Flow cytometric evaluation of postmortem pulp DNA degradation.
During postmortem autolysis, cellular organelles and nuclear DNA break down into their constituent parts. DNA flow cytometric analysis was applied to study the denaturation of splenic cell DNA as a possible method for postmortem interval determination. DNA denaturation continued for 72 hours at a constant rate, with no intact DNA peaks thereafter. The value of using dental pulp tissue for flow cytometric determination of postmortem interval was investigated. The pulps of 57 routinely removed impacted third molars from patients 15 to 30 years of age were obtained. Pulp tissue was removed at 24, 48, 72, 96, 120, and 144 hours postextraction. Debris (degraded DNA) was defined as all signals left of the standardized mean 2n peak and expressed as a percentage of the total number of signals. In contrast to the splenic cell DNA, dental pulp tissue exhibited minimal DNA degradation by 144 hours postextraction, and no constant relation was found between time and DNA degradation during this time. In this study, pulp tissue was found to be unreliable to determine the early postmortem interval but might be of greater value in the later stages.
Boy
Sonja C
SC
Department of Oral Pathology and Oral Biology, School of Dentistry, University of Pretoria, Gauteng, South Africa. sboy@postillion.up.ac.za
Bernitz
Herman
H
Van Heerden
Willie F P
WF
D000293:Adolescent; D000328:Adult; D004247:DNA / Q000032:analysis*; D053938:DNA Fragmentation; D003782:Dental Pulp / Q000737:chemistry* / Q000473:pathology*; D005434:Flow Cytometry / Q000379:methods*; D005554:Forensic Medicine / Q000379:methods*; D006801:Humans; D008964:Molar, Third / Q000473:pathology; D010865:Pilot Projects; D011180:Postmortem Changes; D014095:Tooth, Impacted / Q000601:surgery
D004247:DNA