Profile and clinical significance of anti-nuclear envelope antibodies found in patients with primary biliary cirrhosis: a multicenter study.
Primary biliary cirrhosis (PBC) sera contain antibodies which recognize various nuclear envelope proteins of which antibody against gp210 has been proven to be diagnostic for disease. In contrast, the clinical significance of another nuclear envelope antibody, anti-p62 antibody has not been well investigated. In the present study, we have analyzed anti-nuclear envelope antibodies by indirect immunofluorescence and immunoblot using rat liver nuclear envelope proteins and wheat germ agglutinin-bound fraction. Test sera were obtained from 175 patients with PBC and from 120 controls. Anti-gp210, anti-lamina associated polypeptide 2, anti-lamin B receptor, and anti-p62 complex antibodies were detected with a frequency of 26% (46 of 175), 6% (11 of 175), 9% (16 of 175), and 13% (15 of 115), respectively. The confirmation of Scheuer's stage IV was made with a frequency of 27% (4 of 15) in PBC patients with anti-p62 complex antibody, in contrast to only 2% (2 of 100) in PBC patients without anti-p62 complex antibody. This difference was found to be statistically significant. The presence of anti-p62 complex antibody may be related with the progressive or advanced state of PBC.
Miyachi
Kiyomitsu
K
First Diagnostic Division, Health Sciences Research Institute Inc, 106 Godo-cho Hodogaya-ku, Yokohama-shi, Kanagawa 240-0005, Japan. keigu@miyachik.or.jp
Hankins
Raleigh W
RW
Matsushima
Hiroshi
H
Kikuchi
Futoshi
F
Inomata
Tetushi
T
Horigome
Tuneyoshi
T
Shibata
Minoru
M
Onozuka
Yasushi
Y
Ueno
Yukihisa
Y
Hashimoto
Etsuko
E
Hayashi
Naoaki
N
Shibuya
Akitaka
A
Amaki
Shuichi
S
Miyakawa
Hiroshi
H
D000818:Animals; D000974:Antibodies, Antinuclear / Q000097:blood*; D016022:Case-Control Studies; D004268:DNA-Binding Proteins / Q000276:immunology; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D015151:Immunoblotting; D066298:In Vitro Techniques; D008105:Liver Cirrhosis, Biliary / Q000276:immunology* / Q000473:pathology; D008297:Male; D008562:Membrane Glycoproteins / Q000276:immunology; D008565:Membrane Proteins / Q000276:immunology; D008875:Middle Aged; D009685:Nuclear Envelope / Q000276:immunology*; D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins / Q000276:immunology; D051381:Rats; D018160:Receptors, Cytoplasmic and Nuclear / Q000276:immunology; D000097682:Lamin B Receptor
D000974:Antibodies, Antinuclear; D004268:DNA-Binding Proteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins; D018160:Receptors, Cytoplasmic and Nuclear; D000097682:Lamin B Receptor; C063865:NUP210 protein, human; C093413:lamina-associated polypeptide 2; C049312:nuclear pore protein p62