Improved simultaneous determination method of beta-carotene and retinol with saponification in human serum and rat liver.
Among the many simultaneous determination methods for carotenoid and retinoid, there are only a few reports including the saponification process. However, the yields of beta-carotene and retinol were higher when using this process. In this study, the analytical conditions, including saponification, were investigated. The extraction solvent was n-hexane and the sample solvent was HPLC mobile phase in the beta-carotene and retinol analysis. BHT as an antioxidant was added at concentrations of 0.125 and 0.025%, respectively, to ethanol and n-hexane phase in the extraction process for serum. The recovery rates were 99.7, 93.7 and 98.3% for beta-carotene, retinol and retinyl palmitate in serum, respectively, and 107.1, 92.8 and 98.8% for beta-carotene, retinol and retinyl palmitate in liver, respectively. The within-day coefficients of variation (C.V.) were 6.0% for serum and 4.7% for liver in the case of beta-carotene, 7.1% for serum, and 5.1% for liver in the case of retinol. The between-day coefficients of variation were 2.7% for serum and 2.7% for liver in the case of beta-carotene, and for retinol, 6.4% for serum and 2.7% for liver.
Hosotani
Keisuke
K
Faculty of Education, Wakayama University, 930 Sakaidani, Wakayama 640-8510, Japan. hosotani@center.wakayama-u.ac.jp
Kitagawa
Masahiro
M
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D006801:Humans; D008099:Liver / Q000378:metabolism*; D051381:Rats; D012015:Reference Standards; D015203:Reproducibility of Results; D014801:Vitamin A / Q000097:blood / Q000378:metabolism*; D019207:beta Carotene / Q000097:blood / Q000378:metabolism*
D019207:beta Carotene; D014801:Vitamin A