Production of superoxide dismutase by Deinococcus radiophilus.
The production of superoxide dismutase (SOD) varied in Deinococcus radiophilus, the UV resistant bacterium, depending upon different phases of growth, UV irradiation, and superoxide treatment. A gradual increase in total SOD activity occurred up to the stationary phases. The electrophoretic resolution of the SOD in cell extracts of D. radiophilus at each growth phase revealed the occurrence of MnSOD throughout the growth phases. The SOD profiles of D. radiophilus at the exponential phase received oxidative stress by the potassium superoxide treatment or UV irradiation also revealed the occurrence of a single SOD. However, these treatments caused an increase in SOD activity. The data strongly suggest that D. radiophilus has only one species of SOD as a constitutive enzyme, which seems to be a membrane-associated protein.
Yun
Young Sun
YS
Division of Life Sciences, Chungbuk National University, Cheongju, Chungbuk 361-763, Korea.
Lee
Young Nam
YN
D002462:Cell Membrane / Q000201:enzymology; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D006095:Gram-Positive Cocci / Q000187:drug effects / Q000201:enzymology* / Q000528:radiation effects; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000096:biosynthesis* / Q000378:metabolism; D013481:Superoxides / Q000494:pharmacology; D013997:Time Factors; D014466:Ultraviolet Rays
D013481:Superoxides; C039316:potassium superoxide; D013482:Superoxide Dismutase