Activated sludge exopolymers: separation and identification using size exclusion chromatography and infrared micro-spectroscopy.
Extracellular polymeric substances were extracted from activated sludge using a resin exchange method and analyzed. The separation and identification of EPS were carried out by size exclusion chromatography and Fourier transform infrared micro-spectroscopy. Chromatograms of extracted EPS exhibited seven peaks. Proteins varying in molecular weights from 670 to 45 kDa were present in all the peaks. Polysaccharides corresponding to molecular weights of approximately 1 and approximately 0.5 kDa were present in only three peaks. Strong association of polysaccharides and proteins was observed. Infrared results revealed the presence of one type of polysaccharide and two types of proteins (A and B). Proteins differed mainly in the length of their associated alkyl chains and in the ratio of ester/acidic functionalities.
Laboratoire Environnement et Minéralurgie, Ecole Nationale Supérieure de Géologie, Institut National Polytechnique de Loraine, UMR CNRS 7569, 15 Avenue du Charmois, BP 40, 54501 Vandoeuvre lès Nancy Cedex, France. tatiana.gorner@ensic.inpl-nancy.fr
Tatiana
T
Görner
Philippe
P
de Donato
Marie Hélène
MH
Ameil
Emmanuelle
E
Montarges-Pelletier
Bruno S
BS
Lartiges
D002850:Chromatography, Gel; D005427:Flocculation; D011108:Polymers / Q000302:isolation & purification*; D011134:Polysaccharides / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification; D012722:Sewage; D017550:Spectroscopy, Fourier Transform Infrared; D018508:Water Purification
D011108:Polymers; D011134:Polysaccharides; D011506:Proteins; D012722:Sewage