Purification and characterization of an N-acetylglucosamine-binding lectin from Koelreuteria paniculata seeds and its effect on the larval development of Callosobruchus maculatus (Coleoptera: Bruchidae) and Anagasta kuehniella (Lepidoptera: Pyralidae).
This study describes the purification of an N-acetylglucosamine-binding lectin from Koelreuteria paniculata seeds and its effects on the larval development of Callobruchus maculatus and Anagasta kuehniella. The lectin (KpLec) was characterized and isolated by gel filtration, affinity column, and reverse phase chromatography. SDS-PAGE indicated that this lectin is a dimer composed of subunits of 22 and 44 kDa. The N terminus exhibited 40% similarity with Urtiga dioica agglutinin. KpLec was tested for anti-insect activity against C. maculatus and A. kuehniella. With regard to C. maculatus, an artificial diet containing 0.7 and 1% KpLec produced LD(50) and ED(50) value, respectively. However, for A. kuenhiella, an artificial diet containing 0.65% KpLec produced an LD(50), whereas 0.2% KpLec produced an ED(50). The transformation of genes coding for this lectin could be useful in the development of insect resistance in important agricultural crops.
Laboratório de Purificação de Proteínas e suas Funções Biológicas, Departamento de Ciências Naturais, Universidade Federal de Mato Grosso do Sul (UFMS), Avenida Olinto Mancini 1662, Colinos, CP 210, CEP 79603-011 Três Lagoas, MS, Brazil.
Maria Lígia Rodrigues
ML
Macedo
Daniela Carla S
DC
Damico
Maria das Graças Machado
Md
Freire
Marcos H
MH
Toyama
Sérgio
S
Marangoni
José C
JC
Novello
D000117:Acetylglucosamine / Q000378:metabolism*; D000818:Animals; D002845:Chromatography; D001517:Coleoptera / Q000254:growth & development*; D019281:Dimerization; D004591:Electrophoresis, Polyacrylamide Gel; D006384:Hemagglutination; D006801:Humans; D007306:Insecticides; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D007915:Lepidoptera / Q000254:growth & development*; D037121:Plant Lectins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D029631:Sapindaceae / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry
D007306:Insecticides; D037121:Plant Lectins; D000117:Acetylglucosamine