Identification of self-epitopes recognized by T cells in rheumatoid arthritis demonstrates matrix metalloproteinases as a novel T cell target.
OBJECTIVE
To identify novel arthritis-associated and/or cartilage-specific self-epitopes recognized by T cells in patients with rheumatoid arthritis (RA).


METHODS
Human analogs of several self-epitopes recognized in the rat adjuvant arthritis (AA) model (n = 13) were tested for T cell recognition in patients with RA and healthy controls. Recognition was assessed by proliferative activity of peripheral blood mononuclear cells (PBMC). In addition, cytokine production was determined.


RESULTS
Six out of the 13 peptides recognized during AA were also recognized by more than 20% of the RA patients, in contrast to only one out of the 16 control peptides that were not recognized during AA. The highest proliferative responses were to matrix metalloproteinase (MMP)-derived peptides. The response to a MMP-1 epitope was significantly higher in RA patients than in healthy controls. Moreover, this MMP-1 epitope increased interleukin 4 (IL-4) production of RA PBMC and decreased IL-4 production by control PBMC. The proliferative response to a MMP-3 epitope was similar in RA patients and controls; however, the MMP-3 epitope increased IL-4, and concomitantly IL-1beta and tumor necrosis factor-a production of RA PBMC, whereas these cytokines were unaffected in control PBMC.


CONCLUSIONS
This study shows the presence of immune reactions to MMP-derived T cell epitopes that are associated with RA, suggesting a novel role of MMP in RA.
ter Steege
Jessica
J
Department of Rheumatology and Clinical Immunology, University Medical Center Utrecht, The Netherlands.
Vianen
Marieke
M
van Bilsen
Jolanda
J
Bijlsma
Johannes
J
Lafeber
Floris
F
Wauben
Marca
M
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001172:Arthritis, Rheumatoid / Q000276:immunology* / Q000378:metabolism; D001324:Autoantigens / Q000276:immunology; D002455:Cell Division / Q000276:immunology; D000939:Epitopes; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D005260:Female; D006801:Humans; D015847:Interleukin-4 / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000276:immunology; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000276:immunology*; D019278:Matrix Metalloproteinase 3 / Q000276:immunology*; D008875:Middle Aged; D010446:Peptide Fragments / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
D001324:Autoantigens; D000939:Epitopes; D018984:Epitopes, T-Lymphocyte; D010446:Peptide Fragments; D015847:Interleukin-4; D019278:Matrix Metalloproteinase 3; D020781:Matrix Metalloproteinase 1