title: Characterization of Hodgkin's lymphoma-like undifferentiated carcinoma of the nasopharyngeal type as a particular UCNT subtype mimicking Hodgkin's lymphoma.
abstract: Undifferentiated carcinoma of the nasopharyngeal type (UCNT) that histologically mimics Hodgkin's lymphoma (HL) ("Hodgkin's lymphoma-like UCNT"--HL-like UCNT) is known as a diagnostic pitfall. Using immunohistochemistry, Western blot and cDNA array technology, we wanted to document its phenotypical and molecular characteristics. We report herein 5 cases of UCNT that morphologically mimic HL and 3 classical UCNT cases. We compared the expression profiles of a thousand selected genes in HL-like UCNT and in classical UCNT cases. No difference in the profile of EBV infection was noted between the HL-like UCNT and control cases. Significant differences were detected in the expression of genes involved in the matrix modelling, angiogenesis, apoptosis and regulation of the Th-2 interleukins. The eosinophil chemoattractant eotaxin was expressed in the stroma of HL-like UCNT, but not in the control cases. The eotaxin receptor CCR3 was expressed in both stromal and carcinoma cell populations of HL-like UCNT, this pattern being similar to the one observed in HL. These results show that UCNT morphologically resembling HL share also some specific phenotypical and molecular features with HL, and might deserve to be isolated as a particular UCNT subtype.
authors.affiliation: Department of Pathology, Institut Paoli-Calmettes, 232 Boulevard de Sainte-Marguerite, BP 156, F-13273 Marseille cedex 9, France. jauffrete@marseille.fnclcc.fr
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Charafe-Jauffret
authors.forename: François
authors.initials: F
authors.lastname: Bertucci
authors.forename: Olivier
authors.initials: O
authors.lastname: Ramuz
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Devilard
authors.forename: Philippe
authors.initials: P
authors.lastname: Gaulard
authors.forename: Pierre
authors.initials: P
authors.lastname: Brousset
authors.forename: Remi
authors.initials: R
authors.lastname: Houlgatte
authors.forename: J
authors.initials: J
authors.lastname: Hassoun
authors.forename: Daniel
authors.initials: D
authors.lastname: Birnbaum
authors.forename: Luc
authors.initials: L
authors.lastname: Xerri
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D002277:Carcinoma / Q000145:classification / Q000473:pathology*; D018076:DNA, Complementary / Q000378:metabolism; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D004854:Herpesvirus 4, Human / Q000235:genetics; D006689:Hodgkin Disease / Q000145:classification / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D017403:In Situ Hybridization; D007438:Introns; D008223:Lymphoma / Q000145:classification / Q000473:pathology*; D009303:Nasopharyngeal Neoplasms / Q000145:classification / Q000473:pathology*; D009389:Neovascularization, Pathologic; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype
chemical_list: D018076:DNA, Complementary