Sinorhizobium americanus sp. nov., a new Sinorhizobium species nodulating native Acacia spp. in Mexico.
The sinorhizobia isolated from root nodules of Acacia species native of Mexico constitute a diverse group of bacteria on the basis of their metabolic enzyme electromorphs but share restriction patterns of the PCR products of 16S rRNA genes and a common 500 kb symbiotic plasmid. They are distinguished from other Sinorhizobium species by their levels of DNA-DNA hybridization and the sequence of 16S rRNA and nifH genes. nolR gene hybridization patterns were found useful to identify sinorhizobia and characterize species. A new species, Sinorhizobium americanus, is described and the type strain is CFNEI 156 from Acacia acatlensis.
Centro de Investigación sobre Fijación de Nitrógeno, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México.
Ivonne
I
Toledo
Lourdes
L
Lloret
Esperanza
E
Martínez-Romero
D000045:Acacia / Q000145:classification / Q000382:microbiology*; D001483:Base Sequence; D024901:Drug Resistance, Multiple, Bacterial; D004798:Enzymes / Q000032:analysis / Q000145:classification / Q000378:metabolism; D007612:Kanamycin / Q000494:pharmacology; D008800:Mexico; D009268:Nalidixic Acid / Q000494:pharmacology; D009586:Nitrogen Fixation; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D020579:Sinorhizobium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure; D012988:Soil Microbiology
D004798:Enzymes; D012336:RNA, Ribosomal, 16S; D009268:Nalidixic Acid; D007612:Kanamycin