The "stomatin" gene and protein in overhydrated hereditary stomatocytosis.
In overhydrated hereditary stomatocytosis (OHSt), Coomassie- and silver-stained polyacrylamide gels show an apparently complete deficit of the 32-kDa membrane protein, stomatin. We have used an antistomatin antibody to examine peripheral blood films, bone marrow, splenic tissue, and hepatic tissue from these patients by immunocytochemistry. This technique revealed that, in fact, some red cells did show positive stomatin immunoreactivity; and consistent with this result, Western blot analysis of the red cell membranes confirmed that about one twentieth to one fiftieth of the normal amount of stomatin was in fact present. Flow cytometry, combining immunoreactive quantitation of stomatin expression with thiazole orange staining for reticulocytes, showed that in OHSt, it was the young cells that had more stomatin. Magnetic-activated cell separation studies, using beads to which an anti-transferrin receptor antibody was conjugated, confirmed that in OHSt there was a correspondence between expression of stomatin and the transferrin receptor. Immunocytochemistry and Western blotting revealed that in OHSt patients, the protein was present in spleen, liver, neutrophils, platelets, monocytes, and about 50% of the peripheral lymphocytes, with the same distribution as in healthy controls. Neither Southern blots, nor direct sequencing of multiple subclones of the cDNA, nor sequencing of amplicons from genomic DNA revealed any significant abnormality in stomatin gene sequence in these patients. The deficiency of stomatin from red cells appears to be due to a loss of stomatin from these red cells on maturation in the bone marrow and in the circulation.
Fricke
Britta
B
Departments of Medicine and Haematology, University College London, Rayne Institute, University Street, London WC1E 6JJ, United Kingdom.
Argent
Annette C
AC
Chetty
Margaret C
MC
Pizzey
Arnold R
AR
Turner
E Jane
EJ
Ho
Mei M
MM
Iolascon
Achille
A
von Düring
Monika
M
Stewart
Gordon W
GW
D000743:Anemia, Hemolytic / Q000235:genetics* / Q000378:metabolism*; D001798:Blood Proteins / Q000235:genetics* / Q000378:metabolism*; D015153:Blotting, Western; D001854:Bone Marrow Cells / Q000378:metabolism; D048429:Cell Size / Q000502:physiology; D004910:Erythrocyte Membrane / Q000378:metabolism; D004913:Erythrocytes, Abnormal / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression; D006801:Humans; D008297:Male; D021962:Membrane Microdomains / Q000378:metabolism; D008565:Membrane Proteins; D010375:Pedigree
D001798:Blood Proteins; D008565:Membrane Proteins; C036860:STOM protein, human