Establishing the protein MIA (melanoma inhibitory activity) as a marker for chondrocyte differentiation.
Melanoma inhibitory activity (MIA), also referred to as cartilage derived retinoic acid-sensitive protein (CD-RAP), is detected physiologically in cartilage tissue and pathologically in malignant melanomas. To measure MIA/CD-RAP quantitatively we developed a sensitive ELISA system. Recently, we described diagnostic applications of the MIA-ELISA in patients with cartilage diseases. The study described herein was performed to determine whether there is any relation between MIA/CD-RAP levels and the degree of chondrocyte differentiation in tissue culture and to analyse whether MIA/CD-RAP may serve as a useful marker to control chondrocyte differentiation in in vitro tissue engineering. Our data provide evidence that measuring MIA in tissue culture supernatant by a quantitative ELISA can be used as a marker for differentiated chondrocytes.
Bosserhoff
Anja K
AK
Institutes of Pathology, University of Regensburg, Franz-Josef-Strauss-Allee 11, Germany. anja.bosserhoff@klinik.uni-regensburg.de
Buettner
Reinhard
R
D015415:Biomarkers; D002356:Cartilage / Q000378:metabolism; D002357:Cartilage Diseases / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D016326:Extracellular Matrix Proteins; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D009363:Neoplasm Proteins; D014176:Protein Biosynthesis; D011506:Proteins / Q000502:physiology*; D012313:RNA / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology; D013997:Time Factors; D023822:Tissue Engineering
D015415:Biomarkers; D016326:Extracellular Matrix Proteins; C090059:MIA protein, human; D009363:Neoplasm Proteins; D011506:Proteins; D012313:RNA