[Toxic effect of organic extracts of drinking-water on the culture luteal cells of rats].
A separation method of rat luteal cells was established. The results show that the survival rate of the cells is more than 95% during the whole operation. 14-20 days of pregnancy, 10(6) cells/ml and 4 hours of culture are the suitable conditions. The organic extracts of drinking-water produced significant dose-related increases(maximally three-fold) in progesterone concentration in the culture medium. Thus, the effects produced by the organic extracts of drinking-water in vitro may reflect the damage to the luteal cells.
School of Public Health, West China University of Medical Sciences, Chengdu 610041, China.
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D000818:Animals; D002478:Cells, Cultured; D002712:Chlorides / Q000302:isolation & purification / Q000633:toxicity; D005260:Female; D008184:Luteal Cells / Q000187:drug effects*; D011247:Pregnancy; D011374:Progesterone / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014874:Water Pollutants, Chemical / Q000302:isolation & purification / Q000633:toxicity*; D014881:Water Supply / Q000032:analysis*
D002712:Chlorides; D014874:Water Pollutants, Chemical; D011374:Progesterone