title: Immunohistochemical study of the proliferation modality of synovium in rat adjuvant arthritis.
abstract: To investigate the proliferative potencies of the two types of synovial lining cells (types A and B), we used immunohistochemical techniques under light and electron microscopy to survey the complete process of arthritis in a rat model system. Complete Freund's adjuvant (0.1 ml) containing 5 mg of heat-inactivated Mycobacterium butyricum was administered intradermally into the right hind paws of 25 male Sprague-Dawley rats. Five animals were killed at weeks 1, 2, 3, and 4 after the immunological challenge, respectively, and the infrapatellar adipose synovium was removed from each animal. Using proliferative cell nuclear antigen (PCNA) as a cell proliferation marker, we quantified the locations of proliferative cells under light microscopy and then identified the cell type with immunoelectron microscopy. The number of PCNA-positive cells was high in the deep layer at week 2, and most of them were determined to be B cells. At week 3, the number of PCNA-positive cells increased in the superficial layer, and most were identified as A cells. Our results support the idea that cells of the synovial lining proliferate in situ during the course of arthritis.
authors.affiliation: Department of Orthopaedic Surgery, Jichi Medical School, 3311-1 Yakushiji, Minamikawachi-machi, Kawachi-gun, Tochigi 329-0498, Japan.
authors.forename: Munetada
authors.initials: M
authors.lastname: Kamimoto
authors.forename: Motoshi
authors.initials: M
authors.lastname: Kikuchi
authors.forename: Takashi
authors.initials: T
authors.lastname: Yashiro
authors.forename: Aki
authors.initials: A
authors.lastname: Nihe
authors.forename: Yusei
authors.initials: Y
authors.lastname: Kariya
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Hoshino
mesh_terms: D000818:Animals; D001168:Arthritis / Q000378:metabolism* / Q000473:pathology; D006965:Hyperplasia; D007150:Immunohistochemistry; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013583:Synovial Membrane / Q000166:cytology / Q000378:metabolism* / Q000473:pathology
chemical_list: D018809:Proliferating Cell Nuclear Antigen