Effects of UV dose on formation of spontaneously developing pocks in Streptomyces azureus ATCC14921.
Spontaneously developing pocks (S pocks) of Streptomyces azureus ATCC14921 were formed by the both functions of conjugative plasmid pSA1 and lysogenic phage SAt2. The formation was affected by the dose of UV irradiation. The mean pock diameter in cultures treated with UV light at 0, 7.1, 14.2 and 21.3 x 10(2) microW. erg/cm, respectively, were 1.3, 0.4, 2.2, and 0.5 mm. The dose affected conjugative plasmid pSA1 related to pock formation. There was UV damage of autonomous pSA1 replicon and UV induction of the chromosomal integrated sequence. Increases and decreases in the amount of autonomous pSA1 replicon corresponded to increases and decreases, respectively, in the diameter of the pocks. Both pSA1 and SAt2 syntheses were developed in the large pocks (1.3 and 2.2 mm), but only SAt2 synthesis was developed in the pinhole pocks (0.4 and 0.5 mm).
Yamada
Shuji
S
Microbial Genetics Division, Institute of Genetic Resources, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
Suenaga
Hikaru
H
Doi
Katsumi
K
Yoshino
Sadazo
S
Ogata
Seiya
S
D004307:Dose-Response Relationship, Radiation; D008854:Microscopy, Electron; D010957:Plasmids / Q000528:radiation effects*; D012093:Replicon / Q000528:radiation effects; D013171:Spores, Bacterial; D013302:Streptomyces / Q000235:genetics / Q000254:growth & development*; D014466:Ultraviolet Rays