title: Proline reduces creatine kinase activity in the brain cortex of rats.
abstract: Type II hyperprolinemia is an inherited disorder caused by a deficiency of delta 1-pyrroline-5-carboxilic acid dehydrogenase, whose biochemical hallmark is proline accumulation in plasma and tissues. Although neurological symptoms occur in most patients, the neurotoxicity of proline is still controversial. The main objective of the present study was to investigate the effect of acute and chronic administration of proline on creatine kinase activity of brain cortex of Wistar rats. Acute treatment was performed by subcutaneous administration of one injection of proline to 22-day-old rats. For chronic treatment, proline was administered twice a day from the 6th to the 21st postpartum day. The results showed that creatine kinase activity was significantly inhibited in the brain cortex of rats subjected to acute proline administration. In contrast, this activity was increased in animals subjected to chronic administration. We also measured the in vitro effect of proline on creatine kinase activity in cerebral cortex of 22-day-old nontreated rats. Proline significantly inhibited creatine kinase activity. Considering the importance of creatine kinase forthe maintenance of energy homeostasis in the brain, it is conceivable that an alteration of this enzyme activity in the brain may be one of the mechanisms by which proline might be neurotoxic.
authors.affiliation: Departamento de Bioquímica, Instituto de Ciências Básicas de Saúde, UFRGS, Porto Alegre, RS, Brazil.
authors.forename: Adriana
authors.initials: A
authors.lastname: Kessler
authors.forename: Elisa
authors.initials: E
authors.lastname: Costabeber
authors.forename: Carlos Severo
authors.initials: CS
authors.lastname: Dutra-Filho
authors.forename: Angela Terezinha Souza
authors.initials: AT
authors.lastname: Wyse
authors.forename: Moacir
authors.initials: M
authors.lastname: Wajner
authors.forename: Clóvis Milton Duval
authors.initials: CM
authors.lastname: Wannmacher
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects* / Q000201:enzymology; D003402:Creatine Kinase / Q000378:metabolism*; D003600:Cytosol / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D011392:Proline / Q000494:pharmacology*; D051381:Rats
chemical_list: D011392:Proline; D003402:Creatine Kinase