title: [Evaluation of construction and expression shiga toxin2 A-luteinizing hormone releasing hormone recombinant toxin].
abstract: OBJECTIVE
  The purpose is to construct shiga toxin2A-luteinizing hormone releasing hormone (Stx2A-LHRH) recombinant toxin which can kill cancer cells specifically and try to get a new target-binding drug.
  
  
  METHODS
  The fragment of Stx2A-LHRH DNA amplified by polymerase chain reaction (PCR) were cloned into plasmid pET-20b(+) vector. The recombinant plasmid pET-Stx2A-LHRH was constructed successfully and identified by endonucleases digestion and sequencing analysis then it was transformed to Escherichia coli BL21 (DE3) and expressed under the induction of isopropyl-beta-D-thiogalactopyranoside.
  
  
  RESULTS
  The result of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and gel thin layer scanning proved a special band of a molecular weight of about 38,000, accounting for 10.32% of total amount of the supernatant protein. Stx2A-LHRH recombinant toxin can kill HeLa cells clearly.
  
  
  CONCLUSIONS
  Stx2A-LHRH recombinant toxin may become a choice of target-binding drug in the future.
authors.affiliation: Department of Obstetrics and Gynecology, First Hospital Jilin University, Changchun 130021, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yue
authors.forename: Xiao-wei
authors.initials: XW
authors.lastname: Yu
authors.forename: Yu-huan
authors.initials: YH
authors.lastname: Yue
authors.forename: Shu-zhang
authors.initials: SZ
authors.lastname: Feng
authors.forename: Ping
authors.initials: P
authors.lastname: Zhu
authors.forename: He-lian
authors.initials: HL
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Yue
mesh_terms: D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D007987:Gonadotropin-Releasing Hormone / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D011956:Receptors, Cell Surface / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D022641:Shiga Toxin 2 / Q000235:genetics* / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D011956:Receptors, Cell Surface; D011993:Recombinant Fusion Proteins; D022641:Shiga Toxin 2; D007987:Gonadotropin-Releasing Hormone