[Induction of endo-1,4-beta-xylanase and beta-galactosidase in the original and recombinant strains of the fungus Penicillium canescens].
The induction of the synthesis of secreted enzymes endo-1,4-beta-xylanase (EC 3.2.1.8) and beta-galactosidase (EC 3.2.1.23) in the original and recombinant Penicillium canescens strains has been studied. In all producer strains, the synthesis of these enzymes was induced by arabinose and its metabolite arabitol. The two enzymes differed in the concentration of arabinose required for the induction: the synthesis of beta-galactosidase was most pronounced at 1 mM, whereas maximum synthesis of endo-1,4-beta-xylanase was observed at 5 to 10 mM. An increase in the number of endo-1,4-beta-xylanase copies in the high-copy-number strain of the fungus suppressed the synthesis of beta-galactosidase; the synthesis of endo-1,4-beta-xylanase in the high-copy-number recombinant producing beta-galactosidase was affected to a lesser extent. The amount of the enzymes synthesized did not depend on the saccharide used as a sole source of carbon for growing the mycelium prior to its transfer to the inducer-containing medium.
Vavilova
E A
EA
All-Russian Research Institute of Genetics and Selection of Industrial Microorganisms, Pervyi Dorozhnyi proezd 1, Moscow, 113545 Russia.
Vinetskiĭ
Iu P
IuP
D001089:Arabinose / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D043364:Endo-1,4-beta Xylanases; D010407:Penicillium / Q000201:enzymology* / Q000235:genetics; D011995:Recombination, Genetic; D013402:Sugar Alcohols / Q000494:pharmacology; D014995:Xylosidases / Q000096:biosynthesis*; D001616:beta-Galactosidase / Q000096:biosynthesis*
D013402:Sugar Alcohols; D001089:Arabinose; D014995:Xylosidases; D001616:beta-Galactosidase; D043364:Endo-1,4-beta Xylanases; C014999:arabitol