title: Optimisation of headspace solid-phase microextraction for the analysis of volatile phenols in wine.
abstract: Headspace solid-phase microextraction has been applied to the analysis of volatile phenols in wine. Silica fibre coated with Carbowax-divinylbenzene was found to be more efficient at extracting these compounds than other fibres such as those coated with polydimethylsiloxane, polyacrylate, carboxen-polydimethylsiloxane, and polydimethylsiloxane-divinylbenzene. Different parameters such as extraction time, temperature of the sample during the extraction, ionic strength and sample volume were optimised using a two-level factorial design expanded further to a central composite design, in order to evaluate several possibly influential and/or interacting factors. The headspace (HS)-SPME procedure developed shows adequate detection and quantitation limits, and linear ranges for correctly analysing these compounds in wine. The recoveries obtained were close to 100%, with repeatability values lower than 16%. The method was applied to a variety of white and red wines.
authors.affiliation: Analytical Chemistry Department, Faculty of Sciences, University of Cádiz, PO Box 40, E-11510 Pol. Rio San Pedro, Puerto Real, Cádiz, Spain. remedios.castro@uca.es
authors.forename: Remedios
authors.initials: R
authors.lastname: Castro Mejías
authors.forename: Ramón
authors.initials: R
authors.lastname: Natera Marín
authors.forename: M de Valme
authors.initials: Mde V
authors.lastname: García Moreno
authors.forename: Carmelo
authors.initials: C
authors.lastname: García Barroso
mesh_terms: D002849:Chromatography, Gas; D010636:Phenols / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014835:Volatilization; D014920:Wine / Q000032:analysis*
chemical_list: D010636:Phenols