Simultaneous determination of dopamine and serotonin on a glassy carbon electrode coated with a film of carbon nanotubes.
A chemically modified electrode based on the carbon nanotube film-coated glassy carbon electrode (GCE) is described for the simultaneous determination of dopamine (DA) and serotonin (5-HT). The multiwall carbon nanotube (MWNT) film-coated GCE exhibits a marked enhancement effect on the current response of DA and 5-HT and lowers oxidation overpotentials. The responses of DA and 5-HT merge into a large peak at a bare GCE, but they yield two well-defined oxidation peaks at the MWNT film-coated GCE. The experimental parameters were optimized, and a direct electrochemical method for the simultaneous determination of DA and 5-HT was proposed. The interference of ascorbic acid (AA) was investigated, and the results showed that a large excess of AA did not interfere with the voltammetric responses of DA and 5-HT. The modified electrode has been successfully applied for the assay of 5-HT and DA in human blood serum.
Wu
Kangbing
K
Department of Chemistry, Wuhan University, Wuhan 430072, China.
Fei
Junjie
J
Hu
Shengshui
S
D000327:Adsorption; D001205:Ascorbic Acid; D002244:Carbon / Q000737:chemistry; D004298:Dopamine / Q000032:analysis* / Q000097:blood; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D006801:Humans; D006863:Hydrogen-Ion Concentration; D036103:Nanotechnology; D037742:Nanotubes, Carbon; D010755:Organophosphates; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D012701:Serotonin / Q000032:analysis* / Q000097:blood; D014527:Uric Acid
D037742:Nanotubes, Carbon; D010755:Organophosphates; D014527:Uric Acid; C002290:dicetylphosphate; D012701:Serotonin; D002244:Carbon; D001205:Ascorbic Acid; D004298:Dopamine