[Expressions of angiotensin II type 1 receptor mRNA in fibrotic and normal liver tissues].
OBJECTIVE
To investigate the expression profile of angiotensin    type 1 receptor (AT1R) mRNA in fibrotic and normal liver tissues.


METHODS
Semi-quantitative RT-PCR was used to detect the expression of AT1R mRNA in 18 specimens of fibrotic liver tissues adjacent to liver cancers and 12 normal liver tissue specimens. Specific AT1R product and GAPDH product used as internal control were both amplified by RT-PCR, and the ratio of their integrated optical densities calculated to estimate the relative quantity of AT1R mRNA expression.


RESULTS
The relative mRNA expression of AT1R in fibrotic liver tissues was significantly higher than that of normal liver tissues (1.27+/-0.45 vs 0.71+/-0.21, P < 0.01).


CONCLUSIONS
AT1R may play an important role in the development of hepatic fibrosis.
Wang
Wei-wei
WW
Institute for Digestive Diseases of PLA, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. niehaibo36@hotmail.com
Yang
Xi-shan
XS
Wu
Ping-sheng
PS
Wang
Jie
J
Zhang
Hong-bin
HB
Li
Xu
X
Zheng
Wen-ling
WL
D000328:Adult; D005260:Female; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008103:Liver Cirrhosis / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis*; D044139:Receptor, Angiotensin, Type 2 / Q000235:genetics*; D012084:Renin-Angiotensin System / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger; D044139:Receptor, Angiotensin, Type 2