Comparative study of tube assembly in three-dimensional collagen matrix and on Matrigel coats.
This study compares phenotypic changes of human umbilical endothelial vein cells cultured in three-dimensional collagen matrixes in the presence of basic fibroblast growth factor or on Matrigel coats. Under both conditions, endothelial cells rapidly assembled into an irregular network of tubular structures with a high frequency of intercellular or lumen-like spaces. Tubular structures were characterized and compared by phase-contrast, confocal and electron microscopy. The dominant mechanism of lumen-like formation was highly model-dependent. Ultrastructural analyses of capillary-like structures and the mechanism of lumen-like formation indicated that the in vivo angiogenesis was better reproduced in the collagen model.
Montañez
Eloi
E
Advancell, Barcelona Scientific Park, Barcelona, Spain.
Casaroli-Marano
Ricardo P
RP
Vilaró
Senén
S
Pagan
Roser
R
D000818:Animals; D002196:Capillaries / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D003094:Collagen; D004338:Drug Combinations; D004730:Endothelium, Vascular / Q000166:cytology* / Q000648:ultrastructure; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D006801:Humans; D007797:Laminin; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D018919:Neovascularization, Physiologic / Q000187:drug effects / Q000502:physiology*; D011509:Proteoglycans; D051381:Rats; D014471:Umbilical Veins
D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; D016222:Fibroblast Growth Factor 2; C059485:matrigel; D003094:Collagen