title: Multiparameter analysis of human oocytes at metaphase II stage after IVF failure in non-male infertility.
abstract: BACKGROUND
  Using fluorescence imaging, an a posteriori multiparametric analysis was performed of human oocytes which failed to give pronucleated zygotes after IVF in cases of very low rates of fertilization or complete fertilization failure.
  
  
  METHODS
  The analysis included: (i) the state of the maternal and paternal chromatin; (ii) quality of the metaphase II oocytes; and (iii) cortical granule (CG) distribution.
  
  
  RESULTS
  Most oocytes were arrested in metaphase II, but they were abnormal in 50% of cases. The incidence of spindle and chromosome aberrations was strongly influenced by maternal age (69% for 40- to 45-year-old women versus 35% for 26- to 33-year-olds), and sperm chromatin was always condensed in immature oocytes, and fully decondensed only in normal metaphase II. The migration of CGs appeared to be associated with achievement of nuclear maturation at the time of puncture.
  
  
  CONCLUSIONS
  These factors, when analysed on a complete set of oocytes from the same patient, provided information about potential causes of IVF failure, and also represented part of an 'oocyte quality evaluation' to select the assisted fertilization technique most suitable for each patient. For example, when the majority of oocytes were judged non-fertilizable at a first attempt, no pregnancy was registered at any subsequent attempt.
authors.affiliation: UMR1198 Biologie du Développement et Reproduction, INRA, Jouy-en-Josas Cedex, France.
authors.forename: Faïçal
authors.initials: F
authors.lastname: Miyara
authors.forename: François-Xavier
authors.initials: FX
authors.lastname: Aubriot
authors.forename: Amélie
authors.initials: A
authors.lastname: Glissant
authors.forename: Catherine
authors.initials: C
authors.lastname: Nathan
authors.forename: Stéphane
authors.initials: S
authors.lastname: Douard
authors.forename: Alexandre
authors.initials: A
authors.lastname: Stanovici
authors.forename: Florence
authors.initials: F
authors.lastname: Herve
authors.forename: Martine
authors.initials: M
authors.lastname: Dumont-Hassan
authors.forename: Alain
authors.initials: A
authors.lastname: LeMeur
authors.forename: Paul
authors.initials: P
authors.lastname: Cohen-Bacrie
authors.forename: Pascale
authors.initials: P
authors.lastname: Debey
mesh_terms: D017677:Age Distribution; D002843:Chromatin / Q000473:pathology; D002869:Chromosome Aberrations; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D015994:Incidence; D007247:Infertility, Female / Q000453:epidemiology / Q000235:genetics / Q000473:pathology*; D008297:Male; D008677:Metaphase; D008870:Microtubules; D009865:Oocytes / Q000473:pathology*; D011247:Pregnancy; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa; D017211:Treatment Failure
chemical_list: D002843:Chromatin