Simultaneous determination of sildenafil and its active metabolite UK-103,320 in human plasma using liquid chromatography-tandem mass spectrometry.
A liquid chromatography-tandem mass spectrometric method for the simultaneous determination of sildenafil and its active N-demethylated metabolite, UK-103,320 in human plasma was developed. Sildenafil, UK-103,320 and the internal standard (DA-8159) were extracted from human plasma with dichloromethane at basic pH. A reverse-phase LC separation was performed on Luna phenylhexyl column with the mixture of acetonitrile-ammonium formate (10 mM, pH 6.0) (60:40, v/v) as mobile phase. The detection of analytes was performed using an electrospray ionization tandem mass spectrometry in the multiple reaction-monitoring mode. The lower limits of quantification for sildenafil and UK-103,320 were 2.0 ng/ml. The method showed a satisfactory sensitivity, precision, accuracy, recovery and selectivity.
Kim
John
J
Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, BC, Canada V6T 1Z3.
Ji
HyeYoung
H
Kim
Sook Jin
SJ
Lee
Hye Won
HW
Lee
Seung-Seok
SS
Kim
Dong Sung
DS
Yoo
Moohi
M
Kim
Won Bae
WB
Lee
Hye Suk
HS
D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D010879:Piperazines / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D011687:Purines; D011744:Pyrimidinones / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D000068677:Sildenafil Citrate; D013450:Sulfones
D010879:Piperazines; D011687:Purines; D011744:Pyrimidinones; D013450:Sulfones; C109995:UK 103320; D000068677:Sildenafil Citrate