[Specific loss of heterozygosity of chromosome 3p loci in soft tissue leiomyosarcoma].
OBJECTIVE
To analyze the loss of heterozygosity (LOH) at 5 loci on chromosome 3p in soft tissue leiomyosarcoma (LMS).


METHODS
LOH was detected in 22 cases of LMS using PCR-silver staining targeting 5 microsatellite sites on 3p14.2-pter. Relation between LOH and LMS clinical pathological features was also analyzed.


RESULTS
Ten of 22 LMS samples showed LOH at more than one locus (45.4%). Among the 5 loci, LOH occurred more frequently at D3s1295 (36.8%) and D3s1289 (10.5%), but absent at D3s1293. No significant difference was found on LOH incidence between different grade, size and location of LMS.


CONCLUSIONS
LOH on chromosome 3p14.2-23 region is relatively frequent in LMS. Region around D3s1295 and D3s1289 may harbor tumor suppressor gene relating to LMS.
Peng
Hui
H
Department of Pathology, Huaxi Hospital, Sichuan University, Chengdu 610041, China.
Yang
Guang-hua
GH
Bu
Hong
H
Gou
Li-xin
LX
D000008:Abdominal Neoplasms / Q000235:genetics / Q000473:pathology; D002893:Chromosomes, Human, Pair 3; D005121:Extremities; D016147:Genes, Tumor Suppressor; D006801:Humans; D007890:Leiomyosarcoma / Q000235:genetics* / Q000473:pathology; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats; D012186:Retroperitoneal Neoplasms / Q000235:genetics / Q000473:pathology; D012983:Soft Tissue Neoplasms / Q000235:genetics* / Q000473:pathology