[Construction of the eukaryotic expression vector PsecTaq2A-AMG for human amelogenin].
OBJECTIVE
The purpose of this study was to construct a eukaryotic expression vector for human amelogenin (AMG).


METHODS
PCR was performed to amplify the AMG encoding region. Amplified fragments for human AMG were recovered and inserted into eukaryotic expression vectors PsecTaq2A. The recombinant plasmid PsecTaq2A-AMG was constructed and their positive clones were identified.


RESULTS
1. Amplified products were checked by electrophoresis and the results were satisfactory. 2. The recombinant plasmid PsecTaq2A-AMG was analyzed by restriction endonuclease mapping and DNA sequencing. The results of sequencing were consistent with those from GenBank.


CONCLUSIONS
The recombinant plasmid PsecTaq2A-AMG was successfully constructed with properly inserted DNA sequence encoding mature amelogenin.
Yang
Ailing
A
Oral Medicine of West China College of Stomatology, Sichuan University, Chengdu 610041, China.
Xu
Chenrong
C
Zhang
Jincai
J
D053523:Amelogenin; D002999:Clone Cells / Q000378:metabolism; D003001:Cloning, Molecular; D004274:DNA, Recombinant / Q000096:biosynthesis / Q000235:genetics; D003746:Dental Enamel Proteins / Q000096:biosynthesis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005057:Eukaryotic Cells / Q000378:metabolism*; D005822:Genetic Vectors; D006801:Humans; D010957:Plasmids / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
C507199:AMELX protein, human; D053523:Amelogenin; D004274:DNA, Recombinant; D003746:Dental Enamel Proteins; D011994:Recombinant Proteins