Sodium selenate partially corrects impaired functional responses in detrusor muscle in streptozotocin-induced diabetic rats.
Selenium selenate was administered to streptozotocin-induced diabetic rats to assess its effects on the detrusor muscle. Thirty-two rats were divided into four groups of eight subjects each. The study animals were made diabetic by means of a single intravenous injection of streptozotocin (STZ). The responsiveness of the detrusor was improved in the group injected with sodium selenate. Diabetes caused significant increases in carbachol and beta,gamma-MeATP-evoked contractions and significant decrease of contractions induced by electrical stimulation. Isoprenaline-induced relaxation of the detrusor muscle was diminished by diabetes, whereas ATP relaxation appeared to be increased. Although adenosine-induced relaxations in controls and in diabetic rats were accompanied by unchanged responses in normoxic conditions, a significant enhancement in the detrusor muscle was observed during hypoxia. This enhancement of adenosine responsiveness in hypoxic conditions is inhibited in diabetes. Treatment with sodium selenate prevented alterations of both carbachol-induced contractility and isoprenaline-evoked relaxation, whereas nerve-mediated contractions and purinergic responses were not improved in diabetic rats after treatment. Our data suggest that changes in cholinergic and adrenergic responses were the result of selenium deficiency in diabetic rats.
Gür
Serap
S
Department of Pharmacology, Faculty of Pharmacy, Ankara University, 06100 Ankara, Turkey.
Cinel
Ismail
I
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000139:chemically induced / Q000503:physiopathology*; D066298:In Vitro Techniques; D007274:Injections, Intraperitoneal; D023421:Models, Animal; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects* / Q000502:physiology*; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D064586:Selenic Acid; D012643:Selenium / Q000097:blood / Q000172:deficiency; D018036:Selenium Compounds / Q000097:blood / Q000494:pharmacology*; D013311:Streptozocin; D001743:Urinary Bladder / Q000187:drug effects* / Q000502:physiology*
D018036:Selenium Compounds; D013311:Streptozocin; D012643:Selenium; D064586:Selenic Acid