title: Screening and selection of hypoallergenic buckwheat species.
abstract: Both common buckwheat (Fagopyrum esculentum) flour and meal cause an allergy in sensitive patients, and if unnoticed, it can be fatal. It has become a potential occupational hazard for some mill workers. The development of hypoallergenic buckwheat would be more efficient if natural mutants for allergenic protein are detected. A screening and selection method was developed using SDS-PAGE coupled with PCR techniques. SDS-PAGE analysis of 14 different species of buckwheat revealed that F. lineare and F. urophyllum lack the 22-kDa major allergenic protein. PCR-based screening with specific primers for sequences encoding the allergenic protein was also effective in distinguishing the allergen-deficient species.
authors.affiliation: Kiyomoto Bio Co. Ltd., 2-28-4 Sakura, Gakuen Kibanadai, Miyazaki 889-2154, Japan. nairarunc@yahoo.com
authors.forename: Arun
authors.initials: A
authors.lastname: Nair
authors.forename: Taiji
authors.initials: T
authors.lastname: Adachi
mesh_terms: D000485:Allergens / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D019612:Fagopyrum / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D009154:Mutation; D010940:Plant Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment
chemical_list: D000485:Allergens; D010940:Plant Proteins