Photophysical properties of 5-methylcytidine.
Cytosine methylation, which determines the hot spots for DNA photo-damage, is shown to induce a red-shift of the nucleoside absorption spectrum, making the chromophore more vulnerable to solar radiation, and a tenfold increase of the fluorescence lifetime, making excited statereactions more probable. A femtosecond investigation of the excited state deactivation reveals a quite complex mechanism.
Sharonov
Alexei
A
Laboratoire Francis Perrin, CEA/DSM/DRECAM/SPAM - CNRS URA 2453, CEA Saclay, 91191 Gif-sur-Yvette, France.
Gustavsson
Thomas
T
Marguet
Sylvie
S
Markovitsi
Dimitra
D
D003562:Cytidine / Q000031:analogs & derivatives* / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D003562:Cytidine; C016568:5-methylcytidine