Differentiation of Borrelia burgdorferi sensu lato through groEL gene analysis.
The nucleotide sequences (310 bp) of the groEL gene, which encode the 60-kDa heat shock protein GroEL from 31 reference strains of Borrelia were determined and compared. More than 92.3% similarity was observed among Borrelia burgdorferi sensu lato strains. In the phylogenetic tree constructed with the maximum-likelihood method, each species of B. burgdorferi sensu lato was differentiated as a distinct entity. We developed polymerase chain reaction-restriction fragment length polymorphism analysis using a specific single amino acid variation [N(213) (AAT)-->S (AGC or AGT)] between B. burgdorferi sensu stricto strains and the other B. burgdorferi sensu lato strains. These results showed that the groEL gene is useful for differentiation of B. burgdorferi sensu lato.
Lee
Seung-Hyun
SH
Department of Microbiology, College of Medicine, Konkuk University, 322 Danwol-dong, Chugju, Chungchungbuk-Do 380-701, South Korea.
Lee
Jung-Hee
JH
Park
Hyo-Soon
HS
Jang
Won-Jong
WJ
Koh
Seong-Eun
SE
Yang
Young-Mok
YM
Kim
Bum-Joon
BJ
Kook
Yoon-Hoh
YH
Park
Kyung-Hee
KH
D000595:Amino Acid Sequence; D001483:Base Sequence; D025065:Borrelia burgdorferi / Q000145:classification* / Q000235:genetics* / Q000254:growth & development; D018834:Chaperonin 60 / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA
D018834:Chaperonin 60; D004269:DNA, Bacterial