Clathrin-coated vesicle formation: a paradigm for coated-vesicle formation.
Clathrin-coated pits are the major ports of entry into the cell and are responsible for the internalization of a variety of biologically important macromolecules. These transport intermediates form as a result of the co-ordinated assembly of a number of cytosolic proteins on to the membrane which results in specific cargo recruitment. We have used a variety of approaches including permeabilized cell assays and light and electron microscopy to identify and characterize the proteins and enzymes involved in coated vesicle formation.
Smythe
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Centre for Developmental Genetics, Department of Biomedical Sciences, University of Sheffield, Sheffield S10 2TN, UK. e.symthe@sheffield.ac.uk
D000818:Animals; D002966:Clathrin / Q000502:physiology; D022163:Clathrin-Coated Vesicles / Q000502:physiology*; D004705:Endocytosis; D008954:Models, Biological
D002966:Clathrin