Gap junctional coupling in the olfactory organ of zebrafish embryos.
Intercellular communication through gap junctions is vital for many developmental processes, including cell division and synaptogenesis. This study is the first demonstration that olfactory organ cells are functionally coupled by gap junctions. Cell coupling was examined during development in the olfactory organ using gap junction permeable dyes in live zebrafish embryos. At 1 day post-fertilization (dpf), cells of the olfactory organ were not coupled by gap junctions. At 2 and 3 dpf, olfactory organ cells passed dye from one cell to another, indicating functional coupling via gap junctions. Coupled cell cohorts included combinations of all three olfactory cell types: basal cells, support cells, and olfactory receptor cells. As the olfactory organ matured, the number of cells per coupled cell cohort increased. Gap junctional coupling corresponded with maturation of the olfactory organ and indicates that functional gap junctions may be involved in proper development of the olfactory organ.
Weber
Stacy A
SA
Department of Biomedical Sciences, College of Osteopathic Medicine, Ohio University, Athens, OH 45701, USA.
Ross
Linda S
LS
D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology; D002229:Carbenoxolone / Q000494:pharmacology; D004625:Embryo, Nonmammalian; D005314:Embryonic and Fetal Development; D005456:Fluorescent Dyes; D017629:Gap Junctions / Q000502:physiology*; D007150:Immunohistochemistry; D008239:Lysine / Q000031:analogs & derivatives* / Q000378:metabolism; D009830:Olfactory Bulb / Q000196:embryology / Q000254:growth & development / Q000502:physiology*; D012235:Rhodamines / Q000493:pharmacokinetics; D013997:Time Factors; D015027:Zebrafish / Q000196:embryology*
D000897:Anti-Ulcer Agents; D005456:Fluorescent Dyes; D012235:Rhodamines; C005358:tetramethylrhodamine; C013411:biocytin; D008239:Lysine; D002229:Carbenoxolone