Synthesis of 3'-3'-linked oligonucleotides branched by a pentaerythritol linker and the thermal stabilities of the triplexes with single-stranded DNA or RNA.
Synthesis of 3'-3'-linked oligonucleotides branched by a pentaerythritol linker is described. The branched oligonucleotides were synthesized on a DNA/RNA synthesizer using a controlled pore glass (CPG) with a pentaerythritol linker carrying 4,4'-dimethoxytrityl (DMTr) and levulinyl (Lev) groups. The stability of the triplexes between the branched oligonucleotides and the target single-stranded DNA or RNA was studied by thermal denaturation. The oligonucleotides with the pentaerythritol linker formed thermally stable triplexes with the single-stranded DNA and RNA. Furthermore, the branched oligonucleotides containing 2'-O-methylribonucleosides, especially the oligonucleotide composed of 2'-deoxyribonucleosides and 2'-O-methylribonucleosides, stabilized the triplexes with the single-stranded DNA or RNA. Thus, the branched oligonucleotide containing 2'-O-methylribonucleosides may be a candidate for a novel antisense molecule by the triplex formation.
Ueno
Yoshihito
Y
Department of Biomolecular Science, Faculty of Engineering, Gifu University, Yanagido, Gifu 501-1193, Japan.
Shibata
Aya
A
Matsuda
Akira
A
Kitade
Yukio
Y
D004247:DNA / Q000138:chemical synthesis* / Q000235:genetics; D004277:DNA, Single-Stranded / Q000737:chemistry*; D004355:Drug Stability; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000138:chemical synthesis*; D011409:Propylene Glycols / Q000138:chemical synthesis*; D012313:RNA / Q000138:chemical synthesis* / Q000235:genetics; D013696:Temperature; D013816:Thermodynamics
D004277:DNA, Single-Stranded; D009841:Oligonucleotides; D011409:Propylene Glycols; C051802:triplex DNA; D012313:RNA; D004247:DNA; C008783:pentaerythritol