Severe reduction of superoxide dismutase activity in the yeast Saccharomyces cerevisae with the deletion or overexpression of GTS1.
We report herein that the level of reactive oxygen species (ROS) observed using dihydrorhodamine is much higher in either GTS1-deleted (gts1Delta) or GTS1-overexpressing (TMpGTS1) transformants than in the wild-type and that the levels of protein carbonyls are increased and the glutathione levels are decreased in both transformants. Consistently, the activities of superoxide dismutases (SODs) in both gts1Delta and TMpGTS1 were severely weakened, while the protein levels of both Cu/Zn-SOD and Mn-SOD were not so changed. As the intracellular copper levels were significantly increased in both transformants, we hypothesized that, in either gts1Delta or TMpGTS1 cells, the imbalanced homeostasis of copper induced an accumulation of ROS which caused inactivation of SODs further increasing ROS levels.
Abudugupur
Abudokirim
A
Department of Biochemistry 2, University of Yamanashi, Faculty of Medicine, 1110 Shimokato, Tamaho, Nakakoma, Yamanashi 409-3898, Japan.
Xu
Zhaojun
Z
Mitsui
Kazuhiro
K
Hisaki
Harumi
H
Ueda
Nobuo
N
Amemiya
Tatsumi
T
Tsurugi
Kunio
K
D003300:Copper / Q000378:metabolism; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D017382:Reactive Oxygen Species / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D013482:Superoxide Dismutase / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics*
C097841:GTS1 protein, S cerevisiae; D017382:Reactive Oxygen Species; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D003300:Copper; D013482:Superoxide Dismutase