title: Time-dependent effects of cycloheximide on long-term memory in the cuttlefish.
abstract: When shown prawns in a glass tube, cuttlefish promptly learn to inhibit their predatory behavior and retain this ability for a long time. The cellular and molecular mechanisms of this long-term memory (LTM) are not yet known. In this study, we analyzed the dependency of LTM on de novo brain protein synthesis. Cycloheximide (CXM), a protein synthesis inhibitor, is injected intravenously immediately, 1 h, 3 h, 4 h or 6 h after the training. Retention is tested 24 h posttraining. The injections of CXM revealed one period of memory sensitivity to pharmacological intervention. CXM administered immediately or 6 h after training has no effect on LTM. Conversely, injections given between 1 and 4 h posttraining resulted in amnesia. Taken together, findings of this study establish for the first time in Sepia officinalis that de novo protein synthesis is an essential and time-dependent event for LTM formation of this form of associative learning.
authors.affiliation: Laboratoire de Psychophysiologie, Université de Caen, Esplanade de la paix, 14032 Caen Cedex, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Agin
authors.forename: Raymond
authors.initials: R
authors.lastname: Chichery
authors.forename: Eric
authors.initials: E
authors.lastname: Maubert
authors.forename: Marie Paule
authors.initials: MP
authors.lastname: Chichery
mesh_terms: D000818:Animals; D001245:Association Learning / Q000187:drug effects; D001362:Avoidance Learning / Q000187:drug effects; D001522:Behavior, Animal / Q000187:drug effects; D003216:Conditioning, Operant / Q000187:drug effects; D003513:Cycloheximide / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005502:Food; D008568:Memory / Q000187:drug effects*; D008974:Mollusca / Q000502:physiology*; D009042:Motivation; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D011500:Protein Synthesis Inhibitors; D003513:Cycloheximide