[Clinical evaluation of performance of a new diagnostic method for deep mycosis by measuring beta-glucan concentration in the blood].
beta-Glucan Test MARUHA for high sensitive diagnosis of deep mycosis which was developed recently detects (1-->3)-beta-D-glucan, a component of the cell wall. The performance of beta-Glucan Test MARUHA is evaluated in this report. Although existing methods to detect (1-->3)-beta-D-glucan have trouble with sulfa drugs, hemolysis, and immunoglobulin G (IgG), these problems were overcome by the beta-Glucan Test MARUHA: No effect was observed for beta-Glucan Test MARUHA at lower than 200 micrograms/ml, 500 mg/dl, and 6,000 mg/dl of sulfa drugs, hemoglobin, and IgG, respectively. The effect of drugs administrated on the measurement value of beta-glucan Test MARUHA was checked at several concentrations. However, almost no effect of drugs, such as, 5 kinds of antifungals, 8 kinds of antibiotics, a kind of antibacterial, 2 kinds of infusions, and a kind of contrast media was observed at the practical concentrations.
Moro
Hiroshi
H
Division of Clinical Infection Control and Prevention, Niigata University Graduate School of Medical and Dental Sciences.
Tsukada
Hiroki
H
Ohara
Tatsuki
T
Susa
Ritsuko
R
Tanabe
Yoshinari
Y
Suzuki
Eiichi
E
Gejyo
Fumitake
F
D015415:Biomarkers / Q000097:blood; D005069:Evaluation Studies as Topic; D005936:Glucans / Q000097:blood*; D006801:Humans; D009181:Mycoses / Q000175:diagnosis*; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D012680:Sensitivity and Specificity; D047071:beta-Glucans
D015415:Biomarkers; D005936:Glucans; D011933:Reagent Kits, Diagnostic; D047071:beta-Glucans; C033363:beta-1,3-glucan