Use of colony morphology to distinguish different enterococcal strains and species in mixed culture from clinical specimens.
Colony morphology on kanamycin esculin azide agar was investigated as a means of selecting different species and strains of enterococci from clinical specimens. Four representative colonies of each morphotype were indistinguishable by pulsed-field gel electrophoresis, biotype, and antibiogram analysis. The optimum time for identification of different colony morphotypes was 72 h.
Qamer
Shabnam
S
Department of Microbiology, University of Leeds, United Kingdom.
Sandoe
Jonathan A T
JA
Kerr
Kevin G
KG
D000362:Agar; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001386:Azides / Q000378:metabolism; D015373:Bacterial Typing Techniques; D003470:Culture Media; D016521:Electrophoresis, Gel, Pulsed-Field; D016983:Enterococcus / Q000145:classification* / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D004929:Esculin / Q000378:metabolism; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology; D006801:Humans; D007612:Kanamycin / Q000494:pharmacology; D013045:Species Specificity
D000900:Anti-Bacterial Agents; D001386:Azides; D003470:Culture Media; D004929:Esculin; D007612:Kanamycin; D000362:Agar