title: Aerobic denitrifying bacteria that produce low levels of nitrous oxide.
abstract: Most denitrifiers produce nitrous oxide (N(2)O) instead of dinitrogen (N(2)) under aerobic conditions. We isolated and characterized novel aerobic denitrifiers that produce low levels of N(2)O under aerobic conditions. We monitored the denitrification activities of two of the isolates, strains TR2 and K50, in batch and continuous cultures. Both strains reduced nitrate (NO(3)(-)) to N(2) at rates of 0.9 and 0.03 micro mol min(-1) unit of optical density at 540 nm(-1) at dissolved oxygen (O(2)) (DO) concentrations of 39 and 38 micro mol liter(-1), respectively. At the same DO level, the typical denitrifier Pseudomonas stutzeri and the previously described aerobic denitrifier Paracoccus denitrificans did not produce N(2) but evolved more than 10-fold more N(2)O than strains TR2 and K50 evolved. The isolates denitrified NO(3)(-) with concomitant consumption of O(2). These results indicated that strains TR2 and K50 are aerobic denitrifiers. These two isolates were taxonomically placed in the beta subclass of the class Proteobacteria and were identified as P. stutzeri TR2 and Pseudomonas sp. strain K50. These strains should be useful for future investigations of the mechanisms of denitrifying bacteria that regulate N(2)O emission, the single-stage process for nitrogen removal, and microbial N(2)O emission into the ecosystem.
authors.affiliation: Institute of Applied Biochemistry, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Takaya
authors.forename: Maria Antonina B
authors.initials: MA
authors.lastname: Catalan-Sakairi
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Sakaguchi
authors.forename: Isao
authors.initials: I
authors.lastname: Kato
authors.forename: Zhemin
authors.initials: Z
authors.lastname: Zhou
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Shoun
mesh_terms: D000332:Aerobiosis; D003470:Culture Media; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009566:Nitrates / Q000378:metabolism*; D009609:Nitrous Oxide / Q000378:metabolism*; D011549:Pseudomonas / Q000145:classification* / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014865:Waste Disposal, Fluid; D014871:Water Microbiology
chemical_list: D003470:Culture Media; D004275:DNA, Ribosomal; D009566:Nitrates; D012336:RNA, Ribosomal, 16S; D009609:Nitrous Oxide