Simultaneous determination of ketamine and xylazine in canine plasma by liquid chromatography with ultraviolet absorbance detection.
An isocratic reversed-phase high-performance liquid chromatographic method for the simultaneous determination of ketamine and xylazine in canine plasma is described. Plasma samples (500 microl) are cleaned up via liquid-liquid extraction. The analytes and the internal standard clonidine are separated on a cyano (CN) column using a mobile phase containing acetonitrile-0.005 M phosphate buffer adjusted to pH 5.5 (3:2) at a detection wavelength of 215 nm. The method was validated according to specificity, sensitivity, accuracy and reproducibility and was used to determine the plasma concentrations of both compounds in dogs after intramuscular injection.
Niedorf
Frank
F
Institute for Pharmacology, Toxicology and Pharmacy, School of Veterinary Medicine Hannover, Bünteweg 17, 30559 Hannover, Germany. frank.niedorf@tiho-hannover.de
Bohr
Hans-Herbert
HH
Kietzmann
Manfred
M
D000316:Adrenergic alpha-Agonists / Q000097:blood*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004285:Dogs; D018691:Excitatory Amino Acid Antagonists / Q000097:blood*; D007649:Ketamine / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D014991:Xylazine / Q000097:blood*
D000316:Adrenergic alpha-Agonists; D018691:Excitatory Amino Acid Antagonists; D014991:Xylazine; D007649:Ketamine