Preliminary study on the cooperation of IL-6 and mB7-1 in the induction of effective antitumor immunity in vitro.
To find a new way for gene therapy against tumors with weak immunogenicity, the effect of mB7-1 costimulation alone, or combined with IL-6, in inducing antitumor immunity in vitro was investigated. It was found that mB7-1 cD-NA transfected B16 cells (B16-mB7-1) induced the expansion of effector lymphocytes and the generation of specific lytic activity more effectively than wild type B16 melanoma cells (B16-wt) or mock-transfected B16 cells (B16-neo) did. (P < 0.01), IL-6 could effectively stimulate lymphocytes proliferation, but failed to enhance its cytotoxicity, while the combination of mB7-1 and IL-6 increased both lymphocyte proliferative response and T-cell-mediated cytotoxicity more significantly than B7-1 or IL-6 did alone (P < 0.01). It was inferred that the costimulatory molecule B7-1 is required for the activation and proliferation of T lymphocytes; the expression of mB7-1 in tumor cells could increase their immunogenicity and induce effective antitumor immune response, and the combination of B7-1 and IL-6 could induce more effective antitumor immunity, indicating that cooperation of IL-6 and mB7-1 plays a role in T lymphocyte activation.
Qu
S
S
Department of Biochemistry, School of Basic Medical Sciences, Tongji Medical University, Wuhan 430030.
Liu
R
R
Wang
Y
Y
Wang
J
J
D000818:Animals; D000970:Antineoplastic Agents / Q000276:immunology / Q000494:pharmacology; D018122:B7-1 Antigen / Q000276:immunology* / Q000494:pharmacology; D017077:Culture Media, Conditioned; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D007167:Immunotherapy; D015850:Interleukin-6 / Q000276:immunology* / Q000494:pharmacology; D008546:Melanoma, Experimental / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D018122:B7-1 Antigen; D017077:Culture Media, Conditioned; D015850:Interleukin-6