title: The VirR/VirS regulatory cascade affects transcription of plasmid-encoded putative virulence genes in Clostridium perfringens strain 13.
abstract: We analyzed the transcriptional regulation of the putative virulence genes encoded on the plasmid pCP13 from Clostridium perfringens strain 13. The transcription of the beta2-toxin (cpb2) and possible collagen adhesin (cna) genes were regulated in both a positive and negative manner, respectively, by the two-component VirR/VirS system. The secondary regulator of the VirR/VirS system, VR-RNA, also affects the expression of both of these genes in the same fashion as the VirR/VirS system. This indicates that the global regulatory cascade of the VirR/VirS system controls the expression of virulence genes located on the plasmid, as well as those found chromosomally in C. perfringens strain 13.
authors.affiliation: Department of Infection Biology, Institute of Basic Medical Sciences, University of Tsukuba, 1-1-1, Tennohdai, Ibaraki Tsukuba 305-8575, Japan.
authors.forename: Kaori
authors.initials: K
authors.lastname: Ohtani
authors.forename: Hameem I
authors.initials: HI
authors.lastname: Kawsar
authors.forename: Kayo
authors.initials: K
authors.lastname: Okumura
authors.forename: Hideo
authors.initials: H
authors.lastname: Hayashi
authors.forename: Tohru
authors.initials: T
authors.lastname: Shimizu
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D001427:Bacterial Toxins / Q000235:genetics; D001483:Base Sequence; D003016:Clostridium perfringens / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D014158:Transcription, Genetic; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; C075216:Clostridium novyi alpha-toxin; D012329:RNA, Bacterial; C109896:cpb2 protein, Clostridium perfringens; C068267:mry protein, Streptococcus pyogenes