Adsorption of components of enzymatic synthesis of ampicillin on different hydrophobic resins.
This work compared the performance of three hydrophobic resins for the adsorption of ampicillin (AMP), D-phenylglycine (PG), D-phenylglycine methyl ester (PGME), and 6- aminopenicillanic acid (6-APA). The influence of pH on adsorption efficiencies was assessed in the range of 4.5-8.5, at 4 and 25 degrees C. The values at 4 degrees C were slightly higher than those at 25 degrees C. The adsorption efficiency of AMP and 6-APA decreased at higher pHs, for the three resins. An opposite behavior was found for PGME, and the pH did not affect PG adsorption efficiency. Isotherm models were fitted to experimental equilibrium data and the best models were discriminated.
Departamento de Engenharia Química, Universidade Federal de São Carlos, Via Washington Luiz, Km 235, CEP 13565-905, São Carlos, SP, Brazil.
Marcelo F
MF
Vieira
Marlei
M
Barboza
Raquel de Lima C
Rde L
Giordano
D000327:Adsorption; D000667:Ampicillin / Q000302:isolation & purification / Q000378:metabolism*; D002845:Chromatography / Q000379:methods; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010397:Penicillanic Acid / Q000031:analogs & derivatives* / Q000302:isolation & purification; D013816:Thermodynamics
D000667:Ampicillin; D010397:Penicillanic Acid; C004408:aminopenicillanic acid