[Determination of cirsilineol in Herba Artemisiae scopariae collected in autumn by HPLC].
OBJECTIVE
To establish a quantitative method for determination of cirsilineol in Herba Artemisiae Scopariae collected in Autumn.


METHODS
Sample was extracted with methanol, and cleaned up with polyamide column. ODS column was used with methanol-acetonitrile-0.5% acetic acid solution (24:19:57) as mobile phase. Detection wavelength was 347 nm.


RESULTS
Cirsilineol in sample solution was well separated. Linearity of cirsilineol was good (r = 0.9998) in range of 0.1-0.8 microgram. The average recovery was 101.3%, RSD of repeatability was 3.04%.


CONCLUSIONS
This method can be used for quality control of Herba Artemisiae Scopariae collected in autumn and its preparations.
Zhang
Qi-wei
QW
Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
Zhang
Yong-xin
YX
Zhang
Ying
Y
Liang
Guo-gang
GG
D019306:Artemisia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D047309:Flavones; D005419:Flavonoids / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D012621:Seasons
D047309:Flavones; D005419:Flavonoids; C057618:cirsilineol