High-performance liquid chromatographic stationary phases based on poly(methyloctylsiloxane) immobilized on silica. I. Physical and chemical characterizations.
Five different reversed-phase materials for high-performance liquid chromatography were obtained by deposition of poly(methyloctylsiloxane) in HPLC silica particles, followed by immobilization using different processes: thermal treatment (120 or 220 degrees C for 4 h), irradiation with microwaves (495 W for 15 min), gamma radiation (dose of 80 kGy) and self-immobilization. These phases were characterized by gel permeation chromatography, percent carbon, 13C and 29Si solid-state nuclear magnetic resonance spectroscopy, infrared spectroscopy, thermogravimetric analysis and scanning electron microscopy. The results show that the different immobilization processes produce different physical characteristics in the prepared phases.
Tonhi
Edivan
E
Instituto de Química, Universidade Estadual de Campinas, Caixa Postal 6154, 13083-970 Campinas, SP, Brazil.
Bachmann
Stefan
S
Albert
Klaus
K
Jardim
Isabel C S F
IC
Collins
Kenneth E
KE
Collins
Carol H
CH
D002247:Carbon Isotopes; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D007554:Isotopes; D009682:Magnetic Resonance Spectroscopy; D008855:Microscopy, Electron, Scanning; D011108:Polymers / Q000737:chemistry*; D012825:Silicon; D012822:Silicon Dioxide; D012833:Siloxanes / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D013696:Temperature; D013818:Thermogravimetry
D002247:Carbon Isotopes; D007554:Isotopes; D011108:Polymers; D012833:Siloxanes; C475630:poly(methyloctylsiloxane); D012822:Silicon Dioxide; D012825:Silicon