title: Contribution of nitric oxide to the depression of neuronal activity induced by temperature increase in the rat hippocampal CA1 area.
abstract: Using optical recording techniques, we examined whether nitric oxide (NO) is implicated in the impairment of the activity of hippocampal CA1 neurons induced by mild heat stress. A temperature increase from 32 to 38 degrees C reversibly depressed the neuronal activity in hippocampal slices. L-Arginine (1 mM), an NO donor, enhanced the heat-induced depression of the activity of hippocampal CA1 neurons. N(omega)-Nitro-L-arginine methyl ester, an inhibitor of nitric oxide synthase, attenuated the inhibition of the neuronal activity induced by a temperature increase. Methylcobalamin (10 microM), a vitamin B(12) analogue that reduces NO production, reduced the heat-induced depression of the neuronal activity. These results suggest that NO contributes, at least in part, to the heat-induced depression of the neuronal activity in the hippocampal CA1 region.
authors.affiliation: Department of Physiology, Kurume University School of Medicine, 67 Asahi-machi, 830-0011, Kurume, Japan.
authors.forename: Mitsue
authors.initials: M
authors.lastname: Takeya
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hasuo
authors.forename: Takashi
authors.initials: T
authors.lastname: Akasu
mesh_terms: D000818:Animals; D001120:Arginine / Q000494:pharmacology; D004558:Electric Stimulation; D004594:Electrophysiology; D006361:Heating; D006624:Hippocampus / Q000166:cytology / Q000502:physiology*; D066298:In Vitro Techniques; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009474:Neurons / Q000502:physiology*; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000502:physiology*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D055095:Optics and Photonics; D051381:Rats; D017208:Rats, Wistar; D013696:Temperature; D014805:Vitamin B 12 / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D020030:Nitric Oxide Donors; D009569:Nitric Oxide; D001120:Arginine; C019476:mecobalamin; D019001:Nitric Oxide Synthase; D014805:Vitamin B 12; D019331:NG-Nitroarginine Methyl Ester