title: [Mutation of 5'noncoding region of the bcl-6 gene in diffuse large B cell lymphomas].
abstract: OBJECTIVE
  To observe the mutation of 5'noncoding region of bcl-6 gene in diffuse large B cell lymphoma (DLBCL) and its effect on lymphoma pathogenesis.
  
  
  METHODS
  38 DLBCL, 2 reactive hyperplasias, 5 follicular lymphomas and 5 T cell lymphomas were chosen for PCR direct sequence analysis using two sets of primers in 5'noncoding region of the bcl-6 gene.
  
  
  RESULTS
  No mutation was found in the marginal region of reactive hyperplasias, T cell lymphomas, and follicular lymphomas but detected in 1/2 of the follicular center cells, and 7/38 cases of DLBCL. The incidence is less than that seen in other reports. Basepairs substitution and point insertion were the main mutation types.
  
  
  CONCLUSIONS
  The positive rate of mutation of 5'noncoding region of bcl-6 gene in DLBCL is 18.7%, less frequent than the published data of DLBCL reported in other countries. It may, in some extent, participate in the pathogenesis and progression of DLBCL.
authors.affiliation: Laboratory of Molecular Pathology, Cancer Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Zhou
authors.forename: Wei-ping
authors.initials: WP
authors.lastname: Zhu
authors.forename: Tai-ming
authors.initials: TM
authors.lastname: Zhang
authors.forename: Xiao-mei
authors.initials: XM
authors.lastname: Li
authors.forename: Ai-ping
authors.initials: AP
authors.lastname: Jin
authors.forename: Meng-hong
authors.initials: MH
authors.lastname: Sun
authors.forename: Xiong-zeng
authors.initials: XZ
authors.lastname: Zhu
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics*; D044466:Asian People; D002681:China; D004268:DNA-Binding Proteins / Q000235:genetics*; D006801:Humans; D016393:Lymphoma, B-Cell / Q000235:genetics*; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics*; D017354:Point Mutation / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D051560:Proto-Oncogene Proteins c-bcl-6; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D020121:5' Untranslated Regions; D004268:DNA-Binding Proteins; D011518:Proto-Oncogene Proteins; D051560:Proto-Oncogene Proteins c-bcl-6; D014157:Transcription Factors