title: Vitamin E supplementation in the mitigation of carbon tetrachloride induced oxidative stress in rats.
abstract: Oxidative stress is implicated in the pathophysiology of a number of chronic diseases including atherosclerosis, diabetes, cataracts and accelerated aging. The aim of this study was to elucidate the protective role of vitamin E supplementation when oxidative stress is induced by CCl4 administration, using the rat as a model. Rats were fed diets for four weeks either with or without dl-alpha-tocopherol acetate supplementation. Half of the rats (n = 9) from each of the diet groups were then challenged with CCl4 at the completion of the four week diet period. Plasma levels of 8-iso-PGF(2alpha), antioxidant micronutrients and antioxidant enzyme activities were measured to examine changes in oxidative stress subsequent to the supplementation of dl-alpha-tocopherol in the diet. Plasma alpha-tocopherol (vitamin E) concentrations were higher for the groups supplemented with dl-alpha-tocopherol acetate, however the supplemented diet group that was subsequently challenged with CCl4 had significantly lower (p <0.001) plasma alpha-tocopherol concentration than the dl-alpha-tocopherol acetate diet group that was not challenged with CCl4. Total plasma 8-iso-PGF(2alpha) concentration was elevated in diet groups challenged with CCl4, however, the concentration was significantly lower (p <0.001) when the diet was supplemented with dl-alpha-tocopherol acetate. The antioxidant enzymes were not influenced by either dietary alpha-tocopherol manipulation or by the inducement of oxidative stress with CCl4. Plasma concentrations of trans-retinol (vitamin A) were reduced by CCl4 administration in both the dl-alpha-tocopherol acetate supplemented and unsupplemented diet groups. The results of this study indicate that dl-alpha-tocopherol acetate supplementation was protective of lipid peroxidation when oxidative stress is induced by a pro-oxidant challenge such as CCl4.
authors.affiliation: Nutrition and Dietetics, School of Health Science, Faculty of Health, University of Newcastle, 2308 Callaghan, NSW, Australia.
authors.forename: Lesley K
authors.initials: LK
authors.lastname: MacDonald-Wicks
authors.forename: Manohar L
authors.initials: ML
authors.lastname: Garg
mesh_terms: D000818:Animals; D002251:Carbon Tetrachloride; D004032:Diet; D019587:Dietary Supplements; D015237:Dinoprost / Q000031:analogs & derivatives*; D004910:Erythrocyte Membrane / Q000737:chemistry; D028441:F2-Isoprostanes / Q000097:blood; D005227:Fatty Acids / Q000097:blood; D005260:Female; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D024505:Tocopherols; D014801:Vitamin A / Q000097:blood; D024502:alpha-Tocopherol / Q000008:administration & dosage* / Q000031:analogs & derivatives* / Q000097:blood
chemical_list: D028441:F2-Isoprostanes; D005227:Fatty Acids; D014801:Vitamin A; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost; D002251:Carbon Tetrachloride; D024502:alpha-Tocopherol; D024505:Tocopherols