title: Casein kinase ii (protein kinase ck2) regulates serotonin 5-ht(3) receptor channel function in ng108-15 cells.
abstract: We have used whole-cell recording techniques in the mouse neuroblastoma X rat glioma hybrid NG108-15 cell line to test whether protein kinase CK2 (CK2; also known as casein kinase II) modulates the function of the serotonin 5-HT(3) receptor (5-HT(3)R) channel. The rapid application of 5-HT (50 microM) to NG108-15 cells elicits a 5-HT(3)R-mediated inward current response that rapidly reaches peak amplitude and then desensitizes in the continued presence of agonist. Internal dialysis with CK2 (20 micrograms/ml) via the patch pipette significantly increases the amplitude and decreases the rate of desensitization of the 5-HT(3)R-mediated responses. CK2 that had been heat-inactivated has no effect on either the amplitude or the kinetics of desensitization of the 5-HT(3)R responses. These data suggest that dialysis with protein kinase CK2 significantly enhanced current through the 5-HT(3)R channel, and that CK2 may be an important regulator of 5-HT(3)R channel function in the nervous system, possibly serving to facilitate the 5-HT-induced excitation of the cells.
authors.affiliation: Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, P.O. Box 12233, 27709, Research Triangle Park, NC, USA.
authors.forename: S
authors.initials: S
authors.lastname: Jones
authors.forename: J L
authors.initials: JL
authors.lastname: Yakel
mesh_terms: D000818:Animals; D047390:Casein Kinase II; D002490:Central Nervous System / Q000166:cytology / Q000201:enzymology*; D007700:Kinetics; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D010766:Phosphorylation / Q000187:drug effects; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism* / Q000494:pharmacology; D051381:Rats; D011930:Reaction Time / Q000187:drug effects / Q000502:physiology; D011985:Receptors, Serotonin / Q000187:drug effects / Q000378:metabolism*; D044406:Receptors, Serotonin, 5-HT3; D012701:Serotonin / Q000378:metabolism* / Q000494:pharmacology; D017366:Serotonin Receptor Agonists / Q000494:pharmacology; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology*; D014407:Tumor Cells, Cultured
chemical_list: D011985:Receptors, Serotonin; D044406:Receptors, Serotonin, 5-HT3; D017366:Serotonin Receptor Agonists; D012701:Serotonin; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases