Pilot study of COBAS PCR and ligase chain reaction for detection of rectal infections due to Chlamydia trachomatis.
We tested rectal specimens from men who have sex with men for Chlamydia trachomatis by using COBAS PCR (Roche Diagnostics) and ligase chain reaction LCR (Abbott laboratories) and compared three PCR specimen-processing procedures. Chlamydiae were detected by one or more procedures in 22 of 186 specimens. All three PCR tests were positive for 17 specimens, all of which also tested positive by LCR.
Golden
Matthew R
MR
Division of Infectious Diseases and the Center for AIDS & STD, University of Washington, and Public Health-Seattle and King County STD Program, Seattle, USA. golden@u.washington.edu
Astete
Sabina G
SG
Galvan
Rosa
R
Lucchetti
Aldo
A
Sanchez
Jorge
J
Celum
Connie L
CL
Whittington
William L H
WL
Stamm
Walter E
WE
Holmes
King K
KK
Totten
Patricia A
PA
D002690:Chlamydia Infections / Q000175:diagnosis* / Q000382:microbiology / Q000635:transmission; D002692:Chlamydia trachomatis / Q000235:genetics; D018451:Homosexuality, Male; D006801:Humans; D022521:Ligase Chain Reaction / Q000379:methods*; D008297:Male; D010865:Pilot Projects; D016133:Polymerase Chain Reaction / Q000379:methods*; D012002:Rectal Diseases / Q000175:diagnosis* / Q000382:microbiology