Screening of 25 compounds isolated from Phyllanthus species for anti-human hepatitis B virus in vitro.
Using an HBV-producing cell line and inhibition of the expression of the HBsAg and HBeAg as antiviral indicators, a study was conducted on 25 compounds isolated from four Phyllanthus (Euphorbiaceae) plants, including P. amarus Schum. & Thonn., P. multi florus Willd., P. tenellus Roxb. and P. virgatus Forst. f. It was found that niranthin (1), nirtetralin (3), hinokinin (5) and geraniin (13) at the non-cytotoxic concentration of 50 micro m, suppressed effectively both HBsAg and HBeAg expression, with the highest inhibition at 74.3%, 45.3%; 69.6%, 33.9%; 68.1%, 52.3%; 32.1%, 46.6%, respectively. Of these, niranthin (1) showed the best anti-HBsAg activity, while the most potent anti-HBeAg activity was observed with hinokinin (5).
National Research Institute of Chinese Medicine, No. 155-1, Sec. 2, Li Nung St., Peitou, Taipei, Taiwan, Republic of China.
Ray-Ling
RL
Huang
Yu-Ling
YL
Huang
Jun-Chih
JC
Ou
Chien-Chih
CC
Chen
Feng-Lin
FL
Hsu
Chungming
C
Chang
D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000494:pharmacology*; D000840:Anisoles / Q000302:isolation & purification / Q000494:pharmacology*; D052117:Benzodioxoles; D046508:Culture Techniques; D004149:Dioxoles / Q000302:isolation & purification / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis / Q000187:drug effects; D006513:Hepatitis B e Antigens / Q000096:biosynthesis / Q000187:drug effects; D006515:Hepatitis B virus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D006801:Humans; D047348:Hydrolyzable Tannins; D017705:Lignans; D015394:Molecular Structure; D029803:Phyllanthus; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D013634:Tannins / Q000302:isolation & purification / Q000494:pharmacology
D000840:Anisoles; D052117:Benzodioxoles; D004149:Dioxoles; D005960:Glucosides; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D047348:Hydrolyzable Tannins; D017705:Lignans; D010936:Plant Extracts; D013634:Tannins; C475934:hinokinin; C475932:niranthin; C475933:nirtetralin; C024603:Geraniin; D015107:4-Butyrolactone