Organization of the human FK506-binding immunophilin FKBP52 protein gene (FKBP4).
FKBP52 is a widely expressed FK506-binding immunophilin that possesses peptidylprolyl isomerase activity and a tetratricopeptide repeat involved in protein-protein interaction. FKBP52 plays an important role in steroid receptor function and is implicated in other diverse processes, including regulation of transcription, cation channel activity, and gene transfer efficiency. Reported here is the genomic organization of the human FKBP52 gene (FKBP4), which shares all but one of the same exon-intron boundaries as the structurally related immunophilin FKBP51 gene (FKBP5). Approximately 3.5 kb of 5'-flanking DNA of FKBP4 was subcloned into a luciferase reporter vector and was found to exhibit robust activity in T-47D, MCF7, and COS-7 cells. Promoter constructs with only 143 bp of upstream sequence maintained high activity. This region contains a CAAT motif sequence and consensus binding sites for Sp1, heat-shock factor, and MYC-MAX, which are conserved in the rabbit FKBP4 promoter and, when deleted, dramatically reduced promoter activity in T-47D cells.
Scammell
Jonathan G
JG
Department of Pharmacology, University of South Alabama College of Medicine, Mobile, AL 36688, USA. jscammel@jaguar1.usouthal.edu
Hubler
Tina R
TR
Denny
Wesley B
WB
Valentine
Donna L
DL
D024506:5' Flanking Region / Q000235:genetics; D001483:Base Sequence; D001665:Binding Sites; D045744:Cell Line, Tumor; D040461:Gene Components; D005822:Genetic Vectors; D016678:Genome; D006801:Humans; D008156:Luciferases / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D022021:Tacrolimus Binding Proteins / Q000235:genetics*; D014162:Transfection
D008156:Luciferases; D022021:Tacrolimus Binding Proteins; C403485:tacrolimus binding protein 4