A novel serine protease of the mammalian HtrA family is up-regulated in mouse uterus coinciding with placentation.
This paper characterizes a novel gene, previously identified as uniquely regulated at implantation in mouse uterus. We cloned its full mRNA sequence encoding a serine protease possessing an IGF-binding domain and named it pregnancy-related serine protease (PRSP). PRSP is structurally similar to mammalian HtrA1 (56% amino acid similarity). Northern analysis revealed that the expression of PRSP mRNA was low before pregnancy, but it was increased at implantation and markedly up-regulated post-implantation. In-situ hybridization localized low levels of mRNA expression to the epithelium and stroma during very early pregnancy, but high expression to the decidual cells on day 8.5, primarily at the mesometrial pole where the placenta was forming. By day 10.5, PRSP mRNA was detected in the placenta. We also cloned an alternatively spliced PRSP mRNA that is expressed at a very low level. We located PRSP gene on chromosome 5 and established its intron/exon structure, which unambiguously explains how the two mRNA variants are produced through alternative splicing. Based on PRSP protein domain structure and its unique expression during pregnancy, we propose that PRSP plays an important role in the formation/function of the placenta.
Nie
Gui-Ying
GY
Prince Henry's Institute of Medical Research, P.O.Box 5152, 246 Clayton Road, Clayton, Victoria 3168, Australia. guiying.nie@med.monash.edu.au
Li
Ying
Y
Minoura
Hiroyuki
H
Batten
Leigh
L
Ooi
Guck T
GT
Findlay
Jock K
JK
Salamonsen
Lois A
LA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005260:Female; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010929:Placentation / Q000502:physiology*; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation; D014599:Uterus / Q000201:enzymology*
D012333:RNA, Messenger; C474013:HTRA3 protein, human; C485358:PRSP protein, mouse; D012697:Serine Endopeptidases