[Isolation and elucidation of chemical constituents of shandanshaoyao decoction (II)].
OBJECTIVE
To study the chemical constituents of Shandanshaoyao Decoction.


METHODS
The sample was prepared by boiling water, then precipitated by 75% of ethanol. The ethanol soluble fraction was extracted by chloroform, ethyl acetate and n-butanol, respectively. The extracts were isolated using macroreticular resin, silica gel and polyamide column chromatography, and then the chemical constituents were identified by chemical and spectroscopic methods.


RESULTS
Nine compounds were isolated from the n-butanol extract and water soluble fraction, and elucidated as vitexin, quercetin, hyperoside, rutin, paeoniflorin, protocatechaldehyde, (+)-catechin, danshensu and citric acid respectively.


CONCLUSIONS
The chemical constituents of traditional Chinese medicinal preparations Shandanshaoyao Decoction were reported for the first time.
Wen
Z M
ZM
Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050, China.
Xu
L X
LX
D047310:Apigenin; D001565:Benzoates / Q000737:chemistry / Q000302:isolation & purification*; D001952:Bridged-Ring Compounds / Q000737:chemistry / Q000302:isolation & purification*; D027825:Crataegus / Q000737:chemistry*; D004338:Drug Combinations; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification*; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification*; D039821:Monoterpenes; D029596:Paeonia / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011794:Quercetin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification*; D027561:Salvia miltiorrhiza / Q000737:chemistry; D006027:Glycosides
D047310:Apigenin; D001565:Benzoates; D001952:Bridged-Ring Compounds; D004338:Drug Combinations; D005419:Flavonoids; D005960:Glucosides; D039821:Monoterpenes; D011794:Quercetin; C015423:peoniflorin; D006027:Glycosides; C021304:hyperoside; C032731:vitexin