[Effect of high glucose condition on the secretion of TGF beta by cultured macrovascular endothelial cells of the diabetic rat].
OBJECTIVE
This study was designed to examine the secretion of TGF-beta by cultured rat thoracic aortic endothelial cells of the diabetic rats under high gluocose condition.


METHODS
First we created diabetic rat models, then the thoracic aortic endothelial cells of the diabetic rats were isolated and cultured in 10% FBS-RPMI 1640 media with various high glucose concentrations (12.5 mmol/L, 25 mmol/L or 50 mmol/L D-glucose), high glucose and TNF-alpha or high glucose and insulin for 48 hours, and the supernatant obtained from the cultured aortic endothelial cells was collected. The level of TGF-beta 1 in the supernatant was measured using a standard bioassay, which utilizes the TGF-beta 1 sensitive Mv1Lu (CLL-64) Mink lung epithelial cell line, and the production of endothelin (ET) in the supernatant was also assessed by RIA (radio immol/Lunoassay).


RESULTS
Both total TGF-beta 1 (406 +/- 29 vs 189 +/- 23 pg/ml, P < 0.05) and active TGF-beta 1 (161 +/- 24 vs 39.5 +/- 8 pg/ml) secreted by the cultured aortic endothelial cells under high glucose condition (25 mmol/L) were significantly increased as compared with control. TNF-alpha could enhance the effect of high glucose on the secretion of TGF-beta 1, whereas insulin could inhibit it. With various high glucose concentrations, the levels of ET were increased. Also TNF-alpha enhanced it and insulin inhibited it.


CONCLUSIONS
TGF-beta 1 may be involved in the development of diabetic macrovascular complication.
Department of Internal Medicine, First Affiliated Hospital, WCUMS, Chengdu 610041, China.
Y
Y
Tao
X
X
Liu
O
O
Qiang
D000818:Animals; D001011:Aorta / Q000473:pathology; D002478:Cells, Cultured; D003470:Culture Media; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000473:pathology; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005947:Glucose / Q000494:pharmacology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D003470:Culture Media; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D005947:Glucose