Maltosyl-erythritol, a major transglycosylation product of erythritol by Bacillus stearothermophilus maltogenic amylase.
This study was done to modify erythritol to change its physicochemical and sensory properties. Erythritol, a four-carbon sugar alcohol, was transglycosylated by Bacillus stearothermophilus maltogenic amylase with maltotriose as a donor molecule. The presence of various transglycosylation products of erythritol was confirmed by TLC and high performance ion exchange chromatography (HPIC). The major transfer product was purified by gel filtration chromatography on Bio-Gel P-2. Examination by LC-MS, matrix-assisted laser desorption ionisation-time of flight mass spectrometry (MALDI-TOF-MS), and 13C NMR showed that the major transfer product was maltosyl-erythritol. Results of 13C NMR of maltosyl-erythritol suggested that linkage was formed between the C1 carbon of glucose unit in maltose and either one of the two carbon atoms of the terminal hydroxyl groups of erythritol, so that a mixture of 1-O- and 4-O-alpha-maltosyl-erythritol was produced. The sweetness of maltosyl-erythritol was about 40% that of sucrose, and its negative sensory properties were less than those of erythritol.
Center for Agricultural Biomaterials, National Laboratory for Functional Food Carbohydrates, and Department of Food Science and Technology, School of Agricultural Biotechnology, Seoul National University, Suwon 441-744, Korea.
Jong-Won
JW
Yoon
Eun-Joo
EJ
Jeon
Il-Hun
IH
Jung
Mee-Jung
MJ
Min
Hye-Young
HY
Lee
Myo-Jeong
MJ
Kim
Jin-Sook
JS
Baek
Hee-Seob
HS
Lee
Cheon-Seok
CS
Park
Sangsuk
S
Oh
Kwan-Hwa
KH
Park
Tae-Wha
TW
Moon
D000704:Analysis of Variance; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004896:Erythritol / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D001411:Geobacillus stearothermophilus / Q000201:enzymology*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008320:Maltose / Q000031:analogs & derivatives* / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013549:Sweetening Agents / Q000737:chemistry; D013649:Taste
D013549:Sweetening Agents; D008320:Maltose; D006026:Glycoside Hydrolases; D004896:Erythritol