Circulating recombinant form CRF02_AG in South America.
With the objective of monitoring the distribution of HIV-1 subtypes and circulating recombinant forms (CRFs)in South America, population-based surveillance studies were performed in seven countries. Peripheral blood mononuclear cell, filter paper, fresh blood, and cocultivation samples were collected from HIV-positive patients from Colombia, Ecuador, Peru, Bolivia, Chile, Argentina, and Uruguay, during a 7-year period(1995-2001). DNA was prepared and HIV envelope subtypes were determined by heteroduplex mobility as-say and DNA sequencing from 1289 HIV-positive samples. While subtypes B and F were the most commonly observed subtypes, two CRF02_AG strains were detected, in Ecuador. This is the first report of the existence of this CRF in South America.
Carrion
G
G
US Naval Medical Research Center Detachment, Lima, Peru.
Hierholzer
J
J
Montano
S
S
Alava
A
A
Perez
J
J
Guevara
A
A
Laguna-Torres
V
V
Mosquera
C
C
Russell
K
K
Chauca
G
G
Kochel
T
T
Birx
D L
DL
Sanchez
J L
JL
Carr
J K
JK
D000328:Adult; D004279:DNA, Viral / Q000097:blood; D004484:Ecuador / Q000453:epidemiology; D005260:Female; D015658:HIV Infections / Q000453:epidemiology* / Q000821:virology; D015497:HIV-1 / Q000145:classification* / Q000235:genetics; D020180:Heteroduplex Analysis; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D011159:Population Surveillance; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D013020:South America / Q000453:epidemiology
D004279:DNA, Viral