Fluorimetric estimation of DNA content using sensitized lanthanide fluorescence.
Lanthanide fluorescence enhancement on complexation with calf thymus DNA was studied in aqueous solution. The DNA sensitized and enhanced fluorescence of terbium and europium by nearly two orders of magnitude. By applying this ligand sensitized lanthanide fluorescence enhancement, DNA could be estimated at 10 ppb level. Further, effect of addition of TOPO in Triton X-100 micellar medium to Tb-DNA complex in solution was also studied. On addition of TOPO, no synergistic terbium fluorescence enhancement was observed.
Reactor Operations Division, Indira Gandhi Center for Atomic Research, Kalpakkam 603102, Tamilnadu, India. bsp@igcar.ernet.in
B S
BS
Panigrahi
N
N
Gajendran
N
N
Suryamurthy
D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry; D003902:Detergents / Q000494:pharmacology; D005063:Europium / Q000737:chemistry; D005682:Gadolinium / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D008823:Micelles; D017830:Octoxynol / Q000494:pharmacology; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013725:Terbium / Q000737:chemistry; D013950:Thymus Gland / Q000378:metabolism
D003902:Detergents; D028581:Lanthanoid Series Elements; D008823:Micelles; D013725:Terbium; D005063:Europium; D017830:Octoxynol; D004247:DNA; D005682:Gadolinium