title: [Purification of porcine pulmonary surfactant by CO2 supercritical fluid extraction].
abstract: OBJECTIVE
  To develop a supercritical fluid extraction (SFE) method for purifying porcine pulmonary surfactant.
  
  
  METHODS
  An orthogonal design with 4 factors and 4 levels was used to optimize the SFE conditions.
  
  
  RESULTS
  The best extraction conditions were established as the following: pressure 30 MPa, temperature 50 degrees Celsius, dynamic extraction for 4 h with 0.2 ml ethanol as the modifier.
  
  
  CONCLUSIONS
  The new method for purifying porcine pulmonary surfactant by SFE-CO2 can cause drastic reduction of organic solvents, being convenient to use and effective to purify.
authors.affiliation: Department of Pediatrics, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhao
authors.forename: Zhi-chun
authors.initials: ZC
authors.lastname: Feng
authors.forename: Jiang
authors.initials: J
authors.lastname: Du
mesh_terms: D000818:Animals; D002245:Carbon Dioxide; D010743:Phospholipids / Q000032:analysis; D011663:Pulmonary Surfactants / Q000032:analysis / Q000302:isolation & purification*; D013552:Swine; D013696:Temperature
chemical_list: D010743:Phospholipids; D011663:Pulmonary Surfactants; D002245:Carbon Dioxide