title: Improved serodiagnosis of erythema migrans using novel recombinant borrelial BBK32 antigens.
abstract: The performances of recombinant borrelial BBK32 proteins as antigens in the serology of erythema migrans (EM) were evaluated in an ELISA. Serum samples were obtained from 75 patients from different geographic areas where three borrelial species, Borrelia burgdorferi sensu stricto, Borrelia afzelii or Borrelia garinii, cause Lyme borreliosis. Antibodies to variant BBK32 proteins were compared with anti-flagella or with anti-IR(6) peptide antibodies. In IgG ELISA at presentation of EM, 65/75 (87 %) patients had antibodies to one or more variants of BBK32, 29/75 (39 %) had antibodies to flagella and 29/75 (39 %) had antibodies to the VlsE IR(6) peptide antigen. The immunoreactivity against variant BBK32 proteins differed in patients from different geographic regions. The present results suggest that the BBK32 proteins used in combination or in parallel may improve the laboratory diagnosis of EM.
authors.affiliation: Hospital for Children and Adolescents, University of Helsinki, Stenbäckinkatu 11, 00290 Helsinki, Finland 2Department of Bacteriology and Immunology, Haartman Institute, Haartmaninkatu 3, 00014 Helsinki, Finland 3,7Department of Otolaryngology3 and Laboratory Diagnostics7, Helsinki University Central Hospital, 00290 Helsinki, Finland 4Division of Rheumatology, Allergy and Immunology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA 5Department of Infectious Diseases, University Medical Center Ljubljana, Slovenia 6Professor-Hess-Children's Hospital Bremen and Children's Hospital of the University of Würzburg, Germany.
authors.forename: Pekka
authors.initials: P
authors.lastname: Lahdenne
authors.affiliation: Hospital for Children and Adolescents, University of Helsinki, Stenbäckinkatu 11, 00290 Helsinki, Finland 2Department of Bacteriology and Immunology, Haartman Institute, Haartmaninkatu 3, 00014 Helsinki, Finland 3,7Department of Otolaryngology3 and Laboratory Diagnostics7, Helsinki University Central Hospital, 00290 Helsinki, Finland 4Division of Rheumatology, Allergy and Immunology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA 5Department of Infectious Diseases, University Medical Center Ljubljana, Slovenia 6Professor-Hess-Children's Hospital Bremen and Children's Hospital of the University of Würzburg, Germany.
authors.forename: Jaana
authors.initials: J
authors.lastname: Panelius
authors.affiliation: Hospital for Children and Adolescents, University of Helsinki, Stenbäckinkatu 11, 00290 Helsinki, Finland 2Department of Bacteriology and Immunology, Haartman Institute, Haartmaninkatu 3, 00014 Helsinki, Finland 3,7Department of Otolaryngology3 and Laboratory Diagnostics7, Helsinki University Central Hospital, 00290 Helsinki, Finland 4Division of Rheumatology, Allergy and Immunology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA 5Department of Infectious Diseases, University Medical Center Ljubljana, Slovenia 6Professor-Hess-Children's Hospital Bremen and Children's Hospital of the University of Würzburg, Germany.
authors.forename: Harri
authors.initials: H
authors.lastname: Saxen
authors.affiliation: Hospital for Children and Adolescents, University of Helsinki, Stenbäckinkatu 11, 00290 Helsinki, Finland 2Department of Bacteriology and Immunology, Haartman Institute, Haartmaninkatu 3, 00014 Helsinki, Finland 3,7Department of Otolaryngology3 and Laboratory Diagnostics7, Helsinki University Central Hospital, 00290 Helsinki, Finland 4Division of Rheumatology, Allergy and Immunology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA 5Department of Infectious Diseases, University Medical Center Ljubljana, Slovenia 6Professor-Hess-Children's Hospital Bremen and Children's Hospital of the University of Würzburg, Germany.
