[Research on the gap junctional intercellular communication of CHL cells and human cancer cells induced by c9, t11-conjugated linoleic acid].
In order to determine the effects on the gap junctional intercellular communication(GJIC) of CHL cells and human cancer cells induced by c9, t11-CLA and its mechanism. GJIC was observed by using scrape loading and dye transfer(SLDT) on CHL cells blocked by TPA and cancer cells(SGC-7901 and MCF-7) which were treated with various c9, t11-conjugated linoleic acid (CLA) concentrations (25, 50, 100 and 200 mumol/L) of c9, t11-CLA for 24 and 48h, with a negative control. The results showed that c9, t11-CLA can recover GJIC of CHL cell blocked by TPA and similar to negative control at 200 mumol/L 48 h of c9, t11-CLA; c9, t11-CLA also can recover cancer cells(SGC-7901 and MCF-7) at doses of 24 h 100 mumol/L for MCF-7 cells and 24 h 200 mumol/L, 48 h 100 mumol/L, 200 mumol/L for SGC-7901 and MCF-7 cells. It was suggested that c9, t11-CLA can recover GJIC of CHL cells blocked by TPA and SGC-7901 and MCF-7 cells.
Liu
Jiaren
J
Public Health College of Harbin Medical University, Harbin 150001, China.
Chen
Bingqing
B
Yang
Yanmei
Y
D000230:Adenocarcinoma / Q000473:pathology; D000818:Animals; D001943:Breast Neoplasms / Q000473:pathology; D002450:Cell Communication / Q000187:drug effects*; D002478:Cells, Cultured; D017629:Gap Junctions / Q000187:drug effects*; D006801:Humans; D007536:Isomerism; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology*; D008168:Lung / Q000166:cytology; D051379:Mice; D013274:Stomach Neoplasms / Q000473:pathology; D014407:Tumor Cells, Cultured
D044243:Linoleic Acids, Conjugated