Regulated CPEB phosphorylation during meiotic progression suggests a mechanism for temporal control of maternal mRNA translation.
CPEB is an mRNA-binding protein that stimulates polyadenylation-induced translation of maternal mRNA once it is phosphorylated on Ser 174 or Thr 171 (species-dependent). Disruption of the CPEB gene in mice causes an arrest of oogenesis at embryonic day 16.5 (E16.5), when most oocytes are in pachytene of prophase I. Here, we show that CPEB undergoes Thr 171 phosphorylation at E16.5, but dephosphorylation at the E18.5, when most oocytes are entering diplotene. Although phosphorylation is mediated by the kinase aurora, the dephosphorylation is due to the phosphatase PP1. The temporal control of CPEB phosphorylation suggests a mechanism in which CPE-containing mRNA translation is stimulated at pachytene and metaphase I.
Tay
Joyce
J
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA.
Hodgman
Rebecca
R
Sarkissian
Madathia
M
Richter
Joel D
JD
D000818:Animals; D000918:Antibody Specificity; D064127:Aurora Kinases; D005260:Female; D018392:Genomic Imprinting; D008540:Meiosis / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D009154:Mutation; D009865:Oocytes / Q000378:metabolism / Q000502:physiology; D010053:Ovary / Q000196:embryology* / Q000378:metabolism*; D010749:Phosphoprotein Phosphatases / Q000378:metabolism; D010766:Phosphorylation; D026723:Polyadenylation; D011418:Prophase / Q000502:physiology; D014176:Protein Biosynthesis; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D013912:Threonine / Q000378:metabolism; D014981:Xenopus; D014157:Transcription Factors; D039221:mRNA Cleavage and Polyadenylation Factors
D064127:Aurora Kinases; D010749:Phosphoprotein Phosphatases; D017346:Protein Serine-Threonine Kinases; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D013912:Threonine; C490387:Cpeb1 protein, mouse; D014157:Transcription Factors; D039221:mRNA Cleavage and Polyadenylation Factors