title: Field survey of Glycyrrhiza plants in Central Asia (1). Characterization of G. uralensis, G. glabra and the putative intermediate collected in Kazakhstan.
abstract: The characteristics of Glycyrrhiza plants from 12 collection sites in southeastern Kazakhstan were investigated. G. uralensis was observed at 9 of the sites from Almaty to Shu, and G. glabra was observed at 8 sites. At 4 sites near Shu, and 1 site near Almaty, G. glabra and G. uralensis grew together forming a mixed population, and intermediate-type plants between them were also observed at 3 sites. Although two nucleotide substitutions of the chloroplast rbcL gene were observed between G. uralensis and G. glabra, rbcL sequences of the intermediate-types were divided into G. uralensis-type (G-A type) and G. glabra-type (A-T type). HPLC analysis of the roots indicated that species-specific flavonoids, glabridin and glycycoumarin, were detected in the roots of G. glabra and G. uralensis, respectively, but neither flavonoid was detected in underground parts of the intermediate-types. HPLC analysis of their leaves indicated a significant difference among G. uralensis, G. glabra and the intermediate-type plants. Both G. glabra-specific and G. uralensis-specific compounds were detected in the leaves of the intermediate-type, thus suggesting that the intermediate plants are hybrids of G. glabra and G. uralensis.
authors.affiliation: Gifu Pharmaceutical University, 5-6-1 Mitahora-higashi, Gifu 502-8585, Japan. hayashih@gifu-pu.ac.jp
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Hayashi
authors.forename: Sayaka
authors.initials: S
authors.lastname: Hattori
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Inoue
authors.forename: Kanat
authors.initials: K
authors.lastname: Sarsenbaev
authors.forename: Michiho
authors.initials: M
authors.lastname: Ito
authors.forename: Gisho
authors.initials: G
authors.lastname: Honda
mesh_terms: D002851:Chromatography, High Pressure Liquid; D017343:Genes, Plant / Q000235:genetics; D006035:Glycyrrhiza / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D007623:Kazakhstan; D018517:Plant Roots / Q000737:chemistry; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics*; D013045:Species Specificity
chemical_list: C101625:RbcL protein, plastid; D012273:Ribulose-Bisphosphate Carboxylase