Specific detection of organotin compounds with a recombinant luminescent bacteria.
Organotin compounds are widely used as biocides in marine and terrestrial environments. Several currently used techniques allow either the measurement of the chemicals or their effects on living organisms. Our current research focuses on the development of a complementary method based on a bacterial bioluminescence-based bioassay for the specific detection of organotin compounds. The performance of the bioassay was assessed. The Escherichia coli bacterial strain used in this study is specific for TBT and DBT (with Cl, Br or I as the halogen group) with the central tin atom important for light production. The assay is conducted after overnight culture of the bacterial strain, followed by 60 min of contact time with the organotin compound for significant light production. The detection limits were found to be 0.08 microM for TBT (26 microgl(-1)) and 0.0001 microM for DBT (0.03 microgl(-1)) with a linear range of one logarithm. The repeatability of the bioassay is 8% and the reproducibility for TBT and DBT was approximately 14%. Lyophilization of the strains did not significantly modify the detection limit as well as the range of detection. Applications of the bioassay to environmental samples are discussed.
Durand
Marie José
MJ
Département Génie Biologique, Laboratoire de Microbiologie, Université de Nantes, IUT, 18 Bd G. Defferre, 85000, La Roche sur Yon, France.
Thouand
Gérald
G
Dancheva-Ivanova
Tania
T
Vachon
Patricia
P
DuBow
Michael
M
D001681:Biological Assay / Q000379:methods; D004784:Environmental Monitoring / Q000379:methods*; D004926:Escherichia coli / Q000502:physiology*; D008163:Luminescent Measurements; D009947:Organotin Compounds / Q000009:adverse effects* / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014220:Trialkyltin Compounds / Q000009:adverse effects* / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000009:adverse effects* / Q000032:analysis*
D009947:Organotin Compounds; D014220:Trialkyltin Compounds; D014874:Water Pollutants, Chemical; C056189:di-n-butyltin; C011559:tributyltin