[The effect of losing glycosylation sites near the receptor-binding region on the receptor phenotype of the human influenza virus H1N1].
The receptor properties of influenza virus (IF) isolates/SSSR/90/77 are studied. The isolates are peculiar for losing glycosylation sites (GS) at the Asn131 receptor-binding region (GS131) after passaging in mice and at the Asn158 region (GS158) after cultivation in the presence of mouse serum. The loss of each carbohydrate residue increases the influenza virus affinity for carbohydrate chains with the terminal group Neu5Ac alpha 2-6Gal and reduces its affinity for Neu5Ac alpha 2-3Gal receptors. The effect is more pronounced in the GS158-depleted virus. Upon substitution of asparagine by aspartic acid, the electrostatic component of virus binding to the receptor is altered because of the increased negative charge on hemagglutinin. The virus receptor phenotype changes depending on the cultivation conditions. The isolate adapted to mice has higher affinity to mouse lung cell receptors, while the virus propagated in chick embryos in the presence of inhibitors has higher affinity to allantoic membrane cells.
Marinina
V P
VP
Tchumakov Institute of Poliomyelitis and Viral Encephalitides, Russian Academy of Sciences, Moscow, 142782 Russia.
Gambarian
A S
AS
Bovin
N V
NV
Tuzikov
A B
AB
Shilov
A A
AA
Sinitsyn
B V
BV
Matrosovich
M N
MN
D000818:Animals; D001216:Asparagine / Q000378:metabolism; D001665:Binding Sites; D050260:Carbohydrate Metabolism; D002462:Cell Membrane / Q000821:virology; D002642:Chick Embryo / Q000821:virology; D006031:Glycosylation; D006388:Hemagglutinins / Q000378:metabolism; D053118:Influenza A Virus, H1N1 Subtype; D009980:Influenza A virus / Q000472:pathogenicity / Q000502:physiology*; D008168:Lung / Q000378:metabolism / Q000821:virology; D051379:Mice; D009154:Mutation; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D010641:Phenotype; D011956:Receptors, Cell Surface / Q000378:metabolism*; D055672:Static Electricity
D006388:Hemagglutinins; D011956:Receptors, Cell Surface; D001216:Asparagine; D019158:N-Acetylneuraminic Acid