Urine antibody against human cancer antigen NY-ESO-1.
NY-ESO-1 is one of the most immunogenic tumor antigens known to date. Spontaneous humoral and cellular immune responses against NY-ESO-1 are detected in a substantial proportion of patients with NY-ESO-1 positive cancers. NY-ESO-1 serum antibody is dependent on the presence of NY-ESO-1+ cancer cells, and antibody titers correlate with the clinical development of the disease. NY-ESO-1 serum antibody is associated with detectable NY-ESO-1-specific CD8+ T cell reactivity. High titers of NY-ESO-1 serum antibodies are found in patients with advanced NY-ESO-1+ malignancies. Urine samples of seropositive patients with normal kidney function were tested for NY-ESO-1 antibody by Western blotting and enzyme-linked immunosorbent assay (ELISA). Antibodies to NY-ESO-1 were found in the urine of patients whose NY-ESO-1 serum antibody titers were 1:10,000 or higher by Western blotting. In patients with weak (positive at 1:250, negative at 1:1,000) or no reactivity, urine antibody was not detectable. No urine NY-ESO-1 antibody was found in patients without detectable NY-ESO-1 serum antibody. Our results show that urine analysis for NY-ESO-1 antibody identifies patients with strong NY-ESO-1 immunity. Urine antibody detection may also be of value in the monitoring of spontaneous and vaccine-induced immunity against other defined tumor antigens.
Jäger
Dirk
D
II. Medizinische Klinik, Hämatologie - Onkologie, Krankenhaus Nordwest, Frankfurt, Germany.
Stockert
Elisabeth
E
Karbach
Julia
J
Herrlinger
Kristina
K
Atmaca
Akin
A
Arand
Michael
M
Chen
Yao-Tseng
YT
Gnjatic
Sacha
S
Old
Lloyd J
LJ
Knuth
Alexander
A
Jäger
Elke
E
D000818:Animals; D000912:Antibodies, Neoplasm / Q000097:blood / Q000652:urine*; D000951:Antigens, Neoplasm / Q000276:immunology*; D015153:Blotting, Western; D019556:COS Cells; D006801:Humans; D008565:Membrane Proteins; D009369:Neoplasms / Q000276:immunology*; D011506:Proteins / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; C104948:CTAG1B protein, human; D008565:Membrane Proteins; D011506:Proteins