title: Helper T-cell type 1 or type 2 function of xeno-MHC-restricted T-cell clones in a direct xenoantigen recognition.
abstract: There have been several reports that xeno-MHC-restricted T-cells have a cytotoxic function through a direct xenoantigen recognition, but yet no report that they have a helper function. Previously we showed that both xeno-MHC-restricted CD4(+) and CD8(+) T-cells recognized xenoantigens directly in a mouse anti-rat combination. In this study, we investigated whether or not xeno-MHC-restricted T-cells had a helper function. Mouse T-cell clones recognizing rat antigens directly were derived from T-cell lines using the limiting dilution method. Phenotype, cytotoxic activity and cytokine production of these clones were analyzed by flow cytometry, 51Cr release assay and ELISA, respectively. Rat-MHC class I-restricted mouse CD8(+) T-cell clones showed a specific cytotoxic activity against rat antigens. One CD4(+) clone produced IL-4 and IL-10, and the other CD4(+) clone produced not T-helper (Th) 2 cytokine but TNF-alpha. Our results suggested that xeno-MHC class I-restricted CD8(+) T-cells should have a cytotoxic function, and xeno-MHC class II-restricted CD4(+) T-cells should have either Th1 or Th2 function.
authors.affiliation: First Department of Surgery, Gifu University School of Medicine, 40 Tukasa-machi, Gifu City, 5008705, Gifu, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Iida
authors.forename: Hisashi
authors.initials: H
authors.lastname: Iwata
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Mori
authors.forename: Hisato
authors.initials: H
authors.lastname: Takagi
authors.forename: Toyoo
authors.initials: T
authors.lastname: Nitta
authors.forename: Kazuto
authors.initials: K
authors.lastname: Kanetake
authors.forename: Yukio
authors.initials: Y
authors.lastname: Umeda
authors.forename: Hajime
authors.initials: H
authors.lastname: Hirose
mesh_terms: D000818:Animals; D015478:Antigens, Heterophile / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D003602:Cytotoxicity, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D008285:Major Histocompatibility Complex / Q000276:immunology*; D051379:Mice; D010641:Phenotype; D051381:Rats; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
chemical_list: D015478:Antigens, Heterophile; D016207:Cytokines