title: Modulation of guinea pig intrinsic cardiac neurons by prostaglandins.
abstract: Activation of cardiac mast cells has been shown to alter parasympathetic neuronal function via the activation of histamine receptors. The present study examined the ability of prostaglandins to alter the activity of guinea pig intracardiac neurons. Intracellular voltage recordings from whole mounts of the cardiac plexus showed that antigen-mediated mast cell degranulation produces an attenuation of the afterhyperpolarization (AHP), which was prevented by the phospholipase A2 inhibitor 5,8,11,14-eicosatetraynoic acid. Exogenous application of either PGD2 or PGE2 produced a biphasic change in the membrane potential and an inhibition of both AHP amplitude and duration. Examination of prostanoid receptors using bath perfusions (1 microM PGE2 and PGD2), specific agonists (BW245C, sulprostone, and butaprost), and antagonists (AH6809 and SC19220) found evidence for both the PGE2-specific EP2 and EP3 receptors, but not for EP1 or the PGD2-specific prostanoid (DP) receptors. Sulprostone was able to mimic the PGE2 responses in some cells, but not in all PGE2-sensitive cells. Butaprost was able to mimic the PG-induced hyperpolarization in some cells, but did not alter the AHP. Inhibition of specific potassium channels with either TEA, charybdotoxin, or apamin showed that neither TEA nor charybdotoxin could prevent the PGE2-induced AHP attenuation. Apamin alone inhibited AHP duration, with PGs having no further effect in these cells. These results demonstrate that guinea pig intracardiac neurons can be modulated by PG, most likely through either EP2, EP3, or potentially EP4 receptors, and this response is due, at least in part, to a reduction in small-conductance KCa currents.
authors.affiliation: Dept. of Biology, Ithaca College, Ithaca, NY 14850, USA. jhardwick@ithaca.edu
authors.forename: Gregory S
authors.initials: GS
authors.lastname: Jelson
authors.forename: Gina M
authors.initials: GM
authors.lastname: DeMasi
authors.forename: Kristen L
authors.initials: KL
authors.lastname: Sager
authors.forename: Jean C
authors.initials: JC
authors.lastname: Hardwick
mesh_terms: D000818:Animals; D015232:Dinoprostone / Q000494:pharmacology*; D005260:Female; D005726:Ganglia, Parasympathetic / Q000187:drug effects* / Q000502:physiology*; D006168:Guinea Pigs; D006321:Heart / Q000294:innervation*; D006827:Hydantoins / Q000494:pharmacology; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology; D008564:Membrane Potentials / Q000187:drug effects; D010741:Phospholipases A / Q000037:antagonists & inhibitors; D054467:Phospholipases A2; D026902:Potassium Channel Blockers / Q000494:pharmacology; D015230:Prostaglandin D2 / Q000494:pharmacology*; D011982:Receptors, Prostaglandin / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism; D014966:Xanthenes / Q000494:pharmacology; D044004:Xanthones
chemical_list: D006827:Hydantoins; D026902:Potassium Channel Blockers; D011982:Receptors, Prostaglandin; D014966:Xanthenes; D044004:Xanthones; C053876:6-isopropoxy-9-oxoxanthene-2-carboxylic acid; C030669:BW 245C; D010741:Phospholipases A; D054467:Phospholipases A2; D015232:Dinoprostone; D015230:Prostaglandin D2