Adduct formation between oxanine and amine derivatives.
Oxanine (Oxa) is a major guanine lesion produced by nitric oxide (NO) under aerobic conditions. To elucidate the genotoxic mechanism of Oxa, this lesion was site-specifically incorporated into an oligonucleotide and allowed to react with cellular amines. Analysis of the reaction product revealed that Oxa formed adducts with spermidine and lysine, suggesting a novel genotoxic mechanism associated with NO-induced DNA damage.
Nakano
T
T
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima 739-8526, Japan.
Terato
H
H
Asagoshi
K
K
Ohyama
Y
Y
Suzuki
T
T
Yamada
M
M
Makino
K
K
Ide
H
H
D000588:Amines / Q000737:chemistry*; D018736:DNA Adducts / Q000138:chemical synthesis* / Q000737:chemistry; D008239:Lysine / Q000737:chemistry; D011684:Purine Nucleosides / Q000737:chemistry*; D013095:Spermidine / Q000737:chemistry
D000588:Amines; D018736:DNA Adducts; D011684:Purine Nucleosides; C468766:oxanine; D008239:Lysine; D013095:Spermidine