A new sensitive cartridge-RIA method for determination of stavudine (D4T) triphosphate in human cells in vivo.
We describe a simple and sensitive method to determine stavudine triphosphate, the active intracellular anabolite of stavudine (D4T). Quantification of D4T triphosphate was performed with a combined cartridge-radioimmunoassay (cartridge-RIA) which enabled us to measure concentrations of D4T triphosphate as low as 0.5 ng/ml, or an intracellular concentration which corresponds to 20 fmol/10(6) cells if diluted like our previously published zidovudine (ZDV) assay. The only alternate methodology at present employs liquid chromatography mass spectroscopy (LC-MS/MS). The use of the cartridge-RIA methodology provides a cost-effective alternative for the determination of in vivo cellular pharmacokinetics studies of D4T in human immunodeficiency virus (HIV)-infected persons.
Department of Infectious Diseases, St Jude Children's Research Hospital, Memphis, TN 38105, USA.
Thu T
TT
Tran
Brian L
BL
Robbins
Frank H
FH
Pinkerton
Bernard
B
Ferrua
Jacques
J
Grassi
Arnold
A
Fridland
D006801:Humans; D007474:Ion Exchange; D007963:Leukocytes, Mononuclear / Q000737:chemistry / Q000378:metabolism; D011863:Radioimmunoassay / Q000379:methods*; D018894:Reverse Transcriptase Inhibitors / Q000032:analysis* / Q000097:blood; D012680:Sensitivity and Specificity; D018119:Stavudine / Q000032:analysis* / Q000097:blood
D018894:Reverse Transcriptase Inhibitors; D018119:Stavudine