title: Assessment of progesterone concentration using enzymeimmunoassay, for early pregnancy diagnosis in sheep and goats.
abstract: The objective of this study was to determine a value of serum progesterone (P4) concentration, assessed using an enzymeimmunoassay (EIA), for the early distinction between pregnant and non-pregnant ewes and goats. Adult, non-lactating ewes of Chios (n=53), Berrichon (n=30) and Sfakia (n=45) breeds were synchronized during the breeding season with progestagens and gonadotrophins and mated to fertile rams (Experiment I). Adult, lactating goats of Swiss breeds (Alpine and Saanen, n=104) and indigenous Greek breed (n=45) were synchronized during the transitional season with progestagens, PGF2alpha and gonadotrophins. Cervical artificial insemination (AI) with fresh semen was applied once, 42-44 h after sponge removal (Experiment II). Jugular blood samples were collected on day 19 after sponge removal (ewes) or on day 21 after AI (goats) and serum P4 concentration was determined by EIA. Progesterone concentrations >/=1.0, >/=1.5, >/=2.5 and >/=4.0 ng/ml were tested as indicative of pregnancy. Pregnancy diagnosis was verified on birth. In the case of sheep, using a discriminatory level of 2.5 ng/ml, overall accuracy of pregnancy diagnosis was 91.4% and predictive value of negative and positive diagnoses were 98.3 and 85.3%, respectively. In the case of goats, predictive value of negative diagnosis was 95.8 and 94.0% and predictive value of positive diagnosis 71.3 and 71.7%, for 1.5 and 2.5 ng/ml, respectively; overall accuracy was 79.2% using either level. The other discriminatory levels tested did not improve these results. A significant positive correlation was observed between P4 concentration and the number of lambs or kids born, and further analysis indicated that this relationship is not a simple linear function. Based on the results of this study, P4 concentrations of 2.5 ng/ml in the case of ewes and 1.5-2.5 ng/ml in the case of goats, determined with EIA, are proposed as discriminatory levels between pregnant and non-pregnant animals, at an interval of one oestrous cycle after service.
authors.affiliation: Clinic of Obstetrics & AI, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece. pboscos@vet.auth.gr
authors.forename: C M
authors.initials: CM
authors.lastname: Boscos
authors.forename: F C
authors.initials: FC
authors.lastname: Samartzi
authors.forename: A G
authors.initials: AG
authors.lastname: Lymberopoulos
authors.forename: A
authors.initials: A
authors.lastname: Stefanakis
authors.forename: S
authors.initials: S
authors.lastname: Belibasaki
mesh_terms: D000818:Animals; D005260:Female; D006041:Goats; D007124:Immunoenzyme Techniques / Q000592:standards / Q000662:veterinary*; D007315:Insemination, Artificial / Q000662:veterinary; D011237:Predictive Value of Tests; D011247:Pregnancy; D011258:Pregnancy Tests / Q000592:standards / Q000662:veterinary*; D011374:Progesterone / Q000097:blood*; D012680:Sensitivity and Specificity; D012756:Sheep
chemical_list: D011374:Progesterone