Analysis of 148 kb of genomic DNA of Tetraodon nigroviridis covering an amylase gene family.
We have sequenced and analysed a 148 kb genomic region of Tetraodon nigroviridis, a teleost fish with a compact genome. Several genes were identified by comparison with genomic or transcript sequences of other species, informatic prediction and screening of a cDNA library. As expected for a compact genome, sizes of the identified genes and introns are very small, and intergenic distances are short. Among identified genes, three code for amylases. As in mammals, these genes are linked, but they are found in a small region of less than 11 kb. These results represent the first description of a genomic sequence larger than 100 kb in this species. Synteny with the human genome is restricted to three regions corresponding to human 1p32.3, 1p13.3 and 1p21.1.
Genoscope/Centre National de Séquençage and CNRS-UMR 8030, 2 rue Gaston Crémieux, F-91057 Evry, France.
Laurence
L
Bouneau
Guillaume
G
Lardier
Cécile
C
Fischer
Muriel
M
Ronsin
Jean
J
Weissenbach
Alain
A
Bernot
D000595:Amino Acid Sequence; D000681:Amylases / Q000235:genetics*; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012984:Software; D026801:Synteny; D023721:Tetraodontiformes / Q000235:genetics*
D018076:DNA, Complementary; D004247:DNA; D000681:Amylases