Preparative purification of the major anti-inflammatory triterpenoid esters from Marigold (Calendula officinalis).
A method for the efficient preparative purification of faradiol 3-O-laurate, palmitate and myristate, the major anti-inflammatory triterpenoid esters in the flower heads of the medicinal plant Calendula officinalis has been developed. Gram quantities of the individual compounds were obtained with 96 to 98% purity by a combination of supercritical fluid extraction (SFE), normal-phase and reversed-phase column chromatography. During the work-up of the faradiol esters, accompanying minor compounds of the triterpene ester fraction were purified and identified by spectroscopic means as maniladiol 3-O-laurate and myristate.
Hamburger
M
M
Institute of Pharmacy, Friedrich-Schiller-University Jena, Semmelweisstrasse 10, Jena D-07743, Germany. b7hama@rz.uni-jena.de
Adler
S
S
Baumann
D
D
Förg
A
A
Weinreich
B
B
D000893:Anti-Inflammatory Agents / Q000737:chemistry*; D020838:Calendula; D002851:Chromatography, High Pressure Liquid; D004952:Esters / Q000737:chemistry; D035263:Flowering Tops; D006801:Humans; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry*; D014315:Triterpenes / Q000737:chemistry*
D000893:Anti-Inflammatory Agents; D004952:Esters; D010936:Plant Extracts; D014315:Triterpenes