Cloning of rat ABCA7 and its preferential expression in platelets.
We cloned the full-length cDNA of a rat orthologue of ABCA7 (rABCA7) from rat platelets. The cDNA of rABCA7 is 6510bp in length and encodes a protein of 2170 amino acids. The amino acid sequence of rABCA7 exhibits homology to those of mouse ABCA7 (92.5% identical in amino acid sequence) and human ABCA7 (76.6%). We obtained two clones of monoclonal antibodies against rABCA7 recognizing different epitopes. Analysis of CHO cells stably expressing rABCA7 by confocal laser-scanning microscopy indicated that rABCA7 is mainly located in the plasma membrane. Western blot analysis of rat tissues revealed that rABCA7 was preferentially expressed in platelets and that its apparent molecular mass was 250kDa. This is the first report of the tissue distribution of rABCA7 at the protein level and is the first reported case of ABC transporters being expressed in platelets, suggesting their important role in platelet function.
Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567-0047, Japan.
Mari
M
Sasaki
Ayako
A
Shoji
Yoshiyuki
Y
Kubo
Shigeyuki
S
Nada
Akihito
A
Yamaguchi
D018528:ATP-Binding Cassette Transporters / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism; D001792:Blood Platelets / Q000378:metabolism*; D016466:CHO Cells; D003001:Cloning, Molecular; D006224:Cricetinae; D018604:Epitope Mapping; D005260:Female; D006801:Humans; D005810:Multigene Family; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution
C411895:ABCA7 protein, human; D018528:ATP-Binding Cassette Transporters; C485856:Abca7 protein, mouse; C485857:Abca7 protein, rat; D000911:Antibodies, Monoclonal