Effect of hybrid complement regulatory proteins on xenogeneic cells.
To suppress C3 fragment deposition in the classical pathway complement activation on xenogeneic membranes, decay accelerating factor (DAF) was the most effective molecule among the complement regulatory proteins (CRPs) used in the present study. C3 fragment deposition was closely related to subsequent xenogeneic cell lysis. However, other molecules were also very effective in different ways and include phosphatidylinositol (PI)-anchored short consensus repeat (SCR) 2-4 of membrane cofactor protein (MCP-PI), PI-anchored C1 esterase inhibitor (C1-INH-PI), and PI-anchored SCR8-11 of complement receptor type 1 (CR1-PI). On the other hand, regarding a strategy for downregulating C4 fragment deposition, the use of only C1-INH-PI and PI-anchored SCR1-3 of the C4b-binding protein (C4bp-PI) was found to be effective.
Fukuta
Daisuke
D
Department of Regenerative Medicine, Division of Organ Transplantation, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, 565-0871, Shiga, Japan.
Miyagawa
Shuji
S
Kubo
Tomoko
T
Matsunami
Katsuyoshi
K
Shirasu
Akio
A
Hattori
Hiroyuki
H
Shirakura
Ryota
R
D000818:Animals; D016466:CHO Cells; D002469:Cell Separation; D003176:Complement C3 / Q000378:metabolism; D003181:Complement C4 / Q000378:metabolism*; D003165:Complement System Proteins; D006224:Cricetinae; D018076:DNA, Complementary / Q000378:metabolism; D015536:Down-Regulation; D005434:Flow Cytometry; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D014162:Transfection
D003176:Complement C3; D003181:Complement C4; D018076:DNA, Complementary; D010716:Phosphatidylinositols; D003165:Complement System Proteins; D007770:L-Lactate Dehydrogenase