[Effect of sappan wood on perforin mRNA expression in myocardium of rats after allogeneic cardiac transplantation].
OBJECTIVE
To observe the effect of Sappan wood (SW) on the expression of perforin mRNA in myocardium of rats after allogeneic cardiac transplantation.


METHODS
The animal model of allogeneic (abdominal) cardiac transplantation was established by taking Wistar rat as provider and SD rat as receptor, perforin mRNA expression in the model's myocardium was detected by RT-PCR.


RESULTS
SW could obviously reduce the perforin mRNA expression, it also could alleviate the pathological morphology and ultrastructural damage of myocardial cells.


CONCLUSIONS
SW has obvious effect in antagonizing immune rejection after transplantation, the mechanism of its immunosuppression could be through lowering the perforin mRNA expression.
Zhou
Ya-bin
YB
First Affiliated Hospital, Heilongjiang TCM University, Harbin 150040. zhouyabin@hotmail.com
Yao
Feng-zhen
FZ
Han
Jia-rui
JR
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007887:Fabaceae / Q000737:chemistry*; D016027:Heart Transplantation; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D054353:Perforin; D052899:Pore Forming Cytotoxic Proteins; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D014184:Transplantation, Homologous
D004365:Drugs, Chinese Herbal; D008562:Membrane Glycoproteins; D052899:Pore Forming Cytotoxic Proteins; D012333:RNA, Messenger; D054353:Perforin