Demonstration of glucose-6-phosphate dehydrogenase in rat Kupffer cells by a newly-developed ultrastructural enzyme-cytochemistry.
Although various tissue macrophages possess high glucose-6-phosphate dehydrogenase (G6PD) activity, which is reported to be closely associated with their phagocytotic/bactericidal function, the fine subcellular localization of this enzyme in liver resident macrophages (Kupffer cells) has not been determined. We have investigated the subcellular localization of G6PD in Kupffer cells in rat liver, using a newly developed enzyme-cytochemical (copper-ferrocyanide) method. Electron-dense precipitates indicating G6PD activity were clearly visible in the cytoplasm and on the cytosolic side of the endoplasmic reticulum of Kupffer cells. Cytochemical controls ensured specific detection of the enzymatic activity. Rat Kupffer cells abundantly possessed enzyme-cytochemically detectable G6PD activity. Kupffer cell G6PD may play a role in liver defense by delivering NADPH to NADPH-dependent enzymes. G6PD enzyme-cytochemistry may be a useful tool for the study of Kupffer cell functions.
Matsubara
S
S
Department of Obstetrics, Gynecology and Anatomy, Jichi Medical School, Minamikawachi-machi 3311-1, Kawachi-gun, Tochigi 329-0498, Japan. matsushi@jichi.ac.jp
Matsubara
D
D
Ishibashi
T
T
Takizawa
T
T
D000818:Animals; D003593:Cytoplasm / Q000201:enzymology / Q000648:ultrastructure; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D006651:Histocytochemistry / Q000379:methods*; D007728:Kupffer Cells / Q000201:enzymology* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D017207:Rats, Sprague-Dawley
D005954:Glucosephosphate Dehydrogenase