Preparation of hydroxyapatite-gelatin nanocomposite.
A nanocomposite of gelatin[GEL]-hydroxyapatite[HAp] was prepared using the biomimetic process. The hydroxyapatite nanocrystals were precipitated in aqueous solution of gelatin at pH 8 and 38 degrees C. The chemical bonding between calcium ions of HAp and carboxyl ions of GEL molecules induced a red-shift of the 1339 cm(-1) band of GEL in FT-IR analysis. TEM images and electron diffraction patterns for the nanocomposite strongly indicate the self-organization of HAp nanocrystals along the GEL fibrils. Electron diffraction for the nanocomposites showed a strong preferred orientation of the (002) plane in HAp nanocrystals. The development of HAp nanocrystals in an aqueous GEL solution was highly influenced by the concentration ratio of GEL to HAp. A higher concentration of GEL induced the formation of tiny crystallites (4 nm x 9 nm size), while a lower concentration of GEL contributed to the development of bigger crystallites (30 nm x 70 nm size). From DT/TGA data, the HAp-GEL nanocomposite showed typically three exothermic temperatures. The increase in decomposition temperatures indicates the formation of a primary chemical bond between HAp and GEL. The higher concentration of GEL supplies abundant reaction sites containing groups such as carboxyl, which can bind with calcium ions. The abundant supply of reaction sites leads to a very large number of HAp nuclei. However, the formation of a large number of nuclei depletes the concentration of calcium ions that available for growth to the extent that the nuclei cannot grow very large. This in turn will lead to the creation of a large number of tiny nanocrystals at this higher GEL concentration.
MDRCBB, School of Dentistry, University of Minnesota, 16-280 Moos Tower, 515 Delaware S. E. Minneapolis, MN 55455-0329, USA. mcchang@kunsan.ac.kr
Myung Chul
MC
Chang
Ching Chang
CC
Ko
William H
WH
Douglas
D040761:Biomimetic Materials / Q000138:chemical synthesis / Q000737:chemistry*; D032701:Biomimetics / Q000379:methods*; D018786:Bone Substitutes / Q000138:chemical synthesis / Q000737:chemistry*; D003460:Crystallization / Q000379:methods; D017886:Durapatite / Q000138:chemical synthesis / Q000737:chemistry*; D005780:Gelatin / Q000138:chemical synthesis / Q000737:chemistry*; D008420:Manufactured Materials; D008422:Materials Testing; D008968:Molecular Conformation; D036103:Nanotechnology / Q000379:methods*; D013499:Surface Properties
D018786:Bone Substitutes; D005780:Gelatin; D017886:Durapatite