Microbial production of 2-deoxyribose 5-phosphate from acetaldehyde and triosephosphate for the synthesis of 2'-deoxyribonucleosides.
2-Deoxyribose 5-phosphate was produced from acetaldehyde and dihydroxyacetone phosphate via D-glyceraldehyde 3-phosphate by Klebsiella pneumoniae B-4-4 through deoxyriboaldolase- and triosephosphate isomerase-catalyzing reactions. Under the optimum conditions, 98.7 mM 2-deoxyribose 5-phosphate was produced from 200 mM acetaldehyde and 117 mM dihydroxyacetone phosphate in 2 h with a molar yield of 84%. The 2-deoxyriobse 5-phosphate produced was directly transformed to 2'-deoxyribonucleoside by phosphopentomutase- and nucleoside phosphorylase-catalyzing reactions.
Ogawa
Jun
J
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.
Saito
Kyota
K
Sakai
Takafumi
T
Horinouchi
Nobuyuki
N
Kawano
Takako
T
Matsumoto
Seiichiro
S
Sasaki
Mie
M
Mikami
Yoichi
Y
Shimizu
Sakayu
S
D000079:Acetaldehyde / Q000378:metabolism; D003853:Deoxyribonucleosides / Q000096:biosynthesis*; D007711:Klebsiella pneumoniae / Q000378:metabolism*; D010430:Pentosyltransferases / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism; D012267:Ribosemonophosphates / Q000096:biosynthesis*; D013403:Sugar Phosphates / Q000378:metabolism; D014306:Trioses / Q000378:metabolism
D003853:Deoxyribonucleosides; D012267:Ribosemonophosphates; D013403:Sugar Phosphates; D014306:Trioses; C058245:2-deoxyribose 5-phosphate; D010430:Pentosyltransferases; C067995:nucleoside phosphorylase; D010770:Phosphotransferases; C022375:phosphopentomutase; D000079:Acetaldehyde