Phosphoregulators: protein kinases and protein phosphatases of mouse.
With the completion of the human and mouse genome sequences, the task now turns to identifying their encoded transcripts and assigning gene function. In this study, we have undertaken a computational approach to identify and classify all of the protein kinases and phosphatases present in the mouse gene complement. A nonredundant set of these sequences was produced by mining Ensembl gene predictions and publicly available cDNA sequences with a panel of InterPro domains. This approach identified 561 candidate protein kinases and 162 candidate protein phosphatases. This cohort was then analyzed using TribeMCL protein sequence similarity clustering followed by CLUSTALV alignment and hierarchical tree generation. This approach allowed us to (1) distinguish between true members of the protein kinase and phosphatase families and enzymes of related biochemistry, (2) determine the structure of the families, and (3) suggest functions for previously uncharacterized members. The classifications obtained by this approach were in good agreement with previous schemes and allowed us to demonstrate domain associations with a number of clusters. Finally, we comment on the complementary nature of cDNA and genome-based gene detection and the impact of the FANTOM2 transcriptome project.
Forrest
Alistair R R
AR
The Institute for Molecular Bioscience, Australia. a.forrest@imb.uq.edu.au
Ravasi
Timothy
T
Taylor
Darrin
D
Huber
Thomas
T
Hume
David A
DA
Grimmond
Sean
S
D000818:Animals; D016000:Cluster Analysis; D019295:Computational Biology / Q000379:methods / Q000706:statistics & numerical data; D030541:Databases, Genetic / Q000706:statistics & numerical data; D006801:Humans; D051379:Mice; D010749:Phosphoprotein Phosphatases / Q000145:classification / Q000235:genetics*; D010766:Phosphorylation; D011237:Predictive Value of Tests; D011494:Protein Kinases / Q000145:classification / Q000235:genetics*; D017434:Protein Structure, Tertiary / Q000235:genetics
D011494:Protein Kinases; D010749:Phosphoprotein Phosphatases