title: Antibodies in proteomics II: screening, high-throughput characterization and downstream applications.
abstract: There are many ways in which the use of antibodies and antibody selection can be improved and developed for high-throughput characterization. Standard protocols, such as immunoprecipitation, western blotting and immunofluorescence, can be used with antibody fragments generated by display technologies. Together with novel approaches, such as antibody chips and intracellular immunization, these methods will yield useful proteomic data following adaptation of the protocols for increased reliability and robustness. To date, most work has focused on the use of standard, well-characterized commercial antibodies. Such protocols need to be adapted for broader use, for example, with antibody fragments or other binders generated by display technologies, because it is unlikely that traditional approaches will provide the required throughput.
authors.affiliation: B Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA. amb@lanl.gov
authors.forename: Andrew
authors.initials: A
authors.lastname: Bradbury
authors.forename: Nileena
authors.initials: N
authors.lastname: Velappan
authors.forename: Vittorio
authors.initials: V
authors.lastname: Verzillo
authors.forename: Milan
authors.initials: M
authors.lastname: Ovecka
authors.forename: Leslie
authors.initials: L
authors.lastname: Chasteen
authors.forename: Daniele
authors.initials: D
authors.lastname: Sblattero
authors.forename: Roberto
authors.initials: R
authors.lastname: Marzari
authors.forename: Jianlong
authors.initials: J
authors.lastname: Lou
authors.forename: Robert
authors.initials: R
authors.lastname: Siegel
authors.forename: Peter
authors.initials: P
authors.lastname: Pavlik
mesh_terms: D000906:Antibodies / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D007118:Immunoassay / Q000379:methods*; D019151:Peptide Library; D040081:Protein Array Analysis / Q000379:methods*; D015202:Protein Engineering / Q000379:methods*; D040901:Proteomics / Q000379:methods*
chemical_list: D000906:Antibodies; D019151:Peptide Library