title: [Bacterial and fungal flora in some clinical materials in children with diabetes type 1].
abstract: BACKGROUND
  Children with poorly controlled diabetes are exposed to infections often caused by endogenous flora.
  
  
  OBJECTIVE
  To estimate incidence of pathogenic and opportunistic bacteria and fungi of Candida species.
  
  
  METHODS
  The urine, smears from the oral cavity and the pharynx as well as from the urogenital tract were examined in 130 children (62 boys and 68 girls) hospitalised because of newly diagnosed (53 children) or poorly controlled diabetes (77 patients). 29 children with short stature were the control group, in these children only the urine and smears from the oral cavity and the pharynx were only examined. Culture and identification of microorganisms were performed according to the methods used in microbiological diagnosis. Antifungal susceptibility testing was estimated with FUNGITEST Sanofi Pasteur and ATB Fungus bioMérieux.
  
  
  RESULTS
  Staphylococcus aureus in 43 diabetic children (33%) was observed. Staphylococcus aureus from different clinical materials was isolated; there was no difference in the frequency of occurrence of S. aureus in flora of the oral cavity and the pharynx in diabetic children in comparison with healthy children. Streptococcus group B in 29 (22.3%) was isolated, among them in 10 children from the pharynx. In healthy children Streptococcus group B was not observed. A significant bacteriuria was observed in 15 diabetic children (11.5%) and in 2 healthy children (6.9%). Fungi in 58 diabetic children (44.6%) were observed. Most often isolated species was Candida albicans (49 children), rarely other species. Candida spp. from different clinical materials was isolated, also in a non significant amount from the urine.
  
  
  CONCLUSIONS
  Microbial analysis of vagina should be routinely performed in diabetic adolescents. Urine of diabetics should be examined towards fungi.
authors.forename: Maria
authors.initials: M
authors.lastname: Nowakowska
authors.forename: Przemysława
authors.initials: P
authors.lastname: Jarosz-Chobot