[Effects of "Qiang Gu Kang Wei" compound prescription on biochemical indices of bone and related organs in rats under simulated weightlessness].
OBJECTIVE
To investigate effects of "Qiang Gu Kang Wei" prescription on biochemical indices of bone and related organs in tail-suspended rats.


METHODS
Rats were tail-suspended (-30 degrees) for 21 d to simulate weightlessness. The effect of this prescription on biochemical indices such as Ca, P in serum, osteocalcin (BGP) in serum and bone, oxyproline hydroxyproline (Hyp) in bone, alkaliphosphatase (ALP) in serum, small intestine and bone in these rats were examined.


RESULTS
Comparing with the normal control, serum Ca decreased significantly (P<0.05) while serum P showed only a trend to decrease, at the same time BGP dropped in both serum and bone (P<0.05) and Hyp dropped only in bone (P<0.05). There was significant decrease of ALP activity in serum, small intestine and bone (P<0.01 or P<0.05) in suspended rats. The prescription showed different effects with different doses. In all the three dose groups (30 g/kg, 20 g/kg, 10 g/kg) Ca and P in serum levels increased (P<0.01 or P<0.05), BGP in serum and bone improved (P<0.05) or showed only a trend to increase. ALP activity increased in serum, small intestine and bone (P<0.05). Increase of Hyp in bone was found in the middle and the small dose groups (P<0.05).


CONCLUSIONS
This prescription shows an effect of improving biochemical indices in bone and related organs such as serum and small intestine in tail-suspended rats. But no distinct concentration-dependence was found.
Li
Yong-zhi
YZ
Institute of Space Medico-Engineering, Beijing, China.
Shi
Hong-zhi
HZ
Fan
Quan-chun
QC
Bai
Gui-e
GE
Duan
Yuan-ru
YR
Xie
Jun-shui
JS
D000469:Alkaline Phosphatase / Q000097:blood / Q000378:metabolism; D000818:Animals; D001842:Bone and Bones / Q000187:drug effects* / Q000378:metabolism*; D002118:Calcium / Q000097:blood / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D019417:Hindlimb Suspension / Q000009:adverse effects; D006909:Hydroxyproline / Q000097:blood / Q000378:metabolism; D007421:Intestine, Small / Q000187:drug effects* / Q000378:metabolism*; D015675:Osteocalcin / Q000097:blood / Q000378:metabolism; D010758:Phosphorus / Q000097:blood / Q000378:metabolism; D051381:Rats; D018474:Weightlessness Simulation / Q000009:adverse effects*
D004365:Drugs, Chinese Herbal; D015675:Osteocalcin; D010758:Phosphorus; D000469:Alkaline Phosphatase; D006909:Hydroxyproline; D002118:Calcium