Genome analysis of a novel Shiga toxin 1 (Stx1)-converting phage which is closely related to Stx2-converting phages but not to other Stx1-converting phages.
Two Stx-converting phages, designated Stx1 phi and Stx2 phi-II, were isolated from an Escherichia coli O157:H7 strain, Morioka V526, and their entire nucleotide sequences were determined. The genomes of both phages were similar except for the stx gene-flanking regions. Comparing these phages to other known Stx-converting phages, we concluded that Stx1 phi is a novel Stx1-converting phage closely related to Stx2-converting phages so far reported.
Sato
Toshio
T
Research Institute, International Medical Center of Japan, Shinjuku, Tokyo 162-8655, Japan. tsato@ri.imcj.go.jp
Shimizu
Takeshi
T
Watarai
Masahisa
M
Kobayashi
Midori
M
Kano
Shigeyuki
S
Hamabata
Takashi
T
Takeda
Yoshifumi
Y
Yamasaki
Shinji
S
D003090:Coliphages / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D019453:Escherichia coli O157 / Q000821:virology*; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D017422:Sequence Analysis, DNA; D022622:Shiga Toxin 1 / Q000235:genetics / Q000378:metabolism*; D022641:Shiga Toxin 2 / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics
D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; D014764:Viral Proteins