Enhancement of reactive oxygen species production from canine blood leukocytes by human recombinant interleukin-12.
A novel biological activity of human recombinant interleukin-12 (rhIL-12) on canine peripheral blood mononuclear cells (PBMC) was investigated in vitro. Canine PBMC were cultured in the presence or absence of rhIL-12 for 3 days. The reactive oxygen species (ROS) production induced by opsonized-zymosan (OZ) was then measured by a luminol-dependent chemiluminescense assay and demonstrated that the ROS production was enhanced after culture with rhIL-12. A nitro blue tetrazolium test and flowcytometry analysis revealed that canine lymphocytes, eosinophils, and monocytes were capable of ROS production, but that monocytes had the highest capacity. These results suggest that rhIL-12 enhances ROS production from canine monocytes.
Yano
Kumiko
K
Department of Veterinary Surgery, Faculty of Agriculture, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Ide
Kaori
K
Momoi
Yasuyuki
Y
Yamazoe
Kazuaki
K
Kudo
Tadaaki
T
D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D004285:Dogs; D005434:Flow Cytometry; D006801:Humans; D018664:Interleukin-12 / Q000494:pharmacology*; D007958:Leukocyte Count; D007962:Leukocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D008163:Luminescent Measurements; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015054:Zymosan / Q000494:pharmacology
D017382:Reactive Oxygen Species; D018664:Interleukin-12; D015054:Zymosan