Large-volume sample stacking in nonaqueous capillary electrophoresis using alcoholic run buffers.
A straightforward method for stacking an extremely large volume of a sample solution containing weakly acidic organic compounds was developed. By using alcohols such as methanol, ethanol, and 1-propanol as run buffer solvents, the electroosmotic flow was suppressed, so that analyte anions could proceed to the outlet vial against the electroosmotic flow under a reverse voltage. This effect made large-volume sample stacking possible for large anions using a bare, fused-silica capillary without intermediate polarity switching. The detection limits were in the low nanomolar range with conventional UV absorbance detection. The applicability of the technique to other organic solvents was also tested.
Jang
Jaeho
J
School of Chemistry, Seoul National University, Seoul, Korea.
Koo
Yoon-Mo
YM
Chung
Doo Soo
DS
D000438:Alcohols / Q000737:chemistry*; D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods*; D009930:Organic Chemicals / Q000032:analysis; D012212:Rheology; D012997:Solvents
D000438:Alcohols; D002021:Buffers; D009930:Organic Chemicals; D012997:Solvents