Crystallization and preliminary X-ray diffraction analysis of Jun a 1, the major allergen isolated from pollen of the mountain cedar Juniperus ashei.
Jun a 1, the major allergen of pollen from the mountain cedar Juniperus ashei, has been crystallized using the hanging-drop vapor-diffusion method at 277 K. The crystals are monoclinic, space group P2(1), with unit-cell parameters a = 53.38, b = 113.48, c = 72.44 A, beta = 96.36 degrees and four molecules in the unit cell. A complete 2.5 A data set has been collected at 100 K with X-rays from a Cu Kalpha rotating-anode generator.
Liu
Dequin
D
Sealy Center for Structural Biology, Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch at Galveston, Galveston, Texas 77555-0647, USA.
Midoro-Horiuti
Terumi
T
White
Mark A
MA
Brooks
Edward G
EG
Goldblum
Randall M
RM
Czerwinski
Edmund W
EW
D000485:Allergens / Q000737:chemistry* / Q000302:isolation & purification; D052179:Antigens, Plant; D003460:Crystallization; D018653:Juniperus / Q000737:chemistry*; D008970:Molecular Weight; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification; D011133:Polysaccharide-Lyases / Q000737:chemistry; D014961:X-Ray Diffraction
D000485:Allergens; D052179:Antigens, Plant; D010940:Plant Proteins; C121951:jun a 1 protein, Juniperus ashei; D011133:Polysaccharide-Lyases; C023911:pectate lyase