Increased expression of Hsp70 for resistance to deuterium oxide in a yeast mutant cell line.
Labeling with stable isotopes, typically deuterium (D), is powerful tool for studying the functional structure of biomolecules by NMR. Biosynthesis of certain deuterated proteins in microorganisms cultured in deuterium oxide (D(2)O) is an attractive strategy. However, the growth of almost all microorganisms is inhibited at high concentrations of D(2)O. We isolated a mutant of yeast that grows well in D(2)O. The expression of Hsp70 was enhanced in the mutant. The increased expression also endowed the yeast with cold-resistance. The mutant might be useful for biosynthesis of D-labeled biomolecules.
Unno
Keiko
K
Department of Medical Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Shizuoka 422-8526, Japan. unno@u-shizuoka-ken.ac.jp
Kishido
Takahiro
T
Morioka
Mizue
M
Okada
Shoji
S
Oku
Naoto
N
D001483:Base Sequence; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003001:Cloning, Molecular; D003080:Cold Temperature; D017666:Deuterium Oxide / Q000494:pharmacology*; D025141:Drug Resistance, Fungal; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D007553:Isotope Labeling; D008969:Molecular Sequence Data; D009154:Mutation; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics
D018840:HSP70 Heat-Shock Proteins; D029701:Saccharomyces cerevisiae Proteins; D017666:Deuterium Oxide