A novel thiocationic liposomal formulation of antisense oligonucleotides with activity against Mycobacterium tuberculosis.
This study describes the development of a novel thiocationic (OBEHYTOP) lipid-based formulation of phosphorothioate antisense oligonucleotides (PAOs) showing inhibitory activity against mycobacterium tuberculosis (mTB) as measured by an in vitro BACTEC 460TB assay. PAOs were designed based on sequences complementary to essential regions of the mycobacterial genome from published nucleic acid databases in GenBank. These included the superoxide dismutase sod A gene (TBS3), catalase-peroxidase katG gene (TBK1, TBK10), RNA polymerase beta-subunit rpo B gene (TBR5) and diaminopimelate decarboxylase lys A gene (TBL5). The effect of PAOs (TBS3, K1, K10, R5 and L5) alone on mTB was not significant compared with the no-drug control over a period of exposure of 150 h (ranges of -11.8 to +23.58% at 72 h; 15.26 to +25.82% at 96 h and -5.51 to +24.00% at 150 h). Liposomal formulations (10:5:2 OBEHYTOP:oleic acid:vitamin D3) of PAOs resulted in statistically significant (p < 0.05 in all cases) inhibition (ranges of -51.45 to -63.00% at 72 h; -56.75 to -67.96% at 96 h; -51.45 to -60.26% at 150 h) compared with PAOs alone, thiocationic liposomal control and liposomal components. Positive controls of streptomycin and isoniazid used at their minimum inhibitory concentrations of 2.00 and 0.10 microM, respectively, resulted in average % inhibition values of -94% and -97.36%, respectively, indicating that these thiocationic lipid-formulated PAOs showed inhibitory activity directed against mTB in vitro.
Das
Aditya R
AR
Inhale Therapeutic Systems, San Carlos, California 94070, USA. adas@inhale.com
Dattagupta
Nanibhushan
N
Sridhar
C N
CN
Wu
Whei-Kuo
WK
D000704:Analysis of Variance; D000818:Animals; D000995:Antitubercular Agents / Q000494:pharmacology*; D001483:Base Sequence; D002626:Chemistry, Pharmaceutical; D016503:Drug Delivery Systems; D004352:Drug Resistance, Microbial; D007538:Isoniazid / Q000494:pharmacology*; D008081:Liposomes; D051379:Mice; D008807:Mice, Inbred BALB C; D008826:Microbial Sensitivity Tests; D023421:Models, Animal; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000254:growth & development; D016376:Oligonucleotides, Antisense / Q000737:chemistry / Q000494:pharmacology*; D010597:Pharmacogenetics; D016133:Polymerase Chain Reaction; D011336:Probability; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D018088:Tuberculosis, Multidrug-Resistant / Q000188:drug therapy / Q000517:prevention & control
D000995:Antitubercular Agents; D008081:Liposomes; D016376:Oligonucleotides, Antisense; D011933:Reagent Kits, Diagnostic; D007538:Isoniazid