Synthesis, refolding and protective immune responses of a potential antigen for human Respiratory Syncytial Virus vaccine.
The design of new antigens with both high immunogenic and safety properties is of particular interest to vaccine against infectious diseases. In the present study, we describe the synthesis and the refolding of peptide G20 derived from the Human Respiratory Syncytial Virus (hRSV) G-protein. G20 (MEF G140-190 G144-158) is a peptide of 69 amino acids with two disulfide bridges, which comprises multiple protective B-cell epitopes. It was deleted of the T helper cell epitope 184-198 of the RSV G-protein, which was found to induce pulmonary pathology after RSV challenge in mice. Interestingly, we showed in the present study that G20 generated a highly protective antibody response against RSV challenge in Balb/c mice. Therefore, G20 represents a new potential antigen for an RSV vaccine.
Klinguer-Hamour
C
C
Centre d'Immunologie Pierre Fabre, 5 avenue Napoléon III, BP 497, F-74164 Saint-Julien-en-Genevois, France.
Bussat
M-C
MC
Plotnicky
H
H
Velin
D
D
Corvaïa
N
N
Nguyen
T
T
Beck
A
A
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000097:blood; D000956:Antigens, Viral / Q000737:chemistry* / Q000276:immunology; D002942:Circular Dichroism; D003545:Cysteine / Q000737:chemistry; D018985:Epitopes, B-Lymphocyte / Q000737:chemistry / Q000276:immunology; D018984:Epitopes, T-Lymphocyte / Q000737:chemistry; D015707:HN Protein / Q000737:chemistry / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D017510:Protein Folding; D022261:Respiratory Syncytial Virus Vaccines / Q000008:administration & dosage / Q000737:chemistry* / Q000276:immunology; D018113:Respiratory Syncytial Virus, Human; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology; D014759:Viral Envelope Proteins
D000914:Antibodies, Viral; D000956:Antigens, Viral; D018985:Epitopes, B-Lymphocyte; D018984:Epitopes, T-Lymphocyte; D015707:HN Protein; D010455:Peptides; D022261:Respiratory Syncytial Virus Vaccines; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; C116204:attachment protein G; C481479:hHSV peptide G20; D003545:Cysteine