title: Mutation analysis of copper-transporting P-type adenosine triphosphatase (ATP7B) in human solid carcinomas.
abstract: A major obstacle in the treatment of human solid carcinomas is the intrinsic/acquired resistance to cisplatin-based chemotherapy. Copper-transporting P-type adenosine triphosphatase (ATP7B) has been reported to be associated with cisplatin resistance in vitro. ATP7B is overexpressed in human solid carcinomas such as breast, gastric and oral squamous cell carcinomas. ATP7B expression has an influential effect on some subsets of patients with cisplatin-treated carcinomas. ATP7B mutation is well-known as a cause of Wilson's disease. In addition, the six copper-binding domain and ATP-binding domain of ATP7B are important for the transportation of metals. Therefore, we performed the mutation analysis at the six copper-binding domain and ATP-binding domain of ATP7B. No mutation at the six copper-binding domain and ATP-binding domain was observed in breast, gastric and oral squameous cell carcinomas. These results indicate that the analysis of the ATP7B gene and/or protein will be helpful for the choice of chemotherapy in patients with human solid carcinomas.
authors.affiliation: Department of Pathology, Institute of Development, Aging and Cancer, Tohoky University, Sendai, Japan.
authors.forename: Atsuko
authors.initials: A
authors.lastname: Kanzaki
authors.forename: Kentaro
authors.initials: K
authors.lastname: Nakayama
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Miyashita
authors.forename: Shinobu
authors.initials: S
authors.lastname: Shirata
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Nitta
authors.forename: Masahide
authors.initials: M
authors.lastname: Oubu
authors.forename: Masashi
authors.initials: M
authors.lastname: Higashimoto
authors.forename: Masato
authors.initials: M
authors.lastname: Mutoh
authors.forename: Shiro
authors.initials: S
authors.lastname: Mori
authors.forename: Soichi
authors.initials: S
authors.lastname: Konno
authors.forename: Kenji
authors.initials: K
authors.lastname: Ogawa
authors.forename: Masakazu
authors.initials: M
authors.lastname: Toi
authors.forename: Yuji
authors.initials: Y
authors.lastname: Takebayashi
mesh_terms: D000251:Adenosine Triphosphatases / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D001943:Breast Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism; D027682:Cation Transport Proteins / Q000096:biosynthesis / Q000235:genetics*; D003300:Copper / Q000378:metabolism; D000073840:Copper-Transporting ATPases; D004252:DNA Mutational Analysis; D019008:Drug Resistance, Neoplasm; D006801:Humans; D009062:Mouth Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism; D009369:Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism
chemical_list: D027682:Cation Transport Proteins; D003300:Copper; D000251:Adenosine Triphosphatases; C000612464:ATP7B protein, human; D000073840:Copper-Transporting ATPases