Carbon dioxide action on ethylene biosynthesis of preclimacteric and climacteric pear fruit.
Ethylene production in pear fruit was studied at 2 degrees C. Several observations showed that the inhibiting effect of CO2 on ethylene production did not operate only via the binding site of the ethylene binding protein. Ethylene production of freshly harvested pears was stimulated by 1-methylcyclopropene (1-MCP), but unaffected or inhibited by CO2 which points to different action sites for both molecules. In climacteric pears, where ethylene production was strongly inhibited by 1-MCP, a range of applied CO2 partial pressures was able to inhibit ethylene production further, to an extent similar to untreated pears. In the case of pears that had been stored for a period of 25 weeks, CO2 only had a clear effect after 1-MCP pretreatment. Respiration measurements showed that the effect of CO2 on ethylene production did not operate via an effect on respiration. Ethylene production models based on measurements of whole pears were used to study CO2 effects. Kinetic parameters derived from the models point to the conversion from ACC to ethylene by ACC oxidase as a possible action site for CO2 inhibition.
Agrotechnological Research Institute (ATO), PO Box 17, 6700 AA Wageningen, The Netherlands. J.P.deWild@ato.wag-ur.nl
Hans P J
HP
de Wild
Els C
EC
Otma
Herman W
HW
Peppelenbos
D000594:Amino Acid Oxidoreductases / Q000378:metabolism; D000598:Amino Acids, Cyclic / Q000378:metabolism; D002245:Carbon Dioxide / Q000494:pharmacology*; D019069:Cell Respiration / Q000187:drug effects / Q000502:physiology; D003521:Cyclopropanes / Q000494:pharmacology; D005030:Ethylenes / Q000096:biosynthesis*; D005638:Fruit / Q000187:drug effects / Q000378:metabolism*; D008954:Models, Biological; D010100:Oxygen / Q000378:metabolism*; D031989:Pyrus / Q000187:drug effects / Q000378:metabolism*
D000598:Amino Acids, Cyclic; D003521:Cyclopropanes; D005030:Ethylenes; D002245:Carbon Dioxide; C023863:1-aminocyclopropane-1-carboxylic acid; C036216:ethylene; D000594:Amino Acid Oxidoreductases; C059460:1-aminocyclopropane-1-carboxylic acid oxidase; C412563:1-methylcyclopropene; D010100:Oxygen