The cellular oxygen tension regulates expression of the endoplasmic oxidoreductase ERO1-Lalpha.
The formation of disulfide bonds in the endoplasmic reticulum requires protein disulfide isomerase (PDI) and endoplasmic reticulum oxidoreductin 1 (ERO1) that reoxidizes PDI. We report here that the expression of the rat, mouse and human homologues of ERO1-Like protein alpha but not of the isoform ERO1-Lbeta are stimulated by hypoxia in rats vivo and in rat, mouse and human cell cultures. The temporal pattern of hypoxic ERO1-Lalpha induction is very similar to that of genes triggered by the hypoxia inducible transcription factor (HIF-1) and is characteristically mimicked by cobalt and by deferoxamine, but is absent in cells with a defective aryl hydrocarbon receptor translocator (ARNT, HIF-1beta). We speculate from these findings that the expression of ERO1-Lalpha is probably regulated via the HIF-pathway and thus belongs to the family of classic oxygen regulated genes. Activation of the unfolded protein response (UPR) by tunicamycin, on the other hand, strongly induced ERO1-Lbeta and more moderately ERO1-Lalpha expression. The expression of the two ERO1-L isoforms therefore appears to be differently regulated, in the way that ERO1-Lalpha expression is mainly controlled by the cellular oxygen tension, whilst ERO1-Lbeta is triggered mainly by UPR. The physiological meaning of the oxygen regulation of ERO1-Lalpha expression likely is to maintain the transfer rate of oxidizing equivalents to PDI in situations of an altered cellular redox state induced by changes of the cellular oxygen tension.
Gess
Bernhard
B
Institut für Physiologie der Universität Regensburg, Germany.
Hofbauer
Karl-Heinz
KH
Wenger
Roland H
RH
Lohaus
Christiane
C
Meyer
Helmut E
HE
Kurtz
Armin
A
D000199:Actins / Q000378:metabolism; D053607:Adrenomedullin; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001011:Aorta / Q000166:cytology; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D004220:Disulfides; D015180:Electrophoresis, Gel, Two-Dimensional; D004721:Endoplasmic Reticulum / Q000201:enzymology*; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D000860:Hypoxia; D008562:Membrane Glycoproteins; D051379:Mice; D008954:Models, Biological; D009130:Muscle, Smooth / Q000166:cytology; D010088:Oxidoreductases; D010100:Oxygen / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D017510:Protein Folding; D020033:Protein Isoforms; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012260:Ribonucleases / Q000378:metabolism; D013997:Time Factors; D014407:Tumor Cells, Cultured; D014415:Tunicamycin / Q000494:pharmacology
D000199:Actins; D000900:Anti-Bacterial Agents; D018076:DNA, Complementary; D004220:Disulfides; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D010455:Peptides; D020033:Protein Isoforms; D012333:RNA, Messenger; D014415:Tunicamycin; D053607:Adrenomedullin; D012313:RNA; C405327:ERO1A protein, human; C491864:Ero1l protein, mouse; D010088:Oxidoreductases; D012260:Ribonucleases; D010100:Oxygen