Comparison of genotyping Helicobacter pylori directly from biopsy specimens and genotyping from bacterial cultures.
PCR for vacA and cagA genotypes of Helicobacter pylori using DNA isolated from infected gastric biopsy specimens was approximately equal to genotyping using bacterial DNA from cultures. Inconsistent results were associated with low H. pylori density in biopsies. A higher proportion of mixed infection was found when biopsies were used.
Park
Chang-Young
CY
Department of Medicine, Veterans Affairs Medical Center, and Baylor College of Medicine, Houston, Texas, USA.
Kwak
Minjung
M
Gutierrez
Oscar
O
Graham
David Y
DY
Yamaoka
Yoshio
Y
D000942:Antigens, Bacterial / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D001706:Biopsy; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis* / Q000302:isolation & purification; D005838:Genotype; D016481:Helicobacter Infections / Q000382:microbiology; D016480:Helicobacter pylori / Q000145:classification* / Q000235:genetics / Q000254:growth & development*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D013270:Stomach / Q000382:microbiology*
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D003470:Culture Media; D004269:DNA, Bacterial; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori