Rhinovirus induction of the CXC chemokine epithelial-neutrophil activating peptide-78 in bronchial epithelium.
Epithelial-neutrophil activating peptide-78 (ENA-78) induces neutrophil migration, an early response to viral infection. Rhinovirus serotype 16 (RV16) was used to infect primary bronchial epithelial cells and a cell line (BEAS-2B). Release of ENA-78 protein was measured by enzyme-linked immunosorbent assay, ENA-78 mRNA production was quantified by reverse-transcription polymerase chain reaction, and ENA-78 promoter activity was assessed by use of a promoter construct. After infection with RV16, ENA-78 protein and mRNA increased significantly, and RV16 induced 3-fold increases in ENA-78 gene transcription. Nasal ENA-78 measured in patients with asthma with and without RV infection was more elevated in patients with RV infection present. Our study demonstrates that ENA-78 is produced in bronchial epithelial cells in response to RV16 infection. With other chemokines, it may be an important initiator of neutrophil airway inflammation during RV common colds and thus may play a role in the development of virus-associated airway pathologies.
Donninger
Howard
H
Lung and Allergy Unit and Medical Virology, University of Stellenbosch, Cape Town, South Africa.
Glashoff
Richard
R
Haitchi
Hans-Michael
HM
Syce
James A
JA
Ghildyal
Reena
R
van Rensburg
Estrelita
E
Bardin
Philip G
PG
D000328:Adult; D001249:Asthma / Q000378:metabolism; D001980:Bronchi / Q000378:metabolism*; D002460:Cell Line; D002478:Cells, Cultured; D054365:Chemokine CXCL5; D019743:Chemokines, CXC / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D016209:Interleukin-8 / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D008297:Male; D010850:Picornaviridae Infections / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012229:Rhinovirus / Q000502:physiology*; D013997:Time Factors; D014158:Transcription, Genetic; D014779:Virus Replication
C486263:CXCL5 protein, human; D054365:Chemokine CXCL5; D019743:Chemokines, CXC; D016209:Interleukin-8; D012333:RNA, Messenger