title: Dose confirmation of moxidectin 0.5% pour-on against adults and fourth-stage larvae of various Cooperia spp. and Trichostrongylus colubriformis in Louisiana.
abstract: Thirty-five castrate or intact Holstein male calves, ranging in weight from 122 to 210kg, were used in the study. On study Days -15 and -14, all study calves were treated with fenbendazole 10% paste at 10mg/kg to remove existing nematode infections. All cattle had zero egg counts on Day -1. Experimental infections of a mixed species inoculum were administered to all calves on Day 0. The inoculum consisted of Cooperia spp. (primarily C. punctata, but also C. pectinata, C. spatulata, C. oncophora and C. surnabada-total 40,961); Ostertagia ostertagi-1550; Trichostrongylus colubriformis-4996; and Oesophagostomum radiatum-38. Necropsy results from two of three monitor calves slaughtered on Day 6 after infection indicated that Day 6 was an appropriate time to evaluate efficacy of moxidectin against fourth larval stages (L(4)). The remaining 32 calves were randomly allotted to four groups of eight based on body weights. Eight calves (Group 1) were treated with moxidectin 0.5% pour-on at 0.5mg/kg on Day 6 to evaluate efficacy against nematode larval stages; eight control calves (Group 2) were matched with these principals. Eight calves (Group 3) were also treated with moxidectin pour-on at the same dosage on Day 23 in order to determine efficacy against adult nematodes; eight control calves (Group 4) were matched with these principals. In both cases, principals and controls of the Days 6 and 23 treatments were necropsied at 14-15 days after treatment. C. punctata was the only species found in a sufficient number of controls to evaluate moxidectin efficacy against the L(4). Moxidectin pour-on was not effective (P<0.05) against C. punctata L(4) by arithmetic means, but was highly effective based on geometric means. Regardless of whether arithmetic or geometric means were used for percent efficacy calculations, moxidectin pour-on was demonstrated to be highly effective (>99%) against the adult stages of C. oncophora, C. punctata, C. spatulata, C. surnabada, Cooperia spp. adult females and T. colubriformis adults.
authors.affiliation: Department of Veterinary Science, Louisiana State University, AgCenter, 111 Dalrymple Building, Baton Rouge, LA 70803-6002, USA. jcwilliams@agctr.lsu.edu
authors.forename: J C
authors.initials: JC
authors.lastname: Williams
authors.forename: A
authors.initials: A
authors.lastname: DeRosa
mesh_terms: D000287:Administration, Topical; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D000969:Antinematodal Agents / Q000008:administration & dosage*; D002417:Cattle; D002418:Cattle Diseases / Q000188:drug therapy* / Q000469:parasitology; D004305:Dose-Response Relationship, Drug; D007814:Larva / Q000187:drug effects; D018942:Macrolides; D008297:Male; D011897:Random Allocation; D014251:Trichostrongyloidea / Q000187:drug effects*; D014252:Trichostrongyloidiasis / Q000188:drug therapy / Q000662:veterinary*; D014254:Trichostrongylus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D000969:Antinematodal Agents; D018942:Macrolides; C027837:moxidectin