Preparation of collagen-based materials for wound dressing.
OBJECTIVE
To describe the methods which were used to develop collagen-based materials for wound dressing.


METHODS
Fresh frozen bovine tendon was treated with 0.05 mol/L acetic acid at pH 3.2 for 48-72 hours, homogenized, filtered, mixed with 8% chondroitin sulphate, for creating a deaerated 1.5%-2.5% collagen solution. The solution was lyophilized in either a pre-frozen or non-pre-frozen mould. The collagen sponge was then cross-linked with 0.25% glutaraldehyde for 24 hours. Three other types of wound dressings were developed using a similar method: collagen membrane with a polyurethane membrane onlay, polyurethane-coated collagen membrane and collagen membrane on gauze.


RESULTS
It was demonstrated that the use of frozen bovine tendon was stable, and that the prepared collagen sponge contained pores of 50-400 microm in diameter.


CONCLUSIONS
Collagen could be used as wound dressing.
Wu
Zhigu
Z
Burn Institute, 304th Hospital, Beijing 100037, China. wuzg@cgw.net.cn
Sheng
Zhiyong
Z
Sun
Tongzhu
T
Geng
Miao
M
Li
Junyou
J
Yao
Yongming
Y
Huang
Zuxiu
Z
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001684:Biological Dressings; D002417:Cattle; D003094:Collagen / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D005612:Freeze Drying; D011140:Polyurethanes
D000596:Amino Acids; D011140:Polyurethanes; D003094:Collagen