title: [Relationship between mitochondrial DNA instability and interleukin-8 activity in gastric mucosa].
abstract: OBJECTIVE
  To evaluate the relationship between mitochondrial DNA instability (mtMSI) and interleukin-8 (IL-8) activity in gastric mucosa of various lesions.
  
  
  METHODS
  IL-8 level in gastric mucosa was assayed using ELISA method. The mtMSI was detected by PCR-SSCP techniques.
  
  
  RESULTS
  mtMSI was observed in 11 out of 30 (36.7%) gastric cancers, 2 of 15 (13.3%) intestinal metaplasia, 2 of 10 dysplasia and 1 of 10 chronic atrophic gastritis. IL-8 level in mtMSI+ group [(76.8 +/- 3.8) pg/mg] was significantly higher than that in mtMSI- group [(48.3 +/- 3.6) pg/mg, P < 0.05].
  
  
  CONCLUSIONS
  mtMSI closely correlates with IL-8 level in gastric mucosa and is involved in gastric carcinogenesis.
authors.affiliation: Department of Gastroenterology, Southwest Hospital, the Third Military Medical University, Chongqing 400038, China.
authors.forename: Xian-long
authors.initials: XL
authors.lastname: Ling
authors.forename: Dian-chun
authors.initials: DC
authors.lastname: Fang
authors.forename: Rong-quan
authors.initials: RQ
authors.lastname: Wang
authors.forename: Shi-ming
authors.initials: SM
authors.lastname: Yang
authors.forename: Yuan-hui
authors.initials: YH
authors.lastname: Luo
mesh_terms: D004272:DNA, Mitochondrial / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D005753:Gastric Mucosa / Q000378:metabolism* / Q000473:pathology; D005757:Gastritis, Atrophic / Q000235:genetics / Q000378:metabolism; D042822:Genomic Instability; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D008679:Metaplasia / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D011230:Precancerous Conditions / Q000235:genetics* / Q000378:metabolism; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism
chemical_list: D004272:DNA, Mitochondrial; D016209:Interleukin-8