Expression of apoptosis-associated protein RCAS1 in macrophages of histiocytic necrotizing lymphadenitis.
Receptor-binding cancer antigen expressed on SiSo cells (RCAS1), which is recognized by the 22-1-1 monoclonal antibody (MoAb) against human uterine adenocarcinoma cell line SiSo, has been identified on various kinds of cancer cells. RCAS1 appears to be an apoptosis-associated protein that induces apoptosis in activated T-cells and erythroid progenitor cells. We previously demonstrated that monocytes/macrophages express RCAS1. In the present study, we investigated RCAS1 expression by 22-1-1 MoAb in histiocytic necrotizing lymphadenitis (HNL), which is characterized by necrotic lesions consisting of T-cells undergoing apoptosis and macrophages in proliferation. Expression of RCAS1 was analyzed by immunohistochemical staining in 9 cases of HNL and in 9 cases of reactive lymphadenitis used as a control. The ratio of RCAS1+ cells to CD68+ cells (monocytes/macrophages) was significantly higher in the patients with HNL than in the patients with reactive lymphadenitis (P = .0002; paired t test). Our findings suggest that RCAS1 expressed on macrophages may play an important role in the induction of activated T-cell apoptosis in cases of HNL.
Abe
Yasunobu
Y
Department of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. abey@intmed3.med.kyushu-u.ac.jp
Ohshima
Koichi
K
Nakashima
Manabu
M
Hara
Keiichi
K
Matsushima
Takamitsu
T
Choi
Ilseung
I
Nishimura
Junji
J
Kikuchi
Masahiro
M
Nawata
Hajime
H
Watanabe
Takeshi
T
Muta
Koichiro
K
D000293:Adolescent; D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000096:biosynthesis / Q000502:physiology*; D016022:Case-Control Studies; D005260:Female; D020042:Histiocytic Necrotizing Lymphadenitis / Q000209:etiology / Q000276:immunology / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D008199:Lymphadenitis; D008213:Lymphocyte Activation; D008264:Macrophages / Q000737:chemistry / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000473:pathology
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; C120762:EBAG9 protein, human