Xenorhabdus nematophila requires an intact iscRSUA-hscBA-fdx operon to colonize Steinernema carpocapsae nematodes.
An insertion between iscA and hscB of the Xenorhabdus nematophila iscRSUA-hscBA-fdx locus, predicted to encode Fe-S assembly machinery, prevented colonization of Steinernema carpocapsae nematodes. The insertion disrupted cotranscription of iscA and hscB, but did not reduce hscBA expression, suggesting that X. nematophila requires coordinated expression of the isc-hsc-fdx locus for colonization.
Martens
Eric C
EC
Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Gawronski-Salerno
Joseph
J
Vokal
Danielle L
DL
Pellitteri
Molly C
MC
Menard
Megan L
ML
Goodrich-Blair
Heidi
H
D000818:Animals; D005288:Ferredoxins; D006360:Heat-Shock Proteins; D007501:Iron / Q000378:metabolism; D018832:Molecular Chaperones; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D016366:Open Reading Frames; D009876:Operon / Q000502:physiology*; D017168:Rhabditida / Q000382:microbiology*; D013455:Sulfur / Q000378:metabolism; D014157:Transcription Factors; D020593:Xenorhabdus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
C081714:2Fe-2S ferredoxin; D005288:Ferredoxins; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D014157:Transcription Factors; D013455:Sulfur; D007501:Iron