Regeneration of the Egyptian medicinal plant Artemisia judaica L.
An in vitro propagation system for Artemisia judaica L., a traditional Egyptian medicinal plant, has been developed. De novo shoot organogenesis was induced by culturing etiolated hypocotyls and intact seedlings on medium supplemented with thidiazuron [N-phenyl-N'-(1,2,3-thidiazol-yl) urea] via callusing at the cotyledonary notch region. Up to 16 shoots formed per seedling cultured on a medium containing 1 micro mol l(-1) thidiazuron for an optimal duration of exposure of 20 days. Regenerated shoots formed roots when subcultured onto a medium containing 1 micromol l(-1) indole-3-butyric acid. The regeneration protocol developed in this study provides a basis for germplasm conservation and for further investigation of medicinally active constituents of A. judaica.
Liu
C Z
CZ
Department of Plant Agriculture, Biotechnology Division, Edmund C. Bovey Complex, University of Guelph, N1G 2W1 Guelph, Ontario, Canada.
Murch
S J
SJ
EL-Demerdash
M
M
Saxena
P K
PK
D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D019306:Artemisia / Q000187:drug effects / Q000196:embryology / Q000502:physiology*; D001593:Benzyl Compounds; D046508:Culture Techniques; D007701:Kinetin; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D010671:Phenylurea Compounds / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018520:Plant Shoots / Q000187:drug effects / Q000196:embryology / Q000502:physiology; D010946:Plants, Medicinal / Q000187:drug effects / Q000196:embryology / Q000502:physiology*; D011687:Purines; D012038:Regeneration / Q000187:drug effects; D013830:Thiadiazoles
D001593:Benzyl Compounds; D009280:Naphthaleneacetic Acids; D010671:Phenylurea Compounds; D010937:Plant Growth Regulators; D011687:Purines; D013830:Thiadiazoles; C016785:thidiazuron; C034182:1-naphthaleneacetic acid; D000225:Adenine; C480551:benzylaminopurine; D007701:Kinetin