Low conservation of alternative splicing patterns in the human and mouse genomes.
Alternative splicing has recently emerged as a major mechanism of generating protein diversity in higher eukaryotes. We compared alternative splicing isoforms of 166 pairs of orthologous human and mouse genes. As the mRNA and EST libraries of human and mouse are not complete and thus cannot be compared directly, we instead analyzed whether known cassette exons or alternative splicing sites from one genome are conserved in the other genome. We demonstrate that about half of the analyzed genes have species-specific isoforms, and about a quarter of elementary alternatives are not conserved between the human and mouse genomes. The detailed results of this study are available at www.ig-msk.ru:8005/HMG_paper.
Nurtdinov
Ramil N
RN
Moscow State University, Department of Physics/Biophysics, GSP-2, Leninskie Gory, Moscow 119922, Russia.
Artamonova
Irena I
II
Mironov
Andrei A
AA
Gelfand
Mikhail S
MS
D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D004268:DNA-Binding Proteins / Q000235:genetics; D005091:Exons; D020224:Expressed Sequence Tags; D015894:Genome, Human; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics; D000072260:RNA Splicing Factors; D012333:RNA, Messenger / Q000235:genetics; D016601:RNA-Binding Proteins / Q000235:genetics; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D000096927:AIRE Protein
D004268:DNA-Binding Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D011518:Proto-Oncogene Proteins; D000072260:RNA Splicing Factors; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D000254:Sodium-Potassium-Exchanging ATPase; D014157:Transcription Factors; D000096927:AIRE Protein; C105373:MEN1 protein, human; C490237:NNAT protein, human; C490239:Nnat protein, mouse; C112629:RAD51D protein, human; C491807:Rad51d protein, mouse; C107467:SF1 protein, human; C491514:Sf1 protein, mouse; C491513:Sf1 protein, rat; C416502:FXYD2 protein, human; C498816:Fxyd2 protein, mouse