[The effect of dentin phosphoprotein on inducing mineralization].
OBJECTIVE
To investigate the effect of dentin phosphoprotein (DPP) in inducing dentinal mineralization.


METHODS
Human DPP was combined with EAH-Sepharose 4B beads and its function of inducing mineralization was studied in mineralization system in-vitro. The mineral formed on the surface of the beads was analyzed by scanning electron microscopy (SEM) and the structure was analyzed by X-ray diffraction and plasma emission spectrum.


RESULTS
There was mineral formed on the beads with combined DPP and the mineral was calcium phosphates whose ratio of calcium to phosphate was 1.33. The diffractogram of the formed mineral was more similar to hydroxyapatite than to other calcium phosphates.


CONCLUSIONS
When tightly combined with certain support substance, human DPP can induce mineralization.
Luo
Song-jiang
SJ
Beijing Institute of Dental Research, Beijing Hospital for Stomatology, Capital University of Medical Sciences, Beijing 100050, China.
Li
Yu-jing
YJ
Wan
Ling
L
Su
Ying
Y
D002130:Calcium Phosphates / Q000737:chemistry / Q000378:metabolism; D003804:Dentin / Q000737:chemistry / Q000378:metabolism*; D003810:Dentinogenesis; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008903:Minerals / Q000737:chemistry / Q000378:metabolism*; D010750:Phosphoproteins / Q000737:chemistry / Q000378:metabolism*; D014074:Tooth Calcification; D014961:X-Ray Diffraction
D002130:Calcium Phosphates; D008903:Minerals; D010750:Phosphoproteins; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; C485838:calcium phosphate, monobasic, anhydrous; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous