How much effort is required to isolate nuclear microsatellites from plants?
The attributes of codominance, reproducibility and high resolution have all contributed towards the current popularity of nuclear microsatellites as genetic markers in molecular ecological studies. One of their major drawbacks, however, is the development phase required to obtain working primers for a given study species. To facilitate project planning, we have reviewed the literature to quantify the workload involved in isolating nuclear microsatellites from plants. We highlight the attrition of loci at each stage in the process, and the average effort required to obtain 10 working microsatellite primer pairs.
Squirrell
J
J
Royal Botanic Garden Edinburgh, 20 A Inverleith Row, Edinburgh, EH3 5LR, UK. J.Squirrell@rbge.org.uk
Hollingsworth
P M
PM
Woodhead
M
M
Russell
J
J
Lowe
A J
AJ
Gibby
M
M
Powell
W
W
D017931:DNA Primers; D015723:Gene Library; D018895:Microsatellite Repeats / Q000235:genetics*; D015336:Molecular Probe Techniques; D010944:Plants / Q000235:genetics*
D017931:DNA Primers