Virus-induced silencing of WIPK and SIPK genes reduces resistance to a bacterial pathogen, but has no effect on the INF1-induced hypersensitive response (HR) in Nicotiana benthamiana.
Activation of two mitogen-activated protein kinases (MAPKs), wound-induced protein kinase (WIPK) and salicylic acid-induced protein kinase (SIPK), is one of the earliest responses that occur in tobacco plants that have been wounded, treated with pathogen-derived elicitors or challenged with avirulent pathogens. We isolated cDNAs for these MAPKs (NbWIPKand NbSIPK) from Nicotiana benthamiana. The function of NbWIPK and NbSIPK in mediating the hypersensitive response (HR) triggered by infiltration with INF1 protein (the major elicitin secreted by Phytophthora infestans), and the defense response to an incompatible bacterial pathogen (Pseudomonas cichorii), was investigated by employing virus-induced gene silencing (VIGS) to inhibit expression of the WIPK and SIPK genes in N. benthamiana. Silencing of WIPK or SIPK, or both genes simultaneously, resulted in reduced resistance to P. cichorii, but no change was observed in the timing or extent of HR development after treatment with INF1.
Sharma
P C
PC
Department of Agricultural Botany, Ch. Charan Singh University, 250004 Meerut, India.
Ito
A
A
Shimizu
T
T
Terauchi
R
R
Kamoun
S
S
Saitoh
H
H
D020418:Algal Proteins; D000595:Amino Acid Sequence; D005656:Fungal Proteins / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D007113:Immunity, Innate / Q000235:genetics; D020935:MAP Kinase Signaling System; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics*; D008969:Molecular Sequence Data; D010838:Phytophthora; D010935:Plant Diseases / Q000235:genetics*; D018515:Plant Leaves; D010940:Plant Proteins; D030821:Plants, Genetically Modified; D011506:Proteins; D016415:Sequence Alignment; D014026:Nicotiana / Q000235:genetics*; D014027:Tobacco Mosaic Virus / Q000472:pathogenicity
D020418:Algal Proteins; D005656:Fungal Proteins; D010940:Plant Proteins; D011506:Proteins; C085101:elicitin, Phytophthora; D020928:Mitogen-Activated Protein Kinases; C106405:SA-induced protein kinase; C113423:WIPK protein, Nicotiana tabacum; D020929:Mitogen-Activated Protein Kinase Kinases