title: Role of Caenorhabditis elegans protein phosphatase type 1, CeGLC-7 beta, in metaphase to anaphase transition during embryonic development.
abstract: In Caenorhabditis elegans embryogenesis, phosphorylation events are critical to chromosomal changes. To investigate the dephosphorylation of chromosome behavior, we cloned and characterized the cDNA that encodes C. elegans protein phosphatase type 1 (CeGLC-7 beta), which is composed of 333 amino acids. CeGLC-7 beta possesses a highly conserved amino acid sequence with mammalian and Drosophila protein phosphatase 1. Here, we report on the contribution of CeGLC-7 beta to the dephosphorylation of histone H3 at anaphase. At the embryonic stage, CeGLC-7 beta is associated with the nuclear membrane and chromosomes. The deletion of the Ceglc-7 beta gene and a microinjection of double-stranded RNA produce a disorganized embryogenesis. The Ceglc-7 beta gene mutation causes an abnormal accumulation of phosphorylated histone H3 and delays the mitotic process after anaphase. We propose that CeGLC-7 beta is involved in chromosome dynamics including histone H3 dephosphorylation.
authors.affiliation: Department of Physical Information, Faculty of Medicine, Kanazawa University, 5-11-80 Kodatsuno, Kanazawa, Ishikawa 920-0942, Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Sassa
authors.forename: Hiroko
authors.initials: H
authors.lastname: Ueda-Ohba
authors.forename: Kei-ichiro
authors.initials: K
authors.lastname: Kitamura
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Harada
authors.forename: Ryuji
authors.initials: R
authors.lastname: Hosono
mesh_terms: D000595:Amino Acid Sequence; D000705:Anaphase / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000196:embryology / Q000201:enzymology* / Q000235:genetics; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D017124:Conserved Sequence; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000378:metabolism; D017353:Gene Deletion; D018507:Gene Expression Regulation, Developmental; D017238:Genes, Helminth; D049452:Green Fluorescent Proteins; D006657:Histones / Q000378:metabolism; D008164:Luminescent Proteins / Q000378:metabolism; D008677:Metaphase / Q000235:genetics; D008969:Molecular Sequence Data; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D054645:Protein Phosphatase 1; D012330:RNA, Double-Stranded / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D029742:Caenorhabditis elegans Proteins; D006657:Histones; D008164:Luminescent Proteins; D012330:RNA, Double-Stranded; D034741:RNA, Small Interfering; D049452:Green Fluorescent Proteins; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1