[Effect of apoptosis induced by different vitamin E homologous analogues in human hepatoma cells (HepG2)].
To observe the effect of alpha-tocopherol(gamma-T), delta-tocopherol(delta-T), delta-tocopherol(delta-T), and vitamin E succinate (VES) on apoptosis induced in human hepatoma cells (HepG2), the cell apoptosis was detected by flow cytometry (FCM) and DNA ladder under the treatment of different VE homologues analogues (alpha-T, gamma-T, delta-T and VES) at different concentrations (12.5, 25, 50, 100 and 200 mg/L) for 48 hours. The results showed that the growth of HepG2 cells was inhibited by delta-T and VES at 12.5-200 mg/L in comparison with the negative control group, while gamma-T showed weak effect of inhibition and alpha-T did not show any inhibition effect. With the exception of alpha-tocopherol, delta-tocopherol and VES were effective in induction of apoptosis in HepG2 cells at concentrations of 12.5-200 mg/L. gamma-tocopherol showed effect only at 200 mg/L. delta-tocopherol and VES decreased HepG2 cells growth and viability, and increased apoptotic propensity significantly. A dose-dependent of antiproliferation and induction of apoptosis were found in HepG2 cells line. The order of efficiency of four vitamin E analogues was delta-tocopherol > VES > gamma-tocopherol > alpha-tocopherol. It is suggested that delta-tocopherol and VES may be a agent that has a anti-hepatomatic potential.
Min
Junxia
J
Second Military Medical University, Shanghai 200433, China.
Guo
Junsheng
J
Zhao
Faji
F
Cai
Donglian
D
D000970:Antineoplastic Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D024505:Tocopherols; D014407:Tumor Cells, Cultured; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000494:pharmacology*; D024502:alpha-Tocopherol / Q000494:pharmacology; D024504:gamma-Tocopherol / Q000494:pharmacology*
D000970:Antineoplastic Agents; D000975:Antioxidants; D014810:Vitamin E; D024504:gamma-Tocopherol; D024502:alpha-Tocopherol; D024505:Tocopherols