Molecular characterization of isolates of waterborne Cryptosporidium spp. collected during an outbreak of gastroenteritis in South Burgundy, France.
In September 2001, a waterborne outbreak of gastroenteritis occurred in eastern France. Of 31 fecal samples from symptomatic individuals, 19 tested positive for Cryptosporidium with two PCRs targeting the Hsp70 and the 18S rRNA genes of CRYPTOSPORIDIUM: Sequencing of the PCR fragments produced sequences identical to that of Cryptosporidium parvum genotype 1.
Dalle
Frédéric
F
Laboratoire de Parasitologie Mycologie et Laboratoire de Microbiologie Médicale et Moléculaire (EA 562), CHU et Faculté de Médecine, Dijon, France.
Roz
Pascale
P
Dautin
Guillaume
G
Di-Palma
Marc
M
Kohli
Evelyne
E
Sire-Bidault
C
C
Fleischmann
Marie George
MG
Gallay
Anne
A
Carbonel
Sylvia
S
Bon
Fabienne
F
Tillier
Claude
C
Beaudeau
Pascal
P
Bonnin
Alain
A
D000818:Animals; D001483:Base Sequence; D003457:Cryptosporidiosis / Q000453:epidemiology / Q000469:parasitology; D016785:Cryptosporidium parvum / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000032:analysis; D004196:Disease Outbreaks; D005243:Feces / Q000469:parasitology; D005602:France; D005759:Gastroenteritis / Q000453:epidemiology* / Q000469:parasitology*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014867:Water / Q000469:parasitology*
D004275:DNA, Ribosomal; D018840:HSP70 Heat-Shock Proteins; D012337:RNA, Ribosomal, 18S; D014867:Water