title: Methylation of histone H3 by Set2 in Saccharomyces cerevisiae is linked to transcriptional elongation by RNA polymerase II.
abstract: Set2 methylates Lys36 of histone H3. We show here that yeast Set2 copurifies with RNA polymerase II (RNAPII). Chromatin immunoprecipitation analyses demonstrated that Set2 and histone H3 Lys36 methylation are associated with the coding regions of several genes that were tested and correlate with active transcription. Both depend, as well, on the Paf1 elongation factor complex. The C terminus of Set2, which contains a WW domain, is also required for effective Lys36 methylation. Deletion of CTK1, encoding an RNAPII CTD kinase, prevents Lys36 methylation and Set2 recruitment, suggesting that methylation may be triggered by contact of the WW domain or C terminus of Set2 with Ser2-phosphorylated CTD. A set2 deletion results in slight sensitivity to 6-azauracil and much less beta-galactosidase produced by a reporter plasmid, resulting from a defect in transcription. In synthetic genetic array (SGA) analysis, synthetic growth defects were obtained when a set2 deletion was combined with deletions of all five components of the Paf1 complex, the chromodomain elongation factor Chd1, the putative elongation factor Soh1, the Bre1 or Lge1 components of the histone H2B ubiquitination complex, or the histone H2A variant Htz1. SET2 also interacts genetically with components of the Set1 and Set3 complexes, suggesting that Set1, Set2, and Set3 similarly affect transcription by RNAPII.
authors.affiliation: Banting and Best Department of Medical Research, Toronto Yeast Proteomics Organization, University of Toronto, Toronto, Ontario, Canada M5G 1L6.
authors.forename: Nevan J
authors.initials: NJ
authors.lastname: Krogan
authors.forename: Minkyu
authors.initials: M
authors.lastname: Kim
authors.forename: Amy
authors.initials: A
authors.lastname: Tong
authors.forename: Ashkan
authors.initials: A
authors.lastname: Golshani
authors.forename: Gerard
authors.initials: G
authors.lastname: Cagney
authors.forename: Veronica
authors.initials: V
authors.lastname: Canadien
authors.forename: Dawn P
authors.initials: DP
authors.lastname: Richards
authors.forename: Bryan K
authors.initials: BK
authors.lastname: Beattie
authors.forename: Andrew
authors.initials: A
authors.lastname: Emili
authors.forename: Charles
authors.initials: C
authors.lastname: Boone
authors.forename: Ali
authors.initials: A
authors.lastname: Shilatifard
authors.forename: Stephen
authors.initials: S
authors.lastname: Buratowski
authors.forename: Jack
authors.initials: J
authors.lastname: Greenblatt
mesh_terms: D002843:Chromatin / Q000378:metabolism; D017353:Gene Deletion; D006657:Histones / Q000378:metabolism*; D007074:Immunoglobulin G / Q000378:metabolism; D008239:Lysine / Q000737:chemistry; D008745:Methylation; D008780:Methyltransferases / Q000378:metabolism*; D008954:Models, Biological; D008957:Models, Genetic; D011233:Precipitin Tests; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012319:RNA Polymerase II / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D014158:Transcription, Genetic; D014498:Uracil / Q000031:analogs & derivatives* / Q000494:pharmacology; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D002843:Chromatin; D006657:Histones; D007074:Immunoglobulin G; D029701:Saccharomyces cerevisiae Proteins; D014498:Uracil; D008780:Methyltransferases; C448996:Set2 protein, S cerevisiae; D012319:RNA Polymerase II; D001616:beta-Galactosidase; C100248:azauracil; D008239:Lysine