title: Identification and purification of immunogenic proteins from nonliving promastigote polyvalent Leishmania vaccine (Leishvacin ).
abstract: Immunogenic proteins from nonliving promastigote polyvalent Leishmania vaccine against American tegumentary leishmaniasis (Leishvacin ), produced by Biobr s (Biochemistry of Brazil ), Montes Claros, State of Minas Gerais, Brazil, were identified and purified by polyacrylamide electrophoresis gel and electroelution. C57BL/10 mice were vaccinated with proteins with estimated molecular weights of 42, 46, 63, 66, 73, 87, 97, and 160kDa in three doses of 30 g of each protein at 15-day intervals combined with 250 microg of Corynebacterium parvum followed by a challenge infection with 10(5) infective promastigotes from Leishmania (Leishmania) amazonensis. The ability of these proteins to induce immune response and protection was analyzed. No statistical difference was observed in the level of IFN-gamma induced by proteins in vaccinated groups in comparison with control groups. Six months after challenge infection, protection levels of 28.57; 42.86; 57.14; 42.86; 42.86, 57.14; 42.86 and 57.14% were demonstrated for each purified protein.
authors.affiliation: Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
authors.forename: Sandra Regina Afonso
authors.initials: SR
authors.lastname: Cardoso
authors.forename: João Carlos França
authors.initials: JC
authors.lastname: da Silva
authors.forename: Roberto Teodoro
authors.initials: RT
authors.lastname: da Costa
authors.forename: Wilson
authors.initials: W
authors.lastname: Mayrink
authors.forename: Maria Norma
authors.initials: MN
authors.lastname: Melo
authors.forename: Marilene Suzan Marques
authors.initials: MS
authors.lastname: Michalick
authors.forename: Ibrahim Afrânio Willi
authors.initials: IA
authors.lastname: Liu
authors.forename: Ricardo Toshio
authors.initials: RT
authors.lastname: Fujiwara
authors.forename: Evaldo
authors.initials: E
authors.lastname: Nascimento
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology; D001938:Brazil; D004195:Disease Models, Animal; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007371:Interferon-gamma / Q000096:biosynthesis; D007894:Leishmania mexicana / Q000276:immunology*; D016773:Leishmaniasis, Cutaneous / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D011425:Propionibacterium acnes / Q000276:immunology; D015800:Protozoan Proteins / Q000276:immunology*; D016052:Protozoan Vaccines / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D014611:Vaccination
chemical_list: D000953:Antigens, Protozoan; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D007371:Interferon-gamma