Quantitative enantiomeric analysis of chlorcyclizine, hydroxyzine, and meclizine by capillary electrophoresis.
A simple capillary zone electrophoresis method was developed for the quantitative enantiomeric analysis of piperazine antihistamines with teratogenic suspicion in animals. Enantioseparation of chlorcyclizine, hydroxyzine, and meclizine was performed in glycine buffer (0.6 mol L(-1); pH 3.00) with sulfated beta-cyclodextrin (5 mg mL(-1)) as a chiral selector; and the separated drugs were monitored by ultra-violet detector. The lower quantitation of the individual enantiomer is attainable at 10 micro mol L(-1), using an achiral piperazine drug (cyclizine) as internal standard. The method is simple and rapid with a short run time (<5 min) for the analysis of chlorcyclizine, hydroxyzine or meclizine enantiomers.
Ho
Yu- Hsiang
Y
Graduate Institute of Pharmaceutical Sciences, Kaohsiung Medical University, 807, Kaohsiung, Taiwan.
Wu
Hsin- Lung
H
Wu
Shou- Mei
S
Chen
Su- Hwei
S
Kou
Hwang- Shang
H
D000818:Animals; D002021:Buffers; D002138:Calibration; D019075:Electrophoresis, Capillary / Q000379:methods*; D005998:Glycine; D006634:Histamine H1 Antagonists / Q000032:analysis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D006919:Hydroxyzine / Q000032:analysis* / Q000737:chemistry; D008468:Meclizine / Q000032:analysis* / Q000737:chemistry; D015394:Molecular Structure; D010879:Piperazines / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013237:Stereoisomerism
D002021:Buffers; D006634:Histamine H1 Antagonists; D010879:Piperazines; D006919:Hydroxyzine; D008468:Meclizine; C084597:chlorcyclizine; D005998:Glycine