Template directed DNA photoligation via substituted 2'-deoxyuridine.
An efficient and reversible template-directed photoligation of oligodeoxynucleotides (ODNs) using 5-carboxyvinyldeoxyuridine (CVU) was described. We have also demonstrated about photochemical synthesis of circularized DNA and padlocking plasmid DNA via CVU mediated photoreaction.
Fujimoto
K
K
Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Kyoto 606-8501, Japan.
Ogawa
N
N
Matsuda
S
S
Saito
I
I
D001483:Base Sequence; D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry; D004270:DNA, Circular / Q000138:chemical synthesis / Q000737:chemistry; D003857:Deoxyuridine / Q000031:analogs & derivatives*; D024202:Electrophoretic Mobility Shift Assay; D008027:Light; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D010957:Plasmids / Q000138:chemical synthesis; D013698:Templates, Genetic
D004270:DNA, Circular; D009838:Oligodeoxyribonucleotides; D004247:DNA; D003857:Deoxyuridine