A murine model of cholesteatoma-induced bone resorption using autologous dermal implantation.
OBJECTIVE
To investigate a novel murine model for dermal implant-induced osteolysis analogous to bone resorption observed in middle ear cholesteatoma.


METHODS
Animal experiment.


METHODS
We placed autologous dermal implants on the surface of mouse calvaria. The calvaria were examined at days 1, 3, 5, 7, and 14 after implantation by histological study and tartrate-resistant acid phosphatase immunohistochemical processing to detect osteoclasts.


RESULTS
Dermal implants showed a significantly increased osteoclast density compared with control samples. The dermal implant tissue remained viable and produced a robust, localized inflammatory osteolytic response on the adjacent calvarial surface. Osteoclasts were predominantly found on the surface of the calvarium with the greatest osteoclast density seen at 5 to 7 days after implantation.


CONCLUSIONS
The mouse model is expected to be a useful tool for investigating the pathogenesis of localized inflammatory bone resorption related to cholesteatoma.
Sudhoff
Holger
H
Department of Otorhinolaryngology-Head and Neck Surgery, University of Bochum, Germany. Holger.Sudhoff@ruhr-uni-bochum.de
Liebehenz
Yvonne
Y
Aschenbrenner
Jessica
J
Jung
Jae
J
Hildmann
Henning
H
Dazert
Stefan
S
D000818:Animals; D001862:Bone Resorption / Q000473:pathology*; D018424:Cholesteatoma, Middle Ear / Q000473:pathology*; D020405:Dermis / Q000637:transplantation; D004195:Disease Models, Animal; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D010010:Osteoclasts / Q000473:pathology; D010014:Osteolysis / Q000473:pathology; D012886:Skull / Q000473:pathology