Simultaneous analysis of seventeen chemical ingredients of Ligusticum chuanxiong by on-line high performance liquid chromatography-diode array detector-mass spectrometry.
An on-line high performance liquid chromatography (HPLC)-diode array detector (DAD)-mass spectrometry (MS) analytical method has been developed to simultaneously separate and identify seventeen main constituents of Chuanxiong ( Ligusticum chuanxiong Hort.), a traditional Chinese medicinal herb. In three Chuanxiong samples, nine compounds were unequivocally determined as vanillin (1), ferulic acid (2), senkyunolide I (4), senkyunolide H (5), senkyunolide A (6), coniferyl ferulate (7), Z-ligustilide (8), neocnidilide (9) and 3-butylidenephthalide (10) by comparing their t R, UV, and MS data with those obtained for the authentic compounds. Furthermore, additional eight compounds were tentatively identified as senkyunolide J (11), senkyunolide F (12), 3-butylphthalide (13), cnidilide (14), riligustilide (15) or Z,Z'-6,8',7,3'-diligustilide (16), tokinolide B (17), levistolide A (18) and senkyunolide P (19), based on their MS data and the comparison of their UV spectra with the published references. This is the first report to describe the development of an on-line HPLC-DAD-MS method to simultaneously analyse seventeen main constituents present in Chuanxiong and to construct the chemical profiles of various Chuanxiong samples.
Li
Song-Lin
SL
Department of Pharmacology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, N. T., Hong Kong SAR.
Chan
Sunny Sun-Kin
SS
Lin
Ge
G
Ling
Lei
L
Yan
Ru
R
Chung
Hoi-Sing
HS
Tam
Yun-Kau
YK
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal; D006801:Humans; D030004:Ligusticum; D013058:Mass Spectrometry; D008516:Medicine, Chinese Traditional; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; C101574:chuangxiong extract