[HPLC-fluorescent spectrometric determination of serum mexiletine concentration after derivatization with fluram].
OBJECTIVE
To establish an HPLC-fluorescent spectrometric method for the determination of mexiletine hydrochloride in plasma after derivatization with fluram.


METHODS
Fluram acetone solution was added to the deproteinized plasma with acetone to obtain the derivative of mexiletine. The HPLC method was performed on a column of Allitima C18 (150 mm x 4.6 mm, 5 microns) with the mobile phase of methanol-water-diethylamine-phosphoric acid buffer (2.4 mol.L-1, pH 4.0) (70:28:2), and the detective wavelength were set at Ex 392 nm and Em 480 nm.


RESULTS
Mexiletine has a liner range over the concentration range from 0.100-6.400 mg.L-1. The lowest detectable concentration of this method was 5 micrograms.L-1 (S/N > or = 4). The intra-day and inter-day RSDs were 1.34%-5.31%, respectively.


CONCLUSIONS
This method is simple, selective and can be used for therapeutic drug monitoring (TDM) and pharmacokinetic studies of mexiletine.
Zhang
Hui
H
Shanghai Xu Hui District Central Hospital, Shanghai 200031, China.
Yu
Chen
C
Liu
Gang-yi
GY
Jia
Jing-ying
JY
Hong
You-cai
YC
Xu
Xiu-rong
XR
D000889:Anti-Arrhythmia Agents / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005450:Fluorescamine / Q000737:chemistry*; D006801:Humans; D008801:Mexiletine / Q000097:blood* / Q000493:pharmacokinetics
D000889:Anti-Arrhythmia Agents; D008801:Mexiletine; D005450:Fluorescamine