title: Restricted clonal expression of IL-2 by naive T cells reflects differential dynamic interactions with dendritic cells.
abstract: Limited frequencies of T cells express IL-2 in primary antigenic responses, despite activation marker expression and proliferation by most clonal members. To define the basis for restricted IL-2 expression, a videomicroscopic system and IL-2 reporter transgenic model were used to characterize dendritic cell (DC)-T cell interactions. T cells destined to produce IL-2 required prolonged interactions with DCs, whereas most T cells established only transient interactions with DCs and were activated, but did not express IL-2. Extended conjugation of T cells with DCs was not always sufficient to initiate IL-2 expression. Thus, there is intrinsic variability in clonal T cell populations that restricts IL-2 commitment, and prolonged engagement with mature DCs is necessary, but not sufficient, for IL-2 gene transcription.
authors.affiliation: Department of Pathology, University of Alabama at Birmingham, AL 35294, USA.
authors.forename: Vincent
authors.initials: V
authors.lastname: Hurez
authors.forename: Arman
authors.initials: A
authors.lastname: Saparov
authors.forename: Albert
authors.initials: A
authors.lastname: Tousson
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Fuller
authors.forename: Takekazu
authors.initials: T
authors.lastname: Kubo
authors.forename: James
authors.initials: J
authors.lastname: Oliver
authors.forename: Benjamin T
authors.initials: BT
authors.lastname: Weaver
authors.forename: Casey T
authors.initials: CT
authors.lastname: Weaver
mesh_terms: D000818:Animals; D002450:Cell Communication; D003713:Dendritic Cells / Q000502:physiology*; D049452:Green Fluorescent Proteins; D007376:Interleukin-2 / Q000235:genetics*; D008164:Luminescent Proteins / Q000235:genetics; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D011948:Receptors, Antigen, T-Cell / Q000502:physiology; D013601:T-Lymphocytes / Q000502:physiology*; D014158:Transcription, Genetic
chemical_list: D007376:Interleukin-2; D008164:Luminescent Proteins; D011948:Receptors, Antigen, T-Cell; D049452:Green Fluorescent Proteins