Problems connected with band-broadening in size-exclusion chromatography with dual detection.
The sources of band-broadening (axial dispersion) in size-exclusion chromatography (SEC) of polymers and its influence on data obtained using dual detection of concentration and molecular weight are reviewed. Special attention is paid to the combination of a multiangle light-scattering photometer and a differential refractometer as detectors. The local polydispersity is discussed in the relation to the band-broadening as well as to heterogeneity of the polymer with respect to molecular weight and hydrodynamic volume.
Netopilík
Milos
M
Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic. netopil@imc.cas.cz
D002850:Chromatography, Gel / Q000379:methods*; D003198:Computer Simulation; D019544:Equipment Failure Analysis; D008958:Models, Molecular; D008970:Molecular Weight; D010783:Photometry / Q000379:methods*; D012031:Refractometry / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012996:Solutions / Q000737:chemistry*
D012996:Solutions