The loss of the hemoglobin H2S-binding function in annelids from sulfide-free habitats reveals molecular adaptation driven by Darwinian positive selection.
The hemoglobin of the deep-sea hydrothermal vent vestimentiferan Riftia pachyptila (annelid) is able to bind toxic hydrogen sulfide (H(2)S) to free cysteine residues and to transport it to fuel endosymbiotic sulfide-oxidising bacteria. The cysteine residues are conserved key amino acids in annelid globins living in sulfide-rich environments, but are absent in annelid globins from sulfide-free environments. Synonymous and nonsynonymous substitution analysis from two different sets of orthologous annelid globin genes from sulfide rich and sulfide free environments have been performed to understand how the sulfide-binding function of hemoglobin appeared and has been maintained during the course of evolution. This study reveals that the sites occupied by free-cysteine residues in annelids living in sulfide-rich environments and occupied by other amino acids in annelids from sulfide-free environments, have undergone positive selection in annelids from sulfide-free environments. We assumed that the high reactivity of cysteine residues became a disadvantage when H(2)S disappeared because free cysteines without their natural ligand had the capacity to interact with other blood components, disturb homeostasis, reduce fitness and thus could have been counterselected. To our knowledge, we pointed out for the first time a case of function loss driven by molecular adaptation rather than genetic drift. If constraint relaxation (H(2)S disappearance) led to the loss of the sulfide-binding function in modern annelids from sulfide-free environments, our work suggests that adaptation to sulfide-rich environments is a plesiomorphic feature, and thus that the annelid ancestor could have emerged in a sulfide-rich environment.
Equipe Evolution et Génétique des Populations Marines, Station Biologique de Roscoff, Unité Mixte de Recherche 7127, Centre National de la Recherche Scientifique, Universite Pierre et Marie Curie, 29680 Roscoff, France. bailly@sb-roscoff.fr
Xavier
X
Bailly
Riwanon
R
Leroy
Susan
S
Carney
Olivier
O
Collin
Franck
F
Zal
Andre
A
Toulmond
Didier
D
Jollivet
D000595:Amino Acid Sequence; D000818:Animals; D000845:Annelida / Q000145:classification* / Q000235:genetics*; D001665:Binding Sites; D005075:Biological Evolution; D016384:Consensus Sequence; D003545:Cysteine; D004777:Environment; D005914:Globins / Q000737:chemistry; D006454:Hemoglobins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006862:Hydrogen Sulfide / Q000378:metabolism*; D008954:Models, Biological; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012641:Selection, Genetic
D006454:Hemoglobins; D005914:Globins; D003545:Cysteine; D006862:Hydrogen Sulfide