An efficient chemical method for dephosphorylation of phosphopeptides.
Phosphate moieties found on serine, threonine or tyrosine residues in peptides, e.g., in proteolytic digests of proteins, were cleaved using hydrofluoric acid or hydrogen fluoride-pyridine without side reactions.
Kuyama
Hiroki
H
Life Science Laboratory, Shimadzu Corporation, Kyoto 604-8511, Japan.
Toda
Chikako
C
Watanabe
Makoto
M
Tanaka
Koichi
K
Nishimura
Osamu
O
D000595:Amino Acid Sequence; D002364:Caseins / Q000378:metabolism; D006858:Hydrofluoric Acid / Q000737:chemistry* / Q000494:pharmacology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D010748:Phosphopeptides / Q000737:chemistry*; D010766:Phosphorylation / Q000187:drug effects; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014357:Trypsin / Q000378:metabolism
D002364:Caseins; D010446:Peptide Fragments; D010748:Phosphopeptides; D014357:Trypsin; D006858:Hydrofluoric Acid