Induction of MafBx and Murf ubiquitin ligase mRNAs in rat skeletal muscle after LPS injection.
MafBx and Murf are two new rat E3 ubiquitin ligases induced in muscle atrophy. Our goal was to investigate whether lipopolysaccharide (LPS) injection, a model of muscle catabolism, is associated with increased expression of MafBx and Murf. LPS (750 microg/100 g body weight) induces MafBx and Murf mRNA (respectively, 23-fold and 33-fold after 12 h; P<0.001). A transient induction of tumor necrosis factor-alpha mRNA (21-fold; P<0.001 at 3 h) and a decrease of insulin like growth factor-I mRNA (50%; P<0.001 at 6 h), two potential regulators of the ubiquitin-proteasome system were also demonstrated. In summary, MafBx and Murf mRNA are up-regulated in response to LPS and might play a role in the muscle proteolysis observed.
Dehoux
Mischaël J M
MJ
Unité de Diabétologie et Nutrition, Université catholique de Louvain, B-1200 Brussels, Belgium.
van Beneden
Ronald P
RP
Fernández-Celemín
Laura
L
Lause
Pascale L
PL
Thissen
Jean-Paul M
JP
D000818:Animals; D001835:Body Weight; D008025:Ligases / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000378:metabolism*; D009124:Muscle Proteins / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D044843:SKP Cullin F-Box Protein Ligases; D013997:Time Factors; D000070584:Tripartite Motif Proteins; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases; D015854:Up-Regulation
D008070:Lipopolysaccharides; D009124:Muscle Proteins; D012333:RNA, Messenger; D000070584:Tripartite Motif Proteins; D014409:Tumor Necrosis Factor-alpha; D012313:RNA; C440907:FBXO32 protein, human; C486692:Fbxo32 protein, rat; D044843:SKP Cullin F-Box Protein Ligases; C440908:TRIM63 protein, human; C499086:Trim63 protein, rat; D044767:Ubiquitin-Protein Ligases; D008025:Ligases