title: SIR-dependent repression of non-telomeric genes in Saccharomyces cerevisiae?
abstract: The proteins Sir2, Sir3 and Sir4 repress transcription of the silent mating-type loci HML and HMR and of reporter genes inserted at telomeres. Previous microarray analyses suggested that additional non-telomeric genes exist which are repressed by the Sir proteins. In this study, we tested the expression of 12 such genes by Northern analysis and RT-PCR. However, we were unable to verify their SIR-dependent regulation, which suggests that SIR-mediated repression may be restricted to the known repressed regions.
authors.affiliation: Otto-Warburg-Laboratories, Max-Planck-Institut für Molekulare Genetik, D-14195 Berlin, Germany.
authors.forename: Uta
authors.initials: U
authors.lastname: Marchfelder
authors.forename: Katja
authors.initials: K
authors.lastname: Rateitschak
authors.forename: Ann E
authors.initials: AE
authors.lastname: Ehrenhofer-Murray
mesh_terms: D015152:Blotting, Northern; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal / Q000235:genetics*; D049770:Genes, Mating Type, Fungal; D012331:RNA, Fungal / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae / Q000235:genetics*; D016615:Telomere / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D012331:RNA, Fungal; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae