Oligonucleotide probes that detect quantitatively significant groups of butyrate-producing bacteria in human feces.
16S rRNA-targeted oligonucleotide probes were designed for butyrate-producing bacteria from human feces. Three new cluster-specific probes detected bacteria related to Roseburia intestinalis, Faecalibacterium prausnitzii, and Eubacterium hallii at mean populations of 2.3, 3.8, and 0.6%, respectively, in samples from 10 individuals. Additional species-level probes accounted for no more than 1%, with a mean of 7.7%, of the total human fecal microbiota identified as butyrate producers in this study. Bacteria related to E. hallii and the genera Roseburia and Faecalibacterium are therefore among the most abundant known butyrate-producing bacteria in human feces.
Hold
Georgina L
GL
Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, Scotland, UK. g.l.hold@abdn.ac.uk
Schwiertz
Andreas
A
Aminov
Rustam I
RI
Blaut
Michael
M
Flint
Harry J
HJ
D000328:Adult; D002087:Butyrates / Q000378:metabolism*; D005051:Eubacterium / Q000145:classification* / Q000235:genetics; D005243:Feces / Q000382:microbiology*; D005260:Female; D005673:Fusobacterium / Q000145:classification* / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D013045:Species Specificity
D002087:Butyrates; D015345:Oligonucleotide Probes