title: Studies on the electrochemical performance of glucose biosensor based on ferrocene encapsulated ORMOSIL and glucose oxidase modified graphite paste electrode.
abstract: The electrochemical performance of a new glucose biosensor is reported. The glucose biosensor is developed using glucose oxidase (GOD) and ferrocene encapsulated palladium (Pd)-linked organically modified sol-gel glass (ORMOSIL) material incorporated within graphite paste electrode. The ORMOSIL material incorporated within graphite paste electrode behaves as an excellent electrocatalyst for the oxidation of enzymatically reduced GOD. The electrochemical behavior of new glucose biosensor has been examined by cyclic volammetry and amperometric measurements. The bioelectrocatalysis of ORMOSIL embedded within graphite paste as a function of storage time and varying concentration of ORMOSIL is reported. The initial amperometric response on glucose sensing is recorded to be 145 microA at 15% (w/w) concentration of the ORMOSIL which is decreased to 20 microA at 5% of the same keeping GOD concentration constant. The variation of electrochemical behavior of the ORMOSIL embedded within graphite paste as a function of time has also been studied based on cyclic voltammetry. The voltammograms showing the reversible electrochemistry of ORMOSIL encapsulated ferrocene is changed into a plateau shape as a function of time, however, the electrocatalytic behavior is still retained. The practical usability of new glucose sensor has been compared with earlier developed glucose sensor. The sensitivity, response time and linearity of the new glucose biosensor are found to be excellent over earlier reported glucose biosensor. The amperometric response, calibration curve and practical applications of new glucose sensor are reported.
authors.affiliation: Department of Chemistry, Banaras Hindu University, Varanasi 221005, India. p_c_pandey@satyam.net.in
authors.forename: P C
authors.initials: PC
authors.lastname: Pandey
authors.forename: S
authors.initials: S
authors.lastname: Upadhyay
authors.forename: N K
authors.initials: NK
authors.lastname: Shukla
authors.forename: S
authors.initials: S
authors.lastname: Sharma
mesh_terms: D015374:Biosensing Techniques; D004566:Electrodes; D005296:Ferrous Compounds; D005898:Glass; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000378:metabolism*; D006108:Graphite; D000075163:Metallocenes; D010165:Palladium
chemical_list: D005296:Ferrous Compounds; D000075163:Metallocenes; D010165:Palladium; D006108:Graphite; D005949:Glucose Oxidase; D005947:Glucose; C004998:ferrocene