title: Three-dimensional structure and survival of newly formed blood vessels after focal cerebral ischemia.
abstract: Penumbra tissue becomes highly angiogenic after ischaemic stroke in man, and the re-establishment of a functional vasculature might be beneficial to patients. Unilateral ischaemia was induced in male Sprague-Dawley rats by permanent occlusion of the distal left middle cerebral artery (MCAO). Animals with stroke were kept alive for 1, 7, 14, 21 or 28 days after which time they were terminally anaesthetized. Vascular casts of infarcted areas, analyzed by scanning electron microscopy demonstrated that radially arranged neocortical arterioles and venules lost their regular patterns within one day of occlusion, and soon afterwards started to form a very dense network of anastomosing microvessels. At 1 week, vascular budding was visible at many sites. The smallest microvessels (4-10 microm) formed connections with the surrounding proliferating vessels similar to those in the normal brain. Survival of microvascular endothelial cells (ECs) was studied by double labeling of tissue sections using immunohistochemistry and antibodies to caspase-3, and TUNEL staining for apoptotic cells. ECs demonstrated intensive staining for caspase-3 and also staining by TUNEL, particularly near the infarct border, 14 days post-MCAO. These data support the hypothesis that growing blood vessels in ischaemic tissue form new connections, the pattern of which is similar to that in normal rat brain, but different to those formed in growing tumours. This normal growth pattern might be essential in future therapies involving induction of vascularization and neuroprotection to enhance long-term survival of the penumbra.
authors.affiliation: Department of Neurology, Hospital Universitario de Bellvitge, Barcelona, Spain. krupinski@medicina.ub.es
authors.forename: Jerzy
authors.initials: J
authors.lastname: Krupinski
authors.forename: Paul
authors.initials: P
authors.lastname: Stroemer
authors.forename: Mark
authors.initials: M
authors.lastname: Slevin
authors.forename: Eulalia
authors.initials: E
authors.lastname: Marti
authors.forename: Pat
authors.initials: P
authors.lastname: Kumar
authors.forename: Francisco
authors.initials: F
authors.lastname: Rubio
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001808:Blood Vessels / Q000503:physiopathology* / Q000648:ultrastructure; D002545:Brain Ischemia / Q000473:pathology* / Q000503:physiopathology*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002478:Cells, Cultured; D053938:DNA Fragmentation; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D020287:In Situ Nick-End Labeling / Q000379:methods; D020244:Infarction, Middle Cerebral Artery / Q000503:physiopathology; D008297:Male; D018625:Microscopy, Atomic Force / Q000295:instrumentation / Q000379:methods; D010477:Perfusion / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury; D013997:Time Factors; D014841:von Willebrand Factor / Q000378:metabolism
chemical_list: D014841:von Willebrand Factor; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases