Potential for proteomic profiling of Helicobacter pylori and other Helicobacter spp. using a ProteinChip array.
The Helicobacter genus is associated with a wide spectrum of pathologies in the gastrointestinal tract. However, in contrast to Helicobacter pylori, few data are available regarding proteomic characteristics of enterohepatic helicobacters. Proteomic analysis of this genus has predominantly utilised two-dimensional gel electrophoresis methodology. In the present study we applied an innovative technique using ProteinChip arrays coupled with surface-enhanced laser desorption/ionisation time of flight mass spectroscopy to accurately assess the M(r) of proteins for comparative proteomic profiling. We analysed binding of outer membrane fractions to a weak cation exchange array for strains of H. pylori from culture collections and compared these profiles to fresh clinical isolates. In addition, we analysed profiles from Helicobacter pullorum, Helicobacter bilis and 'Helicobacter sp. flexispira'. The system proved rapid, accurate and reproducible. Distinct specific profiles for all the strains studied were identified. However, strains from culture collections that have undergone numerous subcultures had almost identical profiles. In contrast, profiles from fresh clinical isolates were markedly different. Moreover, certain features of the profiles from the enterohepatic species were conserved.
Department of Medical Microbiology, Dermatology and Infection, Lund University, Sölvegatan 23, SE-22362 Lund, Sweden.
Sean O
SO
Hynes
Jim
J
McGuire
Torkel
T
Wadström
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D002411:Cation Exchange Resins; D003470:Culture Media; D004285:Dogs; D016998:Helicobacter / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification; D016481:Helicobacter Infections / Q000382:microbiology; D016480:Helicobacter pylori / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification; D006801:Humans; D007231:Infant, Newborn; D040081:Protein Array Analysis; D020543:Proteome / Q000737:chemistry*; D012692:Serial Passage; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D001425:Bacterial Outer Membrane Proteins; D002411:Cation Exchange Resins; D003470:Culture Media; D020543:Proteome