title: Electron transfer in human cytochrome P450 reductase.
abstract: Cytochrome P450 reductase (CPR) is a diflavin enzyme responsible for electron donation to mammalian cytochrome P450 enzymes in the endoplasmic reticulum. Dissection of the enzyme into functional domains and studies by site-directed mutagenesis have enabled detailed characterization of the mechanism of electron transfer using stopped-flow and equilibrium-perturbation methods, and redox potentiometry. These studies and the mechanism of electron transfer in CPR are reported herein.
authors.affiliation: Department of Biochemistry, University of Leicester, University Road, Leicester LE1 7RH, UK.
authors.forename: A
authors.initials: A
authors.lastname: Gutierrez
authors.forename: A
authors.initials: A
authors.lastname: Grunau
authors.forename: M
authors.initials: M
authors.lastname: Paine
authors.forename: A W
authors.initials: AW
authors.lastname: Munro
authors.forename: C R
authors.initials: CR
authors.lastname: Wolf
authors.forename: G C K
authors.initials: GC
authors.lastname: Roberts
authors.forename: N S
authors.initials: NS
authors.lastname: Scrutton
mesh_terms: D000595:Amino Acid Sequence; D004579:Electron Transport; D006801:Humans; D007700:Kinetics; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism*; D014364:Tryptophan
chemical_list: D014364:Tryptophan; D009251:NADPH-Ferrihemoprotein Reductase