Lactic dehydrogenase virus infection reduces the expulsion of the nematode Nippostrongylus brasiliensis.
The interleukin (IL)-13-mediated goblet cell response is the major host effector system involved in the expulsion of Nippostrongylus brasiliensis. Lactic dehydrogenase virus (LDV) induced higher levels of N. brasiliensis egg production compared with controls, but the effect of LDV infection on worm expulsion of, and goblet cell and IL-13 responses to, N. brasiliensis have not been studied. In this study, the effects of LDV infection on these host responses against N. brasiliensis were examined. Mice with chronic LDV infection showed significantly lower worm expulsion rates than non-LDV-infected mice after N. brasiliensis infection, and there were no significant differences in the ratio of female versus male adult worms between control and LDV-infected mice. The number of goblet cells in LDV-infected mice was significantly lower than that in controls. In addition, the levels of IL-13 gene expression in lymph nodes were significantly lower in LDV-infected mice compared with controls. These results suggest that LDV infection reduces the protective immune responses against N. brasiliensis infection by the suppression of IL-13 production.
Morimoto
M
M
Laboratory of Veterinary Pathology, Faculty of Agriculture, Yamaguchi University, 1-1677 Yoshida, Yamaguchi 753-8515, Japan.
Yamamoto
N
N
Hayashi
T
T
D000818:Animals; D018174:Arterivirus Infections / Q000150:complications*; D005260:Female; D020397:Goblet Cells / Q000166:cytology; D018793:Interleukin-13 / Q000032:analysis; D007421:Intestine, Small / Q000469:parasitology; D007772:Lactate dehydrogenase-elevating virus; D008168:Lung / Q000469:parasitology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009559:Nippostrongylus / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D051381:Rats; D017207:Rats, Sprague-Dawley; D017206:Strongylida Infections / Q000150:complications / Q000276:immunology* / Q000469:parasitology
D018793:Interleukin-13