title: Epitope analysis of the MAb 1AD9 antibody detection site in human protein kinase CK2alpha-subunit.
abstract: 1AD9 is a murine monoclonal antibody (MAb) that was produced against the recombinant human alpha-subunit of protein kinase CK2, a pleiotropic and constitutively active serine/threonine protein kinase. We have further characterized this antibody, which is suitable for Western blot, immunoprecipitation and enzyme-linked immunosorbant assay tests. Using an overlapping peptide library, we have identified the epitope targeted by MAb1AD9 characterized by the following sequence: (319)MEHPYF(324).
authors.affiliation: Medizinische Biochemie und Molekularbiologie der Universitaet des Saarlandes, 66421 Homburg, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Nastainczyk
authors.forename: O-G
authors.initials: OG
authors.lastname: Issinger
authors.forename: B
authors.initials: B
authors.lastname: Guerra
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001666:Binding Sites, Antibody; D047390:Casein Kinase II; D020134:Catalytic Domain; D002417:Cattle; D002460:Cell Line; D018604:Epitope Mapping; D000939:Epitopes / Q000737:chemistry*; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000276:immunology*; D021122:Protein Subunits / Q000737:chemistry / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D021122:Protein Subunits; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases