title: Channel-tunnels: outer membrane components of type I secretion systems and multidrug efflux pumps of Gram-negative bacteria.
abstract: For translocation across the cell envelope of Gram-negative bacteria, substances have to overcome two permeability barriers, the inner and outer membrane. Channel-tunnels are outer membrane proteins, which are central to two distinct export systems: the type I secretion system exporting proteins such as toxins or proteases, and efflux pumps discharging antibiotics, dyes, or heavy metals and thus mediating drug resistance. Protein secretion is driven by an inner membrane ATP-binding cassette (ABC) transporter while drug efflux occurs via an inner membrane proton antiporter. Both inner membrane transporters are associated with a periplasmic accessory protein that recruits an outer membrane channel-tunnel to form a functional export complex. Prototypes of these export systems are the hemolysin secretion system and the AcrAB/TolC drug efflux pump of Escherichia coli, which both employ TolC as an outer membrane component. Its remarkable conduit-like structure, protruding 100 A into the periplasmic space, reveals how both systems are capable of transporting substrates across both membranes directly from the cytosol into the external environment. Proteins of the channel-tunnel family are widespread within Gram-negative bacteria. Their involvement in drug resistance and in secretion of pathogenic factors makes them an interesting system for further studies. Understanding the mechanism of the different export apparatus could help to develop new drugs, which block the efflux pumps or the secretion system.
authors.affiliation: Department of Biotechnology, University of Würzburg, 97074 Würzburg, Germany. andersen@biozentrum.uni-wuerzburg.de
authors.forename: C
authors.initials: C
authors.lastname: Andersen
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000502:physiology*; D001692:Biological Transport; D018360:Crystallography, X-Ray; D024881:Drug Resistance, Bacterial / Q000502:physiology*; D029968:Escherichia coli Proteins; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000502:physiology; D007473:Ion Channels / Q000378:metabolism* / Q000502:physiology; D026901:Membrane Transport Proteins; D008958:Models, Molecular; D004364:Pharmaceutical Preparations / Q000378:metabolism; D011487:Protein Conformation
chemical_list: D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; D007473:Ion Channels; D026901:Membrane Transport Proteins; D004364:Pharmaceutical Preparations; C073126:tolC protein, E coli