Photo-regulation of RNA polymerase reaction by use of modified DNA carrying an azobenzene.
Transcription reaction by T7-RNA polymerase was photo-regulated on the basis of two strategies as depicted in Scheme 1. It was found that incorporation reaction of azobenzene-tethered uridine triphosphate proceeded only when azobenzene took trans-form (Scheme 1(A)). On the other hand, the transcription was more efficient when the azobenzene moiety, tethered to the non-template strand of the promoter DNA, was in its cis-form under UV irradiation (Scheme 1(B)). Thus, azobenzene-tethered DNAs are promising for the photo-regulation of gene-expression.
Asanuma
H
H
Research Center for Advanced Science and Technology, Univ. of Tokyo, Komaba, Meguro-ku, Tokyo 153-8904, Japan.
Tamaru
D
D
Yoshida
T
T
Yamazawa
A
A
Mitsui
T
T
Okuni
T
T
Kimoto
M
M
Hirao
I
I
Yokoyama
S
S
Komiyama
M
M
D001391:Azo Compounds / Q000737:chemistry*; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D005786:Gene Expression Regulation; D008957:Models, Genetic; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic / Q000528:radiation effects*; D014466:Ultraviolet Rays; D014544:Uridine Triphosphate / Q000737:chemistry; D014764:Viral Proteins
D001391:Azo Compounds; D014764:Viral Proteins; D004247:DNA; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases; C009850:azobenzene; D014544:Uridine Triphosphate