title: Comparative roles of the cell wall and cell membrane in limiting uptake of xenobiotic molecules by Saccharomyces cerevisiae.
abstract: Using reversible electropermeabilization of cells and spheroplasts, we show that the cell wall and plasma membrane partly account for bleomycin resistance by acting as two independent barriers. We also report on the presence of a membrane protein that may be responsible for bleomycin internalization and toxicity in Saccharomyces cerevisiae.
authors.affiliation: Laboratoire Vectorologie et Transfert de Gènes, UMR 8121 CNRS, Institut Gustave-Roussy, 94805 Villejuif Cédex, France.
authors.forename: Mustapha
authors.initials: M
authors.lastname: Aouida
authors.forename: Omar
authors.initials: O
authors.lastname: Tounekti
authors.forename: Omrane
authors.initials: O
authors.lastname: Belhadj
authors.forename: Lluis M
authors.initials: LM
authors.lastname: Mir
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000493:pharmacokinetics*; D001761:Bleomycin / Q000493:pharmacokinetics*; D002462:Cell Membrane / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000187:drug effects / Q000502:physiology; D002473:Cell Wall / Q000378:metabolism; D025141:Drug Resistance, Fungal; D018274:Electroporation; D005656:Fungal Proteins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*; D013104:Spheroplasts / Q000502:physiology
chemical_list: D000903:Antibiotics, Antineoplastic; D005656:Fungal Proteins; D008565:Membrane Proteins; D001761:Bleomycin