title: Reducing image distortions due to temperature-related microscope stage drift.
abstract: Distortions in confocal 3D image data sets were related to movements of the microscope stage that matched fluctuations in laboratory temperature. Movement was apparent in all three axes. Adding a draught-proof enclosure, covering the stage, objective lenses and supporting structures, minimized these short-term fluctuations. However, the stage still tracked slow changes in laboratory temperature and was sensitive to heat sources mounted on the microscope. Suggestions are made about microscope design.
authors.affiliation: Confocal and Image Analysis Laboratory, NIMR, The Ridgeway, Mill Hill, London NW7 1AA, UK.
authors.forename: J
authors.initials: J
authors.lastname: Adler
authors.forename: S N
authors.initials: SN
authors.lastname: Pagakis
mesh_terms: D007089:Image Enhancement; D007091:Image Processing, Computer-Assisted / Q000379:methods; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods; D009038:Motion; D013696:Temperature