title: Uniform polarity microtubule assemblies imaged in native brain tissue by second-harmonic generation microscopy.
abstract: Microtubule (MT) ensemble polarity is a diagnostic determinant of the structure and function of neuronal processes. Here, polarized MT structures are selectively imaged with second-harmonic generation (SHG) microscopy in native brain tissue. This SHG is found to colocalize with axons in both brain slices and cultured neurons. Because SHG arises only from noninversion symmetric structures, the uniform polarity of axonal MTs leads to the observed signal, whereas the mixed polarity in dendrites leads to destructive interference. SHG imaging provides a tool to investigate the kinetics and function of MT ensemble polarity in dynamic native brain tissue structures and other subcellular motility structures based on polarized MTs.
authors.affiliation: School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Dombeck
authors.forename: Karl A
authors.initials: KA
authors.lastname: Kasischke
authors.forename: Harshad D
authors.initials: HD
authors.lastname: Vishwasrao
authors.forename: Martin
authors.initials: M
authors.lastname: Ingelsson
authors.forename: Bradley T
authors.initials: BT
authors.lastname: Hyman
authors.forename: Watt W
authors.initials: WW
authors.lastname: Webb
mesh_terms: D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D001921:Brain / Q000378:metabolism / Q000648:ultrastructure*; D006624:Hippocampus / Q000378:metabolism / Q000648:ultrastructure; D007150:Immunohistochemistry; D008856:Microscopy, Fluorescence / Q000379:methods*; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism / Q000648:ultrastructure*; D020409:Molecular Motor Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D017785:Photons; D051381:Rats; D017207:Rats, Sprague-Dawley; D014404:Tubulin / Q000378:metabolism; D016875:tau Proteins / Q000378:metabolism
chemical_list: D008869:Microtubule-Associated Proteins; D020409:Molecular Motor Proteins; D009419:Nerve Tissue Proteins; D014404:Tubulin; D016875:tau Proteins