Trypsinogen hL is not a new member of the human trypsinogen family, but a known mouse ortholog.
An allegedly novel human trypsinogen cDNA clone termed trypsinogen hL was recently reported in the Biological & Pharmaceutical Bulletin (2003; 26: 361-364). This "new" trypsinogen sequence was isolated by PCR using human lung cDNAs as template and was presumed to be a new member of the human trypsinogen family. However, trypsinogen hL does not match any sequence in the human genome; whereas it is 100% identical to mouse trypsinogen 7. Thus, trypsinogen hL is not of human origin, and its cloning from a human cDNA library was clearly a result of contamination with mouse genetic material. Publication of this cloning artifact could have been avoided by a simple BLAST search of GenBank or other sequence databanks.
Chen
Jian-Min
JM
INSERM-EMI 0115, Génétique Moléculaire et Génétique Epidémiologique, Université de Bretagne Occidentale, Etablissement Francais du Sang-Bretagne, Centre Hospitalier Universitaire, Brest 29275, France.
Férec
Claude
C
Sahin-Tóth
Miklós
M
D000818:Animals; D003001:Cloning, Molecular; D006801:Humans; D008168:Lung / Q000378:metabolism*; D051379:Mice; D014362:Trypsinogen / Q000096:biosynthesis / Q000145:classification* / Q000235:genetics
C477927:Trypsinogen hL , human; D014362:Trypsinogen