Fecal pyruvate kinase concentrations (ELISA based on a combination of clone 1 and clone 3 antibodies) for gastric cancer screening.
BACKGROUND
Recently we were able to demonstrate that Tumor M2 Pyruvate Kinase (Tumor M2-PK) is detectable in the feces of patients with gastrointestinal (GI) cancer. In this study an ELISA based on a different combination of antibodies was used to investigate stool samples of GI cancer patients.


METHODS
The ELISA (ScheBoBiotech AG, Giessen, Germany) was based on one antibody specific for Tumor M2-PK and a second antibody reacting with Tumor M2-PK, M1-PK and the tetrameric M2-PK. Stool samples of healthy controls, patients with inflammatory bowel disease (IBD) and different kinds of GI cancer were tested.


RESULTS
Compared to controls, samples of IBD or different tumors did not show significant differences, except those patients with gastric cancer (elevated fecal PK in 80% (p = 0.005)).


CONCLUSIONS
If the present results can be confirmed in larger patient samples, this new test might provide an excellent screening tool for gastric cancer.
Hardt
P D
PD
Third Medical Department, Giessen University Hospital, Rodthohl 6, D-35385 Giessen, Germany. philip.d.hardt@innere.med.uni-giessen.de
Toepler
M
M
Ngoumou
B
B
Rupp
J
J
Kloer
H U
HU
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D014408:Biomarkers, Tumor / Q000032:analysis; D015179:Colorectal Neoplasms / Q000175:diagnosis* / Q000201:enzymology; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005243:Feces / Q000201:enzymology*; D005260:Female; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000175:diagnosis; D008297:Male; D008403:Mass Screening / Q000379:methods; D008875:Middle Aged; D011770:Pyruvate Kinase / Q000378:metabolism*; D015203:Reproducibility of Results; D013274:Stomach Neoplasms / Q000175:diagnosis* / Q000201:enzymology
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D011770:Pyruvate Kinase