MR microscopy of magnetically labeled neurospheres transplanted into the Lewis EAE rat brain.
Stem cell transplantation is being explored as a new paradigm for the treatment of demyelinating diseases. Magnetically labeled multipotential neural precursor cells were transplanted into the ventricles of rats with acute experimental allergic encephalomyelitis (EAE) and high-resolution (microscopic) MR images were obtained ex vivo. Migration patterns of live cells into periventricular white matter structures could be easily visualized, with a good correlation of the corresponding histopathology. The present results confirm that MR cell tracking can be used to guide the development of successful transplantation protocols.
Bulte
Jeff W M
JW
Department of Radiology and Radiological Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205-2195, USA. jwmbulte@mri.jhu.edu
Ben-Hur
Tamir
T
Miller
Bradley R
BR
Mizrachi-Kol
Rachel
R
Einstein
Ophira
O
Reinhartz
Etti
E
Zywicke
Holly A
HA
Douglas
Trevor
T
Frank
Joseph A
JA
D000818:Animals; D001921:Brain / Q000473:pathology* / Q000601:surgery; D002465:Cell Movement; D003287:Contrast Media; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000473:pathology* / Q000503:physiopathology / Q000601:surgery*; D005260:Female; D052203:Ferrosoferric Oxide; D007501:Iron; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation* / Q000379:methods*; D008853:Microscopy / Q000295:instrumentation / Q000379:methods; D008863:Microspheres; D009474:Neurons / Q000473:pathology; D010087:Oxides; D051381:Rats; D011917:Rats, Inbred Lew; D013194:Staining and Labeling / Q000295:instrumentation / Q000379:methods; D033581:Stem Cell Transplantation / Q000295:instrumentation / Q000379:methods*; D013234:Stem Cells / Q000473:pathology*
D003287:Contrast Media; D010087:Oxides; D007501:Iron; D052203:Ferrosoferric Oxide