Alterations of HDL subclasses in hyperlipidemia.
BACKGROUND
It is generally accepted that different high-density lipoprotein (HDL) subclasses have distinct but interrelated metabolic functions. HDL is known to directly influence the atherogenic process and changes in HDL subclasses distribution may be related to the incidence and prevalence of atherosclerosis.


METHODS
The relative apolipoprotein (apo)A-I contents (% apoA-I) of plasma HDL subclasses were determined by two-dimensional gel electrophoresis coupled with immunodetection for apoA-I, in 39 hypercholesterolemic (HTC) subjects, 97 hypertriglyceridemic (HTG) subjects and 32 mixed hyperlipidemic (MHL) subjects, and 124 normolipidemic subjects.


RESULTS
The relative apoA-I contents of prebeta(1)-HDL, prebeta(2)-HDL, HDL(3c), HDL(3b) and HDL(3a) significantly increased while HDL(2a) and HDL(2b) significantly decreased in hyperlipidemic subjects. In HTC subjects of hyperlipidemia, the concentrations of prebeta(1)-HDL were significantly lower and HDL(2b) concentrations were significantly higher than in HTG and MHL subjects. In HTG subjects, the concentrations of HDL(3a) were significantly higher and the concentrations of HDL(2b) were lower than in HTC and MHL subjects. In total hyperlipidemic subjects, plasma triglyceride (TG) concentrations showed positive correlation with prebeta(1)-HDL, prebeta(2)-HDL, HDL(3b) and HDL(3a) and negative correlation with HDL(2a) and HDL(2b). The total cholesterol (TC) concentrations showed positive correlation with the relative apoA-I contents of prebeta(1)-HDL and HDL(3b), whereas the HDL-C concentrations showed negative correlation with the relative apoA-I contents of prebeta(1)-HDL and HDL(3a) and positive correlation with those of HDL(2a) and HDL(2b). The relative apoA-I contents of prebeta(1)-HDL, prebeta(2)-HDL, HDL(3b), and HDL(3a) were positively correlated whereas those of HDL(2a) and HDL(2b) were negatively correlated with TG/HDL-C ratio.


CONCLUSIONS
The particle size of HDL in hyperlipidemic subjects shifted towards smaller sizes, which, in turn, indicates that the maturation of HDL may be abnormal in hyperlipidemic subjects.
Xu
Yanhua
Y
Department of Biochemistry and Apolipoprotein Research Unit, West China Medical Center, Sichuan University, Chengdu, 610041 Sichuan, People's Republic of China.
Fu
Mingde
M
D000328:Adult; D000368:Aged; D016632:Apolipoprotein A-I / Q000032:analysis / Q000097:blood*; D001161:Arteriosclerosis / Q000209:etiology*; D005215:Fasting / Q000097:blood; D005260:Female; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood; D006949:Hyperlipidemias / Q000097:blood* / Q000150:complications; D006954:Hyperlipoproteinemia Type V / Q000097:blood; D015228:Hypertriglyceridemia / Q000097:blood; D008075:Lipoproteins, HDL / Q000097:blood / Q000737:chemistry / Q000145:classification*; D008297:Male; D008875:Middle Aged; D010316:Particle Size; D013223:Statistics as Topic
D016632:Apolipoprotein A-I; D008075:Lipoproteins, HDL