[Effect of the purariae-isofiavones on estrogen level in normal and ovariectomized rats].
OBJECTIVE
To study the effect of purariae isoflavone on estrogen level in ovariectomized rats.


METHODS
80 rats were divided into four groups randomly, every group with 20 rats: 1. Control group; 2. Normal + purariae isoflavone group; 3. Ovariectomized group; 4. Ovariectomized + purariae isoflavone group. Estrogen level and gonadotropin-releasing hormone level of all rats were measured.


RESULTS
Thirty days after being ovariectomized, E2, E3 level was significantly lower than that of the first group(P < 0.05). But Testerone, FSH, LH, PRL and GH increased(P < 0.05). After being gastrogavaged with purariae-isolfavone for thirty days, Estrogen level and gonadotropin-relasing hormone level of the second group were significantly lower in various degree than those of normal control group (P < 0.05). But in ovariectomized rats, the estrogen level was recovered (P > 0.05). The gonadotropin-releasing hormone level was increased (P < 0.05).


CONCLUSIONS
Purariae-isoflavone can increase estrogen level to normal in ovariectomized rats by way of increasing the level of gonadotropin-releasing hormone. In normal rats, it has anti-estrogen effect.
Qi
Ben-ling
BL
Department of Gerontology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei, China. qibenling@hotmail.com
Qi
Ben-ming
BM
D000818:Animals; D004958:Estradiol / Q000097:blood*; D004964:Estriol / Q000097:blood; D004965:Estrogen Antagonists / Q000494:pharmacology*; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000097:blood*; D007529:Isoflavones / Q000302:isolation & purification / Q000494:pharmacology*; D010052:Ovariectomy; D010946:Plants, Medicinal / Q000737:chemistry*; D029908:Pueraria / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley
D004965:Estrogen Antagonists; D007529:Isoflavones; D007987:Gonadotropin-Releasing Hormone; D004958:Estradiol; D004964:Estriol; C033607:puerarin