In vitro effects of a chlorhexidine controlled delivery system.
The aim of this work was study the effect of the chlorhexidine : hydroxypropyl-beta-cyclodextrin (CLX : HP-beta-CD) inclusion compound (IC) on in vitro slabs of bovine dentine. The substantivity, antimicrobial activity, and morphological effect of this inclusion compound were evaluated. Cyclodextrin improves the physical-chemical and pharmacological properties of drugs. Fragments of bovine dentine were immersed into either IC serial solutions at 0.24%, 0.12%, 0.06%, 0.03%, 0.015%, and 0.008% or controls water and free chlorhexidine. The desorption kinetics showed that CLX : HP-beta-CD compound release CLX for 6 days in a rate flow near to zero-order profile in comparison to plain CLX. Antimicrobial activity tests showed that CLX : HP-beta-CD inhibited A. actinomycetemcomitans and S. mutans significantly. The morphological effect studied by scanning electron microscopy (SEM) showed that CLX : HP-beta-CD did not cause morphological changes to the slab's surface. It is concluded that the chlorhexidine : hydroxypropyl-beta-CD inclusion compound creates an effective controlled release system with biological activity and that it may act as a good prevention and control agent of caries and periodontal disease in vivo.
Franco
Carolina Ferreira
CF
School of Dentistry, Cirurgia e Patologia, Faculdade de Odontologia, UFMG, Belo Horizonte, MG, Brazil.
Pataro
André Luiz
AL
E Souza
Leandra Cristina Ribeiro
LC
Santos
Vagner Rodrigues
VR
Cortés
María Esperanza
ME
Sinisterra
Rubén Dario
RD
D000818:Animals; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage* / Q000494:pharmacology; D002417:Cattle; D002710:Chlorhexidine / Q000008:administration & dosage* / Q000494:pharmacology; D003505:Cyclodextrins / Q000008:administration & dosage* / Q000494:pharmacology; D003804:Dentin / Q000187:drug effects* / Q000382:microbiology / Q000648:ultrastructure; D016503:Drug Delivery Systems; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D018709:Statistics, Nonparametric
D000891:Anti-Infective Agents, Local; D003505:Cyclodextrins; D002710:Chlorhexidine