Analysis of DLC-1 expression in human breast cancer.
The chromosome region 8p12-p22 shows frequent allelic loss in many neoplasms, including breast cancer (BC). The DLC-1 gene, located on 8p21-p22, might be a candidate tumor suppressor gene in this region. To evaluate the involvement of DLC-1 in breast carcinogenesis we studied DLC-1 mRNA expression in a panel of 14 primary human BC and the corresponding normal breast cells as well as 8 BC cell lines. Low levels or absence of DLC-1 mRNA were observed in 57% of primary BC and 62.5% of BC cell lines, respectively. We could not find any correlation between DLC-1 mRNA expression and deletions at the DLC-1 locus. Transfection of the gene into DLC-1 deficient T-47D cells raised the DLC-1 mRNA level and resulted in inhibition of cell growth and reduced colony-forming capacity. Our results indicate a role of DLC-1 in BC carcinogenesis.
Plaumann
Marlies
M
Department of Tumor Genetics, Max Delbrück Center for Molecular Medicine, Robert-Rössle-Str. 10, 13092 Berlin-Buch, Germany. m.plaumann@mdc-berlin.de
Seitz
Susanne
S
Frege
Renate
R
Estevez-Schwarz
Lope
L
Scherneck
Siegfried
S
D001943:Breast Neoplasms / Q000235:genetics*; D002455:Cell Division; D002471:Cell Transformation, Neoplastic; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics*; D005260:Female; D020690:GTPase-Activating Proteins; D017353:Gene Deletion; D005786:Gene Expression Regulation; D006801:Humans; D019656:Loss of Heterozygosity; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis* / Q000235:genetics
C112352:DLC1 protein, human; D020690:GTPase-Activating Proteins; D012333:RNA, Messenger; D025521:Tumor Suppressor Proteins