title: Dietary arachidonic acid suppresses bone turnover in contrast to low dosage exogenous prostaglandin E(2) that elevates bone formation in the piglet.
abstract: This study was designed to compare the effects of dietary arachidonic acid (AA) versus prostaglandin E(2) (PGE(2)) on bone cell metabolism and bone mass. Twenty-eight piglets from 7 litters were randomized to 1 of 4 treatments for 15 days: fatty acid supplemented formula (FA: 0.8% of total fatty acids as AA and 0.1% of total fatty acids as DHA)+PGE(2) injections (0.1mg/kg/day), FA+saline injections, standard formula (STD: n-6:n-3 of 8:1) + PGE(2) injections or STD+saline injections. PGE(2) resulted in elevated osteoblast activity as indicated by plasma osteocalcin and also reduced urinary calcium excretion. Dietary FA resulted in reduced bone resorption as indicated by urinary N-telopeptide and reduced bone PGE(2). Both PGE(2) and FA treatments independently lead to elevated femur mineral content, but the combined treatment caused a reduction. Thus the mechanisms by which PGE(2) and FA lead to enhanced bone mass are distinct.
authors.affiliation: Department of Human Nutritional Sciences, University of Manitoba, H513 Duff Roblin Building, Winnipeg, Manitoba, Canada R3T 2N2.
authors.forename: Vienna D
authors.initials: VD
authors.lastname: Lucia
authors.forename: Shirley C
authors.initials: SC
authors.lastname: Fitzpatrick-Wong
authors.forename: Hope A
authors.initials: HA
authors.lastname: Weiler
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology*; D001842:Bone and Bones / Q000187:drug effects* / Q000378:metabolism*; D004032:Diet; D004041:Dietary Fats / Q000494:pharmacology; D005260:Female; D008297:Male; D010012:Osteogenesis / Q000187:drug effects*; D011458:Prostaglandins E / Q000494:pharmacology*; D013552:Swine
chemical_list: D004041:Dietary Fats; D011458:Prostaglandins E; D016718:Arachidonic Acid