title: Functional analysis of the 37 kDa inner envelope membrane polypeptide in chloroplast biogenesis using a Ds-tagged Arabidopsis pale-green mutant.
abstract: To study the functions of the nuclear genes involved in chloroplast development, we systematically analyzed albino and pale-green Arabidopsis thaliana mutants by using a two-component transposon system based on the Ac/Ds element of maize as a mutagen. One of the pale-green mutants, albino or pale green mutant 1 (designated as apg1), did not survive beyond the seedling stage, when germinated on soil. The chloroplasts of the apg1 plants contained decreased numbers of lamellae with reduced levels of chlorophyll. A gene encoding a 37 kDa polypeptide precursor of the chloroplast inner envelope membrane was disrupted by insertion of the Ds transposon in apg1. The 37 kDa protein had partial sequence similarity to the S-adenosylmethionine-dependent methyltransferase. The apg1 plants lacked plastoquinone (PQ), suggesting that the APG1 protein is involved in the methylation step of PQ biosynthesis, which is localized at the envelope membrane. Our results demonstrate the importance of the 37 kDa protein of the chloroplast inner envelope membrane for chloroplast development in Arabidopsis.
authors.affiliation: Plant Functional Genomics Group, RIKEN (Institute of Physical and Chemical Research) Genomic Sciences Center, 1-7-22 Tsurumi-ku, Yokohama 230-0045, Japan.
authors.forename: Reiko
authors.initials: R
authors.lastname: Motohashi
authors.forename: Takuya
authors.initials: T
authors.lastname: Ito
authors.forename: Masatomo
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Teruaki
authors.initials: T
authors.lastname: Taji
authors.forename: Noriko
authors.initials: N
authors.lastname: Nagata
authors.forename: Tadao
authors.initials: T
authors.lastname: Asami
authors.forename: Shigeo
authors.initials: S
authors.lastname: Yoshida
authors.forename: Kazuko
authors.initials: K
authors.lastname: Yamaguchi-Shinozaki
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shinozaki
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D002736:Chloroplasts / Q000378:metabolism*; D003001:Cloning, Molecular; D003116:Color; D005808:Genes, Recessive / Q000235:genetics; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009154:Mutation / Q000235:genetics*; D010641:Phenotype; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D018087:Plastids / Q000235:genetics / Q000378:metabolism; D010971:Plastoquinone / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: C478613:APG1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger; D010971:Plastoquinone