[Determination of curculigoside in crude medicine Curculigo orchioides by HPLC].
OBJECTIVE
To establish a method for the determination of curculigoside in Curculigo orchioides, a species of crude medicine.


METHODS
The methanol was used as the solvent of exatraction and the curculigoside was extracted from the crude medicine with the method of the ultrasonic vibration. Using Sep-Pak C18 cartridges to purify the solution, the curculigoside was detected by HPLC. Intersil ODS-3(150 mm x 4.6 mm, 5 microns) chromatographic column was used, mobile phase of methanol-water-ice acetic acid (45:80:1) and detect wavelength was set at UV 283 nm.


RESULTS
The average recovery was 99.2% for the determination of curculigoside, RSD = 1.7% (n = 5). The content range of the curculigoside for 6 kinds of different samples was from 0.11% to 0.35%.


CONCLUSIONS
This method could be used to control the quality of crude medicine C. orchioides.
Lu
Hui-wen
HW
Guangdong Provincial Institute for Drug Control, Guangzhou 510180, Guangdong, China.
Zhu
Bing-hui
BH
Liang
Ying-kang
YK
D001565:Benzoates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D031408:Curculigo / Q000737:chemistry*; D005960:Glucosides / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D027343:Rhizome / Q000737:chemistry
D001565:Benzoates; D005960:Glucosides; C477170:curculigoside