title: Kinetic parameters of continuous cultures of Bacillus thermoleovorans sp. A2 degrading phenol at 65 degrees C.
abstract: In this paper, we report on the kinetics of phenol degradation and cell growth in continuous cultures of suspended cells of Bacillus thermoleovorans sp. A2 at 65 degrees C. A high yield coefficient of Y(x/s)=0.84 g cell dry weight g(-1) phenol was measured at a dilution rate of 0.5 h(-1). At the same dilution rate the coefficient for maintenance metabolism (m(s)) was determined to be 0.045 g phenol g(-1) cell dry weight h(-1). The maximal growth rate (wash-out) determined at a phenol inlet concentration of 188 mg l(-1) was 0.9 h(-1). Up to 7 g phenol l(-1) per day were degraded in a continuously operated 2-l stirred tank reactor with suspended cells (feed concentration 660 mg l(-1)). Additionally, yield coefficients for oxygen and ammonium are reported.
authors.affiliation: Laboratory of Chemical Engineering and Industrial Chemistry, ETH Zürich, Hönggerberg, CH-8093 Zürich, Switzerland. feitkenhauer@tech.ethz.ch
authors.forename: Heiko
authors.initials: H
authors.lastname: Feitkenhauer
authors.forename: Stephanie
authors.initials: S
authors.lastname: Schnicke
authors.forename: Rudolf
authors.initials: R
authors.lastname: Müller
authors.forename: Herbert
authors.initials: H
authors.lastname: Märkl
mesh_terms: D001407:Bacillus / Q000254:growth & development* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology*; D003470:Culture Media; D006358:Hot Temperature; D007700:Kinetics; D008903:Minerals; D008954:Models, Biological; D010100:Oxygen / Q000378:metabolism; D019800:Phenol / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D014865:Waste Disposal, Fluid / Q000379:methods*
chemical_list: D003470:Culture Media; D008903:Minerals; D000644:Quaternary Ammonium Compounds; D019800:Phenol; D010100:Oxygen