title: Hormone-dependent protein patterns in integument and cuticular pigmentation in Apis mellifera during pharate adult development.
abstract: The epidermal proteins from staged Apis mellifera pupae and pharate adults and the progress of cuticular pigmentation until adult eclosion were used as parameters to study integument differentiation under hormonal treatment. Groups of bees were treated at the beginning of the pupal stage with the juvenile hormone analog pyriproxyfen (PPN) or as pharate adults with 20-hydroxyecdysone (20E). Another group was treated with both hormones applied successively at these same developmental periods. Controls were maintained without treatment. The epidermal proteins, separated by SDS-PAGE and identified by silver staining, were studied at seven intervals during the pupal and pharate adult stages. The initiation and progress of cuticular pigmentation was also monitored and compared to controls. The results showed that PPN reduced the interval of expression of some epidermal proteins, whereas 20E had an antagonistic effect, promoting a prolongation in the time of expression of the same proteins. In PPN-treated bees, cuticular pigmentation started precociously, whereas in 20E-treated individuals this developmental event was postponed. The double hormonal treatment restored the normal progress of cuticular pigmentation and, to a large extent, the temporal epidermal protein pattern. These results are discussed in relation to the 20E titer modulation and morphogenetic hormone interaction.
authors.affiliation: Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras; Departamento de Genética, Faculdade de Medicina. Universidade de São Paulo, Av Bandeirantes 3900, 14040-901, Ribeirao Preto, SP, Brazil
authors.forename: A E.
authors.initials: AE
authors.lastname: Santos
authors.forename: M M.G.
authors.initials: MM
authors.lastname: Bitondi
authors.forename: Z L.P.
authors.initials: ZL
authors.lastname: Simões