Co-immobilized microbial biosensor for BOD estimation based on sol-gel derived composite material.
A novel type of biochemical oxygen demand (BOD) biosensor was developed for water monitor, based on co-immobilizing of Trichosporon cutaneum and Bacillus subtilis in the sol-gel derived composite material which is composed of silica and the grafting copolymer of poly (vinyl alcohol) and 4-vinylpyridine (PVA-g-P(4-VP)). Factors that influence the performance of the resulting biosensor were examined. The biodegradable substrate spectrum could be expanded by the co-immobilized microorganisms. The biosensor prepared also exhibited good reproducibility and long-term stability. Good agreement was obtained between the results of the sensor BOD measurement and those obtained from conventional BOD(5) method for water samples.
Jia
Jianbo
J
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 130022 Changchun, China.
Tang
Mingyu
M
Chen
Xu
X
Qi
Li
L
Dong
Shaojun
S
D001412:Bacillus subtilis / Q000502:physiology*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D018914:Cells, Immobilized; D018920:Coculture Techniques; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste / Q000032:analysis; D008420:Manufactured Materials; D010100:Oxygen / Q000032:analysis* / Q000737:chemistry; D010101:Oxygen Consumption / Q000502:physiology*; D044367:Phase Transition; D011142:Polyvinyl Alcohol; D011725:Pyridines; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide; D014250:Trichosporon / Q000502:physiology*; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000378:metabolism
D007220:Industrial Waste; D011725:Pyridines; D014874:Water Pollutants, Chemical; C414959:poly(vinyl alcohol) grafting 4-vinylpyridine; D012822:Silicon Dioxide; D011142:Polyvinyl Alcohol; D010100:Oxygen