Assessment of bioavailability of soil-sorbed atrazine.
Bioavailability of pesticides sorbed to soils is an important determinant of their environmental fate and impact. Mineralization of sorbed atrazine was studied in soil and clay slurries, and a desorption-biodegradation-mineralization (DBM) model was developed to quantitatively evaluate the bioavailability of sorbed atrazine. Three atrazine-degrading bacteria that utilized atrazine as a sole N source (Pseudomonas sp. strain ADP, Agrobacterium radiobacter strain J14a, and Ralstonia sp. strain M91-3) were used in the bioavailability assays. Assays involved establishing sorption equilibrium in sterile soil slurries, inoculating the system with organisms, and measuring the CO(2) production over time. Sorption and desorption isotherm analyses were performed to evaluate distribution coefficients and desorption parameters, which consisted of three desorption site fractions and desorption rate coefficients. Atrazine sorption isotherms were linear for mineral and organic soils but displayed some nonlinearity for K-saturated montmorillonite. The desorption profiles were well described by the three-site desorption model. In many instances, the mineralization of atrazine was accurately predicted by the DBM model, which accounts for the extents and rates of sorption/desorption processes and assumes biodegradation of liquid-phase, but not sorbed, atrazine. However, for the Houghton muck soil, which manifested the highest sorbed atrazine concentrations, enhanced mineralization rates, i.e., greater than those expected on the basis of aqueous-phase atrazine concentration, were observed. Even the assumption of instantaneous desorption could not account for the elevated rates. A plausible explanation for enhanced bioavailability is that bacteria access the localized regions where atrazine is sorbed and that the concentrations found support higher mineralization rates than predicted on the basis of aqueous-phase concentrations. Characteristics of high sorbed-phase concentration, chemotaxis, and attachment of cells to soil particles seem to contribute to the bioavailability of soil-sorbed atrazine.
Park
Jeong-Hun
JH
Department of Civil and Environmental Engineering, Michigan State University, East Lansing, Michigan 48824, USA.
Feng
Yucheng
Y
Ji
Pingsheng
P
Voice
Thomas C
TC
Boyd
Stephen A
SA
D001280:Atrazine / Q000737:chemistry* / Q000493:pharmacokinetics*; D020562:Betaproteobacteria / Q000378:metabolism; D001673:Biodegradation, Environmental; D001682:Biological Availability; D006090:Gram-Negative Bacteria / Q000378:metabolism*; D006540:Herbicides / Q000493:pharmacokinetics*; D008954:Models, Biological; D011549:Pseudomonas / Q000378:metabolism; D012231:Rhizobium / Q000378:metabolism; D012987:Soil / Q000032:analysis*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism
D006540:Herbicides; D012987:Soil; D012989:Soil Pollutants; D001280:Atrazine