title: Effects of metal ions on activity of plasmin.
abstract: The effects of some metal ions on amidolytic and fibrinogenolytic activities of highly purified human plasmin were investigated in vitro. In the presence of Zn2+, Cu2+, Cd2+, and Au+ in the incubation mixture at the concentrations of 1 x 10(-5) - 1x 10(-3) M, the amidolytic plasmin activity was strongly inhibited, whereas Ca2+ and Mg2+ at the same concentrations were not effective. The analysis of the kinetic study has shown that Zn2+ or Cu2+ acts as mixed-type inhibitors of plasmin activity. The inhibition of amidolytic plasmin activity by Zn2+ and Cu2+ was reduced in the presence of EDTA, histidine, or albumin. Incubation of plasmin with Zn2+ or Cu2+ (at the concentration of 5 x 10(-4) M) resulted in complete loss of its proteolytic action on fibrinogen, whereas Cd2+ and Au+ under the same conditions only partially inhibited this process.
authors.affiliation: Department of General Biochemistry, University of Lodz, Lodz, Poland.
authors.forename: Pawel
authors.initials: P
authors.lastname: Nowak
authors.forename: Alojzy
authors.initials: A
authors.lastname: Zgirski
mesh_terms: D001798:Blood Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005341:Fibrinolysin / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006801:Humans; D007477:Ions / Q000494:pharmacology; D007700:Kinetics; D008670:Metals / Q000494:pharmacology*
chemical_list: D001798:Blood Proteins; D004791:Enzyme Inhibitors; D007477:Ions; D008670:Metals; D005341:Fibrinolysin