Purification and characterization of an endochitinase produced by Colletotrichum gloeosporioides.
The phytopathogenic fungus Colletotrichum gloeosporioides was analyzed for chitinase activity, the best production occurring at the fourth day. A 43 kDa endochitinase with specific activity of 413 U microg(-1) protein was purified corresponding to a 75% yield. The optima of temperature and pH for the enzyme were 50 degrees C and pH 7.0, respectively. The enzyme showed a high stability at 50 degrees C and pH 7.0. Values of pH from 5.0 up to 7.0 gave, at least, 50% of maximum activity, suggesting a biotechnological application. Further studies are in progress to determine the possible use of this endochitinase in biological control.
Souza
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RF
Departamento de Microbiologia Geral, Instituto de Microbiologia Prof Paulo de Góes (IMPPG), Centro de Ciências da Saúde, Universidade do Brasil, Ilha do Fundão, CEP 21941-590, Rio de Janeiro, RJ, Brazil.
Gomes
R C
RC
Coelho
R R R
RR
Alviano
C S
CS
Soares
R M A
RM
D002244:Carbon / Q000378:metabolism; D002686:Chitin / Q000378:metabolism*; D002688:Chitinases / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism*; D020231:Colletotrichum / Q000201:enzymology*; D003102:Colloids; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D007700:Kinetics
D003102:Colloids; D003470:Culture Media; D002686:Chitin; D002244:Carbon; D002688:Chitinases