The human G protein beta4 subunit: gene structure, expression, Ggamma and effector interaction.
The aim of this study was the characterization of the human Gbeta4 subunit of heterotrimeric G proteins. Human Gbeta4 is widely expressed. Its gene is located on chromosome 3 with a genomic structure indistinguishable from that of the genes of Gbeta1 to Gbeta3, but entirely different from Gbeta5. In vitro translation co-precipitation analyses revealed that Gbeta4 can form stable dimers with Ggamma1, Ggamma2, Ggamma3, Ggamma4, Ggamma5, Ggamma7, Ggamma10, Ggamma11, Ggamma12, and Ggamma13, dimers which were also able to stimulate phospholipase beta2.
Rosskopf
Dieter
D
Institut für Pharmakologie, Universitätsklinikum Essen, Hufelandstr. 55, Germany. dieter.rosskopf@uni-essen.de
Nikula
Christiane
C
Manthey
Iris
I
Joisten
Markus
M
Frey
Ulrich
U
Kohnen
Stefanie
S
Siffert
Winfried
W
D000595:Amino Acid Sequence; D001483:Base Sequence; D002893:Chromosomes, Human, Pair 3; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D019281:Dimerization; D004789:Enzyme Activation; D005091:Exons; D020962:Heterotrimeric GTP-Binding Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D008957:Models, Genetic; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D014018:Tissue Distribution
D018076:DNA, Complementary; D012333:RNA, Messenger; D020962:Heterotrimeric GTP-Binding Proteins