Cloning and sequence analyses of a 2,3-dihydroxybiphenyl 1,2-dioxygenase gene ( bphC) from Comamonas sp. SMN4 for phylogenetic and structural analysis.
A genomic library of biphenyl-degrading Comamonas sp. SMN4 for isolating fragments containing the 2,3-dihydroxybiphenyl 1,2-dioxygenase (23DBDO) gene was constructed. The smallest subclone (pNPX9) encoding 23DBDO activity was sequenced and analyzed. The C-terminal domain of 23DBDO from Comamonas sp. SMN4 had five catalytically essential residues and was more highly conserved than the N-terminal domain. Phylogenetic and structural relationships of 23DBDO from Comamonas sp. SMN4 were analyzed.
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Korea Food Research Institute, Baekhyon, Poondang, Songnam, 463-746, Kyongki-do, Korea.
Kwon
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Min
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D001713:Biphenyl Compounds / Q000378:metabolism; D003001:Cloning, Molecular; D020585:Comamonas / Q000145:classification / Q000201:enzymology* / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis; D049308:Dioxygenases; D004785:Environmental Pollutants / Q000378:metabolism; D007218:Industrial Microbiology; D010105:Oxygenases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D017434:Protein Structure, Tertiary
D001713:Biphenyl Compounds; D004269:DNA, Bacterial; D049308:Dioxygenases; D004785:Environmental Pollutants; D010105:Oxygenases; C010574:biphenyl; C074272:2,3-dihydroxybiphenyl oxygenase