Differences in immunogenicity and vaccine potential of peptides from Schistosoma mansoni glyceraldehyde 3-phosphate dehydrogenase.
Six peptides, A, B1, B, C, D and E derived from the primary sequence of Schistosoma mansoni glyceraldehyde 3-phosphate dehydrogenase (SG3PDH) were selected based on lowest homology to human G3PDH and used for immunization of BALB/c mice. Peptides B1 and D induced immunoglobulin (Ig) G1, IgG2a and IgG2b antibodies that reacted with native and denatured SG3PDH, and were associated with significant (P<0.05) increase in fecundity and burden of challenge worms, respectively. Peptides A, B, C and E elicited a modest cellular immune response, IgG2a and/or IgG2b antibodies, and no effect on challenge worm burden. In contrast, tetrameric multiple antigen peptide (MAP) constructs A, B, C or E elicited strong cellular immune responses and production of IgG1 and/or IgG2a and IgG2b antibodies against the homologous MAP and peptide and SG3PDH. The immune responses were associated with significant (P<0.05) decrease in challenge worm burden for MAP B, and significant (P<0.05) reduction in egg count per worm couple for MAP C or E. The data together indicated the nature and effect of immune responses vary for each SG3PDH-derived peptide.
Tallima
Hatem
H
Zoology Department, Faculty of Science, Cairo University, Cairo, 12613, Egypt.
Montash
Mona
M
Veprek
Pavel
P
Velek
Jiri
J
Jezek
Jan
J
El Ridi
Rashika
R
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis / Q000276:immunology; D000917:Antibody Formation / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000737:chemistry / Q000276:immunology*; D007111:Immunity, Cellular / Q000276:immunology; D007114:Immunization; D007371:Interferon-gamma / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D010270:Parasite Egg Count; D010455:Peptides / Q000737:chemistry / Q000276:immunology*; D012550:Schistosoma mansoni / Q000201:enzymology* / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000469:parasitology / Q000517:prevention & control
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D010455:Peptides; D007371:Interferon-gamma; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases