[Expression of apoptotic gene and FGF on olfactory bulb of rat and its significance].
OBJECTIVE
To study the expression and distribution of apoptotic gene Bcl-2 and Bax on olfactory of rat, and its relation with the FGF and to investigate the mechanism of senile dysosmia.


METHODS
Ten young (3 months) and ten senile rats (24 months) were used in this study. After the removal of the heads of these rats, olfactory bulb were immediately fixed with neutralized formalin, followed with paraffin-embedding, serial sectioning, immunohistochemiscal staining and microscopic observing.


RESULTS
The positive expression rate of Bcl-2 and FGF on olfactory bulb in young rats was significantly stronger than that in senile rats (P < 0.01). The positive expression rate of Bax in senile rats was a little stronger than that in young rats, but the differences were not statistically significant. (P > 0.05)


CONCLUSIONS
The expression of FGF and anti-apoptotic gene Bcl-2 on olfactory bulb decrease with the increase of age. This suggests neuron apoptosis on olfactory bulb are regulated by neurotrophic factor, and directly related with senile dysosmia. The mitral cell apoptosis as a result of FGF decreased on olfactory bulb may play a key role in the senile dysosmia.
Guan
G
G
Department of Otorhinolaryngology, Third College of Norman Bethune, University of Medical Sciences, Changchun 130031, China. wangyipe@public.cc.jl.cn
Dong
Z
Z
Yang
Z
Z
D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D005346:Fibroblast Growth Factors / Q000096:biosynthesis*; D019254:Genes, bcl-2; D008297:Male; D000857:Olfaction Disorders / Q000378:metabolism; D009830:Olfactory Bulb / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000235:genetics; D019253:Proto-Oncogene Proteins c-bcl-2; D051381:Rats; D051028:bcl-2-Associated X Protein
C494828:Bax protein, rat; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D005346:Fibroblast Growth Factors