title: Cytogenetic evolution of human ovarian cell lines associated with chemoresistance and loss of tumorigenicity.
abstract: In order to identify genomic changes associated with a resistant phenotype acquisition, we used comparative genomic hybridization (CGH) to compare a human ovarian cell line, Igrov1, and four derived subcell lines, resistant to vincristine and presenting a reversion of malignant properties. Multicolor FISH (Multiplex-FISH and Spectral Karyotype) and conventional FISH are also used to elucidate the karyotype of parental cell line. The drug-resistant subcell lines displayed many chromosomal abnormalities suggesting the implication of different pathways leading to a multidrug resistance phenotype. However, these cell lines shared two common rearrangements: an unbalanced translocation der(8)t(8;13)(p22;q?) and a deletion of the 11p. These chromosomal imbalances could reflected the acquisition of the chemoresistance (der(8)) or the loss of tumorigenicity properties (del(11p)).
authors.affiliation: Laboratory of Hematology, Robert Debré Hospital and Medical Faculty (UPRES EA 20-70-IFR 53 Biomolecules), 51100 Reims, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Struski
authors.forename: Martine
authors.initials: M
authors.lastname: Doco-Fenzy
authors.forename: Michael
authors.initials: M
authors.lastname: Koehler
authors.forename: Ilse
authors.initials: I
authors.lastname: Chudoba
authors.forename: Francis
authors.initials: F
authors.lastname: Levy
authors.forename: Linda
authors.initials: L
authors.lastname: Masson
authors.forename: Nicole
authors.initials: N
authors.lastname: Michel
authors.forename: Evelyne
authors.initials: E
authors.lastname: Ulrich
authors.forename: Nadine
authors.initials: N
authors.lastname: Gruson
authors.forename: Jean
authors.initials: J
authors.lastname: Bénard
authors.forename: Gérard
authors.initials: G
authors.lastname: Potron
authors.forename: Pascale
authors.initials: P
authors.lastname: Cornillet-Lefebvre
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy* / Q000235:genetics* / Q000473:pathology; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D045744:Cell Line, Tumor; D002869:Chromosome Aberrations; D003582:Cytogenetics; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D010051:Ovarian Neoplasms / Q000188:drug therapy* / Q000235:genetics* / Q000473:pathology; D010641:Phenotype; D011123:Polyploidy; D014750:Vincristine / Q000494:pharmacology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D014750:Vincristine