Inhibition of ICAM-1 gene expression by antisense 2',4'-BNA oligonucleotides.
We have previously reported that 2'-O,4'-C-methylene bridged nucleic acid (2',4'-BNA) had unprecedented binding affinity towards its complementary RNA. We reported herein that the antisense 2',4'-BNA oligonucleotides showed potent inhibitory effect on gene expression of Intercellular Adhesion Molecule-1 (ICAM-1) in living cells. The contribution of RNase H to this antisense effect and adequate stability of 2',4'-BNA oligonucleotides to enzymatic degradation were also demonstrated.
Obika
S
S
Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
Hemamayi
R
R
Masuda
T
T
Sugimoto
T
T
Nakagawa
S
S
Mayumi
T
T
Imanishi
T
T
D001483:Base Sequence; D002478:Cells, Cultured; D004247:DNA / Q000737:chemistry; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D006838:Hydrocarbons; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis / Q000235:genetics*; D008697:Methane / Q000031:analogs & derivatives* / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D016376:Oligonucleotides, Antisense / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D018075:RNA, Complementary / Q000737:chemistry
D006838:Hydrocarbons; D016376:Oligonucleotides, Antisense; D018075:RNA, Complementary; D018799:Intercellular Adhesion Molecule-1; C030011:carbene; D004247:DNA; D008697:Methane