Light-induced electron transfer in a cryptochrome blue-light photoreceptor.
Cryptochromes are flavoproteins implicated in multiple blue light-dependent signaling pathways regulating, for example, photomorphogenesis in plants or circadian clocks in animals. Using transient absorption spectroscopy, it is demonstrated that the primary light reactions in isolated Arabidopsis thaliana cryptochrome-1 involve intraprotein electron transfer from tryptophan and tyrosine residues to the excited flavin adenine dinucleotide cofactor.
Université Paris VI, Tour 53 E 5, Casier 156, 4, Place Jussieu, 75252 Paris Cedex 05, France.
Baldissera
B
Giovani
Martin
M
Byrdin
Margaret
M
Ahmad
Klaus
K
Brettel
D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D056931:Cryptochromes; D029721:Drosophila Proteins; D004579:Electron Transport / Q000502:physiology / Q000528:radiation effects; D005136:Eye Proteins; D005182:Flavin-Adenine Dinucleotide / Q000737:chemistry / Q000378:metabolism; D005420:Flavoproteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D008027:Light; D017956:Photoreceptor Cells, Invertebrate; D043562:Receptors, G-Protein-Coupled; D013057:Spectrum Analysis / Q000379:methods; D014364:Tryptophan / Q000737:chemistry / Q000378:metabolism; D014443:Tyrosine / Q000737:chemistry / Q000378:metabolism
D029681:Arabidopsis Proteins; D056931:Cryptochromes; D029721:Drosophila Proteins; D005136:Eye Proteins; D005420:Flavoproteins; D043562:Receptors, G-Protein-Coupled; C119828:cry protein, Drosophila; D005182:Flavin-Adenine Dinucleotide; D014443:Tyrosine; D014364:Tryptophan