title: Isolation of human polymorphonuclear leukocytes (granulocytes) from a leukocyte-rich fraction.
abstract: Human peripheral blood polymorphonuclear leukocytes (PMNs) or granulocytes from a leukocyte-rich plasma (LRP) are banded at an interface between two layers of iodixanol. If the denser layer of iodixanol is omitted the PMNs may alternatively be pelleted. The procedure can be adapted to blood from other species by small changes to the density of the two iodixanol layers. The method works optimally with EDTA- or citrate-anticoagulated blood.
authors.affiliation: School of Biomolecular Sciences, Liverpool John Moores University. john@jgrescon.fsbusiness.co.uk
authors.forename: John M
authors.initials: JM
authors.lastname: Graham
mesh_terms: D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient / Q000379:methods; D006098:Granulocytes / Q000166:cytology*; D006801:Humans; D007962:Leukocytes / Q000737:chemistry* / Q000166:cytology*; D014283:Triiodobenzoic Acids / Q000737:chemistry
chemical_list: D014283:Triiodobenzoic Acids; C044834:iodixanol