Localisation and distribution of Wuchereria bancrofti antigens recognised by antisera from tropical pulmonary eosinophilia and from individuals with intestinal helminths.
Serological analyses of sera from patients with a typical picture of filarial tropical pulmonary eosinophilia (TPE) and sera from patients from a region non-endemic for filariasis harbouring intestinal helminths, as Ascaris lumbricoides and Strongyloids stercoralis, revealed equally high titers of IgG4, usually considered diagnostic for filariasis. Ultrathin sections of adult worms of W. bancrofti embedded in the hydrophilic resin L.R. White were incubated with sera from patients with a typical picture of filarial tropical pulmonary eosinophilia (TPE) and sera from patients of a region that was non-endemic for filarial TPE but endemic for intestinal helminths. Both groups had a similar pattern of labelling, except that the labelling intensity was higher with the sera of patients with filarial TPE. The present study indicates relevant epitopes recognised by sera from TPE-patients and also individuals with intestinal helminths in all tissues of adult and intra-uterine microfilaria of W. bancrofti, instead of being localised in a specific nematode region. These findings suggest that people from areas not endemic for filariasis, but who harbour intestinal helminths, also share antifilarial antibodies in their serum that recognise antigens of adult worms and intrauterine microfilaria of W. bancrofti.
Peixoto
C A
CA
Department of Cellular Pathology and Biology, Research Center 'Aggeu Magalhães, Recife, Brazil. cpeixoto@cpqam.fiocruz.br
Alves
L C
LC
Ferreira Da Silva
L
L
Pires
M L
ML
Rocha
A
A
D000328:Adult; D000368:Aged; D000818:Animals; D000947:Antigens, Helminth / Q000097:blood / Q000276:immunology*; D001196:Ascariasis / Q000097:blood / Q000276:immunology; D017164:Ascaris lumbricoides / Q000276:immunology / Q000302:isolation & purification; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011657:Pulmonary Eosinophilia / Q000097:blood / Q000276:immunology* / Q000473:pathology; D017171:Strongyloides stercoralis / Q000276:immunology / Q000302:isolation & purification; D013322:Strongyloidiasis / Q000097:blood / Q000276:immunology; D014958:Wuchereria bancrofti / Q000276:immunology* / Q000302:isolation & purification / Q000648:ultrastructure
D000947:Antigens, Helminth; C045193:Wuchereria antigen