Age-related decreases in the response of aquaporin-5 to acetylcholine in rat parotid glands.
Aquaporin-5 (AQP5) is important in salivary fluid secretion in response to cholinergic and adrenergic stimuli in rat parotid glands. We hypothesized that expression and function of AQP5 might change with age. Acetylcholine and epinephrine induced increases in AQP5 levels in the apical plasma membranes of both young adult and senescent rats. The stimulatory effect of acetylcholine, but not that of epinephrine, on AQP5 levels in the apical plasma membranes of the cells decreased markedly during aging. The quinuclidine derivative, SNI-2011, induced a persistent increase in AQP5 levels in the apical plasma membrane in the cells of both these rats. The amounts of M(3)-muscarinic receptor and Gq proteins did not decrease during aging. The age-related alteration in the responsiveness of AQP5 in the cells to these stimuli might account for the concomitant changes in nitric oxide synthase activity. These results suggest that SNI-2011 might have therapeutic benefit for the treatment of age-related xerostomia.
Inoue
N
N
Department of Pharmacology, Tokushima University School of Dentistry, Kuramoto-cho, Tokushima 770-8504, Japan.
Iida
H
H
Yuan
Z
Z
Ishikawa
Y
Y
Ishida
H
H
D000109:Acetylcholine / Q000494:pharmacology*; D000322:Adrenergic Agonists / Q000494:pharmacology; D000375:Aging / Q000378:metabolism*; D000818:Animals; D051402:Aquaporin 5; D020346:Aquaporins / Q000032:analysis / Q000187:drug effects*; D002462:Cell Membrane / Q000187:drug effects; D018678:Cholinergic Agents / Q000494:pharmacology*; D004837:Epinephrine / Q000494:pharmacology; D043802:GTP-Binding Protein alpha Subunits, Gq-G11; D020962:Heterotrimeric GTP-Binding Proteins / Q000032:analysis / Q000187:drug effects; D008297:Male; D008565:Membrane Proteins; D018721:Muscarinic Agonists / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000032:analysis / Q000187:drug effects; D010306:Parotid Gland / Q000187:drug effects* / Q000378:metabolism; D011812:Quinuclidines / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D043587:Receptor, Muscarinic M3; D011976:Receptors, Muscarinic / Q000032:analysis / Q000187:drug effects; D013876:Thiophenes
D000322:Adrenergic Agonists; C496040:Aqp5 protein, rat; D051402:Aquaporin 5; D020346:Aquaporins; D018678:Cholinergic Agents; D008565:Membrane Proteins; D018721:Muscarinic Agonists; D011812:Quinuclidines; D043587:Receptor, Muscarinic M3; D011976:Receptors, Muscarinic; D013876:Thiophenes; D019001:Nitric Oxide Synthase; D043802:GTP-Binding Protein alpha Subunits, Gq-G11; D020962:Heterotrimeric GTP-Binding Proteins; C059240:cevimeline; D000109:Acetylcholine; D004837:Epinephrine