title: Carbohydrate and alditol analysis by high-performance anion-exchange chromatography coupled with electrochemical detection at a cobalt-modified electrode.
abstract: A cobalt oxyhydroxide film dispersed on a carbon electrode surface was characterized and proposed as an amperometric sensor for determination of alditols and carbohydrates in flowing streams. Complex mixtures of carbohydrates were separated by anion-exchange chromatography using a moderately alkaline solution as mobile phase. The cobalt modified electrode (GC-Co) was employed under a constant applied potential of 0.5 V (vs Ag/AgCl). Under these experimental conditions the detection limits (S/N=3) for all analyzed electroactive molecules ranged between 0.3 micromol L(-1) and 1.5 micromol L(-1) and the dynamic linear ranges spanned generally three orders of magnitude above the relevant detection limits. Analytical determinations of carbohydrates and alditols in red and white wines, are reported.
authors.affiliation: Dipartimento di Chimica, Universita' degli Studi della Basilicata, Via N. Sauro 85, 85100, Potenza, Italy. casella@unibas.it
authors.forename: Innocenzo G
authors.initials: IG
authors.lastname: Casella
authors.forename: Michela
authors.initials: M
authors.lastname: Contursi
mesh_terms: D002241:Carbohydrates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis; D003035:Cobalt; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013402:Sugar Alcohols / Q000032:analysis*; D014159:Transducers; D014920:Wine / Q000032:analysis* / Q000145:classification
chemical_list: D002241:Carbohydrates; D020099:Coated Materials, Biocompatible; D013402:Sugar Alcohols; D003035:Cobalt