Use of ceramic monoliths as stationary phase in affinity chromatography.
The use of coated ceramic monoliths as support for affinity chromatography is described. Ceramic monoliths are robust active matrix supports and present a very small pressure drop. Monoliths are coated with a very thin agarose gel layer and activated using a standard activation process for agarose beads. Experiments demonstrate that enzyme adsorption occurs exclusively on the outside surface of the agarose coating since enzyme molecules are too large to fit into the porous matrix. Adsorption and desorption rates are large and production of enzyme per unit monolith volume justifies further exploring this separation process for large throughput operation.
Martin del Valle
Eva
E
Department of Chemical and Materials Engineering, University of Alabama in Huntsville, Huntsville, Alabama 35899, USA.
Galan Serrano
Miguel A
MA
Cerro
Ramon L
RL
D001215:Asparaginase / Q000737:chemistry / Q000302:isolation & purification*; D002516:Ceramics / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000295:instrumentation* / Q000379:methods*; D002847:Chromatography, Agarose / Q000295:instrumentation / Q000379:methods; D045424:Complex Mixtures / Q000302:isolation & purification*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008567:Membranes, Artificial; D044367:Phase Transition; D012685:Sepharose / Q000737:chemistry*; D014357:Trypsin / Q000737:chemistry / Q000302:isolation & purification*
D045424:Complex Mixtures; D008567:Membranes, Artificial; D012685:Sepharose; D014357:Trypsin; D001215:Asparaginase