[Research on the identification of penis et testis cervi with molecular taxonomy].
OBJECTIVE
To make the kit with witch to identify Penis et Testis Cervi with molecular taxonomy.


METHODS
The mtDNA of sika and red deer from different areas was amplified by PCR and sequenced. Compared with the mtDNA of bovine and horse from witch the false medicines were made, characteristic segments of deer were found. We selected one as the species distinctive PCR primer of deer.


RESULTS
The kit made up with this primer and related reagents could be used to discern Penis et Testis Cervi from the false medicine.


CONCLUSIONS
It is a scientific, steady, accurate and convenient way to identify Penis et Testis Cervi with molecular taxonomy.
Tang
Shuang-yan
SY
Yunnan Provincial Institute for Drug Control, Kunming 650011, Yunnan, China.
Fu
Wen
W
Chen
Yong-jiu
YJ
Wang
Jian-yun
JY
Jiang
Xu
X
Zhang
Ya-ping
YP
D000818:Animals; D002417:Cattle / Q000235:genetics; D004247:DNA / Q000235:genetics*; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics; D003670:Deer / Q000145:classification / Q000235:genetics*; D004340:Drug Contamination; D006736:Horses / Q000235:genetics; D008297:Male; D008419:Materia Medica / Q000737:chemistry*; D010413:Penis / Q000737:chemistry*; D013737:Testis / Q000737:chemistry
D017931:DNA Primers; D004272:DNA, Mitochondrial; D008419:Materia Medica; D004247:DNA