[Cloning of p53/p21 fusion gene and it's inhibitory effect on the growth in Tca8113 cells].
OBJECTIVE
To study the p53/p21 fusion gene as a potential fusion gene for the gene therapy of human oral squamous cell carcinoma.


METHODS
p21 cDNA was obtained from normal human embryonic lung cells by RT-PCR, fusing with p53 gene. The recombinant plasmid pcDNA-p53/p21 was constructed by inserting the p53/p21 fusion gene into eukaryotic expression vector pcDNA3.1 and subsequently transfected into human oral squamous cell carcinoma cell line (Tca8113) with lipofectamine. RT-PCR and Western blot were used to demonstrate the expression of p53/p21 fusion gene. Using clonal formation experiment and (3)H-TdR incorporation assay were used to evaluate the clonal formation and proliferation ability of Tca8113 cells.


RESULTS
It was observed that p53/p21 fusion gene could inhibit clonal formation and proliferation of human oral carcinoma. RT-PCR and Western blot demonstrated that it was the expression of exogenous p53/p21 fusion gene that led to the above results.


CONCLUSIONS
Transfection of p53/p21 fusion gene to Tca8113 cells could inhibit the tumor cell proliferation and clone formation in vitro, and make itself a potential fusion gene for the gene therapy of human oral squamous cell carcinoma.
Yu
You-cheng
YC
Department of Dentistry, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Gu
Zhang-yu
ZY
Chen
Wan-tao
WT
Zhang
Zhi-yuan
ZY
D002294:Carcinoma, Squamous Cell / Q000628:therapy; D045744:Cell Line, Tumor; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics*; D050939:Gene Fusion / Q000235:genetics*; D016158:Genes, p53 / Q000235:genetics*; D015316:Genetic Therapy; D006801:Humans; D009062:Mouth Neoplasms / Q000628:therapy
D050759:Cyclin-Dependent Kinase Inhibitor p21