A novel aspartate protease gene, ALP56, is related to morphological features of colorectal adenomas.
OBJECTIVE
The recently identified aspartate protease gene ALP56 is up-regulated in human malignant tumors, including colorectal cancers, but the relationship remain unclear between ALP56 gene expression and clinicopathological findings, as well as when genetic alterations in ALP56 occur during the colorectal adenoma-carcinoma sequence. We therefore investigated expression of ALP56 mRNA in various human colorectal tissues.


METHODS
We examined 18 colorectal adenomas 22 cancers, and 24 adjacent normal mucosal samples from patients undergoing conventional resection or endoscopic mucosal resection. Expression of ALP56 mRNA was determined by quantitative reverse-transcription polymerase chain reaction.


RESULTS
Up-regulation of ALP56 gene transcription was observed in both adenomas and cancers compared to normal mucosa. ALP56 expression in exophytic adenomas was significantly greater than in flat adenomas.


CONCLUSIONS
ALP56 may contribute to colorectal adenoma formation and to an exophytic growth pattern in these adenomas.
Tsuji
Naoki
N
Department of Clinical Laboratory Medicine and Division of Laboratory Diagnosis, School of Medicine, Sapporo Medical University, South-1 West-16, Chuo-ku, 060-8543, Sapporo, Japan.
Kondoh
Kei
K
Furuya
Momoko
M
Kobayashi
Daisuke
D
Yagihashi
Atsuhito
A
Inoue
Yoshiyuki
Y
Meguro
Takashi
T
Horita
Shoichi
S
Takahashi
Hiroshi
H
Watanabe
Naoki
N
D000236:Adenoma / Q000235:genetics*; D000368:Aged; D000369:Aged, 80 and over; D016282:Aspartic Acid Endopeptidases / Q000737:chemistry / Q000235:genetics*; D016022:Case-Control Studies; D002402:Cathepsin D / Q000737:chemistry / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D006801:Humans; D007413:Intestinal Mucosa / Q000473:pathology; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020539:Sequence Analysis, Protein; D015854:Up-Regulation / Q000235:genetics
D012333:RNA, Messenger; C411442:ALP56 enzyme; D016282:Aspartic Acid Endopeptidases; D002402:Cathepsin D