Micro-X-ray fluorescence as a general high-throughput screening method for catalyst discovery and small molecule recognition.
A powerful high-throughput screening technique is described for the rapid screening of bead-based libraries for catalyst discovery and molecular recognition. Micro-X-ray fluorescence (MXRF) screens materials for elemental composition with mesoscale analysis. This method is nondestructive and requires minimal sample preparation and no special tags for analysis, and the screening time is dependent on the desired sensitivity. The speed, sensitivity, and simplicity of MXRF as a high-throughput screening technique were applied to screen bead-based libraries of oligopeptides for phosphate hydrolysis catalysts and molecular recognition of selective receptors for the degradation products and analogues of chemical warfare agents. This paper demonstrates the analytical or HTS capability of MXRF for combinatorial screening. It is meant only to show the capabilities of MXRF and is not meant as an exhaustive study of the catalyst and molecular recognition systems presented.
Miller
Thomasin C
TC
Los Alamos National Laboratory, Chemistry Division, Mail Stop K484, Los Alamos, New Mexico 87545, USA.
Mann
Grace
G
Havrilla
George J
GJ
Wells
Cyndi A
CA
Warner
Benjamin P
BP
Baker
R Tom
RT
D002384:Catalysis; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005453:Fluorescence; D006868:Hydrolysis; D018377:Neurotransmitter Agents / Q000737:chemistry / Q000378:metabolism; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism*; D019151:Peptide Library; D010710:Phosphates / Q000378:metabolism; D012680:Sensitivity and Specificity; D013997:Time Factors; D014965:X-Rays
D018377:Neurotransmitter Agents; D009842:Oligopeptides; D019151:Peptide Library; D010710:Phosphates