Preservation of pancreas tissue during cold storage assessed by X-ray microanalysis.
Clinical transplantation requires cold storage of tissue for several hours. We have examined the elemental content in exocrine and endocrine cells in mouse pancreas after cold storage by X-ray microanalysis, and in parallel carried out morphological studies. Tissue was stored at 4 degrees C for 4-12 h in Normal Krebs-Ringer's (high Na+/K+ ratio), Modified Krebs-Ringer's (low Na+/K+ ratio), Euro-Collins, University of Wisconsin (UW) solution, and seven modified version of UW solution. Incubation in Normal Krebs-Ringer's solution caused significantly increased Na and decreased K concentrations in contrast to incubation in other solutions. The cellular concentration of Na and Cl followed the concentration in the storage solution. Changes in the endocrine cells were similar to, but less pronounced than those in exocrine cells. Calcium was retained best in UW and some variants of UW, and least in Euro-Collins. This may indicate differences in preservation of secretory granules. Also, morphological studies showed that endocrine cells were less affected than exocrine cells. In conclusion, the only factor determining the intracellular concentration of diffusible ions after cold tissue storage is the ionic composition of the extracellular medium. X-ray microanalysis provides an objective method to assess whether the intracellular ionic composition of tissue is maintained during storage.
Kozlova
Inna
I
Department of Medical Cell Biology, University of Uppsala, Box 571, SE-75123, Uppsala, Sweden.
Roomans
Godfried M
GM
D000818:Animals; D015925:Cryopreservation; D005260:Female; D007552:Isotonic Solutions; D008297:Male; D051379:Mice; D010179:Pancreas / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure; D011859:Radiography; D012964:Sodium / Q000378:metabolism
D007552:Isotonic Solutions; C051865:Krebs-Ringer solution; D012964:Sodium