[Tissue microarray and in situ hybridization study on hypoxia inducible factor 1alpha mRNA expression in epithelial ovarian tumor].
OBJECTIVE
To study the expression and significance of hypoxia inducible factor 1alpha (HIF-1alpha) in epithelial ovarian tumors.


METHODS
The expression of HIF-1alpha mRNA in 295 patients with epithelial ovarian tumor was analyzed retrospectively by high-throughput tissue microarray and in situ hybridization, which was compared with 13 normal ovarian tissue samples.


RESULTS
The expression rates of HIF-1alpha mRNA were 0, 13.2%, 42.1% and 81.9% in normal ovarian tissue, benign, borderline and malignant ovarian tumors. Expression rate of HIF-1alpha mRNA in borderline and invasive tumor was significantly higher than those in normal ovarian tissue and benign tumor (P < 0.001). Statistical analysis revealed that the expression of HIF-1alpha mRNA was not related to FIGO stages or histological subtypes. Close negative relation was observed between the expression of HIF-1alpha mRNA and tumor histological differentiation (P < 0.001).


CONCLUSIONS
The overexpression of HIF-1alpha may play an important role in oncogenesis of epithelial ovarian tumor. Tissue microarray is an efficient technique of molecular biology.
Luo
Jun
J
Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Peng
Zhi-lan
ZL
Wang
He
H
Cheng
Yi-fan
YF
Yang
Kai-xuan
KX
Xie
Lan
L
Qian
Xiao-lei
XL
Zou
Yan
Y
D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000032:analysis / Q000235:genetics*; D017403:In Situ Hybridization / Q000379:methods*; D008875:Middle Aged; D009375:Neoplasms, Glandular and Epithelial / Q000737:chemistry / Q000378:metabolism*; D010051:Ovarian Neoplasms / Q000737:chemistry / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis*; D046888:Tissue Array Analysis / Q000379:methods*
C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D012333:RNA, Messenger