title: Comparison of microscan broth microdilution, synergy quad plate agar dilution, and disk diffusion screening methods for detection of high-level aminoglycoside resistance in enterococcus species.
abstract: We compared the dried MicroScan microdilution panel, Synergy Quad plate agar dilution, and high-potency disk diffusion screening methods for the detection of high-level aminoglycoside resistance in 815 enterococcal bloodstream isolates. Agreement between the three methods was 99% when testing for high-level gentamicin resistance and 96% when testing for high-level streptomycin resistance.
authors.affiliation: Clinical Microbiology Laboratory, Duke University Medical Center, Durham, North Carolina 27710, USA. david.murdoch@cdhb.govt.nz
authors.forename: David R
authors.initials: DR
authors.lastname: Murdoch
authors.forename: Stanley
authors.initials: S
authors.lastname: Mirrett
authors.forename: Lizzie J
authors.initials: LJ
authors.lastname: Harrell
authors.forename: Susan M
authors.initials: SM
authors.lastname: Donabedian
authors.forename: Marcus J
authors.initials: MJ
authors.lastname: Zervos
authors.forename: L Barth
authors.initials: LB
authors.lastname: Reller
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016470:Bacteremia / Q000382:microbiology; D001769:Blood / Q000382:microbiology; D024881:Drug Resistance, Bacterial; D016983:Enterococcus / Q000187:drug effects*; D005839:Gentamicins / Q000494:pharmacology*; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D013307:Streptomycin / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins; D013307:Streptomycin