Increased electron donor and electron acceptor characters enhance the adhesion between oil droplets and cells of Yarrowia lipolytica as evaluated by a new cytometric assay.
The adhesion of methyl ricinoleate droplets to cells of the yeast Yarrowia lipolytica was investigated. A new cytometric method, relying on the double staining of fatty globules with Nile Red and of cells with Calcofluor, enabled us to quantify methyl ricinoleate droplet adhesion to cells precultured on a hydrophilic or on a hydrophobic carbon source. In this last case, droplet adsorption was enhanced and a MATS (microbial adhesion to solvents) test revealed that this increase was due to Lewis acid-base interactions and not to an increase in the hydrophobic properties of the cell surface. These preliminary results demonstrate that the developed cytometric method is promising for various applications concerning the study of interactions between microorganisms and an emulsified hydrophobic substrates.
Aguedo
Mario
M
Laboratoire de Microbiologie, UMR UB/INRA 1082, ENSBANA, and Centre de Cytométrie en Flux, Université de Bourgogne, 21 000 Dijon, France.
Waché
Yves
Y
Mazoyer
Virginie
V
Sequeira-Le Grand
Anabelle
A
Belin
Jean-Marc
JM
D000268:Adhesiveness; D000327:Adsorption; D004579:Electron Transport; D005227:Fatty Acids / Q000737:chemistry; D005434:Flow Cytometry; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D009821:Oils / Q000737:chemistry*; D012277:Ricinoleic Acids / Q000737:chemistry; D025062:Yarrowia / Q000737:chemistry* / Q000166:cytology*
D005227:Fatty Acids; D009821:Oils; D012277:Ricinoleic Acids; C005235:methyl ricinoleate