The role of the TSC-22 (-396) A/G variant in the development of diabetic nephropathy.
TSC-22 is a leucine zipper transcriptional factor and expression of the TSC-22 gene is highly induced by TGF-beta treatment. We estimated the frequency of the -396 A/G polymorphism of the TSC-22 gene with an Alu I-Restriction fragment length polymorphism (RFLP) method in 498 Japanese subjects with type 2 diabetes mellitus. We also determined the promoter activity. The diabetic patients with the AA genotype had a significantly higher incidence of the diabetic nephropathy (vs. the AG genotype, P<0.05, odds ratio: 1.95; 95% confidence intervals 1.14-3.33). There was no significant difference in the promoter activity between the fragments with -396A and -396G. These findings suggest that the TSC-22 gene (-396) A allele is associated with an increasing risk of the diabetic nephropathy.
Sugawara
Fumiaki
F
Department of Diabetes and Clinical Nutrition, Graduate School of Medicine, Kyoto University, 54 Shogoin-Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan. sugawara@metab.kuhp.kyoto-u.ac.jp
Yamada
Yuichiro
Y
Watanabe
Rie
R
Ban
Nobuhiro
N
Miyawaki
Kazumasa
K
Kuroe
Akira
A
Hamasaki
Akihiro
A
Ikeda
Hiroki
H
Kurose
Takeshi
T
Usami
Masaru
M
Ikeda
Masaki
M
Seino
Yutaka
Y
D045744:Cell Line, Tumor; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D003928:Diabetic Nephropathies / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D016015:Logistic Models; D008156:Luciferases / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins; D014157:Transcription Factors / Q000235:genetics*; D016212:Transforming Growth Factor beta
D012097:Repressor Proteins; C099726:TSC22D1 protein, human; D014157:Transcription Factors; D016212:Transforming Growth Factor beta; D008156:Luciferases