Effect of oral administration of Arabic gum on cisplatin-induced nephrotoxicity in rats.
It has been recently postulated from our laboratory that Arabic gum (AG) offers a protective effect in the kidney of rats against nephrotoxicity induced by gentamicin via inhibiting lipid peroxidation. It has also recently shown a powerful antioxidant effect through scavenging superoxide anions. In this study we utilized a rat model of cisplatin (CP)-induced nephrotoxicity to determine its peak time following (1, 2, 5, and 7 days) of a single CP (7.5 mg/kg, i.p.) injection. Also, a possible protective effect of cotreatment with AG (7.5 g/kg/day p.o.) on CP-induced nephrotoxicity was investigated. Biochemical as well as histological assessments were carried out. CP-induced nephrotoxicity was manifested by significant elevations of the functional parameters blood urea, serum creatinine, and kidney/body weight ratio. Maximum toxic effects of CP were observed 5 days after its injection, while it started after day 1 in the biochemical parameters, such as glutathione depletion in the kidney tissue with concomitant increases in lipid peroxides and platinum content. Additionally, severe necrosis and desquamation of tubular epithelial cells in renal cortex as well as interstitial nephritis were observed after 5 days in CP-treated animals. Five days after AG cotreatment with CP did not protect the kidney from the damaging effects of CP. However, it significantly reduced CP-induced lipid peroxidation. These findings suggest that lipid peroxidation is not the main cause of CP-induced nephrotoxicity but it is rather more dependent on other factors such as platinum disposition in renal interstitial tubules.
Al-Majed
Abdulhakeem A
AA
Department of Pharmacology, College of Pharmacy, King Saud University, Riyadh 11451, Kingdom of Saudi Arabia. majed107@hotmail.com
Abd-Allah
Adel R A
AR
Al-Rikabi
Ammar C
AC
Al-Shabanah
Othman A
OA
Mostafa
Adel M
AM
D000818:Animals; D000970:Antineoplastic Agents / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D001806:Blood Urea Nitrogen; D002945:Cisplatin / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D003404:Creatinine / Q000097:blood; D005079:Excipients / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D006170:Gum Arabic / Q000494:pharmacology*; D007202:Indicators and Reagents; D007668:Kidney / Q000473:pathology; D007674:Kidney Diseases / Q000139:chemically induced* / Q000517:prevention & control*; D007677:Kidney Function Tests; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D000970:Antineoplastic Agents; D005079:Excipients; D007202:Indicators and Reagents; D008054:Lipid Peroxides; D008315:Malondialdehyde; D006170:Gum Arabic; D003404:Creatinine; D005978:Glutathione; D002945:Cisplatin