Micro-assembly of functionalized particulate monolayer on C18-derivatized SiO2 surfaces.
This work describes a simple approach to immobilize functionalized colloidal microstructures onto a C(18)-coated SiO(2) substrate via specific or non-specific bio-mediated interactions. Biotinylated bovine serum albumin pre-adsorbed onto a C(18) surface was used to mediate the surface assembly of streptavidin-coated microbeads (2.8 microm), while a bare C(18) surface was used to immobilize anti-Listeria antibody-coated microbeads (2.8 microm) through hydrophobic interactions. For a C(18) surface pre-adsorbed with bovine serum albumin, hydrophobic polystyrene microbeads (0.8 microm) and positively charged dimethylamino microbeads (0.8 microm) were allowed to self-assemble onto the surface. A monolayer with high surface coverage was observed for both polystyrene and dimethylamino microbeads. The adsorption characteristics of Escherichia coli and Listeria monocytogenes on these microbead-based surfaces were studied using fluorescence microscopy. Both streptavidin microbeads pre-adsorbed with biotinylated anti-Listeria antibody and anti-Listeria antibody-coated microbeads showed specific capture of L. monocytogenes, while polystyrene and dimethylamino microbeads captured both E. coli and L. monocytogenes non-specifically. The preparation of microbead-based surfaces for the construction of microfluidic devices for separation, detection, or analysis of specific biological species is discussed.
Huang
Tom T
TT
School of Chemical Engineering and Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, Indiana 47907, USA.
Geng
Tao
T
Akin
Demir
D
Chang
Woo-Jin
WJ
Sturgis
Jennifer
J
Bashir
Rashid
R
Bhunia
Arun K
AK
Robinson
J Paul
JP
Ladisch
Michael R
MR
D000327:Adsorption; D001422:Bacterial Adhesion / Q000502:physiology; D018441:Biofilms / Q000254:growth & development*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis; D004926:Escherichia coli / Q000166:cytology / Q000502:physiology*; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods; D008089:Listeria monocytogenes / Q000166:cytology / Q000502:physiology*; D008567:Membranes, Artificial; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D008863:Microspheres; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012821:Silanes / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry
D020099:Coated Materials, Biocompatible; D008567:Membranes, Artificial; D012821:Silanes; C061189:octadecyltrichlorosilane; D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide; D019809:Streptavidin