title: Influence of hepatitis B virus genotypes on the development of preS deletions and advanced liver disease.
abstract: Hepatitis B virus (HBV) mutants with deletions in the preS region have not been evaluated for association with viral genotypes. In a case-control study, HBV DNA samples collected from 80 each of carriers infected with HBV genotype B or C were examined for preS deletions. PreS deletion mutants were found in a total of 37 of 160 (23%) HBV carriers. Carriers with preS deletion mutants were older (56.0 +/- 12.7 vs 49.3 +/- 16.9 years, P < 0.05), were infected more frequently with HBV genotype C (84% vs 40%, P < 0.05), and had more advanced disease, such as liver cirrhosis and hepatocellular carcinoma (54% vs 31%; P < 0.05), than did those without such mutants. In a multivariate analysis, genotype C (odds ratio [OR] = 9.3, P < 0.001) and advanced liver disease (OR = 3.1, P < 0.01) were the most significant variables in association with preS deletions. A direct repeat sequence (TCAGG) was found at the start or at the end of preS1 deletions in 6 of the 20 (30%) cases examined, and preS2 deletions in these cases were clustered over the 5'-terminal half of this region. These results indicate that the development of preS deletion mutants depends on HBV genotypes and that it may be associated with progressive liver disease.
authors.affiliation: Department of Internal Medicine and Molecular Science, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.
authors.forename: Fuminaka
authors.initials: F
authors.lastname: Sugauchi
authors.forename: Tomoyoshi
authors.initials: T
authors.lastname: Ohno
authors.forename: Etsuro
authors.initials: E
authors.lastname: Orito
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sakugawa
authors.forename: Takafumi
authors.initials: T
authors.lastname: Ichida
authors.forename: Masafumi
authors.initials: M
authors.lastname: Komatsu
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Kuramitsu
authors.forename: Ryuzo
authors.initials: R
authors.lastname: Ueda
authors.forename: Yuzo
authors.initials: Y
authors.lastname: Miyakawa
authors.forename: Masashi
authors.initials: M
authors.lastname: Mizokami
mesh_terms: D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D006528:Carcinoma, Hepatocellular / Q000503:physiopathology / Q000821:virology; D016022:Case-Control Studies; D005260:Female; D017353:Gene Deletion; D005838:Genotype; D006514:Hepatitis B Surface Antigens / Q000235:genetics*; D006515:Hepatitis B virus / Q000145:classification* / Q000235:genetics*; D019694:Hepatitis B, Chronic / Q000503:physiopathology* / Q000821:virology; D006801:Humans; D008103:Liver Cirrhosis / Q000503:physiopathology / Q000821:virology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000235:genetics*
chemical_list: D006514:Hepatitis B Surface Antigens; D011498:Protein Precursors