title: In vitro antibacterial activity of a 7-O-beta-D-glucopyranosyl-nutanocoumarin from Chaptalia nutans (Asteraceae).
abstract: Ethanolic crude extracts from the roots of Chaptalia nutans, traditionally used in Brazilian folk medicine, were screened against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa by using the disk diffusion test technique. S. aureus with 14 mm inhibition zone was considered susceptible. E. coli and P. aeruginosa without such a zone were considered resistant. As a result of this finding, the ethanolic crude extract was fractionated on silica gel column chromatography into five fractions. The ethyl acetate fraction was active against S. aureus and Bacillus subtilis. Further column chromatography separation of the ethyl acetate fraction afforded 30 fractions, which were assayed against S. aureus. Fractions 16 and 17 showed inhibition zones with S. aureus, indicating the presence of active compounds, and were subjected to purification by repeated preparative thin layer chromatography. The pure compound 7-O-beta-D-glucopyranosyl-nutanocoumarin inhibited B. subtilis and S. aureus at concentrations of 62.5  g/ml and 125  g/ml, respectively. The antibacterial property of C. nutans appears to have justified its use for the treatment of wounds, which are contaminated through bacterial infections.
authors.affiliation: Departamento de Farmácia e Farmacologia, Universidade Estadual de Maringá, Maringá, PR, Brasil.
authors.forename: Maria da Conceião Torrado
authors.initials: Mda C
authors.lastname: Truiti
authors.forename: Maria Helena
authors.initials: MH
authors.lastname: Sarragiotto
authors.forename: Benício Alves
authors.initials: BA
authors.lastname: de Abreu Filho
authors.forename: Celso Vataru
authors.initials: CV
authors.lastname: Nakamura
authors.forename: Benedito Prado
authors.initials: BP
authors.lastname: Dias Filho
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D019659:Asteraceae; D003374:Coumarins / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects*; D005960:Glucosides / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology; D010946:Plants, Medicinal; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D013211:Staphylococcus aureus / Q000187:drug effects*
chemical_list: C475604:7-O-beta-D-glucopyranosyl-nutanocoumarin; D000900:Anti-Bacterial Agents; D003374:Coumarins; D005960:Glucosides; D010936:Plant Extracts