New structures of the O-specific polysaccharides of Proteus. 3. Polysaccharides containing non-carbohydrate organic acids.
Four new Proteus O-specific polysaccharides were isolated by mild acid degradation from the lipopolysaccharides of P. penneri 28 (1), P. vulgaris O44 (2), P. mirabilis G1 (O3) (3), and P. myxofaciens (4), and their structures were elucidated using NMR spectroscopy and chemical methods. They were found to contain non-carbohydrate organic acids, including ether-linked lactic acid and amide-linked amino acids, and the following structures of the repeating units were established: [Figure: see text], where (S)-Lac and (R)-aLys stand for (S)-1-carboxyethyl (residue of lactic acid) and N(epsilon)-[(R)-1-carboxyethyl]-L-lysine ("alaninolysine"), respectively. The data obtained in this work and earlier serve as the chemical basis for classification of the bacteria Proteus.
Kondakova
A N
AN
Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, 119991, Russia.
Toukach
F V
FV
Senchenkova
S N
SN
Arbatsky
N P
NP
Shashkov
A S
AS
Knirel
Y A
YA
Bartodziejska
B
B
Zych
K
K
Rozalski
A
A
Sidorczyk
Z
Z
D000596:Amino Acids / Q000737:chemistry*; D002240:Carbohydrate Sequence; D019344:Lactic Acid / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D019081:O Antigens / Q000737:chemistry* / Q000302:isolation & purification; D011511:Proteus / Q000737:chemistry* / Q000145:classification*; D011513:Proteus mirabilis / Q000737:chemistry / Q000145:classification; D044095:Proteus penneri / Q000737:chemistry / Q000145:classification; D011514:Proteus vulgaris / Q000737:chemistry / Q000145:classification; D012703:Serotyping
D000596:Amino Acids; D019081:O Antigens; D019344:Lactic Acid