title: Activation of soluble guanylate cyclase by NO donors--S-nitrosothiols, and dinitrosyl-iron complexes with thiol-containing ligands.
abstract: We studied the capability of dimeric forms of dinitrosyl-iron complexes and S-nitrosothiols to activate soluble guanylate cyclase (sGC) from human platelet cytosol. The dinitrosyl-iron complexes had the ligands glutathione (DNIC-GS) or N-acetylcysteine (DNIC-NAC). The S-nitrosothiols were S-nitrosoglutathione (GS-NO) or S-nitrosoacetylcysteine (SNAC). For both glutathione and N-acetylcysteine, the DNIC and S-nitrosothiol forms are equally effective activators of sGC. The activation mechanism is strongly affected by the presence of intrinsic metal ions. Pretreatment with the potent iron chelator, disodium salt of bathophenanthroline disulfonic acid (BPDS), suppressed sGC activation by GS-NO: the concentration of GS-NO producing maximal sGC activation was increased by two orders of magnitude. In contrast, activation by DNIC-GS is strongly enhanced by BPDS. When BPDS was added 10 min after supplementation of DNIC-GS or GS-NO at 4 degrees C, it exerted a similar effect on sGC activation by either NO donor: BPDS only enhanced the sGC stimulation at low concentrations of the NO donors. Our experiments demonstrated that both Fe(2+) and Cu(2+) ions contribute to the decomposition of GS-NO in the presence of ascorbate. The decomposition of GS-NO induced by Fe(2+) ions was accompanied by formation of DNIC. BPDS protected GS-NO against the destructive action of Fe(2+) but not Cu(2+) ions. Additionally, BPDS is a sufficiently strong chelator to remove the iron from DNIC-GS complexes. Based on our data, we propose that S-nitrosothiols activate sGC via a two-step iron-mediated process: In the first step, intrinsic Fe(2+) ions catalyze the formation of DNICs from S-nitrosothiols. In the secondary step, these newly formed DNICs act as the real NO donors responsible for sGC activation.
authors.affiliation: Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, 119832, Pogodinskaya Str. 10, Moscow, Russian Federation.
authors.forename: Irina S
authors.initials: IS
authors.lastname: Severina
authors.forename: Olga G
authors.initials: OG
authors.lastname: Bussygina
authors.forename: Natalya V
authors.initials: NV
authors.lastname: Pyatakova
authors.forename: Irina V
authors.initials: IV
authors.lastname: Malenkova
authors.forename: Anatoly F
authors.initials: AF
authors.lastname: Vanin
mesh_terms: D000111:Acetylcysteine / Q000378:metabolism; D001792:Blood Platelets / Q000201:enzymology / Q000378:metabolism; D003300:Copper; D004789:Enzyme Activation; D005978:Glutathione / Q000378:metabolism; D006162:Guanylate Cyclase / Q000378:metabolism*; D006801:Humans; D007501:Iron / Q000378:metabolism; D008024:Ligands; D020030:Nitric Oxide Donors / Q000378:metabolism*; D009589:Nitrogen Oxides / Q000378:metabolism; D026403:S-Nitrosothiols / Q000378:metabolism; D012995:Solubility; D013438:Sulfhydryl Compounds
chemical_list: D008024:Ligands; D020030:Nitric Oxide Donors; D009589:Nitrogen Oxides; D026403:S-Nitrosothiols; D013438:Sulfhydryl Compounds; C073661:dinitrosyl iron complex; D003300:Copper; D007501:Iron; D006162:Guanylate Cyclase; D005978:Glutathione; D000111:Acetylcysteine