[HPLC determination of strychnine and brucine in Semen Strychni and its processed products].
OBJECTIVE
This paper reports a HPLC method for determinition of strychnine and brucine in Semen Strychni and its processed products of Jiangxi method and innovated methed.


METHODS
SiO2 was used as the stationary phase, n-hexane-dichloromethane-methanol-ammonia(47.5:47.5:5:0.35) as the mobile phase, with detection wavelength of 254 nm.


RESULTS
The contents of strychnine and brucine in the processed products of Jiangxi are lower.


CONCLUSIONS
This method is accurate, simple and reliable.
Jiang
Yi-hao
YH
Jiangxi College of Traditional Chinese Medicine, Nangchang 330006, Jiangxi, China. JHY.NCDX@163.com
Yang
Wu-liang
WL
Gong
Qian-feng
QF
D002851:Chromatography, High Pressure Liquid; D006358:Hot Temperature; D010946:Plants, Medicinal / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D013331:Strychnine / Q000031:analogs & derivatives* / Q000032:analysis*; D031461:Strychnos nux-vomica / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods
C083806:brucine; D013331:Strychnine