Differential effects of Matrigel and its components on functional activity of CFTR and ENaC in mouse endometrial epithelial cells.
Our previous studies have observed an effect of Matrigel, a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, on the expression of ion channels in mouse endometrial epithelia; namely the cystic fibrosis transmembrane conductance regulator (CFTR), a cAMP-dependent Cl(-)channel, and the epithelial sodium channel (ENaC). The present study further investigated the effects of Matrigel and its individual components on the functional expression of CFTR and ENaC using the short-circuit current (Isc) technique. The results showed that different components of Matrigel, namely growth factors, laminin and collagen, had differential effects on the functional activity of the two ion channels in murine endometrial epithelium. The information obtained may be useful for designing future in vitro culture models to investigate the functional roles of these ion channels in the endometrium.
Yang
Jian Zhi
JZ
Epithelial Cell Biology Research Center, Department of Physiology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, NT, HKSAR, Hong Kong.
Ho
Alice Lok Sze
AL
Ajonuma
Louis Chukwuemeka
LC
Lam
Sun Yee
SY
Tsang
Lai Ling
LL
Tang
Ning
N
Rowlands
Dewi Kenneth
DK
Gou
Yu Lin
YL
Chung
Yiu Wa
YW
Chan
Hsiao Chang
HC
D000818:Animals; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000378:metabolism*; D004338:Drug Combinations; D004717:Endometrium / Q000187:drug effects* / Q000378:metabolism; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D053503:Epithelial Sodium Channels; D005260:Female; D007797:Laminin / Q000494:pharmacology*; D051379:Mice; D008813:Mice, Inbred ICR; D011509:Proteoglycans / Q000494:pharmacology*; D015222:Sodium Channels / Q000378:metabolism*
D004338:Drug Combinations; D053503:Epithelial Sodium Channels; D007797:Laminin; D011509:Proteoglycans; D015222:Sodium Channels; C059485:matrigel; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D003094:Collagen