[Protective effect of adenoviral-mediated brain derived neurotrophic factor gene on spiral ganglion].
OBJECTIVE
To observe the expression of brain derived neurotrophic factor (BDNF) in the cochlea of guinea pig, and assess the activity of BDNF in spiral ganglion cell following the damage of noise.


METHODS
Twenty-seven guinea pigs were exposed to a 4 kHz narrow band noise at 135 dB SPL for 4 hours. At seven days after the noise exposure, twelve guinea pigs were inoculated with ad-BDNF, and twelve guinea pigs were inoculated with ad-LacZ, while three guinea pigs were inoculated with artificial perilymphatic fluid. The animals were sacrificed After 1 week, 4 weeks and 8 weeks, respectively. The cochlea was stained with immunohistochemisty (ABC method) to examine the expression of BDNF. The number of spiral ganglion cell was counted to assess the activity of BDNF.


RESULTS
BDNF gene was expressed in whole cochlea, and the expression was obviously elevated 1 week after the adenovirus administration and reduced thereafter. The number of degenerated cells in ad-BDNF group in the eight weeks was lower than that in another two groups and the different between them was statistical significant (P < 0.01).


CONCLUSIONS
Adenoviral-mediated brain derived neurotrophic factor can be expressed in a high level in the cochlea of guinea pig, and may prevent the cell of spiral ganglion from the damage of noise. This study lays the groundwork for alleviation of hearing loss using gene therapy.
Zhai
Suoqiang
S
Institute of Otorhinolaryngology, Chinese PLA General Hospital, Beijing 100853, China. zhaisq@plagh.com.cn
Guo
Wei
W
Hu
Yinyan
Y
Chen
Qian
Q
Wang
Jiazheng
J
D000256:Adenoviridae / Q000502:physiology; D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis* / Q000235:genetics; D003051:Cochlea / Q000378:metabolism*; D015316:Genetic Therapy; D006168:Guinea Pigs; D006317:Hearing Loss, Noise-Induced / Q000628:therapy*; D013136:Spiral Ganglion / Q000473:pathology*
D019208:Brain-Derived Neurotrophic Factor