Probing the specificity of human myeloma proteins with a random peptide phage library.
Human myeloma proteins (HMPs) from 10 patients with multiple myeloma (MM) were used to affinity-select peptides from a random phage-display peptide library. Binding peptides were identified for the 10 analysed antibodies (eight, immunoglobulin G (IgG), and two, immunoglobulin A (IgA)). The specificity of the binding was confirmed by competitive experiments using phages and chemically synthesized peptides. Interestingly, some phage-displayed peptides were immuno-selected with HMPs isolated from different patients. Sequence alignments and homology searches revealed a significant homology with human proteins (e.g. neural cell adhesion proteins) and pathogen-derived proteins (e.g. herpes simplex virus capsid proteins). The selected peptides could be useful as targeting agents for myeloma cells expressing surface immunoglobulins.
Dybwad
A
A
Department of Immunology, Molecular Medicine Group, The Norwegian Radium Hospital, Montebello, Norway.
Lambin
P
P
Sioud
M
M
Zouali
M
M
D000595:Amino Acid Sequence; D000912:Antibodies, Neoplasm / Q000378:metabolism; D000918:Antibody Specificity; D001667:Binding, Competitive; D006801:Humans; D007070:Immunoglobulin A / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009101:Multiple Myeloma / Q000235:genetics* / Q000276:immunology*; D009194:Myeloma Proteins / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D019151:Peptide Library; D011485:Protein Binding; D017386:Sequence Homology, Amino Acid
D000912:Antibodies, Neoplasm; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D009194:Myeloma Proteins; D019151:Peptide Library