Modulation of mitogenic activity of fibroblast growth factors by inorganic polyphosphate.
The proliferation of normal human fibroblast cells was enhanced by the addition of inorganic polyphosphate (poly(P)) into culture media. The mitogenic activities of acidic fibroblast growth factor (FGF-1) and basic fibroblast growth factor (FGF-2) were also enhanced by poly(P). A physical interaction between poly(P) and FGF-2 was observed, and FGF-2 was both physically and functionally stabilized by poly(P). Furthermore, poly(P) facilitated the FGF-2 binding to its cell surface receptors. Because poly(P) is widely distributed in mammalian tissues, it may be a spontaneous modulator of FGFs.
Frontier Research Division, Fujirebio Inc., 51, Komiya, Hachioji, Tokyo 192-0031, Japan. tz-shiba@fujirebio.co.jp
Toshikazu
T
Shiba
Daisuke
D
Nishimura
Yumi
Y
Kawazoe
Yuichiro
Y
Onodera
Kaori
K
Tsutsumi
Rie
R
Nakamura
Minako
M
Ohshiro
D016475:3T3 Cells; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media; D016220:Fibroblast Growth Factor 1 / Q000378:metabolism / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism / Q000494:pharmacology; D005346:Fibroblast Growth Factors / Q000378:metabolism / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D051379:Mice; D008934:Mitogens / Q000378:metabolism / Q000494:pharmacology*; D011122:Polyphosphates / Q000378:metabolism / Q000494:pharmacology*; D011485:Protein Binding; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism; D051497:Receptor, Fibroblast Growth Factor, Type 2; D017468:Receptors, Fibroblast Growth Factor / Q000378:metabolism
D003470:Culture Media; D008934:Mitogens; D011122:Polyphosphates; D017468:Receptors, Fibroblast Growth Factor; D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1; D005346:Fibroblast Growth Factors; C496353:FGFR2 protein, human; C496354:Fgfr2 protein, mouse; D020794:Receptor Protein-Tyrosine Kinases; D051497:Receptor, Fibroblast Growth Factor, Type 2