Evaluation of Proteus mirabilis structural fimbrial proteins as antigens against urinary tract infections.
Proteus mirabilis is a common cause of urinary tract infection (UTI) and produce several types of different fimbriae, including mannose-resistant/Proteus-like fimbriae, uroepithelial cell adhesin (UCA), and P. mirabilis fimbriae (PMF). Different authors have related these fimbriae with different aspects of P. mirabilis pathogenesis, although the precise role of fimbriae in UTI has not yet been elucidated. In this work we expressed and purified recombinant structural fimbrial proteins of these fimbriae (MrpA, UcaA, and PmfA) and assessed their role as protective antigens using an ascending and a haematogenous model of UTI in the mouse. MrpA protected subcutaneously immunised mice in both models, suggesting that it could be taken into account as a promising vaccine candidate against P. mirabilis UTI. UcaA could also be an interesting subunit to be studied although it only protected mice that were challenged intravenously. All subunits elicited a strong specific serum IgG response but there was no significant correlation between antibody levels and protection. Only PmfA-immunised mice elicited a significant urinary antibody response but this protein was unable to confer protection against P. mirabilis experimental challenges. These results may contribute to the development of vaccines against P. mirabilis, an important cause of complicated UTI.
Laboratorio de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Av. Italia 3318, P.O. Box 11600, Montevideo, Uruguay.
Rafael
R
Pellegrino
Umberto
U
Galvalisi
Paola
P
Scavone
Vanessa
V
Sosa
Pablo
P
Zunino
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000652:urine; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005260:Female; D036781:Fimbriae Proteins / Q000235:genetics / Q000276:immunology; D010861:Fimbriae, Bacterial / Q000235:genetics / Q000276:immunology*; D007114:Immunization; D007668:Kidney / Q000382:microbiology; D051379:Mice; D016133:Polymerase Chain Reaction; D011512:Proteus Infections / Q000276:immunology* / Q000517:prevention & control; D011513:Proteus mirabilis / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000494:pharmacology; D001743:Urinary Bladder / Q000382:microbiology; D014552:Urinary Tract Infections / Q000276:immunology* / Q000517:prevention & control
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D011994:Recombinant Proteins; C093283:UcaA protein, Proteus mirabilis; C079120:mrpA protein, Proteus mirabilis; D036781:Fimbriae Proteins