title: Prevention and suppression of Helicobacter felis infection in mice using colostral preparation with specific antibodies.
abstract: BACKGROUND
  Specific antibodies against Helicobacter were enriched from the colostra of hyperimmunized cows. Efficacies of colostral control preparation and immune preparation containing specific antibodies against Helicobacter felis were studied in the prevention and treatment of experimental H. felis infection in mice.
  
  
  METHODS
  H. felis-infected mice were given either immune or control preparation with or without complement or amoxicillin orally in four different trials. H. felis status was assessed on the basis of bacterial stainings, gastric histology and serum antibodies.
  
  
  RESULTS
  Immune, but not control preparation, prevented H. felis infection (p > 0.01), the efficacy being dependent on the presence of specific antibodies. In the trial on infected Balb/c mice treatment with immune preparation (p = 0.029) but not control preparation decreased the colonization of gastric antrum by H. felis. In the further trials with infected SJL-mice, treatments with colostral preparations did not decrease colonization. Amoxicillin treatment decreased the colonization with trend-setting significance (p = 0.056; infected mice as controls), whereas amoxicillin combined with immune preparation had a significant effect (p < 0.0005).
  
  
  CONCLUSIONS
  Specific colostral antibodies were useful in the prevention of Helicobacter infection in a mouse model. The results of the treatment trials were controversial but a similar colostral immune preparation against H. pylori could be effective and useful in preventing infections in humans and during antibiotic treatment.
authors.affiliation: MTT, Agrifood Research Finland, Food Research, Jokioinen, Finland.
authors.forename: Pertti
authors.initials: P
authors.lastname: Marnila
authors.forename: Susanna
authors.initials: S
authors.lastname: Rokka
authors.forename: Leena
authors.initials: L
authors.lastname: Rehnberg-Laiho
authors.forename: Päivi
authors.initials: P
authors.lastname: Kärkkäinen
authors.forename: Timo U
authors.initials: TU
authors.lastname: Kosunen
authors.forename: Hilpi
authors.initials: H
authors.lastname: Rautelin
authors.forename: Marja-Liisa
authors.initials: ML
authors.lastname: Hänninen
authors.forename: Eeva-Liisa
authors.initials: EL
authors.lastname: Syväoja
authors.forename: Hannu
authors.initials: H
authors.lastname: Korhonen
mesh_terms: D000658:Amoxicillin / Q000494:pharmacology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000494:pharmacology*; D000918:Antibody Specificity; D002417:Cattle; D015169:Colony Count, Microbial; D003126:Colostrum / Q000276:immunology*; D003131:Combined Modality Therapy; D005260:Female; D016481:Helicobacter Infections / Q000188:drug therapy* / Q000276:immunology / Q000517:prevention & control*; D007116:Immunization, Passive / Q000379:methods; D051379:Mice; D010406:Penicillins / Q000494:pharmacology*
chemical_list: D000907:Antibodies, Bacterial; D010406:Penicillins; D000658:Amoxicillin