Gas chromatographic method for determining very long chain fatty acids that compose D003 in aqueous suspensions from 20 to 200 mg/ml, used in pharmacological and toxicological studies.
D003 is a natural mixture of fatty acids (C(24:0) to C(36:0)), which shows antiplatelet, antithrombotic, and cholesterol-lowering effects in experimental models. A specific gas chromatographic method, using a BPX-5 wide-bore column and 1-nonadecanoic acid as internal standard, was developed and validated to determine the content of D003 in 20-200 mg/ml aqueous suspensions, which are used in pharmacological and toxicological studies. Fatty acids were extracted with chloroform and converted to methyl esters derivatives using 5% aqueous HCl-methanol. The method was linear for suspensions ranging from 10 to 250 mg/ml (correlation coefficient=0.9998) and showed a good accuracy, with average recoveries (98.5-101.28%) no significantly different from 100%, according to the Student t-test (P=0.05). The RSDs were <2.2%, indicating that the method has a good repeatability. The intermediate precision was good too, with RSDs between 1.20 and 2.10%. This method is suitable for quality control of these suspensions.
Díaz
Yanet Tejeda
YT
Center of Natural Products, National Center for Scientific Research, Cubanacán, Playa, PO Box 6414, Havana, Cuba.
Antolín
Ernesto Méndez
EM
Pérez
Roxana Sierra
RS
Delange
David Marrero
DM
Canavaciolo
Víctor González
VG
Granja
Abilio Laguna
AL
D002849:Chromatography, Gas / Q000379:methods*; D005227:Fatty Acids / Q000032:analysis* / Q000494:pharmacology* / Q000633:toxicity*; D018514:Plant Structures / Q000737:chemistry*; D011108:Polymers / Q000032:analysis*; D031786:Saccharum / Q000737:chemistry*; D013535:Suspensions / Q000737:chemistry
C478823:D003 preparation; D005227:Fatty Acids; D011108:Polymers; D013535:Suspensions