Complete DNA sequence and analysis of two cryptic plasmids isolated from Lactobacillus plantarum.
The complete nucleotide sequence of two cryptic plasmids isolated from Lactobacillus plantarum strain AS1.2986 has been determined. The smaller plasmid, designated pLP2000, encodes a 37.0kDa Rep protein and has a 17bp sequence repeated 10 times. Sequence analysis of the larger plasmid, designated pLP9000, revealed nine putative open reading frames (ORFs). Based on sequence similarity, ORF1 codes for a putative magnesium transporter protein that shows similarities to CorA from plasmid pCIS3 (Lactococcus lactis). None of the nine ORFs shows similarity to any known Rep protein. Southern blot analysis indicates these two plasmids both replicate via a rolling circle (RC) mechanism.
Daming
Ren
R
State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, Shanghai 200433, People's Republic of China. dmren@fudan.edu.cn
Yinyu
Wang
W
Zilai
Wang
W
Jun
Cui
C
Hekui
Lan
L
Jingye
Zhou
Z
D014644:Genetic Variation; D007778:Lactobacillus / Q000235:genetics*; D013294:Lactococcus lactis / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid