Notch1 enhances B-cell receptor-induced apoptosis in mature activated B cells without affecting cell cycle progression and surface IgM expression.
The transmembrane receptor Notch1 plays a crucial role in differentiation and apoptosis of hematopoietic cells. To investigate the influence of Notch1 on apoptosis and cell growth of mature murine B cells, we transduced the murine B-lymphoma line NYC 31.1 with a constitutively active, intracellular form of human Notch1 (Notch1-ICT). NYC cells represent mature activated B cells that can be induced to undergo apoptosis by crosslinking of the B-cell receptor (BCR). In contrast to investigations in immature chicken B-cell lines, transduced Notch1-ICT did not affect cell cycle progression, cell growth or surface IgM levels in NYC cells and resulted only in a slight induction of apoptosis. However, BCR-crosslinking enhanced apoptosis, but did not influence cell cycle progression in Notch1-ICT-positive NYC cells. These data imply a distinct function of Notch1 in mature murine B-cells as compared to immature chicken B cells and provide further evidence for Notch1's involvement in B-cell differentiation and development.
Romer
S
S
Division of Molecular Immunology, Department of Internal Medicine III, Nikolaus-Fiebiger Center, Friedrich-Alexander-University Erlangen-Nürnberg, Glückstrasse 6, 91054 Erlangen, Germany.
Saunders
U
U
Jäck
H-M
HM
Jehn
B M
BM
D000818:Animals; D017209:Apoptosis / Q000235:genetics / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D002453:Cell Cycle / Q000235:genetics / Q000276:immunology; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D045744:Cell Line, Tumor; D002645:Chickens; D006410:Hematopoiesis / Q000235:genetics / Q000276:immunology; D007075:Immunoglobulin M / Q000378:metabolism*; D051379:Mice; D051881:Receptor, Notch1; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity; D014157:Transcription Factors; D014161:Transduction, Genetic; D015854:Up-Regulation / Q000235:genetics / Q000276:immunology
D007075:Immunoglobulin M; C497762:Notch1 protein, mouse; D051881:Receptor, Notch1; D011947:Receptors, Antigen, B-Cell; D011956:Receptors, Cell Surface; D014157:Transcription Factors