Skeletal muscle mitochondrial oxidative capacity and uncoupling protein 3 are differently influenced by semistarvation and refeeding.
We investigated, in skeletal muscle mitochondria isolated from semistarved and refed rats, the relation between the protein expression of uncoupling protein 3 (UCP3) and mitochondrial oxidative capacity, assessed as state 4 and state 3 respiration rates in presence of substrates that are either non-lipids (glutamate, succinate) or lipids (palmitoyl CoA, palmitoylcarnitine). During semistarvation, when whole-body thermogenesis is diminished, state 3 respiration was lower than in fed controls by about 30% independently of substrate types, while state 4 respiration was lower by 20% only during succinate oxidation, but UCP3 was unaltered. After 5 days of refeeding, when thermogenesis is still diminished, neither state 4, state 3 nor UCP3 were lower than in controls. Refeeding on a high-fat diet, which exacerbates the suppression of thermogenesis, resulted in a two-fold elevation in UCP3 but no change in state 4 or state 3 respiration. These results during semistarvation and refeeding, in line with those previously reported for fasting, are not in support of the hypothesis that UCP3 is a mediator of adaptive thermogenesis pertaining to weight regulation, and underscore the need for caution in interpreting parallel changes in UCP3 and mitochondrial oxidative capacity as the reflection of mitochondrial uncoupling by UCP3.
Crescenzo
R
R
Department of Medicine, Division of Physiology, University of Fribourg, Chemin du Musée 5, Switzerland.
Mainieri
D
D
Solinas
G
G
Montani
J P
JP
Seydoux
J
J
Liverini
G
G
Iossa
S
S
Dulloo
A G
AG
D000821:Animal Feed; D000818:Animals; D015153:Blotting, Western; D001835:Body Weight; D002352:Carrier Proteins / Q000378:metabolism*; D005508:Food Deprivation; D018698:Glutamic Acid / Q000737:chemistry; D007473:Ion Channels; D050356:Lipid Metabolism; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption; D010171:Palmitoyl Coenzyme A / Q000737:chemistry; D010172:Palmitoylcarnitine / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D019802:Succinic Acid / Q000737:chemistry; D000071247:Uncoupling Protein 3
D002352:Carrier Proteins; D007473:Ion Channels; D024101:Mitochondrial Proteins; C000604394:Ucp3 protein, rat; D000071247:Uncoupling Protein 3; D010171:Palmitoyl Coenzyme A; D010172:Palmitoylcarnitine; D018698:Glutamic Acid; D019802:Succinic Acid; D010100:Oxygen