Plasma lipoproteins in transport of silibinin, an antioxidant flavonolignan from Silybum marianum.
To assess the role of plasma lipoproteins in the transport of silibinin, an antioxidant flavonolignan, (125)I-labelled silibinin ((125)I-SB) administered perorally to the rat was used. The plasma (125)I-SB derived radioactivity was distributed among plasma lipoproteins according to their lipophilicity (TAG-rich lipoproteins 30-40% > LDL 15% > HDL 5%), and in the fraction of d > 1.215 containing albumin and other proteins a minority amount of radioactivity was found. Administration of (125)I-SB in a complex with phosphatidylcholine resulted in proportionally higher radioactivities in all fractions as well as in tissues. Dietary olive oil had a slightly decreasing effect on plasma concentrations of silibinin measured by HPLC as well as on (125)I-SB derived radioactivity in plasma and liver. In the TAG-rich lipoprotein fraction and HDL no effects of olive oil on the levels of (125)I-SB derived radioactivities were observed, however, at a 30 min interval the levels of (125)I-SB derived radioactivity in LDL and the heart were significantly decreased in the olive oil group. These results suggest that (i) silibinin is not resorbed by the chylomicron pathway, and (ii) the endogenous lipoprotein pathway VLDL --> LDL may play a role in the transport of silibinin from the liver to the extrahepatic tissues concurrently facilitating the lipoprotein antioxidant influence of silibinin.
Institute of Medical Chemistry, Palacky University, Olomouc, Czech Republic.
Zdenek
Z
Svagera
Nina
N
Skottová
Petr
P
Vána
Rostislav
R
Vecera
Karel
K
Urbánek
Marie
M
Belejová
Pavel
P
Kosina
Vilím
V
Simánek
D000818:Animals; D000975:Antioxidants / Q000493:pharmacokinetics; D001692:Biological Transport; D005419:Flavonoids / Q000493:pharmacokinetics; D044948:Flavonols; D007457:Iodine Radioisotopes / Q000493:pharmacokinetics; D008074:Lipoproteins / Q000097:blood*; D008099:Liver / Q000378:metabolism; D008297:Male; D020944:Silybum marianum; D010936:Plant Extracts / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D000077385:Silybin; D012838:Silymarin / Q000097:blood / Q000493:pharmacokinetics*
D000975:Antioxidants; D005419:Flavonoids; D044948:Flavonols; D007457:Iodine Radioisotopes; D008074:Lipoproteins; D010936:Plant Extracts; D012838:Silymarin; D000077385:Silybin