Breaking the degeneracy of the genetic code.
A mutant yeast phenylalanine transfer RNA (ytRNAPheAAA) containing a modified (AAA) anticodon was generated to explore the feasibility of breaking the degeneracy of the genetic code in Escherichia coli. By using an E. coli strain co-transformed with ytRNAPheAAA and a mutant yeast phenylalanyl-tRNA synthetase, we demonstrate efficient replacement of phenylalanine (Phe) by L-3-(2-naphthyl)alanine (Nal) at UUU, but not at UUC codons.
Kwon
Inchan
I
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Kirshenbaum
Kent
K
Tirrell
David A
DA
D000818:Animals; D000926:Anticodon; D003062:Codon; D004926:Escherichia coli / Q000235:genetics*; D005815:Genetic Code; D051379:Mice; D010649:Phenylalanine / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D012360:RNA, Transfer, Phe / Q000235:genetics*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*
D000926:Anticodon; D003062:Codon; D012360:RNA, Transfer, Phe; D010649:Phenylalanine; D013762:Tetrahydrofolate Dehydrogenase