[The study on expression of caspase-1 in experimental brain contusion of different severity in rats].
OBJECTIVE
To investigate the expression of caspase-1 in different posttraumatic intervals and severity of brain injury.


METHODS
The study examined brain tissue samples of slight (n = 24), severe (n = 24) brain injury and control (n = 6) of rat, using immunohistochemical staining, western-blot and RT-PCR method.


RESULTS
Up-regulating of caspase-1 expression was found in tissue from traumatic brain injury compared with controls in early 1 hour after TBI, and lasted for 2 weeks. Caspase-1 cleaved after brain injury.


CONCLUSIONS
These findings are the first time to demonstrate the study of different intense brain injury. The increasing of caspase-1 expression indicates that the TBI exists. The threshold area of caspase-1 positive cells is different in different severity of brain injury.
Tao
Lu-yang
LY
China Medical University, School of Forensic Medicine, Shenyang, P.R. China 110001. luyangtao@hotmail.com
Chen
Xi-ping
XP
Ding
Mei
M
D000818:Animals; D001921:Brain / Q000378:metabolism*; D001930:Brain Injuries / Q000378:metabolism*; D020170:Caspase 1 / Q000096:biosynthesis* / Q000378:metabolism; D005260:Female; D015601:Injury Severity Score; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D012333:RNA, Messenger; D020170:Caspase 1