The plasma membrane-associated NADH oxidase (ECTO-NOX) of mouse skin responds to blue light.
NADH oxidases of the external plasma membrane surface (ECTO-NOX proteins) are characterized by oscillations in activity with a regular period length of 24 min. Explants of mouse skin exhibit the oscillatory activity as estimated from the decrease in A(340) suggesting that individual ECTO-NOX molecules must somehow be induced to function synchronously. Transfer of explants of mouse skin from darkness to blue light (495 nm, 2 min, 50 micromol m(-1) s(-1)) resulted in initiation of a new activity maximum (entrainment) with a midpoint 36 min after light exposure followed by maxima every 24 min thereafter. Addition of melatonin resulted in a new maximum 24 min after melatonin addition. The findings suggest that the ECTO-NOX proteins play a central role in the entrainment of the biological clock both by light and by melatonin.
Morré
D James
DJ
Department of Medicinal Chemistry and Molecular Pharmacology, Hansen Life Sciences Research Building, Purdue University, 201 S. University Street, West Lafayette, IN 47907-2064, USA. moore@pharmacy.purdue.edu
Morré
Dorothy M
DM
D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D003624:Darkness; D007700:Kinetics; D008027:Light; D051379:Mice; D008810:Mice, Inbred C57BL; D009097:Multienzyme Complexes / Q000378:metabolism / Q000528:radiation effects*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism / Q000528:radiation effects*; D009924:Organ Culture Techniques; D012867:Skin / Q000201:enzymology* / Q000528:radiation effects
D009097:Multienzyme Complexes; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases