Novel protein vaccine candidates against Group B streptococcal infection identified using alkaline phosphatase fusions.
Using an alkaline phosphatase-based genetic screening method, we identified a number of proteins that are potentially located on the outer surface of Group B streptococcus (Streptococcus agalactiae). In an enzyme-linked immunosorbent assay, antisera raised against two of the proteins, the streptococcal yutD homologue and a subunit of an ABC transporter, recognised clinically important serotypes of Group B streptococcus. In a neonatal rat model, purified IgG from the sera conferred significant levels of protection against a lethal challenge infection. The proteins identified show potential as protein subunit candidates for vaccines against Group B streptococcal disease in neonates.
Hughes
Martin J G
MJ
Microscience Ltd., 545 Eskdale Road, Winnersh Triangle, Berks RG41 5TU, Wokingham, UK
Wilson
Rebecca
R
Moore
Joanne C
JC
Lane
Jonathan D
JD
Dobson
Richard J
RJ
Muckett
Phillip
P
Younes
Zabin
Z
Pribul
Philippa
P
Topping
Andrew
A
Feldman
Robert G
RG
Santangelo
Joseph D
JD
D000469:Alkaline Phosphatase / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines / Q000235:genetics* / Q000494:pharmacology; D003001:Cloning, Molecular; D004926:Escherichia coli; D007074:Immunoglobulin G; D011817:Rabbits; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D013290:Streptococcal Infections / Q000276:immunology / Q000517:prevention & control*; D013292:Streptococcus agalactiae
D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D007074:Immunoglobulin G; D011993:Recombinant Fusion Proteins; D000469:Alkaline Phosphatase