Dentin-induced in vivo inflammatory response and in vitro activation of murine macrophages.
The activation of inflammatory cells and consequent release of mediators play an important role in the resorption of mineralized tissues. In the present study, we examined the ability of dentin extracts to induce inflammatory cell recruitment and activation. We showed here that dentin extracts triggered an intense cell migration and progressive cell maturation, in a time- and dose-dependent manner. Expression of interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), nitric oxide (NO), and hydrogen peroxide (H(2)O(2)) was also up-regulated by dentin extracts. These results show that inflammatory events can be elicited in response to dentin, which may suggest a possible involvement of dentin molecules in the inflammatory events, coupled with their release at the root resorption sites.
Lara
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VS
Department of Stomatology, Pathology, Bauru Dental School, University of São Paulo-Bauru, Rua Sérvio Túlio Carrijo Coube, 3-33, Apto 91-C, Jardim Infante Dom Henrique, 17012-632-Bauru, São Paulo, Brazil. vanessa@fob.usp.br
Figueiredo
F
F
da Silva
T A
TA
Cunha
F Q
FQ
D000704:Analysis of Variance; D000818:Animals; D018929:Cell Culture Techniques; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003804:Dentin / Q000502:physiology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis; D018836:Inflammation Mediators / Q000494:pharmacology*; D007375:Interleukin-1 / Q000032:analysis; D007962:Leukocytes / Q000187:drug effects; D008262:Macrophage Activation / Q000187:drug effects*; D017737:Macrophages, Peritoneal / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000032:analysis; D016877:Oxidants / Q000032:analysis; D013997:Time Factors; D014020:Tissue Extracts / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis; D015854:Up-Regulation
D018836:Inflammation Mediators; D007375:Interleukin-1; D016877:Oxidants; D014020:Tissue Extracts; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D006861:Hydrogen Peroxide