Analysis of lewis antigens on cell surface and alpha1,3 fucosyltransferase subtypes in H7721 human hepatocarcinoma cells.
The expressions of Lewis antigens on H7721 human hepatocarcinoma cells were detected with monoclonal antibodies and flow-cytometry. It was found that H7721 mainly expressed SLex, and a small amount of SDLex, but Lex and SLea was negligible. The monoclonal antibody of SLex (KM93) significantly blocked the adhesion of H7721 cells to human umbilical vein epithelial cells, as well as cell migration and invasion, but the blocking effect of SDLex antibody (FH6) was not statistically significant. The expressions of five subtypes of alpha1,3fucosyltransferases (alpha1,3FucTs), the enzyme responsible for the fucosylation step in Lewis antigen synthesis, were also studied using real-time RT-PCR. The expression of FucT mRNAs were FucT-IV > FucT-III > FucT-VI > or = FucT-VII > FucT-IX. FucT-VI is supposed to be the main enzyme responsible for the synthesis SLex and SDLex. FucT-VII and III may also participate in SLex synthesis. The absence of FucT-IX expression and FucT-III not being the rate-limiting enzyme for SLea synthesis may be the reasons for the negligible expressions of Lex and SLea on the cell surface, respectively.
Key Laboratory of Glycoconjugate Research, Ministry of Health, Department of Biochemistry, School of Medicine, Fu-Dan University, Shanghai, China.
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Guo
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Zhang
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Zhang
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Chen
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Zhang
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Wang
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Narimatsu
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D017931:DNA Primers; D005434:Flow Cytometry; D005647:Fucosyltransferases / Q000235:genetics*; D006801:Humans; D007983:Lewis Blood Group Antigens / Q000235:genetics*; D008113:Liver Neoplasms; D014407:Tumor Cells, Cultured; D014471:Umbilical Veins / Q000502:physiology
D000911:Antibodies, Monoclonal; D017931:DNA Primers; D007983:Lewis Blood Group Antigens; D005647:Fucosyltransferases; C031692:galactoside 3-fucosyltransferase