Determination of polymyxin E1 in rat plasma by high-performance liquid chromatography.
A precise and accurate HPLC assay for polymyxin E(1) in rat and dog plasma has been validated. Samples and standards are extracted from plasma with a 96-well C(8) extraction disk plate. Sample extracts are derivatized with dansyl chloride, and polymyxin E(1) derivative is quantitated on a C(8) column by HPLC with fluorescence detection. The assay is linear in the range of 0.050-5.00 micro g/ml for polymyxin E(1). The precision and accuracy of polymyxin E(1) plasma assay was well within the recommended limits set in the FDA Guidance for Bioanalytical Method Validation. Polymyxin E(1) stability in rat and dog plasma for 24 h at room temperature and through three freeze-thaw cycles was demonstrated.
Gmur
Dennis J
DJ
Chiron Corporation, 201 Elliott Avenue West, Seattle, Washington 98119, USA.
Bredl
Charles R
CR
Steele
Sharon J
SJ
Cai
Shaopei
S
VanDevanter
Donald R
DR
Nardella
Pasqua A
PA
D000818:Animals; D000900:Anti-Bacterial Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003091:Colistin / Q000097:blood*; D004285:Dogs; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000900:Anti-Bacterial Agents; D003091:Colistin