title: Analysis of gene expression in peripheral blood eosinophils from patients with atopic dermatitis by differential display.
abstract: To identify the genes related to atopic dermatitis (AD), we compared gene expression in eosinophils from AD patients and healthy volunteers. RNA was prepared from peripheral blood eosinophils. Gene expression was monitored by fluorescent differential display (DD) and real-time RT-PCR. Eighteen new sequences, including expressed sequence tags (ESTs), were expressed at higher levels in eosinophils from AD patients than in those from healthy volunteers. The functions of most of these genes are unknown. We found no correlation between the expression of a particular gene and clinical markers such as the number of eosinophils and the amount of IgE. Multivariate analysis of the gene expression data in each sample showed a very high coefficient of correlation among the copy numbers of each gene. The genes under investigation were also expressed in cultured blood eosinophils after IL-4, IL-5 and IFN-gamma stimulation. We were able to predict the function of some of the sequences by scanning for homologies within either the human or mouse genome databases. The mouse counterpart of one of these genes, intersectin 2, was expressed dramatically, as measured by ear edema, in 1-fluoro-2,4-dinitrobenzene-induced mouse contact dermatitis and in NC/Nga mouse dermatitis.
authors.affiliation: Genox Research Inc., Teikyo University Biotechnology Center, 907 Nogawa, Kawasaki, Japan. hashida@genox.co.jp
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Hashida
authors.forename: Kaoru
authors.initials: K
authors.lastname: Ogawa
authors.forename: Masami
authors.initials: M
authors.lastname: Miyagawa
authors.forename: Yuji
authors.initials: Y
authors.lastname: Sugita
authors.forename: Eiki
authors.initials: E
authors.lastname: Takahashi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nagasu
authors.forename: Toshio
authors.initials: T
authors.lastname: Katsunuma
authors.forename: Akira
authors.initials: A
authors.lastname: Akasawa
authors.forename: Gozoh
authors.initials: G
authors.lastname: Tsujimoto
authors.forename: Kenji
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Hirohisa
authors.initials: H
authors.lastname: Saito
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D030541:Databases, Genetic; D003876:Dermatitis, Atopic / Q000097:blood* / Q000235:genetics*; D004804:Eosinophils / Q000502:physiology*; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics*; D005796:Genes / Q000235:genetics*; D015894:Genome, Human; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012720:Severity of Illness Index
chemical_list: D012333:RNA, Messenger