title: Standardization of bovine macrophage monolayers and isolation and culture of trypanosomes.
abstract: We describe a method for culturing over 90% pure bovine macrophages from peripheral blood mononuclear cells separated with Nycoprep. The cells were cultured for 12 days and then stained with esterase and with anti CD14 to test for purity. The method is reproducible and ensures an adequate number of cells for immunological research. Additionally, we report the unexpected finding of Trypanosoma trypomastigotes in our macrophage cultures from bovines belonging to a geographic area from which no bovine trypanosomes had been reported before.
authors.affiliation: Grupo de Inmunovirologia-Biogenesis, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia.
authors.forename: Omar A
authors.initials: OA
authors.lastname: Saldarriaga
authors.forename: Jaime I
authors.initials: JI
authors.lastname: Veláquez
authors.forename: Jorge E
authors.initials: JE
authors.lastname: Ossa
authors.forename: Maria T
authors.initials: MT
authors.lastname: Rugeles
mesh_terms: D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods / Q000592:standards*; D008264:Macrophages / Q000469:parasitology*; D014345:Trypanosoma / Q000302:isolation & purification*