title: Identification of Arabidopsis rat mutants.
abstract: Limited knowledge currently exists regarding the roles of plant genes and proteins in the Agrobacterium tumefaciens-mediated transformation process. To understand the host contribution to transformation, we carried out root-based transformation assays to identify Arabidopsis mutants that are resistant to Agrobacterium transformation (rat mutants). To date, we have identified 126 rat mutants by screening libraries of T-DNA insertion mutants and by using various "reverse genetic" approaches. These mutants disrupt expression of genes of numerous categories, including chromatin structural and remodeling genes, and genes encoding proteins implicated in nuclear targeting, cell wall structure and metabolism, cytoskeleton structure and function, and signal transduction. Here, we present an update on the identification and characterization of these rat mutants.
authors.affiliation: Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-1392, USA.
authors.forename: Yanmin
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authors.lastname: Zhu
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authors.lastname: Nam
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authors.lastname: Mysore
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authors.lastname: Crane
authors.forename: Kui
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authors.lastname: Lin
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authors.lastname: Larcher
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authors.lastname: Gelvin
authors.forename: Marnie
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authors.lastname: Knue
authors.forename: Cynthia
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authors.lastname: Ramos
authors.forename: Xiaowen
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authors.lastname: Zhao
authors.forename: Susan J
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authors.lastname: Davis
authors.forename: Sang-Ic
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authors.lastname: Kim
authors.forename: C T
authors.initials: CT
authors.lastname: Ranjith-Kumar
authors.forename: Yoo-Jin
authors.initials: YJ
authors.lastname: Choi
authors.forename: Vipin K
authors.initials: VK
authors.lastname: Hallan
authors.forename: Sudip
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authors.lastname: Chattopadhyay
authors.forename: Xiangzhen
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authors.lastname: Sui
authors.forename: Alicja
authors.initials: A
authors.lastname: Ziemienowicz
authors.forename: Ann G
authors.initials: AG
authors.lastname: Matthysse
authors.forename: Vitaly
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authors.lastname: Citovsky
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authors.lastname: Hohn
authors.forename: Stanton B
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authors.lastname: Gelvin
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000502:physiology; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics; D001483:Base Sequence; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D004798:Enzymes / Q000235:genetics; D009154:Mutation; D018517:Plant Roots / Q000378:metabolism; D016133:Polymerase Chain Reaction; D016372:RNA, Antisense / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D004798:Enzymes; D016372:RNA, Antisense; D034741:RNA, Small Interfering; C036483:T-DNA