title: Ectopic serotonin accumulation and efflux in rat mesencephalic slices after prior tissue 'radiolabelling'.
abstract: Radiolabelling of brain tissue has long been used to facilitate detection of transmitter efflux, on the assumption that egress of tritiated monoamines reflects that of the endogenous transmitter. The present study tested the hypothesis that the application of exogenous serotonin (5-HT) to mesencephalic slices, in the manner used during a typical radiolabelling protocol, leads to efflux of 5-HT from physiologically inappropriate loci such as other non-serotonergic neurones. We used fast cyclic voltammetry (FCV) to determine the effect of tissue pre-incubation with 5-HT on electrically-stimulated 5-HT efflux and reuptake in rat mesencephalic slices. Seven subregions were studied, including the dorsal raphe nucleus (DRN), dorsomedial periaqueductal grey (PAGdm) and the oral part of the pontine reticular nucleus (PnO). In control slices (pre-incubated without 5-HT), stimulated 5-HT efflux was only detectable in DRN, PAGdm and occasionally in PnO. In slices incubated in 5-HT (100nM) for 30min, stimulated 5-HT efflux was detected in all seven subregions studied. In such slices, citalopram (75nM) increased efflux and reuptake t(1/2) in DRN to 201+/-21 and 487+/-117% of pre-drug values (P<0.05) but had no significant effect on either measure in PnO. The 5-HT1 autoreceptor agonist, 5-carboxamidotryptamine (5-CT, 100nM) decreased efflux in DRN by 54+/-6% (P<0.05), but was without effect (10+/-14%) in PnO. The present results show that pre-incubation in 5-HT allows stimulated 5-HT efflux from regions of the mesencephalon other than DRN and PAGdm. This stimulated 5-HT efflux is apparently not influenced by 5-HT transporters or 5-HT1 autoreceptors, suggesting that efflux is ectopic, an artefact of the pre-incubation process. In summary, incubation of rat mesencephalic tissue in 5-HT, in the manner of a typical radiolabelling protocol, results in stimulated 5-HT efflux from non-physiological sites. The results of such transmitter efflux studies should thus be interpreted with caution.
authors.affiliation: Anaesthetics Unit, Neurotransmission Laboratory, Academic Department of Anaesthesia and Intensive Care, Barts and The London School of Medicine and Dentistry, Alexandra Wing, Royal London Hospital, Whitechapel, London E1 1BB, UK.
authors.forename: Karen L
authors.initials: KL
authors.lastname: Blatchford
authors.forename: Daniel P
authors.initials: DP
authors.lastname: McLaughlin
authors.forename: Jonathan A
authors.initials: JA
authors.lastname: Stamford
mesh_terms: D000818:Animals; D015283:Citalopram / Q000494:pharmacology; D004558:Electric Stimulation / Q000379:methods; D004563:Electrochemistry / Q000379:methods; D066298:In Vitro Techniques; D007553:Isotope Labeling / Q000379:methods; D008297:Male; D008636:Mesencephalon / Q000033:anatomy & histology / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000031:analogs & derivatives* / Q000378:metabolism* / Q000494:pharmacology; D012702:Serotonin Antagonists / Q000494:pharmacology; D017366:Serotonin Receptor Agonists / Q000494:pharmacology; D013997:Time Factors; D014018:Tissue Distribution; D014316:Tritium / Q000378:metabolism
chemical_list: D012702:Serotonin Antagonists; D017366:Serotonin Receptor Agonists; D015283:Citalopram; D014316:Tritium; D012701:Serotonin; C047087:5-carboxamidotryptamine