The effect of astragapolysaccharide on the lymphocyte proliferation and airway inflammation in sensitized mice.
In order to investigate the regulating role of Astragapolysaccharide (APS) in the mice model of asthmatic airway inflammation, the airway eosinophil number, spleen T lymphocyte proliferation, level of IL-2 production and their relationships were studied in sensitized mice and sensitized mice treated with different concentrations of APS. The results showed that the number of eosinophils as well as lymphocytes in the airway of the sensitized animals were significantly increased, and a marked positive correlation between the inflammation cells and spleen T lymphocyte proliferation was found. Moreover, there was a positive correlation between inflammation cells and the level of IL-2 production. The APS of given dosage could significantly reduce the number of eosinophils in the airway of the sensitized animals. At the same time the level of IL-2 secreted by spleen T lymphocytes stimulated with ConA was also significantly decreased and there was a marked positive correlation between them. Our results suggested that APS of given dosage could prevent antigen-induced the number of eosinophils infiltrating into the airway of sensitized mice and inhibit the proliferation and activation of lymphocyte and IL-2 production. Through its immuno-regulating effect, APS can be helpful in the treatment of asthma.
Xue
J
J
Department of Respiratory Diseases, Tongji Medical University, Wuhan 430030.
Xu
Y
Y
Zhang
Z
Z
Shen
G
G
Zeng
G
G
D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000276:immunology*; D027885:Astragalus propinquus / Q000737:chemistry*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D007376:Interleukin-2 / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin; D011134:Polysaccharides / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000276:immunology
D007376:Interleukin-2; D011134:Polysaccharides; D010047:Ovalbumin