[Effect of transduction bax gene on experimental nasopharyngeal carcinoma].
OBJECTIVE
To evaluate the effect of transduction bax gene on nasopharyngeal carcinoma (NPC).


METHODS
The transduced bax gene was mediated by Dosper lipidsome to the HNE-1 cell lines, and confirmed by immunofluorescent stain. The apoptosis of the cell lines was studied by MTT stain and flow cytometry and the growth of implanted tumor in naked mouse after local injection of bax geneobserved.


RESULTS
Instantaneous expression of bax gene in HNE1 cell lines was testified by immunofluorescent stain after transduction for 48 hours, the apoptotic index were 10.7% and 69.4% for pre- and post-transduction, respectively. MTT stain showed the values of A in 490 nm after transduction to be 2.004(36 h) and 1.902(48 h). The diameter of implanted tumor mass was (1.8 +/- 0.64) cm and (3.0 +/- 0.44) cm in experimental and control groups, respectively.


CONCLUSIONS
The transduction bax gene can enhance the apoptosis of HNE1 cell lines and prevent growth of implanted tumor.
Li
H
H
Department of Otorhinolaryngology, Third Hospital of Sun-Yat-Sen University of Medical Sciences, Guangzhou 510630, China. lhb73@263.net
Xie
M
M
Xu
G
G
Li
Y
Y
D000818:Animals; D017209:Apoptosis; D045744:Cell Line, Tumor; D016147:Genes, Tumor Suppressor; D015316:Genetic Therapy / Q000379:methods*; D051379:Mice; D008819:Mice, Nude; D009303:Nasopharyngeal Neoplasms / Q000628:therapy*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D019253:Proto-Oncogene Proteins c-bcl-2; D014161:Transduction, Genetic; D051028:bcl-2-Associated X Protein
C494827:Bax protein, mouse; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein