[Proteomics analysis and biochemical activity detection on facial nerve regeneration fluid].
OBJECTIVE
To explore the protein components and the biological neutrional activity upon motor neuron, so that take a general, basic study on the nerve regeneration microenvironment which be important to facial nerve regeneration.


METHODS
The animal model of rabbit facial regeneration microenvironment is established. Advanced technology of two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) was used to study on the structural proteins in the regeneration fluid, as well as the motor neuron culture was used to test the biological activity in it.


RESULTS
850 +/- 78 protein spots were detected in the regeneration fluid. The body area, neurite length and OD value of experimental group are higher than that of control group.


CONCLUSIONS
We got the protein image of facial nerve regeneration fluid by 2-D PAGE, and proved that many kinds and number of proteins in the regeneration fluid have neutrional activity on motor neuron.
Department of Otolaryngology, Huaxi Hospital of Sichuan University, Chengdu 610041.
Hui
H
Yang
Chuanyu
C
Liang
Bingqing
B
Gao
Xiaofeng
X
Lu
Shengfu
S
Li
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D005154:Facial Nerve / Q000737:chemistry / Q000502:physiology*; D066298:In Vitro Techniques; D009046:Motor Neurons / Q000737:chemistry / Q000502:physiology; D009416:Nerve Regeneration / Q000502:physiology*; D011506:Proteins / Q000032:analysis; D040901:Proteomics; D011817:Rabbits
D011506:Proteins