Ruthenium complexes carrying a disialo complex-type oligosaccharide: enzymatic synthesis and its application to a luminescent probe to detect influenza viruses.
Tris-bipyridine ruthenium-complexes carrying a disialo complex-type oligosaccharide were prepared via a one-pot transglycosylation using endo-glycosidase (Endo M); they bind to type-A influenza viruses with excellent affinity (IC50 = 8.4 microM), and their luminescence intensity is strongly depressed by virus-binding.
Kojima
Shinji
S
Department of Molecular Design, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan.
Hasegawa
Teruaki
T
Yonemura
Takahiro
T
Sasaki
Ken
K
Yamamoto
Kenji
K
Makimura
Yutaka
Y
Takahashi
Tadanobu
T
Suzuki
Takashi
T
Suzuki
Yasuo
Y
Kobayashi
Kazukiyo
K
D004798:Enzymes / Q000737:chemistry*; D049449:Luminescence; D015335:Molecular Probes; D009844:Oligosaccharides / Q000737:chemistry*; D009975:Orthomyxoviridae / Q000302:isolation & purification*; D012428:Ruthenium / Q000737:chemistry*
D004798:Enzymes; D015335:Molecular Probes; D009844:Oligosaccharides; D012428:Ruthenium