Environmental isolates of Aeromonas spp. harboring the cagA-like gene of Helicobacter pylori.
We investigated the presence of cagA-like gene of Helicobacter pylori in environmental isolates of Aeromonas spp. from different water samples of Calcutta, India, by colony hybridization using a cagA-specific DNA probe and by PCR with cagA-specific primers. Nucleotide sequencing of five PCR products revealed 97 to 98% homology to canonical cagA of H. pylori 26695 as well as to four clinical H. pylori strains from Calcutta. The cagA-like gene of the environmental isolates was unstable in laboratory conditions and tended to be lost upon subculturing.
Datta
Simanti
S
National Institute of Cholera and Enteric Diseases, Beliaghata, Calcutta 700 010, India.
Khan
Asis
A
Nandy
Ranjan K
RK
Rehman
Motiur
M
Sinha
Sutapa
S
Chattopadhyay
Santanu
S
Das
Suresh C
SC
Nair
G Balakrish
GB
D000333:Aeromonas / Q000235:genetics* / Q000302:isolation & purification*; D000942:Antigens, Bacterial / Q000737:chemistry / Q000235:genetics*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D005618:Fresh Water / Q000382:microbiology*; D016481:Helicobacter Infections / Q000382:microbiology; D016480:Helicobacter pylori / Q000235:genetics; D006801:Humans; D007194:India; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C080866:cagA protein, Helicobacter pylori