The promoter of an antifungal protein gene from Gastrodia elata confers tissue -specific and fungus-inducible expression patterns and responds to both salicylic acid and jasmonic acid.
Gastrodia antifungal proteins (GAFPs) are a group of mannose-binding lectins purified from Gastrodia elata that show strong resistance against a wide spectrum of fungi. The GAFP-2 promoter was analyzed for its ability to control the expression of the reporter gene, beta-glucuronidase (GUS) in transgenic tobacco plants. The GUS assays revealed that the GAFP-2 promoter is expressed in a tissue-specific manner, which mainly expressed in the vascular cells. The highest GUS activity was observed in roots, followed by stems. GAFP-2-GUS expression was strongly induced by the fungus Trichoderma viride and by the plant stress regulators, salicylic acid and jasmonic acid in the stably transformed tobacco plants. The -537 region of the GAFP-2 promoter was sufficient for its tissue-specific and inducible expression of the promoter.
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Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Building 917, 100101 Beijing, P. R. China.
Wang
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Li
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Zhang
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Sun
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Y
D003517:Cyclopentanes / Q000494:pharmacology*; D031668:Gastrodia / Q000187:drug effects / Q000235:genetics* / Q000382:microbiology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D054883:Oxylipins; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics*; D020156:Salicylic Acid / Q000494:pharmacology*; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics / Q000382:microbiology; D014242:Trichoderma / Q000254:growth & development
D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; C011006:jasmonic acid; D005966:Glucuronidase; D020156:Salicylic Acid