title: Determination of cut-off values for leucocytes and bacteria for urine flow cytometer (UF-100) in urinary tract infections.
abstract: Leucocytes and bacteria in urine are the important indices for diagnosing urinary tract infections. Urine specimens from 260 patients were examined for leucocytes and bacteria with the UF-100 flow cytometer (Sysmex Corporation, Japan). The results obtained with the UF-100 were compared with those obtained by bacteriologic cultures of the same urine samples. Bacteriologic samples were found to be positive in 48 urine samples. The best detected cut-off value for bacteria is 3800/microL (senstivity = 73%, specificity = 78%) and 25/microL for leucocytes (sensitivity = 73%, specificity = 92%). In addition, comparison of leucocyte count by manually and with UF-100 shows good correlation (r = 0.91). A flow cytometric urinalysis analyzer operates in a time saving manner, increases throughput and decrease microscopy review rates. It also provides a reliable information to clinicians prior to bacteriologic culture results, in terms of leucocyte and bacteria count.
authors.affiliation: Department of Biochemistry, School of Medicine, Afyon Kocatepe, Turkey. tkoken@aku.eud.tr
authors.forename: Tulay
authors.initials: T
authors.lastname: Koken
authors.forename: Orhan C
authors.initials: OC
authors.lastname: Aktepe
authors.forename: Mustafa
authors.initials: M
authors.lastname: Serteser
authors.forename: Murat
authors.initials: M
authors.lastname: Samli
authors.forename: Ahmet
authors.initials: A
authors.lastname: Kahraman
authors.forename: Nurhan
authors.initials: N
authors.lastname: Dogan
mesh_terms: D001437:Bacteriuria; D015169:Colony Count, Microbial; D005434:Flow Cytometry; D006801:Humans; D007958:Leukocyte Count; D012680:Sensitivity and Specificity; D014552:Urinary Tract Infections / Q000175:diagnosis* / Q000652:urine; D014556:Urine / Q000166:cytology* / Q000382:microbiology