Prevalence of extended-spectrum beta-lactamase-producing Gram-negative bacteria in septicaemic neonates in a tertiary care hospital.
The present study was undertaken to investigate the high incidence of multiresistant Gram-negative bacilli causing neonatal septicaemia. Samples of neonatal blood from 728 suspected cases were obtained in brain heart infusion broth with sodium polyanethol sulfonate. All Gram-negative rods isolated were subsequently subjected to routine antimicrobial susceptibility testing and tests for extended-spectrum beta-lactamase (ESBL) production, as per NCCLS recommendations. ESBL was detected in 86.6% of Klebsiella spp., 73.4% of Enterobacter spp. and 63.6% of Escherichia coli strains. It was also observed that 74.4-80.9% of these ESBL producers were resistant to cefotaxime and 47.6-59.5% were resistant to ceftazidime in routine susceptibility testing. Some ESBL producers (36.3-61.5%) were found to be susceptible to either or both cephalosporins used in this study. It is concluded that indiscriminate use of third-generation cephalosporins may be responsible for the selection of ESBL-producing multiresistant strains in the neonatal intensive-care unit (NICU).
Jain
Amita
A
Departments of Microbiology1 and Paediatrics2, King George's Medical College, Lucknow, UP -226 003, India.
Roy
Indranil
I
Departments of Microbiology1 and Paediatrics2, King George's Medical College, Lucknow, UP -226 003, India.
Gupta
Mahendra K
MK
Departments of Microbiology1 and Paediatrics2, King George's Medical College, Lucknow, UP -226 003, India.
Kumar
Mala
M
Departments of Microbiology1 and Paediatrics2, King George's Medical College, Lucknow, UP -226 003, India.
Agarwal
S K
SK
Departments of Microbiology1 and Paediatrics2, King George's Medical College, Lucknow, UP -226 003, India.
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D016470:Bacteremia / Q000453:epidemiology / Q000382:microbiology*; D004754:Enterobacter / Q000187:drug effects / Q000201:enzymology / Q000302:isolation & purification; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000302:isolation & purification; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D016905:Gram-Negative Bacterial Infections / Q000453:epidemiology / Q000382:microbiology*; D006801:Humans; D015994:Incidence; D007194:India / Q000453:epidemiology; D007231:Infant, Newborn; D007709:Klebsiella / Q000187:drug effects / Q000201:enzymology / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D015995:Prevalence; D001618:beta-Lactamases / Q000096:biosynthesis*; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D001618:beta-Lactamases