title: Preliminary evaluation of several disinfection/sterilization techniques for use with microdialysis probes.
abstract: This study evaluated the suitability of some disinfection and sterilization methods for use with microdialysis probes. Disinfection or sterilization should minimize the tissue inflammatory reaction and improve the long-term health of rats on study and ensure the quality of data obtained by microdialysis sampling. Furthermore, the treatment should not negatively impact probe integrity or sampling performance. The techniques chosen for evaluation included two disinfection methods (70% ethanol and a commercial contact lens solution) and two sterilization methods (hydrogen peroxide plasma, and e-beam radiation). Linear microdialysis probes treated by these processes were compared to untreated probes removed from the manufacturer's packaging as if sterile (the control group). The probes were aseptically implanted in the livers of rats and monitored for 72 hours. The parameters chosen to evaluate probe performance were relative sample mass recovery and the relative in vivo extraction efficiency of the probe for caffeine. Post mortem bacterial counts and histopathology examination of liver tissue were also conducted. The probes remained intact and functional for the entire study period. The methods tested did not acutely alter the probes although hydrogen peroxide plasma and contact lens solution groups showed reduced extraction efficiencies. Minimal tissue damage was observed surrounding the probes and acute inflammatory reaction was mild to moderate. Low numbers of bacterial colonies from the implantation sites indicates that the health of animals in this study was not impaired. This was also true for the control group (untreated probe).
authors.affiliation: Department of Molecular Biosciences, The University of Kansas, Lawrence, KS, USA.
authors.forename: Jacquelyn K
authors.initials: JK
authors.lastname: Huff
authors.forename: James F
authors.initials: JF
authors.lastname: Bresnahan
authors.forename: Malonne I
authors.initials: MI
authors.lastname: Davies
mesh_terms: D000818:Animals; D001610:Beta Particles; D002110:Caffeine / Q000032:analysis; D015169:Colony Count, Microbial; D018639:Contact Lens Solutions / Q000494:pharmacology; D004202:Disinfectants / Q000494:pharmacology*; D004203:Disinfection / Q000379:methods*; D004866:Equipment Contamination / Q000517:prevention & control*; D000431:Ethanol / Q000494:pharmacology; D005110:Extracellular Space / Q000382:microbiology; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008099:Liver / Q000382:microbiology / Q000473:pathology; D008297:Male; D017551:Microdialysis / Q000295:instrumentation / Q000592:standards*; D019736:Prostheses and Implants; D051381:Rats; D017207:Rats, Sprague-Dawley; D013242:Sterilization / Q000379:methods*
chemical_list: D018639:Contact Lens Solutions; D004202:Disinfectants; D002110:Caffeine; D000431:Ethanol; D006861:Hydrogen Peroxide