title: Expression of claudin-5 in dermal vascular endothelia.
abstract: Claudins and occludin are integral membrane proteins at tight junctions (TJs). We examined subcellular localization of claudin-5 and occludin in dermal vascular endothelia. Immunofluorescence staining showed that claudin-5 was expressed at the cell-cell border of dermal vascular endothelia in mouse skin. However, in some dermal vessels, claudin-5 expression was markedly decreased or absent in amount by double-immunofluorescence stainings with PECAM-1 and PAL-E. In contrast, occludin was not detected in dermal vessels. Freeze-fracture and immunoreplica electron microscopy on primary-cultured human dermal endothelial cells showed that claudin-5 was localized at tight junctions. These findings confirmed that TJs in dermal vascular endothelial cells are composed of claudin-5.
authors.affiliation: Department of Dermatology, Graduate School of Medicine, Kyoto University, Kyoto, Japan. kamorita@kuhp.kyoto-u.ac.jp
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Morita
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sasaki
authors.forename: Kyoko
authors.initials: K
authors.lastname: Furuse
authors.forename: Mikio
authors.initials: M
authors.lastname: Furuse
authors.forename: Shoichiro
authors.initials: S
authors.lastname: Tsukita
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Miyachi
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D062507:Claudin-5; D020405:Dermis / Q000098:blood supply*; D004730:Endothelium, Vascular / Q000502:physiology* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D005614:Freeze Fracturing; D015870:Gene Expression; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D062793:Occludin; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism
chemical_list: D062507:Claudin-5; C491511:Cldn5 protein, mouse; D008565:Membrane Proteins; C568985:OCLN protein, human; D062793:Occludin; C568986:Ocln protein, mouse; D019408:Platelet Endothelial Cell Adhesion Molecule-1