[Association of HMG-CoA reductase gene polymorphism with levels of lipids].
OBJECTIVE
To study the distribution of ScrF1 restriction polymorphism in intron 2 of the 3-hydroxy-3-methylglutaryl coenzyme A(HMG-CoA) reductase gene in Chinese Han population and the association of the polymorphism with coronary heart disease(CHD).


METHODS
HMG-CoA reductase genotyping was performed using polymerase chain reaction-restriction fragment polymorphism.


RESULTS
HMG-CoA reductase allelic frequencies of A, a were 0.519, 0.481; 0.440, 0.560 in CHD group and control group respectively. There was no significant difference in frequencies of allele and genotype in ScrF1 polymorphism between CHD group and control group(P>0.05). However, the levels of plasma very low density lipoprotein (VLDL) and TG in CHD patients with AA genotype were higher than those in CHD patients with other genotypes(P<0.05). The frequencies of A, a alleles at ScrF1 polymorphic site were significantly different from those reported in European Caucasians (0.44 vs 0.55, 0.56 vs 0.45, P<0.05).


CONCLUSIONS
No direct association was found between the ScrF1 polymorphism and CHD, but there is a significant correlation between the AA genotype of the HMG-CoA reductase gene and the levels of plasma VLDL and TG in CHD group.
Tong
Yu
Y
Department of Medical Genetics, West China Hospital, Sichuan University, Chengdu, Sichuan, PR China.
Zhang
Si-zhong
SZ
Su
Zhi-guang
ZG
Kong
Xiang-dong
XD
Shi
Jia-jun
JJ
Zhang
Li
L
Zhang
Heng-yu
HY
Zhang
Ke-lan
KL
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People / Q000235:genetics*; D001774:Blood Chemical Analysis; D015243:Cholesterol, VLDL / Q000097:blood; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D006903:Hydroxymethylglutaryl CoA Reductases / Q000235:genetics*; D025782:Hydroxymethylglutaryl-CoA-Reductases, NADP-dependent / Q000235:genetics; D050356:Lipid Metabolism / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D014280:Triglycerides / Q000097:blood
D015243:Cholesterol, VLDL; D014280:Triglycerides; D006903:Hydroxymethylglutaryl CoA Reductases; D025782:Hydroxymethylglutaryl-CoA-Reductases, NADP-dependent