title: Novel variants of murine serotonin transporter mRNA and the promoter activity of its upstream site.
abstract: Three variants of murine serotonin transporter (5-HTT) mRNA, which consist of a different exon-one (exon 1a, exon 1b or exon 1c) and the same exon-two to exon-five, were identified. The promoter region for each exon 1 (p1a, p1b and p1c, respectively), ligated to pGL-3 enhancer vector, had activities significantly higher than the empty vector in all cell lines tested except p1c in PC-12, whereas the activity of p1c was significantly lower than the others. Effects of the treatment of dibutyryl-cyclic AMP, human interferon-alpha or mouse interferon-gamma have different profiles among COS-7, PC-12, C6 glioma and immortalized rat serotonergic raphe neurons, RN46A. These three promoter regions may play a role in the transcription of the 5-HTT and could offer a model of the regulation of 5-HTT production in humans and further the pathogenesis of depression.
authors.affiliation: Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, 1-1, Rokko-dai, Nada, 657-8501, Kobe, Japan. naosaito@kobe-u.ac.jp
authors.forename: Kazuo
authors.initials: K
authors.lastname: Sakai
authors.forename: Chie
authors.initials: C
authors.lastname: Hasegawa
authors.forename: Mutsumi
authors.initials: M
authors.lastname: Okura
authors.forename: Osamu
authors.initials: O
authors.lastname: Morikawa
authors.forename: Takehiko
authors.initials: T
authors.lastname: Ueyama
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Shirai
authors.forename: Norio
authors.initials: N
authors.lastname: Sakai
authors.forename: Naoaki
authors.initials: N
authors.lastname: Saito
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002352:Carrier Proteins / Q000235:genetics*; D002460:Cell Line; D005091:Exons; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007155:Immunologic Factors / Q000494:pharmacology; D016898:Interferon-alpha / Q000494:pharmacology; D008156:Luciferases / Q000235:genetics; D008562:Membrane Glycoproteins / Q000235:genetics*; D026901:Membrane Transport Proteins; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D050486:Serotonin Plasma Membrane Transport Proteins; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D002352:Carrier Proteins; D007155:Immunologic Factors; D016898:Interferon-alpha; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C493230:SLC6A4 protein, human; D050486:Serotonin Plasma Membrane Transport Proteins; C493231:Slc6a4 protein, mouse; C493232:Slc6a4 protein, rat; D003994:Bucladesine; D008156:Luciferases