Muscle stem cells differentiate into haematopoietic lineages but retain myogenic potential.
Muscle-derived stem cells (MDSCs) can differentiate into multiple lineages, including haematopoietic lineages. However, it is unknown whether MDSCs preserve their myogenic potential after differentiation into other lineages. To address this issue, we isolated from dystrophic muscle a population of MDSCs that express stem-cell markers and can differentiate into various lineages. After systemic delivery of three MDSC clones into lethally irradiated mice, we found that differentiation of the donor cells into various lineages of the haematopoietic system resulted in repopulation of the recipients' bone marrow. Donor-derived bone-marrow cells, isolated from these recipients by fluorescence-activated cell sorting (FACS), also repopulated the bone marrow of secondary, lethally irradiated, recipients and differentiated into myogenic cells both in vitro and in vivo in normal mdx mice. These findings demonstrate that MDSC clones retain their myogenic potential after haematopoietic differentiation.
Cao
Baohong
B
Department of Orthopaedic Surgery and Molecular Genetics and Biochemistry, Children's Hospital and University of Pittsburgh, Pittsburgh, PA 15213, USA.
Zheng
Bo
B
Jankowski
Ron J
RJ
Kimura
Shigemi
S
Ikezawa
Makoto
M
Deasy
Bridget
B
Cummins
James
J
Epperly
Mike
M
Qu-Petersen
Zhuqing
Z
Huard
Johnny
J
D000818:Animals; D015415:Biomarkers; D001854:Bone Marrow Cells / Q000528:radiation effects; D002454:Cell Differentiation / Q000502:physiology*; D019070:Cell Lineage / Q000502:physiology*; D002478:Cells, Cultured; D002999:Clone Cells / Q000166:cytology* / Q000637:transplantation; D005434:Flow Cytometry; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000502:physiology; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D009124:Muscle Proteins / Q000378:metabolism; D018482:Muscle, Skeletal / Q000166:cytology / Q000196:embryology* / Q000254:growth & development*; D032446:Myoblasts / Q000166:cytology / Q000502:physiology; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology
D015415:Biomarkers; D009124:Muscle Proteins