Oral Candida albicans and Candida dubliniensis differentiation by multilocus enzyme electrophoresis and sodium dodecylsulphate-polyacrylamide gel electrophoresis.
Five oral strains of Candida albicans and five C. dubliniensis, as well as their respective type-strains, were analyzed by multilocus enzyme electrophoresis (MLEE) and sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE). After electrophoreses and numerical analyses, we obtained two distinct species-specific taxa, which may justify the use of MLEE and SDS-PAGE as reliable methods for differentiation and complementary identification of C. dubliniensis.
Rosa
E A
EA
Stomatology Laboratory, Center of Biological and Health Sciences, Pontifical Catholic University of Paraná, Avenida Imaculada Conceição, 1155-Prado Velho-CEP 80215-901, Curitiba, Brazil. earrosa@rla01.pucpr.br
Rosa
R T
RT
Boriollo
M F
MF
Bernardo
W L
WL
Höfling
J F
JF
D000426:Alcohol Dehydrogenase / Q000032:analysis; D002175:Candida / Q000145:classification* / Q000201:enzymology / Q000302:isolation & purification; D002176:Candida albicans / Q000201:enzymology / Q000302:isolation & purification; D002180:Candidiasis, Oral / Q000382:microbiology*; D002374:Catalase / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005656:Fungal Proteins / Q000032:analysis*; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D006801:Humans; D007521:Isocitrate Dehydrogenase / Q000032:analysis; D007527:Isoenzymes / Q000032:analysis*; D007931:Leucyl Aminopeptidase / Q000032:analysis; D009055:Mouth / Q000382:microbiology*; D009172:Mycology / Q000379:methods*; D009195:Peroxidase / Q000032:analysis; D012015:Reference Standards; D012967:Sodium Dodecyl Sulfate; D013045:Species Specificity; D013482:Superoxide Dismutase / Q000032:analysis
D005656:Fungal Proteins; D007527:Isoenzymes; D012967:Sodium Dodecyl Sulfate; D000426:Alcohol Dehydrogenase; D007521:Isocitrate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase; D007931:Leucyl Aminopeptidase