title: Plasma interleukin 8 concentrations in obese subjects with impaired glucose tolerance.
abstract: BACKGROUND
  Interleukin 8 (IL-8) is a cytokine with atherogenic properties. In vitro studies revealed that it is produced and secreted by human adipocytes. We recently reported that plasma IL-8 is increased in obese subjects with normal glucose tolerance (NGT). The aim of the present study was to measure plasma IL-8 concentrations in subjects with impaired glucose tolerance (IGT).
  
  
  METHODS
  A total of 44 subjects with marked overweight or obesity (BMI > 27.8 kg/m2), 27 with NGT and 17 with IGT, were recruited for the present study. Plasma IL-8 levels were measured in fasting state, after an oral glucose tolerance test (OGTT) and after euglycemic hyperinsulinemic clamp.
  
  
  RESULTS
  The studied groups did not differ in fasting IL-8 concentrations. Both OGTT and clamp resulted in a significant increase in plasma IL-8. The change in IL-8 after clamp was similar in both groups. In contrast, after OGTT plasma IL-8 levels (IL-8OGTT) were markedly higher in IGT individuals. In IGT, but not NGT group, IL-8OGTT was positively related to postload glucose and negatively to insulin sensitivity.
  
  
  CONCLUSIONS
  Plasma IL-8 concentrations after glucose load are increased in obese IGT subjects in comparison to normoglycemic weight-matched individuals. Increase in plasma IL-8 might be both insulin-mediated (during clamp) and glucose-mediated (during OGTT).
authors.affiliation: Department of Endocrinology, Diabetology and Internal Medicine, Medical University, Bialystok, Poland. mstraczkowski@poczta.onet.pl
authors.forename: Marek
authors.initials: M
authors.lastname: Straczkowski
authors.forename: Irina
authors.initials: I
authors.lastname: Kowalska
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Nikolajuk
authors.forename: Stella
authors.initials: S
authors.lastname: Dzienis-Straczkowska
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Szelachowska
authors.forename: Ida
authors.initials: I
authors.lastname: Kinalska