[Sequences analysis of cytochrome C oxidase subunit I gene in Aedes albopictus from different geographic strains in China].
OBJECTIVE
To compare the sequences of cytochrome C oxidase subunit I gene (COI) in Aedes albopictus from different geographic strains in China and to discuss the differences in susceptibility among different geographic strains to dengue virus (DV).


METHODS
COI was amplified with polymerase chain reaction method and sequenced from its genomic DNA. Molecular phylogenetic trees were constructed with Neighbor-Joining method.


RESULTS
Sequence length of COI fragment in each geographic strains was 415 bp. The rates of shift and reverse of base pairs in Simao strain were 1.93% and 0.24% respectively. The rate of shift in Mawei and Nanning strains was 0.48%. The analyses of phylogenetic of COI sequences showed that there was close relationship between Simao strain in Yunnan and Mawei strain in Guizhou and between Mawei strain and Nanning strain in Guangxi.


CONCLUSIONS
The susceptibility was widely related to many factors including genetic and environmental ones. COI in Aedes albopictus from different geographic strains in China belonged to the same gene type. There were no direct correlations between COI gene type in different geographic strains and susceptibility to DV.
Chen
Hong
H
General Hospital of Chinese Peoples' Armed Police Forces, Beijng 100039, China.
Chen
Han-bin
HB
D000330:Aedes / Q000235:genetics* / Q000821:virology*; D000818:Animals; D003715:Dengue / Q000635:transmission*; D003576:Electron Transport Complex IV / Q000235:genetics*; D005838:Genotype; D007303:Insect Vectors; D016133:Polymerase Chain Reaction
D003576:Electron Transport Complex IV