Oxygen activation in a copper-containing amine oxidase.
The process by which molecular oxygen is activated to enable it to function as an electron acceptor in biology is poorly understood. The quinoprotein copper-containing amine oxidase (CuAO) catalyses the conversion of primary amines into aldehydes. As well as copper, the enzyme contains an organic cofactor, 2,4,5-trihydroxyphenylalanine quinone (TPQ). Following the formation of aldehyde, the enzyme is left as the two-electron reduced aminoquinol form. Reoxidation of the enzyme back to the resting state uses molecular oxygen, which is reduced to H(2)O(2) in the process, with the additional release of NH(3). To understand the structural basis of oxygen activation in Escherichia coli CuAO (ECAO), catalytically competent crystals were used to trap catalytic intermediates by exposing then to amine substrate and then freeze-trapping under aerobic and anaerobic conditions. Single-crystal visible microspectrophotometry was used to probe the oxidation state of the quinone in the intermediates, as TPQ exhibits a rich palette of colour changes during catalytic turnover. This review will focus on one of these structures, that of the rate-determining species in the crystal under steady-state conditions. This structure has revealed many details regarding oxygen activation in ECAO, including the site of dioxygen binding, and the proton-transfer pathways involved in H(2)O(2) formation.
Wilmot
C M
CM
Department of Biochemistry, Molecular Biology & Biophysics, University of Minnesota, 6-155 Jackson Hall, 321 Church Street SE, Minneapolis, MN 55455, USA. wilmo004@umn.edu
D006631:Amine Oxidase (Copper-Containing) / Q000737:chemistry* / Q000378:metabolism; D018360:Crystallography, X-Ray / Q000379:methods; D004926:Escherichia coli / Q000201:enzymology*; D008958:Models, Molecular; D010100:Oxygen / Q000378:metabolism*; D011487:Protein Conformation
D006631:Amine Oxidase (Copper-Containing); D010100:Oxygen