title: A low background high-throughput screening (HTS) fluorescence assay for lipases and esterases using acyloxymethylethers of umbelliferone.
abstract: Esters and acyloxymethyl ethers of umbelliferone were evaluated as fluorogenic substrates for lipases and esterases with respect to stability and resistance to non-specific hydrolysis. Isobutyryloxymethyl ether 1c and pivaloxymethyl ether 1d were found to be optimal substrates for enzyme assays, particularly with respect to HTS applications.
authors.affiliation: Department of Chemistry & Biochemistry, University of Bern, Freiestrasse 3, 3012, Bern, Switzerland.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Leroy
authors.forename: Nicolas
authors.initials: N
authors.lastname: Bensel
authors.forename: Jean Louis
authors.initials: JL
authors.lastname: Reymond
mesh_terms: D004353:Drug Evaluation, Preclinical; D004950:Esterases / Q000378:metabolism*; D004987:Ethers / Q000138:chemical synthesis*; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D006868:Hydrolysis; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D011549:Pseudomonas / Q000201:enzymology; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013379:Substrate Specificity; D014468:Umbelliferones / Q000138:chemical synthesis*
chemical_list: D004987:Ethers; D005456:Fluorescent Dyes; D014468:Umbelliferones; D012710:Serum Albumin, Bovine; D004950:Esterases; D008049:Lipase