[Effect of blocking transforming growth factor beta signalling on culture-activated rat hepatic stellate cells].
OBJECTIVE
To study the effects of blocking transforming growth factor beta (TGF-beta) signalling on culture-activated rat hepatic stellate cells (HSCs).


METHODS
After cultured in plastic dish for two days, HSCs were infected with adenovirus vector AdT beta-ExR or AdLacZ (control) at 10 multiplicity of infection (MOI) and incubated for four days. The expression of type I collagen, alpha-smooth muscle actin (alpha-SMA) and the proliferation of HSCs were analyzed by ELISA, western blot, immunocytochemistry and BrdU uptake respectively.


RESULTS
The expression level of type I collagen in HSCs infected with AdT beta-ExR was 42.99% of that in HSCs infected with AdLacZ (q = 9.100, P < 0.001). The expression of alpha-SMA in HSCs infected with AdTbeta-ExR was also inhibited evidently. But the BrdU uptake in HSCs infected with AdLacZ was 49.24% of that in HSCs infected with AdTbeta-ExR (q = 7.835, P < 0.001).


CONCLUSIONS
The blockade of TGF-beta signalling in cultured rat HSCs can inhibit their activation significantly, but promote their proliferation.
Zhou
Ya-jun
YJ
Department of Biochemistry, Nantong Medical College, Jiangsu Province, Nantong 226001, China.
Yin
Dong-mei
DM
Chen
Hong-shan
HS
Zhu
Hui-xia
HX
Wang
Xin
X
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis / Q000235:genetics; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D008099:Liver / Q000187:drug effects / Q000473:pathology* / Q000502:physiology; D008103:Liver Cirrhosis / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D024042:Collagen Type I; D016212:Transforming Growth Factor beta