[Establishment of a method for classification of HBV genome and it's application].
OBJECTIVE
By using the techniques of PCR and restriction fragment length polymorphism (RFLP), we established a classification method for HBV genome based on viral S gene of asymptomatic HBV carriers (AsC).


METHODS
By using this method we classified HBV DNA of AsC living in Guangzhou, Shenyang, Beijing and Chongqing.


RESULTS
Among the AsC of Guangzhou, genotype B was 32.8%, genotype C 42.7%, mixed genotypes B and C 23.0%, others 1.6%; among the AsC of Chongqing, genotype B was 35.0%, genotype C 40.0%, mixed genotypes B and C 25.0%; among the AsC of Beijing, genotype B was 25.0%, genotype C 50.0%, mixed genotypes B and C 25.0%; among the AsC of Shenyang, genotype B was 11.1%, genotype C 88.9%. The prevalent HBV strains in China are genotype C and genotype B, and genotype C is the main genotype in west China.


CONCLUSIONS
The established method for genotyping is simple and convenient. Using this method, we also classified HBV DNA of the patients with chronic hepatitis B and liver cirrhosis living in Guangzhou, and found that mixed genotypes B and C was in 50.0% of the patients detected, suggesting the mixed infection might lead to a severe damage of the liver tissue.
Zhu
B
B
Department of Infectious Diseases, Nan Fang Hospital, Guangzhou 510515.
Luo
K
K
Hu
Z
Z
D002353:Carrier State / Q000453:epidemiology / Q000821:virology; D002681:China / Q000453:epidemiology; D004279:DNA, Viral / Q000032:analysis; D016679:Genome, Viral; D006515:Hepatitis B virus / Q000145:classification* / Q000235:genetics*; D019694:Hepatitis B, Chronic / Q000453:epidemiology / Q000821:virology; D006801:Humans; D008103:Liver Cirrhosis / Q000453:epidemiology / Q000821:virology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D004279:DNA, Viral