[Dynamic observation of serological and histological changes of Chinese rhesus monkeys infected by hepatitis G virus].
OBJECTIVE
To study the genesis, development and pathogenesis of hepatitis G.


METHODS
The Chinese Rhesus monkeys were intravenously injected with the serum from a hepatitis G patient only with HGV RNA positive. The dynamic changes of serum and liver tissues of the animals were observed before and after infection. Also the immunohistochemical study were done with monocolonal antibody against NS5 gene antigen of HGV. One male monkey was dissected and the internal organs were taken for histological examination 18 months after infection.


RESULTS
Serum ALT and AST became increasing 30 days after infection and showed abnormal continuously for 9 months. Spotty and focal necrosis and piecemeal necrosis were found in liver tissue from 2 to 18 months after infection. Positive staining of HGV antigen was present in cytoplasm of liver cells by immunohistochemistry. Histological observation showed that the internal organs were normal except the liver tissue in which the damages were similar with that in hepatitis.


CONCLUSIONS
The successful infection of Chinese Rhesus monkey by serum from hepatitis G patient can used as a good animal model for the study of hepatitis G.
Xu
J
J
Institute of Liver Disease, 81 St Hospital of PLA, Nanjing 210002.
Ju
J
J
Yang
Z
Z
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D018178:Flaviviridae Infections / Q000097:blood / Q000473:pathology*; D029407:GB virus C; D006525:Hepatitis, Viral, Human / Q000097:blood / Q000473:pathology*; D008099:Liver / Q000473:pathology*; D008253:Macaca mulatta; D008297:Male