Histomorphometric characteristics and expression of epidermal growth factor and its receptor by epithelial cells of normal gingiva and hereditary gingival fibromatosis.
OBJECTIVE
The objective of this study was to examine the histomorphometric features and evaluate the expression of epidermal growth factor (EGF) and transmembranic receptor (EGFr) and the proliferative potential of epithelial cells from normal and hereditary gingival fibromatosis (HGF) gingival tissues.


BACKGROUND
EGF is a multifunctional cytokine with a variety of biological effects including stimulation of cell proliferation by binding to its specific EGFr.


METHODS
Immunohistochemistry was performed to measure EGF and EGFr expression and the epithelial cell proliferation was determined by measuring proliferating cell nuclear antigen (PCNA).


RESULTS
Histomorphometric evaluation indicated that in HGF the mean height of the epithelial papillae was higher compared to the normal gingiva (NG), whereas mean epithelial area and number of epithelial papillae were quite similar in both groups. The EGF and EGFr positive cells were observed in the basal, spinous and granular cell layers of both normal and HGF tissues, with a gradual reduction from the basal layer. Although the expressions of EGF and EGFr in the control group were significantly higher than those from HGF, in HGF the epithelial papilla tips showed increased number of proliferating cells and elevated expression of EGF and EGFr. There was a correlation between the proliferative potential of epithelial cells and the expression of EGF or EGFr only in the epithelial papilla tips of HGF gingiva.


CONCLUSIONS
Our data suggest that EGF and EGFr in the oral epithelium of HGF gingiva may stimulate epithelial cell proliferation, with the resultant apical migration of the oral epithelium and formation of the slender deep epithelial papillae; however, without hyperplastic alterations.
Araujo
C S A
CS
Department of Oral Pathology, Dental School, University of Campinas, Piracicaba, SãoPaulo, Brazil.
Graner
E
E
Almeida
O P
OP
Sauk
J J
JJ
Coletta
R D
RD
D000328:Adult; D000704:Analysis of Variance; D002452:Cell Count; D002455:Cell Division / Q000502:physiology; D002462:Cell Membrane / Q000648:ultrastructure; D004396:Coloring Agents; D003593:Cytoplasm / Q000648:ultrastructure; D004815:Epidermal Growth Factor / Q000032:analysis*; D004847:Epithelial Cells / Q000473:pathology; D066246:ErbB Receptors / Q000032:analysis*; D005260:Female; D005351:Fibromatosis, Gingival / Q000235:genetics* / Q000473:pathology; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D018709:Statistics, Nonparametric
D004396:Coloring Agents; D018809:Proliferating Cell Nuclear Antigen; D004815:Epidermal Growth Factor; D066246:ErbB Receptors