title: Regulation by nectin of the velocity of the formation of adherens junctions and tight junctions.
abstract: Cadherins are key Ca(2+)-dependent cell-cell adhesion molecules at adherens junctions (AJs) in fibroblasts and epithelial cells, whereas claudins are key Ca(2+)-independent cell-cell adhesion molecules at tight junctions (TJs) in epithelial cells. The formation and maintenance of TJs are dependent on the formation and maintenance of AJs. Nectins are Ca(2+)-independent immunoglobulin-like cell-cell adhesion molecules which comprise a family of four members, nectin-1, -2, -3, and -4, and are involved in the formation of AJs in cooperation with cadherins, and the subsequent formation of TJs. We show here that the velocity of the formation of the E-cadherin-based AJs is increased by overexpression of nectin-1 and is reduced by addition of the nectin-1 inhibitors to the medium in L cells stably expressing E-cadherin and Madin-Darby canine kidney cells. Moreover, the velocity of the formation of the claudin-based TJs is increased by overexpression of nectin-1 and is reduced by addition of the nectin-1 inhibitors to the medium in Madin-Darby canine kidney cells. These results indicate that nectins regulate the velocity of the formation of the E-cadherin-based AJs and the subsequent formation of the claudin-based TJs.
authors.affiliation: Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine/Faculty of Medicine, Osaka University, 565-0871, Suita, Japan.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Honda
authors.forename: Kazuya
authors.initials: K
authors.lastname: Shimizu
authors.forename: Atsunori
authors.initials: A
authors.lastname: Fukuhara
authors.forename: Kenji
authors.initials: K
authors.lastname: Irie
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Takai
mesh_terms: D022005:Adherens Junctions / Q000502:physiology*; D000818:Animals; D015820:Cadherins / Q000502:physiology; D002448:Cell Adhesion / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000037:antagonists & inhibitors / Q000502:physiology*; D002460:Cell Line; D062445:Claudin-1; D004285:Dogs; D016547:Kinesins; D007700:Kinetics; D007739:L Cells; D008565:Membrane Proteins / Q000502:physiology; D051379:Mice; D008840:Microfilament Proteins / Q000502:physiology; D009218:Myosins; D000075983:Nectins; D019108:Tight Junctions / Q000502:physiology*
chemical_list: C490657:Afdn protein, mouse; D015820:Cadherins; D015815:Cell Adhesion Molecules; D062445:Claudin-1; C568113:Cldn1 protein, mouse; D008565:Membrane Proteins; D008840:Microfilament Proteins; C000619015:Nectin1 protein, mouse; D000075983:Nectins; C108835:afadin; D009218:Myosins; D016547:Kinesins