[The influence of type I collagen on the cell behavior of human embryonic periosteous osteoblasts].
OBJECTIVE
To study the influence of type I collagen (COL I) on the cell behavior of human periosteous osteoblasts (OB) and the application of type I collagen in constructing bioactive artifical bone.


METHODS
OB were cultured on dishes coated with bovine type I collagen in different final concentrations. The cell adhesion was examined by the methods of cell count, the proliferation of OB was studied by 3H-TdR, and the osteoblastic ability was assessed by the synthesis of collagen, osteocalcin and alkaline phosphatase (ALP).


RESULTS
OB cultured on type I collagen layer had the following characteristics: 1. The amounts of adhesive cells were maximal top in 25 micrograms/ml final concentration; 2. The proliferation of OB was decreased above 12.5 micrograms/ml final concentration (P < 0.05); 3. The synthesis of type I collagen was reduced slightly (above 25 micrograms/ml, P < 0.05); 4. The secretion of osteocalcin was increased markedly (above 6.25 micrograms/ml, P < 0.05, which reached maximally in 25 micrograms/ml); 5. The ALP activity was also increased (above 12.5 micrograms/ml, P < 0.05).


CONCLUSIONS
Type I collagen promotes the expression of osteoblastic phenotype and cell adhesion. When the scaffold materials for bone tissue engineering are coated with type I collagen, the osteogenesis of OB is enhanced to accelerate the transformation course from artificial bone to biological bone, the best final concentration is 25 micrograms/ml.
Yang
Z
Z
Department of Orthopedic Surgery, First Affiliated Hospital, WCUMS, Chengdu 610041, China.
Yu
X
X
Huang
F
F
Xie
H
H
D000469:Alkaline Phosphatase / Q000096:biosynthesis; D002448:Cell Adhesion; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000494:pharmacology*; D004622:Embryo, Mammalian; D006801:Humans; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D015675:Osteocalcin / Q000096:biosynthesis; D010521:Periosteum / Q000166:cytology*; D023822:Tissue Engineering
D024042:Collagen Type I; D015675:Osteocalcin; D000469:Alkaline Phosphatase