Expression of the bovine growth hormone alters the root morphology in transgenic tobacco plants.
The bovine growth hormone (bGH) is a natural peptide hormone that controls the differentiation, growth and metabolism, and is produced in the pituitary gland of cows. For the production of bGH from plants, two different bgh clones, of which the pGAbGH1 contaions only mature peptide sequences and the pGAbGH15 contains signal sequences and the first intron, as well as mature peptide sequences, were used. Those bghs under the control of the CaMV 35S promoter and NOS terminator were introduced to tobacco plants via Agrobacterium tumefaciens-mediated transformation. By PCR analyses using bgh and nptII specific primers, 17 and 21 putative transformants were respectively selected from pGAbGH1- and pGAbGH15-transformed tobacco plants. Northern blot analysis showed that the most of the transgenic lines expressed the bgh mRNA. Western blot analysis revealed that the pGAbGH1-transformed tobaccos produced recombinant bGH, but pGAbGH15-transformed ones did not produce the protein. Interestingly, some morphological changes were observed in the roots of transgenic tobacco plants. The transgenic tobacco plants had thick and short roots containing few root hairs in contrast to the non-transformed wild type plants.
Oh
Kyunghee
K
Graduate School of Biotechnology, Korea University, Seoul 136-701, Korea.
Cheon
Ban Yoon
BY
Cho
Sung Hyun
SH
Truong
Hoang Quoc
HQ
Ok
Sung Han
SH
Jeung
Ji Ung
JU
Choi
Jang Won
JW
Shin
Jeong Sheop
JS
D000818:Animals; D002417:Cattle; D005822:Genetic Vectors; D013006:Growth Hormone / Q000235:genetics / Q000494:pharmacology*; D018517:Plant Roots / Q000166:cytology / Q000187:drug effects* / Q000235:genetics; D030821:Plants, Genetically Modified; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism; D014170:Transformation, Genetic
D012333:RNA, Messenger; D013006:Growth Hormone