Inhibitory effect of Adonis amurensis components on tube-like formation of human umbilical venous cells.
Antiangiogenic activity-guided fractionation and isolation carried out on the methanol extract of Adonis amurensis led to the identification of three compounds, namely cymarin, cymarol, and cymarilic acid. Amongst the three compounds, cymarilic acid was isolated from this plant for the first time. This compound showed no significant cytotoxicity against tumor cell lines but was found to be strongly inhibitory toward tube formation induced by human umbilical venous endothelial (HUVE) cells. Cymarin and cymarol exhibited potent cytotoxicity against a human solid tumor cell line A549 (human lung carcinoma), while being inactive on murine leukemic cells (L1210).
College of Pharmacy, Chungnam National University, Taejon 305-764, South Korea.
Young-Jae
YJ
You
Yong
Y
Kim
Nguyen-Hai
NH
Nam
Byung-Zun
BZ
Ahn
D031843:Adonis; D000465:Algorithms; D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002298:Cardenolides / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D003529:Cymarine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D051379:Mice; D015394:Molecular Structure; D010936:Plant Extracts / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014471:Umbilical Veins / Q000166:cytology / Q000187:drug effects
D020533:Angiogenesis Inhibitors; D000972:Antineoplastic Agents, Phytogenic; D002298:Cardenolides; D010936:Plant Extracts; C475937:cymarilic acid; C000546:cymarol; D003529:Cymarine