The plasma membrane-associated NADH oxidase (ECTO-NOX) of mouse skin responds to blue light.
NADH oxidases of the external plasma membrane surface (ECTO-NOX proteins) are characterized by oscillations in activity with a regular period length of 24 min. Explants of mouse skin exhibit the oscillatory activity as estimated from the decrease in A(340) suggesting that individual ECTO-NOX molecules must somehow be induced to function synchronously. Transfer of explants of mouse skin from darkness to blue light (495 nm, 2 min, 50 micromol m(-1) s(-1)) resulted in initiation of a new activity maximum (entrainment) with a midpoint 36 min after light exposure followed by maxima every 24 min thereafter. Addition of melatonin resulted in a new maximum 24 min after melatonin addition. The findings suggest that the ECTO-NOX proteins play a central role in the entrainment of the biological clock both by light and by melatonin.
Department of Medicinal Chemistry and Molecular Pharmacology, Hansen Life Sciences Research Building, Purdue University, 201 S. University Street, West Lafayette, IN 47907-2064, USA. moore@pharmacy.purdue.edu
D James
DJ
Morré
Dorothy M
DM
Morré
D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D003624:Darkness; D007700:Kinetics; D008027:Light; D051379:Mice; D008810:Mice, Inbred C57BL; D009097:Multienzyme Complexes / Q000378:metabolism / Q000528:radiation effects*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism / Q000528:radiation effects*; D009924:Organ Culture Techniques; D012867:Skin / Q000201:enzymology* / Q000528:radiation effects
D009097:Multienzyme Complexes; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases