title: Notch2 is preferentially expressed in mature B cells and indispensable for marginal zone B lineage development.
abstract: The Notch genes play a key role in cellular differentiation. The significance of Notch1 during thymocyte development is well characterized, but the function of Notch2 is poorly understood. Here we demonstrate that Notch2 but no other Notch family member is preferentially expressed in mature B cells and that conditionally targeted deletion of Notch2 results in the defect of marginal zone B (MZB) cells and their presumed precursors, CD1d(hi) fraction of type 2 transitional B cells. Among Notch target genes, the expression level of Deltex1 is prominent in MZB cells and strictly dependent on that of Notch2, suggesting that Deltex1 may play a role in MZB cell differentiation.
authors.affiliation: Department of Hematology and Oncology, Graduate School of Medicine, University of Tokyo, Japan.
authors.forename: Toshiki
authors.initials: T
authors.lastname: Saito
authors.forename: Shigeru
authors.initials: S
authors.lastname: Chiba
authors.forename: Motoshi
authors.initials: M
authors.lastname: Ichikawa
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kunisato
authors.forename: Takashi
authors.initials: T
authors.lastname: Asai
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Shimizu
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Go
authors.initials: G
authors.lastname: Yamamoto
authors.forename: Sachiko
authors.initials: S
authors.lastname: Seo
authors.forename: Keiki
authors.initials: K
authors.lastname: Kumano
authors.forename: Etsuko
authors.initials: E
authors.lastname: Nakagami-Yamaguchi
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Hamada
authors.forename: Shinichi
authors.initials: S
authors.lastname: Aizawa
authors.forename: Hisamaru
authors.initials: H
authors.lastname: Hirai
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D002352:Carrier Proteins; D002454:Cell Differentiation / Q000235:genetics* / Q000276:immunology; D019070:Cell Lineage / Q000235:genetics* / Q000276:immunology; D006579:Heterozygote; D051379:Mice; D018345:Mice, Knockout; D011506:Proteins / Q000378:metabolism; D051883:Receptor, Notch2; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D002352:Carrier Proteins; C497765:Notch2 protein, mouse; D011506:Proteins; D051883:Receptor, Notch2; D011956:Receptors, Cell Surface; C409218:deltex protein, vertebrate