title: Comparative kinetic study of D-glucose oxidation by ruthenium(III) compounds catalyzed by FAD-dependent glucose oxidase and PQQ-dependent glucose dehydrogenase.
abstract: The comparative kinetic study of two glucose oxidizing enzymes, FAD-dependent glucose oxidase and PQQ-dependent glucose dehydrogenase, is presented in the artificial electron transfer mediator system based on ruthenium(III) compounds. It is demonstrated that FAD-dependent glucose oxidase and PQQ-dependent glucose dehydrogenase follow Michaelis kinetics in the D-glucose/ruthenium(III) system. PQQ-dependent glucose dehydrogenase is more active than FAD-dependent glucose oxidase in the process of D-glucose oxidation by ruthenium(III) compounds, this being due to the different catalytic mechanisms of these enzymes.
authors.affiliation: School of Chemistry, Lomonosov Moscow State University, Moscow, 119899, Russia. ivanka@enzyme.chem.msu.ru
authors.forename: E V
authors.initials: EV
authors.lastname: Ivanova
authors.forename: A Yu
authors.initials: AY
authors.lastname: Ershov
authors.forename: V
authors.initials: V
authors.lastname: Laurinavicius
authors.forename: R
authors.initials: R
authors.lastname: Meskus
authors.forename: A D
authors.initials: AD
authors.lastname: Ryabov
mesh_terms: D002384:Catalysis; D005947:Glucose / Q000737:chemistry* / Q000378:metabolism; D005948:Glucose Dehydrogenases / Q000737:chemistry* / Q000378:metabolism; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D015394:Molecular Structure; D010084:Oxidation-Reduction / Q000187:drug effects; D015363:Quinolones / Q000737:chemistry; D011809:Quinones / Q000737:chemistry; D012428:Ruthenium / Q000737:chemistry* / Q000494:pharmacology; D013237:Stereoisomerism; D013329:Structure-Activity Relationship; D013997:Time Factors
chemical_list: D015363:Quinolones; D011809:Quinones; D012428:Ruthenium; D005948:Glucose Dehydrogenases; D005949:Glucose Oxidase; D005947:Glucose