Antibodies in proteomics I: generating antibodies.
The explosion in genome sequencing, and in subsequent DNA array experiments, has provided extensive information on gene sequence, organization and expression. This has resulted in a desire to perform similarly broad experiments on all the proteins encoded by a genome. Panels of specific antibodies, or other binding ligands, will be essential tools in this endeavour. Because traditional immunization will be unlikely to generate antibodies in sufficient quantity, and of the required quality and reproducibility, in vitro selection methods will probably be used. This review--the first of two--examines the strategies available for in vitro antibody selection. The second review discusses the adaptation of these methods to high throughput and the uses to which antibodies, once derived, can be put.
Bradbury
Andrew
A
B Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA. amb@lanl.gov
Velappan
Nileena
N
Verzillo
Vittorio
V
Ovecka
Milan
M
Chasteen
Leslie
L
Sblattero
Daniele
D
Marzari
Roberto
R
Lou
Jianlong
J
Siegel
Robert
R
Pavlik
Peter
P
D000818:Animals; D000906:Antibodies / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D000917:Antibody Formation / Q000502:physiology*; D006801:Humans; D019151:Peptide Library; D015202:Protein Engineering / Q000379:methods*; D040901:Proteomics / Q000379:methods*; D012641:Selection, Genetic
D000906:Antibodies; D019151:Peptide Library