Preparation of alginic acid layers on stainless-steel substrates for biomedical applications.
This study is concerned with the blood compatibility of alginic acid layers immobilized on gamma-aminopropyltriethoxysilane (gamma-APS)-grafted stainless-steel (SUS316L). The surfaces were characterized with contact angle measurement and X-ray photoelectron spectroscopy (XPS). The blood compatibility was evaluated in terms of platelet adhesion and blood clotting time. An in vitro platelet adhesion assay indicated that only a small number of platelets adhered to substrate surfaces modified with gamma-APS and subsequently with alginic acid. Moreover, alginic-acid-immobilized SUS316L substrates had little effect on the blood clotting time. This indicated that alginic-acid-immobilized SUS316L substrates do not adsorb some blood-clotting proteins or factors, or stimulate them.
Biomaterials Laboratory, Faculty of Engineering, Okayama University, Tsushima, 700-8530, Okayama, Japan.
Tomohiko
T
Yoshioka
Kanji
K
Tsuru
Satoshi
S
Hayakawa
Akiyoshi
A
Osaka
D000464:Alginates / Q000737:chemistry*; D001709:Biotechnology / Q000379:methods; D001769:Blood; D001779:Blood Coagulation Factors / Q000378:metabolism*; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry*; D005340:Fibrinogen / Q000378:metabolism; D020723:Glucuronic Acid / Q000737:chemistry*; D006603:Hexuronic Acids / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D010949:Plasma / Q000378:metabolism*; D015539:Platelet Activation; D010973:Platelet Adhesiveness / Q000502:physiology*; D019736:Prostheses and Implants; D011516:Prothrombin / Q000378:metabolism; D013193:Stainless Steel / Q000737:chemistry*; D013499:Surface Properties; D013925:Thromboplastin / Q000378:metabolism
D000464:Alginates; D001779:Blood Coagulation Factors; D020099:Coated Materials, Biocompatible; D006603:Hexuronic Acids; D013193:Stainless Steel; D020723:Glucuronic Acid; D011516:Prothrombin; D005340:Fibrinogen; D013925:Thromboplastin