[Role of P53, nm23 proteins and vascular endothelial growth factor in angiogenesis and metastasis of laryngeal cancer].
OBJECTIVE
To elucidate the role of P53, nm23 proteins and vascular endothelial growth factor (VEGF) on angiogenesis and metastasis of laryngeal cancer.


METHODS
Specimens of 42 laryngeal carcinomas were studied by immunohistochemical staining for P53 protein, nm23 protein, VEGF and CD34 antibody.


RESULTS
Positive expressions of P53 protein, nm23 protein and VEGF were found in 47.6%, 57.1% and 71.42% of laryngeal cancer specimens respectively. There was a positive relationship between P53 and VEGF expression. The microvessel density (MVD) in P53- or VEGF--positive tumors was significantly higher in patients with cervical lymph node metastases than those without metastases. No correlation was identified between nm23 and VEGF staining. The MVD in nm23-negative and VEGF--positive tumors was significantly high. These phenomena were more easily found in patients with cervical lymph node metastases than in patients without metastases.


CONCLUSIONS
P53 gene play roles in the metastasis of laryngeal cancer through regulating VEGF expression and reacting MVD in tumor; and nm23 gene reacts through approaches other than regulating VEGF expression.
Cheng
W
W
Department of Otorhinolaryngology Head and Neck Surgery, First Clinical College, Norman Bethune University of Medical Sciences, Changchun 130021, China.
Li
X
X
Quan
C
C
Guo
X
X
D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000098:blood supply* / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D054778:NM23 Nucleoside Diphosphate Kinases; D009389:Neovascularization, Pathologic / Q000378:metabolism* / Q000473:pathology; D009701:Nucleoside-Diphosphate Kinase; D014176:Protein Biosynthesis; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis*; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis*
D054778:NM23 Nucleoside Diphosphate Kinases; D016159:Tumor Suppressor Protein p53; D042461:Vascular Endothelial Growth Factor A; C517938:NME1 protein, human; D009701:Nucleoside-Diphosphate Kinase