Preparation of antiserum against rat delta6-desaturase and its use to evaluate the desaturase protein levels in rats treated with gemfibrozil, a ligand for peroxisome proliferator-activated receptor alpha.
Anti-rat delta6-desaturase serum was produced by immunizing rabbits with the 14 N-terminal amino acids (2-15) of rat delta6-desaturase. The antiserum prevented the enzymatic activity of delta6-desaturase in microsomes. Subsequently, the antiserum was used to demonstrate that gemfibrozil, a ligand for peroxisome proliferator-activated receptor alpha, is involved in activating delta6-desaturase gene expression, thereby elevating the protein level and the activity.
Shoji
Yoko
Y
Laboratory of Nutrition Chemistry, Faculty of Bioresources and Bioenvironmental Sciences, Kyushu University, Fukuoka 812-8581, Japan.
Sanekata
Ayako
A
Sato
Masao
M
Imaizumi
Katsumi
K
D000818:Animals; D000906:Antibodies / Q000737:chemistry* / Q000302:isolation & purification; D015248:Gemfibrozil / Q000494:pharmacology*; D000960:Hypolipidemic Agents / Q000494:pharmacology*; D008024:Ligands; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D008970:Molecular Weight; D011817:Rabbits / Q000276:immunology; D051381:Rats; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D013230:Stearoyl-CoA Desaturase / Q000276:immunology*; D014157:Transcription Factors / Q000378:metabolism*
D000906:Antibodies; D000960:Hypolipidemic Agents; D008024:Ligands; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors; D013230:Stearoyl-CoA Desaturase; C474452:delta 6-desaturase, rat; D015248:Gemfibrozil