CD9-alpha6beta1 interactions in migratory parietal endoderm cells.
Tetraspanins modulate the function of a variety of membrane proteins, including integrin receptors. We show here that the tetraspanin CD9 preferentially coimmunoprecipitates with the alpha6beta1 integrin heterodimer in F9-derived parietal endoderm cells in comparison to F9 stem cells. We also show that CD9 function-blocking antibody inhibits parietal endoderm migration in an embryoid body outgrowth assay. In addition, both CD9 and alpha6beta1 colocalize with vinculin to apparent focal adhesion sites in parietal endoderm cells. The data presented here suggests a role for CD9 in localizing the integrin to the focal adhesion. In addition, the data suggest a role for CD9 in alpha6beta1 mediated migration of parietal endoderm.
Department of Biological Sciences, Central Connecticut State University, New Britain, CT 06050, USA.
James P
JP
Mulrooney
Jessica
J
Allen
Eric
E
Bickelhaupt
Laura B
LB
Grabel
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D015703:Antigens, CD / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D002465:Cell Movement / Q000502:physiology*; D019281:Dimerization; D004707:Endoderm / Q000166:cytology / Q000378:metabolism*; D022001:Focal Adhesions / Q000378:metabolism*; D039121:Integrin alpha6beta1 / Q000378:metabolism*; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051379:Mice; D014411:Neoplastic Stem Cells; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D060245:Tetraspanin 29; D016596:Vinculin / Q000378:metabolism
D000906:Antibodies; D015703:Antigens, CD; C554870:Cd9 protein, mouse; D039121:Integrin alpha6beta1; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins; D060245:Tetraspanin 29; D016596:Vinculin