Serum relaxin concentrations and reproduction in male bonnethead sharks, Sphyrna tiburo.
Relaxin is a 6-kd polypeptide hormone that is responsible for regulating several reproductive processes in female vertebrates, but its role in male reproduction remains unclear. To aid in clarifying this role, the objective of the present study was to investigate changes in endogenous relaxin levels associated with reproductive events in male elasmobranchs, which represent one of only three vertebrate groups known to possess this hormone. Serum relaxin concentrations were measured in 27 immature and 66 mature male bonnethead sharks (Sphyrna tiburo), a species with a well-characterized, seasonal reproductive cycle. Temporal changes in serum relaxin concentrations of immature male S. tiburo were not observed. In contrast, a temporal cycle in serum relaxin concentrations of mature male S. tiburo was observed in individuals from two sampling locations. Significant increases (P<0.05) in serum relaxin concentrations of mature male S. tiburo from both collection sites occurred during late spermatogenesis and the mating period, two critical stages of the reproductive cycle. The results from this study suggest that relaxin may play an important role in regulating semen quality, or other aspects of reproduction in male sharks. This is the first study to demonstrate a temporal pattern in endogenous serum Rlx concentrations associated with reproductive events in feral vertebrates. As such, it strengthens earlier hypotheses that suggested a role for this hormone in regulating male vertebrate fertility and copulatory success.
Gelsleichter
James
J
Center for Shark Research, Mote Marine Laboratory, 1600 Ken Thompson Parkway, Sarasota, FL 34236, USA. jimg@mote.org
Steinetz
Bernard G
BG
Manire
Charles A
CA
Ange
Cristal
C
D000818:Animals; D008297:Male; D009994:Osmolar Concentration; D012065:Relaxin / Q000097:blood*; D012098:Reproduction / Q000502:physiology*; D012621:Seasons; D012727:Sex Characteristics; D012726:Sexual Behavior, Animal / Q000502:physiology; D012741:Sexual Maturation; D012754:Sharks / Q000097:blood / Q000502:physiology*; D013091:Spermatogenesis / Q000502:physiology
D012065:Relaxin