[Differentially expressed genes associated with cold acclimation].
To investigate the upregulated genes associated with cold acclimation, a cold acclimation model was established based on Balb/C mouse. mRNA of muscle and liver were isolated, and the upregulated genes of these tissues were studied by representational differential analysis (RDA). The upregulated genes then were sequenced and searched by Blast software in GenBank database. The results showed that some genes were upregulated and possibly associated with cold acclimation. Three of these genes, transferrin, fibrinogen B-beta-chains and a new gene fragment (Genbank ID: AF454762), were confirmed to be upregulated by RNA slot-blot analysis. The finding of these genes might contribute to further understanding of the molecular mechanisms of cold acclimation.
Yang
Fa-Qing
FQ
Institute of Hygiene and Environment Medicine, Tianjin 300050.
Qian
Ling-Jia
LJ
Wang
Wan-Yin
WY
Ren
Hui-Rong
HR
Xu
Da
D
D000064:Acclimatization / Q000235:genetics*; D000818:Animals; D003080:Cold Temperature; D015870:Gene Expression; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018482:Muscle, Skeletal / Q000378:metabolism; D059467:Transcriptome; D015854:Up-Regulation