Attenuation of axon reflexes to compound 48/80 after repeated iontophoresis of compound 48/80 in skin of the human forearm.
The aim of this study was to determine whether the iontophoretic administration of the mast cell degranulator compound 48/80 influences axon reflex vasodilatation in the skin of the human forearm. In stage 1, compound 48/80 was administered by iontophoresis to a circular site in the forearm of 9 healthy men and 8 healthy women on four occasions spread over 24 h. Two control sites were also prepared by passing the iontophoretic current through 0.9% saline. Large wheals initially developed at the compound 48/80 site in 8 of the males and in 2 of the females, but wheals were minimal in all subjects by the fourth administration. In stage 2, compound 48/80 iontophoresis provoked substantial flaring at the first control site, whereas saline iontophoresis induced only minor flaring at the second control site, indicating that compound 48/80 induced axon reflex vasodilatation. However, prior treatment with compound 48/80 inhibited flaring to compound 48/80 in subjects who initially developed wheals, consistent with mast cell degranulation. In stage 3, flaring after iontophoresis of histamine was investigated at the site of compound 48/80 pretreatment and at the second control site in 12 subjects. Flaring was impaired only slightly in 6 subjects who initially developed wheals to compound 48/80. The persistence of flaring indicates that repeated administrations of compound 48/80 did not abolish neurogenic inflammation. Transcutaneous iontophoresis of compound 48/80 may be an attractive alternative to intradermal injection in studies that aim at clarifying the function of mast cells in healthy and diseased skin.
Drummond
P D
PD
School of Psychology, Murdoch University, Perth, Australia. drummond@central.murdoch.edu.au
D000293:Adolescent; D000328:Adult; D001369:Axons / Q000187:drug effects* / Q000502:physiology; D015550:Cell Degranulation; D005260:Female; D005542:Forearm; D006632:Histamine / Q000494:pharmacology; D006636:Histamine Release; D006801:Humans; D007478:Iontophoresis; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000502:physiology; D012039:Regional Blood Flow; D012867:Skin / Q000098:blood supply* / Q000187:drug effects; D013997:Time Factors; D014664:Vasodilation / Q000187:drug effects*; D003189:p-Methoxy-N-methylphenethylamine / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*
D003189:p-Methoxy-N-methylphenethylamine; D006632:Histamine