Purification of peroxisomes in a preformed iodixanol gradient in a fixed-angle rotor.
In iodixanol, peroxisomes are the densest organelle in the light mitochondrial fraction and are therefore easily separated from the other components (lysosomes, mitochondria, etc.) in a preformed isosmotic continuous gradient. The resolution of the peroxisomes is far superior than that in sucrose and, unlike in Percoll there is no contamination from endoplasmic reticulum.
Graham
John M
JM
School of Biomolecular Sciences, Liverpool John Moores University. john@jgrescon.fsbusiness.co.uk
D000818:Animals; D002458:Cell Fractionation / Q000295:instrumentation / Q000379:methods*; D002499:Centrifugation, Density Gradient / Q000295:instrumentation / Q000379:methods*; D008099:Liver / Q000648:ultrastructure; D020675:Peroxisomes; D051381:Rats; D014283:Triiodobenzoic Acids / Q000737:chemistry*
D014283:Triiodobenzoic Acids; C044834:iodixanol