Effect of Varidase (streptodornase) on biofilm formed by Pseudomonas aeruginosa.
The biofilm of Pseudomonas aeruginosa could be removed by Varidase (streptodornase) that was used as defibrinating drug. After cultivating the biofilm on a silicone chip in a modified alginate-producing medium in vitro, it was treated with Varidase or DNase I. In both treatments, the biofilm was removed depending on the concentration of the reagents. Varidase was apparently effective under the condition that the biofilm was exposed to more than 625 U/ml (a quarter of the concentration of the medical use) for 3 h, twice, at 37 degrees C. The result of this experiment indicates that (a) the DNases, DNase I and Varidase, were effective in destroying the biofilm of P. aeruginosa in vitro, and (b) in a clinical field, Varidase could be useful for P. aeruginosa focal infection, such as urinary tract infection, by removing the biofilm.
Nemoto
Ken
K
Department of Oral Microbiology, Tokushima University School of Dentistry, Tokushima, Japan. nemoto@dent.tokushima-u.ac.jp
Hirota
Katsuhiko
K
Murakami
Keiji
K
Taniguti
Kazuko
K
Murata
Hiromi
H
Viducic
Darija
D
Miyake
Yoichiro
Y
D000464:Alginates; D018441:Biofilms; D018929:Cell Culture Techniques; D003470:Culture Media; D003850:Deoxyribonuclease I / Q000494:pharmacology* / Q000627:therapeutic use; D004305:Dose-Response Relationship, Drug; D006801:Humans; D011552:Pseudomonas Infections / Q000188:drug therapy*; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D014552:Urinary Tract Infections / Q000188:drug therapy*
D000464:Alginates; D003470:Culture Media; D003850:Deoxyribonuclease I