title: Mutations of the BAK gene are infrequent in advanced gastric adenocarcinomas in Koreans.
abstract: The bcl-2 homologue antagonist/killer (BAK) is a potently apoptosis-inducing gene and plays an important role in modulating apoptosis in epithelial cells. We have analyzed the mutation of the entire coding region of BAK gene in 107 Korean advanced gastric adenocarcinomas by polymerase chain reaction-single strand conformation polymorphism and sequencing. Homozygous deletions were not found in these samples. Only three cases of 107 gastric adenocarcinomas (2.8%) exhibited the BAK mutations. Two of them exhibited missense mutations and the remaining one had a silent mutation. All of these mutations were exclusively detected in exon 2. Mutations in the BAK gene were observed only in advanced gastric adenocarcinomas with extensive metastases of regional lymph nodes. The data presented here suggest that the mutations of BAK gene rarely occurred in advanced gastric adenocarcinomas.
authors.affiliation: Department of Pathology, School of Medicine, Keimyung University, #194 DongSan Dong Jung-Gu, 700-712 Daegu, South Korea.
authors.forename: Sang-Pyo
authors.initials: SP
authors.lastname: Kim
authors.forename: Mi-Sun
authors.initials: MS
authors.lastname: Hwang
authors.forename: Young Rok
authors.initials: YR
authors.lastname: Cho
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Kwon
authors.forename: Yu-Na
authors.initials: YN
authors.lastname: Kang
authors.forename: In-Ho
authors.initials: IH
authors.lastname: Kim
authors.forename: Soo-Sang
authors.initials: SS
authors.lastname: Sohn
authors.forename: Kyo-Cheol
authors.initials: KC
authors.lastname: Mun
authors.forename: Taeg-Kyu
authors.initials: TK
authors.lastname: Kwon
authors.forename: Seong-Ryong
authors.initials: SR
authors.lastname: Lee
authors.forename: Seong-Il
authors.initials: SI
authors.lastname: Suh
mesh_terms: D000230:Adenocarcinoma / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology; D002288:Adenocarcinoma, Mucinous / Q000453:epidemiology / Q000235:genetics / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis / Q000235:genetics; D018279:Carcinoma, Signet Ring Cell / Q000453:epidemiology / Q000235:genetics / Q000473:pathology; D005091:Exons / Q000235:genetics; D005260:Female; D006801:Humans; D007723:Korea / Q000453:epidemiology; D008207:Lymphatic Metastasis; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D008875:Middle Aged; D020125:Mutation, Missense; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D013274:Stomach Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology; D050998:bcl-2 Homologous Antagonist-Killer Protein
chemical_list: C494772:BAK1 protein, human; D008565:Membrane Proteins; D050998:bcl-2 Homologous Antagonist-Killer Protein