[Detection of IgM-antibody to hepatitis G virus and its clinical significance].
OBJECTIVE
To provide a rapid and early serodiagnostic technique for the patients with hepatitis G virus(HGV).


METHODS
A capture ELISA was developed to detect anti-HGV IgM in sera using synthetic peptides of HGV NS3 NS5 gene regions conjugated with horseradish peroxidase.


RESULTS
This assay was not disturbed by competition of specific IgG or interfered by RF and did not cross-react with HAV, HBV, HCV, HEV, CMV and EBV. Among 46 sera of non-A-E hepatitis, 14 were positive for anti-HGVIgM, the positive rate was 30.43%, 6 was positive for HGV RNA the positive rate was 42.86%. 12 paired sera from acute stage of HGV patients were all positive by capture-ELISA.


CONCLUSIONS
This method is sensitive, specific, rapid and stable for detecting anti-HGV IgM, and is useful in the early diagnosis of HGV infection.
Liu
H
H
Department of Virology, 302 Hospital of PLA, Beijing 100039.
Mao
P
P
Hong
S
S
D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D018178:Flaviviridae Infections / Q000276:immunology* / Q000821:virology; D029407:GB virus C / Q000276:immunology; D006508:Hepatitis Antibodies / Q000097:blood*; D006525:Hepatitis, Viral, Human / Q000276:immunology* / Q000821:virology; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood*
D006508:Hepatitis Antibodies; D007075:Immunoglobulin M