title: Immunoglobulin G enzyme-linked immunosorbent assay using truncated nucleoproteins of Reston Ebola virus.
abstract: We developed an immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA), using partial recombinant nucleoproteins (rNP) of Reston Ebola virus (EBO-R) and Zaire Ebola virus (EBO-Z). We examined the reaction of 10 sera from cynomolgus macaques naturally infected with EBO-R to each of the partial rNP in the IgG ELISA. All the sera reacted to the C-terminal halves of the rNP of both EBO-R and EBO-Z. Most of the sera reacted to the RdeltaC (amino acid (aa) 360-739), and Rdelta6 (aa 451-551) and/or Rdelta8 (aa 631-739) at a higher dilution than to the corresponding truncated rNPs of EBO-Z. The results indicate that this IgG ELISA is useful for detecting EBO-R specific antibody, and may have a potential to discriminate EBO-R infection from other subtypes.
authors.affiliation: Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ikegami
authors.forename: M
authors.initials: M
authors.lastname: Saijo
authors.forename: M
authors.initials: M
authors.lastname: Niikura
authors.forename: M E
authors.initials: ME
authors.lastname: Miranda
authors.forename: A B
authors.initials: AB
authors.lastname: Calaor
authors.forename: M
authors.initials: M
authors.lastname: Hernandez
authors.forename: D L
authors.initials: DL
authors.lastname: Manalo
authors.forename: I
authors.initials: I
authors.lastname: Kurane
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshikawa
authors.forename: S
authors.initials: S
authors.lastname: Morikawa
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D029043:Ebolavirus / Q000276:immunology / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D019142:Hemorrhagic Fever, Ebola / Q000175:diagnosis / Q000662:veterinary*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D008252:Macaca fascicularis; D008992:Monkey Diseases / Q000175:diagnosis*; D009698:Nucleoproteins / Q000276:immunology; D012680:Sensitivity and Specificity
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D009698:Nucleoproteins