Covalently coupling the antibody on an amine-self-assembled gold surface to probe hyaluronan-binding protein with capacitance measurement.
Hyaluronan-binding proteins (HABPs), the important structural components of extracellular matrices, served important structural and regulatory functions during development and in maintaining adult tissue homestats. A sensitive, specific and rapid-responsing immunosensor to probe hyaluronan-binding cartilage protein was presented in this work. The novel immunosensor supplied a label-free detection method for HABP, which was based on measuring the capacitance change in-between the unlabeled HABP (antigen) and rabbit-anti-HABP (Ra-HABP, antibody). The HABP immunosensor was prepared by covalently coupling Ra-HABP on an amine-self-assembled gold surface with glutaraldehyde. The capacitance change corresponding to the concentration of HABP, the target antigen, was evaluated by an electrochemical approach called potentiostatic-step in microseconds. The immunosensor showed a specific response to HABP in the range 10-1000 ng/ml. The presented work supplied a promising clinical screening method.
Jiang
Dechen
D
Department of Chemistry, Fudan University, 200433, Shanghai, People's Republic of China.
Tang
Jia
J
Liu
Baohong
B
Yang
Pengyuan
P
Shen
Xianrong
X
Kong
Jilie
J
D000327:Adsorption; D000588:Amines / Q000737:chemistry; D000906:Antibodies / Q000032:analysis / Q000737:chemistry* / Q000276:immunology; D000936:Antigen-Antibody Complex / Q000032:analysis; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry*; D025802:Electric Capacitance; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry*; D018960:Hyaluronan Receptors / Q000032:analysis* / Q000276:immunology; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000588:Amines; D000906:Antibodies; D000936:Antigen-Antibody Complex; D020099:Coated Materials, Biocompatible; D018960:Hyaluronan Receptors; D006046:Gold