title: [Determination of curculigoside in crude medicine Curculigo orchioides by HPLC].
abstract: OBJECTIVE
  To establish a method for the determination of curculigoside in Curculigo orchioides, a species of crude medicine.
  
  
  METHODS
  The methanol was used as the solvent of exatraction and the curculigoside was extracted from the crude medicine with the method of the ultrasonic vibration. Using Sep-Pak C18 cartridges to purify the solution, the curculigoside was detected by HPLC. Intersil ODS-3(150 mm x 4.6 mm, 5 microns) chromatographic column was used, mobile phase of methanol-water-ice acetic acid (45:80:1) and detect wavelength was set at UV 283 nm.
  
  
  RESULTS
  The average recovery was 99.2% for the determination of curculigoside, RSD = 1.7% (n = 5). The content range of the curculigoside for 6 kinds of different samples was from 0.11% to 0.35%.
  
  
  CONCLUSIONS
  This method could be used to control the quality of crude medicine C. orchioides.
authors.affiliation: Guangdong Provincial Institute for Drug Control, Guangzhou 510180, Guangdong, China.
authors.forename: Hui-wen
authors.initials: HW
authors.lastname: Lu
authors.forename: Bing-hui
authors.initials: BH
authors.lastname: Zhu
authors.forename: Ying-kang
authors.initials: YK
authors.lastname: Liang
mesh_terms: D001565:Benzoates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D031408:Curculigo / Q000737:chemistry*; D005960:Glucosides / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D027343:Rhizome / Q000737:chemistry
chemical_list: D001565:Benzoates; D005960:Glucosides; C477170:curculigoside