title: RNA-binding protein HuR enhances p53 translation in response to ultraviolet light irradiation.
abstract: Exposure to short-wavelength UV light (UVC) strongly induces p53 expression. In human RKO colorectal carcinoma cells, this increase was not due to elevated p53 mRNA abundance, cytoplasmic export of p53 mRNA, or UVC-triggered stabilization of the p53 protein. Instead, p53 translation was potently enhanced after UVC irradiation. The 3' UTR of p53 was found to be a target of the RNA-binding protein HuR in a UVC-dependent manner in vitro and in vivo. HuR-overexpressing RKO cells displayed elevated p53 levels, whereas cells expressing reduced HuR showed markedly diminished p53 abundance and p53 translation. Our results demonstrate a role for HuR in binding to the p53 mRNA and enhancing its translation.
authors.affiliation: Laboratory of Cellular and Molecular Biology, National Institute on Aging-Intramural Research Program, National Institutes of Health, Baltimore, MD 21224, USA.
authors.forename: Krystyna
authors.initials: K
authors.lastname: Mazan-Mamczarz
authors.forename: Stefanie
authors.initials: S
authors.lastname: Galbán
authors.forename: Isabel
authors.initials: I
authors.lastname: López de Silanes
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Martindale
authors.forename: Ulus
authors.initials: U
authors.lastname: Atasoy
authors.forename: Jack D
authors.initials: JD
authors.lastname: Keene
authors.forename: Myriam
authors.initials: M
authors.lastname: Gorospe
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics*; D000954:Antigens, Surface; D019921:Biotinylation; D002277:Carcinoma / Q000473:pathology; D015179:Colorectal Neoplasms / Q000473:pathology; D018076:DNA, Complementary / Q000235:genetics; D051959:ELAV Proteins; D000067780:ELAV-Like Protein 1; D024202:Electrophoretic Mobility Shift Assay; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects; D016158:Genes, p53; D006801:Humans; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology; D011233:Precipitin Tests; D011485:Protein Binding; D014176:Protein Biosynthesis / Q000528:radiation effects*; D025941:Protein Interaction Mapping; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000502:physiology; D014162:Transfection; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis*; D014466:Ultraviolet Rays
chemical_list: D020413:3' Untranslated Regions; D000954:Antigens, Surface; D018076:DNA, Complementary; D051959:ELAV Proteins; D000067780:ELAV-Like Protein 1; C098770:ELAVL1 protein, human; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins; D016159:Tumor Suppressor Protein p53