title: In vivo port-wine stain depth determination with a photoacoustic probe.
abstract: We have designed a photoacoustic probe for port-wine stain (PWS) depth measurements consisting of optical fibers for laser light delivery and a piezoelectric element for acoustic detection. We characterized the capabilities and limitations of the probe for profiling PWS skin. The probe induced and measured photoacoustic waves in acrylamide tissue phantoms and PWS skin in vivo. The optical properties of the phantoms were chosen to mimic those of PWS skin. We denoised acoustic waves using spline wavelet transforms, then deconvolved with the impulse response of the probe to yield initial subsurface pressure distributions in phantoms and PWS skin. Using the phantoms, we determined that the limit in resolving epidermal and PWS layers was less than 70 microm. In addition, we used the phantoms to determine that the maximum epidermal melanin concentration that allowed detection of PWS was between 13 and 20%. In vivo measurements of PWS skin with different epidermal melanin concentrations correlated with the phantoms. Thus the photoacoustic probe can be used to determine PWS depth for most patients receiving laser therapy.
authors.affiliation: Beckman Laser Institute, University of California, Irvine, 1002 Health Sciences Road East, Irvine, Calfornia 92612, USA. javiator@laser.bli.uci.edu
authors.forename: John A
authors.initials: JA
authors.lastname: Viator
authors.forename: Bernard
authors.initials: B
authors.lastname: Choi
authors.forename: Martin
authors.initials: M
authors.lastname: Ambrose
authors.forename: Jerome
authors.initials: J
authors.lastname: Spanier
authors.forename: J Stuart
authors.initials: JS
authors.lastname: Nelson
mesh_terms: D000162:Acoustics; D004864:Equipment and Supplies; D006801:Humans; D008543:Melanins / Q000378:metabolism; D055095:Optics and Photonics; D019047:Phantoms, Imaging; D019339:Port-Wine Stain / Q000175:diagnosis*; D012867:Skin / Q000378:metabolism
chemical_list: D008543:Melanins