Catalytic spectrophotometric determination of ascorbic acid in tea drink with 1,5-bis(p-hydroxybenzaldene)thiocarbohydrazone as the substrate for horseradish peroxidase.
1,5-bis(p-Hydroxybenzaldene)thiocarbohydrazone (BHBTZ) had been synthesized and used as a new kind of substrate for horseradish peroxidase (HRP). The experiment results showed that ascorbic acid (AsA) had strongly inhibiting effect on the BHBTZ-H(2)O(2)-HRP reaction system, so AsA had been spectrophotomericly determined as the inhibitor by enzymatic inhibition method. Under experimental conditions, the linear relationship between DeltaA(386) and AsA concentration was in the range of 0.82-18.0 microg ml(-1) with a detection limit of 0.245 microg ml(-1). The effect of interferences on AsA determination was investigated. The proposed method was successfully applied to the determination of AsA in tea drink.
Tang
Bo
B
Department of Chemistry, Shandong Normal University, Jinan, 250014, People's Republic of China. tangb@sdnu.edu.cn
Wang
Yan
Y
Du
Ming
M
Ge
Jie-Chao
JC
Chen
Zhen-Zhen
ZZ
D001205:Ascorbic Acid / Q000032:analysis*; D002384:Catalysis; D006735:Horseradish Peroxidase / Q000378:metabolism*; D006835:Hydrazones; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods*; D013662:Tea / Q000737:chemistry*
C477017:1,5-bis(p-Hydroxybenzaldene)thiocarbohydrazone; D006835:Hydrazones; D007202:Indicators and Reagents; D013662:Tea; D006735:Horseradish Peroxidase; D001205:Ascorbic Acid