Cell volume changes during rapid temperature shifts.
The effect of a rapid temperature increase on the volume of different types of cells was investigated. Experiments were carried out using continuous microscopic image analysis. Volume variation of yeast cells, yeast spheroplasts and human leukaemia cells was measured during the transient phase after a thermal shift. The thermal shift was found to induce rapid increase in cell volume for cells lacking a cell wall (yeast spheroplasts and human leukaemia cells). This increase in cell volume is assumed to be a main cause of the heat shock-induced cell death. A theoretical mechanistic model that explains the behaviour of these cells is finally proposed.
Gervais
Patrick
P
Laboratoire de Génie des Procédés Alimentaires et Biotechnologiques, ENSBANA, 21000 Dijon, France. gervais@u-bourgogne.fr
Martínez de Marañon
Iñigo
I
Evrard
Christine
C
Ferret
Eric
E
Moundanga
Sylvie
S
D048429:Cell Size / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D018869:Heat-Shock Response / Q000502:physiology* / Q000528:radiation effects; D006358:Hot Temperature; D006801:Humans; D020014:K562 Cells; D040542:Mechanotransduction, Cellular; D008954:Models, Biological; D015203:Reproducibility of Results; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000528:radiation effects; D013104:Spheroplasts / Q000166:cytology* / Q000528:radiation effects; D013696:Temperature