Effects of gentamicin on the recovery of renal function after unilateral hydronephrosis.
BACKGROUND
Urinary tract infection is a complication of hydronephrosis and antibiotics such as gentamicin are indicated for treatment. However, gentamicin can cause drug-induced nephropathy in dehydrated patients. We used a rat kidney model to investigate the effects of gentamicin administration on functional recovery from unilateral hydronephrosis.


METHODS
Gentamicin was intraperitoneally injected twice for 48 hours following the release of a unilateral ureteral obstruction. The function of both kidneys was separately quantified by Technetium-99mDMSA renoscintigraphy. We examined morphological changes in renal tubular cells by electron microscopy and by in situ DNA 3'-end labeling.


RESULTS
Renal function in the contralateral, but not the obstructed, kidney was significantly damaged by gentamicin administration under our conditions and electron microscopy confirmed the presence of myeloid bodies in renal tubular cells. In situ DNA 3'-end labeling revealed characteristic damage to the renal tubules.


CONCLUSIONS
These results suggest that damage to each kidney should be considered individually after gentamicin administration during recovery from hydronephrosis.
Seki
Nobumitsu
N
Department of Urology, Ehime University School of Medicine, Shitsukawa, Shigenobu, Onsen-gun, Ehime 791-0295, Japan.
Tanji
Nozomu
N
Kubo
Shinji
S
Miyauchi
Yuuki
Y
Yokoyama
Masayoshi
M
D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002452:Cell Count; D004195:Disease Models, Animal; D005839:Gentamicins / Q000008:administration & dosage / Q000494:pharmacology*; D006869:Hydronephrosis / Q000209:etiology / Q000473:pathology / Q000503:physiopathology*; D020287:In Situ Nick-End Labeling; D007274:Injections, Intraperitoneal; D007684:Kidney Tubules / Q000187:drug effects / Q000648:ultrastructure; D008026:Ligation; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function / Q000187:drug effects*; D019783:Technetium Tc 99m Dimercaptosuccinic Acid; D014517:Ureteral Obstruction / Q000150:complications / Q000473:pathology / Q000503:physiopathology
D000900:Anti-Bacterial Agents; D005839:Gentamicins; D019783:Technetium Tc 99m Dimercaptosuccinic Acid