High glucose enhance expression of matrix metalloproteinase-2 in smooth muscle cells.
OBJECTIVE
To investigate the effects of high glucose on expression of matrix metalloproteinase-2 (MMP-2) in rat aortic smooth muscle cells and the influence of matrix remodeling on atherogenesis in diabetic patients.


METHODS
The smooth muscle cells were cultured from the thoracic aorta of Sprague-Dawley (SD) rat. MMP-2 mRNA was determined by reverse transcriptase-polymerase chain reaction (RT-PCR), MMP-2 protein was measured by Western blotting, and MMP-2 activity in conditioned medium was observed by zymography.


RESULTS
In comparison with the control, there was no difference in the expression of MMP-2 when glucose concentration was 1 g/L, whereas MMP-2 activity in smooth muscle cells was significantly increased by the glucose 5 g/L (P<0.01).


CONCLUSIONS
High glucose enhanced the expression and activity of MMP-2 in smooth muscle cells, which may provide an explanation for the phenomenon that diabetes patients are prone to have atherosclerotic lesions.
Hao
Feng
F
Department of Cardiology, Affiliated Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China. windfengfei@etang.com
Yu
Jin-De
JD
D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology; D001161:Arteriosclerosis / Q000209:etiology / Q000473:pathology; D002478:Cells, Cultured; D003920:Diabetes Mellitus / Q000378:metabolism; D005947:Glucose / Q000008:administration & dosage / Q000494:pharmacology*; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis* / Q000235:genetics; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D012333:RNA, Messenger; D020778:Matrix Metalloproteinase 2; D005947:Glucose