title: Loss of 1p and 7p in radiation-induced meningiomas identified by comparative genomic hybridization.
abstract: Cytogenetic and molecular studies of radiation-induced meningiomas (RIM) are rare and controversial. While comparative genomic hybridization (CGH) analysis identified monosomy 22 as the predominant change in RIM, occurring in frequencies comparable to those found in spontaneous meningioma (SM), molecular genetic analysis shows infrequent loss of chromosome 22 DNA markers. We have performed CGH analysis of six additional cases of RIM and detected an unbalanced genome in five of 6 cases. Loss of 1p and 7p was identified in the majority of RIM with an abnormal karyotype (4/5 cases), whereas loss of 6q occurred in three of five cases. Only one of five RIM had monosomy for chromosome 22. Loss of 7p is not frequently reported in SM and yet it was detected in four of 5 RIM with an abnormal karyotype in our study. Molecular and cytogenetic studies of chromosome 7 copy number should be attempted on a larger number of RIM to further investigate the role of 7p loss in RIM.
authors.affiliation: Cytogenetics Laboratory, Department of Pathology, University of British Columbia, BC Children's Hospital, Vancouver, BC, Canada. eseparovic@cw.bc.ca
authors.forename: Evica
authors.initials: E
authors.lastname: Rajcan-Separovic
authors.forename: John
authors.initials: J
authors.lastname: Maguire
authors.forename: Tatiana
authors.initials: T
authors.lastname: Loukianova
authors.forename: Meimun
authors.initials: M
authors.lastname: Nisha
authors.forename: Dagmar
authors.initials: D
authors.lastname: Kalousek
mesh_terms: D000328:Adult; D000368:Aged; D001483:Base Sequence; D002872:Chromosome Deletion; D002878:Chromosomes, Human, Pair 1; D002897:Chromosomes, Human, Pair 7; D017931:DNA Primers; D005260:Female; D006801:Humans; D008297:Male; D008579:Meningioma / Q000235:genetics*; D008875:Middle Aged; D009381:Neoplasms, Radiation-Induced / Q000235:genetics*; D009693:Nucleic Acid Hybridization / Q000379:methods*
chemical_list: D017931:DNA Primers