Quantitative proteome analysis: methods and applications.
With the completion of the genomic sequencing of a number of species, including that of humans, much attention is currently focused on how the information in these sequences might be interpreted in terms of the structure, function, and control of biologic systems and processes. Quantitative proteome analysis, the global analysis of protein expression, is increasingly being used as a method to study steady-state and perturbation-induced changes in protein profiles. The rationale for quantitative proteome analysis is described, along with a new technology for high throughput quantitative profiling of proteins in complex mixtures and its current status with selected applications.
Aebersold
Ruedi
R
Institute for Systems Biology, Seattle, Washington 98103, USA. raebersold@systemsbiology.org
D000345:Affinity Labels; D000818:Animals; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*
D000345:Affinity Labels; D008565:Membrane Proteins; D020543:Proteome