Structure of frataxin iron cores: an X-ray absorption spectroscopic study.
X-ray absorption spectroscopy at the iron K-edge indicates that the iron cores of human and yeast frataxin polymers assembled in vitro are identical to each other and are similar but not identical to ferritin cores. Both frataxin polymers contain ferrihydrite, a biomineral composed of ferric oxide/hydroxide octahedra. The ferrihydrite in frataxin is less ordered than iron cores of horse spleen ferritin, having fewer face-sharing Fe-Fe interactions but similar double corner-sharing interactions. The extended X-ray absorption fine structure (EXAFS) analysis agrees with previous electron microscopy data showing that frataxin cores are composed of very small ferrihydrite crystallites.
Nichol
Helen
H
Department of Anatomy and Cell Biology, University of Saskatchewan, Saskatoon, Canada S7N 5E5. h.nichol@usask.ca
Gakh
Oleksandr
O
O'Neill
Heather A
HA
Pickering
Ingrid J
IJ
Isaya
Grazia
G
George
Graham N
GN
D000818:Animals; D005293:Ferritins / Q000737:chemistry; D006736:Horses; D006801:Humans; D007501:Iron / Q000737:chemistry / Q000378:metabolism; D033862:Iron-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D015394:Molecular Structure; D011994:Recombinant Proteins / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism; D013045:Species Specificity; D013057:Spectrum Analysis; D014965:X-Rays; D000096923:Frataxin
D005293:Ferritins; D007501:Iron; D033862:Iron-Binding Proteins; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D000096923:Frataxin