A chimeric mouse model established by allogenic in utero transplantation.
In utero stem cells transplantation is a promising approach to the prenatal treatment of diseases. In order to investigate the fate of the stem cells after in utero transplantation, we have established a chimeric mouse model with the method of in utero transplantation. Mononuclear cells (including stem cells/progenitor cells) derived from male mouse bone marrow were injected into fetal mouse peritoneal cavity during the pre-immune period. The donor cells in the circulatory blood of female recipients were identified by fluorescence in situ hybridization (FISH), and the Y-chromosome specific DNA was detected by PCR as well as real-time quantitative PCR after the recipient mice were born. The results showed that a total of 4 female recipient mice were chimeric in their peripheral blood. Significantly, the donor cells in three chimeric mice persisted up to six months.
Wang
Mo-Lin
ML
Shanghai Institute of Medical Genetics, Shanghai Children's Hospital, Shanghai Communication University, Shanghai 200040, China.
Yan
Jing-Bin
JB
Xiao
Yan-Ping
YP
Ren
Zhao-Rui
ZR
Huang
Shu-Zhen
SZ
Zeng
Yi-Tao
YT
D000818:Animals; D000831:Animals, Newborn; D005260:Female; D005333:Fetus / Q000601:surgery*; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D051379:Mice; D023421:Models, Animal; D011247:Pregnancy; D011256:Pregnancy Outcome; D018183:Transplantation Chimera / Q000097:blood* / Q000235:genetics; D014184:Transplantation, Homologous; D014886:Weaning