Patatin, the tuber storage protein of potato (Solanum tuberosum L.), exhibits antioxidant activity in vitro.
The potato (Solanum tuberosum L.) tuber storage protein, patatin, was purified to homogeneity with a molecular mass of 45 kDa. The purified patatin showed antioxidant or antiradical activity by a series of in vitro tests, including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical (half-inhibition concentration, IC(50), was 0.582 mg/mL) scavenging activity assays, anti-human low-density lipoprotein peroxidation tests, and protections against hydroxyl radical-mediated DNA damages and peroxynitrite-mediated dihydrorhodamine 123 oxidations. Using electron paramagnetic resonance (EPR) spectrometry for hydroxyl radical detections, it was found that the intensities of the EPR signal were decreased by the increased amounts of patatin added (IC(50) was 0.775 mg/mL). Through modifications of patatin by iodoacetamide or N-bromosuccinimide, it was found that the antiradical activities of modified patatin against DPPH or hydroxyl radicals were decreased. It was suggested that cysteine and tryptophan residues in patatin might contribute to its antioxidant activities against radicals.
Liu
Yen-Wenn
YW
School of Pharmacy, Taipei Medical University, Taipei 110, Taiwan, Republic of China.
Han
Chuan-Hsiao
CH
Lee
Mei-Hsien
MH
Hsu
Feng-Lin
FL
Hou
Wen-Chi
WC
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds; D002265:Carboxylic Ester Hydrolases / Q000302:isolation & purification / Q000494:pharmacology*; D002417:Cattle; D003300:Copper / Q000494:pharmacology; D004249:DNA Damage / Q000187:drug effects; D004578:Electron Spin Resonance Spectroscopy; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D017665:Hydroxyl Radical / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008077:Lipoproteins, LDL / Q000737:chemistry; D010851:Picrates / Q000737:chemistry; D010940:Plant Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D011198:Solanum tuberosum / Q000737:chemistry*; D013950:Thymus Gland / Q000737:chemistry
D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D008077:Lipoproteins, LDL; D010851:Picrates; D010940:Plant Proteins; C043222:patatin protein, Solanum tuberosum; D017665:Hydroxyl Radical; D003300:Copper; C004931:1,1-diphenyl-2-picrylhydrazyl; D002265:Carboxylic Ester Hydrolases