title: Sequence analysis demonstrates that Onion yellow dwarf virus isolates from China contain a P3 region much larger than other potyviruses.
abstract: The complete sequence of an isolate of Onion yellow dwarf virus (OYDV) from Yuhang, Zhejiang province, China, was determined. It was 10538 nts in length and was predicted to encode a polyprotein 3403 amino acids (aa) long with a calculated M(r) of 385.1 kDa. The predicted P3 protein (530 aa) was larger than that of any of the potyviruses sequenced to date (344-378 aa). The additional sequence occurs at the N-terminus of the protein, does not represent a duplication from elsewhere in the OYDV genome and could not be matched to any other sequences in the databases. Similar sequences were found in 4 other Chinese OYDV isolates. Phylogenetic analysis of the amino acid sequences of the polyprotein showed that OYDV is distantly related to Pea seed-borne mosaic virus and the potyviruses of grasses and cereals.
authors.affiliation: Virology and Biotechnology Department, Zhejiang Academy of Agricultural Sciences, Hangzhou, People's Republic of China.
authors.forename: J
authors.initials: J
authors.lastname: Chen
authors.forename: M J
authors.initials: MJ
authors.lastname: Adams
authors.forename: H-Y
authors.initials: HY
authors.lastname: Zheng
authors.forename: J-P
authors.initials: JP
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D002681:China; D008969:Molecular Sequence Data; D019697:Onions / Q000821:virology*; D010802:Phylogeny; D019176:Potyviridae / Q000145:classification* / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D014764:Viral Proteins