The CaTin1 (Capsicum annuum TMV-induced clone 1) and CaTin1-2 genes are linked head-to-head and share a bidirectional promoter.
CaTin1 was expressed relatively early in the TMV-inoculated leaves of hot pepper which is resistant to TMV-P(0) infection. Interestingly, there was another homologous gene (CaTin1-2) located in front of CaTin1 in a head-to-head fashion and they shared a single promoter. The expression profile of the CaTin1-2 was very similar to CaTin1 in all the treatments except the slower induction time compared to CaTin1 upon TMV-P(0) inoculation. The promoter analysis of CaTin1 and CaTin1-2 revealed bidirectionality both in cis-elements and activity. The CaTin1-2 promoter had two TATA-boxes, four GCC-boxes, the root responsive element, and a W1-box. The ethylene-inducible promoter activity depended on GCC-boxes and TMV-inducible activity of the CaTin1-2 promoter reached its highest activity when this promoter had a W1-box.
Shin
Ryoung
R
Graduate School of Biotechnology, Korea University, 1,5ga Anam-dong, Sungbuk-gu, Seoul 136-701, Republic of Korea.
Kim
Min Jung
MJ
Paek
Kyung-Hee
KH
D000595:Amino Acid Sequence; D002212:Capsicum / Q000235:genetics* / Q000821:virology; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D023061:Gene Order; D017343:Genes, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014027:Tobacco Mosaic Virus / Q000254:growth & development*
D010940:Plant Proteins