Study of the structural dynamics of the E coli 70S ribosome using real-space refinement.
Cryo-EM density maps showing the 70S ribosome of E. coli in two different functional states related by a ratchet-like motion were analyzed using real-space refinement. Comparison of the two resulting atomic models shows that the ribosome changes from a compact structure to a looser one, coupled with the rearrangement of many of the proteins. Furthermore, in contrast to the unchanged inter-subunit bridges formed wholly by RNA, the bridges involving proteins undergo large conformational changes following the ratchet-like motion, suggesting an important role of ribosomal proteins in facilitating the dynamics of translation.
Gao
Haixiao
H
Howard Hughes Medical Institute, Health Research, Inc, Empire State Plaza, Albany, NY 12201, USA.
Sengupta
Jayati
J
Valle
Mikel
M
Korostelev
Andrei
A
Eswar
Narayanan
N
Stagg
Scott M
SM
Van Roey
Patrick
P
Agrawal
Rajendra K
RK
Harvey
Stephen C
SC
Sali
Andrej
A
Chapman
Michael S
MS
Frank
Joachim
J
D001426:Bacterial Proteins / Q000737:chemistry* / Q000648:ultrastructure; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D046911:Macromolecular Substances; D008958:Models, Molecular; D008968:Molecular Conformation; D008970:Molecular Weight; D014176:Protein Biosynthesis / Q000502:physiology; D020836:Protein Structure, Quaternary / Q000502:physiology*; D017434:Protein Structure, Tertiary / Q000502:physiology; D012313:RNA / Q000737:chemistry*; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000648:ultrastructure; D012270:Ribosomes / Q000737:chemistry* / Q000648:ultrastructure
D001426:Bacterial Proteins; D046911:Macromolecular Substances; D012269:Ribosomal Proteins; D012313:RNA