title: Hypervariable spacer regions are good sites for developing specific PCR-RFLP markers and PCR primers for screening actinorhizal symbionts.
abstract: While the ribosomal RNA like highly conserved genes are good molecular chronometers for establishing phylogenetic relationships, they can also be useful in securing the amplification of adjoining hyper-variable regions. These regions can then be used for developing specific PCR primers or PCR-RFL profiles to be used as molecular markers. We report here the use of ITS region of rrn operon of Frankia for developing PCR-RFL profiles capable of discriminating between closely related frankiae. We have also made use of the ITS1 region of the nuclear rrn operon of Alnus nepalensis (D Don) for designing a PCR primer for specific amplification of nuclear DNA of this tree.
authors.affiliation: Department of Botany, North-Eastern Hill University, Shillong 793 022, India.
authors.forename: Rajani
authors.initials: R
authors.lastname: Varehese
authors.forename: Vineeta S
authors.initials: VS
authors.lastname: Chauhan
authors.forename: Arvind K
authors.initials: AK
authors.lastname: Misra
mesh_terms: D029661:Alnus / Q000235:genetics; D022801:Complementarity Determining Regions; D004247:DNA / Q000737:chemistry / Q000235:genetics; D017931:DNA Primers / Q000494:pharmacology; D004262:DNA Restriction Enzymes / Q000494:pharmacology; D021901:DNA, Intergenic; D040161:Frankia / Q000235:genetics; D005798:Genes, Bacterial; D008957:Models, Genetic; D009876:Operon; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000737:chemistry; D012338:RNA, Ribosomal, 23S / Q000737:chemistry; D013559:Symbiosis
chemical_list: D022801:Complementarity Determining Regions; D017931:DNA Primers; D021901:DNA, Intergenic; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D004247:DNA; D004262:DNA Restriction Enzymes