Biocatalytic reduction of ketones by a semi-continuous flow process using supercritical carbon dioxide.
The immobilized resting-cell of Geotrichum candidum was used as a catalyst for the reduction of a ketone in a semi-continuous flow process using supercritical carbon dioxide for the first time; it was also applied for the asymmetric reduction of a ketone and resulted in excellent enantioselectivity (ee > 99%) and a higher space-time yield than that of the corresponding batch process.
Matsuda
Tomoko
T
Department of Materials Chemistry, Faculty of Science and Technology, Ryukoku University, Otsu, Shiga 520-2194, Japan.
Watanabe
Kazunori
K
Kamitanaka
Takashi
T
Harada
Tadao
T
Nakamura
Kaoru
K
D000098:Acetophenones / Q000737:chemistry / Q000378:metabolism; D000426:Alcohol Dehydrogenase / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism*; D002384:Catalysis; D018914:Cells, Immobilized; D003512:Cyclohexanones / Q000737:chemistry / Q000378:metabolism; D005848:Geotrichum / Q000201:enzymology / Q000378:metabolism*; D007659:Ketones / Q000737:chemistry* / Q000378:metabolism*; D010084:Oxidation-Reduction; D013237:Stereoisomerism
D000098:Acetophenones; D003512:Cyclohexanones; D007659:Ketones; C483524:o-fluoroacetophenone; D002245:Carbon Dioxide; C036468:cyclohexanone; D000426:Alcohol Dehydrogenase