[Research into the human leucocyte antigen I deletion and local lymphocytes infiltration in laryngeal carcinoma].
OBJECTIVE
To detect the deletion of human leucotyte antigen (HLA) class I antigen expression in laryngeal carcinoma tissue and discuss the relationship between its expression and tumor infiltrating lymphocyte (TIL) cell infiltration or/and malignancy of tumor cell.


METHODS
From Feb 2001 to May 2001 specific mouse antihuman HLA class I monoclonal antibody was combined to examined tissue, 64 samples of laryngeal carcinoma were detected by streptavidin-peroxidase (SP) method immunohistochemistry.


RESULTS
All of 64 cases, 57 were positive HLA class I antigen and 7 were negative, the negative expression rate was 10.9% (7/64). CD3+ and CD8+ T cell infiltrated in HLA class I positive tumor mass were significantely more than those in negative tissue. the malignant degree elevated along with the declination of HLA class I antigen expression. By 3 years follow up, the mortalitywas not statistically significant in both HLA- group and HLA+ group (P > 0.05).


CONCLUSIONS
There is deletion of HLA class I antigen in laryngeal tissue, which favours penetration of cytotoxic lymphoid cells into tumor mass; alteration in HLA class I expression may be used by cancer cells to avoid immune surveillance.
Niu
Chunbo
C
Department of Otorhinolaryngology Head & Neck Surgery, First Hospital Affiliated to Jilin University, Changchun 130021, China. niuchunbo@hotmail.com
Liu
Xinmei
X
Zhou
Weiguo
W
Jing
Ling
L
Du
Baodong
B
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D005260:Female; D005500:Follow-Up Studies; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology*; D008297:Male; D008875:Middle Aged
D015395:Histocompatibility Antigens Class I