Ciz1, Cip1 interacting zinc finger protein 1 binds the consensus DNA sequence ARYSR(0-2)YYAC.
The matrin 3 family of nuclear proteins consists of members with potentially diverse activities. Matrin 3 and NP220 share RNA-binding domains, and NP220 has been shown to recognize and bind to the DNA sequence, CCCCC (G/C). We have isolated and characterized another member of the matrin 3 family, designated NP94, from a medulloblastoma. This protein, also named Ciz1, has previously been characterized for its ability to interact with p21(Cip1/Waf1) and contains 3 zinc finger domains and a matrin 3-homologous domain 3. Our immunofluorescence and Northern blot analysis data indicate that Ciz1 is localized in the nucleus and is expressed in a wide range of tissues, especially the pancreas and the brain; within the brain, the highest message levels are found in the cerebellum. A modified selected and amplified binding (SAAB) sequence method was used to identify DNA sequences recognized by Ciz1. From the analysis of the retrieved SAAB sequences and verification using electrophoretic mobility shift assays, we formulated a consensus DNA sequence, ARYSR(0-2)YYAC, recognized by Ciz1. The potential activities of Ciz1, including those involved in brain tumorigenesis, are discussed.
Warder
Daryl E
DE
Department of Surgery, Division of Neurosurgery, University of Texas Medical Branch, Galveston, TX, USA. dwarder23@hotmail.com
Keherly
Michael J
MJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence / Q000235:genetics; D001665:Binding Sites; D015152:Blotting, Northern; D001932:Brain Neoplasms / Q000378:metabolism; D019556:COS Cells; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D024202:Electrophoretic Mobility Shift Assay; D015723:Gene Library; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D041681:NIH 3T3 Cells; D034521:Nuclear Matrix-Associated Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism; D009928:Organ Specificity; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D016335:Zinc Fingers
C400423:Ciz1 protein, human; D017931:DNA Primers; D018076:DNA, Complementary; D034521:Nuclear Matrix-Associated Proteins; D009687:Nuclear Proteins; D009838:Oligodeoxyribonucleotides; D016601:RNA-Binding Proteins; C573692:matrin-3 protein, mouse; D004247:DNA