title: [Genetic polymorphism of cytochrome P4501A1 and susceptibility to laryngeal carcinoma].
abstract: OBJECTIVE
  To assess the possible relation between the MspI polymorphism of Cytochrome P4501A1(CYP1A1) gene and the susceptibility to laryngeal cancer.
  
  
  METHODS
  The genotypes of CYP1A1 MspI site were detected using the methods of polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) in 62 cases of laryngeal squamous carcinoma and 56 healthy controls. Genetic risk of CYP1A1 genotypes was analyzed by smoking index (SI, cigarettes smoked per day x years of smoking).
  
  
  RESULTS
  Three genotypes of CYP1A1 MspI were classified into the predominant homozygotes (A), heterozygotes (B) and the rare homozygotes (C). The frequency of heterozygote B (58.1%) and genotype C (14.5%) in the patients with laryngeal cancer were higher than that of the controls (39.3%, 7.1%, P < 0.05), while their odds ratios were 2.89 (95% confidence interval, CI: 1.31-6.37) and 3.97 (95% CI: 1.10-14.28), respectively. The odds ratios of genotype C was 9 (95% CI: 1.60-50.74) in the high dose cigarette smoking group, but was 4.5 (95% CI: 0.64-31.61) in the low dose cigarette smoking group.
  
  
  CONCLUSIONS
  With the carcinogenesis and development of laryngeal cancer, the polymorphism of CYP1A1 gene and smoking exposure together may play an important role. The individuals with genotype C are at especially high risk of laryngeal cancer, which grows with increasing cigarette consumption.
authors.affiliation: Department of Otorhinolaryngology, Qilu Hospital, Shandong University, Jinan 250012, China. leidapengcn@yahoo.com.cn
authors.forename: Dapeng
authors.initials: D
authors.lastname: Lei
authors.forename: Xinliang
authors.initials: X
authors.lastname: Pan
authors.forename: Chenhong
authors.initials: C
authors.lastname: Guo
authors.forename: Xinyong
authors.initials: X
authors.lastname: Luan
authors.forename: Fenglei
authors.initials: F
authors.lastname: Xu
authors.forename: Liqiang
authors.initials: L
authors.lastname: Zhang
authors.forename: Dayu
authors.initials: D
authors.lastname: Liu
mesh_terms: D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012907:Smoking / Q000009:adverse effects
chemical_list: D019363:Cytochrome P-450 CYP1A1