Involvement of the tyrosine phosphorylation on GSH transport in NIH3T3 fibroblasts.
This study demonstrates the involvement of phosphotyrosine phosphatases on the activity and regulation of GSH ATP-dependent transport system that we have previously identified in NIH3T3 fibroblasts. This is shown by the fact that increases of the initial rate of GSH uptake were measured in NIH3T3 overexpressing a synthetic gene coding for a low-Mr-phosphotyrosine protein phosphatase (LMW-PTP), while decreases were obtained in NIH3T3 overexpressing the phosphatase inactive mutant (LMW-C12SPTP), with respect to NIH3T3neo. Moreover, these results have been confirmed by experiments performed in the same cells by vanadate, and H2O2 treatment on both GSH transport and mediated passive transport of glucose. A possible regulation of this transport system by platelet-derived growth factor receptor (PDGFr) with tyrosine kinase activity is also demonstrated. Moreover, these data show a relationship among GSH, PDGFr and phosphotyrosine phosphatase activity, and suggest a role of GSH transport systems on the cell proliferation process.
Iantomasi
Teresa
T
Department of Biochemical Sciences, University of Firenze, viale Morgagni 50, 50134 Firenze, Italy.
Favilli
Fabio
F
Catarzi
Serena
S
Giannoni
Elisa
E
Biagioni
Chiara
C
Vincenzini
Maria T
MT
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D005347:Fibroblasts / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism / Q000493:pharmacokinetics; D005978:Glutathione / Q000187:drug effects / Q000378:metabolism* / Q000493:pharmacokinetics; D006861:Hydrogen Peroxide / Q000494:pharmacology; D051379:Mice; D009154:Mutation; D041681:NIH 3T3 Cells; D016877:Oxidants / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects; D019000:Phosphotyrosine / Q000378:metabolism; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D017479:Receptors, Platelet-Derived Growth Factor / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism*; D014638:Vanadates / Q000494:pharmacology
D016877:Oxidants; D019000:Phosphotyrosine; D014638:Vanadates; D014443:Tyrosine; D006861:Hydrogen Peroxide; D011505:Protein-Tyrosine Kinases; D017479:Receptors, Platelet-Derived Growth Factor; D017027:Protein Tyrosine Phosphatases; D005978:Glutathione; D005947:Glucose