The generation and characterization of an ovary-selective cDNA library.
We have utilized the differential screening technique suppression subtractive hybridization to systematically isolate and clone genes that are expressed in an ovary-selective/specific manner. In total, 844 clones were sequenced and analyzed for homology to known genes using the basic local alignment tool. One hundred and fifty nine independent clones proved identical to previously-characterized genes whereas an additional 100 independent clones proved significantly homologous (but not identical) to previously-characterized genes. Yet 83 other independent clones did not display significant homology to previously-characterized genes now listed in the publicly-accessible non-redundant databases. As such, these latter genes were deemed novel. In this communication we focus on two such novel ovary-specific/hormonally-dependent genes, the full-length sequences of which were isolated using RACE technology. These ovary-selective genes may have significant implications for the understanding of ovarian function in molecular terms and for the development of innovative strategies for the promotion of fertility or its control.
Tanaka
Mamoru
M
Division of Reproductive Sciences, Department of Obstetrics and Gynecology, University of Utah Health Sciences Center, Salt Lake City, UT 84132, USA. mtanaka@sc.itc.keio.ac.jp
Hennebold
Jon D
JD
Miyakoshi
Kei
K
Teranishi
Takahide
T
Ueno
Kazunori
K
Adashi
Eli Y
EY
D000818:Animals; D018076:DNA, Complementary / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D015723:Gene Library; D051379:Mice; D009693:Nucleic Acid Hybridization; D009928:Organ Specificity; D010053:Ovary / Q000378:metabolism*
D018076:DNA, Complementary