Expression of novel rice gibberellin 2-oxidase gene is under homeostatic regulation by biologically active gibberellins.
We have cloned two genes for gibberellin (GA) 2-oxidase from rice ( Oryza sativa L.). Expression of OsGA2ox2 was not observed. The other gene, OsGA2ox3, was expressed in every tissue examined and was enhanced by the application of biologically active GA. Recombinant OsGA2ox3 protein catalyzed the metabolism of GA(1) to GA(8) and GA(20) to GA(29)-catabolite. These results indicate that OsGA2ox3 is involved in the homeostatic regulation of the endogenous level of biologically active GA in rice.
Sakai
Miho
M
National Institute of Agrobiological Sciences, Tsukuba, Japan.
Sakamoto
Tomoaki
T
Saito
Tamio
T
Matsuoka
Makoto
M
Tanaka
Hiroshi
H
Kobayashi
Masatomo
M
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D005875:Gibberellins / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000235:genetics*; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment
D005875:Gibberellins; D011994:Recombinant Proteins; D006899:Mixed Function Oxygenases; C121463:gibberellin 2-dioxygenase