Math1 gene transfer generates new cochlear hair cells in mature guinea pigs in vivo.
Hair cell loss in the mammalian cochlea is irreversible and results in permanent hearing loss. Math1, the basic helix-loop-helix transcription factor homolog of the Drosophila atonal gene, is a positive regulator of hair cell differentiation during cochlear development. Developing hair cells express Math1, and nonsensory cells do not. We set out to determine the outcome of overexpression of Math1 in nonsensory cells of the cochlea on the phenotype of these cells. We demonstrate that in vivo inoculation of adenovirus with the Math1 gene insert into the endolymph of the mature guinea pig cochlea results in Math1 overexpression in nonsensory cochlear cells, as evident from the presence of Math1 protein in supporting cells of the organ of Corti and in adjacent nonsensory epithelial cells. Math1 overexpression leads to the appearance of immature hair cells in the organ of Corti and new hair cells adjacent to the organ of Corti in the interdental cell, inner sulcus, and Hensen cell regions. Axons are extended from the bundle of auditory nerve toward some of the new hair cells, suggesting that the new cells attract auditory neurons. We conclude that nonsensory cells in the mature cochlea retain the competence to generate new hair cells after overexpression of Math1 in vivo and that Math1 is necessary and sufficient to direct hair cell differentiation in these mature nonsensory cells.
Kawamoto
Kohei
K
Kresge Hearing Research Institute, Department of Otolaryngology, The University of Michigan, Ann Arbor, Michigan 48109-0648, USA.
Ishimoto
Shin-Ichi
S
Minoda
Ryosei
R
Brough
Douglas E
DE
Raphael
Yehoash
Y
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002467:Cell Nucleus / Q000378:metabolism; D003051:Cochlea / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D004398:Dyneins; D015870:Gene Expression; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000008:administration & dosage / Q000235:genetics; D006168:Guinea Pigs; D006198:Hair Cells, Auditory / Q000166:cytology*; D007267:Injections; D000081412:Myosin VIIa; D009218:Myosins / Q000096:biosynthesis; D009474:Neurons / Q000166:cytology; D012038:Regeneration / Q000187:drug effects / Q000502:physiology*; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D019076:Transgenes
D051792:Basic Helix-Loop-Helix Proteins; D004398:Dyneins; D000081412:Myosin VIIa; D009218:Myosins; D014157:Transcription Factors; C500943:Atoh1 protein, mouse; C000631456:Myo7a protein, mouse; C000631458:myosin 7a, Drosophila