Extremely low frequency magnetic fields affect transposition activity in Escherichia coli.
The aim of this study was to verify whether extremely low frequency (ELF) magnetic fields (MF) could affect transposition activity like some environmental stress factors such as heat shock or UV irradiation. Using an Escherichia coli Lac Z(-) strain transformed with a plasmid containing a Tn 10 derivative element expressing beta-galactosidase only after transposition, it was possible to determine the events of transposition evaluating the rate at which the colonies developed dark coloured papillae (Lac Z(+)). We found that those bacteria that had been exposed for a long time (58 h) to a 50 Hz low intensity MF (0.1-1 mT) gave colonies with significantly lower transposition activity compared to sham-exposed bacteria. Such reduction in transposition activity was positively correlated to the intensity of the MF, in a dose-effect manner. This phenomenon was not affected by bacterial cell proliferation, since no significant differences were observed in number, diameter and perimeter between sham-exposed and MF-exposed colonies.
Del Re
B
B
Department of Evolutionary Experimental Biology, University of Bologna, Via Selmi 3, 40126 Bologna, Italy.
Garoia
F
F
Mesirca
P
P
Agostini
C
C
Bersani
F
F
Giorgi
G
G
D002453:Cell Cycle / Q000528:radiation effects; D015169:Colony Count, Microbial; D003116:Color; D004251:DNA Transposable Elements / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D004574:Electromagnetic Fields; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D007544:Isopropyl Thiogalactoside; D007763:Lac Operon; D010957:Plasmids; D012984:Software; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
D004251:DNA Transposable Elements; D007544:Isopropyl Thiogalactoside; D001616:beta-Galactosidase