[Effects of sodium salicylate on outward potassium current, resting potential and membrane electric capacitance of the outer hair cell isolated from guinea pig cochlea].
OBJECTIVE
To study effects of sodium salicylate on the outward potassium current (IK), resting potential (RP) and membrane electric capacitance (MEC) of outer hair cells isolated from guinea pig cochleas and the cytoelectrophysiological mechanisms of effects of sodium salicylate on functions of hair cells.


METHODS
Using the patch-clamp whole cell recording technique, IK, RP and MEC were measured before and after the administration of sodium salicylate at concentration of 0.1 or 1.0 mmol/L. The effects of sodium salicylate on IK, RP and MEC were observed.


RESULTS
The effects of sodium salicylate on IK had time- and dosage-effect relations, of which the former mean IK increasing and then decreasing after administration, the later meant that the effects of sodium salicylate being much more dominant during either increasing or decreasing IK in higher concentration than in lower concentration. The mean RP was approximately--60 mV and the mean MEC was approximately 39 pF. Sodium salicylate could decrease RP and MEC, which possessed a dosage-effect relation, that is, the effect was stronger in higher concentration than in lower concentration, and there was no clear evidence for time-effect relation.


CONCLUSIONS
The sodium salicylate might affect the K+ conduction at the lateral membrane and internal and external distribution of K+ in the outer hair cell. Sodium salicylate might affect the excitability and mechanical activity of the outer hair cell by means of affecting IK, RP and MEC, which might be one of the cochlear mechanisms of the effects of sodium salicylate on the functions of hair cells.
Liang
Y
Y
Department of Otorhinolaryngology, Union Hospital, Tongji Medical University, Wuhan 430022.
Zhong
N
N
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D025802:Electric Capacitance; D005260:Female; D006168:Guinea Pigs; D018072:Hair Cells, Auditory, Outer / Q000187:drug effects* / Q000502:physiology; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology*; D012980:Sodium Salicylate / Q000494:pharmacology*
D015221:Potassium Channels; D012980:Sodium Salicylate