[Antagonistic effect of aqueous extract of detoxified cottonseeds on corticosterone-induced lesion in cultured PC12 cells].
OBJECTIVE
To study the possible mechanism of aqueous extract of detoxified cottonseeds (CTN-W).


RESULTS
CTN-W 0.01, 0.03, 0.10, 0.30 mg.mL-1 was incubated directly with the synaptic membrane extracted from the cerebral cortex in rats, and adenylyl cyclase (AC) activity was detected by using radio-immunoassay.


RESULTS
Showed that CTN-W could activate AC in a dose-dependend manner. After incubation with PC12 cells in the presence of corticosterone 2 x 10(-4)mol.L-1 for 48 h, CTN-W 0.08, 0.4, 2 mg.mL-1 protected PC12 cells from the lesion induced by corticosterone.


CONCLUSIONS
Antidepressant and anxiolytic effects of CTN-W are related with the activation of AC-cAMP pathway in signal transduction system, thus protecting neurons from the lesion. These two aspects maybe partly form the mechanism of CTN-W's action.
Li
Yun-feng
YF
Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China.
Yang
Ming
M
Zhao
Yi-min
YM
Luan
Xin-hui
XH
Luo
Zhi-pu
ZP
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D014151:Anti-Anxiety Agents / Q000494:pharmacology; D000928:Antidepressive Agents / Q000494:pharmacology; D003345:Corticosterone / Q000037:antagonists & inhibitors; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D003368:Gossypium / Q000737:chemistry*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016716:PC12 Cells / Q000187:drug effects; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry; D013570:Synaptic Membranes / Q000201:enzymology
D014151:Anti-Anxiety Agents; D000928:Antidepressive Agents; D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D000262:Adenylyl Cyclases; D003345:Corticosterone