Reversed-phase liquid chromatographic method for the determination of selected room-temperature ionic liquid cations.
The separation of selected 1-alkyl- and 1-aryl-3-methylimidazolium-based room temperature ionic liquid cations has been performed using reversed-phase high-performance liquid chromatography with electrospray ionization mass detection. The RP-HPLC method development started with the selection of a column taking into account especially the resolution of low molecular congeners of the selected group. Mobile phase composition was optimized for peak resolution, sensitivity and high reproducibility of retention values. The results of the method development were applied to the determination of exemplary ionic liquid species present in the medium used in cytotoxicity studies.
Faculty of Chemistry, Waste Management Laboratory, University of Gdańsk, ul. Sobieskiego 18, PL 80-952 Gdańsk, Poland. sox@chem.univ.gda.pl
Piotr
P
Stepnowski
Anja
A
Müller
Peter
P
Behrend
Johannes
J
Ranke
Jens
J
Hoffmann
Bernd
B
Jastorff
D002412:Cations; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization; D013696:Temperature
D002412:Cations