title: Growth and cellular differentiation: a physico-biochemical conundrum? The example of the hand.
abstract: Currently, the predominant hypothesis explains cellular differentiation as an essentially genetic intracellular process. The goal of this paper is to suggest that cell growth and differentiation may be, simply, the result of physical and chemical constraints. Bone growth occurs at the level of cartilage conjunction (growth plate) in a zone of lesser constrain. It appears that this growth also induces muscle, tendon, nerve and skin elongation. This cartilage growth by itself seems to explain the elongation of the hand. Growth stops at puberty likely because of feed-back from an increasing muscle load. The ossification (that is differentiation of cartilage into bone) appears to result from the shear stress induced. The study of bone age, obtained by X-ray picture of the hand, shows that ossification of epiphyses is very precise both in time and space. Computer modelization suggests that this ossification occurs where shear stress is greatest. The cartilage which does not ossify (joint, nose, larynx, ear, bronchus, etc.) is not exposed to high shear. Shear stress induces the secretion of extracellular matrix and a change of the biochemical environment of the cell. Precipitation of calcium phosphate, as in ossification, seems related to the alkalosis induced by shear stress. To speak in more general terms, loss of cellular differentiation, as occurs with cancer, can result from a change in the physical-chemical environments.
authors.affiliation: Service de Radiotherapie, Hôpital Pitié-Salpétrière, Boulevard de l'Hôpital, Paris Cedex, France. laurent.schwartz@polytechnique.fr
authors.forename: L
authors.initials: L
authors.lastname: Schwartz
authors.forename: H
authors.initials: H
authors.lastname: Maitournam
authors.forename: C
authors.initials: C
authors.lastname: Stolz
authors.forename: J M
authors.initials: JM
authors.lastname: Steayert
authors.forename: M C
authors.initials: MC
authors.lastname: Ho Ba Tho
authors.forename: B
authors.initials: B
authors.lastname: Halphen
mesh_terms: D000471:Alkalosis / Q000378:metabolism; D000818:Animals; D001846:Bone Development; D002118:Calcium / Q000378:metabolism; D002356:Cartilage / Q000502:physiology; D002454:Cell Differentiation; D002455:Cell Division; D005109:Extracellular Matrix / Q000378:metabolism; D006225:Hand / Q000254:growth & development* / Q000502:physiology*; D006801:Humans; D010100:Oxygen / Q000378:metabolism; D013314:Stress, Mechanical
chemical_list: D010100:Oxygen; D002118:Calcium