title: Marinomonas primoryensis sp. nov., a novel psychrophile isolated from coastal sea-ice in the Sea of Japan.
abstract: Two bacterial strains (KMM 3633(T) and KMM 3634) were isolated from marine coastal sea-ice and characterized by using phenotypic and molecular methods. The isolates were aerobic, Gram-negative, psychrophilic, halophilic and motile by means of a single polar flagellum. The DNA G + C content was 45.3-45.6 mol%. The major cellular fatty acids were C(16 :0), C(16 : 1)omega9c and C(18 : 1)omega7c. Comparison of almost-complete 16S rDNA sequences demonstrated that the strains were phylogenetically closely related to each other (99.5% sequence identity), and related to Marinomonas species (94.4-96.4% identity). DNA-DNA reassociation between KMM 3633(T) and KMM 3634 occurred at a level of 92%. Based on phenotypic, chemotaxonomic and phylogenetic properties, the name Marinomonas primoryensis sp. nov. is proposed for strains KMM 3633(T) and KMM 3634; the type strain is KMM 3633(T) (= JCM 11775(T) = NRIC 523(T)).
authors.affiliation: Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch, Russian Academy of Sciences, Vladivostok 690022, Prospect 100 Let Vladivostoku 159, Russia.
authors.forename: Lyudmila A
authors.initials: LA
authors.lastname: Romanenko
authors.affiliation: Tokyo University of Agriculture, Department of Applied Biology and Chemistry, Faculty of Applied Bioscience, Laboratory of Food Science and Technology, Sakuragaoka 1-1-1, Setagaya-ku, Tokyo 156-8502, Japan.
authors.forename: Masataka
authors.initials: M
authors.lastname: Uchino
authors.affiliation: Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch, Russian Academy of Sciences, Vladivostok 690022, Prospect 100 Let Vladivostoku 159, Russia.
authors.forename: Valery V
authors.initials: VV
authors.lastname: Mikhailov
authors.affiliation: Institute of Marine Biology, Far-Eastern Branch, Russian Academy of Sciences, Vladivostok 690041, Russia.
authors.forename: Natalia V
authors.initials: NV
authors.lastname: Zhukova
authors.affiliation: Tokyo University of Agriculture, Department of Applied Biology and Chemistry, Faculty of Applied Bioscience, Laboratory of Food Science and Technology, Sakuragaoka 1-1-1, Setagaya-ku, Tokyo 156-8502, Japan.
authors.forename: Tai
authors.initials: T
authors.lastname: Uchimura
mesh_terms: D015373:Bacterial Typing Techniques; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020563:Gammaproteobacteria / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D005838:Genotype; D007053:Ice; D007564:Japan; D008969:Molecular Sequence Data; D009792:Oceans and Seas; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D004275:DNA, Ribosomal; D005227:Fatty Acids; D007053:Ice; D012336:RNA, Ribosomal, 16S