Anti-metastatic effects of aqueous extract of Helixanthera parasitica.
Metastasis, the spread of cancer in body, is a major cause of death. We have screened anti-metastatic activity of aqueous and dichloromethane extracts of several not previously studied Thai herbs, using an in vitro invasion test. This involves the in vitro invasion of HCC-S102, a hepatocellular carcinoma cell line derived from a Thai patient, through a reconstituted-basement membrane (Matrigel). The aqueous extract of a plant (Helixanthera parasitica) revealed a significant inhibitory effect on the cancer cell invasion, and showed antioxidant activity. The aqueous extract was partially purified by silica gel column chromatography, and the highest anti-metastatic activity fraction showed 83% inhibition of invasion with low cytotoxic effect. However, anti-metastatic activity was not associated with the antioxidant activity of the aqueous extract.
Laboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210, Thailand.
Kriengsak
K
Lirdprapamongkol
Chulabhorn
C
Mahidol
Sanit
S
Thongnest
Hunsa
H
Prawat
Somsak
S
Ruchirawat
Chantragan
C
Srisomsap
Rudee
R
Surarit
Phaibul
P
Punyarit
Jisnuson
J
Svasti
D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification* / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy*; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy*; D010936:Plant Extracts / Q000302:isolation & purification* / Q000627:therapeutic use; D010946:Plants, Medicinal; D013785:Thailand; D014407:Tumor Cells, Cultured / Q000187:drug effects*
D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts