Comparison of potassium ion preference of potassium-sensing oligonucleotides, PSO-1 and PSO-2, carrying the human and Oxytricha telomeric sequence, respectively.
Human [G(3)(TTAG(3))(3)] and Oxytricha [G(4)(T(4)G(4))(3)] telomere model oligonucleotides, PSO-1 and PSO-2, bearing two fluorophores, 6-carboxyfluorescein (6-FAM) and 6-carboxytetramethylrhodamine (6-TAMRA) at their 5'- and 3'-termini, respectively, were synthesized. Both of them can form an intramolecular antiparallel tetraplex upon addition of K(+), and an enhanced fluorescence resonance energy transfer (FRET) was observed. PSO-1 showed a 43,000 times higher selectivity for K(+) against Na(+). Fluorometric and circular dichroism spectrophotometric studies revealed that this system is useful for the evaluation of the interaction of different telomeric repeat oligonucleotide sequences with metal ions.
Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Fukuoka 812-8581, Japan. staketcm@mbox.nc.kyushu-u.ac.jp
Shigeori
S
Takenaka
Hiroyuki
H
Ueyama
Takahiko
T
Nojima
Makoto
M
Takagi
D000818:Animals; D001483:Base Sequence; D015374:Biosensing Techniques; D002942:Circular Dichroism; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D007477:Ions / Q000378:metabolism; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D016813:Oxytricha / Q000235:genetics*; D011188:Potassium / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism; D016615:Telomere / Q000235:genetics*
D007477:Ions; D009841:Oligonucleotides; D012964:Sodium; D011188:Potassium