Identification of NDRG1 as an early inducible gene during in vitro maturation of cultured mast cells.
Coculture of mouse bone marrow-derived mast cells (BMMC) with fibroblasts in the presence of stem cell factor (SCF) facilitates morphological and functional maturation toward a connective tissue mast cell (CTMC)-like phenotype. By means of cDNA subtraction, we identified several inducible genes during this mast cell maturation process. Of approximately 100 sequenced clones induced, nearly 50% were chromosome 14-associated serine proteases. Approximately 14% encoded NDRG1, a 43-kDa cytosolic protein that has been implicated in cell differentiation. NDRG1 was distributed in the cytosol of cultured mast cells and CTMC in rat skin. Overexpression of NDRG1 in RBL-2H3 cells resulted in enhanced degranulation in response to various stimuli. Thus, NDRG1 may be a mast cell maturation-associated inducible protein that allows the cells to be susceptible to extracellular stimuli leading to degranulation. Additionally, several unique maturation-associated inducible genes were identified, molecular and functional characterization of which will provide new insights into mast cell biology.
Taketomi
Yoshitaka
Y
Department of Health Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, 142-8555, Tokyo, Japan.
Sugiki
Toshihiko
T
Saito
Takanori
T
Ishii
Shin ichi
Si
Hisada
Masato
M
Suzuki-Nishimura
Tamiko
T
Uchida
Masaatsu K
MK
Moon
Tae Chul
TC
Chang
Hyeun Wook
HW
Natori
Yasuhiro
Y
Miyazawa
Shinobu
S
Kikuchi-Yanoshita
Rei
R
Murakami
Makoto
M
Kudo
Ichiro
I
D000595:Amino Acid Sequence; D000818:Animals; D018797:Cell Cycle Proteins / Q000096:biosynthesis* / Q000235:genetics*; D002460:Cell Line; D002478:Cells, Cultured; D018920:Coculture Techniques; D003600:Cytosol / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000378:metabolism; D007150:Immunohistochemistry; D007249:Inflammation; D007313:Insecta; D047908:Intracellular Signaling Peptides and Proteins; D008024:Ligands; D008407:Mast Cells / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D018613:Microscopy, Confocal; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D016133:Polymerase Chain Reaction; D017434:Protein Structure, Tertiary; D051381:Rats; D014162:Transfection; D000099259:N-myc Downstream-Regulated Gene 1 Protein
D018797:Cell Cycle Proteins; D018076:DNA, Complementary; D047908:Intracellular Signaling Peptides and Proteins; D008024:Ligands; D000099259:N-myc Downstream-Regulated Gene 1 Protein