title: Cross-reactivity between the major Parietaria allergen and rotavirus VP4 protein.
abstract: BACKGROUND
  The present study investigates immunological cross-reactivity between Par o 1, the major pollen allergen of Parietaria, and the VP4 protein of rotavirus, a microorganism that is world-wide the main etiological agent of gastroenteritis in children.
  
  
  METHODS
  IgG and IgE cross-reactivity was assessed by direct binding and competitive inhibition assays (ELISA and DARIA), using recombinant VP4 from rhesus infectious rotavirus (RR), synthetic peptides and Par o 1-specific antibodies affinity purified from pooled and individual human sera.
  
  
  RESULTS
  Antibodies specifically binding Par o 1, affinity purified from the sera of 35 individuals with skin test positivity to Parietaria and from 14 pools, were extensively cross-reactive with RRVP4. Cross-reactive binding was specifically inhibited by synthetic peptides derived from the C-terminal sequences of the VP4 proteins from human and rhesus infectious rotavirus.
  
  
  CONCLUSIONS
  This study reports the first evidence of cross-reactivity between an allergen and a viral antigen.
authors.affiliation: Institute of Genetics and Biophysics Adriano Buzzati Traverso, CNR, Naples, Italy.
authors.forename: C
authors.initials: C
authors.lastname: di Somma
authors.forename: L
authors.initials: L
authors.lastname: Fiore
authors.forename: A
authors.initials: A
authors.lastname: Di Lonardo
authors.forename: B
authors.initials: B
authors.lastname: Ridolfi
authors.forename: C
authors.initials: C
authors.lastname: Garzillo
authors.forename: A
authors.initials: A
authors.lastname: Chersi
authors.forename: C
authors.initials: C
authors.lastname: Buono
authors.forename: T
authors.initials: T
authors.lastname: Menna
authors.forename: A
authors.initials: A
authors.lastname: Ruffilli
mesh_terms: D000485:Allergens / Q000276:immunology*; D000906:Antibodies / Q000276:immunology; D036022:Capsid Proteins / Q000276:immunology*; D003429:Cross Reactions / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D010455:Peptides / Q000494:pharmacology; D010940:Plant Proteins / Q000276:immunology*; D011863:Radioimmunoassay / Q000379:methods
chemical_list: D000485:Allergens; D000906:Antibodies; D036022:Capsid Proteins; D007074:Immunoglobulin G; C072038:Par o I protein, Parietaria officinalis; D010455:Peptides; D010940:Plant Proteins; C060339:VP4 protein, Rotavirus; D007073:Immunoglobulin E