Role of the non-mevalonate pathway in indole alkaloid production by Catharanthus roseus hairy roots.
The 1-deoxy-D-xylulose-5-phosphate (DXP) pathway (non-mevalonate pathway) leading to terpenoids via isopentenyl diphosphate (IPP) has been shown to occur in most bacteria and in all higher plants. Treatment with the antibiotic fosmidomycin, a specific inhibitor of DXP reductoisomerase, considerably inhibited the accumulation of the alkaloids ajmalicine, tabersonine, and lochnericine by Catharanthus roseus hairy root cultures in the exponential growth phase. However, fosmidomycin did not significantly affect alkaloid levels in stationary phase hairy root cultures. Feeding with 1-deoxy-D-xylulose, 10-hydroxygeraniol, or loganin resulted in significant increases in alkaloid production by exponential phase hairy root cultures. These results suggest that the DXP pathway is a major provider of carbon for the monoterpenoid pathway leading to the formation of indole alkaloids in C. roseus hairy roots in the exponential phase.
Hong
Seung-Beom
SB
Department of Biochemistry and Cell Biology, Rice University, MS-140, 6100 Main St., Houston, Texas 77005, USA.
Hughes
Erik H
EH
Shanks
Jacqueline V
JV
San
Ka-Yiu
KY
Gibson
Susan I
SI
D030008:Catharanthus / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D005578:Fosfomycin / Q000031:analogs & derivatives* / Q000494:pharmacology; D045782:Hemiterpenes / Q000378:metabolism*; D026121:Indole Alkaloids / Q000378:metabolism*; D008660:Metabolism / Q000502:physiology*; D008798:Mevalonic Acid / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism*; D010428:Pentosephosphates / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism*; D013729:Terpenes / Q000378:metabolism*
C109460:1-deoxylulose 5-phosphate; D045782:Hemiterpenes; D026121:Indole Alkaloids; D009097:Multienzyme Complexes; D009943:Organophosphorus Compounds; D010428:Pentosephosphates; D013729:Terpenes; D005578:Fosfomycin; C004809:isopentenyl pyrophosphate; C024640:fosmidomycin; D008798:Mevalonic Acid