title: Comparison of washing and swabbing procedures for collecting genital fluids to assess cervicovaginal shedding of herpes simplex virus type 2 DNA.
abstract: Asymptomatic genital shedding of herpes simplex virus type 2 (HSV-2) DNA was evidenced by real-time PCR in 25 (13.2%) of 188 cervicovaginal lavage samples and in only 13 (6.9%) paired cervicovaginal samples from 188 HSV-2-seropositive, nonpregnant childbearing-aged human immunodeficiency virus-seronegative women living in Gabon. These observations demonstrate that cervicovaginal washing is more suitable than endocervicovaginal swabbing for detecting and quantifying HSV-2 DNA by PCR in female genital secretions.
authors.affiliation: Laboratoire de Virologie, Département de Microbiologie, Université des Sciences de la Santé Maternité Joséphine Bongo, Gabon.
authors.forename: Angélique
authors.initials: A
authors.lastname: Ndjoyi-Mbiguino
authors.forename: Francis
authors.initials: F
authors.lastname: Ozouaki
authors.forename: Jérôme
authors.initials: J
authors.lastname: Legoff
authors.forename: François-Xavier
authors.initials: FX
authors.lastname: Mbopi-Kéou
authors.forename: Ali
authors.initials: A
authors.lastname: Si-Mohamed
authors.forename: Isabelle Ndombi
authors.initials: IN
authors.lastname: Onas
authors.forename: Evelyne
authors.initials: E
authors.lastname: Avoune
authors.forename: Laurent
authors.initials: L
authors.lastname: Bélec
mesh_terms: D000293:Adolescent; D000328:Adult; D001826:Body Fluids / Q000821:virology; D002584:Cervix Uteri / Q000821:virology; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006558:Herpes Genitalis / Q000821:virology*; D018258:Herpesvirus 2, Human / Q000302:isolation & purification*; D006801:Humans; D016133:Polymerase Chain Reaction; D013048:Specimen Handling / Q000379:methods*; D014621:Vagina / Q000821:virology; D017201:Virus Shedding
chemical_list: D004279:DNA, Viral