title: Identification of selenoprotein P fragments as a cell-death inhibitory factor.
abstract: Megakaryoblastoma (Dami cells) cultured in a serum-free medium containing albumin, proliferated for three days but died on the fourth day. This cell death was not observed when human plasma was added, suggesting that human plasma contains a cell-death inhibitory factor. In order to identify this factor, we purified it from human plasma. N-terminal amino acid sequence analysis revealed that this factor is a mixture of C-terminal fragments of selenoprotein P, a major selenocysteine-containing protein in plasma. The specific activity (unit per pmol of selenium) of selenoprotein P fragments protein was 15-fold and 1900-fold higher than that of the full-length SeP and sodium selenite, respectively.
authors.affiliation: The Chemo-Sero-Therapeutic Research Institute, Kawabe, Kyokushi, Kikuchi-gun, Kumamoto 869-1298, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Hirashima
authors.forename: Takeshi
authors.initials: T
authors.lastname: Naruse
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Maeda
authors.forename: Chikateru
authors.initials: C
authors.lastname: Nozaki
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Saito
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Takahashi
mesh_terms: D000595:Amino Acid Sequence; D000975:Antioxidants / Q000494:pharmacology; D016923:Cell Death / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D016895:Culture Media, Serum-Free; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification* / Q000494:pharmacology; D010949:Plasma / Q000737:chemistry; D011506:Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D012643:Selenium / Q000494:pharmacology; D051149:Selenoprotein P; D051140:Selenoproteins; D014407:Tumor Cells, Cultured
chemical_list: D000975:Antioxidants; D016895:Culture Media, Serum-Free; D010446:Peptide Fragments; D011506:Proteins; D051149:Selenoprotein P; D051140:Selenoproteins; D012643:Selenium