Role of a Transbilayer pH Gradient in the Membrane Fusion Activity of the Influenza Virus Hemagglutinin: Use of the R18 Assay to Monitor Membrane Merging.
It had been suggested that influenza virus-mediated membrane fusion might be dependent on a pH gradient across a target membrane. We have designed experiments in which this issue could be addressed. Two populations of liposomes were prepared, both simulating the plasma membrane of target cells, but with the pH of the internal aqueous medium buffered either at pH 7.4 (physiological cytosol pH) or at pH 5.0 (endosomal pH at which influenza virus displays maximal fusion activity). By monitoring fusion using the R18 assay, we found that the internal pH of the target liposomes did not influence membrane merging as mediated by the influenza virus hemagglutinin, thus demonstrating that a transmembrane pH gradient is not required in this fusion process.
Center for Neuroscience of Coimbra and Department of Zoology. Oregon Regional Primate Research Center. Oregon Health Sciences University, Beaverton, OR. USA.Department of Biochemistry. Apartado 3126, University of Coimbra, 3000 Coimbra. Portugal. mdelima@cygnus.ci.uc.pt
João
J
Ramalho-Santos
Maria C.
MC
Pedroso De Lima