Acetylcholine and ATP hyperpolarize endothelium via activation of different types of Ca(2+)-activated K+ channels.
Experiments on rat thoracic aorta showed that ATP and acetylcholine hyperpolarize endothelial cell via selective activation of low (SK) and intermediate (IR) conductance Ca(2+)-activated K(+)channels, respectively. It was hypothesized that ATP- and acetylcholine-activated Ca(2+)-signals are spatially separated and generated in plasma membrane regions with predominant localization of SK- and IR-channels, respectively.
Marchenko
S M
SM
A. A. Bogomolets Institute of Physiology, National Academy of Science of Ukraine, Kiev. smm@serv.biph.kiev.ua
D000109:Acetylcholine / Q000494:pharmacology*; D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D001030:Apamin / Q000494:pharmacology; D020013:Calcium Signaling / Q000187:drug effects; D018999:Charybdotoxin / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism*; D066298:In Vitro Techniques; D051660:Intermediate-Conductance Calcium-Activated Potassium Channels; D008564:Membrane Potentials / Q000187:drug effects; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000187:drug effects* / Q000378:metabolism*; D024681:Potassium Channels, Calcium-Activated; D051381:Rats; D051640:Small-Conductance Calcium-Activated Potassium Channels
D051660:Intermediate-Conductance Calcium-Activated Potassium Channels; D015221:Potassium Channels; D024681:Potassium Channels, Calcium-Activated; D051640:Small-Conductance Calcium-Activated Potassium Channels; D018999:Charybdotoxin; D001030:Apamin; D000255:Adenosine Triphosphate; D000109:Acetylcholine