Studies on the interaction of the antibiotic moenomycin A with the enzyme penicillin-binding protein 1b.
The interaction of a moenomycin derivative with the enzyme penicillin binding protein 1b (PBP 1b) has been studied by means of STD NMR. The results obtained initiated the synthesis of a number of moenomycin derivatives modified in unit A including a moenomycin-ampicillin conjugate and determination of their antibiotic activities. A protocol is described that allows studying the interaction of moenomycin analogues with PBP 1b by fluorescence correlation spectroscopy.
Rühl
Thomas
T
Universität Leipzig, Fakultät für Chemie und Mineralogie, Johannisallee 29, D-04103 Leipzig, Germany.
Daghish
Mohammed
M
Buchynskyy
Andrij
A
Barche
Karen
K
Volke
Daniela
D
Stembera
Katherina
K
Kempin
Uwe
U
Knoll
Dietmar
D
Hennig
Lothar
L
Findeisen
Matthias
M
Oehme
Ramona
R
Giesa
Sabine
S
Ayala
Juan
J
Welzel
Peter
P
D000900:Anti-Bacterial Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors*; D001455:Bambermycins / Q000138:chemical synthesis / Q000494:pharmacology*; D002352:Carrier Proteins / Q000037:antagonists & inhibitors*; D004058:Diffusion; D006602:Hexosyltransferases / Q000037:antagonists & inhibitors*; D008826:Microbial Sensitivity Tests; D002267:Muramoylpentapeptide Carboxypeptidase / Q000037:antagonists & inhibitors*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D017830:Octoxynol; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases / Q000037:antagonists & inhibitors*; D011485:Protein Binding; D013050:Spectrometry, Fluorescence
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002352:Carrier Proteins; D046915:Penicillin-Binding Proteins; D001455:Bambermycins; C437243:moenomycin A; D017830:Octoxynol; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase