title: Analysis of rat testicular protein expression following 91-day exposure to JP-8 jet fuel vapor.
abstract: We analyzed protein expression in preparations from whole testis in adult male Sprague-Dawley rats exposed for 6 h/d for 91 consecutive days to jet propulsion fuel-8 (JP-8) in the vapor phase (0, 250, 500, or 1000 mg/m(3) +/- 10%), simulating a range of possible human occupational exposures. Whole body inhalation exposures were carefully controlled to eliminate aerosol phase, and subjects were sacrificed within 48 h postexposure. Organ fractions were solubilized and separated via large-scale, high resolution two-dimensional electrophoresis, and gel patterns scanned, digitized and processed for statistical analysis. Seventy-six different testis proteins were significantly increased or decreased in abundance in vapor-exposed groups, compared to controls, and dose-response profiles were often nonlinear. A number of the proteins were identified by peptide mass fingerprinting and related to histopathological or physiological deficits shown in previously published studies to occur with repeated exposure to hydrocarbon fuels or solvents. These results demonstrate a significant effect of JP-8 exposure on protein expression, particularly in protein expression in the rodent testis, and suggest that a 91 d exposure to jet fuel vapor induces changes of equal or greater magnitude to those reported previously for shorter duration JP-8 aerosol exposures.
authors.affiliation: Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, IN 46202, USA. fwitzman@iupui.edu
authors.forename: Frank A
authors.initials: FA
authors.lastname: Witzmann
authors.forename: Andrew
authors.initials: A
authors.lastname: Bobb
authors.forename: G Bruce
authors.initials: GB
authors.lastname: Briggs
authors.forename: Heather N
authors.initials: HN
authors.lastname: Coppage
authors.forename: Rex A
authors.initials: RA
authors.lastname: Hess
authors.forename: Junyu
authors.initials: J
authors.lastname: Li
authors.forename: Nathan M
authors.initials: NM
authors.lastname: Pedrick
authors.forename: Glenn D
authors.initials: GD
authors.lastname: Ritchie
authors.forename: John
authors.initials: J
authors.lastname: Rossi
authors.forename: Kenneth R
authors.initials: KR
authors.lastname: Still
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D006838:Hydrocarbons / Q000633:toxicity*; D019570:Inhalation Exposure / Q000009:adverse effects*; D007648:Kerosene / Q000633:toxicity*; D008297:Male; D010449:Peptide Mapping; D011506:Proteins / Q000032:analysis* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013737:Testis / Q000737:chemistry*; D036401:Toxicity Tests, Chronic
chemical_list: D006838:Hydrocarbons; C098607:JP8 aviation fuel; D007648:Kerosene; D011506:Proteins