[Absorption and distribution of 5-aminosalicylic acid from its chitosan capsule degraded by colon bacteria-released enzymes in rats].
OBJECTIVE
To examine the absorption and distribution of 5-aminosalicylic acid (5-ASA) in rat colon after oral administration of its chitosan capsule.


METHODS
5-ASA in chitosan capsules (4.8 mg per 3 capsules) were administered orally in rats via a polyethylene cannula under light ether anesthesia. The rats in control group were given 1 ml 5-ASA carboxymethyl cellulose suspension (4.8 mg). Blood samples were obtained from the rat hearts and the colon tissues sampled at a given interval to measure the concentration of 5-ASA by HPLC.


RESULTS
The area under the curve (AUC(0-12)) in the serum samples of chitosan capsule group was 0.62 times that of the control group, while in the colon tissue, the AUC(0-12) of chitosan capsule group was as much as 3.62 times that of the control group.


CONCLUSIONS
The chitosan capsule may be a useful carrier of 5-ASA for colon-specific delivery.
Li
Guo-feng
GF
Department of Pharmaceutics, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. lgfnf@fimmu.com
Chen
Jian-hai
JH
Liu
Li-jie
LJ
Guo
Dan
D
Yan
Yuan
Y
Xu
Zhong-yuan
ZY
Chen
Zhi-liang
ZL
D000042:Absorption; D000818:Animals; D019540:Area Under Curve; D001419:Bacteria / Q000201:enzymology; D002686:Chitin / Q000008:administration & dosage* / Q000031:analogs & derivatives*; D048271:Chitosan; D002851:Chromatography, High Pressure Liquid; D003106:Colon / Q000378:metabolism* / Q000382:microbiology; D019804:Mesalamine / Q000008:administration & dosage* / Q000493:pharmacokinetics; D051381:Rats; D014018:Tissue Distribution
D002686:Chitin; D019804:Mesalamine; D048271:Chitosan