title: Improving heterologous gene expression in Aspergillus niger by introducing multiple copies of protein-binding sequence containing CCAAT to the promoter.
abstract: OBJECTIVE
  To increase the expression level of heterologous gene in Aspergillus niger.
  
  
  RESULTS
  Inserting multiple copies of an activator protein-binding site originally encoded in the cis-regulatory region of A. niger glucoamylase gene (glaA), CCAAT-containing sequence, into the promoter of an expression plasmid greatly enhanced the production of heterologous protein.
  
  
  CONCLUSIONS
  The promoter activity was increased by the introduction of the CCAAT-containing sequence resulting in increased gene expression.
  
  
  CONCLUSIONS
  This study provides evidence that the CCAAT-containing region of the glaA promoter of A. niger is a binding site for a positive transcriptional factor and demonstrates the possibility for improving the expression of both heterologous and endogenous genes in Aspergillus.
authors.affiliation: Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China.
authors.forename: L
authors.initials: L
authors.lastname: Liu
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.forename: R X
authors.initials: RX
authors.lastname: Qiu
authors.forename: X G
authors.initials: XG
authors.lastname: Zhu
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Dong
authors.forename: G M
authors.initials: GM
authors.lastname: Tang
mesh_terms: D001234:Aspergillus niger / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D018628:Gene Dosage; D015966:Gene Expression Regulation, Fungal; D005087:Glucan 1,4-alpha-Glucosidase / Q000737:chemistry* / Q000235:genetics; D006454:Hemoglobins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011995:Recombination, Genetic; D014170:Transformation, Genetic; D054793:Truncated Hemoglobins
chemical_list: D001426:Bacterial Proteins; D006454:Hemoglobins; D054793:Truncated Hemoglobins; C082613:hemoglobin protein, Vitreoscilla; D005087:Glucan 1,4-alpha-Glucosidase