Endotoxic and immunobiological activities of a chemically synthesized lipid A of Helicobacter pylori strain 206-1.
A synthetic lipid A of Helicobacter pylori strain 206-1 (compound HP206-1), which is similar to its natural lipid A, exhibited no or very low endotoxic activities as compared to Escherichia coli-type synthetic lipid A (compound 506). Furthermore, compound HP206-1 as well as its natural lipid A demonstrated no or very low mitogenic responses in murine spleen cell. On the other hand, compound HP206-1 showed a weaker but significant production of interleukin-8 in a gastric cancer cell line, MKN-1, in comparison with compound 506. Furthermore, compound HP206-1 exhibited induction of tumor necrosis factor-alpha production in human peripheral blood mononuclear cells and the cytokine production was clearly inhibited by mouse anti-human Toll-like receptor (TLR) 4 monoclonal antibody HTA125. Our findings indicate that the chemically synthesized lipid A, mimicking the natural lipid A portion of lipopolysaccharide from H. pylori strain 206-1, has a low endotoxic potency and immunobiological activities, and is recognized by TLR4.
Department of Oral Microbiology, Asahi University School of Dentistry, 1851-1 Hozumi, Hozumi-cho, Motosu-gun, Gifu 501-0296, Japan. tomo527@dent.asahi-u.ac.jp
Tomohiko
T
Ogawa
Yasuyuki
Y
Asai
Yasuhiro
Y
Sakai
Masato
M
Oikawa
Koichi
K
Fukase
Yasuo
Y
Suda
Shoichi
S
Kusumoto
Toshihide
T
Tamura
D000818:Animals; D016480:Helicobacter pylori / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D016209:Interleukin-8 / Q000276:immunology / Q000378:metabolism; D007928:Lethal Dose 50; D008050:Lipid A / Q000138:chemical synthesis / Q000276:immunology / Q000378:metabolism / Q000633:toxicity*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D011817:Rabbits; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology / Q000378:metabolism
D016209:Interleukin-8; D008050:Lipid A; D014409:Tumor Necrosis Factor-alpha