Combined single-molecule force and fluorescence measurements for biology.
Recent advances in single-molecule techniques allow the application of force to an individual biomolecule whilst simultaneously monitoring its response using fluorescent probes. The effects of applied mechanical load on single-enzyme turnovers, biomolecular interactions and conformational changes can now be studied with nanometer precision and millisecond time resolution.
Wallace
Mark I
MI
National Institute for Medical Research, London NW7 1AA, UK.
Molloy
Justin E
JE
Trentham
David R
DR
D001695:Biology / Q000639:trends*; D031541:Fluorescence Resonance Energy Transfer / Q000639:trends*; D018625:Microscopy, Atomic Force / Q000639:trends*