Isolation of a mouse motoneuron-enriched fraction from mouse spinal cord on a density barrier.
After a combined enzymic and mechanical disruption of the spinal cord tissue, the low-density motoneurons band at the interface of a 1.06-g/ml barrier through which other contaminating cells sediment.
Graham
John M
JM
School of Biomolecular Sciences, Liverpool John Moores University. john@jgrescon.fsbusiness.co.uk
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D002498:Centrifugation / Q000379:methods*; D051379:Mice; D009046:Motor Neurons / Q000166:cytology*; D013116:Spinal Cord / Q000166:cytology*; D014283:Triiodobenzoic Acids / Q000737:chemistry
D014283:Triiodobenzoic Acids; C044834:iodixanol