Sensitivity of PCR detection of Cyclospora cayetanensis in raspberries, basil, and mesclun lettuce.
The sensitivity of a polymerase chain reaction (PCR) method for detection of Cyclospora cayetanensis in raspberries, basil, and mesclun lettuce was evaluated. The assay could detect 40 or fewer oocysts per 100 g of raspberries or basil, but had a detection limit of around 1000 per 100 g in mesclun lettuce.
Steele
Marina
M
Laboratory Services Division, University of Guelph, 95 Stone Road West, Ontario, N1H 8J7, Guelph, Canada.
Unger
Scott
S
Odumeru
Joseph
J
D021744:Cyclospora / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology; D005516:Food Microbiology; D005638:Fruit / Q000469:parasitology*; D006801:Humans; D018545:Lactuca / Q000469:parasitology*; D018649:Ocimum basilicum / Q000469:parasitology*; D033661:Oocysts / Q000302:isolation & purification; D016133:Polymerase Chain Reaction