[Study on a new point mutation of nt3426 A --> G of mitochondrial DNA in a diabetes mellitus family].
OBJECTIVE
To elucidate the relationship between point mutations of nt3243A --> G, nt3426 A --> G of mitochondrial DNA and type 2 diabetes mellitus(DM).


METHODS
Two hundred patients with type 2 DM and 180 controls with normal glucose tolerance and absence of DM family history were included. The mutations were determined by PCR-restriction fragment length polymorphism.


RESULTS
The point mutation nt3426A --> G of mitochondrial DNA ND1 was found in 2 of the patients with type 2 DM (1.0%) but in none of the controls (0). The incidence of this mutation showed no significant difference between the two groups(P>0.05). And none was found to have the mutation of nt3243 --> G.


CONCLUSIONS
The point mutation nt3426 A --> G of mitochondrial DNA ND1 may not be an independent factor to cause type 2 DM.
Yu
Pei
P
Department of Endocrinology, Metabolic Diseases Hospital, Tianjin Medical University, Tianjin, 300070 PR China.
Yu
De-min
DM
Liu
De-min
DM
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004272:DNA, Mitochondrial / Q000032:analysis / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005190:Family; D006801:Humans; D008297:Male; D008875:Middle Aged; D010375:Pedigree; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D055815:Young Adult
D004272:DNA, Mitochondrial