title: Multilocus sequence typing (MLST) of Escherichia coli O78 strains.
abstract: Strains of Escherichia coli serotype O78 are associated with many diseases, including invasive infections, in humans and farm animals. The clonal relationship between strains from different hosts is therefore important for assessing the risk of zoonotic infections. Here we propose a multilocus sequence typing scheme for E. coli, based on six housekeeping genes. Preliminary, but significant, results indicate that clonal division in E. coli O78 strains is host independent, and closely related clones reside in different hosts. There was a positive correlation between virulence and clonal origin.
authors.affiliation: Department of Molecular Microbiology and Biotechnology, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv, Israel.
authors.forename: Roni S
authors.initials: RS
authors.lastname: Adiri
authors.forename: Uri
authors.initials: U
authors.lastname: Gophna
authors.forename: Eliora Z
authors.initials: EZ
authors.lastname: Ron
mesh_terms: D000217:Acyltransferases / Q000235:genetics; D000263:Adenylate Kinase / Q000235:genetics; D000483:Alleles; D000818:Animals; D000829:Animals, Domestic; D002262:Carboxy-Lyases / Q000235:genetics; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology*; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics; D051082:Homoserine O-Succinyltransferase; D006801:Humans; D008291:Malate Dehydrogenase / Q000235:genetics; D017856:Phosphotransferases (Phosphate Group Acceptor) / Q000235:genetics; D010802:Phylogeny; D011110:Polymorphism, Genetic; D018805:Sepsis / Q000382:microbiology*; D012703:Serotyping; D014774:Virulence; D015047:Zoonoses
chemical_list: D008291:Malate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D000217:Acyltransferases; D051082:Homoserine O-Succinyltransferase; D017856:Phosphotransferases (Phosphate Group Acceptor); C022424:polyphosphate kinase; D000263:Adenylate Kinase; D002262:Carboxy-Lyases; C022713:tartronate-semialdehyde synthase