title: Sol-gel-derived titanium oxide/copolymer composite based glucose biosensor.
abstract: A new type of sol-gel-derived titanium oxide/copolymer composite material was developed and used for the construction of glucose biosensor. The composite material merged the best properties of the inorganic species, titanium oxide and the organic copolymer, poly(vinyl alcohol) grafting 4-vinylpyridine (PVA-g-PVP). The glucose oxidase entrapped in the composite matrix retained its bioactivity. Morphologies of the composite-modified electrode and the enzyme electrode were characterized with a scanning electron microscope. The dependence of the current responses on enzyme-loading and pH was studied. The response time of the biosensor was < 20 s and the linear range was up to 9 microM with a sensitivity of 405 nA/microM. The biosensor was stable for at least 1 month. In addition, the tetrathiafulvalene-mediated enzyme electrode was constructed for the decrease of detection potential and the effect of three common physiological sources that might interfere was also investigated.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People's Republic of China.
authors.forename: Xu
authors.initials: X
authors.lastname: Chen
authors.forename: Shaojun
authors.initials: S
authors.lastname: Dong
mesh_terms: D000327:Adsorption; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005947:Glucose / Q000032:analysis* / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry*; D008420:Manufactured Materials; D044367:Phase Transition; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D011725:Pyridines / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D011725:Pyridines; C414959:poly(vinyl alcohol) grafting 4-vinylpyridine; C009495:titanium dioxide; D011142:Polyvinyl Alcohol; D014025:Titanium; D005949:Glucose Oxidase; D005947:Glucose