Changes in the proton T2 relaxation times of cerebral water and metabolites during forebrain ischemia in rat at 9.4 T.
Proton T(2) relaxation times of cerebral water and metabolites were measured before, during, and after transient forebrain ischemia in rat at 9.4 T using localized proton magnetic resonance spectroscopy ((1)H-MRS) with Hahn echoes formed at different echo times (TEs). It was found that the T(2) values of water and N-acetyl aspartate (NAA) methyl, but not total creatine (tCr) methyl, decrease significantly (approximately 10%) during ischemia, and this T(2) reduction is reversed by reperfusion. The T(2) reduction observed for NAA was most likely caused by the extravascular component of the blood oxygenation level-dependent (BOLD) effect induced by a drastically increased deoxyhemoglobin content during ischemia. The absence of T(2) changes for tCr can probably be explained by the fact that the BOLD-related T(2) decrease was counterbalanced by the conversion of phosphocreatine (PCr) to creatine (Cr), which has a longer T(2) than PCr, during ischemia. The changes in T(2) should be taken into account for the quantification of metabolite concentrations during ischemia.
Lei
Hao
H
Center for Magnetic Resonance Research, Department of Radiology, University of Minnesota Medical School, Minneapolis 55455, USA.
Zhang
Yi
Y
Zhu
Xiao-Hong
XH
Chen
Wei
W
D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives* / Q000378:metabolism*; D002545:Brain Ischemia / Q000378:metabolism*; D003401:Creatine / Q000378:metabolism*; D016014:Linear Models; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D016548:Prosencephalon / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014867:Water / Q000378:metabolism
D014867:Water; D001224:Aspartic Acid; C000179:N-acetylaspartate; D003401:Creatine