IGF-1 signaling enhances cell survival in periodontal ligament fibroblasts vs. gingival fibroblasts.
The role of insulin-like growth factors (IGFs) in the regulation of apoptosis has been suggested, yet their impact on specific cells such as periodontal ligament fibroblasts (PDLF) and gingival fibroblasts (GF) remains unknown. The purpose of this study was to test the role of IGF-1 signaling in cell survival in PDLF compared with GF. In periodontal tissue sections, a significantly reduced apoptotic rate was first demonstrated in PDLF compared with GF. In vitro, IGF-1 substantially enhanced cell survival in PDLF compared with GF by the up-regulation of anti-apoptotic molecules and the down-regulation of pro-apoptotic molecules. Furthermore, the differential expression of insulin-like growth factor binding protein 5 (IGFBP-5) was observed in vitro, and its differential distribution was confirmed in vivo. Analysis of the present data suggests an enhanced cell survival in PDLF compared with GF by the up-regulation of IGF-1 signaling pathway.
Han
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Department of Periodontology & Oral Biology, Goldman School of Dental Medicine, Boston University, 700 Albany Street, W-201E, Boston, MA, 02118, USA.
Amar
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S
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002352:Carrier Proteins / Q000032:analysis; D053148:Caspase 3; D020169:Caspases / Q000032:analysis; D018929:Cell Culture Techniques; D002470:Cell Survival / Q000187:drug effects; D004792:Enzyme Precursors / Q000032:analysis; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D005881:Gingiva / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D018975:Insulin-Like Growth Factor Binding Protein 5 / Q000032:analysis; D007334:Insulin-Like Growth Factor I / Q000032:analysis / Q000494:pharmacology*; D008253:Macaca mulatta; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects*; D017346:Protein Serine-Threonine Kinases; D011505:Protein-Tyrosine Kinases / Q000032:analysis; D011518:Proto-Oncogene Proteins / Q000032:analysis; D051057:Proto-Oncogene Proteins c-akt; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D015398:Signal Transduction / Q000187:drug effects*; D015854:Up-Regulation; D051025:bcl-Associated Death Protein
C494819:BAD protein, human; D002352:Carrier Proteins; D004792:Enzyme Precursors; D018975:Insulin-Like Growth Factor Binding Protein 5; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051025:bcl-Associated Death Protein; D007334:Insulin-Like Growth Factor I; D011505:Protein-Tyrosine Kinases; D017346:Protein Serine-Threonine Kinases; D051057:Proto-Oncogene Proteins c-akt; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases