Structure of the O-specific polysaccharide from the lipopolysaccharide of Citrobacter gillenii O11, strain PCM 1540.
The O-specific polysaccharide of the lipopolysaccharide of Citrobacter gillenii PCM 1540 (serogroup O11) consists of D-Glc, D-Man, D-GalNAc, D-GlcNAc, 2-acetamido-2,6-dideoxy-D-galactose (D-FucNAc) and O-acetyl groups in the ratios 2:1:1:1:1:1. On the basis of sugar and methylation analyses and Smith-degradation along with 1D and 2D 1H and 13C NMR spectroscopy, the following structure of the branched hexasaccharide repeating unit was established: [structure: see text]. Citrobacter werkmanii PCM 1541 belonging to the same serogroup O11 was found to have an R-form lipopolysaccharide devoid of the O-specific polysaccharide.
Katzenellenbogen
Ewa
E
Polish Academy of Sciences, L. Hirszfeld Institute of Immunology and Experimental Therapy, Weigla 12, 53-114, Wrocław, Poland. katzenel@iitd.pan.wroc.pl
Kocharova
Nina A
NA
Zatonsky
George V
GV
Bogulska
Maria
M
Rybka
Jacek
J
Gamian
Andrzej
A
Shashkov
Alexander S
AS
Knirel
Yuriy A
YA
D000085:Acetates / Q000032:analysis; D000116:Acetylgalactosamine / Q000032:analysis; D000117:Acetylglucosamine / Q000032:analysis; D002240:Carbohydrate Sequence; D002954:Citrobacter / Q000737:chemistry* / Q000145:classification; D005643:Fucose / Q000031:analogs & derivatives* / Q000032:analysis; D005947:Glucose / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D008358:Mannose / Q000032:analysis; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D025942:Oligosaccharides, Branched-Chain / Q000737:chemistry*; D012703:Serotyping
D000085:Acetates; D019081:O Antigens; D025942:Oligosaccharides, Branched-Chain; D005643:Fucose; C059377:N-acetylfucosamine; D005947:Glucose; D000116:Acetylgalactosamine; D008358:Mannose; D000117:Acetylglucosamine