Sterol and triterpenoid constituents of Verbena littoralis with NGF-potentiating activity.
Two new sterols, stigmast-5-ene 3beta,4beta,7alpha,22alpha-tetraol (1) and stigmast-5-ene 3beta,7alpha,22alpha-triol (2), were isolated from the aerial parts of a Paraguayan medicinal plant, Verbena littoralis, together with the known compounds ursolic acid (3) and oleanolic acid (4). The structures of 1 and 2 were elucidated by spectroscopic analyses. Compounds 2-4 showed an enhancing activity of nerve growth factor (NGF)-mediated neurite outgrowth in PC12D cells.
Li
Yushan
Y
Department of Pharmaceutical Molecular Biology, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Sendai 980-8578, Japan.
Ishibashi
Masami
M
Satake
Masayuki
M
Chen
Xigui
X
Oshima
Yasukatsu
Y
Ohizumi
Yasushi
Y
D000818:Animals; D007564:Japan; D015394:Molecular Structure; D009414:Nerve Growth Factors / Q000187:drug effects / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects; D019906:Nuclear Magnetic Resonance, Biomolecular; D009828:Oleanolic Acid / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D016716:PC12 Cells / Q000187:drug effects; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D013237:Stereoisomerism; D013265:Stigmasterol / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects; D032412:Verbena / Q000737:chemistry*; D000097245:Ursolic Acid
D009414:Nerve Growth Factors; D009828:Oleanolic Acid; D013265:Stigmasterol; D014315:Triterpenes; D000097245:Ursolic Acid; C478286:stigmast-5-ene 3,4,7,22-tetraol; C478287:stigmast-5-ene 3,7,22-triol