title: Protective role of cyclosporine in experimental unilateral blunt testicular trauma: evaluation by 31P MR spectroscopy.
abstract: Magnetic resonance (MR) imaging is a useful tool to study the anatomy of the testis while 31P magnetic resonance spectroscopy (MRS) provides a non-invasive alternative method to demonstrate the metabolic status of testes. This study was designed to test whether the protective role of cyclosporine in experimental unilateral blunt testicular trauma (UBTT) could be assessed by 31P MRS. Male Wistar rats (n = 30) aged 20 days were randomised into group I (sham surgery), group II (UBTT) and group III (UBTT and cyclosporine for 7 days). Contralateral testicles of 5 rats from each group was evaluated by 31P MRS at 30 and 60 days of age and phosphomonoesters (PM), phosphodiesters (PD), and adenosine triphosphate (ATP) levels were measured. At 60 days of age the PM/ATP ratio was 0.32+/-0.08 in group I whereas it was 0.68+/-0.31 in the group II (p<0.05). Group III rats showed PM, PD and PM/ATP ratios similar to the controls. In conclusion, it is observed that UBTT causes contralateral testicular damage which could be prevented by short-term cyclosporine treatment and 31P MRS is an excellent modality for such an evaluation.
authors.affiliation: Department of Paediatric Surgery, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: M
authors.initials: M
authors.lastname: Srinivas
authors.forename: Mahaveer
authors.initials: M
authors.lastname: Degaonkar
authors.forename: N R
authors.initials: NR
authors.lastname: Jagannathan
authors.forename: M M
authors.initials: MM
authors.lastname: Misro
authors.forename: S P
authors.initials: SP
authors.lastname: Chaki
authors.forename: D K
authors.initials: DK
authors.lastname: Gupta
mesh_terms: D000818:Animals; D015551:Autoimmunity / Q000187:drug effects*; D016572:Cyclosporine / Q000627:therapeutic use*; D007111:Immunity, Cellular / Q000187:drug effects*; D007166:Immunosuppressive Agents / Q000627:therapeutic use*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013737:Testis / Q000293:injuries*; D014949:Wounds, Nonpenetrating / Q000145:classification*
chemical_list: D007166:Immunosuppressive Agents; D016572:Cyclosporine