title: Detection of human papillomavirus 60 in epidermal cysts of nonpalmoplantar location.
abstract: Human papillomavirus (HPV) types 57 and 60 are associated with epidermal cysts of palmoplantar location (PPECs). Recently, there was a report of HPV 60 in a wart located in a nonpalmoplantar area as a possible precursor of a nonpalmoplantar epidermal cyst (NPPEC). There has been no study that has examined the presence and frequency of HPV in ordinary NPPECs. We reviewed 63 cases of epidermal cysts, including 59 NPPECs and 4 PPECs. After routine histopathologic review, polymerase chain reaction (PCR) of extracted DNA, sequencing of PCR products, and, finally, a homology search were undertaken. As a result, 9 of 63 (14.3%) cysts, including 6 of 59 NPPECs (10.2%), showed a positive reaction on PCR. By homology search after PCR, all were demonstrated as HPV 60. For NPPECs, no variables, including some histopathologic features, are significantly related to the presence of HPV 60 in univariate or multivariate analysis (P > 0.05).
authors.affiliation: Department of Dermatology, School of Medicine, Kyungpook National University Hospital, 50, Samduck-2-ga, Chung-gu, Daegu, South Korea. seokjong@knu.ac.kr
authors.forename: Seokjong
authors.initials: S
authors.lastname: Lee
authors.forename: Wonchae
authors.initials: W
authors.lastname: Lee
authors.forename: Sanglip
authors.initials: S
authors.lastname: Chung
authors.forename: Dowon
authors.initials: D
authors.lastname: Kim
authors.forename: Mihyeung
authors.initials: M
authors.lastname: Sohn
authors.forename: Moonkyu
authors.initials: M
authors.lastname: Kim
authors.forename: Jungchul
authors.initials: J
authors.lastname: Kim
authors.forename: Hanik
authors.initials: H
authors.lastname: Bae
authors.forename: Shin
authors.initials: S
authors.lastname: Kam
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis; D004814:Epidermal Cyst / Q000473:pathology / Q000821:virology*; D005260:Female; D006801:Humans; D007642:Keratosis / Q000473:pathology / Q000821:virology; D008297:Male; D008875:Middle Aged; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012867:Skin / Q000473:pathology*; D012871:Skin Diseases / Q000473:pathology / Q000821:virology*
chemical_list: D017931:DNA Primers; D004279:DNA, Viral