Characterization of the S-RNase promoters from sweet cherry (Prunus avium L.).
Genomic DNA fragments containing the S(3)-, S(4)-, and S(6)-RNase genes were isolated from the sweet cherry (Prunus avium L.) and sequenced. Comparison of the 5'-flanking sequences of these three S-RNases indicated that a highly conserved region (designated CR) existed just upstream from the putative TATA boxes. We postulate that CR contains cis-regulatory element(s) involved in pistil expression. To examine the activity of the isolated S-RNase promoters of sweet cherry in the pistil, we transiently introduced approximately 650-bp fragments of the S(4)- and S(6)-RNase promoters fused to beta-glucuronidase (GUS) gene into the pistil of the petunia using a particle bombardment technique. Histochemical analysis showed that the 5'-flanking region of each S-RNase was active in the pistil. This suggests that cis-regulatory element(s) for pistil-specific expression may exist(s) within the 650-bp region upstream from the TATA box in the sweet cherry S-RNase promoter.
Ishizaka
Takako
T
JST, Regional Joint Research Project of Yamagata Prefecture, Sagae, Japan.
Nakano
Hideaki
H
Suzuki
Takashi
T
Kitashiba
Hiroyasu
H
D024506:5' Flanking Region; D001483:Base Sequence; D019470:Biolistics; D015723:Gene Library; D017930:Genes, Reporter; D008969:Molecular Sequence Data; D032306:Petunia / Q000235:genetics; D011401:Promoter Regions, Genetic; D027861:Prunus / Q000235:genetics*; D012260:Ribonucleases / Q000235:genetics*; D016415:Sequence Alignment; D017385:Sequence Homology
D012260:Ribonucleases