title: Transcriptional program of the endocrine pancreas in mice and humans.
abstract: The Endocrine Pancreas Consortium was formed in late 1999 to derive and sequence cDNA libraries enriched for rare transcripts expressed in the mammalian endocrine pancreas. Over the past 3 years, the Consortium has generated 20 cDNA libraries from mouse and human pancreatic tissues and deposited >150,000 sequences into the public expressed sequence tag databases. A special effort was made to enrich for cDNAs from the endocrine pancreas by constructing libraries from isolated islets. In addition, we constructed a library in which fetal pancreas from Neurogenin 3 null mice, which consists of only exocrine and duct cells, was subtracted from fetal wild-type pancreas to enrich for the transcripts from the endocrine compartment. Sequence analysis showed that these clones cluster into 9,464 assembly groups (approximating unique transcripts) for the mouse and 13,910 for the human sequences. Of these, >4,300 were unique to Consortium libraries. We have assembled a core clone set containing one cDNA for each assembly group for the mouse and have constructed the corresponding microarray, termed "PancChip 4.0," which contains >9,000 nonredundant elements. We show that this PancChip is highly enriched for genes expressed in the endocrine pancreas. The mouse and human clone sets and corresponding arrays will be important resources for diabetes research.
authors.affiliation: Department of Genetics, University of Pennsylvania, Philadelphia 19104, USA. kaestner@mail.med.upenn.edu
authors.forename: Klaus H
authors.initials: KH
authors.lastname: Kaestner
authors.forename: Catherine S
authors.initials: CS
authors.lastname: Lee
authors.forename: L Marie
authors.initials: LM
authors.lastname: Scearce
authors.forename: John E
authors.initials: JE
authors.lastname: Brestelli
authors.forename: Athanasios
authors.initials: A
authors.lastname: Arsenlis
authors.forename: Phillip Phuc
authors.initials: PP
authors.lastname: Le
authors.forename: Kristen A
authors.initials: KA
authors.lastname: Lantz
authors.forename: Jonathan
authors.initials: J
authors.lastname: Crabtree
authors.forename: Angel
authors.initials: A
authors.lastname: Pizarro
authors.forename: Joan
authors.initials: J
authors.lastname: Mazzarelli
authors.forename: Deborah
authors.initials: D
authors.lastname: Pinney
authors.forename: Steve
authors.initials: S
authors.lastname: Fischer
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Manduchi
authors.forename: Christian J
authors.initials: CJ
authors.lastname: Stoeckert
authors.forename: Gerard
authors.initials: G
authors.lastname: Gradwohl
authors.forename: Sandra W
authors.initials: SW
authors.lastname: Clifton
authors.forename: Juliana R
authors.initials: JR
authors.lastname: Brown
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Inoue
authors.forename: Corentin
authors.initials: C
authors.lastname: Cras-Méneur
authors.forename: M Alan
authors.initials: MA
authors.lastname: Permutt
mesh_terms: D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D020224:Expressed Sequence Tags; D015723:Gene Library; D006801:Humans; D007515:Islets of Langerhans / Q000502:physiology*; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D011506:Proteins / Q000235:genetics; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; D011506:Proteins