Cytokine gene polymorphisms: association with psoriatic arthritis susceptibility and severity.
OBJECTIVE
To determine whether functional cytokine gene polymorphisms influence disease susceptibility and phenotype in patients with psoriatic arthritis (PsA).


METHODS
DNA was obtained from 147 PsA patients and 389 controls. Seven functional proinflammatory (interleukin-1beta [IL-1beta] +3953, IL-6 -174, tumor necrosis factor alpha [TNFalpha] -308, TNFbeta +252) and antiinflammatory (IL-10 -1082, IL-10 -592, IL-1 receptor antagonist [intron 2, 86 bp, variable-number tandem repeat]) gene polymorphisms were detected by polymerase chain reaction and restriction fragment length polymorphism assays.


RESULTS
No significant difference in genotype frequencies was observed between the control and the PsA patient populations, and no association with Steinbrocker functional class, disease classification (polyarticular or oligoarticular), presence of spinal involvement, or age at PsA onset was observed. The presence of joint erosions was significantly associated with the TNFalpha -308 and TNFbeta +252 polymorphisms (P < 0.0001 and P = 0.0017, respectively). Frequencies of the TNFalpha -308 and TNFbeta +252 genotypes were also significantly different (P = 0.0078 and P = 0.0486, respectively) in a group of progressors (patients with early PsA in whom the number of joint erosions in the hands and feet increased over a median interval of 24 months) compared with a group of nonprogressors. Age at psoriasis onset was significantly associated with the TNFbeta +252 and TNFalpha -308 polymorphisms (P = 0.0003 and P = 0.0081, respectively). The TNFB2B2 and TNFalpha -308 AA genotypes were associated with the earliest mean ages at psoriasis onset.


CONCLUSIONS
The TNFalpha -308 and TNFbeta +252 polymorphisms were significantly associated with age at psoriasis onset, presence of joint erosions in PsA, and progression of joint erosions in early PsA. TNF gene polymorphisms may be useful prognostic markers in PsA, and these results support the rationale for using anti-TNF treatment in patients with severe, progressive PsA.
Smurfit Institute, Trinity College, Dublin, Ireland.
Joanna
J
Balding
David
D
Kane
Wendy
W
Livingstone
Lesley
L
Mynett-Johnson
Barry
B
Bresnihan
Owen
O
Smith
Oliver
O
FitzGerald
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D015535:Arthritis, Psoriatic / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D001175:Arthrography; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D004247:DNA / Q000097:blood; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D007223:Infant; D007596:Joints / Q000473:pathology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012720:Severity of Illness Index
D016207:Cytokines; D017931:DNA Primers; D004247:DNA