Hemocytes of the centipede Scutigera coleoptrata (Chilopoda, Notostigmophora) with notes on their interactions with the tracheae.
The hemocytes of Scutigera coleoptrata were investigated by light and electron microscopy. Four types of hemocytes were identified: prohemocytes, plasmatocytes, granulocytes, and spherulocytes. Only granulocytes could be distinguished from the three other types by May-Grünwald staining, as this is the only hemocyte type demonstrating an eosinophilic reaction. Shape and size give further indications for distinguishing the cell types. In addition, differentiation is possible on the basis of their ultrastructure. However, only a combination of all three methods (staining and light and electron microscopy) allows clear separation of the cell types. As transitional stages between the cell types occur in S. coleoptrata, it is likely that prohemocytes, plasmatocytes, and granulocytes are ontogenetic stages of a single cell lineage. Special cell components and their possible functions are described. Plasmatocytes exocytose tubular structures that probably play a role in coagulation processes. These tubular structures develop in the grana of plasmatocytes. Also, a special arrangement of microtubules and microfilaments was demonstrated. For the first time interactions between hemocytes and tracheae are documented within the Chilopoda. It is assumed that the hemocytes meet their oxygen requirements directly from the tracheae. Phylogenetic implications of the results are discussed.
Hilken
Gero
G
Zentrales Tierlaboratorium Universitätsklinikum Essen, D-45122 Essen, Germany. gero.hilken@uni-essen.de
Brockmann
Claudia
C
Nevermann
Lutz
L
D000818:Animals; D006434:Hemocytes / Q000648:ultrastructure*; D006652:Histological Techniques; D007313:Insecta / Q000502:physiology / Q000648:ultrastructure*; D008854:Microscopy, Electron; D010802:Phylogeny; D014132:Trachea / Q000502:physiology