title: Photocrosslinkable chitosan hydrogel containing fibroblast growth factor-2 stimulates wound healing in healing-impaired db/db mice.
abstract: Application of ultraviolet light (UV-) irradiation to a photocrosslinkable chitosan (Az-CH-LA) aqueous solution including fibroblast growth factor-2 (FGF-2) resulted within 30s in an insoluble, flexible hydrogel. About 20% of the FGF-2molecules were released from the FGF-2-incorporated chitosan hydrogel into phosphate buffered saline (PBS) within 1 day, after which no further significant release occurred under in vitro non-degradation conditions of the hydrogel. The FGF-2molecules retained in the chitosan hydrogel remained biologically active, and were released from the chitosan hydrogel upon the in vivo biodegradation of the hydrogel. In order to evaluate its accelerating effect on wound healing, full thickness skin incisions were made on the back of healing-impaired diabetic (db/db) mice and their normal (db/+) littermates. Application of the chitosan hydrogel significantly induced wound contraction and accelerated wound closure in both db/db and db/+ mice. However, the addition of FGF-2 in the chitosan hydrogel further accelerated wound closure in db/db mice, although not in db/+ mice. Histological examination also has demonstrated an advanced granulation tissue formation, capillary formation and epithelialization in wounds treated with FGF-2-incorporated chitosan hydrogels in db/db mice.
authors.affiliation: Department of Surgery II, National Defence Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan.
authors.forename: Kiyohaya
authors.initials: K
authors.lastname: Obara
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ishihara
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Ishizuka
authors.forename: Masanori
authors.initials: M
authors.lastname: Fujita
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Ozeki
authors.forename: Tadaaki
authors.initials: T
authors.lastname: Maehara
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Saito
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Yura
authors.forename: Takemi
authors.initials: T
authors.lastname: Matsui
authors.forename: Hidemi
authors.initials: H
authors.lastname: Hattori
authors.forename: Makoto
authors.initials: M
authors.lastname: Kikuchi
authors.forename: Akira
authors.initials: A
authors.lastname: Kurita
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002460:Cell Line; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry*; D048271:Chitosan; D004396:Coloring Agents / Q000378:metabolism; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D006801:Humans; D020100:Hydrogels; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D009779:Occlusive Dressings; D012867:Skin / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D014343:Trypan Blue / Q000378:metabolism; D014466:Ultraviolet Rays; D014945:Wound Healing / Q000502:physiology*
chemical_list: D001672:Biocompatible Materials; D004396:Coloring Agents; D020100:Hydrogels; D016222:Fibroblast Growth Factor 2; D002686:Chitin; D048271:Chitosan; D014343:Trypan Blue