The polysaccharide fraction AIP1 from Artemisia iwayomogi suppresses apoptotic death of the mouse spleen cells in culture.
A polysaccharide fraction, AIP1, purified from Artemisia iwayomogi was shown to have immunomodulating and anti-tumor activities in mice. In order to determine how the AIP1 fraction exhibits the immunomodulating activity, the effect of the fraction on the apoptosis of mouse spleen cells was investigated. Treatment of the mouse spleen cells with the AIP1 fraction resulted in the suppression of apoptotic death and an extension of cell survival in culture, indicating that the fraction might modulate the death of spleen cells. Treatment of the mice with the AIP1 fraction in vivo also resulted in less apoptosis of the spleen cells, which indicates the physiological relevance of the anti-apoptosis effect of the fraction in vitro. A mouse gene array was used to determine the profile of the gene expression change showing a pattern of up- and down-regulated genes by the AIP1 treatment. This study provides preliminary information regarding the immunomodulatory mechanism of the AIP1 fraction.
Department of Microbiology, School of Biotechnology & Biomedical Science, Inje University, Gimhae, Gyungnam 621-749, Korea.
Jung Suk
JS
Hwang
Hye Kyung
HK
Chung
Eun Kyong
EK
Bae
Ah Young
AY
Lee
Hee Jung
HJ
Ji
Dong Woon
DW
Park
Hwa Jin
HJ
Jung
Chung Won
CW
Cho
Hyun Ju
HJ
Choi
Dong Seok
DS
Lee
Kang Ro
KR
Lee
Hyun Joo
HJ
Youn
D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics*; D019306:Artemisia; D001854:Bone Marrow Cells / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects*; D005260:Female; D015870:Gene Expression / Q000187:drug effects* / Q000235:genetics*; D017737:Macrophages, Peritoneal / Q000187:drug effects; D051379:Mice; D010936:Plant Extracts; D018515:Plant Leaves; D011134:Polysaccharides / Q000235:genetics* / Q000276:immunology / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology* / Q000187:drug effects*
D010936:Plant Extracts; D011134:Polysaccharides