OptiPrep density gradient solutions for macromolecules and macromolecular complexes.
Any density gradient for the isolation of mammalian cells should ideally only expose the sedimenting particles to an increasing concentration of the gradient solute. Thus they will experience only an increasing density and viscosity, other parameters such as osmolality, pH, ionic strength and the concentration of important additives (such as EDTA or divalent cations) should remain as close to constant as possible. This Protocol Article describes the strategies for the dilution of OptiPrep in order to prepare such solutions for mammalian cells.
Graham
John M
JM
School of Biomolecular Sciences, Liverpool John Moores University. john@jgrescon.fsbusiness.co.uk
D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D002499:Centrifugation, Density Gradient / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D046911:Macromolecular Substances; D009696:Nucleic Acids / Q000302:isolation & purification; D009994:Osmolar Concentration; D011506:Proteins / Q000302:isolation & purification; D012996:Solutions / Q000737:chemistry; D014283:Triiodobenzoic Acids / Q000737:chemistry; D014771:Virion / Q000302:isolation & purification
D046911:Macromolecular Substances; D009696:Nucleic Acids; D011506:Proteins; D012996:Solutions; D014283:Triiodobenzoic Acids; C044834:iodixanol