Experimental study on the antitumor effect of mouse B7-1 gene.
Mouse B7-1 cDNA was cloned by RT-PCR from BALB/C mouse splenic cells and inserted into pcDNA3 to construct an eukaryotic expression vector. This constructor was named pCD-mB7-1, in which the B7-1 cDNA was identified to be consistent with the data from other researchers. pCD-mB7-1 plasmid was transfected into B16(F0) cells, and effective expression of mB7-1 in these tumor cells could be detected till the 6th month by RT-PCR and RNA hybridization. Specific cytotoxity assay of lymphocytes was conducted after culturing with tumor cells and the results demonstrated that B16 cells transfected with B7-1 gene were more effective than B16-wt and B16-neo in inducing specific cytotoxity of lymphocytes against B16-wt cells. It is suggested that expression of B7-1 gene in tumor cells could enhance the immunogenicity and induce the effective antitumor immunity.
Qu
S
S
Department of Biochemistry, School of Basic Medical Sciences, Tongji Medical University, Wuhan 430030.
Liu
R
R
Wang
J
J
Wang
Y
Y
D000818:Animals; D018122:B7-1 Antigen / Q000235:genetics* / Q000276:immunology*; D003001:Cloning, Molecular; D003602:Cytotoxicity, Immunologic; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D015316:Genetic Therapy; D005822:Genetic Vectors; D008546:Melanoma, Experimental / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009693:Nucleic Acid Hybridization; D013601:T-Lymphocytes / Q000276:immunology; D014162:Transfection
D018122:B7-1 Antigen; D018076:DNA, Complementary