Selenium protection against mercury-induced apoptosis and growth inhibition in cultured K-562 cells.
Selenium and mercuric chloride (MC) interactions regarding effects on cell growth and cell death have been studied. Human K-562 cells were pretreated or simultaneously treated with either selenite (5 or 50 microM) or selenomethionine (10 or 50 microM) and with MC (35 or 50 microM). The 35-microM MC treatments resulted in a clear inhibition of cell growth with no obvious difference between mercury-treated and mercury-selenium-treated cells. Furthermore, the apoptotic frequency was similar at all observations for all selenium treatments with 35 microM MC. In the simultaneously treated selenite and 50- microM MC combinations, a selenite-dependent protection was shown both by increased cell growth and by lower apoptotic frequency at 48 and 96 h of exposure. Both treatments with selenomethionine showed protection observed as an increased cell growth at 48 and 96 h and as decreased apoptotic frequency at 96 h of exposure.
Department of Oncology, Biomedical Radiation Sciences, Rudbeck Laboratory, Uppsala University, Sweden.
Peter
P
Frisk
Kenneth
K
Wester
Amer
A
Yaqob
Ulf
U
Lindh
D017209:Apoptosis / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects*; D006801:Humans; D020287:In Situ Nick-End Labeling; D020014:K562 Cells; D007700:Kinetics; D008627:Mercuric Chloride / Q000037:antagonists & inhibitors / Q000633:toxicity*; D012645:Selenomethionine / Q000494:pharmacology*; D018038:Sodium Selenite / Q000494:pharmacology*; D013997:Time Factors
D008627:Mercuric Chloride; D012645:Selenomethionine; D018038:Sodium Selenite