Application of a flow-type antibody sensor to the detection of Escherichia coli in various foods.
A flow-type biosensor system which uses a broad-spectrum anti-Escherichia coli antibody and quartz crystal microbalance as biological component and transducer was developed. Biosensor responses were initiated by injecting viable E. coli suspensions through a flow cell and the sensor system was optimized for response time according to flow rate and injection time, followed by the measurement of responses for various E. coli strains. As expected, the sensor system showed a characteristic broad binding feature against E. coli strains. A linear sensor response in double-logarithmic scale was observed for the microbial suspensions ranging from 1.7 x 10(5) to 8.7 x 10(7) CFU/ml. Sample measurements could be done within 20-30 min after Stomacher treatment followed by spiking or enrichment.
Kim
Namsoo
N
Korea Food Research Institute, San 46-1, Baekhyun-dong, Bundang-gu, Songnam-si, 463-420, Kyonggi-do, South Korea. k9130sen@hanmail.net
Park
In Seon
IS
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D019544:Equipment Failure Analysis; D004926:Escherichia coli / Q000276:immunology / Q000302:isolation & purification*; D017022:Flow Injection Analysis / Q000295:instrumentation* / Q000379:methods; D005504:Food Analysis / Q000295:instrumentation* / Q000379:methods; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D007124:Immunoenzyme Techniques / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014159:Transducers