Comparison of washing and swabbing procedures for collecting genital fluids to assess cervicovaginal shedding of herpes simplex virus type 2 DNA.
Asymptomatic genital shedding of herpes simplex virus type 2 (HSV-2) DNA was evidenced by real-time PCR in 25 (13.2%) of 188 cervicovaginal lavage samples and in only 13 (6.9%) paired cervicovaginal samples from 188 HSV-2-seropositive, nonpregnant childbearing-aged human immunodeficiency virus-seronegative women living in Gabon. These observations demonstrate that cervicovaginal washing is more suitable than endocervicovaginal swabbing for detecting and quantifying HSV-2 DNA by PCR in female genital secretions.
Ndjoyi-Mbiguino
Angélique
A
Laboratoire de Virologie, Département de Microbiologie, Université des Sciences de la Santé Maternité Joséphine Bongo, Gabon.
Ozouaki
Francis
F
Legoff
Jérôme
J
Mbopi-Kéou
François-Xavier
FX
Si-Mohamed
Ali
A
Onas
Isabelle Ndombi
IN
Avoune
Evelyne
E
Bélec
Laurent
L
D000293:Adolescent; D000328:Adult; D001826:Body Fluids / Q000821:virology; D002584:Cervix Uteri / Q000821:virology; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006558:Herpes Genitalis / Q000821:virology*; D018258:Herpesvirus 2, Human / Q000302:isolation & purification*; D006801:Humans; D016133:Polymerase Chain Reaction; D013048:Specimen Handling / Q000379:methods*; D014621:Vagina / Q000821:virology; D017201:Virus Shedding
D004279:DNA, Viral