Synthetic propeptide inhibits mosquito midgut chitinase and blocks sporogonic development of malaria parasite.
Incessant transmission of the parasite by mosquitoes makes most attempts to control malaria fail. Blocking of parasite transmission by mosquitoes therefore is a rational strategy to combat the disease. Upon ingestion of blood meal mosquitoes secrete chitinase into the midgut. This mosquito chitinase is a zymogen which is activated by the removal of a propeptide from the N-terminal. Since the midgut peritrophic matrix acts as a physical barrier, the activated chitinase is likely to contribute to the further development of the malaria parasite in the mosquito. Earlier it has been shown that inhibiting chitinase activity in the mosquito midgut blocked sporogonic development of the malaria parasite. Since synthetic propeptides of several zymogens have been found to be potent inhibitors of their respective enzymes, we tested propeptide of mosquito midgut chitinase as an inhibitor and found that the propeptide almost completely inhibited the recombinant or purified native Anopheles gambiae chitinase. We also examined the effect of the inhibitory peptide on malaria parasite development. The result showed that the synthetic propeptide blocked the development of human malaria parasite Plasmodium falciparum in the African malaria vector An. gambiae and avian malaria parasite Plasmodium gallinaceum in Aedes aegypti mosquitoes. This study implies that the expression of inhibitory mosquito midgut chitinase propeptide in response to blood meal may alter the mosquito's vectorial capacity. This may lead to developing novel strategies for controlling the spread of malaria.
International Center for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, P.O. Box 10504, New Delhi 1100 67, India. raj@icgeb.res.in
Raj K
RK
Bhatnagar
Naresh
N
Arora
S
S
Sachidanand
Mohammed
M
Shahabuddin
David
D
Keister
Virendra S
VS
Chauhan
D000595:Amino Acid Sequence; D000818:Animals; D020134:Catalytic Domain; D002688:Chitinases / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D009033:Culicidae / Q000378:metabolism* / Q000469:parasitology; D004064:Digestive System / Q000378:metabolism; D006801:Humans; D019476:Insect Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism*; D010963:Plasmodium falciparum / Q000254:growth & development* / Q000502:physiology; D010964:Plasmodium gallinaceum / Q000254:growth & development / Q000502:physiology
D019476:Insect Proteins; D010455:Peptides; D002688:Chitinases