[In silicon cloning of the human TECTB gene].
The coding sequence of the mouse Tectb and chick Tectb gene were subjected to Blastn searching against the human dbEST and Htgs in NCBI. One BAC clone sequence(GenBank: AL157786) was obtained, which shows high homology to the two genes. We predicted the exons and introns in the homologous region of AL157786 using GENSCAN, MZEF and Blast 2 sequence program, and then assembled the predicted exons into the coding sequence of the human TECTB. The open reading frame of human TECTB gene is 990 bp composed of ten exons, which encodes a protein of 329 amino acids. Human TECTB gene shows 88.1% identity in 990 bp overlap with that of the mouse Tectb gene and the predicted polypeptide shows 94.2% identity in 329 as with the mouse beta-tectorin. The TECTB gene was mapped to human chromosome 10q25 by electronic-PCR.
Zhang
Hua-Li
HL
National Laboratory of Medical Genetics of China, Central South University, Changsha 410078, China. zhl72@yahoo.com.cn
Deng
Hao
H
Zhang
Rui-Fang
RF
Xia
Kun
K
Xia
Jia-Hui
JH
D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10 / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D030561:Databases, Nucleic Acid; D005091:Exons; D016326:Extracellular Matrix Proteins / Q000235:genetics*; D058851:GPI-Linked Proteins; D006801:Humans; D007438:Introns; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D016415:Sequence Alignment / Q000379:methods*; D017386:Sequence Homology, Amino Acid; D026801:Synteny
D018076:DNA, Complementary; D016326:Extracellular Matrix Proteins; D058851:GPI-Linked Proteins; D008565:Membrane Proteins; C093123:TECTB protein, human