title: The causes of and cures for contact lens-induced peripheral ulcer.
abstract: OBJECTIVE
  Contact lens-induced peripheral ulceration (CLPU) is a relatively common adverse response associated with wearing hydrogel lenses, especially on an extended wear schedule. Bacteriologic examination of lenses at the time of an event has demonstrated an association with Staphylococci spp. We sought to investigate the causes of CLPU in a rabbit model of contact lens wear.
  
  
  METHODS
  Rabbits wore contact lenses for a period of 24 hr in the presence or absence of bacteria or in presence or absence of epithelial scratches made in the periphery of the cornea before lens wear. Bacteria tested were a strain of Staphylococcus aureus or a strain of Staphylococcus epidermidis isolated from human CLPUs. Rabbits were also challenged with S. aureus in the presence of an epithelial defect and in the absence of a contact lens. Corneas were monitored by slitlamp, histology, and microbial culture after 24 hr.
  
  
  RESULTS
  No CLPU-like lesions were detected under the following conditions: corneal scratch plus lens wear with no bacteria; corneal scratch plus S. epidermidis colonized lenses; corneal scratch without lens wear and with S. aureus applied to eyes; no scratch plus S. aureus colonized lenses; and corneal scratch plus contact lenses colonized by dead S. aureus. CLPU-like lesions were found only when the corneas were scratched and contact lenses colonized by viable S. aureus were applied to the eye. The histology of the lesions demonstrated a frank epithelial break with underlying stromal infiltration. Only low numbers of bacteria could be cultivated from the corneas with CLPU-like lesions. The CLPU-like lesions shared many similarities with CLPU in humans.
  
  
  CONCLUSIONS
  CLPU-like lesions were only produced by S. aureus and not S. epidermidis in the presence of an epithelial abrasion and contact lens. Thus, we hypothesize that to reduce the incidence of CLPU, contact lenses designed to reduce corneal interaction and repel microbial colonization should be produced.
authors.affiliation: Cooperative Research Centre for Eye Research and Technology, University of New South Wales, Sydney, Australia.
authors.forename: P
authors.initials: P
authors.lastname: Wu
authors.forename: Fiona
authors.initials: F
authors.lastname: Stapleton
authors.forename: M D P
authors.initials: MD
authors.lastname: Willcox
mesh_terms: D000818:Animals; D015169:Colony Count, Microbial; D003261:Contact Lenses / Q000009:adverse effects* / Q000382:microbiology; D003319:Corneal Stroma / Q000382:microbiology / Q000473:pathology; D003320:Corneal Ulcer / Q000209:etiology* / Q000517:prevention & control; D019573:Epithelium, Corneal / Q000382:microbiology / Q000473:pathology; D015818:Eye Infections, Bacterial / Q000150:complications* / Q000517:prevention & control; D011817:Rabbits; D013203:Staphylococcal Infections / Q000150:complications* / Q000517:prevention & control; D013211:Staphylococcus aureus / Q000302:isolation & purification / Q000502:physiology*; D013212:Staphylococcus epidermidis / Q000302:isolation & purification / Q000502:physiology*