Cloning, sequencing and recombinant expression of the open reading frame encoding a novel member of the Sarcocystis muris (Apicomplexa) microneme lectin family.
Micronemes are characteristic secretory organelles located within the apical cell region of apicomplexan parasites. The protein contents are exocytosed during an early phase of host cell invasion and contribute to parasite motility and the invasion of target cells. We report here on the cloning and heterologous expression of a novel member of the Sarcocystis muris microneme lectin family. The deduced amino acid sequence is in total agreement with that obtained after sequencing the native protein and is characterized by two copies of the apple domain motif. The recombinant polypeptide is expressed in a biologically active conformation as demonstrated by its galactose binding properties.
Paul-Ehrlich-Institute, Division Parasitology/Diagnostics, Paul-Ehrlich-Strasse 51-59, 63225 Langen, Germany.
Harald
H
Klein
Stefanie
S
Mueller
Bettina
B
Loeschner
Ralf R
RR
Toenjes
Gundula
G
Braun
Eva-Christina
EC
Mueller
Albrecht
A
Otto
Thomas
T
Montag
D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000302:isolation & purification; D016367:Baculoviridae / Q000235:genetics; D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D037161:Galectins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D005786:Gene Expression Regulation; D017125:Genes, Protozoan; D007313:Insecta; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis; D012522:Sarcocystis / Q000737:chemistry / Q000166:cytology / Q000235:genetics*; D016415:Sequence Alignment
D000953:Antigens, Protozoan; D037161:Galectins; D011994:Recombinant Proteins