title: A unique variant of streptococcal group O-antigen (C-polysaccharide) that lacks phosphocholine.
abstract: Streptococcus mitis strain SK598, which represents a subgroup of biovar 1, possesses a unique variant of the C-polysaccharide found in the cell wall of all strains of Streptococcus pneumoniae and in some strains of S. mitis. This new variant lacks the choline methyl groups in contrast to the previously characterized forms of C-polysaccharide, which all contain one or two choline residues per repeat. The following structure of the repeating unit of the SK598 polysaccharide was established: where AAT is 2-acetamido-4-amino-2,4,6-trideoxy-d-galactose. This structure is identical to the double choline-substituted form of C-polysaccharide, except that it is substituted with ethanolamine instead of choline. This extends the number of recognized C-polysaccharide variants to four.
authors.affiliation: Clinical Research Centre, Analytical Unit, Karolinska Institute, Huddinge Hospital, Sweden.
authors.forename: Niklas
authors.initials: N
authors.lastname: Bergström
authors.forename: Per-Erik
authors.initials: PE
authors.lastname: Jansson
authors.forename: Mogens
authors.initials: M
authors.lastname: Kilian
authors.forename: Uffe B
authors.initials: UB
authors.lastname: Skov Sørensen
mesh_terms: D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008745:Methylation; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D010767:Phosphorylcholine / Q000737:chemistry* / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry*; D034361:Streptococcus mitis / Q000378:metabolism; D013296:Streptococcus pneumoniae / Q000378:metabolism
chemical_list: D019081:O Antigens; D011134:Polysaccharides; D010767:Phosphorylcholine