Broadband RFDR with adiabatic inversion pulses.
With a view to obtain (13)C chemical shift correlation spectra of uniformly labelled peptides/proteins at high magnetic fields and high magic angle spinning frequencies (omega(r)/2pi </= 20 kHz), the efficacy of RFDR with adiabatic inversion pulses has been assessed via numerical simulations and experimental measurements employing different adiabatic pulse phasing schemes, shapes and durations. It is demonstrated that homonuclear dipolar recoupling with superior performance under resonance offset and H(1) inhomogeneity effects and without strong dependence on the (13)C chemical shift differences can be achieved with adiabatic pulses. It is shown that (13)C chemical shift correlation spectra in the entire range of carbon chemical shifts can be obtained efficiently with short adiabatic inversion pulses. In situations where correlation spectra of only the aliphatic region are required, the possibility for minimising the interference between the recoupling and decoupling RF fields with long adiabatic pulses, at low recoupling power levels and without compromising the broadband RFDR characteristics, is also indicated.
Leppert
Jörg
J
Abteilung Molekulare Biophysik/NMR-Spektroskopie, Institut für Molekulare Biotechnologie, 07745 Jena, Germany.
Heise
Bert
B
Ohlenschläger
Oliver
O
Görlach
Matthias
M
Ramachandran
Ramadurai
R
D000595:Amino Acid Sequence; D002247:Carbon Isotopes; D003198:Computer Simulation; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008962:Models, Theoretical; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D011846:Radio Waves
D002247:Carbon Isotopes; D010455:Peptides; D011506:Proteins