Genetic ablation of the tumor suppressor menin causes lethality at mid-gestation with defects in multiple organs.
Patients suffering from multiple endocrine neoplasia type 1 (MEN1) are predisposed to multiple endocrine tumors. The MEN1 gene product, menin, is expressed in many embryonic, as well as adult tissues, and interacts with several proteins in vitro and in vivo. However, the biological function of menin remains largely unknown. Here we show that disruption of the Men1 gene in mice causes embryonic lethality at E11.5-E13.5. The Men1 null mutant embryos appeared smaller in size, frequently with body haemorrhages and oedemas, and a substantial proportion of them showed disclosure of the neural tube. Histological analysis revealed an abnormal development of the nervous system and heart hypotrophy in some Men1 null embryos. Furthermore, Men1 null livers generally displayed an altered organization of the epithelial and hematopoietic compartments associated with enhanced apoptosis. Chimerism analysis of embryos generated by injection of Men1 null ES cells, showed that cells lacking menin do not seem to have a general cell-autonomous defect. However, primary Men1 null embryonic fibroblasts entered senescence earlier than their wild-type counterparts. Despite normal proliferation ability, Men1 null ES cells exhibited a deficiency to form embryoid bodies, suggesting an impaired differentiation capacity in these cells. The present study demonstrates that menin plays an important role in the embryonic development of multiple organs in addition to its proposed role in tumor suppression.
Bertolino
Philippe
P
International Agency for Research on Cancer (IARC), 150 Cours Albert-Thomas, F-69008 Lyon, France.
Radovanovic
Ivan
I
Casse
Huguette
H
Aguzzi
Adriano
A
Wang
Zhao-Qi
ZQ
Zhang
Chang-Xian
CX
D000483:Alleles; D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D002454:Cell Differentiation; D002455:Cell Division; D016922:Cellular Senescence; D005347:Fibroblasts / Q000378:metabolism; D005822:Genetic Vectors; D005838:Genotype; D006321:Heart / Q000196:embryology; D006984:Hypertrophy / Q000473:pathology; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008099:Liver / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D008957:Models, Genetic; D009154:Mutation; D009206:Myocardium / Q000378:metabolism; D009432:Neural Crest / Q000196:embryology; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
C105373:MEN1 protein, human; D011518:Proto-Oncogene Proteins