[Effect of p14ARF gene on cell growth of human laryngeal tumor cells and expression of endogenous p53 protein].
OBJECTIVE
To explore the inhibitory effect of p14ARF on the cell growth of laryngeal carcinoma and the expression on endogenous p53.


METHODS
p14ARF cDNA was transferred to the cell line Hep-2 of squamous cell carcinoma of the larynx by gene transfer to study the cell cycles and the expression of endogenous wild type p53 using flow cytometry, polymerase chain reaction(PCR) and Western-Blotting.


RESULTS
Expression of p14ARF significantly affected the Hep-2 cell growth. The clone-forming efficiency of the Hep-2 cells transferred with p14ARF was 57%, compared with empty vector pcDNA3. The number of 48 hours after transfer with p14ARF cDNA at both G0/G1 and G2/M was two fold as the control. The expression of endogenous wild type p53 was significantly enhanced.


CONCLUSIONS
Expression of p14ARF can up-regulate the expression of endogenous wild type p53 and inhibit the Hep-2 cell growth of human laryngeal squamous cell carcinoma at both G0/G1 and G2/M.
Lei
L
L
Department of Otorhinolaryngology Head and Neck Surgery, PLA General Hospital, Beijing 100853, China. wiselei@263.net
Fan
G
G
Wang
R
R
Han
D
D
Yang
W
W
Wu
Z
Z
D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D002453:Cell Cycle; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000473:pathology; D014162:Transfection; D014407:Tumor Cells, Cultured; D025803:Tumor Suppressor Protein p14ARF / Q000235:genetics*; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis*
D025803:Tumor Suppressor Protein p14ARF; D016159:Tumor Suppressor Protein p53