Crosslinked collagen/chitosan matrix for artificial livers.
Matrices composed of collagen and chitosan may create an appropriate environment for the regeneration of livers. In this study, we have prepared, characterized and evaluated a new collagen/chitosan matrix (CCM). The CCM was made by using crosslinking agent 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) in N-hydroxysuccinimide (NHS) and a 2-morpholinoethane sulfonic acid (MES) buffer system. The chemical characteristics were evaluated by Fourier-transformed infrared (FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS). The mechanical strength was measured by tensile tests. The platelet deposition and hepatocyte culture experiments show that CCM has excellent blood and cell compatibility. The results suggest that the CCM is a promising candidate matrix for implantable bioartificial livers.
Wang
X H
XH
Department of Materials Science & Engineering, Tsinghua University, Beijing 100084, People's Republic of China.
Li
D P
DP
Wang
W J
WJ
Feng
Q L
QL
Cui
F Z
FZ
Xu
Y X
YX
Song
X H
XH
van der Werf
Mark
M
D000818:Animals; D001672:Biocompatible Materials; D001792:Blood Platelets / Q000378:metabolism; D002448:Cell Adhesion; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000378:metabolism; D048271:Chitosan; D003094:Collagen / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D022781:Hepatocytes / Q000378:metabolism; D007259:Infrared Rays; D008099:Liver / Q000378:metabolism; D019164:Liver, Artificial; D008297:Male; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D009025:Morpholines / Q000737:chemistry; D010973:Platelet Adhesiveness; D011485:Protein Binding; D051381:Rats; D013053:Spectrophotometry; D017550:Spectroscopy, Fourier Transform Infrared; D013451:Sulfonic Acids / Q000494:pharmacology; D014965:X-Rays
D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D009025:Morpholines; D013451:Sulfonic Acids; D002686:Chitin; D003094:Collagen; D048271:Chitosan