title: Novel proangiogenic effect of factor XIII associated with suppression of thrombospondin 1 expression.
abstract: OBJECTIVE
  Factor XIII (FXIII), a plasma transglutaminase that stabilizes fibrin clots at the final stages of blood coagulation by crosslinking fibrin monomers, is essential for embryo implantation and participates in tissue remodeling and wound healing, processes that involve angiogenesis. The aim of our study was to analyze the effect of FXIII on angiogenesis using in vitro and in vivo models and to examine the role of FXIII in the basic steps of angiogenesis, ie, migration, proliferation, and apoptosis/cell survival.
  
  
  RESULTS
  In the Matrigel tube formation model, only FXIIIa caused a dose-dependent enhancement of array formation. This proangiogenic effect was not associated with alterations in vascular endothelial growth factor (VEGF) protein levels nor VEGF or VEGFR2 mRNA levels. FXIIIa, but not nonactivated or transglutaminase-inactivated FXIII, significantly enhanced endothelial cell migration and proliferation and inhibited apoptosis. After treatment of HUVECs with FXIIIa, almost complete disappearance of mRNA of thrombospondin 1 (TSP-1) and a marked reduction in the secretion of TSP-1 protein were observed. A reduction in TSP-1 protein synthesis, although to a lesser extent, was observed on treatment of microvascular endothelial cells with FXIIIa. In a rabbit cornea model, injection of FXIIIa caused neovascularization associated with almost complete disappearance of TSP-1 in the cornea.
  
  
  CONCLUSIONS
  These results show that FXIIIa exhibits a novel proangiogenic activity that is associated with downregulation of TSP-1 and also involves stimulation of endothelial cell proliferation and migration and inhibition of apoptosis. These findings might shed light on the mechanism by which FXIII mediates tissue repair and remodeling.
authors.affiliation: Institute of Thrombosis and Hemostasis, Sheba Medical Center, Tel Hashomer, Israel.
authors.forename: Rima
authors.initials: R
authors.lastname: Dardik
authors.forename: Arieh
authors.initials: A
authors.lastname: Solomon
authors.forename: Joseph
authors.initials: J
authors.lastname: Loscalzo
authors.forename: Regina
authors.initials: R
authors.lastname: Eskaraev
authors.forename: Ann
authors.initials: A
authors.lastname: Bialik
authors.forename: Iris
authors.initials: I
authors.lastname: Goldberg
authors.forename: Ginette
authors.initials: G
authors.lastname: Schiby
authors.forename: Aida
authors.initials: A
authors.lastname: Inbal
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D003315:Cornea / Q000098:blood supply; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005176:Factor XIII / Q000378:metabolism* / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects / Q000502:physiology; D006801:Humans; D018919:Neovascularization, Physiologic / Q000187:drug effects / Q000502:physiology*; D011817:Rabbits; D019700:Thrombospondin 1 / Q000096:biosynthesis / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D019700:Thrombospondin 1; D042461:Vascular Endothelial Growth Factor A; D005176:Factor XIII