Determination of aluminum in environmental and biological samples by reversed-phase high-performance liquid chromatography via pre-column complexation with morin.
Morin was used as a pre-column reagent for the determination of aluminum by RP-HPLC with fluorescence detection. This method has been successfully applied to direct determination of trace Al in environmental and biological samples. The response was linear from 6 x 10(-9) to 6 x 10(-5) M with a detection limit of 2 x 10(-9) M. In addition, the different Al complexes with morin were separated by the proposed HPLC procedure and their coordination ratios were depicted by molar-ratio method. The results showed that 1:1 and 2:1 Al-morin complexes formed.
State Key Laboratory of Coordination Chemistry, Department of Chemistry and Center of Materials Analysis, Nanjing University, 22 Hankou Road, Nanjing 210093, China.
Hong-Zhen
HZ
Lian
Yu-Fen
YF
Kang
Arkin
A
Yasin
Shu-Ping
SP
Bi
Da-Lin
DL
Shao
Yi-Jun
YJ
Chen
Le-Mei
LM
Dai
Li-Ching
LC
Tian
D000535:Aluminum / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004785:Environmental Pollutants / Q000032:analysis*; D005419:Flavonoids / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D047309:Flavones
D000535:Aluminum; D004785:Environmental Pollutants; D005419:Flavonoids; C008548:morin; D047309:Flavones