ICAM-1 can play a major role in mediating P. falciparum adhesion to endothelium under flow.
We have investigated the importance of adhesion molecule co-operation in mediating Plasmodium falciparum adhesion to endothelial cells under flow conditions. Using three laboratory parasite lines and a patient isolate which differ in their ICAM-1 and CD36-binding avidity, we found that blockade of ICAM-1 and CD36 separately reduced IRBC adhesion by up to 95 and 50%, respectively. These results confirm previous data showing that ICAM-1 and CD36 synergize to mediate adhesion, but differ in demonstrating that without ICAM-1, binding under flow conditions is severely impaired. Thus, in this system, ICAM-1 is critical for P. falciparum adhesion to activated endothelium and once bound, synergy with CD36 mediates the majority (> or =98%) of adhesion.
Molecular Biochemistry and Parasitology, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, UK.
Carolyn
C
Gray
Christopher
C
McCormick
Gareth
G
Turner
Alister
A
Craig
D000818:Animals; D001665:Binding Sites; D018955:CD36 Antigens / Q000378:metabolism; D002448:Cell Adhesion; D020405:Dermis / Q000033:anatomy & histology / Q000166:cytology; D004730:Endothelium, Vascular / Q000166:cytology / Q000469:parasitology* / Q000502:physiology; D006439:Hemodynamics; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000502:physiology*; D016778:Malaria, Falciparum / Q000469:parasitology*; D010963:Plasmodium falciparum / Q000378:metabolism / Q000472:pathogenicity*; D014470:Umbilical Cord / Q000033:anatomy & histology / Q000166:cytology
D018955:CD36 Antigens; D018799:Intercellular Adhesion Molecule-1