title: Studies on the interaction of the antibiotic moenomycin A with the enzyme penicillin-binding protein 1b.
abstract: The interaction of a moenomycin derivative with the enzyme penicillin binding protein 1b (PBP 1b) has been studied by means of STD NMR. The results obtained initiated the synthesis of a number of moenomycin derivatives modified in unit A including a moenomycin-ampicillin conjugate and determination of their antibiotic activities. A protocol is described that allows studying the interaction of moenomycin analogues with PBP 1b by fluorescence correlation spectroscopy.
authors.affiliation: Universität Leipzig, Fakultät für Chemie und Mineralogie, Johannisallee 29, D-04103 Leipzig, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Rühl
authors.forename: Mohammed
authors.initials: M
authors.lastname: Daghish
authors.forename: Andrij
authors.initials: A
authors.lastname: Buchynskyy
authors.forename: Karen
authors.initials: K
authors.lastname: Barche
authors.forename: Daniela
authors.initials: D
authors.lastname: Volke
authors.forename: Katherina
authors.initials: K
authors.lastname: Stembera
authors.forename: Uwe
authors.initials: U
authors.lastname: Kempin
authors.forename: Dietmar
authors.initials: D
authors.lastname: Knoll
authors.forename: Lothar
authors.initials: L
authors.lastname: Hennig
authors.forename: Matthias
authors.initials: M
authors.lastname: Findeisen
authors.forename: Ramona
authors.initials: R
authors.lastname: Oehme
authors.forename: Sabine
authors.initials: S
authors.lastname: Giesa
authors.forename: Juan
authors.initials: J
authors.lastname: Ayala
authors.forename: Peter
authors.initials: P
authors.lastname: Welzel
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors*; D001455:Bambermycins / Q000138:chemical synthesis / Q000494:pharmacology*; D002352:Carrier Proteins / Q000037:antagonists & inhibitors*; D004058:Diffusion; D006602:Hexosyltransferases / Q000037:antagonists & inhibitors*; D008826:Microbial Sensitivity Tests; D002267:Muramoylpentapeptide Carboxypeptidase / Q000037:antagonists & inhibitors*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D017830:Octoxynol; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases / Q000037:antagonists & inhibitors*; D011485:Protein Binding; D013050:Spectrometry, Fluorescence
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002352:Carrier Proteins; D046915:Penicillin-Binding Proteins; D001455:Bambermycins; C437243:moenomycin A; D017830:Octoxynol; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase