title: Simple and rapid chromatographic purification of type V collagen from a pepsin digest of porcine intestinal connective tissue, an unmanageable starting material for conventional column chromatography.
abstract: A chromatographic method is described for purification of type V collagen, a minor constituent in extracellular matrix, from a pepsin digest of porcine intestinal connective tissue. The starting material was a viscous and turbid solution even after centrifugation. Direct application of the sample to a commercially available DEAE-cellulose column resulted in clogging. On the other hand, type V collagen, [alpha1(V)](2)alpha2(V) form, was successfully captured by a filter paper-based DEAE-cellulose column chromatography and purified by a subsequent commercially available cation-exchange medium without clogging. This is a vast improvement over previously described salt fractionation methods.
authors.affiliation: Department of Food Sciences and Nutritional Health, Kyoto Prefectural University, Shimogamao, 606-8522, Kyoto, Japan. k_sato@kpu.ac.jp
authors.forename: Kenji
authors.initials: K
authors.lastname: Sato
authors.forename: Tamae
authors.initials: T
authors.lastname: Tanahashi-Shiina
authors.forename: Feng
authors.initials: F
authors.lastname: Jun
authors.forename: Atsuko
authors.initials: A
authors.lastname: Watanabe-Kawamura
authors.forename: Masami
authors.initials: M
authors.lastname: Ichinomiya
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Minegishi
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Tsukamasa
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Makoto
authors.initials: M
authors.lastname: Kawabata
authors.forename: Kozo
authors.initials: K
authors.lastname: Ohtsuki
mesh_terms: D000818:Animals; D002848:Chromatography, DEAE-Cellulose / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D024062:Collagen Type V / Q000302:isolation & purification*; D003238:Connective Tissue / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D010434:Pepsin A / Q000378:metabolism*; D013552:Swine
chemical_list: D024062:Collagen Type V; D010434:Pepsin A