SPINE 2: a system for collaborative structural proteomics within a federated database framework.
We present version 2 of the SPINE system for structural proteomics. SPINE is available over the web at http://nesg.org. It serves as the central hub for the Northeast Structural Genomics Consortium, allowing collaborative structural proteomics to be carried out in a distributed fashion. The core of SPINE is a laboratory information management system (LIMS) for key bits of information related to the progress of the consortium in cloning, expressing and purifying proteins and then solving their structures by NMR or X-ray crystallography. Originally, SPINE focused on tracking constructs, but, in its current form, it is able to track target sample tubes and store detailed sample histories. The core database comprises a set of standard relational tables and a data dictionary that form an initial ontology for proteomic properties and provide a framework for large-scale data mining. Moreover, SPINE sits at the center of a federation of interoperable information resources. These can be divided into (i) local resources closely coupled with SPINE that enable it to handle less standardized information (e.g. integrated mailing and publication lists), (ii) other information resources in the NESG consortium that are inter-linked with SPINE (e.g. crystallization LIMS local to particular laboratories) and (iii) international archival resources that SPINE links to and passes on information to (e.g. TargetDB at the PDB).
Goh
Chern-Sing
CS
Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Avenue, New Haven, CT 06520, USA.
Lan
Ning
N
Echols
Nathaniel
N
Douglas
Shawn M
SM
Milburn
Duncan
D
Bertone
Paul
P
Xiao
Rong
R
Ma
Li-Chung
LC
Zheng
Deyou
D
Wunderlich
Zeba
Z
Acton
Tom
T
Montelione
Gaetano T
GT
Gerstein
Mark
M
D003299:Cooperative Behavior; D003628:Database Management Systems; D030562:Databases, Protein; D020407:Internet; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D040901:Proteomics; D012984:Software; D018511:Systems Integration
D011506:Proteins