[Primary analysis on protein maps of Helicobacter pylori strains associated with gastric carcinoma].
OBJECTIVE
To identify specific proteins of Helicobacter pylori (H. pylori) that associated with gastric carcinoma.


METHODS
The whole-cell proteins of H. pylori were separated by two-dimensional electrophoresis (2-DE). The protein maps of four H. pylori strains associated with gastric carcinoma and nine strains that isolated from patients with non-gastric carcinoma were then compared by ImageMaster 2D v3.1. MALDI-TOF mass spectrometry was performed to identify the proteins of interest. The proteins were searched by software mascot and identified by peptide fingerprint map.


RESULTS
Three proteins seemed to be associated with gastric carcinoma including acylneuraminate cytidylyltransferase with Mowse score 79 with the sequence coverage of 32%. The other two had no unambiguous protein to match.


CONCLUSIONS
Acylneuraminate cytidylyltransferase seemed to be a specific H. pylori protein associated with the presence of gastric carcinoma. Other two were novel proteins that might be associated with gastric carcinoma. However, the mechanism needs to be explored.
Li
Bo-qing
BQ
Department of Diagnosis, Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Zhang
Jian-zhong
JZ
Zou
Qing-hua
QH
He
Li-hua
LH
Yan
Xiao-mei
XM
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional; D016480:Helicobacter pylori / Q000737:chemistry*; D006801:Humans; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D013274:Stomach Neoplasms / Q000382:microbiology*
D001426:Bacterial Proteins