Effects of resveratrol and 4-hexylresorcinol on hydrogen peroxide-induced oxidative DNA damage in human lymphocytes.
The protective effects of resveratrol and 4-hexylresorcinol against oxidative DNA damage in human lymphocytes induced by hydrogen peroxide were investigated. Resveratrol and 4-hexylresorcinol showed no cytotoxicity to human lymphocytes at the tested concentration (10-100 microM). In addition, DNA damage in human lymphocytes induced by H2O2 was inhibited by resveratrol and 4-hexylresorcinol. Resveratrol and 4-hexylresorcinol at concentrations of 10-100 microM induced an increase in glutathione (GSH) levels in a concentration-dependent manner. Moreover, these two compounds also induced activity of glutathione peroxidase (GPX) and glutathione reductase (GR). The activity of glutathione-S-transferase (GST) in human lymphocytes was induced by resveratrol. Resveratrol and 4-hexylresorcinol inhibited the activity of catalase (CAT). These data indicate that the inhibition of resveratrol and 4-hexylresorcinol on oxidative DNA damage in human lymphocytes induced by H2O2 might be attributed to increase levels of GSH and modulation of antioxidant enzymes (GPX, GR and GST).
Yen
Gow-Chin
GC
Department of Food Science, National Chung Hsing University, 250 Kuokuang Road, Taichung 40227, Taiwan. gcyen@mail.nchu.edu.tw
Duh
Pin-Der
PD
Lin
Chun-Wei
CW
D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000187:drug effects / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000187:drug effects / Q000378:metabolism; D005980:Glutathione Reductase / Q000187:drug effects / Q000378:metabolism; D005982:Glutathione Transferase / Q000187:drug effects / Q000378:metabolism; D006604:Hexylresorcinol / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000008:administration & dosage*; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D016877:Oxidants / Q000008:administration & dosage; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000187:drug effects / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D013997:Time Factors
D000975:Antioxidants; D016877:Oxidants; D013267:Stilbenes; D006861:Hydrogen Peroxide; D010544:Peroxidases; D002374:Catalase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D005978:Glutathione; D000077185:Resveratrol; D006604:Hexylresorcinol