Interaction of porphyrins bearing peripheral cationic heterocycles with G-quadruplex DNA.
Telomere-interactive compounds such as TMPyP4 are expected to inhibit telomerase and thus to be potential anti-cancer drugs. We investigated the interaction of porphyrins bearing peripheral cationic heterocycles with G-quadruplex DNA, which contained human telomeric repeats (GGGTTA)4. One of the porphyrins, pPyTTP, was found to bind to the G-quadruplex DNA and to increase the Tm of the G-quadruplex. The positive sign of induced CD for the complex suggested pPyTTP bound to and stabilized the G-quadruplex DNA possibly by groove binding, distinct from that of TMPyP4.
Ishikawa
Y
Y
Faculty of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Kumamoto 862-0973, Japan.
Yamashita
T
T
Tomisugi
Y
Y
Uno
T
T
D000970:Antineoplastic Agents / Q000737:chemistry* / Q000378:metabolism; D002412:Cations / Q000737:chemistry; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D054856:G-Quadruplexes; D006801:Humans; D046911:Macromolecular Substances; D009691:Nucleic Acid Denaturation; D011166:Porphyrins / Q000737:chemistry*; D012091:Repetitive Sequences, Nucleic Acid; D013053:Spectrophotometry; D016615:Telomere / Q000737:chemistry; D013696:Temperature
C476356:5,10,15,20-tetra(4-(1-pyridyl)phenyl)porphyrin; D000970:Antineoplastic Agents; D002412:Cations; D046911:Macromolecular Substances; D011166:Porphyrins; D004247:DNA