Construction of the mobilizable plasmid pMV158GFP, a derivative of pMV158 that carries the gene encoding the green fluorescent protein.
Plasmid pMV158 has been employed to construct cloning non-mobilizable vectors for various Gram-positive organisms. Here we report the construction of a mobilizable pMV158-based plasmid that harbors the gene encoding the green fluorescent protein under the control of a promoter inducible by maltose. The plasmid was mobilized between strains of Streptococcus pneumoniae as well as from S. pneumoniae to Lactococcus lactis or Enterococcus faecalis at the same frequency as its parental. Transconjugant that received the GFP-tagged plasmid could be detected by their fluorescence, which was especially high in E. faecalis cells.
Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Velázquez, 144, E-28006 Madrid, Spain.
Concepción
C
Nieto
Manuel
M
Espinosa
D003227:Conjugation, Genetic; D013293:Enterococcus faecalis / Q000235:genetics; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D013294:Lactococcus lactis / Q000235:genetics; D008164:Luminescent Proteins / Q000235:genetics* / Q000378:metabolism; D008320:Maltose / Q000235:genetics; D008856:Microscopy, Fluorescence; D008858:Microscopy, Phase-Contrast; D010957:Plasmids / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D013296:Streptococcus pneumoniae / Q000235:genetics; D014466:Ultraviolet Rays
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins; D008320:Maltose