Methods for direct determination of mitomycin C in aqueous solutions and in urine.
Stripping voltammetry (SV) is used to quantitatively determine concentrations of the anti-neoplastic drug mitomycin C (MMC) alone and in mixtures with 5-fluorouracil and cisplatin, both of which are used in combined chemotherapy with MMC. If the accumulation is performed at the potentials of MMC reduction (-0.35 V vs. SCE), reduced MMC is strongly adsorbed at the electrode. It is possible to prepare a MMC-modified electrode, which, after a washing step, is transferred to the background electrolyte to determine MMC by voltammetry. This procedure, which is termed transfer stripping voltammetry (TSV), helps to eliminate interferences and can be applied for a direct determination of MMC alone or in mixtures with other drugs in urine.
Departamento de Química Física. Universidad de Alcalá, 28871 Alcalá de Henares, Madrid. Spain.Institute of Biophysics. Academy of Sciences of the Czech Republic, Královopolská 135, 61265 Brno. Czech Republic. qfdmn@quifis.alcala.es
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