title: Notch activity is required to maintain floorplate identity and to control neurogenesis in the chick hindbrain and spinal cord.
abstract: Notch signalling plays a major role in many invertebrate and vertebrate patterning systems. In this paper, we use high-titre, non-replicative pseudotype viruses to show that the two Notch ligands, Delta1 and Serrate1 (Jagged1), have differing activities in the developing chick spinal cord and hindbrain. In the walls of the neural tube, Serrate1 appears not to affect neurogenesis, in contrast to Delta1 which mediates lateral inhibition as elsewhere in the nervous system. In the floorplate we find that there is also a requirement for Notch, but with a different type of dependence on the two Notch ligands: cells with a floorplate character are lost when Notch activity is blocked with dominant-negative, truncated forms of either Delta1 or Serrate1. Our results are consistent with ligand-receptor specificity within the Notch signalling pathway, Serrate1 recognising selectively Notch2 (which is expressed in the floorplate), and Delta1 acting on both Notch2 and Notch1 (which is expressed in the walls of the neural tube).
authors.affiliation: London Research Institute, Cancer Research UK, London, UK.
authors.forename: Isabelle
authors.initials: I
authors.lastname: le Roux
authors.forename: Julian
authors.initials: J
authors.lastname: Lewis
authors.forename: David
authors.initials: D
authors.lastname: Ish-Horowicz
mesh_terms: D000818:Animals; D001483:Base Sequence; D002135:Calcium-Binding Proteins; D002642:Chick Embryo; D004247:DNA / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D036341:Intercellular Signaling Peptides and Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008024:Ligands; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D011506:Proteins / Q000235:genetics / Q000502:physiology; D051881:Receptor, Notch1; D051883:Receptor, Notch2; D011956:Receptors, Cell Surface / Q000502:physiology; D051880:Receptors, Notch; D012190:Retroviridae / Q000235:genetics; D012249:Rhombencephalon / Q000196:embryology*; D000072099:Serrate-Jagged Proteins; D015398:Signal Transduction; D013116:Spinal Cord / Q000196:embryology*; D014157:Transcription Factors; D014162:Transfection
chemical_list: D002135:Calcium-Binding Proteins; D036341:Intercellular Signaling Peptides and Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008024:Ligands; D008565:Membrane Proteins; D011506:Proteins; D051881:Receptor, Notch1; D051883:Receptor, Notch2; D011956:Receptors, Cell Surface; D051880:Receptors, Notch; D000072099:Serrate-Jagged Proteins; D014157:Transcription Factors; C064045:delta protein; D004247:DNA