Ozone sensitivity of Fagus sylvatica and Fraxinus excelsior young trees in relation to leaf structure and foliar ozone uptake.
During the summer of 2001, 2-year-old Fraxinus excelsior and Fagus sylvatica plants were subjected to ozone-rich environmental conditions at the Regional Forest Nursery at Curno (Northern Italy). Atmospheric ozone concentrations and stomatal conductance were measured, in order to calculate the foliar fluxes by means of a one-dimensional model. The foliar structure of both species was examined (thickness of the lamina and of the individual tissues, leaf mass per area, leaf density) and chlorophyll a fluorescence was determined as a response parameter. Stomatal conductance was always greater in Fraxinus excelsior, as was ozone uptake, although the highest absorption peaks did not match the peaks of ozone concentration in the atmosphere. The foliar structure can help explain this phenomenon: Fraxinus excelsior has a thicker mesophyll than Fagus sylvatica (indicating a greater photosynthesis potential) and a reduced foliar density. This last parameter, related to the apoplastic fraction, suggests a greater ability to disseminate the gases within the leaf as well as a greater potential detoxifying capacity. As foliar symptoms spread, the parameters relating to chlorophyll a fluorescence also change. PI (Performance Index, Strasser, A., Srivastava, A., Tsimilli-Michael, M., 2000. The fluorescence transient as a tool to characterize and screen photosynthetic samples. In: Yunus, M., Pathre, U., Mohanty, P., (Eds.) Probing Photosynthesis: Mechanisms, Regulation and Adaptation. Taylor & Francis, London, UK, pp. 445-483.) has proved to be a more suitable index than Fv/Fm (Quantum Yield Efficiency) to record the onset of stress conditions.
Gerosa
Giacomo
G
Dipartimento di Produzione Vegetale, Università degli Studi di Milano, Via Celoria 2, 20133, Milan, Italy.
Marzuoli
Riccardo
R
Bussotti
Filippo
F
Pancrazi
Marica
M
Ballarin-Denti
Antonio
A
D000393:Air Pollutants / Q000009:adverse effects*; D004784:Environmental Monitoring / Q000379:methods; D029964:Fagus / Q000187:drug effects*; D031661:Fraxinus / Q000187:drug effects*; D045342:Light-Harvesting Protein Complexes; D010126:Ozone / Q000009:adverse effects*; D045322:Photosynthetic Reaction Center Complex Proteins / Q000187:drug effects; D018515:Plant Leaves / Q000187:drug effects / Q000502:physiology
D000393:Air Pollutants; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D010126:Ozone