Gold electrodes wired for coupling with the deeply buried active site of Arthrobacter globiformis amine oxidase.
Diethylaniline-terminated oligo(phenyl-ethynyl)-thiol (DEA-OPE-SH) wires on Au-bead electrodes facilitate electron tunneling to and from the deeply buried topaquinone (TPQ) cofactor in Arthrobacter globiformis amine oxidase (AGAO). Reversible cyclic voltammograms were observed when AGAO was adsorbed onto this DEA-OPE-SAu surface: the 2e-/2H+ reduction potential is -140 mV versus SCE.
Hess
Corinna R
CR
Beckman Institute, California Institute of Technology, Pasadena, California 91125, USA.
Juda
Gregory A
GA
Dooley
David M
DM
Amii
Ricky N
RN
Hill
Michael G
MG
Winkler
Jay R
JR
Gray
Harry B
HB
D006631:Amine Oxidase (Copper-Containing) / Q000737:chemistry* / Q000378:metabolism; D001173:Arthrobacter / Q000201:enzymology*; D001665:Binding Sites; D004295:Dihydroxyphenylalanine / Q000031:analogs & derivatives* / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D008958:Models, Molecular; D010084:Oxidation-Reduction
D004295:Dihydroxyphenylalanine; C067626:6-hydroxydopa quinone; D006046:Gold; D006631:Amine Oxidase (Copper-Containing)