Comparative roles of the cell wall and cell membrane in limiting uptake of xenobiotic molecules by Saccharomyces cerevisiae.
Using reversible electropermeabilization of cells and spheroplasts, we show that the cell wall and plasma membrane partly account for bleomycin resistance by acting as two independent barriers. We also report on the presence of a membrane protein that may be responsible for bleomycin internalization and toxicity in Saccharomyces cerevisiae.
Aouida
Mustapha
M
Laboratoire Vectorologie et Transfert de Gènes, UMR 8121 CNRS, Institut Gustave-Roussy, 94805 Villejuif Cédex, France.
Tounekti
Omar
O
Belhadj
Omrane
O
Mir
Lluis M
LM
D000903:Antibiotics, Antineoplastic / Q000493:pharmacokinetics*; D001761:Bleomycin / Q000493:pharmacokinetics*; D002462:Cell Membrane / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000187:drug effects / Q000502:physiology; D002473:Cell Wall / Q000378:metabolism; D025141:Drug Resistance, Fungal; D018274:Electroporation; D005656:Fungal Proteins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*; D013104:Spheroplasts / Q000502:physiology
D000903:Antibiotics, Antineoplastic; D005656:Fungal Proteins; D008565:Membrane Proteins; D001761:Bleomycin