title: Metabolism of SFZ-47 in chicken embryo by liquid chromatography-electrospray ion trap mass spectrometry.
abstract: OBJECTIVE
  To develop an alternative method for investigation of drug metabolism by fertilized chicken eggs using 3H-1,2-dihydro-2-(4-methyl-phenylamino) methyl-1-pyrrolizinone (SFZ-47) as a probe drug.
  
  
  METHODS
  SFZ-47 (15 mg) was injected into the albumen of eggs from standardized breed chickens previously incubated for 10 d. After 72 h of further incubation, the allantoic liquid was subjected to solid phase extraction on XAD-2 columns and analyzed by liquid chromatography-electrospray ion trap mass spectrometry method.
  
  
  RESULTS
  Three major metabolites were identified, namely 4-(3H-1,2-dihydro-1-pyrrolizinone-2-methyl-amino) benzyl alcohol (SFZ-47-OH), 4-(3H-1,2-dihydro-1-pyrrolizinone-2-methyl-amino)-benzoic acid (SFZ-47-COOH), and its glucuronide conjugates. The metabolic profile was little different from that previously found in rabbits and dogs.
  
  
  CONCLUSIONS
  The result demonstrates the usefulness of the fertilized chicken egg as a convenient source of both phase I and phase II metabolites for further metabolism studies of SFZ-47.
authors.affiliation: Research Center for Drug Metabolism, Jilin University, Changchun 130023, China.
authors.forename: Qing-Guang
authors.initials: QG
authors.lastname: Dong
authors.forename: Jing-Kai
authors.initials: JK
authors.lastname: Gu
authors.forename: Da-Fang
authors.initials: DF
authors.lastname: Zhong
authors.forename: J Paul
authors.initials: JP
authors.lastname: Fawcett
authors.forename: Hui
authors.initials: H
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002642:Chick Embryo; D002853:Chromatography, Liquid; D011758:Pyrroles / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: C460139:3H-1,2-dihydro-2-(4-methylphenylamino)methyl-1-pyrrolizinone; D011758:Pyrroles