Site of functional interaction of release factor 1 with the ribosome.
Ribosomal protein L11 consists of a C-terminal and an N-terminal domain. To determine the importance of each domain for interaction with release factor 1, which works specifically at the UAG termination codon, we constructed Escherichia coli strains lacking either the entire L11 protein or just the N-terminal portion. Strains lacking L11 exhibited UAG suppression, defective growth, and high-temperature lethality, phenotypes that were reversed by expression of L11 protein from a plasmid. Strains lacking only the N-terminal portion of L11 grew well at physiological temperatures and survived at high temperature, but they were defective in UAG-dependent termination. Our results show for the first time that it is precisely the N-terminal part of ribosomal protein L11 that is required for the functional interaction of release factor 1 with the ribosome in the cell.
Van Dyke
Natalya
N
Department of Molecular Genetics, The University of Texas M. D. Anderson Cancer Center, Box 11, 1515 Holcombe Boulevard, Houston 77030-4009, USA.
Murgola
Emanuel J
EJ
D001665:Binding Sites / Q000502:physiology; D002455:Cell Division / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D010454:Peptide Termination Factors / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*; D013489:Suppression, Genetic; D013696:Temperature
D029968:Escherichia coli Proteins; D010454:Peptide Termination Factors; D012269:Ribosomal Proteins; C027624:ribosomal protein L11