cGMP-phosphodiesterase activity is up-regulated in response to pressure overload of rat ventricles.
Although expression of natriuretic peptides in cardiac tissues is up-regulated in response to pressure overload, no significant change in cGMP level in hypertrophied ventricles was observed. Activities of two cyclic nucleotide phosphodiesterase (PDE) isoforms, Ca2+/calmodulin-stimulated PDE (PDE1) and cGMP-stimulated PDE (PDE2), were significantly higher in rat left ventricles 14 days after aortic banding. The absence of significant changes in PDE1A and PDE2A mRNA levels indicated that the two PDE activities were post-transcriptionally up-regulated. These results suggested that the increased cGMP-PDE activity in response to pressure overload plays an important role in neutralizing cGMP action in cardiac tissue.
Yanaka
Noriyuki
N
Discovery Research Laboratory, Tanabe Seiyaku Co., Ltd., Yodogawa-ku, Osaka 532-8505, Japan.
Kurosawa
Yukie
Y
Minami
Kouichi
K
Kawai
Eri
E
Omori
Kenji
K
D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001011:Aorta / Q000502:physiology; D001794:Blood Pressure / Q000502:physiology*; D015152:Blotting, Northern; D006332:Cardiomegaly / Q000503:physiopathology; D002851:Chromatography, High Pressure Liquid; D015342:DNA Probes; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D006321:Heart / Q000503:physiopathology*; D006352:Heart Ventricles / Q000503:physiopathology; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000201:enzymology*; D009929:Organ Size / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation / Q000502:physiology*; D016276:Ventricular Function
D015342:DNA Probes; D018076:DNA, Complementary; D007527:Isoenzymes; D012333:RNA, Messenger; D015106:3',5'-Cyclic-GMP Phosphodiesterases