[No switch region is found in the regulatory sequence of HPV 16 for promoter P97].
OBJECTIVE
Cellular transcriptional regulatory factor YY1 represses the activity of the early promoter P97 of HPV 16, but the regulatory activity to early promoter P105 of HPV 18 depends on a switch region which locates at the upstream sequences of YY1 binding sites. This study is to find out whether within the HPV 16 LCR there is such motif.


METHODS
DNA sequences of the whole HPV 16 LCR region were analyzed. Two similar switch structures were found. Different CAT reporter plasmids, one containing HPV 16 reference and the other mutated LCRs, both starting at the end of L1 and from the beginning of the enhancer, were generated and transfected in HeLa cells transiently.


RESULTS
CAT assays showed there is no change of regulation pattern of YY1 on P97, when the two switch-similar structures were deleted. Sequences analysis also showed that no switch region like motif could be demonstrated in enhancer region, in promoter proximal segment, and even in E6/E7 area.


CONCLUSIONS
It suggests that there is no such switch region within HPV 16 transcriptional regulatory region.
Dong
X
X
Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052.
Liu
H
H
Pfister
H
H
D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006367:HeLa Cells; D006801:Humans; D027383:Papillomaviridae / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000378:metabolism
D004279:DNA, Viral; D014157:Transcription Factors