NMR experiments for the measurement of proton-proton and carbon-carbon residual dipolar couplings in uniformly labelled oligosaccharides.
A 2D-HSQC-carbon selective/proton selective-constant time COSY, 2D-HSQC-(sel C, sel H)-CT COSY experiment, which is applicable to uniformly (13)C isotopically enriched samples (U-(13)C) of oligosaccharides or oligonucleotides is proposed for the measurement of proton-proton RDC in crowded regions of 2D-spectra. In addition, a heteronuclear constant time-COSY experiment, (13)C-(13)C CT-COSY, is proposed for the measurement of one bond carbon-carbon RDC in these molecules. These two methods provide an extension, to U-(13)C molecules, of the original homonuclear constant time-COSY experiment proposed by Tian et al. (1999) for saccharides. The combination of a number of these RDC with NOE data may provide the method of choice to study oligosaccharide conformation in the free and receptor-bound state.
Martin-Pastor
Manuel
M
Laboratorio Integral de Estructura de Biomoléculas Jose. R. Carracido, Unidade de Resonancia Magnética, RIAIDT, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain. mmartin@usc.es
Canales-Mayordomo
Angeles
A
Jiménez-Barbero
Jesús
J
D002236:Carbohydrate Conformation; D002244:Carbon / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D009844:Oligosaccharides / Q000737:chemistry*; D011522:Protons
D009844:Oligosaccharides; D011522:Protons; D002244:Carbon