title: Cellular viability and ultrastructure of stented, antibiotic sterilized and cryopreserved sheep biological valves implanted for one year in tricuspid position.
abstract: The aim of the study was an evaluation of viability and damage of biological cells sterilized with antibiotics and cryopreserved a year after the implantation. Sheep antibiotic sterilised and cryopreserved biological valves were implanted in tricuspid position in young sheeps for one-year period. After this time the valves removed and studied morphologically. The control group consisted of 7 intact valves, the comparative group, so called group of valves after the processing antibiotic sterilization and cryopreservation consisted of 7 valves after mentioned procedures. Histologic investigations were based on paraffin sections of formalin-fixed valve cusps, stained with H&E and Masson trichrome. Valve viability was assessed using intravital staining with fluoresceine diacetate, whereas damaged cells were visualized by intravital staining with neutral red. Additionally, the ultrastructural studies were performed. The viability and ultrastructural results were compared with the pathologic process in the valve.
  
  
  CONCLUSIONS
  1. preliminary preparation with antibiotic sterilisation and cryopresrevation induces valve leaflet oedema and degenerative ultrastructural processes, colliquative cell necrosis and apoptotic morphology of a part of valve cells; 2. cryopreserved and antibiotic sterilised pulmonary valve after one-year implantation behaves lining cells, but ultrastructural changes indicates many degenerative phenomena in smaller degree than after preparation, sterilization and cryopreservation. Histopathologically the degenerative changes were prevalent.
authors.affiliation: Silesian Centre for Heart Diseases, Zabrze, Poland. jerno@poczta.wp.pl
authors.forename: Jerzy K
authors.initials: JK
authors.lastname: Nozyński
authors.forename: Henryk
authors.initials: H
authors.lastname: Grzybek
authors.forename: Ewa
authors.initials: E
authors.lastname: Zembala-Nozyńska
authors.forename: Piotr
authors.initials: P
authors.lastname: Wilczek
authors.forename: Jolanta
authors.initials: J
authors.lastname: Wszołek
authors.forename: Ireneusz
authors.initials: I
authors.lastname: Haponiuk
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002470:Cell Survival; D015925:Cryopreservation; D004203:Disinfection / Q000379:methods; D008854:Microscopy, Electron; D011664:Pulmonary Valve / Q000473:pathology / Q000503:physiopathology* / Q000637:transplantation* / Q000648:ultrastructure; D012756:Sheep; D015607:Stents; D016042:Transplantation, Heterotopic
chemical_list: D000900:Anti-Bacterial Agents