[Virus resistance in transgenic watermelon plants containing a WMV-2 coat protein gene].
Virus disease is a major cause that affects the quality and output of watermelon which is an important fruit in summer. So it is really urgent to develop disease resistance plants. But it takes a long time to breed such plants in conventional ways, and it is very difficult to get ideal result. With the development of plant genetic engineering, new ways have been found to breed plants with disease resistance. By using plant transgenic technique, much progress was been made in plant improvement. There are many successful cases of transgenic plants against corresponding virus disease through transferring coat protein gene. This paper reports the results of inheritance, segregation, expression of WMV-2 coat protein gene in inbred transgenic watermelon and its resistance to virus. Through PCR analysis of inbred plants, we found WMV-2 coat protein gene in the genome of progeny R1 separated with 3:1. After successive selection and identification of 4 generations, 8 transgenic pure lines with almost the same agronomic traits were obtained from 3 independent transformants of T7, T11 and T32. The result of Western blotting shows all 3 different transgenic lines of R4T7-1, R4T11-3 and R4T32-7 can produce coat protein. Disease resistance experiment on transgenic plants with WMV-2 shows that, compared with the control groups, transgenic plants can delay the disease infection and reduce the incidence and the symptoms of virus disease. And the transgenic line R4T32-7 expressed high resistance to infection by WMV-2, which lays a foundation for breeding of disease resistant varieties through plant transgenic technique.
Wang
Hui-Zhong
HZ
National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China. whzsyj@mail.hz.zj.cn
Zhao
Pei-Jie
PJ
Xu
Ji-Chen
JC
Zhao
Huai
H
Zhang
Hong-Sheng
HS
D015153:Blotting, Western; D036022:Capsid Proteins / Q000235:genetics* / Q000378:metabolism; D029773:Citrullus / Q000235:genetics* / Q000821:virology; D007113:Immunity, Innate / Q000235:genetics; D009029:Mosaic Viruses / Q000235:genetics / Q000254:growth & development*; D010935:Plant Diseases / Q000235:genetics / Q000821:virology; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D013997:Time Factors
D036022:Capsid Proteins