title: Circulating recombinant form CRF02_AG in South America.
abstract: With the objective of monitoring the distribution of HIV-1 subtypes and circulating recombinant forms (CRFs)in South America, population-based surveillance studies were performed in seven countries. Peripheral blood mononuclear cell, filter paper, fresh blood, and cocultivation samples were collected from HIV-positive patients from Colombia, Ecuador, Peru, Bolivia, Chile, Argentina, and Uruguay, during a 7-year period(1995-2001). DNA was prepared and HIV envelope subtypes were determined by heteroduplex mobility as-say and DNA sequencing from 1289 HIV-positive samples. While subtypes B and F were the most commonly observed subtypes, two CRF02_AG strains were detected, in Ecuador. This is the first report of the existence of this CRF in South America.
authors.affiliation: US Naval Medical Research Center Detachment, Lima, Peru.
authors.forename: G
authors.initials: G
authors.lastname: Carrion
authors.forename: J
authors.initials: J
authors.lastname: Hierholzer
authors.forename: S
authors.initials: S
authors.lastname: Montano
authors.forename: A
authors.initials: A
authors.lastname: Alava
authors.forename: J
authors.initials: J
authors.lastname: Perez
authors.forename: A
authors.initials: A
authors.lastname: Guevara
authors.forename: V
authors.initials: V
authors.lastname: Laguna-Torres
authors.forename: C
authors.initials: C
authors.lastname: Mosquera
authors.forename: K
authors.initials: K
authors.lastname: Russell
authors.forename: G
authors.initials: G
authors.lastname: Chauca
authors.forename: T
authors.initials: T
authors.lastname: Kochel
authors.forename: D L
authors.initials: DL
authors.lastname: Birx
authors.forename: J L
authors.initials: JL
authors.lastname: Sanchez
authors.forename: J K
authors.initials: JK
authors.lastname: Carr
mesh_terms: D000328:Adult; D004279:DNA, Viral / Q000097:blood; D004484:Ecuador / Q000453:epidemiology; D005260:Female; D015658:HIV Infections / Q000453:epidemiology* / Q000821:virology; D015497:HIV-1 / Q000145:classification* / Q000235:genetics; D020180:Heteroduplex Analysis; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D011159:Population Surveillance; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D013020:South America / Q000453:epidemiology
chemical_list: D004279:DNA, Viral