Purification of peroxisomes using a density barrier in a swinging-bucket rotor.
In iodixanol, peroxisomes are the densest organelle in the light mitochondrial fraction and are therefore easily separated from the other components (lysosomes, mitochondria, etc.) in a preformed isosmotic continuous gradient. Because of the large difference in density between peroxisomes and the next densest organelle (mitochondria), a density barrier is effective. The resolution of the peroxisomes is far superior than that in sucrose and, unlike in Percoll, there is no contamination from endoplasmic reticulum.
Graham
John M
JM
School of Biomolecular Sciences, Liverpool John Moores University. john@jgrescon.fsbusiness.co.uk
D000818:Animals; D002500:Centrifugation, Isopycnic / Q000295:instrumentation* / Q000379:methods*; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology; D008099:Liver / Q000737:chemistry; D008297:Male; D015388:Organelles / Q000201:enzymology / Q000378:metabolism; D020675:Peroxisomes / Q000201:enzymology / Q000378:metabolism*; D051381:Rats; D013347:Subcellular Fractions / Q000737:chemistry / Q000201:enzymology; D014283:Triiodobenzoic Acids / Q000737:chemistry / Q000494:pharmacology
D004791:Enzyme Inhibitors; D014283:Triiodobenzoic Acids; C044834:iodixanol