Improved resolution with microchip-based enhanced field inversion electrophoresis.
We present an improvement of the field inversion electrophoresis (FIE) method in which the passage of sample such as DNA back and forth within a short length of a microchannel can provide a similar resolution to that of a significantly longer microchannel. In constant field FIE the application of an alternating potential (e.g., +/- V) over short periods of time (e.g., several Hz) can provide enhanced separations of DNA fragments. In contrast, the present method consists of a series of separations, each of much longer duration, under high and low fields in such a way that the resolution is enhanced. This method is readily modeled and allows improved resolution to be obtained from extremely short microchannels (e.g., 8 mm) while requiring relatively low applied voltages (e.g., less than 600 V). An additional advantage is that this method can allow for the same equipment to be used in a rapid, low-resolution mode or in a slower, high-resolution mode through what might be referred to as an automated "zoom" capability. We believe that this method may facilitate the integration of microfluidic devices and microelectronic devices by allowing these devices to be of a similar small scale (< 1 cm).
Backhouse
Christopher J
CJ
Department of Electrical and Computer Engineering, University of Alberta, Canada. chrisb@ee.ualberta.ca
Gajdal
Ania
A
Pilarski
Linda M
LM
Crabtree
H John
HJ
D004247:DNA / Q000302:isolation & purification; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods / Q000592:standards; D016521:Electrophoresis, Gel, Pulsed-Field / Q000295:instrumentation* / Q000592:standards; D044085:Microfluidics / Q000295:instrumentation*; D008904:Miniaturization
D004247:DNA