title: Morphology and morphogenesis of severe acute respiratory syndrome (SARS)-associated virus.
abstract: After infecting the Vero E6 cells by nasal/throat swabs collected from SARS patients, we studied the SARS-associated virus by electron microscopy and molecular biological technique. The results show that the diameter of newly isolated virus is about 50 nm without envelope or 100 nm with envelope. The virus was proved to be a new coronavirus by RT-PCR and it responded positively to convalescent-phase serum specimen from SARS patients, which is the evidence that this new virus is etiologically linked to the outbreak of SARS. The morphogenesis and distribution of the virus are also discussed in this article.
authors.affiliation: State Key Lab for Biocontrol, Zhongshan University, Guangzhou 510275, China.
authors.forename: Qin-Fen
authors.initials: QF
authors.lastname: Zhang
authors.forename: Jin-Ming
authors.initials: JM
authors.lastname: Cui
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Huang
authors.forename: Wei
authors.initials: W
authors.lastname: Lin
authors.forename: Dong-Yan
authors.initials: DY
authors.lastname: Tan
authors.forename: Jie-Wei
authors.initials: JW
authors.lastname: Xu
authors.forename: Yi-Feng
authors.initials: YF
authors.lastname: Yang
authors.forename: Jing-Qiang
authors.initials: JQ
authors.lastname: Zhang
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Huan-Ying
authors.initials: HY
authors.lastname: Zheng
authors.forename: Qiu-Xia
authors.initials: QX
authors.lastname: Chen
authors.forename: Xin-Ge
authors.initials: XG
authors.lastname: Yan
authors.forename: Kui
authors.initials: K
authors.lastname: Zheng
authors.forename: Zhuo-Yue
authors.initials: ZY
authors.lastname: Wan
authors.forename: Jie-Cheng
authors.initials: JC
authors.lastname: Huang
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000821:virology; D002522:Chlorocebus aethiops; D006801:Humans; D007830:Larynx / Q000821:virology; D008854:Microscopy, Electron; D009305:Nasopharynx / Q000821:virology; D009685:Nuclear Envelope / Q000821:virology; D045473:Severe acute respiratory syndrome-related coronavirus / Q000276:immunology / Q000302:isolation & purification / Q000648:ultrastructure*; D045169:Severe Acute Respiratory Syndrome / Q000276:immunology / Q000821:virology*; D014709:Vero Cells / Q000821:virology; D014771:Virion / Q000648:ultrastructure