title: Analysis of two additional loci in Neurospora crassa related to Spore killer-2.
abstract: Two new loci found in one strain of Neurospora crassa (P2604) collected in Malaya are related to the meiotic drive system Spore killer Sk-2. Sk-2 was found in Neurospora intermedia and introgressed into N. crassa. P2604 showed high resistance to killing when crossed to Sk-2. This resistance was found to be linked to, but not allelic to, resistance locus r(Sk-2) on LGIIIL. Analysis showed that the high resistance phenotype of P2604 requires resistance alleles at two different loci on LGIIIR. Strains carrying a resistance allele at only the proximal or the distal locus, respectively, were obtained and intercrossed. Highly resistant strains were obtained by rejoining the two genes. The proximal locus alone confers a low level of resistance. This locus was named pr(Sk-2) for partial resistance to Sk-2. The distal locus was named mod(pr) because its only known phenotype is to modify pr(Sk-2).
authors.affiliation: Department of Biological Sciences, Stanford University, Stanford, CA 949305-5020, USA. barturner@southcoast.net
authors.forename: Barbara C
authors.initials: BC
authors.lastname: Turner
mesh_terms: D000483:Alleles; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D005800:Genes, Fungal; D005819:Genetic Markers; D008296:Malaysia; D008540:Meiosis; D009492:Neurospora crassa / Q000166:cytology / Q000235:genetics*; D013172:Spores, Fungal / Q000235:genetics
chemical_list: D005819:Genetic Markers