Evaluation of genotype MTBC assay for differentiation of clinical Mycobacterium tuberculosis complex isolates.
A commercially available DNA strip assay was evaluated for the ability to differentiate Mycobacterium tuberculosis complex species. M. bovis subsp. bovis, M. bovis subsp. caprae, M. bovis BCG, M. africanum subtype I, and M. microti were unequivocally identified. M. tuberculosis, M. canetti, and M. africanum subtype II showed a unique hybridization pattern.
Richter
Elvira
E
Forschungszentrum Borstel, National Reference Center for Mycobacteria, D-23845 Borstel, Germany. erichter@fz-borstel.de
Weizenegger
Michael
M
Rüsch-Gerdes
Sabine
S
Niemann
Stefan
S
D000818:Animals; D015373:Bacterial Typing Techniques; D002417:Cattle; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis; D005838:Genotype; D006801:Humans; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics; D009164:Mycobacterium Infections / Q000382:microbiology* / Q000662:veterinary; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics; D009693:Nucleic Acid Hybridization / Q000379:methods*; D011934:Reagent Strips
D004269:DNA, Bacterial; D011934:Reagent Strips; D027081:DNA Gyrase