Chromosomal location of the genetic determinants for bacteriocins produced by Lactobacillus gasseri K7.
The production of similar or even identical bacteriocins by different lactic acid bacteria is not a rare event. To take advantage of this finding, genetic determinants of the Lactobacillus K7 bacteriocins were tested for putative homologies with previously described bacteriocins of the Lactobacillus acidophilus group through polymerase chain reaction (PCR). Among specific primer pairs of seven known bacteriocins, derived from their respective sequences, only acidocin LF221 A and B primers amplified fragments in chromosomal DNA of K7 strain that revealed strong similarity over small regions of LF221 bacteriocins. Treatment of Lactobacillus K7 with ethidium bromide and mitomycin C was ineffective in generating non-bacteriocinogenic derivatives and had no impact on plasmid loss either. Classification studies elucidated Lactobacillus K7 as a member of the Lactobacillus gasseri species.
Canzek Majhenic
Andreja
A
University of Ljubljana, Biotechnical Faculty, Groblje 3, SI-1230 Domzale, Slovenia.
Bogovic Matijasić
Bojana
B
Rogelj
Irena
I
D001430:Bacteriocins / Q000096:biosynthesis / Q000235:genetics*; D002874:Chromosome Mapping; D017931:DNA Primers; D004996:Ethidium / Q000494:pharmacology; D007778:Lactobacillus / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D016685:Mitomycin / Q000494:pharmacology; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction
D001430:Bacteriocins; D017931:DNA Primers; C093079:acdA protein, Lactobacillus; C096679:gassericin A; C420222:gassericin T; C090262:acidocin B protein, Lactobacillus; D016685:Mitomycin; D004996:Ethidium