Peptone-liver (P-L) a new culture medium for the Egyptian Leishmania major.
Leishmania major promastigotes isolated from an Egyptian ZCL patient, was inoculated 4 x 10(6)/ml in a new culture medium, peptone-liver (P-L), with a final concentration of 10% of FCS (fetal calf serum), and incubated at 25+/-1 degrees C. Meanwhile, the same number of promastigotes were inoculated in Schneider's Drosophila medium as control. On the 10th day, number of L. major promastigotes reached 29,25 x 10(6)/ml. The same number was obtained with Schneider's Drosophila medium within four days. The new culture medium or P-L medium is cheap, easily prepared, and gave a very good number of promastigotes as in Schneider's Drosophila medium but in a longer time.
Ain Shams Research and Training Center on Vectors of Diseases, Ain Shams University, Cairo 11566, Egypt.
Said A M
SA
Mazyad
Ahmed
A
Abdel-Aziz
D000818:Animals; D003470:Culture Media / Q000737:chemistry* / Q000191:economics; D004534:Egypt; D018320:Leishmania major / Q000502:physiology*; D008099:Liver; D010461:Peptones
D003470:Culture Media; D010461:Peptones