title: [Construction of recombinant pEGFP/hTRT plasmid].
abstract: OBJECTIVE
  To construct a plasmid which has a reporter gene for exploring the role of human telomerase reverse transcriptase(hTRT) in in-vitro cell cultivation.
  
  
  METHODS
  hTRT was cut by restricted enzyme from plasmid pGRN145 and inserted to plasmid pEGFP-C1 (enhanced green fluorescent protein).
  
  
  RESULTS
  Restricted enzyme analysis and DNA sequencing showed that the sequence of the pEGFP -hTRT transgenic plasmid was correct.
  
  
  CONCLUSIONS
  The recombinant vector pEGFP-hTRT has been successfully constructed, and it can be used as a transgenic plasmid in generating immortalized cell lines.
authors.affiliation: Department of Oral Medicine, Ninth People's Hospital, Shanghai Second Medical University, Shanghai, P. R. China 200011.
authors.forename: Hai-wen
authors.initials: HW
authors.lastname: Zhou
authors.forename: Zeng-tong
authors.initials: ZT
authors.lastname: Zhou
authors.forename: Wen-jun
authors.initials: WJ
authors.lastname: Yang
authors.forename: De-li
authors.initials: DL
authors.lastname: Liu
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.forename: Hong-liang
authors.initials: HL
authors.lastname: Hu
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Lei
authors.initials: L
authors.lastname: Cui
authors.forename: Yi-lin
authors.initials: YL
authors.lastname: Cao
mesh_terms: D004268:DNA-Binding Proteins; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics; D019098:Telomerase / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D019098:Telomerase