[Construction of recombinant pEGFP/hTRT plasmid].
OBJECTIVE
To construct a plasmid which has a reporter gene for exploring the role of human telomerase reverse transcriptase(hTRT) in in-vitro cell cultivation.


METHODS
hTRT was cut by restricted enzyme from plasmid pGRN145 and inserted to plasmid pEGFP-C1 (enhanced green fluorescent protein).


RESULTS
Restricted enzyme analysis and DNA sequencing showed that the sequence of the pEGFP -hTRT transgenic plasmid was correct.


CONCLUSIONS
The recombinant vector pEGFP-hTRT has been successfully constructed, and it can be used as a transgenic plasmid in generating immortalized cell lines.
Zhou
Hai-wen
HW
Department of Oral Medicine, Ninth People's Hospital, Shanghai Second Medical University, Shanghai, P. R. China 200011.
Zhou
Zeng-tong
ZT
Yang
Wen-jun
WJ
Liu
De-li
DL
Li
Qing
Q
Hu
Hong-liang
HL
Liu
Wei
W
Cui
Lei
L
Cao
Yi-lin
YL
D004268:DNA-Binding Proteins; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics; D019098:Telomerase / Q000235:genetics*
D004268:DNA-Binding Proteins; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D019098:Telomerase