High-throughput FACS method for directed evolution of substrate specificity.
Olsen
Mark J
MJ
Department Chemistry and Biochemistry, University of Texas, Austin, TX, USA.
Gam
Jongsik
J
Iverson
Brent L
BL
Georgiou
George
G
D002384:Catalysis; D019020:Directed Molecular Evolution / Q000379:methods*; D004798:Enzymes / Q000235:genetics* / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D005434:Flow Cytometry / Q000379:methods; D005456:Fluorescent Dyes; D016296:Mutagenesis; D011994:Recombinant Proteins / Q000378:metabolism; D012680:Sensitivity and Specificity; D013379:Substrate Specificity
D004798:Enzymes; D005456:Fluorescent Dyes; D011994:Recombinant Proteins