Anomalous difference signal in protein crystals.
A scattering-angle-dependent formula is obtained for estimation of anomalous diffraction signals in Friedel pairs in protein crystals. In addition to the angle dependences of atomic form factor and temperature factor, a correlation form factor is introduced for the first time to take into account anomalous-scattering clusters such as disulfide bonds. Good agreements with experimental observations suggest that such correlated anomalous difference signals may be visible from measurements before a complete structural solution is obtained.
Shen
Qun
Q
Cornell High Energy Synchrotron Source (CHESS), Wilson Laboratory, Cornell University, Ithaca, NY 14853, USA. qs11@cornell.edu
Wang
Jun
J
Ealick
Steven E
SE
D003460:Crystallization; D008962:Models, Theoretical; D011506:Proteins / Q000737:chemistry*; D014961:X-Ray Diffraction / Q000379:methods*
D011506:Proteins