Effect of Emdogain on human periodontal fibroblasts in an in vitro wound-healing model.
OBJECTIVE
The aim of this study was to evaluate the influence of Emdogain (EMD) on cultured gingival fibroblasts, periodontal ligament fibroblasts and dermal fibroblasts, using an in vitro model of wound healing.


BACKGROUND
Enamel matrix derivative has been demonstrated to promote periodontal regeneration. However, the precise mechanisms by which this agent acts are still unclear.


METHODS
The effect of EMD on proliferation of the cells was studied using subconfluent cultures of gingival fibroblasts and periodontal ligament fibroblasts. The cells were made quiescent overnight and then stimulated with various concentrations of EMD (10, 50, 100 and 150 microg/ml) for 24 h. Negative and positive controls were cells cultured in media containing 0.2% and 10% fetal calf serum (FCS). The DNA synthesis was measured by the cellular uptake of [3H]thymidine. For in vitro wounding the cells were cultured, wounded and stimulated with 0.2% FCS, 10% FCS and EMD at a concentration of 20 microg/ml. The percentage of wound fill after treatment was measured after d 1, 4, 6, 12 and 16. The proliferation of cells was also calculated by the extent of incorporation of crystal violet.


RESULTS
The results demonstrated that cells in vitro fill an empty space by a combination of proliferation and cell migration. The most rapid closure of a wound area occurred where both proliferation and migration can occur as was seen when wounded cultures were maintained in 10% FCS or at a concentration of 20 microg/ml EMD which promoted proliferation.


CONCLUSIONS
Therefore, EMD appears to exert an influence on cells that is compatible with improved wound healing.
Rincon
J C
JC
Department of Dentistry, University of Queensland, Brisbane, Australia.
Haase
H R
HR
Bartold
P M
PM
D000704:Analysis of Variance; D001769:Blood; D018929:Cell Culture Techniques; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D003746:Dental Enamel Proteins / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D005881:Gingiva / Q000166:cytology / Q000187:drug effects; D006801:Humans; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects*; D012867:Skin / Q000166:cytology / Q000187:drug effects; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects
D003470:Culture Media; D003746:Dental Enamel Proteins; C109069:enamel matrix proteins; D004247:DNA