Linkage analysis of the candidate genes of familial restless legs syndrome.
This research was performed to investigate the relationship between 16 candidate genes responsible for dopaminergic transmission or iron metabolism and familial restless legs syndrome. Genotyping was performed in a Han restless legs syndrome family using the technique of fluorescence-based genescan with the microsatellite markers selected in chromosomal regions flanking the candidate genes. Classical linkage analysis was conducted under the autosomal dominant genetic mode. Results showed that all of the LOD scores at recombination fraction 0.00 are smaller than -2.00, which indicated that these loci were not linked to familial restless legs syndrome. No linkage was found between the candidate genes and RLS in this family. Familial restless legs syndrome may be caused by another gene related to dopaminergic transmission and iron metabolism or there is new mechanism involved in this disease.
Li
Jing
J
Health Science Center, Shanghai Institutes for Biological Sciences, CAS/Shanghai Second Medical University, Shanghai 200025, China.
Hu
Lan-Dian
LD
Wang
Wei-Jun
WJ
Chen
Yu-Guang
YG
Kong
Xiang-Yin
XY
D000293:Adolescent; D000328:Adult; D002648:Child; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics; D050483:Dopamine Plasma Membrane Transport Proteins; D005192:Family Health; D005260:Female; D005293:Ferritins / Q000235:genetics; D008040:Genetic Linkage; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008126:Lod Score; D008297:Male; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins / Q000235:genetics; D018895:Microsatellite Repeats; D008875:Middle Aged; D009419:Nerve Tissue Proteins; D010375:Pedigree; D011954:Receptors, Dopamine / Q000235:genetics; D011990:Receptors, Transferrin / Q000235:genetics; D012148:Restless Legs Syndrome / Q000235:genetics* / Q000473:pathology
D050483:Dopamine Plasma Membrane Transport Proteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D011954:Receptors, Dopamine; D011990:Receptors, Transferrin; D004247:DNA; D005293:Ferritins