High IgM production by human-human hybridoma HB4C5 cells cultured in microtubes.
HB4C5 cells, a human-human hybridoma, were cultured in microtubes. After 24 h of cultivation, growth of the cells cultured in microtubes was 1.2- to 1.5-fold that in culture dishes or 96-well culture plates. The production of IgM was 2.6- to 3.3-fold that in the 96-well culture plates.
Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, Japan. mars95@agr.ehime-u.ac.jp
Takuya
T
Sugahara
Satomi
S
Yano
Takeshi
T
Sasaki
D002452:Cell Count; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002478:Cells, Cultured; D003470:Culture Media; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D007075:Immunoglobulin M / Q000096:biosynthesis*; D013997:Time Factors
D003470:Culture Media; D007075:Immunoglobulin M