title: A hedgehog-responsive region in the Drosophila wing disc is defined by debra-mediated ubiquitination and lysosomal degradation of Ci.
abstract: Transcription factor Ci mediates Hedgehog (Hh) signaling to determine the anterior/posterior (A/P) compartment of Drosophila wing disc. While Hh-inducible genes are expressed in A compartment cells abutting the A/P border, it is unclear how the boundaries of this region are established. Here, we have identified a Ci binding protein, Debra, that is expressed at relatively high levels in the band abutting the border of the Hh-responsive A compartment region. Debra mediates the polyubiquitination of full-length Ci, which then leads to its lysosomal degradation. Debra is localized in the multivesicular body, suggesting that the polyubiquitination of Ci directs its sorting into lysosome. Thus, Debra defines the border of the Hh-responsive region in the A compartment by inducing the lysosomal degradation of Ci.
authors.affiliation: Laboratory of Molecular Genetics, RIKEN Tsukuba Institute, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan.
authors.forename: Ping
authors.initials: P
authors.lastname: Dai
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Akimaru
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Ishii
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases; D004268:DNA-Binding Proteins / Q000378:metabolism*; D015536:Down-Regulation; D004330:Drosophila / Q000196:embryology* / Q000235:genetics; D029721:Drosophila Proteins; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000502:physiology*; D017353:Gene Deletion; D018507:Gene Expression Regulation, Developmental; D053823:Hedgehog Proteins; D008247:Lysosomes / Q000378:metabolism*; D008954:Models, Biological; D009024:Morphogenesis; D009154:Mutation; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D022161:Transport Vesicles / Q000378:metabolism; D014452:Ubiquitins / Q000502:physiology; D014921:Wings, Animal / Q000166:cytology / Q000196:embryology*
chemical_list: D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D053823:Hedgehog Proteins; D014157:Transcription Factors; D014452:Ubiquitins; C077245:hh protein, Drosophila; D017346:Protein Serine-Threonine Kinases; D017868:Cyclic AMP-Dependent Protein Kinases