title: Molecular cloning and characterization of a novel human J-domain protein gene (HDJ3) from the fetal brain.
abstract: The J-domain is believed to be part of a chaperone involved in protein folding. From a fetal brain cDNA library, we isolated a cDNA of 3249 bp encoding a novel human J-domain protein, which was named as HDJ3. The expression pattern of HDJ3 was examined by reverse transcription/polymerase chain reaction, which suggested that the transcripts were highly expressed in human pancreas and selectively expressed in human brain, lung, liver, skeletal muscle and kidney. The results also showed that a probable splice variant of HDJ3 gene might exist. The HDJ3 gene was located on human chromosome 12q13.1-12q13.2 and consisted of seven exons spanning 8593 bp of the human genome. PSORT analysis indicated that the HDJ3 gene contained a transmembrane domain. The putative protein of the HDJ3 gene was highly homologous to rat dopamine-receptor-interacting protein, suggesting that it was a novel member of the molecular chaperone family and functionally related to dopamine signal transduction.
authors.affiliation: Department of Neurosurgery, Chang Zheng Hospital, Second Military Medical University, 200003, Shanghai, People's Republic of China.
authors.forename: Juxiang
authors.initials: J
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, 200433, Shanghai , P.R. China.|United Gene Holdings, 200092, Shanghai, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, 200433, Shanghai , P.R. China.
authors.forename: Hai
authors.initials: H
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, 200433, Shanghai , P.R. China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Ni
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, 200433, Shanghai , P.R. China.
authors.forename: Haipeng
authors.initials: H
authors.lastname: Cheng
authors.affiliation: Department of Neurosurgery, Chang Zheng Hospital, Second Military Medical University, 200003, Shanghai, People's Republic of China.
authors.forename: Jingping
authors.initials: J
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, 200433, Shanghai , P.R. China.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Gu
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, 200433, Shanghai , P.R. China.
authors.forename: Xing
authors.initials: X
authors.lastname: Gu
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, 200433, Shanghai , P.R. China.
authors.forename: Gentao
authors.initials: G
authors.lastname: Cao
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, 200433, Shanghai , P.R. China.
authors.forename: Kang
authors.initials: K
authors.lastname: Ying
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, 200433, Shanghai , P.R. China.
authors.forename: Yumin
authors.initials: Y
authors.lastname: Mao
authors.affiliation: Department of Neurosurgery, Chang Zheng Hospital, Second Military Medical University, 200003, Shanghai, People's Republic of China.
authors.forename: Yicheng
authors.initials: Y
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, 200433, Shanghai , P.R. China. yxie@fudan.edu.cn.
authors.forename: Yi
authors.initials: Y
authors.lastname: Xie
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D002881:Chromosomes, Human, Pair 12; D003001:Cloning, Molecular; D005326:Fetal Proteins / Q000235:genetics*; D005333:Fetus; D015870:Gene Expression; D006801:Humans; D018832:Molecular Chaperones / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009928:Organ Specificity; D010179:Pancreas / Q000378:metabolism; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid
chemical_list: C476535:DNAJC14 protein, human; D005326:Fetal Proteins; D018832:Molecular Chaperones