[Immunohistochemical research of integrin alpha 5 expression during mandibular fracture healing].
OBJECTIVE
To investigate the expression change of integrin alpha 5 during mandibular fracture healing.


METHODS
Using rabbit mandibular fracture model, the fractured bone tissues were obtained and paraffin slices were made on the days of 1, 3, 5, 7, 14, 30, 60 and 90 after surgery, respectively. Non-fractured mandibles were used as normal control. LsAB immunohistochemical method was used to detect the expression of integrin alpha 5 in bone tissue, especially on osteoblasts and osteoclasts.


RESULTS
Integrin alpha 5 was widely expressed in fractured bone and surrounding soft tissues. The expression of integrin alpha 5 increased 7 days after fracture, peaked in 14 to 30 days and returned to nearly normal in 60-90 days.


CONCLUSIONS
During mandibular fracture healing, the expression of integrin alpha 5 in bone tissue increased clearly. It is estimated that integrin alpha 5 players an important role in fracture healing.
Liu
Shaohua
S
Oral and Maxillofacial Department, Qilu Hospital of Shandong University, Jinan 250012, China.
Li
Shengwei
S
Cheng
Gang
G
D000818:Animals; D005260:Female; D017102:Fracture Healing; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D019012:Integrin beta1 / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D008337:Mandibular Fractures / Q000378:metabolism*; D010006:Osteoblasts / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011817:Rabbits
D019012:Integrin beta1; D012333:RNA, Messenger