Immunochemical characterization of alkaline phosphatase from the fluid of sterile and fertile Echinococcus granulosus cysts.
Alkaline phosphatase (ALP) from hydatid cyst fluid (HCF) was purified and characterized for comparison between fertile and sterile HCF. Samples were obtained from slaughtered sheep and then sterile and fertile cysts were separated. ALP was purified from aspirated cyst fluid and biochemical parameters were determined. Sera from patients with hydatid disease (15 samples) and patients with other parasitic diseases including fascioliasis (2 samples), taeniasis ( Taenia saginata, 5 samples) and also sera from uninfected controls (15 samples), were collected and used in immunoblotting experiments with ALP from sterile and fertile HCF as antigen. Our results showed that ALP activity in fertile HCF [10.75+/-3.78 (SD) U/ml) was significantly more than in sterile HCF (6.25+/-2.43 U/ml). There were also some differences between the kinetic parameters and biochemical characteristics of ALP in fertile and sterile HCF. Immunoreactive bands were clearly observed when sera from hydatid infected patients were tested with ALP from fertile HCF as the antigen. However, this method revealed no cross-reaction between purified ALP from sterile HCF and sheep liver tissue. These findings suggest that there is some variation in the immunochemical characteristics of ALP from fertile and sterile HCF.
Vatankhah
Ali
A
Parasitology Department, Pasteur Institute of Iran, Pasteur Square, 13164, Tehran, Iran. alivatankhah@hotmail.com
Assmar
Mehdi
M
Vatankhah
Gholam Reza
GR
Shokrgozar
Mohamad Ali
MA
D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D004444:Echinococcosis, Hepatic / Q000201:enzymology* / Q000469:parasitology*; D004446:Echinococcus / Q000201:enzymology* / Q000502:physiology; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004795:Enzyme Stability; D005298:Fertility; D006863:Hydrogen-Ion Concentration; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D007526:Isoelectric Point; D008099:Liver / Q000201:enzymology; D012756:Sheep
D004791:Enzyme Inhibitors; D000469:Alkaline Phosphatase