Improved methods of isolation and purification of myxobacteria and development of fruiting body formation of two strains.
By using baiting techniques and different purification methods, a high number of myxobacterial strains have been isolated as pure cultures from soil of different regions of China. Because myxobacterial cells do not disperse easily in liquid media, a medium containing an enzymatic hydrolysate of casein (CEH) medium have been used for purification and purity tests combined in a single step. The key method, in which isolates are reintroduced to sterile rabbit dung to induce fruiting bodies formation, facilitates purification of myxobacteria. Sterile rabbit dung pellets are used to mimic the natural growth substance of these organisms which has the advantage that characteristic fruiting bodies emerge, which is a key characteristics in the taxonomy of myxobacteria. In this study, the optimum program of isolation and purification of some myxobacteria strains has been established which will facilitate screening programs. Moreover, the development of fruiting body formation of strain BD20 (Chondromyces) and strain BD54 (Cystobacter) have been recorded in this study.
Zhang
LiPing
L
College of Life Science and Branch of Key Laboratory for Microbial Resources, Ministry of Education, Hebei University, Baoding, Hebei, People's Republic of China. zhlping@sina.263.com
Wang
HaiYing
H
Fang
XiaoMei
X
Stackebrandt
Erko
E
Ding
YanBo
Y
D001431:Bacteriological Techniques; D002364:Caseins / Q000378:metabolism; D002681:China; D003470:Culture Media; D009231:Myxococcales / Q000145:classification / Q000254:growth & development* / Q000302:isolation & purification*; D012988:Soil Microbiology
D002364:Caseins; D003470:Culture Media