title: Osteoblast precursor cell attachment on heat-treated calcium phosphate coatings.
abstract: The influence of properties of calcium phosphate (CaP) coatings on bone cell activity and bone-implant osseointegration is not well-established. This study investigated the effects of characterized CaP coatings of various heat treatments on osteoblast response. It was hypothesized that heat treatments of CaP coatings alter the initial osteoblast attachment. The 400 degrees C heat-treated coatings were observed to exhibit poor crystallinity and significantly greater phosphate or apatite species compared with as-sputtered and 600 degrees C heat-treated coatings. Similarly, human embryonic palatal mesenchyme (HEPM) cells, an osteoblast precursor cell line, seeded on 400 degrees C heat-treated coatings, exhibited significantly greater cell attachment compared with Ti surfaces, as-sputtered coatings, and 600 degrees C heat-treated coatings. The HEPM cells on Ti surfaces and heat-treated coatings were observed to attach through filopodia, and underwent cell division, whereas the cells on as-sputtered coatings displayed fewer filopodia extensions and cell damage. Analysis of the data suggested that heat treatment of CaP coatings affects cell attachment.
authors.affiliation: University of Texas Health Science Center at San Antonio, Department of Restorative Dentistry, Division of Biomaterials, MSC 7890, 7703 Floyd Curl Drive, San Antonio, TX 78229-3900,USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Yang
authors.forename: J D
authors.initials: JD
authors.lastname: Bumgardner
authors.forename: R
authors.initials: R
authors.lastname: Cavin
authors.forename: D L
authors.initials: DL
authors.lastname: Carnes
authors.forename: J L
authors.initials: JL
authors.lastname: Ong
mesh_terms: D001031:Apatites / Q000737:chemistry; D002130:Calcium Phosphates / Q000737:chemistry*; D002448:Cell Adhesion / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003461:Crystallography; D003764:Dental Materials / Q000737:chemistry; D004577:Electron Probe Microanalysis; D006358:Hot Temperature; D006801:Humans; D008422:Materials Testing; D008648:Mesoderm / Q000166:cytology*; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000502:physiology*; D011554:Pseudopodia / Q000648:ultrastructure; D013234:Stem Cells / Q000502:physiology*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D001031:Apatites; D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; D003764:Dental Materials; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; C485838:calcium phosphate, monobasic, anhydrous; C020243:calcium phosphate; D014025:Titanium; C485829:calcium phosphate, dibasic, anhydrous