[Neural stem and progenitor cells of human embryos and fetuses as a basis of biomedical new technologies].
Isolation and cultivation of stem and progenitor cells of human embryos and fetuses at the age of 7-12 weeks of gestation have been described. The embryonic cells of human brain formed neurospheres with heterogenous composition. Cell differentiation took place not only in the presence of serum or as a result of attachment of neurosphere to a sublayer, but also in floating neurospheres in the presence of mitogens. In most neurospheres, the nestin-immunopositive cells were located near the surface while the cells stained for beta-tubulin III and glial fibrillar acid protein, as compact groups inside the neurospheres.
Poltavtseva
R A
RA
Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, ul. Vavilova 26, Moscow, 119991 Russia. rim-poltavtseva@mail.ru
Revishchin
A V
AV
Aleksandrova
M A
MA
Korochkin
L I
LI
Viktorov
I V
IV
Sukhikh
G T
GT
D001709:Biotechnology / Q000379:methods / Q000639:trends; D001798:Blood Proteins / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology*; D005260:Female; D006801:Humans; D008934:Mitogens / Q000494:pharmacology; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D011247:Pregnancy; D018874:Spheroids, Cellular; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D014404:Tubulin / Q000276:immunology / Q000378:metabolism
D001798:Blood Proteins; D008934:Mitogens; D014404:Tubulin