Isolation of stress-related genes of rubber particles and latex in fig tree (Ficus carica) and their expressions by abiotic stress or plant hormone treatments.
Two rubber particle protein genes and one latex gene in fig tree (Ficus carica) have been isolated and their expression following various abiotic stress treatments have been investigated. The two major proteins that are tightly associated with the catalytically active rubber particles have been sequenced to be peroxidase (POX) and trypsin inhibitor (TRI). A cDNA encoding a basic class I chitinase (CHI) has also been isolated from the fig tree latex. Wounding treatment strongly induced the expression of the three stress-related genes. Among the abiotic stresses investigated, drought treatment greatly induced the expression of POX, whereas the expression of CHI and TRI decreased after the same treatment. Cold treatment reduced slightly the transcript levels of the thee genes, and NaCl reduced marginally the expression of CHI. The expression of POX, CHI, and TRI was induced by jasmonic acid and abscisic acid, by jasmonic acid, and by salicylic acid, respectively. Different expression of the stress-related genes following various abiotic stress or plant hormone treatments suggests that a crosstalk exists between the signal transduction pathways elicited by abiotic stresses and hormones in plants. Our present results showing the expression of stress-related proteins on the surface of rubber particles and latex in F. carica also imply the possible role of rubber particles and latex in defense in rubber-producing plant species.
Kim
Jin Sun
JS
Division of Applied Plant Science, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, 500-757 Korea.
Kim
Yeon Ok
YO
Ryu
Hyun Ju
HJ
Kwak
Yeon Sig
YS
Lee
Ji Yeon
JY
Kang
Hunseung
H
D001483:Base Sequence; D002688:Chitinases / Q000235:genetics / Q000378:metabolism; D030681:Ficus / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D007840:Latex / Q000378:metabolism*; D008969:Molecular Sequence Data; D009195:Peroxidase / Q000235:genetics / Q000378:metabolism; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012408:Rubber / Q000378:metabolism*; D013314:Stress, Mechanical; D014361:Trypsin Inhibitors; D000516:alpha-Amylases / Q000037:antagonists & inhibitors
D007840:Latex; D010937:Plant Growth Regulators; D010940:Plant Proteins; D014361:Trypsin Inhibitors; D012408:Rubber; D009195:Peroxidase; D000516:alpha-Amylases; D002688:Chitinases