title: Regulation of hyphal growth and sporulation of the insect pathogenic fungus Entomophthora thripidum in vitro.
abstract: Entomophthora thripidum is an obligate biotrophic insect pathogenic fungus that grows as protoplasts within the hemocoel of thrips. Prior to penetration through the insect cuticle and spore formation at the insect surface the protoplasts switch to hyphal growth. In vitro, the differentiation to hyphal growth was a prerequisite for the subsequent formation of infectious spores and was detected 10-20 days after inoculation. E. thripidum secreted a factor that autoinduced the differentiation to hyphal growth. The discovery of this activity inducing hyphal growth made possible the reliable production of spores, the infection of host insects and the consecutive re-isolation of the fungus from the infected insects.
authors.affiliation: Institute for Microbiology, ETH Zürich, Schmelzbergstrasse 7, 8092, Zurich, Switzerland. florian.freimoser@ipw.biol.etzh.ch
authors.forename: Florian M
authors.initials: FM
authors.lastname: Freimoser
authors.forename: Anne
authors.initials: A
authors.lastname: Grundschober
authors.forename: Urs
authors.initials: U
authors.lastname: Tuor
authors.forename: Markus
authors.initials: M
authors.lastname: Aebi
mesh_terms: D000818:Animals; D004773:Entomophthora / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure; D025301:Hyphae / Q000502:physiology*; D066298:In Vitro Techniques; D007313:Insecta / Q000382:microbiology; D025282:Mycelium / Q000502:physiology; D009584:Nitrogen / Q000378:metabolism; D013172:Spores, Fungal / Q000502:physiology*
chemical_list: D009584:Nitrogen