title: [Determination of trigonelline in Trigonella foenum-graecum by HPLC].
abstract: OBJECTIVE
  A HPLC method is established to determine the content of trigonelline in Trigonella foenum-graecum.
  
  
  METHODS
  The medicinal material was extracted by petholeum ether-ethanol. Asahipak NH2P-50 column was used, mobilephase consisted of acetonitrile-water(75:25) and detection wavelength was set at UV 265 nm.
  
  
  RESULTS
  The standard curve was linear in the range of 3.68-73.60 micrograms.mL-1 with the correlation coefficient of 0.9999. The average recovery rate and RSD were 97.4% and 1.83% (n = 6) respectively.
  
  
  CONCLUSIONS
  It provides scientific indexes for quality control of T. foenum-graecum.
authors.affiliation: Shenyang Pharmaceutical University, Pharmaceutical College, Shenyang 110015, Liaoning, China.
authors.forename: Huai-qing
authors.initials: HQ
authors.lastname: Zhao
authors.forename: Yan
authors.initials: Y
authors.lastname: Qu
authors.forename: Xue-ya
authors.initials: XY
authors.lastname: Wang
authors.forename: Hong-jian
authors.initials: HJ
authors.lastname: Zhang
authors.forename: Fa-mei
authors.initials: FM
authors.lastname: Li
authors.forename: Hattori
authors.initials: H
authors.lastname: Masao
mesh_terms: D000470:Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D012639:Seeds / Q000737:chemistry; D029910:Trigonella / Q000737:chemistry*
chemical_list: D000470:Alkaloids; C009560:trigonelline