title: Ionizing radiation sensitivity of Listeria monocytogenes ATCC 49594 and Listeria innocua ATCC 51742 inoculated on endive (Cichorium endiva).
abstract: Ionizing radiation inactivates the pathogenic bacteria that can contaminate leafy green vegetables. Leaf pieces and leaf homogenate of endive (Cichorium endiva) were inoculated with the pathogen Listeria monocytogenes (ATCC 49594) or Listeria innocua (ATCC 51742), a nonpathogenic surrogate bacterium. The radiation sensitivity of the two strains was similar, although L. innocua was more sensitive to the type of suspending leaf preparation. During refrigerated storage after irradiation, the population of L. monocytogenes on inoculated endive was briefly suppressed by 0.42 kilogray (kGy), a dose calibrated to achieve a 99% reduction. However, the pathogen regrew after 5 days until it exceeded the bacterial levels on the control after 19 days in storage. Treatment with 0.84 kGy, equivalent to a 99.99% reduction, suppressed L. monocytogenes throughout refrigerated storage. Doses up to 1.0 kGy had no significant effect on the color of endive leaf material, regardless of whether taken from the leaf edge or the leaf midrib. The texture of leaf edge material was unaffected by doses up to 1.0 kGy, whereas the maximum dose tolerated by leaf midrib material was 0.8 kGy. These results show that endive leaves may be treated with doses sufficient to achieve at least a 99.99% reduction of L. monocytogenes with little or no impact on the product's texture or color.
authors.affiliation: U.S. Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 East Mermaid Lane, Wyndmoor, Pennsylvania 19038, USA. bniemira@arserrc.gov
authors.forename: Brendan A
authors.initials: BA
authors.lastname: Niemira
authors.forename: Xuetong
authors.initials: X
authors.lastname: Fan
authors.forename: Kimberly J B
authors.initials: KJ
authors.lastname: Sokorai
authors.forename: Christopher H
authors.initials: CH
authors.lastname: Sommers
mesh_terms: D019659:Asteraceae / Q000382:microbiology* / Q000528:radiation effects; D015169:Colony Count, Microbial; D004307:Dose-Response Relationship, Radiation; D005511:Food Handling / Q000379:methods; D005514:Food Irradiation; D008087:Listeria / Q000254:growth & development / Q000528:radiation effects; D008089:Listeria monocytogenes / Q000254:growth & development / Q000528:radiation effects*; D018515:Plant Leaves / Q000382:microbiology; D012680:Sensitivity and Specificity; D013997:Time Factors; D014675:Vegetables / Q000382:microbiology* / Q000528:radiation effects / Q000592:standards