[Purification of porcine pulmonary surfactant by CO2 supercritical fluid extraction].
OBJECTIVE
To develop a supercritical fluid extraction (SFE) method for purifying porcine pulmonary surfactant.


METHODS
An orthogonal design with 4 factors and 4 levels was used to optimize the SFE conditions.


RESULTS
The best extraction conditions were established as the following: pressure 30 MPa, temperature 50 degrees Celsius, dynamic extraction for 4 h with 0.2 ml ethanol as the modifier.


CONCLUSIONS
The new method for purifying porcine pulmonary surfactant by SFE-CO2 can cause drastic reduction of organic solvents, being convenient to use and effective to purify.
Zhao
Qian
Q
Department of Pediatrics, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China.
Feng
Zhi-chun
ZC
Du
Jiang
J
D000818:Animals; D002245:Carbon Dioxide; D010743:Phospholipids / Q000032:analysis; D011663:Pulmonary Surfactants / Q000032:analysis / Q000302:isolation & purification*; D013552:Swine; D013696:Temperature
D010743:Phospholipids; D011663:Pulmonary Surfactants; D002245:Carbon Dioxide