title: Whey proteins as a model system for chromatographic separation of proteins.
abstract: Although chromatographic separation of whey proteins has been considered too expensive, whey may serve as an excellent model mixture to investigate and validate the use of simulation tools in the development and optimization of chromatographic separations and the outcome could easily be utilized since the model system has an intrinsic value. Besides, milk from transgenic animals could be an attractive source of pharmaceuticals which must be separated from the other proteins in the milk. Several whey proteins are of interest especially, alpha-lactalbumin, beta-lactoglobulins, immunoglobulins, lactoperoxidase, and lactoferrin. The scope of the project is to develop a consistent set of chromatographic data for whey proteins including isotherms, transport properties and scale-up studies and to develop the appropriate models for the anion exchangers Q-Sepharose XL, Source 30Q, Ceramic Q-HyperD F, and Merck Fractogel EMD TMAE 650 (S). In this work we have determined and correlated gradient and isocratic retention volumes in the linear range of the isotherm for alpha-lactalbumin, beta-lactoglobulin A and B, and bovine serum albumin at a pH from 6 to 9 at various NaCl concentrations.
authors.affiliation: Engineering Research Centre IVC-SEP, Department of Chemical Engineering, Technical University of Denmark, Building 229, DK-2800, Lyngby, Denmark.
authors.forename: Linda
authors.initials: L
authors.lastname: Pedersen
authors.forename: Jørgen
authors.initials: J
authors.lastname: Mollerup
authors.forename: Ernst
authors.initials: E
authors.lastname: Hansen
authors.forename: Alois
authors.initials: A
authors.lastname: Jungbauer
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D008894:Milk Proteins / Q000302:isolation & purification*; D008956:Models, Chemical; D000067816:Whey Proteins
chemical_list: D008894:Milk Proteins; D000067816:Whey Proteins