A method for extraction and analysis of high quality genomic DNA from ixodid ticks.
Currently, no published methods describe the extraction of high molecular weight genomic DNA from ixodid ticks (Acari: Ixodidae) and commonly used methods of extraction are not well adapted for use with members of this family. A method for extraction of minimally degraded genomic DNA from ixodid ticks that can be completed in one or two days is described. The method produces DNA which is of sufficient size (>24 kb) for use in Southern analysis and which is readily digestible by restriction endonucleases. Southern analysis using a cytochrome P450 gene probe, demonstrates the success of our method with genomic DNA extracted from two species of Ixodidae, the lone star tick, Amblyomma americanum (Linnaeus) and the cattle fever tick, Boophilus microplus (Canestrini).
Hill
C A
CA
Department of Biological Sciences, University of Notre Dame, PO Box 369, Notre Dame, IN 46556-0369, U.S.A. chill@nd.edu
Gutierrez
J A
JA
D000818:Animals; D015139:Blotting, Southern; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification*; D016678:Genome; D026863:Ixodidae / Q000235:genetics*
D004247:DNA; D003577:Cytochrome P-450 Enzyme System