2',4'-BNA bearing unnatural nucleobases: toward the expansion of the target sequence of double-stranded DNA in triplex formation.
It is absolutely necessary for practical application of antigene strategy to create nucleoside analogues recognizing pyrimidine.purine interruption in dsDNA. To develop a nucleoside analogue to interact with T.A interruption, we designed and synthesized a novel 2',4'-BNA monomer (HBB), 2-(2'-O,4'-C-methyleneribofuranosyl)phenol, and it was introduced into a triplex-forming oligonucleotide (TFO). On melting temperature (Tm) measurements, HBB was found to interact with T.A interruption with moderate binding affinity, and unprecedented dU.A base pair recognition ability of HBB was also observed.
Obika
S
S
Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
Hari
Y
Y
Inohara
H
H
Imanishi
T
T
D001483:Base Sequence; D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry*; D009705:Nucleosides / Q000737:chemistry; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D010636:Phenols / Q000737:chemistry*; D012266:Ribose / Q000031:analogs & derivatives / Q000737:chemistry*; D013696:Temperature
C476359:2-(2'-O,4'-C-methyleneribofuranosyl)phenol; D009705:Nucleosides; D009841:Oligonucleotides; D010636:Phenols; C051802:triplex DNA; D012266:Ribose; D004247:DNA