Differential effects of vegetable-derived indoles on the induction of quinone reductase in hepatoma cells.
The increased expression of quinone reductase (QR) has been associated with anticarcinogenic processes. The aim of this study was to explore the roles of the cruciferous vegetable-derived indoles, indole-3-carbinol (I3C) and indolo[3.2-b]carbazole (ICZ), on the regulation of QR in both murine (Hepa-1) and human (HepG2) hepatoma cells. The results indicate that ICZ enhanced QR activity in both Hepa-1 and HepG2 cells, whereas its parent compound. I3C, had no significant effect on the induction of QR. Moreover, the ICZ-induced QR activity showed a higher response and expressed a more-significant dose-response in Hepa-1 cells. QR mRNA expression as analyzed by RT-PCR demonstrated a pattern similar to that of the enzyme activity. in conclusion, I3C did not show an enhancement effect on OR activity, but its acidic derivative, ICZ, increased the expression of QR mRNA, which then caused the augmentation of QR activity in Hepa-1 and HepG2 cells.
Chen
Yue-Hwa
YH
Graduate Institute of Nutrition and Health Sciences, Taipei Medical University, 250 Wu-Hsing Street, Taipei, Taiwan 110, ROC. yuehwa@tmu.edu.tw
Yang
Darlene
D
D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D001937:Brassica / Q000737:chemistry; D002227:Carbazoles / Q000494:pharmacology*; D004790:Enzyme Induction / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006801:Humans; D007211:Indoles / Q000494:pharmacology*; D008114:Liver Neoplasms, Experimental / Q000201:enzymology; D051379:Mice; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D016588:Anticarcinogenic Agents; D002227:Carbazoles; D007211:Indoles; D012333:RNA, Messenger; C075954:indolo(3,2-b)carbazole; C016517:indole-3-carbinol; D016660:NAD(P)H Dehydrogenase (Quinone)