title: Quantitative high-throughput analysis of drugs in biological matrices by mass spectrometry.
abstract: To support pharmacokinetic and drug metabolism studies, LC-MS/MS plays more and more an essential role for the quantitation of drugs and their metabolites in biological matrices. With the new challenges encountered in drug discovery and drug development, new strategies are put in place to achieve high-throughput analysis, using serial and parallel approaches. To speed-up method development and validation, generic approaches with the direct injection of biological fluids is highly desirable. Column-switching, using various packing materials for the extraction columns, is widely applied. Improvement of mass spectrometers performance, and in particular triple quadrupoles, also strongly influences sample preparation strategies, which remain a key element in the bioanalytical process.
authors.affiliation: University of Geneva, School of Pharmacy, Laboratory of Pharmaceutical Analytical Chemistry, Life Sciences Mass Spectrometry, 20 Bd d'Yvoy, CH-1211 Geneva 4, Switzerland.
authors.forename: Gérard
authors.initials: G
authors.lastname: Hopfgartner
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Bourgogne
mesh_terms: D000818:Animals; D001769:Blood; D004341:Drug Evaluation; D004353:Drug Evaluation, Preclinical; D006801:Humans; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000378:metabolism; D010599:Pharmacokinetics; D014556:Urine
chemical_list: D004364:Pharmaceutical Preparations