Consequences of reductive evolution for gene expression in an obligate endosymbiont.
The smallest cellular genomes are found in obligate symbiotic and pathogenic bacteria living within eukaryotic hosts. In comparison with large genomes of free-living relatives, these reduced genomes are rearranged and have lost most regulatory elements. To test whether reduced bacterial genomes incur reduced regulatory capacities, we used full-genome microarrays to evaluate transcriptional response to environmental stress in Buchnera aphidicola, the obligate endosymbiont of aphids. The 580 genes of the B. aphidicola genome represent a subset of the 4500 genes known from the related organism, Escherichia coli. Although over 20 orthologues of E. coli heat stress (HS) genes are retained by B. aphidicola, only five were differentially expressed after near-lethal heat stress treatments, and only modest shifts were observed. Analyses of upstream regulatory regions revealed loss or degradation of most HS (sigma32) promoters. Genomic rearrangements downstream of an intact HS promoter yielded upregulation of a functionally unrelated and an inactivated gene. Reanalyses of comparable experimental array data for E. coli and Bacillus subtilis revealed that genome-wide differential expression was significantly lower in B. aphidicola. Our demonstration of a diminished stress response validates reports of temperature sensitivity in B. aphidicola and suggests that this reduced bacterial genome exhibits transcriptional inflexibility.
Wilcox
Jennifer L
JL
Department of Ecology and Evolutionary Biology, University of Arizona, BioSciences West Rm 310, Tucson 85721, USA. wilcoxjl@email.arizona.edu
Dunbar
Helen E
HE
Wolfinger
Russell D
RD
Moran
Nancy A
NA
D000595:Amino Acid Sequence; D000818:Animals; D001042:Aphids / Q000382:microbiology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D020635:Buchnera / Q000235:genetics* / Q000502:physiology; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D019143:Evolution, Molecular; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D015321:Gene Rearrangement; D016680:Genome, Bacterial; D018869:Heat-Shock Response; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D020543:Proteome; D013559:Symbiosis; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D020543:Proteome