The effect of high and low ambient temperatures on PGE2 and TNF-alpha production by rat glial brain cultures.
This study was undertaken to investigate the effect of changes in incubation temperature on PGE(2) and TNF-alpha production by rat glial brain cultures after LPS stimulation. One-hour incubation at temperatures of 4 degrees C, 37 degrees C, 39 degrees C and 42 degrees C were used. Treatment of cultures with 10 microg/ml LPS from Escherichia coli caused a significant elevation of PGE(2) production 24 h after incubation at control temperatures of 37 degrees C and the experimental temperatures of 4 degrees C, 39 degrees C and 42 degrees C. While high ambient temperatures of 39 degrees C and 42 degrees C reduced LPS-stimulated production, compared to exposure to 37 degrees C, exposure to 4 degrees C did not do so. On the other hand, exposure of the cultures to a temperature of 39 degrees C and 42 degrees C for 1 h did not alter LPS-stimulated TNF-alpha production, while exposure to a temperature of 4 degrees C significantly reduced this elevation.
Shemi
D
D
Department of Clinical Pharmacology, Ben-Gurion University of the Negev, PO Box 653, Beer Sheva 84105, Israel.
Azab
Abed
A
Kaplanski
Jacob
J
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D003080:Cold Temperature; D015232:Dinoprostone / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000276:immunology; D006358:Hot Temperature; D008070:Lipopolysaccharides / Q000494:pharmacology; D009457:Neuroglia / Q000187:drug effects / Q000378:metabolism*; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D015232:Dinoprostone