Solution conformation of the antibody-bound tyrosine phosphorylation site of the nicotinic acetylcholine receptor beta-subunit in its phosphorylated and nonphosphorylated states.
Phosphorylation of the acetylcholine receptor (AChR) seems to be responsible for triggering several effects including its desensitization and aggregation at the postsynaptic membrane and probably initiates a signal transduction pathway at the postsynaptic membrane. To study the structural and functional role of the tyrosine phosphorylation site of the AChR beta-subunit and contribute to the in-depth understanding of the structural basis of the ion channel function, we synthesized four peptides containing the phosphorylated and nonphosphorylated sequences (380-391) of the human and Torpedo AChR beta-subunits and studied their interaction with a monoclonal antibody (mAb 148) that is known to bind to this region and that is capable of blocking ion channel function. All four peptides were efficient inhibitors of mAb 148 binding to AChR, although the nonphosphorylated human peptide was considerably less effective than the three others. We then investigated the conformation acquired by all four peptides in their antibody-bound state, which possibly illustrates the local conformation of the corresponding sites on the intact AChR molecule. The phosphorylated human and Torpedo peptides adopted a distorted 3(10) helix conformation. The nonphosphorylated Torpedo peptide, which is also an efficient inhibitor, was also folded. In contrast, the nonphosphorylated human peptide (a less efficient inhibitor) presented an extended structure. It is concluded that the phosphorylation of the AChR at its beta-subunit Tyr site leads to a significant change in its conformation, which may affect several functions of the AChR.
Phan-Chan-Du
Angélique
A
Laboratoire de Chimie-Physique Macromoléculaire, UMR 7568 CNRS-INPL, Groupe ENSIC, 1 Rue Grandville, B.P. 451, 54001 Nancy Cedex, France.
Hemmerlin
Christine
C
Krikorian
Dimitrios
D
Sakarellos-Daitsiotis
Maria
M
Tsikaris
Vassilios
V
Sakarellos
Constantinos
C
Marinou
Martha
M
Thureau
Aurélien
A
Cung
Manh Thong
MT
Tzartos
Socrates J
SJ
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D001667:Binding, Competitive; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007473:Ion Channels / Q000037:antagonists & inhibitors; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology; D010766:Phosphorylation; D011487:Protein Conformation; D021122:Protein Subunits; D011863:Radioimmunoassay; D011978:Receptors, Nicotinic / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D012996:Solutions; D013329:Structure-Activity Relationship; D014101:Torpedo / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D007473:Ion Channels; D010455:Peptides; D021122:Protein Subunits; D011978:Receptors, Nicotinic; D012996:Solutions; D014443:Tyrosine