Evaluation of a new pretreatment reagent for use with the EMIT 2000 cyclosporine assay.
OBJECTIVE
Evaluate the performance of the new pretreatment (NPT) reagent for use with the EMIT cyclosporine A (CsA) assay.


METHODS
Samples from transplant patients receiving CsA were tested using a COBAS MIRA S.


RESULTS
A downward shift in target values for commercial controls was observed using the NPT reagent. There was excellent correlation for patient results when comparing the NPT reagent with methanol pretreatment.


CONCLUSIONS
The NPT reagent is an acceptable substitute for methanol in the EMIT(R) extraction procedure for CsA.
Butch
Anthony W
AW
Department of Pathology & Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095-1713, USA. abutch@mednet.ucla.edu
Fukuchi
Alan M
AM
D016572:Cyclosporine / Q000097:blood*; D017248:Enzyme Multiplied Immunoassay Technique / Q000295:instrumentation / Q000592:standards*; D006801:Humans; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D015203:Reproducibility of Results
D011933:Reagent Kits, Diagnostic; D016572:Cyclosporine