title: Simultaneous determination of sildenafil and its active metabolite UK-103,320 in human plasma using liquid chromatography-tandem mass spectrometry.
abstract: A liquid chromatography-tandem mass spectrometric method for the simultaneous determination of sildenafil and its active N-demethylated metabolite, UK-103,320 in human plasma was developed. Sildenafil, UK-103,320 and the internal standard (DA-8159) were extracted from human plasma with dichloromethane at basic pH. A reverse-phase LC separation was performed on Luna phenylhexyl column with the mixture of acetonitrile-ammonium formate (10 mM, pH 6.0) (60:40, v/v) as mobile phase. The detection of analytes was performed using an electrospray ionization tandem mass spectrometry in the multiple reaction-monitoring mode. The lower limits of quantification for sildenafil and UK-103,320 were 2.0 ng/ml. The method showed a satisfactory sensitivity, precision, accuracy, recovery and selectivity.
authors.affiliation: Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, BC, Canada V6T 1Z3.
authors.forename: John
authors.initials: J
authors.lastname: Kim
authors.forename: HyeYoung
authors.initials: H
authors.lastname: Ji
authors.forename: Sook Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Hye Won
authors.initials: HW
authors.lastname: Lee
authors.forename: Seung-Seok
authors.initials: SS
authors.lastname: Lee
authors.forename: Dong Sung
authors.initials: DS
authors.lastname: Kim
authors.forename: Moohi
authors.initials: M
authors.lastname: Yoo
authors.forename: Won Bae
authors.initials: WB
authors.lastname: Kim
authors.forename: Hye Suk
authors.initials: HS
authors.lastname: Lee
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D010879:Piperazines / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D011687:Purines; D011744:Pyrimidinones / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D000068677:Sildenafil Citrate; D013450:Sulfones
chemical_list: D010879:Piperazines; D011687:Purines; D011744:Pyrimidinones; D013450:Sulfones; C109995:UK 103320; D000068677:Sildenafil Citrate