[The in vivo formation of cementum-like tissue by bovine cementoblasts].
OBJECTIVE
To test the bovine cementoblasts (CBs) cementum-forming ability in vivo.


METHODS
Root fragments of newborn bovine freshly extracted mandibular incisor were cultured routinely and 4th-5th passages of CBs were harvested. CBs were then cultured in the medium supplemented with 50 mg/L alpha-ascorbic acid and 10 mmol/l beta-glycerolphosphate to form a thick layer as tissue engineering scaffold for cementum formation. Collagen membrane was used as control scaffold. 2 x 10(6) cells were attached to the CBs-made carrier as well as collagen membrane scaffolds and transplanted subcutaneously into immunodeficient mice. Transplants were harvested at 7th week. Histological sections were stained with HE, alizarin red S and van Kossa methods as well as monoclonal Ab against bovine cementum attachment protein (CAP).


RESULTS
CBs-made scaffold supported more cementum-like tissue (CLT) formation than collagen-made scaffold. The CLT formed on CBs scaffold was partly calcified with embedded cells. Uncalcified cementoid-like material could be seen on the surface and was encircled by cubical CB-like cells. The CLT was also positive to CAP and van Kossa staining.


CONCLUSIONS
These results suggest that the bovine CBs can form cementum-like tissue. The cell-made carrier is a better scaffold than collagen membrane.
Hou
Jian-xia
JX
Department of Periodontology, Peking University School of Stomatology, Beijing 100081, China.
Cao
Cai-fang
CF
Meng
Huan-xin
HX
D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D016025:Bone Transplantation / Q000379:methods*; D002417:Cattle; D015815:Cell Adhesion Molecules / Q000032:analysis; D002478:Cells, Cultured; D003739:Dental Cementum / Q000737:chemistry / Q000166:cytology / Q000637:transplantation*; D007150:Immunohistochemistry; D058574:Integrin-Binding Sialoprotein; D008297:Male; D051379:Mice; D008819:Mice, Nude; D015675:Osteocalcin / Q000032:analysis; D015676:Osteonectin / Q000032:analysis; D012795:Sialoglycoproteins / Q000032:analysis; D023822:Tissue Engineering / Q000379:methods*; D014183:Transplantation, Heterologous
D015815:Cell Adhesion Molecules; C546872:Ibsp protein, mouse; D058574:Integrin-Binding Sialoprotein; D015676:Osteonectin; D012795:Sialoglycoproteins; C433705:cementum attachment protein, 55-kDa; D015675:Osteocalcin; D000469:Alkaline Phosphatase