Genistein protects primary cortical neurons from iron-induced lipid peroxidation.
Neuroprotective role of isoflavones in particular genistein might be resulted from their antioxidant activities in addition to their estrogenic actions. In the present study, we investigated effect of genistein on iron-induced free radical reaction in cultured cortical neurons. Thiobarbituric-acid-reactive species (TBARS) and superoxide dismutase (SOD) were measured after incubation of the cells with different concentrations of genistein in the absence or presence of iron (12.5 microM) for 24 h. Genistein at 100 microM significantly reduced the iron-induced TBARS, implying that genistein has an inhibitory role on iron-induced lipid peroxidation. Also, genistein (100 microM) led to a relatively higher SOD level than that in iron treatment although no significant difference was found. The findings imply that the antioxidative effect of genistein is partly associated with its neuroprotective function.
Ho
Kwok Ping
KP
Laboratory of Brain Iron Metabolism, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Kowloon, Hong Kong, Peoples Republic of China. bczmqian@polyu.edu.hk
Li
Lin
L
Zhao
Li
L
Qian
Zhong Ming
ZM
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005609:Free Radicals / Q000378:metabolism; D019833:Genistein / Q000494:pharmacology*; D007501:Iron / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000187:drug effects / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000975:Antioxidants; D005609:Free Radicals; D017392:Thiobarbituric Acid Reactive Substances; D019833:Genistein; D007501:Iron; D013482:Superoxide Dismutase