A microtiter-plate screening method for biofilm disinfection and removal.
A quantitative spectrophotometric method was developed to measure the removal and killing efficacy of antibiofilm agents. Biofilms of Pseudomonas aeruginosa or Staphylococcus epidermidis were grown in 96-well plates, treated with an agent, then stained with either the biomass indicator crystal violet or the respiratory indicator 5-cyano-2,3-ditolyl tetrazolium chloride. This rapid screening method is sensitive enough to elucidate concentration-response relationships as well as differences between species responses to treatments. Using these assays, agents can be ranked by their ability to remove or kill biofilm.
Pitts
Betsey
B
Center for Biofilm Engineering, Montana State University, Bozeman, MT 59717, USA.
Hamilton
Martin A
MA
Zelver
Nicholas
N
Stewart
Philip S
PS
D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D015169:Colony Count, Microbial; D004202:Disinfectants / Q000008:administration & dosage; D004203:Disinfection; D004305:Dose-Response Relationship, Drug; D008826:Microbial Sensitivity Tests / Q000379:methods*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development; D012973:Sodium Hypochlorite / Q000494:pharmacology; D013053:Spectrophotometry; D013194:Staining and Labeling / Q000379:methods; D013212:Staphylococcus epidermidis / Q000187:drug effects* / Q000254:growth & development; D013778:Tetrazolium Salts / Q000737:chemistry; D014508:Urea / Q000494:pharmacology
D004202:Disinfectants; D013778:Tetrazolium Salts; C075453:5-cyano-2,3-ditolyltetrazolium; D014508:Urea; D012973:Sodium Hypochlorite