[The electron microscopic study of AchR and ultrastructure of neuromuscular junction in recurrent laryngeal nerve reinnervation].
OBJECTIVE
The purpose of this study was to observe the distribution of Acetylcholine receptor (AchR) and ultrastructural changes of the neuromuscular junction after laryngeal reinnervation.


METHODS
alpha-bungarotoxin-horseradish peroxidase (alpha-BT-HRP) conjugate electron microscopic immunocytochemical methods and computer analysis of intensity of immunocytochemical reaction were applied to observe the distribution and relative quantity of junctional N-AchR.


RESULTS
The results showed that the synaptic nerve terminals and presynaptic membrane were segmentally disrupted and almost disappeared completely and were replaced by Schwann's cells at 3 weeks after neurorrhaphy. At 6 weeks, some of the regenerating axons grew to the neuromuscular junctions and all the muscle fibers were reinnervated at 12 weeks. But the original postsynaptic membranes were only partially in contact with the regenerated nerve terminals. These conditions persisted until 18 weeks after neurorrhaphy, when remyelination was found in the vicinity of the neuromuscular junctions. The N-AchR reaction product on the postsynaptic membrane were increased greatly about 1.28 times more than the normal at 3 weeks after neurorrhaphy. The reactive products decreased greatly which was only 0.47% of that in the normal at 6 weeks. It remained low until 12 weeks after neurorrhaphy, which was 0.72% of the normal side. It increased obviously to 0.83 times of normal sides at 18 weeks and didn't changed there after.


CONCLUSIONS
The state of reinnervation determines the quantity of neuromuscular junctional N-AchR.
Zheng
H
H
Department of Otorhinolaryngology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China. zhenghl@smmu.edu.cn
Zhou
S
S
Yan
Y
Y
Li
Z
Z
Zhang
S
S
Tao
Y
Y
Zhou
R
R
Lu
Y
Y
Zhou
Y
Y
D000818:Animals; D004285:Dogs; D016253:Microscopy, Immunoelectron; D009416:Nerve Regeneration; D009469:Neuromuscular Junction / Q000378:metabolism* / Q000648:ultrastructure; D011950:Receptors, Cholinergic / Q000378:metabolism* / Q000648:ultrastructure; D012009:Recurrent Laryngeal Nerve / Q000502:physiology*; D061226:Recurrent Laryngeal Nerve Injuries
D011950:Receptors, Cholinergic