Functional analysis of two matrix attachment region (MAR) elements in transgenic maize plants.
Matrix attachment regions (MARs) are binding sites for nuclear scaffold proteins in vitro, and are proposed to mediate the attachment of chromatin to the nuclear scaffold in vivo. Previous reports suggest that MAR elements may stabilize transgene expression. Here, we tested the effects of two maize MAR elements (P-MAR from the P1-rr gene, and Adh1-MAR from the adh1 gene) on the expression of a gusA reporter gene driven by three different promoters: the maize p1 gene promoter, a wheat peroxidase (WP) gene promoter, or a synthetic promoter (Rsyn7). The inclusion of P-MAR or Adh1-MAR on P::GUS transgene constructs did not reduce variation in the levels of GUS activity among independent transformation events, nor among the progeny derived from each event. The Adh1-MAR element did not affect GUS expression driven by the WP promoter, but did modify the spatial pattern of expression of the Rsyn7::GUS transgene. These results indicate that, in transgenic maize plants, the effects of MAR elements can vary significantly depending upon the promoter used to drive the transgene.
Sidorenko
Lyudmila
L
Department of Zoology and Genetics, Iowa State University, IA 50011, Ames, USA.
Bruce
Wesley
W
Maddock
Sheila
S
Tagliani
Laura
L
Li
Xianggan
X
Daniels
Michael
M
Peterson
Thomas
T
D000426:Alcohol Dehydrogenase / Q000235:genetics; D005966:Glucuronidase / Q000235:genetics; D045170:Matrix Attachment Regions; D010544:Peroxidases / Q000235:genetics; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D019076:Transgenes; D003313:Zea mays / Q000235:genetics*
D000426:Alcohol Dehydrogenase; D010544:Peroxidases; C070865:wheat peroxidase; D005966:Glucuronidase