title: HPLC determination of (+)-pseudoephedrine and (-)-ephedrine in Japanese herbal medicines containing Ephedra herb using solid-phase extraction.
abstract: We developed a rapid and simple HPLC method combined with solid-phase extraction (SPE) for quantitative analysis of (+)-pseudoephedrine (PEP) and (-)-ephedrine (EP) in Japanese herbal (Kampo) medicines such as Kakkon-to, Sho-seiryu-to, Goshaku-san and Bofu-tsusho-san. SPE was performed on TOYOPAK IC-SP M containing propylsulfonic groups. Determination of PEP and EP was carried out using ion-pair reversed-phase HPLC with sodium dodecyl sulfate. N-Benzyldiethylamine was used as an internal standard. The analytical procedure was validated with regard to specificity, linearity, accuracy, and precision. These data suggest that the analytical method developed in this study is useful for quantitative analysis of PEP and EP in various formulations of Kampo medicine containing Ephedra herb.
authors.affiliation: Healthcare Research Institute, Wakunaga Pharmaceutical Co., Ltd, Hiroshima, Japan. ichikawa_m@wakunaga.co.jp
authors.forename: Makoto
authors.initials: M
authors.lastname: Ichikawa
authors.forename: Manabu
authors.initials: M
authors.lastname: Udayama
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Imamura
authors.forename: Sumihiro
authors.initials: S
authors.lastname: Shiraishi
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Matsuura
mesh_terms: D002851:Chromatography, High Pressure Liquid; D029789:Ephedra / Q000737:chemistry*; D004809:Ephedrine / Q000032:analysis*; D029001:Herbal Medicine / Q000592:standards*; D007536:Isomerism; D007564:Japan; D020835:Medicine, Kampo; D010936:Plant Extracts / Q000737:chemistry / Q000592:standards
chemical_list: D010936:Plant Extracts; D004809:Ephedrine