title: Transposition in Lactobacillus delbrueckii subsp. bulgaricus: identification of two thermosensitive replicons and two functional insertion sequences.
abstract: In this report, it is shown that the rolling circle replicon pG(+)host and the theta replicon pIP501 are thermosensitive in Lactobacillus delbrueckii subsp. bulgaricus (Lactobacillus bulgaricus). Using a pIP501 derivative as a delivery vector for six insertion sequences originating from lactic acid bacteria, it is shown that IS1223 and IS1201 transpose in L. bulgaricus.
authors.affiliation: Unité Recherche Laitière et Génétique Appliquée, INRA, Domaine de Vilvert, 78352 Jouy-en-Josas Cedex, France.
authors.forename: Pascale
authors.initials: P
authors.lastname: Serror
authors.affiliation: Génétique Microbienne, INRA, Domaine de Vilvert, 78352 Jouy-en-Josas Cedex, France.
authors.forename: Golnar
authors.initials: G
authors.lastname: Ilami
authors.affiliation: Génétique Microbienne, INRA, Domaine de Vilvert, 78352 Jouy-en-Josas Cedex, France.
authors.forename: Hichem
authors.initials: H
authors.lastname: Chouayekh
authors.affiliation: Génétique Microbienne, INRA, Domaine de Vilvert, 78352 Jouy-en-Josas Cedex, France.
authors.forename: S Dusko
authors.initials: SD
authors.lastname: Ehrlich
authors.affiliation: Génétique Microbienne, INRA, Domaine de Vilvert, 78352 Jouy-en-Josas Cedex, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Maguin
mesh_terms: D001483:Base Sequence; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D007778:Lactobacillus / Q000235:genetics*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000379:methods; D010957:Plasmids / Q000235:genetics; D012093:Replicon; D013696:Temperature
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial