Molecular cloning and structural analysis of the porcine homologue to CD97 antigen.
CD97 is a member of a novel subfamily of leukocyte proteins that are characterized by the presence of tandemly repeated extracellular epidermal growth factor (EGF)-like domains and a seven-span transmembrane region, known as EGF-TM7. We here report the cloning of cDNA encoding the pig homologue of CD97. A pig CD97 specific probe was generated by PCR amplification of pig leukocyte cDNA, using primers based on consensus regions among the known sequences of mouse and human CD97. Screening of a pig aorta smooth muscle cDNA library identified one clone containing an open reading frame (ORF) that encoded an 18 amino acid putative signal peptide, a 141 amino acid sequence consisting of three EGF domains, a mucin-like spacer region of 276 amino acid, containing a G-protein coupling motif of 52 amino acids, followed by a 250 amino acid region containing seven membrane spanning domains and a 47 amino acid cytoplasmic tail. The amino acid sequence of the clone was 75, 67 and 59% homologous to cattle, human and mouse CD97 antigen, respectively. Therefore, it was termed pig CD97. Pig CD97 antigen shares many structural features with human, cattle and mouse CD97. RT-PCR analysis of cDNA from different pig cells and tissues showed that CD97 was highly expressed in leukocytes and lymph node cells. This is the first report describing the identification of a member of the EGF-TM7 family in the pig.
de la Lastra
José M Pérez
JM
Departamento de Genética, Unidad Mixta Consejo Superior de Investigaciones Científicas-Universidad de Córdoba, Campus de Rabanales, Edificio C5, 14071 Córdoba, Spain. jlastra@unavarra.es
Shahein
Yasser E A
YE
Garrido
Juan J
JJ
Llanes
Diego
D
D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007668:Kidney / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008198:Lymph Nodes / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000737:chemistry* / Q000235:genetics*; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism; D010053:Ovary / Q000378:metabolism; D011487:Protein Conformation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled; D016415:Sequence Alignment; D013154:Spleen / Q000378:metabolism; D013552:Swine / Q000235:genetics*
C095004:ADGRE5 protein, human; C490633:Adgre5 protein, mouse; D015703:Antigens, CD; D018076:DNA, Complementary; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled