title: Vacancy ion-exclusion chromatography of aromatic carboxylic acids on a weakly acidic cation-exchange resin.
abstract: Determination of aromatic carboxylic acids by conventional ion-exclusion chromatography is relatively difficult and methods generally rely on hydrophobic interaction between the solute and the resin. To overcome the difficulties in determining aromatic carboxylic acids a new approach is presented, termed vacancy ion-exclusion chromatography, which is based on use of the sample as mobile phase and an injection of aqueous 10% methanol onto a weakly acidic cation-exchange column (TSKgel OApak-A). Highly sensitive conductivity detection occurred with sharp and well-shaped peaks, leading to very efficient separations. The effects of sulfuric acid concentration added to the mobile phase, flow-rate, and column temperature on the retention volume of tested aromatic carboxylic acids was investigated. Retention times were found to be affected by the concentration of the analytes in the mobile phase and to some extent also by the addition of an organic modifier such as methanol to the injected water sample. Separation of sulfuric acid (SA), naphthalenetetracarboxylic acid (NTCA), phthalic acid (PA) and benzoic acid (BA) was satisfactory using this new approach. Detection limits were 0.66, 0.67, 0.42 and 0.86 microM and detector responses were linear in the range 1-100, 1-80, 2.5-100 and 10-40 microM, for SA, NTCA, PA and BA, respectively. Precision for retention times was 0.36% and for peak areas was 1.5%.
authors.affiliation: National Institute of Advanced Industrial Science and Technology, Graduate School of Engineering, Chubu University, Seto 489-0884, Japan. murad.helaleh@lycos.com
authors.forename: Murad I H
authors.initials: MI
authors.lastname: Helaleh
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Tanaka
authors.forename: Masanobu
authors.initials: M
authors.lastname: Mori
authors.forename: Qun
authors.initials: Q
authors.lastname: Xu
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Taoda
authors.forename: Ming-Yu
authors.initials: MY
authors.lastname: Ding
authors.forename: Wenzhi
authors.initials: W
authors.lastname: Hu
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Hasebe
authors.forename: Paul R
authors.initials: PR
authors.lastname: Haddad
mesh_terms: D019817:Benzoic Acid; D002264:Carboxylic Acids / Q000302:isolation & purification*; D002411:Cation Exchange Resins; D002852:Chromatography, Ion Exchange / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D000432:Methanol; D009281:Naphthalenes / Q000302:isolation & purification; D010795:Phthalic Acids / Q000302:isolation & purification; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013464:Sulfuric Acids / Q000302:isolation & purification; D013696:Temperature
chemical_list: D002264:Carboxylic Acids; D002411:Cation Exchange Resins; D009281:Naphthalenes; D010795:Phthalic Acids; D013464:Sulfuric Acids; C032279:phthalic acid; D019817:Benzoic Acid; C033158:sulfuric acid; D000432:Methanol