title: Ethyl acetate soluble fraction of Cnidium officinale MAKINO inhibits neuronal cell death by reduction of excessive nitric oxide production in lipopolysaccharide-treated rat hippocampal slice cultures and microglia cells.
abstract: In the present work, we found that the ethyl acetate-soluble fraction of Cnidium officinale MAKINO (COEA) decreased nitric oxide (NO) production in the lipopolysaccharide (LPS)-stimulated BV-2 and primary microglia and suppressed expression of inducible nitric oxide synthase (iNOS) in BV-2 cells with the same pattern of NO production. In addition, we showed that excessive NO production played an important role in neuronal cell death in LPS-treated rat hippocampal slice cultures. Our data suggest that the COEA inhibits neuronal cell death by reduction of excessive NO production in LPS-treated rat hippocampal slice cultures. The ethyl acetate-soluble fraction of C. officinale reduced propidium iodide uptake and NO production in cultured media at the same time.
authors.affiliation: Department of Herbal Pharmacology, Graduate School of East-West Medical Science, Kyung Hee University, Seoul, Korea.
authors.forename: Jeong Min
authors.initials: JM
authors.lastname: Kim
authors.forename: Dongwook
authors.initials: D
authors.lastname: Son
authors.forename: Pyeongjae
authors.initials: P
authors.lastname: Lee
authors.forename: Kang Jin
authors.initials: KJ
authors.lastname: Lee
authors.forename: Hocheol
authors.initials: H
authors.lastname: Kim
authors.forename: Sun Yeou
authors.initials: SY
authors.lastname: Kim
mesh_terms: D000085:Acetates; D000818:Animals; D015153:Blotting, Western; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival; D031048:Cnidium / Q000737:chemistry*; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects*; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology*; D017628:Microglia / Q000166:cytology / Q000187:drug effects*; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D009569:Nitric Oxide / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II; D010936:Plant Extracts / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D051381:Rats
chemical_list: D000085:Acetates; D008070:Lipopolysaccharides; D010936:Plant Extracts; D009569:Nitric Oxide; C007650:ethyl acetate; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat