In vitro evaluation of antioxidant properties of Cocos nucifera Linn. water.
Tender Cocos nucifera L. (Palmacea) water (CW), variety Chandrasankara, was tested for its ability to scavenge free radicals, inhibit lipid peroxidation and protect hemoglobin from nitrite-induced oxidation. Fresh sample of CW scavenged 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenz-thiazoline-6-sulfonic acid) (ABTS) and superoxide radicals but promoted the production of hydroxyl radicals and increased lipid peroxidation. The activity was most significant for fresh samples of CW and diminished significantly upon heat, acid or alkali treatment or dialysis. Maturity of coconut drastically decreased the scavenging ability of CW against DPPH, ABTS and superoxide radicals. CW protected hemoglobin from nitrite-induced oxidation to methemoglobin when added before the autocatalytic stage of the oxidation. Acid, alkali or heat treated or dialyzed CW showed a decreased ability in protecting hemoglobin from oxidation. The scavenging ability and protection of hemoglobin from oxidation may be partly attributed to the ascorbic acid, which is an important constituent of CW. As CW is a rich source of vitamins, amino acids and enzymes, etc., more than one active principle maybe involved.
Department of Pharmacology, Collega of Pharmaceutical Sciences, Manipal-576 199, Karnataka, India.
Sudheer Kumar
SK
Mantena
Jagadish
Sridhar Reddy
SR
Badduri
Kiran Babu
KB
Siripurapu
Mazhuvancherry Kesavan
MK
Unnikrishnan
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000032:analysis; D052160:Benzothiazoles; D001713:Biphenyl Compounds / Q000378:metabolism; D003059:Cocos; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology; D006454:Hemoglobins / Q000187:drug effects / Q000378:metabolism*; D006358:Hot Temperature; D006834:Hydrazines / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D017665:Hydroxyl Radical / Q000378:metabolism; D007700:Kinetics; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008706:Methemoglobin / Q000378:metabolism; D010084:Oxidation-Reduction; D010851:Picrates; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D013451:Sulfonic Acids / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D006454:Hemoglobins; D006834:Hydrazines; D010851:Picrates; D010936:Plant Extracts; D013451:Sulfonic Acids; D013481:Superoxides; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D017665:Hydroxyl Radical; D008706:Methemoglobin; C004931:1,1-diphenyl-2-picrylhydrazyl; D001205:Ascorbic Acid