[Analysis of imazalil and its major metabolite in citrus fruits by GC-FTD].
A simplified simultaneous analytical method of imazalil (IZ) and its major metabolite, alpha-(2,4-dichlorophenyl)-1H-imidazole-1-ethanol (IZM), in citrus fruits was developed, and commodities samples were investigated. A homogenate of citrus fruits was extracted with ethyl acetate under basic conditions. The crude extract was partitioned between 0.025 mol/L of sulfuric acid and ethyl acetate. The analytes were extracted from the aqueous fraction under basic conditions with ethyl acetate. The extract solution was purified with an ENVI-Carb cartridge, and then analyzed by GC-FTD and GC/MS. Recoveries of IZ and IZM added to grapefruit at the level of 0.05 microgram/g were 90.0 and 108.7%, and those in the case of lemon were 100.4 and 93.0%, respectively. The detection limits were 0.01 microgram/g in samples. By this method, IZ and IZM were analyzed in 46 citrus fruits on the market and were detected simultaneously in some samples.
Kimura
Naoko
N
Tokyo Metropolitan Research Laboratory of Public Health: 3-24-1, Hyakunin-cho, Shinjuku-ku, Tokyo 169-0073, Japan.
Nagayama
Toshihiro
T
Takano
Ichiro
I
Kobayashi
Maki
M
Tamura
Yasuhiro
Y
Tateishi
Yukinari
Y
Kitayama
Kyoko
K
Saito
Kazuo
K
D002849:Chromatography, Gas; D002957:Citrus / Q000737:chemistry*; D005410:Flame Ionization; D007093:Imidazoles / Q000032:analysis* / Q000378:metabolism*; D010573:Pesticide Residues / Q000032:analysis*
D007093:Imidazoles; D010573:Pesticide Residues; C017435:enilconazole