Effect of age on body distribution of Tityustoxin from Tityus serrulatus scorpion venom in rats.
Previous research from our Laboratory has shown a greater susceptibility of young animals, when compared to adults, to envenomation by tityustoxin (TsTX), one of the main toxins from Tityus serrulatus scorpion venom. Our hypothesis is that a differential body distribution of TsTX among adult and young animals could account for the worse prognosis of scorpion envenomation in infants. Thus, TsTX labeled with technetium-99m was injected (6 microg, subcutaneous) in adult (150-160 day-old) and young (21-22 day-old) male rats. Groups of animals were sacrificed at different times after TsTX injection (0.08, 1.0, 3.0, 6.0, 12.0 and 24.0 hours) under Urethane anesthesia (140 mg/100 g, i.p.). The brain, heart, lungs, liver, kidneys, spleen and thyroid were excised and blood collected. Young rats presented a shorter latency toxin concentration peak in all studied organs except for the liver and the kidney, when compared to adults. The ratio between the area under the curve of the toxin concentration in each organ and that in blood (Kp) indicates higher accumulation in the organs of young animals mainly for brain, liver and heart. These observations suggest a faster toxin distribution in the organs of young rats. The higher uptake of TsTX in the brain is suggestive of a greater permeability for the toxin along the blood-brain barrier of young rats. In conclusion, the higher uptake in heart, together with data from the brain, may help to elucidate the clinical manifestations frequently observed in children under scorpion envenomation.
Nunan
Elzíria A
EA
Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Olegário Maciel, 2360, Lourdes, CEP 30180-112, Belo Horizonte, Minas Gerais, Brazil.
Moraes
Márcio F D
MF
Cardoso
Valbert N
VN
Moraes-Santos
Tasso
T
D000375:Aging / Q000378:metabolism*; D000818:Animals; D019540:Area Under Curve; D002850:Chromatography, Gel; D007279:Injections, Subcutaneous; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012604:Scorpion Venoms / Q000302:isolation & purification / Q000493:pharmacokinetics*; D012605:Scorpions; D013053:Spectrophotometry; D013667:Technetium; D014018:Tissue Distribution
D012604:Scorpion Venoms; C003609:tityustoxin; D013667:Technetium