Effects of a vegetable extract from Lupinus albus (LU105) on the production of matrix metalloproteinases (MMP1, MMP2, MMP9) and tissue inhibitor of metalloproteinases (TIMP1, TIMP2) by human gingival fibroblasts in culture.
This study examined the effects of a vegetable extract from Lupinus albus (LU105) on MMPs and TIMPs secreted by human gingival fibroblasts in culture. LU105 was extracted from seeds of L. albus and is freely soluble in water. Gelatin zymography showed that control human gingival fibroblasts maintained in culture for 48 h express pro-MMP2 (progelatinase A) in the culture medium while the active form of MMP2 (gelatinase A), the active form of MMP9 (gelatinase B), and pro-MMP9 (progelatinase B) are not detected. Fibroblasts derived from inflamed gingiva expressed in the culture medium increased amounts of pro-MMP2 (progelatinase A) compared with controls and significant amounts of pro-MMP9 (progelatinase B). LU105 diminished the expression by gingival fibroblasts derived from inflamed tissue of both pro-MMP2 and pro-MMP9. Furthermore LU105 did not modify the amount of TIMP2 expressed in culture by controls or by gingival fibroblasts derived from inflamed tissue. TIMP1 and MMP1 significantly decreased when LU105 was added in the culture media of gingival fibroblasts derived from inflamed tissue compared with control fibroblasts. Thus LU105 seems to offer an opportunity to restore a correct balance between MMP2, MMP9, MMP1, and their natural inhibitors, i.e., TIMP1 and TIMP2 in human inflamed gingiva.
Gaultier
F
F
Laboratory of Physiopathology of Non-mineralised Tissues, U.F.R. Odontology, University René Descartes Paris V, 1 rue M. Arnoux, 92120, Montrouge, France.
Foucault-Bertaud
A
A
Lamy
E
E
Ejeil
A L
AL
Dridi
S M
SM
Piccardi
N
N
Piccirilli
A
A
Msika
P
P
Godeau
G
G
Gogly
B
B
D000293:Adolescent; D000328:Adult; D000704:Analysis of Variance; D015153:Blotting, Western; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology; D005881:Gingiva / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology*; D006801:Humans; D031297:Lupinus; D061965:Matrix Metalloproteinase Inhibitors; D020782:Matrix Metalloproteinases / Q000032:analysis / Q000096:biosynthesis / Q000187:drug effects*; D009842:Oligopeptides / Q000494:pharmacology*; D010518:Periodontitis / Q000201:enzymology*; D010936:Plant Extracts / Q000494:pharmacology*; D011480:Protease Inhibitors / Q000494:pharmacology*; D012639:Seeds; D019714:Tissue Inhibitor of Metalloproteinases / Q000032:analysis / Q000096:biosynthesis / Q000187:drug effects
C481500:Lupinus albus extract 105; D061965:Matrix Metalloproteinase Inhibitors; D009842:Oligopeptides; D010936:Plant Extracts; D011480:Protease Inhibitors; D019714:Tissue Inhibitor of Metalloproteinases; D020782:Matrix Metalloproteinases