title: Production of vascular endothelial growth factor and angiogenic factor in human follicular fluid.
abstract: The aim of this study was to quantitate of vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), and angiogenin in follicular fluid (FF) and to correlate the levels of these substances with oocyte maturation. FF were aspirated from women undergoing in vitro fertilization and embryo transfer. Sera were collected from women with normal menstrual cycles. VEGF in FFs and sera were measured by enzyme linked immunosorbent assay. VEGF, HGF, and angiogenin mRNA expression aspirated folliculars cell was analyzed by reverse transcription and polymerase chain reaction (RT-PCR). The concentrations of VEGF, HGF, and angiogenin in FF were significantly higher than those in serum (P<0.001). VEGF, HGF, and angiogenin mRNA in the aspirated follicles cell was detected by RT-PCR. HGF levels were higher in FF containing mature oocyte. The levels of VEGF in FF containing mature oocytes in women under 39 years of age were significantly lower than those in FF from women more than 40 years old (P<0.01). Our data suggest that VEGF, HGF, and angiogenin may play an important role in follicular growth and development, that VEGF levels in FF appear to be age-dependent; and that VEGF and HGF levels might be valuable biochemical markers of oocyte maturation.
authors.affiliation: Department of Obstetrics and Gynecology, Oita Medical University, Oita 879 5593, Japan. yasushi@oita2.hosp.go.jp
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Kawano
authors.forename: Kosay
authors.initials: K
authors.lastname: Zeineh Hasan
authors.forename: Junichiro
authors.initials: J
authors.lastname: Fukuda
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Mine
authors.forename: Isao
authors.initials: I
authors.lastname: Miyakawa
mesh_terms: D000328:Adult; D002454:Cell Differentiation; D005260:Female; D015571:Follicular Fluid / Q000378:metabolism*; D017228:Hepatocyte Growth Factor / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D066298:In Vitro Techniques; D018919:Neovascularization, Physiologic; D009865:Oocytes / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D009866:Oogenesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012259:Ribonuclease, Pancreatic / Q000096:biosynthesis* / Q000235:genetics; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D017228:Hepatocyte Growth Factor; C046366:angiogenin; D012259:Ribonuclease, Pancreatic