title: [PKC-dependent activation of P44/42 MAPKs and HSP70 in signal transduction pathways during hepatocyte ischemic preconditioning].
abstract: OBJECTIVE
  To investigate the significance of PKC, P44/42 MAPKs and HSP70 signal transduction in IP.
  
  
  METHODS
  Through an in vivo rat liver IP model, PKC inhibitor, activator and MEK inhibitor were utilized to analyze the phosphorylation of PKC and P44/42 MAPKs. HSP expression, AST/ALT concentration, cellular structure and ultrastructure were also observed. All the data were statistically analyzed.
  
  
  RESULTS
  Compared with the control without IP, the phosphorylation of PKC was significantly increased in IP treated models and PKC activated group (P < 0.01) and P44/42 MAPKs and the expression of HSP were also obviously increased. In contrast, opposite changes were found in PKC or MEK inhibited groups, the phosphorylation PKC was decreased in PKC inhibited group (P < 0.01).
  
  
  CONCLUSIONS
  The model has shown that PKC activation plays a pivotal role in the activation of P44/42 MAPKs pathway that participates in the preservation of liver cells. At the same time, HSP expression is regulated by signals in P44/42 MAPKs pathway.
authors.affiliation: Department of transplantation surgery, Zhujiang Hospital, The first Military Medical University, Guangzhou, 510282, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Gao
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Yu-qiang
authors.initials: YQ
authors.lastname: Shan
authors.forename: Ming-xin
authors.initials: MX
authors.lastname: Pan
mesh_terms: D000818:Animals; D004789:Enzyme Activation; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D022781:Hepatocytes; D019194:Ischemic Preconditioning; D008099:Liver / Q000098:blood supply*; D008297:Male; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018840:HSP70 Heat-Shock Proteins; D011493:Protein Kinase C; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases