title: Host-dependent tumorigenesis of embryonic stem cell transplantation in experimental stroke.
abstract: The therapeutical potential of transplantation of undifferentiated and predifferentiated murine embryonic stem cells for the regeneration of the injured brain was investigated in two rodent stroke models. Undifferentiated embryonic stem cells xenotransplanted into the rat brain at the hemisphere opposite to the ischemic injury migrated along the corpus callosum towards the damaged tissue and differentiated into neurons in the border zone of the lesion. In the homologous mouse brain, the same murine embryonic stem cells did not migrate, but produced highly malignant teratocarcinomas at the site of implantation, independent of whether they were predifferentiated in vitro to neural progenitor cells. The authors demonstrated a hitherto unrecognized inverse outcome after xenotransplantation and homologous transplantation of embryonic stem cells, which raises concerns about safety provisions when the therapeutical potential of human embryonic stem cells is tested in preclinical animal models.
authors.affiliation: Department of Experimental Neurology, Max-Planck Institute for Neurological Research, University of Cologne, Cologne, Germany.
authors.forename: Franciska
authors.initials: F
authors.lastname: Erdö
authors.forename: Christian
authors.initials: C
authors.lastname: Bührle
authors.forename: James
authors.initials: J
authors.lastname: Blunk
authors.forename: Mathias
authors.initials: M
authors.lastname: Hoehn
authors.forename: Ying
authors.initials: Y
authors.lastname: Xia
authors.forename: Bernd
authors.initials: B
authors.lastname: Fleischmann
authors.forename: Melanie
authors.initials: M
authors.lastname: Föcking
authors.forename: Ekkehardt
authors.initials: E
authors.lastname: Küstermann
authors.forename: Eugen
authors.initials: E
authors.lastname: Kolossov
authors.forename: Jürgen
authors.initials: J
authors.lastname: Hescheler
authors.forename: Konstantin-A
authors.initials: KA
authors.lastname: Hossmann
authors.forename: Thorsten
authors.initials: T
authors.lastname: Trapp
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism / Q000473:pathology; D001932:Brain Neoplasms / Q000209:etiology* / Q000473:pathology; D002454:Cell Differentiation; D002465:Cell Movement; D004195:Disease Models, Animal; D006801:Humans; D020244:Infarction, Middle Cerebral Artery; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D033581:Stem Cell Transplantation; D020521:Stroke / Q000473:pathology / Q000628:therapy; D018243:Teratocarcinoma / Q000209:etiology* / Q000473:pathology; D014183:Transplantation, Heterologous; D014184:Transplantation, Homologous