title: Encapsulation of nodal cuttings and shoot tips for storage and exchange of cassava germplasm.
abstract: We report the encapsulation of in vitro-derived nodal cuttings or shoot tips of cassava in 3% calcium alginate for storage and germplasm exchange purposes. Shoot regrowth was not significantly affected by the concentration of sucrose in the alginate matrix while root formation was. In contrast, increasing the sucrose concentration in the calcium chloride polymerisation medium significantly reduced regrowth from encapsulated nodal cuttings of accession TME 60444. Supplementing the alginate matrix with increased concentrations of 6-benzylaminopurine and alpha-naphthaleneacetic acid enhanced complete plant regrowth within 2 weeks. Furthermore, plant regrowth by encapsulated nodal cuttings and shoot tips was significantly affected by the duration of the storage period as shoot recovery decreased from almost 100% to 73.3% for encapsulated nodal cuttings and 94.4% to 60% for shoot tips after 28 days of storage. The high frequency of plant regrowth from alginate-coated micropropagules coupled with high viability percentage after 28 days of storage is highly encouraging for the exchange of cassava genetic resources. Such encapsulated micropropagules could be used as an alternative to synthetic seeds derived from somatic embryos.
authors.affiliation: School of Biosciences, University of Birmingham, Edgbaston, B15 2TT, Birmingham, UK.
authors.forename: K E
authors.initials: KE
authors.lastname: Danso
authors.forename: B V
authors.initials: BV
authors.lastname: Ford-Lloyd
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000464:Alginates / Q000494:pharmacology; D001593:Benzyl Compounds; D004305:Dose-Response Relationship, Drug; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D007701:Kinetin; D002365:Manihot / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development*; D018519:Meristem / Q000187:drug effects / Q000254:growth & development; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development*; D018547:Plant Stems / Q000187:drug effects / Q000254:growth & development*; D011687:Purines; D013395:Sucrose / Q000494:pharmacology; D013997:Time Factors
chemical_list: D000464:Alginates; D001593:Benzyl Compounds; D006603:Hexuronic Acids; D009280:Naphthaleneacetic Acids; D010937:Plant Growth Regulators; D011687:Purines; C034182:1-naphthaleneacetic acid; D013395:Sucrose; D020723:Glucuronic Acid; D000225:Adenine; C480551:benzylaminopurine; D007701:Kinetin