[In vitro and in vivo functional evaluation of anti-human bladder tumor human-mouse chimeric antibody ch-BDI].
OBJECTIVE
To evaluate the in vitro and in vivo function of anti-human bladder tumor human-mouse chimeric antibody ch-BDI and its future clinical application.


METHODS
With ch-BDI in high-expression cell-line medium, affinity chromatography was used for the purification. Labeled with (99m)Tc through reduction method, its immunoreactive fraction and association constant were measured. The constant was injected into nude mice with xenografted human bladder tumor. The biodistribution of the labeled ch-BDI was studied with radioimmunoimaging.


RESULTS
ch-BDI showed desirable immunoreactive fraction (76%) and association constant (3.56 x 10(9) M(-1)) in vitro and a terrific specific targeting effect in vivo.


CONCLUSIONS
ch-BDI has fairly good function against human bladder tumor both in vitro and in vivo, and is promising in clinical use.
Bai
Yin
Y
Institute of Urology, First Hospital, Peking University, Beijing 100034, China.
Yu
Li-zhang
LZ
Lü
Ying-qiang
YQ
Ai
Jun-kui
JK
Zhang
Chun-li
CL
Ding
Yi
Y
Wang
Yan
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000912:Antibodies, Neoplasm / Q000276:immunology*; D000915:Antibody Affinity; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011993:Recombinant Fusion Proteins / Q000276:immunology*; D001749:Urinary Bladder Neoplasms / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D011993:Recombinant Fusion Proteins