title: Nuclear immunostaining in rat neuronal cells using two anti-Kir2.2 ion channel polyclonal antibodies.
abstract: The inwardly rectifying potassium ion channel Kir2.2 has recently been demonstrated to have nuclear and plasma membrane subcellular localization. Nuclear expression of Kir2.2 is controversial, as a functional role for Kir2.0 potassium channels in the nucleus has not been investigated. However, in this report we have demonstrated Kir2.2 nuclear localization in sections of rat hindbrain and dorsal root ganglia tissue, using two anti- Kir2.2 polyclonal antisera with different epitope specificities. These data confirm nuclear localization and are suggestive of new functions of Kir2.0 potassium ion channels in the nucleus.
authors.affiliation: The Neurosciences Institute, 10640 John Jay Hopkins Drive, San Diego, CA 92121, USA. stonehouse@nsi.edu
authors.forename: Anthony H
authors.initials: AH
authors.lastname: Stonehouse
authors.forename: Blair D
authors.initials: BD
authors.lastname: Grubb
authors.forename: J Howard
authors.initials: JH
authors.lastname: Pringle
authors.forename: Robert I
authors.initials: RI
authors.lastname: Norman
authors.forename: Peter R
authors.initials: PR
authors.lastname: Stanfield
authors.forename: William J
authors.initials: WJ
authors.lastname: Brammar
mesh_terms: D000818:Animals; D000906:Antibodies; D000918:Antibody Specificity / Q000276:immunology; D002451:Cell Compartmentation / Q000502:physiology; D002467:Cell Nucleus / Q000378:metabolism*; D000939:Epitopes / Q000276:immunology; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D024661:Potassium Channels, Inwardly Rectifying / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D012249:Rhombencephalon / Q000166:cytology / Q000378:metabolism*
chemical_list: D000906:Antibodies; D000939:Epitopes; D024661:Potassium Channels, Inwardly Rectifying