Tyrosine kinase is involved in hemin-induced pyresis.
To investigate the mechanisms involved in hemin-induced febrile response, the rectal temperature of rats were measured after intracerebroventricular (i.c.v.) injections of hemin, with or without antagonists. Hemin (10 microg) elicited a significant febrile response, which lasted from 30 min, to more than 6 h, after its administration, but this was not the case with biliverdin (i.c.v.) and bilirubin (i.c.v.). The hemin-induced febrile response was significantly inhibited by pretreatment with an inhibitor of tyrosine kinase (genistein), but not by pretreatment with an inhibitor of protein kinase C (chelerythrine) and a scavenger of iron (deferoxamine). These results suggest that tyrosine kinase is involved in the hemin-induced febrile response.
Lee
Sang-Ho
SH
Lab. of Pharmacology, College of Pharmacy, Sungkyunkwan University, Suwon 440-746, Korea.
Jang
Choon-Gon
CG
Lee
Seok-Yong
SY
D000470:Alkaloids; D000818:Animals; D053119:Benzophenanthridines; D001831:Body Temperature / Q000187:drug effects; D003676:Deferoxamine / Q000008:administration & dosage / Q000494:pharmacology; D005334:Fever / Q000139:chemically induced / Q000201:enzymology / Q000378:metabolism*; D019833:Genistein / Q000008:administration & dosage / Q000494:pharmacology; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism; D006427:Hemin / Q000008:administration & dosage / Q000378:metabolism*; D007276:Injections, Intraventricular; D007502:Iron Chelating Agents / Q000008:administration & dosage / Q000494:pharmacology; D008297:Male; D010617:Phenanthridines / Q000008:administration & dosage / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011749:Pyrogens / Q000008:administration & dosage / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000470:Alkaloids; D053119:Benzophenanthridines; D007502:Iron Chelating Agents; D010617:Phenanthridines; D011749:Pyrogens; D006427:Hemin; D019833:Genistein; C016299:chelerythrine; D006419:Heme Oxygenase (Decyclizing); D011505:Protein-Tyrosine Kinases; D011493:Protein Kinase C; D003676:Deferoxamine