K121Q PC-1 gene polymorphism is not associated with insulin resistance in a Spanish population.
OBJECTIVE
To investigate the effect of the K121Q plasma cell membrane glycoprotein (PC-1) polymorphism on the components of the insulin resistance syndrome in a population-based nationwide multicenter study in Spain.


METHODS
The subjects of the study were 293 nonrelated adults (44.7% men and 55.3% women) ages 35 to 64 years randomly chosen from a nationwide population-based survey on obesity and related conditions, including insulin resistance and cardiovascular risk factors. Obesity-related anthropometric measurements included blood pressure, oral glucose tolerance test, lipid profile (total cholesterol, high-density lipoprotein- and low-density lipoprotein-cholesterol, and triglycerides), plasma leptin, insulin levels by radioimmunoassay, and insulin resistance (homeostasis model assessment). K121Q PC-1 genotypes were determined by restriction fragment-length polymorphism-polymerase chain reaction.


RESULTS
Overall Q allele frequency was 0.14, with no differences between obese and nonobese individuals (0.15 vs. 0.13). After adjustment for sex, age, BMI, and degree of glucose tolerance, the Q allele was associated with high plasma leptin and triglyceride levels, but not with insulin resistance.


CONCLUSIONS
The results showed that the K121Q PC-1 polymorphism in the Spanish population has no significant impact on insulin sensitivity.
Department of Internal Medicine II, Hospital Clínico San Carlos, Madrid, Spain.
José Luis
JL
González-Sánchez
María Teresa
MT
Martínez-Larrad
Cristina
C
Fernández-Pérez
Agata
A
Kubaszek
Markku
M
Laakso
Manuel
M
Serrano-Ríos
D000886:Anthropometry; D003430:Cross-Sectional Studies; D005260:Female; D005951:Glucose Tolerance Test; D006801:Humans; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance / Q000235:genetics*; D020738:Leptin / Q000097:blood; D008055:Lipids / Q000097:blood; D008297:Male; D024821:Metabolic Syndrome / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D009765:Obesity / Q000235:genetics / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011755:Pyrophosphatases / Q000235:genetics*; D013030:Spain
D007328:Insulin; D020738:Leptin; D008055:Lipids; D010727:Phosphoric Diester Hydrolases; C065962:ectonucleotide pyrophosphatase phosphodiesterase 1; D011755:Pyrophosphatases