title: Trypanosoma cruzi: susceptibility to chemotherapy with benznidazole of clones isolated from the highly resistant Colombian strain.
abstract: The present investigation was performed to evaluate the susceptibility of seven clones isolated from the highly resistant Colombian strains, prototype of Biodeme Type III. Seven clones previously obtained, showed a phenotypic homogeneity and high similarity with the parental strain. Eight groups of 30 mice were inoculated with one of seven clones or the parental strain; 20 were treated with benznidazole (100mg/kg/day) and 10 were untreated controls. Cure evaluations were done by parasitological and serological tests and PCR. Cure rates varied from 0% (null) to 16.7%. Correlation between positivity of parasitological and serological tests with positive PCR reached 37%. The results demonstrated the high resistance of the clones, suggesting the predominance of a highly resistant principal clone in this strain. The findings apparently indicate that the possibility of cure is minimal for patients infected with this biodeme; a fact that could affect the control of Chagas' disease through treatment of chronically infected people.
authors.affiliation: Laboratorio de Doença de Chagas Experimental, Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, BA, Brasil.
authors.forename: Edson L P
authors.initials: EL
authors.lastname: Camandaroba
authors.forename: Eliana A G
authors.initials: EA
authors.lastname: Reis
authors.forename: Marilda S
authors.initials: MS
authors.lastname: Gonçalves
authors.forename: Mitermayer G
authors.initials: MG
authors.lastname: Reis
authors.forename: Sonia G
authors.initials: SG
authors.lastname: Andrade
mesh_terms: D000818:Animals; D014355:Chagas Disease / Q000188:drug therapy* / Q000469:parasitology; D003001:Cloning, Molecular; D003105:Colombia; D004351:Drug Resistance; D051379:Mice; D009593:Nitroimidazoles / Q000494:pharmacology* / Q000627:therapeutic use; D018512:Parasitemia / Q000188:drug therapy; D010274:Parasitology / Q000379:methods; D016133:Polymerase Chain Reaction; D014344:Trypanocidal Agents / Q000494:pharmacology* / Q000627:therapeutic use; D014349:Trypanosoma cruzi / Q000187:drug effects*
chemical_list: D009593:Nitroimidazoles; D014344:Trypanocidal Agents; C009999:benzonidazole