Automated serum protein electrophoresis by Capillarys.
Capillary zone electrophoresis (CZE) of serum proteins is increasingly gaining impact in clinical laboratories. In this report, we evaluate automated capillary zone electrophoresis by Capillarys (Sebia, France). Within-run and between-run imprecision for the five electrophoretic fractions was <2% and <6%, respectively. Data obtained with Capillarys correlated with results obtained with agarose gel electrophoresis and Paragon CZE 2000 (Beckman Coulter, USA). Analysis of serum obtained from patients with inflammation, nephrotic syndrome, bisalbuminemia, and alpha1-antitrypsin deficiency revealed that Capillarys was able to detect these abnormalities. Two hundred thirty eight samples were analyzed by agarose gel electrophoresis, Capillarys, capillary electrophoresis using Paragon CZE 2000 system, and immunofixation. Sample selection was based on the presence of a disturbed morphology (e.g., spike) of the protein profile or hypogammaglobulinemia on agarose gel electrophoresis and/or Capillarys. Immunofixation revealed the presence of a monoclonal protein, oligoclonal bands, polyclonal pattern, and a normal profile in, respectively, 89, 66, 19, and 64 samples. With Capillarys, Paragon, and agarose gel electrophoresis, a spike and/or disturbed morphology of the profile was found in 222, 182, and 180 samples, respectively. In these samples, immunofixation was negative in 73 (33%), 46 (25%), and 39 (22%) samples, respectively. These data indicate that Capillarys has a lower specificity than agarose gel electrophoresis and Paragon 2000. Of the 89 samples with a monoclonal protein, Capillarys, Paragon, and agarose gel electrophoresis failed to detect, respectively, three, three, and one monoclonal protein(s). Interferences by radio-opaque agents, complement degradation products, fibrinogen, and triglycerides are described. In conclusion, automated capillary zone electrophoresis with Capillarys provides for reproducible, rapid, and reliable serum electrophoresis.
Bossuyt
Xavier
X
Laboratory Medicine, Immunology, University Hospital Leuven, Leuven, Belgium. xavier.bossuyt@uz.kuleuven.ac.be
Lissoir
Bénédicte
B
Mariën
Godelieve
G
Maisin
Diane
D
Vunckx
Jozef
J
Blanckaert
Norbert
N
Wallemacq
Pierre
P
D000328:Adult; D001774:Blood Chemical Analysis / Q000295:instrumentation* / Q000379:methods; D001798:Blood Proteins / Q000032:analysis*; D004587:Electrophoresis, Agar Gel / Q000295:instrumentation; D019075:Electrophoresis, Capillary / Q000295:instrumentation; D005260:Female; D006801:Humans; D008297:Male; D015203:Reproducibility of Results
D001798:Blood Proteins