MAP kinase-dependent induction of clock gene expression by alpha 1-adrenergic receptor activation.
While peripheral oscillators can be reset by humoral factors such as glucocorticoid hormones, indirect neural communications involving sympathetic and parasympathetic neurons from the suprachiasmatic nucleus to various peripheral tissues suggest that autonomic nerve innervations also function in the resetting and synchronization of peripheral tissues. To study the role of sympathetic adrenergic signaling on clock gene expression, we constructed NIH3T3 cells that stably expressed each of three alpha(1)-adrenergic receptor subtypes (alpha(1A), alpha(1B) and alpha(1D)). We found that noradrenaline transiently induced the expression of mPer1, mPer2, and mE4bp4 1-2 h after alpha(1)-receptor activation. The extent and time course of clock gene mRNA induction by noradrenaline or the alpha(1)-receptor agonist phenylephrine (PE) was similar to that seen by 50% horse serum shock. Clock gene mRNA induction by PE was inhibited by U0126, a MEK inhibitor, suggesting involvement of the mitogen-activated protein kinase signaling pathway. We also found that both mPer1 and mPer2 mRNAs were induced in the mouse liver 60 min after PE injection. These results suggest that although humoral factors are important for entrainment of the peripheral clock, the autonomic nervous system may also be involved in the process.
Department of Pharmacology and Brain Science, School of Human Sciences, Waseda University, Tokorozawa, Saitama 359-1192, Japan.
Masashi
M
Akiyama
Yoichi
Y
Minami
Kouji
K
Kuriyama
Shigenobu
S
Shibata
D016475:3T3 Cells; D000316:Adrenergic alpha-Agonists / Q000494:pharmacology; D000818:Animals; D056926:CLOCK Proteins; D018797:Cell Cycle Proteins; D005786:Gene Expression Regulation; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D020935:MAP Kinase Signaling System; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000502:physiology*; D009638:Norepinephrine / Q000494:pharmacology; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000235:genetics; D056950:Period Circadian Proteins; D010656:Phenylephrine / Q000494:pharmacology; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis / Q000235:genetics; D018340:Receptors, Adrenergic, alpha-1 / Q000378:metabolism*; D015534:Trans-Activators / Q000096:biosynthesis* / Q000235:genetics; D014157:Transcription Factors
C547713:ADRA1A protein, human; C547714:Adra1a protein, mouse; D000316:Adrenergic alpha-Agonists; D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; C539022:PER1 protein, human; C539023:Per1 protein, mouse; C513312:Per2 protein, mouse; D056950:Period Circadian Proteins; D016760:Proto-Oncogene Proteins c-fos; D018340:Receptors, Adrenergic, alpha-1; D015534:Trans-Activators; D014157:Transcription Factors; D010656:Phenylephrine; D056926:CLOCK Proteins; C538943:CLOCK protein, human; C538944:Clock protein, mouse; D020928:Mitogen-Activated Protein Kinases; D009638:Norepinephrine