Developmental effects of serum from flaxseed-fed rats on cultured rat embryos.
Gestation day 9.5 rat embryos were cultured for 45 h in serum obtained from pregnant rats that had been fed throughout gestation with either a control diet (based on the AIN-93 formulation), a diet supplemented with flaxseed (20% or 40%, w/w), or a diet supplemented with de-fatted flaxseed ("flaxseed meal", 13 or 26%, w/w). The embryos were fixed in neutral formalin at the end of culture. Overall growth and development was assessed, and the presence of abnormalities was noted. A significant inhibition of growth (as determined by crown-rump length) relative to control was observed in embryos cultured in serum from rats fed the 20% flaxseed diet. The incidence of spontaneous heart inversions was increased significantly in the embryos cultured in serum from the 20% flaxseed and 26% flaxseed meal fed rats. The incidence of flexion defects was increased significantly in embryos cultured in serum from 20% flaxseed-fed rats. The lack of an apparent dose response in any of the statistically significant effects suggests that the observed anomalies were chance occurrences unrelated to the treatment group from which serum was obtained. It is therefore concluded that diets high in flaxseed or flaxseed meal do not result in serum factors that are directly embryotoxic to organogenesis-staged rat embryos. This finding is consistent with the findings of a parallel in vivo rat teratology study where no significant embryotoxicity attributable to flaxseed exposure was observed.
Offices of Applied Research and Safety Assessment, FDA, MOD-1 Laboratories, 8301 Muirkirk Road, Laurel, MD 20708, USA. tflynn@cfsan.fda.gov
T J
TJ
Flynn
T F X
TF
Collins
R L
RL
Sprando
T N
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Black
D I
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Ruggles
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Wiesenfeld
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Babu
D000014:Abnormalities, Drug-Induced; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004622:Embryo, Mammalian / Q000187:drug effects*; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D005260:Female; D019597:Flax / Q000633:toxicity*; D009024:Morphogenesis / Q000187:drug effects; D009924:Organ Culture Techniques; D011247:Pregnancy; D051381:Rats; D012639:Seeds / Q000633:toxicity*; D015430:Weight Gain / Q000187:drug effects