Structural alterations of the vascular wall in magnesium-deficient mice. A possible role of gelatinases A (MMP-2) and B (MMP-9).
Vascular alterations during magnesium deficiency have long been known but the implicated mechanisms have so far been poorly documented. In this preliminary assay, we compared the thoracic aortic histology in Swiss OF1 mice fed a severe magnesium-deficient diet (50 +/- 5 ppm) for 42 days to that of controls fed a standard diet (1700 +/- 100 ppm magnesium). It appeared (eosin-haematoxylin coloration) that, in magnesium-deficient mice, the aortic wall was thinner than in controls. Specific colorations of the two of main fibers vascular tissue (collagens and elastin) showed severe structural alterations of both components. These changes were consecutive to the expression of matrix metalloproteinases (MMP) -2 and -9 which were present as zymogens (inactive forms) in controls and supposed to be present in their active and inactive forms in magnesium-deficient mice (zymography). These changes which have not been reported so far would explain, at least in part, the sensitivity of magnesium-deficient mice to various stress or xenobiotics.
Faculté de Pharmacie, Route du Rhin, Strasbourg, 76400 Illkirch, France. nicole.pages4@wanadoo.fr
N
N
Pagès
B
B
Gogly
G
G
Godeau
S
S
Igondjo-Tchen
P
P
Maurois
J
J
Durlach
P
P
Bac
D000818:Animals; D001013:Aorta, Thoracic / Q000201:enzymology / Q000473:pathology; D002417:Cattle; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000201:enzymology* / Q000473:pathology*; D008274:Magnesium / Q000097:blood; D008275:Magnesium Deficiency / Q000201:enzymology* / Q000473:pathology; D020778:Matrix Metalloproteinase 2 / Q000502:physiology*; D020780:Matrix Metalloproteinase 9 / Q000502:physiology*; D051379:Mice; D013552:Swine
D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D008274:Magnesium