Naphthalene biodegradation from non-aqueous-phase liquids in batch and column systems: comparison of biokinetic rate coefficients.
The kinetics of microbial degradation of naphthalene from a two-component non-aqueous-phase liquid (NAPL) coated onto uniformly sized nonporous particles were evaluated in a completely mixed batch reactor (CMBR) system and in flow-through column systems to examine the differences in the biodegradation kinetic coefficients, micro(max), the maximum specific growth rate coefficient, and K(s), the half saturation constant in the two systems. The values of these coefficients were estimated by nonlinear least-squares regression of the naphthalene mineralization profiles obtained from both CMBR and column biodegradation experiments. The results show that the range of values for micro(max) and K(s) obtained from column systems are very similar to the range of values obtained from CMBR systems. This suggests that coefficients estimated from CMBR or column systems are equally applicable for modeling studies. The presence of microorganisms and the development of biofilms at the NAPL-water interface reduced the mass transfer rates of naphthalene from the NAPL by 60% in CMBR and by 70% in column systems. If such changes in mass transfer coefficients are not accounted for, significantly erroneous values of biokinetic coefficients may be obtained.
Alshafie
Mohammed
M
Department of Civil Engineering and Applied Mechanics, McGill University, Montreal, Canada.
Ghoshal
Subhasis
S
D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000145:classification / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods*; D003102:Colloids; D003198:Computer Simulation; D007700:Kinetics; D008954:Models, Biological; D009281:Naphthalenes / Q000378:metabolism*; D044367:Phase Transition; D016958:Pseudomonas putida / Q000254:growth & development / Q000378:metabolism*; D014867:Water / Q000378:metabolism
D003102:Colloids; D009281:Naphthalenes; D014867:Water; C031721:naphthalene