Morphology and morphogenesis of severe acute respiratory syndrome (SARS)-associated virus.
After infecting the Vero E6 cells by nasal/throat swabs collected from SARS patients, we studied the SARS-associated virus by electron microscopy and molecular biological technique. The results show that the diameter of newly isolated virus is about 50 nm without envelope or 100 nm with envelope. The virus was proved to be a new coronavirus by RT-PCR and it responded positively to convalescent-phase serum specimen from SARS patients, which is the evidence that this new virus is etiologically linked to the outbreak of SARS. The morphogenesis and distribution of the virus are also discussed in this article.
Zhang
Qin-Fen
QF
State Key Lab for Biocontrol, Zhongshan University, Guangzhou 510275, China.
Cui
Jin-Ming
JM
Huang
Xiao-Jun
XJ
Lin
Wei
W
Tan
Dong-Yan
DY
Xu
Jie-Wei
JW
Yang
Yi-Feng
YF
Zhang
Jing-Qiang
JQ
Zhang
Xin
X
Li
Hui
H
Zheng
Huan-Ying
HY
Chen
Qiu-Xia
QX
Yan
Xin-Ge
XG
Zheng
Kui
K
Wan
Zhuo-Yue
ZY
Huang
Jie-Cheng
JC
D000818:Animals; D002467:Cell Nucleus / Q000821:virology; D002522:Chlorocebus aethiops; D006801:Humans; D007830:Larynx / Q000821:virology; D008854:Microscopy, Electron; D009305:Nasopharynx / Q000821:virology; D009685:Nuclear Envelope / Q000821:virology; D045473:Severe acute respiratory syndrome-related coronavirus / Q000276:immunology / Q000302:isolation & purification / Q000648:ultrastructure*; D045169:Severe Acute Respiratory Syndrome / Q000276:immunology / Q000821:virology*; D014709:Vero Cells / Q000821:virology; D014771:Virion / Q000648:ultrastructure