Differential effects of cigarette smoke extracts on cell proliferation in gastric and colon cells.
Substantial evidence show a higher incidence of gastric cancer in smokers than nonsmokers and that cigarette smoking is highly associated with colon cancer. The present study was designed to examine the effect of cigarette smoke extracts on gastric and colon cancer cell proliferation, which is important for tumor growth. Two different cell lines were used. One was gastric cancer cell line AGS, and the other was colon cancer cell line HT-29. It was found that cigarette smoke extracts stimulated cell proliferation and c-myc expression in AGS cells. Furthermore, this proliferative action was partially blocked by the c-myc antisense. However, the extracts significantly inhibited HT-29 cell proliferation and suppressed c-myc expression. In conclusion, cigarette smoke extracts stimulated AGS cell proliferation, while inhibiting HT-29 proliferation, which were partially mediated by a c-myc-related pathway. The former action may play a contributory role in the carcinogenic action of cigarette smoking in the stomach.
Department of Pharmacology, Faculty of Medicine, University of Hong Kong, Hong Kong, HKSAR, China.
Vivian Yvonne
VY
Shin
Hong-Ying
HY
Wang
Edgar Shiu-Lam
ES
Liu
Marcel Wing-Leung
MW
Koo
Chi-Hin
CH
Cho
D000704:Analysis of Variance; D016923:Cell Death; D002455:Cell Division / Q000502:physiology*; D002470:Cell Survival; D002478:Cells, Cultured; D003110:Colonic Neoplasms / Q000473:pathology; D005753:Gastric Mucosa / Q000166:cytology*; D016259:Genes, myc; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology*; D016376:Oligonucleotides, Antisense; D012906:Smoke / Q000009:adverse effects*; D012907:Smoking / Q000009:adverse effects*; D013997:Time Factors; D014407:Tumor Cells, Cultured
D016376:Oligonucleotides, Antisense; D012906:Smoke