title: Potential limitations in using minor histocompatibility antigen-specific cytotoxic T cells for targeting solid tumor cells.
abstract: We have shown previously that KIAA0223, a gene encoding a minor histocompatibility antigen, HA-1, whose expression was believed to be restricted to the hematopoietic cells, is aberrantly expressed in some solid tumor cell lines. However, its significance in tumor immunity needs to be determined. Cytotoxic activity of HA-1(H)-specific cytotoxic T lymphocytes (CTLs) was assessed against solid tumor cell lines expressing KIAA0223 using (51)Cr release assays. Five of seven cell lines were lysed when HLA-A*0201 was adequately expressed. One of the two CTL-resistant cell lines became susceptible after treatment with IFN-gamma and TNF-alpha, while the other was lysed only after pulsing with HA-1(H) peptide. In most cell lines tested, HA-1(H) peptide was properly generated and presented for recognition by the CTL. However, impaired antigen processing and presentation observed in this study may result in escape from CTL recognition in vivo, as well as in vitro, as observed in this study.
authors.affiliation: Division of Immunology, Aichi Cancer Center Research Institute, Nagoya, Japan.
authors.forename: Mikinori
authors.initials: M
authors.lastname: Miyazaki
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Akatsuka
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Nishida
authors.forename: Nobuharu
authors.initials: N
authors.lastname: Fujii
authors.forename: Akio
authors.initials: A
authors.lastname: Hiraki
authors.forename: Kazuma
authors.initials: K
authors.lastname: Ikeda
authors.forename: Kunio
authors.initials: K
authors.lastname: Tsujimura
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Kuzushima
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Morishima
authors.forename: Shigeki
authors.initials: S
authors.lastname: Sato
authors.forename: Ryuzo
authors.initials: R
authors.lastname: Ueda
authors.forename: Toshitada
authors.initials: T
authors.lastname: Takahashi
mesh_terms: D015972:Gene Expression Regulation, Neoplastic; D015234:HLA-A Antigens / Q000235:genetics / Q000276:immunology; D015789:HLA-A2 Antigen / Q000235:genetics / Q000378:metabolism; D006801:Humans; D015778:Minor Histocompatibility Antigens / Q000276:immunology*; D009369:Neoplasms / Q000235:genetics / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014407:Tumor Cells, Cultured
chemical_list: D015234:HLA-A Antigens; C435939:HLA-A*02:01 antigen; D015789:HLA-A2 Antigen; D015778:Minor Histocompatibility Antigens