[Redistribution of cell contents between declining old and growing young leaves in spring onion].
During storage of a spring onion (Allium fistulosa var. dacong), the plant is unable to assimilate external resource, nutrient requirement of the new bud is entirely drawn from the withering leaf where both food reserves and protoplasmic constituents (i.e. organic complexes containing the essential mineral elements) are to be mobilized. Evidences were given to show that the withdrawal process of cell contents from the old part for establishing the new growth should be considered as an alternative mode or a supplementary measure of normal phloem transport of leaf photosynthates. Removal of cell contents from the declining old part to the developing new growth is of frequent occurrence in higher plants. In the present case, it followed the process of programmed cell death (PCD). That was examined and shown by electron microscopy and biochemical analysis. There appeared shrinkage of cell volume, dissolution of cell constitution and degradation of DNA. The present investigation has also shown that the withdrawal of cell contents from the declining leaf sheath of spring onion can be wholly exhaustive. It may be carried out by active movement of the partially degraded protoplasmic constituents in forms of fragments and vesicles across the tissue, until hardly anything in the cellulose framework of the dry papery sheath, except a few crystals of Ca2+. Presence of high ATPase and APase activities along the transport path also supports this view.
State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094.
Zhao Yu
ZY
Sun
Shu Qiu
SQ
Zhang
Cheng Hou
CH
Lou
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000378:metabolism*; D016923:Cell Death / Q000502:physiology; D016922:Cellular Senescence / Q000502:physiology; D018744:DNA, Plant / Q000378:metabolism; D008854:Microscopy, Electron; D019697:Onions / Q000378:metabolism* / Q000648:ultrastructure; D018515:Plant Leaves / Q000378:metabolism / Q000648:ultrastructure
D018744:DNA, Plant; D000469:Alkaline Phosphatase; D000251:Adenosine Triphosphatases