Laminin-5 gamma2-chain expression and DNA ploidy as predictors of prognosis in endometrial carcinoma.
Expression of the laminin-5 gamma2-chain in carcinoma cells has been implicated in tumor invasion. The aim was to investigate the expression and prognostic significance of the ln-5 gamma2-chain compared with clinicopathological factors and tumor cell DNA ploidy in endometrial carcinoma. Histological specimens from 80 endometrial carcinomas were examined with respect to immunohistochemical ln-5 gamma2-chain expression and correlated to the clinicopathological characteristics, DNA ploidy, and survival. Sixty-eight of 80 investigated cases were judged to be positive for the ln-5 gamma2-chain. Ln-5 gamma2-chain did not show any correlation to stage, histopathological subtype, grade, and DNA ploidy. In univariate analyses, advanced stage (p < 0.001), nonendometrioid carcinoma (p = 0.030), low grade (p < 0.001), aneuploid tumors (p < 0.001), and ln-5 gamma2-chain expression (p = 0.017) were highly associated with poor survival. Aneuploid tumors in combination with strong ln-5 gamma2-chain expression were significant predictors (p < 0.001) of poor prognosis. In multivariate analyses including stage, histopathological subgroup, grade, DNA ploidy, and ln-5 gamma2-chain expression, all lost their significant prognostic information except for stage (p < 0.001) and grade (p < 0.05). Ln-5 gamma2-chain expression and DNA ploidy both as a single parameter and in combination were demonstrated to be signifi- cant prognostic factors in univariate analysis. However, stage and grade provided more useful clinical information beyond histopathological subgroup, DNA ploidy, and ln-5 gamma2-chain expression. The results also indicate that ln-5 gamma2-chain expression is upregulated during the progression of endometrial carcinoma.
Lundgren
C
C
Department of Gynaecologic Oncology, Radiumhemmet, Karolinska Hospital and Institute, S 171-76 Stockholm, Sweden. caroline.lundgren@ks.se
Frankendal
B
B
Silfverswärd
C
C
Nilsson
B
B
Tryggvason
K
K
Auer
G
G
Nordström
B
B
D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D004273:DNA, Neoplasm; D016889:Endometrial Neoplasms / Q000235:genetics* / Q000378:metabolism; D005260:Female; D006801:Humans; D015999:Multivariate Analysis; D011003:Ploidies; D011379:Prognosis; D000097794:Kalinin
D015815:Cell Adhesion Molecules; D004273:DNA, Neoplasm; D000097794:Kalinin