[Effect on proliferation and apoptosis of human lingual carcinoma cells cotransfected by bax and p53 genes].
OBJECTIVE
To investigate the effects on proliferation and apoptosis of human cancerous cells cotransfected by bax apoptosis-inducing gene and p53 tumor suppressor gene.


METHODS
The chimeric gene pSV-CIP-bax-CAT was constructed in which bax gene was flanked upstream by a 217 bp fragment(+822(-)+1093) of the first intron and a 317 bp promoter fragment of human a 1(I) collagen gene. Human lingual carcinoma cell line Tca 8113 (LCC) in culture was respectively transfected with pSV-CIP-bax-CAT, and cotransfected with pSV-p53-CAT and pSV-CIP-bax-CAT by using the transfection reagent DOSPER.


RESULTS
Immuno-slot blot and ELISA demonstrated that the expression of bax and p53 genes increased remarkably in the transfected and cotransfected LCC, compared with the controls (LCC transfected with pSV-CIP-CAT and LCC). MTT colorimetric assay, TUNEL fluorenscence microscopy and flow cytometry showed that foreign bax or p53 gene inhibited the LCC growth and induced apoptosis (23.9% and 26.1% of inhibitory rate by bax gene and p53 plus bax genes respectively).


CONCLUSIONS
The ectopic expression of the bax gene in the LCC promoted by the cis-acting elements of human a1 (I)collagen gene has obviously synergistic effect on the proliferation and apoptosis of the LCC contransfected with p53 gene plus bax gene for 48 hours.
Ma
Y
Y
Institute of Biochemistry and Molecular Biology, School of Basic Medical Sciences, WCUMS, Chengdu 610041, China.
Chen
J
J
Gao
J
J
Peng
W
W
Wang
R
R
Yang
S
S
D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D002455:Cell Division; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D019253:Proto-Oncogene Proteins c-bcl-2; D014062:Tongue Neoplasms / Q000235:genetics* / Q000473:pathology; D014162:Transfection; D014407:Tumor Cells, Cultured; D051028:bcl-2-Associated X Protein
C494826:BAX protein, human; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein