Passive length-tensile properties of extraocular muscles under botulinum toxin type C.
OBJECTIVE
Passive length-tension of the extraocular muscle was measured after injection of botulinum neurotoxin type C (BoNT-C) to evaluate its chemo-denervation effect.


METHODS
BoNT-C was injected unilaterally at 5.0 or 2.5 units in the superior rectus muscle of the albino rabbit. The muscle was separated several times at its original insertion between 3 days to 8 weeks after injection, and the passive length-tension produced by stretching the muscle from its physiologic length was measured. The length-tension curve was analyzed, and the passive load was determined from early components. The compliance was determined by approximating the entire ascending curve by an exponential function.


RESULTS
In the 5.0-unit group, the passive load increased significantly 2 weeks after injection but decreased to the level of the control group after 4 weeks and remained at that level until after 8 weeks after injection. In the 2.5-unit group, also, there were changes similar to those in the 5.0-unit group, but the changes were not significant compared to the control group. No significant change was observed in the compliance in either group.


CONCLUSIONS
Persistent chemo-denervation effect was not observed in passive length-tension at the doses of BoNT-C used in this study. The increase in the passive load soon after injection was suggested to have been caused by the same mechanism as BoNT-A.
Furuse
Takashi
T
Department of Ophthalmology, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan.
Hasebe
Satoshi
S
Ohtsuki
Hiroshi
H
Oguma
Keiji
K
D000818:Animals; D001905:Botulinum Toxins / Q000494:pharmacology*; D003187:Compliance; D007267:Injections; D009119:Muscle Contraction / Q000502:physiology; D009801:Oculomotor Muscles / Q000187:drug effects / Q000502:physiology*; D011817:Rabbits; D013563:Sympathectomy, Chemical; D013718:Tensile Strength
D001905:Botulinum Toxins; C096324:botulinum toxin type C