[Effect of aquaporin-1 and sodium channel on pleural fluid transport in mice].
OBJECTIVE
To investigate the effect of sodium channel and aquaporin-1 on pleural fluid transport.


METHODS
Mice were briefly anesthetized, and the pleural space was infused with 0.25 ml of hyperosmolar or isosmolar solution, with terbutaline or amiloride in instilled solution. The mice were treated with either intraperitoneal dexamethasone or intravenous HgCl(2). Mice were euthanized at specified times for measurement of pleural fluid osmolality, volume and (125)I-album in concentration. The rate of pleural fluid filtration and clearance was calculated, which was used to indicate the effect of sodium channel regulator (amiloride and terbutaline) and aquaporin regulator (dexamethasone and HgCl(2)) on pleural fluid transport.


RESULTS
Amiloride decreased the osmotically driven water transport and isosmolar fluid clearance in pleural space (P < 0.05). Terbutaline increased osmotically driven water transport and isosmolar fluid clearance in pleural space (P < 0.05). HgCl(2) decreased, while dexamethasone increased, the osmotically driven water transport in pleural space (P < 0.05). HgCl(2) and dexamethasone had no effect on pleural isosmolar fluid clearance (P > 0.05).


CONCLUSIONS
Sodium channel may play a role in osmotic and isosmolar fluid transport in pleural space. Aquaporin-1 may play a role in osmotic fluid transport in pleural space, while having no effect on isosmolar fluid clearance.
Jiang
Jin-jun
JJ
Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Bai
Chun-xue
CX
Hong
Qun-ying
QY
Song
Yuan-lin
YL
D000818:Animals; D051398:Aquaporin 1; D020346:Aquaporins / Q000494:pharmacology*; D001692:Biological Transport / Q000187:drug effects; D001826:Body Fluids / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D009997:Osmotic Pressure / Q000187:drug effects; D015222:Sodium Channels / Q000378:metabolism*
C496021:Aqp1 protein, mouse; D020346:Aquaporins; D015222:Sodium Channels; D051398:Aquaporin 1