On-chip single-cell microcultivation assay for monitoring environmental effects on isolated cells.
We have developed a on-chip single-cell microcultivation assay as a means of observing the adaptation process of single bacterial cells during nutrient concentration changes. This assay enables the direct observation of single cells captured in microchambers made on thin glass slides and having semipermeable membrane lids, in which cells were kept isolated with optical tweezers. After changing a medium of 0.2% (w/v) glucose concentration to make it nutrient-free 0.9% NaCl medium, the growth of all cells inserted into the medium stopped within 20 min, irrespective of their cell cycles. When a nutrient-rich medium was restored, the cells started to grow again, even after the medium had remained nutrient-free for 42 h. The results indicate that the cell's growth and division are directly related to their nutrient condition. The growth curve also indicates that the cells keep their memory of what their growth and division had been before they stopped growing.
Umehara
Senkei
S
Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro, Tokyo 153-8902, Japan.
Wakamoto
Yuichi
Y
Inoue
Ippei
I
Yasuda
Kenji
K
D000222:Adaptation, Physiological; D001431:Bacteriological Techniques / Q000295:instrumentation / Q000379:methods*; D002455:Cell Division; D003470:Culture Media; D004777:Environment; D004926:Escherichia coli / Q000254:growth & development* / Q000302:isolation & purification / Q000502:physiology; D007700:Kinetics
D003470:Culture Media