[Effects of plasmid pKM101 on the expression of Escherichia coli and Salmonella typhimurium genes under ultraviolet irradiation].
The study focused on plasmid pKM101, which is a necessary component of the short-term test of Eim's system (Salmonella-microsome test), to detect the potential carcinogens through their mutagen activity. We found a previously unknown feature of the plasmid to enhance the expression of certain plasmid and chromosome genes. The purpose of the present study was to examine and specify the role of operon mucAB responsible for the mutation properties of the plasmid in activating the expression of bacterial genes. An ultraviolet-induction examination of bacterial genes, with the mutants of plasmid pKM101 affecting operon mucAB being used, showed that the function of genes mucAB did activate, but, on the contrary, suppressed the induction of genes elt (i.e. of genes controlling the formation of LT-toxin of Escherichia coli) and of sfiA (SOS-regulated gen E. col controlling the cell division.
Tiganova
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IG
Gamaleya's Research Institute for Epidemiology and Microbiology, Russian Academy of Medical Sciences, Moscow, Russia.
Rusina
O Iu
OIu
Andreeva
I V
IV
Skavronskaia
A G
AG
D001427:Bacterial Toxins / Q000235:genetics; D002455:Cell Division; D004768:Enterotoxins / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000528:radiation effects*; D029968:Escherichia coli Proteins / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000528:radiation effects; D005798:Genes, Bacterial / Q000528:radiation effects*; D009154:Mutation; D009876:Operon; D010957:Plasmids / Q000235:genetics*; D012486:Salmonella typhimurium / Q000235:genetics* / Q000528:radiation effects; D013489:Suppression, Genetic; D014466:Ultraviolet Rays
D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C053139:sulA protein, E coli; C068606:mucAB protein, E coli; C027538:heat-labile enterotoxin, E coli