title: [Somatic hybridization between carrot and Bupleurum scorzonerifolium and esteras isoenzyme analysis].
abstract: OBJECTIVE
  To transfer the effective elements of Bupleurum scorzonerifolium into carrot, and provide theoretical data for the exploitation, improvement and selection of the germplasm of Chinese medicinal plants.
  
  
  METHODS
  The protoplasta of Bupleurum scorzonerifolium irradiated by ultraviolet light (UV) at an intensity of 300 microW.(cm2)-1 for 0, 1, 2 min respectively were fused with those of carrot Fisch by PEG method. The regenerated clones, derived form a single fused cell, were examined for their hybrid nature by phenotype and Esterase isoenzyme analysis.
  
  
  RESULTS
  Nine clones were identified as the somatic hybrids between B. scorzonerifolium and carrot.
  
  
  CONCLUSIONS
  This provides a firm foundation for the further analysis of the main active components saikosaponin of somatic hybrids and the screening out of high-medicine-content hybrid cell lines.
authors.affiliation: Shool of Lifescience, Shandong University, Jinan 250100, Shandong, China.
authors.forename: Xian-rong
authors.initials: XR
authors.lastname: Huang
authors.forename: Feng-ning
authors.initials: FN
authors.lastname: Xiang
authors.forename: Guang-min
authors.initials: GM
authors.lastname: Xia
authors.forename: Da-ying
authors.initials: DY
authors.lastname: Zhi
authors.forename: Jun-ying
authors.initials: JY
authors.lastname: Shi
mesh_terms: D029972:Bupleurum / Q000166:cytology / Q000235:genetics / Q000254:growth & development; D002459:Cell Fusion; D046508:Culture Techniques; D018552:Daucus carota / Q000166:cytology / Q000235:genetics / Q000254:growth & development; D004950:Esterases / Q000032:analysis; D006822:Hybrid Cells / Q000201:enzymology; D006824:Hybridization, Genetic; D007527:Isoenzymes / Q000032:analysis; D010946:Plants, Medicinal / Q000166:cytology / Q000235:genetics / Q000254:growth & development; D011523:Protoplasts / Q000166:cytology
chemical_list: D007527:Isoenzymes; D004950:Esterases