Moderately lipophilic carboxylate compounds are the selective inducers of the Saccharomyces cerevisiae Pdr12p ATP-binding cassette transporter.
Saccharomyces cerevisiae displays very strong induction of a single ATP-binding cassette (ABC) transporter, Pdr12p, when stressed with certain weak organic acids. This is a plasma membrane pump catalysing active efflux of the organic acid anion from the cell. Pdr12p action probably allows S. cerevisiae to maintain lower intracellular levels of several weak organic acid preservatives than would be expected on the basis of the free equilibration of the acid across the cell membrane. This in turn facilitates growth in the presence of these preservatives and therefore yeast spoilage of food materials. Pdr12p appears to confer resistance to those carboxylic acids that, to a reasonable degree, partition into both the lipid bilayer and aqueous phases. Its gene (PDR12) is strongly induced by sorbate, benzoate and certain other moderately lipophilic carboxylate compounds, but not by organic alcohols or high levels of acetate. PDR12 induction reflects the operation of a previously uncharacterized S. cerevisiae stress response, for which the induction signal is probably a high intracellular pool of the organic acid anion.
Hatzixanthis
Kostas
K
Department of Biochemistry and Molecular Biology, University College London, London WC1E 6BT, UK.
Mollapour
Mehdi
M
Seymour
Ian
I
Bauer
Bettina E
BE
Krapf
Gerd
G
Schüller
Christoph
C
Kuchler
Karl
K
Piper
Peter W
PW
D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis* / Q000235:genetics; D001483:Base Sequence; D020013:Calcium Signaling; D002264:Carboxylic Acids / Q000737:chemistry / Q000494:pharmacology*; D004271:DNA, Fungal / Q000235:genetics; D005516:Food Microbiology; D005520:Food Preservatives / Q000494:pharmacology; D017930:Genes, Reporter; D050890:HSP30 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000235:genetics; D007763:Lac Operon; D008565:Membrane Proteins / Q000235:genetics; D009997:Osmotic Pressure; D018384:Oxidative Stress; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis* / Q000235:genetics
D018528:ATP-Binding Cassette Transporters; D002264:Carboxylic Acids; D004271:DNA, Fungal; D005520:Food Preservatives; D050890:HSP30 Heat-Shock Proteins; C494390:HSP30 protein, S cerevisiae; D006360:Heat-Shock Proteins; D008565:Membrane Proteins; C472673:Pdr12 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins