Pig whey acidic protein gene is surrounded by two ubiquitously expressed genes.
A 140-kb pig DNA fragment containing the whey acidic protein (WAP) gene cloned in a bacterial artificial chromosome (BAC344H5) has been shown to contain all of the cis-elements necessary for position-independent, copy-dependent and tissue-specific expression in transgenic mice. The insert from this BAC was sequenced. This revealed the presence of two other genes with quite different expression patterns in pig tissues and in transfected HC11 mouse mammary cells. The RAMP3 gene is located 15 kb upstream of the WAP gene in reverse orientation. The CPR2 gene is located 5 kb downstream of the WAP gene in the same orientation. The same locus organization was found in the human genome. The region between RAMP3 and CPR2 in the human genome contains a WAP gene-like sequence with several points of mutation which may account for the absence of WAP from human milk.
Rival-Gervier
Sylvie
S
Laboratoire de biologie du développement et reproduction, Institut National de la Recherche Agronomique, INRA, 78350 Jouy-en-Josas, France. rival@jouy.inra.fr
Thépot
Dominique
D
Jolivet
Geneviève
G
Houdebine
Louis-Marie
LM
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D005786:Gene Expression Regulation; D008040:Genetic Linkage; D016678:Genome; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins / Q000235:genetics; D008894:Milk Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D058264:Receptor Activity-Modifying Protein 3; D058261:Receptor Activity-Modifying Proteins; D017422:Sequence Analysis, DNA; D013552:Swine; D026801:Synteny
D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D008894:Milk Proteins; C545504:RAMP3 protein, human; C545507:Ramp3 protein, mouse; D058264:Receptor Activity-Modifying Protein 3; D058261:Receptor Activity-Modifying Proteins; C024471:whey acidic proteins