Unique scanning capabilities of a new hybrid linear ion trap mass spectrometer (Q TRAP) used for high sensitivity proteomics applications.
The unique scanning capabilities of a hybrid linear ion trap (Q TRAP) mass spectrometer are described with an emphasis on proteomics applications. The combination of the very selective triple quadrupole based tandem mass spectrometry (MS/MS) scans with the very sensitive ion trap product ion scans allows rapid identification of peptides at low concentrations derived from post-translationally modified proteins on chromatographic time scales. The Q TRAP instrument also offers the opportunity to conduct a variety of ion processing steps prior to performing a mass scan. For example, the enhancement of the multiple-charge ion contents of the ion trap can be performed resulting in a survey mass spectrum dominated by double- and triple-charge peptides. This facilitates the identification of relevant biological species in both separated and unseparated peptide mixtures for further MS/MS experiments.
Le Blanc
J C Yves
JC
MDS Sciex, Concord (Ont), Canada.
Hager
James W
JW
Ilisiu
A M Patricia
AM
Hunter
Christie
C
Zhong
Feng
F
Chu
Ivan
I
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry / Q000295:instrumentation*; D010748:Phosphopeptides / Q000032:analysis*; D020543:Proteome; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000032:analysis
D010748:Phosphopeptides; D020543:Proteome; D012710:Serum Albumin, Bovine