title: Ovarian development induced in decapitated female Culex pipiens pallens mosquitoes by infusion of physiological quantities of 20-hydroxyecdysone together with amino acids.
abstract: Infusion of 20-hydroxyecdysone into the hemocoel of unfed decapitated female Culex pipiens pallens mosquitoes, at a very low rate of 500-2000pg per day, often stimulated oögenesis of this species, when the hormone was infused together with amino acids. The hormone alone or amino acids alone showed no such stimulatory effect. Previous reports that using an abdomen ligated immediately after a blood meal for hormone injection reduced the quantity of 20-hydroxyecdysone needed to activate unfed female Aedes aegypti by a few thousand times, are therefore due mainly to a sufficient supply of amino acids from the midgut in the isolated abdomen.
authors.affiliation: Department of aBiology and bChemistry, Juntendo University, Hiraga Gakuendai 1-1, Inba-gun, 270-16, Chiba, Japan
authors.forename: Keikichi
authors.initials: K
authors.lastname: Uchida
authors.forename: Daijiro
authors.initials: D
authors.lastname: Ohmorib
authors.forename: Yuki
authors.initials: Y
authors.lastname: Eshita
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Oda
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kato
authors.forename: Kyoko
authors.initials: K
authors.lastname: Tomizawa
authors.forename: Atsuko
authors.initials: A
authors.lastname: Moribayashi
authors.forename: Akihiro
authors.initials: A
authors.lastname: Fukunaga