Biodegradation of polyphenols with immobilized Candida tropicalis under metabolic induction.
During olive oil production, large quantities of olive mill wastewater (OMW) are produced. This wastewater material, containing a high level of phenolic compounds, poses a serious environmental problem in almost all Mediterranean countries. Candida tropicalis YMEC14 was used as an extremophile strain to design an aerobic biotreatment process to detoxify OMW and reduce its polluting organic load. The process was enhanced by directing yeast metabolism towards biodegradation pathways using hexadecane as co-metabolite and by immobilizing yeast cells in calcium alginate beads. Under immobilization conditions, C. tropicalis YMEC14 grown at 40 degrees C in OMW supplemented with hexadecane resulted in 69.7%, 69.2% and 55.3% reduction of chemical oxygen demand, monophenols and polyphenols, respectively, after a 24-h fermentation cycle.
Ettayebi
Khalil
K
Veterinary Molecular Biology, P.O. Box 173610, Montana State University, Bozeman 59717-3142, USA. kettayebi@montana.edu
Errachidi
Faouzi
F
Jamai
Latifa
L
Tahri-Jouti
Mohamed Ali
MA
Sendide
Khalid
K
Ettayebi
Mohamed
M
D000332:Aerobiosis; D000464:Alginates; D001673:Biodegradation, Environmental; D041022:Candida tropicalis / Q000378:metabolism* / Q000648:ultrastructure; D018914:Cells, Immobilized; D005285:Fermentation; D005419:Flavonoids; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D017744:Medical Waste Disposal / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D000069463:Olive Oil; D010636:Phenols / Q000378:metabolism*; D010938:Plant Oils; D011108:Polymers / Q000378:metabolism*; D059808:Polyphenols
D000464:Alginates; D005419:Flavonoids; D006603:Hexuronic Acids; D017744:Medical Waste Disposal; D000069463:Olive Oil; D010636:Phenols; D010938:Plant Oils; D011108:Polymers; D059808:Polyphenols; D020723:Glucuronic Acid