[Reversed-phase high performance liquid chromatography of the products of microbiological degradation of toluene ].
Conditions have been selected for a reversed-phase high-performance liquid chromatographic assay of intermediate products formed in the course of utilization of toluene by Pseudomonas putida. The composition of products indicates that degradation of toluene by strain BS590-P proceeds primarily through the formation of benzoate and catechol. This is followed by degradation of catechol via ortho-cleavage. In strain BS3701-P, toluene oxidation involves both the side chain and the aromatic ring.
Zelenkova
N F
NF
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, prosp. Nauki 5, Pushchino, Moscow Oblast, 142290 Russia. zelen@ibpm.serpukhov.su
Arinbasarov
M U
MU
D001565:Benzoates / Q000032:analysis / Q000378:metabolism; D019817:Benzoic Acid / Q000032:analysis / Q000378:metabolism; D002396:Catechols / Q000032:analysis / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D010084:Oxidation-Reduction; D016958:Pseudomonas putida / Q000378:metabolism*; D014050:Toluene / Q000378:metabolism*
D001565:Benzoates; D002396:Catechols; D014050:Toluene; D019817:Benzoic Acid; C034221:catechol