Chemical RNA labeling without 3' end bias using fluorescent cis-platin compounds.
Recently, fluorescent, monofunctional cis-platin derivatives have been developed to chemically label nucleic acids for use in fluorescent hybridization assays. Here we show by hybridizations to microarrays containing oligonucleotide probes for the 3' ends, middle parts, and 5' ends of mRNAs, that this labeling methodology bypasses the problem of the 3' end bias that is characteristic of the conventional enzymatic oligo(dT)-primed, reverse transcription labeling of mRNAs.
Hagedoorn
R
R
Leiden University Medical Center, Leiden, The Netherlands.
Joseph
R
R
Kasanmoentalib
S
S
Eilers
P
P
Killian
J
J
Raap
A K
AK
D004247:DNA / Q000737:chemistry* / Q000235:genetics; D005456:Fluorescent Dyes; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D019169:Jurkat Cells; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012313:RNA / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods*
D005456:Fluorescent Dyes; D012313:RNA; D004247:DNA