[Study on supercritical CO2 fluid extraction and separation of components from fruits of Schisandra sphenanthera].
OBJECTIVE
To extract and separate deoxyschisandrin, gamma-schisandrin, etc in fruits of Schisandra sphenanthera by supercritical carbon dioxide fluid extraction technology.


METHODS
Optimum extraction conditions were studied by orthogonal tests and deoxyschisandrin, gamma-schisandrin, wuweizi chun A, wuweizi ester A were analyzed by HPLC.


CONCLUSIONS
The optimum extraction conditions are pressure 21 MPa, temperature 37 degrees C and flow rate of CO2 5 L.min-1, according to the rate of extraction.
Yang
S B
SB
Research Institute of Chinese Materia Medica of Zhejiang, Hangzhou 310023, Jiangsu, China.
D002245:Carbon Dioxide; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D025924:Chromatography, Supercritical Fluid / Q000379:methods; D034242:Cyclooctanes / Q000302:isolation & purification*; D005638:Fruit / Q000737:chemistry; D017705:Lignans / Q000302:isolation & purification*; D010946:Plants, Medicinal / Q000737:chemistry*; D011083:Polycyclic Compounds / Q000302:isolation & purification*; D032141:Schisandra / Q000737:chemistry*
D034242:Cyclooctanes; D017705:Lignans; D011083:Polycyclic Compounds; C015499:schizandrin B; D002245:Carbon Dioxide; C034734:schizandrin A