Human B-blast specific target determinants in CML: a methodological study.
A method for preparation of purified human PWM stimulated B lymphoblasts and their usefulness as targets in CML is described. The purified B lymphoblasts were found to carry surface membrane immunoglobulin (SmIg) and HLA-DR antigens and were non E-rosette forming. In contrast, PHA-stimulated lymphoblasts were found to be E-rosetting, SmIg negative and without serologically detectable HLA-DR antigens, and thus were characterized as T lymphoblasts. Using B and T lymphoblasts in parallel as targets in CML, it was possible to demonstrate target determinants exclusively expressed by the B lymphoblasts.
The Tissue Typing Laboratory, Blood Bank and Blood Grouping Laboratory, Aarhus Kommunehospital, University of Aarhus, Denmark.
H E
HE
Johnsen
D001402:B-Lymphocytes / Q000737:chemistry / Q000166:cytology* / Q000276:immunology*; D015415:Biomarkers; D002469:Cell Separation / Q000379:methods*; D006684:HLA-DR Antigens / Q000032:analysis / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000737:chemistry / Q000187:drug effects / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000737:chemistry / Q000276:immunology
D015415:Biomarkers; D006684:HLA-DR Antigens; D010835:Phytohemagglutinins