Hepatitis C virus glycoproteins mediate pH-dependent cell entry of pseudotyped retroviral particles.
HIV pseudotypes bearing native hepatitis C virus (HCV) glycoproteins (strain H and Con1) are infectious for the human hepatoma cell lines Huh-7 and PLC/PR5. Infectivity depends on coexpression of both E1 and E2 glycoproteins, is pH-dependent, and can be neutralized by mAbs mapping to amino acids 412-447 within E2. Cell-surface expression of one or all of the candidate receptor molecules (CD81, low-density lipoprotein receptor, scavenger receptor class B type 1, and dendritic cell-specific intercellular adhesion molecule 3 grabbing nonintegrin) failed to confer permissivity to HIV-HCV pseudotype infection. However, HIV-HCV pseudotype infectivity was inhibited by a recombinant soluble form of CD81 and a mAb specific for CD81, suggesting that CD81 may be a component of a receptor complex.
Hsu
Mayla
M
Aaron Diamond AIDS Research Center and Center for the Study of Hepatitis C, Laboratory of Virology and Infectious Disease, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.
Zhang
Jie
J
Flint
Mike
M
Logvinoff
Carine
C
Cheng-Mayer
Cecilia
C
Rice
Charles M
CM
McKeating
Jane A
JA
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000502:physiology; D002460:Cell Line; D002678:Chimera / Q000235:genetics / Q000276:immunology; D006678:HIV / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology; D006367:HeLa Cells; D016174:Hepacivirus / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D018937:Hepatitis C Antibodies; D022781:Hepatocytes / Q000821:virology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008565:Membrane Proteins / Q000037:antagonists & inhibitors / Q000502:physiology; D009500:Neutralization Tests; D011991:Receptors, Virus / Q000037:antagonists & inhibitors / Q000502:physiology; D060225:Tetraspanin 28; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000502:physiology*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; C554798:CD81 protein, human; C087035:E1 protein, Hepatitis C virus; D018937:Hepatitis C Antibodies; D008565:Membrane Proteins; D011991:Receptors, Virus; D060225:Tetraspanin 28; D014759:Viral Envelope Proteins; C091015:glycoprotein E2, Hepatitis C virus