A comparison of T-, B- and NK-cell reconstitution following conventional or nonmyeloablative conditioning and transplantation with bone marrow or peripheral blood stem cells from human leucocyte antigen identical sibling donors.
This retrospective study compares the reconstitution of T, B and NK cells in three groups of patients transplanted for haematological malignancies with grafts from their HLA-identical sibling donors. In all, 15 patients received PBSC after a nonmyeloablative conditioning regimen consisting of fludarabine and 200 cGy TBI, 13 patients received PBSC after myeloablative conditioning and 37 patients received BM after myeloablative conditioning. In the nonmyeloablative group, the NK cells normalised after 1 month, the CD8+ T cells normalised after 3 months, the CD4+ T cells reached near normal values after 9 months and the B cell values were reduced until 12 months after transplant. In the two myeloablative groups, recipients of PBSC had a significantly higher number of CD4+ T cells after 4 months (P=0.004) and after 12 months (P=0.001), than recipients of BM. We found no differences in the T cell reconstitution between the two PBSC groups. This was of interest as the recipients of nonmyeloablative conditioning were older (P<0.001) and had a higher occurrence of chronic GVHD (P<0.05) than the recipients of myeloablative conditioning. In contrast, the recipients of nonmyeloablative conditioning had a delayed B cell recovery when compared to the patients who received myeloablative conditioning (P=0.04).
Petersen
S L
SL
The Lymphocyte Research Laboratory, Rigshospitalet, Copenhagen, Denmark.
Ryder
L P
LP
Björk
P
P
Madsen
H O
HO
Heilmann
C
C
Jacobsen
N
N
Sengeløv
H
H
Vindeløv
L L
LL
D000293:Adolescent; D000328:Adult; D001402:B-Lymphocytes / Q000502:physiology; D016026:Bone Marrow Transplantation / Q000379:methods*; D005260:Female; D006085:Graft Survival; D006086:Graft vs Host Disease / Q000139:chemically induced / Q000209:etiology; D019337:Hematologic Neoplasms / Q000628:therapy; D006650:Histocompatibility Testing; D006801:Humans; D007694:Killer Cells, Natural / Q000502:physiology; D038041:Lymphopoiesis; D008297:Male; D008875:Middle Aged; D036102:Peripheral Blood Stem Cell Transplantation / Q000379:methods*; D012044:Regression Analysis; D012189:Retrospective Studies; D035781:Siblings; D013601:T-Lymphocytes / Q000502:physiology; D019172:Transplantation Conditioning / Q000379:methods*; D014184:Transplantation, Homologous; D014185:Transplantation, Isogeneic