Adenosine-rich elements present in the 5'-untranslated region of PABP mRNA can selectively reduce the abundance and translation of CAT mRNAs in vivo.
The poly(A)-binding protein (PABP) is a highly conserved eukaryotic protein whose synthesis is regulated at the post-transcriptional level. The binding of PABP to the poly(A)-rich element found in the 5'-untranslated region (5'UTR) of PABP mRNA specifically inhibits its own translation. In this report, we show that similar adenosine-rich elements in the 5'UTR of the chloramphenicol acetyl-transferase (CAT) gene can significantly reduce the reporter mRNA abundance and translation in human 293 cells. The reduction in mRNA level, but not CAT expression, is dependent on the size of the 5'UTR poly(A) element. Furthermore, one 5'UTR-tethered PABP molecule is enough to inhibit CAT expression without affecting its mRNA level. We propose that the control of PABP synthesis may involve mRNA decay and the repression of translation.
Melo
Eduardo O
EO
Departamento de Biologia Celular, Universidade de Brasília, Asa Norte, Brasília, DF 70910-900, Brazil. eom@cenargen.embrapa.br
de Melo Neto
Osvaldo P
OP
Martins de Sá
Cezar
C
D020121:5' Untranslated Regions; D000241:Adenosine / Q000378:metabolism*; D001483:Base Sequence; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics*; D017931:DNA Primers; D017930:Genes, Reporter; D006801:Humans; D008969:Molecular Sequence Data; D039102:Poly(A)-Binding Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics*
D020121:5' Untranslated Regions; D017931:DNA Primers; D039102:Poly(A)-Binding Proteins; D012333:RNA, Messenger; D015500:Chloramphenicol O-Acetyltransferase; D000241:Adenosine