title: Efficient synthesis of globoside and isogloboside tetrasaccharides by using beta(1-->3) N-acetylgalactosaminyltransferase/UDP-N-acetylglucosamine C4 epimerase fusion protein.
abstract: The beta(1-->3) N-acetylgalactosaminyltransferase/UDP-N-acetylglucosamine C4 epimerase fusion protein was constructed and used in coupled enzymatic reactions to synthesize a variety of globotetraose and isoglobotetraose derivatives from the corresponding lactoside acceptors.
authors.affiliation: Department of Chemistry, Wayne State University, Detroit, MI 48202, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Shao
authors.forename: Jianbo
authors.initials: J
authors.lastname: Zhang
authors.forename: Przemyslaw
authors.initials: P
authors.lastname: Kowal
authors.forename: Yuquan
authors.initials: Y
authors.lastname: Lu
authors.forename: Peng George
authors.initials: PG
authors.lastname: Wang
mesh_terms: D002240:Carbohydrate Sequence; D005915:Globosides / Q000138:chemical synthesis*; D006027:Glycosides / Q000737:chemistry; D008956:Models, Chemical; D008969:Molecular Sequence Data; D017350:N-Acetylgalactosaminyltransferases / Q000737:chemistry*; D009844:Oligosaccharides / Q000138:chemical synthesis*; D004364:Pharmaceutical Preparations; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D014534:UDPglucose 4-Epimerase / Q000737:chemistry*; D014531:Uridine Diphosphate Galactose / Q000737:chemistry
chemical_list: D005915:Globosides; D006027:Glycosides; D009844:Oligosaccharides; D004364:Pharmaceutical Preparations; D011993:Recombinant Fusion Proteins; C008245:lactosides; D014531:Uridine Diphosphate Galactose; C035968:globotetraose; D017350:N-Acetylgalactosaminyltransferases; D014534:UDPglucose 4-Epimerase