title: Simultaneous identification of soyasaponins and isoflavones and quantification of soyasaponin Bb in soy products, using liquid chromatography/electrospray ionization-mass spectrometry.
abstract: A method has been developed for simultaneous identification of soyasaponins and soy isoflavones in soy products, based on liquid chromatography/electrospray ionization-mass spectrometry (LC/ESI-MS). Soy-based nutraceutical products were analyzed by LC/ESI-MS with detection of protonated and sodiated molecular ions, as well as characteristic fragment ions for these compounds. Soy isoflavones were characterized by a strong protonated molecular ion in addition to corresponding [aglycone + H](+) ions. Monitoring the soyasaponin-specific protonated aglycone and dehydrated aglycone ions throughout the chromatogram provided a unique fingerprint for soyasaponin content in the samples. This mass spectrometric fingerprint also allowed immediate classification of the soyasaponin analytes as group A or B soyasaponins, based on the unique masses of aglycone ions observed for each class. Quantification of soyasaponin B(b) in soy-derived materials, based on the use of a purified soyasaponin B(b) standard and a glycyrrhizin internal standard, has been accomplished.
authors.affiliation: Cargill Scientific Resources Center and Health and Food Technologies Division, Cargill Incorporated, P.O. Box 5702, Minneapolis, Minnesota 55440-5702, USA. joseph_dalluge@cargill.com
authors.forename: Joseph J
authors.initials: JJ
authors.lastname: Dalluge
authors.forename: Erik
authors.initials: E
authors.lastname: Eliason
authors.forename: Scott
authors.initials: S
authors.lastname: Frazer
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007529:Isoflavones / Q000032:analysis* / Q000737:chemistry; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D012503:Saponins / Q000032:analysis* / Q000737:chemistry; D013025:Glycine max / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D007529:Isoflavones; D012503:Saponins; C407096:soyasapogenol B; D009828:Oleanolic Acid