title: Occurrence of extended-spectrum beta-lactamases among Escherichia coli isolates from hospitalized and healthy children.
abstract: The prevalence of extended-spectrum beta-lactamases (ESBL) was determined among isolates of Escherichia coli (n = 63) isolated from hospitalized (43) and healthy (20) children. Ten isolates (21%) were ESBL-positive for two screening tests, the double disk-synergy test and the Oxoid Combination Disk method. One ESBL-positive isolate came from a healthy child. The transfer frequency of oxyimino-beta-lactam resistance from ESBL-producing isolates to E. coli K12 C600 recipient strain ranged from 10(-8) to 10(-5) per donor cell. Donor strains and transconjugants displayed susceptibility patterns typical of ESBL producers. They were resistant to oxyimino-beta-lactams but susceptible to clavulanic acid and carbapenems. Seven out of the 10 ESBL-positive isolates were found to produce MR/MS fimbria, which may play an important role in the colonization of the human intestinal mucosa.
authors.affiliation: Department of Microbiology, University of Medicine in Wrocław, 50 368 Wrocław, Poland. romanf@mbio.am.wroc.pl
authors.forename: R
authors.initials: R
authors.lastname: Franiczek
authors.forename: B
authors.initials: B
authors.lastname: Sobieszczańska
authors.forename: M
authors.initials: M
authors.lastname: Grabowski
authors.forename: K
authors.initials: K
authors.lastname: Mowszet
authors.forename: T
authors.initials: T
authors.lastname: Pytrus
mesh_terms: D002648:Child; D002675:Child, Preschool; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology*; D004926:Escherichia coli / Q000145:classification / Q000201:enzymology / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology*; D006760:Hospitalization; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000378:metabolism*; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D047090:beta-Lactams; D001618:beta-Lactamases