Cloning and characterization of a glucose 6-phosphate/phosphate translocator from Oryza sativa.
Plastids of nongreen tissues import carbon as a source of biosynthetic pathways and energy, and glucose 6-phosphate is the preferred hexose phosphate taken up by nongreen plastids. A cDNA clone encoding glucose 6-phosphate/phosphate translocator (GPT) was isolated from a cDNA library of immature seeds of rice and named as OsGPT. The cDNA has one uninterrupted open reading frame encoding a 42 kDa polypeptide possessing transit peptide consisting of 70 amino acid residues. The OsGPT gene maps on chromosome 8 of rice and is linked to the quantitative trait locus for 1000-grain weight. The expression of OsCPT is mainly restricted to heterotrophic tissues. These results suggest that glucose 6-phosphate imported via GPT can be used for starch biosynthesis in rice nongreen plastids.
Jiang
Hua-Wu
HW
The State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310029, China.
Dian
Wei-Min
WM
Liu
Fei-Yan
FY
Wu
Ping
P
D000595:Amino Acid Sequence; D017920:Antiporters / Q000096:biosynthesis* / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009004:Monosaccharide Transport Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D012275:Oryza / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D012639:Seeds / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017385:Sequence Homology; D013045:Species Specificity; D013213:Starch / Q000096:biosynthesis*
D017920:Antiporters; D009004:Monosaccharide Transport Proteins; D011994:Recombinant Proteins; C111261:glucose 6-phosphate(transporter); D013213:Starch