title: Independent recruitment in vivo by Gal4 of two complexes required for transcription.
abstract: We use a modified form of ChIP to analyze the recruitment of seven sets of proteins to the yeast GAL genes upon induction. We resolve three stages of recruitment: first SAGA, then Mediator, and finally Pol II along with four other proteins (including TBP) bind the promoter. In a strain lacking SAGA, Mediator is recruited with a time course indistinguishable from that observed in wild-type cells. Our results are consistent with the notion that a single species of activator, Gal4, separately contacts, and thereby directly recruits, SAGA and Mediator.
authors.affiliation: Molecular Biology Program, Sloan-Kettering Institute, New York, NY 10021, USA.
authors.forename: Gene O
authors.initials: GO
authors.lastname: Bryant
authors.forename: Mark
authors.initials: M
authors.lastname: Ptashne
mesh_terms: D001665:Binding Sites / Q000235:genetics; D002478:Cells, Cultured; D004268:DNA-Binding Proteins; D005786:Gene Expression Regulation / Q000235:genetics; D015966:Gene Expression Regulation, Fungal / Q000235:genetics*; D005809:Genes, Regulator / Q000235:genetics*; D046911:Macromolecular Substances; D056892:Mediator Complex; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012319:RNA Polymerase II / Q000235:genetics; D011930:Reaction Time / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D015534:Trans-Activators / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*; D015533:Transcriptional Activation / Q000235:genetics*; D015003:Yeasts / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; C058337:GAL11 protein, S cerevisiae; C052130:GAL4 protein, S cerevisiae; D046911:Macromolecular Substances; D056892:Mediator Complex; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D012319:RNA Polymerase II