Design and properties of trans-acting HDV ribozymes with extended substrate recognition regions.
We constructed a new type of trans-acting HDV ribozyme which is based on the antigenomic RNA sequence and has an additional binding site to form an extra stem (P5) of 7 base-pairs introduced in the J1/2 region between P1 and P2 stems. A substrate RNA containing the two binding sequences was specifically cleaved while no selectivity was observed in the case of the wild-type ribozyme with only one binding site. Mutation to produce two mismatch base-pairs in the central part of the P5 stem abolished the specific cleavage.
Hori
T
T
Department of Environment and Natural Sciences, Graduate School of Environment and Information Sciences, Yokohama National University, 79-7 Tokiwadai, Hodogaya-ku, Yokohama 240-8501, Japan.
Guo
F
F
Tanaka
Y
Y
Uesugi
S
S
D001483:Base Sequence; D001665:Binding Sites; D003698:Hepatitis Delta Virus / Q000201:enzymology*; D007700:Kinetics; D008969:Molecular Sequence Data; D009154:Mutation; D012313:RNA / Q000378:metabolism; D016337:RNA, Catalytic / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity
D016337:RNA, Catalytic; D012313:RNA