Protective effect of aqueous garlic extract against oxidative organ damage in a rat model of thermal injury.
Oxygen free radicals have been implicated in mediating various pathological processes including burn-induced organ damage. This study was designed to determine the possible protective effect of aqueous garlic extract against oxidative organ damage distant from the original burn wound. Under ether anaesthesia, rats were subjected to severe skin scald injury covering 30% of total body surface area. Rats were decapitated either 2 h or 24 h after burn injury. Aqueous garlic extract (1 ml/kg) was administered i.p. immediately after burn injury. In the 24-h burn group injection was repeated once more (at 12 hour) following the burn injury. Liver, intestine and lung tissues were taken for the determination of malondialdehyde (MDA) and glutathione (GSH) levels, myeloperoxidase (MPO) activity and protein oxidation (PO). Burn injury caused a significant decrease in GSH level, and significant increases in MDA and PO levels, and MPO activity at post-burn 2 and 24 hours. Since garlic extract reversed these oxidant responses it seems likely that garlic extract protects tissues against oxidative damage.
Sener
Göksel
G
Marmara University, School of Pharmacy, Department of Pharmacology, Istanbul, Turkey.
Satýroğlu
Handan
H
Ozer Sehirli
A
A
Kaçmaz
Ayhan
A
D000818:Animals; D002056:Burns / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005609:Free Radicals / Q000378:metabolism; D005737:Garlic; D005978:Glutathione / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000201:enzymology; D015227:Lipid Peroxidation; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology; D011506:Proteins / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D016166:Free Radical Scavengers; D005609:Free Radicals; D010936:Plant Extracts; D011506:Proteins; D008315:Malondialdehyde; D009195:Peroxidase; D005978:Glutathione