A new imprinted cluster on the human chromosome 7q21-q31, identified by human-mouse monochromosomal hybrids.
We have previously established a series of human monochromosomal hybrids containing a single human chromosome of defined parental origin as an in vitro resource for the investigation of human imprinted loci. Using the hybrids with a paternal or maternal human chromosome 7, we determined the allelic expression profiles of 76 ESTs mapped to the human chromosome 7q21-q31. Seven genes/transcripts, including PEG10 which has previously been reported to be imprinted, showed parent-of-origin-specific expression in monochromosomal hybrids. One of the 6 candidate genes/transcripts, i.e., DLX5 was confirmed to be imprinted in normal human lymphoblasts and brain tissues by a polymorphic analysis. Thus, an imprinted domain has been newly defined in the region of human chromosome 7q21-q31 using human-mouse monochromosomal hybrids.
Okita
Chiga
C
Division of Molecular and Cell Genetics, Department of Molecular and Cellular Biology, School of Life Sciences, Faculty of Medicine, Tottori University, Tottori 683-8503, Japan.
Meguro
Makiko
M
Hoshiya
Hidetoshi
H
Haruta
Masayuki
M
Sakamoto
Yu-ki
YK
Oshimura
Mitsuo
M
D000818:Animals; D051017:Apoptosis Regulatory Proteins; D001921:Brain / Q000378:metabolism; D002678:Chimera; D002897:Chromosomes, Human, Pair 7 / Q000235:genetics*; D004268:DNA-Binding Proteins; D020224:Expressed Sequence Tags; D018392:Genomic Imprinting / Q000235:genetics*; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D051379:Mice / Q000235:genetics; D005810:Multigene Family / Q000235:genetics*; D011506:Proteins / Q000235:genetics; D016601:RNA-Binding Proteins; D014157:Transcription Factors
D051017:Apoptosis Regulatory Proteins; C100596:DLX5 protein, human; D004268:DNA-Binding Proteins; C501120:Dlx5 protein, mouse; D018398:Homeodomain Proteins; C432965:PEG10 protein, human; D011506:Proteins; D016601:RNA-Binding Proteins; D014157:Transcription Factors