Evidence for a role of MCM (mini-chromosome maintenance)5 in transcriptional repression of sub-telomeric and Ty-proximal genes in Saccharomyces cerevisiae.
The MCM (mini-chromosome maintenance) genes have a well established role in the initiation of DNA replication and in the elongation of replication forks in Saccharomyces cerevisiae. In this study we demonstrate elevated expression of sub-telomeric and Ty retrotransposon-proximal genes in two mcm5 strains. This pattern of up-regulated genes resembles the genome-wide association of MCM proteins to chromatin that was reported earlier. We link the altered gene expression in mcm5 strains to a reversal of telomere position effect (TPE) and to remodeling of sub-telomeric and Ty chromatin. We also show a suppression of the Ts phenotype of a mcm5 strain by the high copy expression of the TRA1 component of the chromatin-remodeling SAGA/ADA (SPT-ADA-GCN5 acetylase/ADAptor). We propose that MCM proteins mediate the establishment of silent chromatin domains around telomeres and Ty retrotransposons.
Department of Molecular Biology and Genetics, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
Renata
R
Dziak
David
D
Leishman
Maja
M
Radovic
Bik K
BK
Tye
Krassimir
K
Yankulov
D015152:Blotting, Northern; D018797:Cell Cycle Proteins / Q000502:physiology*; D002843:Chromatin / Q000378:metabolism; D004268:DNA-Binding Proteins; D015966:Gene Expression Regulation, Fungal; D016681:Genome, Fungal; D007700:Kinetics; D008836:Micrococcal Nuclease / Q000378:metabolism; D008957:Models, Genetic; D020411:Oligonucleotide Array Sequence Analysis; D016366:Open Reading Frames; D010641:Phenotype; D018626:Retroelements; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins; D016615:Telomere / Q000648:ultrastructure*; D013696:Temperature; D013997:Time Factors; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D015854:Up-Regulation
D018797:Cell Cycle Proteins; D002843:Chromatin; D004268:DNA-Binding Proteins; D018626:Retroelements; D029702:Schizosaccharomyces pombe Proteins; C085711:mcm5 protein, S pombe; D008836:Micrococcal Nuclease