Quantitative polymerase chain reaction-based assay for estimating DNA yield extracted from domestic cat specimens.
A quantitative polymerase chain reaction (PCR) assay has been developed for the quantification of genomic DNA extracted from domestic cat samples. The assay, which targets highly repetitive genomic short interspersed nuclear elements (SINE), can be performed rapidly and is highly sensitive, detecting as little as 10 fg of feline genomic DNA. The assay was linear over a 10(6) dilution range. We have recently developed a short tandem repeat (STR) multiplex panel for forensic analysis of feline specimens. The SINE assay is an integral part of the forensic typing system. The sensitivity of the assay will enable forensic examiners to determine the likelihood of success of genotyping sample extracts with the STR panel without sacrificing valuable DNA necessary to perform genotyping of samples.
Menotti-Raymond
Marilyn
M
Laboratory of Genomic Diversity, National Cancer Institute, Frederick, MD 21702-1201, USA. raymond@ncifcrf.gov
David
Victor
V
Wachter
Leslie
L
Yuhki
Naoya
N
O'Brien
Stephen J
SJ
D000818:Animals; D002415:Cats / Q000235:genetics*; D016172:DNA Fingerprinting / Q000662:veterinary*; D005554:Forensic Medicine; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D020086:Short Interspersed Nucleotide Elements