Specific interaction of PML bodies with the TP53 locus in Jurkat interphase nuclei.
PML bodies play an important role in multiple pathways of growth control, such as transformation suppression, apoptosis, and Ras-induced senescence. However, the molecular and biological bases for these physiological phenomena are not well understood. The findings that viruses transcribe their genomes adjacent to PML bodies and that nascent RNA accumulates at their periphery have suggested that PML bodies are transcription-permissible domains. To investigate the role of PML bodies in regulation of cell transformation and apoptosis-related gene transcription, we employed the immuno-FISH method to examine the relationship between PML bodies and the TP53 and BCL2 gene loci. PML bodies were found to localize specifically with the TP53 locus in about 50% of Jurkat interphase nuclei, but never in proximity with the BCL2 locus. We speculate that PML bodies may interact directly with the TP53 DNA sequence to regulate TP53 gene expression.
Sun
Yujie
Y
Division of Molecular Medicine, Beckman Research Institute of City of Hope National Medical Center, 1500 E. Duarte Road, Duarte, CA 91010, USA.
Durrin
Linda K
LK
Krontiris
Theodore G
TG
D017209:Apoptosis / Q000235:genetics; D002472:Cell Transformation, Viral / Q000235:genetics; D015870:Gene Expression; D019254:Genes, bcl-2 / Q000235:genetics*; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D019169:Jurkat Cells; D009363:Neoplasm Proteins / Q000235:genetics*; D009687:Nuclear Proteins; D000071156:Promyelocytic Leukemia Protein; D014157:Transcription Factors / Q000235:genetics*; D025521:Tumor Suppressor Proteins
D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D000071156:Promyelocytic Leukemia Protein; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; C070576:PML protein, human