title: Study of the structural dynamics of the E coli 70S ribosome using real-space refinement.
abstract: Cryo-EM density maps showing the 70S ribosome of E. coli in two different functional states related by a ratchet-like motion were analyzed using real-space refinement. Comparison of the two resulting atomic models shows that the ribosome changes from a compact structure to a looser one, coupled with the rearrangement of many of the proteins. Furthermore, in contrast to the unchanged inter-subunit bridges formed wholly by RNA, the bridges involving proteins undergo large conformational changes following the ratchet-like motion, suggesting an important role of ribosomal proteins in facilitating the dynamics of translation.
authors.affiliation: Howard Hughes Medical Institute, Health Research, Inc, Empire State Plaza, Albany, NY 12201, USA.
authors.forename: Haixiao
authors.initials: H
authors.lastname: Gao
authors.forename: Jayati
authors.initials: J
authors.lastname: Sengupta
authors.forename: Mikel
authors.initials: M
authors.lastname: Valle
authors.forename: Andrei
authors.initials: A
authors.lastname: Korostelev
authors.forename: Narayanan
authors.initials: N
authors.lastname: Eswar
authors.forename: Scott M
authors.initials: SM
authors.lastname: Stagg
authors.forename: Patrick
authors.initials: P
authors.lastname: Van Roey
authors.forename: Rajendra K
authors.initials: RK
authors.lastname: Agrawal
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Harvey
authors.forename: Andrej
authors.initials: A
authors.lastname: Sali
authors.forename: Michael S
authors.initials: MS
authors.lastname: Chapman
authors.forename: Joachim
authors.initials: J
authors.lastname: Frank
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000648:ultrastructure; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D046911:Macromolecular Substances; D008958:Models, Molecular; D008968:Molecular Conformation; D008970:Molecular Weight; D014176:Protein Biosynthesis / Q000502:physiology; D020836:Protein Structure, Quaternary / Q000502:physiology*; D017434:Protein Structure, Tertiary / Q000502:physiology; D012313:RNA / Q000737:chemistry*; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000648:ultrastructure; D012270:Ribosomes / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D001426:Bacterial Proteins; D046911:Macromolecular Substances; D012269:Ribosomal Proteins; D012313:RNA