title: Glutamate counteracts the denaturing effect of urea through its effect on the denatured state.
abstract: The urea induced equilibrium denaturation behavior of glutaminyl-tRNA synthetase from Escherichia coli (GlnRS) in 0.25 m potassium l-glutamate, a naturally occurring osmolyte in E. coli, has been studied. Both the native to molten globule and molten globule to unfolded state transitions are shifted significantly toward higher urea concentrations in the presence of l-glutamate, suggesting that l-glutamate has the ability to counteract the denaturing effect of urea. d-Glutamate has a similar effect on the equilibrium denaturation of glutaminyl-tRNA synthetase, indicating that the effect of l-glutamate may not be due to substrate-like binding to the native state. The activation energy of unfolding is not significantly affected in the presence of 0.25 m potassium l-glutamate, indicating that the native state is not preferentially stabilized by the osmolyte. Dramatic increase of coefficient of urea concentration dependence (m) values of both the transitions in the presence of glutamate suggests destabilization and increased solvent exposure of the denatured states. Four other osmolytes, sorbitol, trimethylamine oxide, inositol, and triethylene glycol, show either a modest effect or no effect on native to molten globule transition of glutaminyl-tRNA synthetase. However, glycine betaine significantly shifts the transition to higher urea concentrations. The effect of these osmolytes on other proteins is mixed. For example, glycine betaine counteracts urea denaturation of tubulin but promotes denaturation of S228N lambda-repressor and carbonic anhydrase. Osmolyte counteraction of urea denaturation depends on osmolyte-protein pair.
authors.affiliation: Department of Biophysics, Bose Institute, P-1/12 C. I. T. Scheme VII M, Calcutta 700 054, India.
authors.forename: Amit Kumar
authors.initials: AK
authors.lastname: Mandal
authors.forename: Soma
authors.initials: S
authors.lastname: Samaddar
authors.forename: Rajat
authors.initials: R
authors.lastname: Banerjee
authors.forename: Simanti
authors.initials: S
authors.lastname: Lahiri
authors.forename: Anusree
authors.initials: A
authors.lastname: Bhattacharyya
authors.forename: Siddhartha
authors.initials: S
authors.lastname: Roy
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000737:chemistry; D000818:Animals; D001622:Betaine / Q000494:pharmacology; D002256:Carbonic Anhydrases / Q000737:chemistry; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000201:enzymology; D005971:Glutamates / Q000494:pharmacology; D018698:Glutamic Acid / Q000737:chemistry*; D007294:Inositol / Q000494:pharmacology; D007700:Kinetics; D008744:Methylamines / Q000494:pharmacology; D016877:Oxidants / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D011485:Protein Binding; D011487:Protein Conformation; D011489:Protein Denaturation; D017510:Protein Folding; D013012:Sorbitol / Q000494:pharmacology; D013053:Spectrophotometry; D013696:Temperature; D013816:Thermodynamics; D013997:Time Factors; D014357:Trypsin / Q000494:pharmacology; D014404:Tubulin / Q000737:chemistry; D014508:Urea / Q000494:pharmacology*
chemical_list: D005971:Glutamates; D008744:Methylamines; D016877:Oxidants; D014404:Tubulin; D018698:Glutamic Acid; D001622:Betaine; D007294:Inositol; D013012:Sorbitol; D011189:Potassium Chloride; D014508:Urea; D014357:Trypsin; D002256:Carbonic Anhydrases; D000604:Amino Acyl-tRNA Synthetases; C020659:glutaminyl-tRNA synthetase; C005855:trimethyloxamine