Thyrocytes, but not C cells, actively undergo growth and folliculogenesis at the periphery of thyroid tissue fragments in three-dimensional collagen gel culture.
In the thyroid, new follicle formation from preexisting follicles (mother follicle-derived folliculogenesis) has been poorly understood. To address this issue, we analyzed mother follicle-derived folliculogenesis, using a thyroid tissue-organotypic culture that retains three-dimensional follicles with both thyrocytes and C cells over a long period. Three types of mother follicle-derived folliculogenesis occurred only at the periphery of the tissue fragments embedded in collagen gel: (1) solid nest, (2) budding and (3) lumen-dividing types. Immunohistochemistry showed that neogenic follicles rarely had calcitonin-positive C cells. Electron microscopy showed that their component thyrocytes expressed normal polarity. In growth, bromodeoxyuridine uptake of thyrocytes at the tissue periphery was about 5 times the uptake that occurred at the center. C cells had no uptake. Thyrotropin (TSH, 10 mU/ml) and free calcium (0.17~1.95 mM), which are associated with the biological behavior of thyrocytes and C cells, respectively, did not affect the events described above. The data indicate that thyrocytes, but not C cells, actively undergo growth and three types of mother follicle-derived folliculogenesis at the tissue periphery in a TSH- or free calcium-independent manner. This suggests that the tissue periphery, which may escape from contact inhibition of cell growth, is the regenerative site.
Toda
Shuji
S
Division of Cellular & Molecular Pathology, Department of Pathology & Biodefence, Saga Medical School, Nabeshima 5-1-1, 849-8501, Saga, Japan, todas@post.saga-med.ac.jp
Aoki
Shigehisa
S
Suzuki
Koichi
K
Koike
Eisuke
E
Ootani
Akifumi
A
Watanabe
Keiko
K
Koike
Norimasa
N
Sugihara
Hajime
H
D000818:Animals; D000963:Antimetabolites / Q000378:metabolism; D001973:Bromodeoxyuridine / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division; D048429:Cell Size; D002478:Cells, Cultured; D008854:Microscopy, Electron; D012038:Regeneration / Q000502:physiology*; D013552:Swine; D013961:Thyroid Gland / Q000166:cytology* / Q000378:metabolism; D013972:Thyrotropin / Q000378:metabolism
D000963:Antimetabolites; D013972:Thyrotropin; D001973:Bromodeoxyuridine; D002118:Calcium