Synthesis, structural characterization, and immunological properties of carbon nanotubes functionalized with peptides.
Carbon nanotubes (NTs) are becoming highly attractive molecules for applications in medicinal chemistry. The main problem of insolubility in aqueous media has been solved by developing a synthetic protocol that allows highly water-soluble carbon NTs to be obtained. As a result, biologically active peptides can be easily linked through a stable covalent bond to carbon NTs. We have demonstrated that a bound peptide from the foot-and-mouth disease virus, corresponding to the 141-159 region of the viral envelope protein VP1, retained the structural integrity and was recognized by monoclonal and polyclonal antibodies. In addition, this peptide-NT conjugate is immunogenic, eliciting antibody responses of the right specificity. Such a system could be greatly advantageous for diagnostic purposes and could find future applications in vaccine delivery.
Pantarotto
Davide
D
Institute of Molecular and Cellular Biology, UPR 9021 CNRS, 67084 Strasbourg, France.
Partidos
Charalambos D
CD
Graff
Roland
R
Hoebeke
Johan
J
Briand
Jean-Paul
JP
Prato
Maurizio
M
Bianco
Alberto
A
D036022:Capsid Proteins / Q000737:chemistry* / Q000276:immunology*; D002851:Chromatography, High Pressure Liquid; D018985:Epitopes, B-Lymphocyte / Q000737:chemistry / Q000276:immunology; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology; D036103:Nanotechnology / Q000379:methods; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000276:immunology*; D019906:Nuclear Magnetic Resonance, Biomolecular; D010446:Peptide Fragments / Q000737:chemistry* / Q000276:immunology*
D036022:Capsid Proteins; D018985:Epitopes, B-Lymphocyte; D037742:Nanotubes, Carbon; D010446:Peptide Fragments; C442501:VP1 protein, Foot-and-mouth disease virus