Mucosal immunization with a replication-deficient adenovirus vector expressing murine cytomegalovirus glycoprotein B induces mucosal and systemic immunity.
The murine cytomegalovirus (MCMV) glycoprotein B (gB) gene was expressed in an adenovirus replication-deficient vector. This virus, designated Ad-gB, was used to immunize BALB/c and B6 mice by the intranasal (i.n.) route to induce an immune response. Following primary immunization, antibody was detected in serum of 100% of vaccinees, as well as the bronchoalveolar lavage, fecal suspensions and vaginal washings. The viral titer of lung and salivary gland of vaccinees 10 days after intranasal challenge with MCMV at 10(5) or 10(3)plaque forming units (PFU) were significantly reduced compared to controls. Re-exposure of vaccinees to Ad-gB 30 days after primary immunization induced a remarkable boost of serum and mucosal antibody responses and further reduction of MCMV titers in the lung and salivary glands. The ability to induce both a systemic and mucosal immune response to a specific gene product may be important in reducing horizontal transmission of CMV infections across mucosal surfaces and in altering host immunity to CMV.
Shanley
John D
JD
Department of Medicine, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030, USA. jshanley@nso1.uchc.edu
Wu
Carol A
CA
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001483:Base Sequence; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D003586:Cytomegalovirus Infections / Q000276:immunology* / Q000517:prevention & control; D017931:DNA Primers; D005260:Female; D018928:Immunity, Mucosal; D007141:Immunoglobulin Fc Fragments / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012093:Replicon / Q000235:genetics*; D013997:Time Factors; D014759:Viral Envelope Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000627:therapeutic use*; D014779:Virus Replication / Q000235:genetics*
D000914:Antibodies, Viral; D017931:DNA Primers; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C040495:glycoprotein B, Simplexvirus