Derivation of oocytes from mouse embryonic stem cells.
Continuation of mammalian species requires the formation and development of the sexually dimorphic germ cells. Cultured embryonic stem cells are generally considered pluripotent rather than totipotent because of the failure to detect germline cells under differentiating conditions. Here we show that mouse embryonic stem cells in culture can develop into oogonia that enter meiosis, recruit adjacent cells to form follicle-like structures, and later develop into blastocysts. Oogenesis in culture should contribute to various areas, including nuclear transfer and manipulation of the germ line, and advance studies on fertility treatment and germ and somatic cell interaction and differentiation.
Germline Development Group, Center for Animal Transgenesis and Germ Cell Research, School of Veterinary Medicine, University of Pennsylvania, New Bolton Center, 382 West Street Road, Kennett Square, PA 19348, USA.
Karin
K
Hübner
Guy
G
Fuhrmann
Lane K
LK
Christenson
James
J
Kehler
Rolland
R
Reinbold
Rabindranath
R
De La Fuente
Jennifer
J
Wood
Jerome F
JF
Strauss
Michele
M
Boiani
Hans R
HR
Schöler
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D001755:Blastocyst / Q000166:cytology / Q000502:physiology*; D002448:Cell Adhesion; D002449:Cell Aggregation; D002454:Cell Differentiation; D019070:Cell Lineage; D002469:Cell Separation; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000235:genetics; D004622:Embryo, Mammalian / Q000166:cytology*; D004958:Estradiol / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D017930:Genes, Reporter; D008540:Meiosis; D051379:Mice; D008822:Mice, Transgenic; D050814:Octamer Transcription Factor-3; D009865:Oocytes / Q000166:cytology / Q000502:physiology*; D009866:Oogenesis; D006080:Ovarian Follicle / Q000166:cytology / Q000502:physiology; D011993:Recombinant Fusion Proteins; D039901:Totipotent Stem Cells / Q000502:physiology*; D014157:Transcription Factors; D014162:Transfection
D015415:Biomarkers; D004268:DNA-Binding Proteins; D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D004958:Estradiol