title: Constriction and Dnm1p recruitment are distinct processes in mitochondrial fission.
abstract: Mitochondria undergo cycles of fusion and fission crucial for organelle homeostasis. Fission is regulated partially by recruitment of the large GTPase Dnm1p to the outer mitochondrial membrane. Using three-dimensional time-lapse fluorescence imaging of Saccharomyces cerevisiae cells, we found that Dnm1p-EGFP appears and disappears at "hot spots" along mitochondrial tubes. It forms patches that convert rapidly into different shapes regardless of whether mitochondrial fission ensues or not. Moreover, the thickness of the mitochondrial matrix displays frequent temporal fluctuations apparently unrelated to fission or to recruitment of Dnm1p-EGFP. These results suggest that mitochondrial fission requires coordination of at least two distinct processes.
authors.affiliation: Department of Cell Biology and The Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: Aster
authors.initials: A
authors.lastname: Legesse-Miller
authors.forename: Ramiro H
authors.initials: RH
authors.lastname: Massol
authors.forename: Tom
authors.initials: T
authors.lastname: Kirchhausen
mesh_terms: D020558:GTP Phosphohydrolases / Q000502:physiology*; D017930:Genes, Reporter; D008928:Mitochondria / Q000502:physiology*; D024101:Mitochondrial Proteins; D012441:Saccharomyces cerevisiae / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins / Q000502:physiology*
chemical_list: D024101:Mitochondrial Proteins; D029701:Saccharomyces cerevisiae Proteins; D020558:GTP Phosphohydrolases; C094774:DNM1 protein, S cerevisiae