Identification of lactic acid bacteria isolated from human vaginal secretions.
A total of 57 lactic acid bacteria were isolated from the vaginal secretions of 259 patients. Of these strains, 37 were isolated from patients attending pre-natal clinics and the remaining strains from patients attending post-natal clinics. The strains were identified by using simple physiological and biochemical tests and their phenotypic relatedness determined by numerical analysis of total soluble cell protein patterns. The genotypic relatedness of representative strains selected from each of the protein profile clusters was determined by numerical analysis of the DNA banding patterns obtained from RAPD-PCR. The majority of lactobacilli isolated belonged to the species Lactobacillus pentosus, Lactobacillus fermentum and Enterococcus faecium. A few strains of Lactobacillus plantarum and Weissella viridescens were also isolated. One strain, TV 1029, grouped into the same protein profile cluster as E. faecium, but revealed a DNA banding pattern closer related to Enterococcus faecalis. This is the first report of W. viridescens associated with the human vagina.
Silvester
M E
ME
Department of Microbiology, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa.
Dicks
L M T
LM
D001426:Bacterial Proteins / Q000737:chemistry; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D016983:Enterococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism*; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D014621:Vagina / Q000382:microbiology*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D019344:Lactic Acid