Expression of the yeast calcineurin subunits CNA1 and CNA2 during growth and hyper-osmotic stress.
CNA1 and CNA2 encode isoforms of the catalytic subunit of calcineurin, a Ser/Thr-specific phosphoprotein phosphatase regulated by Ca(2+)/calmodulin. The relative abundance of both transcripts was evaluated during growth of Saccharomyces cerevisiae in glucose by reverse-transcription polymerase chain reaction using PDA1 mRNA as a novel internal standard. CNA1 and CNA2 were concomitantly transcribed with different average expression ratios at the exponential and stationary growth phases and both showed a remarkable drop in the expression at diauxie. Prolonged hyper-osmotic shock resulted in a moderate induction of CNA1, whereas CNA2 expression was not affected.
Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Centro de Tecnologia (CT), Bloco A - Lab. 545, 21949-900 Cidade Universitária, Rio de Janeiro, Brazil.
Eduardo Mere
EM
Del Aguila
Joab Trajano
JT
Silva
Vânia Margaret Flosi
VM
Paschoalin
D019703:Calcineurin / Q000096:biosynthesis; D002135:Calcium-Binding Proteins; D015966:Gene Expression Regulation, Fungal; D006360:Heat-Shock Proteins; D009995:Osmosis / Q000502:physiology*; D010749:Phosphoprotein Phosphatases / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins
D002135:Calcium-Binding Proteins; D006360:Heat-Shock Proteins; D029701:Saccharomyces cerevisiae Proteins; C070394:CMP2 protein, S cerevisiae; C070393:CNA1 protein, S cerevisiae; D019703:Calcineurin; D010749:Phosphoprotein Phosphatases