title: Genetic effects on urinary 1-hydroxypyrene levels in a Korean population.
abstract: Urinary 1-hydroxypyrene (1-OHP) has been used as a biomarker for assessing the level of exposure to environmental carcinogenic polycyclic aromatic hydrocarbons (PAHs). In order to perform the appropriate biological monitoring for examining the level of exposure to PAHs, this study investigated whether or not genetic polymorphisms of the metabolic enzymes, which might be involved in the metabolism of pyrene, affected the urinary 1-OHP levels in a population of 661 Koreans (male, 63%; female, 37%; mean age, 36.5 +/- 11.1 years) who were not occupationally exposed to PAHs. Urinary 1-OHP was detected in 76% of the subjects (range 0.001-3.8 micro g/l). Among the physical and lifestyle factors, cigarette-smoking was found to be associated with the urinary 1-OHP levels (P < 0.05). After adjusting for these factors, we found that the GSTT1 genotypes affected the urinary 1-OHP levels, i.e. the GSTT1 present subjects had approximately 1.5 times the urinary 1-OHP level than the GSTT1 null subjects (P < 0.05). In the case of the subjects who were also GSTM1 null, this trend became stronger, i.e. the GSTT1 present subjects had approximately 2 times the urinary 1-OHP level (P < 0.01). However, the genetic polymorphism of the other metabolic enzymes, cytochrome P-450 (CYP)1A1, CYP1B1 and GSTM1 alone, did not affect the urinary 1-OHP level. Therefore, this study suggests that the GSTT1 genetic polymorphism has the potential to affect the biological monitoring of PAHs with urinary 1-OHP, and might act as a genetic factor in PAH-related toxicity.
authors.affiliation: Department of Preventive Medicine/Cancer Research Institute, Seoul National University, Seoul, Korea.
authors.forename: Mihi
authors.initials: M
authors.lastname: Yang
authors.forename: Jae-Yeon
authors.initials: JY
authors.lastname: Jang
authors.forename: Soyeon
authors.initials: S
authors.lastname: Kim
authors.forename: Su-Man
authors.initials: SM
authors.lastname: Lee
authors.forename: Seong-Sil
authors.initials: SS
authors.lastname: Chang
authors.forename: Hae-Kwan
authors.initials: HK
authors.lastname: Cheong
authors.forename: Eunil
authors.initials: E
authors.lastname: Lee
authors.forename: Daehee
authors.initials: D
authors.lastname: Kang
authors.forename: Ho
authors.initials: H
authors.lastname: Kim
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Kawamoto
authors.forename: Hyoung Doo
authors.initials: HD
authors.lastname: Shin
mesh_terms: D000328:Adult; D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics; D003062:Codon; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics; D065633:Cytochrome P-450 CYP1B1; D005260:Female; D005982:Glutathione Transferase / Q000235:genetics; D006801:Humans; D007723:Korea; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011721:Pyrenes / Q000378:metabolism*
chemical_list: D001189:Aryl Hydrocarbon Hydroxylases; D003062:Codon; D019363:Cytochrome P-450 CYP1A1; D065633:Cytochrome P-450 CYP1B1; D005982:Glutathione Transferase; D011721:Pyrenes; C585496:CYP1B1 protein, human; C413545:glutathione S-transferase T1; C033146:1-hydroxypyrene