title: Analysis of the variation in the hsp70-1 and hsp90alpha mRNA expression in human myocardial tissue that has undergone surgical stress.
abstract: In the present work we reported a semiquantitative detection of messenger ribonucleic acids (mRNAs) encoding the human heat shock proteins Hsp70-1, the stress inducible member of the HSP70 family, and hsp90alpha, the inducible member of the HSP90 family. We investigated the change in the expression of these mRNAs in tissue samples taken from the right atrium of 48 pediatric patients, soon after the ischemic period during surgery to correct congenital heart diseases, in which a crystalloid cold cardioplegic solution was used. No significant variations were found for either hsp70-1 or hsp90alpha expressions. Moreover, we searched for an association between the hsp70-1 promoter region polymorphism and the expression of the hsp70-1 in a smaller group of these patients (n = 27). The -110AA genotype was on average significantly associated with a decrease in the hsp70-1 mRNA level (P < 0.05), whereas the other genotypes -110AC or -110CC did not seem to be associated with the hsp70-1 expression level. The lack of any observed increase in the hsp70-1 expression level may be due to the high basal level of the Hsp70 protein in the tissues examined.
authors.affiliation: Clinical Physiology Institute, G. Pasquinucci Hospital, National Research Council, Massa, Italy. kingle@ifc.pi.cnr.it
authors.forename: Simona
authors.initials: S
authors.lastname: Storti
authors.forename: Simona
authors.initials: S
authors.lastname: Vittorini
authors.forename: Maria Rosaria
authors.initials: MR
authors.lastname: Iascone
authors.forename: Monica
authors.initials: M
authors.lastname: Sacchelli
authors.forename: Alessandra
authors.initials: A
authors.lastname: Baroni
authors.forename: Vincenzo Stefano
authors.initials: VS
authors.lastname: Luisi
authors.forename: Adrian
authors.initials: A
authors.lastname: Crucean
authors.forename: Vittorio
authors.initials: V
authors.lastname: Vanini
authors.forename: Andrea
authors.initials: A
authors.lastname: Biagini
authors.forename: Aldo
authors.initials: A
authors.lastname: Clerico
mesh_terms: D000293:Adolescent; D000375:Aging / Q000502:physiology; D006348:Cardiac Surgical Procedures; D002315:Cardiopulmonary Bypass; D002648:Child; D002675:Child, Preschool; D005260:Female; D005838:Genotype; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D018841:HSP90 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D006325:Heart Atria / Q000378:metabolism / Q000473:pathology; D006330:Heart Defects, Congenital / Q000601:surgery; D006801:Humans; D007223:Infant; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D011110:Polymorphism, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D012333:RNA, Messenger; C081884:heat-shock protein 70.1