The role of organic solvents in the separation of nonionic compounds by capillary electrophoresis.
Although nonionic compounds can be separated by micellar electrokinetic chromatography (MEKC), application of this technique is restricted by a somewhat limited elution range. Incorporation of organic solvents in the background electrolyte (BGE) greatly extends the scope of MEKC and provides a major variable in optimizing the separation of neutral analytes. This paper provides a systematic review of the principles and scope of the separation of neutral analytes by capillary electrophoresis (CE) in organic-aqueous solution. The methods surveyed include those that use tetraalkylammonium salts, dioctyl sulfosuccinate, lauryl poly(oxyethylene) sulfate. Polyaromatic hydrocarbon (PAH) compounds can be separated using sodium hexadecyl sulfate in 70% methanol (30% aqueous) to 100% methanol.
Fritz
James S
JS
Department of Chemistry, Iowa State University, Ames, IA 50011, USA. kniss@ameslab.gov
D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D004143:Dioctyl Sulfosuccinic Acid / Q000302:isolation & purification*; D011092:Polyethylene Glycols / Q000302:isolation & purification*; D000644:Quaternary Ammonium Compounds / Q000302:isolation & purification*; D012967:Sodium Dodecyl Sulfate / Q000302:isolation & purification*; D012997:Solvents / Q000737:chemistry*
D000644:Quaternary Ammonium Compounds; D012997:Solvents; C482380:lauryl poly(oxyethylene)sulfate; D004143:Dioctyl Sulfosuccinic Acid; D012967:Sodium Dodecyl Sulfate; D011092:Polyethylene Glycols