Characterization of the Manila family of Mycobacterium tuberculosis.
Forty-eight Mycobacterium tuberculosis strains were obtained from patients living in metropolitan Manila, Republic of the Philippines. Three molecular typing methods, IS6110 restriction fragment length polymorphism, spoligotyping, and DNA sequencing of the oxyR, gyrA, and katG loci, established that these strains have restricted diversity and are members of a related genetic group of organisms. Comparison of the DNA fingerprint patterns with those in international databases confirmed the uniqueness of this group of isolates, which we designate the Manila family of M. tuberculosis.
Douglas
James T
JT
Department of Microbiology, University of Hawaii, Honolulu, Hawaii 96822, USA. jdouglas@hawaii.edu
Qian
Lishi
L
Montoya
Jaime C
JC
Musser
James M
JM
Van Embden
Jan D A
JD
Van Soolingen
Dick
D
Kremer
Kristin
K
D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D016172:DNA Fingerprinting / Q000379:methods; D004251:DNA Transposable Elements; D016208:Databases, Factual; D006801:Humans; D007391:International Cooperation; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics*; D009841:Oligonucleotides / Q000032:analysis; D010679:Philippines / Q000453:epidemiology; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D014397:Tuberculosis, Pulmonary / Q000453:epidemiology* / Q000382:microbiology
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D009841:Oligonucleotides