title: The rat STSL locus: characterization, chromosomal assignment, and genetic variations in sitosterolemic hypertensive rats.
abstract: BACKGROUND
  Elevated plant sterol accumulation has been reported in the spontaneously hypertensive rat (SHR), the stroke-prone spontaneously hypertensive rat (SHRSP) and the Wistar-Kyoto (WKY) rat. Additionally, a blood pressure quantitative trait locus (QTL) has been mapped to rat chromosome 6 in a New Zealand genetically hypertensive rat strain (GH rat). ABCG5 and ABCG8 (encoding sterolin-1 and sterolin-2 respectively) have been shown to be responsible for causing sitosterolemia in humans. These genes are organized in a head-to-head configuration at the STSL locus on human chromosome 2p21.
  
  
  METHODS
  To investigate whether mutations in Abcg5 or Abcg8 exist in SHR, SHRSP, WKY and GH rats, we initiated a systematic search for the genetic variation in coding and non-coding region of Abcg5 and Abcg8 genes in these strains. We isolated the rat cDNAs for these genes and characterized the genomic structure and tissue expression patterns, using standard molecular biology techniques and FISH for chromosomal assignments.
  
  
  RESULTS
  Both rat Abcg5 and Abcg8 genes map to chromosome band 6q12. These genes span ~40 kb and contain 13 exons and 12 introns each, in a pattern identical to that of the STSL loci in mouse and man. Both Abcg5 and Abcg8 were expressed only in liver and intestine. Analyses of DNA from SHR, SHRSP, GH, WKY, Wistar, Wistar King A (WKA) and Brown Norway (BN) rat strains revealed a homozygous G to T substitution at nucleotide 1754, resulting in the coding change Gly583Cys in sterolin-1 only in rats that are both sitosterolemic and hypertensive (SHR, SHRSP and WKY).
  
  
  CONCLUSIONS
  The rat STSL locus maps to chromosome 6q12. A non-synonymous mutation in Abcg5, Gly583Cys, results in sitosterolemia in rat strains that are also hypertensive (WKY, SHR and SHRSP). Those rat strains that are hypertensive, but not sitosterolemic (e.g. GH rat) do not have mutations in Abcg5 or Abcg8. This mutation allows for expression and apparent apical targeting of Abcg5 protein in the intestine. These rat strains may therefore allow us to study the pathophysiological mechanisms involved in the human disease of sitosterolemia.
authors.affiliation: Division of Endocrinology, Diabetes and Medical Genetics, Medical University of South Carolina, STR 541, 114 Doughty Street, Charleston, SC 29403, USA. yuh@musc.edu
authors.forename: Hongwei
authors.initials: H
authors.lastname: Yu
authors.forename: Bhaswati
authors.initials: B
authors.lastname: Pandit
authors.forename: Eric
authors.initials: E
authors.lastname: Klett
authors.forename: Mi-Hye
authors.initials: MH
authors.lastname: Lee
authors.forename: Kangmo
authors.initials: K
authors.lastname: Lu
authors.forename: Khalil
authors.initials: K
authors.lastname: Helou
authors.forename: Ikuo
authors.initials: I
authors.lastname: Ikeda
authors.forename: Nami
authors.initials: N
authors.lastname: Egashira
authors.forename: Masao
authors.initials: M
authors.lastname: Sato
authors.forename: Richard
authors.initials: R
authors.lastname: Klein
authors.forename: Ashok
authors.initials: A
authors.lastname: Batta
authors.forename: Gerald
authors.initials: G
authors.lastname: Salen
authors.forename: Shailendra B
authors.initials: SB
authors.lastname: Patel
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D014644:Genetic Variation; D017404:In Situ Hybridization, Fluorescence; D008074:Lipoproteins / Q000235:genetics*; D020125:Mutation, Missense; D010840:Phytosterols / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011918:Rats, Inbred SHR / Q000235:genetics*; D011921:Rats, Inbred WKY / Q000235:genetics*; D017207:Rats, Sprague-Dawley / Q000235:genetics; D017208:Rats, Wistar / Q000235:genetics; D012855:Sitosterols / Q000097:blood*; D013045:Species Specificity
chemical_list: D008074:Lipoproteins; D010840:Phytosterols; D012333:RNA, Messenger; D012855:Sitosterols