title: Growth factor regulation of secreted matrix metalloproteinase and plasminogen activators in prostate cancer cells, normal prostate fibroblasts and normal osteoblasts.
abstract: We assessed the relative levels of secreted matrix metalloproteinases (MMPs) and plasminogen activators (PAs) in PC-3 cells, prostate fibroblasts and osteoblasts in the presence and absence of VEGF, TGF beta1 and bFGF. Fibroblasts and osteoblasts secreted more MMPs -1 and -2 than did PC-3 cells, while PC-3 s contributed the majority of PAs. MMP-1 expression was downregulated by transforming growth factor beta-1 (TGF beta1) treatment in prostate fibroblasts and upregulated by basic fibroblast growth factor (bFGF) in both stromal lines. In PC-3 cells, TGF beta1 and bFGF increased urokinase plasminogen activator secretion. TGF beta1 decreased tissue plasminogen activator secretion in all cell lines. Prostate cancer cells associated with fibroblasts or osteoblasts have a variety of MMPs and PAs to facilitate matrix degradation.
authors.affiliation: Center for Protease Research, North Dakota State University College of Pharmacy, Fargo, North Dakota 58105, USA.
authors.forename: K
authors.initials: K
authors.lastname: Forbes
authors.forename: M A
authors.initials: MA
authors.lastname: Webb
authors.forename: I
authors.initials: I
authors.lastname: Sehgal
mesh_terms: D000818:Animals; D005109:Extracellular Matrix / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005347:Fibroblasts / Q000502:physiology; D008297:Male; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D010006:Osteoblasts / Q000502:physiology*; D010960:Plasminogen Activators / Q000378:metabolism*; D011467:Prostate / Q000166:cytology*; D011471:Prostatic Neoplasms / Q000473:pathology*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D015854:Up-Regulation; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology*
chemical_list: D016212:Transforming Growth Factor beta; D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2; D010960:Plasminogen Activators; D020782:Matrix Metalloproteinases