Classification of oral streptococci by two-dimensional gel electrophoresis with direct activity stain for glycosyltransferases.
Thirty eight strains of oral streptococci were divided into six types by two-dimensional gel electrophoresis (2-DE) followed by glycosyltransferase (GLT) activity stain: Type 1, Streptococcus mutans; Type 2, Streptococcus rattus; Type 3, Streptococcus sobrinus and Streptococcus downei; Type 4, Streptococcus cricetus; Type 5, Streptococcus salivarius; and Type 6, Streptococcus sanguis, Streptococcus oralis and Streptococcus gordonii. In Types 1, 2 and 5, two glucosyltransferases synthesizing water-insoluble (GTF-I) and water-soluble glucans (GTF-S) and a fructosyltransferase (FTF) were detected. In Types 3 and 4, GTF-I and two GTF-Ss were detected. Only one GTF-S was detected in Type 6. The 2-DE patterns for these six types were clearly distinguished from each other based on the kind, number and location of GLTs in gel.
Ando
T
T
Department of Oral and Maxillofacial Surgery, National Defense Medical College, Saitama, Japan.
Tsumori
H
H
Shimamura
A
A
Sato
Y
Y
Mukasa
H
H
D015373:Bacterial Typing Techniques; D015180:Electrophoresis, Gel, Two-Dimensional; D005936:Glucans / Q000096:biosynthesis; D005964:Glucosyltransferases / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D009055:Mouth / Q000382:microbiology*; D013291:Streptococcus / Q000145:classification* / Q000201:enzymology*
D005936:Glucans; D005964:Glucosyltransferases