[Changes in microcirculation of esophageal mucosa during acute experimental reflux. Pathogenic role of the biliary component].
Our aims were to examine microcirculation during experimental reflux esophagitis in dogs. We compared the effects of microcirculation of the mucosa to 3-hr exposure with acid, mixed acid and bile, we measured the changes in constitutive and inducible nitric oxide synthase activity (cNOS and iNOS).


METHODS
The microcirculation of the upper esophagus was investigated by intravital videomicroscopy. The functional capillary density (FCD), relative vessel area (RVA) and red blood cell velocity (RBCV) were measured. Mucosal barrier integrity was examined by vascular and epithelial permeability indices. Myeloperoxidase (MPO) enzyme activity, cNOS, iNOS activities and microscopic damage were examined in biopsies.


RESULTS
The vascular permeability index, the RBCV and the RVA increased significantly in the treated groups, the FCD significantly decreased after acid exposure. The MPO and iNOS activities were significantly elevated in all treated groups. The cNOS activity did not change after exposure to acid + bile or acid, but significantly decreased after sole bile treatment. Severe mucosal damage was observed after bile exposure.


CONCLUSIONS
Bile induced characteristic microcirculatory changes during experimental reflux esophagitis. Tissue damage and leukocyte infiltration could be exacerbated by bile-induced cNOS inhibition.
Szentpáli
Károly
K
Szegedi Tudományegyetem Altalános Orvostudományi Kar Sebészeti Mútéttani Intézet, Sebészeti Klinika.
Kaszaki
József
J
Erös
Gábor
G
Tiszlavicz
László
L
Paszt
Attila
A
Lázár
György
G
Balogh
Adám
A
Boros
Mihály
M
D000818:Animals; D001646:Bile; D001783:Blood Flow Velocity; D002199:Capillary Permeability; D004195:Disease Models, Animal; D004285:Dogs; D004912:Erythrocytes; D004942:Esophagitis, Peptic / Q000201:enzymology / Q000473:pathology*; D004947:Esophagus / Q000098:blood supply* / Q000201:enzymology; D005764:Gastroesophageal Reflux / Q000201:enzymology / Q000473:pathology*; D008833:Microcirculation; D009092:Mucous Membrane / Q000098:blood supply* / Q000201:enzymology; D019001:Nitric Oxide Synthase / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III
D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III