[Inducing chromosome translocation and deletions by Chinese Spring-Agilops 2C disomic addition x Chinese Spring-Elytriga 5E disomic addition].
Crossing between two disomic addition lines, Chinese Spring-E. elongata and Chinese Sping with two gametocidal chromosomes 2C (from Ae. cylindric), was carried out to investigate the function of gametocidal chromosome. After scrutinizing the meiosis of pollen mother cells (PMCs) in F1 hybrids, several results were concluded: (1) In seven of the crossing combinations, the number of univalents exceeded the expected and some trivalents and tetravalents appeared also in MI; lagging, breakage and bridge of chromosomes were observed in anaphase and telophase; considerable micronuclei formed in telophase and tetrads. These were mainly caused by the gametocidal chromosome 2C. (2) Chromosomes 6E and 7E were more susceptible to the effect of the gametocidal chromosome 2C. (3) The gametocidal chromosome 2C functioned in prophase viz. the period of forming synaptonemal complex. Four F1 lines, 5-14, 5-37, 5-67 and 5-71, were identified to be T5ES 4AST5EL 2BS, T5EL 3AS, T5ES 5BS translocation respectively by using C-banding and genome in situ hybrydization(GISH) analysis. Deletion was detected in line 5-17 (short arm of chromosome 2A), 5-27(6B), 5-18(4B and 5B), 5-72(4A) and 5-4(4B) by C-banding analysis. The statistic data showed that gametocidal chromosome could induce translocation with a high frequency of 5% and reacted on group B more efficiently than on groups A and D since translocation involving chromosome 4A, 2B, 3A, 5B and deletion involving chromosome 6B, 5B, 4B, 4A 2A according to Endo's work.
Li
Ji-Lin
JL
Harbin Normal University, Harbin 150080, China.
Xu
Xiang-Ling
XL
Xu
Ping
P
Guo
Chang-Hong
CH
D002871:Chromosome Banding / Q000379:methods; D002872:Chromosome Deletion; D032461:Chromosomes, Plant / Q000235:genetics*; D003433:Crosses, Genetic; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008540:Meiosis / Q000235:genetics; D006109:Poaceae / Q000235:genetics*; D011058:Pollen / Q000235:genetics; D014178:Translocation, Genetic