Passing of fluorescein derivatives into the hyphae of Phanerochaete chrysosporium.
Fluorescein derivatives added into the growth medium were decolorized during submerged cultivation of Phanerochaete chrysosporium. The highest decrease of absorbance A450 was observed in the growth phase regardless of the presence of inducers Tween 80 or poly(ethylene glycol) (PEG). Fluorescein linked to PEG was prepared and, after addition to cultures, shown to stimulate the production of lignin peroxidase. Passing of fluorescing substances into hyphae (observed by confocal microscopy) showed that they were concentrated on some structures inside hyphae.
Grgic
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Faculty of Chemistry and Chemical Technology, University of Ljubljana, 1000 Ljubljana, Slovenia. irena.grgic@uni-lj.si
Perdih
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A
D003470:Culture Media; D004790:Enzyme Induction; D019793:Fluorescein / Q000737:chemistry / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D025301:Hyphae / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism; D020075:Phanerochaete / Q000254:growth & development / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology; D011136:Polysorbates / Q000494:pharmacology
D003470:Culture Media; D005456:Fluorescent Dyes; D011136:Polysorbates; D011092:Polyethylene Glycols; D010544:Peroxidases; C042858:lignin peroxidase; D019793:Fluorescein