One-step immunochromatographic dipstick tests for rapid detection of Vibrio cholerae O1 and O139 in stool samples.
We describe the development and evaluation of a rapid diagnostic test for Vibrio cholerae O1 and O139 based on lipopolysaccharide detection using gold particles. The specificity ranged between 84 and 100%. The sensitivity of the dipsticks ranged from 94.2 to 100% when evaluated with stool samples obtained in Madagascar and Bangladesh. The dipstick can provide a simple tool for epidemiological surveys.
Institut Pasteur, Paris, France. fnato@pasteur.fr
F
F
Nato
A
A
Boutonnier
M
M
Rajerison
P
P
Grosjean
S
S
Dartevelle
A
A
Guénolé
N A
NA
Bhuiyan
D A
DA
Sack
G B
GB
Nair
J M
JM
Fournier
S
S
Chanteau
D001459:Bangladesh; D002771:Cholera / Q000175:diagnosis*; D002846:Chromatography, Affinity / Q000379:methods; D004768:Enterotoxins / Q000032:analysis*; D005243:Feces / Q000382:microbiology*; D017979:Gold Colloid; D006801:Humans; D008270:Madagascar; D012680:Sensitivity and Specificity
D004768:Enterotoxins; D017979:Gold Colloid; C037420:stN protein, Vibrio cholerae