Enzymatic hydrolysis of ammonia-treated rice straw.
Rice straw pretreated with liquid anhydrous ammonia was hydrolyzed with cellulase, cellobiase, and hemicellulase. Ammonia-processing conditions were 1.5 g of NH3/g of dry matter, 85 degrees C, and several sample moisture contents. There were four ammonia addition time (min)-processing time (min) combinations. Sugars produced were analyzed as reducing sugars (dinitrosalicylic acid method) and by high-performance liquid chromatography. Monomeric sugars increased from 11% in the nontreated rice straw to 61% of theoretical in treated rice straw (79.2% conversion as reducing sugars). Production of monosaccharides was greater at higher moisture content and was processing time dependent. Glucose was the monosaccharide produced in greater amounts, 56.0%, followed by xylose, arabinose, and fructose, with 35.8, 6.6, and 1.4%, respectively.
Sulbarán-de-Ferrer
Betzabé
B
Lab. de Alimentos, Dept. de Química, Facultad de Ciencias, Universidad del Zulia, Av. Universidad, Grano de Oro. Módulo 2, Maracaibo, Venezuela.
Aristiguieta
Marielena
M
Dale
Bruce E
BE
Ferrer
Alexis
A
Ojeda-de-Rodriguez
Graciela
G
D000383:Agriculture / Q000295:instrumentation / Q000379:methods; D000641:Ammonia; D001089:Arabinose / Q000032:analysis; D019149:Bioreactors; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D004798:Enzymes / Q000378:metabolism; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006868:Hydrolysis; D007202:Indicators and Reagents; D009005:Monosaccharides / Q000032:analysis*; D012275:Oryza / Q000737:chemistry*; D011134:Polysaccharides / Q000302:isolation & purification; D014994:Xylose / Q000032:analysis
D004798:Enzymes; D007202:Indicators and Reagents; D009005:Monosaccharides; D011134:Polysaccharides; D000641:Ammonia; C007916:hemicellulose; D014994:Xylose; D001089:Arabinose; D005947:Glucose; D005690:Galactose