title: The TRPV6 gene, cDNA and protein.
abstract: The mouse TRPV6 gene is localized on chromosome 6 and extends over 15.66kb. The encoded protein comprises 727 amino acid residues with a calculated relative molecular mass of 83,210Da. TRPV6 is glycosylated and both variants, the glycosylated and the de-glycosylated proteins, are recognized by various polyclonal and monoclonal antibodies, which were raised against TRPV6. Like human TRPV6, mouse TRPV6 binds calmodulin in the presence, but not in the absence of Ca2+. TRPV6 is abundantly expressed in mouse pancreas and placenta, and to a much lesser extend in mouse stomach and kidney. No transcript expression was detected in poly(A)+RNA isolated from heart, brain, intestine, esophagus or aortic endothelial cells.
authors.affiliation: Experimentelle und Klinische Pharmakologie und Toxikologie, Universität des Saarlandes, D 66421 Homburg, Germany.
authors.forename: Daniela
authors.initials: D
authors.lastname: Hirnet
authors.forename: Jenny
authors.initials: J
authors.lastname: Olausson
authors.forename: Claudia
authors.initials: C
authors.lastname: Fecher-Trost
authors.forename: Matthias
authors.initials: M
authors.lastname: Bödding
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Nastainczyk
authors.forename: Ulrich
authors.initials: U
authors.lastname: Wissenbach
authors.forename: Veit
authors.initials: V
authors.lastname: Flockerzi
authors.forename: Marc
authors.initials: M
authors.lastname: Freichel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D002147:Calmodulin / Q000378:metabolism; D002478:Cells, Cultured; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary / Q000737:chemistry* / Q000235:genetics; D006031:Glycosylation; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology; D010920:Placenta / Q000737:chemistry*; D011061:Poly A / Q000235:genetics; D011817:Rabbits; D017386:Sequence Homology, Amino Acid; D050916:TRPV Cation Channels; D014018:Tissue Distribution
chemical_list: D015220:Calcium Channels; D002147:Calmodulin; D018076:DNA, Complementary; D010446:Peptide Fragments; D050916:TRPV Cation Channels; C120776:TRPV6 channel; D011061:Poly A; D002118:Calcium