[Studies on localization of laryngeal carcinoma associated antigen by immunoelectromicroscopy].
OBJECTIVE
To study the localization of laryngeal carcinoma associated antigen (LCAA) in the carcinoma tissue.


METHODS
Ninety cases of laryngeal carcinoma and 14 cases of laryngeal precancerous lesion and 10 cases of normal laryngeal mucosa were detected with three strains of monoclonal antibodies LC9, LC11, LC12 by immunochemistry. The positive sections of laryngeal carcinoma were observed under light microscope and electromicroscopy.


RESULTS
The positive rates of LCAA were dramatically higher than that in normal epithelial and precancerous tissues (P < 0.01). The results showed that the mixed monoclonal antibody had tissue specificity. The MLC associated antigens only distributed in cell membranes and/or cytoplasm. No cell nucleus was stained.


CONCLUSIONS
The LCAA is mainly located in cell membranous structure. This study may provide morphological basis for immunoimaging diagnosis and targeting chemotherapy by application of laryngeal carcinoma McAb.
Ni
Z
Z
Department of Otorhinolaryngology, Beijing Hospital, Beijing 100730.
Yi
J
J
Xu
Y
Y
D000951:Antigens, Neoplasm / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D007822:Laryngeal Neoplasms / Q000276:immunology* / Q000473:pathology / Q000648:ultrastructure; D016253:Microscopy, Immunoelectron
D000951:Antigens, Neoplasm