title: Cellular uptake and intracellular fate of antisense oligonucleotides.
abstract: Antisense oligonucleotides and short interfering RNAs are routinely used for gene function analysis and are being developed for clinical applications. The mechanism underlying internalization of free oligonucleotides into cells is poorly understood and inefficient in most cases. Antisense oligonucleotide delivery into ex vivo cells is routinely improved by the addition of cationic lipids. New chemical modifications and vectors allowing improved cellular delivery in vivo are being developed.
authors.affiliation: Molecular Genetics Institute, UMR 5124 CNRS, Université Montpellier 2, 1919 route de Mende, 34293 Montpellier, France.
authors.forename: Alain R
authors.initials: AR
authors.lastname: Thierry
authors.forename: Eric
authors.initials: E
authors.lastname: Vives
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Richard
authors.forename: Paul
authors.initials: P
authors.lastname: Prevot
authors.forename: Camille
authors.initials: C
authors.lastname: Martinand-Mari
authors.forename: Ian
authors.initials: I
authors.lastname: Robbins
authors.forename: Bernard
authors.initials: B
authors.lastname: Lebleu
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D005822:Genetic Vectors; D006801:Humans; D016376:Oligonucleotides, Antisense / Q000493:pharmacokinetics* / Q000627:therapeutic use
chemical_list: D016376:Oligonucleotides, Antisense