title: Development and validation of analytical methods for monomeric and oligomeric migrants from nylon 12 packaging materials.
abstract: Analytical methods for the determination of laurolactam--the monomer of nylon 12--as well as the cyclic dimer and trimer were established. High performance liquid chromatography using ultraviolet (HPLC-UV) and mass spectrometric detection (HPLC-MS) were both found suitable to identify and quantify monomer, cyclic dimer and trimer well below the specific migration limit (SML) of laurolactam, being 5 mg/kg of food (simulant). Gas chromatography with flame ionisation detection (GC-FID) showed to be an appropriate method for the detection of only laurolactam in aqueous and fatty food simulants. Food simulants could be analysed directly by all three methods, or after a change of solvents. For olive oil, a method for sample clean-up by size-exclusion chromatography (SEC) was established.
authors.affiliation: Fraunhofer Institute for Process Engineering and Packaging, Giggenhauser Strasse 35, 85354 Freising, Germany. stoffers@ivv.fraunhofer.de
authors.forename: N H
authors.initials: NH
authors.lastname: Stoffers
authors.forename: F
authors.initials: F
authors.lastname: Brandl
authors.forename: J P H
authors.initials: JP
authors.lastname: Linssen
authors.forename: R
authors.initials: R
authors.lastname: Franz
mesh_terms: D002849:Chromatography, Gas / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005506:Food Contamination / Q000032:analysis*; D018857:Food Packaging; D007536:Isomerism; D007769:Lactams / Q000032:analysis; D009757:Nylons; D000069463:Olive Oil; D010938:Plant Oils / Q000737:chemistry
chemical_list: D007769:Lactams; D009757:Nylons; D000069463:Olive Oil; D010938:Plant Oils; C036222:nylon 12; C474694:laurolactam