Vaccine-induced immunity in baboons by using DNA and replication-incompetent adenovirus type 5 vectors expressing a human immunodeficiency virus type 1 gag gene.
The cellular immunogenicity of formulated plasmid DNA and replication-defective human adenovirus serotype 5 (Ad5) vaccine vectors expressing a codon-optimized human immunodeficiency virus type 1 gag gene was examined in baboons. The Ad5 vaccine was capable of inducing consistently strong, long-lived CD8(+)-biased T-cell responses and in vitro cytotoxic activities. The DNA vaccine-elicited immune responses were weaker than those elicited by the Ad5 vaccine and highly variable; formulation with chemical adjuvants led to moderate increases in the levels of Gag-specific T cells. Increasing the DNA-primed responses with booster doses of either Ad5 or modified vaccinia virus Ankara vaccines suggests a difference in the relative levels of cytotoxic and helper responses. The implications of these results are discussed.
Casimiro
Danilo R
DR
Department of Viral Vaccine Research, Merck Research Laboratories, West Point, Pennsylvania 19486, USA. danilo_casimiro@merck.com
Tang
Aimin
A
Chen
Ling
L
Fu
Tong-Ming
TM
Evans
Robert K
RK
Davies
Mary-Ellen
ME
Freed
Daniel C
DC
Hurni
William
W
Aste-Amezaga
Jose M
JM
Guan
Liming
L
Long
Romnie
R
Huang
Lingyi
L
Harris
Virginia
V
Nawrocki
Denise K
DK
Mach
Henryk
H
Troutman
Robert D
RD
Isopi
Lynne A
LA
Murthy
Krishna K
KK
Rice
Karen
K
Wilson
Keith A
KA
Volkin
David B
DB
Emini
Emilio A
EA
Shiver
John W
JW
D016915:AIDS Vaccines / Q000008:administration & dosage / Q000276:immunology*; D000256:Adenoviridae / Q000235:genetics* / Q000276:immunology; D000818:Animals; D003673:Defective Viruses / Q000235:genetics* / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D015750:Genes, gag; D015497:HIV-1 / Q000235:genetics*; D010215:Papio; D013601:T-Lymphocytes / Q000276:immunology
D016915:AIDS Vaccines