title: Environmental isolates of Aeromonas spp. harboring the cagA-like gene of Helicobacter pylori.
abstract: We investigated the presence of cagA-like gene of Helicobacter pylori in environmental isolates of Aeromonas spp. from different water samples of Calcutta, India, by colony hybridization using a cagA-specific DNA probe and by PCR with cagA-specific primers. Nucleotide sequencing of five PCR products revealed 97 to 98% homology to canonical cagA of H. pylori 26695 as well as to four clinical H. pylori strains from Calcutta. The cagA-like gene of the environmental isolates was unstable in laboratory conditions and tended to be lost upon subculturing.
authors.affiliation: National Institute of Cholera and Enteric Diseases, Beliaghata, Calcutta 700 010, India.
authors.forename: Simanti
authors.initials: S
authors.lastname: Datta
authors.forename: Asis
authors.initials: A
authors.lastname: Khan
authors.forename: Ranjan K
authors.initials: RK
authors.lastname: Nandy
authors.forename: Motiur
authors.initials: M
authors.lastname: Rehman
authors.forename: Sutapa
authors.initials: S
authors.lastname: Sinha
authors.forename: Santanu
authors.initials: S
authors.lastname: Chattopadhyay
authors.forename: Suresh C
authors.initials: SC
authors.lastname: Das
authors.forename: G Balakrish
authors.initials: GB
authors.lastname: Nair
mesh_terms: D000333:Aeromonas / Q000235:genetics* / Q000302:isolation & purification*; D000942:Antigens, Bacterial / Q000737:chemistry / Q000235:genetics*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D005618:Fresh Water / Q000382:microbiology*; D016481:Helicobacter Infections / Q000382:microbiology; D016480:Helicobacter pylori / Q000235:genetics; D006801:Humans; D007194:India; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C080866:cagA protein, Helicobacter pylori