title: High-throughput screening of mutant alpha-amylase libraries for increased activity at 129 degrees C.
authors.affiliation: Process development Biochemistry, Aventis Pharma Deutschland GmbH, Frankfurt, Germany.
authors.forename: Holger
authors.initials: H
authors.lastname: Berk
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Lebbink
mesh_terms: D003001:Cloning, Molecular; D019020:Directed Molecular Evolution / Q000379:methods; D004795:Enzyme Stability; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D014644:Genetic Variation; D007202:Indicators and Reagents; D019151:Peptide Library; D016133:Polymerase Chain Reaction / Q000379:methods; D020027:Pyrococcus furiosus / Q000201:enzymology / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013816:Thermodynamics; D000516:alpha-Amylases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D007202:Indicators and Reagents; D019151:Peptide Library; D011994:Recombinant Proteins; D000516:alpha-Amylases