Differential effects of quercetin and resveratrol on Band 3 tyrosine phosphorylation signalling of red blood cells.
The protective effects of eating fruits and vegetables in the prevention of several degenerative pathologies have been attributed at least in part to the antioxidant and anti-inflammatory properties of polyphenols. In this study, we investigated the effects of two polyphenols, quercetin and resveratrol, on red blood cell Band 3 tyrosine phosphorylation signalling activated by peroxynitrite. Peroxynitrite is a physiological oxidant scavenged largely by the erythrocyte and formed by the reaction between nitrogen monoxide and superoxide anion. Quercetin and its structurally analogous (+)-catechin inhibited the peroxynitrite-dependent upregulation of Band 3 tyrosine phosphorylation. Quercetin was found to downregulate the activity of syk, which is upstream in the Band 3 tyrosine phosphorylation cascade, and partially prevented peroxynitrite-mediated phosphotyrosine phosphatase inhibition. Resveratrol and hydroxytyrosol, unexpectedly, amplified peroxynitrite-dependent upregulation of Band 3 tyrosine phosphorylation through the activation of lyn, a kinase of the src family. The present results clearly indicate that polyphenols may activate cell transduction pathways in different and sometimes opposite ways.
Maccaglia
Alessandro
A
Cell Biology Department, Istituto Superiore di Sanità, Viale Regina Elena, 299-00161 Rome, Italy.
Mallozzi
Cinzia
C
Minetti
Maurizio
M
D001457:Anion Exchange Protein 1, Erythrocyte / Q000737:chemistry / Q000378:metabolism*; D000975:Antioxidants / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004792:Enzyme Precursors / Q000187:drug effects / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000201:enzymology; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D030421:Peroxynitrous Acid / Q000037:antagonists & inhibitors / Q000494:pharmacology; D010766:Phosphorylation; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000187:drug effects / Q000378:metabolism; D011794:Quercetin / Q000494:pharmacology*; D000077185:Resveratrol; D015398:Signal Transduction / Q000187:drug effects; D013267:Stilbenes / Q000494:pharmacology*; D000072377:Syk Kinase; D014443:Tyrosine / Q000378:metabolism*; D019061:src-Family Kinases / Q000187:drug effects / Q000378:metabolism
D001457:Anion Exchange Protein 1, Erythrocyte; D000975:Antioxidants; D004792:Enzyme Precursors; D047908:Intracellular Signaling Peptides and Proteins; D013267:Stilbenes; D030421:Peroxynitrous Acid; D014443:Tyrosine; D011794:Quercetin; D011505:Protein-Tyrosine Kinases; C000606896:SYK protein, human; D000072377:Syk Kinase; C060902:lyn protein-tyrosine kinase; D019061:src-Family Kinases; D017027:Protein Tyrosine Phosphatases; D000077185:Resveratrol