title: Influence of the shielding on the induction of chromosomal aberrations in human lymphocytes exposed to high-energy iron ions.
abstract: Computer code calculations based on biophysical models are commonly used to evaluate the effectiveness of shielding in reducing the biological damage caused by cosmic radiation in space flights. Biological measurements are urgently needed to benchmark the codes. We have measured the induction of chromosomal aberrations in human peripheral blood lymphocytes exposed in vitro to 56Fe-ion beams accelerated at the HIMAC synchrotron in Chiba. Isolated lymphocytes were exposed to the 500 MeV/n iron beam (dose range 0.1-1 Gy) after traversal of 0 to 8 g/cm2 of either PMMA (lucite, a common plastic material) or aluminum. Three PMMA shield thickness and one Al shield thickness were used. For comparison, cells were exposed to 200 MeV/n iron ions and to X-rays. Chromosomes were prematurely condensed by a phosphatase inhibitor (calyculin A) to avoid cell-cycle selection produced by the exposure to high-LET heavy ion beams. Aberrations were scored in chromosomes 1, 2, and 4 following fluorescence in situ hybridization. The yield of chromosomal aberrations per unit dose at the sample position was poorly dependent on the shield thickness and material. However, the yield of aberrations per unit ion incident on the shield was increased by the shielding. This increase is associated to the increased dose-rate measured behind the shield as compared to the direct beam. These preliminary results prove that shielding can increase the effectiveness of heavy ions, and the damage is dependent upon shield thickness and material.
authors.affiliation: Dipartimento di Scienze Fisiche, Università Federico II, Monte S. Angelo, Via Cintia, 80126 Napoli, Italy. durante@na.infn.it
authors.forename: Marco
authors.initials: M
authors.lastname: Durante
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Gialanella
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Grossi
authors.forename: Mariagabriella
authors.initials: M
authors.lastname: Pugliese
authors.forename: Paola
authors.initials: P
authors.lastname: Scampoli
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Kawata
authors.forename: Nakahiro
authors.initials: N
authors.lastname: Yasuda
authors.forename: Yoshiya
authors.initials: Y
authors.lastname: Furusawa
mesh_terms: D000328:Adult; D000535:Aluminum; D001773:Blood Cells / Q000528:radiation effects; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000528:radiation effects; D004867:Equipment Design; D020450:Heavy Ions; D006801:Humans; D007501:Iron; D008214:Lymphocytes / Q000528:radiation effects*; D008297:Male; D010315:Particle Accelerators; D019904:Polymethyl Methacrylate; D011835:Radiation Protection / Q000295:instrumentation
chemical_list: D019904:Polymethyl Methacrylate; D000535:Aluminum; D007501:Iron