title: Absence of evidence for metabolite-modulated association between alpha-glycerol-3-phosphate dehydrogenase and L-lactate dehydrogenase.
abstract: Evidence for the NADH-modulated formation of a complex between alpha-glycerol-3-phosphate dehydrogenase and l-lactate dehydrogenase was reported [Yong, H., Thomas, G. A., and Peticolas, W. L. (1993) Biochemistry 32, 11124-11131]. This NADH-modulated association suggested a mechanism of potentially great importance to enzyme modulation and the controversial phenomena of direct NADH channeling. In the present paper, we reproduce with additional controls the experiments described by Yong et al. ((1993) Biochemistry 32, 11124-11131). Our results conclusively demonstrate the absence of detectable association between alpha-glycol-3-phosphate dehydrogenase and l-lactate dehydrogenase.
authors.affiliation: Department of Biochemistry and Molecular Biology, Noble Research Center, Oklahoma State University, Stillwater 74078, USA. elehoux@biochem.okstate.edu
authors.forename: Eric A
authors.initials: EA
authors.lastname: Lehoux
authors.forename: Sharon M
authors.initials: SM
authors.lastname: Baker
authors.forename: Marina V
authors.initials: MV
authors.lastname: Kovina
authors.forename: Franklin A
authors.initials: FA
authors.lastname: Hays
authors.forename: H Olin
authors.initials: HO
authors.lastname: Spivey
mesh_terms: D000818:Animals; D002850:Chromatography, Gel / Q000379:methods; D005993:Glycerolphosphate Dehydrogenase / Q000737:chemistry / Q000378:metabolism*; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000737:chemistry / Q000378:metabolism*; D046911:Macromolecular Substances; D009243:NAD / Q000737:chemistry / Q000378:metabolism; D011817:Rabbits; D034421:Sus scrofa
chemical_list: D046911:Macromolecular Substances; D009243:NAD; D005993:Glycerolphosphate Dehydrogenase; D007770:L-Lactate Dehydrogenase