Acute audiogenic stress-induced activation of CRH neurons in the hypothalamic paraventricular nucleus and catecholaminergic neurons in the medulla oblongata.
Strong c-fos expression was induced in neuronal cells of several brain nuclei and the auditory cortex by a short duration auditory stimulation (white noise) in rats. By double immunostaining, Fos-immunoreactive cell nuclei appeared in corticotropin-releasing hormone (CRH)-containing neurons in the hypothalamic paraventricular nucleus, but not in CRH neurons elsewhere in the brain including the central nucleus of the amygdala. Among brain catecholaminergic neurons, only cells in the medulla oblongata (in the A1/C1and A2/C2 cell groups) established double immunostaining for Fos and tyrosine hydroxylase. Sound stimulus in rats with unilateral tympanotomy and plugging the airways resulted in side differences of Fos immunoreactivity in neurons of the auditory pathways and the auditory cortex, but the effect was bilateral in hypothalamic and amygdaloid nuclei. The present data provide evidence for the participation of CRH-synthesizing neurons in hypothalamus and medullary catecholaminergic neurons in the central organization of responses to audiogenic stress stimuli.
Helfferich
Frigyes
F
Laboratory of Neuromorphology, Department of Anatomy, Medical Faculty of Semmelweis University, Tüzoltó utca 58, H-1094 Budapest, Hungary.
Palkovits
Miklós
M
D000161:Acoustic Stimulation; D000679:Amygdala / Q000378:metabolism / Q000502:physiology; D000818:Animals; D001306:Auditory Pathways / Q000166:cytology / Q000502:physiology; D002395:Catecholamines / Q000502:physiology*; D003346:Corticotropin-Releasing Hormone / Q000502:physiology*; D016762:Genes, fos / Q000187:drug effects; D007150:Immunohistochemistry; D008297:Male; D008526:Medulla Oblongata / Q000166:cytology / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D009622:Noise / Q000009:adverse effects; D010286:Paraventricular Hypothalamic Nucleus / Q000166:cytology / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013315:Stress, Psychological / Q000503:physiopathology*; D014432:Tympanic Membrane / Q000502:physiology; D014446:Tyrosine 3-Monooxygenase / Q000096:biosynthesis / Q000235:genetics
D002395:Catecholamines; D003346:Corticotropin-Releasing Hormone; D014446:Tyrosine 3-Monooxygenase