Familial footpad hyperkeratosis and inheritance of keratin 2, keratin 9, and desmoglein 1 in two pedigrees of Irish Terriers.
OBJECTIVE
To investigate the possibility that variants in the acidic or basic keratin genes or in desmoglein 1 may cause the clinical manifestation of familial footpad hyperkeratosis in Irish Terriers.


METHODS
11 dogs belonging to 2 related affected pedigrees of Irish Terriers.


METHODS
Genomic DNA was extracted from blood samples obtained from each dog. The DNA markers linked to the genes keratin 2, keratin 9, and desmoglein 1 were amplified by use of a polymerase chain reaction technique, and length of the products was determined by use of an automatic DNA analyzer.


RESULTS
All tested markers yielded information. None of the markers (genotype) cosegregated with the clinical status of the dogs (phenotype) in the 2 pedigrees.


CONCLUSIONS
Mutations in the genes encoding keratin 2 and 9 as well as desmoglein 1 are highly unlikely to be the primary cause of familial footpad hyperkeratosis in Irish Terriers.
Schleifer
Sebastian G
SG
Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, University of Utrecht, PO Box 80.157, 3508 TD Utrecht, The Netherlands.
Versteeg
Serge A
SA
van Oost
Bernard
B
Willemse
Ton
T
D000818:Animals; D015820:Cadherins / Q000235:genetics*; D004252:DNA Mutational Analysis; D051183:Desmoglein 1; D004283:Dog Diseases / Q000235:genetics*; D004285:Dogs; D005260:Female; D005528:Foot / Q000473:pathology; D005532:Foot Deformities, Congenital / Q000235:genetics; D005819:Genetic Markers; D007633:Keratins / Q000235:genetics*; D008297:Male; D010375:Pedigree; D012868:Skin Abnormalities / Q000235:genetics* / Q000662:veterinary*
D015820:Cadherins; D051183:Desmoglein 1; D005819:Genetic Markers; D007633:Keratins