title: Intestinal Oxalobacter formigenes colonization in calcium oxalate stone formers and its relation to urinary oxalate.
abstract: OBJECTIVE
  Oxalobacter formigenes is an anaerobic commensal colonic bacterium capable of degrading oxalate through the enzyme oxalyl-CoA decarboxylase. It has been theorized that individuals who lack this bacterium have higher intestinal oxalate absorption, leading to a higher urinary oxalate concentration and an increased risk of calcium oxalate urolithiasis. We performed a prospective, controlled study to evaluate O. formigenes colonization in calcium oxalate stone formers and to correlate colonization with urinary oxalate and other standard urinary stone risk factors.
  
  
  METHODS
  Thirty-five first-time calcium oxalate stone formers were compared with 10 control subjects having no history of urolithiasis and a normal renal ultrasound scan. All subjects underwent standard metabolic testing by submitting serum and 24-hour urine specimens. In addition, all subjects submitted stool samples for culture and detection of O. formigenes by Xentr(ix) O. formigenes Monitor.
  
  
  RESULTS
  Intestinal Oxalobacter was detected in only 26% of the stone formers compared with 60% of the controls (p < 0.05). Overall, the average urinary oxalate excretion by the two groups was similar (38.6 mg/day v 40.8 mg/day). Among stone formers, however, there were statistically higher urinary oxalate concentrations in O. formigenes-negative patients compared with those testing positive (41.7 mg/day v 29.4 mg/day) (p = 0.03). Furthermore, all 10 stone formers with hyperoxaluria (>44 mg/day) tested negative for O. formigenes (p < 0.05).
  
  
  CONCLUSIONS
  Calcium oxalate stone formers have a low rate of colonization with O. formigenes. Among stone formers, absence of intestinal Oxalobacter correlates with higher urinary oxalate concentration and an increased risk of hyperoxaluria. Introduction of the Oxalobacter bacterium or an analog of its enzyme oxalyl-CoA decarboxylase into the intestinal tract may be a treatment for calcium oxalate stone disease.
authors.affiliation: Department of Urology, University of California at Davis, Sacramento, California 95817, USA.
authors.forename: Scott A
authors.initials: SA
authors.lastname: Troxel
authors.forename: Harmeet
authors.initials: H
authors.lastname: Sidhu
authors.forename: Poonam
authors.initials: P
authors.lastname: Kaul
authors.forename: Roger K
authors.initials: RK
authors.lastname: Low
mesh_terms: D000328:Adult; D000368:Aged; D002129:Calcium Oxalate; D005243:Feces / Q000382:microbiology; D005260:Female; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D010070:Oxalates / Q000652:urine*; D020624:Oxalobacter formigenes / Q000302:isolation & purification*; D014545:Urinary Calculi / Q000737:chemistry / Q000382:microbiology* / Q000652:urine
chemical_list: D010070:Oxalates; D002129:Calcium Oxalate