title: Differentiation of Borrelia burgdorferi sensu lato through groEL gene analysis.
abstract: The nucleotide sequences (310 bp) of the groEL gene, which encode the 60-kDa heat shock protein GroEL from 31 reference strains of Borrelia were determined and compared. More than 92.3% similarity was observed among Borrelia burgdorferi sensu lato strains. In the phylogenetic tree constructed with the maximum-likelihood method, each species of B. burgdorferi sensu lato was differentiated as a distinct entity. We developed polymerase chain reaction-restriction fragment length polymorphism analysis using a specific single amino acid variation [N(213) (AAT)-->S (AGC or AGT)] between B. burgdorferi sensu stricto strains and the other B. burgdorferi sensu lato strains. These results showed that the groEL gene is useful for differentiation of B. burgdorferi sensu lato.
authors.affiliation: Department of Microbiology, College of Medicine, Konkuk University, 322 Danwol-dong, Chugju, Chungchungbuk-Do 380-701, South Korea.
authors.forename: Seung-Hyun
authors.initials: SH
authors.lastname: Lee
authors.forename: Jung-Hee
authors.initials: JH
authors.lastname: Lee
authors.forename: Hyo-Soon
authors.initials: HS
authors.lastname: Park
authors.forename: Won-Jong
authors.initials: WJ
authors.lastname: Jang
authors.forename: Seong-Eun
authors.initials: SE
authors.lastname: Koh
authors.forename: Young-Mok
authors.initials: YM
authors.lastname: Yang
authors.forename: Bum-Joon
authors.initials: BJ
authors.lastname: Kim
authors.forename: Yoon-Hoh
authors.initials: YH
authors.lastname: Kook
authors.forename: Kyung-Hee
authors.initials: KH
authors.lastname: Park
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D025065:Borrelia burgdorferi / Q000145:classification* / Q000235:genetics* / Q000254:growth & development; D018834:Chaperonin 60 / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA
chemical_list: D018834:Chaperonin 60; D004269:DNA, Bacterial