Identification and characteristics of nisin Z-producing Lactococcus lactis subsp. lactis isolated from Kimchi.
We isolated bacteriocin-producing Lactococcus lactis subsp. lactis from Kimchi. The bacteriocin inhibited strains of Clostridium perfringens, C. difficile, Listeria monocytogenes, vancomycin-resistant Enterococcus, and one out of four methicillin-resistant Staphylococcus aureus strains, as well as some closely related lactic acid bacteria. In tricine-SDS-PAGE, the bacteriocin migrated with an apparent molecular weight of about 4 kDa to the same location as nisin A and crude nisin Z. The gene encoding this bacteriocin was found to be identical to that of nisin Z with direct PCR sequence methods. The inhibitory activity was stable against heat and pH, but it was lost at 100 degrees C for 1 h and at 121 degrees C for 15 min. The bacteriocin was inactivated by proteolytic enzymes, but was not affected by lysozyme, lipase, catalase, or beta-glucosidase. There were some differences in characteristics from those of nisins described previously.
Laboratory of Veterinary Public Health, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Sang-Hee
SH
Park
Kikuji
K
Itoh
Eisaku
E
Kikuchi
Hidekazu
H
Niwa
Tomohiko
T
Fujisawa
D001419:Bacteria / Q000187:drug effects; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004275:DNA, Ribosomal / Q000032:analysis; D005285:Fermentation; D007723:Korea; D013294:Lactococcus lactis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009561:Nisin / Q000031:analogs & derivatives* / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014675:Vegetables / Q000382:microbiology*
D001426:Bacterial Proteins; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D009561:Nisin; C071364:nisin Z