Comparison between two warm ischemic models in experimental liver transplantation in pigs.
BACKGROUND
Experimental models of warm ischemia in liver transplantation have been employed to study the mechanisms and treatment of ischemia reperfusion injury.


METHODS
We compared a control group without (group A, n = 10) versus two models of warm ischemia of liver transplants in pigs: namely, occlusion of the hepatic artery and portal vein for 30 minutes (group B, n = 23) and extraction of the liver 60 minutes after cardiac arrest (group C, n = 5). Liver function tests, coagulation studies, and liver biopsies were performed during the first 24 hours post-liver transplant.


RESULTS
Clamping of the hepatic vasculature in group B produced a significant liver injury compared with the control group: elevation of the ALT and an abnormal 1-hour post-revascularization biopsy similar to that observed in the cardiac arrest group C. The transaminase levels were lower among group A animals (P <.05). But the hepatic synthetic functions as reflected in the protrombin time (PT) were not affected in group B versus group A. The alteration in PT with respect to the initial value was similar among group A and group B animals, which were significantly less than that in group C (P <.05).


CONCLUSIONS
Occlusion of the hepatic artery and portal vein, a simple surgical maneuver, causes moderate damage to a liver graft but less alteration of hepatic synthetic function. Clamping of the hepatic vasculture obtains more long-term survivors after OLT than cardiac arrest.
Regueira
F M
FM
General Surgery Department, University Clinic of Navarra, Pamplona, Spain. mregueira@unav.es
Espí
A
A
Nwose
P
P
Díez-Caballero
A
A
Baixaulí
J
J
Rotellar
F
F
Olea
J
J
Pardo
F
F
Hernández-Lizoain
J L
JL
Cienfuegos
J A
JA
D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D007511:Ischemia; D008099:Liver / Q000166:cytology / Q000473:pathology; D008102:Liver Circulation; D016031:Liver Transplantation / Q000502:physiology*; D023421:Models, Animal; D009926:Organ Preservation / Q000379:methods; D011516:Prothrombin / Q000378:metabolism; D011517:Prothrombin Time; D013552:Swine; D013997:Time Factors; D014184:Transplantation, Homologous
D011516:Prothrombin; D001219:Aspartate Aminotransferases