Sequence and analysis of pBM02, a novel RCR cryptic plasmid from Lactococcus lactis subsp cremoris P8-2-47.
This paper reports the complete nucleotide sequence of the 3.85 kbp plasmid pBM02 from Lactococcus lactis subsp. cremoris P8-2-47. Analysis of the sequence predicted six ORFs larger than 25 amino acids. They all were transcribed from the same strand and organized in two functional cassettes: the replication region and a putative mobilization region. In the replication region, two ORFs specifying proteins homologous to others found in some classes of rolling circle-replicating plasmids were encountered (copG and repB). In fact, single-stranded DNA was detected as a replication intermediate of pBM02. copG and repB, together with some upstream sequences, formed part of the minimal replication unit of the plasmid. Interestingly, pBM02 shared a 212 bp stretch with plasmids of the pWV01 type, in which the whole single-strand origin of replication is included. In the mobilization region, an ORF coding for a mobilization-like protein was present, preceded by a putative oriT sequence homologous to that of plasmid pMV158. The replicon of pBM02 is of the wide-host range type, and functions in both Gram-positive and Gram-negative bacteria, including Lactobacillus casei, Lactobacillus plantarum, Bacillus subtilis, and Escherichia coli.
Instituto de Productos Lácteos de Asturias (CSIC), Carretera de Infiesto s/n, 33300-Villaviciosa, Asturias, Spain.
Claudia
C
Sánchez
Baltasar
B
Mayo
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D004265:DNA Helicases; D004261:DNA Replication / Q000235:genetics; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins / Q000235:genetics; D017403:In Situ Hybridization; D013294:Lactococcus lactis / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010957:Plasmids / Q000235:genetics*; D011506:Proteins / Q000235:genetics; D018741:Replication Origin; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D015534:Trans-Activators; D020506:Untranslated Regions
D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D011506:Proteins; C074125:RepB' protein, E coli; D015534:Trans-Activators; D020506:Untranslated Regions; C045264:replication initiator protein; D004247:DNA; D004265:DNA Helicases