title: An efficient chemical method for dephosphorylation of phosphopeptides.
abstract: Phosphate moieties found on serine, threonine or tyrosine residues in peptides, e.g., in proteolytic digests of proteins, were cleaved using hydrofluoric acid or hydrogen fluoride-pyridine without side reactions.
authors.affiliation: Life Science Laboratory, Shimadzu Corporation, Kyoto 604-8511, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kuyama
authors.forename: Chikako
authors.initials: C
authors.lastname: Toda
authors.forename: Makoto
authors.initials: M
authors.lastname: Watanabe
authors.forename: Koichi
authors.initials: K
authors.lastname: Tanaka
authors.forename: Osamu
authors.initials: O
authors.lastname: Nishimura
mesh_terms: D000595:Amino Acid Sequence; D002364:Caseins / Q000378:metabolism; D006858:Hydrofluoric Acid / Q000737:chemistry* / Q000494:pharmacology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D010748:Phosphopeptides / Q000737:chemistry*; D010766:Phosphorylation / Q000187:drug effects; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014357:Trypsin / Q000378:metabolism
chemical_list: D002364:Caseins; D010446:Peptide Fragments; D010748:Phosphopeptides; D014357:Trypsin; D006858:Hydrofluoric Acid