Time course of colonic nuclear factor-kappa B expression during bacterial peptidoglycan-polysaccharide-induced colitis in rats.
Nuclear factor-kappa B has been proposed to play a role in the pathogenesis of bacterial peptidoglycan-polysaccharide-induced colitis. However, its colonic expression has not been defined in detail. The primary aim of this study was to profile this expression in the rat colon. Peptigoglycan-polysaccharide was administered to the rat colon by direct intramural injections. Gross colonic injury was determined at various time points. Concomitantly, colonic nuclear factor-kappa B was measured by an electrophoretic mobility shift assay and by immunohistochemistry. Gross colonic injury and colonic nuclear factor-kappa B expression showed similar time courses following peptigoglycan-polysaccharide administration. Peak colonic injury and nuclear factor-kappa B expression were found on day 21. Nuclear factor-kappa B was mainly expressed in submucosal inflammatory cells. In conclusion, the administration of bacterial peptidoglycan-polysaccharide to the rat colon caused a chronic colitis, which was characterized by up-regulated colonic nuclear factor-kappa B.
Gastrointestinal Inflammation Department, Maryland Research Laboratories, Otsuka Maryland Research Institute, Rockville, Maryland 20850, USA.
Leo R
LR
Fitzpatrick
Jian
J
Wang
Truc
T
Le
Noel
N
Calingasan
D000818:Animals; D003092:Colitis / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D024202:Electrophoretic Mobility Shift Assay; D005260:Female; D007124:Immunoenzyme Techniques; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D016328:NF-kappa B / Q000378:metabolism*; D010457:Peptidoglycan / Q000494:pharmacology*; D051381:Rats; D011917:Rats, Inbred Lew; D013297:Streptococcus pyogenes
D016328:NF-kappa B; D010457:Peptidoglycan