[Relationship between apoptosis and expression of Fas, bcl-2 and Ki-67 in condyloma acuminatum].
OBJECTIVE
This study inquired into the significance and relationship of apoptosis to the regulation and proliferation of condyloma acuminatum(CA).


METHODS
Apoptotic cells were detected in situ by terminal deoxynucleotidyl transferase-mediated-dUTP nick end labeling (TUNEL). The detection of Fas, bcl-2 and Ki-67 expression was performed using immunohistochemical staining in 28 specimens of CA.


RESULTS
Apoptotic cells were found in 24 cases (85.7%) in keratinocytes. All of the in 28 cases(100%) had Fas and Ki-67 positive expressions, but only 8 cases (28.6%) had bcl-2 mild or moderate expression. The disease severity (serious, moderate and mild) groups did not significantly differ in apoptotic index (AI), Fas, bcl-2 and Ki-67 positive expression (P > 0.05). There was positive correlation between AI and Fas(r = 0.866, P = 0.000). bcl-2 and Ki-67 were negatively correlated with AI (r = -0.416, P = 0.018; r = -0.475, P = 0.006).


CONCLUSIONS
It is suggested that apoptosis in keratinocytes may play an important role in inhibiting the proliferation of virus, that Fas may be an upregulative factor and bcl-2 may be an inhibition factor to apoptosis, and that excessive Ki-67 expression may be associated with the acceleration of cell cycle.
Department of Dermatovenereology, First Affiliated Hospital, WCUMS, Chengdu 610041, China.
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Y
Zhang
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Zhang
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Wang
F
F
Li
D048868:Adaptor Proteins, Signal Transducing; D000328:Adult; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D003218:Condylomata Acuminata / Q000235:genetics / Q000473:pathology*; D005260:Female; D006801:Humans; D019394:Ki-67 Antigen / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics
D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; D002352:Carrier Proteins; C490780:FAF1 protein, human; D019394:Ki-67 Antigen; D019253:Proto-Oncogene Proteins c-bcl-2