Genetic diversity of a canine coronavirus detected in pups with diarrhoea in Italy.
The sequence of the S gene of a field canine coronavirus (CCoV), strain Elmo/02, revealed low nucleotide (61%) and amino acid (54%) identity to reference CCoV strains. The highest correlation (77% nt and 81.7% aa) was found with feline coronavirus type I. A PCR assay for the S gene of strain Elmo/02 detected analogous CCoVs of different geographic origin, all which exhibited at least 92-96% nucleotide identity to each other and to strain Elmo/02. The evident genetic divergence between the reference CCoV strains and the newly identified Elmo/02-like CCoVs strongly suggests that a novel genotype of CCoV is widespread in the dog population.
Pratelli
Annamaria
A
Department of Animal Health and Well-being, Faculty of Veterinary Medicine of Bari, S.p. per Casamassima km 3, 70010, Valenzano, Bari, Italy. a.pratelli@veterinaria.uniba.it <a.pratelli@veterinaria.uniba.it>
Martella
Vito
V
Decaro
Nicola
N
Tinelli
Antonella
A
Camero
Michele
M
Cirone
Francesco
F
Elia
Gabriella
G
Cavalli
Alessandra
A
Corrente
Marialaura
M
Greco
Grazia
G
Buonavoglia
Domenico
D
Gentile
Mattia
M
Tempesta
Maria
M
Buonavoglia
Canio
C
D000595:Amino Acid Sequence; D000818:Animals; D002415:Cats; D018352:Coronavirus Infections / Q000662:veterinary* / Q000821:virology; D017939:Coronavirus, Canine / Q000145:classification / Q000235:genetics*; D016765:Coronavirus, Feline / Q000235:genetics; D003967:Diarrhea / Q000662:veterinary* / Q000821:virology; D004283:Dog Diseases / Q000821:virology*; D004285:Dogs; D005243:Feces; D014644:Genetic Variation; D005838:Genotype; D007558:Italy; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics*
D008562:Membrane Glycoproteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins