5alpha-Reduced androgens block estradiol-BSA-stimulated release of oxytocin.
In this study we test the postulate that estradiol conjugated to bovine serum albumin (E-BSA) acts via receptors for the steroid-binding protein sex hormone binding globulin (SHBG) by attempting to block E-BSA-stimulated release of oxytocin with two antagonists of SHBG receptor actions: the 5alpha-reduced androgens dihydrotestosterone (DHT) and 3alpha-diol. Simultaneous superfusion with either DHT or 3alpha-diol significantly blocked E-BSA-stimulated release of oxytocin. We also found that a wide range of free 17beta-estradiol was unable to stimulate oxytocin release, suggesting that E-BSA stimulates receptors other than those for free estradiol to release oxytocin, perhaps SHBG receptors.
Caldwell
Jack D
JD
Department of Biomedical Sciences, University of Illinois College of Medicine, 1601 Parkview Avenue, 61107-1897, Rockford, IL, USA. jackc@uic.edu
Song
Yan
Y
Englöf
Ila
I
Höfle
Simone
S
Key
Mary
M
Morris
Mariana
M
D015113:Androstane-3,17-diol / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D013196:Dihydrotestosterone / Q000494:pharmacology*; D004347:Drug Interactions; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D010121:Oxytocin / Q000378:metabolism*; D011301:Preoptic Area / Q000187:drug effects* / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000494:pharmacology*
D011956:Receptors, Cell Surface; C043090:estradiol-bovine serum albumin; C063572:sex hormone-binding globulin receptor; D013196:Dihydrotestosterone; D015113:Androstane-3,17-diol; C028895:androstane-3,17-diol glucuronide; D012710:Serum Albumin, Bovine; D004958:Estradiol; D010121:Oxytocin