Analysis of epithelial-myoepithelial carcinoma based on the establishment of a novel cell line.
Epithelial-myoepithelial carcinoma of the salivary gland is a rare, low-grade, neoplasm, composed of ductal and myoepithelial cells. We present two novel cell lines, which have been characterised by immunofluorescence, derived from an epithelial-myoepithelial carcinoma of the parotid gland. A resected mass of the parotid gland was diagnosed as an epithelial-myoepithelial carcinoma by routine histological examination. Part of the specimen was labelled with a panel of antibodies confirming the tumour type. The other part was finely minced and the explants were incubated in DMEM supplemented with penicillin and streptomycin, at 37 degrees C in a humidified 5% CO(2) atmosphere. Two cell types were identified by immunofluorescence-a small cobblestone cell, positive for AE1/AE3 and p53, and a polyhedral cell, positive for vimentin, smooth muscle markers and S-100. Herein two cell lines are presented in order to open up possibilities of new studies and a discussion of the events that culminate in this bimodal neoplasm is also performed.
Mantesso
A
A
Dental Research Institute, São Leopoldo Mandic-Rua Abolição 1827, Swift, SP, CEP 13045-610, Campinas, Brazil. amantesso@hotmail.com
Loducca
S V L
SV
Jaeger
R G
RG
Décio
S P J
SP
Araújo
V C
VC
D000328:Adult; D002277:Carcinoma / Q000737:chemistry / Q000473:pathology*; D018929:Cell Culture Techniques / Q000379:methods; D005260:Female; D006801:Humans; D007633:Keratins / Q000032:analysis; D009363:Neoplasm Proteins / Q000032:analysis; D009687:Nuclear Proteins; D010307:Parotid Neoplasms / Q000737:chemistry / Q000473:pathology*; D011518:Proto-Oncogene Proteins / Q000032:analysis; D051736:Proto-Oncogene Proteins c-mdm2; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000473:pathology*; D016159:Tumor Suppressor Protein p53 / Q000032:analysis
D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; D016159:Tumor Suppressor Protein p53; D007633:Keratins; C497206:MDM2 protein, human; D051736:Proto-Oncogene Proteins c-mdm2