[Effects of enteral nutrition on uptake of amino acid and enzyme-protein synthesis of pancreatic acinar cell in acute pancreatic dogs].
OBJECTIVE
To evaluate the effect of intrajejunal nutrition on uptake of amino acid and enzyme-protein synthesis in pancreatic acinar cell and subcellular fractionation and zymogen granules in dogs with acute pancreatitis.


METHODS
Fifteen dogs were induced acute pancreatitis by retrograde injection of 5% sodium-taurocholate into the pancreatic duct. Radioactive tracing and electron microscope were used to evaluate the change of amino acid uptake, enzyme-protein synthesis in acinar cell, subcellular fractionation, the quantitative analysis of mean zymogen granule number and mean zymogen granule area after injection L-(3)H-phenylalanine 30, 60, 120 1nd 180 min on the 7(th) day.


RESULTS
The radioactivity of L-(3)H phenylalanine uptake by pancreatic acinar cells and incorporations of L-(3)H phenylalanine into newly synthesized enzyme-protein peaked at 60 min. In enteral nutrition (EN) group it was higher that that in parenteral nutrition (PN) group (P < 0.05), and then gradually declined. The radioactivity peaked at 60 min in zymogen granule, lysosomal-mitochondria and microsomal subcellular fractionation. The latter two decreased, bat there was no significant difference (P > 0.05). The change of the mean number and mean area of zymogen granules were not significant different between the EN group and PN group (P > 0.05).


CONCLUSIONS
EN or PN do not stimulate pancreatic acinar uptake amino acid and enzyme-protein synthesis in acinar cell and subcellular fractionation.
Qin
Huan-long
HL
Department of Surgery, Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China.
Su
Zhen-dong
ZD
Ding
Zai-xian
ZX
Lin
Qing-tian
QT
D000208:Acute Disease; D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D004195:Disease Models, Animal; D004285:Dogs; D004750:Enteral Nutrition; D004792:Enzyme Precursors / Q000096:biosynthesis*; D005260:Female; D008297:Male; D046790:Pancreas, Exocrine / Q000378:metabolism; D010195:Pancreatitis / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D010288:Parenteral Nutrition; D011897:Random Allocation; D016896:Treatment Outcome
D000596:Amino Acids; D004792:Enzyme Precursors