The effects of mechanical strain on synovial fibroblasts.
OBJECTIVE
Arthritic diseases of the temporomandibular joint, such as rheumatoid arthritis and osteoarthritis, suggest that inflammatory mediators and metalloproteinases may play a role in their pathogenesis. Recent clinical evidence from physical therapy and other modalities has shown a significant decrease in temporomandibular joint symptoms in patients with early disease. This project examines the effect of mechanical strain on synovial fibroblasts' production of inflammatory mediators including prostaglandin E(2) (PGE(2)) and proteinases.


METHODS
An established synovial fibroblast cell line (HIG-82) was grown to confluency in modified Eagle's medium supplemented with 10% fetal calf serum. The monolayer of fibroblasts was then subjected to mechanical strain using the Flexercell Strain Unit (Flexcell International Corporation, McKeesport, PA) at 3 cycles per minute, with 10 seconds' elongation of up to 24% and 10 seconds of relaxation. Levels of PGE(2) were determined by radioimmunoassay using commercially available product and measured in nanograms per milliliter of supernatant. Proteinases collagenase, gelatinase, and stromelysin were measured by H(3) radioactive labeling of acidic anhydride to the specific substrate. Enzymatic proteolysis of the radiolabeled substrate was then measured in supernate as units per milliliter. Statistical analysis of all results was performed using Student's t test in triplicate.


RESULTS
PGE(2) levels of mechanically activated cells was 18.1 +/- 13.4 ng/mL, with control levels being 58.0 +/- 9.2 ng/mL. This is a statistically significant decrease, between strained and unstrained cells with P <.05. In control cells, proteinase activity that degrades collagen, gelatin, or casein was 4.27 +/- 1.5, 4.62 +/- 0.11, or 0.11 +/- 0.01 U/mL, respectively. Levels for mechanically strained cells were 3.99 +/- 1.90, 4.02 +/- 0.90, and 0.12 +/- 0.01 U/mL, respectively. These results show that there is a significant decrease in PGE(2) levels of synovial fibroblasts undergoing mechanical strain. Proteinases examined show no difference in levels between mechanically activated fibroblasts and their controls.


CONCLUSIONS
This decrease in PGE(2) production in synovial fibroblasts could help elucidate the mechanism by which physical therapy, and in particular continuous passive motion, may decrease inflammatory mediators of the temporomandibular joint.
Sambajon
Voltaire V
VV
Department of Oral and Maxillofacial Surgery, University of Pittsburgh, Pittsburgh, PA 15216, USA.
Cillo
Joseph E
JE
Gassner
Robert J
RJ
Buckley
Michael J
MJ
D000818:Animals; D001168:Arthritis / Q000201:enzymology / Q000628:therapy; D002460:Cell Line; D015232:Dinoprostone / Q000378:metabolism; D005109:Extracellular Matrix / Q000201:enzymology; D005347:Fibroblasts / Q000201:enzymology; D020782:Matrix Metalloproteinases / Q000378:metabolism; D015915:Motion Therapy, Continuous Passive; D011817:Rabbits; D013314:Stress, Mechanical; D013583:Synovial Membrane / Q000166:cytology / Q000201:enzymology*; D013704:Temporomandibular Joint / Q000201:enzymology; D013705:Temporomandibular Joint Disorders / Q000201:enzymology* / Q000628:therapy
D020782:Matrix Metalloproteinases; D015232:Dinoprostone