[Influence of super high molecular weight poly D,L-lactic acid on viability and new bone formation of osteoblasts].
OBJECTIVE
To investigate the influence of the viability and new bone formation of osteoblasts by the super high molecular weight poly D,L-lactic acid (SHMW-PDLLA).


METHODS
1. The osteoblasts derived from neonatal rat were grown and maintained at steep of SHMW-PDLLA and normal culture medium. The viability and function of the osteoblasts were measured with MTT array. 2. The plate and screws made of SHMW-PDLLA were implanted and fixed at the artificial fractured mandible of dogs. Specimens were gained at 3 and 6 months and examined with macroscopy and SEM.


RESULTS
1. There is no significant difference of OD values between the experimental group and the control group (P > 0.05). The SHMW-PDLLA isn't toxic to osteoblast at 1 week and 2 weeks, and the toxicity is 3% at 3 days. 2. There were a lot of new bone formed between the implanted SHMW-PDLLA plate and bone tissues under SEM.


CONCLUSIONS
SHMW-PDLLA hasn't pathological influence on the viability and new bone formation of osteoblasts and it is feasible in tissue engineering of bone.
Wei
Shi-cheng
SC
Department of Oral and Maxillofacial Surgery, West China College of Stomatology, Sichuan University, Chengdu 610041, China.
Zheng
Qian
Q
Liu
Lei
L
Li
Sheng-wei
SW
Wang
Han-zhang
HZ
Xiong
Cheng-dong
CD
D000818:Animals; D000831:Animals, Newborn; D001860:Bone Plates; D001863:Bone Screws; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D004285:Dogs; D019344:Lactic Acid / Q000737:chemistry / Q000494:pharmacology*; D008337:Mandibular Fractures / Q000601:surgery; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D019647:Oral Surgical Procedures / Q000295:instrumentation / Q000379:methods; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D010012:Osteogenesis / Q000187:drug effects*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats
D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)