[Effects of the composition of bone tissue growth factors on cell growth on the model of long term culture of the bone marrow].
The experimental study of the properties of the collagen- and hydroxyapatite-containing osteoplastic material HAPKOL-I by bone marrow hemopoiesis was made in comparison with existing material HAPKOL. HAPKOL-I is a working name of the material consisting of HAPKOL and composition of non-collagen bone proteins produced from xenogenic bone material. Long-term cultures of bone marrow from mice (C57B1/6xCBA)F1 of dexter type were used. Cultivation time was 7, 14, 21 and 28 days. Intensity of hemopoietic processes was assessed on the samples; absolute count of stromal and hemopoietic cells on the membrane was calculated. It was found that in long-term cultures of the bone marrow stromal and hemopoietic cells develop on the materials HAPKOL and HAPKOL-I, processes of formation of hemopoietic microenvironment provide normal hemopoiesis. On both materials there was a gradual increase of cell counts, a rise in the number of stromal and a fall in the number of hemopoietic cells. More intensive hemopoiesis on the material containing non-collagen bone proteins may be due to this factor.
Volozhin
A I
AI
Bondarenko
O A
OA
Desiatnichenko
K S
KS
Kurdiumov
S G
SG
Lebedev
V G
VG
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects*; D001854:Bone Marrow Cells / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003094:Collagen / Q000737:chemistry / Q000494:pharmacology*; D046508:Culture Techniques / Q000379:methods*; D004338:Drug Combinations; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology*; D006133:Growth Substances / Q000737:chemistry* / Q000494:pharmacology*; D008422:Materials Testing; D051379:Mice; D008815:Mice, Inbred Strains; D017154:Stromal Cells / Q000187:drug effects; D013997:Time Factors
D004338:Drug Combinations; D006133:Growth Substances; C104010:Hapcol; D003094:Collagen; D017886:Durapatite