[The effect of kudingcha (Ilex latifolia Thunb.) on the contraction of isolated guinea-pig tracheal smooth muscle in vitro].
OBJECTIVE
To study the effects of Kudingcha (Ilex latifolia Thunb., IL) on the isolated guinea pig tracheal smooth muscle in vitro.


METHODS
By obtaining CaCl2 and Histamine accumulative dose-response curves, to observe the influences of IL on the contraction of tracheal strips induced by calcium and some asthmogenic mediators.


RESULTS
CaCl2 and histamine caused significant contraction of tracheal smooth muscle and pD2 was 3.55 and 5.34 respectively. After incubated with IL, the dose-response curves of CaCl2 and histamine were significantly shifted to the right, and the maximal contractile force was reduced. IL could also inhibit isolated tracheal strip contraction induced by acetylcholine 3 x 10(-6) mol.L-1 and histamine 3 x 10(-6) mol.L-1, and IC50 was 0.16 mg.ml-1 and 0.21 mg.ml-1.


CONCLUSIONS
Kudingcha has significant dilated effects on tracheal smooth muscle.
Jiang
J M
JM
Department of Pharmacy, Sun Yat-Sen University, Guangzhou 510275, Guangdong, China.
Xu
S B
SB
Kong
Y C
YC
D000818:Animals; D001993:Bronchodilator Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002122:Calcium Chloride / Q000037:antagonists & inhibitors; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D006168:Guinea Pigs; D006633:Histamine Antagonists / Q000494:pharmacology; D030017:Ilex / Q000737:chemistry*; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects*; D010946:Plants, Medicinal / Q000737:chemistry; D014132:Trachea / Q000187:drug effects
D001993:Bronchodilator Agents; D004365:Drugs, Chinese Herbal; D006633:Histamine Antagonists; D002122:Calcium Chloride