[Comparative studies with three different bovine blood sample BHV-1 ELISA tests: indirect ELISA and bG-blocking-ELISA].
A total of 2754 bovine blood samples were examined for BHV1 antibodies in three different BHV-1 ELISA tests. The tests used were the CHEKIT Trachitest 2nd. Gen. "Bestätigungstest" Serum, the CHEKIT-BHV-1-gB-ELISA (Bommeli-Diagnostics/Intervet), and the BHV-1-gB Antibody Test Kit (IDEXX). A first testing identified 111 (4.0%) of the samples as positive and 2501 (90.8%) samples negative in all three tests. Onehundredand-fortytwo (5.2%) of the samples showed a not negative result in at least one of the two gB-ELISAs. The testing was repeated for 139 of the 142 samples and still 89 (3.2%) samples were found with discrepant results. The remaining 50 samples showed a negative reaction in the three ELISA tests. After Western blot analysis antibodies against glycoprotein B (gB) of BHV-1 were only detected in 11 of the 89 samples with a not negative reaction in at least one of the gB-ELISAs. Three of the 50 ELISA-negative samples showed a positive reaction in the Western blot. The high number of positive results in the gB-ELISAs which were negative in the Western blot assay leads to the conclusion that gB-ELISAs may have a lower specificity as the indirect ELISA. Introducing the gB-ELISA as the standard test for BHV1 serology in Bavaria would result to an increase of the number of BHV-1 positive farms by 8% (gB IDEXX) or 20% (gB Bommeli) 20%.
Isa
Gisela
G
Zentralinstitut des Tiergesundheitsdienstes Bayern e. V., Staatliches Veterinäruntersuchungsamt Aulendorf.
Schelp
Christian
C
Truyen
Uwe
U
D000818:Animals; D000956:Antigens, Viral / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000175:diagnosis / Q000821:virology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D006566:Herpesviridae Infections / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D007242:Herpesvirus 1, Bovine / Q000302:isolation & purification*; D014759:Viral Envelope Proteins / Q000097:blood*; D014764:Viral Proteins
D000956:Antigens, Viral; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C046998:bovine herpesvirus type-1 glycoproteins