Regeneration of Acer caudatifolium Hayata plantlets from juvenile explants.
Juvenile and fully mature Acer caudatifolium Hayata explants were assayed for their organogenic capacity. A protocol for multiple shoot culture formation and in vitro plant regeneration was developed for juvenile axillary bud cultures. Mature explants failed in shoot regeneration. Shoot multiplication was achieved by releasing apical dominance of the single elongated shoot on woody plant medium (WPM) supplemented with 0.7 mg l(-1) 6-benzylaminopurine and 0.05 mg l(-1) alpha-naphthaleneacetic acid. The highest rooting percentage was recorded on half-strength WPM containing 1.0 mg l(-1) indole-3-butyric acid. Regenerated plantlets were successfully hardened to ex vitro conditions and continued to grow after transfer to soil. No morphological aberrations were observed in the regenerates.
Durkovic
J
J
Department of Phytology, Technical University, Masarykova 24, 960 53, Zvolen, Slovakia. durkovic@vsld.tuzvo.sk
D031002:Acer / Q000187:drug effects / Q000254:growth & development*; D046508:Culture Techniques; D004305:Dose-Response Relationship, Drug; D007211:Indoles / Q000494:pharmacology; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development; D012038:Regeneration / Q000187:drug effects
D007211:Indoles; D009280:Naphthaleneacetic Acids; C014612:indolebutyric acid; C034182:1-naphthaleneacetic acid