Specific quantification of human genomes from low copy number DNA samples in forensic and ancient DNA studies.
We reviewed the current methodologies used for human DNA quantitation in forensic and ancient DNA studies, including sensitive hybridization methods based on the detection of nuclear alpha-satellite repetitive DNA regions or more recently developed fluorogenic real-time polymerase chain reaction (PCR) designs for the detection of both nuclear and mitochondrial DNA regions. Special emphasis has been put on the applicability of recently described different real-time PCR designs targeting different fragments of the HV1 mtDNA control region, and a segment of the X-Y homologous amelogenin gene. The importance of these quantitative assays is to ensure the consistency of low copy number DNA typing (STR profiling and mtDNA sequencing).
Alonso
Antonio
A
Instituto Nacional de Toxicología, Servicio de Biología, Luis Cabrera 9, 28002 Madrid, Spain. a.alonso@mju.es
Martin
Pablo
P
Albarrán
Cristina
C
García
Pilar
P
Primorac
Dragan
D
García
Oscar
O
Fernández de Simón
Lourdes
L
García-Hirschfeld
Julia
J
Sancho
Manuel
M
Fernández-Piqueras
Jose
J
D053523:Amelogenin; D022801:Complementarity Determining Regions; D016172:DNA Fingerprinting / Q000379:methods*; D004272:DNA, Mitochondrial / Q000235:genetics; D003746:Dental Enamel Proteins / Q000235:genetics; D018732:Forensic Anthropology / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D012732:Sex Determination Analysis / Q000379:methods; D020080:Tandem Repeat Sequences
D053523:Amelogenin; D022801:Complementarity Determining Regions; D004272:DNA, Mitochondrial; D003746:Dental Enamel Proteins