Purification, nanocrystallization and preliminary X-ray analysis of a C-terminal part of tropomodulin protein 1, isoform A, from Caenorhabditis elegans.
The C-terminal part of tropomodulin protein 1, isoform A, from Caenorhabditis elegans was expressed in Escherichia coli and purified to homogeneity. Optimized from the initial nanoscreen, crystals grew to dimensions of 0.25 x 0.15 x 0.15 mm at 277 K using 28.0%(v/v) PEG 400 as the precipitant by the hanging-drop vapor-diffusion technique. A data set of 94.9% completeness was collected to a resolution of 1.98 A at 100 K using a synchrotron X-ray source (SER-CAT). The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 31.7, b = 50.6, c = 107.1 A, and contained one molecule per asymmetric unit.
Ding
Haitao
H
Life Sciences College, Peking University, Beijing 100871, People's Republic of China.
Qiu
Shihong
S
Bunzel
Robert J
RJ
Luo
Danlin
D
Arabashi
Alireza
A
Lu
Shanyun
S
Symersky
Jindrich
J
Nagy
Lisa A
LA
DeLucas
Lawrence J
LJ
Li
Songlin
S
Luo
Ming
M
D000595:Amino Acid Sequence; D000818:Animals; D017173:Caenorhabditis elegans / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000378:metabolism; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D051348:Tropomodulin; D014961:X-Ray Diffraction
D029742:Caenorhabditis elegans Proteins; D011994:Recombinant Proteins; D051348:Tropomodulin; C480760:UNC-94 protein, C elegans