title: Allofustis seminis gen. nov., sp. nov., a novel Gram-positive, catalase-negative, rod-shaped bacterium from pig semen.
abstract: An unknown Gram-positive, catalase-negative, facultatively anaerobic, non-spore-forming, rod-shaped bacterium originating from semen of a pig was characterized using phenotypic, molecular chemical and molecular phylogenetic methods. Chemical studies revealed the presence of a directly cross-linked cell wall murein based on L-lysine and a DNA G + C content of 39 mol%. Comparative 16S rRNA gene sequencing showed that the unidentified rod-shaped organism formed a hitherto unknown subline related, albeit loosely, to Alkalibacterium olivapovliticus, Alloiococcus otitis, Dolosigranulum pigrum and related organisms, in the low-G + C-content Gram-positive bacteria. However, sequence divergence values of > 11% from these recognized taxa clearly indicated that the novel bacterium represents a separate genus. Based on phenotypic and phylogenetic considerations, it is proposed that the unknown bacterium from pig semen be classified as a new genus and species, Allofustis seminis gen. nov., sp. nov. The type strain is strain 01-570-1(T) (= CCUG 45438(T) = CIP 107425(T)).
authors.affiliation: School of Food Biosciences, University of Reading, Whiteknights, Reading RG6 6AP, UK.
authors.forename: Matthew D
authors.initials: MD
authors.lastname: Collins
authors.affiliation: Université de Montréal, Faculté de Médecine Vétérinaire, St Hyacinthe, Quebec, Canada.
authors.forename: Robert
authors.initials: R
authors.lastname: Higgins
authors.affiliation: Université de Montréal, Faculté de Médecine Vétérinaire, St Hyacinthe, Quebec, Canada.
authors.forename: Serge
authors.initials: S
authors.lastname: Messier
authors.affiliation: Université de Montréal, Faculté de Médecine Vétérinaire, St Hyacinthe, Quebec, Canada.
authors.forename: Madeleine
authors.initials: M
authors.lastname: Fortin
authors.affiliation: School of Food Biosciences, University of Reading, Whiteknights, Reading RG6 6AP, UK.
authors.forename: Roger A
authors.initials: RA
authors.lastname: Hutson
authors.affiliation: School of Food Biosciences, University of Reading, Whiteknights, Reading RG6 6AP, UK.
authors.forename: Paul A
authors.initials: PA
authors.lastname: Lawson
authors.affiliation: Culture Collection, Department of Clinical Bacteriology, University of Göteborg, Sweden.
authors.forename: Enevold
authors.initials: E
authors.lastname: Falsen
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002374:Catalase / Q000378:metabolism; D002473:Cell Wall / Q000737:chemistry; D004275:DNA, Ribosomal / Q000032:analysis; D016987:Gram-Positive Rods / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D008297:Male; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000032:analysis; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012661:Semen / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013552:Swine
chemical_list: D004275:DNA, Ribosomal; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D002374:Catalase