[Determination of ursolic acid in herba of Verbena officinalis by HPLC].
OBJECTIVE
To establish a HPLC method for determination of ursolic acid in dried aerial part of Verbena officinalis.


METHODS
The column used was a Kromasil C18 (4.6 mm x 250 mm) packed with a 5 microns stationary phase. The mobile phase consisted of methanol-sodium phosphate buffer [monobasic sodium phosphate (MW = 119.98) 1.7997 g and phosphoric acid (85%) 1.02 mL, combined and brought the total volume of 1,000 mL with water] (89:11); the mobile phase was maintained at a flow-rate of 0.8 mL per minute; the column was maintained at 40 degrees C; the DAD detector was set at 210 nm.


RESULTS
The liner range was 0.251-10.04 micrograms (r = 1.0000). An average recovery of 98.1% (n = 6) was obtained with a RSD of 1.0%.


CONCLUSIONS
The method is simple, accurate and suitable for the qualify control.
Liu
Cheng-hong
CH
Institute for Drug and Instrument Control of PLA, Beijing 100071, China. liuchenghong@chinaren.net
Liu
Yun
Y
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D035261:Plant Components, Aerial / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D014315:Triterpenes / Q000032:analysis*; D032412:Verbena / Q000737:chemistry*; D000097245:Ursolic Acid
D014315:Triterpenes; D000097245:Ursolic Acid