title: Identification and characterization of microsatellite markers in the Chagas disease vector Triatoma dimidiata.
abstract: Triatoma dimidiata, one of the major vectors of Chagas disease in Central America, is found in both domestic and peri-domestic habitats. Questions concerning population boundaries, infestation rates, insecticide resistance, and geographic dispersal of triatomine bugs persist and may be resolved using genetic markers such as microsatellites. Microsatellites are short tandem repeats found dispersed throughout a genome and can be useful for genotypic identification. We developed a plasmid library from the genomic DNA isolated from a single T. dimidiata adult collected in Guatemala. Ten thousand clones were screened using a probe consisting of nine microsatellite oligonucleotides. Eight loci appear polymorphic among populations found in Guatemala, Honduras, and Mexico, and thus are potentially useful for population genetic applications.
authors.affiliation: Centers for Disease Control and Prevention, Division of Parasitic Diseases, National Center for Infectious Diseases, Entomology Branch, Atlanta, GA 30341, USA.
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Anderson
authors.forename: James E
authors.initials: JE
authors.lastname: Lai
authors.forename: Ellen M
authors.initials: EM
authors.lastname: Dotson
authors.forename: Celia
authors.initials: C
authors.lastname: Cordon-Rosales
authors.forename: Carlos
authors.initials: C
authors.lastname: Ponce
authors.forename: Douglas E
authors.initials: DE
authors.lastname: Norris
authors.forename: C Ben
authors.initials: CB
authors.lastname: Beard
mesh_terms: D000818:Animals; D014355:Chagas Disease / Q000635:transmission; D005828:Genetics, Population; D007303:Insect Vectors / Q000235:genetics*; D018895:Microsatellite Repeats; D014224:Triatoma / Q000235:genetics*