title: True or apparent reversal of elution order during chiral high-performance liquid chromatography monitored by a polarimetric detector under different mobile phase conditions.
abstract: The use of all chromatographic modes (i.e. reversed-phase, normal-phase and polar organic modes) is becoming more and more systematic in chiral HPLC method development. When this method development is monitored by polarimetric detection, which is a widespread technique, one should not forget that the observed positive or negative rotation angle for a given absolute configuration depends on the solvent. As a cautionary illustration, a new example is reported for a racemate which is separated with the same elution order in ethanol or acetonitrile mobile phase on a CHIRALCEL OD-R column whereas the signs of polarimetric detection are reversed.
authors.affiliation: ENSSPICAM, Faculté Sciences St. Jérôme, UMR 6516, Université Aix-Marseille III, Avenue Escadrille Normandie-Niémen, 13397 Marseille Cedex 20, France. roussel@spi-chim.u-3mrs.fr
authors.forename: Christian
authors.initials: C
authors.lastname: Roussel
authors.forename: Nicolas
authors.initials: N
authors.lastname: Vanthuyne
authors.forename: Muriel
authors.initials: M
authors.lastname: Serradeil-Albalat
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Vallejos
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry / Q000295:instrumentation*; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism