Effect of sodium tetraborate (borax) on the thermal properties of frozen aqueous sugar and polyol solutions.
The effect of sodium tetraborate (Na(2)B(4)O(7), borax) on the thermal property of frozen aqueous sugar and polyol solutions was studied through thermal analysis. Addition of borax raised the thermal transition temperature (glass transition temperature of maximally freeze-concentrated solutes; T(g)') of frozen sucrose solutions depending on the borax/sucrose concentration ratios. Changes in the T(g)' of frozen mono- and disaccharide solutions suggested various forms of complexes, including those of a borate ion and two saccharide molecules. Borax exerted the maximum effect to raise the oligosaccharide and dextran T(g)'s at borax/saccharide molar ratios of approximately 1-2 (maltose and maltooligosaccharides), 2 (dextran 1060), 5 (dextran 4900), and 10 (dextran 10200). Further addition of borax lowered T(g)'s of the saccharide solutions. Borax also raised T(g) and T(g)' temperatures of frozen aqueous glycerol solutions. The decreased solute mobility in frozen solutions by the borate-polyol complexes suggested higher collapse temperature in the freeze-drying process and improved stability of biological systems in frozen solutions.
Izutsu
Ken-ichi
K
National Institute of Health Sciences, Japan. izutsu@nihs.go.jp
Rimando
Annie
A
Aoyagi
Nobuo
N
Kojima
Shigeo
S
D001881:Borates / Q000737:chemistry*; D002152:Calorimetry, Differential Scanning; D003470:Culture Media / Q000737:chemistry; D003911:Dextrans / Q000737:chemistry*; D004355:Drug Stability; D005612:Freeze Drying; D008970:Molecular Weight; D009844:Oligosaccharides / Q000737:chemistry*; D012996:Solutions
D001881:Borates; D003470:Culture Media; D003911:Dextrans; D009844:Oligosaccharides; D012996:Solutions; C018851:borax