Insertion sequence-like elements associated with putative polyhydroxybutyrate regulatory genes in Azotobacter sp. FA8.
The genes phaR, phaP, and phaF, encoding putative regulatory proteins, were found in the poly (3-hydroxybutyrate) (PHB) gene cluster of the free nitrogen-fixing bacteria Azotobacter sp. FA8. These genes were flanked by the insertion sequence ISAzsp1, belonging to the IS3 family, and a region highly homologous to insertion sequences of the IS630 family. These are the first site-specific recombination elements to be described in association with genes involved in the metabolism of polyhydroxyalkanoates (PHAs). A possible role for ISs in the assembly of pha genes is presented.
Pettinari
Julia M
JM
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina.
Chaneton
Luciano
L
Vazquez
Gustavo
G
Steinbüchel
Alexander
A
Méndez
Beatriz S
BS
D020155:3-Hydroxybutyric Acid; D016948:Azotobacter vinelandii / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D005810:Multigene Family / Q000235:genetics; D009586:Nitrogen Fixation / Q000235:genetics*; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA
D004251:DNA Transposable Elements; D020155:3-Hydroxybutyric Acid