A combinatorial approach to obtain affinity media with binding properties towards the aflatoxins.
In our work we performed a combinatorial solid-phase synthesis in aqueous medium to prepare peptide libraries from which to select an amino acid sequence with binding properties towards aflatoxins. We used polystyrene beads, functionalised with carboxylic groups as solid support and eight amino acids as monomers. During the first step 64 different sequences of two amino acids were prepared by exploiting the principles of combinatorial chemistry; then the binding properties of all sequences towards aflatoxin B(1 )were checked. We determined binding constants towards aflatoxin B(1) and towards aflatoxins B(2), G(1) and G(2). Results were promising, so we prepared a new library by using the selected dipeptide as the starting solid phase. After selecting the best tetrapeptide sequences, we determined binding constants towards the quoted aflatoxins. We obtained binding constants ( K>10(4) M(-1)) similar to those shown by human serum albumin for similar compounds. Preliminary studies on an extraction column were promising for the development of an SPE system and for its application in food matrices.
Tozzi
Cinzia
C
Department of Analytical Chemistry, University of Turin, Via P. Giuria, 5-10125, Turin, Italy. cinzia.tozzi@unito.it
Anfossi
Laura
L
Baggiani
Claudio
C
Giovannoli
Cristina
C
Giraudi
Gianfranco
G
D016604:Aflatoxin B1 / Q000032:analysis / Q000378:metabolism; D000348:Aflatoxins / Q000032:analysis* / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism; D002846:Chromatography, Affinity / Q000295:instrumentation*; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D011485:Protein Binding; D013816:Thermodynamics
D000348:Aflatoxins; D000596:Amino Acids; C027955:aflatoxin G1; C029754:aflatoxin G2; C029753:aflatoxin B2; D016604:Aflatoxin B1