title: Use of salt hydrate pairs to control water activity for enzyme catalysis in ionic liquids.
abstract: Salt hydrate pairs were used to control water activity in the ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate. It was shown that salt hydrate pairs behave essentially the same in ionic liquids as they do in organic solvents as long as they do not dissolve. Initial rate-water activity profiles were prepared for the immobilized Candida antarctica lipase catalyzed synthesis of 2-ethylhexyl methacrylate. The ability to use salt hydrate pairs for the control of water activity in ionic liquids should allow for improved comparison of enzyme activity and specificity in ionic liquids and conventional solvents.
authors.affiliation: McGowan Institute for Regenerative Medicine, Suite 200, 100 Technology Drive, University of Pittsburgh, Pittsburgh, Pennsylvania 15219, USA.
authors.forename: Jason A
authors.initials: JA
authors.lastname: Berberich
authors.forename: Joel L
authors.initials: JL
authors.lastname: Kaar
authors.forename: Alan J
authors.initials: AJ
authors.lastname: Russell
mesh_terms: D002384:Catalysis; D004789:Enzyme Activation; D004800:Enzymes, Immobilized; D004951:Esterification; D005656:Fungal Proteins; D000441:Hexanols / Q000737:chemistry*; D007093:Imidazoles / Q000737:chemistry*; D007477:Ions / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D008689:Methacrylates / Q000138:chemical synthesis*; D020366:Methylmethacrylate / Q000737:chemistry; D012492:Salts / Q000737:chemistry*; D012996:Solutions; D014867:Water / Q000737:chemistry*
chemical_list: C412621:1-butyl-3-methylimidazolium hexafluorophosphate; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D000441:Hexanols; D007093:Imidazoles; D007477:Ions; D008689:Methacrylates; D012492:Salts; D012996:Solutions; D014867:Water; D020366:Methylmethacrylate; D008049:Lipase; C431349:lipase B, Candida antarctica