Identification of the bacterial etiology of culture-negative endocarditis by amplification and sequencing of a small ribosomal RNA gene.
We report two cases of culture-negative bacterial endocarditis in which the organisms were identified by amplification and sequencing of the bacterial 16S rRNA gene. These results support an important role for polymerase chain reaction followed by direct sequencing to determine the etiology of culture-negative bacterial endocarditis and to guide appropriate antimicrobial therapy.
Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
Irakli
I
Khulordava
Geraldine
G
Miller
David
D
Haas
Haijing
H
Li
Joel
J
McKinsey
Daniel
D
Vanderende
Yi-Wei
YW
Tang
D000328:Adult; D000900:Anti-Bacterial Agents; D004359:Drug Therapy, Combination / Q000008:administration & dosage; D015150:Echocardiography, Doppler; D004697:Endocarditis, Bacterial / Q000000981:diagnostic imaging / Q000188:drug therapy / Q000382:microbiology*; D005188:False Negative Reactions; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D012329:RNA, Bacterial / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000032:analysis*; D018570:Risk Assessment; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D013291:Streptococcus / Q000145:classification* / Q000235:genetics
D000900:Anti-Bacterial Agents; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S