Effect of microsomal triglyceride transfer protein gene polymorphism in the promoter region on dyslipidemia in type 2 diabetic subjects.
OBJECTIVE
To explore the relationship between microsomal triglyceride transfer protein (MTP) gene variation and diabetic dyslipidemia among Chinese.


METHODS
Using PCR restriction fragment length polymorphism (PCR-RFLP) analysis and gene sequencing, we studied the influence of a common MTP gene polymorphism in the promoter region on the apoB-containing lipoproteins in 44 Chinese type 2 diabetic subjects and 32 non-diabetic volunteers.


RESULTS
A common functional G/T polymorphism in 493 bp upstream from the transcriptional start point was detected among native Chinese. There were 41 carriers (53.9%) of the MTP-493 G/G genotype, 28 (36.8%) of the MTP-493 G/T genotype and 7 (9.3%) of the MTP-493 T/T genotype. The allele frequency of MTP-493 T in the diabetic group was 0.30. The MTP-493 T/T diabetic group had significantly higher TG (P < 0.05), VLDL-CH (P < 0.05) and smaller LDL particle size (P < 0.001) than the MTP-493 common genotype group.


CONCLUSIONS
Genetic variation in the MTP promoter is likely to be highly involved in the production of dyslipidemia in type 2 diabetic subjects.
Chen
Liming
L
Department of Diabetic Nephropathy, Metabolic Diseases Hospital, Tianjin Medical University, Tianjin 300070, China. xiefengxiang@oa.cmbchina.com
Yoshino
Gen
G
Maeda
Eiichi
E
Zeng
Shufan
S
D002352:Carrier Proteins / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005838:Genotype; D006801:Humans; D006949:Hyperlipidemias / Q000209:etiology / Q000235:genetics*; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic
D002352:Carrier Proteins; C106092:microsomal triglyceride transfer protein