[Effect of erythromycin on apoptosis of eosinophils in nasal polyps in vitro].
OBJECTIVE
To study the effect of erythromycin on apoptosis of eosinophils in nasal polyps in vitro.


METHODS
Fifteen nasal polyp biopsies were collected during nasal polypectomy. The specimens were cultured with erythromycin in RPMI1640, and cultured without erythromycin as control group. The explants in both groups were taken out at 1, 3, 5 day after culture. The apoptosis of cells was detected by terminal deoxynucleotidyl transferasemediated dUTP nick endlabeling(TUNEL), eosinophils were identified by morphology and serial sections stained with HE, then the apoptosis index (AI) of eosinophils was calculated.


RESULTS
The AI of eosinophils in nasal polyps after cultured for 1,3,5 days with erythromycin were (31.77 +/- 9.52)%, (32.67 +/- 9.44)% and (50.34 +/- 8.78)% respectively. In control group, the AI of eosinophils were (29.18 +/- 7.31)%, (29.82 +/- 12.03)% and (42.25 +/- 8.08)% respectively. There were significant difference between two groups at 5 day after culture(P < 0.05).


CONCLUSIONS
Erythromycin promoted apoptosis of eosinophils in nasal polyps in vitro.
Tang
Jun
J
Department of Otorhinolaryngology, Head and Neck Surgery, Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China. tjent@163.net
Li
Yuan
Y
Xu
Geng
G
Xu
Rui
R
Zhang
Gehua
G
Liu
Xian
X
D000293:Adolescent; D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002648:Child; D004804:Eosinophils / Q000187:drug effects*; D004917:Erythromycin / Q000494:pharmacology*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000473:pathology*
D000900:Anti-Bacterial Agents; D004917:Erythromycin