Schistosome N-glycans containing core alpha 3-fucose and core beta 2-xylose epitopes are strong inducers of Th2 responses in mice.
During murine schistosomiasis, egg-derived glycoconjugates play a key role in skewing the immune response towards a Th2 phenotype. Among the candidates responsible for this effect, complex-type N-glycans containing the core alpha 3-fucose and core beta 2-xylose determinants, two glycan epitopes found in some invertebrate- and plant-derived allergens, may be important. Here, we show that core alpha 3-fucose and core beta 2-xylose determinants are expressed in the different developmental stages of Schistosoma mansoni, particularly in the excretory-secretory systems of schistosomula and adult worms and in eggs deposited in the liver. Glycosyltransferase assays confirmed the presence of core alpha 3-fucosyltransferase and core beta 2-xylosyltransferase activities in egg extracts. Using a model of immunization with pulsed dendritic cells, we show that egg-derived glycoproteins containing the core alpha 3-fucose and core beta 2-xylose determinants generate a strong Th2-biased cellular response in mice and that the glycan moieties of this extract are important in this effect. During murine infection, these complex-type N-glycans induce a glycan-specific Th2 cellular response and elicit T-dependent anti-core alpha 3-fucose and anti-core beta 2-xylose IgG1 (a Th2-associated isotype), but not IgG2b (a Th1-associated isotype) Ab. Taken together, our results point out the importance of core fucosylated/xylosylated N-glycans in the Th2 immune response during murine schistosomiasis.
Inserm U547, IFR17, Institut Pasteur de Lille, Lille, France.
Christelle
C
Faveeuw
Thierry
T
Mallevaey
Katharina
K
Paschinger
Iain B H
IB
Wilson
Josette
J
Fontaine
Rosella
R
Mollicone
Rafael
R
Oriol
Friedrich
F
Altmann
Patrice
P
Lerouge
Monique
M
Capron
François
F
Trottein
D000818:Animals; D003713:Dendritic Cells / Q000276:immunology; D000939:Epitopes; D005260:Female; D005643:Fucose / Q000276:immunology*; D006001:Glycoconjugates / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology; D006735:Horseradish Peroxidase / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D010063:Ovum / Q000737:chemistry; D011134:Polysaccharides / Q000276:immunology*; D012550:Schistosoma mansoni / Q000737:chemistry; D012555:Schistosomiasis mansoni / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*; D014994:Xylose / Q000276:immunology*
D000939:Epitopes; D006001:Glycoconjugates; D006023:Glycoproteins; D011134:Polysaccharides; D005643:Fucose; D014994:Xylose; D006735:Horseradish Peroxidase