title: [Effect of triglycerides on rat islet cell function in vitro].
abstract: OBJECTIVE
  To study the effect of triglycerides (TGs) on the function of in vitro cultured rat islet cells.
  
  
  METHODS
  SD rat islet cells were isolated for monolayer cell culture and treated with TGs at different concentrations (0, 0.5, 2.5, 5.0, 10.0 mmol/L) for 72 h. Insulin level in the cell culture media was measured after glucose loading test, and the deposition of fat droplets in the islet cells observed by oil red O-staining.
  
  
  RESULTS
  No obvious effect was observed after a 72-h incubation of the islet cells with TGs at low glucose level (2.8 mmol/L), while with the stimulation of high-level glucose (27.8 mmol/L), the insulin secretion was significantly inhibited by TGs of higher concentrations (5.0, 10.0 mmol/L). The deposition of fat droplets was found in the islet cells after incubation with TGs, with TGs of higher concentrations.
  
  
  CONCLUSIONS
  TGs induces deposition of fat droplets in islet cells in vitro, and may inhibit the insulin-secreting function of the cells.
authors.affiliation: Department of Endocrinology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
authors.forename: Zi-ying
authors.initials: ZY
authors.lastname: Zhu
authors.forename: Yao-ming
authors.initials: YM
authors.lastname: Xue
authors.forename: Yi-zhen
authors.initials: YZ
authors.lastname: Jiang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D050356:Lipid Metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014280:Triglycerides / Q000494:pharmacology*
chemical_list: D007328:Insulin; D014280:Triglycerides