title: Adjustment function among antioxidant substances in acatalasemic mouse brain and its enhancement by low-dose X-ray irradiation.
abstract: The catalase activities in blood and organs of the acatalasemic (C3H/AnLCsbCsb) mouse of the C3H strain are lower than those of the normal (C3H/AnLCsaCsa) mouse. We conducted a study to examine changes in the activities of antioxidant enzymes, such as catalase, superoxide dismutase (SOD) and glutathione peroxidase (GPX), the total gluathione content, and the lipid peroxide level in the brain, which is more sensitive to oxidative stress than other organs, at 3, 6, or 24 hr following X-ray irradiation at doses of 0.25, 0.5, or 5.0 Gy to the acatalasemic and the normal mice. No significant change in the lipid peroxide level in the acatalasemic mouse brain was seen under non-irradiation conditions. However, the acatalasemic mouse brain was more damaged than the normal mouse brain by excessive oxygen stress, such as a high-dose (5.0 Gy) X-ray. On the other hand, we found that, unlike 5.0 Gy X-ray, a relatively low-dose (0.5 Gy) irradiation specifically increased the activities of both catalase and GPX in the acatalasemic mouse brain making the activities closer to those in the normal mouse brain. These findings may indicate that the free radical reaction induced by the lack of catalase is more properly neutralized by low dose irradiation.
authors.affiliation: Medical Radioscience, Okayama University Medical School, 2-5-1 Shikata, Okayama-shi, Okayama 700-8558, Japan.
authors.forename: Kiyonori
authors.initials: K
authors.lastname: Yamaoka
authors.forename: Takaharu
authors.initials: T
authors.lastname: Nomura
authors.forename: Da-Hong
authors.initials: DH
authors.lastname: Wang
authors.forename: Shuji
authors.initials: S
authors.lastname: Mori
authors.forename: Takehito
authors.initials: T
authors.lastname: Taguchi
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Ishikawa
authors.forename: Katsumi
authors.initials: K
authors.lastname: Hanamoto
authors.forename: Shohei
authors.initials: S
authors.lastname: Kira
mesh_terms: D020642:Acatalasia / Q000378:metabolism*; D000222:Adaptation, Physiological / Q000502:physiology; D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism; D001921:Brain / Q000201:enzymology / Q000378:metabolism* / Q000528:radiation effects*; D002374:Catalase; D004307:Dose-Response Relationship, Radiation; D004789:Enzyme Activation / Q000528:radiation effects; D005978:Glutathione / Q000032:analysis / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000032:analysis / Q000378:metabolism; D008054:Lipid Peroxides / Q000032:analysis / Q000378:metabolism; D051379:Mice; D012016:Reference Values; D013045:Species Specificity; D013482:Superoxide Dismutase / Q000032:analysis / Q000378:metabolism; D014916:Whole-Body Irradiation
chemical_list: D000975:Antioxidants; D008054:Lipid Peroxides; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione