title: SPARC-null mice display abnormalities in the dermis characterized by decreased collagen fibril diameter and reduced tensile strength.
abstract: Although collagen and elastic fibers are among the major structural constituents responsible for the mechanical properties of skin, proteins that associate with these components are also important for directing formation and maintaining the stability of these fibers. We present evidence that SPARC (secreted protein acidic and rich in cysteine) contributes to collagen fibril formation in the dermis. The skin of SPARC-null adult mice had approximately half the tensile strength as that of wild-type skin. Moreover, the collagen content of SPARC-null skin, as measured by hydroxyproline analysis, was substantially reduced in adult mice. At 2 weeks of age, no differences in collagen content were observed; within 2 months, however, the dermis of SPARC-null mice displayed a reduced collagen content that persisted through adulthood until approximately 20 months, when collagen levels of SPARC-null skin approximated those of wild-type controls. The collagen fibrils present in SPARC-null skin were smaller and more uniform in diameter, in comparison with those of wild-type skin. At 5 months of age, the average fibril diameter in SPARC-null versus wild-type skin was 60.2 nm versus 87.9 nm, respectively. Extraction of soluble dermal collagen revealed a relative increase in collagen VI, accompanied by a decrease in collagen I, in SPARC-null mice. A reduction in the relative amounts of higher-molecular weight collagen complexes was also observed in extracts of dermis from SPARC-null animals. Thus the absence of SPARC compromises the mechanical properties of the dermis, an effect that we attribute, at least in part, to the changes in the structure and composition of its collagenous extracellular matrix.
authors.affiliation: The Hope Heart Institute, Seattle, Washington 98104, USA.
authors.forename: Amy D
authors.initials: AD
authors.lastname: Bradshaw
authors.forename: Pauli
authors.initials: P
authors.lastname: Puolakkainen
authors.forename: Jayasri
authors.initials: J
authors.lastname: Dasgupta
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Davidson
authors.forename: Thomas N
authors.initials: TN
authors.lastname: Wight
authors.forename: E
authors.initials: E
authors.lastname: Helene Sage
mesh_terms: D019342:Acetic Acid; D000818:Animals; D003094:Collagen / Q000737:chemistry / Q000648:ultrastructure*; D020405:Dermis / Q000002:abnormalities* / Q000473:pathology / Q000503:physiopathology* / Q000648:ultrastructure; D006909:Hydroxyproline / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D008970:Molecular Weight; D015676:Osteonectin / Q000172:deficiency* / Q000378:metabolism; D020033:Protein Isoforms / Q000737:chemistry; D012995:Solubility; D013718:Tensile Strength
chemical_list: D015676:Osteonectin; D020033:Protein Isoforms; D003094:Collagen; D019342:Acetic Acid; D006909:Hydroxyproline