Self-assembling protein arrays using electronic semiconductor microchips and in vitro translation.
Protein arrays will greatly accelerate research and development in medical and biological sciences. We have used cell-free protein biosynthesis and a parallel immobilization strategy for producing protein biochips. We demonstrate a model two-protein microarray using luciferase and green fluorescent protein, both expressed in a cell-free system and specifically immobilized on CombiMatrix semiconductor oligonucleotide microarrays. This demonstration provides evidence for the appropriate folding, activity, robust presentation, and efficient flexible detection of proteins on the microscale.
Oleinikov
Andrew V
AV
CombiMatrix Corporation, 6500 Harbor Heights Pkwy, Suite 301, Mukilteo, Washington 98275, USA. avolein@combimatrix.com
Gray
Matthew D
MD
Zhao
Jun
J
Montgomery
Donald D
DD
Ghindilis
Andrey L
AL
Dill
Kilian
K
D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D040081:Protein Array Analysis / Q000379:methods*; D014176:Protein Biosynthesis; D012666:Semiconductors; D013194:Staining and Labeling