title: [EB virus infection and expression of P53 and P16 in non-Hodgkin's lymphoma].
abstract: OBJECTIVE
  To observe the presence of Epstin-Barr virus (EBV) and expression of P53 and P16 in the non-Hodgkin's lymphoma (NHL) occurring in the nasal cavity and pharyngeal Waldeyer's ring.
  
  
  METHODS
  In situ hybridization was used to detect EBV-DNA and P16-mRNA and immunohistochemical staining was used to examine the expression of LMP1, P53 and P16 in the 36 cases. Five cases whose EBV-DNA were negative at first assay by hybridization was re-detected for EBV-DNA by PCR.
  
  
  RESULTS
  Positive expression rates of EBV-DNA, LMP1, P16 mRNA, P53 and P16 were 63.9%, 27.8%, 30.6%, 19.4% and 22.2%. P53 and P16 expressions were not significantly different between T cell NHL and B cell NHL, nor were them significantly different between EBV positive and negative cases.
  
  
  CONCLUSIONS
  A higher percentage of EBV infection was detected in the nasal cavity and pharyngeal Waldeyer's ring in NHL. EBV infection was not significantly associated with P53 and P16 expresions in NHL.
authors.affiliation: Department of Pathology, Changhai Hospital, Second Military Medical University, Shanghai 200433.
authors.forename: L
authors.initials: L
authors.lastname: Wu
authors.forename: G
authors.initials: G
authors.lastname: Gu
authors.forename: D
authors.initials: D
authors.lastname: Ma
mesh_terms: D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000096:biosynthesis*; D020031:Epstein-Barr Virus Infections / Q000378:metabolism*; D006801:Humans; D017403:In Situ Hybridization; D008228:Lymphoma, Non-Hodgkin / Q000378:metabolism / Q000821:virology*; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism / Q000821:virology*; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis*
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16; D016159:Tumor Suppressor Protein p53