title: Common cytological and cytogenetic features of Epstein-Barr virus (EBV)-positive natural killer (NK) cells and cell lines derived from patients with nasal T/NK-cell lymphomas, chronic active EBV infection and hydroa vacciniforme-like eruptions.
abstract: In this study, we describe the cytological and cytogenetic features of six Epstein-Barr virus (EBV)-infected natural killer (NK) cell clones. Three cell clones, SNK-1, -3 and -6, were derived from patients with nasal T/NK-cell lymphomas; two cell clones, SNK-5 and -10, were isolated from patients with chronic active EBV infection (CAEBV); and the other cell clone, SNK-11, was from a patient with hydroa vacciniforme (HV)-like eruptions. An analysis of the number of EBV-terminal repeats showed that the SNK cell clones had monoclonal EBV genomes identical to the original EBV-infected cells of the respective patients, and SNK cells had the type II latency of EBV infection, suggesting that not only the cell clones isolated from nasal T/NK-cell lymphomas but also those isolated from CAEBV and HV-like eruptions had been transformed by EBV to a certain degree. Cytogenetic analysis detected deletions in chromosome 6q in five out of the six SNK cell clones, while 6q was not deleted in four control cell lines of T-cell lineage. This suggested that a 6q deletion is a characteristic feature of EBV-positive NK cells, which proliferated in the diseased individuals. The results showed that EBV-positive NK cells in malignant and non-malignant lymphoproliferative diseases shared common cytological and cytogenetic features.
authors.affiliation: Department of Virology, Division of Virology and Immunology, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nagata
authors.forename: Tatsuro
authors.initials: T
authors.lastname: Ikeuchi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Mukai
authors.forename: Michiko K
authors.initials: MK
authors.lastname: Oyoshi
authors.forename: Ayako
authors.initials: A
authors.lastname: Demachi
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Morio
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Wakiguchi
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Kimura
authors.forename: Norio
authors.initials: N
authors.lastname: Shimizu
authors.forename: Kohtaro
authors.initials: K
authors.lastname: Yamamoto
mesh_terms: D000293:Adolescent; D000328:Adult; D015153:Blotting, Western; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics; D002908:Chronic Disease; D002999:Clone Cells; D020031:Epstein-Barr Virus Infections / Q000235:genetics / Q000473:pathology*; D005260:Female; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D006837:Hydroa Vacciniforme / Q000235:genetics / Q000473:pathology*; D007694:Killer Cells, Natural / Q000821:virology*; D016399:Lymphoma, T-Cell / Q000235:genetics / Q000473:pathology; D008232:Lymphoproliferative Disorders / Q000235:genetics / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009669:Nose Neoplasms / Q000235:genetics / Q000473:pathology*