title: [FCM detection and pathology observation of 5 mm cutting-edge of laryngocarcinoma].
abstract: OBJECTIVE
  To explore the safety of 5 mm cutting-edges with the analysis of DNA contend and cell cycle and the pathology observation.
  
  
  METHODS
  Twenty specimens of laryngocarcinoma and its cutting-edges were analyzed by flow cytometry, contrasting to 5 samples of normal larynx tissue, and their light-microscopy and electra-microscopy were discussed.
  
  
  RESULTS
  The DNA aneuploidy(AN) rate in the laryngocarcinoma, its cutting-edges and the normal tissue is respectively 60% (12/20), 15%(3/20) and 0. The rate of S phrase of the laryngocarcinoma and its cutting-edges is respectively 20.33 +/- 6.0 and 12.35 +/- 3.81, so the DI 1.78 +/- 0.82 and 1.07 +/- 0.04, PI 29.09 +/- 6.8 and 11.75 +/- 3.75, there are significant differences (P < 0.05). The light-microscopy showed 5 mm cutting-edges were infiltrated by carcinoma in the form of submucosa or extention of carcinoma in situ and the results was, to some extension, consistent with the findings of FCM.
  
  
  CONCLUSIONS
  The safety of 5 mm cutting-edge varies depending on whether invading paraglottic space.
authors.affiliation: Department of Otorhinolaryngology, Anhui Province Hospital, Hefei 230001.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Chen
authors.forename: Jingwu
authors.initials: J
authors.lastname: Sun
authors.forename: Yanming
authors.initials: Y
authors.lastname: Hu
authors.forename: Feichang
authors.initials: F
authors.lastname: Ye
authors.forename: Mingshan
authors.initials: M
authors.lastname: Wang
authors.forename: Zicang
authors.initials: Z
authors.lastname: Jin
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Huang
authors.forename: Demin
authors.initials: D
authors.lastname: Han
mesh_terms: D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology* / Q000601:surgery; D002453:Cell Cycle; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics / Q000473:pathology* / Q000601:surgery; D008297:Male; D008875:Middle Aged
chemical_list: D004273:DNA, Neoplasm