Expression of Ruminococcus albus xylanase gene ( xynA) in Streptococcus bovis 12-U-1.
The objective of this study was to ligate the xylanase gene A ( xynA) isolated from Ruminococcus albus 7 into the promoter and signal-peptide region of the lichenase [beta-(1,3-1,4)-glucanase] gene of Streptococcus bovis JB1. This fusion gene was inserted into the pSBE11 vector, and the resulting recombinant, plasmid pXA, was used to transform S. bovis 12-U-1 cells. The transformant, S. bovis 12UXA, secreted the xylanase, which was stable against freeze-thaw treatment and long-time incubation at 37 degrees C. The introduction of pXA and production of xylanase did not affect cell growth, and the xylanase produced degraded xylan from oat-spelt and birchwood.
Nakamura
Mutsumi
M
Rumen Microbiology Research Team, STAFF-Institute, 446-1 Ippaizuka, Kamiyokoba, Tsukuba, Ibaraki 305-0854, Japan.
Nagamine
Takafumi
T
Harada
Chisato
C
Tajima
Kiyoshi
K
Matsui
Hiroki
H
Benno
Yoshimi
Y
D001673:Biodegradation, Environmental; D015870:Gene Expression; D006026:Glycoside Hydrolases / Q000235:genetics; D006095:Gram-Positive Cocci / Q000201:enzymology / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000378:metabolism; D016985:Streptococcus bovis / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D014169:Transformation, Bacterial; D043325:Xylan Endo-1,3-beta-Xylosidase; D014990:Xylans / Q000378:metabolism; D014995:Xylosidases / Q000235:genetics* / Q000378:metabolism
D011994:Recombinant Proteins; D014990:Xylans; D006026:Glycoside Hydrolases; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase; C028020:licheninase