Saccharomyces cerevisiae 14-3-3 proteins Bmh1 and Bmh2 participate in the process of catabolite inactivation of maltose permease.
In this study we show that Reg1, the regulatory subunit of the Reg1/Glc7 protein phosphatase (PP1) complex, interacts physically with the two yeast members of the 14-3-3 protein family, Bmh1 and Bmh2. By using different fragments of the Reg1 protein we mapped the interaction domain at the N-terminal part of the protein. We also show that Reg1 and yeast 14-3-3 proteins participate actively in the regulation of the glucose-induced degradation of maltose permease (Mal61).
Mayordomo
Isabel
I
Instituto de Biomedicina de Valencia (CSIC), Jaime Roig 11, Spain.
Regelmann
Jochen
J
Horak
Jaroslav
J
Sanz
Pascual
P
D048948:14-3-3 Proteins; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015151:Immunoblotting; D049990:Membrane Transport Modulators; D026901:Membrane Transport Proteins / Q000037:antagonists & inhibitors*; D008957:Models, Genetic; D009004:Monosaccharide Transport Proteins / Q000235:genetics / Q000378:metabolism*; D009841:Oligonucleotides / Q000737:chemistry; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D027981:Symporters / Q000235:genetics / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D020798:Two-Hybrid System Techniques; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism
D048948:14-3-3 Proteins; C075852:BMH1 protein, S cerevisiae; C097164:BMH2 protein, S cerevisiae; D004791:Enzyme Inhibitors; D049990:Membrane Transport Modulators; D026901:Membrane Transport Proteins; D009004:Monosaccharide Transport Proteins; D009841:Oligonucleotides; D029701:Saccharomyces cerevisiae Proteins; D027981:Symporters; C067575:maltose transport system, S cerevisiae; C021534:maltose permease; D014446:Tyrosine 3-Monooxygenase; D001616:beta-Galactosidase