title: [Screening of antimitotic portions from sea hare using conidia of Pyricularia Oryzae].
abstract: OBJECTIVE
  To apply conidia of Pyricularia Oryzae to the screening of antimitotic constituents from marine animal sea hare.
  
  
  METHODS
  To extract and fractionate active portions from sea hare through detecting deformation of mycelia germinated from conidia of P. Oryzae P-2b, in comparison with the cytotoxic test results in vitro.
  
  
  RESULTS
  Two active portions, of which IC50 against P388 and HL-60 was 23.4, 18.6 and 19.4, 12.5 micrograms.ml-1, respectively, were screened from this animal.
  
  
  CONCLUSIONS
  This bioassay method was efficiently applied to the primary screening of antimitotic portions from marine animals for the first time. Being convenient, speedy and cheap, the screening model is suitable for the bioassay of active constituents from marine life.
authors.affiliation: Shanghai Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
authors.forename: Hou-wen
authors.initials: HW
authors.lastname: Lin
authors.forename: Hai-feng
authors.initials: HF
authors.lastname: Tang
authors.forename: Chun
authors.initials: C
authors.lastname: Zhang
authors.forename: Yang
authors.initials: Y
authors.lastname: Shen
authors.forename: Cheng
authors.initials: C
authors.lastname: Guo
authors.forename: Yang-hua
authors.initials: YH
authors.lastname: Yi
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000494:pharmacology; D001048:Aplysia / Q000737:chemistry*; D018922:HL-60 Cells / Q000187:drug effects; D006801:Humans; D007941:Leukemia P388 / Q000473:pathology; D008419:Materia Medica / Q000302:isolation & purification* / Q000494:pharmacology; D051379:Mice; D008938:Mitosis / Q000187:drug effects*; D003904:Mitosporic Fungi / Q000502:physiology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D008419:Materia Medica