Complex landscape of protein structural dynamics unveiled by nanosecond Laue crystallography.
Although conformational changes are essential for the function of proteins, little is known about their structural dynamics at atomic level resolution. Myoglobin (Mb) is the paradigm to investigate conformational dynamics because it is a simple globular heme protein displaying a photosensitivity of the iron-ligand bond. Upon laser photodissociation of carboxymyoglobin Mb a nonequilibrium population of protein structures is generated that relaxes over a broad time range extending from picoseconds to milliseconds. This process is associated with migration of the ligand to cavities in the matrix and with a reduction in the geminate rebinding rate by several orders of magnitude. Here we report nanosecond time-resolved Laue diffraction data to 1.55-A resolution on a Mb mutant, which depicts the sequence of structural events associated with this extended relaxation. Motions of the distal E-helix, including the mutated residue Gln-64(E7), and of the CD-turn are found to lag significantly (100-300 ns) behind local rearrangements around the heme such as heme tilting, iron motion out of the heme plane, and swinging of the mutated residue Tyr-29(B10), all of which occur promptly (< or =3 ns). Over the same delayed time range, CO is observed to migrate from a cavity distal to the heme known to bind xenon (called Xe4) to another such cavity proximal to the heme (Xe1). We propose that the extended relaxation of the globin moiety reflects reequilibration among conformational substates known to play an essential role in controlling protein function.
Bourgeois
Dominique
D
Laboratoire de Cristallographie et de Cristallogénèse des Protéines, UMR 9015, Institut de Biologie Structurale/Commissariat à l'Energie Atomique/Université Joseph Fourier, and European Synchrotron Radiation Facility, Grenoble, France. bourgeoi@lccp.ibs.fr
Vallone
Beatrice
B
Schotte
Friedrich
F
Arcovito
Alessandro
A
Miele
Adriana E
AE
Sciara
Giuliano
G
Wulff
Michael
M
Anfinrud
Philip
P
Brunori
Maurizio
M
D019943:Amino Acid Substitution; D000818:Animals; D001665:Binding Sites; D055592:Biophysical Phenomena; D001703:Biophysics; D018360:Crystallography, X-Ray / Q000379:methods; D006418:Heme / Q000737:chemistry; D008958:Models, Molecular; D009211:Myoglobin / Q000737:chemistry / Q000235:genetics / Q000528:radiation effects; D010782:Photolysis; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000528:radiation effects; D055672:Static Electricity; D013816:Thermodynamics
D009211:Myoglobin; D011506:Proteins; D011994:Recombinant Proteins; C018013:carboxymyoglobin; D006418:Heme