Promoter of the rolC gene of Agrobacterium rhizogenes can be strongly regulated in glandular cell of transgenic tobacco.
A 577-bp promoter segment of Agrobacterium rhizogenes rolC, previously known as the phloem-specific gene expression promoter, was fused to the 5' end of a reporter gene, beta-glucuronidase (GUS), uidA. This rolC-promoter-driven expression of the GUS gene was found to be significantly strong in glandular cells in transgenic tobacco plants. Analysis of this segment of the promoter sequence revealed a myb response element.
Life Sciences Center, College of Life Science, Peking University, Beijing 100871, China.
Yuanlei
Y
Hu
Bojun
B
Chen
Ting
T
Ni
Ning
N
Li
Zhongping
Z
Lin
D001483:Base Sequence; D004789:Enzyme Activation; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics; D018517:Plant Roots / Q000201:enzymology / Q000235:genetics; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012231:Rhizobium / Q000201:enzymology* / Q000235:genetics*; D014026:Nicotiana / Q000201:enzymology* / Q000235:genetics*; D001617:beta-Glucosidase / Q000235:genetics* / Q000378:metabolism*
D011993:Recombinant Fusion Proteins; C071257:cytokinin-beta-glucosidase; D001617:beta-Glucosidase; D005966:Glucuronidase