title: The effect of immunonutrition on bacterial translocation, and intestinal villus atrophy in experimental obstructive jaundice.
abstract: OBJECTIVE
  Spontaneous bacterial infection and septicemia due to increased bacterial translocation (BT) in patients with obstructive jaundice result in significant morbidity and mortality. The present study evaluates the effects of enteral nutrition with immune enhancing feeds on BT and intestinal villus histopathology promoted by obstructive jaundice.
  
  
  METHODS
  Fifty male Wistar-albino rats weighing 250-300g were assigned into five equal groups of 10. Animals in Groups I, II, and III were fed with standard chow, those in Group IV were given glutamine 1g/kg/day and the remaining 10 animals in Group V were fed with an arginine, omega-3 fatty acids, and RNA-supplemented enteral diet for (1g/kg/day amino acid and 230 kcal/kg) 7 days preoperatively. Group I underwent sham operation and the remaining animals in all other groups underwent common bile duct ligation. After operation, Group I had standard chow, Groups II and IV had glutamine, Groups III and V had an arginine omega-3 fatty acids, and RNA-supplemented enteral diet for 7 days. All animals were sacrificed on the 8th postoperative day and evaluated both biochemically and histopathologically. Samples from blood, liver, mesenteric lymph nodes and spleen were cultured under aerobic conditions.
  
  
  RESULTS
  Significantly less BT was observed in groups fed with an arginine, omega-3 fatty acids, and RNA-supplemented enteral diet or glutamine in pre-and postoperative periods as compared to others (P<0.001). Histologic evaluation also showed significant reduction in villus atrophy in these groups.
  
  
  CONCLUSIONS
  Enteral immunonutrition using glutamine or arginine, omega-3 fatty acids, and RNA-supplemented enteral diet during both pre-and postoperative periods seems to reduce BT and decrease atrophy of intestinal mucosal villi in rats with obstructive jaundice.
authors.affiliation: Department of Surgery, Ankara Numune Teaching and Research Hospital, 06100 Ankara, Turkey.
authors.forename: B
authors.initials: B
authors.lastname: Zulfikaroglu
authors.forename: E
authors.initials: E
authors.lastname: Zulfikaroglu
authors.forename: M M
authors.initials: MM
authors.lastname: Ozmen
authors.forename: N
authors.initials: N
authors.lastname: Ozalp
authors.forename: R
authors.initials: R
authors.lastname: Berkem
authors.forename: S
authors.initials: S
authors.lastname: Erdogan
authors.forename: H T
authors.initials: HT
authors.lastname: Besler
authors.forename: M
authors.initials: M
authors.lastname: Koc
authors.forename: A
authors.initials: A
authors.lastname: Korkmaz
mesh_terms: D000818:Animals; D001120:Arginine / Q000008:administration & dosage; D001284:Atrophy; D018988:Bacterial Translocation / Q000187:drug effects*; D001651:Cholestasis, Extrahepatic / Q000382:microbiology* / Q000628:therapy; D004195:Disease Models, Animal; D004750:Enteral Nutrition; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage; D005973:Glutamine / Q000008:administration & dosage; D007413:Intestinal Mucosa / Q000187:drug effects / Q000382:microbiology / Q000473:pathology*; D008099:Liver / Q000382:microbiology; D008198:Lymph Nodes / Q000382:microbiology; D008297:Male; D008643:Mesentery; D009747:Nutritional Physiological Phenomena; D012313:RNA / Q000008:administration & dosage; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000382:microbiology
chemical_list: D015525:Fatty Acids, Omega-3; D005973:Glutamine; D012313:RNA; D001120:Arginine