Haem-oxygen reactive intermediates: catalysis by the two-step.
The catalytic schemes of a variety of haem enzymes, including the P450 mono-oxygenases, consist of a number of common reactive haem-oxygen adducts. The characterization of these intermediates by optical and EPR spectroscopies has reinforced the similarity of these intermediate states in a number of haem enzyme systems. Furthermore, the reactivity of these states in P450 and horseradish peroxidase, in which multiple potent oxidants are formed, provides a paradigm for many other haem enzymes.
Makris
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The Center for Biophysics and Computational Biology, University of Illinois Urbana-Champaign, 505 S. Goodwin Avenue, Urbana, IL 61801, USA.
Denisov
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Sligar
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D002384:Catalysis; D006418:Heme / Q000378:metabolism*; D007700:Kinetics; D016297:Mutagenesis, Site-Directed; D010100:Oxygen / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D013255:Steroid 21-Hydroxylase / Q000378:metabolism
D011994:Recombinant Proteins; D006418:Heme; D013255:Steroid 21-Hydroxylase; D010100:Oxygen