Application of schizophyllan as a novel gene carrier.
Schizophyllan (SPG) is a kind of beta-1,3-glucan which has glucose side chain through a beta-1,6-glycosil bond (Fig. 1). It was found that SPG could form complex with single stranded polynucleotides. SPG forms triple stranded complex with single stranded RNAs thorough hydrogen bonding and hydrophobic interaction. It was found tht polynucleotide was protected from nuclease attacking to form the complex with SPG, and SPG enhanced antisense efficient.
Sakurai
K
K
PRESTO, Japan Science and Technology Corporation, 2432 Aikawa, Kurume, Fukuoka 839-0861, Japan.
Kimura
T
T
Koumoto
K
K
Mizu
M
M
Kobayashi
R
R
Shinkai
S
S
D016373:DNA, Antisense / Q000235:genetics* / Q000378:metabolism; D004337:Drug Carriers; D005090:Exodeoxyribonucleases / Q000378:metabolism; D015870:Gene Expression; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D046911:Macromolecular Substances; D009841:Oligonucleotides / Q000737:chemistry; D012566:Sizofiran / Q000737:chemistry*
D016373:DNA, Antisense; D004337:Drug Carriers; D008164:Luminescent Proteins; D046911:Macromolecular Substances; D009841:Oligonucleotides; D049452:Green Fluorescent Proteins; D012566:Sizofiran; D005090:Exodeoxyribonucleases; C028450:exodeoxyribonuclease I