The effect of hyperthermia on DNA in mouse HepA cells--an analysis with computer and observation with EM.
A computer analysis system, automated imaging cytometry (AIC), flow cytometry (FCM) and EM, were used to study the relationship of apoptosis and the change of DNA in mouse HepA cells subjected to hyperthermia. Our results showed that pyknotic rate in the 44 degrees C group was significantly increased (P < 0.01) when compared with that in the 37 degrees C group, both the diploid rate and aneuploid rate in the 44 degrees C group were significantly decreased (P < 0.01) when compared with that in the 37 degrees C group. The pyknotic rate determined by ALC was in conformity with the apoptotic index (AI) determined by FCM. Under EM, ultrastructures of HapA cells appeared typical apoptosis changes in the 44 degrees C group. We are led to conclude that (1) when the temperature is 42 degrees C or 44 degrees C for 2 h, it did not promote the necrosis of HepA cells; (2) when the temperature is 42 degrees C for 2 h, it did not obviously induce the apoptosis of HepA cells, and the 44 degrees C for 2 h obviously induced the apoptosis of HepA cells; (3) AIC method can be used as an aid for the measurement of apoptotic index. Further study is needed to established it as a novel, simple and convenient method for determining the apoptosis.
Yu
D
D
Department of Anatomy, College of Basic Medical Sciences, Tongji Medical University, Wuhan 430030, China.
Liu
R
R
Zhou
J
J
Liu
Y
Y
D000818:Animals; D017209:Apoptosis; D002453:Cell Cycle; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry; D006358:Hot Temperature; D006979:Hyperthermia, Induced; D007091:Image Processing, Computer-Assisted; D008114:Liver Neoplasms, Experimental / Q000473:pathology* / Q000648:ultrastructure; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm