Identification of an unknown beta-agonist in feed by liquid chromatography/bioassay/quadrupole time-of-flight tandem mass spectrometry with accurate mass measurement.
A new approach to the search for residues of unknown growth promoting agents such as anabolic steroids and beta-agonists in feed is presented. Following primary extraction and clean-up, samples are separated using gradient liquid chromatography (LC). The effluent is split towards two identical 96-well fraction collectors and an optional electrospray quadrupole time-of-flight mass spectrometry (QTOFMS) system for accurate mass measurement. One 96-well plate is used for a bioassay (enzyme-immuno assay, receptor assay) and will detect the bioactivity and position of the relevant peak in the chromatogram. The positive well in the second 96-well plate is used for identification by LC/QTOFMS/MS. The value of this LC/bioassay/QTOFMS/MS methodology is highlighted by the finding and structure elucidation of a new beta-agonist in a feed extract.
Nielen
M W F
MW
RIKILT Institute of Food Safety, P.O. Box 230, 6700 AE Wageningen, The Netherlands. michel.nielen@wur.nl
Elliott
C T
CT
Boyd
S A
SA
Courtheyn
D
D
Essers
M L
ML
Hooijerink
H H
HH
van Bennekom
E O
EO
Fuchs
R E M
RE
D000318:Adrenergic beta-Agonists / Q000032:analysis*; D000821:Animal Feed / Q000032:analysis*; D002853:Chromatography, Liquid; D007124:Immunoenzyme Techniques; D011869:Radioligand Assay; D021241:Spectrometry, Mass, Electrospray Ionization
D000318:Adrenergic beta-Agonists