title: Stimulatory effect of a dietary casein phosphopeptide preparation on the mucosal IgA response of mice to orally ingested lipopolysaccharide from Salmonella typhimurium.
abstract: The effect on immunoglobulin production of a commercially available casein phosphopeptide preparation (CPP-III) consisting mainly of bovine alpha s2-casein (1-32) and beta-casein (1-28) in mice that had orally ingested lipopolysaccharide (LPS) from Salmonella typhimurium was investigated. No significant difference in body weight gain was observed between the mice fed on the CPP-III-added diet and those fed on the control diet. The mice fed on the CPP-III-added diet exhibited similar serum and intestinal IgG, IgM, and IgE responses towards LPS to those fed on the control diet. In contrast, fecal and intestinal anti-LPS IgA and total IgA in mice fed on the CPP-III-added diet were significantly higher than in those fed on the control diet. Spleen cells from mice fed on the CPP-III-added diet produced larger amounts of IgA, IL-5, and IL-6 than cells from mice fed on the control diet. These results suggest that dietary casein phosphopeptide may protect a host from invasion of the intestinal mucosa by food-born pathogenic microorganisms.
authors.affiliation: Integrated Department of Sciences of Functional Foods, Graduate School of Agriculture, Shinshu University, 8304 Minamiminowa-mura, Kamiina-gun, Nagano-ken 399-4598, Japan. otani84@gipmc.shinshu-u.ac.jp
authors.forename: Hajime
authors.initials: H
authors.lastname: Otani
authors.forename: Kouichi
authors.initials: K
authors.lastname: Nakano
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kawahara
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D001835:Body Weight; D002364:Caseins / Q000276:immunology* / Q000494:pharmacology; D019587:Dietary Supplements; D007070:Immunoglobulin A / Q000096:biosynthesis* / Q000187:drug effects; D007136:Immunoglobulins / Q000032:analysis / Q000187:drug effects; D015848:Interleukin-5 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D007413:Intestinal Mucosa / Q000276:immunology*; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000276:immunology*; D051379:Mice; D012480:Salmonella Infections / Q000517:prevention & control; D012486:Salmonella typhimurium; D013154:Spleen / Q000166:cytology / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D002364:Caseins; D007070:Immunoglobulin A; D007136:Immunoglobulins; D015848:Interleukin-5; D015850:Interleukin-6; D008070:Lipopolysaccharides