Preservation of yeast cell morphology for scanning electron microscopy using 3.28-micro m IR laser irradiation.
As an alternative to conventional fixation procedures for scanning electron microscopy (SEM) analysis, yeast cells (Saccharomyces cerevisiae) were irradiated in ambient air, with an intense 3.28-micro m IR laser pulse. The morphology of the irradiated cells was well preserved, while nonirradiated control cells were severely shriveled.
Sunner
Jan
J
Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59717, USA. sunner@chemistry.montana.edu
Avci
Recep
R
Richards
Lee
L
Groenewold
Gary
G
Ingram
Jani
J
Arthun
Megan
M
D006046:Gold / Q000737:chemistry; D007834:Lasers; D008855:Microscopy, Electron, Scanning / Q000379:methods; D011309:Preservation, Biological; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000528:radiation effects / Q000648:ultrastructure*
D006046:Gold