The gene encoding glutathione-dependent formaldehyde dehydrogenase/GSNO reductase is responsive to wounding, jasmonic acid and salicylic acid.
It has recently been discovered that glutathione-dependent formaldehyde dehydrogenase (FALDH) exhibits a strong S-nitrosoglutathione reductase activity. Plants use NO and S-nitrosothiols as signaling molecules to activate defense mechanisms. Therefore, it is interesting to investigate the regulation of FALDH by mechanical wounding and plant hormones involved in signal transduction. Our results show that the gene encoding FALDH in Arabidopsis (ADH2) is down-regulated by wounding and activated by salicylic acid (SA). In tobacco, FALDH levels and enzymatic activity decreased after jasmonate treatment, and increased in response to SA. This is the first time that regulation of FALDH in response to signals associated with plant defense has been demonstrated.
Díaz
Maykelis
M
Departamento de Bioqui;mica y Biología Molecular, Facultad de Ciencias, Universidad Autónoma de Barcelona, 08193, Barcelona, Bellaterra, Spain.
Achkor
Hakima
H
Titarenko
Elena
E
Martínez
M Carmen
MC
D000445:Aldehyde Oxidoreductases / Q000096:biosynthesis / Q000235:genetics*; D017360:Arabidopsis / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D002460:Cell Line; D003517:Cyclopentanes / Q000494:pharmacology*; D004790:Enzyme Induction; D004794:Enzyme Repression; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005980:Glutathione Reductase / Q000096:biosynthesis / Q000235:genetics*; D054883:Oxylipins; D026422:S-Nitrosoglutathione / Q000378:metabolism; D020156:Salicylic Acid / Q000494:pharmacology*; D013314:Stress, Mechanical; D014026:Nicotiana / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D014158:Transcription, Genetic
D003517:Cyclopentanes; D054883:Oxylipins; D026422:S-Nitrosoglutathione; C011006:jasmonic acid; C020145:formaldehyde dehydrogenase (glutathione); D000445:Aldehyde Oxidoreductases; D005980:Glutathione Reductase; D020156:Salicylic Acid