title: Interaction of replicase components between Cucumber mosaic virus and Peanut stunt virus.
abstract: Cucumber mosaic virus (CMV) and Peanut stunt virus (PSV) each have genomes consisting of three single-stranded RNA molecules: RNA 1, 2 and 3. RNAs 1 and 2 encode the 1a and 2a proteins, respectively, which are necessary for replication of the viral genome. Although RNA 3 is exchangeable between CMV and PSV, exchange of RNA 1 and 2 between the two viruses has been unsuccessful. In this study, reassortants containing PSV RNA 1 and CMV RNA 2 together with RNA 3 of CMV or PSV were shown to be able to replicate their genomic RNA, but not to transcribe subgenomic RNA 4 in tobacco protoplasts. Conversely, the reassortant consisting of CMV RNA 1 and PSV RNA 2 together with RNA 3 of CMV or PSV could not replicate. Subsequently, a yeast two-hybrid system was used to analyse the in vivo interaction between the 1a and 2a proteins. The C-terminal half of PSV-1a protein interacted with the N-terminal region of 2a protein of both PSV and CMV, but the C-terminal half of CMV-1a and the N-terminal region of PSV-2a did not interact. These results suggest that RNA replication in the interspecific reassortant between CMV and PSV requires compatibility between the C-terminal half of the 1a protein and the N-terminal region of the 2a protein, and this compatibility is insufficient for transcription of subgenomic RNA 4.
authors.affiliation: Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Suzuki
authors.affiliation: Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Megumi
authors.initials: M
authors.lastname: Yoshida
authors.affiliation: Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Yoshinuma
authors.affiliation: Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Tadaaki
authors.initials: T
authors.lastname: Hibi
mesh_terms: D010367:Arachis / Q000821:virology*; D018553:Cucumis sativus / Q000821:virology*; D017799:Cucumovirus / Q000201:enzymology* / Q000235:genetics; D011523:Protoplasts / Q000821:virology; D012367:RNA, Viral / Q000096:biosynthesis; D012324:RNA-Dependent RNA Polymerase / Q000737:chemistry / Q000378:metabolism*; D011995:Recombination, Genetic; D020798:Two-Hybrid System Techniques; D014779:Virus Replication
chemical_list: D012367:RNA, Viral; D012324:RNA-Dependent RNA Polymerase