Characterization of a Saccharomyces cerevisiae mutant with pseudohyphae and cloning of a gene complementing the mutation.
Screening for morphological mutants of a haploid strain of Saccharomyces cerevisiae was done on the basis of their cell-shape on a solid medium containing isoamyl alcohol, which causes cell elongation, to obtain information on the morphogenesis. Mutant J19, which had pseudohyphae in liquid medium even in the absence of isoamyl alcohol, had many elongated cells. Few reports exist of haploid cells growing as pseudohyphae in liquid culture. Cell-wall analysis showed that J19 had ordinary amounts of alkali-insoluble glucan and chitin, but that isoamyl alcohol in the medium caused structural changes in the cell wall. Addition of a DNA fragment that included the wild-type SCL1 gene to J19 complemented its morphological phenotype. Sequencing of J19 SCL1 showed that the glycine at position 226 in the Scl1 protein had been replaced by asparatic acid, suggesting that this mutation in the protein, a subunit of proteasomes, may be involved in the morphological change.
Maneesri
Jaruwan
J
Department of Applied and Bioapplied Chemistry, Graduate School of Engineering, Osaka City University, Sugimoto 3-3-138, Sumiyoshi-ku, Osaka 558-8585, Japan.
Azuma
Masayuki
M
Torii
Satoshi
S
Igarashi
Koichi
K
Ooshima
Hiroshi
H
D002473:Cell Wall / Q000737:chemistry / Q000187:drug effects; D003001:Cloning, Molecular; D005800:Genes, Fungal / Q000235:genetics*; D005816:Genetic Complementation Test; D005936:Glucans / Q000737:chemistry; D008856:Microscopy, Fluorescence; D009024:Morphogenesis / Q000235:genetics; D009154:Mutation; D000439:Pentanols / Q000494:pharmacology; D010641:Phenotype; D010957:Plasmids / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development* / Q000648:ultrastructure; D017422:Sequence Analysis, DNA; D014170:Transformation, Genetic
D005936:Glucans; D000439:Pentanols; C029683:isopentyl alcohol