title: Implications of a polyglutamine tract in the function of the human androgen receptor.
abstract: The androgen receptor (AR) is a ligand-dependent transcription factor and belongs to the nuclear receptor family. The AR gene contains a long polymorphic CAG repeat, coding for a polyglutamine tract. In the full size AR, the deletion of the polyglutamine tract results in an increase in the transactivation through canonical AREs. However, this effect is clearly dependent on the response elements, since it is not observed on selective elements. In our assays, a deletion of the repeat positively affected the interactions of the ligand-binding domain with the amino-terminal domain as well as the recruitment of the p160 coactivator SRC-1e to the amino-terminal domain of the AR. This is reflected by an enhanced coactivation of the AR by SRC-1e.
authors.affiliation: Division of Biochemistry, Faculty of Medicine, Campus Gasthuisberg O/N, University of Leuven, O/N, Herestraat 49, B-3000, Louvain, Belgium.
authors.forename: Leen
authors.initials: L
authors.lastname: Callewaert
authors.forename: Valerie
authors.initials: V
authors.lastname: Christiaens
authors.forename: Annemie
authors.initials: A
authors.lastname: Haelens
authors.forename: Guy
authors.initials: G
authors.lastname: Verrijdt
authors.forename: Guido
authors.initials: G
authors.lastname: Verhoeven
authors.forename: Frank
authors.initials: F
authors.lastname: Claessens
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D019556:COS Cells; D051548:Histone Acetyltransferases; D006801:Humans; D066298:In Vitro Techniques; D056920:Nuclear Receptor Coactivator 1; D010455:Peptides / Q000737:chemistry* / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D017434:Protein Structure, Tertiary; D011944:Receptors, Androgen / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D025842:SUMO-1 Protein / Q000378:metabolism; D017384:Sequence Deletion; D014157:Transcription Factors / Q000378:metabolism; D015533:Transcriptional Activation
chemical_list: D010455:Peptides; D011944:Receptors, Androgen; D011994:Recombinant Proteins; D025842:SUMO-1 Protein; D014157:Transcription Factors; C097188:polyglutamine; D051548:Histone Acetyltransferases; C538859:NCOA1 protein, human; D056920:Nuclear Receptor Coactivator 1