[Floating culture of human nasal glandular cells].
OBJECTIVE
To establish a floating cultural model of human nasal glandular (HNG) cells.


METHODS
HNG cells were cultured on floating collagen gels.


RESULTS
Cultured HNG cells incubated with monoclonal antibody for cytokeratin stained positively. After two-week culture, the cells stained blue by AB-PAS staining. Transmission electron microscopy showed that the cells exhibited numerous microvilli on their surfaces and had numerous secretary granules. They were well polarized and interconnected by junctional complexes. Cultured HNG cells possessed typical characteristics of normal epithelium.


CONCLUSIONS
The floating cultural model of HNG was successfully established.
Guo
Y
Y
Department of Otorhinolaryngology Head and Neck Surgery, Third Clinical College of Bethune University of Medical Sciences, Changchun 130031.
Yang
Z
Z
Bu
G
G
D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002478:Cells, Cultured; D003094:Collagen; D003470:Culture Media; D005782:Gels; D006801:Humans; D009297:Nasal Mucosa / Q000166:cytology* / Q000648:ultrastructure
D003470:Culture Media; D005782:Gels; D003094:Collagen