title: A simple pump-assisted method for collecting live, undamaged Psoroptes ovis from sheep using circulating saline.
abstract: The availability of large numbers of undamaged sheep scab mites, Psoroptes ovis, would be beneficial for discovery screening and development trials. There are several reported procedures for removing scab mites from sheep but they have limitations. To overcome this, a simple but versatile method employing the use of pumped saline was developed to remove all stages of the P. ovis mite from sheep. The method takes no more than 2 min to remove mites from the selected site with relative ease and is not affected by the condition of the fleece or lesion. The number of mites removed with the new method was 5-10 times more than detected by visual examination. These mites were undamaged and survived off-host for up to 16 days. The robust, portable equipment is easy to use under field conditions, making this method suitable for use as a diagnostic tool for early detection and monitoring of scab mites thus providing opportunities for development of novel alternative control strategies.
authors.affiliation: Central Science Laboratory, Department for Environment, Food and Rural Affairs, Sand Hutton, York Y041 1LZ, UK. b.thind@csl.gov.uk
authors.forename: B B
authors.initials: BB
authors.lastname: Thind
authors.forename: H L
authors.initials: HL
authors.lastname: Ford
mesh_terms: D000818:Animals; D005260:Female; D008297:Male; D008853:Microscopy / Q000379:methods / Q000662:veterinary; D008924:Mite Infestations / Q000469:parasitology / Q000662:veterinary*; D040042:Psoroptidae / Q000502:physiology*; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology*; D013048:Specimen Handling / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*; D014935:Wool / Q000469:parasitology