title: Fractal calibration in size-exclusion chromatography I. An introduction.
abstract: The elution behaviour of different polymer-solvent systems in three types of organic columns for SEC has been compared and interpreted. The experimental data show that the classical universal calibration is not accomplished. Deviations from a unique curve are observed due to the binary and ternary interactions between the components of the system (solvent, polymer and gel) which results on secondary mechanisms accompanying the main pure or "ideal" SEC separation mechanism. Both, enthalpic and entropic effects are interpreted in terms of the swelling and crosslinking degrees of the gel packings, and are also related with the fractal characteristics of their surfaces, such as the fractal dimension and the available pore size. Moreover, a relationship between the fractal dimension of the pore surface and the chromatographic distribution coefficient is proposed.
authors.affiliation: Department de Química Física and Institut de Ciéncia dels Materials, Universitat de València, E-46100 Burjassot, València, Spain.
authors.forename: Rosa
authors.initials: R
authors.lastname: García-Lopera
authors.forename: Isabel
authors.initials: I
authors.lastname: Irurzun
authors.forename: Concepción
authors.initials: C
authors.lastname: Abad
authors.forename: Agustín
authors.initials: A
authors.lastname: Campos
mesh_terms: D002138:Calibration; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002850:Chromatography, Gel; D017709:Fractals; D005782:Gels; D011108:Polymers; D012997:Solvents; D014783:Viscosity
chemical_list: D005782:Gels; D011108:Polymers; D012997:Solvents