The interaction of metal ions and Marimastat with matrix metalloproteinase 9.
The effect of a range of metal ions on the ability of Marimastat to inhibit matrix metalloproteinase 9 (MMP-9) was examined in a fluorescence based proteolytic assay. Whilst none of the metals examined significantly affected the inhibitory ability of Marimastat, several metal ions did have a significant effect on MMP-9 activity itself. In the absence of Marimastat, Zn(II) and Fe(II) significantly inhibited MMP-9 activity at metal ion concentrations of 10 and 100 microM, respectively. In both the absence and presence of Marimastat, Cd(II) significantly inhibited MMP-9 at 100 microM. In contrast, 1 mM Co(II) significantly upregulated MMP-9 proteolytic activity.
Underwood
C K
CK
Centre for Heavy Metals Research, School of Chemistry, University of Sydney NSW, 2006 Australia.
Min
D
D
Lyons
J G
JG
Hambley
T W
TW
D004347:Drug Interactions; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D006877:Hydroxamic Acids / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D007951:Leukemia, Myeloid; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D061965:Matrix Metalloproteinase Inhibitors; D019216:Metals, Heavy / Q000737:chemistry* / Q000494:pharmacology*; D009000:Monocytes / Q000166:cytology; D013050:Spectrometry, Fluorescence / Q000379:methods; D014357:Trypsin / Q000378:metabolism; D014407:Tumor Cells, Cultured
D004791:Enzyme Inhibitors; D006877:Hydroxamic Acids; D061965:Matrix Metalloproteinase Inhibitors; D019216:Metals, Heavy; C100342:marimastat; D014357:Trypsin; D020780:Matrix Metalloproteinase 9