[Application of rat tail collagen in patch clamp experiment with vestibular hair cells].
OBJECTIVE
To study the feasibility of application of rat-tail collagen in patch clamp research in vestibular hair cells.


METHODS
The effect of self-made rat-tail collagen on promoting adhesion of vestibular hair cells in whole cell patch clamp experiment was observed.


RESULTS
A seal was hard to be formed when the vestibular hair cells suspended among the external solution without rat-tail collagen. However, when the vestibular hair cells were firmly adhesive to the bottom of the recording chamber with rat-tail collagen, a seal can be formed easily, which fitted to the long-term observation and recording. The effect of rat-tail collagen on adhesive to vestibular hair cells is obvious.


CONCLUSIONS
Rat-tail collagen could facilitate the vestibular hair cells to adhesive with the bottom of the recording chamber, which is helpful for long-term patch clamp research, and the collagen is considered as an optimal adhesive reagent to vestibular hair cells for patch clamp research.
Guo
Changkai
C
Department of Otorhinolaryngology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Kong
Weijia
W
D000818:Animals; D003094:Collagen / Q000494:pharmacology*; D005240:Feasibility Studies; D018069:Hair Cells, Vestibular / Q000166:cytology*; D018408:Patch-Clamp Techniques; D051381:Rats
D003094:Collagen