Synthesis of HyBeacons and dual-labelled probes containing 2'-fluorescent groups for use in genetic analysis.
An FMOC-protected 2'-hydroxyethyl uridine phosphoramidite has been used to synthesise fluorescein-labelled HyBeacon probes and "FAM-ROX" dual-labelled fluorogenic oligonucleotides.
Dobson
Neil
N
Department of Chemistry, University of Southampton, Highfield, Southampton, UK SO17 1BJ.
McDowell
David G
DG
French
David J
DJ
Brown
Lynda J
LJ
Mellor
John M
JM
Brown
Tom
T
D004735:Energy Transfer; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D017422:Sequence Analysis, DNA
D005456:Fluorescent Dyes