Day 3 and day 5 morphological predictors of embryo viability.
Controlling multiple pregnancies in patients undergoing artificial reproductive procedures requires consideration of single embryo transfers. Therefore, refinements for embryo evaluation are needed that select for the most developmentally competent embryo. The present study was designed to identify day 3 and day 5 morphological predictors of viability following transfers in which the morphology and fate of each embryo was precisely determined. Assessments on day 3 included cell number, and the extent of fragmentation and asymmetry, and on day 5, the developmental stage. Embryos resulting in a viable fetus at 11 weeks gestation were considered developmentally competent. The relationships among individual and collective embryo morphological characteristics were evaluated. Analysis of the interactions among morphological characteristics of embryos transferred on day 3 enabled identification of a multivariable selection order. Assessment of day 5 embryos revealed that expanding and expanded blastocysts exhibited comparable developmental potential that was superior to that of either morulae or early blastocysts. However, expanding or expanded blastocysts derived from 7-cell or 8-cell embryos were developmentally superior to those derived from other cleavage stages, regardless of fragmentation or asymmetry. Collectively, these findings further understanding of morphological predictors of viability, thereby improving the ability to select the most viable embryo for transfer.
Racowsky
Catherine
C
Department of Obstetrics and Gynecology, Brigham and Women's Hospital, Harvard Medical School, 75 Francis Street, ASB 1+3, Room 082, Boston, MA 02115, USA. cracowsky@partners.org
Combelles
Catherine M H
CM
Nureddin
Aida
A
Pan
Yuying
Y
Finn
Alison
A
Miles
Lyndon
L
Gale
Sarah
S
O'Leary
Thomas
T
Jackson
Katharine V
KV
D000328:Adult; D001755:Blastocyst; D053938:DNA Fragmentation; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000473:pathology / Q000502:physiology*; D005314:Embryonic and Fetal Development; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D005328:Fetal Viability; D006801:Humans; D008423:Maternal Age; D009028:Morula; D009865:Oocytes / Q000378:metabolism; D011247:Pregnancy; D018566:Pregnancy, High-Risk; D013997:Time Factors