[Ethanol-induced apoptosis in human HL-60 cells].
The purpose of this study was to examine the effect of ethanol on apoptosis of human promyelocytic leukemia cell line HL-60 cells and search for a possible reason for reduced PMNs, monocytes, lymphocytes in the blood of alcoholics. Human promyelocytic leukemia cell line HL-60 cells was used as a model of apoptosis, with ethanol being the interfering factor. Qualitative and quantitative detection was done by both electron microscopy and flow cytometry. Compared with the control treatment of HL-60 cells with ethanol at 50, 100, 150, 200, 250 mmol/L for 3 days increased apoptotic cells significantly (P < 0.01), and apoptosis was dose-dependent for ethanol. It suggest that ethanol can induce apoptosis of HL-60 cells. So as far as reduced PMNs, monocytes, phagocytes and lymphocytes of alcoholics are concerned, ethanol-induced apoptosis may well be an important reason.
Medical Analysis Center, Beijing Medical University, Beijing 100083, China.
Z
Z
Guan
Y
Y
Li
S
S
Wang
Y
Y
Yang
D017209:Apoptosis / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D000431:Ethanol / Q000633:toxicity*; D018922:HL-60 Cells / Q000166:cytology*; D006801:Humans
D000431:Ethanol