title: Isolation and initial characterization of temperature-sensitive mutants of Arabidopsis thaliana that are impaired in root redifferentiation.
abstract: As tools for studying adventitious organogenesis, three temperature-sensitive mutants of Arabidopsis thaliana, designated rrd1, rrd2, and rrd4, were isolated by screening with callus-mediated root redifferentiation from hypocotyl explants as an index phenotype. Phenotypes of these mutants were characterized with special regard to their tissue-culture responses. The obtained results suggest that the RRD1 and RRD2 genes participate in some fundamental processes required for active cell proliferation and that the RRD4 gene is involved in the acquisition step of competence for cell proliferation during callus initiation in hypocotyl explants.
authors.affiliation: Botanical Gardens, Graduate School of Science, The University of Tokyo, Hakusan 3-7-1, Bunkyo-ku, Tokyo, 112-0001 Japan. sugiyama@ns.bg.s-tokyo.ac.jp
authors.forename: Munetaka
authors.initials: M
authors.lastname: Sugiyama
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics*; D002874:Chromosome Mapping; D004252:DNA Mutational Analysis; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics; D009154:Mutation / Q000235:genetics*; D018517:Plant Roots / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D011995:Recombination, Genetic / Q000235:genetics; D036226:Seedlings / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D013696:Temperature