Rapid biosynthesis of N-linolenoyl-L-glutamine, an elicitor of plant volatiles, by membrane-associated enzyme(s) in Manduca sexta.
In response to elicitors in the oral secretions of caterpillars, plants produce and release volatile chemicals that attract predators and parasitoids of the caterpillar while it feeds. The most prevalent elicitors are fatty acid amides consisting of 18-carbon polyunsaturated fatty acids coupled with l-glutamine. We demonstrate rapid CoA- and ATP-independent in vitro biosynthesis of the fatty acid amide elicitor, N-linolenoyl-l-glutamine, by microsomal fractions of several alimentary tissues in Manduca sexta. N-linolenoyl-l-glutamine is a structural analog of several other elicitors including volicitin, the first fatty acid amide elicitor identified in caterpillars. The enzyme(s) that catalyzed biosynthesis of N-linolenoyl-l-glutamine was localized within the integral membrane protein fraction extracted from microsomes by Triton X-114 detergent phase partitioning and had maximum activity at alkaline pH. We found no evidence suggesting microbial or tissue-independent biosynthesis of N-linolenoyl-l-glutamine in M. sexta. The in vitro biosynthesis of N-linolenoyl-l-glutamine by membrane-associated enzyme(s) in M. sexta represents direct evidence of fatty acid amide synthesis by caterpillar tissues.
Lait
Cameron G
CG
United States Department of Agriculture-Agricultural Research Service, Center for Medical, Agricultural, and Veterinary Entomology, 1700 SW 23rd Drive, Gainesville, FL 32608, USA.
Alborn
Hans T
HT
Teal
Peter E A
PE
Tumlinson
James H
JH
D000818:Animals; D003902:Detergents; D004064:Digestive System / Q000378:metabolism; D005973:Glutamine / Q000031:analogs & derivatives* / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007700:Kinetics; D008042:Linolenic Acids / Q000096:biosynthesis*; D018402:Manduca / Q000201:enzymology / Q000378:metabolism*; D008861:Microsomes / Q000378:metabolism; D017830:Octoxynol; D010944:Plants / Q000378:metabolism / Q000469:parasitology; D011092:Polyethylene Glycols; D012469:Salivary Glands / Q000378:metabolism; D013696:Temperature; D014835:Volatilization
D003902:Detergents; D008042:Linolenic Acids; C475661:N-linolenoyl-L-glutamine; D005973:Glutamine; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40