Inhibitory effects of green tea catechins on the activity of human matrix metalloproteinase 7 (matrilysin).
Inhibitory effects of green tea catechins and their derivatives on the matrilysin-catalyzed hydrolysis of a synthetic substrate, (7-methoxycoumarin-4-yl)acetyl-L-Pro-L-Leu-Gly-L-Leu-[N(3)-(2,4-dinitrophenyl)-L-2,3-diamino-propionyl]-L-Ala-L-Arg-NH(2) [MOCAc-PLGL(Dpa)AR], were examined. The 10 catechins examined were classified into three groups according to their inhibition potency. Catechins with a galloyl group at the 3 position, including a major component of green tea catechin, (-)-epigallo-3-catechin gallate [(-)-EGCG], were the most potent inhibitors and inhibited matrilysin in a non-competitive manner with K(i) values of 0.47-1.65 micro M. The inhibitory potency of (-)-EGCG was not influenced by the presence of an inhibitor, ZnCl(2), suggesting that the inhibitions of matrilysin by (-)-EGCG and by ZnCl(2) might be independent of each other. The inhibitory effects of green tea catechins suggest that a high intake of green tea might be effective for the prevention of tumor metastasis and invasion in which matrilysin is concerned.
Oneda
Hiroshi
H
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
Shiihara
Misa
M
Inouye
Kuniyo
K
D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D002712:Chlorides / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D020128:Inhibitory Concentration 50; D020783:Matrix Metalloproteinase 7 / Q000378:metabolism; D061965:Matrix Metalloproteinase Inhibitors; D013050:Spectrometry, Fluorescence; D013662:Tea / Q000737:chemistry*; D017967:Zinc Compounds / Q000494:pharmacology
D002712:Chlorides; D004791:Enzyme Inhibitors; D061965:Matrix Metalloproteinase Inhibitors; D013662:Tea; D017967:Zinc Compounds; C016837:zinc chloride; D002392:Catechin; C045651:epigallocatechin gallate; D020783:Matrix Metalloproteinase 7