Effects of the CDT6/ANGX gene on tumour growth in immune competent mice.
BACKGROUND
Cornea-derived transcript 6 (CDT6, also known as AngX) has been described to inhibit tumour growth in a human melanoma growing in nude mice. In another report, however, enhancement of tumour growth in comparable circumstances occurred. We therefore studied the effect of the same gene in different circumstances, using an immune competent mouse model.


METHODS
In this report we describe the generation of a stably CDT6-expressing clone of the murine melanoma cell line B16-F10. These cells were implanted in female C57/B16 mice with empty vector transfected cells as control.


RESULTS
We found no significant inhibition or stimulation of either lag-time or doubling-time of the tumours. In addition no effect of the CDT6 gene product was found on proliferation of endothelial cells in vitro.


CONCLUSIONS
In contrast to an immune deficient mouse model, no anti-tumour effect could be detected with the CDT6 gene product in an immune competent mouse model.
Bouïs
Diane
D
Department of Medical Oncology, University Hospital Groningen, P.O. Box 30001, 9700 RB Groningen, The Netherlands.
Hospers
Geke A P
GA
Meijer
Coby
C
Dam
Wendy
W
Peek
Ron
R
Mulder
Nanno H
NH
D043925:Angiogenesis Inducing Agents / Q000627:therapeutic use*; D042501:Angiogenic Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D004730:Endothelium, Vascular / Q000187:drug effects / Q000473:pathology; D005260:Female; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D007121:Immunocompetence; D008546:Melanoma, Experimental / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D012333:RNA, Messenger / Q000378:metabolism; D012334:RNA, Neoplasm / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
D043925:Angiogenesis Inducing Agents; D042501:Angiogenic Proteins; D000970:Antineoplastic Agents; D012333:RNA, Messenger; D012334:RNA, Neoplasm