Screening and selection of hypoallergenic buckwheat species.
Both common buckwheat (Fagopyrum esculentum) flour and meal cause an allergy in sensitive patients, and if unnoticed, it can be fatal. It has become a potential occupational hazard for some mill workers. The development of hypoallergenic buckwheat would be more efficient if natural mutants for allergenic protein are detected. A screening and selection method was developed using SDS-PAGE coupled with PCR techniques. SDS-PAGE analysis of 14 different species of buckwheat revealed that F. lineare and F. urophyllum lack the 22-kDa major allergenic protein. PCR-based screening with specific primers for sequences encoding the allergenic protein was also effective in distinguishing the allergen-deficient species.
Nair
Arun
A
Kiyomoto Bio Co. Ltd., 2-28-4 Sakura, Gakuen Kibanadai, Miyazaki 889-2154, Japan. nairarunc@yahoo.com
Adachi
Taiji
T
D000485:Allergens / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D019612:Fagopyrum / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D009154:Mutation; D010940:Plant Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment
D000485:Allergens; D010940:Plant Proteins