Development of chemo-enzymatic process and manufacture of deoxynucleosides.
Manufacturing method for 2'-deoxynucleosides (dNus) has been developed using novel chemo-enzymatic process. The method consists of the chemical synthesis of 2'-deoxyribose 1-phosphate (dRP) and the enzymatic conversions of dRP into 2'-deoxyadenosine (dA), 2'-deoxyguanosine (dG) and 2'-deoxycytidine (dC). Large-scale preparation is successfully done, which demonstrates an applicability of this strategy to practical use. As thymidine is already under commercial-scale production, all four dNus are now ready for scale-up.
Komatsu
H
H
Chemical Synthesis Laboratory, Mitsui Chemicals Inc., 1144 Togo, Mobarashi, Chiba 297-0017, Japan.
Awano
H
H
Ishibashi
H
H
Ikeda
I
I
D003596:Cytosine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D003839:Deoxyadenosines / Q000096:biosynthesis; D003849:Deoxyguanosine / Q000096:biosynthesis; D003853:Deoxyribonucleosides / Q000096:biosynthesis* / Q000737:chemistry; D011683:Purine-Nucleoside Phosphorylase / Q000378:metabolism; D012267:Ribosemonophosphates / Q000138:chemical synthesis / Q000378:metabolism
D003839:Deoxyadenosines; D003853:Deoxyribonucleosides; D012267:Ribosemonophosphates; C031302:2-deoxyribose 1-phosphate; C085540:2'-deoxycytosine; D003596:Cytosine; D011683:Purine-Nucleoside Phosphorylase; D003849:Deoxyguanosine; C058118:2'-deoxyadenosine