Overexpression of the immunoglobulin superfamily members CDO and BOC enhances differentiation of the human rhabdomyosarcoma cell line RD.
Rhabdomyosarcoma is a childhood tumor of the skeletal muscle lineage in which cells display defects in both biochemical and morphological aspects of differentiation. The immunoglobulin superfamily members CDO and BOC are components of a cell surface receptor that positively regulates myogenesis in vitro. Expression of Cdo and Boc in myoblast cell lines is downregulated by the ras oncogene, and forced re-expression of either Cdo or Boc can override ras-induced inhibition of myogenic differentiation [Kang et al., J Cell Biol 1998; 143:403-413; Kang et al., EMBO J 2002; 21:114-124]. The current study sought to test whether the promyogenic properties of CDO and BOC could be extended to a human rhabdomyosarcoma cell line, RD. Stable overexpression of CDO or BOC in RD cells led to enhanced expression of two markers of muscle cell differentiation, troponin T and myosin heavy chain, and to increased formation of elongated, myosin heavy chain-positive myotubes. These observations are consistent with the notion that CDO and BOC play a role in the inverse relationship between differentiation and transformation of cells in the skeletal muscle lineage.
Wegorzewska
Marta
M
Brookdale Department of Molecular, Cell and Developmental Biology, Mount Sinai School of Medicine, New York, New York 10029, USA.
Krauss
Robert S
RS
Kang
Jong-Sun
JS
D015153:Blotting, Western; D015815:Cell Adhesion Molecules; D002454:Cell Differentiation; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism*; D024510:Muscle Development; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012208:Rhabdomyosarcoma / Q000378:metabolism* / Q000473:pathology*; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins
C474334:BOC protein, human; C107286:CDON protein, human; D015815:Cell Adhesion Molecules; D007074:Immunoglobulin G; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D025521:Tumor Suppressor Proteins