Acid Glycohydrolases in Rat Spermatocytes, Spermatids and Spermatozoa: Enzyme Activities, Biosynthesis and Immunolocalization.
Mammalian sperm acrosome contains several glycohydrolases thought to aid in the dispersion and digestion of vestments surrounding the egg. In this study, we have used multiple approaches to examine the origin of acrosome-associated glycohdyrdolases. Mixed spermatogenic cells, prepared from rat testis, were separated by unit gravity sedimentation. The purified germ cells (spermatocytes [SP], round spermatids [RS], and elongated/condensed spermatids [E/CS]) contained several glycohydrolase activities. Metabolic labeling in the cell culture, immunoprecipitation, and autoradiographic approaches revealed that beta-D-galactosidase was synthesized in SP and RS in 88/90 kDa forms which undergo processing in a cell-specific manner. Immunohistochemical approaches demonstrated that the enzyme was localized in Golgi membranes/vesicles, and lysosome-like structures in SP and RS, and forming/formed acrosome of E/CS.
Abou-Haila
Aida
A
Laboratoire d'Embryologie et Biologie de la Reproduction, UFR Biomédicale. Universite René Descartes, 45 rue des Saints-Pères, 75270 Paris, Cedex 06. France.Departments of Obstetrics & Gynecology and Cell Biology. Vanderbilt University School of Medicine, Room D-3243 MCN, Nashville, TN 37232-2633. USA. /322-4358. daulat.tulsiani@mcmail.vanderbilt.edu
Tulsiani
Daulat RP
DR