title: Disaccharide analysis of glycosaminoglycans synthesized by cardiac myxoma cells in tumor tissues and in cell culture.
abstract: We analyzed the main disaccharide units of glycosaminoglycans synthesized by cardiac myxoma cells in vivo and in cell culture using high-performance liquid chromatography after 1-phenyl-3-methyl-5-pyrasolone labeling. Cardiac myxoma tissues contained large amounts of chondroitin-6-sulfate (46%) and hyaluronic acid (32%), along with some chondroitin-4-sulfate (13%), chondroitin (6%), and much less dermatan sulfate (3%). Cultured cardiac myxoma cells synthesized mainly chondroitin-6-sulfate. The abundant glycosaminoglycans in myxoma tissues may make up the characteristic friable gelatinous matrix which is favorable for embolism and tumor cell growth.
authors.affiliation: Second Department of Internal Medicine, 3-39-22, Showa-machi, Maebashi, Gunma 371-8511, Japan.
authors.forename: Mitsuko
authors.initials: M
authors.lastname: Negishi
authors.forename: Hironosuke
authors.initials: H
authors.lastname: Sakamoto
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Sakamaki
authors.forename: Osamu
authors.initials: O
authors.lastname: Ishikawa
authors.forename: Tsugiyasu
authors.initials: T
authors.lastname: Kanda
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Tamura
authors.forename: Masahiko
authors.initials: M
authors.lastname: Kurabayashi
authors.forename: Ryozo
authors.initials: R
authors.lastname: Nagai
mesh_terms: D000328:Adult; D000368:Aged; D002809:Chondroitin Sulfates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003871:Dermatan Sulfate / Q000032:analysis*; D004187:Disaccharides / Q000032:analysis; D005260:Female; D006338:Heart Neoplasms / Q000737:chemistry* / Q000473:pathology; D006801:Humans; D006820:Hyaluronic Acid / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D009232:Myxoma / Q000737:chemistry* / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000378:metabolism
chemical_list: D004187:Disaccharides; D003871:Dermatan Sulfate; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates