[Effect of tumor suppressor gene KAI1 on the biological behaviors of human colorectal carcinoma].
OBJECTIVE
To determine whether the expression of tumor suppressor gene KAI1 can suppress the invasive or metastatic ability of colorectal carcinoma cells.


METHODS
KAI1 cDNA was transfected into highly malignant colorectal carcinoma cell line LoVo, which had low levels of endogenous KAI1 expression. The expressions of KAI1 mRNA and protein were determined by immunohistochemistry and in situ hybridization, and the biological behaviors of the KAI1-transfected LoVo cells were investigated by cell-cell adhesion, cell-matrix adhesion and invasion assays.


RESULTS
KAI1 expression significantly suppressed the metastatic potential of KAI1-transfected LoVo cells, whose metastasis suppression was correlated with the increased adhesion to homotypic cells and reduced tumor adhesion to extracellular matrix components. KAI1 expression significantly suppressed the in vitro cell invasion in KAI1-transfected LoVo cells.


CONCLUSIONS
KAI1 might function as inhibitor of colorectal carcinoma metastasis.
Liu
Li
L
Department of Pathology, First Military Medical University, Guangzhou 510515, China.
Li
Zu-guo
ZG
Yang
Guang-zhi
GZ
Ding
Yan-qing
YQ
D000818:Animals; D015703:Antigens, CD; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000473:pathology*; D016147:Genes, Tumor Suppressor / Q000502:physiology*; D006801:Humans; D051861:Kangai-1 Protein; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000502:physiology; D051379:Mice; D041681:NIH 3T3 Cells; D009362:Neoplasm Metastasis / Q000517:prevention & control*; D011518:Proto-Oncogene Proteins; D014162:Transfection
D015703:Antigens, CD; C497688:CD82 protein, human; C497689:Cd82 antigen, mouse; D051861:Kangai-1 Protein; D008562:Membrane Glycoproteins; D011518:Proto-Oncogene Proteins