[Restoring beta1 integrin activation function in K562 cells transfected with antisense VEGF121 cDNA].
To investigate the effect of vascular endothelial growth factor (VEGF) on beta1 integrin (VLA-4 and VLA-5) activation ability in K562 cells transfected with antisense VEGF121 cDNA, K562 cells were transfected with antisense (As), sense (S) and vector (V, pcDNA(3)). Flow cytometry was used to evaluate the expression rate of VLA-4 (CD49d/CD29) and VLA-5 (CD49e/CD29) and beta1 integrin activation ability in the transfected K562 cells. The results showed that the expression rates of VLA-4 and VLA-5 were more than 92% in the transfected K562 cells and there was no difference among the K562/V, K562/S and K562/As cells. However, beta1 integrin activated 9EG7 expression rate in K562/As cells was higher than that in K562/V cells [(75.6 +/- 10.5)% vs (41.2 +/- 2.1)%, P < 0.01)] after activation with beta1 integrin activator 8A2. It is concluded that function of beta1 integrin to be activated is restored in K562 cells transfected with antisense VEGF121 cDNA.
Ruan
Guo-Rui
GR
People's Hospital and Institute of Hematology, Peking University, Beijing 100044, China. celiar@public3.bta.net.cn
Liu
Yan-Rong
YR
Chen
Shan-Shan
SS
Yu
Hong
H
Chang
Yan
Y
Bai
Ren-Kui
RK
Fu
Jia-Yu
JY
D004247:DNA / Q000235:genetics; D016373:DNA, Antisense / Q000235:genetics*; D016228:Endothelial Growth Factors / Q000235:genetics* / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D039041:Integrin alpha4beta1 / Q000096:biosynthesis*; D039081:Integrin alpha5beta1 / Q000096:biosynthesis*; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism; D020014:K562 Cells; D008222:Lymphokines / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D016373:DNA, Antisense; D016228:Endothelial Growth Factors; D039041:Integrin alpha4beta1; D039081:Integrin alpha5beta1; D036341:Intercellular Signaling Peptides and Proteins; D008222:Lymphokines; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D004247:DNA