title: Kinetics and postmucosal effects on urinary recovery of 5 intravenously administered sugars in healthy cats.
abstract: The objective of this study was to describe the kinetics of urinary recovery and to evaluate the effects of postmucosal factors on urinary recovery of 5 intravenously administered saccharides. Ten cats received an isotonic sugar solution containing lactulose, rhamnose, xylose, methylglucose, and sucrose intravenously. These sugars were selected because of their prior use for intestinal permeability and mucosal function testing in humans and dogs. Urethral catheterization with a closed collection system was used for collection of cumulative urine samples prior to and 2, 4, 6, 8, 10, 12, and 24 h after administration of the sugar solution. High-pressure anion exchange liquid chromatography with pulsed amperometric detection was used to measure the concentrations of each sugar in the urine and calculate urinary recovery. Twenty-four hour cumulative urinary recovery for each sugar from the cats, was lower than expected compared to dogs and humans. All 5 sugars had the highest percentage of urinary recovery during the first 2 h after administration. Mean sugar elimination rate constants and half-lives ranged from 0.268/h for methylglucose to 0.415/h for lactulose and 1.67 h for lactulose to 2.59 h for methylglucose, respectively. Metabolism and incomplete urine collection are possible reasons for lower cumulative urinary recoveries of these 5 sugars in cats compared with dogs. Although these 5 sugars are not ideal marker molecules, they may still be useful for intestinal permeability and mucosal function testing in cats.
authors.affiliation: Animal Health Center, College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi 39762-9825, USA. mattdvm97@yahoo.com
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Krecic
authors.forename: Jörg M
authors.initials: JM
authors.lastname: Steiner
authors.forename: Margaret R
authors.initials: MR
authors.lastname: Kern
authors.forename: David A
authors.initials: DA
authors.lastname: Williams
mesh_terms: D019325:3-O-Methylglucose / Q000493:pharmacokinetics / Q000652:urine; D000818:Animals; D015415:Biomarkers / Q000652:urine; D002241:Carbohydrates / Q000493:pharmacokinetics* / Q000652:urine*; D002415:Cats / Q000378:metabolism* / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004068:Digestive System Physiological Phenomena; D004285:Dogs; D005765:Gastrointestinal Agents / Q000493:pharmacokinetics* / Q000652:urine*; D005947:Glucose / Q000493:pharmacokinetics; D006801:Humans; D007275:Injections, Intravenous / Q000662:veterinary; D007792:Lactulose / Q000493:pharmacokinetics / Q000652:urine; D008297:Male; D051381:Rats; D012210:Rhamnose / Q000493:pharmacokinetics / Q000652:urine; D013395:Sucrose / Q000493:pharmacokinetics / Q000652:urine; D016482:Urinalysis / Q000662:veterinary; D014546:Urinary Catheterization / Q000662:veterinary; D014994:Xylose / Q000493:pharmacokinetics / Q000652:urine
chemical_list: D015415:Biomarkers; D002241:Carbohydrates; D005765:Gastrointestinal Agents; D019325:3-O-Methylglucose; D007792:Lactulose; D013395:Sucrose; D014994:Xylose; D005947:Glucose; D012210:Rhamnose