Effects of fibroblast growth factor 8, fibroblast growth factor 9 and keratinocyte growth factor on multiplication and locomotion in human teratocarcinoma cells in vitro.
BACKGROUND
The effects of three members of the heparin binding growth factor family, FGF-8, FGF-9 and KGF, on the human embryonal carcinoma-derived cells line tera 2 were examined.


METHODS
Cell numbers as well as cell migration was examined at clonal level by microscopy.


RESULTS
It was found that each of these factors promoted Tera 2 cell multiplication, albeit with different efficacy. Whereas a dramatic effect on cell numbers was observed after the addition of 1-10 micrograms FGF-9/ml, a lesser effect could be achieved by FGF-8 or KGF. In contrast, KGF expressed the most potent effect on cell locomotion at a higher concentration (100 micrograms/ml). Even though high concentrations of FGF-8 and FGF-9 did stimulate cell locomotion, this effect was substantially smaller than that of KGF.


CONCLUSIONS
All three FGFs exert a dual effect on Tera 2 cell survival and locomotion.
Granerus
Marika
M
Department of Pathology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, PO Box 7028, 75007 Uppsala, Sweden.
Engström
Wilhelm
W
D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D051523:Fibroblast Growth Factor 7; D051524:Fibroblast Growth Factor 8; D051525:Fibroblast Growth Factor 9; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D006493:Heparin / Q000378:metabolism; D006801:Humans; D011994:Recombinant Proteins / Q000494:pharmacology; D018243:Teratocarcinoma / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects
D016895:Culture Media, Serum-Free; C496453:FGF7 protein, human; C496456:FGF8 protein, human; C496459:FGF9 protein, human; D051525:Fibroblast Growth Factor 9; D011994:Recombinant Proteins; D051523:Fibroblast Growth Factor 7; D051524:Fibroblast Growth Factor 8; D005346:Fibroblast Growth Factors; D006493:Heparin