Quantitative determination of free intracellular alpha-keto acids in neutrophils.
For the first time, a procedure is described for the quantitative analysis of free alpha-keto acid content in human neutrophils (PMNs) relative to single cell number by reversed-phase fluorescence high-performance liquid chromatography. The procedure is minimally invasive and is unsurpassed in the quality of PMN separation, ease of sample preparation as well as sample stability. This method can satisfy the rigorous demands for an ultra-sensitive, comprehensive and rapid intracellular alpha-keto acid analysis in particularly for the surveillance of severe diseases as well as cellular or organ dysfunction.
Department of Anaesthesiology, Intensive Medicine and Pain Therapy Justus Liebig University, Rudolf-Buchheim-Strasse 7, 35390, Giessen, Germany.
Jörg
J
Mühling
Markus
M
Fuchs
Marie E
ME
Campos
Jens
J
Gonter
Jörg M
JM
Engel
Armin
A
Sablotzki
Thilo
T
Menges
Stefan
S
Weiss
Marius G
MG
Dehne
Matthias
M
Krüll
Gunter
G
Hempelmann
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007651:Keto Acids / Q000097:blood*; D009504:Neutrophils / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods
D007651:Keto Acids