Tetraspanin CD151 regulates alpha6beta1 integrin adhesion strengthening.
The tetraspanin CD151 molecule associates specifically with laminin-binding integrins, including alpha6beta1. To probe strength of alpha6beta1-dependent adhesion to laminin-1, defined forces (0-1.5 nN) were applied to magnetic laminin-coated microbeads bound to NIH 3T3 cells. For NIH 3T3 cells bearing wild-type CD151, adhesion strengthening was observed, as bead detachment became more difficult over time. In contrast, mutant CD151 (with the C-terminal region replaced) showed impaired adhesion strengthening. Static cell adhesion to laminin-1, and detachment of beads coated with fibronectin or anti-alpha6 antibody were all unaffected by CD151 mutation. Hence, CD151 plays a key role in selectively strengthening alpha6beta1 integrin-mediated adhesion to laminin-1.
Lammerding
Jan
J
Biological Engineering Division, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Kazarov
Alexander R
AR
Huang
Hayden
H
Lee
Richard T
RT
Hemler
Martin E
ME
D016475:3T3 Cells; D000818:Animals; D015703:Antigens, CD / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D002138:Calibration; D002448:Cell Adhesion; D002460:Cell Line; D039121:Integrin alpha6beta1 / Q000378:metabolism*; D007797:Laminin / Q000378:metabolism*; D008280:Magnetics; D051379:Mice; D009154:Mutation; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D013314:Stress, Mechanical; D013696:Temperature; D060185:Tetraspanin 24; D013997:Time Factors
D015703:Antigens, CD; C554716:Cd151 protein, mouse; D039121:Integrin alpha6beta1; D007797:Laminin; D060185:Tetraspanin 24; C121346:laminin 1