Neurodegeneration of substantia nigra accompanied with macrophage/microglia infiltration after intrastriatal hemorrhage.
Intrastriatal hemorrhage in rats causes neurodegenaration of the substantia nigra (SN) followed by the appearance of ED1(+) cells (macrophage/microglia). ED1(+) cells were observed for at least 8 weeks after hemorrhage. Phosphorylation of p38 mitogen-activated protein kinase (MAPK) was shown in ED1(+) cells with the expression of both brain-derived neurotrophic factor (BDNF) mRNA and BDNF, suggesting that activated-p38 MAPK(+)/ED1(+) cells would produce BDNF and may exhibit trophic effect on the degenerating neurons in the SN. However, in ELISA, BDNF protein decreased significantly in ipsilateral SN at 7 days after hemorrhage, which may be due to a dramatic decrease of BDNF immunoreactive neurons in pars compacta. Data suggest that activation of p38 MAPK in ED1(+) cells infiltrating in ipsilateral SN after hemorrhage may produce BDNF, but that the amount of BDNF produced from ED1(+) cells is insufficient for the rescue of degenerating neurons.
Imamura
Nobuki
N
Department of Neurosurgery and Restorative Neuroscience, Nagoya City University Graduate School of Medical Sciences, Kawasumi 1, Mizoho-cho, Mizuho-ku, 467-8601, Nagoya, Japan.
Hida
Hideki
H
Aihara
Noritaka
N
Ishida
Kazuto
K
Kanda
Yoshie
Y
Nishino
Hitoo
H
Yamada
Kazuo
K
D000818:Animals; D001058:Apomorphine / Q000494:pharmacology; D001522:Behavior, Animal; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis / Q000235:genetics; D000697:Central Nervous System Stimulants / Q000494:pharmacology; D002543:Cerebral Hemorrhage / Q000139:chemically induced / Q000150:complications* / Q000378:metabolism / Q000473:pathology; D017364:Collagenases / Q000633:toxicity; D004195:Disease Models, Animal; D018491:Dopamine Agonists / Q000494:pharmacology; D053331:Ectodysplasins; D004797:Enzyme-Linked Immunosorbent Assay; D007839:Functional Laterality; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008264:Macrophages / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D008694:Methamphetamine / Q000494:pharmacology; D017628:Microglia / Q000378:metabolism* / Q000473:pathology; D020928:Mitogen-Activated Protein Kinases / Q000096:biosynthesis; D009410:Nerve Degeneration / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D012399:Rotation; D013378:Substantia Nigra / Q000378:metabolism / Q000473:pathology*; D013997:Time Factors; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases
D019208:Brain-Derived Neurotrophic Factor; D000697:Central Nervous System Stimulants; D018491:Dopamine Agonists; D053331:Ectodysplasins; D008565:Membrane Proteins; D012333:RNA, Messenger; D008694:Methamphetamine; D014446:Tyrosine 3-Monooxygenase; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases; D017364:Collagenases; D001058:Apomorphine