Delayed-type hypersensitivity in sheep induced by synthetic peptides of bovine leukemia virus encapsulated in mannan-coated liposome.
Four sheep were immunized with synthesized peptides, derived from bovine leukemia virus (BLV) envelope glycoprotein, encapsulated in mannan coated liposomes as adjuvant. On the seventh day after the immunization, the sheep were intradermally challenged with BLV antigen, or synthesized peptides. The areas challenged with antigen were increased skin thickness and biopsied sequentially for immunohistological examinations. Strong delayed-type hypersensitivity was induced in sheep immunized and challenged with peptides encapsulated in mannan-coated liposomes. The major phenotype of the infiltrating lymphocytes was CD5(+). The ratio of CD4(+) to CD8(+) cells was about 1:1.
Okada
Kosuke
K
Department of Veterinary Pathology, Faculty of Agriculture, Iwate University, Ueda, Morioka, Japan.
Sonoda
Kai
K
Koyama
Masato
M
Yin
Shanai
S
Ikeda
Manabu
M
Goryo
Masanobu
M
Chen
Sau Leok
SL
Kabeya
Hidenori
H
Ohishi
Kazue
K
Onuma
Misao
M
D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D006968:Hypersensitivity, Delayed / Q000276:immunology / Q000662:veterinary*; D016130:Immunophenotyping / Q000662:veterinary; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D008081:Liposomes; D008351:Mannans / Q000008:administration & dosage / Q000276:immunology; D010455:Peptides / Q000008:administration & dosage / Q000276:immunology; D012756:Sheep / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology*
D000276:Adjuvants, Immunologic; D008081:Liposomes; D008351:Mannans; D010455:Peptides; D014759:Viral Envelope Proteins