Genetic structure along a gaseous organic pollution gradient: a case study with Poa annua L.
The population genetic composition of Poa annua L. was studied by starch electrophoresis along a transect running NE from an organic reagents factory at Shanghai, China. Five enzyme systems were stained. We have reached the following preliminary conclusions: (1). Organic pollution has dramatically changed genotypic frequencies at some loci of Poa annua populations. At polluted sites, significant deviations from Hardy-Weinberg equilibrium were observed on loci Sod-1 and Me due to the excess of heterozygote. Especially in the two nearest sites to pollution source, all the individuals were heterozygous at locus Sod-1. The data suggests that heterozygotes were more tolerant to organic pollution than homozygotes, indicating the fitness superiority of heterozygotes. (2). A tendency towards clinal changes of allele frequencies was found at some polymorphic loci. Frequencies of the common alleles at loci Sod-1, Me and Fe-1 increased as the distance to the pollution source increased. (3). The effective number of alleles per locus, and the observed and expected heterozygosity were much higher in the pollution series than in the clear control site (Botanic Park population), but genetic multiplicity (number of alleles per locus) was lower than for the control. (4). Most genetic variability was found within populations, and only 2.56% were among populations of the polluted series. However, 9.48% of the total genetic variation occurred among populations when including the Botanic Park population. The genetic identity between populations of the pollution series (0.9869-1.0000, mean 0.9941) was higher than those between the pollution series and the Botanic Park population. UPGMA divided the five populations into two groups. One contained the four polluted populations, and the other only contained the Botanic Park population.
Chen
Xiao-yong
XY
Department of Environmental Sciences, East China Normal University, Zhongshan Road (N) 3663, Shanghai 200062, PR China. xychens@public4.sta.net
Li
Ning
N
Shen
Lang
L
Li
Yuan-yuan
YY
D000393:Air Pollutants / Q000009:adverse effects*; D000483:Alleles; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000235:genetics; D002617:Chemical Industry; D002681:China; D017343:Genes, Plant / Q000187:drug effects*; D014644:Genetic Variation; D005838:Genotype; D006579:Heterozygote; D008291:Malate Dehydrogenase / Q000235:genetics; D009930:Organic Chemicals / Q000009:adverse effects*; D010734:Phosphogluconate Dehydrogenase / Q000235:genetics; D031785:Poa / Q000187:drug effects* / Q000235:genetics; D013482:Superoxide Dismutase / Q000235:genetics; D000072105:Superoxide Dismutase-1
D000393:Air Pollutants; D009930:Organic Chemicals; D008291:Malate Dehydrogenase; D010734:Phosphogluconate Dehydrogenase; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase