A new method for construction of Ti plasmid-less strains in Agrobacterium tumefaciens.
Agrobacterium tumefaciens harboring a Ti plasmid causes plant tumorigenesis and it's DNA transfer system have been extensively used in plant biotechnology. Because Ti plasmids are high stable in agrobacterial hosts, their curing is very difficult by conventional methods. Here, we introduced a novel curing method by using Ti plasmids incompatibility.
Uraji
M
M
Department of Biological Science, Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8526, Japan.
Suzuki
K
K
Yoshida
K
K
D016960:Agrobacterium tumefaciens / Q000235:genetics*; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000235:genetics; D018014:Gene Transfer Techniques; D036741:Plant Tumor-Inducing Plasmids; D010957:Plasmids