Transgenic rabbit producing human growth hormone in milk.
The gene construct WAP(6xHisThr):hGH containing the entire human growth hormone gene (hGH) under the rat whey acidic protein (WAP) promoter regulating the expression in mammary glands of mammals was prepared. The 5' end of the gene was modified by the addition of a sequence encoding six histidine residues and a sequence recognized by thrombin. The gene construct was introduced by microinjection into the male pronucleus of a fertilized oocyte. The founder male rabbit was obtained with the transgene mapping to chromosome 7. The presence of the growth hormone was confirmed in samples of milk collected during the lactation of F1 generation females. The growth hormone can be easily purified by affinity chromatography and cleavage by thrombin to an active form.
Lipiński
Daniel
D
Department of Biochemistry and Biotechnology, August Cieszkowski Agricultural University of Poznań, ul. Wołyńska 35, 60-637 Poznań, Poland.
Jura
Jolanta
J
Kalak
Robert
R
Pławski
Andrzej
A
Kala
Marta
M
Szalata
Marlena
M
Jarmuz
Małgorzata
M
Korcz
Aleksandra
A
Słomska
Karolina
K
Jura
Jacek
J
Gronek
Piotr
P
Smorag
Zdzisław
Z
Pieńkowski
Marek
M
Słomski
Ryszard
R
D000818:Animals; D030801:Animals, Genetically Modified; D002846:Chromatography, Affinity; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D005819:Genetic Markers; D006639:Histidine / Q000737:chemistry; D019382:Human Growth Hormone / Q000378:metabolism*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008892:Milk / Q000378:metabolism*; D008894:Milk Proteins / Q000235:genetics; D009865:Oocytes / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011817:Rabbits; D051381:Rats; D013917:Thrombin / Q000378:metabolism; D019076:Transgenes
D005819:Genetic Markers; D008894:Milk Proteins; C024471:whey acidic proteins; D019382:Human Growth Hormone; D006639:Histidine; D013917:Thrombin