[Human bone marrow mesenchymal stem cells express multiple hematopoietic growth factors].
To study the biological role of human cultured bone marrow mesenchymal stem cell (BM-MSC) in hematopoiesis by investigation of its expression of multiple hematopoietic growth factors, RT-PCR was used to analyze the expression of SCF, Flt3-ligand, TPO, LIF, G-CSF, GM-CSF, IL-3, IL-6 and IL-11 at mRNA level for human BM-MSC from healthy donors and patients with leukemia and lymphoma. BM-MSC were incubated with or without hydrocortison (HC). The results clearly showed that the cultured BM-MSC expressed mRNA of SCF, Flt3-ligand, TPO, LIF, IL-6 and IL-11 at passages 3 up to 15, but did not express G-CSF, GM-CSF and IL-3. The same expression pattern of above cytokines was seen also for the patient's BM-MSC. HC was able to induce BM-MSC to express G-CSF but not to express GM-CSF. BM-MSC seemed not to change morphologically after incubation with HC for up to 21 days. In conclusion, both normal and patient BM-MSC should be potential to promote hematopoiesis according to their expression of multiple hematopoietic cytokines, and HC is able to induce hematopoietic growth factor expression.
Zhu
Guang-Rong
GR
Department of Hematology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Zhou
Xiao-Yu
XY
Lu
Hua
H
Zhou
Jian-Wei
JW
Li
Ai-Ping
AP
Xu
Wei
W
Li
Jian-Yong
JY
Wang
Cheng-Ya
CY
D001854:Bone Marrow Cells / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D016179:Granulocyte Colony-Stimulating Factor / Q000235:genetics; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics; D016298:Hematopoietic Cell Growth Factors / Q000235:genetics*; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D016298:Hematopoietic Cell Growth Factors; D012333:RNA, Messenger; D016179:Granulocyte Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006854:Hydrocortisone