cDNA-AFLP display for the isolation of differentially expressed genes during chicory root development.
To identify genes expressed during root enlargment during the early vegetative growth of chicory (Cichorium intybus L.), we used the cDNA-AFLP technology. The radial pattern of chicory roots was investigated by histological analysis to determine the spatial vascular cambium setting. In young plantlets, serial root sections showed that differentiation of secondary tissues occurred along a gradient extending from the apex to the crown. The cDNA-AFLP technique was carried out on total mRNAs extracted from root tissues producing secondary structures and root tissues developing primary structures only. This study reports on the isolation of two transcript-derived fragments (TDFs) referred to as Y-16 and Y-21. Sequence analysis at the protein level showed that Y-16 carries a sequence highly homologous (93% identities) to the amino acid transporter-like protein 1 (AATL 1) from Arabidopsis and Y-21 presents 72% identity to AAD25141.1 Arabidopsis protein. The expression pattern of both these TDFs was analysed by northern blot and showed an over-expression during early development of chicory roots.
Goupil
Pascale
P
Université des Sciences et Technologies de Lille, Physiologie et Génétique Moléculaire Végétales, Bâtiment SN2, F-59655 Villeneuve d'Ascq, France. Pascale.GOUPIL@ertac.univ-bpclermont.fr
Mahamoud
Yonis Soubere
YS
Poulain
Jérôme
J
Windels
David
D
Crété
Patrice
P
Huss
Brigitte
B
Rambour
Serge
S
D000595:Amino Acid Sequence; D015152:Blotting, Northern; D018651:Cichorium intybus / Q000166:cytology / Q000235:genetics / Q000254:growth & development*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D018517:Plant Roots / Q000166:cytology / Q000235:genetics / Q000254:growth & development*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary