Release of nitric oxide by N-nitropyrazoles in rabbit lacrimal gland cell culture.
OBJECTIVE
In order to prove the effects of N-nitropyrazoles to increase ocular blood flow and to facilitate retinal function after ischemia are due to nitric oxide (NO) release, eleven N-nitropyrazoles which are known NO donors were used to test their ability to produce NO in rabbit lacrimal gland (RLG) cell cultures.


METHODS
Nitropyrazoles (0.3-3 microg/ml) were added into RLG cell culture and the NO produced was determined with nitrate-nitrite colorimetric assay.


RESULTS
All nitropyrazoles (11 compounds) tested produced NO in various degrees with the highest degree by 13 fold over control (DN-5) and the lowest level by only 2.8 fold over control (DN-7).


CONCLUSIONS
The results indicated that NO is involved in ocular blood flow increase and retinal function recovery after ischemia by N-nitropyrazoles. However, there is no potency relationship between in vitro data and in vivo data, which is understandable because the experimental environments are different.
Xuan
Bo
B
Institute of Ocular Pharmacology and Department of Medical Pharmacology and Toxicology, Texas A & M University System Health Science Center, College Station 77843, USA.
Chiou
George C Y
GC
D000818:Animals; D002478:Cells, Cultured; D007765:Lacrimal Apparatus / Q000378:metabolism*; D009566:Nitrates / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism*; D020030:Nitric Oxide Donors / Q000494:pharmacology*; D009573:Nitrites / Q000378:metabolism; D009574:Nitro Compounds / Q000494:pharmacology*; D011720:Pyrazoles / Q000494:pharmacology*; D011817:Rabbits
D009566:Nitrates; D020030:Nitric Oxide Donors; D009573:Nitrites; D009574:Nitro Compounds; D011720:Pyrazoles; D009569:Nitric Oxide