title: Sodium-inorganic phosphate cotransporter NaPi-IIb in the epididymis and its potential role in male fertility studied in a transgenic mouse model.
abstract: Analysis by cDNA microarrays showed that in the murine epididymis, NaPi-IIb was the predominantly expressed epithelial isoform of the sodium-inorganic phosphate cotransporter and was markedly overexpressed in the proximal region in the infertile knockout (KO) compared to the fertile heterozygous (HET) c-ros transgenic mouse. The apparent up-regulation in the KO mouse confirmed by Northern and Western blot analyses could be explained by the absence of NaPi-IIb from the initial segment of the HET epididymis, as revealed by immunohistochemistry, and its presence on the epithelial brush border throughout the proximal epididymis of KO mice, where differentiation of the initial segment fails to occur. Both NaPi-IIb mRNA and protein were scarce or absent from the cauda epididymidis of both genotypes. A high content of inorganic phosphate was measured enzymatically in the HET cauda luminal fluid, with a 27% decrease in the KO mice. This decrease, presumably from a greater reabsorption of inorganic phosphate, particularly in the initial part of the KO epididymis, may disturb the normal process of sperm maturation in these infertile males. By contrast, no apparent consequences were observed for the transport of Na+ and Ca2+, the concentrations of which (approximately 26 mM and approximately 30 microM, respectively) were measured by microelectrodes to be identical in the caudal fluid from both genotypes.
authors.affiliation: Institute of Reproductive Medicine of the University, Münster, Germany.
authors.forename: Yaoxian
authors.initials: Y
authors.lastname: Xu
authors.forename: Ching-Hei
authors.initials: CH
authors.lastname: Yeung
authors.forename: Iwan
authors.initials: I
authors.lastname: Setiawan
authors.forename: Cosmina
authors.initials: C
authors.lastname: Avram
authors.forename: Jurg
authors.initials: J
authors.lastname: Biber
authors.forename: Andrea
authors.initials: A
authors.lastname: Wagenfeld
authors.forename: Florian
authors.initials: F
authors.lastname: Lang
authors.forename: Trevor G
authors.initials: TG
authors.lastname: Cooper
mesh_terms: D000818:Animals; D001826:Body Fluids / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D004822:Epididymis / Q000502:physiology*; D005260:Female; D005298:Fertility / Q000502:physiology*; D007248:Infertility, Male / Q000235:genetics; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D020411:Oligonucleotide Array Sequence Analysis; D010710:Phosphates / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics; D012313:RNA / Q000032:analysis / Q000235:genetics; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics; D012964:Sodium / Q000378:metabolism; D050608:Sodium-Phosphate Cotransporter Proteins; D050614:Sodium-Phosphate Cotransporter Proteins, Type IIb; D013094:Spermatozoa / Q000378:metabolism; D027981:Symporters / Q000502:physiology*
chemical_list: D010710:Phosphates; D011518:Proto-Oncogene Proteins; C493558:Slc34a2 protein, mouse; D050608:Sodium-Phosphate Cotransporter Proteins; D050614:Sodium-Phosphate Cotransporter Proteins, Type IIb; D027981:Symporters; D012313:RNA; D012964:Sodium; D020794:Receptor Protein-Tyrosine Kinases; C483813:Ros1 protein, mouse; D002118:Calcium