title: New insight into the haemoglobin superfamily: preliminary crystallographic characterization of human cytoglobin.
abstract: Human cytoglobin, present in almost all tissue types, is a newly identified member of the Hb superfamily. A double mutant, having both cysteines replaced by serines, has been overexpressed in Escherichia coli, purified and crystallized. A highly redundant SAD data set has been collected at the haem Fe-atom absorption edge (lambda = 1.720 A) to 2.60 A resolution. The crystals belong to the orthorhombic P2(1)2(1)2(1) space group, with unit-cell parameters a = 46.8, b = 73.1, c = 98.9 A and two molecules per asymmetric unit. The anomalous difference Patterson map clearly reveals the position of the haem Fe-atom sites, thus paving the way for SAD structure determination.
authors.affiliation: Department of Physics - INFM and Center for Excellence in Biomedical Research, University of Genova, Via Dodecaneso 33, I-16146 Genova, Italy.
authors.forename: Daniele
authors.initials: D
authors.lastname: de Sanctis
authors.forename: Sylvia
authors.initials: S
authors.lastname: Dewilde
authors.forename: Alessandra
authors.initials: A
authors.lastname: Pesce
authors.forename: Paolo
authors.initials: P
authors.lastname: Ascenzi
authors.forename: Thorsten
authors.initials: T
authors.lastname: Burmester
authors.forename: Thomas
authors.initials: T
authors.lastname: Hankeln
authors.forename: Luc
authors.initials: L
authors.lastname: Moens
authors.forename: Martino
authors.initials: M
authors.lastname: Bolognesi
mesh_terms: D001665:Binding Sites; D003460:Crystallization; D018360:Crystallography, X-Ray; D000078842:Cytoglobin; D005914:Globins / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D007501:Iron; D015394:Molecular Structure; D020125:Mutation, Missense
chemical_list: C000627265:CYGB protein, human; D000078842:Cytoglobin; D005914:Globins; D007501:Iron