Malondialdehyde production in Jurkat T cells subjected to oxidative stress.
OBJECTIVE
We investigated the relation between membrane lipid peroxidation, as evaluated by malondialdehyde (MDA), and oxidative stimuli in the Jurkat T-cell line and designed a cellular model to assess the antioxidant potential of compounds.


METHODS
Jurkat T cells were subjected to different concentrations of Fe(2+) ions (from 25 to 150 micromol/L) or H(2)O(2) (from 0.1 to 5 mmol/L), and MDA was determined after separation in high-performance liquid chromatography of the adduct with thiobarbituric acid. MDA production also was investigated in cells supplemented with epigallocatechin gallate and genistein and subjected to Fe(2+) oxidative treatment.


RESULTS
MDA production increased with the concentration of Fe(2+), whereas H(2)O(2) had no effect at any concentration. Oxidative stress for 15 min or 2 h produced similar MDA levels. The supplementation of epigallocatechin gallate partly prevented MDA production (about 40%, P < 0.05), whereas genistein exerted no preventive effect on lipid peroxidation.


CONCLUSIONS
We propose this cellular model, consisting of Jurkat T cells subjected to 100 micromol/L of Fe(2+) for 15 min, to study the protective effect of antioxidant supplementation against membrane lipid peroxidation.
Erba
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Department of Food Science and Microbiology, University of Milan, Via Celoria 2, 20133 Milan, Italy. daniela.erba@unimi.it
Riso
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Criscuoli
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Testolin
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D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D005296:Ferrous Compounds / Q000008:administration & dosage; D019833:Genistein / Q000494:pharmacology; D006801:Humans; D006861:Hydrogen Peroxide / Q000008:administration & dosage; D019169:Jurkat Cells; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000032:analysis / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism; D018384:Oxidative Stress; D013601:T-Lymphocytes / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure
D005296:Ferrous Compounds; D008563:Membrane Lipids; D008315:Malondialdehyde; D002392:Catechin; D006861:Hydrogen Peroxide; C045651:epigallocatechin gallate; D019833:Genistein