[Establishment and identification of highly expressing and replicating hepatitis B virus genome transgenic mouse models].
OBJECTIVE
To establish a highly expressing and replicating hepatitis B virus (HBV) genome transgenic mouse models for screening anti-HBV drugs and investigating the pathogenesis of hepatitis B.


METHODS
Elongated HBV genome as the investigated gene was transducted into the pronuclei of the fertilized eggs of mice by the technique of microinjection, then the eggs were transplanted into the oviducts of the pseudopregnant mice. All the newborn mice were screened and identified by PCR and Southern blot detecting genomic DNA in tail tissue, then the positive mice were examined plasma HBsAg, HBeAg by ELISA and plasma HBV DNA by Southern blot.


RESULTS
Among the 61 offsprings, 18 were positive for tail tissue HBV DNA examination, 7 of which were positive for replication and expression detection.


CONCLUSIONS
Transgenic mice with elongated HBV genome possess high efficiency of replication and expression, which can be used for further investigation.
Wu
Jin ming
Jm
Institute of Liver Diseases, Tongji Hospital, Tongji Medical College, Huazhong Science and Technology University, Wuhan 430030, China.
Lin
Ju-sheng
JS
Chen
Bi-tao
BT
Zheng
Xin-min
XM
Zhao
Hao-bin
HB
Liang
Kuo-huan
KH
D000818:Animals; D004261:DNA Replication; D004279:DNA, Viral / Q000235:genetics; D004195:Disease Models, Animal; D016679:Genome, Viral; D006509:Hepatitis B / Q000821:virology; D006514:Hepatitis B Surface Antigens / Q000235:genetics; D006513:Hepatitis B e Antigens / Q000235:genetics; D006515:Hepatitis B virus / Q000235:genetics* / Q000502:physiology; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics*; D014779:Virus Replication
D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens