title: The possible macrophage role in seminal fluid.
abstract: The present report was designed to study the macrophage role and their association with percentage of spermatozoa showing DNA native, morphologically normal sperm and occurrence of antibody-coated spermatozoa. Human sperm samples from 31 patients were used in this study. All samples were evaluated for standard semen parameters according to World Health Organization criteria. The sperm heads with abnormal versus normal chromatin structure were specified as orange-red versus green by Acridine Orange method. Macrophage concentration was assessed with Neutral Red in Neubauer hemocytometer. The technique for the determination of sperm bound antisperm antibodies was sperm mixed antiglobulin reaction. In our study the presence of antisperm antibodies in associated to macrophages concentration (p < 0.05). The log of phagocytic cells were highly correlated with an increase of spermatozoa showing DNA denaturation (p < 0.05). The log of phagocytic cells were highly correlated with an increase of spermatozoa showing DNA denaturation (p < 0.05). There isn't significative association between the log macrophage concentration and morphologically normal sperm (p: 0.414). Thus, the present study further emphasizes the importance of immunosurveillance of the macrophages, and the contribution at the semen quality for the success of the fertilization.
authors.affiliation: Dept. of Clinical Biochemistry, University of Biochemistry and Pharmaceutical Sciences, Rosario, Reproduction Service, Eva Peron School Hospital, National University of Rosario, Argentina.
authors.forename: E A
authors.initials: EA
authors.lastname: Solis
authors.forename: B R
authors.initials: BR
authors.lastname: Bouvet
authors.forename: A S
authors.initials: AS
authors.lastname: Brufman
authors.forename: R
authors.initials: R
authors.lastname: Feldman
authors.forename: V N
authors.initials: VN
authors.lastname: Gatti
mesh_terms: D000165:Acridine Orange / Q000032:analysis; D000328:Adult; D001323:Autoantibodies / Q000032:analysis; D002843:Chromatin / Q000737:chemistry; D004247:DNA / Q000032:analysis; D005456:Fluorescent Dyes / Q000032:analysis; D006801:Humans; D008264:Macrophages / Q000502:physiology*; D008297:Male; D009499:Neutral Red; D009691:Nucleic Acid Denaturation; D010587:Phagocytosis; D012661:Semen / Q000166:cytology* / Q000276:immunology; D013077:Sperm Head / Q000737:chemistry / Q000648:ultrastructure; D013094:Spermatozoa / Q000276:immunology / Q000648:ultrastructure; D013194:Staining and Labeling
chemical_list: D001323:Autoantibodies; D002843:Chromatin; D005456:Fluorescent Dyes; D009499:Neutral Red; D004247:DNA; D000165:Acridine Orange