Determination of inorganic phosphate in serum and saliva using a synthetic receptor.
[structure: see text] A C(3)(v) symmetric synthetic receptor (1) was employed in an indicator-displacement assay to determine the phosphate concentrations in both horse serum and human saliva at biological pH. The determination of the phosphate concentrations in the serum and saliva using the colorimetric assay were 1.6 and 5.1 mM, respectively. These results further accentuate the usefulness of synthetic receptors in truly practical applications.
Tobey
Suzanne L
SL
Department of Chemistry and Biochemistry, University of Texas, Austin, Texas 78712, USA.
Anslyn
Eric V
EV
D000818:Animals; D003124:Colorimetry / Q000379:methods; D003300:Copper / Q000737:chemistry*; D005452:Fluoresceins / Q000737:chemistry; D006736:Horses; D006801:Humans; D007700:Kinetics; D009942:Organometallic Compounds / Q000737:chemistry*; D010710:Phosphates / Q000032:analysis* / Q000097:blood*; D012463:Saliva / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D005452:Fluoresceins; D009942:Organometallic Compounds; D010710:Phosphates; C024098:6-carboxyfluorescein; D003300:Copper