Isolation and determination of p-hydroxybenzoylcholine in traditional Chinese medicine Semen sinapis Albae.
A special component is isolated from Semen sinapis Albae (white mustard seed), a traditional Chinese medicine. According to the physical and chemical investigation and spectroscopic identification, this component can be known as p-hydroxybenzoylcholine bisulfate, a choline base. This component in the drug is also determined by RP-HPLC. A reversed-phase C(18) column is used to separate the p-hydroxybenzoylcholine with an eluent of methanol-0.05 mol/L monopotassium phosphate solution (30:70) (adjusted by phosphoric acid to pH 3.6) at the flow rate of 0.5 mL/min. Detection is carried out with a UV detector operated at 285 nm, and the column temperature is 25 degrees C. It reveals that there is 0.021% (w/w) of p-hydroxybenzoylcholine bisulfate in Semen sinapis Albae and 0.037% (w/w) in stir-baked Semen sinapis Albae.
Liu
Lifang
L
Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, 210093, Nanjing, P. R. China.
Liu
Tao
T
Li
Genxi
G
Wang
Qiang
Q
Ng
Tzibun
T
D001588:Benzoylcholine / Q000031:analogs & derivatives / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure; D012680:Sensitivity and Specificity; D031226:Sinapis / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D001588:Benzoylcholine; C039127:4-hydroxybenzoylcholine