title: Repair of UV damage in Halobacterium salinarum.
abstract: Halobacterium is one of the few known Archaea that tolerates high levels of sunlight in its natural environment. Photoreactivation is probably its most important strategy for surviving UV irradiation and we have shown that both of the major UV photoproducts, cyclobutane pyrimidine dimers (CPDs) and (6-4) photoproducts, can be very efficiently repaired by photoreactivation in this organism. There are two putative photolyase gene homologues in the published genome sequence of Halobacterium sp. NRC-1. We have made a mutant deleted in one of these, phr2, and confirmed that this gene codes for a CPD photolyase. (6-4) photoproducts are still photoreactivated in the mutant so we are currently establishing whether the other homologue, phr1, codes for a (6-4) photolyase. We have also demonstrated an excision repair capacity that operates in the absence of visible light but the nature of this pathway is not yet known. There is probably a bacteria-type excision-repair mechanism, since homologues of uvrA, uvrB, uvrC and uvrD have been identified in the Halobacterium genome. However, there are also homologues of eukaryotic nucleotide-excision-repair genes ( Saccharomyces cerevisiae RAD3, RAD25 and RAD2 ) so there may be multiple repair mechanisms for UV damage in Halobacterium.
authors.affiliation: School of Biological and Molecular Sciences, Oxford Brookes University, Gipsy Lane, Oxford OX3 0BP, UK. sjmccready@brookes.ac.uk
authors.forename: S
authors.initials: S
authors.lastname: McCready
authors.forename: L
authors.initials: L
authors.lastname: Marcello
mesh_terms: D000818:Animals; D004249:DNA Damage / Q000528:radiation effects*; D004260:DNA Repair / Q000235:genetics / Q000528:radiation effects*; D019641:DNA, Archaeal / Q000235:genetics / Q000528:radiation effects*; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000528:radiation effects; D019617:Halobacterium salinarum / Q000145:classification / Q000235:genetics* / Q000528:radiation effects; D010802:Phylogeny
chemical_list: D019641:DNA, Archaeal; D004255:Deoxyribodipyrimidine Photo-Lyase