Identification of Neisseria meningitidis serogroups Y and W135 by siaD nucleotide sequence analysis.
Rapid serogrouping of meningococci is essential for the effective public health management of cases of the disease and the contacts of infected patients. Here we describe an accurate nucleotide-sequencing method for the confirmation of serogroup Y and W135 meningococci by siaD gene analysis from cultures of Neisseria meningitidis.
Lewis
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C
Scottish Centre for Infection and Environmental Health, Clifton House, Clifton Place, Glasgow G3 7LN, Scotland.
Clarke
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SC
D015373:Bacterial Typing Techniques; D001483:Base Sequence; D003470:Culture Media; D006801:Humans; D008585:Meningitis, Meningococcal / Q000382:microbiology; D008589:Meningococcal Infections / Q000382:microbiology*; D008969:Molecular Sequence Data; D038582:Neisseria meningitidis, Serogroup W-135 / Q000145:classification* / Q000235:genetics; D038581:Neisseria meningitidis, Serogroup Y / Q000145:classification* / Q000235:genetics; D017421:Sequence Analysis; D017422:Sequence Analysis, DNA; D012703:Serotyping; D012799:Sialyltransferases / Q000235:genetics*
D003470:Culture Media; D012799:Sialyltransferases