[Variation of endogeneous ET, CGRP and NO in myocardial ischemia in rats and the regulatory effect of xinshuping].
OBJECTIVE
To observe the variation of ET and CGRP contents in ischemic heart, and NO level in serum of myocardial damaged rats, and their regulation when with the protection of Xinshuping, a traditional Chinese medicine compound.


METHODS
The models of myocardial ischemia were prepared by subcutaneous injection of isoproterenol or by ligation of coronary artery.


RESULTS
ET content in myocardium was significantly increased (P < 0.01), and CGRP content as well as NO level in serum was not changed obviously in the model induced by isoproterenal. However, NO level in serum of rats treated with Xinshuping (ig bid x 2.5 d) was markedly raised (P < 0.01), neither ET not CGRP contents were affected by it. LDH and CK levels in serum of rats were evidently lowered by Xinshuping treatment. S-T segment's elevation of ECG was significantly inhibited and myocardial infarction size was reduced markedly by Xinshuping treatment in rats subjected to coronary artery ligature.


CONCLUSIONS
ET, CGRP or NO is involved in myocardial infarction caused by isoproterenol. The ischemic damage or dysfunction in different models is obviously protected by Xinshuping. The promotion of NO release from vascular endothelium is probably related with this protective effect.
Liu
Xiao-yan
XY
Department of Pharmacology, School of Pharmaceutical Sciences, Beijing University, Beijing 100083, China.
Wang
Yin-ye
YY
Chen
Shi-zhong
SZ
Li
Chang-ling
CL
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism*; D002316:Cardiotonic Agents / Q000494:pharmacology*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D016232:Endothelins / Q000378:metabolism*; D007545:Isoproterenol; D008297:Male; D009203:Myocardial Infarction / Q000139:chemically induced / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism; D009569:Nitric Oxide / Q000097:blood*; D008517:Phytotherapy; D051381:Rats; D017207:Rats, Sprague-Dawley
D002316:Cardiotonic Agents; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D016232:Endothelins; D009569:Nitric Oxide; D015740:Calcitonin Gene-Related Peptide; D007545:Isoproterenol