Effect of denuded endothelial cells on arginine amidase activity released from rabbit arteries.
1. We examined the secretion of arginine amidase activity from rabbit aorta and ear arteries. 2. The amount of arginine amidase activity spontaneously secretion from the aorta was significantly less than that secreted from the ear artery. Dermatan sulphate significantly facilitated the secretion of arginine amidase activity from both the aorta and ear artery. 3. The dermatan sulphate-enhanced secretion of arginine amidase activity from the aorta and ear artery was reduced by denudation of the endothelium. 4. These findings may indicate that spontaneous release of arginine amidase activity from vascular smooth muscle and the enhancement of secretion of arginine amidase activity produced by dermatan sulphate depends on the endothelium.
Naito
Tatsuya
T
Department of Clinical Pharmacology, Meiji Pharmaceutical University and Seikagaku Kogyo Co., Tokyo, Japan.
Ishii-Nozawa
Reiko
R
Miyazaki
Kyosuke
K
Shimokawa
Ken-Ichi
K
Matsuda
Yoshifumi
Y
Takeuchi
Koichi
K
D000818:Animals; D001013:Aorta, Thoracic / Q000201:enzymology / Q000378:metabolism*; D001158:Arteries / Q000201:enzymology / Q000378:metabolism*; D042783:Endothelial Cells / Q000201:enzymology / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000201:enzymology / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D011817:Rabbits; D012697:Serine Endopeptidases / Q000378:metabolism*
D012697:Serine Endopeptidases; C402942:arginine amidase