Overlap of promoter and coding sequences in the mouse STOP gene (Mtap6).
The microtubule-associated proteins STOP are essential for synaptic plasticity and integrated brain function. The STOP gene (Mtap6) is composed of 4 exons and presents different developmental and tissue specific alternative transcripts resulting in the neuronal isoforms E- and N-STOP, and in the fibroblastic F-STOP isoform. We now characterize the transcription initiation sites for neuronal and non neuronal STOP transcripts. Our results show that there is a single neuronal specific promoter for transcription of E- and N-STOP mRNAs. F-STOP mRNA transcription is regulated by a distinct promoter. A remarkable feature of this promoter is that it lies within coding sequences expressed in neuronal E- and N-STOP isoforms.
Aguezzoul
Mustapha
M
Laboratoire du Cytosquelette, INSERM U366, Départment Réponse et Dynamique Cellulaires, CEA-Grenoble, 17 rue des Martyrs, 38054 Grenoble, Cedex 9, France.
Andrieux
Annie
A
Denarier
Eric
E
D000818:Animals; D001483:Base Sequence; D003062:Codon; D051379:Mice / Q000235:genetics*; D008869:Microtubule-Associated Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011401:Promoter Regions, Genetic; D020033:Protein Isoforms; D014018:Tissue Distribution; D024363:Transcription Initiation Site
D003062:Codon; D008869:Microtubule-Associated Proteins; C491893:Mtap6 protein, mouse; D020033:Protein Isoforms