title: Expression and distribution of basement membrane proteins in rat larynx and trachea following irradiation.
abstract: OBJECTIVE
  Basement membranes-(BM) influence polarization, differentiation, migration and proliferation of cell and play an important role in maintaining structural and functional tissue integrity. While BM alterations have been reported in various lesions (e.g. inflammation, tumors) of laryngeal-tracheal tissues, reports on radiogenic BM alterations are rare. External irradiation (IRR) of advanced head and neck tumors often includes "normal tissues" (tissues without cancer) of the larynx. In these normal tissues both single-cell damage (necrosis, apoptosis, functional cell death) and interstitial damage (edema, fibrosis, vascular alterations, cellular infiltrations) resulting in tissue remodeling can occur, depending on various IRR parameters. In this study, we set out to add to our knowledge on the phenotypic characterization of the radiogenic BM expression pattern in laryngo-tracheal tissues.
  
  
  METHODS
  In 63 laryngo-tracheal specimens from Wistar rats, we investigated the laminin (LA) and collagen IV (CIV) expression profile and distribution pattern depending on the IRR dose (fractionated IRR, 2 Gy/day, up to a total dose of 20, 40, or 60 Gy), the time since IRR (6 months vs 12 months) and animal age (1 year vs 1.5 years) using immunohistochemical methods, semiquantitative assessment, and multivariate analysis.
  
  
  RESULTS
  In specimens irradiated with more than 20 Gy, both BM constituents predominantly showed dose-dependent increases and sometimes fluctuations in staining at slight to moderate levels. The expression differed in frequency and level among the various tissue structures. In some structures there was decreased expression. In the vocalis muscle, laryngeal and esophageal nerve endings, recurrent laryngeal nerve and laryngeal and tracheal muscles, LA was detected at levels significantly stronger than in controls. BM surrounding gland structures, nerve endings of the piriform sinus and esophageal muscles showed a marked tendency towards increased LA expression. However, the BM underlying the mucosal layer of the supra- and subglottic region revealed decreasing LA immunoreaction up to 40 Gy IRR, but a distinct increase in expression at 60 Gy. In the esophageal and tracheal muscles, tracheal perichondrium, recurrent laryngeal nerve and capillaries, CIV was detected at significantly stronger levels than in the controls. The vocal ligament exhibited positive CIV immunoreactions adjacent to interstitial and infiltrate cells and CIV-positive BM condensations, resulting in increased staining of these structures. CIV reactions of laryngeal and hypopharyngeal nerve endings tended towards increased expression. In contrast, BM staining surrounding vocal muscle cells revealed significantly decreased expression. In addition, there was a tendency towards decreased expression for supraglottic, subglottic and hypopharyngeal muscle cells. Age and time since irradiation had no significant effect on staining.
  
  
  CONCLUSIONS
  The BM constituents laminin and collagen IV showed prominent dose-dependent increases and sometimes fluctuations in expression. This expression pattern persisted up to one year after completion of the irradiation. Thus, these findings must be related to late radiation effects. The altered BM expression may play a role, at least in part, in structural (e.g. laryngeal edema) and functional (voice disorders) changes associated with irradiation of the head and neck area.
authors.affiliation: Department of Otorhinolaryngology, University Hospital, Martin-Luther University Halle-Wittenberg, Magdeburger Strasse 12, 06097 Halle a.d. Saale, Germany. sylva.bartel-friedrich@meditin.uni-halle.de
authors.forename: S
authors.initials: S
authors.lastname: Bartel-Friedrich
authors.forename: R E
authors.initials: RE
authors.lastname: Friedrich
authors.forename: C
authors.initials: C
authors.lastname: Lautenschläger
authors.forename: H J
authors.initials: HJ
authors.lastname: Holzhausen
authors.forename: K
authors.initials: K
authors.lastname: Röser
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000378:metabolism / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D016326:Extracellular Matrix Proteins / Q000378:metabolism* / Q000528:radiation effects; D007830:Larynx / Q000378:metabolism / Q000528:radiation effects*; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D013997:Time Factors; D014132:Trachea / Q000378:metabolism / Q000528:radiation effects*
chemical_list: D016326:Extracellular Matrix Proteins