CrkI adapter protein modulates cell migration and invasion in glioblastoma.
The human crk gene is translated into crkI and crkII by alternative splicing. crkII mRNA was detected both in normal brain and glioblastoma tissues, whereas crkI mRNA levels were quite low in normal brain and up-regulated in glioblastoma tissues. Expression of CrkI but not CrkII in glioblastoma U87MG cells induced transformation that stimulated cell migration and invasion concomitant with tyrosine phosphorylation of p130 Crk-associated substrate. N-cadherin-mediated signal transduction, which was essential for invasion by U87MG cells, was no longer required for CrkI-transformed cells. These results suggest that CrkI contributes to malignancy of glioblastoma by inducing phosphorylation of p130 Crk-associated substrate.
Department of Molecular Virology and Oncology, Cancer Research Institute, Graduate School of Medical Science, Kanazawa University, Japan. ttakino@kenroku.kanazawa-u.ac.jp
Takahisa
T
Takino
Mitsutoshi
M
Nakada
Hisashi
H
Miyamori
Junkoh
J
Yamashita
Kenneth M
KM
Yamada
Hiroshi
H
Sato
D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002465:Cell Movement / Q000502:physiology*; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D005909:Glioblastoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D009361:Neoplasm Invasiveness; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D050737:Proto-Oncogene Proteins c-crk; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014407:Tumor Cells, Cultured
D011518:Proto-Oncogene Proteins; D050737:Proto-Oncogene Proteins c-crk; D012333:RNA, Messenger