Quantitative analysis of Fas and Fas ligand mRNAs in a feline T-lymphoid cell line after infection with feline immunodeficiency virus and primary peripheral blood mononuclear cells obtained from cats infected with the virus.
Apoptosis is frequently observed in feline lymphocytes in association with feline immunodeficiency virus (FIV) infection. In this study, to investigate the mechanism of FIV-induced apoptosis, levels of Fas and Fas ligand mRNAs were measured by real-time reverse transcription-PCR. In a feline T-lymphoid cell line the amounts of Fas ligand mRNA increased along with the induction of apoptosis after in vitro infection with FIV. In PBMC collected from 10 cats naturally infected with FIV, Fas ligand mRNA levels were significantly higher than those in PBMC from five uninfected cats. These results indicate that the increased expression of Fas ligand may be involved in the induction of apoptosis of lymphocytes in FIV infection.
Mizuno
T
T
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Goto
Y
Y
Baba
K
K
Momoi
Y
Y
Endo
Y
Y
Nishimura
Y
Y
Masuda
K
K
Ohno
K
K
Tsujimoto
H
H
D000818:Animals; D017209:Apoptosis / Q000235:genetics; D002371:Cat Diseases / Q000235:genetics / Q000473:pathology / Q000821:virology; D002415:Cats; D002460:Cell Line; D053222:Fas Ligand Protein; D016181:Feline Acquired Immunodeficiency Syndrome / Q000235:genetics* / Q000473:pathology / Q000821:virology; D005786:Gene Expression Regulation; D016089:Immunodeficiency Virus, Feline / Q000502:physiology*; D007963:Leukocytes, Mononuclear / Q000378:metabolism / Q000473:pathology* / Q000821:virology*; D016399:Lymphoma, T-Cell / Q000235:genetics / Q000473:pathology / Q000821:virology; D008562:Membrane Glycoproteins / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D019014:fas Receptor / Q000235:genetics*
D053222:Fas Ligand Protein; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D019014:fas Receptor