title: Novel protein vaccine candidates against Group B streptococcal infection identified using alkaline phosphatase fusions.
abstract: Using an alkaline phosphatase-based genetic screening method, we identified a number of proteins that are potentially located on the outer surface of Group B streptococcus (Streptococcus agalactiae). In an enzyme-linked immunosorbent assay, antisera raised against two of the proteins, the streptococcal yutD homologue and a subunit of an ABC transporter, recognised clinically important serotypes of Group B streptococcus. In a neonatal rat model, purified IgG from the sera conferred significant levels of protection against a lethal challenge infection. The proteins identified show potential as protein subunit candidates for vaccines against Group B streptococcal disease in neonates.
authors.affiliation: Microscience Ltd., 545 Eskdale Road, Winnersh Triangle, Berks RG41 5TU, Wokingham, UK
authors.forename: Martin J G
authors.initials: MJ
authors.lastname: Hughes
authors.forename: Rebecca
authors.initials: R
authors.lastname: Wilson
authors.forename: Joanne C
authors.initials: JC
authors.lastname: Moore
authors.forename: Jonathan D
authors.initials: JD
authors.lastname: Lane
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Dobson
authors.forename: Phillip
authors.initials: P
authors.lastname: Muckett
authors.forename: Zabin
authors.initials: Z
authors.lastname: Younes
authors.forename: Philippa
authors.initials: P
authors.lastname: Pribul
authors.forename: Andrew
authors.initials: A
authors.lastname: Topping
authors.forename: Robert G
authors.initials: RG
authors.lastname: Feldman
authors.forename: Joseph D
authors.initials: JD
authors.lastname: Santangelo
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines / Q000235:genetics* / Q000494:pharmacology; D003001:Cloning, Molecular; D004926:Escherichia coli; D007074:Immunoglobulin G; D011817:Rabbits; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D013290:Streptococcal Infections / Q000276:immunology / Q000517:prevention & control*; D013292:Streptococcus agalactiae
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D007074:Immunoglobulin G; D011993:Recombinant Fusion Proteins; D000469:Alkaline Phosphatase