Cytotoxicity of antimicrobial tissue conditioners containing silver-zeolite.
OBJECTIVE
The purpose of this study was to determine the effects of incorporating antimicrobial silver-zeolite on the in vitro cytotoxicities of five tissue conditioners against the living dermal model, which consisted of normal human dermal fibroblasts in a collagen lattice.


METHODS
Samples of each tissue conditioner containing silver-zeolite (2 wt% and 5 wt%) were prepared; samples without silver-zeolite were used as a control. Cytotoxicity of each sample against the living dermal model was evaluated by cell viability with MTT assay.


RESULTS
Cell viabilities for Visco-Gel, GC Soft-Liner, FITT, and SR-Ivoseal decreased with increasing silver-zeolite content. Cell viability for Shofu Tissue Conditioner showed no significant difference with degree of silver-zeolite incorporation and had higher values than other tissue conditioners.


CONCLUSIONS
Individual compounds eluted from tissue conditioners and silver ions released from silver-zeolite might cause such cytotoxicity. The results suggest that Shofu Tissue Conditioner is a possible candidate for a novel antimicrobial tissue conditioner containing silver-zeolite because it showed the highest cell viability and the smallest influence of incorporating silver-zeolite.
Department of Advanced Prosthodontics, Division of Cervico-Gnathostomatology, Programs for Applied Biomedicine, Graduate School of Biomedical Sciences, Hiroshima University, Japan. abey@hiroshima-u.ac.jp
Yasuhiko
Y
Abe
Morikatsu
M
Ueshige
Maho
M
Takeuchi
Mie
M
Ishii
Yasumasa
Y
Akagawa
D000180:Acrylic Resins / Q000633:toxicity; D000704:Analysis of Variance; D000890:Anti-Infective Agents / Q000633:toxicity; D018929:Cell Culture Techniques; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents; D003764:Dental Materials / Q000633:toxicity*; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D008689:Methacrylates / Q000633:toxicity; D008768:Methylmethacrylates / Q000633:toxicity; D010795:Phthalic Acids / Q000633:toxicity; D012834:Silver / Q000633:toxicity*; D012867:Skin / Q000166:cytology / Q000187:drug effects; D013778:Tetrazolium Salts; D013844:Thiazoles; D014016:Tissue Conditioning, Dental; D017641:Zeolites / Q000633:toxicity*
D000180:Acrylic Resins; D000890:Anti-Infective Agents; D004396:Coloring Agents; D003764:Dental Materials; D008689:Methacrylates; D008768:Methylmethacrylates; D010795:Phthalic Acids; D013778:Tetrazolium Salts; D013844:Thiazoles; C073909:GC Soft-Liner; D017641:Zeolites; C043685:Coe Comfort; D012834:Silver; C009931:poly(ethylmethacrylate); C022616:thiazolyl blue