title: Diagnosis of histoplasmosis by detection of the internal transcribed spacer region of fungal rRNA gene from a paraffin-embedded skin sample from a dog in Japan.
abstract: The lesions of histoplasmosis in dogs in Japan differ from those in dogs in North America. Affected dogs in Japan have had multiple granulomatous or ulcerated foci in skin or gingiva and have not had pulmonary or gastrointestinal lesions. The present report introduces a polymerase chain reaction (PCR) diagnosis of canine histoplasmosis and the characteristic of disease in Japan. The surgically removed skin ulcerate samples from a 5-years-old female Shiba-inu native to Japan without traveling out of the country were evaluated. Tissue samples had many yeast-like organisms in the macrophages. DNA was extracted from paraffin-embedded tissue samples. A nested PCR technique was applied. The detected sequence of the internal transcribed spacer of ribosomal RNA gene had 99.7% in homology with Ajellomyces capsulatus (the teleomorph of Histoplasma capsulatum). Clinical manifestations, historical background of equine epizootic lymphangitis in Japan, and a human autochthonous case of histoplasmosis farciminosi indicated that this dog might have been infected with H. capsulatum var. farciminosum as a heteroecism.
authors.affiliation: Ueda Animal Clinic, 3-16-2, Nakamura-Minami, Nerima-Ku, Tokyo 176-0025, Japan.
authors.forename: Yachiyo
authors.initials: Y
authors.lastname: Ueda
authors.forename: Ayako
authors.initials: A
authors.lastname: Sano
authors.forename: Miki
authors.initials: M
authors.lastname: Tamura
authors.forename: Tomo
authors.initials: T
authors.lastname: Inomata
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Kamei
authors.forename: Koji
authors.initials: K
authors.lastname: Yokoyama
authors.forename: Fukuko
authors.initials: F
authors.lastname: Kishi
authors.forename: Junko
authors.initials: J
authors.lastname: Ito
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Mikami
authors.forename: Makoto
authors.initials: M
authors.lastname: Miyaji
authors.forename: Kazuko
authors.initials: K
authors.lastname: Nishimura
mesh_terms: D000818:Animals; D001483:Base Sequence; D021903:DNA, Ribosomal Spacer / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D004283:Dog Diseases / Q000175:diagnosis* / Q000382:microbiology; D004285:Dogs; D005260:Female; D006658:Histoplasma / Q000235:genetics / Q000302:isolation & purification*; D006660:Histoplasmosis / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D007564:Japan; D008264:Macrophages / Q000382:microbiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D012331:RNA, Fungal / Q000235:genetics* / Q000302:isolation & purification; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D012867:Skin / Q000382:microbiology / Q000473:pathology
chemical_list: D021903:DNA, Ribosomal Spacer; D012331:RNA, Fungal; D012340:RNA, Ribosomal, 5.8S