Regulatory mechanism of the gene expression during chlorovirus infection cycle.
In chlorovirus infection, immediate-early gene expression occurs within 5-20 min post infection (p.i.). We identified 16 major immediate-early transcripts. On Northern blot analysis, these transcripts showed unusual behaviors: (I) after 40 min p.i., their sizes reduced by approximately 100 nt, most likely caused by loss of a poly(A) tail and (II) after 40 min p.i., often appeared a few larger additional transcripts that may be formed by read-through from an upstream gene or into a downstream gene. These results indicate that the transcription of the viral genes changes drastically between before and after 40 min p.i.
Kawasaki
T
T
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-4-1 Kagamiyama, Higashi-Hiroshima 739-8527, Japan.
Tanaka
M
M
Nishida
K
K
Yamada
T
T
D002708:Chlorella / Q000821:virology; D015967:Gene Expression Regulation, Viral; D017874:Immediate-Early Proteins / Q000096:biosynthesis / Q000235:genetics; D007700:Kinetics; D017862:Phycodnaviridae / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D012367:RNA, Viral / Q000096:biosynthesis / Q000737:chemistry; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics
D017874:Immediate-Early Proteins; D012367:RNA, Viral; D014764:Viral Proteins