Genetic organization of genes encoding phenol hydroxylase, benzoate 1,2-dioxygenase alpha subunit and its regulatory proteins in Acinetobacter calcoaceticus PHEA-2.
Acinetobacter calcoaceticus PHEA-2 is a phenol-degrading bacterium isolated from the wastewater from an oil refinery. A 10-kb XhoI fragment consisting of nine complete Open Reading Frames (ORFs) and one partial ORF was screened from a lambda library of PHEA-2 by Southern hybridization. The sequence analyses revealed that ORF2-ORF7, designated mphKLMNOP, are homologous to dmpKLMNOP of Pseudomonas sp. CF600 and mopKLMNOP of Acinetobacter calcoaceticus NCIB8250, sharing 38%-72% and 58.5%-93.5% respectively. The products encoded by dmp and mop genes convert phenol to catechol. The mph-operon and downstream ORFs, ORF9 and ORF10, sharing high identities to benM and benA, which encode ben-operon regulatory protein and benzoate 1,2-dioxygenase alpha subunit respectively, are separated by ORF8, whose function is unknown. The organization of the mph and ben operons is different from that described previously.
Xu
Yuquan
Y
Department of Biotechnology, Institute for Application of Atomic Energy, Chinese Academy of Agricultural Sciences, P. O. Box 5109, Beijing, 100094, China.
Chen
Ming
M
Zhang
Wei
W
Lin
Min
M
D016954:Acinetobacter calcoaceticus / Q000201:enzymology* / Q000235:genetics*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D040461:Gene Components; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D005798:Genes, Bacterial; D006824:Hybridization, Genetic; D006899:Mixed Function Oxygenases / Q000235:genetics*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010105:Oxygenases / Q000235:genetics*; D016415:Sequence Alignment
D001426:Bacterial Proteins; D006899:Mixed Function Oxygenases; D010105:Oxygenases; C019766:benzoate 1,2-dioxygenase; C022336:phenol 2-monooxygenase