title: Pti1p and Ref2p found in association with the mRNA 3' end formation complex direct snoRNA maturation.
abstract: Eukaryotic RNA polymerase II transcribes precursors of mRNAs and of non-protein-coding RNAs such as snRNAs and snoRNAs. These RNAs have to be processed at their 3' ends to be functional. mRNAs are matured by cleavage and polyadenylation that require a well-characterized protein complex. Small RNAs are also subject to 3' end cleavage but are not polyadenylated. Here we show that two newly identified proteins, Pti1p and Ref2p, although they were found associated with the pre-mRNA 3' end processing complex, are essential for yeast snoRNA 3' end maturation. We also provide evidence that Pti1p probably acts by uncoupling cleavage and polyadenylation, and functions in coordination with the Nrd1p-dependent pathway for 3' end formation of non-polyadenylated transcripts.
authors.affiliation: Institut de Biochimie et Génétique Cellulaires, CNRS, UMR 5095, 1 Rue Camille Saint Saëns, 33077 Bordeaux cedex, France.
authors.forename: Sonia
authors.initials: S
authors.lastname: Dheur
authors.forename: Le Thuy Anh
authors.initials: le TA
authors.lastname: Vo
authors.forename: Florence
authors.initials: F
authors.lastname: Voisinet-Hakil
authors.forename: Michèle
authors.initials: M
authors.lastname: Minet
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Schmitter
authors.forename: François
authors.initials: F
authors.lastname: Lacroute
authors.forename: Françoise
authors.initials: F
authors.lastname: Wyers
authors.forename: Lionel
authors.initials: L
authors.lastname: Minvielle-Sebastia
mesh_terms: D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005800:Genes, Fungal; D009154:Mutation; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D020537:RNA, Small Nucleolar / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D013489:Suppression, Genetic; D013696:Temperature; D039221:mRNA Cleavage and Polyadenylation Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; C491211:NRD1 protein, S cerevisiae; C490782:PTI1 protein, S cerevisiae; D012331:RNA, Fungal; D012333:RNA, Messenger; D020537:RNA, Small Nucleolar; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; D029701:Saccharomyces cerevisiae Proteins; D039221:mRNA Cleavage and Polyadenylation Factors; D017346:Protein Serine-Threonine Kinases