A simple PCR/RFLP analysis can differentiate between Candida albicans, Aspergillus niger, and Aspergillus fumigatus.
The clinical management of immunocompromised patients depends on the rapid identification of infectious agents such as fungal pathogens. The procedure described here for accomplishing this uses a sensitive polymerase chain reaction method, previously reported, combined with restriction-enzyme digestion to distinguish between Candida and Aspergillus species and to classify Aspergillus strains.
Isik
Nilgun
N
Department of Haematology, University of Wales College of Medicine, Cardiff CF14 4XN, Wales, UK.
White
Lewis
L
Barnes
Rosemary
R
Poynton
Christopher J
CJ
Mills
Ken I
KI
D001232:Aspergillus fumigatus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001234:Aspergillus niger / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001483:Base Sequence; D002176:Candida albicans / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D012680:Sensitivity and Specificity
D017931:DNA Primers