Evaluation of hydralazine and procainamide effects on fibroblast membrane fluidity.
In this study the membrane fluidity of fibroblasts under different pharmacological treatment was investigated. Two drugs, hydralazine and procainamide, were used to treat the immortalized mouse NIH 3T3 and hamster B14 fibroblasts. Membrane lipid dynamics was measured by fluorescence spectroscopy and electron spin resonance techniques. Two kinds of fluorescent probes (TMA-DPH and 12-(9-anthroyloxy)-stearic acid (12-AS)) and two spin labels (5-doxylstearic acid (5-DS) and 12-doxylstearic acid (12-DS)) were used to monitor fluidity in the upper polar and in the hydrophobic core regions of the lipid bilayer. The drugs influenced the membrane hydrophobic core, of which hydralazine induced fluidization and procainamide increased the rigidity. The membrane fluidity at the surface of the lipid bilayer was not modified by the drugs which indicates that both drugs intercalated mainly into the inner core of the cell membrane.
Weglarz
Ludmiła
L
Department of Molecular Biology, Biochemistry and Biopharmacy, Medical University of Silesia, Narcyzów 1, 41-200, Sosnowiec, Poland. lweglarz@slam.katowice.pl
Koceva-Chyła
Aneta
A
Gwoździński
Krzysztof
K
Dzierzewicz
Zofia
Z
Jóźwiak
Zofia
Z
D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000494:pharmacology*; D000959:Antihypertensive Agents / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects; D002478:Cells, Cultured; D006224:Cricetinae; D004578:Electron Spin Resonance Spectroscopy; D005347:Fibroblasts / Q000187:drug effects; D006830:Hydralazine / Q000494:pharmacology*; D008560:Membrane Fluidity / Q000187:drug effects*; D051379:Mice; D041681:NIH 3T3 Cells; D011342:Procainamide / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence
D000889:Anti-Arrhythmia Agents; D000959:Antihypertensive Agents; D006830:Hydralazine; D011342:Procainamide