Involvement of capsaicin-sensitive sensory nerves in cardioprotection of rutaecarpine in rats.
In the present study, we examined whether rutaecarpine protects against myocardial ischemia-reperfusion injury in rats and whether the protective effects of rutaecarpine are related to activation of capsaicin-sensitive sensory nerves. Rats were pretreated with rutaecarpine 10 min before the experiment, and then the left main coronary artery of rat hearts was subjected to 60-min occlusion followed by 3-h reperfusion. The infarct size, serum concentration of creatine kinase, and CGRP concentration in plasma were measured. Pretreatment with rutaecarpine (100 or 300 microg/kg, i.v.) significantly reduced infarct size and creatine kinase release concomitantly with a significant increase in plasma concentrations of CGRP. These effects of rutaecarpine were completely abolished by capsazepine (38 mg/kg, s.c.), a competitive vanilloid receptor antagonist, or by pretreatment with capsaicin (50 mg/kg, s.c.), which selectively depletes transmitters in capsaicin-sensitive sensory nerves. These results suggest that rutaecarpine protects against myocardial ischemia-reperfusion injury in rats and that the protective effects of rutaecarpine are related to activation of capsaicin-sensitive sensory nerves via activating vanilloid receptors.
Hu
Chang-Ping
CP
Department of Pharmacology, School of Pharmaceutical Sciences, Central South University, Changsha, Hunan 410078, China.
Li
Nian-Sheng
NS
Xiao
Liang
L
Deng
Han-Wu
HW
Li
Yuan-Jian
YJ
D000470:Alkaloids / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000097:blood; D002211:Capsaicin / Q000031:analogs & derivatives* / Q000633:toxicity*; D002316:Cardiotonic Agents / Q000494:pharmacology*; D003402:Creatine Kinase / Q000097:blood; D006321:Heart / Q000187:drug effects; D026121:Indole Alkaloids; D008297:Male; D009203:Myocardial Infarction / Q000473:pathology; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000517:prevention & control*; D009475:Neurons, Afferent / Q000187:drug effects* / Q000502:physiology*; D009929:Organ Size; D011799:Quinazolines; D051381:Rats; D017208:Rats, Wistar; D052999:Quinazolinones
D000470:Alkaloids; D015740:Calcitonin Gene-Related Peptide; D002211:Capsaicin; D002316:Cardiotonic Agents; D003402:Creatine Kinase; D026121:Indole Alkaloids; D011799:Quinazolines; C028632:rutecarpine; D052999:Quinazolinones; C071423:capsazepine