title: Activity of coliphage HK022 excisionase (Xis) in the absence of DNA binding.
abstract: A mutated excisionase (Xis) protein of coliphage HK022 whose single Cys residue was replaced by Ser does not bind to its two tandem binding sites (X1, X2) on the P arm of attR. Despite its DNA-binding inability the protein showed 30% excision activity of the wild type Xis both in vitro and in vivo. This partial activity is attributed to the interaction of Xis with integrase that is retained in the mutant protein. This protein-protein interaction occurs in the absence of DNA binding.
authors.affiliation: Department of Biochemistry, Tel-Aviv University, Tel-Aviv 69978, Israel.
authors.forename: Pnina
authors.initials: P
authors.lastname: Gottfried
authors.forename: Nava
authors.initials: N
authors.lastname: Silberstein
authors.forename: Ezra
authors.initials: E
authors.lastname: Yagil
authors.forename: Mikhail
authors.initials: M
authors.lastname: Kolot
mesh_terms: D025523:Bacteriophage HK022 / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D004254:DNA Nucleotidyltransferases / Q000235:genetics* / Q000378:metabolism*; D004279:DNA, Viral / Q000235:genetics; D004268:DNA-Binding Proteins / Q000378:metabolism; D019426:Integrases / Q000378:metabolism; D008969:Molecular Sequence Data; D017354:Point Mutation; D017434:Protein Structure, Tertiary; D011995:Recombination, Genetic; D012694:Serine / Q000378:metabolism; D014764:Viral Proteins
chemical_list: D004279:DNA, Viral; D004268:DNA-Binding Proteins; D014764:Viral Proteins; D012694:Serine; D004254:DNA Nucleotidyltransferases; D019426:Integrases; C063911:excisionase