title: Development of ribozyme synthesis system using a rolling-synchronization: effect of template DNA secondary structure on recognition of RNA polymerase.
abstract: Circular DNA behaves as an efficient template for RNA polymerase (RNAP) in rolling circle transcription (RCT). We found that the short hairpin-stem structure in the circular DNA without promoter enhances the DNA recognition of T7 RNAP in RCT. The result indicates that T7 RNAP would distinguish the short DNA hairpin-stem structure.
authors.affiliation: High Technology Research Center, Faculty of Science and Engineering, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ohmichi
authors.forename: M
authors.initials: M
authors.lastname: Nakamura
authors.forename: N
authors.initials: N
authors.lastname: Sugimoto
mesh_terms: D001483:Base Sequence; D004270:DNA, Circular / Q000737:chemistry*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016337:RNA, Catalytic / Q000096:biosynthesis* / Q000737:chemistry; D013698:Templates, Genetic; D014158:Transcription, Genetic
chemical_list: D004270:DNA, Circular; D016337:RNA, Catalytic; D012321:DNA-Directed RNA Polymerases