Neuroprotective effect of antioxidants on ischaemia and reperfusion-induced cerebral injury.
The present study is designed to investigate the effect of dietary flavanoid rutin, micronutrient selenium and garlic oil on ischaemia and reperfusion-induced cerebral injury. Global cerebral ischaemia was induced by occluding right and left common carotid arteries for 10min followed by reperfusion for 24h. Cerebral infarct size was estimated using triphenyltetrazolium chloride staining. Elevated plus maze was employed to estimate short-term memory. Degree of motor incoordination was evaluated using inclined beam-walking test and lateral push test. Mitochondrial thiobarbituric acid reactive substances (TBARS) assay was employed as an index of oxidative stress. Global cerebral ischaemia followed by reperfusion produced a significant impairment in short-term memory and motor coordination and produced a notable increase in mitochondrial TBARS. Administration of rutin and garlic oil before global cerebral ischaemia markedly reduced cerebral infarct size and attenuated impairment in short-term memory and motor coordination. Administration of sodium selenite either before or after global cerebral ischaemia markedly reduced cerebral infarct size and attenuated impairment in short-term memory and motor coordination. The protective effect of rutin, sodium selenite and garlic oil was accompanied by a marked decrease in mitochondrial TBARS. On the basis of these results, it may be suggested that rutin and garlic oil administrated before cerebral ischaemia may scavenge reactive oxygen species and consequently attenuate global cerebral ischaemia and reperfusion-induced cerebral injury. Sodium selenite administrated before and after cerebral ischaemia may be neuroprotective due to its antioxidant effect.
Gupta
Ram
R
Department of Pharmaceutical Sciences and Drug Research, Punjabi University, 147002, Punjab, Patiala, India.
Singh
Manjeet
M
Sharma
Ajay
A
D000498:Allyl Compounds / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D002545:Brain Ischemia / Q000188:drug therapy*; D002544:Cerebral Infarction / Q000209:etiology / Q000517:prevention & control*; D003627:Data Interpretation, Statistical; D004195:Disease Models, Animal; D005260:Female; D008297:Male; D008569:Memory Disorders / Q000209:etiology / Q000517:prevention & control; D008570:Memory, Short-Term / Q000187:drug effects; D051379:Mice; D018977:Micronutrients / Q000494:pharmacology / Q000627:therapeutic use; D008928:Mitochondria / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D015427:Reperfusion Injury / Q000150:complications / Q000188:drug therapy*; D012431:Rutin / Q000494:pharmacology / Q000627:therapeutic use*; D018038:Sodium Selenite / Q000494:pharmacology / Q000627:therapeutic use*; D013440:Sulfides / Q000494:pharmacology / Q000627:therapeutic use*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000498:Allyl Compounds; D000975:Antioxidants; D018977:Micronutrients; D017382:Reactive Oxygen Species; D013440:Sulfides; D017392:Thiobarbituric Acid Reactive Substances; D012431:Rutin; C038491:allyl sulfide; D018038:Sodium Selenite