Genotoxicity of hydrated sulfur dioxide on root tips of Allium sativum and Vicia faba.
Genotoxicity of sulfur dioxide (SO(2)) and its hydrates (bisulfite and sulfite) in human lymphocytes and other mammalian cells have been found earlier in our laboratory. In the present studies, we used Allium stavium and Vicia faba cytogenetic tests, which are the highly sensitive and simple plant bioassays. A mixture of sodium bisulfite and sodium sulfite (1:3), at various concentrations from 1 x 10(-4) to 2 x 10(-3)M was used for the treatment. Genotoxicity was expressed in terms of anaphase aberration (AA) frequencies in the Vicia-AA test and in terms of micronuclei (MCN) frequencies in both Vicia-MCN test and Alllium-MCN test. On average, the results showed a 1.7-3.9-fold increase of AA frequencies and a 3.5-4.5-fold increase of MCN frequencies in Vicia root tips as compared with the negative control. Similarly, results of Allium-MCN test also showed a significant increase in MCN frequencies in the treated samples. In addition, pycnotic cells (PNC) appeared in Allium root tips of treated groups. The frequencies of MCN, AA and PNC increased dose-dependently and the cell cycle delayed at the same time in bisulfite treated samples. Results of the present study suggest that the Vicia and Allium cytogenetic bioassays are efficient, simple and reproducible in genotoxicity studies of bisulfite.
Yi
Huilan
H
Institute of Environmental Medicine and Toxicology, School of Life Sciences and Technology, Shanxi University, 030006, Taiyuan, China.
Meng
Ziqiang
Z
D000393:Air Pollutants; D000705:Anaphase / Q000187:drug effects; D000975:Antioxidants; D001681:Biological Assay; D005737:Garlic / Q000187:drug effects*; D007700:Kinetics; D048629:Micronuclei, Chromosome-Defective / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects*; D013447:Sulfites / Q000494:pharmacology; D013458:Sulfur Dioxide / Q000737:chemistry*; D013997:Time Factors; D031307:Vicia faba / Q000187:drug effects*; D014867:Water / Q000737:chemistry
D000393:Air Pollutants; D000975:Antioxidants; D013447:Sulfites; D014867:Water; D013458:Sulfur Dioxide; C009279:sodium bisulfite; C025026:sodium sulfite