title: [Determination of gossypol in cotton root bark by HPLC].
abstract: OBJECTIVE
  To establish a method for the determination of gossypol in cotton root bark.
  
  
  METHODS
  Samples were extracted with acetone, and determined on a C18 column with the mobile phase (Acetonitrile-0.2% phosphoric acid, 85:15) and UV-235 nm detector.
  
  
  RESULTS
  The recovery rate was 97.6%, RSD 1.59% (n = 5).
  
  
  CONCLUSIONS
  This method can be used for the determination of gossypol in cotton root bark and the content of gossypol in three different species has been determined.
authors.affiliation: Hubei College of Traditional Chinese Medicine, Hubei, Wuhan 430061. guanghongcui@263.net
authors.forename: Guang-hong
authors.initials: GH
authors.lastname: Cui
authors.forename: Jia-chun
authors.initials: JC
authors.lastname: Chun
authors.forename: Da-yong
authors.initials: DY
authors.lastname: Cai
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003368:Gossypium / Q000737:chemistry*; D006072:Gossypol / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D006072:Gossypol