Vaccine-induced protection against Helicobacter pylori in mice lacking both antibodies and interleukin-4.
To test the hypothesis that a Th2 response to Helicobacter pylori is necessary for protection and to address the possibility that humoral and Th2 cellular responses may compensate for each other, we generated mice deficient in both interleukin-4 (IL-4) and antibodies. The immunized double-knockout mice were protected from H. pylori challenge, as were the parental strains and wild-type C57BL/6 mice. Neutralization of IL-4 in B-cell-deficient mice did not prevent protection. Immunized IL-5-deficient mice were also protected. Thus, IL-4 and IL-5 are not essential for protection.
Garhart
Christine A
CA
Department of Pathology, Case Western Reserve University, Cleveland, Ohio 44106, USA. cag12@po.cwru.edu
Nedrud
John G
JG
Heinzel
Frederick P
FP
Sigmund
Norma E
NE
Czinn
Steven J
SJ
D000818:Animals; D000907:Antibodies, Bacterial / Q000502:physiology*; D001428:Bacterial Vaccines / Q000276:immunology*; D005756:Gastritis / Q000209:etiology; D016480:Helicobacter pylori / Q000276:immunology*; D007114:Immunization; D015847:Interleukin-4 / Q000502:physiology*; D015848:Interleukin-5 / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010641:Phenotype; D018418:Th2 Cells / Q000276:immunology*
D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D015848:Interleukin-5; D015847:Interleukin-4