[New improved approaches for DYS385 detection].
OBJECTIVE
To establish more sensitive methods for detection of DYS385 in routine forensic casework.


METHODS
The primers recommended by Genome Database (GDB) and Schneider were used to amplify DYS385 respectively. Then, a semi-nested PCR of DYS385 was designed by using the two different primers as outer and inner primer. A series of experiments were carried out to achieve good result by adjusting the ratio of outer/inner primer and optimizing the PCR condition.


RESULTS
It showed that an overall 112 bp shorter DYS385 fragments and better electrophoretic separation were obtained by using primer2B. By using the semi-nested PCR approach, the shorter specific DYS385 fragments could be amplified and detectable DNA amounted to 50 pg.


CONCLUSIONS
This method is 20 fold more sensitive than the ordinary method.
Faculity of Forensic Medicine, Sun Yat-sen University, Guangzhou 510089. tangshuangbo@163.net
Shuang-bo
SB
Tang
Jing-yuan
JY
Guo
Zhao-hui
ZH
Li
D041322:Chromosomes, Human, Y / Q000235:genetics*; D005554:Forensic Medicine; D005787:Gene Frequency; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods; D020080:Tandem Repeat Sequences