[HBsAg expression, anti-HBs induction and pathological observation in the mice inoculated with DNA vaccine against hepatitis B].
OBJECTIVE
To study if special humoral immunological response can be induced by DNA vaccine against Hepatitis B (NV-HB/s) and its mechanism.


METHODS
The Balb/C mice inoculated with NV-HB/s by intramuscular injection were detected for HBsAg(the expressed product of NV-HB/s) by ABC immunohistochemistry or ELISA, and for anti-HBs by ELISA and also performed the routine pathological examination.


RESULTS
After inoculated with NV-HB/s, HBsAg could be detected from mice muscle tissue samples of injected sites one week later and almost kept positive(75% and even more) up to 6th month, but was undetectable from all the serum samples during this period; anti-HBs could be detected from serum samples of some mice two weeks later and of all the mice detected one month later, it still kept positive at 6th month while the mice were sacrificed. Under microscope, only non-specific inflammation was found in the muscle tissues of injected sites and could completely recover within 4 weeks, no matter whether the mice were inoculated with NV-HBs, or with traditional HB vaccine derived from blood, or even with PBS as control.


CONCLUSIONS
DNA vaccine against Hepatitis B (NV-HB/s) can successfully express HBsAg in the muscle tissue of mice by intramuscular inoculation and subsequently induce anti-HBs in vitro.
Zhao
L
L
Sichuan Provincial Key Laloratory of Mdecular Biology of Infectious Diseases, First Affiliatod Hospital of Xuasi Medical University, Chengdu 610041.
Qin
S
S
Tang
H
H
D000818:Animals; D006510:Hepatitis B Antibodies / Q000096:biosynthesis*; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis*; D017325:Hepatitis B Vaccines / Q000008:administration & dosage / Q000276:immunology*; D007273:Injections, Intramuscular; D051379:Mice; D008807:Mice, Inbred BALB C; D018482:Muscle, Skeletal / Q000473:pathology*; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*
D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D019444:Vaccines, DNA