Identification of AMSH-LP containing a Jab1/MPN domain metalloenzyme motif.
We have isolated a cDNA clone encoding a new AMSH (associated molecule with the SH3 domain of STAM) family protein, termed AMSH-like protein (AMSH-LP). AMSH-LP has similar characteristics to AMSH; both AMSH-LP and AMSH are expressed ubiquitously in various human tissues, contain a putative nuclear localization signal (NLS), an Mpr/Pad1/N-terminal (MPN) domain, and a Jab1/MPN domain metalloenzyme (JAMM) motif in their structures, and are excluded from the nucleus when lacking either the NLS or MPN domain. Moreover, we observed an enhancement of interleukin 2 (IL-2)-mediated c-myc induction in AMSH-LP-transfected cells similar to that seen in AMSH-transfected cells, suggesting a functional similarity between AMSH-LP and AMSH. However, the present study demonstrated that AMSH-LP, unlike AMSH, fails to bind to the SH3 domains of STAM1 (signal transducing adaptor molecule 1) and Grb2. These results suggest that AMSH-LP and AMSH may have different functions.
Kikuchi
Kazu
K
Department of Immunology and Microbiology, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, 980-8575, Sendai, Japan.
Ishii
Naoto
N
Asao
Hironobu
H
Sugamura
Kazuo
K
D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D003001:Cloning, Molecular; D010450:Endopeptidases; D056827:Endosomal Sorting Complexes Required for Transport; D005786:Gene Expression Regulation; D016259:Genes, myc / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D014018:Tissue Distribution; D043222:Ubiquitin Thiolesterase; D018909:src Homology Domains / Q000235:genetics
D002352:Carrier Proteins; D056827:Endosomal Sorting Complexes Required for Transport; D011993:Recombinant Fusion Proteins; C486696:STAMBP protein, human; D010450:Endopeptidases; D010447:Peptide Hydrolases; C476302:STAMBPL1 protein, human; D043222:Ubiquitin Thiolesterase