Purification and characterization of perchloric acid soluble protein from rat lung.
We isolated a perchloric acid soluble protein from the post-mitochondria supernatant fraction of the rat lung and designated it as RLu-PSP1. The protein is soluble in 5% perchloric acid and was purified by ammonium sulfate fractionation and CM-Sephadex chromatography. The amino acid sequence of RLu-PSP was identical with that of rat liver PSP (RL-PSP). RLu-PSP inhibited protein synthesis in a rabbit reticulocyte lysate system. It was expressed mainly in cytoplasm of bronchioles and alveolar epithelial cells of the lung from 60-day-old rats. In 15-day-old rat embryos, the epithelial-lining of the terminal buds of the respiratory tree was immunopositive. The expression of RLu-PSP increased from the embryonic 15th day to the postnatal 40th day. This is the first report on the presence of a PSP in rat lung and on its involvement in the regulation of cellular growth and differentiation.
Matsumoto
M
M
Department of Veterinary Medicine, Faculty of Agriculture, Kagoshima University, Kagoshima 890-0065, Japan.
Kanouchi
H
H
Suzuki
K
K
Kaneki
K
K
Kawasaki
Y
Y
Oka
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004622:Embryo, Mammalian / Q000737:chemistry; D006360:Heat-Shock Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D008168:Lung / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D011817:Rabbits; D051381:Rats; D011913:Rats, Inbred BB; D012260:Ribonucleases / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012697:Serine Endopeptidases / Q000378:metabolism
D006360:Heat-Shock Proteins; C097211:Rida protein, rat; D012260:Ribonucleases; D012697:Serine Endopeptidases; C036813:lysyl endopeptidase