title: Epidemiology of tuberculosis on Gran Canaria: a 4 year population study using traditional and molecular approaches.
abstract: BACKGROUND
  In recent years several population based studies using restriction fragment length polymorphism (RFLP) analysis have shown a higher rate of recent transmission of tuberculosis than previously thought. This study was undertaken to determine the transmission patterns of tuberculosis and the potential causes of recent transmission on the island of Gran Canaria (Spain).
  
  
  METHODS
  The strains of all patients diagnosed with tuberculosis confirmed by culture between 1 January 1993 and 31 December 1996 were typed by RFLP using the insertion sequence IS6110. A cluster was defined as two or more isolates with an identical RFLP pattern. Epidemiological linkage through contact tracing was investigated.
  
  
  RESULTS
  Of the total of 719 patients, 153 (21.3%) were excluded because there was inadequate bacterial DNA for genotyping (n=129) or the isolates of Mycobacterium tuberculosis had less than five copies of IS6110 (n=24). The isolates from 409 patients (72.3%) were grouped into 78 different clusters with an estimated 58.5% of the cases being due to recent transmission. Young age was the only significant predictor of clustering. Only in 147 (35.9%) of the 409 patients belonging to a cluster could an epidemiological link be found. 111 patients (19.6%) were identified as having had previous contact with a tuberculosis patient and 81 of them (72.9%) belonged to a cluster. The three largest clusters included 75, 49 and 20 patients, respectively.
  
  
  CONCLUSIONS
  Recent transmission is frequent among patients with tuberculosis on Gran Canaria and could be associated with certain aspects of control measures. Some of the clusters described in the study could be due to the prevalence of particular strains of M tuberculosis on the island.
authors.affiliation: Service of Microbiology, University General Hospital Dr Negrín, Las Palmas de Gran Canaria, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: Pena
authors.forename: J A
authors.initials: JA
authors.lastname: Caminero
authors.forename: M I
authors.initials: MI
authors.lastname: Campos-Herrero
authors.forename: J C
authors.initials: JC
authors.lastname: Rodríguez-Gallego
authors.forename: M I
authors.initials: MI
authors.lastname: García-Laorden
authors.forename: P
authors.initials: P
authors.lastname: Cabrera
authors.forename: M J
authors.initials: MJ
authors.lastname: Torres
authors.forename: B
authors.initials: B
authors.lastname: Lafarga
authors.forename: F
authors.initials: F
authors.lastname: Rodríguez de Castro
authors.forename: S
authors.initials: S
authors.lastname: Samper
authors.forename: F
authors.initials: F
authors.lastname: Cañas
authors.forename: D A
authors.initials: DA
authors.lastname: Enarson
authors.forename: C
authors.initials: C
authors.lastname: Martín
mesh_terms: D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D005260:Female; D006801:Humans; D015994:Incidence; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification; D012150:Polymorphism, Restriction Fragment Length; D015995:Prevalence; D012307:Risk Factors; D013030:Spain / Q000453:epidemiology; D014376:Tuberculosis / Q000453:epidemiology / Q000382:microbiology / Q000635:transmission; D014397:Tuberculosis, Pulmonary / Q000453:epidemiology* / Q000382:microbiology / Q000635:transmission