title: Age-associated increase of codon 72 Arginine p53 frequency in gastric cardia and non-cardia adenocarcinoma.
abstract: OBJECTIVE
  A common polymorphism of the tumor suppressor gene TP53 at codon 72 has been associated with human cancer susceptibility and prognosis. To examine the role of the polymorphism in the gastric adenocarcinoma, we examined 397 patients with or without the cancer.
  
  
  METHODS
  DNA samples were extracted from archived gastric tumor tissues and/or normal tissues of gastric adenocarcinoma and noncancer patients. The TP53 codon 72 genotypes were determined by PCR-RFLP.
  
  
  RESULTS
  The overall genotype frequencies for Pro/Pro, Arg/Pro, and Arg/Arg were 7.3, 45.1, and 47.6%, respectively. A significant stepwise increased frequency of codon 72 Arg p53 with age was observed in patients with gastric cancer, but not in noncancer patients (P = 0.01). Patients with gastric cardia cancer had a significantly higher frequency of homozygous Arg allele than those with non-cardia tumors (P = 0.03) or than noncancer patients. After adjustment for age and gender, a logistic regression analysis suggested that the risk for a p53 Arg homozygous patient to develop cardia cancer is 3.1 95% confidence interval, 1.4-7.3) times greater than for p53 Pro homozygous and p53 Arg/Pro heterozygous patients. No close relationship was observed among patient gender, tumor histological type, p53 protein expression, and codon 72 genotype distribution.
  
  
  CONCLUSIONS
  These findings indicate that codon 72 Arg p53 may be associated with a prolonged survival for patients who have had gastric adenocarcinoma, especially non-cardia adenocarcinoma. It may confer, however, a different role on patients who suffer cardia gastric adenocarcinoma.
authors.affiliation: Division of Surgery, Bristol Royal Infirmary, University of Bristol, Bristol BS2 8HW, UK. z.w.zhang@bristol.ac.uk
authors.forename: Zun-Wu
authors.initials: ZW
authors.lastname: Zhang
authors.forename: Paul
authors.initials: P
authors.lastname: Newcomb
authors.forename: Andrew
authors.initials: A
authors.lastname: Hollowood
authors.forename: Roger
authors.initials: R
authors.lastname: Feakins
authors.forename: Moganaden
authors.initials: M
authors.lastname: Moorghen
authors.forename: Alan
authors.initials: A
authors.lastname: Storey
authors.forename: Michael J G
authors.initials: MJ
authors.lastname: Farthing
authors.forename: Derek
authors.initials: D
authors.lastname: Alderson
authors.forename: Jeff
authors.initials: J
authors.lastname: Holly
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics*; D000328:Adult; D000367:Age Factors; D000368:Aged; D001120:Arginine; D002299:Cardia; D003062:Codon; D016158:Genes, p53; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000235:genetics*; D016159:Tumor Suppressor Protein p53 / Q000032:analysis
chemical_list: D003062:Codon; D016159:Tumor Suppressor Protein p53; D001120:Arginine