Crosstalk between neurokinin receptors is relevant to hematopoietic regulation: cloning and characterization of neurokinin-2 promoter.
Neurokinin (NK)-1 and NK-2 receptors regulate hematopoiesis by interacting with neurotransmitters that belong to the tachykinin. This report studies the relationship between NK-1 and NK-2 in primary human bone marrow (BM) stroma, which supports hematopoiesis. Use of NK receptor antagonists and deficient stromal cells indicate that the neurotransmitter, substance P (SP), could exert dual hematopoietic effects (inhibitory or stimulatory), depending on the interacting receptor and crosstalk between NK-1 and NK-2. Cloning and identification of the minimal promoter for NK-2 and comparison with NK-1 promoter showed that the hematopoietic functions of NK receptors involve receptor crosstalk and the particular cytokine (IL-3, GM-CSF, TGF-beta or IL-1alpha). Crosstalk between NK-1 and NK-2 adds to communication within neural-hematopoietic axis.
Department of Medicine, UMDNJ-New Jersey Medical School, MSB, Rm. E-579, 185 South Orange Avenue, Newark, NJ 07103, USA.
Persis S
PS
Bandari
Jing
J
Qian
Hyun S
HS
Oh
Julius A
JA
Potian
Ghassan
G
Yehia
Jonathan S
JS
Harrison
Pranela
P
Rameshwar
D020121:5' Untranslated Regions / Q000187:drug effects; D001483:Base Sequence; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D003001:Cloning, Molecular / Q000379:methods; D016207:Cytokines / Q000494:pharmacology; D015536:Down-Regulation / Q000187:drug effects; D017353:Gene Deletion; D006410:Hematopoiesis / Q000235:genetics / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D064729:Neurokinin-1 Receptor Antagonists; D011401:Promoter Regions, Genetic / Q000187:drug effects / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D020239:Receptor Cross-Talk / Q000502:physiology*; D018040:Receptors, Neurokinin-1 / Q000235:genetics / Q000502:physiology*; D018041:Receptors, Neurokinin-2 / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D017154:Stromal Cells / Q000187:drug effects / Q000378:metabolism; D013373:Substance P / Q000494:pharmacology; D014162:Transfection; D015854:Up-Regulation / Q000187:drug effects
D020121:5' Untranslated Regions; D016207:Cytokines; D064729:Neurokinin-1 Receptor Antagonists; D012333:RNA, Messenger; D018040:Receptors, Neurokinin-1; D018041:Receptors, Neurokinin-2; D013373:Substance P