Bioactive crude plant seed extracts from the NCAUR oilseed repository.
Over four-hundred crude extracts from 202 plant species distributed among 131 plant families were evaluated for their bioactivity against brine shrimp (Artemia salina). Activity was determined for both the organic (CH2Cl2:MeOH) and aqueous extracts against A. salina in a 96 well-plate assay. Of the greater than four-hundred extracts tested, 21 organic and 6 aqueous extracts demonstrated potent cytotoxic activity (LC50 = < 100 microg/ml). Three of these organic extracts (Crateva religiosa, Diospyros dichrophylla, and Olax subscorpioidea) were chosen for chemical investigations due to their high activity and a lack of prior investigations. Chemical analysis of these extracts resulted in the isolation of oleanolic acid (1) and 4-epi-hederagenin (2) from C. religiosa, isodiospyrin (3) from D. dichrophylla, and santalbic acid (4) from O. subscorpioidea.
Cantrell
C L
CL
U.S. Department of Agriculture, Agricultural Research Service, National Center for Agricultural Utilization Research, Peoria, Illinois, USA. c.cantrell@hauser.com
Berhow
M A
MA
Phillips
B S
BS
Duval
S M
SM
Weisleder
D
D
Vaughn
S F
SF
D000818:Animals; D001156:Artemia / Q000187:drug effects; D031483:Capparaceae; D029788:Diospyros; D007928:Lethal Dose 50; D031656:Olacaceae; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal; D012639:Seeds
D010936:Plant Extracts