Glucose oxidation by Gluconobacter oxydans: characterization in shaking-flasks, scale-up and optimization of the pH profile.
In this study, the advantage of a novel measuring device for the online determination of oxygen and carbon dioxide transfer rates in shaking-flasks is reported for glucose oxidation by Gluconobacter oxydans. In this fermentation process, this device was used for the characterization of the oxidation pattern of different strains. G. oxydans NCIMB 8084 forms 2,5-diketogluconate from d-glucose in a multi-stage process via three different membrane-bound dehydrogenases. This strain was chosen for a scale-up of the process from shaking-flasks to a 2-l stirred vessel. An enhancement of 2,5-diketogluconate production was realized by controlling the pH at different levels during the fermentation.
Silberbach
M
M
Department of Biochemical Engineering, Aachen University of Technology, Worringer Weg 1, 52056 Aachen, Germany.
Maier
B
B
Zimmermann
M
M
Büchs
J
J
D001709:Biotechnology / Q000379:methods*; D002245:Carbon Dioxide / Q000378:metabolism; D003470:Culture Media; D005285:Fermentation; D005942:Gluconates / Q000378:metabolism; D020600:Gluconobacter oxydans / Q000254:growth & development / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D013997:Time Factors
D003470:Culture Media; D005942:Gluconates; D002245:Carbon Dioxide; C063875:2,5-diketogluconate; D005947:Glucose; D010100:Oxygen