The maintenance and preservation of keratinophilic fungi and related dermatophytes.
A total of 239 fungi belonging to nine genera and 23 species were maintained on agar slopes in a refrigerator at +8 +/- 1 degrees C and in sterile distilled water, under liquid paraffin, dried on silica gel, in sterile soil at room temperature. A limited number has been stored by freeze-drying between 1985 and 1998. It is recommended that each isolate should be maintained by at least two of these methods. The choice of the method will depend to a large extent on the equipment and facilities available in the laboratory. It is reported that simple methods can be used for preservation of keratinophilic fungi and related dermatophytes.
Deshmukh
S K
SK
Department of Applied Microbiology and Biotechnology, Dr H.S. Gour Vishwavidyalaya, Sagar, India. sdeshmukh@nicholaspiramal.co.in
D003883:Arthrodermataceae / Q000502:physiology*; D015925:Cryopreservation; D005658:Fungi / Q000502:physiology*; D007633:Keratins / Q000378:metabolism; D008899:Mineral Oil; D012034:Refrigeration; D058428:Silica Gel; D012822:Silicon Dioxide; D012988:Soil Microbiology; D013997:Time Factors; D014867:Water
D014867:Water; D058428:Silica Gel; D007633:Keratins; D012822:Silicon Dioxide; D008899:Mineral Oil