Effect of cadmium and zinc ethanolamine complexes on rat brain monoamine oxidase-B activity in vitro.
Monoamine oxidase-B (MAO-B) from rat brain was inhibited strongly by the prepared cadmium and zinc ethanolamine complexes obtained from their sulphate and chloride salts. The inhibition of MAO-B by these complexes was time-dependent and fully reversible after dilution and sedimentation. In vitro, the cadmium ethanolamine complexes were more potent at inhibiting MAO-B than the zinc complexes. The inhibitory effect of these complexes follow the order: TEA>DEA>MEA, due to the alkyl residues and steric effect properties. The inhibition of MAO-B by cadmium and zinc ethanolamine complexes was a noncompetitive type. The K(i) values were calculated. The influence of the complexes on the activity of MAO-B was rather evaluated. It decreased the MAO-B activity. The IC(50) values of the two potent cadmium and zinc triethanolamine complexes on MAO-B were evaluated indicating that the complexes were tightly binding, but reversible inhibitors for MAO-B. In general, these systems may be used for preventing some neurodegenerative diseases.
Shaban
Nadia Z
NZ
Biochemistry Department, Faculty of Science, Alexandria University, Alexandria, Egypt.
Ali
Alaa E
AE
Masoud
Mamdouh S
MS
D000818:Animals; D001596:Benzylamines / Q000378:metabolism; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D002104:Cadmium / Q000737:chemistry / Q000494:pharmacology*; D002498:Centrifugation / Q000379:methods; D003956:Dialysis; D004795:Enzyme Stability; D019856:Ethanolamine / Q000737:chemistry / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D007700:Kinetics; D008995:Monoamine Oxidase / Q000378:metabolism*; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology*; D009942:Organometallic Compounds / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D013237:Stereoisomerism; D013329:Structure-Activity Relationship; D015032:Zinc / Q000737:chemistry / Q000494:pharmacology*
D001596:Benzylamines; D008996:Monoamine Oxidase Inhibitors; D009942:Organometallic Compounds; D002104:Cadmium; D019856:Ethanolamine; C030796:benzylamine; D008995:Monoamine Oxidase; D015032:Zinc