Mannosylerythritol lipids, yeast glycolipid biosurfactants, are potential affinity ligand materials for human immunoglobulin G.
Three mannosylerythritol lipids (MEL-A, -B, and -C), yeast glycolipid biosurfactants, were independently attached to poly (2-hydroxyethyl methacrylate) beads (PHEMA), and the three obtained MEL-PHEMA composites were examined for their binding affinity to human immunoglobulin G (HIgG). Of the three composites, the composite bearing MEL-A exhibited the highest binding capacity for HIgG. The binding amount of HIgG increased with increased applied concentration, reaching 106 mg HIgG (per g of composite), with a binding yield of 81%. Interestingly, the protein binding to the composite appeared to follow two different modes (Langmuir type and Freundlich type) depending on the applied concentration. The binding amount of human serum albumin to the composite was much smaller than that of HIgG. The bound human serum albumin, however, had minimal effect on the subsequent binding of HIgG, indicating that the two proteins have different binding sites onto the composite. More significantly, the bound HIgG was efficiently recovered under significantly mild elution conditions: Approximately 90% of the protein was eluted from the composite with phosphate buffer at pH 7. These results indicate that the glycolipid biosurfactant may have great potential as an affinity ligand material for HIgG.
Research Institute for Green Technology, National Institute of Advanced Industrial Science and Technology, Central 5-2, Higashi 1-1, Tsukuba, Ibaraki 305-8565, Japan.
Jae Hong
JH
Im
Hiroshi
H
Yanagishita
Toru
T
Ikegami
Yu-Ichiro
Y
Takeyama
Yasushi
Y
Idemoto
Nobuyuki
N
Koura
Dai
D
Kitamoto
D001665:Binding Sites; D006017:Glycolipids / Q000737:chemistry*; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry*; D007700:Kinetics; D008024:Ligands; D008968:Molecular Conformation; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry*
D006017:Glycolipids; D007074:Immunoglobulin G; D008024:Ligands; D012709:Serum Albumin; D013501:Surface-Active Agents; C439671:mannosylerythritol lipid