title: Proliferation of neural and neuronal progenitors after global brain ischemia in young adult macaque monkeys.
abstract: To investigate the effect of global cerebral ischemia on brain cell proliferation in young adult macaques, we infused 5-bromo-2'-deoxyuridine (BrdU), a DNA replication indicator, into monkeys subjected to ischemia or sham-operated. Subsequent quantification by BrdU immunohistochemistry revealed a significant postischemic increase in the number of BrdU-labeled cells in the hippocampal dentate gyrus, subventricular zone of the temporal horn of the lateral ventricle, and temporal neocortex. In all animals, 20-40% of the newly generated cells in the dentate gyrus and subventricular zone expressed the neural progenitor cell markers Musashi1 or Nestin. A few BrdU-positive cells in postischemic monkeys were double-stained for markers of neuronal progenitors (class III beta-tubulin, TUC4, doublecortin, or Hu), neurons (NeuN), or glia (S100beta or GFAP). Our results suggest that ischemia activates endogenous neuronal and glial precursors residing in diverse locations of the adult primate central nervous system.
authors.affiliation: Department of Neurosurgery, Division of Neuroscience, Kanazawa University Graduate School of Medical Science, Kanazawa, 920-8641, Japan.
authors.forename: Anton B
authors.initials: AB
authors.lastname: Tonchev
authors.forename: Tetsumori
authors.initials: T
authors.lastname: Yamashima
authors.forename: Liang
authors.initials: L
authors.lastname: Zhao
authors.forename: Hirotaka James
authors.initials: HJ
authors.lastname: Okano
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Okano
mesh_terms: D000818:Animals; D015415:Biomarkers; D002545:Brain Ischemia / Q000378:metabolism* / Q000503:physiopathology; D001973:Bromodeoxyuridine; D016923:Cell Death / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D004249:DNA Damage / Q000502:physiology; D018891:Dentate Gyrus / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D005260:Female; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000378:metabolism; D020547:Lateral Ventricles / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D008251:Macaca; D009416:Nerve Regeneration / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D009473:Neuronal Plasticity / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D010641:Phenotype; D016601:RNA-Binding Proteins / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D013687:Telencephalon / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D013702:Temporal Lobe / Q000166:cytology / Q000254:growth & development / Q000378:metabolism
chemical_list: D015415:Biomarkers; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; D064231:Nestin; D016601:RNA-Binding Proteins; D001973:Bromodeoxyuridine