[The protective effects of yi-xin-kang capsule on the structure and function of rat myocardial mitochondria].
OBJECTIVE
To observe the injury induced by Isoproterenol (ISO) on the structure and function of rat myocardial mitochondria and the protective effects of Yi-Xin-Kang Capsule (H303).


METHODS
After injection of ISO, rat myocardial mitochondria were isolated. The activities of phospholipidase A2(PLA2) and Ca(2+)-ATPase, the content of phospholipids (PL) and high-energy phosphates, membrane lipid fluidity (LFU) and respiration function were determined respectively.


RESULTS
ISO induced severe injury to myocardial mitochondria. The activity of PLA2 was significantly activated. The intensity of LFU, the activity of Ca(2+)-ATPase, respiration function of mitochondria and the contents of PL and high-energy phosphates of myocardium tissue were significantly decreased. Oral administration of H303 significantly decreased the activity of PLA2. The contents of PL and LFU, the activity of Ca(2+)-ATPase were significantly increased. The respiration function of mitochondria was protected by accelerating III state respiration rate, improving ADP/O ratio and recovering the depressed respiration control rate.


CONCLUSIONS
H303 possessed protective effect on structural and functional injury of mitochondria induced by ISO.
Han
L
L
Beijing Institute of Radiation Medicine, Beijing 100850, China.
Chen
K J
KJ
D000818:Animals; D027885:Astragalus propinquus / Q000737:chemistry; D002214:Capsules; D002316:Cardiotonic Agents / Q000494:pharmacology*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007545:Isoproterenol / Q000037:antagonists & inhibitors; D030004:Ligusticum / Q000737:chemistry; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects; D008929:Mitochondria, Heart / Q000378:metabolism* / Q000648:ultrastructure; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D027561:Salvia miltiorrhiza / Q000737:chemistry
D002214:Capsules; D002316:Cardiotonic Agents; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D010741:Phospholipases A; D054467:Phospholipases A2; D007545:Isoproterenol