title: Binding of the CC-chemokine RANTES to syndecan-1 and syndecan-4 expressed on HeLa cells.
abstract: It is believed that proteoglycans influence biological properties of chemokines. We show that the CC chemokine RANTES binds not only to high-affinity binding sites on CCR5-positive HeLa cells but also to low-affinity binding sites on HeLa cells expressing or lacking RANTES G protein-coupled receptors. Coimmunoprecipitation studies demonstrate that RANTES forms complexes with glycanated syndecan (SD)-1 and -4, in addition to CCR5 on the CCR5-positive HeLa cells. Moreover, confocal microscopy analysis shows the colocalization of RANTES with SD-1 and -4. Glycosaminoglycans removal from the cells by glycosaminidases treatment prevented RANTES binding to SD-1 and -4 and decreased RANTES binding to CCR5 on the CCR5-positive HeLa cells. Removal of glycosaminoglycans by glycosaminidases treatment of the complexes, RANTES/SD-1/SD-4/+/-CCR5, immobilized on beads, reversed SD-1 and -4 bindings. Therefore, RANTES bindings to SD-1 and -4 depend on glycosaminoglycans and facilitate RANTES interaction with CCR5. Extracting plasma membrane cholesterol abolished the coimmunoprecipitation of SD-1 with RANTES, suggesting that rafts are involved in RANTES association to SD-1. Confocal microscopy analysis as well as coimmunoprecipitation experiments show a RANTES-independent heteromeric complex on the CCR5-positive HeLa cells, SD-1, SD-4, and CCR5. This complex is likely a functional unit in which proteoglycans may modulate RANTES binding to CCR5.
authors.affiliation: Laboratoire de Biologie Cellulaire, Biothérapies Bénéfices et Risques, UPRES 3410, UFR-SMBH, Université Paris XIII, 74, rue Marcel Cachin, 93017, Bobigny, France.
authors.forename: Hocine
authors.initials: H
authors.lastname: Slimani
authors.forename: Nathalie
authors.initials: N
authors.lastname: Charnaux
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Mbemba
authors.forename: Line
authors.initials: L
authors.lastname: Saffar
authors.forename: Roger
authors.initials: R
authors.lastname: Vassy
authors.forename: Claudio
authors.initials: C
authors.lastname: Vita
authors.forename: Liliane
authors.initials: L
authors.lastname: Gattegno
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D018946:Chemokine CCL5 / Q000378:metabolism*; D003505:Cyclodextrins; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism*; D011233:Precipitin Tests; D011485:Protein Binding; D011509:Proteoglycans / Q000378:metabolism*; D019713:Receptors, CCR5 / Q000378:metabolism; D053668:Syndecan-1; D053671:Syndecan-4; D053667:Syndecans; D047392:beta-Cyclodextrins
chemical_list: D018946:Chemokine CCL5; D003505:Cyclodextrins; D008562:Membrane Glycoproteins; D011509:Proteoglycans; D019713:Receptors, CCR5; C508241:SDC1 protein, human; C508304:SDC4 protein, human; D053668:Syndecan-1; D053671:Syndecan-4; D053667:Syndecans; D047392:beta-Cyclodextrins; C031215:betadex