmacology; D005978:Glutathione / Q000494:pharmacology; D009586:Nitrogen Fixation; D019289:Pyruvic Acid / Q000494:pharmacology; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D014364:Tryptophan / Q000494:pharmacology; D014466:Ultraviolet Rays
D011837:Radiation-Protective Agents; D019289:Pyruvic Acid; D014364:Tryptophan; D005978:Glutathione; D003545:Cysteine; D001205:Ascorbic Acid