title: An association between the radiation-induced arrest of G2-phase cells and low-dose hyper-radiosensitivity: a plausible underlying mechanism?
abstract: The survival of asynchronous and highly enriched G1-, S- and G2-phase populations of Chinese hamster V79 cells was measured after irradiation with 60Co gamma rays (0.1-10 Gy) using a precise flow cytometry-based clonogenic survival assay. The high-dose survival responses demonstrated a conventional relationship, with G2-phase cells being the most radiosensitive and S-phase cells the most radioresistant. Below 1 Gy, distinct low-dose hyper-radiosensitivity (HRS) responses were observed for the asynchronous and G2-phase enriched cell populations, with no evidence of HRS in the G1- and S-phase populations. Modeling supports the conclusion that HRS in asynchronous V79 populations is explained entirely by the HRS response of G2-phase cells. An association was discovered between the occurrence of HRS and the induction of a novel G2-phase arrest checkpoint that is specific for cells that are in the G2 phase of the cell cycle at the time of irradiation. Human T98G cells and hamster V79 cells, which both exhibit HRS in asynchronous cultures, failed to arrest the entry into mitosis of damaged G2-phase cells at doses less than 30 cGy, as determined by the flow cytometric assessment of the phosphorylation of histone H3, an established indicator of mitosis. In contrast, human U373 cells that do not show HRS induced this G2-phase checkpoint in a dose-independent manner. These data suggest that HRS may be a consequence of radiation-damaged G2-phase cells prematurely entering mitosis.
authors.affiliation: Radiation Biology Group, Karmanos Cancer Institute, Wayne State University, Detroit, Michigan 48201-2013, USA. marplesb@kci.wayne.edu
authors.forename: B
authors.initials: B
authors.lastname: Marples
authors.forename: B G
authors.initials: BG
authors.lastname: Wouters
authors.forename: M C
authors.initials: MC
authors.lastname: Joiner
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000528:radiation effects*; D002460:Cell Line; D002469:Cell Separation; D003037:Cobalt Radioisotopes; D006224:Cricetinae; D004307:Dose-Response Relationship, Radiation; D005434:Flow Cytometry; D016195:G2 Phase / Q000528:radiation effects*; D006801:Humans; D016196:S Phase; D014407:Tumor Cells, Cultured
chemical_list: D003037:Cobalt Radioisotopes