title: Flavonoids detection by HPLC-DAD-MS-MS in lemon juices from Sicilian cultivars.
abstract: High-performance liquid chromatography-diode array detector (HPLC-DAD) coupled with electronspray mass spectrometry (ESI-MS) was used to detect the flavonoid profile in lemon juices obtained from the main Sicilian cultivars (Femminello comune, Monachello, and Interdonato). Significant amounts of an unusual constituent were found in the lemon juice of the above-mentioned cultivars together with eriocitrin, hesperidin, and diosmin. Following purification by preparative HPLC, the structure was assigned as 6,8-di-C-glucopyranosyldiosmetin by means of DAD-UV, ESI-MS-MS, and (1)H NMR analysis. Three other minor components were also detected. One of these presented a flavone nature, and spectral data and literature references both suggested a 6,8-di-C-glucopyranosylapigenin structure. The different contents of eriocitrin, hesperidin, diosmin, and above minor components in the cultivars allow juices to be readily differentiated.
authors.affiliation: Dipartimento di Chimica Organica e Biologica, Università di Messina, Salita Sperone 31, 98166 S. Agata di Messina, Italy. caristi@isengard.unime.it
authors.forename: Corrado
authors.initials: C
authors.lastname: Caristi
authors.forename: Ersilia
authors.initials: E
authors.lastname: Bellocco
authors.forename: Vincenza
authors.initials: V
authors.lastname: Panzera
authors.forename: Giovanni
authors.initials: G
authors.lastname: Toscano
authors.forename: Rosa
authors.initials: R
authors.lastname: Vadalà
authors.forename: Ugo
authors.initials: U
authors.lastname: Leuzzi
mesh_terms: D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002957:Citrus / Q000737:chemistry*; D004145:Diosmin / Q000032:analysis; D044950:Flavanones; D005419:Flavonoids / Q000032:analysis*; D005638:Fruit / Q000737:chemistry; D006569:Hesperidin / Q000031:analogs & derivatives* / Q000032:analysis; D012802:Sicily; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D044950:Flavanones; D005419:Flavonoids; D004145:Diosmin; C114706:eriocitrin; D006569:Hesperidin