Viability of basidiomycete strains after cryopreservation: comparison of two different freezing protocols.
The viability of 250 basidiomycete strains was determined after a 2-d and then after a 2-year storage under liquid nitrogen using two different freezing protocols. Using an original agar plug protocol (OP), 162 strains (65%) of the 250 strains survived a 2-d storage and 158 strains (63%) survived a 2-year storage in liquid nitrogen. Using a straw protocol (CP), 246 strains (98%) of the 250 strains survived a 2-d storage and 243 strains (97%) a 2-year storage in liquid nitrogen. In addition, other 106 strains were newly estimated using the CP protocol; 104 (98%) of them survived successfully a 2-d storage and 101 (95%) of them survived a 2-year storage in liquid nitrogen. The results indicate that the protocol used for cryopreservation can significantly influence strain survival. Markedly better results were obtained using the CP protocol.
Homolka
L
L
Institute of Microbiology, Academy of Sciences of the Czech Republic, 142 20 Prague, Czechia. homolka@biomed.cas.cz
Lisá
L
L
Nerud
F
F
D001487:Basidiomycota / Q000145:classification / Q000201:enzymology / Q000254:growth & development* / Q000502:physiology; D015925:Cryopreservation / Q000379:methods*; D003470:Culture Media; D042845:Laccase; D009172:Mycology / Q000379:methods; D010088:Oxidoreductases / Q000378:metabolism
D003470:Culture Media; D010088:Oxidoreductases; D042845:Laccase