title: [Primary culture and biologic characters of human laryngeal papillomavirus positive epithelial cells in children].
abstract: OBJECTIVE
  To investigate the biologic characters of HPV positive laryngeal epithelial cells in vitro.
  
  
  METHODS
  To draw a cell growth curve by observing the biologic characters of HPV positive cells' growth pattern, PCR and nuclear acid hybridization were applied before and after culture for confirming HPV positive cells DNA.
  
  
  RESULTS
  Primary HPV positive cells often remain viable in culture for more than six weeks, there have HPV DNA before and after culture. We found that in these cells had common characters: lag phase, growth phase and plateau phase. The cells culture showed that the first to forth day there have many cells emigration from tissue pieces. The fifth to Seventh day was latent phase, it was mainly adhere growth in this period, proliferation of culture cells wasn't active. The eighth to eighteenth day was growth phase, cells number was increased quickly, speed of growth was faster. Then cells growth entered to plateau phase, cells number was increased slowly, cells showed vacuoles obviously. It indicated that these cells came to die.
  
  
  CONCLUSIONS
  Cells derives from HPV positive laryngeal epithelial have been successfully cultured, but to establish an animal model for HPV infection is difficult.
authors.affiliation: Department of Otorhinolaryngology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China. Jiangxun@sohu.com
authors.forename: Xun
authors.initials: X
authors.lastname: Jiang
authors.forename: Wenxian
authors.initials: W
authors.lastname: Chen
authors.forename: Zhipei
authors.initials: Z
authors.lastname: Zhang
authors.forename: Dianzhong
authors.initials: D
authors.lastname: Zhang
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Cui
authors.forename: Guize
authors.initials: G
authors.lastname: Li
authors.forename: Pengfei
authors.initials: P
authors.lastname: Gao
authors.forename: An
authors.initials: A
authors.lastname: Sun
mesh_terms: D002648:Child; D002675:Child, Preschool; D015344:DNA Probes, HPV; D004847:Epithelial Cells / Q000821:virology; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology* / Q000821:virology; D008297:Male; D010212:Papilloma / Q000473:pathology* / Q000821:virology; D027383:Papillomaviridae; D030361:Papillomavirus Infections / Q000473:pathology* / Q000821:virology; D014407:Tumor Cells, Cultured
chemical_list: D015344:DNA Probes, HPV