[Bilirubin induced apoptosis of human neuroblastoma cell line SH-SY5Y and affected the mitochondrial membrane potential].
OBJECTIVE
To study whether bilirubin induces apoptosis of human neuroblastoma cell line SH-SY5Y and affects the mitochondrial membrane potential.


METHODS
SH-SY5Y cells were exposed to bilirubin, then morphological changes of nucleus were assayed by Hoechst 33258 staining. The percentage of apoptotic cells was measured by flow cytometry. The mitochondrial membrance potential(MMP) was detected by flow cytometry through Rhodamine123 staining.


RESULTS
MMP of SH-SY5Y cells significantly decreased from (16.6 +/- 1.556) U to (7.11 +/- 0.701) U (P < 0.01) after exposed to 0.02 g/L bilirubin for 1 h. The percentage of apoptotic cells was 19.4% after exposed for 3 h, and significantly increased to 76.4% after 4 h, but typical apoptotic nuclear changes occured after 6 h.


CONCLUSIONS
Bilirubin could early cause disruption of MMP, then induce the apoptosis of SH-SY5Y cells.
Han
Zhang
Z
Department of Otorhinolaryngology, Peking Union Medical College Hospital, PUMC & CAMS, Beijing 100730, China.
Hu
Ping
P
Ni
Daofeng
D
D017209:Apoptosis / Q000187:drug effects*; D001663:Bilirubin / Q000494:pharmacology*; D045744:Cell Line, Tumor; D006801:Humans; D008564:Membrane Potentials / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects* / Q000502:physiology; D009447:Neuroblastoma / Q000473:pathology*
D001663:Bilirubin