Identification of SNPs, mapping and analysis of allele frequencies in two candidate genes for meat production traits: the porcine myosin heavy chain 2B (MYH4) and the skeletal muscle myosin regulatory light chain 2 (HUMMLC2B).
Myosin is one of the most important skeletal muscle proteins. It is composed of myosin heavy chains and myosin light chains that exist with different isoforms coded by different genes. We studied the porcine myosin heavy chain 2B (MYH4) and the porcine skeletal muscle myosin regulatory light chain 2 (HUMMLC2B) genes. A single nucleotide polymorphism (SNP), identified for each gene, was used for linkage mapping of MYH4 and HUMMLC2B to porcine chromosome (Sscr) 12 and Sscr 3, respectively. The mapping of these two genes was confirmed by using a porcine-rodent radiation hybrid panel, even if for MYH4 the LOD score and the retention fraction were low. Allele frequencies at the two loci were studied in a sample of 307 unrelated pigs belonging to seven different pig breeds. Moreover the distribution of the alleles at these two loci was analysed in groups of pigs with extreme divergent (positive and negative) estimated breeding values (EBV) for four meat production traits that have undergone selection in Italian heavy pigs.
Davoli
R
R
DIPROVAL--Sezione di Allevamenti Zootecnici, Faculty of Agriculture, University of Bologna, Via F.lli Rosselli 107, Villa Levi-Coviolo, 42100 Reggio Emilia, Italy. roberta.davioli@stpa.unibo.it
Fontanesi
L
L
Cagnazzo
M
M
Scotti
E
E
Buttazzoni
L
L
Yerle
M
M
Russo
V
V
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D005787:Gene Frequency / Q000235:genetics; D008460:Meat; D008969:Molecular Sequence Data; D018995:Myosin Heavy Chains / Q000235:genetics*; D018994:Myosin Light Chains / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D021101:Radiation Hybrid Mapping; D034421:Sus scrofa / Q000235:genetics*
D017931:DNA Primers; D018994:Myosin Light Chains; D018995:Myosin Heavy Chains