[Cloning and expression of the Mycoplasma hominis ftsZ for a cell division protein].
A Mycoplasma hominis chromosomal fragment containing the full-length ftsZ gene was cloned and sequenced. Natural expression of this gene was demonstrated by reverse transcription-polymerase chain reaction (RT-PCR) with total RNA. The M. hominis FtsZ protein was shown to differ substantially from its counterparts of two other Mycoplasma species, M. genitalium and M. pneumoniae. The possibility of M. hominis ftsZ expression in Escherichia coli was demonstrated with several bacterial strains. The M. hominis FtsZ protein was isolated from E. coli cells transformed with recombinant plasmids carrying the M. hominis ftsZ gene. Complementation between the E. coli and M. hominis FtsZ proteins was observed in transformants.
Institute of Physico-Chemical Medicine, Moscow, 119992 Russia.
K T
KT
Momynaliev
O V
OV
Smirnova
V N
VN
Lazarev
T A
TA
Akopian
V V
VV
Chelysheva
V M
VM
Govorun
J A
JA
Ayala
A N
AN
Simankova
S N
SN
Borkhsenius
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002455:Cell Division / Q000235:genetics; D003001:Cloning, Molecular; D003598:Cytoskeletal Proteins; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000235:genetics; D019535:Mycoplasma hominis / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D003598:Cytoskeletal Proteins; C071525:FtsZ protein, Bacteria; D011994:Recombinant Proteins