On the origins of cyanuric acid hydrolase: purification, substrates, and prevalence of AtzD from Pseudomonas sp. strain ADP.
Cyanuric acid hydrolase (AtzD) from Pseudomonas sp. strain ADP was purified to homogeneity. Of 22 cyclic amides and triazine compounds tested, only cyanuric acid and N-methylisocyanuric acid were substrates. Other cyclic amidases were found not to hydrolyze cyanuric acid. Ten bacteria that use cyanuric acid as a sole nitrogen source for growth were found to contain either atzD or trzD, but not both genes.
Fruchey
Isaac
I
Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, St. Paul, Minnesota 55108, USA.
Shapir
Nir
N
Sadowsky
Michael J
MJ
Wackett
Lawrence P
LP
D000581:Amidohydrolases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D019143:Evolution, Molecular; D006090:Gram-Negative Bacteria / Q000201:enzymology / Q000235:genetics; D006540:Herbicides / Q000378:metabolism; D011549:Pseudomonas / Q000201:enzymology* / Q000235:genetics*; D013379:Substrate Specificity; D014227:Triazines / Q000737:chemistry / Q000378:metabolism
D001426:Bacterial Proteins; D006540:Herbicides; D014227:Triazines; D000581:Amidohydrolases; C067141:cyanuric acid amidohydrolase; C004632:cyanuric acid