title: Effect of vitamin E and eccentric exercise on selected biomarkers of oxidative stress in young and elderly men.
abstract: Muscle damage resulting from eccentric exercise provides a useful model of oxyradical-induced injury and can be used to examine age-related responses to oxidative stress. Sixteen young (26.4 +/- 3.3 years) and 16 older (71.1 +/- 4.0 years) healthy men were randomly assigned to 1000 IU/d vitamin E or placebo for 12 weeks and ran downhill for 45 min at 75% VO(2)max, once before and following supplementation. Blood samples were obtained before (baseline) and immediately postexercise (0 h), and at 6, 24, and 72 h postexercise to determine antioxidant status, muscle damage, lipid peroxidation, and DNA damage. Following exercise, young and older men experienced similar increases in serum creatine kinase (CK), F(2alpha)-isoprostanes (iPF(2alpha); p <.001) and malondialdehyde (MDA; p <.01), although iPF(2alpha) peaked at 72 h postexercise and MDA peaked at 0 h. Oxygen Radical Absorbance Capacity (ORAC) decreased at 72 h (p <.01) and correlated with the rise in iPF(2alpha), MDA, and CK in the young men (p <.05). Leukocyte 8-hydroxy-2'-deoxyguanosine (8-OHdG) was unaffected by exercise. Vitamin E decreased peak CK in young men, while in older men it decreased resting levels of iPF(2alpha) and suppressed the 24 h postexercise increases in iPF(2alpha) (p <.05). Thus, vitamin E supplementation induced modest changes eccentric exercise-induced oxidative stress, although differentially between the young and older subjects, while age had no direct influence on these responses among this group of physically fit subjects.
authors.affiliation: Nutrition, Exercise Physiology, and Sarcopenia Laboratory, Tufts University, Boston, MA 02111, USA.
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Sacheck
authors.forename: Paul E
authors.initials: PE
authors.lastname: Milbury
authors.forename: Joseph G
authors.initials: JG
authors.lastname: Cannon
authors.forename: Ronenn
authors.initials: R
authors.lastname: Roubenoff
authors.forename: Jeffrey B
authors.initials: JB
authors.lastname: Blumberg
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology*; D000975:Antioxidants / Q000494:pharmacology*; D015415:Biomarkers / Q000032:analysis*; D003402:Creatine Kinase / Q000097:blood; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D019587:Dietary Supplements; D004311:Double-Blind Method; D015444:Exercise; D028441:F2-Isoprostanes / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D028441:F2-Isoprostanes; D014810:Vitamin E; D008315:Malondialdehyde; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003402:Creatine Kinase; D003849:Deoxyguanosine