[Establishment of human multidrug-resistant lung carcinoma cell line (D6/MVP)].
OBJECTIVE
To establish human multidrug-resistant lung carcinoma cell line (D6/MVP) with its characteristics studied.


METHODS
Intermittent administration of high-dose MMC, VDS and DDP (MVP) was used to induce human lung carcinoma cell line (D6) to a multidrug-resistant variety (D6/MVP). MTT assay was used to study the multidrug resistance of D6/MVP to multianticarcinogen. Flow cytometry was used to study the cell cycle distribution and the expression of P-gp, multidrug resistance-associated protein (MRP) and GSH/GST.


RESULTS
1. D6/MVP was resistant to many anti-tumor agents, with the IC(50) 13.3 times higher and the drug resistance 2 - 6 times higher than D6, 2. The multiplication time of D6/MVP was prolonged and the cell number of S-phase decreased while that of G1- and G(2)-phase increased and 3. The expression of P-gp and MRP was enhanced significantly (96.2% vs 51.7%), but the expression of GSH/GST kept stable.


CONCLUSIONS
D6/MVP is a multidrug-resistant cell line possessing the basic characteristics of drug-resistance.
Ma
Sheng-lin
SL
Department of Medical Oncology, Zhejiang Cancer Hospital, Hangzhou 310022, China.
Feng
Jian-guo
JG
Gu
Lin-hui
LH
Ling
Yu-tian
YT
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000032:analysis; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000008:administration & dosage / Q000627:therapeutic use; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000008:administration & dosage; D018432:Drug Resistance, Multiple; D005434:Flow Cytometry; D005978:Glutathione / Q000032:analysis; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000473:pathology; D008937:Mitomycins / Q000008:administration & dosage; D014747:Vinblastine / Q000008:administration & dosage
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D008937:Mitomycins; D014747:Vinblastine; D005982:Glutathione Transferase; D005978:Glutathione; D002945:Cisplatin