Antigen processing of the heptavalent pneumococcal conjugate vaccine carrier protein CRM(197) differs depending on the serotype of the attached polysaccharide.
The pneumococcal (Pn) conjugate vaccine includes seven different polysaccharides (PS) conjugated to CRM(197). Utilizing antigen-processing cells and a CRM(197)-specific mouse T-cell hybridoma, we found that the serotype of conjugated PnPS dramatically affected antigen processing of CRM(197). Unconjugated CRM(197) and serotype conjugates 14 and 18C were processed more efficiently.
Leonard
Ethan G
EG
Department of Pediatrics, Case Western Reserve University, Cleveland, Ohio 44106, USA.
Canaday
David H
DH
Harding
Clifford V
CV
Schreiber
John R
JR
D000595:Amino Acid Sequence; D000818:Animals; D017951:Antigen Presentation; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000378:metabolism*; D015798:HLA-DR1 Antigen / Q000502:physiology; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D022242:Pneumococcal Vaccines / Q000008:administration & dosage*; D011135:Polysaccharides, Bacterial / Q000008:administration & dosage*; D012703:Serotyping; D013601:T-Lymphocytes / Q000276:immunology; D018074:Vaccines, Conjugate / Q000008:administration & dosage*
D001426:Bacterial Proteins; D015798:HLA-DR1 Antigen; D022242:Pneumococcal Vaccines; D011135:Polysaccharides, Bacterial; D018074:Vaccines, Conjugate; C028581:CRM197 (non-toxic variant of diphtheria toxin)