Mouse Aortic Ring Assay: A New Approach of the Molecular Genetics of Angiogenesis.
Angiogenesis, a key step in many physiological and pathological processes, involves proteolysis of the extracellular matrix. To study the role of two enzymatic families, serine-proteases and matrix metalloproteases in angiogenesis, we have adapted to the mouse, the aortic ring assay initially developed in the rat. The use of deficient mice allowed us to demonstrate that PAI-1 is essential for angiogenesis while the absence of an MMP, MMP-11, did not affect vessel sprouting. We report here that this model is attractive to elucidate the cellular and molecular mechanisms of angiogenesis, to identify, characterise or screen "pro- or anti-angiogenic agents that could be used for the treatment of angiogenesis-dependent diseases. Approaches include using recombinant proteins, synthetic molecules and adenovirus-mediated gene transfer.
Masson V
V éRonique
Laboratory of Tumor and Developmental Biology, Université de Liège. Tour de Pathologie (B23), Sart Tilman, B-4000 Liège. Belgium.; Center for Transgene Technology and Gene Therapy, Flanders Interuniversity Institute for Biotechnology, Katholieke Universiteit Leuven. B-3000 Leuven. Belgium.Rhone-Poulenc Rorer. Hayward, California, 94545. USA. agnes.noel@ulg.ac.be
Devy
Laetitia
L
Grignet-Debrus
Christine
C
Bernt
Sarah
S
Bajou
Khalid
K
Blacher
Silvia
S
Roland
Guy
G
Chang
Yawen
Y
Fong
Timothy
T
Carmeliet
Peter
P
Foidart
Jean-Michel
JM
Noël
Agnès
A