title: Purification of lipid rafts from cultured cells.
abstract: Lipid-rich lipid rafts are microdomains of the plasma membrane that are resistant to low concentrations of nonionic detergent. This forms the basis for their isolation. Either a microsomal fraction or a postnuclear supernatant are loaded beneath a discontinuous iodixanol gradient. If all the solutions contain 0.5-1.0% Triton X-100, the intact lipid rafts float to the top of the gradient while all of the other detergent-solubilized membranes remain at the bottom.
authors.affiliation: School of Biomolecular Sciences, Liverpool John Moores University. john@jgrescon.fsbusiness.co.uk
authors.forename: John M
authors.initials: JM
authors.lastname: Graham
mesh_terms: D002458:Cell Fractionation / Q000379:methods*; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D021962:Membrane Microdomains