A comparative study of biochemical and immunological properties of triosephosphate isomerase from Taenia solium and Sus scrofa.
We produced the Taenia solium triosephosphate isomerase (TPI) in Escherichia coli and compared its biochemical and immunological properties with those of the commercial TPI from Sus scrofa. Taenia solium TPI is a homodimer composed of two 27-kDa monomers, with a specific activity of 5,683 U/mg and a Km value of 0.758, and S. scrofa TPI is also dimeric with similar monomeric molecular weight, specific activity of 4,227 U/mg, and a Km value of 0.51. The catalytic parameters for the isomerization of glyceraldehyde 3-phosphate, affinity between TPI monomers, and kinetic thermal denaturation and inactivation were similar for both enzymes. Anti-T. solium TPI antibodies cross-react weakly with Schistosoma mansoni TPI but do not cross-react with S. scrofa, human, or protozoan TPIs. These antibodies inhibited T. solium TPI activity but did not affect S. scrofa enzymatic activity. Immunizations with 1 microg of the T. solium TPI reduced 52% of cysticerci in a mouse-Taenia crassiceps model 1 mo after challenge. Our findings show that T. solium and S. scrofa TPIs possess similar biochemical and enzymatic properties but do not share immunological properties because anti-T. solium TPI antibodies did not recognize S. scrofa TPI. Inhibition of enzyme activity by anti-TPI antibodies suggests that they can be used as inhibitors of the enzyme.
Jiménez
Lucía
L
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Edificio A, 2 Piso, Ciudad Universitaria, México DF 04510, México.
Fernández-Velasco
D Alejandro
DA
Willms
Kaethe
K
Landa
Abraham
A
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D000947:Antigens, Helminth / Q000737:chemistry / Q000276:immunology; D015153:Blotting, Western; D002850:Chromatography, Gel; D002942:Circular Dichroism; D003551:Cysticercosis / Q000276:immunology / Q000517:prevention & control; D004195:Disease Models, Animal; D004591:Electrophoresis, Polyacrylamide Gel; D015801:Helminth Proteins / Q000737:chemistry / Q000276:immunology; D006358:Hot Temperature; D007106:Immune Sera / Q000276:immunology; D051379:Mice; D017510:Protein Folding; D011817:Rabbits; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D013552:Swine / Q000378:metabolism*; D041201:Taenia solium / Q000201:enzymology*; D014305:Triose-Phosphate Isomerase / Q000737:chemistry* / Q000276:immunology* / Q000302:isolation & purification
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015801:Helminth Proteins; D007106:Immune Sera; D011994:Recombinant Proteins; D014305:Triose-Phosphate Isomerase