Secondary losses via gamma-lactam formation in hot electron capture dissociation: a missing link to complete de novo sequencing of proteins?
Differentiating between the isomeric isoleucine and leucine amino acid residues can be accomplished by a gas-phase fragmentation reaction in which an N-terminal radical site of a C-terminal fragment initiates formation of a five-membered ring with the beta-carbon in the adjacent residue.
Kjeldsen
Frank
F
University of Southern Denmark, Department of Chemistry, Campusvej 55, DK-5230 Odense M, Denmark.
Zubarev
Roman
R
D000595:Amino Acid Sequence; D000818:Animals; D002364:Caseins / Q000737:chemistry*; D002417:Cattle; D005583:Fourier Analysis; D006023:Glycoproteins / Q000737:chemistry*; D007769:Lactams / Q000737:chemistry*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry*; D020539:Sequence Analysis, Protein / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D002364:Caseins; D006023:Glycoproteins; D007769:Lactams; C085522:PP3 protein, Bos taurus; D010446:Peptide Fragments