[Hematopoietic reconstitution in early period after autologous and allogeneic peripheral blood stem cell transplantation].
OBJECTIVE
To investigate the ratio and amount of CD34+ cells and their subsets in mobilized peripheral blood (MPB) and leukapheresis products in patients receiving autologous peripheral blood stem cell transplantation (auto-PBSCT) and in allo-PBSCT donors, and clarify the role of CD34+ cells in post-transplantation hematopoietic reconstitution.


METHODS
Sixteen patients receiving auto-PBSCT and 24 allo-PBSCT donors were studied for CD34+ and CD34+CD38- cell ratios in the 115 MPB and their leukapheresis product samples by way of flow cytometry, and the time of hematopoietic recovery was observed in the two groups.


RESULTS
The CD34+ cell ratio in the MPB samples and leukapheresis products was significantly lower in the donors than in auto-PBSCT patients (P<0.05), but CD34+CD38- cell ratio showed no obvious difference between the two groups. The amount of mononuclear cells (MNCs) received by allo-PBSCT patients was much more than that received by auto-PBSCT patients (P<0.01). Similar average times for post-transplant neutrophils recovery to over 0.5x10(9)/L and platelets to over 2 x 10(10)/L (P>0.05) were observed between the groups, and in both of them, the recovery was related to the amount of MNCs, CD34+ and CD34+/CD38- cell infusion.


CONCLUSIONS
Irrespective of auto-PBSCT or allo-PBSCT, the number of CD34+ and CD34+CD38-cells infused after the transplantation is the key factor to influence the course of hemopoietic reconstitution.
Wei
Yong-qiang
YQ
Department of Hematology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. wyq@fimmu.com
Feng
Ru
R
Yi
Zheng-shan
ZS
Yang
Yi
Y
Liu
Qi-fa
QF
Sun
Jing
J
Meng
Fan-yi
FY
Zhou
Shu-yun
SY
D036541:ADP-ribosyl Cyclase / Q000032:analysis; D051997:ADP-ribosyl Cyclase 1; D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34 / Q000032:analysis; D002648:Child; D005260:Female; D006410:Hematopoiesis; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins; D008875:Middle Aged; D036102:Peripheral Blood Stem Cell Transplantation; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous
D015703:Antigens, CD; D018952:Antigens, CD34; D008562:Membrane Glycoproteins; D036541:ADP-ribosyl Cyclase; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1