Cross-species amplification from crop soybean Glycine max provides informative microsatellite markers for the study of inbreeding wild relatives.
The development of microsatellite markers through transfer of primers from related species (cross-species amplification) remains a little-explored alternative to the de novo method in plants. In this study of 100 microsatellite loci from Glycine max, we examined two aspects of primer transfer. First, we tested if source locus properties can predict primer transfer and polymorphism in Glycine cyrtoloba and Glycine clandestina. We transferred 23 primers to G. cyrtoloba and 42 to G. clandestina, with 19 loci polymorphic within G. clandestina. However, we could not predict transfer or polymorphism from the source locus properties. Second, we evaluated the subset of 11 polymorphic loci for study in G. clandestina populations representing two local morphotypes. All loci were informative within populations (population mean He +/- SE = 0.58 +/- 0.04). We directly sequenced 28 alleles at 4 representative loci. The allelic patterns and sequencing results established that 8 of 11 loci were typical microsatellites, confirming the utility of primer transfer as an alternative to de novo development. Additionally, we found that morphotypic differentiation between populations was paralleled by changes in polymorphism level at six loci and size homoplasy at one locus. We interpret these patterns as being a product of selfing in G. clandestina. Our results demonstrate the value of allele sequence knowledge for the most effective use of microsatellites.
Hempel
K
K
School of Botany and Zoology, Building 116, Australian National University, Acton, ACT 0200, Australia. kaska.hempel@anu.edu.au
Peakall
R
R
D001315:Australia; D001483:Base Sequence; D005787:Gene Frequency; D014644:Genetic Variation; D007178:Inbreeding; D018895:Microsatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D012098:Reproduction / Q000502:physiology; D017422:Sequence Analysis, DNA; D013025:Glycine max / Q000235:genetics*