[Biomarkers in the studies on chemoprevention of colorectal cancer].
The present study was to investigate the chemopreventive effects of tea on colorectal cancer with a series of biomarkers. The results showed that the number of silver-stained nucleolar organizer dots per nucleus(AgNORs), labeling index(LI) of proliferating cell nuclear antigen(PCNA) of intestinal mucosa, and the expression of ras-p21 protein were significantly reduced in the tea-treated groups(P < 0.01) as compared with the positive control group. Furthermore, tea and tea pigments inhibited the expression of Bcl-2 protein and induced the expression of Bax protein(P < 0.05 or P < 0.01). The study provided evidence supporting that PCNA-LI, AgNORs, Bcl-2, Bax and ras-p21 protein could be used as effective biomarkers for colorectal carcinogenesis in human chemopreventive trials.
Institute of Nutrition and Food Hygiene, Chinese Academy of Preventive Medicine, Beijing 100050, China.
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Jia
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Han
D000818:Animals; D000831:Animals, Newborn; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D014408:Biomarkers, Tumor / Q000032:analysis*; D015179:Colorectal Neoplasms / Q000737:chemistry* / Q000517:prevention & control; D005419:Flavonoids; D008297:Male; D010636:Phenols / Q000494:pharmacology*; D010860:Pigments, Biological / Q000494:pharmacology; D011108:Polymers / Q000494:pharmacology*; D059808:Polyphenols; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D016283:Proto-Oncogene Proteins p21(ras) / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D013662:Tea / Q000737:chemistry*
D000972:Antineoplastic Agents, Phytogenic; D014408:Biomarkers, Tumor; D005419:Flavonoids; D010636:Phenols; D010860:Pigments, Biological; D011108:Polymers; D059808:Polyphenols; D018809:Proliferating Cell Nuclear Antigen; D019253:Proto-Oncogene Proteins c-bcl-2; D013662:Tea; D016283:Proto-Oncogene Proteins p21(ras)