Synthesis of complex oligosaccharides by using a mutated (1,3)-beta-D-glucan endohydrolase from barley.
Complex oligosaccharides with newly formed (1,3)-beta-glycosidic linkages were obtained in good to excellent yields when substituted or unsubstituted alpha-laminaribiosyl fluorides, acting as donors, were condensed onto mono- and disaccharide beta-D-hexopyranoside acceptors by using a (1,3)-beta-D-glycosynthase. These linear and branched (1,3)-beta-linked oligosaccharides could prove to be important in a range of medical, pharmaceutical, and agricultural applications. Furthermore, the observation that the (1,3)-beta-D-glucan glycosynthase accommodates (1,3)-, (1,4),- and (1,6)-beta-oligosaccharides in its acceptor subsites suggests novel, yet unexpected physiological roles for the wild type (1,3)-beta-D-glucan endohydrolase from higher plants.
Fairweather
Jon K
JK
Centre de Recherches sur les Macromolécules Végétales (CERMAV-CNRS), affiliated with Université J. Fourier, B.P. 53, Grenoble Cedex 9, 38041 France.
Hrmova
Maria
M
Rutten
Simon J
SJ
Fincher
Geoffrey B
GB
Driguez
Hugues
H
D019943:Amino Acid Substitution; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002384:Catalysis; D004187:Disaccharides / Q000737:chemistry; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism*; D006027:Glycosides / Q000096:biosynthesis / Q000737:chemistry; D001467:Hordeum / Q000201:enzymology*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000737:chemistry
D004187:Disaccharides; D006027:Glycosides; D009844:Oligosaccharides; D006026:Glycoside Hydrolases