[Expression of regulatory genes Oct-4, Pax-6, Prox-1, Ptx-2 at the initial stages of differentiation of embryonic stem cells in vitro].
The expression of regulatory genes of the POU, Pax, Prox, and Ptx gene families was studied at the initial stages of differentiation of murine embryonic stem cells of R1 line. mRNAs were isolated from undifferentiated embryonic stem cells and embryoid bodies formed at the early stages of in vitro differentiation and cDNA sequences were synthesized for comparative PCR analysis of the expression of studied genes. The levels of expression of the gene Oct-4 involved in maintenance of the pluripotent status of embryonic stem cells proved to be practically indistinguishable in undifferentiated cells and embryoid bodies, while the expression of Pax-6 markedly increased in the latter. The levels and patterns of expression of the homeobox transcription factors Prox-1 and Ptx-2 were compared on this cell model for the first time. A probable role of these genes in differentiation of the murine embryonic stem cells is discussed.
Gordeeva
O F
OF
Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, ul. Vavilova 26, Moscow, 119991 Russia. olgagordeeva@yandex.ru
Manuilova
E S
ES
Grivennikov
I A
IA
Smirnova
Iu A
IuA
Krasnikova
N Iu
NIu
Zinov'eva
R D
RD
Khrushchov
N G
NG
D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000235:genetics*; D004622:Embryo, Mammalian / Q000166:cytology; D005136:Eye Proteins; D018507:Gene Expression Regulation, Developmental; D005809:Genes, Regulator; D018398:Homeodomain Proteins / Q000235:genetics*; D051379:Mice; D008815:Mice, Inbred Strains; D009687:Nuclear Proteins; D050814:Octamer Transcription Factor-3; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000502:physiology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; D000097577:Homeobox Protein PITX2; D000098551:Prospero-Related Homeobox 1 Protein
D004268:DNA-Binding Proteins; D005136:Eye Proteins; D018398:Homeodomain Proteins; D009687:Nuclear Proteins; D050814:Octamer Transcription Factor-3; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; D012333:RNA, Messenger; D012097:Repressor Proteins; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; D000097577:Homeobox Protein PITX2; D000098551:Prospero-Related Homeobox 1 Protein; C000605732:Pax6 protein, mouse; C494174:Pou5f1 protein, mouse