[Relationship between arbekacin-susceptibility and aminoglycoside-resistant gene of methicillin-resistant Staphylococcus aureus (MRSA)].
The susceptibility to arbekacin (ABK) of methicillin-resistant Staphylococcus aureus (MRSA) was investigated to find out how it related to aac(6')/aph(2") gene. In 49 isolates of MRSA for which MIC of ABK ranged from 0.125 to 64 micrograms/ml, the MICs of ABK for 38 strains carrying aac(6')/aph(2") gene were widely distributed from 0.25 to 64, whereas those for 11 strains without that gene were all < or = 0.5 microgram/ml. Residual rate of ABK activity was higher than that of gentamicin after the reaction with each crude enzyme preparation extracted from 3 isolates of MRSA, carrying aac(6')/aph(2") and aad(4',4") genes. Furthermore, 97 strains of MRSA isolated at Kanagawa prefecture in Japan in 1999 were all sensitive to ABK, although 28 strains of them carried aac(6')/aph(2") gene. These results showed that ABK resistance was not necessarily related to carrying aac(6')/aph(2") gene in clinical isolates of MRSA.
Pharmaceutical Research Center, Meiji Seika Kaisha, Ltd., 760 Morooka-cho, Kohoku-ku, Yokohama, Kanagawa 222-8567, Japan.
Makiko
M
Tabata
Masaki
M
Shimizu
Minako
M
Araake
Hiroshi
H
Ogawa
D000123:Acetyltransferases; D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003982:Dibekacin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D016106:Methicillin Resistance; D017853:Phosphotransferases (Alcohol Group Acceptor); D013211:Staphylococcus aureus / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics*
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D003982:Dibekacin; C108453:6'-aminoglycoside acetyltransferase-2''-aminoglycoside phosphotransferase; D000123:Acetyltransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C031986:arbekacin