[Influence of low doses of ionizing radiation on tenascin expression in hybridoma cell systems].
One of the achievements of the modern radiation ecology is the preparation and application of stable eukariotic cell lines to solve various problems occurring under exposure to ionizing radiation, especially to low doses. The detection of onco-fetal protein--tenascin in different embryonic and tumor cells of humans and animals supposes the probability of appropriate gene expression in lymphoid cells, including hybridomal cells. Using the immunochemical method, the study of tenascin expression in two mouse hybridomal lines was carried out. Tenascin was revealed in hybridomal lines MLC-1 and K-48. Further hybridomal cell lines were exposed to X-ray radiation (120 KV) with doses 2.10,15 cGy. The obtained results demonstrated the sensitivity of tenascin expression to low doses of ionizing radiation, that may be used as a convenient model of studying of genotoxic effects of various damaging ionizing agents on a cell level.
Lunga
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Research Center for Medical Genetics, Academy of Medical Sciences, Moscow, 115478 Russia. lounga@medgen.ru
D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression; D006801:Humans; D006825:Hybridomas / Q000378:metabolism* / Q000528:radiation effects*; D007120:Immunochemistry; D008214:Lymphocytes / Q000378:metabolism; D051379:Mice; D011829:Radiation Dosage; D011839:Radiation, Ionizing; D019063:Tenascin / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects*; D013997:Time Factors
D003470:Culture Media; D019063:Tenascin