Cloning and expression analysis of SALL4, the murine homologue of the gene mutated in Okihiro syndrome.
SALL4 is one out of four human homologues of the DROSOPHILA region-specific homeotic gene SPALT(SAL). Heterozygous mutations of SALL4 on chromosome 20q13.13--> q13.2 cause the autosomal dominant Okihiro syndrome which is characterized by radial limb defects, Duane anomaly and hearing loss. We have partially cloned the murine homologue of this gene, named SALL4, and completed the coding sequence by comparison to available EST and genomic sequences in the GenBank database. This comparison also revealed the chromosomal location of SALL4 on mouse chromosome 2H3 and suggested that a predicted testis expressed gene TEX20 at the very same locus is most likely not a gene on its own but part of the SALL4 3' UTR. We analyzed the expression of SALL4 during early embryogenesis by whole mount in situ hybridization and in the adult mouse by Northern blotting. In adult tissues, SALL4 expression is only found in testis and ovary. During embryonic development, SALL4 expression is widespread in early embryos and becomes gradually confined to the head region and the primitive streak. Prominent expression in the developing midbrain, branchial arches and the limbs suggests an important function of SALL4 during development of these structures as expected from the observation in Okihiro syndrome patients.
Kohlhase
J
J
Institut für Humangenetik, Georg-August-Universität Göttingen, Heinrich-Düker-Weg 12, 37073 Göttingen, Germany. jkohlha@gwdg.de
Heinrich
M
M
Liebers
M
M
Fröhlich Archangelo
L
L
Reardon
W
W
Kispert
A
A
D000367:Age Factors; D000818:Animals; D002874:Chromosome Mapping; D033481:Chromosomes, Mammalian; D003001:Cloning, Molecular; D004370:Duane Retraction Syndrome / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D051379:Mice; D009154:Mutation; D014157:Transcription Factors / Q000235:genetics*
C472575:SALL4 protein, human; D014157:Transcription Factors