Covalent attachment of DNA to glass supports using a new silane coupling agent and chemiluminescent detection.
A new kind of silane coupling agent, N-(beta-aminoethyl)-gamma-aminopropyl triethoxysilane, was used for DNA direct attachment on the surfaces of glass supports, then the immobilized DNA was hybridized with horseradish peroxidase (HRP)-labeled probe, and detected by using enhanced chemiluminescent method. In comparison with gamma-aminopropyl triethoxysilane, the detection limits (S/N) of DNA were 10 pg and 75 pg respectively. Several experimental conditions of DNA attach-ING to glass supports were investigated, and the system of hybridization of nucleic acid on the surfaces of glass supports was developed.
Zhang
G
G
Institute of Environmental Medicine, Tongji Medical University, Wuhan 430030.
Zhou
Y
Y
Wu
X
X
Yuan
J
J
Ren
S
S
D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D005898:Glass; D006735:Horseradish Peroxidase; D006801:Humans; D008163:Luminescent Measurements; D009693:Nucleic Acid Hybridization; D012821:Silanes; D012826:Silicone Elastomers
D012821:Silanes; D012826:Silicone Elastomers; C006194:aminoethyl-aminopropyl-trimethoxysilane; D004247:DNA; D006735:Horseradish Peroxidase