Fermentation conditions and properties of a chitosanase from Acinetobacter sp. C-17.
A species of bacterium with high chitosanase activity was isolated from soil samples in Haiyan City, China, and identified as an Acinetobacter species. This strain, named Acinetobacter sp. strain C-17, produced a chitosanase that was inducible and secreted into the medium. The optimal conditions for enzyme production were cells used to inoculate a medium containing 1% chitosan (pH 7.0) followed by culture at 30 degrees C. The chitosanase activity reached 1.7 U/ml when strain C-17 was incubated in a 250-ml flask under the optimal conditions for 24 h, and reached 2.8 U/ml when cells were incubated in a 3-l fermentor. The optimal pH and temperature for hydrolysis of chitosanase were 7.0 and 36 degrees C, respectively. The chitosanase activity was stable in the pH range of 5-8 and temperature range of 30-40 degrees C. The chitosanase of the strain was extracted by zinc acetate and ammonium sulfate precipitation. The molecular mass was estimated to be 35.4 kDa by SDS-PAGE.
Zhu
Xu-Fen
XF
College of Life Science, Zhejiang University, Hangzhou, Zhejiang 310012, People's Republic of China. zhuxufen@mail.hz.zj.cn
Wu
Xiao-Yun
XY
Dai
Yun
Y
D000150:Acinetobacter / Q000145:classification / Q000201:enzymology*; D011232:Chemical Precipitation; D002686:Chitin / Q000031:analogs & derivatives* / Q000378:metabolism; D048271:Chitosan; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction / Q000502:physiology; D004795:Enzyme Stability; D005285:Fermentation; D006026:Glycoside Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D013696:Temperature
D003470:Culture Media; D002686:Chitin; D048271:Chitosan; D006026:Glycoside Hydrolases; C019868:chitosanase