Expression of rotavirus capsid protein VP6 in transgenic potato and its oral immunogenicity in mice.
Murine rotavirus gene six encoding the 41 kDa group specific capsid structural protein VP6 was stably inserted into the Solanum tuberosum genome by Agrobacterium tumefaciens mediated transformation. The molecular mass of plant synthesized VP6 capsid protein determined by immunoblot was similar to the size of both purified virus VP6 monomeric peptides and partially assembled virus-like particles. The amount of VP6 protein synthesized in transgenic potato leaf and tuber was determined by enzyme-linked immunosorbent assay to be approximately 0.01% of total soluble protein. Oral immunization of CD-1 mice with transformed potato tuber tissues containing VP6 capsid protein generated measurable titers of both anti-VP6 serum IgG and intestinal IgA antibodies. The presence of detectable humoral and intestinal antibody responses against the rotavirus capsid protein following mucosal immunization provides an optimistic basis for the development of edible plant vaccines against enteric viral pathogens.
Biochemistry Department, Center for Molecular Biology and Gene Therapy, School of Medicine, Loma Linda University, Loma Linda, CA 92350, USA.
Jie
J
Yu
William
W
Langridge
D000284:Administration, Oral; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D015139:Blotting, Southern; D036022:Capsid Proteins / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D030821:Plants, Genetically Modified / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000235:genetics* / Q000276:immunology; D011198:Solanum tuberosum / Q000235:genetics*
D000914:Antibodies, Viral; D036022:Capsid Proteins; D018744:DNA, Plant; D018749:RNA, Plant