title: [Expression and function of zinc ribbon gene ZNRD1 in drug-resistant gastric cancer cells].
abstract: OBJECTIVE
  To study the expression and function of zinc ribbon gene ZNRD1 in drug-resistant cells of gastric cancer.
  
  
  METHODS
  Two tumor cell lines were used in this study: gastric cancer SGC7901 and its drug-resistant counterpart SGC7901/VCR stepwise-selected by vincristine. The expression of ZNRD1 in SGC7901 and SGC7901/VCR was detected by northern blot and semiquantitative RT-PCR. ZNRD1 antisense nucleic acid was transfected into SGC7901/VCR cells by lipofectamine. The expression of protein in SGC7901/VCR cells and the transfectants was detected by immunochemical method. Fluorescence activated cell scan (FACS) was applied to observe the cell cycle alteration. Growth curve and drug sensitization of cells for vincristine (VCR) and adriamycin (ADM) were analyzed by MTT assay.
  
  
  RESULTS
  The expression of ZNRD1 was higher in SGC7901/VCR than in SGC7901 cells. Immunochemical results showed that the expression level of ZNRD1 protein was lower in anti ZNRD1-SGC7901/VCR cells than in non-transfectants. The anti ZNRD1-SGC7901/VCR cells were gradually accumulated in G(1) phase, with a concomitant decrease of cell population in S phase. MTT assay showed that transfectant cell proliferation was lagged and more sensitive to VCR and ADM than non-transfectants.
  
  
  CONCLUSIONS
  ZNRD1 gene displays high expression in VCR resistant gastric cancer cells. Expression of ZNRD1 protein is effectively blocked in anti ZNRD1-SGC7901/VCR cells by gene transfection. ZNRD1 antisense nucleic acid could reverse, to some degree, the MDR of human drug-resistant gastric cancer cell SGC7901/VCR.
authors.affiliation: Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi'an 710033, China.
authors.forename: Yu-mei
authors.initials: YM
authors.lastname: Zhang
authors.forename: Yan-qiu
authors.initials: YQ
authors.lastname: Zhao
authors.forename: Quan-jian
authors.initials: QJ
authors.lastname: Yan
authors.forename: Yang-lin
authors.initials: YL
authors.lastname: Pan
authors.forename: Hui
authors.initials: H
authors.lastname: Yi
authors.forename: Dai-ming
authors.initials: DM
authors.lastname: Fan
mesh_terms: D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D004317:Doxorubicin / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm; D006801:Humans; D013274:Stomach Neoplasms / Q000737:chemistry / Q000188:drug therapy / Q000473:pathology*; D014750:Vincristine / Q000494:pharmacology
chemical_list: D004268:DNA-Binding Proteins; C406241:POLR1H protein, human; D014750:Vincristine; D004317:Doxorubicin