Molecular cloning of equine muscle-type phosphofructokinase cDNA.
The complete coding region sequence of equine muscle-type phosphofructokinase (ePFKM) was obtained from skeletal muscle of a thoroughbred horse. The deduced amino acid sequence of ePFKM showed 97%, 96%, 96%, 96% and 95% identity to canine, human, mouse, rabbit and rat PFKM, respectively. The amino and carboxyl terminal halves of ePFKM presented a structure of tandem repeat, as other mammalian PFKMs. As the amino acid residues constituting various ligand-binding sites were also conserved, it is thought that ePFKM has enzymatic activity similar to PFKM in other mammals.
Sato
Tetsuo
T
Department of Preventive Veterinary Medicine and Animal Health, Nihon University School of Veterinary Medicine, Kanagawa, Japan.
Itou
Takuya
T
Sakai
Takeo
T
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D006736:Horses / Q000235:genetics*; D008969:Molecular Sequence Data; D025203:Phosphofructokinase-1, Muscle Type / Q000737:chemistry / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D025203:Phosphofructokinase-1, Muscle Type