Protein-protein unbinding induced by force: single-molecule studies.
Experiments in which two specifically interacting protein molecules are dissociated by external force have yielded new insights into mechanisms involved in cell adhesion, leukocyte rolling, regulation of integrin activity, antigen-antibody interactions and other protein-mediated reactions contingent upon molecular recognition. Another important aspect of force-induced protein-protein unbinding studies is the new information being gleaned about the thermodynamics and kinetics of bond rupture.
Department of Cell & Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia 19104-6058, USA. weisel@cellbio.med.upenn.edu
John W
JW
Weisel
Henry
H
Shuman
Rustem I
RI
Litvinov
D001665:Binding Sites; D015815:Cell Adhesion Molecules / Q000737:chemistry*; D003198:Computer Simulation; D008832:Microchemistry / Q000379:methods*; D008958:Models, Molecular; D036103:Nanotechnology / Q000379:methods*; D010812:Physical Stimulation / Q000379:methods*; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D013314:Stress, Mechanical; D013329:Structure-Activity Relationship
D015815:Cell Adhesion Molecules; D011506:Proteins