Identification of heterofermentative lactobacilli isolated from pig faeces by numerical analysis of total soluble cell protein patterns and RAPD-PCR.
OBJECTIVE
The study deals with a number of heterofermentative Lactobacillus strains isolated from pig faeces and their identification.


RESULTS
SDS-PAGE of total soluble cell proteins and RAPD-PCR profiles were used to identify the strains isolated from pig faeces. Protein profiles obtained with SDS-PAGE revealed that 15 strains clustered at r >or= 0.78 with Lactobacillus buchneri and nine strains at r >or= 0.77 with two reference strains of Lactobacillus reuteri. The identity of the strains was confirmed with RAPD-PCR.


CONCLUSIONS
Numerical analysis of protein profiles and RAPD-PCR proved valuable in the differentiation of Lactobacillus spp. isolated from pig faeces.


CONCLUSIONS
This is the first report on the association of Lact. buchneri with pig faeces.
Du Toit
M
M
Department of Microbiology, Stellenbosch University, Private Bag X1, Matieland ZA-7602 South Africa.
Dicks
L M T
LM
Holzapfel
W H
WH
D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis*; D001482:Base Composition; D005243:Feces / Q000382:microbiology*; D005285:Fermentation; D005960:Glucosides / Q000378:metabolism; D007778:Lactobacillus / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D010802:Phylogeny; D019105:Random Amplified Polymorphic DNA Technique; D034421:Sus scrofa / Q000382:microbiology*; D013696:Temperature
D001426:Bacterial Proteins; D005960:Glucosides