title: [An observation on long-term influence of middle ear bacterial infection on inner ear function and systemic immune reaction].
abstract: OBJECTIVE
  To understand whether long-term inner ear heat shock response related to heat shock protein(HSP70) caused by middle ear bacterial infection and the potential influence on inner ear function.
  
  
  METHODS
  Sixty BALB/c mice were randomly classified into 6 groups including Klebsiella pneumoniae (KP), Staphylococcus aureus, Bacillus pyocyaneus, Bacillus coli, Bacillus proteus and physiological saline control groups. On 135 days after injection, distortion product otoacoustic emissions(DPOAE) was tested and all the samples were collected, which were examined with light and electronic microscopes. HSP70 related molecule expression in inner ear, nuclear factor (NF) kappa Bp65 characterization in mononuclear cell, anti-KP antibody and anti-membranous labyrinth proteins (MLP) were examined.
  
  
  RESULTS
  No nuclear transfer of NF kappa Bp65 was observed in any animal. Anti-KP antibody was detected in 30% (3/10) of Staphylococcus aureus group, 29% (2/7) of KP group, 33% (3/9) of Bacillus pyocyaneus group and 10% (1/10) of control group. Anti-MLP antibody was created in 20% (2/10) of Staphylococcus aureus group, 20% (2/10) of KP group, one each in Bacillus pyocyaneus group and control group respectively. Double positive antibody against KP and MLP were found in Staphylococcus aureus group and KP group. When analyzed with Western blot, all the positive bands were small molecules including strongest 26,000-30,000, medium degree 38,000-41,000 and weak 46,000-50,000 except for 68,000 in one case. There was only one significant DPOAE amplification decrease at 1,625 Hz (2f1-f2) in left ear of Bacillus pyocyaneus group and right ear of Bacillus proteus group. No abnormal phenomenon was found in inner ear both under light microscope and electronic microscope. No significant expression of HSP70 was observed in inner ears.
  
  
  CONCLUSIONS
  No long-term heat shock response related to HSP70 existed in the inner ear and the immune inner ear damage may be caused by multiplefactors.
authors.affiliation: Bethune International Peace Hospital, Shijiazhuang 050082, China. zouj@126.com
authors.forename: J
authors.initials: J
authors.lastname: Zou
authors.forename: A
authors.initials: A
authors.lastname: Bi
authors.forename: W
authors.initials: W
authors.lastname: Yang
authors.forename: Y
authors.initials: Y
authors.lastname: Zhou
authors.forename: W
authors.initials: W
authors.lastname: Ding
authors.forename: X
authors.initials: X
authors.lastname: Hao
mesh_terms: D000818:Animals; D001424:Bacterial Infections / Q000276:immunology*; D007758:Ear, Inner / Q000276:immunology* / Q000378:metabolism; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D007711:Klebsiella pneumoniae / Q000302:isolation & purification; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010033:Otitis Media / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification; D011897:Random Allocation; D013211:Staphylococcus aureus / Q000302:isolation & purification
chemical_list: D018840:HSP70 Heat-Shock Proteins