Xenoreactive anti-Galalpha(1,3)Gal antibodies prevent porcine endogenous retrovirus infection of human in vivo.
The discovery of porcine endogenous retroviruses (PERV) has raised concerns regarding the safety of pig to human xenotransplantation. In this study, we examined PERV infection of human cells in vivo. Furthermore, we examined the effect of human xenoreactive natural antibody on in vivo PERV infection. Human peripheral blood leukocyte reconstituted severe combined immunodeficiency mice were transplanted with porcine aortic endothelial cells (PAEC). PERV gene expression was readily detected in human leukocytes after transplantation. In contrast, human leukocytes harvested from mice treated with human serum or anti-Galalpha(1,3)Gal antibody prevented PERV infection in 6 of 8 mice. These results provide the first evidence that PERV can infect human cells in vivo and that natural xenoreactive antibody can prevent this infection.
McKane
Brice W
BW
Department of Surgery, Washington University School of Medicine, St. Louis, MO 63110, USA.
Ramachandran
Sabarinathan
S
Yang
Junbao
J
Xu
Xiao-Chun
XC
Mohanakumar
T
T
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D002460:Cell Line; D017690:Cell Transplantation; D002478:Cells, Cultured; D018920:Coculture Techniques; D004187:Disaccharides / Q000276:immunology*; D020077:Endogenous Retroviruses / Q000235:genetics / Q000502:physiology*; D004730:Endothelium, Vascular / Q000166:cytology* / Q000821:virology; D005434:Flow Cytometry; D015870:Gene Expression; D006801:Humans; D007962:Leukocytes / Q000821:virology; D051379:Mice; D016513:Mice, SCID; D012367:RNA, Viral / Q000032:analysis; D012192:Retroviridae Infections / Q000276:immunology / Q000517:prevention & control* / Q000635:transmission / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine / Q000821:virology*; D014183:Transplantation, Heterologous
D000906:Antibodies; D004187:Disaccharides; D012367:RNA, Viral; C055075:galactosyl-(1-3)galactose