[Experimental study of apoptosis and proliferation of gastric mucosal cells in chronic atrophic gastritis].
OBJECTIVE
To study the relationship between long-term high-salt hot diet and chronic atrophic gastritis (CAG). The expression of apoptosis cells, proliferation cells, bcl-2 and Fas proteins were detected in the gastric mucosa of CAG rates induced by high-salt hot water.


METHODS
TUNEL technique and immunohistochemical method were used in the experiment. Flow cytometry method was used to detect the DNA content of apoptosis cells.


RESULTS
(1) The detection of apoptosis with TUNEL showed that the apoptosis cells in CAG rates induced by high-salt hot water were high. These cells could be seen in all layers of gastric mucosa, apoptosis index of model rates was higher than that of normal rates (P < 0.05). (2) The result of detecting apoptosis gene and proliferating cell nuclear antigen (PCNA) by immunohistochemical method showed that the expression of bcl-2, Fas and PCNA in the CAG rates induced by high-salt hot water was much higher than that of normal gastric mucosa (P < 0.05). The expression rates of bcl-2, Fas proteins were higher with time going on. After being given high-salt hot water intragastrically for 4, 8, 12 weeks, the bcl-2 protein expression rates were 12.5%, 16.7%, 76.5%, the Fas protein expression rates were 18.7%, 22.2% and 64.7%. (3) Flow cytometry technique showed that there was a second G(0)/G(1) peek, which was apoptosis cells peak, but was not seen in the normal gastric mucosa.


CONCLUSIONS
Abnormality of proliferation and apoptosis in gastric mucosa could be induced by high-salt hot water. bcl-2, Fas gene played an important role in this course.
Zhang
Ling-xia
LX
Department of Gastroenterology, Xi'an Central Hospital, Xi'an 710003, China. zlxblq@yahoo.cm.cn
Zhang
Li
L
Xu
Jun-rong
JR
Jia
Chang-he
CH
Zhang
Ning-xia
NX
Cao
Guang-zhou
GZ
D000818:Animals; D017209:Apoptosis; D002455:Cell Division; D004247:DNA / Q000032:analysis; D005753:Gastric Mucosa / Q000378:metabolism / Q000473:pathology*; D005757:Gastritis, Atrophic / Q000378:metabolism / Q000473:pathology*; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D019014:fas Receptor / Q000096:biosynthesis
D018809:Proliferating Cell Nuclear Antigen; D019253:Proto-Oncogene Proteins c-bcl-2; D019014:fas Receptor; D004247:DNA