Pancreatic exocrine secretion in steers infused postruminally with casein and cornstarch.
Our objective was to evaluate the effect of postruminal protein infusion on pancreatic exocrine secretions. One Holstein, two crossbred, and five Angus steers (305 +/- 5 kg) with pancreatic pouch-duodenal reentrant cannulas and abomasal infusion catheters were used in a replicated 4 x 4 Latin square. All steers were abomasally infused with 1,050 g/d of raw cornstarch with treatments of 0, 60, 120, or 180 g/d of sodium casein suspended in water to yield 6,000 g/d of infusate daily. Steers were limit-fed (1.5 x NEm; 12 equal portions daily) a 90% corn silage, 10% supplement diet formulated to contain 12.5% CP. Periods consisted of 3 d of adaptation to infusion, 7 d of full infusion, 1 d of collection, and 7 d of rest. Pancreatic juice was collected in 30-min fractions continuously for 6 h. Total juice secreted and the pH of individual fractions were recorded, a 10% subsample was retained to form a composite sample, and remaining fluid was returned to the duodenum. Juice composite samples were stored (-30 degrees C) until analyzed for total protein and activities of alpha-amylase, trypsin, and chymotrypsin. Casein infusion linearly increased alpha-amylase concentration (182 to 271 units/mL; P < 0.02; 17.5 to 24.6 units/mg of protein; P < 0.03) and secretion rate (26,847 to 41,894 units/h; P < 0.01). Total juice secretion (155 g/h), pH of pancreatic juice (8.13), secretion rate of protein (1,536 mg/h), and concentration of protein (10.2 mg/mL) in pancreatic secretions were not affected (P > 0.05) by casein infusion. Similarly, casein infusion did not change 0.05) trypsin and chymotrypsin concentrations (1,379 and 349 units/L or 0.134 and 0.033 units/mg of protein, respectively) or secretion rates (206 and 52 units/h, respectively). Abomasal infusion of protein with starch stimulated a greater pancreatic secretion of alpha-amylase activity into the intestine than infusion of starch alone.
Richards
C J
CJ
Department of Animal Sciences, University of Kentucky, Lexington 40546-0215, USA.
Swanson
K C
KC
Paton
S J
SJ
Harmon
D L
DL
Huntington
G B
GB
D000018:Abomasum / Q000378:metabolism / Q000502:physiology; D000818:Animals; D002364:Caseins / Q000008:administration & dosage / Q000378:metabolism*; D002404:Catheterization; D002417:Cattle / Q000378:metabolism* / Q000502:physiology; D002918:Chymotrypsin / Q000032:analysis / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004386:Duodenum / Q000378:metabolism / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D007262:Infusions, Intravenous / Q000662:veterinary; D008297:Male; D010179:Pancreas / Q000378:metabolism*; D010189:Pancreatic Juice / Q000737:chemistry* / Q000378:metabolism; D013213:Starch / Q000008:administration & dosage / Q000378:metabolism*; D014357:Trypsin / Q000032:analysis / Q000378:metabolism; D000516:alpha-Amylases / Q000032:analysis* / Q000378:metabolism
D002364:Caseins; D013213:Starch; D000516:alpha-Amylases; D002918:Chymotrypsin; D014357:Trypsin