[The overexpression of prion protein in drug resistant gastric cancer cell line SGC7901/ADR and its significance].
OBJECTIVE
To investigate the overexpression of prion protein (PrP) in drug-resistant gastric cancer cell line SGC7901/ADR and its role in multidrug resistance in gastric cancer.


METHODS
(1) The expression of PrP in SGC7901/ADR, SGC790/VCR and their parental cell line SGC7901 was detected with Northern and Western blot at the mRNA and protein level. (2) Eukaryotic sense and antisense expression vector were constructed based on DNA recombination technology and (3) introduced into SGC7901 and SGC7901/ADR cell lines through electroporation. (4) The accumulation and retention of ADR in transiently transfected cells were detected by flow cytometry.


RESULTS
(1) Northern and western blot suggested significantly higher expression of PrP in SGC7901/ADR and SGC7901/VCR than that in SGC7901. (2) 48 hours after the vectors transfection, the average fluorescence intensity of Adr in transfected cells were detected. The accumulation intensity were 8.9 +/- 0.7 in BS, 6.6 +/- 0.3 in PS and 7.5 +/- 0.6 in PA. The retention intensity were 9.3 +/- 0.6 in SGC7901, 5.9 +/- 0.5 in PS and 7.1 +/- 0.5 in PA. There were significant difference between PS and BS with P < 0.01, as well as RA and BA with P < 0.01. These data suggested that PrP gene could affect the drug accumulation in gastric cancer cells after its transfected into cells.


CONCLUSIONS
PrP was highly expressed in gastric cancer cell lines SGC7901/ADR and SGC7901/VCR. Overexpression of PrP had certain effect on drug accumulation in gastric cancer cells.
Du
Jing-ping
JP
Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi'an 710033, Shaanxi Province, China.
Jin
Xiao-hang
XH
Shi
Yong-quan
YQ
Zhao
Yan-qiu
YQ
Liu
Chang-jiang
CJ
Cao
Yun-xin
YX
Qiao
Tai-dong
TD
Chen
Bao-jun
BJ
Fan
Dai-ming
DM
D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm; D006801:Humans; D011328:Prions / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D014162:Transfection
D011328:Prions; D012333:RNA, Messenger