Transfection of fluorescent probed antisense compounds: L-cysteine derivatives of nucleic acid bases.
Antisense with L-cysteine derivative (CAS) can recognize DNA and forms the complementary duplex with DNA. So the properties of CAS in vitro and in vivo were examined in this study. CAS was resistant to proteinase K and stabilized RNA against RNase HI. Moreover using fluorescent CAS, the localization was observed by fluorescence microscope and confocal microscope. As a result, CASs were accumulated inside the nucleus in NG108-15.
Kitaoka
S
S
Graduate School of Osaka University.
Inaki
Y
Y
Maruoka
H
H
Tohnai
N
N
Hatae
T
T
Tanabe
T
T
D000225:Adenine / Q000031:analogs & derivatives; D000818:Animals; D002233:Carbocysteine / Q000737:chemistry*; D002467:Cell Nucleus / Q000737:chemistry; D019286:Endopeptidase K / Q000378:metabolism; D019793:Fluorescein; D005456:Fluorescent Dyes; D008856:Microscopy, Fluorescence; D009696:Nucleic Acids / Q000737:chemistry; D020319:Oligodeoxyribonucleotides, Antisense / Q000032:analysis / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010455:Peptides / Q000737:chemistry; D016914:Ribonuclease H / Q000378:metabolism; D013941:Thymine / Q000031:analogs & derivatives; D014162:Transfection; D014407:Tumor Cells, Cultured
D005456:Fluorescent Dyes; D009696:Nucleic Acids; D020319:Oligodeoxyribonucleotides, Antisense; D010455:Peptides; D002233:Carbocysteine; D016914:Ribonuclease H; C078856:ribonuclease HI; D019286:Endopeptidase K; D000225:Adenine; D013941:Thymine; D019793:Fluorescein