[A specific staining for the middle piece of sperm and its possible application].
A special staining technique for the middle piece of sperms is described in different species (cow, horse, sheep, goat, pig). Staining was performed with frozen or fresh semen. The mitochondria in the middle piece could not always be stained with the same intensity. Using 600-fold magnification, subunits of the middle-piece could be observed, but only on fresh collected and immediately stained sperms. When kept in the refrigerator for more than 3 days, the observed subunits disappeared. A positive correlation between staining intensity and enzyme activity could be indicative for sperm motility and also fertilizing capacity.
Weilenmann
R
R
F. Hoffmann-La Roche AG, Basel. richard.weilenmann@roche.com
D000818:Animals; D002417:Cattle; D006041:Goats; D006736:Horses; D008297:Male; D008928:Mitochondria / Q000473:pathology; D012661:Semen / Q000166:cytology; D012756:Sheep; D013045:Species Specificity; D013081:Sperm Motility; D013094:Spermatozoa / Q000002:abnormalities / Q000502:physiology*; D013194:Staining and Labeling / Q000662:veterinary*; D013552:Swine