Matrix protein gene sequence analysis of avian paramyxovirus 1 isolates obtained from pigeons.
The matrix protein gene was cloned and sequenced for several recent isolates of avian paramyxovirus type 1 (APMV-1). Specifically, isolates from pigeons and doves, members of the Columbidae family were examined. APMV-1 is the causative agent of Newcastle disease and the virus is associated with disease among a diverse number of avian species. Newcastle disease virus (NDV) isolates from pigeons have also been classified as pigeon paramyxovirus type 1 (PPMV-1). Matrix protein gene sequences for PPMV-1 isolates clustered together as a group relative to isolates from other species phylogenetically. However, there were also isolates from pigeons or doves that grouped with APMV-1 isolates from other species. This indicates that PPMV-1 may be circulating among Columbidae members as a distinct lineage, but that these avian species may also harbor other NDV strains as well. Of particular interest was a dove isolate from Europe that had an aberrant fusion protein cleavage site and was an outlying member phylogenetically between the two major groups of APMV-1 isolates.
Zanetti
Flavia
F
Instituto de Biotecnología, Centro de Investigaciones en Ciencias Veterinarias y Agropecuarias, Instituto Nacional de Tecnología Agropecuaria, Castelar, Buenos Aires, Argentina.
Rodríguez
Marianela
M
King
Daniel J
DJ
Capua
Ilaria
I
Carrillo
Elisa
E
Seal
Bruce S
BS
Berinstein
Analía
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D010856:Columbidae / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009522:Newcastle disease virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D014760:Viral Fusion Proteins / Q000235:genetics; D014763:Viral Matrix Proteins / Q000235:genetics*
D004279:DNA, Viral; D014760:Viral Fusion Proteins; D014763:Viral Matrix Proteins