title: [High-dose glucose induces human retinal endothelial cell apoptosis].
abstract: OBJECTIVE
  To examine the direct effect of high glucose levels on primary cultured human retinal capillary endothelial cells (HRCEC).
  
  
  METHODS
  HRCECs were isolated from donated eyes and cultured for 6 days in the media containing 5 or 25 mmol/L glucose. The cell viability was determined by trypan blue exclusion assay and cell cycle analyzed by flow cytometry, with the cell apoptosis assayed by TUNEL method.
  
  
  RESULTS
  The cell viability was significantly decreased after exposure to 25 mmol/L glucose, and the number of apoptotic cells determined by flow cytometry and TUNEL was significantly increased in response to high-dose glucose treatment.
  
  
  CONCLUSIONS
  High-dose glucose induces apoptosis in HRCEC, which may contribute to the development of diabetic retinopathy.
authors.affiliation: Department of Internal Medicine, 476 Army Hospital, Fuzhou, 350002, China.
authors.forename: Jian-fang
authors.initials: JF
authors.lastname: Ma
authors.forename: Zhong-han
authors.initials: ZH
authors.lastname: Yang
authors.forename: Zhi-hong
authors.initials: ZH
authors.lastname: Song
authors.forename: Yin
authors.initials: Y
authors.lastname: Guo
authors.forename: Wei-bin
authors.initials: WB
authors.lastname: Cai
authors.forename: Qian-ping
authors.initials: QP
authors.lastname: Liu
authors.forename: Lin-lang
authors.initials: LL
authors.lastname: Guo
authors.forename: Guo-quan
authors.initials: GQ
authors.lastname: Gao
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003930:Diabetic Retinopathy / Q000209:etiology*; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000473:pathology; D005947:Glucose / Q000633:toxicity*; D006801:Humans; D012160:Retina / Q000187:drug effects* / Q000473:pathology
chemical_list: D005947:Glucose