title: Spleen transplantation in miniature swine: surgical technique and results in major histocompatibility complex-matched donor and recipient pairs.
abstract: BACKGROUND
  Spleen transplantation (Tx) between some strains of rodents can lead to donor-specific tolerance either spontaneously or after a short course of immunosuppression. This study developed a surgical technique for spleen Tx in miniature swine to investigate its immunologic impact in a large animal model.
  
  
  METHODS
  The preferred surgical technique of spleen Tx (n=8) involved excision of the donor spleen with its vascular pedicle to the aorta and portal vein. Carrel patches of donor aorta and portal vein were anastomosed to the abdominal aorta and inferior vena cava, respectively, of the (splenectomized) recipient. The results in four major histocompatibility complex-matched pairs that were mismatched for the porcine allelic antigen are reported. Two recipients were untreated, one received a 12-day course of cyclosporine A (CsA) alone, and one received thymic irradiation (700 cGy) and CsA. Hematopoietic cell chimerism was followed by fluorescence-activated cell sorter, and graft survival was assessed by histology.
  
  
  RESULTS
  Spleen Tx was technically successful. In two untreated pigs, chimerism was detected in the blood (maximum 5% for 17 and 25 days) and lymph nodes (maximum 6% for 28 and 56 days), but both grafts showed histologic rejection by day 28. In two treated pigs, chimerism was present in the blood for 47 and 57 days, and rejection was prevented, with follow-up for 57 and 217 days, respectively.
  
  
  CONCLUSIONS
  Spleen Tx in major histocompatibility complex-matched pairs treated with CsA+/-thymic irradiation results in prolonged chimerism and is associated with the development of in vivo unresponsiveness to the transplanted spleen.
authors.affiliation: Transplantation Biology Research Center, Massachusetts General Hospital/Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: B
authors.initials: B
authors.lastname: Gollackner
authors.forename: F J M F
authors.initials: FJ
authors.lastname: Dor
authors.forename: C
authors.initials: C
authors.lastname: Knosalla
authors.forename: L
authors.initials: L
authors.lastname: Buhler
authors.forename: M
authors.initials: M
authors.lastname: Duggan
authors.forename: C A
authors.initials: CA
authors.lastname: Huang
authors.forename: S L
authors.initials: SL
authors.lastname: Houser
authors.forename: D H
authors.initials: DH
authors.lastname: Sachs
authors.forename: T
authors.initials: T
authors.lastname: Kawai
authors.forename: D S C
authors.initials: DS
authors.lastname: Ko
authors.forename: D K C
authors.initials: DK
authors.lastname: Cooper
mesh_terms: D000818:Animals; D001706:Biopsy; D016572:Cyclosporine / Q000494:pharmacology; D005434:Flow Cytometry; D006084:Graft Rejection / Q000401:mortality / Q000473:pathology; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006650:Histocompatibility Testing; D007166:Immunosuppressive Agents / Q000494:pharmacology; D008285:Major Histocompatibility Complex / Q000276:immunology*; D011183:Postoperative Complications; D016038:Skin Transplantation / Q000276:immunology; D013154:Spleen / Q000473:pathology / Q000637:transplantation*; D013156:Splenectomy / Q000379:methods*; D013556:Swine, Miniature; D014019:Tissue Donors; D018183:Transplantation Chimera
chemical_list: D007166:Immunosuppressive Agents; D016572:Cyclosporine