Identification of teleost Thy-1 and association with the microdomain/lipid raft reggie proteins in regenerating CNS axons.
During regeneration, retinal ganglion cell axons in fish upregulate a cell surface protein that is recognized by the monoclonal antibody (mAB) M802. M802 antigen appeared to be linked to the intracellular, membrane-associated lipid raft/microdomain proteins reggie-1 and reggie-2 that were previously shown to be reexpressed in axon-regenerating neurons [Development 124 (1997), 577]. Here, we report the isolation of the M802 antigen and its identification as the teleost homolog of mammalian Thy-1. Fish Thy-1 is detected in the same detergent-insoluble lipid raft fractions from a fibroblast cell line and from axon regenerating retinae as reggie-1 and 2. Importantly, mAB M802 coimmunoprecipitates reggie-1 and 2 from this lipid raft fraction, implying that fish Thy-1 and reggies interact. This correlates with their colocalization in growing cell processes after M802 antigen/Thy-1 activation with mAB M802. These findings suggest a role of clustered M802 antigen/Thy-1 in reggie raft microdomains for cell growth and axon regeneration.
Deininger
Soeren-Oliver
SO
Department of Chemistry, University of Konstanz, 78457 Konstanz, Germany.
Rajendran
Lawrence
L
Lottspeich
Friedrich
F
Przybylski
Michael
M
Illges
Harald
H
Stuermer
Claudia A O
CA
Reuter
Alexander
A
D000818:Animals; D001369:Axons / Q000378:metabolism* / Q000648:ultrastructure; D002478:Cells, Cultured; D002490:Central Nervous System / Q000166:cytology / Q000378:metabolism*; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D029941:Fish Proteins / Q000378:metabolism*; D006054:Goldfish / Q000033:anatomy & histology / Q000378:metabolism*; D020439:Growth Cones / Q000378:metabolism; D021962:Membrane Microdomains / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009416:Nerve Regeneration / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D018800:Thy-1 Antigens / Q000235:genetics / Q000302:isolation & purification*
D018076:DNA, Complementary; D029941:Fish Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D018800:Thy-1 Antigens; C106354:flotillins; C104436:reggie-2 protein, Carassius auratus