[Synthesis and identification of methylparathion artificial antigen].
In order to synthesize the artificial antigen methylparathion(M1605), methylparathion was reduced into amino-methylparathion by using acetic acid-zinc powder-hydrochloric acid. Artificial antigens (M1605-BSA, M1605-TTH) were synthesized by conjugating amino-methylparathion to bovine serum albumin(BSA) and tachypleus tridentatus hemocyanin (TTH) directly after diazotization. Rabbits were immunized with M1605-BSA for 10 weeks, and the high titer and high specificity antiserum from those rabbits was testified by double agar gel diffusion and indirect ELISA. The results showed that an artificial antigen was obtained successfully and this made it possible to establish the immunoassay of M1605.
Institute of Occupational Medicine, Chinese Academy of Preventive Medicine, Beijing 100050, China.
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D000818:Animals; D000941:Antigens / Q000276:immunology*; D005260:Female; D006433:Hemocyanins / Q000276:immunology; D007306:Insecticides / Q000276:immunology*; D008743:Methyl Parathion / Q000276:immunology*; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000276:immunology
D000941:Antigens; D007306:Insecticides; D012710:Serum Albumin, Bovine; D008743:Methyl Parathion; D006433:Hemocyanins