A single mutation of restriction endonuclease EcoRII led to a new crystal form that diffracts to 2.1 A resolution.
R88A, a mutant of the type IIE restriction endonuclease EcoRII, has been crystallized in space group P2(1), with unit-cell parameters a = 58.7, b = 92.4, c = 88.3 A, beta = 108.1 degrees. There are two monomers in the asymmetric unit and the solvent content is estimated to be 50% by volume. The crystals diffract to 2.1 A resolution, which is much higher than that of the wild type, which diffracted to 2.8 A resolution. The mutant crystals have been used in the identification of an excellent heavy-atom derivative.
Zhou
Xiaoyin E
XE
Laboratory for Structural Biology, Department of Chemistry, University of Alabama in Huntsville, Huntsville, AL 35899, USA.
Wang
Yujun
Y
Reuter
Monika
M
Mackeldanz
Petra
P
Krüger
Detlev H
DH
Meehan
Edward J
EJ
Chen
Liqing
L
D019943:Amino Acid Substitution; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D015252:Deoxyribonucleases, Type II Site-Specific / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006046:Gold / Q000737:chemistry; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics
D011994:Recombinant Proteins; D006046:Gold; C046545:CCWGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific