title: Comparison of platelet, leukocyte, and growth factor levels in point-of-care platelet-enriched plasma, prepared using a modified Curasan kit, with preparations received from a local blood bank.
abstract: The potential use of autologous thrombocytic growth factors to accelerate bone regeneration requires improved methods of isolating platelet-rich plasma (PRP). In addition to discontinuous cell separation, a second method by which PRP is produced at the point-of-care has now become available. In this study, growth factor levels in PRP from these two sources were compared. Whole blood was drawn from 115 healthy donors (73 males, 42 females) aged 21 - 62 years (mean 36, SD 10). The PRP was separated by the blood bank (BB) using the discontinuous cell separation method or at the 'point-of-care' by the so-called 'buffy coat' method (analogous to the Curasan PRP Kit). Growth factor content differed significantly for TGF-beta1 (BB 268.65+/-70.77 ng/ml, Curasan 95.02+/-60.67 ng/ml (sign test P<0.001)) and PDGF-AB (BB 133.59+/-46.26 ng/ml, Curasan 233.70+/-111.86 ng/ml (P<0.001)), while the content of IGF-I (BB 85.37+/-25.58 ng/ml, Curasan 101.72+/-47.7 ng/ml (P<0.160)) showed no significant difference. The higher thrombocyte count in the BB PRP (BB 1434300+/-351960/ microl, Curasan 908.500+/-492.30/microl) seems to result in higher TGF-beta1 levels, while the higher leukocyte count in the Curasan PRP (BB 160+/-320/ microl, Curasan 30130+/-12500/microl) seems to result in higher PDGF-AB levels. The similar IGF-I levels in the two preparations might merely reflect similar amounts of plasma in the PRP produced by each approach.
authors.affiliation: Clinic for Oral and Maxillofacial Surgery, Institute of Clinical Chemistry and Laboratory Medicine, Mainz, Germany. weibrich@mkg.klinik.uni-mainz.de
authors.forename: Gernot
authors.initials: G
authors.lastname: Weibrich
authors.forename: Wilfried K G
authors.initials: WK
authors.lastname: Kleis
authors.forename: Gerd
authors.initials: G
authors.lastname: Hafner
authors.forename: W E
authors.initials: WE
authors.lastname: Hitzler
authors.forename: Wilfried
authors.initials: W
authors.lastname: Wagner
mesh_terms: D000328:Adult; D001771:Blood Banks; D001800:Blood Specimen Collection; D002469:Cell Separation; D005260:Female; D006133:Growth Substances / Q000097:blood*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000032:analysis; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D010976:Platelet Count; D017713:Platelet Transfusion; D010982:Platelet-Derived Growth Factor / Q000032:analysis; D019095:Point-of-Care Systems; D018709:Statistics, Nonparametric; D016212:Transforming Growth Factor beta / Q000032:analysis; D053773:Transforming Growth Factor beta1
chemical_list: D006133:Growth Substances; D010982:Platelet-Derived Growth Factor; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; C095477:platelet-derived growth factor AB; D007334:Insulin-Like Growth Factor I