[Determination of trigonelline in Trigonella foenum-graecum by HPLC].
OBJECTIVE
A HPLC method is established to determine the content of trigonelline in Trigonella foenum-graecum.


METHODS
The medicinal material was extracted by petholeum ether-ethanol. Asahipak NH2P-50 column was used, mobilephase consisted of acetonitrile-water(75:25) and detection wavelength was set at UV 265 nm.


RESULTS
The standard curve was linear in the range of 3.68-73.60 micrograms.mL-1 with the correlation coefficient of 0.9999. The average recovery rate and RSD were 97.4% and 1.83% (n = 6) respectively.


CONCLUSIONS
It provides scientific indexes for quality control of T. foenum-graecum.
Zhao
Huai-qing
HQ
Shenyang Pharmaceutical University, Pharmaceutical College, Shenyang 110015, Liaoning, China.
Qu
Yan
Y
Wang
Xue-ya
XY
Zhang
Hong-jian
HJ
Li
Fa-mei
FM
Masao
Hattori
H
D000470:Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D012639:Seeds / Q000737:chemistry; D029910:Trigonella / Q000737:chemistry*
D000470:Alkaloids; C009560:trigonelline