title: Comparison of proteins synthesized by polarized caprine oviductal epithelial cells and oviductal explants in vitro.
abstract: Our objectives were to compare proteins secreted by caprine oviductal explants and oviductal epithelial (OE) cells in vitro. Oviducts were collected from goats on Days 1 (n=5) and 5 (n=5) of the estrous cycle. Radiolabeled secretory proteins from tissue segments and cell cultures were visualized using SDS-PAGE and fluorography. After culture, media from ampulla oviduct segments collected on Days 1 and 5 of the estrous cycle contained an acidic 97 kDa protein, which was greatly reduced in culture medium obtained from infundibulum and isthmus oviduct segments. A complex of low molecular weight proteins (14-26 kDa) could be modulated by estradiol when OE cells were cultured on plastic. This complex was constitutively expressed when OE cells were cultured on Matrigel-coated filters. Polarized OE cells were also capable of compartment-specific secretion of [L-(35)S]-methionine-labeled proteins. A 45 kDa acidic protein was predominantly secreted into the apical compartment while a 66 kDa acidic protein was preferentially localized in the basal compartment. Proteins secreted by OE cells were similar to proteins secreted by tissue segments in vitro. Therefore, under well-defined culture conditions OE cells may be useful in enhancing in vitro fertilization or early embryonic development.
authors.affiliation: Cooperative Agricultural Research Center, PO Box 4079, Prairie View A and M University, Prairie View, TX 77446-4079, USA.
authors.forename: S
authors.initials: S
authors.lastname: Woldesenbet
authors.forename: G R
authors.initials: GR
authors.lastname: Newton
mesh_terms: D000818:Animals; D002455:Cell Division; D016764:Cell Polarity; D002478:Cells, Cultured; D046508:Culture Techniques; D015180:Electrophoresis, Gel, Two-Dimensional; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D030762:Estrous Cycle; D005187:Fallopian Tubes / Q000166:cytology*; D005260:Female; D006041:Goats / Q000378:metabolism*; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism; D013462:Sulfur Radioisotopes
chemical_list: D011506:Proteins; D013462:Sulfur Radioisotopes