Effect of dehydroleucodine on histamine and serotonin release from mast cells in the isolated mouse jejunum.
OBJECTIVE
DhL, a lactone isolated from Artemisia douglasiana, prevents gastrointestinal damage elicited by necrosis-inducing agents and exhibits antiinflammatory action. This work examines the effect of DhL on compound 48/80-induced histamine and serotonin release in the isolated mouse jejunum, to determine whether DhL inhibits mediator release from mast cells at the enteric level.


METHODS
Thirty jejuna from male Balb-c mice were used for the studies.


METHODS
Samples were incubated sequentially in 9 test tubes containing RBS or 10 microg/ml compound 48/80 or 1.6 mmol/l + 10 microg/ml compound 48/80 at 37 degrees C for 90 minutes (10 min per tube).


METHODS
Histamine and serotonin release studies, quantification of granulated mast cells, and evaluation of mast cell ultrastructure were carried out. Differences between groups were determined using analysis of variance followed by Tukey-Kramer multiple comparisons test.


RESULTS
Compound 48/80 increased histamine and serotonin release by the tissue (141.95 +/- 62.58 pg/mg tissue vs basal 5.45 +/- 1.04, P<0.01 and 20.04 +/- 2.81 vs basal 9.24 +/- 1.56 ng/ mg tissue, P<0.01, respectively), decreased the number of granulated submucosal mast cells (0.077 +/- 0.0035 vs basal 0.14 +/- 0.015, P<0.05), and elicited evident granule ultrastructural changes. These effects were reduced by dehydroleucodine (19.51 +/- 7.88, P<0.01; 12.69 +/- 1, P<0.05 and 0.143 +/- 0.014, P<0.05, respectively).


CONCLUSIONS
The lactone inhibits compound 48/80-induced histamine and serotonin release from mast cells in the isolated mouse jejunum.
Penissi
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AB
Instituto de Histología y Embriología Dr. Mario H. Burgos (IHEM-CONICET), Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Casilla de Correo 56, (5500) Mendoza, Argentina. apenissi@fmed2.uncu.edu.ar
Rudolph
M I
MI
Villar
M
M
Coll
R C
RC
Fogal
T H
TH
Piezzi
R S
RS
D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D006632:Histamine / Q000378:metabolism*; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D007091:Image Processing, Computer-Assisted; D066298:In Vitro Techniques; D007583:Jejunum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D007665:Ketotifen / Q000494:pharmacology; D007783:Lactones / Q000494:pharmacology*; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D018715:Microscopy, Video; D012701:Serotonin / Q000378:metabolism*; D012717:Sesquiterpenes / Q000494:pharmacology*; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D000897:Anti-Ulcer Agents; D006634:Histamine H1 Antagonists; D007783:Lactones; D012717:Sesquiterpenes; D012701:Serotonin; C079949:dehydroleucodine; D003189:p-Methoxy-N-methylphenethylamine; D006632:Histamine; D007665:Ketotifen