title: The validation of an automated liquid chromatography system for the diagnosis of thalassemias and hemoglobinopathies.
abstract: Accurate and precise hemoglobin separation and the quantitation of Hb A2 and Hb F are essential for the diagnosis of the thalassemias and hemoglobinopathies. Presented in this study is the validation of the the Hb A2 assay of the HbGold analyzer, a fully automated liquid chromatography system for hemoglobin separation and quantitation. Variability of Hb A2 quantitation was quite low; the CV's of within-run, between-run and interlaboratory studies were 1.8-3.1%, 3.4-6.0% and 6.8-8.8% respectively. The results of the %Hb A2 quantitated by HbGold analyzer correlated well with those given by the Bio-Rad Variant Hb testing system (r=0.98). The application of the HbGold analyzer for the diagnosis of the thalassemia phenotypes frequently observed in Thailand is considered. In conclusion, the Hb A2 assay of the HbGold analyzer could be used for the quantitation of Hb A2 and Hb F and the presumptive identification of abnormal hemoglobins.
authors.affiliation: National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand.
authors.forename: Somchai
authors.initials: S
authors.lastname: Sangkitporn
authors.forename: Parichat
authors.initials: P
authors.lastname: Pung-amritt
authors.forename: Siripakorn K
authors.initials: SK
authors.lastname: Sangkitporn
authors.forename: Areerat
authors.initials: A
authors.lastname: Sangnoi
authors.forename: Voravarn S
authors.initials: VS
authors.lastname: Tanphaichitr
mesh_terms: D001331:Automation / Q000379:methods / Q000592:standards; D015982:Bias; D016022:Case-Control Studies; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D005319:Fetal Hemoglobin / Q000378:metabolism; D006580:Genetic Carrier Screening / Q000379:methods; D006443:Hemoglobin A2 / Q000378:metabolism; D006446:Hemoglobin E / Q000378:metabolism; D006453:Hemoglobinopathies / Q000097:blood* / Q000175:diagnosis* / Q000453:epidemiology / Q000235:genetics; D006720:Homozygote; D006801:Humans; D015151:Immunoblotting; D016014:Linear Models; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013785:Thailand / Q000453:epidemiology; D013789:Thalassemia / Q000097:blood* / Q000175:diagnosis* / Q000453:epidemiology / Q000235:genetics
chemical_list: D006443:Hemoglobin A2; D006446:Hemoglobin E; D005319:Fetal Hemoglobin