Utility of an in-house mycobacteriophage-based assay for rapid detection of rifampin resistance in Mycobacterium tuberculosis clinical isolates.
A rapid in-house mycobacteriophage-based assay to identify multidrug resistance by detecting the rifampin susceptibility of Mycobacterium tuberculosis in a microtiter plate format was evaluated. The sensitivity, specificity, and overall accuracy of the assay were 100%. This test is rapid to perform and suitable for widespread implementation.
Galí
N
N
Servei de Microbiologia, Hospital Universitari Germans Trias i Pujol, and Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, Barcelona, Spain.
Domínguez
J
J
Blanco
S
S
Prat
C
C
Quesada
M D
MD
Matas
L
L
Ausina
V
V
D000904:Antibiotics, Antitubercular / Q000494:pharmacology*; D024901:Drug Resistance, Multiple, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D009160:Mycobacteriophages / Q000502:physiology*; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000821:virology*; D012293:Rifampin / Q000494:pharmacology*; D013997:Time Factors; D014376:Tuberculosis / Q000382:microbiology
D000904:Antibiotics, Antitubercular; D012293:Rifampin