Colorimetric dehydrogenase screen based on NAD(P)H generation.
Mayer
Kimberly M
KM
Biology Department, Brookhaven National Laboratory, Upton, NY, USA.
D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D003124:Colorimetry / Q000379:methods; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D007202:Indicators and Reagents; D007700:Kinetics; D009243:NAD / Q000032:analysis*; D009249:NADP / Q000032:analysis*; D010088:Oxidoreductases / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D010734:Phosphogluconate Dehydrogenase / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000032:analysis / Q000378:metabolism; D015183:Restriction Mapping
D007202:Indicators and Reagents; D011994:Recombinant Proteins; D009243:NAD; D009249:NADP; D010088:Oxidoreductases; D010734:Phosphogluconate Dehydrogenase