Determination of chloramphenicol residues in shrimps by liquid chromatography-mass spectrometry.
A liquid chromatographic method with mass spectrometric detection and identification (LC-MS) is presented for the determination of chloramphenicol (CAP) in shrimp tissues. Homogenized shrimp samples were extracted with phosphate buffer (pH 7.0). Clean-up was carried out on a C(18) SPE cartridge. Chloramphenicol was determined by LC-MS-ESI in negative mode. The column used was a Symmetry Shield with a mixture of acetonitrile-water (25:75) as mobile phase. Shrimp samples were fortified at CAP levels between 0.2 and 50 ng g(-1) with 5D-CAP as internal standard. At these levels, accuracies lay between 101 and 110% and between-day reproducibilities were lower than 7.1%, expressed as the variation coefficient of the mean. Limit of decision (CCalpha) was 0.02 ng g(-1). Limit of quantitation (LOQ) was 0.2 ng g(-1).
Ramos
M
M
Unidad de Residuos Zoosanitarios, Centro Nacional de Alimentación, Agencia Española de Seguridad Alimentaria, Ctra. de Pozuelo Km 2, Majadahonda, Madrid, Spain.
Muñoz
P
P
Aranda
A
A
Rodriguez
I
I
Diaz
R
R
Blanca
J
J
D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis*; D002701:Chloramphenicol / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D003445:Crustacea / Q000737:chemistry*; D004350:Drug Residues / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D000890:Anti-Infective Agents; D002701:Chloramphenicol