[The role of the serotonin system in the stress response of various cells].
The recombinant mouse brain serotonin receptor (5HT1c) was used to study the response of plant cells and oocytes to a stress signal activated by the serotonin-serotonin receptor interaction and associated Ca2+ flow. Based on plant expression vectors, recombinant constructs were obtained to direct production of 5HT1c fused with the green fluorescent protein in plant cells. The mRNAs for hybrid proteins were synthesized in an in vitro transcription system. The expression and function of the hybrid protein and the function of the associated ion channels were electrophysiologically studied in Xenopus laevis oocytes injected with the hybrid mRNA. The hybrid protein was functional and changed the operation of the Ca2+ channel in oocytes. To study the expression of the hybrid constructs in plant cells, the in vitro transcription product was inoculated in tobacco leaves, which then fluoresced.
Belzhelarskaia
S N
SN
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia. belj@eimb.ru
Satton
F F
FF
D000818:Animals; D001921:Brain / Q000378:metabolism; D015220:Calcium Channels / Q000378:metabolism; D002478:Cells, Cultured; D004594:Electrophysiology / Q000379:methods; D005260:Female; D005786:Gene Expression Regulation; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D009865:Oocytes / Q000502:physiology; D010957:Plasmids / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D011985:Receptors, Serotonin / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012701:Serotonin / Q000502:physiology*; D015398:Signal Transduction; D013312:Stress, Physiological; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D014981:Xenopus
D015220:Calcium Channels; D008164:Luminescent Proteins; D012333:RNA, Messenger; D011985:Receptors, Serotonin; D011994:Recombinant Proteins; C112812:serotonin 1C receptor; D049452:Green Fluorescent Proteins; D012701:Serotonin