title: Differential hyperacetylation of histones H3 and H4 upon promoter-specific recruitment of EBNA2 in Epstein-Barr virus chromatin.
abstract: Epstein-Barr virus nuclear antigen 2 (EBNA2) is a transcriptional activator involved in the immortalization of B lymphocytes by the virus. EBNA2 is targeted to the promoters of its responsive genes, via interaction with cellular DNA-binding proteins. Using chromatin immunoprecipitation assays, we show for the first time the conditional recruitment of EBNA2 on two specific viral promoters in vivo and demonstrate a correlation between this recruitment and a local change in the acetylation of histones H3 and H4, which is promoter dependent.
authors.affiliation: Unité de Virologie Humaine, U412 INSERM, Ecole Normale Supérieure de Lyon, 46 Allée d'Italie, 69364 Lyon Cedex 07, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Alazard
authors.forename: Henri
authors.initials: H
authors.lastname: Gruffat
authors.forename: Edwige
authors.initials: E
authors.lastname: Hiriart
authors.forename: Alain
authors.initials: A
authors.lastname: Sergeant
authors.forename: Evelyne
authors.initials: E
authors.lastname: Manet
mesh_terms: D000107:Acetylation; D002460:Cell Line; D002472:Cell Transformation, Viral; D002843:Chromatin / Q000378:metabolism*; D019309:Epstein-Barr Virus Nuclear Antigens / Q000235:genetics / Q000378:metabolism*; D015967:Gene Expression Regulation, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000502:physiology*; D006657:Histones / Q000378:metabolism*; D006801:Humans; D011233:Precipitin Tests; D011401:Promoter Regions, Genetic; D014764:Viral Proteins
chemical_list: D002843:Chromatin; C402641:EBNA-2 protein, Human herpesvirus 4; D019309:Epstein-Barr Virus Nuclear Antigens; D006657:Histones; D014764:Viral Proteins