Genetic organization of genes encoding phenol hydroxylase, benzoate 1,2-dioxygenase alpha subunit and its regulatory proteins in Acinetobacter calcoaceticus PHEA-2.
Acinetobacter calcoaceticus PHEA-2 is a phenol-degrading bacterium isolated from the wastewater from an oil refinery. A 10-kb XhoI fragment consisting of nine complete Open Reading Frames (ORFs) and one partial ORF was screened from a lambda library of PHEA-2 by Southern hybridization. The sequence analyses revealed that ORF2-ORF7, designated mphKLMNOP, are homologous to dmpKLMNOP of Pseudomonas sp. CF600 and mopKLMNOP of Acinetobacter calcoaceticus NCIB8250, sharing 38%-72% and 58.5%-93.5% respectively. The products encoded by dmp and mop genes convert phenol to catechol. The mph-operon and downstream ORFs, ORF9 and ORF10, sharing high identities to benM and benA, which encode ben-operon regulatory protein and benzoate 1,2-dioxygenase alpha subunit respectively, are separated by ORF8, whose function is unknown. The organization of the mph and ben operons is different from that described previously.
Department of Biotechnology, Institute for Application of Atomic Energy, Chinese Academy of Agricultural Sciences, P. O. Box 5109, Beijing, 100094, China.
Yuquan
Y
Xu
Ming
M
Chen
Wei
W
Zhang
Min
M
Lin
D016954:Acinetobacter calcoaceticus / Q000201:enzymology* / Q000235:genetics*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D040461:Gene Components; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D005798:Genes, Bacterial; D006824:Hybridization, Genetic; D006899:Mixed Function Oxygenases / Q000235:genetics*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010105:Oxygenases / Q000235:genetics*; D016415:Sequence Alignment
D001426:Bacterial Proteins; D006899:Mixed Function Oxygenases; D010105:Oxygenases; C019766:benzoate 1,2-dioxygenase; C022336:phenol 2-monooxygenase