title: Exacerbation of experimental autoimmune encephalomyelitis in rodents infected with murine gammaherpesvirus-68.
abstract: Viral infections have long been suspected to play a role in the pathogenesis of multiple sclerosis. In the present study, two different rodent models of experimental autoimmune encephalomyelitis (EAE) were used to demonstrate the ability of murine gammaherpesvirus-68 (gammaHV-68) to exacerbate development of neurological symptoms. SJL mice received UV-inactivated gammaHV-68 or intranasalgammaHV-68, followed by immunization against proteolipid-protein peptide 139-151. Infected mice became moribund within 10 days post-immunization, whereas mice exposed to UV-inactivated gammaHV-68 recovered. In the second model, Lewis rats were exposed to UV-inactivated gammaHV-68 or to gammaHV-68, followed by passive transfer of encephalitogenic T lymphocytes specific for myelin basic protein. Consistently, infected rats had higher clinical scores, and this result was observed during acute or latent gammaHV-68 infection. It is unlikely that this gammaHV-68-induced exacerbation was due to significant viral replication within the central nervous system since nested PCR, viral plaque assays, and infectious-centers assays demonstrated no detectable virus in spinal cords or brains of infected rodents undergoing EAE. Taken together, these studies demonstrate increased clinical symptoms of EAE in rodents infected by a gammaherpesvirus that has a limited ability to invade the central nervous system.
authors.affiliation: Department of Biology, The University of North Carolina at Charlotte, Charlotte, NC 28223, USA.
authors.forename: James W
authors.initials: JW
authors.lastname: Peacock
authors.forename: Sherine F
authors.initials: SF
authors.lastname: Elsawa
authors.forename: Cynthia C
authors.initials: CC
authors.lastname: Petty
authors.forename: William F
authors.initials: WF
authors.lastname: Hickey
authors.forename: Kenneth L
authors.initials: KL
authors.lastname: Bost
mesh_terms: D000818:Animals; D004279:DNA, Viral / Q000378:metabolism; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000378:metabolism* / Q000503:physiopathology; D006566:Herpesviridae Infections / Q000378:metabolism*; D051379:Mice; D051381:Rats; D018144:Rhadinovirus / Q000472:pathogenicity*; D014412:Tumor Virus Infections / Q000378:metabolism*
chemical_list: D004279:DNA, Viral