[Effect of HIV-1 infection on apoptosis of CD4+ T lymphocytes mediated by Fas].
To study the pathogenic role of Fas/CD95 in HIV-1 infection subjects, and to investigate the effects of HIV on plasma levels of sFas and the expression of CD95 on different CD4(+) T lymphocyte subpopulations.



Four-color flow cytometry was used to determine the expression of CD95, CD45RO, CD45RA on CD4(+ )T lymphocyte in peripheral blood from HIV-1 infection subjects and serum Fas levels were quantified by enzyme-linked immunosorbent assay (ELISA).



Compared with healthy controls, serum Fas levels were significantly increased (P<0.05) in HIV group and positively correlated with the disease progress. The expression of CD95 on naive T-lymphocyte subsets was increased whereas that on memory T-lymphocyte subsets was decreased.



Fas plays an important role in the deletion of CD4(+) T-lymphocyte during HIV-1 infection. Further understanding of the relationship between Fas/CD95 and CD45RO/CD45RA may help to predict the progression of the disease and the clinical outcome.
Wu
Nan-ping
NP
The Institute of Infectious Disease, The First Affiliated Hospital, College of Medical Sciences, Zhejiang Univesity, Hangzhou 310006, China.
Li
Dan
D
Bader
Armin
A
Hoxtermann
Stefan
S
Brockmeyer
Norbert
N
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology*; D000328:Adult; D017209:Apoptosis; D015496:CD4-Positive T-Lymphocytes / Q000473:pathology*; D005260:Female; D015497:HIV-1; D006801:Humans; D017493:Leukocyte Common Antigens / Q000097:blood; D008297:Male; D008875:Middle Aged; D019014:fas Receptor / Q000097:blood / Q000502:physiology*
D019014:fas Receptor; D017493:Leukocyte Common Antigens