Cloning and sequence analysis of the nucleocapsid gene of porcine epidemic diarrhea virus Chinju99.
The nucleocapsid (N) gene of the porcine epidemic diarrhea virus (PEDV) Chinju99 which was previously isolated in Chinju, Korea was cloned and sequenced to establish the information for the development of genetically engineered diagnostic reagents. Also, sequences of the nucleotides and deduced amino acids of the Chinju99 N gene were analyzed by alignment with those of CV777 and Brl/87. The nucleotide sequence encoding the entire N gene open reading frame (ORF) of Chinju99 was 1326 bases long and encoded a protein of 441 amino acids with predicted M(r) of 49 kDa. It consisted of 405 adenine (30.5%), 293 cytosine (22.1%), 334 guanines (25.2%) and 294 thymines (22.2%) residues. The Chinju99 N ORF nucleotide sequence was 96.5% and 96.4% homologous with that of the CV777 and Brl/87, respectively. The Chinju99 N protein revealed 96.8% amino acid identity with that of Brl/87 and CV777, respectively. The amino acid sequence contained seven potential sites for threonine (T)- or serine (S)-linked phosphorylation by each protein kinase C and casein kinase II.
Lee
Hee-Kyung
HK
College of Veterinary Medicine, Institute of Animal Medicine, Gyeongsang National University, Chinju 660-701, Republic of Korea.
Yeo
Sang-Geon
SG
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017934:Coronavirus / Q000235:genetics* / Q000302:isolation & purification; D018352:Coronavirus Infections / Q000662:veterinary / Q000821:virology; D018076:DNA, Complementary / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D007723:Korea; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins / Q000235:genetics*; D016366:Open Reading Frames; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D034421:Sus scrofa; D013553:Swine Diseases / Q000821:virology
D018076:DNA, Complementary; D004279:DNA, Viral; D019590:Nucleocapsid Proteins