[Primary culture and biologic characters of human laryngeal papillomavirus positive epithelial cells in children].
OBJECTIVE
To investigate the biologic characters of HPV positive laryngeal epithelial cells in vitro.


METHODS
To draw a cell growth curve by observing the biologic characters of HPV positive cells' growth pattern, PCR and nuclear acid hybridization were applied before and after culture for confirming HPV positive cells DNA.


RESULTS
Primary HPV positive cells often remain viable in culture for more than six weeks, there have HPV DNA before and after culture. We found that in these cells had common characters: lag phase, growth phase and plateau phase. The cells culture showed that the first to forth day there have many cells emigration from tissue pieces. The fifth to Seventh day was latent phase, it was mainly adhere growth in this period, proliferation of culture cells wasn't active. The eighth to eighteenth day was growth phase, cells number was increased quickly, speed of growth was faster. Then cells growth entered to plateau phase, cells number was increased slowly, cells showed vacuoles obviously. It indicated that these cells came to die.


CONCLUSIONS
Cells derives from HPV positive laryngeal epithelial have been successfully cultured, but to establish an animal model for HPV infection is difficult.
Jiang
Xun
X
Department of Otorhinolaryngology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China. Jiangxun@sohu.com
Chen
Wenxian
W
Zhang
Zhipei
Z
Zhang
Dianzhong
D
Cui
Pengcheng
P
Li
Guize
G
Gao
Pengfei
P
Sun
An
A
D002648:Child; D002675:Child, Preschool; D015344:DNA Probes, HPV; D004847:Epithelial Cells / Q000821:virology; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology* / Q000821:virology; D008297:Male; D010212:Papilloma / Q000473:pathology* / Q000821:virology; D027383:Papillomaviridae; D030361:Papillomavirus Infections / Q000473:pathology* / Q000821:virology; D014407:Tumor Cells, Cultured
D015344:DNA Probes, HPV