Hepatoprotective effect of the extract and isocytisoside from Aquilegia vulgaris.
The hepatoprotective effect of the ethanol extract (AvEE) and the main fl avonoid compound 4'-methoxy-5,7-dihydroxy fl avone 6-C-beta-glucopyranoside (isocytisoside, ISOC) from the leaves and stems of Aquilegia vulgaris L. were studied using the CCl(4)-induced hepatotoxicity test. The acute toxicity test in mice showed that AvEE can be classi fi ed as nontoxic since a dose of 3000 mg/ kg did not cause mortality. The barbiturate-induced sleeping time prolonged by CCl(4) administration to mice was signi fi cantly reduced after AvEE treatment proving the protective effect of the extract on microsomal drug-metabolizing enzymes.AvEE and ISOC administered to rats 48 h, 24 h and 2 h before, and 6 h after CCl(4) intoxication caused a signi fi cant decrease in the CCl(4)-induced elevation of hepatic enzymes activity in serum, i.e. sorbitol dehydrogenase (SDH), glutamate oxaloacetate and glutamate pyruvate transaminases (GOT, GPT). Both substances induced CCl(4)-diminished erythrocyte superoxide dismutase (SOD) and reduced the activities of glutathione peroxidase (GPx) and glutathione reductase (GR) preliminarily enhanced by CCl(4). The hepatoprotective properties of AvEE and ISOC were con fi rmed by pathomorphological examination of the liver.
Adamska
T
T
Department of Toxicology, K. Marcinkowski University of Medical Sciences, Dojazd 30, 60-631 Poznań, Poland.
Młynarczyk
W
W
Jodynis-Liebert
J
J
Bylka
W
W
Matławska
I
I
D000818:Animals; D031884:Aquilegia; D002251:Carbon Tetrachloride; D056486:Chemical and Drug Induced Liver Injury / Q000517:prevention & control*; D005260:Female; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008111:Liver Function Tests; D008297:Male; D051379:Mice; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D018515:Plant Leaves; D018547:Plant Stems; D020011:Protective Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D012890:Sleep / Q000187:drug effects
D010936:Plant Extracts; D020011:Protective Agents; D002251:Carbon Tetrachloride