Human peritoneal macrophages in culture: a model for studying inflammatory disorders in vitro.
In this study we characterized a model of human peritoneal macrophages maintained in culture for up to 48 h that can be used to study different functions of this cell population in vitro. The cells remained viable and functionally active over time, with well-preserved phagocytic properties. They expressed a macrophage marker, CD14. Once in culture, human peritoneal macrophages secreted C1q and nitric oxide in a pattern described in murine, guinea pig, and rat peritoneal macrophages. The described model can be used to study physiology and pathophysiology of peritoneal macrophages in vitro, offering all the advantages of the use of a human cell population.
2nd Department of Medicine, Johann Wolfgang Goethe University, Theodor-Stern-Kai, 60590 Frankfurt, Germany. D.Faust@em.uni-frankfurt.de
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Faust
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Akoglu
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Faust
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Milovic
D000818:Animals; D002478:Cells, Cultured; D015922:Complement C1q / Q000276:immunology; D005434:Flow Cytometry; D006168:Guinea Pigs; D006801:Humans; D007249:Inflammation / Q000276:immunology*; D018950:Lipopolysaccharide Receptors / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000276:immunology*; D051379:Mice; D009569:Nitric Oxide / Q000276:immunology*; D051381:Rats
D018950:Lipopolysaccharide Receptors; D009569:Nitric Oxide; D015922:Complement C1q