title: Cell interaction with protein-loaded interpenetrating networks containing modified gelatin and poly(ethylene glycol) diacrylate.
abstract: The effects of modification to an interpenetrating network (IPN) system composed of gelatin and poly(ethylene glycol) diacrylate were characterized by protein release kinetics, fibroblast adhesion, and in vivo host response. The maximum cumulative percent of parvalbumin released from various IPN formulations ranged from 17.6+/-3.2% to 56.9+/-35.4% over 2-96h. Despite modifying gelatin with ethylenediaminetetraacetic dianhydride and/or monomethoxy poly(ethylene glycol) monoacetate ester or increasing the gelatin content, the largest amount of parvalbumin released occurred within 24h, prior to material bulk degradation. Over the time period evaluated, little (i.e. <1%) of the basic fibroblast growth factor (bFGF) loaded into the IPNs evaluated was released, independent of modifications made to the IPN formulation. Fibroblast adhesion to IPNs with or without loaded bFGF was quantified. The adherent fibroblast density on the IPNs was significantly lower than that of TCPS controls at all times independent of the IPN formulation tested and bFGF loading. Select IPN formulations elicited a comparable level of acute and chronic inflammatory response in vivo when compared with the gelatin and poly(ethylene glycol) diacrylate starting materials. IPNs provide a minimal cell-active surface that could be employed as delivery matrices and for further bioconjugation to mediate specific cellular response.
authors.affiliation: Department of Biomedical Engineering, College of Engineering, University of Wisconsin-Madison, Madison, WI, USA.
authors.forename: Jeanine A
authors.initials: JA
authors.lastname: Burmania
authors.forename: Kelly R
authors.initials: KR
authors.lastname: Stevens
authors.forename: Weiyuan John
authors.initials: WJ
authors.lastname: Kao
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D003432:Cross-Linking Reagents / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry; D005347:Fibroblasts / Q000378:metabolism; D005780:Gelatin / Q000737:chemistry*; D005976:Glutaral / Q000737:chemistry; D007249:Inflammation; D007700:Kinetics; D007962:Leukocytes / Q000378:metabolism; D008956:Models, Chemical; D010320:Parvalbumins / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry*; D013997:Time Factors
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D010320:Parvalbumins; D016222:Fibroblast Growth Factor 2; D011092:Polyethylene Glycols; D005780:Gelatin; D005976:Glutaral