Efforts toward creating unnatural base pairs for an expanded genetic code.
A series of unnatural base pairs was designed and examined for the expansion of the genetic alphabet and for a better understanding of the mechanism of nucleic acid biosyntheses. To improve the shape complementarity of the previously developed unnatural base pairs, 2-amino-6-(N,N-dimethylamino)purine (x)--pyridon-2-one (y) and 2-amino-6-(2-thienyl)purine (s)--y, the pyrimidine analogue, y, was replaced by a five-member ring, 4-imidazolin-2-one (z), and the s-z pairing in replication was examined. Unnatural bases based on the five-member ring were also applied to the development of non-hydrogen-bonded base pairs.
Hirao
I
I
Yokoyama CytoLogic Project, ERATO, JST, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
Mitsui
T
T
Fujiwara
T
T
Kimoto
M
M
To
T
T
Okuni
T
T
Sato
A
A
Harada
Y
Y
Yokoyama
S
S
D020029:Base Pairing; D004261:DNA Replication; D005815:Genetic Code; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D011685:Purine Nucleotides / Q000737:chemistry; D011742:Pyrimidine Nucleotides / Q000737:chemistry
D009841:Oligonucleotides; D011685:Purine Nucleotides; D011742:Pyrimidine Nucleotides