Cloning and characterization of a novel cardiac-specific kinase that interacts specifically with cardiac troponin I.
Cardiac-restricted genes play important roles in cardiovascular system. In an effort to identify such novel genes we identified a novel cardiac-specific kinase gene TNNI3K localized on 1p31.1 based on bioinformatics analyses. Sequence analysis suggested that TNNI3K is a distant family member of integrin-linked kinase. Northern blot and 76-tissue array analyses showed that TNNI3K is highly expressed in heart, but is undetectable in other tissues. Immunohistochemical analysis predominantly localized TNNI3K in the nucleus of cardiac myocytes. In vitro kinase assay showed that TNNI3K is a functional kinase. The yeast two-hybrid system showed that TNNI3K could directly interact with cardiac troponin I, results that were further confirmed by coimmunoprecipitation in vivo. Our data suggest that TNNI3K is a cardiac-specific kinase and play important roles in cardiac system.
Molecular Medicine Center for Cardiovascular Diseases, Cardiovascular Institute and Fu Wai Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, P.R. China.
Yong
Y
Zhao
Xian-Min
XM
Meng
Ying-Jie
YJ
Wei
Xiu-Wen
XW
Zhao
Dong-Qing
DQ
Liu
Hui-Qing
HQ
Cao
Choong-Chin
CC
Liew
Jin-Feng
JF
Ding
D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002478:Cells, Cultured; D003001:Cloning, Molecular; D005260:Female; D020869:Gene Expression Profiling; D005822:Genetic Vectors; D006801:Humans; D020930:MAP Kinase Kinase Kinases / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D009206:Myocardium / Q000201:enzymology*; D032383:Myocytes, Cardiac / Q000166:cytology; D011247:Pregnancy; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D011505:Protein-Tyrosine Kinases; D016415:Sequence Alignment; D019210:Troponin I / Q000378:metabolism*; D000099200:Scaffold Protein ILK
D020930:MAP Kinase Kinase Kinases; D017346:Protein Serine-Threonine Kinases; D011505:Protein-Tyrosine Kinases; D019210:Troponin I; D000099200:Scaffold Protein ILK; C481078:TNNI3K protein, human