[Effects of c9, t11-conjugated linoleic acid on the metastasis of human gastric carcinoma cell line].
In order to study the effects of c9, t11-conjugated linoleic acid on the invasion of human gastric carcinoma cell line(SGC-7901) and explore its possible mechanism, the five levels of CLA in medium were designed as 0, 25, 50, 100 and 200 mmol/L respectively. After SGC-7901 were treated with c9, t11-CLA for 24 hours, the reconstituted basement membrane invasion assay was used to evaluate invasive ability of cancer cells. The type IV collagenase was assessed by PAGE substrate zymography and the expression of TIMP-1 and TIMP-2 mRNA was measured by RT-PCR. The result displayed that c9, t11-CLA could inhibit the reconstituted basement memebrane invasion, reduce type IV collagenase activities in the serum-free supernatant of SGC-7901 cells and induce TIMP-1 and TIMP-2 mRNA expression in SGC-7901 cell line. Therefore, it is suggested that CLA could inhibit the reconstituted basement membrane invasion of SGC-7901 cells and the anti-invasion action of c9, t11-CLA might be associated with the reduction of type IV collagenase activity in tumor cell culture supernatant and induction of tumor cell TIMP-1 and TIMP-2 mRNA expression.
Yang
Yanmei
Y
Public Health School of Harbin Medical University, Harbin 150001, China.
Chen
Bingqing
B
Xue
Yingben
Y
Zheng
Yumei
Y
D000230:Adenocarcinoma / Q000473:pathology; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000473:pathology; D045744:Cell Line, Tumor; D017364:Collagenases; D006801:Humans; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D009361:Neoplasm Invasiveness / Q000517:prevention & control*; D009362:Neoplasm Metastasis / Q000517:prevention & control; D012333:RNA, Messenger / Q000096:biosynthesis; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000096:biosynthesis / Q000235:genetics; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000096:biosynthesis / Q000235:genetics
D000970:Antineoplastic Agents; D044243:Linoleic Acids, Conjugated; D012333:RNA, Messenger; D019715:Tissue Inhibitor of Metalloproteinase-1; D019716:Tissue Inhibitor of Metalloproteinase-2; D017364:Collagenases; D020778:Matrix Metalloproteinase 2