title: IGF-I augments resection-induced mucosal hyperplasia by altering enterocyte kinetics.
abstract: Our objective was to determine if exogenous insulin-like growth factor-I (IGF-I) augments the adaptive growth response to mid small bowel resection in association with changes in enterocyte kinetics. We determined structural adaptation and concomitant changes in enterocyte proliferation, apoptosis, and migration of the jejunum in growing, parenterally fed rats after mid small bowel resection or small bowel transection, and treatment with IGF-I or vehicle. IGF-I treatment in resected rats significantly increased jejunal mucosal mass by 20% and mucosal concentrations of protein and DNA by 36 and 33%, respectively, above the response to resection alone. The enhancement of resection-induced adaptive growth and cellularity by IGF-I reflected an increase in enterocyte proliferation, an expansion of the proliferative compartment in the crypt, and no further decrease in enterocyte apoptosis or increase in enterocyte migration beyond the effects of resection. The ability of IGF-I to augment the mucosal hyperplasia stimulated by the endogenous response to resection substantiates the role of IGF-I as an intestinal mitogen that promotes tissue regeneration.
authors.affiliation: Department of Nutritional Sciences, University of Wisconsin-Madison, Madison, WI 53706, USA.
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Dahly
authors.forename: Ziwen
authors.initials: Z
authors.lastname: Guo
authors.forename: Denise M
authors.initials: DM
authors.lastname: Ney
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001835:Body Weight; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D020895:Enterocytes / Q000166:cytology / Q000187:drug effects*; D006965:Hyperplasia; D018969:Insulin-Like Growth Factor Binding Proteins / Q000097:blood; D007334:Insulin-Like Growth Factor I / Q000378:metabolism / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000473:pathology* / Q000601:surgery*; D007583:Jejunum / Q000473:pathology* / Q000601:surgery*; D007700:Kinetics; D008297:Male; D009584:Nitrogen / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018969:Insulin-Like Growth Factor Binding Proteins; D007334:Insulin-Like Growth Factor I; D009584:Nitrogen