title: The medaka fish Tol2 transposable element can undergo excision in human and mouse cells.
abstract: Tol2 is an active DNA-based transposable element identified in the medaka fish, Oryzias latipes. Originating from a vertebrate and belonging to the hAT ( hobo/ Activator/ Tam3) transposable element family, featuring a wide distribution among organisms, Tol2 would be expected to be active if introduced into mammals. We, therefore, examined if excision, one part of the transposition reaction, can occur in human and mouse culture cells. A Tol2 clone was introduced into cells and, after incubation, recovered. PCR and sequencing analysis provided evidence for precise and near precise excision in these cells. Tol2 can thus be expected to serve as a material for developing a gene transfer vector and other genetic tools applicable to mammals. It was also suggested that an intact Tol2 element could retain autonomy as a transposable element in mammalian cells.
authors.affiliation: Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya 464-8602, Japan. koga@bio.nagoya-u.ac.jp.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Koga
authors.affiliation: Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya 464-8602, Japan.
authors.forename: Atsuo
authors.initials: A
authors.lastname: Iida
authors.affiliation: Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya 464-8602, Japan.
authors.forename: Megumi
authors.initials: M
authors.lastname: Kamiya
authors.affiliation: Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya 464-8602, Japan.
authors.forename: Ryoko
authors.initials: R
authors.lastname: Hayashi
authors.affiliation: Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya 464-8602, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hori
authors.affiliation: National Institute of Radiological Sciences, Chiba, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Ishikawa
authors.affiliation: Radiation Biology Center, Kyoto University, Kyoto, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Tachibana
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D004251:DNA Transposable Elements / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009990:Oryzias / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D004251:DNA Transposable Elements