Observation of CO(2) in Fourier transform infrared spectral measurements of living Acholeplasma laidlawii cells.
In monitoring the time course of conformational disorder by Fourier transform infrared spectroscopy for intact Acholeplasma laidlawii cells grown at 37 degrees C on binary fatty acid mixtures containing oleic acid and for cells grown on pure palmitic acid, an absorption band at 2343 cm(-1) was observed. The band intensity was found to increase with time. This band was not observed in the spectra for isolated membranes. It is suggested that the 2343 cm(-1) band is due to CO(2) dissolved in water, most likely produced at the final point of fermentation of amino acid by this microorganism.
Omura
Yoko
Y
Department of Chemistry, Kanagawa Prefectural College of Nursing and Medical Technology, 1-5-1 Nakao, Asahi-ku, Yokohama 241-0815, Japan. y.omura@nifty.com
Okazaki
Norio
N
D000128:Acholeplasma laidlawii / Q000378:metabolism*; D002245:Carbon Dioxide / Q000032:analysis / Q000737:chemistry*; D002455:Cell Division; D002462:Cell Membrane / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D008956:Models, Chemical; D019301:Oleic Acid / Q000737:chemistry; D011487:Protein Conformation; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013696:Temperature; D013997:Time Factors
D002245:Carbon Dioxide; D019301:Oleic Acid; D005947:Glucose