Fluorescence assays for high-throughput screening of protein kinases.
Protein kinases comprise one of the most important group of targets for drug discovery research today. Methods to identify novel kinase inhibitors by high-throughput screening have evolved rapidly in recent years. An important aspect is the availability of fluorescent probes that can be applied in a homogeneous, or mix-and-measure, assay format. Here, we illustrate the application of fluorescence read-out technologies for kinase targets in light of our own experiences in assay development and high-throughput screening.
Zaman
G J R
GJ
Lead Discovery Unit, N V Organon, The Netherlands. guido.zaman@organon.com
Garritsen
A
A
de Boer
Th
T
van Boeckel
C A A
CA
D000818:Animals; D020650:Combinatorial Chemistry Techniques / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D005470:Fluorometry / Q000379:methods*; D006801:Humans; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000032:analysis* / Q000378:metabolism
D005456:Fluorescent Dyes; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases