Predictive value of sperm fluorescence in situ hybridization analysis on the outcome of preimplantation genetic diagnosis for translocations.
OBJECTIVE
To determine whether the proportion of abnormal sperm is predictive of the proportion of abnormal embryos from couples in which the males are translocation carriers.


METHODS
Controlled clinical study.


METHODS
Private in vitro fertilization (IVF) center.


METHODS
Eleven cases of reciprocal translocation male carriers.


METHODS
Blood sample and sperm sample collection from each male partner. Embryo biopsy of the embryos produced in each cycle.


METHODS
Fluorescence in situ hybridization on lymphocyte slides to characterize each translocation case, then fluorescent in situ hybridization (FISH) with specific probes for each of the sperm samples. Preimplantation genetic diagnosis of the translocations in the 11 cases.


RESULTS
A correlation was found between the percentage of abnormal gametes and the percentage of abnormal embryos, and a predictive equation is proposed for this relationship: A = -55 + (1.9 x B), where A is the percentage of abnormal embryos and B the percentage of abnormal sperm.


CONCLUSIONS
The predictive value of the sperm analysis was established. Patients with 65% or less chromosomally abnormal sperm have a good chance at conceiving; patients with higher rates would need to produce 10 or more good quality embryos to have reasonable chances of conceiving.
Escudero
Tomas
T
The Institute for Reproductive Medicine and Science, Saint Barnabas Medical Center, West Orange, New Jersey 07052, USA.
Abdelhadi
Iman
I
Sandalinas
Mireia
M
Munné
Santiago
S
D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D011247:Pregnancy; D019836:Preimplantation Diagnosis / Q000379:methods*; D013094:Spermatozoa / Q000648:ultrastructure*; D016615:Telomere; D014178:Translocation, Genetic