Protective action on human LDL against oxidation and glycation by four organosulfur compounds derived from garlic.
Human LDL were used to study the protective action of four organosulfur compounds (diallyl sulfide, DAS; diallyl disulfide, DADS; S-ethylcysteine, SEC; N-acetylcysteine, NAC) derived from garlic against oxidation and glycation. The four organosulfur compounds significantly inhibited superoxide production by xanthine-xanthine oxidase (P < 0.05) and showed marked copper-chelating capability. DAS and DADS exhibited greater antioxidant activities against copper- and amphotericin B-induced LDL oxidation (P < 0.05) than SEC and NAC. However, SEC and NAC were more effective in sparing LDL alpha-tocopherol (P < 0.05). When oxidation was minimized, SEC was the most powerful agent against LDL glycation (P < 0.05); however, DADS was superior to other agents in suppressing both oxidation and glycation when LDL oxidation occurred simultaneously with glycation. These results suggest that the four organosulfur compounds derived from garlic are potent agents for protecting LDL against oxidation and glycation, and that they may benefit patients with diabetes mellitus or cardiovascular diseases by preventing complications.
Ou
Chu-Chyn
CC
Department of Nutritional Science, Chungshan Medical University, Taichung City, Taiwan, ROC.
Tsao
Shyh-Ming
SM
Lin
Ming-Cheng
MC
Yin
Mei-Chin
MC
D000111:Acetylcysteine / Q000494:pharmacology; D000498:Allyl Compounds / Q000494:pharmacology; D000666:Amphotericin B / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology*; D002614:Chelating Agents / Q000494:pharmacology; D003300:Copper / Q000494:pharmacology; D003545:Cysteine / Q000031:analogs & derivatives* / Q000494:pharmacology; D004220:Disulfides / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D005737:Garlic; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D008077:Lipoproteins, LDL / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D013440:Sulfides / Q000494:pharmacology; D013457:Sulfur Compounds / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism; D014969:Xanthine Oxidase / Q000187:drug effects / Q000378:metabolism
D000498:Allyl Compounds; D000975:Antioxidants; D002614:Chelating Agents; D004220:Disulfides; D008077:Lipoproteins, LDL; D013440:Sulfides; D013457:Sulfur Compounds; D013481:Superoxides; C028009:diallyl disulfide; C038491:allyl sulfide; D003300:Copper; D000666:Amphotericin B; D004492:Edetic Acid; C026826:S-ethylcysteine; D014969:Xanthine Oxidase; D005947:Glucose; D003545:Cysteine; D000111:Acetylcysteine