[Experimental and clinical study on the chemosensitivity assay in vitro for head and neck tumor by AgNOR methods].
OBJECTIVE
To evaluate in vitro chemosensitivity of head and neck tumor to eight kinds of antineoplastic drugs by AgNOR technique.


METHODS
Fifty-nine cases' fresh specimens were obtained from head and neck tumors. We severed tumor tissue with simple mechanical separation method. After the acting of drugs, cell smear and argyophilie staining, mean Inhibiting rate was calculated. 12 cases were treated with chemotherapy at the same time.


RESULTS
54 cases' specimens succeeded in the experiments, the rate of success was 91.5%. The changes of mean Inhibiting rate were from 0 to 63.0%. The chemosensitivity to DDP was the highest in eight kinds of antineoplastic drugs. VCR, 5-Fu, PYM were higher sensitive drugs respectively. The sensitivity to drugs in NPC was significantly higher than laryngeal cancer. The coincidence between the experiment and clinic was 75.0%.


CONCLUSIONS
AgNOR method is simple and convenient. In addition, it has clear correlation with the results of therapy. The method is useful for chemotherapy.
Hu
Guohua
G
Department of Otolaryngology, First Affilated Hospital, Chongqing Medical University, Chongqing 400016.
Zhu
Jiang
J
Sun
Yucai
Y
Wei
Lianzhi
L
D000368:Aged; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D005260:Female; D006258:Head and Neck Neoplasms / Q000473:pathology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016622:Silver Staining; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000970:Antineoplastic Agents