title: Identification and characterization of a novel large insertion/deletion polymorphism of 1464 base pair in the human thyroglobulin gene.
abstract: We identified a novel large insertion/deletion (Indel) polymorphism of 1464 bp localized in intron 18 of the human thyroglobulin gene. Data from sequence showed a high A+T content (62%), two 17-bp long motif repeats, and three different types of 10-bp long palindromic sequences. The comparison between these 1464 bp and sequences deposited in National Center for Biotechnology Information (NCBI)/GenBank database exhibit a nonsignificant degree of homology with any previously described sequences. The long polymerase chain reaction (PCR) method was used to amplify the genomic DNA region containing intron 17/exon 18/intron 18/exon 19/intron 19 by primers situated in the introns 17 and 19. The amplification generates two fragments of 3.5 and 5.0 kb that correspond to the exclusion or inclusion of a 1464-bp segment, respectively. Both variants are thus widely represented in the human population; giving allele frequencies of 0.56 (insertion) and 0.44 (deletion). Finally, the polymorphism was confirmed by sequence analysis of the 5.0- and 3.5-kb amplified fragments.
authors.affiliation: Laboratorio de Biología Molecular, Cátedra de Genética y Biología Molecular, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Christian M
authors.initials: CM
authors.lastname: Moya
authors.forename: Viviana
authors.initials: V
authors.lastname: Varela
authors.forename: Carina M
authors.initials: CM
authors.lastname: Rivolta
authors.forename: Fernando M
authors.initials: FM
authors.lastname: Mendive
authors.forename: Héctor M
authors.initials: HM
authors.lastname: Targovnik
mesh_terms: D000483:Alleles; D001483:Base Sequence / Q000235:genetics; D004251:DNA Transposable Elements; D017353:Gene Deletion; D005787:Gene Frequency; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D013954:Thyroglobulin / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D013954:Thyroglobulin