Identification of 9 novel transcripts and two RGSL genes within the hereditary prostate cancer region (HPC1) at 1q25.
We applied a systematic bioinformatics approach, followed by careful manual inspection and experimental validation to identify additional expressed sequences located at the Hereditary Prostate Cancer Region (HPC1) between D1S2818 and D1S1642 on chromosome 1q25. All transcripts already described for the 1q25 region were identified and we were able to define 11 additional expressed sequences within this region (three full-length cDNA clone sequences and eight ESTs), increasing the total number of gene count in this region by 38%. Five out of the 11 expressed sequences identified were shown to be expressed in prostate tissue and thus represent novel disease gene candidates for the HPC1 region. Here, we report a detailed characterization of these five novel disease gene candidates, their expression pattern in various tissues, their genomic organization and functional annotation. Two candidates (RGSL1 and RGSL2) correspond to novel members of the RGS family, which is involved in the regulation of G-protein signaling. RGSL1 and RGLS2 expression was detected by real-time polymerase chain reaction in normal prostate tissue, but could not be detected in prostate tumor cell lines, suggesting they might have a role in prostate cancer.
Silva
Ana Paula M
AP
Ludwig Institute for Cancer Research, Rua Antonio Prudente 109, 4th floor, 01509-010, São Paulo, SP, Brazil.
Salim
Anna Christina M
AC
Bulgarelli
Adriana
A
de Souza
Jorge Estefano S
JE
Osório
Elisson
E
Caballero
Otavia L
OL
Iseli
Christian
C
Stevenson
Brian J
BJ
Jongeneel
C Victor
CV
de Souza
Sandro J
SJ
Simpson
Andrew J G
AJ
Camargo
Anamaria A
AA
D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D030561:Databases, Nucleic Acid; D020224:Expressed Sequence Tags; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D011506:Proteins / Q000235:genetics*; D020710:RGS Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic / Q000235:genetics; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D011506:Proteins; D020710:RGS Proteins; C476052:RGSL1 protein, human