Evidence for endogenous interleukin-10 during nociception.
Cytokines such as IL-1beta, IL-6 and tumor necrosis factor-alpha (TNF-alpha) have been shown to contribute directly to central and peripheral neuropathic pain. Recently, exogenous interleukin-10 (IL-10) was shown to impede development of dynorphin-induced allodynia presumably by inhibiting IL-1beta. We therefore wanted to determine whether endogenous IL-10 had a role in pain perception. By measuring the latency of the paw licking response, we show in IL-10 knockout mice and in normal mice treated with anti-IL-10 that latency times are increased, suggesting that endogenous IL-10 increases nociception. This does not appear to be directly correlated with IL-10's regulation of DREAM, a transcriptional regulator of prodynorphin synthesis.
Tu
Huolin
H
Department of Microbiology and Immunology, University of Texas Medical Branch, Medical Research Building 4.174, Galveston, TX 77554-1019, USA.
Juelich
Terry
T
Smith
Eric M
EM
Tyring
Stephen K
SK
Rady
Peter L
PL
Hughes
Thomas K
TK
D000818:Animals; D000906:Antibodies; D002135:Calcium-Binding Proteins; D002460:Cell Line; D002490:Central Nervous System / Q000378:metabolism; D004399:Dynorphins / Q000378:metabolism; D016753:Interleukin-10 / Q000037:antagonists & inhibitors / Q000172:deficiency* / Q000235:genetics; D051605:Kv Channel-Interacting Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009437:Neuralgia / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D009619:Nociceptors / Q000378:metabolism*; D010147:Pain Measurement; D010523:Peripheral Nervous System Diseases / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D011930:Reaction Time / Q000235:genetics; D012097:Repressor Proteins / Q000378:metabolism
D000906:Antibodies; D002135:Calcium-Binding Proteins; C496756:Csen protein, mouse; D051605:Kv Channel-Interacting Proteins; D012097:Repressor Proteins; D016753:Interleukin-10; D004399:Dynorphins