Structural analysis of the lipooligosaccharide from the commensal Haemophilus somnus strain 1P.
The structure of the lipooligosaccharide (LOS) from the commensal Haemophilus somnus strain 1P was elucidated. The structure of the O-deacylated LOS was established by monosaccharide analysis, NMR spectroscopy and mass spectrometry. The following structure for the O-deacylated LOS was determined on the basis of the combined data from these experiments. [chemical structure: see text] In the structure Kdo is 3-deoxy-D-manno-octulosonic acid, Hep is L-glycero-D-manno-heptose and lipid A-OH refers to O-deacylated Lipid A. The elucidation of this structure has increased our understanding of the relationship between the variability in LOS structure and the pathogenic potential of this organism. Specifically, the inability of this commensal strain to sialylate its LOS suggests that LOS sialylation could be a crucial virulence factor for H. somnus.
Institute for Biological Sciences, National Research Council, Room 3101, 100 Sussex Drive, ON, K1A 0R6, Ottawa, Canada. andrew.cox@nrc-cnrc.gc.ca
Andrew D
AD
Cox
Michael D
MD
Howard
Thomas J
TJ
Inzana
D006190:Haemophilus / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry
D008070:Lipopolysaccharides; C023023:lipid-linked oligosaccharides