Biological activity of persimmon (Diospyros kaki) peel extracts.
Fractionated extracts of persimmon (Diospyros kaki) peels were studied for cytotoxic activity, multidrug resistance (MDR) reversal activity, anti-human immunodeficiency virus (HIV) activity and anti-Helicobacter pylori (H. pylori) activity. The potent cytotoxic activity against human oral squamous cell carcinoma cells (HSC-2) and human submandibular gland tumor (HSG) cells was found in the acetone fractions (A4 and A5) with IC(50) ranging from 21 to 59 micro g/mL. However, the cytotoxic activity was not correlated with the radical intensity of the fractions. Three 70% MeOH extract fractions (70M2-4) produced radical and efficiently scavenged the O(2)(-) produced by hypoxanthine and xanthine oxidase reaction. All of the fractions tested were not effective for anti-H. pylori and anti-HIV. Fractions H3 and H4 of hexane extract, and M2 and M3 of MeOH extract showed a remarkable MDR reversal activity comparable with that of (+/-)-verapamil (a positive control). These results indicate the therapeutic value of persimmon peel extracts as potential antitumor and MDR-reversing agents.
Faculty of Pharmaceutical Sciences, Josai University, Sakado, Saitama, Japan. kawasema@josai.ac.jp
Masami
M
Kawase
Noboru
N
Motohashi
Kazue
K
Satoh
Hiroshi
H
Sakagami
Hideki
H
Nakashima
Satoru
S
Tani
Yoshiaki
Y
Shirataki
Teruo
T
Kurihara
Gabriella
G
Spengler
Krisztina
K
Wolfard
Joseph
J
Molnár
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology / Q000627:therapeutic use; D002455:Cell Division / Q000187:drug effects; D029788:Diospyros; D018432:Drug Resistance, Multiple; D005638:Fruit / Q000737:chemistry; D015497:HIV-1 / Q000187:drug effects* / Q000254:growth & development; D016480:Helicobacter pylori / Q000187:drug effects* / Q000254:growth & development; D006801:Humans; D020128:Inhibitory Concentration 50; D019441:Plant Epidermis / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000821:virology
D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts