title: T cells are required for host protection against Brugia malayi but need not produce or respond to interleukin-4.
abstract: T cells are known to be required for host protection in mouse models of Brugia malayi infection. Several independent studies in murine models have also highlighted the rapid induction of Th2-like responses after infection with B. malayi or B. pahangi. Previous data from our laboratory have described a significant increase in permissiveness in the absence of interleukin-4 (IL-4), the "prototypical" Th2 cytokine, involved in both the induction and maintenance of Th2 responses. These observations led to our hypothesis that T cells involved in murine host protection would respond to IL-4 signaling and differentiate into cells of the "type 2" phenotype. As such, these cells would presumably also act as major sources of IL-4. To investigate these hypotheses, we performed several adoptive transfers in which we controlled the cell population(s) able to produce or respond to IL-4. We show here that, in contrast to our original hypotheses, IL-4 production and IL-4 receptor expression by T cells are both dispensable for T-cell-mediated host protection. Instead, our data imply that T cells may be required for eosinophil accumulation at the site of infection.
authors.affiliation: Department of Pathology, University of Connecticut Health Center, Farmington 06030, USA.
authors.forename: L
authors.initials: L
authors.lastname: Spencer
authors.forename: L
authors.initials: L
authors.lastname: Shultz
authors.forename: T V
authors.initials: TV
authors.lastname: Rajan
mesh_terms: D000818:Animals; D017178:Brugia malayi; D004804:Eosinophils / Q000502:physiology; D005368:Filariasis / Q000276:immunology*; D018793:Interleukin-13 / Q000502:physiology; D015847:Interleukin-4 / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID; D019948:Receptors, Interleukin-4 / Q000502:physiology; D050800:STAT6 Transcription Factor; D013601:T-Lymphocytes / Q000276:immunology*; D015534:Trans-Activators / Q000502:physiology
chemical_list: D018793:Interleukin-13; D019948:Receptors, Interleukin-4; D050800:STAT6 Transcription Factor; C494100:Stat6 protein, mouse; D015534:Trans-Activators; D015847:Interleukin-4