A new coupling method for the synthesis of oligodeoxyribonucleotides based on protection by protonation of nucleoside bases.
We developed a conceptually new strategy for the coupling method in the phosphoramidite method without protection of nucleoside bases. This strategy, called "proton-block method", significantly reduced side reactions compared with the previous methods.
Ohkubo
A
A
Department of Life Science, Tokyo Institute of Technology, Nagatsuta, Midoriku, Yokohama 226-8501, Japan.
Seio
K
K
Sekine
M
M
D000241:Adenosine / Q000737:chemistry; D002625:Chemistry, Organic / Q000379:methods*; D003562:Cytidine / Q000737:chemistry; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000737:chemistry; D011522:Protons
D009838:Oligodeoxyribonucleotides; D009943:Organophosphorus Compounds; D011522:Protons; C434331:phosphoramidite; D003562:Cytidine; D000241:Adenosine