[Ototoxic study spiral ganglion neurons in the pig guinea with ethylene oxide remained in absorbable gelatin sponge].
OBJECTIVE
To measure ethylene oxide remained in absorbable gelatin sponge, and to observe change of ultrastructure in spiral ganglion neurons after deposited in otic vesicle of pig guinea with ethylene oxide sterilized absorbable gelatin sponge.


METHODS
To use comparative colour technique measuring ethylene oxide remained in absorbable gelatin sponge, by means of transmission electron microscope to observe change of ultrastructure in spiral ganglion neurons after 3 months deposited in otic vesicle of pig guinea with ethylene oxide sterilized absorbable gelatin sponge.


RESULTS
The mean value of ethylene oxide remained in absorbable gelatin sponge were 438.32 mg, while nation standard are less 10 mg, FDA standard(1978) less 25 mg. ultrastructural appearance showed condensed matric electron dense of spiral ganglion mitochondria and dark crista of mitochondria, vacuolated cytoplasm. Ultrastructural observation also demonstrated dissolution or necrosis of ganglion cells, satellite cells, and vacuolated myelin. These changes were near Donson reported(1997) intracochlear perfusion with aminoglycosides.


CONCLUSIONS
The mean value of ethylene oxide remained in absorbable gelatin sponge were 40 times nation standard. Toxic changes of ethylene oxide remained in absorbable gelatin sponge in spiral ganglion neurons were similar to intracochlear perfusion with aminoglycosides.
Affiliated Eye Ear Nose Throat Hospital, Shanghai Fudan University, Shanghai 200031.
Yan
Y
Shen
Yunzhen
Y
Shen
Zhengmin
Z
Wang
Nianzu
N
Chen
Donglei
D
Wang
D000818:Animals; D004202:Disinfectants / Q000032:analysis / Q000506:poisoning*; D005027:Ethylene Oxide / Q000032:analysis / Q000506:poisoning*; D005781:Gelatin Sponge, Absorbable / Q000737:chemistry*; D006168:Guinea Pigs; D013136:Spiral Ganglion / Q000187:drug effects* / Q000648:ultrastructure
D004202:Disinfectants; D005027:Ethylene Oxide