Comparison of fluidized bed and ultrasonic cell-retention systems for high cell density mammalian cell culture.
Economically viable biopharmaceutical production is to a high degree dependent on high product yields and stable fermentation systems that are easy to handle. In the current study we have compared two different fermentation systems for the production of recombinant protein from CHO cells. Both systems are fully scaleable and can be used for industrial high cell density bioprocesses. As a model cell line we have used a recombinant CHO cell line producing the enzyme arylsulfatase B (ASB). CHO cells were cultivated as adherent cell culture attached on Cytoline macroporous microcarrier (Amersham Biosciences, Sweden) using a Cytopilot Mini fluidized bed bioreactor (FBR, Vogelbusch-Amersham Biosciences, Austria) and as suspension culture using a stirred tank bioreactor equipped with a BioSep ultrasonic resonator based cell separation device (Applikon, The Netherlands). Both systems are equally well-suited for stable, long-term high cell density perfusion cell culture and provide industrial scalability and high yields. For products such as the recombinant ASB, high perfusion rates and therefore short product bioreactor residence times may be of additional benefit.
Dürrschmid
Markus P
MP
Institute of Applied Microbiology (IAM), University of Natural Resources and Applied Life Sciences, Muthgasse 18, A-1190 Vienna, Austria. office@iam.boku.ac.at
Landauer
Karlheinz
K
Simic
Gordana
G
Klug
Helga
H
Keijzer
Timo
T
Trampler
Felix
F
Oudshoorn
Arthur
A
Gröschl
Martin
M
Müller
Dethardt
D
Doblhoff-Dier
Otto
O
D000818:Animals; D019149:Bioreactors; D016466:CHO Cells; D002448:Cell Adhesion / Q000502:physiology / Q000528:radiation effects; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods*; D002455:Cell Division / Q000502:physiology* / Q000528:radiation effects; D006224:Cricetinae; D003412:Cricetulus; D019544:Equipment Failure Analysis; D020051:N-Acetylgalactosamine-4-Sulfatase / Q000096:biosynthesis*; D010865:Pilot Projects; D011994:Recombinant Proteins / Q000096:biosynthesis; D012212:Rheology / Q000295:instrumentation / Q000379:methods*; D014465:Ultrasonics
D011994:Recombinant Proteins; D020051:N-Acetylgalactosamine-4-Sulfatase