Ultrastructural localization of phosphoglycerate kinase in adult Clonorchis sinensis.
Phosphoglycerate kinase (PGK) is an enzyme that produces one ATP molecule in the glycolytic pathway. Clonorchis sinensis is largely dependent on glycolysis for energy production. We performed immunoelectron microscopy on adult C. sinensis by using mouse immune serum raised against recombinant C. sinensis PGK. A high density of gold particles was found in the microvilli of the intestinal epithelium and in lamellae of the sperm duct. PGK was common in the somatic cells of intra-uterine eggs and in excreted products. It was localized with moderate intensity in muscular fibers of the subtegumental muscle layer, and in the myoepithelia of the intestine and excretory bladder. We suggest that PGK plays an essential role in C. sinensis energy production for movement via muscle contraction.
Department of Parasitology, Chung-Ang University College of Medicine, Tongjak-gu, 156-756, Seoul, Republic of Korea. hongsj@cau.ac.kr
Sung-Jong
SJ
Hong
Jung-Kook
JK
Shin
Shin-Yong
SY
Kang
Jae-Ran
JR
Ryu
D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology / Q000648:ultrastructure; D003004:Clonorchis sinensis / Q000201:enzymology* / Q000648:ultrastructure; D003593:Cytoplasm / Q000201:enzymology / Q000648:ultrastructure; D004734:Energy Metabolism; D004847:Epithelial Cells / Q000201:enzymology / Q000648:ultrastructure; D005260:Female; D017979:Gold Colloid; D007413:Intestinal Mucosa / Q000201:enzymology / Q000648:ultrastructure; D008297:Male; D051379:Mice; D016253:Microscopy, Immunoelectron; D018485:Muscle Fibers, Skeletal / Q000201:enzymology / Q000648:ultrastructure; D010063:Ovum / Q000201:enzymology / Q000648:ultrastructure; D010735:Phosphoglycerate Kinase / Q000032:analysis*; D011994:Recombinant Proteins; D014649:Vas Deferens / Q000201:enzymology / Q000648:ultrastructure
D017979:Gold Colloid; D011994:Recombinant Proteins; D010735:Phosphoglycerate Kinase