Direct analysis of chromosome methylation.
DNA methylation is a possible candidate for a genomic imprinting marker in mammals. This epigenetic modification of DNA satisfies several essential criteria for the identification of the parental origin of individual alleles and larger portions of the genome: DNA methylation is stably propagated in somatic cells during cell division, it is reversible, it may inactivate the target sequence, and male and female gametes have different methylation patterns (reviewed in ref. 1).
Bourc'his
D
D
INSERM U383, Hôpital Necker-Enfants Malades, Paris Cedex, France.
Viegas-Péquignot
E
E
D000818:Animals; D002478:Cells, Cultured; D002875:Chromosomes / Q000235:genetics*; D019175:DNA Methylation; D005260:Female; D018392:Genomic Imprinting; D006801:Humans; D008297:Male