Helicobacter pylori lipopolysaccharide in the IL-2 milieu activates lymphocytes from dyspeptic children.
In this study, we assessed the proliferative response of peripheral blood mononuclear leukocytes (PBML) from 33 children/young adolescents with chronic dyspepsia, to H. pylori LPS in the presence and absence of IL-2 as a T cell growth factor. A rapid urease test (RUT) and a presence of Helicobacter-like organisms (HLO) in the biopsy specimens allowed us to distinguish RUT/HLO-positive (17/33) and -negative (16/33) patients. H. pylori LPS alone induced a proliferation of PBML from 4 out of 33 dyspeptic patients. IL-2 increased the prevalence of the response to LPS to 59% and 74% of RUT/HLO-positive and -negative patients, respectively. PBML from RUT/HLO-positive patients responded significantly less intensively to H. pylori LPS in the presence of IL-2, to IL-2 alone and to H. pylori LPS+IL-2. However, there was no difference in PHA-driven proliferation of PBML from the patients of those two groups. A negative correlation between the responsiveness to H. pylori LPS of PBML and occurrence of type B inflammation in gastric mucosa was demonstrated. The results suggest a contribution of H. pylori LPS to an outcome of H. pylori infection. It is speculated that H. pylori LPS by an activation of immunocompetent cells may reduce gastric inflammation, decrease bacterial load and prolong H. pylori infection.
Department of Immunology and Infectious Biology, Institute for Microbiology and Immunology, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland. rudw@biol.uni.lodz.pl
Wieslawa
W
Rudnicka
Agnieszka
A
Jarosinska
Leokadia
L
Bak-Romaniszyn
Anthony
A
Moran
Izabela
I
Planeta-Malecka
Torkel
T
Wadstrom
Magdalena
M
Chmiela
D000293:Adolescent; D002478:Cells, Cultured; D002648:Child; D002908:Chronic Disease; D004415:Dyspepsia / Q000276:immunology*; D016481:Helicobacter Infections / Q000276:immunology / Q000382:microbiology; D016480:Helicobacter pylori / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D007249:Inflammation; D007376:Interleukin-2 / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology*; D008213:Lymphocyte Activation; D014510:Urease / Q000378:metabolism
D007376:Interleukin-2; D008070:Lipopolysaccharides; D014510:Urease