[High-dose glucose induces human retinal endothelial cell apoptosis].
OBJECTIVE
To examine the direct effect of high glucose levels on primary cultured human retinal capillary endothelial cells (HRCEC).


METHODS
HRCECs were isolated from donated eyes and cultured for 6 days in the media containing 5 or 25 mmol/L glucose. The cell viability was determined by trypan blue exclusion assay and cell cycle analyzed by flow cytometry, with the cell apoptosis assayed by TUNEL method.


RESULTS
The cell viability was significantly decreased after exposure to 25 mmol/L glucose, and the number of apoptotic cells determined by flow cytometry and TUNEL was significantly increased in response to high-dose glucose treatment.


CONCLUSIONS
High-dose glucose induces apoptosis in HRCEC, which may contribute to the development of diabetic retinopathy.
Ma
Jian-fang
JF
Department of Internal Medicine, 476 Army Hospital, Fuzhou, 350002, China.
Yang
Zhong-han
ZH
Song
Zhi-hong
ZH
Guo
Yin
Y
Cai
Wei-bin
WB
Liu
Qian-ping
QP
Guo
Lin-lang
LL
Gao
Guo-quan
GQ
D017209:Apoptosis / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003930:Diabetic Retinopathy / Q000209:etiology*; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000473:pathology; D005947:Glucose / Q000633:toxicity*; D006801:Humans; D012160:Retina / Q000187:drug effects* / Q000473:pathology
D005947:Glucose