In vivo and in vitro antioxidant properties of furosemide.
The aim of this study was to investigate in vivo and in vitro antioxidant properties of furosemide. In vitro, human red blood cells were submitted to oxidative stress (AAPH), in absence or in presence of different concentrations of furosemide. Potassium efflux was measured in order to quantify the oxidative stress after the action of AAPH on red blood cells. Allophycocyanin assay was also used to investigate antioxidant capacities of furosemide. For the in vivo experiment, male Wistar rats were used. A control group (n = 5) was treated by a daily intraperitoneal injection of saline solution (0.2 ml); 2 other groups (J0 and J+) were treated for 7 days by one daily intraperitoneal injection of furosemide (0.10 mg/kg/day). In the J+group, the injection of furosemide was done one hour before the experiment, while in the J0 group the last injection of furosemide was done on the 6th day and an injection of saline was performed one hour before the experiment. On the day of experiment, a laparotomy was performed under general anesthesia and blood was collected from abdominal aorta. Oxidative stress and antioxidant capacities were evaluated on Wistar rat red blood cells and plasma. In vitro results (oxidative challenge with AAPH) showed that oxidative stress was decreased in presence of furosemide. This was due to a potent free radical scavenging effect of furosemide. In vivo studies confirmed that furosemide had antioxidant properties. These data may be of great relevance in clinical practice, considering the use of large doses of furosemide in patients presenting pathology involving the production of free radicals.
Lahet
J J
JJ
Laboratoire de Physiopathologie et de Pharmacologie Cardiovasculaires Expérimentales, Facultés de Médecine et de Pharmacie, Universite de Bourgogne, IFR No 100, 7 Boulevard Jeanne d'Arc, 21033 Dijon Cedex, France.
Lenfant
F
F
Courderot-Masuyer
C
C
Ecarnot-Laubriet
E
E
Vergely
C
C
Durnet-Archeray
M J
MJ
Freysz
M
M
Rochette
L
L
D000578:Amidines / Q000737:chemistry; D000818:Animals; D000975:Antioxidants; D004232:Diuretics / Q000737:chemistry / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects; D016166:Free Radical Scavengers / Q000494:pharmacology; D005665:Furosemide / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D010798:Phycocyanin; D011188:Potassium / Q000097:blood; D051381:Rats
D000578:Amidines; D000975:Antioxidants; D004232:Diuretics; D016166:Free Radical Scavengers; C002497:allophycocyanin; D010798:Phycocyanin; C046728:2,2'-azobis(2-amidinopropane); D005665:Furosemide; D011188:Potassium