title: [Determination of salvianolic acid B in the radix of Salvia miltiorrhiza Bge. by HPLC].
abstract: OBJECTIVE
  To develop an HPLC method for determining salvianolic acid B in radix of Salvia miltiorrhiza.
  
  
  METHODS
  The sample was extracted with 75% methanol. ODS column was used with methanol-5% acetic acid solution (35:65) as mobile phase. Detection wavelength was 281 nm.
  
  
  RESULTS
  Salvianolic acid B was separated well. Good linearity of salvianolic acid B was obtained (r = 0.9997) within the range of 0.17-1.7 micrograms. The average recovery was 98.9%. Repeatability was good, and RSD was 1.82%.
  
  
  CONCLUSIONS
  This method can be used for quality control of radix of Salvia miltiorrhiza.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of TCM, Beijing 100700, China.
authors.forename: Q W
authors.initials: QW
authors.lastname: Zhang
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: J P
authors.initials: JP
authors.lastname: Li
authors.forename: H
authors.initials: H
authors.lastname: Ma
mesh_terms: D001572:Benzofurans / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D053630:Depsides
chemical_list: D001572:Benzofurans; C076944:salvianolic acid B; D053630:Depsides