Biochemical characterization of beta-lactamases Bla1 and Bla2 from Bacillus anthracis.
The Sterne and Ames strains of Bacillus anthracis carry chromosomal genes bla1 and bla2, which confer beta-lactam resistance when expressed in Escherichia coli. MIC measurements and steady-state kinetic analyses indicate that Bla1 possesses penicillinase activity while Bla2 possesses penicillinase, cephalosporinase, and carbapenem-hydrolyzing activities.
Materon
Isabel C
IC
Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas 77030, USA.
Queenan
Anne Marie
AM
Koehler
Theresa M
TM
Bush
Karen
K
Palzkill
Timothy
T
D000881:Anthrax / Q000382:microbiology; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001408:Bacillus anthracis / Q000201:enzymology* / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D011994:Recombinant Proteins / Q000235:genetics; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D011994:Recombinant Proteins; D047090:beta-Lactams; D001618:beta-Lactamases