[HPLC determination of 6-gingerol in Rhizoma Zingiberis Recens].
OBJECTIVE
To determine the contents of 6-Gingerol in Rhizoma Zingiberis Recens.


METHODS
HPLC method was used, with Alltech C18 column, acetonitrile-methol-water (43:5:52) as mobile phase with a flow rate of 0.8 mL.min-1, detecting wavelength 280 nm, and column temperature 35 degrees C.


RESULTS
Retained time of 6-gingerol was near 19 min, showing a good recovery (98.2%) and linear correlation (r = 0.9999). The contents of 6-gingerol were 1.35-2.87 mg.g-1, and the water contents were 70.4-85.5% mL.g-1 in Rhizoma Zingiberis Recens.


CONCLUSIONS
The method is appropriate for the determination of 6-gingerol in Rhizoma Zingiberis Recens. Gingerol can be used as a chemical marker of the quality control of Rhizoma Zingiberis Recens.
Wang
Wei-hao
WH
Institute of Chinese Meteria Medica, China Academy of Traditional Chinese Medicine, Bejing 100700, China.
Wang
Zhi-min
ZM
Xu
Li-zhen
LZ
Yang
Shi-lin
SL
D002396:Catechols; D002851:Chromatography, High Pressure Liquid; D005233:Fatty Alcohols / Q000032:analysis*; D020939:Zingiber officinale / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D027343:Rhizome / Q000737:chemistry
D002396:Catechols; D005233:Fatty Alcohols; C007845:gingerol