title: Multiple activities of a novel substance, dictyopyrone C isolated from Dictyostelium discoideum, in cellular growth and differentiation.
abstract: We report that a novel substance named dictyopyrone C (DPC) has remarkable effects on growth and differentiation of Dictyostelium discoideum Ax-2 cells, in a dose-dependent manner. In the presence of 3-15 microM DPC, differentiation of starving Ax-2 (clone MS) cells was greatly enhanced in submerged culture, when vegetative MS cells were harvested at the mid-late-exponential growth phase (>3 x 10(6) cells per ml) and starved. In contrast, DPC above 30 microM markedly impaired the progression of differentiation including cell aggregation, most of starved cells being round after 3-4 h of DPC application and then lysed during further incubation. In the presence of 30 microM DPC however, MS cells that had been harvested at the early exponential growth phase (<5 x 10(5) cells per ml) and starved became neither round nor lysed and exhibited rather enhanced differentiation. Essentially the same results were obtained in cultures of starved cells on nonnutrient agar. With respect to the DPC effect on MS cells growing in axenic medium, cell lysis and growth inhibition by DPC at concentrations higher than 15 microM were realized in the mid-late-exponential-growth-phase cells (>3 x 10(6) cells per ml) but not in the early-exponential-growth-phase cells (<5 x 10(5) cells per ml). Moreover, analysis using synchronized MS cells has demonstrated that the DPC effect changes in a cell-cycle-dependent manner. In contrast to such unique DPC actions, the pyrone ring of DPC had no effects on growth and differentiation within the range of 3-120 microM tested. These findings strongly suggested the importance of the combined structure of the pyrone ring and the linear carbon chain in revelation of the DPC activities.
authors.affiliation: Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Aoba, Sendai, Japan. ymaeda@mail.cc.tohoku.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Maeda
authors.forename: H
authors.initials: H
authors.lastname: Kikuchi
authors.forename: K
authors.initials: K
authors.lastname: Sasaki
authors.forename: A
authors.initials: A
authors.lastname: Amagai
authors.forename: J
authors.initials: J
authors.lastname: Sekiya
authors.forename: Y
authors.initials: Y
authors.lastname: Takaya
authors.forename: Y
authors.initials: Y
authors.lastname: Oshima
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002453:Cell Cycle; D002454:Cell Differentiation; D004023:Dictyostelium / Q000737:chemistry* / Q000254:growth & development*; D006131:Growth Inhibitors / Q000502:physiology; D006133:Growth Substances / Q000502:physiology*; D011753:Pyrones / Q000378:metabolism* / Q000494:pharmacology*
chemical_list: D006131:Growth Inhibitors; D006133:Growth Substances; D011753:Pyrones; C482605:dictyopyrone C