In vitro and in vivo study of ion-implanted collagen for the substrate of small diameter artificial grafts.
Ion implantation into the collagen-coated inner surface of the grafts was performed and evaluated in vitro and in vivo to develop small diameter artificial vascular grafts. He+ ion implanted collagen-coated grafts with a fluence of 1 x 1014 ions/cm2 inhibited platelet adhesion and demonstrated patency for 240 days in an animal study. The platelet adhesion test using platelet rich plasma (PRP) showed antithrombogenicity at the fluence of 1 x 1014 ions/cm2. Washed platelet adhesion test showed thrombus formation at the fluence of 1 x 1014 ions/cm2. The results suggested that plasma protein adsorption onto the ion-implanted collagen significantly improved performance of these synthetic grafts.
Kurotobi
Kimi
K
Faculty of Science, Science University of Tokyo, Tokyo, Japan.
Suzuki
Yoshiaki
Y
Kaibara
Makoto
M
Iwaki
Masaya
M
Nakajima
Hiroshi
H
Suzuki
Hidenori
H
D000818:Animals; D001798:Blood Proteins / Q000494:pharmacology; D001807:Blood Vessel Prosthesis; D002412:Cations / Q000494:pharmacology*; D003094:Collagen / Q000494:pharmacology*; D004285:Dogs; D006083:Graft Occlusion, Vascular / Q000517:prevention & control; D006371:Helium / Q000494:pharmacology*; D008024:Ligands; D010973:Platelet Adhesiveness / Q000187:drug effects*; D011138:Polytetrafluoroethylene / Q000494:pharmacology*; D013927:Thrombosis / Q000517:prevention & control
D001798:Blood Proteins; D002412:Cations; D008024:Ligands; D006371:Helium; D011138:Polytetrafluoroethylene; D003094:Collagen