title: The prognosis in spindle-cell sarcoma depends on the expression of cyclin-dependent kinase inhibitor p27(Kip1) and cyclin E.
abstract: The aim of the present study was to examine the prognostic significance of p27(Kip1) and cyclin E expression in patients with spindle-cell soft tissue sarcomas. In 46 cases of spindle-cell sarcoma including 17 pre-operative biopsy materials, the expression of p27(Kip1) and cyclin E was immunohistochemically examined. The expression of p27(Kip1) decreased in the nuclei of metastatic primary tumor cells (stage IV), whereas the expression of cyclin E increased in those lesions. On univariate analysis, when the expression of p27(Kip1) and cyclin E was analyzed together, patients with spindle-cell sarcoma exhibiting low expression of p27(Kip1) and high expression of cyclin E showed lower distant-metastasis-free survival (DMFS) and overall survival (OS) than those with other combinations of the two parameters (both P < 0.0001). Multivariate analysis revealed that patients with low p27(Kip1) and high cyclin E expression also showed a decrease in DMFS (P = 0.0007, relative risk = 21.3) and OS (P = 0.005, relative risk = 20.8). These results suggest that the combination analysis of p27(Kip1) and cyclin E expression even in biopsy specimens allows the prediction of the clinical behavior of spindle-cell sarcoma.
authors.affiliation: Department of Pathology and Orthopedic Surgery, Yamaguchi University School of Medicine, Ube, Yamaguchi 755-8505, Japan. goto123@hyper.ocn.ne.jp
authors.forename: Yoshinari
authors.initials: Y
authors.lastname: Goto
authors.forename: Shigeto
authors.initials: S
authors.lastname: Kawauchi
authors.forename: Koichiro
authors.initials: K
authors.lastname: Ihara
authors.forename: Kenzo
authors.initials: K
authors.lastname: Ikemoto
authors.forename: Ritsuko
authors.initials: R
authors.lastname: Ohi
authors.forename: Shinya
authors.initials: S
authors.lastname: Kawai
authors.forename: Kohsuke
authors.initials: K
authors.lastname: Sasaki
mesh_terms: D018797:Cell Cycle Proteins / Q000378:metabolism*; D019927:Cyclin E / Q000378:metabolism*; D050760:Cyclin-Dependent Kinase Inhibitor p27; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D012509:Sarcoma / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D012983:Soft Tissue Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D015996:Survival Rate; D025521:Tumor Suppressor Proteins / Q000378:metabolism*
chemical_list: D018797:Cell Cycle Proteins; D019927:Cyclin E; D025521:Tumor Suppressor Proteins; D050760:Cyclin-Dependent Kinase Inhibitor p27