title: Error-prone polymerase, DNA polymerase IV, is responsible for transient hypermutation during adaptive mutation in Escherichia coli.
abstract: The frequencies of nonselected mutations among adaptive Lac(+) revertants of Escherichia coli strains with and without the error-prone DNA polymerase IV (Pol IV) were compared. This frequency was more than sevenfold lower in the Pol IV-defective strain than in the wild-type strain. Thus, the mutations that occur during hypermutation are due to Pol IV.
authors.affiliation: Department of Biology, Indiana University, Bloomington, Indiana 47405, USA.
authors.forename: Joshua D
authors.initials: JD
authors.lastname: Tompkins
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Nelson
authors.forename: Jill C
authors.initials: JC
authors.lastname: Hazel
authors.forename: Stacy L
authors.initials: SL
authors.lastname: Leugers
authors.forename: Jeffrey D
authors.initials: JD
authors.lastname: Stumpf
authors.forename: Patricia L
authors.initials: PL
authors.lastname: Foster
mesh_terms: D000222:Adaptation, Physiological; D003470:Culture Media; D019951:DNA Polymerase beta / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D007785:Lactose / Q000378:metabolism; D009154:Mutation
chemical_list: D003470:Culture Media; D029968:Escherichia coli Proteins; D019951:DNA Polymerase beta; D007785:Lactose