Effect of alloxan and insulin immunoneutralization on circulating thyroid hormone levels in larval landlocked sea lampreys, petromyzon marinus.
The effects of alloxan, an insulin (INS)-secreting cell toxin, and INS immunoneutralization on circulating levels of thyroid hormones (thyroxine, T(4); triiodothyronine, T(3)) were examined in larval landlocked sea lampreys, Petromyzon marinus. Animals were injected intraperitoneally with either (Experiment 1) saline (0.6%) or alloxan (20 or 200 microg/g body weight), or with (Experiment 2) normal rabbit serum or anti-lamprey INS. Alloxan (200 microg/g) decreased plasma T(3), but not T(4), in larvae by about 45-80%. Three, six, or nine hr after acute immunoneutralization of lamprey INS with anti-lamprey INS, plasma T(3) levels decreased by 13-30%, relative to controls. These data indicate that INS deficiency can regulate the thyroid system of larval lampreys. There is some suggestion that INS may mediate the metamorphic processes by modulating thyroid hormone concentrations.
Kao
Yung-Hsi
YH
Department of Life Science, College of Science, National Central University, Taoyuan, Taiwan.
Manzon
Richard G
RG
Sheridan
Mark A
MA
Leatherland
John F
JF
Holmes
John A
JA
Youson
John H
JH
D000496:Alloxan / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D007274:Injections, Intraperitoneal; D007328:Insulin / Q000008:administration & dosage / Q000494:pharmacology*; D007798:Lampreys / Q000097:blood* / Q000254:growth & development; D007814:Larva; D008675:Metamorphosis, Biological; D013963:Thyroid Hormones / Q000097:blood / Q000378:metabolism*; D013974:Thyroxine / Q000097:blood / Q000187:drug effects; D014284:Triiodothyronine / Q000097:blood / Q000187:drug effects
D007328:Insulin; D013963:Thyroid Hormones; D014284:Triiodothyronine; D000496:Alloxan; D013974:Thyroxine