Targeted disruption of Aldh1a1 (Raldh1) provides evidence for a complex mechanism of retinoic acid synthesis in the developing retina.
Genetic studies have shown that retinoic acid (RA) signaling is required for mouse retina development, controlled in part by an RA-generating aldehyde dehydrogenase encoded by Aldh1a2 (Raldh2) expressed transiently in the optic vesicles. We examined the function of a related gene, Aldh1a1 (Raldh1), expressed throughout development in the dorsal retina. Raldh1(-/-) mice are viable and exhibit apparently normal retinal morphology despite a complete absence of Raldh1 protein in the dorsal neural retina. RA signaling in the optic cup, detected by using a RARE-lacZ transgene, is not significantly altered in Raldh1(-/-) embryos at embryonic day 10.5, possibly due to normal expression of Aldh1a3 (Raldh3) in dorsal retinal pigment epithelium and ventral neural retina. However, at E16.5 when Raldh3 is expressed ventrally but not dorsally, Raldh1(-/-) embryos lack RARE-lacZ expression in the dorsal retina and its retinocollicular axonal projections, whereas normal RARE-lacZ expression is detected in the ventral retina and its axonal projections. Retrograde labeling of adult Raldh1(-/-) retinal ganglion cells indicated that dorsal retinal axons project to the superior colliculus, and electroretinography revealed no defect of adult visual function, suggesting that dorsal RA signaling is unnecessary for retinal ganglion cell axonal outgrowth. We observed that RA synthesis in liver of Raldh1(-/-) mice was greatly reduced, thus showing that Raldh1 indeed participates in RA synthesis in vivo. Our findings suggest that RA signaling may be necessary only during early stages of retina development and that if RA synthesis is needed in dorsal retina, it is catalyzed by multiple enzymes, including Raldh1.
Fan
Xiaohong
X
OncoDevelopmental Biology Program. Center for Neuroscience and Aging, Burnham Institute, La Jolla, California 92037, USA.
Molotkov
Andrei
A
Manabe
Shin-Ichi
S
Donmoyer
Christine M
CM
Deltour
Louise
L
Foglio
Mario H
MH
Cuenca
Arnold E
AE
Blaner
William S
WS
Lipton
Stuart A
SA
Duester
Gregg
G
D000445:Aldehyde Oxidoreductases / Q000235:genetics* / Q000502:physiology*; D000818:Animals; D015139:Blotting, Southern; D002851:Chromatography, High Pressure Liquid; D004596:Electroretinography; D005091:Exons; D005838:Genotype; D006579:Heterozygote; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008099:Liver / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D008822:Mice, Transgenic; D008957:Models, Genetic; D012160:Retina / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D050697:Retinal Dehydrogenase; D015398:Signal Transduction; D013997:Time Factors; D019076:Transgenes; D014212:Tretinoin / Q000378:metabolism*
D014212:Tretinoin; D000445:Aldehyde Oxidoreductases; D050697:Retinal Dehydrogenase