title: DNA ploidy in cervical lesions assessed by computed image analysis: relation to histopathology.
abstract: OBJECTIVE
  To assess the different patterns of DNA nuclear content in cervical intraepithelial neoplasia.
  
  
  METHODS
  DNA nuclear content patterns (DNA ploidy) were assessed in 82 paraffin embedded samples diagnosed as chronic cervicitis CIN 1, CIN 2 and CIN 3 by image analysis using the CAS200 BD System. These results were compared to the consensus diagnosis of two pathologists. The chi-square test was used to evaluate the results of classificatory variables (CIN 1, CIN 2 and CIN 3).
  
  
  RESULTS
  Aneuploidy was found in 86% of CIN 3; 59% CIN 2; 26%% CIN 1. All samples classified as chronic cervicitis were diploid.
  
  
  CONCLUSIONS
  The DNA nuclear content was found to parallel the three grades of the histological WHO classification. Aneuploidy was strongly associated with the high-grade lesions; the detection of a subset aneuploidy of low grade lesions would suggest a indicate closer follow-up of these cases.
authors.affiliation: Division of Pathology, Adolfo Lutz Institute, Avenida Dr. Arnaldo 355, Cerqueira Cezar 05025-902, São Paulo, SP, Brazil. shirata86@hotmail.Com
authors.forename: N K
authors.initials: NK
authors.lastname: Shirata
authors.forename: M C Nogueira
authors.initials: MC
authors.lastname: Zerbini
authors.forename: A
authors.initials: A
authors.lastname: Longatto Filho
authors.forename: E Sobroza
authors.initials: ES
authors.lastname: de Mello
authors.forename: V
authors.initials: V
authors.lastname: Arias
authors.forename: S
authors.initials: S
authors.lastname: Nonogaki
authors.forename: V Avancini Ferreira
authors.initials: VA
authors.lastname: Alves
mesh_terms: D000782:Aneuploidy; D002908:Chronic Disease; D004247:DNA / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D016612:Paraffin Embedding; D011003:Ploidies; D002578:Uterine Cervical Dysplasia / Q000235:genetics* / Q000473:pathology; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology; D002575:Uterine Cervicitis / Q000235:genetics* / Q000473:pathology
chemical_list: D004273:DNA, Neoplasm; D004247:DNA