title: Cellular immune responses in chickens induced by recombinant R7 Leucocytozoon caulleryi vaccine.
abstract: We have recently developed recombinant subunit vaccine consisting of second-generation schizont (2GS) membrane protein (rR7) of Leucocytozoon caulleryi. Chickens immunized with rR7 antigen acquired clear resistance to challenge by Leucocytozoon sporozoites. We examined the induction of cellular immune responses in vaccinated chickens. Spleen adherent cells from vaccinated chickens showed significantly higher phagocytic activity against 2GS-coated latex particles than did cells from adjuvant-inoculated or untreated control birds. Anti-R7 chicken IgG significantly increased the phagocytic rate of adherent cells from these 3 groups. These results show that specific cellular immune responses are induced by recombinant R7 subunit vaccine consisting of L. caulleryi 2GS protein, which suppresses the growth of parasites in the host in association with antiparasite antibodies to 2GS antigen.
authors.affiliation: Laboratory of Veterinary Immunology, Division of Veterinary Sciences, Graduate School of Agriculture and Biological Science, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan.
authors.forename: Kaoru
authors.initials: K
authors.lastname: Nakata
authors.forename: Shinobu
authors.initials: S
authors.lastname: Watarai
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kodama
authors.forename: Tohru
authors.initials: T
authors.lastname: Gotanda
authors.forename: Akira
authors.initials: A
authors.lastname: Ito
authors.forename: Katsumi
authors.initials: K
authors.lastname: Kume
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood / Q000276:immunology; D002448:Cell Adhesion; D002645:Chickens / Q000276:immunology* / Q000469:parasitology; D016787:Haemosporida / Q000276:immunology*; D007111:Immunity, Cellular; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology / Q000302:isolation & purification; D008264:Macrophages / Q000276:immunology; D010587:Phagocytosis; D011201:Poultry Diseases / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control*; D011529:Protozoan Infections, Animal / Q000276:immunology / Q000517:prevention & control*; D016052:Protozoan Vaccines / Q000276:immunology*; D011817:Rabbits; D013154:Spleen / Q000166:cytology / Q000276:immunology; D022223:Vaccines, Subunit / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D007074:Immunoglobulin G; D016052:Protozoan Vaccines; D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic