Effect of artificial saliva contamination on pH value change and dentin bond strength.
OBJECTIVE
This study was to examine the effect of artificial saliva contamination on pH change of the dentin surface and the micro-shear bond strength (MSBS) of the two bonding systems to contaminated dentin.


METHODS
Fifty-six human dentin disks were tested with two resin bonding systems: a self-etching primer system, Clearfil SE Bond (Kuraray Medical Inc., Tokyo, Japan), and a one-bottle adhesive system, Single Bond (3M-ESPE, St. Paul, MN). Dentin surfaces were conditioned with the self-etching primer (primer) or phosphoric acid (etchant) and divided into four groups: conditioning without contamination (conditioning), contamination with artificial saliva (contamination), re-conditioning the contaminated dentin (re-conditioning), water-rinsing the contaminated dentin and re-conditioning (rinsing and re-conditioning). The pH change on the dentin surface was measured using a pH-imaging microscope (SCHEM-100, Horiba Ltd, Kyoto, Japan) to estimate the acid-base characteristics of the conditioned and contaminated dentin surface. The MSBS to the dentin was examined after storage in distilled water at 37 degrees C for 1 week.


RESULTS
The pH of intact dentin surfaces was 6.9. Conditioning with the primer and etchant decreased the pH to 5.4 and 5.9, respectively. Saliva contamination increased the pH slightly, and re-conditioning decreased the pH again. The MSBS of the two bonding systems decreased after contamination. Re-priming restored the MSBS to control values, while re-etching did not. Rinsing and re-priming produced a reduction in MSBS, and rinsing and re-etching did not increase the MSBS.


CONCLUSIONS
The conditioning and saliva contamination changed the pH value of dentin surface. The MSBS decreased after contamination; however, re-priming with SE Bond primer was an adequate treatment to restore the bond strength.
Cariology and Operative Dentistry, Department of Restorative Sciences, Graduate School of Tokyo Medical and Dental University, Tokyo, Japan. noricoh@aol.com
Noriko
N
Hiraishi
Yuichi
Y
Kitasako
Toru
T
Nikaido
Satoshi
S
Nomura
Michael F
MF
Burrow
Junji
J
Tagami
D000134:Acid Etching, Dental; D017438:Bisphenol A-Glycidyl Methacrylate; D002627:Chemistry, Physical / Q000295:instrumentation; D001840:Dental Bonding; D003804:Dentin / Q000737:chemistry*; D017220:Dentin-Bonding Agents; D006801:Humans; D006863:Hydrogen-Ion Concentration; D019279:Resin Cements; D012464:Saliva, Artificial; D012666:Semiconductors; D033081:Shear Strength; D012815:Signal Processing, Computer-Assisted; D013499:Surface Properties
C438313:Clearfil SE Bond; D017220:Dentin-Bonding Agents; D019279:Resin Cements; D012464:Saliva, Artificial; C115612:single bond; D017438:Bisphenol A-Glycidyl Methacrylate