[Preparation of genistein-loaded chitosan microspheres].
OBJECTIVE
To optimize the preparation of genistein chitosan microspheres with central composite design (CCD).


METHODS
The chitosan microspheres were prepared by the O/W/O multiple emulsion method. Second-order polynomial and linear equations were fitted to the data, and the resulting equations were used to produce response surface graphs and the best experiment conditions.


RESULTS
The theoretical drug content was 13%-15%, the concentration of organic phase was 30%-40% and the concentration of oil phase was 68%-72%.


CONCLUSIONS
The best experiment conditions can be obtained by central composite design and response surface methodology. The observed values agree well with model predicted values.
Wu
Wan-ying
WY
College of Pharmacy, Beijing University of Traditional Chinese Medicine, Beijing 100029, China.
Li
Yun-gu
YG
D000970:Antineoplastic Agents / Q000008:administration & dosage*; D002686:Chitin / Q000031:analogs & derivatives*; D048271:Chitosan; D003692:Delayed-Action Preparations; D019833:Genistein / Q000008:administration & dosage*; D008433:Mathematics; D008863:Microspheres
D000970:Antineoplastic Agents; D003692:Delayed-Action Preparations; D002686:Chitin; D048271:Chitosan; D019833:Genistein