title: Application of short monolithic columns for improved detection of viruses.
abstract: Monolithic chromatography media represent a novel generation of stationary phases introduced in the last 10-15 years providing a chromatography matrix with enhanced mass transfer and hydrodynamic properties. These features allow for an efficient and fast separation of especially large biomolecules like e.g., DNA and viruses. In this study, the enrichment of virus RNA on short monolithic columns prior to molecular detection of viruses is described. Measles and mumps viruses were chosen as model viruses. The results show that it is possible to bind viral RNA on monoliths and concentrate viral nucleic acids from a fairly dilute sample. Consequently, a potential application of short monolithic columns is the concentration of virus RNA to improve the sensitivity and selectivity of viral detection with the possibility of isolating viral RNA from cell-free biological fluids.
authors.affiliation: Institute of Immunology, Rockefeller Street 2, 10000, Zagreb, Croatia. kbranovic@imz.hr
authors.forename: Karmen
authors.initials: K
authors.lastname: Branovic
authors.forename: Dubravko
authors.initials: D
authors.lastname: Forcic
authors.forename: Jelena
authors.initials: J
authors.lastname: Ivancic
authors.forename: Ales
authors.initials: A
authors.lastname: Strancar
authors.forename: Milos
authors.initials: M
authors.lastname: Barut
authors.forename: Tanja
authors.initials: T
authors.lastname: Kosutic-Gulija
authors.forename: Renata
authors.initials: R
authors.lastname: Zgorelec
authors.forename: Renata
authors.initials: R
authors.lastname: Mazuran
mesh_terms: D002848:Chromatography, DEAE-Cellulose / Q000295:instrumentation / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008458:Measles Vaccine; D008459:Measles virus / Q000235:genetics / Q000302:isolation & purification*; D009108:Mumps Vaccine; D009109:Mumps virus / Q000235:genetics / Q000302:isolation & purification*; D012367:RNA, Viral / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014613:Vaccines, Attenuated
chemical_list: D008458:Measles Vaccine; D009108:Mumps Vaccine; D012367:RNA, Viral; D014613:Vaccines, Attenuated