Imbalance of serum cytokine network in sarcoid patients: index of sarcoidosis relapse?
OBJECTIVE
The follow-up of sarcoidosis is usually performed by invasive tools such as thoracic biopsy, supported by Broncho-Alveolar Lavage (BAL), Gallium-scintigraphy and Gadolinium Magnetic Resonance Imaging (Gd-MRI) to evaluate the degree of disease. Its pathogenesis can be ascribed to an accumulation of cells due to the disregulation of the immune system which involves Th1/Th2 cells as well as the soluble factors they generate. We evaluated serum cytokine and membrane marker levels (cytokine network) in sarcoid patients in order to study the correlation with the disease activity, in particular with the occurrence of sarcoidosis relapses.


METHODS
Seven sarcoid patients clinically stable without therapy, at different stages, were enrolled in our study. Cytokine and membrane marker serum values were measured monthly for 24 months, after a six-month period of run-in, by ELISA assay and MoAb indirect immunofluorescence, respectively.


RESULTS
In some patients at the first and second stages of the disease we observed MCP-1 and inflammatory cytokine peaks and, moreover, MCP-1 values were increased before other cytokine values. After three subsequent increases of these parameters were observed, according to our personal experience, Gadolinium-MRI confirmed the presence of an increase of the lesion and therefore our hypothesis of sarcoidosis relapse. Only one patient at stage III showed constantly elevated values of fibro-angiogenic cytokines and membrane receptors of the TNF receptor family.


CONCLUSIONS
Cytokine network monitoring could be a non-invasive means of following up the clinical course of sarcoidosis.
Salmeri
Francesca M
FM
Department of Environmental Protection, University of Messina, Messina, Italy.
Sofo
Vincenza
V
Andò
Filippo G
FG
Vastola
Maria B
MB
Crosca
Sergio
S
Bitto
Alessandra
A
Gaeta
Michele
M
Girbino
Giuseppe
G
D000328:Adult; D000368:Aged; D015415:Biomarkers; D018932:Chemokine CCL2 / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D018124:Receptors, Tumor Necrosis Factor / Q000378:metabolism*; D012129:Respiratory Function Tests; D012507:Sarcoidosis / Q000175:diagnosis / Q000276:immunology*
D015415:Biomarkers; D018932:Chemokine CCL2; D016207:Cytokines; D018124:Receptors, Tumor Necrosis Factor; D007703:Peptidyl-Dipeptidase A