title: [Determination of stilbene derivative in shengfa powders by HPLC].
abstract: OBJECTIVE
  To develop a new method for the determination of the idiosyncratic component, 2,3,5,4'-tetrahydroxy-stilbene-2-O-beta-D-glycoside in Shengfa Powders.
  
  
  METHODS
  A HPLC method was set up, using Hypersil BDS C18 column (5 microns, 4.6 mm x 150 mm), acetonitrile-water(18:82) as mobile phase, with detection at 320 nm.
  
  
  RESULTS
  The calibration curve was linear in the range of 0.45-2.25 micrograms, r = 0.9998, the average recovery was 99.5%, RSD = 1.2%(n = 6).
  
  
  CONCLUSIONS
  The active constituent 2,3,5,4'-tetrahydroxy-stilbene-2-O-beta-D-glycoside in Shengfa Powders can be separated effectively. This method is simple, specific and exact.
authors.affiliation: Department of Pharmacy, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, Shanxi, China. bqhu@21cn.com
authors.forename: Ben-quan
authors.initials: BQ
authors.lastname: Hu
authors.forename: Ya-jun
authors.initials: YJ
authors.lastname: ShI
authors.forename: Ping
authors.initials: P
authors.lastname: Huang
mesh_terms: D004338:Drug Combinations; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D006027:Glycosides / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D028502:Polygonum / Q000737:chemistry*; D011208:Powders / Q000737:chemistry; D011786:Quality Control; D013267:Stilbenes / Q000032:analysis*
chemical_list: D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D006027:Glycosides; D011208:Powders; D013267:Stilbenes