title: Existence of two O-serotypes in the fish pathogen Pseudomonas anguilliseptica.
abstract: The serological characteristics of a group of 32 Pseudomonas anguilliseptica strains isolated in Spain from seabream (Sparus aurata) and turbot (Scophthalmus maximus) were compared with a total of 18 collection strains of this bacterial species with different geographical and host origin. The employment of different techniques, including slide agglutination, microagglutination and dot blot, allowed us to establish two serological groups, one comprising practically all the eel isolates, and the other including the majority of isolates from other fish species. The study of the lipopolysaccharides (LPS) and outer membrane proteins (OMP) corroborated these results, indicating that the serological differences among strains are due to the somatic antigen and not to antigenic determinants of protein nature. Therefore, a serological scheme of two "O" serotypes is proposed for P. anguilliseptica. The results obtained will be of importance for epidemiological studies as well as for the design of adequate vaccine formulations.
authors.affiliation: Departamento de Microbiología y Parasitología, Facultad de Biología, Universidad de Santiago, 15782 Santiago de Compostela, Spain.
authors.forename: Sonia
authors.initials: S
authors.lastname: López-Romalde
authors.forename: Beatriz
authors.initials: B
authors.lastname: Magariños
authors.forename: Carmen
authors.initials: C
authors.lastname: Ravelo
authors.forename: Alicia E
authors.initials: AE
authors.lastname: Toranzo
authors.forename: Jesús L
authors.initials: JL
authors.lastname: Romalde
mesh_terms: D000372:Agglutination Tests / Q000662:veterinary; D000811:Anguilla / Q000382:microbiology; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D005393:Fish Diseases / Q000382:microbiology*; D005412:Flatfishes / Q000382:microbiology*; D015151:Immunoblotting / Q000662:veterinary; D008070:Lipopolysaccharides / Q000032:analysis; D010802:Phylogeny; D011549:Pseudomonas / Q000145:classification* / Q000302:isolation & purification; D011552:Pseudomonas Infections / Q000382:microbiology / Q000662:veterinary*; D011817:Rabbits; D021541:Sea Bream / Q000382:microbiology*; D012703:Serotyping / Q000662:veterinary; D013030:Spain
chemical_list: D001425:Bacterial Outer Membrane Proteins; D008070:Lipopolysaccharides