Immunological assays for chemokine detection in in-vitro culture of CNS cells.
Herein we review the various methods currently in use for determining the expression of chemokines by CNS cells in vitro. Chemokine detection assays are used in conjuction with one another to provide a comprehensive, biologically relevant assessment of the chemokines which is necessary for correct data interpretation of a specific observed biological effect. The methods described include bioassays for soluble chemokine receptors, RNA extraction, RT-PCR, Real - time quantitative PCR, gene array analysis, northern blot analysis, Ribonuclease Protection assay, Flow cytometry, ELISPOT, western blot analysis, and ELISA. No single method of analysis meets the criteria for a comprehensive, biologically relevant assessment of the chemokines, therefore more than one assay might be necessary for correct data interpretation, a choice that is based on development of a scientific rationale for the method with emphasis on the reliability and relevance of the method.
Department of Medicine, Division of Allergy, Immunology and Rheumatology. 301 Multi Research Bldg., Buffalo General Hospital, 100 High Street, Buffalo, NY 14203. USA.
Supriya D.
SD
Mahajan
Stanley A.
SA
Schwartz
Madhavan P.N.
MP
Nair