title: Glutaraldehyde-fixed kangaroo aortic wall tissue: histology, crosslink stability and calcification potential.
abstract: Stentless aortic heart valve substitutes, manufactured from biological tissues, are fixed with glutaraldehyde to cross-link collagen, reduce antigenicity, and sterilize the tissue. Despite improved cross linking, reduced antigenicity, and various anticalcification measures, the aortic wall tissue present in these prostheses tends to calcify. The aim of this study was to assess the morphology, collagen cross-link stability, and calcification potential of glutaraldehyde-preserved kangaroo aortic wall tissue as opposed to porcine aortic wall tissue. Porcine and kangaroo aortic wall tissues were fixed in 0.625% buffered glutaraldehyde. Histology and cross-link stability were examined. Calcification potential was determined in the subcutaneous rat model. Kangaroo aortic wall tissue was significantly (p < 0.01) less calcified than porcine aortic wall tissue (26.67 +/- 6.53 versus 41.959 +/- 2.75 microg/mg tissue) at 8 weeks. In conclusion, the histological differences between kangaroo and porcine aortic wall tissue correlate well with the reduced calcification potential of kangaroo aortic wall tissue. The reduced calcification potential could result in improved long-term durability of stentless kangaroo heart valves as bioprostheses.
authors.affiliation: Fremantle Heart Institute & University of Western Australia, Fremantle Hospital, Fremantle, Western Australia. Leon.Neething@health.wa.gov.au
authors.forename: W M L
authors.initials: WM
authors.lastname: Neethling
authors.forename: A J
authors.initials: AJ
authors.lastname: Hodge
authors.forename: R
authors.initials: R
authors.lastname: Glancy
mesh_terms: D000818:Animals; D001705:Bioprosthesis / Q000009:adverse effects; D002114:Calcinosis / Q000209:etiology; D002118:Calcium / Q000032:analysis; D003094:Collagen / Q000032:analysis; D003432:Cross-Linking Reagents; D004549:Elastin / Q000032:analysis; D005404:Fixatives; D005976:Glutaral; D006350:Heart Valve Prosthesis / Q000009:adverse effects; D007614:Macropodidae; D008297:Male; D008422:Materials Testing; D051381:Rats; D017208:Rats, Wistar; D034421:Sus scrofa; D016707:Tissue Fixation
chemical_list: D003432:Cross-Linking Reagents; D005404:Fixatives; D003094:Collagen; D004549:Elastin; D002118:Calcium; D005976:Glutaral