Localization of putative neurotransmitters in the mantle and siphuncle of the mollusc Nautilus pompilius L. (Cephalopoda).
The neurotransmitter supply in the nerve endings of the mantle and the siphuncle, i.e. in organs that are responsible for the shell formation in the ectocholeate Nautilus pompilius, were investigated with electron microscopical, fluorescence-, immuno- and enzyme histochemical methods as well as with high pressure liquid chromatography (HPLC). Using antibodies against serotonin and the tetrapeptide FMRF-amide, positive reactions were demonstrated immunohistochemically within the terminal nerve fibres of the mantle and the vessels of the siphuncle. Enzyme histochemical proof of the presence of specific acetylcholinesterase yielded positive results in the muscle fibres of the mantle and siphuncle. Additionally, in the mantle, glyoxylic acid-induced fluorescence was shown within the nerve endings indicating catecholamines as neurotransmitters, whereas in the siphuncle such fluorescence did not appear. However, the HPLC-analyses showed that in the mantle and also in the siphuncle the content of dopamine is higher than that of noradrenaline whereas only traces of adrenaline occur in both organs suggesting dopamine as a putative neurotransmitter. Transmission electron microscopical examination of the nerve endings of both organs revealed that different types of vesicles were distinguished that could be considered as cholinergic, aminergic and peptidergic structures.
Westermann
Bettina
B
Institut für Allgemeine Zoologie und Enwicklungsbiologie, Justus-Liebig-Universität Giessen, Stephanstrasse 24, 35390 Giessen, Germany.
Beuerlein
Knut
K
Hempelmann
Gunter
G
Schipp
Rudolf
R
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D002395:Catecholamines / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004298:Dopamine / Q000378:metabolism; D019835:FMRFamide / Q000737:chemistry / Q000276:immunology; D006038:Glyoxylates / Q000494:pharmacology; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008974:Mollusca / Q000737:chemistry*; D009412:Nerve Fibers / Q000648:ultrastructure; D009420:Nervous System / Q000033:anatomy & histology / Q000201:enzymology / Q000378:metabolism*; D018377:Neurotransmitter Agents / Q000378:metabolism*; D012701:Serotonin / Q000737:chemistry / Q000276:immunology; D014018:Tissue Distribution
D002395:Catecholamines; D006038:Glyoxylates; D018377:Neurotransmitter Agents; D012701:Serotonin; D019835:FMRFamide; D000110:Acetylcholinesterase; C031150:glyoxylic acid; D004298:Dopamine