Production and expression of Gro-alpha and RANTES by peripheral blood mononuclear cells isolated from patients with Kawasaki disease and measles.
We investigated whether the production and gene expression of Gro-alpha and RANTES in Kawasaki disease differ in measles. Forty-two samples from 14 patients in different clinical stages of Kawasaki disease, eight samples from 8 patients in the acute stage of measles and seven samples from 7 healthy children were collected. The present study was performed using ELISA and RT-PCR for the productions and gene expression of the chemokines. The production of Gro-alpha was markedly elevated during the acute stage of measles compared with Kawasaki disease. Moreover, the expression of Gro-alpha was increased in every case of measles, but not in Kawasaki disease. The production of RANTES was elevated in the acute stage of both diseases when compared to the healthy control. However, the plasma RANTES level did not change significantly according to the clinical stages of Kawasaki disease. A correlation between the production and gene expression of RANTES and Gro-alpha was not found in Kawasaki disease. These results suggest that Kawasaki disease differs from measles with regard to Gro-alpha production and expression, but not RANTES. Gro-alpha might play an important role in the acute stage of measles, however not in Kawasaki disease. Further studies are needed to clarify the efficacy of Gro-alpha as a marker in measles.
Kim
Hee Sun
HS
Department of Pediatrics & Microbiology, Yeungnam University School of Medicine, Daegu, Korea.
Kim
Won Duk
WD
Lee
Young Hwan
YH
D015415:Biomarkers; D018946:Chemokine CCL5 / Q000097:blood / Q000235:genetics*; D054360:Chemokine CXCL1; D018925:Chemokines / Q000097:blood / Q000235:genetics*; D002630:Chemotactic Factors / Q000097:blood / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D005260:Female; D015870:Gene Expression / Q000276:immunology; D006801:Humans; D007223:Infant; D036341:Intercellular Signaling Peptides and Proteins / Q000097:blood / Q000235:genetics*; D007963:Leukocytes, Mononuclear / Q000502:physiology*; D008297:Male; D008457:Measles / Q000276:immunology* / Q000503:physiopathology; D009080:Mucocutaneous Lymph Node Syndrome / Q000276:immunology* / Q000503:physiopathology; D012333:RNA, Messenger / Q000032:analysis
D015415:Biomarkers; C475896:CXCL1 protein, Bos taurus; D018946:Chemokine CCL5; D054360:Chemokine CXCL1; D018925:Chemokines; D002630:Chemotactic Factors; D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger