The role of cholinesterases in rat urinary bladder contractility.
This study examines the effects of inhibition of acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) on acetylcholine (ACh)-induced contraction in rat urinary bladder smooth muscle. Neostigmine, a non-selective ChE inhibitor, caused concentration-dependent contractions in rat urinary bladder strips, whereas tetraisopropylpyrophosphoramide (iso-OMPA; a BuChE inhibitor) failed to affect the resting tone of the preparations. Neostigmine (1 microM) markedly augmented the contractile responses to ACh. Although iso-OMPA (10 microM) also potentiated ACh-induced contraction, the effect was less than that evoked by neostigmine. The activities of AChE in rat urinary bladder strips were significantly (P<0.05) higher than those of BuChE. These results indicated that AChE, rather than BuChE, plays an important role in controlling ACh-induced contractions of rat urinary bladder.
Department of Molecular Pharmacology, Kitasato University School of Pharmaceutical Sciences, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
Tsutomu
T
Nakahara
Yuko
Y
Kubota
Kenji
K
Sakamoto
Kunio
K
Ishii
D000110:Acetylcholinesterase / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D002091:Butyrylcholinesterase / Q000378:metabolism / Q000502:physiology*; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology*; D009130:Muscle, Smooth / Q000502:physiology*; D009388:Neostigmine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013770:Tetraisopropylpyrophosphamide / Q000494:pharmacology; D001743:Urinary Bladder / Q000502:physiology*
D002800:Cholinesterase Inhibitors; D009388:Neostigmine; D013770:Tetraisopropylpyrophosphamide; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase