Structural and functional properties of IL-4delta2, an alternative splice variant of human IL-4.
Structural and functional properties of recombinant IL-4delta2, a naturally occurring splice variant of human IL-4 with a deletion of the loop region 22-37, have been analyzed. IL-4delta2 has alpha-helical structure and most likely preserves the "up-up-down-down" topology typical of the four-helix-bundle cytokines. IL-4delta2 interacts specifically with the alpha chain of IL-4R and competes effectively with IL-4 for the common binding sites. Thus, IL-4delta2 may act as a regulator of the cytokine net, being the natural antagonist of IL-4.
Vasiliev
Anatoly M
AM
Institute of Immunological Engineering, 142380 Lyubuchany, Moscow Region, Russia.
Vasilenko
Raisa N
RN
Kulikova
Nataly L
NL
Andreev
Sergey M
SM
Chikileva
Irina O
IO
Puchkova
Galina Yu
GY
Kosarev
Igor V
IV
Khodyakova
Anna V
AV
Khlebnikov
Valentin S
VS
Ptitsyn
Leonid R
LR
Shcherbakov
Grygory Ya
GY
Uversky
Vladimir N
VN
DuBuske
Lawrence M
LM
Abramov
Vyacheslav M
VM
D017398:Alternative Splicing; D002455:Cell Division / Q000502:physiology; D002942:Circular Dichroism; D003001:Cloning, Molecular; D003553:Cystine / Q000378:metabolism; D015847:Interleukin-4 / Q000235:genetics* / Q000378:metabolism; D008024:Ligands; D020033:Protein Isoforms / Q000235:genetics* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D017550:Spectroscopy, Fourier Transform Infrared; D013950:Thymus Gland / Q000378:metabolism
D008024:Ligands; D020033:Protein Isoforms; D015847:Interleukin-4; D003553:Cystine