A rapid and sensitive liquid chromatography/tandem mass spectrometry method for determination of docetaxel in human plasma.
A novel, rapid and sensitive isocratic liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed for quantification of docetaxel in human plasma with paclitaxel as internal standard. The high sensitivity and specificity of MS/MS detection enabled the use of a small volume of plasma (0.05 mL) and a simple liquid-liquid extraction procedure. Furthermore, a very short run-time (3 min) fulfilled the need for monitoring plasma levels of docetaxel from large-scale clinical studies. The calibration curve for docetaxel was linear over the range 5-1000 ng/mL with coefficients of correlation >0.999 using only 0.05 mL plasma. The intra- and inter-day precisions (CV) of analysis were <7%, and accuracy ranged from 96 to 110%. The applicability of the method was demonstrated in a pharmacokinetic study of a 1-h infusion of docetaxel with dosages of 75 mg/m(2). Possible conjugated metabolites of docetaxel were not detected in patients' samples.
Wang
L Z
LZ
The Cancer Institute, National Health Group, Singapore.
Goh
B C
BC
Grigg
M E
ME
Lee
S C
SC
Khoo
Y M
YM
Lee
H S
HS
D000972:Antineoplastic Agents, Phytogenic / Q000097:blood*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D000077143:Docetaxel; D006801:Humans; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D017239:Paclitaxel / Q000031:analogs & derivatives* / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results; D043823:Taxoids
D000972:Antineoplastic Agents, Phytogenic; D007202:Indicators and Reagents; D043823:Taxoids; D000077143:Docetaxel; D017239:Paclitaxel