[Proteomics-based identification of Maspin differential expression in bronchial epithelial immortalized cells and malignant transformation cells].
OBJECTIVE
Maspin, a serepin inhibitor, plays a key role in tumor growth and metastasis. The aim of this study was to identify the differential expression of Maspin in malignant transformation process of bronchial epithelial cells by proteomics.


METHODS
Functional proteomics analysis of Maspin on bronchial epithelial immortalized cells and malignant transformation cells was carried out using immobilized pH gradient (IPG) two-dimensional electrophoresis, peptide mass fingerprinting (PMF), and post source decay (PSD) of bio-mass spectrometry.


RESULTS
Nearly 1500 expressed proteins profile on bronchial epithelial immortalized cells and malignant transformation cells were obtained in the range of MW 14.4-94 kDa, PI 3-10. Image analysis showed that Maspin was down-regulated in malignant transformation cells compared with that in immortalized cells. Northern blot analysis showed that the mRNA abundance of Maspin in malignant transformation cells was much lower than that in immortalized cells.


CONCLUSIONS
Alteration expression of Maspin at transcription and translation levels might be involved in carcinogenesis of lung.
Xie
Ling
L
Institute of Radiation Medicine, Academy of Military Medicine, Beijing, PR China.
Ying
Wan-Tao
WT
Li
Bang-Yin
BY
Zhang
Kai-Tai
KT
Qian
Xiao-Hong
XH
Xiang
Xiao-Qiong
XQ
Wu
De-Chang
DC
D015152:Blotting, Northern; D001980:Bronchi / Q000166:cytology; D002461:Cell Line, Transformed / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D004847:Epithelial Cells / Q000378:metabolism*; D020869:Gene Expression Profiling; D016147:Genes, Tumor Suppressor; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D015843:Serpins / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D011506:Proteins; C085344:SERPIN-B5; D015843:Serpins