Analysis of high mass peptides using a novel matrix-assisted laser desorption/ionisation quadrupole ion trap time-of-flight mass spectrometer.
A novel matrix-assisted laser desorption/ionisation quadrupole ion trap time-of-flight (MALDI QIT ToF) mass spectrometer has been used to analyse high mass peptide ions exceeding 2000 Da. Human adrenocorticotropic hormone (fragment 18-39) and oxidised bovine insulin chain B were utilised to evaluate the performance of the instrument both in MS and in MS/MS mode. Its ability to efficiently isolate ions and to fragment them using collisionally activated decomposition (CAD) has been demonstrated using mixtures diluted to the low-femtomole level on target. Additionally, multiple stage mass spectrometry (MS/MS/MS) provides a second-generation product ion spectrum in which new fragment ions are detected and new stretches of amino acids are identified.
Martin
Rachel L
RL
Shimadzu Biotech, Wharfside, Trafford Wharf Road, Manchester M17 1GP, UK.
Brancia
Francesco L
FL
Shimadzu Research Laboratory, Wharfside, Trafford Wharf Road, Manchester M17 1GP, UK.
D000324:Adrenocorticotropic Hormone / Q000032:analysis / Q000737:chemistry; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D006801:Humans; D007328:Insulin / Q000032:analysis / Q000737:chemistry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis* / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation* / Q000379:methods*
D007328:Insulin; D010446:Peptide Fragments; D000324:Adrenocorticotropic Hormone