Proteolytic activities in togwa, a Tanzanian fermented food.
Proteolytic activities were investigated in sorghum-based togwa prepared by natural fermentation and using starter cultures previously isolated from the native product, i.e., Lactobacillus brevis, Lactobacillus cellobiosus, Lactobacillus fermentum, Lactobacillus plantarum, Pediococcus pentosaceus, and Issatchenkia orientalis in coculture with either L. brevis or L. plantarum. Both proteinase and aminopeptidase activities were substantially higher in naturally fermented togwa than in those with starters (14-30%, 12-70%, respectively). A variable but substantial part of the proteinase activity followed the particulate fraction of togwa; aminopeptidase activity was mainly in that fraction. The breakdown of relatively high molecular mass protein (64 kDa) in togwa was detected by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE); the products were mainly in the 14-30 kDa range. Reversed-phase fast-protein liquid chromatography (RP-FPLC)-protein/peptide patterns changed during fermentation with some variation between togwa of different cultures. Supplementation of gruel with malt increased the concentration of total protein [from 9.5% to 11.0% (w/w) on dry weight basis)] and of most of the free amino acids. Fermentation had no effect on total protein content; however, the concentration of most of the amino acids was reduced, except for the proline content that increased. Natural fermentation also increased the concentration of glutamic acid and ornithine. Fermentation by P. pentosaceus increased aspartic acid, while L. cellobiosus, L. fermentum, and L. brevis in coculture with I. orientalis increased the concentration of citrulline.
Mugula
J K
JK
Department of Food Science, Agricultural University of Norway, PO Box 5036, N-1432 As, Norway. jmugula@yahoo.com
Sørhaug
T
T
Stepaniak
L
L
D000626:Aminopeptidases / Q000378:metabolism; D002853:Chromatography, Liquid; D018920:Coculture Techniques; D002523:Edible Grain / Q000201:enzymology / Q000382:microbiology*; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000378:metabolism*; D005285:Fermentation; D005511:Food Handling / Q000379:methods; D005516:Food Microbiology; D007778:Lactobacillus / Q000201:enzymology / Q000378:metabolism*; D008970:Molecular Weight; D010376:Pediococcus / Q000201:enzymology / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D004718:Saccharomycetales / Q000201:enzymology / Q000378:metabolism; D013636:Tanzania; D015003:Yeasts / Q000201:enzymology / Q000378:metabolism
D010940:Plant Proteins; D010450:Endopeptidases; D000626:Aminopeptidases