Study of the thermokinetic properties of copper(II) on Escherichia coli growth.
By using an LKB2277 Bioactivity Monitor, stop-flow mode, the power-time curves of Escherichia coli at 37 degrees C affected by Cu(II) were determined. Some parameters, such as growth rate constants k, inhibitory ratio I, the heat output Qlog in the log phase, and the generation times G were obtained. According to these parameters, we found that a low concentration of Cu(II) (0-20 microg/mL) had an promoting action on the growth of E. coli, but a high concentration of Cu(II) (40-100 microg/mL) had an inhibitory action. The toxicity of Cu(II) can also be expressed as the half-inhibitory concentration IC50; the value is 69.7 microg/mL. The assay is quantitative, inexpensive, and versatile.
Key Laboratory of Agricultural Microbiology of Chinese Agriculture Ministry, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
Yao
Y
Jun
Liu
L
Yi
Liu
L
Peng
Liang
L
Huigang
Sun
S
Ming
Qu
Q
Songsheng
Yu
Y
Ziniu
D000465:Algorithms; D002151:Calorimetry; D003300:Copper / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development*; D006358:Hot Temperature; D007700:Kinetics
D003300:Copper