Evaluation of fungal burden in experimental paracoccidioidomycosis by using the fluorescent dye Blankophor.
The fungal load in organs and blood of susceptible and resistant mice infected with Paracoccidioides brasiliensis was quantitated by using the optical brightener Blankophor and compared with CFU counts. Fluorescent staining of fungal cells proved to be a quick and easy procedure, suitable for evaluation of paracoccidioidomycotic infection.
Nishikaku
Angela Satie
AS
Departamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brazil.
Burger
Eva
E
D000818:Animals; D001557:Benzenesulfonates / Q000378:metabolism*; D001769:Blood / Q000382:microbiology; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D005456:Fluorescent Dyes / Q000378:metabolism*; D016469:Fungemia / Q000382:microbiology; D006801:Humans; D051379:Mice; D009852:Omentum / Q000382:microbiology; D010179:Pancreas / Q000382:microbiology; D010228:Paracoccidioides / Q000302:isolation & purification* / Q000472:pathogenicity; D010229:Paracoccidioidomycosis / Q000382:microbiology* / Q000503:physiopathology; D013154:Spleen / Q000382:microbiology; D014930:Women
D001557:Benzenesulfonates; D005456:Fluorescent Dyes; C414926:Blankophore P fluessig