title: Specific sensitization to the common housefly (Musca domestica) not related to insect panallergy.
abstract: BACKGROUND
  Allergy to houseflies is rare. We report a case of respiratory allergy from occupational exposure to houseflies in a farmer.
  
  
  METHODS
  A 30 year-old female farmer with a long-standing history of grass pollen allergy observed for 2 years rhino-conjunctivitis and mild asthma when entering livestock stables and barns. Allergy retesting revealed sensitization to various pollens but not to animal danders. Houseflies (Musca domestica) occurring on the farm in great quantity were suspected by the farmer herself as the causative agent.
  
  
  RESULTS
  Skin prick testing with housefly was positive in the patient and negative in four controls. Experimental radioallergosorbant test was class 3 positive. Sensitization to house dust mite, storage mites and cockroach was not detectable. Western blots with housefly extracts revealed immunoglobulin E (IgE)-binding to bands of 70, 50, and approximately 16 kDa. Tropomyosin in the housefly extract (35 kDa) was recognized by a tropomyosin reference serum but not by the patient. In enzyme-linked immunosorbent assay (ELISA) inhibition assays using housefly as the solid phase, IgE-binding of the patient was inhibited by 75% by M. domestica and by 44% by the closely related lesser housefly (Fannia canicularis), but not by extracts from blowfly (Lucilia spp.), fruit fly (Drosophila spp.), horsefly (Haematopota pluvialis) and mosquito (Culex pipiens). The IgE-binding of the tropomyosin control serum was inhibited by 60-80% by all species.
  
  
  CONCLUSIONS
  In accordance with previous reports, this case demonstrates that respiratory sensitization to insects may be highly specific. According to ELISA inhibition, cross-sensitization in the present case was restricted to species of the family of true flies (Muscidae).
authors.affiliation: Floridsdorf Allergy Centre, Vienna, Austria.
authors.forename: M
authors.initials: M
authors.lastname: Focke
authors.forename: W
authors.initials: W
authors.lastname: Hemmer
authors.forename: S
authors.initials: S
authors.lastname: Wöhrl
authors.forename: M
authors.initials: M
authors.lastname: Götz
authors.forename: R
authors.initials: R
authors.lastname: Jarisch
authors.forename: H
authors.initials: H
authors.lastname: Kofler
mesh_terms: D000328:Adult; D000818:Animals; D005260:Female; D006793:Houseflies; D006801:Humans; D009784:Occupational Diseases / Q000209:etiology*; D016273:Occupational Exposure / Q000009:adverse effects*; D012130:Respiratory Hypersensitivity / Q000209:etiology*