Mutations in the rpoB gene of multidrug-resistant Mycobacterium tuberculosis isolates from China.
Mutations in the 81-bp rifampin resistance determining region (RRDR) and mutation V176F locating at the beginning of the ropB gene were analyzed by DNA sequencing of 86 Mycobacterium tuberculosis clinical isolates (72 resistant and 14 sensitive) from different parts of China. Sixty-five mutations of 22 distinct kinds, 21 point mutations, and 1 insertion were found in 65 of 72 resistant isolates. The most common mutations were in codons 531 (41%), 526 (40%), and 516 (4%). Mutations were not found in seven (10%) of the resistant isolates. Six new alleles within the RRDR, along with five novel mutations outside the RRDR, are reported. None of isolates contained the V176 mutation.
Yue
Jun
J
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University and Shanghai Pneumology Hospital, Shanghai 200433, People's Republic of China.
Shi
Wei
W
Xie
Jingping
J
Li
Yao
Y
Zeng
Erliang
E
Wang
Honghai
H
D000483:Alleles; D000595:Amino Acid Sequence; D000904:Antibiotics, Antitubercular / Q000494:pharmacology; D001483:Base Sequence; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D012293:Rifampin / Q000494:pharmacology; D014376:Tuberculosis / Q000188:drug therapy / Q000382:microbiology
D000904:Antibiotics, Antitubercular; D004269:DNA, Bacterial; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit; D012293:Rifampin