title: Fosfomycin inhibits NF-kappaB activation in U-937 and Jurkat cells.
abstract: Fosfomycin exerts anti-inflammatory effects through inhibiting the production of proinflammatory cytokines. Transcription of the genes for these proinflammatory cytokines is regulated by NF-kappaB. We tested the hypothesis that fosfomycin inhibits the activation of NF-kappaB induced by tumor necrosis factor-alpha (TNF-alpha) in human monocytic U-937 cells, and a T cell line (Jurkat). Western blot analysis demonstrated that fosfomycin inhibits NF-kappaB activation in both cells. Flow cytometry revealed that fosfomycin suppresses NF-kappaB activation in both cells in a dose-related manner. These findings are consistent with the idea that fosfomycin suppresses the production of proinflammatory cytokines via inhibition of NF-kappaB activation.
authors.affiliation: Department of Pediatrics, Yamaguchi University School of Medicine, 1-1-1 Minamikogushi, Yamaguchi 755-8505, Ube, Japan.
authors.forename: Yasuto
authors.initials: Y
authors.lastname: Yoneshima
authors.forename: Takashi
authors.initials: T
authors.lastname: Ichiyama
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ayukawa
authors.forename: Tomoyo
authors.initials: T
authors.lastname: Matsubara
authors.forename: Susumu
authors.initials: S
authors.lastname: Furukawa
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015153:Blotting, Western; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D005578:Fosfomycin / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D019169:Jurkat Cells; D016328:NF-kappa B / Q000235:genetics* / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D020298:U937 Cells
chemical_list: D000900:Anti-Bacterial Agents; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D005578:Fosfomycin