The automation of a commercial Fourier transform mass spectrometer to provide a quick and robust method for determining exact mass for the synthetic chemist.
Automation of a commercially available Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometer for the routine analysis of the synthetic products from high-speed chemistry is described. The automation includes software written by the instrument manufacturer and in-house developed software; allowing electronic submission of samples from the chemist and e-mailing of results back to the chemist. The use of samples of relatively high concentration (ca 1 mg x mL(-1)) is possible due to the protocol that has been developed, which includes dilution by the autosampler during sample injection. Though high concentrations are used for speed and convenience the amount of sample consumed is still small ca 15 microg per injection. The results from this method have been shown to be both accurate (average error +/- 0.91 ppm) and precise (-0.70 ppm to 2.26 ppm). The system is capable of analysing up to 800 samples per 24 hours. As high speed chemistry becomes more highly utilised within discovery the number of samples requiring accurate mass analysis will rise, and the method we have described will prevent high resolution mass spectrometry becoming the bottleneck in new chemical entity production. The accuracy and precision demonstrated by this method allows high confidence levels in assigned molecular formulae for expected compounds and reduces the number of possible formulae to consider when working with a compound that is not the desired product of a given reaction.
Pfizer Global Research and Development, Ramsgate Road, Sandwich, Kent, CT13 9NJ, UK.
Sarah
S
Dykes
Sally-Ann
SA
Fancy
George L
GL
Perkins
Frank S
FS
Pullen
D001331:Automation; D005583:Fourier Analysis; D007660:Ketoprofen / Q000032:analysis / Q000737:chemistry; D013058:Mass Spectrometry / Q000295:instrumentation* / Q000379:methods*; D008970:Molecular Weight; D012110:Reserpine / Q000032:analysis / Q000737:chemistry; D012680:Sensitivity and Specificity
D012110:Reserpine; D007660:Ketoprofen