Cloning of structural gene of Deinococcus radiodurans UV-endonuclease beta.
To characterize its enzymic property we cloned and sequenced the gene of Deinococcus radiodurans encoding UV-endonuclease beta, an alternative enzyme to UvrABC repairing damaged DNA. Amino acid substitutions were found in UV-sensitive mutants. The putative amino acid sequence had some similarity with those of eukaryotic UV-endonucleases and with a sequence found in a protein data base of Bacillus subtilis.
Cell Physiology Laboratory, The Institute of Physical & Chemical Research, Wako, Saitama 350-0198, Japan.
Shigeru
S
Kitayama
Issay
I
Narumi
Tomoo
T
Funayama
Hiroshi
H
Watanabe
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001426:Bacterial Proteins; D001483:Base Sequence; D003001:Cloning, Molecular; D004260:DNA Repair / Q000235:genetics; D045643:DNA Repair Enzymes; D004269:DNA, Bacterial / Q000235:genetics; D030562:Databases, Protein; D034301:Deinococcus / Q000235:genetics*; D004706:Endodeoxyribonucleases / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D016366:Open Reading Frames; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004706:Endodeoxyribonucleases; C055508:UvsD protein, Deinococcus radiodurans; D045643:DNA Repair Enzymes