Efficient production of Arthromyces ramosus peroxidase by Aspergillus awamori.
The heterologous production of Arthromyces ramosus peroxidase (ARP) was analysed in the filamentous fungus Aspergillus awamori under control of the inducible endoxylanase promoter. Secretion of active ARP was achieved up to 800 mg l(-1) in shake flask cultures. Western blot analysis showed that an rARP product of the correct molecular weight was produced. In contrast to several other studies about heterologous production of heme containing peroxidases, our results suggest that in A. awamori no heme limitation exists during overproduction of ARP.
Lokman
B Christien
BC
TNO Nutrition and Food Research, Department of Applied Microbiology and Gene Technology, P.O. Box 360, 3700 AJ, Zeist, The Netherlands. lokman@voeding.tno.nl
Joosten
Vivi
V
Hovenkamp
Jacqueline
J
Gouka
Robin J
RJ
Verrips
C Theo
CT
van den Hondel
Cees A M J J
CA
D001230:Aspergillus / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D001709:Biotechnology / Q000379:methods*; D015152:Blotting, Northern; D015139:Blotting, Southern; D005285:Fermentation; D006418:Heme / Q000378:metabolism; D003904:Mitosporic Fungi / Q000201:enzymology* / Q000235:genetics; D009195:Peroxidase / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D011401:Promoter Regions, Genetic
D006418:Heme; D009195:Peroxidase