title: Determination of efficiency of attachment of biotinylated antibodies to avidin-linked, aqueous-based microcapsules by flow cytometry.
abstract: We used flow cytometry to determine the percentage of aqueous-based microcapsules bearing antibodies specific for various antigen-presenting cells (APCs) within a given population of putative APC-specific microcapsules. Flow cytometry offers a high-throughput, rapid and simple method to analyze antibody binding to noncellular, nonspherical material.
authors.affiliation: University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
authors.forename: Andrea A
authors.initials: AA
authors.lastname: Chow
authors.forename: Charlotte A
authors.initials: CA
authors.lastname: Moser
authors.forename: Tully J
authors.initials: TJ
authors.lastname: Speaker
authors.forename: Paul A
authors.initials: PA
authors.lastname: Offit
mesh_terms: D000818:Animals; D000906:Antibodies / Q000378:metabolism*; D000938:Antigen-Presenting Cells / Q000378:metabolism*; D001360:Avidin / Q000378:metabolism*; D001710:Biotin / Q000378:metabolism; D002214:Capsules; D005434:Flow Cytometry / Q000379:methods*
chemical_list: D000906:Antibodies; D002214:Capsules; D001360:Avidin; D001710:Biotin