Codon 201 polymorphism of DCC gene is a prognostic factor in patients with colorectal cancer.
The polymorphism at codon 201 of the "deleted in colorectal carcinoma" (DCC) gene has been liked to susceptibility to colorectal cancer. However, its clinicopathological significance has not been reported. We examined the codon 201 polymorphism and loss of heterozygosity (LOH) by PCR-restriction fragment length polymorphism (PCR-RFLP) in 59 colorectal cancers, 48 samples from transitional mucosa and 67 samples from normal mucosa. The frequencies of the polymorphism did not significantly differ from normal to transitional mucosa and to tumor, but LOH was increased from transitional mucosa to tumor. Almost all of the LOH cases showed the polymorphism. The polymorphism was increased from well/moderately to poorly differentiated and to mucinous carcinoma (P=0.03). The polymorphism was more frequently seen in advanced stages than in earlier stages (P=0.02), and further predicted worse survival (P=0.04). The data suggest that the codon 201 polymorphism of the DCC gene was a target of LOH, and predicted prognosis in colorectal cancer patients.
Zhang
Hong
H
Department of Dermatology, Institute of Biomedicine and Surgery, University of Linköping, S-581 85 Linköping, Sweden.
Arbman
Gunnar
G
Sun
Xiao-Feng
XF
D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D003062:Codon; D015179:Colorectal Neoplasms / Q000235:genetics*; D005260:Female; D016163:Genes, DCC / Q000235:genetics*; D006801:Humans; D019656:Loss of Heterozygosity; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D011379:Prognosis
D003062:Codon