Phosphodiesterase 4 in osteoblastic osteosarcoma cells as a potential target for growth inhibition.
Reverse transcription-polymerase chain reaction (RT-PCR) analysis revealed that HOSM-1 cells, an osteosarcoma cell line established from human mandible, expressed mRNA for osteoblastic markers, such as alkaline phosphatase, osteonectin, osteocalcin and parathyroid hormone receptor, thus exhibiting an osteoblastic phenotype. We have investigated a possible role of cyclic nucleotide phosphodiesterases (PDEs) in osteosarcoma cells. RT-PCR analysis revealed that HOSM-1 cells expressed mRNA for PDE4A, 4B and 4C. In addition, rolipram, a specific inhibitor of PDE4, inhibited HOSM-1 cell proliferation. The finding that PDE4 is involved in proliferation of osteosarcoma cells suggests the possibility that PDE4 may be a new target for antitumor therapy.
Narita
Motoshi
M
Department of Oral and Maxillofacial Surgery, Faculty of Medicine, Mie University, Tsu, Japan. nya@clin.medic.mie-u.ac.jp
Murata
Taku
T
Shimizu
Kasumi
K
Sugiyama
Takeo
T
Nakagawa
Toshiyuki
T
Manganiello
Vincent C
VC
Tagawa
Toshiro
T
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000235:genetics / Q000378:metabolism*; D000970:Antineoplastic Agents; D001859:Bone Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D054703:Cyclic Nucleotide Phosphodiesterases, Type 4; D006801:Humans; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000378:metabolism; D012516:Osteosarcoma / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D012334:RNA, Neoplasm / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020889:Rolipram / Q000494:pharmacology
D000970:Antineoplastic Agents; D007527:Isoenzymes; D010726:Phosphodiesterase Inhibitors; D012334:RNA, Neoplasm; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D054703:Cyclic Nucleotide Phosphodiesterases, Type 4; C517355:PDE4A protein, human; D020889:Rolipram