title: Simultaneous analysis of seventeen chemical ingredients of Ligusticum chuanxiong by on-line high performance liquid chromatography-diode array detector-mass spectrometry.
abstract: An on-line high performance liquid chromatography (HPLC)-diode array detector (DAD)-mass spectrometry (MS) analytical method has been developed to simultaneously separate and identify seventeen main constituents of Chuanxiong ( Ligusticum chuanxiong Hort.), a traditional Chinese medicinal herb. In three Chuanxiong samples, nine compounds were unequivocally determined as vanillin (1), ferulic acid (2), senkyunolide I (4), senkyunolide H (5), senkyunolide A (6), coniferyl ferulate (7), Z-ligustilide (8), neocnidilide (9) and 3-butylidenephthalide (10) by comparing their t R, UV, and MS data with those obtained for the authentic compounds. Furthermore, additional eight compounds were tentatively identified as senkyunolide J (11), senkyunolide F (12), 3-butylphthalide (13), cnidilide (14), riligustilide (15) or Z,Z'-6,8',7,3'-diligustilide (16), tokinolide B (17), levistolide A (18) and senkyunolide P (19), based on their MS data and the comparison of their UV spectra with the published references. This is the first report to describe the development of an on-line HPLC-DAD-MS method to simultaneously analyse seventeen main constituents present in Chuanxiong and to construct the chemical profiles of various Chuanxiong samples.
authors.affiliation: Department of Pharmacology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, N. T., Hong Kong SAR.
authors.forename: Song-Lin
authors.initials: SL
authors.lastname: Li
authors.forename: Sunny Sun-Kin
authors.initials: SS
authors.lastname: Chan
authors.forename: Ge
authors.initials: G
authors.lastname: Lin
authors.forename: Lei
authors.initials: L
authors.lastname: Ling
authors.forename: Ru
authors.initials: R
authors.lastname: Yan
authors.forename: Hoi-Sing
authors.initials: HS
authors.lastname: Chung
authors.forename: Yun-Kau
authors.initials: YK
authors.lastname: Tam
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal; D006801:Humans; D030004:Ligusticum; D013058:Mass Spectrometry; D008516:Medicine, Chinese Traditional; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; C101574:chuangxiong extract