Immunohistochemical demonstration of nitric oxide synthase (NOS) in the normal rat pituitary gland, estrogen-induced rat pituitary tumor and human pituitary adenomas.
Nitric oxide synthase (NOS) immunoreactivity was examined in normal rat anterior pituitary glands, estrogen-induced rat pituitary tumors and human pituitary adenomas using a polyclonal antibody reacting with all three isoforms of NOS in both species. It was found that NOS immunorectivity in pituitary glandular cells is stronger in rat experimental pituitary tumors than in normal pituitaries. NOS immunoreactivity is also detectable in all but two human pituitary adenomas and seems to negatively correlate with microvascularization.
Pawlikowski
Marek
M
Department of Experimental Endocrinology and Hormone Diagnostics, Institute of Endocrinology, Medical University, Lódź, Poland. pawlikowski.m@wp.pl
Winczyk
Katarzyna
K
Jaranowska
Maria
M
D000236:Adenoma / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002273:Carcinogens / Q000494:pharmacology; D004195:Disease Models, Animal; D004967:Estrogens / Q000494:pharmacology; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008833:Microcirculation / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D010911:Pituitary Neoplasms / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D012016:Reference Values
D014408:Biomarkers, Tumor; D002273:Carcinogens; D004967:Estrogens; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase