title: Automated pneumococcal MLST using liquid-handling robotics and a capillary DNA sequencer.
abstract: Multilocus sequence typing (MLST) is used by the Scottish Meningococcus and Pneumococcus Reference Laboratory (SMPRL) as a routine method for the characterization of certain bacterial pathogens. The SMPRL recently started performing MLST on strains of Streptococcus pneumoniae, and here we describe a fully automated method for MLST using a 96-well-format liquid-handling robot and a 96-capillary automated DNA sequencer.
authors.affiliation: Scottish Meningococcus and Pneumococcus Reference Laboratory, North Glasgow University Hospital NHS Trust, Department of Microbiology, Stobhill Hospital, Balornock Rd., Glasgow, G21 3UW, UK.
authors.forename: Johanna
authors.initials: J
authors.lastname: Jefferies
authors.forename: Stuart C
authors.initials: SC
authors.lastname: Clarke
authors.forename: Mathew A
authors.initials: MA
authors.lastname: Diggle
authors.forename: Andrew
authors.initials: A
authors.lastname: Smith
authors.forename: Chris
authors.initials: C
authors.lastname: Dowson
authors.forename: Tim
authors.initials: T
authors.lastname: Mitchell
mesh_terms: D001331:Automation; D015373:Bacterial Typing Techniques / Q000379:methods*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods; D013296:Streptococcus pneumoniae / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial