Prolyl endopeptidase inhibitory peptides in wine.
Two peptides that inhibit prolyl endopeptidase were isolated from a red wine made from Cabernet Sauvignon grapes. Their amino acid sequences and IC50 values were Val-Glu-Ile-Pro-Glu (17.0 microM) and Tyr-Pro-Ile-Pro-Phe (87.8 microM). The peptides also suppressed the degradation levels of the bioactive peptides vasopressin, substance P, and neurotensin fragments 8-13, which are involved in memory and neural communication.
Yanai
Takaaki
T
Mercian Corporation, Wine & Spirits Research Institute, 9-1 Johnan 4-chome, Fujisawa 251-0057, Japan. yanai-t@mercian.co.jp
Suzuki
Yumiko
Y
Sato
Michikatsu
M
D000595:Amino Acid Sequence; D020128:Inhibitory Concentration 50; D009496:Neurotensin / Q000037:antagonists & inhibitors; D010446:Peptide Fragments / Q000037:antagonists & inhibitors; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D000085822:Prolyl Oligopeptidases; D012697:Serine Endopeptidases / Q000378:metabolism*; D015842:Serine Proteinase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D013373:Substance P / Q000037:antagonists & inhibitors; D014667:Vasopressins / Q000037:antagonists & inhibitors; D027843:Vitis / Q000737:chemistry / Q000145:classification; D014920:Wine / Q000032:analysis*
D010446:Peptide Fragments; D010455:Peptides; D015842:Serine Proteinase Inhibitors; D014667:Vasopressins; D013373:Substance P; D009496:Neurotensin; C054381:neurotensin (8-13); D012697:Serine Endopeptidases; D000085822:Prolyl Oligopeptidases