In situ Ca2+ imaging of odor responses in a coronal olfactory epithelium slice.
An in situ Ca2+ -imaging technique was adopted to monitor odorant responses of more than several hundreds of neurons simultaneously in an intact coronal slice of the mouse olfactory epithelium. The sensitivity and resolution of the slice Ca2+ -imaging were high enough to distinguish between olfactory receptor neurons with threshold concentrations in a one-order difference for a particular odorant at the single-cell level. Increasing odorant concentrations resulted in increases in the numbers of odorant-responsive neurons, which were visualized in situ in the coronal slice. The methodology established in this study is a powerful tool to visualize spatial distributions of odorant responsive neurons at a cellular resolution, and to construct odor maps in a coronal view of the olfactory epithelium.
Omura
Masayo
M
Department of Integrated Biosciences, University of Tokyo, Chiba 277-8562, Japan.
Sekine
Hisaki
H
Shimizu
Takao
T
Kataoka
Hiroshi
H
Touhara
Kazushige
K
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000779:Anesthetics, Local / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D019905:Benzyl Alcohol / Q000494:pharmacology; D002118:Calcium / Q000502:physiology*; D005576:Colforsin / Q000494:pharmacology; D003952:Diagnostic Imaging / Q000379:methods; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004533:Egtazic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D016257:Fura-2 / Q000031:analogs & derivatives* / Q000378:metabolism; D019850:Heptanol / Q000494:pharmacology; D066298:In Vitro Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods; D009812:Odorants; D009831:Olfactory Mucosa / Q000187:drug effects / Q000502:physiology*; D018034:Olfactory Receptor Neurons / Q000187:drug effects / Q000502:physiology*; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D013268:Stimulation, Chemical
D000779:Anesthetics, Local; D010726:Phosphodiesterase Inhibitors; C098106:fura-PE3; C070379:1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester; D005576:Colforsin; D004533:Egtazic Acid; D019850:Heptanol; D019905:Benzyl Alcohol; D011188:Potassium; D002118:Calcium; D015056:1-Methyl-3-isobutylxanthine; D016257:Fura-2