title: Evaluation of a new pretreatment reagent for use with the EMIT 2000 cyclosporine assay.
abstract: OBJECTIVE
  Evaluate the performance of the new pretreatment (NPT) reagent for use with the EMIT cyclosporine A (CsA) assay.
  
  
  METHODS
  Samples from transplant patients receiving CsA were tested using a COBAS MIRA S.
  
  
  RESULTS
  A downward shift in target values for commercial controls was observed using the NPT reagent. There was excellent correlation for patient results when comparing the NPT reagent with methanol pretreatment.
  
  
  CONCLUSIONS
  The NPT reagent is an acceptable substitute for methanol in the EMIT(R) extraction procedure for CsA.
authors.affiliation: Department of Pathology & Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095-1713, USA. abutch@mednet.ucla.edu
authors.forename: Anthony W
authors.initials: AW
authors.lastname: Butch
authors.forename: Alan M
authors.initials: AM
authors.lastname: Fukuchi
mesh_terms: D016572:Cyclosporine / Q000097:blood*; D017248:Enzyme Multiplied Immunoassay Technique / Q000295:instrumentation / Q000592:standards*; D006801:Humans; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D015203:Reproducibility of Results
chemical_list: D011933:Reagent Kits, Diagnostic; D016572:Cyclosporine