Ascorbic acid against reperfusion injury in human renal transplantation.
The cadaveric renal graft is exposed to ischaemic injury during preservation and to oxidative damage during reperfusion. Both these mechanisms are known to cause cell damage, which may impair graft function. Reperfusion injury (RPI) is mediated by reactive oxygen species (ROS). Ascorbic acid (AA) is a potent physiological extracellular scavenger of ROS. We perfused 31 renal grafts immediately before implantation with a solution of Euro-Collins containing 0.5 mg/ml of AA to diminish RPI. From every donor, the contralateral kidney served as a control. The control grafts were perfused with the same perfusion as those of the AA group, only without the AA substitution. We assessed the effect of AA by recording serum creatinine, creatinine clearance, initial graft function and early rejections. The incidence of delayed graft function (DGF) was 32% in the AA group, and 29% in the control group. Other parameters were also similar in both groups, except for the length of DGF, which showed a trend towards a shorter duration in the AA group. The pre-operative systemic AA concentration was significantly ( P=0.01) lower in the haemodialysis patients than in those on peritoneal dialysis. In conclusion, this clinical study could not demonstrate significant benefits of AA in renal transplantation.
Department of Anaesthesia, Fourth Department of Surgery, Helsinki University Central Hospital, P.O. Box 263, 00029 HUS, Helsinki, Finland. karri.norio@hus.fi
Karri
K
Norio
Mårten
M
Wikström
Kaija
K
Salmela
Lauri
L
Kyllönen
Leena
L
Lindgren
D000368:Aged; D000975:Antioxidants / Q000627:therapeutic use*; D001205:Ascorbic Acid / Q000627:therapeutic use*; D006085:Graft Survival / Q000187:drug effects; D006801:Humans; D007668:Kidney / Q000187:drug effects* / Q000502:physiology; D016030:Kidney Transplantation; D008875:Middle Aged; D010477:Perfusion; D010865:Pilot Projects; D015427:Reperfusion Injury / Q000517:prevention & control*
D000975:Antioxidants; D001205:Ascorbic Acid