[Expression of simian virus 40 large T-antigen and p53 protein in chinchilla middle ear epithelial cell line and its significance].
OBJECTIVE
To identify the expression of simian virus 40 large T-antigen (SV40TAg) and p53 protein in the cell nuclei of chinchilla middle ear epithelial cell line and explore the significance of the expression.


METHODS
To examine the expression of SV40TAg and p53 protein in cell nuclei of chinchilla middle ear epithelial cell which had been infected and had not been infected with Ad 12-SV40 hybrid virus by immunocytochemistry method.


RESULTS
All of the cell nuclei in 1 of 8 culturing dishes which infected with the Ad12-SV40 virus expressed the SV40TAg and p53 protein and had cultured over 46 passages. They have become a cell line. All of the cells in rest 7 culturing dishes and the control cells did not express the SV40TAg and p53 protein in their nuclei and died after culturing 6-8 passages.


CONCLUSIONS
The SV40TAg and p53 protein play some important role in process of cell immortalization. The expression of SV40TAg and p53 protein in cell nuclei of CMEE-1 cell line has close interrelations with establishing the CMEE-1 cell line.
Jin
S
S
Department of Otorhynolaryngology, Medical College Hospital, Yanbian University, Yanji, Jilin 133000, China.
Jin
Y
Y
Cui
Z
Z
D000818:Animals; D000957:Antigens, Viral, Tumor / Q000096:biosynthesis*; D002460:Cell Line; D002682:Chinchilla; D004432:Ear, Middle / Q000166:cytology*; D004847:Epithelial Cells / Q000378:metabolism* / Q000821:virology*; D013539:Simian virus 40 / Q000276:immunology*; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis*
D000957:Antigens, Viral, Tumor; D016159:Tumor Suppressor Protein p53