Functional cloning of genes encoding dsRNA binding proteins.
Over a dozen human genes code for proteins that specifically bind to double-stranded RNA. These proteins have been implicated in several important cellular processes such as transcriptional activation, inhibition of translational initiation, RNA editing, mRNA localization, signal transduction, and posttranscriptional gene silencing (PTGS or RNAi). The recent discovery that PTGS or RNAi is a dsRNA-mediated pathway has further added to the study of dsRNA binding proteins. A method that enables the cloning of genes encoding dsRNA binding proteins would greatly facilitate the identification and study of these molecules. Here we describe a method for isolating such genes from an expression library using radiolabeled poly(I):poly(C) as a binding substrate.
Ramanathan
Y
Y
Center for Applied Genomics, Public Health Research Institute, 225 Warren Street, ICPH W420M, Newark, NJ 07103, USA.
Song
Liting
L
Mathews
Michael B
MB
Tolias
Peter P
PP
D003001:Cloning, Molecular / Q000379:methods*; D005821:Genetic Techniques; D006801:Humans; D011070:Poly I-C; D016601:RNA-Binding Proteins / Q000235:genetics*
D016601:RNA-Binding Proteins; D011070:Poly I-C