Quantification of tamoxifen and three metabolites in plasma by high-performance liquid chromatography with fluorescence detection: application to a clinical trial.
A sensitive and reproducible assay employing liquid-liquid extraction and high-performance liquid chromatography with fluorescence detection for the quantification of tamoxifen, N-desmethyltamoxifen, 4-hydroxytamoxifen, and Z-4-hydroxy-N-desmethyltamoxifen in human plasma is described. The compounds and internal standard, propranolol, were separated with a cyano column and a mobile phase of acetonitrile-20 mM potassium phosphate buffer (pH 3; 35:65, v/v) then detected with fluorescence using a modified version of a method originally described by Fried and Wainer [J. Chromatogr. B 655 (1994) 261]. The coefficients of variation for the midpoint of the standard curve for each compound were less than 10%. This method was applied to a pharmacokinetic study of tamoxifen disposition in breast cancer patients.
Lee
Kyung-Hoon
KH
Division of Clinical Pharmacology, Indiana University School of Medicine, Wishard Memorial Hospital, 1001 West Tenth Street, Myers Building W7123, Indianapolis, IN 46202, USA.
Ward
Bryan A
BA
Desta
Zeruesenay
Z
Flockhart
David A
DA
Jones
David R
DR
D018931:Antineoplastic Agents, Hormonal / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002986:Clinical Trials as Topic; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013629:Tamoxifen / Q000097:blood* / Q000493:pharmacokinetics
D018931:Antineoplastic Agents, Hormonal; D013629:Tamoxifen