Automated pneumococcal MLST using liquid-handling robotics and a capillary DNA sequencer.
Multilocus sequence typing (MLST) is used by the Scottish Meningococcus and Pneumococcus Reference Laboratory (SMPRL) as a routine method for the characterization of certain bacterial pathogens. The SMPRL recently started performing MLST on strains of Streptococcus pneumoniae, and here we describe a fully automated method for MLST using a 96-well-format liquid-handling robot and a 96-capillary automated DNA sequencer.
Jefferies
Johanna
J
Scottish Meningococcus and Pneumococcus Reference Laboratory, North Glasgow University Hospital NHS Trust, Department of Microbiology, Stobhill Hospital, Balornock Rd., Glasgow, G21 3UW, UK.
Clarke
Stuart C
SC
Diggle
Mathew A
MA
Smith
Andrew
A
Dowson
Chris
C
Mitchell
Tim
T
D001331:Automation; D015373:Bacterial Typing Techniques / Q000379:methods*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods; D013296:Streptococcus pneumoniae / Q000235:genetics*
D004269:DNA, Bacterial