Actinobaculum urinale sp. nov., from human urine.
A hitherto undescribed Actinomyces-like bacterium was isolated from human urine. Based on its biochemical characteristics, the unidentified bacterium did not correspond to any currently described Actinomyces species or related taxa. Comparative 16S rRNA gene sequencing showed that the unknown bacterium exhibits a specific phylogenetic association with the genus Actinobaculum, but a sequence divergence of > 5% from the two currently recognized members of this genus, Actinobaculum schaalii and Actinobaculum suis, demonstrates that it represents a distinct species. Based on both phenotypic and 16S rRNA gene sequence considerations, it is proposed that the unknown bacterium from urine should be classified as a novel species, Actinobaculum urinale sp. nov. The type strain of Actinobaculum urinale is CCUG 46093(T) (= CIP 107424(T)).
Hall
Val
V
Anaerobe Reference Unit, PHLS, University Hospital of Wales, Cardiff CF14 4XW, UK.
Collins
Matthew D
MD
School of Food Biosciences, University of Reading, Reading, UK.
Hutson
Roger A
RA
School of Food Biosciences, University of Reading, Reading, UK.
Falsen
Enevold
E
Culture Collection, Department of Clinical Bacteriology, University of Göteborg, Göteborg, Sweden.
Inganäs
Elisabeth
E
Culture Collection, Department of Clinical Bacteriology, University of Göteborg, Göteborg, Sweden.
Duerden
Brian I
BI
Anaerobe Reference Unit, PHLS, University Hospital of Wales, Cardiff CF14 4XW, UK.
D000191:Actinomycetaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D000193:Actinomycetales Infections / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D005260:Female; D020459:Genes, rRNA; D006801:Humans; D008969:Molecular Sequence Data; D010641:Phenotype; D011776:Pyuria / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011933:Reagent Kits, Diagnostic; D017422:Sequence Analysis, DNA; D014556:Urine / Q000382:microbiology*
D012336:RNA, Ribosomal, 16S; D011933:Reagent Kits, Diagnostic