Comparative study of protective effects of chitin, chitosan, and N-acetyl chitohexaose against Pseudomonas aeruginosa and Listeria monocytogenes infections in mice.
We conducted a comparative study of the protective effects of chitin, chitosan, and N-acetyl chitohexaose (NACOS-6) against mice infected intravenously or intraperitoneally with Pseudomonas aeruginosa and Listeria monocytogenes. Mice pretreated with chitin, chitosan, and NACOS-6 showed resistance to intraperitoneal infections by both microbes. Only mice pretreated with chitin and chitosan showed resistance to intravenous infections by both microbes. The number, active oxygen generation, and myeloperoxidase activity of peritoneal exudate cells (PEC) in the chitin, chitosan, and NACOS-6-treated mice were greater than those of the untreated mice. Also, these PEC factors from mice pretreated with chitin and chitosan were greater than those from the NACOS-6-treated mice.
Okawa
Yoshio
Y
Second Department of Hygienic Chemistry, Tohoku Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai, Miyagi 981-8558, Japan. okawa@tohoku-pharm.ac.jp
Kobayashi
Makiko
M
Suzuki
Shigeo
S
Suzuki
Masuko
M
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000627:therapeutic use*; D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology / Q000201:enzymology; D002686:Chitin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000627:therapeutic use*; D048271:Chitosan; D004195:Disease Models, Animal; D005122:Exudates and Transudates / Q000166:cytology / Q000201:enzymology; D007274:Injections, Intraperitoneal; D008089:Listeria monocytogenes / Q000472:pathogenicity; D008088:Listeriosis / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D051379:Mice; D008815:Mice, Inbred Strains; D009844:Oligosaccharides / Q000008:administration & dosage / Q000627:therapeutic use*; D009195:Peroxidase / Q000378:metabolism; D011552:Pseudomonas Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D011550:Pseudomonas aeruginosa / Q000472:pathogenicity
D000276:Adjuvants, Immunologic; D009844:Oligosaccharides; D002686:Chitin; C056529:N-acetylchitohexaose; D048271:Chitosan; D009195:Peroxidase