title: Oxylipins and ascospore morphology in the ascomycetous yeast genus Dipodascus.
abstract: Immunofluorescence microscopy was used to assess members of the yeast genus Dipodascus for the presence of 3-hydroxy oxylipins. Fluorescence was associated with the aggregating ascospores in all species tested, thus suggesting the association of 3-hydroxy oxylipins with these cells, especially the surrounding slime sheaths. An ultrastructural study of the ascospores revealed sheaths with indentations, probably caused by the close packing of the ascospores to form clusters. In addition, an increase in the neutral and glycolipid fractions as well as a decrease in the phospholipid fraction during ascosporogenesis in D. ambrosiae was found.
authors.affiliation: Lipid Biotechnology Group, Department of Microbiology, Biochemistry and Food Sciences, University of the Free State, PO Box 339, 9301 Bloemfontein, South Africa.
authors.forename: D P
authors.initials: DP
authors.lastname: Smith
authors.forename: J L F
authors.initials: JL
authors.lastname: Kock
authors.forename: P W J
authors.initials: PW
authors.lastname: van Wyk
authors.forename: C H
authors.initials: CH
authors.lastname: Pohl
authors.forename: E
authors.initials: E
authors.lastname: van Heerden
authors.forename: P J
authors.initials: PJ
authors.lastname: Botes
authors.forename: S
authors.initials: S
authors.lastname: Nigam
mesh_terms: D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D006893:Hydroxyeicosatetraenoic Acids / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D004718:Saccharomycetales / Q000502:physiology* / Q000648:ultrastructure; D013172:Spores, Fungal / Q000502:physiology / Q000648:ultrastructure*
chemical_list: D005231:Fatty Acids, Unsaturated; D006893:Hydroxyeicosatetraenoic Acids; C069265:3-hydroxy-5,8,11,14-eicosatetraenoic acid