title: Comparative study of the N-terminal hydrophilic region in Streptomyces lividans and E. coli FtsY.
abstract: Streptomyces lividans FtsY (SlFtsY) was cloned and overexpressed in Escherichia coli. Analysis of the amino acid (aa) sequence showed a concentration of hydrophilic aa's in the N-terminal half region of SlFtsY as observed in that of E. coli FtsY (EcFtsY). However, the length of the hydrophilic region was shorter in SlFtsY than in EcFtsY. Overexpression of SlFtsY in E. coli resulted in growth suppression as in the case of the overexpression of EcFtsY, while growth suppression as a result of the overexpression of the C-terminal half region of SlFtsY was limited. This result suggests that the N-terminal hydrophilic region of SlFtsY, regardless of its short length, would behave like its counterpart region of EcFtsY in E. coli.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Isamu
authors.initials: I
authors.lastname: Maeda
authors.forename: Nozomi
authors.initials: N
authors.lastname: Hirose
authors.forename: Hidenori
authors.initials: H
authors.lastname: Yamashiro
authors.forename: Ayako
authors.initials: A
authors.lastname: Ichibori
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Ohshima
authors.forename: Tomonori
authors.initials: T
authors.lastname: Fujimoto
authors.forename: Masaya
authors.initials: M
authors.lastname: Kawase
authors.forename: Kiyohito
authors.initials: K
authors.lastname: Yagi
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D015870:Gene Expression; D008969:Molecular Sequence Data; D018160:Receptors, Cytoplasmic and Nuclear / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D013302:Streptomyces / Q000737:chemistry / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; C087406:FtsY protein, Bacteria; D018160:Receptors, Cytoplasmic and Nuclear; D011994:Recombinant Proteins