[HPLC determination of two flavonoid compounds in Psoralea corylifolia].
OBJECTIVE
To determine two flavonoid compounds in Psoralea corylifolia L. (PC) simultaneously with HPLC method.


METHODS
Bavachin and corylin isolated from PC and purified in our laboratory were used as the reference compounds. The HPLC separation was carried out on an Techsphere ODS column using mobile phase consisting of a mixture of methanol and 20 mmol.L-1 ammonium acetate buffer pH 4.0 (67:33), and the UV detection wavelength was 240 nm.


RESULTS
Simultaneous determination of bavachin and corylin was achieved. The linear range was 1.25-20 micrograms.mL-1 for both bavachin and corylin. The average recovery of bavachin and corylin was 94.9% and 96.2%, and RSD was 3.1% and 3.6% respectively.


CONCLUSIONS
This is the first report on simultaneous determination of bavachin and corylin in PC with satisfactory accuracy and reproducibility.
Lin
Rui-min
RM
Department of Chemistry, Anshan Teacher's College, Anshan 114005, Liaoning, China.
Wang
Da-wei
DW
Xiong
Zhi-li
ZL
Xu
Yong
Y
Li
Fa-mei
FM
D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis*; D005638:Fruit / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D029907:Psoralea / Q000737:chemistry*
D005419:Flavonoids; C459212:bavachin; C459211:corylin