Oxidative DNA damage estimated by urinary 8-hydroxydeoxyguanosine: influence of taxi driving, smoking and areca chewing.
Nitrogen oxides (NOx) and polycyclic aromatic hydrocarbons are common air pollutants generated from automobile exhaust and cigarette smoke. This study was to investigate urinary 8-hydroxydeoxyguanosine (8-OHdG) as an effective biomarker on DNA damage from traffic exhaust and/or smoking in exposed and non-exposed individuals. With subject consents, the levels of plasma NOx, urinary 1-hydroxypyrene (1-OHP) and 8-OHdG were determined for 95 male taxi drivers and 75 male community residents as the reference group. After adjusting for associate variables, there was a significant correlation between the levels of urinary 8-OHdG and 1-OHP but not NOx. The average level of urinary 8-OHdG was significantly higher in drivers than in community men (13.4+/-4.7 vs. 11.5+/-4.7 microg/g creatinine in mean+/-standard deviation). Compared with non-smoking community men, the multivariate logistic regression showed that the odds ratios (OR) of having elevated levels of urinary 8-OHdG (greater than the overall median value, 12.1 microg/g creatinine) were 6.6 (95% confidence interval (CI)=2.1-20.8) for smoking community men, 5.0 (95% CI=1.7-14.7) for non-smoking taxi drivers and 4.6 (95% CI=1.4-15.0) for smoking taxi drivers. Higher risk was also observed for areca quid chewers compared with non-chewers (OR=1.6; 95% CI=1.1-3.6). In conclusion, taxi driving and smoking may contribute independently to elevated DNA damage using urinary 8-OHdG levels as a sensitive biomarker. This effect is most potent on heavy smokers.
Chuang
Chun-Yu
CY
Institute of Environmental Health, National Taiwan University College of Public Health, No. 1, Jen-Ai Road Sec. 1, Rm 1521, Taipei 100, Taiwan.
Lee
Chen-Chen
CC
Chang
Yu-Kang
YK
Sung
Fung-Chang
FC
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000395:Air Pollutants, Occupational / Q000009:adverse effects*; D001114:Areca / Q000009:adverse effects*; D001334:Automobile Driving; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009589:Nitrogen Oxides / Q000009:adverse effects; D010084:Oxidation-Reduction; D011084:Polycyclic Aromatic Hydrocarbons / Q000009:adverse effects; D012907:Smoking / Q000009:adverse effects*; D001335:Vehicle Emissions / Q000009:adverse effects
D000395:Air Pollutants, Occupational; D009589:Nitrogen Oxides; D011084:Polycyclic Aromatic Hydrocarbons; D001335:Vehicle Emissions; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine