Ovarian stimulation of marmoset monkeys (Callithrix jacchus) using recombinant human follicle stimulating hormone.
To reduce the number of animals required for controlled studies of marmoset oocytes and early embryos, a superovulation protocol was developed for the common marmoset. Females were given up to 50 i.u./day recombinant human follicle stimulating hormone (FSH)--(r-hFSH) for 6 days. Ovaries were visualized by a modified laparoscopic technique and follicular aspiration was performed using a needle and suction apparatus inserted directly through an otoscope speculum. The number of follicles + ovulation points (+/- S.E.) was 2.9 (+/- 0.2) in controls and 14.1 (+/- 1.6; P < or = 0.001) in the 50 i.u. r-hFSH per day animals. Oocytes, typically at the germinal vesicle stage at collection, extruded a first polar body within 26 hours. In vitro fertilization was performed and embryos developed to the hatched blastocyst stage (34%). With many high quality oocytes and the ability to synchronize cycles, the marmoset is a valuable primate model for examining nuclear reprograming and early embryonic events.
Marshall
V S
VS
Wisconsin Primate Research Center, University of Wisconsin Medical School, University of Wisconsin, Madison, WI 53715, USA.
Browne
M A
MA
Knowles
L
L
Golos
T G
TG
Thomson
J A
JA
D000818:Animals; D001755:Blastocyst / Q000187:drug effects; D002144:Callithrix / Q000502:physiology*; D004622:Embryo, Mammalian / Q000187:drug effects; D005260:Female; D005307:Fertilization in Vitro; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D006801:Humans; D008297:Male; D009865:Oocytes / Q000187:drug effects; D010053:Ovary / Q000187:drug effects*; D011994:Recombinant Proteins / Q000494:pharmacology; D013480:Superovulation / Q000187:drug effects
D011994:Recombinant Proteins; D005640:Follicle Stimulating Hormone