Calcium-independent phospholipase A2 is required for lysozyme secretion in U937 promonocytes.
As a part of their surveillance functions in the immune system, monocytes/macrophages secrete large amounts of the bactericidal enzyme lysozyme to the extracellular medium. We report here that lysozyme secretion in activated U937 promonocytes depends on a functional calcium-independent phospholipase A(2) (iPLA(2)). Inhibition of the enzyme by bromoenol lactone or by treatment with a specific antisense oligonucleotide results in a diminished capacity of the cells to secrete lysozyme to the extracellular medium. Calcium-independent PLA(2) is largely responsible for the maintenance of the steady state of lysophosphatidylcholine (lysoPC) levels within the cells, as manifested by the marked decrease in the levels of this metabolite in cells deficient in iPLA(2) activity. Reconstitution experiments reveal that lysoPC efficiently restores lysozyme secretion in iPLA(2)-deficient cells, whereas other lysophospholipids, including lysophosphatidic acid, lysophosphatidylserine, and lysophosphatidylethanolamine, are without effect. Arachidonic acid mobilization in activated U937 cells is under control of cytosolic phospholipase A(2) (cPLA(2)). Selective inhibition of cPLA(2) results in a complete abrogation of the arachidonate mobilization response, but has no effect on lysozyme secretion. These results identify iPLA(2)-mediated lysoPC production as a necessary component of the molecular machinery leading to lysozyme secretion in U937 cells and rule out a role for cPLA(2) in the response. Collectively, the results demonstrate distinct roles in inflammatory cell signaling for these two intracellular phospholipases.
Balboa
María A
MA
Institute of Molecular Biology and Genetics, University of Valladolid School of Medicine, Valladolid, Spain.
Sáez
Yolanda
Y
Balsinde
Jesús
J
D016718:Arachidonic Acid / Q000378:metabolism / Q000502:physiology; D002118:Calcium / Q000502:physiology; D002458:Cell Fractionation; D003600:Cytosol / Q000201:enzymology; D004789:Enzyme Activation / Q000502:physiology; D054513:Group IV Phospholipases A2; D054522:Group VI Phospholipases A2; D006801:Humans; D008244:Lysophosphatidylcholines / Q000037:antagonists & inhibitors / Q000378:metabolism; D009000:Monocytes / Q000201:enzymology* / Q000378:metabolism*; D009113:Muramidase / Q000378:metabolism*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D010741:Phospholipases A / Q000037:antagonists & inhibitors / Q000172:deficiency / Q000502:physiology*; D054467:Phospholipases A2; D013234:Stem Cells / Q000201:enzymology* / Q000378:metabolism*; D020298:U937 Cells
D008244:Lysophosphatidylcholines; D016376:Oligonucleotides, Antisense; D016718:Arachidonic Acid; D010741:Phospholipases A; D054513:Group IV Phospholipases A2; D054522:Group VI Phospholipases A2; C516389:PLA2G4A protein, human; C516475:PLA2G6 protein, human; D054467:Phospholipases A2; D009113:Muramidase; D002118:Calcium