In situ photoaffinity labeling of the target protein for lembehyne A, a neuronal differentiation inducer.
A C(36) linear acetylene alcohol, lembehyne A (LB-A), induces neuronal differentiation against neuroblastoma cells morphologically and also functionally. The differentiation and cytostatic effect induced by LB-A was specific to neuroblastoma, Neuro 2A cells. To identify the target protein for LB-A, a radioactive photoaffinity probe, [(125)I]18-(2'-azido-5'-iodo-benzoyloxy)-LB-18 ([(125)I]azido-LB-18), was synthesized. As a result of in situ labeling experiments against Neuro 2A cells, a protein of M(r) 30 kDa was photolabeled specifically. This labeling was inhibited in the presence of LB-A or the active analogs of LB-A, whereas the inactive analogs showed no inhibitory effect on this labeling. These results suggest that this protein of M(r) 30 kDa is the target protein for LB-A and may play an important role for the neuronal differentiation in neuroblastoma, Neuro 2A cells.
Aoki
Shunji
S
Graduate School of Pharmaceutical Sciences, Osaka University, Yamada-oka 1-6, Suita, Japan.
Matsui
Kouhei
K
Takata
Tetsuo
T
Kobayashi
Motomasa
M
D000480:Alkynes / Q000737:chemistry* / Q000494:pharmacology*; D000818:Animals; D015153:Blotting, Western; D002453:Cell Cycle; D002454:Cell Differentiation; D004591:Electrophoresis, Polyacrylamide Gel; D005233:Fatty Alcohols / Q000737:chemistry* / Q000494:pharmacology*; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D020014:K562 Cells; D008027:Light; D051379:Mice; D008956:Models, Chemical; D009474:Neurons / Q000166:cytology*; D011161:Porifera; D051381:Rats; D014407:Tumor Cells, Cultured
D000480:Alkynes; D005233:Fatty Alcohols; C444641:lembehyne A