title: Putative hAPN receptor binding sites in SARS_CoV spike protein.
abstract: OBJECTIVE
  To obtain the information of ligand-receptor binding between the S protein of SARS-CoV and CD13, identify the possible interacting domains or motifs related to binding sites, and provide clues for studying the functions of SARS proteins and designing anti-SARS drugs and vaccines.
  
  
  METHODS
  On the basis of comparative genomics, the homology search, phylogenetic analyses, and multi-sequence alignment were used to predict CD13 related interacting domains and binding sites in the S protein of SARS-CoV. Molecular modeling and docking simulation methods were employed to address the interaction feature between CD13 and S protein of SARS-CoV in validating the bioinformatics predictions.
  
  
  RESULTS
  Possible binding sites in the SARS-CoV S protein to CD13 have been mapped out by using bioinformatics analysis tools. The binding for one protein-protein interaction pair (D757-R761 motif of the SARS-CoV S protein to P585-A653 domain of CD13) has been simulated by molecular modeling and docking simulation methods.
  
  
  CONCLUSIONS
  CD13 may be a possible receptor of the SARS-CoV S protein, which may be associated with the SARS infection. This study also provides a possible strategy for mapping the possible binding receptors of the proteins in a genome.
authors.affiliation: Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
authors.forename: Xiao-Jing
authors.initials: XJ
authors.lastname: Yu
authors.forename: Cheng
authors.initials: C
authors.lastname: Luo
authors.forename: Jian-Cheng
authors.initials: JC
authors.lastname: Lin
authors.forename: Pei
authors.initials: P
authors.lastname: Hao
authors.forename: You-Yu
authors.initials: YY
authors.lastname: He
authors.forename: Zong-Ming
authors.initials: ZM
authors.lastname: Guo
authors.forename: Lei
authors.initials: L
authors.lastname: Qin
authors.forename: Jiong
authors.initials: J
authors.lastname: Su
authors.forename: Bo-Shu
authors.initials: BS
authors.lastname: Liu
authors.forename: Yin
authors.initials: Y
authors.lastname: Huang
authors.forename: Peng
authors.initials: P
authors.lastname: Nan
authors.forename: Chuan-Song
authors.initials: CS
authors.lastname: Li
authors.forename: Bin
authors.initials: B
authors.lastname: Xiong
authors.forename: Xiao-Min
authors.initials: XM
authors.lastname: Luo
authors.forename: Guo-Ping
authors.initials: GP
authors.lastname: Zhao
authors.forename: Gang
authors.initials: G
authors.lastname: Pei
authors.forename: Kai-Xian
authors.initials: KX
authors.lastname: Chen
authors.forename: Xu
authors.initials: X
authors.lastname: Shen
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Shen
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Zou
authors.forename: Wei-Zhong
authors.initials: WZ
authors.lastname: He
authors.forename: Tie-Liu
authors.initials: TL
authors.lastname: Shi
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhong
authors.forename: Hua-Liang
authors.initials: HL
authors.lastname: Jiang
authors.forename: Yi-Xue
authors.initials: YX
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D018826:CD13 Antigens / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020134:Catalytic Domain; D019295:Computational Biology; D006801:Humans; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D011485:Protein Binding; D025941:Protein Interaction Mapping; D017434:Protein Structure, Tertiary; D045473:Severe acute respiratory syndrome-related coronavirus / Q000737:chemistry* / Q000235:genetics; D016415:Sequence Alignment; D045169:Severe Acute Respiratory Syndrome / Q000821:virology*; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D008562:Membrane Glycoproteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins; C578557:spike glycoprotein, SARS-CoV; C578553:spike protein, mouse hepatitis virus; D018826:CD13 Antigens