[The effects of conjugated linoleic acid on the expression of invasiveness and metastasis-associated gene of human gastric carcinoma cell line].
OBJECTIVE
To study the effects of c9,t11-conjugated linoleic acid (c9,t11-CLA) on invasive ability of human gastric carcinoma cell line (SGC-7901) and to explore its possible mechanism.


METHODS
Reconstituted basement membrane invasion assay was used to evaluate invasive ability of cancer cells. Expression of TIMP-1, TIMP-2 and nm23-H(1) mRNA was measured by reverse transcription polymerase chain reaction (RT-PCR) in SGC-7901 cells.


RESULTS
At the concentrations of 200 micromol/L, 100 micromol/L and 50 micromol/L, c9,t11-CLA suppressed their reconstituted basement membrane invasion of SGC-7901 by 53.7%, 40.9% and 29.3%, respectively. c9,t11-CLA could induce the expression of TIMP-1, TIMP-2 and nm23-H(1) mRNA in SGC-7901 cells.


CONCLUSIONS
The invasion of SGC-7901 cells could be inhibited by c9,t11-CLA through reconstituted basement membrane. Anti-invasion action of c9,t11-CLA might be associated with induction of expression of TIMP-1, TIMP-2 and nm23-H(1) mRNA in tumor cells.
Yang
Yan-mei
YM
Department of Nutrition and Food Hygiene, Public Health School of Harbin Medical University, Harbin 150001, China.
Chen
Bing-qing
BQ
Zheng
Yu-mei
YM
Wang
Xuan-lin
XL
Liu
Jia-ren
JR
Xue
Ying-ben
YB
Liu
Rui-hai
RH
D000230:Adenocarcinoma / Q000473:pathology; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D019787:Linoleic Acid / Q000494:pharmacology* / Q000627:therapeutic use; D020559:Monomeric GTP-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D054778:NM23 Nucleoside Diphosphate Kinases; D009361:Neoplasm Invasiveness / Q000517:prevention & control; D009701:Nucleoside-Diphosphate Kinase; D012333:RNA, Messenger / Q000096:biosynthesis / Q000187:drug effects; D013274:Stomach Neoplasms / Q000473:pathology; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000096:biosynthesis / Q000235:genetics; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000096:biosynthesis / Q000235:genetics; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics; D014407:Tumor Cells, Cultured
D054778:NM23 Nucleoside Diphosphate Kinases; D012333:RNA, Messenger; D019715:Tissue Inhibitor of Metalloproteinase-1; D014157:Transcription Factors; D019716:Tissue Inhibitor of Metalloproteinase-2; D019787:Linoleic Acid; C517938:NME1 protein, human; D009701:Nucleoside-Diphosphate Kinase; D020559:Monomeric GTP-Binding Proteins