[Study on mechanisms of human gastric carcinoma cells apoptosis induced by genistein].
In order to study the apoptosic effect of genistein on human gastric carcinoma SGC-7901 cells, DAPI dyeing and DNA electrophoresis were used. Western Blot was used to detect the changes of the expression of p53 protein, Caspase-3 after SGC-7901 cells were exposed to genistein. The results showed that genistein induced cell apoptosis. Genistein also decreased the expression of mutant p53, and increased the protein expression of Caspase-3. These indicated that genistein could induce the apoptosis of gastric carcinoma cells. The inhibition of the expression of mutant p53 and enhance of the protein expression of Caspase-3 would be possible mechanism.
Song
Danfeng
D
Public Health School, Harbin Medical University, Harbin 150001, China.
Na
Xiaolin
X
Liu
Ying
Y
Chi
Xiaoxing
X
D000230:Adenocarcinoma / Q000473:pathology; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D020169:Caspases / Q000096:biosynthesis / Q000235:genetics; D019833:Genistein / Q000494:pharmacology*; D006801:Humans; D008856:Microscopy, Fluorescence; D013025:Glycine max / Q000737:chemistry; D013274:Stomach Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis / Q000235:genetics
D000970:Antineoplastic Agents; D016159:Tumor Suppressor Protein p53; D019833:Genistein; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases