Chemical compositional separation of styrene-methyl methacrylate copolymers using high-performance liquid chromatography with liquefied carbon dioxide as eluent.
Chromatographic separation of styrene-methyl methacrylate (MMA) copolymers depending on the chemical composition was studied using liquefied carbon dioxide as an adsorption promoting solvent, and tetrahydrofuran, chloroform containing ethanol as a desorption promoting solvent in the mobile phase and the column packed non-bonded silica gel by a solvent gradient method. With the increase of MMA content, the elution was retarded indicating that the typical normal-phase type of adsorption occurred. The effects of type of desorption solvents, molecular mass of sample, and column temperature on the elution were investigated.
Kawai
Eiichi
E
Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan.
Shimoyama
Kumiko
K
Ogino
Kenji
K
Sato
Hisaya
H
D002245:Carbon Dioxide / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D020366:Methylmethacrylate / Q000737:chemistry*; D008970:Molecular Weight; D011108:Polymers / Q000302:isolation & purification*; D012997:Solvents; D020058:Styrene / Q000737:chemistry*; D013696:Temperature
D011108:Polymers; D012997:Solvents; D002245:Carbon Dioxide; D020366:Methylmethacrylate; D020058:Styrene