title: Antigen processing of the heptavalent pneumococcal conjugate vaccine carrier protein CRM(197) differs depending on the serotype of the attached polysaccharide.
abstract: The pneumococcal (Pn) conjugate vaccine includes seven different polysaccharides (PS) conjugated to CRM(197). Utilizing antigen-processing cells and a CRM(197)-specific mouse T-cell hybridoma, we found that the serotype of conjugated PnPS dramatically affected antigen processing of CRM(197). Unconjugated CRM(197) and serotype conjugates 14 and 18C were processed more efficiently.
authors.affiliation: Department of Pediatrics, Case Western Reserve University, Cleveland, Ohio 44106, USA.
authors.forename: Ethan G
authors.initials: EG
authors.lastname: Leonard
authors.forename: David H
authors.initials: DH
authors.lastname: Canaday
authors.forename: Clifford V
authors.initials: CV
authors.lastname: Harding
authors.forename: John R
authors.initials: JR
authors.lastname: Schreiber
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017951:Antigen Presentation; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000378:metabolism*; D015798:HLA-DR1 Antigen / Q000502:physiology; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D022242:Pneumococcal Vaccines / Q000008:administration & dosage*; D011135:Polysaccharides, Bacterial / Q000008:administration & dosage*; D012703:Serotyping; D013601:T-Lymphocytes / Q000276:immunology; D018074:Vaccines, Conjugate / Q000008:administration & dosage*
chemical_list: D001426:Bacterial Proteins; D015798:HLA-DR1 Antigen; D022242:Pneumococcal Vaccines; D011135:Polysaccharides, Bacterial; D018074:Vaccines, Conjugate; C028581:CRM197 (non-toxic variant of diphtheria toxin)