title: Genetic diversity of a canine coronavirus detected in pups with diarrhoea in Italy.
abstract: The sequence of the S gene of a field canine coronavirus (CCoV), strain Elmo/02, revealed low nucleotide (61%) and amino acid (54%) identity to reference CCoV strains. The highest correlation (77% nt and 81.7% aa) was found with feline coronavirus type I. A PCR assay for the S gene of strain Elmo/02 detected analogous CCoVs of different geographic origin, all which exhibited at least 92-96% nucleotide identity to each other and to strain Elmo/02. The evident genetic divergence between the reference CCoV strains and the newly identified Elmo/02-like CCoVs strongly suggests that a novel genotype of CCoV is widespread in the dog population.
authors.affiliation: Department of Animal Health and Well-being, Faculty of Veterinary Medicine of Bari, S.p. per Casamassima km 3, 70010, Valenzano, Bari, Italy. a.pratelli@veterinaria.uniba.it <a.pratelli@veterinaria.uniba.it>
authors.forename: Annamaria
authors.initials: A
authors.lastname: Pratelli
authors.forename: Vito
authors.initials: V
authors.lastname: Martella
authors.forename: Nicola
authors.initials: N
authors.lastname: Decaro
authors.forename: Antonella
authors.initials: A
authors.lastname: Tinelli
authors.forename: Michele
authors.initials: M
authors.lastname: Camero
authors.forename: Francesco
authors.initials: F
authors.lastname: Cirone
authors.forename: Gabriella
authors.initials: G
authors.lastname: Elia
authors.forename: Alessandra
authors.initials: A
authors.lastname: Cavalli
authors.forename: Marialaura
authors.initials: M
authors.lastname: Corrente
authors.forename: Grazia
authors.initials: G
authors.lastname: Greco
authors.forename: Domenico
authors.initials: D
authors.lastname: Buonavoglia
authors.forename: Mattia
authors.initials: M
authors.lastname: Gentile
authors.forename: Maria
authors.initials: M
authors.lastname: Tempesta
authors.forename: Canio
authors.initials: C
authors.lastname: Buonavoglia
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002415:Cats; D018352:Coronavirus Infections / Q000662:veterinary* / Q000821:virology; D017939:Coronavirus, Canine / Q000145:classification / Q000235:genetics*; D016765:Coronavirus, Feline / Q000235:genetics; D003967:Diarrhea / Q000662:veterinary* / Q000821:virology; D004283:Dog Diseases / Q000821:virology*; D004285:Dogs; D005243:Feces; D014644:Genetic Variation; D005838:Genotype; D007558:Italy; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics*
chemical_list: D008562:Membrane Glycoproteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins