Evaluation of use of recombinant Em18 and affinity-purified Em18 for serological differentiation of alveolar echinococcosis from cystic echinococcosis and other parasitic infections.
To further evaluate recombinant Em18 antigen (rEm18) for immunodiagnosis of human alveolar echinococcosis, 208 serum samples were examined by enzyme-linked immunosorbent assay (ELISA). To comparatively assess the results of rEm18-ELISA, ELISA and immunoblot analysis with two affinity-purified native antigens were also performed with 45 selected serum samples. The results indicate that rEm18 is highly useful for serodiagnosis.
Xiao
Ning
N
Department of Parasitology, Asahikawa Medical College, Asahikawa, Japan and Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Mamuti
Wulamu
W
Yamasaki
Hiroshi
H
Sako
Yasuhito
Y
Nakao
Minoru
M
Nakaya
Kazuhiro
K
Gottstein
Bruno
B
Schantz
Peter M
PM
Lightowlers
Marshall W
MW
Craig
Philip S
PS
Ito
Akira
A
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology*; D002846:Chromatography, Affinity; D004443:Echinococcosis / Q000175:diagnosis / Q000469:parasitology*; D004444:Echinococcosis, Hepatic / Q000175:diagnosis / Q000469:parasitology; D004446:Echinococcus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting; D011994:Recombinant Proteins / Q000276:immunology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; C117889:Em18 antigen; D011994:Recombinant Proteins