[Mutation of 5'noncoding region of the bcl-6 gene in diffuse large B cell lymphomas].
OBJECTIVE
To observe the mutation of 5'noncoding region of bcl-6 gene in diffuse large B cell lymphoma (DLBCL) and its effect on lymphoma pathogenesis.


METHODS
38 DLBCL, 2 reactive hyperplasias, 5 follicular lymphomas and 5 T cell lymphomas were chosen for PCR direct sequence analysis using two sets of primers in 5'noncoding region of the bcl-6 gene.


RESULTS
No mutation was found in the marginal region of reactive hyperplasias, T cell lymphomas, and follicular lymphomas but detected in 1/2 of the follicular center cells, and 7/38 cases of DLBCL. The incidence is less than that seen in other reports. Basepairs substitution and point insertion were the main mutation types.


CONCLUSIONS
The positive rate of mutation of 5'noncoding region of bcl-6 gene in DLBCL is 18.7%, less frequent than the published data of DLBCL reported in other countries. It may, in some extent, participate in the pathogenesis and progression of DLBCL.
Zhou
Xiao-yan
XY
Laboratory of Molecular Pathology, Cancer Hospital, Fudan University, Shanghai 200032, China.
Zhu
Wei-ping
WP
Zhang
Tai-ming
TM
Li
Xiao-mei
XM
Jin
Ai-ping
AP
Sun
Meng-hong
MH
Zhu
Xiong-zeng
XZ
D020121:5' Untranslated Regions / Q000235:genetics*; D044466:Asian People; D002681:China; D004268:DNA-Binding Proteins / Q000235:genetics*; D006801:Humans; D016393:Lymphoma, B-Cell / Q000235:genetics*; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics*; D017354:Point Mutation / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D051560:Proto-Oncogene Proteins c-bcl-6; D014157:Transcription Factors / Q000235:genetics*
D020121:5' Untranslated Regions; D004268:DNA-Binding Proteins; D011518:Proto-Oncogene Proteins; D051560:Proto-Oncogene Proteins c-bcl-6; D014157:Transcription Factors