Cloning of a pore-forming subunit of ATP-sensitive potassium channel from Clonorchis sinensis.
A complete cDNA sequence encoding a pore-forming subunit (Kir6.2) of ATP-sensitive potassium channel in the adult worm, Clonorchis sinensis, termed CsKir6.2, was isolated from an adult cDNA library. The cDNA contained a single open-reading frame of 333 amino acids, which has a structural motif (a GFG-motif) of the putative pore-forming loop of the Kir6.2. Peculiarly, the CsKir6.2 shows a lack-sequence structure, which deleted 57 amino acids were deleted from its N-terminus. The predicted amino acid sequence revealed a highly conserved sequence as other known other Kir6.2 subunits. The mRNA was weekly expressed in the adult worm.
Hwang
Seung-Young
SY
Department of Parasitology and Institute of Malariology, Inje University College of Medicine, Busan 614-735, Korea.
Han
Hye-Jin
HJ
Kim
So-Hee
SH
Park
Sae-Gwang
SG
Seog
Dae-Hyun
DH
Kim
Na Ri
NR
Han
Jin
J
Chung
Joon-Yong
JY
Kho
Weon-Gyu
WG
D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003004:Clonorchis sinensis / Q000235:genetics* / Q000378:metabolism; D015801:Helminth Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D024661:Potassium Channels, Inwardly Rectifying / Q000235:genetics* / Q000378:metabolism; D018510:RNA, Helminth / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment
D015801:Helminth Proteins; D024661:Potassium Channels, Inwardly Rectifying; D018510:RNA, Helminth; D000255:Adenosine Triphosphate