title: Preparation of vitamin K-dependent proteins, such as clotting factors II, VII, IX and X and clotting inhibitor protein C.
abstract: A review is given of preparative methods for the isolation of the vitamin K-dependent clotting factors II, VII, IX, X and clotting inhibitor protein C, all derived from human plasma. Factor II, activated factor VII and activated protein C are also obtained from recombinant animal cells. The methods for their purification are described. The problem of difference in posttranslational modifications between plasma derived and recombinant protein is discussed with regard to therapeutic proteins.
authors.affiliation: Octapharma Pharmazeutika Produktions Ges.m.b.H., Oberlaaerstrasse 235, A-1100, Vienna, Austria. djuro.josic@octapharma.at
authors.forename: Djuro
authors.initials: D
authors.lastname: Josic
authors.forename: Lutz
authors.initials: L
authors.lastname: Hoffer
authors.forename: Andrea
authors.initials: A
authors.lastname: Buchacher
mesh_terms: D000818:Animals; D001779:Blood Coagulation Factors / Q000737:chemistry* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D011486:Protein C / Q000737:chemistry* / Q000302:isolation & purification; D014812:Vitamin K / Q000494:pharmacology*
chemical_list: D001779:Blood Coagulation Factors; D011486:Protein C; D014812:Vitamin K