Reactive astrocytes express cAMP-response-element-binding protein (CREB) binding protein (CBP) in the central nervous system of transgenic mice expressing a human Cu/Zn superoxide dismutase mutation.
In the present study, we used the superoxide dismutase 1(SODI)(G93A) transgenic mice as an in vivo model of amyotrophic lateral sclerosis (ALS) and performed immunohistochemical studies to investigate the changes of cAMP-response-element-binding protein (CREB) binding protein (CBP) in the central nervous system of transgenic mice. The distribution of CBP-immunoreactive neurons was not different between control and transgenic mice, whereas CBP-immunoreactive astrocytes were found only in transgenic mice. CBP-immunoreactive astrocytes were detected in the spinal cord, brainstem, midbrain and cerebellar nuclei of transgenic mice. The present study provides the first evidence that CBP immunoreactive astrocytes were observed in the central nervous system of transgenic mice, suggesting that reactive astrocytes may play an important role in the pathogenesis and progress of ALS.
Chung
Yoon Hee
YH
Department of Anatomy, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-Gu, Seoul 110-799, South Korea.
Joo
Kyeung Min
KM
Lee
Yun Jung
YJ
Kim
Myeung Ju
MJ
Cha
Choong Ik
CI
D000690:Amyotrophic Lateral Sclerosis / Q000235:genetics; D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D050882:CREB-Binding Protein; D002490:Central Nervous System / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008822:Mice, Transgenic; D009046:Motor Neurons / Q000201:enzymology; D009154:Mutation / Q000235:genetics*; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000235:genetics*; D018384:Oxidative Stress / Q000502:physiology; D013482:Superoxide Dismutase / Q000235:genetics*; D015534:Trans-Activators / Q000096:biosynthesis* / Q000235:genetics*
D009687:Nuclear Proteins; D015534:Trans-Activators; D013482:Superoxide Dismutase; D050882:CREB-Binding Protein; C494363:CREBBP protein, human; C494364:Crebbp protein, mouse