Potential osmoprotectants for the lactic acid bacteria Pediococcus pentosaceus and Tetragenococcus halophila.
The physiological responses of the lactic acid bacteria Pediococcus pentosaceus and Tetragenococcus halophila (formely known as P. halophila), subjected to osmotic stress in the presence of molecules known to act as osmoprotectants for other bacteria were studied. In a defined medium, glycine betaine, dimethylsulfonioacetate, choline, proline and L-carnitine were able to relieve inhibition of growth at 0.8 M NaCl. The five compounds were shown to efficiently compete with glycine betaine transport, suggesting the existence of common transporter(s) for these molecules. T. halophila, the most tolerant strain, exhibited a larger spectrum of compatible solutes including dimethylsulfonioacetate, dimethylsulfoniopropionate and ectoine. Preliminary data suggest that restoration of growth by ectoine under osmotic constraint seems specific to the genus Tetragenococcus.
Baliarda
Aurélie
A
Unité Sécurité Microbiologique des Aliments, ISTAB, Université Bordeaux 1, Avenue des Facultés, F-33405 Talence Cedex, France.
Robert
Hervé
H
Jebbar
Mohamed
M
Blanco
Carlos
C
Deschamps
Alain
A
Le Marrec
Claire
C
D000222:Adaptation, Physiological; D001622:Betaine / Q000378:metabolism / Q000494:pharmacology; D002331:Carnitine / Q000494:pharmacology; D002794:Choline / Q000494:pharmacology; D005516:Food Microbiology; D006863:Hydrogen-Ion Concentration; D009994:Osmolar Concentration; D009997:Osmotic Pressure; D010376:Pediococcus / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D011392:Proline / Q000378:metabolism / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013452:Sulfonium Compounds / Q000494:pharmacology
D013452:Sulfonium Compounds; D001622:Betaine; D012965:Sodium Chloride; C121105:dimethylsulfonioacetate; D011392:Proline; D002794:Choline; D002331:Carnitine