[The TNF-alpha-induced apoptosis of LA795 cells is mediated by MAP kinase kinase 6].
OBJECTIVE
To study the molecular mechanism of apoptosis of lung cancer cells stimulated by tumor necrosis factor alpha for the discovery of potential methods of gene therapy of lung cancer.


METHODS
The apoptosis model of LA795 adenocarcinoma cells was performed. Recombinant adenovirus of MAP kinase kinase 6, and its constitutively active form and dominant negative form were prepared in 293 package cells. Protein kinase assay was used to detect the activity of MKK6 in cells.


RESULTS
TNF-alpha stimulation increased the activity of MKK6 in LA795 lung cancer cells. The stimulation of TNF-alpha induced apoptosis of LA795 cells significantly. The infection of recombinant virus of constitutive active form of MKK6 also induced apoptosis of LA795 cells significantly, while the infection of recombinant virus of dominant negative form of MKK6 blocked the apoptosis of LA795 cells apparently.


CONCLUSIONS
TNF-alpha induced apoptosis of LA795 cells is mediated by MKK6. It is possible to use MKK6 recombinant virus for gene therapy of lung cancer.
Liu
Ai-hua
AH
Department of Respiratory Diseases, Nanfang Hospital, The First Military Medical University, Guangzhou 510515, China.
Deng
Peng
P
Jiang
Yong
Y
D000230:Adenocarcinoma / Q000201:enzymology / Q000473:pathology*; D000256:Adenoviridae / Q000235:genetics; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000201:enzymology; D008175:Lung Neoplasms / Q000201:enzymology / Q000473:pathology*; D048669:MAP Kinase Kinase 6 / Q000378:metabolism* / Q000502:physiology; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D048669:MAP Kinase Kinase 6