Requirement of modified residue m1A9 for EF-Tu binding to nematode mitochondrial tRNA lacking the T arm.
Most of nematode mitochondrial (mt) tRNAs lacking the T arm have 1-methyladenosine (m1A) at position 9. To investigate the effect of m1A, we constructed a nematode Ascaris suum mt tRNA(Met) containing only m1A9 as the modified nucleoside by means of molecular surgery. Although the unmodified A. suum mt Met-tRNA(Met) did not bind to nematode mt EF-Tu, the m1A9-containing tRNA bound to the EF-Tu, suggesting that m1A at position 9 is necessary for binding of nematode mt tRNAs lacking the T arm to the EF-Tu, probably because of maintenance of the L-shape-like structure or interaction with the C-terminal amino acid residues of the EF-Tu.
Sakurai
M
M
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo.
Ohtsuki
T
T
Watanabe
Y
Y
Watanabe
K
K
D000241:Adenosine / Q000031:analogs & derivatives* / Q000737:chemistry*; D000818:Animals; D017165:Ascaris suum / Q000235:genetics*; D001665:Binding Sites; D010444:Peptide Elongation Factor Tu / Q000378:metabolism*; D012313:RNA / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D000077278:RNA, Mitochondrial; D012358:RNA, Transfer, Met / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism
D000077278:RNA, Mitochondrial; D012358:RNA, Transfer, Met; C002230:1-methyladenosine; D012313:RNA; D010444:Peptide Elongation Factor Tu; D000241:Adenosine