title: The S haplotypes lacking SLG in the genome of Brassica rapa.
abstract: Self-incompatibility (SI) discriminating self- and non-self pollen is regulated by S-locus genes in Brassica. In most of the S haplotypes, a highly polymorphic S-locus glycoprotein ( SLG) gene is tightly linked to genes for the SI determinants, S-receptor kinase ( SRK) and SP11, although the precise function of SLG in SI has not been clarified. In the present study, we performed DNA gel blot analysis for S(32), S(33), and S(36) haplotypes of Brassica rapa showing normal SI phenotypes and concluded that there might be no SLG in their genome. RNA gel blot analysis of the SLG-less S haplotypes indicated the possible existence of eSRK transcripts in the stigma. These three S haplotypes are useful resources to discern the molecular mechanism of the SI reaction without SLG.
authors.affiliation: Division of Natural Science, Osaka Kyoiku University, Kashiwara, 582-8582, Osaka, Japan.
authors.forename: G
authors.initials: G
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Kakizaki
authors.forename: Y
authors.initials: Y
authors.lastname: Takada
authors.forename: H
authors.initials: H
authors.lastname: Shiba
authors.forename: S
authors.initials: S
authors.lastname: Takayama
authors.forename: A
authors.initials: A
authors.lastname: Isogai
authors.forename: M
authors.initials: M
authors.lastname: Watanabe
mesh_terms: D001937:Brassica / Q000235:genetics*; D018745:Genome, Plant; D006023:Glycoproteins / Q000235:genetics*; D006239:Haplotypes; D010940:Plant Proteins / Q000235:genetics*
chemical_list: D006023:Glycoproteins; D010940:Plant Proteins; C064156:self-incompatibility locus specific glycoprotein, Brassica