Comprehensive identification of human bZIP interactions with coiled-coil arrays.
In eukaryotes, the combinatorial association of sequence-specific DNA binding proteins is essential for transcription. We have used protein arrays to test 492 pairings of a nearly complete set of coiled-coil strands from human basic-region leucine zipper (bZIP) transcription factors. We find considerable partnering selectivity despite the bZIPs' homologous sequences. The interaction data are of high quality, as assessed by their reproducibility, reciprocity, and agreement with previous observations. Biophysical studies in solution support the relative binding strengths observed with the arrays. New associations provide insights into the circadian clock and the unfolded protein response.
Newman
John R S
JR
Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
Keating
Amy E
AE
D000595:Amino Acid Sequence; D050976:Basic-Leucine Zipper Transcription Factors; D002851:Chromatography, High Pressure Liquid; D002940:Circadian Rhythm; D002942:Circular Dichroism; D017362:Cyclic AMP Response Element-Binding Protein / Q000737:chemistry / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D019281:Dimerization; D051790:G-Box Binding Factors; D006801:Humans; D016350:Leucine Zippers; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D040081:Protein Array Analysis; D011485:Protein Binding; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D015398:Signal Transduction; D013696:Temperature; D013816:Thermodynamics; D014157:Transcription Factors / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*
D050976:Basic-Leucine Zipper Transcription Factors; D017362:Cyclic AMP Response Element-Binding Protein; D004268:DNA-Binding Proteins; D051790:G-Box Binding Factors; D010455:Peptides; D014157:Transcription Factors