No change in apoptosis in skeletal muscle exposed acutely or chronically to alcohol.
The pathogenic mechanisms responsible for the deleterious changes in ethanol-exposed skeletal muscle are unknown, although apoptosis may be a causal process. We therefore investigated the responses of skeletal muscle to acute or chronic ethanol exposure in male Wistar rats. In acute studies, rats were dosed with ethanol (75 mmol (3.46 g)/kg BW) and killed after either 2.5 or 6 hours. In chronic studies, rats were fed ethanol as 35% of total dietary energy for 6 weeks. Apoptosis was determined by either DNA fragmentation or TUNEL (terminal deoxynucleotidyl transferase mediated dUTP nick end labelling) assays. The results showed that apoptosis was not increased in the ethanol-exposed muscle in both acute and chronic studies compared to appropriate controls.
Paice
A G
AG
Department of Clinical Biochemistry, King's College School of Medicinem London, UK.
Hesketh
J E
JE
Towner
P
P
Hirako
M
M
Peters
T J
TJ
Preedy
V R
VR
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053938:DNA Fragmentation / Q000187:drug effects; D000431:Ethanol / Q000633:toxicity*; D020287:In Situ Nick-End Labeling; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
D000431:Ethanol