title: Micromechanical properties of demineralized dentin collagen with and without adhesive infiltration.
abstract: In a previous study, we reported the upper limit of Young's modulus of the unprotected protein at the dentin/adhesive interface to be 2 GPa. In this study, to obtain a more exact value of the moduli of the components at the d/a interface, we used demineralized dentin collagen with and without adhesive infiltration. The prepared samples were analyzed using micro-Raman spectroscopy (micro RS) and scanning acoustic microscopy (SAM). Using an Olympus UH3 SAM (Olympus Co., Tokyo), measurements were recorded with a 400 MHz burst mode lens (120 degrees aperture angle; nominal lateral resolution, 2.5 microm). A series of calibration curves were prepared using the relationship between the ultrasonically measured elastic moduli of a set of known materials and their SAM response. Finally, both the bulk and bar wave elastic moduli were computed for a set of 13 materials, including polymers, ceramics, and metals. These provided the rationale for using extensional wave measurements of the elastic moduli as the basis for extrapolation of the 400 MHz SAM data to obtain Young's moduli for the samples: E = 1.76 +/- 0.00 GPa for the collagen alone; E = 1.84 +/- 0.65 GPa for the collagen infiltrated with adhesive; E = 3.4 +/- 1.00 GPa for the adhesive infiltrate.
authors.affiliation: Department of Biomedical Engineering, Case School of Engineering, Case Western Reserve University, Cleveland, Ohio 44106, USA.
authors.forename: J Lawrence
authors.initials: JL
authors.lastname: Katz
authors.forename: Paulette
authors.initials: P
authors.lastname: Spencer
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Nomura
authors.forename: Ajay
authors.initials: A
authors.lastname: Wagh
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
mesh_terms: D017438:Bisphenol A-Glycidyl Methacrylate / Q000494:pharmacology*; D002138:Calibration; D003094:Collagen / Q000737:chemistry* / Q000302:isolation & purification; D003804:Dentin / Q000737:chemistry*; D017220:Dentin-Bonding Agents / Q000494:pharmacology*; D004548:Elasticity; D004867:Equipment Design; D006801:Humans; D033401:Microscopy, Acoustic / Q000295:instrumentation / Q000592:standards; D008903:Minerals; D008963:Molar; D013059:Spectrum Analysis, Raman
chemical_list: D017220:Dentin-Bonding Agents; D008903:Minerals; C115612:single bond; D017438:Bisphenol A-Glycidyl Methacrylate; D003094:Collagen