title: Phospholipase Cdelta1 is required for skin stem cell lineage commitment.
abstract: Phosphoinositide-specific phospholipase C (PLC) is a key enzyme in phosphoinositide turnover and is involved in a variety of physiological functions. Here we report that PLCdelta(1)-deficient mice undergo progressive hair loss in the first postnatal hair cycle. Epidermal hyperplasia was observed, and many hairs in the skin of PLCdelta(1)-deficient mice failed to penetrate the epidermis and became zigzagged owing to occlusion of the hair canal. Two major downstream signals of PLC, calcium elevation and protein kinase C activation, were impaired in the keratinocytes and skin of PLCdelta(1)-deficient mice. In addition, many cysts that had remarkable similarities to interfollicular epidermis, as well as hyperplasia of sebaceous glands, were observed. Furthermore, PLCdelta(1)-deficient mice developed spontaneous skin tumors that had characteristics of both interfollicular epidermis and sebaceous glands. From these results, we conclude that PLCdelta(1) is required for skin stem cell lineage commitment.
authors.affiliation: Department of Biochemistry, The Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Fukami
authors.forename: Haiyan
authors.initials: H
authors.lastname: Yu
authors.forename: Kei
authors.initials: K
authors.lastname: Takenaka
authors.forename: Yuki
authors.initials: Y
authors.lastname: Kataoka
authors.forename: Yuji
authors.initials: Y
authors.lastname: Shirakata
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Nishikawa
authors.forename: Koji
authors.initials: K
authors.lastname: Hashimoto
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Yoshida
authors.forename: Tadaomi
authors.initials: T
authors.lastname: Takenawa
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D019070:Cell Lineage; D000078404:Epidermal Cells; D004814:Epidermal Cyst / Q000378:metabolism / Q000473:pathology; D004817:Epidermis / Q000473:pathology; D018390:Gene Targeting; D006201:Hair Diseases / Q000235:genetics / Q000378:metabolism; D018859:Hair Follicle / Q000166:cytology / Q000473:pathology / Q000502:physiology; D006801:Humans; D006965:Hyperplasia; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D015603:Keratinocytes / Q000166:cytology / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D054803:Phospholipase C delta; D011493:Protein Kinase C / Q000378:metabolism; D012627:Sebaceous Glands / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D015398:Signal Transduction / Q000502:physiology; D012867:Skin / Q000166:cytology* / Q000254:growth & development / Q000473:pathology; D013234:Stem Cells / Q000502:physiology*; D010738:Type C Phospholipases / Q000235:genetics / Q000378:metabolism*
chemical_list: D007527:Isoenzymes; D011493:Protein Kinase C; D010738:Type C Phospholipases; D054803:Phospholipase C delta; D002118:Calcium