Role of adhesion molecules in mobilization of hematopoietic cells.
OBJECTIVE
To study the changes of adhesion molecules' expressions during the recombinant human granulocyte-colony-stimulating factor (rhG-CSF) mobilization in peripheral blood stem cell transplantation (PBSCT), and to confirm the influence of rhG-CSF on hematopoietic stem cells, which are proposed to guide mobilization in PBSCT.


METHODS
Mice were injected subcutaneously with diluted rhG-CSF or normal saline for 7 days. The blood Sca-1(+) stem cell count and bone marrow (BM) nucleated cell count were enumerated. The expressions of CD49d and CD44 and the adhesive ability of mononuclear cells to bone marrow matrix (fibronectin) were examined by flow cytometry and (51)Cr adhesive assay, respectively.


RESULTS
The mobilizing effect of rhG-CSF on mice was the same as on humans. The number of Sca-1(+) cells in peripheral blood reached the peak on the seventh day, the BM nucleated cell count was reduced, and the expressions of CD49d and the cells' adhesive ability in BM and PB declined.


CONCLUSIONS
rhG-CSF can reduce some cell adhesion molecules' expressions and the adhesive ability of hematopoietic stem cells to BM matrix, therefore mobilizing hematopoietic stem cells (HSC) from the BM to the peripheral blood.
Chen
Tong
T
Department of Hematology, Shanghai Huashan Hospital of Fudan University, Shanghai 200040, China.
Xie
Yi
Y
D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects / Q000502:physiology; D005260:Female; D005353:Fibronectins / Q000502:physiology; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D019650:Hematopoietic Stem Cell Mobilization; D018380:Hematopoietic Stem Cell Transplantation; D018960:Hyaluronan Receptors / Q000032:analysis / Q000502:physiology*; D039441:Integrin alpha4 / Q000032:analysis / Q000502:physiology*; D007963:Leukocytes, Mononuclear / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins
D005353:Fibronectins; D018960:Hyaluronan Receptors; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D039441:Integrin alpha4