Kinetic studies of attachment and detachment of microbial cells from soil.
A mathematical model was developed to describe the kinetics of cell attachment and detachment from soil. Soil-column experiments were performed to evaluate the model parameters. Pseudomonas putida G7 capable of degrading naphthalene was used as a model microorganism. A sediment sample taken from an uncontaminated area near a coal tar waste site in upstate New York, USA was used as a test soil. The kinetics of cell attachment and detachment from the model soil could be described by the developed first-order model. The equilibrium constant of attachment (11.4 ml g(-1)), the rate coefficient of cell attachment (0.299 ml g(-1) min(-1) and the rate coefficient of cell detachment (0.0263 min(-1)) were determined from the soil-column experiment. The equilibrium constant of attachment determined in this study (11.4 ml g(-1)) was within the range of those reported in the literature for bacterial attachment to soil (0.55 to 12.6 ml g(-1)). The kinetic model succesfully predicted the data of batch experiment for cell attachment and detachment soil.
Ahn
I S
IS
Department of Chemical Engineering, Yonsei University Seoul, 120-749, Korea.
Lee
C H
CH
D000327:Adsorption; D001673:Biodegradation, Environmental; D007700:Kinetics; D008962:Models, Theoretical; D009281:Naphthalenes / Q000378:metabolism; D016958:Pseudomonas putida / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
D009281:Naphthalenes; D012989:Soil Pollutants; C031721:naphthalene