Long lasting cadmium intake is associated with reduction of insulin receptors in rat adipocytes.
The effects of chronic cadmium exposure on adipose tissue have not been extensively reported. In adult Wistar male rats we investigated in vivo effect of 6 weeks lasting cadmium intake in drinking tap water (CdCl2 9,7 mg/l). Insulin receptors in isolated adipocytes from epididymal fat and glucose transporter protein GLUT4 content in fat tissue plasma membranes were determined. Control and Cd treated rats had similar water intake with subsequent heavy augmentation of Cd content in liver of experimental animals. In comparison with controls, Cd intake did not influence body mass increment and fat cell size, but significantly increased serum glycemia and moderately elevated insulinemia. Cadmium intake significantly reduced (approximately 50%) both, total insulin receptors number and density of the receptors in fat cells. No differences in the content of GLUT4 in crude plasma membranes of adipose tissue were observed. Diminished insulin receptors in adipocytes could account for diabetogenic effect of long lasting cadmium intake.
Ficková
M
M
Institute of Experimental Endocrinology, Centre of Excellence of EU, 833 06 Bratislava, Slovakia. ueenfick@savba.sk
Eybl
V
V
Kotyzová
D
D
Micková
V
V
Möstbök
S
S
Brtko
J
J
D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D015153:Blotting, Western; D002104:Cadmium / Q000008:administration & dosage* / Q000494:pharmacology*; D004326:Drinking / Q000187:drug effects; D051275:Glucose Transporter Type 4; D007328:Insulin / Q000097:blood; D008297:Male; D009004:Monosaccharide Transport Proteins / Q000378:metabolism; D009124:Muscle Proteins; D051381:Rats; D017208:Rats, Wistar; D011972:Receptor, Insulin / Q000378:metabolism*; D015430:Weight Gain / Q000187:drug effects
D001786:Blood Glucose; D051275:Glucose Transporter Type 4; D007328:Insulin; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; C495694:Slc2a4 protein, rat; D002104:Cadmium; D011972:Receptor, Insulin