[Primary study of Na+ channels in the epithelial cells of nasal polyps and of the effect of IL-1 beta on Na+ channels].
OBJECTIVE
To investigate the relationship between Na+ channels and nasal polyps and to study the effect of IL-1 beta on Na+ channels of epithelial cells of human normal nasal turbinate (NT) and nasal polyps (NP).


METHODS
Serum-free cultured epithelial cells of NT from obstructive sleep apnea syndrome patients and NP were studied on collagen gel-coated membranes at an air-liquid interface using the patch-clamp technique in the cell-attached configuration.


RESULTS
(1) The three Na+ absorption-related factors(the incidence of Na+ channels, the conductance of Na+ channels and the open probability of Na+ channels) were compared in NT cells and NP cells. There was no difference between NT and NP cells in the incidence(P > 0.05) and in the conductance(P > 0.05). However, the open probability was higher in NP cells compared to NT cells(P < 0.01). (2) Application of IL-1 beta increased significantly in the open probability of Na+ channels in NT and in NP cells (P < 0.01), the conductance of Na+ channels in NP cells was strongly increased by IL-1 beta(100 ng/ml)(P < 0.01).


CONCLUSIONS
(1) The formation of NP is significantly correlated with increased Na+ absorption, the prolonged open time of Na+ channels is one of the most important factors. (2) IL-1 beta may upregulate Na+ permeability by prolonged open time of Na+ channels and increased conductance of Na+ channels, increased absorption of Na+ may lead to the growth and enlargement of NP.
Zhang
X
X
Department of Otorhinolaryngology, Third Teaching Hospital, Bethune University of Medical Sciences, Changchun 130031, China. zhxinxin@163.net
Guo
Y
Y
Zhang
W
W
Dong
Z
Z
Yang
Z
Z
D000293:Adolescent; D000328:Adult; D004847:Epithelial Cells / Q000187:drug effects* / Q000502:physiology; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D015222:Sodium Channels / Q000187:drug effects* / Q000502:physiology; D014420:Turbinates / Q000187:drug effects / Q000502:physiology
D007375:Interleukin-1; D015222:Sodium Channels