title: Environmental dispersal of Cryptosporidium parvum oocysts and cross transmission in cultured bivalve molluscs.
abstract: Two commercially valuable mollusc species ( Ostrea edulisand Tapes decussatus) were experimentally contaminated with Cryptosporidium parvum oocysts. A direct immunofluorescent antibody technique and inclusion/exclusion of the fluorogenic vital dye propidium iodide were used to test for the presence and viability of the oocysts, showing that transmission of contamination occurred between coexisting species. There was a decrease in the viability of oocysts in the initially uncontaminated molluscs as well as a large decrease in the number of oocysts retained when dead molluscs were used as the source of contamination. The results show the potentially important role that these molluscs play in spreading contamination in depuration plants and areas where aquatic organisms are cultivated.
authors.affiliation: Laboratorio de Parasitología, Departamento de Microbiología y Parasitología, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, A Coruña, Spain.
authors.forename: H
authors.initials: H
authors.lastname: Gómez-Couso
authors.forename: F
authors.initials: F
authors.lastname: Freire-Santos
authors.forename: M R
authors.initials: MR
authors.lastname: Ortega-Iñarrea
authors.forename: J A
authors.initials: JA
authors.lastname: Castro-Hermida
authors.forename: M E
authors.initials: ME
authors.lastname: Ares-Mazás
mesh_terms: D000818:Animals; D003457:Cryptosporidiosis / Q000635:transmission; D016785:Cryptosporidium parvum / Q000254:growth & development / Q000302:isolation & purification* / Q000472:pathogenicity; D003470:Culture Media; D004783:Environmental Microbiology; D006790:Host-Parasite Interactions; D008974:Mollusca / Q000469:parasitology*; D033661:Oocysts / Q000302:isolation & purification; D010270:Parasite Egg Count; D014871:Water Microbiology
chemical_list: D003470:Culture Media