Nitric oxide and HSV vaginal infection in BALB/c mice.
Here we study the role of nitric oxide in the vaginal infection of Balb/c mice with herpes simplex virus type 2. Inducible nitric oxide synthase (iNOS) mRNA was detected by RT-PCR in vaginal tissue and inguinal lymph nodes early postinfection. iNOS was also found to be activated in cells recovered from vaginal washings of infected animals. Animals treated with aminoguanidine (AG), an iNOS inhibitor, showed a dose-dependent increase in vaginal pathology after viral infection compared to controls. Viral titers in vaginal washings and vaginas were higher in AG-treated mice. Treated animals presented higher PMN counts in vaginal washings compared to controls. Histopathology studies revealed a profound inflammatory exudate in vaginal tissue of treated animals. Finally, RT-PCR analysis showed increased expression of the chemokines MIP-2 and RANTES in vaginal tissue and inguinal lymph nodes of these animals.
Laboratory of Immunochemistry, Department of Biological Chemistry, Faculty of Sciences, University of Buenos Aires, Pabellón II, Piso 4, Ciudad Universitaria, (1428) Buenos Aires, Argentina. fbenencia@hotmail.com
Fabián
F
Benencia
Gisela
G
Gamba
Hernan
H
Cavalieri
Maria Cecilia
MC
Courreges
Ruben
R
Benedetti
Soledad Maria
SM
Villamil
Ernesto Jorge
EJ
Massouh
D000818:Animals; D001483:Base Sequence; D002455:Cell Division / Q000187:drug effects; D018946:Chemokine CCL5 / Q000235:genetics; D054426:Chemokine CXCL2; D018925:Chemokines / Q000235:genetics; D002522:Chlorocebus aethiops; D017931:DNA Primers; D005260:Female; D006558:Herpes Genitalis / Q000276:immunology / Q000503:physiopathology*; D018258:Herpesvirus 2, Human / Q000472:pathogenicity* / Q000502:physiology; D008198:Lymph Nodes / Q000201:enzymology / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000502:physiology*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000235:genetics*; D052247:Nitric Oxide Synthase Type II; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D026423:S-Nitroso-N-Acetylpenicillamine / Q000494:pharmacology; D014621:Vagina / Q000201:enzymology / Q000821:virology; D014709:Vero Cells; D014779:Virus Replication
D018946:Chemokine CCL5; D054426:Chemokine CXCL2; D018925:Chemokines; C404915:Cxcl2 protein, mouse; D017931:DNA Primers; D020030:Nitric Oxide Donors; D012333:RNA, Messenger; D009569:Nitric Oxide; D026423:S-Nitroso-N-Acetylpenicillamine; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse