title: The human solute carrier SLC41A1 belongs to a novel eukaryotic subfamily with homology to prokaryotic MgtE Mg2+ transporters.
abstract: We report here the first identification and structural characterization of a eukaryotic protein with homology to the bacterial MgtE family of potential Mg(2+) transporters. This human protein, denoted solute carrier family 41 member 1 (SLC41A1), consists of 513 amino acids with an estimated molecular weight of 56kDa. Computer analysis of the protein structure reveals that the protein consists of 10 putative transmembrane domains and includes two distinct domains highly homologous to the integral membrane part of the bacterial MgtE protein family. The gene encoding SLC41A1 is found on chromosome 1 (1q31-32) and the protein coding sequence is found on 10 exons. A 5-kb long transcript is identified in various human tissues with highest expression levels in heart and testis. We have also identified 10 SLC41A1 homologs in Homo sapiens, Mus musculus, Drosophila melanogaster, Anopheles gambiae, and Caenorhabditis elegans, and propose that these hypothetical proteins belong to a novel eukaryotic gene family.
authors.affiliation: Department of Immunology, The Norwegian Radium Hospital, Montebello, 0310, Oslo, Norway.
authors.forename: Troels
authors.initials: T
authors.lastname: Wabakken
authors.forename: Edith
authors.initials: E
authors.lastname: Rian
authors.forename: Marit
authors.initials: M
authors.lastname: Kveine
authors.forename: Hans-Christian
authors.initials: HC
authors.lastname: Aasheim
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017920:Antiporters / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D027682:Cation Transport Proteins; D002460:Cell Line; D002878:Chromosomes, Human, Pair 1; D018076:DNA, Complementary; D006801:Humans; D008274:Magnesium / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D014018:Tissue Distribution
chemical_list: D017920:Antiporters; D001426:Bacterial Proteins; D027682:Cation Transport Proteins; D018076:DNA, Complementary; D026901:Membrane Transport Proteins; C092192:MgtE protein, bacteria; D012333:RNA, Messenger; C476304:SLC41A1 protein, human; D008274:Magnesium