title: Low doses of diagnostic energy X-rays protect against neoplastic transformation in vitro.
abstract: OBJECTIVE
  To investigate the effect of low doses of 60 kVp X-rays on in vitro transformation frequency.
  
  
  METHODS
  HeLa x skin fibroblast human hybrid cells were used to assay transformation from the non-tumorigenic to the tumorigenic phenotype. Subconfluent cultures of cells were exposed to a range of doses of 60 kVp X-rays and seeded for assay of transformation after 24 h post-irradiation holding. Experiments were repeated at least three times and the data pooled for analysis. Transformation frequencies were compared with those of sham-irradiated controls.
  
  
  RESULTS
  At doses < 1 cGy, the observed transformation frequencies were significantly less than those seen in unirradiated cells.
  
  
  CONCLUSIONS
  Low doses (< 1 cGy) of 60 kVp X-rays protect HeLa x skin fibroblast human hybrid cells against neoplastic transformation in vitro.
authors.affiliation: Department of Radiation Oncology, College of Medicine, University of California Irvine, 92697, USA. jlredpat@uci.edu
authors.forename: J L
authors.initials: JL
authors.lastname: Redpath
authors.forename: Q
authors.initials: Q
authors.lastname: Lu
authors.forename: X
authors.initials: X
authors.lastname: Lao
authors.forename: S
authors.initials: S
authors.lastname: Molloi
authors.forename: E
authors.initials: E
authors.lastname: Elmore
mesh_terms: D002471:Cell Transformation, Neoplastic / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006367:HeLa Cells; D006801:Humans; D006822:Hybrid Cells / Q000528:radiation effects; D066298:In Vitro Techniques; D009381:Neoplasms, Radiation-Induced / Q000209:etiology; D012307:Risk Factors; D014965:X-Rays