Functional genomics guided with MR imaging: mouse tumor model study.
To gain a better understanding of gene expression patterns in tumors, the authors used contrast material-enhanced magnetic resonance (MR) imaging to noninvasively characterize regions within the same tumor to provide a correlate for genomic analysis. Gene expression profiles of samples from a mouse tumor model obtained from contrast-enhanced and nonenhanced regions within the same tumor were compared with MR imaging and functional genomics. From these samples, 11000 genes were analyzed: 10 genes were up-regulated in the contrast-enhanced areas, and one gene was up-regulated in the nonenhanced regions. Several of these genes encode extracellular matrix proteins. Findings in this study demonstrate that MR imaging can serve as a powerful noninvasive tool for characterizing different regions of tumors to guide genomic analysis with high spatial and temporal resolution.
Guccione
Samira
S
Department of Radiology, Stanford University School of Medicine, 1201 Welch Rd, P260, Stanford, CA 94305-5488, USA.
Yang
Yi-Shan
YS
Shi
Gongyi
G
Lee
Daniel Y
DY
Li
King C P
KC
Bednarski
Mark D
MD
D000818:Animals; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D003287:Contrast Media; D018450:Disease Progression; D019786:Gadolinium DTPA; D020869:Gene Expression Profiling; D023281:Genomics; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D003287:Contrast Media; D019786:Gadolinium DTPA