title: Lentivirus vector can integrate in the genome and exist and replicate in the cell as an episome.
abstract: Lethally irradiated mice were reconstituted with few purified primitive hemopoietic stem cells containing sequences of a gene encoding green fluorescent protein. The gene was transferred using a lentivirus vector. The presence of the marker gene in splenocyte colonies derived from the bone marrow of reconstituted mice and in cells of other hemopoietic and non-hemopoietic organs was studied during the life. It was shown that the lentivirus vector can persist for a long time and replicate in hemopoietic cells as an episome.
authors.affiliation: Hematological Research Center, Russian Academy of Medical Sciences, Moscow.
authors.forename: M A
authors.initials: MA
authors.lastname: Ershler
authors.forename: N I
authors.initials: NI
authors.lastname: Drize
authors.forename: I N
authors.initials: IN
authors.lastname: Nifontova
authors.forename: A V
authors.initials: AV
authors.lastname: Terskikh
authors.forename: I L
authors.initials: IL
authors.lastname: Chertkov
mesh_terms: D000818:Animals; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D016678:Genome; D049452:Green Fluorescent Proteins; D006412:Hematopoietic Stem Cells / Q000378:metabolism; D016086:Lentivirus / Q000235:genetics* / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D010957:Plasmids / Q000235:genetics* / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000502:physiology; D033581:Stem Cell Transplantation; D016662:Virus Integration; D014779:Virus Replication
chemical_list: D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins