title: Oxidative DNA damage estimated by urinary 8-hydroxydeoxyguanosine: influence of taxi driving, smoking and areca chewing.
abstract: Nitrogen oxides (NOx) and polycyclic aromatic hydrocarbons are common air pollutants generated from automobile exhaust and cigarette smoke. This study was to investigate urinary 8-hydroxydeoxyguanosine (8-OHdG) as an effective biomarker on DNA damage from traffic exhaust and/or smoking in exposed and non-exposed individuals. With subject consents, the levels of plasma NOx, urinary 1-hydroxypyrene (1-OHP) and 8-OHdG were determined for 95 male taxi drivers and 75 male community residents as the reference group. After adjusting for associate variables, there was a significant correlation between the levels of urinary 8-OHdG and 1-OHP but not NOx. The average level of urinary 8-OHdG was significantly higher in drivers than in community men (13.4+/-4.7 vs. 11.5+/-4.7 microg/g creatinine in mean+/-standard deviation). Compared with non-smoking community men, the multivariate logistic regression showed that the odds ratios (OR) of having elevated levels of urinary 8-OHdG (greater than the overall median value, 12.1 microg/g creatinine) were 6.6 (95% confidence interval (CI)=2.1-20.8) for smoking community men, 5.0 (95% CI=1.7-14.7) for non-smoking taxi drivers and 4.6 (95% CI=1.4-15.0) for smoking taxi drivers. Higher risk was also observed for areca quid chewers compared with non-chewers (OR=1.6; 95% CI=1.1-3.6). In conclusion, taxi driving and smoking may contribute independently to elevated DNA damage using urinary 8-OHdG levels as a sensitive biomarker. This effect is most potent on heavy smokers.
authors.affiliation: Institute of Environmental Health, National Taiwan University College of Public Health, No. 1, Jen-Ai Road Sec. 1, Rm 1521, Taipei 100, Taiwan.
authors.forename: Chun-Yu
authors.initials: CY
authors.lastname: Chuang
authors.forename: Chen-Chen
authors.initials: CC
authors.lastname: Lee
authors.forename: Yu-Kang
authors.initials: YK
authors.lastname: Chang
authors.forename: Fung-Chang
authors.initials: FC
authors.lastname: Sung
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000395:Air Pollutants, Occupational / Q000009:adverse effects*; D001114:Areca / Q000009:adverse effects*; D001334:Automobile Driving; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009589:Nitrogen Oxides / Q000009:adverse effects; D010084:Oxidation-Reduction; D011084:Polycyclic Aromatic Hydrocarbons / Q000009:adverse effects; D012907:Smoking / Q000009:adverse effects*; D001335:Vehicle Emissions / Q000009:adverse effects
chemical_list: D000395:Air Pollutants, Occupational; D009589:Nitrogen Oxides; D011084:Polycyclic Aromatic Hydrocarbons; D001335:Vehicle Emissions; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine