[DNA duplexes containing 2'-deoxy-2'-iodoacetamidouridine as reagents for affinity modification of proteins].
DNA duplexes containing the iodoacetamido group at position 2' of the ribose moiety were proposed for affinity modification of Cys in DNA-binding proteins. Reactive DNA derivatives were obtained with iodoacetic anhydride and synthetic oligodeoxyribonucleotides containing 2'-amino-2'-deoxyuridine in place of thymine at various positions. The derivatives were tested for reaction with amino acids and peptides and shown to specifically interact with Cys-containing proteins. The possibility of using the modified DNA duplexes to probe the protein SH group close to the DNA sugar-phosphate backbone in DNA-protein complexes was demonstrated with the example of subunit p50 of human transcription factor NF-kappa B.
Borisova
O A
OA
Chemical Department and Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, 119992 Russia.
Romanenkov
A S
AS
Metelev
V G
VG
Kubareva
E A
EA
Zubin
E M
EM
Timchenko
M A
MA
Oretskaia
T S
TS
D003545:Cysteine / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D019281:Dimerization; D005982:Glutathione Transferase / Q000737:chemistry / Q000235:genetics; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry*; D007461:Iodoacetates / Q000737:chemistry*; D016328:NF-kappa B / Q000737:chemistry / Q000235:genetics; D052002:NF-kappa B p50 Subunit; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D012266:Ribose / Q000737:chemistry; D014500:Uracil Nucleotides / Q000737:chemistry*
C477500:2'-deoxy-2'-iodoacetamidouridine; D007202:Indicators and Reagents; D007461:Iodoacetates; D016328:NF-kappa B; D052002:NF-kappa B p50 Subunit; D009692:Nucleic Acid Heteroduplexes; D009841:Oligonucleotides; D011506:Proteins; D014500:Uracil Nucleotides; D012266:Ribose; D004247:DNA; D005982:Glutathione Transferase; D003545:Cysteine