title: Bonded interface between a self-curing resin and dentin primed with a metalloprotein.
abstract: The purpose of this study was to investigate adhesive bonding between a self-curing luting agent and dentin conditioned with a metalloprotein in terms of resin-dentin hybridization and interfacial polymerization. Of the six experimental primers containing bovine heart cytochrome c (BHCC), three contained 2-hydroxyethylmethacrylate (HEMA) and the remaining three did not. The self-curing luting agent used consisted of methyl methacrylate (MMA) and tri-n-butylborane (TBB) with or without 4-methacryloyloxyethyl trimellitate anhydride (4-META). Bovine dentin surfaces were etched with 10wt% phosphoric acid, primed, and then bonded with stainless steel rods. After 24 h of water storage, the optimum bond strength was obtained with the 4-META/MMA-TBB luting agent and the aqueous primer contained 0.1 micromol/g BHCC and 35wt% HEMA. Microscopic observations showed continuity among the luting agent, the hybridized dentin, and the dentin substrate. A model experiment suggested that BHCC accelerates the polymerization of the 4-META/MMA/HEMA mixture from the hybridized dentin.
authors.affiliation: Department of Developmental and Reconstructive Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan. yohsuke@net.nagasaki-u.ac.jp
authors.forename: Yohsuke
authors.initials: Y
authors.lastname: Taira
authors.forename: Hideo
authors.initials: H
authors.lastname: Matsumura
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Atsuta
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000818:Animals; D001896:Boron Compounds / Q000737:chemistry; D002417:Cattle; D003574:Cytochrome c Group / Q000737:chemistry; D003804:Dentin / Q000737:chemistry; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D066298:In Vitro Techniques; D008422:Materials Testing; D008667:Metalloproteins / Q000737:chemistry*; D020366:Methylmethacrylate / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D019279:Resin Cements / Q000737:chemistry*; D013718:Tensile Strength
chemical_list: D000180:Acrylic Resins; D001896:Boron Compounds; D003574:Cytochrome c Group; D017220:Dentin-Bonding Agents; D008667:Metalloproteins; D019279:Resin Cements; C009523:tri-n-butylborane; D020366:Methylmethacrylate; C031172:4-META resin