title: Manganese, copper, and zinc in cerebrospinal fluid from patients with multiple sclerosis.
abstract: The concentrations of manganese, copper, and zinc in cerebrospinal fluid (CSF) from patients with multiple sclerosis (MS) and patients with no known neurological disease (control group) were measured. Manganese and copper levels were determined by two different analytical methods: atomic absorption spectrometry (AAS) and high-resolution inductively coupled plasma-mass spectrometry (HR-ICP-MS), whereas zinc levels were determined by HR-ICP-MS only. Manganese levels (mean+/-SEM) were significantly decreased in the CSF of MS patients (1.07+/-0.13 microg/L, ICP-MS; 1.08+/-0.11 microg/L, AAS) compared to the levels in the control group (1.78+/-0.26 microg/L, ICP-MS; 1.51+/-0.17 microg/L, AAS). Copper levels were significantly elevated in the CSF of MS patients (10.90+/-1.11 microg/L; ICP-MS, 11.53+/-0.83 microg/L, AAS) compared to the levels in the control group (8.67+/-0.49 microg/L, ICP-MS; 9.10+/-0.62 microg/L, AAS). There were no significant differences between the CSF zinc levels of MS and control patients. The physiological basis for the differences in manganese and copper concentrations between MS patients and controls is unknown, but could be related to alterations in the manganese- containing enzyme glutamine synthetase and the copper-containing enzyme cytochrome oxidase.
authors.affiliation: Department of Chemistry, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.
authors.forename: Torun M
authors.initials: TM
authors.lastname: Melø
authors.forename: Cecilie
authors.initials: C
authors.lastname: Larsen
authors.forename: Linda R
authors.initials: LR
authors.lastname: White
authors.forename: Jan
authors.initials: J
authors.lastname: Aasly
authors.forename: Torill E
authors.initials: TE
authors.lastname: Sjøbakk
authors.forename: Trond P
authors.initials: TP
authors.lastname: Flaten
authors.forename: Ursula
authors.initials: U
authors.lastname: Sonnewald
authors.forename: Tore
authors.initials: T
authors.lastname: Syversen
mesh_terms: D003300:Copper / Q000134:cerebrospinal fluid*; D005260:Female; D006801:Humans; D008297:Male; D008345:Manganese / Q000134:cerebrospinal fluid*; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid*; D013054:Spectrophotometry, Atomic; D015032:Zinc / Q000134:cerebrospinal fluid*
chemical_list: D008345:Manganese; D003300:Copper; D015032:Zinc