Analysis of protein adsorption on regenerated cellulose-based immobilized copper ion affinity membranes.
Immobilized metal affinity membranes (IMAMs) were prepared by immobilizing copper ions on microporous regenerated cellulose membranes through different types of chelating agents (dentate and triazine dye). The resulting chelator utilization percentages were 95% for iminodiacetic acid, 56% for N,N,N-tris(carboxymethyl)ethylenediamine, 52% for Cibacron blue 3GA, and 140% for Cibacron red 3BA. On the other hand, triazine dyes were slightly superior to dentate chelators on metal ion utilization for protein adsorption. In batch single-protein adsorptions, the protein adsorption capacity decreased with increasing molecular size and number of accessible surface histidine residues [lysozyme>bovine serum albumin(BSA)>gamma-globulin], while the binding strength order was the opposite (gamma-globulin>BSA>lysozyme). Moreover, the proportions of specific and nonspecific bindings were evaluated by varying pH and salt concentration conditions. A large fraction of the adsorption capacity was found to come from the nonspecific interactions for the prepared IMAMs. Lastly, batch three-protein adsorptions were performed and weak adsorption competition was observed.
Wu
Chun-Yi
CY
Department of Chemical Engineering, National Chung Hsing University, Taichung 402, Taiwan.
Suen
Shing-Yi
SY
Chen
Shiow-Ching
SC
Tzeng
Jau-Hwan
JH
D000327:Adsorption; D002482:Cellulose; D002614:Chelating Agents; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002846:Chromatography, Affinity / Q000295:instrumentation*; D004396:Coloring Agents; D003300:Copper; D006639:Histidine / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008567:Membranes, Artificial; D009113:Muramidase / Q000737:chemistry; D009994:Osmolar Concentration; D011506:Proteins / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D014227:Triazines; D005719:gamma-Globulins / Q000737:chemistry
D002614:Chelating Agents; D004396:Coloring Agents; D008567:Membranes, Artificial; D011506:Proteins; D014227:Triazines; D005719:gamma-Globulins; D012710:Serum Albumin, Bovine; D006639:Histidine; D003300:Copper; D002482:Cellulose; D009113:Muramidase