title: Correlates of IL-10 and IL-12 concentrations in cervical secretions.
abstract: Interindividual variations in host immune responses to HPV infection are thought to be important determinants of viral persistence and progression to cervical intraepithelial neoplasia and cancer. However, few studies have measured local immune markers at the site of infection (e.g., the cervical mucosa). We sought to determine biologic correlates of IL-10 and IL-12 concentrations in cervical secretions. Cervical secretions were passively collected using a WeckCel sponge from 247 women participating in a natural history study of human papillomavirus infection as part of an immunologic ancillary study. IL-10 and IL-12 concentrations were determined using standard ELISA assays. In general, IL-10 and IL-12 levels were significantly intercorrelated (Pearson's correlation coefficient = 0.6) but had somewhat different determinants. Significant increases (P < 0.05) in IL-10 concentrations were observed for nonovulatory phases of the menstrual cycle, postmenopausal status, recent use of oral contraceptives (OC), low secretion volume, macrolevels of heme contamination, and high vaginal pH. Increasing IL-10 levels were also observed among smokers, women with increasing numbers of lifetime sex partners, and women who report having less frequent sex (less than once per week), however, these results were not statistically significant. Significantly higher IL-12 concentrations were observed among recent OC users, women with low secretion volume, and women with a high vaginal pH. There was a non-statistically significant observation of increasing IL-12 levels among nonsmokers, women with increasing numbers of lifetime and recent pregnancies, and increasing levels of heme contamination. We failed to observe a significant association between HPV and IL-10 or IL-12 levels in this crosssectional sample. Future analyses of cervical cytokine levels and HPV infection should control for the inherent variation of local cytokine levels due to hormonal influences, hemoglobin contamination, pH, and cervical secretion volume differences.
authors.affiliation: Interdisciplinary Studies Section, Environmental Epidemiology Branch, DCEG, National Cancer Institute, 6120 Executive Blvd, EPS 7059, Bethesda, Maryland 20852, USA.
authors.forename: Patti E
authors.initials: PE
authors.lastname: Gravitt
authors.forename: Allan
authors.initials: A
authors.lastname: Hildesheim
authors.forename: Rolando
authors.initials: R
authors.lastname: Herrero
authors.forename: Mark
authors.initials: M
authors.lastname: Schiffman
authors.forename: Mark E
authors.initials: ME
authors.lastname: Sherman
authors.forename: M Concepcion
authors.initials: MC
authors.lastname: Bratti
authors.forename: Ana C
authors.initials: AC
authors.lastname: Rodriguez
authors.forename: Lidia A
authors.initials: LA
authors.lastname: Morera
authors.forename: Fernando
authors.initials: F
authors.lastname: Cardenas
authors.forename: Frederick P
authors.initials: FP
authors.lastname: Bowman
authors.forename: Keerti V
authors.initials: KV
authors.lastname: Shah
authors.forename: Peggy A
authors.initials: PA
authors.lastname: Crowley-Nowick
mesh_terms: D000328:Adult; D002582:Cervix Mucus / Q000737:chemistry* / Q000276:immunology; D002584:Cervix Uteri / Q000276:immunology / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000032:analysis*; D018664:Interleukin-12 / Q000032:analysis*; D009092:Mucous Membrane / Q000276:immunology / Q000378:metabolism; D027383:Papillomaviridae; D030361:Papillomavirus Infections / Q000276:immunology
chemical_list: D016753:Interleukin-10; D018664:Interleukin-12