Exponential growth by cross-catalytic cleavage of deoxyribozymogens.
We have designed an autocatalytic cycle based on the highly efficient 10-23 RNA-cleaving deoxyribozyme that is capable of exponential amplification of catalysis. In this system, complementary 10-23 variants were inactivated by circularization, creating deoxyribozymogens. Upon linearization, the enzymes can act on their complements, creating a cascade in which linearized species accumulate exponentially. Seeding the system with a pool of linear catalysts resulted not only in amplification of function but in sequence selection and represents an in vitro selection experiment conducted in the absence of any protein enzymes.
Department of Chemistry and Biochemistry, Institute for Cell and Molecular Biology, University of Texas, Austin 78712, USA.
Matthew
M
Levy
Andrew D
AD
Ellington
D001483:Base Sequence; D002384:Catalysis; D004792:Enzyme Precursors / Q000378:metabolism*; D006868:Hydrolysis; D008969:Molecular Sequence Data
D004792:Enzyme Precursors