title: A novel sheathed surface organelle of the Helicobacter pylori cag type IV secretion system.
abstract: Type I strains of Helicobacter pylori (Hp) use a type IV secretion system (T4SS), encoded by the cag pathogenicity island (cag-PAI), to deliver the bacterial protein CagA into eukaryotic cells and to induce interleukin-8 secretion. Translocated CagA is activated by tyrosine phosphorylation involving Src-family kinases. The mechanism and structural basis for type IV protein secretion is not well understood. We describe here, by confocal laser scanning microscopy and field emission scanning electron microscopy, a novel filamentous surface organelle which is part of the Hp T4SS. The organelle is often located at one bacterial pole but can be induced by cell contact also along the lateral side of the bacteria. It consists of a rigid needle, covered focally or completely by HP0527 (Cag7 or CagY), a VirB10-homologous protein. HP0527 is also clustered in the outer membrane. The VirB7-homologous protein HP0532 is found at the base of this organelle. These observations demonstrate for the first time by microscopic techniques a complex T4SS-associated, sheathed surface organelle reminiscent to the needle structures of bacterial type III secretion systems.
authors.affiliation: Max von Pettenkofer Institut für Hygiene and Medizinische Mikrobiologie, Ludwig-Maximilians Universität München, D-80336 München, Germany.
authors.forename: Manfred
authors.initials: M
authors.lastname: Rohde
authors.forename: Jürgen
authors.initials: J
authors.lastname: Püls
authors.forename: Renate
authors.initials: R
authors.lastname: Buhrdorf
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Fischer
authors.forename: Rainer
authors.initials: R
authors.lastname: Haas
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics / Q000378:metabolism*; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport; D021961:Cell Membrane Structures / Q000378:metabolism* / Q000648:ultrastructure; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D044404:Genomic Islands; D016480:Helicobacter pylori / Q000378:metabolism* / Q000472:pathogenicity / Q000648:ultrastructure*; D007150:Immunohistochemistry; D008854:Microscopy, Electron / Q000379:methods; D011993:Recombinant Fusion Proteins / Q000378:metabolism
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D011993:Recombinant Fusion Proteins; C080866:cagA protein, Helicobacter pylori