Taking EPR "snapshots" of the oxidative stress status in human blood.
Assessment of oxidative stress status (OSS) in human tissues is still troublesome. Using an innovative EPR-radical-probe we successfully measured the instantaneous concentration of ROS directly in peripheral blood of athletes and normally active workers during 60 min controlled exercise. The probe employed was bis(1-hydroxy-2,2,6,6-tetramethyl-4-piperidinyl)decandioate, which quantitatively and instantaneously reacts with oxygen-centered radicals (including superoxide) to yield the parent nitroxide, which is sufficiently persistent to be measured by EPR. Our measurements suggest that while at rest normally active individuals may benefit more from antioxidant supplementation than athletes; conversely, during exercise athletes may benefit more from supplementation. Our method allows reliable, quick, and non-invasive quantitative determination of OSS in human peripheral blood.
Paolini
Moreno
M
Department of Pharmacology, University of Bologna, via Irnerio 48, 1-40126, Bologna, Italy.
Valgimigli
Luca
L
Marchesi
Emanuela
E
Trespidi
Silvia
S
Pedulli
Gian Franco
GF
D000328:Adult; D003497:Cyclic N-Oxides / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D006801:Humans; D008297:Male; D009569:Nitric Oxide / Q000737:chemistry; D018384:Oxidative Stress; D010100:Oxygen / Q000097:blood; D017382:Reactive Oxygen Species / Q000097:blood*; D012420:Running / Q000502:physiology; D013113:Spin Labels; D013177:Sports; D013997:Time Factors
D003497:Cyclic N-Oxides; D017382:Reactive Oxygen Species; D013113:Spin Labels; D009569:Nitric Oxide; D010100:Oxygen; C001803:tempol