[Anti-emetic effect of ethanol extract from "Wuzhuyu broth"].
OBJECTIVE
To investigate the anti-emetic effect of ethanol extract from "WuZhuYu broth" and its mechanism.


METHODS
Three experiments were carried out test which extract has anti-emetic activity, such as CuSO4-induced pigeon's emetic response, gastric emptying in mice and ACh-induced or 5-HT-induced contraction in vitro gastric muscle in rats. Meanwhile, effect of anti-emetic extract on concentration-response curve to ACh, 5-HT, histamine was investigated.


RESULTS
50% ethanol extract and 70% ethanol extract were identified as having significantly stronger anti-emetic activities with little side effect, which showed the significant effect on concentration-response curve to ACh, 5-HT, histamine.


CONCLUSIONS
50% ethanol extract and 70% ethanol extract contain more anti-emetic fractions, more anti-emetic fractions can be gained at the concentrations of 50% and 70% ethanol; the mechanism of anti-emetic effect is related to its antagonism to the receptors of ACh, 5-HT, histamine.
Zhang
Ting
T
Shanghai Institute of Materia Medica, Shanghai 200031, China.
Wang
Min-wei
MW
Chen
Si-wei
SW
D000818:Animals; D000932:Antiemetics / Q000627:therapeutic use*; D010856:Columbidae; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000627:therapeutic use*; D032086:Evodia / Q000737:chemistry*; D005260:Female; D005746:Gastric Emptying / Q000187:drug effects; D008297:Male; D051379:Mice; D009119:Muscle Contraction / Q000187:drug effects; D008517:Phytotherapy; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D014839:Vomiting / Q000188:drug therapy*
D000932:Antiemetics; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; C478371:wuzhuyu broth