Functional groups on polystyrene model nanoparticles: influence on protein adsorption.
The surface characteristics of intravenously administered particulate drug carriers decisively influence the protein adsorption that is regarded as a key factor for the in vivo fate of the carriers. Latex nanoparticles were synthesized to study the influence of different basic and acidic functional groups on particulate surfaces on the protein adsorption from human serum. The protein mass adsorbed to the particles was assessed by BCA protein assay, the protein adsorption patterns were analyzed by two-dimensional electrophoresis. Considerable differences in the protein adsorption with regard to preferential adsorbed proteins were detectable for the different functional groups. Possible correlations between the surface characteristics and the protein adsorption are shown and discussed. The knowledge concerning the interactions of proteins and nanoparticles can be used for a rational development of particulate drug carriers and can also be useful for an optimized design of medical devices, e.g., hemodialysis membranes or implants.
Department of Pharmaceutics, Biopharmaceutics and Biotechnology, The Free University of Berlin, D-12169 Berlin, Germany.
Andrea
A
Gessner
Antje
A
Lieske
Bernd-R
BR
Paulke
Rainer H
RH
Müller
D000327:Adsorption; D015180:Electrophoresis, Gel, Two-Dimensional; D004655:Emulsions; D005782:Gels; D006023:Glycoproteins / Q000737:chemistry; D006454:Hemoglobins / Q000737:chemistry; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000737:chemistry; D007840:Latex / Q000737:chemistry; D008863:Microspheres; D008954:Models, Biological; D011137:Polystyrenes / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D013499:Surface Properties; D053482:beta 2-Glycoprotein I
D004655:Emulsions; D005782:Gels; D006023:Glycoproteins; D006454:Hemoglobins; D007147:Immunoglobulin Light Chains; D007840:Latex; D011137:Polystyrenes; D011506:Proteins; D053482:beta 2-Glycoprotein I