Immunoprotective haem oxygenase induction by ultraviolet A (320-400 nm) radiation in the mouse is inhibited in interferon-gamma null mice.
BACKGROUND
A protective role for the ultraviolet (UV) A waveband against immunosuppression induced by UVB (280-320 nm) radiation has been identified. The mechanism for UVA immunoprotection was found to involve two apparently unrelated mediators, the T-helper-1-associated proinflammatory cytokine interferon (IFN)-gamma and the UVA-induced redox-regulated stress protein, haem oxygenase (HO).


OBJECTIVE
To identify a relationship between these two immune regulators.


METHODS
The HO response to UVA radiation in the skin and liver was examined in mice with a targeted disruption of the IFN-gamma gene, known to be unresponsive to UVA photoimmunoprotection. Results IFN-gamma null mice did not respond to UVA irradiation with the normal upregulation of HO activity in either the irradiated skin or the liver. Injection of these mice with recombinant IFN-gamma previously shown to restore the UVA-photoimmunoprotective effect, here partially and dose-responsively restored their ability for induction of HO activity in both skin and liver following UVA irradiation.


CONCLUSIONS
IFN-gamma appears to be a prerequisite for the immunoprotective induction of HO, although other mediators may also be involved. The UVA responsiveness of HO in an internal organ such as the liver suggests the existence of a soluble UVA-induced mediator from the skin, which may be IFN-gamma.
Reeve
V E
VE
Faculty of Veterinary Science, McMaster Building B14, University of Sydney, NSW 2006, Australia. v.reeve@vetp.usyd.edu.au
Domanski
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D
D000818:Animals; D005260:Female; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism / Q000528:radiation effects*; D007108:Immune Tolerance / Q000528:radiation effects; D007371:Interferon-gamma / Q000494:pharmacology*; D051379:Mice; D018345:Mice, Knockout; D008862:Microsomes, Liver / Q000201:enzymology; D014466:Ultraviolet Rays / Q000009:adverse effects*
D007371:Interferon-gamma; D006419:Heme Oxygenase (Decyclizing)