title: Comparison of PorA VR types and porA promoter sequence from Neisseria meningitidis B isolated from non-immunised children and vaccine failures immunised with a serogroup B outer membrane protein vaccine.
abstract: PorA protein is an important component of group B meningococcal protein-based vaccines. The goals of this study were: (i) to classify the non-serosubtypable strains recovered from vaccine failures and controls by porA variable region (VR) type; (ii) to investigate if point mutations of VRs of the porA gene are present in P1.19,15 strains recovered from vaccine failures and controls; (iii) to investigate if nucleotide sequence variation in the promoter region of porA gene is related to low expression of PorA protein. VR type P1.19,15 predominated in younger vaccine failures (3-47 months) compared to older failures (48-83 months). No changes in VRs of porA were observed in 46 P1.19,15 strains studied. A promoter spacer of 16bp and 10 guanidine residues in the polymeric G tract was detected in five of six strains with weak PorA expression. Overall, this study indicated that lack of antibody response was probably the major cause of low vaccine efficacy in young children.
authors.affiliation: Instituto Adolfo Lutz, Seção de Bacteriologia, Av. Dr. Arnaldo, 355, CEP 01246-902 São Paulo, Brazil.
authors.forename: Maria Cecília O
authors.initials: MC
authors.lastname: Gorla
authors.forename: Ana Paula S
authors.initials: AP
authors.lastname: Lemos
authors.forename: Claudio T
authors.initials: CT
authors.lastname: Sacchi
authors.forename: José Cássio
authors.initials: JC
authors.lastname: de Moraes
authors.forename: Lucimar G
authors.initials: LG
authors.lastname: Milagres
mesh_terms: D000907:Antibodies, Bacterial / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D004306:Dose-Response Relationship, Immunologic; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D007223:Infant; D008585:Meningitis, Meningococcal / Q000276:immunology*; D038541:Neisseria meningitidis, Serogroup B / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D017354:Point Mutation; D018272:Porins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D011401:Promoter Regions, Genetic; D014611:Vaccination
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D007135:Immunoglobulin Variable Region; D018272:Porins; C057699:porin protein, Neisseria