title: Developmental regulation of expression of Ran/M1 and Ran/M2 isoforms of Ran-GTPase in mouse testis.
abstract: Two isoforms of Ran-GTPase have been described: Ran/M1 and Ran/M2. Ran/M2 is testis specific, whereas the Ran/M1 isoform is also expressed in somatic tissues. Here we show that both mRNAs, differing in 35 of the 648 nucleotides included in the ORFs, are developmentally regulated during spermatogenesis. Real-time RT-PCR experiments demonstrated that the expression of Ran/M1 and Ran/M2 increased in pachytene spermatocytes with progressive transcript accumulation until they reached the round spermatid stage, in the seminiferous epithelium of adults. In the testis, the expression of both isoforms was found to be restricted to germ cells. An expression window from early pachytene spermatocytes to late round spermatids was detected by in situ hybridization.
authors.affiliation: Department of Cell and Developmental Biology, Centro de Investigaciones Biológicas, Madrid, Spain.
authors.forename: Pedro P
authors.initials: PP
authors.lastname: López-Casas
authors.forename: Luis A
authors.initials: LA
authors.lastname: López-Fernández
authors.forename: Mario
authors.initials: M
authors.lastname: Párraga
authors.forename: Dora B
authors.initials: DB
authors.lastname: Krimer
authors.forename: Jesús
authors.initials: J
authors.lastname: del Mazo
mesh_terms: D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D017403:In Situ Hybridization; D007527:Isoenzymes / Q000235:genetics; D008297:Male; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013087:Spermatids / Q000201:enzymology; D013090:Spermatocytes / Q000201:enzymology; D013737:Testis / Q000201:enzymology* / Q000254:growth & development*; D020931:ran GTP-Binding Protein / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D007527:Isoenzymes; D012333:RNA, Messenger; D020931:ran GTP-Binding Protein