Role of intrinsic nitrergic neurones on vagally mediated striated muscle contractions in the hamster oesophagus.
Oesophageal peristalsis is controlled by vagal motor neurones, and intrinsic neurones have been identified in the striated muscle oesophagus. However, the effect(s) of intrinsic neurones on vagally mediated contractions of oesophageal striated muscles has not been defined. The present study was designed to investigate the role of intrinsic neurones on vagally evoked contractions of oesophageal striated muscles, using hamster oesophageal strips maintained in an organ bath. Stimulation (30 micros, 20 V) of the vagus nerve trunk produced twitch contractions. Piperine inhibited vagally evoked contractions, while capsaicin and NG-nitro-L-arginine methyl ester (L-NAME) abolished the inhibitory effect of piperine. The effect of L-NAME was reversed by subsequent addition of L-arginine, but not by D-arginine. L-NAME did not have any effect on the vagally mediated contractions and presumed 3H-ACh release. NONOate, a nitric oxide donor, and dibutyryl cyclic GMP inhibited twitch contractions. Inhibition of vagally evoked contractions by piperine and NONOate was fully reversed by ODQ, an inhibitor of guanylate cyclase. Immunohistochemical staining showed immunoreactivity for nitric oxide synthase (NOS) in nerve cell bodies and fibres in the myenteric plexus and the presence of choline acetyltransferase and NOS in the motor endplates. Only a few NOS-immunoreactive portions in the myenteric plexus showed vanilloid receptor 1 (VR1) immunoreactivity. Our results suggest that there is a local neural reflex that involves capsaicin-sensitive neurones, nitrergic myenteric neurones and vagal motor neurones.
Izumi
Noriaki
N
Department of Pathogenetic Veterinary Science, United Graduate School, Gifu University, Yanagido 1-1, Gifu 501-1193, Japan.
Matsuyama
Hayato
H
Ko
Mifa
M
Shimizu
Yasutake
Y
Takewaki
Tadashi
T
D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D006224:Cricetinae; D003995:Dibutyryl Cyclic GMP / Q000494:pharmacology; D004558:Electric Stimulation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004947:Esophagus / Q000294:innervation* / Q000502:physiology*; D066298:In Vitro Techniques; D008297:Male; D008647:Mesocricetus; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology*; D018482:Muscle, Skeletal / Q000378:metabolism / Q000502:physiology*; D026602:Nitrergic Neurons / Q000502:physiology*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000378:metabolism; D011955:Receptors, Drug / Q000378:metabolism; D014630:Vagus Nerve / Q000502:physiology*
D004791:Enzyme Inhibitors; D020030:Nitric Oxide Donors; D011955:Receptors, Drug; D003995:Dibutyryl Cyclic GMP; D019001:Nitric Oxide Synthase; D002795:Choline O-Acetyltransferase; D000109:Acetylcholine