Rapid quantitation of Trypanosoma cruzi in host tissue by real-time PCR.
A real-time PCR technique that allows for accurate and sensitive quantitation of tissue parasite burden in animals infected with the protozoan parasite Trypanosoma cruzi was developed. The utility of this method was demonstrated by confirmation of higher parasite load in mice with acute infections in comparison to chronically infected mice, detection of tissue-restricted parasite persistence in different parasite:host strain combinations, and the observation of increased tissue parasite burden with higher infective doses. This method should be a useful tool for monitoring parasite burden in hosts under various treatment regimens.
Cummings
Kara L
KL
Department of Cellular Biology, Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA.
Tarleton
Rick L
RL
D000818:Animals; D017931:DNA Primers; D005260:Female; D006321:Heart / Q000469:parasitology; D006790:Host-Parasite Interactions; D051379:Mice; D008810:Mice, Inbred C57BL; D018482:Muscle, Skeletal / Q000469:parasitology; D016133:Polymerase Chain Reaction / Q000379:methods*; D013997:Time Factors; D014349:Trypanosoma cruzi / Q000235:genetics / Q000302:isolation & purification*
D017931:DNA Primers