Inhibition of nitric oxide production on LPS-activated macrophages by kazinol B from Broussonetia kazinoki.
The ethyl acetate soluble fraction of Broussonetia kazinoki at 50 microg/ml showed a significant inhibition (78.2%) of nitric oxide (NO) in lipopolysaccharide activated macrophages. Kazinol B, an isoprenylated flavan, was identified as the active principle. It inhibits the NO synthesis with an IC(50) of 21.6 microM.
Ryu
Jae-Ha
JH
College of Pharmacy, Sookmyung Women's University, Seoul 140-742, South Korea. ryuha@sdic.sookmyung.ac.kr
Ahn
Hanna
H
Jin Lee
Hwa
H
D000818:Animals; D015153:Blotting, Western; D031623:Broussonetia; D004591:Electrophoresis, Polyacrylamide Gel; D005419:Flavonoids / Q000008:administration & dosage / Q000494:pharmacology*; D005638:Fruit; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D019001:Nitric Oxide Synthase / Q000096:biosynthesis*; D052247:Nitric Oxide Synthase Type II; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology; D018515:Plant Leaves
D005419:Flavonoids; D008070:Lipopolysaccharides; D010936:Plant Extracts; C117415:kazinol B; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse