title: The role of Ppe1/PP6 phosphatase for equal chromosome segregation in fission yeast kinetochore.
abstract: Mis12 is a kinetochore protein essential for equal chromosome segregation and is evolutionarily conserved from yeast to human. In this study, we report the isolation and characterization of suppressors of the mis12 mutant in fission yeast. Our results indicate that Mis12 is negatively regulated by a highly conserved protein phosphatase Ppe1 (scSit4/dmPPV/hPP6) or its bound partner Ekc1 (scSAP), and it is positively regulated by a counteracting kinase Gsk3. Mass spectrometry analysis shows that at least two sites in Mis12 are phosphorylated. This mechanism of suppression occurs at the level of localization recovery of Mis12 to the kinetochore chromatin. Consistently, Mis12 and a subpopulation of Ppe1/Ekc1 were found to behave like non-histone-type chromatin-associating proteins in the chromatin fractionation assay. Mutant analysis of Ppe1 and Ekc1 revealed that they are important for faithful chromosome segregation, as the mutants exhibited unequal chromosome segregation similar to mis12 in the presence of a low concentration of tubulin poison. Ppe1/PP6 directly or indirectly modulates kinetochore chromatin protein Mis12 to ensure progression into normal anaphase.
authors.affiliation: COE Research Project, Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Kitashirakawa-Oiwakecho, Sakyo-ku, Kyoto 606-8502 Japan.
authors.forename: Gohta
authors.initials: G
authors.lastname: Goshima
authors.forename: Osamu
authors.initials: O
authors.lastname: Iwasaki
authors.forename: Chikashi
authors.initials: C
authors.lastname: Obuse
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Yanagida
mesh_terms: D000595:Amino Acid Sequence; D000705:Anaphase / Q000235:genetics / Q000502:physiology; D018797:Cell Cycle Proteins / Q000235:genetics / Q000502:physiology; D002467:Cell Nucleus / Q000378:metabolism; D002503:Centromere / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D020090:Chromosome Segregation / Q000502:physiology*; D005800:Genes, Fungal; D006801:Humans; D018386:Kinetochores / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D010749:Phosphoprotein Phosphatases / Q000502:physiology*; D010766:Phosphorylation; D011485:Protein Binding; D012568:Schizosaccharomyces / Q000235:genetics* / Q000502:physiology*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D013489:Suppression, Genetic
chemical_list: D018797:Cell Cycle Proteins; D002843:Chromatin; D029702:Schizosaccharomyces pombe Proteins; C492315:mis12 protein, S pombe; C106081:ppe1 protein, S pombe; D010749:Phosphoprotein Phosphatases; C106079:protein phosphatase 6