Impairment of the renal p-aminohippurate transport induced by 6-hydroxydopamine.
In this study, the effects of 6-hydroxydopamine (6-OHDA) on renal p-aminohippurate transport were investigated in-vitro using rat renal cortical slices. Cisplatin, a known nephrotoxin, was used as positive control. Renal cortical slices were incubated for 60 min in a cisplatin-containing medium (0.83-5.0 microM) at 37 degrees C under a 100% O(2) atmosphere. In another series of experiments, renal cortical slices were incubated in a 3.33 microM cisplatin-containing medium for 15-120 min or in a cisplatin-free medium. Subsequently, for each series of experiments, kidney slices were incubated at 25 degrees C for 90 min in a media containing p-aminohippurate. In a further series of experiments, renal cortical slices were incubated for 60 min in a 6-OHDA containing medium (3.125-100 microM) at 37 degrees C under a 100% O(2) atmosphere. In another series of experiments, renal cortical slices were incubated in a 50 microM 6-OHDA-containing medium for 15-120 min or in 6-OHDA-free medium. Subsequently, for each series of experiments, kidney slices were incubated at 25 degrees C for 90 min in a media containing p-aminohippurate. The results of this study where slices were incubated in 6-OHDA- or cisplatin-containing media indicate that both 6-OHDA and cisplatin induced a time- and concentration-dependent decrease in p-aminohippurate accumulation by renal cortical slices. Therefore, similarly to cisplatin, 6-OHDA causes functional injury of renal proximal tubule cells, leading to impairment of transport processes across the cell membrane.
Cojocel
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Faculty of Medicine, Department of Pharmacology and Toxicology, Kuwait University, Safat, Kuwait. cojocel@hsc.kuniv.edu.kw
Thomson
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MS
D000818:Animals; D001692:Biological Transport / Q000187:drug effects / Q000502:physiology; D002945:Cisplatin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D016627:Oxidopamine / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D010130:p-Aminohippuric Acid / Q000037:antagonists & inhibitors / Q000378:metabolism*
D016627:Oxidopamine; D002945:Cisplatin; D010130:p-Aminohippuric Acid