The effect of cyclodextrins on the in vitro and in vivo properties of insulin-loaded poly (D,L-lactic-co-glycolic acid) microspheres.
In this work we describe the development and characterization of a new formulation of insulin (INS). Insulin was complexed with cyclodextrins (CD) in order to improve its solubility and stability being available as a dry powder, after encapsulation into poly (D,L-lactic-co-glycolic acid) (PLGA) microspheres. The complex INS : CD was encapsulated into microspheres in order to obtain particles with an average diameter between 2 and 6 microm. This system was able to induce significant reduction of the plasma glucose level in two rodent models, normal mice and diabetic rats, after intratracheal administration.
Rodrigues Júnior
José Maciel
JM
Laboratory of Pharmaceutical Technology, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. jmrj@rdbiotec.com.br
de Melo Lima
Karla
K
de Matos Jensen
Carlos Eduardo
CE
de Aguiar
Marta Maria Gontijo
MM
da Silva Cunha Júnior
Armando
A
D000818:Animals; D003505:Cyclodextrins / Q000737:chemistry*; D004337:Drug Carriers; D066298:In Vitro Techniques; D007328:Insulin / Q000008:administration & dosage / Q000138:chemical synthesis*; D019344:Lactic Acid / Q000737:chemistry*; D008297:Male; D051379:Mice; D008863:Microspheres; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry*; D011208:Powders; D051381:Rats; D017208:Rats, Wistar
D003505:Cyclodextrins; D004337:Drug Carriers; D007328:Insulin; D011108:Polymers; D011208:Powders; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid