Recovery of chondrocyte metabolic activity after thermal exposure.
BACKGROUND
The relationship between temperature elevation and thermal exposure time during thermal chondroplasty has implications for cell viability and subsequent articular cartilage function.


OBJECTIVE
To characterize cartilage metabolic changes after exposure to thermal stress and to determine whether changes seen acutely are reversible.


METHODS
Controlled laboratory study.


METHODS
Human cartilage was exposed to a 45 degrees, 50 degrees, or 55 degrees C bath for up to 3 minutes. Untreated control specimens were analyzed with each group. Viability and metabolic capability of treated and untreated specimens were evaluated immediately or 1 week after thermal stress by using methylthiotetrazole conversion, (3)H-serine incorporation into protein, and (35)S-sulfate incorporation into newly synthesized proteoglycan.


RESULTS
Nonarthritic and arthritic articular cartilage metabolic activity declined with increasing thermal exposure. Articular cartilage displayed a recovery from thermal stress after exposure to the 50 degrees C but not the 55 degrees C bath. Arthritic cartilage displayed increased sensitivity with higher temperatures.


CONCLUSIONS
Understanding of the increased sensitivity to thermal stress of arthritic articular cartilage may be helpful in thermally based treatments.


CONCLUSIONS
Further correlation with the temperatures attained during thermal chondroplasty will be necessary to confirm the clinical relevance of these in vitro observations to the use of radiofrequency energy devices to treat partial-thickness chondral lesions.
Kaplan
Lee D
LD
University of Pittsburgh School of Medicine, Department of Orthopaedic Surgery, Pittsburgh, Pennsylvania 15213, USA.
Chu
Constance R
CR
Bradley
James P
JP
Fu
Freddie H
FH
Studer
Rebecca K
RK
D001178:Arthroplasty; D002358:Cartilage, Articular / Q000378:metabolism*; D006801:Humans; D006979:Hyperthermia, Induced; D007719:Knee Joint / Q000601:surgery