Physiological importance of the connective tissue in the human amnion. Role of magnesium.
The elemental ionic distribution in the epithelial layer (EL) and in connective tissue (CT = compact layer + fibroblast layer) of the human amniotic membrane has been studied in reference samples, after conservation in a physiological fluid (Hanks' solution) and after addition of 2 mM MgCl2 in Hanks' solution. Particle induced X-ray emission and Rutherford backscattering spectrometry techniques were used to provide quantitative measurements. In physiological fluid, with regard to reference samples, the monovalent ions (Na+, K +, Cl-) concentrations were identical on both layers. This data indicates that the connective tissue, in particular the compact layer, acts as a buffer which fix minerals. Mg2+ and Ca2+ levels were higher in EL than in CT. The addition of MgCl2 in Hanks' solution induced a decrease of the monovalent ion concentrations in both layers except Na+ level in EL which remained constant, an increase of the Mg2+ level in both layers, while the Ca2+ remained constant. These data indicate the possible role of connective tissue in pregnancies complicated by poly or oligohydramnios.
Bara
Michel
M
Physiology and Physiopathology Laboratory, University Pierre et Marie Curie, 4, Place Jussieu, 75252 Paris cedex 05, France. Michel.Bara@snv.jussieu.fr
Moretto
Philippe
P
Durlach
Jean
J
Guiet-Bara
Andrée
A
D000650:Amnion / Q000187:drug effects / Q000502:physiology*; D003238:Connective Tissue / Q000187:drug effects / Q000502:physiology*; D004847:Epithelial Cells / Q000187:drug effects / Q000502:physiology*; D005260:Female; D006801:Humans; D008274:Magnesium / Q000502:physiology*; D015636:Magnesium Chloride / Q000494:pharmacology; D011247:Pregnancy
D015636:Magnesium Chloride; D008274:Magnesium