title: Cloning the full-length cDNA of a gene responsible for vascular smooth muscle cell proliferation in atherogenesis and study of function.
abstract: OBJECTIVE
  To clone the full-length cDNA of a gene responsible for vascular smooth muscle cell (v-SMC) proliferation in atherogenesis, and study its function.
  
  
  METHODS
  Oxidized low density lipoprotein (ox-LDL) at optimal concentration was used as the stimulant to induce v-SMC proliferation in culture medium. A cDNA subtractive library of v-SMC proliferation specific to ox-LDL stimulation was established using subtractive hybridization technique. Methods, including blotting, Northern hybridization and gene sequencing, were used to clone new gene fragments. By using full-length cDNA screening and protein expression techniques, one full-length cDNA was cloned and its function was studied.
  
  
  RESULTS
  One full-length cDNA was cloned. The new gene (Genbank AF 174647) expressed a 44 kDa protein, which might be associated with the activity of ox-LDL.
  
  
  CONCLUSIONS
  The new gene cloned may be associated with SMC proliferation in atherogenesis.
authors.affiliation: Department of Chest Surgery, The First Hospital of Henan Medical University, Zhengzhou 450052, China.
authors.forename: Gaofeng
authors.initials: G
authors.lastname: Zhao
authors.forename: Peng
authors.initials: P
authors.lastname: Gao
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.forename: Liming
authors.initials: L
authors.lastname: Lou
authors.forename: Huihong
authors.initials: H
authors.lastname: Qiao
authors.forename: Mingpeng
authors.initials: M
authors.lastname: She
mesh_terms: D000595:Amino Acid Sequence; D001161:Arteriosclerosis / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003001:Cloning, Molecular; D015723:Gene Library; D006801:Humans; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000187:drug effects*; D009693:Nucleic Acid Hybridization
chemical_list: D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein