Effects of anti-allergic drugs on intestinal mastocytosis and worm expulsion of rats infected with Neodiplostomum seoulense.
The effects of anti-allergic drugs on intestinal mastocytosis and the expulsion of Neodiplostomum seoulense were observed in Sprague-Dawley rats, after oral infection with 500 metacercariae. The drugs used were hydroxyzine (a histamine receptor H1 blocker), cimetidine (a H2 blocker), cyclosporin-A (a helper T-cell suppressant), and prednisolone (a T- and B-cell suppressant). Infected, but untreated controls, and uninfected controls, were prepared. Worm recovery rate and intestinal mastocytosis were measured on weeks 1, 2, 3, 5, and 7 post-infection. Compared with the infected controls, worm expulsion was significantly (P < 0.05) delayed in hydroxyzine- and cimetidine-treated rats, despite mastocytosis being equally marked in the duodenum of all three groups. In the cyclosporin-A- and prednisolone-treated groups, mastocytosis was suppressed, but worm expulsion was only slightly delayed, without statistical significance. Our results suggest that binding of histamine to its receptors on intestinal smooth muscles is more important in terms of the expulsion of N. seoulense from rats than the levels of histamine alone, or mastocytosis.
Shin
Eun-Hee
EH
Department of Parasitology and Tropical Medicine, College of Medicine and Institute of Endemic Diseases, Medical Research Center, Seoul National University, Seoul 110-799, Korea.
Kim
Tae-Heung
TH
Hong
Sung-Jong
SJ
Park
Jae-Hwan
JH
Guk
Sang-Mee
SM
Chai
Jong-Yil
JY
D000818:Animals; D002927:Cimetidine / Q000494:pharmacology; D016572:Cyclosporine / Q000494:pharmacology; D006634:Histamine H1 Antagonists / Q000494:pharmacology*; D006635:Histamine H2 Antagonists / Q000494:pharmacology*; D006919:Hydroxyzine / Q000494:pharmacology / Q000627:therapeutic use; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007411:Intestinal Diseases, Parasitic / Q000188:drug therapy*; D008415:Mastocytosis / Q000188:drug therapy* / Q000469:parasitology; D011239:Prednisolone / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014200:Trematoda / Q000254:growth & development* / Q000378:metabolism; D014201:Trematode Infections / Q000188:drug therapy*
D006634:Histamine H1 Antagonists; D006635:Histamine H2 Antagonists; D007166:Immunosuppressive Agents; D006919:Hydroxyzine; D002927:Cimetidine; D016572:Cyclosporine; D011239:Prednisolone