Xylanase production by Trichoderma reesei rut C-30 on rice straw.
Xylanase production of Trichoderma reesei Rut C-30 was examined at different initial pH values (4.8, 5.9, and 7.0) on rice straw in shake flasks, and in a fermentor, for the best pH condition. Enzyme performance was tested on ammonia-treated dwarf elephant grass. The maximum xylanase activities, 92 and 122 IU/mL, were obtained at pH 4.8 in the shake flasks and fermentor, respectively, in which good growth of the fungus was observed during the first 24 h and consumption of proteins dissolved from the rice straw caused the pH to rise later to values between 6.4 and 6.7 (optimal for xylanase production). The xylanases from T. reesei were as effective as Multifect XL, a commercial enzyme preparation, in hydrolyzing ammonia-treated elephant grass.
Laboratorio de Alimentos, Departamento de Quimica, Facultad de Ciencias, Universidad del Zulia, Av. Universidad, Grano de Oro. Módulo 2, Maracaibo, Venezuela.
Alejandro
A
Colina
Betzabé
B
Sulbarán-De-Ferrer
Cateryna
C
Aiello
Alexis
A
Ferrer
D002480:Cellulase / Q000378:metabolism; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D012275:Oryza / Q000382:microbiology*; D014242:Trichoderma / Q000201:enzymology* / Q000254:growth & development; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000096:biosynthesis*
D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase; D002480:Cellulase