Metabolism of SFZ-47 in chicken embryo by liquid chromatography-electrospray ion trap mass spectrometry.
OBJECTIVE
To develop an alternative method for investigation of drug metabolism by fertilized chicken eggs using 3H-1,2-dihydro-2-(4-methyl-phenylamino) methyl-1-pyrrolizinone (SFZ-47) as a probe drug.


METHODS
SFZ-47 (15 mg) was injected into the albumen of eggs from standardized breed chickens previously incubated for 10 d. After 72 h of further incubation, the allantoic liquid was subjected to solid phase extraction on XAD-2 columns and analyzed by liquid chromatography-electrospray ion trap mass spectrometry method.


RESULTS
Three major metabolites were identified, namely 4-(3H-1,2-dihydro-1-pyrrolizinone-2-methyl-amino) benzyl alcohol (SFZ-47-OH), 4-(3H-1,2-dihydro-1-pyrrolizinone-2-methyl-amino)-benzoic acid (SFZ-47-COOH), and its glucuronide conjugates. The metabolic profile was little different from that previously found in rabbits and dogs.


CONCLUSIONS
The result demonstrates the usefulness of the fertilized chicken egg as a convenient source of both phase I and phase II metabolites for further metabolism studies of SFZ-47.
Dong
Qing-Guang
QG
Research Center for Drug Metabolism, Jilin University, Changchun 130023, China.
Gu
Jing-Kai
JK
Zhong
Da-Fang
DF
Fawcett
J Paul
JP
Zhou
Hui
H
D000818:Animals; D002642:Chick Embryo; D002853:Chromatography, Liquid; D011758:Pyrroles / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization
C460139:3H-1,2-dihydro-2-(4-methylphenylamino)methyl-1-pyrrolizinone; D011758:Pyrroles