title: [Development of a new biosensor for biochemical oxygen demand].
abstract: OBJECTIVE
  To use a new kind of fixing material, i.e. Sol-Gel organic-inorganic hybridized material to immobilize bacterium to detect Biochemical oxygen demand quickly.
  
  
  METHODS
  The biosensor was fabricated using a thin film in which Hansenula anomala was immobilized by sol-gel and an oxygen electrode. The optimum measurement for biochemical oxygen demand was at pH 7.0; 28 degrees C; response time 3 - 12 min. Pure organic compound, sewage and rate of recovery were detected with the biosensor.
  
  
  RESULTS
  It shows that the BOD biosensor can be used to detect many organic compounds such as amino acid, glucide. It is suitable to monitor sewage and industrial waste water which has low level alcohols and phenols. The microbial membrane can work 3 months and remain its 70% activity. It is measured that the rate of recovery of BOD is between 90% to 105% in sewage.
  
  
  CONCLUSIONS
  The study confirmed the effectiveness and usefulness of BOD sensor, which is quick, convenient, low cost and reliable with little interference.
authors.affiliation: The First Hospital of Harbin Medical University, Harbin 150001, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Chang
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Du
authors.forename: Hong-zhi
authors.initials: HZ
authors.lastname: Pan
authors.forename: Jian-bo
authors.initials: JB
authors.lastname: Jia
authors.forename: Bing-quan
authors.initials: BQ
authors.lastname: Wang
authors.forename: Guang-jin
authors.initials: GJ
authors.lastname: Cheng
authors.forename: Shao-jun
authors.initials: SJ
authors.lastname: Dong
mesh_terms: D001419:Bacteria; D015374:Biosensing Techniques / Q000295:instrumentation*; D018914:Cells, Immobilized; D005782:Gels; D008567:Membranes, Artificial; D009757:Nylons; D010100:Oxygen / Q000032:analysis*; D012722:Sewage / Q000032:analysis / Q000382:microbiology
chemical_list: D005782:Gels; D008567:Membranes, Artificial; D009757:Nylons; D012722:Sewage; D010100:Oxygen