DNA vaccination protects against botulinum neurotoxin type F.
A DNA vaccine was constructed which expressed the binding domain of Clostridium botulinum neurotoxin serotype F fused to a signal peptide. Three intra-muscular doses fully protected Balb/c mice against 10(4) MLD of serotype F toxin. Priming of the immune response by DNA vaccination followed by a single booster with type F binding domain protein resulted in high levels of antibody against the binding domain. This study demonstrates the utility of DNA vaccination for protection against botulinum neurotoxin type F and indicates that a prime-boost regimen could be an efficient method of generating antibody for passive immune therapy in cases of botulism involving serotype F toxin.
Bennett
Alice M
AM
Defence Science and Technology Laboratory, CBD Porton Down, Salisbury, SP4 0JQ, Wiltshire, UK.
Perkins
Stuart D
SD
Holley
Jane L
JL
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D001905:Botulinum Toxins / Q000276:immunology* / Q000633:toxicity*; D019556:COS Cells; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology; D007136:Immunoglobulins / Q000096:biosynthesis / Q000276:immunology; D007273:Injections, Intramuscular; D051379:Mice; D008807:Mice, Inbred BALB C; D018613:Microscopy, Confocal; D010957:Plasmids / Q000235:genetics; D016019:Survival Analysis; D014611:Vaccination; D019444:Vaccines, DNA / Q000096:biosynthesis / Q000276:immunology*
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D007136:Immunoglobulins; D019444:Vaccines, DNA; D001905:Botulinum Toxins; C096321:botulinum toxin type F