Effect of oxotremorine on resting membrane potential and cell volume in skeletal muscle fibers in rats after in vivo blockade of NO-synthase.
Denervation of rat phrenic muscle or block of NO-synthase in vivo increased the cross-section area of muscle fibers and decreased membrane resting potential. Oxotremorine prevented the development of denervation-induced or denervation-like (i.e. induced by NO-synthase blockade) membrane depolarization and increase of the cross-sectional area of muscle fibers. Pirenzepine abolished the effects of oxotremorine. It was concluded that non-quantal acetylcholine can be involved in the regulation of skeletal muscle fiber volume via activation of M1 muscarinic receptors followed by NO synthesis.
Khairova
R A
RA
Kazan State Medical University, Russian Academy of Sciences, Russia.
Malomuzh
A I
AI
Naumenko
N V
NV
Urazaev
A Kh
AKh
D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D048429:Cell Size; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects* / Q000502:physiology; D018721:Muscarinic Agonists / Q000494:pharmacology*; D018727:Muscarinic Antagonists / Q000494:pharmacology; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000166:cytology* / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010095:Oxotremorine / Q000494:pharmacology*; D010890:Pirenzepine / Q000494:pharmacology; D051381:Rats
D018721:Muscarinic Agonists; D018727:Muscarinic Antagonists; D010890:Pirenzepine; D010095:Oxotremorine; D019001:Nitric Oxide Synthase; D000109:Acetylcholine