title: Shear-flow-based chromatographic separations as an alternative to pressure-driven liquid chromatography.
abstract: It is only by developing specially designed injection and detection systems that shear-driven chromatography can become a viable alternative to HPLC. In the present paper, a dedicated zero dead-volume injection procedure is presented with which sample volumes can be injected reproducibly in the required picoliter range. In addition, a transversal detection groove system is designed which should allow to perform on-line UV-VIS absorption measurements with path lengths in the millimeter range, with an acceptable theoretical plate loss (only 20% in a 5 cm long channel) and acting as a nearly perfect wave guide.
authors.affiliation: Vrije Universiteit Brussel, Department of Chemical Engineering, Pleinlaan 2, 1050 Brussels, Belgium. gedesmet@vub.ac.be
authors.forename: G
authors.initials: G
authors.lastname: Desmet
authors.forename: N
authors.initials: N
authors.lastname: Vervoort
authors.forename: D
authors.initials: D
authors.lastname: Clicq
authors.forename: A
authors.initials: A
authors.lastname: Huau
authors.forename: P
authors.initials: P
authors.lastname: Gzil
authors.forename: G V
authors.initials: GV
authors.lastname: Baron
mesh_terms: D000465:Algorithms; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods; D004058:Diffusion; D013056:Spectrophotometry, Ultraviolet