Tunica interna endothelial cell kinase expression and hematopoietic and angiogenic potentials in cord blood CD34+ cells.
Tunica interna endothelial cell kinase (TEK) is expressed in both hematopoietic and endothelial cells and plays a crucial role in hematopoiesis and angiogenesis in mouse development. In humans, however, little is known about the hematopoietic and angiogenic potentials of TEK-expressing cells in umbilical cord blood (CB) cells, which originate during the human fetal period. We therefore compared the hematopoietic and angiogenic abilities of CB CD34+TEK+ and CD34+TEK- cells by using a clonogenic assay and xenotransplantation into immunodeficient NOD/SCID mice. The results showed that colony-forming cells and cells capable of repopulating in NOD/SCID mice were present in both CD34+TEK+ and CD34+TEK- cells and that the hematopoietic activities of the cell types were similar. In contrast, the potential to differentiate into endothelial cells in vivo was greater in the CD34+TEK+ cells. All NOD/SCID mice engrafted with CD34+TEK+ cells had human CD31-expressing and VE-cadherin-expressing endothelial cells in the vessels of the ischemic muscles and/ or human endothelial cells expressing CD31, kinase-insert domain-containing receptor, and endothelial nitric oxide synthase in liver sinusoidal cells, whereas such endothelial cells were detected in only 3 of the 7 recipients engrafted with CD34+TEK- cells. This result has important implications in cell therapy using CB cells for treating hematopoietic disorders and vascular diseases.
Wada
Mika
M
Department of Pediatrics, Advanced Medical Research Center, Nihon University School of Medicine, Tokyo, Japan.
Ebihara
Yasuhiro
Y
Ma
Feng
F
Yagasaki
Hiroshi
H
Ito
Mamoru
M
Takahashi
Tsuneo
T
Mugishima
Hideo
H
Takahashi
Shigeru
S
Tsuji
Kohichiro
K
D000818:Animals; D015703:Antigens, CD; D018952:Antigens, CD34; D015820:Cadherins / Q000032:analysis; D002454:Cell Differentiation; D036101:Cord Blood Stem Cell Transplantation; D004730:Endothelium, Vascular / Q000737:chemistry / Q000166:cytology; D005312:Fetal Blood; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000201:enzymology* / Q000502:physiology; D006801:Humans; D051379:Mice; D016688:Mice, Inbred NOD; D018919:Neovascularization, Physiologic; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000032:analysis; D042787:Receptor, TIE-2 / Q000032:analysis / Q000502:physiology*; D014183:Transplantation, Heterologous; D000099152:Cadherin 5
D015703:Antigens, CD; D018952:Antigens, CD34; D015820:Cadherins; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D042787:Receptor, TIE-2; D000099152:Cadherin 5