Determination of antibacterial flomoxef in serum by capillary electrophoresis.
A determination method of flomoxef (FMOX) concentration in serum by capillary electrophoresis is developed. Serum samples are extracted with acetonitrile. After pretreatment, they are separated in a fused-silica capillary tube with a 25 mM borate buffer (pH 10.0) as a running buffer that contains 50mM sodium dodecyl sulfate. The FMOX and acetaminophen (internal standard) are detected by UV absorbance at 200 nm. Linearity (0-200 mg/L) is good, and the minimum limit of detection is 1.0 mg/L (S/N = 3). The relative standard deviations of intra- and interassay variability are 1.60-4.78% and 2.10-3.31%, respectively, and the recovery rate is 84-98%. This method can be used for determination of FMOX concentration in serum.
Kitahashi
Toshihiro
T
Department of Laboratory Medicine, Kinki University School of Medicine, 377-2 Ohno-higashi, Osakasayama, Osaka, Japan. kitahasi@med.kindai.ac.jp
Furuta
Itaru
I
D002511:Cephalosporins / Q000097:blood*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D002511:Cephalosporins; C045693:flomoxef