Stability of repetitive-sequence PCR patterns with respect to culture age and subculture frequency.
To examine the stability of repetitive-sequence (rep) PCR profiles, six species of bacteria were subcultured to blood agar plates and DNA was extracted from the cultures after 24, 48, and 72 h of incubation at 35 degrees C. In addition, the same species were subcultured to fresh blood plates daily and DNA was extracted from the cultures after growth of 5, 10, and 15 subcultures, respectively. rep PCR analysis demonstrated that all rep PCR fingerprints from a single species were identical.
Kang
Hyunseok Peter
HP
Division of Laboratory Medicine, Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Dunne
W Michael
WM
D001424:Bacterial Infections / Q000382:microbiology; D015373:Bacterial Typing Techniques; D003470:Culture Media; D016172:DNA Fingerprinting / Q000379:methods; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D006090:Gram-Negative Bacteria / Q000235:genetics / Q000254:growth & development*; D006094:Gram-Positive Bacteria / Q000235:genetics / Q000254:growth & development*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D012692:Serial Passage
D003470:Culture Media; D004269:DNA, Bacterial