Oral immunization of cattle with hemagglutinin protein of rinderpest virus expressed in transgenic peanut induces specific immune responses.
Rinderpest is an acute, highly contagious often fatal disease of large and small ruminants, both domestic and wild. Global eradication of rinderpest needs a robust, safe and cost-effective vaccine. The causative agent, rinderpest virus (RPV) is an important member of the genus Morbillivirus in the Paramyxoviridae family. We have generated transgenic peanut (Arachis hypogea L.) plants expressing hemagglutinin protein of RPV and report here, the induction of immune responses in cattle following oral feeding with transgenic leaves expressing hemagglutinin protein without oral adjuvant. Hemagglutinin-specific antibody was detected in the serum as confirmed by immunohistochemical staining of virus-infected cells, and in vitro neutralization of virus infectivity. Oral delivery also resulted in cell-mediated immune responses.
Khandelwal
Abha
A
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, 560012, India.
Lakshmi Sita
G
G
Shaila
M S
MS
D000276:Adjuvants, Immunologic; D000284:Administration, Oral; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000917:Antibody Formation / Q000276:immunology; D000956:Antigens, Viral / Q000096:biosynthesis / Q000276:immunology; D010367:Arachis / Q000737:chemistry* / Q000235:genetics*; D002417:Cattle; D002455:Cell Division / Q000502:physiology; D002522:Chlorocebus aethiops; D006388:Hemagglutinins / Q000008:administration & dosage / Q000276:immunology*; D007115:Immunization Schedule; D007150:Immunohistochemistry; D008214:Lymphocytes / Q000187:drug effects; D009500:Neutralization Tests; D030821:Plants, Genetically Modified; D012302:Rinderpest virus / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology; D014709:Vero Cells; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D000956:Antigens, Viral; D006388:Hemagglutinins; D014614:Vaccines, Synthetic; D014765:Viral Vaccines