Cell fractionation of parasitic protozoa: a review.
Cell fractionation, a methodological strategy for obtaining purified organelle preparations, has been applied successfully to parasitic protozoa by a number of investigators. Here we present and discuss the work of several groups that have obtained highly purified subcellular fractions from trypanosomatids, Apicomplexa and trichomonads, and whose work have added substantially to our knowledge of the cell biology of these parasites.
de Souza
Wanderley
W
Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-900, Brasil. wsouza@biof.ufrj.br
da Cunha-e-Silva
Narcisa Leal
NL
D000818:Animals; D016782:Apicomplexa / Q000648:ultrastructure*; D002458:Cell Fractionation / Q000379:methods*; D002462:Cell Membrane / Q000648:ultrastructure; D008854:Microscopy, Electron; D015388:Organelles / Q000648:ultrastructure*; D014244:Trichomonas / Q000648:ultrastructure*; D014351:Trypanosomatina / Q000648:ultrastructure*