Molecular phylogenetic exploration of bacterial diversity in a Bakreshwar (India) hot spring and culture of Shewanella-related thermophiles.
The bacterial diversity of a hot spring in Bakreshwar, India, was investigated by a culture-independent approach. 16S ribosomal DNA clones derived from the sediment samples were found to be associated with gamma-Proteobacteria, cyanobacteria, and green nonsulfur and low-GC gram-positive bacteria. The first of the above phylotypes cobranches with Shewanella, a well-known iron reducer. This phylogenetic correlation has been exploited to develop culture conditions for thermophilic iron-reducing microorganisms.
Ghosh
Dhritiman
D
Department of Life Science and Biotechnology, Jadavpur University, Calcutta 700 032, India.
Bal
Bijay
B
Kashyap
V K
VK
Pal
Subrata
S
D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D003470:Culture Media; D004275:DNA, Ribosomal; D017753:Ecosystem; D005290:Ferric Compounds / Q000378:metabolism; D005618:Fresh Water / Q000382:microbiology*; D020459:Genes, rRNA; D014644:Genetic Variation; D006358:Hot Temperature; D007194:India; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S; D017422:Sequence Analysis, DNA; D020592:Shewanella / Q000145:classification* / Q000235:genetics
D003470:Culture Media; D004275:DNA, Ribosomal; D005290:Ferric Compounds; D012336:RNA, Ribosomal, 16S