title: Establishment of a new human malignant fibrous histiocytoma cell line, FU-MFH-1: cytogenetic characterization by comparative genomic hybridization and fluorescence in situ hybridization.
abstract: Although a number of malignant fibrous histiocytoma (MFH) cell lines have been reported, their characterization at a molecular cytogenetic level has not been fully established. In this study, we established a new human cell line, designated as FU-MFH-1, from a storiform-pleomorphic MFH arising in the retroperitoneum of a 61-year-old woman, and applied comparative genomic hybridization (CGH) and fluorescence in situ hybridization (FISH) with chromosome painting probes for the characterization of chromosome alterations. FU-MFH-1 cells were spindle, round, or polygonal in shape with oval nuclei, and were maintained continuously in vitro for over 50 passages for more than 12 months. G-banding analysis was performed and FU-MFH-1 revealed a complex karyotype with an abnormal chromosome 19 containing a homogeneously staining region (hsr). CGH analysis showed a high-level amplification of 12q13-->q21. The high-level amplification detected by CGH was refined by FISH. These results showed that the hsr was composed of amplified DNA sequences from 12q. Our study emphasizes the usefulness of CGH as a powerful tool for chromosomal localization of amplified sequences. The FU-MFH-1 cell line should be useful for biologic and molecular pathogenetic investigations of human MFH.
authors.affiliation: Department of Pathology, School of Medicine, Fukuoka University, Fukuoka, Japan. nishio@minf.med.fukuoka-u.ac.jp
authors.forename: Jun
authors.initials: J
authors.lastname: Nishio
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Iwasaki
authors.forename: Masako
authors.initials: M
authors.lastname: Ishiguro
authors.forename: Yuko
authors.initials: Y
authors.lastname: Ohjimi
authors.forename: Noriko
authors.initials: N
authors.lastname: Nishimura
authors.forename: Takamasa
authors.initials: T
authors.lastname: Koga
authors.forename: Tatsuhiko
authors.initials: T
authors.lastname: Kawarabayashi
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Kaneko
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kikuchi
mesh_terms: D000818:Animals; D005260:Female; D018219:Histiocytoma, Benign Fibrous / Q000235:genetics / Q000473:pathology*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007621:Karyotyping; D051379:Mice; D016513:Mice, SCID; D008875:Middle Aged; D009693:Nucleic Acid Hybridization / Q000379:methods*; D014407:Tumor Cells, Cultured