title: [Absorption and distribution of 5-aminosalicylic acid from its chitosan capsule degraded by colon bacteria-released enzymes in rats].
abstract: OBJECTIVE
  To examine the absorption and distribution of 5-aminosalicylic acid (5-ASA) in rat colon after oral administration of its chitosan capsule.
  
  
  METHODS
  5-ASA in chitosan capsules (4.8 mg per 3 capsules) were administered orally in rats via a polyethylene cannula under light ether anesthesia. The rats in control group were given 1 ml 5-ASA carboxymethyl cellulose suspension (4.8 mg). Blood samples were obtained from the rat hearts and the colon tissues sampled at a given interval to measure the concentration of 5-ASA by HPLC.
  
  
  RESULTS
  The area under the curve (AUC(0-12)) in the serum samples of chitosan capsule group was 0.62 times that of the control group, while in the colon tissue, the AUC(0-12) of chitosan capsule group was as much as 3.62 times that of the control group.
  
  
  CONCLUSIONS
  The chitosan capsule may be a useful carrier of 5-ASA for colon-specific delivery.
authors.affiliation: Department of Pharmaceutics, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. lgfnf@fimmu.com
authors.forename: Guo-feng
authors.initials: GF
authors.lastname: Li
authors.forename: Jian-hai
authors.initials: JH
authors.lastname: Chen
authors.forename: Li-jie
authors.initials: LJ
authors.lastname: Liu
authors.forename: Dan
authors.initials: D
authors.lastname: Guo
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yan
authors.forename: Zhong-yuan
authors.initials: ZY
authors.lastname: Xu
authors.forename: Zhi-liang
authors.initials: ZL
authors.lastname: Chen
mesh_terms: D000042:Absorption; D000818:Animals; D019540:Area Under Curve; D001419:Bacteria / Q000201:enzymology; D002686:Chitin / Q000008:administration & dosage* / Q000031:analogs & derivatives*; D048271:Chitosan; D002851:Chromatography, High Pressure Liquid; D003106:Colon / Q000378:metabolism* / Q000382:microbiology; D019804:Mesalamine / Q000008:administration & dosage* / Q000493:pharmacokinetics; D051381:Rats; D014018:Tissue Distribution
chemical_list: D002686:Chitin; D019804:Mesalamine; D048271:Chitosan