title: Xerocomus chrysenteron lectin: identification of a new pesticidal protein.
abstract: Xerocomus chrysenteron is an edible mushroom with insecticidal properties. In an earlier work, we found that proteins are responsible for this toxicity. Here we describe the purification of a approximately 15 kDa lectin, named XCL, from the mushroom. Its cDNA and gDNA were cloned by PCR strategies and a recombinant form was expressed in Escherichia coli. Sequence alignments and sugar specificity showed that this protein is the third member of a new saline-soluble lectin family present in fungi. This protein, either purified from mushroom or expressed in vitro in E. coli, was found to be toxic to some insects, such as the dipteran Drosophila melanogaster and the hemipteran, Acyrthosiphon pisum. The lectin possesses a high insecticidal activity compared to lectin isolated from leguminosae (Lathyrus ochrus) or from the snowdrop (Galanthus nivalis).
authors.affiliation: IPBS-UMR 5089, Groupe de Biophysique et de Biotechnologie des Protéines, 205 route de Narbonne, 31077 Toulouse Cedex 4, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Trigueros
authors.forename: Andrée
authors.initials: A
authors.lastname: Lougarre
authors.forename: Djamila
authors.initials: D
authors.lastname: Ali-Ahmed
authors.forename: Yvan
authors.initials: Y
authors.lastname: Rahbé
authors.forename: Jean
authors.initials: J
authors.lastname: Guillot
authors.forename: Louis
authors.initials: L
authors.lastname: Chavant
authors.forename: Didier
authors.initials: D
authors.lastname: Fournier
authors.forename: Laurent
authors.initials: L
authors.lastname: Paquereau
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001487:Basidiomycota / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D004331:Drosophila melanogaster; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000633:toxicity; D005800:Genes, Fungal; D007306:Insecticides; D037102:Lectins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000633:toxicity; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D018675:Toxicity Tests
chemical_list: D005656:Fungal Proteins; D007306:Insecticides; D037102:Lectins; D010455:Peptides