title: [The experimental study of intratemporal facial nerve regeneration in chitin chamber].
abstract: OBJECTIVE
  To investigate the feasibility of intratemporal facial nerve regeneration in a chitin chamber.
  
  
  METHODS
  A left 6 mm intratemporal facial nerve gap was created in adult white rabbit, and a total of 48 animals were used. The proximal and distal stumps were bridged with chitin chamber (experimental group) and silicone chamber (control group). Regeneration of the nerve was assessed by gross observation, nerve electrophysiological test, histological examination and digital morphological analysis in both groups.
  
  
  RESULTS
  1. Three months after implantation, the proximal transected neural stump was connected with the distal stump in the chitin chamber. Five months after operation, the regenerated nerve exhibited a more nature fascicular organization and the chitin tubes were completely absorbed. 2. Nerve evoked potentials showed better conduction ability in the chitin chamber. There were no significant difference in electrophysiological indices between the two chamber groups. 3. Most of the regenerated nerve fibers were myelinated nerve fibers, and axons and myelins had similar histograms between the experimental and control groups. 4. Five months after operation, the mean percentage of recovery of the regenerated nerve fiber area, axonal area and axonal number were more than 71% in the experimental group. There was no significant difference between the control and experimental groups.
  
  
  CONCLUSIONS
  These results suggested that intratemporal facial nerve could regenerate effectively within the chitin chamber in rabbits and such kind of chamber may be clinically useful.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, People's Hospital of Wuhan University, Wuhan 430060, China. caip@eyou.com
authors.forename: P
authors.initials: P
authors.lastname: Cai
authors.forename: G
authors.initials: G
authors.lastname: Liu
authors.forename: Q
authors.initials: Q
authors.lastname: Hua
authors.forename: Z
authors.initials: Z
authors.lastname: Wu
authors.forename: B
authors.initials: B
authors.lastname: Xiao
mesh_terms: D000818:Animals; D002686:Chitin / Q000494:pharmacology*; D004594:Electrophysiology; D005154:Facial Nerve / Q000473:pathology / Q000502:physiology*; D020220:Facial Nerve Injuries / Q000503:physiopathology; D005260:Female; D008297:Male; D009416:Nerve Regeneration / Q000187:drug effects*; D011817:Rabbits; D013701:Temporal Bone / Q000294:innervation*
chemical_list: D002686:Chitin