Emergence of a new satellite RNA from cucumber mosaic virus isolate P1.
The cucumber mosaic virus (CMV) isolate P1 caused very mild symptoms on many plant species. After serial passages by mechanical inoculation over five years, CMV P1 caused severe symptoms on several tobacco cultivars and tomato. A specific band of approximately 0.3 kb in length was amplified by RT-PCR with primers synthesized based on reported CMV satellite RNA (satRNA) sequences. Sequence analysis showed there were two satRNAs (Sat-P1-1 and Sat-P1-2). Sat-P1-1 contained 335 nucleotides, and Sat-P1-2 contained 394 nucleotides. These two satRNAs shared 64% overall nucleotide sequence homology, and differences between the two satRNAs included mutations as well as deletions. Sat-P1-1 was identical to a satRNA (Z96099) reported in 1995 in CMV P1. Based on differences in the sequence and secondary structure between these two satRNAs, we conclude that Sat-P1-2 represents the emergence of a new satellite (necrotic satellite) from attenuated satRNA populations. The possible effect of the emergence of this new satRNA is discussed.
Pérez Alvarez
Sandra
S
Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China. sandraperez_2000@hotmail.com
Xue
Chao-Yang
CY
Zhou
Xue-Ping
XP
D001483:Base Sequence; D005075:Biological Evolution; D019401:Cucumber Mosaic Virus Satellite / Q000235:genetics*; D017799:Cucumovirus / Q000737:chemistry* / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D018532:Pisum sativum / Q000235:genetics / Q000821:virology*; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA
D019401:Cucumber Mosaic Virus Satellite