Analysis of cDNAs coding for immunologically dominant antigens from an oncosphere-specific cDNA library of Echinococcus multilocularis.
A cDNA library based on mRNA from oncospheres of Echinococcus multilocularis was constructed and screened with an oncosphere-specific rabbit serum. cDNA sequences of three clones that were isolated out of this library are discussed: one codes for a serpin-like proteinase inhibitor, the first isolated from cestodes. Two other clones code for dominant oncosphere antigens and represent homologues of known genes: one is known from several taeniid cestodes as a protective antigen containing fibronectin III domains, the second is related to genes of small heat shock proteins. It contains an internal duplication that might be specific for platyhelminths.
Merckelbach
Armin
A
Department of Parasitology, University of Hohenheim, 70599 Stuttgart, Germany. om6@ix.urz.uni-heidelberg.de
Wager
Martina
M
Lucius
Richard
R
D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D003001:Cloning, Molecular; D004446:Echinococcus / Q000235:genetics* / Q000276:immunology; D015723:Gene Library; D005799:Genes, Dominant / Q000276:immunology; D015151:Immunoblotting; D008969:Molecular Sequence Data
D000947:Antigens, Helminth