Anti-plasminogen autoantibodies from plasma of patients with systemic lupus erythematosus having anti-phospholipid antibody syndrome: isolation and some immunochemical properties.
Blood plasma samples from patients with systemic lupus erythematosus having the anti-phospholipid antibody syndrome were found to contain anti-plasminogen antibodies of the IgG class. The titers of anti-plasminogen autoantibodies of the IgG class were elevated in these patients compared with normal controls. Part of the pool of IgG anti-plasminogen antibodies reacts with an epitope in the lysine-binding sites of plasminogen. Anti-plasminogen IgG isolated from patients' blood plasma is specific only for a native epitope of human plasminogen passively adsorbed on immunosorbent micro-titration plate. As shown by enzyme immunoassay, autoantibodies to plasminogen of the IgG class cross-react with human fibrinogen.
Institute of Immunology, Russian Ministry of Health, Moscow, 115478 Russia.
L D
LD
Kozmin
I E
IE
Shirokova
T A
TA
Lisitsina
T V
TV
Popkova
T M
TM
Reschetnyak
A G
AG
Belenkiy
A I
AI
Martynov
O P
OP
Bliznukov
D000328:Adult; D015119:Aminocaproic Acid / Q000378:metabolism / Q000494:pharmacology; D016736:Antiphospholipid Syndrome / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology* / Q000378:metabolism; D001798:Blood Proteins / Q000276:immunology / Q000378:metabolism; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008875:Middle Aged; D010958:Plasminogen / Q000276:immunology*; D011485:Protein Binding
D001323:Autoantibodies; D001798:Blood Proteins; D007074:Immunoglobulin G; D010958:Plasminogen; D015119:Aminocaproic Acid