Purification of gastric mucosal ECL cells from a crude elutriation fraction.
Acid-secreting parietal cells from the gastric mucosa are widely studied as a model in studies on ion transport and the endocrine/paracrine ECL cells effectively control parietal cell function. Discontinuous gradients of iodixanol for the purification of ECL cells were subsequently simplified to the use of a density barrier. This technique is now commonly used following initial centrifugal elutriation.
Graham
John M
JM
School of Biomolecular Sciences, Liverpool John Moores University. john@jgrescon.fsbusiness.co.uk
D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient / Q000379:methods; D019861:Enterochromaffin-like Cells / Q000378:metabolism*; D005753:Gastric Mucosa / Q000737:chemistry* / Q000166:cytology*; D014020:Tissue Extracts / Q000737:chemistry / Q000302:isolation & purification; D014283:Triiodobenzoic Acids / Q000737:chemistry
D014020:Tissue Extracts; D014283:Triiodobenzoic Acids; C044834:iodixanol