title: Purification and properties of an esterase from Aspergillus nomius HS-1 degrading ethylene glycol dibenzoate.
abstract: We isolated a fungal strain HS-1 utilizing either ethylene glycol dibenzoate or ethyl benzoate as sole source of carbon and energy, and identified it as Aspergillus nomius, based on morphological and rDNA analyses. An enzyme hydrolyzing the esters was purified from the culture supernatant of the strain to an electrophoretically homogeneous state. The enzyme was a carboxyl esterase with a monomeric structure, of which the molecular mass was about 60000, and inhibited by phenylmethylsulfonyl fluoride. The enzyme hydrolyzed various benzoate esters and p-nitrophenyl esters, and its hydrolysis rates for the most favorable substrates, n-butyl benzoate and p-nitrophenyl valerate, were comparable.
authors.affiliation: Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University, 9-1, Bunkyou 3-Chome, Fukui-shi, 910-8507, Japan. uchida@acbio2.acbio.fukui-u.ac.jp
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Uchida
authors.forename: Yuuma
authors.initials: Y
authors.lastname: Kurakata
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sawamura
authors.forename: Norio
authors.initials: N
authors.lastname: Inamura
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Kotani
authors.forename: Takayuki
authors.initials: T
authors.lastname: Uwajima
mesh_terms: D001230:Aspergillus / Q000201:enzymology*; D001565:Benzoates / Q000378:metabolism*; D004950:Esterases / Q000302:isolation & purification / Q000378:metabolism*; D004952:Esters / Q000378:metabolism; D005026:Ethylene Glycols / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D012988:Soil Microbiology; D013696:Temperature
chemical_list: D001565:Benzoates; D004952:Esters; D005026:Ethylene Glycols; D004950:Esterases