Extracellular matrix as a scaffold for laryngeal reconstruction.
Porcine-derived xenogeneic extracellular matrix (ECM) has been successfully used as a scaffold for tissue repair and reconstruction in numerous preclinical animal studies and human applications. These scaffolds are completely and rapidly degraded and replaced by host-derived tissues that frequently mimic the original tissue composition and architecture. The purpose of the present study was to examine the morphology of ECM scaffolds after their use for laryngeal reconstruction. Thirty adult female dogs were subjected to a partial hemilaryngectomy. The right thyroid cartilage and vocal fold were replaced with ECM scaffold, and the dogs were painlessly sacrificed from 1 week to 12 months after surgery. Histologic examination of the reconstructed tissue showed the presence of a simple squamous epithelial lining, organized glandular structures within the submucosa, reconstructed thyroid cartilage, and bundles of skeletal muscle by 3 months after surgery. We conclude that ECM scaffolds are promising templates for constructive remodeling of laryngeal tissue.
Huber
Jessica E
JE
Department of Audiology and Speech Sciences, Purdue University, West Lafayette, Indiana 47907-2038, USA.
Spievack
Alan
A
Simmons-Byrd
Abby
A
Ringel
Robert L
RL
Badylak
Stephen
S
D000818:Animals; D004285:Dogs; D005109:Extracellular Matrix / Q000637:transplantation* / Q000648:ultrastructure; D005260:Female; D007825:Laryngectomy / Q000379:methods; D007830:Larynx / Q000473:pathology; D023421:Models, Animal; D012038:Regeneration / Q000502:physiology*; D013552:Swine; D013957:Thyroid Cartilage / Q000502:physiology* / Q000601:surgery; D023822:Tissue Engineering / Q000379:methods*; D014183:Transplantation, Heterologous; D014827:Vocal Cords / Q000502:physiology* / Q000601:surgery; D014945:Wound Healing / Q000502:physiology