Isolation and characterisation of Shiga toxin-producing Escherichia coli O157:H7 from calves in Argentina.
Four hundred and twenty-two calves were examined for intestinal carriage of Shiga toxin-producing Escherichia coli O157:H7 using conventional plating. Two (0.5%) E. coli O157 were recovered. They were compared with 96 Argentine strains of different origin by pulsed-field gel electrophoresis, phage typing and PCR-RFLP of stx2 genes. One strain isolated from a calf, was closely related with 18 strains of clinical origin.
Servicio Fisiopatogenia, Instituto Nacional de Enfermedades Infecciosas - ANLIS Dr. Carlos G. Malbrán, Buenos Aires, Argentina.
I
I
Chinen
J L
JL
Otero
E S
ES
Miliwebsky
M L
ML
Roldán
A
A
Baschkier
G M
GM
Chillemi
C
C
Nóboli
L
L
Frizzo
M
M
Rivas
D000818:Animals; D001118:Argentina / Q000453:epidemiology; D001434:Bacteriophage Typing / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000382:microbiology*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D019453:Escherichia coli O157 / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D005243:Feces / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D015995:Prevalence; D022603:Shiga Toxins / Q000096:biosynthesis*
D004269:DNA, Bacterial; D022603:Shiga Toxins