A miniaturized, parallel, serially diluted immunoassay for analyzing multiple antigens.
This paper describes a microfluidic immunoassay that is applicable to the parallel determination of multiple analytes and that requires only a few microliters of sample. This assay relies on a microchannel network that achieves serial dilution of analytes; this network replaces manual dilutions employed in traditional immunoassays and enables the analysis of multiple analytes simultaneously. The immunoassay was demonstrated by an analysis of concentrations of antibodies against the HIV viral proteins gp120 and gp41 in human serum.
Department of Chemistry and Chemical Biology, 12 Oxford Street, Cambridge, Massachusetts 02138, USA.
Xingyu
X
Jiang
Jessamine M K
JM
Ng
Abraham D
AD
Stroock
Stephan K W
SK
Dertinger
George M
GM
Whitesides
D000906:Antibodies / Q000032:analysis*; D000941:Antigens / Q000032:analysis*; D015200:Fluoroimmunoassay / Q000295:instrumentation / Q000379:methods*; D015483:HIV Antibodies / Q000097:blood; D007074:Immunoglobulin G / Q000032:analysis; D008832:Microchemistry / Q000295:instrumentation / Q000379:methods; D008904:Miniaturization / Q000295:instrumentation / Q000379:methods; D012710:Serum Albumin, Bovine / Q000737:chemistry
D000906:Antibodies; D000941:Antigens; D015483:HIV Antibodies; D007074:Immunoglobulin G; D012710:Serum Albumin, Bovine