title: The relative effects of supplemental dietary calcium and oxalate on urine composition and calcium oxalate relative supersaturation in healthy adult dogs.
abstract: The aim of this study was to establish the relative effects of dietary calcium and oxalate (in the form of oxalic acid) on the composition of urine produced by healthy adult Cairn Terriers and Miniature Schnauzers. A nutritionally complete dry dog food was fed to 7 dogs (4 Cairn terriers and 3 Miniature schnauzers) for 24 weeks. The dogs were fed the diet alone, or supplemented with six different combinations of dietary calcium (as carbonate and sulphate) and oxalate (as oxalic acid) commonly found in dry commercially prepared dog foods. Urine pH, volume, specific gravity, and concentrations of 12 analytes were measured for each dog; urinary relative supersaturation (RSS) with calcium oxalate (CaOx) was calculated from these values. The effects of supplemental calcium and oxalate were established using two-way analysis of variance and multiple range tests (least significant difference); P<0.05 was considered significant. The lowest level of dietary calcium and oxalate resulted in the lowest CaOx RSS. The high calcium, low oxalate diet resulted in the highest CaOx RSS, a low calcium diet with increased dietary oxalate also tended to increase CaOx RSS although results were highly variable. Urinary calcium concentration increased significantly with dietary calcium; urinary oxalate increased, although inconsistently, with dietary oxalic acid only when dietary calcium was low. Measures to reduce both calcium and oxalate should be considered when implementing dietary changes to reduce the risk of calcium oxalate formation in dogs. A reduction in dietary calcium without a concomitant decrease in dietary oxalate may increase the risk of CaOx crystallisation in susceptible dogs.
authors.affiliation: The WALTHAM Centre for Pet Nutrition, Waltham on the Wolds, Melton Mowbray, Leicestershire LE14 4RT, UK.
authors.forename: A E
authors.initials: AE
authors.lastname: Stevenson
authors.forename: W K
authors.initials: WK
authors.lastname: Hynds
authors.forename: P J
authors.initials: PJ
authors.lastname: Markwell
mesh_terms: D000818:Animals; D002136:Calcium, Dietary / Q000378:metabolism / Q000494:pharmacology* / Q000652:urine; D019587:Dietary Supplements; D004285:Dogs / Q000652:urine*; D005260:Female; D006863:Hydrogen-Ion Concentration; D008297:Male; D019815:Oxalic Acid / Q000378:metabolism / Q000494:pharmacology* / Q000652:urine; D013046:Specific Gravity; D014556:Urine / Q000737:chemistry
chemical_list: D002136:Calcium, Dietary; D019815:Oxalic Acid