title: Direct determination of benzamides in serum by column-switching high-performance liquid chromatography.
abstract: A column-switching high-performance liquid chromatographic method with fluorescence detection was developed for the simultaneous determination of four benzamide-type anti-psychotic drugs: sulpiride, tiapride, sultopride and metoclopramide in human serum. In this method, a TSKgel Super-ODS column was used as an analytical column, and a TSKgel G 2000SW was prepared as a pretreatment column. Under the optimized analytical conditions, four benzamide-type anti-psychotic drugs were eluted within 18 min. The detection limits (S/N = 3) for sulpiride, tiapride, sultopride and metoclopramide are 1 ng/ml, 4 ng/ml, 2 ng/ml and 0.5 ng/ml, respectively. Finally, the method was applied to the determination of sulpiride in human serum samples obtained after a single oral dose of sulpiride.
authors.affiliation: Showa Pharmaceutical University, Machida 194-8543, Japan. chiba@ac.shoyaku.ac.jp
authors.forename: Ryoko
authors.initials: R
authors.lastname: Chiba
authors.forename: Ayako
authors.initials: A
authors.lastname: Ogasawara
authors.forename: Teppei
authors.initials: T
authors.lastname: Kubo
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yamazaki
authors.forename: Masuo
authors.initials: M
authors.lastname: Umino
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Ishizuka
mesh_terms: D001549:Benzamides / Q000097:blood*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D001549:Benzamides