[3H]Dopamine release in striatum in response to cortical stimulation in a corticostriatal slice preparation.
A new method has been developed to investigate corticostriatal glutamatergic influence on [3H]dopamine release in striatum in complex corticostriatal slice preparation in vitro. Horizontal slices containing the striatum and the adjacent prefrontal cortex of rat brain were cut in a plane that maintains corticostriatal connections. After incubation with [3H]dopamine, slices were submerged in a two-compartment bath so that the cortical region was contained entirely in one compartment, corpus callosum passed through a silicone greased slot, and the striatal region was contained in the other compartment. A cannula was placed just above the striatal part of the slice and effluent was collected with a peristaltic pump, released tritiated materials were counted with a liquid scintillation counter. Electric field stimulation of cortex increased the release of [3H]dopamine in the striatum. Bicuculline (1 mM) increased the basal and stimulated release of [3H]dopamine in the striatum in response to cortical stimulation of cortex indicating the GABAergic control on dopamine release. This method allows investigation of the effect of cortical stimulation on glutamate-dopamine-GABA interactions in the striatum in vitro that might help to understand better the neurochemical background of schizophrenia or Parkinson's disease.
Juranyi
Zsolt
Z
Divison of Preclinical Research, Pharmacological Laboratory I., EGIS Pharmaceuticals Ltd., Bökényföldi út 116, H-1475, PO Box 100, 10, Budapest, Hungary.
Zigmond
Michael J
MJ
Harsing
Laszlo G
LG
D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D046508:Culture Techniques / Q000295:instrumentation* / Q000379:methods; D004298:Dopamine / Q000493:pharmacokinetics*; D004558:Electric Stimulation; D004867:Equipment Design; D008297:Male; D009434:Neural Pathways / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D011189:Potassium Chloride / Q000494:pharmacology; D017397:Prefrontal Cortex / Q000187:drug effects / Q000378:metabolism / Q000502:physiology* / Q000528:radiation effects; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D051381:Rats; D013268:Stimulation, Chemical; D013779:Tetrodotoxin / Q000494:pharmacology
D019275:Radiopharmaceuticals; D013779:Tetrodotoxin; D011189:Potassium Chloride; D004298:Dopamine; D001640:Bicuculline