title: A panoramic view of yeast noncoding RNA processing.
abstract: Predictive analysis using publicly available yeast functional genomics and proteomics data suggests that many more proteins may be involved in biogenesis of ribonucleoproteins than are currently known. Using a microarray that monitors abundance and processing of noncoding RNAs, we analyzed 468 yeast strains carrying mutations in protein-coding genes, most of which have not previously been associated with RNA or RNP synthesis. Many strains mutated in uncharacterized genes displayed aberrant noncoding RNA profiles. Ten factors involved in noncoding RNA biogenesis were verified by further experimentation, including a protein required for 20S pre-rRNA processing (Tsr2p), a protein associated with the nuclear exosome (Lrp1p), and a factor required for box C/D snoRNA accumulation (Bcd1p). These data present a global view of yeast noncoding RNA processing and confirm that many currently uncharacterized yeast proteins are involved in biogenesis of noncoding RNA.
authors.affiliation: Banting and Best Department of Medical Research, University of Toronto, 112 College Street, M5G 1L6, Toronto, Ontario, Canada.
authors.forename: Wen Tao
authors.initials: WT
authors.lastname: Peng
authors.forename: Mark D
authors.initials: MD
authors.lastname: Robinson
authors.forename: Sanie
authors.initials: S
authors.lastname: Mnaimneh
authors.forename: Nevan J
authors.initials: NJ
authors.lastname: Krogan
authors.forename: Gerard
authors.initials: G
authors.lastname: Cagney
authors.forename: Quaid
authors.initials: Q
authors.lastname: Morris
authors.forename: Armaity P
authors.initials: AP
authors.lastname: Davierwala
authors.forename: Jörg
authors.initials: J
authors.lastname: Grigull
authors.forename: Xueqi
authors.initials: X
authors.lastname: Yang
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.forename: Nicholas
authors.initials: N
authors.lastname: Mitsakakis
authors.forename: Owen W
authors.initials: OW
authors.lastname: Ryan
authors.forename: Nira
authors.initials: N
authors.lastname: Datta
authors.forename: Vladimir
authors.initials: V
authors.lastname: Jojic
authors.forename: Chris
authors.initials: C
authors.lastname: Pal
authors.forename: Veronica
authors.initials: V
authors.lastname: Canadien
authors.forename: Dawn
authors.initials: D
authors.lastname: Richards
authors.forename: Bryan
authors.initials: B
authors.lastname: Beattie
authors.forename: Lani F
authors.initials: LF
authors.lastname: Wu
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Altschuler
authors.forename: Sam
authors.initials: S
authors.lastname: Roweis
authors.forename: Brendan J
authors.initials: BJ
authors.lastname: Frey
authors.forename: Andrew
authors.initials: A
authors.lastname: Emili
authors.forename: Jack F
authors.initials: JF
authors.lastname: Greenblatt
authors.forename: Timothy R
authors.initials: TR
authors.lastname: Hughes
mesh_terms: D002478:Cells, Cultured; D005656:Fungal Proteins / Q000235:genetics / Q000302:isolation & purification; D015966:Gene Expression Regulation, Fungal / Q000235:genetics*; D016681:Genome, Fungal; D009154:Mutation / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D012322:RNA Precursors / Q000096:biosynthesis / Q000235:genetics; D020537:RNA, Small Nucleolar / Q000096:biosynthesis / Q000235:genetics; D012343:RNA, Transfer / Q000096:biosynthesis / Q000235:genetics; D022661:RNA, Untranslated / Q000096:biosynthesis* / Q000235:genetics; D012261:Ribonucleoproteins / Q000096:biosynthesis*; D015003:Yeasts / Q000235:genetics / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D012322:RNA Precursors; D020537:RNA, Small Nucleolar; D022661:RNA, Untranslated; D012261:Ribonucleoproteins; D012343:RNA, Transfer