Uptake of photofrin II, a photosensitizer used in photodynamic therapy, by tumour cells in vitro.
Photosensitizing dyes are used in fluorescence diagnostics and photodynamic therapy (PDT). These usually hematoporphyrin derivatives (HpD) accumulate preferentially within neoplastic tissues. HpD is a mixture of ether and ester linked porphyrins. Its partially purified form is known under the commercial name of photofrin II (PII). PII emission spectra were studied in a hydrophilic (PBS) and a lipophilic (PC liposomes) environment. Red shift was observed in their emission maxima from 615 nm in buffer solution to 635 nm in lipid. Identical red shift was obtained when the intracellular fluorescence of two cancer cell lines, MCF 7 and Jurkat, incubated with PII was investigated. Thus, intramembrane localization of PII may be suggested. As determined from the total fluorescence intensity, the uptake of PII was only slightly higher for Jurkat than for MCF 7 cells. Nevertheless the kinetics of the uptake was found to be different for both cell lines.
Chwiłkowska
Agnieszka
A
Department of Medical Biochemistry, Wrocław Medical University, T. Chałubińskiego 10, 50-368 Wrocław, Poland. maria@bioch.am.wroc.pl
Saczko
Jolanta
J
Modrzycka
Teresa
T
Marcinkowska
Anna
A
Malarska
Anna
A
Bielewicz
Julita
J
Patalas
Dorota
D
Banaś
Teresa
T
D000230:Adenocarcinoma / Q000378:metabolism*; D001943:Breast Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D017323:Dihematoporphyrin Ether / Q000493:pharmacokinetics*; D005260:Female; D006801:Humans; D019169:Jurkat Cells; D015458:Leukemia, T-Cell / Q000378:metabolism*; D008081:Liposomes / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry; D010778:Photochemotherapy / Q000379:methods; D017319:Photosensitizing Agents / Q000493:pharmacokinetics*; D013050:Spectrometry, Fluorescence
D008081:Liposomes; D010713:Phosphatidylcholines; D017319:Photosensitizing Agents; D017323:Dihematoporphyrin Ether