title: An overview of tests for animal tissues in feeds applied in response to public health concerns regarding bovine spongiform encephalopathy.
abstract: Enforcing the ban on meat-and-bone meal in feed for farmed animals, and especially ruminants, is considered an important measure to prevent the spread of bovine spongiform encephalopathy. The authors describe current analytical methods for the detection and identification of animal tissues in feed. In addition, recently approved requirements, such as the ban of intra-species recycling (practice of feeding an animal species with proteins derived from the bodies, or parts of bodies, of the same species) are described. In principle, four different approaches are currently applied, i.e. microscopic analysis, polymerase chain reaction, immunoassay analysis and near infrared spectroscopy or microscopy. The principal performance characteristics of these methods are presented and compared, and their specific advantages and disadvantages described. Special emphasis is also placed on the impact of rendering conditions, particularly high temperatures and on the use of molecular biology techniques.
authors.affiliation: European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Food and Feed Unit, Retieseweg, 2440 Geel, Belgium.
authors.forename: G
authors.initials: G
authors.lastname: Gizzi
authors.forename: L W D
authors.initials: LW
authors.lastname: van Raamsdonk
authors.forename: V
authors.initials: V
authors.lastname: Baeten
authors.forename: I
authors.initials: I
authors.lastname: Murray
authors.forename: G
authors.initials: G
authors.lastname: Berben
authors.forename: G
authors.initials: G
authors.lastname: Brambilla
authors.forename: C
authors.initials: C
authors.lastname: von Holst
mesh_terms: D000821:Animal Feed / Q000009:adverse effects / Q000032:analysis* / Q000592:standards; D000818:Animals; D001842:Bone and Bones; D002417:Cattle; D016643:Encephalopathy, Bovine Spongiform / Q000209:etiology / Q000517:prevention & control* / Q000635:transmission; D005062:European Union; D005506:Food Contamination / Q000331:legislation & jurisprudence / Q000517:prevention & control*; D005525:Food-Processing Industry / Q000331:legislation & jurisprudence / Q000379:methods / Q000592:standards*; D007150:Immunohistochemistry / Q000662:veterinary; D007881:Legislation, Food; D008460:Meat; D008853:Microscopy / Q000662:veterinary; D008967:Molecular Biology / Q000379:methods; D016133:Polymerase Chain Reaction / Q000662:veterinary; D019265:Spectroscopy, Near-Infrared / Q000662:veterinary