Biosorption of cadmium, copper, lead and zinc by inactive biomass of Pseudomonas Putida.
The accumulation of Cd(II), Cu(II), Pb(II) and Zn(II) at mg L(-1) concentration levels by inactive freeze-dried biomass of Pseudomonas Putida has been investigated. These metals could be efficiently removed from diluted aqueous solutions. A contact time of 10 min was sufficient to reach equilibrium. The pH has a strong effect on metal biosorption and the optimal pH values were 6.0, 5.0-6.0, 6.0-6.5 and 7.0-7.5 for Cd(II), Cu(II), Pb(II) and Zn(II) respectively. Under these conditions there was 80% removal for all metals studied. The process of biosorption can be described by a Langmuir-type adsorption model. This model accounts for 98% of the data variance. The K(A) and q(max) parameters for each metal are strongly correlated (at confidence levels greater than 98%) with the metal acidity, quantified by the constant of the corresponding M(OH)(+) complex, thus confirming previous assertions by other authors.
Pardo
Rafael
R
Departamento de Química Analítica, Universidad de Valladolid, Facultad de Ciencias, Prado de la Magdalena, Spain. rpardo@qa.uva.es
Herguedas
Mar
M
Barrado
Enrique
E
Vega
Marisol
M
D000327:Adsorption; D018533:Biomass; D002104:Cadmium / Q000378:metabolism*; D003300:Copper / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007854:Lead / Q000378:metabolism*; D008433:Mathematics; D016958:Pseudomonas putida / Q000378:metabolism*; D013997:Time Factors; D015032:Zinc / Q000378:metabolism*
D002104:Cadmium; D007854:Lead; D003300:Copper; D015032:Zinc