[Studies on the chemical constituents from marine brown alga Ishige okamurai (3)].
OBJECTIVE
To study the chemical constituents of Ishige okamurai.


METHODS
Compounds were isolated by Pyricularia oryzae bioassay-guided fractionation method in combination with extraction and partitionation as well as multi-chromatography. Their structures were determined by spectral analysis and chemical evidence.


RESULTS
Seven compounds were obtained and identified as (2S)-1-O-palmitoyl-2-O-(11Z-octadecenoyl)-3-O-beta-D-galacto-pyranosyl glycerol (I), (2S)-1-O-palmitoyl-2-O-myristoyl-3-O-(6-sulfo-alpha-D- quinovopyranosyl) glycerol (II), (2S)-1-O-palmitoyl-2-O-palmitoyl-3-O-(6'-sulfo-alpha-D- quinovopyranosyl) glycerol (III) and (2S)-1-O-palmitoyl-2-O-(11Z-octadecenoyl)-3-O-(6'-sulfo-alpha-D- quinovopyranosyl) glycerol (IV), stearic acid (V), methyl myristate(VI) and palmitic acid (VII).


CONCLUSIONS
Compounds I-VI were isolated from the alga for the first time while I, II and IV are new natural products. I-IV showed activity causing morphological abnormality of P. oryzae mycelia.
Tang
Hai-feng
HF
Research Center for Marine Drugs, School of Pharmacy, Second Military Medical University, Shanghai 200433, China. haifengtang@hotmail.com
Yi
Yang-hua
YH
Yao
Xin-sheng
XS
Wu
Jiu-hong
JH
Zhang
Shu-yu
SY
Xu
Qiang-zhi
QZ
D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology; D005260:Female; D006017:Glycolipids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D018922:HL-60 Cells / Q000187:drug effects; D006801:Humans; D003904:Mitosporic Fungi / Q000187:drug effects; D015394:Molecular Structure; D000459:Phaeophyceae / Q000737:chemistry*; D013229:Stearic Acids / Q000737:chemistry / Q000302:isolation & purification; D014407:Tumor Cells, Cultured
C477175:1-O-palmitoyl-2-O-(11-octadecenoyl)-3-O-galactopyranosylglycerol; C477176:1-O-palmitoyl-2-O-myristoyl-3-O-(6-sulfoquinovopyranosyl)glycerol; D000970:Antineoplastic Agents; D006017:Glycolipids; D013229:Stearic Acids; C031183:stearic acid