[Study on regulatory effects of interleukin-6 on placental growth factor expression in trophoblast cells].
OBJECTIVE
To investigate the expression pattern of Placental growth factor (PlGF) and regulatory effects of interleukin-6 (IL-6) on PlGF expression in the primary cultured trophoblast cells.


METHODS
Cytotrophoblast cells were collected by trypsin-collagenase digestion and Percoll gradient centrifugation for primary culture. Reverse transcriptase polymerase chain reaction (RT-PCR) were resorted to demonstrate the expression pattern of PlGF mRNA in trophoblast cells cultured in vitro. The effects of IL-6 with different concentration (100, 10, 1 and 0.1 microg/L) and duration (6, 12, 24 and 48 h) on PlGF expression were observed.


RESULTS
The 185 bp and 248 bp bands of PlGF were showed by RT-PCR. PlGF expression correlated with IL-6. PlGF began to increase at 6 h, and reached the climax at 12 h when recultured with 100 microg/L IL-6.


CONCLUSIONS
PlGF expression has time and dose dependence on IL-6. It may play an important role in early pregnancy.
Peng
Wen
W
Department of Obstetrics and Gynecology, Shandong Provincial Hospital, Jinan 250021, China.
Jiang
Sen
S
Dai
Min-sheng
MS
D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000494:pharmacology*; D000072483:Placenta Growth Factor; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014327:Trophoblasts / Q000166:cytology / Q000378:metabolism*
D015850:Interleukin-6; C000608036:PGF protein, human; D011257:Pregnancy Proteins; D012333:RNA, Messenger; D000072483:Placenta Growth Factor