Hemodynamic improvement and removal of autoantibodies against beta1-adrenergic receptor by immunoadsorption therapy in dilated cardiomyopathy.
The removal of beta(1)-adrenergic receptor (beta(1)AR) autoantibodies by immunoadsorption (IA) has been proposed as a potential mechanism for the improvement of the left ventricular function in dilated cardiomyopathy (DCM). In the present study, the possible association between removal of the autoantibodies against the human beta(1)AR with the hemodynamic improvement induced by IA was investigated.IA was performed in 22 DCM patients (n=22; NYHA III-IV, EF<30%, stable medication). The beta(1)AR autoantibodies from column eluents (CE) were detected by enzyme-linked immunosorbent assay (ELISA) and BIAcore methods. CE of 32% (7/22) of the patients was found to be antibody-positive with ELISA or BIAcore. In addition, a bioassay system was also used for the detection of this autoantibody. Seventy-three percent (16/22) of the patients were found to be antibody-positive by this method. However, independent of the beta(1)AR antibody detection method, both antibody-positive and antibody-negative groups showed similar acute and prolonged hemodynamic improvements during IA therapy. Furthermore, antibody-positive and -negative groups received a comparable improvement of left ventricular ejection fraction. These results suggest that different mechanisms are involved in the hemodynamic improvement induced by IA. The beneficial hemodynamic effects induced by IA are not directly associated with the removal of beta(1)AR autoantibodies.
Mobini
Reza
R
The Cardiovascular Institute, Sahlgrenska University Hospital, Göteborg, Sweden. reza.mobini@wlab.gu.se
Staudt
Alexander
A
Felix
Stephan B
SB
Baumann
Gert
G
Wallukat
Gert
G
Deinum
Johanna
J
Svensson
Helen
H
Hjalmarson
Ake
A
Fu
Michael
M
D001323:Autoantibodies / Q000097:blood / Q000302:isolation & purification*; D002311:Cardiomyopathy, Dilated / Q000276:immunology / Q000503:physiopathology / Q000628:therapy*; D016022:Case-Control Studies; D006439:Hemodynamics; D006801:Humans; D007163:Immunosorbent Techniques; D007167:Immunotherapy; D008297:Male; D008875:Middle Aged; D018342:Receptors, Adrenergic, beta-1 / Q000276:immunology*; D016277:Ventricular Function, Left
D001323:Autoantibodies; D018342:Receptors, Adrenergic, beta-1