Preparation of vitamin K-dependent proteins, such as clotting factors II, VII, IX and X and clotting inhibitor protein C.
A review is given of preparative methods for the isolation of the vitamin K-dependent clotting factors II, VII, IX, X and clotting inhibitor protein C, all derived from human plasma. Factor II, activated factor VII and activated protein C are also obtained from recombinant animal cells. The methods for their purification are described. The problem of difference in posttranslational modifications between plasma derived and recombinant protein is discussed with regard to therapeutic proteins.
Josic
Djuro
D
Octapharma Pharmazeutika Produktions Ges.m.b.H., Oberlaaerstrasse 235, A-1100, Vienna, Austria. djuro.josic@octapharma.at
Hoffer
Lutz
L
Buchacher
Andrea
A
D000818:Animals; D001779:Blood Coagulation Factors / Q000737:chemistry* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D011486:Protein C / Q000737:chemistry* / Q000302:isolation & purification; D014812:Vitamin K / Q000494:pharmacology*
D001779:Blood Coagulation Factors; D011486:Protein C; D014812:Vitamin K