A simple and rapid fluorescence-based immunoassay for the detection of staphylococcal enterotoxin B.
The bioterrorism threat is perceived to be a real challenge to our nation's security. This threat has necessitated the design of better and faster assays for the detection of biothreat agents including staphylococcal enterotoxin B (SEB), a causative agent of food poisoning. This study describes a simple, fast and highly sensitive fluorescence-based immunoassay, in which the antibody is fluorescently-labeled for use in this assay. Use of labeled antibodies resulted in very low level of detection of SEB, 100 pg/well. This method is four times faster than classical and conventional enzyme-linked immunosorbent assay (ELISA).
Khan
Akbar S
AS
US Army Edgewood Chemical Biological Centre, AMSSB-RRT, E3330/143, 5183 Blackhawk Road, APG, MD 21010-5424, USA. akbar.khan@sbccom.apgea.army.mil
Cao
Cheng J
CJ
Thompson
Roy G
RG
Valdes
James J
JJ
D023482:Bioterrorism / Q000517:prevention & control; D004768:Enterotoxins / Q000032:analysis*; D015200:Fluoroimmunoassay / Q000379:methods* / Q000592:standards; D012680:Sensitivity and Specificity; D018089:Superantigens / Q000032:analysis; D013997:Time Factors
D004768:Enterotoxins; D018089:Superantigens; C031818:enterotoxin B, staphylococcal