[Detection of bcl-2/JH gene rearrangement by semi-nested polymerase chain reaction from fresh tumor samples in patients with laryngeal squamous cell carcinomas].
OBJECTIVE
To evaluate the clinical implications of bcl-2/JH gene rearrangement in laryngeal squamous cell carcinomas (LSCC).


METHODS
Bcl-2/JH gene rearrangement analysis in fresh tumor samples was performed in 60 patients with LSCC by semi-nested polymerase chain reaction(PCR).


RESULTS
The results showed that bcl-2/JH gene rearrangement was found in 37 out of 60 patients. The breakpoint was located within the major breakpoint region(mbr) in 33 of the 60 patients and the remaining patients had bcl-2 translocation within the minor cluster region(mcr). The results of the study showed that the rearrangement rate of bcl-2 gene was not related to the grade of differentiation, clinical stage, and neck lymph-node metastasis (P > 0.05), and it was related to heavy smoking (P < 0.05).


CONCLUSIONS
Detection of bcl-2/JH gene rearrangement could reveal that bcl-2/JH fusion gene in LSCC is an important molecular biological marker and has a significant role in occurrence and development of LSCC.
Zhang
X
X
Department of Otorhinolaryngology, Forth Affiliated Hospital, West China University of Medical Sciences, Chengdu 610041, China. 200299@mail.guoli.com.cn
Liu
S
S
Tang
S
S
Lin
D
D
Liang
C
C
An
H
H
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D005260:Female; D015321:Gene Rearrangement; D019254:Genes, bcl-2 / Q000235:genetics*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction