[Survivin mutants reverse the malignancy of HeLa cells].
OBJECTIVE
Survivin was aberrantly expressed in most cancer tissues, suggesting that survivin plays an important role in carcinogenesis. This study was designed to investigate the function and mechanism of survivin mutants in tumor cells.


METHODS
The site-mutant and truncated survivin mutants were transfected into HeLa cells and selected using G418. Cell apoptosis was analyzed using flow cytometry. Protein level of cyclin D1 was detected by Western blot analysis.


RESULTS
Survivin mutant plasmid expressed in the HeLa cells successfully. The expressed protein could be detected using related antibody. Colony formation ability significantly decreased in the HeLa cells with survivin mutants compared with that in the parental HeLa cells. The HeLa cells transfected instantly with survivin mutants could undergo apoptosis automatically. Meanwhile, survivin mutants could cause an increase of multinuclear HeLa cells. The effect of survivin-N showed more effective than that of survivin T34A. Survivin-N and survivin T34A could influence the expression of cyclin D1 and reduced its protein levels of 68% and 12%, respectively.


CONCLUSIONS
Survivin mutants can partially reverse the malignancy of HeLa cells. The reduction of cyclin D1 induced by survivin mutants may play an important role in it. Survivin may be a target gene in gene therapy of cancer.
Zhu
Hong-Xia
HX
Laboratory of Cell and Molecular Biology, Cancer Institute, Chinese Academy of Medical Science, Peking Union Medical College, Beijing, PR China.
Zhou
Cui-Qi
CQ
Zhang
Guo
G
Zhou
Xiao-Bo
XB
Liu
Shuang
S
Bai
Jin-Feng
JF
Quan
Lan-Ping
LP
Dong
Zhi-Wei
ZW
Xu
Ning-Zhi
NZ
D017209:Apoptosis / Q000502:physiology*; D019938:Cyclin D1 / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000502:physiology*; D009154:Mutation; D009363:Neoplasm Proteins; D000077022:Survivin; D014162:Transfection; D014407:Tumor Cells, Cultured
C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D000077022:Survivin; D019938:Cyclin D1