Early changes in glucose and phospholipid metabolism following apoptosis induction by IFN-gamma/TNF-alpha in HT-29 cells.
The effects of apoptosis induction on glucose and phospholipid metabolite levels in cancer were studied using human colon adenocarcinoma cells (HT-29). Apoptosis was induced by co-incubation with 200 U/ml tumor necrosis factor (TNF)-alpha for 4, 8 or 15 h, after sensitization with 500 U/ml interferon (IFN)-gamma for 7 h. Perchloric acid extracts were analyzed by (1)H and (31)P nuclear magnetic resonance (NMR) spectroscopy. Significantly increased lactate and NTP (all nucleoside 5'-triphosphates) signals were detected 4 h after apoptosis-inducing IFN-gamma/TNF-alpha treatment, but not in cells which were TNF-alpha-treated without IFN-gamma preincubation. Simultaneous lactate and NTP changes, if confirmed in vivo, may serve as early, non-invasive markers of treatment response in some tumors.
Lutz
Norbert W
NW
Centre de Résonance Magnétique Biologique et Médicale, Faculté de Médecine, Marseille, France. nwlutz@u.arizona.edu
Tome
Margaret E
ME
Cozzone
Patrick J
PJ
D017209:Apoptosis; D016923:Cell Death; D016207:Cytokines / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D010743:Phospholipids / Q000378:metabolism*; D013997:Time Factors; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D016207:Cytokines; D010743:Phospholipids; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D005947:Glucose