title: Expression in cereal plants of genes that inactivate Fusarium mycotoxins.
abstract: Trichothecene 3-O-acetyltransferase (encoded by Tri101) inactivates the virulence factor of the cereal pathogen Fusarium graminearum. Zearalenone hydrolase (encoded by zhd101) detoxifies the oestrogenic mycotoxin produced by the same pathogen. These genes were introduced into a model monocotyledon rice plant to evaluate their usefulness for decontamination of mycotoxins. The strong and constitutive rice Act1 promoter did not cause accumulation of TRI101 protein in transgenic rice plants. In contrast, the same promoter was suitable for transgenic production of ZHD101 protein; so far, five promising T0 plants have been generated. Low transgenic expression of Tri101 was suggested to be increased by addition of an omega enhancer sequence upstream of the start codon.
authors.affiliation: Laboratory for Remediation Research, Plant Science Center, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Arisa
authors.initials: A
authors.lastname: Higa
authors.forename: Makoto
authors.initials: M
authors.lastname: Kimura
authors.forename: Kouhei
authors.initials: K
authors.lastname: Mimori
authors.forename: Tetsuko
authors.initials: T
authors.lastname: Ochiai-Fukuda
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tokai
authors.forename: Naoko
authors.initials: N
authors.lastname: Takahashi-Ando
authors.forename: Takumi
authors.initials: T
authors.lastname: Nishiuchi
authors.forename: Tomoko
authors.initials: T
authors.lastname: Igawa
authors.forename: Makoto
authors.initials: M
authors.lastname: Fujimura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hamamoto
authors.forename: Ron
authors.initials: R
authors.lastname: Usami
authors.forename: Isamu
authors.initials: I
authors.lastname: Yamaguchi
mesh_terms: D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism; D002523:Edible Grain / Q000235:genetics* / Q000382:microbiology; D004742:Enhancer Elements, Genetic; D005656:Fungal Proteins; D005670:Fusarium / Q000737:chemistry / Q000821:virology; D006867:Hydrolases / Q000235:genetics / Q000378:metabolism; D009183:Mycotoxins / Q000037:antagonists & inhibitors*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D014170:Transformation, Genetic
chemical_list: D005656:Fungal Proteins; D009183:Mycotoxins; D000123:Acetyltransferases; C110205:trichothecene 3-O-acetyltransferase; D006867:Hydrolases; C460562:Zhd101 protein, Bionectria ochroleuca