title: A murine model of cholesteatoma-induced bone resorption using autologous dermal implantation.
abstract: OBJECTIVE
  To investigate a novel murine model for dermal implant-induced osteolysis analogous to bone resorption observed in middle ear cholesteatoma.
  
  
  METHODS
  Animal experiment.
  
  
  METHODS
  We placed autologous dermal implants on the surface of mouse calvaria. The calvaria were examined at days 1, 3, 5, 7, and 14 after implantation by histological study and tartrate-resistant acid phosphatase immunohistochemical processing to detect osteoclasts.
  
  
  RESULTS
  Dermal implants showed a significantly increased osteoclast density compared with control samples. The dermal implant tissue remained viable and produced a robust, localized inflammatory osteolytic response on the adjacent calvarial surface. Osteoclasts were predominantly found on the surface of the calvarium with the greatest osteoclast density seen at 5 to 7 days after implantation.
  
  
  CONCLUSIONS
  The mouse model is expected to be a useful tool for investigating the pathogenesis of localized inflammatory bone resorption related to cholesteatoma.
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, University of Bochum, Germany. Holger.Sudhoff@ruhr-uni-bochum.de
authors.forename: Holger
authors.initials: H
authors.lastname: Sudhoff
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Liebehenz
authors.forename: Jessica
authors.initials: J
authors.lastname: Aschenbrenner
authors.forename: Jae
authors.initials: J
authors.lastname: Jung
authors.forename: Henning
authors.initials: H
authors.lastname: Hildmann
authors.forename: Stefan
authors.initials: S
authors.lastname: Dazert
mesh_terms: D000818:Animals; D001862:Bone Resorption / Q000473:pathology*; D018424:Cholesteatoma, Middle Ear / Q000473:pathology*; D020405:Dermis / Q000637:transplantation; D004195:Disease Models, Animal; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D010010:Osteoclasts / Q000473:pathology; D010014:Osteolysis / Q000473:pathology; D012886:Skull / Q000473:pathology