title: Structural and serological studies on a new 4-deoxy-d-arabino-hexose-containing O-specific polysaccharide from the lipopolysaccharide of Citrobacter braakii PCM 1531 (serogroup O6).
abstract: The O-specific polysaccharide of Citrobacter braakii PCM 1531 (serogroup O6) was isolated by mild acid hydrolysis of the lipopolysaccharide (LPS) and found to contain d-fucose, l-rhamnose, 4-deoxy-d-arabino-hexose and O-acetyl groups in molar ratios 2 : 1 : 1 : 1. On the basis of methylation analysis and 1H and 13C NMR spectroscopy data, the structure of the branched tetrasaccharide repeating unit of the O-specific polysaccharide was established. Using various serological assays, it was demonstrated that the LPS of strain PCM 1531 is not related serologically to other known 4-deoxy-d-arabino-hexose-containing LPS from Citrobacter PCM 1487 (serogroup O5) or C. youngae PCM 1488 (serogroup O36). Two other strains of Citrobacter, PCM 1504 and PCM 1505, which, together with strain PCM 1531, have been classified in serogroup O6, were shown to be serologically distinct from strain PCM 1531 and should be reclassified into another serogroup.
authors.affiliation: L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland. katzenel@immuno.iitd.pan.wroc.pl
authors.forename: Ewa
authors.initials: E
authors.lastname: Katzenellenbogen
authors.forename: Nina A
authors.initials: NA
authors.lastname: Kocharova
authors.forename: George V
authors.initials: GV
authors.lastname: Zatonsky
authors.forename: Danuta
authors.initials: D
authors.lastname: Witkowska
authors.forename: Maria
authors.initials: M
authors.lastname: Bogulska
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: Andrzej
authors.initials: A
authors.lastname: Gamian
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D002954:Citrobacter / Q000145:classification / Q000276:immunology*; D003837:Deoxy Sugars / Q000032:analysis*; D005779:Immunodiffusion; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000378:metabolism; D008745:Methylation; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D019081:O Antigens / Q000737:chemistry* / Q000276:immunology*; D012703:Serotyping
chemical_list: D003837:Deoxy Sugars; D008070:Lipopolysaccharides; D019081:O Antigens