title: Unique scanning capabilities of a new hybrid linear ion trap mass spectrometer (Q TRAP) used for high sensitivity proteomics applications.
abstract: The unique scanning capabilities of a hybrid linear ion trap (Q TRAP) mass spectrometer are described with an emphasis on proteomics applications. The combination of the very selective triple quadrupole based tandem mass spectrometry (MS/MS) scans with the very sensitive ion trap product ion scans allows rapid identification of peptides at low concentrations derived from post-translationally modified proteins on chromatographic time scales. The Q TRAP instrument also offers the opportunity to conduct a variety of ion processing steps prior to performing a mass scan. For example, the enhancement of the multiple-charge ion contents of the ion trap can be performed resulting in a survey mass spectrum dominated by double- and triple-charge peptides. This facilitates the identification of relevant biological species in both separated and unseparated peptide mixtures for further MS/MS experiments.
authors.affiliation: MDS Sciex, Concord (Ont), Canada.
authors.forename: J C Yves
authors.initials: JC
authors.lastname: Le Blanc
authors.forename: James W
authors.initials: JW
authors.lastname: Hager
authors.forename: A M Patricia
authors.initials: AM
authors.lastname: Ilisiu
authors.forename: Christie
authors.initials: C
authors.lastname: Hunter
authors.forename: Feng
authors.initials: F
authors.lastname: Zhong
authors.forename: Ivan
authors.initials: I
authors.lastname: Chu
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry / Q000295:instrumentation*; D010748:Phosphopeptides / Q000032:analysis*; D020543:Proteome; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000032:analysis
chemical_list: D010748:Phosphopeptides; D020543:Proteome; D012710:Serum Albumin, Bovine