Purification of parietal and chief cells from the gastric mucosa.
Acid-secreting parietal cells from the gastric mucosa are widely studied as a model in studies on ion transport. A discontinuous gradient of iodixanol has been found to be superior to earlier protocols using Nycodenz and this method, which removes a significant amount of contaminating cells and mucus is a very useful prelude to further purification by elutriation.
Graham
John M
JM
School of Biomolecular Sciences, Liverpool John Moores University. john@jgrescon.fsbusiness.co.uk
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient / Q000379:methods*; D019872:Chief Cells, Gastric / Q000166:cytology*; D005753:Gastric Mucosa / Q000166:cytology*; D010295:Parietal Cells, Gastric / Q000166:cytology*; D011817:Rabbits; D051381:Rats