title: Cullin-5 is ubiquitous in the rat brain.
abstract: Cullin-5 (Cul-5), an E3 ubiquitin ligase that covalently binds ubiquitin to proteins targeted for degradation via the proteasome, was examined for its localization and distribution in the rat central nervous system (CNS). We showed cul-5 mRNA expression in rat neuronal, glial, and vascular endothelial cells by reverse transcription-polymerase chain reaction and corroborated these data by Cul-5 immunostaining in neurons, astrocytes, blood vessels, and choroid plexus of the laboratory rat. Widespread and ubiquitous expression of Cul-5 in the brain suggests that it may have a vital role(s) in cellular activities of the CNS.
authors.affiliation: Graduate Program in Neuroscience, F. Edmund Hébert School of Medicine, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA. tceremuga@usuhs.mil
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Ceremuga
authors.forename: Xiang-Lan
authors.initials: XL
authors.lastname: Yao
authors.forename: Joseph T
authors.initials: JT
authors.lastname: McCabe
mesh_terms: D000818:Animals; D002490:Central Nervous System / Q000033:anatomy & histology / Q000378:metabolism*; D044842:Cullin Proteins; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D020318:Rats, Long-Evans; D017483:Receptors, Vasopressin / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D025801:Ubiquitin / Q000378:metabolism*
chemical_list: C094547:Cul5 protein, rat; D044842:Cullin Proteins; D005904:Glial Fibrillary Acidic Protein; D008565:Membrane Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D017483:Receptors, Vasopressin; D025801:Ubiquitin