Growth phase-dependent changes in the subcellular localization of pre-B-cell colony-enhancing factor.
A cDNA encoding the homolog of the human pre-B-cell colony-enhancing factor (PBEF), a cytokine-like secreted protein, was isolated from a rat cDNA library. This protein existed in both the cytoplasm and nucleus of the cells, and the amount was higher in the cytoplasm than in the nucleus of proliferating PC-12 and Swiss 3T3 cells but higher in the nucleus than in the cytoplasm of the PC-12 cells treated with nerve growth factor and the 3T3 cells grown to a confluent state. Thus, the so-called PBEF is not a cytokine-like secreted protein but an intracellular protein associated with the cell cycle.
Kitani
Takako
T
Department of Biochemistry, Asahikawa Medical College, Japan.
Okuno
Sachiko
S
Fujisawa
Hitoshi
H
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002453:Cell Cycle; D003001:Cloning, Molecular; D016207:Cytokines / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D015723:Gene Library; D007150:Immunohistochemistry; D051379:Mice; D008969:Molecular Sequence Data; D054409:Nicotinamide Phosphoribosyltransferase; D016716:PC12 Cells; D051381:Rats; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014162:Transfection
D016207:Cytokines; D018076:DNA, Complementary; D054409:Nicotinamide Phosphoribosyltransferase; C085455:nicotinamide phosphoribosyltransferase, human