Isolation of Paenibacillus illinoisensis that produces cyclodextrin glucanotransferase resistant to organic solvents.
A bacterium that secreted cyclodextrin glucanotransferase (CGTase) in a medium overlaid with n-hexane was isolated and identified as Paenibacillus illinoisensis strain ST-12 K. The CGTase of the strain was purified from the culture supernatant. The molecular mass was 70 kDa. The enzyme was stable at pH 6 to 10 and active at pH 5.0 to 8.0. The optimum temperature at pH 7.0 was 65 degrees C in the presence of 5 mM CaCl2. The enzyme produced mainly beta-cyclodextrin. The total yield of alpha-, beta-, and gamma- cyclodextrins was increased 1.4-fold by the addition of ethanol. In particular, the yield of beta-cyclodextrins in the presence of 10% (vol/vol) ethanol was 1.6-fold that without ethanol. The CGTase was stable and active in the presence of large amounts of various organic solvents.
Doukyu
Noriyuki
N
Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta 4259, Midori-ku, Yokohama 226-8501, Japan. ndoukyu@bio.titech.ac.jp
Kuwahara
Hirokazu
H
Aono
Rikizo
R
D001407:Bacillus / Q000145:classification / Q000201:enzymology / Q000302:isolation & purification*; D003505:Cyclodextrins / Q000032:analysis / Q000096:biosynthesis; D004795:Enzyme Stability; D005964:Glucosyltransferases / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009930:Organic Chemicals; D012997:Solvents; D013696:Temperature
D003505:Cyclodextrins; D009930:Organic Chemicals; D012997:Solvents; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase