In vitro hemolytic activity of Lonomia obliqua caterpillar bristle extract on human and Wistar rat erythrocytes.
Human accidental envenomation caused by skin contact with the bristles of Lonomia obliqua caterpillar causes coagulation and fibrinolysis disorders. Alterations of hematologic parameters are observed only in severe cases of envenomation, but with no clinical evidence of intravascular hemolysis. However, since we have observed intravascular hemolysis in preliminary studies using Wistar rats as an experimental model for investigating L. obliqua envenomation, the objective of the present study was to investigate the in vitro hemolytic activity of the bristle extract of L. obliqua caterpillars on human and rat erythrocytes. Our results showed that the bristle extract has indirect and direct hemolytic activity on human and rat erythrocytes, although direct hemolytic activity was only observed at higher bristle extract concentrations. We also observed that the bristle extract has a proteolytic activity on band 3 of human and rat erythrocyte membranes. Thus, crude L. obliqua bristle extract was found to contain at least two components with hemolytic activity on erythrocytes, a phospholipase enzyme and another protein with a direct activity on the erythrocyte membrane.
Seibert
Carla Simone
CS
Laboratório de Fisiopatologia, Instituto Butantan, Avenida Vital Brazil, 1500, CEP 05503-900, São Paulo SP, Brazil.
Shinohara
Elvira Maria Guerra
EM
Sano-Martins
Ida Sigueko
IS
D000818:Animals; D001180:Arthropod Venoms / Q000008:administration & dosage / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000187:drug effects*; D006461:Hemolysis / Q000187:drug effects; D006470:Hemorrhage / Q000139:chemically induced; D006801:Humans; D007915:Lepidoptera; D008297:Male; D010741:Phospholipases A / Q000008:administration & dosage / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar
D001180:Arthropod Venoms; D010741:Phospholipases A