Mutagenesis approaches to deduce structure-function relationships in terpene synthases.
This review highlights mutagenesis studies of terpene synthases, specifically sesquiterpene synthases and oxidosqualene cyclases. Mutagenesis studies of these enzymes have provided mechanistic insights, structure-function relationships for specific enzymatic residues, novel terpene structures and enzymes with novel activities. The literature through 2002 is reviewed and 113 references cited.
Segura
Michael J R
MJ
Department of Chemistry, Rice University, Houston, Texas 77005, USA.
Jackson
Beth E
BE
Matsuda
Seiichi P T
SP
D019883:Alkyl and Aryl Transferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D002384:Catalysis; D016296:Mutagenesis; D010944:Plants / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism; D013329:Structure-Activity Relationship; D013729:Terpenes / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism
D013729:Terpenes; D019883:Alkyl and Aryl Transferases; C108877:terpene synthase