[Studies on metabolites of baicalin in human urine].
OBJECTIVE
We are interested in the metabolism of baicalin in human body to offer more information to clinic.


METHODS
Urine of ten persons was collected during 2-18 h after taking 1.0 g of baicalin orally, and the urine was concentrated to 0.5 L, then filtered, the solution being loaded on an iron exchange column eluted with 1.0 L of H2O and 1.0 L of 50% EtOH. The EtOH fraction was concentrated and loaded on a Sephadex LH 20 column eluted with 0.5 L of H2O to obtain 15 fractions, which were detected by silica gel TLC (CHCl3-MeOH-H2O, 1:1:0.1). The fractions 8-11 showed flavonoid characteristics by spraying FeCl3 reagent, and the four fractions were combined to obtain the sample containing metabolites of baicalin for HPLC analysis and LCMS measurement.


CONCLUSIONS
M1, M2 and M3 was identified as baicalein-6-O-beta-glucopyranuronoside; 6-O-methyl-baicalin-7-O-beta-glucopyranuronoside and baicalin.
Che
Q M
QM
School of Pharmaceutical Sciences, Peking University, Beijing 100083, China.
Huang
X L
XL
Li
Y M
YM
Kun
Z
Z
Teruaki
A
A
Masao
H
H
D000328:Adult; D044950:Flavanones; D005419:Flavonoids / Q000302:isolation & purification / Q000378:metabolism* / Q000652:urine*; D005965:Glucuronates / Q000652:urine*; D006801:Humans; D008297:Male; D008875:Middle Aged; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D031364:Scutellaria baicalensis / Q000737:chemistry
C477967:6-O-methylbaicalin-7-O-glucopyranuronoside; D044950:Flavanones; D005419:Flavonoids; D005965:Glucuronates; C477966:baicalein-6-O-glucopyranuronoside; C038044:baicalin; C006680:baicalein