title: On the origins of cyanuric acid hydrolase: purification, substrates, and prevalence of AtzD from Pseudomonas sp. strain ADP.
abstract: Cyanuric acid hydrolase (AtzD) from Pseudomonas sp. strain ADP was purified to homogeneity. Of 22 cyclic amides and triazine compounds tested, only cyanuric acid and N-methylisocyanuric acid were substrates. Other cyclic amidases were found not to hydrolyze cyanuric acid. Ten bacteria that use cyanuric acid as a sole nitrogen source for growth were found to contain either atzD or trzD, but not both genes.
authors.affiliation: Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, St. Paul, Minnesota 55108, USA.
authors.forename: Isaac
authors.initials: I
authors.lastname: Fruchey
authors.forename: Nir
authors.initials: N
authors.lastname: Shapir
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Sadowsky
authors.forename: Lawrence P
authors.initials: LP
authors.lastname: Wackett
mesh_terms: D000581:Amidohydrolases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D019143:Evolution, Molecular; D006090:Gram-Negative Bacteria / Q000201:enzymology / Q000235:genetics; D006540:Herbicides / Q000378:metabolism; D011549:Pseudomonas / Q000201:enzymology* / Q000235:genetics*; D013379:Substrate Specificity; D014227:Triazines / Q000737:chemistry / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D006540:Herbicides; D014227:Triazines; D000581:Amidohydrolases; C067141:cyanuric acid amidohydrolase; C004632:cyanuric acid