New ligand exchange chiral stationary phase for the liquid chromatographic resolution of alpha- and beta-amino acids.
A new ligand exchange chiral stationary phase (CSP) has been developed by covalently bonding (R)-N,N-carboxymethyl undecyl phenylglycinol mono-sodium salt onto silica gel and applied in the resolution of alpha- and beta-amino acids. In the resolution of alpha-amino acids, the new CSP was better insome cases than the old one, which was previously developed by covalently bonding (S)-N,N-carboxymethyl undecyl leucinol mono-sodium salt onto silica gel, but worse in some other cases than the old one in terms of the separation factors (alpha). However, the new CSP wasalways much better than the old one in terms of the resolution factors (Rs). In the resolution of beta-amino acids, the new CSP was always much better than the old one in terms of both the separation and resolution factors. In an effort to characterize the new CSP, the chromatographic behaviors for the resolution of selected alpha- and beta-amino acids were investigated with the variation of the content of organic modifier and Cu(II) concentration in aqueous mobile phase and the column temperature.
Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University, Kuemjeong-Ku, Pusan 609-735, South Korea. mhhyun@pusan.ac.kr
Myung Ho
MH
Hyun
Sang Cheol
SC
Han
Sung Hee
SH
Whangbo
D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D013237:Stereoisomerism
D000596:Amino Acids