title: Preservation of yeast cell morphology for scanning electron microscopy using 3.28-micro m IR laser irradiation.
abstract: As an alternative to conventional fixation procedures for scanning electron microscopy (SEM) analysis, yeast cells (Saccharomyces cerevisiae) were irradiated in ambient air, with an intense 3.28-micro m IR laser pulse. The morphology of the irradiated cells was well preserved, while nonirradiated control cells were severely shriveled.
authors.affiliation: Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59717, USA. sunner@chemistry.montana.edu
authors.forename: Jan
authors.initials: J
authors.lastname: Sunner
authors.forename: Recep
authors.initials: R
authors.lastname: Avci
authors.forename: Lee
authors.initials: L
authors.lastname: Richards
authors.forename: Gary
authors.initials: G
authors.lastname: Groenewold
authors.forename: Jani
authors.initials: J
authors.lastname: Ingram
authors.forename: Megan
authors.initials: M
authors.lastname: Arthun
mesh_terms: D006046:Gold / Q000737:chemistry; D007834:Lasers; D008855:Microscopy, Electron, Scanning / Q000379:methods; D011309:Preservation, Biological; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000528:radiation effects / Q000648:ultrastructure*
chemical_list: D006046:Gold