Monitoring of white-rot fungus during bioremediation of polychlorinated dioxin-contaminated fly ash.
Bioremediation is a low-cost treatment alternative for the cleanup of polychlorinated-dioxin-contaminated soils and fly ash when pollution spread is wide-ranging. An interesting fungus, Ceriporia sp. MZ-340, with a high ability to degrade dioxin, was isolated from white rotten wood of a broadleaf tree from Kyushu Island in Japan. We have attempted to use the fungus for bioremediation of polychlorinated-dioxin-contaminated soil on site. However, we have to consider that this trial has the potential problem of introducing a biohazard to a natural ecosystem if this organism is naturalized. We have therefore developed a monitoring system for the introduced fungus as a part of the examination and evaluation of bioremediation in our laboratory. We have also developed a PCR-based assay to reliably detect the fungus at the bioremediation site. DNA isolated from the site was amplified by PCR using a specific primer derived from internal transcribed spacer region (ITS: ITS1, 5.8S rDNA and ITS2) sequences of Ceriporia sp. MZ-340. We successfully monitored Ceriporia sp. MZ-340 down to 100 fg/ micro l DNA and down to 2 mg/g mycelium. We also successfully monitored the fungus specifically at the bioremediation site. The polychlorinated dibenzo- p-dioxin and polychlorinated dibenzofuran content was observed to decrease in response to treatment with the fungus. The species-specific PCR technique developed in the present work is useful in evaluating the possibility of on-site bioremediation using the fungus Ceriporia sp. MZ-340.
Suhara
H
H
Laboratory of Systematic Forest and Forest Products Sciences, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581 Fukuoka, Japan.
Daikoku
C
C
Takata
H
H
Suzuki
S
S
Matsufuji
Y
Y
Sakai
K
K
Kondo
R
R
D000393:Air Pollutants / Q000378:metabolism; D001572:Benzofurans / Q000032:analysis; D001673:Biodegradation, Environmental; D002244:Carbon / Q000032:analysis / Q000737:chemistry; D060729:Coal Ash; D004271:DNA, Fungal / Q000032:analysis; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D004147:Dioxins / Q000032:analysis / Q000378:metabolism*; D052638:Particulate Matter; D016133:Polymerase Chain Reaction / Q000379:methods; D020072:Polyporales / Q000302:isolation & purification* / Q000378:metabolism; D012680:Sensitivity and Specificity
D000393:Air Pollutants; D001572:Benzofurans; D060729:Coal Ash; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D004147:Dioxins; D052638:Particulate Matter; D002244:Carbon; C023614:dibenzofuran; C020887:dibenzo(1,4)dioxin