Vascular expression of matrix metalloproteinase-13 (collagenase-3) in basal cell carcinoma.
Matrix metalloproteinase-13 (MMP-13; collagenase-3) was detected in the vasculature from 17 of 20 human basal cell carcinomas as assessed by immunohistology immediately after surgery. In contrast, MMP-1 (interstitial collagenase) was detected in the vasculature of only two of the same specimens. MMP-13 reactivity was also observed in the capillaries of normal human skin taken from the wound margin. Human dermal microvascular endothelial cells as well as human umbilical vein endothelial cells were isolated in culture and examined for MMP-13 expression. By reverse transcription-polymerase chain reaction and Southern blotting, an MMP-13 transcript was detected in unstimulated endothelial cells. The transcript was upregulated in cells treated with 50 nM phorbol myristate acetate (PMA). Western blotting demonstrated the presence of an anti-MMP-13 - immunoreactive protein in culture fluid from both cell sources. Immunoreactivity was stronger in culture fluid from cells treated with interleukin-1alpha (IL-1alpha) than in culture fluid from control cells. Tumor necrosis factor-alpha (TNF-alpha) and PMA also upregulated MMP-13 expression but these agents were not as effective as IL-1alpha. Additionally, reactivity was greater in culture fluid from endothelial cells grown on three-dimensional lattices of polymerized type I collagen than on dried collagen films. These data indicate that endothelial cells in the skin are a source of MMP-13 and that enzyme expression is upregulated under conditions that promote endothelial cell growth and vascular differentiation.
Hattori
Yukari
Y
Department of Pathology, The University of Michigan, Ann Arbor 48109, USA.
Nerusu
Kamalakar C
KC
Bhagavathula
Narasimharao
N
Brennan
Meghan
M
Hattori
Noboru
N
Murphy
Hedwig S
HS
Su
Lyndon D
LD
Wang
Timothy S
TS
Johnson
Timothy M
TM
Varani
James
J
D015139:Blotting, Southern; D015153:Blotting, Western; D002196:Capillaries / Q000737:chemistry / Q000201:enzymology / Q000473:pathology; D002280:Basal Cell Carcinoma / Q000098:blood supply* / Q000473:pathology; D002455:Cell Division; D002478:Cells, Cultured; D017364:Collagenases / Q000235:genetics / Q000378:metabolism*; D017077:Culture Media, Conditioned / Q000737:chemistry; D020405:Dermis / Q000098:blood supply; D004730:Endothelium, Vascular / Q000187:drug effects / Q000201:enzymology*; D006801:Humans; D007124:Immunoenzyme Techniques; D053509:Matrix Metalloproteinase 13; D009924:Organ Culture Techniques; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012878:Skin Neoplasms / Q000098:blood supply* / Q000473:pathology / Q000601:surgery; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D017077:Culture Media, Conditioned; D012333:RNA, Messenger; D017364:Collagenases; C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13; D013755:Tetradecanoylphorbol Acetate