[Preliminary study on effect of ganhuang injection on reject reaction of xenograft].
OBJECTIVE
To study the effect of Ganhuang Injection (GHI) on reject reaction of xenograft.


METHODS
RT-PCR technique was used to detect the activated relevant gene expression in porcine endothelial cells (PEC), and 51Cr releasing method was used to test the killing and adhesion action of NK cells.


RESULTS
The normal human serum and human NK-92 cell could up-regulate the mRNA expressions of E-selectin and IL-1 alpha gene in PEC, showing the PEC activating action, GHI could inhibit these activated gene expressions. Cyto-toxic experiment showed that GHI could also inhibit the cytotoxicity of NK cell on PEC dose-dependently, which was in accord with its inhibition on adhesive action of NK on PEC.


CONCLUSIONS
GHI could inhibit not only the PEC activation in hyperacute rejection, but also the function of NK cells in delayed xenograft rejection.
Shi
Bin
B
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100080.
Yin
Hui-jun
HJ
Huang
Xiu-ying
XY
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D019040:E-Selectin / Q000096:biosynthesis / Q000235:genetics; D004730:Endothelium, Vascular / Q000166:cytology; D006035:Glycyrrhiza / Q000737:chemistry*; D006084:Graft Rejection; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis / Q000235:genetics; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012250:Rheum / Q000737:chemistry*; D013552:Swine
D004365:Drugs, Chinese Herbal; D019040:E-Selectin; D007375:Interleukin-1; D012333:RNA, Messenger