title: Membrane cartridges for endotoxin removal from interferon preparations.
abstract: The suitability of membrane cartridges for the removal of endotoxin from both distilled water and interferon preparations was examined. The endotoxin concentrations were reduced to 4.0 and 7.3 EU/ml, respectively, when about 4000 ml of distilled water with 20 and 28 EU/ml were passed through the deoxycholate and chitosan immobilized membrane cartridges. When 200 ml of interferon preparation with endotoxin concentration more than 80 EU/ml and pH 3.9 were applied to a deoxycholate immobilized membrane cartridge at a flow-rate of 9 ml/min, the endotoxin concentration was reduced to less than 10 EU/ml. However, if an interferon preparation of 450 ml, with more than 80 EU/ml of endotoxin and pH 3.9 was applied to the chitosan immobilized membrane cartridge at a flow-rate of 18 ml/min, the endotoxin concentration was reduced to less than 10 EU/ml.
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 161 Zhongshan Road, Dalian 116012, PR China.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Li
authors.forename: Yingguang
authors.initials: Y
authors.lastname: Shao
authors.forename: Zhaoan
authors.initials: Z
authors.lastname: Chen
authors.forename: Runzi
authors.initials: R
authors.lastname: Cong
authors.forename: Junde
authors.initials: J
authors.lastname: Wang
authors.forename: Xueliang
authors.initials: X
authors.lastname: Liu
mesh_terms: D000327:Adsorption; D004731:Endotoxins / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007372:Interferons / Q000737:chemistry*; D008567:Membranes, Artificial
chemical_list: D004731:Endotoxins; D008567:Membranes, Artificial; D007372:Interferons