Improving quality and harvest period of protein crystals for structure-based drug design: effects of a gel and a magnetic field on bovine adenosine deaminase crystals.
The overall crystal quality as well as the harvest period of bovine adenosine deaminase containing a zinc ion at the active centre has been compared in three different environments: crystallization as a control, crystallization with agarose gel and crystallization in a high magnetic field. In crystallization with agarose gel, the probability of obtaining high-quality crystals was somewhat increased, but the harvest period was elongated. On the other hand, in crystallization in a magnetic field, the probability of obtaining high-quality crystals was greatly increased. Furthermore, the harvest period for crystal growth in a magnetic field was much shorter than that with agarose gel.
Kinoshita
Takayoshi
T
Exploratory Research Laboratories, Fujisawa Pharmaceutical Co Ltd, 5-2-3 Tokodai, Tsukuba, Ibaraki 300-2698, Japan. takayoshi_kinoshita@po.fujisawa.co.jp
Ataka
Mitsuo
M
Warizaya
Masaichi
M
Neya
Masahiro
M
Fujii
Takashi
T
D000243:Adenosine Deaminase / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D003460:Crystallization / Q000379:methods / Q000592:standards*; D018360:Crystallography, X-Ray; D015195:Drug Design; D005782:Gels; D008280:Magnetics; D011506:Proteins / Q000737:chemistry; D012685:Sepharose
D005782:Gels; D011506:Proteins; D012685:Sepharose; D000243:Adenosine Deaminase