Cytotoxicity of bisphenol A glycidyl methacrylate on cytochrome P450-producing cells.
Of the cytochrome P450 (CYP) family of carcinogen-activating enzymes, CYP3A is the major form found in human livers. The purpose of this study was to investigate the cytotoxic effects of dental resin monomers after being metabolized by CYP3A4 and CYP3A7, using a colony formation assay and a neutral red assay. Specimen wells were plated with transfected cells derived from the Chinese hamster lung at 100 cells well(-1). The experimental group consisted of CYP-producing 3A4-10 and 3A7-40 cells, while the control group consisted of non-CYP-producing CR-119 cells. Bisphenol A (BPA) and bisphenol A glycidyl methacrylate (Bis-GMA) and a positive control (Aflatoxine Bl) were added separately to each well and cultured for 7 days. After cultivation, the number of the colonies was counted and IC50 values were determined. The data were statistically analysed by a Student's t-test. The resultant of IC50 values indicated that the monomers were not metabolically activated by CYP3A4 or CYP3A7 as compared with the control (P < 0.05). We also confirmed that these monomers act neither as activators nor as inhibitors of CYP3A4 and CYP3A7.
Hikage
S
S
Department of Fixed Prosthodontics, School of Dentistry, Health Sciences University of Hokkaido, Tobetsu-Cho, Ishikari-Gun, Japan. hikagesa@hoku-iryo-u.ac.jp
Nakayama
K
K
Saito
T
T
Takahashi
Y
Y
Kamataki
T
T
Suzuki
S
S
Hongo
T
T
Sato
A
A
D000348:Aflatoxins / Q000032:analysis*; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism; D001559:Benzhydryl Compounds; D017438:Bisphenol A-Glycidyl Methacrylate / Q000378:metabolism / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D019388:Cytochrome P-450 CYP1A2 / Q000378:metabolism; D051544:Cytochrome P-450 CYP3A; D003764:Dental Materials / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004968:Estrogens, Non-Steroidal / Q000032:analysis*; D010636:Phenols / Q000032:analysis*; D000098826:Bisphenol A Compounds
D000348:Aflatoxins; D001189:Aryl Hydrocarbon Hydroxylases; D001559:Benzhydryl Compounds; D017438:Bisphenol A-Glycidyl Methacrylate; D019388:Cytochrome P-450 CYP1A2; D051544:Cytochrome P-450 CYP3A; D003764:Dental Materials; D004968:Estrogens, Non-Steroidal; D010636:Phenols; C103123:CYP3A7 protein, human; C006780:bisphenol A; D000098826:Bisphenol A Compounds