Production of verbenol, a high valued food flavourant from a fusant strain of Aspergillus niger.
A hyperperformer for the production of verbenol was produced from the fusion of two improved strains of Aspergillus niger. A 2-deoxy glucose de-repressed mutant [high sporulation (50%), viability (80%) showing a conversion of 15.6% of initial alpha-pinene to verbenol in 6 h under the conditions used] was fused with another strain enriched with alpha-pinene (26.4% of alpha pinene converted to verbenol) to obtain a final verbenol conversion yield of 48.6% of initial alpha pinene.
Vidya
C M
CM
Department of Food Microbiology, Central Food Technological Research Institute, 570 023, Mysore, India.
Agrawal
R
R
D001234:Aspergillus niger / Q000235:genetics* / Q000378:metabolism; D000081008:Bicyclic Monoterpenes; D001709:Biotechnology / Q000379:methods; D005421:Flavoring Agents / Q000378:metabolism*; D039821:Monoterpenes / Q000378:metabolism*; D009154:Mutation; D011993:Recombinant Fusion Proteins / Q000378:metabolism*
D000081008:Bicyclic Monoterpenes; D005421:Flavoring Agents; D039821:Monoterpenes; D011993:Recombinant Fusion Proteins; C009951:verbenol; C005451:alpha-pinene