Effect of high-intensity training on exercise-induced gene expression specific to ion homeostasis and metabolism.
Changes in gene expression during recovery from high-intensity, intermittent, one-legged exercise were studied before and after 5.5 wk of training. Genes related to metabolism, as well as Na+, K+, and pH homeostasis, were selected for analyses. After the same work was performed before and after the training period, several muscle biopsies were obtained from vastus lateralis muscle. In the untrained state, the Na+-K+-ATPase alpha1-subunit mRNA level was approximately threefold higher (P < 0.01) at 0, 1, and 3 h after exercise, relative to the preexercise resting level. After 3-5 h of recovery in the untrained state, pyruvate dehydrogenase kinase 4 and hexokinase II mRNA levels were elevated 13-fold (P < 0.001) and 6-fold (P < 0.01), respectively. However, after the training period, only pyruvate dehydrogenase kinase 4 mRNA levels were elevated (P < 0.05) during the recovery period. No changes in resting mRNA levels were observed as a result of training. In conclusion, cellular adaptations to high-intensity exercise training may, in part, be induced by transcriptional regulation. After training, the transcriptional response to an exercise bout at a given workload is diminished.
Nordsborg
Nikolai
N
Institute of Exercise and Sports Sciences, August Krogh Institute, 2100 Copenhagen, Denmark.
Bangsbo
Jens
J
Pilegaard
Henriette
H
D018528:ATP-Binding Cassette Transporters; D000328:Adult; D017931:DNA Primers; D015444:Exercise / Q000502:physiology*; D005786:Gene Expression Regulation / Q000235:genetics / Q000502:physiology*; D006706:Homeostasis / Q000235:genetics*; D006801:Humans; D007477:Ions / Q000378:metabolism; D054086:KATP Channels; D008297:Male; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D025221:Phosphofructokinases / Q000096:biosynthesis / Q000235:genetics; D010809:Physical Fitness / Q000502:physiology*; D015221:Potassium Channels / Q000096:biosynthesis / Q000235:genetics; D024661:Potassium Channels, Inwardly Rectifying; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016914:Ribonuclease H / Q000096:biosynthesis / Q000235:genetics; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D018528:ATP-Binding Cassette Transporters; D017931:DNA Primers; D007477:Ions; D054086:KATP Channels; D015221:Potassium Channels; D024661:Potassium Channels, Inwardly Rectifying; D012333:RNA, Messenger; C092520:uK-ATP-1 potassium channel; D025221:Phosphofructokinases; D016914:Ribonuclease H; D000254:Sodium-Potassium-Exchanging ATPase