[A study on introduction of chitinase gene and beta-1,3-glucanase gene into restorer line of dian-type hybrid rice (Oryza sativa L.) and enhanced resistance to blast (Magnaporthe grisea)].
Plasmid pBLGC containing chitinase gene from Phaseolus limensis and beta-1,3-glucanase gene from Nicotiana tabacum was bombarded into the restorer line "Nan29" of Dian-type hybrid rice (Oryza sativa L. ssp. japonica) from Yunnan province of South-west China. 93 regenerants were obtained from the calli that were resistant to G418 (100 to 150 mg/L) on NB medium. Using beta-1,3 glucanase gene as the probe, 17 of the regenerants were identified to be transgenic lines by dot blotting and the foreign genes construction were integrated into the genomes of T1 lines by Southern blotting hybridization. Two foreign genes were inherited stably to T4 generation according to PCR results of the lines. The resistance to rice blast of six transgenic lines were evaluated by inoculating four violent biological races of Magnaporthe grisea from Yunnan province and inducing the disease in the field. The results indicated that the resistance to rice blast of transgenic lines were enhanced to varying degrees compared with the receptor line and the transgenic lines could be used in rice blast resistant breeding.
Xu
Ming-Hui
MH
Institute of Biotechnology, Yunnan Academy of Agricultural Sciences, Kunming 650223, China. xuminghui@sohu.com
Tang
Zuo-Shun
ZS
Tan
Ya-Ling
YL
Tian
Ying-Chuan
YC
Li
Cheng-Yun
CY
Zhang
Shu-Hua
SH
Chen
Zheng-Hua
ZH
Tian
Wen-Zhong
WZ
D015139:Blotting, Southern; D002688:Chitinases / Q000235:genetics*; D046508:Culture Techniques; D018744:DNA, Plant / Q000235:genetics; D043326:Glucan 1,3-beta-Glucosidase / Q000235:genetics*; D006824:Hybridization, Genetic; D007113:Immunity, Innate / Q000235:genetics; D020082:Magnaporthe / Q000254:growth & development*; D012275:Oryza / Q000235:genetics* / Q000382:microbiology / Q000502:physiology; D027805:Phaseolus / Q000201:enzymology; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000382:microbiology / Q000502:physiology; D016133:Polymerase Chain Reaction; D012038:Regeneration / Q000235:genetics; D014026:Nicotiana / Q000201:enzymology
D018744:DNA, Plant; D002688:Chitinases; D043326:Glucan 1,3-beta-Glucosidase