Determination of efficiency of attachment of biotinylated antibodies to avidin-linked, aqueous-based microcapsules by flow cytometry.
We used flow cytometry to determine the percentage of aqueous-based microcapsules bearing antibodies specific for various antigen-presenting cells (APCs) within a given population of putative APC-specific microcapsules. Flow cytometry offers a high-throughput, rapid and simple method to analyze antibody binding to noncellular, nonspherical material.
Chow
Andrea A
AA
University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
Moser
Charlotte A
CA
Speaker
Tully J
TJ
Offit
Paul A
PA
D000818:Animals; D000906:Antibodies / Q000378:metabolism*; D000938:Antigen-Presenting Cells / Q000378:metabolism*; D001360:Avidin / Q000378:metabolism*; D001710:Biotin / Q000378:metabolism; D002214:Capsules; D005434:Flow Cytometry / Q000379:methods*
D000906:Antibodies; D002214:Capsules; D001360:Avidin; D001710:Biotin