[Local treatment with human laryngeal squamous carcinoma draining lymph node lymphocytes in nude mice bearing human laryngeal carcinoma xenografts].
OBJECTIVE
To increase the number of the tumor draining lymph nodes lymphocytes (TDLNLs) in tumor site and study its anti-tumor effect.


METHODS
Interleukin 2-activated TDLNLs were administrated by local injection in 10 nude mice bearing human laryngeal cancer at 10(7) cells/mouse, once a week for two weeks. As a control, 10 nude mice bearing human laryngeal cancer were injected with D-Hanks buffer solution.


RESULTS
The volume and weight of TDLNLs treated tumors were significantly reduced in comparison with those of the control (P < 0.05). The observed histopathologic characteristics of treated tumors were tumor necrotic foci and increased infiltration of lymphocytes in tumor and around tumor vessels. Proliferation of spleen nodules was also observed in the treatment group. Remote metastasis was not found in this group. In contrast with the control group, tumor necrosis and proliferation of spleen nodules were not observed and infiltration of lymphocytes in the tumor was not significant. There was a lung metastasis in the control group.


CONCLUSIONS
Local injection of TDLNLs is effective against human laryngeal carcinoma xenografts in nude mice. It also could stimulate the immunoresponse of hosts and probably decrease the chance of tumor metastasis.
Yan
Y
Y
Department of Otorhinolaryngology, First Affiliated Hospital, Beijing Medical University, Beijing 100034.
Han
D
D
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000628:therapy*; D006801:Humans; D016219:Immunotherapy, Adoptive / Q000379:methods*; D007376:Interleukin-2 / Q000494:pharmacology; D007822:Laryngeal Neoplasms / Q000473:pathology / Q000628:therapy*; D008198:Lymph Nodes / Q000473:pathology; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation
D007376:Interleukin-2