[Reversed-phase high performance liquid chromatography of the products of microbiological degradation of toluene ].
Conditions have been selected for a reversed-phase high-performance liquid chromatographic assay of intermediate products formed in the course of utilization of toluene by Pseudomonas putida. The composition of products indicates that degradation of toluene by strain BS590-P proceeds primarily through the formation of benzoate and catechol. This is followed by degradation of catechol via ortho-cleavage. In strain BS3701-P, toluene oxidation involves both the side chain and the aromatic ring.
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, prosp. Nauki 5, Pushchino, Moscow Oblast, 142290 Russia. zelen@ibpm.serpukhov.su
N F
NF
Zelenkova
M U
MU
Arinbasarov
D001565:Benzoates / Q000032:analysis / Q000378:metabolism; D019817:Benzoic Acid / Q000032:analysis / Q000378:metabolism; D002396:Catechols / Q000032:analysis / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D010084:Oxidation-Reduction; D016958:Pseudomonas putida / Q000378:metabolism*; D014050:Toluene / Q000378:metabolism*
D001565:Benzoates; D002396:Catechols; D014050:Toluene; D019817:Benzoic Acid; C034221:catechol