title: New CMS-associated phenotypes in cybrids Nicotiana tabacum L. (+Hyoscyamus niger L.).
abstract: Morphological characteristics were studied in cytoplasmic male sterile (CMS) cybrids possessing the tobacco nuclear genome, Hyoscyamus niger plastome and recombinant mitochondria. After backcrosses with tobacco, new flower modifications were found, including: conversions of stamens into branched filamentous structures; alterations in the shape of petals and the corolla limb; and high degrees of reduction in most flower organs. Vegetative alterations (leaf elongation and stem branching) occurred in some cybrids. Results confirmed that a protoplast fusion-based alloplasmic cytoplasm transfer, followed by conventional backcrosses, is a useful tool for generating alternative CMS sources with novel nucleo-cytoplasmic compositions. These alterations in the genetic status were accompanied by modified floral and vegetative phenotypes.
authors.affiliation: International Institute of Cell Biology, Zabolotnogo Str. 148, 252143 Kiev-143, Ukraine.
authors.forename: Mikhajlo K
authors.initials: MK
authors.lastname: Zubko
authors.forename: Elena I
authors.initials: EI
authors.lastname: Zubko
authors.forename: Klaus
authors.initials: K
authors.lastname: Adler
authors.forename: Bernhard
authors.initials: B
authors.lastname: Grimm
authors.forename: Yuri Yu
authors.initials: YY
authors.lastname: Gleba
mesh_terms: D003593:Cytoplasm / Q000235:genetics*; D035264:Flowers / Q000033:anatomy & histology* / Q000235:genetics* / Q000502:physiology; D006824:Hybridization, Genetic; D010641:Phenotype; D018515:Plant Leaves / Q000033:anatomy & histology / Q000235:genetics; D018547:Plant Stems / Q000033:anatomy & histology / Q000235:genetics; D012098:Reproduction; D014026:Nicotiana / Q000033:anatomy & histology* / Q000166:cytology / Q000235:genetics* / Q000502:physiology