Biosynthesis of isotopically labeled gramicidins and tyrocidins by Bacillus brevis.
The three-dimensional structure of bilayer-associated gramicidin A is available from a structural data base. This and related peptides are, therefore, ideal model compounds to use during the implementation and development of new NMR techniques for the structural investigations of membrane proteins. As these methods rely on the isotopic labelling of single, selected or all sites, we have, investigated and optimised biochemical protocols using different strains of the Gram-positive bacterium Bacillus brevis. With newly developed schemes for isotopic labelling large amounts of gramicidin and tyrocidin enriched with stable isotopes such as (15)N or (15)N/(13)C have been obtained at low cost. A variety of analytical and spectroscopic techniques, including HPLC, mass spectrometry and NMR spectroscopy are used to characterise the resulting products.
Vogt
T C Bas
TC
Max-Planck-Institut für Biochemie, Am Klopferspitz 18A, 82152 Martinsried, Germany.
Schinzel
Susan
S
Bechinger
Burkhard
B
D000595:Amino Acid Sequence; D001407:Bacillus / Q000378:metabolism*; D002247:Carbon Isotopes; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006096:Gramicidin / Q000096:biosynthesis* / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009587:Nitrogen Isotopes; D014440:Tyrocidine / Q000096:biosynthesis* / Q000737:chemistry
D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D014440:Tyrocidine; D006096:Gramicidin