Dopamine inhibits cytokine release and expression of tyrosine kinases, Lck and Fyn in activated T cells.
The effect of dopamine (DA) on the release of cytokines from activated human T cells has been evaluated to analyze the mechanism by which physiological concentration of dopamine inhibits T cell proliferation. Dopamine inhibited anti-CD3 mAb-induced release of both Th1 and Th2 cytokines, IL2, IFN-gamma and IL4 from T cells by specific class of dopamine receptors. This action of dopamine was mediated by a new mechanism. Dopamine suppressed non-receptor tyrosine kinases, Lck and Fyn expression which are the initial and pivotal signaling steps in T cell receptor (TCR) mediated different down stream signaling cascades, leading to cytokine release and subsequent clonal expansion of these immune effector cells.
Ghosh
Manik Chandra
MC
Signal Transduction and Biogenic Amines Laboratory, Chittaranjan National Cancer Institute, Research Building, 37, SP Mukherjee Road, Calcutta 700026, India.
Mondal
Amal Chandra
AC
Basu
Sujit
S
Banerjee
Samir
S
Majumder
Jahar
J
Bhattacharya
Debasis
D
Dasgupta
Partha Sarathi
PS
D000328:Adult; D016207:Cytokines / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D004298:Dopamine / Q000494:pharmacology* / Q000502:physiology; D005260:Female; D006801:Humans; D015151:Immunoblotting; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D007376:Interleukin-2 / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D015847:Interleukin-4 / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D008213:Lymphocyte Activation; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck) / Q000037:antagonists & inhibitors* / Q000235:genetics; D008297:Male; D011233:Precipitin Tests; D011518:Proto-Oncogene Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics; D051076:Proto-Oncogene Proteins c-fyn; D017448:Receptors, Dopamine D2 / Q000096:biosynthesis / Q000235:genetics; D050637:Receptors, Dopamine D3; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D018417:Th1 Cells / Q000378:metabolism; D018418:Th2 Cells / Q000378:metabolism
D016207:Cytokines; C493593:DRD3 protein, human; D007376:Interleukin-2; D011518:Proto-Oncogene Proteins; D017448:Receptors, Dopamine D2; D050637:Receptors, Dopamine D3; D015847:Interleukin-4; D007371:Interferon-gamma; C494957:FYN protein, human; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D051076:Proto-Oncogene Proteins c-fyn; D004298:Dopamine