The regulation of bone resorption in tooth formation and eruption processes in mouse alveolar crest devoid of cathepsin k.
Osteoclastic bone resorption has recently been implicated in the tooth formation and eruption in alveolar bone. Cathepsin K (CK) is a cysteine proteinase expressed predominantly in osteoclasts and is believed to play a critical role in degradation of bone matrix proteins. Here we present evidence that the alveolar bone resorption is essential for the tooth formation and that eruption proceeds normally in CK-deficient (CK-/-) mice. Radiographic and histological analyses revealed that the alveolar bone from these animals had no significant abnormalities during the tooth development between 5 and 28 days after birth. The tooth crown was normally erupted through the alveolar bone layer at 28 days after birth. The number of tartrate-resistant acid phosphatase-positive multinuclear cells in the alveolar bone around the tooth germ was apparently increased in 5-day-old CK-/- mice compared with age-matched littermates. More important, however, the immunohistochemical localization of matrix metalloproteinase-9 (MMP-9) was clearly increased in the CK-/- osteoclasts. In contrast, no significant difference in the immunoreactivity for cathepsin D was observed between the CK-/- osteoclasts and the wild-type ones. These results indicate that CK-/- osteoclasts are fully differentiated and are capable of degrading the organic phase of alveolar bone during the tooth formation and eruption, which may result from the compensatory action by MMP-9 increasingly expressed in the osteoclasts.
Department of Orthodontics, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki 852-8588, Japan.
Masayo
M
Okaji
Hideaki
H
Sakai
Eiko
E
Sakai
Mitsue
M
Shibata
Fumio
F
Hashimoto
Yasuhiro
Y
Kobayashi
Noriaki
N
Yoshida
Kuniaki
K
Okamoto
Kenji
K
Yamamoto
Yuzo
Y
Kato
D016301:Alveolar Bone Loss / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001862:Bone Resorption / Q000235:genetics*; D056657:Cathepsin K; D002403:Cathepsins / Q000172:deficiency* / Q000235:genetics* / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010010:Osteoclasts / Q000378:metabolism; D014070:Tooth / Q000254:growth & development* / Q000378:metabolism* / Q000502:physiology; D014078:Tooth Eruption / Q000235:genetics* / Q000502:physiology
D002403:Cathepsins; D056657:Cathepsin K; C534132:Ctsk protein, mouse