Phylogenetic analysis of an anaerobic microbial consortium deiodinating 5-amino-2,4,6-triiodoisophthalic acid.
The dehalogenating performance of an anaerobic 5-amino-2,4,6-triiodoisophthalic acid (ATIA) fixed-bed reactor was evaluated. The reactor operating conditions were set for ATIA deiodination. A phylogenetic survey for a stable anaerobic ATIA-deiodinating microbial consortium was carried out using 16S rDNA restriction fragment length polymorphism, and unique clones were sequenced. Four phylotypes were identified. Two sequences were related to those of Desulfitobacterium frappieri species and another was closest to that of Desulfitobacterium hafniense, but may have represented a new Desulfitobacterium species. Desulfitobacteria were previously described as aryl-dechlorinating and debrominating bacteria. The new strains identified in this study were probably responsible for the ATIA deiodination. The fourth clone was related to the Clostridium-Flavobacterium-Bacteroides group.
Lecouturier
D
D
Génie Chimique, Université de Technologie de Compiègne, Centre de Recherche de Royallieu, B.P. 20529, 60205 Compiègne cedex, France.
Godon
J-J
JJ
Lebeault
J-M
JM
D001421:Bacteria, Anaerobic / Q000145:classification* / Q000235:genetics; D019149:Bioreactors; D004275:DNA, Ribosomal / Q000032:analysis; D045858:Desulfitobacterium / Q000145:classification* / Q000235:genetics; D017753:Ecosystem; D007220:Industrial Waste; D007455:Iodine / Q000378:metabolism*; D008969:Molecular Sequence Data; D010795:Phthalic Acids / Q000737:chemistry / Q000378:metabolism*; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology; D014865:Waste Disposal, Fluid
D004275:DNA, Ribosomal; D007220:Industrial Waste; D010795:Phthalic Acids; D012336:RNA, Ribosomal, 16S; D012722:Sewage; C059768:isophthalate; D007455:Iodine