title: Importance of transmembrane segment M3 of Na+,K+-ATPase for control of conformational changes and the cytoplasmic entry pathway for Na.
abstract: A series of mutations were introduced into the sequence Glu(282)-Ile-Glu-His-Phe-Ile-His(288) of the NH(2)-terminal part of M3 of the rat kidney Na(+),K(+)-ATPase, and the resulting mutant pumps were analyzed functionally. Several of the mutations affected the conformational transitions between E(1) and E(2) forms of dephospho- and phosphoenzyme. Mutations to Glu(282) and Phe(286) affected the E(1)-E(2) and E(1)P-E(2)P equilibria in parallel, indicating a role for these two residues in both conformational changes. Mutation to His(285) preferentially affected the E(1)P-E(2)P equilibrium, and mutation to Ile(283) affected only the E(1)-E(2) equilibrium of the dephosphoenzyme, demonstrating that the conformational changes of M3 in the phospho- and dephospho-forms are not identical. Several of the mutants showed a reduced apparent affinity for Na(+), pointing to an important role of the region in optimizing the Na(+) binding properties of the enzyme. It is possible that this part of M3 is closely associated with an entry pathway through which the Na(+) ions pass from the cytoplasmic surface to reach the cation-binding pocket. Some of the mutants also displayed an increased Na(+)-ATPase activity, and a good correlation was observed between the turnover rate for Na(+)-ATPase activity and the rate of dephosphorylation in the absence of K(+), indicating an increased ability of Na(+) to activate dephosphorylation of E(2)P by binding in place of K(+) at the extracellularly facing sites. Thus, M3 also seems to be a part of the signaling pathway between the external cation sites and the catalytic site.
authors.affiliation: Department of Physiology, University of Aarhus, Denmark. bv@fi.au.dk
authors.forename: Bente
authors.initials: B
authors.lastname: Vilsen
authors.forename: Mads
authors.initials: M
authors.lastname: Toustrup-Jensen
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D001665:Binding Sites; D001693:Biological Transport, Active; D020134:Catalytic Domain; D002462:Cell Membrane / Q000201:enzymology*; D003593:Cytoplasm / Q000201:enzymology; D007700:Kinetics; D010766:Phosphorylation; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012964:Sodium / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000737:chemistry* / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase