title: Bacteroides forsythus in primary endodontic infections as detected by nested PCR.
abstract: The purpose of this study was to investigate the prevalence of Bacteroides forsythus in primary endodontic infections using a species-specific nested polymerase chain reaction assay. Samples were collected from 50 teeth having carious lesions, necrotic pulps, and different forms of periradicular diseases. DNA extracted from the samples was initially amplified using universal 16S rDNA primers. A second round of amplification used the first polymerase chain reaction products to detect a specific fragment of B. forsythus 16S rDNA. B. forsythus was detected in 13 of 22 asymptomatic cases (59.1%), 4 of 10 root canals associated with acute apical periodontitis (40%), and 9 of 18 cases diagnosed as acute periradicular abscesses (50%). There was no relationship between the presence of B. forsythus and the occurrence of symptoms. In general, this bacterial species was detected in 26 of 50 samples of endodontic infections (52%). The findings of this study support the assertion that this bacterial species is associated with infections of endodontic origin and suggest that B. forsythus may be involved in the pathogenesis of different forms of periradicular lesions.
authors.affiliation: Department of Endodontics, Faculty of Dentistry, Estácio de Sá University, Rio de Janeiro, Brazil.
authors.forename: José F
authors.initials: JF
authors.lastname: Siqueira
authors.forename: Isabela N
authors.initials: IN
authors.lastname: Rôças
mesh_terms: D001439:Bacteroides / Q000145:classification* / Q000235:genetics; D001442:Bacteroides Infections / Q000175:diagnosis*; D016009:Chi-Square Distribution; D004269:DNA, Bacterial / Q000032:analysis; D003731:Dental Caries / Q000382:microbiology; D003786:Dental Pulp Cavity / Q000382:microbiology; D003790:Dental Pulp Necrosis / Q000382:microbiology*; D006801:Humans; D010482:Periapical Abscess / Q000382:microbiology; D010485:Periapical Periodontitis / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S