title: Synthetic gene libraries: in search of the optimal diversity.
abstract: Directed evolution has proven to be an effective method for evolving proteins with desired properties. A key step is the creation of suitably diverse gene libraries. Two new methods for creating such libraries make sole use of synthesized oligonucleotides and allow researchers to tailor the diversity of a library with greater precision and create libraries with greater diversity than was previously possible. Such increased diversity appears to accelerate directed evolution.
authors.affiliation: Dept of Chemical and Biomolecular Engineering, Johns Hopkins University, 3400 N. Charles St, Baltimore, MD 21218, USA. oster@jhu.edu
authors.forename: Marc
authors.initials: M
authors.lastname: Ostermeier
mesh_terms: D003001:Cloning, Molecular / Q000379:methods; D019020:Directed Molecular Evolution / Q000379:methods*; D004815:Epidermal Growth Factor / Q000235:genetics; D015723:Gene Library; D005813:Genes, Synthetic; D005818:Genetic Engineering / Q000379:methods*; D014644:Genetic Variation; D019151:Peptide Library; D015202:Protein Engineering / Q000379:methods; D011506:Proteins / Q000235:genetics*; D011995:Recombination, Genetic; D020860:Subtilisin / Q000235:genetics
chemical_list: D019151:Peptide Library; D011506:Proteins; D004815:Epidermal Growth Factor; D020860:Subtilisin