title: Protein transduction domains: are they delivering?
abstract: Early studies with protein transduction domains (PTDs), such as those derived from Tat and Drosophila Antennapedia, showed rapid, receptor- and endosomal-independent uptake of conjugated biological tools into all cell types. However, recent mechanistic studies suggest that these observations were artefacts of the positively charged nature of PTDs and that uptake is instead via electrostatic binding to the plasma membrane and subsequent endocytosis. Given these observations, we assess the future utility of PTDs for in vitro and in vivo cellular delivery.
authors.affiliation: EST-Biology, AstraZeneca Pharmaceuticals, Mereside, Alderley Park, Cheshire SK10 4TG, UK.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Green
authors.forename: Richard
authors.initials: R
authors.lastname: Christison
authors.forename: Catherine J
authors.initials: CJ
authors.lastname: Voyce
authors.forename: Ken R
authors.initials: KR
authors.lastname: Bundell
authors.forename: Mark A
authors.initials: MA
authors.lastname: Lindsay
mesh_terms: D000818:Animals; D016503:Drug Delivery Systems / Q000379:methods*; D005822:Genetic Vectors; D006801:Humans; D011506:Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D014161:Transduction, Genetic
chemical_list: D011506:Proteins