Differential effect of NaCl and polyethylene glycol on the ultrastructure of chloroplasts in rice seedlings.
Ionic and osmotic effects of salinity on the ultrastructure of chloroplasts in salt-treated rice seedlings were investigated. After rice seedlings were grown in hydroponic culture for three weeks, they were treated with NaCl and polyethylene glycol (PEG) 4000 both at a water potential of -1.0 MPa for 3 days. The most notable difference in ultrastructural change between NaCl and PEG treatment was observed in the damage in chloroplast membranes. NaCl induced swelling of thylakoids and caused only a slight destruction of the chloroplast envelope. PEG caused severe destruction of the chloroplast envelope compared with NaCl, however thylakoids did not swell. Our observations suggested that in salt-treated rice plants, the ionic effects induced swelling of thylakoids and the osmotic effects caused the destruction of chloroplast envelope.
Yamane
Koji
K
Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan.
Kawasaki
Michio
M
Taniguchi
Mitsutaka
M
Miyake
Hiroshi
H
D002736:Chloroplasts / Q000187:drug effects* / Q000648:ultrastructure*; D007425:Intracellular Membranes / Q000187:drug effects / Q000648:ultrastructure; D012275:Oryza / Q000166:cytology* / Q000187:drug effects* / Q000648:ultrastructure; D011092:Polyethylene Glycols / Q000494:pharmacology*; D036226:Seedlings / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D012965:Sodium Chloride / Q000494:pharmacology*; D020524:Thylakoids / Q000187:drug effects / Q000648:ultrastructure
D011092:Polyethylene Glycols; D012965:Sodium Chloride