title: X-ray analyses of d(GCGAAAGC) and d(GXGAAAGCT), where X = 2'-deoxy-5-iodocytidine.
abstract: DNA fragments containing a sequence d(GCGAAAGC) are known to be highly thermostable. To investigate the structural basis for such a specific property, crystallographic studies of the DNA octamer and a nanomer d(GXGAAAGCT) (X = 2'-deoxy-5-iodocytidine) have been performed. The present higher resolution X-ray analyses have shown that both DNA oligomers are stabilized respectively to form a zipper-type duplex homodimer. PAGE of these oligomers, however, indicates that they are monomeric even when their crystals were dissolved at room temperature.
authors.affiliation: Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori-ku, Yokohama 226-8501, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sunami
authors.forename: J
authors.initials: J
authors.lastname: Kondo
authors.forename: T
authors.initials: T
authors.lastname: Chatake
authors.forename: I
authors.initials: I
authors.lastname: Hirao
authors.forename: K
authors.initials: K
authors.lastname: Watanabe
authors.forename: K
authors.initials: K
authors.lastname: Miura
authors.forename: A
authors.initials: A
authors.lastname: Takénaka
mesh_terms: D001483:Base Sequence; D001972:Bromodeoxycytidine / Q000031:analogs & derivatives; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry*; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000737:chemistry*; D019281:Dimerization; D008958:Models, Molecular; D009690:Nucleic Acid Conformation
chemical_list: D003841:Deoxycytidine; D001972:Bromodeoxycytidine; C011288:ibacitabine; D004247:DNA