Antibacterial activity of casein-derived peptides isolated from rabbit (Oryctolagus cuniculus) milk.
Acid-precipitated rabbit 'whole casein' was digested by trypsin, chymotrypsin, pepsin, and clostripain to screen for possible peptides with antibacterial properties. The peptide fragments were separated by reversed-phase chromatography. The collected fractions were pooled and their antibacterial properties tested against Escherichia coli, Bacillus subtilis and Staphylococcus lentus. Three antibacterial peptide fragments derived from tryptic digestion of rabbit casein were isolated and identified. Their sequences were found as follows: HVEQLLR (residues 50-56 of beta-casein), ILPFIQSLFPFAER (residues 64-77 of beta-casein), and FHLGHLK (residues 19-25 of alpha(s1)-casein). The three peptides were synthesized and found to exert antibacterial effect against gram positive bacteria only. Proteolytic digestion of rabbit casein by chymotrypsin, pepsin and clostripain yielded several peptide fragments with antibacterial activity. Since antibiotic peptides can be released from casein during the digestion of milk proteins, our results suggest a possible antibacterial function of rabbit caseins. It is conceivable that antibacterial peptides can be generated by endopeptidases of the mammalian gastrointestinal tract possibly providing protection for new-born rabbits against aggression of micro-organisms.
Baranyi
Maria
M
Agricultural Biotechnology Center, Szent-Györgyi A. 4, H-2100 Gödöllo, Hungary. baranyi@abc.hu
Thomas
Ursula
U
Pellegrini
Antonio
A
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects; D002364:Caseins / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D002918:Chymotrypsin / Q000378:metabolism; D003546:Cysteine Endopeptidases / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects; D005260:Female; D008892:Milk / Q000737:chemistry*; D010434:Pepsin A / Q000378:metabolism; D010446:Peptide Fragments / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D011817:Rabbits; D013210:Staphylococcus / Q000187:drug effects; D014357:Trypsin / Q000378:metabolism
D000900:Anti-Bacterial Agents; D002364:Caseins; D010446:Peptide Fragments; D002918:Chymotrypsin; D014357:Trypsin; D003546:Cysteine Endopeptidases; C019913:clostripain; D010434:Pepsin A