[Protective effect of melatonin and vitamin E against prooxidative action of iron ions and static magnetic field].
The purpose of this study was to examine the effect of melatonin and vitamin E (trolox) on the level of lipid peroxidation in rat blood lymphocytes after in vitro (3 h) exposure to iron ions and/or 7mT static magnetic field (SMF). The lipid peroxidation process was chosen as a marker of free radical mechanism of SMF in cells. The cells were supplemented with (0.5 mM) melatonin or (0.1 mM) vitamin E (trolox) in preincubation. During SMF exposure in Helmholtz coils some samples were treated with ferrous chloride (10 mg/ml or 20 mg/ml), while the rest served as controls. There is a significant increase in the amount of lipid peroxidation end-products (4-HNE + MDA) in rat lymphocytes after simultaneous exposure to 7 mT SMF and iron ions (versus control samples and those exposed to SMF alone). Instead, when the cells were treated with melatonin or trolox and then exposed to iron ions and 7 mT SMF, the level of lipid peroxidation was significantly reduced. The results also indicated that melatonin is less effective than vitamin E (trolox) in inhibiting lipid peroxidation under the experimental conditions used.
Zakładu Toksykologii, Uniwersytetu Medycznego w łodzi.
Jolanta
J
Jajte
Marek
M
Zmyślony
Elzbieta
E
Rajkowska
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects / Q000528:radiation effects; D002470:Cell Survival; D004305:Dose-Response Relationship, Drug; D004574:Electromagnetic Fields / Q000009:adverse effects*; D005296:Ferrous Compounds / Q000633:toxicity*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D066298:In Vitro Techniques; D007501:Iron / Q000633:toxicity; D015227:Lipid Peroxidation / Q000187:drug effects* / Q000528:radiation effects; D008214:Lymphocytes / Q000187:drug effects* / Q000528:radiation effects; D008297:Male; D008550:Melatonin / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D014810:Vitamin E / Q000494:pharmacology*
D000975:Antioxidants; D005296:Ferrous Compounds; D016166:Free Radical Scavengers; D014810:Vitamin E; D007501:Iron; D008550:Melatonin