[First birth after preimplantation genetic diagnosis performed on thawed embryos].
OBJECTIVE
To report the birth of the first infant conceived after preimplantation genetic diagnosis (PGD) performed on frozen-thawed embryos in our PGD center.


METHODS
Three couples (C1, C2 and C3) who had frozen embryos from a previous in vitro fertilization attempt were enrolled in our PGD program. Embryos were thawed one day before the biopsy procedure for the couples C1 and C3 and the day of the biopsy for the couple C2. The single cell genetic analysis was performed by a multiplex PCR for the couple C1 and by fluorescent in situ hybridization for the couples C2 and C3. The embryos transfers were carried out on the third or fourth day.


RESULTS
Out of ten thawed embryos, eight were biopsied and five were transferred during three embryos transfers. Two biochemical and one ongoing pregnancy were obtained yielded one birth.


CONCLUSIONS
PGD may be offered to couples at risk of transmission of a serious and incurable genetic disease and having frozen embryos.
Frydman
N
N
Service d'Histologie-Embryologie-Cytogénétique à Orientation Biologie et Génétique de la Reproduction, Hôpital Antoine-Béclère (AP-HP), 157, rue de la Porte-de-Trivaux, 92141 Clamart Cedex. nelly.frydman@abc.ap-hop-paris.fr
Ray
P
P
Romana
S
S
Fanchin
R
R
Lelorc'h
M
M
Kerbrat
V
V
Frydman
R
R
Tachdjian
G
G
D001706:Biopsy; D015925:Cryopreservation / Q000379:methods / Q000592:standards; D004624:Embryo Transfer / Q000592:standards; D005260:Female; D005602:France; D030342:Genetic Diseases, Inborn / Q000235:genetics / Q000517:prevention & control; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007231:Infant, Newborn; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011256:Pregnancy Outcome; D018873:Pregnancy Rate; D019836:Preimplantation Diagnosis / Q000379:methods / Q000592:standards; D012307:Risk Factors; D013997:Time Factors