Amniocentesis with increased cell yield, obtained by filtration and reinjection of the amniotic fluid.
In order to increase the cell numbers obtained by amniocentesis, a new amniocentesis technique has been developed and we report preliminary results. The amniotic fluid is aspirated in the usual manner and reinjected immediately into the amniotic cavity after passage through a filter which retains the cells from the fluid. By this procedure, the cells from a relatively large volume of amniotic fluid are concentrated on the filter while only a small volume of amniotic fluid is finally removed. The method has been tested on 30 pregnant women referred for prenatal diagnosis. The mean culture time was 8.3 days and no immediate side-effects were observed.
Sundberg
K
K
Chromosome Laboratory, Section of Clinical Genetics, Department of Gynecology and Obstetrics, Rigshospitalet, University of Copenhagen, Denmark.
Smidt-Jensen
S
S
Philip
J
J