[A new method of sputum pre-treatment for PCR preparation].
A rapid sputum processing method was developed for PCR. As a protocol prepared for commercial kit is too much simplified, PCR inhibitor is not completely removed from sputum. We developed a new semi-alkaline protease method to dissolve sputum. From April 2001 to August 2001, 261 sputum samples were treated with our new method. Twenty-one cases (8.0%) were PCR positive and all the results perfectly coincided with the results of the conventional culture method.
Chemo-Sero Therapeutic Research Institute, Gifu University Graduate School of Medicine. masaki@infobears.ne.jp
Takayuki
T
Masaki
Takayuki
T
Ezaki
D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012697:Serine Endopeptidases; D013183:Sputum / Q000382:microbiology*; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000382:microbiology
D011933:Reagent Kits, Diagnostic; D012697:Serine Endopeptidases; C032923:microbial serine proteinases