Stretching and visualizing titin molecules: combining structure, dynamics and mechanics.
The details of the global and local structure and function of titin, a giant filamentous intrasarcomeric protein are largely undiscovered. Here we discuss a combination of bulk-solution and novel single-molecule techniques that may lend unique insights into titin's molecular dynamic, structural and mechanical characteristics.
Kellermayer
Miklós S Z
MS
Department of Biophysics, University of Pécs, Medical School Pécs, Szigeti ut 12, Pécs H-7624 Hungary. miklos.kellermayer.jr@aok.pte.hu
Grama
László
L
D000818:Animals; D001696:Biomechanical Phenomena; D064211:Connectin; D019281:Dimerization; D004548:Elasticity; D005453:Fluorescence; D006801:Humans; D008846:Micromanipulation; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D008863:Microspheres; D008958:Models, Molecular; D009038:Motion; D009124:Muscle Proteins / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure*; D011485:Protein Binding; D011487:Protein Conformation; D011489:Protein Denaturation; D020033:Protein Isoforms / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D011494:Protein Kinases / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure*; D012235:Rhodamines; D013997:Time Factors
D064211:Connectin; D009124:Muscle Proteins; D020033:Protein Isoforms; D012235:Rhodamines; C577285:TTN protein, human; D011494:Protein Kinases