[Differentiation of pluripotent embryonic stem cells in peritoneal cavity of irradiated mice].
We studied the behavior and differentiation of pluripotent embryonic stem cells of R1 mice in vivo. Undifferentiated embryonic stem cells and differentiating embryoid bodies grafted in abdominal cavity of the irradiated mice were shown to form tumors with derivatives of all germinal layers. Cells of the embryoid bodies form tumors two weeks after the grafting, while the undifferentiated embryonic stem cells form tumors only by week three.
Gordeeva
O F
OF
Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, ul. Vavilova 26, Moscow, 119991 Russia. olgagordeeva@yandex.ru
Manuilova
E S
ES
Paiushina
O V
OV
Nikonova
T M
TM
Grivennikov
I A
IA
Khrushchev
N G
NG
D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000528:radiation effects; D002454:Cell Differentiation / Q000528:radiation effects*; D002471:Cell Transformation, Neoplastic / Q000473:pathology* / Q000528:radiation effects; D005720:Gamma Rays; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D039904:Pluripotent Stem Cells / Q000473:pathology* / Q000528:radiation effects; D033581:Stem Cell Transplantation; D013724:Teratoma / Q000473:pathology*; D014916:Whole-Body Irradiation