title: [Recombinant Helicobacter pylori urease B subunit and its biological properties].
abstract: OBJECTIVE
  To perform genetic recombination of the urease B subunit (UreB) of Helicobacter pylori (Hp) and examine the biological properties of the recombinant protein.
  
  
  METHODS
  The gene fragment encoding Hp UreB was isolated clinically from Chinese subjects by means of PCR, and cloned subsequently into an expression vector pET-11C-UreB for the non-fusion protein expression in E.coli BL21 (DE3) strain.
  
  
  RESULTS
  The expression of recombinant UreB was achieved in E.coli BL21 with a relative molecular weight of approximately 62,000 at the expression ratio of 26%, and the first 15 amino acids of recombinant UreB were MKKISREYVSMYGP. The results of peptide mapping and amino acid compositional analysis were consistent with previous theoretical prediction, and enzyme-linked immunosorbent assay together with Western blotting indicated strong immunogenicity and reactivity of the recombinant protein in BalB/c mice, which were specifically recognized by polyclonal BalB/c mice anti-Hp sera or human sera infected with Hp.
  
  
  CONCLUSIONS
  The results of this study has laid an solid immunological foundation for incorporating recombinant UreB as a subunit vaccine component against Hp.
authors.affiliation: Teaching and Research Section of Clinical Microbiology and Immunology, Department of Medical Laboratory, Third Military Medical University, Chongqing 400038, China. mxh95xy@mail.tmmu.com.cn
authors.forename: Dong-shui
authors.initials: DS
authors.lastname: Lu
authors.forename: Xu-hu
authors.initials: XH
authors.lastname: Mao
authors.forename: Quan-ming
authors.initials: QM
authors.lastname: Zou
authors.forename: Chao
authors.initials: C
authors.lastname: Wu
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Wei-jun
authors.initials: WJ
authors.lastname: Zhang
authors.forename: Fu-kun
authors.initials: FK
authors.lastname: Wang
authors.forename: Qing-hua
authors.initials: QH
authors.lastname: Xie
authors.forename: Ping
authors.initials: P
authors.lastname: Luo
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D016480:Helicobacter pylori / Q000201:enzymology* / Q000276:immunology*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D010449:Peptide Mapping; D021122:Protein Subunits; D011994:Recombinant Proteins / Q000032:analysis / Q000276:immunology; D014510:Urease / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D022223:Vaccines, Subunit / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology*
chemical_list: D001428:Bacterial Vaccines; D021122:Protein Subunits; D011994:Recombinant Proteins; D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic; D014510:Urease