[Effects of pre-heating on the sensitivity of K562 cell to tumor necrosis factor-alpha].
In order to study the effects of pre-heating on the sensitivity of cells to tumor necrosis factor-alpha (TNF-alpha), the K562 cells were pre-heated at 40 degrees C for different times to induce heat shock protein 70 (HSP70). To observe the effects of TNF-alpha concentration on the sensitivity of pre-heated cells, cells were pre-heated for 60 min and treated with TNF-alpha (50, 100, 200 and 400 U/ml) for 16 hours, and the cells vitalities were detected by MTT. In order to observe the effect of pre-heating time on the sensitivity of cells to TNF-alpha, the pre-heated cells were treated with 100 U/ml TNF-alpha for 24 hours. The levels of HSP70 were tested by reversed transcription-PCR(RT-PCR). The results showed that HSP70 could be induced by pre-heating, and reached its top level after 120 mins, and the sensitivity of non-heated cells to TNF-alpha was lower than that of pre-heated cells. To 100 U/ml TNF-alpha, the sensitivity was lowest after the cells were pre-heated for 120 min. The results suggested that pre-heated could induce the expression of HSP70 and inhibit the sensitivity of cell to TNF-alpha, the mechanism may be involved with the overexpression of HSP70.
Department of Military Hygiene, Fourth Military Medical University, Xi-an 710032, China.
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Wang
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Zhao
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D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D006358:Hot Temperature; D006801:Humans; D020014:K562 Cells / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha