Capillary electrophoresis with end-column amperometric detection of urinary 8-hydroxy-2'-deoxyguanosine.
Urinary 8-hydroxy-2'-deoxyguanosine (8OHdG) is an excellent marker of oxidative DNA damage. Until now, urinary 8OHdG has been measured by high-performance liquid chromatography with electrochemical detection. A simple and sensitive method for the analysis of urinary 8OHdG by capillary electrophoresis with end-column amperometric detection has been developed in our laboratory. A single-step solid-phase extraction procedure was optimized and used for extracting 8OHdG from human urine. To improve the sensitivity of this method, a new focusing technique based on a dynamic pH junction was used. The limit of detection was 20 nM (signal-to-noise ratio S/N = 3), the linear range was 50 nM-10 microM, and the correlation coefficient was better than 0.999. The relative standard deviation (RSD) was found to be 0.57% for migration time, and 4.79% for peak current. To show the usefulness of the method, the urinary concentration of 8OHdG in nine healthy persons and ten cancer patients was determined. The urinary concentration of 8OHdG in cancer patients was significantly higher than that in healthy persons.
Mei
Su-Rong
SR
National Chromatographic R & A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
Yao
Qing-Hong
QH
Cai
Ling-Suang
LS
Xing
Jun
J
Xu
Guo-Wang
GW
Wu
Cai-Ying
CY
D015415:Biomarkers / Q000652:urine; D016022:Case-Control Studies; D004249:DNA Damage; D003839:Deoxyadenosines / Q000652:urine*; D004581:Electronics; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods / Q000592:standards; D005260:Female; D006801:Humans; D008297:Male; D009369:Neoplasms / Q000652:urine; D015203:Reproducibility of Results
C436623:8-hydroxy-2'-deoxyadenosine; D015415:Biomarkers; D003839:Deoxyadenosines