Effect of selenium-enriched broccoli diet on differential gene expression in min mouse liver(1,2).
Multiple intestinal neoplasia (Min) mice are a good model for investigating the effects of dietary alterations in a genetic model for intestinal cancer. Previous studies have shown that selenium-enriched broccoli effectively reduces colon cancer susceptibility. Although colon cancer cells mainly metastasize to the liver, little is known about the effects of selenium-enriched broccoli on gene expression in mouse liver. To better understand the protective role for selenium-enriched broccoli in tumorigenesis, a gene profile of the mouse liver was analyzed. Mice were fed either 0.11 mg selenium/kg control diet or 2.1 mg selenium/kg selenobroccoli diets for 10 weeks. Use of mouse pathway finder-1 GEArrays revealed that selenium-enriched broccoli moderately increased ikBalphakappaB, hsp86, gadd45 gene transcripts. In addition, analysis of the binding of liver nuclear proteins to (32)P-labeled probes demonstrated that selenium-enriched broccoli enhanced the binding of transcription factor p53, NFkappaB, AP-1 to their cis-acting elements. Collectively, these results suggest for the first time that selenium-enriched broccoli activates certain pro-apoptotic genes linked to p53, NFkappaB and stress signal pathways in response to "danger signals" such as tumorigenesis.
Zeng
Huawei
H
United States Department of Agriculture, Grand Forks Human Nutrition Research Center, Grand Forks, North Dakota 58202-9034, USA. hzeng@gfhnrc.ars.usda.gov
Davis
Cindy D
CD
Finley
John W
JW
D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D001937:Brassica / Q000737:chemistry*; D004032:Diet; D024202:Electrophoretic Mobility Shift Assay; D015870:Gene Expression / Q000187:drug effects*; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics; D020672:I-kappa B Proteins / Q000235:genetics; D007414:Intestinal Neoplasms / Q000235:genetics; D047908:Intracellular Signaling Peptides and Proteins; D008099:Liver / Q000737:chemistry*; D008113:Liver Neoplasms / Q000556:secondary; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000235:genetics; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D012643:Selenium / Q000008:administration & dosage*; D000097642:GADD45 Proteins
D018841:HSP90 Heat-Shock Proteins; D020672:I-kappa B Proteins; D047908:Intracellular Signaling Peptides and Proteins; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B; D011506:Proteins; D012333:RNA, Messenger; D012643:Selenium; D000097642:GADD45 Proteins; C107835:Hspca protein, mouse; C000606204:NFKBIA protein, human; C000606205:Nfkbia protein, mouse