Progress on isolation and short-term ex-vivo culture of highly purified non-apoptotic human intestinal epithelial cells (IEC).
Intestinal epithelial cells (IEC) form the largest surface of the human body and are of pivotal importance to digest and absorb nutrients. Furthermore these cells play a critical role shielding the organism against microorganisms and toxins present in the intestinal lumen. It is therefore not surprising that a large group of researchers take great interest in the study of these cells. However, to date it is a challenge to purify viable primary human intestinal epithelial cells and it has been even more fastidious to maintain IEC in culture ex-vivo as IEC undergo apoptosis within hours due to loss of cell anchorage ('anoikis') following the isolation process. Over recent years the authors aimed to continuously improve the isolation technique for primary IEC, allowing a simple, effective and rapid isolation of highly purified non-apoptotic human IEC. In this study the newly improved method is presented and applied to establish ex-vivo cultures of highly purified, fully viable primary IEC displaying important functional properties, making these cells amenable for ex-vivo research on primary human intestinal epithelial cells.
Grossmann
Johannes
J
Department of Medicine I, University of Regensburg, Regensburg, Germany. Johannes.Grossmann@klinik.uni-regensburg.de
Walther
Kathrin
K
Artinger
Monika
M
Kiessling
Stephan
S
Steinkamp
Martin
M
Schmautz
Wolf-Kuno
WK
Stadler
Florian
F
Bataille
Frauke
F
Schultz
Michael
M
Schölmerich
Jürgen
J
Rogler
Gerhard
G
D017209:Apoptosis / Q000502:physiology*; D015153:Blotting, Western; D002448:Cell Adhesion; D002455:Cell Division; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D005434:Flow Cytometry; D019073:HT29 Cells; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000166:cytology*; D013997:Time Factors; D019014:fas Receptor / Q000096:biosynthesis
D019014:fas Receptor; D007371:Interferon-gamma