title: A simple PCR/RFLP analysis can differentiate between Candida albicans, Aspergillus niger, and Aspergillus fumigatus.
abstract: The clinical management of immunocompromised patients depends on the rapid identification of infectious agents such as fungal pathogens. The procedure described here for accomplishing this uses a sensitive polymerase chain reaction method, previously reported, combined with restriction-enzyme digestion to distinguish between Candida and Aspergillus species and to classify Aspergillus strains.
authors.affiliation: Department of Haematology, University of Wales College of Medicine, Cardiff CF14 4XN, Wales, UK.
authors.forename: Nilgun
authors.initials: N
authors.lastname: Isik
authors.forename: Lewis
authors.initials: L
authors.lastname: White
authors.forename: Rosemary
authors.initials: R
authors.lastname: Barnes
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Poynton
authors.forename: Ken I
authors.initials: KI
authors.lastname: Mills
mesh_terms: D001232:Aspergillus fumigatus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001234:Aspergillus niger / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001483:Base Sequence; D002176:Candida albicans / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers