title: Phenotypic modulation of the stromal reticular network in normal and neoplastic lymph nodes: tissue transglutaminase reveals coordinate regulation of multiple cell types.
abstract: The lymph node paracortex is composed of a network of fibroblastic reticular cells (FRC) and reticular fibers linking sinuses to blood vessels. Using immunostaining for the inducible enzyme/adhesion molecule tissue transglutaminase (TG), we demonstrate coordinate regulation of multiple stromal cell types of this reticular network including FRC, endothelial cells and sinus lining cells. Tissue transglutaminase is expressed at low levels in the paracortex around primary follicles but is markedly up-regulated in stromal cells around hyperplastic germinal centers and then down-regulated around regressing follicles. In cultured FRC, TG is induced 5- to 20-fold and becomes colocalized with matrix proteins by treatment with interleukin-4, but not by other cytokines that are commonly increased in lymph upon immune activation. Coordinate TG up-regulation is observed surrounding nodules of follicular lymphoma (14 of 15 cases) and in the FRC and endothelium of classical Hodgkin's disease, two tumor types that also showed an activated paracortical phenotype. Small lymphocytic lymphoma showed minimal TG staining, with other lymphoma types showing patterns suggesting differential TG regulation. Using TG as a stromal marker, we have identified differential modulation of the phenotype of the lymph node reticular network that parallels change in the B-cell compartment.
authors.affiliation: Department of Hematopathology, University of Texas M.D. Anderson Cancer Center, and the Department of Pathology, University of Texas, Houston Medical School, Houston, Texas, USA.
authors.forename: Vilmos A
authors.initials: VA
authors.lastname: Thomazy
authors.forename: Francisco
authors.initials: F
authors.lastname: Vega
authors.forename: L Jeffrey
authors.initials: LJ
authors.lastname: Medeiros
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Davies
authors.forename: Dan
authors.initials: D
authors.lastname: Jones
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006801:Humans; D007150:Immunohistochemistry; D015847:Interleukin-4 / Q000378:metabolism; D008198:Lymph Nodes / Q000166:cytology* / Q000201:enzymology / Q000473:pathology; D008223:Lymphoma / Q000201:enzymology* / Q000473:pathology; D010641:Phenotype; D017154:Stromal Cells / Q000166:cytology / Q000201:enzymology*; D014158:Transcription, Genetic; D011503:Transglutaminases / Q000235:genetics / Q000378:metabolism*
chemical_list: D015847:Interleukin-4; D011503:Transglutaminases