title: The detection and definition of IgM alloantibodies in the presence of IgM autoantibodies using flowPRA beads.
abstract: We have developed a flow cytometry-based screening method using FlowPRA (One Lambda) human leukocyte antigen (HLA) class I panel beads and FlowPRA (One Lambda) HLA class I specificity beads for the detection and definition of immunoglobulin (Ig)M HLA-specific antibodies in the presence of IgM autoantibodies. Forty-six autoantibody-positive patients who were on the waiting list for a renal transplant (56 sera) were tested in parallel with FlowPRA (One Lambda) HLA class I beads and FlowPRA (One Lambda) control beads. Sera that were positive for IgM HLA class I antibodies were subsequently tested with FlowPRA HLA class I specificity beads to determine the HLA specificities. Thirteen of the 46 patients were positive for IgM HLA class I-specific antibodies. Eleven of the 13 had previous failed transplants and 2 were awaiting a primary transplant. For 9 of the 13 positive patients, IgM HLA class I specificities were defined. We have demonstrated the presence of IgM HLA-specific antibodies in patients with IgM autoantibodies. This study demonstrates the value of FlowPRA HLA class I panel and specificity beads for the detection and definition of IgM HLA class I-specific antibodies.
authors.affiliation: Transplantation Laboratory, Manchester Royal Infirmary, Manchester, United Kingdom. Naheed.Khan@cmmc.nhs.uk
authors.forename: Naheed
authors.initials: N
authors.lastname: Khan
authors.forename: Amanda J
authors.initials: AJ
authors.lastname: Robson
authors.forename: Judith E
authors.initials: JE
authors.lastname: Worthington
authors.forename: Susan
authors.initials: S
authors.lastname: Martin
mesh_terms: D001323:Autoantibodies / Q000276:immunology*; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology*; D007518:Isoantibodies / Q000032:analysis* / Q000276:immunology; D016030:Kidney Transplantation / Q000276:immunology; D008863:Microspheres
chemical_list: D001323:Autoantibodies; D007075:Immunoglobulin M; D007518:Isoantibodies