Human B-blast specific target determinants in CML: a panel study.
The B blast specific target determinant phenotype defined by cytotoxic lymphocytes and the HLA-DR phenotype was tested for correlation in two panel studies. First, CTL reagents with B blast specificity were selected from CTLs educated between HLA-A, B, (C) identical unrelated donors giving no cytotoxicity with specific T blasts, resulting in free selection of panel B blasts used as targets. Second, CTLs educated between HLA non-identical unrelated donors giving cytotoxicity against specific T as well a B blast targets could be seen with negative reactions against T blasts but positive reactions against B blasts of selected panel members giving, in this set up, a free selection of donors cocultured for education of CTLs. These panel studies showed that HLA-DR associated B blast specific target determinants can be identified by CTLs, indicating that the HLA system codes for these determinants either through gene products of a locus closely linked to or identical with the HLA-DR locus.
The Tissue Typing Laboratory, Blood Bank and Blood Grouping Laboratory, Aarhus Kommunehospital, University of Aarhus, Denmark.
H E
HE
Johnsen
D001402:B-Lymphocytes / Q000737:chemistry* / Q000276:immunology*; D015234:HLA-A Antigens / Q000032:analysis / Q000276:immunology; D015235:HLA-B Antigens / Q000032:analysis / Q000276:immunology; D015236:HLA-C Antigens / Q000032:analysis / Q000276:immunology; D006684:HLA-DR Antigens / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology*; D007156:Immunologic Memory; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D015234:HLA-A Antigens; D015235:HLA-B Antigens; D015236:HLA-C Antigens; D006684:HLA-DR Antigens