Chitosan nanoparticles containing plasmid DNA encoding house dust mite allergen, Der p 1 for oral vaccination in mice.
Our previous studies indicated that intramuscular (i.m.) immunisation with full length Der p 1 cDNA induced significant humoral response to the left domain (approximately corresponding to amino acids 1-116) but not to the right domain (approximately corresponding to amino acids 117-222) of Der p 1 allergen. This study explored the use of chitosan-DNA nanoparticles for oral immunisation to induce immune responses specific to both the left and right domains of Der p 1. DNA constructs pDer p 1 (1-222) and pDer p 1 (114-222) were complexed with chitosan and delivered orally followed by an i.m. injection of pDer p 1 (1-222) 13 weeks later. Such approach has successfully primed Th1-skewed immune responses against both domains of Der p 1. This strategy could be further optimised for more efficacious gene vaccination for full length Der p 1.
Chew
Joon Lin
JL
Department of Paediatrics, Faculty of Medicine, National University of Singapore, Lower Kent Ridge Road, Singapore 119074, Singapore.
Wolfowicz
Claudia Betina
CB
Mao
Hai-Quan
HQ
Leong
Kam W
KW
Chua
Kaw Yan
KY
D000284:Administration, Oral; D000485:Allergens / Q000235:genetics* / Q000276:immunology; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000235:genetics* / Q000276:immunology; D060829:Arthropod Proteins; D002686:Chitin / Q000031:analogs & derivatives*; D048271:Chitosan; D003546:Cysteine Endopeptidases; D004247:DNA / Q000008:administration & dosage* / Q000276:immunology; D004337:Drug Carriers; D005260:Female; D007074:Immunoglobulin G / Q000096:biosynthesis; D007273:Injections, Intramuscular; D051379:Mice; D008807:Mice, Inbred BALB C; D036103:Nanotechnology; D010316:Particle Size; D010957:Plasmids; D039981:Pyroglyphidae / Q000276:immunology*; D013997:Time Factors; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology
D000485:Allergens; D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; D004337:Drug Carriers; D007074:Immunoglobulin G; D019444:Vaccines, DNA; D002686:Chitin; D004247:DNA; D048271:Chitosan; D003546:Cysteine Endopeptidases; C451162:Dermatophagoides pteronyssinus antigen p 1