title: The age-related accumulation of dolichol in rat liver may correlate with expectation of life.
abstract: In order to test the hypothesis that the ageing-related alteration in membrane lipids might reflect the biological age of rodents, the levels of liver dolichol were assayed by the HPLC procedure in male ad-libitum fed (AL) Sprague-Dawley rats aged 2, 6, 12 and 24 months, and in 24-month-old rats on anti-aging food-restrictions (FR) differing in duration and in their effects on longevity. Results showed that the effects on liver dolichol of FR initiated at 2, 6 and 12 months of age, or initiated at 2 and interrupted at 18 months of age were significantly different, and reflected the differences in the effects of FR on expectation of life (the longer the expected residual lifespan the lower the content in liver dolichol). The conclusion is that assay of the quantity of dolichol in the liver tissue may be used as a marker of the biological age of the animal and therefore as an important biomarker of ageing.
authors.affiliation: Dipartimento di Patologia Sperimentale, Biotecnologie Mediche, Infettivologia e Epidemiologia, University of Pisa, Pisa, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Dolfi
authors.forename: Ettore
authors.initials: E
authors.lastname: Bergamini
authors.forename: Cristiano
authors.initials: C
authors.lastname: Carresi
authors.forename: Gabriella
authors.initials: G
authors.lastname: Cavallini
authors.forename: Alessio
authors.initials: A
authors.lastname: Donati
authors.forename: Marco
authors.initials: M
authors.lastname: Maccheroni
authors.forename: Ilaria
authors.initials: I
authors.lastname: Parentini
authors.forename: Maria
authors.initials: M
authors.lastname: Marino
authors.forename: Zina
authors.initials: Z
authors.lastname: Gori
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D002784:Cholesterol / Q000378:metabolism; D004032:Diet; D004286:Dolichols / Q000378:metabolism*; D004435:Eating; D008017:Life Expectancy; D008099:Liver / Q000378:metabolism*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004286:Dolichols; D002784:Cholesterol