title: CO2 hydration by immobilized carbonic anhydrase.
abstract: The enzyme carbonic anhydrase (isoform II) from bovine and human erythrocytes was immobilized using different covalent coupling methods on inert matrices. Immobilized carbonic anhydrase may enable concentration of CO2 for Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase)-catalysed fixation in bioreactors. In the present study the activity of carbonic anhydrase with respect to hydration of CO2 using soluble and immobilized enzymes was determined. The stability of the immobilization matrix, the properties of the immobilized enzymes subjected to a variation in operation variables and the activity profile with respect to storage are reported. Immobilization imparted greater thermal and storage stability and enhanced reusability.
authors.affiliation: ABRD Company LLC, 1555 Wood Road, Cleveland, OH 44121, USA. bhattas@ccf.org
authors.forename: Sumana
authors.initials: S
authors.lastname: Bhattacharya
authors.forename: Marc
authors.initials: M
authors.lastname: Schiavone
authors.forename: Subhra
authors.initials: S
authors.lastname: Chakrabarti
authors.forename: Sanjoy K
authors.initials: SK
authors.lastname: Bhattacharya
mesh_terms: D000818:Animals; D002245:Carbon Dioxide / Q000737:chemistry / Q000378:metabolism*; D024402:Carbonic Anhydrase II / Q000737:chemistry / Q000378:metabolism*; D002417:Cattle; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D004912:Erythrocytes / Q000201:enzymology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D013696:Temperature; D014867:Water / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D014867:Water; D002245:Carbon Dioxide; D024402:Carbonic Anhydrase II