title: Accidental infection of laboratory worker with vaccinia virus.
abstract: We report the accidental needlestick inoculation of a laboratory worker with vaccinia virus. Although the patient had previously been vaccinated against smallpox, severe lesions appeared on the fingers. Western blot and polymerase chain reaction-restriction fragment length polymorphism were used to analyze the virus recovered from the lesions. The vaccinia virus-specific immunoglobulin G levels were measured by enzyme-linked immunosorbent assay. Our study supports the need for vaccination for laboratory workers that routinely handle orthopoxvirus.
authors.affiliation: Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro Brazil. nissin@biof.ufrj.br
authors.forename: Nissin
authors.initials: N
authors.lastname: Moussatché
authors.forename: Mari
authors.initials: M
authors.lastname: Tuyama
authors.forename: Sayuri E M
authors.initials: SE
authors.lastname: Kato
authors.forename: Ana Paula V
authors.initials: AP
authors.lastname: Castro
authors.forename: Brian
authors.initials: B
authors.lastname: Njaine
authors.forename: Regina H
authors.initials: RH
authors.lastname: Peralta
authors.forename: José M
authors.initials: JM
authors.lastname: Peralta
authors.forename: Clarissa R A
authors.initials: CR
authors.lastname: Damaso
authors.forename: Paulo F
authors.initials: PF
authors.lastname: Barroso
mesh_terms: D000328:Adult; D000914:Antibodies, Viral / Q000097:blood; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007757:Laboratory Infection / Q000175:diagnosis / Q000209:etiology* / Q000821:virology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D014615:Vaccinia / Q000175:diagnosis / Q000209:etiology*; D014616:Vaccinia virus / Q000302:isolation & purification
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D007074:Immunoglobulin G