Changes of pulmonary intercellular adhesion molecule-1 and CD11b/CD18 in peripheral polymorphonuclear neutrophils and their significance at the early stage of burns.
OBJECTIVE
To investigate the role of intercellular adhesion molecule-1 (ICAM-1) in the accumulation of polymorphonuclear neutrophils (PMN) in the lungs at the early stage of burns.


METHODS
Myeloperoxidase content in lung tissues and bronchoalveolar lavage fluid (BALF) were detected. ICAM-1 and its mRNA expression in lung tissues were determined by immunohistochemical method and in situ hybridization. CD11b/CD18 expression on the peripheral PMNs was measured by flow cytometry.


RESULTS
The levels of myeloperoxidase in lung tissues and BALF after burn injury were markedly higher than those of control. Expression of ICAM-1 and its mRNA in the lung tissues and CD11b/CD18 on peripheral PMNs surface was significantly increased at 2, 6, 12, 24 h after burns.


CONCLUSIONS
PMNs accumulation in the lungs is related to increased ICAM-1 expression on pulmonary microvascular endothelial cells and CD11b/CD18 expression on PMN at the early stage of burn injury.
Research Institute of Surgery, Third Military Medical University, Chongqing 400042, China. jinrbz@cta.cq.cn
Rong-bing
RB
Jin
Pei-fang
PF
Zhu
Zheng-guo
ZG
Wang
Da-wei
DW
Liu
Ji-hong
JH
Zhou
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002056:Burns / Q000097:blood / Q000276:immunology*; D002448:Cell Adhesion; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis*; D008168:Lung / Q000098:blood supply; D016177:Macrophage-1 Antigen / Q000032:analysis*; D009504:Neutrophils / Q000276:immunology* / Q000473:pathology; D009195:Peroxidase / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D013997:Time Factors
D016177:Macrophage-1 Antigen; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1; D009195:Peroxidase