Simultaneous isocratic HPLC determination of vigabatrin and gabapentin in human plasma by dansyl derivatization.
A rapid and low-cost assay for simultaneous vigabatrin (VGA) and gabapentin (GBP) determination is described that can be performed with simple HPLC instrumentation. The method involves derivatization of the primary amine group of VGA and GBP with dansyl chloride followed by isocratic separation (column: microBondapak C-18, 10 microm, 300 x 3.9 mm; mobile phase: 50 mmol/L NaH(2)PO(4) in 40% acetonitrile) at 50 degrees C and fluorometric detection (excitation and emission wavelength: 318 and 510 nm, respectively) of the fluorescent product, which is stable for at least 7 days. Correlation coefficients of the calibration curves are >0.999 with a lower limit of detection of 0.3 microg/mL. Between- and within-run coefficients of variation are below 4.5%, and assay time is 15 minutes. This method may be used for therapeutic drug monitoring in the case of GBP and to control patient compliance in the case of VGA.
Krivanek
Peter
P
Institut für Pharmakologie, Universität Wien, Vienna, Austria. peter.krivanek@univie.ac.at
Koppatz
Karl
K
Turnheim
Klaus
K
D000085:Acetates / Q000097:blood*; D000588:Amines; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003509:Cyclohexanecarboxylic Acids; D003619:Dansyl Compounds / Q000032:analysis*; D000077206:Gabapentin; D006801:Humans; D020888:Vigabatrin / Q000097:blood*; D005680:gamma-Aminobutyric Acid
D000085:Acetates; D000588:Amines; D003509:Cyclohexanecarboxylic Acids; D003619:Dansyl Compounds; D005680:gamma-Aminobutyric Acid; D000077206:Gabapentin; D020888:Vigabatrin; C029038:dansyl chloride