Splanchnic metabolism of dairy cows during the transition from late gestation through early lactation.
Blood flow and net nutrient fluxes for portal-drained viscera (PDV) and liver (total splanchnic tissues) were measured at 19 and 9 d prepartum and at 11, 21, 33, and 83 d in milk (DIM) in 5 multiparous Holstein-Friesian cows. Cows were fed a grass silage-based gestation ration initially and a corn silage-based lactation ration peripartum and postpartum. Meals were fed at 8-h intervals and hourly (n = 8) measures of splanchnic metabolism were started before (0730 h and 0830 h) feeding at 0830 h. Dry matter intakes (DMI) at 19 and 9 d prepartum were not different. Metabolism changes measured from 19 to 9 d prepartum were lower arterial insulin and acetate, higher arterial nonesterified fatty acids and increased net liver removal of glycerol. After calving, PDV and liver blood flow and oxygen consumption more than doubled as DMI and milk yield increased, but 85 and 93% of the respective increases in PDV and liver blood flow at 83 DIM had occurred by 11 DIM. Therefore, factors additional to DMI must also contribute to increased blood flow in early lactation. Most postpartum changes in net PDV and liver metabolism could be attributed to increases in DMI and digestion or increased milk yield and tissue energy loss. Glucose release was increasingly greater than calculated requirements as DIM increased, presumably as tissue energy balance increased. Potential contributions of lactate, alanine, and glycerol to liver glucose synthesis were greatest at 11 DIM but decreased by 83 DIM. Excluding alanine, there was no evidence of an increased contribution of amino acids to liver glucose synthesis is required in early lactation. Increased net liver removal of propionate (69%), lactate (20%), alanine (8%), and glycerol (4%) can account for increased liver glucose release in transition cows from 9 d before to 11 d after calving.
Centre for Dairy Research, Department of Agriculture, The University of Reading, Earley Gate, Reading RG6 6AT England. reynolds.345@osu.edu
C K
CK
Reynolds
P C
PC
Aikman
B
B
Lupoli
D J
DJ
Humphries
D E
DE
Beever
D020155:3-Hydroxybutyric Acid / Q000097:blood; D000085:Acetates / Q000097:blood; D000818:Animals; D001158:Arteries; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight; D002417:Cattle / Q000378:metabolism*; D004032:Diet; D004435:Eating; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005232:Fatty Acids, Volatile / Q000097:blood; D005260:Female; D005865:Gestational Age; D005990:Glycerol / Q000097:blood; D006400:Hematocrit; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000097:blood; D007774:Lactation; D008099:Liver / Q000098:blood supply; D010101:Oxygen Consumption; D006109:Poaceae; D049590:Postpartum Period; D011247:Pregnancy; D012820:Silage; D013152:Splanchnic Circulation; D014781:Viscera / Q000098:blood supply; D003313:Zea mays
D000085:Acetates; D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D005232:Fatty Acids, Volatile; D007328:Insulin; D005990:Glycerol; D020155:3-Hydroxybutyric Acid