Induction of broad and potent anti-human immunodeficiency virus immune responses in rhesus macaques by priming with a DNA vaccine and boosting with protein-adsorbed polylactide coglycolide microparticles.
Several vaccine technologies were evaluated for their abilities to induce anti-human immunodeficiency virus Gag immune responses in rhesus macaques. While no vaccine alone was able to induce broad and strong immune responses, these were achieved by priming with Gag DNA and boosting with Gag protein adsorbed to polylactide coglycolide microparticles. This regimen elicited strong antibodies, helper T cells, and cytotoxic T lymphocytes and thus holds promise as an effective vaccination scheme.
Otten
Gillis
G
Vaccines Research, Chiron Corporation, Emeryville, California 94608, USA. Gillis_Otten@chiron.com
Schaefer
Mary
M
Greer
Catherine
C
Calderon-Cacia
Maria
M
Coit
Doris
D
Kazzaz
Jina
J
Medina-Selby
Angelica
A
Selby
Mark
M
Singh
Manmohan
M
Ugozzoli
Mildred
M
zur Megede
Jan
J
Barnett
Susan W
SW
O'Hagan
Derek
D
Donnelly
John
J
Ulmer
Jeffrey
J
D016915:AIDS Vaccines / Q000008:administration & dosage / Q000276:immunology*; D000818:Animals; D015683:Gene Products, gag / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D015483:HIV Antibodies / Q000097:blood; D015658:HIV Infections / Q000276:immunology / Q000517:prevention & control; D006801:Humans; D007114:Immunization; D007117:Immunization, Secondary; D019344:Lactic Acid / Q000276:immunology*; D008213:Lymphocyte Activation; D008253:Macaca mulatta; D008863:Microspheres; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D013601:T-Lymphocytes / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*
D016915:AIDS Vaccines; D015683:Gene Products, gag; D015483:HIV Antibodies; D011108:Polymers; D019444:Vaccines, DNA; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid