title: First isolation and identification of Rickettsia conorii from ticks collected in the region of Fokida in Central Greece.
abstract: Three different spotted-fever group rickettsiae-Rickettsia conorii, R. massiliae, and R. rhipicephali-were detected and identified by PCR-restriction fragment length polymorphism analysis in Rhipicephalus ticks collected from domestic animals in the Fokida region of Greece, where a high seroprevalence of antibodies to R. conorii was previously demonstrated. The infection rate of ticks was 1.6%. Moreover, R. conorii was isolated from one Rhipicephalus sanguineus tick.
authors.affiliation: Laboratory of Clinical Bacteriology, Parasitology, Zoonoses, and Geographical Medicine, Collaborating Center of the World Health Organization, University of Heraklion, 71409 Crete, Greece. annapsa@med.uoc.gr
authors.forename: Anna
authors.initials: A
authors.lastname: Psaroulaki
authors.forename: Ioanna
authors.initials: I
authors.lastname: Spyridaki
authors.forename: Alexandros
authors.initials: A
authors.lastname: Ioannidis
authors.forename: Thomas
authors.initials: T
authors.lastname: Babalis
authors.forename: Achilleas
authors.initials: A
authors.lastname: Gikas
authors.forename: Yannis
authors.initials: Y
authors.lastname: Tselentis
mesh_terms: D000818:Animals; D000829:Animals, Domestic / Q000469:parasitology; D015373:Bacterial Typing Techniques; D002522:Chlorocebus aethiops; D004269:DNA, Bacterial / Q000032:analysis; D006115:Greece; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D020114:Rickettsia conorii / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D013984:Tick Infestations / Q000469:parasitology; D013987:Ticks / Q000145:classification / Q000382:microbiology*; D014709:Vero Cells
chemical_list: D004269:DNA, Bacterial