title: Human neutrophil antigen-4a gene frequencies in an Australian population, determined by a new polymerase chain reaction method using sequence-specific primers.
abstract: Human neutrophil antigen-4a (HNA-4a) is a high-frequency (99% in the USA) neutrophil antigen, which has recently been linked to a case of alloimmune neonatal neutropenia. We have devised a new polymerase chain reaction sequence-specific primer (PCR-SSP) method to assess HNA-4a genotype, and used it to determine the HNA-4a gene frequencies in an Australian population. The gene frequency was found to be 0.906, which is the same as in the American population. The PCR-SSP genotyping method perfectly correlates with serological phenotyping and is efficient for screening large numbers of samples.
authors.affiliation: Queensland Institute of Medical Research, Australian Red Cross Blood Service, Brisbane, Queensland, Australia.
authors.forename: H D
authors.initials: HD
authors.lastname: Clague
authors.forename: Y L
authors.initials: YL
authors.lastname: Fung
authors.forename: R M
authors.initials: RM
authors.lastname: Minchinton
mesh_terms: D001315:Australia / Q000453:epidemiology; D001483:Base Sequence; D017931:DNA Primers; D005787:Gene Frequency; D005838:Genotype; D006098:Granulocytes; D006801:Humans; D007519:Isoantigens / Q000235:genetics*; D017720:Molecular Epidemiology; D009503:Neutropenia / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers; D007519:Isoantigens