[Effect of gamma-aminobutyric shunt metabolites on NAD-, NADP-isocitrate dehydrogenases, and aconitate hydratase activities in higher plants].
We studied the influence of metabolites of gamma-aminobutyric shunt of the tricarboxylic acid cycle on the activities of aconitate hydratase (EC.4.2.1.3) as well as NAD- and NADP-specific isocitrate dehydrogenases (EC.1.1.1.41 and EC.1.1.1.42, respectively) using purified enzyme preparations from pea leaves (Pisum sativum L.) and maize scutellum (Zea mays L.). gamma-Aminobutyric acid and succinate proved to have no significant effect on these enzymes, while 0.1-0.2 mM glutamate considerably activated NADP isocitrate dehydrogenase from the both sources, particularly, at unsaturating concentration of the substrate. Succinic semialdehyde stimulated the activities of aconitate hydratase and NADP isocitrate dehydrogenase. The obtained data points to a similar pattern of the effect of intermediates of gamma-aminobutyric shunt on the studied enzymatic activities for both photosynthetic tissues (pea leaves) and those with acidifying, transport, and digestive functions (maize scutellum). However, the absence of pronounced control effects of most metabolites on the studied enzymes allows us to assign them to a relatively inert pool of metabolites.
Pinheiro de Carvalho
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MA
Research Center of Biological and Geological Sciences, University of Madeira, 9000-081 Funchal, Portugal. quercus@uma.pt
Popova
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TN
Dush Santos
T M M
TM
Zherebtsov
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NA
D000154:Aconitate Hydratase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000378:metabolism* / Q000494:pharmacology; D007521:Isocitrate Dehydrogenase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D019684:Magnoliopsida / Q000201:enzymology* / Q000502:physiology; D018532:Pisum sativum / Q000201:enzymology / Q000502:physiology; D018515:Plant Leaves / Q000201:enzymology / Q000502:physiology; D003313:Zea mays / Q000201:enzymology / Q000502:physiology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism* / Q000494:pharmacology
D004791:Enzyme Inhibitors; D005680:gamma-Aminobutyric Acid; D007521:Isocitrate Dehydrogenase; C039355:isocitrate dehydrogenase (NADP+); D000154:Aconitate Hydratase