Schistosoma japonicum strains: differentiation by RAPD and SSR-PCR.
Eight geographical isolates of Schistosoma japonicum from Taiwan and mainland China and one isolate of Schistosoma mansoni were studied by RAPD analysis using six arbitrary primers and SSR-PCR analysis using a (CA)8RY primer. The genetic distance was determined by the percentage of unshared bands. The RAPD and SSR-PCR results showed that the genetic distance between S. mansoni and S. japonicum was more than 0.900 and 0.850 respectively; the genetic distance between the eight geographical isolates of S. japonicum was 0.000 to 0.232 and 0.066 to 0.368 respectively. These results demonstrated the usefulness of RAPD and SSR-PCR for showing the differences of inter- and intra-species of Schistosoma. The results also suggest that there is genetic diversity among the different geographical strains of S. japonicum in China.
Anou
Niu
N
Department of Parasitology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China. niuanou@public.wh.hb.cn
Yanwen
Xiong
X
Youren
Feng
F
D000818:Animals; D002681:China / Q000453:epidemiology; D018509:DNA, Helminth / Q000032:analysis* / Q000235:genetics; D019353:Endemic Diseases / Q000706:statistics & numerical data; D005260:Female; D014644:Genetic Variation / Q000235:genetics; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods* / Q000592:standards; D012549:Schistosoma japonicum / Q000145:classification* / Q000235:genetics*; D012554:Schistosomiasis japonica / Q000453:epidemiology / Q000469:parasitology; D013045:Species Specificity; D013624:Taiwan / Q000453:epidemiology
D018509:DNA, Helminth