Multidimensional gas chromatography-mass spectrometry for tracer studies of fatty acid metabolism via stable isotopes in cultured human trophoblast cells.
The determination of placental fatty acid metabolism using stable isotope-labeled tracers was investigated in the human placental choriocarcinoma (JAR) cell line. Stable isotope incorporation was measured by MDGC-MS. The cultured trophoblast cells incorporated and metabolized the essential fatty acids to long-chain polyunsaturated fatty acids. The described method enables the detection of a low Delta(6)-desaturase activity in this human placental cell line. The developed MDGC-MS method allows the assessment of long-chain polyunsaturated fatty acid biosynthesis in cultured cells with high sensitivity and selectivity. In this respect, tracer studies with MDGC-MS will be a powerful tool to clarify the significance of placental fatty acid metabolism.
Muth
Alexandra
A
Department of Food Chemistry, J.W. Goethe-University, Biocenter, Marie-Curie-Strasse 9, D-60439 Frankfurt/Main, Germany.
Mosandl
Armin
A
Bursen
Adelheid
A
Marschalek
Rolf
R
Sewell
Adrian C
AC
Böhles
Hans
H
D002478:Cells, Cultured; D044943:Fatty Acid Desaturases / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D007554:Isotopes; D050602:Linoleoyl-CoA Desaturase; D010920:Placenta / Q000201:enzymology; D012680:Sensitivity and Specificity
D005227:Fatty Acids; D007554:Isotopes; D044943:Fatty Acid Desaturases; D050602:Linoleoyl-CoA Desaturase