title: Updated Epstein-Barr virus (EBV) DNA sequence and analysis of a promoter for the BART (CST, BARF0) RNAs of EBV.
abstract: Two sequences required for activity of the Epstein-Barr virus BART RNA promoter in transfection assays have been identified by site-directed mutagenesis. One contains a consensus AP-1 site; the other has some similarity to Ets and Stat consensus binding sites. Candidate sequences were suggested by mapping a region of unmethylated DNA in EBV around the BART promoter followed by in vivo footprinting the promoter in the C666-1 nasopharyngeal carcinoma cell line, which expresses BART RNAs. The data are presented in the context of a revised EBV DNA sequence, known as EBV wt, that is proposed as a future standard sequence for EBV.
authors.affiliation: Ludwig Institute for Cancer Research, Faculty of Medicine, Imperial College, St Mary's Campus, Norfolk Place, London W2 1PG, UK.
authors.forename: Orlando
authors.initials: O
authors.lastname: de Jesus
authors.affiliation: Ludwig Institute for Cancer Research, Faculty of Medicine, Imperial College, St Mary's Campus, Norfolk Place, London W2 1PG, UK.
authors.forename: Paul R
authors.initials: PR
authors.lastname: Smith
authors.affiliation: Ludwig Institute for Cancer Research, Faculty of Medicine, Imperial College, St Mary's Campus, Norfolk Place, London W2 1PG, UK.
authors.forename: Lindsay C
authors.initials: LC
authors.lastname: Spender
authors.affiliation: Ludwig Institute for Cancer Research, Faculty of Medicine, Imperial College, St Mary's Campus, Norfolk Place, London W2 1PG, UK.
authors.forename: Claudio
authors.initials: C
authors.lastname: Elgueta Karstegl
authors.affiliation: Institut für Medizinische Mikrobiologie und Hygiene, Universität Regensburg, D-93053 Regensburg, Germany.
authors.forename: Hans Helmut
authors.initials: HH
authors.lastname: Niller
authors.affiliation: Department of Clinical Oncology, Sir Y.K. Pao Centre for Cancer, The Chinese University of Hong Kong, Shatin, Hong Kong Special Administrative Region.
authors.forename: Dolly
authors.initials: D
authors.lastname: Huang
authors.affiliation: Ludwig Institute for Cancer Research, Faculty of Medicine, Imperial College, St Mary's Campus, Norfolk Place, London W2 1PG, UK.
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Farrell
mesh_terms: D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D016384:Consensus Sequence; D018983:DNA Footprinting; D019175:DNA Methylation; D004279:DNA, Viral / Q000235:genetics*; D020031:Epstein-Barr Virus Infections / Q000821:virology; D005091:Exons; D005814:Genes, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D016297:Mutagenesis, Site-Directed; D009303:Nasopharyngeal Neoplasms / Q000821:virology; D011401:Promoter Regions, Genetic; D012367:RNA, Viral / Q000235:genetics*; D014407:Tumor Cells, Cultured; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D004279:DNA, Viral; C121639:RK-BARF0 protein, Human herpesvirus 4; D012367:RNA, Viral; D014764:Viral Proteins