title: Crystal structure of an RNA purine-rich tetraplex containing adenine tetrads: implications for specific binding in RNA tetraplexes.
abstract: Purine-rich regions in DNA and RNA may contain both guanines and adenines, which have various biological functions. Here we report the crystal structure of an RNA purine-rich fragment containing both guanine and adenine at 1.4 A resolution. Adenines form an adenine tetrad in the N6-H em leader N7 conformation. Substitution of an adenine tetrad in the guanine tetraplex does not change the global conformation but introduces irregularity in both the hydrogen bonding interaction pattern in the groove and the metal ion binding pattern in the central cavity of the tetraplex. The irregularity in groove binding may be critical for specific binding in tetraplexes. The formation of G-U octads provides a mechanism for interaction in the groove. Ba(2+) ions prefer to bind guanine tetrads, and adenine tetrads can only be bound by Na(+) ions, illustrating the binding selectivity of metal ions for the tetraplex.
authors.affiliation: Departments of Chemistry and Biochemistry, The Ohio State University, 200 Johnston Lab, 176 West 19th Avenue, Columbus, OH 43210, USA.
authors.forename: Baocheng
authors.initials: B
authors.lastname: Pan
authors.forename: Yong
authors.initials: Y
authors.lastname: Xiong
authors.forename: Ke
authors.initials: K
authors.lastname: Shi
authors.forename: Junpeng
authors.initials: J
authors.lastname: Deng
authors.forename: Muttaiya
authors.initials: M
authors.lastname: Sundaralingam
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D008670:Metals / Q000378:metabolism; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry* / Q000378:metabolism
chemical_list: D008670:Metals; D012313:RNA