title: Monitoring of DSP toxins in small-sized plankton fraction of seawater collected in Mutsu Bay, Japan, by ELISA method: relation with toxin contamination of scallop.
abstract: Monitorings were conducted on DSP toxins in mid-gut gland of scallop (mouse assay), cell numbers of toxic dinoflagellate species of Dinophysis, and diarrhetic shellfish poisoning (DSP) toxins in small-sized (0.7-5 microm) plankton fraction of seawater collected from surface (0 m) and 20 m depth at a station in Mutsu Bay, Aomori Prefecture, Japan, in 2000. A specific enzyme-linked immunosorbent assay (ELISA) was employed for the analysis of DSP toxins in small-sized plankton fraction using a mouse monoclonal anti-okadaic acid antibody which recognizes okadaic acid, dinophysistoxin-1, and dinophysistoxin-3. DSP toxins were detected twice in the mid-gut gland of scallops at 1.1-2.3 MU (mouse units) g(-1) on 26 June and at 0.6-1.2 MU g(-1) on 3 July, respectively. Relatively high cell densities of D. fortii were observed on 26 June and 11 September, and may only contribute to the bivalve toxicity during late June to early July. D. acuminata did not appear to be responsible for the toxicity of scallops in Mutsu Bay in 2000. ELISA monitoring of small-sized plankton fraction in seawater could detect DSP toxins two weeks before the detection of the toxin in scallops, and could do so two weeks after the loss of the bivalve toxicity by mouse assay. On 17 July, toxic D. fortii was detected at only small number, <10 cells l(-1), but DSP toxins were detected by the ELISA assay, suggesting a presence of other toxic small-sized plankton in seawater. For the purpose of reducing negative impacts of DSP occurrences, monitorings have been carried out hitherto on DSP toxins of bivalve tissues by mouse assay and on cell densities of "toxic" species of Dinophysis. Here we propose a usefulness of ELISA monitoring of plankton toxicity, especially in small-sized fraction, which are possible foods of mixotrophic Dinophysis, as a practical tool for detecting and predicting DSPs in coastal areas of fisheries grounds of bivalve aquaculture.
authors.affiliation: Laboratory of Marine Environmental Microbiology, Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan. imai1ro@kais.kyoto-u.ac.jp
authors.forename: Ichiro
authors.initials: I
authors.lastname: Imai
authors.forename: Hikaru
authors.initials: H
authors.lastname: Sugioka
authors.forename: Goh
authors.initials: G
authors.lastname: Nishitani
authors.forename: Tadashi
authors.initials: T
authors.lastname: Mitsuya
authors.forename: Yonekazu
authors.initials: Y
authors.lastname: Hamano
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D003967:Diarrhea / Q000209:etiology; D004141:Dinoflagellida / Q000472:pathogenicity*; D004784:Environmental Monitoring / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D005398:Fisheries; D005506:Food Contamination; D007564:Japan; D008387:Marine Toxins / Q000032:analysis*; D051379:Mice; D008974:Mollusca; D010933:Plankton; D012623:Seawater / Q000737:chemistry; D012680:Sensitivity and Specificity; D012758:Shellfish
chemical_list: D000911:Antibodies, Monoclonal; D008387:Marine Toxins