Identification of 36-kDa flagellar phosphoproteins associated with hamster sperm motility.
In our previous paper [M. Fujinoki et al. (2001) BIOMED: Res. 22, 45-58], we reported that two types of 36-kDa protein, which were designated as 36K-A protein and 36K-B protein, obtained from hamster sperm flagella were phosphorylated at serine residues associated with the regulation of motility activation. In the present experiments, it was suggested that these two types of 36-kDa protein were phosphorylated in a cAMP-dependent manner associated with motility activation of hamster spermatozoa. Because the 36K-B protein was the most intensely phosphorylated in a cAMP-dependent manner, attempts were made to further characterize it. The 36K-B protein was assumed to be localized in the middle piece. The localization of the 36K-B protein was the same as that of the 36-kDa protein reported in our previous paper [Y. Si et al. (1999) Mol. Reprod. Dev. 52, 328-334]. In order to identify the 36K-B protein, it was analyzed by peptide mass finger printing and amino acid sequencing. The results suggested that the 36K-B protein was a pyruvate dehydrogenase E1 component beta subunit and a component of the mitochondrial sheath of the middle piece.
Fujinoki
Masakatsu
M
Department of Physiology, Dokkyo University School of Medicine, Mibu, Tochigi 321-0293, Japan. fujinoki@dokkyomed.ac.jp
Kawamura
Takeshi
T
Toda
Toshifusa
T
Ohtake
Hideki
H
Ishimoda-Takagi
Tadashi
T
Shimizu
Nobuyoshi
N
Yamaoka
Sadao
S
Okuno
Makoto
M
D000595:Amino Acid Sequence; D000818:Animals; D006224:Cricetinae; D000242:Cyclic AMP / Q000502:physiology; D005407:Flagella / Q000737:chemistry* / Q000201:enzymology / Q000502:physiology*; D008297:Male; D008647:Mesocricetus; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010449:Peptide Mapping; D010750:Phosphoproteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation; D021122:Protein Subunits / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D030481:Pyruvate Dehydrogenase (Lipoamide) / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D013081:Sperm Motility / Q000235:genetics / Q000502:physiology*; D013082:Sperm Tail / Q000737:chemistry* / Q000201:enzymology / Q000502:physiology*
D010750:Phosphoproteins; D021122:Protein Subunits; D000242:Cyclic AMP; D030481:Pyruvate Dehydrogenase (Lipoamide); C111305:pyruvate dehydrogenase E1 beta subunit