[Qualitative and quantitative methods of chlorogenic acid in commercial Herba Artemisiae Scopariae].
OBJECTIVE
To develop the identification and assay for chlorogenic acid in commercial Herba Artemisiae Scopariae.


METHODS
TLC method was used for identification with silica gel G plate and butyl acetate-formic acid-water (7:2.5:2.5) upper layer as a developing solvent. Chlorogenic acid in 85% methanolic extract was separated on the ODS column with methonal-3% acetic acid solution (15:85) as mobile phase. The detection wavelength was 327 nm.


RESULTS
The qualitative method is repeatable. Chlorogenic acid in extracts is well separated, relationship of injection amount and peak area is linear (r = 0.9998) within the range of 0.075-0.6 microgram. The average recovery is 100.9% and repeatability is 1.4%. Ten samples purchased from different areas in the country were identified and quantified with the methods.


CONCLUSIONS
The methods and data could be used for quality control.
Song
Xiao-jun
XJ
First People' Hospital of Hangzhou, Hangzhou 310006, Zhejiang, China.
Zhang
Yong-xin
YX
Zhang
Ying
Y
Zhang
Qi-wei
QW
D019306:Artemisia / Q000737:chemistry*; D002726:Chlorogenic Acid / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control
D002726:Chlorogenic Acid