Large-scale ion-exchange column chromatography of proteins. Comparison of different formats.
This review describes the performance of various column designs available to process-scale users of low-pressure chromatography for protein purification. By carrying out a range of ion-exchange separations using Whatman microgranular ion-exchange celluloses we are able to compare and contrast the practical performance issues associated with several designs of axial and radial flow columns.
Levison
Peter R
PR
Whatman International Ltd., Springfield Mill, James Whatman Way, Kent ME14 2LE, Maidstone, UK. plevison@whatman.co.uk
D000837:Anion Exchange Resins; D002852:Chromatography, Ion Exchange / Q000379:methods*; D011506:Proteins / Q000302:isolation & purification*
D000837:Anion Exchange Resins; D011506:Proteins