title: Localization of corticoliberin receptors in the rat brain.
abstract: In situ hybridization was used to study the distribution of corticoliberin receptors of subtypes 1 and 2 (CL-R1 and CL-R2 respectively) in different structures of the rat brain. Levels of CL-R1 mRNA in the brain were significantly greater than levels of CL-R2 mRNA, and the most intense expression of the CL-R1 gene was seen in forebrain structures, especially various neocortical, archicortical, and paleocortical regions in the cerebellar cortex. In addition, significant levels of CL-R1 mRNA expression were noted in the red nucleus and the reticular nucleus of the tegmentum. Intense expression of CL-R2 mRNA was observed in structures of the olfactory system, corticomedial parts of the amygdala, fields CA1-CA4 of the hippocampus, the ventromedial hypothalamus, and several brain stem nuclei. Moderate levels of CL-R2 mRNA were seen in the dorsolateral neostriatum. These results provide evidence that corticoliberin receptors of both subtypes are widespread in the brain. The different patterns of expression of CL-R1 and CL-R2 in the brain probably provide the basis for the functional specificity of action of corticoliberin in brain structures.
authors.affiliation: Laboratory of Neuroendocrinology, I. P. Pavlov Institute of Physiology, Russian Academy of Sciences, 6 Makarov Bank, 199034 St. Petersburg, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Rybnikova
authors.forename: M
authors.initials: M
authors.lastname: Pelto-Huikko
authors.forename: V V
authors.initials: VV
authors.lastname: Rakitskaya
authors.forename: V G
authors.initials: VG
authors.lastname: Shalyapina
mesh_terms: D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000033:anatomy & histology*; D001923:Brain Chemistry / Q000502:physiology*; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism; D017403:In Situ Hybridization; D008297:Male; D009841:Oligonucleotides; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D018019:Receptors, Corticotropin-Releasing Hormone / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D054832:Urocortins; D000099052:CRF Receptor, Type 1
chemical_list: C101531:CRF receptor type 2; D009841:Oligonucleotides; D012333:RNA, Messenger; D018019:Receptors, Corticotropin-Releasing Hormone; D054832:Urocortins; D000099052:CRF Receptor, Type 1; D003346:Corticotropin-Releasing Hormone