Membrane cartridges for endotoxin removal from interferon preparations.
The suitability of membrane cartridges for the removal of endotoxin from both distilled water and interferon preparations was examined. The endotoxin concentrations were reduced to 4.0 and 7.3 EU/ml, respectively, when about 4000 ml of distilled water with 20 and 28 EU/ml were passed through the deoxycholate and chitosan immobilized membrane cartridges. When 200 ml of interferon preparation with endotoxin concentration more than 80 EU/ml and pH 3.9 were applied to a deoxycholate immobilized membrane cartridge at a flow-rate of 9 ml/min, the endotoxin concentration was reduced to less than 10 EU/ml. However, if an interferon preparation of 450 ml, with more than 80 EU/ml of endotoxin and pH 3.9 was applied to the chitosan immobilized membrane cartridge at a flow-rate of 18 ml/min, the endotoxin concentration was reduced to less than 10 EU/ml.
Li
Jinghua
J
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 161 Zhongshan Road, Dalian 116012, PR China.
Shao
Yingguang
Y
Chen
Zhaoan
Z
Cong
Runzi
R
Wang
Junde
J
Liu
Xueliang
X
D000327:Adsorption; D004731:Endotoxins / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007372:Interferons / Q000737:chemistry*; D008567:Membranes, Artificial
D004731:Endotoxins; D008567:Membranes, Artificial; D007372:Interferons