Expression research for human DDX36 and mouse Ddx36 gene in the adult testis.
Drosophila melanogaster is one of the most intensively studied organisms in biology and serves as a model system for structural and functional genomic research. With the strategy of homologue molecular cloning using the sequence of the maleless gene (mle) of Drosophila, the novel homologous human and mouse genes with longer DNA/RNA helicase box (DEAD/DEAH box), named, DDX36 and Ddx36 genes, respectively, were cloned as new members of the DEAD/H box superfamily. In order to further investigate the relationship between those two genes of DDX36 and Ddx36 and the role of spermatogenesis, the expression analysis of them have been performed by the techniques of Northern blotting, RT-PCR and tissue in situ hybridization. The result indicated that the DDX36 and Ddx36 gene has highly expressed in the adult testis. It was primarily suggested that DDX36 and Ddx36 gene may be related with spermatogenesis.
Fu
Jun-Jiang
JJ
Laborary of Human Reproductive Engineering, Central South University Xiangya Medical College, Changsha 410078, China.
Li
Lu-Yun
LY
Liu
Shang-Feng
SF
Xing
Xiao-Wei
XW
Liu
Gang
G
Lu
Guang-Xiu
GX
D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D053487:DEAD-box RNA Helicases; D004265:DNA Helicases / Q000235:genetics*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D020365:RNA Helicases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000201:enzymology / Q000378:metabolism*
D018076:DNA, Complementary; D012333:RNA, Messenger; C466988:DHX36 protein, human; C466989:Dhx36 protein, mouse; D004265:DNA Helicases; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases