title: A new monoclonal antibody, mAb 204-11, that influences the binding of platelet GPVI to fibrous collagen.
abstract: The newly identified platelet collagen receptor glycoprotein VI binds to fibrous collagen, inducing platelet activation. Several antibodies against GPVI have been reported, including a patient's auto-antibodies, that activates platelets through their ability to crosslink this glycoprotein. We have developed a monoclonal antibody (mAb) against GPVI using the recombinant extracellular domain of GPVI as an antigen. This antibody, mAb 204-11, induced platelet aggregation and tyrosine phosphorylation of proteins similar to those induced by GPVI-reactive proteins, collagen and convulxin. Its interaction with GPVI was analyzed by measuring the effect of the antibody on GPVI binding to collagen using a dimeric form of recombinant GPVI, GPVI-Fc2. MAb 204-11 inhibited the binding of GPVI-Fc2 to fibrous collagen particles, but enhanced the GPVI binding to immobilized collagen, suggesting that the antibody binds to a region near the collagen binding site of GPVI. MAb 204-11 also inhibited the GPVI binding to convulxin at a low concentration, but not completely. Since mAb 204-11 reacts specifically with GPVI and is applicable for immunoblotting and immunoprecipitation, this antibody would be useful for studies on GPVI.
authors.affiliation: Department of Protein Biochemistry, Institute of Life Science, Kurume University, 2432-3 Aikawa-machi, Kurume, Fukuoka-ken, 839-0861, Japan. mmoroi@mbox.lsi.kurume-u.ac.jp
authors.forename: Masaaki
authors.initials: M
authors.lastname: Moroi
authors.forename: Jun
authors.initials: J
authors.lastname: Mizuguchi
authors.forename: Sachiko
authors.initials: S
authors.lastname: Kawashima
authors.forename: Michiko
authors.initials: M
authors.lastname: Nagamatsu
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Miura
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Nakagaki
authors.forename: Katsuaki
authors.initials: K
authors.lastname: Ito
authors.forename: Stephanie M
authors.initials: SM
authors.lastname: Jung
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D000937:Antigen-Antibody Reactions; D001665:Binding Sites; D003094:Collagen / Q000737:chemistry / Q000378:metabolism*; D000939:Epitopes; D007141:Immunoglobulin Fc Fragments / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D010980:Platelet Membrane Glycoproteins / Q000276:immunology / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D011994:Recombinant Proteins
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D007141:Immunoglobulin Fc Fragments; D010980:Platelet Membrane Glycoproteins; D011994:Recombinant Proteins; C098341:platelet membrane glycoprotein VI; D003094:Collagen