title: Determination of aromatic amine mutagens, PBTA-1 and PBTA-2, in river water by solid-phase extraction followed by liquid chromatography-tandem mass spectrometry.
abstract: We describe a novel method for the determination of two kinds of aromatic amine mutagens, 2-[2-(acetylamino)-4-[bis(2-methoxyethyl)-amino]-5-amino-7-bromo-4-chloro-2H-benzotriazole (PBTA-1) and 2-[2-(acetylamino)-4-[bis(2-cyanoethyl)-ethylamino]-5-amino-7-bromo-4-chloro-2H-benzotriazole (PBTA-2), in river water based on liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS-MS). A solid-phase extraction procedure was used for the extraction of PBTA-1 and PBTA-2 from river water. The procedure was rapid and the relative standard deviations were below 4%. The detection limits of PBTA-1 and PBTA-2 in river water using the proposed method were found to be 1 and 2 ng/l, respectively. The compounds were detected by this method in river water taken from two sites in the Yodo River system at the ng/l level.
authors.affiliation: Osaka City Institute of Public Health and Environmental Sciences, 8-34, Tojo-cho, Tennoji-ku, Osaka 543-0026 Japan. moriwaki@oct.zaq.ne.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Moriwaki
authors.forename: Hiroya
authors.initials: H
authors.lastname: Harino
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Hashimoto
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Arakawa
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ohe
authors.forename: Taro
authors.initials: T
authors.lastname: Yoshikura
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization; D014230:Triazoles / Q000032:analysis*
chemical_list: C115463:2-(2-(acetylamino)-4-(N-(2-cyanoethyl)ethylamino)-5-methoxyphenyl)-5-amino-7-bromo-4-chloro-2H-benzotriazole; C112866:2-(2-(acetylamino)-4-(bis(2-methoxyethyl)amino)-5-methoxyphenyl)-5-amino-7-bromo-4-chloro-2H-benzotriazole; D014230:Triazoles