title: Molecular cloning and characterization of novel centromeric repetitive DNA sequences in the blue-breasted quail (Coturnix chinensis, Galliformes).
abstract: A new family of centromeric highly repetitive DNA sequences was isolated from EcoRI-digested genomic DNA of the blue-breasted quail (Coturnix chinensis, Galliformes), and characterized by filter hybridization and chromosome in situ hybridization. The repeated elements were divided into two types by nucleotide length and chromosomal distribution; the 578-bp element predominantly localized to microchromosomes and the 1,524-bp element localized to chromosomes 1 and 2. The 578-bp element represented tandem arrays and did not hybridize to genomic DNAs of other Galliformes species, chicken (Gallus gallus), Japanese quail (Coturnix japonica) and guinea fowl (Numida meleagris). On the other hand, the 1,524-bp element was not organized in tandem arrays, and did hybridize to the genomic DNAs of three other Galliformes species, suggesting that the 1,524-bp element is highly conserved in the Galliformes. The 578-bp element was composed of basic 20-bp internal repeats, and the consensus nucleotide sequence of the internal repeats had homologies to the 41-42 bp CNM repeat and the XHOI family repeat of chicken. Our data suggest that the microchromosome-specific highly repetitive sequences of the blue-breasted quail and chicken were derived from a common ancestral sequence, and that they are one of the major and essential components of chromosomal heterochromatin in Galliformes species.
authors.affiliation: Laboratory of Animal Cytogenetics, Center for Advanced Science and Technology, Hokkaido University, North 10 West 8, Kita-ku, Sapporo 060-0810, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yamada
authors.forename: M
authors.initials: M
authors.lastname: Shibusawa
authors.forename: M
authors.initials: M
authors.lastname: Tsudzuki
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuda
mesh_terms: D000818:Animals; D001483:Base Sequence; D002503:Centromere / Q000235:genetics*; D002642:Chick Embryo; D002875:Chromosomes / Q000235:genetics; D003001:Cloning, Molecular; D016384:Consensus Sequence; D003370:Coturnix / Q000235:genetics*; D004276:DNA, Satellite / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D013045:Species Specificity
chemical_list: D004276:DNA, Satellite