Transglucosylation activities of multiple forms of alpha-glucosidase from spinach.
Transglucosylation activities of spinach alpha-glucosidase I and IV, which have different substrate specificity for hydrolyzing activity, were investigated. In a maltose mixture, alpha-glucosidase I, which has high activity toward not only maltooligosaccharides but also soluble starch and can hydrolyze isomaltose, produced maltotriose, isomaltose, and panose, and alpha-glucosidase IV, which has high activity toward maltooligosaccharides but faint activity toward soluble starch and isomaltose, produced maltotriose, kojibiose, and 2,4-di-alpha-D-glucosyl-glucose. Transglucosylation to sucrose by alpha-glucosidase I and IV resulted in the production of theanderose and erlose, respectively, showing that spinach alpha-glucosidase I and IV are useful to synthesize the alpha-1,6-glucosylated and alpha-1,2- and 1,4-glucosylated products, respectively.
Sugimoto
Manabu
M
Research Institute for Bioresources, Okayama University, Kurashiki, Okayama 710-0046, Japan. manabus@rib.okayama-u.ac.jp
Furui
Satoshi
S
Sasaki
Kenji
K
Suzuki
Yukio
Y
D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005936:Glucans / Q000378:metabolism; D006031:Glycosylation; D007527:Isoenzymes / Q000378:metabolism; D007534:Isomaltose / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000378:metabolism; D018724:Spinacia oleracea / Q000201:enzymology*; D013213:Starch / Q000378:metabolism; D013379:Substrate Specificity; D000520:alpha-Glucosidases / Q000378:metabolism*
D005936:Glucans; D007527:Isoenzymes; D009844:Oligosaccharides; C008763:panose; D007534:Isomaltose; D013213:Starch; D000520:alpha-Glucosidases