The alpha-L-ribo-isomers of RNA and LNA (locked nucleic acid).
The phosphoramidite approach has been used for the automated synthesis of alpha-L-LNA, alpha-L-RNA, and oligomers composed of mixtures of alpha-L-LNA, alpha-L-RNA and DNA monomers. Binding studies revealed very efficient recognition of single-stranded DNA and RNA target oligonucleotide strands. alpha-L-LNAs were shown to be significantly stabilized towards 3'-exonucleolytic degradation. Duplexes formed between RNA and alpha-L-LNA induced E. coli RNase H-mediated RNA cleavage, albeit very slow, at high enzyme concentration.
Sørensen
M D
MD
Department of Chemistry, University of Copenhagen, Universitetsparken 5, DK-2100 Copenhagen, Denmark.
Kvaernø
L
L
Bryld
T
T
Håkansson
A E
AE
Keinicke
L
L
Verbeure
B
B
Herdewijn
P
P
Wengel
J
J
D004247:DNA / Q000737:chemistry; D004722:Endoribonucleases / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D005092:Exonucleases / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D009696:Nucleic Acids / Q000737:chemistry* / Q000378:metabolism; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D013237:Stereoisomerism
D009696:Nucleic Acids; D012313:RNA; D004247:DNA; D004722:Endoribonucleases; D005092:Exonucleases