Quality assessment of seven types of fresh-frozen plasma leucoreduced by specific plasma filtration.
OBJECTIVE
A study was undertaken to determine plasma quality after specific filtration.


METHODS
Seven types of plasma were tested, after filtration of plasma from filtered or non-filtered whole blood. Leucocyte counting was carried out after a 30-fold concentration of the sample. Twenty-nine parameters (including coagulation testing, proteins, coagulation factors and activation markers) were measured before and after filtration, and after 6 months of storage.


RESULTS
After specific plasma filtration, the average residual leucocyte counts were less than 2250/l. In spite of small statistically significant changes in proteins, coagulation factors and complement activation, this study showed that plasma filtration did not alter plasma quality. After 6 months of storage at -30 degrees C, factor VIII recovery varied between 91 and 109%. Haemostasis parameters and activation markers remained within the normal range.


CONCLUSIONS
Specific plasma filtration reduced the leucocyte number to < 104 leucocytes/l. The quality of plasma was not altered by the additional step of specific plasma filtration.
Chabanel
A
A
Etablissement Français du Sang, Paris, France. anne.chabanel@efs.sante.fr
Sensebé
I
I
Masse
M
M
Maurel
J P
JP
Plante
J
J
Hivet
D
D
Kannengiesser
C
C
Naegelen
C
C
Joussemet
M
M
Marchesseau
B
B
Rasongles
P
P
Proust
F
F
David
C
C
Montembault
A M
AM
Bergeat
P
P
D015415:Biomarkers / Q000097:blood; D001780:Blood Coagulation Tests; D001793:Blood Preservation; D002469:Cell Separation / Q000379:methods; D005169:Factor VIII / Q000032:analysis; D005374:Filtration; D006487:Hemostasis; D006801:Humans; D007958:Leukocyte Count; D007962:Leukocytes; D010949:Plasma; D011785:Quality Assurance, Health Care
D015415:Biomarkers; D005169:Factor VIII