Apoptosis of endothelial cells may be mediated by genes of peroxisome proliferator-activated receptor gamma 1 (PPARgamma 1) and PPARalpha genes.
Apoptosis in human umbilical vein endothelial cells (HUVECs) was prevented by transfection with the gene for the human full-length peroxisome proliferator-activated receptor alpha (PPARalpha), or acyl-coenzyme A synthetase (AcylCS) into HUVECs. In contrast, ligands/activators of PPARgamma 1 induced apoptosis by a cytochrome c-dependent mechanism in HUVECs transfected with human full-length PPARgamma 1, but not in hepatocytes. Co-transfection of PPARgamma 1 and PPARalpha protected the HUVEC apoptosis. The results suggest that the apoptosis of endothelial cells may be mediated by genes of PPARgamma1 and PPARalpha.
Fourth Department of Medicine, Saitama Medical School, 38 Morohongo, Moroyama, Iruma-gun, Saitama 350-0495, Japan. i1901018@saitama-med.ac.jp
Ikuo
I
Inoue
Kenji
K
Hayashi
Fumiharu
F
Yagasaki
Koh-ichi
K
Nakamura
Toshiyuki
T
Matsunaga
Haiyuan
H
Xu
Koh-ichi
K
Inukai
Takuya
T
Awata
Tsugikazu
T
Komoda
Shigehiro
S
Katayama
D017209:Apoptosis / Q000235:genetics* / Q000502:physiology; D002478:Cells, Cultured; D003066:Coenzyme A Ligases / Q000235:genetics / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism; D006801:Humans; D050356:Lipid Metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D014471:Umbilical Veins
D029968:Escherichia coli Proteins; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors; D003066:Coenzyme A Ligases; C085934:acyl-coenzyme A synthetase, E coli