Comparison of real-time PCR detection methods for B1 and P30 genes of Toxoplasma gondii.
Real-time PCR assays were established with the Toxoplasma gondii P30 and the 35-copy B1 gene as target genes. Both PCR methods detected Toxoplasma DNA from 10 to 100000 genome equivalents per assay and had comparable intra-assay deviations suggesting that detecting a single copy locus is suitable for clinical diagnostic.
Institute for Medical Microbiology, University of Leipzig, Liebigstrasse 24, 04103 Leipzig, Germany. Buchsann@gmx.de
Susanne
S
Buchbinder
Rosemarie
R
Blatz
Arne Christian
AC
Rodloff
D000293:Adolescent; D000328:Adult; D000818:Animals; D000953:Antigens, Protozoan; D001483:Base Sequence; D005260:Female; D017125:Genes, Protozoan / Q000235:genetics*; D018773:Genes, erbB-1 / Q000235:genetics*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D015800:Protozoan Proteins / Q000235:genetics*; D012680:Sensitivity and Specificity; D014122:Toxoplasma / Q000235:genetics* / Q000302:isolation & purification; D014123:Toxoplasmosis / Q000175:diagnosis
D000953:Antigens, Protozoan; D015800:Protozoan Proteins; C047168:SAG1 antigen, Toxoplasma