Correlation of body temperature with protection against staphylococcal enterotoxin B exposure and use in determining vaccine dose-schedule.
The immunoprotective potential of a recombinant vaccine against the incapacitating effect of aerosolized staphylococcal enterotoxin B (SEB) in nonhuman primates is reported. SEB belongs to a family of structurally related superantigens responsible for serious, life threatening pathologies. Injecting the recombinant SEB vaccine did not induce temperature elevation in rhesus monkeys, a classical symptom of toxic-shock syndrome. No temperature elevation was noted following injection with control tetanus toxoid. In addition to 100% survival, we observed a clear correlation between vaccine dose and mitigation of temperature elevation after a lethal SEB aerosol challenge. We conclude that the recombinant SEB vaccine is non-pyrogenic and that monitoring changes in body temperature is an important biomarker of toxic shock in a primate animal model.
Boles
James W
JW
United States Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702, USA.
Pitt
M Louise M
ML
LeClaire
Ross D
RD
Gibbs
Paul H
PH
Ulrich
Robert G
RG
Bavari
Sina
S
D000818:Animals; D001831:Body Temperature / Q000276:immunology*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004768:Enterotoxins / Q000276:immunology*; D005334:Fever / Q000139:chemically induced; D006801:Humans; D007115:Immunization Schedule; D007117:Immunization, Secondary; D008253:Macaca mulatta; D013209:Staphylococcal Vaccines / Q000008:administration & dosage / Q000009:adverse effects / Q000276:immunology*; D013211:Staphylococcus aureus / Q000276:immunology; D018089:Superantigens / Q000276:immunology*; D013686:Telemetry; D013997:Time Factors; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000009:adverse effects / Q000276:immunology
D004768:Enterotoxins; D013209:Staphylococcal Vaccines; D018089:Superantigens; D014614:Vaccines, Synthetic; C031818:enterotoxin B, staphylococcal