Mutation or drug-dependent microtubule disruption causes radial swelling without altering parallel cellulose microfibril deposition in Arabidopsis root cells.
As critical determinants of growth anisotropy in plants, cortical microtubules are thought to constrain the movement of cellulose synthase complexes and thus align newly deposited cellulose microfibrils. We tested this cellulose synthase constraint model using the temperature-sensitive mor1-1 mutant of Arabidopsis. Contrary to predictions, the disruption of cortical microtubules in mor1-1 root epidermal cells led to left-handed root twisting and radial swelling but did not alter the transverse orientation of cellulose microfibrils. We also found that drug-dependent disassembly or hyperstabilization of cortical microtubules did not alter the parallel order of cellulose microfibrils. By measuring cellulose content in mor1-1 seedlings, we verified that cellulose synthesis is not reduced at the restrictive temperature. The independence of cortical microtubule organization and cellulose microfibril alignment was supported by the observation that double mutants of mor1-1 and rsw1-1, the cellulose-deficient mutant with misaligned microfibrils, had additive phenotypes. Our results suggest that cortical microtubules regulate growth anisotropy by some mechanism other than cellulose microfibril alignment or synthesis.
Sugimoto
Keiko
K
Plant Cell Biology Group, Research School of Biological Sciences, The Australian National University, Canberra ACT 2601, Australia.
Himmelspach
Regina
R
Williamson
Richard E
RE
Wasteneys
Geoffrey O
GO
D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002482:Cellulose / Q000378:metabolism*; D004136:Dinitrobenzenes / Q000494:pharmacology; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism*; D009154:Mutation; D017239:Paclitaxel / Q000494:pharmacology; D010641:Phenotype; D019441:Plant Epidermis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D018517:Plant Roots / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D013424:Sulfanilamides; D013696:Temperature
D004136:Dinitrobenzenes; D013424:Sulfanilamides; C012465:oryzalin; D002482:Cellulose; D017239:Paclitaxel