pH-dependent photoautotrophic growth of specific photosystem II mutants lacking lumenal extrinsic polypeptides in Synechocystis PCC 6803.
The removal of either the PsbU or PsbV protein has been investigated in a cyanobacterial DeltaPsbO strain and in mutants carrying deletions or substitutions in lumen-exposed domains of CP47. These experiments have demonstrated a functional interaction between the PsbU protein and photosystem II (PSII) in the absence of the PsbO subunit. The control:DeltaPsbO:DeltaPsbU strain assembled PSII centers at pH 7.5 but did not evolve oxygen; however, photoautotrophic growth was restored at pH 10.0. In addition, several CP47 mutants, lacking extrinsic proteins, were obligate photoheterotrophs at pH 7.5 but photoautotrophic at pH 10.0, whereas other strains remained photoheterotrophs at alkaline pH.
Eaton-Rye
Julian J
JJ
Department of Biochemistry, University of Otago, P.O. Box 56, Dunedin, New Zealand. julian.eaton-rye@stonebow.otago.ac.nz
Shand
Jackie A
JA
Nicoll
William S
WS
D001426:Bacterial Proteins; D000458:Cyanobacteria / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D003574:Cytochrome c Group / Q000235:genetics / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D045342:Light-Harvesting Protein Complexes; D009154:Mutation; D010455:Peptides / Q000737:chemistry; D010788:Photosynthesis; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D045332:Photosystem II Protein Complex; D017434:Protein Structure, Tertiary; D017384:Sequence Deletion
D001426:Bacterial Proteins; D003574:Cytochrome c Group; D045342:Light-Harvesting Protein Complexes; D010455:Peptides; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; C107126:PsbU protein, Synechocystis; C070970:photosystem II, chlorophyll-binding protein, CP-47; C031732:cytochrome C-550