title: Mapping the distribution of the interstitial telomeric (TTAGGG)n sequences in eight species of Brazilian marsupials (Didelphidae) by FISH and the correlation with constitutive heterochromatin. Do ITS represent evidence for fusion events in American marsupials?
abstract: The C-band pattern and the distribution of the (TTAGGG)(n) sequence after fluorescence in situ hybridization (FISH), were studied in eight species of Didelphidae marsupials: four species with 2n = 14 (Marmosops parvidens, Marmosops incanus, Marmosa murina and Metachirus nudicaudatus), two species with 2n = 18 (Monodelphis domestica and M. americana), and two with 2n = 22 (Didelphis marsupialis and Lutreolina crassicaudata). The hybridization signals were observed at both termini telomeres of all chromosomes. In addition, interstitial sequences were detected in the pericentromeric region of all chromosomes of Marmosops parvidens, in five chromosome pairs of M. incanus, and in the first pair of Monodelphis domestica. These sites always occur in the region of constitutive heterochromatin, even though C-band positive regions do not always present interstitial telomeric sequences (ITS). We suggest that the interstitial (TTAGGG)(n) sequences are associated with satellite DNA and do not necessarily arise through chromosomal rearrangements.
authors.affiliation: Departamento de Biologia, Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: J M
authors.initials: JM
authors.lastname: Pagnozzi
authors.forename: A D
authors.initials: AD
authors.lastname: Ditchfield
authors.forename: Y
authors.initials: Y
authors.lastname: Yonenaga-Yassuda
mesh_terms: D000818:Animals; D001938:Brazil; D002871:Chromosome Banding; D004276:DNA, Satellite / Q000235:genetics; D015321:Gene Rearrangement; D006570:Heterochromatin / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D009893:Opossums / Q000235:genetics*; D010802:Phylogeny; D016615:Telomere / Q000235:genetics*
chemical_list: D004276:DNA, Satellite; D006570:Heterochromatin