NMR solution structure of the 2.1 loop of Tetrahymena group I intron.
The solution structure of 17 mer RNA contained of the 2.1 loop region of Tetrahymena group I intron was studied by NMR. This RNA oligomer forms a hairpin structure at low NaCl concentration (5 mM). In the hairpin structure, the bases from C1 to A8 are sequentially stacked, and one GC base pair is formed in the loop. By the addition of RNA oligomer GCAA compensatory to UUGC in the loop, the imino proton signals of one AU and one GC base pairs are observed. Therefore, tetramer GCAA interacts with the loop of the hairpin structure. The 2.1 loop region is presumed to interact with the 9.1a loop.
Amano
M
M
Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565, Japan.
D000818:Animals; D001483:Base Sequence; D007438:Introns; D019906:Nuclear Magnetic Resonance, Biomolecular; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry; D013768:Tetrahymena / Q000235:genetics*
D012313:RNA