"Two-in-one" gel for spot matching after two-dimensional electrophoresis.
Two-dimensional electrophoresis (2-DE) is one of the most commonly used techniques in proteomic investigations. However, due to the complex interplay of incidence including significant biological sample variations, lengthy steps involved in performing 2-DE as well as exposure time with silver staining, it is sometimes difficult to differentiate authentic differences caused by drug treatment with those artifacts caused by sample variations, running conditions of 2-DE as well as treatment time in silver staining etc. If we can compare pooled samples of control and treatment groups run in a single gel and stained together, we would be more comfortable with our findings. We propose here a low cost and highly effective method for locating differentially expressed proteins before and after drug treatment. This "two-in-one gel" technique might partially solve the problems mentioned above.
Wang
Yuen-Yuen
YY
The Proteomic Task Force, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, China.
Cheung
Pik-Yuen
PY
Wong
Man-Sau
MS
Lo
Samuel Chun-Lap
SC
D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology; D011506:Proteins / Q000302:isolation & purification; D040901:Proteomics / Q000379:methods*; D051381:Rats; D012834:Silver; D013154:Spleen / Q000737:chemistry / Q000166:cytology / Q000187:drug effects; D013194:Staining and Labeling
D008070:Lipopolysaccharides; D011506:Proteins; D003208:Concanavalin A; D012834:Silver