title: Chiral analysis of amphetamine-type stimulants using reversed-polarity capillary electrophoresis/positive ion electrospray ionization tandem mass spectrometry.
abstract: Reversed-polarity (RP) capillary electrophoresis/positive ion electrospray ionization mass spectrometry (CE-ESI+ MS) and tandem mass spectrometry (MS/MS) were utilized for simultaneous chiral separation of nine amphetamine-type stimulants (ATS) (dl-norephedrine, dl-norpseudoephedrine, dl-ephedrine, dl-pseudoephedrine, dl-amphetamine, dl-methamphetamine, dl-methylenedioxyamphetamine, dl-methylenedioxymethamphetamine, and dl-methylenedioxyethylamphetamine). Using highly sulfated gamma-cyclodextrin (SU(XIII)-gamma-CD) as a chiral selector, the nine ATS were completely separated within 50 min. The migrated ATS-CD complex was dissociated at the ESI interface, and only ATS molecules went into the MS detector so that all 18 individual enantiomers were identified by their mass spectra. The detection limit of MS/MS was 10 times more sensitive than those for single MS. Seized d-methamphetamine hydrochloride samples dissolved at high concentration (20 mg/mL) were analyzed. Impurities originating in the precursor such as l-ephedrine and d-pseudoephedrine were detected and identified by tandem mass spectra.
authors.affiliation: National Research Institute of Police Science, Chiba, Japan. togawa@nrips.go.jp
authors.forename: Yuko T
authors.initials: YT
authors.lastname: Iwata
authors.forename: Tatsuyuki
authors.initials: T
authors.lastname: Kanamori
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Ohmae
authors.forename: Kenji
authors.initials: K
authors.lastname: Tsujikawa
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Inoue
authors.forename: Tohru
authors.initials: T
authors.lastname: Kishi
mesh_terms: D000662:Amphetamines / Q000032:analysis*; D000697:Central Nervous System Stimulants / Q000032:analysis*; D003505:Cyclodextrins; D019075:Electrophoresis, Capillary / Q000379:methods*; D016521:Electrophoresis, Gel, Pulsed-Field; D004867:Equipment Design; D005554:Forensic Medicine / Q000295:instrumentation / Q000379:methods; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013237:Stereoisomerism
chemical_list: D000662:Amphetamines; D000697:Central Nervous System Stimulants; D003505:Cyclodextrins