[Study on the oxidative injury of ECV304 cell induced by homocysteine].
The mechanism of homocysteine-induced oxidative injury on ECV304 cell line, a spontaneously transformed immortal human endothelial cell line, was studied. ECV304 cells were incubated with different levels of Hcy (0.05-2.00 mmol/L) for 12 hours. The activity of major anti-oxidative enzymes and the generation of reactive oxidative species (ROS) were measured by chromatometry and fluorochromy. The results showed that Hcy could induce obvious oxidative injury on ECV304 even at low level (0.05-0.10 mmol/L). The level of MDA was increased and the active oxygen was generated in the cells. The activities of anti-oxidative enzymes (SOD and GSH-Px) except the activity of CAT were decreased in the cells. It was concluded that Hcy may induce oxidative injury on EVC304 cell line through increasing the levels of ROS and inhibiting the activities of anti-oxidative enzymes.
Gao
Jian
J
Zhongshan Hospital Fudan University, Shanghai 200032, China.
Xue
Anna
A
D002460:Cell Line; D004730:Endothelium, Vascular / Q000473:pathology*; D006710:Homocysteine / Q000633:toxicity*; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism*; D014471:Umbilical Veins / Q000473:pathology
D017382:Reactive Oxygen Species; D006710:Homocysteine; D013482:Superoxide Dismutase