Germ-line transgenesis of the Tc1/mariner superfamily transposon Minos in Ciona intestinalis.
The tadpole larva of the basal chordate Ciona intestinalis has the most simplified, basic body-plan of chordates. Because it has a compact genome with a complete draft sequence, a large quantity of EST/cDNA information, and a short generation time, Ciona is a suitable model for future genetics. We establish here a transgenic technique in Ciona that uses the Tc1/mariner superfamily transposon Minos. Minos was integrated efficiently into the genome of germ cells and transmitted stably to subsequent generations. In addition, an enhancer-trap line was obtained. This is a demonstration of efficient, Minos-mediated transgenesis in marine invertebrates.
Sasakura
Yasunori
Y
Department of Zoology, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan. sasakura@ascidian.zool.kyotou.ac.jp
Awazu
Satoko
S
Chiba
Shota
S
Satoh
Nori
N
D000818:Animals; D030801:Animals, Genetically Modified; D015139:Blotting, Southern; D002938:Ciona intestinalis / Q000235:genetics*; D004251:DNA Transposable Elements; D018076:DNA, Complementary / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics*; D020224:Expressed Sequence Tags; D005821:Genetic Techniques; D005822:Genetic Vectors; D016678:Genome; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000378:metabolism; D008957:Models, Genetic; D016133:Polymerase Chain Reaction; D019895:Transposases / Q000235:genetics*
D004251:DNA Transposable Elements; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D008164:Luminescent Proteins; C111652:mariner transposases; D049452:Green Fluorescent Proteins; D019895:Transposases