[Zinc modulates the Ca(2+)-ATPase activity of rat osteoblast membrane].
The effect and mechanism of zinc on Ca(2+)-ATPase and Na+, K(+)-ATPase activity of rat osteoblast membrane were studied. The activities were measured by colorimetric method. The effect of special inhibitors of protein kinase C or calmodulin on membrane Ca(2+)-ATPase and Na+, K(+)-ATPase activity which induced by zinc were also examined. The results showed that zinc could increase membrane Ca(2+)-ATPase activity, but no influence was observed on Na+, K(+)-ATPase activity. R24571, a special inhibitor of calmodulin, had no effect on membrane Ca(2+)-ATPase activity. Calmodulin also could not promote the basic Ca(2+)-ATPase activity of membrane. But trifluoperazine, a special inhibitor of protein kinase C, obviously reduced its activity. It was concluded that the modulation of zinc on membrane Ca(2+)-ATPase activity of rat osteoblast could be mediated by protein kinase C.
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Medical Experimental Center of First University Hospital, West China University of Medical Sciences, Chengdu 610041, China.
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Wang
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D000818:Animals; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology; D010006:Osteoblasts / Q000201:enzymology*; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D015032:Zinc / Q000494:pharmacology*
D011493:Protein Kinase C; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase; D015032:Zinc