Reversal of multidrug resistance in mouse lymphoma cells by phenothiazines.
Various compounds were tested with regard to their reversal of multidrug resistance (MDR) in mouse tumor cells transfected with the human MDR1 gene. Phenothiazines containing aromatic moieties were bound through stacking interaction involving the polarization of the aromatic aminoacid substituents at the target site of p-glycoprotein (Pgp) 170, as a consequence of their large dipoles (as in the binding of phenothiazine to calmodulin-like structures). Acting as a calcium channel blocker, verapamil may induce conformational changes in the calcium channel-like structures of the transmembrane regions of Pgp. Most probably the tyrosine moieties of Pgp are involved in the action of verapamil and phenothiazines. Tomato lectin specifically binds to the polylactosamine moiety of Pgp170 at the first loop of Pgp. Other targets in the membrane may exist in close proximity to Pgp170, such as conA-reactive glycoproteins with terminal mannosyl residues. WGA-reactive N-acetyl glucosamine residues can also be modified resulting in conformational changes in trans-membrane regions of the ABC transporter. Our results demonstrate that MDR can be reversed by interaction of various compounds with Pgp or by modification of the membrane structure around the Pgp.
Molnar
Joseph
J
Department of Microbiology, Albert Szent-Györgyi Medical University, Szeged, Hungary. molnarj@comser.szote.u-szeged.hu
Molnar
Annamaria
A
Mucsi
Ilona
I
Pinter
Oliver
O
Nagy
Beatrix
B
Varga
Andreas
A
Motohashi
Noboru
N
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D002121:Calcium Channel Blockers / Q000494:pharmacology; D018432:Drug Resistance, Multiple / Q000187:drug effects*; D004359:Drug Therapy, Combination; D005434:Flow Cytometry; D007940:Leukemia L5178 / Q000188:drug therapy* / Q000378:metabolism; D051379:Mice; D010640:Phenothiazines / Q000494:pharmacology*; D014700:Verapamil / Q000494:pharmacology
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000970:Antineoplastic Agents; D002121:Calcium Channel Blockers; D010640:Phenothiazines; D014700:Verapamil