Subcellular distribution of ryanodine receptors in the cardiac muscle of carp (Cyprinus carpio).
We examined the subcellular localization of ryanodine receptors (RyR) in the cardiac muscle of carp using biochemical, immunohistochemical, and electron microscopic methods and compared it with those of rats and guinea pigs. To achieve this goal, an anti-RyR antibody was newly raised against a synthetic peptide corresponding to an amino acid sequence that was conserved among all sequenced RyRs. Western blot analysis using this antibody detected a single RyR band following the SDS-PAGE of sarcoplasmic reticulum (SR) membranes from carp atrium and ventricle as well as from mammalian hearts and skeletal muscles. The carp heart band had slightly greater mobility than those of mammalian hearts. Although immunohistochemical staining showed evident striations corresponding to the Z lines in longitudinal sections of mammalian hearts, clusters of punctate staining, in contrast, were distributed ubiquitously throughout carp atrium and ventricle. Electron microscopic images of the carp myocardium showed that the SR was observed largely as the subsarcolemmal cisternae and the reticular SR, suggesting that the RyR is localized in the junctional and corbular SR.
Chugun
Akihito
A
Dept. of Pharmacology, School of Medicine, Juntendo Univ., Hongo 2-1-1, Bunkyo-ku, Tokyo 113-8421, Japan. chugun@med.juntendo.ac.jp
Taniguchi
Kazumi
K
Murayama
Takashi
T
Uchide
Tsuyoshi
T
Hara
Yukio
Y
Temma
Kyosuke
K
Ogawa
Yasuo
Y
Akera
Tai
T
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D015153:Blotting, Western; D002347:Carps / Q000502:physiology*; D005260:Female; D006168:Guinea Pigs; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009206:Myocardium / Q000737:chemistry* / Q000166:cytology; D032383:Myocytes, Cardiac / Q000737:chemistry / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D019837:Ryanodine Receptor Calcium Release Channel / Q000032:analysis* / Q000235:genetics* / Q000276:immunology; D012519:Sarcoplasmic Reticulum / Q000737:chemistry
D000906:Antibodies; D019837:Ryanodine Receptor Calcium Release Channel