title: Effects of benzo[a]pyrene, 2-bromopropane, phenol and 2,3,7,8-tetrachlorodibenzo-p-dioxin on proinflammatory cytokines gene expression by mice spleen cells.
abstract: The detrimental effects of environmental pollutants on the health of the individual are generally accepted, although the mechanisms of these effects remain to be incompletely understood. In the present study, we examined the effects of B[a]P, 2-BP, phenol and TCDD on proinflammatory cytokine gene expression in mice spleen cells which were stimulated with anti-CD3. 10(-9)M TCDD increased IFNgamma and TNFalpha gene expression, but suppressed IL-1 gene expression. 10(-6)M phenol inhibited IL-1, IL-6 and TNFalpha gene expression, and 10(-6)M of 2-BP downregulated TNFalpha gene expression. However, 10(-6)M of B[a]P did not influence on IL-1, IL-6, IFNgamma and TNFalpha gene expression. These findings suggest that TCDD may impair the immune functions of mice by enhancing proinflammatory cytokines production, whereas phenol and 2-BP may impair the functions by inhibiting the production of these cytokines.
authors.affiliation: College of Veterinary Medicine, Chungnam National University, Daejeon 305-764, Korea.
authors.forename: Kim
authors.initials: K
authors.lastname: Ho-Jun
authors.forename: Bit-Na
authors.initials: BN
authors.lastname: Kang
authors.forename: Sung-Whan
authors.initials: SW
authors.lastname: Cho
authors.forename: Hwa-Young
authors.initials: HY
authors.lastname: Son
authors.forename: Kyu-Shik
authors.initials: KS
authors.lastname: Jeong
authors.forename: Sang-Joon
authors.initials: SJ
authors.lastname: Park
authors.forename: Sung-Ho
authors.initials: SH
authors.lastname: Kim
authors.forename: Se-Ra
authors.initials: SR
authors.lastname: Kim
authors.forename: Tae-Hwan
authors.initials: TH
authors.lastname: Kim
authors.forename: Mi-Young
authors.initials: MY
authors.lastname: An
authors.forename: Si-Yun
authors.initials: SY
authors.lastname: Ryu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001564:Benzo(a)pyrene / Q000633:toxicity*; D017252:CD3 Complex / Q000276:immunology; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D004785:Environmental Pollutants / Q000633:toxicity*; D015870:Gene Expression / Q000187:drug effects; D006842:Hydrocarbons, Brominated / Q000633:toxicity*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D019800:Phenol / Q000633:toxicity*; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity*; D012313:RNA / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000187:drug effects* / Q000378:metabolism
chemical_list: D017252:CD3 Complex; D016207:Cytokines; D004785:Environmental Pollutants; D006842:Hydrocarbons, Brominated; D000072317:Polychlorinated Dibenzodioxins; D019800:Phenol; D001564:Benzo(a)pyrene; D012313:RNA; C103736:2-bromopropane