[Determination of piceid in commercial Rhizoma Polygoni Cuspidati by HPLC].
OBJECTIVE
To improve a RP-HPLC method of determining piceid in commercial Rhizoma Polygoni Cuspidati.


METHODS
HPLC method was used, with C18 column (250 mm x 4.6 mm), a mixture of CH3CN-H2O (20:80) as the mobile phase, and detecting wavelength 303 nm.


RESULTS
The linear range was 0.0066-0.0792 microgram.microL-1, r = 0.9999 (n = 5). The average recovery rate was 98.5% and RSD was 2.5% (n = 9).


CONCLUSIONS
The method is simple, accurate and reproducible. The contents of piceid in commercial Rhizoma Polygoni Cuspidati from different areas are variable and the average content is 2.23%.
Wang
Lei
L
Department of Pharmacognosy, China Pharmaceutical University, Nanjing, 210038, Jiangsu, China.
Huang
Lan
L
Zhang
Mian
M
Wang
Zheng-tao
ZT
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D005960:Glucosides / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D028502:Polygonum / Q000737:chemistry*; D011786:Quality Control; D027343:Rhizome / Q000737:chemistry; D013045:Species Specificity; D013267:Stilbenes / Q000032:analysis*
D004365:Drugs, Chinese Herbal; D005960:Glucosides; D013267:Stilbenes; C058229:polydatin