title: A rapid, automated flow cytometric method to measure activated degranulated platelets by density determination.
abstract: Platelet activation is reported to correlate with acute coronary syndromes. A platelet analysis method on the ADVIA 120 Hematology System provides rapid analysis of platelet density, reported as mean platelet component (MPC) concentration, utilizes routine hematology specimens, requires no pre-treatment, and thirty seconds to generate results. Sub-populations of platelets separated by density gradients showed excellent correlation with the ADVIA 120 MPC parameter (r = 0.997). Platelet activation induced by thrombin treatment resulted in a shift of platelets into the lowest density fraction (d</=1.068 g/mL) with a corresponding reduction in MPC from 24.7 to 20.6 g/dL, N = 4 subjects (p < 0.004). There was also excellent correlation between expression of CD62P measured by fluorescence flow cytometry and the ADVIA 120 Hematology System MPC values (r = 0.85). These results indicate that the ADVIA 120 MPC parameter may be a useful new test for assessing activated platelets and platelet density.
authors.affiliation: Bayer Diagnostics, 511 Benedict Avenue, Tarrytown, New York 10591 USA. sabrinah.chapman.b@bayer.com
authors.forename: E Sabrinah
authors.initials: ES
authors.lastname: Chapman
authors.forename: Martin
authors.initials: M
authors.lastname: Sorette
authors.forename: Edward
authors.initials: E
authors.lastname: Hetherington
authors.forename: David
authors.initials: D
authors.lastname: Zelmanovic
authors.forename: Gail
authors.initials: G
authors.lastname: Kling
authors.forename: Jacques
authors.initials: J
authors.lastname: Dugailliez
authors.forename: Nuria
authors.initials: N
authors.lastname: Pujol-Moix
authors.forename: David
authors.initials: D
authors.lastname: Okrongly
mesh_terms: D001331:Automation; D002138:Calibration; D015550:Cell Degranulation; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D019007:P-Selectin / Q000032:analysis; D015539:Platelet Activation; D010979:Platelet Function Tests / Q000295:instrumentation / Q000379:methods; D013917:Thrombin
chemical_list: D019007:P-Selectin; D013917:Thrombin