title: Fusion to a carrier protein and a synthetic propeptide enhances E7 HPV-16 production and secretion in Lactococcus lactis.
abstract: An inducible system to improve and stabilize the production of an extremely labile protein (E7 antigen of human papillomavirus type 16) was developed in the food-grade bacterium Lactococcus lactis. A protein carrier, the staphylococcal nuclease Nuc, was fused either to N- or C-termini of E7 protein, and the resulting hybrid proteins were rescued from intracellular proteolysis but poorly secreted by L. lactis. A synthetic propeptide (LEISSTCDA) was then fused and significantly improved the secretion efficiency of the hybrid protein Nuc-E7 by L. lactis.
authors.affiliation: Laboratorio de Inmunología y Virología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, NL México.
authors.forename: Luis G
authors.initials: LG
authors.lastname: Bermúdez-Humarán
authors.forename: Naima G
authors.initials: NG
authors.lastname: Cortes-Perez
authors.forename: Yves
authors.initials: Y
authors.lastname: Le Loir
authors.forename: Alexandra
authors.initials: A
authors.lastname: Gruss
authors.forename: Cristina
authors.initials: C
authors.lastname: Rodriguez-Padilla
authors.forename: Odila
authors.initials: O
authors.lastname: Saucedo-Cardenas
authors.forename: Philippe
authors.initials: P
authors.lastname: Langella
authors.forename: Roberto
authors.initials: R
authors.lastname: Montes de Oca-Luna
mesh_terms: D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D013294:Lactococcus lactis / Q000235:genetics* / Q000378:metabolism*; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis* / Q000235:genetics*; D050725:Papillomavirus E7 Proteins; D015202:Protein Engineering / Q000379:methods*; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism; D011786:Quality Control; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D011498:Protein Precursors; D011993:Recombinant Fusion Proteins; C059731:oncogene protein E7, Human papillomavirus type 16