title: Analysis of docosahexaenoic acid biosynthesis in Crypthecodinium cohnii by 13C labelling and desaturase inhibitor experiments.
abstract: The lipids of the heterotrophic microalga Crypthecodinium cohnii contain the omega-3 polyunsaturated fatty acid (PUFA) and docosahexaenoic acid (22:6) to a level of over 30%. The pathway of 22:6 synthesis in C. cohnii is unknown. The ability of C. cohnii to use 13C-labelled externally supplied precursor molecules for 22:6 biosynthesis was tested by 13C NMR analysis. Furthermore, the presence of desaturases (typical for aerobic PUFA synthesis) was studied by the addition of specific desaturase inhibitors in the growth medium. The addition of 1-(13)C acetate or 1-(13)C butyrate in the growth medium resulted in 22:6 with only the odd carbon atoms enriched. Apparently, two-carbon units were used as building blocks for 22:6 synthesis and butyrate was first split into two-carbon units prior to incorporation in 22:6. When 1-(13)C oleic acid was added to the growth medium, 1-(13)C oleic acid was incorporated into the lipids of C. cohnii but was not used as a precursor for the synthesis of 22:6. Specific desaturase inhibitors (norflurazon and propyl gallate) inhibited lipid accumulation in C. cohnii. The fatty acid profile, however, was not altered. In contrast, in the arachidonic acid-producing fungus, Mortierella alpina, these inhibitors not only decreased the lipid content but also altered the fatty acid profile. Our results can be explained by the presence of three tightly regulated separate systems for the fatty acid production by C. cohnii, namely for (1). the biosynthesis of saturated fatty acids, (2). the conversion of saturated fatty acids to monounsaturated fatty acids and (3). the de novo synthesis of 22:6 with desaturases involved.
authors.affiliation: Agrotechnological Research Institute (ATO BV), PO Box 17, 6700 AA Wageningen, The Netherlands.
authors.forename: Martin E
authors.initials: ME
authors.lastname: de Swaaf
authors.forename: Theo C
authors.initials: TC
authors.lastname: de Rijk
authors.forename: Pieter
authors.initials: P
authors.lastname: van der Meer
authors.forename: Gerrit
authors.initials: G
authors.lastname: Eggink
authors.forename: Lolke
authors.initials: L
authors.lastname: Sijtsma
mesh_terms: D000179:Acrylates / Q000494:pharmacology*; D000818:Animals; D002247:Carbon Isotopes; D004141:Dinoflagellida / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D004281:Docosahexaenoic Acids / Q000032:analysis / Q000378:metabolism*; D044943:Fatty Acid Desaturases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007553:Isotope Labeling / Q000379:methods*; D020094:Mortierella / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D011435:Propyl Gallate / Q000494:pharmacology*; D011724:Pyridazines / Q000494:pharmacology*
chemical_list: D000179:Acrylates; D002247:Carbon Isotopes; D011724:Pyridazines; D004281:Docosahexaenoic Acids; D011435:Propyl Gallate; D044943:Fatty Acid Desaturases; C036658:acrylic acid; C008677:norflurazone