Vitamin A deficiency leads to severe functional disturbance of the intestinal epithelium enzymes associated with diarrhoea and increased bacterial translocation in gnotobiotic rats.
A disturbance of the integrity of the intestinal epithelium with an increased risk for bacterial translocation is one of the suggested factors underlying the increased incidence of infections and septicaemia during vitamin A deficiency. In the present study the effects of vitamin A deficiency on the enzymic activity of enterocytes in response to bacterial colonization with a non-pathogenic Escherichia coli strain were studied in monocolonized and conventional Wistar rats. The monocolonized, but not the conventional, vitamin A-deficient rats had markedly reduced weight compared to their pair-fed controls and presented neurological symptoms, such as hind leg weakness, tremor and slow gait. Moreover, only in the monocolonized vitamin A-deficient rats were severe diarrhoea and bacterial translocation to extraintestinal sites-mainly kidneys-detected. Measurements of enterocyte brush-border enzyme activities revealed that lactase, sucrase, gamma-glutamyltranspeptidase (GGT) and dipeptidyl peptidase IV (DPP IV) were significantly reduced in the monocolonized vitamin A-deficient rats compared to the pair-fed controls, indicating a severe functional disturbance of the enterocytes. In conventional vitamin A-deficient rats only sucrase activity was markedly lower than in the respective controls. Our observation, that the deficient vitamin A status led to a strong reduction of enterocyte enzymic activities, associated with diarrhoea and increased bacterial translocation, mainly in the gnotobiotic rats, suggests that the composition of the bacterial flora, i.e. the colonization state, has a strong influence on triggering the severity of the functional disturbances of the intestinal epithelium, and adds to the clinical manifestations of vitamin A deficiency.
Kozakova
Hana
H
Department of Immunology and Gnotobiology, Institute of Microbiology, Academy of Sciences of the Czech Republic, Novy Hradek, Czech Republic.
Hanson
Lars A
LA
Stepankova
Renata
R
Kahu
Helena
H
Dahlgren
Ulf I
UI
Wiedermann
Ursula
U
D000818:Animals; D016470:Bacteremia / Q000382:microbiology; D018988:Bacterial Translocation; D003967:Diarrhea / Q000201:enzymology / Q000382:microbiology; D020895:Enterocytes / Q000201:enzymology / Q000382:microbiology* / Q000648:ultrastructure; D004926:Escherichia coli / Q000502:physiology*; D005856:Germ-Free Life; D007583:Jejunum / Q000166:cytology / Q000201:enzymology / Q000382:microbiology; D008871:Microvilli / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar; D014802:Vitamin A Deficiency / Q000201:enzymology* / Q000382:microbiology*