title: The alpha-L-ribo-isomers of RNA and LNA (locked nucleic acid).
abstract: The phosphoramidite approach has been used for the automated synthesis of alpha-L-LNA, alpha-L-RNA, and oligomers composed of mixtures of alpha-L-LNA, alpha-L-RNA and DNA monomers. Binding studies revealed very efficient recognition of single-stranded DNA and RNA target oligonucleotide strands. alpha-L-LNAs were shown to be significantly stabilized towards 3'-exonucleolytic degradation. Duplexes formed between RNA and alpha-L-LNA induced E. coli RNase H-mediated RNA cleavage, albeit very slow, at high enzyme concentration.
authors.affiliation: Department of Chemistry, University of Copenhagen, Universitetsparken 5, DK-2100 Copenhagen, Denmark.
authors.forename: M D
authors.initials: MD
authors.lastname: Sørensen
authors.forename: L
authors.initials: L
authors.lastname: Kvaernø
authors.forename: T
authors.initials: T
authors.lastname: Bryld
authors.forename: A E
authors.initials: AE
authors.lastname: Håkansson
authors.forename: L
authors.initials: L
authors.lastname: Keinicke
authors.forename: B
authors.initials: B
authors.lastname: Verbeure
authors.forename: P
authors.initials: P
authors.lastname: Herdewijn
authors.forename: J
authors.initials: J
authors.lastname: Wengel
mesh_terms: D004247:DNA / Q000737:chemistry; D004722:Endoribonucleases / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D005092:Exonucleases / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D009696:Nucleic Acids / Q000737:chemistry* / Q000378:metabolism; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D013237:Stereoisomerism
chemical_list: D009696:Nucleic Acids; D012313:RNA; D004247:DNA; D004722:Endoribonucleases; D005092:Exonucleases