title: Oligomerization of adenosine A2A and dopamine D2 receptors in living cells.
abstract: We investigated whether oligomerization of adenosine A(2A) receptor (A(2A)R) and dopamine D(2) receptor (D(2)R) exists in living cells using modified bioluminescence resonance energy transfer (BRET(2)) technology. Fusion of these receptors to a donor, Renilla luciferase (Rluc), and to an acceptor, modified green fluorescent protein (GFP(2)), did not affect the ligand binding affinity, subcellular distribution, and coimmunoprecipitation of the receptors. BRET was detected not only between Myc-D(2)R-Rluc and A(2A)R-GFP(2) but also between HA-tagged A(2A)R-Rluc and A(2A)R-GFP(2). These results indicate A(2A)R, either homomeric or heteromeric with D(2)R, exists as an oligomer in living cells.
authors.affiliation: Department of Molecular Cell Signaling, Tokyo Metropolitan Institute for Neuroscience, 2-6 Musashidai, Fuchu, 183-8526, Tokyo, Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Kamiya
authors.forename: Osamu
authors.initials: O
authors.lastname: Saitoh
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Yoshioka
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Nakata
mesh_terms: D002460:Cell Line; D019281:Dimerization; D004735:Energy Transfer; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D006801:Humans; D007700:Kinetics; D008024:Ligands; D008164:Luminescent Proteins / Q000378:metabolism; D008856:Microscopy, Fluorescence; D011233:Precipitin Tests; D011485:Protein Binding; D043705:Receptor, Adenosine A2A; D017448:Receptors, Dopamine D2 / Q000737:chemistry*; D018047:Receptors, Purinergic P1 / Q000737:chemistry*; D013053:Spectrophotometry; D014162:Transfection
chemical_list: D008024:Ligands; D008164:Luminescent Proteins; D043705:Receptor, Adenosine A2A; D017448:Receptors, Dopamine D2; D018047:Receptors, Purinergic P1; D049452:Green Fluorescent Proteins