title: Characterization of vpr vector constructed from chimeric simian and human immunodeficiency virus.
abstract: Chimeric simian and human immunodeficiency viruses (SHIVs) are useful tool for investigating AIDS pathogenesis and for development of vaccine. We constructed a SHIV-vpr vector (designated as SHIV-3sj) by replacing vpr region with restriction enzyme sites. SHIV-3sj was designed to express inserted gene along with its viral replication. Five cytokine genes were inserted into SHIV-3sj, and ability of viral replication and expression of the inserted genes were examined. The short insert including RANTES and IL-5 resulted in the successful expression from SHIV-3sj, while the construct having longer genes including IL-2, IL-6 and IL-12p35 failed to become replication competent. These results suggest that the length of the insert is an important factor for the replication ability of SHIV-3sj vector.
authors.affiliation: Department of Veterinary Microbiology, Miyazaki University, Miyazaki, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Haga
authors.forename: Masashi
authors.initials: M
authors.lastname: Okoba
authors.forename: Nanase
authors.initials: N
authors.lastname: Yamazaki
authors.forename: Shino
authors.initials: S
authors.lastname: Kumabe
authors.forename: Yuya
authors.initials: Y
authors.lastname: Shimizu
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Goto
authors.forename: Takeo
authors.initials: T
authors.lastname: Kuwata
authors.forename: Iouri L
authors.initials: IL
authors.lastname: Kozyrev
authors.forename: Masanori
authors.initials: M
authors.lastname: Hayami
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Miura
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D018946:Chemokine CCL5 / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics*; D015870:Gene Expression; D016334:Genes, vpr / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics* / Q000502:physiology*; D015497:HIV-1 / Q000235:genetics* / Q000502:physiology; D006801:Humans; D018664:Interleukin-12 / Q000235:genetics; D007376:Interleukin-2 / Q000235:genetics; D015848:Interleukin-5 / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics; D007700:Kinetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D015302:Simian Immunodeficiency Virus / Q000235:genetics* / Q000502:physiology; D013601:T-Lymphocytes / Q000821:virology; D014779:Virus Replication / Q000235:genetics*
chemical_list: D018946:Chemokine CCL5; D004274:DNA, Recombinant; D007376:Interleukin-2; D015848:Interleukin-5; D015850:Interleukin-6; D018664:Interleukin-12