Retrovirally mediated overexpression of peroxiredoxin VI increases the survival of WI-38 human diploid fibroblasts exposed to cytotoxic doses of tert-butylhydroperoxide and UVB.
In this work, stable overexpression of peroxiredoxin VI was generated in WI-38 human diploid fibroblasts using a retrovirus-mediated transfection system. Estimation of cell survival showed that peroxiredoxin VI provides a significant protection against tert-butylhydroperoxide- or UVB-caused cytotoxicity. No protection was found against ethanol- or H(2)O(2)-caused cytotoxicity. These effects are correlated with the known functions of Prx VI.
Dierick
Jean-François
JF
Unit of Research on Cellular Biology, University of Namur (FUNDP), Rue de Bruxelles 61, B-5000 Namur, Belgium.
Wenders
Frédéric
F
Chainiaux
Florence
F
Remacle
José
J
Fisher
Aron B
AB
Toussaint
Olivier
O
D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects; D004171:Diploidy; D000431:Ethanol / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic; D015967:Gene Expression Regulation, Viral; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects; D010544:Peroxidases / Q000235:genetics*; D054465:Peroxiredoxin VI; D054464:Peroxiredoxins; D012190:Retroviridae / Q000235:genetics*; D012997:Solvents / Q000494:pharmacology; D014162:Transfection; D014466:Ultraviolet Rays; D020122:tert-Butylhydroperoxide / Q000494:pharmacology*
D016877:Oxidants; D012997:Solvents; D000431:Ethanol; D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide; D010544:Peroxidases; D054465:Peroxiredoxin VI; D054464:Peroxiredoxins