title: Autologous human keratinocytes cultured on membranes composed of benzyl ester of hyaluronic acid for grafting in nonhealing diabetic foot lesions: a pilot study.
abstract: Diabetic foot complications are the most common cause of nontraumatic lower extremity amputations in the industrialised world. Unsatisfactory healing requires advanced therapeutic strategies, such as the use of skin grafts, which may represent a helpful option for wound coverage. Alternatively, a method using autologous keratinocytes grown to thin sheet grafts is available. The purpose of this pilot study was to investigate the application of autologous human keratinocytes cultured on membranes composed of benzyl ester of hyaluronic acid (Laserskin autograft) to diabetic foot ulcers. We studied 14 patients with type 2 diabetes mellitus and a nonhealing diabetic foot lesion, defined as existing longer than 6 months or with no wound healing apparent for 12 weeks. Between 7 and 64 days after the transplantation (depending on the size of the ulceration), 11/14 of the lesions were completely healed. The transplantation of autologous keratinocytes may allow faster closure of diabetic foot lesions and subsequently reduce length of hospitalization. This method can easily be planned with regard to logistics and time, and furthermore, this therapy option can be carried out by the diabetologist.
authors.affiliation: Department of Endocrinology and Metabolism, University Medical School of Magdeburg, Magdeburg, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Lobmann
authors.forename: Daniel
authors.initials: D
authors.lastname: Pittasch
authors.forename: Isabel
authors.initials: I
authors.lastname: Mühlen
authors.forename: Hendrik
authors.initials: H
authors.lastname: Lehnert
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002478:Cells, Cultured; D017719:Diabetic Foot / Q000473:pathology / Q000503:physiopathology* / Q000601:surgery*; D005260:Female; D006801:Humans; D006820:Hyaluronic Acid / Q000031:analogs & derivatives*; D015603:Keratinocytes / Q000473:pathology* / Q000637:transplantation*; D008297:Male; D008567:Membranes, Artificial; D008875:Middle Aged; D010865:Pilot Projects; D014182:Transplantation, Autologous; D014945:Wound Healing
chemical_list: D008567:Membranes, Artificial; C086358:hyaluronic acid benzyl ester; D006820:Hyaluronic Acid