title: [The overexpression of prion protein in drug resistant gastric cancer cell line SGC7901/ADR and its significance].
abstract: OBJECTIVE
  To investigate the overexpression of prion protein (PrP) in drug-resistant gastric cancer cell line SGC7901/ADR and its role in multidrug resistance in gastric cancer.
  
  
  METHODS
  (1) The expression of PrP in SGC7901/ADR, SGC790/VCR and their parental cell line SGC7901 was detected with Northern and Western blot at the mRNA and protein level. (2) Eukaryotic sense and antisense expression vector were constructed based on DNA recombination technology and (3) introduced into SGC7901 and SGC7901/ADR cell lines through electroporation. (4) The accumulation and retention of ADR in transiently transfected cells were detected by flow cytometry.
  
  
  RESULTS
  (1) Northern and western blot suggested significantly higher expression of PrP in SGC7901/ADR and SGC7901/VCR than that in SGC7901. (2) 48 hours after the vectors transfection, the average fluorescence intensity of Adr in transfected cells were detected. The accumulation intensity were 8.9 +/- 0.7 in BS, 6.6 +/- 0.3 in PS and 7.5 +/- 0.6 in PA. The retention intensity were 9.3 +/- 0.6 in SGC7901, 5.9 +/- 0.5 in PS and 7.1 +/- 0.5 in PA. There were significant difference between PS and BS with P < 0.01, as well as RA and BA with P < 0.01. These data suggested that PrP gene could affect the drug accumulation in gastric cancer cells after its transfected into cells.
  
  
  CONCLUSIONS
  PrP was highly expressed in gastric cancer cell lines SGC7901/ADR and SGC7901/VCR. Overexpression of PrP had certain effect on drug accumulation in gastric cancer cells.
authors.affiliation: Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi'an 710033, Shaanxi Province, China.
authors.forename: Jing-ping
authors.initials: JP
authors.lastname: Du
authors.forename: Xiao-hang
authors.initials: XH
authors.lastname: Jin
authors.forename: Yong-quan
authors.initials: YQ
authors.lastname: Shi
authors.forename: Yan-qiu
authors.initials: YQ
authors.lastname: Zhao
authors.forename: Chang-jiang
authors.initials: CJ
authors.lastname: Liu
authors.forename: Yun-xin
authors.initials: YX
authors.lastname: Cao
authors.forename: Tai-dong
authors.initials: TD
authors.lastname: Qiao
authors.forename: Bao-jun
authors.initials: BJ
authors.lastname: Chen
authors.forename: Dai-ming
authors.initials: DM
authors.lastname: Fan
mesh_terms: D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm; D006801:Humans; D011328:Prions / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D014162:Transfection
chemical_list: D011328:Prions; D012333:RNA, Messenger