Occurrence, horizontal transfer and degeneration of VDE intein family in Saccharomycete yeasts.
VDE is a homing endonuclease gene originally discovered as an intervening element in VMA1s of Saccharomyces cerevisiae. There have been two independent subfamilies of VDE, one from S. cerevisiae strain X2180-1A and the other from Saccharomyces sp. DH1-1A in the host VMA1 gene, and they share the identity of 96.3%. In order to search the occurrence, intra/interspecies transfer and molecular degeneration of VDE, complete sequences of VMA1 in 10 strains of S. cerevisiae, eight species of saccharomycete yeasts, Candida glabrata and Kluyveromyces lactis were determined. We found that six of 10 S. cerevisiae strains contain VDEs 99.7-100% identical to that of the strain X2180-1A, one has no VDE, whereas the other three harbour VDEs 100% identical to that of the strain DH1-1A. S. carlsbergensis has two VMA1s, one being 99.8% identical to that of the strain X2180-1A with VDE 100% identical to that of the strain DH1-1A and the other containing the same VMA1 in S. pastorianus with no VDE. This and other evidence indicates that intra/interspecies transmissions of VDEs have occurred among saccharomycete yeasts. Phylogenetic analyses of VMA1 and VDE suggest that the S. cerevisiae VDEs had branched earlier than other VDEs from an ancestral VDE and had invaded into the host loci as relatively late events. The two VDEs seemed to degenerate in individual host loci, retaining their splicing capacity intact. The degeneration of the endonuclease domains was distinct and, if compared, its apparent rate was much faster than that of the protein-splicing domains.
Okuda
Yoshihiro
Y
Department of Biosciences, Teikyo University of Science and Technology, Yatsusawa 2525, Uenohara-machi, Kitatsuru-gun, Yamanashi 409-0193, Japan.
Sasaki
Daisuke
D
Nogami
Satoru
S
Kaneko
Yoshinobu
Y
Ohya
Yoshikazu
Y
Anraku
Yasuhiro
Y
D019943:Amino Acid Substitution; D001483:Base Sequence; D041221:Candida glabrata / Q000201:enzymology / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D019143:Evolution, Molecular; D022761:Gene Transfer, Horizontal; D005800:Genes, Fungal; D007716:Kluyveromyces / Q000201:enzymology / Q000235:genetics; D008958:Models, Molecular; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D006180:Proton-Translocating ATPases / Q000737:chemistry / Q000235:genetics*; D012331:RNA, Fungal / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D012440:Saccharomyces / Q000201:enzymology / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics*; D004718:Saccharomycetales / Q000201:enzymology / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D004271:DNA, Fungal; D012331:RNA, Fungal; D012335:RNA, Ribosomal; D029701:Saccharomyces cerevisiae Proteins; D006180:Proton-Translocating ATPases; C081687:VMA1 protein, S cerevisiae