Isolation and characterization of a carboxylic transport protein JEN1 and its promoter from Metarhizium anisopliae.
Based on the flanking sequence of T-DNA of a T-DNA insertion mutant of Beauveria bassiana, T12, the full length cDNA of carboxylic transport protein, designated MaJen1, was cloned from Metarhizium anisopliae. MaJen1 is 1,695 bp long and contained a 1,524 bp ORF which predicted a protein of 508 amino acid. The amino acid sequence of the gene showed 69% and 31% identity to the carboxylic transport protein of Neurospore crassa and Saccharomyces cerevisiae, respectively. The genome sequence, GMaJen1, was amplified by PCR, indicating that there were two introns in GMaJen1. Southern analysis indicated that GMaJen1 was present as a singl copy in Metarhizium anisopliae. The result of RT-PCR showed that expression of MaJen1 was induced by the cuticle of cockroach and repressed by glucose. A 1,626 bp upstream sequence of GMaJen1 was amplified by YADE method, which contained several putative binding domains of glucose repressor.
Fang
Wei-Guo
WG
Biotechnology Research Center Southwest Agricultural University, Chongqing 400716, China.
Zhang
Yong-Jun
YJ
Xiao
Yue-Hua
YH
Ma
Jin-Cheng
JC
Yang
Xing-Yong
XY
Pei
Yan
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002264:Carboxylic Acids / Q000378:metabolism*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D006999:Hypocreales / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D027501:Monocarboxylic Acid Transporters / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D002264:Carboxylic Acids; D018076:DNA, Complementary; D004271:DNA, Fungal; D005656:Fungal Proteins; D027501:Monocarboxylic Acid Transporters; D012331:RNA, Fungal