Effects of two vitamins, two growth factors and dexamethasone on the proliferation of rat bone marrow stromal cells and osteoblastic MC3T3-E1 cells.
The purpose of this investigation was to examine the effects of the addition of five cytokines such as vitamin C, vitamin D, bone morphogenetic protein (BMP), transforming growth factor-beta (TGF-beta) and dexamethasone (Dex) to Dulbecco's modified Eagle (DME) medium on the proliferation of Sprague-Dawley (SD) rats' bone marrow stromal cells and osteoblastic MC3T3-E1 cells by conducting 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. For bone marrow stromal cells, vitamin C was needed for doubling the cell viability. While BMP, TGF-beta and vitamin D maintained the growth rate given by vitamin C, Dex with beta-glycerophosphate (beta-GP) slightly reduced this cell proliferation rate. For MC3T3-E1 cells, the addition of four cytokines examined (vitamin C, vitamin D, TGF-beta and BMP) did not significantly increase the cell proliferation while Dex with vitamin C and beta-GP slightly enlarged the cell proliferation. It can be pointed out that vitamin C in DME medium is indispensable for rapid proliferation of bone marrow stromal cells that contain many osteo-progenitor cells but is not effective for quick increase of osteoblastic MC3T3-E1 cells. This finding appears to contribute to tissue engineering therapy to fix bone and periodontal defects in dentistry.
Taira
M
M
Department of Dental Materials Science and Technology, Iwate Medical University School of Dentistry, Iwate, Japan. mtaira@iwate-med.ac.jp
Nakao
H
H
Takahashi
J
J
Araki
Y
Y
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D016207:Cytokines / Q000494:pharmacology*; D003907:Dexamethasone / Q000494:pharmacology*; D005938:Glucocorticoids / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects*; D016212:Transforming Growth Factor beta / Q000032:analysis; D014807:Vitamin D / Q000494:pharmacology
D016207:Cytokines; D005938:Glucocorticoids; D016212:Transforming Growth Factor beta; D014807:Vitamin D; D003907:Dexamethasone; D001205:Ascorbic Acid