Aspirin induces hepatoma-derived cell apoptosis via a hydrogen peroxide-dependent pathway.
Here we studied whether aspirin (ASA) has any influence on viability of human hepatoma-derived SKHep-1 cells and whether hydrogen peroxide (H(2)O(2)) has any relation with this effect. ASA inhibited SKHep-1 cell proliferation dose- and time-dependently. Intracellular H(2)O(2) increased as early as 15 min after ASA supplementation. Cellular apoptosis correlated with an increase in intracellular H(2)O(2) level. Moreover, in the presence of a catalase inhibitor-aminotriazol, ASA showed more apoptotic effect on SKHep-1 cells with increasing intracellular H(2)O(2) level. In conclusion, the present results shows that ASA induced SKHep-1 cell apoptosis has a relation with an early increase in intracellular H(2)O(2) level and catalase inhibitor synergizes to induce this process.
Tuvdendorj
Demidmaa
D
Faculty of Medicine, Second Department of Internal Medicine, Kagoshima University, Sakuragaoka 8 chome 35-1, Kagoshima, Japan
Oketani
Makoto
M
Ikeda
Ryuji
R
Kohara
Kazunori
K
Komorizono
Yasuzi
Y
Ishibashi
Kazuaki
K
Munkhtuvshin
Namid
N
Arima
Terukatsu
T