Improved serodiagnosis of erythema migrans using novel recombinant borrelial BBK32 antigens.
The performances of recombinant borrelial BBK32 proteins as antigens in the serology of erythema migrans (EM) were evaluated in an ELISA. Serum samples were obtained from 75 patients from different geographic areas where three borrelial species, Borrelia burgdorferi sensu stricto, Borrelia afzelii or Borrelia garinii, cause Lyme borreliosis. Antibodies to variant BBK32 proteins were compared with anti-flagella or with anti-IR(6) peptide antibodies. In IgG ELISA at presentation of EM, 65/75 (87 %) patients had antibodies to one or more variants of BBK32, 29/75 (39 %) had antibodies to flagella and 29/75 (39 %) had antibodies to the VlsE IR(6) peptide antigen. The immunoreactivity against variant BBK32 proteins differed in patients from different geographic regions. The present results suggest that the BBK32 proteins used in combination or in parallel may improve the laboratory diagnosis of EM.
Lahdenne
Pekka
P
Hospital for Children and Adolescents, University of Helsinki, Stenbäckinkatu 11, 00290 Helsinki, Finland 2Department of Bacteriology and Immunology, Haartman Institute, Haartmaninkatu 3, 00014 Helsinki, Finland 3,7Department of Otolaryngology3 and Laboratory Diagnostics7, Helsinki University Central Hospital, 00290 Helsinki, Finland 4Division of Rheumatology, Allergy and Immunology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA 5Department of Infectious Diseases, University Medical Center Ljubljana, Slovenia 6Professor-Hess-Children's Hospital Bremen and Children's Hospital of the University of Würzburg, Germany.
Panelius
Jaana
J
Hospital for Children and Adolescents, University of Helsinki, Stenbäckinkatu 11, 00290 Helsinki, Finland 2Department of Bacteriology and Immunology, Haartman Institute, Haartmaninkatu 3, 00014 Helsinki, Finland 3,7Department of Otolaryngology3 and Laboratory Diagnostics7, Helsinki University Central Hospital, 00290 Helsinki, Finland 4Division of Rheumatology, Allergy and Immunology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA 5Department of Infectious Diseases, University Medical Center Ljubljana, Slovenia 6Professor-Hess-Children's Hospital Bremen and Children's Hospital of the University of Würzburg, Germany.
Saxen
Harri
H
Hospital for Children and Adolescents, University of Helsinki, Stenbäckinkatu 11, 00290 Helsinki, Finland 2Department of Bacteriology and Immunology, Haartman Institute, Haartmaninkatu 3, 00014 Helsinki, Finland 3,7Department of Otolaryngology3 and Laboratory Diagnostics7, Helsinki University Central Hospital, 00290 Helsinki, Finland 4Division of Rheumatology, Allergy and Immunology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA 5Department of Infectious Diseases, University Medical Center Ljubljana, Slovenia 6Professor-Hess-Children's Hospital Bremen and Children's Hospital of the University of Würzburg, Germany.
Heikkilä
Tero
T
Hospital for Children and Adolescents, University of Helsinki, Stenbäckinkatu 11, 00290 Helsinki, Finland 2Department of Bacteriology and Immunology, Haartman Institute, Haartmaninkatu 3, 00014 Helsinki, Finland 3,7Department of Otolaryngology3 and Laboratory Diagnostics7, Helsinki University Central Hospital, 00290 Helsinki, Finland 4Division of Rheumatology, Allergy and Immunology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA 5Department of Infectious Diseases, University Medical Center Ljubljana, Slovenia 6Professor-Hess-Children's Hospital Bremen and Children's Hospital of the University of Würzburg, Germany.
Sillanpää
Heidi
H
Hospital for Children and Adolescents, University of Helsinki, Stenbäckinkatu 11, 00290 Helsinki, Finland 2Department of Bacteriology and Immunology, Haartman Institute, Haartmaninkatu 3, 00014 Helsinki, Finland 3,7Department of Otolaryngology3 and Laboratory Diagnostics7, Helsinki University Central Hospital, 00290 Helsinki, Finland 4Division of Rheumatology, Allergy and Immunology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA 5Department of Infectious Diseases, University Medical Center Ljubljana, Slovenia 6Professor-Hess-Children's Hospital Bremen and Children's Hospital of the University of Würzburg, Germany.
Peltomaa
Miikka
M
Hospital for Children and Adolescents, University of Helsinki, Stenbäckinkatu 11, 00290 Helsinki, Finland 2Department of Bacteriology and Immunology, Haartman Institute, Haartmaninkatu 3, 00014 Helsinki, Finland 3,7Department of Otolaryngology3 and Laboratory Diagnostics7, Helsinki University Central Hospital, 00290 Helsinki, Finland 4Division of Rheumatology, Allergy and Immunology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA 5Department of Infectious Diseases, University Medical Center Ljubljana, Slovenia 6Professor-Hess-Children's Hospital Bremen and Children's Hospital of the University of Würzburg, Germany.
Arnez
Maja
M
Hospital for Children and Adolescents, University of Helsinki, Stenbäckinkatu 11, 00290 Helsinki, Finland 2Department of Bacteriology and Immunology, Haartman Institute, Haartmaninkatu 3, 00014 Helsinki, Finland 3,7Department of Otolaryngology3 and Laboratory Diagnostics7, Helsinki University Central Hospital, 00290 Helsinki, Finland 4Division of Rheumatology, Allergy and Immunology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA 5Department of Infectious Diseases, University Medical Center Ljubljana, Slovenia 6Professor-Hess-Children's Hospital Bremen and Children's Hospital of the University of Würzburg, Germany.
Huppertz
Hans-Iko
HI
Hospital for Children and Adolescents, University of Helsinki, Stenbäckinkatu 11, 00290 Helsinki, Finland 2Department of Bacteriology and Immunology, Haartman Institute, Haartmaninkatu 3, 00014 Helsinki, Finland 3,7Department of Otolaryngology3 and Laboratory Diagnostics7, Helsinki University Central Hospital, 00290 Helsinki, Finland 4Division of Rheumatology, Allergy and Immunology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA 5Department of Infectious Diseases, University Medical Center Ljubljana, Slovenia 6Professor-Hess-Children's Hospital Bremen and Children's Hospital of the University of Würzburg, Germany.
Seppälä
Ilkka J T
IJT
Hospital for Children and Adolescents, University of Helsinki, Stenbäckinkatu 11, 00290 Helsinki, Finland 2Department of Bacteriology and Immunology, Haartman Institute, Haartmaninkatu 3, 00014 Helsinki, Finland 3,7Department of Otolaryngology3 and Laboratory Diagnostics7, Helsinki University Central Hospital, 00290 Helsinki, Finland 4Division of Rheumatology, Allergy and Immunology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA 5Department of Infectious Diseases, University Medical Center Ljubljana, Slovenia 6Professor-Hess-Children's Hospital Bremen and Children's Hospital of the University of Würzburg, Germany.
D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D025065:Borrelia burgdorferi / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D015787:Erythema Chronicum Migrans / Q000175:diagnosis* / Q000276:immunology; D005260:Female; D006801:Humans; D008297:Male; D011994:Recombinant Proteins / Q000276:immunology; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D011994:Recombinant Proteins; C106209:p35 antigen, Borrelia