Change of conduction velocity by regional myelination yields constant latency irrespective of distance between thalamus and cortex.
The widely spanning sensory cortex receives inputs from the disproportionately smaller nucleus of the thalamus, which results in a wide variety of travelling distance among thalamic afferents. Yet, latency from the thalamus to a cortical cell is remarkably constant across the cortex (typically, approximately 2 ms). Here, we found a mechanism that produces invariability of latency among thalamocortical afferents, irrespective of the variability of travelling distances. The conduction velocity (CV) was calculated from excitatory postsynaptic currents recorded from layer IV cells in mouse thalamocortical slices by stimulating the ventrobasal nucleus of the thalamus (VB) and white matter (WM). In adults, the obtained CV for VB to WM (CV(VB-WM); 3.28 +/- 0.11 ms) was approximately 10 times faster than that of WM to layer IV cells (CV(WM-IV); 0.33 +/- 0.05 ms). The CV(VB-WM) was confirmed by recording antidromic single-unit responses from VB cells by stimulating WM. Exclusion of synaptic delay from CV(WM-IV) did not account for the 10-fold difference of CV. By histochemical staining, it was revealed that VB to WM was heavily myelinated, whereas in the cortex staining became substantially weaker. We also found that such morphological and physiological characteristics developed in parallel and were accomplished around postnatal week 4. Considering that VB to WM is longer and more variable in length among afferents than is the intracortical region, such an enormous difference of CV makes conduction time heavily dependent on the length of intracortical region, which is relatively constant. Our finding may well provide a general strategy of connecting multiple sites irrespective of distances in the brain.
Division of Neurophysiology D14, Osaka University Graduate School of Medicine, Core Research for Evolutional Science and Technology-Japan Science and Technology Corporation, 2-2 Yamadaoka, Suita 565-0871, Japan.
Mahmoud
M
Salami
Chiaki
C
Itami
Tadaharu
T
Tsumoto
Fumitaka
F
Kimura
D000375:Aging / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn; D002540:Cerebral Cortex / Q000254:growth & development / Q000502:physiology*; D004558:Electric Stimulation; D051379:Mice; D008810:Mice, Inbred C57BL; D009186:Myelin Sheath / Q000502:physiology* / Q000648:ultrastructure; D009413:Nerve Fibers, Myelinated / Q000502:physiology* / Q000648:ultrastructure; D011930:Reaction Time / Q000502:physiology*; D013788:Thalamus / Q000254:growth & development / Q000502:physiology*