D-Xylose transport by Candida succiphila and Kluyveromyces marxianus.
The kinetics and regulation of D-xylose uptake were investigated in the efficient pentose fermentor Candida succiphila, and in Kluyveromyces marxianus, which assimilate but do not ferment pentose sugars. Active high affinity (Km approximately 3.8 mM; Vmax approximately 15 nmol/[mg min]) and putative facilitated diffusion low-affinity (Km approximately 140 mM; Vmax approximately 130 nmol/[mg min]) transport activities were found in C. succiphila grown, respectively, on xylose or glucose. K. marxianus showed facilitated diffusion low-affinity (Km approximately 103 mM; Vmax approximately 190 nmol/[mg min]) transport activity when grown on xylose under microaerobic conditions, and both a low-affinity and an active high-affinity (Km approximately 0.2 mM; Vmax approximately 10 nmol/[mg min]) transport activity when grown on xylose under fully aerobic conditions.
National Bioenergy Center, National Renewable Energy Laboratory, 1617 Cole Boulevard, Golden, CO 80401, USA. bstambuk@mbox1.ufsc.br
Boris U
BU
Stambuk
Mary Ann
MA
Franden
Arjun
A
Singh
Min
M
Zhang
D000332:Aerobiosis; D001692:Biological Transport / Q000187:drug effects; D002175:Candida / Q000254:growth & development / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D007700:Kinetics; D007716:Kluyveromyces / Q000254:growth & development / Q000378:metabolism*; D009005:Monosaccharides / Q000494:pharmacology; D014994:Xylose / Q000378:metabolism*
D009005:Monosaccharides; D014994:Xylose; D005947:Glucose