title: Expression, purification, crystallization and preliminary X-ray crystallographic studies of a psychrophilic cellulase from Pseudoalteromonas haloplanktis.
abstract: The Antarctic psychrophile Pseudoalteromonas haloplanktis produces a cold-active cellulase. To date, a three-dimensional structure of a psychrophilic cellulase has been lacking. Crystallographic studies of this cold-adapted enzyme have therefore been initiated in order to contribute to the understanding of the molecular basis of the cold adaptation and the high catalytic efficiency of the enzyme at low and moderate temperatures. The catalytic core domain of the psychrophilic cellulase CelG from P. haloplanktis has been expressed, purified and crystallized and a complete diffraction data set to 1.8 A has been collected. The space group was found to be P2(1)2(1)2(1), with unit-cell parameters a = 135.1, b = 78.4, c = 44.1 A. A molecular-replacement solution, using the structure of the mesophilic counterpart Cel5A from Erwinia chrysanthemi as a search model, has been found.
authors.affiliation: Laboratoire de BioCristallographie, Institut de Biologie et Chimie des Protéines, CNRS et Université Claude Bernard Lyon 1, UMR 5086, F-69367 Lyon CEDEX 07, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Violot
authors.forename: Richard
authors.initials: R
authors.lastname: Haser
authors.forename: Guillaume
authors.initials: G
authors.lastname: Sonan
authors.forename: Daphné
authors.initials: D
authors.lastname: Georlette
authors.forename: Georges
authors.initials: G
authors.lastname: Feller
authors.forename: Nushin
authors.initials: N
authors.lastname: Aghajari
mesh_terms: D000222:Adaptation, Physiological; D020134:Catalytic Domain; D002480:Cellulase / Q000737:chemistry*; D003001:Cloning, Molecular; D003080:Cold Temperature; D003460:Crystallization; D018360:Crystallography, X-Ray; D031161:Pseudoalteromonas / Q000201:enzymology*
chemical_list: D002480:Cellulase