[Effect of HIV-1 infection on apoptosis of CD4+ T lymphocytes mediated by Fas].
To study the pathogenic role of Fas/CD95 in HIV-1 infection subjects, and to investigate the effects of HIV on plasma levels of sFas and the expression of CD95 on different CD4(+) T lymphocyte subpopulations.



Four-color flow cytometry was used to determine the expression of CD95, CD45RO, CD45RA on CD4(+ )T lymphocyte in peripheral blood from HIV-1 infection subjects and serum Fas levels were quantified by enzyme-linked immunosorbent assay (ELISA).



Compared with healthy controls, serum Fas levels were significantly increased (P<0.05) in HIV group and positively correlated with the disease progress. The expression of CD95 on naive T-lymphocyte subsets was increased whereas that on memory T-lymphocyte subsets was decreased.



Fas plays an important role in the deletion of CD4(+) T-lymphocyte during HIV-1 infection. Further understanding of the relationship between Fas/CD95 and CD45RO/CD45RA may help to predict the progression of the disease and the clinical outcome.
The Institute of Infectious Disease, The First Affiliated Hospital, College of Medical Sciences, Zhejiang Univesity, Hangzhou 310006, China.
Nan-ping
NP
Wu
Dan
D
Li
Armin
A
Bader
Stefan
S
Hoxtermann
Norbert
N
Brockmeyer
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology*; D000328:Adult; D017209:Apoptosis; D015496:CD4-Positive T-Lymphocytes / Q000473:pathology*; D005260:Female; D015497:HIV-1; D006801:Humans; D017493:Leukocyte Common Antigens / Q000097:blood; D008297:Male; D008875:Middle Aged; D019014:fas Receptor / Q000097:blood / Q000502:physiology*
D019014:fas Receptor; D017493:Leukocyte Common Antigens