Application of short monolithic columns for improved detection of viruses.
Monolithic chromatography media represent a novel generation of stationary phases introduced in the last 10-15 years providing a chromatography matrix with enhanced mass transfer and hydrodynamic properties. These features allow for an efficient and fast separation of especially large biomolecules like e.g., DNA and viruses. In this study, the enrichment of virus RNA on short monolithic columns prior to molecular detection of viruses is described. Measles and mumps viruses were chosen as model viruses. The results show that it is possible to bind viral RNA on monoliths and concentrate viral nucleic acids from a fairly dilute sample. Consequently, a potential application of short monolithic columns is the concentration of virus RNA to improve the sensitivity and selectivity of viral detection with the possibility of isolating viral RNA from cell-free biological fluids.
Branovic
Karmen
K
Institute of Immunology, Rockefeller Street 2, 10000, Zagreb, Croatia. kbranovic@imz.hr
Forcic
Dubravko
D
Ivancic
Jelena
J
Strancar
Ales
A
Barut
Milos
M
Kosutic-Gulija
Tanja
T
Zgorelec
Renata
R
Mazuran
Renata
R
D002848:Chromatography, DEAE-Cellulose / Q000295:instrumentation / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008458:Measles Vaccine; D008459:Measles virus / Q000235:genetics / Q000302:isolation & purification*; D009108:Mumps Vaccine; D009109:Mumps virus / Q000235:genetics / Q000302:isolation & purification*; D012367:RNA, Viral / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014613:Vaccines, Attenuated
D008458:Measles Vaccine; D009108:Mumps Vaccine; D012367:RNA, Viral; D014613:Vaccines, Attenuated