Rapid onset of nucleolar disintegration preceding cell cycle arrest in roscovitine-induced apoptosis of human MCF-7 breast cancer cells.
The aim of our study was to explore the antiproliferative and pro-apoptotic action of roscovitine (ROSC) on human breast cancer MCF-7 cells. We examined the effect of ROSC on cell proliferation, cell cycle progression, nucleolar morphology, posttranslational modifications of histones as well as on induction of apoptosis. The effects of ROSC on the argyrophilic nucleolar organizer regions (AgNORs) and nucleolar RNA of MCF-7 cells were marked: ROSC treatment changed the pattern of AgNORs in a time-dependent manner. The disintegration of nucleoli manifested by increasing number of nucleolar fragments already began at 6 hr posttreatment. This was accompanied by a redistribution of the nucleolin from the nucleolus beginning after 6 hr and preceded a decrease of histone acetylation and phosphorylation. Inhibition of DNA synthesis and accumulation of G(2)/M-arrested cells starting 6 hr posttreatment coincided with a strong increase of the p53 level and with an appearance of a few cells committed to undergo apoptosis. However, all these changes preceded the main wave of apoptosis, which occurred after 24 hr ROSC treatment as assessed by determination of the frequency of Annexin binding, activation of caspases as well as of DNA fragmentation. Onset of PARP-1 cleavage detected by immunoblotting and by immunohistochemistry 6 hr or 9 hr posttreatment, respectively, preceded for a few hours the DNA fragmentation detected in situ by TUNEL assay. Reconstitution of MCF-7 cells with caspase-3 did not change the kinetics of ROSC-induced apoptosis. Our results show that disintegration of nucleoli is an early marker of ROSC-induced changes. Cell cycle arrest precedes the main wave of apoptosis.
Wojciechowski
Jacek
J
Cell Cycle Regulation Group, Institute of Cancer Research, University of Vienna, Vienna, Austria.
Horky
Marcel
M
Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
Gueorguieva
Marieta
M
Cell Cycle Regulation Group, Institute of Cancer Research, University of Vienna, Vienna, Austria.
Węsierska-Gądek
Józefa
J
Cell Cycle Regulation Group, Institute of Cancer Research, University of Vienna, Vienna, Austria.
D000107:Acetylation; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D020169:Caspases / Q000378:metabolism*; D002453:Cell Cycle / Q000187:drug effects*; D002458:Cell Fractionation; D002466:Cell Nucleolus / Q000187:drug effects / Q000378:metabolism; D048429:Cell Size / Q000187:drug effects; D018844:Cyclin-Dependent Kinases / Q000037:antagonists & inhibitors; D004789:Enzyme Activation; D006657:Histones / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D009363:Neoplasm Proteins / Q000378:metabolism; D009697:Nucleolus Organizer Region / Q000473:pathology*; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D011687:Purines / Q000494:pharmacology*; D016601:RNA-Binding Proteins / Q000378:metabolism; D000077546:Roscovitine; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D000097796:Nucleolin
D000970:Antineoplastic Agents; D020169:Caspases; D018844:Cyclin-Dependent Kinases; D006657:Histones; D009363:Neoplasm Proteins; D010750:Phosphoproteins; D011065:Poly(ADP-ribose) Polymerases; D011687:Purines; D016601:RNA-Binding Proteins; D000077546:Roscovitine; D016159:Tumor Suppressor Protein p53; D000097796:Nucleolin