Murine hexose-6-phosphate dehydrogenase: a bifunctional enzyme with broad substrate specificity and 6-phosphogluconolactonase activity.
Murine hexose-6-phosphate dehydrogenase has been purified from liver microsomes by affinity chromatography on 2('),5(')-ADP-Sepharose. The purified enzyme has 6-phosphogluconolactonase activity and glucose-6-phosphate dehydrogenase activity and has a native molecular mass of 178 kDa and a subunit molecular mass of 89 kDa. Glucose 6-phosphate, galactose 6-phosphate, 2-deoxyglucose 6-phosphate, glucosamine 6-phosphate, and glucose 6-sulfate are substrates for murine hexose-6-phosphate dehydrogenase, with either NADP or deamino-NADP as coenzyme. This study confirms that hexose-6-phosphate dehydrogenase is a bifunctional enzyme which can catalyze the first two reactions of the pentose phosphate pathway.
Clarke
Julia L
JL
Department of Haematology, Faculty of Medicine, Imperial College London, Hammersmith Hospital, London W12 0NN, United Kingdom.
Mason
Philip J
PJ
D000595:Amino Acid Sequence; D000818:Animals; D002237:Carbohydrate Dehydrogenases / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry*; D003067:Coenzymes; D004789:Enzyme Activation; D005947:Glucose / Q000737:chemistry; D005958:Glucosephosphates / Q000737:chemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D008862:Microsomes, Liver / Q000737:chemistry* / Q000201:enzymology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009249:NADP / Q000737:chemistry; D010427:Pentose Phosphate Pathway; D013379:Substrate Specificity
D003067:Coenzymes; D005958:Glucosephosphates; C011085:glucose 6-(hydrogen sulfate); D009249:NADP; D002237:Carbohydrate Dehydrogenases; C020552:galactose-6-phosphate dehydrogenase; D002265:Carboxylic Ester Hydrolases; C022363:6-phosphogluconolactonase; D005947:Glucose