Kinetics of ingested host immunoglobulin G in hemolymph and whole body homogenates during nymphal development of Dermacentor variabilis and Ixodes scapularis ticks (Acari: Ixodidae).
Patterns in the utilization of host immunoglobulin G (IgG) during nymphal development differed between Dermacentor varibilis (Say) and Ixodes scapularis Say ticks. In unfed nymphs of D. variabilis, host IgG was readily detectable in both hemolymph and whole body homogenates. In unfed nymphs of I. scapularis, host IgG was absent in hemolymph and at very low concentrations in whole body homogenates. Host IgG in unfed nymphs was undoubtedly the remnants of IgG acquired during the larval bloodmeal that persisted through metamorphosis to the nymphal stage. In both tick species, host IgG crossed the midgut into the hemocoel during the latter phases of engorgement. Concentrations of host IgG in I. scapularis declined considerably after replete nymphs molted to the adult stage. In contrast, concentrations of host IgG in D. variabilis remained elevated throughout metamorphosis to the adult stage. When larval D. variabilis were fed on a rat, then 2 months later as nymphs on a rabbit, the rat IgG ("old IgG") present in unfed nymphs was totally replaced by rabbit IgG ("new IgG") within 2 d of nymphs attaching to the rabbit. Presumably, the old IgG acquired from a previous bloodmeal was secreted via saliva into the new host.
Vaughan
Jefferson A
JA
Department of Biology, University of North Dakota, Grand Forks, North Dakota 58201, USA. jefferson_vaughan@und.nodak.edu
Sonenshine
Daniel E
DE
Azad
Abdu F
AF
D000818:Animals; D006458:Hemolymph / Q000276:immunology / Q000378:metabolism*; D006790:Host-Parasite Interactions; D007074:Immunoglobulin G / Q000008:administration & dosage / Q000276:immunology / Q000378:metabolism*; D026863:Ixodidae / Q000254:growth & development* / Q000276:immunology / Q000378:metabolism*; D007700:Kinetics; D009758:Nymph / Q000254:growth & development* / Q000276:immunology / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D013045:Species Specificity
D007074:Immunoglobulin G