title: Outer membrane protein A (OmpA) activates human epidermal Langerhans cells.
abstract: Outer membrane protein (Omp)A is highly represented and conserved in the Enterobacteriaceae family. Using a recombinant OmpA from Klebsiella pneumoniae (kpOmpA), we have analysed the interaction between this bacterial cell wall protein and human Langerhans cells (LC), the antigen-presenting cells of the epidermis and mucosa. We showed that biotinylated kpOmpA binds to human LC freshly isolated from epidermis. kpOmpA up-regulated MHC class II, CD86 and CCR7 expression, enhanced migration in response to macrophage inflammatory protein-3beta (MIP-3beta) through a reconstituted basement membrane mimicking the prerequisite passage through the dermal-epidermal basement membrane on the way to lymph nodes. The allostimulatory function of kpOmpA-treated LC was more potent than that of untreated cells. Even though the proportion of LC which binds kpOmpA was shown to vary between individuals, our data indicate that kpOmpA binds to and activates LC, and suggest that recognition of OmpA by LC may be an initiating event in the antibacterial host response.
authors.affiliation: INSERM U346, affilié CNRS, Hôpital E. Herriot, Lyon, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Godefroy
authors.forename: Nathalie
authors.initials: N
authors.lastname: Corvaia
authors.forename: Daniel
authors.initials: D
authors.lastname: Schmitt
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Aubry
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Bonnefoy
authors.forename: Pascale
authors.initials: P
authors.lastname: Jeannin
authors.forename: Marie-Jeanne
authors.initials: MJ
authors.lastname: Staquet
mesh_terms: D015703:Antigens, CD / Q000096:biosynthesis; D018949:Antigens, CD1 / Q000096:biosynthesis; D051940:B7-2 Antigen; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism* / Q000494:pharmacology; D002455:Cell Division / Q000276:immunology; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000096:biosynthesis; D006801:Humans; D016130:Immunophenotyping; D007801:Langerhans Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D011485:Protein Binding; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D015703:Antigens, CD; D018949:Antigens, CD1; D051940:B7-2 Antigen; D001425:Bacterial Outer Membrane Proteins; C419393:CD1a antigen; C497964:CD86 protein, human; D006684:HLA-DR Antigens; D008562:Membrane Glycoproteins