Interactions of the Caulobacter crescentus XerC and XerD recombinases with the E. coli dif site.
In most bacteria, chromosome dimers arise from homologous recombination between replicated chromosomes. These dimers are then resolved by the action of the XerC and XerD recombinases, which act on the chromosomal dif site in the presence of the FtsK cell division protein. We have cloned the xerC and xerD genes from Caulobacter crescentus, and overexpressed them as maltose-binding protein fusion proteins. These fusion proteins were purified and used in in vitro DNA-binding assays to the Escherichia coli dif site with each protein individually, and in combination with each other. In addition, combinations of Xer proteins from E. coli were also tested for cooperativity with the corresponding C. crescentus proteins.
Jouan
Loubna
L
Département de Microbiologie et Immunologie, Université de Montréal, C.P. 6128 Succ. Centre-Ville, Montréal, QC, Canada H3C3J7.
Szatmari
George
G
D016935:Caulobacter crescentus / Q000201:enzymology* / Q000235:genetics; D004254:DNA Nucleotidyltransferases / Q000235:genetics* / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D029968:Escherichia coli Proteins; D019426:Integrases; D008969:Molecular Sequence Data; D045522:Recombinases; D011995:Recombination, Genetic; D017386:Sequence Homology, Amino Acid
D029968:Escherichia coli Proteins; D045522:Recombinases; C473360:XerC protein, E coli; D004254:DNA Nucleotidyltransferases; D019426:Integrases; C473361:XerD protein, E coli