Role of mass spectrometry in the purification of peptides and proteins.
Experiments were carried out to evaluate the fractionation of proteins and peptides according to mass. Model mixtures were separated by either reversed-phase or ion-exchange chromatography with mass spectrometry-compatible mobile phase additives. Fraction collection was triggered by the mass/charge ratio of each one of the components of the mixture. Chromatography was additionally monitored with a UV-Vis detector in order to compare the new technique with generally accepted in separations. The results indicated that adequate purification is achieved by this new technique. Fraction collection triggered by changes in the mass/charge ratio reduces sample handling and analysis time. This study demonstrates the utility of mass-directed fractionation of peptides and proteins when mass spectrometry-compatible mobile phase additives are used.
Mazza
Cecilia B
CB
Waters Corporation, 34 Maple Street, Milford, MA 01757, USA.
Cavanaugh
Jie Y
JY
Neue
Uwe D
UD
Phillips
Dorothy J
DJ
D013058:Mass Spectrometry / Q000379:methods*; D010455:Peptides / Q000302:isolation & purification*; D011506:Proteins / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
D010455:Peptides; D011506:Proteins