[Electric capacitance of bilayer lipid membranes from hydrogenated egg lecithin during phase transition from liquid crystalline state to gel].
The electrical capacity of planar bilayer lipid membranes (BLM) from natural hydrogenated egg lecithin (HEL) in n-decane at a temperature of phase transition was measured. The temperature of phase transition was determined calorimetrically to be 51 degrees C. The data obtained revealed a phase separation of HEL in BLM into two fractions, one freezing at 42-44 degrees C and one that is converted to a liquid-crystal state at 51-59 degrees C. It was assumed that the first fraction is rich in dipalmitoyl lecithin, and the second fraction is rich in distearoyl lecithin. Freezing and the transition to the liquid-crystal state were accompanied by an increase and decrease in membrane thickness, respectively, in part due to a displacement of the solvent from the torus to the planar part of the bilayer. The displacement of the solvent is explained by changes in the disjoining pressure in BLM, which arises across the lipid bilayer due to van der Waals forces of attraction between water layers on both sides of the BLM.
Anosov
A A
AA
Sechenov Moscow Medical Academy, Bol'shaya Pirogovskaya ul. 2-6, Moscow, GSP-3, 119992 Russia.
Bogatyreva
N E
NE
Korepanova
E A
EA
Smirnova
E Iu
EIu
Norik
V P
VP
Antonov
V F
VF
D000818:Animals; D002645:Chickens; D003460:Crystallization; D004531:Eggs; D025802:Electric Capacitance; D005782:Gels; D006865:Hydrogenation; D008051:Lipid Bilayers / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry*
D005782:Gels; D008051:Lipid Bilayers; D010713:Phosphatidylcholines