Direct detection of potassium cations bound to G-quadruplex structures by solid-state 39K NMR at 19.6 T.
We report solid-state 39K NMR detection of the K+ ions bound to three G-quadruplex structures formed by self-assembly of 5'-tert-butyl-dimethylsilyl-2',3'-O-isopropylidene guanosine, guanosine, and guanosine 5'-monophosphate. The 39K NMR spectra clearly show different spectral signatures for K+ ions inside the G-quadruplex channel and for K+ ions bound to the phosphate groups. Solid-state 39K NMR spectra for hydrated K salts of adenosine 2'-monophosphate and adenosine 5'-diphosphate are also reported.
Wu
Gang
G
Department of Chemistry, Queen's University, Kingston, Ontario, Canada K7L 3N6. gangwu@chem.queensu.ca
Wong
Alan
A
Gan
Zhehong
Z
Davis
Jeffery T
JT
D002414:Cations, Monovalent; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D054856:G-Quadruplexes; D006151:Guanosine / Q000031:analogs & derivatives* / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D009690:Nucleic Acid Conformation; D011188:Potassium / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism
D002414:Cations, Monovalent; D006151:Guanosine; D004247:DNA; D011188:Potassium