Population substructures in the soil invertebrate Orchesella cincta, as revealed by microsatellite and TE-AFLP markers.
Microsatellite and three enzyme-amplified fragment length polymorphism (TE-AFLP) DNA markers were used to describe the population genetic structure in the soil dwelling collembolan Orchesella cincta (L.). Two forests were sampled according to a three-level nested hierarchical design, with fixed distances among samples within a parcel and among parcels within a forest. The largest component of variation was found at the smallest scale, within parcels (77-97%), while the smallest component of variation was found between forests. The two different methods to study population structure indicated a similar allocation of variance. Population genetic substructuring was revealed between samples on a scale of 50 m; the degree of substructuring however, varied between parcels and forests. One forest showed a high degree of structure as revealed by microsatellites, while another showed a low degree of structure. A significant deviation from random-mating (average FIS = 0.23) over the two forests was detected. Two of 18 samples showed a difference in population genetic structure between males and females. We discuss the fact that the population genetic structure of O. cincta is significantly affected by long-range dispersal, even though it is a small and wingless insect. This interpretation is supported by observations on tree-climbing behaviour in this species that may facilitate air dispersal. As a consequence, the assumption that migration a priori may be neglected in demographic analysis of O. cincta is incorrect.
Van Der Wurff
A W G
AW
Institute of Ecological Science, Faculty of Earth and Life Sciences, Vrije Universiteit, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands. Andre.vanderWurff@wur.nl
Isaaks
J A
JA
Ernsting
G
G
Van Straalen
N M
NM
D025041:Animal Migration / Q000502:physiology; D000818:Animals; D017753:Ecosystem; D004586:Electrophoresis; D005260:Female; D005787:Gene Frequency; D005828:Genetics, Population; D007313:Insecta / Q000235:genetics* / Q000502:physiology; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D009426:Netherlands; D012150:Polymorphism, Restriction Fragment Length; D012987:Soil; D014197:Trees
D012987:Soil