title: Cks1-dependent proteasome recruitment and activation of CDC20 transcription in budding yeast.
abstract: Cks proteins are small evolutionarily conserved proteins that interact genetically and physically with cyclin-dependent kinases. However, in spite of a large body of genetic, biochemical and structural research, no compelling unifying model of their functions has emerged. Here we show, by investigating the essential role of Cks1 in Saccharomyces cerevisiae, that the protein is primarily involved in promoting mitosis by modulating the transcriptional activation of the APC/C protein-ubiquitin ligase activator Cdc20. Cks1 is required for both the periodic dissociation of Cdc28 kinase from the CDC20 promoter and the periodic association of the proteasome with the promoter. We propose that the essential role of Cks1 is to recruit the proteasome to, and/or dissociate the Cdc28 kinase from, the CDC20 promoter, thus facilitating transcription by remodelling transcriptional complexes or chromatin associated with the CDC20 gene.
authors.affiliation: The Scripps Research Institute, Department of Molecular Biology, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
authors.forename: May C
authors.initials: MC
authors.lastname: Morris
authors.forename: Peter
authors.initials: P
authors.lastname: Kaiser
authors.forename: Stanislav
authors.initials: S
authors.lastname: Rudyak
authors.forename: Chris
authors.initials: C
authors.lastname: Baskerville
authors.forename: Mark H
authors.initials: MH
authors.lastname: Watson
authors.forename: Steven I
authors.initials: SI
authors.lastname: Reed
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D017489:CDC28 Protein Kinase, S cerevisiae / Q000378:metabolism; D064199:Cdc20 Proteins; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000502:physiology*; D003546:Cysteine Endopeptidases / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal; D009097:Multienzyme Complexes / Q000502:physiology*; D011401:Promoter Regions, Genetic; D046988:Proteasome Endopeptidase Complex; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000502:physiology*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C577495:CDC20 protein, S cerevisiae; C081185:CKS1 protein, S cerevisiae; D064199:Cdc20 Proteins; D018797:Cell Cycle Proteins; D009097:Multienzyme Complexes; D029701:Saccharomyces cerevisiae Proteins; D017489:CDC28 Protein Kinase, S cerevisiae; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex