Effects of iron and oxygen species scavengers on Listeria spp. chemiluminescence.
Listeria monocytogenes and Listeria innocua are able, under certain conditions, to produce chemiluminescence (CL), which is amplified by luminol. Kinetic studies of CL by L. monocytogenes and L. innocua show a close parallelism between CL and growth curves during the exponential phase, with a maximum of CL reached just before entrance of bacteria into the stationary phase. CL is tightly correlated with the release of oxygen compounds. The reactive oxygen species scavengers tryptophan, mannitol, and tiron, as well as cellobiose and high temperature, were assessed with regard to CL in the two Listeria species. Only tiron strongly reduced the CL emitted by L. monocytogenes and L. innocua. On the other hand, charcoal pretreatment of the growth medium inhibited the CL, whereas ferric citrate strongly increased the CL of L. monocytogenes and L. innocua. These data suggest that iron and superoxide radical are implicated in the CL produced by these bacteria, but this phenomenon is not correlated to virulence.
Université Louis Pasteur, U.F.R. des Sciences Pharmaceutiques, Laboratoire de Bactériologie et Cryptogamie, EA 3429 and INSERM U392, 74 route du Rhin, B.P. 24, F-67401 Illkirch Cedex, France. andre@pharma.u-strasbg.fr
Philippe
P
Andre
Sandra
S
Bilger
Pauline
P
Remy
Sebastien
S
Bettinger
Dominique J-M
DJ
Vidon
D014013:1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt / Q000378:metabolism; D000818:Animals; D002455:Cell Division / Q000502:physiology; D002475:Cellobiose / Q000378:metabolism; D016166:Free Radical Scavengers / Q000378:metabolism*; D006801:Humans; D007202:Indicators and Reagents / Q000378:metabolism; D007501:Iron / Q000378:metabolism*; D008087:Listeria / Q000502:physiology*; D008163:Luminescent Measurements; D008353:Mannitol / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013696:Temperature; D014364:Tryptophan / Q000378:metabolism
D016166:Free Radical Scavengers; D007202:Indicators and Reagents; D017382:Reactive Oxygen Species; D002475:Cellobiose; D008353:Mannitol; D014013:1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt; D014364:Tryptophan; D007501:Iron