Normal coupling of brain benzodiazepine and neurosteroid modulatory sites on the GABA-A receptor complex in human hepatic encephalopathy.
To assess the possible implication of the allosteric coupling of different modulatory sites at the GABA-A receptor complex in hepatic encephalopathy (HE), we investigated in autopsied frontal cortex of six cirrhotic patients and six appropriately-matched controls, the modulatory effects of the benzodiazepine site agonist flunitrazepam on the binding of [3H]muscimol and the effect of the neurosteroid site agonist allopregnanolone (5alpha-pregnan-3alpha-ol-20-one) on the binding of [3H]muscimol and [3H]flunitrazepam. There were no significant differences in either the magnitude E(max): 11.5+/-1.1% (controls) versus 10.2+/-2.2% (HE patients) or the efficacy EC(50): 20.2+/-5.5 nM (controls) versus 17.7+/-6.2 nM (HE patients) of flunitrazepam modulation of [3H]muscimol binding. Allopregnanolone also showed modulation of both sites to a comparable extent in brain tissue from cirrhotic patients and controls E(max): [3H]muscimol, 15.1+/-2.8% (controls) versus 13.8+/-1.9% (HE patients); [3H]flunitrazepam, 17.9+/-2.3% (controls) versus 19.1+/-2.3% (HE patients), EC(50): [3H]muscimol, 386.5+/-25.8 nM (controls) versus 373.8+/-13.1 nM (HE patients); [3H]flunitrazepam, 49.8+/-22.9 nM (controls) versus 55.5+/-14.0 nM (HE patients). These findings demonstrate unequivocally that the GABA-A sites and their benzodiazepine and neurosteroid modulatory sites manifest normal allosteric coupling in brain in human HE. Therefore, if increased "GABAergic tone" is implicated in the pathophysiology of HE, this must be the consequence of increased brain concentrations of endogenous benzodiazepine and/or neurosteroid ligands for components of the GABA-A receptor complex rather than alterations of the receptor proteins themselves.
Ahboucha
Samir
S
Neuroscience Research Unit, CHUM-Campus Saint-Luc, 1058 St.-Denis, Que., H2X 3J4, Montreal, Canada.
Desjardins
Paul
P
Chatauret
Nicolas
N
Pomier-Layrargues
Gilles
G
Butterworth
Roger F
RF
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001921:Brain / Q000503:physiopathology*; D005260:Female; D005442:Flumazenil / Q000494:pharmacology; D005445:Flunitrazepam / Q000493:pharmacokinetics / Q000494:pharmacology; D006501:Hepatic Encephalopathy / Q000503:physiopathology*; D006801:Humans; D002532:Intracranial Aneurysm / Q000503:physiopathology; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D009118:Muscimol / Q000493:pharmacokinetics / Q000494:pharmacology; D011963:Receptors, GABA-A / Q000187:drug effects / Q000502:physiology*; D012016:Reference Values; D012772:Shock, Septic / Q000503:physiopathology
D011963:Receptors, GABA-A; D009118:Muscimol; D005442:Flumazenil; D005445:Flunitrazepam