[Cluster analysis of Dendrobium by RAPD and design of specific primer for Dendrobium candidum].
OBJECTIVE
To analyze the genetic relationship of Dendrobium and design a specific primer to differentiate Dendrobium candidum effectively.


METHODS
Random amplified polymorphic DNA (RAPD) technique was used to analyze the genetic relationships of 26 species of Dendrobium and one species of Ephemerantha. Dendrogram was constructed by UPGMA. According to the sequence of DNA fragment selected, Sangon 18 primer had been extended from 3' extreme to 20 bp in order to form a specific primer.


CONCLUSIONS
This primer can be used to distinguish Dendrobium candidum from other Dendrobium effectively. Such a technique provides a new way for the identification of Chinese traditional medicines.
Zhang
M
M
College of Life Sciences, Zhejiang University, Hangzhou 310012, Zhejiang, China.
Huang
H R
HR
Liao
S M
SM
Gao
J Y
JY
D016000:Cluster Analysis; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics*; D031667:Dendrobium / Q000145:classification / Q000235:genetics*; D004340:Drug Contamination; D029595:Orchidaceae / Q000235:genetics; D010946:Plants, Medicinal / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique; D013045:Species Specificity
D017931:DNA Primers; D018744:DNA, Plant