Isolation and functional analysis of the melanoma specific promoter region of human GD3 synthase gene.
Human GD3 synthase gene consisted of five exons and span about 135 kilobases. The 5'-flanking region lacked canonical TATA and CAAT boxes, but contained SP1 binding site(s) as in rat and mouse. The promoter activity in the 5'-flanking region (-2262 approximately +1) became definite when SV40 enhancer was added to the reporter plasmid. Luciferase assay with deletion mutants suggested the existence of a silencer region between -2262 and -978 nt similarly with those in mouse and rat. They also commonly contained a GT/CG repeat sequence at upstream of -1200 approximately -1300 nt, suggesting that they form Z-type DNA, and are involved in the gene regulation.
Furukawa
Keiko
K
Department of Biochemistry II, Nagoya University School of Medicine, 65 Tsurumai, Showa, Nagoya 466-0065, Japan. koichi@med.nagoya-u.ac.jp
Horie
Masato
M
Okutomi
Kei-ichi
K
Sugano
Sumio
S
Furukawa
Koichi
K
D024506:5' Flanking Region; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D004742:Enhancer Elements, Genetic / Q000235:genetics; D005091:Exons; D006801:Humans; D007438:Introns; D008545:Melanoma / Q000201:enzymology / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D012799:Sialyltransferases / Q000235:genetics* / Q000378:metabolism; D016329:Sp1 Transcription Factor / Q000378:metabolism; D016385:TATA Box; D024363:Transcription Initiation Site; D014407:Tumor Cells, Cultured
D016329:Sp1 Transcription Factor; D012799:Sialyltransferases; C043662:alpha-N-acetylneuraminate alpha-2,8-sialyltransferase