Favourable influence of low molecular weight heparin in mitigating the peroxidative membrane damage induced by a cytotoxic agent and an atherogenic diet.
The present study is aimed to demonstrate the protective effect of a heparin derivative, low molecular weight heparin (LMWH) against erythrocyte membrane injury. Two models serve to induce membrane lipid peroxidative damage, namely a potent cytotoxic agent, adriamycin and a hypercholesterolemic atherogenic diet. Two groups of male Wistar rats (140 +/- 10 g) received a single intravenous injection of adriamycin (ADR, 7.5 mg/kg), while two other groups were fed an atherogenic diet comprising a supplementation of 4% cholesterol, 1% cholic acid and 0.5% thiouracil (CCT diet) for 2 weeks. For each of the above two groups, LMWH (Troparin; 300 microg/day per rat subcutaneously) treatment commenced on day 8 and continued for a week. One group was maintained as the normal control group, and another group that received only LMWH treatment was designated as the LMWH drug control group. Erythrocyte membrane was isolated and assayed for its cholesterol levels, lipid peroxidation and ATPases activity. The activities of antioxidant enzymes were assessed in the haemolysate. The findings of the study were that both adriamycin and the atherogenic diet produced elevated membrane cholesterol levels and lipid peroxidation. The membrane ATPases suffered loss in activity. Accentuated oxidative stress was marked by rise in the activities of antioxidant enzymes (SOD, catalase and GPx). LMWH intervention reverted these changes thereby normalizing the membrane composition and function. The membrane protective effect of LMWH is illuminated by this work.
Deepa
P R
PR
Department of Medical Biochemistry, Dr ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai, India.
Varalakshmi
P
P
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000633:toxicity*; D004036:Diet, Atherogenic; D004195:Disease Models, Animal; D004317:Doxorubicin / Q000008:administration & dosage / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D004910:Erythrocyte Membrane / Q000737:chemistry* / Q000187:drug effects* / Q000378:metabolism; D006495:Heparin, Low-Molecular-Weight / Q000494:pharmacology*; D007275:Injections, Intravenous; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D010544:Peroxidases / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D000903:Antibiotics, Antineoplastic; D006495:Heparin, Low-Molecular-Weight; D004317:Doxorubicin; D010544:Peroxidases