Early gene expression in the rat cortex after experimental traumatic brain injury and hypotension.
To determine c-fos and egr-1 mRNA levels in the rat cortex after traumatic brain injury (TBI) and TBI plus hemorrhagic hypotension (HH). Sprague-Dawley rats were subjected to TBI. HH was induced by removing 5 ml of blood from the jugular vein for a duration of 20 min. Cortical tissue was harvested at 0 and 120 min, and mRNA levels of the immediate early genes c-fos, egr-1 were obtained. Rats that sustained TBI alone had increased c-fos and egr-1 mRNA expression at 120 min that was significant. Additionally, c-fos mRNA levels after TBI alone were significantly higher than c-fos mRNA levels after TBI+HH at 120 min. Levels of egr-1 mRNA after TBI alone at 120 min were not significantly higher than egr-1 mRNA levels after TBI+HH at 120 min.
Awasthi
Deepak
D
Department of Neurosurgery, Louisiana State University Health Sciences Center, 1542 Tulane Avenue, New Orleans 70112, USA.
Kutz
Scott C
SC
Beuerman
Roger
R
Nguyen
Doan
D
Carey
Michael E
ME
Zeiller
Steve
S
D000818:Animals; D001930:Brain Injuries / Q000150:complications / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D051766:Early Growth Response Protein 1; D006470:Hemorrhage / Q000150:complications; D007022:Hypotension / Q000209:etiology / Q000378:metabolism*; D017874:Immediate-Early Proteins / Q000096:biosynthesis*; D008297:Male; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D014157:Transcription Factors / Q000096:biosynthesis*
D004268:DNA-Binding Proteins; D051766:Early Growth Response Protein 1; C486110:Egr1 protein, rat; D017874:Immediate-Early Proteins; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D014157:Transcription Factors