Persephonella hydrogeniphila sp. nov., a novel thermophilic, hydrogen-oxidizing bacterium from a deep-sea hydrothermal vent chimney.
A novel thermophilic, hydrogen-oxidizing bacterium, designated 29W(T), was isolated from a deep-sea hydrothermal vent chimney sample collected from the Suiyo Seamount in the Izu-Bonin Arc, Japan, at a depth of 1385 m. The cells were cocci (0.9-1.0 microm in diameter) and straight rods (2.3-2.7 microm long) under static and agitated culture conditions, respectively. The new isolate was an obligate chemolithoautotroph growing by respiratory nitrate reduction with H2, forming N2 as a final product. A very low concentration of O2 (optimum 0.6-0.8%, v/v) was also used as an alternative electron acceptor while reduced sulfur compounds did not serve as electron donors. Anoxic hydrogen-oxidizing growth with nitrate was observed between 50 and 72.5 degrees C (optimum 70 degrees C; 40 min doubling time), pH 5.5 and 7.6 (optimum pH 7.2), and in the presence of 1.5 and 5.0% NaCl (optimum 2.5%). The G + C content of the genomic DNA was 37.3 mol%. Phylogenetic analysis based on 16S rDNA sequences indicated that the isolate was a member of the recently described genus Persephonella in a potential new family within the order Aquificales. On the basis of the physiological and molecular properties of the new isolate, the name Persephonella hydrogeniphila sp. nov. is proposed. The type strain is strain 29W(T) (= JCM 11663(T) = DSM 15103(T)).
Nakagawa
Satoshi
S
Laboratory of Marine Microbiology, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
Takai
Ken
K
Subground Animalcule Retrieval (SUGAR) Project, Frontier Research System for Extremophiles, Japan Marine Science and Technology Center, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan.
Horikoshi
Koki
K
Subground Animalcule Retrieval (SUGAR) Project, Frontier Research System for Extremophiles, Japan Marine Science and Technology Center, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan.
Sako
Yoshihiko
Y
Laboratory of Marine Microbiology, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
D015373:Bacterial Typing Techniques; D001482:Base Composition; D003470:Culture Media; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D006090:Gram-Negative Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D006358:Hot Temperature; D006859:Hydrogen / Q000378:metabolism*; D007564:Japan; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA
D003470:Culture Media; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D006859:Hydrogen