Ozone treatment for reduction of Escherichia coli 0157:H7 and Salmonella serotype typhimurium on beef carcass surfaces.
The effectiveness of an aqueous ozone treatment in reducing Escherichia coli O157:H7 and Salmonella serotype Typhimurium on hot carcass surfaces was determined with the use of a model carcass spray cabinet. Carcass surface regions were removed from carcasses and inoculated with feces containing 10(6) to 10(7) CFU each of E. coli O157:H7 and Salmonella Typhimurium per g and were then exposed to a water wash or to a water wash followed by a sanitizing ozone treatment. Water washes were applied at 28 degrees C beginning at a pressure of 10 lb/in2 and gradually increasing to 400 lb/in2. Ozone treatment was carried out by spraying surfaces with an aqueous ozone solution (80 lb/in2 at 28 degrees C) containing 95 mg of ozone per liter. Pathogen reductions achieved with ozone treatment were not significantly different from those achieved with a water wash alone. In addition, ozone treatment did not reduce E. coli O157:H7 or Salmonella Typhimurium contamination that was spread over the carcass surface as a result of the water wash. Under the conditions of this study, the aqueous ozone treatment applied resulted in no significant improvement over a water wash in reducing pathogens on beef carcass surfaces.
Castillo
A
A
Department of Animal Science, Texas A&M University, 2471 TAMU, College Station, Texas 77843-2471, USA.
McKenzie
K S
KS
Lucia
L M
LM
Acuffi
G R
GR
D000818:Animals; D002417:Cattle; D015169:Colony Count, Microbial; D004203:Disinfection / Q000379:methods*; D019453:Escherichia coli O157 / Q000187:drug effects* / Q000254:growth & development; D005516:Food Microbiology; D008297:Male; D008460:Meat / Q000382:microbiology*; D010083:Oxidants, Photochemical / Q000494:pharmacology*; D010126:Ozone / Q000494:pharmacology*; D011312:Pressure; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000254:growth & development; D013696:Temperature; D016896:Treatment Outcome; D014867:Water
D010083:Oxidants, Photochemical; D014867:Water; D010126:Ozone