title: Hydrogen peroxide production by alveolar macrophages is increased and its concentration is elevated in the breath of rats exposed to hypoxia: relationship to lung lipid peroxidation.
abstract: Hypoxic exposure triggers a generation of reactive oxygen species that initiate free radical damage to the lung. Hydrogen peroxide is the product of alveolar macrophages detectable in the expired breath. We evaluated the significance of breath H(2)O(2) concentration for the assessment of lung damage after hypoxic exposure and during posthypoxic period. Adult male rats were exposed to normobaric hypoxia (10 % O(2)) for 3 hours or 5 days. Immediately after the hypoxic exposure and then after 7 days or 14 days of air breathing, H(2)O(2) was determined in the breath condensate and in isolated lung macrophages. Lipid peroxidation was measured in lung homogenates. Three-hour hypoxia did not cause immediate increase in the breath H(2)O(2); 5-day hypoxia increased breath H(2)O(2) level to 458 %. After 7 days of subsequent air breathing H2O2 was elevated in both groups exposed to hypoxia. Increased production of H(2)O(2) by macrophages was observed after 5 days of hypoxia and during the 7 days of subsequent air breathing. Lipid peroxidation increased in the periods of enhanced H(2)O(2) generation by macrophages. As the major increase (1040 %) in the breath H(2)O(2) concentration found 7 days after 3 hours of hypoxia was not accompanied by lipid peroxidation, it can be concluded that the breath H(2)O(2) is not a reliable indicator of lung oxidative damage.
authors.affiliation: Department of Medical Chemistry and Biochemistry, Second Faculty of Medicine, Charles University, Prague, Czech Republic. jiri.wilhelm@lfmotol.cuni.cz
authors.forename: J
authors.initials: J
authors.lastname: Wilhelm
authors.forename: M
authors.initials: M
authors.lastname: Vanková
authors.forename: H
authors.initials: H
authors.lastname: Maxová
authors.forename: A
authors.initials: A
authors.lastname: Sisková
mesh_terms: D000447:Aldehydes / Q000032:analysis; D000704:Analysis of Variance; D000818:Animals; D001944:Breath Tests / Q000379:methods; D003627:Data Interpretation, Statistical; D006861:Hydrogen Peroxide / Q000032:analysis / Q000378:metabolism*; D000860:Hypoxia / Q000503:physiopathology*; D015227:Lipid Peroxidation; D008168:Lung / Q000378:metabolism / Q000503:physiopathology*; D016676:Macrophages, Alveolar / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012119:Respiration; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors
chemical_list: D000447:Aldehydes; D009240:N-Formylmethionine Leucyl-Phenylalanine; D006861:Hydrogen Peroxide; D013755:Tetradecanoylphorbol Acetate