[Somatic hybridization between carrot and Bupleurum scorzonerifolium and esteras isoenzyme analysis].
OBJECTIVE
To transfer the effective elements of Bupleurum scorzonerifolium into carrot, and provide theoretical data for the exploitation, improvement and selection of the germplasm of Chinese medicinal plants.


METHODS
The protoplasta of Bupleurum scorzonerifolium irradiated by ultraviolet light (UV) at an intensity of 300 microW.(cm2)-1 for 0, 1, 2 min respectively were fused with those of carrot Fisch by PEG method. The regenerated clones, derived form a single fused cell, were examined for their hybrid nature by phenotype and Esterase isoenzyme analysis.


RESULTS
Nine clones were identified as the somatic hybrids between B. scorzonerifolium and carrot.


CONCLUSIONS
This provides a firm foundation for the further analysis of the main active components saikosaponin of somatic hybrids and the screening out of high-medicine-content hybrid cell lines.
Huang
Xian-rong
XR
Shool of Lifescience, Shandong University, Jinan 250100, Shandong, China.
Xiang
Feng-ning
FN
Xia
Guang-min
GM
Zhi
Da-ying
DY
Shi
Jun-ying
JY
D029972:Bupleurum / Q000166:cytology / Q000235:genetics / Q000254:growth & development; D002459:Cell Fusion; D046508:Culture Techniques; D018552:Daucus carota / Q000166:cytology / Q000235:genetics / Q000254:growth & development; D004950:Esterases / Q000032:analysis; D006822:Hybrid Cells / Q000201:enzymology; D006824:Hybridization, Genetic; D007527:Isoenzymes / Q000032:analysis; D010946:Plants, Medicinal / Q000166:cytology / Q000235:genetics / Q000254:growth & development; D011523:Protoplasts / Q000166:cytology
D007527:Isoenzymes; D004950:Esterases