title: Effect of anatomical distribution of mast cells on their defense function against bacterial infections: demonstration using partially mast cell-deficient tg/tg mice.
abstract: Mast cells were depleted in the peritoneal cavity of WBB6F1-tg/tg mice that did not express a transcription factor, MITF. When acute bacterial peritonitis was induced in WBB6F1-+/+, WBB6F1-W/Wv, and WBB6F1-tg/tg mice, the proportion of surviving WBB6F1-+/+ mice was significantly higher than that of surviving WBB6F1-W/Wv or WBB6F1-tg/tg mice. The poor survival of WBB6F1-W/Wv and WBB6F1-tg/tg mice was attributed to the deficient influx of neutrophils into the peritoneal cavity. The injection of cultured mast cells (CMCs) derived from WBB6F1-+/+ mice normalized the neutrophil influx and reduced survival rate in WBB6F1-W/Wv mice, but not in WBB6F1-tg/tg mice. This was not attributable to a defect of neutrophils because injection of TNF-alpha increased the neutrophil influx and survival rate in both WBB6F1-W/Wv and WBB6F1-tg/tg mice. Although WBB6F1-+/+ CMCs injection normalized the number of mast cells in both the peritoneal cavity and mesentery of WBB6F1-W/Wv mice, it normalized the number of mast cells only in the peritoneal cavity of WBB6F1-tg/tg mice. Mast cells within the mesentery or mast cells in the vicinity of blood vessels appeared to play an important role against the acute bacterial peritonitis. WBB6F1-tg/tg mice may be useful for studying the effect of anatomical distribution of mast cells on their antiseptic function.
authors.affiliation: Department of Pathology, Medical School/Graduate School of Frontier Bioscience, Osaka University, Yamada-oka, 2-2, Suita, Osaka 565-0871, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Jippo
authors.forename: Eiichi
authors.initials: E
authors.lastname: Morii
authors.forename: Akihiko
authors.initials: A
authors.lastname: Ito
authors.forename: Yukihiko
authors.initials: Y
authors.lastname: Kitamura
mesh_terms: D000483:Alleles; D000818:Animals; D001424:Bacterial Infections / Q000276:immunology* / Q000473:pathology*; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000172:deficiency / Q000235:genetics; D008407:Mast Cells / Q000276:immunology* / Q000473:pathology* / Q000637:transplantation; D051379:Mice; D008817:Mice, Mutant Strains; D008822:Mice, Transgenic; D051739:Microphthalmia-Associated Transcription Factor; D009154:Mutation; D010529:Peritoneal Cavity / Q000473:pathology; D010538:Peritonitis / Q000276:immunology* / Q000473:pathology*; D014157:Transcription Factors / Q000172:deficiency / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D051739:Microphthalmia-Associated Transcription Factor; C497235:Mitf protein, mouse; D014157:Transcription Factors