Identification of deoxynivalenol, 3-acetyldeoxynivalenol, and zearalenone in galactose oxidase-producing isolates of Fusarium graminearum.
Fusarium graminearum strains are well known for their role as plant pathogens and for their production of mycotoxins, and less known for their secretion of galactose oxidase, a well-studied and useful enzyme. Three galactose oxidase-producing isolates of F. graminearum were grown on rice to identify the production of zearalenone and trichothecenes through the use of thin layer chromatography and gas chromatography coupled to mass fragmentation. Detection and identification of deoxynivalenol, 3-acetyldeoxynivalenol, and zearalenone were accomplished.
Department of Biochemistry, State University of Maringá, Avenida Colombo, 5790, 87020-900, Maringá--PR, Brasil.
Fabricie Marcele
FM
Wilbert
Carlos
C
Kemmelmeier
D005670:Fusarium / Q000201:enzymology / Q000472:pathogenicity*; D005692:Galactose Oxidase / Q000096:biosynthesis*; D009183:Mycotoxins / Q000032:analysis*; D014255:Trichothecenes / Q000032:analysis*; D015025:Zearalenone / Q000032:analysis*
D009183:Mycotoxins; D014255:Trichothecenes; C038972:acetyldeoxynivalenol; D015025:Zearalenone; D005692:Galactose Oxidase; C007262:deoxynivalenol