title: Effect of neuronal PC12 cells on the functional properties of intestinal epithelial Caco-2 cells.
abstract: The effect of neuronal cells on the functional properties of intestinal epithelial cells was examined by using an in vitro coculture system. Two cell lines, Caco-2 and PC12, were respectively used as intestinal epithelial and enteric neuronal cell models. Coculture of differentiated Caco-2 cells with PC12 caused a significant decrease in the transepithelial electrical resistance (TER) value of the Caco-2 monolayer. The permeability to lucifer yellow (LY) was also significantly increased, suggesting that the tight junction (TJ) of the Caco-2 monolayers was modulated by coculturing with PC12. To identify the TJ-modulating factor presumably secreted from PC12, the effects of the major neurotransmitters on the TER value and LY transport were examined, but no influence was apparent. The TJ-modulating effect of PC12 was prevented by exposing PC12 to cycloheximide, suggesting that new protein synthesis in PC12 was necessary for this regulation.
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. asatsu@mail.ecc.u-tokyo.ac.jp
authors.forename: Hideo
authors.initials: H
authors.lastname: Satsu
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Yokoyama
authors.forename: Nobumasa
authors.initials: N
authors.lastname: Ogawa
authors.forename: Yoko
authors.initials: Y
authors.lastname: Fujiwara-Hatano
authors.forename: Makoto
authors.initials: M
authors.lastname: Shimizu
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D018920:Coculture Techniques / Q000379:methods; D003513:Cycloheximide / Q000494:pharmacology; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology*; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D018377:Neurotransmitter Agents / Q000494:pharmacology; D016716:PC12 Cells; D019899:Paracrine Communication; D010539:Permeability; D014176:Protein Biosynthesis; D011506:Proteins / Q000502:physiology; D051381:Rats; D019108:Tight Junctions / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D018377:Neurotransmitter Agents; D011506:Proteins; D014409:Tumor Necrosis Factor-alpha; D003513:Cycloheximide