Apoptotic cell death and function of cryopreserved porcine hepatocytes in a bioartificial liver.
We have previously shown that cryopreservation leads to increased apoptotic death of porcine hepatocytes intended for use in a bioartificial liver (BAL). This study was designed to determine if a broad-spectrum caspase inhibitor, IDN-1965, reduced apoptosis and increased function of cryopreserved porcine hepatocytes in static culture or in a BAL. Porcine hepatocytes were studied immediately after isolation and after 2 weeks of cryopreservation in liquid nitrogen using medium supplemented with 25 micromol/L IDN-1965 or vehicle. Both apoptotic and necrotic cells were observed in cultures of fresh and cryopreserved hepatocytes, but the percentage of apoptotic cells increased after cryopreservation. Cryopreservation in IDN-1965 improved hepatocyte viability and reduced apoptotic cell death determined by TUNEL assay. Cryopreservation of hepatocytes in IDN-1965 was also associated with reduced caspase 3-like activity, decreased release of cytochrome c from mitochondria, and a slower decline in mitochondrial membrane potential after thawing. These markers of apoptosis were lowest after cryopreservation when IDN-1965 was added to both the culture and cryopreservation medium. Functional markers of hepatocyte activity (albumin production, diazepam metabolism, urea production) were also increased after cryopreservation and culture of hepatocytes in medium supplemented with 25 micromol/L IDN-1965. Cryopreservation of porcine hepatocytes in the presence of caspase inhibitor IDN-1965 was associated with reduced apoptosis and improved function of porcine hepatocytes in both static culture and a perfused BAL. These data demonstrate that inhibition of apoptosis also preserves cell function.
Matsushita
Takakazu
T
Division of Transplantation Surgery, Mayo Clinic, Rochester, MN 55905, USA.
Yagi
Toshikazu
T
Hardin
Joseph A
JA
Cragun
Jennifer D
JD
Crow
Frank W
FW
Bergen
H Robert
HR
Gores
Gregory J
GJ
Nyberg
Scott L
SL
D000418:Albumins / Q000378:metabolism; D000818:Animals; D014151:Anti-Anxiety Agents / Q000378:metabolism; D017209:Apoptosis / Q000502:physiology*; D053148:Caspase 3; D061945:Caspase Inhibitors; D020169:Caspases / Q000378:metabolism; D002478:Cells, Cultured; D015925:Cryopreservation; D045304:Cytochromes c / Q000378:metabolism; D003975:Diazepam / Q000378:metabolism; D022781:Hepatocytes / Q000378:metabolism* / Q000648:ultrastructure; D020287:In Situ Nick-End Labeling; D007211:Indoles / Q000378:metabolism; D019164:Liver, Artificial; D008928:Mitochondria / Q000378:metabolism; D009842:Oligopeptides / Q000378:metabolism; D013552:Swine; D014021:Tissue Preservation / Q000379:methods*; D014508:Urea / Q000378:metabolism
D000418:Albumins; D014151:Anti-Anxiety Agents; D061945:Caspase Inhibitors; D007211:Indoles; C118798:N-((1,3-dimethylindole-2-carbonyl)-valinyl)-3-amino-4-oxo-5-fluoropentanoic acid; D009842:Oligopeptides; D014508:Urea; D045304:Cytochromes c; D053148:Caspase 3; D020169:Caspases; D003975:Diazepam