title: The correspondence between the labeling patterns of antibody RT97, neurofilaments, microtubule associated protein 1B and tau varies with cell types and development stages of chicken retina.
abstract: The correspondence between the labeling patterns of antibody RT97, neurofilaments (NF-M and NF-H), microtubule associated protein 1B (MAP1B) and tau, were studied in the developing chicken. At embryonic day 3 (E3), intense RT97 immunoreactivity (IR) was found to be localized in cells in the region adjacent to the intraretinal space, which separates the inner and outer layers of the optic cup, and this was sustained at E8. However, this pattern changed dramatically at E12, as the intensities of RT97 IR increased in the inner retinal layer, while the outermost layers showed only weak IR. The adult stage retina showed RT97 IR within the nerve fibers of the ganglion cells, the processes of the amacrine cells and the photoreceptors. Additional immunostainings for NF-M, -H, MAP1B and tau showed that the observed changes in RT97 IRs were due to the different expressions of these proteins at different development stages.
authors.affiliation: Department of Anatomy, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-Gu, Seoul 110-799, South Korea.
authors.forename: Dong Hoon
authors.initials: DH
authors.lastname: Shin
authors.forename: Kun-Sei
authors.initials: KS
authors.lastname: Lee
authors.forename: Eunju
authors.initials: E
authors.lastname: Lee
authors.forename: Sa Sun
authors.initials: SS
authors.lastname: Cho
authors.forename: Jaehyup
authors.initials: J
authors.lastname: Kim
authors.forename: Jong Wan
authors.initials: JW
authors.lastname: Kim
authors.forename: Bum Sun
authors.initials: BS
authors.lastname: Kwon
authors.forename: Hye Won
authors.initials: HW
authors.lastname: Lee
authors.forename: Wang Jae
authors.initials: WJ
authors.lastname: Lee
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000906:Antibodies / Q000276:immunology; D002642:Chick Embryo; D002645:Chickens; D007150:Immunohistochemistry; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D016900:Neurofilament Proteins / Q000145:classification / Q000378:metabolism*; D010766:Phosphorylation; D012160:Retina / Q000166:cytology / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D016875:tau Proteins / Q000378:metabolism*
chemical_list: D000906:Antibodies; D008869:Microtubule-Associated Proteins; D016900:Neurofilament Proteins; C085284:microtubule-associated protein 1B; D016875:tau Proteins