Caenorhabditis elegans early embryogenesis and vulval morphogenesis require chondroitin biosynthesis.
Defects in glycosaminoglycan biosynthesis disrupt animal development and can cause human disease. So far much of the focus on glycosaminoglycans has been on heparan sulphate. Mutations in eight squashed vulva (sqv) genes in Caenorhabditis elegans cause defects in cytokinesis during embryogenesis and in vulval morphogenesis during postembryonic development. Seven of the eight sqv genes have been shown to control the biosynthesis of the glycosaminoglycans chondroitin and heparan sulphate. Here we present the molecular identification and characterization of the eighth gene, sqv-5. This gene encodes a bifunctional glycosyltransferase that is probably localized to the Golgi apparatus and is responsible for the biosynthesis of chondroitin but not heparan sulphate. Our findings show that chondroitin is crucial for both cytokinesis and morphogenesis during C. elegans development.
Hwang
Ho-Yon
HY
Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Room 68-425, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
Olson
Sara K
SK
Esko
Jeffrey D
JD
Horvitz
H Robert
HR
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D017173:Caenorhabditis elegans / Q000196:embryology* / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002807:Chondroitin / Q000096:biosynthesis*; D003001:Cloning, Molecular; D005260:Female; D017353:Gene Deletion; D020869:Gene Expression Profiling; D016695:Glycosyltransferases / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006497:Heparan Sulfate / Q000096:biosynthesis; D008969:Molecular Sequence Data; D009024:Morphogenesis; D016415:Sequence Alignment; D013379:Substrate Specificity; D014844:Vulva / Q000196:embryology* / Q000201:enzymology / Q000378:metabolism*
D029742:Caenorhabditis elegans Proteins; D002807:Chondroitin; D016695:Glycosyltransferases; D006497:Heparan Sulfate; C474582:sqv-5 protein, C elegans