Antispasmodic effect of three fractions of hydroalcoholic extract of Pycnocycla spinosa.
Hydroalcoholic extract of Pycnocycla spinosa was shown to have spasmolytic action in vitro and antidiarroheal effect in vivo. The hydroalcoholic extract of P. spinosa is composed of alkaloid-, flavonoid- and saponin-rich components. These components were separated by fractional separation technique. The pharmacological objective of this study was to look for relaxant effect of these components of hydroalcoholic extract of P. spinosa on rat isolated ileum contractions. The alkaloid-, flavonoid- and saponin-rich fractions of P. spinosa inhibited the response to 80 mM KCl in a concentration-dependent manner. The alkaloid-rich fraction was the most and saponin-rich fraction the least effective relaxant of ileum contractions. The alkaloid-rich fraction also inhibited the response to acetylcholine (ACh) and 5-hydroxytryptamine (5-HT). From this study, it can be concluded that the antispasmodic action of hydroalcoholic extract of P. spinosa could be due to components in the alkaloid-rich fraction and to a lesser extent, to its flavonoid-rich fraction.
Department of Pharmacology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Iran. hsadraei@yahoo.com
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D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D019661:Apiaceae; D004347:Drug Interactions; D007082:Ileum / Q000187:drug effects; D008297:Male; D009126:Muscle Relaxation / Q000187:drug effects*; D010276:Parasympatholytics / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D000470:Alkaloids; D010276:Parasympatholytics; D010936:Plant Extracts