Sulfurihydrogenibium subterraneum gen. nov., sp. nov., from a subsurface hot aquifer.
A polyphasic taxonomic study was performed on a novel facultatively anaerobic, hydrogen- or sulfur/thiosulfate-oxidizing, thermophilic chemolithoautotroph recently isolated from subsurface hot aquifer water in a Japanese gold mine. The cells were straight to slightly curved rods, with a single polar flagellum. Growth was observed at 40-70 degrees C (optimum 60-65 degrees C; 80 min doubling time) and at pH 6.4-8.8 (optimum pH 7.5). The isolate was unable to use complex organic compounds, carbohydrates, amino acids or organic acids as sole energy and carbon sources. The G + C content of the genomic DNA was 31.3 mol%. Phylogenetic analysis based on 16S rDNA sequences indicated that the isolate was closely related to an uncultivated group of micro-organisms within the order Aquificales obtained from Icelandic and Japanese hot spring microbial mats, but distantly related to previously identified genera of the Aquificales such as Persephonella, Aquifex and Hydrogenobacter. The name Sulfurihydrogenibium subterraneum gen. nov., sp. nov. is proposed for this novel species; the type strain is HGMK1(T) (= JCM 11477(T) = ATCC BAA-562(T) = DSM 15120(T)).
Takai
Ken
K
Subground Animalcule Retrieval (SUGAR) Project, Frontier Research System for Extremophiles, Japan Marine Science & Technology Center, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan.
Kobayashi
Hideki
H
The DEEPSTAR Group, Frontier Research System for Extremophiles, Japan Marine Science & Technology Center, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan.
Nealson
Kenneth H
KH
NASA Jet Propulsion Laboratory, 4800 Oak Grove Drive, Pasadena, CA 91109-8099, USA.|Department of Earth Sciences, University of Southern California, Los Angeles, CA 90089-0740, USA.|Subground Animalcule Retrieval (SUGAR) Project, Frontier Research System for Extremophiles, Japan Marine Science & Technology Center, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan.
Horikoshi
Koki
K
The DEEPSTAR Group, Frontier Research System for Extremophiles, Japan Marine Science & Technology Center, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan.|Subground Animalcule Retrieval (SUGAR) Project, Frontier Research System for Extremophiles, Japan Marine Science & Technology Center, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan.
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004275:DNA, Ribosomal; D006046:Gold; D006089:Gram-Negative Anaerobic Bacteria / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D006358:Hot Temperature; D006859:Hydrogen / Q000378:metabolism; D007564:Japan; D008906:Mining; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S; D017422:Sequence Analysis, DNA; D013455:Sulfur / Q000378:metabolism; D013885:Thiosulfates / Q000378:metabolism; D014871:Water Microbiology
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D013885:Thiosulfates; D013455:Sulfur; D006046:Gold; D006859:Hydrogen