title: Influences of dibutyryl cyclic adenosine monophosphate and forskolin on human sperm motility in vitro.
abstract: OBJECTIVE
  To study the influences of dibutyryl cyclic adenosine monophosphate (dbcAMP) and forskolin on human sperm motility in vitro.
  
  
  METHODS
  Semen samples, aseptically obtained by masturbation and prepared by swim-up technique from 20 fertile men, were incubated with different concentrations of dbcAMP and forskolin at 37 deg. Measurements were carried out after 10 min, 20 min, 30 min and 60 min incubation. Motility parameters were estimated by using an automatic analyzing system.
  
  
  RESULTS
  Treatment with dbcAMP or forskolin resulted in a significant increase in sperm motility and progressive motility. The larger the concentrations of dbcAMP or forskolin, the greater the effect appeared. The straight linear velocity and curvilinear velocity were not affected by both agents.
  
  
  CONCLUSIONS
  dbcAMP and forskolin increase the motility and progressive motility of human sperm in vitro.
authors.affiliation: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. jhliu888@hotmail.com
authors.forename: Ji-Hong
authors.initials: JH
authors.lastname: Liu
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Zheng-Guo
authors.initials: ZG
authors.lastname: Cao
authors.forename: Zhang-Qun
authors.initials: ZQ
authors.lastname: Ye
mesh_terms: D000328:Adult; D003994:Bucladesine / Q000008:administration & dosage / Q000494:pharmacology*; D005576:Colforsin / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D009994:Osmolar Concentration; D013081:Sperm Motility / Q000187:drug effects*
chemical_list: D005576:Colforsin; D003994:Bucladesine