title: Effects of contrast media on erythrocyte aggregation during sedimentation.
abstract: To evaluate the effects of contrast media (CMs) on erythrocyte aggregation, we measured the erythrocyte sedimentation with Westergren method at 25 degrees C. CMs were diatrizoate (Urografin 76%) for ionic CM and iopamidol (Iopamiron 370) for nonionic CM. Swine red blood cells (RBCs) were suspended in autologous plasma containing diatrizoate (URO), iopamidol (IOP), and saline (SAL) at 6.7% w/w, as well as in plasma alone (PLA), at 40% of the hematocrit. Sigmoid sedimentation curves were fitted to the Puccini et al. (1977) equation, and the average number of RBCs per aggregate m was calculated by Stokes' law against the time t. According to the Murata-Secomb (1988) theory we estimated the collision rate K between two aggregates from dm/dt in the stationary phase during sedimentation. Corresponding to the maximal ESR, the dm/dt (in cells/s) was 0.52 in PLA, 0.09 in SAL, 0.06 in URO and 0.03 in IOP, so that K also decreased in proportion to dm/dt from 145 fL/s in PLA to 8 fL/s in IOP. Both the ionic and nonionic CMs tend to inhibit the RBC aggregation more than that in SAL; the latter iopamidol appears to be inhibitory more than the former diatrizoate in autologous plasma.
authors.affiliation: Department of Biological and Chemical Engineering, Faculty of Engineering, Gunma University, Kiryu, Gunma 376-8515, Japan.
authors.forename: Xuequn
authors.initials: X
authors.lastname: Huang
authors.forename: Akio
authors.initials: A
authors.lastname: Yoshikoshi
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Hirano
authors.forename: Akio
authors.initials: A
authors.lastname: Sakanishi
mesh_terms: D000818:Animals; D001799:Blood Sedimentation / Q000187:drug effects*; D003287:Contrast Media / Q000494:pharmacology*; D003973:Diatrizoate / Q000008:administration & dosage; D004903:Erythrocyte Aggregation / Q000187:drug effects*; D007479:Iopamidol / Q000008:administration & dosage; D016011:Normal Distribution; D010949:Plasma; D012965:Sodium Chloride; D013552:Swine; D013997:Time Factors
chemical_list: D003287:Contrast Media; D003973:Diatrizoate; D012965:Sodium Chloride; D007479:Iopamidol