title: Establishment of transgenic mice carrying the gene of human nuclear receptor NR5A2 (hB1F).
abstract: OBJECTIVE
  Human hepatitis B virus enhancer II B1 binding factor (hB1F) was cloned and characterized as a novel member of the Ftz-F1 (NR5A) nuclear receptor subfamily. Although progresses have recently been made, its biological function remains largely unidentified. The aim of this study was to establish an hB1F transgenic mouse model to promote the functional study of hB1F.
  
  
  METHODS
  Transgene fragments were microinjected into fertilized eggs of mice. The manipulated embryos were transferred into the oviducts of pseudopregnant female mice. The offsprings were identified by PCR and Southern blot analysis. Transgene expression was analyzed with RT-PCR and Western blot analysis. Transgenic founder mice were used to establish transgenic mouse lineages. The F1 and F2 mice were identified by PCR analysis.
  
  
  RESULTS
  Seven mice were identified as carrying copies of transgene. RT-PCR and Western blotting results showed that the transgene was expressed in heart, liver, lung, kidney and stomach in one of the transgenic mouse lineages. Genetic analysis of the transgenic mice demonstrated that the transgene was integrated into the chromosome at a single site, and was transmitted stably.
  
  
  CONCLUSIONS
  In this study we established an hB1F transgenic mouse model, which will facilitate the investigation of the biological function of hB1F in vivo.
authors.affiliation: Department of Medical Genetics, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China.
authors.forename: Shui-Liang
authors.initials: SL
authors.lastname: Wang
authors.forename: Hua
authors.initials: H
authors.lastname: Yang
authors.forename: You-Hua
authors.initials: YH
authors.lastname: Xie
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Jian-Zhong
authors.initials: JZ
authors.lastname: Li
authors.forename: Long
authors.initials: L
authors.lastname: Wang
authors.forename: Zhu-Gang
authors.initials: ZG
authors.lastname: Wang
authors.forename: Ji-Liang
authors.initials: JL
authors.lastname: Fu
mesh_terms: D000818:Animals; D004268:DNA-Binding Proteins; D005260:Female; D015870:Gene Expression; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic / Q000235:genetics*; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics*; D014018:Tissue Distribution; D014157:Transcription Factors; D019076:Transgenes
chemical_list: D004268:DNA-Binding Proteins; C082631:NR5A2 protein, human; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors