Modeling of DNA microarray data by using physical properties of hybridization.
A method of analyzing DNA microarray data based on the physical modeling of hybridization is presented. We demonstrate, in experimental data, a correlation between observed hybridization intensity and calculated free energy of hybridization. Then, combining hybridization rate equations, calculated free energies of hybridization, and microarray data for known target concentrations, we construct an algorithm to compute transcript concentration levels from microarray data. We also develop a method for eliminating outlying data points identified by our algorithm. We test the efficacy of these methods by comparing our results with an existing statistical algorithm, as well as by performing a cross-validation test on our model.
Held
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GA
IBM Thomas J. Watson Research Center, Yorktown Heights, NY 10598, USA. gaheld@us.ibm.com
Grinstein
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G
Tu
Y
Y
D000465:Algorithms; D001703:Biophysics / Q000379:methods; D004305:Dose-Response Relationship, Drug; D006801:Humans; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D012984:Software; D013223:Statistics as Topic; D013816:Thermodynamics