Production of landomycins in Streptomyces globisporus 1912 and S cyanogenus S136 is regulated by genes encoding putative transcriptional activators.
The regulatory genes lanI and lndI have been cloned from the landomycin A (LaA) producer Streptomyces cyanogenus S136 and from the landomycin E (LaE) producer Streptomyces globisporus 1912, respectively and both have been sequenced. A culture of S. globisporus I2-1 carrying a disrupted lndI gene did not produce LaE and other related intermediates. Complementation of S. globisporus I2-1 with either the lndI or lanI gene reconstituted LaE production indicating that LanI and LndI are involved in activation of structural genes in the respective clusters. Structural features of these regulatory genes are discussed.
Rebets
Y
Y
Department of Genetics and Biotechnology, Ivan Franko National University of L'viv, Grushevskyy st 4, L'viv 79005, Ukraine.
Ostash
B
B
Luzhetskyy
A
A
Hoffmeister
D
D
Brana
A
A
Mendez
C
C
Salas
J A
JA
Bechthold
A
A
Fedorenko
V
V
D024509:3' Flanking Region / Q000235:genetics; D024506:5' Flanking Region / Q000235:genetics; D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry; D005798:Genes, Bacterial; D010641:Phenotype; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics*; D015533:Transcriptional Activation / Q000235:genetics
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D014157:Transcription Factors; C113593:landomycin E; C064648:landomycin A