Chromosome 8 aneuploidy in acquired cholesteatoma.
OBJECTIVE
To investigate the incidence of chromosome 8 aneuploidy in acquired cholesteatoma.


METHODS
Cholesteatoma tissue and postauricular skin as a control were surgically obtained from 12 patients with acquired cholesteatoma. Fluorescence in situ hybridization (FISH) analysis using a chromosome 8-specific alpha-satellite DNA probe was performed on the interphase nuclei. Two hundred cells were analyzed for each of the samples.


RESULTS
Chromosome 8 aneuploidy was found in 9/12 patients whereas a normal cell structure with 2 signals was observed in the remaining 3 patients. In samples with chromosome 8 aneuploidy, the mean proportion of aneuploidy was 25.6%, including monosomy (3.2%), trisomy (16.1%), tetrasomy (4.9%) and more than tetrasomy (1.4%). The number of aneuploid cells in recurrent cases was more than that in non-recurrent cases.


CONCLUSIONS
A numerical aberration of chromosome 8 was found in patients with acquired cholesteatoma. Our results support the hypothesis that chromosome 8 may be a prognostic factor for cholesteatoma and an indicator in the follow-up of patients with cholesteatoma.
Yildirim
Mahmut Selman
MS
Department of Genetics , Faculty of Medicine, Selcuk University, Konya, Turkey. mselman@dostmail.com
Ozturk
Kayhan
K
Acar
Hasan
H
Arbag
Hamdi
H
Ulku
Cagatay Han
CH
D000328:Adult; D000782:Aneuploidy; D018424:Cholesteatoma, Middle Ear / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D002898:Chromosomes, Human, Pair 8; D046508:Culture Techniques; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008416:Mastoid / Q000473:pathology; D008875:Middle Aged; D012008:Recurrence