A variable region on the chlorovirus CVK2 genome contains genes possibly involved in the host range determination.
A 22.6-kbp variable region near the left end of the chlorovirus CVK2 genome which was characterized. This region contained a tandem array of 5 gene copies for Vp260-like protein, a viral surface glycoprotein. The authentic 104-kDa Vp260 was encoded at another site on the genome and contained 13 internally located, tandem repeats of 61-65 amino acids like the prominent Rickettsia surface antigen. By Northern and Western blot analyses, these genes were demonstrated to be expressed late in infection and the proteins were incorporated into virions. These results implied that the extra copies of Vp260-like proteins may be involved in host range in the natural environment.
Chuchird
N
N
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-4-1 Kagamiyama, Higashi-Hiroshima 739-8527, Japan.
Nishida
K
K
Kawasaki
T
T
Yamada
T
T
D018628:Gene Dosage; D005814:Genes, Viral; D014644:Genetic Variation; D016679:Genome, Viral; D006023:Glycoproteins / Q000235:genetics; D017862:Phycodnaviridae / Q000235:genetics* / Q000472:pathogenicity; D014759:Viral Envelope Proteins / Q000235:genetics
D006023:Glycoproteins; D014759:Viral Envelope Proteins; C459403:Vp260 protein, chlorovirus