The possibility of B-cell-dependent T-cell development.
The development of T cells is thought to be independent of B cells. However, defects in cell-mediated immunity in individuals with B-cell deficiency suggest the contrary. To test whether B cells affect T-lymphocyte development, we constructed mice with a monoclonal T-cell compartment (MT) and monoclonal B- and T-cell compartments (MBTs). In these mice, the T cells expressed a DO 11.10 transgenic (DO-T) cell receptor restricted to major histocompatibility complex (MHC) class IId. While CD4+ DO-T lymphocytes are rare in transgenic H-2b MT mice, we found that in H-2b MBT mice under the influence of B cells, DO-T lymphocytes mature into large numbers of CD4+ peripheral T cells. H-2b MBT mice have more CD4+ thymocytes than H-2b MT mice. These data are consistent with the view that B cells play some role in thymocyte development.
Keshavarzi
S
S
Department of Microbiology and Immunology, University of California, San Francisco, CA, USA.
Rietz
C
C
Simões
S
S
Shih
S
S
Platt
J L
JL
Wong
J
J
Wabl
M
M
Cascalho
M
M
D000818:Animals; D016175:B-Lymphocyte Subsets / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D018791:CD4 Lymphocyte Count; D017637:Clonal Deletion / Q000276:immunology; D006183:H-2 Antigens / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology; D007111:Immunity, Cellular; D007146:Immunoglobulin lambda-Chains / Q000235:genetics / Q000276:immunology; D007153:Immunologic Deficiency Syndromes / Q000235:genetics / Q000473:pathology; D016130:Immunophenotyping; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D018448:Models, Immunological; D010047:Ovalbumin / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
D006183:H-2 Antigens; D000949:Histocompatibility Antigens Class II; C111389:I-Ad antigen; D007146:Immunoglobulin lambda-Chains; D010047:Ovalbumin