Overexpression of beta-1,4-galactosyltransferase I in rat Schwann cells promotes the growth of co-cultured dorsal root ganglia.
The cell surface beta-1,4-galactosyltransferase I (beta-1,4-GalT-I) functions as one of the receptors of laminin during the neurite outgrowth on basal lamina by binding to N-linked oligosaccharides in the laminin E8 domain. In this study, we demonstrated that the purified rat Schwann cells transfected with the expression plasmid of beta-1,4-GalT-I cDNA transiently promoted outgrowth and elongation of the neurites from co-cultured rat dorsal root ganglia, while those transfected with the antisense expression plasmid of beta-1,4-GalT-I had the opposite effects. These results suggested that the expression of beta-1,4-GalT-I in Schwann cells of peripheral nerve might promote both growth of developmental neuron and regeneration of injured nerve.
Shen
Aiguo
A
Box 103, Gene Research Center, Shanghai Medical College of Fudan University (Former Shanghai Medical University), 200032, Shanghai, People's Republic of China.
Yan
Jun
J
Ding
Fei
F
Gu
Xiaosong
X
Zhu
Dan
D
Gu
Jianxin
J
D000818:Animals; D000831:Animals, Newborn; D015152:Blotting, Northern / Q000379:methods; D046508:Culture Techniques / Q000379:methods*; D016373:DNA, Antisense / Q000378:metabolism; D005333:Fetus; D005727:Ganglia, Spinal / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods; D009237:N-Acetyllactosamine Synthase / Q000378:metabolism / Q000502:physiology*; D016501:Neurites / Q000502:physiology*; D010949:Plasma; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000201:enzymology* / Q000378:metabolism; D012584:Sciatic Nerve; D013997:Time Factors; D014162:Transfection / Q000379:methods
D016373:DNA, Antisense; D009237:N-Acetyllactosamine Synthase