Genetics of cell-mediated lympholysis (CML) in man.
Human cytotoxic lymphocytes (CTL), developed in responder-stimulator combinations incompatible for only one HLA-B antigen, have been used to study the specificity and genetics of target cell determinants. We were able to develop specific CTLs directed against HLA-B7, B8, B27 and Bw44, as defined by standard serological reagents. CTL-anti-B5 and CTL-anti-Bw35 gave a number of false positive reactions. In addition, we have found that specific cytotoxic effector cells can be generated against B27 by sensitization of cells from a B7 donor and vice versa; B7 and B27 can in this way be recognized easily. The data support the view that HLA-B is the target antigen. In addition, population and family studies revealed that extra reactions with CTLs developed in female (parous) responder-male stimulator combinations were due to HLA-restricted H-Y antigen killing. These findings suggest that by selecting responder-stimulator pairs differing for only one antigen at a locus, it should be possible to develop CTLs which will allow recognition of other specificities at the HLA-B locus and other loci.
Department of Pathology, McMaster University, Hamilton, Ontario, Canada.
D P
DP
Singal
Y J
YJ
Wadia
D005190:Family; D006680:HLA Antigens / Q000235:genetics* / Q000276:immunology*; D015235:HLA-B Antigens / Q000235:genetics / Q000276:immunology; D015796:HLA-B27 Antigen / Q000235:genetics / Q000276:immunology; D059948:HLA-B44 Antigen; D015793:HLA-B7 Antigen / Q000235:genetics / Q000276:immunology; D015795:HLA-B8 Antigen / Q000235:genetics / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular / Q000235:genetics* / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D006680:HLA Antigens; D015235:HLA-B Antigens; D015796:HLA-B27 Antigen; D059948:HLA-B44 Antigen; D015793:HLA-B7 Antigen; D015795:HLA-B8 Antigen