title: Reduction of mercuric ion in vitro by superoxide anion.
abstract: The reduction rate of mercuric ion to metallic mercury by a superoxide anion produced by a xanthine-xanthine oxidase system increased with an increased concentration of xanthine oxidase in the presence of enough xanthine. The reduction rate of mercuric ion by a superoxide anion in the presence of nitroblue tetrazolium (NBT) was proportional to the concentration of NBT. The result suggests that NBT was reduced to diformazan by a superoxide anion produced by a xanthine-xanthine oxidase system and that mercuric ion will be reduced to metallic mercury by diformazan. The reduction rate of mercuric ion was also indicative that a superoxide anion produced by an NADH-phenazine methosulfate (PMS) system increased with an increased concentration of PMS.
authors.affiliation: Department of Chemistry, Faculty of Science, Okayama University of Science, 1-1 Ridai-cho, Okayama City 700-0005, Japan.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Aikoh
mesh_terms: D005029:Ethylenediamines / Q000737:chemistry; D007477:Ions / Q000737:chemistry; D008628:Mercury / Q000737:chemistry*; D008773:Methylphenazonium Methosulfate / Q000737:chemistry; D009097:Multienzyme Complexes / Q000737:chemistry; D009243:NAD / Q000737:chemistry*; D009580:Nitroblue Tetrazolium / Q000737:chemistry; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D012256:Riboflavin; D012680:Sensitivity and Specificity; D013481:Superoxides / Q000138:chemical synthesis / Q000737:chemistry*; D019820:Xanthine / Q000737:chemistry; D014969:Xanthine Oxidase / Q000737:chemistry*
chemical_list: D005029:Ethylenediamines; D007477:Ions; D009097:Multienzyme Complexes; D009243:NAD; D013481:Superoxides; D019820:Xanthine; D009580:Nitroblue Tetrazolium; D008773:Methylphenazonium Methosulfate; C031234:ethylenediamine; D014969:Xanthine Oxidase; D008628:Mercury; D012256:Riboflavin