Regeneration of the mandibular head from grafted periosteum.
Grafted periosteum has a rich potential to induce heterotopic bone formation. In the current study the authors investigate whether autogenous periosteal grafts can regenerate the mandibular head in a rabbit model. They removed the mandibular head of Japanese white rabbits and grafted tibial periosteum to the cut surface of the mandible. Grafted periosteum was observed histologically and radiographically at day 7, 14, 21, and 45 after surgery. At day 7 after grafting, grafted tissue showed remarkable cell proliferation. By 14 days these cells had differentiated into chondrocytes to form cartilage, and endochondral ossification took place after 21 days. At 45 days after surgery, soft X-ray findings showed a newly formed mandibular head, which was similar histologically to that of a normal mandibular head. The cut mandible without periosteal graft showed no regeneration. These findings indicate that grafted periosteum can regenerate the mandibular head without special procedures such as bone fixation in a rabbit model, and suggest that this technique may be useful clinically.
Ueno
Takaaki
T
Departments of Oral and Maxillofacial Reconstructive Surgery, Okayama University Graduate School of Medicine and Dentistry, Japan.
Kagawa
Toshimasa
T
Fukunaga
Jyoji
J
Mizukawa
Nobuyoshi
N
Kanou
Miwa
M
Fujii
Takashi
T
Sugahara
Toshio
T
Yamamoto
Toshio
T
D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000032:analysis; D001861:Bone Regeneration; D024121:Collagen Type X / Q000032:analysis; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000032:analysis; D008334:Mandible / Q000737:chemistry / Q000166:cytology / Q000502:physiology / Q000601:surgery*; D010521:Periosteum / Q000637:transplantation*; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D011817:Rabbits; D000071681:Tartrate-Resistant Acid Phosphatase; D016212:Transforming Growth Factor beta
D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D024121:Collagen Type X; D007527:Isoenzymes; D018809:Proliferating Cell Nuclear Antigen; D016212:Transforming Growth Factor beta; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase