Splicing goes global.
Transcriptomics, the analysis of the complement of mRNAs transcribed from a cell's genome, currently focuses mainly on mature, processed mRNAs. However, posttranscriptional processing of primary transcripts can significantly affect both the quantity and the structure of the mature mRNAs and therefore of the protein products. Recently, the development of an intron-specific microarray has permitted a preliminary analysis of the splicing of all intron-containing transcripts in Saccharomyces cerevisiae. Here, we discuss the findings and what might be learned from this kind of approach.
Barrass
J David
JD
Wellcome Trust Centre for Cell Biology, University of Edinburgh, King's Buildings, Mayfield Road, EH9 3JR, Edinburgh, Scotland, UK.
Beggs
Jean D
JD
D005656:Fungal Proteins / Q000235:genetics; D007438:Introns; D009694:Nucleic Acid Precursors; D020411:Oligonucleotide Array Sequence Analysis; D012322:RNA Precursors; D012323:RNA Processing, Post-Transcriptional; D012326:RNA Splicing; D012333:RNA, Messenger; D012441:Saccharomyces cerevisiae; D014158:Transcription, Genetic
D005656:Fungal Proteins; D009694:Nucleic Acid Precursors; D012322:RNA Precursors; D012333:RNA, Messenger