A validated method development for ketoprofen by a flow-injection analysis with UV-detection and its application to pharmaceutical formulations.
A simple, sensitive and fast flow-injection analysis method with UV-detection method was developed for the determination of ketoprofen in pharmaceuticals. The standard and sample solutions were dissolved in a 10% ethanol which was suitable for this study. A flow-rate of 0.6 ml min(-1) was used and the analyte was monitored at 260 nm. Variables such as concentrations, flow rate of reagents and other flow injection parameters were optimized to produce the most sensitive and reproducible results. Linear calibration curves were obtained in the range of 1.6 x 10(-6) and 1.7 x 10(-4) M. Limit of detection and limit of quantification were 1.7 x 10(-6) M (S/N=3.3) and 5.3 x 10(-6) M (S/N=10), respectively. The method was applied successfully to the analysis of ketoprofen pharmaceutical tablets. The recoveries were 102.75% for peak area and 98.42% for peak height. The proposed method is fast, precise, sensitive and easy to use for the determination of ketoprofen in pharmaceuticals.
Aboul-Enein
Hassan Y
HY
Biological and Medical Research Department (MBC 03-65), Pharmaceutical Analysis Laboratory, King Faisal Specialist Hospital and Research Centre, PO Box 3354, Riyadh 11211, Saudi Arabia. enein@kfshrc.edu.sa
Dal
Arin Gul
AG
Tuncel
Muzaffer
M
D002626:Chemistry, Pharmaceutical; D017022:Flow Injection Analysis / Q000379:methods; D007660:Ketoprofen / Q000032:analysis* / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013678:Technology, Pharmaceutical / Q000379:methods*
D007660:Ketoprofen