Matrix metalloproteinases-2 and -9 are secreted from human fibroblasts.
To investigate the pathophysiological role of fibroblasts in tissue metabolism, we analysed gelatin-degrading enzymes secreted from skin cells in culture. Using a gelatin-zymography method, matrix metalloproteinase (MMP)-2 was constitutively secreted from fibroblasts, whereas both MMP-2 and MMP-9 were secreted from keratinocytes. MMP-9 expression by fibroblasts was induced by the addition of transforming growth factor (TGF)-beta1 or tumour necrosis factor (TNF)-alpha to the cultures. Moreover, TGF-beta1, TNF-alpha, or epidermal growth factor augmented the secretion of MMP-2 from fibroblasts. These results indicate that MMP-9 together with MMP-2 secreted from fibroblasts could play important roles in tissue metabolism, including cytokine-induced inflammation.
Kobayashi
Takashi
T
Department of Dermatology/Clinical Biology of Extracellular Matrix (F7), Chiba University School of Medicine, Chiba, Japan. tkobayas@derma01.m.chiba-u.ac.jp
Hattori
Shunji
S
Shinkai
Hiroshi
H
D018929:Cell Culture Techniques; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D012867:Skin / Q000187:drug effects / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha; D004815:Epidermal Growth Factor; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9