title: Transgenic rabbit producing human growth hormone in milk.
abstract: The gene construct WAP(6xHisThr):hGH containing the entire human growth hormone gene (hGH) under the rat whey acidic protein (WAP) promoter regulating the expression in mammary glands of mammals was prepared. The 5' end of the gene was modified by the addition of a sequence encoding six histidine residues and a sequence recognized by thrombin. The gene construct was introduced by microinjection into the male pronucleus of a fertilized oocyte. The founder male rabbit was obtained with the transgene mapping to chromosome 7. The presence of the growth hormone was confirmed in samples of milk collected during the lactation of F1 generation females. The growth hormone can be easily purified by affinity chromatography and cleavage by thrombin to an active form.
authors.affiliation: Department of Biochemistry and Biotechnology, August Cieszkowski Agricultural University of Poznań, ul. Wołyńska 35, 60-637 Poznań, Poland.
authors.forename: Daniel
authors.initials: D
authors.lastname: Lipiński
authors.forename: Jolanta
authors.initials: J
authors.lastname: Jura
authors.forename: Robert
authors.initials: R
authors.lastname: Kalak
authors.forename: Andrzej
authors.initials: A
authors.lastname: Pławski
authors.forename: Marta
authors.initials: M
authors.lastname: Kala
authors.forename: Marlena
authors.initials: M
authors.lastname: Szalata
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Jarmuz
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Korcz
authors.forename: Karolina
authors.initials: K
authors.lastname: Słomska
authors.forename: Jacek
authors.initials: J
authors.lastname: Jura
authors.forename: Piotr
authors.initials: P
authors.lastname: Gronek
authors.forename: Zdzisław
authors.initials: Z
authors.lastname: Smorag
authors.forename: Marek
authors.initials: M
authors.lastname: Pieńkowski
authors.forename: Ryszard
authors.initials: R
authors.lastname: Słomski
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002846:Chromatography, Affinity; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D005819:Genetic Markers; D006639:Histidine / Q000737:chemistry; D019382:Human Growth Hormone / Q000378:metabolism*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008892:Milk / Q000378:metabolism*; D008894:Milk Proteins / Q000235:genetics; D009865:Oocytes / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011817:Rabbits; D051381:Rats; D013917:Thrombin / Q000378:metabolism; D019076:Transgenes
chemical_list: D005819:Genetic Markers; D008894:Milk Proteins; C024471:whey acidic proteins; D019382:Human Growth Hormone; D006639:Histidine; D013917:Thrombin