title: Rapid urease screening of Yersinia on CIN agar plates.
abstract: Yersinia enterocolitica is an important foodborne pathogen, but isolation of virulent Yersinia from food sources is still time consuming and requires skills. In this article, we describe a rapid urease screening on cefsulodin-irgasan-novobiocin (CIN) agar plates with an agar overlay assay. This test is simple to perform, all colonies on a plate can be checked simultaneously, it only takes minutes for detection of urease-positive colonies and the colonies survive for transfer, further characterisation, and storage. Additionally, this method is useful to isolate virulent (urease-positive and pYV harbouring) Y. enterocolitica from foodstuffs.
authors.affiliation: Institute of Meat Hygiene, Meat Technology and Food Science, University of Veterinary Medicine Vienna, Veterinaerplatz 1, A-1210 Vienna, Austria. friederike.hilbert@vu-wien.ac.at
authors.forename: Friederike
authors.initials: F
authors.lastname: Hilbert
authors.forename: Sigrid
authors.initials: S
authors.lastname: Mayrhofer
authors.forename: Frans J M
authors.initials: FJ
authors.lastname: Smulders
mesh_terms: D000362:Agar; D002224:Carbanilides / Q000378:metabolism; D002441:Cefsulodin / Q000378:metabolism; D003470:Culture Media; D004791:Enzyme Inhibitors / Q000378:metabolism; D009675:Novobiocin / Q000378:metabolism; D013997:Time Factors; D014510:Urease / Q000378:metabolism*; D015008:Yersinia enterocolitica / Q000201:enzymology* / Q000302:isolation & purification
chemical_list: D002224:Carbanilides; D003470:Culture Media; D004791:Enzyme Inhibitors; D009675:Novobiocin; D000362:Agar; D014510:Urease; C005055:cloflucarban; D002441:Cefsulodin