[Multilocus sequence-typing for characterization of Moscow strains of Haemophilus influenzae type b].
Haemophilius influenzae, type b (Hib) bacteria, were genotyped by multilocus sequence typing (MLST) using 5 loci (adk, fucK, mdh, pgi, recA). 42 Moscow Hib strains (including 38 isolates form cerebrospinal fluid of children, who had purulent meningitis in 1999-2001, and 4 strains isolated from healthy carriers of Hib), as well as 2 strains from Yekaterinburg were studied. In MLST a strain is characterized, by alleles and their combinations (an allele profile) referred to also as sequence-type (ST). 9 Sts were identified within the Russian Hib bacteria: ST-1 was found in 25 strains (57%), ST-12 was found in 8 strains (18%), ST-11 was found in 4 strains (9%) and ST-15 was found in 2 strains (4.5%); all other STs strains (13, 14, 16, 17, 51) were found in isolated cases (2.3%). A comparison of allelic profiles and of nucleotide sequences showed that 93% of Russian isolates, i.e. strain with ST-1, 11, 12, 13, 15 and 17, belong to one and the same clonal complex. 2 isolates from Norway and Sweden from among 7 foreign Hib strains studied up to now can be described as belonging to the same clonal complex; 5 Hib strains were different from the Russian ones.
Platonov
A E
AE
Central Research Institute for Epidemiology, Ministry of Health of the Russian Federation, Russia, Moscow.
Mironov
K O
KO
Iatsyshina
S B
SB
Koroleva
I S
IS
Platonova
O V
OV
Gushchin
A E
AE
Shipulin
G A
GA
D000263:Adenylate Kinase / Q000235:genetics; D000483:Alleles; D002675:Child, Preschool; D029968:Escherichia coli Proteins; D005787:Gene Frequency; D005798:Genes, Bacterial; D005838:Genotype; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics; D019741:Haemophilus influenzae type b / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008291:Malate Dehydrogenase / Q000235:genetics; D008583:Meningitis, Haemophilus / Q000134:cerebrospinal fluid / Q000382:microbiology; D009031:Moscow; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics; D011938:Rec A Recombinases / Q000235:genetics; D017422:Sequence Analysis, DNA
D029968:Escherichia coli Proteins; D008291:Malate Dehydrogenase; D017853:Phosphotransferases (Alcohol Group Acceptor); C090544:L-fuculokinase, E coli; D000263:Adenylate Kinase; D011938:Rec A Recombinases; D005956:Glucose-6-Phosphate Isomerase