Division site selection in Escherichia coli involves dynamic redistribution of Min proteins within coiled structures that extend between the two cell poles.
The MinCDE proteins of Escherichia coli are required for proper placement of the division septum at midcell. The site selection process requires the rapid oscillatory redistribution of the proteins from pole to pole. We report that the three Min proteins are organized into extended membrane-associated coiled structures that wind around the cell between the two poles. The pole-to-pole oscillation of the proteins reflects oscillatory changes in their distribution within the coiled structure. We also report that the E. coli MreB protein, which is required for maintaining the rod shape of the cell, also forms extended coiled structures, which are similar to the MreB structures that have previously been reported in Bacillus subtilis. The MreB and MinCDE coiled arrays do not appear identical. The results suggest that at least two functionally distinct cytoskeletal-like elements are present in E. coli and that structures of this type can undergo dynamic changes that play important roles in division site placement and possibly other aspects of the life of the cell.
Shih
Yu-Ling
YL
Department of Microbiology, University of Connecticut Health Center, Farmington, CT 06032, USA.
Le
Trung
T
Rothfield
Lawrence
L
D000251:Adenosine Triphosphatases / Q000096:biosynthesis / Q000502:physiology*; D018407:Bacterial Physiological Phenomena; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000502:physiology*; D018797:Cell Cycle Proteins; D002455:Cell Division; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000096:biosynthesis / Q000502:physiology*; D008856:Microscopy, Fluorescence; D008954:Models, Biological
D001426:Bacterial Proteins; D018797:Cell Cycle Proteins; D029968:Escherichia coli Proteins; C080466:MinC protein, Bacteria; C093548:MinE protein, E coli; C078473:MreB protein, E coli; D000251:Adenosine Triphosphatases; C072069:MinD protein, E coli