Synthetic gene libraries: in search of the optimal diversity.
Directed evolution has proven to be an effective method for evolving proteins with desired properties. A key step is the creation of suitably diverse gene libraries. Two new methods for creating such libraries make sole use of synthesized oligonucleotides and allow researchers to tailor the diversity of a library with greater precision and create libraries with greater diversity than was previously possible. Such increased diversity appears to accelerate directed evolution.
Ostermeier
Marc
M
Dept of Chemical and Biomolecular Engineering, Johns Hopkins University, 3400 N. Charles St, Baltimore, MD 21218, USA. oster@jhu.edu
D003001:Cloning, Molecular / Q000379:methods; D019020:Directed Molecular Evolution / Q000379:methods*; D004815:Epidermal Growth Factor / Q000235:genetics; D015723:Gene Library; D005813:Genes, Synthetic; D005818:Genetic Engineering / Q000379:methods*; D014644:Genetic Variation; D019151:Peptide Library; D015202:Protein Engineering / Q000379:methods; D011506:Proteins / Q000235:genetics*; D011995:Recombination, Genetic; D020860:Subtilisin / Q000235:genetics
D019151:Peptide Library; D011506:Proteins; D004815:Epidermal Growth Factor; D020860:Subtilisin