Optimal parameters for arterial repair using light-activated surgical adhesives.
The clinical acceptance of laser-tissue repair techniques is dependent on the reproducibility of viable repairs. Reproducibility is dependent on two factors: (i) the choice of materials to be used as the adhesive; and (ii) obtaining temperatures high enough to cause protein denaturation at the vital tissue interface without causing excessive thermal damage to the surrounding tissue. The use of a polymer scaffold as a carrier for the protein solder provides for uniform application of the solder to the tissue, thus allowing for pre-selection of optimal laser parameters. The scaffold also facilitates precise tissue alignment and ease of clinical application. In addition, the scaffold can be doped with various pharmaceuticals such as hemostatic and thrombogenic agents to aid wound healing. An ex vivo study was performed to correlate solder and tissue temperature with the tensile strength of arterial repairs formed using scaffold-enhanced light-activated surgical adhesives. Previous studies by our group using solid protein solder without the scaffold indicate that a solder/tissue, interface temperature of 65 degrees C is optimal. Using this parameter as a benchmark, laser irradiance was varied and temperatures were recorded at the surface and at the tissue interface of scaffold-enhanced protein solder using an infrared temperature monitoring system, designed by the researchers, and a type-K thermocouple, respectively.
Biomedical Engineering Program, Rose-Hulman Institute of Technology, Terre Haute, IN 47803, USA.
Eric C
EC
Soller
Grant T
GT
Hoffman
Karen M
KM
McNally-Heintzelman
D000818:Animals; D004307:Dose-Response Relationship, Radiation; D005263:Femoral Artery / Q000737:chemistry* / Q000293:injuries / Q000473:pathology / Q000528:radiation effects*; D066298:In Vitro Techniques; D007208:Indocyanine Green / Q000737:chemistry / Q000528:radiation effects*; D007834:Lasers; D008027:Light; D008422:Materials Testing / Q000379:methods; D011489:Protein Denaturation; D011786:Quality Control; D012680:Sensitivity and Specificity; D012709:Serum Albumin / Q000737:chemistry / Q000528:radiation effects; D013314:Stress, Mechanical; D013552:Swine; D013696:Temperature; D013718:Tensile Strength; D014014:Tissue Adhesives / Q000737:chemistry* / Q000528:radiation effects*; D014896:Welding / Q000295:instrumentation / Q000379:methods
D012709:Serum Albumin; D014014:Tissue Adhesives; D007208:Indocyanine Green