Calcr, a brain-specific imprinted mouse calcitonin receptor gene in the imprinted cluster of the proximal region of chromosome 6.
Expressed sequence tags (ESTs) in the human chromosome 7q21-q31 region were recently used to screen for allelic expression bias in monochromosomal hybrids retaining a paternal or maternal human chromosome 7. Six candidate imprinted genes were identified. In this study, we investigated parent-of-origin-specific expression profiles of their mouse homologues in the proximal region of chromosome 6. An imprinting analysis, using F1 mice from reciprocal crosses between the B6 and JF strains, demonstrated that the mouse calcitonin receptor gene ( Calcr) was expressed preferentially from the maternal allele in brain, whereas no allelic bias was detected in other tissues. Our results indicate that Calcr is imprinted in a tissue-specific manner, with a predominant expression from the maternal allele in the brain.
Hoshiya
Hidetoshi
H
Division of Molecular and Cell Genetics, Department of Molecular and Cellular Biology, School of Life Sciences, Faculty of Medicine, Tottori University, 86 Nishimachi, Yonago 683-8503, Japan.
Meguro
Makiko
M
Kashiwagi
Akiko
A
Okita
Chiga
C
Oshimura
Mitsuo
M
D000818:Animals; D001921:Brain / Q000378:metabolism*; D033481:Chromosomes, Mammalian / Q000235:genetics*; D020869:Gene Expression Profiling; D018392:Genomic Imprinting / Q000235:genetics*; D051379:Mice; D009928:Organ Specificity; D020161:Physical Chromosome Mapping; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018003:Receptors, Calcitonin / Q000235:genetics*
D012333:RNA, Messenger; D018003:Receptors, Calcitonin