authors.forename: Tero
authors.initials: T
authors.lastname: Heikkilä
authors.affiliation: Hospital for Children and Adolescents, University of Helsinki, Stenbäckinkatu 11, 00290 Helsinki, Finland 2Department of Bacteriology and Immunology, Haartman Institute, Haartmaninkatu 3, 00014 Helsinki, Finland 3,7Department of Otolaryngology3 and Laboratory Diagnostics7, Helsinki University Central Hospital, 00290 Helsinki, Finland 4Division of Rheumatology, Allergy and Immunology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA 5Department of Infectious Diseases, University Medical Center Ljubljana, Slovenia 6Professor-Hess-Children's Hospital Bremen and Children's Hospital of the University of Würzburg, Germany.
authors.forename: Heidi
authors.initials: H
authors.lastname: Sillanpää
authors.affiliation: Hospital for Children and Adolescents, University of Helsinki, Stenbäckinkatu 11, 00290 Helsinki, Finland 2Department of Bacteriology and Immunology, Haartman Institute, Haartmaninkatu 3, 00014 Helsinki, Finland 3,7Department of Otolaryngology3 and Laboratory Diagnostics7, Helsinki University Central Hospital, 00290 Helsinki, Finland 4Division of Rheumatology, Allergy and Immunology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA 5Department of Infectious Diseases, University Medical Center Ljubljana, Slovenia 6Professor-Hess-Children's Hospital Bremen and Children's Hospital of the University of Würzburg, Germany.
authors.forename: Miikka
authors.initials: M
authors.lastname: Peltomaa
authors.affiliation: Hospital for Children and Adolescents, University of Helsinki, Stenbäckinkatu 11, 00290 Helsinki, Finland 2Department of Bacteriology and Immunology, Haartman Institute, Haartmaninkatu 3, 00014 Helsinki, Finland 3,7Department of Otolaryngology3 and Laboratory Diagnostics7, Helsinki University Central Hospital, 00290 Helsinki, Finland 4Division of Rheumatology, Allergy and Immunology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA 5Department of Infectious Diseases, University Medical Center Ljubljana, Slovenia 6Professor-Hess-Children's Hospital Bremen and Children's Hospital of the University of Würzburg, Germany.
authors.forename: Maja
authors.initials: M
authors.lastname: Arnez
authors.affiliation: Hospital for Children and Adolescents, University of Helsinki, Stenbäckinkatu 11, 00290 Helsinki, Finland 2Department of Bacteriology and Immunology, Haartman Institute, Haartmaninkatu 3, 00014 Helsinki, Finland 3,7Department of Otolaryngology3 and Laboratory Diagnostics7, Helsinki University Central Hospital, 00290 Helsinki, Finland 4Division of Rheumatology, Allergy and Immunology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA 5Department of Infectious Diseases, University Medical Center Ljubljana, Slovenia 6Professor-Hess-Children's Hospital Bremen and Children's Hospital of the University of Würzburg, Germany.
authors.forename: Hans-Iko
authors.initials: HI
authors.lastname: Huppertz
authors.affiliation: Hospital for Children and Adolescents, University of Helsinki, Stenbäckinkatu 11, 00290 Helsinki, Finland 2Department of Bacteriology and Immunology, Haartman Institute, Haartmaninkatu 3, 00014 Helsinki, Finland 3,7Department of Otolaryngology3 and Laboratory Diagnostics7, Helsinki University Central Hospital, 00290 Helsinki, Finland 4Division of Rheumatology, Allergy and Immunology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA 5Department of Infectious Diseases, University Medical Center Ljubljana, Slovenia 6Professor-Hess-Children's Hospital Bremen and Children's Hospital of the University of Würzburg, Germany.
authors.forename: Ilkka J T
authors.initials: IJT
authors.lastname: Seppälä
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D025065:Borrelia burgdorferi / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D015787:Erythema Chronicum Migrans / Q000175:diagnosis* / Q000276:immunology; D005260:Female; D006801:Humans; D008297:Male; D011994:Recombinant Proteins / Q000276:immunology; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D011994:Recombinant Proteins; C106209:p35 antigen, Borrelia