Effect of ionic medium on carbacholine-induced membrane depolarization in lumbricus terrestris somatic muscle cells.
Carbacholine depolarizes Lumbricus terrestris myocyte membrane. Addition of verapamil, tetrodotoxin, removal of K(+), Cl(-), and Ca(2+), and replacement of Ca(2+)with Mn(2+)in the bathing solution did not prevent the carbacholine-induced decrease in resting potential, but in a sodium-free medium carbacholine was ineffective. It was hypothesized that depolarization of the sarcolemma resulting from activation of cholinomimetic-sensitive channel-receptor complex of the Lumbricus terrestris somatic muscle cells is primarily determined by sodium permeability of the membrane.
Volkov
E M
EM
Kazan' State Medical University. emvolkov@kxn.ru
Nurullin
L F
LF
D000818:Animals; D002217:Carbachol / Q000494:pharmacology*; D003470:Culture Media; D066298:In Vitro Techniques; D007477:Ions / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects; D032342:Muscle Cells / Q000187:drug effects* / Q000378:metabolism*; D009835:Oligochaeta; D012508:Sarcolemma / Q000187:drug effects / Q000378:metabolism; D012964:Sodium / Q000378:metabolism / Q000494:pharmacology; D013779:Tetrodotoxin / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology
D003470:Culture Media; D007477:Ions; D013779:Tetrodotoxin; D002217:Carbachol; D012964:Sodium; D014700:Verapamil