[Effects of mtDNA deletion associated with abnormal expression in rat cochlear with presbycusis].
OBJECTIVE
To investigate the effects of the common deletion (4834-bp) of mitochondrial DNA (mtDNA) in rat cochlear with presbycusis.


METHODS
The mtDNA 4834-bp deletion was analyzed by PCR. The mitochondrial-encoded cytochrome c oxidase subunit I (COXI) transcript level and cytochrome c oxidase (COX) activity were measured by RT-PCR and histochemical methods respectively.


RESULTS
The 4834-bp deletion occurred in all the senescent rat cochlear. The COXI transcript level was decreased associated with the decline of COX activity.


CONCLUSIONS
The mtDNA 4834-bp deletion presented in the rat cochlear with presbycusis, which lead to the decrease of COXI transcript level and COX activity, may play an important role in the pathogens of presbycusis.
Wu
Haiyan
H
Department of Otorhinolaryngology, First Hospital, Jilin University, Changchun 130021, China. wuhaiyan72@perfectmail.com
Du
Baodong
B
Wang
Ping
P
Niu
Chunbo
C
Jin
Xin
X
D000818:Animals; D003051:Cochlea / Q000201:enzymology*; D004272:DNA, Mitochondrial / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000378:metabolism; D005260:Female; D008297:Male; D011304:Presbycusis / Q000201:enzymology / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar; D017384:Sequence Deletion
D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV