HP1/ORC complex and heterochromatin assembly.
We have used the highly conserved heterochromatin component, heterochromatin protein 1 (HP1), as a molecular tag for purifying other protein components of Drosophila heterochromatin. A complex of HP1 associated with the origin recognition complex (ORC) and an HP1/ORC-associated protein (HOAP) was purified from the maternally loaded cytoplasm of early Drosophila embryo. We propose that the DNA-binding activities of ORC and HOAP function to recruit underphosphorylatedisoforms of HP1 to sites of heterochromatin nucleation. The roles of highly phosphorylated HP1, other DNA-binding proteins known to interact with HP1, and histone modifying activities in heterochromatin assembly are also addressed.
Shareef
Mohammed M
MM
School of Biological Sciences, 101 T.H. Morgan Building, University of Kentucky, Lexington, KY 40506, USA.
Badugu
RamaKrishna
R
Kellum
Rebecca
R
D000818:Animals; D000090266:Chromobox Protein Homolog 5; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics*; D002874:Chromosome Mapping; D004268:DNA-Binding Proteins / Q000235:genetics*; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics; D006570:Heterochromatin / Q000235:genetics*; D008957:Models, Genetic; D051738:Origin Recognition Complex
D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D006570:Heterochromatin; D051738:Origin Recognition Complex; C471206:cav protein, Drosophila; D000090266:Chromobox Protein Homolog 5