Microarray analysis identifies interferon beta-regulated genes in multiple sclerosis.
The molecular mechanisms for the interferon beta (IFNbeta) treatment of multiple sclerosis (MS) remain to be characterized. Using cDNA microarray technology, we have compared the gene expression profile of T and non-T cells derived from relapsing-remitting MS before and after treatment with IFNbeta-1b. IFNbeta treatment significantly altered expression of 21 genes out of 1263 at 3 and 6 months after treatment. These genes included nine with IFN-responsive promoter elements. Whereas there was no change in Th1 or Th2 marker genes, some of the changes were unexpected but coincided with the beneficial effect of IFNbeta in MS.
Koike
Fumiko
F
Department of Immunology, National Institute of Neuroscience, NCNP, 4-1-1 Ogawahigashi, Tokyo 187-8502, Kodaira, Japan.
Satoh
Jun-ichi
J
Miyake
Sachiko
S
Yamamoto
Toshiyuki
T
Kawai
Mitsuru
M
Kikuchi
Seiji
S
Nomura
Kyouichi
K
Yokoyama
Kazumasa
K
Ota
Kohei
K
Kanda
Takashi
T
Fukazawa
Toshiyuki
T
Yamamura
Takashi
T
D000328:Adult; D000954:Antigens, Surface / Q000187:drug effects / Q000235:genetics / Q000276:immunology; D015415:Biomarkers / Q000097:blood; D016207:Cytokines / Q000187:drug effects / Q000235:genetics / Q000276:immunology; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000276:immunology; D005796:Genes / Q000187:drug effects* / Q000276:immunology; D006801:Humans; D016899:Interferon-beta / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000097:blood / Q000188:drug therapy* / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D018417:Th1 Cells / Q000187:drug effects / Q000276:immunology; D018418:Th2 Cells / Q000187:drug effects / Q000276:immunology; D016896:Treatment Outcome
D000954:Antigens, Surface; D015415:Biomarkers; D016207:Cytokines; D016899:Interferon-beta