title: Effects of a 50 Hz sinusoidal magnetic field on cell adhesion molecule expression in two human osteosarcoma cell lines (MG-63 and Saos-2).
abstract: The possibility that a sinusoidal 50 Hz magnetic field with a magnetic flux density of 0.5 mT can induce variations in the expression of cell adhesion molecules (CAMs) in two human osteosarcoma cell lines (MG-63 and Saos-2) was investigated. In particular, the expression of two important integrins, VLA-2, the receptor for collagen, and VLA-5, the receptor for fibronectin, as well as CD44, were examined in both cell lines after these had been exposed for 7 and 14 days to a 50 Hz, 0.5 mT field. Cell surface morphology (scanning electron microscopy), cell growth characteristics (growth curves and cell cycle phase distribution), and cell death (necrosis and apoptosis) were also examined. The results demonstrate that no variations in surface morphology and cell death occurred between control and exposed cells in both MG-63 and Saos-2 cells, while significant changes were noted in cell growth and fibronectin and CD44 expression in MG-63 cells. The results are discussed in view of the important role that CAMs play in controlling various cancer cell functions, particularly proliferation and metastasis.
authors.affiliation: Laboratorio di Ultrastrutture, Istituto Superiore di Sanità, Rome, Italy. santini@iss.it
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Santini
authors.forename: Gabriella
authors.initials: G
authors.lastname: Rainaldi
authors.forename: Antonella
authors.initials: A
authors.lastname: Ferrante
authors.forename: Pietro Luigi
authors.initials: PL
authors.lastname: Indovina
authors.forename: Paolo
authors.initials: P
authors.lastname: Vecchia
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Donelli
mesh_terms: D017209:Apoptosis / Q000528:radiation effects; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002455:Cell Division / Q000528:radiation effects; D045744:Cell Line, Tumor / Q000378:metabolism / Q000528:radiation effects; D048429:Cell Size / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D004560:Electricity; D004574:Electromagnetic Fields; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects*; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism*; D038982:Integrin alpha2beta1 / Q000378:metabolism*; D039081:Integrin alpha5beta1 / Q000378:metabolism*; D012516:Osteosarcoma / Q000378:metabolism* / Q000473:pathology*; D011829:Radiation Dosage
chemical_list: D015815:Cell Adhesion Molecules; D018960:Hyaluronan Receptors; D038982:Integrin alpha2beta1; D039081:Integrin alpha5beta1