[Methoxypolyethylene glycol-modified HLA-A2 antigen on lymphocytes].
OBJECTIVE
To modify the HLA-A2 antigen on the lymphocytes with methoxypolyethylene glycol (mPEG) so as to block the specific binding site for antibody.


METHODS
Different types of mPEG (all with final concentration of 12 mmol/L) were used at different temperatures in PBS with varied pH values for the modification of the HLA-A2 antigen.


RESULTS
The modification of the antigen was not obviously affected when it was carried out at 4 degrees Celsius or room temperature, but higher temperatures of 30 and 37 degrees Celsius significantly hampered the modification. Better antigen modification was observed with high-concentration mPEG in basic PBS, depending also on the type of mPEGs used for this purpose.


CONCLUSIONS
The specific HLA-A2 binding on the lymphocytes is completely blocked by benzotriazole carbonate-mPEG(mPEG-BTC), which is superior to N-hydroxysuccinimidyl ester of mPEG(mPEG-SPA). Maleimide-mPEG(mPEG-MAL) is incapable of blocking the HLA-A2 ligand-binding site with antibody.
Zhang
Yin-ze
YZ
Department of Blood Transfusion, First Military Medical University, Guangzhou 510515, China. zyz200157@sohu.com
Li
Wei
W
Zhou
Hua-you
HY
Shan
Xiao-yan
XY
Lan
Jiong-cai
JC
Zhang
Yang-pei
YP
Zhang
Zi-xin
ZX
D001666:Binding Sites, Antibody; D015789:HLA-A2 Antigen / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008214:Lymphocytes / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D013696:Temperature
D015789:HLA-A2 Antigen; D011092:Polyethylene Glycols; C028210:monomethoxypolyethylene glycol