Phosphate-dependent glutaminase in enterocyte mitochondria and its regulation by ammonium and other ions.
The effects of ammonium and other ions on phosphate dependent glutaminase (PDG) activity in intact rat enterocyte mitochondria were investigated. Sulphate and bicarbonate activated the enzyme in absence and presence of added phosphate. In presence of 10 mM phosphate, ammonium at concentrations <1 mM inhibited the enzyme. This inhibition was reversed by increased concentration of phosphate or sulphate. The inhibition of PDG by ammonium in presence of 10 mM phosphate was biphasic with respect to glutamine concentration, its effect being through a lowering of V(max) at glutamine concentration of </=5 mM, and increased K(m) for substrate concentration above 5 mM. The activation of the enzyme by bicarbonate was through an increase in V(max). Ammonium and bicarbonate ions may therefore be important physiological regulators of PDG. It is suggested that phosphate and other polyvalent ions may function by preventing product inhibition of the enzyme through promotion of PDG dimer formation. The dimerized enzyme may have a high affinity for glutamine and reduced sensitivity to inhibition by ammonium ions.
Masola
B
B
Department of Biochemistry, University of Zimbabwe, Mount Pleasant, Harare, Zimbabwe. masolab@yahoo.co.uk
Zvinavashe
E
E
D000818:Animals; D001639:Bicarbonates / Q000494:pharmacology; D020895:Enterocytes / Q000201:enzymology* / Q000378:metabolism; D005972:Glutaminase / Q000378:metabolism*; D005973:Glutamine / Q000494:pharmacology; D066298:In Vitro Techniques; D007477:Ions; D008297:Male; D008928:Mitochondria / Q000201:enzymology*; D010710:Phosphates / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013431:Sulfates / Q000494:pharmacology
D001639:Bicarbonates; D007477:Ions; D010710:Phosphates; D000644:Quaternary Ammonium Compounds; D013431:Sulfates; D005973:Glutamine; D005972:Glutaminase