[Investigation on NB4 cell responses to realgar by cDNA microarray].
OBJECTIVE
To elucidate the molecular mechanism of realgar-induced apoptosis and differentiation of acute promyelocytic leukemia(APL) cell line NB4.


METHODS
The response of NB4 cells to realgar was explored with a cDNA microarray representing 1003 different human genes.


RESULTS
The analysis of gene expression profiles indicated that 9 genes were up-regulated and 37 genes were down-regulated. Among the 9 up-regulated genes, 2 genes were involved in proteasome degradation pathway.


CONCLUSIONS
PSMC2, PSMD1 and ITGB1 genes may play a role in the apoptosis and differentiation of NB4 cells.
Wang
Huai-yu
HY
Department of Hematology, First Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi, China.
Liu
Shan-xi
SX
D000970:Antineoplastic Agents / Q000494:pharmacology*; D001152:Arsenicals / Q000494:pharmacology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D052138:Genes, Neoplasm / Q000235:genetics*; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics* / Q000473:pathology; D008419:Materia Medica / Q000494:pharmacology*; D020411:Oligonucleotide Array Sequence Analysis; D013440:Sulfides / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D001152:Arsenicals; D008419:Materia Medica; D013440:Sulfides; C058317:arsenic disulfide