The correspondence between the labeling patterns of antibody RT97, neurofilaments, microtubule associated protein 1B and tau varies with cell types and development stages of chicken retina.
The correspondence between the labeling patterns of antibody RT97, neurofilaments (NF-M and NF-H), microtubule associated protein 1B (MAP1B) and tau, were studied in the developing chicken. At embryonic day 3 (E3), intense RT97 immunoreactivity (IR) was found to be localized in cells in the region adjacent to the intraretinal space, which separates the inner and outer layers of the optic cup, and this was sustained at E8. However, this pattern changed dramatically at E12, as the intensities of RT97 IR increased in the inner retinal layer, while the outermost layers showed only weak IR. The adult stage retina showed RT97 IR within the nerve fibers of the ganglion cells, the processes of the amacrine cells and the photoreceptors. Additional immunostainings for NF-M, -H, MAP1B and tau showed that the observed changes in RT97 IRs were due to the different expressions of these proteins at different development stages.
Shin
Dong Hoon
DH
Department of Anatomy, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-Gu, Seoul 110-799, South Korea.
Lee
Kun-Sei
KS
Lee
Eunju
E
Cho
Sa Sun
SS
Kim
Jaehyup
J
Kim
Jong Wan
JW
Kwon
Bum Sun
BS
Lee
Hye Won
HW
Lee
Wang Jae
WJ
D000818:Animals; D000831:Animals, Newborn; D000906:Antibodies / Q000276:immunology; D002642:Chick Embryo; D002645:Chickens; D007150:Immunohistochemistry; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D016900:Neurofilament Proteins / Q000145:classification / Q000378:metabolism*; D010766:Phosphorylation; D012160:Retina / Q000166:cytology / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D016875:tau Proteins / Q000378:metabolism*
D000906:Antibodies; D008869:Microtubule-Associated Proteins; D016900:Neurofilament Proteins; C085284:microtubule-associated protein 1B; D016875:tau Proteins