Drosophila MCM10 interacts with members of the prereplication complex and is required for proper chromosome condensation.
Mcm10 is required for the initiation of DNA replication in Saccharomyces cerevisiae. We have cloned MCM10 from Drosophila melanogaster and show that it complements a ScMCM10 null mutant. Moreover, Mcm10 interacts with key members of the prereplication complex: Mcm2, Dup (Cdt1), and Orc2. Interactions were also detected between Mcm10 and itself, Cdc45, and Hp1. RNAi depletion of Orc2 and Mcm10 in KC cells results in loss of DNA content. Furthermore, depletion of Mcm10, Cdc45, Mcm2, Mcm5, and Orc2, respectively, results in aberrant chromosome condensation. The condensation defects observed resemble previously published reports for Orc2, Orc5, and Mcm4 mutants. Our results strengthen and extend the argument that the processes of chromatin condensation and DNA replication are linked.
Christensen
Tim W
TW
Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853-2703, USA.
Tye
Bik K
BK
D000818:Animals; D002453:Cell Cycle / Q000502:physiology; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone; D002875:Chromosomes / Q000378:metabolism*; D004261:DNA Replication / Q000502:physiology; D004268:DNA-Binding Proteins / Q000378:metabolism; D004330:Drosophila / Q000235:genetics* / Q000378:metabolism; D029721:Drosophila Proteins; D005656:Fungal Proteins / Q000378:metabolism; D064110:Minichromosome Maintenance Proteins; D051738:Origin Recognition Complex; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins
D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D005656:Fungal Proteins; C489612:MCM10 protein, Drosophila; C076365:MCM10 protein, S cerevisiae; C497215:ORC2 protein, S cerevisiae; C497216:Orc2 protein, Drosophila; D051738:Origin Recognition Complex; D029701:Saccharomyces cerevisiae Proteins; C069199:MCM2 protein, S cerevisiae; D064110:Minichromosome Maintenance Proteins