title: Species differences in the susceptibility of erythrocytes exposed to free radicals in vitro.
abstract: The susceptibility of human, cow, pig, sheep and rabbit erythrocytes to free radicals (peroxyl radicals) generated in vitro by 2,2'-azo-bis(2-amidinopropane) hydrochloride (AAPH) was evaluated by means of a haemolysis test and expressed as the time to 50% of maximal haemolysis (HT50). The most sensitive to damage by free radicals appeared to be the erythrocytes of pigs and sheep, their HT50 values being (mean +/- SEM) 85.1 +/- 1.28 min and 89.0 +/- 1.31 min, respectively. Human erythrocytes and those of cows and rabbits were about twice as resistant, their HT50 values being (mean +/- SEM) 174.3 +/- 1.53 min, 181.2 +/- 1.22 min and 183.4 +/- 2.54 min, respectively. Pig and sheep erythrocytes used in the haemolysis test provided an indication of the antioxidant status in a shorter time (2.5 h versus 4.5 h) than those of the other species studied. The results indicate that the HT50 test may be a convenient alternative to the osmotic resistance test for defining the antioxidant resistance of erythrocytes.
authors.affiliation: Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences in Olsztyn, Department of Hormonal Action Mechanisms, ul. Grunwaldzka 250, 60-166 Poznań, Poland. endocrin@rose.man.poznan.pl
authors.forename: E
authors.initials: E
authors.lastname: Brzezińska-Slebodzińska
mesh_terms: D000328:Adult; D000578:Amidines / Q000097:blood / Q000633:toxicity*; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D006461:Hemolysis; D006801:Humans; D018384:Oxidative Stress / Q000502:physiology; D010545:Peroxides / Q000097:blood / Q000633:toxicity*; D011817:Rabbits; D012756:Sheep; D013045:Species Specificity; D013552:Swine
chemical_list: D000578:Amidines; D010545:Peroxides; C049375:perhydroxyl radical; C046728:2,2'-azobis(2-amidinopropane)