The promoter of the spinach PsaF gene for the subunit III of the photosystem I reaction center directs beta-glucuronidase gene expression in transgenic tobacco roots. Implication of the involvement of phospholipases and protein kinase C in PsaF gene expression.
PsaF is a nuclear encoded gene for the subunit III of photosystem I. It is located at the lumenal side of the thylakoid membrane and interacts with plastocyanin. Starting from a low-level expression in the cotyledons of etiolated seedlings the gene is upregulated by light. Light can be replaced by Ca2+ or phosphoinositides like phorbol myristate acetate, an analogue of diacylglycerol. We tested the effects of these components on PsaF promoter-driven gene expression in roots and found that the PsaF promoter includes a positive regulatory region [-220/-179] activated by cytokinin and a negative regulatory region [-687/-221] activated by abscisic acid. In addition, the promoter is activated by Ca2+, mastoparan and phorbol myristate acetate which suggests a role for phospholipases and protein kinase C in PsaF gene expression.
Wöstemeyer
Anke
A
Institut für Allgemeine Botanik und Pflanzenphysiologie, Friedrich-Schiller-Universität Jena, Dornburger Strasse 159, 07743 Jena, Germany.
Oelmüller
Ralf
R
D000040:Abscisic Acid / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D003583:Cytokinins / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005966:Glucuronidase / Q000235:genetics*; D036341:Intercellular Signaling Peptides and Proteins; D008027:Light; D008565:Membrane Proteins / Q000235:genetics*; D010455:Peptides; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics*; D045331:Photosystem I Protein Complex; D010940:Plant Proteins; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics*; D021122:Protein Subunits / Q000235:genetics; D015398:Signal Transduction; D018724:Spinacia oleracea / Q000235:genetics*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics*; D014862:Wasp Venoms / Q000494:pharmacology
D003583:Cytokinins; D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D010455:Peptides; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D010940:Plant Proteins; D021122:Protein Subunits; D014862:Wasp Venoms; C037500:mastoparan; D000040:Abscisic Acid; D005966:Glucuronidase; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium