Recruitment of HIV and its receptors to dendritic cell-T cell junctions.
Monocyte-derived dendritic cells (MDDCs) can efficiently bind and transfer HIV infectivity without themselves becoming infected. Using live-cell microscopy, we found that HIV was recruited to sites of cell contact in MDDCs. Analysis of conjugates between MDDCs and T cells revealed that, in the absence of antigen-specific signaling, the HIV receptors CD4, CCR5, and CXCR4 on the T cell were recruited to the interface while the MDDCs concentrated HIV to the same region. We propose that contact between dendritic cells and T cells facilitates transmission of HIV by locally concentrating virus, receptor, and coreceptor during the formation of an infectious synapse.
McDonald
David
D
Department of Microbiology and Immunology, University of Illinois, Chicago, IL 60612, USA.
Wu
Li
L
Bohks
Stacy M
SM
KewalRamani
Vineet N
VN
Unutmaz
Derya
D
Hope
Thomas J
TJ
D015704:CD4 Antigens / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology / Q000648:ultrastructure / Q000821:virology*; D002448:Cell Adhesion; D002462:Cell Membrane / Q000821:virology; D002478:Cells, Cultured; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000502:physiology / Q000648:ultrastructure / Q000821:virology*; D005455:Fluorescent Antibody Technique; D016336:Gene Products, vpr / Q000032:analysis; D049452:Green Fluorescent Proteins; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D007365:Intercellular Junctions / Q000502:physiology / Q000821:virology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008156:Luciferases / Q000032:analysis; D008164:Luminescent Proteins / Q000032:analysis; D008954:Models, Biological; D009000:Monocytes; D019713:Receptors, CCR5 / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000378:metabolism; D015728:Receptors, HIV / Q000378:metabolism*; D014771:Virion / Q000502:physiology; D054325:vpr Gene Products, Human Immunodeficiency Virus
D015704:CD4 Antigens; D016336:Gene Products, vpr; D008070:Lipopolysaccharides; D008164:Luminescent Proteins; D019713:Receptors, CCR5; D019718:Receptors, CXCR4; D015728:Receptors, HIV; D054325:vpr Gene Products, Human Immunodeficiency Virus; D049452:Green Fluorescent Proteins; D008156:Luciferases