Leukocyte 8-hydroxydeoxyguanosine and aromatic DNA adduct in coke-oven workers with polycyclic aromatic hydrocarbon exposure.
OBJECTIVE
To investigate the potential for exposure to polycyclic aromatic hydrocarbons (PAHs) to induce oxidative DNA damage, we conducted a cross-sectional study in coke-oven workers employed at an iron-steel factory.


METHODS
The study population contained 119 coke-oven workers from different work areas of the oven and 38 controls. Personal information on age, employment duration, smoking habit and alcohol consumption was obtained at an interview. Leukocyte 8-hydroxydeoxyguanosine (8-OHdG) was measured by high performance liquid chromatography with electrochemical detection. Leukocyte aromatic DNA adducts as effective dose, and urinary 1-hydroxypyren as internal dose, were also measured, and used to analyze the relationship of 8-OHdG with other biomarkers for PAH exposure, tobacco smoke and alcohol consumption.


RESULTS
The leukocyte 8-OHdG revealed a wide inter-individual variation. The highest 8-OHdG level was detected in bottom-workers of the coke-oven. There were significant differences among the four different work areas ( P=0.02). We could not find significant correlation between 8-OHdG levels and urinary 1-hydroxypyrene, but a weakly positive correlation was found between 8-OHdG and leukocyte aromatic DNA adducts among all subjects (r=0.19 P=0.03). We could not observe any effect of smoking and alcohol drinking on 8-OHdG production.


CONCLUSIONS
We could not find clear evidence that PAH exposure induces oxidative DNA damage.
Zhang
J
J
Department of Social and Environmental Medicine, Saga Medical School, Japan.
Ichiba
M
M
Hanaoka
T
T
Pan
G
G
Yamano
Y
Y
Hara
K
K
Takahashi
K
K
Tomokuni
K
K
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D002617:Chemical Industry; D003430:Cross-Sectional Studies; D018736:DNA Adducts / Q000032:analysis*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis*; D006801:Humans; D007962:Leukocytes / Q000737:chemistry*; D008297:Male; D016273:Occupational Exposure; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity*
D018736:DNA Adducts; D011084:Polycyclic Aromatic Hydrocarbons; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine