Comparison of lateral-flow immunoassay and enzyme immunoassay with viral culture for rapid detection of influenza virus in nasal wash specimens from children.
The performance of two commercially available rapid test kits for influenza virus detection was compared to that of viral culture by using 356 nasal wash specimens collected during the 2001 to 2002 influenza season. Overall, the two rapid tests were easy to perform and showed comparable sensitivities (70.4 and 72.2%) and specificities (97.7 and 98.3%); for both test kit groups, most of the specimens that yielded false-negative results were found to be growing influenza B virus.
Department of Pediatrics, Baylor College of Medicine, Houston, Texas 77030, USA.
Andreea C
AC
Cazacu
Jewel
J
Greer
Moe
M
Taherivand
Gail J
GJ
Demmler
D000956:Antigens, Viral / Q000032:analysis; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000706:statistics & numerical data; D005188:False Negative Reactions; D006801:Humans; D007118:Immunoassay / Q000379:methods* / Q000706:statistics & numerical data; D009980:Influenza A virus / Q000276:immunology / Q000302:isolation & purification*; D009981:Influenza B virus / Q000276:immunology / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis / Q000821:virology; D009297:Nasal Mucosa / Q000821:virology; D012680:Sensitivity and Specificity; D014776:Virus Cultivation / Q000379:methods / Q000706:statistics & numerical data
D000956:Antigens, Viral