title: Standardisation and establishment of a rabies ELISA test in European laboratories for assessing the efficacy of oral fox vaccination campaigns.
abstract: A simple and rapid enzyme linked immunosorbent assay (ELISA) to detect rabies antibodies in field fox sera has been standardised and established in several European laboratories. The same panels of 100 coded sera were investigated by four laboratories using this ELISA assay and reference serum neutralisation techniques (fluorescent antibody virus neutralisation (FAVN) test and rapid fluorescent focus inhibition test (RFFIT)). This indirect ELISA technique is highly correlated with conventional seroneutralisation test on cell culture. Results obtained with this ELISA test provide an almost perfect agreement between laboratories while the agreement of FAVN test and RFFIT results is substantial and very satisfactory. In view of this study, this simple and rapid ELISA test is a suitable tool for evaluating the sero-conversion rate in fox populations following oral rabies vaccination campaigns in European countries.
authors.affiliation: AFSSA Nancy, Laboratoire d'études et de recherches sur la rage et la pathologie des animaux sauvages, B.P. 9, 54220 Malzeville, France. f.cliquet@afssa.fr
authors.forename: Florence
authors.initials: F
authors.lastname: Cliquet
authors.forename: Thomas
authors.initials: T
authors.lastname: Müller
authors.forename: Franco
authors.initials: F
authors.lastname: Mutinelli
authors.forename: Sabrina
authors.initials: S
authors.lastname: Geronutti
authors.forename: Bernard
authors.initials: B
authors.lastname: Brochier
authors.forename: Thomas
authors.initials: T
authors.lastname: Selhorst
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Schereffer
authors.forename: Nicolas
authors.initials: N
authors.lastname: Krafft
authors.forename: Jeanette
authors.initials: J
authors.lastname: Burow
authors.forename: Astrid
authors.initials: A
authors.lastname: Schameitat
authors.forename: Hartmut
authors.initials: H
authors.lastname: Schlüter
authors.forename: Michel
authors.initials: M
authors.lastname: Aubert
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards; D005060:Europe; D005455:Fluorescent Antibody Technique; D005589:Foxes / Q000276:immunology*; D007753:Laboratories / Q000592:standards*; D009500:Neutralization Tests; D011818:Rabies / Q000276:immunology / Q000517:prevention & control* / Q000662:veterinary*; D011819:Rabies Vaccines / Q000008:administration & dosage / Q000276:immunology*; D012015:Reference Standards; D012680:Sensitivity and Specificity; D014611:Vaccination / Q000662:veterinary
chemical_list: D000914:Antibodies, Viral; D011819:Rabies Vaccines