title: Marrow stromal cell recovery after radiation-induced aplasia in mice.
abstract: OBJECTIVE
  The identification of fibroblast-like cells of the marrow stroma by means of alkaline phosphatase (ALP) cytochemistry reveals delicate ALP-positive structures interspersed among haematopoietic cells and arranged in a loosely meshed network. These cells are often referred to as 'reticular' cells and the network they form is known as the 'ALP network'. The purpose was to analyse the evolution of this ALP network in relation to haemopoietic regeneration after whole-body irradiation.
  
  
  METHODS
  The total surface occupied by ALP-positive processes revealed by means of ALP cytochemistry was expressed as a ratio of the total marrow area. ALP-positive cells were counted using nuclei as the defining unit. Cell proliferation was analysed by the detection of bromodeoxyuridine (BrdU) incorporation. Fat cells were identified by oil red O staining and alpha-glycerophosphate dehydrogenase (alpha-GPDH) activity.
  
  
  RESULTS
  The ALP network and ALP-positive cell number began to increase 24 h after 4-Gy irradiation to reach a maximum after 72 h, when the bone marrow was almost completely empty of haemopoietic cells. This increase was in advance of haemopoietic recovery and was not due to cell proliferation. A decrease in the ALP network occurred in parallel with an increase in haemopoiesis and was accompanied by a transient increase in fat cells on day 7.
  
  
  CONCLUSIONS
  These data indicate that the recovery of the ALP network, which is partially due to the recruitment of ALP- positive cells, occurs in advance of the haemopoietic recovery and that the equilibrium between fat cells and ALP-positive cells seems to be controlled by haemopoietic cells.
authors.affiliation: Department of Histology and Cytology, CHU-B23, B-4000 Liege, Belgium.
authors.forename: K
authors.initials: K
authors.lastname: Almohamad
authors.forename: A
authors.initials: A
authors.lastname: Thiry
authors.forename: F
authors.initials: F
authors.lastname: Hubin
authors.forename: Z
authors.initials: Z
authors.lastname: Belaid
authors.forename: C
authors.initials: C
authors.lastname: Humblet
authors.forename: J
authors.initials: J
authors.lastname: Boniver
authors.forename: M-P
authors.initials: MP
authors.lastname: Defresne
mesh_terms: D017667:Adipocytes / Q000473:pathology / Q000528:radiation effects; D000469:Alkaline Phosphatase / Q000378:metabolism; D000741:Anemia, Aplastic / Q000201:enzymology / Q000209:etiology / Q000473:pathology*; D000818:Animals; D001854:Bone Marrow Cells / Q000201:enzymology / Q000473:pathology* / Q000528:radiation effects*; D001973:Bromodeoxyuridine / Q000378:metabolism; D002453:Cell Cycle; D051379:Mice; D008810:Mice, Inbred C57BL; D011832:Radiation Injuries / Q000201:enzymology / Q000209:etiology / Q000473:pathology*; D017154:Stromal Cells / Q000201:enzymology / Q000473:pathology / Q000528:radiation effects
chemical_list: D000469:Alkaline Phosphatase; D001973:Bromodeoxyuridine