title: A bleaching herbicidal activity of methoxyhygromycin (MHM) produced by an actinomycete strain Streptomyces sp. 8E-12.
abstract: OBJECTIVE
  To screen bioherbicidal isolates and evaluate herbicidal activity of methoxyhygromycin (MHM) produced by Streptomyces sp. 8E-12.
  
  
  RESULTS
  Streptomyces sp. 8E-12 with herbicidal activity was selected through seed germination bioassay. An active metabolite, MHM was isolated from culture broth by carbon absorption, butanol extraction, silica gel, Sephadex LH-20 and G-10 chromatography, and preparative HPLC. The metabolite was identified by electrospray ionization mass spectra (ESI-MS) and 1H- and 13C-NMR spectral data analyses. In vivo herbicidal activity was examined against weeds and crops grown on pots.
  
  
  CONCLUSIONS
  Streptomyces sp. 8E-12 produced a selective herbicidal metabolite which was identified as MHM. The metabolite showed stronger in vivo activity against monocotyledonous plants than dicotyledonous plants, and caused a bleaching (albino symptom) on some weeds including Digitaria sanguinalis and Echinochloa crus-galli.
  
  
  CONCLUSIONS
  These results showed that Streptomyces sp. 8E-12 produced a bioherbicidal metabolite, MHM and can be developed as a biocontrol agent (BCA) for weed control.
authors.affiliation: Biomaterial Research Division, Korea Research Institute of Bioscience and Biotechnology, Taejon 305-600, Korea.
authors.forename: H B
authors.initials: HB
authors.lastname: Lee
authors.forename: C-J
authors.initials: CJ
authors.lastname: Kim
authors.forename: J-S
authors.initials: JS
authors.lastname: Kim
authors.forename: K-S
authors.initials: KS
authors.lastname: Hong
authors.forename: K Y
authors.initials: KY
authors.lastname: Cho
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002934:Cinnamates; D031746:Digitaria / Q000187:drug effects*; D031747:Echinochloa / Q000187:drug effects*; D006540:Herbicides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D006921:Hygromycin B / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D010572:Pest Control, Biological; D021241:Spectrometry, Mass, Electrospray Ionization; D013302:Streptomyces / Q000145:classification / Q000254:growth & development / Q000378:metabolism*
chemical_list: D002934:Cinnamates; D006540:Herbicides; D006921:Hygromycin B; C026273:hygromycin A