The beta1 integrin subunit is not a specific component of the costamere domain in human myocardial cells.
Studies on altered integrin receptor expression during cardiac hypertrophy and heart failure requires accurate knowledge of the distributional pattern of integrins in myocardial cells. At present the general consensus is that in cardiac muscle the beta1 integrin receptor is mainly localized to the same sarcolemmal domain as vinculin at Z-band levels ('costamere'). Since most previous studies have been focusing on myocardial integrin distribution in lower mammals, the myocardial localization of the beta1 integrin subunit was investigated in biopsies collected from the auricle of patients undergoing a coronary bypass operation. Non-invasive serial optical sectioning was carried out by immuno-laser scanning confocal microscopy. Double-labelling for vinculin/alpha-actinin, and the cytoplasmic domain for the beta1 integrin subunit, showed that beta1 integrin is deposited throughout both the vinculin/alpha-actinin domains and the non-vinculin/alpha-actinin domains. These results were supported by a semi-quantitative analysis in extended focus images of the latter preparations. Higher magnification views at the electron microscopical levels of the large, extracellular domain of the beta1 integrin subunit disclosed a pronounced labelling in the form of a dense, irregular punctuate pattern that was distributed at Z-disc domains as well as along the entire sarcolemmal area between Z-discs. Our findings show that in human, myocardial cells, the beta1 integrin receptor does not only localize to the surface membrane at the Z-disc level ('costamere' in cardiac muscle), but has a widespread distribution along the sarcolemma.
Saetersdal
Thorvald
T
Department of Anatomy and Cell Biology, Experimental Cardiology Unit, University of Bergen, 5021 Bergen, Norway.
Larsen
Terje H
TH
Dalen
Helge
H
D000911:Antibodies, Monoclonal; D006801:Humans; D007150:Immunohistochemistry; D019012:Integrin beta1 / Q000096:biosynthesis* / Q000276:immunology / Q000648:ultrastructure; D018613:Microscopy, Confocal; D016253:Microscopy, Immunoelectron; D032383:Myocytes, Cardiac / Q000276:immunology / Q000378:metabolism* / Q000648:ultrastructure; D021122:Protein Subunits; D012508:Sarcolemma / Q000276:immunology / Q000378:metabolism / Q000648:ultrastructure; D016596:Vinculin / Q000096:biosynthesis* / Q000276:immunology / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D019012:Integrin beta1; D021122:Protein Subunits; D016596:Vinculin