title: Affinity proteomics for systematic protein profiling of chromosome 21 gene products in human tissues.
abstract: Here we show that an affinity proteomics strategy using affinity-purified antibodies raised against recombinant human protein fragments can be used for chromosome-wide protein profiling. The approach is based on affinity reagents raised toward bioinformatics-designed protein epitope signature tags corresponding to unique regions of individual gene loci. The genes of human chromosome 21 identified by the genome efforts were investigated, and the success rates for de novo cloning, protein production, and antibody generation were 85, 76, and 56%, respectively. Using human tissue arrays, a systematic profiling of protein expression and subcellular localization was undertaken for the putative gene products. The results suggest that this affinity proteomics strategy can be used to produce a proteome atlas, describing distribution and expression of proteins in normal tissues as well as in common cancers and other forms of diseased tissues.
authors.affiliation: Department of Biotechnology, AlbaNova University Center, Royal Institute of Technology, Roslagsvägen 30 B, 106 91 Stockholm, Sweden.
authors.forename: Charlotta
authors.initials: C
authors.lastname: Agaton
authors.forename: Joakim
authors.initials: J
authors.lastname: Galli
authors.forename: Ingmarie
authors.initials: I
authors.lastname: Höidén Guthenberg
authors.forename: Lars
authors.initials: L
authors.lastname: Janzon
authors.forename: Marianne
authors.initials: M
authors.lastname: Hansson
authors.forename: Anna
authors.initials: A
authors.lastname: Asplund
authors.forename: Eva
authors.initials: E
authors.lastname: Brundell
authors.forename: Susanne
authors.initials: S
authors.lastname: Lindberg
authors.forename: Irene
authors.initials: I
authors.lastname: Ruthberg
authors.forename: Kenneth
authors.initials: K
authors.lastname: Wester
authors.forename: Dorothee
authors.initials: D
authors.lastname: Wurtz
authors.forename: Christer
authors.initials: C
authors.lastname: Höög
authors.forename: Joakim
authors.initials: J
authors.lastname: Lundeberg
authors.forename: Stefan
authors.initials: S
authors.lastname: Ståhl
authors.forename: Fredrik
authors.initials: F
authors.lastname: Pontén
authors.forename: Mathias
authors.initials: M
authors.lastname: Uhlén
mesh_terms: D000906:Antibodies; D002891:Chromosomes, Human, Pair 21 / Q000378:metabolism*; D019295:Computational Biology; D020224:Expressed Sequence Tags; D006801:Humans; D009928:Organ Specificity; D010865:Pilot Projects; D020543:Proteome / Q000378:metabolism*; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism; D046888:Tissue Array Analysis
chemical_list: D000906:Antibodies; D020543:Proteome; D011994:Recombinant Proteins