Understanding the molecular responses to hypoxia using Drosophila as a genetic model.
We have previously discovered that Drosophila melanogaster could recover from extended periods of anoxia (0% oxygen) with no apparent consequential injuries. We have since employed forward and reverse genetic approaches to decipher the molecular basis for anoxia tolerance. In so doing, we have identified several independent mutant lines that demonstrated increased sensitivity to anoxia. Characterization of one of these mutants resulted in the identification of a dADAR gene that plays a role in the sensitivity to low levels of oxygen. We have also used microarrays to study all known Drosophila genes, the expression of which may be altered by hyoxia. Microarrays experiments have generated a large body of information that is being currently analyzed. We believe that these undertakings will provide insight into the genetic mechanisms of hypoxia tolerance and ischemic injuries.
Farahani
Reza
R
Department of Pediatrics, Albert Einstein College of Medicine of Yeshiva University, The Children's Hospital at Montefiore, Jack and Pearl Resnick Campus, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
Haddad
Gabriel G
GG
D000243:Adenosine Deaminase / Q000235:genetics; D000818:Animals; D004330:Drosophila / Q000235:genetics / Q000502:physiology*; D005786:Gene Expression Regulation; D017344:Genes, Insect; D000860:Hypoxia / Q000235:genetics*; D008957:Models, Genetic; D020411:Oligonucleotide Array Sequence Analysis; D010100:Oxygen / Q000378:metabolism*; D016601:RNA-Binding Proteins
D016601:RNA-Binding Proteins; C567955:ADARB1 protein, human; D000243:Adenosine Deaminase; D010100:Oxygen