Quinolinate dehydrogenase and 6-hydroxyquinolinate decarboxylase involved in the conversion of quinolinic acid to 6-hydroxypicolinic acid by Alcaligenes sp. strain UK21.
In the conversion of quinolinic acid to 6-hydroxypicolinic acid by whole cells of Alcaligenes sp. strain UK21, the enzyme reactions involved in the hydroxylation and decarboxylation of quinolinic acid were examined. Quinolinate dehydrogenase, which catalyzes the first step, the hydroxylation of quinolinic acid, was solubilized from a membrane fraction, partially purified, and characterized. The enzyme catalyzed the incorporation of oxygen atoms of H(2)O into the hydroxyl group. The dehydrogenase hydroxylated quinolinic acid and pyrazine-2,3-dicarboxylic acid to form 6-hydroxyquinolinic acid and 5-hydroxypyrazine-2,3-dicarboxylic acid, respectively. Phenazine methosulfate was the preferred electron acceptor for quinolinate dehydrogenase. 6-Hydroxyquinolinate decarboxylase, catalyzing the nonoxidative decarboxylation of 6-hydroxyquinolinic acid, was purified to homogeneity and characterized. The purified enzyme had a molecular mass of approximately 221 kDa and consisted of six identical subunits. The decarboxylase specifically catalyzed the decarboxylation of 6-hydroxyquinolinic acid to 6-hydroxypicolinic acid, without any co-factors. The N-terminal amino acid sequence was homologous with those of bacterial 4,5-dihydroxyphthalate decarboxylases.
Uchida
Akira
A
Department of Biomolecular Science, Gifu University, Yanagido 1-1, 501-1193, Gifu, Japan. tonagasa@biomol.gifu-u.ac.jp
Ogawa
Mina
M
Yoshida
Toyokazu
T
Nagasawa
Toru
T
D000421:Alcaligenes / Q000201:enzymology*; D002262:Carboxy-Lyases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006900:Hydroxylation; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010088:Oxidoreductases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D010848:Picolinic Acids / Q000378:metabolism; D017378:Quinolinic Acid / Q000378:metabolism*; D011805:Quinolinic Acids; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein
C477454:6-hydroxypicolinic acid; C477453:6-hydroxyquinolinic acid; D010848:Picolinic Acids; D011805:Quinolinic Acids; D010088:Oxidoreductases; D002262:Carboxy-Lyases; D017378:Quinolinic Acid