title: [Expression of P-selectin in nasal polyps and its mechanisms].
abstract: OBJECTIVE
  To investigate the expression of P-selectin and its mechanism in nasal polyps.
  
  
  METHODS
  The expression of P-selectin was detected with immunohistochemistry and reverse transcriptase polymerase chain reaction (RT-PCR) in nasal polyps from 24 patients and nasal epithelium from 9 normals. The production of P-selectin in supernatans of isolated cultured vascular endothelial cells induced by interleukin-5 (IL-5) was detected with enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  The positive rate of P-selectin in nasal polyps was 79.1% (19/24) while it was 22.2%(2/9) in normals. The relative density ratio of P-selectin and beta-actin were 0.68 +/- 0.11 in nasal polyps and 0.29 +/- 0.08 in normals indicated by RT-PCR and agrose electrophoresis. The concentration of P-selectin was (53.4 +/- 2.9) ng/L and (21.3 +/- 3.5) ng/L in the two groups respectively.
  
  
  CONCLUSIONS
  P-selectin upregulated in nasal polyps correlated with the infiltrated eosinophils and IL-5 could induce the expression of P-selectin protein in the isolated cultured endothelial cells. It suggested that upregulation of P-selectin may play a role in the pathogenesis of nasal polyps and can be enhanced by cytokine IL-5.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Third Affiliated Hospital, Sun Yet-sen University, Guangzhou 510630, China. lhb7351@hotmail.com
authors.forename: Huabin
authors.initials: H
authors.lastname: Li
authors.forename: Geng
authors.initials: G
authors.lastname: Xu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.forename: Minqiang
authors.initials: M
authors.lastname: Xie
authors.forename: Rui
authors.initials: R
authors.lastname: Xu
mesh_terms: D000293:Adolescent; D000328:Adult; D004730:Endothelium, Vascular / Q000378:metabolism; D005260:Female; D006801:Humans; D015848:Interleukin-5 / Q000032:analysis; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000378:metabolism*; D019007:P-Selectin / Q000096:biosynthesis*
chemical_list: D015848:Interleukin-5; D019007:P-Selectin