[A comparative study on sterols of ethanol extract and water extract from Hericium erinaceus].
OBJECTIVE
To study the pharmaceutic chemical basis of the different medicinal effects, and to compare and analyze the sterols in Hericium erinaceus mycelia and its ethanol extract, water extract derived from solid fermented mycelia.


METHODS
The components of Hericium erinaceus mycelia and two kinds of extract were compared with some biochemical methods such as GC, RP-HPLC, etc. Sterol composition in Hericium erinaceus mycelia was determined by using GC-mass spectrometry.


RESULTS
The content of crude polysaccharide, water-soluble protein and fatty acid of ethanol extract showed no obvious advantage over its water extract. However, there was significant difference between two kinds of extract in the content of sterols. And ergostane derivatives, beta-sitosterol and C28 sterol with four bonds were detected as the major sterols in Hericium erinaceus mycelia, among which ergosterol was the principal sterol.


CONCLUSIONS
Sterols in Hericium erinaceus exist mainly in ethanol extract. And three sterols are found for the first time from Hericium erinaceus, which are ergostane derivatives, beta-sitosterol and C28 sterol with four bonds.
Li
J L
JL
College of Life Sciences, Nanjing Normal University, Nanjing 210097, Jiangsu, China.
Lu
L
L
Dai
C C
CC
Chen
K
K
Qiu
J Y
JY
D001487:Basidiomycota / Q000737:chemistry*; D004875:Ergosterol / Q000032:analysis*; D000431:Ethanol; D025282:Mycelium / Q000737:chemistry; D014867:Water
D014867:Water; D000431:Ethanol; D004875:Ergosterol