[Genetic polymorphism of six short tandem repeat loci in the Han population in Hebei province of China].
OBJECTIVE
This article reports a population genetic study on six short tandem repeat(STR) loci, D7S820, D19S253, D12S391, D5S818, D16S539 and D8S1179, in a sample of unrelated Chinese Han individuals(n=122-173) living in Hebei province.


METHODS
DNA extraction from blood samples (200 in number) and multiplex amplification of the above six loci were carried out. Using denaturing polyacrylamide gel electrophoresis and silver stain, the authors investigated the distribution of allele frequencies of the six loci in Han population in Hebei province.


RESULTS
The STR polymorphisms at all of the six loci were observed in Chinese Han population in Hebei province. The observed heterozygosities of D7S820, D19S253, D12S391, D5S818, D16S539 and D8S1179 were 0.828, 0.757, 0.769, 0.837, 0.785 and 0.852, respectively. The measured values of the power of discrimination (PD) were 0.914, 0.919, 0.940, 0.909, 0.917, 0.944; of the mean exclusion chance(MEC) 0.618, 0.740, 0.801, 0.557, 0.655, 0.696 and of the polymorphism information content (PIC) in Chinese 0.771, 0.760, 0.762, 0.708, 0.776 and 0.794, respectively.


CONCLUSIONS
The genotype distributions of the six STR were in accordance with Hardy-Weinberg equilibrium. The numerical values of the PD and MEC are relatively high in Hebei province, and thus can be of significant application in population genetics and forensic medicine.
Lu
Ping
P
Maternal and Child Health Hospital of Shijiazhuang, Shijiazhuang, Hebei, 050051 PR China.
Zhao
Qing-guo
QG
Liu
Yu-lai
YL
Yu
Yu-lu
YL
Zhu
Ai-ping
AP
Li
Qing
Q
Di
Shu-lan
SL
Feng
Ji-zhen
JZ
Zhang
Jun-ge
JG
Li
Chong-jie
CJ
D044466:Asian People / Q000235:genetics; D002681:China / Q000208:ethnology; D004247:DNA / Q000032:analysis; D005260:Female; D005787:Gene Frequency; D005828:Genetics, Population; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic; D044382:Population Groups; D020080:Tandem Repeat Sequences / Q000235:genetics / Q000502:physiology
D004247:DNA