Development and application of a pig IL-8 ELISA detection system.
Interleukin 8 (IL-8) is a chemotactic and activating chemokine, especially for neutrophils, which plays an important role in inflammatory process. A pig IL-8 specific enzyme-linked immunosorbent assay (ELISA) was developed to measure IL-8 concentrations in cell culture supernatants and biological fluids. A streptavidin-biotin amplified sandwich method uses mouse capture mAb IZ8.03 and detection biotinylated mouse mAb IZ8.04 against recombinant pig IL-8. The assay specifically and reproducibly recognizes both recombinant and natural pig IL-8. A working range of the assay is 16-1000 pg/mL and takes a mere 3.5 h of incubation time. This pig IL-8 ELISA is a suitable alternative way of measurement of IL-8 concentrations to time consuming and laborious IL-8 bioassays.
Splichal
I
I
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi, Tokyo, Japan. splichal@biomed.cas.cz
Muneta
Y
Y
Mori
Y
Y
Takahashi
E
E
D000653:Amniotic Fluid / Q000737:chemistry / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D000918:Antibody Specificity; D001710:Biotin / Q000737:chemistry / Q000276:immunology; D002478:Cells, Cultured; D016207:Cytokines / Q000032:analysis / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity; D005333:Fetus / Q000276:immunology / Q000382:microbiology; D016209:Interleukin-8 / Q000032:analysis* / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000032:analysis / Q000276:immunology; D012680:Sensitivity and Specificity; D019809:Streptavidin / Q000737:chemistry / Q000276:immunology; D013552:Swine / Q000097:blood / Q000276:immunology*
D000911:Antibodies, Monoclonal; D016207:Cytokines; D016209:Interleukin-8; D011994:Recombinant Proteins; D001710:Biotin; D019809:Streptavidin