Insulin-like growth factor-I and its binding proteins in colostrum compared to measures in serum of Holstein neonates.
Colostral insulin-like growth factor-I (IGF-I) may be beneficial in the development of gastrointestinal tracts of bovine neonates. Thus, the purpose of this study was to examine relationships among concentrations of IGF-I and IGF-binding proteins (IGFBP) in colostrum used at two initial feedings and serum concentrations of IGF-I, IGFBP, total protein, gamma glutamyltransferase (GGT), and immunoglobulin G at 0 and 48 h after birth in Holstein neonates. Calves (n = 22) were separated from dams immediately after birth. Blood samples were taken before initial feeding and at 48 h after birth. Calves were fed 2 L of colostrum twice and milk replacer thereafter. Linear regression of serum IGF-I at 48 h and colostral IGF-I revealed a significant positive relationship (R2 = 0.204). Serum IGFBP-3 at 48 h and colostral IGFBP-3 also had a positive relationship (R2 = 0.143). However, linear regression of colostral IGF-I on the difference in serum IGF-I at 48 and 0 h was not significant. Calves were assigned to group 1 (0-h serum IGF-I < 10 ng/ml; n = 11) or group 2 (0-h serum IGF-I > or = 10 ng/ml; n = 11) for further analysis. There were no differences in serum IGF-I or IGFBP-2, -3, -4, and -5 concentrations at 48 h between groups 1 and 2. Correlation coefficients revealed negative relationships of serum IGF-I at 0 h to the difference between serum IGF-I at 48 and 0 h (r = -0.824), as well as birth weight of the calf to the amount of GGT at 48 h (r = -0.604). Females had lower birth weights than males, but sex of calf did not affect serum measures. At 0 h, but not 48 h, total serum protein was correlated to serum GGT concentrations (r = 0.573). From indirect evidence, absorption of colostral IGF-I and IGFBP-3 into systemic circulation may occur, but relative importance compared to endogenous sources is uncertain.
Sparks
A L
AL
Department of Animal Science, Oklahoma State University, Stillwater, OK 74078, USA.
Kirkpatrick
J G
JG
Chamberlain
C S
CS
Waldner
D
D
Spicer
L J
LJ
D000818:Animals; D000831:Animals, Newborn / Q000097:blood*; D002417:Cattle / Q000097:blood / Q000378:metabolism*; D003126:Colostrum / Q000737:chemistry*; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000032:analysis / Q000097:blood; D018974:Insulin-Like Growth Factor Binding Protein 4 / Q000032:analysis / Q000097:blood; D018975:Insulin-Like Growth Factor Binding Protein 5 / Q000032:analysis / Q000097:blood; D018969:Insulin-Like Growth Factor Binding Proteins / Q000032:analysis* / Q000097:blood; D007334:Insulin-Like Growth Factor I / Q000032:analysis*; D016014:Linear Models; D008297:Male; D012727:Sex Characteristics; D013997:Time Factors; D005723:gamma-Glutamyltransferase / Q000097:blood
D007074:Immunoglobulin G; D018972:Insulin-Like Growth Factor Binding Protein 3; D018974:Insulin-Like Growth Factor Binding Protein 4; D018975:Insulin-Like Growth Factor Binding Protein 5; D018969:Insulin-Like Growth Factor Binding Proteins; D007334:Insulin-Like Growth Factor I; D005723:gamma-Glutamyltransferase