title: Impedance spectroscopy for monitoring ischemic injury in the intestinal mucosa.
abstract: This work evaluates the feasibility of monitoring ischemic injury in the gastrointestinal mucosa by impedance spectroscopy, using a minimally invasive intestinal catheter. The disruption of the intestinal mucosa plays a key role in the evolution of shock and is the 'motor of multiple organ failure'. Different technologies have been developed to monitor mucosal perfusion, oxygenation and/or ischemia, but no practical method exists to assess tissue damage, which may be crucial for preventing multiple organ failure. The experimental protocol of this study relied on an isobaric model of hypovolemic shock in 16 anaesthetized rabbits assigned to three groups: sham (n = 6), ischemia (n = 5) and ischemia + reperfusion (n = 5). Complex impedance spectra were recorded in the range of 0.05 to 300 kHz, with simultaneous measurements of tonometric pHi in the ileum every 30 min for 4 h. Impedance spectra were reproducible, and those of tissue under prolonged ischemia were clearly differentiable from those of normally perfused tissue. The dynamic changes in impedance did not correlate directly with either tissue perfusion or pHi, but instead correlated well with the duration of ischemia. It is concluded that impedance spectroscopy does indeed measure changes in tissue injury, and could be a very useful tool to guide therapy of patients in shock.
authors.affiliation: Escuela Militar de Graduados de Sanidad, Universidad del Ejército y Fuerza Aérca, México DF 11320, Mexico.
authors.forename: César A
authors.initials: CA
authors.lastname: González
authors.forename: Cleva
authors.initials: C
authors.lastname: Villanueva
authors.forename: Salah
authors.initials: S
authors.lastname: Othman
authors.forename: Raúl
authors.initials: R
authors.lastname: Narváez
authors.forename: Emilio
authors.initials: E
authors.lastname: Sacristán
mesh_terms: D000818:Animals; D001794:Blood Pressure; D017097:Electric Impedance; D007413:Intestinal Mucosa / Q000503:physiopathology*; D007511:Ischemia / Q000175:diagnosis* / Q000503:physiopathology*; D008297:Male; D009102:Multiple Organ Failure / Q000175:diagnosis / Q000503:physiopathology; D011817:Rabbits; D012769:Shock / Q000175:diagnosis / Q000503:physiopathology; D013057:Spectrum Analysis / Q000379:methods*