[Cervical carcinoma progression-associated genetic alterations on chromosome 6].
To identify the loci associated with progression of cervical carcinoma, chromosome 6 regions were tested for loss of heterozygosity. Detailed analysis with 28 microsatellite markers revealed a high frequency of allelic deletions for several loci of the short (6p25, 6p22, 6p21.3) and long (6q14, 6q16-21, 6q23-24, 6q25, 6q27) arms of chromosome 6. Examination of 37 microdissected carcinoma and 22 cervical dysplasia specimens revealed allelic deletions from the HLA class I-III genes (6p22-21.3) and subtelomeric locus 6p25 were found in more than 40% dysplasia specimens. With multiple microdissection of cryosections, genetic heterogeneity of squamous cervical carcinoma was analyzed, and clonal and subclonal allelic deletions from chromosome 6 were identified. Half of the tumors had clonal allelic deletion of D6S273 (6p21.3), which is in a Ly6G6D (MEGT1) intron in the HLA class III gene locus. The frequency of allelic deletions from the chromosome 6 long arm was no more than 20% in dysplasias. Allelic deletions from two loci, 6q14 and 6q16-21, were for the first time associated with invasion and metastasis in cervical carcinoma.
Mazurenko
N N
NN
Institute of Carcinogenesis, Blokhin Cancer Research Center, Russian Academy of Medical Sciences. nnmazurenko@mail.ru
Beliakov
I S
IS
Bliev
A Iu
AIu
Guo
Z
Z
Hu
X
X
Vinokurova
S V
SV
Bidzhieva
B A
BA
Pavlova
L S
LS
Ponten
J
J
Kiselev
F L
FL
D002896:Chromosomes, Human, Pair 6; D018450:Disease Progression; D005260:Female; D006680:HLA Antigens / Q000235:genetics; D006801:Humans; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats; D009361:Neoplasm Invasiveness; D017384:Sequence Deletion; D002578:Uterine Cervical Dysplasia / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology
D006680:HLA Antigens