[Tissue-engineered auricled cartilage: an experimental study].
OBJECTIVE
To study the feasibility of engineering auricled neocartilage with chitosan/polylacticacid-polycrylactone (PLA-PCL) network scaffolds and to search the difference between dynamic and silent chondrocytal culture techniques.


METHODS
Chondrocytes from auricled cartilage of 4 weeks old New Zealand White rabbit were seeded onto chitosan/PLA-PCL network. Ten cell-polymer scaffolds were divided into two groups: dynamic group (n1 = 5) with rotating bioreactors and silent group (n2 = 5) with ordinary dishes to culture. Using scan electroscope, grossly histological and immunohistological techniques, the morphological evaluation was done individually at 1st week in vitro, 4th and 8th week in vivo.


RESULTS
Chondrocytes adhered and grew up well on the network, but more quantities of chondrocytes, Glycoaminoglygan (GAG) and type II collagen were found in dynamic group. There was an obvious difference between dynamic and silent group (P < 0.05).


CONCLUSIONS
Chitosan/PLA-PCL network scaffold is good for adhesions and growth of chondrocytes. Furthermore, dynamic cell culture method is better than silent method for formation of neocartilage.
Zhang
Ruilin
R
Department of Otorhinolaryngology, West China Hospital, Sichuan University, Chengdu 610041, China. ent2000@mcwcums.com
Liang
Chuanyu
C
Qin
Tingwu
T
Yang
Zhiming
Z
Liu
Jun
J
Li
Xiuqun
X
Li
Wen
W
D000818:Animals; D002686:Chitin / Q000031:analogs & derivatives*; D048271:Chitosan; D019902:Chondrocytes / Q000502:physiology*; D020219:Chondrogenesis; D024043:Collagen Type II; D046508:Culture Techniques; D004425:Ear Cartilage; D011817:Rabbits
D024043:Collagen Type II; D002686:Chitin; D048271:Chitosan