title: The modified Q-cycle explains the apparent mismatch between the kinetics of reduction of cytochromes c1 and bH in the bc1 complex.
abstract: Crystallographic structures of the bc1 complex from different sources have provided evidence that a movement of the Rieske iron-sulfur protein (ISP) extrinsic domain is essential for catalysis. This dynamic feature has opened up the question of what limits electron transfer, and several authors have suggested that movement of the ISP head, or gating of such movement, is rate-limiting. Measurements of the kinetics of cytochromes and of the electrochromic shift of carotenoids, following flash activation through the reaction center in chromatophore membranes from Rhodobacter sphaeroides, have allowed us to demonstrate that: (i) ubiquinol oxidation at the Qo-site of the bc1 complex has the same rate in the absence or presence of antimycin bound at the Qi-site, and is the reaction limiting turnover. (ii) Activation energies for transient processes to which movement of the ISP must contribute are much lower than that of the rate-limiting step. (iii) Comparison of experimental data with a simple mathematical model demonstrates that the kinetics of reduction of cytochromes c1 and bH are fully explained by the modified Q-cycle. (iv) All rates for processes associated with movement of the ISP are more rapid by at least an order of magnitude than the rate of ubiquinol oxidation. (v) Movement of the ISP head does not introduce a significant delay in reduction of the high potential chain by quinol, and it is not necessary to invoke such a delay to explain the kinetic disparity between the kinetics of reduction of cytochromes c1 and bH.
authors.affiliation: Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA. a-crofts@life.uiuc.edu
authors.forename: Antony R
authors.initials: AR
authors.lastname: Crofts
authors.forename: Vladimir P
authors.initials: VP
authors.lastname: Shinkarev
authors.forename: Derrick R J
authors.initials: DR
authors.lastname: Kolling
authors.forename: Sangjin
authors.initials: S
authors.lastname: Hong
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology; D000968:Antimycin A / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D002384:Catalysis; D018360:Crystallography, X-Ray; D003574:Cytochrome c Group / Q000737:chemistry; D045303:Cytochromes b / Q000737:chemistry*; D003575:Cytochromes c1 / Q000737:chemistry*; D004579:Electron Transport; D014450:Electron Transport Complex III / Q000737:chemistry*; D007700:Kinetics; D008689:Methacrylates; D008954:Models, Biological; D008956:Models, Chemical; D008962:Models, Theoretical; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012242:Rhodobacter sphaeroides / Q000378:metabolism; D013053:Spectrophotometry; D013844:Thiazoles / Q000494:pharmacology; D013997:Time Factors
chemical_list: D000935:Antifungal Agents; D003574:Cytochrome c Group; D008689:Methacrylates; D013844:Thiazoles; C032456:antimycin; C046450:cytochrome C5; D000968:Antimycin A; C030517:myxothiazol; D045303:Cytochromes b; D003575:Cytochromes c1; D014450:Electron Transport Complex III; D010100:Oxygen