[Role of expression inducible nitric oxide synthase-mRNA of leukocyte and nasal mucosa in allergic rhinitis].
OBJECTIVE
To investigate the relationship between expression of leukocyte inducible nitric oxide synthase (iNOS)-mRNA and allergic rhinitis (AR).


METHODS
Thirty-five patients with AR and 30 healthy controls were included in this study. Expression of iNOS-mRNA in peripheral blood leukocyte was detected by in situ hybridization. NO in plasm was measured by nitrate reductase. Expression of iNOS-mRNA in nasal mucosal was detected in 8 patients with AR and 6 healthy controls.


RESULTS
No expression of leukocyte iNOS-mRNA in healthy controls was found. In AR patients, the positive cells were significantly increased, the positive rate reached 40.82%. Expression of iNOS-mRNA was localized at the epithelium, gland and macrophage in healthy controls. Hyperplasia and expression of iNOS-mRNA increased at epithelium, gland and macrophage in the AR patients(t = 23.17, P < 0.001). The level of plasm NO in AR group was higher than that in healthy control group (t = 27.89, P < 0.001).


CONCLUSIONS
There is a relationship between expression of leukocyte iNOS-mRNA and the level of plasm NO in AR patients. The study provides an easy method of in situ hybridization for detecting some signal in body.
Zhang
Hui
H
Department of Otorhinolaryngology, Second Hospital of Shandong University, Jinan 250033, China.
Zhang
Da-liang
DL
Li
Rui-feng
RF
Guo
Cheng-hao
CH
D000328:Adult; D005260:Female; D006801:Humans; D007962:Leukocytes / Q000201:enzymology*; D008297:Male; D009297:Nasal Mucosa / Q000201:enzymology*; D009569:Nitric Oxide / Q000097:blood; D019001:Nitric Oxide Synthase / Q000096:biosynthesis* / Q000235:genetics; D052247:Nitric Oxide Synthase Type II; D012333:RNA, Messenger / Q000096:biosynthesis; D012221:Rhinitis, Allergic, Perennial / Q000097:blood / Q000201:enzymology*
D012333:RNA, Messenger; D009569:Nitric Oxide; C496319:NOS2 protein, human; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II