Determination of huperzine A in formulated products by reversed-phase-liquid chromatography using diode array and electrospray ionization mass spectrometric detection.
A precise and selective high-performance liquid chromatographic (HPLC) method with diode-array detection for quantifying huperzine A in formulated products was developed and validated. A liquid chromatographic-mass spectrometric (LC/MS) procedure was devised to confirm the HPLC method. Huperzine A was dissolved in 1,2-dichloroethane, chromatographed on a YMCBasic C18 column, and detected at 308 nm. A gradient mobile phase of 10 mM ammonium acetate (pH = 3.5)--methanol was used. Identification was based on retention time, UV spectra and mass spectra by comparison with a commercial standard. The UV peak areas were used for quantitation of huperzine A content. The correlation coefficient (R2) of the calibration curve was 1 over the range 0.8-11.6 microg/ml. Overall recovery of huperzine A was 103.9% +/- 1.8 (mean +/- SD). Relative standard deviations for intra- and interday precision were < 2%.
Yang
Q P
QP
Analytical Methods Development Group, Research and Development Department, Nature's Sunshine Products, Spanish Fork, UT 84660, USA.
Kou
X L
XL
Fugal
K B
KB
McLaughlin
J L
JL
D000470:Alkaloids; D001688:Biological Products / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D032501:Huperzia; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D012717:Sesquiterpenes / Q000737:chemistry* / Q000302:isolation & purification; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013057:Spectrum Analysis
D000470:Alkaloids; D001688:Biological Products; D010936:Plant Extracts; D012717:Sesquiterpenes; C050426:huperzine A