A chemotaxonomic method to quantify phytoplankton groups in freshwater lentic mesocosms: an approach including chlorophyll a breakdown products.
The use of HPLC methods to determine and quantify phytoplankton population composition, is sometimes less time-consuming than microscopic identification. However, its general application poses problems since high discrepancies between chlorophyll a calculated using chemotaxonomic methods and direct measurements were noticed. For instance, chemotaxonomic protocols generally employed can lead to a poor estimation of total and relative abundance when high amounts of chlorophyll a breakdown products are present. Therefore, we propose a new approach to calculate relative abundance of algal groups in a phytoplankton population, based on integration of these degradation products in the chemotaxonomic assessment in lentic and shallow freshwater ecosystems.
Deydier-Stephan
Laurence
L
UMR 6553 ECOBIO, université de Rennes-1, 35042 Rennes, France. Laurence.Deydier-Stephan@univ-rennes1.fr
Bertru
Georges
G
Le Rouzic
Bertrand
B
D002734:Chlorophyll / Q000378:metabolism; D000077194:Chlorophyll A; D002735:Chlorophyllides / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002965:Classification / Q000379:methods; D017753:Ecosystem; D056890:Eukaryota / Q000145:classification* / Q000378:metabolism; D005602:France; D005618:Fresh Water; D010839:Phytoplankton / Q000145:classification* / Q000378:metabolism; D012621:Seasons
D002735:Chlorophyllides; D002734:Chlorophyll; C034388:chlorophyllide a; D000077194:Chlorophyll A