title: The human G protein beta4 subunit: gene structure, expression, Ggamma and effector interaction.
abstract: The aim of this study was the characterization of the human Gbeta4 subunit of heterotrimeric G proteins. Human Gbeta4 is widely expressed. Its gene is located on chromosome 3 with a genomic structure indistinguishable from that of the genes of Gbeta1 to Gbeta3, but entirely different from Gbeta5. In vitro translation co-precipitation analyses revealed that Gbeta4 can form stable dimers with Ggamma1, Ggamma2, Ggamma3, Ggamma4, Ggamma5, Ggamma7, Ggamma10, Ggamma11, Ggamma12, and Ggamma13, dimers which were also able to stimulate phospholipase beta2.
authors.affiliation: Institut für Pharmakologie, Universitätsklinikum Essen, Hufelandstr. 55, Germany. dieter.rosskopf@uni-essen.de
authors.forename: Dieter
authors.initials: D
authors.lastname: Rosskopf
authors.forename: Christiane
authors.initials: C
authors.lastname: Nikula
authors.forename: Iris
authors.initials: I
authors.lastname: Manthey
authors.forename: Markus
authors.initials: M
authors.lastname: Joisten
authors.forename: Ulrich
authors.initials: U
authors.lastname: Frey
authors.forename: Stefanie
authors.initials: S
authors.lastname: Kohnen
authors.forename: Winfried
authors.initials: W
authors.lastname: Siffert
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002893:Chromosomes, Human, Pair 3; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D019281:Dimerization; D004789:Enzyme Activation; D005091:Exons; D020962:Heterotrimeric GTP-Binding Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D008957:Models, Genetic; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D014018:Tissue Distribution
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D020962:Heterotrimeric GTP-Binding Proteins