Identification of Arabidopsis rat mutants.
Limited knowledge currently exists regarding the roles of plant genes and proteins in the Agrobacterium tumefaciens-mediated transformation process. To understand the host contribution to transformation, we carried out root-based transformation assays to identify Arabidopsis mutants that are resistant to Agrobacterium transformation (rat mutants). To date, we have identified 126 rat mutants by screening libraries of T-DNA insertion mutants and by using various "reverse genetic" approaches. These mutants disrupt expression of genes of numerous categories, including chromatin structural and remodeling genes, and genes encoding proteins implicated in nuclear targeting, cell wall structure and metabolism, cytoskeleton structure and function, and signal transduction. Here, we present an update on the identification and characterization of these rat mutants.
Zhu
Yanmin
Y
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-1392, USA.
Nam
Jaesung
J
Humara
Jaime M
JM
Mysore
Kirankumar S
KS
Lee
Lan-Ying
LY
Cao
Hongbin
H
Valentine
Lisa
L
Li
Jingling
J
Kaiser
Anthony D
AD
Kopecky
Andrea L
AL
Hwang
Hau-Hsuan
HH
Bhattacharjee
Saikat
S
Rao
Praveen K
PK
Tzfira
Tzvi
T
Rajagopal
Jyothi
J
Yi
HoChul
H
Veena
Yadav
Badam S
BS
Crane
Yan M
YM
Lin
Kui
K
Larcher
Yves
Y
Gelvin
Matthew J K
MJ
Knue
Marnie
M
Ramos
Cynthia
C
Zhao
Xiaowen
X
Davis
Susan J
SJ
Kim
Sang-Ic
SI
Ranjith-Kumar
C T
CT
Choi
Yoo-Jin
YJ
Hallan
Vipin K
VK
Chattopadhyay
Sudip
S
Sui
Xiangzhen
X
Ziemienowicz
Alicja
A
Matthysse
Ann G
AG
Citovsky
Vitaly
V
Hohn
Barbara
B
Gelvin
Stanton B
SB
D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000502:physiology; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics; D001483:Base Sequence; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D004798:Enzymes / Q000235:genetics; D009154:Mutation; D018517:Plant Roots / Q000378:metabolism; D016133:Polymerase Chain Reaction; D016372:RNA, Antisense / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics
D029681:Arabidopsis Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D004798:Enzymes; D016372:RNA, Antisense; D034741:RNA, Small Interfering; C036483:T-DNA