Co-existence of apoptotic and necrotic features within one single cell as a result of menadione treatment.
In the present study we examined the effects of menadione, a redox cycling agent, on structural changes of human osteosarcoma line 143B cells. It has been previously reported that menadione can cause necrotic or apoptotic cell death in a concentration- depending manner. In our experimental model, cells were treated with 100 microM menadione for 24 hours. Using electron microscopy technique cells carrying three kinds of morphological changes were detected: necrotic cells, apoptotic cells and those demonstrating a co-existence of apoptotic and necrotic features in one single cell.
Kamiński
Marcin
M
Department of Medical Chemistry, Medical University of Gdańsk, Poland.
Karbowski
Mariusz
M
Miyazaki
Yu
Y
Kedzior
Jakub
J
Spodnik
Jan Henryk
JH
Gil
Aleksandra
A
Woźniak
Michał
M
Wakabayashi
Takashi
T
D000933:Antifibrinolytic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D008854:Microscopy, Electron; D009336:Necrosis; D012516:Osteosarcoma / Q000188:drug therapy / Q000473:pathology* / Q000648:ultrastructure; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology; D024483:Vitamin K 3 / Q000494:pharmacology*
D000933:Antifibrinolytic Agents; D024483:Vitamin K 3