title: [Proteomics-based identification of Maspin differential expression in bronchial epithelial immortalized cells and malignant transformation cells].
abstract: OBJECTIVE
  Maspin, a serepin inhibitor, plays a key role in tumor growth and metastasis. The aim of this study was to identify the differential expression of Maspin in malignant transformation process of bronchial epithelial cells by proteomics.
  
  
  METHODS
  Functional proteomics analysis of Maspin on bronchial epithelial immortalized cells and malignant transformation cells was carried out using immobilized pH gradient (IPG) two-dimensional electrophoresis, peptide mass fingerprinting (PMF), and post source decay (PSD) of bio-mass spectrometry.
  
  
  RESULTS
  Nearly 1500 expressed proteins profile on bronchial epithelial immortalized cells and malignant transformation cells were obtained in the range of MW 14.4-94 kDa, PI 3-10. Image analysis showed that Maspin was down-regulated in malignant transformation cells compared with that in immortalized cells. Northern blot analysis showed that the mRNA abundance of Maspin in malignant transformation cells was much lower than that in immortalized cells.
  
  
  CONCLUSIONS
  Alteration expression of Maspin at transcription and translation levels might be involved in carcinogenesis of lung.
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medicine, Beijing, PR China.
authors.forename: Ling
authors.initials: L
authors.lastname: Xie
authors.forename: Wan-Tao
authors.initials: WT
authors.lastname: Ying
authors.forename: Bang-Yin
authors.initials: BY
authors.lastname: Li
authors.forename: Kai-Tai
authors.initials: KT
authors.lastname: Zhang
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Qian
authors.forename: Xiao-Qiong
authors.initials: XQ
authors.lastname: Xiang
authors.forename: De-Chang
authors.initials: DC
authors.lastname: Wu
mesh_terms: D015152:Blotting, Northern; D001980:Bronchi / Q000166:cytology; D002461:Cell Line, Transformed / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D004847:Epithelial Cells / Q000378:metabolism*; D020869:Gene Expression Profiling; D016147:Genes, Tumor Suppressor; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D015843:Serpins / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D011506:Proteins; C085344:SERPIN-B5; D015843:Serpins