Liquid chromatographic determination of hydroxyproline in tissue samples.
We describe a reversed-phase assay of hydroxyproline in rat lung tissue using sarcosine for the internal standard and pre-injection reaction with both o-phthalaldehyde (OPA) and 9-fluorenylmethylchloroformate (FMOC). Intra-assay variability in the concentration range of 25-500 microM hydroxyproline was less than 1%. Normal rat (left) lung was found to have a hydroxyproline content of 1.08+/-0.18 mg/lung. This ability to measure minute amounts of hydroxyproline is being applied to the measure of collagen and pathological fibrosis.
Hutson
Paul R
PR
UW School of Pharmacy, 777 Highland Avenue, Madison, WI 53705-2222 USA. prhutson@pharmacy.wisc.edu
Crawford
Mark E
ME
Sorkness
Ronald L
RL
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006909:Hydroxyproline / Q000032:analysis*; D008168:Lung / Q000737:chemistry; D051381:Rats; D012015:Reference Standards; D013050:Spectrometry, Fluorescence; D009764:o-Phthalaldehyde / Q000737:chemistry
D009764:o-Phthalaldehyde; D006909:Hydroxyproline