Characterization of rice functional monosaccharide transporter, OsMST5.
cDNA of a monosaccharide transporter in rice, OsMST5 (Oryza sativa monosaccharide transporter 5) was cloned and its sugar transport activity was characterized by heterologous expression analysis. The amino acid sequence and topology were similar to the sequences and topology of other plant monosaccharide transporters. Yeast cells co-expressed with OsMST5 cDNA transported some monosaccharide substrates. The transport rate increased when ethanol as an electron donor was added, so the transporter was an energy-dependent active one. Most of the OsMST5 was expressed in panicles before pollination, indicating that it is associated with pollen development in rice.
Graduate School of Science, Hokkaido University, Kita-ku N10-W8, Sapporo 060-0810, Japan.
Budsaraporn
B
Ngampanya
Anna
A
Sobolewska
Taito
T
Takeda
Kyoko
K
Toyofuku
Jarunya
J
Narangajavana
Akira
A
Ikeda
Junji
J
Yamaguchi
D000595:Amino Acid Sequence; D001692:Biological Transport; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004734:Energy Metabolism; D000431:Ethanol / Q000494:pharmacology; D006601:Hexoses / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D012275:Oryza / Q000235:genetics* / Q000378:metabolism*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D015003:Yeasts / Q000378:metabolism
D018076:DNA, Complementary; D006601:Hexoses; D008565:Membrane Proteins; D009004:Monosaccharide Transport Proteins; D000431:Ethanol; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone