Hepatoprotective and free radical scavenging effects of Nelumbo nucifera.
Ethanol extracts from Nelumbo nucifera (ENN) seeds were studied for possible antioxidative and hepatoprotective effects. Antioxidative effects were measured spectrophotometrically by reduction of 2,2'-Diphenyl-1-picrylhydrazyl (DPPH) radicals. Hepatoprotective effects were tested using carbon tetrachloride (CCl4) and aflatoxin B1 (AFB1)-induced hepatocyte toxicity models. ENN showed potent free radical scavenging effects with a median inhibition concentration of 6.49 microg/ml. Treatment of hepatocytes with ENN inhibited both the production of serum enzymes and cytotoxicity by CCl4. The genotoxic and cytotoxic effects of AFB1 were also inhibited by ENN in dose-dependent manners. These hepatoprotective effects of ENN against CCl4 and AFB1 might result from its potent antioxidative properties.
Sohn
D H
DH
College of Pharmacy and Medicinal Resources Research Center, Wonkwang University, Iksan, Jeonbuk, Korea.
Kim
Y C
YC
Oh
S H
SH
Park
E J
EJ
Li
X
X
Lee
B H
BH
D016604:Aflatoxin B1 / Q000633:toxicity; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001713:Biphenyl Compounds; D002251:Carbon Tetrachloride / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000494:pharmacology*; D022781:Hepatocytes / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000293:injuries; D008297:Male; D031653:Nelumbo; D008517:Phytotherapy; D010851:Picrates / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D011916:Rats, Inbred F344; D012639:Seeds / Q000737:chemistry
D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D010936:Plant Extracts; D016604:Aflatoxin B1; D002251:Carbon Tetrachloride; C004931:1,1-diphenyl-2-picrylhydrazyl