Synthesis of probes with broad pH range fluorescence.
Reaction of a rhodamine 2'-ester with an excess of alkyldiamines provides amino-functionalized rhodamine spirolactams, which when subsequently conjugated with carboxyfluorescein, provides probes which are fluorescent at acidic, neutral, and basic pH ranges.
Adamczyk
Maciej
M
Department of Chemistry (D9MD), Abbott Diagnostics Division, Abbott Laboratories, 100 Abbott Park Road, 60064-6016, Abbott Park, IL, USA. maciej.adamczyk@abbott.com
Grote
Jonathan
J
D002463:Cell Membrane Permeability; D002851:Chromatography, High Pressure Liquid; D005452:Fluoresceins / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007769:Lactams / Q000138:chemical synthesis / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D012235:Rhodamines / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D005452:Fluoresceins; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D007769:Lactams; D012235:Rhodamines; C024098:6-carboxyfluorescein