In vitro generation of T lymphocytes from embryonic stem cell-derived prehematopoietic progenitors.
Embryonic stem (ES) cells can differentiate into most blood cells in vitro, providing a powerful model system to study hematopoiesis. However, ES cell-derived T lymphocytes have not been generated in vitro, and it was unresolved whether such potential is absent or merely difficult to isolate. Because the latter case might result from rapid commitment to non-T-cell fates, we isolated ES cell-derived prehematopoietic precursors for reconstitution of fetal thymic organ cultures. We found a transient Flk1+CD45- subset of these precursors generated T lymphocytes in vitro, and the use of reaggregate thymic organ cultures greatly enhanced reconstitution frequency. These findings reveal that ES cells can exhibit in vitro T-cell potential, but this is restricted to early stages of ES cell differentiation. Moreover, the results support the notion that the thymic microenvironment can induce T-cell differentiation from a subset of prehematopoietic progenitors and suggest deficient migration into intact thymi hindered previous attempts to generate T cells in vitro from ES cell-derived progenitors. These findings demonstrate that a defined subset of ES cells has the potential to generate T cells in vitro and could contribute to greater understanding of the molecular events of hematopoietic induction and T-cell lineage commitment.
Department of Immunology, University of Toronto, Sunnybrook & Women's College Health Sciences Centre, 2075 Bayview Ave, Rm A-331, Toronto, ON, M4N 3M5, Canada. jc.zuniga.pflucker@utoronto.ca
Renee F
RF
de Pooter
Sarah K
SK
Cho
James R
JR
Carlyle
Juan Carlos
JC
Zúñiga-Pflücker
D000818:Animals; D002449:Cell Aggregation / Q000276:immunology; D002454:Cell Differentiation / Q000276:immunology; D019070:Cell Lineage / Q000276:immunology; D018920:Coculture Techniques; D005260:Female; D005333:Fetus; D006412:Hematopoietic Stem Cells / Q000737:chemistry / Q000166:cytology*; D017493:Leukocyte Common Antigens / Q000032:analysis; D051379:Mice; D020297:Mice, Congenic; D008810:Mice, Inbred C57BL; D009924:Organ Culture Techniques; D011247:Pregnancy; D013601:T-Lymphocytes / Q000737:chemistry / Q000166:cytology*; D013950:Thymus Gland / Q000166:cytology* / Q000196:embryology; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000032:analysis
D040301:Vascular Endothelial Growth Factor Receptor-2; D017493:Leukocyte Common Antigens