Comparative kinetic study of D-glucose oxidation by ruthenium(III) compounds catalyzed by FAD-dependent glucose oxidase and PQQ-dependent glucose dehydrogenase.
The comparative kinetic study of two glucose oxidizing enzymes, FAD-dependent glucose oxidase and PQQ-dependent glucose dehydrogenase, is presented in the artificial electron transfer mediator system based on ruthenium(III) compounds. It is demonstrated that FAD-dependent glucose oxidase and PQQ-dependent glucose dehydrogenase follow Michaelis kinetics in the D-glucose/ruthenium(III) system. PQQ-dependent glucose dehydrogenase is more active than FAD-dependent glucose oxidase in the process of D-glucose oxidation by ruthenium(III) compounds, this being due to the different catalytic mechanisms of these enzymes.
Ivanova
E V
EV
School of Chemistry, Lomonosov Moscow State University, Moscow, 119899, Russia. ivanka@enzyme.chem.msu.ru
Ershov
A Yu
AY
Laurinavicius
V
V
Meskus
R
R
Ryabov
A D
AD
D002384:Catalysis; D005947:Glucose / Q000737:chemistry* / Q000378:metabolism; D005948:Glucose Dehydrogenases / Q000737:chemistry* / Q000378:metabolism; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D015394:Molecular Structure; D010084:Oxidation-Reduction / Q000187:drug effects; D015363:Quinolones / Q000737:chemistry; D011809:Quinones / Q000737:chemistry; D012428:Ruthenium / Q000737:chemistry* / Q000494:pharmacology; D013237:Stereoisomerism; D013329:Structure-Activity Relationship; D013997:Time Factors
D015363:Quinolones; D011809:Quinones; D012428:Ruthenium; D005948:Glucose Dehydrogenases; D005949:Glucose Oxidase; D005947:Glucose