[Construction of adenovirus 4 vector with deletion of 78.9-86 mu fragment and express of beta-galactosidase gene].
OBJECTIVE
To construct a human adenovirus type 4(Ad4) vector with partial deletion at E3 region (78.9-86 mu).


METHODS
Ad4 DNA was extracted from purified virus cultured in WI-38 cells. The essential fragment(71.3-100 mu) covering Ad4 E3 region was cloned and partial deletion of E3 region of this clone has been performed, generating plasmid pAd4 delta KS. Furthermore, a beta-galactosidase (beta-gal) gene flanked by CMV early promoter and SV40 poly A signal was inserted into pAd4 delta KS, resulting in pAd4c beta. This plasmid was cotransfected with Ad4 DNA, Bcl I A fragments into 293 cells, producing a non-defective recombinant Ad4 virus encoding B-gal.


RESULTS
The results showed that the constructed recombinant virus could efficiently express the foreign gene, beta-gal.


CONCLUSIONS
Ad4 vector with a deletion of E3 region can be explored as a live vaccine for prevention of human infectious diseases.
Shi
C
C
Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052.
Liu
S
S
Zhou
W
W
D017503:Adenovirus E3 Proteins / Q000235:genetics; D000260:Adenoviruses, Human / Q000145:classification / Q000235:genetics*; D002460:Cell Line; D004279:DNA, Viral / Q000235:genetics; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D008168:Lung / Q000166:cytology / Q000196:embryology; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics*
D017503:Adenovirus E3 Proteins; D004279:DNA, Viral; D001616:beta-Galactosidase