Induction of chromatin damage and distribution of isochromatid breaks in human fibroblast cells exposed to heavy ions.
The frequency of chromatid breaks and the distribution of isochromatid breaks were measured in G2-phase normal human fibroblasts prematurely condensed a short time after exposure to low- or high-LET radiations. The average number of isochromatid breaks from a single particle traversal increased with increasing LET values, while the average number of chromatid-type breaks appeared to reach a plateau. The distribution of isochromatid breaks after high-LET iron particles exposure was overdispersed compared to gamma-rays, indicating that a single iron particle traversal through a cell nucleus can produce multiple isochromatid breaks.
Kawata
Tetsuya
T
Department of Radiology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan. tkawata@med.m.chiba-u.ac.jp
Ito
Hisao
H
Motoori
Ken
K
Ueda
Takuya
T
Shigematsu
Naoyuki
N
Furusawa
Yoshiya
Y
Durante
Marco
M
George
Kerry
K
Wu
Honglu
H
Cucinotta
Francis A
FA
D002842:Chromatids / Q000528:radiation effects; D002843:Chromatin / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts / Q000528:radiation effects*; D020450:Heavy Ions; D006801:Humans; D007501:Iron; D018499:Linear Energy Transfer
D002843:Chromatin; D007501:Iron