title: RNA polymerase III transcripts and the PTB protein are essential for the integrity of the perinucleolar compartment.
abstract: The perinucleolar compartment (PNC) is a nuclear substructure present in transformed cells. The PNC is defined by high concentrations of certain RNA binding proteins and a subset of small RNAs transcribed by RNA polymerase III (pol III), including the signal recognition particle RNA and an Alu RNA as reported here. To determine if the PNC is dependent on pol III transcription, HeLa cells were microinjected with the selective pol III inhibitor, Tagetin. This resulted in disassembly of the PNC, whereas inhibition of pol I by cycloheximide or pol II by alpha-amanitin did not significantly affect the PNC. However, overexpression of one of the PNC-associated RNAs from a pol II promoter followed by injection of Tagetin blocked the Tagetin-induced PNC disassembly, demonstrating that it is the RNA rather than pol III activity that is important for the PNC integrity. To elucidate the role of the PNC-associated protein PTB, its synthesis was inhibited by siRNA. This resulted in a reduction of the number of PNC-containing cells and the PNC size. Together, these findings suggest, as a working model, that PNCs may be involved in the metabolism of specific pol III transcripts in the transformed state and that PTB is one of the key elements mediating this process.
authors.affiliation: Department of Cell and Molecular Biology, Northwestern University Medical School, Chicago, Illinois 60611, USA.
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.forename: Joan C
authors.initials: JC
authors.lastname: Politz
authors.forename: Thoru
authors.initials: T
authors.lastname: Pederson
authors.forename: Sui
authors.initials: S
authors.lastname: Huang
mesh_terms: D020087:Alu Elements / Q000235:genetics; D002467:Cell Nucleus / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D038941:Polypyrimidine Tract-Binding Protein / Q000378:metabolism*; D012313:RNA / Q000378:metabolism; D012320:RNA Polymerase III / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering; D018271:Signal Recognition Particle / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering; D018271:Signal Recognition Particle; D038941:Polypyrimidine Tract-Binding Protein; D012313:RNA; D012320:RNA Polymerase III