ORF98 of Autographa californica nucleopolyhedrosisvirus is an auxiliary factor in late gene expression.
Autographa californica nucleopolyhedrosisvirus (AcMNPV) is the type member of the family Baculoviridae. Gene expression of AcMNPV during virus infection is temporally regulated. A series of late expression factors (LEFs) are required for late gene expression to take place. A number of additional factors have also been shown to more modestly influence late gene expression. Using the LEF transient assay, we scanned the AcMNPV genome for such factors by replacing plasmids using the LEF genes with larger clones and then looked for increases in late gene expression using a reporter plasmid under the control of a late promoter. Using this approach, ORF98 was identified as having a stimulatory effect on late gene expression. The ability of ORF98 to influence early, late, and very late gene expression was established. Furthermore, tagged versions of ORF98 were localized to the nuclei of transfected cells and were shown to interact with each other as homo-oligomers. Potential roles of ORF98 in baculovirus infection are discussed.
Hefferon
Kathleen L
KL
Cornell Research Foundation, Cornell University, 20 Thornwood Drive, Ithaca, NY 14850, USA. klh22@cornell.edu
D000818:Animals; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D017924:Nucleopolyhedroviruses / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D016366:Open Reading Frames / Q000502:physiology*; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D018411:Spodoptera / Q000648:ultrastructure / Q000821:virology; D014162:Transfection / Q000379:methods; D014764:Viral Proteins / Q000235:genetics
D004268:DNA-Binding Proteins; D014764:Viral Proteins; C096108:late expression factor 2, Baculovirus; C094647:late expression factor 3, baculovirus; C084398:late expression factor 4, baculovirus