Detection of Myxobolus rotundus (Myxozoa: Myxosporea) in skin mucus of crucian carp Carassius auratus auratus using a monoclonal antibody.
Diagnosis of myxosporean Myxobolus rotundus infection was conducted by examining skin mucus from the infected crucian carp Carassius auratus auratus with a monoclonal antibody, MAb 2D12, raised previously against the parasite. A positive reaction was observed in skin mucus collected from infected fish, and spores and pre-spore stages of the parasite were identified by the MAb 2D12. It was also demonstrated that M. rotundus infection can be successfully detected by a simple method, enzyme-linked immunosorbent assay (ELISA), and that skin mucus collected from infected fish skin had a significantly higher optical density (OD) value than that from uninfected fish.
Lu
Y S
YS
State Key Laboratory of Freshwater Ecology and Biotechnology and Laboratory of Fish Diseases, Institute of Hydrobiology,Chinese Academy of Sciences, Wuhan 430072, Hubei Province, PR China.
Nie
P
P
Sun
B J
BJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000913:Antibodies, Protozoan / Q000276:immunology; D002347:Carps; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D056890:Eukaryota / Q000276:immunology* / Q000302:isolation & purification; D005393:Fish Diseases / Q000175:diagnosis* / Q000469:parasitology / Q000473:pathology; D009093:Mucus / Q000469:parasitology; D011529:Protozoan Infections, Animal / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D012867:Skin / Q000469:parasitology / Q000473:pathology
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan