The age-related accumulation of dolichol in rat liver may correlate with expectation of life.
In order to test the hypothesis that the ageing-related alteration in membrane lipids might reflect the biological age of rodents, the levels of liver dolichol were assayed by the HPLC procedure in male ad-libitum fed (AL) Sprague-Dawley rats aged 2, 6, 12 and 24 months, and in 24-month-old rats on anti-aging food-restrictions (FR) differing in duration and in their effects on longevity. Results showed that the effects on liver dolichol of FR initiated at 2, 6 and 12 months of age, or initiated at 2 and interrupted at 18 months of age were significantly different, and reflected the differences in the effects of FR on expectation of life (the longer the expected residual lifespan the lower the content in liver dolichol). The conclusion is that assay of the quantity of dolichol in the liver tissue may be used as a marker of the biological age of the animal and therefore as an important biomarker of ageing.
Dolfi
Chiara
C
Dipartimento di Patologia Sperimentale, Biotecnologie Mediche, Infettivologia e Epidemiologia, University of Pisa, Pisa, Italy.
Bergamini
Ettore
E
Carresi
Cristiano
C
Cavallini
Gabriella
G
Donati
Alessio
A
Maccheroni
Marco
M
Parentini
Ilaria
I
Marino
Maria
M
Gori
Zina
Z
D000375:Aging / Q000502:physiology*; D000818:Animals; D002784:Cholesterol / Q000378:metabolism; D004032:Diet; D004286:Dolichols / Q000378:metabolism*; D004435:Eating; D008017:Life Expectancy; D008099:Liver / Q000378:metabolism*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D004286:Dolichols; D002784:Cholesterol