title: Efficient liquid-phase synthesis of short-length nucleotides via the phosphoramidite method using stoichiometric amounts of reactants.
abstract: The condensation of a nucleoside phosphoramidite and a nucleoside by the aid of a suitable promoter in stoichiometric use is achieved in acetonitrile in the presence of molecular sieves 3A or 4A to give a desired coupling product in an excellent yield. This strategy is particularly useful for the large-scale synthesis of short nucleotides.
authors.affiliation: Laboratory of Bioorganic Chemistry, Graduate School of Human Informatics, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Hirata
authors.forename: J
authors.initials: J
authors.lastname: Sugimoto
authors.forename: R
authors.initials: R
authors.lastname: Kawai
authors.forename: M
authors.initials: M
authors.lastname: Kataoka
authors.forename: Y
authors.initials: Y
authors.lastname: Hayakawa
mesh_terms: D002625:Chemistry, Organic / Q000379:methods; D015224:Dideoxynucleosides / Q000138:chemical synthesis / Q000737:chemistry; D009705:Nucleosides / Q000737:chemistry; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000737:chemistry*
chemical_list: D015224:Dideoxynucleosides; D009705:Nucleosides; D009841:Oligonucleotides; D009943:Organophosphorus Compounds; C434331:phosphoramidite