title: A functional genomics approach toward the understanding of secondary metabolism in plant cells.
abstract: Despite the tremendous importance of secondary metabolites for humans as for the plant itself, plant secondary metabolism remains poorly characterized. Here, we present an experimental approach, based on functional genomics, to facilitate gene discovery in plant secondary metabolism. Targeted metabolite analysis was combined with cDNA-amplified fragment length polymorphism-based transcript profiling of jasmonate-elicited tobacco Bright yellow 2 cells. Transcriptome analysis suggested an extensive jasmonate-mediated genetic reprogramming of metabolism, which correlated well with the observed shifts in the biosynthesis of the metabolites investigated. This method, which in addition to transcriptome data also generates gene tags, in the future might lead to the creation of novel tools for metabolic engineering of medicinal plant systems in general.
authors.affiliation: Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
authors.forename: Alain
authors.initials: A
authors.lastname: Goossens
authors.forename: Suvi T
authors.initials: ST
authors.lastname: Häkkinen
authors.forename: Into
authors.initials: I
authors.lastname: Laakso
authors.forename: Tuulikki
authors.initials: T
authors.lastname: Seppänen-Laakso
authors.forename: Stefania
authors.initials: S
authors.lastname: Biondi
authors.forename: Valerie
authors.initials: V
authors.lastname: De Sutter
authors.forename: Freya
authors.initials: F
authors.lastname: Lammertyn
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Nuutila
authors.forename: Hans
authors.initials: H
authors.lastname: Söderlund
authors.forename: Marc
authors.initials: M
authors.lastname: Zabeau
authors.forename: Dirk
authors.initials: D
authors.lastname: Inzé
authors.forename: Kirsi-Marja
authors.initials: KM
authors.lastname: Oksman-Caldentey
mesh_terms: D000085:Acetates / Q000494:pharmacology; D000470:Alkaloids / Q000096:biosynthesis; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D003517:Cyclopentanes / Q000494:pharmacology; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D023281:Genomics; D009538:Nicotine / Q000096:biosynthesis; D020411:Oligonucleotide Array Sequence Analysis; D054883:Oxylipins; D011073:Polyamines / Q000378:metabolism; D014026:Nicotiana / Q000166:cytology / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D000085:Acetates; D000470:Alkaloids; D003517:Cyclopentanes; D018076:DNA, Complementary; D018744:DNA, Plant; D054883:Oxylipins; D011073:Polyamines; D009538:Nicotine; C072239:methyl jasmonate