[Determination of quercetin in the Herba Euphorbiae Humifusae].
OBJECTIVE
To develop an HPLC method for determining quercetin in Herba Euphorbiae Humifusae.


METHODS
ODS column was used with acetonitrile: 0.4% phosphoric acid (40:60) as mobile phase. The detection wavelength was at 370 nm and the flow rate was 1.0 mL.min-1.


RESULTS
The linearity was obtained over the range of 0.0364-0.1274 microgram (r = 0.9999). The average recovery was 100.71% with relative standard deviation of 2.45%.


CONCLUSIONS
This method can be used for quality control of Herba Euphorbiae Humifusae. It is simple, rapid and reliable.
Xing
Jun-bo
JB
Institute for Drug and Instrument Control Health Dept GLD of PLA, Beijing 100071, China. junboxing@sohu.com
Shui
Cai-hong
CH
Liu
Yun
Y
D002851:Chromatography, High Pressure Liquid; D028481:Euphorbia / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D011794:Quercetin / Q000032:analysis*
D011794:Quercetin