Connectivity-based ab initio phasing at different solvent levels.
The connectivity-based phasing method has been applied independently to three neutron diffraction data sets obtained from the same crystal of tRNA(Asp)-aspartyl-tRNA synthetase complex but at different concentrations of D(2)O/H(2)O, thus masking different components of the crystal. The obtained low-resolution images correlate perfectly with the solvent level.
Fokine
Andrei
A
LCM3B, UMR 7036 CNRS, Faculté des Sciences, Université Henry Poincaré Nancy I, 54506 Vandoeuvre-lés-Nancy, France.
Lunina
Natalia
N
Lunin
Vladimir
V
Urzhumtsev
Alexandre
A
D001226:Aspartate-tRNA Ligase / Q000737:chemistry* / Q000378:metabolism; D017666:Deuterium Oxide / Q000737:chemistry; D008958:Models, Molecular; D033363:Neutron Diffraction / Q000379:methods*; D009690:Nucleic Acid Conformation; D011487:Protein Conformation; D012349:RNA, Transfer, Asp / Q000737:chemistry* / Q000378:metabolism; D012997:Solvents / Q000737:chemistry; D013223:Statistics as Topic / Q000379:methods; D014867:Water / Q000737:chemistry
D012349:RNA, Transfer, Asp; D012997:Solvents; D014867:Water; D001226:Aspartate-tRNA Ligase; D017666:Deuterium Oxide