Proliferating cell nuclear antigen in testes of infertile men with varicocele--preliminary results of interrelationship with sperm count before and after varicocelectomy.
OBJECTIVE
This study was conducted to assess germ cell kinetics and correlate them with sperm counts before and after varicocelectomy.


METHODS
Forty-three testicular biopsy specimens were obtained from 47 patients with varicocele during varicocelectomy. Similar specimens were obtained from 8 fertile volunteers. All specimens were immunostained using anti-proliferating cell nuclear antigen (anti-PCNA) antibody. PCNA expression was evaluated by assessing its staining intensity (SI) and labeling index (LI).


RESULTS
The varicocele specimens revealed significantly lower SI and LI than the controls. There was a significant correlation between initial sperm concentration and LI but not SI. Coexistence of LI > or = 31 and preoperative sperm count > 1 million/ml correlated with a significant rise in the postoperative sperm count.


CONCLUSIONS
PCNA is a useful molecular marker for assessing germ cell kinetics in varicocele patients. The decline in DNA synthesis, as suggested by the lower PCNA SI and LI in varicocele specimens, could be a reason for the disordered spermatogenesis in these patients. PCNA LI may also be considered as a beneficial clinical marker and may help to predict the surgical outcome after varicocele repair.
Department of Urology, Tokushima School of Medicine, Tokushima City, Japan. nadersalama@hotmail.com
Nader
N
Salama
Masahito
M
Tsuji
Masato
M
Tamura
Susumu
S
Kagawa
D000328:Adult; D006801:Humans; D007248:Infertility, Male / Q000209:etiology / Q000473:pathology* / Q000601:surgery*; D008297:Male; D017063:Outcome Assessment, Health Care; D011237:Predictive Value of Tests; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis*; D013076:Sperm Count; D013091:Spermatogenesis / Q000502:physiology; D013094:Spermatozoa / Q000502:physiology; D013737:Testis / Q000473:pathology* / Q000503:physiopathology / Q000601:surgery*; D014646:Varicocele / Q000150:complications / Q000473:pathology* / Q000601:surgery*
D018809:Proliferating Cell Nuclear Antigen