title: Temporal and spatial expression of fibroblast growth factor receptor 4 isoforms in murine tissues.
abstract: Fibroblast growth factor receptors (FGFRs) undergo highly regulated spatial and temporal changes of expression during development. This study describes the use of quantitative reverse transcriptase-polymerase chain reaction and immunochemistry to assess the changes in expression of FGFR4 as compared to its FGFR4-17a and -17b isoforms in mouse tissues, from early embryogenesis through to adulthood. Compared to FGFR4, the expression of the isoforms is more restricted at all developmental stages tested. The reverse transcriptase-polymerase chain reaction demonstrated that FGFR4 is expressed in more tissue types than either of its isoforms: it was found predominantly in lung, liver, brain, skeletal muscle and kidney, whereas the FGFR4-17a form was detected in lung and skeletal muscle, and the FGFR4-17b form only in lung, liver, skeletal muscle and kidney. Immunohistochemistry confirmed strong FGFR4-17b expression in the postnatal lung. When combined, the results suggest that FGFR4 variants play important roles particularly in lung and skeletal muscle development.
authors.affiliation: School of Biomedical Sciences, University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: Simon M
authors.initials: SM
authors.lastname: Cool
authors.forename: Rosamond E
authors.initials: RE
authors.lastname: Sayer
authors.forename: Walter R
authors.initials: WR
authors.lastname: van Heumen
authors.forename: James O
authors.initials: JO
authors.lastname: Pickles
authors.forename: Victor
authors.initials: V
authors.lastname: Nurcombe
mesh_terms: D000818:Animals; D001921:Brain / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D005260:Female; D006321:Heart / Q000196:embryology / Q000254:growth & development; D007150:Immunohistochemistry; D007668:Kidney / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D008099:Liver / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D008168:Lung / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D051379:Mice; D018482:Muscle, Skeletal / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism; D011247:Pregnancy; D020033:Protein Isoforms; D012333:RNA, Messenger / Q000032:analysis; D051499:Receptor, Fibroblast Growth Factor, Type 4; D017468:Receptors, Fibroblast Growth Factor / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D020033:Protein Isoforms; D012333:RNA, Messenger; D017468:Receptors, Fibroblast Growth Factor; C496372:Fgfr4 protein, mouse; D051499:Receptor, Fibroblast Growth Factor, Type 4