The unusual effect of pKM101 on the mutagenicity of acetaldehyde oxime in Salmonella typhimurium.
Acetaldehyde oxime was found to induce more revertants in Salmonella typhimurium strain TA1535 than in TA100 in the absence of S9 metabolic activation. TA100 was originally constructed from TA1535 by the addition of the plasmid pKM101, carrying mucAB which generally enhances sensitivity to the mutagenic effects of chemicals. The role of pKM101 in lowering the sensitivity to acetaldehyde oxime was explored by: (1) increasing the incubation time of the selective agar plates from 2 to 3 days; (2) using a new strain, isogenic to TA100, constructed by introducing pKM101 into the TA1535 isolate used in these experiments; (3) by testing a strain constructed by inserting into TA1535 a plasmid carrying mucAB but otherwise unrelated to pKM101. Each of these alterations increased the number of revertants per plate in the presence of acetaldehyde oxime, indicating that the apparent nonmutagenicity of this chemical in TA100 is due to multiple factors.
Prival
Michael J
MJ
Division of In Vitro and Biochemical Toxicology (HFS-25), U.S. Food and Drug Administration, 8301 Muirkirk Road, Laurel, MD 20708, USA. mprival@aol.com
D004305:Dose-Response Relationship, Drug; D018274:Electroporation; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics; D009153:Mutagens / Q000633:toxicity*; D010091:Oximes / Q000633:toxicity*; D010957:Plasmids / Q000235:genetics*; D017354:Point Mutation / Q000187:drug effects; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics*; D013045:Species Specificity
D029968:Escherichia coli Proteins; D009153:Mutagens; D010091:Oximes; C068606:mucAB protein, E coli; C023728:acetaldehyde oxime