title: Myocardial viability twenty-four hours after orthotopic heart transplantation from non-heart-beating donors.
abstract: OBJECTIVE
  Using a new preservation strategy, we investigated the performance of hearts from non-heart-beating donors during an observation period of 24 hours after orthotopic heart transplantation in a pig model.
  
  
  METHODS
  In the control group (n = 6) beating donor hearts were harvested with Bretschneider's HTK solution and transplanted orthotopically without reperfusion modifications. In the non-heart-beating donor group (n = 6) hearts were perfused with leukocyte-depleted blood cardioplegia after 30 minutes of normothermic ischemia. Blood cardioplegia was supplemented with a sodium-hydrogen exchange inhibitor and adenosine. After transplantation, a second controlled reperfusion with blood cardioplegia was performed.
  
  
  RESULTS
  Preload recruitable stroke work of the left ventricle 24 hours after transplantation in the control versus non-heart-beating donor group was 108% +/- 24% versus 103% +/- 18% of baseline values. Myocardial blood flow of the left and right ventricle was increased to 146% +/- 32% and 176% +/- 51% in the control group versus 176% +/- 29% and 194% +/- 27% in the non-heart-beating donor group. Myocardial oxygen consumption was 11.2 +/- 2.1 versus 12.8 +/- 2.2 mL/100 g per minute at baseline and 11.6 +/- 2.6 versus 13.2 +/- 3.1 mL/100 g per minute after 24 hours (not significant). Histologic examination with Luxol fast blue staining revealed that 2.6% +/- 4.8% of myocytes in the control group versus 1.8% +/- 1.9% in the non-heart-beating donor group were damaged irreversibly.
  
  
  CONCLUSIONS
  Recovery of donor hearts from non-heart-beating donors is comparable with recovery of organs harvested from heart-beating donors if the above-mentioned preservation technique is used. These results could encourage the use of marginal donor hearts and help to expand the limited donor pool.
authors.affiliation: Department of Cardiovascular Surgery and the Institute of Pathological Anatomy, Albert-Ludwigs-University Medical Center, Freiburg, Germany. Martin@ch11.ukl.uni-freiburg.de
authors.forename: Juergen
authors.initials: J
authors.lastname: Martin
authors.forename: Georg
authors.initials: G
authors.lastname: Lutter
authors.forename: Christian
authors.initials: C
authors.lastname: Ihling
authors.forename: Matthias
authors.initials: M
authors.lastname: Siepe
authors.forename: Susanne
authors.initials: S
authors.lastname: Wagner
authors.forename: Jan
authors.initials: J
authors.lastname: Hilberath
authors.forename: Michael
authors.initials: M
authors.lastname: Kemper
authors.forename: Koppany
authors.initials: K
authors.lastname: Sarai
authors.forename: Friedhelm
authors.initials: F
authors.lastname: Beyersdorf
mesh_terms: D000818:Animals; D003326:Coronary Circulation / Q000502:physiology; D003402:Creatine Kinase / Q000378:metabolism; D052279:Creatine Kinase, MB Form; D005374:Filtration; D006085:Graft Survival; D016027:Heart Transplantation / Q000379:methods* / Q000502:physiology*; D006439:Hemodynamics; D007527:Isoenzymes / Q000378:metabolism; D015425:Myocardial Reperfusion / Q000379:methods; D009926:Organ Preservation / Q000379:methods*; D013552:Swine; D013997:Time Factors
chemical_list: D007527:Isoenzymes; D003402:Creatine Kinase; D052279:Creatine Kinase, MB Form