Coeruleotrigeminal suppression of nociceptive sensorimotor function during inflammation in the craniofacial region of the rat.
Descending action from the locus coeruleus (LC) on the trigeminal sensorimotor function was evaluated in a rat model of oral-facial inflammation. For the induction of oral-facial inflammation, mustard oil (20% solution in 20microl mineral oil) was injected into the region of the temporomandibular joint (TMJ). One week before testing, rats received bilateral lesions of the LC using a cathodal current. The electromyogram (EMG) threshold, which is the threshold intensity for the onset of EMG activity of the masseter muscle evoked by pressure on the TMJ region, was used in the present study as an indicator of the trigeminal sensorimotor function. Following mustard oil injection, in the LC-lesioned rats, EMG thresholds significantly decreased at 30min, which lasted up to 240min. In contrast, EMG thresholds in the LC-intact rats returned to the level before injection after 180min. Systemic naloxone (1.3mg/kg, i.v.) produced a further decrease of EMG thresholds in both the LC-intact and LC-lesioned rats. Under the existence of naloxone, EMG thresholds in the LC-lesioned rats were significantly lower than those of the LC-intact rats. These results suggest that oral-facial inflammation activates the coeruleotrigeminal modulating system and that an action of this system is independent of the opioid depressive mechanism.
Matsutani
Kiyo
K
Department of Fixed Prosthodontics, Showa University School of Dentistry, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
Tsuruoka
Masayoshi
M
Shinya
Akiyuki
A
Furuya
Ryoichi
R
Kawawa
Tadaharu
T
Inoue
Tomio
T
D000818:Animals; D004195:Disease Models, Animal; D004576:Electromyography; D007249:Inflammation / Q000139:chemically induced / Q000503:physiopathology; D007270:Injections, Intra-Articular; D008125:Locus Coeruleus / Q000187:drug effects / Q000293:injuries / Q000502:physiology*; D008297:Male; D009149:Mustard Plant; D009270:Naloxone / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D017288:Pain Threshold / Q000187:drug effects; D010812:Physical Stimulation; D010936:Plant Extracts / Q000009:adverse effects; D010938:Plant Oils; D051381:Rats; D017207:Rats, Sprague-Dawley; D013704:Temporomandibular Joint / Q000187:drug effects / Q000294:innervation / Q000502:physiology*; D013997:Time Factors; D014278:Trigeminal Nuclei / Q000166:cytology / Q000502:physiology*
D009292:Narcotic Antagonists; D010936:Plant Extracts; D010938:Plant Oils; D009270:Naloxone; C027793:mustard oil