Influences of dibutyryl cyclic adenosine monophosphate and forskolin on human sperm motility in vitro.
OBJECTIVE
To study the influences of dibutyryl cyclic adenosine monophosphate (dbcAMP) and forskolin on human sperm motility in vitro.


METHODS
Semen samples, aseptically obtained by masturbation and prepared by swim-up technique from 20 fertile men, were incubated with different concentrations of dbcAMP and forskolin at 37 deg. Measurements were carried out after 10 min, 20 min, 30 min and 60 min incubation. Motility parameters were estimated by using an automatic analyzing system.


RESULTS
Treatment with dbcAMP or forskolin resulted in a significant increase in sperm motility and progressive motility. The larger the concentrations of dbcAMP or forskolin, the greater the effect appeared. The straight linear velocity and curvilinear velocity were not affected by both agents.


CONCLUSIONS
dbcAMP and forskolin increase the motility and progressive motility of human sperm in vitro.
Liu
Ji-Hong
JH
Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. jhliu888@hotmail.com
Li
Yang
Y
Cao
Zheng-Guo
ZG
Ye
Zhang-Qun
ZQ
D000328:Adult; D003994:Bucladesine / Q000008:administration & dosage / Q000494:pharmacology*; D005576:Colforsin / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D009994:Osmolar Concentration; D013081:Sperm Motility / Q000187:drug effects*
D005576:Colforsin; D003994:Bucladesine