Structure-dependent efficacy of infectious bursal disease virus (IBDV) recombinant vaccines.
The immunogenicity and protective capability of several baculovirus-expressed infectious bursal disease virus (IBDV)-derived assemblies as VP2 capsids, VPX tubules and polyprotein (PP)-derived mixed structures, were tested. Four-week-old chickens were immunised subcutaneously with one dose of each particulate antigen. VP2 icosahedral capsids induced the highest neutralising response, followed by PP-derived structures and then VPX tubules. All vaccinated animals were protected when challenged with a very virulent IBDV (vvIBDV) isolate, however the degree of protection is directly correlated with the levels of neutralising antibodies. VP2 capsids elicited stronger protective immunity than tubular structures and 3 micrograms of them were sufficient to confer a total protection comparable to that induced by an inactivated vaccine. Therefore, VP2 capsids represent a suitable candidate recombinant vaccine instead of virus-like particles (VLPs) for IBDV infections. Our results also provide clear evidence that the recombinant IBDV-derived antigens are structure-dependent in order to be efficient as vaccine components.
Martinez-Torrecuadrada
Jorge L
JL
INGENASA, Hermanos Garci;a Noblejas 41, 28037, Madrid, Spain. jlmartinez@cnio.es
Saubi
Narciís
N
Pagès-Manté
Albert
A
Castón
José R
JR
Espuña
Enric
E
Casal
J Ignacio
JI
D000818:Animals; D018175:Birnaviridae Infections / Q000276:immunology / Q000517:prevention & control; D002213:Capsid / Q000276:immunology; D002478:Cells, Cultured; D002645:Chickens / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D007114:Immunization; D007243:Infectious bursal disease virus / Q000737:chemistry / Q000276:immunology*; D011786:Quality Control; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018411:Spodoptera; D013329:Structure-Activity Relationship; D014614:Vaccines, Synthetic / Q000737:chemistry / Q000276:immunology; D014764:Viral Proteins / Q000096:biosynthesis; D014765:Viral Vaccines / Q000737:chemistry / Q000276:immunology*
D014614:Vaccines, Synthetic; D014764:Viral Proteins; D014765:Viral Vaccines