Electroanalytical properties of aldehyde biosensors with a hybrid-membrane composed of an enzyme film and a redox Os-polymer film.
Aldehyde biosensors were constructed by cross-linking formaldehyde dehydrogenase (FDH) or aldehyde dehydrogenase (ADH) and bovine serum albumin on the surface of a redox Os-polymer-coated electrode. The prepared aldehyde biosensors responded rapidly (within 30 s) to aldehydes without the addition of a soluble mediator, because the inner redox Os-polymer film effectively mediated the electron transfer from NADH generated enzymatically into the outer enzyme film to a glassy carbon electrode. An FDH/Os-polymer electrode responded linearly over the concentration range of 2 x 10(-6)-5 x 10(-4) M for formaldehyde, while an ADH/Os-polymer electrode, though responding similarly to long chain aldehydes, such as propionaldehyde and butylaldehyde, responded linearly over the concentration range of 4 x 10(-6)-2 x 10(-4) M for acetaldehyde.
Yao
Toshio
T
Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, 1-1 Gakuencho, Sakai, Osaka 599-8531, Japan.
Handa
Shotaro
S
D000447:Aldehydes / Q000737:chemistry*; D015374:Biosensing Techniques; D008567:Membranes, Artificial; D010084:Oxidation-Reduction; D012680:Sensitivity and Specificity
D000447:Aldehydes; D008567:Membranes, Artificial