[Cloning and expression of maize fructose-6-phosphate, 2-kinase/fructose-2,6-bisphosphatase (mF2KP)].
A 2469 bp cDNA encoding entire fructose-6-phosphate, 2-kinase/fructose-2,6-bisphosphatase (F2KP) was cloned from maize (Zea mays L.cv. Ziyunuo 1) by the methods of RT-PCR and rapid amplification of cDNA ends(RACE), on the basis of AF007582, which was isolated from "Yedan 4". The cDNA was designated as mF2KP and the GenBank accession number is AF334143, which contains a 2226 bp open reading from (ORF), encoding a 741 residue polypeptide. There are some differences between the F2KP genes of two maize varieties. The length of 3' non-coding region of mF2KP is 38 bp shorter than that of AF007582. On the 1592th, 1593th and 1605th positions of mF2KP, there is an additional nucleotide respectively compared with AF007582, which cause a shifted reading frame in a small region. Northern blot showed that the expression of mF2KP were significantly different among maize tissues. The Transcription of mF2KP in stem was lower than those in leaves, kernel leaves and male inflorescence, but much higher than that in immature seeds.
Wang
Dong
D
National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.
Du
Xi-Ling
XL
Hu
Jian-Guang
JG
Zhang
Hong-Sheng
HS
Yang
Jin-Shui
JS
Zhai
Hu-Qu
HQ
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D025481:Phosphofructokinase-2 / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D003313:Zea mays / Q000201:enzymology / Q000235:genetics*
D018076:DNA, Complementary; D018749:RNA, Plant; D025481:Phosphofructokinase-2