[Expression of different RASSF1 transcripts and its clinical significance in lung carcinoma].
OBJECTIVE
To evaluate the expression of three different RASSF1 transcripts and its clinical significance in lung carcinomas.


METHODS
The mRNA expression of RASSF1A, RASSF1B and RASSF1C was detected by RT-PCR in 51 human lung cancer tissues and 51 matched normal tissues.


RESULTS
1. The mRNA expression of three RASSF1 transcripts was detectable in all non-cancer tissues. However, high rate of expression loss of RASSF1A and RASSF1B existed in lung cancer tissues, which was 53.2% (2851) and 37.3% (19/51), respectively. RASSF1C was expressed in all of the tumor tissues. 2. Loss or abnormal down-regulation of RASSF1A was positively related with lymph node metastasis and TNM stage (P < 0.05) and 3. RASSF1B and RASSF1C mRNA expression was not correlated with TNM stage, histological type, differentiation grade or smoking index.


CONCLUSIONS
There is a significant expression difference among the three RASSF1 transcripts in lung carcinoma. RASSF1A, closely associated with lymph metastasis and TNM stage of lung carcinoma, should be a new tumor suppressor gene.
Shao
Kang
K
Laboratory of Thoracic Surgical Oncology, Cancer Institute (Hospital), Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100021, China.
He
Jie
J
Cheng
Bang-chang
BC
Xiao
Zhi-xiong
ZX
Zhang
De-chao
DC
Huang
Wei
W
Zhang
Cui-yan
CY
Zhou
Fang
F
Xiong
Mei-hua
MH
Tang
Huai-jing
HJ
Shi
Su-sheng
SS
Mu
Ba-dong
BD
D002872:Chromosome Deletion; D002893:Chromosomes, Human, Pair 3; D016147:Genes, Tumor Suppressor; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008207:Lymphatic Metastasis; D009367:Neoplasm Staging; D012333:RNA, Messenger / Q000032:analysis*; D025521:Tumor Suppressor Proteins / Q000235:genetics*
C411762:RASSF1 protein, human; D012333:RNA, Messenger; D025521:Tumor Suppressor Proteins