title: Function and glycosylation of plant-derived antiviral monoclonal antibody.
abstract: Plant genetic engineering led to the production of plant-derived mAb (mAbP), which provides a safe and economically feasible alternative to the current methods of antibody production in animal systems. In this study, the heavy and light chains of human anti-rabies mAb were expressed and assembled in planta under the control of two strong constitutive promoters. An alfalfa mosaic virus untranslated leader sequence and Lys-Asp-Glu-Leu (KDEL) endoplasmic reticulum retention signal were linked at the N and C terminus of the heavy chain, respectively. mAbP was as effective at neutralizing the activity of the rabies virus as the mammalian-derived antibody (mAbM) or human rabies Ig (HRIG). The mAbP contained mainly oligomannose type N-glycans (90%) and had no potentially antigenic alpha(1,3)-linked fucose residues. mAbP had a shorter half-life than mAbM. The mAbP was as efficient as HRIG for post-exposure prophylaxis against rabies virus in hamsters, indicating that differences in N-glycosylation do not affect the efficacy of the antibody in this model.
authors.affiliation: Biotechnology Foundation Laboratories at Thomas Jefferson University, 1020 Locust Street, JAH, Philadelphia, PA 19107, USA.
authors.forename: Kisung
authors.initials: K
authors.lastname: Ko
authors.forename: Yoram
authors.initials: Y
authors.lastname: Tekoah
authors.forename: Pauline M
authors.initials: PM
authors.lastname: Rudd
authors.forename: David J
authors.initials: DJ
authors.lastname: Harvey
authors.forename: Raymond A
authors.initials: RA
authors.lastname: Dwek
authors.forename: Sergei
authors.initials: S
authors.lastname: Spitsin
authors.forename: Cathleen A
authors.initials: CA
authors.lastname: Hanlon
authors.forename: Charles
authors.initials: C
authors.lastname: Rupprecht
authors.forename: Bernhard
authors.initials: B
authors.lastname: Dietzschold
authors.forename: Maxim
authors.initials: M
authors.lastname: Golovkin
authors.forename: Hilary
authors.initials: H
authors.lastname: Koprowski
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D006224:Cricetinae; D018076:DNA, Complementary / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004721:Endoplasmic Reticulum / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D017343:Genes, Plant; D006031:Glycosylation; D008647:Mesocricetus; D051379:Mice; D008807:Mice, Inbred BALB C; D008957:Models, Genetic; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified; D011134:Polysaccharides / Q000737:chemistry; D011401:Promoter Regions, Genetic; D017434:Protein Structure, Tertiary; D011820:Rabies virus / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors; D014026:Nicotiana / Q000235:genetics
chemical_list: D000911:Antibodies, Monoclonal; D018076:DNA, Complementary; D011134:Polysaccharides