Effect of docosahexaenoic acid and ascorbate on peroxidation of retinal membranes of ODS rats.
Mutant male osteogenic disorder Shionogi (ODS) rats, unable to synthesize ascorbic acid, were fed diets containing a high content of docosahexaenoic acid (DHA) and different amounts of ascorbic acid, to study the effect of DHA on peroxidative susceptibility of the retina and possible antioxidant action of ascorbic acid. ODS rats were fed from 7 weeks of age with diets containing high DHA (6.4% of total energy). A control group received a diet high in linoleic acid. The diets also contained varying amounts of ascorbic acid. Fatty acid compositions and phospholipid hydroperoxides in rod outer segment (ROS) membranes, and retinal ascorbic acid were analyzed. DHA in ROS membranes was significantly increased in rats fed high DHA, compared with the linoleic acid diet. Levels of phospholipid hydroperoxides in the DHA-fed rats were significantly higher than the linoleic acid-fed rats. Ascorbic acid supplementation did not suppress the phospholipid hydroperoxide levels after a high DHA diet, even when the supplement increased the content of retinal ascorbic acid. In conclusion, high DHA feeding induced a marked increase of phospholipid hydroperoxides in ROS membranes of ODS rats. Supplementation of ascorbic acid did not reverse this increase.
Wang
Jin-Ye
JY
Division of Food Science, Independently Administered Institution, National Institute of Health and Nutrition, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8636, Japan. jywang@pub.sioc.ac.cn
Sekine
Seiji
S
Saito
Morio
M
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001205:Ascorbic Acid / Q000378:metabolism / Q000494:pharmacology*; D002462:Cell Membrane / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004281:Docosahexaenoic Acids / Q000494:pharmacology*; D005227:Fatty Acids / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D019787:Linoleic Acid / Q000494:pharmacology; D010545:Peroxides / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism; D051381:Rats; D012160:Retina / Q000187:drug effects*; D012374:Rod Cell Outer Segment / Q000187:drug effects; D013997:Time Factors
D000975:Antioxidants; D005227:Fatty Acids; D010545:Peroxides; D010713:Phosphatidylcholines; D004281:Docosahexaenoic Acids; D019787:Linoleic Acid; D006861:Hydrogen Peroxide; D001205:Ascorbic Acid