A rapid method for the construction of oligonucleotide arrays.
A simple method has been devised to construct oligonucleotide array on a variety of surfaces using commonly available reagents and chemistry with good efficiency and accuracy. The method involves the generation of hydroxyl functionalities on glass, polypropylene, polyethylene, and commonly used surfaces for construction of oligonucleotide arrays followed by their activation with trifluoroethanesulfonyl chloride (tresyl chloride). The activated surface in the subsequent reaction is used to covalently immobilize oligonucleotides in regioselective fashion to create an oligonucleotide array. The surface bound tresyl sulfonate esters allow the immobilization of oligonucleotides specifically via their 3'- or 5'-end having mercaptohexyl- or aminohexyl functionalities. The constructed oligonucleotide arrays were successfully used to analyze oligonucleotides by hybridization technique.
Kumar
P
P
Nucleic Acids Research Laboratory, Institute of Genomics and Integrative Biology (formerly Centre for Biochemical Technology), Mall Road, Delhi University Campus, Delhi-110 007, India.
Gupta
K C
KC
D017665:Hydroxyl Radical / Q000737:chemistry; D007700:Kinetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes / Q000737:chemistry; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000302:isolation & purification; D010777:Photochemistry / Q000379:methods; D011108:Polymers / Q000737:chemistry
D015345:Oligonucleotide Probes; D009841:Oligonucleotides; D011108:Polymers; D017665:Hydroxyl Radical