[Relationship between mitochondrial DNA instability and interleukin-8 activity in gastric mucosa].
OBJECTIVE
To evaluate the relationship between mitochondrial DNA instability (mtMSI) and interleukin-8 (IL-8) activity in gastric mucosa of various lesions.


METHODS
IL-8 level in gastric mucosa was assayed using ELISA method. The mtMSI was detected by PCR-SSCP techniques.


RESULTS
mtMSI was observed in 11 out of 30 (36.7%) gastric cancers, 2 of 15 (13.3%) intestinal metaplasia, 2 of 10 dysplasia and 1 of 10 chronic atrophic gastritis. IL-8 level in mtMSI+ group [(76.8 +/- 3.8) pg/mg] was significantly higher than that in mtMSI- group [(48.3 +/- 3.6) pg/mg, P < 0.05].


CONCLUSIONS
mtMSI closely correlates with IL-8 level in gastric mucosa and is involved in gastric carcinogenesis.
Ling
Xian-long
XL
Department of Gastroenterology, Southwest Hospital, the Third Military Medical University, Chongqing 400038, China.
Fang
Dian-chun
DC
Wang
Rong-quan
RQ
Yang
Shi-ming
SM
Luo
Yuan-hui
YH
D004272:DNA, Mitochondrial / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D005753:Gastric Mucosa / Q000378:metabolism* / Q000473:pathology; D005757:Gastritis, Atrophic / Q000235:genetics / Q000378:metabolism; D042822:Genomic Instability; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D008679:Metaplasia / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D011230:Precancerous Conditions / Q000235:genetics* / Q000378:metabolism; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism
D004272:DNA, Mitochondrial; D016209:Interleukin-8