title: Characterization of DNA, RNA and DNA/RNA duplexes containing an L-nucleotide.
abstract: In order to investigate the duplex structure of DNA and RNA containing an L-nucleotide residue, we carried out the UV-melting and CD experiments. Although the introduction of an L-nucleotide into DNA/DNA, DNA/RNA and RNA/RNA duplexes reduced their Tm values, the typical two-state transitions were observed for all of the duplexes. The incorporation of an L-nucleotide into the RNA strand caused more remarkable decreases of the Tm value than that into the DNA strand. The CD spectra of the DNA/DNA and RNA/RNA duplexes containing an L-nucleotide are similar to those of unmodified duplexes. On the other hand, the spectra of the heterochiral DNA/RNA hybrid duplexes are significantly different from those of the homochiral DNA/RNA hybrid duplexes, which are generally thought to adopt the A-form.
authors.affiliation: Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Urata
authors.forename: H
authors.initials: H
authors.lastname: Shimizu
authors.forename: H
authors.initials: H
authors.lastname: Hiroaki
authors.forename: D
authors.initials: D
authors.lastname: Kohda
authors.forename: M
authors.initials: M
authors.lastname: Akagi
mesh_terms: D001483:Base Sequence; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D007536:Isomerism; D046911:Macromolecular Substances; D009690:Nucleic Acid Conformation; D009711:Nucleotides / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D013696:Temperature
chemical_list: D046911:Macromolecular Substances; D009711:Nucleotides; D012313:RNA; D004247:DNA