title: Molecular cloning of quinohemoprotein alcohol dehydrogenase, ADH IIB, from Pseudomonas putida HK5.
abstract: Molecular cloning of the gene of quinohemoprotein alcohol dehydrogenase (ADH IIB) from Pseudomonas putida HK5 was done. The gene (qbdA) was 690 amino acids in length, containing a 22-amino acid signal sequence. Another gene (qbdB) upstream of qbdA, probably in the same transcriptional unit, was found. Further upstream, a gene divergently transcribed against qbdBA had identity with NAD-dependent aldehyde dehydrogenase.
authors.affiliation: Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, Japan. toyama@agr.yamaguchi-u.ac.jp
authors.forename: Hirohide
authors.initials: H
authors.lastname: Toyama
authors.forename: Takaaki
authors.initials: T
authors.lastname: Fujii
authors.forename: Nahoko
authors.initials: N
authors.lastname: Aoki
authors.forename: Kazunobu
authors.initials: K
authors.lastname: Matsushita
authors.forename: Osao
authors.initials: O
authors.lastname: Adachi
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D002874:Chromosome Mapping; D003001:Cloning, Molecular / Q000379:methods*; D040461:Gene Components; D008969:Molecular Sequence Data; D005810:Multigene Family; D016958:Pseudomonas putida / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D000429:Alcohol Oxidoreductases; C019641:alcohol dehydrogenase (acceptor)