title: In vivo distribution of receptor for ecotropic murine leukemia virus and binding of envelope protein of Friend Murine leukemia virus.
abstract: Ecotropic infection by Murine leukemia virus (MuLV) infection is initiated by the interaction between the receptor-binding domain of the viral surface glycoprotein (SU) and the cell-surface receptor, mCAT-1. To study the in vivo localization of viral binding site in mice, green fluorescence protein (GFP)-tagged Friend SU (F-SU/GFP) was incubated with tissue sections. Lymphohematopoietic organs and a part of the glandular tissues of C3H as well as C57BL/6 mice revealed positive signals for F-SU/GFP binding on the cell surface. In contrast, C4W mice, which is a partial congenic mouse strain carrying the Fv-4 (r) gene on a BALB/c genetic background, exhibited negative signals in most of the organs except for a very weak binding in the pancreas. The expression of mCAT-1 mRNA determined by reverse transcriptase (RT)-polymerase chain reaction (PCR) revealed a similar distribution in C3H, C57BL/6 and C4W mice. Most of the organs including lymphohematopoietic organs and glandular organs revealed significant expression of mRNA for mCAT-1 gene, while the liver, heart and muscle did not. The results from binding assay were consistent with the fact that Friend MuLV-induced pathogenesis was usually associated with lymphohematopoietic systems, although mRNA expression for mCAT-1 was rather ubiquitous. The discrepancy between F-SU/GFP binding and mRNA expression for mCAT-1 in lymphohematopoietic organs of C4W mice would support the receptor interference effect by the Fv-4 (r) gene causing the resistance of C4W mouse to Friend MuLV infection.
authors.affiliation: Department of Pathology and Immunology, Aging and Developmental Sciences, Tokyo Medical and Dental University, Graduate School, Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Yamaguchi
authors.forename: M
authors.initials: M
authors.lastname: Hasegawa
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.forename: H
authors.initials: H
authors.lastname: Ikeda
authors.forename: S
authors.initials: S
authors.lastname: Aizawa
authors.forename: K
authors.initials: K
authors.lastname: Hirokawa
authors.forename: M
authors.initials: M
authors.lastname: Kitagawa
mesh_terms: D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000378:metabolism; D027201:Cationic Amino Acid Transporter 1 / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D005622:Friend murine leukemia virus / Q000737:chemistry / Q000378:metabolism*; D015870:Gene Expression; D007668:Kidney / Q000378:metabolism; D009052:Leukemia Virus, Murine / Q000378:metabolism*; D008168:Lung / Q000378:metabolism; D008198:Lymph Nodes / Q000378:metabolism; D051379:Mice; D010179:Pancreas / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D011991:Receptors, Virus / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D012469:Salivary Glands / Q000378:metabolism; D013154:Spleen / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000378:metabolism*
chemical_list: D027201:Cationic Amino Acid Transporter 1; D012333:RNA, Messenger; D011991:Receptors, Virus; C420826:Slc7a1 protein, mouse; D014759:Viral Envelope Proteins