Characterization of digestive gland esterase-lipase activity of juvenile redclaw crayfish Cherax quadricarinatus.
Investigation of esterase-lipase activity in the digestive gland of redclaw crayfish Cherax quadricarinatus showed that the optimum enzyme activity occurred between 35 and 40 degrees C, with 100 mM NaCl at pH 8.5. Heavy metals completely inhibited and calcium ions partially inhibited enzyme activity. The enzyme activity diminished as the length of the fatty acid chain of substrates increased. Molecular masses for four isozymes were 43, 46, 63 and 118 kDa, respectively, as determined by PAGE.
López-López
Silverio
S
Laboratorio de Fisiología y Bioquímica Comparada, Centro de Investigaciones Biológicas del Noroeste, Mar Bermejo #195, Col. Playa Palo Santa Rita, La Paz, BCS 23090, Mexico.
Nolasco
Héctor
H
Vega-Villasante
Fernando
F
D000818:Animals; D003400:Astacoidea / Q000201:enzymology*; D003080:Cold Temperature; D004064:Digestive System / Q000201:enzymology*; D004950:Esterases / Q000378:metabolism*; D006358:Hot Temperature; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D050356:Lipid Metabolism; D009283:Naphthol AS D Esterase / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology
D012965:Sodium Chloride; D004950:Esterases; D009283:Naphthol AS D Esterase; D008049:Lipase; C075289:4-nitrophenylphosphorylcholine phosphodiesterase; D010727:Phosphoric Diester Hydrolases