title: [Hematopoietic reconstitution in early period after autologous and allogeneic peripheral blood stem cell transplantation].
abstract: OBJECTIVE
  To investigate the ratio and amount of CD34+ cells and their subsets in mobilized peripheral blood (MPB) and leukapheresis products in patients receiving autologous peripheral blood stem cell transplantation (auto-PBSCT) and in allo-PBSCT donors, and clarify the role of CD34+ cells in post-transplantation hematopoietic reconstitution.
  
  
  METHODS
  Sixteen patients receiving auto-PBSCT and 24 allo-PBSCT donors were studied for CD34+ and CD34+CD38- cell ratios in the 115 MPB and their leukapheresis product samples by way of flow cytometry, and the time of hematopoietic recovery was observed in the two groups.
  
  
  RESULTS
  The CD34+ cell ratio in the MPB samples and leukapheresis products was significantly lower in the donors than in auto-PBSCT patients (P<0.05), but CD34+CD38- cell ratio showed no obvious difference between the two groups. The amount of mononuclear cells (MNCs) received by allo-PBSCT patients was much more than that received by auto-PBSCT patients (P<0.01). Similar average times for post-transplant neutrophils recovery to over 0.5x10(9)/L and platelets to over 2 x 10(10)/L (P>0.05) were observed between the groups, and in both of them, the recovery was related to the amount of MNCs, CD34+ and CD34+/CD38- cell infusion.
  
  
  CONCLUSIONS
  Irrespective of auto-PBSCT or allo-PBSCT, the number of CD34+ and CD34+CD38-cells infused after the transplantation is the key factor to influence the course of hemopoietic reconstitution.
authors.affiliation: Department of Hematology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. wyq@fimmu.com
authors.forename: Yong-qiang
authors.initials: YQ
authors.lastname: Wei
authors.forename: Ru
authors.initials: R
authors.lastname: Feng
authors.forename: Zheng-shan
authors.initials: ZS
authors.lastname: Yi
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.forename: Qi-fa
authors.initials: QF
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.forename: Fan-yi
authors.initials: FY
authors.lastname: Meng
authors.forename: Shu-yun
authors.initials: SY
authors.lastname: Zhou
mesh_terms: D036541:ADP-ribosyl Cyclase / Q000032:analysis; D051997:ADP-ribosyl Cyclase 1; D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34 / Q000032:analysis; D002648:Child; D005260:Female; D006410:Hematopoiesis; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins; D008875:Middle Aged; D036102:Peripheral Blood Stem Cell Transplantation; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34; D008562:Membrane Glycoproteins; D036541:ADP-ribosyl Cyclase; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1