title: Identification of the Anopheles gambiae ATP-binding cassette transporter superfamily genes.
abstract: The Anopheles gambiae genome sequence has been analyzed to find ATP-binding cassette protein genes based on deduced protein similarity to known family members. A nonredundant collection of 44 putative genes was identified including five genes not detected by the original Anopheles genome project machine annotation. These genes encode at least one member of all the human and Drosophila melanogaster ATP-binding protein subgroups. Like D. melanogaster, A. gambiae has subgroup ABCH genes encoding proteins different from the ABC proteins found in other complex organisms. The largest Anopheles subgroup is the ABCC genes which includes one member that can potentially encode ten different isoforms of the protein by differential splicing. As with Drosophila, the second largest Anopheles group is the ABCG subgroup with 12 genes compared to 15 genes in D. melanogaster, but only 5 genes in the human genome. In contrast, fewer ABCA and ABCB genes were identified in the mosquito genome than in the human or Drosophila genomes. Gene duplication is very evident in the Anopheles ABC genes with two groups of four genes, one group with three genes and three groups with two head to tail duplicated genes. These characteristics argue that the A. gambiae is actively using gene duplication as a mechanism to drive genetic variation in this important gene group.
authors.affiliation: Unité de Biochimie et Biologie Moleculaire des Insectes, Institut Pasteur, 75724 Paris Cedex 15, France. croth@pasteur.fr
authors.forename: Charles W
authors.initials: CW
authors.lastname: Roth
authors.forename: Inge
authors.initials: I
authors.lastname: Holm
authors.forename: Marine
authors.initials: M
authors.lastname: Graille
authors.forename: Pierre
authors.initials: P
authors.lastname: Dehoux
authors.forename: Andrey
authors.initials: A
authors.lastname: Rzhetsky
authors.forename: Patrick
authors.initials: P
authors.lastname: Wincker
authors.forename: Jean
authors.initials: J
authors.lastname: Weissenbach
authors.forename: Paul T
authors.initials: PT
authors.lastname: Brey
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000818:Animals; D000852:Anopheles / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics; D005136:Eye Proteins / Q000235:genetics; D005819:Genetic Markers; D006801:Humans; D005810:Multigene Family; D010802:Phylogeny
chemical_list: D018528:ATP-Binding Cassette Transporters; D029721:Drosophila Proteins; D005136:Eye Proteins; D005819:Genetic Markers; C059254:w protein, Drosophila