[Changes of STAT3 in cell replicative senescence and effects of angiotensin II on STAT3].
OBJECTIVE
To investigate the changes of STAT3 in cell replicative senescence and the effects of angiotensin II (AngII) on STAT3.


METHODS
Normal human fetal lung diploid fibroblast cell line WI-38 was cultured and the senescent cell was defined with beta-gal staining. Electrophoretic mobility shift assay and Western blotting assay were used to detect the activation of STAT3 during cell replicative senescence and examine the process of STAT3 activation with AngII.


RESULTS
The synthesis of STAT3 protein during the course of cell replicative senescent increased, the nuclear translocation of phosphorylated STAT3 decreased, and the activities of STAT3 binding DNA reduced.


CONCLUSIONS
AngII induces the activation of STAT3 in both mature and senescence cell by AT(1) receptor, however, the reaction of senescent cell to AngII is weaker. The site of block may be in the nuclear translocation. AngII induces the activity of STAT3 in replicative senescence WI-38.
Wang
Xiao-dan
XD
Department of Nephrology, General Hospital of the People's Liberation Army, Beijing 100853, China.
Chen
Xiang-mei
XM
Zhou
Feng
F
Fu
Bo
B
Hong
Quan
Q
Feng
Zhe
Z
Wang
Jian-zhong
JZ
Fan
Dai-ming
DM
D021581:Active Transport, Cell Nucleus; D000804:Angiotensin II / Q000494:pharmacology*; D002460:Cell Line; D016922:Cellular Senescence / Q000502:physiology*; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D006801:Humans; D010766:Phosphorylation; D050796:STAT3 Transcription Factor; D015534:Trans-Activators / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism
D004268:DNA-Binding Proteins; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D015534:Trans-Activators; D000804:Angiotensin II; D004247:DNA; D001616:beta-Galactosidase