[Structural and functional changing induced by exposure to adaptive doses of X-rays in the human lymphocytes both normal and defective by reparation of DNA double strands breaks].
In the present work it is shown that the phenomenon of interphase chromosome centromeric region displacement, earlier revealed by the authors, is not realized in G0-lymphocytes with heterozygous BRCA1/2 gene mutations. The role of these genes in DNA double strand break (DSB) reparation is known. It is concluded that chromosome locus displacement is necessary for DSB repair, at least in the process of homologous recombination. In accordance with our data, some feature (pericentromeric cluster disintegration and displacement, the nucleus size increasing) characteristic for S- and G0-lymphocytes are observed in normal G0-lymphocytes treated with 3 and 10 cGy. However, the size of nucleus in G0-lymphocytes is restored through 6 hours after irradiation in opposite to the process in dividing cells. It was proposed that some typical for resting cell functions of G0-lymphocytes after inducing by adaptive doze of radiation are stopped as similarly as after stimulation of cells. Interestingly, that the process of the induced chromosome loci displacement is correlated with the decreasing of DNA reparation possibilities under UV-irradiation. The induced apoptosis level also decreases when chromosome loci are displaced. The possible mechanisms of the revealed phenomenon are discussed. This research supported by RFBR grant (No. 01-04-49180).
Spitkovskiĭ
D M
DM
State Research Center for Medical Genetics, Russian Academy of Medical Sciences, Moscow, 115478 Russia.
Veĭko
N N
NN
Ermakov
A V
AV
Kuz'mina
I V
IV
Makarenkov
A S
AS
Salimov
A G
AG
Terekhov
S M
SM
Karpukhin
A V
AV
D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D002467:Cell Nucleus / Q000528:radiation effects; D002503:Centromere / Q000528:radiation effects; D004247:DNA / Q000528:radiation effects; D004249:DNA Damage / Q000528:radiation effects; D004260:DNA Repair / Q000528:radiation effects; D019398:Genes, BRCA1 / Q000528:radiation effects*; D024522:Genes, BRCA2 / Q000528:radiation effects*; D007399:Interphase / Q000528:radiation effects; D008214:Lymphocytes / Q000166:cytology / Q000528:radiation effects*; D051379:Mice; D005810:Multigene Family; D009154:Mutation; D011829:Radiation Dosage; D013997:Time Factors; D014466:Ultraviolet Rays
D004247:DNA