Structure determination of adeno-associated virus 2: three complete virus particles per asymmetric unit.
The atomic structure of adeno-associated virus 2 (AAV-2) has been determined to 3.0 A resolution. AAV-2 crystallized in space group P1, with unit-cell parameters a = 249.7, b = 249.7, c = 644.8 A, alpha = 90.0, beta = 101.2, gamma = 120.0 degrees. The crystals contained three full virus particles in the asymmetric unit, allowing 180-fold non-crystallographic symmetry averaging. The particle orientations were determined using the self-rotation function and found to have similar but resolvably different orientations. Approximate alignment of icosahedral and interparticle threefold screw symmetry led to a native Patterson that was interpretable in terms of approximate particle positions. Accurate positions required a Patterson correlation search that was constrained to be consistent with non-crystallographic threefold projection symmetry evident in the diffraction intensities. Initial phases to 15.0 A resolution were calculated by molecular replacement using the known structure of a distantly related homolog (23% sequence identity). Real-space averaging was performed and phases were extended from 15.0 to 3.0 A. An atomic model was fitted and refined using a simulated-annealing real-space procedure.
Xie
Qing
Q
Department of Chemistry and Biochemistry, Florida State University, Tallahassee, FL 32306-4380, USA.
Somasundaram
Thayumanasamy
T
Bhatia
Smita
S
Bu
Weishu
W
Chapman
Michael S
MS
D003460:Crystallization; D018360:Crystallography, X-Ray; D000229:Dependovirus / Q000737:chemistry*; D006860:Hydrogen Bonding; D008958:Models, Molecular; D014961:X-Ray Diffraction