Nerve growth factor induces proliferation of PC12 cells through Cdc42.
Nerve growth factor (NGF) can change neurite outgrowth and cellular morphology of rat adrenal pheochromocytoma PC12 cells. In the present study, stable PC12 cells with constitutively active form (L61Cdc42), the dominant negative constructs (N17Cdc42), and wild type Cdc42 did not proliferate in low serum condition. However, on exposure to NGF, PC12 cells transfected with L61Cdc42 cDNA proliferated, whereas wild type Cdc42 and N17Cdc42 cDNA-transfected PC12 cells did not. When the cells were stimulated with NGF, ERK1/2 was transiently activated in L61Cdc42-transfected PC12 cells, whereas the activation of ERK1/2 was sustained in other cell lines. NGF repressed the induction of p21WAF1, cyclin-dependent kinase (CDK) inhibitor, in L61Cdc42 transfected PC12 cells, whereas NGF induced the expression of p21WAF1 in other PC12 cell lines.
Seo
Ji-Yeon
JY
Department of Biochemistry, College of Medicine, Hallym University, Chunchon, Ockchon-Dong 1, Kangwon-Do, Korea.
Kim
Jun-Sub
JS
Ghang
Jeon-Ho
JH
Kang
Tae-Cheon
TC
Suh
Jun-Gyo
JG
Kim
Eung-Gook
EG
Kim
Jong-Il
JI
Kim
Jaebong
J
Lee
Jae-Yong
JY
Park
Jae-Bong
JB
D000818:Animals; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D006801:Humans; D020932:Nerve Growth Factor / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects* / Q000378:metabolism; D016716:PC12 Cells; D051381:Rats; D020764:cdc42 GTP-Binding Protein / Q000096:biosynthesis* / Q000235:genetics
D020932:Nerve Growth Factor; D020764:cdc42 GTP-Binding Protein