Transepithelial transport of ferulic acid by monocarboxylic acid transporter in Caco-2 cell monolayers.
Our previous study (Biosci. Biotechnol. Biochem., 66, 2449-2457 (2002)), suggested that ferulic acid was transported via a monocarboxylic acid transporter (MCT). Transepithelial transport of ferulic acid was examined in this study by directly measuring the rate of its transport across Caco-2 cell monolayers. Ferulic acid transport was dependent on pH, and in a vectorical way in the apical-basolateral direction. The permeation of ferulic acid was concentration-dependent and saturable; the Michaelis constant was 16.2 mM and the maximum velocity was 220.4 nmol min-1 (mg protein)-1. Various substrates for MCTs, such as benzoic acid and acetic acid, strongly inhibited the permeation of ferulic acid, demonstrating that ferulic acid is obviously transported by MCT. Antioxidative phenolic acid compounds from dietary sources like ferulic acid would be recognized and transported by MCT by intestinal absorption.
Konishi
Yutaka
Y
Applied Bioresearch Center, Research & Development Dept., Kirin Brewery Co., Ltd., 3 Miyaharacho, Takasaki-shi, Gunma 370-1295, Japan. konishiy@kirin.co.jp
Shimizu
Makoto
M
D000975:Antioxidants / Q000378:metabolism / Q000493:pharmacokinetics; D001692:Biological Transport; D016764:Cell Polarity; D003110:Colonic Neoplasms / Q000473:pathology; D003373:Coumaric Acids / Q000378:metabolism* / Q000493:pharmacokinetics; D004848:Epithelium / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007408:Intestinal Absorption; D007700:Kinetics; D027501:Monocarboxylic Acid Transporters / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D000975:Antioxidants; D003373:Coumaric Acids; D027501:Monocarboxylic Acid Transporters; C004999:ferulic acid