Apoptotic activities of C2-ceramide and C2-dihydroceramide homologues against HL-60 cells.
The apoptotic activities of non-natural ceramide homologues, C2-homo-ceramide, C2-homo-dihydroceramide, C2-bishomo-ceramide and C2-bishomo-dihydroceramide, were examined using human leukemia HL-60 cells. The apoptotic activity was in order of C2-ceramide>C2-homo-ceramide approximately C2-bishomo-ceramide and the activities of the L-erythro- and D-erythro-ceramide homologues were similar. The morphological features of the cells, DNA fragmentations, proteolytic processing of pro-caspase-3 and the cleavage of PARP as the result of treatments with these homologues indicated that cell death was induced by apoptosis.
Shikata
Keiji
K
Department of Applied and Bioapplied Chemistry, Graduate School of Engineering, Osaka City University, Sugimoto 3-3-138, Sumiyoshi-ku, Osaka 558-8585, Japan.
Niiro
Hayato
H
Azuma
Hideki
H
Ogino
Kenji
K
Tachibana
Taro
T
D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D020169:Caspases / Q000032:analysis; D002518:Ceramides / Q000138:chemical synthesis / Q000494:pharmacology*; D053938:DNA Fragmentation; D004305:Dose-Response Relationship, Drug; D018922:HL-60 Cells; D006801:Humans; D007700:Kinetics; D011064:Poly Adenosine Diphosphate Ribose / Q000378:metabolism; D013110:Sphingosine / Q000031:analogs & derivatives*; D013329:Structure-Activity Relationship
D002518:Ceramides; C064769:N-acetylsphingosine; C109343:dihydroceramide; D011064:Poly Adenosine Diphosphate Ribose; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases; D013110:Sphingosine