title: Decrease in expression of alpha 5 beta 1 integrin during neuronal differentiation of cortical progenitor cells.
abstract: Neuronal differentiation of embryonic neural progenitor cells is regulated by both intrinsic and extrinsic signals. Since dynamic changes in cell shape typify neuronal differentiation, cell adhesion molecules could be relevant to this process. Although it has been reported that fibronectin-integrin interactions are important for the proliferation of neural progenitor cells, little is known about the contribution of integrins to neuronal differentiation. In order to address this shortfall, we examined integrin expression on cortical progenitor cells by using immunohistochemistry and FACS analysis of cells in which GFP expression was driven by regulatory (promoter) regions of the nestin gene (nestin-GFP(+)). We here report that high levels of nestin promoter activity correlated with high expression levels of alpha(5)beta(1) integrin (alpha(5)beta(1)(high) cells). FACS analysis of nestin-GFP(+) cortical cells revealed an additional subpopulation with reduced expression of alpha(5)beta(1) integrin (alpha(5)beta(1)(low) cells). The size of the alpha(5)beta(1)(low) subpopulation increased during cortical development. To investigate the correlation between integrin and neuronal differentiation, nestin-GFP(+) cortical progenitor cells were sorted into alpha(5)beta(1)(high) or alpha(5)beta(1)(low) populations, and each potential to differentiate was analyzed. We show that the nestin-GFP(+) alpha(5)beta(1)(high) population corresponded to broadly multipotential neural progenitor cells, whereas nestin-GFP(+) alpha(5)beta(1)(low) cells appeared to be committed to a neuronal fate. These findings suggest that alpha(5)beta(1) expression on cortical progenitor cells is developmentally regulated and its downregulation is involved in the process of neuronal differentiation.
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Yoshida
authors.forename: Sohei
authors.initials: S
authors.lastname: Hishiyama
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yamaguchi
authors.forename: Masaaki
authors.initials: M
authors.lastname: Hashiguchi
authors.forename: Yusei
authors.initials: Y
authors.lastname: Miyamoto
authors.forename: Shuichi
authors.initials: S
authors.lastname: Kaminogawa
authors.forename: Tatsuhiro
authors.initials: T
authors.lastname: Hisatsune
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D019070:Cell Lineage; D002540:Cerebral Cortex / Q000166:cytology* / Q000196:embryology; D018920:Coculture Techniques; D018507:Gene Expression Regulation, Developmental; D005819:Genetic Markers; D039081:Integrin alpha5beta1 / Q000235:genetics / Q000378:metabolism*; D007381:Intermediate Filament Proteins / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins; D064231:Nestin; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D051381:Rats; D011922:Rats, Mutant Strains; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D005819:Genetic Markers; D039081:Integrin alpha5beta1; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; C577486:Nes protein, rat; D064231:Nestin