Cytoprotective activity of Amla (Emblica officinalis) against chromium (VI) induced oxidative injury in murine macrophages.
The cytoprotective and immunomodulating properties of Emblica officinalis (Amla) against chromium (VI) induced oxidative damage are reported. Chromium (VI) at 1 micro g/mL concentration was highly cytotoxic. It enhanced free radical production and decreased reduced glutathione (GSH) levels and glutathione peroxidase (GPx) activity in macrophages. The presence of Amla resulted in an enhanced cell survival, decreased free radical production and higher antioxidant levels similar to that of control cells. Further, chromium (VI) treatment resulted in decreased phagocytosis and gamma-interferon (gamma-IFN) production while Amla inhibited chromium induced immunosuppression and restored both phagocytosis and gamma-IFN production by macrophages significantly.
Defence Institute of Physiology and Allied Sciences, Timarpur, Lucknow Road, Delhi-110054, India. sairam64@rediffmail.com
M
M
Sai Ram
D
D
Neetu
P
P
Deepti
M
M
Vandana
G
G
Ilavazhagan
Devendra
D
Kumar
W
W
Selvamurthy
D000818:Animals; D002857:Chromium / Q000494:pharmacology; D007166:Immunosuppressive Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D007371:Interferon-gamma / Q000096:biosynthesis; D007477:Ions; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects; D029842:Phyllanthus emblica; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D020011:Protective Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use
D007166:Immunosuppressive Agents; D007477:Ions; D010936:Plant Extracts; D020011:Protective Agents; D002857:Chromium; D007371:Interferon-gamma