Estimation of the proportion of body fat in mice from the proportion of body water.
We compared the proportion of body fat in mice as measured by chemical analysis with that estimated from the proportion of body water. First, we measured the proportion of fat by chemical analysis in 78 mice that had a proportion of body fat in the range from approximately 5 to 20%. Then, we constructed a regression line that described the relationship between the proportion of body water and the proportion of body fat by using data from several other published studies in mice (% body fat = -1.20 x % body water + 88.07, r = 0.9597, sy.x = 2.75, p < 0.001). With this regression line, we estimated the proportion of body fat from the proportion of body water that was measured by drying the carcasses at 60 degrees C for 3 days. Body fat data obtained from this regression line were similar to those obtained by chemical analysis. Thus, these results suggest that reliable values for the proportion of body fat can be derived from the proportion of body water and this method provides a tool to rapidly measure the proportion of body fat in mice.
Terpstra
A H M
AH
Department of Laboratory Animal Science, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 2, 3584 CM Utrecht, The Netherlands. a.h.m.terpstra@las.vet.uu.nl
D000273:Adipose Tissue / Q000033:anatomy & histology*; D000818:Animals; D001823:Body Composition; D001834:Body Water; D008055:Lipids / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D012044:Regression Analysis; D015203:Reproducibility of Results
D008055:Lipids