ESTs from the basidiomycete Schizophyllum commune grown on nitrogen-replete and nitrogen-limited media.
Lambda phage cDNA libraries were constructed using mRNAs from the basidiomycete Schizophyllum commune grown on media with high or low nitrogen concentrations. A total of 440 clones were sequenced, representing 373 distinct transcripts. Of these, 166 showed significant similarity to annotated genes in GenBank. Those that could be tentatively identified using BLAST searches were classified by function using the Gene Ontology (GO) database. Genes with products involved in cell-cycle processes were more frequent in the nitrogen-limited libraries, while genes with products involved in protein biosynthesis were more frequent in the nitrogen-replete library. Overall, clones showed much greater similarity to the one publicly available basidiomycete genome, Phanerochaete chrysosporium, than to any of the ascomycete genomes.
Guettler
S
S
Southeast Missouri State University Biology Department, 1 University Plaza, Cape Girardeau, MO 63701, USA.
Jackson
E N
EN
Lucchese
S A
SA
Honaas
L
L
Green
A
A
Hittinger
C T
CT
Tian
Y
Y
Lilly
W W
WW
Gathman
A C
AC
D010582:Bacteriophage lambda / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics; D003001:Cloning, Molecular; D003470:Culture Media; D020224:Expressed Sequence Tags; D005656:Fungal Proteins / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009584:Nitrogen; D012567:Schizophyllum / Q000235:genetics* / Q000254:growth & development
D003470:Culture Media; D005656:Fungal Proteins; D009584:Nitrogen