title: Tc-99m-HMPAO uptake by bronchoalveolar cells.
abstract: Lung uptake of intravenously injected Tc-99m-HMPAO is observed in smokers and in lung toxicity due to various agents. We investigated the Tc-99m-HMPAO uptake of bronchoalveolar lavage (BAL) cells in the lungs after incubation in in vitro conditions (6 patients), intravenous injection (IV) (7 patients) and inhalation (INH) (6 patients) of Tc-99m-HMPAO in order to show whether BAL cells are also responsible for Tc-99m-HMPAO uptake in the lungs. Cell/supernatant (C/S) count ratio was 7.0 +/- 3.5, 29.3 +/- 40.8 and 8.4 +/- 4.5 for in vitro, IV and INH groups, respectively. C/Sin vitro showed a positive correlation with % alveolar macrophages (r = 0.943, p = 0.0048) and a negative correlation with % neutrophils (r = -0.945, p = 0.0045). Cells/whole BAL fluid ratio correlated with the amount of daily cigarette consumption in INH group (r = 0.95, p = 0.0037). Tc-99m-HMPAO showed adherence to mucus after inhalation. Tc-99m-HMPAO diffuses into alveolar spaces after injection and is present in BAL fluid and BAL cells both after injection and inhalation. Glutathione concentration and oxido-reductive state of the epithelial lining fluid and BAL cells may influence the lung uptake of Tc-99m-HMPAO.
authors.affiliation: Department of Nuclear Medicine, Dokuz Eylül University School of Medicine, Inciralti, Izmir, Turkey. hatice.durak@deu.edu.tr
authors.forename: Hatice
authors.initials: H
authors.lastname: Durak
authors.forename: Oğuz
authors.initials: O
authors.lastname: Kilinç
authors.forename: Türkan
authors.initials: T
authors.lastname: Ertay
authors.forename: Eyüp Sabri
authors.initials: ES
authors.lastname: Uçan
authors.forename: Aydanur
authors.initials: A
authors.lastname: Kargi
authors.forename: Gamze Capa
authors.initials: GC
authors.lastname: Kaya
authors.forename: Banu
authors.initials: B
authors.lastname: Sis
mesh_terms: D000280:Administration, Inhalation; D000336:Aerosols; D001980:Bronchi / Q000000981:diagnostic imaging / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry* / Q000166:cytology*; D005260:Female; D006801:Humans; D007275:Injections, Intravenous; D016676:Macrophages, Alveolar / Q000000981:diagnostic imaging / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011650:Pulmonary Alveoli / Q000000981:diagnostic imaging / Q000378:metabolism; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000008:administration & dosage / Q000493:pharmacokinetics*; D012907:Smoking / Q000378:metabolism*; D013223:Statistics as Topic; D019690:Technetium Tc 99m Exametazime / Q000008:administration & dosage* / Q000493:pharmacokinetics*
chemical_list: D000336:Aerosols; D019275:Radiopharmaceuticals; D019690:Technetium Tc 99m Exametazime