Hybridisation of short DNA molecules investigated with in situ atomic force microscopy.
By introducing the complementary DNA (cDNA) strand to a molecular layer of short single stranded DNA (ssDNA), immobilised on a gold surface, we have investigated hybridisation between the two DNA strands through the technique of in situ atomic force microscopy (AFM). Before introduction of cDNA, the ssDNA molecular layer was modulated with the spacer molecule mercaptohexanol (MCH), which makes the ssDNA molecules more accessible for hybridisation. With in situ AFM, we have monitored the formation of a smooth, mixed molecular layer containing ssDNA and MCH. Furthermore, the hybridisation between the two DNA strands has been studied. Introduction of the cDNA strand resulted in an increase in smoothness and thickness of the molecular layer. Both the increase in order and thickness of the molecular layer can be expected if hybridisation occurs, since double stranded DNA molecules have a more rigid and elongated structure than ssDNA molecules.
Holmberg
M
M
Danish Institute of Fundamental Metrology (DFM), Matematiktorvet, B307, 2800 Kgs., Lyngby, Denmark. mh@dfm.dtu.dk
Kühle
A
A
Garnaes
J
J
Boisen
A
A
D015374:Biosensing Techniques / Q000379:methods; D018076:DNA, Complementary / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry*; D006046:Gold; D000441:Hexanols / Q000737:chemistry; D018625:Microscopy, Atomic Force / Q000379:methods*; D009693:Nucleic Acid Hybridization / Q000379:methods; D013499:Surface Properties
D018076:DNA, Complementary; D004277:DNA, Single-Stranded; D000441:Hexanols; D006046:Gold