Expression of human Calbindin-D(9k) correlated with age, vitamin D receptor and blood calcium level in the gastrointestinal tissues.
OBJECTIVE
Calbindin-D(9k) (CaBP-9k) has been thought to be one of the important factors of calcium absorption and metabolism in the intestine. The understanding of CaBP-9k role in the gastrointestinal tissues is important in human in relation with aging and gender, and whether there is any relationship with mineral homeostasis and risk for the development of age-related calcium disorders such as osteoporosis.


METHODS
The expression of CaBP-9k and vitamin D receptor (VDR) was analyzed in the human gastrointestinal tissues with different ages and gender. In addition, the correlation among CaBP-9k expression, age and calcium concentration in blood was elucidated in these tissues.


RESULTS
A significant increase in the expression of CaBP-9k mRNA was observed in the bulb and 2(nd) portion of duodenum over age in both men and women. However, no significant age-associated change in VDR mRNA level was detected in the duodenum. The concentration of blood calcium level decreased with age in both men and women.


CONCLUSIONS
The duodenal CaBP-9k may be not involved in calcium absorption in these tissues. The functional significance of the increase in the intestinal CaBP-9k expression with age is not currently clear and requires further investigation.
Lee
Geun-Shik
GS
Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea.
Choi
Kyung-Chul
KC
Park
Seon-Mee
SM
An
Beum-Soo
BS
Cho
Myeong-Chan
MC
Jeung
Eui-Bae
EB
D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging; D015139:Blotting, Southern; D064026:Calbindins; D002118:Calcium / Q000097:blood*; D004064:Digestive System / Q000378:metabolism*; D004386:Duodenum / Q000378:metabolism; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D008297:Male; D008875:Middle Aged; D011706:Pyloric Antrum / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018167:Receptors, Calcitriol / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D064030:S100 Calcium Binding Protein G / Q000235:genetics*; D012737:Sex Factors
D064026:Calbindins; D012333:RNA, Messenger; D018167:Receptors, Calcitriol; D064030:S100 Calcium Binding Protein G; D002118:Calcium