Standardisation and establishment of a rabies ELISA test in European laboratories for assessing the efficacy of oral fox vaccination campaigns.
A simple and rapid enzyme linked immunosorbent assay (ELISA) to detect rabies antibodies in field fox sera has been standardised and established in several European laboratories. The same panels of 100 coded sera were investigated by four laboratories using this ELISA assay and reference serum neutralisation techniques (fluorescent antibody virus neutralisation (FAVN) test and rapid fluorescent focus inhibition test (RFFIT)). This indirect ELISA technique is highly correlated with conventional seroneutralisation test on cell culture. Results obtained with this ELISA test provide an almost perfect agreement between laboratories while the agreement of FAVN test and RFFIT results is substantial and very satisfactory. In view of this study, this simple and rapid ELISA test is a suitable tool for evaluating the sero-conversion rate in fox populations following oral rabies vaccination campaigns in European countries.
Cliquet
Florence
F
AFSSA Nancy, Laboratoire d'études et de recherches sur la rage et la pathologie des animaux sauvages, B.P. 9, 54220 Malzeville, France. f.cliquet@afssa.fr
Müller
Thomas
T
Mutinelli
Franco
F
Geronutti
Sabrina
S
Brochier
Bernard
B
Selhorst
Thomas
T
Schereffer
Jean-Luc
JL
Krafft
Nicolas
N
Burow
Jeanette
J
Schameitat
Astrid
A
Schlüter
Hartmut
H
Aubert
Michel
M
D000284:Administration, Oral; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards; D005060:Europe; D005455:Fluorescent Antibody Technique; D005589:Foxes / Q000276:immunology*; D007753:Laboratories / Q000592:standards*; D009500:Neutralization Tests; D011818:Rabies / Q000276:immunology / Q000517:prevention & control* / Q000662:veterinary*; D011819:Rabies Vaccines / Q000008:administration & dosage / Q000276:immunology*; D012015:Reference Standards; D012680:Sensitivity and Specificity; D014611:Vaccination / Q000662:veterinary
D000914:Antibodies, Viral; D011819:Rabies Vaccines