Molecular cloning and characterization of a novel human J-domain protein gene (HDJ3) from the fetal brain.
The J-domain is believed to be part of a chaperone involved in protein folding. From a fetal brain cDNA library, we isolated a cDNA of 3249 bp encoding a novel human J-domain protein, which was named as HDJ3. The expression pattern of HDJ3 was examined by reverse transcription/polymerase chain reaction, which suggested that the transcripts were highly expressed in human pancreas and selectively expressed in human brain, lung, liver, skeletal muscle and kidney. The results also showed that a probable splice variant of HDJ3 gene might exist. The HDJ3 gene was located on human chromosome 12q13.1-12q13.2 and consisted of seven exons spanning 8593 bp of the human genome. PSORT analysis indicated that the HDJ3 gene contained a transmembrane domain. The putative protein of the HDJ3 gene was highly homologous to rat dopamine-receptor-interacting protein, suggesting that it was a novel member of the molecular chaperone family and functionally related to dopamine signal transduction.
Chen
Juxiang
J
Department of Neurosurgery, Chang Zheng Hospital, Second Military Medical University, 200003, Shanghai, People's Republic of China.
Huang
Yan
Y
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, 200433, Shanghai , P.R. China.|United Gene Holdings, 200092, Shanghai, P.R. China.
Wu
Hai
H
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, 200433, Shanghai , P.R. China.
Ni
Xiaohua
X
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, 200433, Shanghai , P.R. China.
Cheng
Haipeng
H
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, 200433, Shanghai , P.R. China.
Fan
Jingping
J
Department of Neurosurgery, Chang Zheng Hospital, Second Military Medical University, 200003, Shanghai, People's Republic of China.
Gu
Shaohua
S
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, 200433, Shanghai , P.R. China.
Gu
Xing
X
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, 200433, Shanghai , P.R. China.
Cao
Gentao
G
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, 200433, Shanghai , P.R. China.
Ying
Kang
K
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, 200433, Shanghai , P.R. China.
Mao
Yumin
Y
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, 200433, Shanghai , P.R. China.
Lu
Yicheng
Y
Department of Neurosurgery, Chang Zheng Hospital, Second Military Medical University, 200003, Shanghai, People's Republic of China.
Xie
Yi
Y
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, 200433, Shanghai , P.R. China. yxie@fudan.edu.cn.
D000595:Amino Acid Sequence; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D002881:Chromosomes, Human, Pair 12; D003001:Cloning, Molecular; D005326:Fetal Proteins / Q000235:genetics*; D005333:Fetus; D015870:Gene Expression; D006801:Humans; D018832:Molecular Chaperones / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009928:Organ Specificity; D010179:Pancreas / Q000378:metabolism; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid
C476535:DNAJC14 protein, human; D005326:Fetal Proteins; D018832:Molecular Chaperones