Protein-conjugated nanoparticles from rapid expansion of supercritical fluid solution into aqueous solution.
The method of rapid expansion of a supercritical solution into a liquid solvent (RESOLV) was applied to the preparation of bovine serum albumin protein-conjugated silver sulfide nanoparticles. The conjugate samples were characterized by using a series of instrumental techniques. The results show that the monodispersed nanoparticles in the conjugates are well-coated directly with the protein. Because the protein undergoes solution pH-dependent association and dissociation, the protein-nanoparticle conjugates also assemble and disassemble with changes in solution pH in a reversible fashion.
Meziani
Mohammed J
MJ
Department of Chemistry and Center for Advanced Engineering Fibers and Films, Howard L. Hunter Chemistry Laboratory, Clemson University, Clemson, SC 29634-0973, USA.
Sun
Ya-Ping
YP
D006863:Hydrogen-Ion Concentration; D018625:Microscopy, Atomic Force; D008854:Microscopy, Electron; D036103:Nanotechnology; D010316:Particle Size; D011487:Protein Conformation; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D018030:Silver Compounds / Q000737:chemistry*; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D014867:Water / Q000737:chemistry*
D018030:Silver Compounds; D012996:Solutions; D014867:Water; D012710:Serum Albumin, Bovine; C013251:silver sulfide