title: Carbocysteine lysine salt monohydrate (SCMC-LYS) is a selective scavenger of reactive oxygen intermediates (ROIs).
abstract: Carbocysteine lysine salt monohydrate (SCMC-Lys) is a well-known mucoactive drug whose therapeutic efficacy is commonly related to the ability of SCMC-Lys to replace fucomucins by sialomucins. The aim of this study was to determine if SCMC-Lys could exert an anti-oxidant action by scavenging reactive oxygen intermediates (ROIs). Our results show that SCMC-Lys proved effective as a selective scavenger of hypochlorous acid (HOCl) and hydroxyl radical (OH.), this effect being related to the reactivity of the SCMC tioether group. The scavenger activity of SCMC-Lys was observed in free cellular system as well as in activated human polymorphonuclear neutrophils (PMNs). SCMC-Lys scavenger activity on HOCl was paralleled by a powerful protection from HOCl-mediated inactivation of alpha1-antitripsin (alpha1-AT) inhibitor, the main serum protease inhibitor. Production of interleukin-(IL-)8, a major mediator of PMN recruitment in inflammatory diseases, is known to be mediated by intracellular OH. SCMC-Lys significantly reduced IL-8 production on stimulated human peripheral blood mononuclear cells (PBMCs) in the same range of concentrations affecting OH. activity. It is concluded that SCMC-Lys could exert, in addition to its mucoactive capacity, an anti-oxidant action, thus contributing to the therapeutic efficacy of SCMC-Lys.
authors.affiliation: Dompé SpA Research Center, via Campo di Pile, 67100 - L'Aquila, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Brandolini
authors.forename: Marcello
authors.initials: M
authors.lastname: Allegretti
authors.forename: Valerio
authors.initials: V
authors.lastname: Berdini
authors.forename: Maria Neve
authors.initials: MN
authors.lastname: Cervellera
authors.forename: Patrizia
authors.initials: P
authors.lastname: Mascagni
authors.forename: Matteo
authors.initials: M
authors.lastname: Rinaldi
authors.forename: Gabriella
authors.initials: G
authors.lastname: Melillo
authors.forename: Pietro
authors.initials: P
authors.lastname: Ghezzi
authors.forename: Manuela
authors.initials: M
authors.lastname: Mengozzi
authors.forename: Riccardo
authors.initials: R
authors.lastname: Bertini
mesh_terms: D002233:Carbocysteine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors*; D017665:Hydroxyl Radical / Q000037:antagonists & inhibitors; D006997:Hypochlorous Acid / Q000494:pharmacology*; D016209:Interleukin-8 / Q000096:biosynthesis / Q000097:blood; D007700:Kinetics; D008214:Lymphocytes / Q000187:drug effects / Q000502:physiology*; D030421:Peroxynitrous Acid / Q000037:antagonists & inhibitors; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors*
chemical_list: D016166:Free Radical Scavengers; D016209:Interleukin-8; D017382:Reactive Oxygen Species; D030421:Peroxynitrous Acid; C034932:carbocysteine-lysine; D017665:Hydroxyl Radical; D006997:Hypochlorous Acid; D002233:Carbocysteine; D006861:Hydrogen Peroxide