The influence of pretreatment on cell stage progression and the time of DNA synthesis in barley ( Hordeum vulgare L) uninucleate microspores.
The objective of this study was to correlate the time that DNA synthesis first occurs in haploid microspores of barley with cell cycle and plant morphological stages and to subsequently assess the influence of pretreatments on DNA synthesis at different stages of microspore development. Spikes with microspores in early, mid, and late uninucleate stages of the two-rowed barley cultivars Manley and Igri were subjected to two commonly used pretreatments. First, during cold pretreatment for 28 days there was a slow increase in relative DNA values as well as asymmetric nuclear divisions in some microspores. Second, during a 4-day cold plus 0.3 M mannitol pretreatment, there was very little change in the microspore stage or DNA values indicating that for the duration of this pretreatment the progression of the cell cycle was essentially suspended at all stages, both in Igri and Manley. The results are discussed relative to the potential for genetic transformation of microspores.
Shim
Y S
YS
Department of Plant Agriculture, University of Guelph, N1G 2W1, Guelph, ON, Canada.
Kasha
K J
KJ
D002453:Cell Cycle / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects / Q000235:genetics; D003080:Cold Temperature; D004261:DNA Replication / Q000187:drug effects; D018744:DNA, Plant / Q000032:analysis / Q000096:biosynthesis*; D006238:Haploidy; D001467:Hordeum / Q000166:cytology* / Q000187:drug effects / Q000235:genetics*; D008353:Mannitol / Q000494:pharmacology; D014170:Transformation, Genetic
D018744:DNA, Plant; D008353:Mannitol