Developmental expression of XEEL, a novel molecule of the Xenopus oocyte cortical granule lectin family.
We have isolated cDNA clones from a Xenopus laevis embryo library that encode a predicted translation product of 342 amino acids containing a signal sequence for secretion. The predicted protein has 62-70% amino acid identity with the Xenopus oocyte cortical granule lectin (XCGL), the mouse intelectin, the human HL-1/intelectin and HL-2. Onset of gene expression occurs by gastrulation, and the transcripts localize in non-ciliated epidermal cells all over the tailbud embryos. The results suggest that the molecule, designated XEEL ( Xenopus embryonic epidermal lectin), is a novel XCGL family molecule secreted from the embryonic epidermis.
Nagata
Saburo
S
Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, Mejirodai 2-8-1, Bunkyoku, 112-8681 Tokyo, Japan. s-nagata@fc.jwu.ac.jp
Nakanishi
Misato
M
Nanba
Reiko
R
Fujita
Naoko
N
D000818:Animals; D001483:Base Sequence; D000078404:Epidermal Cells; D004817:Epidermis / Q000196:embryology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D017403:In Situ Hybridization; D037181:Lectins, C-Type / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family; D009865:Oocytes / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014982:Xenopus laevis / Q000196:embryology*
D037181:Lectins, C-Type