Comparative analysis of mononuclear cell surface markers in atopic processes--a preliminary study.
Atopic disorders are driven by the Th2 cell subset. We have determined the expression of costimulatory molecules and cell surface markers on peripheral CD4+ T cells and antigen presenting cells, in different atopic diseases, and we have also tried to correlate the expression of these markers with the severity of the disease. Cells from patients with atopic and contact dermatitis, mild or severe asthma, and symptomatic and non-symptomatic atopic rhinitis were analyzed by flow cytometry. Our results showed that CD30, CD124, and CD152 expression on CD4+ T cells was significantly higher in atopic dermatitis than in contact dermatitis patients (p < 0.05). It was interesting to observe that the cell surface expression of CD80 in T and B cells from atopic dermatitis patients was not enhanced as opposed to the other atopic diseases we analyzed. Our results suggest that there are differences in the immune mechanisms involved in the different atopic diseases, and that expression of CD30 in CD4+ T cells might be a marker of disease activity in atopic dermatitis.
Departamento de Bioquímica, Instituto Nacional de Enfermedades Respiratorias, Tlalpan, México.
Yonathan
Y
Garfias
Enrique
E
Rojas-Ramos
María del Carmen
Mdel C
Jiménez
Salvador
S
Martínez-Cairo
Raúl
R
Chávez
Patricia
P
Gorocica
Edgar
E
Zenteno
Ricardo
R
Lascurain
D000328:Adult; D000938:Antigen-Presenting Cells / Q000276:immunology* / Q000378:metabolism; D015703:Antigens, CD; D018941:Antigens, CD19 / Q000096:biosynthesis; D000943:Antigens, Differentiation / Q000096:biosynthesis; D000954:Antigens, Surface; D018122:B7-1 Antigen / Q000096:biosynthesis; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D060908:CTLA-4 Antigen; D005434:Flow Cytometry; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology* / Q000378:metabolism; D017730:Ki-1 Antigen / Q000096:biosynthesis; D018950:Lipopolysaccharide Receptors / Q000096:biosynthesis; D019948:Receptors, Interleukin-4 / Q000096:biosynthesis
D015703:Antigens, CD; D018941:Antigens, CD19; D000943:Antigens, Differentiation; D000954:Antigens, Surface; D018122:B7-1 Antigen; D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human; D017730:Ki-1 Antigen; D018950:Lipopolysaccharide Receptors; D019948:Receptors, Interleukin-4