Mapping of Rym14Hb, a gene introgressed from Hordeum bulbosum and conferring resistance to BaMMV and BaYMV in barley.
Hordeum bulbosum represents the secondary gene pool of barley and constitutes a potential source of various disease resistances in barley breeding. Interspecific crosses of H. vulgare x H. bulbosum resulted in recombinant diploid-barley progeny with immunity to BaMMV after mechanical inoculation. Tests on fields contaminated with different viruses demonstrated that resistance was effective against all European viruses of the soil-borne virus complex (BaMMV, BaYMV-1, -2). Genetic analysis revealed that resistance was dominantly inherited. Marker analysis in a F5 mapping family was performed to map the introgression in the barley genome and to estimate its size after several rounds of recombination. RFLP anchor-marker alleles indicative of an H. bulbosum introgression were found to cover an interval 2.9 cM in length on chromosome 6HS. The soil-borne virus resistance locus harboured by this introgressed segment was designated Rym14(Hb). For marker-assisted selection of Rym14(Hb) carriers, a diagnostic codominant STS marker was derived from an AFLP fragment amplified from leaf cDNA of homozygous-resistant genotypes inoculated with BaMMV.
Ruge
B
B
Federal Centre for Breeding Research on Cultivated Plants, Institute of Agricultural Crops, Rudolf-Schick-Platz 3a, D-18190 Gross Lüsewitz, Germany. bafz-lk@bafz.de
Linz
A
A
Pickering
R
R
Proeseler
G
G
Greif
P
P
Wehling
P
P
D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D005819:Genetic Markers; D001467:Hordeum / Q000235:genetics* / Q000378:metabolism* / Q000821:virology; D007113:Immunity, Innate / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D010935:Plant Diseases; D012150:Polymorphism, Restriction Fragment Length
D005819:Genetic Markers