Isolation and PCR detection of rickettsiae from clinical and rodent samples in Malaysia.
Isolation of rickettsiae from patients' blood samples and organ samples of wild rodents from areas with high seroprevalence of rickettsial infections was attempted using cell culture assay and animal passages. L929 mouse fibroblast cells grown in 24 well tissue culture plate were inoculated with buffy coat of febrile patients and examined for the growth of rickettsiae by Giemsa, Gimenez staining and direct immunofluorescence assay. No rickettsiae were isolated from 48 patients' blood samples. No symptomatic infections were noted in mice or guinea pigs infected with 50 organ samples of wild rodents. There was no rickettsial DNA amplified from these samples using various PCR detection systems for Orientia tsutsugamushi, typhus and spotted fever group rickettsiae.
Tay
S T
ST
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Malaysia. tayst@ummc.edu.my
Rohani
M Y
MY
Ho
T M
TM
Devi
S
S
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001399:Azure Stains; D018929:Cell Culture Techniques; D002648:Child; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D006168:Guinea Pigs; D006801:Humans; D007124:Immunoenzyme Techniques; D008296:Malaysia / Q000453:epidemiology; D008297:Male; D051379:Mice; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D011159:Population Surveillance; D012281:Rickettsia / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D012282:Rickettsia Infections / Q000097:blood / Q000453:epidemiology / Q000276:immunology / Q000382:microbiology* / Q000662:veterinary*; D012376:Rodent Diseases / Q000097:blood / Q000453:epidemiology / Q000276:immunology / Q000382:microbiology*; D012377:Rodentia; D016036:Seroepidemiologic Studies
D000907:Antibodies, Bacterial; D001399:Azure Stains; D004269:DNA, Bacterial