Protection by L-fucose and fucose-rich polysaccharides against ROS-produced cell death in presence of ascorbate.
It was shown previously, that millimolar concentrations of ascorbate have cytotoxic and anti-proliferative effects (Eur. J. Clin. Invest. 32 (2002) 372). With increasing concentrations of ascorbate an increasing number of fibroblasts was detached from the culture dish and shown to be lysed by the effect of ascorbate-induced generation of reactive oxygen species (ROS-s). It also could be shown, that this cytotoxic effect is partly due to the dose-dependent inhibition by ascorbate of fibronectin biosynthesis. Superoxide dismutase (SOD) and catalase were shown to salvage cells from ROS-induced cell-death by preventing the inhibition of fibronectin biosynthesis. We used this model system to test the cyto-protective effect of L-fucose and fucose-rich oligo- and polysaccharides (FROP-s). It appeared that relatively low concentrations of L-fucose and FROP-3 (Biomed. Pharmacother. in press) could efficiently protect fibroblasts from the ascorbate-induced cell-death. These novel pharmacological properties of L-fucose and FROP-3 might well be related to their accelerating effect of wound healing.
Péterszegi
G
G
Laboratoire de Recherche en Ophtalmologie, Faculté de Médecine Broussais-Hôtel Dieu, Université Pierre et Marie Curie (Paris 6), 1, place Parvis Notre Dame, 75181 cedex 4, Paris, France.
Robert
A M
AM
Robert
L
L
D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D002374:Catalase / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology; D005353:Fibronectins / Q000096:biosynthesis; D005643:Fucose / Q000494:pharmacology*; D006801:Humans; D011134:Polysaccharides / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D012867:Skin / Q000166:cytology / Q000201:enzymology; D013482:Superoxide Dismutase / Q000494:pharmacology
D000975:Antioxidants; D005353:Fibronectins; D011134:Polysaccharides; D017382:Reactive Oxygen Species; D005643:Fucose; D002374:Catalase; D013482:Superoxide Dismutase; D001205:Ascorbic Acid