Flow injection analysis: Rayleigh light scattering technique for total protein determination.
A novel flow injection analysis (FIA) method with Rayleigh light scattering (RLS) detection was developed for the determination of total protein concentrations. This method is based on the weak intensity of RLS of bromothymol blue (BB) (3',3"-dibromothymolsulfonephthalein) which can be enhanced by the addition of protein in weakly acidic solution. A common spectrofluorimeter was used as a detector. It was proved that the application of this method to quantify the total proteins in real samples by using bovine serum albumin was possible. The RLS signal was detected at lambda(ex)= lambda(em)=572 nm. The linear range was 7.0-70.0 microg mL(-1), the detection limit was 3.75 microg mL(-1), the reproducibility was 5.5% (n=7), and the sample throughput was 26 h(-1).
Vidal
Ezequiel
E
FIA Laboratory, Department of Chemistry, Universidad Nacional del Sur, Av. Alem 1253, 8000 Bahía Blanca, Argentina.
Palomeque
Miriam E
ME
Lista
Adriana G
AG
Fernández Band
Beatriz S
BS
D000818:Animals; D002138:Calibration; D002417:Cattle; D004396:Coloring Agents; D017022:Flow Injection Analysis / Q000379:methods*; D008027:Light; D011506:Proteins / Q000032:analysis*; D012542:Scattering, Radiation; D012710:Serum Albumin, Bovine / Q000032:analysis; D013050:Spectrometry, Fluorescence; D014556:Urine / Q000737:chemistry
D004396:Coloring Agents; D011506:Proteins; D012710:Serum Albumin, Bovine