title: Structure of the O-polysaccharide of Providencia alcalifaciens O21 containing 3-formamido-3,6-dideoxy-D-galactose.
abstract: The O-polysaccharide (O-antigen) of Providencia alcalifaciens O21 was obtained by mild acid degradation of the lipopolysaccharide and studied by chemical methods and NMR spectroscopy. It was found that the polysaccharide is built up of branched pentasaccharide repeating units with a terminal residue of 3-formamido-3,6-dideoxy-D-galactose (D-Fuc3NFo) and has the following structure: [structure: see text]. Anti-P. alcalifaciens O21 serum cross-reacted with the O-antigen of Proteus vulgaris O47, which contains a GalNAc trisaccharide similar to that present in the P. alcalifaciens O21 O-polysaccharide.
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 119991, GSP-1, Moscow, Russia.
authors.forename: Nina A
authors.initials: NA
authors.lastname: Kocharova
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Maszewska
authors.forename: George V
authors.initials: GV
authors.lastname: Zatonsky
authors.forename: Olga V
authors.initials: OV
authors.lastname: Bystrova
authors.forename: Andrzej
authors.initials: A
authors.lastname: Ziolkowski
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Torzewska
authors.forename: Aleksander S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
authors.forename: Antoni
authors.initials: A
authors.lastname: Rozalski
mesh_terms: D000116:Acetylgalactosamine / Q000032:analysis; D000606:Amino Sugars / Q000032:analysis; D002240:Carbohydrate Sequence; D003429:Cross Reactions / Q000276:immunology; D006603:Hexuronic Acids / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D025942:Oligosaccharides, Branched-Chain / Q000737:chemistry* / Q000276:immunology; D011514:Proteus vulgaris / Q000737:chemistry / Q000276:immunology; D011532:Providencia / Q000737:chemistry*
chemical_list: C108162:3-amino-3,6-dideoxygalactose; D000606:Amino Sugars; D006603:Hexuronic Acids; D019081:O Antigens; D025942:Oligosaccharides, Branched-Chain; C007819:galacturonic acid; D000116:Acetylgalactosamine