In vitro effects of new generation fungal derived nitric oxide donors on rabbit basilar artery.
The fungal derived nitric oxide donors, (E)-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide (FK409) and N-[(E)-4-ethyl-3-[(Z)-hydroxyimino]-5-nitro-3-hexen-1-yl]-3-pyridinecarboxamide (FR144420), were evaluated for the treatment and prevention of cerebral vasospasm induced by subarachnoid hemorrhage (SAH) by an in vitro study using rabbit basilar artery. The tension-relaxation of a 3 mm-long artery segment was carried out in a micro-tissue organ bath with a real-time recorder to record the tension-relaxation curve. Steady contraction of the specimens was induced by KCI (n = 12) and oxyhemoglobin (oxyHb) (n = 12). Sodium nitroprusside was used for comparison. Each of the agents was added in ascending concentration. Relaxation caused by FK409 and FR144420 was significantly greater (p < 0.05) than that by sodium nitroprusside. Relaxation effects of FK409 and FR144420 on the KCl-induced steady contraction were better than those on the oxyHb-induced contraction. FK409 and FR144420 have potential uses for the treatment and prevention of SAH-induced cerebral vasospasm.
Islam
Md Shafiqul
MS
Department of Neurosurgery, Hirosaki University School of Medicine, Hirosaki, Aomori, Japan.
Ohkuma
Hiroki
H
Kimura
Masahide
M
Suzuki
Shigeharu
S
D000818:Animals; D001488:Basilar Artery / Q000187:drug effects*; D005260:Female; D005658:Fungi / Q000737:chemistry*; D008297:Male; D009539:Nicotinic Acids / Q000494:pharmacology*; D020030:Nitric Oxide Donors / Q000302:isolation & purification* / Q000494:pharmacology*; D009574:Nitro Compounds / Q000494:pharmacology*; D011817:Rabbits; D014661:Vasoconstriction / Q000187:drug effects; D014665:Vasodilator Agents / Q000494:pharmacology*
C094368:FR 144420; D009539:Nicotinic Acids; D020030:Nitric Oxide Donors; D009574:Nitro Compounds; D014665:Vasodilator Agents; C061872:FK 409