title: Identification of genes showing differential expression in anorexia mutant mouse.
abstract: Differential display (DD) PCR was applied to identify genes regulated by anorexia (anx) in mouse brain. Of the four apparently differentially expressed genes, three (H2-H4) had high homology with known genes such as apoptotic protease activating factor 1 (Apaf 1), adenylate cyclase 6 (Adcy 6), and myelin proteolipid protein (PLP). H1, designated as HIPA1, had a homology with unknown function gene fragments. Northern blot analysis confirmed HIPA1 expression induced by anorexia. A 1579 bp full-length cDNA of HIPA1 was isolated from a mouse brain cDNA library using a probe from the differentially displayed H1 fragment. Sequence analysis showed that HIPA1 had 98.7% homology to mouse hippocampus cDNA. In situ hybridization demonstrated that the HIPA1 mRNA was highly up-regulated in the hippocampus of anx/anx mouse brain.
authors.affiliation: Division of Biological Science, Chosun University, Gwangju, South Korea. hsjeon@chosun.ac.kr
authors.forename: Hong Sung
authors.initials: HS
authors.lastname: Chun
authors.forename: Yeol
authors.initials: Y
authors.lastname: Park
authors.forename: Young Ki
authors.initials: YK
authors.lastname: Yang
authors.forename: Do Kyung
authors.initials: DK
authors.lastname: Kim
authors.forename: Jin H
authors.initials: JH
authors.lastname: Son
authors.forename: Sung-Jun
authors.initials: SJ
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D000818:Animals; D000855:Anorexia / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence / Q000235:genetics; D005786:Gene Expression Regulation / Q000502:physiology*; D006624:Hippocampus / Q000378:metabolism; D051379:Mice; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D012333:RNA, Messenger