Detection of Coxiella burnetii by PCR in mice after administration of live M-44 vaccine.
Original primers were prepared for the detection of coxiella burnetii by PCR for the amplification of a 752-bp fragment of dnaJ gene. Using these primers we observed the persistence of C. burnetii in different organs of mice after the administration of live Q-fever vaccine over a 7-months period.
Mossienko
E V
EV
Laboratory of Zoonotic Infections, Pasteur Institute of Epidemiology and Microbiology, St. Petersburg 197 101, Russia.
Tokarevich
N K
NK
Suvorov
A N
AN
Totolian
A A
AA
D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage*; D016997:Coxiella burnetii / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016133:Polymerase Chain Reaction / Q000379:methods*; D011778:Q Fever / Q000382:microbiology / Q000517:prevention & control*; D014611:Vaccination; D014613:Vaccines, Attenuated / Q000008:administration & dosage*
D001428:Bacterial Vaccines; D004269:DNA, Bacterial; D014613:Vaccines, Attenuated