Glutamate induced currents in isolated inner hair cells from guinea-pig cochlea.
We investigated the direct action of glutamate (Glu) on the membrane current of isolated inner hair cells of guinea-pig cochlea. Glu elicited inward currents at a holding potential of -70 mV. Eight of 13 cells showed a steady inward current, while five of 13 cells showed a fast and rapidly desensitized current. I-V relationships demonstrated that the reversal potential of Glu-induced current was near 0 mV. Glu-induced currents were dose-dependent, where the half maximum concentration (K(d)) was 41 microM and Hill coefficient (n) was 1.75.
Kimitsuki
Takashi
T
Department of Otorhinolaryngology, Miyazaki Medical College, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan. kimituki@fc.miyazaki-med.ac.jp
Ohashi
Mitsuru
M
Wada
Yuki
Y
Okuda
Takumi
T
Komune
Shizuo
S
D000818:Animals; D003051:Cochlea / Q000166:cytology / Q000502:physiology*; D018698:Glutamic Acid / Q000494:pharmacology* / Q000502:physiology; D006168:Guinea Pigs; D006199:Hair Cells, Auditory, Inner / Q000502:physiology*; D066298:In Vitro Techniques; D008564:Membrane Potentials; D018408:Patch-Clamp Techniques
D018698:Glutamic Acid