title: Enteral immunotherapy in the treatment of chronic enterocolitis in interleukin-10-deficient mice.
abstract: OBJECTIVE
  Transgenic IL-10-deficient mice (IL-10 ko mice) spontaneously develop a chronic inflammatory bowel disease that is reminiscent of Crohn's disease. In a randomized, prospective, comparative study, we evaluated the effect of local, endoluminal immunotherapy by duodenal injection of mouse recombinant IL-10 (rm IL-10), in IL-10 ko mice with chronic enterocolitis; the first of its kind.
  
  
  METHODS
  Sixteen IL-10 ko mice received a monoinjection of rm IL-10 into the duodenum while a control group of 16 IL-10 ko mice received an injection of physiological saline. Histology of the entire bowel and plasma concentrations of IL-4, IL-6, IL-10, tumor necrosis factor-alpha, and interferon-gamma were analyzed on the 3rd or 7th postoperative day.
  
  
  RESULTS
  The histological features of the specimens from IL-10 ko mice were, non-specific, segmentary and located mainly in the ileum. Lesions were of more significance in mice injected with physiological saline than in those receiving IL-10 in the proximal ileum, near to the site of injection. Plasma concentrations of IL-6 were higher in IL-10 ko mice than in the control group.
  
  
  CONCLUSIONS
  This study confirms that there is hyperproduction of Th1 cytokines in IL-10 ko mice and also suggests that endoluminal administration of IL-10 may be envisaged for the treatment or prevention of enterocolitis.
authors.affiliation: Surgical Department, Centre Hospitalo-Universitaire Lyon-Sud, Laboratoire Hyperthermie-Oncologie EA 643, Université Lyon 1, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Glehen
authors.forename: François
authors.initials: F
authors.lastname: Mithieux
authors.forename: Alexandra
authors.initials: A
authors.lastname: Traverse-Glehen
authors.forename: Sylvie
authors.initials: S
authors.lastname: Isaac
authors.forename: Jacques
authors.initials: J
authors.lastname: Bienvenu
authors.forename: Yves
authors.initials: Y
authors.lastname: François
authors.forename: Raymond
authors.initials: R
authors.lastname: Chung
authors.forename: François-Noël
authors.initials: FN
authors.lastname: Gilly
mesh_terms: D000818:Animals; D003424:Crohn Disease / Q000276:immunology* / Q000473:pathology; D004195:Disease Models, Animal; D004386:Duodenum; D004760:Enterocolitis / Q000276:immunology* / Q000473:pathology; D005260:Female; D007082:Ileum / Q000276:immunology / Q000473:pathology; D007167:Immunotherapy; D007267:Injections; D016753:Interleukin-10 / Q000008:administration & dosage* / Q000172:deficiency* / Q000235:genetics; D007413:Intestinal Mucosa / Q000276:immunology / Q000473:pathology; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D011994:Recombinant Proteins; D016753:Interleukin-10