Current and future applications of SAGE to cardiovascular medicine.
The recently sequenced mammalian genomes represent unprecedented resources for advancing our understanding of human diseases. Characterizing gene expression is an important step in translating genomic sequences into clinically useful information. Currently, gene expression studies are revolutionizing the approaches taken to address both basic science and clinical questions. Two major methods have emerged for the global examination of the transcriptome: microarrays and Serial Analysis of Gene Expression (SAGE). The SAGE technique comprehensively maps gene transcription by using the genomic database, yet it remains relatively underutilized for studying cardiovascular biology. This review describes current cardiovascular studies using the SAGE technique and outlines some potential strategies for employing this powerful tool to further our understanding of the cardiovascular system in health and in disease.
Patino
Willmar D
WD
Cardiovascular Branch, National Heart, Lung, and Blood Institute, The National Institutes of Health, Bethesda, MD 20892, USA.
Mian
Omar Y
OY
Shizukuda
Yukitaka
Y
Hwang
Paul M
PM
D000818:Animals; D002318:Cardiovascular Diseases / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics; D020869:Gene Expression Profiling / Q000639:trends; D005821:Genetic Techniques / Q000639:trends; D006801:Humans