title: [Relationship between aquaporin-4 expression in astrocytes and brain edema caused by glioma].
abstract: OBJECTIVE
  To investigate the relationship between aquaporin-4 (AQP4) water channel expression in astrocytes and brain edema caused by glioma.
  
  
  METHODS
  The changes of water transport in in vitro blood-brain barrier models were examined using high-performance liquid chromatography (HPLC), and the expression level of AQP4 was assayed by semiquantitative RT-PCR.
  
  
  RESULTS
  The water transport of the in vitro blood-brain barrier model from the luminal side to basolateral side was increased after coculture with glioma cells, which induced significantly decreased expression level of AQP4 in the astrocytes.
  
  
  CONCLUSIONS
  Glioma cells can promote water transport in in vitro blood-brain barrier model from the luminal side to abluminal side, and the brain edema induced by the glioma cells may not necessarily be the result of hyperpermeability of the blood-brain barrier to macromolecules in the plasma. The decreased expression level of AQP4 induced by glioma cells may be a molecular mechanism for neoplastic brain edema in patients with glioma.
authors.affiliation: Department of Neurosurgery, First Military Medical University, Guangzhou 510282, China. chenyizhao@21cn.com
authors.forename: Yi-zhao
authors.initials: YZ
authors.lastname: Chen
authors.forename: Ru-xiang
authors.initials: RX
authors.lastname: Xu
authors.forename: Zong-jun
authors.initials: ZJ
authors.lastname: Xu
authors.forename: Zhi-lin
authors.initials: ZL
authors.lastname: Yang
authors.forename: Xiao-dan
authors.initials: XD
authors.lastname: Jiang
authors.forename: Ying-qian
authors.initials: YQ
authors.lastname: Cai
mesh_terms: D000818:Animals; D051401:Aquaporin 4; D020346:Aquaporins / Q000235:genetics*; D001253:Astrocytes / Q000378:metabolism*; D001812:Blood-Brain Barrier; D001834:Body Water / Q000378:metabolism; D001929:Brain Edema / Q000209:etiology*; D005910:Glioma / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C496032:Aqp4 protein, rat; D051401:Aquaporin 4; D020346:Aquaporins