Activation of defense-related gene expression and systemic acquired resistance in cucumber mosaic virus-infected tobacco plants expressing the mammalian 2'5'oligoadenylate system.
Tobacco plants expressing the mammalian 2'5'oligoadenylate system (2-5A system) exhibit resistance to cucumber mosaic virus (CMV). Here, to characterize the molecular aspect of the resistance to CMV in 2-5A system-expressing tobaccos, the activation of defense-related genes and systemic acquired resistance (SAR) as the markers for the hypersensitive resistance (HR), were elucidated. Northern hybridization analysis indicated that the expression of four pathogenesis-related (PR) protein genes and five HR-related genes were induced in CMV-infected tobaccos expressing 2-5A system. Furthermore, the induction of SAR against Pseudomonas syringae pv. tabaci as second challenge, was observed on CMV-inoculated tobaccos expressing 2-5A system. These results suggested that the resistance to CMV in tobacco expressing 2-5A system is associated with the establishment of an HR-like response.
Honda
A
A
Department of Life Science, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Takahashi
H
H
Toguri
T
T
Ogawa
T
T
Hase
S
S
Ikegami
M
M
Ehara
Y
Y
D000227:Adenine Nucleotides / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D017799:Cucumovirus / Q000502:physiology*; D004198:Disease Susceptibility; D018506:Gene Expression Regulation, Plant; D009843:Oligoribonucleotides / Q000235:genetics* / Q000378:metabolism*; D010935:Plant Diseases / Q000821:virology; D018515:Plant Leaves / Q000235:genetics / Q000821:virology; D030821:Plants, Genetically Modified; D014026:Nicotiana / Q000235:genetics* / Q000821:virology*
D000227:Adenine Nucleotides; D009843:Oligoribonucleotides; C023505:2',5'-oligoadenylate