Immobilization of histidine-tagged proteins on nickel by electrochemical dip pen nanolithography.
Dip-pen nanolithography (DPN) is becoming a popular technique to "write" molecules on a surface by using the tip of an atomic force microscope (AFM) coated with the desired molecular "ink". In this work, we demonstrate that poly-histidine-tagged peptides and proteins, and free-base porphyrins coated on AFM probes, can be chelated to ionized regions on a metallic nickel surface by applying an electric potential to the AFM tip in the DPN process. DPN has been accomplished in the Tapping Mode of AFM, which creates many possible applications of positioning and subsequently imaging biomolecules, especially on soft surfaces.
Agarwal
Gunjan
G
Materials and Manufacturing Directorate, Air Force Research Laboratory, Wright-Patterson Air Force Base, Ohio 45433, USA.
Naik
Rajesh R
RR
Stone
Morley O
MO
D004563:Electrochemistry / Q000379:methods; D006639:Histidine / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D036103:Nanotechnology / Q000379:methods; D009532:Nickel / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry; D010446:Peptide Fragments / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D011166:Porphyrins / Q000737:chemistry; D016271:Proto-Oncogene Proteins c-myc / Q000737:chemistry; D055672:Static Electricity
D009942:Organometallic Compounds; D010446:Peptide Fragments; D010455:Peptides; D011166:Porphyrins; D016271:Proto-Oncogene Proteins c-myc; C033223:polyhistidine; D006639:Histidine; D009532:Nickel