title: An effective sporicidal reagent against Bacillus subtilis spores.
abstract: We developed a reagent which showed significant sporicidal activity against Bacillus subtilis spores. This reagent was composed of ethylenediaminetetraacetic acid, disodium salt (EDTA-2Na), ferric chloride hexahydrate (FeCl3 x 6H2O) and ethanol (tentatively designated as the ethanol reagent). The ethanol reagent showed pH- and temperature-dependent sporicidal activity. At pH 0.3, its activity was almost the same as that of 0.05% sodium hypochlorite at 20 C and was higher at 37 C than at 20 C. The activity of the ethanol reagent was similar both with and without 10% serum. The ethanol reagent might be applicable for disinfecting Bacillus spores.
authors.affiliation: Niigata Quarantine Station, Ministry of Health, Labour and Welfare, 1-5-4, Ryugashima, Niigata, Niigata 950-0072, Japan. n-kida@forth.go.jp
authors.forename: Nori
authors.initials: N
authors.lastname: Kida
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Mochizuki
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Taguchi
mesh_terms: D001412:Bacillus subtilis / Q000187:drug effects* / Q000254:growth & development; D004202:Disinfectants / Q000737:chemistry / Q000494:pharmacology*; D004492:Edetic Acid / Q000737:chemistry / Q000494:pharmacology; D000431:Ethanol / Q000737:chemistry / Q000494:pharmacology; D005290:Ferric Compounds / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D012965:Sodium Chloride / Q000737:chemistry / Q000494:pharmacology; D012973:Sodium Hypochlorite / Q000494:pharmacology; D013171:Spores, Bacterial / Q000187:drug effects
chemical_list: D004202:Disinfectants; D005290:Ferric Compounds; D000431:Ethanol; D012965:Sodium Chloride; D004492:Edetic Acid; D012973:Sodium Hypochlorite