title: Efforts toward creating unnatural base pairs for an expanded genetic code.
abstract: A series of unnatural base pairs was designed and examined for the expansion of the genetic alphabet and for a better understanding of the mechanism of nucleic acid biosyntheses. To improve the shape complementarity of the previously developed unnatural base pairs, 2-amino-6-(N,N-dimethylamino)purine (x)--pyridon-2-one (y) and 2-amino-6-(2-thienyl)purine (s)--y, the pyrimidine analogue, y, was replaced by a five-member ring, 4-imidazolin-2-one (z), and the s-z pairing in replication was examined. Unnatural bases based on the five-member ring were also applied to the development of non-hydrogen-bonded base pairs.
authors.affiliation: Yokoyama CytoLogic Project, ERATO, JST, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Hirao
authors.forename: T
authors.initials: T
authors.lastname: Mitsui
authors.forename: T
authors.initials: T
authors.lastname: Fujiwara
authors.forename: M
authors.initials: M
authors.lastname: Kimoto
authors.forename: T
authors.initials: T
authors.lastname: To
authors.forename: T
authors.initials: T
authors.lastname: Okuni
authors.forename: A
authors.initials: A
authors.lastname: Sato
authors.forename: Y
authors.initials: Y
authors.lastname: Harada
authors.forename: S
authors.initials: S
authors.lastname: Yokoyama
mesh_terms: D020029:Base Pairing; D004261:DNA Replication; D005815:Genetic Code; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D011685:Purine Nucleotides / Q000737:chemistry; D011742:Pyrimidine Nucleotides / Q000737:chemistry
chemical_list: D009841:Oligonucleotides; D011685:Purine Nucleotides; D011742:Pyrimidine Nucleotides