title: [Isolation of rat tracheal smooth muscle cells and electrophysiological examination of the L- type calcium channel].
abstract: OBJECTIVE
  To establish a method for acute enzymatic isolation of rat tracheal smooth muscle cells (TSMCs) and study the electrophysiological properties of the L-type calcium channel of the cells.
  
  
  METHODS
  Single rat TSMC was isolated by means of pronase E, followed by recording the electric currents in the single calcium channel using patch-clamp technique with cell attached configuration.
  
  
  RESULTS
  Large amount of viable TSMCs were isolated through this method, characterized by normal cell morphology and easy detection of the L-type calcium channel activity in the cells.
  
  
  CONCLUSIONS
  This method is relatively simple for high-yield isolation of TSMCs with normal morphology.
authors.affiliation: Department of Respiratory Diseases, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. dfzqrfll@yeah.net
authors.forename: Shi-hu
authors.initials: SH
authors.lastname: Cheng
authors.forename: Ya-ling
authors.initials: YL
authors.lastname: Luo
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Wen-yan
authors.initials: WY
authors.lastname: Lai
authors.forename: Liang
authors.initials: L
authors.lastname: Luo
mesh_terms: D000818:Animals; D020746:Calcium Channels, L-Type / Q000502:physiology*; D002469:Cell Separation / Q000379:methods*; D005260:Female; D008297:Male; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D014132:Trachea / Q000166:cytology*
chemical_list: D020746:Calcium Channels, L-Type