title: [Expression of human papillomavirus DNA in cholesteatoma of the middle ear].
abstract: OBJECTIVE
  To reveal the role of HPV in the occurrence and development of cholesteatoma of the middle ear.
  
  
  METHODS
  PCR and nuclear acid hybridization were applied in screening 44 cases of middle ear cholesteatoma for detection of HPV DNA, and a comparison analysis with the pathological results of 35 cases of middle ear cholesteatoma was made.
  
  
  RESULTS
  Pathologically, the characteristics of HPV induced-lesions was found in 12 of 35 specimens (34.3%). 44 cases of middle ear cholesteatoma were examined with consensus primers PCR and digoxigenin labeled HPV general probes. HPV DNA positive rates were 29.5% (13/44) and 25.0% (11/44) respectively. HPV DNA positive rate was 58.3% (7/12) and 13.0% (3/23) respectively in 12 cases with pathological characteristics of HPV induced-lesions and in 23 cases without those characteristics. There was significant difference(chi 2 = 7.926, P < 0.005).
  
  
  CONCLUSIONS
  HPV infection can arouse the cleavage and proliferation of cholesteatoma epithelium and may play certain role in the occurrence and development of middle ear cholesteatoma. Aggressive, papillomatous growth and koilocytes can be served as the initial proof of HPV infection in cholesteatoma of the middle ear.
authors.affiliation: Department of Otorhinolaryngology, First Hospital Of Xi'an Jiaotong University, Xi'an 710061, China. yanxiab@163.net
authors.forename: Y
authors.initials: Y
authors.lastname: Bai
authors.forename: L
authors.initials: L
authors.lastname: Yan
authors.forename: S
authors.initials: S
authors.lastname: Li
authors.forename: Q
authors.initials: Q
authors.lastname: Bai
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D018424:Cholesteatoma, Middle Ear / Q000473:pathology / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis*; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections
chemical_list: D004279:DNA, Viral