Fabrication of assembled virus nanostructures on templates of chemoselective linkers formed by scanning probe nanolithography.
We have developed a multistep route to the fabrication of virus assembled nanostructures with chemoselective protein-to-surface linkers synthesized by an efficient solid-phase method. These linkers were used to create patterns of 30-to-50-nm-width-lines by scanning probe nanolithography. Genetically modified cow pea mosaic virus with unique cysteine residues at specific locations on their capsomers were assembled through covalent linkage on these patterns. The morphology of the assembled structures on these line patterns characterized by atomic force microscopy was found to be strongly influenced by the intervirion interactions.
Cheung
Chin Li
CL
Chemistry and Materials Science Directorate, Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, California 94551, USA.
Camarero
Julio A
JA
Woods
Bruce W
BW
Lin
Tianwei
T
Johnson
John E
JE
De Yoreo
Jim J
JJ
D017798:Comovirus / Q000737:chemistry* / Q000235:genetics; D003432:Cross-Linking Reagents / Q000737:chemistry; D005818:Genetic Engineering; D018625:Microscopy, Atomic Force; D036103:Nanotechnology / Q000379:methods*; D013438:Sulfhydryl Compounds / Q000737:chemistry
D003432:Cross-Linking Reagents; D013438:Sulfhydryl Compounds