Purification of intact plant protoplasts by flotation at 1g.
From a standard plant tissue digest adjusted to a density of 1.07 g/ml, protoplasts can be harvested by flotation through a low density barrier (1.03 g/ml). The delicate nature of these bodies is suited to this flotation strategy which can be carried out at 1g.
Graham
John M
JM
School of Biomolecular Sciences, Liverpool John Moores University. john@jgrescon.fsbusiness.co.uk
D002473:Cell Wall / Q000378:metabolism; D002480:Cellulase / Q000378:metabolism; D002499:Centrifugation, Density Gradient / Q000379:methods*; D001467:Hordeum / Q000166:cytology; D009994:Osmolar Concentration; D018515:Plant Leaves / Q000166:cytology / Q000378:metabolism; D011523:Protoplasts / Q000166:cytology*; D014283:Triiodobenzoic Acids / Q000737:chemistry
D014283:Triiodobenzoic Acids; D002480:Cellulase; C044834:iodixanol