HP1/ORC complex and heterochromatin assembly.
We have used the highly conserved heterochromatin component, heterochromatin protein 1 (HP1), as a molecular tag for purifying other protein components of Drosophila heterochromatin. A complex of HP1 associated with the origin recognition complex (ORC) and an HP1/ORC-associated protein (HOAP) was purified from the maternally loaded cytoplasm of early Drosophila embryo. We propose that the DNA-binding activities of ORC and HOAP function to recruit underphosphorylatedisoforms of HP1 to sites of heterochromatin nucleation. The roles of highly phosphorylated HP1, other DNA-binding proteins known to interact with HP1, and histone modifying activities in heterochromatin assembly are also addressed.
School of Biological Sciences, 101 T.H. Morgan Building, University of Kentucky, Lexington, KY 40506, USA.
Mohammed M
MM
Shareef
RamaKrishna
R
Badugu
Rebecca
R
Kellum
D000818:Animals; D000090266:Chromobox Protein Homolog 5; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics*; D002874:Chromosome Mapping; D004268:DNA-Binding Proteins / Q000235:genetics*; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics; D006570:Heterochromatin / Q000235:genetics*; D008957:Models, Genetic; D051738:Origin Recognition Complex
D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D006570:Heterochromatin; D051738:Origin Recognition Complex; C471206:cav protein, Drosophila; D000090266:Chromobox Protein Homolog 5