[Experimental molecular design of recombinant vaccines].
A method was elaborated to construct combined artificial immunogens mimicking virus particles. The gist was exposing protein antigenic determinants of one virus on the particle surface and delivering plasmids with genes for antigenic proteins of another virus to specialized immune cells. Such immunogens were constructed and shown to induce biosynthesis of specific antibodies against HIV-1 and the tick-borne encephalitis virus. The level and duration of the humoral and cell responses were assayed.
Lebedev
L R
LR
Vector State Research Center of Virology and Biotechnology, Ministry of Health of the Russian Federation, Kol'tsovo, Novosibirsk Oblast, 633159 Russia. llr@ngs.ru
Goncharova
E P
EP
Sizov
A A
AA
Bulychev
L E
LE
Odegov
A M
AM
Ryzhikov
A B
AB
D016915:AIDS Vaccines / Q000235:genetics / Q000276:immunology; D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000378:metabolism; D000917:Antibody Formation; D015195:Drug Design; D004669:Encephalitis Viruses, Tick-Borne / Q000276:immunology; D000939:Epitopes / Q000235:genetics; D016655:HIV Core Protein p24 / Q000235:genetics / Q000276:immunology; D015497:HIV-1 / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D010957:Plasmids / Q000235:genetics; D014614:Vaccines, Synthetic / Q000235:genetics* / Q000276:immunology* / Q000494:pharmacology; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology
D016915:AIDS Vaccines; D000906:Antibodies; D000939:Epitopes; D016655:HIV Core Protein p24; D014614:Vaccines, Synthetic; D014764:Viral Proteins