title: Retinal expression of clusterin in the streptozotocin-induced diabetic rat.
abstract: To assess the possible relevance of clusterin in the pathophysiology of retinopathy associated with diabetes mellitus, streptozotocin-induced diabetic rats were studied. Clusterin expression was measured in both normal and streptozotocin-induced diabetic rat retinas using Northern blotting, reverse transcription polymerase chain reaction, immunohistochemistry and Western blot analysis. The results show increased clusterin protein level and its mRNA expression 6 weeks after induction of diabetes. Clusterin was localized to the inner nuclear and ganglion cell layers of both normal and diabetic rat retinas. These data show that diabetes affects the expression of clusterin in the retina and may reflect a diabetes-induced damage and/or alterations of neural structures resulting in diabetic retinopathy.
authors.affiliation: Department of Anatomy and Neurobiology, Institute of Health Science, College of Medicine, Gyeongsang National University, Chilam-dong 92, Jinju, Kyungnam 660-751, South Korea.
authors.forename: Yoon Sook
authors.initials: YS
authors.lastname: Kim
authors.forename: Young Hee
authors.initials: YH
authors.lastname: Kim
authors.forename: Eun Woo
authors.initials: EW
authors.lastname: Cheon
authors.forename: Jong Moon
authors.initials: JM
authors.lastname: Park
authors.forename: Ji Myong
authors.initials: JM
authors.lastname: Yoo
authors.forename: Sang Soo
authors.initials: SS
authors.lastname: Kang
authors.forename: Gyeong Jae
authors.initials: GJ
authors.lastname: Cho
authors.forename: Wan Sung
authors.initials: WS
authors.lastname: Choi
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D015152:Blotting, Northern; D015153:Blotting, Western; D001835:Body Weight; D051152:Clusterin; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D003930:Diabetic Retinopathy / Q000378:metabolism* / Q000473:pathology; D006023:Glycoproteins / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D018832:Molecular Chaperones / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000378:metabolism* / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D001786:Blood Glucose; D051152:Clusterin; D006023:Glycoproteins; D018832:Molecular Chaperones