title: Methodologies for the characterization of microbes in industrial environments: a review.
abstract: There is growing interest in research and development to develop novel tools to study, detect, and characterize microbes and their communities in industrial environments. However, knowledge about their validity in practical industrial use is still scarce. This review describes the advantages and limitations of traditional and molecular methods used for biofilm and/or planktonic cell studies, especially those performed with Listeria monocytogenes, Bacillus cereus, and/or Clostridium perfringens. In addition, the review addresses the importance of isolating the microorganisms from the industrial environment and the possibilities and future prospects for exploiting the described methods in the industrial environment.
authors.affiliation: VTT Biotechnology, P.O. Box 1500, 02044 VTT, Finland.
authors.forename: Johanna
authors.initials: J
authors.lastname: Maukonen
authors.forename: Jaana
authors.initials: J
authors.lastname: Mättö
authors.forename: Gun
authors.initials: G
authors.lastname: Wirtanen
authors.forename: Laura
authors.initials: L
authors.lastname: Raaska
authors.forename: Tiina
authors.initials: T
authors.lastname: Mattila-Sandholm
authors.forename: Maria
authors.initials: M
authors.lastname: Saarela
mesh_terms: D018441:Biofilms; D001709:Biotechnology / Q000379:methods* / Q000639:trends*; D004269:DNA, Bacterial / Q000032:analysis*; D007218:Industrial Microbiology / Q000379:methods* / Q000639:trends*; D008828:Microbiological Techniques; D008967:Molecular Biology / Q000379:methods*
chemical_list: D004269:DNA, Bacterial