[The study of p16 gene mutation and methylation in laryngeal squamous cell carcinoma].
OBJECTIVE
To investigate the development and mechanism of the p16 gene inactivation in laryngeal squamous cell carcinoma (LSCC).


METHODS
With PCR-based methylation assay and PCR-SSCP, first and second exon mutation in p16 gene and methylation of endogenase in promoter in 32 cases of LSCC and paracancer tissue were studied.


RESULTS
No mutation were found in first and second exon in the samples; methylation of Cfo I were detected in 6 cases in first exon, methylation of Sac II was found in 4 cases, and methylation of Hpa II in 4 cases. In second exon, methylation of Hpa II was detected in 5 cases and methylation of Cfo I in 6 cases.


CONCLUSIONS
Methylation of promoter in p16 gene is one of the important mechanisms of the inactivation of the gene. The inactivation of p16 gene has a close relation with the development and progress of LSCC.
Bai
S
S
Department of Otorhinolaryngology, Second Clinical College, Chinese Medical University, Shenyang 110003, China. baisu@pub.sy.ln.cn
Gao
H
H
Liang
H
H
Fei
S
S
Zhang
X
X
Sun
K
K
D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D019175:DNA Methylation; D005091:Exons; D019942:Genes, p16; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics*; D009154:Mutation