Coenzyme Q1-catalyzed luminol chemiluminescent assay for rapid antimicrobial susceptibility testing of Mycobacterium bovis.
Coenzyme Q1 is herein proposed as the best catalyst among coenzymes Q and vitamins K for quinone-catalyzed luminol chemiluminescent assays applied to rapid determination of viability or rapid antimicrobial susceptibility tests of Mycobacterium bovis. Luminol chemiluminescence intensity (LCI) was determined 10 min after the incubation of M. bovis with coenzyme Q1, and was proportional to CFU (colony-forming unit)/ml in the range of 9,000 to 2,250,000. LCI depended on the the production of the superoxide anion (O2-) rather than H2O2 during a 10-min incubation of M. bovis with coenzyme Q1, as superoxide dismutase reduced LCI more effectively than catalase. The minimal inhibitory concentrations (MICs) of 10 kinds of antituberculous agents estimated on the basis of decrease in LCI after one or two days' cultivation were in good agreement with MICs determined by turbidity analysis, which requires upwards of 1 week to complete.
Yamashoji
Shiro
S
Nikken Biomedical Laboratory, 23 Teigaien, Ohashibe, Kumiyama-cho, Kuze-gun, Kyoto 613-0046, Japan. yamashoji@nikken-bio.co.jp
D000904:Antibiotics, Antitubercular / Q000378:metabolism / Q000494:pharmacology*; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry* / Q000378:metabolism; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009163:Mycobacterium bovis / Q000187:drug effects*; D011136:Polysorbates / Q000378:metabolism; D012293:Rifampin / Q000494:pharmacology; D013234:Stem Cells / Q000378:metabolism; D013307:Streptomycin / Q000493:pharmacokinetics / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000378:metabolism; D014451:Ubiquinone / Q000737:chemistry / Q000378:metabolism*; D014812:Vitamin K / Q000378:metabolism
D000904:Antibiotics, Antitubercular; D011136:Polysorbates; C028325:ubiquinone Q3; D014812:Vitamin K; D014451:Ubiquinone; D008165:Luminol; D013482:Superoxide Dismutase; C025203:Ubiquinone Q1; D012293:Rifampin; D013307:Streptomycin