The use of immobilized methylchymotrypsin for the purification of human and sheep alpha-1-proteinase inhibitor (alpha(1)-PI).
alpha(1)-proteinase inhibitor was isolated from albumin fractions of human and sheep plasma using a new method of purification using affinity chromatography on immobilized methylchymotrypsin in the presence of 5 M NaCl. The inhibitor was finally polished to homogenity either by chromatography on a Mono Q or a Sephacryl S-200 HR column. The presented method makes it possible to recover alpha(1)-proteinase inhibitor which has been added to cow milk.
Grybel
Joanna
J
Institute of Biochemistry and Molecular Biology, University of Wrocław, Tamka 2, 50-137 Wrocław, Poland. joanna@bf.uni.wroc.pl
Wilusz
Tadeusz
T
D000818:Animals; D002918:Chymotrypsin / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006801:Humans; D008892:Milk / Q000378:metabolism; D012756:Sheep; D000515:alpha 1-Antitrypsin / Q000302:isolation & purification* / Q000378:metabolism
D004800:Enzymes, Immobilized; D000515:alpha 1-Antitrypsin; D002918:Chymotrypsin