title: Characterization of Netrin-1, Neogenin and cUNC-5H3 expression during chick dorsal root ganglia development.
abstract: The molecular mechanisms that control the axon pathfinding of different subtypes of dorsal root ganglia (DRG) neurons are poorly understood. To address whether Netrin-1-Neogenin/UNC-5 signaling contributes to sensory axon pathfinding, we cloned chick UNC5 homolog 3 (cUNC-5H3) and characterized the spatial, cellular and temporal expression patterns of Netrin-1, Neogenin and cUNC-5H3 in cutaneous and proprioceptive DRG neurons. We have found that Netrin-1 is only expressed by late-arising cutaneous neurons. In contrast, cUNC-5H3 expression is restricted to proprioceptive neurons. Neogenin is expressed in all DRG neurons. Our results indicate that the pathfinding molecules, Netrin-1 and cUNC-5H3 are differentially expressed by early cutaneous and proprioceptive neurons.
authors.affiliation: Interdepartmental Program in Neuroscience, University of Utah, School of Medicine, 20 North, 1900 East, Salt Lake City, UT 84132-3401, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Guan
authors.forename: Maureen L
authors.initials: ML
authors.lastname: Condic
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002642:Chick Embryo; D005727:Ganglia, Spinal / Q000196:embryology* / Q000378:metabolism; D017403:In Situ Hybridization; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000096:biosynthesis / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D000075386:Netrin Receptors; D000075388:Netrin-1; D010802:Phylogeny; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D025521:Tumor Suppressor Proteins
chemical_list: D008565:Membrane Proteins; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D000075386:Netrin Receptors; C000617353:Ntn1 protein, mouse; D011956:Receptors, Cell Surface; D025521:Tumor Suppressor Proteins; C105653:Unc5c protein, mouse; C091279:neogenin; D000075388:Netrin-1