[Expression of P-selectin in nasal polyps and its mechanisms].
OBJECTIVE
To investigate the expression of P-selectin and its mechanism in nasal polyps.


METHODS
The expression of P-selectin was detected with immunohistochemistry and reverse transcriptase polymerase chain reaction (RT-PCR) in nasal polyps from 24 patients and nasal epithelium from 9 normals. The production of P-selectin in supernatans of isolated cultured vascular endothelial cells induced by interleukin-5 (IL-5) was detected with enzyme-linked immunosorbent assay (ELISA).


RESULTS
The positive rate of P-selectin in nasal polyps was 79.1% (19/24) while it was 22.2%(2/9) in normals. The relative density ratio of P-selectin and beta-actin were 0.68 +/- 0.11 in nasal polyps and 0.29 +/- 0.08 in normals indicated by RT-PCR and agrose electrophoresis. The concentration of P-selectin was (53.4 +/- 2.9) ng/L and (21.3 +/- 3.5) ng/L in the two groups respectively.


CONCLUSIONS
P-selectin upregulated in nasal polyps correlated with the infiltrated eosinophils and IL-5 could induce the expression of P-selectin protein in the isolated cultured endothelial cells. It suggested that upregulation of P-selectin may play a role in the pathogenesis of nasal polyps and can be enhanced by cytokine IL-5.
Li
Huabin
H
Department of Otorhinolaryngology Head and Neck Surgery, Third Affiliated Hospital, Sun Yet-sen University, Guangzhou 510630, China. lhb7351@hotmail.com
Xu
Geng
G
Li
Yuan
Y
Xie
Minqiang
M
Xu
Rui
R
D000293:Adolescent; D000328:Adult; D004730:Endothelium, Vascular / Q000378:metabolism; D005260:Female; D006801:Humans; D015848:Interleukin-5 / Q000032:analysis; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000378:metabolism*; D019007:P-Selectin / Q000096:biosynthesis*
D015848:Interleukin-5; D019007:P-Selectin