[Effect of antibacterial factors and cell metabolism biostimulants on bacterial luminescence].
Bioluminescence was used as an index of effects of a series of factors (differing in origin and structure) on cell metabolism. Luminescence of intact cells of Escherichia coli lum+ (a genetically modified strain) was measured on exposure to antibiotics, probiotics, phages, and biostimulators. The sensitivity of E. coli lum+ to antibiotics correlated with the antibiotic-induced luminescence quenching. Bioluminescence-based assessment of the antagonistic activity of the main probiotics (bificoll, bifidobacterin, acylact, colibacterin, and a composite probiotic preparation), each taken at a concentration of 1 dose/ml demonstrated that bacterial luminescence was inhibited by 75-99.9% (exposure time, 30 min). Bioluminescence changes reflected cell damage associated with phage infection. It was shown that bioluminescence stimulation could be used as an index of the effect of immunomodulators (olexin and vermin) on cell metabolism in bacterial cultures.
Pshenichnov
R A
RA
Institute of Ecology and Genetics of Microorganisms, Ural Division of the Russian Academy of Sciences, ul. Pushkina 1-57, Perm, 614081 Russia. pshenichnov@ecology.psu.ru
Maslennikova
I L
IL
Nikitina
N M
NM
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001430:Bacteriocins / Q000494:pharmacology; D003090:Coliphages; D004926:Escherichia coli / Q000187:drug effects / Q000502:physiology* / Q000821:virology; D007155:Immunologic Factors / Q000494:pharmacology*; D008156:Luciferases / Q000235:genetics; D008163:Luminescent Measurements; D019936:Probiotics / Q000494:pharmacology
D000276:Adjuvants, Immunologic; D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D007155:Immunologic Factors; D008156:Luciferases