[Study on the molecular mechanism of genetic damages in body cells in mice treated with organic mutants in drinking water].
The genetic toxicity of some volatile compounds of chlorinated by-products have been determined in animal studies. But little is known at present about the genetic toxicity of nonvolatile matter in drinking water in vivo assay. In a sub-acute experiment, the mice were exposed to the organic compounds extracted from in-home tap water. The pathologic changes in the liver and kidney tissues were observed under microscope and electron microscope. The frequencies of micronuclei in polychromatic erythrocytes in mice chest bone marrow in the three treated groups were tested. The point mutants in p53 gene of liver, kidney and colon tissues in the high dosage group were examined by PCR-SSCP. Especially, the frequencies of mutations in exon 7 of p53 gene in those mice were higher than those in exon 5 of p53 gene. It showed that the study on the molecular genetic toxicity of organic mutants in vivo assay was possible.
School of Public Health, Tongji Medical University, Wuhan 430030, China.
J
J
Yuan
W
W
Dai
X
X
Li
X
X
Chen
D000818:Animals; D005260:Female; D016158:Genes, p53; D007668:Kidney / Q000648:ultrastructure; D008099:Liver / Q000648:ultrastructure; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D009153:Mutagens / Q000302:isolation & purification / Q000633:toxicity*; D017354:Point Mutation; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D014881:Water Supply / Q000032:analysis*
D009153:Mutagens; D014874:Water Pollutants, Chemical