Purification of islets of Langerhans from porcine pancreas.
Flotation through a slightly hyperosmotic discontinuous gradient iodixanol achieves a much higher recovery of islets of an improved viability than the customary method using sedimentation on to a diatrizoate/polysaccharide barrier. Flotation techniques achieve an enhanced separation of the islets from any residual digestive enzymes and from acinar cells. The method has been extended to human pancreas.
Graham
John M
JM
School of Biomolecular Sciences, Liverpool John Moores University. john@jgrescon.fsbusiness.co.uk
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D007515:Islets of Langerhans / Q000166:cytology*; D013552:Swine / Q000033:anatomy & histology*