Immunohistochemical characterization of macrophage and dendritic cell subpopulations of the spleen, thymus, tongue and heart in cyclophosphamide-induced immunosuppressed rat.
This study was undertaken to investigate the immunohistochemical characterization of different subpopulations of macrophages and dendritic cells (DCs) of the spleen, thymus, tongue and heart in cyclophosphamide (CY)-induced immunosuppressed rat. After CY treatment, remarkably, ED1+, ED2+ and ED3+ macrophage subpopulations, in general exhibited signs of cellular activation such as an increase in number and size of cell, and an upregulation of the ED1, ED2 and ED3 reactive surface molecule expression in all the organs studied, except for some macrophage subpopulations including ED1+ macrophages in the non-lymphoid tissues. Subpopulations of DCs showed a differential sensitivity to CY. Lymphoid DCs were more sensitive to CY than non-lymphoid interstitial DCs. CY induced a conspicuous upregulation of intercellular adhesion molecule-1 (ICAM-1) expression in the vascular endothelial cells, splenic marginal zone and thymic cortex. In this study, we demonstrated the in vivo effects of CY treatment on subpopulations of macrophages and DCs as well as on ICAM-1 expression in the rat spleen, thymus, tongue and heart. Moreover, our results shed more light on the activation effects of CY on certain subpopulations of macrophages, on the differential sensitivity of DCs to CY between the immature and mature ones, on the functional role of different subpopulations of macrophages, and on the significance of upregulated ICAM-1 expression in the splenic marginal zone and thymic cortex after CY treatment.
Yoon
S
S
Department of Anatomy, College of Medicine, Pusan National University, Pusan, South Korea. sikyoon@pusan.ac.kr
Yoo
H J
HJ
Shim
N R
NR
Baek
S Y
SY
Kim
B S
BS
Kim
J B
JB
Jun
E J
EJ
Son
Y K
YK
Lee
S Y
SY
Yoo
Y H
YH
D000818:Animals; D003520:Cyclophosphamide / Q000494:pharmacology*; D003713:Dendritic Cells / Q000145:classification / Q000187:drug effects* / Q000276:immunology; D007150:Immunohistochemistry / Q000662:veterinary; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis; D008264:Macrophages / Q000145:classification / Q000187:drug effects* / Q000276:immunology; D008297:Male; D009206:Myocardium / Q000166:cytology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects; D014059:Tongue / Q000166:cytology / Q000187:drug effects
D007166:Immunosuppressive Agents; D018799:Intercellular Adhesion Molecule-1; D003520:Cyclophosphamide