Induction of broad and potent anti-human immunodeficiency virus immune responses in rhesus macaques by priming with a DNA vaccine and boosting with protein-adsorbed polylactide coglycolide microparticles.
Several vaccine technologies were evaluated for their abilities to induce anti-human immunodeficiency virus Gag immune responses in rhesus macaques. While no vaccine alone was able to induce broad and strong immune responses, these were achieved by priming with Gag DNA and boosting with Gag protein adsorbed to polylactide coglycolide microparticles. This regimen elicited strong antibodies, helper T cells, and cytotoxic T lymphocytes and thus holds promise as an effective vaccination scheme.
Vaccines Research, Chiron Corporation, Emeryville, California 94608, USA. Gillis_Otten@chiron.com
Gillis
G
Otten
Mary
M
Schaefer
Catherine
C
Greer
Maria
M
Calderon-Cacia
Doris
D
Coit
Jina
J
Kazzaz
Angelica
A
Medina-Selby
Mark
M
Selby
Manmohan
M
Singh
Mildred
M
Ugozzoli
Jan
J
zur Megede
Susan W
SW
Barnett
Derek
D
O'Hagan
John
J
Donnelly
Jeffrey
J
Ulmer
D016915:AIDS Vaccines / Q000008:administration & dosage / Q000276:immunology*; D000818:Animals; D015683:Gene Products, gag / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D015483:HIV Antibodies / Q000097:blood; D015658:HIV Infections / Q000276:immunology / Q000517:prevention & control; D006801:Humans; D007114:Immunization; D007117:Immunization, Secondary; D019344:Lactic Acid / Q000276:immunology*; D008213:Lymphocyte Activation; D008253:Macaca mulatta; D008863:Microspheres; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D013601:T-Lymphocytes / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*
D016915:AIDS Vaccines; D015683:Gene Products, gag; D015483:HIV Antibodies; D011108:Polymers; D019444:Vaccines, DNA; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid