Diamond-type lipid cubic phase with large water channels.
This paper describes a diamond cubic phase with large water channels and determines the temperature dependence of the bilayer thickness in the cubic monoolein/octylglucoside/water system based on time-resolved synchrotron X-ray diffraction data. The X-ray diffraction study established a diamond-type lipid cubic phase with large water channels (Dlarge), which has not been previously reported. It is a distinct phase, different from the diamond cubic phase with normal water channels (Dnormal). The larger channels might allow an enhanced entrapment efficiency of biomolecules in lipid cubic phases. The X-ray diffraction patterns recorded during a thermal scan showed a cubic-cubic structural transition from Dlarge to Dnormal. The obtained cubic phases displayed much larger lattice spacings as compared to those of pure monoolein at full hydration.
Angelov
Borislav
B
Institute of Biophysics, Bulgarian Academy of Sciences, Acad. G.Bonchev Str. Bl.21, BG-1113 Sofia, Bulgaria.
Angelova
Angelina
A
Ollivon
Michel
M
Bourgaux
Claudie
C
Campitelli
Andrew
A
D005960:Glucosides / Q000737:chemistry*; D005989:Glycerides / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D012542:Scattering, Radiation; D013696:Temperature; D014867:Water / Q000737:chemistry*; D014961:X-Ray Diffraction; D014965:X-Rays
D005960:Glucosides; D005989:Glycerides; D008051:Lipid Bilayers; D014867:Water; C018619:octyl-beta-D-glucoside; C005953:monoolein