Decorin modulates matrix mineralization in vitro.
Decorin (DCN), a member of small leucine-rich proteoglycans, is known to modulate collagen fibrillogenesis. In order to investigate the potential roles of DCN in collagen matrix mineralization, several stable osteoblastic cell clones expressing higher (sense-DCN, S-DCN) and lower (antisense-DCN, As-DCN) levels of DCN were generated and the mineralized nodules formed by these clones were characterized. In comparison with control cells, the onset of mineralization by S-DCN clones was significantly delayed; whereas it was markedly accelerated and the number of mineralized nodules was significantly increased in As-DCN clones. The timing of mineralization was inversely correlated with the level of DCN synthesis. In these clones, the patterns of cell proliferation and differentiation appeared unaffected. These results suggest that DCN may act as an inhibitor of collagen matrix mineralization, thus modulating the timing of matrix mineralization.
CB#7455, Dental Research Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7455, USA.
Yoshiyuki
Y
Mochida
Wagner R
WR
Duarte
Hideki
H
Tanzawa
Eleftherios P
EP
Paschalis
Mitsuo
M
Yamauchi
D000818:Animals; D002113:Calcification, Physiologic; D002455:Cell Division; D002999:Clone Cells; D003094:Collagen; D058575:Decorin; D005109:Extracellular Matrix; D016326:Extracellular Matrix Proteins; D007700:Kinetics; D051379:Mice; D009363:Neoplasm Proteins; D016376:Oligonucleotides, Antisense; D010006:Osteoblasts / Q000502:physiology; D011509:Proteoglycans / Q000235:genetics / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism
C546879:Dcn protein, mouse; D058575:Decorin; D016326:Extracellular Matrix Proteins; D009363:Neoplasm Proteins; D016376:Oligonucleotides, Antisense; D011509:Proteoglycans; D014157:Transcription Factors; D003094:Collagen