Detection and characterization of the Gloeosporium gloeosporioides growth inhibitory compound iturin A from Bacillus subtilis strain KS03.
The Bacillus subtilis strain KS03 was isolated, and identified as a biological control agent that inhibits the anthracnose disease fungus Gloeosporium gloeosporioides. The antifungal compound was purified from its culture broth through butanol extraction, diethylaminoethyl (DEAE) Sepharose CL-6B chromatography, and preparative thin layer chromatography. Tandem mass spectrometric analyses (MS/MS), with matrix-assisted laser desorption ionization (MALDI) time-of-fight/time-of-flight (TOF/TOF) mass spectrometry, showed that the antifungal compound was iturin A, a cyclic lipopeptide antibiotic. The major compound, with a molecular mass of 1042 Da, was identified as iturin A(2).
Cho
Soo-Jin
SJ
Department of Microbiology, College of Sciences, Daejeon University, 300-716, Daejeon, South Korea.
Lee
Sam Keun
SK
Cha
Byeong Jin
BJ
Kim
Young Hwan
YH
Shin
Kwang-Soo
KS
D000900:Anti-Bacterial Agents / Q000032:analysis / Q000494:pharmacology*; D000935:Antifungal Agents / Q000032:analysis / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000737:chemistry* / Q000235:genetics; D003904:Mitosporic Fungi / Q000187:drug effects* / Q000254:growth & development; D010455:Peptides; D010456:Peptides, Cyclic; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D010455:Peptides; D010456:Peptides, Cyclic; D012336:RNA, Ribosomal, 16S; C013579:iturin A