title: Immunocytochemical applications of specific antisera raised against synthetic fragment peptides of mummichog GtH subunits: examining seasonal variations of gonadotrophs (FSH cells and LH cells) in the mummichog and applications to other acanthopterygian fishes.
abstract: Two distinct types of gonadotrophs, FSH (GtH I) cells and LH (GtH II) cells, were immunocytochemically identified from mummichog (Fundulus heteroclitus; Cyprinodontiformes, Acanthopterygii) pituitary using antisera raised against synthetic fragment peptides of FSHbeta and LHbeta. Both cell types were abundant during the spawning period (spring and early summer) and decreased in number during the post-spawning immature period. The number of FSH cells increased again during the early phases of gonadal development (cortical alveoli accumulation in the oocytes and basal spermatogenesis) in early winter, whereas the number of LH cells did not. Only FSH cells were abundant during the latter phases of gonadal development (vitellogenesis and active spermatogenesis) in early spring. These observations suggest that both GtHs have important yet different roles for reproduction in this species. Antisera against the conservative region of the FSHbeta and the LHbeta subunits immunostained FSH cells and LH cells, respectively, also in red seabream (Pagrus major; Perciformes, Acanthopterygii) and small mouth bass (Micropterus dolomieu; Perciformes, Acanthopterygii), suggesting the possibility of their use for other acanthopterygian fishes.
authors.affiliation: National Research Institute of Fisheries Science, Fisheries Research Agency, Fukuura 2-12-4, Kanazawa, Yokohama 236-8648, Japan. aneko@affrc.go.jp
authors.forename: Akio
authors.initials: A
authors.lastname: Shimizu
authors.forename: Hideki
authors.initials: H
authors.lastname: Tanaka
authors.forename: Hirohiko
authors.initials: H
authors.lastname: Kagawa
mesh_terms: D000818:Animals; D001492:Bass / Q000378:metabolism; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism*; D023781:Fundulidae / Q000378:metabolism*; D007106:Immune Sera / Q000276:immunology; D007150:Immunohistochemistry; D007986:Luteinizing Hormone / Q000378:metabolism*; D008297:Male; D010446:Peptide Fragments / Q000276:immunology; D010902:Pituitary Gland / Q000166:cytology* / Q000378:metabolism*; D021541:Sea Bream / Q000378:metabolism; D012621:Seasons; D012726:Sexual Behavior, Animal / Q000502:physiology
chemical_list: D007106:Immune Sera; D010446:Peptide Fragments; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone