Bacteriocin diversity in Bacillus sphaericus.
Nondenaturing polyacrylamide gel electrophoresis revealed the presence of diversity among bacteriocins produced by strains of Bacillus sphaericus. Bacteriocin bands of six strains (pathogenic and non pathogenic) were found to be located just below the stacking gel. However, in two other strains (1 pathogenic and 1 collection strain) more than one protein band with bacteriocin activity were seen in the middle of resolving gel. In bacteriocin-treated cultures, electron-microscopy studies revealed the growth of lysedswollen ghost cells, and loss of viability among sensitive strains.
Cetinkaya
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S
Department of Biology, Faculty of Science, Ankara University, 061 00 Ankara, Turkey.
Osmanağaoğlu
O
O
Cökmüş
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C
D000818:Animals; D001407:Bacillus / Q000187:drug effects / Q000378:metabolism* / Q000472:pathogenicity; D001430:Bacteriocins / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D009033:Culicidae / Q000382:microbiology; D004591:Electrophoresis, Polyacrylamide Gel; D007814:Larva / Q000382:microbiology; D008854:Microscopy, Electron
D001430:Bacteriocins