Kinetic characterization of carrier-mediated transport systems for D-glucose and taurocholate in the everted sacs of the rat colon.
The present study was aimed at kinetically characterizing the carrier-mediated transport systems for D-glucose and taurocholate in the rat colon, compared with their respective counterparts in the small intestine. The transport of these compounds was evaluated by measuring the initial uptake into everted intestinal tissue sacs. The uptake of both D-glucose and taurocholate was highly saturable, conforming to Michaelis-Menten kinetics without an appreciable nonsaturable transport component. The Michaelis constant (K(m)) was 0.43 and 0.021 mM, respectively, for D-glucose and taurocholate and the maximum transport rate (J(max)) was 0.82 and 0.056 nmol/min/100 mg wet tissue weight (wtw), respectively. For both compounds, these values of K(m) and J(max) in the colon were one to three orders of magnitude smaller than those in the small intestine, suggesting that the transport systems in the colon have by far a higher affinity and a lower transport capacity than their counterparts in the small intestine. However, it is now evident from kinetic studies that carrier-mediated transport systems for D-glucose and taurocholate are also present in the colon. It will be interesting to explore the possibility that they could be used for oral drug delivery via the colon. Their physiological roles would also be of interest.
Tomei
Shigemitsu
S
Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya 467-8603, Japan.
Torimoto
Mayumi
M
Hayashi
Yayoi
Y
Inoue
Katsuhisa
K
Yuasa
Hiroaki
H
Watanabe
Jun
J
D000818:Animals; D001692:Biological Transport; D003106:Colon / Q000378:metabolism*; D004337:Drug Carriers / Q000493:pharmacokinetics; D005947:Glucose / Q000493:pharmacokinetics*; D066298:In Vitro Techniques; D007408:Intestinal Absorption; D007421:Intestine, Small / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012256:Riboflavin / Q000493:pharmacokinetics; D013656:Taurocholic Acid / Q000493:pharmacokinetics*
D004337:Drug Carriers; D013656:Taurocholic Acid; D005947:Glucose; D012256:Riboflavin