Plasma versus serum for detection of herpes simplex virus type 2-specific immunoglobulin G antibodies with a glycoprotein G2-based enzyme immunoassay.
To evaluate the consonance between plasma and serum for the detection of herpes simplex virus type 2-specific immunoglobulin G antibodies, we compared results from concurrently obtained plasma and sera in 710 sexually active women by using a glycoprotein G2-based enzyme-linked immunosorbent assay (Focus Technologies, Cypress, Calif.) and found 98.9% agreement between the two specimen types. Plasma appears to be an acceptable matrix for use with this assay.
Cherpes
Thomas L
TL
Department of Medicine, Division of Infectious Disease, Magee-Womens Research Institute, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA.
Meyn
Leslie A
LA
Hillier
Sharon L
SL
D000914:Antibodies, Viral / Q000097:blood*; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006558:Herpes Genitalis / Q000175:diagnosis*; D018258:Herpesvirus 2, Human / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D013048:Specimen Handling / Q000379:methods; D014759:Viral Envelope Proteins / Q000276:immunology
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D014759:Viral Envelope Proteins