Effect of the flavonoid components obtained from Scutellaria radix on the histamine, immunoglobulin E and lipid peroxidation of spleen lymphocytes of Sprague-Dawley rats.
The effect on the IgE content induced by concanavalin A in spleen lymphocytes of the presence wogonin, ganhuangenin, wogonoside and 3,5,7,2',6'-pentahydroxyl flavanone was investigated. These flavonoid components markedly inhibited the histamine released from cells stimulated with the calcium ionophore, A23187. However, the magnitude of the inhibitory effect on the degree of lipid peroxidation by ConA of these components was in order of PHF>GHG>WG>WGS. Interestingly, WG, GHG and WGS, with a methoxyl group in the A and B rings, strongly inhibited histamine and IgE production, whereas PHF without a methoxyl group was much stronger than that for lipid peroxidation. We suggest that WG, GHG and WGS might block the pathway for the release of histamine, and that the IgE level in spleen lymphocytes is responsible for the lipid peroxidation.
Lim
Beong Ou
BO
Graduate School of East-West Medical Science, Department of Medical Nutrition, Kyung Hee University, 1 Hoeki-Dong, Dongdaemoon-Ku, Seoul 130-701, Korea. beongou@khu.ac.kr
Choue
Ryo Won
RW
Lee
Hyeon Yong
HY
Seong
Nak Sul
NS
Kim
Jong Dai
JD
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology; D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay; D005419:Flavonoids / Q000494:pharmacology*; D006632:Histamine / Q000378:metabolism*; D007073:Immunoglobulin E / Q000378:metabolism*; D037102:Lectins / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D031363:Scutellaria / Q000737:chemistry*; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
D003470:Culture Media; D005419:Flavonoids; D037102:Lectins; D003208:Concanavalin A; D007073:Immunoglobulin E; D000001:Calcimycin; D006632:Histamine