Amperometric detection of perphenazine at a carbon fiber micro-disk bundle electrode by capillary zone electrophoresis.
A simple method for determination of perphenazine by capillary zone electrophoresis with amperometric detection is described. The optimum conditions of separation and detection are 1.50 x 10(-3) mol/l Na(2)B(4)O(7)-1.0 x 10(-3) mol/l NaOH (pH 9.9) for the buffer solution, 18 kV for the separation voltage, 5 kV and 5 s for the injection voltage and the injection time, and 0.80 V versus saturated calomel electrode for the detection potential, respectively. The limit of detection is 5.0 x 10(-8) mol/l or 44 amol (S/N=3). The linear range of the calibration curve is 1.00 x 10(-7) to 1.00 x 10(-4) mol/l. The relative standard deviation is 1.5% for the migration time and 2.9% for the electrophoretic current at peak maximum. The method is applied to the determination of perphenazine in human urine.
Liu
Daxing
D
School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China.
Jin
Wenrui
W
D014150:Antipsychotic Agents / Q000652:urine*; D002138:Calibration; D002244:Carbon / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D010546:Perphenazine / Q000652:urine*
D014150:Antipsychotic Agents; D002244:Carbon; D010546:Perphenazine