title: Apolipophorin-III affects the activity of the haemocytes of Galleria mellonella larvae.
abstract: Apolipophorin-III (apoLp-III) impaired the adhesion of plasmatocytes and a granular cell-subpopulation of larval Galleria mellonella to glass slides. The protein bound to haemocytes, limited the responses of the plasmatocytes to Bacillus subtilis and increased the percentage of a subgroup of granular cells with adhering bacteria. The total number of bacteria adhering to all the haemocytes on the slides declined. Injections of apoLp-III slowed bacterial removal from the haemolymph without affecting total haemocyte counts and impaired haemocyte attachment to glass slides. Purified apoLp-III bound to B. subtilis. ApoLp-III in serum bound to bacteria within 5 min, peaked at 15 min and was either shed or dissociated by 60 min. ApoLp-III bound to B. subtilis lowered the adhesion of the bacteria to the haemocytes and slowed the removal of the bacteria from the haemolymph.
authors.affiliation: Department of Natural Resource Sciences, McGill University, 21, 111 Lakeshore Road, Ste. Anne de Bellevue, H9X 3V9, Québec, Canada
authors.forename: Robert J.
authors.initials: RJ
authors.lastname: Zakarian
authors.forename: Gary B.
authors.initials: GB
authors.lastname: Dunphy
authors.forename: Paul J.
authors.initials: PJ
authors.lastname: Albert
authors.forename: Manfred E.
authors.initials: ME
authors.lastname: Rau