title: [HPV detection and FHIT expression in esophageal squamous carcinoma from high incidence area in Cixian County].
abstract: OBJECTIVE
  Cixian County is one of the high incidence areas of esophageal carcinoma in China. Up to now, no work has been conducted on the possible etiological role of human papilloma virus (HPV) infection on esophageal carcinoma in this local area. The aim of this study was to explore the putative roles of HPV infection on the esophageal carcinogenesis of the patients in Cixian and to elucidate the possible relationship between HPV existence and fragile histidine triad gene (FHIT gene, a putative tumor suppressor gene), expression in squamous cell carcinoma of esophagus.
  
  
  METHODS
  The existence of HPV DNA and the expression of FHIT gene at protein level in esophageal carcinoma tissues were determined with PCR and immunohistochemical staining, respectively, in 128 archival paraffin-embedded tissue blocks of esophageal squamous carcinoma from the high incidence area of Cixian and 24 tissue blocks from the non-high incidence area.
  
  
  RESULTS
  PCR results showed that the positive detection rate of HPV in esophageal carcinoma tissues from the high incidence area was 20.3%, which was slightly higher than that from the non-high incidence area (8.3%)(P >0.05). Immunohistochemically, aberrant expression of FHIT gene in esophageal carcinoma tissue was found in 75.6% cases from the high incidence area and only 54.2% from the non-high incidence area, which was significantly lower than that of the former (P< 0.05). No correlation was found between the abnormal expression of FHIT and existence of HPV DNA in esophageal squamous carcinoma tissues.
  
  
  CONCLUSIONS
  HPV DNA could be detected in partial esophageal carcinoma cases from the high incidence area of esophageal carcinoma in Cixian. The aberrant expression rate of FHIT protein in the cases from the high incidence area of esophageal carcinoma in Cixian is higher than that from the non-high incidence area.
authors.affiliation: Laboratory of Pathology, Hebei Medical University, Shijiazhuang, Hebei, PR China.
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: Liu
authors.forename: Xue-Min
authors.initials: XM
authors.lastname: Li
authors.forename: Guo-Liang
authors.initials: GL
authors.lastname: Jin
authors.forename: Xia
authors.initials: X
authors.lastname: Yan
authors.forename: Jian-Zhu
authors.initials: JZ
authors.lastname: Yang
authors.forename: Jun-Ling
authors.initials: JL
authors.lastname: Wang
authors.forename: Yue-Hong
authors.initials: YH
authors.lastname: Li
authors.forename: Feng-Rong
authors.initials: FR
authors.lastname: Wang
authors.forename: Xiang-Hong
authors.initials: XH
authors.lastname: Zhang
mesh_terms: D017766:Acid Anhydride Hydrolases; D002294:Carcinoma, Squamous Cell / Q000453:epidemiology / Q000378:metabolism / Q000821:virology*; D002681:China / Q000453:epidemiology; D004279:DNA, Viral / Q000032:analysis*; D004938:Esophageal Neoplasms / Q000453:epidemiology / Q000378:metabolism / Q000821:virology*; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000150:complications / Q000453:epidemiology*; D016133:Polymerase Chain Reaction; D014412:Tumor Virus Infections / Q000150:complications / Q000453:epidemiology; D000099170:Bis(5'-adenosyl)triphosphatase
chemical_list: D017766:Acid Anhydride Hydrolases; D004279:DNA, Viral; D009363:Neoplasm Proteins; D000099170:Bis(5'-adenosyl)triphosphatase