title: The effects of a triclosan/copolymer dentifrice on oral bacteria including those producing hydrogen sulfide.
abstract: A clinical procedure was developed to examine the effects of short-term and extended use of a triclosan/copolymer dentifrice and a commercial fluoride dentifrice on oral bacteria, including those producing hydrogen sulfide. Healthy adults volunteered for this double-blind, crossover design clinical study and provided saliva samples for culturing on enriched and indicator media to enumerate all salivary bacteria and those producing hydrogen sulfide (odorigenic), respectively. Subjects brushed with an assigned dentifrice for 7 d and were sampled on day 8 to assess the long-term effects on bacteria. Extended use of the triclosan/copolymer dentifrice resulted in a 49% and 66% reduction of salivary and odorigenic bacteria, respectively, compared with the fluoride dentifrice. To examine short-term effects, subjects subsequently brushed with their assigned dentifrice and were sampled at 2 h and 4 h post-brushing. At 2 h and 4 h post-brushing, the triclosan/copolymer dentifrice resulted in a 62% and 52% decrease for salivary bacteria and 79% and 72% decrease for odorigenic bacteria, respectively, vs. the fluoride dentifrice. The results indicate a significant decrease of all salivary bacteria and hydrogen sulfide-producing odorigenic bacteria following use of the triclosan/copolymer dentifrice and explain previous results on the efficacy of this dentifrice on oral malodor.
authors.affiliation: Colgate-Palmolive Company, 090 River Road, Piscataway, NJ 08855, USA. prem_sreenivasan@colpal.com
authors.forename: Prem
authors.initials: P
authors.lastname: Sreenivasan
mesh_terms: D000328:Adult; D000368:Aged; D000891:Anti-Infective Agents, Local / Q000494:pharmacology / Q000627:therapeutic use; D002167:Campylobacter / Q000187:drug effects / Q000378:metabolism; D015169:Colony Count, Microbial; D045424:Complex Mixtures; D018592:Cross-Over Studies; D003802:Dentifrices / Q000494:pharmacology / Q000627:therapeutic use*; D004311:Double-Blind Method; D005260:Female; D005459:Fluorides; D005673:Fusobacterium / Q000187:drug effects / Q000378:metabolism; D006209:Halitosis / Q000188:drug therapy* / Q000382:microbiology*; D006801:Humans; D006862:Hydrogen Sulfide / Q000378:metabolism; D008297:Male; D008298:Maleates / Q000494:pharmacology / Q000627:therapeutic use; D008875:Middle Aged; D010462:Peptostreptococcus / Q000187:drug effects / Q000378:metabolism; D011095:Polyethylenes / Q000494:pharmacology / Q000627:therapeutic use; D018720:Prevotella / Q000187:drug effects / Q000378:metabolism; D012463:Saliva / Q000382:microbiology; D012824:Silicic Acid; D014100:Toothpastes; D014260:Triclosan / Q000494:pharmacology / Q000627:therapeutic use; D014678:Veillonella / Q000187:drug effects / Q000378:metabolism
chemical_list: D000891:Anti-Infective Agents, Local; D045424:Complex Mixtures; D003802:Dentifrices; D008298:Maleates; D011095:Polyethylenes; D014100:Toothpastes; C546060:hydrated silica gel-based toothpaste; D012824:Silicic Acid; D014260:Triclosan; C043105:poly(methyl vinyl ether-co-maleic anhydride); D005459:Fluorides; D006862:Hydrogen Sulfide