title: Selective repression of c-fos gene transcription in rat embryo fibroblasts transformed by oncogenes E1A and cHa-ras.
abstract: Transformation of REF cells by oncogenes E1A and cHa-ras leads to activation of AP-1 factor concomitantly with down-regulation of c-fos gene transcription. Here we addressed two issues: (i) how does transcription of Fos/Jun-regulated genes change in the cells lacking Fos-Jun heterodimers; (ii) to which extent HDAC-mediated chromatin reorganization does affect, apart from c-fos, transcription of some other early and late-response genes. To this end, we studied the kinetics of serum-stimulated transcription of c-fos, c-jun, fra-1, egr-1, and cyclinD1 genes, as well as the effects of sodium butyrate, an inhibitor of histone deacetylase activity, on transcription of these genes in normal REF cells and transformants E1A+ras.
authors.affiliation: Institute of Cytology, Russian Academy of Sciences, 194064, St. Petersburg, Russia.
authors.forename: M V
authors.initials: MV
authors.lastname: Abramova
authors.forename: A N
authors.initials: AN
authors.lastname: Kukushkin
authors.forename: S B
authors.initials: SB
authors.lastname: Svetlikova
authors.forename: T V
authors.initials: TV
authors.lastname: Pospelova
authors.forename: V A
authors.initials: VA
authors.lastname: Pospelov
mesh_terms: D017500:Adenovirus E1A Proteins / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D002843:Chromatin / Q000378:metabolism; D019281:Dimerization; D005347:Fibroblasts / Q000378:metabolism; D006655:Histone Deacetylases / Q000378:metabolism; D007700:Kinetics; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012978:Sodium Oxybate / Q000494:pharmacology; D014158:Transcription, Genetic; D018631:ras Proteins / Q000378:metabolism
chemical_list: D017500:Adenovirus E1A Proteins; D002843:Chromatin; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D012978:Sodium Oxybate; D006655:Histone Deacetylases; D018631:ras Proteins