In vitro dermal penetration study of carbofuran, carbosulfan, and furathiocarb.
In this study, the dermal penetration rate of carbofuran, carbosulfan, and furathiocarb has been measured with rat abdominal skin using the static diffusion cell. The technical grades of three compounds were applied at different doses on skin surface mounted in static diffusion cell and incubated at 32 degrees C for 48 h with shaking. The same procedures were carried out with furathiocarb EC (emulsifiable concentrate) and WP (wettable powder). At regular intervals, the receptor fluid in cell was sampled and analyzed by HPLC. Only carbofuran was found in carbosulfan- or furathiocarb-treated samples, suggesting they converted into carbofuran while passing through the skin layer. The quantity of insecticide penetrating skin increased with time and applied dose. The skin penetration rate increased with the water solubility of insecticides. The dermal penetration rates of carbofuran, furathiocarb, and carbosulfan were determined as 1.05 micro g/cm(2) per h ( r(2)=0.991), 0.46 micro g/cm(2) per h ( r(2)=0.984) and 0.14 micro g/cm(2) per h ( r(2)=0.967), respectively. There was no significant difference in rate of skin penetration between furathiocarb EC (1.42 micro g/cm(2) per h, r(2)=0.988) and WP (1.35 micro g/cm(2) per h, r(2)=0.982), while furathiocarb technical grade showed a lower skin penetration rate. In vitro models may be used to predict percutaneous absorption and are useful in selecting safer formulations for field application of pesticide.
Department of Pharmacology, Inje University, College of Medicine, 614-735, Busan, Korea.
Kwang-Hyeon
KH
Liu
Jeong-Han
JH
Kim
D000279:Administration, Cutaneous; D000818:Animals; D001572:Benzofurans / Q000493:pharmacokinetics*; D002219:Carbamates / Q000493:pharmacokinetics*; D002235:Carbofuran / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D007306:Insecticides / Q000493:pharmacokinetics*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000378:metabolism*; D012869:Skin Absorption / Q000187:drug effects* / Q000502:physiology; D012995:Solubility
D001572:Benzofurans; D002219:Carbamates; D007306:Insecticides; D002235:Carbofuran; C035038:carbosulfan; C052300:deltanit