Continuous production of butanol from starch-based packing peanuts.
Acetone, butanol, ethanol (ABE, or solvents) were produced from starch-based packing peanuts in batch and continuous reactors. In a batch reactor, 18.9 g/L of total ABE was produced from 80 g/L packing peanuts in 110 h of fermentation. The initial and final starch concentrations were 69.6 and 11.1 g/L, respectively. In this fermentation, ABE yield and productivity of 0.32 and 0.17 g/(L h) were obtained, respectively. Compared to the batch fermentation, continuous fermentation of 40 g/L of starchbased packing peanuts in P2 medium resulted in a maximum solvent production of 8.4 g/L at a dilution rate of 0.033 h-1. This resulted in a productivity of 0.27 g/(L h). However, the reactor was not stable and fermentation deteriorated with time. Continuous fermentation of 35 g/L of starch solution resulted in a similar performance. These studies were performed in a vertical column reactor using Clostridium beijerinckii BA101 and P2 medium. It is anticipated that prolonged exposure of culture to acrylamide, which is formed during boiling/autoclaving of starch, affects the fermentation negatively.
University of Illinois, Biotechnology and Bioengineering Group, Department of Food Science and Human Nutrition, 1207 W. Gregory Drive, Urbana, IL 61801, USA.
Thaddeus C
TC
Ezeji
Marisa
M
Groberg
Nasib
N
Qureshi
Hans P
HP
Blaschek
D020001:1-Butanol / Q000378:metabolism*; D019342:Acetic Acid / Q000378:metabolism; D000096:Acetone / Q000378:metabolism; D010367:Arachis / Q000737:chemistry*; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods; D003013:Clostridium / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D012037:Refuse Disposal / Q000379:methods; D012997:Solvents; D013213:Starch
D012997:Solvents; D000096:Acetone; D000431:Ethanol; D020001:1-Butanol; D013213:Starch; D019342:Acetic Acid