Chemical and biological interactions during nitrate and goethite reduction by Shewanella putrefaciens 200.
Although previous research has demonstrated that NO(3)(-) inhibits microbial Fe(III) reduction in laboratory cultures and natural sediments, the mechanisms of this inhibition have not been fully studied in an environmentally relevant medium that utilizes solid-phase, iron oxide minerals as a Fe(III) source. To study the dynamics of Fe and NO(3)(-) biogeochemistry when ferric (hydr)oxides are used as the Fe(III) source, Shewanella putrefaciens 200 was incubated under anoxic conditions in a low-ionic-strength, artificial groundwater medium with various amounts of NO(3)(-) and synthetic, high-surface-area goethite. Results showed that the presence of NO(3)(-) inhibited microbial goethite reduction more severely than it inhibited microbial reduction of the aqueous or microcrystalline sources of Fe(III) used in other studies. More interestingly, the presence of goethite also resulted in a twofold decrease in the rate of NO(3)(-) reduction, a 10-fold decrease in the rate of NO(2)(-) reduction, and a 20-fold increase in the amounts of N(2)O produced. Nitrogen stable isotope experiments that utilized delta(15)N values of N(2)O to distinguish between chemical and biological reduction of NO(2)(-) revealed that the N(2)O produced during NO(2)(-) or NO(3)(-) reduction in the presence of goethite was primarily of abiotic origin. These results indicate that concomitant microbial Fe(III) and NO(3)(-) reduction produces NO(2)(-) and Fe(II), which then abiotically react to reduce NO(2)(-) to N(2)O with the subsequent oxidation of Fe(II) to Fe(III).
Cooper
D Craig
DC
Environmental Science Research Center, School of Public and Environmental Affairs, Indiana University, Bloomington, Indiana 47405-1405, USA. coopdc@inel.gov
Picardal
Flynn W
FW
Schimmelmann
Arndt
A
Coby
Aaron J
AJ
D003470:Culture Media; D005290:Ferric Compounds / Q000378:metabolism; D005618:Fresh Water; D058085:Iron Compounds / Q000737:chemistry* / Q000378:metabolism*; D008903:Minerals; D009566:Nitrates / Q000737:chemistry* / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism; D009587:Nitrogen Isotopes / Q000378:metabolism; D009609:Nitrous Oxide / Q000378:metabolism; D010084:Oxidation-Reduction; D020614:Shewanella putrefaciens / Q000378:metabolism*
D003470:Culture Media; D005290:Ferric Compounds; D058085:Iron Compounds; D008903:Minerals; D009566:Nitrates; D009573:Nitrites; D009587:Nitrogen Isotopes; C094886:goethite; D009609:Nitrous Oxide