Humoral immune responses to S-layer-like proteins of Bacteroides forsythus.
Bacteroides forsythus is one of the important periodontopathic bacteria, and this microorganism is known to have an S-layer outside the outer membrane. The S-layer-like antigens were recently isolated from B. forsythus, and they were found to be 270- and 230-kDa proteins in the envelope fraction. In this study, these proteins were confirmed to be specific to B. forsythus by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and they were clearly recognized by sera from patients with adult and early-onset periodontitis in Western immmunoblot analysis. We compared the immunoglobulin G (IgG) responses against the purified S-layer-like antigen by enzyme-linked immunosorbent assay. IgG responses against this antigen were low in healthy control subjects, but they were significantly higher in subjects with adult and early-onset periodontitis. Together with the fact that the IgG responses against the crude extract of B. forsythus did not rise significantly in patients with periodontitis, S-layer-like proteins are considered to be specific antigens of B. forsythus and may play an important role in the progression of periodontitis.
Yoneda
Masahiro
M
Section of Periodontology, Department of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Japan. yoneda@dent.kyushu-u.ac.jp
Hirofuji
Takao
T
Motooka
Noriko
N
Nozoe
Koji
K
Shigenaga
Kayoko
K
Anan
Hisashi
H
Miura
Mayumi
M
Kabashima
Hiroaki
H
Matsumoto
Akiko
A
Maeda
Katsumasa
K
D000328:Adult; D017668:Age of Onset; D000917:Antibody Formation; D000942:Antigens, Bacterial / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000276:immunology* / Q000302:isolation & purification; D001439:Bacteroides / Q000737:chemistry / Q000276:immunology*; D015153:Blotting, Western; D016022:Case-Control Studies; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis; D008297:Male; D008565:Membrane Proteins / Q000276:immunology*; D008875:Middle Aged; D010518:Periodontitis / Q000276:immunology*
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007106:Immune Sera; D007074:Immunoglobulin G; D008565:Membrane Proteins