[Change in spectral properties of nitrosohemoglobin induced by UV light].
The effect of UV radiation on the spectral characteristics of human nitrosohemoglobin in a wide dose range (151-4530 J/m2) was studied. It was shown that the irradiation of hemoprotein solutions with low doses of UV light (151-453 J/m2) led to local conformational rearrangements of the iron porphyrin moiety of the molecule. The apoprotein exhibited a high photostability under the conditions of the experiment. High doses of UV radiation (1359-4530 J/m2) induced the accumulation of methemoglobin in the protein sample. A scheme of the photoinduced formation of methemoglobin was elaborated.
Putintseva
O V
OV
Voronezh State University, Universitetskaya pl. 1, Voronezh, 394693 Russia.
Artiukhov
V G
VG
Kalaeva
E A
EA
D006454:Hemoglobins / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D009603:Nitroso Compounds / Q000737:chemistry*; D011487:Protein Conformation; D012996:Solutions; D014466:Ultraviolet Rays
D006454:Hemoglobins; D009603:Nitroso Compounds; D012996:Solutions