title: Serotype-specific detection of enterovirus 71 in clinical specimens by DNA microchip array.
abstract: Enterovirus 71 is an important pathogen that causes high morbidity and mortality in children in Taiwan. Virus isolation in cell cultures has been the standard method for enterovirus 71 identification in Clinical Virology Laboratories. However, virus isolation takes 5-10 days when using cell culture. A microchip for enterovirus 71 detection was developed as an alternative diagnostic method. The novel approach is based on hybridization of amplified DNA specimens with oligonucleotide DNA probes immobilized on a microchip. Two oligonucleotides were used as detection probes, the pan-enterovirus sequence located in the 5'-noncoding region (5'-NCR) and the enterovirus 71-specific sequence located in the VP2 region. The diagnostic procedure takes 6 h. One hundred specimens identified as enteroviruses by viral cultures were tested using this microchip, including 67 enterovirus 71 specimens. The sensitivity of the novel method is 89.6% and its specificity is 90.9%. The enterovirus 71-microchip can detect the amplicon derived from viral RNA corresponding to 1-10 virions in a clinical specimen. Microchip array is a potential diagnostic method for identification of enterovirus in the future.
authors.affiliation: School of Medical Technology, Chang Gung University, 259 Wen-Hua 1st Road, Kwei-Shan, Tao-Yuan 333, Taiwan, ROC. srshih@mail.cgu.edu.tw
authors.forename: Shin-Ru
authors.initials: SR
authors.lastname: Shih
authors.forename: Yih-Weng
authors.initials: YW
authors.lastname: Wang
authors.forename: Guang-Wu
authors.initials: GW
authors.lastname: Chen
authors.forename: Luan-Yin
authors.initials: LY
authors.lastname: Chang
authors.forename: Tzou-Yien
authors.initials: TY
authors.lastname: Lin
authors.forename: Mei-Chen
authors.initials: MC
authors.lastname: Tseng
authors.forename: Chiayn
authors.initials: C
authors.lastname: Chiang
authors.forename: Kuo-Chien
authors.initials: KC
authors.lastname: Tsao
authors.forename: Chung Guei
authors.initials: CG
authors.lastname: Huang
authors.forename: Mei-Ren
authors.initials: MR
authors.lastname: Shio
authors.forename: Jui-Hung
authors.initials: JH
authors.lastname: Tai
authors.forename: Shin-Hwan
authors.initials: SH
authors.lastname: Wang
authors.forename: Rei-Lin
authors.initials: RL
authors.lastname: Kuo
authors.forename: Wu-Tse
authors.initials: WT
authors.lastname: Liu
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics; D017931:DNA Primers; D018076:DNA, Complementary / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D004770:Enterovirus / Q000235:genetics / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000175:diagnosis; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012680:Sensitivity and Specificity; D012703:Serotyping; D014764:Viral Proteins / Q000235:genetics
chemical_list: D020121:5' Untranslated Regions; D017931:DNA Primers; D018076:DNA, Complementary; D004279:DNA, Viral; D014764:Viral Proteins