Disaccharide analysis of glycosaminoglycans synthesized by cardiac myxoma cells in tumor tissues and in cell culture.
We analyzed the main disaccharide units of glycosaminoglycans synthesized by cardiac myxoma cells in vivo and in cell culture using high-performance liquid chromatography after 1-phenyl-3-methyl-5-pyrasolone labeling. Cardiac myxoma tissues contained large amounts of chondroitin-6-sulfate (46%) and hyaluronic acid (32%), along with some chondroitin-4-sulfate (13%), chondroitin (6%), and much less dermatan sulfate (3%). Cultured cardiac myxoma cells synthesized mainly chondroitin-6-sulfate. The abundant glycosaminoglycans in myxoma tissues may make up the characteristic friable gelatinous matrix which is favorable for embolism and tumor cell growth.
Negishi
Mitsuko
M
Second Department of Internal Medicine, 3-39-22, Showa-machi, Maebashi, Gunma 371-8511, Japan.
Sakamoto
Hironosuke
H
Sakamaki
Tetsuo
T
Ishikawa
Osamu
O
Kanda
Tsugiyasu
T
Tamura
Jun-ichi
J
Kurabayashi
Masahiko
M
Nagai
Ryozo
R
D000328:Adult; D000368:Aged; D002809:Chondroitin Sulfates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003871:Dermatan Sulfate / Q000032:analysis*; D004187:Disaccharides / Q000032:analysis; D005260:Female; D006338:Heart Neoplasms / Q000737:chemistry* / Q000473:pathology; D006801:Humans; D006820:Hyaluronic Acid / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D009232:Myxoma / Q000737:chemistry* / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000378:metabolism
D004187:Disaccharides; D003871:Dermatan Sulfate; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates