title: Immunohistochemical demonstration of 14-3-3 sigma protein in normal human tissues and lung cancers, and the preponderance of its strong expression in epithelial cells of squamous cell lineage.
abstract: In order to confirm 14-3-3 sigma (sigma) protein distribution in human tissues, immunohistochemistry was performed using various paraffin-embedded human tissues. In normal human tissues, the strongest immunoreactivity for 14-3-3sigma protein was observed in squamous epithelia at various sites, followed by basal cells of the trachea, bronchus and basal or myoepithelial cells of various glands. Moderate to weak 14-3-3sigma immunoreactivity was seen in the epithelial cells of the alimentary tract, gall bladder, urinary tract and endometrium. In the lung, 14-3-3sigma immunoreactivity was also observed in hyperplastic type II alveolar cells and metaplastic squamous cells. Immunohistochemical study using non-small-cell lung cancers revealed that 14-3-3sigma immunoreactivity was stronger in squamous cell carcinomas than in adenocarcinomas. The present study revealed that 14-3-3sigma expression was exclusively present in various epithelial cells and had a tendency to be stronger in cells destined for squamous epithelium or differentiating toward squamous cells in human normal and neoplastic cells.
authors.affiliation: Second Department of Pathology and The First Department of Surgery, Gunma University School of Medicine, and Immuno-Biological Laboratories Co Ltd, Gunma, Japan. nakajima@med.gunma-u.ac.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakajima
authors.forename: Hanako
authors.initials: H
authors.lastname: Shimooka
authors.forename: Peng
authors.initials: P
authors.lastname: Weixa
authors.forename: Atsuki
authors.initials: A
authors.lastname: Segawa
authors.forename: Atsushi
authors.initials: A
authors.lastname: Motegi
authors.forename: Zhang
authors.initials: Z
authors.lastname: Jian
authors.forename: Norihiko
authors.initials: N
authors.lastname: Masuda
authors.forename: Munenori
authors.initials: M
authors.lastname: Ide
authors.forename: Takaaki
authors.initials: T
authors.lastname: Sano
authors.forename: Tetsunari
authors.initials: T
authors.lastname: Oyama
authors.forename: Hiroe
authors.initials: H
authors.lastname: Tsukagoshi
authors.forename: Kozue
authors.initials: K
authors.lastname: Hamanaka
authors.forename: Masahiro
authors.initials: M
authors.lastname: Maeda
mesh_terms: D048948:14-3-3 Proteins; D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D002452:Cell Count; D019070:Cell Lineage; D005092:Exonucleases / Q000378:metabolism*; D005095:Exoribonucleases; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D009363:Neoplasm Proteins / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D048948:14-3-3 Proteins; D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D005092:Exonucleases; D005095:Exoribonucleases; C413442:SFN protein, human