Dual effects of endogenous DNases on transcriptionally active and inactive chromatin.
Experiments on resting hepatocytes with inactive c-fos gene and active albumin gene. We revealed that DNA of the transcribed gene is less susceptible to the influence of endogenous Ca2+/Mg(2+)-dependent DNases in matrix-associated and highly soluble chromatin fractions. In the fraction of low soluble chromatin active gene was more accessible for DNases. Our results indicate that activity of endogenous DNases can change in the transcribed gene locus.
Borisova
N P
NP
Laboratory of Molecular Biology, Institute for Problem of Chemical Physics, Russian Academy of Sciences, Chernogolovka, Moscow region.
Kostyuk
G V
GV
Shevchenko
N A
NA
Boikov
P Ya
PY
Rudakova
E V
EV
Papina
R I
RI
Terent'ev
A A
AA
D000418:Albumins / Q000235:genetics; D000818:Animals; D002843:Chromatin / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000378:metabolism*; D005786:Gene Expression Regulation; D016762:Genes, fos; D022781:Hepatocytes / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014158:Transcription, Genetic
D000418:Albumins; D002843:Chromatin; D003851:Deoxyribonucleases