Regulatory effect of IFN-gamma on expression of TGF-beta 1, T beta R-II, and StAR in corpus luteum of pregnant rhesus monkey.
OBJECTIVE
To examine expression of transforming growth factor-beta 1 (TGF-beta 1) and TGF beta receptor II (T beta R-II) and steroidogenic acute regulatory protein (StAR) in the corpus luteum (CL) of pregnant monkeys at various stages and to study possible effect of IFN-gamma on their production.


METHODS
In situ hybridization and immunohistochemistry were applied to detect mRNA and protein.


RESULTS
The expression of StAR, TGF-beta 1, and T beta R-II in the pregnant monkey CL was progressively decreased from d 15 to d 35 of gestation. IFN-gamma down-regulated the expression of TGF-beta 1, T beta R-II, as well as StAR.


CONCLUSIONS
TGF-beta 1 may play an important role in the CL formation and functional maintaining; IFN-gamma down-regulates the expression of TGF-beta 1, T beta R-II, and StAR.
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China.
Xin-Lei
XL
Chen
Hong-Juan
HJ
Gao
Peng
P
Wei
Xin-Xin
XX
Song
Zhao-Yuan
ZY
Hu
Yi-Xun
YX
Liu
D000818:Animals; D003338:Corpus Luteum / Q000187:drug effects* / Q000378:metabolism; D015536:Down-Regulation; D005260:Female; D007371:Interferon-gamma / Q000494:pharmacology*; D008253:Macaca mulatta; D010750:Phosphoproteins / Q000096:biosynthesis* / Q000235:genetics; D011247:Pregnancy; D017346:Protein Serine-Threonine Kinases; D012333:RNA, Messenger / Q000096:biosynthesis / Q000187:drug effects; D000077294:Receptor, Transforming Growth Factor-beta Type II; D018125:Receptors, Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics; D053773:Transforming Growth Factor beta1; D000098682:Steroidogenic Acute Regulatory Protein
D010750:Phosphoproteins; D012333:RNA, Messenger; D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D000098682:Steroidogenic Acute Regulatory Protein; D007371:Interferon-gamma; D017346:Protein Serine-Threonine Kinases; D000077294:Receptor, Transforming Growth Factor-beta Type II