Measurement of plasma procyanidin B-2 and procyanidin B-3 levels after oral administration in rat.
Using a high-performance liquid chromatographic method and mass spectrometry analysis, we successfully measured the absorption of orally administered procyanidin B-2 and procyanidin B-3 isolated from Cinnamonomi cortex (the bark of Cinnamomum cassia Blume) in the rat plasma. This method used a TSK- GEL ODS-80TS column, two solvents (A: 0.01% acetic acid; B: methanol with 0.01% acetic acid) in a linear gradient at a flow-rate of 1.0 ml/min, and fluorescence detection at excitation and emission wavelengths of 220 and 327 nm.
Department of Japanese Oriental Medicine, Toyama Medical and Pharmaceutical University, Toyama, Japan.
N
N
Tanaka
N
N
Sekiya
M
M
Hattori
H
H
Goto
N
N
Shibahara
Y
Y
Shimada
K
K
Terasawa
D000284:Administration, Oral; D000818:Animals; D044946:Biflavonoids; D002392:Catechin / Q000097:blood / Q000737:chemistry / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D032904:Cinnamomum aromaticum; D008297:Male; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000494:pharmacology; D044945:Proanthocyanidins; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D044946:Biflavonoids; D010936:Plant Extracts; D044945:Proanthocyanidins; C479578:procyanidin B; C017674:procyanidin; D002392:Catechin