[Isolation and purification of Lycium barbarum polysaccharides and its antifatigue effect].
A purified component of lycium barbarum polysaccharide (LBP-X) was isolated from lycium barbarum L. by DEAE ion-exchange cellulose and sephacryl gel chromatography. LBP-X was tested on five different doses (5, 10, 20, 50 and 100 mg.kg-1.d-1) in mice. The results showed that LBP-X induced a remarkable adaptability to exercise load, enhanced resistance and accelerated elimination of fatigue. LBP-X could enhance the storage of muscle and liver glycogen, increase the activity of LDH before and after swimming, decrease the increase of blood urea nitrogen (BUN) after strenuous exercise, and accelerate the clearance of BUN after exercise. The dosage of LBP-X 10 mg.kg-1.d-1 was the best amount among the five tested doses.
Department of Hygiene, Hubei Medical University, Wuhan 430071, China.
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Luo
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Yan
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Zhang
D000818:Animals; D001806:Blood Urea Nitrogen; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D005221:Fatigue / Q000378:metabolism*; D005638:Fruit / Q000737:chemistry; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D032305:Lycium / Q000737:chemistry*; D051379:Mice; D005082:Physical Exertion; D010946:Plants, Medicinal / Q000737:chemistry*; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology*
D004365:Drugs, Chinese Herbal; D011134:Polysaccharides; D007770:L-Lactate Dehydrogenase