[Visualisation of cyanobacteria in aqueous solution by atomic force microscopy].
Methodical approaches for studying of living cells in aqueous solutions by atomic force microscopy (AFM) are demonstrated. Images of intact cyanobacteria Synechocystis PCC 6803 in TES buffer were captured in tapping mode using aminomodified mica as AFM substrate. Modification of freshly cleaved mica has been done in 3-aminopropyltri-ethoxysilane vapours. The average size of cyanobacteria was determined from AFM images. The linear size of Synechocystis PCC 6803 in TES buffer was equal to 70 x 90 nm and their height was about 20 nm. Possible causes of insufficiently high resolution of the cyanobacteria AFM images in aqueous solutions and possible ways for gaining molecular resolution in studies of structural, functional and micromechanical properties of living cells are discussed.
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D000538:Aluminum Silicates; D002021:Buffers; D000458:Cyanobacteria / Q000648:ultrastructure*; D018625:Microscopy, Atomic Force; D012996:Solutions
D000538:Aluminum Silicates; D002021:Buffers; D012996:Solutions; C011934:mica