The yggH gene of Escherichia coli encodes a tRNA (m7G46) methyltransferase.
We cloned, expressed, and purified the Escherichia coli YggH protein and show that it catalyzes the S-adenosyl-L-methionine-dependent formation of N(7)-methylguanosine at position 46 (m(7)G46) in tRNA. Additionally, we generated an E. coli strain with a disrupted yggH gene and show that the mutant strain lacks tRNA (m(7)G46) methyltransferase activity.
De Bie
Lara G S
LG
Laboratoire de Microbiologie, Université Libre de Bruxelles, B-1070 Brussels, Belgium.
Roovers
Martine
M
Oudjama
Yamina
Y
Wattiez
Ruddy
R
Tricot
Catherine
C
Stalon
Victor
V
Droogmans
Louis
L
Bujnicki
Janusz M
JM
D000595:Amino Acid Sequence; D001483:Base Sequence; D002455:Cell Division / Q000235:genetics; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism*; D006151:Guanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family; D012343:RNA, Transfer / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012436:S-Adenosylmethionine / Q000378:metabolism; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D012359:tRNA Methyltransferases / Q000235:genetics* / Q000378:metabolism*
D029968:Escherichia coli Proteins; D011994:Recombinant Proteins; D006151:Guanosine; C016578:7-methylguanosine; D012436:S-Adenosylmethionine; D012343:RNA, Transfer; C474650:YggH protein, E coli; D012359:tRNA Methyltransferases