title: Na(+)-dependent fructose transport via rNaGLT1 in rat kidney.
abstract: We found a system of Na(+)-dependent uptake of fructose by rat renal brush-border membrane vesicles. It consisted of two saturable components, and was thought to involve at least two transporters. rNaGLT1, a novel glucose transporter in rat kidney, showed fructose uptake as well as alpha-methyl-D-glucopyranoside uptake by transfected HEK293 cells. The features of the lower affinity type of fructose transporter in the brush-border membranes, such as affinity and substrate recognition, were very comparable with those of rNaGLT1-transfected HEK293 cells. These results indicated that rNaGLT1 is a primary fructose transporter in rat renal brush-border membranes.
authors.affiliation: Department of Pharmacy, Kyoto University Hospital, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan.
authors.forename: Naoshi
authors.initials: N
authors.lastname: Horiba
authors.forename: Satohiro
authors.initials: S
authors.lastname: Masuda
authors.forename: Chiharu
authors.initials: C
authors.lastname: Ohnishi
authors.forename: Daisuke
authors.initials: D
authors.lastname: Takeuchi
authors.forename: Masahiro
authors.initials: M
authors.lastname: Okuda
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Inui
mesh_terms: D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D005632:Fructose / Q000378:metabolism*; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D008871:Microvilli / Q000378:metabolism; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D012964:Sodium / Q000378:metabolism*
chemical_list: D009004:Monosaccharide Transport Proteins; D011994:Recombinant Proteins; D005632:Fructose; D012964:Sodium