title: Antibacterial activity of synthetic human B defensin-2 against periodontal bacteria.
abstract: The oral epithelium is continuously exposed to a variety of microbial challenges that can cause infectious diseases such as periodontal disease. Human B Defensin-2 (hBD-2) is a cationic antimicrobial peptide with low molecular weight, which is inducible from oral epithelial cells upon either bacterial infection or stimulation with inflammatory cytokines. This peptide has a broad antimicrobial spectrum that includes gram-positive bacteria, gram-negative bacteria, and fungi. Therefore, it is thought that hBD-2 plays an important role as one of natural immunities to bacterial infection. However, its activity is inhibited by body fluids such as serum. The aim of this study was to assess the antibacterial activity of synthetic hBD-2 against oral bacteria in the presence of saliva or serum. The antibacterial activity of synthetic hBD-2 was tested against Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Streptococcus mutans, and Escherichia coli. Antibacterial broth assay and diffusion assay were performed in vitro. The antibacterial activity of hBD-2 was approximately equal to that of minocycline at equimolar concentrations. Furthermore, the activity of hBD-2 remained at 60% in the presence of 80% saliva, whereas no activity remained in the presence of 20% serum. Our results suggest the possibility that synthetic hBD-2 could be useful to prevent infection by periodontal bacteria.
authors.affiliation: Okayama University Graduate School of Medicine and Dentistry, Japan.
authors.forename: Fumi
authors.initials: F
authors.lastname: Mineshiba
authors.forename: Shogo
authors.initials: S
authors.lastname: Takashiba
authors.forename: Junji
authors.initials: J
authors.lastname: Mineshiba
authors.forename: Kaori
authors.initials: K
authors.lastname: Matsuura
authors.forename: Susumu
authors.initials: S
authors.lastname: Kokeguchi
authors.forename: Yoji
authors.initials: Y
authors.lastname: Murayama
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans / Q000187:drug effects; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D001431:Bacteriological Techniques; D001790:Blood Physiological Phenomena; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D010510:Periodontal Diseases / Q000382:microbiology*; D016966:Porphyromonas gingivalis / Q000187:drug effects; D012463:Saliva / Q000502:physiology; D013295:Streptococcus mutans / Q000187:drug effects; D023083:beta-Defensins / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; C106641:DEFB4A protein, human; D023083:beta-Defensins