title: The interaction of metal ions and Marimastat with matrix metalloproteinase 9.
abstract: The effect of a range of metal ions on the ability of Marimastat to inhibit matrix metalloproteinase 9 (MMP-9) was examined in a fluorescence based proteolytic assay. Whilst none of the metals examined significantly affected the inhibitory ability of Marimastat, several metal ions did have a significant effect on MMP-9 activity itself. In the absence of Marimastat, Zn(II) and Fe(II) significantly inhibited MMP-9 activity at metal ion concentrations of 10 and 100 microM, respectively. In both the absence and presence of Marimastat, Cd(II) significantly inhibited MMP-9 at 100 microM. In contrast, 1 mM Co(II) significantly upregulated MMP-9 proteolytic activity.
authors.affiliation: Centre for Heavy Metals Research, School of Chemistry, University of Sydney NSW, 2006 Australia.
authors.forename: C K
authors.initials: CK
authors.lastname: Underwood
authors.forename: D
authors.initials: D
authors.lastname: Min
authors.forename: J G
authors.initials: JG
authors.lastname: Lyons
authors.forename: T W
authors.initials: TW
authors.lastname: Hambley
mesh_terms: D004347:Drug Interactions; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D006877:Hydroxamic Acids / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D007951:Leukemia, Myeloid; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D061965:Matrix Metalloproteinase Inhibitors; D019216:Metals, Heavy / Q000737:chemistry* / Q000494:pharmacology*; D009000:Monocytes / Q000166:cytology; D013050:Spectrometry, Fluorescence / Q000379:methods; D014357:Trypsin / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D004791:Enzyme Inhibitors; D006877:Hydroxamic Acids; D061965:Matrix Metalloproteinase Inhibitors; D019216:Metals, Heavy; C100342:marimastat; D014357:Trypsin; D020780:Matrix Metalloproteinase 9