title: Cross-reactivity of the PLATELIA CANDIDA antigen detection enzyme immunoassay with fungal antigen extracts.
abstract: We studied the specificity of the PLATELIA CANDIDA Ag enzyme immunoassay by using 130 isolates of 63 clinically relevant fungal species. Antigen extracts of seven Candida spp. (Candida albicans, C. dubliniensis, C. famata, C. glabrata, C. guilliermondii, C. lusitaniae, and C. tropicalis) repeatedly yielded positive reactions (>0.5 ng/ml). Geotrichum candidum and Fusarium verticillioides were found to yield borderline-positive reactions (0.25 to 0.50 ng/ml). Antigen preparations from the other 54 fungal species, including yeasts, molds, dermatophytes, and dimorphic fungi, did not cross-react in the assay.
authors.affiliation: Department of Medical Microbiology and Hospital Hygiene, University Hospital, D-18057 Rostock, Germany. drimek@tllv.thueringen.de
authors.forename: Dagmar
authors.initials: D
authors.lastname: Rimek
authors.forename: Jagpal
authors.initials: J
authors.lastname: Singh
authors.forename: Reinhard
authors.initials: R
authors.lastname: Kappe
mesh_terms: D000946:Antigens, Fungal / Q000276:immunology*; D002175:Candida / Q000145:classification / Q000276:immunology*; D002177:Candidiasis / Q000382:microbiology; D003429:Cross Reactions; D005658:Fungi / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D009181:Mycoses / Q000382:microbiology; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results
chemical_list: D000946:Antigens, Fungal; D011933:Reagent Kits, Diagnostic