Transient expression of Arabidopsis thaliana ascorbate peroxidase 3 in Nicotiana benthamiana plants infected with recombinant potato virus X.
We have explored the transient over-expression of Arabidopsis thaliana ascorbate peroxidase 3 (APX3) in Nicotiana benthamiana using a viral vector based on the potato virus X (PVX). Plants infected with a PVX:APX3 hybrid had a similar progression of viral particles compared to control plants infected with a PVX:GFP hybrid, indicating that infection was not affected by the over-expression of heterologous APX3. Our results also showed that in PVX:APX3-infected plants, the hybrid virus directed a high level of APX3 expression and the recombinant protein was functional, as inferred from the higher APX activity compared to mock and PVX:GFP hybrid-infected plants. The PVX recombinant expression system used is a simple and quick method for transient expression of heterologous APXs, which are expected to suffer specific processing in plant cells.
Escobar
C
C
Departamento de Biología Vegetal, Facultad de Ciencias Ambientales, Universidad de Castilla la Mancha, Campus de la Fábrica de Armas, 45003, Toledo, Spain. cescobar@amb-to.uclm.es
Hernández
L E
LE
Jiménez
A
A
Creissen
G
G
Ruiz
M T
MT
Mullineaux
P M
PM
D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins; D060387:Ascorbate Peroxidases; D015152:Blotting, Northern; D060365:Chloroplast Proteins; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D005822:Genetic Vectors / Q000235:genetics; D015151:Immunoblotting; D008565:Membrane Proteins; D010544:Peroxidases / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D017863:Potexvirus / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics*
D029681:Arabidopsis Proteins; D060365:Chloroplast Proteins; D008565:Membrane Proteins; D018749:RNA, Plant; D010544:Peroxidases; C555294:APX3 protein, Arabidopsis; C555297:APXS protein, Arabidopsis; D060387:Ascorbate Peroxidases