[Analysis of 1H-NMR fingerprint in stem of Dendrobium loddigesii].
OBJECTIVE
To analyse the 1H-NMR finger-print of the stem of Dendrobium loddigesii.


METHODS
Silica gel column chromatography was used to separate the chemical constituents of SCE A of the stem of D. loddigesii. The characteristic signals of the H-NMR finger-print were analysed after determining the structures of the compounds isolated from SCE A.


RESULTS
1H-NMR finger-prints of the samples of D. loddigesii collected from different regions showed highly characteristic features and reproducibility. Four compounds predominant in SCE A were isolated and their structures were determined by spectral analysis as 1, 2, 3 and 4, respectively.


CONCLUSIONS
Compound 3 and 4 were isolated from D. loddigesii for the first time. The 1H-NMR finger-print of CGE A of the stem of D. loddigesii showed mainly the characteristic signals of the above four compounds and might be utilized for the original authentication of this plant.
Qin
Hai-lin
HL
Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, Guizhou, China.
Zhang
Jian-xin
JX
Wang
Zheng-tao
ZT
Yang
Xiao-sheng
XS
Xu
Luo-shan
LS
Hao
Xiao-jiang
XJ
D001593:Benzyl Compounds / Q000737:chemistry / Q000302:isolation & purification; D001632:Bibenzyls; D031667:Dendrobium / Q000737:chemistry* / Q000145:classification; D006139:Guaiacol / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D013045:Species Specificity
D001593:Benzyl Compounds; D001632:Bibenzyls; C071845:dendrophenol; C478099:gigantol; D006139:Guaiacol