Predicting survival in patients with metastatic kidney cancer by gene-expression profiling in the primary tumor.
To identify potential molecular determinants of tumor biology and possible clinical outcomes, global gene-expression patterns were analyzed in the primary tumors of patients with metastatic renal cell cancer by using cDNA microarrays. We used grossly dissected tumor masses that included tumor, blood vessels, connective tissue, and infiltrating immune cells to obtain a gene-expression "profile" from each primary tumor. Two patterns of gene expression were found within this uniformly staged patient population, which correlated with a significant difference in overall survival between the two patient groups. Subsets of genes most significantly associated with survival were defined, and vascular cell adhesion molecule-1 (VCAM-1) was the gene most predictive for survival. Therefore, despite the complex biological nature of metastatic cancer, basic clinical behavior as defined by survival may be determined by the gene-expression patterns expressed within the compilation of primary gross tumor cells. We conclude that survival in patients with metastatic renal cell cancer can be correlated with the expression of various genes based solely on the expression profile in the primary kidney tumor.
Vasselli
James R
JR
Urologic Oncology Branch, National Cancer Institute, Bethesda, MD 20892, USA.
Shih
Joanna H
JH
Iyengar
Shuba R
SR
Maranchie
Jodi
J
Riss
Joseph
J
Worrell
Robert
R
Torres-Cabala
Carlos
C
Tabios
Ray
R
Mariotti
Andra
A
Stearman
Robert
R
Merino
Maria
M
Walther
McClellan M
MM
Simon
Richard
R
Klausner
Richard D
RD
Linehan
W Marston
WM
D000328:Adult; D000368:Aged; D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000401:mortality* / Q000556:secondary*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics* / Q000401:mortality*; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D015996:Survival Rate; D014481:United States / Q000453:epidemiology; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics
D019010:Vascular Cell Adhesion Molecule-1