[Homologous recombination and gene targeting].
Gene therapy and the production of mutated cell lines or model animals both require the development of efficient, controlled gene-targeting strategies. Classical approaches are based on the ability of cells to use homologous recombination to integrate exogenous DNA into their own genome. The low frequency of homologous recombination in mammalian cells leads to inefficient targeting. Here, we review the limiting steps of classical approaches and the new strategies developed to improve the efficiency of homologous recombination in gene-targeting experiments.
Biet
Elodie
E
UMR 2027 CNRS-Institut Curie, bâtiment 110, 15, rue Georges-Clémenceau, 91405 Orsay, France.
Larue
Lionel
L
Dutreix
Marie
M
D000818:Animals; D002460:Cell Line; D004249:DNA Damage; D004274:DNA, Recombinant / Q000235:genetics; D004277:DNA, Single-Stranded / Q000235:genetics; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D018390:Gene Targeting / Q000379:methods; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D008322:Mammals; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D011995:Recombination, Genetic; D012689:Sequence Homology, Nucleic Acid
D004274:DNA, Recombinant; D004277:DNA, Single-Stranded; D009838:Oligodeoxyribonucleotides