title: [Mechanism of overproduction of secreted enzymes in the mycelial fungus Penicillium canescens].
abstract: The fungus Penicillium canescens strain F178 (VKPM) and its niaD- mutant exhibited an increased capability of synthesizing extracellular enzymes beta-galactosidase (70-80 U/ml) and xylanase (100 U/ml). The synthesis was induced by arabinose and its catabolite, arabitol. A deficiency in arabitol dehydrogenase, leading to arabitol accumulation in the cell, was detected in the chain of reactions of arabinose catabolism. The increased synthesis of beta-galactosidase and xylanase in P. canescens is accounted for by (1) cellular accumulation of the inducer (arabitol) at low concentrations of arabinose in the medium and (2) prevalence of induction over repression.
authors.affiliation: All-Russian Research Institute of Genetics and Selection of Industrial Microorganisms, Pervyi Dorozhnyi Proezed 1, Moscow, 113545 Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Vavilova
authors.forename: S V
authors.initials: SV
authors.lastname: Antonova
authors.forename: E D
authors.initials: ED
authors.lastname: Barsukov
authors.forename: Iu P
authors.initials: IuP
authors.lastname: Vinetskiĭ
mesh_terms: D000426:Alcohol Dehydrogenase / Q000172:deficiency; D001089:Arabinose / Q000378:metabolism; D003470:Culture Media; D009154:Mutation; D025282:Mycelium / Q000201:enzymology; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000172:deficiency / Q000235:genetics; D010407:Penicillium / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D013402:Sugar Alcohols / Q000378:metabolism; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000096:biosynthesis*; D001616:beta-Galactosidase / Q000096:biosynthesis*
chemical_list: D003470:Culture Media; D013402:Sugar Alcohols; D001089:Arabinose; D000426:Alcohol Dehydrogenase; D009565:Nitrate Reductases; D050901:Nitrate Reductase; D014995:Xylosidases; D001616:beta-Galactosidase; D043325:Xylan Endo-1,3-beta-Xylosidase; C014999:arabitol