Protein kinase C modifications of VE-cadherin, p120, and beta-catenin contribute to endothelial barrier dysregulation induced by thrombin.
The adherens junction is a multiprotein complex consisting of the transmembrane vascular endothelial cadherin (VEC) and cytoplasmic catenins (p120, beta-catenin, plakoglobin, alpha-catenin) responsible for the maintenance of endothelial barrier function. Junctional disassembly and modifications in cadherin/catenin complex lead to increased paracellular permeability of the endothelial barrier. However, the mechanisms of junctional disassembly remain unclear. In this study, we used the proinflammatory mediator thrombin to compromise the barrier function and test the hypothesis that phosphorylation-induced alterations of VEC, beta-catenin, and p120 regulate junction disassembly and mediate the increased endothelial permeability response. The study showed that thrombin induced dephosphorylation of VEC, which is coupled to disassembly of cell-cell contacts, but VEC remained in aggregates at the plasma membrane. The cytoplasmic catenins dissociated from the VEC cytoplasmic domain in thin membrane projections formed in interendothelial gaps. We also showed that thrombin induced dephosphorylation of beta-catenin and phosphorylation of p120. Thrombin-induced interendothelial gap formation and increased endothelial permeability were blocked by protein kinase C inhibition using chelerythrine and Gö-6976 but not by LY-379196. Chelerythrine also prevented thrombin-induced phosphorylation changes of the cadherin/catenin complex. Thus the present study links posttranslational modifications of VEC, beta-catenin, and p120 to the mechanism of thrombin-induced increase in endothelial permeability.
Department of Pharmacology, University of Illinois, Chicago, IL 60612, USA.
Maria
M
Konstantoulaki
Panos
P
Kouklis
Asrar B
AB
Malik
D000470:Alkaloids; D015703:Antigens, CD; D053119:Benzophenanthridines; D015820:Cadherins / Q000378:metabolism*; D002227:Carbazoles / Q000494:pharmacology; D051177:Catenins; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D007211:Indoles / Q000494:pharmacology; D007700:Kinetics; D008698:Mesylates / Q000494:pharmacology; D010617:Phenanthridines / Q000494:pharmacology; D010750:Phosphoproteins / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D011651:Pulmonary Artery / Q000502:physiology*; D011758:Pyrroles / Q000494:pharmacology; D013917:Thrombin / Q000494:pharmacology*; D015534:Trans-Activators / Q000378:metabolism*; D051176:beta Catenin; D000095002:Delta Catenin; D000099152:Cadherin 5
D000470:Alkaloids; D015703:Antigens, CD; D053119:Benzophenanthridines; D015820:Cadherins; D002227:Carbazoles; D051177:Catenins; D015815:Cell Adhesion Molecules; D003598:Cytoskeletal Proteins; D004791:Enzyme Inhibitors; D007211:Indoles; D008698:Mesylates; D010617:Phenanthridines; D010750:Phosphoproteins; D011493:Protein Kinase C; D011758:Pyrroles; D013917:Thrombin; D015534:Trans-Activators; D051176:beta Catenin; D000095002:Delta Catenin; D000099152:Cadherin 5; C434605:5,21 - 12,17-dimetheneo-18H-dibenzo(i,o)pyrrolo(3,4-1)(1,8)diazacyclohexandecine-18,10(19H)dione,8((dimethylamino)methyl)-6,7,8,9,10,11-hexahydro,monomethanesulfonate; C495270:CTNNB1 protein, human; C081021:Go 6976; C016299:chelerythrine