Expression patterns of the transcription factor AP-2alpha during hair follicle morphogenesis and cycling.
AP-2alpha is a member of a family of transcription factors expressed in cells of the epithelial and neural crest lineage. AP-2alpha plays an essential role in embryonic development and in regulation of epithelial gene transcription. To further characterize the role of AP-2alpha in skin biology, we assessed its expression in the skin of C57BL/6J mice during defined stages of hair follicle morphogenesis and cycling. During early hair follicle morphogenesis, AP-2alpha was upregulated in the epidermal placode, in the basal keratinocytes of the hair follicle bud, and then in the inner root sheath. The follicular papilla cells underwent a brief upregulation of AP-2alpha expression during the initiation of hair shaft formation and active hair follicle downward growth. Completion of hair follicle morphogenesis was associated with a marked reduction of AP-2alpha immunoreactivity in the lower portion of the hair follicle including both epithelial and mesenchymal compartments. In adolescent mouse skin, consistently strong AP-2alpha expression was found in the basal keratinocytes of the epidermis, in the hair follicle infundibulum, and in the sebocytes. In the follicular papilla, AP-2alpha was weakly expressed in telogen, significantly upregulated in early anagen, then gradually declined, and reappeared again in middle catagen. In the inner root sheaths, AP-2alpha expression was detected during early and middle anagen and during middle catagen stages. Prominent AP-2alpha expression was also seen in the zone of club hair formation. Therefore, AP-2alpha upregulation in both epithelial and mesenchymal hair follicle compartments was coordinated with initiation of major remodeling processes. Our findings support the use of the hair follicle as a model to explore the role of AP-2alpha in physiologic remodeling of developing organs and in reciprocal ectodermal-mesenchymal interactions.
Panteleyev
Andrey A
AA
Department of Dermatology, Columbia University, College of Physicians and Surgeons, New York, NY 10032, USA. ap374@columbia.edu
Mitchell
Pamela J
PJ
Paus
Ralf
R
Christiano
Angela M
AM
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D002453:Cell Cycle / Q000502:physiology; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D005455:Fluorescent Antibody Technique; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D018859:Hair Follicle / Q000166:cytology / Q000254:growth & development* / Q000502:physiology*; D006204:Hair Removal; D051379:Mice; D008810:Mice, Inbred C57BL; D050656:Transcription Factor AP-2; D014157:Transcription Factors / Q000235:genetics*
D004268:DNA-Binding Proteins; C493668:Tfap2a protein, mouse; D050656:Transcription Factor AP-2; D014157:Transcription Factors