Dependence of exogenous SERCA gene expression on coxsackie adenovirus receptor levels in neonatal and adult cardiac myocytes.
We demonstrate that the efficiency of adenovirus-assisted exogenous Ca(2+) ATPase (SERCA) and reporter (EGFP) gene expression is much higher in primary cultures of myocytes from neonatal rat hearts, than in primary cultures of myocytes from adult rat hearts. In this respect, the neonatal myocytes behave similarly to the established COS-1 cell line. This difference is related to the level of coxsackie adenovirus receptor (CAR) that affects cell penetration and expression level of exogenous genes, and explains variations in the observed consequences of exposure to adenovirus vector carrying SERCA cDNA. Awareness of these differences should be highly advantageous in complementary studies of exogenous gene expression in neonatal and adult myocytes. It should also be advantageous in evaluating conditions yielding optimal ratios of functional benefits over possible toxic effects upon exogenous SERCA gene delivery to cardiac muscle.
Sumbilla
Carlota
C
Department of Biochemistry, University of Maryland School of Medicine, 108 N Greene Street, Baltimore, MD 21201-1503, USA.
Ma
Hailun
H
Seth
Malini
M
Inesi
Giuseppe
G
D000328:Adult; D000375:Aging / Q000502:physiology; D000818:Animals; D000831:Animals, Newborn; D019556:COS Cells; D000252:Calcium-Transporting ATPases / Q000096:biosynthesis* / Q000235:genetics*; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D004770:Enterovirus / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D006801:Humans; D032383:Myocytes, Cardiac / Q000166:cytology / Q000201:enzymology* / Q000502:physiology / Q000821:virology; D051381:Rats; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism*; D012016:Reference Values; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases
C568771:CLMP protein, human; C568773:Clmp protein, rat; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D011991:Receptors, Virus; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D000252:Calcium-Transporting ATPases