Genomic organization and expression of mouse Tpt1 gene.
The translationally controlled tumor protein (TCTP), also known as histamine-releasing factor (HRF), is encoded by a gene (Tpt1) that is highly conserved throughout phylogeny. TCTP is implicated in cell growth, acute allergic response, and apoptosis. In the present study, seven putative Tpt1 genes with different chromosomal localizations were identified in the mouse genome. In six of them, analysis of the 5' and 3' untranslated regions revealed the presence of flanking direct repeats and residual poly(A) tails typical of pseudogenes. Only three of the seven genes can produce a protein of the expected molecular weight. We isolated the genomic DNA of these three genes to analyze their sequence, genomic organization, and in vitro promoter activity. We found that mouse Tpt1 is localized on chromosome 14 with a canonical intron-exon organization, a functional promoter, and only one transcript that is ubiquitously expressed in all tissues.
Fiucci
Giusy
G
Molecular Engines Laboratories, 20 Rue Bouvier, 75011 Paris, France. gfiucci@mail.molecular-engines.com
Lespagnol
Alexandra
A
Stumptner-Cuvelette
Pamela
P
Beaucourt
Séverine
S
Duflaut
Dominique
D
Susini
Laurent
L
Amson
Robert
R
Telerman
Adam
A
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000235:genetics*; D002875:Chromosomes; D040461:Gene Components; D016678:Genome; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics* / Q000378:metabolism; D011061:Poly A; D011401:Promoter Regions, Genetic; D011544:Pseudogenes; D012333:RNA, Messenger; D012091:Repetitive Sequences, Nucleic Acid; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D014018:Tissue Distribution; D000090324:Tumor Protein, Translationally-Controlled 1
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; C000715648:Tpt1 protein, mouse; D000090324:Tumor Protein, Translationally-Controlled 1; D011061:Poly A; D017853:Phosphotransferases (Alcohol Group Acceptor); C106173:TPT1 protein, S cerevisiae