In vivo quantitative three-dimensional localization of tumor labeled with exogenous specific fluorescence markers.
We introduce a diffused optical detection system based on the administration of a fluorophore-antibody conjugate to diseased tissue. The conjugate interacts with the antigens expressed by the diseased tissue, resulting in fluorescent labeling of the antigen. By combining an optical detection system with a reconstruction algorithm developed on the basis of the random-walk model, we were able to determine the position of the fluorophore (and, thus, of the diseased cells) in the tissue. We present three-dimensional reconstructions of the location of a fluorophore (FITC-fluorescein isothiocyanate) in the tongues of mice. Measurements were performed with the fluorophore embedded at various simulated depths. The simulations were performed with agarose-based gel slabs applied to the tongue as tissuelike phantoms. Reconstructed fluorophore locations agree well with the actual values.
Gannot
Israel
I
Biomedical Engineering Department, Faculty of Engineering, Tel-Aviv University, Tel-Aviv 69978, Israel. gannot@eng.tau.ac.il
Garashi
Avital
A
Gannot
Gallya
G
Chernomordik
Victor
V
Gandjbakhche
Amir
A
D000465:Algorithms; D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D014062:Tongue Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured
D005456:Fluorescent Dyes; D016650:Fluorescein-5-isothiocyanate