title: Quality assessment of seven types of fresh-frozen plasma leucoreduced by specific plasma filtration.
abstract: OBJECTIVE
  A study was undertaken to determine plasma quality after specific filtration.
  
  
  METHODS
  Seven types of plasma were tested, after filtration of plasma from filtered or non-filtered whole blood. Leucocyte counting was carried out after a 30-fold concentration of the sample. Twenty-nine parameters (including coagulation testing, proteins, coagulation factors and activation markers) were measured before and after filtration, and after 6 months of storage.
  
  
  RESULTS
  After specific plasma filtration, the average residual leucocyte counts were less than 2250/l. In spite of small statistically significant changes in proteins, coagulation factors and complement activation, this study showed that plasma filtration did not alter plasma quality. After 6 months of storage at -30 degrees C, factor VIII recovery varied between 91 and 109%. Haemostasis parameters and activation markers remained within the normal range.
  
  
  CONCLUSIONS
  Specific plasma filtration reduced the leucocyte number to < 104 leucocytes/l. The quality of plasma was not altered by the additional step of specific plasma filtration.
authors.affiliation: Etablissement Français du Sang, Paris, France. anne.chabanel@efs.sante.fr
authors.forename: A
authors.initials: A
authors.lastname: Chabanel
authors.forename: I
authors.initials: I
authors.lastname: Sensebé
authors.forename: M
authors.initials: M
authors.lastname: Masse
authors.forename: J P
authors.initials: JP
authors.lastname: Maurel
authors.forename: J
authors.initials: J
authors.lastname: Plante
authors.forename: D
authors.initials: D
authors.lastname: Hivet
authors.forename: C
authors.initials: C
authors.lastname: Kannengiesser
authors.forename: C
authors.initials: C
authors.lastname: Naegelen
authors.forename: M
authors.initials: M
authors.lastname: Joussemet
authors.forename: B
authors.initials: B
authors.lastname: Marchesseau
authors.forename: P
authors.initials: P
authors.lastname: Rasongles
authors.forename: F
authors.initials: F
authors.lastname: Proust
authors.forename: C
authors.initials: C
authors.lastname: David
authors.forename: A M
authors.initials: AM
authors.lastname: Montembault
authors.forename: P
authors.initials: P
authors.lastname: Bergeat
mesh_terms: D015415:Biomarkers / Q000097:blood; D001780:Blood Coagulation Tests; D001793:Blood Preservation; D002469:Cell Separation / Q000379:methods; D005169:Factor VIII / Q000032:analysis; D005374:Filtration; D006487:Hemostasis; D006801:Humans; D007958:Leukocyte Count; D007962:Leukocytes; D010949:Plasma; D011785:Quality Assurance, Health Care
chemical_list: D015415:Biomarkers; D005169:Factor VIII