Phylogeny of isolates of Prunus necrotic ringspot virus from the Ilarvirus Ringtest and identification of group-specific features.
Isolates of Prunus necrotic ringspot virus (PNRSV) were examined to establish the level of naturally occurring sequence variation in the coat protein (CP) gene and to identify group-specific genome features that may prove valuable for the generation of diagnostic reagents. Phylogenetic analysis of a 452 bp sequence of 68 virus isolates, 20 obtained from the European Union Ilarvirus Ringtest held in October 1998, confirmed the clustering of the isolates into three distinct groups. Although no correlation was found between the sequence and host or geographic origin, there was a general trend for severe isolates to cluster into one group. Group-specific features have been identified for discrimination between virus strains.
Hammond
R W
RW
U.S. Department of Agriculture, Molecular Plant Pathology Laboratory, Beltsville, Maryland 20705, USA. hammondr@ba.ars.usda.gov
D001483:Base Sequence; D005062:European Union; D014644:Genetic Variation; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D010942:Plant Viruses / Q000737:chemistry / Q000145:classification* / Q000235:genetics*; D011110:Polymorphism, Genetic; D014764:Viral Proteins / Q000235:genetics
D014764:Viral Proteins