Distinct T cell subsets and cytokine production in cultures derived from transformation zone and squamous intraepithelial lesion biopsies of the uterine cervix.
OBJECTIVE
The characterization of lymphocytes issued from squamous intraepithelial lesions (SIL) and from the transformation zone (TZ), where the majority of SIL occur, is important to understand the role of immunity in SIL development.


METHODS
We compared lymphocyte populations of the TZ and SIL with those of normal exocervix, using a technique allowing for the isolation of lymphocytes, either from the epithelium or from the underlying stroma of small biopsies.


RESULTS
The majority of cells derived from the epithelium of all biopsies were CD8+ T cells. Some SIL-derived cultures were characterized by an increased proportion of activated TCRgammadelta+. The production of the immunosuppressive cytokine IL10 was significantly higher in lymphocyte cultures from the normal TZ in comparison with the exocervix. A decreased percentage of effector T cells was observed in cultures derived from the stroma of normal TZ (TCRgammadelta+) or SIL (CD8+) in comparison with the exocervix.


CONCLUSIONS
Our results suggest that a low proportion of effector T cells and IL10 production could contribute to the predisposition of the TZ to the development of SIL and to the progression of SIL to cervical cancer.
Jacobs
Nathalie
N
Department of Pathology, University of Liège, Liège, Belgium. n.jacobs@ulg.ac.be
Renard
Isabella
I
Al-Saleh
Walid
W
Hubert
Pascale
P
Doyen
Jean
J
Kedzia
Witold
W
Boniver
Jacques
J
Delvenne
Philippe
P
D001706:Biopsy; D002471:Cell Transformation, Neoplastic / Q000378:metabolism; D002584:Cervix Uteri / Q000166:cytology / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D005260:Female; D006801:Humans; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D015375:Receptors, Interleukin-2 / Q000378:metabolism; D017154:Stromal Cells / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000276:immunology / Q000473:pathology*; D002578:Uterine Cervical Dysplasia / Q000276:immunology / Q000473:pathology*
D016207:Cytokines; D011948:Receptors, Antigen, T-Cell; D015375:Receptors, Interleukin-2