title: Proton conduction through full-length gp91phox requires histidine 115.
abstract: The NADPH oxidase of neutrophils is a transmembrane electron transfer complex, containing a flavin adenine dinucleotide and two hemes, all of which are suggested to be contained within gp91 (phox), one of four subunits of the enzyme. The transfer of electrons through the NADPH oxidase is associated with an efflux of protons. gp91 (phox) has previously been demonstrated to function as the proton conduction pathway. The mutation of histidines 111, 115, and 119 to leucines and of histidine 115 to leucine within the N-terminal 230-amino-acid fragment of gp91 (phox) has previously been demonstrated to result in the loss of proton conduction through this N-terminal fragment. In this paper we have investigated the role of these histidines in proton conduction by the full-length gp91 (phox). Stable CHO cell lines were established which expressed full-length gp91 (phox) in which histidines 111, 115, and 119 had been mutated to leucines (CHO91H111/115/119) and in which histidine 115 had been mutated to leucine (CHO91H115L). The expression of gp91 (phox) and its cellular localisation in these cell lines were comparable between wild-type and the mutant gp91 (phox). The mutation of histidines 111, 115, and 119 to leucines or just histidine 115 to leucine resulted in an almost total loss of both the arachidonate-activated influx and efflux of protons, in comparison with that observed for wild-type gp91 (phox). Therefore, histidine 115 is required for proton conduction by both full-length gp91 (phox) and the N-terminal 230-amino-acid fragment of gp91 (phox). Histidine 115 has recently been proposed to act as a coordinating ligand for the outer heme iron of the NADPH oxidase. On the basis of observations for cytochrome c oxidase, we propose a model for this dual role of histidine 115.
authors.affiliation: Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol, United Kingdom.
authors.forename: T J
authors.initials: TJ
authors.lastname: Mankelow
authors.forename: L M
authors.initials: LM
authors.lastname: Henderson
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology; D016466:CHO Cells; D006224:Cricetinae; D015870:Gene Expression; D006418:Heme / Q000378:metabolism; D006639:Histidine / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D007501:Iron / Q000378:metabolism; D008024:Ligands; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011522:Protons
chemical_list: D008024:Ligands; D008562:Membrane Glycoproteins; D011522:Protons; D016718:Arachidonic Acid; D006418:Heme; D006639:Histidine; D007501:Iron; C056558:CYBB protein, human; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases