Up-regulation of soyasaponin biosynthesis by methyl jasmonate in cultured cells of Glycyrrhiza glabra.
Exogenously applied methyl jasmonate (MeJA) stimulated soyasaponin biosynthesis in cultured cells of Glycyrrhiza glabra (common licorice). mRNA level and enzyme activity of beta-amyrin synthase (bAS), an oxidosqualene cyclase (OSC) situated at the branching point for oleanane-type triterpene saponin biosynthesis, were up-regulated by MeJA, whereas those of cycloartenol synthase, an OSC involved in sterol biosynthesis, were relatively constant. Two mRNAs of squalene synthase (SQS), an enzyme common to both triterpene and sterol biosyntheses, were also up-regulated by MeJA. In addition, enzyme activity of UDP-glucuronic acid: soyasapogenol B glucuronosyltransferase, an enzyme situated at a later step of soyasaponin biosynthesis, was also up-regulated by MeJA. Accumulations of bAS and two SQS mRNAs were not transient but lasted for 7 d after exposure to MeJA, resulting in the high-level accumulation (more than 2% of dry weight cells) of soyasaponins in cultured licorice cells. In contrast, bAS and SQS mRNAs were coordinately down-regulated by yeast extract, and mRNA accumulation of polyketide reductase, an enzyme involved in 5-deoxyflavonoid biosynthesis in cultured licorice cells, was induced transiently by yeast extract and MeJA, respectively.
Hayashi
Hiroaki
H
Department of Pharmacognosy, Gifu Pharmaceutical University, Mitahora-higashi 5-6-1, Gifu, 502-8585 Japan. hayashih@gifu-pu.ac.jp
Huang
Pengyu
P
Inoue
Kenichiro
K
D000085:Acetates / Q000494:pharmacology*; D002478:Cells, Cultured; D003517:Cyclopentanes / Q000494:pharmacology*; D013186:Farnesyl-Diphosphate Farnesyltransferase / Q000235:genetics / Q000378:metabolism; D014453:Glucuronosyltransferase / Q000187:drug effects / Q000378:metabolism; D006035:Glycyrrhiza / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D019751:Intramolecular Transferases / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D054883:Oxylipins; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012503:Saponins / Q000096:biosynthesis* / Q000378:metabolism
D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D012333:RNA, Messenger; D012503:Saponins; C407096:soyasapogenol B; C056684:soyasaponin I; C106126:soyasaponin II; D009828:Oleanolic Acid; C072239:methyl jasmonate; D014453:Glucuronosyltransferase; D013186:Farnesyl-Diphosphate Farnesyltransferase; D019751:Intramolecular Transferases; C039924:2,3-oxidosqualene-beta-amyrin-cyclase; C021924:lanosterol synthase