Characterization and formation mechanism of water-insoluble DNA-matrix induced by UV irradiation.
We have prepared water-insoluble and nuclease resistant DNA-matrixes by UV irradiation. The UV-irradiated DNA-matrix could effectively accumulate and condense harmful DNA-intercalating compounds, such as acridine orange (AO) and ethidium bromide (EB), from diluted aqueous solutions. The binding constant of AO and EB for UV-irradiated DNA were determined to be 1.0 (+/- 0.2) x 10(5) M-1 and 6.8 (+/- 0.3) x 10(4) M-1, respectively; values consisted with reported results for non-irradiated DNA. In addition, the agarose gel electrophoresis and AFM measurements indicate that DNA matrix forms an intermolecular cross-linking structure with the radical reaction. The UV-irradiated DNA-matrixes have potential uses as a biomaterial filter for the removal of harmful DNA intercalating compounds.
Yamada
M
M
Division of Bioscience, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo 060-0810, Japan.
Satoh
S
S
Nomizu
M
M
Ohkawa
K
K
Yamamoto
H
H
Nishi
N
N
D000165:Acridine Orange / Q000378:metabolism; D004247:DNA / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects* / Q000648:ultrastructure; D007364:Intercalating Agents / Q000378:metabolism*; D018625:Microscopy, Atomic Force; D014466:Ultraviolet Rays; D014867:Water / Q000737:chemistry
D007364:Intercalating Agents; D014867:Water; D004247:DNA; D000165:Acridine Orange