Interspecific variation in terpenoid composition of defensive secretions of European Reticulitermes termites.
Sixteen terpene compounds were isolated from the soldier defensive secretions of seven European termite taxa of the genus Reticulitermes (Isoptera, Rhinotermitidae). We describe species-specific mixtures of monoterpenes (alpha-pinene, beta-pinene, limonene), sesquiterpenes (germacrene C, germacrene A, germacrene B, beta-selinene, delta-selinene, gamma-selinene, (E)-beta-farnesene, gamma-cadinene, nerolidol), diterpenes (geranyl linalool, geranyl geraniol, geranyl geranial), and one sesterterpene (geranyl farnesol). Compounds were purified by HPLC and their structures determined by means of MS spectrometry, or 1D and 2D NMR spectroscopy. Comparison of two different analytical approaches, CC-MS and HPLC with subsequent NMR spectroscopy, revealed Cope rearrangement of germacrene A, germacrene B, and germacrene C to the respective beta-elemene, gamma-elemene, and delta-elemene under GC conditions, thus demonstrating the limits for this analytical approach. The species-specific compound composition provides insight into taxonomy and species origin of European Reticulitermes. The biological significance of the species-specific composition of Reticulitermes defensive secretions is briefly discussed.
Quintana
Alexandre
A
CNRS, Laboratoire de Neurobiologie-UPR 9024, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France.
Reinhard
Judith
J
Faure
Robert
R
Uva
Paolo
P
Bagnères
Anne-Geneviève
AG
Massiot
Georges
G
Clément
Jean-Luc
JL
D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D002851:Chromatography, High Pressure Liquid; D005060:Europe; D005088:Exocrine Glands / Q000502:physiology*; D008401:Gas Chromatography-Mass Spectrometry; D020049:Isoptera / Q000145:classification / Q000235:genetics* / Q000502:physiology*; D011157:Population Dynamics; D013045:Species Specificity; D013729:Terpenes / Q000737:chemistry*
D013729:Terpenes