title: A xyloglucan from seeds of the native Brazilian species Hymenaea courbaril for micropropagation of Marubakaido and Jonagored apples.
abstract: Xyloglucan was extracted from seeds of Hymenaea courbaril and mixed with agar to prepare a solid culture medium used for micropropagation of the Marubakaido apple rootstock (Malus prunifolia Borkh) and cv. Jonagored (Malus domestica). The performance on gels created from a blend of 0.4%agar and 0.2% xyloglucan (w/v) was compared with that on media gelled with a standard concentration 0.6% (w/v) of agar. The growth of shoots and the multiplication rate were higher on the modified culture medium than on the agar-gelled medium. The occurrence of hyperhydric shoots was lower on the modified medium. In the absence of auxin, shoot rooting reached 70% (Marubakaido) and 66% (Jonagored) on the agar-xyloglucan medium and 6.7% and 10.4%, respectively, on the agar medium. When 0.25 microM indole-3-butyric acid (IBA) was added to both media, the modified medium gave better results in terms of rooting percentage and quality of roots than the agar-gelled medium.
authors.affiliation: Quimica Industrial, Centro de Ciências Exatas e de Tecnologia, Pontifícia Universidade Católica do Paraná, Curitiba PR, Brazil.
authors.forename: N
authors.initials: N
authors.lastname: Lima-Nishimura
authors.forename: M
authors.initials: M
authors.lastname: Quoirin
authors.forename: Y G
authors.initials: YG
authors.lastname: Naddaf
authors.forename: H M
authors.initials: HM
authors.lastname: Wilhelm
authors.forename: L L F
authors.initials: LL
authors.lastname: Ribas
authors.forename: M-R
authors.initials: MR
authors.lastname: Sierakowski
mesh_terms: D000362:Agar / Q000494:pharmacology; D001938:Brazil; D046508:Culture Techniques / Q000379:methods; D004305:Dose-Response Relationship, Drug; D007887:Fabaceae / Q000737:chemistry*; D005782:Gels / Q000494:pharmacology; D005936:Glucans; D007211:Indoles / Q000494:pharmacology; D027845:Malus / Q000187:drug effects* / Q000254:growth & development; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development; D011134:Polysaccharides / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry*; D014990:Xylans
chemical_list: D005782:Gels; D005936:Glucans; D007211:Indoles; D010936:Plant Extracts; D011134:Polysaccharides; D014990:Xylans; C014612:indolebutyric acid; C029353:xyloglucan; D000362:Agar