Novel binding and efficient cellular uptake of guanidine-based peptide nucleic acids (GPNA).
Incorporation of a guanidine functional group into the PNA backbone facilitates cellular uptake of PNA into mammalian cells with efficiency comparable to that of the TAT transduction domain. The modified PNA recognizes and binds to the complementary DNA strand in accordance with Watson-Crick recognition rules. However, unlike polypyrimidine PNA which binds to DNA in 2:1 stoichiometry, the modified PNA binds to complementary DNA in a 1:1 ratio to form a highly stable duplex.
Zhou
Peng
P
Department of Chemistry and Center for Light Microscope Imaging and Biotechnology, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15253, USA.
Wang
Miaomiao
M
Du
Lei
L
Fisher
Gregory W
GW
Waggoner
Alan
A
Ly
Danith H
DH
D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D019791:Guanidine / Q000737:chemistry / Q000378:metabolism* / Q000493:pharmacokinetics; D006801:Humans; D020135:Peptide Nucleic Acids / Q000737:chemistry / Q000378:metabolism* / Q000493:pharmacokinetics; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014407:Tumor Cells, Cultured
D020135:Peptide Nucleic Acids; D004247:DNA; D019791:Guanidine