title: Structural and functional properties of IL-4delta2, an alternative splice variant of human IL-4.
abstract: Structural and functional properties of recombinant IL-4delta2, a naturally occurring splice variant of human IL-4 with a deletion of the loop region 22-37, have been analyzed. IL-4delta2 has alpha-helical structure and most likely preserves the "up-up-down-down" topology typical of the four-helix-bundle cytokines. IL-4delta2 interacts specifically with the alpha chain of IL-4R and competes effectively with IL-4 for the common binding sites. Thus, IL-4delta2 may act as a regulator of the cytokine net, being the natural antagonist of IL-4.
authors.affiliation: Institute of Immunological Engineering, 142380 Lyubuchany, Moscow Region, Russia.
authors.forename: Anatoly M
authors.initials: AM
authors.lastname: Vasiliev
authors.forename: Raisa N
authors.initials: RN
authors.lastname: Vasilenko
authors.forename: Nataly L
authors.initials: NL
authors.lastname: Kulikova
authors.forename: Sergey M
authors.initials: SM
authors.lastname: Andreev
authors.forename: Irina O
authors.initials: IO
authors.lastname: Chikileva
authors.forename: Galina Yu
authors.initials: GY
authors.lastname: Puchkova
authors.forename: Igor V
authors.initials: IV
authors.lastname: Kosarev
authors.forename: Anna V
authors.initials: AV
authors.lastname: Khodyakova
authors.forename: Valentin S
authors.initials: VS
authors.lastname: Khlebnikov
authors.forename: Leonid R
authors.initials: LR
authors.lastname: Ptitsyn
authors.forename: Grygory Ya
authors.initials: GY
authors.lastname: Shcherbakov
authors.forename: Vladimir N
authors.initials: VN
authors.lastname: Uversky
authors.forename: Lawrence M
authors.initials: LM
authors.lastname: DuBuske
authors.forename: Vyacheslav M
authors.initials: VM
authors.lastname: Abramov
mesh_terms: D017398:Alternative Splicing; D002455:Cell Division / Q000502:physiology; D002942:Circular Dichroism; D003001:Cloning, Molecular; D003553:Cystine / Q000378:metabolism; D015847:Interleukin-4 / Q000235:genetics* / Q000378:metabolism; D008024:Ligands; D020033:Protein Isoforms / Q000235:genetics* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D017550:Spectroscopy, Fourier Transform Infrared; D013950:Thymus Gland / Q000378:metabolism
chemical_list: D008024:Ligands; D020033:Protein Isoforms; D015847:Interleukin-4; D003553:Cystine