Consistency checks for characterizing protein forms.
Proteomics enforces the reverse chronological order on the gene to protein dogma and imposes amino acid sequences as a starting point of an investigation relative to function. By this approach, proteomics data can confirm the presence of multiple forms of a protein. Notwithstanding variations attributed specific individual features of organisms and tissues, from two to over ten protein forms can be identified in a given sample. The present work describes some guidelines for tracking the origin of alternative protein forms and attempts to tag the details of sequence data in the literature. Working via these guidelines we have uncovered a third alternative form of the Pim subfamily of oncogenes. The term form is here combined with the qualification alternative to describe any product of a given gene including closely related paralogs. This paper also emphasizes the need for consistency checks in annotation processes, such as gene clustering, to avoid losing important details describing protein alternative forms. By identifying alternative protein forms, we illustrate the fact that rationalizing of protein function via the identification of protein-protein interactions should in reality be that of identifying (alternative) form-form interactions.
Chichester
Christine
C
Geneva Bioinformatics, 25 avenue de Champel, Switzerland.
Nikitin
Frederic
F
Ravarini
Jean-Christophe
JC
Lisacek
Frederique
F
D000595:Amino Acid Sequence / Q000235:genetics; D000818:Animals; D019295:Computational Biology / Q000379:methods / Q000592:standards; D018076:DNA, Complementary / Q000145:classification / Q000235:genetics; D030562:Databases, Protein / Q000706:statistics & numerical data; D020224:Expressed Sequence Tags; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000145:classification / Q000235:genetics; D040901:Proteomics / Q000379:methods / Q000592:standards*; D011518:Proto-Oncogene Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D051573:Proto-Oncogene Proteins c-pim-1; D011786:Quality Control; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013552:Swine
D018076:DNA, Complementary; C490587:PIM2 protein, human; D011518:Proto-Oncogene Proteins; C496634:PIM1 protein, human; D017346:Protein Serine-Threonine Kinases; D051573:Proto-Oncogene Proteins c-pim-1; C406031:proto-oncogene proteins pim