Acute lead exposure induces renal haeme oxygenase-1 and decreases urinary Na+ excretion.
The effects of acute lead exposure on renal function, lipid peroxidation and the expression of haeme oxygenase (HO) in rat kidney were determined. A single injection of lead acetate (50 mg Pb/kg) was given to rats. Changes in renal function, characterized by a significant reduction in the Na+ excretion was observed six hours after Pb exposure; this effect persisted for 24 hours. TBARS levels increased in kidney cortex 24 hours after Pb administration. In kidney cortex, Pb exposure affected the expression of HO-1, a renal protein associated with oxidative stress. HO-1 mRNA increased 2.3-fold, three hours after Pb administration and remained increased for six, 12 and 24 hours. HO enzymatic activity and HO-1 protein increased six and three hours after Pb administration, respectively, and remained increased at 24 hours. HO inhibition by tin-protoporphyrin, potentiated Pb-induced increase in TBARS and prevented the Pb-induced reduction in Na+ excretion. Our data suggest that Pb may be acting through the generation of oxidant products and induction of HO.
Vargas
Hilda
H
Escuela Superior de Medicina Instituto Politécnico Nacional México D.F., México. bescalan@mail.cinvestav.mx
Castillo
Carlos
C
Posadas
Francisco
F
Escalante
Bruno
B
D015870:Gene Expression / Q000187:drug effects / Q000235:genetics; D006419:Heme Oxygenase (Decyclizing) / Q000096:biosynthesis* / Q000187:drug effects* / Q000235:genetics; D007672:Kidney Cortex / Q000187:drug effects* / Q000378:metabolism* / Q000503:physiopathology; D007854:Lead / Q000008:administration & dosage / Q000097:blood / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D012964:Sodium / Q000652:urine*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014556:Urine / Q000737:chemistry / Q000502:physiology*
D012333:RNA, Messenger; D017392:Thiobarbituric Acid Reactive Substances; D007854:Lead; D012964:Sodium; D006419:Heme Oxygenase (Decyclizing)