Effects of intracerebroventricular ethanol on ingestive behavior and induction of c-Fos immunoreactivity in selected brain regions.
The early changes in the central nervous system (CNS) following drinking of ethanol (ETOH) are poorly understood. It is known that chronic intracerebroventricular (ICV) administration of ethanol to rats induces preference for imbibed alcohol solutions. These results suggest that ICV ethanol could alter taste preference. In the present study, we tested whether ETOH[ICV] could induce a conditioned taste preference (CTP) or aversion (CTA) and alter c-Fos immunoreactivity (c-Fos-IR) in brain regions associated with feeding, aversion, and/or reward. Acute ETOH[ICV], as tested in the ETOH-naïve rat, did not induce CTA nor affect the amount of water imbibed by treated rats. The effects of ETOH[ICV] on intake and preference were determined using a novel palatable (i.e. sweet) noncaloric 0.1% saccharin solution. A single dose of ETOH[ICV] in the ETOH-nai;ve animal induced a CTP for saccharin. ETOH[ICV] significantly increased c-Fos-IR in a number of brain sites associated with feeding and reward including the bed nucleus of the stria terminalis, lateral dorsal area (BSTLD); nucleus accumbens, shell area (AcbSh); hypothalamic paraventricular nucleus (PVN); and lateral septum, ventral area (LSV). Thus, ETOH induced a CTP, not CTA, via central mechanisms; it increased c-Fos-IR in specific sites associated with feeding and reward.
Crankshaw
Daune L
DL
Department of Food Science and Nutrition, University of Minnesota, Twin Cities, St Paul, MN 55108, USA.
Briggs
Jacquie E
JE
Olszewski
Pawel K
PK
Shi
Qiuying
Q
Grace
Martha K
MK
Billington
Charles J
CJ
Levine
Allen S
AS
D000428:Alcohol Drinking / Q000235:genetics / Q000473:pathology / Q000503:physiopathology*; D000818:Animals; D001066:Appetite / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D001362:Avoidance Learning / Q000187:drug effects* / Q000502:physiology; D001921:Brain / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology; D003214:Conditioning, Classical / Q000187:drug effects* / Q000502:physiology; D000431:Ethanol / Q000494:pharmacology*; D005247:Feeding Behavior / Q000187:drug effects* / Q000502:physiology; D005518:Food Preferences / Q000187:drug effects / Q000502:physiology; D007276:Injections, Intraventricular; D008297:Male; D009042:Motivation; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013649:Taste / Q000187:drug effects* / Q000235:genetics / Q000502:physiology
D016760:Proto-Oncogene Proteins c-fos; D000431:Ethanol