[Valoration of the FAS in the contralateral testis after unilateral testicular torsion. Experimental study in rats].
The role the FAS and BCL-2 in the apoptosis of testicular cells in the contralateral testis after unilateral testicular torsion, was investigated. We compared with control group. These experiments were performed in male Wistar rats prepuberal old. FAS and BCL-2 determination is realized in cells cultures of contralateral testis. Flow cytometry and immunohistochemistry studies, using a FAS and BCL-2 specific monoclonal antibody, were utilized to value FAS y BCL-2 expression on testiculaires cells following unilateral testicular torsion. We observed an increase of expression of FAS and decrease of BCL-2 in the contralateral testis in comparison with control group. The present results may indicate that the expression of this molecules is implicated in cellular apoptosis.
Paredes Esteban
R M
RM
Unidad de Investigación, Hospital Universitario Reina Sofía, Córdoba.
Ramírez Chamond
R
R
Carracedo Añón
J
J
Rodríguez Portillo
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D017209:Apoptosis; D018929:Cell Culture Techniques; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D008297:Male; D023421:Models, Animal; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013086:Spermatic Cord Torsion / Q000378:metabolism* / Q000473:pathology; D013737:Testis / Q000378:metabolism* / Q000473:pathology; D019014:fas Receptor / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D019253:Proto-Oncogene Proteins c-bcl-2; D019014:fas Receptor