title: Mutagenesis approaches to deduce structure-function relationships in terpene synthases.
abstract: This review highlights mutagenesis studies of terpene synthases, specifically sesquiterpene synthases and oxidosqualene cyclases. Mutagenesis studies of these enzymes have provided mechanistic insights, structure-function relationships for specific enzymatic residues, novel terpene structures and enzymes with novel activities. The literature through 2002 is reviewed and 113 references cited.
authors.affiliation: Department of Chemistry, Rice University, Houston, Texas 77005, USA.
authors.forename: Michael J R
authors.initials: MJ
authors.lastname: Segura
authors.forename: Beth E
authors.initials: BE
authors.lastname: Jackson
authors.forename: Seiichi P T
authors.initials: SP
authors.lastname: Matsuda
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D002384:Catalysis; D016296:Mutagenesis; D010944:Plants / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism; D013329:Structure-Activity Relationship; D013729:Terpenes / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism
chemical_list: D013729:Terpenes; D019883:Alkyl and Aryl Transferases; C108877:terpene synthase