Evaluation of a single dose versus a divided dose regimen of danofloxacin in treatment of Actinobacillus pleuropneumoniae infection in pigs.
A single versus a divided dose regimen of danofloxacin was evaluated in treatment of porcine Actinobacillus pleuropneumoniae infection using clinical observations combined with biochemical infection markers: C-reactive protein, zinc and ascorbic acid. Twenty hours after experimental infection, the 18 pigs received danofloxacin intravenously as a single dose of 2.5mg/kg or four doses of 0.6 mg/kg administered at 24h intervals. These dosage regimens resulted in similar AUCs of the plasma danofloxacin vs time curve. The maximum concentration was 3.5-fold higher using the single dose regimen, while the time with concentrations above the MIC was 2.5-fold longer using the fractionated regimen. Using the single dose regimen, temperature was normalised 32 h post-infection. In contrast, normalisation was delayed until 44 h post-infection using four low doses and a relapse with elevated temperatures at 52 and 68 h was observed. No other significant differences between the treatments were found, neither regarding clinical, haematological nor biochemical observations. The use of the more convenient single dose regimen was appropriate, as it was at least equivalent to the fractionated regimen.
Department of Pharmacology and Pathobiology, The Royal Veterinary and Agricultural University, 9 Ridebanevej, 1870 Frederiksberg C, Copenhagen, Denmark. bla@kvl.dk
Brian
B
Lauritzen
Jens
J
Lykkesfeldt
Christian
C
Friis
D000189:Actinobacillus Infections / Q000097:blood / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary*; D016977:Actinobacillus pleuropneumoniae; D000818:Animals; D000890:Anti-Infective Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics; D019540:Area Under Curve; D001205:Ascorbic Acid / Q000097:blood; D001831:Body Temperature / Q000187:drug effects; D002097:C-Reactive Protein / Q000378:metabolism; D024841:Fluoroquinolones; D007275:Injections, Intravenous / Q000662:veterinary; D007958:Leukocyte Count / Q000662:veterinary; D008297:Male; D008826:Microbial Sensitivity Tests; D011001:Pleuropneumonia / Q000097:blood / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary*; D011897:Random Allocation; D013552:Swine; D013553:Swine Diseases / Q000188:drug therapy* / Q000382:microbiology*; D015032:Zinc / Q000097:blood
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; C068581:danofloxacin; D002097:C-Reactive Protein; D015032:Zinc; D001205:Ascorbic Acid