title: Crystallization and preliminary X-ray analysis of Escherichia coli p20, a novel thiol peroxidase.
abstract: Escherichia coli p20 is a thioredoxin-dependent thiol peroxidase. This protein represents a novel group of antioxidant enzymes that are widely expressed in various pathogenic bacteria and show distant yet significant sequence homology with peroxiredoxins. E. coli p20, overexpressed in E. coli, was crystallized with PEG 4000 and 2-propanol as precipitants using the hanging-drop vapour-diffusion method. Diffraction data were collected to 2.2 A resolution using synchrotron radiation. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 38.97, b = 58.97, c = 127.59 A. The asymmetric unit contains two p20 molecules, with a corresponding V(M) of 2.06 A(3) Da(-1) and a solvent content of 40.4%.
authors.affiliation: School of Chemistry and Center for Molecular Catalysis, Seoul National University, Seoul 151-742, South Korea.
authors.forename: Jongkeun
authors.initials: J
authors.lastname: Choi
authors.forename: Soonwoong
authors.initials: S
authors.lastname: Choi
authors.forename: Jungwon
authors.initials: J
authors.lastname: Choi
authors.forename: Mee-Kyung
authors.initials: MK
authors.lastname: Cha
authors.forename: Il-Han
authors.initials: IH
authors.lastname: Kim
authors.forename: Whanchul
authors.initials: W
authors.lastname: Shin
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000302:isolation & purification; D033903:Periplasmic Proteins / Q000737:chemistry* / Q000302:isolation & purification; D010544:Peroxidases / Q000737:chemistry* / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D029968:Escherichia coli Proteins; D033903:Periplasmic Proteins; D011994:Recombinant Proteins; D010544:Peroxidases; C097049:Tpx protein, E coli