Spectroscopic studies on poly(ethylene glycol)-lysozyme interactions.
In the present paper, different spectroscopic methods were applied to evaluate conformational changes of hen egg-white lysozyme (HEWL) in various solvents and in the presence of poly(ethylene glycol) (PEG). In citrate (0.007M, pH=6), or in Tris (0.1M, pH=7.4), no conformational change of the protein was measured across the range of concentrations tested. In addition, HEWL in ultra-pure water revealed no irreversible conformational change and no activity loss, at least at low concentrations (< or =0.2mg/ml). Whereas PEG can induce a reorganization of water molecules, no change of the secondary and tertiary protein conformations was observed in the presence of PEG. In addition, in the presence of PEG of various molecular weights, no change of enzymatic activity of the HEWL was observed across the range of concentrations tested.
Malzert
A
A
Inserm ERIT-M 0104 Ingénierie de la Vectorisation Particulaire, Immeuble IBT, 10, rue André Boquel, 49100 Angers, France.
Boury
F
F
Renard
D
D
Robert
P
P
Lavenant
L
L
Benoît
J P
JP
Proust
J E
JE
D002942:Circular Dichroism; D019343:Citric Acid; D004337:Drug Carriers; D008027:Light; D008835:Micrococcaceae / Q000187:drug effects; D008970:Molecular Weight; D009113:Muramidase / Q000737:chemistry* / Q000494:pharmacology; D009391:Nephelometry and Turbidimetry; D011092:Polyethylene Glycols / Q000737:chemistry*; D025341:Principal Component Analysis; D011487:Protein Conformation; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared; D014325:Tromethamine; D014867:Water
D004337:Drug Carriers; D014325:Tromethamine; D014867:Water; D019343:Citric Acid; D011092:Polyethylene Glycols; C421864:hen egg lysozyme; D009113:Muramidase