[Expression of CD44v and nm23-H1 proteins in laryngeal carcinoma and its correlation with clinical significance].
OBJECTIVE
To investigate the correlation between the expression of two gene proteins and their clinical significance in laryngeal carcinoma.


METHODS
Sixty fresh laryngeal carcinoma samples were analyzed and 20 para-carcinoma tissue and 12 normal laryngeal mucosa samples were studied as controls. All specimens were collected from patients operated on from 1996 to 1998 in the same department (including 52 males and 8 females, age ranged from 41 to 77). The quantitative expression of CD44v and nm23-H1 proteins was analyzed by flow cytometry (FCM) and immunofluorescence technique.


RESULTS
1. Higher level expression of CD44v and nm23-H1 proteins was detected in the laryngeal carcinoma specimen than in normal laryngeal mucosa. 2. The expression of CD44v protein was positively correlated with the metastasis, clinical staging and pathological classification; but not correlated with sex, age, tumor size, course of the disease, T classification and category of laryngeal cancer. 3. The expression of nm23-H1 protein was inversely correlated with metastasis and clinical staging while positively correlated with age and tumor size; no significant relations were found between the expression and the sex and course of the disease, T classification and category of laryngeal cancer. 4. There was an inverse correlation between the protein expression of CD44v and nm23-H1.


CONCLUSIONS
CD44v and nm23-H1 probably play an important regulating role in the metastasis of laryngeal carcinoma.
Hu
J
J
Department of Otorhinolaryngology, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011.
Zhao
R
R
Shi
H
H
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D054778:NM23 Nucleoside Diphosphate Kinases; D009701:Nucleoside-Diphosphate Kinase; D015513:Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics
D054778:NM23 Nucleoside Diphosphate Kinases; D015513:Oncogene Proteins; D011506:Proteins; C517938:NME1 protein, human; D009701:Nucleoside-Diphosphate Kinase