AT1 receptor blockade improves vasorelaxation in experimental renal failure.
It is not known whether angiotensin II type 1 receptor antagonists can influence the function and morphology of small arteries in renal failure. We investigated the effect of 8-week losartan therapy (20 mg/kg per day) on isolated mesenteric resistance arteries by wire and pressure myographs in 5/6 nephrectomized rats. Plasma urea nitrogen was elevated 1.6-fold after nephrectomy, and ventricular synthesis of atrial and B-type natriuretic peptides was increased 2.2-fold and 1.7-fold, respectively, whereas blood pressure was not affected. Losartan did not influence these variables. The endothelium-mediated relaxation to acetylcholine was impaired in nephrectomized rats in the absence and presence of nitric oxide synthase and cyclooxygenase inhibition. Blockade of calcium-activated potassium channels by charybdotoxin and apamin reduced the remaining acetylcholine response, and this effect was less marked in nephrectomized than in sham-operated rats. Relaxation to levcromakalim, a vasodilator acting through adenosine triphosphate-sensitive potassium channels, was also impaired after nephrectomy. The arteries of nephrectomized rats showed eutrophic inward remodeling: Wall-to-lumen ratio was increased without change in wall cross-sectional area. All changes in arterial relaxation and morphology were normalized by losartan therapy. Aortic ACE content, measured by autoradiography, directly correlated to the plasma level of urea nitrogen, suggesting that renal failure has an enhancing influence on the vascular renin-angiotensin system. Losartan normalized relaxation and morphology of resistance arteries in experimental renal failure, independent of its influence on blood pressure, impaired kidney function, or volume overload. The mechanism of improved vasodilation by losartan may include enhanced relaxation through potassium channels.
Department of Pharmacological Sciences, University of Tampere, Finland.
Peeter
P
Kööbi
Jarkko
J
Kalliovalkama
Pasi
P
Jolma
Jaana
J
Rysä
Heikki
H
Ruskoaho
Olli
O
Vuolteenaho
Mika
M
Kähönen
Ilkka
I
Tikkanen
Meng
M
Fan
Pauli
P
Ylitalo
Ilkka
I
Pörsti
D057911:Angiotensin Receptor Antagonists; D000818:Animals; D001011:Aorta / Q000737:chemistry; D009320:Atrial Natriuretic Factor / Q000096:biosynthesis / Q000097:blood / Q000235:genetics; D001794:Blood Pressure; D004730:Endothelium, Vascular / Q000502:physiology; D006352:Heart Ventricles / Q000378:metabolism; D066298:In Vitro Techniques; D019808:Losartan / Q000627:therapeutic use; D008297:Male; D008638:Mesenteric Arteries / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D020097:Natriuretic Peptide, Brain / Q000096:biosynthesis / Q000235:genetics; D009392:Nephrectomy; D007703:Peptidyl-Dipeptidase A / Q000032:analysis; D026902:Potassium Channel Blockers / Q000494:pharmacology; D024681:Potassium Channels, Calcium-Activated / Q000037:antagonists & inhibitors; D011498:Protein Precursors / Q000097:blood; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D044140:Receptor, Angiotensin, Type 1; D051437:Renal Insufficiency / Q000188:drug therapy / Q000473:pathology / Q000503:physiopathology*; D014661:Vasoconstriction; D014664:Vasodilation / Q000187:drug effects*
D057911:Angiotensin Receptor Antagonists; C099254:N-terminal proatrial natriuretic peptide; D026902:Potassium Channel Blockers; D024681:Potassium Channels, Calcium-Activated; D011498:Protein Precursors; D012333:RNA, Messenger; D044140:Receptor, Angiotensin, Type 1; D020097:Natriuretic Peptide, Brain; D009320:Atrial Natriuretic Factor; D007703:Peptidyl-Dipeptidase A; D019808:Losartan