Costimulation through the inducible costimulator ligand is essential for both T helper and B cell functions in T cell-dependent B cell responses.
Costimulation through the inducible costimulator (ICOS) and its ligand (ICOSL) is essential for T cell-dependent B cell responses, but the cellular and temporal dynamics underlying its in vivo effects are poorly defined. Here we have shown that Icosl(-/-) and Icos(-/-) mice had similar phenotypes and that ICOS-ICOSL costimulation modulated the early but not late phases of IgG1 affinity maturation. Exploiting the adoptive transfer of T or B cells from primed Icosl(-/-) mice, we provided genetic evidence that costimulation through ICOSL was essential for primary but not secondary helper T cell responses and for the control of both T and B cell activities, resulting in T cell-dependent IgG1 production.
Mak
Tak W
TW
Advanced Medical Discovery Institute, and Ontario Cancer Institute, Department of Medical Biophysics , University of Toronto, Toronto, Ontario M5G 2C1, Canada. tmak@uhnres.utoronto.ca
Shahinian
Arda
A
Yoshinaga
Steve K
SK
Wakeham
Andrew
A
Boucher
Louis-Martin
LM
Pintilie
Melania
M
Duncan
Gordon
G
Gajewska
Beata U
BU
Gronski
Matthew
M
Eriksson
Urs
U
Odermatt
Bernhard
B
Ho
Alexandra
A
Bouchard
Denis
D
Whorisky
John S
JS
Jordana
Manel
M
Ohashi
Pamela S
PS
Pawson
Tony
T
Bladt
Friedhelm
F
Tafuri
Anna
A
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D017353:Gene Deletion; D007074:Immunoglobulin G / Q000276:immunology; D060945:Inducible T-Cell Co-Stimulator Ligand; D060889:Inducible T-Cell Co-Stimulator Protein; D008211:Lymphocyte Cooperation / Q000276:immunology*; D051379:Mice; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
D000945:Antigens, Differentiation, T-Lymphocyte; C556694:Icos protein, mouse; C400230:Icosl protein, mouse; D007074:Immunoglobulin G; D060945:Inducible T-Cell Co-Stimulator Ligand; D060889:Inducible T-Cell Co-Stimulator Protein; D011506:Proteins