Early transcriptional effects of aldosterone in a mouse inner medullary collecting duct cell line.
The mineralocorticoid aldosterone is a major regulator of Na+ and acid-base balance and control of blood pressure. Although the long-term effects of aldosterone have been extensively studied, the early aldosterone-responsive genes remain largely unknown. Using DNA array technology, we have characterized changes in gene expression after 1 h of exposure to aldosterone in a mouse inner medullary collecting duct cell line, mIMCD-3. Results from three independent microarray experiments revealed that the expression of many transcripts was affected by aldosterone treatment. Northern blot analysis confirmed the upregulation of four distinct transcripts identified by the microarray analysis, namely, the serum and glucose-regulated kinase sgk, connective tissue growth factor, period homolog, and preproendothelin. Immunoblot analysis for preproendothelin demonstrated increased protein expression. Following the levels of the four transcripts over time showed that each had a unique pattern of expression, suggesting that the cellular response to aldosterone is complex. The results presented here represent a novel list of early aldosterone-responsive transcripts and provide new avenues for elucidating the mechanism of acute aldosterone action in the kidney.
Gumz
Michelle L
ML
Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL 32610-0245, USA.
Popp
Michael P
MP
Wingo
Charles S
CS
Cain
Brian D
BD
D000450:Aldosterone / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D055513:Connective Tissue Growth Factor; D019332:Endothelin-1; D016232:Endothelins / Q000235:genetics / Q000378:metabolism; D017874:Immediate-Early Proteins / Q000235:genetics; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics; D007679:Kidney Medulla; D007685:Kidney Tubules, Collecting / Q000502:physiology*; D051379:Mice; D009687:Nuclear Proteins; D020411:Oligonucleotide Array Sequence Analysis; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects*; D015854:Up-Regulation; D000099184:Serum-Glucocorticoid Regulated Kinases
D000450:Aldosterone; D055513:Connective Tissue Growth Factor; D019332:Endothelin-1; D016232:Endothelins; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D009687:Nuclear Proteins; D011498:Protein Precursors; D017346:Protein Serine-Threonine Kinases; D012333:RNA, Messenger; D000099184:Serum-Glucocorticoid Regulated Kinases; C526318:CCN2 protein, mouse