title: Structure and dynamics of a membrane protein in micelles from three solution NMR experiments.
abstract: Three solution NMR experiments on a uniformly (15)N labeled membrane protein in micelles provide sufficient information to describe the structure, topology, and dynamics of its helices, as well as additional information that characterizes the principal features of residues in terminal and inter-helical loop regions. The backbone amide resonances are assigned with an HMQC-NOESY experiment and the backbone dynamics are characterized by a (1)H-(15)N heteronuclear NOE experiment, which clearly distinguishes between the structured helical residues and the more mobile residues in the terminal and interhelical loop regions of the protein. The structure and topology of the helices are described by Dipolar waves and PISA wheels derived from experimental measurements of residual dipolar couplings (RDCs) and residual chemical shift anisotropies (RCSAs). The results show that the membrane-bound form of Pf1 coat protein has a 20-residue trans-membrane hydrophobic helix with an orientation that differs by about 90 degrees from that of an 8-residue amphipathic helix. This combination of three-experiments that yields Dipolar waves and PISA wheels has the potential to contribute to high-throughput structural characterizations of membrane proteins.
authors.affiliation: Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093, USA.
authors.forename: Sangwon
authors.initials: S
authors.lastname: Lee
authors.forename: Michael F
authors.initials: MF
authors.lastname: Mesleh
authors.forename: Stanley J
authors.initials: SJ
authors.lastname: Opella
mesh_terms: D016880:Anisotropy; D036022:Capsid Proteins / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D008565:Membrane Proteins / Q000737:chemistry*; D008823:Micelles; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011487:Protein Conformation; D012996:Solutions
chemical_list: D036022:Capsid Proteins; D008565:Membrane Proteins; D008823:Micelles; D012996:Solutions; C071283:coat protein, Pseudomonas phage Pf1