Error-prone polymerase, DNA polymerase IV, is responsible for transient hypermutation during adaptive mutation in Escherichia coli.
The frequencies of nonselected mutations among adaptive Lac(+) revertants of Escherichia coli strains with and without the error-prone DNA polymerase IV (Pol IV) were compared. This frequency was more than sevenfold lower in the Pol IV-defective strain than in the wild-type strain. Thus, the mutations that occur during hypermutation are due to Pol IV.
Tompkins
Joshua D
JD
Department of Biology, Indiana University, Bloomington, Indiana 47405, USA.
Nelson
Jennifer L
JL
Hazel
Jill C
JC
Leugers
Stacy L
SL
Stumpf
Jeffrey D
JD
Foster
Patricia L
PL
D000222:Adaptation, Physiological; D003470:Culture Media; D019951:DNA Polymerase beta / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D007785:Lactose / Q000378:metabolism; D009154:Mutation
D003470:Culture Media; D029968:Escherichia coli Proteins; D019951:DNA Polymerase beta; D007785:Lactose