Detection of human papillomavirus-16 and HPV-18 DNA in normal, dysplastic, and malignant oral epithelium.
OBJECTIVE
Our aim was to clarify the association of human papillomavirus (HPV) with oral carcinogenesis, especially its early stage.


METHODS
Tissue specimens of normal mucosa, epithelial dysplasia, oral squamous cell carcinoma (OSCC), and OSCC cell lines were examined for the presence of HPV-16 and HPV-18 E6 DNA by means of the polymerase chain reaction test.


RESULTS
The detection rate of HPV-16 in epithelial dysplasia (31/51) was higher than that in normal mucosa (16/44) and in OSCC (30/86) and was statistically different from that in OSCC. The cases that progressed from epithelial dysplasia to carcinoma showed a significantly higher HPV-16 detection rate than the other cases in both epithelial dysplasia and OSCC. HPV-16 and HPV-18 were detected only at early passages of 2 of 10 OSCC cell lines.


CONCLUSIONS
These results strongly suggest that HPV-16 may be involved in the early stages of the development of some oral carcinomas.
Department of Oral and Maxillofacial Surgery, Graduate School of Biomedical Sciences, Hiroshima University, Kasumi 1-2-3, Minami-Ku, Hiroshima 734-8553, Japan. masaru@hiroshima-u.ac.jp
Masaru
M
Sugiyama
Ujjal Kumar
UK
Bhawal
Tamiko
T
Dohmen
Shigehiro
S
Ono
Miwa
M
Miyauchi
Takenori
T
Ishikawa
D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000556:secondary / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis*; D018450:Disease Progression; D004848:Epithelium / Q000821:virology; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000473:pathology / Q000821:virology*; D009062:Mouth Neoplasms / Q000821:virology*; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000821:virology; D011230:Precancerous Conditions / Q000821:virology; D018709:Statistics, Nonparametric; D014407:Tumor Cells, Cultured; D014412:Tumor Virus Infections / Q000821:virology
D004279:DNA, Viral