[Expression of the inducible isoform of nitric oxide synthase mRNA and the role in nasal polyps].
OBJECTIVE
To explore the pathogenesis of nasal polyps. The aim of this study is to detect the expression of inducible isoform of nitric oxide synthase (iNOS) in nasal polyp tissue, and to compare these findings with that in normal nasal turbinate.


METHODS
We examined the expression of iNOS in human nasal polyps from patients undergoing nasal polypectomy (n = 8) and nasal turbinectomy(n = 6). The iNOS mRNA expression was determined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR).


RESULTS
The iNOS mRNA expression was significantly stronger in the nasal polyps (NP) than that in turbinate tissues (P < 0.01).


CONCLUSIONS
iNOS expression is upregulated in NP, which indicate that iNOS and NO may play a potential role in the formation and growth of NP.
Jiang
S
S
Department of Otolaryngology & Head and Neck Surgery, China-Japan Union Hospital, Bethune Faculty of Medicine, Jilin University, Changchun 130031, China. jiangshu1@163.net
Dong
Z
Z
Yang
Z
Z
D000328:Adult; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009297:Nasal Mucosa / Q000201:enzymology; D009298:Nasal Polyps / Q000201:enzymology*; D009569:Nitric Oxide / Q000032:analysis; D019001:Nitric Oxide Synthase / Q000096:biosynthesis* / Q000235:genetics; D052247:Nitric Oxide Synthase Type II; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014420:Turbinates / Q000201:enzymology
D012333:RNA, Messenger; D009569:Nitric Oxide; C496319:NOS2 protein, human; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II