title: Systemic arteriovenous fistula leads to pulmonary artery remodeling and abnormal vasoreactivity in the fetal lamb.
abstract: Several cases of systemic arteriovenous fistula diagnosed in the human fetus have been associated with the postnatal development of persistent pulmonary hypertension. The aim of this study was to determine the effects of a prenatally created systemic arteriovenous fistula on the structure and reactivity of the pulmonary circulation in the fetal lamb. A fistula between the jugular vein and carotid artery was created in fetal lambs at 119-124 days of gestation. At delivery (134-139 days), left pulmonary artery (LPA) pressure was increased in the fistula group (n = 12) compared with controls (n = 11, P < 0.01). The pulmonary vascular resistance was significantly higher in the fistula group (P < 0.05), whereas mean LPA blood flow was not statistically different between the two groups. Morphometric analysis of the pulmonary vascular bed revealed an increase in the number of peripheral muscular arteries, together with an increase in pulmonary arterial medial thickness in the fistula group. There was no difference in the relative number or size of intraacinar arteries. In vitro organ bath studies on pulmonary arterial rings showed impaired endothelium-dependent relaxation in the fistula group compared with controls. However, endothelial nitric oxide synthase protein expression was similar in both groups, whereas endothelium-independent relaxation to sodium nitroprusside was greater in the fistula group compared with controls. A systemic arteriovenous fistula leads to both structural and functional alteration of the pulmonary vasculature, which might lead to the development of persistent pulmonary hypertension after birth.
authors.affiliation: Maternité, Hôpital Necker-Enfants Malades, 149 rue de Sèvres, 75015 Paris, France. ipp.jouannic@free.fr
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Jouannic
authors.forename: Régine
authors.initials: R
authors.lastname: Roussin
authors.forename: Alison A
authors.initials: AA
authors.lastname: Hislop
authors.forename: Sophie
authors.initials: S
authors.lastname: Lanone
authors.forename: Jelena
authors.initials: J
authors.lastname: Martinovic
authors.forename: Jorge
authors.initials: J
authors.lastname: Boczkowski
authors.forename: Yves
authors.initials: Y
authors.lastname: Dumez
authors.forename: A Tuan
authors.initials: AT
authors.lastname: Dinh-Xuan
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001164:Arteriovenous Fistula / Q000473:pathology / Q000503:physiopathology*; D015153:Blotting, Western; D000001:Calcimycin / Q000494:pharmacology; D005260:Female; D006976:Hypertension, Pulmonary / Q000473:pathology / Q000503:physiopathology*; D007476:Ionophores / Q000494:pharmacology; D007537:Isometric Contraction / Q000187:drug effects; D019001:Nitric Oxide Synthase / Q000032:analysis; D052250:Nitric Oxide Synthase Type III; D011247:Pregnancy; D011651:Pulmonary Artery / Q000002:abnormalities / Q000503:physiopathology*; D011652:Pulmonary Circulation / Q000502:physiology*; D020545:Respiratory Mucosa / Q000201:enzymology / Q000473:pathology / Q000503:physiopathology; D012756:Sheep; D014664:Vasodilation / Q000187:drug effects; D014665:Vasodilator Agents / Q000494:pharmacology
chemical_list: D007476:Ionophores; D014665:Vasodilator Agents; D000001:Calcimycin; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III; D000109:Acetylcholine