title: Vascular changes play a role in the pathogenesis of necrotizing enterocolitis in asphyxiated newborn pigs.
abstract: Necrotizing enterocolitis (NEC) is the most common acquired gastrointestinal emergency in neonates. We have developed an animal model of NEC in asphyxiated newborn pigs and investigated the effects of asphyxia on blood flow in superior mesenteric artery and abdominal aorta, cardiovascular data, arterial acid-base and blood gas parameters, and endothelial cytoskeletal structure in mesenteric microvasculature. Anesthetized, mechanically ventilated newborn pigs were included in two groups: piglets underwent severe asphyxia, and sham-operated control animals. A cardiovascular and metabolic failure developed in asphyxiated piglets approximately 1 h after the induction: severe hypotension and bradyarrhythmia were seen and significant reductions of the blood flow were measured in the superior mesenteric artery and abdominal aorta during the critical phase. Rearrangement of cytoskeletal actin structure corresponding to enhanced vascular permeability was seen with bodipy phallacidin in mesenterial endothelium of asphyxiated piglets after a 24-h recovery period. In conclusion, severe vasomotor changes during asphyxia may result in mesenteric endothelial dysfunction implicated in increased vascular permeability, edema formation, and development of NEC in asphyxiated piglets.
authors.affiliation: Department of Paediatrics, University of Szeged, Korányi fasor 14-15, 6720 Szeged, Hungary.
authors.forename: B
authors.initials: B
authors.lastname: Gellén
authors.forename: J
authors.initials: J
authors.lastname: Kovács
authors.forename: L
authors.initials: L
authors.lastname: Németh
authors.forename: P
authors.initials: P
authors.lastname: Németh
authors.forename: J
authors.initials: J
authors.lastname: Vágvölgyi
authors.forename: F
authors.initials: F
authors.lastname: Bari
authors.forename: P
authors.initials: P
authors.lastname: Megyeri
authors.forename: S
authors.initials: S
authors.lastname: Pintér
authors.forename: P
authors.initials: P
authors.lastname: Temesvári
authors.forename: M A
authors.initials: MA
authors.lastname: Deli
authors.forename: M
authors.initials: M
authors.lastname: Vecsernyés
authors.forename: Z
authors.initials: Z
authors.lastname: Szilvássy
authors.forename: M
authors.initials: M
authors.lastname: Koltai
authors.forename: C S
authors.initials: CS
authors.lastname: Abrahám
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001237:Asphyxia / Q000150:complications*; D002318:Cardiovascular Diseases / Q000209:etiology / Q000503:physiopathology; D020345:Enterocolitis, Necrotizing / Q000209:etiology / Q000503:physiopathology*; D005260:Female; D007422:Intestines / Q000098:blood supply* / Q000473:pathology; D007511:Ischemia; D008297:Male; D023421:Models, Animal; D013152:Splanchnic Circulation / Q000502:physiology*; D013552:Swine