Characterization of formaldehyde-inactivated poliovirus preparations made from live-attenuated strains.
Formaldehyde-inactivated virus samples from type 1 poliovirus live-attenuated strains were prepared in the laboratory. The effect of treatment with formaldehyde on virus infectivity and immunogenicity in mice was investigated and the results compared with those from Mahoney wild-type poliovirus strain, the common type 1 component in commercial inactivated polio vaccines (IPV). Differences in the potency and specificity between these experimental vaccines were identified in both normal mice and transgenic mice expressing the human poliovirus receptor. The possible advantages/disadvantages of using live-attenuated strains for IPV production are discussed in the context of the global polio eradication initiative. A novel transgenic mouse model to study in vivo the immune protection induced by IPV preparations is described.
Martín
Javier
J
Division of Virology, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire EN6 3QG, UK.
Crossland
Graham
G
Division of Virology, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire EN6 3QG, UK.
Wood
David J
DJ
Division of Virology, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire EN6 3QG, UK.
Minor
Philip D
PD
Division of Virology, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire EN6 3QG, UK.
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002460:Cell Line; D004195:Disease Models, Animal; D005557:Formaldehyde / Q000494:pharmacology*; D006801:Humans; D007114:Immunization; D008565:Membrane Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D011051:Poliomyelitis / Q000401:mortality / Q000517:prevention & control* / Q000821:virology; D017955:Poliovirus / Q000187:drug effects / Q000276:immunology / Q000472:pathogenicity*; D011054:Poliovirus Vaccine, Inactivated / Q000008:administration & dosage* / Q000276:immunology*; D011055:Poliovirus Vaccine, Oral; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism; D014774:Virulence
D000914:Antibodies, Viral; D008565:Membrane Proteins; D011054:Poliovirus Vaccine, Inactivated; D011055:Poliovirus Vaccine, Oral; D011991:Receptors, Virus; C066100:poliovirus receptor; D005557:Formaldehyde