Effect of Semax peptide on survival of cultured rat pheochromocytoma cells during oxidative stress.
We studied the effects of Semax (antiinsulin peptide with neuroprotective effect) on the survival of cultured rat pheochromocytoma cell after oxidative stress induced by short-term incubation with hydrogen peroxide. Studies with fluorescent dyes propidium iodide and Hoechst 33258 showed that cell incubation with hydrogen peroxide led to the formation of damaged cells with characteristic signs of necrosis. Semax dose-dependently reduced the number of cells damaged by oxidative stress. The efficiency of Semax depended on the time of its addition to the culture medium. The results suggest that the neuroprotective effect of Semax in ischemic stroke can be due to its capacity to protect neurons from damage caused by oxidative stress.
Safarova
E R
ER
Institute of Molecular Genetics, Russian Academy of Sciences, Moscow.
Shram
S I
SI
Zolotarev
Yu A
YA
Myasoedov
N F
NF
D000324:Adrenocorticotropic Hormone / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000818:Animals; D002470:Cell Survival / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005456:Fluorescent Dyes / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D009336:Necrosis; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress; D016716:PC12 Cells / Q000166:cytology / Q000187:drug effects; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats
D005456:Fluorescent Dyes; D018696:Neuroprotective Agents; D016877:Oxidants; D010446:Peptide Fragments; C048487:ACTH (4-7), Pro-Gly-Pro-; D000324:Adrenocorticotropic Hormone; D006861:Hydrogen Peroxide