title: Relative affinity constants by electrospray ionization and Fourier transform ion cyclotron resonance mass spectrometry: calmodulin binding to peptide analogs of myosin light chain kinase.
abstract: Synthetic RS20 peptide and a set of its point-mutated peptide analogs have been used to analyze the interactions between calmodulin (CaM) and the CaM-binding sequence of smooth-muscle myosin light chain kinase both in the presence and the absence of Ca(2+). Particular peptides, which were expected to have different binding strengths, were chosen to address the effects of electrostatic and bulky mutations on the binding affinity of the RS20 sequence. Relative affinity constants for protein/ligand interactions have been determined using electrospray ionization and Fourier transform ion cyclotron resonance mass spectrometry. The results evidence the importance of electrostatic forces in interactions between CaM and targets, particularly in the presence of Ca(2+), and the role of hydrophobic forces in contributing additional stability to the complexes both in the presence and the absence of Ca(2+).
authors.affiliation: Department of Chemistry, University of Joensuu, FIN-80101 Joensuu, Finland.
authors.forename: Marjaana
authors.initials: M
authors.lastname: Nousiainen
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Derrick
authors.forename: Daniel
authors.initials: D
authors.lastname: Lafitte
authors.forename: Pirjo
authors.initials: P
authors.lastname: Vainiotalo
mesh_terms: D001665:Binding Sites; D002118:Calcium / Q000737:chemistry*; D002147:Calmodulin / Q000737:chemistry*; D002148:Calmodulin-Binding Proteins / Q000737:chemistry*; D017357:Cyclotrons; D004789:Enzyme Activation; D008274:Magnesium / Q000737:chemistry*; D009219:Myosin-Light-Chain Kinase / Q000737:chemistry*; D011485:Protein Binding; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D002147:Calmodulin; D002148:Calmodulin-Binding Proteins; D009219:Myosin-Light-Chain Kinase; D008274:Magnesium; D002118:Calcium