XANES determination of the platinum oxidation state distribution in cancer cells treated with platinum(IV) anticancer agents.
Here we describe the use of X-ray absorption near edge spectroscopy (XANES) to provide information about the relative proportions of platinum(II) and platinum(IV) complexes by analyzing the XANES edge height. The intracellular reduction of platinum(IV) complexes in cancer cells has been observed directly, and the proportion of reduction after 2 h was found to correlate with the reduction potentials of the complexes.
Hall
Matthew D
MD
Centre for Heavy Metals Research, School of Chemistry, University of Sydney, Sydney, NSW 2006, Australia.
Foran
Garry J
GJ
Zhang
Mei
M
Beale
Philip J
PJ
Hambley
Trevor W
TW
D000970:Antineoplastic Agents / Q000737:chemistry / Q000493:pharmacokinetics*; D005260:Female; D006801:Humans; D009944:Organoplatinum Compounds / Q000737:chemistry / Q000493:pharmacokinetics*; D010051:Ovarian Neoplasms / Q000737:chemistry / Q000188:drug therapy / Q000378:metabolism*; D010084:Oxidation-Reduction; D010984:Platinum / Q000737:chemistry / Q000493:pharmacokinetics*; D013052:Spectrometry, X-Ray Emission; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D009944:Organoplatinum Compounds; D010984:Platinum