14-3-3 proteins are constituents of the insoluble glycoprotein framework of the chlamydomonas cell wall.
The cell wall of the unicellular green alga Chlamydomonas reinhardtii consists predominantly of Hyp-rich glycoproteins, which also occur in the extracellular matrix of multicellular green algae and higher plants. In addition to the Hyp-rich polypeptides, the insoluble glycoprotein framework of the Chlamydomonas cell wall contains minor amounts of 14-3-3 proteins, as revealed by immunochemical studies and mass spectroscopic analysis of tryptic peptides. Polypeptides immunologically related to the 14-3-3 proteins also were found in the culture medium of Chlamydomonas. The levels of two of these 14-3-3-related polypeptides were decreased in the culture medium of the wall-deficient mutant cw-15. These findings indicate that 14-3-3 proteins are involved in the cross-linking of Hyp-rich glycoproteins in the Chlamydomonas cell wall.
Voigt
Jürgen
J
Physiologisch-Chemisches Institut, Eberhard-Karls-Universität Tübingen, Hoppe-Seyler-Strasse 4, D-72076 Tübingen, Germany. juergen.voigt@medizin.uni-leipzig.de
Frank
Ronald
R
D048948:14-3-3 Proteins; D020418:Algal Proteins / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D002473:Cell Wall / Q000276:immunology / Q000378:metabolism*; D016825:Chlamydomonas reinhardtii / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D018604:Epitope Mapping / Q000379:methods; D006023:Glycoproteins / Q000276:immunology / Q000378:metabolism*; D006031:Glycosylation; D015151:Immunoblotting; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D020539:Sequence Analysis, Protein; D014446:Tyrosine 3-Monooxygenase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D048948:14-3-3 Proteins; D020418:Algal Proteins; D006023:Glycoproteins; D020033:Protein Isoforms; D014446:Tyrosine 3-Monooxygenase