title: Tissue macroarray: a simple and cost-effective method for high-throughput studies.
abstract: The tissue microarray technique is a powerful technique that can examine many clinical specimens in a single slide. However, this technique requires a specialized instrument for the construction of tissue microarray block. The authors describe an alternative tissue macroarray technique for the simultaneous analysis of multiple clinical specimens on a single slide. As an example of this method, the authors assembled 12 tumor samples from 12 different paraffin blocks on a glass slide and then immunostained with hMLH1 antibody. This simple method does not need specialized equipment and should be an alternative efficient approach for large-scale clinical specimens.
authors.affiliation: Department of Pathology, The University of Texas-Houston Medical School, The University of Texas MD Anderson Cancer Center, Houston, Texas 77030, USA.
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.forename: Michael T
authors.initials: MT
authors.lastname: Deavers
authors.forename: Anais
authors.initials: A
authors.lastname: Malpica
authors.forename: Elvio G
authors.initials: EG
authors.lastname: Silva
authors.forename: Jinsong
authors.initials: J
authors.lastname: Liu
mesh_terms: D000911:Antibodies, Monoclonal; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000191:economics / Q000379:methods*; D008867:Microtomy; D010051:Ovarian Neoplasms / Q000473:pathology*; D016612:Paraffin Embedding; D013194:Staining and Labeling
chemical_list: D000911:Antibodies, Monoclonal