[Comparison of BBL mycobacteria growth indicator tube (MGIT) method, BACTEC radiometric system and Lowenstein-Jensen culture media for the detection of mycobacteria in clinical specimens].
The need for more rapid diagnostic tests for early detection of mycobacteria in clinical specimens has become more apparent for the control of tuberculosis. Several commercial systems, as well as conventional media, are available for the culture of acid-fast bacteria (AFB). The aim of this study was to evaluate the Mycobacteria Growth Incubator Tube (MGIT), BACTEC 460 and Lowenstein-Jensen (LJ) media for the recovery of mycobacteria from processed clinical specimens. A total of 75 clinical specimens which were found positive for AFB in smear microscopy were included to the study, and of them 73 were grown in MGIT system, 70 in BACTEC 460 system and 74 on the LJ slants. Average periods for the detection of mycobacteria for BACTEC 460, MGIT systems and LJ medium were 7.3 days, 10.9 days and 19.4 days, respectively. As a result, it was found that the recovery of mycobacteria in MGIT system is comparable to BACTEC 460 system. It appears to be a safer system than BACTEC 460 system, and may be used for detection of mycobacteria in laboratories desiring a non-radioactive method.
Alp
Alpaslan
A
Hacettepe Universitesi Tip Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dali, Ankara.
Hasçelik
Gülşen
G
D001431:Bacteriological Techniques / Q000379:methods / Q000592:standards*; D003470:Culture Media; D006801:Humans; D009161:Mycobacterium / Q000254:growth & development / Q000302:isolation & purification*; D009164:Mycobacterium Infections / Q000175:diagnosis* / Q000382:microbiology / Q000517:prevention & control; D013997:Time Factors
D003470:Culture Media