Kindred DNA amplification from two distinct populations of cDNA fragments.
Kindred DNA amplification is a novel and cost-effective method developed to isolate common cDNA fragments between two distinct cDNA populations. Unlike subtractive hybridization, which discards common sequences, kindred DNA amplification isolates and amplifies these sequences within a single hybridization procedure. The utility of this method is demonstrated by cloning the genes in common between two different but metabolically homologous muscles, murine ventricular myocardium and soleus. The reliability of kindred DNA amplification was confirmed by Southern hybridization.
Puurand
Ulo
U
Department of Pathophysiology, Institute of General and Molecular Pathology, Faculty of Medicine, University of Tartu, 19 Ravila Street, Tartu 50411, Estonia. ulo@ut.ee
Kadaja
Lumme
L
Seppet
Enn K
EK
D000818:Animals; D046508:Culture Techniques; D053938:DNA Fragmentation; D018076:DNA, Complementary / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D020869:Gene Expression Profiling / Q000379:methods*; D014644:Genetic Variation / Q000235:genetics*; D005828:Genetics, Population / Q000379:methods*; D051379:Mice; D018482:Muscle, Skeletal / Q000378:metabolism; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012313:RNA / Q000235:genetics / Q000378:metabolism
D018076:DNA, Complementary; D012313:RNA