CA125 and UQCRFS1 FISH studies of ovarian carcinoma.
OBJECTIVE
With the gene CA125 having recently been cloned, we chose to investigate the gene copy number of various ovarian cancer samples by FISH. As a control we chose BACs close to the chromosome 19 centromere. One of these BACs carries the gene UQCRFS1.


METHODS
We developed FISH probes for CA125 and the UQCRFS1 region. We studied 22 touch preparations and 14 paraffin-embedded samples of ovarian carcinomas with known CA125 serum levels, two ovarian cancer cell lines, and one ascites sample from an ovarian cancer patient. The average copy number per cell of both probes was calculated. Metaphase analyses were done on cell lines and ascites cells to localize the signals.


RESULTS
The CA125 gene mapped to 19p13.2. Three of 22 (13.6%) touch preparations and 1 of 14 (7.1%) paraffin samples had amplified levels of CA125. The cell lines and ascites sample did not have amplified CA125. Unexpectedly, 3 of 22 (13.6%) touch preparations, 1 of 14 (7.1%) paraffin samples, one cell line, and the ascites sample had amplification of the UQCRFS1 region. The amplification of the UQCRFS1 region occurred in the form of homogeneously staining regions (HSRs). Only one sample had coamplification of CA125 and UQCRFS1.


CONCLUSIONS
CA125 was only sometimes modestly amplified in ovarian carcinoma, even when the serum CA125 level was highly elevated. Unexpectedly, the UQCRFS1 region was also sometimes amplified as HSRs. The UQCRFS1 protein is also known as complex III of the mitochondrial respiratory chain. This product may have an important role in malignant cells.
Kaneko
Saori J
SJ
Department of Obstetrics and Gynecology, University of South Carolina School of Medicine, Columbia, SC 29203, USA.
Gerasimova
Tsilya
T
Smith
S Terrell
ST
Lloyd
Kenneth O
KO
Suzumori
Kaoru
K
Young
S Robert
SR
D018394:CA-125 Antigen / Q000097:blood / Q000235:genetics*; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics; D014450:Electron Transport Complex III / Q000235:genetics*; D005260:Female; D018628:Gene Dosage; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007506:Iron-Sulfur Proteins / Q000235:genetics*; D010051:Ovarian Neoplasms / Q000097:blood / Q000235:genetics*; D016612:Paraffin Embedding; D014407:Tumor Cells, Cultured
D018394:CA-125 Antigen; D007506:Iron-Sulfur Proteins; C037989:Rieske iron-sulfur protein; D014450:Electron Transport Complex III