The intraflagellar transport machinery of Chlamydomonas reinhardtii.
First discovered in the green alga, Chlamydomonas, intraflagellar transport (IFT) is the bidirectional movement of protein particles along the length of eukaryotic cilia and flagella. Composed of approximately 16 different proteins, IFT particles are moved out to the distal tip of the organelle by kinesin-II and are brought back to the cell body by cytoplasmic dynein 1b. Mutant analysis of the IFT motor and particle proteins using diverse organisms has revealed a conserved and essential role for IFT in the assembly and maintenance of cilia and flagella. IFT is thought to mediate this assembly through the delivery of axonemal precursors out to the distal tip of the growing organelle. Consistent with this model, the IFT particle proteins are rich in protein-protein binding motifs, suggesting that the particles may act as scaffolds for the binding of multiple cargoes. With most of the IFT proteins now identified at the level of the gene, this review will briefly examine both the structure and function of the IFT machinery of Chlamydomonas reinhardtii.
Cole
Douglas G
DG
Department of Microbiology, Molecular Biology and Biochemistry, University of Idaho, Moscow, Idaho, 83844-3052, USA. dcole@uidaho.edu
D020418:Algal Proteins / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D016825:Chlamydomonas reinhardtii / Q000166:cytology* / Q000378:metabolism*; D005407:Flagella / Q000378:metabolism*; D008958:Models, Molecular; D020409:Molecular Motor Proteins / Q000235:genetics / Q000378:metabolism
D020418:Algal Proteins; D020409:Molecular Motor Proteins