Incorporation of bovine bone marrow stromal cells into porcine foetal tissues after xenotransplantation.
Bovine bone marrow stromal cells (BMSCs) were injected into the liver of foetal pigs at about 40 days of gestation to test whether these cells could populate developing tissue, and if so, which ones. Approximately 40 days after injection, the foetuses were harvested and tissue sections from many areas of the body were analysed for the presence of bovine cells using two different methods. First, using PCR, bovine repetitive DNA was found to be present in DNA extracted from foetal pig tissues. Secondly, using oligonucleotide primed in situ synthesis (PRINS), the in situ presence of bovine cells was found within porcine tissue sections. PRINS-labelled cells were found within cartilage, perichondrium, connective tissue and smooth muscle. These data suggest that bovine BMSCs integrate throughout the foetal pig.
Troyer
D L
DL
Department of Anatomy and Physiology, Kansas State University, Manhattan, KA 66506-5802, USA. troyer@vet.ksu.edu
Weiss
M L
ML
Mitchell
K E
KE
Martin
P L
PL
Davis
D L
DL
D000818:Animals; D016026:Bone Marrow Transplantation; D002417:Cattle / Q000235:genetics*; D004247:DNA / Q000032:analysis; D017931:DNA Primers; D005314:Embryonic and Fetal Development; D005333:Fetus; D008297:Male; D016133:Polymerase Chain Reaction / Q000662:veterinary; D017154:Stromal Cells / Q000637:transplantation; D013552:Swine / Q000196:embryology / Q000235:genetics*; D014183:Transplantation, Heterologous / Q000379:methods / Q000662:veterinary*
D017931:DNA Primers; D004247:DNA