Multilocus sequence typing of Staphylococcus aureus with DNA array technology.
A newly developed oligonucleotide array suited for multilocus sequence typing (MLST) of Staphylococcus aureus strains was analyzed with two strain collections in a two-center study. MLST allele identification for the first strain collection fully agreed with conventional strain typing. Analysis of strains from the second collection revealed that chip-defined MLST was concordant with conventional MLST. Array-mediated MLST data were reproducible, exchangeable, and epidemiologically concordant.
van Leeuwen
Willem B
WB
Department of Medical Microbiology & Infectious Diseases, Erasmus MC, 3015 GD Rotterdam, The Netherlands. vanleeuwen@erasmusmc.nl
Jay
Corinne
C
Snijders
Susan
S
Durin
Nathalia
N
Lacroix
Bruno
B
Verbrugh
Henry A
HA
Enright
Mark C
MC
Troesch
Alain
A
van Belkum
Alex
A
D000483:Alleles; D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D006801:Humans; D016106:Methicillin Resistance; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D017422:Sequence Analysis, DNA; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000145:classification* / Q000187:drug effects / Q000235:genetics*
D001426:Bacterial Proteins