Germ cell mutagenicity of gamma-ethyl-gamma-phenyl-butyrolactone (EPBL) detected in the CF1 mouse-dominant lethal study.
Female and male CFI mice weighing 25-30 g were given 0, 50, 100 or 200 mg/kg of gamma-ethyl-gamma-phenyl-butyrolactone (EPBL) for 5 days intraperitoneally. In the male-dominant lethal phase, males treated with EPBL were mated with untreated females following a 7-day mating schedule with three consecutive mating events. In the female-dominant lethal phase, females treated with EPBL were caged with untreated males. The above dosages and schedule treatments were used. The incidence of pregnancy of females mated on days 1-7 and 8-14 after males were given 200 mg/kg of EPBL and of females given 200 mg/kg when mated to untreated males was decreased. Upon examining surgically exposed uteri and ovaries of pregnant females during the first phase, on gestation days 13-15, an increased incidence of pre-implantation losses with 200 mg/kg of EPBL and an increased incidence of post-implantation losses with 100 and 200 mg/kg was observed. In addition, an increased frequency of pre- and post-implantation losses was seen in females treated with 200 mg/kg. These results support the conclusion that EPBL is a germ cell mutagen and its effects are more pronounced during the post-meiotic stage.
Chamorro
Germán
G
Laboratorio de Toxicologia Preclinica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, M.D.M. Polanco, P.O. Box 314, C.P. 11551, México D.F., Mexico. gchamorro@prodigy.net.mx
Vega
Fernando
F
Madrigal
Eduardo
E
Mercado
Efraín
E
Salazar
María
M
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000633:toxicity*; D000818:Animals; D003338:Corpus Luteum / Q000033:anatomy & histology / Q000187:drug effects; D010064:Embryo Implantation / Q000187:drug effects; D020964:Embryo Loss / Q000139:chemically induced; D004822:Epididymis / Q000033:anatomy & histology / Q000187:drug effects; D005260:Female; D005854:Germ Cells / Q000187:drug effects*; D008297:Male; D051379:Mice; D009153:Mutagens / Q000633:toxicity*; D009929:Organ Size / Q000187:drug effects; D011247:Pregnancy; D011897:Random Allocation; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects; D013737:Testis / Q000033:anatomy & histology / Q000187:drug effects
D009153:Mutagens; C465476:gamma-ethyl-gamma-phenyl-butyrolactone; D015107:4-Butyrolactone