Effect of IL-1 beta and TNF-alpha on the expression of monocyte chemotactic protein-1 in endometriotic cells.
To investigate the clinical significance of monocyte chemotactic protein-1 (MCP-1) produced by endometriotic tissues, the endometriotic tissues were taken from 15 patients with endometriosis. MCP-1 mRNA and MCP-1 protein were determined by dot blot analysis and enzyme linked immunosorbent assay (ELISA) in endometriotic cells cultured with or without interleukin-1 beta (IL-1 beta, 2 micrograms/L), tumor necrosis factor-alpha (TNF-alpha, 20 g/L). After exposure to IL-1 beta or TNF-alpha, the expression of MCP-1 mRNA in the endometriotic cells (8.635 +/- 0.826, 7.031 +/- 0.970, respectively) were significantly higher than that in the control group (4.482 +/- 0.435, P < 0.05); The expression of MCP-1 protein in IL-1 beta and TNF-alpha group was 4.52 +/- 0.09 micrograms/L, 2.87 +/- 0.27 micrograms/L, respectively, which were significantly higher than 1.74 +/- 0.16 micrograms/L in control (P < 0.01). The results suggested that IL-1 beta and TNF-alpha could up-regulate the expression of MCP-1 in endometriotic cells, which might be related to the development of endometriosis.
Gao
Y
Y
Department of Obstetrics and Gynecology, Xiehe Hospital, Tongji Medical University, Wuhan 430022.
Luo
L
L
He
F
F
D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000096:biosynthesis* / Q000235:genetics; D017077:Culture Media, Conditioned; D004715:Endometriosis / Q000378:metabolism* / Q000473:pathology; D004717:Endometrium / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D010532:Peritoneal Diseases / Q000378:metabolism / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D018932:Chemokine CCL2; D017077:Culture Media, Conditioned; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha