Analysis of natural allelic variation at seed dormancy loci of Arabidopsis thaliana.
Arabidopsis accessions differ largely in their seed dormancy behavior. To understand the genetic basis of this intraspecific variation we analyzed two accessions: the laboratory strain Landsberg erecta (Ler) with low dormancy and the strong-dormancy accession Cape Verde Islands (Cvi). We used a quantitative trait loci (QTL) mapping approach to identify loci affecting the after-ripening requirement measured as the number of days of seed dry storage required to reach 50% germination. Thus, seven QTL were identified and named delay of germination (DOG) 1-7. To confirm and characterize these loci, we developed 12 near-isogenic lines carrying single and double Cvi introgression fragments in a Ler genetic background. The analysis of these lines for germination in water confirmed four QTL (DOG1, DOG2, DOG3, and DOG6) as showing large additive effects in Ler background. In addition, it was found that DOG1 and DOG3 genetically interact, the strong dormancy determined by DOG1-Cvi alleles depending on DOG3-Ler alleles. These genotypes were further characterized for seed dormancy/germination behavior in five other test conditions, including seed coat removal, gibberellins, and an abscisic acid biosynthesis inhibitor. The role of the Ler/Cvi allelic variation in affecting dormancy is discussed in the context of current knowledge of Arabidopsis germination.
Alonso-Blanco
Carlos
C
Laboratory of Genetics, Graduate School of Experimental Plant Science, Wageningen University, The Netherlands.
Bentsink
Leónie
L
Hanhart
Corrie J
CJ
Blankestijn-de Vries
Hetty
H
Koornneef
Maarten
M
D000040:Abscisic Acid / Q000037:antagonists & inhibitors; D000483:Alleles; D017360:Arabidopsis / Q000235:genetics*; D002455:Cell Division; D002874:Chromosome Mapping; D005819:Genetic Markers; D014644:Genetic Variation; D005838:Genotype; D018525:Germination; D005875:Gibberellins / Q000494:pharmacology; D008957:Models, Genetic; D040641:Quantitative Trait Loci; D012639:Seeds / Q000502:physiology*; D013045:Species Specificity; D013696:Temperature; D013997:Time Factors
D005819:Genetic Markers; D005875:Gibberellins; D000040:Abscisic Acid