[Study of protective effect of leech, radix Salviae miltiorrhizae and its composite recipe on vascular endothelial cells in rats with blood stasis syndrome].
OBJECTIVE
To study the protective effect for Chinese medicine of promoting blood circulation and removing blood stasis (PBCRBS) on vascular endothelial cells (VEC) in rats, aiming at further research on the mechanism of blood stasis syndrome and PBCRBS.


METHODS
Establishing a model of blood stasis with endothelial damage by means of giving rats an injection of adrenalin and making it swim in ice-cold water, then measuring the number of circulating endothelial cells (CEC) in whole blood and rheology. Moreover observing the change of the above indexes after the rats have taken Leech, Radix salviae miltrorrhizae (RSM) and its composite recipe for 5 days or 10 days.


RESULTS
It could increase the number of CEC accompanied by whole blood viscosity, fibrinogen and hematocrit in rats with blood stasis. But for groups taking Chinese medicine, the number of CEC was decreased distinctively and blood rheology was improved.


CONCLUSIONS
The rat with blood stasis syndrome suffered from obvious injury of VEC. The Leech, RSM and its composite recipe could protect VEC.
Li
F W
FW
Institute of Basic Theory, China Academy of Traditinal Chinese Medicine, Beijing 100700, China.
Zhang
L S
LS
Liu
H
H
Cai
Y C
YC
Pan
J H
JH
Jia
X Y
XY
Ding
J X
JX
D000818:Animals; D002452:Cell Count; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D005260:Female; D018056:Hemorheology / Q000187:drug effects; D007865:Leeches / Q000737:chemistry; D008297:Male; D008419:Materia Medica / Q000302:isolation & purification / Q000494:pharmacology; D008516:Medicine, Chinese Traditional; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D027561:Salvia miltiorrhiza / Q000737:chemistry
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D008419:Materia Medica