title: No physical activity x CETP 1b.-629 interaction effects on lipid profile.
abstract: OBJECTIVE
  Being physically active may improve the lipid profile by increasing high-density lipoprotein (HDL) cholesterol (HDL-C) concentrations. Cholesteryl ester transfer protein (CETP) transfers cholesteryl esters from HDL to lipoproteins of lower density. The potential interactive effects of physical activity and the CETP A-C polymorphism on exon 1b.-629 (CETP 1b.-629) with lipid profile were investigated in a population-based cross-sectional survey.
  
  
  METHODS
  1720 men and women, ages 35-74 yr, were randomly selected from the general adult population of Geneva, Switzerland, throughout 1999-2000. A validated physical activity questionnaire measured total energy expenditure and the percentage thereof used in high-intensity activities (% high-intensity activity, e.g., brisk walking, sports). CETP 1b.-629 was assayed with PCR and allele-specific oligonucleotide hybridization. The study had 80% power to detect lipid profile differences from 0.10 to 0.15 mmol x L-1 ( approximately 4-6 mg x dL-1) between gender-specific (CETP genotype x % high-intensity activity) subgroups.
  
  
  RESULTS
  HDL-C (mmol x L-1) was higher in men and women with the AA genotype (respectively, 1.26 and 1.56) versus those with CC (1.14 and 1.46) (P < 0.0001, P < 0.002). Before stratification into CETP subgroups, a greater % high-intensity activity (upper tertile) was associated with higher HDL-C only in men (1.24 vs 1.19 in lower tertile, P < 0.0005). However, no statistically significant (CETP 1b.-629 x % high-intensity activity) interactions with lipid profile were detected in either gender.
  
  
  CONCLUSIONS
  The effects of the CETP 1b.-629 A-C polymorphism on blood lipid concentrations appear to be unchanged by the different observed physical activity levels in a mostly sedentary population.
authors.affiliation: Division of Clinical Epidemiology, Geneva University Medical Center, Geneva University Hospitals, Geneva, Switzerland.
authors.forename: Martine S
authors.initials: MS
authors.lastname: Bernstein
authors.forename: Michael C
authors.initials: MC
authors.lastname: Costanza
authors.forename: Richard W
authors.initials: RW
authors.lastname: James
authors.forename: Michael A
authors.initials: MA
authors.lastname: Morris
authors.forename: François
authors.initials: F
authors.lastname: Cambien
authors.forename: Ségoléne
authors.initials: S
authors.lastname: Raoux
authors.forename: Alfredo
authors.initials: A
authors.lastname: Morabia
mesh_terms: D000328:Adult; D000368:Aged; D002352:Carrier Proteins / Q000235:genetics* / Q000494:pharmacology*; D053480:Cholesterol Ester Transfer Proteins; D008076:Cholesterol, HDL / Q000097:blood* / Q000378:metabolism; D003430:Cross-Sectional Studies; D004734:Energy Metabolism; D015444:Exercise / Q000502:physiology*; D005091:Exons / Q000235:genetics; D005260:Female; D005838:Genotype; D006023:Glycoproteins; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012737:Sex Factors
chemical_list: C506642:CETP protein, human; D002352:Carrier Proteins; D053480:Cholesterol Ester Transfer Proteins; D008076:Cholesterol, HDL; D006023:Glycoproteins