Facilitative role of prolactin-releasing peptide neurons in oxytocin cell activation after conditioned-fear stimuli.
Emotional stress activates oxytocin neurons in the hypothalamic supraoptic and paraventricular nuclei and stimulates oxytocin release from the posterior pituitary. Oxytocin neurons in the hypothalamus have synaptic contact with prolactin-releasing peptide (PrRP) neurons. Intracerebroventricular administration of PrRP stimulates oxytocin release from the pituitary. These observations raise the possibility that PrRP neurons play a role in oxytocin response to emotional stress. To test this hypothesis, we first examined expression of Fos protein, an immediate early gene product, in the PrRP neurons in the medulla oblongata after conditioned-fear stimuli. Conditioned-fear stimuli increased the number of PrRP cells expressing Fos protein especially in the dorsomedial medulla. In order to determine whether PrRP cells projecting to the supraoptic nucleus are activated after conditioned-fear stimuli, we injected retrograde tracers into the supraoptic nucleus. Conditioned-fear stimuli induced expression of Fos protein in retrogradely labeled PrRP cells in the dorsomedial medulla. Finally we investigated whether immunoneutralization of endogenous PrRP impairs oxytocin release after emotional stimuli. An i.c.v. injection of a mouse monoclonal anti-PrRP antibody impaired release of oxytocin but not of adrenocorticotrophic hormone or prolactin and did not significantly change freezing behavior in response to conditioned-fear stimuli. From these data, we conclude that PrRP neurons in the dorsomedial medulla that project to the hypothalamus play a facilitative role in oxytocin release after emotional stimuli in rats.
Department of Physiology, Jichi Medical School, Minamikawachi-machi, Tochigi-ken 329-0498, Japan.
L L
LL
Zhu
T
T
Onaka
D000324:Adrenocorticotropic Hormone / Q000378:metabolism; D000818:Animals; D000906:Antibodies / Q000008:administration & dosage; D001522:Behavior, Animal; D002452:Cell Count; D003213:Conditioning, Psychological / Q000502:physiology*; D005239:Fear / Q000502:physiology*; D005456:Fluorescent Dyes / Q000493:pharmacokinetics; D006454:Hemoglobins / Q000378:metabolism; D007028:Hypothalamic Hormones / Q000276:immunology / Q000378:metabolism*; D007032:Hypothalamus, Anterior / Q000033:anatomy & histology / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods; D007276:Injections, Intraventricular / Q000379:methods; D008297:Male; D008526:Medulla Oblongata / Q000033:anatomy & histology / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D009479:Neuropeptides / Q000276:immunology / Q000378:metabolism*; D009994:Osmolar Concentration; D010121:Oxytocin / Q000494:pharmacology*; D056690:Prolactin-Releasing Hormone; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012235:Rhodamines / Q000493:pharmacokinetics
D000906:Antibodies; D005456:Fluorescent Dyes; D006454:Hemoglobins; D007028:Hypothalamic Hormones; D009479:Neuropeptides; C534378:Prlh protein, mouse; C534379:Prlh protein, rat; D056690:Prolactin-Releasing Hormone; D016760:Proto-Oncogene Proteins c-fos; D012235:Rhodamines; D010121:Oxytocin; D000324:Adrenocorticotropic Hormone