title: [Correlation between prognosis and cell proliferation, apoptosis in squamous cell carcinoma of tongue].
abstract: OBJECTIVE
  To study the correlation between prognosis and cell proliferation, apoptosis in squamous cell carcinoma (SCC) of tongue.
  
  
  METHODS
  Using TdT-mediated dUTP biotin nick end labeling (TUNEL) for cell apoptosis and immunohistochemical staining for proliferating cell nuclear antigen (PCNA), Bcl-2 and Bax protein expression.
  
  
  RESULTS
  In SCC of tongue, the positive rates of apoptosis index (AI), Bcl-2, Bax and PCNA were 55.8%, 65.4%, 73.1% and 100.0% respectively. In the univariate Kaplan-Meier analysis, low expression of Bax, high expression of PCNA, Bcl-2/Bax expression ratio > 1 and high staging were all associated with lower survival rate (Log-rank test, P < 0.05, respectively), AI was only correlated with grading. Bcl-2 did not show a significant association with all features.
  
  
  CONCLUSIONS
  The levels of Bax and PCNA expression and Bcl-2/Bax expression ratio have independent prognosis value in squamous cell carcinoma of the tongue. Comprehensive analysis of these parameters can be helpful to identify biological behavior and predict more accurately the prognosis of patients of tongue squamous cell carcinoma.
authors.affiliation: Department of Pathology, Stomatology Hospital of Guangxi Medical University, Nanning 530021, China.
authors.forename: Yi-ping
authors.initials: YP
authors.lastname: Yang
authors.forename: Hai-yun
authors.initials: HY
authors.lastname: Qing
authors.forename: Yang
authors.initials: Y
authors.lastname: Cao
mesh_terms: D000328:Adult; D000368:Aged; D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D002455:Cell Division; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D011518:Proto-Oncogene Proteins / Q000032:analysis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D016019:Survival Analysis; D015996:Survival Rate; D014062:Tongue Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D051028:bcl-2-Associated X Protein
chemical_list: C494826:BAX protein, human; D018809:Proliferating Cell Nuclear Antigen; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein