Ionoregulatory disruption as the acute toxic mechanism for lead in the rainbow trout (Oncorhynchus mykiss).
The mechanism for acute toxicity of lead (Pb) in rainbow trout (Oncorhynchus mykiss) was investigated at Pb concentrations close to the 96 h LC50 of 1.0 mg dissolved Pb l(-1) (0.8-1.4, 95% C.I.) determined in dechlorinated Hamilton city tap water (from Lake Ontario, hardness=140 mg l(-1) CaCO(3)). Tissue Pb accumulation associated with death was highest in the gill, followed by kidney and liver. Significant ionoregulatory impacts were observed in adult rainbow trout (200-300 g) fitted with indwelling dorsal aortic catheters and exposed to 1.1+/-0.04 mg dissolved Pb l(-1). Decreased plasma [Ca(2+)], [Na(+)] and [Cl(-)] occurred after 48 h of exposure through to 120 h, with increases in plasma [Mg(2+)], ammonia, and cortisol. No marked changes in PaO(2), PaCO(2), pH, glucose, or hematological parameters were evident. Branchial Na(+)/K(+) ATPase activity in juvenile trout exposed to concentrations close to the 96 h LC50 was inhibited by approximately 40% after 48 h of Pb exposure. Calcium ion flux measurements using 45Ca as a radiotracer showed 65% inhibition of Ca(2+) influx after 0, 12, 24 or 48 h exposure to the 96 h LC50 concentration of Pb. There was also significant inhibition (40-50%) of both Na(+) and Cl(-) uptake, measured with 22Na and 36Cl simultaneously. We conclude that the mechanism of acute toxicity for Pb in rainbow trout occurs by ionoregulatory disruption rather than respiratory or acid/base distress at Pb concentrations close to the 96 h LC50 in moderately hard water.
Rogers
J T
JT
Department of Biology, McMaster University, 1280 Main Street West, Hamilton, Ont., Canada L8S 4K1. rogersjt@mcmaster.ca
Richards
J G
JG
Wood
C M
CM
D000136:Acid-Base Equilibrium / Q000187:drug effects; D000818:Animals; D002118:Calcium / Q000097:blood; D005880:Gills / Q000187:drug effects / Q000378:metabolism; D017136:Ion Transport / Q000187:drug effects*; D007854:Lead / Q000493:pharmacokinetics / Q000633:toxicity*; D007928:Lethal Dose 50; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D011188:Potassium / Q000097:blood; D014018:Tissue Distribution; D023382:Toxicity Tests, Acute; D014873:Water Pollutants / Q000493:pharmacokinetics / Q000633:toxicity*
D014873:Water Pollutants; D007854:Lead; D011188:Potassium; D002118:Calcium