Analysis of anisotropic diamagnetic susceptibility of a bull sperm.
Bull sperm and paramecium cilium were exposed to uniform static magnetic fields to observe their magnetic orientations and measure their anisotropic diamagnetic susceptibility (deltachi) for each. The prepared samples were whole bull sperm, bull sperm flat heads, and paramecium cilia, because bull sperm tails in a perfect condition could not be prepared. The whole bull sperm and the bull sperm heads became oriented perpendicular to the magnetic fields (1.7 Tesla maximum), while the paramecium cilia became parallel to the magnetic fields (8 Tesla maximum). A whole bull sperm, a bull sperm head, and a paramecium cilium were photometrically studied to obtain deltachi for each, which were estimated to be 1 x 10(-19), 3 x 10(-19), and 2 x 10(-20) J/T(2), respectively. deltachi of a sperm flagellum was estimated from the measured value of deltachi of the paramecium cilium, which agrees well with the difference between deltachi of the whole sperm and the sperm head. Additionally, this difference of deltachi almost coincides with the deltachi values calculated from deltachi of tubulin, as well. If the magnetic effect on biological systems is solved and the magnetic orientation correlates with it, deltachi will become the quantitative index of the effect.
Emura
Runa
R
School of Allied Health Sciences, Faculty of Medicine, Osaka University, Suita, Osaka, Japan. emura@sahs.med.osaka-u.ac.jp
Takeuchi
Tetsuya
T
Nakaoka
Yasuo
Y
Higashi
Terumasa
T
D000818:Animals; D016880:Anisotropy; D002417:Cattle; D002465:Cell Movement / Q000502:physiology / Q000528:radiation effects*; D016764:Cell Polarity / Q000502:physiology / Q000528:radiation effects*; D002923:Cilia / Q000502:physiology / Q000528:radiation effects* / Q000648:ultrastructure; D004307:Dose-Response Relationship, Radiation; D004574:Electromagnetic Fields; D008297:Male; D010247:Paramecium / Q000166:cytology / Q000502:physiology / Q000528:radiation effects*; D010783:Photometry; D011829:Radiation Dosage; D013094:Spermatozoa / Q000166:cytology* / Q000502:physiology / Q000528:radiation effects*