Root exudate of pmi tomato mutant M161 reduces AM fungal proliferation in vitro.
Soluble factors released from roots of the pre-mycorrhizal infection (pmi) myc(-) tomato mutant M161 were analyzed and compared with normal wild-type released factors. Aseptic whole exudates from the M161 mutant retarded the proliferation of Glomus intraradices in vitro. When the whole exudate was further fractionated on a C18 SEPAK cartridge, the 50/70% methanol fraction showed an activity against hyphal tip growth of Gigaspora gigantea and Gl. intraradices. Preliminary characterization of the exudate suggests that the inhibitory moieties are heat labile, bind to PVPP (polyvinyl polypyrrolidone), and are not volatile. This is the first reported instance of the inhibition by a myc(-) plant being ascribed to inhibitory component(s) released in root exudate.
Gadkar
Vijay
V
Department of Agronomy and Natural Resources, ARO, the Volcani Center, PO Box 6, Bet Dagan 50 250, Israel.
David-Schwartz
Rakefet
R
Nagahashi
Gerald
G
Douds
David D
DD
Wininger
Smadar
S
Kapulnik
Yoram
Y
D001426:Bacterial Proteins; D005658:Fungi / Q000254:growth & development*; D006836:Hydro-Lyases / Q000235:genetics*; D025301:Hyphae / Q000254:growth & development; D066298:In Vitro Techniques; D018551:Solanum lycopersicum / Q000378:metabolism / Q000382:microbiology*; D038821:Mycorrhizae / Q000235:genetics*; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism / Q000382:microbiology*; D013172:Spores, Fungal
D001426:Bacterial Proteins; D010940:Plant Proteins; D006836:Hydro-Lyases; C437623:pmi protein, Streptomyces viridochromogenes