title: Molecular design of hairpin imidazole-pyrrole polyamide for effective DNA alkylation.
abstract: A new type of hairpin polyamide-CPI conjugate possessing a vinyl linker has been synthesized. Sequence-selective alkylation of double-stranded DNA by conjugates was investigated by high resolution denaturing gel electrophoresis using 450 bp DNA fragments. Highly-efficient DNA alkylation predominantly occurs at the purines of 5'-(A/T)G(T/A)CPu-3' site at nanomolar concentration.
authors.affiliation: CREST, Japan Science and Technology Corporation (JST), Japan.
authors.forename: T
authors.initials: T
authors.lastname: Bando
authors.forename: A
authors.initials: A
authors.lastname: Narita
authors.forename: I
authors.initials: I
authors.lastname: Saito
authors.forename: H
authors.initials: H
authors.lastname: Sugiyama
mesh_terms: D000478:Alkylation; D004247:DNA / Q000737:chemistry*; D007093:Imidazoles / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D009757:Nylons / Q000138:chemical synthesis* / Q000737:chemistry*; D011687:Purines / Q000737:chemistry; D011758:Pyrroles / Q000737:chemistry*
chemical_list: D007093:Imidazoles; D009757:Nylons; D011687:Purines; D011758:Pyrroles; D004247:DNA; C096654:N-methylpyrrole; C018100:1-methylimidazole