title: A high fat diet inhibits delta-aminolevulinate dehydratase and increases lipid peroxidation in mice (Mus musculus).
abstract: The aim of this study was examine the effects of high starch (HS) vs. high fat (HF) feeding on blood glycated hemoglobin (GHbA(1c)) level, thiobarbituric acid-reactive species (TBA-RS) concentration and delta-aminolevulinate dehydratase (delta-ALA-D) activity in mice. The GHbA(1c) level was significantly higher in mice fed the HF diet compared with those fed the HS diet. Hepatic, renal, and cerebral TBA-RS concentrations in mice fed the HF diet were significantly greater than in mice fed the HS diet. In addition, positive correlations were found between the GHbA(1c) and TBA-RS levels for hepatic (P < 0.05; r = 0.46), renal (P < 0.003; r = 0.65), and cerebral (P < 0.001; r = 0.69) tissues. The delta-ALA-D hepatic, renal and cerebral activities of mice fed the HF diet were significantly lower than those of mice fed the HS diet. Furthermore, a negative correlation was found between the GHbA(1c) level and delta-ALA-D activity in hepatic (P < 0.001; r = -0.77), renal (P < 0.007; r = -0.60), and cerebral (P < 0.007; r = -0.60) tissues. The results of this study indicate that consumption of a high fat diet promotes oxidative stress related to hyperglycemia, which in turn can stimulate glycation of proteins leading to delta-ALA-D inhibition in mice.
authors.affiliation: Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, 97105-900, Santa Maria, RS, Brasil.
authors.forename: Vanderlei
authors.initials: V
authors.lastname: Folmer
authors.forename: Júlio C M
authors.initials: JC
authors.lastname: Soares
authors.forename: Diogo
authors.initials: D
authors.lastname: Gabriel
authors.forename: João B T
authors.initials: JB
authors.lastname: Rocha
mesh_terms: D000818:Animals; D001835:Body Weight; D004041:Dietary Fats / Q000008:administration & dosage*; D005260:Female; D006442:Glycated Hemoglobin / Q000378:metabolism; D015227:Lipid Peroxidation; D051379:Mice; D000623:Porphobilinogen Synthase / Q000037:antagonists & inhibitors*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D015430:Weight Gain
chemical_list: D004041:Dietary Fats; D006442:Glycated Hemoglobin; D000623:Porphobilinogen Synthase; D017392:Thiobarbituric Acid Reactive Substances