[Preliminary study on NS5 region gene sequencing and variants of hepatitis G virus in China].
OBJECTIVE
To study the genomic specificity and variation of Hepatitis G virus(HGV) in China.


METHODS
The 141nt cDNA fragments locating at nonstructural region 5 (NS5) of HGV were obtained from the sera of 16 HGV infected patients by RT-nested-PCR using NS5 primers, then purified and directly sequenced by dideoxynucleotide chain termination method.


RESULTS
The sequence diversity near the N-terminus of the region between nt 7,820 and 7,960 was more marked than that near the C-terminus. Compared with the HGV-US isolate, the nt alterations of NS5 in Chinese isolates usually took place at nt 7,824, 7,892, 7,914, 7,917, 7,935 and in region nt 7,845-7,860, the rate of variability at one nt position was 0-89.5%. The nucleotide homology was 83.6%-95.7% (89.6% in average) and the homology of deduced amino acid sequence was 83.0-100%(91.2% in average).


CONCLUSIONS
The nucleotide and deduced amino acid sequences of NS5 of Chinese HGV are relatively conserved but are obviously different from the HGV and GBV-C reported in USA in this study.
Li
B
B
Department of Immunology, 302 Hospital of PLA, Beijing 100039.
Dong
Z
Z
Cheng
Y
Y
D000328:Adult; D005260:Female; D018178:Flaviviridae Infections / Q000821:virology*; D029407:GB virus C / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics*; D006525:Hepatitis, Viral, Human / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D017421:Sequence Analysis; D017361:Viral Nonstructural Proteins / Q000235:genetics
D017361:Viral Nonstructural Proteins