Structure of the O-polysaccharide of Proteus vulgaris O44: a new O-antigen that contains an amide of D-glucuronic acid with L-alanine.
The O-polysaccharide of Proteus vulgaris O44, strain PrK 67/57 was studied by 1H and 13C NMR spectroscopy, including 2D COSY, TOCSY, ROESY, H-detected 1H, 13C HMQC, HMQC-TOCSY and HMBC experiments. The polysaccharide was found to contain an amide of D-glucuronic acid with L-alanine [D-GlcA6(L-Ala)], and the following structure of the linear pentasaccharide repeating unit was established: [structure: see text]. The structural data of the O-polysaccharide and the results of serological studies with P. vulgaris O44 O-antiserum showed that the strain studied is unique among Proteus bacteria, which is in agreement with its classification in a separate Proteus serogroup, O44.
Toukach
Filip V
FV
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russian Federation.
Perepelov
Andrei V
AV
Bartodziejska
Beata
B
Shashkov
Alexander S
AS
Blaszczyk
Aleksandra
A
Arbatsky
Nikolay P
NP
Rozalski
Antoni
A
Knirel
Yuriy A
YA
D000116:Acetylgalactosamine / Q000032:analysis; D000409:Alanine / Q000032:analysis; D000577:Amides / Q000737:chemistry; D000606:Amino Sugars / Q000032:analysis; D002240:Carbohydrate Sequence; D003429:Cross Reactions / Q000276:immunology; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D020723:Glucuronic Acid / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D019081:O Antigens / Q000737:chemistry* / Q000276:immunology; D011513:Proteus mirabilis / Q000737:chemistry / Q000276:immunology; D011514:Proteus vulgaris / Q000737:chemistry*
C108162:3-amino-3,6-dideoxygalactose; D000577:Amides; D000606:Amino Sugars; D019081:O Antigens; D020723:Glucuronic Acid; D005947:Glucose; D000116:Acetylgalactosamine; D000409:Alanine; D005690:Galactose