Isolating ligands specific for human vasculature using in vivo phage selection.
The endothelium lining blood vessels expresses molecules that are restricted in their expression to a particular tissue or organ. These molecules are attractive targets for therapy and diagnosis because they allow agents to be delivered specifically to the blood vessels supplying the desired tissue. However, it is difficult to identify these tissue-specific molecules because endothelium loses much of its tissue-specific nature when it is removed from the organ. This can be overcome by using in vivo phage selection - injecting libraries of phage bearing antibodies or peptides into an animal and isolating phage that bind to the relevant tissue. A variation on this approach, in which in vivo phage selection is performed in animals bearing human tissue xenografts, allows the isolation of peptides (and presumably other molecules) specific for human vasculature.
Department of Immunology, Division of Medicine, Imperial College London, Hammersmith Hospital, UK. a.george@imperial.ac.uk
Andrew J T
AJ
George
Lewis
L
Lee
Costantino
C
Pitzalis
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D046508:Culture Techniques / Q000379:methods*; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000637:transplantation*; D006801:Humans; D008024:Ligands; D051379:Mice; D016513:Mice, SCID; D019151:Peptide Library; D040081:Protein Array Analysis / Q000379:methods*; D011506:Proteins / Q000378:metabolism*; D016378:Tissue Transplantation / Q000379:methods*; D014183:Transplantation, Heterologous
D015415:Biomarkers; D008024:Ligands; D019151:Peptide Library; D011506:Proteins