title: Structural basis for thermostability of beta-glycosidase from the thermophilic eubacterium Thermus nonproteolyticus HG102.
abstract: The three-dimensional structure of a thermostable beta-glycosidase (Gly(Tn)) from the thermophilic eubacterium Thermus nonproteolyticus HG102 was determined at a resolution of 2.4 A. The core of the structure adopts the (betaalpha)(8) barrel fold. The sequence alignments and the positions of the two Glu residues in the active center indicate that Gly(Tn) belongs to the glycosyl hydrolases of retaining family 1. We have analyzed the structural features of Gly(Tn) related to the thermostability and compared its structure with those of other mesophilic glycosidases from plants, eubacteria, and hyperthermophilic enzymes from archaea. Several possible features contributing to the thermostability of Gly(Tn) were elucidated.
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.
authors.forename: Xinquan
authors.initials: X
authors.lastname: Wang
authors.forename: Xiangyuan
authors.initials: X
authors.lastname: He
authors.forename: Shoujun
authors.initials: S
authors.lastname: Yang
authors.forename: Xiaomin
authors.initials: X
authors.lastname: An
authors.forename: Wenrui
authors.initials: W
authors.lastname: Chang
authors.forename: Dongcai
authors.initials: D
authors.lastname: Liang
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis / Q000378:metabolism; D001665:Binding Sites; D003460:Crystallization; D018360:Crystallography, X-Ray; D004795:Enzyme Stability; D006026:Glycoside Hydrolases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D006358:Hot Temperature; D006860:Hydrogen Bonding; D057927:Hydrophobic and Hydrophilic Interactions; D007477:Ions; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017510:Protein Folding; D017433:Protein Structure, Secondary; D016415:Sequence Alignment; D013329:Structure-Activity Relationship; D013824:Thermus / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D001617:beta-Glucosidase
chemical_list: D000596:Amino Acids; D007477:Ions; D006026:Glycoside Hydrolases; D001617:beta-Glucosidase; C441633:beta-glycosidase, Thermus nonproteolyticus