[Effect on proliferation and apoptosis of human lingual carcinoma cells cotransfected by bax and p53 genes].
OBJECTIVE
To investigate the effects on proliferation and apoptosis of human cancerous cells cotransfected by bax apoptosis-inducing gene and p53 tumor suppressor gene.


METHODS
The chimeric gene pSV-CIP-bax-CAT was constructed in which bax gene was flanked upstream by a 217 bp fragment(+822(-)+1093) of the first intron and a 317 bp promoter fragment of human a 1(I) collagen gene. Human lingual carcinoma cell line Tca 8113 (LCC) in culture was respectively transfected with pSV-CIP-bax-CAT, and cotransfected with pSV-p53-CAT and pSV-CIP-bax-CAT by using the transfection reagent DOSPER.


RESULTS
Immuno-slot blot and ELISA demonstrated that the expression of bax and p53 genes increased remarkably in the transfected and cotransfected LCC, compared with the controls (LCC transfected with pSV-CIP-CAT and LCC). MTT colorimetric assay, TUNEL fluorenscence microscopy and flow cytometry showed that foreign bax or p53 gene inhibited the LCC growth and induced apoptosis (23.9% and 26.1% of inhibitory rate by bax gene and p53 plus bax genes respectively).


CONCLUSIONS
The ectopic expression of the bax gene in the LCC promoted by the cis-acting elements of human a1 (I)collagen gene has obviously synergistic effect on the proliferation and apoptosis of the LCC contransfected with p53 gene plus bax gene for 48 hours.
Institute of Biochemistry and Molecular Biology, School of Basic Medical Sciences, WCUMS, Chengdu 610041, China.
Y
Y
Ma
J
J
Chen
J
J
Gao
W
W
Peng
R
R
Wang
S
S
Yang
D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D002455:Cell Division; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D019253:Proto-Oncogene Proteins c-bcl-2; D014062:Tongue Neoplasms / Q000235:genetics* / Q000473:pathology; D014162:Transfection; D014407:Tumor Cells, Cultured; D051028:bcl-2-Associated X Protein
C494826:BAX protein, human; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein