Are UV-induced nonculturable Escherichia coli K-12 cells alive or dead?
Cells that have lost the ability to grow in culture could be defined operationally as either alive or dead depending on the method used to determine cell viability. As a consequence, the interpretation of the state of 'nonculturable' cells is often ambiguous. Escherichia coli K12 cells inactivated by UV-irradiation with a low (UV1) and a high (UV2) dose were used as a model of nonculturable cells. Cells inactivated by the UV1 dose lost 'culturability' but they were not lysed and maintained the capacity to respond to nutrient addition by protein synthesis and cell wall synthesis. The cells also retained both a high level of glucose transport and the capacity for metabolizing glucose. Moreover, during glucose incorporation, UV1-treated cells showed the capacity to respond to aeration conditions modifying their metabolic flux through the Embden-Meyerhof and pentose-phosphate pathways. However, nonculturable cells obtained by irradiation with the high UV2 dose showed several levels of metabolic imbalance and retained only residual metabolic activities. Nonculturable cells obtained by irradiation with UV1 and UV2 doses were diagnosed as active and inactive (dying) cells, respectively.
Villarino
Andrea
A
Aquabiolab and Unité de Biodiversité des Bactéries Pathogènes Emergentes, INSERM U389, Institut Pasteur, Paris, France.
Rager
Marie-Noëlle
MN
Grimont
Patrick A D
PA
Bouvet
Odile M M
OM
D000332:Aerobiosis; D001431:Bacteriological Techniques / Q000379:methods; D004307:Dose-Response Relationship, Radiation; D004926:Escherichia coli / Q000166:cytology / Q000254:growth & development* / Q000528:radiation effects*; D029968:Escherichia coli Proteins / Q000096:biosynthesis; D005434:Flow Cytometry; D005947:Glucose / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008715:Methionine / Q000378:metabolism; D008856:Microscopy, Fluorescence; D010427:Pentose Phosphate Pathway; D014466:Ultraviolet Rays
D029968:Escherichia coli Proteins; D008715:Methionine; D005947:Glucose