Cloning and characterisation of a cDNA encoding Japanese flounder Paralichthys olivaceus IgD.
A cDNA containing the gene for Japanese flounder IgD consisted of 3240 bp encoding 998 amino acid residues. The amino acid sequence of the constant region of Japanese flounder IgD shares 38-80% identity with the sequences of previously reported teleost IgDs. The structure of the constant region of Japanese flounder IgD, which contains the micro1, delta1, delta2, delta3, delta4, delta5, delta6, delta7, and TM regions, is similar to the structures of the constant regions of the IgDs of channel catfish and Atlantic salmon. Southern blot hybridisation showed that the Japanese flounder IgD gene exists as a single locus. The Japanese flounder IgD gene was mainly detected in peripheral blood leucocytes (PBLs) and small amounts were detected in the spleen, head and trunk kidney, although IgM mRNA was detected in similar amounts in PBLs, the head kidney, and spleen. The copy number of IgM mRNA in Japanese flounder PBL was 56-fold higher than that of IgD.
Hirono
Ikuo
I
Laboratory of Genetics, Department of Aquatic Biosciences, Tokyo University of Fisheries, Konan 4-5-7, Minato, Tokyo 108-8477, Japan.
Nam
Bo-Hye
BH
Enomoto
Jyungo
J
Uchino
Kanako
K
Aoki
Takashi
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern / Q000662:veterinary; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry*; D005432:Flounder / Q000235:genetics / Q000276:immunology*; D015870:Gene Expression / Q000235:genetics; D007072:Immunoglobulin D / Q000737:chemistry / Q000235:genetics*; D007075:Immunoglobulin M / Q000235:genetics; D007668:Kidney / Q000276:immunology; D007962:Leukocytes / Q000276:immunology; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013154:Spleen / Q000276:immunology
D018076:DNA, Complementary; D007072:Immunoglobulin D; D007075:Immunoglobulin M; D012333:RNA, Messenger