title: The impact of intestinal ischaemia-reperfusion on caerulein-induced oedematous experimental pancreatitis.
abstract: BACKGROUND
  Intestinal ischaemia is a feature of severe acute pancreatitis. It is not known whether intestinal ischaemia and reperfusion contributes to the progression from mild to severe pancreatitis.
  
  
  OBJECTIVE
  The aim of this study was to examine the impact of intestinal ischaemia-reperfusion on caerulein-induced oedematous experimental pancreatitis.
  
  
  METHODS
  Male Wistar rats (n = 48) were randomised to 6 experimental groups: controls (CO), saline infusion (S), saline infusion and intestinal ischaemia-reperfusion (SIR), caerulein infusion (C), caerulein and sham operation (CS), and caerulein infusion with intestinal ischaemia reperfusion (CIR). Caerulein was infused over 6 h to induce mild oedematous pancreatitis. Clamping the superior mesenteric artery for 10 min induced mild intestinal ischaemia. The reperfusion time was 24 h. The primary end point was histology of the pancreas at 24 h.
  
  
  RESULTS
  There was no significant difference in histologic severity of pancreatitis at 24 h (Kruskal-Wallis, p = 0.37).
  
  
  CONCLUSIONS
  The severity of acute oedematous pancreatitis was not increased by 10 min of intestinal ischaemia followed by 24 h of reperfusion.
authors.affiliation: Department of Surgery, Faculty of Medical and Health Sciences, University of Auckland, Auckland, New Zealand. g.farrant@auckland.ac.nz
authors.forename: G J
authors.initials: GJ
authors.lastname: Farrant
authors.forename: F M
authors.initials: FM
authors.lastname: Abu-Zidan
authors.forename: X
authors.initials: X
authors.lastname: Liu
authors.forename: B
authors.initials: B
authors.lastname: Delahunt
authors.forename: L J
authors.initials: LJ
authors.lastname: Zwi
authors.forename: J A
authors.initials: JA
authors.lastname: Windsor
mesh_terms: D000208:Acute Disease; D000818:Animals; D002108:Ceruletide; D004487:Edema / Q000139:chemically induced / Q000150:complications* / Q000473:pathology*; D007422:Intestines / Q000473:pathology*; D008297:Male; D008638:Mesenteric Arteries; D009842:Oligopeptides / Q000378:metabolism; D010179:Pancreas / Q000473:pathology; D010195:Pancreatitis / Q000139:chemically induced / Q000150:complications* / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000150:complications* / Q000473:pathology*; D013525:Surgical Instruments
chemical_list: D009842:Oligopeptides; C080396:trypsinogen activation peptide; D002108:Ceruletide