Molecular epidemiology of macrolide resistance in neonatal bloodstream isolates of group B streptococci.
Pulsed-field gel electrophoresis (PFGE) was performed on 122 neonatal bloodstream isolates of group B streptococci (GBS) to further examine the relationship between macrolide resistance and serotype V GBS (GBS-V). Over one-third (35%) of macrolide-resistant GBS belonged to a single PFGE subtype of GBS-V, which was also the most common GBS-V subtype noted in previous Centers for Disease Control and Prevention surveillance studies. Erm methylase (ermA and ermB) was the most common resistance mechanism detected, present in 12 of 20 macrolide-resistant GBS.
Diekema
Daniel J
DJ
Department of Internal Medicine, University of Iowa Colleges of Medicine and Public Health, Iowa City, Iowa 52242, USA. daniel-diekema@uiowa.edu
Andrews
Janet I
JI
Huynh
Holly
H
Rhomberg
Paul R
PR
Doktor
Stella R
SR
Beyer
Jill
J
Shortridge
Virginia D
VD
Flamm
Robert K
RK
Jones
Ronald N
RN
Pfaller
Michael A
MA
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016470:Bacteremia / Q000382:microbiology; D001426:Bacterial Proteins / Q000235:genetics; D001769:Blood / Q000382:microbiology*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D007231:Infant, Newborn; D018942:Macrolides; D008780:Methyltransferases / Q000235:genetics; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D012703:Serotyping; D013290:Streptococcal Infections / Q000382:microbiology; D013292:Streptococcus agalactiae / Q000145:classification / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D018942:Macrolides; D008780:Methyltransferases