[Genetic mapping of resistant gene to southern corn rust and the tagging analysis on different genetic background].
Southern corn rust (SCR) is a destructive disease in maize. The inbred line Qi319 is highly resistant to southern corn rust. SSR technique was employed to preliminary mapping of the resistance gene. Bulked segregant analysis revealed that two primers, phi 118 and phi 041, amplified polymorphic bands. SSR analysis on populations indicated the two primers were linked to the rust resistance gene, which was mapped on the short arm of chromosome 10. In addition, comparative analysis of the amplification bands among different populations revealed that the amplification products with the same primer in different populations were dissimilar. This result indicates that the genetic background may affect results of gene mapping and tagging. So, it is important to select suitable population to performing molecular marker analysis and gene mapping.
Chen
Cui-Xia
CX
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Xing
Quan-Hua
QH
Liang
Chun-Yang
CY
Yu
Yuan-Jie
YJ
Liang
Feng-Shan
FS
Wang
Hong-Gang
HG
Wang
Zhen-Lin
ZL
Wang
Bin
B
D001487:Basidiomycota / Q000254:growth & development*; D002874:Chromosome Mapping / Q000379:methods*; D032461:Chromosomes, Plant / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D007113:Immunity, Innate / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D012150:Polymorphism, Restriction Fragment Length; D003313:Zea mays / Q000235:genetics* / Q000382:microbiology
D018744:DNA, Plant