Oxidative parameters and mortality in sepsis induced by cecal ligation and perforation.
OBJECTIVE
This study assessed parameters of free radical damage to biomolecules, mitochondrial superoxide production, superoxide dismutase, and catalase activities and their relationship to sepsis mortality.


METHODS
Prospective animal study in a university laboratory for experimental.


METHODS
140 male Wistar rats.


METHODS
The animals were randomly divided into three groups: sham-operated (n=20), cecal ligation and perforation resuscitated with normal saline (n=40), and cecal ligation and perforation with normal saline plus antibiotics (n=40).


RESULTS
Blood samples were collected from all animals 3, 12, and 24 h after CLP through a jugular catheter inserted before CLP. Rats were evaluated during 5 days after the intervention. Nonsurvivor animals were grouped according to the duration between sepsis induction and death, and oxidative parameters were compared to survivors and sham-operated. Lipid peroxidation, protein carbonyls, and superoxide dismutase were significantly increased in nonsurvivor septic rats and were predictive of mortality. We demonstrated that there is a different modulation of superoxide dismutase and catalase in nonsurvivors during the course of septic response. There was a marked increase in superoxide dismutase activity without a proportional increase in catalase activity in nonsurvivors.


CONCLUSIONS
This is the first report of plasma superoxide dismutase as an earlier marker of mortality. Ours results might help to clarify an important aspect of oxidative response to sepsis, i.e., an increase in superoxide dismutase activity without a proportional increase in catalase activity
Ritter
Cristiane
C
Departamento de Bioquímica, Centro de Estudos em Estresse Oxidativo, UFRGS, Ramiro Barcelos 2600 anexo, Porto Alegre, 90035-003 Brazil.
Andrades
Michael
M
Frota Júnior
Mário Luis C
ML
Bonatto
Fernanda
F
Pinho
Ricardo A
RA
Polydoro
Manuela
M
Klamt
Fábio
F
Pinheiro
Cleovaldo T S
CT
Menna-Barreto
Sérgio S
SS
Moreira
José Cláudio F
JC
Dal-Pizzol
Felipe
F
D000818:Animals; D002429:Cecal Diseases / Q000150:complications; D002432:Cecum; D007416:Intestinal Perforation / Q000150:complications; D008026:Ligation; D008297:Male; D018384:Oxidative Stress; D011446:Prospective Studies; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000209:etiology / Q000378:metabolism* / Q000401:mortality*