Development of ribozyme synthesis system using a rolling-synchronization: effect of template DNA secondary structure on recognition of RNA polymerase.
Circular DNA behaves as an efficient template for RNA polymerase (RNAP) in rolling circle transcription (RCT). We found that the short hairpin-stem structure in the circular DNA without promoter enhances the DNA recognition of T7 RNAP in RCT. The result indicates that T7 RNAP would distinguish the short DNA hairpin-stem structure.
Ohmichi
T
T
High Technology Research Center, Faculty of Science and Engineering, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan.
Nakamura
M
M
Sugimoto
N
N
D001483:Base Sequence; D004270:DNA, Circular / Q000737:chemistry*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016337:RNA, Catalytic / Q000096:biosynthesis* / Q000737:chemistry; D013698:Templates, Genetic; D014158:Transcription, Genetic
D004270:DNA, Circular; D016337:RNA, Catalytic; D012321:DNA-Directed RNA Polymerases