Interactions of chlorophyllin with acridine orange, quinacrine mustard and doxorubicin analyzed by light absorption and fluorescence spectroscopy.
The present study was designed to estimate the ability of chlorophyllin (CHL) to interact with two acridine mutagens, quinacrine mustard (QM) and acridine orange (AO), and with the antitumor anthracycline doxorubicin (Dox). To this end, aqueous solutions of QM, AO or Dox during titration with CHL were subjected to spectrophotometry and spectrofluorimetry to detect possible interactions between these reagents. The data indicate that CHL forms complexes with AO, QM or Dox in these solutions. The presence of the complexes was manifested by a bathochromic shift of the absorption spectra, as well as by strong quenching of the fluorescence of each of these mutagens in the presence of CHL. CHL, thus, may serve as an interceptor of these mutagenic acridines in different in vivo or in vitro applications. Its ability to interact with Dox may potentially be utilized to detoxify patients overdosed with this or similar drugs.
Pietrzak
Monika
M
Department of Physics and Biophysics, University of Warmia and Mazury in Olsztyn, Oczapowskiego 4, 10-719 Olsztyn, Poland.
Wieczorek
Zbigniew
Z
Stachelska
Alicja
A
Darzynkiewicz
Zbigniew
Z
D000165:Acridine Orange / Q000737:chemistry / Q000378:metabolism*; D016587:Antimutagenic Agents / Q000737:chemistry*; D002735:Chlorophyllides / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D015394:Molecular Structure; D009153:Mutagens / Q000737:chemistry* / Q000378:metabolism; D011797:Quinacrine Mustard / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry
D016587:Antimutagenic Agents; D002735:Chlorophyllides; D009153:Mutagens; D011797:Quinacrine Mustard; D004317:Doxorubicin; C007020:chlorophyllin; D000165:Acridine Orange