[Antimitogenic effect of Pygeum africanum extracts on human prostatic cancer cell lines and explants from benign prostatic hyperplasia].
OBJECTIVE
To analyze the effect of Pygeum africanum extracts on the in vitro proliferation of human prostate cells.


METHODS
Prostate cancer cell lines and benign prostatic hyperplasia derived epithelial cells were cultured and treated with P. africanum extracts. The effect on cell proliferation was monitored by H3-thymidine and bromodeoxyuridine uptake and flow cytometry assays.


RESULTS
The incubation with P. africanum extracts, with or without addition of amino acids, significantly and in a dose-dependent manner inhibits the proliferation of prostate cancer derived cells LnCaP, PZ-HPV-7, and CA-HPV-10. In the PZ-HPV-7 cells P. africanum extracts counteract the mitogenic action of EGF and block the transition from G1 to S in the cell cycle. P. africanum extracts also exert a potent antimitogenic action on the epithelial cells derived from benign prostatic hyperplasia explants.


CONCLUSIONS
The ethanolic P. africanum extracts have an antimitogenic effect on prostate cancer cells and benign prostatic hyperplasia epithelial cells. Such effect is associated with the inhibition of the mitogenic action of EGF, and it is accompanied by a decrease of cells entering the S Phase of the cell cycle.
Santa María Margalef
Anna
A
Unitat de Recerca Biomèdica, Hospital Vall d'Hebrón, Barcelona, España.
Paciucci Barzanti
Rosanna
R
Reventós Puigjaner
Jaume
J
Morote Robles
Juan
J
Thomson Okatsu
Timothy M
TM
D002455:Cell Division / Q000187:drug effects; D002461:Cell Line, Transformed / Q000187:drug effects; D002472:Cell Transformation, Viral; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D004261:DNA Replication / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D004847:Epithelial Cells / Q000187:drug effects; D000431:Ethanol; D005434:Flow Cytometry; D006131:Growth Inhibitors / Q000494:pharmacology*; D006801:Humans; D007633:Keratins / Q000032:analysis; D008297:Male; D008938:Mitosis / Q000187:drug effects*; D009363:Neoplasm Proteins / Q000032:analysis; D009924:Organ Culture Techniques; D027383:Papillomaviridae / Q000502:physiology; D010936:Plant Extracts / Q000494:pharmacology*; D011467:Prostate / Q000166:cytology / Q000187:drug effects*; D011470:Prostatic Hyperplasia / Q000473:pathology*; D011471:Prostatic Neoplasms / Q000473:pathology*; D000068218:Prunus africana; D017154:Stromal Cells / Q000187:drug effects; D014407:Tumor Cells, Cultured / Q000187:drug effects
D016895:Culture Media, Serum-Free; D006131:Growth Inhibitors; D009363:Neoplasm Proteins; D010936:Plant Extracts; D000431:Ethanol; D007633:Keratins