The development of clinical activity in relapsing-remitting MS is associated with a decrease of FasL mRNA and an increase of Fas mRNA in peripheral blood.
In this longitudinal study, we examined the expression of Fas, FasL, CCR3, CCR5 and CXCR3 mRNA in peripheral blood mononuclear cells (PBMCs) of secondary progressive (SP) and relapsing-remitting (RR) multiple sclerosis (MS) patients. In RR patients, FasL, CCR3 and CCR5 mRNA levels were increased prior to the exacerbations, but these decreased during clinical activity, while mRNA levels of Fas increased. SP patients have increased the levels of Fas and FasL mRNA; the latter was particularly increased during lesional activity. Our data support the hypothesis that changes in Fas and FasL mRNA related to clinical activity are due to the migration of inflammatory cells to the central nervous system (CNS).
Division of Immunological and Infectious Diseases, TNO Prevention and Health, P.O. Box 2215, 2301 CE, Leiden, The Netherlands.
Luba
L
Lopatinskaya
Anette H H
AH
van Boxel-Dezaire
Frederik
F
Barkhof
Chris H
CH
Polman
Cornelis J
CJ
Lucas
Lex
L
Nagelkerken
D000328:Adult; D017209:Apoptosis / Q000276:immunology; D065100:CCR5 Receptor Antagonists; D003430:Cross-Sectional Studies; D015536:Down-Regulation / Q000235:genetics / Q000276:immunology*; D053222:Fas Ligand Protein; D006801:Humans; D008024:Ligands; D008137:Longitudinal Studies; D008562:Membrane Glycoproteins / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000235:genetics; D008875:Middle Aged; D020528:Multiple Sclerosis, Chronic Progressive / Q000097:blood / Q000276:immunology / Q000473:pathology; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000097:blood / Q000276:immunology* / Q000473:pathology; D012333:RNA, Messenger / Q000037:antagonists & inhibitors* / Q000096:biosynthesis*; D054397:Receptors, CCR3; D019713:Receptors, CCR5 / Q000235:genetics; D054367:Receptors, CXCR3; D019707:Receptors, Chemokine / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics / Q000276:immunology*; D019014:fas Receptor / Q000096:biosynthesis* / Q000097:blood* / Q000235:genetics
C515350:CCR3 protein, human; D065100:CCR5 Receptor Antagonists; C515187:CXCR3 protein, human; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D008024:Ligands; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D054397:Receptors, CCR3; D019713:Receptors, CCR5; D054367:Receptors, CXCR3; D019707:Receptors, Chemokine; D019014:fas Receptor