title: Effects of the CDT6/ANGX gene on tumour growth in immune competent mice.
abstract: BACKGROUND
  Cornea-derived transcript 6 (CDT6, also known as AngX) has been described to inhibit tumour growth in a human melanoma growing in nude mice. In another report, however, enhancement of tumour growth in comparable circumstances occurred. We therefore studied the effect of the same gene in different circumstances, using an immune competent mouse model.
  
  
  METHODS
  In this report we describe the generation of a stably CDT6-expressing clone of the murine melanoma cell line B16-F10. These cells were implanted in female C57/B16 mice with empty vector transfected cells as control.
  
  
  RESULTS
  We found no significant inhibition or stimulation of either lag-time or doubling-time of the tumours. In addition no effect of the CDT6 gene product was found on proliferation of endothelial cells in vitro.
  
  
  CONCLUSIONS
  In contrast to an immune deficient mouse model, no anti-tumour effect could be detected with the CDT6 gene product in an immune competent mouse model.
authors.affiliation: Department of Medical Oncology, University Hospital Groningen, P.O. Box 30001, 9700 RB Groningen, The Netherlands.
authors.forename: Diane
authors.initials: D
authors.lastname: Bouïs
authors.forename: Geke A P
authors.initials: GA
authors.lastname: Hospers
authors.forename: Coby
authors.initials: C
authors.lastname: Meijer
authors.forename: Wendy
authors.initials: W
authors.lastname: Dam
authors.forename: Ron
authors.initials: R
authors.lastname: Peek
authors.forename: Nanno H
authors.initials: NH
authors.lastname: Mulder
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000627:therapeutic use*; D042501:Angiogenic Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D004730:Endothelium, Vascular / Q000187:drug effects / Q000473:pathology; D005260:Female; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D007121:Immunocompetence; D008546:Melanoma, Experimental / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D012333:RNA, Messenger / Q000378:metabolism; D012334:RNA, Neoplasm / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
chemical_list: D043925:Angiogenesis Inducing Agents; D042501:Angiogenic Proteins; D000970:Antineoplastic Agents; D012333:RNA, Messenger; D012334:RNA, Neoplasm