[Differential expression of genes related to photoperiod-temperature sensitive genic male sterility in wheat, revealed by mRNA differential display using G-box family primer].
mRNA differential display with G-box family primer was used to analyze the differential expression of genes of the photoperiod-temperature sensitive genic male sterile(PTSGMS) line of wheat, BAU3338, between the sterile and fertile conditions. The result indicated that gene expression was significantly different between the two types of condition during the fertility transformation phase. The twelve qualitatively different DNA bands were identified with reverse Northern blot hybridization and five positive clones, HT1-G10, HT1-G3, HT2-G2, HT1-G4 and HT2-G5 were sequenced. The homology search indicated that HT1-G10 was highly homological (96%) to the partial sequences of Triticum aestivum chloroplast genes, rbcL and atpB, HT1-G3 was also homological (88%) to Triticum aestivum histone H2A gene and the other three gene fragments were new sequences in Gen-Bank. The analysis of the candidate gene fragments supplied some effective evidences to reveal the developmental mechanism of PTSGMS.
Cao
Shuang-He
SH
Institute of Genetics and Developmental Biology, CAS, Beijing 100101, China.
Liu
Dong-Cheng
DC
Liu
Li-Ke
LK
Guo
Xiao-Li
XL
Zhang
Ai-Min
AM
D015152:Blotting, Northern; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D005298:Fertility / Q000235:genetics; D051790:G-Box Binding Factors; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D017440:Photoperiod; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D013696:Temperature; D014157:Transcription Factors / Q000235:genetics*; D014908:Triticum / Q000235:genetics*
D017931:DNA Primers; D018076:DNA, Complementary; D018744:DNA, Plant; D004268:DNA-Binding Proteins; D051790:G-Box Binding Factors; D012333:RNA, Messenger; D014157:Transcription Factors