Apolipophorin-III affects the activity of the haemocytes of Galleria mellonella larvae.
Apolipophorin-III (apoLp-III) impaired the adhesion of plasmatocytes and a granular cell-subpopulation of larval Galleria mellonella to glass slides. The protein bound to haemocytes, limited the responses of the plasmatocytes to Bacillus subtilis and increased the percentage of a subgroup of granular cells with adhering bacteria. The total number of bacteria adhering to all the haemocytes on the slides declined. Injections of apoLp-III slowed bacterial removal from the haemolymph without affecting total haemocyte counts and impaired haemocyte attachment to glass slides. Purified apoLp-III bound to B. subtilis. ApoLp-III in serum bound to bacteria within 5 min, peaked at 15 min and was either shed or dissociated by 60 min. ApoLp-III bound to B. subtilis lowered the adhesion of the bacteria to the haemocytes and slowed the removal of the bacteria from the haemolymph.
Zakarian
Robert J.
RJ
Department of Natural Resource Sciences, McGill University, 21, 111 Lakeshore Road, Ste. Anne de Bellevue, H9X 3V9, Québec, Canada
Dunphy
Gary B.
GB
Albert
Paul J.
PJ
Rau
Manfred E.
ME