CelF of Orpinomyces PC-2 has an intron and encodes a cellulase (CelF) containing a carbohydrate-binding module.
A cDNA, designated celF, encoding a cellulase (CelF) was isolated from the anaerobic fungus Orpinomyces PC-2. The open reading frame contains regions coding for a signal peptide, a carbohydrate-binding module (CBM), a linker, and a catalytic domain. The catalytic domain was homologous to those of CelA and CelC of the same fungus and to that of the Neocallimastix patriciarum CELA, but CelF lacks a docking domain, characteristic for enzymes of cellulosomes. It was also homologous to the cellobiohydrolase IIs and endoglucanases of aerobic organisms. The gene has a 111-bp intron, located within the CBM-coding region. Some biochemical properties of the purified recombinant enzyme are described.
Chen
Huizhong
H
Center for Biological Resource Recovery, Department of Biochemistry and Molecular Biology, Life Science Building, The University of Georgia, Athens, GA 30602-7229.
Li
Xin-Liang
XL
Blum
David L
DL
Ximenes
Eduardo A
EA
Ljungdahl
Lars G
LG
D001483:Base Sequence; D001665:Binding Sites; D050260:Carbohydrate Metabolism; D020134:Catalytic Domain; D002480:Cellulase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D007438:Introns; D008969:Molecular Sequence Data; D020085:Neocallimastigales / Q000201:enzymology* / Q000235:genetics*; D016366:Open Reading Frames; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013379:Substrate Specificity
D017931:DNA Primers; D018076:DNA, Complementary; D011994:Recombinant Proteins; D002480:Cellulase