title: Immunohistochemical analysis of two strains of lion (Panthera leo)-adapted canine distemper virus in ferrets (Mustela putorius furo).
abstract: Canine distemper virus (CDV) caused epizootics in lions (Panthera leo) in Tanzania's Serengeti National Park in 1994 and in captive lions and other Panthera spp. in the USA in 1991-1992. In this study, immunohistochemistry was used to compare viral distribution in tissues collected from ferrets (Mustela putorius furo) inoculated with one of the two lion-derived CDV isolates, either from Serengeti (A94-11/13) or from California (A92-27/20). The California isolate resulted in severe morbidity in all nine ferrets, whereas the Serengeti isolate resulted in severe morbidity in five of the nine ferrets. A slightly higher proportion of infected cells was found in many tissues in the Serengeti isolate-inoculated ferrets. These findings indicate that the pathogenicity of the California isolate is not directly related to the number of infected cells.
authors.affiliation: Department of Veterinary Microbiology and Pathology, Washington State University, PO Box 647040, Pullman, WA 99164-7040, USA. jstanton@vetmed.wsu.edu
authors.forename: J B
authors.initials: JB
authors.lastname: Stanton
authors.forename: L
authors.initials: L
authors.lastname: Givens
authors.forename: J F
authors.initials: JF
authors.lastname: Evermann
authors.forename: C C
authors.initials: CC
authors.lastname: Brown
mesh_terms: D000818:Animals; D001652:Bile Ducts / Q000821:virology; D001921:Brain / Q000821:virology; D004216:Distemper / Q000821:virology; D004217:Distemper Virus, Canine / Q000145:classification* / Q000302:isolation & purification*; D004848:Epithelium / Q000821:virology; D005289:Ferrets / Q000821:virology*; D007150:Immunohistochemistry; D008045:Lions / Q000821:virology*; D008221:Lymphoid Tissue / Q000821:virology; D012137:Respiratory System / Q000821:virology; D012469:Salivary Glands / Q000821:virology; D012867:Skin / Q000821:virology