title: Dual regulation of proton- and sodium-coupled phosphate transport systems in the Yarrowia lipolytica yeast by extracellular phosphate and pH.
abstract: In this study we used a newly isolated osmo-, salt-, and alkali-tolerant Yarrowia lipolytica yeast strain, with a unique capacity to grow over a wide pH range (3.5-10.5). A procedure was elaborated to follow phosphate accumulation by Y. lipolytica cells grown at different pH values. In this paper we demonstrate that Pi-starved Y. lipolytica cells are endowed by derepressible high-affinity, high-capacity H(+)- and Na(+)-driven Pi uptake systems and that activities of these transport systems are under the dual control by the prevailing extracellular Pi concentrations and pH values.
authors.affiliation: A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky pr. 33, Moscow, 119071, Russia. renata_z@inbi.ras.ru
authors.forename: R
authors.initials: R
authors.lastname: Zvyagilskaya
authors.forename: B L
authors.initials: BL
authors.lastname: Persson
mesh_terms: D001692:Biological Transport; D004305:Dose-Response Relationship, Drug; D005110:Extracellular Space / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010710:Phosphates / Q000378:metabolism / Q000493:pharmacokinetics / Q000494:pharmacology; D028081:Proton-Phosphate Symporters / Q000378:metabolism*; D027981:Symporters / Q000378:metabolism*; D025062:Yarrowia / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*
chemical_list: D010710:Phosphates; D028081:Proton-Phosphate Symporters; D027981:Symporters