Neocortical long-term potentiation and experience-dependent synaptic plasticity require alpha-calcium/calmodulin-dependent protein kinase II autophosphorylation.
Experience-dependent plasticity can be induced in the barrel cortex by removing all but one whisker, leading to potentiation of the neuronal response to the spared whisker. To determine whether this form of potentiation depends on synaptic plasticity, we studied long-term potentiation (LTP) and sensory-evoked potentials in the barrel cortex of alpha-calcium/calmodulin-dependent protein kinase II (alphaCaMKII)T286A mutant mice. We studied three different forms of LTP induction: theta-burst stimulation, spike pairing, and postsynaptic depolarization paired with low-frequency presynaptic stimulation. None of these protocols produced LTP in alphaCaMKIIT286A mutant mice, although all three were successful in wild-type mice. To study synaptic plasticity in vivo, we measured sensory-evoked potentials in the barrel cortex and found that single-whisker experience selectively potentiated synaptic responses evoked by sensory stimulation of the spared whisker in wild types but not in alphaCaMKIIT286A mice. These results demonstrate that alphaCaMKII autophosphorylation is required for synaptic plasticity in the neocortex, whether induced by a variety of LTP induction paradigms or by manipulation of sensory experience, thereby strengthening the case that the two forms of plasticity are related.
Hardingham
Neil
N
School of Biosciences, Cardiff University, Cardiff CF10 3US, United Kingdom.
Glazewski
Stanislaw
S
Pakhotin
Pavel
P
Mizuno
Keiko
K
Chapman
Paul F
PF
Giese
K Peter
KP
Fox
Kevin
K
D000818:Animals; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics / Q000378:metabolism*; D004558:Electric Stimulation / Q000379:methods; D005071:Evoked Potentials / Q000502:physiology; D005260:Female; D066298:In Vitro Techniques; D017774:Long-Term Potentiation / Q000502:physiology*; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D009154:Mutation; D019579:Neocortex / Q000201:enzymology / Q000502:physiology*; D009473:Neuronal Plasticity / Q000502:physiology*; D018408:Patch-Clamp Techniques; D010766:Phosphorylation; D010812:Physical Stimulation; D012683:Sensory Deprivation / Q000502:physiology; D013003:Somatosensory Cortex / Q000201:enzymology / Q000502:physiology; D013569:Synapses / Q000502:physiology*; D013826:Theta Rhythm; D014738:Vibrissae / Q000502:physiology
D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases