Characterization of loaded liposomes by size exclusion chromatography.
This review focuses on the use of conventional (SEC) and high performance (HPSEC) size exclusion chromatography for the analysis of liposomes. The suitability of both techniques is examined regarding the field of liposome applications. The potentiality of conventional SEC is strongly improved by using a HPLC system associated to gel columns with a size selectivity range allowing liposome characterization in addition to particle fractionation. Practical aspects of size exclusion chromatography are described and a methodology based on HPSEC coupled to multidetection modes for on-line analysis of liposomes via label or substance encapsulation is presented. Examples of conventional SEC and HPSEC applications are described which concern polydispersity, size and encapsulation stability, bilayer permeabilization, liposome formation and reconstitution, incorporation of amphiphilic molecules. Size exclusion chromatography is a simple and powerful technique for investigation of encapsulation, insertion/interaction of substances from small solutes (ions, surfactants, drugs, etc.) up to large molecules (proteins, peptides and nucleic acids) in liposomes.
Grabielle-Madelmont
Cécile
C
Equipe Physico-chimie des Systèmes Polyphasés, UMR CNRS 8612, Université Paris-Sud, Châtenay-Malabry Cedex 92296, France. cecile.grabielle-madelmont@cep.u-psud.fr
Lesieur
Sylviane
S
Ollivon
Michel
M
D002850:Chromatography, Gel / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005782:Gels / Q000737:chemistry*; D008081:Liposomes / Q000032:analysis* / Q000737:chemistry*; D008968:Molecular Conformation; D010539:Permeability; D014677:Pharmaceutical Vehicles / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry
D020099:Coated Materials, Biocompatible; D005782:Gels; D008081:Liposomes; D014677:Pharmaceutical Vehicles; D013501:Surface-Active Agents