title: Differential expression of two type-A response regulators in plants and cell cultures of Catharanthus roseus (L.) G. Don.
abstract: Two full-length cDNAs named CrRR2 and CrRR3 have been isolated by PCR from a C. roseus cDNA library. The first one encodes a 154 amino acid putative protein with a high percentage of identity with the Arabidopsis thaliana response regulators ARR16 and ARR17, and is not expressed in C. roseus organs and cell cultures. The second one encodes a 188 amino acid ORF sharing the highest homologies with the A. thaliana ARR8 and ARR9 response regulators. Its expression is root-specific and the transcripts are transiently up-regulated after trans-zeatin treatment in C. roseus suspension cells. CrRR3 protein might be involved in the cytokinin-enhanced alkaloid production in C. roseus cell cultures.
authors.affiliation: EA 2106, Biomolécules et Biotechnologies Végétales, Université de Tours, 31 Avenue Monge, F-37200 Tours, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Papon
authors.forename: Audrey
authors.initials: A
authors.lastname: Oudin
authors.forename: Amerin
authors.initials: A
authors.lastname: Vansiri
authors.forename: Marc
authors.initials: M
authors.lastname: Rideau
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Chénieux
authors.forename: Joël
authors.initials: J
authors.lastname: Crèche
mesh_terms: D000595:Amino Acid Sequence; D030008:Catharanthus / Q000166:cytology / Q000235:genetics* / Q000254:growth & development; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008969:Molecular Sequence Data; D010937:Plant Growth Regulators / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D015026:Zeatin / Q000494:pharmacology
chemical_list: D018076:DNA, Complementary; D010937:Plant Growth Regulators; D010940:Plant Proteins; D015026:Zeatin