title: Influence of Pyriproxyfen on the Expression of Haemolymph Protein Genes in the Colorado Potato Beetle, Leptinotarsa decemlineata.
abstract: Pyriproxyfen, a potent juvenile hormone analogue for the Colorado potato beetle, Leptinotarsa decemlineata, was applied topically to last-instar larvae and short-day adults at different times after moulting. The effect of the hormone analogue on concentration and composition of protein in the haemolymph was studied at different intervals after pyriproxyfen application. The hormone analogue had little effect on total protein concentration of the haemolymph, but affected protein composition. Diapause protein 1 was prevented from being synthesized if pyriproxyfen was applied before the gene was activated and disappeared from the haemolymph if applied after the gene had been expressed. It therefore inactivated the gene for diapause protein in both larvae and adults. Pyriproxyfen also induced appearance of vitellogenin at both stages, indicating induction of expression of the vitellogenin gene. It also affected the stability of mRNA for diapause protein. The analogue caused mRNA for diapause protein 1 to disappear untimely compared to controls in last-instar larvae and short-day adults. The response of adults to the JHA was much more pronounced than that of larvae, although the analogue had a strong biological effect on last-instar larvae because it prevented metamorphosis at low doses. Copyright 1997 Elsevier Science Ltd. All rights reserved
authors.affiliation: Dept of Virology, PO Box 8045, 6700 EM, Wageningen, Netherlands
authors.forename: A M.W.
authors.initials: AM
authors.lastname: VERMUNT
authors.forename: A B.
authors.initials: AB
authors.lastname: KOOPMANSCHAP
authors.forename: C A.D.
authors.initials: CA
authors.lastname: de KORT