Advances in methodology for the validation of methods according to the International Organization for Standardization. Application to the determination of benzoic and sorbic acids in soft drinks by high-performance liquid chromatography.
Robust chemometric techniques such as least median of squares regression, H15 Huber estimator and Lenth's method are fundamental tools in the validation of analytical methods since they contribute the strategies needed to estimate efficiently parameters such as robustness, linear range, selectivity, accuracy (trueness and precision) and the capability of detection. In addition, the capability of discrimination defined as a generalisation of the capability of detection for any nominal concentration is evaluated. The new strategy proposed is applied to the validation of a chromatographic method for use in systematic analysis.
García
Inmaculada
I
Department of Chemistry, Faculty of Sciences, University of Burgos, Pza. Misael Bañuelos s/n 09001, Burgos, Spain.
Ortiz
M Cruz
MC
Sarabia
Luis
L
Vilches
Carmen
C
Gredilla
Elisa
E
D019817:Benzoic Acid / Q000032:analysis*; D001628:Beverages / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D012680:Sensitivity and Specificity; D013011:Sorbic Acid / Q000032:analysis*
D019817:Benzoic Acid; D013011:Sorbic Acid