Selective repression of c-fos gene transcription in rat embryo fibroblasts transformed by oncogenes E1A and cHa-ras.
Transformation of REF cells by oncogenes E1A and cHa-ras leads to activation of AP-1 factor concomitantly with down-regulation of c-fos gene transcription. Here we addressed two issues: (i) how does transcription of Fos/Jun-regulated genes change in the cells lacking Fos-Jun heterodimers; (ii) to which extent HDAC-mediated chromatin reorganization does affect, apart from c-fos, transcription of some other early and late-response genes. To this end, we studied the kinetics of serum-stimulated transcription of c-fos, c-jun, fra-1, egr-1, and cyclinD1 genes, as well as the effects of sodium butyrate, an inhibitor of histone deacetylase activity, on transcription of these genes in normal REF cells and transformants E1A+ras.
Abramova
M V
MV
Institute of Cytology, Russian Academy of Sciences, 194064, St. Petersburg, Russia.
Kukushkin
A N
AN
Svetlikova
S B
SB
Pospelova
T V
TV
Pospelov
V A
VA
D017500:Adenovirus E1A Proteins / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D002843:Chromatin / Q000378:metabolism; D019281:Dimerization; D005347:Fibroblasts / Q000378:metabolism; D006655:Histone Deacetylases / Q000378:metabolism; D007700:Kinetics; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012978:Sodium Oxybate / Q000494:pharmacology; D014158:Transcription, Genetic; D018631:ras Proteins / Q000378:metabolism
D017500:Adenovirus E1A Proteins; D002843:Chromatin; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D012978:Sodium Oxybate; D006655:Histone Deacetylases; D018631:ras Proteins