title: Comparison of genotyping Helicobacter pylori directly from biopsy specimens and genotyping from bacterial cultures.
abstract: PCR for vacA and cagA genotypes of Helicobacter pylori using DNA isolated from infected gastric biopsy specimens was approximately equal to genotyping using bacterial DNA from cultures. Inconsistent results were associated with low H. pylori density in biopsies. A higher proportion of mixed infection was found when biopsies were used.
authors.affiliation: Department of Medicine, Veterans Affairs Medical Center, and Baylor College of Medicine, Houston, Texas, USA.
authors.forename: Chang-Young
authors.initials: CY
authors.lastname: Park
authors.forename: Minjung
authors.initials: M
authors.lastname: Kwak
authors.forename: Oscar
authors.initials: O
authors.lastname: Gutierrez
authors.forename: David Y
authors.initials: DY
authors.lastname: Graham
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Yamaoka
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D001706:Biopsy; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis* / Q000302:isolation & purification; D005838:Genotype; D016481:Helicobacter Infections / Q000382:microbiology; D016480:Helicobacter pylori / Q000145:classification* / Q000235:genetics / Q000254:growth & development*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D013270:Stomach / Q000382:microbiology*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D003470:Culture Media; D004269:DNA, Bacterial; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori