title: Peas-Mea1-Ppp2r5d overlapping gene complex: a transposon mediated-gene formation in mammals.
abstract: Human and mouse MEA1/Mea1 is flanked by two overlapping genes, a novel PEAS/Peas in a head-to-head orientation and PPP2R5D/Ppp2r5d in a tail-to-tail orientation making a Peas-Mea1-Ppp2r5d overlapping gene complex (PMP-complex). Genomic zoo blot analyses and database searching revealed that Mea1 exists only in mammals, while Peas and Ppp2r5d are conserved in eukaryotes. Mea1 and Peas are transcribed from a testis-expressed bidirectional promoter. Mea1-Ppp2r5d overlapping segment (MPOS) contains polyadenylation signals for both genes and shows marked conservation throughout mammals. Furthermore, the MPOS occupies 3'-region of transcripts of both genes is expected to form a clover-like intramolecular structure. Mouse genomic library Screening and database searches identified two MPOS-derived sequences in Odf2 gene and RP23-86H7 cosmid clone, respectively, in which MPOS might be a core segment for the retropositions. Thus, a key role of MPOS, a short transposable element containing polyadenylation signals on both strands, in the formation of the Mea1 during mammalian evolution is suggested.
authors.affiliation: National Institute of Advanced Industrial Science and Technology, Central 6, Higashi 1-1-1, Tsukuba, Ibaraki 305-8566, Japan.
authors.forename: Yasuhide
authors.initials: Y
authors.lastname: Ohinata
authors.forename: Shizuyo
authors.initials: S
authors.lastname: Sutou
authors.forename: Youji
authors.initials: Y
authors.lastname: Mitsui
mesh_terms: D000818:Animals; D001324:Autoantigens; D001483:Base Sequence; D015139:Blotting, Southern; D018797:Cell Cycle Proteins; D017124:Conserved Sequence; D003360:Cosmids; D004251:DNA Transposable Elements; D015723:Gene Library; D006801:Humans; D008565:Membrane Proteins; D051379:Mice; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010749:Phosphoprotein Phosphatases / Q000235:genetics*; D054648:Protein Phosphatase 2; D011506:Proteins / Q000235:genetics*; D013997:Time Factors; D014018:Tissue Distribution; D014212:Tretinoin / Q000378:metabolism
chemical_list: D001324:Autoantigens; D018797:Cell Cycle Proteins; D004251:DNA Transposable Elements; C512726:Golga3 protein, mouse; C475029:Klhdc3 protein, mouse; C512727:MEA1 protein, human; C512728:Mea1 protein, mouse; D008565:Membrane Proteins; D011506:Proteins; D014212:Tretinoin; D010749:Phosphoprotein Phosphatases; C475028:Ppp2r5d protein, mouse; D054648:Protein Phosphatase 2