title: Prolin-rich tyrosine kinase 2 (PYK2) expression and localization in mouse testis.
abstract: Prolin-rich kinase 2 (PYK2) is a nonreceptor tyrosine kinase related to the focal adhesion kinase (FAK) p125(FAK). PYK2 is rapidly phosphorylated on tyrosine residues in response to various stimuli, such as tumor necrosis factor-alpha (TNF-alpha), changes in osmolarity, elevation in intracellular calcium concentration, angiotensin, and UV irradiation. PYK2 has ligand sequences for Src homology 2 and 3 (SH-2 and SH-3), and has binding sites for paxillin and p130(cas). Activation of PYK2 leads to modulation of ion channel function, phosphorylation of tyrosine residues, and activation of the MAP kinase signaling pathways. Immunocytochemistry shows that PYK2 is present in mouse germinal and Sertoli cells (ser). Northern blot and immunoprecipitation analysis demonstrate that, among germinal cells, PYK2 is more abundant in spermatocytes (spc) and spermatids (spt); in addition, immunofluorescence analysis clearly shows that the diffuse cytoplasmic localization of PYK2 changes in a specific cellular compartment in spt and spermatozoa.
authors.affiliation: Dipartimento di Medicina Sperimentale, II Università di Napoli, Naples, Italy. Paolo.Chieffi@unina2.it
authors.forename: Paolo
authors.initials: P
authors.lastname: Chieffi
authors.forename: Marco
authors.initials: M
authors.lastname: Barchi
authors.forename: Silvia
authors.initials: S
authors.lastname: Di Agostino
authors.forename: Pellegrino
authors.initials: P
authors.lastname: Rossi
authors.forename: Donatella
authors.initials: D
authors.lastname: Tramontano
authors.forename: Raffaele
authors.initials: R
authors.lastname: Geremia
mesh_terms: D000818:Animals; D003593:Cytoplasm / Q000201:enzymology; D004789:Enzyme Activation; D051418:Focal Adhesion Kinase 2; D015971:Gene Expression Regulation, Enzymologic; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D011505:Protein-Tyrosine Kinases / Q000235:genetics* / Q000378:metabolism*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D013075:Sperm Capacitation / Q000502:physiology*; D013087:Spermatids / Q000201:enzymology; D013090:Spermatocytes / Q000201:enzymology; D013737:Testis / Q000201:enzymology*
chemical_list: D012313:RNA; D011505:Protein-Tyrosine Kinases; D051418:Focal Adhesion Kinase 2; C496053:Ptk2b protein, mouse