Specific identification of certain probiotic Lactobacillus rhamnosus strains with PCR primers based on phage-related sequences.
PCR primers derived from Lactobacillus rhamnosus phage Lc-Nu genome were used to screen the presence of phage-related sequences in Lb. rhamnosus strains. Several primer pairs derived from structural and replication gene regions of phage Lc-Nu amplified PCR products of expected sizes from bacterial strains revealing phage-related sequences in 10 of 11 Lb. rhamnosus strains. Strain-specific PCR primers for three probiotic Lb. rhamnosus strains were derived from these phage-related sequences for identification and detection purposes. Specificity of these primers was tested against 11 Lb. rhamnosus strains and over 40 other bacterial strains.
Brandt
Kirsi
K
Department of Biology, University of Oulu, P.O. Box 3000, Finland.
Alatossava
Tapani
T
D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D007778:Lactobacillus / Q000145:classification / Q000302:isolation & purification / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D019936:Probiotics; D012680:Sensitivity and Specificity; D013045:Species Specificity
D017931:DNA Primers; D004269:DNA, Bacterial; D004279:DNA, Viral