title: Helicobacter pylori supernatants cause epithelial cytoskeletal disruption that is bacterial strain and epithelial cell line dependent but not toxin VacA dependent.
abstract: We show here that Helicobacter pylori broth culture supernatants disrupt the actin cytoskeleton of epithelial cell lines, leading to cell rounding and apoptosis through anoikis. We demonstrate that there are marked quantitative differences between strains and that there are different cell line sensitivities. By constructing VacA null isogenic mutants, we show that the effect is not due to the vacuolating cytotoxin.
authors.affiliation: Division of Gastroenterology and Institution of Infections, Immunity and Inflammation, University of Nottingham, University Hospital, United Kingdom.
authors.forename: James R
authors.initials: JR
authors.lastname: Bebb
authors.forename: Darren P
authors.initials: DP
authors.lastname: Letley
authors.forename: Joanne L
authors.initials: JL
authors.lastname: Rhead
authors.forename: John C
authors.initials: JC
authors.lastname: Atherton
mesh_terms: D000818:Animals; D017209:Apoptosis; D001426:Bacterial Proteins / Q000502:physiology*; D002460:Cell Line; D003599:Cytoskeleton / Q000473:pathology*; D004847:Epithelial Cells / Q000473:pathology; D016480:Helicobacter pylori / Q000472:pathogenicity*; D006801:Humans; D010641:Phenotype
chemical_list: D001426:Bacterial Proteins; C074689:VacA protein, Helicobacter pylori