Enzyme transmission during crossflow filtration of yeast suspensions using gas/liquid two-phase flows.
The optimal conditions for recovery of an enzyme were determined using gas/liquid two-phase flows. When filtering the enzyme-only solution under single-phase flow conditions, severe fouling occurred. This fouling was manifest as a decline in flux to less than 2% of the initial water flux and a decline in protein concentration in the permeate to 30% of its initial value, during a five-hour filtration period. When yeast cells were added under the same experimental conditions, enzyme transmission was maintained at 100% for the five-hour period and the enzyme mass flux was twofold higher. During gas-sparged microfiltration of the enzyme/yeast mixture in a permeate-recycling mode at the same liquid flow rate, gas/liquid slug flow strongly decreased the transmission of the enzyme (70% decrease), even though the permeate flux was improved (140% improvement). As a result, the mass flux of the enzyme was significantly reduced. However, with a bubble flow pattern, the permeate flux was 1.5 times higher and the transmission was maintained at a high level. The enzyme mass flux was then 25% higher when compared to single-phase flow filtration conditions. During diafiltration experiments with a bubble flow pattern, a 13% higher enzyme recovery was achieved.
Mercier-Bonin
Muriel
M
Centre de Bioingénierie Gilbert Durand, UMR INSA-CNRS 5504, UMR INSA-INRA 792, Toulouse, France. mercierb@insa-tlse.fr
Fonade
Christian
C
D001671:Biochemistry / Q000379:methods*; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation / Q000379:methods*; D004798:Enzymes / Q000032:analysis* / Q000737:chemistry; D005374:Filtration; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013997:Time Factors
D004798:Enzymes