[Bio-panning of antagonistic peptides against HAb18G/CD147 and their function of anti-hepatoma invasion].
OBJECTIVE
To screen out the HAb18G/CD147 binding peptides and find out an antagonist against hepatoma invasion.


METHODS
HAb18G/CD147 was purified by affinity chromatographic method and the antigen binding peptides acquired by bio-panning a phage-displayed 12-peptide library. After obtaining the sequence of the selected phage-displayed peptides, all the 9 peptides were synthesized by solid-phase method and identified by mass spectrograph. The peptides' anti-metastatic function was tested by Boyden Chamber assay.


RESULTS
The purified HAb18G/CD147, identified by Western blot (molecular weight about 65 kd) could be used to bio-pan the phage-displayed peptide library. After 3 rounds of bio-panning, 9 positive phage clones were selected and sequenced. The synthesized peptides had uneven inhibitory activities and three of them were able to markedly inhibit the hepatoma cell invasion (P < 0.01). The most effective peptide decreased by 90.1% of hepatoma cells migrating through the Boyden Chamber membrane as compared with the control.


CONCLUSIONS
Bio-panning the phage-displayed peptide library can be used successfully to screen out the antigen binding peptides. Hepatoma metastatic potential can be inhibited by peptide antagonist which could be a good foundation of developing peptide therapeutic agent against hepatoma metastasis.
Huang
Bao-cheng
BC
Cell Engineering Research Center, Fourth Military Medical University, Xi'an 710032, China.
Shang
Peng
P
Qian
Ai-rong
AR
Wang
Xian-hui
XH
Shi
Guang-hua
GH
Chen
Zhi-nan
ZN
D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D051926:Basigin / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000473:pathology; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000473:pathology; D051379:Mice; D009361:Neoplasm Invasiveness; D019151:Peptide Library; D010455:Peptides / Q000627:therapeutic use*
D000911:Antibodies, Monoclonal; D019151:Peptide Library; D010455:Peptides; D051926:Basigin