Regulation of BRCA1 transcription by specific single-stranded DNA binding factors.
Since the majority of high-grade breast cancers express reduced levels of BRCA1 mRNA, we investigated the factors regulating BRCA1 transcription. Factors with specific affinity for the previously identified positive regulatory region (PRR) in the BRCA1 promoter were purified from whole-cell extracts. Identified proteins included replication protein A and a series of related factors with affinity for the sense strand of PRR. A subset of the identified factors activated the BRCA1 promoter. Identification of these families of proteins regulating the BRCA1 promoter represents an important step in the comprehension of the mechanisms responsible for breast cancer development.
Kimmel Cancer Center, Philadelphia, Pennsylvania 19107, USA.
Sanjay
S
Thakur
Tatsuya
T
Nakamura
George
G
Calin
Andrea
A
Russo
Joseph F
JF
Tamburrino
Masayoshi
M
Shimizu
Gustavo
G
Baldassarre
Sabrina
S
Battista
Alfredo
A
Fusco
Richard P
RP
Wassell
Garret
G
Dubois
Hansjuerg
H
Alder
Carlo M
CM
Croce
D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D019398:Genes, BRCA1; D006801:Humans; D008969:Molecular Sequence Data; D017354:Point Mutation; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins