Evaluation of a new cellulose sponge-tipped swab for microbiological sampling: a laboratory and clinical investigation.
A new type of swab (Cellswab; Cellomeda, Turku, Finland), utilizing a highly absorbent cellulose viscose sponge material, was compared to some traditional swabs. The survival of 14 aerobic and 10 anaerobic and microaerophilic bacterial species in the Cellswab, two commercial swab transport systems (Copan, Brescia, Italy, and Orion Diagnostica, Espoo, Finland), and one Dacron swab (Technical Service Consultants Ltd. [TSC], Heywood, United Kingdom) was evaluated. Bacteria were suspended in broth, into which the swabs were dipped. The Cellswab absorbed 1.3 times more fluid and released 3.5 times more fluid upon plating than the other swabs. Aerobic bacteria were stored in dry tubes, the others in transport medium, at 4 degrees C and room temperature (RT), for up to 14 days. Swab samples were transferred to plates at 0, 1, 2, 4, 7, and 14 days. For 10 strains the Cellswab yielded > or =10% of the original CFU for longer than all the other swabs. In the clinical study, the ability of the Cellswab to detect beta-hemolytic streptococci from throat samples (n = 995) was compared to that of the TSC Dacron swab. The swabs performed equally, both when their samples were transferred to plates immediately and after storage for 1 day at 4 degrees C or RT. The changes in normal microbiota after storage were also similar. The Cellswab was found to perform at least as well as ordinary swabs. It was better at storing fastidious strains, and at keeping bacteria viable for long storage times; it might well be a useful replacement or complement to ordinary swabs.
National Public Health Institute, 20521 Turku, Finland. monica.osterblad@utu.fi
Monica
M
Osterblad
Helinä
H
Järvinen
Kurt
K
Lönnqvist
Solja
S
Huikko
Pekka
P
Laippala
Jouko
J
Viljanto
Heikki
H
Arvilommi
Pentti
P
Huovinen
D001424:Bacterial Infections / Q000175:diagnosis; D001431:Bacteriological Techniques / Q000295:instrumentation*; D002482:Cellulose; D015169:Colony Count, Microbial; D006069:Gonorrhea / Q000175:diagnosis; D006801:Humans; D009344:Neisseria gonorrhoeae / Q000302:isolation & purification; D010612:Pharyngitis / Q000175:diagnosis; D011093:Polyethylene Terephthalates; D013290:Streptococcal Infections / Q000175:diagnosis; D013291:Streptococcus / Q000302:isolation & purification; D013296:Streptococcus pneumoniae / Q000302:isolation & purification
D011093:Polyethylene Terephthalates; D002482:Cellulose