[Effects of nitric oxide on ciliary beat frequency in the human nasal mucosa].
OBJECTIVE
To investigate the effect of nitric oxide on ciliary beat frequency in human nasal mucosa.


METHODS
A primary culture model of human nasal mucosa epithelial cells was established and the effect of nitric oxide on ciliary beat frequency was observed by videomicroscopy method. The data were statistically analyzed using t-test.


RESULTS
L-arginine significantly increased ciliary beat frequency from (400 +/- 48) beats/min to (793 +/- 68) beats/min (n = 9, P < 0.001), D-arginine had no similar effect, while NOS blocker L-NAME inhibited the effect of L-arginine.


CONCLUSIONS
Cells cultured by our method could synthesize NOS that made L-arginine produce NO that could increase ciliary beat frequency in the human nasal mucosa.
Wang
F
F
Department of Otorhinolaryngology, General Hospital of PLA, Beijing 100853, China. wangfeng304@sohu.com
Su
Z
Z
Wang
L
L
D001120:Arginine / Q000502:physiology; D002478:Cells, Cultured; D002923:Cilia / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D009297:Nasal Mucosa / Q000473:pathology*; D009298:Nasal Polyps / Q000473:pathology; D009569:Nitric Oxide / Q000502:physiology*; D012852:Sinusitis / Q000473:pathology
D009569:Nitric Oxide; D001120:Arginine