DNA ploidy in cervical lesions assessed by computed image analysis: relation to histopathology.
OBJECTIVE
To assess the different patterns of DNA nuclear content in cervical intraepithelial neoplasia.


METHODS
DNA nuclear content patterns (DNA ploidy) were assessed in 82 paraffin embedded samples diagnosed as chronic cervicitis CIN 1, CIN 2 and CIN 3 by image analysis using the CAS200 BD System. These results were compared to the consensus diagnosis of two pathologists. The chi-square test was used to evaluate the results of classificatory variables (CIN 1, CIN 2 and CIN 3).


RESULTS
Aneuploidy was found in 86% of CIN 3; 59% CIN 2; 26%% CIN 1. All samples classified as chronic cervicitis were diploid.


CONCLUSIONS
The DNA nuclear content was found to parallel the three grades of the histological WHO classification. Aneuploidy was strongly associated with the high-grade lesions; the detection of a subset aneuploidy of low grade lesions would suggest a indicate closer follow-up of these cases.
Shirata
N K
NK
Division of Pathology, Adolfo Lutz Institute, Avenida Dr. Arnaldo 355, Cerqueira Cezar 05025-902, São Paulo, SP, Brazil. shirata86@hotmail.Com
Zerbini
M C Nogueira
MC
Longatto Filho
A
A
de Mello
E Sobroza
ES
Arias
V
V
Nonogaki
S
S
Alves
V Avancini Ferreira
VA
D000782:Aneuploidy; D002908:Chronic Disease; D004247:DNA / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D016612:Paraffin Embedding; D011003:Ploidies; D002578:Uterine Cervical Dysplasia / Q000235:genetics* / Q000473:pathology; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology; D002575:Uterine Cervicitis / Q000235:genetics* / Q000473:pathology
D004273:DNA, Neoplasm; D004247:DNA