[Study on stem-tip tissue culture of the traditional Chinese medicine Chrysanthemum morifolium].
OBJECTIVE
To set up the optimums for the stem-tip tissue culture of Chrysanthemum morifolium cultivated in Anhui Province.


METHODS
Small sections (about 0.5 mm in length) from the stem-tips were isolated and inoculated with different media, and induced to form the whole plantlet formation.


CONCLUSIONS
The MS medium added with 6-BA 2 mg.L-1 + NAA 0.2 mg.L-1 was the optimum medium for the bud sprouting and the inducing rate was over 80% after 40 d cultivation on this modified medium. The MS medium supplemented with 6-BA 2 mg.L-1 and NAA 0.5 mg.L-1 was the optimum medium for the multiplication of the adventitious buds in which the bud multiplication was about 4-7 times higher after 25-30 d cultivation. The plantlet could root well on the MS medium with NAA 0.5 mg.L-1.
Xue
Jian-ping
JP
Department of Biology, Huaibei Coal Industry Teacher's College, Huaibei 235000, Anhui, China.
Zhang
Ai-min
AM
Sheng
Wei
W
Zhao
Feng-lan
FL
D031202:Chrysanthemum / Q000254:growth & development*; D046508:Culture Techniques; D018547:Plant Stems / Q000254:growth & development