Orthotopic implantation of a colon cancer xenograft induces high expression of cyclooxygenase-2.
The aim of the present study is to investigate the relation of cyclooxygenase (COX)-2 with liver metastasis. Pieces of human colon cancer xenograft were implanted orthotopically (CI), as well as intraperitoneally, and subcutaneously. Liver metastasis developed most frequently, and the COX-2 expression of both mRNA and protein of the tumors was the most dominant in the CI group. In contrast, the expression of COX-1 or vascular endothelial cell growth factor mRNAs had no significant differences among the groups. The intensity of COX-2 mRNA was negatively correlated with the apoptotic index. In conclusion, COX-2 plays an important role in organ-specific metastasis of the colon cancer.
Shoji
Tuyoshi
T
Department of Surgery II, Hamamatsu University School of Medicine, 1-20-1 Handa-yama, Hamamatsu-shi, Shizuoka, 431-3192, Japan. tsyoji@hama-med.ac.jp
Konno
Hiroyuki
H
Tanaka
Tatsuo
T
Sakaguchi
Takanori
T
Sunayama
Kenichi
K
Baba
Megumi
M
Kamiya
Kinji
K
Ohta
Manabu
M
Kaneko
Takeshi
T
Igarashi
Akira
A
Nakamura
Satoshi
S
D000230:Adenocarcinoma / Q000098:blood supply / Q000201:enzymology / Q000473:pathology / Q000556:secondary*; D000818:Animals; D017209:Apoptosis; D002432:Cecum; D003110:Colonic Neoplasms / Q000201:enzymology / Q000473:pathology*; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D016228:Endothelial Growth Factors / Q000096:biosynthesis / Q000235:genetics; D004790:Enzyme Induction; D015972:Gene Expression Regulation, Neoplastic; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis / Q000235:genetics; D007527:Isoenzymes / Q000096:biosynthesis* / Q000235:genetics; D008113:Liver Neoplasms / Q000098:blood supply / Q000201:enzymology / Q000556:secondary*; D008222:Lymphokines / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D008565:Membrane Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D009368:Neoplasm Transplantation / Q000379:methods*; D009389:Neovascularization, Pathologic; D009928:Organ Specificity; D010529:Peritoneal Cavity; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012334:RNA, Neoplasm / Q000096:biosynthesis / Q000235:genetics; D012867:Skin; D013047:Specific Pathogen-Free Organisms; D014183:Transplantation, Heterologous; D016042:Transplantation, Heterotopic; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D016228:Endothelial Growth Factors; D036341:Intercellular Signaling Peptides and Proteins; D007527:Isoenzymes; D008222:Lymphokines; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases; C496512:Ptgs1 protein, mouse