Tetraploid myeloid cells in donors of peripheral blood stem cells treated with rhG-CSF.
Recombinant human granulocyte colony-stimulating factor (rhG-CSF) is frequently used to mobilize CD34+ cells in healthy donors and patient with malignant diseases prior to peripheral blood stem cell (PBSC) harvest. To analyze the effects of rhG-CSF on morphology and genotype of white blood cells, a novel multiparametric cell scanning system that combines morphologic, immune and genotypic analyses of the same cells was used. We report here that tetraploid myeloid cells are present in the peripheral blood of donors treated with rhG-CSF. The tetraploidy was detected in up to 0.6% of differentiated myeloid cells and all observed CD34+ cells were diploid. Thus, short treatment with rhG-CSF of PBSC donors induces numerfical chromosomal alterations in a small subset of mature myeloid cells.
Kaplinsky
C
C
Department of Pediatric Hemato-Oncology, The Chaim Sheba Medical Center, Tel-Hashomer, Israel.
Trakhtenbrot
L
L
Hardan
I
I
Reichart
M
M
Daniely
M
M
Toren
A
A
Amariglio
N
N
Rechavi
G
G
Izraeli
S
S
D016022:Case-Control Studies; D048429:Cell Size / Q000187:drug effects; D002869:Chromosome Aberrations / Q000139:chemically induced; D020732:Cytogenetic Analysis; D004341:Drug Evaluation; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage / Q000494:pharmacology*; D042381:Granulocyte Precursor Cells / Q000166:cytology / Q000187:drug effects; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D006801:Humans; D007937:Leukapheresis; D008223:Lymphoma / Q000628:therapy; D008297:Male; D022423:Myeloid Cells / Q000166:cytology / Q000187:drug effects*; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects; D036102:Peripheral Blood Stem Cell Transplantation / Q000379:methods; D011123:Polyploidy; D011994:Recombinant Proteins; D014019:Tissue Donors
D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor