The Genome sequence of the SARS-associated coronavirus.
We sequenced the 29,751-base genome of the severe acute respiratory syndrome (SARS)-associated coronavirus known as the Tor2 isolate. The genome sequence reveals that this coronavirus is only moderately related to other known coronaviruses, including two human coronaviruses, HCoV-OC43 and HCoV-229E. Phylogenetic analysis of the predicted viral proteins indicates that the virus does not closely resemble any of the three previously known groups of coronaviruses. The genome sequence will aid in the diagnosis of SARS virus infection in humans and potential animal hosts (using polymerase chain reaction and immunological tests), in the development of antivirals (including neutralizing antibodies), and in the identification of putative epitopes for vaccine development.
Marra
Marco A
MA
British Columbia Cancer Agency (BCCA) Genome Sciences Centre, 600 West 10th Avenue, Vancouver, British Columbia V5Z 4E6, Canada. mmarra@bccgsc.ca
Jones
Steven J M
SJ
Astell
Caroline R
CR
Holt
Robert A
RA
Brooks-Wilson
Angela
A
Butterfield
Yaron S N
YS
Khattra
Jaswinder
J
Asano
Jennifer K
JK
Barber
Sarah A
SA
Chan
Susanna Y
SY
Cloutier
Alison
A
Coughlin
Shaun M
SM
Freeman
Doug
D
Girn
Noreen
N
Griffith
Obi L
OL
Leach
Stephen R
SR
Mayo
Michael
M
McDonald
Helen
H
Montgomery
Stephen B
SB
Pandoh
Pawan K
PK
Petrescu
Anca S
AS
Robertson
A Gordon
AG
Schein
Jacqueline E
JE
Siddiqui
Asim
A
Smailus
Duane E
DE
Stott
Jeff M
JM
Yang
George S
GS
Plummer
Francis
F
Andonov
Anton
A
Artsob
Harvey
H
Bastien
Nathalie
N
Bernard
Kathy
K
Booth
Timothy F
TF
Bowness
Donnie
D
Czub
Martin
M
Drebot
Michael
M
Fernando
Lisa
L
Flick
Ramon
R
Garbutt
Michael
M
Gray
Michael
M
Grolla
Allen
A
Jones
Steven
S
Feldmann
Heinz
H
Meyers
Adrienne
A
Kabani
Amin
A
Li
Yan
Y
Normand
Susan
S
Stroher
Ute
U
Tipples
Graham A
GA
Tyler
Shaun
S
Vogrig
Robert
R
Ward
Diane
D
Watson
Brynn
B
Brunham
Robert C
RC
Krajden
Mel
M
Petric
Martin
M
Skowronski
Danuta M
DM
Upton
Chris
C
Roper
Rachel L
RL
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D017934:Coronavirus / Q000145:classification / Q000235:genetics; D000086862:Coronavirus M Proteins; D000086462:Coronavirus Nucleocapsid Proteins; D018076:DNA, Complementary; D018965:Frameshifting, Ribosomal; D016679:Genome, Viral; D006801:Humans; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics; D019590:Nucleocapsid Proteins / Q000737:chemistry / Q000235:genetics; D016366:Open Reading Frames; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D012324:RNA-Dependent RNA Polymerase / Q000737:chemistry / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D045473:Severe acute respiratory syndrome-related coronavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D045169:Severe Acute Respiratory Syndrome / Q000821:virology; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics; D014763:Viral Matrix Proteins / Q000737:chemistry / Q000235:genetics; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000086862:Coronavirus M Proteins; D000086462:Coronavirus Nucleocapsid Proteins; D018076:DNA, Complementary; C000706232:M protein, Human coronavirus OC43; C000706237:M protein, SARS-CoV; D008562:Membrane Glycoproteins; C000706247:N protein, SARS-CoV; D019590:Nucleocapsid Proteins; D012367:RNA, Viral; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins; D014763:Viral Matrix Proteins; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase