[Studies on the arbutin biosynthesis by hairy root of Panax ginseng C.A. Mayer].
OBJECTIVE
To study basic conditions for biosynthesis arbutin by the hairy root of Panax ginseng.


METHODS
Based on the content of arbutin and the biotransformation rate of hydroquione, these conditions such as the culture stage, the lasting time and the concentration of hydroquione fitting for biosynthesis were found.


RESULTS
After cultured for 22 days, transferred in fresh B5 medium having 2 mmol.L-1 hydroquione, the hairy root biotransformated hydroquione into arbutin in 24 hours, the bioconversion rate was 89.0% and the content of arbutin in dry root was 13.0%.


CONCLUSIONS
Arbutin, was which never found in the genus of Panax ginseng, can be biosynthesized by the hairy root of Panax ginseng.
Zhao
M Q
MQ
Institute of Biotechlogy, China Pharmaceutical University, Nanjing 210038 Jiangsu, China.
Ding
J Y
JY
Liu
J
J
Hu
B
B
D001104:Arbutin / Q000096:biosynthesis* / Q000737:chemistry; D001711:Biotransformation; D046508:Culture Techniques; D015394:Molecular Structure; D005894:Panax / Q000254:growth & development / Q000378:metabolism*; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism; D010946:Plants, Medicinal / Q000254:growth & development / Q000378:metabolism*
D001104:Arbutin