[Antisense oligodeoxynucleotides of human telomerase reverse transcriptase inhibit endometrial carcinoma cell HEC-1A proliferation].
OBJECTIVE
To evaluate antisense technology for human telomerase inhibition in the treatment of endometrial cancer.


METHODS
An antisense oligodeoxynucleotides (AODN) directed against the human telomerase transcriptase (hTERT), designed and synthesized to serve as a telomerase inhibitor, was transfected into endometrial carcinoma cell line HEC-1A by lipofectin. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blot were used to test the expression of hTERT mRNA and hTERT protein before and after transfection. Telomerase activity was tested by telomeric repeat amplification protocol. The proliferation and growth of HEC-1A were also studied by methyl thiazolyl tetrazolium and cell growth curve before and after transfection.


RESULTS
AODN could down-regulate the expression of hTERT mRNA and protein, inhibiting telomerase activity and proliferation of endometrial cancer cell line in a dose- and period-dependent manner.


CONCLUSIONS
Antisense oligodeoxynucleotides of human telomerase transcriptase definitely inhibits the proliferation of endometrial cancer cell line. Telomerase inhibitor may thus become a new gene therapeutic agent for endometrial carcinoma.
Chen
Xue-jun
XJ
Medical College of Zhejiang University, HangZhou 310006, China.
Zheng
Wei
W
Wang
Sheng-qi
SQ
D020169:Caspases / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D016889:Endometrial Neoplasms / Q000473:pathology / Q000628:therapy*; D005260:Female; D015316:Genetic Therapy; D006801:Humans; D016376:Oligonucleotides, Antisense / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D019098:Telomerase / Q000037:antagonists & inhibitors* / Q000235:genetics
D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; C509186:TERT protein, human; D019098:Telomerase; D020169:Caspases