Characterization of an alpha-L-arabinofuranosidase gene (abf1) from Penicillium purpurogenum and its expression.
An alpha-L-arabinofuranosidase gene (abf1) from Penicillium purpurogenum was identified and sequenced. abf1 has an open reading frame of 1518 bp, does not contain introns and codes for a protein of 506 amino acids. The deduced mature protein has a molecular mass of 49.6 KDa, and its sequence is homologous to arabinofuranosidases of glycosyl hydrolase family 54. Southern blots suggest that abf1 is a single copy gene. Putative sequences for the binding of the transcriptional regulators XlnR, CreA, PacC, AlcR and AreA are present in the promoter. Northern-blot analysis shows that abf1 is expressed at neutral but not at alkaline or acidic pH values. The presence of binding sites for regulatory elements in the promoter region has been compared to the genes of other fungal enzymes belonging to the same family. This is the first characterization of an abf gene from a Penicillium species.
Carvallo
Marcela
M
Laboratorio de Bioquimíca, Departamento de Genética Molecular y Microbiología, Pontificia Universidad Católica de Chile, Casilla 114-D, Santiago, Chile.
de Ioannes
Pablo
P
Navarro
Claudio
C
Chavez
Renato
R
Peirano
Alessandra
A
Bull
Paulina
P
Eyzaguirre
Jaime
J
D000595:Amino Acid Sequence; D001665:Binding Sites; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D005809:Genes, Regulator / Q000502:physiology; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames; D010407:Penicillium / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012331:RNA, Fungal / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D016415:Sequence Alignment
D012331:RNA, Fungal; D012333:RNA, Messenger; D006026:Glycoside Hydrolases; C019713:alpha-N-arabinofuranosidase