Enhancement of antral contractions and vagal afferent signaling with synchronized electrical stimulation.
Gastric filling activates vagal afferents involved in peripheral signaling to the central nervous system (CNS) for food intake. It is not known whether these afferents linearly encode increasing contractions of the antrum during antral distension (AD). The aim of this study was to investigate effects of AD and electrically enhanced antral contractions on responses of vagal afferents innervating the antrum. Single-fiber recordings were made from the vagal afferents in anesthetized male Long-Evans rats. Antral contractions were measured with a solid-state probe placed in the antrum. A nonexcitatory electrical stimulation (NES) inducing no smooth muscle contractions was applied during the ascending phase of antral contractions to enhance subsequent antral contractions. Fifty-six fibers identified during AD (1 ml for 30 s) were studied through different types of mechanical stimuli. Under normal conditions, one group of fibers exhibited rhythmic firing in phase with antral contractions. Another group of fibers had nonrhythmic spontaneous firing. Responses of 15 fibers were tested with NES during multiple-step distension (MSD). NES produced a mean increase in antral contraction amplitude (177.1 +/- 35.3%) and vagal afferent firing (21.6 +/- 2.6%). Results show that both passive distension and enhanced antral contractions activate distension-sensitive vagal afferents. Responses of these fibers increase linearly to enhanced antral contraction induced by NES or MSD up to a distending volume of 0.6 ml. However, responses reached a plateau at a distending volume >0.8 ml. We concluded that enhanced contraction of the antrum can activate vagal afferents signaling to the CNS.
Peles
Shachar
S
Cardiovascular Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
Petersen
Jaime
J
Aviv
Ricardo
R
Policker
Shai
S
Abu-Hatoum
Ossama
O
Ben-Haim
Shlomo A
SA
Gutterman
David D
DD
Sengupta
Jyoti N
JN
D000818:Animals; D002404:Catheterization; D003655:Decerebrate State; D004558:Electric Stimulation / Q000379:methods; D004594:Electrophysiology; D005769:Gastrointestinal Motility / Q000502:physiology*; D008297:Male; D009424:Nervous System Physiological Phenomena; D009475:Neurons, Afferent / Q000502:physiology*; D011312:Pressure; D011706:Pyloric Antrum / Q000294:innervation / Q000502:physiology*; D051381:Rats; D020318:Rats, Long-Evans; D015398:Signal Transduction; D014630:Vagus Nerve / Q000502:physiology*