title: [Inhibitive effect of puerarin on increased NO production by neonatal cardiomyocytes during hypoxia/reoxygenation injury].
abstract: OBJECTIVE
  To investigate the effect of puerarin (Pue) on nitric oxide (NO) produced by neonatal rat cardiomyocytes during hypoxia/reoxygenation injury.
  
  
  METHODS
  NO contents in the culture supernatants sampled from different groups (control, model, and therapeutic groups with 1, 0.1, 0.01 g.L-1 Pue) at different time were assayed with nitrate reductase method.
  
  
  RESULTS
  NO content of model increased after reperfusion (P < 0.01), while it increased sharply at 6 h after reperfusion and kept thereafter. Only at 6 h after reperfusion did Pue in these three doses inhibit NO production (P < 0.01) and kept to the 24 h after reperfusion.
  
  
  CONCLUSIONS
  Pue exerts inhibitive effect only after NO production is enhanced sharply during hypoxia/reperfusion injury in a dose-dependent trend.
authors.affiliation: Tianjin Medical University, Tianjin 300203, China.
authors.forename: Z T
authors.initials: ZT
authors.lastname: Zhu
authors.forename: H Q
authors.initials: HQ
authors.lastname: Li
authors.forename: Y
authors.initials: Y
authors.lastname: Lu
authors.forename: J S
authors.initials: JS
authors.lastname: Lou
authors.forename: Z
authors.initials: Z
authors.lastname: Yao
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015687:Cell Hypoxia; D002478:Cells, Cultured; D005260:Female; D007529:Isoflavones / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D032383:Myocytes, Cardiac / Q000378:metabolism*; D009569:Nitric Oxide / Q000096:biosynthesis*; D010946:Plants, Medicinal / Q000737:chemistry*; D029908:Pueraria / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D007529:Isoflavones; D009569:Nitric Oxide; C033607:puerarin