title: Analysis of urinary nucleosides. III. Identification of 5'-deoxycytidine in urine of a patient with head and neck cancer.
abstract: Modified nucleosides in the urine have been postulated to be diagnostic indices of disease, particularly of cancer. The urine of a patient with terminal head and neck cancer has been found to contain a modified nucleoside with a protonated molecule of m/z 228. By means of high-performance liquid chromatography/ion trap mass spectrometry (HPLC/ITMS) and capillary liquid chromatography/triple quadruple mass spectrometry (CapLC/TQMS) we have identified the compound as 5'-deoxycytidine. This is the first report of 5'-deoxycytidine in man: in addition to the elucidation of its structure, its possible origins and the potential significance of its occurrence are discussed.
authors.affiliation: Biochemistry Group, School of Biological Sciences, Wallace Building, University of Wales Swansea, Singleton Park, Swansea SA2 8PP, UK.
authors.forename: Edward
authors.initials: E
authors.lastname: Dudley
authors.forename: Filip
authors.initials: F
authors.lastname: Lemiere
authors.forename: Walter
authors.initials: W
authors.lastname: Van Dongen
authors.forename: James I
authors.initials: JI
authors.lastname: Langridge
authors.forename: Salah
authors.initials: S
authors.lastname: El-Sharkawi
authors.forename: David E
authors.initials: DE
authors.lastname: Games
authors.forename: Eddy L
authors.initials: EL
authors.lastname: Esmans
authors.forename: Russell P
authors.initials: RP
authors.lastname: Newton
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003841:Deoxycytidine / Q000652:urine*; D006258:Head and Neck Neoplasms / Q000652:urine*; D006801:Humans; D013058:Mass Spectrometry
chemical_list: D003841:Deoxycytidine