Effects of metal ions on activity of plasmin.
The effects of some metal ions on amidolytic and fibrinogenolytic activities of highly purified human plasmin were investigated in vitro. In the presence of Zn2+, Cu2+, Cd2+, and Au+ in the incubation mixture at the concentrations of 1 x 10(-5) - 1x 10(-3) M, the amidolytic plasmin activity was strongly inhibited, whereas Ca2+ and Mg2+ at the same concentrations were not effective. The analysis of the kinetic study has shown that Zn2+ or Cu2+ acts as mixed-type inhibitors of plasmin activity. The inhibition of amidolytic plasmin activity by Zn2+ and Cu2+ was reduced in the presence of EDTA, histidine, or albumin. Incubation of plasmin with Zn2+ or Cu2+ (at the concentration of 5 x 10(-4) M) resulted in complete loss of its proteolytic action on fibrinogen, whereas Cd2+ and Au+ under the same conditions only partially inhibited this process.
Nowak
Pawel
P
Department of General Biochemistry, University of Lodz, Lodz, Poland.
Zgirski
Alojzy
A
D001798:Blood Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005341:Fibrinolysin / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006801:Humans; D007477:Ions / Q000494:pharmacology; D007700:Kinetics; D008670:Metals / Q000494:pharmacology*
D001798:Blood Proteins; D004791:Enzyme Inhibitors; D007477:Ions; D008670:Metals; D005341:Fibrinolysin