Interactions of the gastrotropic bacterium Helicobacter pylori with the leukocyte-endothelium adhesion molecules, the selectins--a preliminary report.
The deleterious effects of Helicobacter pylori infection of the stomach are largely the result of a vigorous chronic inflammatory response, and include chronic gastritis, peptic ulceration and gastric cancer. We are exploring the possibility that carbohydrate components on H. pylori contribute to the persistent inflammation through interactions with leukocyte-endothelial adhesion molecules of the host. Lipopolysaccharides of most H. pylori strains contain sequences related to the Lewis (Le(x) or Le(a)) antigens. Carbohydrate sequences of this family encompass ligands for the leukocyte-endothelium adhesion molecules of the host, namely, the E- and P-selectins, which are expressed on inflamed endothelia, and L-selectin, which is constitutively expressed on leukocytes. Here we investigate H. pylori isolates from patients with chronic gastritis, duodenal ulcer and gastric cancer for their interactions with the selectins. Our results provide unequivocal evidence of interactions of isolates from each of the diagnostic groups with E- and L-selectins.
Glycosciences Laboratory, Imperial College of Science Technology and Medicine, Northwick Park Institute for Medical Research, Watford Road, Harrow HA1 3UJ, UK. c.galustian@ic.ac.uk
Christine
C
Galustian
Nicola
N
Elviss
Henrik
H
Chart
Robert
R
Owen
Ten
T
Feizi
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004381:Duodenal Ulcer / Q000382:microbiology / Q000503:physiopathology; D019040:E-Selectin / Q000378:metabolism*; D005260:Female; D005756:Gastritis / Q000382:microbiology / Q000503:physiopathology; D016481:Helicobacter Infections / Q000382:microbiology*; D016480:Helicobacter pylori / Q000378:metabolism* / Q000472:pathogenicity; D006801:Humans; D019041:L-Selectin / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000382:microbiology / Q000503:physiopathology
D019040:E-Selectin; D008070:Lipopolysaccharides; D019041:L-Selectin