title: Effects of acetylsalicylic acid treatment on thyroid hormones, prolactins, and the stress response of tilapia (Oreochromis mossambicus).
abstract: The cyclooxygenase (COX) pathway converts arachidonic acid (ArA) into prostaglandins (PGs), which interact with the stress response in mammals and possibly in fish as well. Acetylsalicylic acid (ASA) is a COX inhibitor and was used to characterize the effects of PGs on the release of several hormones and the stress response of tilapia (Oreochromis mossambicus). Plasma PGE2 was significantly reduced at 100 mg ASA/kg body wt, and both basal PGE2 and cortisol levels correlated negatively with plasma salicylate. Basal plasma 3,5,3'-triiodothyronine (T3) was reduced by ASA treatment, whereas prolactin (PRL)188 increased at 100 mg ASA/kg body wt. ASA depressed the cortisol response to the mild stress of 5 min of net confinement. As expected, glucose and lactate were elevated in the stressed control fish, but the responses were blunted by ASA treatment. Gill Na+-K+-ATPase activity was not affected by ASA. Plasma osmolarity increased after confinement in all treatments, whereas sodium only increased at the high ASA dose. This is the first time ASA has been administered to fish in vivo, and the altered hormone release and the inhibition of the acute stress response indicated the involvement of PGs in these processes.
authors.affiliation: Dept. of Animal Ecology and Ecophysiology, Faculty of Science, Univ. of Nijmegen, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands. rvanholt@sci.kun.nl
authors.forename: Rogier D
authors.initials: RD
authors.lastname: van Anholt
authors.forename: Tom
authors.initials: T
authors.lastname: Spanings
authors.forename: William
authors.initials: W
authors.lastname: Koven
authors.forename: Sjoerd E
authors.initials: SE
authors.lastname: Wendelaar Bonga
mesh_terms: D000818:Animals; D001241:Aspirin / Q000494:pharmacology*; D001786:Blood Glucose; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology*; D005260:Female; D006854:Hydrocortisone / Q000097:blood; D019344:Lactic Acid / Q000097:blood; D008297:Male; D011388:Prolactin / Q000097:blood*; D012149:Restraint, Physical; D012964:Sodium / Q000097:blood; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013312:Stress, Physiological / Q000378:metabolism*; D013963:Thyroid Hormones / Q000097:blood*; D017210:Tilapia; D014882:Water-Electrolyte Balance / Q000187:drug effects
chemical_list: D001786:Blood Glucose; D016861:Cyclooxygenase Inhibitors; D013963:Thyroid Hormones; D019344:Lactic Acid; D011388:Prolactin; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D001241:Aspirin; D006854:Hydrocortisone