Protein nanostructures formed via direct-write dip-pen nanolithography.
We report a tip modification strategy coupled with dip-pen nanolithography for depositing proteins on a surface. This methodology offers patterning capabilities on the scale of 45 nm to many micrometers length. The biorecognition properties of nanofeatures composed of immunoglobulin-gamma (IgG) were confirmed by reacting the array with gold nanoparticles coated with anti-IgG. Furthermore, a two component nanoarray was fabricated in direct-write fashion by DPN, and the biorecognition properties of this structure were investigated by AFM without biodiagnostic labels.
Department of Chemistry and Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208, USA.
Ki-Bum
KB
Lee
Jung-Hyurk
JH
Lim
Chad A
CA
Mirkin
D004247:DNA / Q000032:analysis / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D011506:Proteins / Q000032:analysis* / Q000737:chemistry
D011506:Proteins; D004247:DNA