title: Isolation of high molecular weight DNA from soil for cloning into BAC vectors.
abstract: Isolation of high molecular weight DNA fragments from soil, in excess of 1 Mb, and of sufficient quality for cloning into an Escherichia coli-streptomycete artificial chromosome vector is described. The combination of indirect extraction of cells, using a nycodenz extraction technique, followed by lysis of biomass immobilised in agarose plugs, allowed fragments in excess of 1 Mb to be purified.
authors.affiliation: Department of Biological Sciences, University of Warwick, Gibbet Hill Rd, Coventry CV4 7AL, UK. aberry@bio.warwick.ac.uk
authors.forename: Andrew E
authors.initials: AE
authors.lastname: Berry
authors.forename: Claudia
authors.initials: C
authors.lastname: Chiocchini
authors.forename: Tina
authors.initials: T
authors.lastname: Selby
authors.forename: Margherita
authors.initials: M
authors.lastname: Sosio
authors.forename: Elizabeth M H
authors.initials: EM
authors.lastname: Wellington
mesh_terms: D001419:Bacteria / Q000235:genetics; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D005822:Genetic Vectors; D007558:Italy; D008970:Molecular Weight; D012988:Soil Microbiology; D014197:Trees
chemical_list: D004269:DNA, Bacterial