Cloning the full-length cDNA of a gene responsible for vascular smooth muscle cell proliferation in atherogenesis and study of function.
OBJECTIVE
To clone the full-length cDNA of a gene responsible for vascular smooth muscle cell (v-SMC) proliferation in atherogenesis, and study its function.


METHODS
Oxidized low density lipoprotein (ox-LDL) at optimal concentration was used as the stimulant to induce v-SMC proliferation in culture medium. A cDNA subtractive library of v-SMC proliferation specific to ox-LDL stimulation was established using subtractive hybridization technique. Methods, including blotting, Northern hybridization and gene sequencing, were used to clone new gene fragments. By using full-length cDNA screening and protein expression techniques, one full-length cDNA was cloned and its function was studied.


RESULTS
One full-length cDNA was cloned. The new gene (Genbank AF 174647) expressed a 44 kDa protein, which might be associated with the activity of ox-LDL.


CONCLUSIONS
The new gene cloned may be associated with SMC proliferation in atherogenesis.
Zhao
Gaofeng
G
Department of Chest Surgery, The First Hospital of Henan Medical University, Zhengzhou 450052, China.
Gao
Peng
P
Zhang
Hua
H
Lou
Liming
L
Qiao
Huihong
H
She
Mingpeng
M
D000595:Amino Acid Sequence; D001161:Arteriosclerosis / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003001:Cloning, Molecular; D015723:Gene Library; D006801:Humans; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000187:drug effects*; D009693:Nucleic Acid Hybridization
D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein