Selective separation of active inhibitors of epidermal growth factor receptor from Caragana jubata by molecularly imprinted solid-phase extraction.
A feasibility research was performed to study the possibilities of using a molecularly imprinted polymer as sorbent material in solid-phase extraction for the separation of active inhibitors of epidermal growth factor receptor (EGRF) from Caragana Jubata, a Chinese traditional Tibetan medicine. A molecularly imprinted polymer using quercetin, an active anti-EGFR inhibitor (IC50 = 15 microM), as the template and acrylamide as the functional monomer was prepared. The polymer was evaluated as a selective sorbent in molecularly imprinted solid-phase extraction. The EtOAc extract of Caragana Jubata was loaded on the polymer, and two novel active anti-EGFR inhibitors were found to be selectively retained after washing the polymer with appropriate solvent to disrupt the non-specific interactions occurring between the sample and the polymer matrix, which were identified as (E)-piceatannol (IC50 =4.9 microM) and butein (IC50 = 10 microM). The present work affords us a new potential method for selective separation of bioactive components from herb by using molecularly imprinted polymer as a solid-phase extraction adsorbent.
Department of Chemistry and Molecular Engineering, Yangshengtang Joint Laboratory for Natural Products, Peking University, Beijing 100871, China.
Lili
L
Zhu
Xiaojie
X
Xu
D032602:Caragana / Q000737:chemistry*; D002599:Chalcone / Q000031:analogs & derivatives* / Q000302:isolation & purification* / Q000494:pharmacology; D047188:Chalcones; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D066246:ErbB Receptors / Q000037:antagonists & inhibitors*; D006801:Humans; D011794:Quercetin / Q000302:isolation & purification* / Q000494:pharmacology; D012680:Sensitivity and Specificity; D013267:Stilbenes / Q000302:isolation & purification* / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D047188:Chalcones; D013267:Stilbenes; C040918:butein; D002599:Chalcone; C041525:3,3',4,5'-tetrahydroxystilbene; D011794:Quercetin; D066246:ErbB Receptors