Locations and morphologies of sympathetically correlated neurons in the T(10) spinal segment of the rat.
We precisely localized and morphologically characterized sympathetically correlated neurons in the acutely transected spinal cord of the rat. We have shown that these neurons are likely members of the spinal networks that generate sympathetic activity after spinal cord transection. In humans with injured spinal cords, these networks are responsible for hypertensive crises that occur in response to ordinarily innocuous stimuli. We recorded from neurons in the dorsal horn of the T(10) spinal segment of anesthetized rats after acute spinal cord transection at C(2). Neurons with activities closely correlated to renal sympathetic nerve activity (RSNA) were considered to be putative components of spinal sympathetic systems. These neurons had receptive fields on the left flank and abdomen. After characterizing their ongoing activities, receptive fields, and degrees of correlation with RSNA, we juxtacellularly labeled neurons with biotinamide and subsequently reconstructed their somas and dendrites histologically. Confirming our earlier studies, sympathetically correlated neurons were found in dorsal horn laminae III, IV, and V. For the first time, we also identified sympathetically correlated neurons in laminae I and II. The dendrites of all sympathetically correlated neurons projected to multiple lamina. By virtue of the positions of their somas and the broad projections of their dendrites, we concluded that sympathetically correlated neurons may receive direct input both from supraspinal systems and from nociceptive and non-nociceptive primary afferents.
Tang
Xiaorui
X
Department of Biomedical Engineering, The Johns Hopkins School of Medicine, 606 Traylor Building, 720 Rutland Avenue, 21205, Baltimore, MD, USA.
Neckel
Nathan D
ND
Schramm
Lawrence P
LP
D000818:Animals; D020211:Autonomic Dysreflexia / Q000503:physiopathology; D048429:Cell Size; D003712:Dendrites; D004594:Electrophysiology; D007395:Interneurons / Q000502:physiology / Q000648:ultrastructure*; D008297:Male; D009434:Neural Pathways; D020671:Posterior Horn Cells / Q000502:physiology / Q000648:ultrastructure*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000503:physiopathology; D013564:Sympathetic Nervous System / Q000166:cytology* / Q000502:physiology*