[Changes in the lipid physical state in human erythrocyte membranes subjected lead exposure in vitro].
The effect of lead acetate on the physical state of membrane lipids in human erythrocytes in vitro was studied using the lipophilic fluorescence probe 1,6-diphenyl-1,3,5-hexatriene and spin probes 16-doxyl-stearate and iminoxyl palmitic acid. It was shown that 2-10 microM lead acetate causes an increase in both intensity and polarization of fluorescence of 1,6-diphenyl-1,3,5-hexatriene, indicating changes in the microviscosity of the lipid bilayer of erythrocyte membranes. Judging from the parameters of EPR spectra of 16-doxyl stearate and iminoxyl palmitic acid incorporated into erythrocyte membranes, 2-10 microM lead acetate increases the heterogeneity of the lipid bilayer in surface and deep hydrophobic layers of the erythrocyte membrane.
Institute of Photobiology, National Academy of Sciences of Belarus, ul. Akademicheskaya 27, Minsk, 220072 Belarus.
O B
OB
Oleksiuk
V S
VS
Finin
E I
EI
Slobozhanina
D003497:Cyclic N-Oxides; D004161:Diphenylhexatriene; D004578:Electron Spin Resonance Spectroscopy; D004785:Environmental Pollutants / Q000633:toxicity*; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000187:drug effects*; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes; D006801:Humans; D066298:In Vitro Techniques; D008563:Membrane Lipids / Q000737:chemistry*; D009942:Organometallic Compounds / Q000633:toxicity*; D010169:Palmitic Acids; D013050:Spectrometry, Fluorescence; D013113:Spin Labels
D003497:Cyclic N-Oxides; D004785:Environmental Pollutants; D005456:Fluorescent Dyes; D008563:Membrane Lipids; D009942:Organometallic Compounds; D010169:Palmitic Acids; D013113:Spin Labels; D004161:Diphenylhexatriene; C032363:16-nitroxystearic acid; C008261:lead acetate