title: Design of artificial nucleobases for the recognition of the AT inversion by triple-helix forming oligonucleotides: a structure-stability relationship study and neighbour bases effect.
abstract: We report herein on the synthesis, the incorporation into triplex forming oligonucleotides (TFO) and the recognition properties of a series of synthetic nucleosides designed for the specific recognition of an inverted A x T base pair in a pyrimidine triple helix motif. These analogues were designed on the basis of the results obtained with our previously reported compounds S and B(t), in order to define a structure-stability relationship. We report also on the chemical nature effect of the bases flanking S in the case of S-containing TFOs, in order to get further informations about the recognition process within the A x TxS triplet. This study establishes guidelines for the conception of more potent analogues for the recognition of both A x T and G x C inverted base pairs.
authors.affiliation: Laboratoire de Biophysique, UR 565 INSERM, UMR 8646 CNRS, Muséum National d'Histoire Naturelle, 43 rue Cuvier 75231 Paris Cédex 05, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Guianvarc'h
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Fourrey
authors.forename: Rosalie
authors.initials: R
authors.lastname: Maurisse
authors.forename: Jian-Sheng
authors.initials: JS
authors.lastname: Sun
authors.forename: Rachid
authors.initials: R
authors.lastname: Benhida
mesh_terms: D000225:Adenine; D020029:Base Pairing; D001483:Base Sequence; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000138:chemical synthesis; D018583:Pliability; D013941:Thymine
chemical_list: D009838:Oligodeoxyribonucleotides; D009943:Organophosphorus Compounds; C434331:phosphoramidite; D000225:Adenine; D013941:Thymine