Interaction of a ferrocenoyl-modified peptide with papain: toward protein-sensitive electrochemical probes.
A new ferrocenoyl tetrapeptide, Fc-Gly-Gly-Tyr-Arg-OH, has been synthesized, which acts as an effective competitive inhibitor to papain, with a K(i) of 9 microM at pH 6.2. The electrochemical potential of the ferrocenoyl moiety is influenced by papain binding, resulting in a small cathodic shift of 30 mV.
Plumb
Kevin
K
Department of Chemistry, University of Saskatchewan, 110 Science Place, Saskatoon, SK S7N 5C9, Canada.
Kraatz
Heinz-Bernhard
HB
D004563:Electrochemistry; D005296:Ferrous Compounds / Q000737:chemistry / Q000378:metabolism*; D000075163:Metallocenes; D010206:Papain / Q000737:chemistry / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry / Q000378:metabolism
D005296:Ferrous Compounds; D000075163:Metallocenes; D010455:Peptides; D011506:Proteins; D010206:Papain; C004998:ferrocene