Membrane topology of a metabotropic glutamate receptor.
The metabotropic glutamate receptors (mGluRs) have been predicted to have a classical seven transmembrane domain structure similar to that seen for members of the G-protein-coupled receptor (GPCR) superfamily. However, the mGluRs (and other members of the family C GPCRs) show no sequence homology to the rhodopsin-like GPCRs, for which this seven transmembrane domain structure has been experimentally confirmed. Furthermore, several transmembrane domain prediction algorithms suggest that the mGluRs have a topology that is distinct from these receptors. In the present study, we set out to test whether mGluR5 has seven true transmembrane domains. Using a variety of approaches in both prokaryotic and eukaryotic systems, our data provide strong support for the proposed seven transmembrane domain model of mGluR5. We propose that this membrane topology can be extended to all members of the family C GPCRs.
Bhave
Gautam
G
Division of Neuroscience, Baylor College of Medicine, Houston, Texas 77030, USA.
Nadin
Brian M
BM
Brasier
D J
DJ
Glauner
Kathi S
KS
Shah
Ruchir D
RD
Heinemann
Stephen F
SF
Karim
Farzana
F
Gereau
Robert W
RW
D000465:Algorithms; D000667:Ampicillin / Q000494:pharmacology; D000818:Animals; D019556:COS Cells; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D003001:Cloning, Molecular; D024881:Drug Resistance, Bacterial; D000939:Epitopes; D004926:Escherichia coli / Q000378:metabolism; D017353:Gene Deletion; D006031:Glycosylation; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D010455:Peptides / Q000737:chemistry; D017434:Protein Structure, Tertiary; D064529:Receptor, Metabotropic Glutamate 5; D018094:Receptors, Metabotropic Glutamate / Q000737:chemistry* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry; D014162:Transfection; D001618:beta-Lactamases / Q000378:metabolism
D000939:Epitopes; D010455:Peptides; D064529:Receptor, Metabotropic Glutamate 5; D018094:Receptors, Metabotropic Glutamate; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D000667:Ampicillin; D001618:beta-Lactamases