Infection competition against grouper nervous necrosis virus by virus-like particles produced in Escherichia coli.
Dragon grouper (Epinephelus lanceolatus) nervous necrosis virus (DGNNV) comprises 180 copies of capsid protein that encapsulate a bipartite genome of single-stranded (+)-RNAs. This study reports that virus-like particles (VLPs) are formed in Escherichia coli expressing the full-length ORF encoding the DGNNV capsid protein. Two sizes of VLPs are observed. The heavier particles resemble the native piscine nodavirus in size and stain permeability, while the lighter ones are approximately two-thirds of the full size. The recombinant VLPs block attachment of native virus to the surface of cultured fish nerve cells, blocking infection by the native virus.
Lu
Ming-Wei
MW
Institute of Marine Resources 804, National Sun Yat-Sen University, Kaohsiung, Taiwan.
Liu
Wangta
W
Institute of Marine Resources 804, National Sun Yat-Sen University, Kaohsiung, Taiwan.
Lin
Chan-Shing
CS
Institute of Marine Resources 804, National Sun Yat-Sen University, Kaohsiung, Taiwan.
D000818:Animals; D001483:Base Sequence; D001667:Binding, Competitive; D036022:Capsid Proteins / Q000235:genetics / Q000648:ultrastructure; D004279:DNA, Viral / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000821:virology; D007181:Inclusion Bodies, Viral / Q000235:genetics / Q000648:ultrastructure; D008854:Microscopy, Electron; D030381:Nodaviridae / Q000235:genetics / Q000472:pathogenicity* / Q000648:ultrastructure; D016366:Open Reading Frames; D010473:Perciformes / Q000821:virology; D012367:RNA, Viral / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000648:ultrastructure
D036022:Capsid Proteins; D004279:DNA, Viral; D012367:RNA, Viral; D011994:Recombinant Proteins