title: [Construction and screening of suppression subtractive hybridization library of renal cell carcinoma].
abstract: OBJECTIVE
  To construct and screen the suppression subtractive hybridization (SSH) library of human renal cell carcinoma (RCC).
  
  
  METHODS
  Poly A(+) RNA was isolated from RCC lines 786-O (tester) and renal cell (RC) lines HK-2 (driver), respectively. SSH procedure was performed according to the protocol of the PCR-Select cDNA Subtraction Kit (Clontech), and PCR products were cloned into pT-Adv vector and transformed E. coli TOP10F'. All positive clones picked out were digested and some of which were sequenced.
  
  
  RESULTS
  The SSH library contained 362 clones with SSH cDNA fragments distributed mainly from 0.3 to 0.9 kb. Among 50 clones sequenced randomly, 2 represented unknown genes and the other 48 derived from 36 known genes.
  
  
  CONCLUSIONS
  The quality of the SSH library of human RCC is reliable and its construction is the basis for further screening differentially expressed genes of RCC.
authors.affiliation: Institute of Urology, First Hospital, Peking University, Beijing 100034, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jun-kui
authors.initials: JK
authors.lastname: Ai
authors.forename: Zhi-wen
authors.initials: ZW
authors.lastname: Zhang
authors.forename: Xiong-jun
authors.initials: XJ
authors.lastname: Ye
authors.forename: Hong-jian
authors.initials: HJ
authors.lastname: Zhu
authors.forename: Dian-qi
authors.initials: DQ
authors.lastname: Xin
authors.forename: Li-li
authors.initials: LL
authors.lastname: Liang
authors.forename: Yan-qun
authors.initials: YQ
authors.lastname: Na
authors.forename: Ying-lu
authors.initials: YL
authors.lastname: Guo
mesh_terms: D018262:Adenocarcinoma, Clear Cell / Q000235:genetics*; D045744:Cell Line, Tumor; D015723:Gene Library; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics*; D009693:Nucleic Acid Hybridization / Q000379:methods*