Cross-reactivity of HLA-B*1801-restricted T-lymphocyte clones with target cells expressing variants of the human cytomegalovirus 72kDa-IE1 protein.
The impact of natural polymorphism in a cytomegalovirus-dominant HLA-B(*)1801-restricted epitope, IE1(199-206), on the specific responses of T-cell clones was assessed by measuring their cytolytic activity against target cells expressing mutated recombinant IE1 proteins. Our results suggest an in vivo selection of T lymphocytes that cross-react with multiple IE1 variants.
Prod'homme
Virginie
V
Institut National de la Santé et de la Recherche Médicale U463, Institut de Biologie, 44093 Nantes Cedex 1, France.
Retière
Christelle
C
Valtcheva
Ralitza
R
Bonneville
Marc
M
Hallet
Marie-Martine
MM
D000595:Amino Acid Sequence; D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D003429:Cross Reactions; D003587:Cytomegalovirus / Q000276:immunology; D014644:Genetic Variation; D015235:HLA-B Antigens / Q000378:metabolism*; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D016056:Immunodominant Epitopes; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011110:Polymorphism, Genetic; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014764:Viral Proteins
D015235:HLA-B Antigens; C088645:IE1 protein, cytomegalovirus; D017874:Immediate-Early Proteins; D016056:Immunodominant Epitopes; D014764:Viral Proteins