CD103+ intraepithelial lymphocytes--a unique population in microsatellite unstable sporadic colorectal cancer.
Colorectal cancers with microsatellite instability (MSI) typically show increased numbers of intraepithelial lymphocytes (IEL) in comparison to microsatellite stable (MSS) cancers. The aim of this study was to determine the phenotype of this unique lymphocyte population in MSI and MSS colorectal cancers. Twenty-four individuals with sporadic colorectal cancer (17 MSI, 7 MSS) were included in this study. Intraepithelial and stromal lymphocytes were detected using immunohistochemistry with anti-CD8 and anti-CD103 antibodies, and two observers independently quantified the numbers of lymphocytes. CD103+ (alpha E beta 7+) IELs detected within tumour tissue co-expressed CD8+ while the stromal lymphocytes were phenotypically heterogeneous, with respect to CD8+ and CD103+ expression. MSI colorectal cancers harboured increased numbers of CD8+ CD103+ IELs, as well as CD8+ CD103- and CD8+ CD103+ stromal lymphocytes, when compared with MSS colorectal cancers. CD103+ IELs were found at 27-fold greater numbers in the tumour epithelium than in normal epithelium from the same patient (P = 0.001, Wilcoxon matched pairs test). From our findings, we have proposed a mechanism for the homing of these alpha E beta 7+ lymphocytes to tumour tissue in MSI and MSS colorectal cancers.
Quinn
E
E
Department of Medical Oncology, St Vincent's Hospital, School of Microbiology and Immunology, University of New South Wales, Australia.
Hawkins
N
N
Yip
Y L
YL
Suter
C
C
Ward
R
R
D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000378:metabolism*; D016827:CD8 Antigens / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000276:immunology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D039001:Integrin alpha Chains / Q000378:metabolism*; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology*; D008297:Male; D018895:Microsatellite Repeats; D010641:Phenotype
D015703:Antigens, CD; D016827:CD8 Antigens; D039001:Integrin alpha Chains; C091367:alpha E integrins