Effects of ketotifen 0.025% and lodoxamide 0.1% on eosinophil infiltration into the guinea pig conjunctiva in a model of allergic conjunctivitis.
The effects of ketotifen and lodoxamide on eosinophil infiltration were assessed in a guinea pig model of allergic conjunctivitis. The two active treatments were coded in this masked study in which 30 male guinea pigs, sensitized to chicken egg albumin (ovalbumin), were randomly assigned to one of three groups: Group 1, instillation of 0.9% NaCl into the conjunctival sac of both eyes; Group 2, instillation of 0.025% ketotifen into the left eye and 0.9% NaCl into the right eye; Group 3, instillation of 0.1% lodoxamide into the left eye and 0.9% NaCl into the right eye. Ovalbumin was administered topically to each eye, except in Group 1 where it was only applied to the left eye. (111)In-oxine labeled eosinophils were injected into the jugular vein of each guinea pig; the animals were sacrificed 17 hours after ovalbumin had been applied. The level of radioactivity in the ketotifen- and lodoxamide-treated eyes was approximately 60% of that in the saline-treated eyes. Moreover, the mean level of radioactivity in the ketotifen- and lodoxamide-treated eyes was comparable with the mean level of radioactivity in the saline-treated eye of Group 1, which had not been exposed to allergen. These results indicate that the therapeutic effects of ketotifen and lodoxamide in allergic conjunctivitis may be partly mediated by an inhibitory effect on eosinophils.
Schoch
Christian
C
Novartis Ophthalmics AG, Basel, Switzerland. christian-schoch@pharma.novartis.com
D000287:Administration, Topical; D000818:Animals; D018926:Anti-Allergic Agents / Q000008:administration & dosage*; D002465:Cell Movement / Q000187:drug effects; D003233:Conjunctivitis, Allergic / Q000276:immunology / Q000473:pathology*; D004804:Eosinophils / Q000473:pathology* / Q000502:physiology*; D006168:Guinea Pigs; D007665:Ketotifen / Q000008:administration & dosage*; D008297:Male; D010047:Ovalbumin / Q000276:immunology; D010072:Oxamic Acid / Q000008:administration & dosage* / Q000031:analogs & derivatives*
D018926:Anti-Allergic Agents; D010047:Ovalbumin; D010072:Oxamic Acid; C021702:lodoxamide ethyl; D007665:Ketotifen