Reduced adhesion of blood cells to biodegradable polymers by introducing phosphorylcholine moieties.
Aliphatic polyesters are believed to be good biocompatible polymers for tissue engineering because of their biodegradability and nontoxicity of the degradated products. However, it is necessary to reduce the nonspecific protein adsorption for the application of biodegradable polymers to drug delivery systems or antiadhesive membranes. We hypothesized that novel biodegradable polymers could be synthesized by introducing phosphorylcholine moieties into aliphatic polyesters. The L-lactide was polymerized in the presence of L-alpha-glycelophosphorylcholine (LGPC) using stannous octate as the catalyst. The molecular weight and crystallinity of poly(L-lactide) (PLLA)-based phospholipid polymers (PLLA-PC) decreased with an increase in the composition of the LGPC unit in the PLLA-PC. The hydrolysis of the PLLA-PC was evaluated by soaking the polymer membranes in a phosphate buffer solution. The rate of weight loss was increased with increasing the LGPC units in PLLA-PC. The surface analysis of the membranes using an X-ray photoelectron microscope showed the composition of phosphorylcoline groups on the surface. The amount of adsorbed protein and adherent blood cell on the polymer surface was decreased with introducing LGPC unit. PLLA-PC is a promising biodegradable polymer having blood compatibility and antiadhesive property.
Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-surugadai, Chiyoda-ku, Tokyo 101-0062, Japan. yasu.org@tmd.ac.jp
Yasuhiko
Y
Iwasaki
Yoko
Y
Tojo
Tomoyuki
T
Kurosaki
Nobuo
N
Nakabayashi
D000327:Adsorption; D001672:Biocompatible Materials; D001773:Blood Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D001798:Blood Proteins / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D007962:Leukocytes / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D010767:Phosphorylcholine / Q000494:pharmacology*; D011091:Polyesters / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
D001672:Biocompatible Materials; D001798:Blood Proteins; D011091:Polyesters; D010767:Phosphorylcholine; C033616:poly(lactide)