title: Oral administration of proteoglycan isolated from Phellinus linteus in the prevention and treatment of collagen-induced arthritis in mice.
abstract: To examine whether oral administration of proteoglycan derived from Phellinus linteus, which is known as the medicinal mushroom, can prevent or treat collagen-induced arthritis (CIA) in mice as experimental model of autoimmune disease. CIA was induced by intradermal injection of type II collagen (CII) emulsified with complete freund's adjuvant (CFA) into the base of the tail (on day 7) followed by a booster injection on day 21 into the footpad. To examine the ability of proteoglycan to effect the inhibition of CIA, doses of proteoglycan were orally administered on day 0 (pre-administration) or day 28 (post-administration) at two groups. The inhibition of CIA by oral administration of proteoglycan was associated with decrease in anti-CII IgG and IgG2a antibodies (Abs) as well as varying kinds of cytokines including IL-12, TNF-alpha, and IFN-gamma. The results showed that administration of proteoglycan was followed by decrease of CIA of the mice in pre- and post-administration groups. Our findings suggest that immunomodulating proteoglycan isolated from P. linteus may be crucially involved in the prevention and treatment of autoimmune joint inflammation such as rheumatoid arthritis, although no definite role of anti-CII Abs in the human disease has been established.
authors.affiliation: Department of Microbiology, College of Natural Sciences, Pusan National University, Pusan 609-735, South Korea.
authors.forename: Gi-Young
authors.initials: GY
authors.lastname: Kim
authors.forename: Seung-Hoon
authors.initials: SH
authors.lastname: Kim
authors.forename: Sue-Yun
authors.initials: SY
authors.lastname: Hwang
authors.forename: Ho-Youn
authors.initials: HY
authors.lastname: Kim
authors.forename: Yeong-Min
authors.initials: YM
authors.lastname: Park
authors.forename: Soon-Kew
authors.initials: SK
authors.lastname: Park
authors.forename: Min-Ki
authors.initials: MK
authors.lastname: Lee
authors.forename: Sang-Hwa
authors.initials: SH
authors.lastname: Lee
authors.forename: Tae-Ho
authors.initials: TH
authors.lastname: Lee
authors.forename: Jae-Dong
authors.initials: JD
authors.lastname: Lee
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000302:isolation & purification / Q000627:therapeutic use*; D000284:Administration, Oral; D000818:Animals; D001169:Arthritis, Experimental / Q000188:drug therapy* / Q000276:immunology / Q000517:prevention & control*; D001487:Basidiomycota / Q000737:chemistry*; D024043:Collagen Type II / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005620:Freund's Adjuvant / Q000276:immunology; D015870:Gene Expression / Q000187:drug effects; D007114:Immunization; D007074:Immunoglobulin G / Q000276:immunology; D007518:Isoantibodies / Q000096:biosynthesis / Q000276:immunology; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D011509:Proteoglycans / Q000008:administration & dosage / Q000302:isolation & purification / Q000627:therapeutic use*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000276:Adjuvants, Immunologic; D024043:Collagen Type II; D016207:Cytokines; D007074:Immunoglobulin G; D007518:Isoantibodies; D011509:Proteoglycans; D012333:RNA, Messenger; D005620:Freund's Adjuvant