title: Mutans streptococci strains prevalence before and after cavity preparation during Atraumatic Restorative Treatment.
abstract: Critics argue that all carious dentine is not removed from the hand-prepared cavity during the Atraumatic Restorative Treatment (ART) procedure, and that the caries process is soon resumed. The aim of this study was to determine the effectiveness of ART in removing carious tissue, by investigating the numbers of mutans streptococci and lactobacilli, with emphasis on the prevalence of Streptococcus mutans and Streptococcus sobrinus strains before, and after ART treatment of dental caries. Two microbiology samples were collected. The first sample was removed from the centre of the carious lesion at the enamel-dentine junction, and the second was collected from the centre of the hard cavity wall above the pulp, after the soft infected dentine had been manually removed. A total of 71 mutans streptococci isolates from 31 children and 40 carious teeth were subcultured, biochemically characterised and genotyped by the arbitrarily primed polymerase chain reaction (AP-PCR). Results showed a significant decrease in TVC (P<0.0001), mutans streptococci (P < 0.0001) and lactobacilli (P = 0.0002) after cavity preparation. AP-PCR identified S. mutans strains that were undetectable during biotyping, and divided clinical isolates into two main clusters. In all, 63% (45/71) of isolates from the carious lesions comprised S. mutans strains. After cavity preparation, this was reduced to 35% (25/71), of which 30% (21/71) were S. mutans and the remaining 6% (4/71) S. sobrinus strains. The number of mutans streptococci strains was below detectable levels in 19 of the prepared cavities. The significant decrease in bacteria after manual cavity preparation demonstrates the reliability of a standardized ART technique, yet the presence of S. mutans strains shows that the effectiveness of the ART procedure can vary during treatment and between dental practitioners.
authors.affiliation: Dental Research Institute of the Medical Research Council and University of the Witwatersrand, Johannesburg, South Africa. toics@dentistry.wits.ac.za
authors.forename: C S
authors.initials: CS
authors.lastname: Toi
authors.forename: M
authors.initials: M
authors.lastname: Bönecker
authors.forename: P E
authors.initials: PE
authors.lastname: Cleaton-Jones
mesh_terms: D002648:Child; D004269:DNA, Bacterial / Q000032:analysis; D003731:Dental Caries / Q000382:microbiology; D003737:Dental Cavity Preparation / Q000379:methods*; D003793:Dental Restoration, Permanent / Q000379:methods*; D003804:Dentin / Q000382:microbiology*; D006801:Humans; D007778:Lactobacillus / Q000302:isolation & purification; D019060:Minimally Invasive Surgical Procedures; D019105:Random Amplified Polymorphic DNA Technique; D013295:Streptococcus mutans / Q000302:isolation & purification*; D016986:Streptococcus sobrinus / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial