Effective chemical preservation of morphology of urinary erythrocytes.
Between 2 and 4 h after miction the morphology of urinary erythrocytes has changed, clouding the distinction between glomerular and non-glomerular bleeding in pediatric patients. Glomerular bleeding is characterized by microscopically visible alteration in urinary erythrocytes due to glomerular disease. Fixation by Cellfix, a formaldehyde-based fixative, allows the preservation of the morphology for at least 24 h and can be recommended for clinical practice. In our experience, thiomersal was not effective for preservation of the morphology of erythrocytes.
Komarova
Olga
O
Department of Pediatric Nephrology, Institute of Pediatrics, RAMN, Moscow, Russia.
van der Meer
Wim
W
Levtchenko
Elena
E
Monnens
Leo
L
D000050:Acanthocytes / Q000648:ultrastructure; D002648:Child; D004912:Erythrocytes / Q000648:ultrastructure*; D005404:Fixatives; D005557:Formaldehyde; D005921:Glomerulonephritis / Q000097:blood / Q000652:urine; D005922:Glomerulonephritis, IGA / Q000097:blood / Q000652:urine; D015432:Glomerulonephritis, Membranoproliferative / Q000097:blood / Q000652:urine; D006417:Hematuria / Q000097:blood*; D006801:Humans; D008858:Microscopy, Phase-Contrast; D013048:Specimen Handling / Q000379:methods*; D013997:Time Factors; D016707:Tissue Fixation / Q000379:methods*
D005404:Fixatives; D005557:Formaldehyde