title: Inhibition of nitric oxide production on LPS-activated macrophages by kazinol B from Broussonetia kazinoki.
abstract: The ethyl acetate soluble fraction of Broussonetia kazinoki at 50 microg/ml showed a significant inhibition (78.2%) of nitric oxide (NO) in lipopolysaccharide activated macrophages. Kazinol B, an isoprenylated flavan, was identified as the active principle. It inhibits the NO synthesis with an IC(50) of 21.6 microM.
authors.affiliation: College of Pharmacy, Sookmyung Women's University, Seoul 140-742, South Korea. ryuha@sdic.sookmyung.ac.kr
authors.forename: Jae-Ha
authors.initials: JH
authors.lastname: Ryu
authors.forename: Hanna
authors.initials: H
authors.lastname: Ahn
authors.forename: Hwa
authors.initials: H
authors.lastname: Jin Lee
mesh_terms: D000818:Animals; D015153:Blotting, Western; D031623:Broussonetia; D004591:Electrophoresis, Polyacrylamide Gel; D005419:Flavonoids / Q000008:administration & dosage / Q000494:pharmacology*; D005638:Fruit; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D019001:Nitric Oxide Synthase / Q000096:biosynthesis*; D052247:Nitric Oxide Synthase Type II; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology; D018515:Plant Leaves
chemical_list: D005419:Flavonoids; D008070:Lipopolysaccharides; D010936:Plant Extracts; C117415:kazinol B; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse