[Effect of PTH, phosphate, and metabolic acidosis on the progression of renal insufficiency in the azotemic rat].
In a previous study we have observed that NH4Cl-induced metabolic acidosis halted the progression of renal disease in azotemic rats with a high phosphate diet. We hypothesized that NH4Cl-induced metabolic acidosis may exert its protective effect by decreasing renal calcium content independent of serum levels of PTH and phosphate loading. To test this hypothesis we studied azotemic rats with very low phosphate diet or parathyroidectomy. Rats with low phosphate diet and parathyroidectomized rats developed renal failure after 5/6 nephrectomy, and in both groups the acid loading significantly decreased the progression of renal disease. Calcium renal content increased in both groups, even in rats with low phosphate diet, and this effect was also significantly decreased after an acid loading. Rats with acid loading developed greater hypertrophy of renal tissue than rats without acid loading. We conclude that NH4Cl-induced metabolic acidosis halted the progression of renal disease by decreasing calcium precipitation on renal tissue. Parathyroidectomy did not prevent progression of renal disease nor calcium precipitation, and a low phosphate diet in azotemic rats did not prevent increased calcium content on remnant renal tissue.
Jara
A
A
Departamento de Nefrología, Facultad de Medicina, Pontificia Universidad Católica de Chile. ajara@med.puc.cl
Chacón
C
C
Valdivieso
A
A
Ibaceta
M
M
D000138:Acidosis / Q000139:chemically induced / Q000150:complications* / Q000378:metabolism; D000643:Ammonium Chloride / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D002114:Calcinosis / Q000209:etiology / Q000517:prevention & control; D002118:Calcium / Q000032:analysis; D003404:Creatinine / Q000097:blood; D004195:Disease Models, Animal; D018450:Disease Progression; D007668:Kidney / Q000737:chemistry / Q000473:pathology; D007676:Kidney Failure, Chronic / Q000150:complications / Q000378:metabolism*; D008657:Metabolic Clearance Rate / Q000187:drug effects; D009929:Organ Size / Q000187:drug effects; D010281:Parathyroid Hormone / Q000502:physiology*; D016105:Parathyroidectomy; D016226:Phosphorus, Dietary / Q000008:administration & dosage / Q000009:adverse effects*; D051381:Rats; D014511:Uremia / Q000378:metabolism*
D010281:Parathyroid Hormone; D016226:Phosphorus, Dietary; D000643:Ammonium Chloride; D003404:Creatinine; D002118:Calcium