Reduced blood-to-tissue albumin movement after plasmapheresis.
We tested the hypothesis that a decrease in the blood-to-tissue movement of albumin contributes to the recovery of plasma albumin and plasma volume after acute plasma protein depletion (plasmapheresis). Awake and unrestrained male Sprague-Dawley rats (220-320 g) fitted with jugular catheters were plasmapheresed, and plasma volume, plasma albumin, and total plasma protein content were measured at 1, 5, 24, and 48 h postplasmapheresis. Plasma volume recovered to baseline within 1 h (4.6 +/- 0.42 vs. 4.7 +/- 0.46 mL/100 g body weight (bw), remained at baseline from 5 h to 24 h but increased to 5.5 + 0.57 mL/100 g bw at 48 h (P < 0.05). Plasma albumin and total protein content recovered rapidly but remained below baseline levels at 1 h (10.05 +/- 0.98 vs. 12.33 +/- 1.29 and 19.75 +/- 1.75 vs. 24.73 +/- 2.56 mg/100 g bw, respectively). Plasma protein content retumed to baseline by 5 h of recovery. Tissue uptake of I125-labeled albumin decreased in the heart, skin, skeletal muscle, and small Intestines of plasmapheresed rats (P < 0.05). These data support the hypothesis that a reduction in albumin efflux from the vascular space contrlbutes to the recovery of plasma albumin and total protein content during plasma volume recovery and eventual expansion after plasmapheresis.
The John B. Pierce Laboratory, Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06519, USA.
Andrea M
AM
Stahl
Christopher M
CM
Gillen
Akira
A
Takamata
Ethan R
ER
Nadel
Gary W
GW
Mack
D000450:Aldosterone / Q000097:blood; D000818:Animals; D001794:Blood Pressure; D001798:Blood Proteins / Q000378:metabolism; D003345:Corticosterone / Q000097:blood; D004573:Electrolytes / Q000097:blood; D006339:Heart Rate; D006728:Hormones / Q000097:blood*; D007328:Insulin / Q000097:blood; D008297:Male; D023421:Models, Animal; D010953:Plasma Volume; D010956:Plasmapheresis / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012709:Serum Albumin / Q000378:metabolism*; D013997:Time Factors
D001798:Blood Proteins; D004573:Electrolytes; D006728:Hormones; D007328:Insulin; D012709:Serum Albumin; D000450:Aldosterone; D003345:Corticosterone