Improved recovery of DNA from polyacrylamide gels after in situ DNA footprinting.
Methods used to date for the isolation of DNA from polyacrylamide gels are elution based, time-consuming and with low yield in DNA. This paper describes an improved system employing polyacrylamide gels made of a meltable matrix. The new system was successfully applied to in situ DNA footprinting following gel retardation assays.
van Keulen
Geertje
G
Department of Microbiology, Groningen Biotechnology and Biomolecular Science Institute (GBB), University of Groningen, PO Box 14, 9750 AA, Haren, The Netherlands. G.van.Keulen@biol.rug.nl
Meijer
Wim G
WG
D000180:Acrylic Resins; D004247:DNA / Q000302:isolation & purification*; D018983:DNA Footprinting; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D015203:Reproducibility of Results
D000180:Acrylic Resins; C016680:polyacrylamide gels; D004247:DNA