Isoenzymatic variability among five Anopheles species belonging to the Nyssorhynchus and Anopheles subgenera of the Amazon region, Brazil.
An isoenzymatic comparative analysis of the variability and genetics differentiation among Anopheles species was done in populations of An. (Nys.) intermedius and An. (Ano.) mattogrossensis of the Anopheles subgenus, and of An. darlingi, An. albitarsis and An. triannulatus of the Nyssorhynchus subgenus, with the aim of detecting differences between both subgenera and of estimating the degree of genetic intere specific divergence. Samples from Macap , State of Amap  and Janauari Lake, near Manaus, State of Amazonas, were analyzed for eight isoenzymatic loci. Analysis revealed differences in the average number of alleles per locus (1.6-2.3) and heterozygosity (0.060-0.284). However, the proportion of polymorphic loci was the same for An. (Nys.) darlingi, An. (Nys.) triannulatus and An. (Ano.) mattogrossensis (50%), but differed for An. (Nys.) albitarsis (62.5%) and An. (Ano.) intermedius (25%). Only the IDH1 (P > 0.5) locus in all species studied was in Hardy-Weinberg equilibrium. The fixation index demonstrated elevated genetic structuring among species, based on values of Fst = 0.644 and genetic distance (0.344-0.989). Genetic difference was higher between An. (Nys.) triannulatus and An. (Ano.) intermedius (0.989) and smaller between An. (Nys.) albitarsis sensu lato and An. (Nys.) darlingi (0.344). The data show interspecific genetic divergence which differs from the phylogenetic hypothesis based on morphological characters.
dos Santos
Joselita Maria Mendes
JM
Instituto Nacional de Pesquisas da Amazônia, Manaus, AM, Brasil. jsantos@inpa.gov.br
Maia
Juracy de Freitas
Jde F
Tadei
Wanderli Pedro
WP
Rodriguez
Gloria Alícia Diaz
GA
D000483:Alleles; D000818:Animals; D000852:Anopheles / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D001938:Brazil; D017344:Genes, Insect / Q000235:genetics*; D014644:Genetic Variation; D007303:Insect Vectors / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D007527:Isoenzymes / Q000235:genetics*; D013045:Species Specificity
D007527:Isoenzymes