The VirR/VirS regulatory cascade affects transcription of plasmid-encoded putative virulence genes in Clostridium perfringens strain 13.
We analyzed the transcriptional regulation of the putative virulence genes encoded on the plasmid pCP13 from Clostridium perfringens strain 13. The transcription of the beta2-toxin (cpb2) and possible collagen adhesin (cna) genes were regulated in both a positive and negative manner, respectively, by the two-component VirR/VirS system. The secondary regulator of the VirR/VirS system, VR-RNA, also affects the expression of both of these genes in the same fashion as the VirR/VirS system. This indicates that the global regulatory cascade of the VirR/VirS system controls the expression of virulence genes located on the plasmid, as well as those found chromosomally in C. perfringens strain 13.
Ohtani
Kaori
K
Department of Infection Biology, Institute of Basic Medical Sciences, University of Tsukuba, 1-1-1, Tennohdai, Ibaraki Tsukuba 305-8575, Japan.
Kawsar
Hameem I
HI
Okumura
Kayo
K
Hayashi
Hideo
H
Shimizu
Tohru
T
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D001427:Bacterial Toxins / Q000235:genetics; D001483:Base Sequence; D003016:Clostridium perfringens / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D014158:Transcription, Genetic; D014774:Virulence
D001426:Bacterial Proteins; D001427:Bacterial Toxins; C075216:Clostridium novyi alpha-toxin; D012329:RNA, Bacterial; C109896:cpb2 protein, Clostridium perfringens; C068267:mry protein, Streptococcus pyogenes