Development and validation of a liquid chromatographic method for Casiopeina IIgly in rat plasma.
A sensitive and specific liquid chromatographic method using solid-phase extraction with Sep-pak cartridges has been developed for the determination of Casiopeina IIgly and validated over the linear range 2.5-50 microg/ml in rat plasma. The analysis was performed on a Symetry C(18) (5 microm) column with a Phenomenex C(18) precolumn. The mobile phase was methanol-water (58:42, v/v). The column effluent was monitored at 273 nm. The results showed that the assay is sensitive at 2.5 microg/ml. Maximum intra-day coefficient of variation was 11.47%. The recovery based upon addition of internal standard to rat plasma was 80.98%. The method was used to perform preclinical pharmacokinetic studies in rat plasma and was found to be satisfactory.
Reyes
Leobardo
L
Departamento de Farmacia, Universidad Nacional Autónoma de México, México DF 04510, Mexico.
Fuentes-Noriega
Inés
I
Ruiz-Ramìrez
Lena
L
Macías
Lucía
L
D000818:Animals; D002138:Calibration; D003300:Copper / Q000097:blood*; D008297:Male; D009942:Organometallic Compounds / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D012015:Reference Standards; D012680:Sensitivity and Specificity
D009942:Organometallic Compounds; C409443:casiopeina II; D003300:Copper