Bioconjugated nanoparticles for DNA protection from cleavage.
We have developed a novel method to protect DNA from cleavage using bioconjugated nanoparticles. Positively charged amino-modified silica nanoparticles have been directly prepared using water-in-oil microemulsion. Plasmid DNA can be easily enriched onto the positively charged nanoparticle surface, and the DNA strands are well protected from enzymatic cleavage. When incubated with nuclease enzyme for enzymatic cleavage, free plasmid DNA strands are completely cleaved, while those on the nanoparticle surfaces are intact. Our results clearly demonstrate unique properties of nanomaterials when combined with biomolecules. Our simple bionanotechnology will be highly useful in DNA separation, manipulation, and detection, and possibly in genetic engineering and gene therapy, as plasmid DNA can be protected in cellular environments without any change in its property.
He
Xiao-xiao
XX
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry & Chemical Engineering, Institute of Biological Technology, Hunan University, Changsha 410082, P. R. China.
Wang
Kemin
K
Tan
Weihong
W
Liu
Bin
B
Lin
Xia
X
He
Chunmei
C
Li
Du
D
Huang
Shasheng
S
Li
Jun
J
D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000737:chemistry / Q000378:metabolism; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000737:chemistry / Q000235:genetics; D008856:Microscopy, Fluorescence; D036103:Nanotechnology / Q000379:methods; D010316:Particle Size; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D012822:Silicon Dioxide / Q000737:chemistry*
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins; D012822:Silicon Dioxide; D004247:DNA; D003850:Deoxyribonuclease I