Fumonisin-induced blockade of ceramide synthase in sphingolipid biosynthetic pathway alters aortic input impedance spectrum of pigs.
The sphingolipid signaling pathway appears to play an important role in regulating vascular tone. We examined the effect of fumonisin B(1), a fungal toxin in corn that blocks ceramide synthase in the sphingolipid signaling pathway, on the ascending aortic impedance spectrum of pigs. Sixteen pigs were fed culture material containing fumonisin B(1) (20 mg/kg body wt) (n = 7) or a control diet (n = 9) daily for 3 days and then instrumented under alpha-chloralose anesthesia for measurement of ascending aortic pressure and flow. Fumonisin ingestion increased serum sphinganine and sphingosine concentrations. Fumonisin ingestion also decreased cardiac output and characteristic impedance and increased the frequency of the first minimum impedance modulus, systemic vascular resistance, and the terminal, first, and second harmonic reflection coefficients, without changing mean arterial pressure. Thus blockade of ceramide synthase is accompanied by decreased vascular tone in systemic conduit arteries and increased vascular tone in systemic resistance vessels. The results indicate that the sphingolipid signaling pathway influences vascular tone in alpha-chloralose-anesthetized pigs.
Department of Veterinary Clinical Medicine, University of Illinois at Urbana-Champaign, Illinois 61802, USA. p-constable@uiuc.edu
Peter D
PD
Constable
Geoffrey W
GW
Smith
George E
GE
Rottinghaus
Mike E
ME
Tumbleson
Wanda M
WM
Haschek
D000217:Acyltransferases / Q000037:antagonists & inhibitors*; D000316:Adrenergic alpha-Agonists / Q000494:pharmacology; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000502:physiology; D001784:Blood Gas Analysis; D002118:Calcium / Q000494:pharmacology; D002138:Calibration; D017097:Electric Impedance; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D037341:Fumonisins / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects / Q000502:physiology*; D007545:Isoproterenol / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D009599:Nitroprusside / Q000494:pharmacology; D010656:Phenylephrine / Q000494:pharmacology; D018343:Receptors, Adrenergic, beta-2 / Q000187:drug effects / Q000502:physiology; D015398:Signal Transduction / Q000187:drug effects; D013107:Sphingolipids / Q000096:biosynthesis*; D051049:Sphingosine N-Acyltransferase; D013552:Swine; D014655:Vascular Resistance / Q000502:physiology; D014664:Vasodilation / Q000187:drug effects; D014665:Vasodilator Agents / Q000494:pharmacology
D000316:Adrenergic alpha-Agonists; D000318:Adrenergic beta-Agonists; D004791:Enzyme Inhibitors; D037341:Fumonisins; D018343:Receptors, Adrenergic, beta-2; D013107:Sphingolipids; D014665:Vasodilator Agents; D009599:Nitroprusside; D010656:Phenylephrine; D000217:Acyltransferases; D051049:Sphingosine N-Acyltransferase; D007545:Isoproterenol; D002118:Calcium