Lack of interleukin-10 leads to intestinal inflammation, independent of the time at which luminal microbial colonization occurs.
Previous studies have demonstrated that the resident bacteria harbored by interleukin (IL)-10 gene-deficient mice initiate an enterocolitis in the neonatal period. The associated intestinal injury is characterized by an increase in the secretion of interferon (IFN)-gamma and tumor necrosis factor (TNF)-alpha, and by a systemic response to endogenous bacterial antigens, supporting the hypothesis that a lack of tolerance may be the initiating cause. Whether bacterial initiation of this enterocolitis would occur in the adult intestine or whether it is only seen in the developing neonatal intestine was not known. Adult (9 weeks of age), axenic, luminally sterile IL-10 gene-deficient mice, which do not spontaneously develop enterocolitis, were inoculated with intestinal microbial flora. These mice rapidly developed intestinal injury and demonstrated elevated levels of IFN-gamma in cecal and colonic tissue. This response precedes a systemic spleen cell response to stimulation by bacterial antigens. Similarly, axenic, IL-10 gene-deficient mice exposed to microflora as neonates experience a comparable intestinal injury and IFN-gamma release before the appearance of IFN-gamma-producing cells in the spleen. Microbial colonization in control mice leads to systemic IL-10 production, but not systemic IFN-gamma production, suggesting that an IL-10-mediated suppression regulates the response in normal control mice but is absent in IL-10 gene-deficient mice. Our results suggest that the point at which intestinal microbial colonization occurs does not significantly influence the severity or specificity of the inflammatory response in IL-10 gene-deficient mice. The lack of tolerance to bacterial antigens appears to result from the absence of IL-10 during bacterial exposure.
Sydora
Beate C
BC
Department of Medicine, Division of Gastroenterology, University of Alberta, Edmonton, Alberta, Canada.
Tavernini
Michele M
MM
Wessler
Andreas
A
Jewell
Lawrence D
LD
Fedorak
Richard N
RN
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D015169:Colony Count, Microbial; D004797:Enzyme-Linked Immunosorbent Assay; D018836:Inflammation Mediators / Q000032:analysis*; D015212:Inflammatory Bowel Diseases / Q000235:genetics / Q000276:immunology* / Q000382:microbiology*; D007371:Interferon-gamma / Q000032:analysis*; D016753:Interleukin-10 / Q000172:deficiency / Q000235:genetics*; D007413:Intestinal Mucosa / Q000378:metabolism / Q000382:microbiology*; D007420:Intestine, Large / Q000378:metabolism / Q000382:microbiology; D007421:Intestine, Small / Q000378:metabolism / Q000382:microbiology; D051379:Mice; D008815:Mice, Inbred Strains; D023421:Models, Animal; D012016:Reference Values; D012680:Sensitivity and Specificity; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*
D000942:Antigens, Bacterial; D018836:Inflammation Mediators; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007371:Interferon-gamma