An in vitro uniaxial stretch model for axonal injury.
We have developed a unique uniaxial stretching device to study axonal injury and neural cell death resulting from brain tissue deformations common in traumatic head injuries. Using displacement control rather than force control, this device is capable of achieving strains >70% and strain rates up to 90 s(-1), well above those currently used for studying axonal injury. We have demonstrated that the deformation of the specimen was uniaxial, uniform and highly reproducible; the prespecified displacement profiles could be realized almost precisely; and adequate cell adhesion could be achieved readily. The entire device can fit into a biological safety cabinet to maintain sterility, and the specimens are convenient for cell culture. This device can be used to investigate a wide range of biomechanical issues involved in diffuse axonal injury.
Pfister
Bryan J
BJ
Department of Neurosurgery, University of Pennsylvania, Philadelphia, PA, USA.
Weihs
Timothy P
TP
Betenbaugh
Michael
M
Bao
Gang
G
D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001369:Axons / Q000473:pathology* / Q000502:physiology*; D001930:Brain Injuries / Q000473:pathology / Q000503:physiopathology; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D002460:Cell Line; D004548:Elasticity; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005910:Glioma / Q000473:pathology / Q000503:physiopathology; D008846:Micromanipulation / Q000295:instrumentation* / Q000379:methods; D008954:Models, Biological; D009038:Motion; D009447:Neuroblastoma / Q000473:pathology / Q000503:physiopathology; D009474:Neurons / Q000473:pathology / Q000502:physiology; D010812:Physical Stimulation / Q000295:instrumentation* / Q000379:methods; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013314:Stress, Mechanical