title: Genomic characterisation of putative growth hormone releasing hormone (GHRH) receptor genes in the teleost fish Fugu rubripes.
abstract: The genomic organisation of a putative GHRH receptor gene has been characterised in the teleost fish, Fugu rubripes. It comprises 13 exons and is at least 1.2 x larger than previously described vertebrate GHRH receptors. Sequence conservation with other vertebrate GHRH receptors is highest over the seven transmembrane domains with preservation of 8 conserved cysteines and an N-glycosylation site in the N-terminal region, which are important for receptor activation. Database mining revealed a further putative GHRH receptor in Fugu, with phylogenetic tree topology indicating that this was potentially a teleost-specific duplication event. This is the first time that the genomic organisation of a GHRH receptor gene has been characterised from a nonmammalian vertebrate.
authors.affiliation: Fugu Genomics Group, MRC-HGMP Resource Centre, Genome Campus, Hinxton, Cambridge, UK. jcardosa@hgmp.mrc.ac.uk
authors.forename: João C
authors.initials: JC
authors.lastname: Cardoso
authors.forename: Deborah M
authors.initials: DM
authors.lastname: Power
authors.forename: Greg
authors.initials: G
authors.lastname: Elgar
authors.forename: Melody S
authors.initials: MS
authors.lastname: Clark
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D003360:Cosmids; D003545:Cysteine / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D005091:Exons; D015723:Gene Library; D008040:Genetic Linkage; D006031:Glycosylation; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D018013:Receptors, Neuropeptide / Q000235:genetics*; D018017:Receptors, Pituitary Hormone-Regulating Hormone / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D023741:Takifugu / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D018013:Receptors, Neuropeptide; D018017:Receptors, Pituitary Hormone-Regulating Hormone; C048250:somatotropin releasing hormone receptor; D003545:Cysteine