title: ATP-citrate lyase as a substrate of protein histidine phosphatase in vertebrates.
abstract: The first protein histidine phosphatase from vertebrates discovered recently was found in a variety of tissues, however, a physiological substrate protein was missing. Phosphorylation of liver extracts in the presence of EDTA, followed by SDS-PAGE and autoradiography showed labeling of three proteins. Acid- and alkaline-treatment revealed the existence of N-phosphates. Addition of histidine phosphatase exclusively resulted in dephosphorylation of a 110kDa protein (denaturing conditions). Gelfiltration revealed its native molecular mass of approximately 450kDa. That protein was purified and identified as ATP-citrate lyase. The results are in favor of histidine phosphatase playing an important yet unidentified role in metabolic processes.
authors.affiliation: Institut für Pharmazeutische and Medizinische Chemie, Westfälische Wilhelms-Universität, Hittorfstr. 58-62, D-48149, Münster, Germany. klumpp@uni-muenster.de
authors.forename: Susanne
authors.initials: S
authors.lastname: Klumpp
authors.forename: Gunther
authors.initials: G
authors.lastname: Bechmann
authors.forename: Anette
authors.initials: A
authors.lastname: Mäurer
authors.forename: Dagmar
authors.initials: D
authors.lastname: Selke
authors.forename: Josef
authors.initials: J
authors.lastname: Krieglstein
mesh_terms: D001275:ATP Citrate (pro-S)-Lyase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D006639:Histidine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D011817:Rabbits; D013379:Substrate Specificity
chemical_list: D006639:Histidine; D001275:ATP Citrate (pro-S)-Lyase; D010749:Phosphoprotein Phosphatases; C005436:phosphohistidine