Carbon and nitrogen source nutrition of fumagillin biosynthesis by Aspergillus fumigatus.
The carbon and nitrogen source requirements of Aspergillus fumigatus NRRL 2436 for growth and production of the angiogenesis inhibitor fumagillin were studied in chemically defined media. Both carbon and nitrogen sources strongly influenced fumagillin formation. Two out of 29 carbon sources tested interfered with fumagillin biosynthesis. The best combination of two carbon sources was 30 g L(-1) xylan and 50 g L(-1) mannose. Of fifteen nitrogen sources tested, three ammonium salts (chloride, sulfate, and dibasic phosphate) failed to support fumagillin formation, presumably due to the low pH which developed. The dosage-response study of the best nitrogen source, L-glutamic acid, revealed that 9 g L(-1) was optimal. Volumetric production of fumagillin was increased by 15-fold over that in the starting (Peterson-Goldstein) medium as a result of these findings.
Yang
Wen
W
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Kim
Wan-Seop
WS
Fang
Aiqi
A
Demain
Arnold L
AL
D001232:Aspergillus fumigatus / Q000254:growth & development / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D003510:Cyclohexanes; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D008358:Mannose / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism*; D012717:Sesquiterpenes
D003470:Culture Media; D003510:Cyclohexanes; D005231:Fatty Acids, Unsaturated; D012717:Sesquiterpenes; D002244:Carbon; C026211:fumagillin; D009584:Nitrogen; D008358:Mannose