Metal incorporation in modified PNA duplexes.
Substitution of bipyridine for a nucleobase leads to modified peptide nucleic acid (PNA) single strands that are bridged in the presence of Ni2+ into a duplex containing a combination of hydrogen and coordinative bonds. CD experiments demonstrate that the duplex adopts a structure similar to that of an unmodified 10-bp PNA duplex, and UV melting experiments show a very sensitive dependence of the duplex stability on the substitution of a nucleobase pair with a pair of ligands or a metal-ligand alternative base pair.
Popescu
Delia-Laura
DL
Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213, USA.
Parolin
Terry J
TJ
Achim
Catalina
C
D015082:2,2'-Dipyridyl / Q000737:chemistry; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D019216:Metals, Heavy / Q000737:chemistry*; D009532:Nickel / Q000737:chemistry; D010165:Palladium / Q000737:chemistry; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
D019216:Metals, Heavy; D020135:Peptide Nucleic Acids; D010984:Platinum; D015082:2,2'-Dipyridyl; D010165:Palladium; D009532:Nickel; D004247:DNA