Biological characterization of experimental carbon samples.
The use of carbon is widespread in fields as wide as aeronautics, cars, electricity or electronics. The biomedical applications of carbon are also numerous. The purpose of our work is to test four experimental carbon fibers (A, B, C and D; B being the negative control) to determine the best clinical application. Four tests of cytocompatibility are carried out (cell viability, inflammatory test, cell proliferation and cell morphology). Two different cell lines are used: the L132 cell line (epithelial embryonic pulmonary human cell) and the HaCaT line (human normal spontaneously immortalized skin keratinocytes). The results of the biological tests are compared with those of a carbon fiber sample already marketed as a bandage in the treatment of infected wounds: Actisorb "Plus (J2). The various tests show us that only two experimental samples are slightly cytotoxic (A, D). On the other hand, no sample supports cell adherence. A, B, C and D do not have an inflammatory effect. J2 appears at the same time cytotoxic and inflammatory. Consequently, being given the physical presentation and the biological properties of experimental samples (A, C and D), we intend them for an application in the field of wound healing, as a bandage. Also further experimentation is needed.
Delfosse
C
C
Faculté d'Odontologie, Groupe de Recherche sur les Biomatériaux, Faculté de médecine, place de Verdun, Lille, France. cdelfosse@univ-lille2.fr
Monchau
F
F
Lefevre
A
A
Maquin
D
D
Lafforgue
P
P
Hildebrand
H F
HF
D001672:Biocompatible Materials / Q000737:chemistry* / Q000633:toxicity; D002244:Carbon / Q000737:chemistry* / Q000633:toxicity; D000077482:Carbon Fiber; D002448:Cell Adhesion; D002455:Cell Division; D002460:Cell Line; D048429:Cell Size; D002470:Cell Survival; D004847:Epithelial Cells / Q000166:cytology; D015726:Giant Cells / Q000473:pathology; D006801:Humans; D015603:Keratinocytes / Q000166:cytology; D008168:Lung / Q000166:cytology / Q000196:embryology; D012867:Skin / Q000166:cytology; D013499:Surface Properties; D014617:Vacuoles / Q000648:ultrastructure
D001672:Biocompatible Materials; D000077482:Carbon Fiber; D002244:Carbon