title: Identification of oxidation product of arbutin in mushroom tyrosinase assay system.
abstract: In order to elucidate whitening mechanisms of arbutin (hydroquinone-O-beta-D-glucopyranoside), its effects on mushroom tyrosinase were analyzed by spectrophotometric, polarographic, and HPLC experiments. It was found that as soon as catalytic amounts of L-DOPA become available as a cofactor, arbutin acts as a monophenol substrate. A significant enzymatic product was identified as 3,4-dihydroxyphenyl-O-beta-D-glucopyranoside by NMR and MS experiments.
authors.affiliation: Department of Environmental Science, Policy and Management, University of California, CA 94720-3112, Berkeley, USA.
authors.forename: Ken ichi
authors.initials: Ki
authors.lastname: Nihei
authors.forename: Isao
authors.initials: I
authors.lastname: Kubo
mesh_terms: D000363:Agaricales / Q000201:enzymology*; D001104:Arbutin / Q000737:chemistry*; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D007700:Kinetics; D007980:Levodopa / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D014442:Monophenol Monooxygenase / Q000378:metabolism*; D010084:Oxidation-Reduction; D010101:Oxygen Consumption / Q000187:drug effects; D011048:Polarography; D013056:Spectrophotometry, Ultraviolet
chemical_list: D007980:Levodopa; D001104:Arbutin; D014442:Monophenol Monooxygenase