Effects of anesthesia (tricaine methanesulfonate, MS222) on liver biotransformation in rainbow trout (Oncorhynchus mykiss).
The effect of tricaine methanesulfonate (MS222) on rainbow trout liver biotransformation rates was investigated with a microsomal model; an in vitro preparation that can be employed with or without the use of an anaesthetic. Two experimental sets of rainbow trout microsomes were tested; one representing in vivo or surgical tricaine exposures and the other representing in vitro tissue/organ collection tricaine exposures. Microsomal incubations were performed on these two experimental groups with phenol as substrate to assess the effects of tricaine on Phase I (ring-hydroxylation) and II (glucuronidation) liver biotransformation by monitoring production of hydroquinone (HQ), catechol (CAT), and phenylglucuronide (PG). The use of a 2-h 100 mg/l exposure of tricaine for surgical anesthesia with or without 24-h recovery did not significantly (P< or =0.05) affect rates of phenol (Phase I and II) biotransformation rates; nor, did the 5-min 300 mg/l tricaine exposure for isolated organ/tissue collection significantly (P< or =0.05) affect phenol (Phase I and II) biotransformation rates. There were also no significant statistical differences (P< or =0.05) in P450 protein levels, or 7-ethoxyresorufin-O-deethylase (EROD) activity in these microsomal assays between any of the tricaine treated rainbow trout and controls.
Kolanczyk
Richard C
RC
Mid-Continent Ecology Division, US Environmental Protection Agency, 6201 Congdon Blvd, Duluth, MN 55804, USA. kolanczyk.rick@epa.gov
Fitzsimmons
Patrick N
PN
McKim
James M
JM
Erickson
Russell J
RJ
Schmieder
Patricia K
PK
D062365:Aminobenzoates / Q000494:pharmacology*; D000704:Analysis of Variance; D000777:Anesthetics / Q000494:pharmacology*; D000818:Animals; D001673:Biodegradation, Environmental / Q000187:drug effects; D002396:Catechols / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005965:Glucuronates / Q000378:metabolism; D006873:Hydroquinones / Q000378:metabolism; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008862:Microsomes, Liver / Q000187:drug effects* / Q000378:metabolism; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D019800:Phenol / Q000378:metabolism
D062365:Aminobenzoates; D000777:Anesthetics; D002396:Catechols; D005965:Glucuronates; D006873:Hydroquinones; C003636:tricaine; C025298:phenylglucuronide; D019800:Phenol; C034221:catechol; C031927:hydroquinone