title: Inhibitory effect of serum globulins on the adhesion of Prevotella nigrescens to hydroxyapatite.
abstract: Prevotella nigrescens ATCC 25261 cells adhere well to protein-blocking hydroxyapatite (HA) which mimics a root surface in the periodontal pocket treated with proteases such as trypsin, proteinase K, chymotrypsin and papain. This study was done to clarify the inhibitory effect of alpha- and beta- serum globulins on the adhesion of P. nigrescens ATCC 25261 cells to trypsin-treated HA. The inhibitory effect was found to be caused by the alpha1-antitrypsin (alpha1-AT) of alpha-globulin and the low-density lipoprotein (LDL) of beta-globulin under experimental conditions. The most effective inhibition of alpha1-AT on P. nigrescens ATCC 25261 cell attachment to HA was achieved when alpha1-AT-treated trypsin was used. The most effective inhibition of LDL occurred when trypsin-treated HA was treated with LDL. Apo-transferrin (TF) and holo-TF, which are beta-globulins, did not affect the attachment of P. nigrescens ATCC 25261 cells to trypsin-treated HA.
authors.affiliation: Department of Bacteriology, Nihon University School of Dentistry, Tokyo 101-8310, Japan. kuroda-k@dent.nihon-u.ac.jp
authors.forename: Kouichiro
authors.initials: K
authors.lastname: Kuroda
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Hirano
authors.forename: Muneaki
authors.initials: M
authors.lastname: Tamura
authors.forename: Kunio
authors.initials: K
authors.lastname: Hayashi
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects*; D002417:Cattle; D017886:Durapatite; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D018720:Prevotella / Q000502:physiology*; D012712:Serum Globulins / Q000494:pharmacology*; D014357:Trypsin / Q000494:pharmacology; D000515:alpha 1-Antitrypsin / Q000494:pharmacology
chemical_list: D008077:Lipoproteins, LDL; D012712:Serum Globulins; D000515:alpha 1-Antitrypsin; D017886:Durapatite; D014357:Trypsin