title: Identification of a distinct class of cytoskeleton-associated mRNAs using microarray technology.
abstract: BACKGROUND
  Interactions between mRNA and the cytoskeleton are critical for the localization of a number of transcripts in eukaryotic somatic cells. To characterize additional transcripts that may be subject to this form of regulation, we developed a two-step approach that utilizes biochemical fractionation of cells to isolate transcripts from different subcellular compartments followed by microarray analysis to examine and compare these subpopulations of transcripts in a massively-parallel manner.
  
  
  RESULTS
  Using this approach, mRNA was extracted from the cytoskeleton-rich and the cytosolic fractions of the promyelocytic HL-60 cell line. We identify a subset of 22 transcripts that are significantly enriched in the cytoskeleton-associated population. The majority of these encode structural proteins and/or proteins known to interact with elements of the cytoskeleton. Localization required an intact actin cytoskeleton and was largely conserved upon differentiation of precursor HL-60 cells to a macrophage-like phenotype.
  
  
  CONCLUSIONS
  We conclude that the association of transcripts with the actin cytoskeleton in somatic cells may be a critical post-transcriptional regulatory event that controls a larger class of genes than has previously been recognized.
authors.affiliation: Vascular Biology Program, Department of Pathology, Harvard Medical School and Children's Hospital, Enders 1007, 300 Longwood Ave, Boston, MA 02115, USA. amybrock@fas.harvard.edu
authors.forename: Amy
authors.initials: A
authors.lastname: Brock
authors.forename: Sui
authors.initials: S
authors.lastname: Huang
authors.forename: Donald E
authors.initials: DE
authors.lastname: Ingber
mesh_terms: D002458:Cell Fractionation / Q000379:methods; D045744:Cell Line, Tumor; D003599:Cytoskeleton / Q000145:classification* / Q000235:genetics; D003600:Cytosol / Q000737:chemistry; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D052138:Genes, Neoplasm; D018922:HL-60 Cells / Q000737:chemistry; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012333:RNA, Messenger / Q000145:classification* / Q000235:genetics
chemical_list: D012333:RNA, Messenger