title: Validation and comparison of the use of diuresis cystometry and retrograde filling cystometry at various infusion rates in female Beagle dogs.
abstract: OBJECTIVE
  To compare retrograde filling cystometry at infusion rates of 5, 10, and 20 mL/min with diuresis cystometry for determination of an appropriate infusion rate and to confirm the reproducibility of measurements obtained by urethral pressure profilometry (UPP) and cystometry in female Beagles.
  
  
  METHODS
  Adult female Beagles.
  
  
  METHODS
  Successive UPP and cystometry were performed by use of a water perfusion catheter on dogs anesthetized with propofol. Dogs randomly underwent each of the following at 1-week intervals: retrograde filling cystometry at 5, 10, and 20 mL/min, and diuresis cystometry. The maximum urethral pressure and closure pressure, functional and anatomic profile lengths, threshold pressure, threshold volume, and compliance were measured.
  
  
  RESULTS
  For each UPP variable, significant differences were found among dogs, but no significant differences were found in intra- or interstudy measurements for individual dogs. For retrograde filling cystometry, threshold pressure was not significantly different between a 5 and 10 mL/min infusion rate. Threshold pressure was significantly higher during retrograde filling cystometry at 20 mL/min, compared with 5 and 10 mL/min, and was associated with bladder wall damages. Threshold pressure was significantly lower during diuresis cystometry, compared with retrograde filling cystometries. Threshold volume and compliance were not significantly different among retrograde filling cystometries but were significantly higher during diuresis cystometry.
  
  
  CONCLUSIONS
  Retrograde filling cystometry at 20 mL/min leads to unacceptable sudden increase in threshold bladder pressure. Retrograde filling cystometry at 10 mL/min can be recommended in a clinical setting, shortening the anesthesia time. However, diuresis cystometry approximates physiologic bladder filling most accurately.
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, University of Liège, Bld de Colonster, 20 B44, Sart Tilman, 4000 Liège, Belgium.
authors.forename: Annick J
authors.initials: AJ
authors.lastname: Hamaide
authors.forename: John P
authors.initials: JP
authors.lastname: Verstegen
authors.forename: Frédéric R
authors.initials: FR
authors.lastname: Snaps
authors.forename: Karine
authors.initials: K
authors.lastname: Onclin
authors.forename: Marc H
authors.initials: MH
authors.lastname: Balligand
mesh_terms: D000818:Animals; D003950:Diagnostic Techniques, Urological / Q000592:standards / Q000662:veterinary*; D004231:Diuresis / Q000502:physiology*; D004285:Dogs; D005260:Female; D011312:Pressure; D015203:Reproducibility of Results; D014521:Urethra / Q000502:physiology*; D001743:Urinary Bladder / Q000502:physiology*; D014563:Urodynamics / Q000502:physiology*