Effect of 5-methoxypsoralen (5-MOP) on cell apoptosis and cell cycle in human hepatocellular carcinoma cell line.
The chemopreventive role of 5-methoxypsoralen (5-MOP) in the human hepatocellular carcinoma (HCC) cell line was investigated by studying the regulation of proliferation and apoptosis in HCC (J5) cells. Morphological analysis, cell viability assay, DNA analysis and cell-cycle analysis suggest that there are at least three modes of the suppressive effects shown by 5-MOP: (a) kills J5 cells directly; (b) induces apoptosis by arresting J5 cells at the G2/M phase in the cell cycle; (c) induces apoptosis through an independent pathway with cell-cycle arrest at 24-72 h of exposure. Further immunoblot analysis demonstrated that inhibition of cyclin B1 by 5-MOP may play an important role in G2/M arrest of J5 cells and provides an additional way to prevent cells from entering the M phase and undergoing apoptosis. 5-MOP therefore appears to exert its anticarcinogenic properties by cytotoxic effect, inducing apoptosis and inhibiting proliferation in the human hepatocellular carcinoma cell line.
Lee
Y M
YM
Department of Medical Technology, Chungtai Institute of Health Sciences and Technology, Taichung, 406, Republic of, Taiwan, China.
Wu
T H
TH
Chen
S F
SF
Chung
J G
JG
D000078223:5-Methoxypsoralen; D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000473:pathology* / Q000517:prevention & control; D002453:Cell Cycle / Q000187:drug effects*; D018890:Chemoprevention; D019926:Cyclin B / Q000494:pharmacology; D056744:Cyclin B1; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology* / Q000517:prevention & control; D008730:Methoxsalen / Q000031:analogs & derivatives* / Q000494:pharmacology*; D009389:Neovascularization, Pathologic; D014407:Tumor Cells, Cultured
C534699:CCNB1 protein, human; D019926:Cyclin B; D056744:Cyclin B1; D000078223:5-Methoxypsoralen; D008730:Methoxsalen