Combined solvent and water activity stresses on turgor regulation and membrane adaptation in Oceanimonas baumannii ATCC 700832.
Oceanimonas baumannii ATCC 700832 is a Gram negative marine bacterium capable of utilising phenol as asole carbon source. The ability of the bacterium to tolerate low water activity when utilising either succinate or phenol as a substrate in minimal medium was studied. The membrane lipid and protein composition showed two discreet adaptive phases as salinity increased. Firstly, when NaCl concentration was increased from 0.15% (w/v), the minimum at which growth was observed, to 1% NaCl (w/v), the ratio of zwitterionic to anionic phospholipids in the membrane increased significantly. At the same time the ratio of saturated to unsaturated fatty acids and the total membrane protein decreased significantly. The second phase was observed when salinity was increased from 1% to 7% NaCl (w/v) as the ratio of zwitterionic to anionic phospholipids decreased and membrane protein increased. However, the ratio of saturated to unsaturated fatty acids was unaffected. Salinity also affected the tolerance of cultures to elevated levels of phenol. Cultures grown in 0.15% NaCl (w/v) could tolerate 12 mM phenol, whereas in the presence of 1% NaCl (w/v) cultures continued to grow in up to 20 mM phenol and in 7% NaCl (w/v) cultures 8 mM phenol could be tolerated. Changes to the composition of the membrane phospholipids and fatty acids were also observed when phenol concentrations were at the maximum that could be tolerated. Under such conditions the ratio of zwitterionic to anionic phospholipids decreased twofold compared to cultures utilising 4 mM phenol as the substrate, in all salinities except in 7% NaCl (w/v) cultures, where there was no significant effect. The ratio of saturated to unsaturated fatty acids increased significantly in all salinities compared to cultures grown with 4 mM phenol.
Brown
Geoffrey R
GR
The School of Sciences, University of Sunderland, Sunderland SR1 3SD, UK.
Sutcliffe
Iain C
IC
Cummings
Stephen P
SP
D000222:Adaptation, Physiological; D044044:Aeromonadaceae / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D008563:Membrane Lipids / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D010636:Phenols / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D012623:Seawater; D012965:Sodium Chloride / Q000494:pharmacology; D012997:Solvents / Q000737:chemistry*; D019802:Succinic Acid / Q000378:metabolism; D014867:Water / Q000737:chemistry*
D005227:Fatty Acids; D008563:Membrane Lipids; D008565:Membrane Proteins; D010636:Phenols; D010743:Phospholipids; D012997:Solvents; D014867:Water; D012965:Sodium Chloride; D019802:Succinic Acid