Deoxyribozyme-based half-adder.
We have constructed a solution-phase array of three deoxyribozyme-based logic gates that behaves as a half-adder. Two deoxyribozymes mimic i(1)ANDNOTi(2) and i(2)ANDNOTi(1) gates that cleave a fluorogenic substrate, reporting through an increase in fluorescence emission at 570 nm. The third deoxyribozyme mimics an i(1)ANDi(2) gate and cleaves the other fluorogenic substrate, reporting through an increase in fluorescence emission at 520 nm. Together, this system represents the first example of a decision-making enzymatic network with two inputs and two outputs. Similar systems could be applied to control autonomous therapeutic and diagnostic devices.
Stojanović
Milan N
MN
Division of Clinical Pharmacology and Experimental Therapeutics, Department of Medicine, Columbia University, Box 84, 630 W 168th Street, New York, NY 10032, USA. mns18@columbia.edu
Stefanović
Darko
D
D001483:Base Sequence; D021881:DNA, Catalytic / Q000737:chemistry*; D008128:Logic; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D013050:Spectrometry, Fluorescence
D021881:DNA, Catalytic