[Dual cell seeding to improve cell retention on polytetrafluoroethylene grafts].
OBJECTIVE
To improve cell retention on the graft with dual cell seeding technique, adding a layer of smooth muscle cells (SMCs) between the graft and endothelial cells (ECs).


METHODS
Polytetrafluoroethylene (PTFE) grafts pretreated with fibronectin were seeded with SMC followed by ECs one day later and exposure to an in vitro flow system. The number of ECs on grafts was counted under microscope.


RESULTS
After exposure to the flow for 1 hour, 61% of the ECs lost when the ECs were seeded alone, whereas only 27% of the ECs lost when the ECs were seeded on the top of SMCs. Preconditioning of a cell seeded graft in a low rate flow did not improve cell retention when late exposure to a higher rate flow.


CONCLUSIONS
This in vitro study indicates that using SMCs as a media layer between ECs and graft surface can improve the retention of seeded ECs on PTFE graft.
Chen
Li
L
Department of Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China.
Yu
Hong
H
Dai
Ning
N
Tao
Si-feng
SF
Gong
Wei-hua
WH
D001807:Blood Vessel Prosthesis; D002448:Cell Adhesion; D042783:Endothelial Cells / Q000166:cytology; D005353:Fibronectins / Q000494:pharmacology; D006801:Humans; D032389:Myocytes, Smooth Muscle / Q000166:cytology; D011138:Polytetrafluoroethylene; D011474:Prosthesis Design; D023822:Tissue Engineering / Q000379:methods*
D005353:Fibronectins; D011138:Polytetrafluoroethylene