title: Analysis of pentacyclic triterpenic acids from frankincense gum resins and related phytopharmaceuticals by high-performance liquid chromatography. Identification of lupeolic acid, a novel pentacyclic triterpene.
abstract: An HPLC gradient method with photodiode array detection was developed for the simultaneous analysis of 12 different pentacyclic triterpenic acids in Indian and African frankincense gum resins as well as in related phytopharmaceuticals. The triterpenic acids were obtained by an exhaustive extraction procedure. Identification of the compounds was based on retention times, UV-spectra and add on technique with standards isolated from African frankincense. The method allows differentiation of frankincense of different origin and standardization of frankincense-based phytopharmaceuticals. Further, this is the first report identifying a novel pentacyclic triterpene, lupeolic acid, as a constituent of frankincense gum resins.
authors.affiliation: Department of Pharmacology of Natural Products and Clinical Pharmacology, University of Ulm, Helmholtzstrasse 20, D-89081 Ulm, Germany.
authors.forename: Berthold
authors.initials: B
authors.lastname: Büchele
authors.forename: Waltraud
authors.initials: W
authors.lastname: Zugmaier
authors.forename: Thomas
authors.initials: T
authors.lastname: Simmet
mesh_terms: D029743:Boswellia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010944:Plants / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D014315:Triterpenes / Q000032:analysis*; D000094042:Lupanes
chemical_list: D014315:Triterpenes; C482140:lupeolic acid; D000094042:Lupanes