Chronic myeloid leukemia associated with B-cell chronic lymphocytic leukemia: evidence of two separate clones as shown by combined cell-sorting and fluorescence in situ hybridisation.
The simultaneous occurrence of Philadelphia positive chronic myeloid leukemia (Ph+ CML) and B-cell chronic lymphocytic leukemia (B-CLL) is a rare event which raises the possibility that the two malignant clones derive from a common, or distinct, malignant stem cells. In this study, we used combined CD19-based cell-sorting and fluorescence in situ hybridisation (FISH) to investigate whether or not the BCR-ABL fusion gene was present in the malignant B-cells of a patient who presented a Ph+ CML/B-CLL association. The CD19+ cells lacked the BCR-ABL rearrangement whereas all CD19-cells exhibited the fusion gene. This result demonstrates that B-cell transformation occurred in a Ph-B-cell subset.
Mansat-De Mas
Véronique
V
Laboratoire d'Hématologie, Pavillon Caubet, Centre Hospitalier, Universitaire Purpan, Place du Dr Baylac, 31059 Toulouse, France. demas.v@chu-toulouse.fr
Rigal-Huguet
Françoise
F
Cassar
Georges
G
Kuhlein
Emilienne
E
Laurent
Guy
G
Dastugue
Nicole
N
D000368:Aged; D018941:Antigens, CD19 / Q000032:analysis; D001402:B-Lymphocytes / Q000473:pathology; D002471:Cell Transformation, Neoplastic; D002999:Clone Cells / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis; D005434:Flow Cytometry; D016044:Fusion Proteins, bcr-abl / Q000032:analysis / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000175:diagnosis / Q000209:etiology / Q000473:pathology*; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000175:diagnosis / Q000209:etiology / Q000473:pathology*; D008297:Male; D009378:Neoplasms, Multiple Primary / Q000175:diagnosis / Q000209:etiology / Q000473:pathology*; D009360:Neoplastic Cells, Circulating
D018941:Antigens, CD19; D004273:DNA, Neoplasm; D016044:Fusion Proteins, bcr-abl