Antioxidative compounds from Crotalaria sessiliflora.
Seven antioxidative compounds were isolated from the EtOAc extract of the aerial part of C. sessiliflora (Japanese name, tanukimame) by activity-guided fractionation with 2,2-diphenyl-1-picrylhydrazyl (DPPH). Among the isolated compounds, hydroxyeucomic acid showed the strongest free radical-scavenging activity, which was almost identical to that of epigallocatechin gallate, against DPPH. Orientin and isoorientin showed strong anti-peroxidative activities toward linoleic acid and protective effects against the bactericidal action of the tert-butyl peroxyl radical. Their activities were nearly equal to that of epigallocatechin gallate.
Institute of Applied Biochemistry, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan.
Abdul
A
Mun'im
Osamu
O
Negishi
Tetsuo
T
Ozawa
D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D005591:Chemical Fractionation / Q000379:methods; D029864:Crotalaria / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000032:analysis / Q000494:pharmacology; D005609:Free Radicals / Q000037:antagonists & inhibitors; D006834:Hydrazines / Q000737:chemistry; D019787:Linoleic Acid / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D010084:Oxidation-Reduction; D010851:Picrates; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D020122:tert-Butylhydroperoxide / Q000737:chemistry
D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D005609:Free Radicals; D006834:Hydrazines; D010851:Picrates; D010936:Plant Extracts; D002392:Catechin; D020122:tert-Butylhydroperoxide; D019787:Linoleic Acid; C045651:epigallocatechin gallate; C004931:1,1-diphenyl-2-picrylhydrazyl