title: Skin replacement with a collagen based dermal substitute, autologous keratinocytes and fibroblasts in burn trauma.
abstract: This case report describes the successful wound management of a 19-year-old patient with 76% of his body surface burnt. Due to the severity of the burns and the limited availability of native skin, 63% of the body surface were covered with Integra, a dermis equivalent. This material has been developed for the reconstruction of dermal structures and is designed as a bilayer. It consists of a modified, bovine collagen matrix and a silastic membrane facing toward the body surface. After vascularization of Integra, the epidermal skin replacement was created in a second step. In four different therapy procedures autologous, cultivated keratinocytes were combined with autologous fibroblast suspensions of various concentration and split-thickness skin as a 1:6 mesh graft. Rapid and largely complication-free wound healing could be achieved despite the limited availability of native skin. A correlation could be observed between the time of complete wound closure and the number of fibroblasts applied. The patient was monitored over a period of 1 year and showed good functional and cosmetic results.
authors.affiliation: Department of Hand, Plastic, Reconstructive and Burn Surgery, Schnarrenbergstr 95, BG-Unfallklinik, 72076 Tuebingen, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Wisser
authors.forename: J
authors.initials: J
authors.lastname: Steffes
mesh_terms: D000328:Adult; D001830:Body Surface Area; D002056:Burns / Q000601:surgery / Q000628:therapy*; D003094:Collagen / Q000008:administration & dosage; D005347:Fibroblasts / Q000637:transplantation*; D006801:Humans; D015603:Keratinocytes / Q000637:transplantation*; D008297:Male; D017060:Patient Satisfaction; D017768:Skin, Artificial; D016896:Treatment Outcome; D014945:Wound Healing
chemical_list: D003094:Collagen