Fusion to a carrier protein and a synthetic propeptide enhances E7 HPV-16 production and secretion in Lactococcus lactis.
An inducible system to improve and stabilize the production of an extremely labile protein (E7 antigen of human papillomavirus type 16) was developed in the food-grade bacterium Lactococcus lactis. A protein carrier, the staphylococcal nuclease Nuc, was fused either to N- or C-termini of E7 protein, and the resulting hybrid proteins were rescued from intracellular proteolysis but poorly secreted by L. lactis. A synthetic propeptide (LEISSTCDA) was then fused and significantly improved the secretion efficiency of the hybrid protein Nuc-E7 by L. lactis.
Bermúdez-Humarán
Luis G
LG
Laboratorio de Inmunología y Virología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, NL México.
Cortes-Perez
Naima G
NG
Le Loir
Yves
Y
Gruss
Alexandra
A
Rodriguez-Padilla
Cristina
C
Saucedo-Cardenas
Odila
O
Langella
Philippe
P
Montes de Oca-Luna
Roberto
R
D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D013294:Lactococcus lactis / Q000235:genetics* / Q000378:metabolism*; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis* / Q000235:genetics*; D050725:Papillomavirus E7 Proteins; D015202:Protein Engineering / Q000379:methods*; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism; D011786:Quality Control; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*
D002352:Carrier Proteins; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D011498:Protein Precursors; D011993:Recombinant Fusion Proteins; C059731:oncogene protein E7, Human papillomavirus type 16