title: [Influence of super high molecular weight poly D,L-lactic acid on viability and new bone formation of osteoblasts].
abstract: OBJECTIVE
  To investigate the influence of the viability and new bone formation of osteoblasts by the super high molecular weight poly D,L-lactic acid (SHMW-PDLLA).
  
  
  METHODS
  1. The osteoblasts derived from neonatal rat were grown and maintained at steep of SHMW-PDLLA and normal culture medium. The viability and function of the osteoblasts were measured with MTT array. 2. The plate and screws made of SHMW-PDLLA were implanted and fixed at the artificial fractured mandible of dogs. Specimens were gained at 3 and 6 months and examined with macroscopy and SEM.
  
  
  RESULTS
  1. There is no significant difference of OD values between the experimental group and the control group (P > 0.05). The SHMW-PDLLA isn't toxic to osteoblast at 1 week and 2 weeks, and the toxicity is 3% at 3 days. 2. There were a lot of new bone formed between the implanted SHMW-PDLLA plate and bone tissues under SEM.
  
  
  CONCLUSIONS
  SHMW-PDLLA hasn't pathological influence on the viability and new bone formation of osteoblasts and it is feasible in tissue engineering of bone.
authors.affiliation: Department of Oral and Maxillofacial Surgery, West China College of Stomatology, Sichuan University, Chengdu 610041, China.
authors.forename: Shi-cheng
authors.initials: SC
authors.lastname: Wei
authors.forename: Qian
authors.initials: Q
authors.lastname: Zheng
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Sheng-wei
authors.initials: SW
authors.lastname: Li
authors.forename: Han-zhang
authors.initials: HZ
authors.lastname: Wang
authors.forename: Cheng-dong
authors.initials: CD
authors.lastname: Xiong
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001860:Bone Plates; D001863:Bone Screws; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D004285:Dogs; D019344:Lactic Acid / Q000737:chemistry / Q000494:pharmacology*; D008337:Mandibular Fractures / Q000601:surgery; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D019647:Oral Surgical Procedures / Q000295:instrumentation / Q000379:methods; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D010012:Osteogenesis / Q000187:drug effects*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats
chemical_list: D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)