Metabolic engineering of beta-lactam production.
Metabolic engineering has become a rational alternative to classical strain improvement in optimisation of beta-lactam production. In metabolic engineering directed genetic modification are introduced to improve the cellular properties of the production strains. This has resulted in substantial increases in the existing beta-lactam production processes. Furthermore, pathway extension, by heterologous expression of novel genes in well-characterised strains, has led to introduction of new fermentation processes that replace environmentally damaging chemical methods. This minireview discusses the recent developments in metabolic engineering and the applications of this approach for improving beta-lactam production.
Thykaer
Jette
J
Center for Process Biotechnology, BioCentrum, Technical University of Denmark, Building 223, DK-2800, Lyngby, Denmark.
Nielsen
Jens
J
D000900:Anti-Bacterial Agents / Q000378:metabolism; D019149:Bioreactors / Q000382:microbiology*; D002511:Cephalosporins / Q000096:biosynthesis; D002513:Cephamycins / Q000096:biosynthesis; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal / Q000235:genetics / Q000502:physiology*; D007218:Industrial Microbiology / Q000379:methods*; D003904:Mitosporic Fungi / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D008954:Models, Biological; D010406:Penicillins / Q000096:biosynthesis; D015202:Protein Engineering / Q000379:methods*; D013302:Streptomyces / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D047090:beta-Lactams / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D002513:Cephamycins; D010406:Penicillins; D047090:beta-Lactams; C025163:cephalosporin C; C024925:cephamycin C