title: Effect of bcl-2 antisense oligodeoxynucleotides on drug sensitivity of leukemic cells.
abstract: BACKGROUND
  We investigated the effect of two antisense oligodeoxynucleotides, previously selected with the help of computer-aided RNA structure prediction, on drug sensitivity, bcl-2 expression and apoptosis of leukemia cells. The drugs tested were etoposide (VP-16), cytarabine (Ara-C), daunorubicin (DNR) and arsenic trioxide (As(2)O(3)).
  
  
  METHODS
  The experimental assays were performed with cultures, IC(50) of leukemic cells to drugs, immunochemistry and flow cytometry.
  
  
  RESULTS
  The results showed that the two antisense oligodeoxynucleotides significantly reduced IC(50) levels for VP-16, Ara-c, DNA and As(2)O(3), inhibited bcl-2 gene expression and induced apoptosis of leukemic cells.
  
  
  CONCLUSIONS
  Computational prediction of antisense efficacy is faster than other methods and more cost-efficient. This could hasten the development of sequences for both research and clinical applications.
authors.affiliation: Institute of Hematology, Medical College of Jinan University, Guangzhou 510632, People's Republic of China. tzyuan@jnu.edu.cn
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiao Yong
authors.initials: XY
authors.lastname: Lei
mesh_terms: D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D004347:Drug Interactions; D019008:Drug Resistance, Neoplasm; D005434:Flow Cytometry; D018922:HL-60 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D020014:K562 Cells; D007938:Leukemia / Q000188:drug therapy* / Q000473:pathology; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis / Q000187:drug effects / Q000235:genetics*
chemical_list: D020319:Oligodeoxyribonucleotides, Antisense; D019253:Proto-Oncogene Proteins c-bcl-2