Mapping of gene-specific markers on the genetic map of chickpea (Cicer arietinum L.).
With the exception of the fact that it is made up of eight different chromosomes, the physical organization of the 738-Mb genome of the important legume crop chickpea (Cicer arietinum L.) is unknown. In an attempt to increase our knowledge of the basic structure of this genome, we determined the map positions of a series of genes involved in plant defence responses (DR) by genetic linkage analysis. Exploiting the sequence data available in GenBank, we selected genes known to be induced in chickpea and other plants by pathogen attack. Gene-specific primers were designed based on conserved regions, and used to detect the corresponding gene sequences in a segregating population derived from an interspecific cross between Cicer arietinum and C. reticulatum. Forty-seven gene-specific markers were integrated into an existing map based on STMS, AFLP, DAF and other anonymous markers. The potential of this approach is discussed.
Pfaff
T
T
Plant Molecular Biology, Biocenter, University of Frankfurt, Marie-Curie-Str. 9, Germany.
Kahl
G
G
D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D019992:Databases as Topic; D005091:Exons; D007887:Fabaceae / Q000235:genetics*; D008040:Genetic Linkage; D005819:Genetic Markers; D018745:Genome, Plant; D008957:Models, Genetic; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D018626:Retroelements
D017931:DNA Primers; D005819:Genetic Markers; D018626:Retroelements; D004247:DNA