In vitro action of combinations of selected antimicrobial agents and adult bovine articular cartilage (sesamoid bone).
Anatomically intact articular cartilage in form of sesamoid bones from metacarpophalangeal joints of 2-year-old cows was tested for its influence on the microbicidal effect of the iodophore Betaisodona, the bispyridinamine Octenisept, and the biguanide Lavasept. Comparisons were carried out in Ham's F12 medium with and without 0.2% bovine serum albumin as organic matter loading. The expected abolition of the microbicidal effect of these antiseptics against the test organisms Escherichia coli or Staphylococcus aureus in the presence of sesamoid bone was not evident. Furthermore, sesamoid bone alone demonstrated antibacterial activity against Staphylococcus aureus, which may involve adherence of bacteria to surface constituents of articular cartilage. Final concentrations of 2.5-5% Betaisodona, 5% Octenisept as well as 0.025% Lavasept are effective in killing of 10(8)-10(9) cfu/ml Escherichia coli or Staphylococcus aureus in the presence of sesamoid bone without the reduction of antimicrobial activity expected from binding to CS, which has previously been demonstrated for CS in solution.
Institute of Hygiene and Environmental Medicine, University of Greifswald, Hainstr. 26, D-17487 Greifswald, Germany.
Gerald
G
Müller
Axel
A
Kramer
D000818:Animals; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage*; D001645:Biguanides; D002358:Cartilage, Articular / Q000166:cytology* / Q000382:microbiology; D002417:Cattle; D015169:Colony Count, Microbial; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D005260:Female; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests; D011206:Povidone-Iodine / Q000008:administration & dosage; D012716:Sesamoid Bones; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development
D000891:Anti-Infective Agents, Local; D001645:Biguanides; D004338:Drug Combinations; D011206:Povidone-Iodine