title: Epitope-blocking enzyme-linked immunosorbent assays for detection of west nile virus antibodies in domestic mammals.
abstract: We evaluated the ability of epitope-blocking enzyme-linked immunosorbent assays (ELISAs) to detect West Nile virus (WNV) antibodies in domestic mammals. Sera were collected from experimentally infected horses, cats, and pigs at regular intervals and screened in ELISAs and plaque reduction neutralization tests. The diagnostic efficacies of these techniques were similar.
authors.affiliation: Arthropod-Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA.
authors.forename: Bradley J
authors.initials: BJ
authors.lastname: Blitvich
authors.forename: Richard A
authors.initials: RA
authors.lastname: Bowen
authors.forename: Nicole L
authors.initials: NL
authors.lastname: Marlenee
authors.forename: Roy A
authors.initials: RA
authors.lastname: Hall
authors.forename: Michel L
authors.initials: ML
authors.lastname: Bunning
authors.forename: Barry J
authors.initials: BJ
authors.lastname: Beaty
mesh_terms: D000818:Animals; D000829:Animals, Domestic / Q000821:virology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000097:blood*; D002371:Cat Diseases / Q000821:virology; D002415:Cats; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes / Q000276:immunology; D006734:Horse Diseases / Q000821:virology; D006736:Horses; D009500:Neutralization Tests; D013552:Swine; D013553:Swine Diseases / Q000821:virology; D010948:Viral Plaque Assay; D014901:West Nile Fever / Q000175:diagnosis / Q000662:veterinary*; D014902:West Nile virus / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000939:Epitopes