Carbon repression of cellobiose dehydrogenase production in the white rot fungus Trametes versicolor is mediated at the level of gene transcription.
Cellobiose dehydrogenase (CDH) production in Trametes versicolor is induced in the presence of cellulose, but decreases when additional carbon sources such as glucose and maltose are added to the fungal cultures. Using T. versicolor-specific cdh primers in a reverse transcription-polymerase chain reaction-based approach, it appears that this repression in CDH production is being mediated at the level of gene transcription. When a 1.6-kb upstream region of the T. versicolor cdh gene was cloned and sequenced, a number of putative CreA-like binding sites were observed. We propose that these sites may be involved in mediating this repressive effect, based on their similarity to the consensus [5'-SYGGRGG-3'] site for binding of the CreA and Cre1 repressor proteins.
Microbiology Department, University College Cork, National University of Ireland, Cork, Ireland.
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PC
Stapleton
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Dobson
D001483:Base Sequence; D001487:Basidiomycota / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D002237:Carbohydrate Dehydrogenases / Q000096:biosynthesis*; D002244:Carbon / Q000378:metabolism / Q000494:pharmacology*; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D015870:Gene Expression / Q000187:drug effects; D005947:Glucose; D008320:Maltose; D008969:Molecular Sequence Data; D012097:Repressor Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000187:drug effects*
D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D012097:Repressor Proteins; D008320:Maltose; D002244:Carbon; D002237:Carbohydrate Dehydrogenases; C019859:cellobiose-quinone oxidoreductase; D005947:Glucose