[Determination of puerarin in xinmaitong oral liquid by HPLC].
OBJECTIVE
To develop a method to determine the content of puerarin in Xinmaitong oral liquid.


METHODS
HPLC method was used. The separation was performed on Suntek kromasil C18 column with methanol-water containing 0.5% acetic acid (25:75) as a mobile phase and the wavelength of UV detector was 250 nm.


RESULTS
The linearity of puerarin was good and average recovery was 97.8%.


CONCLUSIONS
The method is simple, reliable and sensitive. It also shows good resolation. It can be used in quality control of Xinmaitong oral liquid.
Huang
Y F
YF
Third Affiliated Hospital, Sun Yat-sen University of Medical Sciences, Guangzhou 510630, Guangdong, China.
Zhang
Y M
YM
He
A
A
Cao
X H
XH
D000284:Administration, Oral; D002851:Chromatography, High Pressure Liquid; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry*; D007529:Isoflavones / Q000032:analysis*; D030004:Ligusticum / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D029908:Pueraria / Q000737:chemistry*; D011786:Quality Control
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D007529:Isoflavones; C033607:puerarin