Analysis of DNA evidence recovered from epithelial cells in penile swabs.
In the rape case presented here, no semen, hair, or other biological evidence were left by the perpetuator at the crime scene or on the victim. The alleged assailant was arrested soon after the crime. A classical stain recovery technique using cotton swab moistened with sterile water was taken for recovering potential female epithelial cells and leukocytes deposited on the alleged assailant's penis during sexual assault. The organic method used for DNA extraction was quantified according to the slot-blot procedure and amplified at 9 and 15 polymorphic loci. Penile swab revealed a DNA profile of mixed origin. In addition to the suspect's DNA profile, DNA contribution from the victim was identified as a minor component in the mixture. Frequency of the profile resulted in a value of 5 x 10(-14) for the multiplex systems AmpFlSTR Plus and 2.5 x 10(-18) for the multiplex system PowerPlex 16, taking into account only non-overlapping alleles between the suspect and the victim from the minor component in the DNA mixture. Moreover, three additional alleles were observed at D21S11 locus by use of PowerPlex and STR SGM plus primers, which could not belong to the suspect. The victim's DNA profile showed the same three-banded genotype at this locus. The same pattern was detected when the victim's saliva or blood were used as reference samples. Our laboratory finding was consistent with the police report that the victim was a person with Down syndrome, a human genetic disease mainly resulting from trisomy (triplication) of the 21 chromosome.
Drobnic
Katja
K
Forensic Lab and Research Center, Ministry of Interior, Vodovodna 95, 1000 Ljubljana, Slovenia. katja.drobnic@mnz.si
D000293:Adolescent; D000328:Adult; D016172:DNA Fingerprinting / Q000379:methods; D004847:Epithelial Cells; D005260:Female; D005554:Forensic Medicine; D006801:Humans; D008297:Male; D010413:Penis / Q000166:cytology*; D011902:Rape