title: [Optimization of conditions for storage and cultivation of the fungus Claviceps sp.--a producer of the ergot alkaloid agroclavine].
abstract: Conditions of agroclavine biosynthesis by the mutant Claviceps sp. strain s 106 were studied. The content of agroclavine was maximum (1.5-2 g/l) on days 15-16 of cultivation in the complex medium T25, containing sucrose, citric acid, and yeast extract. Agroclavine was the major component of the alkaloid fraction (90-95%). Storage of the culture at -70 degrees C in T25 supplemented by 7% glycerol provided a stable level of alkaloid formation.
authors.affiliation: Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Oblast, 142290 Russia.
authors.forename: L V
authors.initials: LV
authors.lastname: Boĭchenko
authors.forename: N F
authors.initials: NF
authors.lastname: Zelenkova
authors.forename: M U
authors.initials: MU
authors.lastname: Arinbasarov
authors.forename: T A
authors.initials: TA
authors.lastname: Reshetilova
mesh_terms: D000388:Air; D019343:Citric Acid; D002967:Claviceps / Q000254:growth & development / Q000378:metabolism*; D015925:Cryopreservation; D003470:Culture Media; D004873:Ergolines / Q000378:metabolism*; D004876:Ergot Alkaloids / Q000096:biosynthesis*; D013395:Sucrose; D013997:Time Factors; D015003:Yeasts
chemical_list: D003470:Culture Media; D004873:Ergolines; D004876:Ergot Alkaloids; D019343:Citric Acid; D013395:Sucrose; C004465:agroclavine