Chloramphenicol resistance transposable element TnSs1 of Streptococcus suis, a transposon flanked by IS6-family elements.
A new transposon, designated TnSs1, which contains a chloramphenicol acetyltransferase gene flanked by direct repeats of an IS6-family element was found in a field isolate of Streptococcus suis. Polymerase chain reaction and hybridization analyses indicated that another field isolate carried the same transposon in a different location on the chromosome. A transposition assay done with a thermosensitive suicide vector showed that, among the seven TnSs1 mutants tested in this study, six formed a cointegrate between the S. suis genome and the vector with the generation of the third copy of the insertion sequence element, and one harbored one copy of TnSs1 on the chromosome as a result of a subsequent resolution step. On transposition, TnSs1 duplicated an 8-bp sequence at the target site.
Molecular Bacteriology Section, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
Daisuke
D
Takamatsu
Makoto
M
Osaki
Tsutomu
T
Sekizaki
D002702:Chloramphenicol Resistance / Q000235:genetics*; D003001:Cloning, Molecular; D004251:DNA Transposable Elements / Q000235:genetics*; D021901:DNA, Intergenic; D023061:Gene Order; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D017011:Streptococcus suis / Q000235:genetics* / Q000502:physiology
D004251:DNA Transposable Elements; D021901:DNA, Intergenic