Neuraminidase inhibitors from Reynoutria elliptica.
In the course of screening neuraminidase inhibitors from herbal medicines, Reynoutria elliptica exhibited high inhibitory activity. Four active compounds were isolated from the ethyl acetate soluble fraction by consecutive purification using sillica gel, Sephadex LH-20 chromatography, and recrystallization. The chemical structures of these compounds were identified as 1,3,8-trihydroxy-6-methylanthraquinone (emodin) 1,8-dihydroxy-3-methoxy-6-methylanthraquinone (emodin 3-methyl ether; physcion), 1,3,8-trihydroxy-6-hydoxymethylanthraquinone (omega-hydroxyemodin), and 3,5,4'-trihydroxystilbene (trans-resvertrol) by spectral data including MS, 1H-, and 13C-NMR. The IC50 values of emodin, emodin 3-methyl ether, omega-hydroxyemodin, and trans-resvertrol were 2.81, 74.07, 10.49, and 8.77 microM, respectively. They did not inhibit other glycosidase such as glucosidase, mannosidase, and galactosidase, indicating that they were relatively specific inhibitors of neuraminidase.
Lee
Chu-Hyun
CH
Division of Applied Biology & Chemistry, College of Agriculture & Life Sciences, Kyungpook National University, 1370, Sankyuk-Dong, Daegu 702-701, Korea.
Kim
Sang-In
SI
Lee
Kyung-Bok
KB
Yoo
Yung-Choon
YC
Ryu
Si-Young
SY
Song
Kyung-Sik
KS
D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D004642:Emodin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification*; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D009439:Neuraminidase / Q000037:antagonists & inhibitors* / Q000737:chemistry; D019664:Polygonaceae / Q000737:chemistry*; D000077185:Resveratrol; D013267:Stilbenes / Q000737:chemistry / Q000302:isolation & purification*; D013329:Structure-Activity Relationship
D004365:Drugs, Chinese Herbal; D004791:Enzyme Inhibitors; D013267:Stilbenes; D009439:Neuraminidase; D004642:Emodin; D000077185:Resveratrol