[Hypoxia effects on vascular endothelial growth factor derived epithelial cells of nasal polyps].
OBJECTIVE
To understand the role of nasal mucous epithelial cells to hypoxia in early stage of nasal polyps(NP) formation.


METHODS
Epithelial cells of NP and inferior turbinate (IT) were cultured without serum under normal oxygen and hypoxia, and stimulus of inflammatory cytokines. Erythropoietin (EPO) was regarded as hypoxia mark, and expression of vascular endothelial growth factor(VEGF) mRNA and protein derived from epithelial cells were detected respectively by in situ hybridization and ELISA.


RESULTS
1. Under hypoxia, EPO mRNA was expressed intensely in epithelial cells from NP and IT, and there was no significant difference between both of them. This result suggested that EPO might be regarded as a hypoxic mark. 2. The ability of producing VEGF mRNA increased with cytokines stimulation, especially under hypoxia. Protein level of VEGF from epithelial cells of NP and IT increased with cytokines stimulation, especially in hypoxia and was time-dependent.


CONCLUSIONS
Epithelial cells actively produce vast VEGF under hypoxia. The VEGF induced by hypoxia of the mucosa in middle meatus is of importance in the formation of nasal polyps(NP) in early stage, which may be the major cause of NP formation in middle meatus.
Jiang
Shu
S
Department of Otorhinolaryngology, Head and Neck Surgery, China-Japan Union Hospital of Jilin University, Changchun 130031, China. jiangshu2@hotmail.com
Dong
Zhen
Z
Zhu
Dongdong
D
Yang
Zhanquan
Z
D015687:Cell Hypoxia; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000378:metabolism*; D004921:Erythropoietin / Q000096:biosynthesis; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis; D009298:Nasal Polyps / Q000378:metabolism* / Q000473:pathology; D014420:Turbinates / Q000378:metabolism; D042442:Vascular Endothelial Growth Factors / Q000096:biosynthesis* / Q000235:genetics
D007375:Interleukin-1; D042442:Vascular Endothelial Growth Factors; D004921:Erythropoietin