A fluorescent high-throughput assay for double-stranded DNA: the RediPlate PicoGreen assay.
The fluorescent PicoGreen reagent for detection and quantitation of double-stranded DNA has been adapted for high-throughput screening: the RediPlate PicoGreen double-stranded DNA assay format. In the RediPlate PicoGreen assay format, the PicoGreen reagent is predistributed and co-dried into either 96- or 384-well microplates with the excipient trehalose. The user resuspends the dried reagents upon adding DNA, and measures the resulting fluorescence after a five minute incubation. Replicate fluorescence measurements on nominally identical wells have less than a 5% coefficient of variation. The assay is linear from 5 to 500 ng/ml DNA in a 200 micro l volume. The RediPlate PicoGreen assay format retains the advantages of the original PicoGreen reagent - sensitivity, speed, and specificity - but in a high-throughput format.
Batchelor
Robert
R
Probes, Inc., 29851 Willow Creek Road, Eugene, OR 97402, USA. rob.batchelor@probes.com
Hagen
David
D
Johnson
Iain
I
Beechem
Joseph
J
D010582:Bacteriophage lambda / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D005470:Fluorometry; D009930:Organic Chemicals; D015203:Reproducibility of Results
D004279:DNA, Viral; D005456:Fluorescent Dyes; D009930:Organic Chemicals; C099476:PicoGreen