TAF10 (TAF(II)30) is necessary for TFIID stability and early embryogenesis in mice.
TAF10 (formerly TAF(II)30), is a component of TFIID and the TATA box-binding protein (TBP)-free TAF-containing complexes (TFTC/PCAF/STAGA). To investigate the physiological function of TAF10, we disrupted its gene in mice by using a Cre recombinase/LoxP strategy. Interestingly, no TAF10(-/-) animals were born from intercrosses of TAF10(+/-) mice, indicating that TAF10 is required for embryogenesis. TAF10(-/-) embryos developed to the blastocyst stage, implanted, but died shortly after ca. 5.5 days postcoitus. Surprisingly, trophoblast cells from TAF10(-/-) blastocysts were viable, whereas inner cell mass cells failed to survive, highlighting that TAF10 is not generally required for transcription in all cells. TAF10-deficient cells express normal levels of TBP and TAFs other than TAF10 but contain only partially formed TFIID, are endocycle arrested, and have undetectable levels of transcription. Thus, our results demonstrate that TAF10 is required for TFIID stability, cell cycle progression, and transcription in the early mouse embryo.
Mohan
William S
WS
Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, F-67404 Illkirch Cedex, CU de Strasbourg, France.
Scheer
Elisabeth
E
Wendling
Olivia
O
Metzger
Daniel
D
Tora
Làszlò
L
D000483:Alleles; D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002453:Cell Cycle; D002455:Cell Division; D002470:Cell Survival; D003433:Crosses, Genetic; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D005838:Genotype; D017403:In Situ Hybridization; D020287:In Situ Nick-End Labeling; D051379:Mice; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence; D008957:Models, Genetic; D009154:Mutation; D010641:Phenotype; D010766:Phosphorylation; D011233:Precipitin Tests; D011506:Proteins / Q000378:metabolism / Q000502:physiology; D012313:RNA / Q000378:metabolism; D045522:Recombinases; D035381:TATA-Binding Protein Associated Factors / Q000235:genetics* / Q000502:physiology*; D013997:Time Factors; D035362:Transcription Factor TFIID / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D014157:Transcription Factors; D014327:Trophoblasts / Q000378:metabolism
D011506:Proteins; D045522:Recombinases; C089768:TAF10 protein, human; D035381:TATA-Binding Protein Associated Factors; C418885:Tbn protein, mouse; D035362:Transcription Factor TFIID; D014157:Transcription Factors; D012313:RNA; D004254:DNA Nucleotidyltransferases; D001973:Bromodeoxyuridine