title: Identification of NDRG1 as an early inducible gene during in vitro maturation of cultured mast cells.
abstract: Coculture of mouse bone marrow-derived mast cells (BMMC) with fibroblasts in the presence of stem cell factor (SCF) facilitates morphological and functional maturation toward a connective tissue mast cell (CTMC)-like phenotype. By means of cDNA subtraction, we identified several inducible genes during this mast cell maturation process. Of approximately 100 sequenced clones induced, nearly 50% were chromosome 14-associated serine proteases. Approximately 14% encoded NDRG1, a 43-kDa cytosolic protein that has been implicated in cell differentiation. NDRG1 was distributed in the cytosol of cultured mast cells and CTMC in rat skin. Overexpression of NDRG1 in RBL-2H3 cells resulted in enhanced degranulation in response to various stimuli. Thus, NDRG1 may be a mast cell maturation-associated inducible protein that allows the cells to be susceptible to extracellular stimuli leading to degranulation. Additionally, several unique maturation-associated inducible genes were identified, molecular and functional characterization of which will provide new insights into mast cell biology.
authors.affiliation: Department of Health Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, 142-8555, Tokyo, Japan.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Taketomi
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Sugiki
authors.forename: Takanori
authors.initials: T
authors.lastname: Saito
authors.forename: Shin ichi
authors.initials: Si
authors.lastname: Ishii
authors.forename: Masato
authors.initials: M
authors.lastname: Hisada
authors.forename: Tamiko
authors.initials: T
authors.lastname: Suzuki-Nishimura
authors.forename: Masaatsu K
authors.initials: MK
authors.lastname: Uchida
authors.forename: Tae Chul
authors.initials: TC
authors.lastname: Moon
authors.forename: Hyeun Wook
authors.initials: HW
authors.lastname: Chang
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Natori
authors.forename: Shinobu
authors.initials: S
authors.lastname: Miyazawa
authors.forename: Rei
authors.initials: R
authors.lastname: Kikuchi-Yanoshita
authors.forename: Makoto
authors.initials: M
authors.lastname: Murakami
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kudo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018797:Cell Cycle Proteins / Q000096:biosynthesis* / Q000235:genetics*; D002460:Cell Line; D002478:Cells, Cultured; D018920:Coculture Techniques; D003600:Cytosol / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000378:metabolism; D007150:Immunohistochemistry; D007249:Inflammation; D007313:Insecta; D047908:Intracellular Signaling Peptides and Proteins; D008024:Ligands; D008407:Mast Cells / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D018613:Microscopy, Confocal; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D016133:Polymerase Chain Reaction; D017434:Protein Structure, Tertiary; D051381:Rats; D014162:Transfection; D000099259:N-myc Downstream-Regulated Gene 1 Protein
chemical_list: D018797:Cell Cycle Proteins; D018076:DNA, Complementary; D047908:Intracellular Signaling Peptides and Proteins; D008024:Ligands; D000099259:N-myc Downstream-Regulated Gene 1 Protein