Changes in DNA synthesis and morphometric indexes of the myocardium in albino rats treated with angiotensin II.
Autoradiography with 3H-thymidine showed that angiotensin II injected intraperitoneally to newborn albino rats from the 2nd to 6th day of life stimulates DNA synthesis in the myocardium of the left ventricle and increased the area of cardiomyocytes and number and area of nucleoli. Two weeks after treatment the area of cardiomyocytes and the number of nucleoli increased, while the count of PCNA-positive nuclei remained unchanged. Immunohistochemical assay for vimentin showed that the ratio between the number of cardiomyocytes and connective tissue cells remained unchanged. In 45-day-old rats (38 days after treatment) the intensity of DNA synthesis and morphometric indexes of the myocardium did not differ from the control. Fivefold treatment of adult rats with angiotensin II increased the number and area of nucleoli, but had no effect on the count of connective tissue cells.
Mel'nikova
N P
NP
Central Research Laboratory, Far-Eastern State Medical University, Khabarovsk. nnppmm67@mail.ru
Timoshin
S S
SS
Zhivotova
E Yu
EY
D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D002466:Cell Nucleolus / Q000378:metabolism; D004261:DNA Replication; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D014746:Vimentin / Q000378:metabolism
D014746:Vimentin; D000804:Angiotensin II