Interaction of cyanine dyes with nucleic acids: XXXI. Using of polymethine cyanine dyes for the visualization of DNA in agarose gels.
Fifteen polymethine cyanine dyes were studied as fluorescent stains for DNA in electrophoretic gels. Among studied cyanines, two dyes CPent V and CCyan 2-O most effectively visualized covalently closed and linear double-stranded DNA molecules in gels under standard conditions using UV-illumination, green filter and black-and-white photo film. Ethidium bromide was 1.2-1.6 times more effective as compared to cyanine dyes in staining of DNA in the concentration range of 8-18 ng, while studied cyanines were more sensitive to DNA quantity above 50 ng.
Matselyukh
B P
BP
Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, 154 Zabolotny Street, Kyiv 03143, Ukraine. bohdan@serv.imv.kiev.ua
Yarmoluk
S M
SM
Matselyukh
A B
AB
Kovalska
V B
VB
Kocheshev
I O
IO
Kryvorotenko
D V
DV
Lukashov
S S
SS
D002232:Carbocyanines / Q000737:chemistry*; D004247:DNA / Q000032:analysis / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D004996:Ethidium / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D015394:Molecular Structure; D009696:Nucleic Acids / Q000737:chemistry*; D013194:Staining and Labeling
D002232:Carbocyanines; D005456:Fluorescent Dyes; D009696:Nucleic Acids; D004247:DNA; D004996:Ethidium