Accidental infection of laboratory worker with vaccinia virus.
We report the accidental needlestick inoculation of a laboratory worker with vaccinia virus. Although the patient had previously been vaccinated against smallpox, severe lesions appeared on the fingers. Western blot and polymerase chain reaction-restriction fragment length polymorphism were used to analyze the virus recovered from the lesions. The vaccinia virus-specific immunoglobulin G levels were measured by enzyme-linked immunosorbent assay. Our study supports the need for vaccination for laboratory workers that routinely handle orthopoxvirus.
Moussatché
Nissin
N
Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro Brazil. nissin@biof.ufrj.br
Tuyama
Mari
M
Kato
Sayuri E M
SE
Castro
Ana Paula V
AP
Njaine
Brian
B
Peralta
Regina H
RH
Peralta
José M
JM
Damaso
Clarissa R A
CR
Barroso
Paulo F
PF
D000328:Adult; D000914:Antibodies, Viral / Q000097:blood; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007757:Laboratory Infection / Q000175:diagnosis / Q000209:etiology* / Q000821:virology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D014615:Vaccinia / Q000175:diagnosis / Q000209:etiology*; D014616:Vaccinia virus / Q000302:isolation & purification
D000914:Antibodies, Viral; D004279:DNA, Viral; D007074:Immunoglobulin G