Secondary responses of alloantigen-primed dog lymphocytes.
The usefulness of Primed Lymphocyte Typing (PLT) for recognizing Lymphocyte Defined (LD) determinants of the Major Histocompatibility Complex of the dog (DLA) was assessed in 10 separate experiments. Application of a technique described for human cell to peripheral blood lymphocytes of dogs gave reproducible, informative results. DLA LD determinants were shown to be of major importance in secondary responses of alloantigen "primed" lymphocytes, but other, a yet undefined, factors also appeared to play a role in the assay.
Lansdorpl
P M
PM
Laboratory for Experimental Surgery, Erasmus University, Rotterdam, The Netherlands.
Vriesendorp
H M
HM
Betton
G R
GR
Bijnen
A B
AB
D000818:Animals; D002478:Cells, Cultured; D004285:Dogs / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000276:immunology; D006650:Histocompatibility Testing; D007519:Isoantigens / Q000235:genetics / Q000276:immunology*; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology*; D015203:Reproducibility of Results
D015395:Histocompatibility Antigens Class I; D007519:Isoantigens; C039692:histocompatibility antigen DLA