title: The stability of dihydropyrimidine dehydrogenase activity in colorectal cancer tissue after resection.
abstract: Dihydropyrimidine dehydrogenase (DPD) is the first and rate-limiting enzyme in the catabolism of 5-fluorouracil (5-FU). DPD activity in tumor tissue may consequently serve as a predictive factor for sensitivity to 5-FU. The purpose of this study was to elucidate the stability of DPD activity over time in resected colorectal cancer tissue. DPD activity was measured in samples frozen immediately after the tumor was resected and in samples that had been allowed to stand at room temperature for 15, 30, 60, 120, and 180 min before being frozen. Surgical specimens obtained from 15 cases with advanced colorectal cancer were analyzed. The effects of differences among individuals and differences in time between collection and freezing were assessed by two-way analysis of variance (ANOVA). Significant differences in DPD activity were found among tumors (p=0.001), but there were no significant differences in the same tumor depending on the time between collection and freezing (p=0.496). Based on these results, it appears possible to make comparative assessments of DPD activity in colorectal cancer tissue without strictly stipulating the time between resection and freezing.
authors.affiliation: Department of Surgery, Tokai University School of Medicine, Bohseidai, Isehara-shi, Kanagawa 259-1193, Japan. sadahiro@is.icc.u-tokai.ac.jp
authors.forename: Sotaro
authors.initials: S
authors.lastname: Sadahiro
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Suzuki
authors.forename: Kenji
authors.initials: K
authors.lastname: Ishikawa
authors.forename: Tomoki
authors.initials: T
authors.lastname: Nakamura
authors.forename: Akemi
authors.initials: A
authors.lastname: Kamijo
authors.forename: Takeshi
authors.initials: T
authors.lastname: Saguchi
authors.forename: Seiei
authors.initials: S
authors.lastname: Yasuda
authors.forename: Tomoo
authors.initials: T
authors.lastname: Tajima
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Makuuchi
authors.forename: Chieko
authors.initials: C
authors.lastname: Murayama
mesh_terms: D000328:Adult; D000368:Aged; D001706:Biopsy; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000601:surgery; D042943:Dihydrouracil Dehydrogenase (NADP) / Q000378:metabolism*; D004795:Enzyme Stability; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D013997:Time Factors
chemical_list: D042943:Dihydrouracil Dehydrogenase (NADP)