[Determination of nucleosides in siweilingzhi mixture by HPCE].
OBJECTIVE
To establish a method for determining nucleosides (adenoside and guanoside) in Siweilingzhi Mixture by HPCE.


METHODS
Adenoside and guanoside were separated within 25 min using an 20 mmol.L-1 borate buffer with 30 mmol.L-1 SDS and 5% Ethanol (adjusted to pH 10.0 with sodium hydroxide solution), with an operation voltage of 10 kV, temperature of 20 degrees C and a hydrodynamic injection time of 15 s. Seperations were carried out in a fused-silica capillary 75 microns id x 57 cm (effective length 50 cm) with peak detection by direct UV at 254 nm.


RESULTS
Regression equation of adenoside and that of guanoside were Y = 0.0705 + 0.01707X (r = 0.9995) and Y = 0.0232 + 0.01864X (r = 0.9999) respectively. The average recovery rate was 99.22% (RSD = 3.66%) and 104.3% (RSD = 1.91%) respectively. Nine samples were determined with the method.


CONCLUSIONS
The method is simple, rapid and accurate with good repeatability and it can be used to determine nucleosides.
Dai
Jing
J
Hebei Provincial Institute for Drug Control, Shijiazhuang 050011, Hebei, China.
Lu
Jing
J
Lin
Rui-chao
RC
Liu
Wen-ying
WY
D000241:Adenosine / Q000032:analysis*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification; D019075:Electrophoresis, Capillary / Q000379:methods; D005285:Fermentation; D006151:Guanosine / Q000032:analysis*; D020940:Reishi / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D006151:Guanosine; D000241:Adenosine