Reactivity of new adhesion molecules on lymphocytes from patients with chronic graft versus host disease.
Reaction patterns of the 7th Human Leukocyte Differentiation Antigen Workshop blind panel adhesion molecules were studied on CD3/CD4, CD3/CD8, CD3/TCR gamma delta double positive T cells from peripheral blood of patients with chronic graft versus host disease (n = 8) and healthy controls (n = 4). Reactivity of 14 adhesion antibodies was tested by three-colour immunophenotyping. The mean proportion of CD3+ T cells (69 +/- 19%). CD3/CD8++ (31 +/- 13%) and CD3/TCR gamma delta++ (4 +/- 2%) T sub-populations of patients were comparable with the healthy controls. However, the mean percentage of CD3/CD4++ T cell subset in patients (14 +/- 12%) proved to be significantly decreased in comparison with the normal control value (34 +/- 16%) presumably due to secondary immunodeficiency. The workshop antibodies proved to be reactive with three T cell subsets expressing the examined antigens. Based on the results of the adhesion molecule workshop new CD categories have been introduced: CD156b as a transmembrane protein, CD167a as an epithelial tyrosin kinase receptor, CD168 as a receptor for hyaluronan mediated motility (RHAMM) and CD171 as a co-stimulatory adhesion molecule. There were significant differences in the expression of the CD167a and CD156b antigens on the CD3/CD4++ subset between the samples of patients compared with the controls characterizing the CD4+ T lymphocyte subpopulation in chronic graft versus host disease.
Regéczy
Nóra
N
National Medical Centre, Institute of Haematology and Immunology, Research Group of Hungarian Academy of Sciences, Budapest, Hungary.
Kormos
Luca
L
Szigetvári
Csilla M
CM
Torbágyi
Eva
E
Hajdu
Melinda
M
Gopcsa
L
L
Bányai
Anikó
A
Pálóczi
Katalin
K
D051722:ADAM Proteins; D000328:Adult; D015703:Antigens, CD / Q000378:metabolism*; D017252:CD3 Complex / Q000378:metabolism; D015704:CD4 Antigens / Q000378:metabolism; D016827:CD8 Antigens / Q000378:metabolism; D002908:Chronic Disease; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005260:Female; D006086:Graft vs Host Disease / Q000276:immunology*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D016130:Immunophenotyping; D008297:Male; D008565:Membrane Proteins; D008666:Metalloendopeptidases / Q000378:metabolism; D008875:Middle Aged; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism*; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D014184:Transplantation, Homologous
D015703:Antigens, CD; D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens; D016326:Extracellular Matrix Proteins; D018960:Hyaluronan Receptors; D008565:Membrane Proteins; D016692:Receptors, Antigen, T-Cell, gamma-delta; C098946:hyaluronan-mediated motility receptor; D051722:ADAM Proteins; C068490:ADAM8 protein, human; D008666:Metalloendopeptidases