Microarray gene analysis and expression profiles of Dupuytren's contracture.
Dupuytren's disease, although not altogether uncommon, has eluded scientists who have sought to explain the cause of this palmar fibroproliferative disorder. It can lead to severe limitations of hand function if left untreated. This study is the first broad genetic survey using microarray technology to find gene products that are overexpressed or underexpressed in diseased tissues. The authors found 23 genes with levels that differ consistently from control levels. Nine were selected for further verification using reverse transcription-polymerase chain reaction. These genes hold potential promise in explaining some of the demographic trends seen with disease, such as correlation with alcoholism and the striking predisposition for the male gender.
Pan
Deborah
D
Yale University School of Medicine, Division of Plastic Surgery, New Haven, CT 06520, USA.
Watson
H Kirk
HK
Swigart
Carrie
C
Thomson
J Grant
JG
Honig
Stanton C
SC
Narayan
Deepak
D
D000368:Aged; D001483:Base Sequence; D015331:Cohort Studies; D017931:DNA Primers / Q000032:analysis / Q000235:genetics; D004387:Dupuytren Contracture / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012494:Sampling Studies; D012680:Sensitivity and Specificity
D017931:DNA Primers