title: Estrogen modulates ClC-2 chloride channel gene expression in rat kidney.
abstract: ClC-2 is a CLC family member of chloride channels sensitive to changes in cell volume, pH and voltage. The ClC-2 is widely distributed along the nephron although in the kidney its role still not well understood. Aldosterone studies suggest that ClC-2 expression in the kidney may be hormonally regulated. To explore the possibility that estrogen control ClC-2 expression, we investigated whether its expression changed in the kidney of female Wistar rats subjected to ovariectomy with or without near-physiological or high doses of 17beta-estradiol benzoate treatment for 10 days. Total RNA isolated from rat kidney and dissected nephron segments was analyzed by ribonuclease protection assay and/or a semi-quantitative RT-PCR. The renal ClC-2 protein expression was analyzed by Western blot. The decreased renal expression of ClC-2 mRNA and protein observed in ovariectomized rats was restored to control levels after treatment with low doses of estradiol. Higher dose estradiol lead to an even greater increase in ClC-2 mRNA and protein expression. This change in overall expression was shown to be caused by the modulation of ClC-2 mRNA expression in the proximal tubule. These results suggest that ClC-2 may be involved in estrogen-induced Cl(-) transport in rat kidney.
authors.affiliation: Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, 21949-900 Rio de Janeiro, Brazil.
authors.forename: Danielle S
authors.initials: DS
authors.lastname: Nascimento
authors.forename: Carlos U
authors.initials: CU
authors.lastname: Reis
authors.forename: Regina C
authors.initials: RC
authors.lastname: Goldenberg
authors.forename: Tânia M
authors.initials: TM
authors.lastname: Ortiga-Carvalho
authors.forename: Carmen C
authors.initials: CC
authors.lastname: Pazos-Moura
authors.forename: Sandra E
authors.initials: SE
authors.lastname: Guggino
authors.forename: William B
authors.initials: WB
authors.lastname: Guggino
authors.forename: Marcelo M
authors.initials: MM
authors.lastname: Morales
mesh_terms: D000818:Animals; D015153:Blotting, Western; D000090007:CLC-2 Chloride Channels; D002478:Cells, Cultured; D018118:Chloride Channels / Q000235:genetics*; D004958:Estradiol / Q000097:blood / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D007687:Kidney Tubules, Proximal / Q000166:cytology / Q000502:physiology*; D009399:Nephrons / Q000502:physiology; D010052:Ovariectomy; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000090007:CLC-2 Chloride Channels; D018118:Chloride Channels; D012333:RNA, Messenger; D004958:Estradiol