Localized irregularities in hemoglobin flow and oxygenation in calf muscle in patients with peripheral vascular disease detected with near-infrared spectrophotometry.
OBJECTIVE
Near-infrared spectrophotometry is used to measure flow, concentration, and oxygenation of hemoglobin in arterioles, capillaries, and venules several centimeters deep in tissue. The purpose of this study was to investigate the distribution of flow, concentration, and oxygenation of hemoglobin in calf muscle in patients with documented peripheral arterial occlusive disease (PVD), patients with risk factors for PVD,and healthy younger subjects at rest.


METHODS
With a frequency-domain near-infrared spectrophotometer and a specially designed probe, we generated maps at 22 locations simultaneously of hemoglobin flow, concentration, and oxygenation, with the venous occlusion method. Eight legs of 7 patients with diagnosed PVD (PVD group), 10 legs of 8 patients with normal ankle-brachial index but with risk factors for PVD (RF group), and 16 legs of 8 healthy subjects (H group) were studied.


RESULTS
Global mean values were significantly (P <.05) different between the three groups for oxygen consumption (PVD group, 0.027 +/- 0.009 mL/100 g/min; RF group, 0.038 +/- 0.017 mL/100 g/min; H group, 0.022 +/- 0.020 mL/100 g/min), venous oxygen saturation (PVD, 59.7% +/- 15.4%; RF, 69.6% +/- 10.5%; H, 80.8% +/- 4.5%), and, at 60 s of venous occlusion, concentration changes in oxyhemoglobin (PVD, 4.48 +/- 3.25 micromol/L; RF, 8.44 +/- 2.33 micromol/L; H, 6.85 +/- 4.57 micromol/L), deoxyhemoglobin (PVD, 3.60 +/- 0.73 micromol/L; RF, 4.39 +/- 1.30 micromol/L; H, 2.36 +/- 1.79 micromol/L), and total hemoglobin (PVD, 8.07 +/- 3.83 micromol/L; RF, 12.83 +/- 2.75 micromol/L; H, 9.21 +/- 6.34 micromol/L). No significant difference was found between the three groups for hemoglobin flow (PVD, 0.92 +/- 0.69 micromol/100 mL/min; RF, 1.68 +/- 0.50 micromol/100 mL/min; H, 1.44 +/- 1.17 micromol/100 mL/min) and blood flow (PVD, 0.45 +/- 0.28 mL/100 g/min; RF, 0.77 +/- 0.21 mL/100 g/min; H, 0.62 +/- 0.50 mL/100 g/min). All parameters featured a distribution dependent on location.


CONCLUSIONS
Mean value for venous oxygen saturation was higher in healthy subjects compared to patients with documented PVD. In patients with PVD, areas of lower oxygenation were clearly discernible. At distal locations of calf muscle, significant correlations between reduced hemoglobin flow, venous oxygen saturation, oxyhemoglobin, and total hemoglobin and reduced ankle-brachial index were found. Maps revealed localized irregularities in oxyhemoglobin, total hemoglobin, and venous oxygen saturation in patients with PVD. Near-infrared spectrophotometry is a noninvasive bedside technique that can enable determination of blood flow and oxygenation in tissue and may provide a method for evaluating patients with PVD.
Wolf
Ursula
U
Laboratory for Fluorescence Dynamics, Department of Physics, University of Illinois at Urbana-Champaign, USA.
Wolf
Martin
M
Choi
Jee H
JH
Levi
Moshe
M
Choudhury
Devasmita
D
Hull
Sherri
S
Coussirat
Daniel
D
Paunescu
L Adelina
LA
Safonova
Larisa P
LP
Michalos
Antonios
A
Mantulin
William W
WW
Gratton
Enrico
E
D000328:Adult; D000368:Aged; D000842:Ankle / Q000098:blood supply / Q000502:physiology; D001132:Arm / Q000098:blood supply / Q000502:physiology; D015415:Biomarkers / Q000097:blood; D001794:Blood Pressure / Q000502:physiology; D001916:Brachial Artery / Q000502:physiology; D005260:Female; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009132:Muscles / Q000378:metabolism* / Q000503:physiopathology; D015588:Observer Variation; D010100:Oxygen / Q000097:blood*; D010101:Oxygen Consumption / Q000502:physiology; D016491:Peripheral Vascular Diseases / Q000097:blood* / Q000175:diagnosis* / Q000503:physiopathology; D012039:Regional Blood Flow / Q000502:physiology; D012307:Risk Factors; D013055:Spectrophotometry, Infrared; D013223:Statistics as Topic; D016909:Tibial Arteries / Q000502:physiology
D015415:Biomarkers; D006454:Hemoglobins; D010100:Oxygen