Effect of media on spore yield and thermal resistance of Bacillus stearothermophilus.
The interference of eight components in the yield of sporulation and thermal resistance to moist heat (121 degrees C) of Bacillus stearothermophilus spores suspended in 0.02 M calcium acetate solution and inoculated on paper strips previously treated with calcium acetate/calcium hydroxide was studied. The spore yield of 1.0 x 10(8)mL was developed at 62 degrees C in 17 media containing different concentrations of D-glucose, sodium chloride, L-glutamic acid, yeast extract, peptone, manganese sulfate, potassium phosphate, and ammonium phosphate. The combined effects of yeast extract, peptone, and glucose contributed positively to the spore yield and to the stability of the thermal resistance of both spores in suspension and on strips.
Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Science, University of São Paulo, Rua Antonio de Macedo Soares, 452 04607-000, São Paulo/SP, Brazil. tcvpenna@usp.br
Thereza Christina Vessoni
TC
Penna
Irene A
IA
Machoshvili
Marina
M
Ishii
D003470:Culture Media; D001411:Geobacillus stearothermophilus / Q000254:growth & development / Q000502:physiology*; D006358:Hot Temperature; D007700:Kinetics; D013171:Spores, Bacterial / Q000254:growth & development*; D013816:Thermodynamics
D003470:Culture Media