Genes involved in beta-oxidation, energy metabolism and glyoxylate cycle are induced by Candida albicans during macrophage infection.
The ability of intracellular pathogens to cause infection is related to their capacity to survive and grow inside macrophages or in other cell types. Candida albicans latent virulence is likely to be related to a similar mechanism of avoiding killing by specialized cells and to the resulting ability to grow in such hostile environments. Using a differential display reverse transcription polymerase chain reaction technique, we have identified seven genes induced in C. albicans during macrophage phagocytosis. Sequence analyses and database searches revealed that these cDNAs coded for proteins homologous to yeast metabolic proteins. Interestingly, four of them are putative peroxisomal proteins, and two are involved in environmental signal sensing and transduction. Among the seven genes induced by C. albicans, six represent new information that were not described in other infection models.
Prigneau
Odile
O
Laboratory of Molecular Fungal Pathogenesis, Institute of Genetics and Biophysics Buzzati-Traverso-CNR, 80125 Naples, Italy.
Porta
Amalia
A
Poudrier
Jacques A
JA
Colonna-Romano
Sergio
S
Noël
Thierry
T
Maresca
Bruno
B
D015152:Blotting, Northern; D002176:Candida albicans / Q000235:genetics* / Q000378:metabolism; D002177:Candidiasis / Q000378:metabolism; D003001:Cloning, Molecular; D004734:Energy Metabolism / Q000235:genetics* / Q000502:physiology; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal / Q000502:physiology*; D005800:Genes, Fungal / Q000235:genetics / Q000502:physiology; D006038:Glyoxylates / Q000378:metabolism*; D006801:Humans; D008264:Macrophages / Q000378:metabolism / Q000382:microbiology*; D010084:Oxidation-Reduction; D020675:Peroxisomes / Q000235:genetics / Q000378:metabolism; D010587:Phagocytosis / Q000502:physiology*; D016133:Polymerase Chain Reaction; D012331:RNA, Fungal / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA
D006038:Glyoxylates; D012331:RNA, Fungal