title: Verification of a new gene on Saccharomyces cerevisiae chromosome III.
abstract: We present verification of the existence of a previously unannotated, intron-containing gene of 134 amino acids (predicted molecular weight approximately 15.5 kDa) located on chromosome III of Saccharomyces cerevisiae. Strains carrying a deletion of this gene, which we have called YCL012C, reveal no apparent phenotype. Orthologues of YCL012C are present in related species S. bayanus, S. paradoxus and Ashbya gossypii. Comparison with other fungal sequences reveals that orthologues of this gene are likely present in Schizosaccharomyces pombe, Neurospora crassa and Cryptococcus neoformans as well, indicating that YCL012C is a widely conserved fungal gene.
authors.affiliation: Department of Molecular Genetics and Microbiology, Center for Genome Technology, Duke University Medical Center, Durham, NC 27710, USA.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Zhang
authors.forename: Fred S
authors.initials: FS
authors.lastname: Dietrich
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal / Q000235:genetics*; D003001:Cloning, Molecular; D005800:Genes, Fungal / Q000235:genetics*; D016681:Genome, Fungal; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D012331:RNA, Fungal / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: D012331:RNA, Fungal