title: Crystallization and preliminary X-ray characterization of a novel calcium-binding protein AtCBL2 from Arabidopsis thaliana.
abstract: A new family of calcineurin B-like calcium-binding proteins has recently been identified in Arabidopsis thaliana. AtCBL2, a member of this family, has been crystallized in the presence of calcium ions using polyethylene glycol as a precipitant at 293 K. The crystals belong to space group C222(1), with unit-cell parameters a = 83.9, b = 118.1, c = 49.1 A. The asymmetric unit contains one molecule, with a V(M) of 2.36 A(3) Da(-1) and a solvent content of 48%. Native diffraction data to 2.1 A resolution have been collected using synchrotron radiation at SPring-8.
authors.affiliation: Graduate School of Integrated Science, Yokohama City University, 1-7-29 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
authors.forename: Masamichi
authors.initials: M
authors.lastname: Nagae
authors.forename: Akira
authors.initials: A
authors.lastname: Nozawa
authors.forename: Nozomu
authors.initials: N
authors.lastname: Koizumi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sano
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hashimoto
authors.forename: Mamoru
authors.initials: M
authors.lastname: Sato
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Shimizu
mesh_terms: D017360:Arabidopsis / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D002135:Calcium-Binding Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014961:X-Ray Diffraction
chemical_list: D029681:Arabidopsis Proteins; D002135:Calcium-Binding Proteins; D011994:Recombinant Proteins; C480758:At5g55990 protein, Arabidopsis