Detection of Legionella pneumophila by real-time PCR for the mip gene.
A real-time PCR assay for the mip gene of Legionella pneumophila was tested with 27 isolates of L. pneumophila, 20 isolates of 14 other Legionella species, and 103 non-Legionella bacteria. Eight culture-positive and 40 culture-negative clinical specimens were tested. This assay was 100% sensitive and 100% specific for L. pneumophila.
Wilson
Deborah A
DA
Cleveland Clinic Foundation, Cleveland, Ohio, USA.
Yen-Lieberman
Belinda
B
Reischl
Udo
U
Gordon
Steve M
SM
Procop
Gary W
GW
D001426:Bacterial Proteins / Q000235:genetics; D017931:DNA Primers; D006090:Gram-Negative Bacteria / Q000235:genetics / Q000302:isolation & purification; D006094:Gram-Positive Bacteria / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D020104:Immunophilins / Q000235:genetics*; D007875:Legionella / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D016952:Legionella pneumophila / Q000235:genetics / Q000302:isolation & purification*; D007877:Legionnaires' Disease / Q000382:microbiology; D008565:Membrane Proteins / Q000235:genetics*; D019696:Peptidylprolyl Isomerase; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D001426:Bacterial Proteins; D017931:DNA Primers; D008565:Membrane Proteins; D020104:Immunophilins; C512922:Mip protein, Legionella pneumophila; D019696:Peptidylprolyl Isomerase