Receptor-mediated gene delivery.
Receptor-mediated gene delivery capitalises on the presence of specific cell surface molecules for DNA uptake into cells and represents a particularly appealing approach for targeting vector DNA to specific cell types in vivo and in vitro. Various ligand/DNA and antibody/DNA transfer complexes were generated that, following binding to cells, are internalised and reach the endosomal compartment. Vector complexes contain endosomolytic components that ensure vector release from the endosome and translocation of vector DNA into the nucleus where transcription occurs. Thus, receptor-mediated gene delivery encompasses several critical steps that must be considered when designing and applying such vector systems.
Gust
Tatjana C
TC
Max Delbrück Center for Molecular Medicine (MDC), Robert-Rössle Str. 10, D-13125 Berlin, Germany.
Zenke
Martin
M
D000818:Animals; D001692:Biological Transport; D002467:Cell Nucleus / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D018014:Gene Transfer Techniques; D005821:Genetic Techniques; D015316:Genetic Therapy; D006801:Humans; D011956:Receptors, Cell Surface / Q000378:metabolism*
C033365:DNA receptor; D011956:Receptors, Cell Surface; D004247:DNA