Rhizoremediation of dioxin-like compounds by a recombinant Rhizobium tropici strain expressing carbazole 1,9a-dioxygenase constitutively.
A recombinant Rhizobium strain, PBK3-IS, that constitutively expressed the oxygenase component of carbazole 1,9a-dioxygenase from Sphingomonas sp. strain KA1, was constructed. In the water-cultured siratro rhizospheres inoculated with strain PBK3-IS, 48% of the dibenzofuran was removed within 3 days (initial substrate, 25 microg). Similar results were obtained in soil-cultured siratro rhizospheres using sterile vermiculite. When non-sterile field soils were used instead of sterile vermiculite, the inoculated recombinant strain could grow on the siratro root in all soils tested, except for wet paddy field.
Saiki
Yuko
Y
Fundamental Research Laboratory, Asahi Breweries Ltd., 1-21 Midori 1-chome, Moriya-shi, Ibaraki 302-0106, Japan.
Habe
Hiroshi
H
Yuuki
Toshifumi
T
Ikeda
Mitsuo
M
Yoshida
Takako
T
Nojiri
Hideaki
H
Omori
Toshio
T
D001426:Bacterial Proteins; D001673:Biodegradation, Environmental; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000235:genetics; D004147:Dioxins / Q000378:metabolism*; D049308:Dioxygenases; D004785:Environmental Pollutants / Q000378:metabolism*; D007887:Fabaceae / Q000378:metabolism; D010105:Oxygenases / Q000096:biosynthesis* / Q000235:genetics; D018517:Plant Roots / Q000382:microbiology; D010957:Plasmids / Q000235:genetics; D012231:Rhizobium / Q000201:enzymology* / Q000235:genetics; D012988:Soil Microbiology; D020578:Sphingomonas / Q000201:enzymology / Q000235:genetics
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004147:Dioxins; D004785:Environmental Pollutants; D010105:Oxygenases; D049308:Dioxygenases; C108434:carbazole 1,9a-dioxygenase