A novel method of generating prostate cancer metastases from orthotopic implants.
BACKGROUND
Spontaneous metastasis following implantation at the orthotopic site is a highly desired feature in prostate cancer (CaP) models, since it would enable studies of mechanisms associated with tumor cell dissemination.


METHODS
LuCaP 23.8 and LuCaP 35, hormone-sensitive CaP xenografts that express PSA and the wild-type androgen receptor, were grown orthotopically in SCID mice. When tumor volumes reached approximately 250-500 mg, primary tumors were removed to allow micrometastases to grow.


RESULTS
Using this procedure we generated macroscopic metastases (>20 mg) in 71% (LuCaP 23.8) and 100% (LuCaP 35) of animals, respectively.


CONCLUSIONS
These models may be used to evaluate new treatment modalities and study mechanisms associated with development of metastases.
University of Washington, Department of Urology, Seattle, Washington 98195, USA. ecorey@u.washington.edu
Eva
E
Corey
Janna E
JE
Quinn
Robert L
RL
Vessella
D000818:Animals; D004195:Disease Models, Animal; D006801:Humans; D008113:Liver Neoplasms / Q000556:secondary; D008175:Lung Neoplasms / Q000556:secondary; D008297:Male; D051379:Mice; D016513:Mice, SCID; D009368:Neoplasm Transplantation / Q000379:methods*; D010190:Pancreatic Neoplasms / Q000556:secondary; D017430:Prostate-Specific Antigen / Q000032:analysis; D011471:Prostatic Neoplasms / Q000737:chemistry / Q000556:secondary*; D014183:Transplantation, Heterologous
D017430:Prostate-Specific Antigen