Genetic diversity of Gallibacterium anatis isolates from different chicken flocks.
Amplified fragment length polymorphisms (AFLPs) were used to characterize the genotypic diversity of a total of 114 Gallibacterium anatis isolates originating from a reference collection representing 15 biovars from four countries and isolates obtained from tracheal and cloacal swab samples of chickens from an organic, egg-producing flock and a layer parent flock. A subset of strains was also characterized by pulsed-field gel electrophoresis and biotyping. The organic flock isolates were characterized by more than 94% genetic similarity, indicating that only a single clone was apparent in the flock. The layer parent flock isolates were grouped into two subclusters, each with similarity above 90%. One subcluster contained only tracheal isolates, while the other primarily included cloacal isolates. In conclusion, we show that AFLP analysis enables fingerprinting of G. anatis, which seems to have a clonal population structure within natural populations. There was further evidence of clonal lineages, which may have adapted to different sites within the same animal.
Bojesen
Anders Miki
AM
Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, DK-1870 Frederiksberg C. The Danish Veterinary Institute, DK-1790 Copenhagen V, Denmark. miki@kvl.dk
Torpdahl
Mia
M
Christensen
Henrik
H
Olsen
John Elmerdahl
JE
Bisgaard
Magne
M
D000818:Animals; D015373:Bacterial Typing Techniques; D002645:Chickens / Q000145:classification / Q000382:microbiology*; D002988:Cloaca / Q000382:microbiology; D014644:Genetic Variation; D016905:Gram-Negative Bacterial Infections / Q000453:epidemiology / Q000382:microbiology; D015661:Pasteurellaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012150:Polymorphism, Restriction Fragment Length; D011201:Poultry Diseases / Q000382:microbiology; D014132:Trachea / Q000382:microbiology