title: Genes involved in beta-oxidation, energy metabolism and glyoxylate cycle are induced by Candida albicans during macrophage infection.
abstract: The ability of intracellular pathogens to cause infection is related to their capacity to survive and grow inside macrophages or in other cell types. Candida albicans latent virulence is likely to be related to a similar mechanism of avoiding killing by specialized cells and to the resulting ability to grow in such hostile environments. Using a differential display reverse transcription polymerase chain reaction technique, we have identified seven genes induced in C. albicans during macrophage phagocytosis. Sequence analyses and database searches revealed that these cDNAs coded for proteins homologous to yeast metabolic proteins. Interestingly, four of them are putative peroxisomal proteins, and two are involved in environmental signal sensing and transduction. Among the seven genes induced by C. albicans, six represent new information that were not described in other infection models.
authors.affiliation: Laboratory of Molecular Fungal Pathogenesis, Institute of Genetics and Biophysics Buzzati-Traverso-CNR, 80125 Naples, Italy.
authors.forename: Odile
authors.initials: O
authors.lastname: Prigneau
authors.forename: Amalia
authors.initials: A
authors.lastname: Porta
authors.forename: Jacques A
authors.initials: JA
authors.lastname: Poudrier
authors.forename: Sergio
authors.initials: S
authors.lastname: Colonna-Romano
authors.forename: Thierry
authors.initials: T
authors.lastname: Noël
authors.forename: Bruno
authors.initials: B
authors.lastname: Maresca
mesh_terms: D015152:Blotting, Northern; D002176:Candida albicans / Q000235:genetics* / Q000378:metabolism; D002177:Candidiasis / Q000378:metabolism; D003001:Cloning, Molecular; D004734:Energy Metabolism / Q000235:genetics* / Q000502:physiology; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal / Q000502:physiology*; D005800:Genes, Fungal / Q000235:genetics / Q000502:physiology; D006038:Glyoxylates / Q000378:metabolism*; D006801:Humans; D008264:Macrophages / Q000378:metabolism / Q000382:microbiology*; D010084:Oxidation-Reduction; D020675:Peroxisomes / Q000235:genetics / Q000378:metabolism; D010587:Phagocytosis / Q000502:physiology*; D016133:Polymerase Chain Reaction; D012331:RNA, Fungal / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D006038:Glyoxylates; D012331:RNA, Fungal