title: Canine X-linked muscular dystrophy in Japan (CXMDJ).
abstract: The purpose of this study was to develop a strain of canine X-linked muscular dystrophy (CXMD), a model of Duchenne muscular dystrophy, in Japan. A female beagle was artificially inseminated with frozen-thawed spermatozoa derived from an affected golden retriever. Subsequently, two carrier female dogs (G1 carriers) and four normal male littermates were produced. Thereafter, the two G1 carriers were mated with beagle sires. As a result, each bitch whelped three times, and out of 54 pups, 17 affected male descendants, and 11 carrier female descendants (G2 carriers) were detected. One G2 carrier was then mated with a beagle sire and 15 pups in two whelpings were produced, including five affected males and four carrier females (G3 carriers). A total of 10 female beagles were artificially inseminated to evaluate the fertility of the frozen-thawed spermatozoa from the two affected dogs. The whelping rates of the two affected dogs were 4/5 and the litter sizes were 5.0 +/- 1.41 and 6.0 +/- 0.82, respectively. These results indicate that a canine X-linked muscular dystrophy colony has been established in Japan. We called them CXMDJ.
authors.affiliation: Chugai Research Institute for Medical Science, Inc., 6598 Toyoda, Suwa, Nagano 392-0016, Japan.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Shimatsu
authors.forename: Kouichi
authors.initials: K
authors.lastname: Katagiri
authors.forename: Toshio
authors.initials: T
authors.lastname: Furuta
authors.forename: Masao
authors.initials: M
authors.lastname: Nakura
authors.forename: Yoshikuni
authors.initials: Y
authors.lastname: Tanioka
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Yuasa
authors.forename: Masayuki
authors.initials: M
authors.lastname: Tomohiro
authors.forename: Joe N
authors.initials: JN
authors.lastname: Kornegay
authors.forename: Ikuya
authors.initials: I
authors.lastname: Nonaka
authors.forename: Shin'ichi
authors.initials: S
authors.lastname: Takeda
mesh_terms: D000818:Animals; D004285:Dogs; D005260:Female; D008040:Genetic Linkage; D005838:Genotype; D007564:Japan; D008297:Male; D009136:Muscular Dystrophies / Q000235:genetics*; D010375:Pedigree; D014960:X Chromosome