title: Attachment of human marrow stromal cells to titanium surfaces.
abstract: The attachment of human bone marrow stromal cells to titanium alloy (Ti6Al4V) surfaces was investigated. Titanium disks were polished and modified by surface roughening and by passivation in nitric add. Cell attachment to titanium surfaces and tissue culture plastic (TCP) was determined by tetrazolium bromide (MTT) assay at 2, 6, 24, and 48 hours after seeding. Cell proliferation was determined by thymidine incorporation. Attachment on titanium surfaces was 75.6% to 94.9% of attachment on TCP control. The difference between cell attachment on the TCP compared with smooth or rough titanium was statistically significant (P < .05). However, no statistically significant difference was found between attachment to TCP and passivated titanium. Cell proliferation on titanium surfaces after 24 hours was approximately 70% of proliferation on TCP. There was a statistically significant difference (P < .05) between proliferation on tissue culture and smooth and passivated titanium but not on rough titanium. These results indicate that titanium provides a surface that is conducive to cell attachment and that passivating titanium improves cell attachment, approaching levels seen with TCP, a surface specifically developed to enhance cell attachment. Increasing surface roughness results in improved cell proliferation on titanium.
authors.affiliation: Department of Restorative Dentistry, School of Dental Medicine, University of Pennsylvania, 4001 Spruce Street, Philadelphia, PA 19104, USA.
authors.forename: Mamle
authors.initials: M
authors.lastname: Mante
authors.forename: Bina
authors.initials: B
authors.lastname: Daniels
authors.forename: Eleanor
authors.initials: E
authors.lastname: Golden
authors.forename: David
authors.initials: D
authors.lastname: Diefenderfer
authors.forename: Gwen
authors.initials: G
authors.lastname: Reilly
authors.forename: Phoebe S
authors.initials: PS
authors.lastname: Leboy
mesh_terms: D000497:Alloys; D001854:Bone Marrow Cells / Q000166:cytology*; D002448:Cell Adhesion; D002452:Cell Count; D018929:Cell Culture Techniques; D002455:Cell Division; D002470:Cell Survival; D004396:Coloring Agents; D003722:Dental Alloys / Q000737:chemistry*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D017942:Nitric Acid / Q000737:chemistry; D010969:Plastics / Q000737:chemistry; D017154:Stromal Cells / Q000166:cytology*; D013499:Surface Properties; D013778:Tetrazolium Salts; D013844:Thiazoles; D013997:Time Factors; D014025:Titanium / Q000737:chemistry*
chemical_list: D000497:Alloys; D004396:Coloring Agents; D003722:Dental Alloys; D010969:Plastics; D013778:Tetrazolium Salts; D013844:Thiazoles; C031462:titanium alloy (TiAl6V4); D017942:Nitric Acid; D014025:Titanium; C022616:thiazolyl blue