title: Understanding human cancer using Drosophila: Tid47, a cytosolic product of the DnaJ-like tumor suppressor gene l2Tid, is a novel molecular partner of patched related to skin cancer.
abstract: Recessive mutations of the Drosophila gene lethal(2)-tumorous imaginal discs (l(2)tid) cause neoplastic growth of the anlagen of the adult organs, the imaginal discs. Here we report that the three proteins encoded by this evolutionarily conserved gene, Tid50, Tid47, and Tid40, identified as members of the DnaJ cochaperone family, are destined for different cellular compartments, build complexes with many proteins in a developmental stage-specific manner, and are likely to be involved in different cellular processes. We show that the cytosolic Tid47 molecule is a novel component of the Hedgehog (Hh)-Patched (Ptc) signaling regulating cell/tissue polarity and spatial patterning during development and is associated with human tumors such as basal cell carcinoma (BCC) and medulloblastoma. We provide functional evidence for its direct in vivo interaction with the Hh-bound Ptc receptor during signal transmission. Because loss of l(2)tid causes neoplastic transformation of Hh-responsive cells, we suggest that Tid47 may at least act as a guardian of the Hh signaling gradient by regulating Ptc homeostasis in the tissue. Finally, we show that the expression of htid-1, the human counterpart of l(2)tid, is altered in human BCCs. We demonstrate that in BCCs loss of htid expression correlates with loss of differentiation capacity of the neoplastic cells similar to that found in the Drosophila tumor model.
authors.affiliation: Institute of Genetics, Laboratory for Comparative Tumor Biology, Johannes Gutenberg University, 55131 Mainz, Germany.
authors.forename: Itziar
authors.initials: I
authors.lastname: Canamasas
authors.forename: Anette
authors.initials: A
authors.lastname: Debes
authors.forename: Pier Giorgio
authors.initials: PG
authors.lastname: Natali
authors.forename: Ursula
authors.initials: U
authors.lastname: Kurzik-Dumke
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D002280:Basal Cell Carcinoma / Q000235:genetics* / Q000473:pathology; D002454:Cell Differentiation; D016764:Cell Polarity; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D002528:Cerebellar Neoplasms / Q000235:genetics / Q000473:pathology; D002874:Chromosome Mapping; D004330:Drosophila / Q000235:genetics* / Q000254:growth & development; D029721:Drosophila Proteins; D019143:Evolution, Molecular; D005260:Female; D018507:Gene Expression Regulation, Developmental; D015972:Gene Expression Regulation, Neoplastic; D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007814:Larva / Q000235:genetics; D008024:Ligands; D008297:Male; D008527:Medulloblastoma / Q000235:genetics / Q000473:pathology; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D024101:Mitochondrial Proteins; D000072079:Patched Receptors; D010641:Phenotype; D017434:Protein Structure, Tertiary; D011956:Receptors, Cell Surface; D012878:Skin Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: C093721:CG5504 protein, Drosophila; D029721:Drosophila Proteins; D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D008024:Ligands; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D000072079:Patched Receptors; D011956:Receptors, Cell Surface; C494638:Tid47 protein, Drosophila