title: Potassium current properties in apical and basal inner hair cells from guinea-pig cochlea.
abstract: Inner hair cells (IHCs) of guinea-pigs were separately isolated from the apical and basal turn and the potassium currents were measured by the whole-cell voltage-clamp technique. The potassium current flows through two types of membrane conductance: a fast (I(k,f)), tetraethylammonium (TEA)-sensitive conductance and a slow (I(k,s)), TEA-resistant conductance. Membrane conductance demonstrated no significant differences between apical IHCs and basal IHCs. Reversal potentials were -65+/-2 mV and -68+/-5 mV in apical and basal IHCs, respectively. The rate of outward current activation was voltage dependent and faster in basal IHCs than in apical IHCs. TEA effect was stronger on basal IHCs than on apical IHCs, suggesting that I(k,f) is dominant in basal IHCs.
authors.affiliation: Department of Otorhinolaryngology, Miyazaki Medical College, 5200 Kihara, Kiyotake-cho, Miyazaki 889-1692, Japan. kimituki@fc.miyazaki-med.ac.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Kimitsuki
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kawano
authors.forename: Keiji
authors.initials: K
authors.lastname: Matsuda
authors.forename: Atsushi
authors.initials: A
authors.lastname: Haruta
authors.forename: Takahiro
authors.initials: T
authors.lastname: Nakajima
authors.forename: Shizuo
authors.initials: S
authors.lastname: Komune
mesh_terms: D000818:Animals; D004553:Electric Conductivity; D006168:Guinea Pigs; D006199:Hair Cells, Auditory, Inner / Q000187:drug effects / Q000502:physiology*; D007700:Kinetics; D008564:Membrane Potentials; D018408:Patch-Clamp Techniques; D026902:Potassium Channel Blockers / Q000494:pharmacology; D015221:Potassium Channels / Q000502:physiology*; D011930:Reaction Time; D019789:Tetraethylammonium / Q000494:pharmacology
chemical_list: D026902:Potassium Channel Blockers; D015221:Potassium Channels; D019789:Tetraethylammonium