CpG-motifs enhance initial and sustained primary tetanus-specific antibody secreting cell responses in spleen and bone marrow, but are more effective in adult than in neonatal mice.
Unmethylated CpG oligonucleotides (CpG-ODN) increase adult and neonatal primary antibody responses to T-dependent antigens, at yet unidentified stages of antigen-specific B cell differentiation. In adult mice, a single dose of CpG-ODN adjuvanted tetanus toxoid (TT) vaccine markedly enhanced and prolonged splenic TT-specific antibody-secreting-cell (ASC) responses and significantly increased the size of the bone marrow (BM) ASC pool. Surprisingly, this was not associated with changes of germinal center (GC) numbers, size, apoptosis or function. In 1-week-old mice, CpG-ODN also enhanced TT-specific splenic ASC responses, but failed to correct limitations of the GC reaction and of the development of the BM ASC pool.
Department of Pathology and Pediatrics, World Health Organization Collaborating Center for Vaccinology and Neonatal Immunology, CMU, Rue Michel Servet 1, 1211 Geneva 4, Switzerland.
Maria
M
Pihlgren
Chantal
C
Tougne
Nadine
N
Schallert
Paola
P
Bozzotti
Paul-Henri
PH
Lambert
Claire-Anne
CA
Siegrist
D000375:Aging / Q000276:immunology; D000818:Animals; D000831:Animals, Newborn; D000907:Antibodies, Bacterial / Q000276:immunology*; D000915:Antibody Affinity; D001853:Bone Marrow / Q000276:immunology; D015226:Dinucleoside Phosphates / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D007074:Immunoglobulin G / Q000097:blood / Q000145:classification; D051379:Mice; D008807:Mice, Inbred BALB C; D009838:Oligodeoxyribonucleotides / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013742:Tetanus / Q000097:blood / Q000276:immunology*; D013745:Tetanus Toxoid / Q000276:immunology*
D000907:Antibodies, Bacterial; D015226:Dinucleoside Phosphates; D007074:Immunoglobulin G; D009838:Oligodeoxyribonucleotides; D013745:Tetanus Toxoid; C015772:CpG dinucleotide