Effect of oocyte quality on the relative abundance of specific gene transcripts in bovine mature oocytes and 16-cell embryos.
Although the developmental potential of oocytes is related to oocyte quality, whether the expression of specific genes is altered in oocytes of different quality and in resulting embryos is not known. Semi-quantitativereverse transcription-polymerase chain reaction was used to compare the relative abundance of 2 transcripts for housekeeping proteins (beta-actin and ribosomal protein L30) and 3 transcripts for growth factor ligand or receptors (platelet derived growth factor receptor alpha (PDGFRalpha), basic fibroblast growth factor (bFGF)), in mature bovine oocytes of high versus low developmental potential. The transcripts for L30, PDGFRalpha, and bFGF in 16-cell embryos originating from these oocytes were also examined. No significant effect of oocyte quality was detected for any of the transcripts examined from oocytes or 16-cell embryos. In conclusion, a lower developmental potential of oocytes with advanced signs of atresia, was not associated with a lower level of abundance of the transcripts examined.
Bilodeau-Goeseels
Sylvie
S
Agriculture and Agri-Food Canada, Lethbridge Research Centre, P.O. Box 3000, Lethbridge, Alberta T1J 4B1. goeseelss@agr.gc.ca
D000199:Actins / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000196:embryology* / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006133:Growth Substances / Q000235:genetics* / Q000378:metabolism; D008297:Male; D009865:Oocytes / Q000378:metabolism / Q000502:physiology*; D011247:Pregnancy; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000235:genetics / Q000378:metabolism; D017978:Receptors, Growth Factor / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D012269:Ribosomal Proteins / Q000235:genetics*; D014158:Transcription, Genetic
D000199:Actins; D018076:DNA, Complementary; D006133:Growth Substances; D017978:Receptors, Growth Factor; D012269:Ribosomal Proteins; C035201:ribosomal protein L30; D016222:Fibroblast Growth Factor 2; D012313:RNA; D020796:Receptor, Platelet-Derived Growth Factor alpha