title: Microbial production of 2-deoxyribose 5-phosphate from acetaldehyde and triosephosphate for the synthesis of 2'-deoxyribonucleosides.
abstract: 2-Deoxyribose 5-phosphate was produced from acetaldehyde and dihydroxyacetone phosphate via D-glyceraldehyde 3-phosphate by Klebsiella pneumoniae B-4-4 through deoxyriboaldolase- and triosephosphate isomerase-catalyzing reactions. Under the optimum conditions, 98.7 mM 2-deoxyribose 5-phosphate was produced from 200 mM acetaldehyde and 117 mM dihydroxyacetone phosphate in 2 h with a molar yield of 84%. The 2-deoxyriobse 5-phosphate produced was directly transformed to 2'-deoxyribonucleoside by phosphopentomutase- and nucleoside phosphorylase-catalyzing reactions.
authors.affiliation: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Ogawa
authors.forename: Kyota
authors.initials: K
authors.lastname: Saito
authors.forename: Takafumi
authors.initials: T
authors.lastname: Sakai
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Horinouchi
authors.forename: Takako
authors.initials: T
authors.lastname: Kawano
authors.forename: Seiichiro
authors.initials: S
authors.lastname: Matsumoto
authors.forename: Mie
authors.initials: M
authors.lastname: Sasaki
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Mikami
authors.forename: Sakayu
authors.initials: S
authors.lastname: Shimizu
mesh_terms: D000079:Acetaldehyde / Q000378:metabolism; D003853:Deoxyribonucleosides / Q000096:biosynthesis*; D007711:Klebsiella pneumoniae / Q000378:metabolism*; D010430:Pentosyltransferases / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism; D012267:Ribosemonophosphates / Q000096:biosynthesis*; D013403:Sugar Phosphates / Q000378:metabolism; D014306:Trioses / Q000378:metabolism
chemical_list: D003853:Deoxyribonucleosides; D012267:Ribosemonophosphates; D013403:Sugar Phosphates; D014306:Trioses; C058245:2-deoxyribose 5-phosphate; D010430:Pentosyltransferases; C067995:nucleoside phosphorylase; D010770:Phosphotransferases; C022375:phosphopentomutase; D000079:Acetaldehyde