title: Chymase-positive mast cells in small sized adenocarcinoma of the lung.
abstract: Mast cells accumulate in angiogenesis-dependent situations of lung adenocarcinoma. Human mast cells are divided into two major subsets: MCT (mast cells with immunoreactivity for tryptase but not chymase) and MCTC (reactive for tryptase and chymase). Chymase is an important mediator of tissue remodeling, but research into chymase-containing mast cell subpopulations has been hampered by the lack of reagents suitable for use with formalin-fixed tissue. We stained chymase using CC1 antibody in 66 cases of small sized lung adenocarcinoma as well as CD34 and tryptase. There were significant positive correlations of microvessel counts with MCT-type and MCTC-type mast cell counts in lung adenocarcinomas. When analyzed according to Noguchi's classification, MCT-type and MCTC-type mast cells were significantly increased in Noguchi type-C tumors [localized bronchioloalveolar carcinoma (LBAC) with active fibroblastic proliferation] compared with in Noguchi type-A (LBAC) plus type-B tumors (LBAC with alveolar collapse). Members in the high-count group of MCTC-type but not MCT-type mast cells showed a significantly worse outcome than those in the low-count group in LBACs. Counting chymase-positive (MCTC-type) mast cells in tumor stroma may be a good prognosis predictor for LBACs, especially Noguchi type-C tumors.
authors.affiliation: Third Department of Internal Medicine, Sapporo Medical University School of Medicine, South-1, West-16 Chuo-ku, 060-8543 Sapporo, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Nagata
authors.forename: Noriharu
authors.initials: N
authors.lastname: Shijubo
authors.forename: Andrew F
authors.initials: AF
authors.lastname: Walls
authors.forename: Shingo
authors.initials: S
authors.lastname: Ichimiya
authors.forename: Shosaku
authors.initials: S
authors.lastname: Abe
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Sato
mesh_terms: D000230:Adenocarcinoma / Q000098:blood supply / Q000201:enzymology / Q000473:pathology*; D000804:Angiotensin II / Q000032:analysis; D002452:Cell Count; D053818:Chymases; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000098:blood supply / Q000201:enzymology / Q000473:pathology*; D008297:Male; D008407:Mast Cells / Q000201:enzymology*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D012697:Serine Endopeptidases / Q000032:analysis*; D053802:Tryptases
chemical_list: D000804:Angiotensin II; D012697:Serine Endopeptidases; D053818:Chymases; D053802:Tryptases; D020780:Matrix Metalloproteinase 9