title: [Measurement of baicalin contents by HPLC].
abstract: OBJECTIVE
  To compare the contents of baicalin in the raw medical material and the different prepared medical materials of Scutellaria baicalensis.
  
  
  METHODS
  The contents of baicalin were determined by HPLC. Chromatographic conditions included Hypersil C18 column and the mobile phase consisting of a mixture of methanol-0.04% phosphoric acid (46:54). Baicalin was detected at 280 nm. The standard curve was linear in the range of 0.0280-4.6700 micrograms with correlation coefficient 1.0000. The average recovery of baicalin was 101.22% with RSD = 2.54% (n = 5).
  
  
  RESULTS
  The contents of baicalin were 6.80% in raw material, 6.00% and 6.73% in the different prepared medical materials of S. baicalensis respectively.
  
  
  CONCLUSIONS
  The two different kinds of preparing methods can be used to the raw material of S. baicalensis.
authors.affiliation: First Affiliated Hospital of Guiyang College of Traditional Chinese Medicine, Guiyang 550001, Guizhou, China.
authors.forename: Zhu-ying
authors.initials: ZY
authors.lastname: He
authors.forename: Pei-xue
authors.initials: PX
authors.lastname: Cao
authors.forename: Guang-yi
authors.initials: GY
authors.lastname: Liang
authors.forename: Zai-chuan
authors.initials: ZC
authors.lastname: Liu
authors.forename: Xing-guo
authors.initials: XG
authors.lastname: Wu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis*; D006358:Hot Temperature; D010946:Plants, Medicinal / Q000737:chemistry*; D031364:Scutellaria baicalensis / Q000737:chemistry*; D013678:Technology, Pharmaceutical
chemical_list: D005419:Flavonoids; C038044:baicalin