[Experimental study on effect of Astragalus extractum on canine isolated kidney during hypothermia perfusion and preservation].
OBJECTIVE
To investigate the effect of Astragalus Extractum on canine isolated kidney during hypothermia perfusion and preservation.


METHODS
Isolated kidneys in the control group were hypothermia perfused and preserved using conventional hypertonic adenine citrate solution (HC-A), and for those in the experimental group, using HC-A plus Astragalus extract instead. The changes of renal tissue construction were observed with light microscopy and electron microscopy. Moreover, the kidney transplantation model of dog was established to determine the changes of biochemical parameters before and after transplantation. Data were analysed synthetically.


RESULTS
The ultrastructural injury in preserved kidney of the experimental group was significantly milder than that in the control group. Parameters determined in the early stage of transplantation showed that the blood creatinine level was significantly lower and the endogenous creatinine clearing value was higher in the experimental group than that in the control group, the difference was significant (P < 0.05).


CONCLUSIONS
When Astragalus extractum is used in preserving kidney with hypothermia perfusion, it shows definite protective effect on the ischemic reperfusion injured kidney.
Yuan
Chun-hui
CH
Department of Organ Transplantation, First Affiliated Hospital, China Medical University, Shenyang 110001.
Liu
Yong-feng
YF
Cheng
Ying
Y
D000818:Animals; D027883:Astragalus Plant / Q000737:chemistry*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004285:Dogs; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D007036:Hypothermia, Induced; D066298:In Vitro Techniques; D007668:Kidney; D016030:Kidney Transplantation; D008297:Male; D015427:Reperfusion Injury / Q000517:prevention & control; D014182:Transplantation, Autologous
D003451:Cryoprotective Agents; D004365:Drugs, Chinese Herbal