Anti-Candida antibodies in serum and saliva of patients with denture stomatitis.
OBJECTIVE
Quantitative measurement of IgA and IgG antibodies in the serum and saliva of patients with denture stomatitis (stomatitis subprothetica) was the object of this study.


METHODS
The indirect immunofluorescence technique (IFA) utilizing CIP 628 (serotype A) Candida albicans blastospores was employed. Anti-human goat and rabbit fluorescein isothiocyanate labeled serum was used. Serial serum dilutions from 1:20 to 1:1280 and saliva serial dilutions from 1:1 to 1:8 were prepared. Specimens were read using an ML-2 fluorescence microscope. Alternative analysis, analysis of variance and graphical analysis was used for statistical analysis of data.


RESULTS
Anti-Candida antibodies (mainly IgG class) were detectable at dilutions of 1:40, 1:80 and 1:160 in the serum of patients with denture stomatitis compared with healthy controls. The saliva samples of these patients showed higher titers of anti-Candida antibodies compared with controls (1:8 for IgG and 1:4 for IgA).


CONCLUSIONS
IgG anti-bodies were prevalent in the saliva of our patients and they were of diagnostic value. The IgA titers did not remain constant and could be a determinant of the disease course. Each patient showed an individual serologic profile. For this reason individualized interpretation of saliva antibodies as well as a comparison with the serum anti-Candida antibodies is recommended.
National Reference Laboratory of Mycology, Department of Microbiology, National Center of Infectious and Parasitic Diseases, Sofia, Bulgaria.
Todor V
TV
Kantardjiev
Elka V
EV
Popova
D000908:Antibodies, Fungal / Q000032:analysis / Q000097:blood*; D002175:Candida / Q000276:immunology*; D002180:Candidiasis, Oral / Q000175:diagnosis*; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis / Q000097:blood; D007074:Immunoglobulin G / Q000032:analysis / Q000097:blood; D012463:Saliva / Q000276:immunology*; D013282:Stomatitis, Denture / Q000276:immunology* / Q000382:microbiology*
D000908:Antibodies, Fungal; D007070:Immunoglobulin A; D007074:Immunoglobulin G