Effects of L-tetrahydropalmatine on neuron apoptosis during acute cerebral ischemia-reperfusion of rats.
To investigate the effects of L-Tetrahydropalmatine (L-THP) on neuron apoptosis during acute cerebral ischemia-reperfusion of rats and explore the effects of heat shock protein (HSP) on neuron apoptosis, Wistar rats were randomly divided into 3 groups: normal group, ischemia-reperfusion group and treatment group. The condition of neuron apoptosis, the survival state of neuron, pathological changes under an electron microscope and the number of HSP70 positive cells were measured in all groups. Results showed that the apoptosis neuron number was increased obviously at the 24th h during reperfusion and was further increased at the 48th h, the 72th h. While the number of survival neurons was decreased gradually with the prolongation of reperfusion time. Treatment with L-THP could decrease the apoptosis neuron number but increase the survival neuron number and the HSP70 positive cell number. Our study suggested that L-THP could decrease apoptosis and necrosis of neuron, up-regulate the expression of HSP70 and protect the cerebral ischemic injury.
Yang
G
G
Department of Emergency, Tongji Hospital, Tongji Medical University, Wuhan 430030.
Jiang
C
C
Tang
Y
Y
Wang
P
P
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001600:Berberine Alkaloids / Q000494:pharmacology*; D001921:Brain / Q000378:metabolism; D002545:Brain Ischemia / Q000378:metabolism / Q000473:pathology*; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D008297:Male; D009474:Neurons / Q000473:pathology; D009569:Nitric Oxide / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology*
D001600:Berberine Alkaloids; D018840:HSP70 Heat-Shock Proteins; D009569:Nitric Oxide; C014215:tetrahydropalmatine