title: Differential subcellular localization of phosphorylated neurofilament and tau proteins in degenerating neurons of the human entorhinal cortex.
abstract: A panel of novel monoclonal antibodies was tested on the human entorhinal cortex for the recognition of age- and disease-related changes of neurofilament proteins (NF). Several antibodies identified phosphorylated NF-H subunit, which occurred preferentially in those aged between 60 and 80 years and were localized in degenerating neurons. Such neurons also contained neurofibrillary tangles, but neurofilament aggregates did not co-localize with tangles, nor did the quantity nor the number of NF-positive neurons correlate with the severity of Alzheimer's disease. This points to a susceptibility of NF in a subset of neurons for phosphorylation- and metabolically related morphological changes during neurodegeneration.
authors.affiliation: Institut de Biologie Cellulaire et de Morphologie, Rue du Bugnon 9, 1005 Lausanne, Switzerland.
authors.forename: Raymonde
authors.initials: R
authors.lastname: Porchet
authors.forename: Alphonse
authors.initials: A
authors.lastname: Probst
authors.forename: Eda
authors.initials: E
authors.lastname: Dráberová
authors.forename: Pavel
authors.initials: P
authors.lastname: Dráber
authors.forename: Irène M
authors.initials: IM
authors.lastname: Riederer
authors.forename: Beat M
authors.initials: BM
authors.lastname: Riederer
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D018728:Entorhinal Cortex / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009410:Nerve Degeneration / Q000378:metabolism* / Q000473:pathology; D019636:Neurodegenerative Diseases / Q000378:metabolism; D016900:Neurofilament Proteins / Q000032:analysis / Q000378:metabolism*; D009474:Neurons / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D010766:Phosphorylation; D013347:Subcellular Fractions / Q000737:chemistry / Q000378:metabolism; D016875:tau Proteins / Q000032:analysis / Q000378:metabolism*
chemical_list: D016900:Neurofilament Proteins; D016875:tau Proteins