title: Mutations arise independently of transcription in non-dividing bacteria.
abstract: It has been proposed that transcription introduces a bias into the random process of mutation. Although this hypothesis is supported by experimental data for mutations arising during active bacterial growth, the role of transcription in mutagenesis in non-dividing bacteria is entirely hypothetical. In the present study, we tested the hypothesis of a possible role of transcription in a non-dividing E. coli K12 strain. In this strain (BD010), a mutated trpB allele (trpB9578), placed under stringent transcriptional control, was tested for the appearance of prototrophic revertants on synthetic medium lacking tryptophan. The number of phenotypic revertants which appeared in the absence of trp transcription was compared to that observed when the mutated gene was continuously transcribed. Our results showed that transcription of trpB is not mutagenic under conditions of tryptophan starvation, and that the frequency of TrpB+ reversion is solely a function of the duration of starvation.
authors.affiliation: Laboratorio di Microbiologia Generale, Dipartimento di Biologia, Università di Roma Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Barionovi
authors.forename: P
authors.initials: P
authors.lastname: Ghelardini
authors.forename: G
authors.initials: G
authors.lastname: Di Lallo
authors.forename: L
authors.initials: L
authors.lastname: Paolozzi
mesh_terms: D004926:Escherichia coli / Q000235:genetics*; D009154:Mutation; D014158:Transcription, Genetic