Controlled initiation of chromosomal replication in Escherichia coli requires functional Hda protein.
Regulatory inactivation of DnaA helps ensure that the Escherichia coli chromosome is replicated only once per cell cycle, through accelerated hydrolysis of active replication initiator ATP-DnaA to inactive ADP-DnaA. Analysis of deltahda strains revealed that the regulatory inactivation of DnaA component Hda is necessary for maintaining controlled initiation but not for cell growth or viability.
Department of Biochemistry and Molecular Biology, Georgetown University Medical Center, Washington, DC 20007, USA.
Johanna Eltz
JE
Camara
Kirsten
K
Skarstad
Elliott
E
Crooke
D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division / Q000235:genetics; D002876:Chromosomes, Bacterial; D004261:DNA Replication; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D005434:Flow Cytometry; D009154:Mutation; D014161:Transduction, Genetic
D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D000251:Adenosine Triphosphatases; C435211:Hda protein, E coli