title: Molecular characterization of a defensin in the IZD-MB-0503 cell line derived from immunocytes of the insect Mamestra brassicae (Lepidoptera).
abstract: The induction of anti-microbial peptides against Gram positive and negative bacteria in the IZD-MB-0503 cell line from the lepidopteran Mamestra brassicae is demonstrated, while no anti-fungal activity is detected. The identification of a defensin-like molecule active against Gram positive bacteria is described for the first time in Lepidoptera. This molecule shows between 43% and 59% homology with group A defensins from other dipteran and hymenopteran species.
authors.affiliation: Department of Animal Biology, via Campi 213/D, 41100 Modena, Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: Mandrioli
authors.forename: Silvia
authors.initials: S
authors.lastname: Bugli
authors.forename: Sabrina
authors.initials: S
authors.lastname: Saltini
authors.forename: Susanna
authors.initials: S
authors.lastname: Genedani
authors.forename: Enzo
authors.initials: E
authors.lastname: Ottaviani
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D023082:Defensins / Q000235:genetics* / Q000276:immunology* / Q000494:pharmacology; D017344:Genes, Insect; D007915:Lepidoptera / Q000276:immunology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D023082:Defensins