title: Differential effects of quercetin and resveratrol on Band 3 tyrosine phosphorylation signalling of red blood cells.
abstract: The protective effects of eating fruits and vegetables in the prevention of several degenerative pathologies have been attributed at least in part to the antioxidant and anti-inflammatory properties of polyphenols. In this study, we investigated the effects of two polyphenols, quercetin and resveratrol, on red blood cell Band 3 tyrosine phosphorylation signalling activated by peroxynitrite. Peroxynitrite is a physiological oxidant scavenged largely by the erythrocyte and formed by the reaction between nitrogen monoxide and superoxide anion. Quercetin and its structurally analogous (+)-catechin inhibited the peroxynitrite-dependent upregulation of Band 3 tyrosine phosphorylation. Quercetin was found to downregulate the activity of syk, which is upstream in the Band 3 tyrosine phosphorylation cascade, and partially prevented peroxynitrite-mediated phosphotyrosine phosphatase inhibition. Resveratrol and hydroxytyrosol, unexpectedly, amplified peroxynitrite-dependent upregulation of Band 3 tyrosine phosphorylation through the activation of lyn, a kinase of the src family. The present results clearly indicate that polyphenols may activate cell transduction pathways in different and sometimes opposite ways.
authors.affiliation: Cell Biology Department, Istituto Superiore di Sanità, Viale Regina Elena, 299-00161 Rome, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Maccaglia
authors.forename: Cinzia
authors.initials: C
authors.lastname: Mallozzi
authors.forename: Maurizio
authors.initials: M
authors.lastname: Minetti
mesh_terms: D001457:Anion Exchange Protein 1, Erythrocyte / Q000737:chemistry / Q000378:metabolism*; D000975:Antioxidants / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004792:Enzyme Precursors / Q000187:drug effects / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000201:enzymology; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D030421:Peroxynitrous Acid / Q000037:antagonists & inhibitors / Q000494:pharmacology; D010766:Phosphorylation; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000187:drug effects / Q000378:metabolism; D011794:Quercetin / Q000494:pharmacology*; D000077185:Resveratrol; D015398:Signal Transduction / Q000187:drug effects; D013267:Stilbenes / Q000494:pharmacology*; D000072377:Syk Kinase; D014443:Tyrosine / Q000378:metabolism*; D019061:src-Family Kinases / Q000187:drug effects / Q000378:metabolism
chemical_list: D001457:Anion Exchange Protein 1, Erythrocyte; D000975:Antioxidants; D004792:Enzyme Precursors; D047908:Intracellular Signaling Peptides and Proteins; D013267:Stilbenes; D030421:Peroxynitrous Acid; D014443:Tyrosine; D011794:Quercetin; D011505:Protein-Tyrosine Kinases; C000606896:SYK protein, human; D000072377:Syk Kinase; C060902:lyn protein-tyrosine kinase; D019061:src-Family Kinases; D017027:Protein Tyrosine Phosphatases; D000077185:Resveratrol