Facile and rapid high-performance liquid chromatography method for simultaneous determination of allopurinol and oxypurinol in human serum.
OBJECTIVE
To develop a rapid and sensitive assay for the simultaneous determination of allopurinol and oxypurinol in serum.


METHODS
High-performance liquid chromatography (HPLC) with UV-detection. Sample preparation consists of protein precipitation by an addition of trichloracetic acid.


RESULTS
Percentage recovery and intra-assay coefficient of variation (CV%) for allopurinol were 97.4-101.3 and 0.66-5.13, respectively, in the concentration range 0.5-5.0 microg/mL. For oxypurinol, the percentage recovery and the intra-assay CV% were 93.2-98.1 and 0.88-5.62, respectively, in the concentration ranges 0.4-20 microg/mL. There was no interference of endogenous compounds in this assay.


CONCLUSIONS
This method is useful for routine therapeutic drug monitoring of allopurinol in a clinical setting.
Tada
H
H
Department of Pharmaceutical Science, Akita University Hospital, 1-1-1 Hondo, Akita 010-8543, Japan.
Fujisaki
A
A
Itoh
K
K
Suzuki
T
T
D000284:Administration, Oral; D000493:Allopurinol / Q000097:blood* / Q000493:pharmacokinetics; D019540:Area Under Curve; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006074:Gout Suppressants / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D010117:Oxypurinol / Q000097:blood* / Q000493:pharmacokinetics; D014970:Xanthines / Q000493:pharmacokinetics
D006074:Gout Suppressants; D014970:Xanthines; D000493:Allopurinol; D010117:Oxypurinol