Measles virus V protein blocks interferon (IFN)-alpha/beta but not IFN-gamma signaling by inhibiting STAT1 and STAT2 phosphorylation.
Measles virus (MV), a member of the family Paramyxoviridae, encodes C and V non-structural proteins. To clarify the functions of MV C and V proteins, HeLa cell lines constitutively expressing C or V protein were established. We found that expression of V protein inhibited interferon (IFN)-alpha/beta signaling but not IFN-gamma signaling. C protein had no inhibitory effect on IFN signaling in our experimental condition. Degradation of selective signal transducers and activators of transcription (STAT) proteins was not observed in HeLa cells expressing V protein. In contrast, tyrosine phosphorylation of both STAT1 and STAT2 was inhibited in these cells after IFN-beta stimulation.
Takeuchi
Kaoru
K
Department of Infection Biology, Institute of Basic Medical Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan. ktakeuch@md.tsukuba.ac.jp
Kadota
Shin-ich
SI
Takeda
Makoto
M
Miyajima
Naoko
N
Nagata
Kyosuke
K
D002460:Cell Line; D006367:HeLa Cells; D006801:Humans; D016898:Interferon-alpha / Q000037:antagonists & inhibitors* / Q000378:metabolism; D016899:Interferon-beta / Q000037:antagonists & inhibitors* / Q000378:metabolism; D007371:Interferon-gamma / Q000378:metabolism*; D010750:Phosphoproteins / Q000494:pharmacology*; D010766:Phosphorylation; D015398:Signal Transduction / Q000187:drug effects*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation / Q000187:drug effects*; D014764:Viral Proteins / Q000494:pharmacology*
D016898:Interferon-alpha; D010750:Phosphoproteins; D015534:Trans-Activators; C069181:V protein, measles virus; D014764:Viral Proteins; D016899:Interferon-beta; D007371:Interferon-gamma