[Relationship between aquaporin-4 expression in astrocytes and brain edema caused by glioma].
OBJECTIVE
To investigate the relationship between aquaporin-4 (AQP4) water channel expression in astrocytes and brain edema caused by glioma.


METHODS
The changes of water transport in in vitro blood-brain barrier models were examined using high-performance liquid chromatography (HPLC), and the expression level of AQP4 was assayed by semiquantitative RT-PCR.


RESULTS
The water transport of the in vitro blood-brain barrier model from the luminal side to basolateral side was increased after coculture with glioma cells, which induced significantly decreased expression level of AQP4 in the astrocytes.


CONCLUSIONS
Glioma cells can promote water transport in in vitro blood-brain barrier model from the luminal side to abluminal side, and the brain edema induced by the glioma cells may not necessarily be the result of hyperpermeability of the blood-brain barrier to macromolecules in the plasma. The decreased expression level of AQP4 induced by glioma cells may be a molecular mechanism for neoplastic brain edema in patients with glioma.
Chen
Yi-zhao
YZ
Department of Neurosurgery, First Military Medical University, Guangzhou 510282, China. chenyizhao@21cn.com
Xu
Ru-xiang
RX
Xu
Zong-jun
ZJ
Yang
Zhi-lin
ZL
Jiang
Xiao-dan
XD
Cai
Ying-qian
YQ
D000818:Animals; D051401:Aquaporin 4; D020346:Aquaporins / Q000235:genetics*; D001253:Astrocytes / Q000378:metabolism*; D001812:Blood-Brain Barrier; D001834:Body Water / Q000378:metabolism; D001929:Brain Edema / Q000209:etiology*; D005910:Glioma / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
C496032:Aqp4 protein, rat; D051401:Aquaporin 4; D020346:Aquaporins