Influence of industrial processing on orange juice flavanone solubility and transformation to chalcones under gastrointestinal conditions.
Orange juice manufactured at industrial scale was subjected to digestion under in vitro gastrointestinal conditions (pH, temperature, and enzyme and chemical conditions) to evaluate the influence of individual industrial processing treatments on flavanone solubility, stability, and ability to permeate through a membrane under simulated physiological conditions. Four industrial processes including squeezing, standard pasteurization, concentration, and freezing were evaluated. Hand squeezing was compared with industrial squeezing. After in vitro gastrointestinal digestion of the orange juices, the flavanones able to permeate through a dialysis membrane, and those remaining in the retentate were evaluated by HPLC as were those present in the insoluble fraction. In all of the assayed orange juices, a high content of precipitated chalcones ( approximately 70% of the total flavanones) was formed under the physiological conditions of the gastrointestinal tract. Hand squeezing provided a higher concentration of flavanones in the permeated fraction and lower transformation to chalcones than industrial squeezing. Standard pasteurization did not influence the solubility and permeability of the orange juice flavanones and chalcones. Industrial concentration did not affect the amount of flavanones able to permeate but decreased the chalcones produced. Juices produced from frozen orange juice contained considerably smaller amounts of both soluble flavanones and insoluble chalcones.
Research Group on Quality, Safety and Bioactivity of Plant Foods, Departamento Ciencia y Tecnología de Alimentos, CEBAS-CSIC, P.O. Box 4195, 30080 Murcia, Spain.
Angel
A
Gil-Izquierdo
María Isabel
MI
Gil
Francisco Abraham
FA
Tomas-Barberan
Federico
F
Ferreres
D001628:Beverages / Q000032:analysis*; D002599:Chalcone / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002957:Citrus / Q000737:chemistry*; D003460:Crystallization; D004063:Digestion; D004064:Digestive System / Q000378:metabolism*; D004355:Drug Stability; D044950:Flavanones; D005419:Flavonoids / Q000737:chemistry* / Q000378:metabolism*; D005511:Food Handling; D005615:Freezing; D006569:Hesperidin / Q000032:analysis; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D012995:Solubility
D044950:Flavanones; D005419:Flavonoids; D002599:Chalcone; D006569:Hesperidin; C028610:flavanone