Induction of an interferon response by RNAi vectors in mammalian cells.
DNA vectors that express short hairpin RNAs (shRNAs) from RNA polymerase III (Pol III) promoters are a promising new tool to reduce gene expression in mammalian cells. shRNAs are processed to small interfering RNAs (siRNAs) of 21 nucleotides (nt) that guide the cleavage of the cognate mRNA by the RNA-induced silencing complex. Although siRNAs are thought to be too short to induce interferon expression, we report here that a substantial number of shRNA vectors can trigger an interferon response.
Bridge
Alan J
AJ
Swiss Institute for Experimental Cancer Research, CH-1066 Epalinges, Switzerland.
Pebernard
Stephanie
S
Ducraux
Annick
A
Nicoulaz
Anne-Laure
AL
Iggo
Richard
R
D015088:2',5'-Oligoadenylate Synthetase / Q000378:metabolism*; D004790:Enzyme Induction; D005347:Fibroblasts / Q000378:metabolism; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D007372:Interferons / Q000378:metabolism*; D016086:Lentivirus / Q000235:genetics*; D008168:Lung / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012313:RNA / Q000737:chemistry* / Q000378:metabolism*; D012320:RNA Polymerase III / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism*; D014157:Transcription Factors / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism*; D014158:Transcription, Genetic; D014162:Transfection
C109882:MORF4 protein, human; D034741:RNA, Small Interfering; D014157:Transcription Factors; D012313:RNA; D007372:Interferons; C475085:OAS1 protein, human; D012320:RNA Polymerase III; D015088:2',5'-Oligoadenylate Synthetase