title: Transcriptional profile of the human peripheral nervous system by serial analysis of gene expression.
abstract: The peripheral nerve contains both nonmyelinating and myelinating Schwann cells. The interactions between axons, surrounding myelin, and Schwann cells are thought to be important for the correct functioning of the nervous system. To get insight into the genes involved in human myelination and maintenance of the myelin sheath and nerve, we performed a serial analysis of gene expression of human sciatic nerve and cultured Schwann cells. In the sciatic nerve library, we found high expression of genes encoding proteins related to lipid metabolism, the complement system, and the cell cycle, while cultured Schwann cells showed mainly high expression of genes encoding extracellular matrix proteins. The results of our study will assist in the identification of genes involved in maintenance of myelin and peripheral nerve and of genes involved in inherited peripheral neuropathies.
authors.affiliation: Department of Neurogenetics, Academic Medical Center, University of Amsterdam, 1100 DD Amsterdam, The Netherlands.
authors.forename: Rosalein R
authors.initials: RR
authors.lastname: de Jonge
authors.forename: Jeroen P
authors.initials: JP
authors.lastname: Vreijling
authors.forename: Asker
authors.initials: A
authors.lastname: Meintjes
authors.forename: Marcel S G
authors.initials: MS
authors.lastname: Kwa
authors.forename: A H C
authors.initials: AH
authors.lastname: van Kampen
authors.forename: Ivo N
authors.initials: IN
authors.lastname: van Schaik
authors.forename: Frank
authors.initials: F
authors.lastname: Baas
mesh_terms: D015152:Blotting, Northern; D002478:Cells, Cultured; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012583:Schwann Cells / Q000502:physiology*; D012584:Sciatic Nerve / Q000502:physiology*