Presence of free D-amino acids in microalgae.
Several species of microalgae (phytoplankton), 4 species of freshwater algae and 4 species of marine diatoms, were cultured germ-free in the laboratory. The presence of free D-amino acids was verified in these species by a reversed-phase HPLC analysis. D-Aspartate was detected in all the microalgae examined, but D-alanine was only present in the marine diatoms. The D-amino acid content in Asterionella sp. of the marine diatoms increased from the exponential phase to the stationary phase and then decreased to the phase of decline.
Yokoyama
Takehiko
T
School of Fisheries Sciences, Kitasato University, Ofunato, Iwate 022-0101, Japan.
Kan-no
Nobuhiro
N
Ogata
Takehiko
T
Kotaki
Yuichi
Y
Sato
Minoru
M
Nagahisa
Eizoh
E
D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D056890:Eukaryota / Q000737:chemistry*; D005618:Fresh Water; D010839:Phytoplankton / Q000737:chemistry*; D012623:Seawater
D000596:Amino Acids