Borrelidin induces the transcription of amino acid biosynthetic enzymes via a GCN4-dependent pathway.
Global cellular profiling of messenger RNA levels has been used to provide insight into the effects of the angiogenesis inhibitor borrelidin on the eukaryotic model organism Saccharomyces cerevisiae. The most notable result of treatment with borrelidin is the induction of amino acid biosynthetic enzymes in a time-dependent fashion. We have ascertained that induction of this pathway involves the GCN4 transcription factor. This conclusion was determined by treating a yeast strain lacking this gene and observing the absence of increased gene transcription under Gcn4p control.
Eastwood
Erin L
EL
Department of Chemistry, Metcalf Center for Science and Engineering, Boston University, 590 Commonwealth Avenue, MA 02215, Boston, USA.
Schaus
Scott E
SE
D000596:Amino Acids / Q000096:biosynthesis*; D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D004268:DNA-Binding Proteins / Q000235:genetics*; D004798:Enzymes / Q000096:biosynthesis*; D005233:Fatty Alcohols / Q000494:pharmacology*; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D011494:Protein Kinases / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*
D000596:Amino Acids; D020533:Angiogenesis Inhibitors; D004268:DNA-Binding Proteins; D004798:Enzymes; D005233:Fatty Alcohols; D029701:Saccharomyces cerevisiae Proteins; C005066:borrelidin; D011494:Protein Kinases