Effects of tRNA 3 Lys aminoacylation on the initiation of HIV-1 reverse transcription.
HIV-1 utilizes cellular tRNA(3)(Lys) to prime the initiation of reverse transcription. The selective incorporation of cytoplasmic tRNA(3)(Lys) into HIV-1 particles was recently shown to involve the lysyl-tRNA synthetase, and hence, the encapsidated tRNA(3)(Lys) is likely to be aminoacylated. Here, we tested the effect of aminoacylation on the initiation of reverse transcription. We show that HIV-1 reverse transcriptase is unable to extend lysyl-tRNA(3)(Lys). In addition, the viral polymerase does not significantly enhance the rate of tRNA deacylation, in contrast with previous studies on avian retroviruses. Thus, aminoacylation of the primer tRNA might prevent the initiation of HIV-1 reverse transcription from taking place before viral budding and maturation.
Rigourd
Mickaël
M
Unité Propre de Recherche 9002 du CNRS conventionnée à l'Université Louis-Pasteur, IBMC, 15, rue René-Descartes, 67084 cedex, Strasbourg, France.
Bec
Guillaume
G
Bénas
Philippe
P
Le Grice
Stuart F J
SF
Ehresmann
Bernard
B
Ehresmann
Chantal
C
Marquet
Roland
R
D000123:Acetyltransferases / Q000378:metabolism; D000215:Acylation; D000818:Animals; D002417:Cattle; D054303:HIV Reverse Transcriptase / Q000494:pharmacology; D015497:HIV-1 / Q000502:physiology*; D012313:RNA / Q000235:genetics; D012357:RNA, Transfer, Lys / Q000737:chemistry* / Q000187:drug effects; D014158:Transcription, Genetic / Q000187:drug effects / Q000502:physiology*; D019065:Virus Assembly
C034477:RNA primers; D012357:RNA, Transfer, Lys; D012313:RNA; D000123:Acetyltransferases; D054303:HIV Reverse Transcriptase