title: Structural and functional analysis of a lycopene beta-monocyclase gene isolated from a unique marine bacterium that produces myxol.
abstract: A gene coding for lycopene beta-monocyclase, which metabolizes lycopene (psi,psi-carotene) to gamma-carotene (beta,psi-carotene), was isolated for the first time from a unique marine bacterium strain P99-3 that produces myxol (a gamma-carotene derivative). This lycopene beta-monocyclase gene (designated crtYm) was included in the gene cluster which contained carotenoid biosynthetic gene (crtI, crtB, crtZ, crtY, and crtA) homologs. CrtYm, the CrtY homolog, metabolized lycopene to gamma-carotene, which was confirmed by deletion/expression analysis of the crtYm and by subsequent analysis of the metabolites from lycopene based on the retention times on high-performance liquid chromatography, UV-visible absorption spectra, and mass spectrometry.
authors.affiliation: Marine Biotechnology Institute, 3-75-1 Heita, Kamaishi-shi, Iwate 026-0001, Japan. maki.teramoto@mbio.jp
authors.forename: Maki
authors.initials: M
authors.lastname: Teramoto
authors.forename: Shinichi
authors.initials: S
authors.lastname: Takaichi
authors.forename: Yukie
authors.initials: Y
authors.lastname: Inomata
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ikenaga
authors.forename: Norihiko
authors.initials: N
authors.lastname: Misawa
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002338:Carotenoids / Q000096:biosynthesis*; D005417:Flavobacterium / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D019753:Intramolecular Lyases / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D002338:Carotenoids; D019753:Intramolecular Lyases; C070925:lycopene cyclase-isomerase