Conservation and molecular dissection of ROUGH SHEATH2 and ASYMMETRIC LEAVES1 function in leaf development.
Maize ROUGH SHEATH2 (RS2) and Arabidopsis ASYMMETRIC LEAVES1 (AS1) are orthologous Myb-related genes required for leaf development and act as negative regulators of class 1 KNOTTED1-like homeobox (KNOX) genes in leaf primordia. Expression of RS2 in Arabidopsis fully complements as1 leaf phenotypes and represses the expression of the KNOX gene KNAT1 in leaves. Whereas loss of AS1 function in Arabidopsis results in rounded, lobed leaves with shorter and wider petioles, overexpression of either RS2 or AS1 results in longer and narrower leaves with longer petioles than wild type. A conserved C-terminal domain (CTD) mediates homodimerization of both RS2 and AS1 and modulates leaf shape when expressed independently of the Myb domain in Arabidopsis. Homodimerization is not absolutely required for KNAT1 repression. RS2:GFP fusion protein is biologically active, localized in discrete dynamic subnuclear foci and associates with DNA during cell division.
Theodoris
George
G
Department of Plant and Microbial Biology, University of California, Berkeley 94720, USA.
Inada
Noriko
N
Freeling
Michael
M
D017360:Arabidopsis; D029681:Arabidopsis Proteins / Q000502:physiology*; D001483:Base Sequence; D017931:DNA Primers; D019281:Dimerization; D018515:Plant Leaves / Q000254:growth & development*; D010940:Plant Proteins; D030821:Plants, Genetically Modified; D012097:Repressor Proteins / Q000502:physiology*; D014157:Transcription Factors
C456239:ASYMMETRIC LEAVES1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D017931:DNA Primers; D010940:Plant Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; C118404:rough sheath2 protein, Zea mays