title: Phenotypic modulation of smooth muscle cells and vascular remodeling in intraparenchymal small cerebral arteries after canine experimental subarachnoid hemorrhage.
abstract: Cerebral microcirculatory changes are an important factor regulating cerebral blood flow. The aim of this study was to investigate the possibility of phenotypic modulation of smooth muscle cell (SMC) and vascular remodeling of intraparenchymal small cerebral arteries after subarachnoid hemorrhage (SAH). Seven to 14 days after canine experimental SAH, in intraparenchymal perforating arteries, the amount of beta-actin mRNA evaluated by Northern blot analysis increased, the structural change of the 3' untranslated region of beta-actin mRNA detected by polymerase chain reaction analysis was enhanced, and immunohistochemistry showed marked induction of the embryonal isoform of myosine heavy chain accompanied by decreased expression of smooth muscle myosin heavy chain (SM2). Histological morphometric analysis showed an increase in the area of the arterial wall without changes in the number of nuclei of SMC. This is the first report suggesting that vascular remodeling accompanied by phenotypic modulation occurs in intraparenchymal small arteries. These changes may affect cerebral blood flow after SAH by inducing increased cerebrovascular resistance.
authors.affiliation: Department of Neurosurgery, Hirosaki University School of Medicine, 5 Zaifu-cho, 036-8216, Hirosaki, Japan. ohkuma@cc.hirosaki-u.ac.jp
authors.forename: Hiroki
authors.initials: H
authors.lastname: Ohkuma
authors.forename: Shigeharu
authors.initials: S
authors.lastname: Suzuki
authors.forename: Kazumi
authors.initials: K
authors.lastname: Ogane
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D015152:Blotting, Northern; D002533:Cerebral Angiography; D002536:Cerebral Arteries / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology*; D004285:Dogs; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D018995:Myosin Heavy Chains / Q000378:metabolism; D010641:Phenotype; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013345:Subarachnoid Hemorrhage / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology*
chemical_list: D000199:Actins; D012333:RNA, Messenger; D018995:Myosin Heavy Chains