Determination of carbamazepine by chemiluminescence detection using chemically prepared tris(2,2'-bipyridine)ruthenium(III) as oxidant.
A new chemiluminescence method for the determination of carbamazepine (CBZ) has been developed. The method is based on the chemiluminescence produced in the reaction of tris(2,2'-bipyridine)ruthenium(III) and CBZ in an acidic medium. The chemiluminescence intensity was enhanced by organic solvents in the reaction system. Under the optimum experimental conditions, the calibration curve was linear over the range 4.0 x 10(-3)-8.6 x 10(-7) mol/L for CBZ. The detection limit (S/N = 3) was 2.5 x 10(-7) mol/L and the relative standard deviation of six replicate measurements was 2.6% for 4.0 x 10(-4) mol/L of CBZ. The possible reaction mechanism were also discussed. The chemiluminescence method was successfully applied to assay the CBZ contents in pharmaceutical tablets.
Lee
Sang Hak
SH
Department of Chemistry, Kyungpook National University, Taegu 702-701, Korea. shlee@knu.ac.kr
Li
Ming
M
Suh
Jung Kee
JK
D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000737:chemistry; D000927:Anticonvulsants / Q000032:analysis*; D002138:Calibration; D002220:Carbamazepine / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007854:Lead / Q000737:chemistry; D008163:Luminescent Measurements; D009942:Organometallic Compounds / Q000737:chemistry; D016877:Oxidants / Q000737:chemistry; D010087:Oxides / Q000737:chemistry; D012997:Solvents; D013607:Tablets
D000927:Anticonvulsants; D007202:Indicators and Reagents; D009942:Organometallic Compounds; D016877:Oxidants; D010087:Oxides; D012997:Solvents; D013607:Tablets; C058901:tris(2,2'-bipyridine)ruthenium III; D007854:Lead; D002220:Carbamazepine; C047365:lead oxide; D015082:2,2'-Dipyridyl