Reductive dehalogenation of chlorobenzene congeners in cell extracts of Dehalococcoides sp. strain CBDB1.
Enzymatic reductive dehalogenation of tri-, tetra-, penta-, and hexachlorobenzenes was demonstrated in cell extracts with low protein concentration (0.5 to 1 micro g of protein/ml) derived from the chlorobenzene-respiring anaerobe Dehalococcoides sp. strain CBDB1. 1,2,3-trichlorobenzene dehalogenase activity was associated with the membrane fraction. Light-reversible inhibition by alkyl iodides indicated the presence of a corrinoid cofactor.
Hölscher
Tina
T
Fachgebiet Technische Biochemie, Institut für Biotechnologie, Technische Universität Berlin, Seestrasse 13, D-13353 Berlin, Germany. tina.hoelscher@tu-berlin.de
Görisch
Helmut
H
Adrian
Lorenz
L
D000332:Aerobiosis; D000693:Anaerobiosis; D001419:Bacteria / Q000378:metabolism*; D002722:Chlorobenzenes / Q000737:chemistry / Q000378:metabolism*; D004785:Environmental Pollutants / Q000378:metabolism; D006867:Hydrolases / Q000378:metabolism; D010084:Oxidation-Reduction; D013347:Subcellular Fractions / Q000378:metabolism
D002722:Chlorobenzenes; D004785:Environmental Pollutants; D006867:Hydrolases