Expression and cellular localization of interleukin-6 mRNA in ovariectomized rats.
In order to observe the expression and cellular localization of interleukin-6 (IL-6) mRNA in bone tissue, ovaries of the rats were excised to develop osteoporosis model. The expression of IL-6 mRNA in bone tissues was detected by using dot blot hybridization assay and the cells producing IL-6 identified and localized by using in situ hybridization respectively. The results showed that the expression of IL-6 mRNA was significantly increased in the ovariectomized rats as compared with that in normal control rats and strong IL-6 mRNA hybridization signals were detected in lining cells, osteoblasts and osteocytes. It was suggested that loss of ovarian function induced in vivo osteoblast lineage increased IL-6 mRNA expression. IL-6 might play important roles in the development of bone loss following ovariectomy.
Tu
Y
Y
Department of Orthopedics, Xiehe Hospital, Tongji Medical University, Wuhan 430022.
Du
J
J
Yang
A
A
D000818:Animals; D001842:Bone and Bones / Q000378:metabolism; D005260:Female; D017403:In Situ Hybridization; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000235:genetics; D010006:Osteoblasts / Q000378:metabolism*; D010011:Osteocytes / Q000378:metabolism; D010024:Osteoporosis / Q000378:metabolism*; D010052:Ovariectomy; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley
D015850:Interleukin-6; D012333:RNA, Messenger