Alternate-strand triple-helix formation by the 3'-3'-linked oligodeoxynucleotides with the intercalators at the junction point.
3'-3'-Linked oligodeoxynucleotides (ODNs) with the anthraquinonyl group at the junction point were synthesized on a DNA synthesizer using a controlled pore glass (CPG), which has pentaerythritol carrying the intercalator at one of the four hydroxymethyl groups. Stability of the triplexes with the target duplexes was studied by thermal denaturation. The 3'-3'-linked ODNs with the anthraquinonyl group enhanced the thermal stability of the triplexes when compared with those without the intercalator and the unmodified nonamer. The inhibitory activity of the 3'-3'-linked ODNs against the cleavage of the target DNA by the restriction enzyme Hind III was tested. It was found that the 3'-3'-linked ODN with the anthraquinonyl group at the junction point inhibited the cleavage by the enzyme more effectively than the nonamer and the 3'-3'-linked ODN without the intercalator.
Ueno
Y
Y
Graduate School of Pharmaceutical Sciences, Hokkaido University.
Mikawa
M
M
Hoshika
S
S
Takeba
M
M
Kitade
Y
Y
Matsuda
A
A
D000880:Anthraquinones / Q000737:chemistry*; D001483:Base Sequence; D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D015247:Deoxyribonuclease HindIII / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D007364:Intercalating Agents / Q000737:chemistry*; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D013696:Temperature
D000880:Anthraquinones; D004791:Enzyme Inhibitors; D007364:Intercalating Agents; D009838:Oligodeoxyribonucleotides; C051802:triplex DNA; D004247:DNA; D015247:Deoxyribonuclease HindIII