title: Usefulness of 8 kDa protein of Fasciola hepatica in diagnosis of fascioliasis.
abstract: This study was designed to detect and evaluate an antigenicity of low molecular weight proteins of Fasciola hepatica in fascioliasis. Low molecular weight protein of F. hepatica was purified by ammonium sulfate precipitation and Sephacryl S-100 HR gel filtration. The protein obtained was estimated to be 8 kDa on 7.5-15% gradient sodium dodecyl sulfate gel electrophoresis. Immunoblotting studies showed that the 8 kDa protein reacted with human fascioliasis sera, but not other trematodiasis sera. This result suggests that the 8 kDa protein of F. hepatica is one of diagnostic antigens in human fascioliasis without cross-reaction with other human trematodiasis.
authors.affiliation: Department of General Surgery, Cheju National University Hospital, Jeju 690-716, Korea.
authors.forename: Kwangsig
authors.initials: K
authors.lastname: Kim
authors.forename: Hyun Jong
authors.initials: HJ
authors.lastname: Yang
authors.forename: Young-Bae
authors.initials: YB
authors.lastname: Chung
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D005210:Fasciola hepatica / Q000276:immunology / Q000302:isolation & purification*; D005211:Fascioliasis / Q000097:blood / Q000469:parasitology*; D015801:Helminth Proteins / Q000302:isolation & purification*; D006801:Humans; D015151:Immunoblotting
chemical_list: D000947:Antigens, Helminth; D015801:Helminth Proteins