title: Crystallization and preliminary X-ray analysis of a novel unsaturated glucuronyl hydrolase from Bacillus sp. GL1.
abstract: Unsaturated glucuronyl hydrolase from Bacillus sp. GL1 catalyzes the hydrolytic release of unsaturated glucuronic acids from oligosaccharides produced by the reactions of polysaccharide lyases such as gellan, xanthan, hyaluronate and chondroitin lyases. The enzyme was crystallized at 293 K from a droplet containing 56% MPD, 0.1 M NaCl, 0.1 M glycine-NaOH pH 8.2 and 0.1 M dithiothreitol using the vapour-diffusion method. The crystals were hexagonal and belonged to space group P6(1)22 or P6(5)22, with unit-cell parameters a = b = 102.8, c = 223.4 A. Diffraction data to 2.4 A were collected from a single crystal.
authors.affiliation: Division of Food and Biological Science, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan.
authors.forename: Shigetarou
authors.initials: S
authors.lastname: Mori
authors.forename: Sae
authors.initials: S
authors.lastname: Akao
authors.forename: Osamu
authors.initials: O
authors.lastname: Miyake
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Nankai
authors.forename: Wataru
authors.initials: W
authors.lastname: Hashimoto
authors.forename: Bunzo
authors.initials: B
authors.lastname: Mikami
authors.forename: Kousaku
authors.initials: K
authors.lastname: Murata
mesh_terms: D001407:Bacillus / Q000201:enzymology* / Q000235:genetics; D002240:Carbohydrate Sequence; D003460:Crystallization; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000737:chemistry*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D009844:Oligosaccharides; D011994:Recombinant Proteins; D006026:Glycoside Hydrolases; C120983:unsaturated glucuronyl hydrolase