Laser capture microdissection of cells from plant tissues.
Laser capture microdissection (LCM) is a technique by which individual cells can be harvested from tissue sections while they are viewed under the microscope, by tacking selected cells to an adhesive film with a laser beam. Harvested cells can provide DNA, RNA, and protein for the profiling of genomic characteristics, gene expression, and protein spectra from individual cell types. We have optimized LCM for a variety of plant tissues and species, permitting the harvesting of cells from paraffin sections that maintain histological detail. We show that RNA can be extracted from LCM-harvested plant cells in amount and quality that are sufficient for the comparison of RNAs among individual cell types. The linear amplification of LCM-captured RNA should permit the expression profiling of plant cell types.
Kerk
Nancy M
NM
Department of Molecular, Cellular and Developmental Biology, Yale University, P.O. Box 208104, New Haven, Connecticut 06520-8104, USA.
Ceserani
Teresa
T
Tausta
S Lorraine
SL
Sussex
Ian M
IM
Nelson
Timothy M
TM
D018744:DNA, Plant / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D016591:Histocytological Preparation Techniques / Q000295:instrumentation / Q000379:methods*; D007834:Lasers; D059828:Plant Cells; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D010944:Plants / Q000737:chemistry / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D012680:Sensitivity and Specificity; D016610:Tissue Embedding; D016707:Tissue Fixation
D018744:DNA, Plant; D010940:Plant Proteins; D018749:RNA, Plant