Structural and serological studies of the O-antigen of Proteus mirabilis O-9.
The following structure of the O-polysaccharide (O-antigen) of the lipopolysaccharide of Proteus mirabilis O-9 was determined by NMR spectroscopy, including 2D 1H,(1)H COSY, TOCSY, ROESY, and 1H,(13)C HMQC experiments, along with chemical methods: [chemical structure: see text] where the degree of O-acetylation is approximately 70%. Immunochemical studies using rabbit polyclonal anti-Proteus mirabilis O-9 serum showed the importance of the O-acetyl groups in manifesting the serological specificity of the O-9 antigen. Anti-P. mirabilis O-9 cross-reacted with the lipopolysaccharides (LPS) of P. vulgaris O-25 and Proteus penneri 14, which could be accounted for by a structural similarity of their O-polysaccharides.
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russia.
Anna N
AN
Kondakova
Rafal
R
Fudala
Sof'ya N
SN
Senchenkova
Aleksander S
AS
Shashkov
Yuriy A
YA
Knirel
Wieslaw
W
Kaca
D000107:Acetylation; D000818:Animals; D002240:Carbohydrate Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D007106:Immune Sera / Q000276:immunology*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000032:analysis*; D011513:Proteus mirabilis / Q000145:classification / Q000276:immunology*; D011817:Rabbits; D012703:Serotyping
D007106:Immune Sera; D019081:O Antigens