Applications of one-bead one-compound combinatorial libraries and chemical microarrays in signal transduction research.
The "one-bead-one-compound" (OBOC) combinatorial library method synthesizes millions of random compounds such that each bead displays only one compound. Bead libraries are screened, and positive beads are isolated for structure analysis. Peptide substrates and inhibitors of protein kinases, and peptide ligands for cell surface receptors have been identified using this method. A novel encoding strategy for OBOC libraries has been developed to identify peptidomimetic and small-molecule ligands that specifically interact with cellular proteins. These ligands will be tested for their effects on cell signaling and used to construct chemical microarrays for further characterization of ligand-protein interactions.
Lam
Kit S
KS
UC Davis Cancer Center, Division of Hematology/Oncology, and Department of Internal Medicine, University of California-Davis, 4501 X Street, Sacramento, California 95817, USA. kit.lam@ucdmc.ucdavis.edu
Liu
Ruiwu
R
Miyamoto
Suzanne
S
Lehman
Alan L
AL
Tuscano
Joseph M
JM
D020650:Combinatorial Chemistry Techniques; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D008565:Membrane Proteins / Q000378:metabolism; D019151:Peptide Library; D040081:Protein Array Analysis / Q000379:methods*; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000378:metabolism; D015398:Signal Transduction; D013379:Substrate Specificity
D004791:Enzyme Inhibitors; D008565:Membrane Proteins; D019151:Peptide Library; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases