Retinal expression of clusterin in the streptozotocin-induced diabetic rat.
To assess the possible relevance of clusterin in the pathophysiology of retinopathy associated with diabetes mellitus, streptozotocin-induced diabetic rats were studied. Clusterin expression was measured in both normal and streptozotocin-induced diabetic rat retinas using Northern blotting, reverse transcription polymerase chain reaction, immunohistochemistry and Western blot analysis. The results show increased clusterin protein level and its mRNA expression 6 weeks after induction of diabetes. Clusterin was localized to the inner nuclear and ganglion cell layers of both normal and diabetic rat retinas. These data show that diabetes affects the expression of clusterin in the retina and may reflect a diabetes-induced damage and/or alterations of neural structures resulting in diabetic retinopathy.
Kim
Yoon Sook
YS
Department of Anatomy and Neurobiology, Institute of Health Science, College of Medicine, Gyeongsang National University, Chilam-dong 92, Jinju, Kyungnam 660-751, South Korea.
Kim
Young Hee
YH
Cheon
Eun Woo
EW
Park
Jong Moon
JM
Yoo
Ji Myong
JM
Kang
Sang Soo
SS
Cho
Gyeong Jae
GJ
Choi
Wan Sung
WS
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D015152:Blotting, Northern; D015153:Blotting, Western; D001835:Body Weight; D051152:Clusterin; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D003930:Diabetic Retinopathy / Q000378:metabolism* / Q000473:pathology; D006023:Glycoproteins / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D018832:Molecular Chaperones / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000378:metabolism* / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction
D001786:Blood Glucose; D051152:Clusterin; D006023:Glycoproteins; D018832:Molecular Chaperones