Albumin and fibrinogen adsorption on cibacron blue F3G-A immobilised onto PU-PHEMA (polyurethane-poly(hydroxyethylmethacrylate)) surfaces.
In the present work, it is intended to study the effect of Cibacron blue F3G-A (CB) immobilised onto PU-PHEMA (polyurethane-poly(hydroxyethylmethacrylate)) surfaces on protein adsorption and bacterial adhesion. CB immobilisation was carried out by covalent binding between its triazine ring and the hydroxyl groups of the polymer. Characterisation of the films was carried out by attenuated total reflection Fourier transform infrared spectroscopy (ATR-FT-IR), contact angle measurements. X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). CB efficiency was evaluated using radiolabelled albumin and fibrinogen from pure solutions, mixtures of both and plasma. Bacterial adhesion tests before and after albumin pre-coating were also performed. The presence of CB increases albumin and fibrinogen adsorption to PU-PHEMA surfaces. The incorporation of CB onto the PU-PHEMA surface also increases bacterial adhesion. Although albumin pre-coating decreases bacterial adhesion onto PU (67% decrease) and PU-PHEMA-CB (80%), bacterial adhesion is always lower on PU and PU-PHEMA surfaces than on PU-PHEMA-CB. These results demonstrate that, in contrast to what has been described for CB bound to dextran, CB immobilisation on PU-PHEMA surfaces presents low selectivity to albumin and increased bacterial adhesion relatively to PU and PU-PHEMA surfaces.
Martins
M C L
MC
INEB-Instituto de Engenharia Biomédica, Laboratório de Biomateriais, Rua do Campo Alegre 823, 4150-180 Porto, Portugal. cmartins@ibmc.up.pt
Wang
D
D
Ji
J
J
Feng
L
L
Barbosa
M A
MA
D000327:Adsorption; D000418:Albumins / Q000737:chemistry / Q000378:metabolism*; D001422:Bacterial Adhesion; D001672:Biocompatible Materials / Q000737:chemistry; D005340:Fibrinogen / Q000737:chemistry / Q000378:metabolism*; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D013212:Staphylococcus epidermidis / Q000502:physiology / Q000648:ultrastructure; D013499:Surface Properties; D014227:Triazines / Q000737:chemistry / Q000378:metabolism*
D000418:Albumins; D001672:Biocompatible Materials; D008567:Membranes, Artificial; D014227:Triazines; D011102:Polyhydroxyethyl Methacrylate; C012842:Cibacron Blue F 3GA; D005340:Fibrinogen