Capturing ion exchanger-bound infectious pancreatic necrosis virus--design and application for large volume water samples.
A method was designed for rapid and reliable demonstration of the presence of infectious pancreatic necrosis virus retrieved from 5 l water samples. Viruses together with an added carrier protein were adsorbed to a resin of an added anion exchanger. Then the resin was collected rapidly and quantitatively through a specific device which we designed. The resin was transferred to a column from which the viruses were eluted, and subsequently further concentrated by acid precipitation, followed by SDS-polyacrylamide gel electrophoresis and Western blot analysis. Serological results were obtained within 24 h after the water sample was introduced into the laboratory. Proteins were recovered at an efficiency between 70 and 80% and the total concentration factor ranged between 150000 and 250000 times, depending on the requirements and the methods of choice.
Eliasson
Linda
L
Department of Cell and Organism Biology, Lund University, Sölvegatan 35, SE-223 62, Lund, Sweden.
Sydoff
Stefan
S
Everitt
Einar
E
D000327:Adsorption; D000818:Animals; D002478:Cells, Cultured; D002852:Chromatography, Ion Exchange / Q000295:instrumentation* / Q000379:methods*; D005347:Fibroblasts; D005374:Filtration / Q000295:instrumentation; D018061:Infectious pancreatic necrosis virus / Q000145:classification / Q000302:isolation & purification* / Q000472:pathogenicity; D007475:Ion Exchange Resins; D012474:Salmon; D014773:Virology / Q000379:methods; D014871:Water Microbiology
D007475:Ion Exchange Resins