title: [Molecular authentication of Dendrobium chrysanthum from its allied species of Dendrobium].
abstract: OBJECTIVE
  To define molecular characters to distinguish D. chrysanthum from its allied species D. primulinum, D. lituiflorum, D. aphyllum, D. crepidatum.
  
  
  METHODS
  The molecular characteristics of D. chrysanthum and its allied species were compared. The sequences of rDNA ITS regions were exploited to explore the evidence for authentication D. chrysanthum and its allied species.
  
  
  RESULTS
  Although the morphological difference was slight, the sequence difference of ITS regions among five rDNAs was obvious and stable. Fifteen sites of ITS region were defined as DNA character to identify D. chrysanthum from the other four allied species.
  
  
  CONCLUSIONS
  The difference of rDNA ITS sequences can be used to authenticate accurately D. chrysanthum from three allied species of Dendrobium.
authors.affiliation: Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210038, Jiangsu, China. dingxynj@263.net
authors.forename: Xiao-yu
authors.initials: XY
authors.lastname: Ding
authors.forename: Luo-san
authors.initials: LS
authors.lastname: Xu
authors.forename: Zheng-tao
authors.initials: ZT
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
authors.forename: Kai-ya
authors.initials: KY
authors.lastname: Zhou
mesh_terms: D001483:Base Sequence; D018744:DNA, Plant / Q000235:genetics*; D031667:Dendrobium / Q000145:classification / Q000235:genetics*; D004340:Drug Contamination; D008969:Molecular Sequence Data; D010946:Plants, Medicinal / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D018744:DNA, Plant