Treatment of adjuvant arthritis with granulocyte-colony stimulating factor and peptide derived from heat shock protein 65.
Adjuvant arthritis in Lewis rats is induced by the subcutaneous injection of Mycobacterium tuberculosis in mineral oil, and the predominant T cell immune reactivity is against the heat shock protein 65 derived peptide 176-190. We treated Lewis rats with human recombinant G-CSF followed by (i.v) administration of peptide 176-190 after induction of adjuvant arthritis (AA), and observed decreased disease severity, joint destruction, new bone formation and joint ankylosis. Treatment with G-CSF alone was also effective, but to a lesser extent. In addition, we found that splenocytes from rats treated with G-CSF had reduced antigen presenting capacity compared with splenocytes from vehicle treated rats. Primed lymph node cells from G-CSF plus peptide treated rats showed a marked reduction in proliferation and secretion of IFN-gamma after stimulation with the heat shock protein peptide in vitro as compared to controls.
Department of Medicine, Division of Immunology and Rheumatology, Stanford University School of Medicine, CCSR Bldg Rm 2215, CA 94305, USA.
Andrea
A
Brendolan
Masanori
M
Higuchi
Richard
R
Sibley
Samuel
S
Strober
D000595:Amino Acid Sequence; D000818:Animals; D017951:Antigen Presentation; D001169:Arthritis, Experimental / Q000175:diagnosis / Q000188:drug therapy* / Q000276:immunology; D001426:Bacterial Proteins; D039481:CD11b Antigen / Q000032:analysis; D002460:Cell Line; D018834:Chaperonin 60; D018833:Chaperonins / Q000737:chemistry; D004359:Drug Therapy, Combination; D016179:Granulocyte Colony-Stimulating Factor / Q000627:therapeutic use*; D007958:Leukocyte Count; D008213:Lymphocyte Activation; D008297:Male; D010455:Peptides / Q000627:therapeutic use; D011859:Radiography; D051381:Rats; D011917:Rats, Inbred Lew; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013640:Tarsal Joints / Q000000981:diagnostic imaging / Q000473:pathology
D001426:Bacterial Proteins; D039481:CD11b Antigen; D018834:Chaperonin 60; D010455:Peptides; C074119:heat-shock protein 65, Mycobacterium; D016179:Granulocyte Colony-Stimulating Factor; D018833:Chaperonins