There is no evidence for the existence of complex formation between doxorubicin and glutathione.
Doxorubicin is co-transported with glutathione by several multidrug resistance proteins (MRPs). In order to check whether weak non-covalent aggregates between doxorubicin and glutathione can be formed, which might be substrates for the transporter, the effect of glutathione on the partition coefficient of doxorubicin was studied. No evidence of an effect of glutathione (at levels up to 20 microM) on the partition coefficient of doxorubicin was found in the pH range of 4.0-7.4. These results indicate that non-covalent doxorubicin-glutathione complexes do not form.
Marszałek
Małgorzata
M
Department of Molecular Biophysics, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland.
Bartosz
Małgorzata
M
Bartosz
Grzegorz
G
D000255:Adenosine Triphosphate; D004317:Doxorubicin / Q000378:metabolism*; D005453:Fluorescence; D005978:Glutathione / Q000378:metabolism*
D004317:Doxorubicin; D000255:Adenosine Triphosphate; D005978:Glutathione