Targeted gene disruption of the neuronal calcium sensor 1 homologue in rice blast fungus, Magnaporthe grisea.
We isolated a neuronal calcium sensor 1/frequenin-like gene, Mg-NCS-1, from Magnaporthe grisea and evaluated the phenotypes of null-mutants of the gene. The putative Mg-NCS-1 protein showed high similarity to the other NCS-1 proteins. The null-mutants had normal growth and pathogenicity similar to the parental strain, but their growth was suppressed in high concentrations of Ca2+ or acidic conditions.
Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan.
Ken-ichiro
K
Saitoh
Tsutomu
T
Arie
Tohru
T
Teraoka
Isamu
I
Yamaguchi
Takashi
T
Kamakura
D000595:Amino Acid Sequence; D002118:Calcium / Q000737:chemistry / Q000494:pharmacology; D020013:Calcium Signaling; D002135:Calcium-Binding Proteins / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D020082:Magnaporthe / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D008969:Molecular Sequence Data; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000235:genetics; D051580:Neuronal Calcium-Sensor Proteins; D009479:Neuropeptides / Q000235:genetics*; D010641:Phenotype; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014170:Transformation, Genetic
D002135:Calcium-Binding Proteins; D005656:Fungal Proteins; D009419:Nerve Tissue Proteins; D051580:Neuronal Calcium-Sensor Proteins; D009479:Neuropeptides; C101464:frequenin calcium sensor proteins; D002118:Calcium