Chlamydia pneumoniae infection induces an unstable atherosclerotic plaque phenotype in LDL-receptor, ApoE double knockout mice.
OBJECTIVE
To study whether Chlamydia pneumoniae (Cpn) infection affects atherosclerotic plaque morphology in atherogenic (LDLr/ApoE(-/-)) mice.


METHODS
In mice sacrificed 20 or 40 weeks after Cpn infection aortic arch sections were analysed for lesion and fibrous cap area and the presence of matrix metalloproteinases (MMP)-2 and -9.


RESULTS
All infected mice seroconverted, demonstrated Cpn DNA in their aortas on PCR and developed atherosclerotic plaques. Infection was not associated with changes in lesion area or type, but was associated with reduced the fibrous cap area and increased MMP-2 and -9 immunoreactivity.


CONCLUSIONS
These findings suggest that Cpn infection may predispose to plaque instability.
Ezzahiri
R
R
Department of Vascular Surgery, University Hospital Maastricht, The Netherlands.
Stassen
F R M
FR
Kurvers
H A J M
HA
van Pul
M M L
MM
Kitslaar
P J E H M
PJ
Bruggeman
C A
CA
D000199:Actins / Q000032:analysis; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D001011:Aorta / Q000378:metabolism; D001018:Aortic Diseases / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D001057:Apolipoproteins E / Q000235:genetics; D001161:Arteriosclerosis / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology; D023521:Chlamydophila Infections / Q000150:complications*; D016993:Chlamydophila pneumoniae / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis; D007150:Immunohistochemistry; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000032:analysis; D020780:Matrix Metalloproteinase 9 / Q000032:analysis; D051379:Mice; D018345:Mice, Knockout; D010641:Phenotype; D011973:Receptors, LDL / Q000235:genetics
D000199:Actins; D000907:Antibodies, Bacterial; D001057:Apolipoproteins E; D004269:DNA, Bacterial; D011973:Receptors, LDL; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9