Bioelectronic DNA detection of human papillomaviruses using eSensor: a model system for detection of multiple pathogens.
BACKGROUND
We used human papillomaviruses (HPV) as a model system to evaluate the utility of a nucleic acid, hybridization-based bioelectronic DNA detection platform (eSensor) in identifying multiple pathogens.


METHODS
Two chips were spotted with capture probes consisting of DNA oligonucleotide sequences specific for HPV types. Electrically conductive signal probes were synthesized to be complementary to a distinct region of the amplified HPV target DNA. A portion of the HPV L1 region that was amplified by using consensus primers served as target DNA. The amplified target was mixed with a cocktail of signal probes and added to a cartridge containing a DNA chip to allow for hybridization with complementary capture probes.


RESULTS
Two bioelectric chips were designed and successfully detected 86% of the HPV types contained in clinical samples.


CONCLUSIONS
This model system demonstrates the potential of the eSensor platform for rapid and integrated detection of multiple pathogens.
Vernon
Suzanne D
SD
Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA. svernon@cdc.gov
Farkas
Daniel H
DH
Unger
Elizabeth R
ER
Chan
Vivian
V
Miller
Donna L
DL
Chen
Yin-Peng
YP
Blackburn
Gary F
GF
Reeves
William C
WC
D015374:Biosensing Techniques / Q000379:methods; D017931:DNA Primers; D015344:DNA Probes, HPV; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D027383:Papillomaviridae / Q000302:isolation & purification*
D017931:DNA Primers; D015344:DNA Probes, HPV