title: Purification, crystallization and preliminary X-ray analysis of a Sco1-like protein from Bacillus subtilis, a copper-binding protein involved in the assembly of cytochrome c oxidase.
abstract: The putative copper-delivery protein BsSco from Bacillus subtilis is a member of the Sco family of cytochrome c oxidase assembly proteins. BsSco is a membrane protein and the soluble domain has been cloned and expressed in Escherichia coli as a fusion with glutathione-S-transferase. The fusion protein was isolated from the cell lysate using a glutathione-affinity column and the soluble domain of BsSco was released by treatment with thrombin. Sufficient amounts of the soluble domain have been obtained for crystallization. Crystals obtained by hanging-drop vapour diffusion diffract to a resolution of 2.3 A at a synchrotron source. The space group is P6 and the unit-cell parameters are a = 67.74, b = 67.74, c = 189.58 A.
authors.affiliation: Department of Biochemistry, Queen's University, Kingston, ON K7L 3N4, Canada.
authors.forename: Iveta
authors.initials: I
authors.lastname: Imriskova-Sosova
authors.forename: Qilu
authors.initials: Q
authors.lastname: Ye
authors.forename: Bruce C
authors.initials: BC
authors.lastname: Hill
authors.forename: Zongchao
authors.initials: Z
authors.lastname: Jia
mesh_terms: D001412:Bacillus subtilis / Q000737:chemistry*; D001426:Bacterial Proteins; D003001:Cloning, Molecular; D003300:Copper; D003460:Crystallization / Q000379:methods; D003576:Electron Transport Complex IV; D005982:Glutathione Transferase; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins; D012995:Solubility; D013917:Thrombin / Q000378:metabolism; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; C482909:Sco protein, Bacillus subtilis; D003300:Copper; D003576:Electron Transport Complex IV; D005982:Glutathione Transferase; D013917:Thrombin