[The study of human papilloma viruses infection in juvenile pharynlaryngeal papillomatosis by PCR and dot blot hybridization].
OBJECTIVE
To study the relationship between infection of human papillomavirus and juvenile pharynlaryngeal papilloma.


METHODS
Polymerase chain reaction (PCR) and dot blot hybridization were used to detect HPV6,11,16,18,33 DNA of 35 samples of pharynlaryngeal papilloma and 10 samples of vocal nodule.


RESULTS
The positive rate of HPV in pharynlaryngeal papilloma was 91.4% (30/35). Among them the positive rates of HPV6 and HPV11 were 54.2%(19/35) and 25.7%(9/35), the positive rate of multiple types of HPV6 + 11 was 11.4%(4/35). The positive rate of HPV16,18,33 was negative. The positive rate of HPV in vocal nodule was negative and were significantly different from that of the pharynlaryngeal papilloma group.


CONCLUSIONS
The results suggest that infection with HPV, especially with HPV6, is closely associated with the development of juvenile pharynlaryngeal papilloma in Wenzhou area. HPV-DNA detected by PCR and dot blot hybridization has highly specificity and sensitivity.
Chen
B
B
Department of Otorhinolaryngology, Second Affiliated Hospital of Wenzhou College of Medical Science, Wenzhou, Zhejiang 325027, China. yxx@dmrc.com.cn
Bao
Q
Q
Zhang
H
H
D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D007223:Infant; D007822:Laryngeal Neoplasms / Q000821:virology*; D008297:Male; D009693:Nucleic Acid Hybridization; D010212:Papilloma / Q000821:virology*; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000821:virology*; D010610:Pharyngeal Neoplasms / Q000821:virology*; D016133:Polymerase Chain Reaction
D004279:DNA, Viral