Dynamic glycosylation of the transcription factor CREB: a potential role in gene regulation.
We report that CREB (cyclic AMP-responsive element-binding protein), a transcription factor essential for long-term memory, is O-GlcNAc glycosylated in the mammalian brain. Glycosylation occurs at two sites within the Q2 domain and disrupts the interaction between CREB and TAFII130, thereby repressing the transcriptional activity of CREB in vitro. These findings have important implications for the role of O-GlcNAc glycosylation in gene regulation, and they provide a link between O-GlcNAc and information storage processes in the brain.
Lamarre-Vincent
Nathan
N
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Hsieh-Wilson
Linda C
LC
D000117:Acetylglucosamine / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism / Q000502:physiology; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism* / Q000502:physiology; D005786:Gene Expression Regulation / Q000502:physiology*; D006031:Glycosylation; D006367:HeLa Cells; D006801:Humans; D009237:N-Acetyllactosamine Synthase / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D035381:TATA-Binding Protein Associated Factors / Q000378:metabolism / Q000502:physiology; D035362:Transcription Factor TFIID / Q000378:metabolism / Q000502:physiology; D015533:Transcriptional Activation / Q000502:physiology
D017362:Cyclic AMP Response Element-Binding Protein; C101882:TAF4 protein, human; D035381:TATA-Binding Protein Associated Factors; D035362:Transcription Factor TFIID; D009237:N-Acetyllactosamine Synthase; D000117:Acetylglucosamine