title: Intestinal polyp formation in the Apcmin mouse: effects of levels of dietary calcium and altered vitamin D homeostasis.
abstract: This study evaluated the effects of various levels of dietary calcium on polyp formation, vitamin D homeostasis, and fecal bile acids in the Apcmin mouse. Female Apcmin mice were randomized to three groups and fed a purified diet with either half or double the level of calcium in control AIN-93G. Serum 25-OH-D and fecal bile acids were measured at weeks 0 and 12 of treatment. Mice were killed for polyp scoring by two observers blinded to treatment after 12 weeks. Results show there was no difference in polyp number or tumor load with dietary calcium in any treatment group. Serum 25-OH-D was reduced and total fecal bile acids were increased in animals that received the high calcium diet. We have previously shown that vitamin D supplementation diminishes polyp load; the lack of effect of an altered calcium diet seen here may be due to a disturbance in vitamin D homeostasis.
authors.affiliation: UCLA Center for Human Nutrition, Los Angeles, California 90095, USA.
authors.forename: Sergio
authors.initials: S
authors.lastname: Huerta
authors.forename: Ronald W
authors.initials: RW
authors.lastname: Irwin
authors.forename: David
authors.initials: D
authors.lastname: Heber
authors.forename: Vay Liang W
authors.initials: VL
authors.lastname: Go
authors.forename: Farhad
authors.initials: F
authors.lastname: Moatamed
authors.forename: Sara
authors.initials: S
authors.lastname: Huerta
authors.forename: Che
authors.initials: C
authors.lastname: Ou
authors.forename: Diane M
authors.initials: DM
authors.lastname: Harris
mesh_terms: D000818:Animals; D001647:Bile Acids and Salts / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis; D002136:Calcium, Dietary / Q000008:administration & dosage*; D004195:Disease Models, Animal; D005243:Feces / Q000737:chemistry; D005260:Female; D006706:Homeostasis; D015994:Incidence; D007417:Intestinal Polyps / Q000453:epidemiology / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D011336:Probability; D011897:Random Allocation; D012016:Reference Values; D014807:Vitamin D / Q000032:analysis* / Q000378:metabolism
chemical_list: D001647:Bile Acids and Salts; D014408:Biomarkers, Tumor; D002136:Calcium, Dietary; D014807:Vitamin D