Amomum xanthoides extract prevents cytokine-induced cell death of RINm5F cells through the inhibition of nitric oxide formation.
We previously showed that Amomum xanthoides extract prevented alloxan-induced diabetes through the suppression of NF-kappaB activation. In this study, the preventive effects of A. xanthoides extract on cytokine-induced beta-cell destruction were examined. Cytokines produced by immune cells infiltrating pancreatic islets are important mediators of beta-cell destruction in insulin-dependent diabetes mellitus. A. xanthoides extract completely protected interleukin-1beta (IL-1beta) and interferon-gamma (IFN-gamma)-mediated cytotoxicity in rat insulinoma cell line (RINm5F). Incubation with A. xanthoides extract resulted in a significant reduction in IL-1beta and IFN-gamma-induced nitric oxide (NO) production, a finding that correlated well with reduced levels of the inducible form of NO synthase (iNOS) mRNA and protein. The molecular mechanism by which A. xanthoides extract inhibited iNOS gene expression appeared to involve the inhibition of NF-kappaB activation. Our results revealed the possible therapeutic value of A. xanthoides extract for the prevention of diabetes mellitus progression.
Kwon
Kang-Beom
KB
Department of Physiology, School of Oriental Medicine, Won-Kwang University, Iksan, Chonbuk 570-749, South Korea.
Kim
Jin-Hong
JH
Lee
Young-Rae
YR
Lee
Hak-Yong
HY
Jeong
Yeon-Jun
YJ
Rho
Hye-Won
HW
Ryu
Do-Gon
DG
Park
Jin-Woo
JW
Park
Byung-Hyun
BH
D035801:Amomum; D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D016207:Cytokines / Q000494:pharmacology*; D003920:Diabetes Mellitus / Q000188:drug therapy; D007340:Insulinoma / Q000473:pathology; D007371:Interferon-gamma / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology; D007515:Islets of Langerhans / Q000187:drug effects* / Q000473:pathology; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D019001:Nitric Oxide Synthase / Q000235:genetics; D052247:Nitric Oxide Synthase Type II; D010936:Plant Extracts / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats
D016207:Cytokines; D007375:Interleukin-1; D010936:Plant Extracts; D012333:RNA, Messenger; D009569:Nitric Oxide; D007371:Interferon-gamma; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat