title: Effect of ethanol intake during lactation on male and female pups' liver and brain metabolism during the suckling-weaning transition period.
abstract: In rats, a high degree of brain development and myelination occurs during the first 15 days after birth. Ethanol intake by lactating rats modified 12 day-old pups' brain development and metabolism. The aim of the present study was to evaluate the effect of maternal ethanol ingestion during lactation on prepubertal (24-day-old) pups' brain and liver metabolism. Lactating rats (4 male and 4 female litters) were divided into 2 groups: control--received control liquid diet, and ethanol--received liquid diet containing 4% of ethanol. On postnatal day 24, the pups were killed by decapitation. Liver and brain were utilized for measuring Adenosine Tri-phosphate-citrate lyase and malic enzymes activities. Brain slices were incubated in medium containing glucose to determine glucose consumption and oxidation, and lipid synthesis. The ethanol intake decreased male and female pups' body, brain and liver weight. Liver Adenosine Tri-phosphate-citrate lyase activity was decreased only in male pups of the ethanol group. The intake of ethanol solution by the dams increased glucose consumption and oxidation by the incubated female pups' brain slices and decreased glucose oxidation by the male pups' brain slices. It can be concluded that the effects of maternal ethanol intake on pups' development and metabolism are gender-related.
authors.affiliation: Departamento de Fisiologia, Disciplina de Neurofisiologia e Fisiologia Endócrina, Universidade Federal de São Paulo, EPM, Rua Botucatu, 862, 2nd floor, Edificio de Ciências Biomédicas, Vila Clementino, São Paulo, SP 04023-060, Brazil. lila@ecb.epm.br
authors.forename: L M
authors.initials: LM
authors.lastname: Oyama
authors.forename: C M
authors.initials: CM
authors.lastname: Oller Do Nascimento
mesh_terms: D001275:ATP Citrate (pro-S)-Lyase / Q000378:metabolism; D000818:Animals; D000833:Animals, Suckling; D001835:Body Weight / Q000187:drug effects; D001921:Brain / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D000431:Ethanol / Q000008:administration & dosage*; D005260:Female; D005947:Glucose / Q000378:metabolism; D007774:Lactation; D008099:Liver / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D008291:Malate Dehydrogenase / Q000378:metabolism; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D012727:Sex Characteristics; D014886:Weaning
chemical_list: D000431:Ethanol; D008291:Malate Dehydrogenase; D001275:ATP Citrate (pro-S)-Lyase; D005947:Glucose