Uptake of sialic acid by human erythrocyte. Characterization of a transport system.
Upon incubation of human red blood cells (RBC) with [4-9-14C] N-acetylneuraminic acid, the cells incorporated this sugar, as demonstrated by the identification of labelled N-acetylmannosamine in the cytosol, as a result of the action of the sialic acid pyruvate-lyase we discovered previously (Biochimie 84 (2002) 655). The mechanism is saturable and indicates the presence of a limited number of transporter molecules in the RBC membrane. This transport process may have relevance to the desialylation of membrane glycoconjugates which occurs during ageing of erythrocytes.
Bulai
Tatiana
T
Facultatea de Biologie, Laboratorul de Biochimie, Universitatea Alexandru Ioan Cuza, Bd-ul Copou nr.11, 6600 Iasi-6, Romania.
Bratosin
Daniela
D
Artenie
Vlad
V
Montreuil
Jean
J
D001345:Autoradiography; D001692:Biological Transport; D002250:Carbon Radioisotopes; D002855:Chromatography, Thin Layer; D003600:Cytosol / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D027361:Organic Anion Transporters / Q000378:metabolism*; D007652:Oxo-Acid-Lyases / Q000378:metabolism; D012794:Sialic Acids / Q000097:blood / Q000378:metabolism*; D027981:Symporters / Q000378:metabolism*
D002250:Carbon Radioisotopes; D027361:Organic Anion Transporters; D012794:Sialic Acids; D027981:Symporters; C421224:sialic acid transport proteins; D007652:Oxo-Acid-Lyases; C443820:sialate-pyruvate lyase; D019158:N-Acetylneuraminic Acid