title: HLA-B27 subtypes determination in patients with ankylosing spondylitis from Zulia, Venezuela.
abstract: To perform an investigation regarding the distribution of the human leukocyte antigen (HLA)-B27 subtypes in the Zulian population with ankylosing spondylitis (AS), 48 unrelated Mestizos, HLA-B27 positive by serology, were studied using the polymerase chain reaction-specific sequence oligonucleotides probe (PCR-SSOP) and specific sequence primers (SSP) to analyze the polymorphism in exons 2 and 3 of the HLA-B27 gene. Only two of eight HLA-B27 subtypes studied (B*2701-B*2708) were found. The distribution of these alleles in the population of patients was: B*2705, 68.8%, and B*2702, 31.2%. B*2705 subtype showed significant association with patients being male. In the healthy controls, the most common subtype was B*2708. These results were compared with frequencies reported in other Mestizo and Spanish populations and showed significant differences, such as a high frequency of B*2702. Such results show that HLA*B2705 and HLA*B2702 are the subtypes most frequently associated with AS in our Mestizo population and suggest a possible protector role for HLA*B2708, which was found only in the healthy population.
authors.affiliation: University of Zulia, Zulia, Venezuela.
authors.forename: Ana
authors.initials: A
authors.lastname: Cipriani
authors.forename: Sergio
authors.initials: S
authors.lastname: Rivera
authors.forename: Manzur
authors.initials: M
authors.lastname: Hassanhi
authors.forename: Georgina
authors.initials: G
authors.lastname: Márquez
authors.forename: Rosaura
authors.initials: R
authors.lastname: Hernández
authors.forename: Cecilia
authors.initials: C
authors.lastname: Villalobos
authors.forename: Milagros
authors.initials: M
authors.lastname: Montiel
mesh_terms: D000293:Adolescent; D000328:Adult; D000375:Aging; D000483:Alleles; D044383:Black People; D017931:DNA Primers; D005006:Ethnicity / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D015796:HLA-B27 Antigen / Q000032:analysis / Q000145:classification / Q000235:genetics*; D015395:Histocompatibility Antigens Class I; D006801:Humans; D007199:Indians, South American / Q000235:genetics; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012727:Sex Characteristics; D013167:Spondylitis, Ankylosing / Q000453:epidemiology / Q000235:genetics*; D014606:Uveitis, Anterior / Q000453:epidemiology / Q000235:genetics; D014687:Venezuela / Q000453:epidemiology; D044465:White People
chemical_list: D017931:DNA Primers; D015796:HLA-B27 Antigen; D015395:Histocompatibility Antigens Class I