Presence of glyoxylate cycle enzymes in the mitochondria of Euglena gracilis.
Isocitrate lyase and malate synthase are specific enzymes of the glyoxylate cycle, used here as glyoxysomal markers. Both enzymes were found in the mitochondrial fraction after organelle fractionation by isopycnic centrifugation. Electron microscopy of this fraction indicated that mitochondria were the only recognizable organelles. Using an immunogold labeling method with anti-(malate synthase) antiserum, the only organelles stained in cells were the mitochondria. These results show that the glyoxylate cycle is present in mitochondria in Euglena.
Ono
Kouki
K
Department of Food and Nutrition, Gifu City Women's College, Gifu, Gifu 501-0192, Japan. kono@gifu-cwc.ac.jp
Kondo
Maki
M
Osafune
Tetsuaki
T
Miyatake
Kazutaka
K
Inui
Hiroshi
H
Kitaoka
Shozaburo
S
Nishimura
Mikio
M
Nakano
Yoshihisa
Y
D000818:Animals; D002500:Centrifugation, Isopycnic; D005056:Euglena gracilis / Q000201:enzymology* / Q000648:ultrastructure; D006038:Glyoxylates / Q000378:metabolism*; D007150:Immunohistochemistry; D007522:Isocitrate Lyase / Q000378:metabolism*; D008292:Malate Synthase / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology* / Q000648:ultrastructure
D006038:Glyoxylates; D008292:Malate Synthase; D007522:Isocitrate Lyase