Fluorescence spectral imaging of dihydroxyacetone on skin in vivo.
Dihydroxyacetone (DHA) has been proposed as a potential alternative to dansyl chloride for use as a fluorescence marker on skin to assess stratum corneum turnover time in vivo. However, the fluorescence from DHA on skin has not been adequately studied. To address this void, a noninvasive, noncontact spectral imaging system is used to characterize the fluorescence spectrum of DHA on skin in vivo and to determine the optimal wavelengths over which to collect the DHA signal that minimizes the contributions from skin autofluorescence. The DHA-skin fluorescence signal dominates the 580-680 nm region of the visible spectrum when excited with ultraviolet radiation in the 320-400 nm wavelength region (UVA). An explanation of the time-dependent spectral features is proposed in terms of DHA polymerization and binding to skin.
Forest
Susan E
SE
Corporate Analytical, Corporate Research Division, The Procter & Gamble Company, Miami Valley Laboratories, Cincinnati, OH 45252, USA. forest.se@pg.com
Grothaus
Jeff T
JT
Ertel
Keith D
KD
Rader
Charlie
C
Plante
Janyl
J
D003952:Diagnostic Imaging; D004098:Dihydroxyacetone; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D012867:Skin / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D004098:Dihydroxyacetone