Monitoring DNA hybridization on alkyl modified silicon surface through capacitance measurement.
Single strand oligodeoxynucleotide is attached to the alkyl modified silicon surface through a peptide bond. The oligodeoxynucleotide-modified silicon substrate is used as a working electrode in an electrochemical cell system. After the electrode is treated by a solution containing strands of complementary oligodeoxynucleotide the Mott-Schottky measurements exhibit obvious negative shift in the flat band potential of the electrode, while in a control experiment treated with a solution of non-complementary oligodeoxynucleotide such a shift does not occur. The DNA hybridization is also manifested in a real time capacitance measurement. A DNA sensor based on the capacitance measurement could be more convenient than that based on a fluorescence detection.
Wei
Fang
F
State Key Laboratory of Molecular Dynamic and Stable Structures, and Department of Chemical Biology, College of Chemistry, Peking University, 100871, Beijing, China.
Sun
Bin
B
Guo
Yuan
Y
Zhao
Xin Sheng
XS
D000478:Alkylation; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D015342:DNA Probes; D025802:Electric Capacitance; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D009841:Oligonucleotides; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D016415:Sequence Alignment / Q000295:instrumentation / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000295:instrumentation / Q000379:methods*; D012689:Sequence Homology, Nucleic Acid; D012825:Silicon
D015342:DNA Probes; D009841:Oligonucleotides; D004247:DNA; D012825:Silicon