[Effects of nourishing shen-yin recipe and warming recuperating shen-yang recipe on interleukin-1 and interleukin-6 activity in ovariectomy induced osteoporosis rats].
OBJECTIVE
To compare the effects of the recipes for nourishing Shen-yin (NSY) and for warming recuperating Shen-yang (WRSY) on the activity of interleukin-1 (IL-1) and interleukin-6 (IL-6) in ovariectomy-induced osteoporosis rats.


METHODS
Fifty-five female rats were randomly divided into five groups: the control, the sham operation, the ovariectomy, the ovariectomy plus NSY and the ovariectomy plus WRSY group. The medication was starting from one week after operation and lasted continuously for 3 months. The proximal tibia of rats were analyzed with bone histomorphometry, the activity of IL-1 was detected with mouse thymocyte assay and the activity of IL-6 was detected with IL-6 dependence cell proliferation assay.


RESULTS
Three months after ovariectomy, the percentage of trabeculae bone volume (TBV%) of tibia in rats was markedly reduced, but the percentage of trabeculae absorption surface (TRS%) and trabeculae formation surface (TFS%) significantly increased, and the activity of IL-1 and IL-6 increased obviously. These abnormal changes could be reversed to different degree by NSY or WRSY, but the effect of the latter was superior to that of the former.


CONCLUSIONS
The inhibitory effect of WRSY on IL-1 and IL-6 activity was significantly better than that of NSY, which is one of the mechanisms of its superiority in preventing osteoporosis induced by ovariectomy in rats.
Ju
Da-hong
DH
Institute of Basic Theory, China Academy of Traditional Chinese Medicine, Beijing 100700. Judahong@Sohu.com
Lu
Ai-ping
AP
Zhang
Chun-ying
CY
D000818:Animals; D004359:Drug Therapy, Combination; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D007375:Interleukin-1 / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism*; D010024:Osteoporosis / Q000209:etiology / Q000378:metabolism*; D010052:Ovariectomy; D008517:Phytotherapy; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D004365:Drugs, Chinese Herbal; D007375:Interleukin-1; D015850:Interleukin-6