[Expression and significance of proliferation marker and apoptosis-related genes in benign and malignant epithelial lesions of the larynx].
OBJECTIVE
In order to study the ki-67, bcl-2, bax proteins expression and significance in the benign and malignant epithelium of the larynx.


METHODS
An immunohistochemical S-P assay was used to detect the expression of ki-67, bcl-2, bax in LSCCs, epithelial dysplasia, papillomas, simple hyperplasia and normal laryngeal epithelium.


RESULTS
The mean value of ki-67 was 52.0%, 50.0%, 47.8%, 41.6%, 10.0% respectively. The different was statistically significant between LSCCS or dysplasia and normal laryngeal epithelial (P < 0.05). The mean value of bcl-2 was 44.0% in LSCCs, 11.0% in dysplasia, 0% in other three epithelial lesions of the larynx. Bcl-2 was highly positive in LSCCs compared to the other lesions (P < 0.05, P < 0.01, P < 0.01). The expression of bax was 76.0%, 77.8%, 69.6%, 58.3%, 80.0%. In papillomas, there was statistically significant positive correlation between Bcl-2 and ki-67.


CONCLUSIONS
Ki-67 and Bcl-2 coexpression may play a role in the uncontrolled proliferation in papillomas. Ki-67, bax, Bcl-2 could help in estimation the premalignant stages of the larynx.
Bai
Yan
Y
Department of Otolaryngology, Children's Hospital, Chongqing Medical University, Chongqing 400014.
Hong
Suling
S
Peng
Zhennian
Z
D017209:Apoptosis / Q000235:genetics; D002455:Cell Division / Q000235:genetics; D019254:Genes, bcl-2; D006801:Humans; D019394:Ki-67 Antigen / Q000096:biosynthesis*; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D010212:Papilloma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis*; D051028:bcl-2-Associated X Protein
C494826:BAX protein, human; D019394:Ki-67 Antigen; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein