[Erythroid differentiation of K562 cells resistant to 2-(4'-dimethylaminostyryl)quinoline 1-oxide or 4-nitroquinoline 1-oxide is significantly increased after thymidine treatment].
We studied the effect of certain differentiation inducers-thymidine, sodium butyrate, and dimethyl sulfoxide--on the cells of parental line K562 and the derived sublines resistant to quinoline xenobiotics 2-(4-dimethylaminostyryl)quinoline 1-oxide or 4-nitroquinoline 1-oxide. The cells of the both derived sublines demonstrate cross-resistance to these xenobiotics, while the subline resistant to 2-(4-dimethylaminostyryl)quinoline 1-oxide is also resistant to ethidium bromide and colchicine. Treatment of cells of the sublines with thymidine but neither sodium butyrate nor dimethyl sulfoxide for 2 or 4 days considerably increases intracellular content of hemoglobin as compared to the parental cells K562. The revealed effect is due to increased hemoglobin content in the cells rather than to the increased proportion of hemoglobin-containing cells, which results from decreased proliferation rate observed in all cases.
Anisimov
A G
AG
Petrozavodsk State University, Prosp. Lenina 33, Petrozavodsk, 185640 Russia. anisimov@mainpgu.karelia.ru
Chekmasova
A A
AA
Volkova
T O
TO
Nemova
N N
NN
D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology*; D002087:Butyrates / Q000494:pharmacology; D002452:Cell Count; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003078:Colchicine / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D018432:Drug Resistance, Multiple; D006454:Hemoglobins / Q000096:biosynthesis; D006801:Humans; D020014:K562 Cells; D011804:Quinolines / Q000494:pharmacology*; D013343:Styrenes / Q000494:pharmacology*; D013936:Thymidine / Q000494:pharmacology*; D015262:Xenobiotics / Q000494:pharmacology*
C476556:2-(4'-dimethylaminostyryl)quinoline 1-oxide; D002087:Butyrates; D006454:Hemoglobins; D011804:Quinolines; D013343:Styrenes; D015262:Xenobiotics; D015112:4-Nitroquinoline-1-oxide; D003078:Colchicine; D013936:Thymidine; D004121:Dimethyl Sulfoxide