title: Basal and inducible levels of Hsp70 in patients with acute heat illness induced during training.
abstract: Heat shock proteins (Hsps) or stress proteins, and, in particular, the inducible, cytosolic Hsp70, represent a highly conserved response to heat exposure and to a variety of noxious stimuli. Many investigations have shown correlations between the aberrant expression of Hsps and disease states. Whether the basal and inducible levels of Hsp70 are of any biological significance in patients with heat-induced diseases remains unknown. In the present study, we compared the basal and inducible levels of Hsp70 by flow cytometry in lymphocytes of patients with heat-induced diseases and after recovery from this disease, and in matched controls. Both groups comprised individuals who exercised by running in the same hot environment. The level of inducible Hsp70 was also measured after a heat treatment of lymphocytes in vitro. The results show that there is variation of basal and inducible Hsp70 levels among individuals. However, the group of patients suffering from heat-induced illnesses in May shows a significantly higher basal (P = 0.02) level of Hsp70 than does the control group. Individuals who have an increased level of Hsp70 may be more sensitive to heat or may respond differently. The level of Hsp70 may represent a biomarker to evaluate whether they are more susceptible to stresses than other individuals. Interestingly, the basal level of Hsp70 is higher in both the patient group and the control group in November than in May. In fact, the basal levels of Hsp70 in the patient and control groups are essentially the same in November, perhaps reflecting the successful stress conditioning of both groups.
authors.affiliation: Institute of Occupational Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, People's Republic of China.
authors.forename: Chengfeng
authors.initials: C
authors.lastname: Xiao
authors.forename: Tangchun
authors.initials: T
authors.lastname: Wu
authors.forename: Aiming
authors.initials: A
authors.lastname: Ren
authors.forename: Qin
authors.initials: Q
authors.lastname: Pan
authors.forename: Sheng
authors.initials: S
authors.lastname: Chen
authors.forename: Fen
authors.initials: F
authors.lastname: Wu
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Li
authors.forename: Ruibo
authors.initials: R
authors.lastname: Wang
authors.forename: Lawrence E
authors.initials: LE
authors.lastname: Hightower
authors.forename: Robert M
authors.initials: RM
authors.lastname: Tanguay
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D002681:China; D015444:Exercise; D005434:Flow Cytometry; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*; D018882:Heat Stress Disorders / Q000378:metabolism*; D006358:Hot Temperature / Q000009:adverse effects; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000378:metabolism; D008297:Male
chemical_list: D018840:HSP70 Heat-Shock Proteins