Fluorescence digital photomacrography.
Low-power photographs of brain sections have been needed for neuroanatomical studies. This paper describes a setup for fluorescence digital photomacrography. High-resolution digital images were obtained with commercially available digital cameras combined with an oblique fluorescence illumination and filters for excitation and emission. The image of an entire coronal section of the macaque brain could be taken with a single exposure. The present photographic system would be useful not only for neuroanatomical, but also for histological, embryological or pathological studies that require low-magnification fluorescence images.
Tokuno
Hironobu
H
Department of Brain Structure, Tokyo Metropolitan Institute for Neuroscience, Fuchu, 183-8526, Tokyo, Japan. tokuno@tmin.ac.jp
Hatanaka
Nobuhiko
N
Chiken
Satomi
S
D000818:Animals; D001921:Brain / Q000033:anatomy & histology*; D003201:Computers; D005451:Fluorescein Angiography; D005453:Fluorescence; D006652:Histological Techniques; D007091:Image Processing, Computer-Assisted; D008251:Macaca; D010781:Photography / Q000379:methods*; D012984:Software