title: Assessment of utility of meiosis-associated promoters of lily for induction of germinal ds transposition in transgenic rice.
abstract: In order to suppress the somatic excision of the Ds element and increase the independent transposition events of the Ac/Ds transposon tagging system in rice, we employed promoters of two meiosis-specific genes of lily, LIM10 and LIM18. The LIM10 promoter directed GUS expression specifically in anthers, with the LIM18 promoter doing the same in the anthers and somatic tissue. Both promoters induced independent germinal transposition with the frequency of approximately 1%. The LIM10 promoter, lacking induction of somatic transposition, is considered to be useful for improving transposon-tagging efficiencies in rice.
authors.affiliation: Laboratory of Plant Breeding and Genetics, Graduate School of Agricultural Science, Tohoku University, Sendai, 981-8555 Japan.
authors.forename: Ryouhei
authors.initials: R
authors.lastname: Morita
authors.forename: Yoko
authors.initials: Y
authors.lastname: Hattori
authors.forename: Shuji
authors.initials: S
authors.lastname: Yokoi
authors.forename: Hisabumi
authors.initials: H
authors.lastname: Takase
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Minami
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Hiratsuka
authors.forename: Kinya
authors.initials: K
authors.lastname: Toriyama
mesh_terms: D004251:DNA Transposable Elements / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D018525:Germination / Q000235:genetics*; D027762:Lilium / Q000235:genetics*; D008540:Meiosis / Q000235:genetics*; D012275:Oryza / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000254:growth & development / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements