Half-genome human immunodeficiency virus type 1 constructs for rapid production of reporter viruses.
The use of reporter constructs of HIV-1 molecular clones has been useful in studying the effects of infection on individual cells. A strategy of producing viral half-genome constructs containing reporter genes that can be used to produce recombinant complete infectious virions is described. This approach allows rapid generation of reporter and non-reporter virions from the same genetic construct, obviating the need to produce a new reporter genetic construct for each viral change to be studied. We provide an example of the utility of this system for studying the effects of Nef on infected cells.
Ali
Ayub
A
Division of Infectious Diseases, Department of Medicine and AIDS Institute, Los Angeles Medical Center, University of California, 37-121 CHS, 10833 LeConte Avenue, Los Angeles, CA 90095-1688, USA.
Jamieson
Beth D
BD
Yang
Otto O
OO
D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D005434:Flow Cytometry; D015693:Gene Products, nef / Q000235:genetics / Q000378:metabolism; D017930:Genes, Reporter / Q000235:genetics*; D016679:Genome, Viral; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D006801:Humans; D051379:Mice; D011995:Recombination, Genetic; D013997:Time Factors; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D014771:Virion / Q000378:metabolism* / Q000472:pathogenicity; D054311:nef Gene Products, Human Immunodeficiency Virus
D015693:Gene Products, nef; D014764:Viral Proteins; D054311:nef Gene Products, Human Immunodeficiency Virus