Plasmid profile in oral Fusobacterium nucleatum from humans and Cebus apella monkeys.
Fusobacterium nucleatum is a strict anaerobe and is indigenous of the human oral cavity. This organism is commonly recovered from different monomicrobial and mixed infections in humans and animals. In this study, the plasmid profile, the plasmid stability and the penicillin-resistance association in oral F. nucleatum isolated from periodontal patients, healthy subjects and Cebus apella monkeys were evaluated. Forty-five F. nucleatum strains from patients, 38 from healthy subjects and seven from C. apella were identified and analyzed. Plasmid extraction was performed in all the isolated strains. These elements were found in 26.7% strains from patients and one strain from C. apella. Strains from healthy subjects did not show any plasmid. Most of strains showed two plasmid bands ranging from 4 to 16 Kb, but digestions with endonucleases showed that they belonged to a single plasmid. The plasmid profile was similar and stable in human and monkey strains. Also, plasmids were classified into three groups according to size. Two strains were positive to beta-lactamase production and no plasmid DNA-hybridization with a beta-lactamase gene probe was observed, suggesting a chromosomal resistance.
Paula
Marcia O
MO
School of Biology, Centro Universitário Adventista de São Paulo, São Paulo, SP, Brazil.
Gaetti-Jardim Júnior
Elerson
E
Avila-Campos
Mario J
MJ
D000328:Adult; D000818:Animals; D015373:Bacterial Typing Techniques; D015139:Blotting, Southern; D002428:Cebus / Q000382:microbiology; D004269:DNA, Bacterial; D004587:Electrophoresis, Agar Gel; D016967:Fusobacterium nucleatum / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D006801:Humans; D009055:Mouth / Q000382:microbiology*; D010403:Penicillin Resistance / Q000235:genetics*; D010518:Periodontitis / Q000382:microbiology*; D010957:Plasmids / Q000235:genetics* / Q000302:isolation & purification; D001618:beta-Lactamases / Q000096:biosynthesis
D004269:DNA, Bacterial; D001618:beta-Lactamases