title: Sensitive flow cytometric analysis reveals a novel type of parent-of-origin effect in the mouse genome.
abstract: The discovery of classic parental imprinting came, at least in part, from the analysis of transgene expression in mice. It was noticed that some transgenes were only expressed following paternal transmission and that others sometimes showed differential patterns of methylation depending on the parent of origin. Here, we present evidence of a novel and more subtle form of parental imprinting by taking advantage of the highly sensitive detection of murine transgene expression afforded by flow cytometry. We have produced nine lines of transgenic mice carrying a GFP reporter linked to the human alpha-globin promoter and enhancer elements, which direct expression to erythroid cells. A high proportion of transgenic lines, four of the nine, display significantly lower levels of expression following maternal transmission. Both the percentage of expressing cells and the mean fluorescence in expressing cells are between 10% and 30% lower following maternal transmission. These effects are reversible upon passage through the opposite germline. This finding raises the possibility that differences in the epigenetic state of the maternal and paternal chromosomes in adult somatic cells are more widespread than was previously thought.
authors.affiliation: School of Molecular and Microbial Biosciences, Building G08, The University of Sydney, Sydney, NSW 2006, Australia.
authors.forename: Jost I
authors.initials: JI
authors.lastname: Preis
authors.forename: Meredith
authors.initials: M
authors.lastname: Downes
authors.forename: Nathan A
authors.initials: NA
authors.lastname: Oates
authors.forename: John E J
authors.initials: JE
authors.lastname: Rasko
authors.forename: Emma
authors.initials: E
authors.lastname: Whitelaw
mesh_terms: D000818:Animals; D002469:Cell Separation; D005434:Flow Cytometry / Q000379:methods; D017930:Genes, Reporter; D016678:Genome; D018392:Genomic Imprinting; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D051379:Mice; D008822:Mice, Transgenic; D010375:Pedigree; D012680:Sensitivity and Specificity; D019076:Transgenes / Q000235:genetics*
chemical_list: D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins