Carbohydrate and alditol analysis by high-performance anion-exchange chromatography coupled with electrochemical detection at a cobalt-modified electrode.
A cobalt oxyhydroxide film dispersed on a carbon electrode surface was characterized and proposed as an amperometric sensor for determination of alditols and carbohydrates in flowing streams. Complex mixtures of carbohydrates were separated by anion-exchange chromatography using a moderately alkaline solution as mobile phase. The cobalt modified electrode (GC-Co) was employed under a constant applied potential of 0.5 V (vs Ag/AgCl). Under these experimental conditions the detection limits (S/N=3) for all analyzed electroactive molecules ranged between 0.3 micromol L(-1) and 1.5 micromol L(-1) and the dynamic linear ranges spanned generally three orders of magnitude above the relevant detection limits. Analytical determinations of carbohydrates and alditols in red and white wines, are reported.
Casella
Innocenzo G
IG
Dipartimento di Chimica, Universita' degli Studi della Basilicata, Via N. Sauro 85, 85100, Potenza, Italy. casella@unibas.it
Contursi
Michela
M
D002241:Carbohydrates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis; D003035:Cobalt; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013402:Sugar Alcohols / Q000032:analysis*; D014159:Transducers; D014920:Wine / Q000032:analysis* / Q000145:classification
D002241:Carbohydrates; D020099:Coated Materials, Biocompatible; D013402:Sugar Alcohols; D003035:Cobalt