Growth hormone expression in ontogenic development in gilthead sea bream.
The pattern of expression of the growth hormone (GH) gene was studied during the early development of gilthead sea bream ( Sparus aurata). The GH transcript was detected from the 2nd day of the larval stage onwards. In the next stages the expression level fluctuated, possibly due to different regulatory factors. The distribution of GH mRNA studied by in situ hybridization (ISH) was found to be pituitary specific. Hybridization signals for GH mRNA were detected for the first time in 4-day-old larvae. Throughout development the cells that express GH mRNA were mainly located in the proximal pars distalis. Mammosomatotroph cells coexpressing GH and PRL were not detected in juveniles or adults. Moreover, the possible involvement of GH in asynchronic growth in cultivation of gilthead sea bream was also examined by ISH. No differences in the distribution of GH cells were observed in the three sizes of juveniles of gilthead sea bream studied. These results suggest that the transcription of GH is involved in the early developmental stages of sea bream and the asynchronous growth-related changes are not due to distinct distribution of GH cells.
Herrero-Turrión
M J
MJ
Unidad de Biología Celular, Instituto de Neurociencias de Castilla y León, Facultad de Medicina, Universidad de Salamanca, 37007, Salamanca, Spain.
Rodríguez
R E
RE
Velasco
A
A
González-Sarmiento
R
R
Aijón
J
J
Lara
J M
JM
D000818:Animals; D002847:Chromatography, Agarose; D004625:Embryo, Nonmammalian / Q000737:chemistry / Q000378:metabolism; D047108:Embryonic Development; D029941:Fish Proteins; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006023:Glycoproteins / Q000032:analysis / Q000235:genetics; D013006:Growth Hormone / Q000032:analysis / Q000235:genetics*; D006651:Histocytochemistry; D017403:In Situ Hybridization / Q000379:methods; D007814:Larva / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism; D010902:Pituitary Gland / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D010903:Pituitary Gland, Anterior / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D010907:Pituitary Hormones / Q000032:analysis / Q000235:genetics; D011388:Prolactin / Q000032:analysis / Q000235:genetics; D012313:RNA / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D021541:Sea Bream / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*
D029941:Fish Proteins; D006023:Glycoproteins; D010907:Pituitary Hormones; C064448:somatolactin protein, fish; D012313:RNA; D011388:Prolactin; D013006:Growth Hormone; D005634:Fructose-Bisphosphate Aldolase