title: Growth dynamics of Artemisia annua hairy roots in three culture systems.
abstract: The transient growth of Artemisia annua hairy roots was compared for cultures grown in shake flasks and in bubble column and mist reactors. Instantaneous growth rates were obtained by numerically differentiating the transient biomass measurements. Specific sugar consumption rates showed good agreement with literature values. From the growth rate and sugar consumption rate, the specific yield and maintenance coefficient for sugar were determined for all three culture systems. These values were statistically indistinguishable for roots grown in shake flasks and bubble columns. In contrast, the values for roots grown in bubble columns and mist reactors were statistically different, suggesting that sugar utilization by roots grown in these two systems may be different. By measuring respiration rates in the bubble column reactor we also determined the actual biomass yield and maintenance coefficient for O(2) and CO(2). Together with an elemental analysis of the roots, this allowed us to obtain a reasonable carbon balance.
authors.affiliation: Department of Chemical Engineering, Worcester Polytechnic Institute, 100 Institute Road, Worcester, Massachusetts 01609, USA.
authors.forename: Yoo Jeong
authors.initials: YJ
authors.lastname: Kim
authors.forename: Pamela J
authors.initials: PJ
authors.lastname: Weathers
authors.forename: Barbara E
authors.initials: BE
authors.lastname: Wyslouzil
mesh_terms: D031172:Artemisia annua / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D019149:Bioreactors / Q000145:classification*; D050260:Carbohydrate Metabolism; D002455:Cell Division / Q000502:physiology; D003198:Computer Simulation; D046508:Culture Techniques / Q000295:instrumentation* / Q000379:methods*; D008954:Models, Biological; D009584:Nitrogen / Q000378:metabolism; D010101:Oxygen Consumption / Q000502:physiology; D018517:Plant Roots / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D009584:Nitrogen