Novel mode of hormone induction of tandem tomato invertase genes in floral tissues.
The genomic organization of two extracellular invertase genes from tomato (Lin5 and Lin7), which are linked in a direct tandem repeat, and their tissue-specific and hormone-inducible expression are shown. Transient expression analysis of Lin5 promoter sequences fused to the beta-glucuronidase (GUS) reporter gene (uidA) demonstrates a specific expression of Lin5 during tomato fruit development. A Lin5 promoter fragment was fused to the truncated nos promoter to analyse hormone induction via GUS reporter gene activity in transiently transformed tobacco leaves. A specific up-regulation of GUS activity conferred by this Lin5 promoter fragment in response to gibberellic acid (GA), auxin and abscisic acid (ABA) treatment was observed, indicating a critical role of the regulation of Lin5 by phytohormones in tomato flower and fruit development. In situ hybridization analysis of Lin7 shows a high tissue-specific expression in tapetum and pollen. These results support an important role for Lin5 and Lin7 extracellular invertases in the development of reproductive organs in tomato and contribute to unravel the underlying regulatory mechanisms.
Proels
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Lehrstuhl für pharmazeutische Biologie, Universität Würzburg, Julius von Sachs Platz 2, 97082 Würzburg, Germany.
Hause
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B
Berger
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Roitsch
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D000040:Abscisic Acid / Q000494:pharmacology; D001483:Base Sequence; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D005091:Exons; D035264:Flowers / Q000201:enzymology / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005875:Gibberellins / Q000494:pharmacology; D006026:Glycoside Hydrolases / Q000235:genetics*; D017403:In Situ Hybridization; D007210:Indoleacetic Acids / Q000494:pharmacology; D007438:Introns; D007527:Isoenzymes / Q000235:genetics; D018551:Solanum lycopersicum / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D008969:Molecular Sequence Data; D010937:Plant Growth Regulators / Q000494:pharmacology*; D011401:Promoter Regions, Genetic / Q000235:genetics; D020218:Response Elements / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D016385:TATA Box / Q000235:genetics; D020080:Tandem Repeat Sequences / Q000235:genetics; D024363:Transcription Initiation Site; D043324:beta-Fructofuranosidase
D018744:DNA, Plant; D005875:Gibberellins; D007210:Indoleacetic Acids; D007527:Isoenzymes; D010937:Plant Growth Regulators; D000040:Abscisic Acid; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase