title: The role of organic solvents in the separation of nonionic compounds by capillary electrophoresis.
abstract: Although nonionic compounds can be separated by micellar electrokinetic chromatography (MEKC), application of this technique is restricted by a somewhat limited elution range. Incorporation of organic solvents in the background electrolyte (BGE) greatly extends the scope of MEKC and provides a major variable in optimizing the separation of neutral analytes. This paper provides a systematic review of the principles and scope of the separation of neutral analytes by capillary electrophoresis (CE) in organic-aqueous solution. The methods surveyed include those that use tetraalkylammonium salts, dioctyl sulfosuccinate, lauryl poly(oxyethylene) sulfate. Polyaromatic hydrocarbon (PAH) compounds can be separated using sodium hexadecyl sulfate in 70% methanol (30% aqueous) to 100% methanol.
authors.affiliation: Department of Chemistry, Iowa State University, Ames, IA 50011, USA. kniss@ameslab.gov
authors.forename: James S
authors.initials: JS
authors.lastname: Fritz
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D004143:Dioctyl Sulfosuccinic Acid / Q000302:isolation & purification*; D011092:Polyethylene Glycols / Q000302:isolation & purification*; D000644:Quaternary Ammonium Compounds / Q000302:isolation & purification*; D012967:Sodium Dodecyl Sulfate / Q000302:isolation & purification*; D012997:Solvents / Q000737:chemistry*
chemical_list: D000644:Quaternary Ammonium Compounds; D012997:Solvents; C482380:lauryl poly(oxyethylene)sulfate; D004143:Dioctyl Sulfosuccinic Acid; D012967:Sodium Dodecyl Sulfate; D011092:Polyethylene Glycols