Multiplex PCR assay for identification of human diarrheagenic Escherichia coli.
A multiplex PCR assay for the identification of human diarrheagenic Escherichia coli was developed. The targets selected for each category were eae for enteropathogenic E. coli, stx for Shiga toxin-producing E. coli, elt and est for enterotoxigenic E. coli, ipaH for enteroinvasive E. coli, and aggR for enteroaggregative E. coli. This assay allowed the categorization of a diarrheagenic E. coli strain in a single reaction tube.
Toma
Claudia
C
Department of Bacteriology, Faculty of Medicine, University of the Ryukyus, Nishihara, Okinawa 903-0215, Japan. k950417@med.u-ryukyu.ac.jp
Lu
Yan
Y
Higa
Naomi
N
Nakasone
Noboru
N
Chinen
Isabel
I
Baschkier
Ariela
A
Rivas
Marta
M
Iwanaga
Masaaki
M
D003967:Diarrhea / Q000382:microbiology*; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D037521:Virulence Factors / Q000235:genetics
D029968:Escherichia coli Proteins; D037521:Virulence Factors