Pentanucleotide TTTTA and G/A -914 DNA polymorphisms in apolipoprotein(a) promoter: genotyping by single-tube PCR.
It is known that pentanucleotide repeat polymorphism, consisting of varying number of (TTTTA) repeats in a promoter, is associated with plasma lipoprotein(a) levels. G/A -914 base substitution has also been correlated with pentanucleotide repeat polymorphism in individuals with high and low lipoprotein(a) levels. To facilitate future analysis of these polymorphisms we have developed a quick and reliable assay for the detection of both polymorphisms in a single polymerase chain reaction.
Zivković
Maja
M
VINCA Institute of Nuclear Sciences, Laboratory for Radiobiology and Molecular Genetics, Belgrade, Serbia, Yugoslavia.
Stanković
Aleksandra
A
Alavantić
Dragan
D
D001053:Apolipoproteins / Q000097:blood* / Q000235:genetics*; D053241:Apoprotein(a); D004247:DNA / Q000097:blood* / Q000235:genetics*; D005838:Genotype; D006801:Humans; D017270:Lipoprotein(a) / Q000097:blood* / Q000235:genetics*; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D001053:Apolipoproteins; D017270:Lipoprotein(a); D004247:DNA; D053241:Apoprotein(a)