Sinorhizobium americanus sp. nov., a new Sinorhizobium species nodulating native Acacia spp. in Mexico.
The sinorhizobia isolated from root nodules of Acacia species native of Mexico constitute a diverse group of bacteria on the basis of their metabolic enzyme electromorphs but share restriction patterns of the PCR products of 16S rRNA genes and a common 500 kb symbiotic plasmid. They are distinguished from other Sinorhizobium species by their levels of DNA-DNA hybridization and the sequence of 16S rRNA and nifH genes. nolR gene hybridization patterns were found useful to identify sinorhizobia and characterize species. A new species, Sinorhizobium americanus, is described and the type strain is CFNEI 156 from Acacia acatlensis.
Toledo
Ivonne
I
Centro de Investigación sobre Fijación de Nitrógeno, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México.
Lloret
Lourdes
L
Martínez-Romero
Esperanza
E
D000045:Acacia / Q000145:classification / Q000382:microbiology*; D001483:Base Sequence; D024901:Drug Resistance, Multiple, Bacterial; D004798:Enzymes / Q000032:analysis / Q000145:classification / Q000378:metabolism; D007612:Kanamycin / Q000494:pharmacology; D008800:Mexico; D009268:Nalidixic Acid / Q000494:pharmacology; D009586:Nitrogen Fixation; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D020579:Sinorhizobium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure; D012988:Soil Microbiology
D004798:Enzymes; D012336:RNA, Ribosomal, 16S; D009268:Nalidixic Acid; D007612:Kanamycin