title: Localization of GPI-80, a beta2-integrin-associated glycosylphosphatidyl-inositol anchored protein, on strongly CD14-positive human monocytes.
abstract: Human monocyte/macrophage systems are extremely heterogeneous. Although many attempts have been made to define monocyte subpopulations, few antigens distinguish them. We previously reported that GPI-80, a novel glycosylphosphatidyl-inositol (GPI)-anchored protein that is expressed mainly on human neutrophils regulates neutrophil adherence and migration, and that GPI-80 is expressed on monocytes. In this study, we examined the precise distribution of GPI-80-positive monocytes using flow cytometry. Using anti-CD14 and anti-CD16 mAbs, almost all GPI-80-bearing monocytes belong to the strongly CD14-positive monocyte subpopulation. Furthermore, flow cytometric analysis of GPI-80 and other monocyte markers revealed that GPI-80 expression was high in CD11b-, CD32-, and CD64-positive monocytes. In contrast, GPI-80 expression was low in HLA-DQ-positive monocytes. These results suggest that almost all GPI-80 positive monocytes belong to a monocyte subpopulation that is superior in phagocytosis and reactive oxygen production, but inferior in antigen presentation. GPI-80 may be a useful antigen for classifying monocytes into subpopulations.
authors.affiliation: Department of Immunology & Parasitology, Yamagata University School of Medicine, 2-2-2 Iida-Nishi, Yamagata 990-9585, Japan.
authors.forename: Dai
authors.initials: D
authors.lastname: Sendo
authors.forename: Yuji
authors.initials: Y
authors.lastname: Takeda
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ishikawa
authors.forename: Fujiro
authors.initials: F
authors.lastname: Sendo
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Araki
mesh_terms: D000581:Amidohydrolases; D000911:Antibodies, Monoclonal / Q000378:metabolism; D018821:CD18 Antigens / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D005434:Flow Cytometry; D058851:GPI-Linked Proteins; D017261:Glycosylphosphatidylinositols / Q000737:chemistry*; D006801:Humans; D006867:Hydrolases; D018950:Lipopolysaccharide Receptors / Q000096:biosynthesis*; D009000:Monocytes / Q000276:immunology / Q000378:metabolism*; D009504:Neutrophils / Q000378:metabolism; D010587:Phagocytosis; D017382:Reactive Oxygen Species; D017452:Receptors, IgG / Q000096:biosynthesis
chemical_list: D000911:Antibodies, Monoclonal; D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; D058851:GPI-Linked Proteins; D017261:Glycosylphosphatidylinositols; D018950:Lipopolysaccharide Receptors; D017382:Reactive Oxygen Species; D017452:Receptors, IgG; D006867:Hydrolases; D000581:Amidohydrolases; C121771:VNN2 protein, human