Tissue-culture surfaces with mixtures of aminated and fluorinated functional groups. Part 2. Growth and function of transgenic rat insulinoma cells (betaG I/17).
Interactions of transplantable cells with synthetic polymers can influence the function of biohybrid artificial organs. This study explored growth and secretion of human insulin by betaG I/17 cells cultured on surfaces bearing diamine groups (N2), trifluoropropyl groups (F3) and mixtures of the two. Cells cultured on high-F3 and high-N2 surfaces spread well, grew rapidly and produced >1.8 mol lactate per mol glucose consumed, closely resembling cells grown on the permissive control, glass. On one mixed surface, with a molar ratio of 33 N2 groups:67 F3 groups, cells had a lower lactate/glucose ratio, adopted a rounded form, grew slowly and were quick to form emergent aggregates, similar to cultures on the inhibitory control, untreated polystyrene. Cultures on surfaces with higher F3 content secreted the most insulin and, in the case of the highest-F3 surface, showed improved responsiveness to secretagogues. Hormone secretion was roughly 50% greater when cells were grown on F3 surfaces conditioned by earlier cultures of betaG I/17. Incubation of conditioned surfaces with high concentrations of a polyclonal anti-laminin serum prior to re-plating partially abolished this improvement in secretory function. Polymers bearing trifluoropropyl groups appear to be attractive candidates for use in the artificial endocrine pancreas. Surface coatings that include laminin might promote function of transgenic insulinoma cells in vitro and in vivo.
Bain
James R
JR
Sarah W. Stedman Center for Nutritional Studies, Duke University Medical Center, Durham, NC 27710, USA. james.bain@duke.edu
Hoffman
Allan S
AS
D000818:Animals; D030801:Animals, Genetically Modified; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology*; D046508:Culture Techniques / Q000295:instrumentation; D003959:Diamines; D005461:Fluorine; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007340:Insulinoma / Q000378:metabolism / Q000473:pathology*; D011384:Proinsulin / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D051381:Rats; D012821:Silanes; D023822:Tissue Engineering / Q000295:instrumentation*
D020099:Coated Materials, Biocompatible; D003959:Diamines; D012821:Silanes; D005461:Fluorine; D011384:Proinsulin; D005947:Glucose