The birth of an alternatively spliced exon: 3' splice-site selection in Alu exons.
Alu repetitive elements can be inserted into mature messenger RNAs via a splicing-mediated process termed exonization. To understand the molecular basis and the regulation of the process of turning intronic Alus into new exons, we compiled and analyzed a data set of human exonized Alus. We revealed a mechanism that governs 3' splice-site selection in these exons during alternative splicing. On the basis of these findings, we identified mutations that activated the exonization of a silent intronic Alu.
Lev-Maor
Galit
G
Department of Human Genetics and Molecular Medicine, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv 69978, Israel.
Sorek
Rotem
R
Shomron
Noam
N
Ast
Gil
G
D000243:Adenosine Deaminase / Q000235:genetics; D017398:Alternative Splicing; D020087:Alu Elements / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D016373:DNA, Antisense; D015226:Dinucleoside Phosphates / Q000235:genetics; D005091:Exons; D015894:Genome, Human; D005964:Glucosyltransferases / Q000235:genetics; D006801:Humans; D007438:Introns; D016297:Mutagenesis, Site-Directed; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D000072260:RNA Splicing Factors; D016601:RNA-Binding Proteins; D017411:Ribonucleoproteins, Small Nuclear / Q000235:genetics / Q000502:physiology; D017381:Spliceosomes / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured
D016373:DNA, Antisense; D015226:Dinucleoside Phosphates; D000072260:RNA Splicing Factors; D016601:RNA-Binding Proteins; D017411:Ribonucleoproteins, Small Nuclear; C490656:SLU7 protein, human; C031924:adenylyl-(3'-5')-guanosine; D005964:Glucosyltransferases; C567955:ADARB1 protein, human; D000243:Adenosine Deaminase