Effect of acetic acid added to cooking water on the dissolution of proteins and activation of protease in rice.
The effect of acetic acid on the dissolution of proteins in rice was studied to elucidate the mechanism for the textural change induced by the acid by chemical and SDS-PAGE analyses of the rice proteins in the soaking solution. More proteins were extracted with 0.2 M acetic acid (pH 2.7) than with water (pH 6.8). The effect of acetic acid on the protein dissolution increased with increasing temperature. Immunoblotting confirmed that, when rice was soaked in acetic acid, glutelin was dissolved into the soaking solution and degraded by aspartic proteinase. Aspartic proteinase degraded glutelin much more than it did albumin and globulin. It was found that the combined amount of albumin and globulin dissolved into the acetic acid solution was much larger than that of glutelin, despite the smaller amounts present of albumin and globulin than of glutelin. Metal ions were extracted more with acetic acid than with water. In addition, carboxypeptidase was activated under the acidic condition and resulted in an increase in the amount of free amino acids. The main effect of acetic acid on the dissolution of rice proteins was enhancement of the solubility of albumin, globulin, and glutelin, the effect of proteases being minor.
Ohishi
Kyoko
K
School of Human Life and Environmental Science, Ochanomizu University, Otsuka 2-1-1, Bunkyo-ku, Tokyo 112-8610, Japan. kyohishi@h3.dion.ne.jp
Kasai
Midori
M
Shimada
Atsuko
A
Hatae
Keiko
K
D019342:Acetic Acid / Q000494:pharmacology*; D000596:Amino Acids / Q000032:analysis; D016282:Aspartic Acid Endopeptidases / Q000378:metabolism; D002268:Carboxypeptidases / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D005983:Glutens / Q000378:metabolism; D006358:Hot Temperature; D008670:Metals / Q000032:analysis; D009842:Oligopeptides / Q000032:analysis; D012275:Oryza / Q000737:chemistry*; D010940:Plant Proteins / Q000032:analysis / Q000378:metabolism*; D012996:Solutions; D014867:Water
D000596:Amino Acids; D008670:Metals; D009842:Oligopeptides; D010940:Plant Proteins; D012996:Solutions; D014867:Water; D005983:Glutens; D002268:Carboxypeptidases; D010450:Endopeptidases; D016282:Aspartic Acid Endopeptidases; D019342:Acetic Acid