title: Induction of MafBx and Murf ubiquitin ligase mRNAs in rat skeletal muscle after LPS injection.
abstract: MafBx and Murf are two new rat E3 ubiquitin ligases induced in muscle atrophy. Our goal was to investigate whether lipopolysaccharide (LPS) injection, a model of muscle catabolism, is associated with increased expression of MafBx and Murf. LPS (750 microg/100 g body weight) induces MafBx and Murf mRNA (respectively, 23-fold and 33-fold after 12 h; P<0.001). A transient induction of tumor necrosis factor-alpha mRNA (21-fold; P<0.001 at 3 h) and a decrease of insulin like growth factor-I mRNA (50%; P<0.001 at 6 h), two potential regulators of the ubiquitin-proteasome system were also demonstrated. In summary, MafBx and Murf mRNA are up-regulated in response to LPS and might play a role in the muscle proteolysis observed.
authors.affiliation: Unité de Diabétologie et Nutrition, Université catholique de Louvain, B-1200 Brussels, Belgium.
authors.forename: Mischaël J M
authors.initials: MJ
authors.lastname: Dehoux
authors.forename: Ronald P
authors.initials: RP
authors.lastname: van Beneden
authors.forename: Laura
authors.initials: L
authors.lastname: Fernández-Celemín
authors.forename: Pascale L
authors.initials: PL
authors.lastname: Lause
authors.forename: Jean-Paul M
authors.initials: JP
authors.lastname: Thissen
mesh_terms: D000818:Animals; D001835:Body Weight; D008025:Ligases / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000378:metabolism*; D009124:Muscle Proteins / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D044843:SKP Cullin F-Box Protein Ligases; D013997:Time Factors; D000070584:Tripartite Motif Proteins; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases; D015854:Up-Regulation
chemical_list: D008070:Lipopolysaccharides; D009124:Muscle Proteins; D012333:RNA, Messenger; D000070584:Tripartite Motif Proteins; D014409:Tumor Necrosis Factor-alpha; D012313:RNA; C440907:FBXO32 protein, human; C486692:Fbxo32 protein, rat; D044843:SKP Cullin F-Box Protein Ligases; C440908:TRIM63 protein, human; C499086:Trim63 protein, rat; D044767:Ubiquitin-Protein Ligases; D008025:Ligases