Expression of vascular endothelial growth factor and its receptors in rat neural stem cells.
Neural stem cells serve neurogenesis in several areas of the adult mammalian brain. The present study investigates an additional feature, i.e. the expression of vascular endothelial growth factor (VEGF) and its receptors in cultured neurospheres from hippocampus, subventricular zone, and olfactory bulb in adult rats. RT-PCR showed that all three lines expressed VEGF mRNA, but different sets of VEGF receptor mRNAs. Stimulation by either 50 ng/ml VEGF-A165 or by 24 h of anoxia resulted in an altered pattern of receptor expression for VEGF-R2 (Flk-1), VEGF-R3 (Flt-4), neuropilin-1, and neuropilin-2, whereas VEGF-R1 (Flt-1) remained unexpressed. The basal expression of VEGF and of several of its receptor mRNAs indicates a hitherto unknown angiogenic potential of neural stem cells.
Maurer
Martin H
MH
Department of Physiology and Pathophysiology, University of Heidelberg, Im Neuenheimer Feld 326, 69120, Heidelberg, Germany. maurer@uni-hd.de
Tripps
Wolf K C
WK
Feldmann
Robert E
RE
Kuschinsky
Wolfgang
W
D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D015687:Cell Hypoxia; D002478:Cells, Cultured; D016228:Endothelial Growth Factors / Q000096:biosynthesis*; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis*; D008222:Lymphokines / Q000096:biosynthesis*; D008297:Male; D009830:Olfactory Bulb / Q000166:cytology / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D040262:Receptors, Vascular Endothelial Growth Factor / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D016228:Endothelial Growth Factors; D036341:Intercellular Signaling Peptides and Proteins; D008222:Lymphokines; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D040262:Receptors, Vascular Endothelial Growth Factor