Study on the effect of ligustrazine on hematopoietic reconstitution in bone marrow transplantation mice.
To explore the effects of ligustrazine on hematopoietic reconstitution and its mechanism after bone marrow transplantation (BMT), the allogenic BMT mice were given intra-abdominal injection of 2 mg ligustrazine twice a day. On the 1st, 7th, 14th, and 28th day after BMT, peripheral blood cells and bone marrow nuclear cells (BMNC) were counted, and the histological features were evaluated. On the 7th, 14th, 21st day after BMT, CXCR4 expression on the BMNC was assayed. The results showed that peripheral blood cell counts and BMNC counts in ligustrazine-treated group on the 7th, 14th, 28th day were higher than those in BMT group (P < 0.01 or P < 0.05). The percentage of hematopoietic tissue volume, fat tissue hyperplasia and congestion and dilation degree of microvessel in ligustrazine-treated group on the 7th, 14th, 21st, 28th day was higher than those in BMT group. The CXCR4 expression levels in ligustrazine-treated group were higher than in BMT group (P < 0.01 or P < 0.05) on the 7th and 14th day, and were lower than in BMT group on the 21st day (P < 0.01). It is concluded that the ligustrazine can accelerate hematopoietic reconstruction, enhance growth of hematopoietic tissues and promote the repair of microvessels. The CXCR4 expression levels on BMNC may be responsible for the effect of ligustrazine.
Fang
M
M
Department of Internal Medicine, Tongji Hospital, Tongji Medical University, Wuhan 430030.
Sun
H
H
Liu
W
W
D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism; D016026:Bone Marrow Transplantation; D005260:Female; D006410:Hematopoiesis / Q000187:drug effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011719:Pyrazines / Q000494:pharmacology*; D011833:Radiation Injuries, Experimental / Q000188:drug therapy / Q000628:therapy; D019718:Receptors, CXCR4 / Q000096:biosynthesis; D014916:Whole-Body Irradiation
D011719:Pyrazines; D019718:Receptors, CXCR4; C017953:tetramethylpyrazine