title: Physical and transcript map of the minimally deleted region III on 17p implicated in the early development of Barrett's oesophageal adenocarcinoma.
abstract: Allelic imbalance (AI) studies on chromosome 17 (C17) in Barrett's oesophageal adenocarcinoma (BOA) tumours strongly suggest that a minimally deleted region on C17p harbours a BOA-associated gene with tumour suppressor function. This deleted region, designated minimal region III (MRIII), lies between the two microsatellite markers D17S1852 and D17S954. Computational sequence analysis techniques, BLAST and NIX, were used to assemble a physical map of MRIII, consisting of three overlapping bacterial artificial chromosome (BAC) clones, 297N7, 963H4 and 795F17, from the RPCI-11 library. The 270 kb genomic sequence of MRIII was analysed using the computational gene prediction methods NIX and TAP to identify putative BOA genes. A transcript map of MRIII has been generated and contains 25 candidate BOA genes, four of which are the named genes MYH3, SCO1, x006 and MAGOH-LIKE. The other candidates consist of seven genes predicted by TAP with associated ESTs identified by NIX, two genes predicted by TAP alone and 12 genes/ESTs (or pairs of ESTs) identified by NIX alone. No disease-specific mutations were identified in x006 or MAGOH-LIKE, although expression analysis of these genes suggests that they may show alternative splicing or be altered epigenetically or in regulatory regions in oesophageal cancer.
authors.affiliation: Molecular Genetics and Oncology Group, Clinical Dental Sciences, The University of Liverpool, Liverpool L69 3BX, UK.
authors.forename: Julie R
authors.initials: JR
authors.lastname: Dunn
authors.forename: Janet M
authors.initials: JM
authors.lastname: Risk
authors.forename: Joanne E
authors.initials: JE
authors.lastname: Langan
authors.forename: Damian
authors.initials: D
authors.lastname: Marlee
authors.forename: Anthony
authors.initials: A
authors.lastname: Ellis
authors.forename: Fiona
authors.initials: F
authors.lastname: Campbell
authors.forename: Alastair J M
authors.initials: AJ
authors.lastname: Watson
authors.forename: John K
authors.initials: JK
authors.lastname: Field
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics*; D001471:Barrett Esophagus / Q000235:genetics*; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17; D003001:Cloning, Molecular; D004938:Esophageal Neoplasms / Q000235:genetics*; D020224:Expressed Sequence Tags; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics; D008957:Models, Genetic; D009154:Mutation; D020161:Physical Chromosome Mapping; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012984:Software; D014407:Tumor Cells, Cultured
chemical_list: D012333:RNA, Messenger