title: [HPLC determination of two flavonoid compounds in Psoralea corylifolia].
abstract: OBJECTIVE
  To determine two flavonoid compounds in Psoralea corylifolia L. (PC) simultaneously with HPLC method.
  
  
  METHODS
  Bavachin and corylin isolated from PC and purified in our laboratory were used as the reference compounds. The HPLC separation was carried out on an Techsphere ODS column using mobile phase consisting of a mixture of methanol and 20 mmol.L-1 ammonium acetate buffer pH 4.0 (67:33), and the UV detection wavelength was 240 nm.
  
  
  RESULTS
  Simultaneous determination of bavachin and corylin was achieved. The linear range was 1.25-20 micrograms.mL-1 for both bavachin and corylin. The average recovery of bavachin and corylin was 94.9% and 96.2%, and RSD was 3.1% and 3.6% respectively.
  
  
  CONCLUSIONS
  This is the first report on simultaneous determination of bavachin and corylin in PC with satisfactory accuracy and reproducibility.
authors.affiliation: Department of Chemistry, Anshan Teacher's College, Anshan 114005, Liaoning, China.
authors.forename: Rui-min
authors.initials: RM
authors.lastname: Lin
authors.forename: Da-wei
authors.initials: DW
authors.lastname: Wang
authors.forename: Zhi-li
authors.initials: ZL
authors.lastname: Xiong
authors.forename: Yong
authors.initials: Y
authors.lastname: Xu
authors.forename: Fa-mei
authors.initials: FM
authors.lastname: Li
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis*; D005638:Fruit / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D029907:Psoralea / Q000737:chemistry*
chemical_list: D005419:Flavonoids; C459212:bavachin; C459211:corylin