Effects of food on ethanol metabolism.
The goals of the present study were (1) to obtain ethanol pharmacokinetic data from fed dogs, and (2) perform Monte Carlo simulation to determine the effect of food on pharmacokinetic model parameter values. To a cohort of five fed dogs, 1 g ethanol per km body weight was administered as a gavage of 20% w/v ethanol solution. Blood samples taken at 0, 10, 20, 30, 40, 60, 80, 100, 120, 180, 240, and 360 minutes after the dose were mixed with anticoagulant and stored on ice. Blood ethanol concentration was determined via headspace chromatograph. Monte Carlo simulation with an ethanol pharmacokinetic model was used to estimate model parameter values and parameter standard deviations by minimization of the chi-squared function. Results indicate that 50.6 +/- 21.0% of the ethanol dose was absorbed in the stomach, and an insignificant amount of ethanol was metabolized by gastric alcohol dehydrogenase postulated for the model. At 6 hours after the ethanol dose 59.4 +/- 21.0% of the ethanol dose was retained in the dogs' stomachs.
Whitmire
David
D
Chemical Engineering, Tennessee Technological University, Cookeville, TN 38505, USA. dwhitmire@tntech.edu
Cornelius
Larry
L
Whitmire
Paula
P
D000426:Alcohol Dehydrogenase / Q000378:metabolism; D000818:Animals; D004285:Dogs; D000431:Ethanol / Q000097:blood / Q000493:pharmacokinetics*; D005502:Food; D018565:Food-Drug Interactions; D005746:Gastric Emptying; D005753:Gastric Mucosa / Q000378:metabolism; D007408:Intestinal Absorption; D007421:Intestine, Small / Q000201:enzymology; D009010:Monte Carlo Method; D013270:Stomach / Q000201:enzymology
D000431:Ethanol; D000426:Alcohol Dehydrogenase