Generation of live fry from intraperitoneally transplanted primordial germ cells in rainbow trout.
Germ cell transplantation has tremendous applications in transgenic animal production, assisted reproductive technology, and germline stem cell research. Here, we report for the first time the production of individuals from intraperitoneally transplanted primordial germ cells (PGCs) in animals. To trace the behavior of exogenous PGCs in recipients, PGCs visualized by a green fluorescent protein gene were used as donors. The PGCs prepared from the genital ridges of hatching embryos were transplanted into recipients at various developmental stages. The PGCs injected into the peritoneal cavities of hatching embryos had the ability to migrate toward, and to colonize, the genital ridges of recipient embryos. Furthermore, donor-derived PGCs proliferated and differentiated into mature eggs and sperm in the allogenic gonads; the resulting gametes produced live fry, showing the donor-derived phenotype, through fertilization. Combined with in vitro culture, genetic modification, and cryopreservation of PGCs, this technique provides new approaches for fish bioengineering.
Takeuchi
Yutaka
Y
Department of Aquatic Biosciences, Tokyo University of Fisheries, Minato-ku, Tokyo 108-8477, Japan.
Yoshizaki
Goro
G
Takeuchi
Toshio
T
D000367:Age Factors; D000818:Animals; D017690:Cell Transplantation / Q000379:methods*; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000637:transplantation; D005260:Female; D005835:Genitalia / Q000196:embryology*; D005854:Germ Cells / Q000637:transplantation*; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D017686:Oncorhynchus mykiss / Q000196:embryology*; D010529:Peritoneal Cavity / Q000196:embryology; D010858:Pigmentation; D012099:Reproductive Techniques; D013094:Spermatozoa / Q000502:physiology
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins