Developmental distribution of coxsackie virus and adenovirus receptor localized in the nervous system.
Mouse coxsackie virus and adenovirus receptor (mCAR), which was isolated from the nerve growth cone-enriched fraction of newborn mouse brains, is a member of immunoglobulin-super family, and functions as a homophilic adhesion molecule. We observed the expression of mCAR in embryos to adult tissues by means of immunohistochemical analysis with a peptide antibody. mCAR expression was first detected in the embryonic ectoderm in the uterus on embryonic day 6.5 (E6.5). Then it was strongly expressed in the neuroepithelium of the neural tube, the developing brain and the spinal cord from E8.5 to postnatal day 7 (P7), in the cranial motor nerves from E9.5 to E11.5, and in the optic nerve from E13.5 to P7, which agrees with periods of their respective morphogenetic peaks. This expression of mCAR decreased postnatally and was absent in adult tissues. We found that mCAR occurred in a few proliferating cells of the hippocampal dentate gyrus, the subventricular zone (SVZ) of the lateral ventricles, and the rostral migratory stream (RMS) over P21. These observations demonstrate that mCAR was expressed characteristically in the immature neuroepithelium including progenitor cells or radial cells derived from the neural tube and in immature cells in a selected germinal zone of the mature brain. Based on our findings, we propose that mCAR is involved in migration and fasciculation during a restricted period as an adhesion molecule.
Hotta
Yuko
Y
Department of Molecular Genetics, Genome Science Branch, Center for Bioresource-based Researches, Brain Research Institute, Niigata University, Niigata, Japan.
Honda
Takao
T
Naito
Makoto
M
Kuwano
Ryozo
R
D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D046508:Culture Techniques; D004027:Diencephalon / Q000033:anatomy & histology / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D004475:Ectoderm / Q000378:metabolism; D004622:Embryo, Mammalian / Q000378:metabolism*; D005314:Embryonic and Fetal Development; D004848:Epithelium / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D005123:Eye / Q000033:anatomy & histology / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000502:physiology; D007150:Immunohistochemistry / Q000379:methods; D007381:Intermediate Filament Proteins / Q000378:metabolism; D051379:Mice; D009297:Nasal Mucosa / Q000378:metabolism; D009419:Nerve Tissue Proteins; D064231:Nestin; D016900:Neurofilament Proteins / Q000378:metabolism; D009666:Nose / Q000033:anatomy & histology / Q000196:embryology / Q000254:growth & development; D010446:Peptide Fragments / Q000276:immunology / Q000378:metabolism; D011247:Pregnancy; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011991:Receptors, Virus / Q000378:metabolism*; D013116:Spinal Cord / Q000033:anatomy & histology / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D013687:Telencephalon / Q000033:anatomy & histology / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism
C568772:CLMP protein, mouse; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; D016900:Neurofilament Proteins; D010446:Peptide Fragments; D018809:Proliferating Cell Nuclear Antigen; D011991:Receptors, Virus; D014404:Tubulin; C050934:neurofilament protein 150