title: Detection of Chlamydophila felis and feline herpesvirus by multiplex real-time PCR analysis.
abstract: Chlamydophila felis and feline herpesvirus (FHV) are pathogens commonly associated with feline respiratory and ocular disease. A real-time multiplex PCR assay was developed to allow detection of these organisms, together with feline 28S ribosomal DNA, in a single tube. Of 538 ocular swab samples tested, 123 were positive for FHV, 97 were positive for C. felis, and 16 were positive for both pathogens.
authors.affiliation: Division of Molecular and Cellular Biology, Department of Clinical Veterinary Science, University of Bristol, Langford, Bristol BS40 5DU, United Kingdom. c.r.helps@bristol.ac.uk
authors.forename: Chris
authors.initials: C
authors.lastname: Helps
authors.forename: Nicky
authors.initials: N
authors.lastname: Reeves
authors.forename: Kathy
authors.initials: K
authors.lastname: Egan
authors.forename: Pam
authors.initials: P
authors.lastname: Howard
authors.forename: Dave
authors.initials: D
authors.lastname: Harbour
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000382:microbiology* / Q000821:virology*; D002415:Cats; D002478:Cells, Cultured; D023501:Chlamydophila / Q000235:genetics / Q000302:isolation & purification*; D023521:Chlamydophila Infections / Q000382:microbiology / Q000662:veterinary; D003231:Conjunctivitis / Q000662:veterinary / Q000821:virology; D004275:DNA, Ribosomal / Q000032:analysis; D006564:Herpesviridae / Q000235:genetics / Q000302:isolation & purification*; D006566:Herpesviridae Infections / Q000662:veterinary / Q000821:virology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004275:DNA, Ribosomal; D012339:RNA, Ribosomal, 28S