Integrated capillary fluorescence DNA biosensor.
Covalent attachment of dsDNA molecules inside a glass capillary without the need for hybridization is described. It is shown that the glass capillary has a surface density of 2.5 x 10(13) molecules/cm(2) with specific binding capacity of 62.5%. The resulting substrate was used to develop a biosensor for determining fluorescent organic analytes and metal binding with DNA. The biosensor combines highly specific immobilization chemistry with a capillary-geometry flow cell arrangement. The results show that fluorescent dyes are retained in the dsDNA-modified surface and that exposure to concentrations of nickel and lead ions resulted in a recoverable, highly reproducible diminishment of the fluorescence intensity.
Breimer
Marc A
MA
Department of Chemistry, State University of New York at Binghamton, PO Box 6016, Binghamton, NY 13902-6016, USA.
Gelfand
Yevgeny
Y
Sadik
Omowunmi A
OA
D000327:Adsorption; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002197:Capillary Action; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000032:analysis* / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D017022:Flow Injection Analysis / Q000295:instrumentation* / Q000379:methods; D016231:Fluorescence Polarization Immunoassay / Q000295:instrumentation* / Q000379:methods; D005456:Fluorescent Dyes; D007854:Lead / Q000032:analysis* / Q000737:chemistry; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods; D009532:Nickel / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods; D018511:Systems Integration
D004268:DNA-Binding Proteins; D005456:Fluorescent Dyes; D007854:Lead; D009532:Nickel; D004247:DNA