Establishment of transgenic mice carrying the gene of human nuclear receptor NR5A2 (hB1F).
OBJECTIVE
Human hepatitis B virus enhancer II B1 binding factor (hB1F) was cloned and characterized as a novel member of the Ftz-F1 (NR5A) nuclear receptor subfamily. Although progresses have recently been made, its biological function remains largely unidentified. The aim of this study was to establish an hB1F transgenic mouse model to promote the functional study of hB1F.


METHODS
Transgene fragments were microinjected into fertilized eggs of mice. The manipulated embryos were transferred into the oviducts of pseudopregnant female mice. The offsprings were identified by PCR and Southern blot analysis. Transgene expression was analyzed with RT-PCR and Western blot analysis. Transgenic founder mice were used to establish transgenic mouse lineages. The F1 and F2 mice were identified by PCR analysis.


RESULTS
Seven mice were identified as carrying copies of transgene. RT-PCR and Western blotting results showed that the transgene was expressed in heart, liver, lung, kidney and stomach in one of the transgenic mouse lineages. Genetic analysis of the transgenic mice demonstrated that the transgene was integrated into the chromosome at a single site, and was transmitted stably.


CONCLUSIONS
In this study we established an hB1F transgenic mouse model, which will facilitate the investigation of the biological function of hB1F in vivo.
Wang
Shui-Liang
SL
Department of Medical Genetics, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China.
Yang
Hua
H
Xie
You-Hua
YH
Wang
Yuan
Y
Li
Jian-Zhong
JZ
Wang
Long
L
Wang
Zhu-Gang
ZG
Fu
Ji-Liang
JL
D000818:Animals; D004268:DNA-Binding Proteins; D005260:Female; D015870:Gene Expression; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic / Q000235:genetics*; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics*; D014018:Tissue Distribution; D014157:Transcription Factors; D019076:Transgenes
D004268:DNA-Binding Proteins; C082631:NR5A2 protein, human; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors