title: Determination of cyclonite (RDX) in human plasma by high-performance liquid chromatography.
abstract: A simple and sensitive HPLC method has been developed for the assay of cyclonite (RDX) in human plasma. The assay involves solid-phase extraction on Tox-clean RC SPE cartridges and isocratic reversed-phase chromatography with diode-array detection. The assay was linear over the concentration range of 0.01-2.0 microg/ml for plasma with a lower limit of detection of 0.005 microg/ml. Both the within-day and day-to-day reproducibilities and accuracies were less than 10.15 and 1.9%, respectively. The method was applied to evaluate RDX concentration in plasma samples obtained from soldiers exposed RDX.
authors.affiliation: Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Istanbul University, 34116 Istanbul, Turkey.
authors.forename: Gül
authors.initials: G
authors.lastname: Ozhan
authors.forename: Sibel
authors.initials: S
authors.lastname: Topuz
authors.forename: Buket
authors.initials: B
authors.lastname: Alpertunga
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D010949:Plasma / Q000737:chemistry*; D014227:Triazines / Q000097:blood*
chemical_list: D014227:Triazines; C009160:cyclonite