title: In situ identification of intracellular bacteria related to Paenibacillus spp. in the mycelium of the ectomycorrhizal fungus Laccaria bicolor S238N.
abstract: Bacterial proliferations have recurrently been observed for the past 15 years in fermentor cultures of the ectomycorrhizal fungus Laccaria bicolor S238N, suggesting the presence of cryptic bacteria in the collection culture of this fungus. In this study, intracellular bacteria were detected by fluorescence in situ hybridization in combination with confocal laser scanning microscopy in several collection subcultures of L. bicolor S238N. They were small (0.5 micro m in diameter), rare, and heterogeneously distributed in the mycelium and were identified as Paenibacillus spp. by using a 16S rRNA-directed oligonucleotide probe initially designed for bacteria isolated from a fermentor culture of L. bicolor S238N.
authors.affiliation: Institut National de la Recherche Agronomique, UMR "Interactions Arbres/Micro-organismes," Centre de Nancy, 54280 Champenoux, France.
authors.forename: J
authors.initials: J
authors.lastname: Bertaux
authors.forename: M
authors.initials: M
authors.lastname: Schmid
authors.forename: N Chemidlin
authors.initials: NC
authors.lastname: Prevost-Boure
authors.forename: J L
authors.initials: JL
authors.lastname: Churin
authors.forename: A
authors.initials: A
authors.lastname: Hartmann
authors.forename: J
authors.initials: J
authors.lastname: Garbaye
authors.forename: P
authors.initials: P
authors.lastname: Frey-Klett
mesh_terms: D000363:Agaricales / Q000166:cytology / Q000254:growth & development*; D001406:Bacillaceae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D003470:Culture Media; D015342:DNA Probes; D005285:Fermentation; D017404:In Situ Hybridization, Fluorescence; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D025282:Mycelium / Q000254:growth & development*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D003470:Culture Media; D015342:DNA Probes; D012336:RNA, Ribosomal, 16S