title: A novel pseudoknot element is essential for the action of a yeast telomerase.
abstract: Telomerase contains an essential RNA, which includes the template sequence copied by the reverse transcription action of telomerase into telomeric DNA. Using phylogenetic comparison, we identified seven conserved sequences in telomerase RNAs from Kluyveromyces budding yeasts. We show that two of these sequences, CS3 and CS4, are essential for normal telomerase function and can base-pair to form a putative long-range pseudoknot. Disrupting this base-pairing was deleterious to cell growth, telomere maintenance, and telomerase activity. Restoration of the base-pairing potential alleviated these phenotypes. Mutating this pseudoknot caused a novel mode of shifting of the boundaries of the RNA template sequence copied by telomerase. A phylogenetically derived model of yeast TER structure indicates that these RNAs can form two alternative predicted core conformations of similar stability: one brings the CS3/CS4 pseudoknot spatially close to the template; in the other, CS3 and CS4 move apart and the conformation of the template is altered. We propose that such disruption of the pseudoknot, and potentially the predicted telomerase RNA conformation, affects polymerization to cause the observed shifts in template usage.
authors.affiliation: Department of Biochemistry and Biophysics, University of California-San Francisco, San Francisco, CA 94143-2200, USA.
authors.forename: Yehuda
authors.initials: Y
authors.lastname: Tzfati
authors.forename: Zachary
authors.initials: Z
authors.lastname: Knight
authors.forename: Jagoree
authors.initials: J
authors.lastname: Roy
authors.forename: Elizabeth H
authors.initials: EH
authors.lastname: Blackburn
mesh_terms: D020029:Base Pairing; D001483:Base Sequence; D017124:Conserved Sequence; D007716:Kluyveromyces / Q000201:enzymology*; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D017423:Sequence Analysis, RNA; D019098:Telomerase / Q000737:chemistry* / Q000378:metabolism
chemical_list: D019098:Telomerase