[Effect of congsheng capsule on intracellular calcium concentration in mice after ischemic cerebral injury].
OBJECTIVE
To investigate the effect of Congsheng Capsule (CSC) in intracellular Ca2+ concentration in cerebral intra-synaptosomes of ischemia/reperfusion mice and analogous ischemic injured neurons to estimate the mechanism of CSC in antagonizing ischemic cerebral injury.


METHODS
The concentration of Ca2+ in cerebral intra-synaptosomes of mice and in neurocyte suspension of fetal mice were determined using Fura-2/AM double wavelength fluorescence spectrophotometer.


RESULTS
CSC 3 g/kg and 9 g/kg through gastrogavage could relieve the degree of calcium overloading of intra-synaptosomes in mice after cerebral ischemia/reperfusion. The CSC containing serum could inhibit the elevation of Ca2+ content in neurocytes induced by 1 hour hypoxia and glutamate of high concentration (200 mumol/L).


CONCLUSIONS
CSC plays a role in antagonizing ischemic injury through relieving the calcium overloading after cerebral ischemia.
Zhao
Ling
L
Department of Pharmacology, Xuanwu Hospital, Capital University of Medical Sciences, Beijing Key Laboratory for Brain Aging, Beijing 100053.
Xu
Qiu-ping
QP
Li
Lin
L
D000818:Animals; D001921:Brain / Q000378:metabolism; D002545:Brain Ischemia / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000627:therapeutic use*; D002214:Capsules; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005260:Female; D007424:Intracellular Fluid / Q000378:metabolism; D008297:Male; D051379:Mice; D018696:Neuroprotective Agents / Q000627:therapeutic use; D008517:Phytotherapy; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism; D013574:Synaptosomes / Q000378:metabolism
D002121:Calcium Channel Blockers; D002214:Capsules; D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D002118:Calcium