title: Alpha1 and alpha2 domains of Aotus MHC class I and Catarrhini MHC class Ia share similar characteristics.
abstract: Functional and structural analyses of major histocompatibility complex (MHC) class I molecules of the Aotus genus are necessary to validate it as a solid animal model for biomedical research. We thus isolated, cloned and sequenced exons 2 and 3 from three Aotus species (A. nancymaae, A. nigriceps and A. vociferans). We found 24 sequences, which divided into two different groups (Ao-g1 and Ao-g2). A further sequence was identified as a processed pseudogene (Aona-PS2). Both sequence evolution and variability analyses showed that Ao-g1 and Ao-g2 display similar characteristics to Catarrhini's classical loci, such as positive selection pressure at the peptide binding region (PBR) high variability and a trans-specific evolution pattern.
authors.affiliation: Fundación Instituto de Inmunología de Colombia, FIDIC, Bogotá, Colombia. carlos_suarez@fidic.org.co
authors.forename: C F
authors.initials: CF
authors.lastname: Suárez
authors.forename: P P
authors.initials: PP
authors.lastname: Cárdenas
authors.forename: E J
authors.initials: EJ
authors.lastname: Llanos-Ballestas
authors.forename: P
authors.initials: P
authors.lastname: Martínez
authors.forename: M
authors.initials: M
authors.lastname: Obregón
authors.forename: M E
authors.initials: ME
authors.lastname: Patarroyo
authors.forename: M A
authors.initials: MA
authors.lastname: Patarroyo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016645:Aotidae / Q000235:genetics*; D002520:Cercopithecidae / Q000235:genetics*; D005260:Female; D005805:Genes, MHC Class I; D014644:Genetic Variation; D015395:Histocompatibility Antigens Class I / Q000145:classification / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid
chemical_list: D015395:Histocompatibility Antigens Class I; D010446:Peptide Fragments