title: NOS: the underlying mechanism preserving vascular integrity and during ex vivo warm kidney perfusion.
abstract: Research involving metabolically active and functioning organs, maintained ex vivo in culture-like conditions, could provide numerous opportunities for medical innovations and research. We report successful perfusion of isolated canine and human kidneys ex vivo at near physiologic temperature for 48 h. During the perfusions parameters of metabolism and function remained stable. Nitric oxide synthase (NOS) was identified as the underlying mechanism preserving vascular integrity. Most importantly, when the canine kidneys were reimplanted there was immediate normal renal function. This report highlights the potential significance of whole organ culture using a warm temperature ex vivo perfusion and discusses medical applications that could be developed.
authors.affiliation: UM Faculty of Medicine and the Department of Surgery, Maastricht, the Netherlands.
authors.forename: Lauren
authors.initials: L
authors.lastname: Brasile
authors.forename: Bart M
authors.initials: BM
authors.lastname: Stubenitsky
authors.forename: Maurits H
authors.initials: MH
authors.lastname: Booster
authors.forename: Carl
authors.initials: C
authors.lastname: Haisch
authors.forename: Gauke
authors.initials: G
authors.lastname: Kootstra
mesh_terms: D000818:Animals; D004285:Dogs; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D016030:Kidney Transplantation; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D009924:Organ Culture Techniques; D010477:Perfusion
chemical_list: D019001:Nitric Oxide Synthase