Chromatographic classification and comparison of commercially available reversed-phase liquid chromatographic columns using principal component analysis.
A total of 135 commercially available alkyl, cyano, phenyl, perfluorinated, polar embedded, enhanced polar selectivity (i.e., polar/hydrophilic endcapped), "Aqua type" and a variety of novel phases including some non-silica based stationary phases have been characterised in terms of their surface coverage, hydrophobic selectivity, shape selectivity, hydrogen bonding capacity and ion-exchange capacity at pH 2.7 and 7.6. Principal component analysis has been used to provide a simple graphical comparison of the differences/similarities between columns in the entire database and differing subsets such as "Aqua type"/enhanced polar selectivity phases. The PCA has been correlated to the phase's ability to analyse a range of hydrophilic bases.
Euerby
Melvin R
MR
AstraZeneca R & D Charnwood, Analytical Development, Pharmaceutical and Analytical R&D-Charnwood/Lund, Bakewell Road, Loughborough, Leicestershire LE11 5RH, UK. mel.euerby@astrazeneca.com
Petersson
Patrik
P
D002853:Chromatography, Liquid / Q000295:instrumentation*; D006863:Hydrogen-Ion Concentration; D012680:Sensitivity and Specificity