Effect of carbon source on alkaline phosphatase production and excretion in Aspergillus caespitosus.
The effect of several carbon sources on the production of alkaline phosphatase by the thermotolerant Aspergillus caespitosus was analysed. The fungus released high levels of alkaline phosphatases into the medium after being cultured for long periods with xylan or industrial residues such as wheat raw and sugar cane bagasse in the culture media. In contrast, the alkaline phosphatase activities were found only intracellulary when the fungus was cultured in glucose-supplemented media. The pH of the medium likely affects the process of enzyme secretion according to the carbon source used. Addition of xylan or industrial residues in the culture medium stimulated the secretion of phosphatases. In contrast, media supplemented with glucose or disaccharides promoted retention of these enzymes into the cells. The subcellular location activities of alkaline phosphatases were studied using histochemical and immunochemical methods and showed that alkaline phosphatases were present in the mycelial walls and septa.
Guimarães
Luis Henrique Souza
LH
Departamento de Biologia da Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto - Universidade de São Paulo, Brazil.
Jorge
João Atilio
JA
Terenzi
Héctor Francisco
HF
Jamur
Maria Célia
MC
Oliver
Constance
C
De Lourdes Teixeira De Moraes Polizeli
Maria
M
D000469:Alkaline Phosphatase / Q000096:biosynthesis* / Q000378:metabolism; D001230:Aspergillus / Q000145:classification / Q000201:enzymology* / Q000378:metabolism; D018554:Avena / Q000378:metabolism; D002244:Carbon / Q000737:chemistry / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006863:Hydrogen-Ion Concentration; D007150:Immunohistochemistry; D013194:Staining and Labeling; D014908:Triticum / Q000378:metabolism; D014990:Xylans / Q000378:metabolism
D003470:Culture Media; D014990:Xylans; D002244:Carbon; D002482:Cellulose; C027433:bagasse; D000469:Alkaline Phosphatase