title: [Expressions of angiotensin II type 1 receptor mRNA in fibrotic and normal liver tissues].
abstract: OBJECTIVE
  To investigate the expression profile of angiotensin    type 1 receptor (AT1R) mRNA in fibrotic and normal liver tissues.
  
  
  METHODS
  Semi-quantitative RT-PCR was used to detect the expression of AT1R mRNA in 18 specimens of fibrotic liver tissues adjacent to liver cancers and 12 normal liver tissue specimens. Specific AT1R product and GAPDH product used as internal control were both amplified by RT-PCR, and the ratio of their integrated optical densities calculated to estimate the relative quantity of AT1R mRNA expression.
  
  
  RESULTS
  The relative mRNA expression of AT1R in fibrotic liver tissues was significantly higher than that of normal liver tissues (1.27+/-0.45 vs 0.71+/-0.21, P < 0.01).
  
  
  CONCLUSIONS
  AT1R may play an important role in the development of hepatic fibrosis.
authors.affiliation: Institute for Digestive Diseases of PLA, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. niehaibo36@hotmail.com
authors.forename: Wei-wei
authors.initials: WW
authors.lastname: Wang
authors.forename: Xi-shan
authors.initials: XS
authors.lastname: Yang
authors.forename: Ping-sheng
authors.initials: PS
authors.lastname: Wu
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Hong-bin
authors.initials: HB
authors.lastname: Zhang
authors.forename: Xu
authors.initials: X
authors.lastname: Li
authors.forename: Wen-ling
authors.initials: WL
authors.lastname: Zheng
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008103:Liver Cirrhosis / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis*; D044139:Receptor, Angiotensin, Type 2 / Q000235:genetics*; D012084:Renin-Angiotensin System / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger; D044139:Receptor, Angiotensin, Type 2