Association of human kinesin superfamily protein member 4 with BRCA2-associated factor 35.
A large portion of human kinesin superfamily protein member 4 (KIF4) is associated with the nuclear matrix during the interphase, while a small portion is found in the cytoplasm. During mitosis, it is associated with chromosomes throughout the entire process. In the present study, we identified a protein that interacts with KIF4 using a yeast two-hybrid system, co-immunoprecipitation and co-fractionation. This protein is BRCA2-associated factor 35 (BRAF35) containing a non-specific DNA binding high-mobility-group domain and a kinesin-like coiled-coil domain. It appeared that the interaction between the two proteins occurs through their respective alpha-helical coiled-coil domains. The co-fractionation experiment revealed that KIF4 and BRAF35 were present in a complex of approx. 540 kDa. The composition and biological significance of this complex should be studied further.
Lee
Young Mi
YM
Institute for Medical Sciences, School of Medicine, Ajou University, Suwon 442-749, South Korea.
Kim
Wankee
W
D000595:Amino Acid Sequence; D024682:BRCA2 Protein / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D015723:Gene Library; D005822:Genetic Vectors / Q000235:genetics; D006367:HeLa Cells; D006609:High Mobility Group Proteins; D006801:Humans; D016547:Kinesins / Q000737:chemistry / Q000378:metabolism*; D016350:Leucine Zippers; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics; D010455:Peptides / Q000378:metabolism; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014407:Tumor Cells, Cultured; D020798:Two-Hybrid System Techniques
D024682:BRCA2 Protein; D004268:DNA-Binding Proteins; C000625244:HMG20B protein, human; D006609:High Mobility Group Proteins; D009363:Neoplasm Proteins; D010455:Peptides; C484228:KIF4A protein, human; C484229:KIF4B protein, human; D016547:Kinesins