A modified mordant technique for staining plant chromosomes.
The ability to produce ferric acetate in house has provided a new mordant source for use in Newcomer's fluid (6:3:1:1:1 isopropanol, propionic acid, acetone, petroleum ether, 1,4-dioxane) for species with small chromosomes. This method improves on one first published in 1970.
Kubik
T J
TJ
Dow AgroSciences Canada Inc., 101-421 Downey Road, Saskatoon, SK S7N 4L8, Canada.
Hawkins
G P
GP
Stringam
G R
GR
D032461:Chromosomes, Plant / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods*
D005290:Ferric Compounds; C047434:ferric acetate