Collagenases (MMP-1, -8 and -13) and trypsinogen-2 in fluid from benign and malignant ovarian cysts.
Proteolytic enzymes, such as matrix metalloproteinases (MMPs) and tumor-associated trypsinogens (TAT), play a pivotal role in tumor invasion and metastasis. Among MMPs, the interstitial collagenases (MMP-1, -8 and -13) can initiate collagenolysis. In this study, we have studied the levels of MMP-1, -8 and -13 in relation to the level of trypsinogen-2 in fluid from benign and malignant ovarian cysts. Elevated MMP-8 levels occur in many ovarian cyst fluids, and high MMP-8 levels are associated with malignancy. The concentrations of trypsinogen-2 correlate with those of MMP-8, but it remains to be shown whether trypsin-2 plays a role as its activator in vivo. The strong expression of MMP-8 over MMP-1 and MMP-13 in malignant ovarian tumors may indicate that MMP-8 participates in the protease cascades associated with the invasiveness of ovarian tumors.
Stenman
M
M
Department of Internal Medicine, Orton Research Institute, Helsinki, Finland. mathias.stenman@helsinki.fi
Paju
A
A
Hanemaaijer
R
R
Tervahartiala
T
T
Leminen
A
A
Stenman
U-H
UH
Konttinen
Y T
YT
Sorsa
T
T
D000293:Adolescent; D000328:Adult; D000368:Aged; D017364:Collagenases / Q000032:analysis*; D005260:Female; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000032:analysis; D053509:Matrix Metalloproteinase 13; D020784:Matrix Metalloproteinase 8 / Q000032:analysis; D008875:Middle Aged; D010048:Ovarian Cysts / Q000737:chemistry*; D014357:Trypsin; D014362:Trypsinogen / Q000032:analysis
C064831:PRSS2 protein, human; D014362:Trypsinogen; D014357:Trypsin; D017364:Collagenases; C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13; D020784:Matrix Metalloproteinase 8; D020781:Matrix Metalloproteinase 1