title: Hepatic accumulation and effects of microcystin-LR on juvenile goldfish Carassius auratus L.
abstract: After intraperitoneal injection of microcystin-LR (MC-LR) (125 microg kg(-1) body wt.), the concentration of MC-LR in the liver of juvenile goldfish Carassius auratus (30 g body wt.) was assayed by a modified protein phosphatase inhibition method. A temporary accumulation occurred from 3 to 48 h post-injection, followed by a significant decrease between 48 and 96 h. Under our experimental conditions, contamination by MC-LR did not change ionic homeostasis, as attested by blood osmolality values and gill Na(+)/K(+) ATPase activity. Light microscopy observations revealed lesions and cellular necrosis progression, which was concomitant with an increase in enzyme activity of plasma aspartate aminotransferase (AspAT), alanine aminotransferase (AlaAT) and L-lactate dehydrogenase (LDH) and with a decrease of hepatic glutathione-S-transferase (GST) activity. Structural alterations and enzymatic activity modifications became significant within 24 h post-injection. Recovery of hepatocytes on day 21 after MC-LR injection was evident, together with a decrease in the MC-LR equivalent content of the liver.
authors.affiliation: Unité de Recherche en Biologie des Organismes, Facultés Universitaires Notre-Dame de la Paix, rue de Bruxelles, 61, B-5000 Namur, Belgium. christelle.malbrouck@fundp.ac.be
authors.forename: Christelle
authors.initials: C
authors.lastname: Malbrouck
authors.forename: Gérard
authors.initials: G
authors.lastname: Trausch
authors.forename: Pierre
authors.initials: P
authors.lastname: Devos
authors.forename: Patrick
authors.initials: P
authors.lastname: Kestemont
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001427:Bacterial Toxins / Q000008:administration & dosage / Q000378:metabolism / Q000633:toxicity*; D015415:Biomarkers / Q000032:analysis; D005880:Gills / Q000187:drug effects / Q000201:enzymology; D005982:Glutathione Transferase / Q000378:metabolism; D006054:Goldfish / Q000378:metabolism*; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007274:Injections, Intraperitoneal; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008387:Marine Toxins; D052998:Microcystins; D009994:Osmolar Concentration; D010456:Peptides, Cyclic / Q000008:administration & dosage / Q000378:metabolism / Q000633:toxicity*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
chemical_list: D001427:Bacterial Toxins; D015415:Biomarkers; D008387:Marine Toxins; D052998:Microcystins; D010456:Peptides, Cyclic; D007770:L-Lactate Dehydrogenase; D005982:Glutathione Transferase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000254:Sodium-Potassium-Exchanging ATPase; C057862:cyanoginosin LR