Selection of peptides inhibiting a beta-lactamase-like activity.
A library of random peptide sequences was used to select peptides that inhibit an anti-idiotypic catalytic Ig, immunoglobulin (IgG) 9G4H9, with a beta-lactamase-like activity. This library displays cyclic heptapeptides on the surface of bacteriophages and represents a collection of up to 4.5 x 109 peptides. The first selection step aimed at enriching the library in species that bind to the whole Ig molecule. The second step was to discriminate peptides that bind to part of the molecule other than the active site. Selected peptides were then screened by surface plasmon resonance analysis. Those displaying measurable Kd values were assayed for their ability to inhibit the catalytic Ig.
Yribarren
Anne-Sophie
AS
Génie enzymatique et cellulaire, UMR 6022 CNRS, Université de Technologie de Compiègne, France.
Thomas
Daniel
D
Friboulet
Alain
A
Avalle
Bérangère
B
D000595:Amino Acid Sequence; D000818:Animals; D017151:Antibodies, Catalytic / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D007074:Immunoglobulin G / Q000378:metabolism*; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D011485:Protein Binding; D020349:Surface Plasmon Resonance; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases / Q000378:metabolism
D017151:Antibodies, Catalytic; D004791:Enzyme Inhibitors; D007074:Immunoglobulin G; D019151:Peptide Library; D010455:Peptides; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases