Effect of antibodies to intercellular adhesion molecule (ICAM)-1 and lymphocyte function-associated antigen (LFA)-1 on cytokine mRNA expression in reovirus type-2-triggered autoimmune insulitis in suckling DBA/1 mice.
Interleukin (IL)-12 in the presence of IL-18 induces T helper (Th) 1 dominated systemic immune reactions, such as those that occur in autoimmune insulitis in suckling mice infected with reovirus type-2 (Reo-2). The role of intercellular adhesion molecule (ICAM)-1 and lymphocyte function-associated antigen (LFA)-1 on the induction of cytokines responsible for Reo-2-triggered insulitis with impaired glucose tolerance (IGT) was examined. One-day-old DBA/1 mice infected with Reo-2 intraperitoneally (i.p.) were treated i.p. with monoclonal antibodies (mAbs) against ICAM-1 and LFA-1 (ICAM-1/LFA-1) at 5 days post-infection (d.p.i.). At 10 d.p.i., the administration of the mAbs was seen to have prevented the development of insulitis with IGT. This was associated with a decrease in the mRNA expression for IL-12(p40) and IL-18 by splenic cells. The present study suggests that ICAM-1/LFA-1 may be required for the differentiation of Th0 cells to Th1 cells, which mediate insulitis with IGT in Reo-2-infected suckling mice.
Hayashi
T
T
Laboratory of Veterinary Pathology, Faculty of Agriculture, Yamaguchi University, Yoshida, Japan.
Hasegawa
K
K
Morimoto
M
M
Onodera
T
T
D000818:Animals; D000831:Animals, Newborn; D000833:Animals, Suckling; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000627:therapeutic use*; D001327:Autoimmune Diseases / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control; D003921:Diabetes Mellitus, Experimental / Q000209:etiology / Q000378:metabolism*; D003922:Diabetes Mellitus, Type 1 / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control; D005260:Female; D005951:Glucose Tolerance Test; D007274:Injections, Intraperitoneal; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology*; D018664:Interleukin-12 / Q000096:biosynthesis* / Q000235:genetics; D053762:Interleukin-12 Subunit p40; D020382:Interleukin-18 / Q000096:biosynthesis* / Q000235:genetics; D007515:Islets of Langerhans / Q000276:immunology / Q000473:pathology; D016169:Lymphocyte Function-Associated Antigen-1 / Q000276:immunology*; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D030102:Orthoreovirus, Mammalian; D021122:Protein Subunits / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D012088:Reoviridae Infections / Q000150:complications / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D053762:Interleukin-12 Subunit p40; D020382:Interleukin-18; D016169:Lymphocyte Function-Associated Antigen-1; D021122:Protein Subunits; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1; D018664:Interleukin-12