The glycosyltransferase gene encoding the enzyme catalyzing the first step of mycothiol biosynthesis (mshA).
Mycothiol is the major thiol present in most actinomycetes and is produced from the pseudodisaccharide 1D-myo-inosityl 2-acetamido-2-deoxy-alpha-D-glucopyranoside (GlcNAc-Ins). A transposon mutant of Mycobacterium smegmatis shown to be GlcNAc-Ins and mycothiol deficient was sequenced to identify a putative glycosyltransferase gene designated mshA. The ortholog in Mycobacterium tuberculosis, Rv0486, was used to complement the mutant phenotype.
Newton
Gerald L
GL
Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093, USA.
Koledin
Teresa
T
Gorovitz
Batia
B
Rawat
Mamta
M
Fahey
Robert C
RC
Av-Gay
Yossef
Y
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D003545:Cysteine; D004251:DNA Transposable Elements; D004187:Disaccharides / Q000096:biosynthesis*; D006020:Glycopeptides; D016695:Glycosyltransferases / Q000235:genetics* / Q000378:metabolism*; D007294:Inositol; D008969:Molecular Sequence Data; D009154:Mutation; D020102:Mycobacterium smegmatis / Q000201:enzymology* / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000201:enzymology* / Q000235:genetics; D011720:Pyrazoles; D016415:Sequence Alignment; D013438:Sulfhydryl Compounds
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004187:Disaccharides; D006020:Glycopeptides; D011720:Pyrazoles; D013438:Sulfhydryl Compounds; C089265:mycothiol; D007294:Inositol; D016695:Glycosyltransferases; D003545:Cysteine