Lipoteichoic acid up-regulates VEGF expression in macrophages and pulp cells.
Vascular endothelial growth factor (VEGF) is a potent inducer of angiogenesis, vascular permeability, and edema. Up-regulation of VEGF expression in the dental pulp may result in increased intra-pulpal pressure, and contribute to pain and irreversible tissue damage. Lipoteichoic acid (LTA) is an amphiphilic molecule from Gram-positive bacteria that has been associated with the pathogenesis of pulpitis. To investigate if LTA regulates expression of VEGF, we exposed mouse odontoblast-like cells (MDPC-23), undifferentiated pulp cells (OD-21), fibroblasts, or macrophages to streptococcal LTA, and evaluated VEGF expression by ELISA and RT-PCR. LTA induced up to a nine-fold increase in VEGF protein expression in macrophages, a 2.4-fold increase in MDPC-23, and a 1.6-fold increase in OD-21 as compared with controls. In contrast, LTA did not induce VEGF expression in fibroblasts. VEGF mRNA expression remained constant upon exposure to LTA, which suggests that VEGF regulation in these cells is primarily post-transcriptional. This work constitutes the first demonstration that lipoteichoic acid is sufficient to induce expression of a pro-angiogenic factor.
Telles
P D S
PD
Department of Cariology, Restorative Sciences, and Endodontics, University of Michigan School of Dentistry, Ann Arbor, 48109-1078, USA.
Hanks
C T
CT
Machado
M A A M
MA
Nör
J E
JE
D000704:Analysis of Variance; D043925:Angiogenesis Inducing Agents / Q000494:pharmacology; D000818:Animals; D018929:Cell Culture Techniques; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects*; D016228:Endothelial Growth Factors / Q000032:analysis*; D005347:Fibroblasts / Q000187:drug effects; D005881:Gingiva / Q000166:cytology / Q000187:drug effects; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008222:Lymphokines / Q000032:analysis*; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D009804:Odontoblasts / Q000187:drug effects; D020033:Protein Isoforms / Q000032:analysis*; D012323:RNA Processing, Post-Transcriptional / Q000187:drug effects; D012333:RNA, Messenger / Q000032:analysis; D013291:Streptococcus; D013682:Teichoic Acids / Q000494:pharmacology*; D015854:Up-Regulation; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D043925:Angiogenesis Inducing Agents; D016228:Endothelial Growth Factors; D036341:Intercellular Signaling Peptides and Proteins; D008070:Lipopolysaccharides; D008222:Lymphokines; D020033:Protein Isoforms; D012333:RNA, Messenger; D013682:Teichoic Acids; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; C009900:lipoteichoic acid