[Effect of cadmium on apoptosis of spermatogenic cells of rat testis and the protection effect of zinc against it].
To study the effect of cadmium on the apoptosis of spermatogenic cells of rat testis and the protective effect of zinc against it. 24 Wistar male rats were divided into 3 groups (cadmium, cadmium and zinc, and control rats). Rats were injected with low-dose CdCl2 (2 mg/kgBW), zinc acetate(ZnAc, 15 mg/kgBW) before and after injected with low-dose CdCl2 and ZnAc(50 mg/kgBW). The control rats were injected with same-dose of 0.9% NaCl. After 7 days, the apoptosis of spermatogenic cells was studied with in situ nick translation(ISNT) technique. Compared with control group, the number of apoptosis spermategenic cells was obviously increased in cadmium group(P < 0.01, t = 3.87), but it was not significantly different in cadmium and zinc rats. Cadmium treatment could accelerate testis apoptosis. Zinc could proven it.
Department of Neurobiology, Chinese People's Armed Police Force Medical College, Tianjin 300162, China.
J
J
Li
J
J
Yi
C
C
Wang
P
P
Xu
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D019256:Cadmium Chloride / Q000633:toxicity*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013090:Spermatocytes / Q000166:cytology* / Q000187:drug effects; D013737:Testis / Q000166:cytology; D019345:Zinc Acetate / Q000494:pharmacology*
D019345:Zinc Acetate; D019256:Cadmium Chloride