Rapid separation of chlorophylls a and b and their demetallated and dephytylated derivatives using a monolithic silica C18 column and a pyridine-containing mobile phase.
A monolithic C18-bonded silica rod column (Merck Chromolith) was applied to the separation of mixtures of chlorophylls a and b and their derivatives originated by hydrolysis of the phytyl ester linkage (chlorophyllides), loss of the central Mg atom (pheophytins), or both processes (pheophorbides). Mobile phases containing two different ion-pair reagents, ammonium acetate and buffered pyridine, were tested. Both eluents achieved the resolution of the eight pigments in less than 5 min. The method based on the pyridine-containing mobile phase was applied to the separation of chlorophylls and their green coloured degradation products in senescing leaves of deciduous trees, green fruits and a marine microalga.
Garrido
José L
JL
Instituto de Investigacións Mariñas de Vigo (CSIC), Eduardo Cabello 6, E-36208 Vigo, Spain. garrido@iim.csic.es
Rodríguez
Francisco
F
Campaña
Emilia
E
Zapata
Manuel
M
D002734:Chlorophyll / Q000737:chemistry / Q000302:isolation & purification*; D000077194:Chlorophyll A; D004952:Esters; D008670:Metals / Q000737:chemistry*; D010833:Phytic Acid / Q000737:chemistry*; D011725:Pyridines / Q000737:chemistry; D012822:Silicon Dioxide
D004952:Esters; D008670:Metals; D011725:Pyridines; D002734:Chlorophyll; C037184:chlorophyll b; D012822:Silicon Dioxide; D010833:Phytic Acid; C023666:pyridine; D000077194:Chlorophyll A