title: Characterization of the S-RNase promoters from sweet cherry (Prunus avium L.).
abstract: Genomic DNA fragments containing the S(3)-, S(4)-, and S(6)-RNase genes were isolated from the sweet cherry (Prunus avium L.) and sequenced. Comparison of the 5'-flanking sequences of these three S-RNases indicated that a highly conserved region (designated CR) existed just upstream from the putative TATA boxes. We postulate that CR contains cis-regulatory element(s) involved in pistil expression. To examine the activity of the isolated S-RNase promoters of sweet cherry in the pistil, we transiently introduced approximately 650-bp fragments of the S(4)- and S(6)-RNase promoters fused to beta-glucuronidase (GUS) gene into the pistil of the petunia using a particle bombardment technique. Histochemical analysis showed that the 5'-flanking region of each S-RNase was active in the pistil. This suggests that cis-regulatory element(s) for pistil-specific expression may exist(s) within the 650-bp region upstream from the TATA box in the sweet cherry S-RNase promoter.
authors.affiliation: JST, Regional Joint Research Project of Yamagata Prefecture, Sagae, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Ishizaka
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nakano
authors.forename: Takashi
authors.initials: T
authors.lastname: Suzuki
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Kitashiba
mesh_terms: D024506:5' Flanking Region; D001483:Base Sequence; D019470:Biolistics; D015723:Gene Library; D017930:Genes, Reporter; D008969:Molecular Sequence Data; D032306:Petunia / Q000235:genetics; D011401:Promoter Regions, Genetic; D027861:Prunus / Q000235:genetics*; D012260:Ribonucleases / Q000235:genetics*; D016415:Sequence Alignment; D017385:Sequence Homology
chemical_list: D012260:Ribonucleases