An androgen receptor in the brain of the green frog Rana esculenta.
An androgen receptor (AR) has been detected in both the hypothalamic and extra-hypothalamic area of the brain of the male of the green frog Rana esculenta. 3H-T binding activity was detected separately in the hypothalamic and extra-hypothalamic areas. Experiments of binding assay showed 3H-T binding activity in the nuclear extract but not in the cytosol of both the hypothalamic and the extra-hypothalamic areas. The androgen binding moiety was not strictly specific for androgens, binding also 17 beta-estradiol, although to a lesser extent. 3H-testosterone binding activity fluctuated in both the hypothalamic and the extra-hypothalamic areas throughout the reproductive cycle, and paralleled androgen plasma levels.
Paolucci
Marina
M
Department of Biological and Environmental Sciences, University of Sannio, Via Port'Arsa, 11 82100, Benevento, Italy. paolucci@unisanno.it
D000728:Androgens / Q000097:blood / Q000378:metabolism; D000818:Animals; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D007031:Hypothalamus / Q000378:metabolism; D008297:Male; D011893:Rana esculenta; D011944:Receptors, Androgen / Q000378:metabolism*; D012098:Reproduction / Q000502:physiology; D012621:Seasons; D013739:Testosterone / Q000097:blood / Q000378:metabolism*
D000728:Androgens; D011944:Receptors, Androgen; D013739:Testosterone