title: B-cell marker negative (CD7+, CD19-) Epstein-Barr virus-related pyothorax-associated lymphoma with rearrangement in the JH gene.
abstract: Pyothorax-associated lymphoma (PAL) develops decades after receiving artificial pneumothorax for pulmonary tuberculosis. The lymphomas, develop in tissue affected by long-standing severe inflammatory process. Most cases demonstrate diffuse large B-cell lymphoma. We present a patient with T-cell phenotype-positive and B-cell phenotype-negative (CD7+, CD43+, CD19-, and CD20-) PAL. Southern blot hybridization using immunglobulin heavy chain J region (IgH) gene probe revealed a monoclonal rearrangement, and hybridization using T-cell receptor beta chain (TCR) gene probe revealed a germline configuration. This indicates that the tumor origin was of B-lymphocytes. Chromosomal abnormality of the lymphoma was complicated. It suggested that many transformations occurred. In the transformation process, probably B-cell antigens were lost, and T-cell antigens were aberrantly expressed.
authors.affiliation: Department of Hematology, Juntendo University School of Medicine, 2-1-1, Hongo Bunkyo-ku, Tokyo, 113-8421 Japan.
authors.forename: Shigehisa
authors.initials: S
authors.lastname: Tomita
authors.forename: Kiyoshi L
authors.initials: KL
authors.lastname: Mori
authors.forename: Sakura
authors.initials: S
authors.lastname: Sakajiri
authors.forename: Kazuo
authors.initials: K
authors.lastname: Oshimi
mesh_terms: D000368:Aged; D000911:Antibodies, Monoclonal / Q000378:metabolism; D015703:Antigens, CD; D018941:Antigens, CD19 / Q000096:biosynthesis*; D018951:Antigens, CD20 / Q000096:biosynthesis; D019011:Antigens, CD7 / Q000096:biosynthesis*; D001402:B-Lymphocytes / Q000378:metabolism*; D014408:Biomarkers, Tumor; D015139:Blotting, Southern; D002869:Chromosome Aberrations; D016724:Empyema, Pleural / Q000378:metabolism*; D004854:Herpesvirus 4, Human / Q000378:metabolism*; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D051917:Leukosialin; D008213:Lymphocyte Activation; D008223:Lymphoma / Q000821:virology*; D008297:Male; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D012795:Sialoglycoproteins / Q000096:biosynthesis; D013601:T-Lymphocytes / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018941:Antigens, CD19; D018951:Antigens, CD20; D019011:Antigens, CD7; D014408:Biomarkers, Tumor; D007136:Immunoglobulins; D051917:Leukosialin; C465014:SPN protein, human; D012795:Sialoglycoproteins