Screening adenoviruses in stool samples: evaluation of a genus-specific monoclonal antibody based enzyme immunoassay.
To screen fecal samples for adenovirus antigens a genus-specific monoclonal antibody based enzyme immunoassay was developed. In a comparative analysis with commercial latex agglutination test, high sensitivity was demonstrated. The assay did not detect other viruses usually found in faeces suggesting its specificity. One hundred and eighty stool samples collected in Baranya County were tested and 13 (7.2%) of them showed reactivity. The application of our immunoassay combined with other, more sophisticated methods may help us to determine the serotype specificity of these adenovirus isolates and assess the importance of adenoviruses in viral gastroenteritis.
Bányai
K
K
Regional Laboratory of Virology, Baranya County Institute, State Public Health Service, Szabadságút 7, P.O. Box 47, H-7601 Pécs, Hungary.
Máté
Zsanett
Z
Adám
Eva
E
Uj
Mária
M
Nász
I
I
Szücs
G
G
D000258:Adenovirus Infections, Human / Q000175:diagnosis / Q000821:virology; D000260:Adenoviruses, Human / Q000276:immunology / Q000302:isolation & purification*; D000293:Adolescent; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D000918:Antibody Specificity; D002648:Child; D002675:Child, Preschool; D005243:Feces / Q000821:virology*; D005759:Gastroenteritis / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D007231:Infant, Newborn; D007841:Latex Fixation Tests; D008403:Mass Screening; D012703:Serotyping; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral