Cell-mediated fas-based lysis of dendritic cells which are apparently resistant to anti-Fas antibody.
In this report, the controversy concerning the sensitivity of dendritic cells (DCs) to Fas-dependent induction of apoptosis was examined using murine DCs. Although DCs could not be lysed when exposed to an anti-Fas antibody, Jo2, the observed resistance turned out to reflect their lack of the expression of Fc(gamma)R necessary for crosslinking the antibody, rather than their intrinsic resistance. Thus, at least a fraction of DCs was sensitive to Jo2 in the presence of Fc(gamma)R-expressing by-standers. Consistently, a significant fraction of DCs was sensitive to Fas-dependent lysis mediated by T cells including the antigen-specific killing by CD4+ T cells. Both immature (class II MHClow) and mature (class II MHChigh) DCs were sensitive to the Fas-based induction of apoptosis.
Yokota
Aya
A
Department of Immunology, Kitasato University School of Medicine, Sagamihara, Kanagawa 228-8555, Japan.
Oikawa
Atsuhiko
A
Matsuda
Chieko
C
Shinohara
Nobukata
N
Eshima
Koji
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000633:toxicity*; D058846:Antibodies, Monoclonal, Murine-Derived; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000166:cytology; D015704:CD4 Antigens / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D004351:Drug Resistance; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D017452:Receptors, IgG / Q000378:metabolism; D013154:Spleen / Q000166:cytology; D019014:fas Receptor / Q000276:immunology / Q000378:metabolism* / Q000502:physiology
D000911:Antibodies, Monoclonal; D058846:Antibodies, Monoclonal, Murine-Derived; D015704:CD4 Antigens; D017452:Receptors, IgG; C411023:anti-Fas monoclonal antibody; D019014:fas Receptor; D007371:Interferon-gamma