The effect of an intestinal ischemia-reperfusion injury on renal nerve activity among rats.
An intestinal ischemia-reperfusion injury (IIR) may induce renal tubular dysfunction and a reduction in renal blood flow that may be related to the alteration of renal-nerve activity. A rat model of IIR injury was established. The superior mesenteric artery was clamped for 120 min, constituting the ischemic period, and was then released for 60 min, thus constituting the reperfusion period. Renal-nerve activity, renal function, and hemodynamic changes were recorded during the different periods. The levels of calcitonin gene-related peptide (CGRP) in portal-vein blood and intestinal tissue were investigated here. In the reperfusion period, the efferent renal-nerve activity (ERNA) was markedly elevated (94.3% +/- 21.6% higher than the baseline value), such an elevation being only partially reversed by fluid expansion (29.3% +/- 5.2% higher than the baseline value). The elevation of ERNA contributed to the renal blood-flow reduction from 6.8 +/- 0.3 mL/min/g to 2.0 +/- 0.4 mL/min/g, and decreased diuretic and natriuretic responses. The afferent renal nerve activity (ARNA) was markedly depressed (45.7% +/- 8.1% lower than the baseline value) during the reperfusion period. This depression was not reversed by fluid expansion, suggesting that the baroreflex was not responsible for this effect. The blunted ARNA also contributed to the elevation of ERNA by way of a renorenal reflex. The potent vasodilator neuropeptide in the gut, CGRP, revealed an increased level in the portal-vein blood (92.2 +/- 4.4 pg/mL vs. 57.8 +/- 0.6 pg/mL) and also in intestinal tissue (655.8 +/- 115.9 pg/mL vs. 60.5 +/- 9.4 pg/mL) with a time-matched related pattern with the change to renal-nerve activity, suggesting CGRP's role regarding changes in renal-nerve activity. This study indicates that the elevated ERNA level associated with IIR injury is related to a systemic hypotension-induced baroreflex, the contra-lateral inhibition of ARNA, and possibly also gut-released CGRP. In regards to an IIR injury, the depressed ARNA reflects the involvement of a renal sensory- impairment mechanism.
Lai
I-Rue
IR
Department of Physiology, National Taiwan University College of Medicine, National Taiwan University Hospital, Section 1, Taipei, Taiwan, People's Republic of China.
Ma
Ming-Chieh
MC
Chen
Chau-Fong
CF
Chang
King-Jen
KJ
D000344:Afferent Pathways / Q000503:physiopathology; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000097:blood / Q000378:metabolism; D004195:Disease Models, Animal; D004525:Efferent Pathways / Q000503:physiopathology; D007421:Intestine, Small / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D007511:Ischemia / Q000097:blood / Q000503:physiopathology; D007668:Kidney / Q000294:innervation*; D008297:Male; D011168:Portal System; D051381:Rats; D017208:Rats, Wistar; D012079:Renal Circulation / Q000502:physiology; D015427:Reperfusion Injury / Q000097:blood / Q000503:physiopathology*; D013373:Substance P / Q000097:blood / Q000378:metabolism
D013373:Substance P; D015740:Calcitonin Gene-Related Peptide