RA175, which is the mouse ortholog of TSLC1, a tumor suppressor gene in human lung cancer, is a cell adhesion molecule.
RA175, a new immunoglobulin superfamily member, is preferentially expressed during differentiation of P19 embryonic carcinoma (EC) cells induced by retinoic acid. In the present study, we isolated mouse RA175 cDNA in its entirety and showed that RA175 is the mouse ortholog of TSLC1, a tumor suppressor gene in human lung cancer. RA175/TSLC1 was localized in the adherent region of human lung squamous carcinoma cells and in the differentiated P19 EC cells. RA175/TSLC1 showed homophilic trans-interaction activity in a Ca(2+)-independent manner. RA175/TSLC1 was preferentially expressed in the polarized cells lining the lumen of developing mouse lung epithelium. This suggests that RA175/TSLC1 is a cell adhesion molecule that is acting as a tumor suppressor gene in the metastasis of lung tumors. RA175/TSLC1 may be necessary for cells to remain tightly associated in the epithelium, thereby suppressing metastasis.
Fujita
Eriko
E
Division of Development and Differentiation, National Institute of Neuroscience, NCNP, 187-8502, Kodaira, Tokyo, Japan.
Soyama
Akiko
A
Momoi
Takashi
T
D000818:Animals; D015820:Cadherins / Q000378:metabolism; D020013:Calcium Signaling / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism; D002448:Cell Adhesion / Q000235:genetics*; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D002451:Cell Compartmentation / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics / Q000302:isolation & purification*; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D000075983:Nectins; D009362:Neoplasm Metastasis / Q000235:genetics*; D010750:Phosphoproteins / Q000378:metabolism; D017434:Protein Structure, Tertiary / Q000235:genetics; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D020545:Respiratory Mucosa / Q000378:metabolism* / Q000473:pathology; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000302:isolation & purification*; D062826:Zonula Occludens-1 Protein
C403514:CADM1 protein, human; D015820:Cadherins; C498598:Cadm1 protein, mouse; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules; D018076:DNA, Complementary; D007136:Immunoglobulins; D008565:Membrane Proteins; D000075983:Nectins; D010750:Phosphoproteins; D011506:Proteins; C569042:TJP1 protein, human; C569043:Tjp1 protein, mouse; D025521:Tumor Suppressor Proteins; D062826:Zonula Occludens-1 Protein; D014212:Tretinoin