title: [Residue analysis of spectinomycin in tissues of chicken and swine by HPLC].
abstract: A reversed-phase HPLC method with ultraviolet detection using p-nitrophenyl hydrazine as a pre-column derivatizing reagent was investigated for the determination of the antibiotic spectinomycin (SPCM) in muscle, liver, kidney and fat of chicken and swine. SPCM was extracted from samples with 10% trichloroacetic acid saturated with EDTA-2Na, and then cleaned up with coupled Sep-Pak Plus PS-2 cartridges. The detection limit of SPCM was 0.02 microgram (potency)/g. Recoveries of SPCM ranged from 77.4 to 97.4% for chicken tissues and from 74.5 to 91.8% for swine tissues. The present method was used for the analysis of chicken tissues after the 11th day of withdrawal (SPCM-medicated drinking water: 500 mg (potency)/L, for 7 days), and swine tissues after the 14th day of withdrawal (SPCM-medicated feed: 100 mg (potency)/kg, for 7 days). Results showed that SPCM concentrations were lower than the MRLs in all tissues.
authors.affiliation: National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries: 1-15-1, Tokura, Kokubunji, Tokyo 185-8511, Japan.
authors.forename: Kouko
authors.initials: K
authors.lastname: Hamamoto
authors.forename: Yasuharu
authors.initials: Y
authors.lastname: Mizuno
authors.forename: Ryoji
authors.initials: R
authors.lastname: Koike
authors.forename: Ryozo
authors.initials: R
authors.lastname: Yamaoka
authors.forename: Toshio
authors.initials: T
authors.lastname: Takahashi
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Takahashi
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002645:Chickens / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004350:Drug Residues / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D000198:Spectinomycin / Q000032:analysis*; D013552:Swine / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D000198:Spectinomycin