Evaluation of the epidemiologic utility of secondary typing methods for differentiation of Mycobacterium tuberculosis isolates.
Spoligotyping and mycobacterial interspersed repetitive unit-variable-number tandem repeat analysis (MIRU-VNTR) were evaluated for the ability to differentiate 64 Mycobacterium tuberculosis isolates from 10 IS6110-defined clusters. MIRU-VNTR performed slightly better than spoligotyping in reducing the number of clustered isolates and the sizes of the clusters. All epidemiologically related isolates remained clustered by MIRU-VNTR but not by spoligotyping.
Kwara
Awewura
A
Department of Tropical Medicine, Tulane University School of Public Health and Tropical Medicine, New Orleans, Louisiana 70112, USA. Awewura_Kwara@brown.edu
Schiro
Ronald
R
Cowan
Lauren S
LS
Hyslop
Newton E
NE
Wiser
Mark F
MF
Roahen Harrison
Stephanie
S
Kissinger
Patricia
P
Diem
Lois
L
Crawford
Jack T
JT
D015373:Bacterial Typing Techniques; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D005838:Genotype; D006801:Humans; D018598:Minisatellite Repeats / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D009838:Oligodeoxyribonucleotides / Q000032:analysis; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D014376:Tuberculosis / Q000453:epidemiology* / Q000382:microbiology
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D009838:Oligodeoxyribonucleotides