PKR is not required for interferon-gamma inhibition of VSV replication in neurons.
In this report, the contribution of PKR to the IFN-gamma mediated inhibition of VSV replication in neurons was examined. IFN-gamma treatment of NB41A3 murine neuroblastoma cells resulted in the reduced expression of VSV protein during infection. PKR was found to be modestly upregulated in NB41A3 cells following IFN-gamma treatment. The phosphorylation state of PKR and its downstream target, eIF2alpha, were unaffected by either IFN-gamma or VSV infection. Inhibition of PKR through the use of 2-aminopurine or the expression of the Influenza A NS1 gene had no effect on the ability of IFN-gamma to inhibit the replication of VSV in vitro. These data indicate that endogenously expressed PKR is not required for the IFN-gamma mediated inhibition of VSV replication in NB41A3 neuroblastoma cells.
Chesler
David A
DA
Department of Biology, New York University, New York, New York 10003, USA.
Muñoz-Jordán
Jorge Luis
JL
Donelan
Nicola
N
García-Sastre
Adolfo
A
Reiss
Carol Shoshkes
CS
D000818:Animals; D007371:Interferon-gamma / Q000494:pharmacology*; D051379:Mice; D009474:Neurons / Q000821:virology*; D014407:Tumor Cells, Cultured; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects / Q000502:physiology*; D014764:Viral Proteins / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects*; D019892:eIF-2 Kinase / Q000378:metabolism*
D014764:Viral Proteins; D007371:Interferon-gamma; D019892:eIF-2 Kinase