Direct determination of benzamides in serum by column-switching high-performance liquid chromatography.
A column-switching high-performance liquid chromatographic method with fluorescence detection was developed for the simultaneous determination of four benzamide-type anti-psychotic drugs: sulpiride, tiapride, sultopride and metoclopramide in human serum. In this method, a TSKgel Super-ODS column was used as an analytical column, and a TSKgel G 2000SW was prepared as a pretreatment column. Under the optimized analytical conditions, four benzamide-type anti-psychotic drugs were eluted within 18 min. The detection limits (S/N = 3) for sulpiride, tiapride, sultopride and metoclopramide are 1 ng/ml, 4 ng/ml, 2 ng/ml and 0.5 ng/ml, respectively. Finally, the method was applied to the determination of sulpiride in human serum samples obtained after a single oral dose of sulpiride.
Chiba
Ryoko
R
Showa Pharmaceutical University, Machida 194-8543, Japan. chiba@ac.shoyaku.ac.jp
Ogasawara
Ayako
A
Kubo
Teppei
T
Yamazaki
Hiroyuki
H
Umino
Masuo
M
Ishizuka
Yoichi
Y
D001549:Benzamides / Q000097:blood*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods
D001549:Benzamides