Killing activity of micafungin against Aspergillus fumigatus hyphae assessed by specific fluorescent staining for cell viability.
We studied the anti-Aspergillus activity of micafungin by using two fluorescent dyes to detect cell viability. Micafungin induced flattened hyphae, caused by the bursting of cells, which had lost their viability. Micafungin has killing activity against actively growing hyphae, even though it is not fungicidal against the whole burden of Aspergillus fumigatus.
Watabe
Etsuko
E
Medicinal Biology Research Laboratories, Fujisawa Pharmaceutical Co., Ltd., Yodogawa-ku, Osaka 532-8514, Japan. etsuko_watabe@po.fujisawa.co.jp
Nakai
Toru
T
Matsumoto
Satoru
S
Ikeda
Fumiaki
F
Hatano
Kazuo
K
D000935:Antifungal Agents / Q000494:pharmacology*; D001232:Aspergillus fumigatus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D001463:Barbiturates / Q000378:metabolism; D054714:Echinocandins; D005452:Fluoresceins / Q000378:metabolism; D005456:Fluorescent Dyes; D025301:Hyphae / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D007555:Isoxazoles / Q000378:metabolism; D055666:Lipopeptides; D008074:Lipoproteins / Q000494:pharmacology*; D000077551:Micafungin; D008856:Microscopy, Fluorescence; D008857:Microscopy, Interference; D010456:Peptides, Cyclic / Q000494:pharmacology*
D000935:Antifungal Agents; D001463:Barbiturates; D054714:Echinocandins; D005452:Fluoresceins; D005456:Fluorescent Dyes; D007555:Isoxazoles; D055666:Lipopeptides; D008074:Lipoproteins; D010456:Peptides, Cyclic; C052795:bis(1,3-dibutylbarbiturate)trimethine oxonol; C084577:5-carboxyfluorescein diacetate; D000077551:Micafungin