Identification of novel polymorphisms in the Adam33 gene.
Adam33 is a member of a family of genes that encode membrane-anchored proteins with a disintegrin and a metalloprotease domain and that are primarily expressed in lung fibroblasts and bronchial smooth muscle. The human Adam33 gene is located on chromosome 20p13, a region that has been linked to asthma and bronchial hyperresponsiveness. Recently, the polymorphisms in Adam33 have been found to be associated with asthma. In this study, we performed polymorphism scanning of the entire genomic region, including the promoter region of Adam33, by direct sequencing. We identified 16 novel polymorphisms in the Adam33 gene. Among these novel polymorphisms, three polymorphisms (-2154G-->A, -753T-->A, and -330C-->T) were found to be in the promoter region and one polymorphism (13491 G-->A) was located in 3' untranslated region of the Adam33 gene.
Chae
Soo-Cheon
SC
Genomic Research Center for Immune Disorders, Wonkwang University School of Medicine, Iksan, 570-749, Chonbuk, Korea.
Yoon
Kwon-Ha
KH
Department of Radiology, Wonkwang University School of Medicine, Iksan, 570-749, Chonbuk, Korea.
Chung
Hun-Taeg
HT
Genomic Research Center for Immune Disorders, Wonkwang University School of Medicine, Iksan, 570-749, Chonbuk, Korea. htchung@wonnms.wonkwang.ac.kr.|Department of Microbiology and Immunology, Wonkwang University School of Medicine, Iksan, 570-749, Chonbuk, Korea. htchung@wonnms.wonkwang.ac.kr.
D020413:3' Untranslated Regions; D051722:ADAM Proteins; D044466:Asian People / Q000235:genetics; D001249:Asthma / Q000235:genetics; D016535:Bronchial Hyperreactivity / Q000235:genetics; D002890:Chromosomes, Human, Pair 20; D004247:DNA / Q000235:genetics; D005787:Gene Frequency; D006801:Humans; D008666:Metalloendopeptidases / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA
D020413:3' Untranslated Regions; D004247:DNA; D051722:ADAM Proteins; C450441:ADAM33 protein, human; D008666:Metalloendopeptidases