Effect of antisense oligonucleotide of noggin on spatial learning and memory of rats.
OBJECTIVE
To investigate the effect of antisense oligonucleotide (ASODN) of noggin on rat spatial learning and memory.


METHODS
Expression of noggin mRNA was measured by in situ hybridization method and the ability to spatial learning and memory was tested with Morris water maze.


RESULTS
Compared with control rats, noggin mRNA positive neurons in dentate gyrus (DG) and CA3 region of hippocampus were markedly increased after the Morris water maze training (P<0.01). The increase of noggin mRNA positive neurons in hippocampus following maze training could be significantly blocked by icv injection of antisense noggin ODN, and the injection also impaired the learning and memory formation as compared to that in control rats. But the sense oligonucleotide (SODN) had no effect.


CONCLUSIONS
Noggin, as an embryonic gene expressed in adult hippocampus, plays an important role in the process of learning and memory formation.
Department of Neurobiology, Third Military Medical University, Chongqing 400038, China.
Xiao-Tang
XT
Fan
Wen-Qin
WQ
Cai
Zhong
Z
Yang
Hai-Wei
HW
Xu
Jin-Hai
JH
Zhang
D000818:Animals; D002352:Carrier Proteins; D005260:Female; D006624:Hippocampus / Q000187:drug effects / Q000502:physiology; D008297:Male; D018782:Maze Learning / Q000187:drug effects*; D008568:Memory / Q000187:drug effects*; D009474:Neurons / Q000378:metabolism; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D011506:Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013028:Space Perception / Q000187:drug effects; D000099176:Noggin Protein
D002352:Carrier Proteins; D016376:Oligonucleotides, Antisense; D011506:Proteins; D012333:RNA, Messenger; D000099176:Noggin Protein