Simultaneous determination of six HIV nucleoside analogue reverse transcriptase inhibitors and nevirapine by liquid chromatography with ultraviolet absorbance detection.
An accurate, sensitive and specific reversed-phase high-performance liquid chromatography assay for the simultaneous quantitative determination of the nucleoside reverse transcriptase inhibitors zalcitabine, lamivudine, didanosine, stavudine, zidovudine, and abacavir with the non-nucleoside reverse transcriptase inhibitor nevirapine in human blood plasma is described. The new Polarity dC C(18) silica column used in this method provides better resolution and peak shape than all other columns tested. Also, four different ultraviolet wavelengths were used for accurate and specific quantitation of the analytes. The method was validated over the range of 10-10000 ng/ml for all analytes except zalcitabine (10-5000 ng/ml). This method is accurate (average accuracies of three different concentrations ranged from 97.2 to 105%), and precise (within- and between-day precision measures ranged from 0.5 to 5.1% and 0.5 to 5.6%, respectively), and is currently being used for determination of plasma drug concentrations in our laboratory.
Rezk
Naser L
NL
Clinical Pharmacology/Analytical Chemistry Core, Center for AIDS Research, 3323A Kerr Hall, CB# 7360, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. naser2@med.unc.edu
Tidwell
Richard R
RR
Kashuba
Angela D M
AD
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D019829:Nevirapine / Q000097:blood*; D018894:Reverse Transcriptase Inhibitors / Q000097:blood*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D018894:Reverse Transcriptase Inhibitors; D019829:Nevirapine