[Determination of lignan glycosides in Gaultheria leucocarpa var. yunnanensis by RP-HPLC].
OBJECTIVE
To control the quality of Gaultheria leucocarpa var. yunnanensis the contents of lignans in these plants from different provinces were detected.


METHODS
HPLC was adopted using Hypersil-18 column (0.4 cm x 25 cm), methanol: acetonitrile: water (30:5:65) as mobile phase.


RESULTS
The calibration curves of D1(gaultherinA), D2[(-)-5'-methoxyisolariciresinol-2 alpha-O-beta-D-xylopyranoside)], D4[(+)-lyoniresinol-2 alpha-O-beta-D-glucopyranoside)] were linear in the range of 0.10-0.40 microgram, 0.03-0.13 microgram, 0.02-0.09 microgram, respectively. The average recovery of D1 was 98.9% and RSD was 2.6%.


CONCLUSIONS
Comparing the samples from Jinxiou(158.2 mg.100 g)-1 and Kumming(154.8 mg.100 g)-1, that of Guiyang showed the highest content of LD(208.8 mg.100 g)-1. The content of LD in the tender above-ground part was 1.3 times higher than that in the wilt.
Ma
Xiao-jun
XJ
Department of Natural Medicines, School of Pharmaceutical Science, Beijing University, Beijing 100083, China. xjma@public.bta.net.cn
Zhao
Ling
L
Zhao
Yu-juan
YJ
Du
Cheng-fang
CF
Zheng
Jun-hua
JH
Chen
Xin-zi
XZ
D001565:Benzoates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D031282:Gaultheria / Q000737:chemistry*; D006027:Glycosides / Q000032:analysis; D017705:Lignans / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control
D001565:Benzoates; D006027:Glycosides; D017705:Lignans; C477164:gaultherin A