Mucosally-derived HPV-40 can infect both human genital foreskin and cutaneous hand skin tissues grafted into athymic mice.
HPV-40 is a rare HPV type that has been detected only in genital mucosal tissues. This HPV type is very closely related to HPV-7, which has a predominantly cutaneous tissue tropism. We have shown, previously, that an isolate of HPV-40 (described here as HPV-40(Hershey) or HPV-40(H)) productively infected genital tissues. In this study, HPV-40(H) was tested for productive infection of cutaneous tissue. Fetal hand skin fragments were incubated with infectious HPV-40(H) and implanted subrenally into athymic mice. After 120 days, xenografts showed morphological changes consistent with HPV-40(H) infection and were HPV-40 DNA in situ positive and capsid antigen positive. The results demonstrated that hand skin can support HPV-40(H) infection thereby indicating that this viral type has the capacity to infect both genital mucosal and cutaneous tissues.
Department of Comparative Medicine, College of Medicine, The Milton S. Hershey Medical Center of the Pennsylvania State University, 500 University Drive, Hershey, PA 17033, USA.
Audrey L
AL
Jenkins
C Max
CM
Lang
Lynn R
LR
Budgeon
Nancy M
NM
Cladel
Cynthia A
CA
Reed
Danny R
DR
Welch
Neil D
ND
Christensen
Audrey J
AJ
Jenkins
D000595:Amino Acid Sequence; D000818:Animals; D016332:Fetal Tissue Transplantation / Q000473:pathology; D006225:Hand; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data; D009092:Mucous Membrane / Q000821:virology*; D027383:Papillomaviridae / Q000302:isolation & purification / Q000472:pathogenicity*; D010413:Penis / Q000821:virology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012867:Skin / Q000473:pathology / Q000821:virology*; D016038:Skin Transplantation / Q000473:pathology*; D014183:Transplantation, Heterologous / Q000473:pathology; D014764:Viral Proteins / Q000737:chemistry*
D014764:Viral Proteins