Lipophilic derivatization of synthetic peptides belonging to NS3 and E2 proteins of GB virus-C (hepatitis G virus) and its effect on the interaction with model lipid membranes.
The synthesis by solid-phase methodologies of peptides belonging to structural and non-structural proteins of GB virus C as well as its N-alpha-acylation with myristate and palmitate fatty acids is described. To explore the peptide-lipid interactions we have used liposomes composed of dipalmitoylphosphatidylcholine as model membranes and complementary spectroscopic and calorimetric techniques. Our results show that structural and more clearly the structural lipophilic peptide sequences incorporated into lipid bilayers perturb the packing of lipids and affect their thermotropic properties, more than the non-structural selected sequence. However, the binding of the synthetic sequences to lipid membranes occurred without any restructuration of the peptides.
Rojo
N
N
Department of Peptide and Protein Chemistry, IIQAB-CSIC, Barcelona, Spain.
Gómara
M J
MJ
Alsina
M A
MA
Haro
I
I
D000595:Amino Acid Sequence; D002152:Calorimetry, Differential Scanning; D002462:Cell Membrane / Q000187:drug effects*; D002942:Circular Dichroism; D005453:Fluorescence; D007526:Isoelectric Point; D008081:Liposomes / Q000378:metabolism*; D008956:Models, Chemical; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D020365:RNA Helicases; D012697:Serine Endopeptidases; D012995:Solubility; D013816:Thermodynamics; D014759:Viral Envelope Proteins / Q000737:chemistry*; D017361:Viral Nonstructural Proteins / Q000737:chemistry*; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
D008081:Liposomes; D010455:Peptides; D020365:RNA Helicases; D012697:Serine Endopeptidases; D014759:Viral Envelope Proteins; D017361:Viral Nonstructural Proteins; C060028:NS3 protein, flavivirus; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases; C107619:glycoprotein E2, GB virus C