Th2 immune responses in GATA-3-transgenic mice infected with Heligmosomoides polygyrus.
To examine the participation of transcription factor GATA-3 in Th2 immune responses in vivo, we generated transgenic mice having several copies of GATA-3 with LCK promotor. Mice were infected with the intestinal nematode Heligmosomoides polygyrus to induce Th2 immune responses. Upon antigen stimulation, IL-5 and IL-13 production of mesenteric lymph node cells from H. polygyrus-infected mice, was significantly enhanced in GATA-3-transgenic mice compared with nontransgenic control mice. However, IL-4 production was the same in GATA-3-transgenic and control mice. H. polygyrus-infected GATA-3-transgenic mice exhibited significantly more peripheral blood eosinophils and total IgE levels compared with control mice. These results suggest that GATA-3 promotes IL-5 and IL-13 production, and that the function of these cytokines results in eosinophilia and hyper-IgE, respectively.
Watanabe
Naohiro
N
Department of Tropical Medicine, Jikei University School of Medicine, Tokyo, Japan. naohiro@jikei.ac.jp
Tamauchi
Hidekazu
H
Ozawa
Hideyuki
H
Ito
Mamoru
M
Ovary
Zoltan
Z
Habu
Sonoko
S
D000818:Animals; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000276:immunology* / Q000378:metabolism*; D004195:Disease Models, Animal; D004802:Eosinophilia / Q000276:immunology / Q000378:metabolism; D004804:Eosinophils / Q000378:metabolism; D050990:GATA3 Transcription Factor; D020397:Goblet Cells / Q000378:metabolism; D007073:Immunoglobulin E / Q000378:metabolism; D007421:Intestine, Small / Q000166:cytology / Q000378:metabolism; D007958:Leukocyte Count; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic / Q000469:parasitology*; D009351:Nematospiroides dubius; D010270:Parasite Egg Count; D017206:Strongylida Infections / Q000276:immunology* / Q000378:metabolism*; D018418:Th2 Cells / Q000276:immunology* / Q000378:metabolism*; D015534:Trans-Activators / Q000276:immunology* / Q000378:metabolism*
D016207:Cytokines; D004268:DNA-Binding Proteins; D050990:GATA3 Transcription Factor; C494748:Gata3 protein, mouse; D015534:Trans-Activators; D007073:Immunoglobulin E