Evaluation of Matrex cellufine GH 25.
The aim of this work was to test a new matrix for group size exclusion chromatography, Matrex cellufine GH 25, and compare it with Sephadex G25 Superfine and Sephadex G25 fine. Matrex cellufine GH 25 showed a better behaviour at high flow rate (792 cm/h) without back-pressure or packing-down. Sephadex G25 superfine showed a limited flow rate (226 cm/h) with both back-pressure and packing-down and Sephades G25 fine allowed running at 792 cm/h but with packing-down fourfold superior to that of Matrex cellufine GH 25. To have the same number of theoretical plates, it was necessary to increase the height of the bed and therefore the volume of the matrix (43%). With Matrex cellufine GH 25, the sample volume was more limited (9% of column volume) than with Sephadex G25 superfine (17%) but was equivalent to Sephadex fine (9%). To have the same elution time as Sephadex G25 superfine, the flow rate had to be increased by a factor 1.7. As Matrex cellufine GH 25 allowed a high flow rate when the volume of the sample was limited to 6% of column volume, its performance is better than that of Sephadex G25.
Vincent
Patrick
P
Ecole Supérieure de Technologie des Biomolécules de Bordeaux (ESTBB), UMR 5544, Université Victor Segalen Bordeaux 2, 146 rue Léo Saignat, 33076 Bordeaux Cedex, France.
Compoint
Jean-Pierre
JP
Fitton
Valérie
V
Santarelli
Xavier
X
D002850:Chromatography, Gel / Q000295:instrumentation* / Q000379:methods*; D003911:Dextrans / Q000737:chemistry*; D005782:Gels / Q000737:chemistry*; D008422:Materials Testing; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D011312:Pressure; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000302:isolation & purification*; D014462:Ultrafiltration / Q000295:instrumentation* / Q000379:methods*
D003911:Dextrans; D005782:Gels; D012710:Serum Albumin, Bovine; C025614:sephadex