title: Marginal microleakage of two fissure sealants: a comparative study.
abstract: OBJECTIVE
  An in vitro study was conducted to evaluate enamel marginal microleakage following acid etching of two fissure sealants of established clinical efficacy. The purpose of this study was to assess sealant leakage following etching in combination with the use of latest-generation adhesives.
  
  
  METHODS
  Clinicians removed 22 human premolars without caries for orthodontic reasons, stored them in distilled water, and sealed the occlusal surface. They used sealants Concise (3M) and Dyract seal (Dentsply DeTrey). They randomly distributed the premolars into two groups (N = 11) according to sealant used. The materials were manipulated according to the instructions of the manufacturer, and no invasive enamel preparing technique was used (enameloplasty). Staining was carried out with 2% fuchsin. The results were evaluated by the Mann-Whitney nonparametric test for the comparison of independent data samples.
  
  
  RESULTS
  Concise sealant was seen to produce significantly greater marginal leakage than Dyract seal (P=.02).
  
  
  CONCLUSIONS
  The application of an adhesive layer beneath the sealant afforded less microleakage than when no adhesive was used. Nevertheless despite the possibility of combining adhesives and sealant resins, with good bonding between both components over the entire fissure interface, microleakage was still observed, regardless of the material used. This suggests that presently there is no material is able to secure total sealing of the space between the material used and the dental tissue.
authors.affiliation: University of Murcia Dental School, Spain. leonorpe@um.es
authors.forename: Leonor
authors.initials: L
authors.lastname: Pérez-Lajarín
authors.forename: Olga
authors.initials: O
authors.lastname: Cortés-Lillo
authors.forename: Carlos
authors.initials: C
authors.lastname: García-Ballesta
authors.forename: Antonio
authors.initials: A
authors.lastname: Cózar-Hidalgo
mesh_terms: D000134:Acid Etching, Dental; D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry; D020770:Compomers / Q000737:chemistry; D001840:Dental Bonding; D003743:Dental Enamel / Q000648:ultrastructure; D003763:Dental Leakage / Q000145:classification*; D017220:Dentin-Bonding Agents / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D010895:Pit and Fissure Sealants / Q000737:chemistry*; D012394:Rosaniline Dyes; D018709:Statistics, Nonparametric
chemical_list: D020770:Compomers; D017220:Dentin-Bonding Agents; C088486:Dyract; D010895:Pit and Fissure Sealants; D012394:Rosaniline Dyes; D017438:Bisphenol A-Glycidyl Methacrylate