title: A comparison of the phenotype of dendritic cells derived from discrete Peyer's patch macrophages of non-infected and Toxoplasma gondii infected mice.
abstract: A comparison of the expression of surface membrane antigens between dendritic cells (DC) derived from Peyer's patch macrophages (DPP-DC) of non-infected and Toxoplasma gondii (T. gondii) infected mice was performed. C57BL/6J mice aged 6-8 weeks of both sexes were infected orally with a 0.5 ml suspension containing 2 x 10(4) bradyzoites of the Beverley strain of T. gondii, sacrificed on day 8 and DC generated using discrete Peyer's patch macrophages (DPP-Mø) as progenitor cells. When a comparison of the expression of surface membrane antigens between the antigen presenting cells (APC) obtained from discrete Peyer's patches of non-infected and T. gondii infected mice was carried out, no significant differences were observed in the macrophage progenitor and DC populations expression of F4/80, DEC-205, CD11c, CD80 (B7-1) and CD34. However, a significant decrease in MHC class II antigen levels and a down regulation of the co-stimulatory molecule CD86 (B7-2) were noted. B7-1 appeared to be the dominant co-stimulatory ligand, whereas B7-2, which was down regulated during T. gondii infection, had a weak expression. Taken together, these results may help clarify the role of DC in the complex network regulating surface membrane antigens, as well as, their capacity for antigen uptake, processing and presentation during toxoplasmosis.
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
authors.forename: Levi H C
authors.initials: LH
authors.lastname: Makala
authors.forename: Julio C S
authors.initials: JC
authors.lastname: Reyes
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Nishikawa
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Tsushima
authors.forename: Xuenan
authors.initials: X
authors.lastname: Xuan
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Huang
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Nagasawa
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism; D000953:Antigens, Protozoan / Q000276:immunology; D018122:B7-1 Antigen / Q000276:immunology / Q000378:metabolism; D051940:B7-2 Antigen; D002470:Cell Survival; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology*; D005260:Female; D005786:Gene Expression Regulation; D000949:Histocompatibility Antigens Class II / Q000276:immunology / Q000378:metabolism; D016130:Immunophenotyping; D008264:Macrophages / Q000166:cytology*; D008297:Male; D008562:Membrane Glycoproteins / Q000276:immunology / Q000378:metabolism; D051379:Mice; D010581:Peyer's Patches / Q000166:cytology* / Q000276:immunology; D014122:Toxoplasma / Q000502:physiology*; D014123:Toxoplasmosis / Q000276:immunology*
chemical_list: D015703:Antigens, CD; D000953:Antigens, Protozoan; D018122:B7-1 Antigen; D051940:B7-2 Antigen; C497965:Cd86 protein, mouse; D000949:Histocompatibility Antigens Class II; D008562:Membrane Glycoproteins