[Study on the directed inducing process of cartilage cells differentiated from human marrow mesenchymal stem cells].
OBJECTIVE
To study the feasibility of constructing tissue engineered cartilage cells by differentiated human bone marrow mesenchymal stem cells(MSC) cultured in vitro.


METHODS
The MSC were isolated from the rib by density gradient centrifuge, then induced into chondrogenic differentiation by adding dexamethasone, transforming growth factor-beta 1 (TGF-beta 1), ascorbic acid, insulin and bovine serum albumin.


RESULTS
Some cells were degenerated and disappeared after cultured in vitro. But the residual cells were surviving and have secreted metachromatic matrix, which is positive with toluidine blue staining Collagen II antigens expression is positive, which is one of the cartilage cells characteristics.


CONCLUSIONS
MSC can be induced to cartilage cells in vitro, MSC can be used as functional cells for constructing tissue engineered cartilage.
Jiang
Xun
X
Department of Otolaryngology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China. Jiangxun@sohu.com
Cui
Pengcheng
P
Chen
Wenxian
W
Zhao
Daqing
D
Luo
Jiasheng
J
Li
Guize
G
Sun
An
A
D002454:Cell Differentiation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology*; D005240:Feasibility Studies; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D023822:Tissue Engineering