title: Sensitive determination of pethidine in body fluids by gas chromatography-tandem mass spectrometry.
abstract: We have presented a simple and sensitive method for determining pethidine, a narcotic analgesic drug in body fluids by gas chromatography-tandem mass spectrometry (GC-MS/MS). Pethidine and 4'-piperidinoacetophenone (internal standard) were extracted from body fluids with Bond Elut C(18) columns; the recoveries were above 85% for both compounds. The calibration curves for blood and urine showed good linearities in the range of 1.25-40 ng/ml. Its detection limits (signal-to-noise ratio=3) were estimated to be approximately 0.5 ng/ml of whole blood and urine.
authors.affiliation: Department of Legal Medicine, Fujita Health University School of Medicine, 1-98 Kutsukake-cho, Toyoake 470-1192, Japan. akishii@fujita-hu.ac.jp
authors.forename: Akira
authors.initials: A
authors.lastname: Ishii
authors.forename: Miwa
authors.initials: M
authors.lastname: Tanaka
authors.forename: Rina
authors.initials: R
authors.lastname: Kurihara
authors.forename: Kanako
authors.initials: K
authors.lastname: Watanabe-Suzuki
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kumazawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Seno
authors.forename: Osamu
authors.initials: O
authors.lastname: Suzuki
authors.forename: Yoshinao
authors.initials: Y
authors.lastname: Katsumata
mesh_terms: D000701:Analgesics, Opioid / Q000097:blood / Q000378:metabolism* / Q000652:urine; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D008614:Meperidine / Q000097:blood / Q000378:metabolism* / Q000652:urine; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000701:Analgesics, Opioid; D008614:Meperidine