p29ING4 and p28ING5 bind to p53 and p300, and enhance p53 activity.
We identified and characterized two new ING family genes, p29ING4 and p28ING5,coding for two proteins of 249 and 240 amino acids, respectively. Both p29ING4 and p28ING5 proteins have a plant homeodomain finger motif also found in other ING proteins, and which is common in proteins involved in chromatin remodeling. p29ING4 or p28ING5 overexpression resulted in a diminished colony-forming efficiency, a decreased cell population in S phase, and the induction of apoptosis in a p53-dependent manner. Both p29ING4 and p28ING5 activate the p21/waf1 promoter, and induce p21/WAF1 expression. p29ING4 and p28ING5 enhance p53 acetylation at Lys-382 residues, and physically interact with p300, a member of histone acetyl transferase complexes, and p53 in vivo. These results indicate that p29ING4 and p28ING5 may be significant modulators of p53 function.
Laboratories of Human Carcinogenesis, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892-4255, USA.
Masayuki
M
Shiseki
Makoto
M
Nagashima
Remy M
RM
Pedeux
Mariko
M
Kitahama-Shiseki
Koh
K
Miura
Shu
S
Okamura
Hitoshi
H
Onogi
Yuichiro
Y
Higashimoto
Ettore
E
Appella
Jun
J
Yokota
Curtis C
CC
Harris
D000107:Acetylation; D000123:Acetyltransferases / Q000378:metabolism*; D000595:Amino Acid Sequence; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002455:Cell Division / Q000502:physiology; D003001:Cloning, Molecular; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D006131:Growth Inhibitors / Q000235:genetics / Q000378:metabolism*; D051548:Histone Acetyltransferases; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D011485:Protein Binding; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors; D014162:Transfection; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*; D050880:p300-CBP Transcription Factors; D000099224:p300-CBP-Associated Factor
D000123:Acetyltransferases; D018797:Cell Cycle Proteins; D018076:DNA, Complementary; D006131:Growth Inhibitors; D051548:Histone Acetyltransferases; D018398:Homeodomain Proteins; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53; D025521:Tumor Suppressor Proteins; D050880:p300-CBP Transcription Factors; D000099224:p300-CBP-Associated Factor; C483230:ING4 protein, human; C508131:ING5 protein, human