title: Molecular epidemiology of extended-spectrum beta-lactamases produced by clinical isolates in a university hospital in Greece: detection of SHV-5 in Pseudomonas aeruginosa and prevalence of SHV-12.
abstract: To assess the nature and diversity of various types of SHV and TEM derivatives in our hospital a survey was conducted. Sixty-seven extended-spectrum beta-lactamases (ESBL)-producing nosocomial pathogens, isolated over a 12-month period, were analyzed by means of PCR and direct sequencing. SHV-5 was the predominant ESBL found in our region (38 strains). Other less frequent variants included SHV-2 and SHV-12 with two and three isolates, respectively. For the first time, an outbreak of 11 Pseudomonas aeruginosa producing SHV-5 was encountered. All blaTEM-positive strains carried the non-ESBL TEM-1. The incidence of non-SHV non-TEM ESBLs was remarkably high as almost one out of three isolates harbored such an ESBL. The epidemiological and clinical impact of these findings must be carefully investigated and interpreted.
authors.affiliation: Department of Clinical Bacteriology, Parasitology, Zoonoses and Geographical Medicine, Faculty of Medicine, University of Crete, Heraklion, Greece.
authors.forename: Ioannis K
authors.initials: IK
authors.lastname: Neonakis
authors.forename: Efstathia V
authors.initials: EV
authors.lastname: Scoulica
authors.forename: Sevasti K
authors.initials: SK
authors.lastname: Dimitriou
authors.forename: Achilleas I
authors.initials: AI
authors.lastname: Gikas
authors.forename: Yannis J
authors.initials: YJ
authors.lastname: Tselentis
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005798:Genes, Bacterial / Q000235:genetics; D006115:Greece / Q000453:epidemiology; D006785:Hospitals, University; D006801:Humans; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D011552:Pseudomonas Infections / Q000453:epidemiology* / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000201:enzymology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D021521:Ribotyping; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; C107198:beta-lactamase SHV-12; C062760:beta-lactamase SHV-2; C117193:beta-lactamase SHV-5; D001618:beta-Lactamases; C034413:beta-lactamase TEM-1