Adenovirus-mediated functional gene transfer into parathyroid cells in vivo and in vitro.
Chronic renal failure patients usually develop secondary hyperparathyroidism and, as the disease progresses, there is a decrease in the number of vitamin D and calcium-sensing receptors (CaRs) in the parathyroid glands. Parathyroid cell function can be controlled if a functional gene is transferred into these cells using an adenovirus vector. Vitamin D or CaR genes transferred by the infected adenovirus vector induced a reduction in parathyroid hormone secretion. These results suggest that adenovirus-mediated gene transfer is a useful technique for control of parathyroid cell function.
Iwasaki
Yoshiko
Y
Division of Nephrology and Clinical Research Center, Tokyo Teishin Hospital, Tokyo, Japan. iwasakiy-tky@umin.ac.jp
Kakuta
Takatoshi
T
Haruguchi
Hiroaki
H
Fukuda
Naoko
N
Kurokawa
Kiyoshi
K
Fukagawa
Masafumi
M
D000256:Adenoviridae / Q000235:genetics*; D000257:Adenoviridae Infections / Q000378:metabolism / Q000473:pathology; D000818:Animals; D002478:Cells, Cultured; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006801:Humans; D008297:Male; D010280:Parathyroid Glands / Q000166:cytology / Q000378:metabolism; D010281:Parathyroid Hormone / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D018167:Receptors, Calcitriol / Q000235:genetics
D010281:Parathyroid Hormone; D018167:Receptors, Calcitriol