The role of abiotic factors and pesticide levels on enzymatic activity in the freshwater mussel Anodonta cygnea at three different exposure sites.
Natural variation in abiotic factors, such as temperature and pH, probably influence the activity of enzymes used as potential biomarkers in bivalve mollusks to assess environmental contamination in the field. Changes in levels of an enzymatic biomarker may thus merely reflect natural variation in the annual physiological cycle of a species rather than exposure to contaminants. To investigate this issue, we documented the relationship between pesticide levels in water and three different enzymatic biomarkers over 1 year in enclosed populations of the freshwater unionid mussel Anodonta cygnea at three different sites of exposure. We considered the natural variation in temperature, pH and dissolved oxygen over the year and across the different sites as a potential correlate of enzymatic activity to disentangle the relative contribution of abiotic factors and pesticide levels. Pesticide levels varied among the three sites and over the course of the year. Catalase (CAT) and acetylcholinesterase activity (AChE) varied as a function of abiotic factors but showed no relation to pesticide levels. Glutathione S-transferase (GST) activity was also related to abiotic factors but also decreased with increases in total pesticide levels. The lack of activity induction or inhibition by pesticides and the natural variation in abiotic factors among sites and across time limits the use of CAT and AChE to assess environmental contamination in this species.
Robillard
Sophie
S
Laboratoire de Biologie et Génétique Evolutive, Université du Maine, Le Mans, France.
Beauchamp
Guy
G
Laulier
Marc
M
D000110:Acetylcholinesterase / Q000032:analysis; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D049872:Bivalvia / Q000187:drug effects / Q000201:enzymology*; D002374:Catalase / Q000032:analysis; D004781:Environmental Exposure / Q000009:adverse effects / Q000032:analysis*; D004784:Environmental Monitoring / Q000379:methods*; D005602:France; D005618:Fresh Water; D005982:Glutathione Transferase / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D010088:Oxidoreductases / Q000032:analysis* / Q000187:drug effects; D010100:Oxygen / Q000032:analysis; D010575:Pesticides / Q000009:adverse effects / Q000032:analysis*; D013696:Temperature; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000009:adverse effects / Q000032:analysis*
D015415:Biomarkers; D010575:Pesticides; D014874:Water Pollutants, Chemical; D010088:Oxidoreductases; D002374:Catalase; D005982:Glutathione Transferase; D000110:Acetylcholinesterase; D010100:Oxygen