Determination by HPLC of chlortetracycline in pig faeces.
An HPLC assay used to determine chlortetracycline (CTC) in pig faeces is reported. Prodigy ODS3 (4.6 x 150 mm) was used for the stationary phase, whereas the mobile phase comprised oxalic acid, sodium oxalate and sodium decane sulfonate (66%)--each of 4 mM, and 34% acetonitrile. The mobile phase was pumped at a flow rate of 1 mL/min. Detection of CTC was by ultraviolet absorbance at 370 nm, and a 20 micro L injection volume was used. Recovery from faeces was >90%, and coefficients of variability between runs were <10%. The lowest limit of quantification was 3.5 mg/kg, with an accuracy of <7% error. There was no interference from endogenous materials in the pig faeces, or commonly used antibiotics, and the method is suitable for use in drug disposition studies.
Sunderland
Julie
J
Bristol Centre for Antimicrobial Research and Evaluation, North Bristol NHS Trust, Southmead Hospital, Bristol BS10 5NB, UK. sunderland_j@southmead.swest.nhs.uk
Lovering
Andrew M
AM
Tobin
Caroline M
CM
MacGowan
Alasdair P
AP
Roe
John M
JM
Delsol
Anne A
AA
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002751:Chlortetracycline / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005243:Feces / Q000737:chemistry*; D015203:Reproducibility of Results; D013552:Swine
D000900:Anti-Bacterial Agents; D002751:Chlortetracycline