Identification and analysis of catalytic TIM barrel domains in seven further glycoside hydrolase families.
Fold recognition results allocate catalytic triose phosphate isomerase (TIM) barrels to seven previously unassigned glycoside hydrolase (GH) families, numbers 29, 44, 50, 71, 84, 85 and 89, enabling prediction of catalytic residues. Modelling of GH family 50 suggests that it may be the common evolutionary ancestor of families 42 and 14. TIM barrels now comprise the catalytic domains of more than half of the assigned GH families, and catalyse a much larger variety of GH reactions than any other catalytic domain architecture. Only 327 GH sequences still have no structurally identified catalytic domain.
Rigden
Daniel J
DJ
Embrapa Genetic Resources and Biotechnology, Cenargen/Embrapa, Estação Parque Biológico, Final W5, Asa Norte, 70770-900, Brasília-DF, Brazil. daniel@cenargen.embrapa.br
Jedrzejas
Mark J
MJ
de Mello
Luciane V
LV
D000595:Amino Acid Sequence; D020134:Catalytic Domain; D019143:Evolution, Molecular; D006026:Glycoside Hydrolases / Q000737:chemistry* / Q000235:genetics*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D012984:Software; D016963:Thermus thermophilus / Q000201:enzymology; D001616:beta-Galactosidase / Q000737:chemistry
D006026:Glycoside Hydrolases; D001616:beta-Galactosidase