title: Reduced hybrid cluster proteins (HCP) from Desulfovibrio desulfuricans ATCC 27774 and Desulfovibrio vulgaris (Hildenborough): X-ray structures at high resolution using synchrotron radiation.
abstract: The hybrid cluster proteins from the sulfate reducing bacteria Desulfovibrio desulfuricans ATCC 27774 ( Dd) and Desulfovibrio vulgaris strain Hildenborough ( Dv) have been isolated and crystallized anaerobically. In each case, the protein has been reduced with dithionite and the crystal structure of the reduced form elucidated using X-ray synchrotron radiation techniques at 1.25 A and 1.55 A resolution for Dd and Dv, respectively. Although the overall structures of the proteins are unchanged upon reduction, there are significant changes at the hybrid cluster centres. These include significant movements in the position of the iron atom linked to the persulfide moiety in the oxidized as-isolated proteins and the sulfur atom of the persulfide itself. The nature of these changes is described and the implications with respect to the function of hybrid cluster proteins are discussed.
authors.affiliation: Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. República, Apartado 127, 2781-901 , Oeiras, Portugal.|European Synchrotron Radiation Facility, BP 220, 38043 Cedex, Grenoble , France.
authors.forename: David
authors.initials: D
authors.lastname: Aragão
authors.affiliation: Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. República, Apartado 127, 2781-901 , Oeiras, Portugal.|European Synchrotron Radiation Facility, BP 220, 38043 Cedex, Grenoble , France.
authors.forename: Sofia
authors.initials: S
authors.lastname: Macedo
authors.affiliation: European Synchrotron Radiation Facility, BP 220, 38043 Cedex, Grenoble , France.
authors.forename: Edward P
authors.initials: EP
authors.lastname: Mitchell
authors.affiliation: Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. República, Apartado 127, 2781-901 , Oeiras, Portugal.
authors.forename: Célia V
authors.initials: CV
authors.lastname: Romão
authors.affiliation: Department of Biochemistry, University of Georgia, Athens, GA , 30602, USA.
authors.forename: Ming Y
authors.initials: MY
authors.lastname: Liu
authors.affiliation: Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. República, Apartado 127, 2781-901 , Oeiras, Portugal.
authors.forename: Carlos
authors.initials: C
authors.lastname: Frazão
authors.affiliation: Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. República, Apartado 127, 2781-901 , Oeiras, Portugal.
authors.forename: Lígia M
authors.initials: LM
authors.lastname: Saraiva
authors.affiliation: Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. República, Apartado 127, 2781-901 , Oeiras, Portugal.
authors.forename: António V
authors.initials: AV
authors.lastname: Xavier
authors.affiliation: Department of Biochemistry, University of Georgia, Athens, GA , 30602, USA.
authors.forename: Jean
authors.initials: J
authors.lastname: LeGall
authors.affiliation: Department of Biochemistry, Wageningen University, Dreijenlaan 3, 6703 HA , Wageningen, The Netherlands.
authors.forename: Walter M A M
authors.initials: WMAM
authors.lastname: van Dongen
authors.affiliation: Kluyver Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC , Delft, The Netherlands.
authors.forename: Wilfred R
authors.initials: WR
authors.lastname: Hagen
authors.affiliation: Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. República, Apartado 127, 2781-901 , Oeiras, Portugal.
authors.forename: Miguel
authors.initials: M
authors.lastname: Teixeira
authors.affiliation: Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. República, Apartado 127, 2781-901 , Oeiras, Portugal.
authors.forename: Maria A
authors.initials: MA
authors.lastname: Carrondo
authors.affiliation: Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. República, Apartado 127, 2781-901 , Oeiras, Portugal. lindley@itqb.unl.pt.|European Synchrotron Radiation Facility, BP 220, 38043 Cedex, Grenoble , France. lindley@itqb.unl.pt.
authors.forename: Peter
authors.initials: P
authors.lastname: Lindley
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D003901:Desulfovibrio / Q000737:chemistry* / Q000528:radiation effects; D016969:Desulfovibrio vulgaris / Q000737:chemistry* / Q000528:radiation effects; D015151:Immunoblotting; D008958:Models, Molecular; D008968:Molecular Conformation; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000737:chemistry; D011506:Proteins / Q000737:chemistry* / Q000528:radiation effects; D017356:Synchrotrons
chemical_list: D011506:Proteins; D010088:Oxidoreductases; C021402:hydroxylamine reductase