title: Aspirin induces hepatoma-derived cell apoptosis via a hydrogen peroxide-dependent pathway.
abstract: Here we studied whether aspirin (ASA) has any influence on viability of human hepatoma-derived SKHep-1 cells and whether hydrogen peroxide (H(2)O(2)) has any relation with this effect. ASA inhibited SKHep-1 cell proliferation dose- and time-dependently. Intracellular H(2)O(2) increased as early as 15 min after ASA supplementation. Cellular apoptosis correlated with an increase in intracellular H(2)O(2) level. Moreover, in the presence of a catalase inhibitor-aminotriazol, ASA showed more apoptotic effect on SKHep-1 cells with increasing intracellular H(2)O(2) level. In conclusion, the present results shows that ASA induced SKHep-1 cell apoptosis has a relation with an early increase in intracellular H(2)O(2) level and catalase inhibitor synergizes to induce this process.
authors.affiliation: Faculty of Medicine, Second Department of Internal Medicine, Kagoshima University, Sakuragaoka 8 chome 35-1, Kagoshima, Japan
authors.forename: Demidmaa
authors.initials: D
authors.lastname: Tuvdendorj
authors.forename: Makoto
authors.initials: M
authors.lastname: Oketani
authors.forename: Ryuji
authors.initials: R
authors.lastname: Ikeda
authors.forename: Kazunori
authors.initials: K
authors.lastname: Kohara
authors.forename: Yasuzi
authors.initials: Y
authors.lastname: Komorizono
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Ishibashi
authors.forename: Namid
authors.initials: N
authors.lastname: Munkhtuvshin
authors.forename: Terukatsu
authors.initials: T
authors.lastname: Arima