[Mutation of p53 and Ki-ras gene in human fetal lung fibroblast cells in vitro by sterigmatocystin].
To explore the carcinogenic effects of sterigmatocystin(ST), one of the predominant contaminating mycotoxins in high risk areas of cancer in China, mutation of tumor suppressor gene p53 and oncogene Ki-ras in human fetal lung cells in vitro induced by ST was studied using cell culture and silver-staining PCR-SSCP methods. The results showed that, within 22 weeks of ST treatment, no abnormalities were found for both p53 and Ki-ras gene in electrophoresis. Abnormal electrophoretic migration bands were seen at exon 8 of p53 gene and Ki-ras gene in ST-treated human lung fibroblast cells 22 weeks after ST treatment. Thus, the results further confirmed the carcinogenic effects of ST on human fetal lung tissue.
Cao
W
W
Institute of Basic Medical Science, Hebei Medical University, Shijiazhuang 050017, China.
Wang
H
H
Zhang
X
X
Sun
X
X
D002273:Carcinogens / Q000633:toxicity; D005333:Fetus; D005347:Fibroblasts / Q000166:cytology; D016158:Genes, p53; D011905:Genes, ras; D006801:Humans; D008168:Lung / Q000166:cytology* / Q000378:metabolism; D009154:Mutation; D013241:Sterigmatocystin / Q000633:toxicity*
D002273:Carcinogens; D013241:Sterigmatocystin