Determination of organophosphorus aromatic nitro insecticides and p-nitrophenol by microbial-cell respiratory activity.
We examined the possibility of measuring the organophosphorus aromatic nitro insecticides metaphos and sumithion as well as their hydrolysis product p-nitrophenol (PNP) by the specific respiratory activity (SRA) of Pseudomonas putida C-11, P. putida BA-11, and Acinetobacter calcoaceticum A-122. The plots of cellular SRA against the two insecticides and PNP were linear over the ranges of 0.5-2.5 microM for P. putida C-11 and BA-11 and 0.5-1.0 microM for A. calcoaceticum A-122. P. putida BA-11 showed the greatest respiratory-response selectivity in the determination of the test substrates. We made comparison studies of the SRA of cells immobilised by two methods: carrier-surface adsorption and inclusion in various gels. We discuss the feasibility of developing a microbial sensor system for the determination of metaphos, sumithion, and PNP in aqueous media.
Guliy
O I
OI
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, 410015 Saratov, Russian Federation.
Ignatov
O V
OV
Makarov
O E
OE
Ignatov
V V
VV
D016954:Acinetobacter calcoaceticus / Q000737:chemistry / Q000502:physiology*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D018914:Cells, Immobilized; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D005278:Fenitrothion / Q000032:analysis* / Q000378:metabolism; D006841:Hydrocarbons, Aromatic / Q000032:analysis / Q000378:metabolism; D007306:Insecticides / Q000032:analysis / Q000378:metabolism; D008743:Methyl Parathion / Q000032:analysis* / Q000378:metabolism; D017672:Nitrogen Compounds / Q000032:analysis / Q000378:metabolism; D009596:Nitrophenols / Q000032:analysis* / Q000378:metabolism; D010100:Oxygen / Q000032:analysis* / Q000378:metabolism; D016958:Pseudomonas putida / Q000737:chemistry / Q000502:physiology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D006841:Hydrocarbons, Aromatic; D007306:Insecticides; D017672:Nitrogen Compounds; D009596:Nitrophenols; D008743:Methyl Parathion; D010100:Oxygen; D005278:Fenitrothion; C024836:4-nitrophenol