[The comparison in double immunodiffusion and western blotting methods of anti-SS-A/B antibodies in patients with Sjögren's syndrome].
OBJECTIVE
To investigate the autoimmune responses against SS-A/B antigens by double immunodiffusion (DID) and western blotting (WB) in primary and secondary Sjögren's syndrome (SS).


METHODS
Forty-nine patients with primary SS (PSS), 28 patients with secondary SS (SSS) and control group that couldn't be diagnosed as SS were included in this study.


RESULTS
In DID analysis, Anti-SS-A antibody was detected in 69% of PSS and 86% of SSS, and anti-SS-B antibody was found in 22% of PSS and 39% of SSS. No significant difference could be demonstrated between PSS and SSS concerning anti-SS-A/B antibodies. Conversely, WB studies disclosed evidences that 18% of PSS and no SSS reacted only with the 52 kD protein, and there was significantly increased in PSS. Sera reacting with the 60 kD antigen were found in 37% of PSS, 71% of SSS, and 75% of SSS with SLE, 63% of SSS with RA. The ratio of SSS, and SSS with SLE were particularly significantly higher than PSS.


CONCLUSIONS
Our results revealed data that there are the difference of reactivity against SS-A/B antigens in WB between PSS and SSS.
Xu
Zhiquan
Z
Department of Internal Medicine and Rheumatology, Juntendo University School of Medicine.
Takeuchi
Ken
K
Matsudaira
Ran
R
Kanai
Yoshinori
Y
Tokano
Yoshiaki
Y
Takasaki
Yoshinari
Y
Hashimoto
Hiroshi
H
D000974:Antibodies, Antinuclear / Q000097:blood*; D015153:Blotting, Western; D006801:Humans; D005779:Immunodiffusion; D012859:Sjogren's Syndrome / Q000276:immunology*
D000974:Antibodies, Antinuclear; C035356:SS-A antibodies; C035357:SS-B antibodies