title: In vitro glutamate-stimulated release of dopamine from nucleus accumbens core and shell of spontaneously hypertensive rats.
abstract: Spontaneously hypertensive rats (SHR) are used as a model for attention-deficit hyperactivity disorder (ADHD) since SHR display the major symptoms of ADHD (hyperactivity, impulsivity, inablity to sustain attention during behavioral tasks). We previously showed that electrical and/or K+-stimulated release of dopamine (DA) from nerve terminals in the prefrontal cortex, nucleus accumbens, and caudate-putamen of SHR was significantly lower than that of Wistar-Kyoto (WKY) control rats. The aim of the present investigation was to determine whether glutamate-stimulated release of DA from nucleus accumbens core and shell of SHR was significantly different from that of WKY. Using an in vitro superfusion technique, we showed that glutamate-stimulated release of [3H]DA from striatal slices is mediated by glutamate activation of AMPA receptors and that glutamate-stimulated release of [3H]DA from nucleus accumbens core and shell of 4-6-week-old SHR and WKY is not significantly different. Glutamate-stimulated release of [3H]DA from SHR shell is significantly lower than SHR core and there is also a tendency for glutamate-stimulated [3H]DA release from SHR shell to be lower than release from WKY shell.
authors.affiliation: Department of Human Biology, Faculty of Health Sciences, University of Cape Town, Observatory 7925, South Africa. russell@curie.uct.ac.za
authors.forename: Vivienne Ann
authors.initials: VA
authors.lastname: Russell
mesh_terms: D018750:6-Cyano-7-nitroquinoxaline-2,3-dione / Q000494:pharmacology; D000818:Animals; D004298:Dopamine / Q000378:metabolism*; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D018698:Glutamic Acid / Q000494:pharmacology*; D006973:Hypertension / Q000235:genetics / Q000378:metabolism*; D066298:In Vitro Techniques; D009714:Nucleus Accumbens / Q000187:drug effects / Q000378:metabolism*; D010477:Perfusion; D011810:Quinoxalines / Q000494:pharmacology; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D018091:Receptors, AMPA / Q000819:agonists
chemical_list: D018691:Excitatory Amino Acid Antagonists; D011810:Quinoxalines; D018091:Receptors, AMPA; C062865:2,3-dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline; D018698:Glutamic Acid; D018750:6-Cyano-7-nitroquinoxaline-2,3-dione; D004298:Dopamine