Redistribution and unmasking of Annexin V binding sites in apoptotic Raji cells.
The complement receptor type 2 (CR2) associates with other surface antigens and proteins and its redistribution and/or unmasking occurs through still unknown mechanism(s). The data presented demonstrate that high-density cultured CR2-positive cells undergo apoptosis and that the redistribution and unmasking of Annexin V binding sites occurs in a fashion similar to the redistribution and unmasking of CR2. Therefore, apoptotic and non apoptotic cells from the same lineage may share a similar mechanism for the exposition of neo-surface markers
di Certo
Maria Grazia
MG
Istituto di Biologia e Patologia Molecolare, CNR, V.le Marx 43, Rome 00137, Italy.
Faggioni
Alberto
A
Barile
Giuseppe
G
D017304:Annexin A5 / Q000378:metabolism*; D000954:Antigens, Surface; D017209:Apoptosis / Q000276:immunology*; D001665:Binding Sites / Q000276:immunology; D019070:Cell Lineage / Q000276:immunology*; D002462:Cell Membrane / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D008024:Ligands; D011485:Protein Binding / Q000276:immunology; D017464:Receptors, Complement 3d / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D017304:Annexin A5; D000954:Antigens, Surface; D008024:Ligands; D017464:Receptors, Complement 3d