The PEF family proteins sorcin and grancalcin interact in vivo and in vitro.
The penta-EF hand (PEF) family of calcium binding proteins includes grancalcin, peflin, sorcin, calpain large and small subunits as well as ALG-2. Systematic testing of the heterodimerization abilities of the PEF proteins using the yeast two-hybrid and glutathione S-transferase pull-down assays revealed the new finding that grancalcin interacts strongly with sorcin. In addition, sorcin and grancalcin can be co-immunoprecipitated from lysates of human umbilical vein endothelial cells. Our results indicate that heterodimerization, in addition to differential interactions with target proteins, might be a way to regulate and fine tune processes mediated by calcium binding proteins of the penta-EF hand type.
Hansen
Christian
C
Institute of Molecular Biology, Department of Molecular Cell Biology, University of Copenhagen, Oester Farimagsgade 2A, Copenhagen, Denmark.
Tarabykina
Svetlana
S
la Cour
Jonas M
JM
Lollike
Karsten
K
Berchtold
Martin W
MW
D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D019281:Dimerization; D020832:EF Hand Motifs; D004730:Endothelium, Vascular / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D011233:Precipitin Tests; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D020798:Two-Hybrid System Techniques; D014471:Umbilical Veins / Q000166:cytology
D002135:Calcium-Binding Proteins; C503121:GCA protein, human; D011993:Recombinant Fusion Proteins; C503113:SRI protein, human; D005982:Glutathione Transferase