Phospholipase A2 hydrolysis of mixed phospholipid vesicles formed on polyelectrolyte hollow capsules.
Mixtures of the phospholipids L-alpha-dimyristoylphosphatidic acid (DMPA) and L-alpha-dipalmitoylphosphatidylcholine (DPPC) have been successfully adsorbed onto the charged surface of multilayer polyelectrolyte capsules to form a novel vesicle. Leaving such vesicles in phospholipase A(2) solution, we observed the hydrolysis reaction on the surface of the lipid/polymer vesicles and a permeability change before and after the reaction by confocal-laser scanning microscopy (CLSM). A capsule with adjustable permeability was constructed. This method may provide new features for drug-release vesicles.
Ge
Liqin
L
International Joint Lab, Key Lab of Colloid and Interface Science, The Center for Molecular Science, Institute of Chemistry, Chinese Academy of Science, Beijing 100080, China.
Möhwald
Helmuth
H
Li
Junbai
J
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry*; D000327:Adsorption; D002214:Capsules; D004573:Electrolytes / Q000737:chemistry*; D020404:Glycerophospholipids / Q000737:chemistry*; D006868:Hydrolysis; D008823:Micelles; D018613:Microscopy, Confocal; D010539:Permeability; D010741:Phospholipases A / Q000737:chemistry*; D054467:Phospholipases A2; D013499:Surface Properties
D002214:Capsules; D004573:Electrolytes; D020404:Glycerophospholipids; D008823:Micelles; D015060:1,2-Dipalmitoylphosphatidylcholine; C025602:dimyristoylphosphatidic acid; D010741:Phospholipases A; D054467:Phospholipases A2