title: Neutrophil-derived hydroxyl radicals mediate interleukin-8-induced increases in tetragastrin-stimulated acid secretion in rats.
abstract: Until recently, the secretion of gastric acid, which plays an important role in the pathogenesis of peptic ulcer, was thought to be controlled by diet, the autonomic nerves and gut hormones. However, peptic ulcer is now known to be caused by the infection with Helicobacter pylori (H. pylori), so it is possible that inflammation modifies the secretion of gastric acid. We used gastric-lumen-perfused rats to first examine the effect of interleukin-8 (IL-8) on acid secretion and then the involvement of free radicals and neutrophils in the action of IL-8. IL-8 enhanced tetragastrin-stimulated acid secretion and free radical scavengers or inhibitors and the pretreatment with anti-rat neutrophil serum inhibited this effect, which indicates that IL-8 enhances gastrin-stimulated acid secretion and that neutrophil-derived hydroxyl radicals mediate the IL-8-induced increase in acid secretion.
authors.affiliation: Third Department of Internal Medicine, Teikyo University School of Medicine, 3426-3 Anesaki, Ichihara-city, Chiba, Japan 299-0111.
authors.forename: Koji
authors.initials: K
authors.lastname: Yakabi
authors.forename: Harumi
authors.initials: H
authors.lastname: Mimura
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Iwabuchi
authors.forename: Shoki
authors.initials: S
authors.lastname: Ro
authors.forename: Hideo
authors.initials: H
authors.lastname: Kamiichi
authors.forename: Seiichi
authors.initials: S
authors.lastname: Miura
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakamura
mesh_terms: D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005744:Gastric Acid / Q000378:metabolism*; D017665:Hydroxyl Radical / Q000494:pharmacology*; D016209:Interleukin-8 / Q000494:pharmacology*; D008297:Male; D009504:Neutrophils / Q000378:metabolism*; D016877:Oxidants / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000494:pharmacology; D013758:Tetragastrin / Q000494:pharmacology*
chemical_list: D016209:Interleukin-8; D016877:Oxidants; D013758:Tetragastrin; D017665:Hydroxyl Radical; D000493:Allopurinol; D013482:Superoxide Dismutase; D004121:Dimethyl Sulfoxide