title: UV wavelength-dependent regulation of transcription-coupled nucleotide excision repair in p53-deficient human cells.
abstract: Nucleotide excision repair (NER) prevents skin cancer by eliminating highly genotoxic cyclobutane pyrimidine dimers (CPDs) induced in DNA by the UVB component of sunlight. NER consists of two distinct but overlapping subpathways, i.e., global NER, which removes CPD from the genome overall, and transcription-coupled NER (TCNER), which removes CPD uniquely from the transcribed strand of active genes. Previous investigations have clearly established that the p53 tumor suppressor plays a crucial role in the NER process. Here we used the ligation-mediated PCR technique to demonstrate, at nucleotide resolution along two chromosomal genes in human cells, that the requirement for functional p53 in TCNER, but not in global NER, depends on incident UV wavelength. Indeed, relative to an isogenic p53 wild-type counterpart, p53-deficient human lymphoblastoid strains were shown to remove CPD significantly less efficiently along both the transcribed and nontranscribed strands of the c-jun and hprt loci after exposure to polychromatic UVB (290-320 nm). However, in contrast, after irradiation with 254-nm UV, p53 deficiency engendered less efficient CPD repair only along the nontranscribed strands of these target genes. The revelation of this intriguing wavelength-dependent phenomenon reconciles an apparent conflict between previous studies which used either UVB or 254-nm UV to claim, respectively, that p53 is required for, or plays no role whatsoever in, TCNER of CPD. Furthermore, our finding highlights a major caveat in experimental photobiology by providing a prominent example where the extensively used "nonsolar" model mutagen 254-nm UV does not accurately replicate the effects of environmentally relevant UVB.
authors.affiliation: Faculty of Medicine, University of Montréal, Montréal, PQ, Canada H3C 3J7.
authors.forename: Geraldine
authors.initials: G
authors.lastname: Mathonnet
authors.forename: Caroline
authors.initials: C
authors.lastname: Leger
authors.forename: Julie
authors.initials: J
authors.lastname: Desnoyers
authors.forename: Regen
authors.initials: R
authors.lastname: Drouin
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Therrien
authors.forename: Elliot A
authors.initials: EA
authors.lastname: Drobetsky
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D004247:DNA / Q000235:genetics; D004260:DNA Repair / Q000235:genetics / Q000502:physiology / Q000528:radiation effects*; D016158:Genes, p53; D006801:Humans; D018462:Photobiology; D012878:Skin Neoplasms / Q000517:prevention & control; D014158:Transcription, Genetic; D016159:Tumor Suppressor Protein p53 / Q000172:deficiency*; D014466:Ultraviolet Rays
chemical_list: D016159:Tumor Suppressor Protein p53; D004247:DNA