title: Visualization of an endophytic Streptomyces species in wheat seed.
abstract: Endophytic filamentous actinobacteria were isolated from surface-sterilized roots of wheat plants. Endophytic colonization of germinating wheat seed was examined using one of these endophytes, Streptomyces sp. strain EN27, tagged with the egfp gene. Endophytic colonization was observed from a very early stage of plant development with colonization of the embryo, endosperm, and emerging radicle.
authors.affiliation: Department of Medical Biotechnology, Flinders University, Bedford Park, South Australia 5042, Australia.
authors.forename: Justin T
authors.initials: JT
authors.lastname: Coombs
authors.forename: Christopher M M
authors.initials: CM
authors.lastname: Franco
mesh_terms: D018525:Germination / Q000502:physiology; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D018613:Microscopy, Confocal; D012639:Seeds / Q000254:growth & development / Q000382:microbiology*; D013302:Streptomyces / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure; D014908:Triticum / Q000254:growth & development / Q000382:microbiology*
chemical_list: D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins