cDNA cloning of a Rab5 homologue in Babesia gibsoni.
For studying protein trafficking in Babesia-infected erythrocyte, we describe the cloning of a Rab5, one of molecular marker for vesicle trafficking in eukaryotic cells, gene homologue in Babesia gibsoni (BgRab5). The full-length cDNA of BgRab5 is 1,020 bp long with an open reading frame encoding a protein of 220 amino acids. The deduced amino acid sequence of BgRab5 contained the highly conserved GTP-binding consensus sequence and shares about 40% homology with that of Rab5 from Plasmodium falciparum, Toxoplasma gondii, Dog, Lotus japonicusor, Oryza sativa. Northern blot analysis showed that the BgRab5 probe hybridized with a 1kb band in total RNA from parasitized erythrocytes, that was consistent with the size of the BgRab5 full-length cDNA.
Zhou
Jinlin
J
Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Ohashi
Kazuhiko
K
Yamasaki
Masahiro
M
Onuma
Misao
M
D000595:Amino Acid Sequence; D000818:Animals; D001403:Babesia / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D016384:Consensus Sequence; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D004285:Dogs; D008969:Molecular Sequence Data; D016415:Sequence Alignment / Q000662:veterinary; D017386:Sequence Homology, Amino Acid; D020696:rab5 GTP-Binding Proteins / Q000737:chemistry* / Q000235:genetics
D018076:DNA, Complementary; D020696:rab5 GTP-Binding Proteins