Observation of multiple isoforms and specific proteolysis patterns of proliferating cell nuclear antigen in the context of cell cycle compartments and sample preparations.
The proliferating cell nuclear antigen (PCNA) is an essential component for eukaryotic chromosomal DNA replication and repair. PCNA forms a homotrimer ring, which may function as a DNA sliding clamp for DNA polymerases and, possibly, a docking station for other replication- and repair-related proteins. Several reports have suggested the existence of different PCNA isoforms. Here we confirm, using high resolution two-dimensional electrophoresis with narrow pH ranges, the existence of three PCNA isoforms in both Chinese hamster and human breast cancer cells. Among the three isoforms, M or main form is the dominant one throughout the cell cycle while the relative amounts of the minor components A (acidic) and B (basic) forms appear to vary during the cell cycle. We also observed that a specific pattern of PCNA proteolysis occurred during isoelectric focusing in spite of high urea (8 M) and detergent (2% 3-[(3-cholamidopropyl)dimethylamino]-1-propane sulfonate), which was largely inhibited by the proteosome inhibitor MG132 or boiling. Interestingly, the proteolysis pattern was mainly observed with samples isolated from cells in S and G2 phases. A similar but much lower level of PCNA proteolysis also occurred in vivo within the nuclei of the cells in S phase. Taken together, our data are consistent with the idea that the existence of the different isoforms and specific proteolysis of PCNA are relevant to its functions in vivo.
Naryzhny
Stanislav N
SN
Northeastern Ontario Regional Cancer Centre, Ontario, Canada.
Lee
Hoyun
H
D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D001943:Breast Neoplasms / Q000378:metabolism*; D016466:CHO Cells; D002453:Cell Cycle; D045744:Cell Line, Tumor; D006224:Cricetinae; D003412:Cricetulus; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005720:Gamma Rays / Q000009:adverse effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism* / Q000528:radiation effects; D020033:Protein Isoforms / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism* / Q000528:radiation effects
D018809:Proliferating Cell Nuclear Antigen; D020033:Protein Isoforms