Quantification of cell adhesion force with AFM: distribution of vitronectin receptors on a living MC3T3-E1 cell.
Distribution of vitronectin (VN) receptors on a living murine osteoblastic cell was successfully measured by atomic force microscopy (AFM). First, the distribution of the integrin beta(5) subunit which constitutes a part of the VN receptor on the cell was confirmed by conventional immunohistochemistry after fixing the cell. To visualize the distribution of the receptor on a living cell by an independent and potentially a more quantitative method, the AFM was used with a microbead attached to the cantilever tip to increase the area of contact and VN was immobilized on the microbead. Force measurements were then performed over a large area of a living murine osteoblastic cell using the microbead covered with VN.
Kim
H
H
Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama 226-8501, Japan. hkim@bio.titech.ac.jp
Arakawa
Hideo
H
Osada
Toshiya
T
Ikai
Atsushi
A
D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D007150:Immunohistochemistry; D039641:Integrin beta Chains / Q000378:metabolism; D051379:Mice; D018625:Microscopy, Atomic Force / Q000379:methods*; D010006:Osteoblasts / Q000378:metabolism / Q000648:ultrastructure*; D019030:Receptors, Vitronectin / Q000378:metabolism / Q000648:ultrastructure*; D019096:Vitronectin / Q000378:metabolism*
D039641:Integrin beta Chains; D019030:Receptors, Vitronectin; D019096:Vitronectin; C084344:integrin beta5