Transactivation of human cdc2 promoter by adenovirus E1A.
Expression of the adenovirus oncoprotein E1A 12S induces the heterotrimeric transcription factor, NF-Y. NF-Y binds to the two CCAAT motifs upstream of the transcriptional start site of the human cdc2 promoter and is required for activation of the promoter by E1A 12S in cycling cells. The observations that a number of eukaryotic cell cycle regulatory genes also contain the CCAAT motifs and NF-Y binds to them support the notion that E1A 12S could play an important role in deregulated expression of these genes through activation of NF-Y gene in cycling cells.
Department of Microbiology and Immunology, Georgetown University Medical Center, 3900 Reservoir Road, Washington DC, WA 20057, USA. rp55@georgetown.edu
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R
Padmanabhan
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Tanimoto
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Y
Sasaguri
D017500:Adenovirus E1A Proteins / Q000494:pharmacology*; D000260:Adenoviruses, Human / Q000737:chemistry; D016203:CDC2 Protein Kinase / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D011401:Promoter Regions, Genetic / Q000187:drug effects*; D014157:Transcription Factors / Q000378:metabolism; D015533:Transcriptional Activation / Q000187:drug effects*
D017500:Adenovirus E1A Proteins; D014157:Transcription Factors; D016203:CDC2 Protein Kinase