Phylogenetic analysis of the family Thermaceae with an emphasis on signature position and secondary structure of 16S rRNA.
The sequences of the 16S rRNA genes from 38 strains of the family Thermaceae were compared by alignment analysis. The genus-specific and species-specific base substitutions or base deletions (signature positions) were found in three hypervariable regions (in the helices 6, 10 and 17). The differentiation of secondary structures of the high variable regions in the 5' end (38-497) containing several signature positions further supported the concept. Based on the comparisons of the secondary structures in the segments of 16S rRNAs, a key to the species of the family Thermaceae was proposed.
Department of Biotechnology, Kunming University of Science and Technology (KUST), Kunming, PR China.
Chaoyin
C
Chen
Shenglan
S
Zhao
Kunlong
K
Ben
D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D006094:Gram-Positive Bacteria / Q000145:classification* / Q000235:genetics; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000737:chemistry / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000737:chemistry* / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; D012336:RNA, Ribosomal, 16S