[Inhibitory effect of artemin on endotoxin-induced nitric oxide synthesis].
OBJECTIVE
To investigate the effect of Artemin on LPS-induced nitric oxide synthesis in macrophages.


METHODS
1. Nitrite oxide (NO) production of RAW 264.7 cells was induced by LPS or LPS in combination with interferon-gamma (IFN gamma) in the presence or absence of Artemin. The amount of NO in the supernatant of RAW 264.7 cells was detected with Griess reagent. 2. Balb/c mice were injected with Artemin (i.m.) 50 mg.kg-1.d-1 x 3 d, and intraperitoneal macrophages were collected to detect the LPS-induced NO production.


RESULTS
LPS 1.0, 0.2 microgram.ml-1 or IFN-gamma 100u + LPS 1.0, 0.2, 0.04 microgram.ml-1 could induced a large amount of NO synthesis of RAW 264.7 cells. Artemin showed a significant inhibitory effect on LPS or IFN-gamma + LPS-induced NO production in a dose dependent manner. After treatment with Artemin, the response of Balb/c mice to LPS was reduced, which was showed by a decrease in NO production of intraperitoneal macrophages induced by LPS.


CONCLUSIONS
Artemin could reduce LPS-induced production of inflammatory factors resulting in the inhibition of inflammatory effects.
Liang
A H
AH
Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
Xue
B Y
BY
Li
C Y
CY
Wang
J H
JH
Wang
L
L
D000818:Animals; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D007371:Interferon-gamma / Q000037:antagonists & inhibitors; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors; D008264:Macrophages / Q000166:cytology / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009419:Nerve Tissue Proteins / Q000494:pharmacology*; D009569:Nitric Oxide / Q000096:biosynthesis*
C116599:Artn protein, mouse; D008070:Lipopolysaccharides; D009419:Nerve Tissue Proteins; D009569:Nitric Oxide; D007371:Interferon-gamma