title: Cytotoxic necrotizing factor 1 enhances reactive oxygen species-dependent transcription and secretion of proinflammatory cytokines in human uroepithelial cells.
abstract: Uropathogenic Escherichia coli strains frequently produce a Rho-activating protein toxin named cytotoxic necrotizing factor type 1 (CNF1). We herein report that CNF1 promotes transcription and release of tumor necrosis factor alpha, gamma interferon, interleukin-6 (IL-6), and IL-8 proinflammatory cytokines and increases the production of reactive oxygen species (ROS) in uroepithelial T24 cells. The antioxidant N-acetyl-L-cysteine counteracts these phenomena, a fact which suggests a role for ROS-mediated signaling in CNF1-induced proinflammatory cytokine production.
authors.affiliation: Department of Ultrastructures, Istituto Superiore di Sanità, 00161 Rome, Italy.
authors.forename: Loredana
authors.initials: L
authors.lastname: Falzano
authors.forename: Maria Giovanna
authors.initials: MG
authors.lastname: Quaranta
authors.forename: Sara
authors.initials: S
authors.lastname: Travaglione
authors.forename: Perla
authors.initials: P
authors.lastname: Filippini
authors.forename: Alessia
authors.initials: A
authors.lastname: Fabbri
authors.forename: Marina
authors.initials: M
authors.lastname: Viora
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Donelli
authors.forename: Carla
authors.initials: C
authors.lastname: Fiorentini
mesh_terms: D001427:Bacterial Toxins / Q000633:toxicity*; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis*; D003603:Cytotoxins / Q000633:toxicity*; D029968:Escherichia coli Proteins; D006801:Humans; D017382:Reactive Oxygen Species; D014158:Transcription, Genetic / Q000187:drug effects*; D001743:Urinary Bladder / Q000187:drug effects* / Q000378:metabolism
chemical_list: D001427:Bacterial Toxins; D016207:Cytokines; D003603:Cytotoxins; D029968:Escherichia coli Proteins; D017382:Reactive Oxygen Species; C060296:cytotoxic necrotizing factor type 1