Molecular cloning of ELOVL4 gene from cynomolgus monkey (Macaca fascicularis).
ELOVL4, elongation factor of very long chain fatty acids-4, is known to be responsible for autosomal dominant macular degeneration and Stargardt-like macular degeneration. In this study, we cloned the monkey homologue of ELOVL4 and determined the cellular and tissue distribution of the gene product. Sequence analysis of the monkey ELOVL4 gene revealed a high degree of homology between human and monkey. The cloned full-length cDNA of monkey ELOVL4 encoded 314 amino acids, the same length as human and two amino acids longer than mouse. The monkey ELOVL4 conserved the characteristics typical of the super family of ELO enzymes involved in the metabolism of membrane-bound fatty acid elongation. Real-time quantitative PCR demonstrated that the monkey ELOVL4 gene was highly expressed in restricted tissue-specific fashion, not only in the retina but also in the skin (90% of retina) and thymus (111% of retina). Immunohistochemical analysis detected signals predominantly in the photoreceptor layer of the monkey retina.
Umeda
Shinsuke
S
National Institute of Sensory Organs, National Tokyo Medical Center, Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo, Japan.
Ayyagari
Radha
R
Suzuki
Michihiro T
MT
Ono
Fumiko
F
Iwata
Fumino
F
Fujiki
Keiko
K
Kanai
Atsushi
A
Takada
Yuichiro
Y
Yoshikawa
Yasuhiro
Y
Tanaka
Yasuhiko
Y
Iwata
Takeshi
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005136:Eye Proteins / Q000235:genetics*; D007150:Immunohistochemistry; D008252:Macaca fascicularis; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D018076:DNA, Complementary; D005136:Eye Proteins; D008565:Membrane Proteins