title: [Qualitative and quantitative methods of chlorogenic acid in commercial Herba Artemisiae Scopariae].
abstract: OBJECTIVE
  To develop the identification and assay for chlorogenic acid in commercial Herba Artemisiae Scopariae.
  
  
  METHODS
  TLC method was used for identification with silica gel G plate and butyl acetate-formic acid-water (7:2.5:2.5) upper layer as a developing solvent. Chlorogenic acid in 85% methanolic extract was separated on the ODS column with methonal-3% acetic acid solution (15:85) as mobile phase. The detection wavelength was 327 nm.
  
  
  RESULTS
  The qualitative method is repeatable. Chlorogenic acid in extracts is well separated, relationship of injection amount and peak area is linear (r = 0.9998) within the range of 0.075-0.6 microgram. The average recovery is 100.9% and repeatability is 1.4%. Ten samples purchased from different areas in the country were identified and quantified with the methods.
  
  
  CONCLUSIONS
  The methods and data could be used for quality control.
authors.affiliation: First People' Hospital of Hangzhou, Hangzhou 310006, Zhejiang, China.
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Song
authors.forename: Yong-xin
authors.initials: YX
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Qi-wei
authors.initials: QW
authors.lastname: Zhang
mesh_terms: D019306:Artemisia / Q000737:chemistry*; D002726:Chlorogenic Acid / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control
chemical_list: D002726:Chlorogenic Acid