Expression of pigmentation genes following electroporation of albino Monascus purpureus.
A UV-induced albino strain of Monascus purpureus was subjected to electroporation in the presence of genomic DNA from a wild-type red strain of the fungus. Eight colonies expressed color after several weeks of growth. The growth rates of all eight color variants were significantly greater than the recipient and donor strains under some culture conditions. Spectrophotometric analysis of the pigments extracted from the color variants revealed the pigments had absorbance spectra different from the DNA donor strain. These color variants may have resulted from transformation with wild-type DNA, mutation reversion, or activation of alternative pathway(s)-i.e., new mutations-that resulted in pigment production.
Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, Thailand.
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K
Lakrod
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C
Chaisrisook
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DZ
Skinner
D001709:Biotechnology; D004271:DNA, Fungal / Q000032:analysis; D018274:Electroporation; D005516:Food Microbiology; D015966:Gene Expression Regulation, Fungal; D032741:Monascus / Q000235:genetics* / Q000254:growth & development; D009154:Mutation; D010641:Phenotype; D010858:Pigmentation / Q000235:genetics*
D004271:DNA, Fungal