title: Use of co-immobilized beta-amylase and pullulanase in reduction of saccharification time of starch and increase in maltose yield.
abstract: Beta-amylase and pullulanase were co-immobilized to poly(acrylamide-acrylic acid) resin [P(AAm-AAc)] using 1-ethyl-3-(3-dimethylaminopropyl) carbodimide hydrochloride (EDC). The combined beta-amylase and pullulanase activity was 32% relative to the nonimmobilized beta-amylase. Co-immobilization of beta-amylase and pullulanase increased the maltose yield compared to thart of the immobilized beta-amylase alone and reduced the saccharification time to about 50 h. The results showed that there is a significant increase in the thermal stability, pH stability, and stability toward gamma irradiation. The results also suggest that the co-immobilization of beta-amylase and pullulanase is a potentially useful approach for commercial starch hydrolysis.
authors.affiliation: Nuclear Research Center, Division of Radioisotopes Applications, Abo-Zabel, P.O. Box 13759, Cairo, Egypt.
authors.forename: K S
authors.initials: KS
authors.lastname: Atia
authors.forename: S A
authors.initials: SA
authors.lastname: Ismail
authors.forename: M B
authors.initials: MB
authors.lastname: El-Arnaouty
authors.forename: A M
authors.initials: AM
authors.lastname: Dessouki
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D003067:Coenzymes / Q000737:chemistry*; D004789:Enzyme Activation; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006026:Glycoside Hydrolases / Q000737:chemistry*; D006868:Hydrolysis; D008320:Maltose / Q000138:chemical synthesis*; D009097:Multienzyme Complexes / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013213:Starch / Q000737:chemistry*; D013696:Temperature; D001614:beta-Amylase / Q000737:chemistry*
chemical_list: D000180:Acrylic Resins; D003067:Coenzymes; D004800:Enzymes, Immobilized; D009097:Multienzyme Complexes; D008320:Maltose; D013213:Starch; D006026:Glycoside Hydrolases; D001614:beta-Amylase; C100181:pullulanase