Effect of fluoridated milk on progression of root surface lesions in vitro under pH cycling conditions.
The aim of this study was to assess the effect of milk with 0, 2.5 or 5 ppm F on progression and remineralization of caries-like root surface lesions using a pH cycling model. The root surface lesions were created utilizing a partially saturated lactic acid buffer at pH 4.6. Longitudinal sections were cut through the lesion and analyzed using polarized light microscopy (PLM) and microradiography (MRG). The sections were then coated with an acid resistant varnish, except the outer natural surface that would be exposed to water, milk or fluoridated milk and cycled in a de- and remineralizing system for 2 weeks. The lesions were characterized again by PLM and MRG after treatment. A significant reduction in lesion progression was found by PLM and MRG after treatment with either non-fluoridated or fluoridated milk when compared to the control group. Using quantitative MRG, mineral change and distribution in the lesions were recorded. A possible protective effect of fluoridated milk on root surface caries was supported by a reduction in the progression of the lesions and an increase in the mineral within the lesion.
Department of Dentistry, University Hospital and Medical Faculty of Charles University, Hradec Kralove, Czech Republic.
R
R
Ivancakova
M M
MM
Hogan
J D
JD
Harless
J S
JS
Wefel
D000704:Analysis of Variance; D000818:Animals; D002417:Cattle; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008852:Microradiography; D008859:Microscopy, Polarization; D008892:Milk / Q000737:chemistry*; D008963:Molar; D017213:Root Caries / Q000517:prevention & control*; D012969:Sodium Fluoride / Q000008:administration & dosage*; D018709:Statistics, Nonparametric; D014089:Tooth Remineralization
D012969:Sodium Fluoride