title: A targeting motif involved in sodium channel clustering at the axonal initial segment.
abstract: The sorting of sodium channels to axons and the formation of clusters are of primary importance for neuronal electrogenesis. Here, we showed that the cytoplasmic loop connecting domains II and III of the Nav1 subunit contains a determinant conferring compartmentalization in the axonal initial segment of rat hippocampal neurons. Expression of a soluble Nav1.2II-III linker protein led to the disorganization of endogenous sodium channels. The motif was sufficient to redirect a somatodendritic potassium channel to the axonal initial segment, a process involving association with ankyrin G. Thus, this motif may play a fundamental role in controlling electrical excitability during development and plasticity.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale Unité 464, Institut Jean Roche, Université de la Méditerranée, Faculté de Médecine Secteur-Nord, Boulevard P. Dramard, 13916 Marseille Cedex 20, France.
authors.forename: Juan José
authors.initials: JJ
authors.lastname: Garrido
authors.forename: Pierre
authors.initials: P
authors.lastname: Giraud
authors.forename: Edmond
authors.initials: E
authors.lastname: Carlier
authors.forename: Fanny
authors.initials: F
authors.lastname: Fernandes
authors.forename: Anissa
authors.initials: A
authors.lastname: Moussif
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Fache
authors.forename: Dominique
authors.initials: D
authors.lastname: Debanne
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Dargent
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D017487:Ankyrins / Q000378:metabolism; D001369:Axons / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D051668:Delayed Rectifier Potassium Channels; D006624:Hippocampus / Q000166:cytology; D006801:Humans; D015640:Ion Channel Gating; D008969:Molecular Sequence Data; D009154:Mutation; D062551:NAV1.2 Voltage-Gated Sodium Channel; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000378:metabolism; D024642:Potassium Channels, Voltage-Gated; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D015222:Sodium Channels / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D017487:Ankyrins; D051668:Delayed Rectifier Potassium Channels; D062551:NAV1.2 Voltage-Gated Sodium Channel; D009419:Nerve Tissue Proteins; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D011993:Recombinant Fusion Proteins; C568251:SCN2A protein, human; C568253:Scn2A protein, rat; D015222:Sodium Channels