title: Association of human kinesin superfamily protein member 4 with BRCA2-associated factor 35.
abstract: A large portion of human kinesin superfamily protein member 4 (KIF4) is associated with the nuclear matrix during the interphase, while a small portion is found in the cytoplasm. During mitosis, it is associated with chromosomes throughout the entire process. In the present study, we identified a protein that interacts with KIF4 using a yeast two-hybrid system, co-immunoprecipitation and co-fractionation. This protein is BRCA2-associated factor 35 (BRAF35) containing a non-specific DNA binding high-mobility-group domain and a kinesin-like coiled-coil domain. It appeared that the interaction between the two proteins occurs through their respective alpha-helical coiled-coil domains. The co-fractionation experiment revealed that KIF4 and BRAF35 were present in a complex of approx. 540 kDa. The composition and biological significance of this complex should be studied further.
authors.affiliation: Institute for Medical Sciences, School of Medicine, Ajou University, Suwon 442-749, South Korea.
authors.forename: Young Mi
authors.initials: YM
authors.lastname: Lee
authors.forename: Wankee
authors.initials: W
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D024682:BRCA2 Protein / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D015723:Gene Library; D005822:Genetic Vectors / Q000235:genetics; D006367:HeLa Cells; D006609:High Mobility Group Proteins; D006801:Humans; D016547:Kinesins / Q000737:chemistry / Q000378:metabolism*; D016350:Leucine Zippers; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics; D010455:Peptides / Q000378:metabolism; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014407:Tumor Cells, Cultured; D020798:Two-Hybrid System Techniques
chemical_list: D024682:BRCA2 Protein; D004268:DNA-Binding Proteins; C000625244:HMG20B protein, human; D006609:High Mobility Group Proteins; D009363:Neoplasm Proteins; D010455:Peptides; C484228:KIF4A protein, human; C484229:KIF4B protein, human; D016547:Kinesins