title: The Effects of G Protein Modulators on the Labellar Taste Receptor Cells of the Fleshfly (Boettcherisca peregrina).
abstract: A nonhydrolyzable G protein activator (guanosine 5'-O-(3-thiotriphosphate); GTPgammaS) and a G protein inhibitor (guanosine 5'-O-(2-thiodiphosphate); GDPbetaS) were introduced into the labellar taste receptor cells of the fleshfly by treatment of their receptive membranes beneath the tip opening of the chemosensory hair with each reagent in 0.03% deoxycholate solution for 4 min. After treatment with GTPgammaS, the responses of the sugar receptor cell to D-glucose, D-fructose, L-phenylalanine and L-valine and that of the salt receptor cell to cyclic AMP were markedly enhanced, compared with those after treatment with deoxycholate alone. Treatment with GDPbetaS depressed these responses. These results strongly suggest that the responses are mediated by G protein. However, the response of the salt receptor cell to NaCl was not affected by treatment with either GTPgammaS or GDPbetaS, and thus the response to NaCl clearly is not elicited through a G protein-regulated mechanism. Copyright 1997 Elsevier Science Ltd. All rights reserved
authors.affiliation: Biological Institute, Graduate School of Science, Tohoku University, Aoba-ku, Sendai 980-77, Japan
authors.forename: I
authors.lastname: SHIMADA
authors.forename: M
authors.initials: M
authors.lastname: KOGANEZAWA