Serine protease activity in excretory-secretory products of Oestrus ovis (Diptera: Oestridae) larvae.
The sheep bot fly, Oestrus ovis, is a very common myiasis of nasal and sinus cavities of sheep and goats causing severe welfare and production implications. As the viability of O. ovis adult flies strictly depends on larval abilities to assimilate and to stock nutrients from the host, it was necessary to investigate proteolytic activities in larval excretory/secretory products (ESP). ESP of O. ovis larvae degrade mucosal and plasmatic components such as mucin, albumin or immunoglobulin G. A preliminary biochemical characterization, using substrate gel analysis and inhibitor sensitivity, demonstrated the presence of at least six major serine proteases (molecular weights from 20 to 100 kDa), mainly trypsin-like, secreted in the digestive tube of larvae. Their involvement in larval trophic activity and evasion from the host immune response is further discussed as O. ovis excretory/secretory serine proteases could represent potential vaccinal targets.
Tabouret
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G
UMR INRA/DGER 1225 Interactions Hôtes-Agents Pathogènes, Ecole Nationale Vétérinaire de Toulouse, 23 Chemin des Capelles, 31076 Toulouse Cedex 03, France.
Bret-Bennis
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L
Dorchies
Ph
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Jacquiet
Ph
P
D000818:Animals; D001391:Azo Compounds / Q000378:metabolism; D001586:Benzoylarginine Nitroanilide / Q000378:metabolism; D002417:Cattle; D002863:Chromogenic Compounds / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D004175:Diptera / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007074:Immunoglobulin G / Q000378:metabolism; D007814:Larva / Q000201:enzymology; D008970:Molecular Weight; D009077:Mucins / Q000378:metabolism; D009198:Myiasis / Q000469:parasitology / Q000662:veterinary; D011480:Protease Inhibitors / Q000494:pharmacology; D012697:Serine Endopeptidases / Q000032:analysis* / Q000737:chemistry / Q000502:physiology; D012709:Serum Albumin / Q000378:metabolism; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology
D001391:Azo Compounds; D002863:Chromogenic Compounds; D007074:Immunoglobulin G; D009077:Mucins; D011480:Protease Inhibitors; D012709:Serum Albumin; C028470:Azocoll; D003094:Collagen; D001586:Benzoylarginine Nitroanilide; D012697:Serine Endopeptidases