Modulatory role of IL10 in endothelial cell damage and platelet adhesion.
This study explores the possibility of a regulatory role for cytokine IL-10 in platelet aggregation as an active vascular repair mechanism. Endothelial cells from human umbilical cord vein were cultured in the presence of different IL-10 concentrations (0-100 ng/ml). Platelet-rich plasma was then added to these cultures and allowed to act for 30 minutes. To rule out blood plasma involvement, washed platelets were also incubated with IL-10 (0-100 ng/ml). Changes in endothelial cell morphology were observed depending on the IL-10 concentration used; apoptotic cells appearing at the highest IL-10 concentration. Greatest platelet adhesion was noted at the highest IL-10 concentration. It was concluded that, in this in vitro model, low IL-10 levels do not affect cell viability or the pattern of platelet adhesion, but at high doses, this cytokine induces cell death and enhances platelet deposition.
Gimeno
M J
MJ
Department of Morphological Sciences and Surgery, Faculty of Medicine, University of Alcalá, Alcalá de Henares, Madrid, Spain.
Pascual
G
G
García-Honduvilla
N
N
Prieto
A
A
Alvarez de Mon
M
M
Bellón
J M
JM
Buján
J
J
D017209:Apoptosis; D001792:Blood Platelets / Q000378:metabolism; D016923:Cell Death; D002455:Cell Division; D002470:Cell Survival; D002478:Cells, Cultured; D053938:DNA Fragmentation; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000378:metabolism* / Q000473:pathology*; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D020287:In Situ Nick-End Labeling; D016753:Interleukin-10 / Q000494:pharmacology / Q000502:physiology*; D008855:Microscopy, Electron, Scanning; D010949:Plasma / Q000378:metabolism; D010973:Platelet Adhesiveness; D013997:Time Factors; D014471:Umbilical Veins / Q000166:cytology*
D016753:Interleukin-10