title: Subcellular distribution of Homer 1b/c in relation to endoplasmic reticulum and plasma membrane proteins in Purkinje neurons.
abstract: The subcellular distribution of endoplasmic reticulum proteins (IP3R1 and RYR), plasma membrane (PM) proteins (mGluR1 and PMCA Ca(2+)-pump), and scaffolding proteins, such as Homer 1b/c, was assessed by laser scanning confocal microscopy of rat cerebellum parasagittal sections. There appeared to be two classes of Ca2+ stores, nonjunctional Ca2+ stores and junctional Ca2+ stores, possibly referable to central cisternae/tubules and sub-PM cisternae, respectively, in soma, dendrites, and dendritic spines. Only some IP3R1s appeared to be part of multimeric, junctional Ca2+ signaling networks, whose composition is shown to include PMCA, mGluR1, Homer 1b/c and, not always, RYR1.
authors.affiliation: Dipartimento di Scienze Biomediche Sperimentali dell'Università degli Studi di Padova, viale G. Colombo 3, 35121 Padova, Italy.
authors.forename: Dorianna
authors.initials: D
authors.lastname: Sandonà
authors.forename: Alessandra
authors.initials: A
authors.lastname: Scolari
authors.forename: Katsuiko
authors.initials: K
authors.lastname: Mikoshiba
authors.forename: Pompeo
authors.initials: P
authors.lastname: Volpe
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D000072233:Homer Scaffolding Proteins; D008565:Membrane Proteins / Q000378:metabolism*; D018613:Microscopy, Confocal; D009474:Neurons / Q000378:metabolism*; D009479:Neuropeptides / Q000737:chemistry / Q000378:metabolism*; D011689:Purkinje Cells / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013347:Subcellular Fractions / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D000072233:Homer Scaffolding Proteins; D008565:Membrane Proteins; D009479:Neuropeptides