title: Identification of differentially expressed mRNAs of the autologous vein graft after an arterial bypass in mongrel dogs.
abstract: OBJECTIVE
  Intimal hyperplasia (IH) is characterized by vascular smooth muscle cell (VSMC) proliferation in the intima and subsequent accumulation of extracellular matrix. A variety of factors that might be considered as possible VSMC mitogens induce the specific gene expression of VSMC. This study was designed to identify differentially expressed mRNA by method using an mRNA differential display.
  
  
  METHODS
  A bilateral femoropopliteal reverse saphenous vein bypass was performed on both hind limbs of mongrel dogs. At 4 and 8 weeks after the implantation, the vein bypass grafts were harvested. The total RNAs were extracted from each specimen and transcribed into the cDNAs. Amplified cDNAs using 16 sets of primers were separated on DNA sequencing gel. Differentially displayed bands were excised from the gel, cloned, and then sequenced. The sequences were compared with the National Center of Biotechnology Information nonredundant sequence database using the Basic Local Alignment Search Tool (BLAST) program.
  
  
  RESULTS
  Forty-three bands were cloned and sequenced. A computer search revealed that 11 of them had similarities to known genes while 32 cDNAs had no similarities to any registered DNA sequence.
  
  
  CONCLUSIONS
  We found several genes to be related to IH. Some of them had already had their function and sequence identified whereas some of them have yet to be identified. Further studies are necessary to determine the precise relationship between these genes and IH.
authors.affiliation: Division of Vascular Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50 Ilwon-Dong, Kangnam-Ku, Seoul 135-710, South Korea.
authors.forename: Dong-Ik
authors.initials: DI
authors.lastname: Kim
authors.forename: Ji-Eun
authors.initials: JE
authors.lastname: Kim
authors.forename: Se-Ho
authors.initials: SH
authors.lastname: Huh
authors.forename: Jung-Hwan
authors.initials: JH
authors.lastname: Park
authors.forename: Byung-Boong
authors.initials: BB
authors.lastname: Lee
authors.forename: Jin-Soo
authors.initials: JS
authors.lastname: Han
authors.forename: Jong-Sung
authors.initials: JS
authors.lastname: Kim
authors.forename: Min-Jae
authors.initials: MJ
authors.lastname: Lee
authors.forename: In-Sung
authors.initials: IS
authors.lastname: Jang
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D001158:Arteries / Q000601:surgery; D004285:Dogs; D015870:Gene Expression; D020869:Gene Expression Profiling; D006965:Hyperplasia; D008297:Male; D008934:Mitogens / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D012501:Saphenous Vein / Q000637:transplantation; D017539:Tunica Intima / Q000473:pathology*; D014680:Veins / Q000473:pathology / Q000637:transplantation*
chemical_list: D008934:Mitogens; D012333:RNA, Messenger