[Determination of shikimic acid in fruit of Illiciaceae plants by HPLC with diode-array detection].
OBJECTIVE
To establish the determination method of Shikimic acid.


METHODS
Using HPLC method as the determination method. The separation was performed in a SiO2-NH2 column with a mobile phase of Acetonitrile-2% H3PO4 water solution (95:5); The sample wavelength was 213 nm, reference wavelength 300 nm.


RESULTS
The average collection was 98.5%, RSD 1.67% (n = 5).


CONCLUSIONS
This method is suitable for the determination of Shkimic acid in herb medicines and preparation containing shikimic acid.
Wang
X Q
XQ
Beijing University of Chinese Medicine, Beijing 100029, China.
Guo
Y J
YJ
Yang
C S
CS
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005638:Fruit / Q000737:chemistry; D029523:Illicium / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D012765:Shikimic Acid / Q000032:analysis*
D012765:Shikimic Acid