Intercalibration of chromatographic methods for auxino phytodrugs in Solanaceae.
Three chromatographic methods are considered for the determination in Solanaceae of auxino-similar phytodrugs, so called because their structure resembles an auxine plant hormone. The phytodrugs studied were: 2,4-dichlorophenoxyacetic acid, 2,4-dichlorophenoxypropionic acid, 2,4-dichlorophenoxybutyric acid, 2-methyl-4-chlorophenoxyacetic acid, 2,4,5-trichlorophenoxyacetic acid, naphthylacetic acid and 2-naphthyloxyacetic acid. Three chromatographic methods, respectively based on ion-interaction HPLC, GC-MS with intra-injector derivatisation and CC-MS with pre-injection derivatisation, were developed, optimised and validated. A comparative discussion of the advantages/disadvantages of the methods suggests a strategy for their preferential use, that is essentially a function of the matrix complexity.
Gennaro
M C
MC
Dipartimento di Scienze e Tecnologie Avanzate, Università del Piemonte Orientale A. Avogadro, Spalto Marengo 33, Alessandria 15100, Italy. gennaro@mfn.unipmn.it
Marengo
E
E
Gianotti
V
V
Angioi
S
S
Copeta
G
G
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007210:Indoleacetic Acids / Q000302:isolation & purification*; D010937:Plant Growth Regulators / Q000302:isolation & purification*; D015203:Reproducibility of Results; D019657:Solanaceae / Q000737:chemistry*
D007210:Indoleacetic Acids; D010937:Plant Growth Regulators