[Screening of antimitotic portions from sea hare using conidia of Pyricularia Oryzae].
OBJECTIVE
To apply conidia of Pyricularia Oryzae to the screening of antimitotic constituents from marine animal sea hare.


METHODS
To extract and fractionate active portions from sea hare through detecting deformation of mycelia germinated from conidia of P. Oryzae P-2b, in comparison with the cytotoxic test results in vitro.


RESULTS
Two active portions, of which IC50 against P388 and HL-60 was 23.4, 18.6 and 19.4, 12.5 micrograms.ml-1, respectively, were screened from this animal.


CONCLUSIONS
This bioassay method was efficiently applied to the primary screening of antimitotic portions from marine animals for the first time. Being convenient, speedy and cheap, the screening model is suitable for the bioassay of active constituents from marine life.
Lin
Hou-wen
HW
Shanghai Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
Tang
Hai-feng
HF
Zhang
Chun
C
Shen
Yang
Y
Guo
Cheng
C
Yi
Yang-hua
YH
D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000494:pharmacology; D001048:Aplysia / Q000737:chemistry*; D018922:HL-60 Cells / Q000187:drug effects; D006801:Humans; D007941:Leukemia P388 / Q000473:pathology; D008419:Materia Medica / Q000302:isolation & purification* / Q000494:pharmacology; D051379:Mice; D008938:Mitosis / Q000187:drug effects*; D003904:Mitosporic Fungi / Q000502:physiology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000970:Antineoplastic Agents; D008419:Materia Medica