Ovulation rate after GnRH or PGF2alpha administration in early postpartum dairy cows.
The objectives of this study evaluating induction of ovulation in early postpartum dairy cows were to: compare two methods of GnRH (100 mcg) administration (i.m. route and s.c. implant), and determine if prostaglandin F(2alpha) (PGF) causes release of LH or ovulation similar to that reported for GnRH. In trial #1, serum LH peaked at 2h after i.m. administration of GnRH and was declining at 4h. The s.c. GnRH implant also caused an elevation in serum LH at 2 and 4h after treatment, with LH declining at 6h. Serum LH was unchanged in control cows. Experimental treatment caused ovulation in 4 of 14 GnRH i.m. treated cows, 4 of 12 GnRH implanted cows and 0 of 13 control cows. Parity had no effect on LH response but did affect resulting ovulation rate as multiparous cows were more likely to ovulate than were primiparous cows in response to either GnRH treatment. All cows that ovulated had a follicle larger than 12 mm at the time of treatment. In trial #2, serum LH increased as before after i.m. administration of GnRH, however, serum LH was unchanged in cows treated with PGF or saline. Gonadotropin releasing hormone caused more cows to ovulate than did PGF or saline treatments, and GnRH shortened the interval from treatment to the onset of CL function over the PGF treatment; 13.9+/-2.6, 28.2+/-4.1 and 22.3+/-4.1 days for GnRH, PGF and saline, respectively. In summary, there was no difference in the ability of s.c. implantation and i.m. administration of GnRH to cause ovulation. Prostaglandin F(2alpha) did not cause release of LH or ovulation. In 22 early postpartum dairy cows treated with 100 mcg GnRH i.m. in these two trials, nearly all cows (95%) responded with a release of LH but only 45% (10/22) responded with an ovulation and subsequent formation of a CL.
Department of Clinical and Population Sciences, College of Veterinary Medicine, University of Minnesota, 435H Anim Sci/Vet Med, 1988 Fitch Avenue, St. Paul 55108, USA.
Ahmet
A
Gümen
Brad
B
Seguin
D000818:Animals; D002417:Cattle / Q000502:physiology*; D003338:Corpus Luteum / Q000502:physiology; D015237:Dinoprost / Q000008:administration & dosage*; D004343:Drug Implants; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000008:administration & dosage*; D007700:Kinetics; D007986:Luteinizing Hormone / Q000097:blood; D010060:Ovulation / Q000187:drug effects*; D010298:Parity; D049590:Postpartum Period; D013997:Time Factors
D004343:Drug Implants; D007987:Gonadotropin-Releasing Hormone; D007986:Luteinizing Hormone; D015237:Dinoprost