Phenylboronate-chitosan resins for adsorption of beta-amylase from soybean extracts.
Isolation and purification of bioproducts from crude extracts can be obtained by affinity methods based on reversible binding of a specific molecule to ligand immobilized in a porous matrix. In the present work, nicrospheres based on chitosan matrix, which incorporated aminophenylboronic acid as a derivative, were prepared and characterized, aimed at developing a beta-amylase adsorption process. Kinetic curves and adsorption isotheriru of the crude extracts as well as the breakthrough curves for a frontal chromatographic separation method of a commercial sample of beta-amylase from soybean are presented. These results were compared to similar data obtained with a comercial microspheres gel based-on agarose.
Pharmacy and Biochemistry, Dom Bosco Catholic University, UCDB, C.P. 100, CEP 79117-900, Campo Grande, MS, Brazil.
Eduardo J
EJ
Arruda
Cesar C
CC
Santana
D000327:Adsorption; D001897:Boronic Acids; D002686:Chitin / Q000031:analogs & derivatives*; D048271:Chitosan; D003188:Composite Resins / Q000138:chemical synthesis*; D007700:Kinetics; D013025:Glycine max / Q000201:enzymology*; D001614:beta-Amylase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
D001897:Boronic Acids; D003188:Composite Resins; D002686:Chitin; D048271:Chitosan; D001614:beta-Amylase; C010686:benzeneboronic acid