PRL-3 and PRL-1 promote cell migration, invasion, and metastasis.
We demonstrate here that Chinese hamster ovary cells stably expressing PRL-3, a M(r) 20000 prenylated protein tyrosine phosphatase, or its relative, PRL-1, exhibit enhanced motility and invasive activity. A catalytically inactive PRL-3 mutant has significantly reduced migration-promoting activity. We observe that PRL-3 is associated with diverse membrane structures involved in cell movement. Furthermore, we show that PRL-3- and -1-expressing cells, but not control cells, induce metastatic tumor formation in mice. Thus, our results deliver the first evidence for a causative role of PRL-3 and -1 in promoting cell motility, invasion activity, and metastasis.
Zeng
Qi
Q
Institute of Molecular and Cell Biology, Singapore 117609, Singapore.
Dong
Jing-Ming
JM
Guo
Ke
K
Li
Jie
J
Tan
Hui-Xian
HX
Koh
Vicki
V
Pallen
Catherine J
CJ
Manser
Edward
E
Hong
Wanjin
W
D000818:Animals; D016466:CHO Cells; D002462:Cell Membrane / Q000201:enzymology / Q000502:physiology; D002465:Cell Movement / Q000502:physiology*; D006224:Cricetinae; D005260:Female; D017874:Immediate-Early Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D009374:Neoplasms, Experimental / Q000201:enzymology* / Q000473:pathology*; D017027:Protein Tyrosine Phosphatases / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis / Q000235:genetics
D017874:Immediate-Early Proteins; D016271:Proto-Oncogene Proteins c-myc; C111534:Ptp4a3 protein, mouse; D017027:Protein Tyrosine Phosphatases; C087359:Ptp4a1 protein, mouse