High-affinity monoclonal antibodies for detection of the microbial metabolite, 2-methylisoborneol.
The production of 2-methylisoborneol (MIB) by certain fungi and algae can contribute musty off-flavors to foods and water supplies if uncontrolled. The goal of this research was to develop a nonsensory simple method for the detection of MIB. Anti-MIB monoclonal antibodies were produced by immunizing mice with borneol-conjugated protein and selecting positive clones with an MIB-protein conjugate. An indirect competitive immunoassay developed using this antibody had a detection limit of 0.6 microg L(-)(1) and an I(50) value of 5 microg L(-)(1). Detection was relatively specific for MIB and showed 20% cross-reactivity with borneol or isoborneol and 4-5% cross-reactivity with camphor. No cross-reactivity to geosmin was observed.
Plhak
Leslie C
LC
Department of Food Science, Louisiana Agricultural Experiment Station and Louisiana State University, Baton Rouge 70803, USA. lcplhak@facstaff.wisc.edu
Park
Eun Sung
ES
D000818:Animals; D000911:Antibodies, Monoclonal; D001892:Camphanes / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D001892:Camphanes; C005536:2-methylisoborneol; C022871:isoborneol