title: Mechanisms of fluoroquinolone resistance in Pseudomonas aeruginosa isolates from canine infections.
abstract: Chronic otitis externa in dogs is often associated with Pseudomonas aeruginosa infection. Fluoroquinolones are often used for treating such infections. Fluoroquinolone resistance mechanisms were characterized in 10 strains of P. aeruginosa isolated from chronic canine otitis externa. Nine out of ten strains harbored a mutation in the gyrA gene and presented an overexpression of efflux pump(s). There was a good correlation between the lipophilicity of the fluoroquinolone being tested and the effect of the efflux pump inhibitor in the final MIC. Therefore, both mechanisms, mutation in the gyrA gene and increased efflux pump(s), seem to play an important role in the acquisition of fluoroquinolone resistance in veterinary clinical isolates of P. aeruginosa. Levels of resistance to fluoroquinolones suggest that they could not be a good choice for systemic therapy of Pseudomonas otitis.
authors.affiliation: Microbiology, Facultad de Veterinaria, Universidad de Las Palmas de Gran Canaria, P.O. Box 550, 35080 Las Palmas de Gran Canaria, Canary Islands, Spain. mtejedor@dcc.ulpgc.es
authors.forename: María
authors.initials: M
authors.lastname: Teresa Tejedor
authors.forename: Jose Luis
authors.initials: JL
authors.lastname: Martín
authors.forename: Margarita
authors.initials: M
authors.lastname: Navia
authors.forename: Joan
authors.initials: J
authors.lastname: Freixes
authors.forename: Jordi
authors.initials: J
authors.lastname: Vila
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology* / Q000627:therapeutic use; D004269:DNA, Bacterial / Q000032:analysis; D004283:Dog Diseases / Q000188:drug therapy / Q000382:microbiology*; D004285:Dogs; D024881:Drug Resistance, Bacterial; D024841:Fluoroquinolones / Q000494:pharmacology* / Q000627:therapeutic use; D005784:Gene Amplification; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D009154:Mutation; D010032:Otitis Externa / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011552:Pseudomonas Infections / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D000890:Anti-Infective Agents; D004269:DNA, Bacterial; D024841:Fluoroquinolones