title: 5-Lipoxygenase knockout mice exhibit a resistance to pleurisy and lung injury caused by carrageenan.
abstract: In the present study, by comparing the responses in wild-type (WT) mice and mice lacking [knockout (KO)] the 5-lipoxygenase (5-LO), we investigated the role played by 5-LO in the development of acute inflammation. When compared with carragenan-treated 5-LOWT mice, 5-LOKO mice, which had received carrageenan, exhibited a reduced degree of pleural exudation, polymorphonuclear cell migration. Lung myeloperoxidase activity, an index of neutrophil infiltration, was significantly reduced in 5-LOKO mice in comparison with 5-LOWT. Lung-tissue sections from carrageenan-treated 5-LOWT mice showed positive staining for intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), P-selectin, and E-selectin, which were mainly localized around vessels. The intensity and degree of ICAM-1, VCAM-1, P-selectin, and E-selectin were markedly reduced in tissue section from carrageenan-5-LOKO mice, which also improved the histological status of the inflamed lungs. Taken together, our results clearly demonstrate that 5-LO modulates neutrophil infiltration in the acute lung inflammation.
authors.affiliation: Department of Clinical and Experimental Medicine, University Federico II, Napoli, Italy. salvator@unime.it
authors.forename: Salvatore
authors.initials: S
authors.lastname: Cuzzocrea
authors.forename: Antonietta
authors.initials: A
authors.lastname: Rossi
authors.forename: Ivana
authors.initials: I
authors.lastname: Serraino
authors.forename: Emanuela
authors.initials: E
authors.lastname: Mazzon
authors.forename: Rosanna
authors.initials: R
authors.lastname: Di Paola
authors.forename: Laura
authors.initials: L
authors.lastname: Dugo
authors.forename: Tiziana
authors.initials: T
authors.lastname: Genovese
authors.forename: Barbara
authors.initials: B
authors.lastname: Calabrò
authors.forename: Achille P
authors.initials: AP
authors.lastname: Caputi
authors.forename: Lidia
authors.initials: L
authors.lastname: Sautebin
mesh_terms: D000818:Animals; D001094:Arachidonate 5-Lipoxygenase / Q000235:genetics / Q000502:physiology*; D002351:Carrageenan; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D007150:Immunohistochemistry; D051379:Mice; D018345:Mice, Knockout; D020556:Neutrophil Infiltration; D009569:Nitric Oxide / Q000096:biosynthesis; D010998:Pleurisy / Q000139:chemically induced / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D011014:Pneumonia / Q000139:chemically induced / Q000209:etiology* / Q000378:metabolism / Q000473:pathology
chemical_list: D015815:Cell Adhesion Molecules; D009569:Nitric Oxide; D002351:Carrageenan; D001094:Arachidonate 5-Lipoxygenase