title: giant nuclei is essential in the cell cycle transition from meiosis to mitosis.
abstract: At the transition from meiosis to cleavage mitoses, Drosophila requires the cell cycle regulators encoded by the genes, giant nuclei (gnu), plutonium (plu) and pan gu (png). Embryos lacking Gnu protein undergo DNA replication and centrosome proliferation without chromosome condensation or mitotic segregation. We have identified the gnu gene encoding a novel phosphoprotein dephosphorylated by Protein phosphatase 1 at egg activation. Gnu is normally expressed in the nurse cells and oocyte of the ovary and is degraded during the embryonic cleavage mitoses. Ovarian death and sterility result from gnu gain of function. gnu function requires the activity of pan gu and plu.
authors.affiliation: Department of Zoology, University of Oxford, South Parks Road, Oxford, OX1 3PS, UK.
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Renault
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Zhang
authors.forename: Luke S
authors.initials: LS
authors.lastname: Alphey
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Frenz
authors.forename: David M
authors.initials: DM
authors.lastname: Glover
authors.forename: Robert D C
authors.initials: RD
authors.lastname: Saunders
authors.forename: J Myles
authors.initials: JM
authors.lastname: Axton
mesh_terms: D000199:Actins / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D029721:Drosophila Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology / Q000196:embryology* / Q000502:physiology; D004843:Epistasis, Genetic; D005260:Female; D018507:Gene Expression Regulation, Developmental; D007246:Infertility; D008297:Male; D008540:Meiosis / Q000502:physiology*; D008938:Mitosis / Q000502:physiology*; D008969:Molecular Sequence Data; D009865:Oocytes / Q000166:cytology / Q000502:physiology; D010053:Ovary / Q000166:cytology / Q000502:physiology; D010641:Phenotype; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D019076:Transgenes
chemical_list: D000199:Actins; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C085874:PLU protein, Drosophila; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; C477039:gnu protein, Drosophila; C417221:png protein, Drosophila; D017346:Protein Serine-Threonine Kinases