title: Effective small interfering RNAs and phosphorothioate antisense DNAs have different preferences for target sites in the luciferase mRNAs.
abstract: Antisense DNA target sites can be selected by the accessibility of the mRNA target. It remains unknown whether a mRNA site that is accessible to an antisense DNA is also a good candidate target site for a siRNA. Here, we reported a parallel analysis of 12 pairs of antisense DNAs and siRNA duplexes for their potency to inhibit reporter luciferase activity in mammalian cells, both of the antisense DNA and siRNA agents in a pair being directed to same site in the mRNA. Five siRNAs and two antisense DNAs turned out to be effective, but the sites targeted by those effective siRNAs and antisense DNAs did not overlap. Our results indicated that effective antisense DNAs and siRNAs have different preferences for target sites in the mRNA.
authors.affiliation: Center for Genomics and Bioinformatics, Karolinska Institute, 17177, Stockholm, Sweden.
authors.forename: Yunhe
authors.initials: Y
authors.lastname: Xu
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Zhang
authors.forename: Dorit
authors.initials: D
authors.lastname: Thormeyer
authors.forename: Ola
authors.initials: O
authors.lastname: Larsson
authors.forename: Quan
authors.initials: Q
authors.lastname: Du
authors.forename: Joacim
authors.initials: J
authors.lastname: Elmén
authors.forename: Claes
authors.initials: C
authors.lastname: Wahlestedt
authors.forename: Zicai
authors.initials: Z
authors.lastname: Liang
mesh_terms: D000818:Animals; D002460:Cell Line; D016373:DNA, Antisense; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D009690:Nucleic Acid Conformation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering
chemical_list: D016373:DNA, Antisense; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D008156:Luciferases