Differentiation of CD34+ cells from human cord blood and murine bone marrow is suppressed by C6 beta-chemokines.
Several recently identified chemokines, Lkn-1, CKbeta8-1, MRP-2, and Mu C10 (MRP-1), are classified as C6 beta-chemokines. All of these chemokines have been found to suppress colony formation by bone marrow (BM) myeloid progenitors. Since cord blood (CB), like BM, contains CD34-positive cells, we examined the effects of these chemokines on CD34+ cells isolated from human CB. Lkn-1 and CKbeta8-1 suppressed colony formation by multi-potential granulocyte erythroid mega-karyocyte macrophages (CFU-GEMM), granulocyte-macrophages (CFU-GM), and erythroid (BFU-E) cells among the CD34+ cells from CB. CC chemokine receptor 1 (CCR1) that is known to be a receptor for Lkn-1 and CKbeta8-1 in neutrophils, monocytes, and lymphocytes, was also present on the surface of CD34+ cells from CB. Taken together these results suggest that Lkn-1 and CKbeta8-1 are active in inhibiting myeloid progenitor cells from both BM and CB. Macrophage inflammatory protein related protein-2 (mMRP-2) and Mu C10 (mMRP-1), which are murine C6 beta-chemokines, also inhibited colony formation by CB CD34+ cells. The inhibitory activity of these chemokines suggests that they may protect hematopoietic progenitors from the cytotoxic effects of the antiblastic drugs used in cancer therapy.
Han
In-Seob
IS
The Immunomodulation Research Center and Department of Biological Sciences, University of Ulsan, Ulsan 680- 748, Korea.
Ra
Jae-Sun
JS
Kim
Myung-Won
MW
Lee
Eun-A
EA
Jun
Hee-Young
HY
Park
Sang-Kyu
SK
Kwon
Byoung S
BS
D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D001853:Bone Marrow / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology*; D019742:Chemokines, CC / Q000378:metabolism*; D005312:Fetal Blood / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D019402:Macrophage Inflammatory Proteins / Q000378:metabolism; D051379:Mice; D000089762:Multidrug Resistance-Associated Protein 2
C000715087:ABCC2 protein, human; D018952:Antigens, CD34; C105504:CCL23 protein, human; C095218:Ccl9 protein, mouse; D019742:Chemokines, CC; D019402:Macrophage Inflammatory Proteins; D000089762:Multidrug Resistance-Associated Protein 2; C070959:Ccl6 protein, mouse