Sequences affecting the regulation of solvent production in Clostridium acetobutylicum.
The high solvent phenotype of Clostridium acetobutylicum mutants B and H was complemented by the introduction of a plasmid that contains either an intact or partially-deleted copy of solR, restoring acetone and butanol production to wild-type levels. This demonstrates that the solR open reading frame on pSOLThi is not required to restore solvent levels. The promoter region upstream of alcohol dehydrogense E (adhE) was examined in efforts to identify sites that play major roles in the control of expression. A series of adhE promoter fragments was constructed and the expression of each in acid- and solvent-phases of growth was analyzed using a chloramphenicol acetyl-transferase reporter system. Our results show that a region beyond the 0A box is needed for full induction of the promoter. Additionally, we show that the presence of sequences around a possible processing site designated S2 may have a negative role in the regulation of adhE expression.
Scotcher
Miles C
MC
Department of Biochemistry and Cell Biology, MS 140, Rice University, 6100 Main Street, Houston, TX 77005, USA.
Huang
Ke-xue
KX
Harrison
Mary L
ML
Rudolph
Frederick B
FB
Bennett
George N
GN
D000096:Acetone / Q000378:metabolism*; D000426:Alcohol Dehydrogenase / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D000440:Butanols / Q000378:metabolism*; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D003013:Clostridium / Q000235:genetics* / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005816:Genetic Complementation Test; D007218:Industrial Microbiology; D009154:Mutation; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics*; D012997:Solvents / Q000378:metabolism*; D024363:Transcription Initiation Site
D001426:Bacterial Proteins; D000440:Butanols; D004268:DNA-Binding Proteins; D012097:Repressor Proteins; D012997:Solvents; D000096:Acetone; D000426:Alcohol Dehydrogenase; D015500:Chloramphenicol O-Acetyltransferase