Synthesis and secretion of transferrin by isolated ciliary epithelium of rabbit.
It has been shown that the vitreous contains several intrinsic glycoproteins whose origin remains to be clarified. Isolated ciliary epithelium (CE) was assayed to verify its role in the synthesis and secretion of transferrin for the vitreous body. It was cultured in the presence of [35S]methionine and the incubation medium was processed for immunoprecipitation. Total RNA from CE was processed for RT-PCR and the amplification products were sequenced. Also, whole preparations of isolated CE were processed for immunolocalization of transferrin. From the incubation assays, a labeled peptide of about 80 kDa was immunopurified that is the expected size of transferrin. The RT-PCR and sequencing experiments detected the presence of transferrin mRNA. Both layers of the CE exhibited transferrin reactivity, following immunohistochemical processing. Taken altogether, these results indicate the CE as one of the possible sources of vitreous intrinsic transferrin.
Bertazolli-Filho
Rubens
R
Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirão Preto, USP, 14049-900 Ribeirão Preto, SP, Brazil.
Laicine
Eduardo Miguel
EM
Haddad
Antonio
A
D000818:Animals; D002924:Ciliary Body / Q000033:anatomy & histology / Q000378:metabolism*; D046508:Culture Techniques; D004848:Epithelium / Q000033:anatomy & histology / Q000378:metabolism; D007150:Immunohistochemistry; D011233:Precipitin Tests; D012333:RNA, Messenger / Q000096:biosynthesis; D011817:Rabbits; D014168:Transferrin / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger; D014168:Transferrin