Polymeric FAD used as enzyme-friendly mediator in lactate detection.
We present the fabrication and properties of lactate biosensors. The novel feature is the use of polymerized flavin adenine dinucleotide (FAD) as mediator for electron transfer. The biosensors were prepared using lactate dehydrogenase (LDH), lactate oxidase (LOX), or baker's yeast (BY) immobilized at the surface of the electrode. The sensors using purified enzymes showed good sensitivity, linearity, and stability. The sensitivity of the BY electrodes was slightly lower. The advantages of this type of sensors are discussed in connection with potential applications.
Leonida
Mihaela D
MD
Fairleigh Dickinson University, SONS, H444 N, Teaneck, NJ 07666, USA. mleonida@fdu.edu
Starczynowski
Daniel T
DT
Waldman
Riva
R
Aurian-Blajeni
Benedict
B
D001704:Biopolymers / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D019344:Lactic Acid / Q000032:analysis*; D006899:Mixed Function Oxygenases / Q000378:metabolism; D015003:Yeasts
D001704:Biopolymers; D004800:Enzymes, Immobilized; D005182:Flavin-Adenine Dinucleotide; D019344:Lactic Acid; D006899:Mixed Function Oxygenases; D007770:L-Lactate Dehydrogenase; C021497:lactate 2-monooxygenase