title: Xenorhabdus nematophila requires an intact iscRSUA-hscBA-fdx operon to colonize Steinernema carpocapsae nematodes.
abstract: An insertion between iscA and hscB of the Xenorhabdus nematophila iscRSUA-hscBA-fdx locus, predicted to encode Fe-S assembly machinery, prevented colonization of Steinernema carpocapsae nematodes. The insertion disrupted cotranscription of iscA and hscB, but did not reduce hscBA expression, suggesting that X. nematophila requires coordinated expression of the isc-hsc-fdx locus for colonization.
authors.affiliation: Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
authors.forename: Eric C
authors.initials: EC
authors.lastname: Martens
authors.forename: Joseph
authors.initials: J
authors.lastname: Gawronski-Salerno
authors.forename: Danielle L
authors.initials: DL
authors.lastname: Vokal
authors.forename: Molly C
authors.initials: MC
authors.lastname: Pellitteri
authors.forename: Megan L
authors.initials: ML
authors.lastname: Menard
authors.forename: Heidi
authors.initials: H
authors.lastname: Goodrich-Blair
mesh_terms: D000818:Animals; D005288:Ferredoxins; D006360:Heat-Shock Proteins; D007501:Iron / Q000378:metabolism; D018832:Molecular Chaperones; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D016366:Open Reading Frames; D009876:Operon / Q000502:physiology*; D017168:Rhabditida / Q000382:microbiology*; D013455:Sulfur / Q000378:metabolism; D014157:Transcription Factors; D020593:Xenorhabdus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
chemical_list: C081714:2Fe-2S ferredoxin; D005288:Ferredoxins; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D014157:Transcription Factors; D013455:Sulfur; D007501:Iron