Inhibition of tumor rejection by gammadelta T cells and IL-10.
Although many tumors express tumor-specific antigens, most fail to stimulate effective immune responses. Tumors generally lack co-stimulatory molecules, which can lead to tolerance of tumor-specific T cells and progressive tumor growth. Here, we demonstrate that the ovalbumin (OVA) transfected EL4 tumor, E.G7-OVA, grows progressively in syngeneic mice even though the tumor can be rejected if the mice are immunized with OVA in adjuvant. E.G7-OVA grew more rapidly in RAG-1 deficient than sufficient mice suggesting that normal mice make an abortive immune response to this tumor. Depletion of gammadelta T cells or IL-10 augmented the ability of B6 mice to reject E.G7-OVA. Spleen cells from normal, but not IL-10 knockout, mice reconstituted rapid tumor growth in gammadelta T cell-deficient mice. Thus, gammadelta T cells play an important role in preventing immune elimination of this tumor by a mechanism that directly or indirectly involves IL-10.
Departments of Ophthalmology, Pathology and the Winship Cancer Institute, Emory University School of Medicine, Atlanta, GA 30322, USA.
Yong
Y
Ke
Linda M
LM
Kapp
Judith A
JA
Kapp
D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D003433:Crosses, Genetic; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016654:Genes, RAG-1 / Q000276:immunology; D007108:Immune Tolerance / Q000276:immunology; D016753:Interleukin-10 / Q000276:immunology*; D016399:Lymphoma, T-Cell / Q000235:genetics / Q000276:immunology / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010047:Ovalbumin / Q000276:immunology*; D010587:Phagocytosis; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D014162:Transfection; D014407:Tumor Cells, Cultured
D016692:Receptors, Antigen, T-Cell, gamma-delta; D016753:Interleukin-10; D010047:Ovalbumin