Analysis of nabumetone in human plasma by HPLC. Application to single dose pharmacokinetic studies.
A simple and sensitive high performance liquid chromatography method for the determination of nabumetone in human plasma is described. The procedure involves liquid-liquid extraction with ethyl acetate and reversed-phase chromatography with fluorimetric detection (excitation 230 nm, emission 356 nm). The chromatographic conditions and the extraction procedure gave a clean chromatogram for the compound. The limit of quantitation was established as 0.313 ng/ml and the calibration curve was linear up to 20 ng/ml. The within-day and between-day relative standard deviations were less than 10% and the accuracy of the assay was in the range of 99-104%. The suitability of the method is shown for pharmacokinetic studies.
Kobylińska
Kamila
K
Department of Pharmacology, Pharmaceutical Research Institute, 8 Rydygiera Street, 01-793 Warsaw, Poland. kkobylin@ifarm.waw.pl
Barlińska
Małgorzata
M
Kobylińska
Maria
M
D002074:Butanones / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002986:Clinical Trials as Topic / Q000379:methods; D006801:Humans; D008297:Male; D000077430:Nabumetone; D015203:Reproducibility of Results
D002074:Butanones; D000077430:Nabumetone