title: The IS1 elements in Shigella boydii: horizontal transfer, vertical inactivation and target duplication.
abstract: IS1(SB) and its two variants were identified as the major and minor IS1 elements in Shigella boydii. The nucleotide sequences of IS1(SB), IS1(O157:H7) from Escherichia coli O157:H7 and IS1F from E. coli K12 suggest that these IS1 elements had been horizontally transferred among S. boydii and E. coli O157:H7 and K12. The two IS1(SB) variants and IS1(O157:H7) have transposition activities 7- to 86-fold less than that of IS1(SB), whereas IS1F has little transposition activity. Analysis of the flanking sequences of IS1(SB) and its two variants in S. boydii revealed the nature of regional specificity of the target sites and the sequence dependence of 8 and 9 bp target duplications, for which a model is presented.
authors.affiliation: Institute of Molecular Biology, National Chung Hsing University, 402, Taichung, Taiwan.
authors.forename: Wen-Bin
authors.initials: WB
authors.lastname: Hsu
authors.forename: Jiann-Hwa
authors.initials: JH
authors.lastname: Chen
mesh_terms: D024509:3' Flanking Region; D024506:5' Flanking Region; D001483:Base Sequence; D016384:Consensus Sequence; D004251:DNA Transposable Elements; D004926:Escherichia coli; D019453:Escherichia coli O157; D022761:Gene Transfer, Horizontal / Q000235:genetics*; D009154:Mutation; D012761:Shigella boydii / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements