A survey for BVDV antibodies in cattle farms in Slovakia and genetic typing of BVDV isolates from imported animals.
A serological survey for bovine viral diarrhoea virus (BVDV) antibodies on a collection of 1295 serum samples obtained from 6-12 months old cattle originating from 45 farms in Slovakia was carried out. On 13 farms more than 90% of the examined animals were seropositive, on 14 farms 71-90% seroprevalence was observed, on 13 farms only 50-70% animals were found to be positive for BVDV antibodies, while the remaining 5 farms showed fewer than 50% seropositive animals. The average incidence of BVDV antibodies (around 70%) was similar as determined 30 years ago. Of 84 serum samples from seronegative animals originating from 14 farms in which 70-98% seropositivity was observed, six were positive in Ag-BVDV ELISA indicating persistently infected (PI) cattle. On a farm to which animals were imported from abroad, a BVD outbreak was observed. Of 110 animals tested, four were positive in Ag-ELISA indicating the presence of PI cattle on this farm. Genetic typing of two isolates from imported animals performed by RT-PCR (324/326 primers from 5'-UTR), sequencing of PCR products and computer-assisted phylogenetic analysis revealed that they belong to BVDV-1 h group.
Department of Parasitology and Infectious Diseases, University of Veterinary Medicine, Komenského 73, 041 81 Kosice, Slovakia. vilcek@uvm.sk
S
S
Vilcek
Jana
J
Mojzisová
Viera
V
Bajová
S
S
Paulík
L
L
Strojný
B
B
Durkovic
Vlasta
V
Hipíková
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000453:epidemiology* / Q000276:immunology / Q000821:virology*; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D004196:Disease Outbreaks / Q000662:veterinary; D005260:Female; D008297:Male; D018154:Slovakia / Q000453:epidemiology
D000914:Antibodies, Viral