Classified fingerprinting: A method of comprehensive analysis for comparing megabase genomes.
The objective of the work we describe is to establish elementary methods for investigating the functions of genes; specifically, a fingerprinting method for analyzing entire DNA fragments in a mixture. Our goal is to develop a method for comparing genes with a size of several megabases. We improved a method of amplified fragment length polymorphism (AFLP) so that it could be used to analyze all the restriction fragments in a mixture. This method could be used to detect 90% of the DNA fragments produced from 100-kb model genomes by using a four-base cutter enzyme.
Okano
K
K
Biosystems Research Department, Central Research Laboratory, Hitachi Ltd.
Uematsu
C
C
Shen
M
M
Kambara
H
H
D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000145:classification; D016172:DNA Fingerprinting / Q000379:methods*; D015894:Genome, Human; D023281:Genomics / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length
D004247:DNA