[Effect of cadmium on apoptosis of spermatogenic cells of rat testis and the protection effect of zinc against it].
To study the effect of cadmium on the apoptosis of spermatogenic cells of rat testis and the protective effect of zinc against it. 24 Wistar male rats were divided into 3 groups (cadmium, cadmium and zinc, and control rats). Rats were injected with low-dose CdCl2 (2 mg/kgBW), zinc acetate(ZnAc, 15 mg/kgBW) before and after injected with low-dose CdCl2 and ZnAc(50 mg/kgBW). The control rats were injected with same-dose of 0.9% NaCl. After 7 days, the apoptosis of spermatogenic cells was studied with in situ nick translation(ISNT) technique. Compared with control group, the number of apoptosis spermategenic cells was obviously increased in cadmium group(P < 0.01, t = 3.87), but it was not significantly different in cadmium and zinc rats. Cadmium treatment could accelerate testis apoptosis. Zinc could proven it.
Li
J
J
Department of Neurobiology, Chinese People's Armed Police Force Medical College, Tianjin 300162, China.
Yi
J
J
Wang
C
C
Xu
P
P
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D019256:Cadmium Chloride / Q000633:toxicity*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013090:Spermatocytes / Q000166:cytology* / Q000187:drug effects; D013737:Testis / Q000166:cytology; D019345:Zinc Acetate / Q000494:pharmacology*
D019345:Zinc Acetate; D019256:Cadmium Chloride