Molecular taxonomy of dermatophytes and related fungi by chitin synthase 1 (CHS1) gene sequences.
In the present study, the nucleotide sequences of the CHS1 gene from dermatophytes and related fungi in the genera Chrysosporium, Epidermophyton, Microsporum and Trichophyton were investigated using molecular methods. About 440-bp genomic DNA fragments of the CHS1 gene from 21 species were amplified by polymerase chain reaction (PCR) and sequenced. The CHS1 nucleotide sequences of these fungi showed more than 83% similarity. The molecular taxonomy of the CHS1 gene sequences revealed that Microsporum was genetically distinct from Chrysosporium and Trichophyton, as classified by morphological characteristics.
Hirai
Asuka
A
Department of Pathobiology, Nihon University School of Veterinary Medicine, 1866, Kameino, Fujisawa, Kanagawa 252-8510, Japan.
Kano
Rui
R
Nakamura
Yuka
Y
Watanabe
Shinichi
S
Hasegawa
Atsuhiko
A
D003883:Arthrodermataceae / Q000145:classification* / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D002687:Chitin Synthase / Q000235:genetics* / Q000378:metabolism; D002912:Chrysosporium / Q000201:enzymology / Q000235:genetics; D004821:Epidermophyton / Q000201:enzymology / Q000235:genetics; D006801:Humans; D008864:Microsporum / Q000201:enzymology / Q000235:genetics; D003904:Mitosporic Fungi / Q000145:classification* / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014249:Trichophyton / Q000201:enzymology / Q000235:genetics
D002687:Chitin Synthase