Comparison of the standard AGID test and competitive ELISA for detecting bluetongue virus antibodies in camels in Gujarat, India.
The performance of the standard agar gel immunodiffusion (AGID) test and the competitive enzyme-linked immunosorbent assay (cELISA) for the detection of serum antibody against bluetongue virus (BTV) in clinically healthy and diseased camels in Gujarat state were compared. Out of 176 sera tested, 22 (12.5%) and 34 (19.3%) were positive for group-specific bluetongue antibodies by AGID and cELISA, respectively. Maximum seropositivities of 18.0% by AGID and 25.8% by cELISA were recorded in the Kutchhi breed, and of 6.9% and 12.6%, respectively, in the Marwari breed. The seroprevalence detected by AGID and cELISA in clinically healthy and diseased camels did not differ significantly with regard to bluetongue disease in these breeds.
Chandel
B S
BS
Department of Microbiology, College of Veterinary Science and Animal Husbandry, Gujarat Agricultural University, Sardar Krushinagar, India.
Chauhan
H C
HC
Kher
H N
HN
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D001819:Bluetongue / Q000175:diagnosis* / Q000276:immunology; D001820:Bluetongue virus / Q000276:immunology*; D002162:Camelus / Q000276:immunology / Q000821:virology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005779:Immunodiffusion / Q000662:veterinary*; D007194:India; D013045:Species Specificity
D000914:Antibodies, Viral