title: Structures of immature flavivirus particles.
abstract: Structures of prM-containing dengue and yellow fever virus particles were determined to 16 and 25 A resolution, respectively, by cryoelectron microscopy and image reconstruction techniques. The closely similar structures show 60 icosahedrally organized trimeric spikes on the particle surface. Each spike consists of three prM:E heterodimers, where E is an envelope glycoprotein and prM is the precursor to the membrane protein M. The pre-peptide components of the prM proteins in each spike cover the fusion peptides at the distal ends of the E glycoproteins in a manner similar to the organization of the glycoproteins in the alphavirus spikes. Each heterodimer is associated with an E and a prM transmembrane density. These transmembrane densities represent either an EE or prMprM antiparallel coiled coil by which each protein spans the membrane twice, leaving the C-terminus of each protein on the exterior of the viral membrane, consistent with the predicted membrane-spanning domains of the unprocessed polyprotein.
authors.affiliation: Department of Biological Sciences, Lilly Hall, 915 West State Street, Purdue University, West Lafayette, IN 47907-2054, USA.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jeroen
authors.initials: J
authors.lastname: Corver
authors.forename: Paul R
authors.initials: PR
authors.lastname: Chipman
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Sergei V
authors.initials: SV
authors.lastname: Pletnev
authors.forename: Dagmar
authors.initials: D
authors.lastname: Sedlak
authors.forename: Timothy S
authors.initials: TS
authors.lastname: Baker
authors.forename: James H
authors.initials: JH
authors.lastname: Strauss
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Kuhn
authors.forename: Michael G
authors.initials: MG
authors.lastname: Rossmann
mesh_terms: D000818:Animals; D002460:Cell Line; D020285:Cryoelectron Microscopy; D003716:Dengue Virus / Q000737:chemistry / Q000235:genetics / Q000254:growth & development / Q000648:ultrastructure; D005416:Flavivirus / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development / Q000648:ultrastructure*; D007091:Image Processing, Computer-Assisted; D008051:Lipid Bilayers / Q000737:chemistry; D008958:Models, Molecular; D019251:Nucleocapsid / Q000737:chemistry / Q000648:ultrastructure; D012845:Sindbis Virus / Q000737:chemistry / Q000235:genetics / Q000254:growth & development / Q000648:ultrastructure; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics; D015005:Yellow fever virus / Q000737:chemistry / Q000235:genetics / Q000254:growth & development / Q000648:ultrastructure
chemical_list: D008051:Lipid Bilayers; D014764:Viral Proteins