A study about the effect of vitamin E on hyperlipidemia and atherosclerotic lesions in New Zealand white rabbits fed with a 1% cholesterol rich diet.
The purpose of this study was to determine whether the administration of Vitamin E (200 mg/day) for 4 weeks could decrease hypercholesterolemia and inhibit atherosclerosis in male hypercholesterolemic New Zealand White rabbits. Plasmatic Total Cholesterol (TC) and LDL-Cholesterol (LDLc) were determined by enzymatic methods and Vitamin E was determined in plasma by HPLC at weeks 0, 4 and 8. A histopathological study was carried out at week 8 (w 8), using a hematoxylin-eosin method. Forty rabbits were divided randomly into 5 groups and fed different diets. These diets included a Normal diet, a 1% Cholesterol rich diet, a Normal diet + Vitamin E and 1% Cholesterol rich diet + Vitamin E. The findings did not show a reduction of Total Cholesterol and LDLc in the groups of rabbits that received Vitamin E (III and IV) at weeks 4 and 8. At the end of the experiment, all the animals were deeply anesthetized with hydrochloride ketamina (60 mg/kg body wt) in order to carry out a histopathological study. Regarding the atherosclerotic lesions, Vitamin E did not induce inhibition of the atherosclerotic plaque development or any modification in the lesion type induced by the hypercholesterolemic diet. In conclusion, the results suggest that additional studies need to be carried out with higher doses and/or treatments for a longer period in order to clarify in detail whether Vitamin E really has a hypocholesterolemic effect and inhibits atherosclerosis.
Torres
Marysabel
M
Department of Pharmacology, School of Medicine, University of Carabobo, Valencia, Venezuela. mtorresr@uc.edu.ve
Márquez
Mercedes
M
Sutil
Rosalía
R
Carrizales
Maira
M
Leal
María
M
Reigosa
Aldo
A
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D002784:Cholesterol / Q000097:blood; D002791:Cholesterol, Dietary / Q000008:administration & dosage*; D008078:Cholesterol, LDL / Q000097:blood / Q000187:drug effects; D003324:Coronary Artery Disease / Q000188:drug therapy* / Q000209:etiology; D003331:Coronary Vessels / Q000187:drug effects / Q000473:pathology; D006949:Hyperlipidemias / Q000188:drug therapy* / Q000209:etiology; D008297:Male; D011817:Rabbits; D013997:Time Factors; D014810:Vitamin E / Q000097:blood / Q000494:pharmacology* / Q000627:therapeutic use
D000975:Antioxidants; D002791:Cholesterol, Dietary; D008078:Cholesterol, LDL; D014810:Vitamin E; D002784:Cholesterol