Transposition in Lactobacillus delbrueckii subsp. bulgaricus: identification of two thermosensitive replicons and two functional insertion sequences.
In this report, it is shown that the rolling circle replicon pG(+)host and the theta replicon pIP501 are thermosensitive in Lactobacillus delbrueckii subsp. bulgaricus (Lactobacillus bulgaricus). Using a pIP501 derivative as a delivery vector for six insertion sequences originating from lactic acid bacteria, it is shown that IS1223 and IS1201 transpose in L. bulgaricus.
Serror
Pascale
P
Unité Recherche Laitière et Génétique Appliquée, INRA, Domaine de Vilvert, 78352 Jouy-en-Josas Cedex, France.
Ilami
Golnar
G
Génétique Microbienne, INRA, Domaine de Vilvert, 78352 Jouy-en-Josas Cedex, France.
Chouayekh
Hichem
H
Génétique Microbienne, INRA, Domaine de Vilvert, 78352 Jouy-en-Josas Cedex, France.
Ehrlich
S Dusko
SD
Génétique Microbienne, INRA, Domaine de Vilvert, 78352 Jouy-en-Josas Cedex, France.
Maguin
Emmanuelle
E
Génétique Microbienne, INRA, Domaine de Vilvert, 78352 Jouy-en-Josas Cedex, France.
D001483:Base Sequence; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D007778:Lactobacillus / Q000235:genetics*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000379:methods; D010957:Plasmids / Q000235:genetics; D012093:Replicon; D013696:Temperature
D004251:DNA Transposable Elements; D004269:DNA, Bacterial