[Sodium channels in the apical membrane of human nasal epithelial cells].
OBJECTIVE
To study the physiological properties of sodium channels in the apical membrane of human nasal epithelial cells.


METHODS
Serum-free cultured nasal epithelial cells of human inferior turbinate from obstructive sleep apnea syndrome patients on collagen gel-coated membranes at an air-liquid interface were studied by patch clamp technique.


RESULTS
In cell-attached patches, a typical single channels current was recorded, their conductance was 21.09 pS, the reversal potential was -50.96, and 77.78% of them < -40 mV. Permeability ratio PNa/PK > 5.80. In the presence of 10(-4) mmol/L amiliride in the pipette, the incidence of sodium channels decreased from 26.67% to 5.13%. It revealed that a population of channels were inhibited by amiloride. Ca2+ did not influence the incidence of sodium channels(P > 0.05), there were no obviously association between voltage and the open probability of the channels.


CONCLUSIONS
On Na+ channels in cell attached patches of human nasal epithelial cells, most of channels are amiloride-sensitive and selective Na+ over K+; The channels were not activated by extracellular Ca2+, but the open probability of them were voltage-independence.
Zhang
X
X
Department of Otorhinolaryngology, Third Clinical Hospital of Bethune University of Medical Sciences, Changchun 130031.
Guo
Y
Y
Dong
Z
Z
D000328:Adult; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000502:physiology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009297:Nasal Mucosa / Q000473:pathology*; D018408:Patch-Clamp Techniques; D012913:Snoring / Q000473:pathology* / Q000601:surgery; D015222:Sodium Channels / Q000502:physiology*
D015222:Sodium Channels