Phase shifts and Per gene expression in mouse suprachiasmatic nucleus.
In mammals, circadian rhythms controlled by the suprachiasmatic nuclei are entrained by photic stimuli. To investigate the molecular mechanism of photic entrainment, we examined light-induced behavioral phase delays and associated changes in mPer1 and mPer2 gene expression in the suprachiasmatic nuclei of two mouse lines artificially selected for nest-building behavior. Big nest-builders show larger phase delays than small nest-builders. Light-induced mPer1 and mPer2 expression was examined in individual mice previously tested for phase shifting at circadian time 16. Light-induced mPer2 expression was significantly higher in big compared to small nest-builders. No difference was found between lines in light-induced mPer1 expression. The results suggest a more important role for mPer2 than for mPer1 gene expression in behavioral phase delays.
Yan
Lily
L
Department of Psychology, Columbia University, New York, NY 10027, USA.
Hochstetler
Kelly J
KJ
Silver
Rae
R
Bult-Ito
Abel
A
D000818:Animals; D018797:Cell Cycle Proteins; D005786:Gene Expression Regulation / Q000502:physiology*; D008297:Male; D051379:Mice; D009687:Nuclear Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D056950:Period Circadian Proteins; D010775:Photic Stimulation / Q000379:methods; D013493:Suprachiasmatic Nucleus / Q000737:chemistry / Q000378:metabolism*; D014157:Transcription Factors
D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; C539023:Per1 protein, mouse; C513312:Per2 protein, mouse; D056950:Period Circadian Proteins; D014157:Transcription Factors