title: [Study on stem-tip tissue culture of the traditional Chinese medicine Chrysanthemum morifolium].
abstract: OBJECTIVE
  To set up the optimums for the stem-tip tissue culture of Chrysanthemum morifolium cultivated in Anhui Province.
  
  
  METHODS
  Small sections (about 0.5 mm in length) from the stem-tips were isolated and inoculated with different media, and induced to form the whole plantlet formation.
  
  
  CONCLUSIONS
  The MS medium added with 6-BA 2 mg.L-1 + NAA 0.2 mg.L-1 was the optimum medium for the bud sprouting and the inducing rate was over 80% after 40 d cultivation on this modified medium. The MS medium supplemented with 6-BA 2 mg.L-1 and NAA 0.5 mg.L-1 was the optimum medium for the multiplication of the adventitious buds in which the bud multiplication was about 4-7 times higher after 25-30 d cultivation. The plantlet could root well on the MS medium with NAA 0.5 mg.L-1.
authors.affiliation: Department of Biology, Huaibei Coal Industry Teacher's College, Huaibei 235000, Anhui, China.
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Xue
authors.forename: Ai-min
authors.initials: AM
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Sheng
authors.forename: Feng-lan
authors.initials: FL
authors.lastname: Zhao
mesh_terms: D031202:Chrysanthemum / Q000254:growth & development*; D046508:Culture Techniques; D018547:Plant Stems / Q000254:growth & development