Effects of static electromagnetic fields on characteristics of MG-63 osteoblasts grown in culture.
The effects of static electromagnetic fields (SEFs) on MG-63, a human osteoblast cell-line, were investigated. We examined proliferation, proline uptake and gene expression in an SEF approximately 1/728th the intensity of those previously reported. Cells were placed within an SEF apparatus (average field intensity of 0.618mT) with appropriate controls. Proliferation was measured by 3H-thymidine incorporation and showed a 34% decrease in cells exposed to SEF (P = .0001; N = 3). Proline, a major component of collagen necessary for bone formation by osteoblasts, incorporation was reduced 37% (P = 0.006; N = 3). Reverse-transcription-polymerase chain reaction revealed that collagen I, alkaline phosphatase, parathyroid hormone-receptor, and osteocalcin mRNA's were down regulated with the low intensity SEF. Exposure to very low SEFs affects the MG-63 osteoblasts in a manner that may be detrimental to bone formation.
Division of Plastic Surgery, Division of Rheumatology/Molecular Immunology, University of Mississippi Medical Center, Jackson, MS 39216-4505, USA. hcohly@surgery.umsmed.edu
Hari H P
HH
Cohly
George E
GE
Abraham
Kenneth
K
Ndebele
John J
JJ
Jenkins
James
J
Thompson
Michael F
MF
Angel
D001859:Bone Neoplasms / Q000473:pathology* / Q000503:physiopathology*; D002455:Cell Division / Q000528:radiation effects; D004574:Electromagnetic Fields; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects*; D006801:Humans; D010006:Osteoblasts / Q000166:cytology / Q000528:radiation effects; D012516:Osteosarcoma / Q000473:pathology* / Q000503:physiopathology*; D011392:Proline / Q000493:pharmacokinetics; D012016:Reference Values; D012680:Sensitivity and Specificity; D013936:Thymidine / Q000493:pharmacokinetics; D014407:Tumor Cells, Cultured
D011392:Proline; D013936:Thymidine