Discovery of differentially expressed genes related to histological subtype of hepatocellular carcinoma.
Hepatocellular carcinoma (HCC) is one of the most common human malignancies in the world. To identify the histological subtype-specific genes of HCC, we analyzed the gene expression profile of 10 HCC patients by means of cDNA microarray. We proposed a systematic approach for determining the discriminatory genes and revealing the biological phenomena of HCC with cDNA microarray data. First, normalization of cDNA microarray data was performed to reduce or minimize systematic variations. On the basis of the suitably normalized data, we identified specific genes involved in histological subtype of HCC. Two classification methods, Fisher's discriminant analysis (FDA) and support vector machine (SVM), were used to evaluate the reliability of the selected genes and discriminate the histological subtypes of HCC. This study may provide a clue for the needs of different chemotherapy and the reason for heterogeneity of the clinical responses according to histological subtypes.
Lee
Dongkwon
D
Department of Chemical Engineering, Pohang University of Science and Technology, San 31 Hyoja-Dong, Pohang 790-784, Korea.
Choi
Sang Wook
SW
Kim
Myengsoo
M
Park
Jin Hyun
JH
Kim
Moonkyu
M
Kim
Jungchul
J
Lee
In-Beum
IB
D000465:Algorithms; D014408:Biomarkers, Tumor / Q000235:genetics*; D006528:Carcinoma, Hepatocellular / Q000145:classification* / Q000235:genetics* / Q000473:pathology; D003936:Diagnosis, Computer-Assisted / Q000379:methods*; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000145:classification / Q000235:genetics; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D008113:Liver Neoplasms / Q000145:classification / Q000235:genetics / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D014408:Biomarkers, Tumor