title: [Relationship between p53, bax and bcl-2 expression and cell apoptosis in intrahepatic cholestasis of pregnancy].
abstract: OBJECTIVE
  To investigate the changes of gene p53, bax and bcl-2 expression and to study the role on cell apoptosis in placenta through apoptosis in placenta with intrahepatic cholestasis of pregnancy (ICP).
  
  
  METHODS
  TdT-mediated dUTP nick end labeling (TUNEL) and immuno-histochemistry methods were used for 31 placental samples with ICP and 31 normal placental samples to detect apoptosis index and the expression of p53, bax and bcl-2 in placenta tissues.
  
  
  RESULTS
  The apoptosis index in placental cytotrophoblast, syncytiotrophoblast, decidual cells and mediate cell on ICP group was significantly higher than that in control group (P < 0.01). The expression of p53 was higher than those of control. Bax expression in syncytoitrophoblast, decidual cell and mediate cell in ICP group was higher (P < 0.01), but the expression of bax in placental cytotrophoblast cell was not significantly higher (P > 0.05). The expression of bcl-2 of these positions in ICP group was lower than that in control group (P < 0.01) p53, bax and bcl-2 expressions in (chi +/- s)% in the syncytoitrophoblast cells of placenta with ICP is 75.9 +/- 8.2, 65.8 +/- 13.8, 45.3 +/- 11.3.
  
  
  CONCLUSIONS
  The placental dysfunction may closely relate to the apoptosis. Abnormal overexpression of p53, bax and the low expression of bcl-2 on placental tissue of ICP was the main reason of placental apoptosis.
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital of Xinjiang Medical University, Wulumuqi 830054, China.
authors.forename: Dong-mei
authors.initials: DM
authors.lastname: Wang
authors.forename: Qi-ying
authors.initials: QY
authors.lastname: Zhu
authors.forename: Li
authors.initials: L
authors.lastname: Ding
authors.forename: Dong
authors.initials: D
authors.lastname: Ma
mesh_terms: D000328:Adult; D017209:Apoptosis; D002780:Cholestasis, Intrahepatic / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D011247:Pregnancy; D011248:Pregnancy Complications / Q000378:metabolism / Q000473:pathology*; D011518:Proto-Oncogene Proteins / Q000032:analysis*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis*; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*; D051028:bcl-2-Associated X Protein
chemical_list: C494826:BAX protein, human; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein