Cloning and characterization of a blood coagulation factor IX-binding protein from the venom of Trimeresurus stejnegeri.
A blood coagulation factor IX-binding protein (TSV-FIX-BP) was isolated from the snake venom of Trimeresurus stejnegeri. On SDS-polyacrylamide gel electrophoresis, TSV-FIX-BP showed a single band with an apparent molecular weight of 23,000 under non-reducing conditions, and two distinct bands with apparent molecular weights of 14,800 and 14,000 under reducing conditions. cDNA clones containing the coding sequences of TSV-FIX-BP were isolated and sequenced to determine the structure of the precursors of TSV-FIX-BP subunits. The deduced amino acid sequences of two subunits of TSV-FIX-BP were confirmed by N-terminal protein sequencing and trypsin-digested peptide mass fingerprinting. TSV-FIX-BP was a non-enzymatic C-type lectin-like anti-coagulant. The anti-coagulant activity of TSV-FIX-BP was mainly caused by its dose dependent interaction with blood coagulation factor IX but not with blood coagulation factor X.
Lee
Wen-Hui
WH
Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming, Yunnan 650223, People's Republic of China.
Zhuang
Qing-Ying
QY
Zhang
Yun
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000633:toxicity*; D003001:Cloning, Molecular; D003435:Crotalid Venoms / Q000737:chemistry / Q000235:genetics* / Q000633:toxicity*; D018076:DNA, Complementary / Q000235:genetics*; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D005164:Factor IX / Q000187:drug effects*; D015723:Gene Library; D008969:Molecular Sequence Data; D030162:Reptilian Proteins; D016415:Sequence Alignment; D017385:Sequence Homology; D017841:Trimeresurus
D002352:Carrier Proteins; D003435:Crotalid Venoms; D018076:DNA, Complementary; D030162:Reptilian Proteins; C029748:Trimeresurus venoms; C109516:factor IX-binding protein, Trimeresurus flavoviridis; D005164:Factor IX