Amperometric detection of perphenazine at a carbon fiber micro-disk bundle electrode by capillary zone electrophoresis.
A simple method for determination of perphenazine by capillary zone electrophoresis with amperometric detection is described. The optimum conditions of separation and detection are 1.50 x 10(-3) mol/l Na(2)B(4)O(7)-1.0 x 10(-3) mol/l NaOH (pH 9.9) for the buffer solution, 18 kV for the separation voltage, 5 kV and 5 s for the injection voltage and the injection time, and 0.80 V versus saturated calomel electrode for the detection potential, respectively. The limit of detection is 5.0 x 10(-8) mol/l or 44 amol (S/N=3). The linear range of the calibration curve is 1.00 x 10(-7) to 1.00 x 10(-4) mol/l. The relative standard deviation is 1.5% for the migration time and 2.9% for the electrophoretic current at peak maximum. The method is applied to the determination of perphenazine in human urine.
School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China.
Daxing
D
Liu
Wenrui
W
Jin
D014150:Antipsychotic Agents / Q000652:urine*; D002138:Calibration; D002244:Carbon / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D010546:Perphenazine / Q000652:urine*
D014150:Antipsychotic Agents; D002244:Carbon; D010546:Perphenazine