Dentate granule cell neurogenesis after seizures induced by pentylenetrazol in rats.
Epileptic seizures originating from the limbic system have been shown to stimulate the proliferation rate of granule cell precursors in the adult brain, but it is not clear if other type(s) of seizures have the similar effects. This study examined the effects of pentylenetrazol (PTZ)-induced generalized clonic seizures on dentate granule cell neurogenesis in adult rats. Using systemic bromodeoxyuridine (BrdU) to label dividing cells, we studied the proliferation rate of neural precursor cells in the dentate gyrus at various time points after PTZ-induced seizures. The double-label immunofluorescence with confocal microscopy was used to determine the newborn cell phenotypes. Quantitative analysis of BrdU labeling revealed a significant increase in the proliferation rate of neural precursor cells in the dentate gyrus 3, 7, and 14 days after seizures. The number of BrdU-labeled cells in the dentate gyrus returned to baseline levels by 28 days after the initial seizures. Most of newborn cells migrated into the granule cell layer from the subgranular zone, displayed the neuronal phenotype, and developed morphological characteristics of differentiated dentate granule cells. These results indicated that neuron proliferation in the dentate gyrus was enhanced during a time window (3-14 days) after PTZ-induced seizures. Its underlying mechanism is discussed.
Jiang
Wen
W
Department of Neurology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. wen.jiang@usask.ca
Wan
Qi
Q
Zhang
Zhi-Jun
ZJ
Wang
Wei-Dong
WD
Huang
Yuan-Gui
YG
Rao
Zhi-Ren
ZR
Zhang
Xia
X
D000818:Animals; D034961:Antigens, Nuclear / Q000378:metabolism; D001578:Benzopyrans; D001973:Bromodeoxyuridine / Q000378:metabolism / Q000493:pharmacokinetics; D064026:Calbindins; D002452:Cell Count; D002455:Cell Division / Q000502:physiology; D003292:Convulsants; D018891:Dentate Gyrus / Q000378:metabolism / Q000473:pathology*; D004195:Disease Models, Animal; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D016900:Neurofilament Proteins / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000473:pathology*; D010433:Pentylenetetrazole; D011838:Radiation-Sensitizing Agents / Q000378:metabolism / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D064030:S100 Calcium Binding Protein G / Q000378:metabolism; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*
D034961:Antigens, Nuclear; D001578:Benzopyrans; D064026:Calbindins; D003292:Convulsants; D005904:Glial Fibrillary Acidic Protein; D008869:Microtubule-Associated Proteins; D016900:Neurofilament Proteins; D011838:Radiation-Sensitizing Agents; D064030:S100 Calcium Binding Protein G; C046829:timefurone; C051774:neurofilament protein H; D001973:Bromodeoxyuridine; D010433:Pentylenetetrazole