title: Variations in metabolism of the soy isoflavonoid daidzein by human intestinal microfloras from different individuals.
abstract: Isoflavonoids found in legumes, such as soybeans, are converted by intestinal bacteria to metabolites that might have increased or decreased estrogenic activity. Variation in the effects of dietary isoflavonoids among individuals has been attributed to differences in their metabolism by intestinal bacteria. To investigate this variation, the metabolism of the isoflavonoid daidzein by bacteria from ten fecal samples, provided at different times by six individuals on soy-containing diets, was compared. After anaerobic incubation of bacteria with daidzein for 2 weeks, four samples had metabolized daidzein and six samples had not. Three of the positive samples were from individuals whose microflora had not metabolized daidzein in previous samples. Dihydrodaidzein was observed in one sample, dihydrodaidzein and equol in another sample, and equol and O-desmethylangolensin in two other samples. These results corroborate the hypothesis that the microflora of the gastrointestinal tract of an individual influences the particular isoflavone metabolites produced following consumption.
authors.affiliation: Division of Microbiology, National Center for Toxicological Research, U.S. FDA, Jefferson, AR 72079, USA. frafii@nctr.fda.gov
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Rafii
authors.forename: Christy
authors.initials: C
authors.lastname: Davis
authors.forename: Miseon
authors.initials: M
authors.lastname: Park
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Heinze
authors.forename: Richard D
authors.initials: RD
authors.lastname: Beger
mesh_terms: D000693:Anaerobiosis; D004032:Diet; D060754:Equol; D005243:Feces / Q000382:microbiology*; D006094:Gram-Positive Bacteria / Q000378:metabolism*; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D007529:Isoflavones / Q000737:chemistry / Q000378:metabolism*; D013025:Glycine max / Q000737:chemistry*
chemical_list: C007610:4',7-dihydroxy-3,4-dihydroisoflavone; D007529:Isoflavones; D060754:Equol; C004742:daidzein