Identification of a repressor of late gene expression within the AcMNPV genome.
Viral replication and late gene expression of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) requires a series of late expression factors. We examined the AcMNPV genome for additional factors which affect late gene expression. During these investigations, a segment of the AcMNPV genome located immediately downstream to LEF-7 was found to possess a repressor activity. Elimination of this region resulted in greatly enhanced late gene expression. We were unable to link repressor activity to a single open reading frame, suggesting that a cis-acting element rather than a trans-acting factor is responsible for this phenomenon.
Hefferon
K L
KL
Department of Entomology, University of Georgia, USA. klh22@cornell.edu
D000818:Animals; D002460:Cell Line; D015967:Gene Expression Regulation, Viral; D016679:Genome, Viral; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D018411:Spodoptera / Q000821:virology; D014162:Transfection; D014760:Viral Fusion Proteins / Q000737:chemistry / Q000235:genetics*
D012097:Repressor Proteins; D014760:Viral Fusion Proteins