Dietary fructooligosaccharides induce immunoregulation of intestinal IgA secretion by murine Peyer's patch cells.
Probiotic supplements induce immunological responses in the host, and dietary fructooligosaccharides (FOS) stimulate the growth of selected intestinal microflora. In this study we investigated the immunological influences of orally administrated FOS. BALB/c mice were orally administered 0-7.5% FOS for 6 weeks, and the intestinal mucosal immune responses were measured. In the 2.5%-FOS group, fecal IgA was significantly increased. IgA secretion by Peyer's patch (PP) cells was upregulated in a dose-dependent way in response to FOS and CD4+ T cells from PP showed a dose-dependent increase in production of interferon-gamma and interleukin (IL) 10, and a high response in production of IL-5 and IL-6. In contrast, FOS suppressed serum IgG1. Our findings suggest that FOS supplementation changes the intestinal environment of microflora, and leads to upregulation of IgA secretion in CD4+ PP cells in intestinal mucosa, and to suppression of the systemic immune response to type 2 helper T (Th2) dominant.
Hosono
Akira
A
Department of Food Science and Technology, College of Bioresource Sciences, Nihon University, Fujisawa-shi, Kanagawa 252-8510, Japan. hosono@brs.nihon-u.ac.jp
Ozawa
Akane
A
Kato
Rina
R
Ohnishi
Yoshie
Y
Nakanishi
Yusuke
Y
Kimura
Teiji
T
Nakamura
Ryo
R
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D016207:Cytokines / Q000096:biosynthesis; D019587:Dietary Supplements; D005260:Female; D007070:Immunoglobulin A / Q000096:biosynthesis / Q000187:drug effects*; D007413:Intestinal Mucosa / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009844:Oligosaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D010581:Peyer's Patches / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D018418:Th2 Cells / Q000166:cytology; D015854:Up-Regulation
D000276:Adjuvants, Immunologic; D016207:Cytokines; D007070:Immunoglobulin A; D009844:Oligosaccharides; C116580:fructooligosaccharide