Distribution of the small molecular weight GTP-binding proteins Rac1, Cdc42, RhoA and RhoB in the developing chick retina.
Immunohistochemistry was used to determine the distribution of Rac1, Cdc42, RhoA and RhoB GTPases during development of the chick retina. All proteins appear as early as embryonic day 5 (E5) in cells of the vitreal margin, E7-8 in cells of the inner third of the inner nuclear layer and E9-10 in photoreceptors. From E10 until hatching, RhoA, Rac1 and Cdc42 were seen in perikarya and/or processes of amacrine, ganglion cells, and photoreceptors. Rho proteins were also observed in retinal Müller cells, with different distributions. RhoB showed a transient expression, being severely down regulated after E18. The distribution pattern of Rho proteins during the development of the chick retina suggests a concerted role in the differentiation of specific cell types, and probably during synaptogenesis.
Santos-Bredariol
Andréa Silveira
AS
Department of Histology & Embryology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP 05508-900, Brazil.
Santos
Marinilce Fagundes
MF
Hamassaki-Britto
Dânia Emi
DE
D000818:Animals; D002642:Chick Embryo; D019204:GTP-Binding Proteins / Q000096:biosynthesis / Q000378:metabolism; D012160:Retina / Q000737:chemistry / Q000196:embryology / Q000378:metabolism*; D020764:cdc42 GTP-Binding Protein / Q000096:biosynthesis / Q000378:metabolism*; D020830:rac1 GTP-Binding Protein / Q000096:biosynthesis / Q000378:metabolism*; D020742:rhoA GTP-Binding Protein / Q000096:biosynthesis / Q000378:metabolism*; D020743:rhoB GTP-Binding Protein / Q000096:biosynthesis / Q000378:metabolism*
D019204:GTP-Binding Proteins; D020764:cdc42 GTP-Binding Protein; D020830:rac1 GTP-Binding Protein; D020742:rhoA GTP-Binding Protein; D020743:rhoB GTP-Binding Protein