[Effect of staphylococcal enterotoxin B on ion electrophysiology and permeability in rabbit maxillary sinus epithelium].
OBJECTIVE
To study the barrier role of staphylococcal enterotoxin B (SEB) in rabbit maxillary sinus mucosa.


METHODS
Rabbit maxillary sinus mucosal membrane was dissected under a surgical microscope. The short-circuit current (Isc), conductance (G) and its permeability to horseradish peroxidase (HRP) were evaluated by employing Ussing chamber technique.


RESULTS
The stimulation of SEB enhanced Isc, G and its permeability to HRP of sinus epithelial barrier. The same results were obtained by applying tumor necrosis factor alpha (TNF-alpha) to rabbit maxillary sinus. Increase in TNF-alpha level in sinus mucosa was observed by the stimulation of SEB. Those pathophysiological abnormalities of sinus mucosa were abolished by pretreatment with anti-TNF-alpha antibody.


CONCLUSIONS
The SEB has potential effects on rabbit maxillary sinus mucosal barrier, activates the immune cells in the mucosa, and plays certain roles in maxillary sinusitis.
Liu
Tao
T
Department of Otorhinolaryngology, First Hospital, Shanxi Medical University, Taiyuan 030001, China. lqy90326@public.ty.sx.cn
Yang
Pingchang
P
Wang
Binquan
B
Huangfu
Hui
H
Zhang
Nianzu
N
Xia
Lijun
L
D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects*; D004594:Electrophysiology; D004768:Enterotoxins / Q000494:pharmacology*; D008443:Maxillary Sinus / Q000187:drug effects* / Q000502:physiology; D009092:Mucous Membrane / Q000187:drug effects*; D011817:Rabbits; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D004768:Enterotoxins; D014409:Tumor Necrosis Factor-alpha; C031818:enterotoxin B, staphylococcal