[Expression and clinical implication of multidrug resistance gene and multidrug resistant-associated protein gene in patients with hypopharyngeal squamous cell carcinoma].
OBJECTIVE
To study the relationship between the expression of MDR-1 mRNA/P-glycoprotein (P-gp). MRP mRNA and the clinical pathological characteristics of the patients with hypopharyngeal squamous cell carcinoma.


METHODS
Reverse transcription polymerase chain reaction(RT-PCR) assay was used to detect the expression of MDR-1 mRNA and MRP mRNA in 25 specimens of the untreated advanced hypopharyngeal carcinoma. Meantime, immunohistochemistry SABC technique was used to test P-gp in 60 tumor specimens.


RESULTS
The positive expression rates of MDR-1mRNA and P-gp in hypopharyngeal carcinoma were 48 percent (12/25) and 38.3 percent(23/60), respectively. There was a correlation between expression of MDR-1 mRNA and its product P-gp in 25 patients using RT-PCR assay(P < 0.05). The positive expression rate of MRP mRNA was 52 percent (13/25), which was significantly related to metastases of cervical lymph node (P < 0.01).


CONCLUSIONS
Forty-eight percent of the untreated patients with hypopharyngeal carcinoma presented positive expression of MDR-1 mRNA, which was correlated with its gene product P-gp. The expression of MRP mRNA was associated with increased tumor dissemination and metastases.
Cai
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X
Department of Otorhinolaryngology, First Affiliated Hospital of Shandong Medical University, Jinan 250012.
Wang
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T
Shi
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D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000096:biosynthesis / Q000235:genetics; D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D019450:Genes, MDR / Q000235:genetics*; D006801:Humans; D007012:Hypopharyngeal Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D008297:Male; D008875:Middle Aged; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000235:genetics*; D009362:Neoplasm Metastasis; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D012333:RNA, Messenger