[Divinyl sulfone as a cross-linking reagent for oligomeric proteins].
The kinetics of the nucleophilic addition reactions of divinyl sulfone to amino groups of glycine and model proteins was studied in aqueous solution at 30 degrees C. The rate constants for glycine, bovine serum albumin, and alpha 1-casein were (4.84 +/- 0.58) x 10(-1), (2.97 +/- 0.31) x 10(-2), and (2.38 +/- 0.49) x 10(-2) M-1s-1, respectively. Divinyl sulfone was proposed as a cross-linking reagent for the qualitative detection of protein association in solution. The cross-linking capacity of divinyl sulfone was compared to that of 1,3,5-triacryloylhexahydro-s-triazine.
Sereĭkaĭte
I
I
Faculty of Fundamental Sciences, Gediminas Technical University, Vilnius, Sauletekio al. 11, Vilnius, 2040 Lithuania. sjolanta@fm.vtu.lt
Bassus
D
D
Bobnis
R
R
Denis
G
G
Bumialene
Zh
Zh
Bumialis
B A
BA
D001671:Biochemistry / Q000379:methods*; D002364:Caseins / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry*; D005998:Glycine / Q000737:chemistry; D007700:Kinetics; D011506:Proteins / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012996:Solutions; D013450:Sulfones / Q000737:chemistry*; D014227:Triazines / Q000737:chemistry
D002364:Caseins; D003432:Cross-Linking Reagents; D011506:Proteins; D012996:Solutions; D013450:Sulfones; D014227:Triazines; D012710:Serum Albumin, Bovine; C009873:divinyl sulfone; C049485:1,3,5-triacryloylhexahydrotriazine; D005998:Glycine