Role of nitric oxide in the response of Saccharomyces cerevisiae cells to heat shock and high hydrostatic pressure.
Nitric oxide (NO) is a simple and unique molecule that has diverse functions in organisms, including intracellular and intercellular messenger. The influence of NO on cell growth of Saccharomyces cerevisiae and as a signal molecule in stress response was evaluated. Respiring cells were more sensitive to an increase in intracellular NO concentration than fermentatively growing cells. Low levels of NO demonstrated a cytoprotective effect during stress from heat-shock or high hydrostatic pressure. Induction of NO synthase was isoform-specific and dependent on the metabolic state of the cells and the stress response pathway. These results support the hypothesis that an increase in intracellular NO concentration leads to stress protection.
Domitrovic
Tatiana
T
Dept. C. Fisiológicas/CBM/UFES Av. Marechal Campos, 1468 Marui;pe 29040-090, ES, Vitória, Brazil.
Palhano
Fernando L
FL
Barja-Fidalgo
Christina
C
DeFreitas
Martha
M
Orlando
Marcos T D
MT
Fernandes
Patricia M B
PM
D015153:Blotting, Western; D005656:Fungal Proteins / Q000378:metabolism; D018869:Heat-Shock Response / Q000502:physiology*; D006874:Hydrostatic Pressure; D007527:Isoenzymes / Q000378:metabolism; D009569:Nitric Oxide / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000378:metabolism; D009599:Nitroprusside / Q000494:pharmacology; D026423:S-Nitroso-N-Acetylpenicillamine / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000254:growth & development / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology
D005656:Fungal Proteins; D007527:Isoenzymes; D020030:Nitric Oxide Donors; D009599:Nitroprusside; D009569:Nitric Oxide; D026423:S-Nitroso-N-Acetylpenicillamine; D019001:Nitric Oxide Synthase