Knockout of p75NTR does not alter the viability of striatal neurons following a metabolic or excitotoxic injury.
Following metabolic or excitotoxic injury to the striatum, there is de novo expression of the low-affinity p75 neurotrophin receptor (p75NTR). The novel expression of this pan neurotrophin receptor in rodents occurs within the lesion core and surrounding area, creating a division between viable and nonviable tissue. The present series of experiments sought to elucidate whether the p75NTR expression seen following metabolic and excitotoxic injury alters neuronal viability within the striatum. Toward this end, we compared the extent of striatal lesion created with quinolinic acid (QA) or 3-nitropropionic acid (3-NP) in p75NTR null and wild-type mice. Using stereological techniques, we found that the lesion volume and neuronal cell counts between p75NTR null and wild-type mice were similar 1, 2, and 4 weeks post-QA or -3-NP lesion. The results indicate that the expression of p75NTR within reactive astrocytes in the mouse striatum is not a key factor in protecting neuronal cell death following metabolic and excitotoxic insults.
Hanbury
Rose
R
Research Center for Brain Repair and Department of Neurological Sciences, Rush Presbyterian Medical Center, Chicago, IL 60612, USA.
Chen
Er-Yun
EY
Wuu
Joanne
J
Kordower
Jeffrey H
JH
D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism; D001930:Brain Injuries / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D016923:Cell Death / Q000187:drug effects / Q000235:genetics; D002470:Cell Survival / Q000235:genetics; D004734:Energy Metabolism / Q000187:drug effects; D005911:Gliosis / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D017072:Neostriatum / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D009410:Nerve Degeneration / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D009498:Neurotoxins / Q000494:pharmacology; D009574:Nitro Compounds; D011422:Propionates / Q000494:pharmacology; D017378:Quinolinic Acid / Q000494:pharmacology; D020800:Receptor, Nerve Growth Factor; D017475:Receptors, Nerve Growth Factor / Q000172:deficiency* / Q000235:genetics
D009498:Neurotoxins; D009574:Nitro Compounds; D011422:Propionates; D020800:Receptor, Nerve Growth Factor; D017475:Receptors, Nerve Growth Factor; D017378:Quinolinic Acid; C015392:3-nitropropionic acid