title: Antibacterial activity of bactericide-immobilized filler for resin-based restoratives.
abstract: This study examined the antibacterial activity of prepolymerized resin filler, in which the bactericide quaternary ammonium was immobilized. The experimental filler was prepared by grinding prepolymerized resin blocks of methacrylate monomers, silica particles, and the antibacterial monomer methacryloyloxydodecylpyridinium bromide (MDPB). The number of Streptococcus mutans after incubation for 18h in contact with the experimental filler with or without protein adsorption by saliva treatment was determined, and adherence of bacteria to the filler surface was evaluated by scanning electron microscopy. Elution of unpolymerized MDPB from the filler and its influence on bacterial growth were also investigated. The growth of S. mutans was completely inhibited by contact with the experimental filler without saliva treatment. Although the effects were attenuated, the saliva-treated filler still exhibited growth inhibition at >99.9%. Less bacteria attached to the experimental filler than the control filler without MDPB, indicating that the reduction in bacterial number after contact with the experimental filler was not due to bacterial adherence to the particles. Unpolymerized MDPB at 1 microg/ml was eluted from the filler particles but was confirmed to have little effect on bacterial growth. The results indicate that the bactericide-immobilized filler containing MDPB shows significant bacteriostatic effects without releasing antibacterial components, and is useful for incorporation into various resin-based restoratives.
authors.affiliation: Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan. imazato@dent.osaka-u.ac.jp
authors.forename: Satoshi
authors.initials: S
authors.lastname: Imazato
authors.forename: Noboru
authors.initials: N
authors.lastname: Ebi
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Kaneko
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Ebisu
authors.forename: Roy R B
authors.initials: RR
authors.lastname: Russell
mesh_terms: D000327:Adsorption; D000891:Anti-Infective Agents, Local / Q000378:metabolism*; D001422:Bacterial Adhesion; D003188:Composite Resins / Q000737:chemistry / Q000378:metabolism*; D003793:Dental Restoration, Permanent; D006801:Humans; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D011726:Pyridinium Compounds / Q000378:metabolism*; D012463:Saliva / Q000378:metabolism; D013295:Streptococcus mutans / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D000891:Anti-Infective Agents, Local; D003188:Composite Resins; D008689:Methacrylates; D011726:Pyridinium Compounds; C089392:methacryloyloxydodecylpyridinium bromide