Cross-reactivity of the PLATELIA CANDIDA antigen detection enzyme immunoassay with fungal antigen extracts.
We studied the specificity of the PLATELIA CANDIDA Ag enzyme immunoassay by using 130 isolates of 63 clinically relevant fungal species. Antigen extracts of seven Candida spp. (Candida albicans, C. dubliniensis, C. famata, C. glabrata, C. guilliermondii, C. lusitaniae, and C. tropicalis) repeatedly yielded positive reactions (>0.5 ng/ml). Geotrichum candidum and Fusarium verticillioides were found to yield borderline-positive reactions (0.25 to 0.50 ng/ml). Antigen preparations from the other 54 fungal species, including yeasts, molds, dermatophytes, and dimorphic fungi, did not cross-react in the assay.
Rimek
Dagmar
D
Department of Medical Microbiology and Hospital Hygiene, University Hospital, D-18057 Rostock, Germany. drimek@tllv.thueringen.de
Singh
Jagpal
J
Kappe
Reinhard
R
D000946:Antigens, Fungal / Q000276:immunology*; D002175:Candida / Q000145:classification / Q000276:immunology*; D002177:Candidiasis / Q000382:microbiology; D003429:Cross Reactions; D005658:Fungi / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D009181:Mycoses / Q000382:microbiology; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results
D000946:Antigens, Fungal; D011933:Reagent Kits, Diagnostic