title: A fluorescent high-throughput assay for double-stranded DNA: the RediPlate PicoGreen assay.
abstract: The fluorescent PicoGreen reagent for detection and quantitation of double-stranded DNA has been adapted for high-throughput screening: the RediPlate PicoGreen double-stranded DNA assay format. In the RediPlate PicoGreen assay format, the PicoGreen reagent is predistributed and co-dried into either 96- or 384-well microplates with the excipient trehalose. The user resuspends the dried reagents upon adding DNA, and measures the resulting fluorescence after a five minute incubation. Replicate fluorescence measurements on nominally identical wells have less than a 5% coefficient of variation. The assay is linear from 5 to 500 ng/ml DNA in a 200 micro l volume. The RediPlate PicoGreen assay format retains the advantages of the original PicoGreen reagent - sensitivity, speed, and specificity - but in a high-throughput format.
authors.affiliation: Probes, Inc., 29851 Willow Creek Road, Eugene, OR 97402, USA. rob.batchelor@probes.com
authors.forename: Robert
authors.initials: R
authors.lastname: Batchelor
authors.forename: David
authors.initials: D
authors.lastname: Hagen
authors.forename: Iain
authors.initials: I
authors.lastname: Johnson
authors.forename: Joseph
authors.initials: J
authors.lastname: Beechem
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D005470:Fluorometry; D009930:Organic Chemicals; D015203:Reproducibility of Results
chemical_list: D004279:DNA, Viral; D005456:Fluorescent Dyes; D009930:Organic Chemicals; C099476:PicoGreen