title: The PrP genotype of sheep of the improved Valachian breed.
abstract: In a worldwide majority of sheep breeds an excessive susceptibility to scrapie associated with the PrP gene alleles coding for valine (V; at the 136 codon) and glutamine (Q; at the 171 codon) (e.g., VRQ/VRQ, VRQ/ARQ, or ARQ/ARQ) was demonstrated. Particularly the PrPVRQ allele is closely associated with the high-risk development of the disease; the PrPARQ allele can also fulfill this function but under certain limited conditions. Polymorphism in the PrP gene sequences (conclusively related to the increased susceptibility of sheep to scrapie) of improved Valachian sheep from two Slovak regions, Orava and Spis, was determined. Examination of 735 sheep showed that ARR/ARQ was the most frequent genotype (45.2%). High-risk genotypes were determined in 32.4% of sheep (ARQ/ARQ 19.3, ARR/VRQ 9.0, ARR/VRQ 3.5, VRQ/VRQ 0.3, ARR/VRR 0.3). Low-risk genotypes were found in 67.7% of sheep (ARR/ARQ 45.2, ARR/ARR 10.9, ARR/AHQ 5.7, ARQ/ARQ 4.9, AHQ/AHQ 0.7, ARR/AHR 0.3). Despite the geographically distant flocks of improved Valachian sheep investigated no difference in the occurrence of individual PrP genotypes was observed.
authors.affiliation: Institute of Microbiology and Immunology, University of Veterinary Medicine, 041 81 Kosice, Slovakia.
authors.forename: L
authors.initials: L
authors.lastname: Tkáciková
authors.forename: E
authors.initials: E
authors.lastname: Hanusovská
authors.forename: M
authors.initials: M
authors.lastname: Novák
authors.forename: M
authors.initials: M
authors.lastname: Arvayová
authors.forename: I
authors.initials: I
authors.lastname: Mikula
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D001947:Breeding; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D008297:Male; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011328:Prions / Q000145:classification* / Q000235:genetics*; D012608:Scrapie / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012756:Sheep; D012757:Sheep Diseases / Q000235:genetics*; D018154:Slovakia
chemical_list: D011328:Prions