title: Trypan blue-assisted peeling of the internal limiting membrane during macular hole surgery.
abstract: OBJECTIVE
  To evaluate the outcome of surgeries for macular holes using trypan blue to facilitate delamination of the internal limiting membrane.
  
  
  METHODS
  A retrospective series of 18 patients.
  
  
  METHODS
  All patients underwent a three-port pars plana vitrectomy with internal limiting membrane delamination using trypan blue 0.06% to assist visualization. The main outcome measures were postoperative visual acuity and clinically evident retinal pigment epithelial changes.
  
  
  RESULTS
  The median preoperative visual acuity was 20/200, and the median postoperative visual acuity was 20/70. All but one macular hole were closed after surgery (94%). At the last recorded follow-up, 10 patients (56%) had improved visual acuity of at least two chart lines. No adverse reaction related to trypan blue was observed up to 1 year postsurgery.
  
  
  CONCLUSIONS
  Trypan blue staining of internal limiting membrane may accomplish more complete dissections of the internal limiting membrane and better surgical and visual outcomes.
authors.affiliation: Ophthalmology Department, Centre Hospitalier Université de Montréal-Pavillon Notre-Dame, Montréal, Québec, Canada. perrier.manuel@sympatico.ca
authors.forename: Manuel
authors.initials: M
authors.lastname: Perrier
authors.forename: Mikaël
authors.initials: M
authors.lastname: Sébag
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004396:Coloring Agents; D019773:Epiretinal Membrane / Q000175:diagnosis* / Q000601:surgery*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012167:Retinal Perforations / Q000601:surgery*; D012189:Retrospective Studies; D013194:Staining and Labeling / Q000379:methods; D014343:Trypan Blue; D014792:Visual Acuity; D014821:Vitrectomy
chemical_list: D004396:Coloring Agents; D014343:Trypan Blue