[Structural features of a neutral heteropolysaccharide CPB-4 from Cynanchum paniculatum].
OBJECTIVE
To study the chemical features of CPB-4, a heteropolysaccharide obtained from Cynanchum paniculatum.


METHODS
Sugar composition analysis, methylation analysis, partial hydrolysis and carbon-13 nuclear magnetic resonance were used to determine the sugar composition, linkages, main chain, branch chains and branching points.


RESULTS
CPB-4 is composed of L-arabinose, L-xylose, L-rhamnose and D-galactose in closely molar ratios of 0.8:0.2:0.2:1.0. Its main chain is comprised of 1, 5 linked galactose and side chains are comprised of terminal xylose, terminal arabinose, oligosaccharide of arabinose and oligosaccharide of arabinose, rhamnose and galactose. The branching points are located at C-6 and C-2 of galactose.


CONCLUSIONS
CPB-4 is a new heteropolysaccharide from C. paniculatum.
Wang
Shun-chun
SC
Shanghai Institute of Materia Medica, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Bao
Xing-feng
XF
Fang
Ji-nian
JN
D001089:Arabinose / Q000302:isolation & purification; D031105:Cynanchum / Q000737:chemistry*; D008745:Methylation; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D012210:Rhamnose / Q000302:isolation & purification; D014994:Xylose / Q000302:isolation & purification
D011134:Polysaccharides; D014994:Xylose; D001089:Arabinose; D012210:Rhamnose