Purification, crystallization and preliminary X-ray analysis of a Sco1-like protein from Bacillus subtilis, a copper-binding protein involved in the assembly of cytochrome c oxidase.
The putative copper-delivery protein BsSco from Bacillus subtilis is a member of the Sco family of cytochrome c oxidase assembly proteins. BsSco is a membrane protein and the soluble domain has been cloned and expressed in Escherichia coli as a fusion with glutathione-S-transferase. The fusion protein was isolated from the cell lysate using a glutathione-affinity column and the soluble domain of BsSco was released by treatment with thrombin. Sufficient amounts of the soluble domain have been obtained for crystallization. Crystals obtained by hanging-drop vapour diffusion diffract to a resolution of 2.3 A at a synchrotron source. The space group is P6 and the unit-cell parameters are a = 67.74, b = 67.74, c = 189.58 A.
Imriskova-Sosova
Iveta
I
Department of Biochemistry, Queen's University, Kingston, ON K7L 3N4, Canada.
Ye
Qilu
Q
Hill
Bruce C
BC
Jia
Zongchao
Z
D001412:Bacillus subtilis / Q000737:chemistry*; D001426:Bacterial Proteins; D003001:Cloning, Molecular; D003300:Copper; D003460:Crystallization / Q000379:methods; D003576:Electron Transport Complex IV; D005982:Glutathione Transferase; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins; D012995:Solubility; D013917:Thrombin / Q000378:metabolism; D014961:X-Ray Diffraction / Q000379:methods
D001426:Bacterial Proteins; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; C482909:Sco protein, Bacillus subtilis; D003300:Copper; D003576:Electron Transport Complex IV; D005982:Glutathione Transferase; D013917:Thrombin