The role of ascorbic acid on the redox status and the concentration of malondialdehyde in streptozotocin-induced diabetic rats.
We investigated the role of ascorbic acid on the redox status in streptozotocin-induced diabetic rats. In the plasma of diabetic rats, the ratio of reduced/total ascorbic acid was significantly decreased as compared with normal control. Ascorbic acid supplementation increased the reduced and total ascorbic acid contents as compared with diabetic control. In the rutin-treatment group, reduced and total contents of ascorbic acid were significantly decreased, however, the ratio of reduced/total contents of ascorbic acid had no difference as compared with diabetic rats. In the insulin-treatment group, this ratio is not significantly different as compared with diabetic control. However, in the insulin plus ascorbic acid treatment group, reduced form and the ratio of reduced/total ascorbic acid were significantly increased as compared with diabetic control. In addition, we measured the contents of malondialdehyde (MDA) in the plasma of diabetic rats. The contents of MDA was increased as compared with normal control, however, in insulin-treatment group, the contents of MDA was decreased as compared with diabetic rats. Ascorbic acid had no effects on the increases of MDA in diabetic rats. In conclusion, plasma ascorbic acid level and its reduced/total ratio reflects the status of the oxidative stress in the diabetic rats. Supplement of ascorbic acid did not correct the ratio of the reduced/ total ascorbic acid. However, supplement of insulin and ascorbic acid corrected the ratio of reduced/total ascorbic acid.
Department of Pharmacology, College of Pharmacy, Chung Ang University, Seoul 156-756, Korea.
Hee Jung
HJ
Choi
Hyun Dong
HD
Je
Ji Hoon
JH
Jeong
Young Sil
YS
Min
Tae Sik
TS
Choi
Joon Hong
JH
Park
Chang Yell
CY
Shin
Uy Dong
UD
Sohn
D000818:Animals; D001205:Ascorbic Acid / Q000097:blood* / Q000494:pharmacology / Q000627:therapeutic use; D003921:Diabetes Mellitus, Experimental / Q000097:blood* / Q000188:drug therapy; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley
D008315:Malondialdehyde; D001205:Ascorbic Acid