Isolation of Arcobacter butzleri and A. cryaerophilus in samples of meats and from meat-processing plants by a culture technique and detection by PCR.
A pilot survey of sources of contamination with arcobacters (representing a potential risk for humans) was done in a wide range of samples involved various kinds of meat (beef, pork, meat products, chilled chickens, etc.) from a retail level and domestic farming. Sanitary practices in slaughterhouses and production lines were checked in two different plants (a beef and pork production and a chicken processing plant). The method is based on a selective enrichment to isolate suspect strains, in combination with a PCR technique specific for arcobacters. The choice of a suitable enrichment broth and a plating agar was made with the use of pure bacterial strains and by means of real meat samples seeded with Arcobacter butzleri. The PCR technique was optimized to allow differentiation of a 1223 bp product, typical of the genus Arcobacter, and a product of 686 bp, specific for A. butzleri a total number of 198 samples were tested, of that 33 (17%) were found to be positive for the genus Arcobacter but only 22 (11%) for A. butzleri.
Vytrasová
J
J
Department of Biological and Biochemical Sciences, University of Pardubice, 532 10 Pardubice, Czechia. Jarmila.Vytrasova@upce.cz
Pejchalová
M
M
Harsová
K
K
Bínová
S
S
D000362:Agar; D000818:Animals; D020640:Arcobacter / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D002417:Cattle; D002645:Chickens / Q000382:microbiology; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D004866:Equipment Contamination; D005506:Food Contamination; D005525:Food-Processing Industry; D008460:Meat / Q000382:microbiology*; D008461:Meat Products / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013552:Swine / Q000382:microbiology
D003470:Culture Media; D004269:DNA, Bacterial; D000362:Agar