Induction of short interspersed nuclear repeat-containing transcripts in epithelial cells upon infection with a chicken adenovirus.
Chicken embryo lethal orphan adenovirus (CELO) is used as a vector for expression of exogenous genes in mammalian cells. Here, we analyzed transcriptional alterations in mouse epithelial host cells following infection with CELO using cDNA microarray analysis. Sequence data characterization revealed that a major portion of CELO-induced genes contained short interspersed nuclear elements of the B2 subclass (B2 SINEs). In fact, we could identify SINEs and other repetitive sequences as contributing significantly to the cDNAs used for microarray construction. Moreover, we found that the CELO protein Gam1 was able to mediate transcriptional activation of these B2 SINE-containing RNAs. We hypothesize that upregulation of B2-SINE-containing RNAs could be a novel contribution of Gam1 to CELO host cell infection.
Wick
Nikolaus
N
Department of Histology and Molecular Cell Biology, Institute for Anatomy, Histology and Embryology, University of Innsbruck, Austria, Austria.
Luedemann
Susanna
S
Vietor
Ilja
I
Cotten
Matt
M
Wildpaner
Michael
M
Schneider
Georg
G
Eisenhaber
Frank
F
Huber
Lukas A
LA
D000818:Animals; D000259:Aviadenovirus / Q000235:genetics*; D001483:Base Sequence; D002642:Chick Embryo; D018076:DNA, Complementary / Q000378:metabolism; D019992:Databases as Topic; D004847:Epithelial Cells / Q000378:metabolism / Q000821:virology*; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D016679:Genome, Viral; D051379:Mice; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D020543:Proteome; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020086:Short Interspersed Nucleotide Elements / Q000235:genetics*; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000096:biosynthesis
C105052:CELO protein, adenovirus; D018076:DNA, Complementary; D020543:Proteome; D012333:RNA, Messenger; D014764:Viral Proteins