[The simultaneous isolation of outer hair cells and Deiters' cells from the cochlea of the guinea pig].
OBJECTIVE
To investigate the simultaneous method of isolating outer hair cells and Deiters' cells from the organ of Corti of the guinea-pig cochlea.


METHODS
The organ of Corti was dissected from the pigmental guinea pig cochlea, and then treated with papain.


RESULTS
A large number of living outer hair cells and Deiters' cells were obtained synchronously from the guinea pig.


CONCLUSIONS
The key to success in isolating outer hair cells and Deiters' cells from the cochlea is to know very well the anatomical characteristics of the cochlea and keep the contact the organ of Corti.
Chang
Qing
Q
Department of Otolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022.
Gong
Shusheng
S
Tang
Ming
M
Hescheler
J
J
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D003051:Cochlea / Q000166:cytology*; D005260:Female; D006168:Guinea Pigs; D018072:Hair Cells, Auditory, Outer / Q000166:cytology*; D066298:In Vitro Techniques; D008297:Male; D003689:Vestibular Nucleus, Lateral / Q000166:cytology*