CD4(+)CD3(-) accessory cells costimulate primed CD4 T cells through OX40 and CD30 at sites where T cells collaborate with B cells.
In this report we identify an accessory cell that interacts with primed and memory T cells at sites where they collaborate with B cells. These cells are distinguished from conventional dendritic cells by their lack of response to Flt3 ligand and their inability to process antigen. Unlike dendritic cells, the CD4(+)CD3(-) cells have little CD80 or CD86 expression but do express high levels of the TNF ligands, OX40 ligand and CD30 ligand. We show that Th2-primed cells express the receptors for these TNF ligands and preferentially survive when cocultured with these cells. Furthermore, we show that the preferential survival of OX40(+) T cells and support of memory T cell help for B cells are linked to their association with CD4(+)CD3(-) cells in vivo.
Kim
Mi-Yeon
MY
Medical Research Council Centre for Immune Regulation, University of Birmingham Medical School, United Kingdom.
Gaspal
Fabrina M C
FM
Wiggett
Helen E
HE
McConnell
Fiona M
FM
Gulbranson-Judge
Adam
A
Raykundalia
Chandra
C
Walker
Lucy S K
LS
Goodall
Margaret D
MD
Lane
Peter J L
PJ
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018920:Coculture Techniques; D017730:Ki-1 Antigen / Q000276:immunology*; D008211:Lymphocyte Cooperation; D008562:Membrane Glycoproteins / Q000276:immunology*; D051379:Mice; D018613:Microscopy, Confocal; D053278:OX40 Ligand; D015398:Signal Transduction / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D048069:Tumor Necrosis Factors
D017730:Ki-1 Antigen; D008562:Membrane Glycoproteins; D053278:OX40 Ligand; C504586:Tnfsf4 protein, mouse; D048069:Tumor Necrosis Factors