Rapid high-performance liquid chromatographic measurement of amisulpride in human plasma: application to manage acute intoxication.
Amisulpride, a substituted benzamide derivative, is a second-generation (atypical) antipsychotic and is effective as maintenance therapy in patients with schizophrenia. For toxicological purpose, a rapid RP-HPLC assay was developed for the determination of amisulpride in human plasma. A linear response was observed over the concentration range 100-1000 ng/ml. A good accuracy (< or =5%) was achieved for all quality controls, with intra- and inter-day variation coefficients equal or inferior to 4.9%. The lower limit of quantification was 20 ng/ml, without interferences of endogenous components. This rapid method (run time <5 min) was used to monitor eight intoxications involving amisulpride.
Laboratoire de Pharmacologie, Université Bordeaux 2 Victor Segalen, 146 Rue Léo Saignat, 33076 Cedex, Bordeaux, France. fabienne.pehourcq@pharmaco.u-bordeaux2.fr
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Péhourcq
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Ouariki
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Bégaud
D000077582:Amisulpride; D014150:Antipsychotic Agents / Q000097:blood* / Q000506:poisoning; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013469:Sulpiride / Q000031:analogs & derivatives* / Q000097:blood* / Q000506:poisoning
D014150:Antipsychotic Agents; D013469:Sulpiride; D000077582:Amisulpride