Measurement of acetylcholine-induced endothelium-derived nitric oxide in aorta using a newly developed catheter-type nitric oxide sensor.
Intra-aortic measurement of nitric oxide (NO) would provide valuable insights into NO bioavailability in systemic circulation and vascular endothelial function. In the present study, we thus developed a catheter-type NO sensor to measure intra-aortic NO concentration in vivo. An NO sensor was encased and fixed in a 4-Fr catheter. The sensor was then located in the thoracic aorta via the femoral artery through a 7-Fr catheter to measure intra-aortic plasma NO concentration in vivo in anesthetized dogs. Infusion of acetylcholine (10 microg/kg) increased base-to-peak plasma NO level in the aorta by 2.4+/-0.4 nM (n=7). After 20-min infusion of N(G)-methyl-L-arginine (NO synthase inhibitor), changes in plasma NO concentration in response to acetylcholine were attenuated significantly (1.8+/-0.4 nM, P<0.003, n=7). In conclusion, the newly developed catheter-type NO sensor successfully measured acetylcholine-induced changes in intra-aortic plasma concentration of endothelium-derived NO in vivo and demonstrated applicability to direct evaluation of intravascular NO bioavailability.
Mochizuki
Seiichi
S
Department of Medical Engineering, Kawasaki Medical School, 577 Matsushima, Kurashiki, 701-0192, Okayama, Japan. mochi@me.kawasaki-m.ac.jp
Miyasaka
Takehiro
T
Goto
Masami
M
Ogasawara
Yasuo
Y
Yada
Toyotaka
T
Akiyama
Maki
M
Neishi
Yoji
Y
Toyoda
Tomohiko
T
Tomita
Junko
J
Koyama
Yuji
Y
Tsujioka
Katsuhiko
K
Kajiya
Fumihiko
F
Akasaka
Takashi
T
Yoshida
Kiyoshi
K
D000109:Acetylcholine / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D001011:Aorta / Q000378:metabolism*; D002404:Catheterization / Q000295:instrumentation; D004285:Dogs; D004566:Electrodes; D004727:Endothelium / Q000166:cytology / Q000378:metabolism; D005260:Female; D008297:Male; D009569:Nitric Oxide / Q000032:analysis* / Q000097:blood; D013997:Time Factors
D009569:Nitric Oxide; D000109:Acetylcholine