Immunochemical detection of Salmonella group B, D and E using an optical surface plasmon resonance biosensor.
A surface plasmon resonance biosensor (Biacore) was used to detect Salmonella through antibodies reacting with Salmonella group A, B, D and E (Kauffmann-White typing). In the assay designed, anti-Salmonella antibodies immobilized to the biosensor surface were allowed to bind injected bacteria followed by a pulse with soluble anti-Salmonella immunoglobulins to intensify the signal. No significant interference was found for (mixtures of) 30 non-Salmonella serovars at 10(9) CFU ml(-1). A total of 53 Salmonella serovars were successfully detected at 1 x 10(7) CFU ml(-1), except those of groups C, G, L and P, as expected. The cut-off point was determined with an equicellular mixture of Salmonella enteritidis and Salmonella typhimurium at a final amount of 1.7 x 10(3) CFU per test portion. Although further work is needed to cover the detection of all relevant Salmonella serovars in food-producing animals and food products, this work demonstrates the merits of this alternative biosensor approach in terms of automation, sensitivity, specificity, simple handling and limited hands-on time.
Bokken
Gertie C A M
GC
Department of Public Health and Food Safety, Faculty of Veterinary Medicine, Utrecht University, Utrecht, PO Box 80175, 3508 TD Utrecht, The Netherlands.
Corbee
Ronald J
RJ
van Knapen
Frans
F
Bergwerff
Aldert A
AA
D000818:Animals; D000907:Antibodies, Bacterial; D000918:Antibody Specificity; D005506:Food Contamination; D005516:Food Microbiology; D007150:Immunohistochemistry; D012475:Salmonella / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000379:methods*
D000907:Antibodies, Bacterial