A novel potato defence-related alcohol:NADP+ oxidoreductase induced in response to Erwinia carotovora.
Identification of Solanum tuberosum genes responsive to culture filtrates (CF) from Erwinia carotovora subsp. carotovora led to the isolation of a full-length cDNA with high sequence similarity to several alcohol dehydrogenases. Accumulation of transcripts corresponding to this defence-related alcohol dehydrogenase (drd-1) was rapidly induced in CF-treated and wounded plants. The gene was also responsive to molecules involved in defence signalling such as salicylic acid, methyl jasmonate and ethylene. To elucidate the biochemical function of DRD-1, its cDNA was expressed in Escherichia coli. Enzymatic assays revealed that DRD-1 is an alcohol:NADP+ oxidoreductase with preference for various aromatic and aliphatic aldehydes. The enzyme exhibited high activity with several aldehydes including 2-methoxybenzaldehyde, 3-methoxybenzaldehyde, salicylaldehyde, o-vanillin, cinnamaldehyde, hydrocinnamaldehyde, hexanal and octanal. Identification of the reaction product by thin-layer chromatography confirmed the reduction of aldehydes to alcohols. Enzymatic activity measured with 2-methoxybenzaldehyde as a substrate was increased in salicylic acid- or methyl jasmonate-treated plants. These data suggest that DRD-1 may play an important role in potato defence response to Erwinia carotovora.
Montesano
Marcos
M
Department of Biosciences, Division of Genetics, University of Helsinki, Box 56, 00014 Finland.
Hyytiäinen
Heidi
H
Wettstein
Rodolfo
R
Palva
E Tapio
ET
D000085:Acetates / Q000494:pharmacology; D000429:Alcohol Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D002855:Chromatography, Thin Layer; D017077:Culture Media, Conditioned / Q000494:pharmacology; D003517:Cyclopentanes / Q000494:pharmacology; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004789:Enzyme Activation / Q000187:drug effects; D004885:Erwinia / Q000254:growth & development*; D005030:Ethylenes / Q000494:pharmacology; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D007700:Kinetics; D008969:Molecular Sequence Data; D054883:Oxylipins; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D020156:Salicylic Acid / Q000494:pharmacology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D011198:Solanum tuberosum / Q000201:enzymology / Q000235:genetics* / Q000382:microbiology; D013379:Substrate Specificity
D000085:Acetates; D017077:Culture Media, Conditioned; D003517:Cyclopentanes; D018076:DNA, Complementary; D005030:Ethylenes; D054883:Oxylipins; D012333:RNA, Messenger; C072239:methyl jasmonate; C036216:ethylene; D000429:Alcohol Oxidoreductases; C084155:alcohol dehydrogenase (NAD(P)+); D020156:Salicylic Acid