Detection of a mammalian-like group A rotavirus in diarrhoeic chicken.
The present investigation describes detection of a mammalian-like electropherogroup A rotavirus in chicken with diarrhoea. This also records the first detection of a rotavirus in an avian species from India. During the investigation 75 diarrhoeic faecal samples collected from adult chicken were screened for the presence of group A rotavirus antigen by sandwich ELISA. All three samples positive for rotavirus antigen revealed 11 bands of RNA in polyacrylamide gel electrophoresis (PAGE). In contrast to avian group A rotavirus, segment 5 was found to migrate closer to 6 as is the case with mammalian group A rotaviruses. Segments 7, 8 and 9 were found to migrate as a tight triplet, which is characteristic of group A rotavirus.
Wani
S A
SA
Division of Veterinary Microbiology and Immunology, SK University of Agricultural Sciences and Technology of Kashmir (SKUAST-K), Shuhama, (Alusteng) Srinagar 190006, India. microlab@sancharnet.in
Bhat
M A
MA
Ishaq
S M
SM
Ashrafi
M A
MA
Buchh
A S
AS
Haq
M
M
D000818:Animals; D000956:Antigens, Viral / Q000378:metabolism; D002645:Chickens; D003967:Diarrhea / Q000662:veterinary* / Q000821:virology; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005243:Feces / Q000821:virology; D007194:India; D011201:Poultry Diseases / Q000821:virology*; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D012401:Rotavirus / Q000145:classification / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000662:veterinary* / Q000821:virology
D000956:Antigens, Viral; D012367:RNA, Viral