Zeolites as new chromatographic carriers for proteins--easy recovery of proteins adsorbed on zeolites by polyethylene glycol.
Zeolites are able to adsorb proteins on their surface and might be suitable as a new type of chromatographic carrier material for proteins and for their conjugates (Matsui et al., Chem. Eur. J. 7 (2001) 1555-1560). Interestingly, maximum adsorption was observed at the isoelectric point (pI) of each protein. The current study was performed to investigate the desorption of proteins from the zeolites at pI. Proteins adsorbed to zeolites could be desorbed at pI by polyethylene glycol (PEG), but not by conventional eluents. The eluted proteins still retained their activities. The zeolite Na-BEA was an especially good composite for desorption by PEG. Using this method for the adsorption and desorption of proteins at pI, we succeeded in separating various proteins. The application of zeolites to biochemistry and biotechnology is also discussed.
Chiku
Hiroyuki
H
Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo, Noda-shi, Chiba-ken 278-8510, Japan.
Matsui
Masayoshi
M
Murakami
Shizuka
S
Kiyozumi
Yoshimichi
Y
Mizukami
Fujio
F
Sakaguchi
Kengo
K
D000327:Adsorption; D002845:Chromatography / Q000379:methods*; D045304:Cytochromes c / Q000302:isolation & purification; D019951:DNA Polymerase beta / Q000302:isolation & purification / Q000378:metabolism; D006454:Hemoglobins / Q000302:isolation & purification; D007526:Isoelectric Point; D009113:Muramidase / Q000302:isolation & purification / Q000378:metabolism; D010047:Ovalbumin / Q000302:isolation & purification; D011092:Polyethylene Glycols / Q000737:chemistry*; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D017641:Zeolites / Q000737:chemistry*
D006454:Hemoglobins; D011506:Proteins; D017641:Zeolites; D011092:Polyethylene Glycols; D010047:Ovalbumin; D045304:Cytochromes c; D019951:DNA Polymerase beta; D009113:Muramidase