Automated data processing of [1H-decoupled] 13C MR spectra acquired from human brain in vivo.
In clinical 13C infusion studies, broadband excitation of 200 ppm of the human brain yields 13C MR spectra with a time resolution of 2-5 min and generates up to 2000 metabolite peaks over 2h. We describe a fast, automated, observer-independent technique for processing [1H-decoupled] 13C spectra. Quantified 13C spectroscopic signals, before and after the administration of [1-13C]glucose and/or [1-13C]acetate in human subjects are determined. Stepwise improvements of data processing are illustrated by examples of normal and pathological results. Variation in analysis of individual 13C resonances ranged between 2 and 14%. Using this method it is possible to reliably identify subtle metabolic effects of brain disease including Alzheimer's disease and epilepsy.
Shic
Frederick
F
Rudi Schulte Research Institute, Santa Barbara, CA, USA.
Ross
Brian
B
D001921:Brain / Q000378:metabolism*; D002247:Carbon Isotopes; D001330:Electronic Data Processing; D004827:Epilepsy / Q000378:metabolism; D005947:Glucose / Q000008:administration & dosage / Q000378:metabolism*; D018698:Glutamic Acid / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008432:Mathematical Computing; D015588:Observer Variation
D002247:Carbon Isotopes; D005973:Glutamine; D018698:Glutamic Acid; D005947:Glucose