title: [Expression of different RASSF1 transcripts and its clinical significance in lung carcinoma].
abstract: OBJECTIVE
  To evaluate the expression of three different RASSF1 transcripts and its clinical significance in lung carcinomas.
  
  
  METHODS
  The mRNA expression of RASSF1A, RASSF1B and RASSF1C was detected by RT-PCR in 51 human lung cancer tissues and 51 matched normal tissues.
  
  
  RESULTS
  1. The mRNA expression of three RASSF1 transcripts was detectable in all non-cancer tissues. However, high rate of expression loss of RASSF1A and RASSF1B existed in lung cancer tissues, which was 53.2% (2851) and 37.3% (19/51), respectively. RASSF1C was expressed in all of the tumor tissues. 2. Loss or abnormal down-regulation of RASSF1A was positively related with lymph node metastasis and TNM stage (P < 0.05) and 3. RASSF1B and RASSF1C mRNA expression was not correlated with TNM stage, histological type, differentiation grade or smoking index.
  
  
  CONCLUSIONS
  There is a significant expression difference among the three RASSF1 transcripts in lung carcinoma. RASSF1A, closely associated with lymph metastasis and TNM stage of lung carcinoma, should be a new tumor suppressor gene.
authors.affiliation: Laboratory of Thoracic Surgical Oncology, Cancer Institute (Hospital), Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100021, China.
authors.forename: Kang
authors.initials: K
authors.lastname: Shao
authors.forename: Jie
authors.initials: J
authors.lastname: He
authors.forename: Bang-chang
authors.initials: BC
authors.lastname: Cheng
authors.forename: Zhi-xiong
authors.initials: ZX
authors.lastname: Xiao
authors.forename: De-chao
authors.initials: DC
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Cui-yan
authors.initials: CY
authors.lastname: Zhang
authors.forename: Fang
authors.initials: F
authors.lastname: Zhou
authors.forename: Mei-hua
authors.initials: MH
authors.lastname: Xiong
authors.forename: Huai-jing
authors.initials: HJ
authors.lastname: Tang
authors.forename: Su-sheng
authors.initials: SS
authors.lastname: Shi
authors.forename: Ba-dong
authors.initials: BD
authors.lastname: Mu
mesh_terms: D002872:Chromosome Deletion; D002893:Chromosomes, Human, Pair 3; D016147:Genes, Tumor Suppressor; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008207:Lymphatic Metastasis; D009367:Neoplasm Staging; D012333:RNA, Messenger / Q000032:analysis*; D025521:Tumor Suppressor Proteins / Q000235:genetics*
chemical_list: C411762:RASSF1 protein, human; D012333:RNA, Messenger; D025521:Tumor Suppressor Proteins