P system antigenic determiners expression in Ascaris lumbricoides.
The P System antigens have been detected in numerous parasites, bacteria and viruses, nevertheless the clinical significance is still unknown. The aim was to study the presence of P1 antigenic determiners in A. lumbricoides extracts by means of the use of 6 different monoclonal antibodies of well-known concentrations and Ig class. We worked with 14 A. lumbricoides extracts. Inhibition Agglutination Test was made in a bromelin enzymatic medium and 4 degrees C temperature. Titre, Score and Sensitivity Parameter were determined for each monoclonal antibody against red cells suspension used as revealing system. Ten extracts inhibited the agglutination of all anti P1 monoclonal antibodies. The 4 remaining extracts only inhibited the agglutination of some of them. It is demonstrated that the extracts have P1 activity. This activity is independent of titre, Score, Sensitivity Parameter, concentration and Ig class and it depends on the epitope at which the monoclonal antibody is directed.
Laboratorio de Parasitología, Laboratorio de Inmunohematología, Facultad de Cs. Bioq. y Farm. U.N.R Suipacha 531-2000 Rosario, Argentina.
Patricia
P
Ponce de León
Juana
J
Valverde
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D017164:Ascaris lumbricoides / Q000276:immunology*; D000939:Epitopes / Q000032:analysis*; D006385:Hemagglutination Inhibition Tests; D010127:P Blood-Group System / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D010127:P Blood-Group System