[Application of two-dimensional electrophoresis and mass spectrometry in the research of testicular proteins].
OBJECTIVE
To evaluate the application of two-dimensional electrophoresis and mass spectrometry in the research of proteins expressed in testis.


METHODS
Protein from adult ICR mouse testes was extracted by two-dimensional electrophoresis. Two protein spots were cut from the gel, then the proteins were digested in-gel by enzyme and the generated peptides were measured by matrix assisted laser desorption/ionization-time of flight mass spectrometry. The proteins were identified through database searching.


RESULTS
Two spots in Commasie Brilliant Blue-stained gel were identified as serum albumin and protein disulfide isomerase by database searching.


CONCLUSIONS
This rapid high resolution and efficient method is a very powerful way to analyze testicular proteins.
Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, Jiangsu 210029, China.
Ye-Fei
YF
Zhu
Xiang
X
Ma
Zuo-Min
ZM
Zhou
Jia-Hao
JH
Sha
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D008813:Mice, Inbred ICR; D011506:Proteins / Q000032:analysis*; D013737:Testis / Q000737:chemistry*
D011506:Proteins