title: Regulation of NGFI-B expression during the ovulatory process.
abstract: NGFI-B is an immediate-early gene that encodes an orphan nuclear receptor. In the rat ovary, the preovulatory surge of LH induces NGFI-B expression in granulosa cells of preovulatory follicles, reaching a peak within 1 h and declining to control levels at 6 h. The LH-stimulated NGFI-B expression is abolished by alpha-amanitin, but superinduced by cycloheximide. Similarly, treatment of human luteinized granulosa cells with LH causes a rapid and transient stimulation of NGFI-B expression. Interestingly, the induction of NGFI-B expression in response to LH stimulation in preovulatory granulosa cells requires signaling through protein kinase Czeta. Furthermore, two other NGFI-B family members, Nurr1 and Nor1, are also rapidly stimulated by LH in granulosa cells of preovulatory follicles through the activation of protein kinase Czeta. The cell-type specific expression and LH induction of NGFI-B suggests a potential role of NGFI-B in the ovulatory process.
authors.affiliation: Hormone Research Center, Chonnam National University, Kwangju 500-757, South Korea.
authors.forename: Jae-Il
authors.initials: JI
authors.lastname: Park
authors.forename: Hyun-Jeong
authors.initials: HJ
authors.lastname: Park
authors.forename: Yu-Il
authors.initials: YI
authors.lastname: Lee
authors.forename: You-Mi
authors.initials: YM
authors.lastname: Seo
authors.forename: Sang-Young
authors.initials: SY
authors.lastname: Chun
mesh_terms: D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D006107:Granulosa Cells / Q000187:drug effects / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D007986:Luteinizing Hormone / Q000378:metabolism / Q000494:pharmacology; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins / Q000235:genetics; D057105:Nuclear Receptor Subfamily 4, Group A, Member 1; D057126:Nuclear Receptor Subfamily 4, Group A, Member 2; D010060:Ovulation / Q000235:genetics* / Q000502:physiology; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D018160:Receptors, Cytoplasmic and Nuclear; D011987:Receptors, Steroid; D011988:Receptors, Thyroid Hormone; D014157:Transcription Factors / Q000235:genetics*; D000099221:Protein Kinase C zeta
chemical_list: D004268:DNA-Binding Proteins; D007986:Luteinizing Hormone; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D057105:Nuclear Receptor Subfamily 4, Group A, Member 1; D057126:Nuclear Receptor Subfamily 4, Group A, Member 2; D011493:Protein Kinase C; D018160:Receptors, Cytoplasmic and Nuclear; D011987:Receptors, Steroid; D011988:Receptors, Thyroid Hormone; D014157:Transcription Factors; D000099221:Protein Kinase C zeta; C539821:NR4A1 protein, human; C539882:NR4A2 protein, human; C490412:NR4A3 protein, human; C539820:Nr4a1 protein, rat; C539884:Nr4a2 protein, rat; C091333:Nr4a3 protein, rat