title: Truncated forms of human and simian immunodeficiency virus in infected individuals and rhesus macaques are unique or rare quasispecies.
abstract: Truncated proviruses of variable sizes are present in peripheral blood mononuclear cells (PBMC) of human immunodeficiency virus type 1 (HIV-1)-infected persons and simian immunodeficiency virus (SIV)-infected rhesus macaques. Here, we investigated whether the highly deleted HIV and SIV proviruses are present in infected organisms as multiple copies or whether each truncated provirus is unique. Using end-point dilution, multiple long-distance (LD) DNA PCR assays were run in parallel using DNA extracted from PBMC of seropositive, treatment-naive persons and from lymph nodes of a rhesus monkey inoculated with cloned, full-length SIVmac239 DNA. The PCR products were titrated and mapped. Most truncated proviruses were present in the DNA samples tested as single, nonintegrated molecules that differed from one another in size and/or nucleotide sequence. These results indicate that truncated primate lentiviral sequences found in infected tissues are unique or rare quasispecies that do not replicate significantly.
authors.affiliation: INSERM U372, Unité de Pathogénie des Infections à Lentivirus, Parc Scientifique et Technologique de Luminy, 13273, Marseille, France.
authors.forename: Marjorie
authors.initials: M
authors.lastname: Pion
authors.forename: Giselle
authors.initials: G
authors.lastname: Sanchez
authors.forename: Vladimir
authors.initials: V
authors.lastname: Liska
authors.forename: Lise
authors.initials: L
authors.lastname: Bettendroffer
authors.forename: Daniel
authors.initials: D
authors.lastname: Candotti
authors.forename: Agnès Laurence
authors.initials: AL
authors.lastname: Chenine
authors.forename: Françoise
authors.initials: F
authors.lastname: Gondois-Rey
authors.forename: Catherine
authors.initials: C
authors.lastname: Tamalet
authors.forename: Robert
authors.initials: R
authors.lastname: Vigne
authors.forename: Ruth M
authors.initials: RM
authors.lastname: Ruprecht
authors.forename: Henri
authors.initials: H
authors.lastname: Agut
authors.forename: Ivan
authors.initials: I
authors.lastname: Hirsch
mesh_terms: D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D017353:Gene Deletion; D014644:Genetic Variation; D006679:HIV Seropositivity / Q000097:blood / Q000821:virology*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology; D008198:Lymph Nodes / Q000821:virology; D008253:Macaca mulatta; D008969:Molecular Sequence Data; D011533:Proviruses / Q000235:genetics* / Q000302:isolation & purification; D016097:Simian Acquired Immunodeficiency Syndrome / Q000097:blood / Q000821:virology*; D015302:Simian Immunodeficiency Virus / Q000235:genetics*; D016662:Virus Integration
chemical_list: D004279:DNA, Viral