title: Crystallization of gankyrin, an oncoprotein that interacts with CDK4 and the S6b (rpt3) ATPase of the 19S regulator of the 26S proteasome.
abstract: Gankyrin is an oncoprotein overexpressed in hepatocarcinoma cells that binds to the cell-cycle regulator CDK4 and the S6b ATPase subunit of the regulatory component of the proteasome. It belongs to the family of ankyrin-repeat proteins that appear to mediate protein-protein interactions in diverse biochemical processes. Gankyrin has been crystallized from polyethylene glycol solutions and diffraction data have been obtained from these crystals that extend to 2.1 A spacing.
authors.affiliation: Department of Chemistry, University of York, York YO10 5YW, England.
authors.forename: Szymon
authors.initials: S
authors.lastname: Krzywda
authors.forename: Andrzej M
authors.initials: AM
authors.lastname: Brzozowski
authors.forename: Roisin
authors.initials: R
authors.lastname: Al-Safty
authors.forename: Rebecca
authors.initials: R
authors.lastname: Welchman
authors.forename: Maureen
authors.initials: M
authors.lastname: Mee
authors.forename: Simon
authors.initials: S
authors.lastname: Dawson
authors.forename: Jun
authors.initials: J
authors.lastname: Fujita
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Higashitsuji
authors.forename: R John
authors.initials: RJ
authors.lastname: Mayer
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Wilkinson
mesh_terms: D003460:Crystallization / Q000379:methods; D006801:Humans; D015513:Oncogene Proteins / Q000737:chemistry*; D011092:Polyethylene Glycols; D046988:Proteasome Endopeptidase Complex; D011518:Proto-Oncogene Proteins; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: D015513:Oncogene Proteins; C403591:PSMD10 protein, human; D011518:Proto-Oncogene Proteins; D011092:Polyethylene Glycols; D046988:Proteasome Endopeptidase Complex