Mucosally-derived HPV-40 can infect both human genital foreskin and cutaneous hand skin tissues grafted into athymic mice.
HPV-40 is a rare HPV type that has been detected only in genital mucosal tissues. This HPV type is very closely related to HPV-7, which has a predominantly cutaneous tissue tropism. We have shown, previously, that an isolate of HPV-40 (described here as HPV-40(Hershey) or HPV-40(H)) productively infected genital tissues. In this study, HPV-40(H) was tested for productive infection of cutaneous tissue. Fetal hand skin fragments were incubated with infectious HPV-40(H) and implanted subrenally into athymic mice. After 120 days, xenografts showed morphological changes consistent with HPV-40(H) infection and were HPV-40 DNA in situ positive and capsid antigen positive. The results demonstrated that hand skin can support HPV-40(H) infection thereby indicating that this viral type has the capacity to infect both genital mucosal and cutaneous tissues.
Jenkins
Audrey L
AL
Department of Comparative Medicine, College of Medicine, The Milton S. Hershey Medical Center of the Pennsylvania State University, 500 University Drive, Hershey, PA 17033, USA.
Lang
C Max
CM
Budgeon
Lynn R
LR
Cladel
Nancy M
NM
Reed
Cynthia A
CA
Welch
Danny R
DR
Christensen
Neil D
ND
Jenkins
Audrey J
AJ
D000595:Amino Acid Sequence; D000818:Animals; D016332:Fetal Tissue Transplantation / Q000473:pathology; D006225:Hand; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data; D009092:Mucous Membrane / Q000821:virology*; D027383:Papillomaviridae / Q000302:isolation & purification / Q000472:pathogenicity*; D010413:Penis / Q000821:virology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012867:Skin / Q000473:pathology / Q000821:virology*; D016038:Skin Transplantation / Q000473:pathology*; D014183:Transplantation, Heterologous / Q000473:pathology; D014764:Viral Proteins / Q000737:chemistry*
D014764:Viral Proteins