Effects of estradiol and progesterone on vertebral collagen, glycosaminoglycans and phosphatases in ovariectomized adult rats.
Vertebral collagen, glycosaminoglycans (GAGs), tartrate-resistant acid phosphatase (TRAP) and alkaline phosphatase (ALP) were measured in ovariectomized (ovx) adult Wistar rats treated with estradiol (E 2 ) (10 micro g/kg BW for 35 days on alternate days, and progesterone (P 4 ) (140 micro g/kg BW for 35 days on alternate days) in E 2 + P 4 treated rats. P 4 given alone or in combination with E 2 significantly increased the levels of collagen in the vertebral bone. Neither ovx nor E 2 treatment altered the concentration of collagen in these rats. Administration of E 2 or P 4 significantly decreased the concentration of hyaluronic acid (HA), but remaining unaffected when a combination of these steroids was given. In contrast to their effect on HA, E 2 and P 4 each significantly increased the levels of chondroitin sulfate (CS) in the vertebral bone. The specific activity of ALP was decreased after ovx. E 2 and P 4 alone or in combination also registered a significant decrease in the activities of ALP and TRAP. The results suggest that E 2 and P 4 each exert definite effects on vertebral bone turnover in ovariectomized rats.
Department of Endocrinology, Institute of Basic Medical Sciences, University of Madras, Taramani, India.
V
V
Gopala Krishnan
J
J
Arunakaran
P
P
Govindarajulu
N
N
Srinivasan
D000135:Acid Phosphatase / Q000032:analysis; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D002118:Calcium / Q000097:blood; D002809:Chondroitin Sulfates / Q000032:analysis; D003094:Collagen / Q000032:analysis*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006025:Glycosaminoglycans / Q000032:analysis*; D006820:Hyaluronic Acid / Q000032:analysis; D010052:Ovariectomy; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis*; D010758:Phosphorus / Q000097:blood; D011374:Progesterone / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013131:Spine / Q000737:chemistry / Q000187:drug effects*
D006025:Glycosaminoglycans; D010758:Phosphorus; D011374:Progesterone; D004958:Estradiol; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates; D003094:Collagen; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D010744:Phosphoric Monoester Hydrolases; D002118:Calcium