[Determination of gossypol in cotton root bark by HPLC].
OBJECTIVE
To establish a method for the determination of gossypol in cotton root bark.


METHODS
Samples were extracted with acetone, and determined on a C18 column with the mobile phase (Acetonitrile-0.2% phosphoric acid, 85:15) and UV-235 nm detector.


RESULTS
The recovery rate was 97.6%, RSD 1.59% (n = 5).


CONCLUSIONS
This method can be used for the determination of gossypol in cotton root bark and the content of gossypol in three different species has been determined.
Cui
Guang-hong
GH
Hubei College of Traditional Chinese Medicine, Hubei, Wuhan 430061. guanghongcui@263.net
Chun
Jia-chun
JC
Cai
Da-yong
DY
D002851:Chromatography, High Pressure Liquid; D003368:Gossypium / Q000737:chemistry*; D006072:Gossypol / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*
D006072:Gossypol