A reagentless nitric oxide biosensor based on haemoglobin/polyethyleneimine film.
An unmediated NO biosensor was prepared by co-immobilizing haemoglobin with polyethyleneimine at a pyrolytic graphite electrode surface. Haemoglobin exhibits direct electrochemistry in the membrane, and this modified electrode shows high stability and catalytic activity towards the reduction of NO. A linear relationship between the catalytic current and the concentration of NO was observed in the concentration ranges 1.0 x 10(-7)-8.0 x 10(-6) M and 1.0 x 10(-5)-2.5 x 10(-5) M, with a detection limit of 4.0 x 10(-8) M.
Liu
Xinjian
X
Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, People's Republic of China.
Shang
Libin
L
Pang
Jiantao
J
Li
Genxi
G
D000818:Animals; D015374:Biosensing Techniques; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D004563:Electrochemistry; D004566:Electrodes; D006454:Hemoglobins / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009569:Nitric Oxide / Q000032:analysis*; D011094:Polyethyleneimine / Q000737:chemistry*
D006454:Hemoglobins; D009569:Nitric Oxide; D011094:Polyethyleneimine