Evidence for a role of MCM (mini-chromosome maintenance)5 in transcriptional repression of sub-telomeric and Ty-proximal genes in Saccharomyces cerevisiae.
The MCM (mini-chromosome maintenance) genes have a well established role in the initiation of DNA replication and in the elongation of replication forks in Saccharomyces cerevisiae. In this study we demonstrate elevated expression of sub-telomeric and Ty retrotransposon-proximal genes in two mcm5 strains. This pattern of up-regulated genes resembles the genome-wide association of MCM proteins to chromatin that was reported earlier. We link the altered gene expression in mcm5 strains to a reversal of telomere position effect (TPE) and to remodeling of sub-telomeric and Ty chromatin. We also show a suppression of the Ts phenotype of a mcm5 strain by the high copy expression of the TRA1 component of the chromatin-remodeling SAGA/ADA (SPT-ADA-GCN5 acetylase/ADAptor). We propose that MCM proteins mediate the establishment of silent chromatin domains around telomeres and Ty retrotransposons.
Dziak
Renata
R
Department of Molecular Biology and Genetics, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
Leishman
David
D
Radovic
Maja
M
Tye
Bik K
BK
Yankulov
Krassimir
K
D015152:Blotting, Northern; D018797:Cell Cycle Proteins / Q000502:physiology*; D002843:Chromatin / Q000378:metabolism; D004268:DNA-Binding Proteins; D015966:Gene Expression Regulation, Fungal; D016681:Genome, Fungal; D007700:Kinetics; D008836:Micrococcal Nuclease / Q000378:metabolism; D008957:Models, Genetic; D020411:Oligonucleotide Array Sequence Analysis; D016366:Open Reading Frames; D010641:Phenotype; D018626:Retroelements; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins; D016615:Telomere / Q000648:ultrastructure*; D013696:Temperature; D013997:Time Factors; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D015854:Up-Regulation
D018797:Cell Cycle Proteins; D002843:Chromatin; D004268:DNA-Binding Proteins; D018626:Retroelements; D029702:Schizosaccharomyces pombe Proteins; C085711:mcm5 protein, S pombe; D008836:Micrococcal Nuclease