Community-level physiological profiling performed with an oxygen-sensitive fluorophore in a microtiter plate.
Community-level physiological profiling based upon fluorometric detection of oxygen consumption was performed on hydroponic rhizosphere and salt marsh litter samples by using substrate levels as low as 50 ppm with incubation times between 5 and 24 h. The rate and extent of response were increased in samples acclimated to specific substrates and were reduced by limiting nitrogen availability in the wells.
Garland
Jay L
JL
Dynamac Corporation, Mail Code DYN-3, Kennedy Space Center, FL 32899, USA. jay.garland-1@ksc.nasa.gov
Roberts
Michael S
MS
Levine
Lanfang H
LH
Mills
Aaron L
AL
D015374:Biosensing Techniques; D017753:Ecosystem; D004783:Environmental Microbiology; D005456:Fluorescent Dyes; D018527:Hydroponics; D010100:Oxygen / Q000032:analysis*; D014908:Triticum / Q000254:growth & development / Q000382:microbiology
D005456:Fluorescent Dyes; D010100:Oxygen