Human bone marrow endothelial cells elaborate non-stromal-cell-derived factor-1 (SDF-1)-dependent chemoattraction and SDF-1-dependent transmigration of haematopoietic progenitors.
This study investigated human bone marrow endothelial cells (BMEC) chemoattractive activity in relation to haematopoietic cell trafficking. BMEC-conditioned medium induced chemoattraction of haematopoietic progenitor cells. Migration was not inhibited by pretreating the cells with pertussis toxin (PTX) or 12G5, indicating that the chemoattractive activity was not dependent on stromal-cell-derived factor-1 (SDF-1). Spontaneous migration, but not SDF-1-mediated chemotaxis of haematopoietic progenitors, was better supported by BMEC as compared with umbilical vein endothelial cells. The superior migration was abolished by pretreating the cells with PTX, indicating that BMEC-derived SDF-1 favours bone marrow endothelium, with better transmigration of haematopoietic progenitors.
Jo
Deog-Yeon
DY
Division of Hematology/Oncology, Department of Internal Medicine, and Department of Pathology, College of Medicine, Chungnam National University, Daejon, Korea. deogyeon@cnu.ac.kr
Hwang
Jin-Hee
JH
Kim
Jin-Man
JM
Yun
Hwan-Jung
HJ
Kim
Samyong
S
D001854:Bone Marrow Cells / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D054377:Chemokine CXCL12; D019743:Chemokines, CXC / Q000502:physiology*; D017077:Culture Media, Conditioned; D004727:Endothelium; D006412:Hematopoietic Stem Cells / Q000502:physiology; D006801:Humans
C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D019743:Chemokines, CXC; D017077:Culture Media, Conditioned