Metabolic engineering to produce sesquiterpenes in yeast.
[reaction: see text] Presented here is a metabolically engineered yeast strain that produces sesquiterpenes. Epi-cedrol synthase expressed in a native yeast strain converted endogenous farnesyl pyrophosphate to 90 microg/L epi-cedrol. This system was genetically modified to increase foreign terpene yields to 370 microg/L. The best yields were obtained by overexpressing a truncated 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase in a upc2-1 mating type a background. This system allows sufficient production to characterize novel sesquiterpene synthase genes.
Jackson
Beth E
BE
Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77005, USA.
Hart-Wells
Elizabeth A
EA
Matsuda
Seiichi P T
SP
D019755:Carbon-Carbon Lyases / Q000378:metabolism; D003500:Cyclization; D005818:Genetic Engineering; D006903:Hydroxymethylglutaryl CoA Reductases / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D012717:Sesquiterpenes / Q000138:chemical synthesis*
D012717:Sesquiterpenes; D006903:Hydroxymethylglutaryl CoA Reductases; D019755:Carbon-Carbon Lyases; C122071:8-epicedrol synthase