title: Osteoblasts response to allogenic and xenogenic solvent dehydrated cancellous bone in vitro.
abstract: The present in vitro study investigates the cellular interaction of primary human osteoblasts with human and bovine solvent dehydrated cancellous bone (SDCB) discs. These are bio-implants from solvent dehydrated, gamma-irradiated preserved human and bovine cancellous bone, pre-treated to remove all cells, genetic components and water soluble proteins. Primary human osteoblasts were harvested from cancellous chips of trauma patients undergoing osteosynthesis with bone grafting from the iliac crest. All patients provided informed consent. The present investigation tested proliferation, synthesis of phenotypic marker, and morphology of primary cultured human osteoblasts on SDCB in vitro. The total protein and collagen type 1 content could not be revealed, due to the inherent naturally occurring protein content in these two bio-implants. In conclusion, our in vitro results suggest that SDCB may be a suitable bone substitute which provides a well structured and biocompatible scaffold for ingrowing human osteoblasts.
authors.affiliation: Surgical Research Division E-Lab 36, Department of Surgery, University Hospital Zurich, Raemi str. 100, CH-8091 Zurich, Switzerland. omana.trentz@chi.usz.ch
authors.forename: Omana A
authors.initials: OA
authors.lastname: Trentz
authors.forename: Simon P
authors.initials: SP
authors.lastname: Hoerstrup
authors.forename: Li K
authors.initials: LK
authors.lastname: Sun
authors.forename: Lukas
authors.initials: L
authors.lastname: Bestmann
authors.forename: Andreas
authors.initials: A
authors.lastname: Platz
authors.forename: Otmar L
authors.initials: OL
authors.lastname: Trentz
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D018786:Bone Substitutes; D001842:Bone and Bones; D002417:Cattle; D048429:Cell Size; D002478:Cells, Cultured; D006801:Humans; D008297:Male; D008422:Materials Testing; D010006:Osteoblasts / Q000502:physiology* / Q000648:ultrastructure; D015675:Osteocalcin / Q000378:metabolism; D019736:Prostheses and Implants; D012997:Solvents; D014183:Transplantation, Heterologous; D014184:Transplantation, Homologous; D014867:Water
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D012997:Solvents; D014867:Water; D015675:Osteocalcin