Drosophila DSP1 and rat HMGB1 have equivalent DNA binding properties and share a similar secondary fold.
The protein DSP1 belongs to the group of HMG-box proteins, which share the common structural feature of the HMG-box. This approximately 80 amino acid long motif binds DNA via the minor groove. DSP1 was discovered as a transcriptional co-repressor of Dorsal in Drosophila melanogaster and then was shown to participate to the remodeling of chromatin. By means of sequence alignment and gene organization, DSP1 was classified as the fly homologue of the vertebrate proteins HMGB1/2. DSP1 contains two HMG boxes flanked by two glutamine-rich domains at the N-terminus. In addition, the HMG domain of DSP1 displays two differences in its primary sequence as compared to the vertebrate HMGB1: a shorter acidic tail and a linker between the two boxes longer by 6 amino acids. By comparing several functional parameters of DSP1 with those of HMGB1, the present study establishes the functional equivalence of both proteins in terms of DNA recognition. The major structural difference between the two proteins, the glutamine-rich N-terminal tail of DSP1, which does not exist in HMGB1, did not interfere with any of the studied DNA-binding properties of the proteins.
Janke
Carsten
C
Centre de Biophysique Moléculaire, CNRS, conventionné avec l'Université d'Orléans, rue Charles Sadron, 45071 Orléans cedex 2, France.
Martin
Davy
D
Giraud-Panis
Marie-Josèphe
MJ
Decoville
Martine
M
Locker
Daniel
D
D000818:Animals; D002843:Chromatin / Q000235:genetics; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D011088:DNA Ligases / Q000378:metabolism; D029721:Drosophila Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics*; D005973:Glutamine / Q000737:chemistry / Q000235:genetics; D024243:HMGB1 Protein / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D024261:HMGB2 Protein / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006609:High Mobility Group Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics; D014158:Transcription, Genetic
D002843:Chromatin; C104348:DSP1 protein, Drosophila; D029721:Drosophila Proteins; D024243:HMGB1 Protein; D024261:HMGB2 Protein; D006609:High Mobility Group Proteins; D011994:Recombinant Proteins; D005973:Glutamine; D004247:DNA; D011088:DNA Ligases