A rapid, automated flow cytometric method to measure activated degranulated platelets by density determination.
Platelet activation is reported to correlate with acute coronary syndromes. A platelet analysis method on the ADVIA 120 Hematology System provides rapid analysis of platelet density, reported as mean platelet component (MPC) concentration, utilizes routine hematology specimens, requires no pre-treatment, and thirty seconds to generate results. Sub-populations of platelets separated by density gradients showed excellent correlation with the ADVIA 120 MPC parameter (r = 0.997). Platelet activation induced by thrombin treatment resulted in a shift of platelets into the lowest density fraction (d</=1.068 g/mL) with a corresponding reduction in MPC from 24.7 to 20.6 g/dL, N = 4 subjects (p < 0.004). There was also excellent correlation between expression of CD62P measured by fluorescence flow cytometry and the ADVIA 120 Hematology System MPC values (r = 0.85). These results indicate that the ADVIA 120 MPC parameter may be a useful new test for assessing activated platelets and platelet density.
Chapman
E Sabrinah
ES
Bayer Diagnostics, 511 Benedict Avenue, Tarrytown, New York 10591 USA. sabrinah.chapman.b@bayer.com
Sorette
Martin
M
Hetherington
Edward
E
Zelmanovic
David
D
Kling
Gail
G
Dugailliez
Jacques
J
Pujol-Moix
Nuria
N
Okrongly
David
D
D001331:Automation; D002138:Calibration; D015550:Cell Degranulation; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D019007:P-Selectin / Q000032:analysis; D015539:Platelet Activation; D010979:Platelet Function Tests / Q000295:instrumentation / Q000379:methods; D013917:Thrombin
D019007:P-Selectin; D013917:Thrombin