Evaluation of the pseudo-absorption method to quantify human stratum corneum removed by tape stripping using protein absorption.
Tape stripping is a well-known method to study the barrier function of the stratum corneum (SC) and penetration processes of topically applied substances into the horny layer. The quantification of the removed corneocytes for each tape strip is the prerequisite for these studies. The pseudo-absorption of the corneocytes was proposed as a measure for the quantification of the removed corneocyte aggregates. In this study, the pseudo-absorption of the corneocytes in the visible range (430 nm) is compared with the protein absorption in the UV range (278 nm) and an absorption at 652 nm obtained after staining of the SC proteins with Trypan blue. Both the protein absorption and the absorption measured after staining correlate well with the pseudo-absorption measured at 430 nm (R(2) = 0.92 +/- 0.04 and R(2) = 0.95 +/- 0.04, respectively).
Lindemann
U
U
Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology, Medical Faculty Charité, Humboldt University Berlin, Berlin, Germany.
Weigmann
H-J
HJ
Schaefer
H
H
Sterry
W
W
Lademann
J
J
D000328:Adult; D000078404:Epidermal Cells; D004817:Epidermis / Q000737:chemistry; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011506:Proteins / Q000737:chemistry*; D013048:Specimen Handling / Q000379:methods*; D013053:Spectrophotometry / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet; D013194:Staining and Labeling; D014466:Ultraviolet Rays
D011506:Proteins