Designed structural-rearrangement of an active group I ribozyme.
The catalytic core of the Tetrahyemena group I ribozyme consists of two functionally different domains, P4-P6 and P3-P7, that are conjugated via multiple tertiary interactions. The sequence encoding the P3-P7 domain is divided into two fragments in its primary sequence although the two domains are physically separable in the three dimensional (3D-) structure of the ribozyme: The sequence encoding the P4-P6 domain is inserted into that of the P3-P7 domain. An artificial rearrangement was designed and attempted for the primary sequence of the P3-P7 domain on the basis of a 3D-structural model and the biochemical data on the ribozyme. The domain in the primary structure was relocated to form a contiguous region while retaining the 3D-structure of the ribozyme required for self-splicing. The topologically rearranged ribozyme exhibited self-splicing activity.
Ikawa
Yoshiya
Y
Graduate School of Biostudies, Kyoto University, Kyoto 606-8502, Japan.
Inoue
Tan
T
D000818:Animals; D001483:Base Sequence; D015195:Drug Design; D008958:Models, Molecular; D016296:Mutagenesis; D009154:Mutation; D009690:Nucleic Acid Conformation; D016337:RNA, Catalytic / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016808:Tetrahymena thermophila / Q000235:genetics*
C116167:GIR1 ribozyme; D016337:RNA, Catalytic