[Development of the human/rat chimera model with neonatal rats].
The purpose of this study was to transplant neonatal rat with human cord blood Lin(-) cells to test the possibility of this xenograft model. The Lin(-) cells were purified from human cord blood (CB) using negative selection strategy based on different lineage-specific antigens. The Lin(-) cells were injected into the liver of neonatal rats using a microinjector at an average of 5 x 10(5) cells for each. Peripheral blood (PB) and spleen were collected at 2,4 and 8 weeks after injection. Flow cytometry was performed to detect human cells in the rat PB, PCR was used to detect human cells in PB as well as spleen. The results showed that a definite proportion of human cells existed in peripheral blood of chimeric rat and the human specific beta2 microglobulin gene fragments were detected in spleen genomic DNA of chimeric rat. It is concluded that human/rat chimera model can be developed with neonatal rats. Human/rat xenograft model may provide a useful and convenient method for human hematopoietic stem cell assay in vivo.
Zhang
Yi-Kun
YK
Department of Stem Cell Biology, Institute of Transfusion Medicine, Beijing, 100850 China.
Wang
Dong-Mei
DM
Yuan
Hong-Feng
HF
Li
Hai-Min
HM
Bai
Ci-Xian
CX
Zhang
Rui
R
Chen
Lin
L
Tang
Suo-Qin
SQ
Pei
Xue-Tao
XT
D000818:Animals; D000831:Animals, Newborn; D036101:Cord Blood Stem Cell Transplantation; D004247:DNA / Q000235:genetics; D005434:Flow Cytometry; D006801:Humans; D017493:Leukocyte Common Antigens / Q000097:blood; D016133:Polymerase Chain Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000378:metabolism; D018183:Transplantation Chimera / Q000097:blood* / Q000235:genetics / Q000276:immunology; D014183:Transplantation, Heterologous; D001613:beta 2-Microglobulin / Q000235:genetics
D001613:beta 2-Microglobulin; D004247:DNA; D017493:Leukocyte Common Antigens