Preparation and crystallization of human flap endonuclease FEN-1 in complex with proliferating-cell nuclear antigen, PCNA.
Flap endonuclease-1 (FEN-1) is a structure-specific nuclease that removes 5'-overhanging flaps in DNA repair and replication. FEN-1 binds proliferating-cell nuclear antigen (PCNA), a DNA-clamp protein, when processing Okazaki fragments during lagging-strand DNA synthesis. Here, the crystallization of the complex between human FEN-1 and PCNA is reported. The crystals were found to belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 82.2, b = 143.4, c = 246.7 A, and contained one complex in the crystallographic asymmetric unit. A diffraction data set was collected to a resolution of 3.0 A.
Sakurai
Shigeru
S
Structural Biology Laboratory, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.
Kitano
Ken
K
Okada
Kengo
K
Hamada
Keisuke
K
Morioka
Hiroshi
H
Hakoshima
Toshio
T
D003460:Crystallization; D004591:Electrophoresis, Polyacrylamide Gel; D045585:Flap Endonucleases / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D018809:Proliferating Cell Nuclear Antigen / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D014961:X-Ray Diffraction
D018809:Proliferating Cell Nuclear Antigen; D011994:Recombinant Proteins; D045585:Flap Endonucleases