Efficacy of a larger version of the hybrid artificial liver support system using a polyurethane foam/spheroid packed-bed module in a warm ischemic liver failure pig model for preclinical experiments.
We have reported the usefulness of a polyurethane foam packed-bed culture system of hepatocyte spheroids as a hybrid artificial liver support system (PUF-HALSS). The aim of this study was to evaluate in detail the efficacy in serum parameters regarding the liver function of a larger version of the PUF-HALSS containing 2 x 10(10) porcine hepatocytes for clinical use in warm ischemic liver failure pigs. Warm ischemic liver failure pigs weighing 25 kg were divided into two groups: (1) a control group (n = 3), in which each pig was attached to a PUF-HALSS without hepatocytes, and (2) a HALSS group (n = 3), in which each pig was attached to a PUF-HALSS. In the HALSS group, the increase of blood ammonia was completely suppressed and blood lactate levels were significantly suppressed. The Fisher's ratio was better maintained, and the increase of total bile acid, glycochenodeoxycholic acid, and taurochenodeoxycholic acid was significantly suppressed in the HALSS group. Serum creatinine levels were significantly lower, and blood glucose levels were significantly higher in the HALSS group. Serum levels of tumor necrosis factor- a were not elevated in either group. In conclusion, the larger version of the PUF-HALSS demonstrated many advantages as a liver support system in warm ischemic liver failure pigs.
Yamashita
Yo-ichi
Y
Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka 812-8582, Japan. yamashi@surg2.med.kyushu-u.ac.jp
Shimada
Mitsuo
M
Tsujita
Eiji
E
Shirabe
Ken
K
Ijima
Hiroyuki
H
Nakazawa
Kohji
K
Sakiyama
Ryoichi
R
Fukuda
Junji
J
Funatsu
Kazumori
K
Sugimachi
Keizo
K
D000596:Amino Acids / Q000097:blood; D000641:Ammonia / Q000097:blood; D000818:Animals; D001647:Bile Acids and Salts / Q000097:blood; D046508:Culture Techniques / Q000379:methods*; D005112:Extracorporeal Circulation / Q000295:instrumentation / Q000379:methods; D005260:Female; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism; D006801:Humans; D007511:Ischemia / Q000628:therapy*; D019344:Lactic Acid / Q000097:blood; D017093:Liver Failure / Q000628:therapy*; D019164:Liver, Artificial; D011140:Polyurethanes / Q000378:metabolism*; D018874:Spheroids, Cellular / Q000378:metabolism*; D013552:Swine; D013696:Temperature
D000596:Amino Acids; D001647:Bile Acids and Salts; D011140:Polyurethanes; D019344:Lactic Acid; D000641:Ammonia; C028279:polyurethane foam