Protective effect of a polyherbal formulation (Immu-21) against cyclophosphamide-induced mutagenicity in mice.
The object was to evaluate the effects of a polyherbal formulation, Immu-21, against cyclophosphamide (CP)-induced chromosomal aberrations (CA) and micronuclei (MN) in mice. CP alone (40 mg/kg, i.p.) produced classical as well as non-classical chromosomal aberrations in mice, and the incidence of CA was significantly more in the CP treated group when compared with that of the control group. Immu-21, which contains extracts of Ocimum sanctum, Withania somnifera, Emblica officinalis and Tinospora cordifolia, was given at 100 mg/kg, daily, over 7 days, and 30 mg/kg daily over 14 days and inhibited both CP-induced classical and non-classical chromosomal aberrations ( approximately 40%-60% of control). A significant increase in MN was also observed in bone marrow erythrocytes of mice treated with CP, and pretreatment with Immu-21 also significantly reduced these. Cytotoxicity was evaluated by estimating the ratio of polychromatic erythrocytes (PCEs) to normochromatic erythrocytes (NCEs). The present results indicate that chronic treatment with Immu-21 prevented CP-induced genotoxicity in mice.
Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research, Sector-67, S A S Nagar, Punjab 160 062, India.
G B
GB
Jena
Kumar V S
KV
Nemmani
C L
CL
Kaul
P
P
Ramarao
D000818:Animals; D016587:Antimutagenic Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D001854:Bone Marrow Cells / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D002869:Chromosome Aberrations / Q000139:chemically induced / Q000187:drug effects*; D003520:Cyclophosphamide; D004912:Erythrocytes / Q000187:drug effects; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D031339:Ocimum; D029842:Phyllanthus emblica; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D018517:Plant Roots; D018547:Plant Stems; D031610:Tinospora; D032341:Withania
D016587:Antimutagenic Agents; D010936:Plant Extracts; C475842:polyherbal formulation Immu-21; D003520:Cyclophosphamide