title: Enzymes encoded by the farnesyl diphosphate synthase gene family in the Big Sagebrush Artemisia tridentata ssp. spiciformis.
abstract: Farnesyl diphosphate synthase catalyzes the sequential head-to-tail condensation of two molecules of isopentenyl diphosphate with dimethylallyl diphosphate. In plants the presence of farnesyl diphosphate synthase isozymes offers the possibility of differential regulation. Three full-length cDNAs encoding putative isoprenoid synthases, FDS-1, FDS-2, and FDS-5, with greater than 89% similarity were isolated from a Big Sagebrush Artemisia tridentata cDNA library using a three-step polymerase chain reaction protocol. One of the open reading frames, FDS-5, encoded a protein with an N-terminal amino acid extension that was identified as a plastidial targeting peptide. Recombinant histidine-tagged versions of three proteins were purified, and their enzymatic properties were characterized. FDS-1 and FDS-2 synthesized farnesyl diphosphate as the final chain elongation product, but their kinetic behavior varied. FDS-1 prefers geranyl diphosphate over dimethylallyl diphosphate as an allylic substrate and is active at acidic pH values compared with FDS-2. In contrast, FDS-5 synthesized two irregular monoterpenoids, chrysanthemyl diphosphate and lavandulyl diphosphate, when incubated with dimethylallyl diphosphate and an additional product, the regular monoterpene geranyl diphosphate, when incubated with isopentenyl diphosphate and dimethylallyl diphosphate. Specific cellular functions are proposed for each of the three enzymes, and a scenario for evolution of isoprenyl synthases in plants is presented.
authors.affiliation: Department of Chemistry, University of Utah, Salt Lake City, Utah 84112-0850, USA.
authors.forename: Andrea
authors.initials: A
authors.lastname: Hemmerlin
authors.forename: Susan B
authors.initials: SB
authors.lastname: Rivera
authors.forename: Hans K
authors.initials: HK
authors.lastname: Erickson
authors.forename: C Dale
authors.initials: CD
authors.lastname: Poulter
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D019306:Artemisia / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D018076:DNA, Complementary; D051228:Geranyltranstransferase; D008969:Molecular Sequence Data; D005810:Multigene Family; D009838:Oligodeoxyribonucleotides; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D009838:Oligodeoxyribonucleotides; D019883:Alkyl and Aryl Transferases; D051228:Geranyltranstransferase