title: Characterization of lipoproteins from the turtle, Trachemys scripta elegans, in fasted and fed states.
abstract: The lipid and apolipoprotein composition of VLDL, IDL, LDL, HDL(2) and HDL(3) were examined in the turtle, Trachemys scripta elegans, in fasted and fed states. The lipid composition of turtle lipoproteins was very similar to their human counterparts. The major apolipoprotein found in LDL, IDL and VLDL, which has a molecular weight of approximately 550 kD, is a homologue of apoB100. The major apolipoprotein found in both HDL(2) and HDL(3), has a molecular weight of 28-kD and is homologous to human apoA-I. HDL(3) also contains a 6.5 kD protein that is homologous to apoA-II, while HDL(2) has two low molecular weight proteins of 6 kD and 7 kD which are also found on the triglyceride rich lipoproteins (TRL). The 7 kD protein is homologous to apoC-III, while the 6 kD protein has a similar size and distribution as apoC-II or apoC-I. In addition, HDL(2) also possesses a protein of 15.8 kD that has no obvious mammalian homologue. In both size and apolipoprotein composition, turtle HDL(2) resembles human HDL(2b) while turtle HDL(3) resembles human HDL(3). In the fasted state, turtles contained very little TRL. When fed a high fat diet, the amount of IDL and LDL sized particles increased significantly.
authors.affiliation: Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA. will@udel.edu
authors.forename: William
authors.initials: W
authors.lastname: Cain
authors.forename: Li
authors.initials: L
authors.lastname: Song
authors.forename: Gregory
authors.initials: G
authors.lastname: Stephens
authors.forename: David
authors.initials: D
authors.lastname: Usher
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005215:Fasting; D008074:Lipoproteins / Q000097:blood* / Q000737:chemistry / Q000145:classification; D008969:Molecular Sequence Data; D014426:Turtles / Q000378:metabolism*
chemical_list: D008074:Lipoproteins