Effects of estradiol, phytoestrogens, and Ginkgo biloba extracts against 1-methyl-4-phenyl-pyridine-induced oxidative stress.
Oxidative stress has been recently considered as a mediator of nerve cell death in several neurodegenerative diseases. We studied the effect of the parkinsonism-inducing toxine 1-methyl-4-phenyl-pyridine (MPP+) on several parameters of cell distress using native and neuronal PC12 cells. Then, since estrogens have been reported to prevent neuronal degeneration caused by oxidative damage, we investigated the ability of 17beta- estradiol (E2); two Ginkgo biloba extracts, EGb 761 and Cp 202; as well as two flavonoids, quercetin and kaempferol, to rescue PC12 cells submitted to MPP+- induced oxidative stress. Our results consistently show that both Ginkgo biloba extracts could prevent cell death in native and neuronal PC12 cells, while in neuronal PC12 cells also quercetin and E2 could reverse MPP+ neurotoxic effet. Western blot analysis demonstrated that MPP+ injuries might modulate dopamine transporter (DAT) protein expression but not estrogen receptor beta (ERbeta) protein expression. EGb 761 and Cp 202 also modulate DAT and ERbeta protein expression in neuronal cells. From these studies, we outline the importance of testing estrogen-like plant-derived molecules as potent antioxidants and examine their effect on protein expression.
Gagné
Benoit
B
Department of Biochemistry, Research Group in Neuroscience, Université du Québec à Trois-Rivières, Trois-Rivières, Canada.
Gélinas
Sylvie
S
Bureau
Geneviève
G
Lagacé
Bruno
B
Ramassamy
Charles
C
Chiasson
Keith
K
Valastro
Barbara
B
Martinoli
Maria-Grazia
MG
D000818:Animals; D015153:Blotting, Western; D002470:Cell Survival / Q000187:drug effects; D050483:Dopamine Plasma Membrane Transport Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D004958:Estradiol / Q000494:pharmacology*; D047629:Estrogen Receptor beta; D020441:Ginkgo biloba; D007529:Isoflavones / Q000494:pharmacology*; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D016716:PC12 Cells; D048789:Phytoestrogens; D010936:Plant Extracts / Q000494:pharmacology; D028321:Plant Preparations / Q000494:pharmacology*; D011726:Pyridinium Compounds / Q000494:pharmacology*; D011794:Quercetin / Q000494:pharmacology; D051381:Rats; D011960:Receptors, Estrogen / Q000096:biosynthesis / Q000187:drug effects / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects
D050483:Dopamine Plasma Membrane Transport Proteins; D047629:Estrogen Receptor beta; D007529:Isoflavones; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D048789:Phytoestrogens; D010936:Plant Extracts; D028321:Plant Preparations; D011726:Pyridinium Compounds; D011960:Receptors, Estrogen; C493216:Slc6a3 protein, rat; C026560:1-(4-methoxyphenyl)pyridinium; D004958:Estradiol; D011794:Quercetin