Sequential protein association with nascent 60S ribosomal particles.
Ribosome biogenesis in eukaryotes depends on the coordinated action of ribosomal and nonribosomal proteins that guide the assembly of preribosomal particles. These intermediate particles follow a maturation pathway in which important changes in their protein composition occur. The mechanisms involved in the coordinated assembly of the ribosomal particles are poorly understood. We show here that the association of preribosomal factors with pre-60S complexes depends on the presence of earlier factors, a phenomenon essential for ribosome biogenesis. The analysis of the composition of purified preribosomal complexes blocked in maturation at specific steps allowed us to propose a model of sequential protein association with, and dissociation from, early pre-60S complexes for several preribosomal factors such as Mak11, Ssf1, Rlp24, Nog1, and Nog2. The presence of either Ssf1 or Nog2 in complexes that contain the 27SB pre-rRNA defines novel, distinct pre-60S particles that contain the same pre-rRNA intermediates and that differ only by the presence or absence of specific proteins. Physical and functional interactions between Rlp24 and Nog1 revealed that the assembly steps are, at least in part, mediated by direct protein-protein interactions.
Saveanu
Cosmin
C
Génétique des Interactions Macromoléculaires, CNRS-URA2171. PT Protéomique, Institut Pasteur, 75724 Paris Cedex 15, France.
Namane
Abdelkader
A
Gleizes
Pierre-Emmanuel
PE
Lebreton
Alice
A
Rousselle
Jean-Claude
JC
Noaillac-Depeyre
Jacqueline
J
Gas
Nicole
N
Jacquier
Alain
A
Fromont-Racine
Micheline
M
D000595:Amino Acid Sequence; D015152:Blotting, Northern; D002466:Cell Nucleolus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D005816:Genetic Complementation Test; D005838:Genotype; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D009841:Oligonucleotides / Q000737:chemistry; D016366:Open Reading Frames; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D012335:RNA, Ribosomal / Q000378:metabolism; D012270:Ribosomes / Q000737:chemistry* / Q000648:ultrastructure; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013395:Sucrose / Q000494:pharmacology; D013997:Time Factors
D009687:Nuclear Proteins; D009841:Oligonucleotides; D012335:RNA, Ribosomal; C114037:SSF1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D013395:Sucrose