Influence of different types of exercise on the expression of haem oxygenase-1 in leukocytes.
Haem-oxygenase-1 (HO-1) is an antioxidant stress protein that is mainly induced by reactive oxygen species, inflammatory cytokines and hyperthermia. We assessed the influence of different types of exercise on HO-1 expression in leukocytes of the peripheral blood in three groups of male participants: a short exhaustive run above the lactate steady state (n = 15), eccentric exercise (n = 12) and an intensive endurance run (half-marathon, n = 12). Blood samples were taken at rest and up to 24 h after exercise. Blood lactate concentration after exercise was 9.0 +/- 2.1, 3.8 +/- 1.6 and 5.1 +/- 2.2 mmol x l(-1) (mean +/- s) for the exhaustive run, eccentric exercise and half-marathon groups, respectively (P < 0.05). Creatine kinase concentration was highest 24 h after exercise: 133 +/- 91, 231 +/- 139 and 289 +/- 221 U x l(-1) for the exhaustive run, eccentric exercise and half-marathon groups, respectively (P < 0.05). The maximal increase in leukocyte counts after exercise was 11.5 +/- 19.2, 6.2 +/- 1.4 and 14.7 +/- 2.1 x 10(9) x l(-1). There was no change in HO-1 as a result of the short exhaustive run or the eccentric exercise, whereas the half-marathon had a significant stimulatory effect on HO-1-expression in lymphocytes, monocytes and granulocytes (P < 0.001) using flow cytometry analyses. In conclusion, eccentric exercise alone or short-term heavy exercise are not sufficient to stimulate the antioxidative stress protein HO-1 in peripheral leukocytes
Fehrenbach
E
E
Department of Transfusion Medicine, University of Tübingen, Germany. elvira.fehrenbach@med.uni-tuebingen.de
Niess
A M
AM
Passek
F
F
Sorichter
S
S
Schwirtz
A
A
Berg
A
A
Dickhuth
H H
HH
Northoff
H
H
D000328:Adult; D003402:Creatine Kinase / Q000097:blood; D015444:Exercise / Q000502:physiology*; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism*; D051547:Heme Oxygenase-1; D006801:Humans; D019344:Lactic Acid / Q000097:blood; D007958:Leukocyte Count; D007962:Leukocytes / Q000201:enzymology*; D008297:Male; D008565:Membrane Proteins; D010807:Physical Endurance / Q000502:physiology; D012420:Running / Q000502:physiology
D008565:Membrane Proteins; D019344:Lactic Acid; C496541:HMOX1 protein, human; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1; D003402:Creatine Kinase