Effect of transportation and commingling on the acute-phase protein response, growth, and feed intake of newly weaned beef calves.
The objective of this study was to investigate the effect of transportation and commingling on measures of the acute-phase protein response in newly weaned beef calves. Thirty-two (Exp. 1; average BW = 266 +/- 20.8 kg) and thirty-six (Exp. 2; average BW = 222 +/- 34.6 kg) Brahman-crossbred calves were randomly allotted to one of four treatments (2 x 2 factorial arrangement [transportation x commingling] in a completely randomized design). Body weight and jugular blood were collected at weaning, after shipment, and 1, 3, and 7 d after transport for Exp. 1, and at weaning and 1, 5, 9, 13, 17, and 21 d after transport for Exp. 2. Feed intake within pen was recorded daily for Exp. 2. Plasma fibrinogen, ceruloplasmin, haptoglobin, and cortisol concentrations were determined for all collection times. Additionally, serum amyloid-A and alpha-acid glycoprotein concentrations were determined in Exp. 1 and 2, respectively. In Exp. 2, commingled calves tended (P = 0.13) to have a higher DMI than noncommingled calves (5.3 and 4.8 kg/d, respectively). Transported calves lost more BW than nontransported calves from the time of weaning to d 1 (2.0 and 3.1% more BW loss for Exp. 1 and 2, respectively). With the exception of haptoglobin in Exp. 1, each of the acute-phase proteins measured in these studies increased over each sampling day. In Exp. 1, transported calves had higher (P < 0.05) mean serum amyloid-A concentrations than nontransported calves (48.9 vs. 33.4 microg/mL). There was a significant sampling day x transportation interaction (P < 0.01) for fibrinogen, ceruloplasmin, and haptoglobin in Exp. 1; transported calves had higher concentrations of fibrinogen following transport and on d 2 and 3, and ceruloplasmin on d 3. Haptoglobin concentrations were higher (P = 0.04) in nontransported calves on d 1 and 2 of Exp. 1. In Exp. 2, overall mean haptoglobin concentrations were higher in nontransported vs. transported calves. The results of these studies indicate that stressors associated with transportation affect the acute-phase protein response in newly weaned beef calves. More research is needed to determine whether these proteins might be valuable indicators of stress following the weaning process.
Arthington
J D
JD
University of Florida-IFAS, Range Cattle Research and Education Center, Ona, USA. jdarthington@mail.ifas.ufl.edu
Eichert
S D
SD
Kunkle
W E
WE
Martin
F G
FG
D000209:Acute-Phase Proteins / Q000378:metabolism*; D000822:Animal Husbandry / Q000379:methods; D000818:Animals; D015415:Biomarkers / Q000097:blood; D002417:Cattle / Q000097:blood* / Q000254:growth & development / Q000502:physiology / Q000523:psychology; D002149:Energy Intake; D005260:Female; D006023:Glycoproteins / Q000097:blood; D006854:Hydrocortisone / Q000097:blood; D008297:Male; D011897:Random Allocation; D000685:Serum Amyloid A Protein / Q000378:metabolism; D013315:Stress, Psychological / Q000097:blood / Q000378:metabolism*; D013997:Time Factors; D014186:Transportation; D014886:Weaning; D015430:Weight Gain
D000209:Acute-Phase Proteins; D015415:Biomarkers; D006023:Glycoproteins; D000685:Serum Amyloid A Protein; D006854:Hydrocortisone