title: Double vital staining using trypan blue and infracyanine green in macular pucker surgery.
abstract: OBJECTIVE
  To study the clinical properties of double vital staining in premacular fibrosis, facilitating complete removal of all epiretinal tissue.
  
  
  METHODS
  In a two step surgery, the epiretinal pucker was removed after staining with trypan blue, whereafter the inner limiting membrane was peeled after staining with infracyanine green.
  
  
  RESULTS
  In all 30 patients, a separate epiretinal layer and inner limiting membrane were removed from the macular area. Pathological examination showed different histological properties of the removed layers. An increased visual acuity was measured in 26 patients, and a slightly decreased visual acuity in one patient.
  
  
  CONCLUSIONS
  The described double staining technique could be a novel valuable tool that may help to achieve optimal anatomical and functional recovery after surgery for premacular fibrosis
authors.affiliation: Department of Ophthalmology, UZLeuven, Belgium. Peter.Stalmans@uz.kuleuven.ac.be
authors.forename: P
authors.initials: P
authors.lastname: Stalmans
authors.forename: E J
authors.initials: EJ
authors.lastname: Feron
authors.forename: R
authors.initials: R
authors.lastname: Parys-Van Ginderdeuren
authors.forename: A
authors.initials: A
authors.lastname: Van Lommel
authors.forename: G R J
authors.initials: GR
authors.lastname: Melles
authors.forename: M
authors.initials: M
authors.lastname: Veckeneer
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D004396:Coloring Agents; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007208:Indocyanine Green / Q000031:analogs & derivatives*; D008266:Macula Lutea / Q000601:surgery*; D008297:Male; D008875:Middle Aged; D012164:Retinal Diseases / Q000175:diagnosis / Q000503:physiopathology / Q000601:surgery*; D013194:Staining and Labeling / Q000379:methods; D014343:Trypan Blue; D014792:Visual Acuity
chemical_list: D004396:Coloring Agents; C116320:Infracyanine green; D014343:Trypan Blue; D007208:Indocyanine Green