Alkaline degradation kinetics and CE-separation of cello- and xylooligomers. Part I.
The degradation kinetics of cello- and xylooligomers under alkaline conditions has been studied, and kinetic order, kinetic rate constants as well as activation parameters (E(A), DeltaE(#), DeltaS(#)) for the different oligomers have been determined. The results were corroborated by a mathematical model of the degradation kinetics. A reliable and convenient method for the separation and simultaneous quantification of cello- and xylooligomers, based on capillary electrophoresis (CE) with pre-column derivatization, has been established. p-Aminobenzonitrile was used as the UV tag, and 550 mM borate buffer containing hexadimethrine bromide was employed as the running electrolyte.
Christian-Doppler-Laboratory, Institute of Chemistry, University of Agricultural Sciences, Muthgasse 18, A-1190, Vienna, Austria.
Jürgen
J
Sartori
Antje
A
Potthast
Alois
A
Ecker
Herbert
H
Sixta
Thomas
T
Rosenau
Paul
P
Kosma
D000468:Alkalies / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008956:Models, Chemical; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
D000468:Alkalies; D009844:Oligosaccharides