Synthesis of protein-nucleic acid conjugates by expressed protein ligation.
The synthesis of covalent conjugates of proteins and polyamide nucleic acids (PNA) is accomplished by expressed protein ligation of intein-fusion proteins and a PNA-cysteine conjugate.
Lovrinovic
Marina
M
Universität Dortmund, Fachbereich Chemie, Biologisch-Chemische Mikrostrukturtechnik, Otto-Hahn Str. 6, D-44227 Dortmund, Germany.
Seidel
Ralf
R
Wacker
Ron
R
Schroeder
Hendrik
H
Seitz
Oliver
O
Engelhard
Martin
M
Goody
Roger S
RS
Niemeyer
Christof M
CM
D000596:Amino Acids / Q000737:chemistry; D001483:Base Sequence; D003545:Cysteine / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D004926:Escherichia coli / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D009696:Nucleic Acids / Q000737:chemistry* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis* / Q000378:metabolism; D019154:Protein Splicing; D011506:Proteins / Q000138:chemical synthesis* / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
D000596:Amino Acids; D009696:Nucleic Acids; D020135:Peptide Nucleic Acids; D011506:Proteins; D011993:Recombinant Fusion Proteins; D004247:DNA; D003545:Cysteine