Characterization of a novel coronavirus associated with severe acute respiratory syndrome.
In March 2003, a novel coronavirus (SARS-CoV) was discovered in association with cases of severe acute respiratory syndrome (SARS). The sequence of the complete genome of SARS-CoV was determined, and the initial characterization of the viral genome is presented in this report. The genome of SARS-CoV is 29,727 nucleotides in length and has 11 open reading frames, and its genome organization is similar to that of other coronaviruses. Phylogenetic analyses and sequence comparisons showed that SARS-CoV is not closely related to any of the previously characterized coronaviruses.
Rota
Paul A
PA
National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA. prota@cdc.gov
Oberste
M Steven
MS
Monroe
Stephan S
SS
Nix
W Allan
WA
Campagnoli
Ray
R
Icenogle
Joseph P
JP
Peñaranda
Silvia
S
Bankamp
Bettina
B
Maher
Kaija
K
Chen
Min-Hsin
MH
Tong
Suxiong
S
Tamin
Azaibi
A
Lowe
Luis
L
Frace
Michael
M
DeRisi
Joseph L
JL
Chen
Qi
Q
Wang
David
D
Erdman
Dean D
DD
Peret
Teresa C T
TC
Burns
Cara
C
Ksiazek
Thomas G
TG
Rollin
Pierre E
PE
Sanchez
Anthony
A
Liffick
Stephanie
S
Holloway
Brian
B
Limor
Josef
J
McCaustland
Karen
K
Olsen-Rasmussen
Melissa
M
Fouchier
Ron
R
Günther
Stephan
S
Osterhaus
Albert D M E
AD
Drosten
Christian
C
Pallansch
Mark A
MA
Anderson
Larry J
LJ
Bellini
William J
WJ
D000595:Amino Acid Sequence; D017124:Conserved Sequence; D017934:Coronavirus / Q000145:classification / Q000235:genetics; D000086862:Coronavirus M Proteins; D000086462:Coronavirus Nucleocapsid Proteins; D018076:DNA, Complementary; D010450:Endopeptidases / Q000737:chemistry / Q000235:genetics; D016679:Genome, Viral; D006801:Humans; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins / Q000737:chemistry / Q000235:genetics; D016366:Open Reading Frames; D010802:Phylogeny; D020815:Polyproteins / Q000737:chemistry / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase / Q000737:chemistry / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D045473:Severe acute respiratory syndrome-related coronavirus / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D045169:Severe Acute Respiratory Syndrome / Q000821:virology; D064370:Spike Glycoprotein, Coronavirus; D014158:Transcription, Genetic; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics; D014763:Viral Matrix Proteins / Q000737:chemistry / Q000235:genetics; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*
D000086862:Coronavirus M Proteins; D000086462:Coronavirus Nucleocapsid Proteins; D018076:DNA, Complementary; C000706237:M protein, SARS-CoV; D008562:Membrane Glycoproteins; D019590:Nucleocapsid Proteins; D020815:Polyproteins; D012333:RNA, Messenger; D012367:RNA, Viral; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins; D014763:Viral Matrix Proteins; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase; D010450:Endopeptidases