Short-term exposure to 17 alpha-ethynylestradiol decreases the fertility of sexually maturing male rainbow trout (Oncorhynchus mykiss).
The synthetic estrogen 17 alpha-ethynylestradiol (EE2) is a commonly used oral contraceptive that has been increasingly detected in sewage effluents. This study determined whether EE2 exposure adversely affected reproduction in sexually maturing male rainbow trout (Oncorhynchus mykiss). We exposed male trout to graded water concentrations of EE2 (10, 100, and 1,000 ng/ L) for 62 d leading up to the time of spawning. Semen and blood plasma samples were removed from each fish. Semen was used to fertilize groups of eggs from one nonexposed female. As a measure of fertility, eggs were incubated for 28 d after fertilization to determine the proportion that attained the eyed stage of embryonic development. Additional endpoints also measured included sperm motility, spermatocrit, gonadosomatic and hepatosomatic indices, testis histology, and circulating plasma levels of the sex steroids 17 alpha, 20 beta-dihydroxyprogesterone (17,20-DHP) and 11-ketotestosterone (11-KT). Exposure to 1,000 ng/L of EE2 caused complete mortality of the treatment group by day 57. Exposure to lower EE2 water concentrations (10 and 100 ng/L) caused an increase in sperm density, while a significant reduction in testis mass was observed only in the 100-ng/L exposure group. Most significantly, semen harvested from fish exposed to 10 and 100 ng/L EE2 caused an approximately 50% reduction in the number of eggs attaining the eyed stage of embryonic development. Plasma levels of 17,20-DHP in exposed fish were roughly twice the level of the controls, while levels of 11-KT were significantly reduced in fish exposed to 100 ng/L EE2. These results suggest that sexually maturing male rainbow trout are susceptible to detrimental reproductive effects of short-term exposures to environmentally relevant levels of EE2.
Schultz
Irvin R
IR
Battelle MSL-PNNL, 1529 West Sequim Bay Road, Sequim, Washington 98382, USA. ir_schultz@pnl.gov
Skillman
Ann
A
Nicolas
Jean-Marc
JM
Cyr
Daniel G
DG
Nagler
James J
JJ
D000818:Animals; D004781:Environmental Exposure; D004997:Ethinyl Estradiol / Q000031:analogs & derivatives / Q000506:poisoning*; D005260:Female; D005306:Fertilization / Q000187:drug effects; D005393:Fish Diseases / Q000139:chemically induced*; D006908:Hydroxyprogesterones / Q000097:blood; D007246:Infertility / Q000139:chemically induced*; D008297:Male; D012661:Semen / Q000187:drug effects; D012741:Sexual Maturation / Q000187:drug effects*; D013091:Spermatogenesis / Q000187:drug effects; D013737:Testis / Q000187:drug effects / Q000648:ultrastructure; D013739:Testosterone / Q000031:analogs & derivatives* / Q000097:blood; D013997:Time Factors; D014337:Trout / Q000502:physiology*; D014874:Water Pollutants, Chemical / Q000506:poisoning*
D006908:Hydroxyprogesterones; D014874:Water Pollutants, Chemical; C021012:17 alpha,20 beta-dihydroxypregn-4-en-3-one; D013739:Testosterone; D004997:Ethinyl Estradiol; C003600:11-ketotestosterone