title: Selective enhancement of B cell antigen receptor-mediated antigen presentation by treatment with transforming growth factor-beta.
abstract: TGF-beta1 was examined for the ability to regulate Ag-presentation by B cells, using A20-HL B lymphoma cells bearing TNP-specific IgM receptors. Treatment of A20-HL cells with TGF-beta1 at 1 ng/ml, a concentration that inhibited proliferation, enhanced presentation of Ag internalized via surface IgM (sIgM), but not via fluid-phase pinocytosis. TGF-beta1-treatment slightly enhanced surface expression of sIgM, but not of MHC class II molecules. The treatment accelerated recovery of sIgM expression after its removal by ligation with TNP-OVA, and induced prolonged intracellular residence of TNP-OVA internalized via sIgM, which co-localized with intracellular MHC class II molecules. TGF-beta1-treatment increased accumulation of newly synthesized intracellular MHC class II molecules that were localized in compartments positive for lysosome-associated membrane protein 1, although cellular protein synthesis was decreased by the treatment. The accumulated intracellular MHC class II molecules were triggered to the cell surface by ligation of sIgM. Finally, TGF-beta1-treatment induced Igalpha-phosphorylation in response to lower concentrations of TNP-OVA. On the basis of these findings, we conclude that TGF-beta1-treatment of A20-HL cells selectively enhances the ability to present Ag internalized via sIgM, not via fluid-phase pinocytosis, through accelerating sIgM recovery, increasing accumulation of intracellular MHC class II molecules and enhancing the ability of sIgM ligation to induce Igalpha-phosphorylation.
authors.affiliation: Department of Immunology, Toho University School of Medicine, 5-21-16 Omorinishi, Ota-ku, Tokyo 143-8540, Japan.
authors.forename: Chiaki
authors.initials: C
authors.lastname: Arai
authors.forename: Takamasa
authors.initials: T
authors.lastname: Ichijo
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Okada
authors.forename: Masanori
authors.initials: M
authors.lastname: Umeda
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Uchida
authors.forename: Mamoru
authors.initials: M
authors.lastname: Kiniwa
authors.forename: Terutaka
authors.initials: T
authors.lastname: Kakiuchi
mesh_terms: D017951:Antigen Presentation / Q000187:drug effects / Q000276:immunology*; D006801:Humans; D011947:Receptors, Antigen, B-Cell / Q000187:drug effects / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
chemical_list: D011947:Receptors, Antigen, B-Cell; D016212:Transforming Growth Factor beta