title: Influence of 6-week exercise training on erythrocyte and liver antioxidant defense in hyperinsulinemic rats.
abstract: High dosage of fructose in rats causes insulin resistance and hyperinsulinemia. This study investigates the effect of physical exercise on oxidant-antioxidant balance in rats fed a high fructose diet, which show characteristic features of insulin resistance. Products of lipid peroxidation and the activity of enzymic antioxidants namely superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase and glutathione reductase, in red blood cells (RBCs) and liver were assayed. Levels of non-enzymic antioxidants alpha-tocopherol and ascorbic acid and of protein and non-protein thiols were also determined. The levels of lipid peroxides, diene conjugates, lipofuscin and hydroperoxides were significantly higher in the liver of fructose-fed rats. The RBCs showed significantly higher susceptibility to H(2)O(2)-induced stress compared to control rats. Inadequate antioxidant system was noted in high fructose-fed rats. Physical training to these rats reversed the adverse effects, which could be important in alleviating the pathological consequences of insulin resistance.
authors.affiliation: Department of Biochemistry, Faculty of Science, Annamalai University, Annamalainagar, Tamil Nadu 608 002, India.
authors.forename: V
authors.initials: V
authors.lastname: Thirunavukkarasu
authors.forename: S D
authors.initials: SD
authors.lastname: Balakrishnan
authors.forename: M K
authors.initials: MK
authors.lastname: Ravichandran
authors.forename: C V
authors.initials: CV
authors.lastname: Anuradha
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001786:Blood Glucose / Q000032:analysis; D002478:Cells, Cultured / Q000187:drug effects; D004032:Diet; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology*; D005632:Fructose / Q000008:administration & dosage* / Q000633:toxicity; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007328:Insulin / Q000097:blood; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D010088:Oxidoreductases / Q000378:metabolism; D010805:Physical Conditioning, Animal; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D007328:Insulin; D005632:Fructose; D006861:Hydrogen Peroxide; D010088:Oxidoreductases