title: Synaptic changes characterize early behavioural signs in the ME7 model of murine prion disease.
abstract: Prion diseases are fatal, chronic neurodegenerative diseases of mammals, characterized by amyloid deposition, astrogliosis, microglial activation, tissue vacuolation and neuronal loss. In the ME7 model of prion disease in the C57BL/6 J mouse, we have shown previously that these animals display behavioural changes that indicate the onset of neuronal dysfunction. The current study examines the neuropathological correlates of these early behavioural changes. After injection of ME7-infected homogenate into the dorsal hippocampus, we found statistically significant impairment of burrowing, nesting and glucose consumption, and increased open field activity at 13 weeks. At this time, microglia activation and PrPSc deposition was visible selectively throughout the limbic system, including the hippocampus, entorhinal cortex, medial and lateral septum, mamillary bodies, dorsal thalamus and, to a lesser degree, in regions of the brainstem. No increase in apoptosis or neuronal cell loss was detectable at this time, while in animals at 19 weeks postinjection there was 40% neuronal loss from CA1. There was a statistically significant reduction in synaptophysin staining in the stratum radiatum of the CA1 at 13 weeks indicating loss of presynaptic terminals. Damage to the dorsal hippocampus is known to disrupt burrowing and nesting behaviour. We have demonstrated a neuropathological correlate of an early behavioural deficit in prion disease and suggest that this should allow insights into the first steps of the neuropathogenesis of prion diseases.
authors.affiliation: CNS Inflammation Group, Southampton Neuroscience Group, School of Biological Sciences, University of Southampton, Southampton SO16 7PX, UK. C.Cunningham@soton.ac.uk
authors.forename: C
authors.initials: C
authors.lastname: Cunningham
authors.forename: R
authors.initials: R
authors.lastname: Deacon
authors.forename: H
authors.initials: H
authors.lastname: Wells
authors.forename: D
authors.initials: D
authors.lastname: Boche
authors.forename: S
authors.initials: S
authors.lastname: Waters
authors.forename: C Picanco
authors.initials: CP
authors.lastname: Diniz
authors.forename: H
authors.initials: H
authors.lastname: Scott
authors.forename: J N P
authors.initials: JN
authors.lastname: Rawlins
authors.forename: V H
authors.initials: VH
authors.lastname: Perry
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000473:pathology; D001522:Behavior, Animal; D016923:Cell Death; D004435:Eating; D005260:Female; D005947:Glucose / Q000494:pharmacology; D006706:Homeostasis; D051379:Mice; D008810:Mice, Inbred C57BL; D017628:Microglia / Q000473:pathology; D009043:Motor Activity; D009410:Nerve Degeneration / Q000473:pathology*; D009425:Nesting Behavior; D009474:Neurons / Q000737:chemistry / Q000473:pathology; D018620:PrPSc Proteins / Q000032:analysis; D017096:Prion Diseases / Q000473:pathology*; D013569:Synapses / Q000473:pathology*; D016708:Synaptophysin / Q000032:analysis
chemical_list: D018620:PrPSc Proteins; D016708:Synaptophysin; D005947:Glucose