An endogenous Su(Hw) insulator separates the yellow gene from the Achaete-scute gene complex in Drosophila.
The best characterized chromatin insulator in Drosophila is the Suppressor of Hairy wing binding region contained within the gypsy retrotransposon. Although cellular functions have been suggested, no role has been found yet for the multitude of endogenous Suppressor of Hairy wing binding sites. Here we show that two Suppressor of Hairy wing binding sites in the intergenic region between the yellow gene and the Achaete-scute gene complex form a functional insulator. Genetic analysis shows that at least two proteins, Suppressor of Hairy wing and Modifier of MDG4, required for the activity of this insulator, are involved in the transcriptional regulation of Achaete-scute.
Golovnin
Anton
A
Department of the Control of Genetic Processes, Institute of Gene Biology, Russian Academy of Sciences, Moscow 117334, Russia.
Biryukova
Inna
I
Birukova
Inna
I
Romanova
Olga
O
Silicheva
Margarita
M
Parshikov
Akeksander
A
Savitskaya
Ekaterina
E
Pirrotta
Vincenzo
V
Georgiev
Pavel
P
D000483:Alleles; D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000502:physiology*; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster; D004742:Enhancer Elements, Genetic; D005821:Genetic Techniques; D005838:Genotype; D008957:Models, Genetic; D016296:Mutagenesis; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics* / Q000502:physiology*; D012097:Repressor Proteins; D014157:Transcription Factors / Q000235:genetics*; D019076:Transgenes
D051792:Basic Helix-Loop-Helix Proteins; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D009687:Nuclear Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; C089199:ac protein, Drosophila; C089197:sc protein, Drosophila; C083738:su(Hw) protein, Drosophila; C050680:y protein, Drosophila