[Indirect method ELISA for the detection of antibodies against bovine diarrhea virus in bovine serum].
Serological diagnosis is very important for the detection of bovine viral diarrhea virus, an important pathogen related to reproductive failure. Methods normally used for the detection of antibodies are serum neutralization (SN) and ELISA. The objective of this work was to standardize an indirect ELISA with SN. With a diagnostic sensitivity of 98.31% and a diagnostic specificity of 100%, this ELISA-BVDV shows good sensitivity, specificity and repeatability. It is easy to transfer, economical, and easy to perform.
Pacheco
J M
JM
Instituto de Virología, CICV-INTA, Castelar, CC. 77, 1708 Morón, Buenos Aires, Argentina. juanmanuelpacheco@hotmail.com
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D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000097:blood* / Q000175:diagnosis / Q000276:immunology; D002417:Cattle; D002460:Cell Line / Q000821:virology; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D009500:Neutralization Tests; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies
D000914:Antibodies, Viral