Intranuclear Ca2+ signals within individual nuclear lobes of neutrophils.
Cytosolic free Ca(2+)and intranuclear Ca(2+)behave similarly in human neutrophils. However, conventional laser scanning at 350 ms/frame resolution at lower than physiological temperatures demonstrates that (i) the nuclear fluo3-Ca(2+)signal persists longer than the cytosolic signal in some (but not all) nuclear lobes, (ii) the neutrophil nuclear membrane and fine inter-lobe filaments present barriers to diffusion of fluo3-Ca(2+)and lucifer yellow, and (iii) the diffusion barrier correlates with condensed chromosomal material on the nuclear envelope and blockage of the movement of fluo3-Ca(2+)into individual nuclear lobes.
Hodges
Robert
R
Molecular Signalling Group, University Department of Surgery, University of Wales College of Medicine, Heath Park, CF14 4XN, Cardiff, UK.
Laffafian
Iraj
I
Hallett
Maurice B
MB
D020013:Calcium Signaling / Q000502:physiology*; D002467:Cell Nucleus / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D042581:Intranuclear Space / Q000378:metabolism / Q000502:physiology; D009504:Neutrophils / Q000378:metabolism* / Q000502:physiology; D009685:Nuclear Envelope / Q000378:metabolism / Q000502:physiology