title: Antibody W7C5 defines a CD109 epitope expressed on CD34+ and CD34- hematopoietic and mesenchymal stem cell subsets.
abstract: The cell surface antigen recognized by monoclonal antibody W7C5 is expressed at low levels on human CD34(+) and CD34(-) bone marrow stem cell populations but at high levels on fetal liver CD34(+) cells. To identify the recognized antigen, we performed partial amino acid sequence and MALDI analysis of purified W7C5 antigens. The analyses revealed that MoAb W7C5 detects CD109, a recently cloned glycophosphatidylinositol-linked glycoprotein. The specificity of MoAb W7C5 was further confirmed by the selective recognition of CHO cells transfected with human CD109 cDNA.
authors.affiliation: University of Tübingen, Department of Internal Medicine II, Division of Hematology, Immunology, and Oncology, Tübingen, Germany.
authors.forename: Christina
authors.initials: C
authors.lastname: Giesert
authors.forename: Anke
authors.initials: A
authors.lastname: Marxer
authors.forename: D Robert
authors.initials: DR
authors.lastname: Sutherland
authors.forename: Andre C
authors.initials: AC
authors.lastname: Schuh
authors.forename: Lothar
authors.initials: L
authors.lastname: Kanz
authors.forename: Hans-Jorg
authors.initials: HJ
authors.lastname: Buhring
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000737:chemistry* / Q000378:metabolism*; D018952:Antigens, CD34 / Q000378:metabolism*; D000954:Antigens, Surface / Q000737:chemistry* / Q000378:metabolism*; D000939:Epitopes; D005434:Flow Cytometry; D058851:GPI-Linked Proteins; D006412:Hematopoietic Stem Cells / Q000145:classification*; D006801:Humans; D008648:Mesoderm / Q000166:cytology*; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D017386:Sequence Homology, Amino Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018952:Antigens, CD34; D000954:Antigens, Surface; C120523:CD109 protein, human; D000939:Epitopes; D058851:GPI-Linked Proteins; D009363:Neoplasm Proteins; C432084:W7C5 antigen, mouse