Electron transfer in human cytochrome P450 reductase.
Cytochrome P450 reductase (CPR) is a diflavin enzyme responsible for electron donation to mammalian cytochrome P450 enzymes in the endoplasmic reticulum. Dissection of the enzyme into functional domains and studies by site-directed mutagenesis have enabled detailed characterization of the mechanism of electron transfer using stopped-flow and equilibrium-perturbation methods, and redox potentiometry. These studies and the mechanism of electron transfer in CPR are reported herein.
Gutierrez
A
A
Department of Biochemistry, University of Leicester, University Road, Leicester LE1 7RH, UK.
Grunau
A
A
Paine
M
M
Munro
A W
AW
Wolf
C R
CR
Roberts
G C K
GC
Scrutton
N S
NS
D000595:Amino Acid Sequence; D004579:Electron Transport; D006801:Humans; D007700:Kinetics; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism*; D014364:Tryptophan
D014364:Tryptophan; D009251:NADPH-Ferrihemoprotein Reductase