Protection of chickens against highly lethal H5N1 and H7N1 avian influenza viruses with a recombinant fowlpox virus co-expressing H5 haemagglutinin and N1 neuraminidase genes.
Inactivated whole avian influenza virus (AIV) vaccine provides protection against homologous haemagglutinin (HA) subtype virus, but poor protection against a heterologous HA virus. Moreover, it induces chickens to produce antibodies to cross-reactive antigens, especially nucleoprotein, which is limits AIV serological surveillance. In this study, a recombinant fowlpox virus co-expressing HA (H5 subtype) and NA (NI subtype)genes of AIV was evaluated for its ability to protect chickens against intramuscular challenge with a lethal dose of highly pathogenic (HP) AIV. Susceptible chickens were also vaccinated by wing-web puncture with the parent fowlpox vaccine virus. Following challenge 4 weeks later with HPAIV, all chickens vaccinated with recombinant virus were protected, while the chickens vaccinated with either the unaltered parent fowlpox vaccine virus or unvaccinated controls experienced 100% mortality following challenge. This protection was accompanied by the high levels of specific antibody to the respective components of the recombinant vaccine. The above results showed that rFPV-HA-NA could be a potential vaccine to replace current inactivated vaccines for preventing AI.
National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, P.R. China. QCHL2001@yahoo.com.cn
Chuan-Ling
CL
Qiao
Kang-Zhen
KZ
Yu
Yong-Ping
YP
Jiang
Yong-Qing
YQ
Jia
Guo-Bin
GB
Tian
Ming
M
Liu
Guo-Hua
GH
Deng
Xiu-Rong
XR
Wang
Qing-Wen
QW
Meng
Xiu-Ying
XY
Tang
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D002645:Chickens; D004274:DNA, Recombinant / Q000235:genetics; D005587:Fowlpox virus / Q000235:genetics* / Q000502:physiology; D015870:Gene Expression; D005814:Genes, Viral / Q000235:genetics; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000276:immunology*; D053124:Influenza A Virus, H5N1 Subtype; D009980:Influenza A virus / Q000145:classification* / Q000235:genetics / Q000276:immunology*; D005585:Influenza in Birds / Q000276:immunology / Q000517:prevention & control*; D009439:Neuraminidase / Q000235:genetics / Q000276:immunology*; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control; D013997:Time Factors; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*
D000914:Antibodies, Viral; D004274:DNA, Recombinant; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D014765:Viral Vaccines; C109963:hemagglutinin, avian influenza A virus; D009439:Neuraminidase