title: Phospholipase activity on N-acyl phosphatidylethanolamines is critically dependent on the N-acyl chain length.
abstract: We have recently shown that an endogenous phospholipase A2 from bovine erythrocytes does not hydrolyse NAPEs (N-acyl L-alpha-phosphatidylethanolamines), which accumulate remarkably in this system [Florin-Christensen, Suarez, Florin-Christensen, Wainszelbaum, Brown, McElwain and Palmer (2001) Proc. Natl. Acad. Sci. U.S.A. 98, 7736-7741]. Here we investigate the causes underlying this resistance. N-acylation of PE (L-alpha-phosphatidylethanolamine) results in alteration of charge, head-group volume and conformation, the last two features depending on the N-acyl chain length. To evaluate each effect separately, we synthesized NAPEs with selected N-acyl chain length. We found that phospholipase A2 has considerable activity against N-acetyl PE, but is poorly active against N-butanoyl PE and only marginally active against N-hexanoyl PE, whereas the activity is completely lost when N-hexadecanoyl PE is presented as a substrate. On the other hand, N-hexanoyl PE does not inhibit phospholipase A2 activity, suggesting that this substrate fails to enter the hydrophobic channel. Phospholipase C presents a similar, but less sharp pattern. Molecular dynamics simulations of the polar head group of selected NAPEs reveal a substantially increased conformational variability as the N-acyl chain grows. This larger conformational space represents an increased impairment limiting the access of these molecules to the active site. Our data indicate that, whereas a change in charge contributes to diminished activity, the most relevant effects come from steric hindrance related to the growth of the N-acyl chain.
authors.affiliation: Institute of Biophysics and Biochemistry (IQUIFIB, UBA-CONICET), School of Pharmacy and Biochemistry, University of Buenos Aires, Junín 956, RA-1113, Buenos Aires, Argentina.
authors.forename: Julio J
authors.initials: JJ
authors.lastname: Caramelo
authors.forename: Jorge
authors.initials: J
authors.lastname: Florin-Christensen
authors.forename: José M
authors.initials: JM
authors.lastname: Delfino
mesh_terms: D000215:Acylation; D000818:Animals; D001409:Bacillus cereus / Q000201:enzymology; D001665:Binding Sites; D017815:Elapidae; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D010740:Phospholipases / Q000737:chemistry* / Q000378:metabolism*; D011487:Protein Conformation; D013379:Substrate Specificity
chemical_list: D010714:Phosphatidylethanolamines; D010740:Phospholipases