A novel cytomedical vehicle capable of protecting cells against complement.
We have developed "Cytomedicine," which consists of functional cells entrapped in semipermeable polymer, and previously reported that APA microcapsules could protect the entrapped cells from injury by cellular immune system. However, microencapsulated cells were not protected from humoral immune system. Here, we developed a novel APA microcapsule, in which APA microbeads (APA(Ba) microbeads) were modified to contain a barium alginate hydrogel within their centers in an attempt to make it more difficult for antibody and complement to permeate the microcapsules. The permeability of APA(Ba) microbeads was clearly less than that of APA microcapsules, presumably due to the presence of barium alginate hydrogel. Cells encapsulated within APA(Ba) microbeads were protected against treatment with xenogeneic anti-serum. Furthermore, murine pancreatic beta-cells encapsulated in APA(Ba) microbeads remained viable and continued to secrete insulin in response to glucose. Therefore, APA(Ba) microbeads may be a useful carrier for developing anti-complement device for cytomedical therapy.
Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka 565-0871, Japan.
Yasuo
Y
Yoshioka
Ryo
R
Suzuki
Hiroaki
H
Oka
Naoki
N
Okada
Takayuki
T
Okamoto
Tatsunobu
T
Yoshioka
Yohei
Y
Mukai
Hiroko
H
Shibata
Yasuo
Y
Tsutsumi
Shinsaku
S
Nakagawa
Jun-ichi
J
Miyazaki
Tadanori
T
Mayumi
D000464:Alginates / Q000737:chemistry*; D000818:Animals; D000910:Antibodies, Heterophile / Q000633:toxicity; D001464:Barium / Q000737:chemistry; D003169:Complement Inactivator Proteins / Q000737:chemistry; D003165:Complement System Proteins / Q000378:metabolism / Q000633:toxicity*; D019610:Cytoprotection; D020100:Hydrogels / Q000737:chemistry; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000378:metabolism; D008567:Membranes, Artificial; D051379:Mice; D008863:Microspheres; D010539:Permeability; D011107:Polylysine / Q000031:analogs & derivatives* / Q000737:chemistry*; D014407:Tumor Cells, Cultured
D000464:Alginates; D000910:Antibodies, Heterophile; D003169:Complement Inactivator Proteins; D020100:Hydrogels; D007328:Insulin; D008567:Membranes, Artificial; C051546:alginate-polylysine-alginate; D001464:Barium; D011107:Polylysine; D003165:Complement System Proteins