title: Chronically administered guanosine is anticonvulsant, amnesic and anxiolytic in mice.
abstract: Acute administration of intraperitoneal and oral guanosine has been shown to prevent quinolinic acid and alpha-dendrotoxin-induced seizures in rats and mice. In this study, we investigated the effects of 2 weeks ad libitum consumption of guanosine (0.5 mg/ml) added to mice water supply on seizures and lethality induced by the alpha-dendrotoxin, hole-board behavior, inhibitory avoidance task, locomotor activity, motor coordination, rectal temperature, body weight, and water and food consumption. Guanosine prevented seizures in 40% and death in 50% on mice treated with i.c.v. alpha-dendrotoxin; it also impaired inhibitory avoidance memory and increased head-dipping behavior and locomotor activity on the hole-board test. Guanosine consumption did not alter any of the other parameters evaluated. The anticonvulsant, amnesic, and anxyolytic-like effects may be associated with the ability of guanosine in modulating the glutamatergic excitatory system. Adding to previously reported data, these findings suggest a potential role for chronic guanosine in the management of diseases associated with glutamatergic excitotoxicity, including epilepsy and anxiety.
authors.affiliation: Department of Biochemistry, ICBS, Federal University of Rio Grande do Sul, Avenida Ramiro Barcelos, 2600-Anexo, CEP 90035-003 Porto Alegre, RS, Brazil.
authors.forename: Elsa Regina
authors.initials: ER
authors.lastname: Vinadé
authors.forename: André Prato
authors.initials: AP
authors.lastname: Schmidt
authors.forename: Marcos Emílio Santos
authors.initials: ME
authors.lastname: Frizzo
authors.forename: Ivan
authors.initials: I
authors.lastname: Izquierdo
authors.forename: Elaine
authors.initials: E
authors.lastname: Elisabetsky
authors.forename: Diogo Onofre
authors.initials: DO
authors.lastname: Souza
mesh_terms: D000818:Animals; D014151:Anti-Anxiety Agents / Q000008:administration & dosage / Q000627:therapeutic use; D000927:Anticonvulsants / Q000008:administration & dosage / Q000627:therapeutic use*; D001007:Anxiety / Q000517:prevention & control; D001522:Behavior, Animal / Q000187:drug effects; D001831:Body Temperature / Q000187:drug effects; D004333:Drug Administration Routes; D004334:Drug Administration Schedule; D004546:Elapid Venoms / Q000633:toxicity; D005106:Exploratory Behavior / Q000187:drug effects; D006151:Guanosine / Q000008:administration & dosage / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D009043:Motor Activity / Q000187:drug effects; D009498:Neurotoxins; D011930:Reaction Time / Q000187:drug effects; D012640:Seizures / Q000139:chemically induced / Q000401:mortality / Q000517:prevention & control*
chemical_list: D014151:Anti-Anxiety Agents; D000927:Anticonvulsants; D004546:Elapid Venoms; D009498:Neurotoxins; D006151:Guanosine; C020273:dendrotoxin