[Determination of salvianolic acid B in the radix of Salvia miltiorrhiza Bge. by HPLC].
OBJECTIVE
To develop an HPLC method for determining salvianolic acid B in radix of Salvia miltiorrhiza.


METHODS
The sample was extracted with 75% methanol. ODS column was used with methanol-5% acetic acid solution (35:65) as mobile phase. Detection wavelength was 281 nm.


RESULTS
Salvianolic acid B was separated well. Good linearity of salvianolic acid B was obtained (r = 0.9997) within the range of 0.17-1.7 micrograms. The average recovery was 98.9%. Repeatability was good, and RSD was 1.82%.


CONCLUSIONS
This method can be used for quality control of radix of Salvia miltiorrhiza.
Zhang
Q W
QW
Institute of Chinese Materia Medica, China Academy of TCM, Beijing 100700, China.
Zhang
Y
Y
Li
J P
JP
Ma
H
H
D001572:Benzofurans / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D053630:Depsides
D001572:Benzofurans; C076944:salvianolic acid B; D053630:Depsides