Identification of the inositol isomers present in Tetrahymena.
The inositol isomer composition of phosphoinositides, polyphosphoinositols, phosphatidylinositol-linked glycans, and glycosyl phosphatidylinositol-anchored proteins of logarithmic phase Tetrahymena vorax was determined by GC-MS analysis of trimethylsilylimadazole derivatives. The most abundant inositol found was the myo-isomer; however, appreciable percentages of scylloinositol were present in the free inositol pool, phosphatidylinositol-linked glycan fraction, and glycosyl phosphatidylinositol-anchored protein fraction. Trace quantities of chiro- and neo-inositols also were present.
Kersting
Michael C
MC
Department of Biochemistry and Molecular Biology, Mississippi State University, Mississippi State, Mississippi 39762, USA.
Boyette
Michele
M
Massey
Joseph H
JH
Ryals
Phillip E
PE
D000818:Animals; D002478:Cells, Cultured; D017261:Glycosylphosphatidylinositols / Q000302:isolation & purification / Q000378:metabolism; D007294:Inositol / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D007295:Inositol Phosphates / Q000302:isolation & purification / Q000378:metabolism; D007536:Isomerism; D008055:Lipids / Q000302:isolation & purification; D013768:Tetrahymena / Q000737:chemistry*
D017261:Glycosylphosphatidylinositols; D007295:Inositol Phosphates; D008055:Lipids; D007294:Inositol