title: Streptococcal diversity in oral biofilms with respect to salivary function.
abstract: OBJECTIVE
  In a previous study, we screened 149 subjects and established four groups high or low for salivary killing of oral bacteria, and for aggregation and live and dead adherence of oral bacteria (as a combined factor). Caries scores were significantly lower in both High Aggregation-Adherence groups. In this study we looked at the effects of those differences in salivary function on the quantity and diversity of oral biofilm streptococci.
  
  
  METHODS
  Subjects from those four groups were recalled for collection of overnight oral biofilm from buccal upper central incisors, lingual lower central incisors, buccal upper and lower first molars, and lingual upper and lower first molars. At each site, groups were compared for total biofilm (by DNA concentration), total streptococci (by quantitative PCR), and streptococcal diversity (by Streptococcus-specific denaturing gradient gel electrophoresis).
  
  
  RESULTS
  Total biofilm DNA and total streptococci were correlated. Both were highest on buccal molar surfaces and lowest on lingual lower central incisors, and both were significantly lower in the High Aggregation-Adherence groups (particularly at the buccal molar site). Fifty distinct bands were observed in denaturing gradient gels. There was great diversity within and between sites. Three major bands were present in almost every person at every site. Densities for two of those bands were significantly lower in both High Aggregation-Adherence groups. Other less-prevalent bands also showed the same pattern.
  
  
  CONCLUSIONS
  These findings are consistent with our caries results in suggesting that differences in salivary function can influence the quantity and composition of streptococci in oral biofilms.
authors.affiliation: Department of Oral Science, School of Dentistry, University of Minnesota, 17-252 Moos Tower, 515 Delaware Street SE, Minneapolis, MN 55455, USA. jrudney@tc.umn.edu
authors.forename: J D
authors.initials: JD
authors.lastname: Rudney
authors.forename: Y
authors.initials: Y
authors.lastname: Pan
authors.forename: R
authors.initials: R
authors.lastname: Chen
mesh_terms: D001422:Bacterial Adhesion; D015373:Bacterial Typing Techniques; D018441:Biofilms; D018533:Biomass; D004269:DNA, Bacterial / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006801:Humans; D007113:Immunity, Innate; D009055:Mouth / Q000276:immunology / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012463:Saliva / Q000276:immunology / Q000502:physiology*; D013223:Statistics as Topic; D013291:Streptococcus / Q000145:classification / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial