title: Main flavonoids in the root of Scutellaria baicalensis cultivated in Europe and their comparative antiradical properties.
abstract: The content of the main flavonoids in the root of Scutellaria baicalensis Georgi cultivated in Central Europe was evaluated using the new simple RP-HPLC method with gradient of acetonitrile in mobile phase. The main components of the roots were baicalin (8.12% of dry root mass) and wogonin glucuronide (2.52%). The content of flavonoids was comparable with the content in plants cultivated in natural localities. Five main flavonoids were evaluated for their scavenging ability with DPPH radical-generating system and due to limited solubility only two flavonoids were investigated for their ability to scavenge hydroxyl radical by the aromatic hydroxylation method. The total extract was also tested in both the experimental arrangements. In experiments with DPPH, only baicalin and baicalein displayed a significant scavenging effect, while the production of OH radicals generated by UV photolysis of H(2)O(2) was considerable decreased in the presence of baicalin and wogonin glucuronide. After comparison with results obtained for the total extract, it was concluded, that the scavenging activity of the extract against DPPH is mainly derived from baicalin. On the other hand, baicalin, wogonin glucuronide and probably other flavonoids participate in scavenging OH radical.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Masaryk University in Brno, Brno, Czech Republic.
authors.forename: Hana
authors.initials: H
authors.lastname: Bochoráková
authors.forename: Hana
authors.initials: H
authors.lastname: Paulová
authors.forename: Jirí
authors.initials: J
authors.lastname: Slanina
authors.forename: Pavel
authors.initials: P
authors.lastname: Musil
authors.forename: Eva
authors.initials: E
authors.lastname: Táborská
mesh_terms: D001713:Biphenyl Compounds; D002851:Chromatography, High Pressure Liquid; D005060:Europe; D044950:Flavanones; D005419:Flavonoids / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D016166:Free Radical Scavengers / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D020719:Glucuronides / Q000737:chemistry; D006801:Humans; D017665:Hydroxyl Radical; D020128:Inhibitory Concentration 50; D008517:Phytotherapy; D010851:Picrates; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D018517:Plant Roots; D031364:Scutellaria baicalensis
chemical_list: D001713:Biphenyl Compounds; D044950:Flavanones; D005419:Flavonoids; D016166:Free Radical Scavengers; D020719:Glucuronides; D010851:Picrates; D010936:Plant Extracts; C465289:wogonin-7-glucuronide; D017665:Hydroxyl Radical; C038044:baicalin; C004931:1,1-diphenyl-2-picrylhydrazyl