title: The effect on shear bond strength of rewetting dry dentin with two desensitizers.
abstract: The difficulty related to achieving a balance between wet and dry dentin makes the dentin bonding technique extremely sensitive. This study evaluated the effect of rewetting dried dentin with two commercial desensitizing agents (Protect and HurriSeal) on the dentin shear bond strength of three total-etch dentin bonding agents (Syntac Single-Component, OptiBond Solo Plus and Prime & Bond NT) and compared both to applying these same bonding agents to moist dentin and dry dentin. Each bonding agent was paired with an appropriate resin composite from the same manufacturer (Table 1). Recently extracted, formalin-treated caries-free human molars were used. The occlusal surface of each tooth was ground to create a flat dentin surface. Then, each tooth was mounted in acrylic. Twelve groups (n = 15) were prepared: 1) Syntac Single-Component with Heliomolar resin composite (SSC/H) to moist dentin; 2) SSC/H to dry dentin; 3) SSC/H to dried dentin rewet with Protect; 4) SSC/H to dried dentin rewet with HurriSeal; 5) OptiBond Solo Plus with Point 4 resin composite (OBS+/P4) to moist dentin; 6) OBS+/P4 to dry dentin; 7) OBS+/P4 to dried dentin rewet with Protect; 8) OBS+/P4 to dried dentin rewet with HurriSeal; 9) Prime & Bond NT with TPH Spectrum resin composite (PBNT/TPH) to moist dentin; 10) PBNT/TPH to dry dentin; 11) PBNT/THP to dried dentin rewet with Protect and 12) PBNT/TPH to dried dentin rewet with HurriSeal. Groups 1, 5 and 9 were placed according to manufacturers' instructions (moist dentin) as control groups. All the other groups received a 15-second air blast after etching and prior to applying the one bottle adhesive or desensitizer and one bottle adhesive. Resin composite cylinders [4 mm in diameter and 2 mm in height] were then placed. The specimens were stored in distilled water at 37 degrees C for 24 hours prior to thermocycling 2,500 times (at 8 degrees C and 48 degrees C). Shear bond strengths (SBSs) were measured one week after fabrication by using a circular knife-edge and crosshead speeds of 0.5 mm/minute. Failure modes of debonded specimens were determined under a stereomicroscope (30x). Failed specimens with the low and high shear bond strengths in each group were evaluated under a low vacuum Scanning Electron Microscope (SEM-LV). One-way ANOVA and Tukey's tests were used to compare the different conditions for each bonding system. In the Syntac Single-Component bonding agent groups, there was no significant difference in shear bond strength between the control (15.73 MPa), dry (18.11 MPa) and HurriSeal (16.18 MPa) specimens. Protect specimens showed significantly lower shear bond strength (6.39 MPa). In the OptiBond Solo Plus bonding agent groups, there was no significant difference between the control (20.79 MPa) and the HurriSeal (21.29 MPa) groups, and both groups had significantly greater bonds than the dry (14.13MPa) and Protect (9.57 MPa) groups. In the Prime & Bond NT bonding agent groups, the shear bond strength of the HurriSeal group (20.73 MPa) was significantly higher than the other groups: control (8.05 MPa), dry (5.73 MPa) and Protect (5.45 MPa).
authors.affiliation: Department of Restorative Dental Science, King Saud University, College of Dentistry, Saudi Arabia. mqahtani@mail.com
authors.forename: Mohammed Q
authors.initials: MQ
authors.lastname: Al Qahtani
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: Platt
authors.forename: B Keith
authors.initials: BK
authors.lastname: Moore
authors.forename: Michael A
authors.initials: MA
authors.lastname: Cochran
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000704:Analysis of Variance; D001548:Benzalkonium Compounds / Q000737:chemistry; D003188:Composite Resins / Q000737:chemistry; D001840:Dental Bonding; D003764:Dental Materials / Q000737:chemistry*; D003804:Dentin / Q000503:physiopathology / Q000648:ultrastructure*; D003807:Dentin Sensitivity / Q000517:prevention & control*; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D006801:Humans; D008689:Methacrylates / Q000737:chemistry; D010070:Oxalates / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000737:chemistry; D011140:Polyurethanes / Q000737:chemistry; D011188:Potassium / Q000737:chemistry; D019279:Resin Cements / Q000737:chemistry; D012969:Sodium Fluoride / Q000737:chemistry; D013314:Stress, Mechanical; D013499:Surface Properties
chemical_list: D000180:Acrylic Resins; D001548:Benzalkonium Compounds; C029012:Composite Dental Resin; D003188:Composite Resins; D003764:Dental Materials; D017220:Dentin-Bonding Agents; C513708:Heliomolar; C403445:Hurriseal; D008689:Methacrylates; C451600:OptiBond Solo Plus; D010070:Oxalates; D011109:Polymethacrylic Acids; D011140:Polyurethanes; C425843:Prime and Bond NT; C090892:Protect Dentin Desensitizer; D019279:Resin Cements; C420128:Spectrum composite resin; C078484:Syntac dentine adhesive; C451741:point 4 composite resin; D012969:Sodium Fluoride; D011188:Potassium