Measurement of aggrecanase-generated interglobular domain catabolites in the medium and extracts of cartilage explants using Western blot analysis.
Hughes
Clare E
CE
University of Cardiff, UK.
Little
Christopher B
CB
Caterson
Bruce
B
D051722:ADAM Proteins; D000071121:ADAMTS4 Protein; D053674:Aggrecans; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015153:Blotting, Western; D002356:Cartilage / Q000737:chemistry* / Q000201:enzymology; D002384:Catalysis / Q000187:drug effects; D017077:Culture Media, Conditioned / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D010450:Endopeptidases / Q000378:metabolism*; D000939:Epitopes / Q000276:immunology; D016326:Extracellular Matrix Proteins; D006025:Glycosaminoglycans / Q000032:analysis*; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D037181:Lectins, C-Type; D008666:Metalloendopeptidases / Q000378:metabolism; D008722:Methods; D009924:Organ Culture Techniques; D010446:Peptide Fragments / Q000032:analysis* / Q000276:immunology; D011348:Procollagen N-Endopeptidase; D017434:Protein Structure, Tertiary; D011509:Proteoglycans / Q000737:chemistry / Q000378:metabolism*; D011817:Rabbits; D012015:Reference Standards; D013045:Species Specificity; D014020:Tissue Extracts / Q000737:chemistry; D014212:Tretinoin / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D053674:Aggrecans; D000911:Antibodies, Monoclonal; D017077:Culture Media, Conditioned; D000939:Epitopes; D016326:Extracellular Matrix Proteins; D006025:Glycosaminoglycans; D007375:Interleukin-1; D037181:Lectins, C-Type; D010446:Peptide Fragments; D011509:Proteoglycans; D014020:Tissue Extracts; D014409:Tumor Necrosis Factor-alpha; D014212:Tretinoin; D010450:Endopeptidases; D051722:ADAM Proteins; D008666:Metalloendopeptidases; D011348:Procollagen N-Endopeptidase; D000071121:ADAMTS4 Protein; C083641:aggrecanase