title: Effects of intraoperative autologous blood donation and tepid temperature cardiopulmonary bypass on blood system.
abstract: The effects of the intraoperative autologous blood donation and tepid temperature cardiopulmonary bypass (CPB) on blood system were investigated. Twenty-four patients with rheumatic heart valve diseases scheduled for open heart surgery were selected and divided randomly into group A (intraoperative autologous blood donation and tepid temperature, nasopharyngeal temperature was at 32-34 degrees C during CPB) and group B (control, nasopharyngeal temperature was at 25-28 degrees C during CPB). The plasmatic concentrations of GMP-140 and D-Dimer and the plasmatic activities of 6-keto-PGF1 alpha and AT-III were measured by using ELISA or substrate luminescence techniques before operation, at the end of CPB, after discontinuation of CPB and postoperatively. Red blood cell count, platelet count, hematocrit, the amount of blood drainage and the amount of blood transfusion needed were measured or recorded postoperatively. The results showed the plasmatic concentrations of GMP-140 and D-Dimer in group A were significantly less (P < 0.05) than those in group B during and after operation. The activity of 6-keto-PGF1 alpha in group A was higher (P < 0.05) than that in group B during and after operation. The AT-III activity in group A was less (P < 0.05) during CPB but higher 30 min after discontinuation of CPB than that in group B. The amount of postoperative blood loss (283 +/- 166 versus 722 +/- 194 ml, P < 0.01) and amount of blood transfusion (816 +/- 126 versus 1443 +/- 678 ml, P < 0.01) in group A were significantly less than those in group B, respectively. The red blood cell count, platelet count and hematocrit in group A were significantly higher than those in group B after operation. The results suggests intraoperative autologous blood donation and tepid temperature have a good protection on blood system and can reduce postoperative non-surgical bleeding.
authors.affiliation: Department of Anesthesiology, Xiehe Hospital, Tongji Medical University, Wuhan 430022.
authors.forename: S
authors.initials: S
authors.lastname: Zhang
authors.forename: S
authors.initials: S
authors.lastname: Yao
mesh_terms: D000328:Adult; D000990:Antithrombin III / Q000378:metabolism*; D001804:Blood Transfusion, Autologous; D002315:Cardiopulmonary Bypass; D005260:Female; D005341:Fibrinolysin / Q000378:metabolism; D006349:Heart Valve Diseases / Q000097:blood / Q000601:surgery; D019918:Heart Valve Prosthesis Implantation; D006801:Humans; D007036:Hypothermia, Induced; D007432:Intraoperative Period; D008297:Male; D008875:Middle Aged; D010976:Platelet Count; D012214:Rheumatic Heart Disease / Q000601:surgery; D013696:Temperature
chemical_list: D000990:Antithrombin III; D005341:Fibrinolysin