title: Transcription mapping of human herpesvirus 8 genes encoding viral interferon regulatory factors.
abstract: The human herpesvirus 8 (HHV-8) genome contains four tandemly arranged genes encoding viral interferon regulatory factors (vIRF-1 to 4) located between genes 57 and 58. Transcript mapping techniques were employed to determine the sizes, ends and splicing patterns of mRNAs specified by these genes in HHV-8-infected cell lines untreated or chemically induced into the lytic growth cycle. Depending on the cell line used, vIRF-3 transcription was minimally or not induced (i.e. expressed with latent kinetics), whereas the other vIRFs were inducible (i.e. expressed with lytic kinetics). Each gene possessed its own promoter (or promoters) and polyadenylation sites, and all but vIRF-1 were spliced from two exons. vIRF-1 was transcribed in uninduced and induced cells from a single initiation site preceded by a TATA box, with the possible use of an additional TATA box and initiation site in uninduced cells. In induced cells, vIRF-2 was transcribed from a single major initiation site preceded by a TATA box, and vIRF-4 was expressed from two sites each preceded by a TATA box. Transcripts for these genes were insufficiently abundant in uninduced cells to map the 5'-ends. vIRF-3 lacks an obvious TATA box and exhibited heterogeneous 5'-ends in uninduced and induced cells. These data clarify and extend our understanding of the structure and transcription of the HHV-8 vIRF genes.
authors.affiliation: MRC Virology Unit, Institute of Biological and Life Sciences, University of Glasgow, Church Street, Glasgow G11 5JR, UK.
authors.forename: Charles
authors.initials: C
authors.lastname: Cunningham
authors.affiliation: Division of Virology, Institute of Biological and Life Sciences, University of Glasgow, Church Street, Glasgow G11 5JR, UK.
authors.forename: Suzanne
authors.initials: S
authors.lastname: Barnard
authors.affiliation: Division of Virology, Institute of Biological and Life Sciences, University of Glasgow, Church Street, Glasgow G11 5JR, UK.
authors.forename: David J
authors.initials: DJ
authors.lastname: Blackbourn
authors.affiliation: MRC Virology Unit, Institute of Biological and Life Sciences, University of Glasgow, Church Street, Glasgow G11 5JR, UK.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Davison
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D004279:DNA, Viral / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D005814:Genes, Viral; D019288:Herpesvirus 8, Human / Q000235:genetics*; D006801:Humans; D050835:Interferon Regulatory Factors; D008969:Molecular Sequence Data; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D004268:DNA-Binding Proteins; D050835:Interferon Regulatory Factors; D012333:RNA, Messenger; D012367:RNA, Viral; D014157:Transcription Factors; D014764:Viral Proteins; C413486:viral interferon regulatory factors