title: Direct detection of potassium cations bound to G-quadruplex structures by solid-state 39K NMR at 19.6 T.
abstract: We report solid-state 39K NMR detection of the K+ ions bound to three G-quadruplex structures formed by self-assembly of 5'-tert-butyl-dimethylsilyl-2',3'-O-isopropylidene guanosine, guanosine, and guanosine 5'-monophosphate. The 39K NMR spectra clearly show different spectral signatures for K+ ions inside the G-quadruplex channel and for K+ ions bound to the phosphate groups. Solid-state 39K NMR spectra for hydrated K salts of adenosine 2'-monophosphate and adenosine 5'-diphosphate are also reported.
authors.affiliation: Department of Chemistry, Queen's University, Kingston, Ontario, Canada K7L 3N6. gangwu@chem.queensu.ca
authors.forename: Gang
authors.initials: G
authors.lastname: Wu
authors.forename: Alan
authors.initials: A
authors.lastname: Wong
authors.forename: Zhehong
authors.initials: Z
authors.lastname: Gan
authors.forename: Jeffery T
authors.initials: JT
authors.lastname: Davis
mesh_terms: D002414:Cations, Monovalent; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D054856:G-Quadruplexes; D006151:Guanosine / Q000031:analogs & derivatives* / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D009690:Nucleic Acid Conformation; D011188:Potassium / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism
chemical_list: D002414:Cations, Monovalent; D006151:Guanosine; D004247:DNA; D011188:Potassium