Cell array coupled with laser scanning cytometry allows easy analysis of changes in cyclin expression during the cell cycle. An application of cell array system.
To assess the potential of cell array technology, a cell array slide with 50 spots was designed specifically for automated analysis of changes in expression of cyclins A and B1 during the cell cycle at the cellular level. Cells harvested every 1 hour from 0 through 23 hours after synchronization by mitotic selection were spotted in duplicate on a glass slide. Each slide contained 48 spots representing 24 different cell cycle phases, and the remaining two spots were peripheral blood lymphocytes, which were included as negative control. This cell array provided temporal and spatial information related to changes in cyclin expression during the cell cycle in a single experiment. The present study indicates that expression analysis by cell array is novel approach for cell cycle studies. Furthermore, sophisticated multiplexed cell array technology has great potential for analyses of expression of specific genes during diverse cellular events at the cellular level.
Furuya
Tomoko
T
Department of Pathology, Yamaguchi University School of Medicine, Ube 755-8505, Japan.
Takita
Morihito
M
Tsunoda
Shin-ichi
S
Kawauchi
Shigeto
S
Hirano
Takashi
T
Oga
Atsunori
A
Sasaki
Kohsuke
K
D002453:Cell Cycle; D019925:Cyclin A / Q000032:analysis / Q000378:metabolism; D019926:Cyclin B / Q000032:analysis / Q000378:metabolism; D016213:Cyclins / Q000378:metabolism*; D019044:Image Cytometry / Q000379:methods*; D007834:Lasers
D019925:Cyclin A; D019926:Cyclin B; D016213:Cyclins