title: A chimeric mouse model established by allogenic in utero transplantation.
abstract: In utero stem cells transplantation is a promising approach to the prenatal treatment of diseases. In order to investigate the fate of the stem cells after in utero transplantation, we have established a chimeric mouse model with the method of in utero transplantation. Mononuclear cells (including stem cells/progenitor cells) derived from male mouse bone marrow were injected into fetal mouse peritoneal cavity during the pre-immune period. The donor cells in the circulatory blood of female recipients were identified by fluorescence in situ hybridization (FISH), and the Y-chromosome specific DNA was detected by PCR as well as real-time quantitative PCR after the recipient mice were born. The results showed that a total of 4 female recipient mice were chimeric in their peripheral blood. Significantly, the donor cells in three chimeric mice persisted up to six months.
authors.affiliation: Shanghai Institute of Medical Genetics, Shanghai Children's Hospital, Shanghai Communication University, Shanghai 200040, China.
authors.forename: Mo-Lin
authors.initials: ML
authors.lastname: Wang
authors.forename: Jing-Bin
authors.initials: JB
authors.lastname: Yan
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Xiao
authors.forename: Zhao-Rui
authors.initials: ZR
authors.lastname: Ren
authors.forename: Shu-Zhen
authors.initials: SZ
authors.lastname: Huang
authors.forename: Yi-Tao
authors.initials: YT
authors.lastname: Zeng
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D005260:Female; D005333:Fetus / Q000601:surgery*; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D051379:Mice; D023421:Models, Animal; D011247:Pregnancy; D011256:Pregnancy Outcome; D018183:Transplantation Chimera / Q000097:blood* / Q000235:genetics; D014184:Transplantation, Homologous; D014886:Weaning