Cross-reactive monoclonal antibodies raised against the lipopolysaccharide antigen of Salmonella minnesota Re chemotype: diagnostic relevance.
Three cross-reactive monoclonal antibodies (MAbs) of IgM and IgG2b isotype were generated in two separate fusions after immunization of BALB/c mice with heat killed Salmonella minnesota R595 of Re chemotype and acid-treated bacteria, coated with Re lipopolysaccharide (LPS) antigen. The specificity of the MAbs was demonstrated as the Re LPS antigen. The activity and cross-reactivity against purified elementary bodies of Chlamydia trachomatis and various S- and R-LPS antigens of other Gram-negative bacteria were characterized in enzyme-linked immunosorbent assay, passive hemolysis assay, immunoblot and immunofluorescence with the available chlamydial strains. The results demonstrated cross-reaction between the Re LPS antigen, the genus-specific chlamydial LPS and the LPS antigens of Escherichia coli O119 and Acinetobacter baumannii, suggesting the presence of identical or similar epitopes in the lipopolysaccharide antigens. The findings are implying the necessity of novel approaches, improving the specificity of serologic assays in the laboratory diagnosis of chlamydial infections.
Department of Microbiology, Medical University, Zdrave 2 str., 1431 Sofia, Bulgaria. mitov@medfac.acad.bg
Ivan
I
Mitov
Iana
I
Haralambieva
Dragomir
D
Petrov
Radka
R
Ivanova
Bojin
B
Kamarinchev
Ianko
I
Iankov
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002692:Chlamydia trachomatis / Q000276:immunology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D006090:Gram-Negative Bacteria / Q000276:immunology; D015151:Immunoblotting; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D012475:Salmonella / Q000145:classification* / Q000276:immunology*; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D008070:Lipopolysaccharides; C085483:Re lipopolysaccharide