Stable expression of MHC class I heavy chain/HLA-DO complexes at the plasma membrane.
Major histocompatibility complex (MHC) class I and II molecules present antigenic fragments to the immune system. MHC-like chaperones, like HLA-DM, HLA-DO and tapasin support peptide loading. HLA class I heavy chains require association with beta 2-microglobulin and peptide for endoplasmic reticulum (ER) exit. Likewise, HLA-DO is only able to leave the ER by association to DM. Here we show that HLA-DO and free MHC class I heavy chains associate into a stable complex early during biosynthesis and are expressed at the plasma membrane as a complex. These DO/heavy chain complexes are found on DO-transfected cells and on low amounts on human B cells.
Division of Tumor Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands.
Marcel
M
van Lith
Marieke
M
van Ham
Jacques
J
Neefjes
D001402:B-Lymphocytes / Q000502:physiology; D002460:Cell Line; D006681:HLA-D Antigens / Q000737:chemistry* / Q000502:physiology; D006207:Half-Life; D015395:Histocompatibility Antigens Class I / Q000737:chemistry* / Q000502:physiology; D006801:Humans; D011233:Precipitin Tests
D006681:HLA-D Antigens; C084245:HLA-DM antigens; C046148:HLA-DO antigens; D015395:Histocompatibility Antigens Class I