Purification of anthocyanins from species of Banksia and Acacia using high-voltage paper electrophoresis.
A new method has been developed for the isolation and rapid identification of anthocyanins from two floricultural crops based on the use of high-voltage paper electrophoresis with bisulphite buffer. Using this method, anthocyanin pigments were successfully purified as their negatively charged bisulphite-addition compounds from crude extracts of plant tissue. In conjunction with liquid chromatography-electrospray mass spectrometry, the method enabled the anthocyanins from the flowers of two Banksia species and the leaves of two Acacia species to be identified. The Banksia flowers contained both cyanidin and peonidin-based pigments, while the Acacia leaves contained cyanidin and delphinidin derivatives.
Asenstorfer
Robert E
RE
School of Agriculture and Wine, The University of Adelaide, Waite Campus, PMB 1, Glen Osmond SA 5064, Australia.
Morgan
Anne L
AL
Hayasaka
Yoji
Y
Sedgley
Margaret
M
Jones
Graham P
GP
D000045:Acacia / Q000737:chemistry*; D000872:Anthocyanins / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D004590:Electrophoresis, Paper / Q000379:methods*; D035264:Flowers / Q000737:chemistry; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry; D039105:Proteaceae / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
D000872:Anthocyanins