title: Functional characterization of human CD34+ cells that express low or high levels of the membrane antigen CD111 (nectin 1).
abstract: Nectins are recently described adhesion molecules that are widely expressed on many tissues, including the hematopoietic tissue. Nectin 1 (CD111) is expressed on a higher proportion of mobilized peripheral blood (mPB) than cord blood (CB) CD34+ cells, and of CD34+/CD38+ cells when compared with CD34+/CD38- cells. We studied functional properties of human CB and mPB CD34+ cells that express low or high levels of CD111. CD34+/CD111(dim) cells contain a higher proportion of cells in G0/G1 phase than CD34+/CD111(bright) cells. CD34+/CD111(bright) cells contain more erythroid progenitors: CFU-E, than their counterparts, which on the opposite contain more HPP-CFC. Limiting dilution analyses demonstrate a higher frequency of immature progenitors: cobblestone-area colony-forming cells, CD34+/CD111(dim) than in CD34+/CD111(bright) cells. In vitro differentiation assays demonstrate a higher frequency of B-, T- and dendritic-cell precursors, but less NK-cell precursors in CD34+/CD111(dim) cells. Evaluation of engraftment in NOD-SCID mice shows that SCID repopulating cells are more frequent among mPB CD34+/CD111(dim) cells. Liquid culture of CD34+/CD111(dim) cells with erythropoietin shows that CD111 expression increases simultaneously with CD36, following CD71 and before glycophorin A expression. In conclusion, immature human hematopoietic progenitors express low levels of CD111 on their surface. During erythroid differentiation CD34+ cells acquire higher levels of the CD111 antigen.
authors.affiliation: Centre de Thérapie Cellulaire et Génique, Institut Paoli-Calmettes, Centre Régional de Lutte Contre le Cancer Provence-Alpes-Côte d'Azur, 232 boulevard Sainte-Marguerite, 13273 Marseille Cedex 9, France.
authors.forename: G
authors.initials: G
authors.lastname: Belaaloui
authors.forename: A-M
authors.initials: AM
authors.lastname: Imbert
authors.forename: F
authors.initials: F
authors.lastname: Bardin
authors.forename: C
authors.initials: C
authors.lastname: Tonnelle
authors.forename: P
authors.initials: P
authors.lastname: Dubreuil
authors.forename: M
authors.initials: M
authors.lastname: Lopez
authors.forename: C
authors.initials: C
authors.lastname: Chabannon
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism*; D000943:Antigens, Differentiation / Q000378:metabolism; D000954:Antigens, Surface / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002453:Cell Cycle; D002454:Cell Differentiation; D018920:Coculture Techniques; D003114:Colony-Forming Units Assay; D004921:Erythropoietin / Q000378:metabolism; D005312:Fetal Blood / Q000166:cytology; D005434:Flow Cytometry; D006021:Glycophorins / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D000075983:Nectins; D013950:Thymus Gland / Q000166:cytology; D014183:Transplantation, Heterologous
chemical_list: D018952:Antigens, CD34; D000943:Antigens, Differentiation; D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D006021:Glycophorins; C000619010:NECTIN1 protein, human; C000619015:Nectin1 protein, mouse; D000075983:Nectins; D004921:Erythropoietin