title: Classified fingerprinting: A method of comprehensive analysis for comparing megabase genomes.
abstract: The objective of the work we describe is to establish elementary methods for investigating the functions of genes; specifically, a fingerprinting method for analyzing entire DNA fragments in a mixture. Our goal is to develop a method for comparing genes with a size of several megabases. We improved a method of amplified fragment length polymorphism (AFLP) so that it could be used to analyze all the restriction fragments in a mixture. This method could be used to detect 90% of the DNA fragments produced from 100-kb model genomes by using a four-base cutter enzyme.
authors.affiliation: Biosystems Research Department, Central Research Laboratory, Hitachi Ltd.
authors.forename: K
authors.initials: K
authors.lastname: Okano
authors.forename: C
authors.initials: C
authors.lastname: Uematsu
authors.forename: M
authors.initials: M
authors.lastname: Shen
authors.forename: H
authors.initials: H
authors.lastname: Kambara
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000145:classification; D016172:DNA Fingerprinting / Q000379:methods*; D015894:Genome, Human; D023281:Genomics / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D004247:DNA