title: Enhanced production of podophyllotoxin by Podophyllum hexandrum using in situ cell retention bioreactor.
abstract: The rhizomes of the rare plant Podophyllum hexandrum contain podophyllotoxin, which is a precursor of the anticancer drugs etoposide and teniposide. Batch cultivation of Podophyllum hexandrum was conducted using optimized medium in a 3 L bioreactor, which resulted in biomass and podophyllotoxin concentrations of 21.4 g/L and 13.8 mg/L in 24 and 26 days, respectively. The batch kinetics was used to identify the mathematical model. The model was extrapolated to identify the nutrient feeding rate (150 mL/d) and substrate concentration (105 g/L) in the incoming feed for nonlimiting and noninhibitory glucose concentration in the cell retention bioreactor. An improvement in cell growth to 53 g/L and intracellular podophyllotoxin accumulation of 48.8 mg/L was achieved in 60 days, when the bioreactor was operated in continuous cell retention cultivation mode.
authors.affiliation: Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India.
authors.forename: Saurabh
authors.initials: S
authors.lastname: Chattopadhyay
authors.forename: Virendra S
authors.initials: VS
authors.lastname: Bisaria
authors.forename: Ashok K
authors.initials: AK
authors.lastname: Srivastava
mesh_terms: D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002455:Cell Division; D002478:Cells, Cultured; D003198:Computer Simulation; D007700:Kinetics; D008954:Models, Biological; D011034:Podophyllotoxin / Q000096:biosynthesis*; D011035:Podophyllum / Q000145:classification / Q000254:growth & development* / Q000378:metabolism*; D011786:Quality Control
chemical_list: D011034:Podophyllotoxin