title: Arsenite-induced formation of hydroxyl radical in the striatum of awake rats.
abstract: Recent studies on the mechanisms of arsenite toxicity report that some of its effects have been traced to the generation of reactive oxygen species during oxidative stress. In this study we analyze the formation of hydroxyl radicals in the brain of awake, freely moving rats, in order to obtain direct evidence of arsenic-induced oxidative stress in this tissue. We examined the time-course of hydroxyl radical formation in the striatum of both female and male rats who underwent a direct infusion during 60 min of different concentrations of arsenite in that structure through a microdialysis probe. We report here that basal levels of hydroxyl radical production in female rats are significantly higher than those in male rats (91.9+/-16.1 vs. 59.2+/-18.1 pmol/ml, P<0.001) and that the treatment with arsenite induced significant increases of hydroxyl radical formation over basal levels at 50, 100, 200 and 400 microM (95, 98, 98 and 99% increases, respectively, P<0.05 in all cases). The maximal response to 100 microM arsenite is significantly higher in female than in male rats (194.6+/-50.1 female rats and 88.1+/-11.6 pmol/ml male rats, P=0.036). These results support the participation of hydroxyl radicals in arsenic-induced disturbances in the central nervous system.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, University of San Luis, Av. V. Carranza 2405, 78210 San Luis Potosí, SLP, Mexico.
authors.forename: Erika
authors.initials: E
authors.lastname: García-Chávez
authors.forename: Abel
authors.initials: A
authors.lastname: Santamaría
authors.forename: Fernando
authors.initials: F
authors.lastname: Díaz-Barriga
authors.forename: Peter
authors.initials: P
authors.lastname: Mandeville
authors.forename: Bertha I
authors.initials: BI
authors.lastname: Juárez
authors.forename: María E
authors.initials: ME
authors.lastname: Jiménez-Capdeville
mesh_terms: D000818:Animals; D018053:Arsenites / Q000633:toxicity*; D002851:Chromatography, High Pressure Liquid; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D017665:Hydroxyl Radical / Q000378:metabolism*; D008297:Male; D017551:Microdialysis; D051381:Rats; D017208:Rats, Wistar; D017670:Sodium Compounds / Q000633:toxicity*; D014851:Wakefulness
chemical_list: D018053:Arsenites; D017670:Sodium Compounds; D017665:Hydroxyl Radical; C017947:sodium arsenite