Established and emerging fluorescence-based assays for G-protein function: Ras-superfamily GTPases.
Ras and Rho GTPases are signaling proteins that regulate a variety of physiological events and are intimately linked to the progression of cancer. Recently, a variety of fluorescence-based assays have been refined to monitor activation of these GTPases. This review summarizes current fluorescence-based techniques for studying Ras superfamily GTPases with an emphasis on practical examples and high-throughput applications. These techniques are not only useful for biochemical characterization of Ras superfamily members, but will also facilitate the discovery of small molecule therapeutics designed to inhibit signal transduction mediated by GTPases.
Rojas
Rafael J
RJ
Department of Pharmacology, Lineberger Comprehensive Cancer Center, Department of Biochemistry and Biophysics, The University of NC 27599-7365, USA.
Kimple
Randall J
RJ
Rossman
Kent L
KL
Siderovski
David P
DP
Sondek
John
J
D004735:Energy Transfer; D005453:Fluorescence; D020558:GTP Phosphohydrolases / Q000032:analysis* / Q000378:metabolism; D020662:Guanine Nucleotide Exchange Factors; D006801:Humans; D013050:Spectrometry, Fluorescence; D018631:ras Proteins / Q000032:analysis* / Q000378:metabolism
D020662:Guanine Nucleotide Exchange Factors; D020558:GTP Phosphohydrolases; D018631:ras Proteins