[Preimplantation genetic diagnosis (PGD) of chromosomal aberrations using the fluorescence in situ hybridization method (FISH)--introduction to problems, sampling methods and examination techniques].
OBJECTIVE
Presentation of preimplantation genetic diagnosis and the set of laboratory processes like aspiration, preparation and evaluation of polar bodies, sperm cells and blastomeres using FISH method (fluorescent in situ hybridization) in ART.


METHODS
Review.


METHODS
Sanatorium REPROMEDA, Brno, Veterinary Research Institute, Brno.


METHODS
Overview of published data and own clinical experience with the cell aspiration methods, evaluated sample preparation and the proper chromosomes visualisation using FISH method.


CONCLUSIONS
The review brings an overview of conditions and methods including sample obtaining, FISH analysis preparation and implementation, processed during PGD.
Sanatorium REPROMEDA, Brno. kvesela@repromeda.cz
K
K
Veselá
G
G
Tauwinklová
P
P
Trávník
J
J
Veselý
S
S
Hlavácová
D
D
Zudová
O
O
Rezácová
R
R
Rybár
J
J
Rubes
D025063:Chromosome Disorders / Q000175:diagnosis*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D011247:Pregnancy; D019836:Preimplantation Diagnosis / Q000379:methods*