title: Construction and characterization of rhesus monkey rotavirus (MMU18006)- or bovine rotavirus (UK)-based serotype G5, G8, G9 or G10 single VP7 gene substitution reassortant candidate vaccines.
abstract: Group A rotaviruses are the single most important etiologic agents of severe diarrhea of infants and young children worldwide and have been estimated to be responsible for approximately 650,000-800,000 deaths annually in children <5-year-old in the developing countries. Thus, the development of a safe and effective rotavirus vaccine has been a global public health goal. Epidemiologic surveillance of rotavirus VP7 (G) serotypes-genotypes conducted in various populations throughout the world has repeatedly shown that approximately 90% of the typeable rotavirus isolates belong to G1-G4. For these reasons, we have developed a rhesus rotavirus (RRV)-based or bovine rotavirus (UK)-based quadrivalent vaccine which is designed to provide antigenic coverage for G1-G4. More recently, G serotypes-genotypes other than G1-G4, including G5, G8-G10, have been detected in various parts of the world. Although the occurrence of such uncommon G types, except for G9, has been focal, still, in order to "be ready and prepared", we have constructed and characterized eight additional reassortant rotavirus vaccines, each of which bears a single human or bovine VP7 gene encoding G serotype 5, 8, 9 or 10 specificity and the remaining 10 genes of RRV strain MMU18006 or bovine rotavirus strain UK. These candidate vaccines could be evaluated singly in special populations or in combination with a RRV- or an UK-based quadrivalent vaccine to broaden its G serotype specificity.
authors.affiliation: Epidemiology Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Building 50, Room 6308, 50 South Drive, MSC 8026, Bethesda, MD 20892-8026, USA. thoshino@niaid.nih.gov
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Hoshino
authors.forename: Ronald W
authors.initials: RW
authors.lastname: Jones
authors.forename: Jerri
authors.initials: J
authors.lastname: Ross
authors.forename: Albert Z
authors.initials: AZ
authors.lastname: Kapikian
mesh_terms: D000818:Animals; D000956:Antigens, Viral; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology*; D002417:Cattle; D002460:Cell Line; D002648:Child; D002522:Chlorocebus aethiops; D006801:Humans; D008253:Macaca mulatta; D016865:Reassortant Viruses / Q000235:genetics / Q000276:immunology*; D012401:Rotavirus / Q000145:classification / Q000235:genetics / Q000276:immunology*; D022243:Rotavirus Vaccines / Q000235:genetics* / Q000276:immunology*; D012703:Serotyping
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D022243:Rotavirus Vaccines; C050779:VP7 protein, Rotavirus; C052014:rhesus rotavirus vaccine