Biodegradation of cyclic amines by a Pseudomonas strain involves an amine mono-oxygenase.
Pseudomonas putida O1G3 catalyzes the degradation of pyrrolidine and piperidine. This strain can use these compounds as the sole source of carbon, nitrogen, and energy. When the cyclic amines were used as the growth substrates, the synthesis of a soluble heme amine mono-oxygenase was induced in this bacteria. This observation was confirmed by spectrophotometric analysis and specific inhibitor. This mono-oxygenase is a NADH-dependent enzyme and catalyzes the cleavage of the C-N bond of the pyrrolidine and piperidine ring by a mechanism similar to a N dealkylation. This reaction could be followed by ring cleavage to form gamma-aminobutyraldehyde oxidized to gamma-aminobutyrate. Further investigations to purify the heme-containing mono-oxygenase are in progress.
Trigui
M
M
Laboratoire de génie enzymatique et cellulaire, UMR 6022 CNRS, Université de technologie de Compiègne, B.P. 20529-60205 Compiègne, France.
Pulvin
S
S
Poupin
P
P
Thomas
D
D
D000588:Amines / Q000737:chemistry / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002457:Cell Extracts; D003470:Culture Media; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry; D006899:Mixed Function Oxygenases / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D010880:Piperidines / Q000378:metabolism; D011190:Potassium Cyanide; D016958:Pseudomonas putida / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D011759:Pyrrolidines / Q000378:metabolism; D012988:Soil Microbiology; D013053:Spectrophotometry / Q000379:methods; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D000588:Amines; D002457:Cell Extracts; D003470:Culture Media; D010880:Piperidines; D011759:Pyrrolidines; D005680:gamma-Aminobutyric Acid; C032727:piperidine; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; C032519:pyrrolidine; D011190:Potassium Cyanide