title: Localization of brain-type fatty acid-binding protein in Kupffer cells of mice and its transient decrease in response to lipopolysaccharide.
abstract: Brain-type fatty acid-binding protein (B-FABP) was localized in Kupffer cells of liver of postnatal day 10 (P10) and older mice in immunolight and electron microscopy as well as by in situ hybridization histochemistry. The immunoreaction products were localized in the cytoplasmic matrix but not within the nucleus. After peritoneal injection of lipopolysaccharide (LPS), the immunoreaction for B-FABP decreased markedly in Kupffer cells at 1 h postinjection and thereafter gradually recovered to the preinjection level by 24 h postinjection, although no decrease in the mRNA expression was detected in Northern blotting throughout the course after the injection. The specific localization of B-FABP, but not the other FABPs, in Kupffer cells, and its rapid decrease after LPS injection suggest the intimate involvement of B-FABP in Kupffer cells in the inflammatory reaction, probably through mediation of n-3 polyunsaturated fatty acids, which are strong binders of B-FABP.
authors.affiliation: Division of Histology, Department of Cell Biology, Graduate School of Medical Science, Tohoku University, 980-8575 Sendai, Japan.
authors.forename: Soha Abdelkawi
authors.initials: SA
authors.lastname: Abdelwahab
authors.forename: Yuji
authors.initials: Y
authors.lastname: Owada
authors.forename: Noriko
authors.initials: N
authors.lastname: Kitanaka
authors.forename: Hiroo
authors.initials: H
authors.lastname: Iwasa
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sakagami
authors.forename: Hisatake
authors.initials: H
authors.lastname: Kondo
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015152:Blotting, Northern; D015153:Blotting, Western; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000276:immunology; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D017403:In Situ Hybridization; D007274:Injections, Intraperitoneal; D007728:Kupffer Cells / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000633:toxicity; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016253:Microscopy, Immunoelectron; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; C082667:Fabp5 protein, mouse; C086936:Fabp7 protein, mouse; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D008070:Lipopolysaccharides; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger