Edible mushroom (Agaricus bisporus) lectin inhibits human retinal pigment epithelial cell proliferation in vitro.
The retinal pigment epithelium (RPE) plays a major role in the development of the anomalous retinal scarring response termed proliferative vitreoretinopathy. The present study was undertaken to investigate whether agaricus bisporus lectin inhibited human RPE proliferation in vitro. Fluorescein isothiocyanate-labeled agaricus bisporus lectin was used to study binding of lectin to cultured human RPE. The effect of a 24-hour exposure of agaricus bisporus lectin on RPE proliferation was measured using (methyl-3H)-thymidine incorporation into DNA. Toxicity studies were assessed using morphologic evaluation, trypan blue exclusion, and a cell viability assay. Agaricus bisporus lectin bound to RPE cells and was inhibited by preincubation of lectin with asialomucin. Agaricus bisporus lectin caused a dose-dependent inhibition of RPE proliferation (one-way ANOVA, F = 94.470, p < 0.001) that was partially reversible on removal of the lectin. Compared with controls, cells remained viable and no morphological changes or trypan blue staining was noted in RPE exposed to agaricus bisporus lectin. Human RPE binds agaricus bisporus lectin and inhibits proliferation without apparent cytotoxicity. It therefore merits consideration as a potential antiproliferative agent in the prevention and treatment of proliferative vitreoretinopathy and other nonocular anomalous wound healing processes.
Kent
David
D
Unit of Ophthalmology, Department of Medicine, University of Liverpool, United Kingdom. dkent@liv.ac.uk
Sheridan
Carl M
CM
Tomkinson
Heather A
HA
White
Sarah J
SJ
Hiscott
Paul
P
Yu
Lugang
L
Grierson
Ian
I
D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D037102:Lectins / Q000494:pharmacology* / Q000627:therapeutic use; D010857:Pigment Epithelium of Eye / Q000166:cytology* / Q000187:drug effects*; D012163:Retinal Detachment / Q000150:complications / Q000601:surgery; D013997:Time Factors; D018630:Vitreoretinopathy, Proliferative / Q000209:etiology / Q000517:prevention & control
C041433:Agaricus lectins; D037102:Lectins