Expression of glucocorticoid receptors in mononuclear cells in nephrotic syndrome.
Coulter flow cytometry was used to determine glucocorticoid receptors (GCR) in the peripheral blood cells of patients with nephrotic syndrome. The expression of GCR in the lymphocytes (CD3/GCR) and monocytes (CD14/GCR) of peripheral blood of 23 (age 4.9+/-2.7 years) children with steroid-sensitive nephrotic syndrome was assessed before treatment (proteinuria >50 mg/kg per 24 h), after 4-6 weeks of prednisone treatment, without proteinuria, and in remission, without proteinuria and without any treatment. Before treatment the expression of CD3/GCR was 61.8+/-18.3% and CD14/GCR 43.6.8+/-20.3%; this did not differ from the results of the normal control group ( P>0.05). However, after treatment GCR expression in lymphocytes was 50% ( P<0.001) and in monocytes about 20% lower ( P<0.05). In remission, the GCR expression increased and did not differ from the results before treatment ( P>0.05). A positive correlation between the serum cortisol concentration and the expression of CD3/GCR was found ( r=0.504, P=0.02). In summary, we report that in children with steroid-sensitive nephrotic syndrome, prednisone treatment causes the temporary decrease of the expression of GCR in lymphocytes. A positive correlation between GCR expression and serum cortisol was found. A decrease in GCR expression in monocytes did not correlate with cortisol concentration.
Wasilewska
Anna
A
1st Department of Pediatrics, Medical University Białystok, Białystok, Poland. annwasil@interia.pl
Zoch-Zwierz
Walentyna
W
Tomaszewska
Barbara
B
Wierciński
Ryszard
R
Stasiak-Barmuta
Anna
A
D000293:Adolescent; D017252:CD3 Complex / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry; D005938:Glucocorticoids / Q000008:administration & dosage; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008297:Male; D009404:Nephrotic Syndrome / Q000188:drug therapy / Q000276:immunology* / Q000378:metabolism*; D011239:Prednisolone / Q000008:administration & dosage; D011965:Receptors, Glucocorticoid / Q000378:metabolism*
D017252:CD3 Complex; D005938:Glucocorticoids; D018950:Lipopolysaccharide Receptors; D011965:Receptors, Glucocorticoid; D011239:Prednisolone; D006854:Hydrocortisone