Separation of proanthocyanidins by degree of polymerization by means of size-exclusion chromatography and related techniques.
The molecular masses of polyphenols in plants and food vary greatly up to the order of 10 kDa. Polymerized polyphenols are not only natural antioxidants but also strong inhibitors of numerous physiological enzymatic activities. Several useful methods for the determination and separation of these high-molecular-mass polyphenols have recently been developed. In this review, details of the methods and applications of size-exclusion chromatographic separation of polymerized polyphenols, particularly those of proanthocyanidins, are described and compared with other related chromatographic or mass spectrometric analyses.
Yanagida
Akio
A
Department of Analytical Chemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1, Horinouchi, Hachioji, Tokyo 192-0392, Japan. yanagida@ps.toyaku.ac.jp
Shoji
Toshihiko
T
Shibusawa
Yoichi
Y
D002845:Chromatography / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019281:Dimerization; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry; D046911:Macromolecular Substances; D013058:Mass Spectrometry / Q000379:methods*; D010636:Phenols / Q000032:analysis / Q000737:chemistry; D011108:Polymers / Q000032:analysis* / Q000737:chemistry*; D059808:Polyphenols; D044945:Proanthocyanidins / Q000032:analysis* / Q000737:chemistry*
D005419:Flavonoids; D046911:Macromolecular Substances; D010636:Phenols; D011108:Polymers; D059808:Polyphenols; D044945:Proanthocyanidins