The leading role of membrane Ca(2+)-ATPase in recovery of Ca(2+) homeostasis after glutamate shock.
Combined blockade of Na(+)/Ca(2+) exchange, Ca(2+) uptake by mitochondria and endoplasmic reticulum usually does not prevent recovery of the basal level of intracellular Ca(2+) after 1-min action of glutamate (100 microM) or K(+) (50 mM). However, replacement of Ca(2+) with Ba(2+), which cannot be transported by Ca(2+)-ATPase, considerably delayed the decrease in intracellular Ba(2+) after its rise caused by glutamate or potassium application in all examined cells, which attest to an important role of Ca(2+)-ATPase in Ca(2+) extrusion after the action of glutamate or K(+).
Storozhevykh
T P
TP
Laboratory of Membranology, Research Center of Children Health, Russian Academy of Medical Sciences, Moscow. tstor@mail.ru
Pinelis
V G
VG
Vinskaya
N P
NP
Surin
A M
AM
Khodorov
B I
BI
D000818:Animals; D000831:Animals, Newborn; D001464:Barium / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology; D018691:Excitatory Amino Acid Antagonists / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism*; D006706:Homeostasis; D008094:Lithium / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D011810:Quinoxalines / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013050:Spectrometry, Fluorescence
D018691:Excitatory Amino Acid Antagonists; D011810:Quinoxalines; C062865:2,3-dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline; D001464:Barium; D018698:Glutamic Acid; D008094:Lithium; D000252:Calcium-Transporting ATPases; D011188:Potassium; D002118:Calcium