Effect of intestinal inflammation on capsaicin-sensitive afferents in the ileum of Schistosoma mansoni-infected mice.
In the present study, the effect of intestinal schistosomiasis on the extrinsic sensory innervation of the murine ileum was investigated. Immunocytochemical techniques to localize calcitonin gene-related peptide (CGRP), substance P (SP), and vanilloid receptor 1 (VR1) were combined with retrograde tracing techniques and capsaicin treatment. Neurochemical characterization of extrinsic primary afferent neurons (EPANs) in normal and capsaicin-treated mice, revealed that CGRP and VR1, but not SP, were expressed in extrinsic afferents. Immunocytochemical analysis using the above-mentioned antibodies yielded three different populations of neurons in both dorsal root and nodose ganglia, namely CGRP/--, SP/--, and CGRP/SP-expressing neurons. Retrograde tracing revealed that only CGRP/--expressing neurons projected to the ileum. Intestinal schistosomiasis resulted in an upregulation of the number of CGRP-immunoreactive (ir) nerve fibers in the lamina propria of the villi, coinciding with an increase in mucosal mast cells in acutely and chronically infected animals. In infected animals, mucosal mast cells were found closely associated with a dense mucosal CGRP-ir fiber network. Neonatal capsaicin treatment led to a 70% reduction in the number of mucosal mast cells. In conclusion, the present study provides evidence that CGRP is a valid marker for EPANs in the mouse ileum, which are involved in the recruitment of mucosal mast cells. Morphological evidence is provided of a neuroimmune interaction between mucosal mast cells and EPANs in schistosoma-infected mice.
De Jonge
Frederik
F
Laboratory of Cell Biology and Histology, Department of Biomedical Sciences, University of Antwerp (RUCA), Groenenborgerlaan 171, 2020 Antwerp, Belgium.
Van Nassauw
Luc
L
Adriaensen
Dirk
D
Van Meir
Frans
F
Miller
Hugh R P
HR
Van Marck
Eric
E
Timmermans
Jean-Pierre
JP
D000818:Animals; D000831:Animals, Newborn; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D002211:Capsaicin / Q000008:administration & dosage / Q000494:pharmacology; D004195:Disease Models, Animal; D017615:Enteric Nervous System / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D019084:Fluorescent Antibody Technique, Indirect; D005727:Ganglia, Spinal / Q000378:metabolism / Q000473:pathology; D007082:Ileum / Q000294:innervation / Q000469:parasitology / Q000473:pathology*; D007279:Injections, Subcutaneous; D007413:Intestinal Mucosa / Q000187:drug effects / Q000469:parasitology / Q000473:pathology; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000473:pathology; D051379:Mice; D009475:Neurons, Afferent / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D009620:Nodose Ganglion / Q000378:metabolism / Q000473:pathology; D010273:Parasitic Diseases, Animal / Q000378:metabolism / Q000473:pathology*; D011955:Receptors, Drug / Q000378:metabolism; D012555:Schistosomiasis mansoni / Q000378:metabolism / Q000473:pathology*; D013373:Substance P / Q000378:metabolism
D011955:Receptors, Drug; D013373:Substance P; D015740:Calcitonin Gene-Related Peptide; D002211:Capsaicin