Effects of L-THP on Ca2+ overload of cultured rat cardiomyocytes during hypoxia and reoxygenation.
The effects of L-tetrahydropalmatine (L-THP) on the cultured rat cardiomyocytes during hypoxia and reoxygenation and the mechanism of L-THP treating reperfusion-arrythmias were studied. The concentration of intracellular free calcium ([Ca2+]i) of single cultured ventricular myocyte was determined by using EPC-9 light-electricity measurement system. It was found that L-THP (100 mumol/L) could reduce the [Ca2+]i augmentation in single cultured ventricular myocyte during hypoxia and reoxygenation. Verapamil (10 mumol/L) had the similar effect. It was concluded that L-THP could inhibit the Ca2+ overload of cultured rat cardiomyocytes during hypoxia and reoxygenation.
Zeng
Q
Q
Institute of Cardiovascular Diseases, Tongji Medical University, Wuhan 430022.
Zhu
W
W
Cao
L
L
Liu
F
F
D000818:Animals; D000831:Animals, Newborn; D001600:Berberine Alkaloids / Q000494:pharmacology*; D001693:Biological Transport, Active; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D015687:Cell Hypoxia; D002478:Cells, Cultured; D015428:Myocardial Reperfusion Injury / Q000378:metabolism* / Q000517:prevention & control; D032383:Myocytes, Cardiac / Q000378:metabolism*; D010100:Oxygen / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013237:Stereoisomerism
D001600:Berberine Alkaloids; D002121:Calcium Channel Blockers; C014215:tetrahydropalmatine; D010100:Oxygen; D002118:Calcium