Regional brain variations of cytochrome oxidase staining during olfactory learning in the honeybee (Apis mellifera).
Regional brain variations of cytochrome oxidase (CO) staining were analyzed in the honeybee (Apis mellifera) after olfactory conditioning of the proboscis extension reflex. Identification of brain sites where stimuli converge was done by precise image analysis performed in antennal lobes (AL) and mushroom bodies (MB). In Experiment 1, bees received 5 odorant stimulations that induced a transient decrease of CO activity in the lateral part of the AL. In Experiment 2, bees were trained with 5-trial olfactory conditioning. CO activity transiently increased in the lips of the MB calyces. There was also a delayed increase in the lateral part of the AL. An olfactory stimulus presented alone and an odor paired to a sucrose stimulation are treated by different pathways, including both AL and MB.
Déglise
Patrice
P
Laboratoire de Neurobiologie de l'Insecte, Université Paul Sabatier Toulouse III, France.
Dacher
Matthieu
M
Dion
Emmanuelle
E
Gauthier
Monique
M
Armengaud
Catherine
C
D000818:Animals; D001516:Bees / Q000737:chemistry* / Q000187:drug effects / Q000201:enzymology; D001923:Brain Chemistry / Q000187:drug effects / Q000502:physiology*; D003213:Conditioning, Psychological / Q000187:drug effects / Q000502:physiology; D003576:Electron Transport Complex IV / Q000032:analysis* / Q000378:metabolism; D007858:Learning / Q000187:drug effects / Q000502:physiology*; D012903:Smell / Q000187:drug effects / Q000502:physiology*; D013395:Sucrose / Q000494:pharmacology
D013395:Sucrose; D003576:Electron Transport Complex IV