[Mechanisms of the alkaloid aurantioclavine excretion and uptake during Penicillium nalgiovense VKM F-229 growth].
The biphasic dynamics of the alkaloid aurantioclavine in the culture liquid of Penicillium nalgiovense VKM F-229 is shown to be due to the diauxic growth of the fungus on two carbon sources, succinate and mannitol. In the phase of active growth on succinate, the fungus synthesizes aurantioclavine and excretes it into the medium in an energy-independent manner, as a result of which the concentration of the alkaloid in the culture liquid rises. During the phase of metabolic adaptation to the other carbon source, mannitol, the concentration of aurantioclavine in the culture liquid falls, probably due to the energy-dependent uptake of the alkaloid by fungal cells. The reversible excretion of aurantioclavine in P. nalgiovense indicates that these are regulated processes and depend on the growth parameters and the physiological state of the fungus.
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, Pushchino, Moscow Oblast, 142290 Russia.
V P
VP
Zhelifonova
T V
TV
Kulakovskaia
A G
AG
Kozlovskiĭ
D001692:Biological Transport; D003470:Culture Media / Q000737:chemistry; D004876:Ergot Alkaloids / Q000096:biosynthesis / Q000378:metabolism*; D008353:Mannitol; D010407:Penicillium / Q000254:growth & development / Q000378:metabolism*; D019802:Succinic Acid
D003470:Culture Media; D004876:Ergot Alkaloids; D008353:Mannitol; D019802:Succinic Acid