title: Kinetics of CCR7 expression differ between primary activation and effector memory states of T(H)1 and T(H)2 cells.
abstract: We explored the kinetics of CCR7 expression on T(H)1 and T(H)2 polarized cells as well as on antigen-specific T cell lines at various stages of differentiation. A striking pattern of early (days 7-14) inducible CCR7 expression was seen preferentially on primary T(H)1 cell lines, as compared to T(H)2 cells, and was dependent on the strength and duration of the T cell receptor signal. Upon repeated restimulation (days 21-28) and differentiation, a switch occurred in which T(H)2 cells had high CCR7 expression, whereas T(H)1 cells lost CCR7 expression. Chronic (8 weeks and later) effector memory cell lines were 95% CCR7 negative. These data demonstrate an ordered pattern of CCR7 expression that suggest more rapid priming of T(H)1 cells in the lymph node, and delayed priming with prolonged CCR7 expression during T(H)2 responses, and may have implications for tracking T(H)1 effector T cells ex vivo in autoimmune diseases.
authors.affiliation: Department of Neurology, BRB 12-027, University of Maryland School of Medicine, 655 W. Baltimore St., Baltimore, MD 21201, USA. pcala001@umaryland.edu
authors.forename: Peter A
authors.initials: PA
authors.lastname: Calabresi
authors.forename: Rameeza
authors.initials: R
authors.lastname: Allie
authors.forename: Katherine M
authors.initials: KM
authors.lastname: Mullen
authors.forename: Sung Hae
authors.initials: SH
authors.lastname: Yun
authors.forename: Robert W
authors.initials: RW
authors.lastname: Georgantas
authors.forename: Katharine A
authors.initials: KA
authors.lastname: Whartenby
mesh_terms: D017951:Antigen Presentation / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D001327:Autoimmune Diseases / Q000276:immunology / Q000378:metabolism / Q000503:physiopathology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D016764:Cell Polarity / Q000276:immunology; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology*; D007700:Kinetics; D008213:Lymphocyte Activation / Q000276:immunology*; D054400:Receptors, CCR7; D019707:Receptors, Chemokine / Q000276:immunology* / Q000378:metabolism; D018417:Th1 Cells / Q000276:immunology* / Q000378:metabolism; D018418:Th2 Cells / Q000276:immunology* / Q000378:metabolism
chemical_list: D000954:Antigens, Surface; C515370:CCR7 protein, human; D016207:Cytokines; D054400:Receptors, CCR7; D019707:Receptors, Chemokine