title: Structure of a conserved CoA-binding protein synthesized by a cell-free system.
abstract: TT1466 is a hypothetical protein from the extremely thermophilic bacterium Thermus thermophilus HB8 and is highly conserved in bacteria and archaea. The selenomethionyl protein was synthesized by a cell-free system and the crystal structure was determined at 2.0 A by MAD phasing. A native crystal was used for structure refinement to 1.7 A. The structure is highly homologous to that of the CoA-binding domain of the succinyl-CoA synthetase from Escherichia coli, despite the protein having only 14% sequence identity to this domain. An isothermal titration calorimetry experiment was performed to investigate whether TT1466 binds CoA and revealed high-affinity CoA binding of TT1466.
authors.affiliation: RIKEN Genomic Sciences Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Wada
authors.forename: Mikako
authors.initials: M
authors.lastname: Shirouzu
authors.forename: Takaho
authors.initials: T
authors.lastname: Terada
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Ishizuka
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Matsuda
authors.forename: Takanori
authors.initials: T
authors.lastname: Kigawa
authors.forename: Seiki
authors.initials: S
authors.lastname: Kuramitsu
authors.forename: Sam Yong
authors.initials: SY
authors.lastname: Park
authors.forename: Jeremy R H
authors.initials: JR
authors.lastname: Tame
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Yokoyama
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D002474:Cell-Free System / Q000378:metabolism; D003065:Coenzyme A / Q000737:chemistry*; D018360:Crystallography, X-Ray; D016415:Sequence Alignment; D016963:Thermus thermophilus / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D003065:Coenzyme A