Expression of placental low-density lipoprotein receptor-related protein and scavenger receptors AI/AII transcripts in the baboon.
OBJECTIVE
This study was undertaken to assess potential secondary lipoprotein-dependent mechanisms, scavenger receptor AI and AII (SR-AI and SR-AII), and low-density lipoprotein (LDL) receptor-related protein (LRP) in the baboon (Papio sp) placenta.


METHODS
Baboon placental villous tissue and enriched fractions of syncytiotrophoblast cells were collected at early (approximately day 60, n = 3), mid (approximately day 100, n = 4), and late (approximately day 160, n = 4) pregnancy (term = approximately 184 days). The abundance of messenger RNA (mRNA) transcripts for SR-AI, SR-AII, and LRP were determined relative to constitutively expressed, glyceraldehyde-3-phosphate-dehydrogenase (GAPDH).


RESULTS
No quantitative differences (P >.05) in SR-AI, SR-AII, or LRP mRNAs were noted in respect to stage of gestation. However, SR-AII/GAPDH mRNA ratios in syncytiotrophoblast cells (0.38 +/- 0.09, n = 11) were significantly (P <.05) higher than in villous tissue (0.15 +/- 0.04, n = 11), whereas LRP/GAPDH mRNA ratios were higher in villous tissue (0.43 +/- 0.10, n = 11) than in syncytiotrophoblast cells (0.18 +/- 0.02, n = 11).


CONCLUSIONS
SR-AII mRNA in baboon placenta appears to be expressed, in greatest abundance, in the endocrinologically active syncytiotrophoblast.
Shi
Wenliang
W
Department of Obstetrics and Gynecology, Tulane University Health Sciences Center, 1430 Tulane Avenue, New Orleans, LA 70112-2699, USA.
Swan
Kenneth F
KF
Henson
Michael C
MC
D000818:Animals; D018955:CD36 Antigens / Q000235:genetics / Q000378:metabolism*; D002824:Chorionic Villi / Q000378:metabolism*; D005260:Female; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D026563:LDL-Receptor Related Protein-Associated Protein / Q000235:genetics / Q000378:metabolism*; D010215:Papio; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D051117:Scavenger Receptors, Class A; D014327:Trophoblasts / Q000378:metabolism*
D018955:CD36 Antigens; D026563:LDL-Receptor Related Protein-Associated Protein; D012333:RNA, Messenger; D051117:Scavenger Receptors, Class A; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases