title: Phylogenetic relationship of psychoactive fungi based on the rRNA gene for a large subunit and their identification using the TaqMan assay.
abstract: "Magic mushrooms" (MMs) are psychoactive fungi containing the and Psychotropics Control Law in Japan. Because there are many kinds of MMs and they are often sold even as dry powders in local markets, it is very difficult to identify the original species of the MMs by morphological observation. Therefore, we investigated the rRNA gene for a large subunit (LSU) of several MMs to classify them by a genetic approach. In this paper, we described the phylogeny of species of MMs based on the partial sequence (about 970 bp) of the LSU and the rapid identification of MMs using the TaqMan PCR assay.
authors.affiliation: National Institute of Health Sciences, Tokyo, Japan.
authors.forename: Takuro
authors.initials: T
authors.lastname: Maruyama
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Yokoyama
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Makino
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Goda
mesh_terms: D000363:Agaricales / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D005800:Genes, Fungal / Q000235:genetics*; D020459:Genes, rRNA / Q000235:genetics*; D006213:Hallucinogens / Q000032:analysis* / Q000145:classification; D008969:Molecular Sequence Data; D010802:Phylogeny; D012331:RNA, Fungal / Q000235:genetics*; D013045:Species Specificity
chemical_list: D006213:Hallucinogens; D012331:RNA, Fungal