Immature dendritic cells possess a sugar-sensitive receptor for human mannan-binding lectin.
Mannan-binding lectin (MBL) is a collectin synthesized by the liver and secreted into the bloodstream. It has a receptor for microbial structures in its C-type lectin domain and a separate receptor(s) located within its collagen-like region for autologous phagocytic cells. Here we demonstrate that human peripheral blood adherent cells (monocytes) and monocyte-derived dendritic cells are a source of MBL, and that a novel calcium-dependent and sugar-specific MBL receptor is up-regulated in immature (CD1a-positive) dendritic cells. These findings suggest a previously unsuspected autologous function for MBL, perhaps a regulatory role within the immune system.
Downing
Ian
I
Cell Therapy Group, S.N.B.T.S. National Science Laboratory, Edinburgh, UK.
Koch
Claus
C
Kilpatrick
David C
DC
D000117:Acetylglucosamine / Q000494:pharmacology; D002448:Cell Adhesion / Q000276:immunology; D002454:Cell Differentiation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology / Q000378:metabolism*; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D008358:Mannose / Q000494:pharmacology; D037601:Mannose-Binding Lectin / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D011975:Receptors, Mitogen / Q000378:metabolism*; D015854:Up-Regulation
D037601:Mannose-Binding Lectin; D011975:Receptors, Mitogen; D008358:Mannose; D000117:Acetylglucosamine