Proteomics: advanced technology for the analysis of cellular function.
Proteomics developed initially from the decade-long study of comprehensive protein visualization on two-dimensional electrophoresis gels has been expanded by mass spectrometry and the growth in searchable sequence databases. Currently, by use of more sophisticated technology such as a combination of multidimensional chromatography and mass spectrometry, thousands of proteins can automatically be identified in a day along with semiquantitative information on differential-protein expression. As with differential gene expression by cDNA-chips, the differential-protein analysis is useful for monitoring and identifying proteins involved in various physiological changes in cells or organisms, although the analysis alone does not necessarily provide information regarding the cause of the change or the function of the proteins. However, proteomics also provides the tools to expand into more sophisticated biochemical approaches, such as the study of protein interactions that can be determined directly by performing a pull-down assay with a bait protein followed by mass spectrometric identification of the bound proteins. Proteomics, thus, is useful for both large-scale surveys of proteins and detailed studies of the functional relationships among the proteins of interest. Certainly this approach can be applicable to the assessment of amino acid adequacy and safety.
Takahashi
Nobuhiro
N
Department of Applied Biological Science, Faculty of Agriculture, United Graduate School of Agriculture, Tokyo University of Agriculture & Technology, Fuchu-shi, Tokyo 183-8509, Japan. ntakahas@cc.tuat.ac.jp
Kaji
Hiroyuki
H
Yanagida
Mitsuaki
M
Hayano
Toshiya
T
Isobe
Toshiaki
T
D000818:Animals; D002468:Cell Physiological Phenomena; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D013058:Mass Spectrometry; D040081:Protein Array Analysis; D040901:Proteomics