Treatment of PBMC with killed Helicobacter pylori subverts the environment of inflammatory cytokines.
It is well known that inflammation induced by Helicobacter pylori is characterized by the local production of cytokines and chemokines. In the present study, we analyse the kinetics of MCP-1, IL-12 and IL-4 induction during the interaction of peripheral blood mononuclear cells with killed and/or live H. pylori. Our results demonstrate that live H. pylori does not induce IL-4 release whereas it stimulates MCP-1 and IL-12 production. In addition, the neutralization of IL-12 with monoclonal antibodies determines a lower MCP-1 release. These data demonstrate that MCP-1 production is in part supported by IL-12 induced by live H. pylori. On the contrary, killed H. pylori stimulates the IL-4 but not MCP-1 and IL-12 production. The combined treatment with killed and live H. pylori upregulates the IL-4 production and at the same time downregulates IL-12 and MCP-1 production.
Department of Surgical Sciences, Policlinico G. MARTINO, University of Messina, Italy.
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G
Stassi
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Arena
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Speranza
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Iannello
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M
Miceli
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P
Mastroeni
D018932:Chemokine CCL2 / Q000032:analysis / Q000096:biosynthesis; D016207:Cytokines / Q000096:biosynthesis*; D004306:Dose-Response Relationship, Immunologic; D016480:Helicobacter pylori / Q000737:chemistry / Q000472:pathogenicity*; D006801:Humans; D007249:Inflammation / Q000276:immunology; D018664:Interleukin-12 / Q000032:analysis / Q000096:biosynthesis; D015847:Interleukin-4 / Q000032:analysis / Q000096:biosynthesis; D007700:Kinetics; D007963:Leukocytes, Mononuclear / Q000276:immunology*
D018932:Chemokine CCL2; D016207:Cytokines; D018664:Interleukin-12; D015847:Interleukin-4