[Detection of viral markers in liver tissue dually infected with hepatitis B and C viruses by double labelling].
OBJECTIVE
To study the distribution and mutual relation of HBV and HCV in dually infected liver tissue.


METHODS
Detected HBV and HCV nucleic acids and their antigens by immunohistochemistry and in situ hybridization, the cases with HBcAg and HCV RNA positive were tested further on single section by double labeling.


RESULTS
On the section tested by double labeling we could see that positive hepatocytes infected with HBV or HCV were mostly present in different liver lobules, even if the two viruses were seen in the same lobule, most of them were not seen within a single cell.


CONCLUSIONS
In most of hepatocytes, there was no direct interference or suppression of each other in the liver tissue dually infected with HBV and HCV. Double labeling method is useful to analyze the coinfection of two viruses in the same tissue.
Yan
H
H
Beijing Institute of Hepatitis Research, Beijing You An Hospital, Beijing 100054.
Huang
D
D
Lang
Z
Z
D004279:DNA, Viral / Q000032:analysis; D005260:Female; D016174:Hepacivirus / Q000502:physiology; D006511:Hepatitis B Antigens / Q000032:analysis*; D006515:Hepatitis B virus / Q000502:physiology; D018936:Hepatitis C Antigens / Q000032:analysis*; D006525:Hepatitis, Viral, Human / Q000821:virology*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008099:Liver / Q000821:virology*; D008297:Male; D012367:RNA, Viral / Q000032:analysis; D015163:Superinfection
D004279:DNA, Viral; D006511:Hepatitis B Antigens; D018936:Hepatitis C Antigens; D012367:RNA, Viral