title: Components of cigarette smoke inhibit expansion of oocyte-cumulus complexes from porcine follicles.
abstract: The role of alkaloids in cigarette smoke was investigated in the cumulus expansion of oocyte-cumulus complexes (OCC) isolated from large antral porcine follicles. Suppression of the cumulus expansion stimulated by FSH was observed in the presence of different concentration of cadmium, anabasine and nicotine but not its metabolite cotinine. There were comparable inhibitory effects of cadmium and nicotine on the synthesis and accumulation of hyaluronic acid in the cell/matrix compartment of OCC. The inhibitory effect of tested compounds on the cumulus expansion was accompanied by decreased progesterone synthesis by cumulus cells during 42 h incubation of OCC with FSH. The results suggest that cigarette smoking may affect intrafollicular processes, which are responsible for normal ovulation and fertilization.
authors.affiliation: Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava, Slovak Republic. ueenkole@savba.sk
authors.forename: S
authors.initials: S
authors.lastname: Vrsanská
authors.forename: E
authors.initials: E
authors.lastname: Nagyová
authors.forename: A
authors.initials: A
authors.lastname: Mlynarcíková
authors.forename: M
authors.initials: M
authors.lastname: Ficková
authors.forename: J
authors.initials: J
authors.lastname: Kolena
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000691:Anabasine / Q000494:pharmacology; D000818:Animals; D002104:Cadmium / Q000494:pharmacology; D003367:Cotinine / Q000494:pharmacology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism; D006820:Hyaluronic Acid / Q000096:biosynthesis*; D009538:Nicotine / Q000494:pharmacology; D006080:Ovarian Follicle / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D011374:Progesterone / Q000096:biosynthesis* / Q000378:metabolism; D013552:Swine; D014026:Nicotiana / Q000737:chemistry
chemical_list: D000470:Alkaloids; D002104:Cadmium; D011374:Progesterone; D009538:Nicotine; D005640:Follicle Stimulating Hormone; D006820:Hyaluronic Acid; D003367:Cotinine; D000691:Anabasine