[Teratogenicity of bisphenol A on post-implanted rat and mouse embryos: an in vitro study].
In order to evaluate the developmental toxicity of BPA and to investigate the possible mechanism of it in vitro, whole embryo culture was used for the study. The embryos that removed from mother mice on gestational 8.5d (GD8.5) and mother rats on gestational 9.5d (GD9.5) were cultured immediately in centrifugal serum (ICS) with a series of concentrations of 0, 40, 60, 80, 100 mg/L of BPA for 48 h to evaluate the embryo development and morphological differentiation. The results showed that the BPA inhibited the development of embryos in vitro and there was a dose-response relationship between concentrations of BPA. At high level (> or = 60 mg/L), BPA could delayed the growth and cardiac tube differentiation of visceral yolk sac (VYS), and induced various embryo defects, including abnormal neural system, arch, optic, flexion and small limb bud, etc. It is concluded that at high level, BPA had toxicity to mice and rat embryos in vitro. The damage on structure and function of VYS might be part of teratogenic mechanisms of BPA.
Li
Yong
Y
Department of Nutrition & Food Hygiene, School of Public Health, Peking University, Beijing 100083, China.
Pei
Xinrong
X
Long
Dingxin
D
Chen
Xing
X
D000818:Animals; D001559:Benzhydryl Compounds; D046508:Culture Techniques; D004305:Dose-Response Relationship, Drug; D005314:Embryonic and Fetal Development / Q000187:drug effects; D005260:Female; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010636:Phenols / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D013723:Teratogens / Q000633:toxicity*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D010636:Phenols; D013723:Teratogens; C006780:bisphenol A; D000098826:Bisphenol A Compounds