title: The Upf-dependent decay of wild-type PPR1 mRNA depends on its 5'-UTR and first 92 ORF nucleotides.
abstract: mRNAs containing premature translation termination codons (nonsense mRNAs) are targeted for deadenylation-independent degradation in a mechanism that depends on Upf1p, Upf2p and Upf3p. This decay pathway is often called nonsense- mediated mRNA decay (NMD). Nonsense mRNAs are decapped by Dcp1p and then degraded 5' to 3' by Xrn1p. In the yeast Saccharomyces cerevisiae, a significant number of wild-type mRNAs accumulate in upf mutants. Wild-type PPR1 mRNA is one of these mRNAs. Here we show that PPR1 mRNA degradation depends on the Upf proteins, Dcp1p, Xrn1p and Hrp1p. We have mapped an Upf1p-dependent destabilizing element to a region located within the 5'-UTR and the first 92 bases of the PPR1 ORF. This element targets PPR1 mRNA for Upf-dependent decay by a novel mechanism.
authors.affiliation: School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE 68588-0666, USA.
authors.forename: B
authors.initials: B
authors.lastname: Kebaara
authors.forename: T
authors.initials: T
authors.lastname: Nazarenus
authors.forename: R
authors.initials: R
authors.lastname: Taylor
authors.forename: A
authors.initials: A
authors.lastname: Forch
authors.forename: A L
authors.initials: AL
authors.lastname: Atkin
mesh_terms: D020121:5' Untranslated Regions; D048868:Adaptor Proteins, Signal Transducing; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004722:Endoribonucleases / Q000235:genetics / Q000502:physiology; D005095:Exoribonucleases / Q000235:genetics / Q000502:physiology; D016366:Open Reading Frames; D039381:RNA Cap-Binding Proteins; D020365:RNA Helicases / Q000502:physiology; D020871:RNA Stability; D012331:RNA, Fungal / Q000737:chemistry / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry* / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D038621:Regulatory Sequences, Ribonucleic Acid; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000502:physiology*; D017384:Sequence Deletion; D015534:Trans-Activators / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D039221:mRNA Cleavage and Polyadenylation Factors / Q000502:physiology
chemical_list: D020121:5' Untranslated Regions; D048868:Adaptor Proteins, Signal Transducing; D004268:DNA-Binding Proteins; C092470:NMD2 protein, S cerevisiae; C065682:PPR1 protein, S cerevisiae; D039381:RNA Cap-Binding Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D038621:Regulatory Sequences, Ribonucleic Acid; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C096360:UPF3 protein, S cerevisiae; D039221:mRNA Cleavage and Polyadenylation Factors; C100868:DCP1 protein, S cerevisiae; D004722:Endoribonucleases; D005095:Exoribonucleases; C068398:XRN1 protein, S cerevisiae; C074151:NAM7 protein, S cerevisiae; D020365:RNA Helicases