Determination of fleroxacin in pure and tablet forms by liquid chromatography and derivative UV-spectrophotometry.
Derivative UV-spectrophotometric and liquid chromatographic (LC) methods for fleroxacin determination were validated. In the spectrophotometric assay, first-, second-, third-, and fourth-order measurements were applied with the use of peak-zero and peak-peak techniques. The linear correlation between amplitude of the peak and concentration of the examined drug ranged from 2.0 to 12.0 micro/mL. An isocratic LC analysis was performed on a Purospher ODS column with an acidic mobile phase containing tetrabutylammonium hydroxide. Measurements were made at a wavelength of 285 nm with 4-aminobenzoic acid (PABA) as internal standard. The calibration curve was linear (r = 0.9999) in the studied range of concentration (1.0-10.0 microg/mL). The accuracy (mean recovery, about 100%), precision (relative standard deviation < 1%), selectivity, and sensitivity of the elaborated methods were satisfactory.
Kowalczuk
Dorota
D
Medical University, Department of Medicinal Chemistry, 6 Chodźki Str. 20-093 Lublin, Poland. kzchl@asklepios.am.lublin.pl
Hopkała
Hanna
H
D010129:4-Aminobenzoic Acid; D000890:Anti-Infective Agents / Q000032:analysis*; D002853:Chromatography, Liquid; D004340:Drug Contamination; D016576:Fleroxacin / Q000032:analysis*; D007202:Indicators and Reagents; D008670:Metals; D012015:Reference Standards; D015203:Reproducibility of Results; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D013607:Tablets
D000890:Anti-Infective Agents; D007202:Indicators and Reagents; D008670:Metals; D012996:Solutions; D013607:Tablets; D016576:Fleroxacin; D010129:4-Aminobenzoic Acid