title: Amperometric biosensor with HRP immobilized on a sandwiched nano-Au / polymerized m-phenylenediamine film and ferrocene mediator.
abstract: An amperometric biosensor has been developed for the determination of H(2)O(2) in plant samples. Horseradish peroxidase (HRP) is immobilized on a sandwiched nano-Au particle / m-phenylenediamine polymer film by glutaraldehyde cross-linking. The film is formulated on the carbon paste electrode (CPE) blended with ferrocene as an electron transfer mediator. On the low concentration range, the current response is related to the H(2)O(2) concentration linearly from 0 to 8x10(-6) M with a detection limit of 1.3x10(-7) M. On a wider concentration range of 8x10(-6) to 1.4x10(-4) M, the reciprocal of current response is linearly related to the reciprocal of H(2)O(2) concentration. The apparent Michaelis-Menten constant (K(m)(app)) was calculated to be 0.0334 mM. The sensor has been tested by determining H(2)O(2) concentration in plant leaf samples.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, Chemistry and Chemical Engineering College, Department of Environmental Science and Engineering, Hunan University, 410082, Changsha, P.R. China.
authors.forename: Jin
authors.initials: J
authors.lastname: Li
authors.forename: Lang-Tao
authors.initials: LT
authors.lastname: Xiao
authors.forename: Xuan-Ming
authors.initials: XM
authors.lastname: Liu
authors.forename: Guang-Ming
authors.initials: GM
authors.lastname: Zeng
authors.forename: Guo-He
authors.initials: GH
authors.lastname: Huang
authors.forename: Guo-Li
authors.initials: GL
authors.lastname: Shen
authors.forename: Ru-Qin
authors.initials: RQ
authors.lastname: Yu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005296:Ferrous Compounds / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000032:analysis*; D000075163:Metallocenes; D036103:Nanotechnology; D010655:Phenylenediamines / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D012680:Sensitivity and Specificity
chemical_list: D004800:Enzymes, Immobilized; D005296:Ferrous Compounds; D000075163:Metallocenes; D010655:Phenylenediamines; D011108:Polymers; D006046:Gold; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; C008381:3-phenylenediamine; C004998:ferrocene