Expression of the yeast calcineurin subunits CNA1 and CNA2 during growth and hyper-osmotic stress.
CNA1 and CNA2 encode isoforms of the catalytic subunit of calcineurin, a Ser/Thr-specific phosphoprotein phosphatase regulated by Ca(2+)/calmodulin. The relative abundance of both transcripts was evaluated during growth of Saccharomyces cerevisiae in glucose by reverse-transcription polymerase chain reaction using PDA1 mRNA as a novel internal standard. CNA1 and CNA2 were concomitantly transcribed with different average expression ratios at the exponential and stationary growth phases and both showed a remarkable drop in the expression at diauxie. Prolonged hyper-osmotic shock resulted in a moderate induction of CNA1, whereas CNA2 expression was not affected.
Del Aguila
Eduardo Mere
EM
Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Centro de Tecnologia (CT), Bloco A - Lab. 545, 21949-900 Cidade Universitária, Rio de Janeiro, Brazil.
Silva
Joab Trajano
JT
Paschoalin
Vânia Margaret Flosi
VM
D019703:Calcineurin / Q000096:biosynthesis; D002135:Calcium-Binding Proteins; D015966:Gene Expression Regulation, Fungal; D006360:Heat-Shock Proteins; D009995:Osmosis / Q000502:physiology*; D010749:Phosphoprotein Phosphatases / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins
D002135:Calcium-Binding Proteins; D006360:Heat-Shock Proteins; D029701:Saccharomyces cerevisiae Proteins; C070394:CMP2 protein, S cerevisiae; C070393:CNA1 protein, S cerevisiae; D019703:Calcineurin; D010749:Phosphoprotein Phosphatases