A mammalian cell regulatory agent, CeReS-18, inhibits yeast cell proliferation but not bacterial replication.
A cell regulatory sialoglycopeptide, CeReS-18, purified from intact bovine cerebral cortex cells, has exhibited the capability of reversibly inhibiting cellular DNA synthesis and the proliferation of a wide array of mammalian cells. In the present study, the effect of CeReS-18 on the proliferation of bacterial ( Bacillus cereus and Escherichia coli) and yeast ( Saccharomyces cerevisiae and Schizosaccharomyces pombe) cells was investigated. The results showed that replication and viability of the bacterial cells were not affected by CeReS-18 at any concentration tested, including 15-fold higher than that used for inhibiting mouse 3T3 cell proliferation. In contrast to bacterial cells, CeReS-18 was able to inhibit the replication of yeast cells, in a concentration-dependent, reversible manner, and the addition of calcium to the culture medium could abrogate the inhibitory effect of CeReS-18. A cytotoxic effect of CeReS-18 on both yeast cell species was observed when it was applied at higher concentrations.
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Ke
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Center for Basic Cancer Research, College of Arts and Sciences, Kansas State University, Manhattan, KS 66506, USA.
Urban
Dustin B
DB
Urban
James E
JE
Johnson
Terry C
TC
D016475:3T3 Cells / Q000187:drug effects; D000818:Animals; D001409:Bacillus cereus / Q000187:drug effects* / Q000254:growth & development; D002417:Cattle; D002453:Cell Cycle; D002455:Cell Division / Q000187:drug effects; D018844:Cyclin-Dependent Kinases / Q000037:antagonists & inhibitors; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D051379:Mice; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects*; D012568:Schizosaccharomyces / Q000166:cytology / Q000187:drug effects*; D012795:Sialoglycoproteins / Q000494:pharmacology*
C115243:CeReS-18 protein, Bos taurus; D012795:Sialoglycoproteins; D018844:Cyclin-Dependent Kinases