Role of calcineurin in activity-dependent pattern formation in the dorsal lateral geniculate nucleus of the ferret.
In the retinogeniculate pathway of the ferret, in addition to the separation of the inputs from the two eyes to form eye-specific layers, there is also an anatomical segregation of the terminal arbors of on-center retinal ganglion cells from the terminal arbors of off-center retinal ganglion cell axons to form on/off sublaminae. Sublamination normally occurs during postnatal weeks 3-4 and requires the activity of retinal afferents, N-methyl-D-aspartate receptors, nitric oxide synthase, and a target of nitric oxide, cyclic guanosine monophosphate. Calcineurin is a calcium/calmodulin dependent serine, threonine protein phosphatase suggested to mediate NMDA-receptor dependent synaptic plasticity in the hippocampus. We have examined whether calcineurin plays a role during on/off sublamination in the dorsal lateral geniculate nucleus (dLGN) of the ferret. Immunohistochemistry showed that calcineurin expression is transiently up-regulated in dLGN cells and neuropil during the period of on/off sublamination. A functional role for calcineurin during sublamination was investigated by blocking the enzyme locally via intracranial infusion of FK506. Treatment with FK506 during postnatal weeks 3-4 disrupted the appearance of sublaminae. These results suggest that calcineurin may play a role during this process of activity-dependent pattern formation in the visual pathway.
Leamey
Catherine A
CA
Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Ho-Pao
Chrystal L
CL
Sur
Mriganka
M
D000344:Afferent Pathways / Q000502:physiology; D000818:Animals; D000831:Animals, Newborn; D000918:Antibody Specificity; D019703:Calcineurin / Q000096:biosynthesis / Q000502:physiology*; D005289:Ferrets / Q000502:physiology*; D005829:Geniculate Bodies / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D007150:Immunohistochemistry; D007166:Immunosuppressive Agents / Q000494:pharmacology; D009043:Motor Activity / Q000187:drug effects / Q000502:physiology*; D019581:Neuropil / Q000378:metabolism; D012160:Retina / Q000502:physiology; D016559:Tacrolimus / Q000494:pharmacology; D015854:Up-Regulation
D007166:Immunosuppressive Agents; D019703:Calcineurin; D016559:Tacrolimus