Evaluation of Tanacetum larvatum for an anti-inflammatory activity and for the protection against indomethacin-induced ulcerogenesis in rats.
Oral administration of the chloroform extract from Tanacetum larvatum (Griseb. ex Pant.) Kanitz caused a dose-dependent anti-inflammatory effect in the carrageenan-induced rat paw oedema test. The obtained anti-inflammatory effect was 8.6, 32.8, 37.0 and 49.5% for the extract doses of 25, 50, 100 and 200mg/kg, respectively, being statistically significant at a dose of 50mg/kg. Indomethacin had a strong anti-inflammatory effect of 73.4% at a dose of 8mg/kg, but large gastric lesions were detected. When the plant extract in the highest tested dose (200mg/kg) was concomitantly given with indomethacin, the anti-inflammatory effect was slightly enhanced, but the gastric lesions were significantly reduced. The anti-inflammatory and anti-ulcer activity may be mainly due to the inhibition of DNA binding of the transcription factor NF-kappaB by components of the plant extract. This was proven in an electrophoretic mobility shift assay at a concentration of 50microg/ml. Due to its anti-inflammatory as well as anti-ulcer effects, Tanacetum larvatum should especially be used combined with those drugs that are known both for their strong anti-inflammatory activities and the ulcerogenic side effects such as NSAIDs.
Petrovic
Silvana D
SD
Department of Pharmacognosy, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, Yugoslavia. silvana@afrodita.rcub.bg.ac.yu
Dobric
Silva
S
Bokonjic
Dubravko
D
Niketic
Marjan
M
García-Piñeres
Alfonso
A
Merfort
Irmgard
I
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002351:Carrageenan; D004247:DNA / Q000378:metabolism; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D006614:Hindlimb; D006801:Humans; D007213:Indomethacin; D019169:Jurkat Cells; D008297:Male; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D010437:Peptic Ulcer / Q000139:chemically induced / Q000188:drug therapy / Q000517:prevention & control*; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D031203:Tanacetum / Q000737:chemistry*
D000893:Anti-Inflammatory Agents; D016328:NF-kappa B; D010936:Plant Extracts; D002351:Carrageenan; D004247:DNA; D007213:Indomethacin