A novel colorimetric method to quantify tannase activity of viable bacteria.
A novel colorimetric method to quantify tannase activity of viable tannase-producing bacterial strains was developed through application of a visual reading method that was to detect the activity qualitatively. The novel method was sensitive enough to quantify the marginal tannase activity of strains that could not be otherwise measured by conventional spectrophotometric or colorimetric methods.
Nishitani
Yosuke
Y
Department of Bioscience, Graduate School of Science, Kobe University, Rokkodai 1-1, Nada-ku, 657-8501, Kobe City, Japan.
Osawa
Ro
R
D001419:Bacteria / Q000201:enzymology*; D002265:Carboxylic Ester Hydrolases / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism*; D003124:Colorimetry / Q000379:methods*; D003470:Culture Media; D005707:Gallic Acid / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000145:classification / Q000201:enzymology / Q000378:metabolism; D013053:Spectrophotometry / Q000379:methods; D013291:Streptococcus / Q000145:classification / Q000201:enzymology / Q000378:metabolism
D003470:Culture Media; D005707:Gallic Acid; D002265:Carboxylic Ester Hydrolases; C022711:tannase