Short-term exercise and platelet activity, sensitivity to agonist, and platelet-leukocyte conjugate formation.
Strenuous exercise may be partially responsible for cardio-vascular events. The aim was to investigate the platelet activity, reactivity and different platelet-leukocyte-conjugate formation following maximal short-term exercises. Fifteen healthy non-smokers underwent three isokinetic maximal tests on a SRM cycle ergometry system with durations of 15, 45 and 90 s. Blood samples were taken after a 30-min rest, immediately before and after exercise, and 15 min and 1 h after completion of exercise. Platelets were detected flow-cytometrically by CD41, and activated platelets by CD62P. In addition, stimulation of the platelets in vitro with 7.5 microM TRAP-6 was initiated. For testing platelet-leukocyte-conjugates, antibodies against CD45, CD14 and CD41 were used. After the exercise tests the percent of non-stimulated CD62P-positive platelets (%PC) was unchanged. In contrast, an increase in %PC (CD62P) TRAP-6 stimulated (15-s test: 37.2+/-10.3 to 46.2+/-12.3%, P < 0.05; 90-s test: 40.6+/-9.5 to 51.7+/-10.2%, P < 0.01) and in platelet-granulocyte, platelet-lymphocyte, and platelet-monocyte conjugate formation 15 min after exercise (45- and 90-s test; P < 0.05) were observed in comparison with the changes on the control day. The changes nearly reversed 1 h after exercise. Maximal short-term exercise only leads to a moderate increase of platelet reactivity and to an increase in the different platelet-leukocyte conjugates. The implications of the changes in platelet-leukocyte conjugate formation should be investigated in future studies.
Hilberg
Thomas
T
Department of Sports Medicine, Friedrich-Schiller-University Jena, Germany. t.hilberg@ghz.de
Glä
Doreen
D
Schmidt
Volker
V
Lösche
Wolfgang
W
Franke
Gabriele
G
Schneider
Kathrin
K
Gabriel
Holger H W
HH
D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002448:Cell Adhesion; D015444:Exercise / Q000502:physiology*; D006098:Granulocytes / Q000166:cytology; D006801:Humans; D007962:Leukocytes / Q000166:cytology*; D008214:Lymphocytes / Q000166:cytology; D008297:Male; D009000:Monocytes / Q000166:cytology; D010446:Peptide Fragments / Q000494:pharmacology; D015539:Platelet Activation / Q000187:drug effects; D013997:Time Factors
D015703:Antigens, CD; D010446:Peptide Fragments; C082835:thrombin receptor peptide (42-47)