Intranuclear organization of RUNX transcriptional regulatory machinery in biological control of skeletogenesis and cancer.
RUNX (AML/CBFA/PEBP2) transcription factors serve as paradigms for obligatory relationships between nuclear structure and physiological control of phenotypic gene expression. The RUNX proteins contribute to tissue restricted transcription by sequence-specific binding to promoter elements of target genes and serving as scaffolds for the assembly of coregulatory complexes that mediate biochemical and architectural control of activity. We will present an overview of approaches we are pursuing to address: (1) the involvement of RUNX proteins in governing competency for protein/DNA and protein/protein interactions at promoter regulatory sequences; (2) the recruitment of RUNX factors to subnuclear sites where the machinery for expression or repression of target genes is organized; and (3) the trafficking and integration of regulatory signals that control RUNX-mediated transcription.
Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA. gary.stein@umassmed.edu
Gary S
GS
Stein
Jane B
JB
Lian
Janet L
JL
Stein
André J
AJ
van Wijnen
Martin
M
Montecino
Jitesh
J
Pratap
Je
J
Choi
S Kaleem
SK
Zaidi
Amjad
A
Javed
Soraya
S
Gutierrez
Kimberly
K
Harrington
Jiali
J
Shen
Daniel
D
Young
D000818:Animals; D001846:Bone Development / Q000235:genetics*; D002843:Chromatin / Q000235:genetics; D050677:Core Binding Factor Alpha 3 Subunit; D050657:Core Binding Factor alpha Subunits; D004268:DNA-Binding Proteins / Q000235:genetics*; D005786:Gene Expression Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics*; D009369:Neoplasms / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics
D002843:Chromatin; D050677:Core Binding Factor Alpha 3 Subunit; D050657:Core Binding Factor alpha Subunits; D004268:DNA-Binding Proteins; D009363:Neoplasm Proteins; C493729:Runx3 protein, human; D014157:Transcription Factors