title: Low-stringency single-specific-primer PCR as a tool for detection of mutations in the rpoB gene of rifampin-resistant Mycobacterium tuberculosis.
abstract: By the low-stringency single-specific-primer PCR technique, a highly sensitive and rapid method for diagnosis of rifampin resistance in Mycobacterium tuberculosis was established. Seven rifampin-resistant and five rifampin-susceptible specimens were analyzed. Rifampin resistance was determined by MIC measurement. A complex electrophoretic pattern consisting of many bands was obtained for both susceptible and rifampin-resistant isolates. The same pattern was obtained for all of the susceptible specimens, but differences between resistant and susceptible isolates were found. DNA sequencing showed that a particular mutation produces a specific electrophoretic pattern.
authors.affiliation: Departamento de Farmácia Social, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. wscarv@icb.ufmg.br
authors.forename: Wania S
authors.initials: WS
authors.lastname: Carvalho
authors.forename: Silvana
authors.initials: S
authors.lastname: Spindola de Miranda
authors.forename: Kátia M
authors.initials: KM
authors.lastname: Costa
authors.forename: José G V C
authors.initials: JG
authors.lastname: Araújo
authors.forename: Claudio J
authors.initials: CJ
authors.lastname: Augusto
authors.forename: João B
authors.initials: JB
authors.lastname: Pesquero
authors.forename: Jorge L
authors.initials: JL
authors.lastname: Pesquero
authors.forename: Maria A
authors.initials: MA
authors.lastname: Gomes
mesh_terms: D000904:Antibiotics, Antitubercular / Q000494:pharmacology*; D001483:Base Sequence; D017931:DNA Primers; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000187:drug effects* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012293:Rifampin / Q000494:pharmacology*; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014397:Tuberculosis, Pulmonary / Q000382:microbiology
chemical_list: D000904:Antibiotics, Antitubercular; D017931:DNA Primers; D012321:DNA-Directed RNA Polymerases; D012293:Rifampin