Functional association of immediate early gene c-fos with nuclear matrix.
Accumulation of c-fos gene locus DNA in the nuclear matrix of hepatocyte nuclei was observed during induction of c-fos with cycloheximide. No enhanced association with the nuclear matrix was detected for inactive immunoglobulin gene locus. The use of endogenous DNAses allows isolation of nuclear matrix preparations enriched with transcribing chromatin.
Borisova
N P
NP
Laboratory of Molecular Biology, Institute of Chemical Physics Problems, Russian Academy of Medical Sciences, Chernogolovka, Moscow region.
Kostyuk
G V
GV
Shevchenko
N A
NA
Boikov
P Ya
PY
Rudakova
E V
EV
Papina
R I
RI
Terent'ev
A A
AA
D000818:Animals; D002843:Chromatin / Q000235:genetics / Q000378:metabolism*; D003513:Cycloheximide / Q000378:metabolism; D005786:Gene Expression Regulation; D016762:Genes, fos; D022781:Hepatocytes / Q000166:cytology / Q000502:physiology; D007074:Immunoglobulin G / Q000235:genetics; D008297:Male; D015530:Nuclear Matrix / Q000378:metabolism*; D011500:Protein Synthesis Inhibitors / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D002843:Chromatin; D007074:Immunoglobulin G; D011500:Protein Synthesis Inhibitors; D003513:Cycloheximide