title: [Effect of congsheng capsule on intracellular calcium concentration in mice after ischemic cerebral injury].
abstract: OBJECTIVE
  To investigate the effect of Congsheng Capsule (CSC) in intracellular Ca2+ concentration in cerebral intra-synaptosomes of ischemia/reperfusion mice and analogous ischemic injured neurons to estimate the mechanism of CSC in antagonizing ischemic cerebral injury.
  
  
  METHODS
  The concentration of Ca2+ in cerebral intra-synaptosomes of mice and in neurocyte suspension of fetal mice were determined using Fura-2/AM double wavelength fluorescence spectrophotometer.
  
  
  RESULTS
  CSC 3 g/kg and 9 g/kg through gastrogavage could relieve the degree of calcium overloading of intra-synaptosomes in mice after cerebral ischemia/reperfusion. The CSC containing serum could inhibit the elevation of Ca2+ content in neurocytes induced by 1 hour hypoxia and glutamate of high concentration (200 mumol/L).
  
  
  CONCLUSIONS
  CSC plays a role in antagonizing ischemic injury through relieving the calcium overloading after cerebral ischemia.
authors.affiliation: Department of Pharmacology, Xuanwu Hospital, Capital University of Medical Sciences, Beijing Key Laboratory for Brain Aging, Beijing 100053.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhao
authors.forename: Qiu-ping
authors.initials: QP
authors.lastname: Xu
authors.forename: Lin
authors.initials: L
authors.lastname: Li
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D002545:Brain Ischemia / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000627:therapeutic use*; D002214:Capsules; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005260:Female; D007424:Intracellular Fluid / Q000378:metabolism; D008297:Male; D051379:Mice; D018696:Neuroprotective Agents / Q000627:therapeutic use; D008517:Phytotherapy; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism; D013574:Synaptosomes / Q000378:metabolism
chemical_list: D002121:Calcium Channel Blockers; D002214:Capsules; D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D002118:Calcium