A novel convenient process to obtain a raw decaffeinated tea polyphenol fraction using a lignocellulose column.
Lignocellulose prepared from sawdust was investigated for its potential application in obtaining a raw decaffeinated tea polyphenol fraction from tea extract. Tea polyphenols having gallate residues, namely, (-)epigallocatechin gallate (EGCg) and (-)epicatechin gallate (ECg), were adsorbed on the lignocellulose column, while caffeine was passed through it. Adsorbed polyphenols were eluted with 60% ethanol, and the elute was found to consist mainly of EGCg and ECg. The caffeine/EGCg ratio was 0.696 before lignocellulose column treatment, but it became 0.004 after the column treatment. These results suggest that the lignocellulose column provides a useful and convenient process of purification of tea polyphenol fraction accompanied by decaffeination.
School of Bioresources, Hiroshima Prefectural University, 562 Nanatsuka, Shobara-shi, Hiroshima 727-0023, Japan. sakanaka@bio.hiroshima-pu.ac.jp
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Sakanaka
D002110:Caffeine / Q000032:analysis* / Q000302:isolation & purification; D028241:Camellia sinensis / Q000737:chemistry*; D002392:Catechin / Q000031:analogs & derivatives* / Q000032:analysis; D002482:Cellulose; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005419:Flavonoids; D006863:Hydrogen-Ion Concentration; D008031:Lignin; D010636:Phenols / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry*; D011108:Polymers / Q000302:isolation & purification*
D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D011108:Polymers; C036909:lignocellulose; D002110:Caffeine; D002392:Catechin; D002482:Cellulose; D008031:Lignin; C062669:epicatechin gallate; C045651:epigallocatechin gallate