Expression of Netrin-1 and its two receptors DCC and UNC5H2 in the developing mouse lung.
The ligand Netrin-1 and its receptors DCC and UNC5H2 are critical for the regulation of neuronal migration in nervous system development. Here we demonstrate expression of these molecules in lung development. The mRNA expression profiles of Netrin-1, DCC and UNC5H2 are developmentally regulated during embryonic mouse lung formation. Netrin-1 shows a bimodal expression pattern with elevated mRNA levels early followed by a second peak in late gestation. Peak expression of DCC occurs early in development whereas expression of UNC5H2 peaks late in development. We also demonstrate localization of Netrin-1, DCC and UNC5H2 during the stages of lung development. We present evidence that these proteins are modulated spatially in the mesenchyme and epithelium during lung organogenesis.
Dalvin
Sussie
S
Department of Pediatrics, Massachusetts General Hospital for Children, Harvard Medical School, Boston, MA 02114, USA.
Anselmo
Mark A
MA
Prodhan
Parthak
P
Komatsuzaki
Katsumi
K
Schnitzer
Jay J
JJ
Kinane
T Bernard
TB
D000818:Animals; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000235:genetics*; D000075389:DCC Receptor; D005260:Female; D007150:Immunohistochemistry; D008168:Lung / Q000196:embryology* / Q000378:metabolism; D051379:Mice; D009414:Nerve Growth Factors / Q000096:biosynthesis / Q000235:genetics*; D000075386:Netrin Receptors; D000075388:Netrin-1; D037701:Pulmonary Surfactant-Associated Protein B / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis / Q000235:genetics*
D015815:Cell Adhesion Molecules; D000075389:DCC Receptor; D009414:Nerve Growth Factors; D000075386:Netrin Receptors; D000075388:Netrin-1; D037701:Pulmonary Surfactant-Associated Protein B; D011956:Receptors, Cell Surface; D025521:Tumor Suppressor Proteins; C501910:Dcc protein, mouse; C000617353:Ntn1 protein, mouse; C000617307:Unc5b protein, mouse