Effect of the isocoumarin paepalantine on the luminol and lucigenin amplified chemiluminescence of rat neutrophils.
Paepalantine (9,10-dihydroxy-5,7-dimethoxy-1H-naphto(2,3c)pyran-1-one), a natural isocoumarin isolated from the capitula of Paepalanthus bromelioides (Eriocaulaceae), was assessed for its effect on the respiratory burst (zymosan-stimulated luminol-enhanced chemiluminescence and PMA-stimulated lucigenin-enhanced chemiluminescence) of polymorphonuclear neutrophils in vitro. Special attention was devoted to establishing the IC(50) for neutrophils. Paepalantine was able to decrease luminol and lucigenin chemiluminescence, reflecting an inhibitory effect on the respiratory burst, with an ED(50) of 0.44+/-0.05 and 0.84+/-0.15 microg/ml, respectively. A cell-free system was performed with paepalantine on myeloperoxidase/H(2)O(2) and myeloperoxidase/H(2)O(2)/Cl(-) systems. Paepalantine inhibited luminol oxidation in both systems. This inhibition was related to the interaction of paepalantine-myeloperoxidase and its scavenger effect on HOCl.
Kitagawa
Rodrigo Rezende
RR
Instituto de Química de Araraquara, Universidade Estadual Paulista Júlio de Mesquita Filho, Rua Prof. Francisco Degni, s/n, CP 355, 14801-970 Araraquara, SP, Brazil.
Raddi
Maria Stella Gonçalves
MS
Khalil
Najeh Maissar
NM
Vilegas
Wagner
W
da Fonseca
Luiz Marcos
LM
D000166:Acridines; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D003374:Coumarins / Q000494:pharmacology*; D006997:Hypochlorous Acid / Q000494:pharmacology; D066298:In Vitro Techniques; D049934:Isocoumarins; D008163:Luminescent Measurements; D008165:Luminol; D008297:Male; D009504:Neutrophils / Q000187:drug effects* / Q000201:enzymology; D009195:Peroxidase / Q000378:metabolism; D051381:Rats
D000166:Acridines; D000975:Antioxidants; D003374:Coumarins; D049934:Isocoumarins; C108025:paepalantine; C033472:10,10'-dimethyl-9,9'-biacridinium; D008165:Luminol; D006997:Hypochlorous Acid; D009195:Peroxidase