[Isolation, culturation and adipogenisis committed differentiation of adult human mesenchymal stem cell].
To isolate MSCs from adult human bone marrow cells and to induce them into adipocytes.



MSCs were isolated from adult human bone marrow aspirated by Percoll and expanded in L-DMEM. The surface antigen of MSCs, CD14, CD34, CD45, CD44, VLA-1, HLA-DR and cell cycle were analysed on a FACScan flow cytometer. MSCs were cultured in adipogenisis inducing medium including insulin, 1-methyl-3-isobutylxanthine, indomethine and dexamethasone for 7 days and stained with Oil Red O.



MSCs grew as adherent cells and expanded more than 10 passages. They were positive for CD44 and negative for CD14, CD34, CD45, HLA-DR. The expression of VLA-1 was weak. After 7 days of adipocyte inducing, about 85%of the cells displayed accumulation of lipid vacuoles, as detected by Red Oil O.



MSCs isolated and cultured from adult human bone marrow can be induced to adipogenisis committed differentiation.
The Second Affiliated Hospital, College of Medical Sciences, Zhejiang University, Hangzhou 310009, China.
Hang-hui
HH
Cen
Chun-mao
CM
Han
Ping-ping
PP
Lai
Qing-Hua
QH
Lu
D017667:Adipocytes / Q000502:physiology*; D000328:Adult; D018952:Antigens, CD34 / Q000032:analysis; D001854:Bone Marrow Cells / Q000502:physiology*; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D018960:Hyaluronan Receptors / Q000032:analysis; D018950:Lipopolysaccharide Receptors / Q000032:analysis; D013234:Stem Cells / Q000502:physiology*
D018952:Antigens, CD34; D006684:HLA-DR Antigens; D018960:Hyaluronan Receptors; D018950:Lipopolysaccharide Receptors