Improving the quality of BIACORE-based affinity measurements.
Although the BIACORE technology has become the standard method for measuring the affinity of antigen-antibody interactions, many users of the technology do not apply the most advanced experimental design and data processing methods that are now available. In addition, many published reports fail to provide the experimental details that are necessary to assess the reliability of the affinity data that are presented. This review describes the experimental conditions that should be used to ensure that reliable biosensor data are collected. There is little justification for the belief that ELISA methods provide in principle more reliable affinity parameters than biosensor technology.
Van Regenmortel
M H V
MH
Ecole Supérieure de Biotechnologie de Strasbourg, CNRS, Illkirch, France. vanregen@esbs.u-strasbg.fr
D000915:Antibody Affinity; D000937:Antigen-Antibody Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D006801:Humans; D011786:Quality Control; D020349:Surface Plasmon Resonance