Members of the Arabidopsis WRKY group III transcription factors are part of different plant defense signaling pathways.
WRKY proteins are a large group of transcription factors restricted to the plant kingdom. In Arabidopsis thaliana, the gene family consists of 74 members. Here, we analyzed the expression of all 13 members of one main WRKY subgroup and found that the majority are responsive both to pathogen infection and to salicylic acid. Temporal expression studies during compatible, incompatible, and nonhost interactions and employing plant defense-signaling mutants allowed us to define four distinct WRKY subsets responding to different signaling queues along defense pathways. These subsets did not reflect phylogenetic relationships. Promoter studies of one member, AtWRKY54, using a reporter gene construct in transgenic Arabidopsis plants, revealed that regulatory regions mediating pathogen and SA inducibility are clearly separable. In an AtWRKY54 knockout line, resistance to Peronospora parasitica was not compromised, but the transient expression kinetics of several WRKY genes was affected, suggesting both the existence of functional redundancy and intense cross-talk between signaling networks.
Kalde
Monika
M
Max-Planck-lnstitut für Züchtungsforschung, Abteilung Molekulare Phytopathologie, Carl-von-Linné Weg 10, D-50829 Köln, Germany.
Barth
Meike
M
Somssich
Imre E
IE
Lippok
Bernadette
B
D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D017353:Gene Deletion; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D007113:Immunity, Innate; D009868:Oomycetes / Q000472:pathogenicity*; D010802:Phylogeny; D010935:Plant Diseases; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D015398:Signal Transduction / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
D029681:Arabidopsis Proteins; D014157:Transcription Factors