Dietary arachidonic acid suppresses bone turnover in contrast to low dosage exogenous prostaglandin E(2) that elevates bone formation in the piglet.
This study was designed to compare the effects of dietary arachidonic acid (AA) versus prostaglandin E(2) (PGE(2)) on bone cell metabolism and bone mass. Twenty-eight piglets from 7 litters were randomized to 1 of 4 treatments for 15 days: fatty acid supplemented formula (FA: 0.8% of total fatty acids as AA and 0.1% of total fatty acids as DHA)+PGE(2) injections (0.1mg/kg/day), FA+saline injections, standard formula (STD: n-6:n-3 of 8:1) + PGE(2) injections or STD+saline injections. PGE(2) resulted in elevated osteoblast activity as indicated by plasma osteocalcin and also reduced urinary calcium excretion. Dietary FA resulted in reduced bone resorption as indicated by urinary N-telopeptide and reduced bone PGE(2). Both PGE(2) and FA treatments independently lead to elevated femur mineral content, but the combined treatment caused a reduction. Thus the mechanisms by which PGE(2) and FA lead to enhanced bone mass are distinct.
Lucia
Vienna D
VD
Department of Human Nutritional Sciences, University of Manitoba, H513 Duff Roblin Building, Winnipeg, Manitoba, Canada R3T 2N2.
Fitzpatrick-Wong
Shirley C
SC
Weiler
Hope A
HA
D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology*; D001842:Bone and Bones / Q000187:drug effects* / Q000378:metabolism*; D004032:Diet; D004041:Dietary Fats / Q000494:pharmacology; D005260:Female; D008297:Male; D010012:Osteogenesis / Q000187:drug effects*; D011458:Prostaglandins E / Q000494:pharmacology*; D013552:Swine
D004041:Dietary Fats; D011458:Prostaglandins E; D016718:Arachidonic Acid