Development of a point-of-care assay system for high-sensitivity C-reactive protein in whole blood.
BACKGROUND
C-reactive protein (CRP) is emerging as a potential risk predictor for future cardiovascular diseases (CVD). High sensitivity assays have been developed and applied for clinical purposes.


METHODS
The fluorescence immunochromatographic assay was employed to detect and quantify CRP in whole blood. It consisted of a fluorescence (FL) antibody detector buffer, a test strip housed in a disposable cartridge, and a laser fluorescence scanner. Whole blood sample was mixed with detector, loaded onto a cartridge, incubated for 10 min, and the concentration of CRP was measured in a laser fluorescence scanner. The linearity, limit of detection (LOD), and performance of new assay system was tested and evaluated. The comparability of assay was examined with an automated reference method.


RESULTS
With the new assay system, a reliable correlation of coefficient (r) was obtained between the ratio value (A(T)/A(C)) and a concentration of CRP in samples. The linearity fell in the range of 0-10 mg/l of CRP, and the analytical detection limit was 0.133 mg/l of CRP. The mean recovery of the control was 105.2% in a working range. The precision of the intra- and inter-assay in a range of 0.5-6 mg/l was CVs <6% and <8%, respectively. The new fluorescence immunochromatographic assay system correlated well with a traditional immunoturbidimetric assay for quantification of CRP concentration (r=0.955, N=90).


CONCLUSIONS
The fluorescence immunochromatographic assay is fast, reliable, and a reproducible platform for point-of-care testing (POCT) of high-sensitive (hs)-CRP in whole blood.
Ahn
Jae Soon
JS
Central Research Institute of BodiTech Inc., Chuncheon, 200-160, South Korea.
Choi
Sunga
S
Jang
Sang Ho
SH
Chang
Hyuk Jae
HJ
Kim
Jae Hoon
JH
Nahm
Ki Bong
KB
Oh
Sang Wook
SW
Choi
Eui Yul
EY
D000328:Adult; D000911:Antibodies, Monoclonal; D002097:C-Reactive Protein / Q000032:analysis*; D002138:Calibration; D002318:Cardiovascular Diseases / Q000097:blood*; D002845:Chromatography / Q000379:methods; D005455:Fluorescent Antibody Technique / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D007834:Lasers; D008875:Middle Aged; D019095:Point-of-Care Systems; D012016:Reference Values; D012680:Sensitivity and Specificity; D044967:Serum / Q000737:chemistry
D000911:Antibodies, Monoclonal; D002097:C-Reactive Protein