title: Reactivity of new adhesion molecules on lymphocytes from patients with chronic graft versus host disease.
abstract: Reaction patterns of the 7th Human Leukocyte Differentiation Antigen Workshop blind panel adhesion molecules were studied on CD3/CD4, CD3/CD8, CD3/TCR gamma delta double positive T cells from peripheral blood of patients with chronic graft versus host disease (n = 8) and healthy controls (n = 4). Reactivity of 14 adhesion antibodies was tested by three-colour immunophenotyping. The mean proportion of CD3+ T cells (69 +/- 19%). CD3/CD8++ (31 +/- 13%) and CD3/TCR gamma delta++ (4 +/- 2%) T sub-populations of patients were comparable with the healthy controls. However, the mean percentage of CD3/CD4++ T cell subset in patients (14 +/- 12%) proved to be significantly decreased in comparison with the normal control value (34 +/- 16%) presumably due to secondary immunodeficiency. The workshop antibodies proved to be reactive with three T cell subsets expressing the examined antigens. Based on the results of the adhesion molecule workshop new CD categories have been introduced: CD156b as a transmembrane protein, CD167a as an epithelial tyrosin kinase receptor, CD168 as a receptor for hyaluronan mediated motility (RHAMM) and CD171 as a co-stimulatory adhesion molecule. There were significant differences in the expression of the CD167a and CD156b antigens on the CD3/CD4++ subset between the samples of patients compared with the controls characterizing the CD4+ T lymphocyte subpopulation in chronic graft versus host disease.
authors.affiliation: National Medical Centre, Institute of Haematology and Immunology, Research Group of Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: Nóra
authors.initials: N
authors.lastname: Regéczy
authors.forename: Luca
authors.initials: L
authors.lastname: Kormos
authors.forename: Csilla M
authors.initials: CM
authors.lastname: Szigetvári
authors.forename: Eva
authors.initials: E
authors.lastname: Torbágyi
authors.forename: Melinda
authors.initials: M
authors.lastname: Hajdu
authors.forename: L
authors.initials: L
authors.lastname: Gopcsa
authors.forename: Anikó
authors.initials: A
authors.lastname: Bányai
authors.forename: Katalin
authors.initials: K
authors.lastname: Pálóczi
mesh_terms: D051722:ADAM Proteins; D000328:Adult; D015703:Antigens, CD / Q000378:metabolism*; D017252:CD3 Complex / Q000378:metabolism; D015704:CD4 Antigens / Q000378:metabolism; D016827:CD8 Antigens / Q000378:metabolism; D002908:Chronic Disease; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005260:Female; D006086:Graft vs Host Disease / Q000276:immunology*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D016130:Immunophenotyping; D008297:Male; D008565:Membrane Proteins; D008666:Metalloendopeptidases / Q000378:metabolism; D008875:Middle Aged; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism*; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D014184:Transplantation, Homologous
chemical_list: D015703:Antigens, CD; D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens; D016326:Extracellular Matrix Proteins; D018960:Hyaluronan Receptors; D008565:Membrane Proteins; D016692:Receptors, Antigen, T-Cell, gamma-delta; C098946:hyaluronan-mediated motility receptor; D051722:ADAM Proteins; C068490:ADAM8 protein, human; D008666:Metalloendopeptidases