title: Preparative purification of soybean agglutinin by affinity chromatography and its immobilization for polysaccharide isolation.
abstract: Optimized procedures for the affinity purification of soybean agglutinin (SBA) from soybean flour, and its further immobilization, were developed. Lectin purification on galactosyl-Sepharose yielded 44.5+/-3.5 mg of pure SBA/50 g of flour. To prepare SBA adsorbents, the lectin was immobilized onto 1-cyano-4-(dimethylamino)pyridinium tetrafluoroborate (CDAP) activated Sepharose with high yields (77%). Feasibility of the use of this improved SBA adsorbent for affinity purification of Streptococcus pneumoniae capsular polysaccharides from strain 14 (CPS-14) at laboratory scale was demonstrated. Using SBA-Sepharose adsorbent (7.0 mg lectin per ml), amounts of 6.3 mg of pure CPS-14 per cycle were produced, the adsorbent being reused up to four times without loss of capacity.
authors.affiliation: Cátedra de Bioqui;mica, Facultad de Qui;mica, CC 1157, Montevideo, Uruguay. lfranco@fq.edu.uy
authors.forename: Laura
authors.initials: L
authors.lastname: Franco-Fraguas
authors.forename: Alicia
authors.initials: A
authors.lastname: Plá
authors.forename: Fernando
authors.initials: F
authors.lastname: Ferreira
authors.forename: Hugo
authors.initials: H
authors.lastname: Massaldi
authors.forename: Norma
authors.initials: N
authors.lastname: Suárez
authors.forename: Francisco
authors.initials: F
authors.lastname: Batista-Viera
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D037121:Plant Lectins / Q000302:isolation & purification*; D011134:Polysaccharides / Q000302:isolation & purification*; D030262:Soybean Proteins / Q000302:isolation & purification*; D010835:Phytohemagglutinins
chemical_list: D037121:Plant Lectins; D011134:Polysaccharides; D030262:Soybean Proteins; C028824:soybean lectin; D010835:Phytohemagglutinins