A novel method of generating prostate cancer metastases from orthotopic implants.
BACKGROUND
Spontaneous metastasis following implantation at the orthotopic site is a highly desired feature in prostate cancer (CaP) models, since it would enable studies of mechanisms associated with tumor cell dissemination.


METHODS
LuCaP 23.8 and LuCaP 35, hormone-sensitive CaP xenografts that express PSA and the wild-type androgen receptor, were grown orthotopically in SCID mice. When tumor volumes reached approximately 250-500 mg, primary tumors were removed to allow micrometastases to grow.


RESULTS
Using this procedure we generated macroscopic metastases (>20 mg) in 71% (LuCaP 23.8) and 100% (LuCaP 35) of animals, respectively.


CONCLUSIONS
These models may be used to evaluate new treatment modalities and study mechanisms associated with development of metastases.
Corey
Eva
E
University of Washington, Department of Urology, Seattle, Washington 98195, USA. ecorey@u.washington.edu
Quinn
Janna E
JE
Vessella
Robert L
RL
D000818:Animals; D004195:Disease Models, Animal; D006801:Humans; D008113:Liver Neoplasms / Q000556:secondary; D008175:Lung Neoplasms / Q000556:secondary; D008297:Male; D051379:Mice; D016513:Mice, SCID; D009368:Neoplasm Transplantation / Q000379:methods*; D010190:Pancreatic Neoplasms / Q000556:secondary; D017430:Prostate-Specific Antigen / Q000032:analysis; D011471:Prostatic Neoplasms / Q000737:chemistry / Q000556:secondary*; D014183:Transplantation, Heterologous
D017430:Prostate-Specific Antigen