Detection of Myxobolus rotundus (Myxozoa: Myxosporea) in skin mucus of crucian carp Carassius auratus auratus using a monoclonal antibody.
Diagnosis of myxosporean Myxobolus rotundus infection was conducted by examining skin mucus from the infected crucian carp Carassius auratus auratus with a monoclonal antibody, MAb 2D12, raised previously against the parasite. A positive reaction was observed in skin mucus collected from infected fish, and spores and pre-spore stages of the parasite were identified by the MAb 2D12. It was also demonstrated that M. rotundus infection can be successfully detected by a simple method, enzyme-linked immunosorbent assay (ELISA), and that skin mucus collected from infected fish skin had a significantly higher optical density (OD) value than that from uninfected fish.
State Key Laboratory of Freshwater Ecology and Biotechnology and Laboratory of Fish Diseases, Institute of Hydrobiology,Chinese Academy of Sciences, Wuhan 430072, Hubei Province, PR China.
Y S
YS
Lu
P
P
Nie
B J
BJ
Sun
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000913:Antibodies, Protozoan / Q000276:immunology; D002347:Carps; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D056890:Eukaryota / Q000276:immunology* / Q000302:isolation & purification; D005393:Fish Diseases / Q000175:diagnosis* / Q000469:parasitology / Q000473:pathology; D009093:Mucus / Q000469:parasitology; D011529:Protozoan Infections, Animal / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D012867:Skin / Q000469:parasitology / Q000473:pathology
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan