title: [Preliminary study on effect of ganhuang injection on reject reaction of xenograft].
abstract: OBJECTIVE
  To study the effect of Ganhuang Injection (GHI) on reject reaction of xenograft.
  
  
  METHODS
  RT-PCR technique was used to detect the activated relevant gene expression in porcine endothelial cells (PEC), and 51Cr releasing method was used to test the killing and adhesion action of NK cells.
  
  
  RESULTS
  The normal human serum and human NK-92 cell could up-regulate the mRNA expressions of E-selectin and IL-1 alpha gene in PEC, showing the PEC activating action, GHI could inhibit these activated gene expressions. Cyto-toxic experiment showed that GHI could also inhibit the cytotoxicity of NK cell on PEC dose-dependently, which was in accord with its inhibition on adhesive action of NK on PEC.
  
  
  CONCLUSIONS
  GHI could inhibit not only the PEC activation in hyperacute rejection, but also the function of NK cells in delayed xenograft rejection.
authors.affiliation: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100080.
authors.forename: Bin
authors.initials: B
authors.lastname: Shi
authors.forename: Hui-jun
authors.initials: HJ
authors.lastname: Yin
authors.forename: Xiu-ying
authors.initials: XY
authors.lastname: Huang
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D019040:E-Selectin / Q000096:biosynthesis / Q000235:genetics; D004730:Endothelium, Vascular / Q000166:cytology; D006035:Glycyrrhiza / Q000737:chemistry*; D006084:Graft Rejection; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis / Q000235:genetics; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012250:Rheum / Q000737:chemistry*; D013552:Swine
chemical_list: D004365:Drugs, Chinese Herbal; D019040:E-Selectin; D007375:Interleukin-1; D012333:RNA, Messenger