Interleukin-4-expressing plasmid DNA inhibits reovirus type-2-triggered autoimmune insulitis in DBA/1 J suckling mice.
In this study we have examined the effect of systemic administration of T helper (Th) 2 cytokines on reovirus type-2 (Reo-2)-triggered Th1-mediated autoimmune insulitis with impaired glucose tolerance (IGT) in DBA/1J suckling mice. We have demonstrated clearly that the systemic administration of both interleukin (IL)-4-expressing plasmid DNA (pIL-4) and recombinant IL-4 (rIL-4) inhibited the development of insulitis with IGT in a dose dependent manner as compared to untreated groups in Reo-2-infected DBA/1J suckling mice. The inhibitory effects of IL-4 on the development of insulitis with IGT and the advantages of pIL-4 as compared to rIL-4 in this model are discussed.
Hayashi
T
T
Laboratory of Veterinary Pathology, Yamaguchi University, Yamaguchi, Japan. hayashi@agr.yamaguchi-u.ac.jp
Yasutomi
Y
Y
Hasegawa
K
K
Sasaki
Y
Y
Onodera
T
T
D000818:Animals; D000833:Animals, Suckling; D001327:Autoimmune Diseases / Q000473:pathology / Q000517:prevention & control* / Q000821:virology; D003921:Diabetes Mellitus, Experimental / Q000473:pathology / Q000517:prevention & control* / Q000821:virology; D005260:Female; D015316:Genetic Therapy / Q000379:methods*; D015847:Interleukin-4 / Q000235:genetics / Q000378:metabolism* / Q000627:therapeutic use; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D030102:Orthoreovirus, Mammalian; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000627:therapeutic use; D012088:Reoviridae Infections / Q000150:complications
D011994:Recombinant Proteins; D015847:Interleukin-4