[An observation of the effects of recombinant human transforming growth factor beta3 on fibroblast].
OBJECTIVE
To investigate the role of recombinant human transforming growth factor beta3 (rhTGFbeta3) on fibroblast and its possible mechanism.


METHODS
Normal skin fibroblast (NSFb) and hypertrophic scar fibroblast (HSFb) were cultured in vitro, and were processed by different concentrations of rhTGFbeta3. NSFb and HSFb in DMEM solution without rhTGFbeta3 were employed as control. The changes in the protein and mRNA expression of type I and III collagen in NSFb and HSFb were observed.


RESULTS
(1) The expression of type I and III procollagen in NSFb was evidently different from that of HSFb (2) The synthesis of type I and III procollagen in all test groups was increased obviously after rhTGFbeta3 process (P < 0.001) while the ratio of type I to III procollagen was decreased when compared with that in control group. (3) The effects of rhTGFbeta3 on the biological behavior exhibited an obvious dose- effects relationship. The contents of type I to III procollagen in HSFb were higher than those in NSFb when the dose of rhTGFbeta3 was same.


CONCLUSIONS
rhTGFbeta3 could effectively promote the synthesis of type I and III procollagen, especially type III procollagen in fibroblasts. This might be beneficial to the accelerate of wound healing and to inhibit or prevent scar formation.
Lu
Luo
L
Department of Burns, Changhai Hospital, The Second Military Medical University, Shanghai 200433. P.R. China.
Chen
Yu-Lin
YL
Zhang
Qing-Guo
QG
D017439:Cicatrix, Hypertrophic / Q000378:metabolism*; D024042:Collagen Type I / Q000096:biosynthesis*; D024061:Collagen Type III / Q000096:biosynthesis*; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D011994:Recombinant Proteins / Q000494:pharmacology; D053782:Transforming Growth Factor beta3 / Q000494:pharmacology*
D024042:Collagen Type I; D024061:Collagen Type III; D011994:Recombinant Proteins; D053782:Transforming Growth Factor beta3