title: Biomimetic mineralization of charged collagen matrices: in vitro and in vivo study.
abstract: Polyanionic collagen matrix prepared by hydrolysis side chain amides of asparagine and glutamine was mineralized in vivo, without inflammatory response, biodegradation, or resorption, with calcium phosphate deposited in close resemblance to the D-periodicity of collagen fibrils assembly. In vitro results with the same material produced mineralized collagen fibers with a similar morphology and chemical characteristics, suggesting that amide hydrolysis may have introduced into this matrix, signs for the controlled mineralization of collagen fiber. TEM indicated that amide hydrolysis occurred near the OVERLAP and GAP zones, as suggested by the significant reduction in inter-band distances in these regions. The lack of an inflammatory response associated to the similar mineralization pattern observed in vivo and in vitro suggests not only the biomimetic behavior of polyanionic collagen matrix, but also its potential uses as scaffold for bone tissue reconstruction. Based on these results, a model for the in vitro mineralization was also proposed.
authors.affiliation: Departamento de Química e Física Molecular, Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil.
authors.forename: Gilberto
authors.initials: G
authors.lastname: Goissis
authors.forename: Silvana Vargas
authors.initials: SV
authors.lastname: da Silva Maginador
authors.forename: Virginia
authors.initials: V
authors.lastname: da Conceição Amaro Martins
mesh_terms: D000818:Animals; D000838:Anions; D001216:Asparagine / Q000737:chemistry; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry; D001842:Bone and Bones / Q000502:physiology*; D002130:Calcium Phosphates / Q000737:chemistry; D002417:Cattle; D003094:Collagen / Q000138:chemical synthesis* / Q000737:chemistry; D017102:Fracture Healing; D005973:Glutamine / Q000737:chemistry; D066298:In Vitro Techniques; D008297:Male; D008855:Microscopy, Electron, Scanning; D051381:Rats; D017208:Rats, Wistar; D013499:Surface Properties; D013977:Tibia; D014961:X-Ray Diffraction
chemical_list: D000838:Anions; D001672:Biocompatible Materials; D002130:Calcium Phosphates; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; D005973:Glutamine; D001216:Asparagine; C485838:calcium phosphate, monobasic, anhydrous; D003094:Collagen; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous