Liquid chromatography-fluorescence detection for simultaneous analysis of sulfonamide residues in honey.
A rapid, accurate LC analytical method has been developed for determination of eight sulfonamides (sulfacetamide, sulfapyridine, sulfamerazine, sulfamethoxypyridazine, sulfameter, sulfachloropyridazine, sulfamethoxazole and sulfadimethoxine) in honey. The sample was dissolved in phosphoric acid solution (pH 2). After filtration, the sample solution was cleaned by use of two solid-phase extraction (SPE) cartridges-an aromatic sulfonic cation-exchange cartridge and an Oasis HLB cartridge. The eight sulfonamides were then derivatized with fluorescamine and the derivatives were determined by LC with fluorescence detection at excitation and emission wavelengths of 405 and 495 nm, respectively. Average recoveries at three fortification levels in the range 0.02-0.50 mg kg(-1) in twelve different kinds of honey were 73.5-94.1% with coefficients of variation of 4.35-16.60%. The limit of detection (LOD) was 0.002 mg kg(-1) for sulfacetamide, sulfapyridine, sulfamerazine, and sulfamethoxypyridazine; that for sulfameter, sulfachloropyridazine, sulfamethoxazole and sulfadimethoxine was 0.005 mg kg(-1). The limit of quantitation (LOQ) was 0.005 mg kg(-1) for sulfacetamide, sulfapyridine, sulfamerazine, and sulfamethoxypyridazine; that for sulfameter, sulfachloropyridazine, sulfamethoxazole, and sulfadimethoxine was 0.010 mg kg(-1). The method is suitable for determination of multiresidue sulfonamides in the various kinds of honey.
Pang
Guo-Fang
GF
Qinhuangdao Entry-Exit Inspection and Quarantine Bureau, 39 Haibin Rd, PC 066002, Qinhuangdao, Hebei, P. R. China. gfpang@heinfo.net
Cao
Yan-Zhong
YZ
Fan
Chun-Lin
CL
Zhang
Jin-Jie
JJ
Li
Xue-Min
XM
Li
Zeng-Yin
ZY
Jia
Guang-Qun
GQ
D000890:Anti-Infective Agents / Q000302:isolation & purification*; D002853:Chromatography, Liquid; D005453:Fluorescence; D005504:Food Analysis / Q000379:methods*; D006722:Honey / Q000032:analysis*; D007202:Indicators and Reagents; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013449:Sulfonamides / Q000302:isolation & purification*
D000890:Anti-Infective Agents; D007202:Indicators and Reagents; D013449:Sulfonamides