Retinoic acid receptor-beta mRNA expression during chemoprevention of hamster cheek pouch carcinogenesis by garlic.
The effect of aqueous garlic (Allium sativum Linn.) on retinoic acid receptor beta (RARbeta) mRNA expression was investigated in male Syrian hamsters during 12-dimethyl enz[a]anthracene (DMBA)-induced hamster buccal pouch (HBP) carcinogenesis. RARbeta mRNA expression was analysed by slot blotted hybridization with radiolabelled RAR-beta probe. In DMBA-induced HBP tumours, decreased expression of RARbeta mRNA was observed. Administration of garlic (250mg/kg body weight) to animals painted with DMBA restored RARbeta mRNA expression to normal pattern suggesting that this may be one of the mechanisms by which garlic exerts its chemopreventive effects.
Balasenthil
Seetharaman
S
Department of Biochemistry, Faculty of Science, Annamalai University, Annamalainagar, Tamil Nadu, India.
Rao
Kunchala S
KS
Nagini
Siddavaram
S
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology / Q000627:therapeutic use*; D002273:Carcinogens; D002610:Cheek; D006224:Cricetinae; D005737:Garlic / Q000737:chemistry*; D008297:Male; D008647:Mesocricetus; D009062:Mouth Neoplasms / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D012333:RNA, Messenger / Q000378:metabolism; D018168:Receptors, Retinoic Acid / Q000235:genetics / Q000378:metabolism*; D016896:Treatment Outcome
D000972:Antineoplastic Agents, Phytogenic; D002273:Carcinogens; D010936:Plant Extracts; D012333:RNA, Messenger; D018168:Receptors, Retinoic Acid; C082925:retinoic acid receptor beta; D015127:9,10-Dimethyl-1,2-benzanthracene