[Use of phage libraries for the in vitro production of recombinant monoclonal antibodies of predetermined specificity].
The biotechnological generation of monoclonal antibodies of predetermined specificity has traditionally involved the production of hybridomas obtained by somatic cellular fusion of splenocytes from immunized animals with myeloma cell lines bearing selectable markers. Now, monoclonal antibodies could be genetically engineered thus bypassing all the natural systems for making antibodies. Filamentous bacteriophages provides a means to display and select large single chain fragments variable (scFv) repertoires created by cloning the natural rearranged V-immunoglobulin genes or introducing predetermined level of randomization into germline V-gene segments. In this article we demonstrated that by using a well characterized scFv phage synthetic library it is possible to generate in vitro recombinant human monoclonal antibodies directed to a large array of antigens showing different molecular weights, conformations and origins.
Flego
Michela
M
Laboratorio di Immunologia, Istituto Superiore di Sanità, V.le Regina Elena 299, 00161 Roma.
Ascione
Alessandro
A
Pini
Alessandro
A
Mennella
Vito
V
Dupuis
Maria Luisa
ML
Benagiano
Giuseppe
G
Cianfriglia
Maurizio
M
D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000737:chemistry; D000918:Antibody Specificity; D001709:Biotechnology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D019151:Peptide Library; D011994:Recombinant Proteins / Q000096:biosynthesis
D000911:Antibodies, Monoclonal; D019151:Peptide Library; D011994:Recombinant Proteins