[Mutation of p53 and Ki-ras gene in human fetal lung fibroblast cells in vitro by sterigmatocystin].
To explore the carcinogenic effects of sterigmatocystin(ST), one of the predominant contaminating mycotoxins in high risk areas of cancer in China, mutation of tumor suppressor gene p53 and oncogene Ki-ras in human fetal lung cells in vitro induced by ST was studied using cell culture and silver-staining PCR-SSCP methods. The results showed that, within 22 weeks of ST treatment, no abnormalities were found for both p53 and Ki-ras gene in electrophoresis. Abnormal electrophoretic migration bands were seen at exon 8 of p53 gene and Ki-ras gene in ST-treated human lung fibroblast cells 22 weeks after ST treatment. Thus, the results further confirmed the carcinogenic effects of ST on human fetal lung tissue.
Institute of Basic Medical Science, Hebei Medical University, Shijiazhuang 050017, China.
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Cao
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Wang
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Zhang
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Sun
D002273:Carcinogens / Q000633:toxicity; D005333:Fetus; D005347:Fibroblasts / Q000166:cytology; D016158:Genes, p53; D011905:Genes, ras; D006801:Humans; D008168:Lung / Q000166:cytology* / Q000378:metabolism; D009154:Mutation; D013241:Sterigmatocystin / Q000633:toxicity*
D002273:Carcinogens; D013241:Sterigmatocystin