Deletion of scbA enhances antibiotic production in Streptomyces lividans.
Antibiotic production in many streptomycetes is influenced by extracellular gamma-butyrolactone signalling molecules. In this study, the gene scbA, which had been shown previously to be involved in the synthesis of the gamma-butyrolactone SCB1 in Streptomyces coelicolor A3(2), was deleted from the chromosome of Streptomyces lividans 66. Deletion of scbA eliminated the production of the antibiotic stimulatory activity previously associated with SCB1 in S. coelicolor. When the S. lividans scbA mutant was transformed with a multi-copy plasmid carrying the gene encoding the pathway-specific activator for either actinorhodin or undecylprodigiosin biosynthesis, production of the corresponding antibiotic was elevated significantly compared to the corresponding scbA(+) strain carrying the same plasmid. Consequently, deletion of scbA may be useful in combination with other strategies to construct host strains capable of improved bioactive metabolite production.
Butler
M J
MJ
Department of Molecular Microbiology, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK. michael.butler@bbsrc.ac.uk
Takano
E
E
Bruheim
P
P
Jovetic
S
S
Marinelli
F
F
Bibb
M J
MJ
D015107:4-Butyrolactone / Q000096:biosynthesis; D000880:Anthraquinones / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001426:Bacterial Proteins / Q000235:genetics*; D019149:Bioreactors; D004268:DNA-Binding Proteins / Q000235:genetics*; D005285:Fermentation; D017353:Gene Deletion; D005798:Genes, Bacterial; D010957:Plasmids / Q000235:genetics; D011353:Prodigiosin / Q000031:analogs & derivatives / Q000096:biosynthesis; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*; D000095622:Benzoisochromanequinones
D015107:4-Butyrolactone; D000880:Anthraquinones; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D011353:Prodigiosin; C446303:ScbA protein, Streptomyces; C047347:undecylprodigiosin; C013390:actinorhodin; D000095622:Benzoisochromanequinones