Low doses of diagnostic energy X-rays protect against neoplastic transformation in vitro.
OBJECTIVE
To investigate the effect of low doses of 60 kVp X-rays on in vitro transformation frequency.


METHODS
HeLa x skin fibroblast human hybrid cells were used to assay transformation from the non-tumorigenic to the tumorigenic phenotype. Subconfluent cultures of cells were exposed to a range of doses of 60 kVp X-rays and seeded for assay of transformation after 24 h post-irradiation holding. Experiments were repeated at least three times and the data pooled for analysis. Transformation frequencies were compared with those of sham-irradiated controls.


RESULTS
At doses < 1 cGy, the observed transformation frequencies were significantly less than those seen in unirradiated cells.


CONCLUSIONS
Low doses (< 1 cGy) of 60 kVp X-rays protect HeLa x skin fibroblast human hybrid cells against neoplastic transformation in vitro.
Redpath
J L
JL
Department of Radiation Oncology, College of Medicine, University of California Irvine, 92697, USA. jlredpat@uci.edu
Lu
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Lao
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Molloi
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Elmore
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D002471:Cell Transformation, Neoplastic / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006367:HeLa Cells; D006801:Humans; D006822:Hybrid Cells / Q000528:radiation effects; D066298:In Vitro Techniques; D009381:Neoplasms, Radiation-Induced / Q000209:etiology; D012307:Risk Factors; D014965:X-Rays