Guidelines for using in vitro methods to study the effects of phyto-oestrogens on bone.
These guidelines review the relevant literature on the way plant phyto-oestrogens act on bone and the responsiveness of different bone cell systems to phyto-oestrogenic compounds. The primary emphasis is on the experimental conditions used, the markers available for assessing osteoblast and osteoclast function, and their expected sensitivity. Finally, we assess the published results to derive some general recommendations for in vitro experiments in this area of research.
Lieberherr
Michèle
M
Laboratoire de Nutrition et de Sécurité Alimentaire, Institut National de la Recherche Agronomique, F-78350 Jouy-en-Josas, France. lieberhe@jouy.inra.fr
Cournot
Giulia
G
Robins
Simon P
SP
D001842:Bone and Bones / Q000187:drug effects*; D018929:Cell Culture Techniques / Q000379:methods; D004968:Estrogens, Non-Steroidal / Q000494:pharmacology*; D023281:Genomics; D017408:Guidelines as Topic; D006801:Humans; D007529:Isoflavones; D009924:Organ Culture Techniques / Q000379:methods; D010006:Osteoblasts / Q000187:drug effects; D010010:Osteoclasts / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects; D048789:Phytoestrogens; D028321:Plant Preparations
D004968:Estrogens, Non-Steroidal; D007529:Isoflavones; D048789:Phytoestrogens; D028321:Plant Preparations