[Study of Newcastle disease virus in the treatment of human laryngeal squamous carcinoma].
OBJECTIVE
To explore the biological value of newcastle disease virus in the therapy of human laryngocarcinoma.


METHODS
Nude mice model bearing laryngocarcinoma were established using human laryngeal squamous carcinoma cell line (Hep-2). Large amounts of Newcastle disease virus(NDV) were injected into the tumor. Changes in carcinoma administrated with the NDV were observed under light and electron microscopes. Isolation of NDV was attempted and sera were examined using the method of enzyme-linked immunosorbent assay (ELISA).


RESULTS
The difference between experimental and control groups was statistically significant, including the average weight of the mice: t' = 2.397 (P < 0.05), the average weight of the tumor tissue: t' = 2.852 (P < 0.05) and the average volume of the tumor tissue: t' = 6.058 (P < 0.01). In the experimental group, the NDV was isolated in the tumor tissue and ELISA was positive. The necrosis of the tumor cell and inflammatory cellular infiltration were found under light and electron microscopes.


CONCLUSIONS
These results indicated that NDV was effective in the treatment of laryngocarcinoma and had no damage to normal tissue.
Wang
J
J
Department of Otorhinolaryngology, Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China. WangJiuLi@0451.com
Yu
J
J
Zhao
C
C
Kang
X
X
Li
X
X
Li
S
S
D000818:Animals; D001691:Biological Therapy; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000628:therapy*; D045744:Cell Line, Tumor; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology / Q000628:therapy*; D051379:Mice; D008819:Mice, Nude; D009522:Newcastle disease virus