title: Expression of Notch1 and Jagged2 in the enteric nervous system.
abstract: The Notch signaling pathway is a vitally important pathway in regulating brain development. To explore the involvement of the Notch pathway in neuronal cells of adult rat gut, we investigated the expression of Notch1 and Jagged2 by in situ hybridization (ISH) and immunohistochemistry (IHC). In the enteric nervous system, Notch1 and Jagged2 were expressed in ganglia of the submucosal and myenteric plexus. Notch1 was preferentially expressed in cholinergic neurons lacking calretinin or nitric oxide synthase (NOS), whereas Jagged2 was present in most neuron subtypes. We propose that Notch1 and Jagged2 have a continuing role in the maintenance and function of neuronal cells in the adult enteric nervous system.
authors.affiliation: Child Health Research Institute, Womens and Childrens Hospital, 72 King William Road, North Adelaide, South Australia 5006, Australia. guy.sander@adelaide.edu.au
authors.forename: Guy R
authors.initials: GR
authors.lastname: Sander
authors.forename: Simon J H
authors.initials: SJ
authors.lastname: Brookes
authors.forename: Barry C
authors.initials: BC
authors.lastname: Powell
mesh_terms: D000818:Animals; D064032:Calbindin 2; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins / Q000378:metabolism*; D017615:Enteric Nervous System / Q000033:anatomy & histology / Q000378:metabolism*; D005260:Female; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D036341:Intercellular Signaling Peptides and Proteins; D007413:Intestinal Mucosa / Q000294:innervation / Q000378:metabolism; D000072101:Jagged-2 Protein; D008565:Membrane Proteins / Q000378:metabolism*; D009197:Myenteric Plexus / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D051881:Receptor, Notch1; D051883:Receptor, Notch2; D011956:Receptors, Cell Surface / Q000378:metabolism; D064030:S100 Calcium Binding Protein G / Q000378:metabolism; D000072099:Serrate-Jagged Proteins; D013368:Submucous Plexus / Q000378:metabolism; D014157:Transcription Factors
chemical_list: C576512:Calb2 protein, rat; D064032:Calbindin 2; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D036341:Intercellular Signaling Peptides and Proteins; C490502:Jag2 protein, rat; D000072101:Jagged-2 Protein; D008565:Membrane Proteins; C497763:Notch1 protein, rat; C497766:Notch2 protein, rat; D011506:Proteins; D051881:Receptor, Notch1; D051883:Receptor, Notch2; D011956:Receptors, Cell Surface; D064030:S100 Calcium Binding Protein G; D000072099:Serrate-Jagged Proteins; D014157:Transcription Factors; D019001:Nitric Oxide Synthase