Global stage-specific gene regulation during the developmental cycle of Chlamydia trachomatis.
Distinct morphological changes associated with the complex development cycle of the obligate intracellular bacterial pathogen Chlamydia trachomatis have been historically well characterized by microscopy. A number of temporally regulated genes have been characterized previously, suggesting that the chlamydial developmental cycle is regulated at the transcriptional level. This hypothesis was tested by microarray analysis in which the entire C. trachomatis genome was analyzed, providing a comprehensive assessment of global gene regulation throughout the chlamydial developmental cycle. Seven temporally cohesive gene clusters were identified, with 22% (189 genes) of the genome differentially expressed during the developmental cycle. The correlation of these gene clusters with hallmark morphological events of the chlamydial developmental cycle suggests three global stage-specific networks of gene regulation.
Division of Infectious Diseases, School of Public Health, University of California, Berkeley, California 94720, USA.
Tracy L
TL
Nicholson
Lynn
L
Olinger
Kimberley
K
Chong
Gary
G
Schoolnik
Richard S
RS
Stephens
D002692:Chlamydia trachomatis / Q000235:genetics* / Q000254:growth & development*; D015964:Gene Expression Regulation, Bacterial; D005810:Multigene Family; D020411:Oligonucleotide Array Sequence Analysis; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction