Dang-Gui-Bu-Xai-Tang modulated the immunity of tumor bearing mice.
Dang-Gui-Bu-Xai-Tang (DGBXT), which includes Radix Angelicae Sinensis and Radix Astragali Membranaceus, is a traditional Chinese medicine used to modulate the lymphocyte activity of cancer patients after chemotherapy and radiotherapy. In the present study, we examined the cytotoxicity of DGBXT on transformed cells and the immunomodulating effects of DGBXT in a tumor-bearing murine model. DGBXT markedly inhibited the growth of the EJ-Ha-ras transformed LZEJ and LZEJ-C2 cells lines. Oral administration of DGBXT for three weeks significantly prevented the tumor development in mice that injected with LZEJ-C2 cells subcutaneously. Moreover, DGBXT effectively increased the population of cytotoxic T lymphocytes (CTLs) and NK cells, and down-regulated activated T helper cells (CD4+/CD25+) in spleen and tumor-draining lymph nodes (TDLN). Furthermore, DGBXT stimulated the production of tumor necrosis factor-alpha in in vitro cultured splenocytes. These results might explain the antitumor effects of DGBXT.
Hsieh
Chang-Chi
CC
Institute of Chinese Pharmaceutical Science, China Medical College, Taichung, Taiwan, ROC. cchsieh@mail.cmc.edu.tw
Lin
Wen-Chang
WC
Lee
Miau-Rong
MR
Hsu
Shih-Lan
SL
Liu
Hsiao-Sheng
HS
Kao
Shung-Te
ST
Hsieh
Ming-Tsuen
MT
D016475:3T3 Cells; D000276:Adjuvants, Immunologic / Q000627:therapeutic use*; D000818:Animals; D002461:Cell Line, Transformed; D002470:Cell Survival / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D007109:Immunity / Q000187:drug effects; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000188:drug therapy / Q000276:immunology / Q000517:prevention & control; D013154:Spleen / Q000276:immunology / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000276:immunology
D000276:Adjuvants, Immunologic; D004365:Drugs, Chinese Herbal; D014409:Tumor Necrosis Factor-alpha