title: Intracytoplasmic domains of MHC class II molecules are essential for lipid-raft-dependent signaling.
abstract: In addition to their role in antigen presentation, major histocompatibility complex (MHC) class II molecules have been widely described as signaling proteins in diverse antigen-presenting cells (APCs) including B cells and dendritic cells. By contrast, little is known of the signaling function of MHC class II molecules expressed in solid tumors. We describe the functional organization and signaling ability of I-Ak expressed in a sarcoma, and report the recruitment of I-Ak to lipid rafts after MHC class II engagement. Lipid raft integrity was required for I-Ak-mediated reorganization of the actin cytoskeleton and translocation of protein kinase C-alpha(PKC-alpha) to the precise site of stimulation via I-Ak. Truncation of the intracytoplasmic domains of I-Ak did not perturb I-Ak recruitment to lipid rafts but abrogated PKC-alpha translocation and actin rearrangement. PKC-alpha was detected in lipid microdomains and enrichment of activated PKC-alphain lipid rafts was induced by I-Ak signaling. Ordering of the molecular events following engagement of the MHC class II molecules revealed that I-Ak recruitment to lipid rafts precedes signaling. This is consistent with the absence of a requirement for the intracytoplasmic tails for localization to lipid rafts. These data reveal that lipid-rich microdomains play a key role in MHC class II-mediated signaling in a solid tumor.
authors.affiliation: Unité INSERM U 396, Institut Biomédical des Cordeliers, 15 rue de l'Ecole de Médecine, 75006 Paris, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Bécart
authors.forename: Niclas
authors.initials: N
authors.lastname: Setterblad
authors.forename: Suzanne
authors.initials: S
authors.lastname: Ostrand-Rosenberg
authors.forename: Santa J
authors.initials: SJ
authors.lastname: Ono
authors.forename: Dominique
authors.initials: D
authors.lastname: Charron
authors.forename: Nuala
authors.initials: N
authors.lastname: Mooney
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D000818:Animals; D045744:Cell Line, Tumor; D003593:Cytoplasm / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000378:metabolism*; D021962:Membrane Microdomains / Q000378:metabolism*; D051379:Mice; D011493:Protein Kinase C / Q000378:metabolism; D051571:Protein Kinase C-alpha; D017434:Protein Structure, Tertiary / Q000502:physiology; D021381:Protein Transport / Q000502:physiology; D012509:Sarcoma / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology
chemical_list: D000949:Histocompatibility Antigens Class II; C111388:I-Ak antigen; C496630:Prkca protein, mouse; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha