A unique mechanism for protein processing and degradation in Arabidopsis thaliana.
Precursor protease vesicles are plant-specific compartments containing precursors of enzymes that are thought to participate in the degradation of cellular components in organs undergoing senescence. We report in vivo evidence that the precursor protease vesicle-localized vacuolar processing enzyme-gamma (VPEgamma) is critical for maturation of the plant vacuolar protease AtCPY. We also provide biochemical and functional evidence that VPEgamma is involved in degradation of the vacuolar invertase AtFruct4 in aging tissues. Moreover, a proteomics-based approach identified various proteins found in the vacuoles of aging vpegamma mutants but not in WT plants, suggesting a unique role of VPEgamma in protein processing and degradation in Arabidopsis.
Rojo
Enrique
E
Department of Botany and Plant Sciences and Center for Plant Cell Biology, University of California, Riverside, CA 92521, USA.
Zouhar
Jan
J
Carter
Clay
C
Kovaleva
Valentina
V
Raikhel
Natasha V
NV
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002268:Carboxypeptidases / Q000378:metabolism; D043402:Cathepsin A; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D017343:Genes, Plant; D006026:Glycoside Hydrolases / Q000378:metabolism; D016253:Microscopy, Immunoelectron; D016254:Mutagenesis, Insertional; D009154:Mutation; D011499:Protein Processing, Post-Translational; D014617:Vacuoles / Q000378:metabolism; D043324:beta-Fructofuranosidase
D029681:Arabidopsis Proteins; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase; D002268:Carboxypeptidases; D043402:Cathepsin A; D003546:Cysteine Endopeptidases; C098280:vacuolar processing enzyme gamma, Arabidopsis