Allele-specific differential expression of a common adiponectin gene polymorphism related to obesity.
Adiponectin gene polymorphisms have recently been reported to be associated with obesity, insulin sensitivity, and the risk of type 2 diabetes. We examined a T94G polymorphism of the adiponectin gene in 245 ostensibly normal nondiabetic subjects. The G allele frequency was lower among subjects with higher BMI (> or =27) than in those with lower BMI. BMI was inversely correlated with the dose of G allele. Multivariate linear regression analyses showed that the adiponectin genotypes were significantly related to BMI after adjusting for age and gender. The dose of the G allele was associated with a reduction of approximately 1.12 kg/m(2) in BMI. We further found that the relative mRNA levels of G allele were consistently higher than those of T allele in the omental adipose tissue from 21 heterozygous subjects. Finally, we observed that the expression levels of adiponectin affected insulin-stimulated glucose uptake in differentiated 3T3-L1 adipocytes. In conclusion, the allele-specific differential expression of this common polymorphism could be responsible for its biological effects observed in this and the other studies.
Yang
Wei-Shiung
WS
Division of Endocrinology and Metabolism, Department of Internal Medicine, National Taiwan University Hospital, and Graduate Institute of Clinical Medicine, National Taiwan University Medical College, Taipei, Taiwan.
Tsou
Pei-Ling
PL
Lee
Wei-Jei
WJ
Tseng
Da-Lun
DL
Chen
Chi-Ling
CL
Peng
Chi-Chung
CC
Lee
Kuan-Ching
KC
Chen
Mei-Ju
MJ
Huang
Chang-Jen
CJ
Tai
Tong-Yuan
TY
Chuang
Lee-Ming
LM
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism*; D052242:Adiponectin; D000328:Adult; D000483:Alleles; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D015992:Body Mass Index; D005260:Female; D015870:Gene Expression; D005951:Glucose Tolerance Test; D006801:Humans; D007328:Insulin / Q000494:pharmacology / Q000502:physiology; D007333:Insulin Resistance; D036341:Intercellular Signaling Peptides and Proteins; D008297:Male; D051379:Mice; D008875:Middle Aged; D009765:Obesity / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics* / Q000378:metabolism
D052242:Adiponectin; D001786:Blood Glucose; D007328:Insulin; D036341:Intercellular Signaling Peptides and Proteins; D011506:Proteins