Characterization of mammalian selenoproteomes.
In the genetic code, UGA serves as a stop signal and a selenocysteine codon, but no computational methods for identifying its coding function are available. Consequently, most selenoprotein genes are misannotated. We identified selenoprotein genes in sequenced mammalian genomes by methods that rely on identification of selenocysteine insertion RNA structures, the coding potential of UGA codons, and the presence of cysteine-containing homologs. The human selenoproteome consists of 25 selenoproteins.
Kryukov
Gregory V
GV
Department of Biochemistry, University of Nebraska, Lincoln, NE 68588-0664, USA.
Castellano
Sergi
S
Novoselov
Sergey V
SV
Lobanov
Alexey V
AV
Zehtab
Omid
O
Guigó
Roderic
R
Gladyshev
Vadim N
VN
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003062:Codon; D018388:Codon, Terminator; D019295:Computational Biology; D004251:DNA Transposable Elements; D020869:Gene Expression Profiling; D015894:Genome, Human; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011506:Proteins / Q000737:chemistry* / Q000235:genetics*; D020543:Proteome; D051381:Rats; D012643:Selenium; D017279:Selenocysteine / Q000737:chemistry / Q000235:genetics*; D051140:Selenoproteins; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012984:Software
D003062:Codon; D018388:Codon, Terminator; D004251:DNA Transposable Elements; D011506:Proteins; D020543:Proteome; D051140:Selenoproteins; D017279:Selenocysteine; D012643:Selenium