title: Stereospecific analysis of loxoprofen in plasma by chiral column liquid chromatography with a circular dichroism-based detector.
abstract: The chiral separation of loxoprofen was achieved on a chiral column with UV and circular dichroism (CD) detection. The good resolution of four loxoprofen stereoisomers was obtained. The column used for the chiral separation was Chiralcel OJ column (250 x 4.6 mm) using hexane-2-propanol-trifluoroacetic acid (95:5:0.1), as an eluent. The flow-rate was 1.0 ml/min and the detection was at 225 nm. In addition, CD and UV spectra were obtained by stopped flow scanning. The method allows the determination of the stereoisomers of loxoprofen in human plasma after the administration of therapeutic dose of the racemic drug, thus HPLC with CD detector is useful for the stereospecific determination of loxoprofen products in biological samples.
authors.affiliation: Kyoritsu College of Pharmacy, Shibakoen 1-5-30, Minato-ku, Tokyo 105-8512, Japan. kanazawa-hd@kyoritsu-ph.ac.jp
authors.forename: Hideko
authors.initials: H
authors.lastname: Kanazawa
authors.forename: Akane
authors.initials: A
authors.lastname: Tsubayashi
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Nagata
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Matsushima
authors.forename: Chiharu
authors.initials: C
authors.lastname: Mori
authors.forename: Junko
authors.initials: J
authors.lastname: Kizu
authors.forename: Megumu
authors.initials: M
authors.lastname: Higaki
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis / Q000097:blood*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D006801:Humans; D007202:Indicators and Reagents; D010666:Phenylpropionates / Q000032:analysis / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D013607:Tablets
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D007202:Indicators and Reagents; D010666:Phenylpropionates; D013607:Tablets; C040656:loxoprofen