Novel visible-light-induced photocurable tissue adhesive composed of multiply styrene-derivatized gelatin and poly(ethylene glycol) diacrylate.
A novel photocurable tissue adhesive glue, which is composed of styrene-derivatized (styrenated) gelatin, poly(ethylene glycol) diacrylate (PEGDA), and carboxylated camphorquinone in phosphate-buffered saline (PBS), was prepared. The prototype formulation suitable for arterial repair was determined based on the gel yield, degree of swelling, tissue adhesive strength, and breaking (or burst) strength in vitro. The formulated photocurable tissue adhesive glue with an appropriate viscosity was converted to a water-swollen gel within 1 min of visible light irradiation. The tissue adhesive glue, which was coated on a rat abdominal aorta incised with a pair of scissors, was immediately converted to a swollen gel upon subsequent irradiation with visible light, and concomitantly hemostasis was completed. Histological examination showed that the produced gel was tightly adhered to the artery shortly after photoirradiation. The gel gradually degraded with time and was completely absorbed within 4 weeks after treatment. These results indicate that the photocurable glue developed here may serve as a tissue adhesive glue applicable to vascular surgery.
Li
Cailong
C
Department of Biomedical Engineering, Graduate School of Medicine, Kyushu University, 3-1-1 Maidashi, Higashiku, Fukuoka 812-8582, Japan.
Sajiki
Toshinobu
T
Nakayama
Yasuhide
Y
Fukui
Masashi
M
Matsuda
Takehisa
T
D000818:Animals; D001012:Aorta, Abdominal / Q000601:surgery; D001673:Biodegradation, Environmental; D005780:Gelatin; D006488:Hemostasis, Surgical; D006801:Humans; D066298:In Vitro Techniques; D008027:Light; D008422:Materials Testing; D011092:Polyethylene Glycols; D051381:Rats; D020058:Styrene; D014014:Tissue Adhesives / Q000737:chemistry* / Q000378:metabolism / Q000528:radiation effects
D014014:Tissue Adhesives; C437167:poly(ethylene glycol)diacrylate; D011092:Polyethylene Glycols; D020058:Styrene; D005780:Gelatin