Overlapping omt1+ and omt2+ genes are required for spore wall maturation in Schizosaccharomyces pombe.
BACKGROUND
Overlapping genes that are transcribed from the same genomic regions are rare in eukaryotes and to date few detailed functional analyses have been reported.


RESULTS
We report here three novel overlapping transcripts that are specifically expressed during meiosis of Schizosaccharomyces pombe. They are denoted as omt1+, omt2+ and omt3+ after overlapping meiotic transcripts. omt1+ encodes a 12-kDa protein and omt2+ encodes a 11-kDa protein with homology to the bifunctional mammalian protein DCoH/PCBD. omt3+ does not have a significant open reading frame. The omt2+ transcript overlaps with both the omt1+ and omt3+ transcripts but the latter two transcripts do not overlap. omt1Delta and omt2Delta but not omt3Delta failed to form mature spore walls. The Omt1-GFP and Omt2-GFP fusion proteins localized to the outside and the inside of the spore walls, respectively. The sporulation-specific protein Meu10 and the spore wall components were abnormally localized in the spore walls of omt1Delta and omt2Delta.


CONCLUSIONS
The overlapping genes omt1+ and omt2+ express functional proteins that participate in spore wall maturation, indicating that gene overlap does not affect the physiological functions of the proteins encoded by these genes. Generation of overlapped RNA may be due to loose regulation of transcription termination during meiosis of S. pombe.
Kakihara
Yoshito
Y
Department of Molecular Genetics, Research Institute for Microbial Deseases, Osaka University, 3-1 Yamadaoka, Suita City, Osaka 565-0871, Japan.
Nabeshima
Kentaro
K
Hirata
Aiko
A
Nojima
Hiroshi
H
D002473:Cell Wall / Q000378:metabolism*; D005800:Genes, Fungal / Q000502:physiology*; D005807:Genes, Overlapping; D049452:Green Fluorescent Proteins; D006836:Hydro-Lyases / Q000737:chemistry; D008164:Luminescent Proteins / Q000378:metabolism; D008540:Meiosis; D016366:Open Reading Frames; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012568:Schizosaccharomyces / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics* / Q000378:metabolism; D013172:Spores, Fungal; D014158:Transcription, Genetic
D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D029702:Schizosaccharomyces pombe Proteins; D049452:Green Fluorescent Proteins; D006836:Hydro-Lyases; C076392:pterin-4a-carbinolamine dehydratase