title: Effects of dietary protein on the activity and mRNA level of trypsin in the midgut gland of the white shrimp Penaeus vannamei.
abstract: Protein food modulates the activity of proteases of the midgut gland of Penaeus vannamei. Shrimp fed with food containing 15, 30 and 50% protein exhibited differences in trypsin and chymotrypsin activity and trypsin mRNA levels. Shrimp fed with 30% protein showed higher trypsin and chymotrypsin activities than those fed 15 or 50% protein. An additional paralogue trypsin was observed with electrophoretic analysis in shrimp fed 30% protein. Shrimp fed 30% protein showed the highest trypsin to mRNA concentration, suggesting that trypsin genes expression is regulated transcriptionally.
authors.affiliation: Laboratorio de Bioquímica, Centro de Investigaciones Biológicas del Noroeste, A.P. 128, La Paz, Baja California Sur 23000, Mexico.
authors.forename: Adriana
authors.initials: A
authors.lastname: Muhlia-Almazán
authors.forename: Fernando L
authors.initials: FL
authors.lastname: García-Carreño
authors.forename: J Arturo
authors.initials: JA
authors.lastname: Sánchez-Paz
authors.forename: Gloria
authors.initials: G
authors.lastname: Yepiz-Plascencia
authors.forename: Alma B
authors.initials: AB
authors.lastname: Peregrino-Uriarte
mesh_terms: D000818:Animals; D001835:Body Weight; D002918:Chymotrypsin / Q000032:analysis / Q000096:biosynthesis; D004044:Dietary Proteins / Q000494:pharmacology*; D004064:Digestive System / Q000201:enzymology*; D005786:Gene Expression Regulation; D009929:Organ Size; D033561:Penaeidae / Q000033:anatomy & histology / Q000201:enzymology* / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism; D014357:Trypsin / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*
chemical_list: D004044:Dietary Proteins; D012333:RNA, Messenger; D012269:Ribosomal Proteins; C060232:ribosomal protein L21; D002918:Chymotrypsin; D014357:Trypsin