Preparation of monodisperse porous polymethacrylate microspheres and their application in the capillary electrochromatography of macrolide antibiotics.
Monodisperse poly(glycidyl methacrylate-divinylbenzene) microspheres were prepared by a simple one-step dispersion polymerization process. Examination of the polymeric microspheres showed that they had a mean particle diameter of 3 microm and dual pore size distribution with mean pore diameters of 300 and 800 A. The microspheres were functionalized by introducing quaternary ammonium/octadecyl groups to obtain positively charged beads in a wide pH range. The functionalized beads were packed into fused-silica capillary having 50 microm inner diameter and used to separate erythromycin derivatives by capillary electrochromatography (CEC). These samples require gradient elution when separated by high-performance liquid chromatography (HPLC) or micro-HPLC, but with the new columns isocratic elution suffices for their separation by CEC. The column efficiency ranged from 40,000 to 50,000 theoretical plates.
Zhang
Shuhong
S
Department of Chemical Engineering, Yale University, P.O. Box 208286, New Haven, CT 06520-8286, USA.
Huang
Xian
X
Yao
Naisheng
N
Horváth
Csaba
C
D000900:Anti-Bacterial Agents / Q000032:analysis*; D019075:Electrophoresis, Capillary; D004917:Erythromycin / Q000031:analogs & derivatives / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D010316:Particle Size; D011109:Polymethacrylic Acids / Q000737:chemistry*; D016062:Porosity; D013343:Styrenes / Q000737:chemistry
D000900:Anti-Bacterial Agents; D011109:Polymethacrylic Acids; D013343:Styrenes; C076011:1,3-diethenylbenzene; C030613:polymethacrylic acid; D004917:Erythromycin