[Polymorphisms of six short tandem repeat loci in Zhejiang She ethnic population and Zhejiang Hans of China].
OBJECTIVE
To investigate genetic polymorphism of six short tandem repeat (STR) loci (D3S1358,D16S539, TH01,TPOX, CSF1PO,D7S820) in Zhejiang She ethnic population and Han population.


METHODS
By use of AmpFlSTR Cofiler kit, 6 STR loci in 108 She samples and 102 Han samples were amplified. The PCR products were electrophoresed by ABI Prism 377 sequencer; the data were analyzed by Genescan software.


RESULTS
All genotype frequencies of the 6 STR loci in She and Han ethnic groups met Hardy-Weinberg equilibrium. In the She population, the heterozygosities (H) in D3S1358,D16S539,TH01,TPOX, CSF1PO and D7S820 were 0.8028, 0.9148, 0.7522, 0.6728, 0.9123,0.8338, the exclusion of paternity(EP) were 0.4067, 0.6057, 0.4437, 0.3200, 0.5250, 0.5358, discrimination power (DP) were 0.6690, 0.7841, 0.6447, 0.5382, 0.7298, 0.7296.The combined DP, PE and polymorphism information content were 0.9991,0.9805,0.9988 respectively. There were significant differences at D3S1358, D16S539 and TPOX loci, compared with Hans.


CONCLUSIONS
She population has its own STR allele distribution characteristic. The above data obtained from She population can be used not only in genetic researches and population investigation, but also in human identity and paternity testing.
He
Ji
J
Zhejiang Blood Center, Hangzhou, Zhejiang, 310020 PR China. heji1016@sina.com
Xu
Xian-guo
XG
Fu
Qi-hua
QH
Liu
Jin-hui
JH
Jin
Lei
L
Yan
Li-xing
LX
D002681:China / Q000208:ethnology; D005006:Ethnicity; D005787:Gene Frequency; D005828:Genetics, Population; D006579:Heterozygote; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic; D020080:Tandem Repeat Sequences / Q000235:genetics*