[Analysis of splicing variants in NASG 3'UTR, down-regulated in nasopharyngeal carcinoma, and its expression in multiple cancer tissues].
OBJECTIVE
NASG gene, a tissue-specific gene of human nasopharyngeal epithelium was isolated by suppression subtractive hybridization. This study was designed to analyze splicing variants in NASG 3'untranslated region (UTR) and its expression profiling in multiple cancer tissues.


METHODS
The PCR primers were designed in NASG 3'UTR around the splicing variants and reverse transcription-polymerase chain reaction (RT-PCR) was performed. The PCR products were separated and sequenced. The expression patterns of NASG were detected by RT-PCR among nasopharyngeal carcinoma (NPC) cell line HNE1, primary human embryo nasopharyngeal epithelial cells, NPC biopsies, and normal adult nasopharyngeal epithelial tissues. Its expression profiling in multiple cancer tissues were tested by cancer profiling array hybridization.


RESULTS
There were three splicing variants in NASG 3'UTR. NASG was identified to be down-regulated in NPC cell line HNE1 and 71% of the NPC biopsies, but up-regulated in 25% lung of the cancer biopsies, and not express in other cancer tissues and normal tissues.


CONCLUSIONS
There were three splicing variants in NASG 3'UTR. Its abnormal expression may be an important molecular event in NPC and lung cancer.
Zhang
Bi-Cheng
BC
Cancer Research Institute, Xiangya Medical School,Central South University, Changsha, Hunan, PR China.
Zhu
Shi-Guo
SG
Xiang
Juan-Juan
JJ
Zhou
Ming
M
Nie
Xin-Min
XM
Xiao
Bing-Yi
BY
Li
Xiao-Ling
XL
Li
Gui-Yuan
GY
D020413:3' Untranslated Regions / Q000235:genetics*; D000328:Adult; D017398:Alternative Splicing; D001483:Base Sequence; D004273:DNA, Neoplasm / Q000032:analysis; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA
D020413:3' Untranslated Regions; C485599:BPIFA1 protein, human; D004273:DNA, Neoplasm; D006023:Glycoproteins; D009363:Neoplasm Proteins; D010750:Phosphoproteins