Lethal photosensitization in microbiological treatment of ligature-induced peri-implantitis: a preliminary study in dogs.
This pilot study evaluated, by culture testing, the effectiveness of lethal photosensitization for the microbiological treatment of peri-implantitis in dogs. Experimental peri-implantitis was induced by ligature placement for 2 months. Following ligature removal, plaque control was instituted by scrubbing with 0.12% chlorhexidine daily for 12 months. Subsequently, mucoperiosteal flaps were elevated for scaling the implant surface. Microbial samples were obtained with paper points before and after treatment of implant surfaces by means of 100 microg/ml toluidine blue O (TBO,) and were exposed, for 80 s, to light with a wavelength of 685 nm from a 50 mW GaAlAs diode laser. The mean initial and final bacterial counts were 7.22 +/- 0.20 and 6.84 +/- 0.44 CFU/ml, respectively for TVC (P < 0.0001); 6.19 +/- 0.45 and 3.14 +/- 3.29 CFU/ml for P. intermedia/nigrescens (P = 0.001); 5.98 +/- 0.38 and 1.69 +/- 2.90 CFU/ml for Fusobacterium spp. (P = 0.001); and 6.07 +/- 0.22 to 1.69 +/- 2.94 CFU/ml for beta-hemolytic Streptococcus (P = 0.0039). It may be concluded that lethal photosensitization resulted in a reduction of the bacterial count. Complete elimination of bacteria was achieved in some samples.
Shibli
Jamil Awad
JA
Department of Periodontology, Dental School at Araraquara, State University of Säo Paulo (UNESP), Araraquara, SP, Brazil.
Martins
Marilia Compagnoni
MC
Theodoro
Letícia Helena
LH
Lotufo
Roberto Fraga Moreira
RF
Garcia
Valdir Gouveia
VG
Marcantonio
Elcio Júnior
EJ
D000818:Animals; D001421:Bacteria, Anaerobic / Q000187:drug effects*; D015169:Colony Count, Microbial; D015921:Dental Implants / Q000009:adverse effects / Q000382:microbiology*; D004285:Dogs; D005673:Fusobacterium / Q000187:drug effects; D053685:Laser Therapy; D008026:Ligation; D010518:Periodontitis / Q000188:drug therapy* / Q000209:etiology / Q000382:microbiology*; D017319:Photosensitizing Agents / Q000494:pharmacology / Q000627:therapeutic use*; D010865:Pilot Projects; D016966:Porphyromonas gingivalis / Q000187:drug effects; D018713:Prevotella intermedia / Q000187:drug effects; D018709:Statistics, Nonparametric; D013291:Streptococcus / Q000187:drug effects; D014048:Tolonium Chloride / Q000494:pharmacology / Q000627:therapeutic use
D015921:Dental Implants; D017319:Photosensitizing Agents; D014048:Tolonium Chloride