Detection and quantification of 5-hydroxyoxindole in mammalian sera and tissues by high performance liquid chromatography with multi-electrode electrochemical detection.
OBJECTIVE
Since 5-hydroxyoxindole structurally related indole metabolites play different roles in some hepatic and neurologic disorders we found necessary to develop an assay to further investigate the physiologic relevance of this compound.


METHODS
We have designed a convenient assay to determine 5-hydroxyoxindole in serum using solid phase extraction and a highly selective High Performance Liquid Chromatography system with multi-Electro Chemical Detection (HPLC-ECD).


RESULTS
We have identified and quantified 5-hydroxyoxindole in various mammalian species. Its distribution in tissues showed that the molecule is also present in brain, liver, kidney and spleen, but not in skeletal muscle.


CONCLUSIONS
5-hydroxyoxindole is an endogenous tryptophan metabolite present in circulating blood and in some tissues at the nmol level, its determination using HPLC-ECD will be useful for elucidating the role of this molecule in normal and disease conditions.
Valbiofrance, 151 Boulevard du Montparnasse, 75006 Paris, France.
Dulce
D
Papy-García
V éronique
Vé
Barbier V
Marie-Claude
MC
Tournaire
Agnés
A
Cane
Henry
H
Brugère
Michèle
M
Crumeyrolle-Arias
Denis
D
Barritault
D000818:Animals; D001923:Brain Chemistry; D002138:Calibration; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D006801:Humans; D007211:Indoles / Q000032:analysis* / Q000097:blood* / Q000592:standards; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008956:Models, Chemical; D012756:Sheep; D013154:Spleen / Q000737:chemistry
D007211:Indoles; C026785:5-hydroxyindole