title: Evaluation of use of recombinant Em18 and affinity-purified Em18 for serological differentiation of alveolar echinococcosis from cystic echinococcosis and other parasitic infections.
abstract: To further evaluate recombinant Em18 antigen (rEm18) for immunodiagnosis of human alveolar echinococcosis, 208 serum samples were examined by enzyme-linked immunosorbent assay (ELISA). To comparatively assess the results of rEm18-ELISA, ELISA and immunoblot analysis with two affinity-purified native antigens were also performed with 45 selected serum samples. The results indicate that rEm18 is highly useful for serodiagnosis.
authors.affiliation: Department of Parasitology, Asahikawa Medical College, Asahikawa, Japan and Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: Ning
authors.initials: N
authors.lastname: Xiao
authors.forename: Wulamu
authors.initials: W
authors.lastname: Mamuti
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamasaki
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Sako
authors.forename: Minoru
authors.initials: M
authors.lastname: Nakao
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Nakaya
authors.forename: Bruno
authors.initials: B
authors.lastname: Gottstein
authors.forename: Peter M
authors.initials: PM
authors.lastname: Schantz
authors.forename: Marshall W
authors.initials: MW
authors.lastname: Lightowlers
authors.forename: Philip S
authors.initials: PS
authors.lastname: Craig
authors.forename: Akira
authors.initials: A
authors.lastname: Ito
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology*; D002846:Chromatography, Affinity; D004443:Echinococcosis / Q000175:diagnosis / Q000469:parasitology*; D004444:Echinococcosis, Hepatic / Q000175:diagnosis / Q000469:parasitology; D004446:Echinococcus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting; D011994:Recombinant Proteins / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; C117889:Em18 antigen; D011994:Recombinant Proteins