title: [Preparation of genistein-loaded chitosan microspheres].
abstract: OBJECTIVE
  To optimize the preparation of genistein chitosan microspheres with central composite design (CCD).
  
  
  METHODS
  The chitosan microspheres were prepared by the O/W/O multiple emulsion method. Second-order polynomial and linear equations were fitted to the data, and the resulting equations were used to produce response surface graphs and the best experiment conditions.
  
  
  RESULTS
  The theoretical drug content was 13%-15%, the concentration of organic phase was 30%-40% and the concentration of oil phase was 68%-72%.
  
  
  CONCLUSIONS
  The best experiment conditions can be obtained by central composite design and response surface methodology. The observed values agree well with model predicted values.
authors.affiliation: College of Pharmacy, Beijing University of Traditional Chinese Medicine, Beijing 100029, China.
authors.forename: Wan-ying
authors.initials: WY
authors.lastname: Wu
authors.forename: Yun-gu
authors.initials: YG
authors.lastname: Li
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage*; D002686:Chitin / Q000031:analogs & derivatives*; D048271:Chitosan; D003692:Delayed-Action Preparations; D019833:Genistein / Q000008:administration & dosage*; D008433:Mathematics; D008863:Microspheres
chemical_list: D000970:Antineoplastic Agents; D003692:Delayed-Action Preparations; D002686:Chitin; D048271:Chitosan; D019833:Genistein