title: [Preliminary study on NS5 region gene sequencing and variants of hepatitis G virus in China].
abstract: OBJECTIVE
  To study the genomic specificity and variation of Hepatitis G virus(HGV) in China.
  
  
  METHODS
  The 141nt cDNA fragments locating at nonstructural region 5 (NS5) of HGV were obtained from the sera of 16 HGV infected patients by RT-nested-PCR using NS5 primers, then purified and directly sequenced by dideoxynucleotide chain termination method.
  
  
  RESULTS
  The sequence diversity near the N-terminus of the region between nt 7,820 and 7,960 was more marked than that near the C-terminus. Compared with the HGV-US isolate, the nt alterations of NS5 in Chinese isolates usually took place at nt 7,824, 7,892, 7,914, 7,917, 7,935 and in region nt 7,845-7,860, the rate of variability at one nt position was 0-89.5%. The nucleotide homology was 83.6%-95.7% (89.6% in average) and the homology of deduced amino acid sequence was 83.0-100%(91.2% in average).
  
  
  CONCLUSIONS
  The nucleotide and deduced amino acid sequences of NS5 of Chinese HGV are relatively conserved but are obviously different from the HGV and GBV-C reported in USA in this study.
authors.affiliation: Department of Immunology, 302 Hospital of PLA, Beijing 100039.
authors.forename: B
authors.initials: B
authors.lastname: Li
authors.forename: Z
authors.initials: Z
authors.lastname: Dong
authors.forename: Y
authors.initials: Y
authors.lastname: Cheng
mesh_terms: D000328:Adult; D005260:Female; D018178:Flaviviridae Infections / Q000821:virology*; D029407:GB virus C / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics*; D006525:Hepatitis, Viral, Human / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D017421:Sequence Analysis; D017361:Viral Nonstructural Proteins / Q000235:genetics
chemical_list: D017361:Viral Nonstructural Proteins