title: Reducing colonization of Salmonella Enteritidis in chicken by targeting outer membrane proteins.
abstract: OBJECTIVE
  To evaluate the ability of Salmonella enterica ser. Enteritidis outer membrane proteins (OMPs) of 75.6 and 82.3 kDa to inhibit or reduce in vivo colonization of S. Enteritidis on intestinal mucosa in chickens.
  
  
  RESULTS
  Nine-week-old specific-pathogen-free chickens were subcutaneously immunized with 75.6 or 82.3 kDa protein, and challenged with a virulent strain of S. Enteritidis. Chickens were killed, and portions of small intestine and caecum were removed at necropsy. The population of S. Enteritidis attached to chicken intestinal mucosa was determined. The population of S. Enteritidis recovered from the small intestine and caecum of chickens immunized with 75.6 or 82.3 kDa protein was significantly (P < 0.05) lower than that recovered from the control birds.
  
  
  CONCLUSIONS
  Salmonella Enteritidis OMPs 75.6 kDa and 82.3 kDa were effective in reducing colonization of S. Enteritidis on intestinal mucosa in chickens.
  
  
  CONCLUSIONS
  Salmonella Enteritidis OMPs 75.6 or 82.3 kDa could be used as potential vaccines to reduce S. Enteritidis colonization in chickens.
authors.affiliation: Department of Pathobiology, University of Connecticut, Storrs, CT 06269, USA. mazhar.khan@uconn.edu
authors.forename: M I
authors.initials: MI
authors.lastname: Khan
authors.forename: A A
authors.initials: AA
authors.lastname: Fadl
authors.forename: K S
authors.initials: KS
authors.lastname: Venkitanarayanan
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000008:administration & dosage*; D015153:Blotting, Western / Q000379:methods; D002432:Cecum / Q000382:microbiology; D002645:Chickens / Q000382:microbiology*; D007413:Intestinal Mucosa / Q000382:microbiology; D007421:Intestine, Small / Q000382:microbiology; D012477:Salmonella enteritidis / Q000254:growth & development*; D014611:Vaccination / Q000379:methods*
chemical_list: D001425:Bacterial Outer Membrane Proteins