title: [Expression and clinical implication of multidrug resistance gene and multidrug resistant-associated protein gene in patients with hypopharyngeal squamous cell carcinoma].
abstract: OBJECTIVE
  To study the relationship between the expression of MDR-1 mRNA/P-glycoprotein (P-gp). MRP mRNA and the clinical pathological characteristics of the patients with hypopharyngeal squamous cell carcinoma.
  
  
  METHODS
  Reverse transcription polymerase chain reaction(RT-PCR) assay was used to detect the expression of MDR-1 mRNA and MRP mRNA in 25 specimens of the untreated advanced hypopharyngeal carcinoma. Meantime, immunohistochemistry SABC technique was used to test P-gp in 60 tumor specimens.
  
  
  RESULTS
  The positive expression rates of MDR-1mRNA and P-gp in hypopharyngeal carcinoma were 48 percent (12/25) and 38.3 percent(23/60), respectively. There was a correlation between expression of MDR-1 mRNA and its product P-gp in 25 patients using RT-PCR assay(P < 0.05). The positive expression rate of MRP mRNA was 52 percent (13/25), which was significantly related to metastases of cervical lymph node (P < 0.01).
  
  
  CONCLUSIONS
  Forty-eight percent of the untreated patients with hypopharyngeal carcinoma presented positive expression of MDR-1 mRNA, which was correlated with its gene product P-gp. The expression of MRP mRNA was associated with increased tumor dissemination and metastases.
authors.affiliation: Department of Otorhinolaryngology, First Affiliated Hospital of Shandong Medical University, Jinan 250012.
authors.forename: X
authors.initials: X
authors.lastname: Cai
authors.forename: T
authors.initials: T
authors.lastname: Wang
authors.forename: L
authors.initials: L
authors.lastname: Shi
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000096:biosynthesis / Q000235:genetics; D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D019450:Genes, MDR / Q000235:genetics*; D006801:Humans; D007012:Hypopharyngeal Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D008297:Male; D008875:Middle Aged; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000235:genetics*; D009362:Neoplasm Metastasis; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D012333:RNA, Messenger