Towards a standardized format for the description of a novel species (of an established genus): Ochrobactrum gallinifaecis sp. nov.
A format for the description of single novel species is proposed, which should facilitate the reviewing process by assisting the provision of data in a standardized form. The abstract must be short and concise, highlighting phylogenetic position, morphology and chemotaxonomy for genus affiliation, the genotypic and phenotypic basis for species differentiation, and the name and deposition numbers from two public culture collections in different countries for the type strain: A Gram-negative, rod-shaped, non-spore-forming bacterium (Iso 196(T)) was isolated from chicken faeces. On the basis of 16S rRNA gene sequence similarity, strain Iso 196(T) was shown to belong to the alpha-2 subclass of the Proteobacteria related to Ochrobactrum tritici (95.6%), Ochrobactrum grignonense (95.0%) and Ochrobactrum anthropi (94.6%), and the phylogenetic distance from any validly described species within the genus Brucella was less than 95%. Chemotaxonomic data (major ubiquinone - Q-10; major polyamines - spermidine and putrescine; major polar lipids - phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine; major fatty acids - C(18 : 1)omega7c and C(19 : 0) cyclo omega8c) supported the affiliation of strain Iso 196(T) to the genus Ochrobactrum. The results of DNA-DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain Iso 196(T) from the four validly published Ochrobactrum species. Iso 196(T) therefore represents a new species, for which the name Ochrobactrum gallinifaecis sp. nov. is proposed, with the type strain Iso 196(T) (= DSM 15295(T) = CIP 107753(T)).
Kämpfer
Peter
P
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
Buczolits
Sandra
S
Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Wien, Austria.
Albrecht
Andreas
A
Bundesanstalt für Arbeitsschutz und Arbeitsmedizin, D-10317 Berlin, Germany.|Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
Busse
Hans-Jürgen
HJ
Institut für Mikrobiologie und Genetik, Universität Wien, A-1030 Wien, Austria.|Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Wien, Austria.
Stackebrandt
Erko
E
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen, Mascheroder Weg 1b, D-38124 Braunschweig, Germany.
D015373:Bacterial Typing Techniques / Q000592:standards*; D002002:Brucella / Q000145:classification* / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D002965:Classification / Q000379:methods*; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D005838:Genotype; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S