The role of growth factors on renal tubular cells submitted to hypoxia and deprived of glucose.
BACKGROUND
In acute renal failure (ARF) renal tubular cell death and detachment can be induced by necrotic and apoptotic mechanisms. Several studies have demonstrated some benefits of the use of growth factors in experimental models of ARF.


METHODS
MDCK cells were cultured in a glucose-free medium for 24h and were submitted to hypoxia (PO2 around 35 mmHg) for additional 24 h. To evaluate the possible protective role of growth factors, EGF, IGF-I or HGF were added to the medium (20 ng mL). LDH release, viability (acridine orange and ethidium bromide dyes) and quantification of apoptotic cells (Hoechst 33342 dye fluorescence) were determined.


RESULTS
In the injury group, an increase on LDH release (60% vs. 3%) and on number of apoptotic cells (22% vs. 0.2%) which was associated with a reduced cell viability (61% vs. 94%) when compared with controls. Only HGF, not EGF or IGF-I, was able to protect cells from injury. HGF caused a significant reduction on LDH release (30%) and on number of apoptotic cells (5%), with an increase on viability cellular (79%).


CONCLUSIONS
HGF decreases cell death on MDCK cells after hypoxic-induced injury, probably acting in both necrotic and apoptotic mechanisms.
de Souza Durão
Marcelino
M
Nephrology Division, Universidade Federal de São Paulo, Escola Paulista de Medicina, São Paulo, Brazil. m.durao@uol.com.br
Razvickas
Clara Versolato
CV
Gonçalves
Elsa Alídia Petry
EA
Okano
Iria Ruriko
IR
Camargo
Simone Mafalda Rodrigues
SM
Monte
Júlio Cesar Martins
JC
dos Santos
Oscar Fernando Pavão
OF
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015687:Cell Hypoxia / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004195:Disease Models, Animal; D004285:Dogs; D004815:Epidermal Growth Factor / Q000494:pharmacology / Q000502:physiology*; D005947:Glucose / Q000378:metabolism*; D017228:Hepatocyte Growth Factor / Q000494:pharmacology / Q000502:physiology*; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology / Q000502:physiology*; D007684:Kidney Tubules / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000187:drug effects / Q000378:metabolism
D004815:Epidermal Growth Factor; D017228:Hepatocyte Growth Factor; D007334:Insulin-Like Growth Factor I; D007770:L-Lactate Dehydrogenase; D005947:Glucose