title: Nucleolytic processing of a protein-bound DNA end by the E. coli SbcCD (MR) complex.
abstract: SbcCD and other Mre11/Rad50 (MR) complexes are implicated in the metabolism of DNA ends. They cleave ends sealed by hairpin structures and have been postulated to play roles in removing protein bound to DNA termini. Here we provide direct evidence that the Escherichia coli MR complex (SbcCD) removes protein from a protein-bound DNA end by inserting a double-strand break (DSB). These observations indicate a more complex biochemical action than has been assumed previously and argue that this class of protein has the potential to play a direct role in deprotecting protein-bound DNA ends in vivo.
authors.affiliation: Institute of Cell and Molecular Biology, University of Edinburgh, Kings Buildings, Edinburgh EH9 3JR, Scotland, UK. John.Connelly@ed.ac.uk
authors.forename: John C
authors.initials: JC
authors.lastname: Connelly
authors.forename: Erica S
authors.initials: ES
authors.lastname: de Leau
authors.forename: David R F
authors.initials: DR
authors.lastname: Leach
mesh_terms: D001360:Avidin; D001426:Bacterial Proteins / Q000235:genetics*; D001710:Biotin; D002855:Chromatography, Thin Layer; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics*; D005090:Exodeoxyribonucleases / Q000235:genetics / Q000378:metabolism*; D005092:Exonucleases / Q000235:genetics*; D008958:Models, Molecular; D009841:Oligonucleotides
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D009841:Oligonucleotides; C061563:SbcC protein, Bacteria; C489229:SbcC protein, E coli; D001360:Avidin; D001710:Biotin; D004247:DNA; D003851:Deoxyribonucleases; D005090:Exodeoxyribonucleases; D005092:Exonucleases; C081229:sbcD protein, E coli