Inability of outer-surface protein C (OspC)-primed mice to elicit a protective anamnestic immune response to a tick-transmitted challenge of Borrelia burgdorferi.
A one-inoculation regimen of recombinant outer-surface protein C (OspC), which has been demonstrated to elicit protective immunity against a tick-borne challenge of Borrelia burgdorferi, was administered to outbred mice. Following seroconversion, the serum antibody titre against OspC was allowed to wane with time until there was little or no detection of anti-OspC antibodies by immunoblot. The mice were then challenged with an infectious dose of B. burgdorferi by tick transmission. Eleven of 12 OspC-primed mice subsequently became infected by B. burgdorferi, demonstrating that a protective anamnestic response was not generated in these mice following the introduction of infectious OspC-expressing spirochaetes.
Gilmore
Robert D
RD
Molecular Bacteriology Section1 and Lyme Disease Vector Section2, Bacterial Zoonoses Branch, Division of Vector-Borne Infectious Diseases (DVBID), National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, US Department of Health and Human Services, Fort Collins, CO, USA 3Department of Biology, Centocor Inc., Malvern, PA, USA.
Bacon
Rendi M
RM
Molecular Bacteriology Section1 and Lyme Disease Vector Section2, Bacterial Zoonoses Branch, Division of Vector-Borne Infectious Diseases (DVBID), National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, US Department of Health and Human Services, Fort Collins, CO, USA 3Department of Biology, Centocor Inc., Malvern, PA, USA.
Carpio
Amber M
AM
Molecular Bacteriology Section1 and Lyme Disease Vector Section2, Bacterial Zoonoses Branch, Division of Vector-Borne Infectious Diseases (DVBID), National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, US Department of Health and Human Services, Fort Collins, CO, USA 3Department of Biology, Centocor Inc., Malvern, PA, USA.
Piesman
Joseph
J
Molecular Bacteriology Section1 and Lyme Disease Vector Section2, Bacterial Zoonoses Branch, Division of Vector-Borne Infectious Diseases (DVBID), National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, US Department of Health and Human Services, Fort Collins, CO, USA 3Department of Biology, Centocor Inc., Malvern, PA, USA.
Dolan
Marc C
MC
Molecular Bacteriology Section1 and Lyme Disease Vector Section2, Bacterial Zoonoses Branch, Division of Vector-Borne Infectious Diseases (DVBID), National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, US Department of Health and Human Services, Fort Collins, CO, USA 3Department of Biology, Centocor Inc., Malvern, PA, USA.
Mbow
M Lamine
ML
Molecular Bacteriology Section1 and Lyme Disease Vector Section2, Bacterial Zoonoses Branch, Division of Vector-Borne Infectious Diseases (DVBID), National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, US Department of Health and Human Services, Fort Collins, CO, USA 3Department of Biology, Centocor Inc., Malvern, PA, USA.
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D000942:Antigens, Bacterial; D001096:Arachnid Vectors / Q000382:microbiology*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D025065:Borrelia burgdorferi / Q000166:cytology / Q000276:immunology*; D008193:Lyme Disease / Q000276:immunology* / Q000517:prevention & control / Q000635:transmission; D051379:Mice; D018448:Models, Immunological; D010641:Phenotype; D011994:Recombinant Proteins / Q000276:immunology; D013987:Ticks / Q000382:microbiology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; C079616:OspC protein; D011994:Recombinant Proteins