title: Subgenotype analysis of Cryptosporidium isolates from humans, cattle, and zoo ruminants in Portugal.
abstract: Cryptosporidium parvum and Cryptosporidium hominis isolates from human immunodeficiency virus-infected patients, cattle, and wild ruminants were characterized by PCR and DNA sequencing analysis of the 60-kDa glycoprotein gene. Seven alleles were identified, three corresponding to C. hominis and four corresponding to C. parvum. One new allele was found (IId), and one (IIb) had only been found in Portugal. Isolates from cattle and wild ruminants clustered in two alleles. In contrast, human isolates clustered in seven alleles, showing extensive allelic diversity.
authors.affiliation: Unidade de Protozoários Oportunistas/VIH e Outras Protozooses, Unidade de Parasitologia e Microbiologia Médicas (UPMM), Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, 1349-008 Lisbon, Portugal. malves@ihmt.unl.pt
authors.forename: Margarida
authors.initials: M
authors.lastname: Alves
authors.forename: Lihua
authors.initials: L
authors.lastname: Xiao
authors.forename: Irshad
authors.initials: I
authors.lastname: Sulaiman
authors.forename: Altaf A
authors.initials: AA
authors.lastname: Lal
authors.forename: Olga
authors.initials: O
authors.lastname: Matos
authors.forename: Francisco
authors.initials: F
authors.lastname: Antunes
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000453:epidemiology / Q000469:parasitology*; D000595:Amino Acid Sequence; D000818:Animals; D000836:Animals, Zoo / Q000469:parasitology*; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000469:parasitology*; D003457:Cryptosporidiosis / Q000453:epidemiology / Q000469:parasitology* / Q000662:veterinary; D003458:Cryptosporidium / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016785:Cryptosporidium parvum / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012418:Ruminants / Q000469:parasitology*; D017422:Sequence Analysis, DNA