Photoenhancement of transfection efficiency using novel cationic lipids having a photocleavable spacer.
New cationic lipids having an o-nitrobenzyl moiety as a photocleavable spacer between its hydrophilic and hydrophobic region were synthesized. To improve the efficiency of transfection with lipoplexes, after transfecting the cationic lipid aggregate/DNA complex, photoirradiation was performed. Photochemical decomposition of lipids would not only make the vector's membrane unstable to facilitate the fusion with endocytic vesicles, but also promote dissociation of cationic lipid-DNA complex, thus aiding the escape of DNA from the endocytic vesicles. Using a luciferase gene as a model, we show that UV irradiation of photoresponsive lipoplex-treated COS-1 cells induces a substantial increase in the efficiency of transfection. Herein, we show a novel photoresponsive gene delivery system.
Nagasaki
Takeshi
T
Department of Applied and Bioapplied Chemistry, Graduate School of Engineering, Osaka City University, SORST (JST), Osaka, 558-8585, Japan. nagasaki@bioa.eng.osaka-cu.ac.jp
Taniguchi
Akinobu
A
Tamagaki
Seizo
S
D000818:Animals; D019556:COS Cells; D002412:Cations / Q000138:chemical synthesis / Q000528:radiation effects; D002522:Chlorocebus aethiops; D008055:Lipids / Q000138:chemical synthesis* / Q000528:radiation effects*; D010777:Photochemistry; D014162:Transfection / Q000379:methods*; D014466:Ultraviolet Rays
D002412:Cations; D008055:Lipids