title: Cytotoxicity of a new endodontic filling material.
abstract: In vitro cell cultures have been widely used as a means of evaluating cytotoxicity of root canal filling materials. Following ANSI/ADA spec. no. 41, the aim of the present study was to investigate the biological compatibility of a new sealer (FibreFill) and compare it with some commercially available endodontic sealers (Bioseal and Acroseal). Mouse 3T3 fibroblasts were seeded and cultured and subsequently extracts of the cements were added. After 24 hours incubation, the cellular vitality of fibroblasts was evaluated by the neutral red uptake test (NRU), which measures the membrane permeability. Data were collected and statistically analysed. Results showed that all tested materials exhibited mild cytotoxic effects, which are compatible with normal clinical use, and no statistically significant difference was noted between FibreFill and the other tested materials. Therefore, selection amongst these sealers should be based on other factors.
authors.affiliation: University of Rome La Sapienza, Dental School, Italy. ggambarini@tin.it
authors.forename: G
authors.initials: G
authors.lastname: Gambarini
authors.forename: M
authors.initials: M
authors.lastname: Andreasi-Bassi
authors.forename: G
authors.initials: G
authors.lastname: Bolognini
authors.forename: L
authors.initials: L
authors.lastname: Testarelli
authors.forename: G
authors.initials: G
authors.lastname: Nocca
authors.forename: L
authors.initials: L
authors.lastname: Ceccarelli
authors.forename: R
authors.initials: R
authors.lastname: Scatena
authors.forename: A
authors.initials: A
authors.lastname: Lupi
authors.forename: M
authors.initials: M
authors.lastname: Castagnola
mesh_terms: D016475:3T3 Cells / Q000187:drug effects; D000704:Analysis of Variance; D000818:Animals; D001672:Biocompatible Materials / Q000633:toxicity*; D002463:Cell Membrane Permeability / Q000187:drug effects; D002470:Cell Survival; D004396:Coloring Agents; D051379:Mice; D009499:Neutral Red; D012387:Root Canal Filling Materials / Q000633:toxicity*
chemical_list: D001672:Biocompatible Materials; C097428:Bioseal; D004396:Coloring Agents; D012387:Root Canal Filling Materials; D009499:Neutral Red