Quantification of elastin cross-linking amino acids, desmosine and isodesmosine, in hydrolysates of rat lung by ion-pair liquid chromatography-mass spectrometry.
The elastin cross-linking amino acids, desmosine (DES) and isodesmosine (IDE), in hydrolysates of rat lungs were quantified by ion-pair liquid chromatography-electrospray mass spectrometry. The column was a 2.0 mm i.d. x 150 mm Develosil UG3 (ODS) with a mobile phase A of 7 mM pentafluoropropionic anhydride (PFPA) using ultrapure water as the ion-pair reagent, and a mobile phase B of 7 mM PFPA in 80% methanol. The retention times of IDE and DES were 25.5 and 26.6 min, respectively. The mean concentrations of IDE and DES in the lung were 191.6+/-54.5 nmol/g lung (dry tissue) (+/-SD) and 184.0+/-39.3 nmol/g lung, respectively, and the IDE/DES ratio was 1.04, in Wistar Kyoto rats. Our results indicate that ion-pair liquid chromatography-mass spectrometry is a useful procedure for quantitation of DES and IDE in hydrolysates of rat lung.
Kaga
Naoko
N
Central Laboratory of Medical Sciences, Juntendo University School of Medicine, Tokyo, Japan.
Soma
Sanae
S
Fujimura
Tsutomu
T
Seyama
Kuniaki
K
Fukuchi
Yoshinosuke
Y
Murayama
Kimie
K
D000818:Animals; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D003895:Desmosine / Q000032:analysis*; D004549:Elastin / Q000032:analysis / Q000737:chemistry*; D007524:Isodesmosine / Q000032:analysis*; D008168:Lung / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D051381:Rats
D003895:Desmosine; D004549:Elastin; D007524:Isodesmosine