[Effect of essential oil of Radix Angelicae Dahuricae on beta-endorphin, ACTH, NO and proopiomelanocortin of pain model rats].
OBJECTIVE
To investigate the effect of essential oil of Radix Aangelicae Dahuricae (EOAD) on beta-endorphin Adrenocorticotropic hormone(ACTH), Nitric oxide (NO) and Proopiomelanocortin (POMC) of pain model rats induced by formaldehyde.


METHODS
beta-endorphin and ACTH were detected with radio-immunity, the amount of masculine cell expression of Proopiomelanocortin mRNA in hypothalamus, and brainstem of pain model was detected with in situ hybridization and NO with chemical method.


RESULTS
EOAD could increase beta-endorphin, NO and the amount of masculine cell expression of Proopiomelanocortin mRNA significantly in hypothalamus and brainstem of rats.


CONCLUSIONS
EOAD which may be central analgesic, is associated with endogenous opiate-like substance such as POMC and beta-endorphin.
Nie
Hong
H
Nanjing University of Traditional Chinese Medicine, Nanjing 210029, Jiangsu, China. hongnie@hotmail.com
Shen
Ying-jun
YJ
D000324:Adrenocorticotropic Hormone / Q000097:blood; D000700:Analgesics / Q000302:isolation & purification / Q000494:pharmacology; D029969:Angelica / Q000737:chemistry; D000818:Animals; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D005557:Formaldehyde; D007031:Hypothalamus / Q000378:metabolism; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D009822:Oils, Volatile / Q000302:isolation & purification / Q000494:pharmacology*; D010146:Pain / Q000139:chemically induced / Q000378:metabolism*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011333:Pro-Opiomelanocortin / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D001615:beta-Endorphin / Q000378:metabolism*
D000700:Analgesics; D004365:Drugs, Chinese Herbal; D009822:Oils, Volatile; D012333:RNA, Messenger; D005557:Formaldehyde; D009569:Nitric Oxide; D001615:beta-Endorphin; D011333:Pro-Opiomelanocortin; D000324:Adrenocorticotropic Hormone