title: Histochemical study of glycoconjugates in active and photoperiodically-regressed testis of hamster (Mesocricetus auratus).
abstract: The objective of the present study was to characterize glycoconjugates of hamster testis in gonadally-active and -inactive states by lectin histochemical methods. Thirteen HRP- or digoxigenin-labeled lectins were used in samples obtained from fertile and photoinhibited hamsters. In gonadally-active hamsters, spermatozoa tails were stained with Con-A, HPA, PNA, UEA-I, LTA, AAA, WGA and LFA and weakly with GNA and RCA-I. Spermatozoa acrosomes were labeled with HPA, SBA, WGA and PNA. Spermatid acrosomes were labeled with SBA, RCA-I, PNA, and WGA. Staining with GNA and Con-A was found in the Golgi phase and HPA staining was found in the Golgi phase and maturated spermatids. Cytoplasm of spermatocytes was labeled with Con-A, GNA, LTA, AAA, RCA-I, HPA, WGA and LFA, whereas spermatocyte membranes were stained with Con-A, LTA and AAA. Spermatogonia were strongly labeled with Con-A and moderately labeled with AAA, WGA and LFA. Sertoli cells were positive after staining with Con-A, AAA, WGA, and LFA. The lamina propria was positive after staining with UEA-I, LTA, AAA and LFA. Leydig cells showed strong labeling with SBA, Con-A, GNA, SNA and MAA, moderate labeling with WGA, weak labeling with RCA-I, AAA and LFA. In gonadally-inactive hamsters, spermatocytes showed increased staining with HPA, PNA and AAA, whereas staining with Con-A, GNA and LTA had disappeared. Spermatogonia showed an increased labeling with AAA and WGA, but labeling with Con-A and LFA had disappeared. Sertoli cells were strongly labeled with GNA. Con-A and GNA staining was decreased in Leydig cells of gonadally-inactive hamsters but PNA and HPA staining was increased. The lamina propria in regressed testes showed intense labeling with PNA. These results suggest that histological, morphological and hormonal changes occurring in hamster testis during exposure to a short photoperiod are reflected in altered patterns of expression and distribution of N- and O-linked glycans.
authors.affiliation: Department of Cellular Biology, Section of Histology and General Embryology, Medical School, University of Murcia, Spain. bioetica@um.es
authors.forename: Luis M
authors.initials: LM
authors.lastname: Pastor
authors.forename: Eva
authors.initials: E
authors.lastname: Morales
authors.forename: Luis A
authors.initials: LA
authors.lastname: Polo
authors.forename: Alfonso
authors.initials: A
authors.lastname: Calvo
authors.forename: Jacinto
authors.initials: J
authors.lastname: Pallarés
authors.forename: Sheila
authors.initials: S
authors.lastname: De La Viesca
mesh_terms: D000818:Animals; D006224:Cricetinae; D006001:Glycoconjugates / Q000032:analysis*; D006651:Histocytochemistry / Q000379:methods; D037102:Lectins / Q000032:analysis; D007985:Leydig Cells / Q000737:chemistry / Q000166:cytology; D008297:Male; D017440:Photoperiod; D011134:Polysaccharides / Q000032:analysis; D013737:Testis / Q000033:anatomy & histology / Q000737:chemistry*
chemical_list: D006001:Glycoconjugates; D037102:Lectins; D011134:Polysaccharides