An archaeal homing endonuclease I-PogI cleaves at the insertion site of the neighboring intron, which has no nested open reading frame.
Homing endonucleases (HEs) of the LAGLIDADG family cleave intron/inteinless cognate DNA at, or near, the insertion site (IS) of their own intron/intein. Here, we describe a notable exception to this rule. Two introns, Pog.S1205 (length 32 bp) and Pog.S1213 (664 bp), whose ISs are 8 bp apart, exist within the 16S rRNA gene of the archaeon Pyrobaculum oguniense. Pog.S1213 harbors a nested open reading frame (ORF) encoding a 22 kDa monomeric protein, I-PogI, which contains two LAGLIDADG motifs and has optimal DNA cleavage activity at 90 degrees C. Intriguingly, I-PogI cleaves the Pog.S1205-less substrate DNA in the presence or absence of Pog.S1213. The cleavage site (CS) of I-PogI does not coincide with the IS of Pog.S1213 but with that of Pog.S1205. Thus, I-PogI activity both promotes the homing of its own intron, Pog.S1213, and guarantees co-conversion of the ORF-less intron Pog.S1205.
Nakayama
Hitoshi
H
Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Japan.
Morinaga
Yayoi
Y
Nomura
Norimichi
N
Nunoura
Takuro
T
Sako
Yoshihiko
Y
Uchida
Aritsune
A
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001105:Archaea / Q000235:genetics*; D001483:Base Sequence; D002384:Catalysis; D004275:DNA, Ribosomal / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004720:Endonucleases / Q000494:pharmacology*; D005091:Exons; D007438:Introns; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D012336:RNA, Ribosomal, 16S / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013696:Temperature
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D004720:Endonucleases; C475141:endonuclease I-PogI