[Effect of triglycerides on rat islet cell function in vitro].
OBJECTIVE
To study the effect of triglycerides (TGs) on the function of in vitro cultured rat islet cells.


METHODS
SD rat islet cells were isolated for monolayer cell culture and treated with TGs at different concentrations (0, 0.5, 2.5, 5.0, 10.0 mmol/L) for 72 h. Insulin level in the cell culture media was measured after glucose loading test, and the deposition of fat droplets in the islet cells observed by oil red O-staining.


RESULTS
No obvious effect was observed after a 72-h incubation of the islet cells with TGs at low glucose level (2.8 mmol/L), while with the stimulation of high-level glucose (27.8 mmol/L), the insulin secretion was significantly inhibited by TGs of higher concentrations (5.0, 10.0 mmol/L). The deposition of fat droplets was found in the islet cells after incubation with TGs, with TGs of higher concentrations.


CONCLUSIONS
TGs induces deposition of fat droplets in islet cells in vitro, and may inhibit the insulin-secreting function of the cells.
Zhu
Zi-ying
ZY
Department of Endocrinology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Xue
Yao-ming
YM
Jiang
Yi-zhen
YZ
D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D050356:Lipid Metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014280:Triglycerides / Q000494:pharmacology*
D007328:Insulin; D014280:Triglycerides