[A novel approach to quality evaluation of root of Scutellaria baicalensis by DPPH free radical scavenging].
OBJECTIVE
To develop a simple, reliable approach for evaluating the quality of Huangqin (Scutellaria baicalensis).


METHODS
To determine the DPPH free radical scavenging activity and assay of four bioactive components: baicalin, baicalein, wogonin and wogonin-7-O-glucuronide by HPLC.


RESULTS
The correlative relationship between DPPH free radical scavenging activity and baicalin content was obtained.


CONCLUSIONS
Bioassay of DPPH free radical scavenging activity could be used as one of the methods for quality evaluation of Chinese drug Huangqin.
Liu
Yu-ping
YP
Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
Basnet
Purusotam
P
Komatsu
Katsuko
K
Cao
Hui
H
D001713:Biphenyl Compounds; D044950:Flavanones; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology; D005609:Free Radicals; D015394:Molecular Structure; D010851:Picrates; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D031364:Scutellaria baicalensis / Q000737:chemistry*
D001713:Biphenyl Compounds; D044950:Flavanones; D005419:Flavonoids; D016166:Free Radical Scavengers; D005609:Free Radicals; D010851:Picrates; C038044:baicalin; C006680:baicalein; C004931:1,1-diphenyl-2-picrylhydrazyl