Genetic analysis and genome mapping in Raphanus.
The first genetic map of the Raphanus genome was developed based on meiosis in a hybrid between Raphanus sativus (cultivated radish) and Raphanus raphanistrum (wild radish). This hybrid was used to produce a BC1 population of 54 individuals and an F2 population of 85 individuals. A total of 236 marker loci were assayed in these populations using a set of 144 informative Brassica RFLP probes previously used for genetic mapping in other crucifer species. The genetic maps derived from the BC1 and F2 populations were perfectly collinear and were integrated to produce a robust Raphanus map. Cytological observations demonstrated strict bivalent pairing in the R. sativus x R. raphanistrum hybrids. Productive pairing along the length of each chromosome was confirmed by the identification of nine extensive linkage groups and the lack of clustering of marker loci. Indeed, the distributions of both marker loci and crossovers was more random than those reported for other crop species. The genetic markers and the reference map of Raphanus will be of considerable value for future trait mapping and marker-assisted breeding in this crop, as well as in the intergenomic transfer of Raphanus genes into Brassica crops. The future benefits of comparative mapping with Arabidopsis and Brassica species are also discussed.
Bett
Kirstin E
KE
Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Pl., Saskatoon, SK S7N OX2. k.bett@usask.ca
Lydiate
Derek J
DJ
D002874:Chromosome Mapping; D003433:Crosses, Genetic; D005787:Gene Frequency; D012150:Polymorphism, Restriction Fragment Length; D031224:Raphanus / Q000235:genetics*