The detection of 20S-hydroxycholesterol in extracts of rat brains and human placenta by a gas chromatograph/mass spectrometry technique.
The presence of 20(S)-hydroxycholesterol in rat brains and human placenta has been established using the gas chromatography/mass spectrometry (GC/MS) select ion monitoring (SIM) technique. Identification was ensured by three criteria: the specific retention time when the compound emerges from the gas chromatogram and the two m/z ions (201 and 461amu) which are characteristic of its mass spectrum. The possible role of 20(S)-hydroxycholesterol in steroid hormone biosynthesis and in other biological processes is discussed.
Lin
Ying Yong
YY
Department of Obstetrics and Gynecology, The St. Luke's-Roosevelt Institute for Health Sciences, New York, NY 10019, USA.
Welch
Margaret
M
Lieberman
Seymour
S
D000818:Animals; D001923:Brain Chemistry; D002855:Chromatography, Thin Layer; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006888:Hydroxycholesterols / Q000737:chemistry / Q000302:isolation & purification*; D010920:Placenta / Q000737:chemistry*; D051381:Rats
D006888:Hydroxycholesterols; C025813:20-hydroxycholesterol