Molecular typing of dengue virus type 2 in Brazil.
Strain typing is a critical tool for molecular epidemiological analysis and can provide important information about the spread of dengue viruses. Here, we performed a molecular characterization of DEN-2 viruses isolated in Brazil during 1990-2000 from geographically and temporally distinct areas in order to investigate the genetic distribution of this serotype circulating in the country. Restriction site-specific polymerase chain reaction (RSS)-PCR presented the same pattern for all 52 Brazilian samples, showing the circulation of just one DEN-2 variant. Phylogenetic analysis using progressive pairwise alignments from 240-nucleotide sequences of the E/NS1 junction in 15 isolates showed that they belong to genotype III (Jamaica genotype).
Laboratório de Flavivirus, Departmento de Virologia, Instituto Oswaldo Cruz/FIOCRUZ, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brasil. marizepm@ioc.fiocruz.br
Marize P
MP
Miagostovich
Patrícia C
PC
Sequeira
Flávia B
FB
Dos Santos
Amanda
A
Maia
Rita Maria R
RM
Nogueira
Hermann G
HG
Schatzmayr
Eva
E
Harris
Lee W
LW
Riley
D000595:Amino Acid Sequence / Q000235:genetics; D001938:Brazil; D003716:Dengue Virus / Q000145:classification / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D005838:Genotype; D006801:Humans; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D015183:Restriction Mapping
D012367:RNA, Viral