title: Preliminary crystallographic characterization of the human beta2 microglobulin His31Tyr mutant in a tetrameric assembly.
abstract: Patients receiving prolonged haemodialysis treatment are exposed to a variety of arthropathies and bone lesions arising from deposition of amyloid material in the skeletal system. beta2 microglobulin is the 11.7 kDa light chain of the class I major histocompatibility complex, from which it is normally released to plasmatic fluids, transported to kidneys and excreted. Owing to renal failure it accumulates, giving rise to dialysis-related amyloidosis, a severe disease found in patients receiving dialysis for several years. The three-dimensional structure of beta2 microglobulin is known to be based on a seven-stranded beta-sandwich fold, typical of the class C immunoglobulin superfamily. Analysis of the protein fold in different mutants and/or crystal environments and of its structural stability may help in understanding the molecular bases of amyloid fibril formation and of diseases related to protein misfolding. Here, the preliminary crystallographic analysis of the His31Tyr beta2 microglobulin mutant, designed to abolish the copper-ion binding observed in the wild-type protein, is presented. The protein mutant displays increased fold stability, faster folding kinetics and crystallizes in the tetragonal C222(1) space group, with unit-cell parameters a = 105.2, b = 150.2, c = 93.7 A and four molecules per asymmetric unit.
authors.affiliation: Department of Physics-INFM and Center of Excellence for Biomedical Research, University of Genova, Via Dodecaneso 33, 16146 Genova, Italy.
authors.forename: Simone
authors.initials: S
authors.lastname: Zuccotti
authors.forename: Camillo
authors.initials: C
authors.lastname: Rosano
authors.forename: Palma
authors.initials: P
authors.lastname: Mangione
authors.forename: Vittorio
authors.initials: V
authors.lastname: Bellotti
authors.forename: Martino
authors.initials: M
authors.lastname: Bolognesi
mesh_terms: D003300:Copper; D003460:Crystallization; D019281:Dimerization; D006801:Humans; D016297:Mutagenesis, Site-Directed; D020125:Mutation, Missense; D017510:Protein Folding; D014961:X-Ray Diffraction; D001613:beta 2-Microglobulin / Q000737:chemistry* / Q000235:genetics
chemical_list: D001613:beta 2-Microglobulin; D003300:Copper