title: Simultaneous determination of 4-hydroxy-2-alkenals, lipid peroxidation toxic products.
abstract: Lipid peroxidation has been associated with cell injury and possible carcinogenecity, for the products can damage cellular membrane and DNA. Among the products formed from lipid peroxidation, 4-hydroxy-2-alkenals are the major components and have been suggested to play a role in cellular toxicity. However, the difficulties of the quantification due to their strong reactivity during laboratory procedures hinder the exposure measurement of the compounds. A novel detection method based on the conversion of 4-hydroxy-2-alkenals to stable diols and to subsequent ortho-esters has been developed. Simultaneous detection in a sensitive single-ion monitoring mode by GC/MS was carried out, monitoring m/z 129, which was the common fragment ion for the ortho-esters derived from 4-hydroxy-2-alkenals. The proposed method was used to quantify 4-hydroxy-2-nonenal in oils and in oxidized arachidonic acid.
authors.affiliation: Department of Food and Nutrition, College of Human Ecology, Seoul National University, Kwanak-ku, Seoul 151-742, Korea.
authors.forename: J
authors.initials: J
authors.lastname: Surh
authors.forename: H
authors.initials: H
authors.lastname: Kwon
mesh_terms: D000447:Aldehydes / Q000032:analysis*; D016718:Arachidonic Acid / Q000737:chemistry; D004041:Dietary Fats / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D015227:Lipid Peroxidation; D014118:Toxins, Biological / Q000032:analysis*
chemical_list: D000447:Aldehydes; D004041:Dietary Fats; D014118:Toxins, Biological; D016718:Arachidonic Acid; C027576:4-hydroxy-2-nonenal