Crystallization and preliminary X-ray analysis of Yersinia pseudotuberculosis-derived mitogen.
Yersinia pseudotuberculosis-derived mitogen (YPM), a superantigen with no amino-acid sequence similarity to other known superantigens, has been crystallized by the sitting-drop vapour-diffusion method. The crystals belong to space group C2, with unit-cell parameters a = 138.67, b = 78.66, c = 32.91 A, beta = 91.97 degrees. A native data set has been collected to a resolution of 1.8 A using synchrotron radiation. Self-rotation function calculations suggest the presence of three molecules in the asymmetric unit, corresponding to a solvent content of 45%.
Donadini
Roberta
R
School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia.
Wåhlberg
Malin
M
Kohsaka
Takao
T
Ito
Yasuhiko
Y
Fields
Barry A
BA
D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D003001:Cloning, Molecular; D003460:Crystallization; D004926:Escherichia coli / Q000235:genetics; D008934:Mitogens / Q000737:chemistry*; D011993:Recombinant Fusion Proteins; D012645:Selenomethionine; D018089:Superantigens / Q000737:chemistry; D014961:X-Ray Diffraction; D015011:Yersinia pseudotuberculosis / Q000737:chemistry
D001426:Bacterial Proteins; D008934:Mitogens; D011993:Recombinant Fusion Proteins; D018089:Superantigens; C093313:Yersinia pseudotuberculosis-derived mitogen; D012645:Selenomethionine