The metabolic pathway of salicylic acid rather than of chlorogenic acid is involved in the stress-induced flowering of Pharbitis nil.
We examined the involvement of chlorogenic acid (CGA) and salicylic acid (SA) in the stress-induced flowering of Pharbitis nil (synonym Ipomoea nil). The incorporation efficiency of exogenously applied CGA and the deactivation rate of incorporated CGA were determined in cotyledons by high-performance liquid chromatography. The assay plants could not incorporate a sufficient amount of CGA via roots. The perfusion technique by which the assay solution was forced into the plant from the cut end of the hypocotyl improved the efficiency of CGA incorporation. However, no flower-inducing activity was detected, indicating that CGA was not involved in flowering. It was concluded that the close correlation between CGA content and flowering response is merely coincidence or a parallelism. Flowering under long-day conditions induced by low-temperature stress was completely inhibited by aminooxyacetic acid (AOA), an inhibitor of phenylalanine ammonialyase. The flower-inhibiting effect of AOA was nullified by co-applied t-cinnamic acid and by benzoic acid. This indicates that the metabolic pathway from t-cinnamic acid to SA via benzoic acid is involved in the stress-induced flowering. The results indicate that the metabolic pathway of SA is involved in the stress-induced flowering of P. nil not the metabolic pathway of CGA.
Hatayama
Tomomi
T
Department of Biology, Faculty of Science, Niigata University, 8050 Ikarashi 2, Niigata 950-2181, Japan.
Takeno
Kiyotoshi
K
D000625:Aminooxyacetic Acid / Q000494:pharmacology; D019817:Benzoic Acid / Q000494:pharmacology; D002726:Chlorogenic Acid / Q000378:metabolism* / Q000494:pharmacology; D002934:Cinnamates / Q000494:pharmacology; D003080:Cold Temperature; D035264:Flowers / Q000187:drug effects / Q000378:metabolism*; D027741:Ipomoea / Q000187:drug effects / Q000378:metabolism*; D008027:Light; D015394:Molecular Structure; D010935:Plant Diseases; D018517:Plant Roots; D020156:Salicylic Acid / Q000378:metabolism* / Q000494:pharmacology
D002934:Cinnamates; D000625:Aminooxyacetic Acid; D002726:Chlorogenic Acid; D019817:Benzoic Acid; D020156:Salicylic Acid