title: Skeletal muscle myosin is the autoantigen for experimental autoimmune myositis.
abstract: Experimental autoimmune myositis (EAM) is a rodent model for human inflammatory muscle disease (IMD). It can be induced by immunization of rodents with skeletal muscle homogenate and adjuvant. The specific myositogenic autoantigen has not been clearly identified although some evidence points to skeletal muscle myosin. In this report we strengthen this evidence, showing that Lewis rats immunized with purified skeletal muscle myosin develop EAM with the same pattern and severity as EAM induced by whole rabbit skeletal muscle homogenate (WRM). Multiple inflammatory lesions are detected histopathologically in the biceps, quadriceps, and gastrocnemius muscles. Myosin-reactive T cells from animals immunized either with myosin or with WRM have similar patterns of antigen-induced proliferation. The results show that myosin, a component of skeletal muscle, is at least one autoantigen in EAM.
authors.affiliation: Department of Neurology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nemoto
authors.forename: Mahendra K
authors.initials: MK
authors.lastname: Bhopale
authors.forename: Cris S
authors.initials: CS
authors.lastname: Constantinescu
authors.forename: Donald
authors.initials: D
authors.lastname: Schotland
authors.forename: Abdolmohamad
authors.initials: A
authors.lastname: Rostami
mesh_terms: D000818:Animals; D001324:Autoantigens / Q000276:immunology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005620:Freund's Adjuvant / Q000276:immunology / Q000494:pharmacology; D007114:Immunization; D008213:Lymphocyte Activation; D018482:Muscle, Skeletal / Q000276:immunology* / Q000473:pathology; D009218:Myosins / Q000276:immunology* / Q000494:pharmacology; D020721:Nervous System Autoimmune Disease, Experimental / Q000276:immunology* / Q000473:pathology; D017285:Polymyositis / Q000276:immunology* / Q000473:pathology; D011817:Rabbits; D051381:Rats; D011917:Rats, Inbred Lew; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D001324:Autoantigens; D005620:Freund's Adjuvant; D009218:Myosins