[Significance of monitoring hematopoietic progenitor cells in peripheral blood stem cell during mobilization and harvest].
To achieve efficient peripheral blood stem cell harvest (PBSCH), a simple method to monitor peripheral blood stem/progenitor cells was evaluated. The Sysmex XE-2100 hematology analyzer with an immature information (IMI) channel was used to identify and count the hematopoietic progenitor cell (HPC). Twenty-five donors mobilized with G-CSF in allogeneic and 11 patients in autologous peripheral blood stem cell transplantation (allo-PBSCT and auto-PBSCT) were involved. The HPC, CD34(+) cell and CFU-GM in the peripheral blood and leukapheresis samples were detected during mobilization and harvest. The results showed that HPC amount had a positive correlation with both the CD34(+) cell and CFU-GM in the peripheral blood. The peripheral blood hematopoietic stem/progenitor cells in allo-PBSCT donors remarkably increased on day 5 of the mobilization, followed the leukocytes increased. However, a fast increase of hematopoietic stem/progenitor cells was earlier than leukocytes in the peripheral blood. The HPC positively correlated with the CD34(+) cell or CFU-GM in the PBSCH. On the days of collection, the count of HPC and CD34(+) cell in peripheral blood was highly correlated with the CD34(+) cell yield. It is concluded that HPC as an estimate of progenitor cells in collected blood sample could be used to determine the optimal time of PBSCH and minimize the risk of missing an adequate harvest.
Yang
Shen-Miao
SM
Institute of Hematology and People's Hospital, Peking University, Beijing 100044, China.
Liu
Kai-Yan
KY
Lu
Dao-Pei
DP
D018952:Antigens, CD34 / Q000097:blood; D001782:Blood Donors; D002469:Cell Separation / Q000379:methods*; D003114:Colony-Forming Units Assay; D006405:Hematology / Q000295:instrumentation / Q000379:methods*; D019650:Hematopoietic Stem Cell Mobilization; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007962:Leukocytes / Q000166:cytology / Q000276:immunology
D018952:Antigens, CD34