Hemostatic effects of polymerized albumin particles bearing rGPIa/IIa in thrombocytopenic mice.
The recombinant fragment of the platelet membrane glycoprotein Ia/IIa (rGPIa/IIa) was conjugated to the polymerized albumin particles (polyAlb) with the average diameter of 180 nm. The intravenous administration of rGPIa/IIa-polyAlb to thrombocytopenic mice ([platelet] = 2.1+/-0.3 x 10(5) particles/ microL) with three doses of ca. 2.4 x 10(10), 7.2 x 10(10), and 2.4 x 10(11)particles/kg, respectively, significantly reduced their bleeding time to 426+/-71, 378+/-101, and 337+/-46 s, respectively, whereas that of the control groups (PBS) was 730+/-198 s. The injection of rGPIa/IIa-polyAlb (2.4 x 10(11)particles/kg) was approximately equal to the effect of the injection of the mouse platelets at a dose of 2.0 x 10(10) particles/kg. It was confirmed that rGPIa/IIa-polyAlb had a recognition ability against collagen and could contribute to the hemostasis in the thrombocytopenic mice as a platelet substitute.
Teramura
Yuji
Y
Advanced Research Institute for Science and Engineering, Waseda University, 169-8555, Tokyo, Japan.
Okamura
Yosuke
Y
Takeoka
Shinji
S
Tsuchiyama
Hiromi
H
Narumi
Hideki
H
Kainoh
Mie
M
Handa
Makoto
M
Ikeda
Yasuo
Y
Tsuchida
Eishun
E
D000818:Animals; D003094:Collagen / Q000378:metabolism; D005260:Female; D006489:Hemostatic Techniques; D006490:Hemostatics / Q000627:therapeutic use*; D006801:Humans; D066298:In Vitro Techniques; D038982:Integrin alpha2beta1 / Q000008:administration & dosage / Q000627:therapeutic use*; D051379:Mice; D008807:Mice, Inbred BALB C; D010316:Particle Size; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000627:therapeutic use; D012709:Serum Albumin / Q000008:administration & dosage / Q000627:therapeutic use; D013921:Thrombocytopenia / Q000628:therapy*
D006490:Hemostatics; D038982:Integrin alpha2beta1; D011994:Recombinant Proteins; D012709:Serum Albumin; D003094:Collagen