Premature prostaglandin F2alpha secretion causes luteal regression in GnRH-induced short estrous cycles in cyclic dairy heifers.
This study aimed to confirm that the luteolysis in normal-cycling dairy heifers seen during short estrous cycles induced with cloprostenol (Clp) and GnRH administered 24h apart is caused by a premature release of prostaglandin F(2alpha) (PGF(2alpha)). A further aim was to study the PGF(2alpha) release pattern more closely to determine whether it resembles the spontaneous release occurring during normal regression of the corpus luteum (CL) or whether PGF(2alpha) is continuously secreted after the induced ovulations, leading to short estrous cycles. Twenty-four Ayrshire heifers were allotted to four equally sized groups. After estrus synchronization with 0.5mg of Clp, a new luteolysis was induced with 0.5mg of Clp on Day 6 (groups T-d6 and C-d6) or Day 7 (groups T-d7 and C-d7) after ovulation. Gonadorelin (0.1mg i.m.) was given to groups T-d6 and T-d7 to induce premature ovulation 24h later. Groups C-d6 and C-d7 served as controls. Ovaries were examined daily by transrectal ultrasonography, while blood samples (for progesterone and 15-ketodihydro-PGF(2alpha) analyses) were obtained via a jugular catheter every 3h, starting from the second Clp treatment and continuing for 9 days postovulation. Unresponsiveness to Clp or anovulation resulted in 4 C-d6 heifers being excluded. Four heifers in group T-d6 and three in group T-d7 had a short estrous cycle of 8-12 days, while all others had a cycle of normal length. Significant elevations in 15-ketodihydro-PGF(2alpha) concentrations with recurrent high peaks coincided with a decrease in progesterone concentration and were detected in all heifers that showed a short estrous cycle, but not in any heifers with normal estrous cycles in groups T and C. In conclusion, a premature release of PGF(2alpha), which closely resembles its release during spontaneous luteolysis, causes luteal regression in these short cycles.
Department of Clinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Helsinki, Saari Unit, Pohjoinen pikatie 800, 04920 Saarentaus, Mäntsälä, Finland. juhani.taponen@helsinki.fi
Juhani
J
Taponen
Pia
P
Hjerppe
Eija
E
Kopra
Heriberto
H
Rodríguez-Martínez
Terttu
T
Katila
Hans
H
Kindahl
D000818:Animals; D002417:Cattle / Q000502:physiology*; D003008:Cloprostenol / Q000008:administration & dosage; D003338:Corpus Luteum / Q000502:physiology; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism*; D030762:Estrous Cycle / Q000187:drug effects*; D004973:Estrus Synchronization; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000008:administration & dosage*; D007700:Kinetics; D003341:Luteolysis / Q000502:physiology*; D006080:Ovarian Follicle / Q000502:physiology; D010053:Ovary / Q000000981:diagnostic imaging; D010060:Ovulation / Q000187:drug effects; D011374:Progesterone / Q000097:blood; D014463:Ultrasonography
D007987:Gonadotropin-Releasing Hormone; C026528:15-ketoprostaglandin F2alpha; D003008:Cloprostenol; D011374:Progesterone; D015237:Dinoprost