Synthesis and characterization of oligodeoxyribonucleotides carrying arrays of intercalating groups.
A novel strategy for attaching the functional groups to oligodeoxyribonucleotides (ODNs) is described. A thymidine carrying anthraquinone derivative at the N3 position (AnT) was synthesized and introduced into ODNs using the phosphoramidite method on a DNA synthesizer. Py-Pu-Py type triplex formation of ODNs was enhanced by attaching AnT to the 5'-end of the third strand.
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Department of Chemistry, Faculty of Science, Tokyo Metropolitan University, 1-1 Minamiohsawa, Hachioji, Tokyo 192-0397, Japan.
D000880:Anthraquinones / Q000737:chemistry; D001483:Base Sequence; D004247:DNA / Q000737:chemistry; D007364:Intercalating Agents / Q000737:chemistry*; D009705:Nucleosides / Q000737:chemistry; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000737:chemistry; D013936:Thymidine / Q000031:analogs & derivatives
D000880:Anthraquinones; D007364:Intercalating Agents; D009705:Nucleosides; D009838:Oligodeoxyribonucleotides; D009943:Organophosphorus Compounds; C434331:phosphoramidite; C051802:triplex DNA; D004247:DNA; D013936:Thymidine