[Expression of estradiol receptors in mast cells of the upper airway].
OBJECTIVE
To examine the expression of estrogen receptors in mast cells, CD4+, CD8+ and CD68+ cells in human nasal polyps.


METHODS
Immunohistochemical and dual immunostaining methods were used with mouse monoclonal antibodies against human estrogen receptors, a range of human immune cell types included CD4+ (helper T-cells), CD8+ (suppressor/cytotoxic T-cells), CD68+ (Macrophages) cells and mast cells.


RESULTS
Coincident with the known features of human nasal polyps, all the immune/inflammatory cell types examined were clearly detected with corresponding monoclonal antibodies. The cells positive for estrogen receptor were morphologically similar to the mast cells but distinct from CD4+, CD8+ or CD68+ cells. The cells stained for estrogen receptors by dual immunostaining coincided exactly with cells labeled as mast cells but differed from CD4+, CD8+ or CD68+ cells.


CONCLUSIONS
Estrogen receptors express only in mast cells, but not in CD4+, CD8+ or CD68+ cells in the human upper airway.
Zhao
X
X
Department of ENT & Head-Neck Surgery, Third Teaching Hospital, Bethune University of Medical Sciences, Changchun 130031.
Dong
Z
Z
Yang
Z
Z
D000328:Adult; D000368:Aged; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008407:Mast Cells / Q000378:metabolism*; D008875:Middle Aged; D009298:Nasal Polyps / Q000378:metabolism* / Q000473:pathology; D011959:Receptors, Estradiol / Q000378:metabolism*
D011959:Receptors, Estradiol