title: Evaluation of the phytic acid effect on the labeling of blood elements with technetium-99m and on the survival of a strain of Escherichia coli treated with stannous fluoride.
abstract: The labeling of red blood cells with technetium-99m (99mTc) depends on a reducing agent and stannous ions, as chloride or fluoride, are widely utilized. This labeling may also be altered by drugs. Moreover, some authors have reported that the survival of Escherichia coli (E. coli) cultures decreases in presence of stannous ions. Phytic acid is present in the daily diet and we evaluated its influence on: (i) the labeling of blood elements with 99mTc and (ii) on the survival of an E. coli strain treated with stannous fluoride. Heparinized whole blood was withdrawn from Wistar rats and it was incubated with stannous chloride and with 99mTc, as sodium pertechnetate, centrifuged and plasma (P) and blood cells (BC) were isolated. Samples of P and BC were also precipitated with trichloroacetic acid, centrifuged and soluble (SF) and insoluble fractions (IF) isolated. E. coli culture was treated with stannous fluoride in presence of phytic acid. As phytic acid altered the fixation of 99mTc on BC, on IF-P and on IF-BC and, moreover, it abolished the lethal effect of stannous fluoride on the E. coli culture, we can suggest that, probably, phytic acid would have chelating properties to the stannous ions.
authors.affiliation: Departamento de Biofísica e Biometria, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brazil. gllf@hotmail.com
authors.forename: G L
authors.initials: GL
authors.lastname: Lima-Filho
authors.forename: G M T
authors.initials: GM
authors.lastname: Lima
authors.forename: R S
authors.initials: RS
authors.lastname: Freitas
authors.forename: L C M
authors.initials: LC
authors.lastname: Aleixo
authors.forename: S R F
authors.initials: SR
authors.lastname: Moreno
authors.forename: M T J A
authors.initials: MT
authors.lastname: Catanho
authors.forename: M
authors.initials: M
authors.lastname: Bernardo-Filho
mesh_terms: D000818:Animals; D001773:Blood Cells / Q000737:chemistry / Q000187:drug effects*; D004926:Escherichia coli / Q000187:drug effects*; D007553:Isotope Labeling / Q000379:methods*; D010833:Phytic Acid / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013667:Technetium / Q000097:blood* / Q000493:pharmacokinetics; D014002:Tin Fluorides / Q000494:pharmacology*
chemical_list: D014002:Tin Fluorides; D013667:Technetium; D010833:Phytic Acid