title: Influence of peracetic acid on adhesion/invasion of Salmonella enterica serotype typhimurium LT2.
abstract: The influence of peracetic acid (PAA) disinfectant on Salmonella enterica serotype Typhimurium LT2 in sewage effluent was examined by studying its ability to adhere to and invade HeLa cells in vitro. Although the disinfectant produced a decrease of about 5 log units, the bacteria kept their adhesive and invasive abilities. Scanning microscopic observations of the PAA-treated bacteria revealed that PAA caused a loss of external microfilaments and an alteration of membrane structure. Nevertheless, electron-microscopic observations showed that PAA-treated bacteria were still able to adhere to and invade HeLa cells despite the fact that the bacteria seemed to have undergone some structural modifications. With confocal microscopy, the use of anti-actin antibody showed that the contact between the bacteria (with or without PAA treatment) and the HeLa cells activated actinopolymerization of the HeLa cell cytoskeleton.
authors.affiliation: Equipe de Microbiologie, UPRES 1254, Faculté des Sciences Pharmaceutiques et Biologiques, Université de Rennes I, Rennes, France. anne.gougeon@univ-rennes1.fr
authors.forename: A
authors.initials: A
authors.lastname: Jolivet-Gougeon
authors.forename: F
authors.initials: F
authors.lastname: Sauvager
authors.forename: M
authors.initials: M
authors.lastname: Arturo-Schaan
authors.forename: M
authors.initials: M
authors.lastname: Bonnaure-Mallet
authors.forename: M
authors.initials: M
authors.lastname: Cormier
mesh_terms: D001422:Bacterial Adhesion / Q000187:drug effects*; D002462:Cell Membrane / Q000382:microbiology / Q000648:ultrastructure; D004202:Disinfectants / Q000633:toxicity*; D006367:HeLa Cells; D006801:Humans; D042541:Intracellular Space / Q000382:microbiology / Q000648:ultrastructure; D010463:Peracetic Acid / Q000633:toxicity*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000472:pathogenicity* / Q000648:ultrastructure; D012722:Sewage
chemical_list: D004202:Disinfectants; D012722:Sewage; D010463:Peracetic Acid