Feasibility of atmospheric pressure desorption/ionization on silicon mass spectrometry in analysis of drugs.
The feasibility of atmospheric pressure desorption/ionization on silicon mass spectrometry (AP-DIOS-MS) for drug analysis was investigated. It was observed that only compounds with relative high proton affinity are efficiently ionized under AP-DIOS conditions. The limits of detection (LODs) achieved in MS mode with midazolam, propranolol, and angiotensin II were 80 fmol, 20 pmol, and 1 pmol, respectively. In MS/MS mode the LODs for midazolam and propranolol were 10 fmol and 5 pmol, respectively. The good linearity (r(2) > 0.991), linear dynamic range of 3 orders of magnitude, and reasonable repeatability showed that the method is suitable for quantitative analysis.
Huikko
K
K
Viikki Drug Discovery Technology Center, Department of Pharmacy, P.O. Box 56, FIN-00014 University of Helsinki, Helsinki, Finland.
Östman
P
P
Viikki Drug Discovery Technology Center, Department of Pharmacy, P.O. Box 56, FIN-00014 University of Helsinki, Helsinki, Finland.
Sauber
C
C
Agilent Technologies, Deutschland GmbH, Hewlett-Packard-Straße 8, 76337 Waldbronn, Germany.
Mandel
F
F
Agilent Technologies, Deutschland GmbH, Hewlett-Packard-Straße 8, 76337 Waldbronn, Germany.
Grigoras
K
K
Microelectronics Centre, Helsinki University of Technology, P.O. Box 3000, FIN-02015 HUT, Finland.
Franssila
S
S
Microelectronics Centre, Helsinki University of Technology, P.O. Box 3000, FIN-02015 HUT, Finland.
Kotiaho
T
T
Viikki Drug Discovery Technology Center, Department of Pharmacy, P.O. Box 56, FIN-00014 University of Helsinki, Helsinki, Finland.
Kostiainen
R
R
Viikki Drug Discovery Technology Center, Department of Pharmacy, P.O. Box 56, FIN-00014 University of Helsinki, Helsinki, Finland.|Division of Pharmaceutical Chemistry, Department of Pharmacy, P.O. Box 56, FIN-00014 University of Helsinki, Helsinki, Finland.
D000082:Acetaminophen / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D000390:Air Ionization; D001274:Atmospheric Pressure; D002264:Carboxylic Acids / Q000032:analysis / Q000737:chemistry; D007660:Ketoprofen / Q000032:analysis / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D008874:Midazolam / Q000032:analysis / Q000737:chemistry; D015394:Molecular Structure; D009280:Naphthaleneacetic Acids / Q000032:analysis / Q000737:chemistry; D009281:Naphthalenes / Q000032:analysis / Q000737:chemistry; D009285:Naphthoquinones / Q000032:analysis / Q000737:chemistry; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000737:chemistry; D011433:Propranolol / Q000032:analysis / Q000737:chemistry; D012825:Silicon / Q000737:chemistry*; D013739:Testosterone / Q000032:analysis / Q000737:chemistry
D002264:Carboxylic Acids; D009280:Naphthaleneacetic Acids; D009281:Naphthalenes; D009285:Naphthoquinones; D004364:Pharmaceutical Preparations; C085312:1-naphthoic acid; D000082:Acetaminophen; D013739:Testosterone; C020424:2-naphthylacetic acid; C037386:acetaminophen glucuronide; D007660:Ketoprofen; D011433:Propranolol; D008874:Midazolam; C035342:1,4-naphthoquinone; D012825:Silicon