title: Specific quantification of human genomes from low copy number DNA samples in forensic and ancient DNA studies.
abstract: We reviewed the current methodologies used for human DNA quantitation in forensic and ancient DNA studies, including sensitive hybridization methods based on the detection of nuclear alpha-satellite repetitive DNA regions or more recently developed fluorogenic real-time polymerase chain reaction (PCR) designs for the detection of both nuclear and mitochondrial DNA regions. Special emphasis has been put on the applicability of recently described different real-time PCR designs targeting different fragments of the HV1 mtDNA control region, and a segment of the X-Y homologous amelogenin gene. The importance of these quantitative assays is to ensure the consistency of low copy number DNA typing (STR profiling and mtDNA sequencing).
authors.affiliation: Instituto Nacional de Toxicología, Servicio de Biología, Luis Cabrera 9, 28002 Madrid, Spain. a.alonso@mju.es
authors.forename: Antonio
authors.initials: A
authors.lastname: Alonso
authors.forename: Pablo
authors.initials: P
authors.lastname: Martin
authors.forename: Cristina
authors.initials: C
authors.lastname: Albarrán
authors.forename: Pilar
authors.initials: P
authors.lastname: García
authors.forename: Dragan
authors.initials: D
authors.lastname: Primorac
authors.forename: Oscar
authors.initials: O
authors.lastname: García
authors.forename: Lourdes
authors.initials: L
authors.lastname: Fernández de Simón
authors.forename: Julia
authors.initials: J
authors.lastname: García-Hirschfeld
authors.forename: Manuel
authors.initials: M
authors.lastname: Sancho
authors.forename: Jose
authors.initials: J
authors.lastname: Fernández-Piqueras
mesh_terms: D053523:Amelogenin; D022801:Complementarity Determining Regions; D016172:DNA Fingerprinting / Q000379:methods*; D004272:DNA, Mitochondrial / Q000235:genetics; D003746:Dental Enamel Proteins / Q000235:genetics; D018732:Forensic Anthropology / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D012732:Sex Determination Analysis / Q000379:methods; D020080:Tandem Repeat Sequences
chemical_list: D053523:Amelogenin; D022801:Complementarity Determining Regions; D004272:DNA, Mitochondrial; D003746:Dental Enamel Proteins