title: Characteristics of skin wrinkling and dermal changes induced by repeated application of squalene monohydroperoxide to hairless mouse skin.
abstract: We have studied the effect of squalene monohydroperoxides (Sq-OOH), initial products of UV-peroxidated squalene, on the skin of hairless mice. Repeated topical application of 10 mM Sq-OOH to hairless mice for 15 weeks induced definite skin wrinkling. When image analysis was used to compare wrinkle formation induced by ultraviolet B (UVB) irradiation and Sq-OOH treatment, the degree of wrinkling in exposed skin was seen to be similar. However, the characteristics of wrinkles induced by either method differed markedly with regard to direction and distribution. Biochemical analysis revealed a significant decrease in collagen content per unit area and mass in Sq-OOH-treated skin, whereas no changes per unit area and decrease in collagen per unit mass were observed in UVB-irradiated skin. As for glycosaminoglycan (GAG) content per unit area, significant increases were observed in both Sq-OOH-treated skin and UVB-irradiated skin. These changes were not induced by organic hydroperoxides such as TERT-butylhydroperoxide or cumene hydroperoxide treatment. Histological observation revealed epidermal hyperplasia and dermal alterations such as collagen degradation and GAG increases in Sq-OOH-treated skin. Histological changes induced by Sq-OOH were not as pronounced as those induced by UVB irradiation. These results clearly suggest that the wrinkling and changes in dermal collagen content induced by Sq-OOH are qualitatively different to those induced by UVB exposure. This may provide a useful model for the study of skin aging, particularly with regard to collagen content.
authors.affiliation: Yakult Central Institute for Microbiological Research, Tokyo, Japan. katsuyoshi-chiba@yakult.co.jp
authors.forename: K
authors.initials: K
authors.lastname: Chiba
authors.forename: K
authors.initials: K
authors.lastname: Kawakami
authors.forename: T
authors.initials: T
authors.lastname: Sone
authors.forename: M
authors.initials: M
authors.lastname: Onoue
mesh_terms: D000287:Administration, Topical; D000818:Animals; D001555:Benzene Derivatives / Q000494:pharmacology; D003094:Collagen / Q000378:metabolism; D003238:Connective Tissue / Q000187:drug effects / Q000528:radiation effects; D000431:Ethanol / Q000494:pharmacology; D005260:Female; D006025:Glycosaminoglycans / Q000378:metabolism; D051379:Mice; D008812:Mice, Hairless; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress; D014677:Pharmaceutical Vehicles / Q000494:pharmacology; D012867:Skin / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects*; D015595:Skin Aging / Q000187:drug effects / Q000528:radiation effects*; D013185:Squalene / Q000008:administration & dosage / Q000009:adverse effects / Q000031:analogs & derivatives / Q000378:metabolism*; D014466:Ultraviolet Rays / Q000009:adverse effects*; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
chemical_list: C002821:2,3-oxidosqualene; D001555:Benzene Derivatives; D006025:Glycosaminoglycans; D016877:Oxidants; D014677:Pharmaceutical Vehicles; D000431:Ethanol; D013185:Squalene; D003094:Collagen; D020122:tert-Butylhydroperoxide; C007164:cumene hydroperoxide