Morin applied in speciation of aluminium in natural waters and biological samples by reversed-phase high-performance liquid chromatography with fluorescence detection.
A reversed-phase high-performance liquid chromatographic method with fluorescence detection for the determination of labile monomeric aluminium has been developed through pre-column complexation using morin as the analytical reagent. The highly fluorescent aluminium-morin complex (excitation wavelength 418 nm, emission wavelength 490 nm) was separated on a Spherisorb ODS 2 column with an eluent consisting of 30% methanol and 70% water (pH 1.0 with perchloric acid). The most remarkable point of this protocol was that only the most toxic aluminium species, that is, free aqua-aluminium ion and its monomeric hydroxo complex ions, selectively respond among various aluminium complexes. This strategy has been successfully applied to direct fractionation of the toxic aluminium in natural waters and biological samples without any pretreatment.
State Key Laboratory of Coordination Chemistry, Department of Chemistry and Center of Materials Analysis, Nanjing University, 22 Hankou Road, 210093, Nanjing, P.R. China.
Hong-Zhen
HZ
Lian
Yu-Fen
YF
Kang
Shu-Ping
SP
Bi
Arkin
A
Yasin
Da-Lin
DL
Shao
Yi-Jun
YJ
Chen
Le-Mei
LM
Dai
Li-Ching
LC
Tian
D000535:Aluminum / Q000097:blood / Q000134:cerebrospinal fluid / Q000302:isolation & purification* / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000737:chemistry*; D005453:Fluorescence; D005618:Fresh Water / Q000032:analysis; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry; D012680:Sensitivity and Specificity; D013662:Tea / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000097:blood / Q000134:cerebrospinal fluid / Q000302:isolation & purification* / Q000652:urine; D047309:Flavones
D000535:Aluminum; D005419:Flavonoids; D007202:Indicators and Reagents; D013662:Tea; D014874:Water Pollutants, Chemical; C008548:morin; D047309:Flavones