Maize (Zea mays)-derived bovine trypsin: characterization of the first large-scale, commercial protein product from transgenic plants.
Bovine trypsin (EC 3.4.21.4) is an enzyme that is widely used for commercial purposes to digest or process other proteins, including some therapeutic proteins. The biopharmaceutical industry is trying to eliminate animal-derived proteins from manufacturing processes due to the possible contamination of these products by human pathogens. Recombinant trypsin has been produced in a number of systems, including cell culture, bacteria and yeast. To date, these expression systems have not produced trypsin on a scale sufficient to fulfill the need of biopharmaceutical manufacturers where kilogram quantities are often required. The present paper describes commercial-level production of trypsin in transgenic maize (Zea mays) and its physical and functional characterization. This protease, the first enzyme to be produced on a large-scale using transgenic plant technology, is functionally equivalent to native bovine pancreatic trypsin. The availability of this reagent should allow for the replacement of animal-derived trypsin in the processing of pharmaceutical proteins.
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Susan L
SL
Woodard
Jocelyne M
JM
Mayor
Michele R
MR
Bailey
Donna K
DK
Barker
Robert T
RT
Love
Jeffrey R
JR
Lane
Donna E
DE
Delaney
Janet M
JM
McComas-Wagner
Hanuman D
HD
Mallubhotla
Elizabeth E
EE
Hood
Lawrence J
LJ
Dangott
Shane E
SE
Tichy
John A
JA
Howard
D000818:Animals; D002417:Cattle; D003001:Cloning, Molecular; D004789:Enzyme Activation; D005433:Flour; D006031:Glycosylation; D006801:Humans; D007700:Kinetics; D030821:Plants, Genetically Modified / Q000737:chemistry / Q000201:enzymology*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D012639:Seeds / Q000737:chemistry / Q000201:enzymology; D014357:Trypsin / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D014362:Trypsinogen / Q000378:metabolism; D003313:Zea mays / Q000737:chemistry / Q000201:enzymology / Q000235:genetics*
D011994:Recombinant Proteins; D014362:Trypsinogen; D014357:Trypsin