[Effect of calcitonin on the proliferation and membrane fluidity of human osteoblast-like cells].
The effects of calcitonin (CT) on the proliferation and membrane fluidity of human osteoblast-like cells line OS-732 were compared with the effect of 1,25(OH)2D3. The rate of DNA synthesis, cell proliferation, cell cycle and membrane fluidity of OS-732 cells were observed. Results showed that 1IU/ml CT stimulated cell proliferation and DNA synthesis. The percentage of cell in the G2 + M phase was increased and the percentage of cell in the synthetic phase was decreased. The membrane fluidity of OS-732 cells was increased after 0.1 mumol/L and 0.01 mumol/L 1,25(OH)2D3 treatment and was decreased after 1IU/ml CT treatment. It is concluded that calcitonin might increase the cell proliferation and decrease the membrane fluidity.
Xue
Y
Y
Beijing Institute of Traumatology and Orthopaedics, Beijing 100035, China.
Yuan
R
R
Xiang
D
D
Wang
H
H
D001859:Bone Neoplasms / Q000473:pathology*; D002116:Calcitonin / Q000494:pharmacology*; D002117:Calcitriol / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D006801:Humans; D008560:Membrane Fluidity / Q000187:drug effects; D018215:Osteoblastoma / Q000473:pathology*; D014407:Tumor Cells, Cultured
D002116:Calcitonin; D002117:Calcitriol