Characterization of thioredoxin y, a new type of thioredoxin identified in the genome of Chlamydomonas reinhardtii.
The sequencing of the Arabidopsis genome revealed a multiplicity of thioredoxins (TRX), ubiquitous protein disulfide oxido-reductases. We have analyzed the TRX family in the genome of the unicellular green alga Chlamydomonas reinhardtii and identified eight different thioredoxins for which we have cloned and sequenced the corresponding cDNAs. One of these TRXs represents a new type that we named TRX y. This most probably chloroplastic TRX is highly conserved in photosynthetic organisms. The biochemical characterization of the recombinant protein shows that it exhibits a thermal stability profile and specificity toward target enzymes completely different from those of TRXs characterized so far.
Lemaire
S D
SD
Institut de Biotechnologie des Plantes, UMR 8618 CNRS, Université Paris-Sud, Bâtiment 630, 91405 Cedex, Orsay, France. lemaire@ibp.u-psud.fr
Collin
V
V
Keryer
E
E
Quesada
A
A
Miginiac-Maslow
M
M
D020418:Algal Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D016825:Chlamydomonas reinhardtii / Q000145:classification / Q000235:genetics*; D006597:Fructose-Bisphosphatase / Q000378:metabolism; D016678:Genome; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008291:Malate Dehydrogenase / Q000378:metabolism; D050538:Malate Dehydrogenase (NADP+); D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D013879:Thioredoxins / Q000145:classification / Q000235:genetics* / Q000378:metabolism*
D020418:Algal Proteins; D013879:Thioredoxins; D008291:Malate Dehydrogenase; D050538:Malate Dehydrogenase (NADP+); D006597:Fructose-Bisphosphatase