title: [Expression of nuclear factor-kappaB in bone marrow cells from patients with acute leukemia and its relationship with P21, MMP-2 and MMP-9].
abstract: To investigate the expression of NF-kappaB in acute leukemia and its relationship with P21, and matrix metalloproteinases (MMP), the expression of NF-kappaB, P21, MMP-2 and MMP-9 in bone marrow cells from patients with acute leukemia (AL) was detected using immunocytochemical technique. The results showed that the expression ratios of NF-kappaB, P21, MMP-2 and MMP-9 in untreated AL group were significantly higher than those in remission and normal control groups (P < 0.05), and no obvious difference was seen between remission and normal control groups. The expression of NF-kappaB was correlated with that of P21, MMP-2 and MMP-9 (r = 0.767, 0.729 and 0.803, respectively, P < 0.05). This study indicated that P21 protein, encoded by oncogene Ras, and NF-kappaB were super-expressed in leukemia cells. In conclusion, after activation by Ras, NF-kappaB combined with the kappaB sequences of MMP-2 and MMP-9 genes, then upregulated their expression. MMP might enhance the degradative function of leukemic cell, thus to make cells easier to cross through the bone marrow barrier and release into blood.
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Hu
authors.forename: Hui
authors.initials: H
authors.lastname: Sun
authors.forename: Dian-Bin
authors.initials: DB
authors.lastname: Zou
authors.forename: Ling
authors.initials: L
authors.lastname: Sun
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.forename: Ying-Lan
authors.initials: YL
authors.lastname: Jia
mesh_terms: D000208:Acute Disease; D000328:Adult; D001854:Bone Marrow Cells / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007938:Leukemia / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis*; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis*; D016328:NF-kappa B / Q000096:biosynthesis*; D016283:Proto-Oncogene Proteins p21(ras) / Q000096:biosynthesis*; D012074:Remission Induction
chemical_list: D016328:NF-kappa B; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)