Maximization of recombinant Helicobacter pylori neutrophil activating protein production in Escherichia coli: improvement of a chemically defined medium using response surface methodology.
Medium optimization for the production of constitutive recombinant Helicobacter pylori neutrophil activating protein (NAP) in Escherichia coli was investigated by using response surface methodology. Carbon to nitrogen ratio, concentrations of sodium polyphosphate and magnesium sulfate were considered as independent variables. The optimized medium was a chemically defined medium with a carbon to nitrogen ratio of 14.4 and with concentrations of sodium polyphosphate and magnesium sulfate about 7.1 g l(-1) and 3.04 g l(-1) respectively. The maximum recombinant NAP production level (1184.6 mg l(-1)) was 29.96% higher than that in control medium.
Niccolai
Andrea
A
Microbial Fermentation Department, Process Development Division, Chiron S.r.L., Siena, Italy.
Fontani
Silvia
S
Kapat
Arnab
A
Olivieri
Roberto
R
D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D002244:Carbon / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media / Q000737:chemistry*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D016480:Helicobacter pylori / Q000235:genetics* / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry*
D001426:Bacterial Proteins; D003470:Culture Media; D011994:Recombinant Proteins; D013501:Surface-Active Agents; C092412:neutrophil-activating protein A, Helicobacter pylori; D002244:Carbon; D009584:Nitrogen