Physical interaction between RRS1-R, a protein conferring resistance to bacterial wilt, and PopP2, a type III effector targeted to the plant nucleus.
RRS1-R confers broad-spectrum resistance to several strains of the causal agent of bacterial wilt, Ralstonia solanacearum. Although genetically defined as recessive, this R gene encodes a protein whose structure combines the TIR-NBS-LRR domains found in several R proteins and a WRKY motif characteristic of some plant transcriptional factors and behaves as a dominant gene in transgenic susceptible plants. Here we show that PopP2, a R. solanacearum type III effector, which belongs to the YopJ/AvrRxv protein family, is the avirulence protein recognized by RRS1-R. Furthermore, an interaction between PopP2 and both RRS1-R and RRS1-S, present in the resistant Nd-1 and susceptible Col-5 Arabidopsis thaliana ecotypes, respectively, was detected by using the yeast split-ubiquitin two-hybrid system. This interaction, which required the full-length R protein, was not observed between the RRS1 proteins and PopP1, another member of the YopJ/AvrRxv family present in strain GMI1000 and that confers avirulence in Petunia. We further demonstrate that both the Avr protein and the RRS1 proteins colocalize in the nucleus and that the nuclear localization of the RRS1 proteins are dependent on the presence of PopP2.
Deslandes
Laurent
L
Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Carl-von-Linne-Weg 10, D-50829 Cologne, Germany. Ldesland@mpiz-koeln.mpg.de
Olivier
Jocelyne
J
Peeters
Nemo
N
Feng
Dong Xin
DX
Khounlotham
Manirath
M
Boucher
Christian
C
Somssich
Imre
I
Genin
Stephane
S
Marco
Yves
Y
D020816:Amino Acid Motifs; D017360:Arabidopsis / Q000235:genetics; D001426:Bacterial Proteins / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D020022:Genetic Predisposition to Disease; D016946:Gram-Negative Aerobic Rods and Cocci / Q000378:metabolism; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000378:metabolism; D018613:Microscopy, Confocal; D008954:Models, Biological; D009687:Nuclear Proteins / Q000737:chemistry / Q000378:metabolism*; D010935:Plant Diseases / Q000235:genetics; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D013997:Time Factors; D020798:Two-Hybrid System Techniques; D025801:Ubiquitin / Q000378:metabolism; D000097573:Red Fluorescent Protein
D001426:Bacterial Proteins; D004247:DNA; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D009687:Nuclear Proteins; D025801:Ubiquitin; D000097573:Red Fluorescent Protein; C109227:YopP protein, Yersinia