NIM-R7, a novel marker for resting B1 and marginal-zone B lymphocytes, is also expressed on activated T and B cells.
In mice, follicular B cells have been studied in detail, while two other B-cell subpopulations - marginal-zone B and B1 cells - are less well understood. In this work we report the expression pattern of p58, a lymphocyte-activation marker, recognized by rat monoclonal antibody, NIM-R7, and present on the latter two cell subpopulations. Staining with NIM-R7 showed that undisturbed marginal-zone B cells, as well as peritoneal cavity and splenic B1a cells, constitutively expressed p58, whereas follicular B cells and resting T lymphocytes did not. Ontogenic analysis of different compartments showed that p58 did not appear at any stage of development, prior to the development of mature T or B2 lymphocytes. Upon polyclonal stimulation, however, p58 appeared on both T and B2 lymphocytes. Finally, ricin A-conjugated NIM-R7 was able to kill the BCL1 lymphoma without effect on mature resting B2 cells. Therefore, p58 may be a potential target for diagnosis or therapy of B1 and marginal-zone B-cell malignancies.
Manjarrez-Orduño
Nataly
N
Department of Molecular Biomedicine, Centro de Investigación y Estudios Avanzados del I. P. N., México, Instituto Gulbenkian de Ciencias, Oeiras, Portugal.
Parkhouse
R Michael E
RM
Santos-Argumedo
Leopoldo
L
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D016175:B-Lymphocyte Subsets / Q000378:metabolism*; D016923:Cell Death / Q000187:drug effects; D002454:Cell Differentiation / Q000276:immunology; D002478:Cells, Cultured; D000922:Immunotoxins / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000276:immunology*; D016393:Lymphoma, B-Cell / Q000276:immunology; D016399:Lymphoma, T-Cell / Q000276:immunology; D008562:Membrane Glycoproteins / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009363:Neoplasm Proteins / Q000378:metabolism; D010529:Peritoneal Cavity / Q000166:cytology; D012276:Ricin / Q000494:pharmacology; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000922:Immunotoxins; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; D012276:Ricin