Antioxidants prevent UV-induced apoptosis by inhibiting mitochondrial cytochrome c release and caspase activation in Spodoptera frugiperda (Sf9) cells.
Oxidative stress has been shown to be associated with apoptosis (programmed cell death) in a number of cell systems. We earlier reported in vitro cultured Spodoptera frugiperda (Sf9) cells as a model system to study oxidative stress induced apoptosis (J Biosciences 24 (1999) 13) and the inhibition of UV-induced apoptosis by the baculovirus antiapoptotic p35 protein that acts as a sink to sequester reactive oxygen species (Proc Natl Acad Sci USA 96 (1999) 4838). We now show that UV-induced apoptosis in Sf9 cells, is preceded by the release of mitochondrial cytochrome c into the cytosol and consequent activation of Sf-caspase-1. The inhibitory effect of different antioxidants including scavengers of oxygen radicals such as butylated hydroxyanisole (BHA), alpha tocopherol acetate, benzoate and reduced-glutathione (GSH) on ultra violet B (UVB)-induced apoptosis in cultured Sf9 cells was assessed. Both, cytochrome c release as well as Sf-caspase-1 activation was inhibited by pre-treatment with antioxidants such as BHA and alpha tocopherol acetate, suggesting that these antioxidants inhibit apoptosis by acting quite upstream in the apoptosis cascade at the mitochondrial level, as well as downstream at the caspase level.
Mohan
Manjari
M
Laboratory of Molecular and Cellular Biology, Centre for DNA Fingerprinting and Diagnostics, Hyderabad-500076, India.
Taneja
Tarvinder K
TK
Sahdev
Sudhir
S
Mohareer
Krishnaveni
K
Begum
Rasheedunnisa
R
Athar
Mohammad
M
Sah
Nand K
NK
Hasnain
Seyed E
SE
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D020170:Caspase 1 / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D020169:Caspases / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D002478:Cells, Cultured; D045304:Cytochromes c / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects / Q000528:radiation effects; D016166:Free Radical Scavengers / Q000494:pharmacology; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology* / Q000528:radiation effects; D008954:Models, Biological; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D018411:Spodoptera; D014466:Ultraviolet Rays
D000975:Antioxidants; D016166:Free Radical Scavengers; D045304:Cytochromes c; D020169:Caspases; D020170:Caspase 1