title: Expression of a plant-associated human cancer antigen in normal, premalignant and malignant esophageal tissues.
abstract: OBJECTIVE
  To study the relationship between the expression profiles of a plant-associated human cancer antigen and carcinogenesis of esophagus and its significance.
  
  
  METHODS
  We analyzed expression of a plant-associated human cancer antigen in biopsy specimens of normal (n=29), mildly hyperplastic (n=29), mildly (n=30), moderately (n=27) and severely dysplastic (n=29) and malignant esophageal (n=30) tissues by immunohistochemistry.
  
  
  RESULTS
  The plant-associated human cancer antigen was mainly confined to the cytoplasm and showed diffuse type of staining. Positive staining was absent or weak in normal (0/30) and mildly hyperplastic tissue samples (2/29), while strong staining was observed in severe dysplasia (23/29) and carcinoma in situ (24/30). There was significant difference of its expression between normal mucosa and severely dysplastic tissues (P<0.001) or carcinoma in situ (P<0.001). Significant difference was also observed between mild dysplasia and severe dysplasia (P<0.001) or carcinoma in situ (P<0.001). An overall trend toward increased staining intensity with increasing grade of dysplasia was found. There was a linear correlation between grade of lesions and staining intensity (r=0.794, P<0.001). Samples from esophageal cancer showed no higher levels of expression than those in severely dysplastic lesions (P>0.05).
  
  
  CONCLUSIONS
  The abnormal expression of this plant-associated human cancer antigen in esophageal lesions is a frequent and early finding in the normal-dysplasia-carcinoma sequence in esophageal carcinogenesis. It might contribute to the carcinogenesis of esophageal cancer. The abnormal expression of this plant-associated human cancer antigen in esophageal lesion tissues may serve as a potential new biomarker for early identification of esophageal cancer.
authors.affiliation: Central Laboratory for Tumor Biology, Cancer Institute, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021,China.
authors.forename: Jun
authors.initials: J
authors.lastname: Fu
authors.forename: Ping
authors.initials: P
authors.lastname: Qu
authors.forename: Mo
authors.initials: M
authors.lastname: Li
authors.forename: Hai-Mei
authors.initials: HM
authors.lastname: Tian
authors.forename: Zhen-Hai
authors.initials: ZH
authors.lastname: Zheng
authors.forename: Xin-Wen
authors.initials: XW
authors.lastname: Zheng
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000951:Antigens, Neoplasm / Q000032:analysis*; D004938:Esophageal Neoplasms / Q000276:immunology*; D004947:Esophagus / Q000276:immunology; D006801:Humans; D010944:Plants / Q000276:immunology*; D011230:Precancerous Conditions / Q000276:immunology*
chemical_list: D000951:Antigens, Neoplasm