Structure and chromosomal localization of the murine coxsackievirus and adenovirus receptor gene.
We analyzed BAC genomic clones encoding the murine coxsackievirus and adenovirus receptor (mCAR). The mCAR gene is situated on the distal portion of murine chromosome 16, and is composed of at least eight exons, with intron-exon boundaries similar to those reported for the human CAR gene. We previously described two cDNAs encoding mCAR isoforms: the extracellular and transmembrane portions of both are encoded by exons 1-6; the cytoplasmic domain of mCAR 1 is encoded by exon 7, whereas mCAR 2 results from an RNA splice linking the proximal portion of exon 7 to an alternative exon 8. RT-PCR analysis of the mCAR RNA 5'-terminus suggests that transcription may begin 141-161 nucleotides upstream of the ATG translational start site.
Chen
Jin-Wen
JW
Division of Immunologic & Infectious Diseases, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania 19104, USA.
Ghosh
Ruma
R
Finberg
Robert W
RW
Bergelson
Jeffrey M
JM
D024506:5' Flanking Region; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D022202:Chromosomes, Artificial, Bacterial; D003001:Cloning, Molecular; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D005091:Exons; D006801:Humans; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D016133:Polymerase Chain Reaction / Q000379:methods; D014176:Protein Biosynthesis; D017434:Protein Structure, Tertiary; D011991:Receptors, Virus / Q000235:genetics* / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D024363:Transcription Initiation Site
C568771:CLMP protein, human; C568772:CLMP protein, mouse; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D011991:Receptors, Virus