The polysaccharide fraction AIP1 from Artemisia iwayomogi suppresses apoptotic death of the mouse spleen cells in culture.
A polysaccharide fraction, AIP1, purified from Artemisia iwayomogi was shown to have immunomodulating and anti-tumor activities in mice. In order to determine how the AIP1 fraction exhibits the immunomodulating activity, the effect of the fraction on the apoptosis of mouse spleen cells was investigated. Treatment of the mouse spleen cells with the AIP1 fraction resulted in the suppression of apoptotic death and an extension of cell survival in culture, indicating that the fraction might modulate the death of spleen cells. Treatment of the mice with the AIP1 fraction in vivo also resulted in less apoptosis of the spleen cells, which indicates the physiological relevance of the anti-apoptosis effect of the fraction in vitro. A mouse gene array was used to determine the profile of the gene expression change showing a pattern of up- and down-regulated genes by the AIP1 treatment. This study provides preliminary information regarding the immunomodulatory mechanism of the AIP1 fraction.
Hwang
Jung Suk
JS
Department of Microbiology, School of Biotechnology & Biomedical Science, Inje University, Gimhae, Gyungnam 621-749, Korea.
Chung
Hye Kyung
HK
Bae
Eun Kyong
EK
Lee
Ah Young
AY
Ji
Hee Jung
HJ
Park
Dong Woon
DW
Jung
Hwa Jin
HJ
Cho
Chung Won
CW
Choi
Hyun Ju
HJ
Lee
Dong Seok
DS
Lee
Kang Ro
KR
Youn
Hyun Joo
HJ
D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics*; D019306:Artemisia; D001854:Bone Marrow Cells / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects*; D005260:Female; D015870:Gene Expression / Q000187:drug effects* / Q000235:genetics*; D017737:Macrophages, Peritoneal / Q000187:drug effects; D051379:Mice; D010936:Plant Extracts; D018515:Plant Leaves; D011134:Polysaccharides / Q000235:genetics* / Q000276:immunology / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology* / Q000187:drug effects*
D010936:Plant Extracts; D011134:Polysaccharides