[Effect of sepia on intracellular Ca2+ concentration, nuclear Ca2+/Mg(2+)-ATPase activities and c-jun expression in H22 cancer cells].
The effect of sepia on the intracellular Ca2+ concentration, nuclear Ca2+/Mg(2+)-ATPase activities and the expression of c-jun were studied in H22 cancer cells by using fluorescent probe and immunohistochemical method. The results showed that intracellular Ca2+ concentration decreased 69% and 79%, nuclear Ca2+/Mg(2+)-ATPase activities diminished 21% and 37%, c-jun expression decreased obviously. The results suggested that sepia probably diminished the intracellular Ca2+ concentration, affected Ca(2+)-dependent nuclear Ca2+/Mg(2+)-ATPase activities, thus reduced the amount of Ca2+ transporting into nuclei, so lessened the promoting effect of Ca2+ on c-jun expression, and therefore inhibited cellular differentiation and proliferation. This might be one of the possible anti-cancer mechanisms of sepia.
Institute of Environmental Health Monitoring, Chinese Academy of Preventive Medicine, Beijing 100021, China.
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Wang
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Sun
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Xie
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Su
D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002467:Cell Nucleus / Q000201:enzymology / Q000378:metabolism; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007424:Intracellular Fluid / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000473:pathology; D008419:Materia Medica / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D008974:Mollusca / Q000737:chemistry; D009368:Neoplasm Transplantation; D016755:Proto-Oncogene Proteins c-jun / Q000096:biosynthesis* / Q000235:genetics; D014407:Tumor Cells, Cultured
D008419:Materia Medica; D016755:Proto-Oncogene Proteins c-jun; D017301:Ca(2+) Mg(2+)-ATPase; D002118:Calcium