Efficient synthesis of globoside and isogloboside tetrasaccharides by using beta(1-->3) N-acetylgalactosaminyltransferase/UDP-N-acetylglucosamine C4 epimerase fusion protein.
The beta(1-->3) N-acetylgalactosaminyltransferase/UDP-N-acetylglucosamine C4 epimerase fusion protein was constructed and used in coupled enzymatic reactions to synthesize a variety of globotetraose and isoglobotetraose derivatives from the corresponding lactoside acceptors.
Shao
Jun
J
Department of Chemistry, Wayne State University, Detroit, MI 48202, USA.
Zhang
Jianbo
J
Kowal
Przemyslaw
P
Lu
Yuquan
Y
Wang
Peng George
PG
D002240:Carbohydrate Sequence; D005915:Globosides / Q000138:chemical synthesis*; D006027:Glycosides / Q000737:chemistry; D008956:Models, Chemical; D008969:Molecular Sequence Data; D017350:N-Acetylgalactosaminyltransferases / Q000737:chemistry*; D009844:Oligosaccharides / Q000138:chemical synthesis*; D004364:Pharmaceutical Preparations; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D014534:UDPglucose 4-Epimerase / Q000737:chemistry*; D014531:Uridine Diphosphate Galactose / Q000737:chemistry
D005915:Globosides; D006027:Glycosides; D009844:Oligosaccharides; D004364:Pharmaceutical Preparations; D011993:Recombinant Fusion Proteins; C008245:lactosides; D014531:Uridine Diphosphate Galactose; C035968:globotetraose; D017350:N-Acetylgalactosaminyltransferases; D014534:UDPglucose 4-Epimerase