Effect of menhaden fish oil supplementation and lipopolysaccharide exposure on nursery pigs. II. Effects on the immune axis when fed simple or complex diets containing no spray-dried plasma.
A trial using 64 weanling pigs (TR4 x PIC C22) was conducted to determine the effects of menhaden fish oil supplementation and diet complexity on performance and immune response of nursery pigs. Pigs (17 days and 6.27+/-1.16 kg) were weaned into a segregated early wean facility and given free access to a complex diet for 7 days post-weaning. At day 0 (day 7 post-weaning), pigs were blocked by weight and allotted to 64 pens. Treatments (Trt) were arranged as a 2 x 2 x 2 factorial arrangement. Main effects included diet (complex versus simple), oil (menhaden fish (MFO) versus corn (CO)), and immunogen (saline versus lipopolysaccharide (LPS)). Experimental diets contained 6% oil (6% CO or 5% MFO+1% CO) and were fed for 14 days. On day 12, i.v. injections of either LPS (150 microg/kg) or saline were given, followed by blood collection at 30 min intervals for 6h. After the immune challenge (day 14), pigs were placed onto a common corn-soybean meal fortified diet and growth performance was evaluated until termination of the study (day 28). Pigs were weighed and feed intakes recorded at 7, 14, and 28 days. Prior to immune challenge (day 12), there were differences in BW for pigs fed complex versus simple diets (P<0.01; 13.1 and 12.1 kg, respectively) and pigs fed CO versus MFO diets (P<0.05; 12.9 and 12.3 kg, respectively). During the challenge period, for pigs treated with LPS there was a Time x Immunogen x Oil effect (P<0.001) for serum cortisol with MFO fed pigs having lower serum cortisol as compared to CO fed pigs. Also, during the challenge period, for pigs treated with LPS there was a Time x Diet x Immunogen x Oil effect (P<0.001) for serum tumor necrosis factor-alpha (TNF-alpha) with pigs fed complex diets supplemented with CO having higher serum TNF-alpha as compared with pigs fed complex diets supplemented with MFO. At days 14 and 28, LPS-treated pigs had lower BW than saline injected controls (P<0.001 and 0.01, respectively). In addition, pigs fed simplified diets continued to have lower BW after challenge compared to pigs fed a complex diet. Interestingly, there were no differences (P>0.10) in BW after challenge in pigs fed MFO. This study suggests that MFO supplementation alters the immune response during LPS challenge and that simplified diets may compromise nursery performance.
Gaines
A M
AM
University of Missouri, Columbia, MO, USA.
Carroll
J A
JA
Yi
G F
GF
Allee
G L
GL
Zannelli
M E
ME
D000818:Animals; D001835:Body Weight; D002097:C-Reactive Protein / Q000032:analysis; D003314:Corn Oil / Q000008:administration & dosage; D004032:Diet; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage*; D005395:Fish Oils / Q000008:administration & dosage*; D006242:Haptoglobins / Q000032:analysis; D006854:Hydrocortisone / Q000097:blood; D007700:Kinetics; D008070:Lipopolysaccharides / Q000008:administration & dosage*; D010949:Plasma; D013025:Glycine max; D013552:Swine / Q000254:growth & development / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis; D014886:Weaning
D004042:Dietary Fats, Unsaturated; D005395:Fish Oils; D006242:Haptoglobins; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; C033266:Menhaden oil; D003314:Corn Oil; D002097:C-Reactive Protein; D006854:Hydrocortisone