title: Characterization of urease-positive thermophilic Campylobacter subspecies by multilocus enzyme electrophoresis typing.
abstract: Thirty-one urease-positive thermophilic Campylobacter (UPTC) isolates, including three reference strains (NCTC12892, NCTC12895 and NCTC12896), and three Campylobacter lari isolates, which were isolated from several countries and sources, were compared genotypically by using multilocus enzyme electrophoresis (MLEE). We examined allelic variation around seven enzyme loci, including the adenylate kinase, alkaline phosphatase, catalase, fumarase, malic enzyme, malate dehydrogenase, and L-phenylalanyl-L-leucine peptidase loci. MLEE typing revealed the presence of 23 different electrophoretic types (ETs) among the 31 UPTC isolates, and 14 isolates shared six electrophoretic profiles. Three different ETs were identified for the three C. lari isolates examined, and no ETs were shared by UPTC and C. lari isolates. Quantitative analyses were subsequently performed by using allelic variation data, and the results demonstrated that the mean genetic diversity was 0.655. In conclusion, MLEE demonstrated that the UPTC isolates examined are genetically hypervariable and form a cluster separate from the C. lari cluster.
authors.affiliation: Laboratory of Molecular Biology, School of Environmental Health Sciences, Azabu University, Sagamihara 229, Japan.
authors.forename: Motoo
authors.initials: M
authors.lastname: Matsuda
authors.forename: Aki
authors.initials: A
authors.lastname: Kaneko
authors.forename: Timothy
authors.initials: T
authors.lastname: Stanley
authors.forename: B Cherie
authors.initials: BC
authors.lastname: Millar
authors.forename: M
authors.initials: M
authors.lastname: Miyajima
authors.forename: Philip G
authors.initials: PG
authors.lastname: Murphy
authors.forename: John E
authors.initials: JE
authors.lastname: Moore
mesh_terms: D015373:Bacterial Typing Techniques; D002167:Campylobacter / Q000145:classification* / Q000201:enzymology / Q000235:genetics*; D004586:Electrophoresis / Q000379:methods; D004798:Enzymes / Q000032:analysis; D014644:Genetic Variation; D005838:Genotype; D006358:Hot Temperature; D013045:Species Specificity; D014510:Urease / Q000378:metabolism*
chemical_list: D004798:Enzymes; D014510:Urease