title: Cardiotoxicity of trastuzumab (herceptin) in chick embryos.
abstract: The cardiac toxicity of trastuzumab was studied in chick embryos. Fertilized eggs of White Leghorns were incubated and investigated. Trastuzumab 5 mg/egg (low dose) or 15 mg/egg (high dose) was injected into the air sac of a fertilized egg on the 16th day of incubation. Electrocardiograms (ECGs) were recorded 0 to 60 min after the injection. After low dosing of trastuzumab, the heart rate was not different compared with the control. However, the heart rate was significantly decreased by high dosing of trastuzumab. In addition, arrhythmia was produced by high dosing of trastuzumab. These findings indicate that trastuzumab has a marked dose- and time-dependent influence on the heart rate in chick embryos.
authors.affiliation: Division of Clinical Pharmacy, Kyoritsu College of Pharmacy, 1-5-30 Shibakoen, Minato-ku, Tokyo 105-8512, Japan. yoshiyama-yj@kyoritsu-ph.ac.jp
authors.forename: Yuji
authors.initials: Y
authors.lastname: Yoshiyama
authors.forename: Takashi
authors.initials: T
authors.lastname: Sugiyama
authors.forename: Motoko
authors.initials: M
authors.lastname: Kanke
mesh_terms: D023401:Animal Use Alternatives / Q000379:methods*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000633:toxicity*; D061067:Antibodies, Monoclonal, Humanized; D000970:Antineoplastic Agents / Q000633:toxicity*; D002642:Chick Embryo; D004562:Electrocardiography; D006321:Heart / Q000187:drug effects* / Q000196:embryology; D006339:Heart Rate / Q000187:drug effects; D066298:In Vitro Techniques; D000068878:Trastuzumab
chemical_list: D000911:Antibodies, Monoclonal; D061067:Antibodies, Monoclonal, Humanized; D000970:Antineoplastic Agents; D000068878:Trastuzumab