title: Molecular taxonomy of dermatophytes and related fungi by chitin synthase 1 (CHS1) gene sequences.
abstract: In the present study, the nucleotide sequences of the CHS1 gene from dermatophytes and related fungi in the genera Chrysosporium, Epidermophyton, Microsporum and Trichophyton were investigated using molecular methods. About 440-bp genomic DNA fragments of the CHS1 gene from 21 species were amplified by polymerase chain reaction (PCR) and sequenced. The CHS1 nucleotide sequences of these fungi showed more than 83% similarity. The molecular taxonomy of the CHS1 gene sequences revealed that Microsporum was genetically distinct from Chrysosporium and Trichophyton, as classified by morphological characteristics.
authors.affiliation: Department of Pathobiology, Nihon University School of Veterinary Medicine, 1866, Kameino, Fujisawa, Kanagawa 252-8510, Japan.
authors.forename: Asuka
authors.initials: A
authors.lastname: Hirai
authors.forename: Rui
authors.initials: R
authors.lastname: Kano
authors.forename: Yuka
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Shinichi
authors.initials: S
authors.lastname: Watanabe
authors.forename: Atsuhiko
authors.initials: A
authors.lastname: Hasegawa
mesh_terms: D003883:Arthrodermataceae / Q000145:classification* / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D002687:Chitin Synthase / Q000235:genetics* / Q000378:metabolism; D002912:Chrysosporium / Q000201:enzymology / Q000235:genetics; D004821:Epidermophyton / Q000201:enzymology / Q000235:genetics; D006801:Humans; D008864:Microsporum / Q000201:enzymology / Q000235:genetics; D003904:Mitosporic Fungi / Q000145:classification* / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014249:Trichophyton / Q000201:enzymology / Q000235:genetics
chemical_list: D002687:Chitin Synthase