DNA microarray analysis in malignant lymphomas.
Recently, DNA microarray technology has opened new avenues for the understanding of lymphomas. By hybridization of cDNA to arrays containing >10,000 different DNA fragments, this approach allows the simultaneous evaluation of the mRNA expression of thousands of genes in a single experiment. Using sophisticated bioinformatic tools, the huge amount of raw data can be clustered resulting in (1) tumor subclassification, (2) identification of pathogenetically relevant genes, or (3) biological predictors for the clinical course. This approach already has provided novel insights into different entities of B-cell non-Hodgkin's lymphomas. Genomic DNA chip hybridization (matrix-CGH) is a complementary approach focussing on genomic aberrations. In this review, we discuss the impact of this new technology both with regard to methodological aspects as well as to novel findings influencing our understanding of lymphomas.
Schwaenen
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Abteilung Innere Medizin III, Medizinische Klinik der Universität Ulm, Robert-Koch-Str. 8, 89081, Ulm, Germany.
Wessendorf
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Kestler
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Döhner
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Lichter
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Bentz
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D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D008223:Lymphoma / Q000235:genetics*; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*