title: [mRNA expression of hyaluronan synthase in the endolymphatic sac cells of guinea pigs].
abstract: OBJECTIVE
  To detect the mRNA expression of hyaluronan synthase in the endolymphatic sac cells of guinea pigs.
  
  
  METHODS
  After consulting gene bank, we analyzed conservative sequence of hyaluronan synthases in different species, detected the mRNA expression of hyaluronan synthase in the endolymphatic sac cells of guinea pigs with oligonucleotide probe by hybridization in situ.
  
  
  RESULTS
  mRNA of hyaluronan synthase was strongly expressed in some epithelial cells of endolymphatic sac, coupled with negative expression in negative control groups.
  
  
  CONCLUSIONS
  It confirms that endolymphatic sac cells can synthesize hyaluronan.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China. zhonglm@cta.cq.cn
authors.forename: Z
authors.initials: Z
authors.lastname: Mou
authors.forename: Z
authors.initials: Z
authors.lastname: Liu
authors.forename: J
authors.initials: J
authors.lastname: Chen
authors.forename: Y
authors.initials: Y
authors.lastname: Wan
mesh_terms: D000818:Animals; D004712:Endolymphatic Sac / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D005260:Female; D014453:Glucuronosyltransferase / Q000096:biosynthesis* / Q000235:genetics; D016695:Glycosyltransferases; D006168:Guinea Pigs; D000076002:Hyaluronan Synthases; D066298:In Vitro Techniques; D008297:Male; D008565:Membrane Proteins; D012333:RNA, Messenger / Q000096:biosynthesis; D014166:Transferases; D029867:Xenopus Proteins
chemical_list: D008565:Membrane Proteins; D012333:RNA, Messenger; D029867:Xenopus Proteins; D014166:Transferases; D016695:Glycosyltransferases; D014453:Glucuronosyltransferase; C056944:HAS1 protein, Xenopus; D000076002:Hyaluronan Synthases