Proliferating cell populations in experimentally-induced hydrocephalus in developing rats.
To examine the fate of proliferating brain cells in hydrocephalus (Hydro), experimental Hydro was induced in neonatal rats by intracisternal injection of kaolin and, 3 weeks later, the rats were injected with bromodeoxyuridine (BrdU). The BrdU (+) cells were immunohistochemically analyzed by using antibodies against neural (nestin), neuronal (NeuN) and glial (GFAP and MBP) markers in the posterior cerebrum. The percentage of nestin expression for the BrdU (+) cells was 8% in control and increased from 17% in the Hydro to 33% in the Hydro at an earlier stage after the shunt procedure, but was restored to 6% in the Hydro at a later stage after the shunt procedure. The percentages of GFAP expression showed a similar tendency to those of nestin expression. The BrdU (+) cells did not express either NeuN or MBP throughout the experiments.
Fukushima
N
N
Department of Anatomy, Shinshu University School of Medicine, Matsumoto, 390-8621 Nagano, Japan.
Yokouchi
K
K
Kawagishi
K
K
Ren
G
G
Higashiyama
F
F
Moriizumi
T
T
D000818:Animals; D000831:Animals, Newborn; D001973:Bromodeoxyuridine; D054022:Cerebrum / Q000473:pathology; D004195:Disease Models, Animal; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006849:Hydrocephalus / Q000139:chemically induced / Q000473:pathology*; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000032:analysis; D007616:Kaolin / Q000008:administration & dosage; D004676:Myelin Basic Protein / Q000032:analysis; D009419:Nerve Tissue Proteins / Q000032:analysis; D064231:Nestin; D009778:Occipital Lobe / Q000473:pathology; D010296:Parietal Lobe / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar
D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; D004676:Myelin Basic Protein; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin; D007616:Kaolin; D001973:Bromodeoxyuridine