Reverse micellar extraction and precipitation of lysozyme using sodium di(2-ethylhexyl) sulfosuccinate.
Sodium di(2-ethylhexyl) sulfosuccinate, referred to as Aerosol-OT or AOT, was used to remove lysozyme from an aqueous phase via reverse micellar extraction and precipitation method. For both methods, when the surfactant was in excess, a complete removal of lysozyme from the aqueous phase was obtained at the values of pH below the pI of lysozyme. However, for the reverse micellar method, a solubilization limit of lysozyme in the organic phase was observed, and a white precipitate was formed at the aqueous-organic interface. This observation suggested using AOT directly as a precipitating ligand. The lysozyme precipitated with AOT was fully recovered, with its original enzymatic activity, using acetone as a recovery solvent. A mechanism is suggested to explain the solubilization of lysozyme in an AOT reverse micellar system. It is shown that a direct precipitation method can be used with advantage instead of using the reverse micellar extraction method to recover lysozyme from an aqueous phase.
Shin
Youn-Ok
YO
Department of Chemical Engineering, McGill University, 3610 University Street, Montreal, Quebec, Canada H3A 2B2.
Weber
Martin E
ME
Vera
Juan H
JH
D011232:Chemical Precipitation; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003198:Computer Simulation; D004143:Dioctyl Sulfosuccinic Acid; D004563:Electrochemistry / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical; D009113:Muramidase / Q000737:chemistry* / Q000302:isolation & purification*; D012995:Solubility; D012996:Solutions; D055672:Static Electricity; D013386:Succinates / Q000737:chemistry*
D012996:Solutions; D013386:Succinates; D004143:Dioctyl Sulfosuccinic Acid; D009113:Muramidase