Quantification of neuroepithelial bodies and their innervation in fawn-hooded and Wistar rat lungs.
The Fawn-Hooded rat (FHR), a model for primary pulmonary hypertension, shows an unexplained hypersensitivity to airway hypoxia. Because pulmonary neuroepithelial bodies (NEBs) appear to express a functional oxygen-sensing mechanism and an extensive sensory innervation, possible changes in this system should be taken into consideration. In the present study a comparative analysis of NEBs and their selective innervation was performed in FHRs and Wistar control rats. In both rat strains, the number of NEBs was estimated to be around 3,500, approximately 40% of which were innervated by vagal sensory calbindin D28k-immunoreactive (IR) nerve endings and approximately 50% by spinal sensory calcitonin gene-related peptide (CGRP)-IR nerve terminals. The number of intrinsic pulmonary nitrergic neurons and the percentage of pulmonary NEBs revealing a nitrergic innervation were highly significantly lower in FHRs. In both FHRs and Wistar rats, a remarkable morphologic interaction was observed between the intrinsic nitrergic and the CGRP-IR sensory population contacting NEBs. Our findings suggest a possible link between the hypersensitivity to airway hypoxia observed in FHRs and a reduced intrinsic pulmonary nitrergic innervation, possibly via the interaction with pulmonary NEBs and their spinal sensory CGRP-IR innervation.
Van Genechten
Jeroen
J
Department of Biomedical Sciences, University of Antwerp-RUCA, Groenenborgerlaan 171, B-2020 Antwerp, Belgium.
Brouns
Inge
I
Burnstock
Geoff
G
Timmermans
Jean-Pierre
JP
Adriaensen
Dirk
D
D000818:Animals; D004847:Epithelial Cells / Q000166:cytology; D005260:Female; D007150:Immunohistochemistry; D008168:Lung / Q000294:innervation*; D008297:Male; D026602:Nitrergic Neurons / Q000166:cytology*; D051381:Rats; D017208:Rats, Wistar; D013045:Species Specificity