title: [Heat shock protein 70-HBsAg complex inducing antigen-specific cytotoxic T lymphocyte immune response].
abstract: OBJECTIVE
  To explore the possibility of cell-medicated immune response induced with heat shock protein 70 (HSP70)-HBsAg protein complex in vitro.
  
  
  METHODS
  HSP70-HBsAg complex was reconstituted in vitro which was injected into mice in order to observe that whether HSP70-HBsAg would stimulate humoral and cellular immune responses. HSP70, HSP70-HBsAg complex and HBsAg were used to activate the dentritic cell (DC) individually, which would initiate homogeneic T lymphocyte to transform to cytotoxic T lymphocyte (CTL). The cytotoxicity of CTL was detected with MTT assay.
  
  
  RESULTS
  HSP70-HBsAg complex elicited both humoral and cellular immune responses against HBsAg in mice. Specific CD8+ CTL response was readily induced by HSP70-HBsAg complex and HBsAg, especially the former.
  
  
  CONCLUSIONS
  HSP70-HBsAg complex is immunogenic and HSP70 can lead to great efficient CTL response. And HSP70-HBsAg complex may be used as a protein vaccine for immunotherapy for chronic hepatitis B.
authors.affiliation: Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China.
authors.forename: Ming-li
authors.initials: ML
authors.lastname: Peng
authors.forename: Hong
authors.initials: H
authors.lastname: Ren
authors.forename: Hong-mei
authors.initials: HM
authors.lastname: Xu
authors.forename: Min
authors.initials: M
authors.lastname: Chen
authors.forename: Ning
authors.initials: N
authors.lastname: Ling
authors.forename: Yu-ling
authors.initials: YL
authors.lastname: Qin
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000276:immunology*; D006514:Hepatitis B Surface Antigens / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D009169:Mycobacterium tuberculosis / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D018840:HSP70 Heat-Shock Proteins; D006514:Hepatitis B Surface Antigens; D010455:Peptides