Comparative analysis for expressed genes by polymerase chain reaction using module-shuffling primers.
We have developed a method for comparative analysis of gene expression. It is based on competitive PCR amplification with module-shuffling primers, followed by gel electrophoresis in a fluorescent DNA sequencer. In this method, tagged-cDNA restriction fragments derived from different sources were amplified in one tube at the same amplification efficiency. The method can detect different amounts of each expressed gene, up to difference in amounts of 30%. The method was successfully used for comparative analysis of expressed genes in yeast.
Uematsu
C
C
Biosystems Research Department, Central Research Laboratory, Hitachi Ltd.
Nishida
J
J
Okano
K
K
Kambara
H
H
Miura
F
F
Ito
T
T
Sakaki
Y
Y
D017931:DNA Primers; D032621:DNA Shuffling; D015870:Gene Expression; D016133:Polymerase Chain Reaction / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism
D017931:DNA Primers