Rapid staining and enumeration of small numbers of total bacteria in water by solid-phase laser cytometry.
The nucleic acid stain SYBR Green I was evaluated for use with solid-phase laser cytometry to obtain total bacterial cell counts from several water sources with small bacterial numbers. Results were obtained within 30 min and exceeded or equaled counts on R2A agar plates incubated for 14 days at room temperature.
Broadaway
Susan C
SC
Microbiology Department, Montana State University, Bozeman, MT 59717, USA.
Barton
Stephanie A
SA
Pyle
Barry H
BH
D001419:Bacteria / Q000302:isolation & purification*; D052160:Benzothiazoles; D015169:Colony Count, Microbial; D003959:Diamines; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D005456:Fluorescent Dyes / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D007834:Lasers; D008856:Microscopy, Fluorescence; D009930:Organic Chemicals; D011804:Quinolines; D013997:Time Factors; D014871:Water Microbiology
D052160:Benzothiazoles; D003959:Diamines; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I