Cytological evidence for preservation of mitochondrial and plastid DNA in the mature generative cells of Chlorophytum spp. (Liliaceae).
Following 4',6-diamidino-2-phenylindole staining of mature pollen grains of Chlorophytum comosum, fluorescence microscopy confirmed that cytoplasmic nucleoids (DNA aggregates) were present in the generative cells, which indicated the possibility of biparental cytoplasmic inheritance. Electron and immuno-electron microscopy showed that both plastids and mitochondria were present in the generative cells, and both organelles contained DNA. These results indicate that mitochondria and plastids of C. comosum have the potential for biparental inheritance. Similar results were obtained with mature pollen grains of C. chinense. Therefore, we conclude the coincident biparental inheritance for mitochondria and plastids in the members of the genus Chlorophytum.
Zhang
Q
Q
College of Life Science, Peking University, Beijing, People's Republic of China.
Sodmergen
D004272:DNA, Mitochondrial / Q000032:analysis*; D018744:DNA, Plant / Q000032:analysis*; D019668:Liliaceae / Q000235:genetics* / Q000648:ultrastructure*; D008856:Microscopy, Fluorescence; D016253:Microscopy, Immunoelectron; D018087:Plastids / Q000235:genetics*
D004272:DNA, Mitochondrial; D018744:DNA, Plant