Analysis of urinary nucleosides. III. Identification of 5'-deoxycytidine in urine of a patient with head and neck cancer.
Modified nucleosides in the urine have been postulated to be diagnostic indices of disease, particularly of cancer. The urine of a patient with terminal head and neck cancer has been found to contain a modified nucleoside with a protonated molecule of m/z 228. By means of high-performance liquid chromatography/ion trap mass spectrometry (HPLC/ITMS) and capillary liquid chromatography/triple quadruple mass spectrometry (CapLC/TQMS) we have identified the compound as 5'-deoxycytidine. This is the first report of 5'-deoxycytidine in man: in addition to the elucidation of its structure, its possible origins and the potential significance of its occurrence are discussed.
Dudley
Edward
E
Biochemistry Group, School of Biological Sciences, Wallace Building, University of Wales Swansea, Singleton Park, Swansea SA2 8PP, UK.
Lemiere
Filip
F
Van Dongen
Walter
W
Langridge
James I
JI
El-Sharkawi
Salah
S
Games
David E
DE
Esmans
Eddy L
EL
Newton
Russell P
RP
D002851:Chromatography, High Pressure Liquid; D003841:Deoxycytidine / Q000652:urine*; D006258:Head and Neck Neoplasms / Q000652:urine*; D006801:Humans; D013058:Mass Spectrometry
D003841:Deoxycytidine