[Induction of callus of Hypericum perforatum L. and qualitative identification of active constituents of its callus].
OBJECTIVE
To induce the callus of H. perforatum and identify hypericin and pseudohypericin of its callus.


METHODS
The callus was induced in different culture conditions, and active constituents were determined by HPLC.


CONCLUSIONS
The inductions of callus from different parts were discussed, the induction rate of the leaf axil being the highest. The MS basic medium with 4 micrograms.L-1 2,4-D and 0.2 microgram.L-1 6-BA was the best of all screened media. Hypericin in the callus is determined by HPLC.
Xu
M S
MS
Institute of Chinese Materia Medica, Academy of Traditional Chinese Medicine, Beijing 100700.
Huang
L Q
LQ
Fu
M H
MH
Te
H C
HC
Li
G F
GF
D000873:Anthracenes; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D020902:Hypericum / Q000737:chemistry / Q000254:growth & development*; D010569:Perylene / Q000031:analogs & derivatives* / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry* / Q000254:growth & development*
D000873:Anthracenes; D003470:Culture Media; D010569:Perylene; C004965:hypericin; C056602:pseudohypericin