[Expression of substance P receptor mRNA in nasal mucosa of rat in allergic rhinitis model].
OBJECTIVE
To study the expression of substance P receptor messenger RNA (SP-R mRNA) in nasal mucosa in allergic rhinitis (AR) rat model.


METHODS
Twenty Wistar rats free of disease were randomly divided into two groups. AR model rats were established through repeated intraperitoneal shot of ovalbumin (OV) and consequently confirmed by local challenge with OV. SP-R mRNA in nasal mucosa, obtained from two groups, were used to do reverse transcriptive polymerase chain reaction (RT-PCR) and Southern blot. beta-actin was used as a standard control through out the whole process.


RESULTS
The results showed definitely that there were positive expression of SP-R mRNA in normal nasal mucosa. This expressions increased significantly (P < 0.05) during nasal allergy.


CONCLUSIONS
Increased expression SP-R mRNA in nasal mucosa in AR model might play roles in the development of AR.
Xue
J
J
Department of Otorhinolaryngology, Second Hospital, Shanxi Medical University, Taiyuan 030001, China.
Zhao
H
H
An
Y
Y
Liang
A
A
Zhao
C
C
D000818:Animals; D005260:Female; D008297:Male; D009297:Nasal Mucosa / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D018040:Receptors, Neurokinin-1 / Q000096:biosynthesis* / Q000235:genetics; D012221:Rhinitis, Allergic, Perennial / Q000276:immunology / Q000378:metabolism*
D012333:RNA, Messenger; D018040:Receptors, Neurokinin-1