Doxycycline inhibits bone resorption by human interface membrane cells from aseptically loose hip replacements.
Matrix metalloproteinases (MMPs) may have a role in the process of aseptic loosening. Doxycycline has been shown to inhibit MMPs. Our aim was to investigate the potential pharmacological effect of doxycycline on aseptic loosening. We used radiolabelled mouse calvariae cultured with human interface membrane cells from aseptically loosened hips. Bone resorption was confirmed in this model. The effect of doxycycline was assessed by culturing dead radiolabelled bone discs with cells from the interface membrane with doxycycline. The control group consisted of the same culture system without doxycycline. Supernatant 45calcium and the total 45calcium remaining in the bone discs at the completion of the culture were used to measure osteolysis. We found that doxycycline can inhibit osteolysis at the interface membrane of aseptically loosened hips. This may have therapeutic implications for the treatment of patients with aseptic loosening of total joint replacements.
Department of Orthopaedics, Glenfield Hospital, Leicester, England, UK.
S M
SM
Ong
G J S
GJ
Taylor
D000368:Aged; D000369:Aged, 80 and over; D000704:Analysis of Variance; D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D019644:Arthroplasty, Replacement, Hip; D001862:Bone Resorption / Q000517:prevention & control*; D002470:Cell Survival; D002478:Cells, Cultured; D004318:Doxycycline / Q000627:therapeutic use*; D005260:Female; D006622:Hip Prosthesis; D006801:Humans; D008297:Male; D061965:Matrix Metalloproteinase Inhibitors; D051379:Mice; D008875:Middle Aged; D015207:Osteoarthritis, Hip / Q000601:surgery; D011475:Prosthesis Failure
D000900:Anti-Bacterial Agents; D061965:Matrix Metalloproteinase Inhibitors; D004318:Doxycycline