Glioma cell-associated sustained activation of the transcription factor, nuclear factor-kappa B, was inhibited by neomycin.
Experimental evidence suggests that the transcription factor nuclear factor-Kappa B (NF-kappa B) plays an important role in tumor cell invasion, apoptosis suppression and growth. Malignant glioma is one of the most intractable tumors because of its invasiveness to surrounding brain tissue. Our study investigated the role of neomycin on NF-kappa B activity in glioma cell cultures. We performed immunocytochemical analysis of cells with the antibody NF-kappa Bp65 which results show that neomycin decreases significantly the activation of NF-kappa B when added to glioma cultures for 30 min. This finding supports an important role for neomycin in glioma invasion, apoptosis and growth. Collectively, these data suggest a rationale for clinical trials with neomycin in the treatment of gliomas.
Cuevas
Pedro
P
Departamento de Investigacion, Hospital Universitario Ramon y Cajal, Universidad de Alcala de Henares, 28034 Madrid, Spain. pedro.cuevas@hrc.es
Díaz-González
Diana
D
Dujovny
Manuel
M
D000818:Animals; D005910:Glioma / Q000378:metabolism; D007150:Immunohistochemistry; D016328:NF-kappa B / Q000187:drug effects* / Q000378:metabolism; D009355:Neomycin / Q000494:pharmacology*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D051381:Rats; D014407:Tumor Cells, Cultured / Q000187:drug effects
D016328:NF-kappa B; D011500:Protein Synthesis Inhibitors; D009355:Neomycin