title: [Effect of erythromycin on apoptosis of eosinophils in nasal polyps in vitro].
abstract: OBJECTIVE
  To study the effect of erythromycin on apoptosis of eosinophils in nasal polyps in vitro.
  
  
  METHODS
  Fifteen nasal polyp biopsies were collected during nasal polypectomy. The specimens were cultured with erythromycin in RPMI1640, and cultured without erythromycin as control group. The explants in both groups were taken out at 1, 3, 5 day after culture. The apoptosis of cells was detected by terminal deoxynucleotidyl transferasemediated dUTP nick endlabeling(TUNEL), eosinophils were identified by morphology and serial sections stained with HE, then the apoptosis index (AI) of eosinophils was calculated.
  
  
  RESULTS
  The AI of eosinophils in nasal polyps after cultured for 1,3,5 days with erythromycin were (31.77 +/- 9.52)%, (32.67 +/- 9.44)% and (50.34 +/- 8.78)% respectively. In control group, the AI of eosinophils were (29.18 +/- 7.31)%, (29.82 +/- 12.03)% and (42.25 +/- 8.08)% respectively. There were significant difference between two groups at 5 day after culture(P < 0.05).
  
  
  CONCLUSIONS
  Erythromycin promoted apoptosis of eosinophils in nasal polyps in vitro.
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China. tjent@163.net
authors.forename: Jun
authors.initials: J
authors.lastname: Tang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.forename: Geng
authors.initials: G
authors.lastname: Xu
authors.forename: Rui
authors.initials: R
authors.lastname: Xu
authors.forename: Gehua
authors.initials: G
authors.lastname: Zhang
authors.forename: Xian
authors.initials: X
authors.lastname: Liu
mesh_terms: D000293:Adolescent; D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002648:Child; D004804:Eosinophils / Q000187:drug effects*; D004917:Erythromycin / Q000494:pharmacology*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000473:pathology*
chemical_list: D000900:Anti-Bacterial Agents; D004917:Erythromycin