[Antioxidant effect of Ganoderma polysaccharide peptide].
OBJECTIVE
To study the antioxidant effect of Ganoderma polysaccharide peptide (GLPP) and its mechanism.


METHODS
Copper was used as oxidant to induce low lipoprotein (LDL) oxidative modification, and alloxan was given i.v. to induce reactive oxygen species (ROS) injury in mice.


RESULTS
GLPP decreased oxidation of LDL and the relative electrophoretic mobility (REM) of oxidative product of LDL. After GLPP was given i.p. for 20 days, the concentration of malondialdehyde(MDA) in serum and heart of mice was decreased. The GSHpx enzyme activity was increased, while the SOD level was decreased. The catalase(CAT) levels were not significantly changed by GLPP.


CONCLUSIONS
GLPP showed antioxidant effect by scavenging ROS or enhancing the enzyme activity of GSHpx in vivo and in vitro.
You
Yu-hong
YH
Department of Pharmacology, School of Basic Medical Sciences, Health Science Center, Peking University, Beijing 100083, China.
Lin
Zhi-bin
ZB
D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000097:blood / Q000378:metabolism; D051379:Mice; D009206:Myocardium / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D010946:Plants, Medicinal / Q000737:chemistry; D011509:Proteoglycans / Q000302:isolation & purification / Q000494:pharmacology*; D011897:Random Allocation; D020940:Reishi / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D008077:Lipoproteins, LDL; D011509:Proteoglycans; C093216:polysaccharide peptide; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase