Effect of D-003 on isoproterenol-induced myocardial necrosis in rats.
D-003 is a mixture of high-molecular-weight aliphatic primary acids purified from sugar cane wax with antiplatelet and cholesterol-lowering effects. Cardiac lesions induced by isoproterenol (ISO) are characterized by myocardial necrosis and exudative infiltration. The objective of this study was to determine whether D-003 shows protective effects against ISO-induced myocardial necrosis in rats. Effects of orally administered single doses of D-003 (25-400 mg/kg) and acetylsalicylic acid (ASA, 30 mg/kg), as well as repeated doses of D-003 (5-200 mg/kg), on characteristic markers of ISO-induced myocardial necrosis in rats were investigated. D-003 administered as single doses dose-dependently decreased necrosis area, percent of infarct area, and the presence of polymorphonuclear cells (PMNs) in myocardial tissue, but only the reductions induced by 200 and 400 mg/kg were significant. Oral acute treatment with ASA also decreased necrosis area and percent of infarct area, but the occurrence of PMNs was unchanged. D-003 administered repeatedly for 10 days also decreased all myocardial necrosis indicators in a dose-dependent manner, with results effective from 25 mg/kg to the highest dose tested, indicating that the repeated dose scheme was more effective to prevent the damage. It is concluded that D-003 shows a protective effect on the myocardial necrosis induced by ISO in rats.
Carbajal
Daisy
D
Department of Pharmacology, Center of Natural Products, National Center for Scientific Research, Havana, Cuba. clinica@enet.cu
Noa
Miriam
M
Molina
Vivian
V
Arruzazabala
Maria Lourdes
ML
Más
Rosa
R
Mendoza
Sarahí
S
González
Jorge
J
D000818:Animals; D001241:Aspirin / Q000008:administration & dosage; D004195:Disease Models, Animal; D005227:Fatty Acids / Q000008:administration & dosage / Q000627:therapeutic use*; D007545:Isoproterenol; D008297:Male; D009203:Myocardial Infarction / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D009206:Myocardium / Q000473:pathology*; D009336:Necrosis; D009504:Neutrophils / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley
C418063:D-003 sugarcane wax acid mixture; D005227:Fatty Acids; D007545:Isoproterenol; D001241:Aspirin