[Measurement of baicalin contents by HPLC].
OBJECTIVE
To compare the contents of baicalin in the raw medical material and the different prepared medical materials of Scutellaria baicalensis.


METHODS
The contents of baicalin were determined by HPLC. Chromatographic conditions included Hypersil C18 column and the mobile phase consisting of a mixture of methanol-0.04% phosphoric acid (46:54). Baicalin was detected at 280 nm. The standard curve was linear in the range of 0.0280-4.6700 micrograms with correlation coefficient 1.0000. The average recovery of baicalin was 101.22% with RSD = 2.54% (n = 5).


RESULTS
The contents of baicalin were 6.80% in raw material, 6.00% and 6.73% in the different prepared medical materials of S. baicalensis respectively.


CONCLUSIONS
The two different kinds of preparing methods can be used to the raw material of S. baicalensis.
He
Zhu-ying
ZY
First Affiliated Hospital of Guiyang College of Traditional Chinese Medicine, Guiyang 550001, Guizhou, China.
Cao
Pei-xue
PX
Liang
Guang-yi
GY
Liu
Zai-chuan
ZC
Wu
Xing-guo
XG
D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis*; D006358:Hot Temperature; D010946:Plants, Medicinal / Q000737:chemistry*; D031364:Scutellaria baicalensis / Q000737:chemistry*; D013678:Technology, Pharmaceutical
D005419:Flavonoids; C038044:baicalin