Promoting effect of kaempferol on the differentiation and mineralization of murine pre-osteoblastic cell line MC3T3-E1.
A number of agents have been reported to influence osteoblastic differentiation and to prevent and treat bone loss. We found that kaempferol, a flavonoid identified in extracts of the medicinal plant, Polygonum tinctorium. Lour, had stimulatory effects on the differentiation and mineralization of the murine pre-osteoblastic cell line, MC3T3-E1. After enhancing the alkaline phosphatase activity, significant augmentation of calcification by kaempferol was observed between concentrations of 10 and 20 microM, without any marked effect on cell proliferation. When kaempferol was combined with ipriflavone, which is clinically applied to treat bone loss, calcification was synergistically augmented, suggesting that these two flavonoids may have different mechanisms of action. These results suggest that kaempferol may be a promising agent for the prevention or treatment of bone loss, especially when combined with ipriflavone.
Miyake
Masaki
M
Fujisaki Institute, Hayashibara Biochemical Laboratories Inc.. 675 Fujisaki, Okayama 702-8006, Japan. fujisaki@hayashibara.co.jp
Arai
Norie
N
Ushio
Shimpei
S
Iwaki
Kanso
K
Ikeda
Masao
M
Kurimoto
Masashi
M
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002113:Calcification, Physiologic / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D007529:Isoflavones / Q000494:pharmacology; D044949:Kaempferols / Q000494:pharmacology*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D013997:Time Factors
D007529:Isoflavones; D044949:Kaempferols; C006552:kaempferol; C018986:ipriflavone; D000469:Alkaline Phosphatase