Adjustment function among antioxidant substances in acatalasemic mouse brain and its enhancement by low-dose X-ray irradiation.
The catalase activities in blood and organs of the acatalasemic (C3H/AnLCsbCsb) mouse of the C3H strain are lower than those of the normal (C3H/AnLCsaCsa) mouse. We conducted a study to examine changes in the activities of antioxidant enzymes, such as catalase, superoxide dismutase (SOD) and glutathione peroxidase (GPX), the total gluathione content, and the lipid peroxide level in the brain, which is more sensitive to oxidative stress than other organs, at 3, 6, or 24 hr following X-ray irradiation at doses of 0.25, 0.5, or 5.0 Gy to the acatalasemic and the normal mice. No significant change in the lipid peroxide level in the acatalasemic mouse brain was seen under non-irradiation conditions. However, the acatalasemic mouse brain was more damaged than the normal mouse brain by excessive oxygen stress, such as a high-dose (5.0 Gy) X-ray. On the other hand, we found that, unlike 5.0 Gy X-ray, a relatively low-dose (0.5 Gy) irradiation specifically increased the activities of both catalase and GPX in the acatalasemic mouse brain making the activities closer to those in the normal mouse brain. These findings may indicate that the free radical reaction induced by the lack of catalase is more properly neutralized by low dose irradiation.
Yamaoka
Kiyonori
K
Medical Radioscience, Okayama University Medical School, 2-5-1 Shikata, Okayama-shi, Okayama 700-8558, Japan.
Nomura
Takaharu
T
Wang
Da-Hong
DH
Mori
Shuji
S
Taguchi
Takehito
T
Ishikawa
Tetsuya
T
Hanamoto
Katsumi
K
Kira
Shohei
S
D020642:Acatalasia / Q000378:metabolism*; D000222:Adaptation, Physiological / Q000502:physiology; D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism; D001921:Brain / Q000201:enzymology / Q000378:metabolism* / Q000528:radiation effects*; D002374:Catalase; D004307:Dose-Response Relationship, Radiation; D004789:Enzyme Activation / Q000528:radiation effects; D005978:Glutathione / Q000032:analysis / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000032:analysis / Q000378:metabolism; D008054:Lipid Peroxides / Q000032:analysis / Q000378:metabolism; D051379:Mice; D012016:Reference Values; D013045:Species Specificity; D013482:Superoxide Dismutase / Q000032:analysis / Q000378:metabolism; D014916:Whole-Body Irradiation
D000975:Antioxidants; D008054:Lipid Peroxides; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione