title: Expression of insulin-like growth factor 1 and insulin-like growth factor 1 receptor and its intervention by interleukin-10 in experimental hepatic fibrosis.
abstract: OBJECTIVE
  To study the expression of IGF-1 and IGF-1R and its intervention by interleukin-10 in the course of experimental hepatic fibrosis.
  
  
  METHODS
  Hepatic fibrosis was induced in rats by carbon tetrachloride intoxication and liver specimens were taken from the rats administered CCl4 with or without IL-10 treatment and the animals of the control group. Immunoreactivities for insulin-like growth factor-1 (IGF-1) and IGF-1 receptor(IGF-1R) were demonstrated by immunohistochemistry, and their intensities were evaluated in different animal groups.
  
  
  RESULTS
  The positive levels for IGF-1 and IGF-1R were increased with the development of hepatic fibrosis, with the positive signals localized in cytoplasm and/or at the plasmic membrane of hepatocytes. The positive signals of IGF-1 and IGF-1R were observed more frequently (P<0.01) in the CCl4-treated group (92.0 % and 90.0 %) compared to those in the control group. The positive signals decreased significantly (P<0.05) in IL-10-treated group. The responses in IGF-1 and IGF-1R expression correlated with the time of IL-10 treatment.
  
  
  CONCLUSIONS
  The expression of IGF-1 and IGF-1R immunoreactivities in liver tissue seems to be up-regulated during development of hepatic fibrosis induced by CCl(4), and exogenic IL-10 inhibits the responses.
authors.affiliation: Department of Gastroenterology, Affiliated Union Hospital, Fujian Medical University, Fuzhou, 350001, Fujian Province, China. drwangxz@pub6.fz.fj.cn
authors.forename: Xiao-Zhong
authors.initials: XZ
authors.lastname: Wang
authors.forename: Zhi-Xin
authors.initials: ZX
authors.lastname: Chen
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Zhang
authors.forename: Yun-Xin
authors.initials: YX
authors.lastname: Chen
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Feng-Lin
authors.initials: FL
authors.lastname: Chen
authors.forename: Yue-Hong
authors.initials: YH
authors.lastname: Huang
mesh_terms: D000818:Animals; D007150:Immunohistochemistry; D007334:Insulin-Like Growth Factor I / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D016753:Interleukin-10 / Q000494:pharmacology*; D008106:Liver Cirrhosis, Experimental / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017526:Receptor, IGF Type 1 / Q000037:antagonists & inhibitors* / Q000378:metabolism*
chemical_list: D016753:Interleukin-10; D007334:Insulin-Like Growth Factor I; D017526:Receptor, IGF Type 1