Spatial profiling invertebrate ganglia using MALDI MS.
The ability of MALDI TOF MS to spatially map peptides and proteins directly from a tissue is an exciting advance to imaging mass spectrometry. Recent advances in instrumentation for MS have resulted in instruments capable of achieving several micron spatial resolution while acquiring high-resolution mass spectra. Currently, the ability to obtain high quality mass spectrometric images depends on sample preparation protocols that often result in limited spatial resolution. A number of sample preparation and matrix deposition protocols are evaluated for spatial profiling of Aplysia californica exocrine gland and neuronal tissues. Such samples are different from mammalian tissues, but make good targets for method optimization because of the wealth of biochemical information available on neuropeptide processing and distribution. Electrospray matrix deposition and a variety of freezing methods have been found to be optimum for these invertebrate tissues, with the exact protocols being tissue dependent.
Kruse
Rebecca
R
Department of Chemistry and the Beckman Institute, University of Illinois, Urbana, Illinois 61801, USA.
Sweedler
Jonathan V
JV
D000818:Animals; D001048:Aplysia / Q000033:anatomy & histology / Q000378:metabolism; D017952:Ganglia, Invertebrate / Q000737:chemistry* / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D009419:Nerve Tissue Proteins / Q000032:analysis*; D010455:Peptides / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D009419:Nerve Tissue Proteins; D010455:Peptides