[Suppression of nonsense and frameshift mutations obtained by different methods for inactivating the translation termination factor eRF3 in yeast Saccharomyces cerevisiae].
Mutations in genes of omnipotent nonsense suppressors SUP35 and SUP45 in yeast Saccharomyces cerevisiae encoding translation termination factors eRF3 and eRF1, respectively, and prionization of the eRF3 protein may lead to the suppression of some frameshift mutations (CPC mutations). Partial inactivation of the translation termination factor eRF3 was studied in strains with unstable genetically modified prions and also in transgenic yeast S. cerevisiae strains with the substitution of the indigenous SUP35 gene for its homolog from Pichia methanolica or for a recombinant S. cerevisiae SUP35 gene. It was shown that this partial inactivation leads not only to nonsense suppression, but also to suppression of the frameshift lys2-90 mutation. Possible reasons for the correlation between nonsense suppression and suppression of the CPC lys2-90 mutation and mechanisms responsible for the suppression of CPC mutations during inactivation of translation termination factors are discussed.
Zadorskiĭ
S P
SP
Department of Genetics and Breeding, St. Petersburg State University, St. Petersburg, 199034 Russia.
Borkhsenius
A S
AS
Sopova
Iu V
IuV
Startsev
V A
VA
Inge-Vechtomov
S G
SG
D018389:Codon, Nonsense; D016368:Frameshift Mutation; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D005800:Genes, Fungal; D016153:Genes, Suppressor; D009154:Mutation; D030781:Organisms, Genetically Modified; D010454:Peptide Termination Factors / Q000235:genetics / Q000378:metabolism*; D010843:Pichia / Q000235:genetics; D011328:Prions / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics
D018389:Codon, Nonsense; D005656:Fungal Proteins; D010454:Peptide Termination Factors; D011328:Prions; D011994:Recombinant Proteins; C080775:SUP45 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C087971:peptide-chain-release factor 3