O/W emulsification for the self-aggregation and nanoparticle formation of linoleic acid-modified chitosan in the aqueous system.
Chitosan was modified by coupling with linoleic acid through the 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide-mediated reaction to increase its amphipathicity for improved emulsification. The micelle formation of linoleic acid-modified chitosan in the 0.1 M acetic acid solution was enhanced by O/W emulsification with methylene chloride, an oil phase. The fluorescence spectra indicate that without emulsification the self-aggregation of LA-chitosan occurred at the concentration of 1.0 g/L or above, and with emulsification, self-aggregation was greatly enhanced followed by a stable micelle formation at 2.0 g/L. The addition of 1 M sodium chloride promoted the self-aggregation of LA-chitosan molecules both with and without emulsification. The micelles of LA-chitosan formed nanosize particles ranging from 200 to 600 nm. The LA-chitosan nanoparticles encapsulated the lipid soluble model compound, retinal acetate, with 50% efficiency.
Chen
Xi-Guang
XG
The Life Science College, Ocean University of Qingdao, Qingdao 266003, People's Republic of China.
Lee
Chong M
CM
Park
Hyun-Jin
HJ
D019342:Acetic Acid; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry*; D048271:Chitosan; D004655:Emulsions / Q000737:chemistry*; D019787:Linoleic Acid / Q000737:chemistry*; D008823:Micelles; D010316:Particle Size; D012965:Sodium Chloride / Q000494:pharmacology; D012996:Solutions; D013050:Spectrometry, Fluorescence; D014867:Water / Q000737:chemistry*
D004655:Emulsions; D008823:Micelles; D012996:Solutions; D014867:Water; D002686:Chitin; D012965:Sodium Chloride; D048271:Chitosan; D019787:Linoleic Acid; D019342:Acetic Acid