Pilot study of COBAS PCR and ligase chain reaction for detection of rectal infections due to Chlamydia trachomatis.
We tested rectal specimens from men who have sex with men for Chlamydia trachomatis by using COBAS PCR (Roche Diagnostics) and ligase chain reaction LCR (Abbott laboratories) and compared three PCR specimen-processing procedures. Chlamydiae were detected by one or more procedures in 22 of 186 specimens. All three PCR tests were positive for 17 specimens, all of which also tested positive by LCR.
Division of Infectious Diseases and the Center for AIDS & STD, University of Washington, and Public Health-Seattle and King County STD Program, Seattle, USA. golden@u.washington.edu
Matthew R
MR
Golden
Sabina G
SG
Astete
Rosa
R
Galvan
Aldo
A
Lucchetti
Jorge
J
Sanchez
Connie L
CL
Celum
William L H
WL
Whittington
Walter E
WE
Stamm
King K
KK
Holmes
Patricia A
PA
Totten
D002690:Chlamydia Infections / Q000175:diagnosis* / Q000382:microbiology / Q000635:transmission; D002692:Chlamydia trachomatis / Q000235:genetics; D018451:Homosexuality, Male; D006801:Humans; D022521:Ligase Chain Reaction / Q000379:methods*; D008297:Male; D010865:Pilot Projects; D016133:Polymerase Chain Reaction / Q000379:methods*; D012002:Rectal Diseases / Q000175:diagnosis* / Q000382:microbiology