title: Non-responders to egg grown influenza vaccine seroconvert after booster immunization with MDCK cell grown vaccine.
abstract: We have investigated whether 'at risk' subjects who did not respond serologically during a pre-study vaccination with a commercial egg grown influenza sub-unit vaccine would respond to a subsequent vaccination with either a single dose of MDCK cell grown influenza vaccine or a standard egg grown influenza vaccine containing the same virus strains. We studied 48 non-responder subjects with a mean age 67.5, range: 34-82 years. In this non-responder group the increased immune response that was detected after boosting with an MDCK cell derived vaccine response was variable and relatively modest, except for the A/Texas strain in the vaccine. The proportion of subjects, with an HI titre of >/=40 (protective antibody titre) increased from 50 to 83% (A/Texas strain), from 13 to 25% (B/Harbin strain) and from 38 to 46% (A/Wuhan strain). In comparison a booster vaccination with egg-derived influenza vaccine resulted in an increase immune response with an HI antibody titre >/=40 for two of the three strains, namely from 17 to 58% for the B/Harbin strain and from 8 to 33% for the A/Wuhan strain.
authors.affiliation: Department of Medical Microbiology and Retroscreen Virology, Queen Mary's School of Medicine and Dentistry, St Bart's and the London, 327 Mile End Road, London E1 4NS, UK.
authors.forename: John S
authors.initials: JS
authors.lastname: Oxford
authors.forename: Ali A
authors.initials: AA
authors.lastname: Al-Jabri
authors.forename: Robert
authors.initials: R
authors.lastname: Lambkin
authors.forename: A M
authors.initials: AM
authors.lastname: Palache
authors.forename: D M
authors.initials: DM
authors.lastname: Fleming
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D002460:Cell Line; D002642:Chick Embryo; D004285:Dogs; D005260:Female; D006801:Humans; D007117:Immunization, Secondary; D009980:Influenza A virus / Q000276:immunology; D009981:Influenza B virus / Q000276:immunology; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000009:adverse effects / Q000276:immunology*; D007273:Injections, Intramuscular; D008297:Male; D008875:Middle Aged; D011795:Surveys and Questionnaires; D014611:Vaccination
chemical_list: D000914:Antibodies, Viral; D007252:Influenza Vaccines