Cloning genomic INGAP: a Reg-related family member with distinct transcriptional regulation sites.
The protein product of hamster islet neogenesis-associated protein (INGAP) cDNA induces new pancreatic islet development. Manipulation of this process provides a new therapeutic strategy for the treatment of diabetes. As regulators of INGAP gene expression are unknown over 6 kb of hamster genomic INGAP has been cloned. Sequence analysis has identified a 3 kb 5-prime region with core promoter elements that is rich in transcription factor binding sites and six exons for the coding region. Analysis of promoter activity reveals stimulus-responsive DNA elements which have been identified though deletion analysis. Comparison of transcription factor binding sites in INGAP to the related gene RegIIIdelta exposes potential sites for differential gene regulation.
Taylor-Fishwick
David A
DA
Department of Medicine, The Leonard Strelitz Diabetes Institutes, Eastern Virginia Medical School, 855 W. Brambleton Avenue, Norfolk, VA 23510, USA. Taylord@evms.edu
Rittman
Sharon
S
Kendall
Hidayah
H
Roy
Lipika
L
Shi
Wenjing
W
Cao
Yong
Y
Pittenger
Gary L
GL
Vinik
Aaron I
AI
D000818:Animals; D000951:Antigens, Neoplasm; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D014408:Biomarkers, Tumor; D002460:Cell Line; D003001:Cloning, Molecular; D006224:Cricetinae; D004247:DNA / Q000235:genetics; D003920:Diabetes Mellitus / Q000628:therapy; D005786:Gene Expression Regulation; D005809:Genes, Regulator; D017930:Genes, Reporter; D006801:Humans; D007438:Introns; D007515:Islets of Langerhans / Q000254:growth & development / Q000378:metabolism; D037181:Lectins, C-Type; D008969:Molecular Sequence Data; D000073718:Pancreatitis-Associated Proteins; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics*; D017384:Sequence Deletion; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D037181:Lectins, C-Type; D000073718:Pancreatitis-Associated Proteins; D011506:Proteins; C000612141:REG3A protein, human; C490253:Reg3b protein, mouse; C088549:RegIII protein, mouse; D004247:DNA