Phagocytosis of bovine blood and milk polymorphonuclear leukocytes after ozone gas administration in vitro.
To determine the effects of ozone on the phagocytosis of bovine polymorphonuclear leukocytes (PMNs), ozone gas was administered in vitro on the blood and milk of healthy lactating cows, cows with acute mastitis, and cows with milk fever. In the blood of healthy dairy cattle, although there was no significant effect of ozone gas on the viability of the leukocytes, phagocytosis of PMNs significantly decreased. In contrast, ozone gas administration in vitro significantly increased phagocytosis of PMNs from the blood of cows with acute mastitis and milk fever, and from mastitic milk. These findings showed that ozone administration in vitro has positive and negative effects on bovine PMN phagocytosis, depending on the health status of the animal.
Department of Veterinary Clinical Science, The United Graduate School of Veterinary Sciences, Gifu University, Yanagido, Japan.
Rio John T
RJ
Ducusin
Masakazu
M
Nishimura
Takao
T
Sarashina
Yuji
Y
Uzuka
Shigeyuki
S
Tanabe
Masayuki
M
Otani
D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000276:immunology; D002478:Cells, Cultured; D005260:Female; D007612:Kanamycin / Q000627:therapeutic use; D008414:Mastitis, Bovine / Q000097:blood / Q000188:drug therapy / Q000276:immunology; D008892:Milk / Q000166:cytology*; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology; D010083:Oxidants, Photochemical / Q000494:pharmacology*; D010126:Ozone / Q000494:pharmacology*; D010319:Parturient Paresis / Q000097:blood / Q000276:immunology; D010406:Penicillins / Q000627:therapeutic use; D010587:Phagocytosis / Q000187:drug effects*; D011247:Pregnancy; D013307:Streptomycin / Q000627:therapeutic use
D000900:Anti-Bacterial Agents; D010083:Oxidants, Photochemical; D010406:Penicillins; D007612:Kanamycin; D010126:Ozone; D013307:Streptomycin