Multilocus sequence typing system for group B streptococcus.
A multilocus sequence typing (MLST) system was developed for group B streptococcus (GBS). The system was used to characterize a collection (n = 152) of globally and ecologically diverse human strains of GBS that included representatives of capsular serotypes Ia, Ib, II, III, V, VI, and VIII. Fragments (459 to 519 bp) of seven housekeeping genes were amplified by PCR for each strain and sequenced. The combination of alleles at the seven loci provided an allelic profile or sequence type (ST) for each strain. A subset of the strains were characterized by restriction digest patterning, and these results were highly congruent with those obtained with MLST. There were 29 STs, but 66% of isolates were assigned to four major STs. ST-1 and ST-19 were significantly associated with asymptomatic carriage, whereas ST-23 included both carried and invasive strains. All 44 isolates of ST-17 were serotype III clones, and this ST appeared to define a homogeneous clone that was strongly associated with neonatal invasive infections. The finding that isolates with different capsular serotypes had the same ST suggests that recombination occurs at the capsular locus. A web site for GBS MLST was set up and can be accessed at http://sagalactiae.mlst.net. The GBS MLST system offers investigators a valuable typing tool that will promote further investigation of the population biology of this organism.
Jones
Nicola
N
Nuffield Department of Clinical Laboratory Sciences, Institute for Molecular Medicine, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom. nicola.jones@ndcls.ox.ac.uk
Bohnsack
John F
JF
Takahashi
Shinji
S
Oliver
Karen A
KA
Chan
Man-Suen
MS
Kunst
Frank
F
Glaser
Philippe
P
Rusniok
Christophe
C
Crook
Derrick W M
DW
Harding
Rosalind M
RM
Bisharat
Naiel
N
Spratt
Brian G
BG
D000328:Adult; D000483:Alleles; D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D019295:Computational Biology; D006801:Humans; D007231:Infant, Newborn; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D013290:Streptococcal Infections / Q000382:microbiology; D013292:Streptococcus agalactiae / Q000145:classification* / Q000235:genetics
D001426:Bacterial Proteins