title: Fluorescence assays for high-throughput screening of protein kinases.
abstract: Protein kinases comprise one of the most important group of targets for drug discovery research today. Methods to identify novel kinase inhibitors by high-throughput screening have evolved rapidly in recent years. An important aspect is the availability of fluorescent probes that can be applied in a homogeneous, or mix-and-measure, assay format. Here, we illustrate the application of fluorescence read-out technologies for kinase targets in light of our own experiences in assay development and high-throughput screening.
authors.affiliation: Lead Discovery Unit, N V Organon, The Netherlands. guido.zaman@organon.com
authors.forename: G J R
authors.initials: GJ
authors.lastname: Zaman
authors.forename: A
authors.initials: A
authors.lastname: Garritsen
authors.forename: Th
authors.initials: T
authors.lastname: de Boer
authors.forename: C A A
authors.initials: CA
authors.lastname: van Boeckel
mesh_terms: D000818:Animals; D020650:Combinatorial Chemistry Techniques / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D005470:Fluorometry / Q000379:methods*; D006801:Humans; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000032:analysis* / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases