Spatial distribution analysis of AT- and GC-rich regions in nuclei using corrected fluorescence resonance energy transfer.
We employed microscopic intensity-based fluorescence resonance energy transfer (FRET) images with correction by donor and acceptor concentrations to obtain unbiased maps of spatial distribution of the AT- and GC-rich DNA regions in nuclei. FRET images of 137 bovine aortic endothelial cells stained by the AT-specific donor Hoechst 33258 and the GC-specific acceptor 7-aminoactinomycin D were acquired and corrected for the donor and acceptor concentrations by the Gordon's method based on the three fluorescence filter sets. The corrected FRET images were quantitatively analyzed by texture analysis to correlate the spatial distribution of the AT- and GC-rich DNA regions with different phases of the cell cycle. Both visual observation and quantitative texture analysis revealed an increased number and size of the low FRET efficiency centers for cells in the G(2)/M-phases, compared to the G(1)-phase cells. We have detected cell cycle-dependent changes of the spatial organization and separation of the AT- and GC-rich DNA regions. Using the corrected FRET (cFRET) technique, we were able to detect early DNA separation stages in late interphase nuclei.
Murata
Shin-Ichi
S
Department of Pathology, University of Yamanashi School of Medicine, 1110 Shimokato, Tamaho-cho Nakakoma-gun, Yamanishi, Japan 409-3898. smurata@res.yamanashi-med.ac.jp
Herman
Petr
P
Mochizuki
Kunio
K
Nakazawa
Tadao
T
Kondo
Tetsuo
T
Nakamura
Nobuki
N
Lakowicz
Joseph R
JR
Katoh
Ryohei
R
D020856:AT Rich Sequence; D000818:Animals; D001011:Aorta / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D002417:Cattle; D002453:Cell Cycle; D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D031541:Fluorescence Resonance Energy Transfer; D020862:GC Rich Sequence
D004247:DNA