[Construction of eukaryotic expression vector for rat myogenin gene].
OBJECTIVE
To construct eukaryotic expression vector of rat myogenin gene for further study on its functions in skeletal muscle denervated atrophy and repair.


METHODS
The cloning vectors (containing full length of myogenin cDNA and two restriction sites: Hind III and Xho I) were first cut by two restriction endonuclease: Hind III and Xho I, and the same as the eukaryotic expression vector; then, the myogenin cDNA and the digested vector were ligated by T4 DNA ligase, and recombinant eukaryotic expression vector was formed. Its length was certificated by agarose gel electrophoresis analysis, digestion with Hind III and Xho I, PCR; and the rightness of the myogenin cDNA sequence was confirmed by sequencing.


RESULTS
The results of agarose gel electrophoresis analysis, digestion, and PCR confirmed the right length of inserted DNA, which was the same as the myogenin cDNA, and the sequencing result of pcDNA3-myogenin was identical with the reported.


CONCLUSIONS
pcDNA3-myogenin a eukaryotic expression vector, is successfully constructed.
Jiang
Hao
H
Department of Hand Surgery, Huashan Hospital, Fudan University, Shanghai, P.R. China 200040.
Xu
Jian-guang
JG
Gu
Yu-dong
YD
Hu
Shao-nan
SN
Li
Ji-feng
JF
D000818:Animals; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000235:genetics; D005057:Eukaryotic Cells / Q000378:metabolism; D015870:Gene Expression; D005822:Genetic Vectors; D018008:Myogenin / Q000096:biosynthesis / Q000235:genetics*; D051381:Rats
D018076:DNA, Complementary; C503803:Myog protein, rat; D018008:Myogenin