[Experimental study of total nutrient admixture promoting transfection of plasmid DNA mediated with liposomes to colorectal cancer cells].
OBJECTIVE
To study total nutrient admixture (TNA) promoting plasmid DNA transfection mediated with liposomes to colorectal cancer cells.


METHODS
Dispensing varied transfection agents of liposome + DNA plasmid pEGFP-N(1), TNA + liposomes + pEGFP-N(1), TNA + pEGFP-N(1), liposomes merely, and TAN sole. Human colorectal cancer cell LoVo and HR-8348 were treated with the agents respectively. Green fluorescence protein (GFP) gene as a report gene was detected.


RESULTS
GFP was not detected in cancer cells treated with agents of merely liposomes and TAN. Transfection rates of GFP in two groups of cancer cells treated with agent of TNA + liposomes + pEGFP-N(1) were 33%, 38% respectively. With liposome + pEGFP-N(1), the rates of transfection in two cells were 22%, 24% respectively. The expression of GFP was 1% in the two groups of tumor cells treated with TNA + pEGFP-N(1). With agent of TNA + liposomes + pEGFP-N(1), a high transfection rate of GFP gene was obtained. And no negative effect was observed to stabilization of TAN solution.


CONCLUSIONS
TNA may enhance transfection rate of plasmid DNA mediated with liposome, and may be beneficial to the treatment of cancer.
An
Ping
P
Department of General Surgery, Beijing Military Area General Hospital, Beijing 100700, China.
Yu
Bo
B
Li
Shi-yong
SY
Cai
Hui-yun
HY
D015179:Colorectal Neoplasms / Q000628:therapy*; D015316:Genetic Therapy; D006801:Humans; D008081:Liposomes; D018529:Nutritional Support; D010957:Plasmids; D014162:Transfection / Q000379:methods*
D008081:Liposomes