title: Halobacillus salinus sp. nov., isolated from a salt lake on the coast of the East Sea in Korea.
abstract: A Gram-positive, rod-shaped, endospore-forming, halophilic bacterium (strain HSL-3(T)) was isolated from a salt lake near Hwajinpo beach on the East Sea in Korea and was subjected to a polyphasic taxonomic study. Strain HSL-3(T) grew optimally in the presence of 2-10% (w/v) NaCl. Strain HSL-3(T) showed poor growth in the absence of NaCl and grew in the presence of less than 23% NaCl. The cell wall peptidoglycan type of strain HSL-3(T) was A4beta based on L-Orn-D-Asp. The predominant menaquinone found in strain HSL-3(T) was menaquinone-7 (MK-7). Strain HSL-3(T) had a cellular fatty acid profile containing large amounts of branched fatty acids; the major fatty acids were anteiso-C(15 : 0), iso-C(15 : 0) and iso-C(16 : 0). The DNA G + C content of strain HSL-3(T) was 45 mol%. Phylogenetic analysis based on 16S rDNA sequences showed that strain HSL-3(T) falls within the radiation of the cluster comprising Halobacillus species. Strain HSL-3(T) exhibited levels of 16S rDNA similarity of 97.4-98.4% to the type strains of Halobacillus species. Levels of DNA-DNA relatedness between strain HSL-3(T) and the type strains of all validly named Halobacillus species were in the range 7.3-9.2%. On the basis of phenotypic and phylogenetic data and the genomic distinctiveness, strain HSL-3(T) (= KCCM 41590(T) = JCM 11546(T)) should be placed in the genus Halobacillus as the type strain of a novel species, for which the name Halobacillus salinus sp. nov. is proposed.
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
authors.affiliation: Department of Food and Life Science, Sungkyunkwan University, Chunchun-dong 300, Jangan-gu, Suwon, Korea.
authors.forename: Kook Hee
authors.initials: KH
authors.lastname: Kang
authors.affiliation: National Research Laboratory of Molecular Ecosystematics, Institute of Probionic, Probionic Corporation, Bio-venture Center, KRIBB, Yusong, Taejon, Korea.|Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
authors.forename: Yong-Ha
authors.initials: YH
authors.lastname: Park
mesh_terms: D001406:Bacillaceae / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005618:Fresh Water / Q000382:microbiology; D007723:Korea; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism
chemical_list: D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride