Effects of n-3 fatty acid, fructose-1,6-diphosphate and glutamine on mucosal cell proliferation and apoptosis of small bowel graft after transplantation in rats.
OBJECTIVE
To evaluate the effects of n-3 fatty acids (n-3FA), fructose-1,6-diphosphate (FDP) and glutamine (GLN) on mucosal cell proliferation and apoptosis of small bowel graft.


METHODS
One hundred and ninety-six inbred strain Wistar rats were grouped as donors and recipients, and underwent heterotopic small bowel transplantation (SBT). n-3FA, FDP and GLN were administered via gastric tube as well as venous infusion for 10 days before and after surgery, respectively. The proliferation and apoptosis of mucosal cells were analyzed with flow cytometry and in situ cell death detection kits.


RESULTS
Apparent apoptosis and minor proliferation of mucosal cells of small bowel graft after transplantation were observed. A higher mucosal cell proliferative index and lower apoptotic index were found in all small bowel grafts after supplying with n-3FA, FDP and GLN.


CONCLUSIONS
Nutritional support with n-3FA, FDP and GLN promotes mucosal cell proliferation significantly, and prevents mucosal cell from undergoing apoptosis with different degrees. These regulatory effects on the apoptosis alter the structure and absorption function of transplanted small bowel favorably.
Wu
Xiao-Ting
XT
Department of General Surgery, West China Hospital, Sichuan University, 37 Guo Xue Rd., Chengdu 610041, Sichuan Province, China. wxt1@medmail.com.cn
Li
Jie-Shou
JS
Zhao
Xiao-Fei
XF
Li
Ning
N
Ma
Yu-Kui
YK
Zhuang
Wen
W
Zhou
Yong
Y
Yang
Gang
G
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D005635:Fructosediphosphates / Q000494:pharmacology*; D005973:Glutamine / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000473:pathology* / Q000503:physiopathology*; D007421:Intestine, Small / Q000637:transplantation*; D008297:Male; D011184:Postoperative Period; D051381:Rats; D017208:Rats, Wistar
D015525:Fatty Acids, Omega-3; D005635:Fructosediphosphates; D005973:Glutamine; C029063:fructose-1,6-diphosphate