title: Evaluation of the epidemiologic utility of secondary typing methods for differentiation of Mycobacterium tuberculosis isolates.
abstract: Spoligotyping and mycobacterial interspersed repetitive unit-variable-number tandem repeat analysis (MIRU-VNTR) were evaluated for the ability to differentiate 64 Mycobacterium tuberculosis isolates from 10 IS6110-defined clusters. MIRU-VNTR performed slightly better than spoligotyping in reducing the number of clustered isolates and the sizes of the clusters. All epidemiologically related isolates remained clustered by MIRU-VNTR but not by spoligotyping.
authors.affiliation: Department of Tropical Medicine, Tulane University School of Public Health and Tropical Medicine, New Orleans, Louisiana 70112, USA. Awewura_Kwara@brown.edu
authors.forename: Awewura
authors.initials: A
authors.lastname: Kwara
authors.forename: Ronald
authors.initials: R
authors.lastname: Schiro
authors.forename: Lauren S
authors.initials: LS
authors.lastname: Cowan
authors.forename: Newton E
authors.initials: NE
authors.lastname: Hyslop
authors.forename: Mark F
authors.initials: MF
authors.lastname: Wiser
authors.forename: Stephanie
authors.initials: S
authors.lastname: Roahen Harrison
authors.forename: Patricia
authors.initials: P
authors.lastname: Kissinger
authors.forename: Lois
authors.initials: L
authors.lastname: Diem
authors.forename: Jack T
authors.initials: JT
authors.lastname: Crawford
mesh_terms: D015373:Bacterial Typing Techniques; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D005838:Genotype; D006801:Humans; D018598:Minisatellite Repeats / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D009838:Oligodeoxyribonucleotides / Q000032:analysis; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D014376:Tuberculosis / Q000453:epidemiology* / Q000382:microbiology
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D009838:Oligodeoxyribonucleotides