title: Drosophila FACT contributes to Hox gene expression through physical and functional interactions with GAGA factor.
abstract: Chromatin structure plays a critical role in the regulation of transcription. Drosophila GAGA factor directs chromatin remodeling to its binding sites. We show here that Drosophila FACT (facilitates chromatin transcription), a heterodimer of dSPT16 and dSSRP1, is associated with GAGA factor through its dSSRP1 subunit, binds to a nucleosome, and facilitates GAGA factor-directed chromatin remodeling. Moreover, genetic interactions between Trithorax-like encoding GAGA factor and spt16 implicate the GAGA factor-FACT complex in expression of Hox genes Ultrabithorax, Sex combs reduced, and Abdominal-B. Chromatin immunoprecipitation experiments indicated the presence of the GAGA factor-FACT complex in the regulatory regions of Ultrabithorax and Abdominal-B. These data illustrate a crucial role of FACT in the modulation of chromatin structure for the regulation of gene expression.
authors.affiliation: Department of Developmental Genetics, National Institute of Genetics, and Graduate University for Advanced Studies, Mishima, Shizuoka-ken 411-8540, Japan.
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Shimojima
authors.forename: Masahiro
authors.initials: M
authors.lastname: Okada
authors.forename: Takahiro
authors.initials: T
authors.lastname: Nakayama
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ueda
authors.forename: Katsuya
authors.initials: K
authors.lastname: Okawa
authors.forename: Akihiro
authors.initials: A
authors.lastname: Iwamatsu
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Handa
authors.forename: Susumu
authors.initials: S
authors.lastname: Hirose
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D002843:Chromatin / Q000378:metabolism* / Q000648:ultrastructure; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D004330:Drosophila / Q000235:genetics* / Q000378:metabolism; D029721:Drosophila Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D005801:Genes, Homeobox; D017344:Genes, Insect; D006609:High Mobility Group Proteins / Q000737:chemistry / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D009707:Nucleosomes / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D035602:Transcriptional Elongation Factors
chemical_list: C062393:Abd-B proteins, Drosophila; D002843:Chromatin; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D006609:High Mobility Group Proteins; D018398:Homeodomain Proteins; D009707:Nucleosomes; C489543:SSRP protein, Drosophila; C059627:Scr protein, Drosophila; D014157:Transcription Factors; D035602:Transcriptional Elongation Factors; C063460:Trl protein, Drosophila; C066177:Ubx protein, Drosophila