Automated RNA extraction by MagNA Pure followed by rapid quantification of cytokine and chemokine gene expression with use of fluorescence resonance energy transfer.
Loeffler
Juergen
J
Eberhard-Karls-Universität Tuebingen, Medizinische Klinik, 72076 Tuebingen, Germany. juergen.loeffler@med.uni-tuebingen.de
Swatoch
Phillipp
P
Akhawi-Araghi
Diana
D
Hebart
Holger
H
Einsele
Hermann
H
D001322:Autoanalysis; D018925:Chemokines / Q000032:analysis / Q000235:genetics; D016207:Cytokines / Q000032:analysis* / Q000235:genetics; D031541:Fluorescence Resonance Energy Transfer; D015870:Gene Expression; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry; D012313:RNA / Q000097:blood / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000032:analysis; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
D018925:Chemokines; D016207:Cytokines; D012333:RNA, Messenger; D011933:Reagent Kits, Diagnostic; D012313:RNA