[Optimization of conditions for storage and cultivation of the fungus Claviceps sp.--a producer of the ergot alkaloid agroclavine].
Conditions of agroclavine biosynthesis by the mutant Claviceps sp. strain s 106 were studied. The content of agroclavine was maximum (1.5-2 g/l) on days 15-16 of cultivation in the complex medium T25, containing sucrose, citric acid, and yeast extract. Agroclavine was the major component of the alkaloid fraction (90-95%). Storage of the culture at -70 degrees C in T25 supplemented by 7% glycerol provided a stable level of alkaloid formation.
Boĭchenko
L V
LV
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Oblast, 142290 Russia.
Zelenkova
N F
NF
Arinbasarov
M U
MU
Reshetilova
T A
TA
D000388:Air; D019343:Citric Acid; D002967:Claviceps / Q000254:growth & development / Q000378:metabolism*; D015925:Cryopreservation; D003470:Culture Media; D004873:Ergolines / Q000378:metabolism*; D004876:Ergot Alkaloids / Q000096:biosynthesis*; D013395:Sucrose; D013997:Time Factors; D015003:Yeasts
D003470:Culture Media; D004873:Ergolines; D004876:Ergot Alkaloids; D019343:Citric Acid; D013395:Sucrose; C004465:agroclavine