Distribution of monoclonal antiferritin antibody in Kaposi's sarcoma, Hodgkin's disease, and hepatocellular carcinoma.
The immunotherapeutic treatment of cancers using antibodies (naked or conjugated to a drug, toxin, or radionuclide) relies upon the preferential expression of a targeted antigen on the cancer cell compared to normal tissues. Polyclonal antiferritin antisera have shown selective distribution and therapeutic efficacy when radiolabeled in Hodgkin's disease and hepatoma. In this immunohistochemical study, we investigated the distribution of ferritin in tumors from 6 patients with Kaposi's sarcoma, 12 patients with Hodkgin's disease, and 9 patients with hepatoma, as well as in selected normal tissues. We found that the monoclonal antiferritin antibody binds primarily to histiocytes in samples from Kaposi's sarcoma and Hodgkin's disease. One hepatocellular carcinoma showed diffuse cytoplasmic staining with ferritin. Deposition of the monoclonal antibody was not detectable in the remaining hepatocellular carcinoma samples.
Yuen
Alan R
AR
Departments of Medicine, Pathology, and Radiation Oncology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Higgins
John P
JP
Baker
Robin
R
Kamel
Onsi W
OW
Warnke
Roger A
RA
Knox
Susan J
SJ
D000911:Antibodies, Monoclonal / Q000276:immunology; D006528:Carcinoma, Hepatocellular / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D005293:Ferritins / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D015658:HIV Infections / Q000150:complications; D006689:Hodgkin Disease / Q000378:metabolism* / Q000473:pathology; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D012514:Sarcoma, Kaposi / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D012878:Skin Neoplasms / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology
D000911:Antibodies, Monoclonal; D005293:Ferritins