[mRNA expression of hyaluronan synthase in the endolymphatic sac cells of guinea pigs].
OBJECTIVE
To detect the mRNA expression of hyaluronan synthase in the endolymphatic sac cells of guinea pigs.


METHODS
After consulting gene bank, we analyzed conservative sequence of hyaluronan synthases in different species, detected the mRNA expression of hyaluronan synthase in the endolymphatic sac cells of guinea pigs with oligonucleotide probe by hybridization in situ.


RESULTS
mRNA of hyaluronan synthase was strongly expressed in some epithelial cells of endolymphatic sac, coupled with negative expression in negative control groups.


CONCLUSIONS
It confirms that endolymphatic sac cells can synthesize hyaluronan.
Mou
Z
Z
Department of Otorhinolaryngology Head and Neck Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China. zhonglm@cta.cq.cn
Liu
Z
Z
Chen
J
J
Wan
Y
Y
D000818:Animals; D004712:Endolymphatic Sac / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D005260:Female; D014453:Glucuronosyltransferase / Q000096:biosynthesis* / Q000235:genetics; D016695:Glycosyltransferases; D006168:Guinea Pigs; D000076002:Hyaluronan Synthases; D066298:In Vitro Techniques; D008297:Male; D008565:Membrane Proteins; D012333:RNA, Messenger / Q000096:biosynthesis; D014166:Transferases; D029867:Xenopus Proteins
D008565:Membrane Proteins; D012333:RNA, Messenger; D029867:Xenopus Proteins; D014166:Transferases; D016695:Glycosyltransferases; D014453:Glucuronosyltransferase; C056944:HAS1 protein, Xenopus; D000076002:Hyaluronan Synthases