title: Sialic acid receptor specificity on erythrocytes affects detection of antibody to avian influenza haemagglutinin.
abstract: Haemagglutination-inhibition tests (HI) are used to detect increases in influenza antibody in serum. However, they are relatively insensitive for the detection of human antibody responses to avian haemagglutinin, even in the presence of high titres of neutralising antibody after confirmed infection or vaccination. Human influenza viruses bind preferentially sialic acid containing N-acetylneuraminic acid alpha2,6-galactose (SAalpha2,6Gal) linkages while avian and equine viruses bind preferentially those containing N-acetylneuraminic acid alpha2,3-galactose (SAalpha2,3Gal) linkages. Increasing the proportion of SAalpha2,3Gal linkages on the erythrocytes used, by enzymatic modification or change of species, improves the ability of erythrocytes to bind to avian influenza strains and thereby improves the sensitivity of detection of antibody to avian and equine HA in a range of mammalian and human sera using HI tests.
authors.affiliation: Enteric, Respiratory and Neurological Laboratory, Public Health Laboratory Service, London, United Kingdom.
authors.forename: I
authors.initials: I
authors.lastname: Stephenson
authors.forename: J M
authors.initials: JM
authors.lastname: Wood
authors.forename: K G
authors.initials: KG
authors.lastname: Nicholson
authors.forename: M C
authors.initials: MC
authors.lastname: Zambon
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D004912:Erythrocytes / Q000737:chemistry / Q000378:metabolism*; D005289:Ferrets; D006041:Goats; D006385:Hemagglutination Inhibition Tests; D006387:Hemagglutination, Viral / Q000276:immunology; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000378:metabolism; D006736:Horses; D006801:Humans; D007106:Immune Sera / Q000032:analysis*; D009980:Influenza A virus / Q000276:immunology* / Q000378:metabolism; D037102:Lectins / Q000378:metabolism*; D011817:Rabbits; D011991:Receptors, Virus / Q000378:metabolism*; D012756:Sheep; D063265:Sialic Acid Binding Immunoglobulin-like Lectins; D013045:Species Specificity; D014422:Turkeys
chemical_list: D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007106:Immune Sera; D037102:Lectins; D011991:Receptors, Virus; D063265:Sialic Acid Binding Immunoglobulin-like Lectins