Neurobehavioral assessment of transplanted porcine Sertoli cells into the intact rat striatum.
Sertoli cells, a testes-derived cell with immunosuppressive and trophic properties, may serve as an alternative cell source for transplantation in a number of neurodegenerative diseases. However, before Sertoli cells can be considered for clinical use, safety studies must be conducted to ensure that the cells themselves produce no adverse effects when transplanted into the central nervous system. The present study assessed the behavioral effects of transplanting porcine Sertoli cells into the striatum of normal rats and provided a histological examination of the graft site and host striatum. Activity monitors revealed significant increases in nocturnal locomotor activity over time following both sham and Sertoli transplants. Ambulation and rearing, but not stereotypic measures, were increased compared to pre-transplant levels. Sertoli animals exhibited less behavioral alteration than sham controls. Histological examination of the striatum demonstrated surviving Sertoli cell transplants in an intact striatum. These results indicated that Sertoli cell xenografts might be a safe alternative cell source for neurotransplantation procedures requiring immune or trophic support.
Rodríguez
Alba I
AI
Department of Neurosurgery and The Neurosciences Program, Center for Aging and Brain Repair, College of Medicine, University of South Florida, Tampa, FL 33612, USA. arodrig1@hfhs.org
Willing
Alison E
AE
Cameron
Don F
DF
Saporta
Samuel
S
Sandberg
Paul R
PR