Production and characterization of biosurfactants from Bacillus licheniformis F2.2.
A biosurfactant-producing strain, Bacillus licheniformis F2.2, was isolated from a fermented food in Thailand. The strain was capable of producing a new biosurfactant, BL1193, as well as two kinds of popular lipopeptide biosurfactants, plipastatin and surfactin. Mass spectrometry and FT-IR analysis indicated that BL1193 had a molecular mass of 1,193 Da with no peptide portion in the molecule. While plipastatin and surfactin were abundantly produced in a nutrient YPD medium, BL1193 was produced only in a synthetic DF medium containing no amino acids. According to an oil displacement activity test, the specific activity of BL1193 (6.53 kBS units/mg) is equivalent to that of surfactin (5.78-6.83 kBS units/mg).
Thaniyavarn
Jiraporn
J
Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand. Jiraporn.Th@Chula.ac.th
Roongsawang
Niran
N
Kameyama
Takayuki
T
Haruki
Mitsuru
M
Imanaka
Tadayuki
T
Morikawa
Masaaki
M
Kanaya
Shigenori
S
D001407:Bacillus / Q000737:chemistry* / Q000254:growth & development; D005227:Fatty Acids; D005285:Fermentation; D005516:Food Microbiology; D055666:Lipopeptides; D008074:Lipoproteins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification*; D013058:Mass Spectrometry; D009842:Oligopeptides; D010456:Peptides, Cyclic; D017550:Spectroscopy, Fourier Transform Infrared; D013501:Surface-Active Agents / Q000032:analysis / Q000737:chemistry*
C069403:Bacillus licheniformis 86 surfactant; D005227:Fatty Acids; D055666:Lipopeptides; D008074:Lipoproteins; D009842:Oligopeptides; D010456:Peptides, Cyclic; D013501:Surface-Active Agents; C049901:plipastatin; C009365:surfactin peptide