Different localization of dendritic cell reservoirs in human immunodeficiency virus-1 subtype B versus subtype E-infected lymph nodes.
The presence of p24 protein was studied in lymph nodes from human immunodeficiency virus (HIV)-positive patients affected by persistent generalized lymphadenopathy. Paraffin-embedded lymph node sections from 50 HIV-1 subtype E-infected lymph nodes from patients in Thailand and 25 HIV-1 presumably subtype B-infected lymph nodes from patients in the United States were immunostained with p24 HIV major core and capsid monoclonal antibodies using the streptavidin-biotin immunoperoxidase technique. Positivity for HIV p24 protein was detected in 20 of 22 HIV-1 subtype B infected nodes in which lymphoid follicles were present, with p24 staining demonstrating a reticular pattern within the germinal centers. Interestingly, no case from 50 clade E-infected lymph nodes containing lymphoid follicles had such a reticular pattern in the germinal centers. This difference could be explained by differential infection of subsets of dendritic cells by the two HIV-1 clades, or perhaps by different routes of initial HIV-1 transmission.
Bhoopat
L
L
Department of Pathology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand. lbhoopat@mail.med.cmu.ac.th
Rithaporn
T S
TS
Lekawanvijit
S
S
Taylor
C R
CR
Levine
A M
AM
Bhoopat
T
T
D001706:Biopsy; D016022:Case-Control Studies; D003713:Dendritic Cells / Q000821:virology*; D020566:Dendritic Cells, Follicular / Q000821:virology; D005260:Female; D018858:Germinal Center / Q000473:pathology / Q000821:virology; D016655:HIV Core Protein p24 / Q000032:analysis; D006679:HIV Seropositivity / Q000473:pathology*; D015497:HIV-1 / Q000145:classification*; D006801:Humans; D008198:Lymph Nodes / Q000473:pathology / Q000821:virology*; D008297:Male; D012189:Retrospective Studies; D013785:Thailand; D014481:United States
D016655:HIV Core Protein p24