A monoclonal mouse anti-rat Ia antibody which cross reacts with a human HLA-DRw determinant.
A mouse monoclonal antibody, called MRC OX 3, which detects a polymorphic Ia determinant in the rat and cross reacts with an Ia determinant coded for by the I-A subregion in the mouse, detects a polymorphic determinant on human B cell lines. MRC OX 3 antibody binds to human B cell lines which express HLA-DRw specificities DRw 1, DRw2 and DRw6 but does not bind to those cells which only express other HLA-DRw specificities. No significant binding of MRC OX 3 antibody to either normal or mitogen stimulated human peripheral blood leukocytes, some of which are typed as HLA-DRw1, DRw2 or DRw6, could be detected. The binding of MRC OX 3 antibody to a human B cell line could be completely inhibited by pre-absorbing the antibody with purified rat Ia antigen.
MRC Cellular Immunology Unit, Sir William Dunn School of Pathology, University of Oxford, Oxford, U.K.
W R
WR
McMaster
B C
BC
Winearls
P
P
Parham
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology; D002460:Cell Line; D003429:Cross Reactions; D006684:HLA-DR Antigens / Q000276:immunology*; D015798:HLA-DR1 Antigen; D015802:HLA-DR2 Antigen; D015808:HLA-DR6 Antigen / Q000276:immunology*; D006649:Histocompatibility Antigens / Q000276:immunology* / Q000302:isolation & purification; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice / Q000276:immunology*; D008934:Mitogens / Q000494:pharmacology; D011110:Polymorphism, Genetic; D051381:Rats / Q000276:immunology*; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D006684:HLA-DR Antigens; D015798:HLA-DR1 Antigen; D015802:HLA-DR2 Antigen; D015808:HLA-DR6 Antigen; D006649:Histocompatibility Antigens; D000949:Histocompatibility Antigens Class II; D008934:Mitogens; C038353:histocompatibility antigens RT, rat