Ion physiology of vitellogenic follicles.
The ion physiology of vitellogenic follicles from a lepidopteran (Hyalophora cecropia) and a hemipteran (Rhodnius prolixus) are compared. Similarities that can be expected to occur in vitellogenic follicles of many other insects include: (1) gap junctions, which unite the cells of a follicle into an integrated electrical system, (2) transmembrane K(+) and H(+) gradients that account for over 60% of follicular membrane potentials, (3) absence of a Cl(-) potential, (but the opening of channels to this anion when vitellogenesis terminates in H. cecropia), (4) an electrogenic proton pump that supplements follicular membrane potentials, (5) Ca(2+) action potentials evoked by injecting depolarizing currents into oocytes, and (6) the use of osmotic pressure to control epithelial patency. Differences include: a Na(+)/K(+)-ATPase that accounts for about 20% of the follicular resting potential in R. prolixus but is absent from H. cecropia, and an intrafollicular Ca(2+) current that moves from oocyte to nurse cells through cytoplasmic bridges in H. cecropia. Evidence is also summarized for two promising mechanisms that require further substantiation: (1) transmission via gap junctions of a follicle cell product that promotes endocytosis in the oocyte; and (2) transport of the proton pump back and forth between cell surface and endosomes as the membrane that carries it recycles through successive rounds of vitellogenin uptake.
Telfer
William H.
WH
Department of Biology, University of Pennsylvania, 19104-6018, Philadelphia, PA, USA
Woodruff
Richard I.
RI