title: Apoptotic DNA endonuclease (DNase-gamma) gene transfer induces cell death accompanying DNA fragmentation in human glioma cells.
abstract: OBJECTIVE
  Both the genetic restoration of the apoptotic pathway and the introduction of proapoptotic molecules are now drawing attention. Concerning apoptosis of human glioma cells induced by human interferon-beta protein, we found that DNA endonuclease (DNase-gamma) acts as an executive molecule. The authors investigated whether gene transfer of this DNase-gamma exerts some therapeutic effects on human glioma cells.
  
  
  METHODS
  We transduced U251SP, U251MG, and T98G human glioma cells with DNase-gamma gene via multilamellar cationic liposomes, monitored the growth of those cells, and carefully observed the cell-death pattern.
  
  
  RESULTS
  DNase-gamma gene transfer resulted in an overexpression of DNase-gamma protein and induced DNA fragmentation in gene-transferred cells. The cytotoxic effect rose with multiple inoculations of the liposome, suggesting a relationship between its expression and the therapeutic effect.
  
  
  CONCLUSIONS
  These results demonstrate that DNase-gamma gene transfer can induce apoptosis in human glioma cells, indicating its potential to become a future gene therapy strategy.
authors.affiliation: Department of Neurosurgery, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Ryuta
authors.initials: R
authors.lastname: Saito
authors.forename: Masaaki
authors.initials: M
authors.lastname: Mizuno
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Kumabe
authors.forename: Takashi
authors.initials: T
authors.lastname: Yoshimoto
authors.forename: Sei-ichi
authors.initials: S
authors.lastname: Tanuma
authors.forename: Jun
authors.initials: J
authors.lastname: Yoshida
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000201:enzymology* / Q000473:pathology*; D002455:Cell Division; D004273:DNA, Neoplasm / Q000032:analysis; D004706:Endodeoxyribonucleases / Q000235:genetics*; D018014:Gene Transfer Techniques; D005910:Glioma / Q000201:enzymology* / Q000473:pathology*; D006801:Humans; D008081:Liposomes; D010957:Plasmids; D014407:Tumor Cells, Cultured
chemical_list: D004273:DNA, Neoplasm; D008081:Liposomes; D004706:Endodeoxyribonucleases; C105124:deoxyribonuclease gamma