[Detection of human papillomavirus gene in biopsies from colon carcinoma by PCR].
OBJECTIVE
To detect the human papillomavirus gene (HPVs) in biopsies from colon carcinoma by PCR and to study the relationship between HPV infection and colon carcinoma.


METHODS
46 biopsies from colon carcinoma, 10 biopsies from tissue around carcinoma and 16 control biopsies were collected and DNA were extracted. The HPV DNA in biopsies were amplified by PCR using general primers (GP) and primers for HPV6/11, HPV16/33 and HPV18.


RESULTS
20 out of 46 biopsies from colon carcinoma showed HPV gene positive, HPV6/11, HPV6/33 and HPV18 positivity were 3, 11 and 6 respectively. HPV DNA was found in 1 case from 10 biopsies of surrounding tissue of carcinoma. 16 control biopsies were negative.


CONCLUSIONS
HPV may be related to the etiology of colon carcinoma.
Zhu
Q
Q
Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052.
Cao
J
J
Li
S
S
D015179:Colorectal Neoplasms / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D005814:Genes, Viral; D006801:Humans; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000821:virology*; D016133:Polymerase Chain Reaction
D004279:DNA, Viral