[Effects of zinc deficiency on the c-fos gene expression and transcription in the epiphyses of fetal mouse long bone in culture].
The effects of zinc on c-fos gene expression and transcription in the epiphyses of fetal long bone were studied in mice. The long bones of 16-day fetal mouse were cultured for 48 hours(in medium GBJb) and then used for measuring c-fos gene expression and transcription by immunohistochemistry and in situ hybridization. The result was analyzed by an imaged-analyses system. The experiment was divided into zinc control group(ZC), zinc deficiency group(ZD), zinc deficiency replenish group(ZDR) and zinc stimulatory group(ZS) respectively. The result showed that 1) zinc deficiency caused c-fos protein and mRNA expression and the number of reactive cell decreased. 2) when zinc concentration of medium was 100 mumol/L, the expression and transcription of c-fos gene were increased in hypertrophic, proliferative and resting zone of epiphyses. The research suggested that zinc could affect the expression of c-fos gene in mouse fetal long bone.
School of Public Health, West China University of Medical Science, Chengdu 610044, China.
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D000818:Animals; D002478:Cells, Cultured; D004838:Epiphyses / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005333:Fetus; D051379:Mice; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic; D014457:Ulna / Q000166:cytology / Q000378:metabolism; D015032:Zinc / Q000172:deficiency*
D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D015032:Zinc