Multiplex PCR for detection of three plasmid-borne genes of enteroaggregative Escherichia coli strains.
We developed a novel multiplex PCR assay for enteroaggregative Escherichia coli (EAEC) detection, by using three plasmid-borne genes (the aggregative adherence [AA] probe, aap, and aggR). One or more of the loci were detected in 24 (86%) of 28 patient isolates analyzed. The multiplex PCR assay is a fast, convenient, and sensitive molecular test to detect EAEC.
Department of Molecular Biomedicine, CINVESTAV-IPN, Zacatenco, Mexico DF 07360, Mexico.
Jorge F
JF
Cerna
James P
JP
Nataro
Teresa
T
Estrada-Garcia
D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D029968:Escherichia coli Proteins / Q000235:genetics; D005798:Genes, Bacterial; D006801:Humans; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D015534:Trans-Activators / Q000235:genetics; D014774:Virulence / Q000235:genetics
C088603:AggR protein, E coli; D017931:DNA Primers; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D015534:Trans-Activators