Regulation of BRCA1 transcription by specific single-stranded DNA binding factors.
Since the majority of high-grade breast cancers express reduced levels of BRCA1 mRNA, we investigated the factors regulating BRCA1 transcription. Factors with specific affinity for the previously identified positive regulatory region (PRR) in the BRCA1 promoter were purified from whole-cell extracts. Identified proteins included replication protein A and a series of related factors with affinity for the sense strand of PRR. A subset of the identified factors activated the BRCA1 promoter. Identification of these families of proteins regulating the BRCA1 promoter represents an important step in the comprehension of the mechanisms responsible for breast cancer development.
Thakur
Sanjay
S
Kimmel Cancer Center, Philadelphia, Pennsylvania 19107, USA.
Nakamura
Tatsuya
T
Calin
George
G
Russo
Andrea
A
Tamburrino
Joseph F
JF
Shimizu
Masayoshi
M
Baldassarre
Gustavo
G
Battista
Sabrina
S
Fusco
Alfredo
A
Wassell
Richard P
RP
Dubois
Garret
G
Alder
Hansjuerg
H
Croce
Carlo M
CM
D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D019398:Genes, BRCA1; D006801:Humans; D008969:Molecular Sequence Data; D017354:Point Mutation; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins