OptiPrep density gradient solutions for nonmammalian organelles.
Any density gradient for the isolation of nonmammalian organelles should ideally only expose the sedimenting biological particles to an increasing concentration of the gradient solute. Thus they will experience only an increasing density and viscosity, other parameters such as osmolality, pH, ionic strength and the concentration of important additives (such as EDTA and DTT) should remain as close to constant as possible. This Protocol Article describes the strategies for the dilution of OptiPrep in order to prepare such solutions for organelles and membranes from nonmammalian sources such as yeast.
Graham
John M
JM
School of Biomolecular Sciences, Liverpool John Moores University. john@jgrescon.fsbusiness.co.uk
D002021:Buffers; D002458:Cell Fractionation / Q000379:methods*; D002499:Centrifugation, Density Gradient / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D015388:Organelles; D009994:Osmolar Concentration; D010944:Plants / Q000648:ultrastructure; D012996:Solutions / Q000737:chemistry; D015003:Yeasts / Q000648:ultrastructure
D002021:Buffers; D012996:Solutions