The Genome sequence of the SARS-associated coronavirus.
We sequenced the 29,751-base genome of the severe acute respiratory syndrome (SARS)-associated coronavirus known as the Tor2 isolate. The genome sequence reveals that this coronavirus is only moderately related to other known coronaviruses, including two human coronaviruses, HCoV-OC43 and HCoV-229E. Phylogenetic analysis of the predicted viral proteins indicates that the virus does not closely resemble any of the three previously known groups of coronaviruses. The genome sequence will aid in the diagnosis of SARS virus infection in humans and potential animal hosts (using polymerase chain reaction and immunological tests), in the development of antivirals (including neutralizing antibodies), and in the identification of putative epitopes for vaccine development.
British Columbia Cancer Agency (BCCA) Genome Sciences Centre, 600 West 10th Avenue, Vancouver, British Columbia V5Z 4E6, Canada. mmarra@bccgsc.ca
Marco A
MA
Marra
Steven J M
SJ
Jones
Caroline R
CR
Astell
Robert A
RA
Holt
Angela
A
Brooks-Wilson
Yaron S N
YS
Butterfield
Jaswinder
J
Khattra
Jennifer K
JK
Asano
Sarah A
SA
Barber
Susanna Y
SY
Chan
Alison
A
Cloutier
Shaun M
SM
Coughlin
Doug
D
Freeman
Noreen
N
Girn
Obi L
OL
Griffith
Stephen R
SR
Leach
Michael
M
Mayo
Helen
H
McDonald
Stephen B
SB
Montgomery
Pawan K
PK
Pandoh
Anca S
AS
Petrescu
A Gordon
AG
Robertson
Jacqueline E
JE
Schein
Asim
A
Siddiqui
Duane E
DE
Smailus
Jeff M
JM
Stott
George S
GS
Yang
Francis
F
Plummer
Anton
A
Andonov
Harvey
H
Artsob
Nathalie
N
Bastien
Kathy
K
Bernard
Timothy F
TF
Booth
Donnie
D
Bowness
Martin
M
Czub
Michael
M
Drebot
Lisa
L
Fernando
Ramon
R
Flick
Michael
M
Garbutt
Michael
M
Gray
Allen
A
Grolla
Steven
S
Jones
Heinz
H
Feldmann
Adrienne
A
Meyers
Amin
A
Kabani
Yan
Y
Li
Susan
S
Normand
Ute
U
Stroher
Graham A
GA
Tipples
Shaun
S
Tyler
Robert
R
Vogrig
Diane
D
Ward
Brynn
B
Watson
Robert C
RC
Brunham
Mel
M
Krajden
Martin
M
Petric
Danuta M
DM
Skowronski
Chris
C
Upton
Rachel L
RL
Roper
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D017934:Coronavirus / Q000145:classification / Q000235:genetics; D000086862:Coronavirus M Proteins; D000086462:Coronavirus Nucleocapsid Proteins; D018076:DNA, Complementary; D018965:Frameshifting, Ribosomal; D016679:Genome, Viral; D006801:Humans; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics; D019590:Nucleocapsid Proteins / Q000737:chemistry / Q000235:genetics; D016366:Open Reading Frames; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D012324:RNA-Dependent RNA Polymerase / Q000737:chemistry / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D045473:Severe acute respiratory syndrome-related coronavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D045169:Severe Acute Respiratory Syndrome / Q000821:virology; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics; D014763:Viral Matrix Proteins / Q000737:chemistry / Q000235:genetics; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000086862:Coronavirus M Proteins; D000086462:Coronavirus Nucleocapsid Proteins; D018076:DNA, Complementary; C000706232:M protein, Human coronavirus OC43; C000706237:M protein, SARS-CoV; D008562:Membrane Glycoproteins; C000706247:N protein, SARS-CoV; D019590:Nucleocapsid Proteins; D012367:RNA, Viral; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins; D014763:Viral Matrix Proteins; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase