title: Establishment of a prostatic small-cell carcinoma cell line (SO-MI).
abstract: BACKGROUND
  Prostatic small-cell carcinoma is an extremely rare, highly aggressive disease. We established a cell line from this tumor.
  
  
  METHODS
  Tumor tissue obtained from a 24-year-old Japanese man was used to establish the cell line. Cultured cells and tumors transplanted into nude mice were characterized by histologic, immunohistologic, immunocytologic, and molecular biologic methods.
  
  
  RESULTS
  An immortal culture cell line (SO-MI) was successfully established. SO-MI cells adhered weakly to plastic surfaces in vitro, showing a 52- to 72-hr doubling time. SO-MI cells were heterotopically and orthotopically transplantable in nude mice. The cells were immunoreactive for NSE, chromogranin A, and NCAM, but not for ACTH, calcitonin, serotonin, gastrin, insulin, glucagons, LCA, EMA, PAP, PSA, androgen receptor, and p53. SO-MI cells secreted NSE in vitro and in vivo. SO-MI cells at passage 30 contained 50-59 chromosomes with a modal number of 55. PCR suggested that the p53 gene was deleted in SO-MI cells. RT-PCR detected no mRNA encoding androgen receptor in these cells.
  
  
  CONCLUSIONS
  SO-MI cells retain the neuroendocrine nature of the original tumor, and should be useful in studying possible etiologies and new treatments.
authors.affiliation: The Division of Urology, Department of Organs Therapeutics, Faculty of Medicine, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan. okada@med.kobe-u.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Okada
authors.forename: Toshiro
authors.initials: T
authors.lastname: Shirakawa
authors.forename: Hideaki
authors.initials: H
authors.lastname: Miyake
authors.forename: Akinobu
authors.initials: A
authors.lastname: Gotoh
authors.forename: Masato
authors.initials: M
authors.lastname: Fujisawa
authors.forename: Soichi
authors.initials: S
authors.lastname: Arakawa
authors.forename: Sadao
authors.initials: S
authors.lastname: Kamidono
mesh_terms: D000328:Adult; D000818:Animals; D018288:Carcinoma, Small Cell / Q000473:pathology*; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D004273:DNA, Neoplasm; D016158:Genes, p53; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008819:Mice, Nude; D011471:Prostatic Neoplasms / Q000473:pathology*; D012333:RNA, Messenger; D011944:Receptors, Androgen / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
chemical_list: D004273:DNA, Neoplasm; D012333:RNA, Messenger; D011944:Receptors, Androgen