title: Endoventricular porcine autologous myoblast transplantation can be successfully achieved with minor mechanical cell damage.
abstract: OBJECTIVE
  Transplantation of skeletal myogenic precursor cells (mpc) into the myocardium using a non-surgical procedure.
  
  
  METHODS
  Closed-chest mpc transplantation was assessed in pigs using the NOGA-Biosense device allowing both electromechanical mapping of the left ventricle (LV), and guided mpc injections through endocardium.
  
  
  RESULTS
  We successively established that: (1) adequate preimplantation handling of mpc can be achieved when mpc are kept in 0.1% serum albumin-containing medium until implantation; (2) mpc are neither retained nor destroyed in the catheter or the needle and their passage does not affect their survival, growth and differentiation; (3) large numbers of autologous mpc can be actually transplanted in the LV myocardium by transendocardial route, as assessed by post-mortem examination of pigs injected with iron-loaded mpc; (4) cell injection into the myocardium does not induce conspicuous cell mortality since more than 80% of mpc recovered from LV tissue are alive 15 min after injection; (5) mpc injections can be guided into circumscribed LV targets such as infarcted areas, as assessed by comparison of map injection sites with location of iron-loaded mpc at post-mortem examination of LV myocardium.
  
  
  CONCLUSIONS
  This new approach may pave the way for a large spectrum of cell therapies targeting myocardial diseases.
authors.affiliation: INSERM E0011, Faculté de Médecine, Créteil, France.
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Chazaud
authors.forename: Luc
authors.initials: L
authors.lastname: Hittinger
authors.forename: Corinne
authors.initials: C
authors.lastname: Sonnet
authors.forename: Stéphane
authors.initials: S
authors.lastname: Champagne
authors.forename: Philippe
authors.initials: P
authors.lastname: Le Corvoisier
authors.forename: Nicole
authors.initials: N
authors.lastname: Benhaiem-Sigaux
authors.forename: Thierry
authors.initials: T
authors.lastname: Unterseeh
authors.forename: Jinbo
authors.initials: J
authors.lastname: Su
authors.forename: Pascal
authors.initials: P
authors.lastname: Merlet
authors.forename: Alain
authors.initials: A
authors.lastname: Rahmouni
authors.forename: Jérôme
authors.initials: J
authors.lastname: Garot
authors.forename: Romain
authors.initials: R
authors.lastname: Gherardi
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Teiger
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002470:Cell Survival; D004594:Electrophysiology; D006333:Heart Failure / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D006352:Heart Ventricles; D007267:Injections; D023421:Models, Animal; D032448:Myoblasts, Skeletal / Q000473:pathology / Q000637:transplantation*; D009203:Myocardial Infarction / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D013552:Swine; D014182:Transplantation, Autologous