Spectrophotometric determination of a thiobarbituric acid-reactive substance in human hair.
A simple and sensitive spectrophotometric method for the determination of a thiobarbituric acid-reactive substance (TBARS) in human hair has been developed. The proposed method is based on the formation of a red-colored product by the reaction of products of lipid peroxidation with thiobarbituric acid in an acidic medium. The absorbance of the resulting red product was measured at 534 nm. The linear dynamic range was between 1.0 and 20 micromol/L. The recoveries were 98.3-105.0%, and the relative standard deviations (RSD) were 0.32-1.24, respectively. TBARS in digested hair sample was stable for 3 days at room temperature. It was found that, using this method, the hair TBARS concentration in smokers (0.116 +/- 0.030 micromol/g, n = 30) was significantly higher than that in non-smokers (0.096 +/- 0.015 micromol/g, n = 30) (p < 0.05).
Sheu
Jenn-Yuan
JY
Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
Chen
Po-Hua
PH
Tseng
Wei-Chang
WC
Chen
Chang-Yu
CY
Tsai
Li-Yu
LY
Huang
Yeou-Lih
YL
D002084:Butylated Hydroxytoluene; D004355:Drug Stability; D006197:Hair / Q000737:chemistry*; D006801:Humans; D007202:Indicators and Reagents; D015227:Lipid Peroxidation; D010756:Phosphoric Acids / Q000737:chemistry; D012907:Smoking / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis*
D007202:Indicators and Reagents; D010756:Phosphoric Acids; D017392:Thiobarbituric Acid Reactive Substances; D002084:Butylated Hydroxytoluene; C030242:phosphoric acid