Rats and mice exhibit distinct inflammatory reactions after spinal cord injury.
Spinal contusion pathology in rats and mice is distinct. Cystic cavities form at the impact site in rats while a dense connective tissue matrix occupies the injury site in mice. Because inflammatory cells coordinate mechanisms of tissue injury and repair, we evaluated whether the unique anatomical presentation in spinally injured rats and mice is associated with a species-specific inflammatory response. Immunohistochemistry was used to compare the leukocytic infiltrate between rats and mice. Microglia/macrophage reactions were similar between species; however, the onset and magnitude of lymphocyte and dendritic cell (DC) infiltration were markedly different. In rats, T-cell numbers were highest between 3 and 7 days postinjury and declined by 50% over the next 3 weeks. In mice, significant T-cell entry was not evident until 14 days postinjury, with T-cell numbers doubling between 2 and 6 weeks. Dendritic cell influx paralleled T-cell infiltration in rats but was absent in mouse spinal cord. De novo expression of major histocompatability class II molecules was increased in both species but to a greater extent in mice. Unique to mice were cells that resembled lymphocytes but did not express lymphocyte-specific markers. These cells extended from blood vessels within the fibrotic tissue matrix and expressed fibronectin, collagen I, CD11b, CD34, CD13, and CD45. This phenotype is characteristic of fibrocytes, specialized blood-borne cells involved in wound healing and immunity. Thus, species-specific neuroinflammation may contribute to the formation of distinct tissue environments at the site of spinal cord injury in mice and rats.
Sroga
Julie M
JM
Department of Molecular Virology, Immunology and Medical Genetics, The Ohio State University College of Medicine and Public Health, Columbus, Ohio 43210, USA.
Jones
T Bucky
TB
Kigerl
Kristina A
KA
McGaughy
Violeta M
VM
Popovich
Phillip G
PG
D000818:Animals; D016516:CD4-CD8 Ratio; D003713:Dendritic Cells / Q000276:immunology / Q000473:pathology; D005260:Female; D005355:Fibrosis; D008264:Macrophages / Q000276:immunology / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL / Q000276:immunology*; D017628:Microglia / Q000276:immunology / Q000473:pathology; D009187:Myelitis / Q000276:immunology* / Q000473:pathology; D051381:Rats; D011917:Rats, Inbred Lew / Q000276:immunology*; D013045:Species Specificity; D013119:Spinal Cord Injuries / Q000276:immunology* / Q000473:pathology; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000473:pathology; D014945:Wound Healing / Q000276:immunology