Ryegrass-based diet and barley supplementation: partition of energy-yielding nutrients among splanchnic tissues and hind limbs in finishing lambs.
Splanchnic metabolism of energy-yielding nutrients and their uptake by the hind limb were studied in finishing lambs receiving ryegrass harvested at grazing stage (ear at 10 cm) with or without barley supplementation. Six ruminally cannulated and multicatherized lambs (40.2 +/- 1.5 kg) were fed with frozen ryegrass (RG) at 690 kJ of metabolizable energy intake (MEI) x d(-1) x BW(-0.75) successively with and without barley supplementation (RG + B), according to a triplicated Latin square design. Barley supplementation represented 21% of DM intake and increased the MEI by 32% (P < 0.002). In ruminal fluid, barley supplementation increased the acetate and butyrate concentrations by 21.2 and 49.6%, respectively (P < 0.04), without modifying those of propionate. Thus, molar proportions of acetate and butyrate were not modified, and those of propionate tended (P < 0.06) to decrease from 26 to 23%. As a result, the net portal appearance of propionate was not modified. Net portal appearance of butyrate and beta-hydroxybutyrate increased (P < 0.03), and that of acetate was not modified. Consequently, hepatic uptake of butyrate increased and probably spared acetate from hepatic metabolism. The hepatic fractional extraction of propionate decreased (P < 0.03), whereas the net flux of lactate switched from a net release to a net uptake, suggesting an alteration in the contribution of gluconeogenic substrates to glucose synthesis without modification in net hepatic glucose release. As a consequence, barley supplementation increased net splanchnic release of acetate (P < 0.02), propionate (P < 0.001), and beta-hydroxybutyrate (P < 0.01) by 60, 157, and 78%, respectively. In addition, the net splanchnic release of insulin increased (P < 0.03) because of a decrease (P < 0.02) in its hepatic extraction. Despite those changes, the net uptake of nutrients by the hind limb was not modified and even decreased in the case of glucose (P < 0.02), suggesting a stimulation of lipogenesis in adipose tissues. Results from the present study suggested that supplementation of a ryegrass-based diet would likely have little effect on the orientation of muscle energy metabolism and on meat quality because the net uptake of nutrients by the hind limb was unchanged.
Majdoub
L
L
Unité de Recherches sur les Herbivores, INRA, Theix, 63122 Saint Genès Champanelle, France.
Vermorel
M
M
Ortigues-Marty
I
I
D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D019587:Dietary Supplements; D004734:Energy Metabolism / Q000502:physiology*; D005232:Fatty Acids, Volatile / Q000097:blood*; D005943:Gluconeogenesis; D006614:Hindlimb / Q000378:metabolism*; D001467:Hordeum / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008129:Lolium / Q000378:metabolism; D008297:Male; D008460:Meat / Q000592:standards; D011169:Portal Vein; D012756:Sheep / Q000378:metabolism*; D013152:Splanchnic Circulation; D014781:Viscera / Q000378:metabolism*
D005232:Fatty Acids, Volatile; D007328:Insulin