Functional genomics of sleep.
Functional genomics is a systematic and high-throughput effort to analyze the functions of genes and gene products. Functional genomics is divided into gene- and phenotype-driven approaches. Gene-driven approaches to the functional genomics of sleep have demonstrated that transcripts of many genes change as a function of behavioral state. A phenotype-driven approach includes identification and characterization of gene function through the analyses of natural polygenic traits, creation of transgenic animals or high-throughput mutagenesis. Identification of a gene for narcolepsy through QTL analyses and concomitantly using a transgenic approach is one example of the phenotype-driven approach to the functional genomics of sleep. Though the majority of functional genomics is currently performed in mice, the rat is emerging as an important model for genomic research. Since rest in Drosophila shares many features with mammalian sleep, this allows a comparative functional genomics approach to the study of rest and sleep. The concepts outlined here for the functional genomics of sleep are applicable to respiration research.
Mackiewicz
Miroslaw
M
Department of Medicine, Division of Sleep Medicine, Center for Sleep and Respiratory Neurobiology, Hospital of the University of Pennsylvania, 991 Maloney Building, Philadelphia, PA 19104-4283, USA.
Pack
Allan I
AI
D000818:Animals; D023281:Genomics / Q000379:methods*; D006801:Humans; D051379:Mice; D008954:Models, Biological; D010641:Phenotype; D051381:Rats; D012119:Respiration / Q000235:genetics; D012890:Sleep / Q000235:genetics* / Q000502:physiology; D014851:Wakefulness / Q000235:genetics / Q000502:physiology