title: Effect of damage type on stimulation of human excision nuclease by SWI/SNF chromatin remodeling factor.
abstract: To investigate the repair of different types of DNA lesions in chromatin, we prepared mononucleosomes containing an acetylaminofluorene-guanine adduct (AAF-G), a (6-4) photoproduct, or a cyclobutane pyrimidine dimer (CPD) and measured the repair of these lesions by reconstituted 6-factor human excision nuclease. We find that incorporation into nucleosomes inhibits the repair of CPD more severely than repair of the AAF-G adduct and the (6-4) photoproduct. Equally important, we find that SWI/SNF stimulates the removal of AAF-G and (6-4) photoproduct but not of CPD from nucleosomal DNA. These results shed new light on the low rate of repair of CPDs in human cells in vivo.
authors.affiliation: Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA.
authors.forename: Ryujiro
authors.initials: R
authors.lastname: Hara
authors.forename: Aziz
authors.initials: A
authors.lastname: Sancar
mesh_terms: D015073:2-Acetylaminofluorene / Q000031:analogs & derivatives* / Q000494:pharmacology; D001483:Base Sequence; D002843:Chromatin / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D004249:DNA Damage; D004260:DNA Repair; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D003850:Deoxyribonuclease I / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism; D019281:Dimerization; D004591:Electrophoresis, Polyacrylamide Gel; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008027:Light; D008969:Molecular Sequence Data; D009707:Nucleosomes / Q000378:metabolism; D011740:Pyrimidine Dimers / Q000737:chemistry; D013997:Time Factors; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D029968:Escherichia coli Proteins; D009707:Nucleosomes; D011740:Pyrimidine Dimers; C445179:SWI-SNF-B chromatin-remodeling complex; D014157:Transcription Factors; C018776:N-(deoxyguanosin-8-yl)acetylaminofluorene; D015073:2-Acetylaminofluorene; D003851:Deoxyribonucleases; D004706:Endodeoxyribonucleases; D003850:Deoxyribonuclease I; C036385:endodeoxyribonuclease uvrABC; D003849:Deoxyguanosine