Oxygen-evolving enhancer protein 2 is phosphorylated by glycine-rich protein 3/wall-associated kinase 1 in Arabidopsis.
The Arabidopsis wall-associated receptor kinase, WAK1, is a member of WAK family that links the plasma membrane to the extracellular matrix. A glycine-rich secreted protein, AtGRP-3, was previously shown to regulate WAK1 functions through binding to the extracellular domain of WAK1. In this study, we sought to determine the downstream molecules of the AtGRP-3/WAK1 signaling pathway, by using two-dimensional gel electrophoresis combined with Edman sequencing and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS). We report here that a chloroplast protein, oxygen-evolving enhancer protein 2 (OEE2), specifically interacts with the cytoplasmic kinase domain of WAK1 and becomes phosphorylated in an AtGRP-3-dependent manner. The phosphorylation of OEE2 is also induced in Arabidopsis by treatment with avirulent Pseudomonas syringae. Taken together, these results suggest that OEE2 activity is regulated by AtGRP-3/WAK1.
Yang
Eun Ju
EJ
Kumho Life and Environmental Science Laboratory, 1 Oryong-dong, Buk-gu, Gwangju 500-712, Republic of Korea.
Oh
Young Ah
YA
Lee
Eui Seung
ES
Park
Ae Ran
AR
Cho
Somi K
SK
Yoo
Yung Joon
YJ
Park
Ohkmae K
OK
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology* / Q000378:metabolism / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D020543:Proteome / Q000032:analysis; D011523:Protoplasts / Q000201:enzymology; D011549:Pseudomonas / Q000472:pathogenicity
D029681:Arabidopsis Proteins; C432244:GRP-3 protein, Arabidopsis; D008565:Membrane Proteins; C475688:OEE2 protein, Arabidopsis; D020543:Proteome; D011494:Protein Kinases; C101093:WAK1 protein, Arabidopsis