Adenovirus-mediated gene transfer of Bcl-xL impedes neurite regeneration in vitro.
Mouse retinal explants were transfected with recombinant adenovirus vector carrying the green fluorescent protein (GFP) gene and the rat bcl-x(L) gene (Adeno-Bcl-xL) to determine its ability to protect retinal ganglion cells against apoptotic cell death and to promote retinal ganglion cell neurite regeneration. Adeno-Bcl-xL-incubated retinas had reduced apoptosis compared with controls. However, neurite regeneration in adeno-treated retinas was less than that of vector-free retina. These results suggest that the usefulness of adenovirus vectors for gene therapy for retinal ganglion cells may be limited.
Oshitari
Toshiyuki
T
Department of Ophthalmology, Graduate School of Medicine, Chiba University, Inohana 1-8-1, Chuo-ku, Chiba City 260-8670, Japan. tarii@aol.com
Okada
Seiji
S
Tokuhisa
Takeshi
T
Adachi-Usami
Emiko
E
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D016923:Cell Death; D004274:DNA, Recombinant / Q000235:genetics / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000378:metabolism; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D007211:Indoles / Q000378:metabolism; D008164:Luminescent Proteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009416:Nerve Regeneration / Q000187:drug effects* / Q000502:physiology; D016501:Neurites / Q000187:drug effects* / Q000502:physiology / Q000821:virology; D009474:Neurons; D009924:Organ Culture Techniques; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000494:pharmacology*; D051381:Rats; D012160:Retina / Q000166:cytology* / Q000821:virology; D018800:Thy-1 Antigens / Q000378:metabolism; D014162:Transfection / Q000379:methods / Q000662:veterinary; D051020:bcl-X Protein
C494812:Bcl2l1 protein, mouse; C494813:Bcl2l1 protein, rat; D004274:DNA, Recombinant; D004791:Enzyme Inhibitors; D005456:Fluorescent Dyes; D007211:Indoles; D008164:Luminescent Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D018800:Thy-1 Antigens; D051020:bcl-X Protein; D049452:Green Fluorescent Proteins; C007293:DAPI; D019331:NG-Nitroarginine Methyl Ester