title: Globulins enhance in vitro iron but not zinc dialysability: a study on six legume species.
abstract: The study was addressed to evaluate the in vitro iron and zinc dialysability from the globulin fraction of six legumes. Five legume species including white bean, mottled bean (Taylor bean), chickpea, lentil, lupin, and a modified mottled bean variety, selected by back-crossing to obtain seeds with globulins composed by G1 fraction only, were used. Globulins (G1 + G2) were extracted from the seeds and analysed for their in vitro iron and zinc dialysability. The highest globulin concentration was detected in lentil (89%). The percentage of globulins in the modified variety of Taylor bean (G1 only) was higher than that of the commercial variety (G1 + G2). The highest concentration of iron was found in Taylor bean globulins. The modified variety of Taylor bean contained 2.6-fold higher iron concentration than the whole seed, and the commercial variety had 1.8-fold higher iron only. The highest zinc concentration was found in lentil globulins. Also iron dialysability from globulins was markedly higher than that of the respective whole seed. The highest value of iron dialysability was found in lentil (10.8%). Zinc dialysability was generally high (above 20%), but no significant differences between whole seed and globulins were detected. The results showed that globulins enhanced iron but not zinc dialysability. Lupin and the modified variety of Taylor bean showed a different behaviour in terms of mineral dialysability compared to the other legumes. The amino acid composition of the digestion products of whole seeds and globulins failed to evidence any direct influence on iron and zinc availability.
authors.affiliation: Istituto Nazionale di Ricerca per gli Alimenti e La Nutrizione, Rome, Italy. lombardiboccia@inran.it
authors.forename: Ginevra
authors.initials: G
authors.lastname: Lombardi-Boccia
authors.forename: Stefania
authors.initials: S
authors.lastname: Ruggeri
authors.forename: Altero
authors.initials: A
authors.lastname: Aguzzi
authors.forename: Marsilio
authors.initials: M
authors.lastname: Cappelloni
mesh_terms: D000596:Amino Acids / Q000032:analysis; D007887:Fabaceae / Q000378:metabolism*; D005916:Globulins / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry; D013045:Species Specificity; D014131:Trace Elements / Q000032:analysis; D015032:Zinc / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D005916:Globulins; D010455:Peptides; D014131:Trace Elements; D007501:Iron; D015032:Zinc