The Corynebacterium glutamicum genome: features and impacts on biotechnological processes.
Corynebacterium glutamicum has played a principal role in the progress of the amino acid fermentation industry. The complete genome sequence of the representative wild-type strain of C. glutamicum, ATCC 13032, has been determined and analyzed to improve our understanding of the molecular biology and physiology of this organism, and to advance the development of more efficient production strains. Genome annotation has helped in elucidation of the gene repertoire defining a desired pathway, which is accelerating pathway engineering. Post genome technologies such as DNA arrays and proteomics are currently undergoing rapid development in C. glutamicum. Such progress has already exposed new regulatory networks and functions that had so far been unidentified in this microbe. The next goal of these studies is to integrate the fruits of genomics into strain development technology. A novel methodology that merges genomics with classical strain improvement has been developed and applied for the reconstruction of classically derived production strains. How can traditional fermentation benefit from the C. glutamicum genomic data? The path from genomics to biotechnological processes is presented.
Ikeda
M
M
Tokyo Research Laboratories, Kyowa Hakko Kogyo, Asahi-machi, Machida, 194-8533, Tokyo, Japan. m.ikeda@kyowa.co.jp
Nakagawa
S
S
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000378:metabolism; D001482:Base Composition; D001709:Biotechnology / Q000639:trends; D002874:Chromosome Mapping; D003352:Corynebacterium / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005285:Fermentation; D016680:Genome, Bacterial; D023281:Genomics; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D040901:Proteomics; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D000596:Amino Acids; D004269:DNA, Bacterial; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases