Comparison of isotopic fractionation in lactic acid and ethanol fermentations.
Pure D(-) and L(+) enantiomers of lactic acid were prepared by fermentation reactions with specific bacteria. In addition, naturally deuterated ethanol was prepared and converted into diastereoisomers using mandelic acid. Various sugars and nutrients were fermented into lactic acid in water having different deuterium contents and ethanol samples were obtained from yeast fermentation of sugars from different botanical origins. The methine and methylene groups in lactic acid and ethanol respectively show similar deuterium contents which are related to that found in the fermentation water. However, the methyl groups of both molecules are significantly different whatever the botanical origin of the carbon source in the fermentation medium.
Zhang
Ben-Li
BL
LAIEM, Université de Nantes, CNRS UMR6006, 2 rue de la Houssinière, B.P. 92208, 44322 03, Nantes Cedex, France. benli.zhang@chimbio.univ-nantes.fr
Fallourd
Valerie
V
Role
Catherine
C
Martin
Gérard J
GJ
D001711:Biotransformation; D002247:Carbon Isotopes; D003903:Deuterium; D000431:Ethanol / Q000737:chemistry / Q000378:metabolism*; D005285:Fermentation; D007553:Isotope Labeling; D019344:Lactic Acid / Q000737:chemistry / Q000378:metabolism*; D007778:Lactobacillus / Q000378:metabolism; D008333:Mandelic Acids / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013237:Stereoisomerism
D002247:Carbon Isotopes; D008333:Mandelic Acids; D019344:Lactic Acid; D000431:Ethanol; D003903:Deuterium; C037938:mandelic acid