Patterns of gene expressions induced by arsenic trioxide in cultured human fibroblasts.
Arsenic exposure is associated with several human diseases and particularly, with neoplasia. Although the mechanism of arsenic toxicity is not fully understood, several recent works pointed out the involvement of oxidative stress in arsenic-induced DNA damage that, in living cells, correlates with changes in gene expressions. In cultured human fibroblasts exposed for 24 h to micromolar arsenic concentrations, we studied, using real-time RT-PCR, the expression profile of a limited number of genes: genes coding for a stress protein (HSP70), transcription factors (cJUN, cFOS, ETR103, ETR101 and TTP) and cell cycle or DNA repair proteins (P21, GADD153). We observed that the expression profile of genes followed individual different patterns that can be summed up in early-transient gene expression by contrast to delayed gene expression.
Laboratoire de Biogénotoxicologie et Mutagenèse Environnementale (EA 1784-IFR PMSE 112), Faculté de Médecine, Université de la Méditerranée, 27 boulevard Jean Moulin, 13385 cedex 05, Marseille, France.
Vanina
V
Burnichon
Séverine
S
Jean
Laurence
L
Bellon
Marie
M
Maraninchi
Chantal
C
Bideau
Thierry
T
Orsière
Alain
A
Margotat
Victoria
V
Gérolami
Alain
A
Botta
Jean Louis
JL
Bergé-Lefranc
D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D010087:Oxides / Q000494:pharmacology*; D013997:Time Factors
D001152:Arsenicals; D010087:Oxides; D000077237:Arsenic Trioxide