title: Photo-regulation of RNA polymerase reaction by use of modified DNA carrying an azobenzene.
abstract: Transcription reaction by T7-RNA polymerase was photo-regulated on the basis of two strategies as depicted in Scheme 1. It was found that incorporation reaction of azobenzene-tethered uridine triphosphate proceeded only when azobenzene took trans-form (Scheme 1(A)). On the other hand, the transcription was more efficient when the azobenzene moiety, tethered to the non-template strand of the promoter DNA, was in its cis-form under UV irradiation (Scheme 1(B)). Thus, azobenzene-tethered DNAs are promising for the photo-regulation of gene-expression.
authors.affiliation: Research Center for Advanced Science and Technology, Univ. of Tokyo, Komaba, Meguro-ku, Tokyo 153-8904, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Asanuma
authors.forename: D
authors.initials: D
authors.lastname: Tamaru
authors.forename: T
authors.initials: T
authors.lastname: Yoshida
authors.forename: A
authors.initials: A
authors.lastname: Yamazawa
authors.forename: T
authors.initials: T
authors.lastname: Mitsui
authors.forename: T
authors.initials: T
authors.lastname: Okuni
authors.forename: M
authors.initials: M
authors.lastname: Kimoto
authors.forename: I
authors.initials: I
authors.lastname: Hirao
authors.forename: S
authors.initials: S
authors.lastname: Yokoyama
authors.forename: M
authors.initials: M
authors.lastname: Komiyama
mesh_terms: D001391:Azo Compounds / Q000737:chemistry*; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D005786:Gene Expression Regulation; D008957:Models, Genetic; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic / Q000528:radiation effects*; D014466:Ultraviolet Rays; D014544:Uridine Triphosphate / Q000737:chemistry; D014764:Viral Proteins
chemical_list: D001391:Azo Compounds; D014764:Viral Proteins; D004247:DNA; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases; C009850:azobenzene; D014544:Uridine Triphosphate