Relationship between expression of Bax and Bcl-2 proteins and apoptosis in radiation compound wound healing of rats.
OBJECTIVE
To study the relationship between the expression of Bax, Bcl-2 proteins, and apoptosis in radiation compound wound healing of rats.


METHODS
Apoptosis, Bax and Bcl-2 proteins were estimated by in situ terminal labeling (TUNEL) and immunohistochemical methods.


RESULTS
(1) Changes of the apoptosis in wound healing showed three typical characteristics: early occurrence, high frequency and delayed disappearance after radiation to rats when compared with those of simple wound group, which might be an important reason for radiation-induced delayed wound healing. (2) The expression of Bax protein increased evidently with the increment of apoptosis and showed a good corresponding relationship with the apoptotic frequency in the process of wound healing. While the expression of Bcl-2 protein decreased obviously as the apoptosis reached a maximum and showed increasing tendency up to normal level when the apoptosis decreased distinctively.


CONCLUSIONS
Bax and Bcl-2 proteins play an important role in the apoptotic regulation of radiation compound wound healing in rats.
Cui
Yü-fang
YF
Department of Experimental Pathology, Institute of Radiation Medicine, Beijing 100850, China. cuiyff@yahoo.com.cn
Xia
Guo-wei
GW
Fu
Xiao-bing
XB
Yang
Hong
H
Peng
Rui-yun
RY
Zhang
Ying
Y
Gu
Qing-yang
QY
Gao
Ya-bing
YB
Cui
Xue-mei
XM
Hu
Wen-hua
WH
D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D005260:Female; D005720:Gamma Rays; D007150:Immunohistochemistry; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000473:pathology / Q000528:radiation effects; D014945:Wound Healing / Q000235:genetics / Q000528:radiation effects; D051028:bcl-2-Associated X Protein
C494828:Bax protein, rat; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein