The antinociceptive and anti-inflammatory effect of ethylacetate extracts from Bang-Poong (Radix ledebouriellae) on the Freund's adjuvant-induced arthritis in rats.
In this study, we aimed to determine the antinociceptive and/or anti-inflammatory effect of Bang-Poong (BP, Radix Ledebouriellae) on Freund's adjuvant-induced arthritis in rats. Traditionally, BP has been used to treat several inflammatory diseases such as arthritis. Whole BP is extracted into two fractions that were ethylacetate and hexane-soluble fractions. Adult Sprague-Dawley rats (n=30, 130-150 g) were subcutaneously administered by the Freund's complete adjuvant (FCA) into the plantar surface of right hindpaw. Twelve days after the injection of FCA, the rats initially showed typical inflammatory edema and arthritis-related symptoms on the contralateral side (i.e. left hindpaw). Both antinociceptive (evaluation of mechanical, thermal pain threshold and analysis of spinal Fos expression) and anti- inflammatory (evaluation of paw edema, serum interleukin-6 level and x-ray analysis) effect of BP extracts were examined. The ethylacetate fraction of BP (BPE) significantly suppressed the FCA-induced paw edema as well as the serum level of interleukin-6 and it alleviated the radiological changes. Moreover, both mechanical and thermal hyperalgesia were attenuated by the treatment of BPE. In addition, spinal Fos expression that was increased by FCA- injection was suppressed in BPE group. Therefore, this study showed that BPE produced significant both antinociceptive and anti-inflammatory effects on FCA- induced arthritis in rats, while hexane fraction of BP did not show these effects. In conclusion, it is suggested that the ethylacetate fraction of BP is recommended to alleviate the arthritis-related symptoms in human according to the results of this study.
Kim
Hyun-Woo
HW
Department of Veterinary Physiology, College of Veterinary Medicine, Seoul National University, Suwon, South Korea.
Kwon
Young-Bae
YB
Ham
Tae-Won
TW
Roh
Dae-Hyun
DH
Yoon
Seo-Yeon
SY
Han
Ho-Jae
HJ
Kang
Sung-Keel
SK
Lee
Hye-Jung
HJ
Mar
Woung-Chon
WC
Yang
Il-Suk
IS
Beitz
Alvin J
AJ
Lee
Jang-Hern
JH
D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001169:Arthritis, Experimental / Q000000981:diagnostic imaging / Q000188:drug therapy*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004487:Edema / Q000662:veterinary; D006614:Hindlimb / Q000000981:diagnostic imaging; D006930:Hyperalgesia / Q000662:veterinary; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D010147:Pain Measurement / Q000662:veterinary; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D011859:Radiography; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000378:metabolism
D000700:Analgesics; D000893:Anti-Inflammatory Agents; D004365:Drugs, Chinese Herbal; D015850:Interleukin-6; D010936:Plant Extracts; D016760:Proto-Oncogene Proteins c-fos