Stability of diacylglycerol acyltransferase in dehydrated bovine muscle tissue.
Meaningful estimates of diacylglycerol acyltransferase (EC 2.3.1.20) activity in different tissue samples require effective, unbiased methods of sample storage. Samples of the pars costalis diaphragmatis muscle (skirt muscle of the diaphragm) were obtained from 18- to 20-month-old cattle and assayed for microsomal protein content and diacylglycerol acyltransferase activity after having been stored under various conditions as dissected tissue or microsomes prepared from dissected tissue. There was relative enrichment of diacylglycerol acyltransferase specific activity (p<0.05) when samples prepared from the pars costalis diaphragmatis muscle were dehydrated and stored for 2 weeks, as compared to the control condition (in which the microsome fraction was prepared from fresh pars costalis diaphragmatis muscle and assayed immediately). The results suggested that dehydration was an effective method of storage for bovine muscle samples destined for estimation of the microsomal diacylglycerol acyltransferase activity. The dehydration approach for preparing samples for analysis of diacylglycerol acyltransferase activity might also prove useful to investigators who are interested in obtaining reliable estimates of the activity of other enzymes in tissue samples.
Huff
Phillip W
PW
Department of Chemistry and Biochemistry, University of Lethbridge, Lethbridge, Alberta, Canada T1K 3M4.
Lozeman
Fred J
FJ
Kazala
E Chris
EC
Prozniak
Cara
C
Wegner
Jochen
J
Deng
Jingti
J
Laroche
André
A
Mir
Priya S
PS
Aalhus
Jennifer
J
Weselake
Randall J
RJ
D000217:Acyltransferases / Q000737:chemistry* / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D003890:Desiccation; D051048:Diacylglycerol O-Acyltransferase; D003964:Diaphragm / Q000201:enzymology*; D004795:Enzyme Stability; D011487:Protein Conformation
D000217:Acyltransferases; D051048:Diacylglycerol O-Acyltransferase