[Study on molecular mechanism of anti-aging effect of rhidosin on olfactory bulb in senile rats].
OBJECTIVE
To research the molecular mechanism of anti-aging effect of rhidosin on olfactory bulb in senile rats.


METHODS
Ten 3 month-old rats and twenty 26-month-old rats were randomed in rhidosin and saline groups. After decapitated the rats, the olfactory bulb were cut and immediately fixed with neutral formation, followed with paraffin-embedding, serial sectioning, immunohistochemical staining and light microscopic observation.


RESULTS
The proteins of FGF, Bcl-2 and Bax were mainly expressed in mitral cells in the olfactory bulb of the rats. The positive expression rate of FGF and Bcl-2 in young rats group was significantly higher than that in old rats group(P < 0.01). The FGF and Bcl-2 were positive relationship(r = 0.8971; P < 0.01). The expression of Bax in young control group was slightly lower than that in old control group, but there was no significant difference (P > 0.05). The positive expression rate of FGF and Bcl-2 in rhidosin group was significantly higher than that in control group in old rate group(P < 0.01).


CONCLUSIONS
FGF is a factor of ascending the anti-apoptosis gene Bcl-2 in mitral cells; rhidosin can increase the expression of FGF and Bcl-2 proteins. We guess that the mechanism of rhidosin anti-aging effect on the olfactory system may be through the effect of increasing the expression of FGF to inhibit the apoptosis of mitral cells.
Guan
Guimei
G
Department of Otorhinolaryngology-Head and Neck Surgery, China-Japan Union Hospital, Jilin University, Changchun 130031.
Zhu
Dongdong
D
Dong
Zhen
Z
Yang
Zhanquan
Z
D000375:Aging / Q000187:drug effects*; D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005346:Fibroblast Growth Factors / Q000096:biosynthesis*; D019254:Genes, bcl-2 / Q000187:drug effects; D066298:In Vitro Techniques; D009830:Olfactory Bulb / Q000187:drug effects* / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D004365:Drugs, Chinese Herbal; D005346:Fibroblast Growth Factors