Correlation between the avidity maturation of anti-HSP70 IgG autoantibody and recombination activating gene expressions in peripheral lymphoid tissues of Toxoplasma gondii-infected mice.
The avidity maturation of anti-TgHSP70 IgG antibody produced by B-2 cells of BALB/c mice (a resistant strain) and that of anti-mHSP70 IgG autoantibody produced by B-1 cells of C57BL/6 mice (B6; a susceptible strain) was observed after Toxoplasma gondii infection. Recombination-activating genes (RAGs) were predominantly expressed in B-1 cells from peritoneal exudate cells (PECs) of T. gondii-infected B6 mice, while RAGs were expressed in B-2 cells from PECs of BALB/c mice. These results suggest that the involvement of RAG gene activations in the peripheral lymphoid tissues in the avidity maturation of anti-TgHSP70 IgG antibody and anti-mHSP70 IgG autoantibody in T. gondii-infected mice.
Department of Infection and Host Defense, Graduate School of Medicine, Chiba University, Chiba, Chiba 260-8670, Japan.
Mei
M
Chen
Fumie
F
Aosai
Hye-Seong
HS
Mun
Kazumi
K
Norose
Lian Xun
LX
Piao
Akihiko
A
Yano
D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis / Q000276:immunology*; D000915:Antibody Affinity; D001323:Autoantibodies / Q000096:biosynthesis / Q000276:immunology*; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D015870:Gene Expression; D018840:HSP70 Heat-Shock Proteins / Q000276:immunology* / Q000302:isolation & purification; D006360:Heat-Shock Proteins / Q000378:metabolism; D018398:Homeodomain Proteins / Q000096:biosynthesis* / Q000235:genetics; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology; D008221:Lymphoid Tissue / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D015800:Protozoan Proteins; D014124:Toxoplasmosis, Animal / Q000276:immunology*
D000913:Antibodies, Protozoan; D001323:Autoantibodies; C106179:BAG1 protein, Toxoplasma; D004268:DNA-Binding Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D018398:Homeodomain Proteins; D007074:Immunoglobulin G; D015800:Protozoan Proteins; C499308:Rag2 protein, mouse; C064655:V(D)J recombination activating protein 2; C064658:RAG-1 protein