[The expression and activity of caspase-8 in the process of vitamin E succinate-induced apoptosis in human gastric carcinoma SGC-7901 cells].
OBJECTIVE
To investigate the role of caspase-8 in vitamin E succinate (VES)-induced apoptosis in human gastric carcinoma cells.


METHODS
The expression and activity of caspase-8 were measured by the Western Blot method with fluorescence and DAPI staining.


RESULTS
VES treatment induced the expression and activity of caspase-8 in a dose-dependent manner; the apoptosis ratio induced by VES was significantly decreased in SGC-7901 cells treated with caspase-8 inhibitor.


CONCLUSIONS
Caspase-8 plays an important role in SGC-7901 cell apoptosis induced by VES.
Li
Yao
Y
Nutrition and Food Department, Public Health College of Harbin Medical University, Harbin 150001, China.
Wu
Kun
K
Yu
Wei-ping
WP
D017209:Apoptosis / Q000187:drug effects*; D053181:Caspase 8; D020169:Caspases / Q000378:metabolism* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D013274:Stomach Neoplasms / Q000201:enzymology / Q000473:pathology*; D024505:Tocopherols; D014407:Tumor Cells, Cultured; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000494:pharmacology*
D014810:Vitamin E; C505530:CASP8 protein, human; D053181:Caspase 8; D020169:Caspases; D024505:Tocopherols