Usefulness of 8 kDa protein of Fasciola hepatica in diagnosis of fascioliasis.
This study was designed to detect and evaluate an antigenicity of low molecular weight proteins of Fasciola hepatica in fascioliasis. Low molecular weight protein of F. hepatica was purified by ammonium sulfate precipitation and Sephacryl S-100 HR gel filtration. The protein obtained was estimated to be 8 kDa on 7.5-15% gradient sodium dodecyl sulfate gel electrophoresis. Immunoblotting studies showed that the 8 kDa protein reacted with human fascioliasis sera, but not other trematodiasis sera. This result suggests that the 8 kDa protein of F. hepatica is one of diagnostic antigens in human fascioliasis without cross-reaction with other human trematodiasis.
Kim
Kwangsig
K
Department of General Surgery, Cheju National University Hospital, Jeju 690-716, Korea.
Yang
Hyun Jong
HJ
Chung
Young-Bae
YB
D000818:Animals; D000947:Antigens, Helminth / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D005210:Fasciola hepatica / Q000276:immunology / Q000302:isolation & purification*; D005211:Fascioliasis / Q000097:blood / Q000469:parasitology*; D015801:Helminth Proteins / Q000302:isolation & purification*; D006801:Humans; D015151:Immunoblotting
D000947:Antigens, Helminth; D015801:Helminth Proteins