Protective effects of polygodial on gastric mucosal lesions induced by necrotizing agents in rats and the possible mechanisms of action.
The effects of polygodial isolated from the leaves of Tasmannia lanceolata on necrotizing agents-induced gastric lesions in rats were compared with capsaicin. Polygodial markedly inhibited the gastric mucosal lesions induced by several necrotizing agents, such as ethanol (ED(50)=0.029 mg/kg, p.o.), 0.6 M HCl (ED(50)=0.26 mg/kg, p.o.), and aspirin (ED(50)=0.38 mg/kg, p.o.), and partly inhibited the gastric mucosal lesions induced by indomethacin, but showed no significant effect on acid output in pylorus-ligated rats at doses of 0.05-0.5 mg/kg. The gastroprotection of polygodial was attenuated by pretreatment with indomethacin (10 mg/kg, s.c.), N(G)-nitro-L-arginine methyl ester (70 mg/kg, i.p.), N-ethylmaleimide (10 mg/kg, s.c.) and ruthenium red (3.5 mg/kg, s.c.). Polygodial (0.2 mg/kg, p.o.) increased the amount of reduced glutathione in gastric mucosa of ethanol-treated group. These results suggested that endogenous prostaglandins, nitric oxide, sulfhydryl compounds and vanilloid receptor-mediated effects are involved in the protective effect of polygodial.
Pongpiriyadacha
Yutana
Y
Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Japan.
Matsuda
Hisashi
H
Morikawa
Toshio
T
Asao
Yasunobu
Y
Yoshikawa
Masayuki
M
D000818:Animals; D001241:Aspirin; D000431:Ethanol; D005753:Gastric Mucosa / Q000187:drug effects* / Q000473:pathology; D005765:Gastrointestinal Agents / Q000494:pharmacology* / Q000627:therapeutic use; D006851:Hydrochloric Acid; D007213:Indomethacin; D008297:Male; D009569:Nitric Oxide / Q000502:physiology; D008517:Phytotherapy; D018515:Plant Leaves / Q000737:chemistry; D011453:Prostaglandins / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011955:Receptors, Drug / Q000502:physiology; D012430:Ruthenium Red; D012717:Sesquiterpenes / Q000494:pharmacology* / Q000627:therapeutic use; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D013438:Sulfhydryl Compounds / Q000502:physiology; D032423:Winteraceae / Q000737:chemistry
D005765:Gastrointestinal Agents; D011453:Prostaglandins; D011955:Receptors, Drug; D012717:Sesquiterpenes; D013438:Sulfhydryl Compounds; D012430:Ruthenium Red; D009569:Nitric Oxide; D000431:Ethanol; C034380:polygodial; D006851:Hydrochloric Acid; D001241:Aspirin; D007213:Indomethacin