title: Purification, nanocrystallization and preliminary X-ray analysis of a C-terminal part of tropomodulin protein 1, isoform A, from Caenorhabditis elegans.
abstract: The C-terminal part of tropomodulin protein 1, isoform A, from Caenorhabditis elegans was expressed in Escherichia coli and purified to homogeneity. Optimized from the initial nanoscreen, crystals grew to dimensions of 0.25 x 0.15 x 0.15 mm at 277 K using 28.0%(v/v) PEG 400 as the precipitant by the hanging-drop vapor-diffusion technique. A data set of 94.9% completeness was collected to a resolution of 1.98 A at 100 K using a synchrotron X-ray source (SER-CAT). The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 31.7, b = 50.6, c = 107.1 A, and contained one molecule per asymmetric unit.
authors.affiliation: Life Sciences College, Peking University, Beijing 100871, People's Republic of China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Ding
authors.forename: Shihong
authors.initials: S
authors.lastname: Qiu
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Bunzel
authors.forename: Danlin
authors.initials: D
authors.lastname: Luo
authors.forename: Alireza
authors.initials: A
authors.lastname: Arabashi
authors.forename: Shanyun
authors.initials: S
authors.lastname: Lu
authors.forename: Jindrich
authors.initials: J
authors.lastname: Symersky
authors.forename: Lisa A
authors.initials: LA
authors.lastname: Nagy
authors.forename: Lawrence J
authors.initials: LJ
authors.lastname: DeLucas
authors.forename: Songlin
authors.initials: S
authors.lastname: Li
authors.forename: Ming
authors.initials: M
authors.lastname: Luo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017173:Caenorhabditis elegans / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000378:metabolism; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D051348:Tropomodulin; D014961:X-Ray Diffraction
chemical_list: D029742:Caenorhabditis elegans Proteins; D011994:Recombinant Proteins; D051348:Tropomodulin; C480760:UNC-94 protein, C elegans