An activity associated with human chromosome 21 permits nuclear colocalization of the adenovirus E1B-55K and E4orf6 proteins and promotes viral late gene expression.
The adenovirus E1B-55K and E4orf6 proteins cooperate during virus infection while performing several tasks that contribute to a productive infection, including the selective nucleocytoplasmic transport of late viral mRNA. Previous studies have shown that the E4orf6 protein retains the E1B-55K protein in the nucleus of human and monkey cells, but not in those of rodents, suggesting that primate-specific cellular factors contribute to the E4orf6-mediated retention of the E1B-55K protein in the nucleus. In an effort to identify these proposed primate-specific cellular factors, the interaction of the E1B-55K and E4orf6 proteins was studied in a panel of stable human-rodent monochromosomal somatic cell hybrids. Analysis of this panel of cell lines has demonstrated the existence of an activity associated with human chromosome 21 that permits the E1B-55K and E4orf6 proteins to colocalize in the nucleus of a rodent cell. Additional hybrid cells bearing portions of human chromosome 21 were used to map this activity to a 10-megabase-pair segment of the chromosome, extending from 21q22.12 to a region near the q terminus. Strikingly, this region also facilitates the expression of adenovirus late genes in a rodent cell background while having little impact on the expression of early viral genes.
Chastain-Moore
Amy M
AM
Department of Microbiology and Immunology, Wake Forest University School of Medicine, Wake Forest University, Winston-Salem, NC 27157-1064, USA.
Roberts
Terry
T
Trott
Deborah A
DA
Newbold
Robert F
RF
Ornelles
David A
DA
D000256:Adenoviridae / Q000235:genetics*; D017501:Adenovirus E1B Proteins / Q000235:genetics / Q000378:metabolism*; D017504:Adenovirus E4 Proteins / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D002874:Chromosome Mapping; D002891:Chromosomes, Human, Pair 21 / Q000235:genetics* / Q000502:physiology; D015967:Gene Expression Regulation, Viral; D006367:HeLa Cells; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
D017501:Adenovirus E1B Proteins; D017504:Adenovirus E4 Proteins; D014764:Viral Proteins