Improving heterologous gene expression in Aspergillus niger by introducing multiple copies of protein-binding sequence containing CCAAT to the promoter.
OBJECTIVE
To increase the expression level of heterologous gene in Aspergillus niger.


RESULTS
Inserting multiple copies of an activator protein-binding site originally encoded in the cis-regulatory region of A. niger glucoamylase gene (glaA), CCAAT-containing sequence, into the promoter of an expression plasmid greatly enhanced the production of heterologous protein.


CONCLUSIONS
The promoter activity was increased by the introduction of the CCAAT-containing sequence resulting in increased gene expression.


CONCLUSIONS
This study provides evidence that the CCAAT-containing region of the glaA promoter of A. niger is a binding site for a positive transcriptional factor and demonstrates the possibility for improving the expression of both heterologous and endogenous genes in Aspergillus.
Liu
L
L
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China.
Liu
J
J
Qiu
R X
RX
Zhu
X G
XG
Dong
Z Y
ZY
Tang
G M
GM
D001234:Aspergillus niger / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D018628:Gene Dosage; D015966:Gene Expression Regulation, Fungal; D005087:Glucan 1,4-alpha-Glucosidase / Q000737:chemistry* / Q000235:genetics; D006454:Hemoglobins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011995:Recombination, Genetic; D014170:Transformation, Genetic; D054793:Truncated Hemoglobins
D001426:Bacterial Proteins; D006454:Hemoglobins; D054793:Truncated Hemoglobins; C082613:hemoglobin protein, Vitreoscilla; D005087:Glucan 1,4-alpha-Glucosidase