title: Efficacy of two 65% permethrin spot-on formulations against induced infestations of Ctenocephalides felis (Insecta: Siphonaptera) and Amblyomma americanum (Acari: Ixodidae) on beagles.
abstract: The efficacy of two formulations of a topically applied 65% permethrin spot-on (Defend Exspot Treatment for Dogs, Schering-Plough Animal Health) was evaluated against experimental infestations of the cat flea Ctenocephalides felis and the lone star tick Amblyomma americanum in dogs. Eighteen dogs were randomly assigned to treatment with 65% permethrin in either diethylene glycol monomethyl ether (DGME; original formulation) or propylene glycol monomethyl ether (PGME) or to be untreated as a control. Treated dogs received either 1 (body weight < 15 kg) or 2 ml (body weight > or =15 kg) of the assigned formulation on Day 0. One hundred unfed, adult C. felis were placed on each dog on Days -6, -1, 4, 11, 18, 25, and 32. Fifty unfed, adult ticks were placed on each dog on Days -1, 3, 9, 16, 23, and 30. Live fleas and ticks were counted and removed on Days 3, 7, 14, 21, and 28. Treatment of dogs with the 65% permethrin in DGME reduced flea numbers by 90.4% to 99.9% from Days 3 through 21 (P < or =.05) and by 48.2% 28 days after treatment. Treatment of dogs with 65% permethrin in PGME reduced flea numbers by 93.7% to 99.7% from Days 3 through 28 and by 78.4% 35 days after treatment (P < or =.05). Treatment with 65% permethrin in DGME reduced tick numbers by 90% or more only on Day 7, whereas treatment with 65% permethrin in PGME reduced the number of live ticks by 90%or more on Days 7 and 14 and approached 90%(87.9%) on Day 21. Efficacy against fleas and ticks for the PGME formulation was significantly better (P < or =.05) than for the DGME formulation on Day 28. Findings in this study indicate that both the DGME and PGME formulations of 65% permethrin performed well in reducing numbers of live C. felis and A. americanum on laboratory beagles; however, the PGME formulation was effective approximately 1 to 2 weeks longer than the DGME formulation.
authors.affiliation: Schering Plough Animal Health, 1095 Morris Avenue, Union, NJ 07083, USA.
authors.forename: Richard G
authors.initials: RG
authors.lastname: Endris
authors.forename: J Alexander
authors.initials: JA
authors.lastname: Hair
authors.forename: Gary
authors.initials: G
authors.lastname: Anderson
authors.forename: Wayne B
authors.initials: WB
authors.lastname: Rose
authors.forename: Damon
authors.initials: D
authors.lastname: Disch
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: Meyer
mesh_terms: D000818:Animals; D002626:Chemistry, Pharmaceutical; D004199:Disease Vectors; D004283:Dog Diseases / Q000188:drug therapy* / Q000469:parasitology; D004285:Dogs; D004478:Ectoparasitic Infestations / Q000188:drug therapy* / Q000469:parasitology / Q000662:veterinary*; D005260:Female; D007306:Insecticides / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D026863:Ixodidae / Q000187:drug effects*; D008297:Male; D026023:Permethrin / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D005423:Siphonaptera / Q000187:drug effects*
chemical_list: D007306:Insecticides; D026023:Permethrin