HPLC analysis of metabolically produced formaldehyde.
Radiolabelled (-)-deprenyl is orally administered to rats, and urinary elimination of radiolabelled formaldehyde is detected. The separation is performed using high-performance liquid chromatography on octadecyl-silica stationary phase. Both the radioactivity and the UV absorbance of the dinitrophenylhydrazine formaldehyde peak are determined. Formaldehyde generation takes place by N-demethylation. Low levels of formaldehyde may have a beneficial role in counterbalancing the oxidative stress of the everyday person's life.
Lengyel
J
J
Semmelweis University, 1089 Budapest, Nagyvárad ter 4, Hungary.
Kalász
H
H
Szarvas
T
T
Peltz
Cs
C
Szarkáné-Bolehovszky
A
A
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005557:Formaldehyde / Q000652:urine*; D008297:Male; D013058:Mass Spectrometry; D051381:Rats; D012015:Reference Standards
D005557:Formaldehyde