[A method for preparation of synaptonemal complexes of meiotic chromosomes from basidial protoplasts of the common mushroom Agaricus bisporus (Lange) Imbach].
For the first time, preparations of synaptonemal complexes (SCs) were made from meiotic chromosomes of white button mushroom (Agaricus bisporus) basidia. It is the first experience of obtaining SC preparations of filamentous fungi from isolated meiosporangium protoplasts. Previously, only yeast SC preparations were obtained following this approach. The method includes four major stages: isolation of basidium protoplasts by treatment of basidia with lytic enzymes, spreading of protoplast nuclei on a filmy support by osmotic shock, staining the preparations with silver nitrate, and examination under light and electron microscopes. The structures of spread premeiotic nuclei, axial elements of chromosomes, SCs, chromatin, and nucleoli were studied at the leptotene-diplotene stage of meiotic prophase I.
Department of Mycology and Algology, Lomonosov Moscow State University, Moscow, 119899 Russia.
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Mazheĭka
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Kolomiets
D000364:Agaricus / Q000235:genetics*; D015825:Chromosomes, Fungal / Q000737:chemistry*; D003902:Detergents / Q000737:chemistry; D004492:Edetic Acid / Q000737:chemistry; D008540:Meiosis; D008967:Molecular Biology / Q000379:methods*; D011523:Protoplasts / Q000502:physiology* / Q000648:ultrastructure; D013347:Subcellular Fractions / Q000737:chemistry; D013573:Synaptonemal Complex / Q000737:chemistry* / Q000648:ultrastructure
D003902:Detergents; D004492:Edetic Acid