Combined histomorphometric and gene-expression profiling applied to toxicology.
We have developed a unique methodology for the combined analysis of histomorphometric and gene-expression profiles amenable to intensive data mining and multisample comparison for a comprehensive approach to toxicology. This hybrid technology, termed extensible morphometric relational gene-expression analysis (EMeRGE), is applied in a toxicological study of time-varied vehicle- and carbon-tetrachloride (CCl4)-treated rats, and demonstrates correlations between specific genes and tissue structures that can augment interpretation of biological observations and diagnosis.
Tissue Informatics Inc, 711 Bingham Street, Suite 200, Pittsburgh, PA 15203, USA. akriete@tissueinformatics.com
Andres
A
Kriete
Mary K
MK
Anderson
Brad
B
Love
John
J
Freund
James J
JJ
Caffrey
M Brook
MB
Young
Timothy J
TJ
Sendera
Scott R
SR
Magnuson
J Mark
JM
Braughler
D000818:Animals; D002251:Carbon Tetrachloride / Q000633:toxicity; D056486:Chemical and Drug Induced Liver Injury; D003627:Data Interpretation, Statistical; D020869:Gene Expression Profiling; D006651:Histocytochemistry / Q000379:methods; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008107:Liver Diseases / Q000235:genetics / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012313:RNA / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014116:Toxicology / Q000379:methods*
D012313:RNA; D002251:Carbon Tetrachloride