Mutations of the BAK gene are infrequent in advanced gastric adenocarcinomas in Koreans.
The bcl-2 homologue antagonist/killer (BAK) is a potently apoptosis-inducing gene and plays an important role in modulating apoptosis in epithelial cells. We have analyzed the mutation of the entire coding region of BAK gene in 107 Korean advanced gastric adenocarcinomas by polymerase chain reaction-single strand conformation polymorphism and sequencing. Homozygous deletions were not found in these samples. Only three cases of 107 gastric adenocarcinomas (2.8%) exhibited the BAK mutations. Two of them exhibited missense mutations and the remaining one had a silent mutation. All of these mutations were exclusively detected in exon 2. Mutations in the BAK gene were observed only in advanced gastric adenocarcinomas with extensive metastases of regional lymph nodes. The data presented here suggest that the mutations of BAK gene rarely occurred in advanced gastric adenocarcinomas.
Kim
Sang-Pyo
SP
Department of Pathology, School of Medicine, Keimyung University, #194 DongSan Dong Jung-Gu, 700-712 Daegu, South Korea.
Hwang
Mi-Sun
MS
Cho
Young Rok
YR
Kwon
Sun Young
SY
Kang
Yu-Na
YN
Kim
In-Ho
IH
Sohn
Soo-Sang
SS
Mun
Kyo-Cheol
KC
Kwon
Taeg-Kyu
TK
Lee
Seong-Ryong
SR
Suh
Seong-Il
SI
D000230:Adenocarcinoma / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology; D002288:Adenocarcinoma, Mucinous / Q000453:epidemiology / Q000235:genetics / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis / Q000235:genetics; D018279:Carcinoma, Signet Ring Cell / Q000453:epidemiology / Q000235:genetics / Q000473:pathology; D005091:Exons / Q000235:genetics; D005260:Female; D006801:Humans; D007723:Korea / Q000453:epidemiology; D008207:Lymphatic Metastasis; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D008875:Middle Aged; D020125:Mutation, Missense; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D013274:Stomach Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology; D050998:bcl-2 Homologous Antagonist-Killer Protein
C494772:BAK1 protein, human; D008565:Membrane Proteins; D050998:bcl-2 Homologous Antagonist-Killer Protein