title: [Changes of STAT3 in cell replicative senescence and effects of angiotensin II on STAT3].
abstract: OBJECTIVE
  To investigate the changes of STAT3 in cell replicative senescence and the effects of angiotensin II (AngII) on STAT3.
  
  
  METHODS
  Normal human fetal lung diploid fibroblast cell line WI-38 was cultured and the senescent cell was defined with beta-gal staining. Electrophoretic mobility shift assay and Western blotting assay were used to detect the activation of STAT3 during cell replicative senescence and examine the process of STAT3 activation with AngII.
  
  
  RESULTS
  The synthesis of STAT3 protein during the course of cell replicative senescent increased, the nuclear translocation of phosphorylated STAT3 decreased, and the activities of STAT3 binding DNA reduced.
  
  
  CONCLUSIONS
  AngII induces the activation of STAT3 in both mature and senescence cell by AT(1) receptor, however, the reaction of senescent cell to AngII is weaker. The site of block may be in the nuclear translocation. AngII induces the activity of STAT3 in replicative senescence WI-38.
authors.affiliation: Department of Nephrology, General Hospital of the People's Liberation Army, Beijing 100853, China.
authors.forename: Xiao-dan
authors.initials: XD
authors.lastname: Wang
authors.forename: Xiang-mei
authors.initials: XM
authors.lastname: Chen
authors.forename: Feng
authors.initials: F
authors.lastname: Zhou
authors.forename: Bo
authors.initials: B
authors.lastname: Fu
authors.forename: Quan
authors.initials: Q
authors.lastname: Hong
authors.forename: Zhe
authors.initials: Z
authors.lastname: Feng
authors.forename: Jian-zhong
authors.initials: JZ
authors.lastname: Wang
authors.forename: Dai-ming
authors.initials: DM
authors.lastname: Fan
mesh_terms: D021581:Active Transport, Cell Nucleus; D000804:Angiotensin II / Q000494:pharmacology*; D002460:Cell Line; D016922:Cellular Senescence / Q000502:physiology*; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D006801:Humans; D010766:Phosphorylation; D050796:STAT3 Transcription Factor; D015534:Trans-Activators / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D015534:Trans-Activators; D000804:Angiotensin II; D004247:DNA; D001616:beta-Galactosidase