title: Specific regulation of lipocalin-type prostaglandin D synthase in mouse heart by estrogen receptor beta.
abstract: Estrogens have important physiological roles in the cardiovascular system. We use DNA microarray technology to study the molecular mechanism of estrogen action in the heart and to identify novel estrogen-regulated genes. In this investigation we identify genes that are regulated by chronic estrogen treatment of mouse heart. We present our detailed characterization of one of these genes, lipocalin-type prostaglandin D synthase (L-PGDS). Northern and Western blot analysis revealed that L-PGDS was induced both by acute and chronic estrogen treatment. Northern blot analysis, using estrogen receptor (ER)-disrupted mice, suggests that L-PGDS is specifically induced by ERbeta in vivo. In further support of ERbeta-selective regulation, we identify a functional estrogen-responsive element in the L-PGDS promoter, the activity of which is up-regulated by ERbeta, but not by ERalpha. We demonstrate that a one-nucleotide change (A to C) in the L-PGDS estrogen-responsive element affects receptor selectivity.
authors.affiliation: Department of Biosciences at Novum, Karolinska Institutet Huddinge SE-14157, Sweden. michio.otsuki@biosci.ki.se.
authors.forename: Michio
authors.initials: M
authors.lastname: Otsuki
authors.forename: Hui
authors.initials: H
authors.lastname: Gao
authors.forename: Karin
authors.initials: K
authors.lastname: Dahlman-Wright
authors.forename: Claes
authors.initials: C
authors.lastname: Ohlsson
authors.forename: Naomi
authors.initials: N
authors.lastname: Eguchi
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Urade
authors.forename: Jan-Ake
authors.initials: JA
authors.lastname: Gustafsson
mesh_terms: D000818:Animals; D004247:DNA / Q000378:metabolism; D047629:Estrogen Receptor beta; D004967:Estrogens / Q000494:pharmacology; D006321:Heart / Q000187:drug effects; D019746:Intramolecular Oxidoreductases / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D054834:Lipocalins; D051379:Mice; D009206:Myocardium / Q000201:enzymology* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011960:Receptors, Estrogen / Q000378:metabolism*
chemical_list: D047629:Estrogen Receptor beta; D004967:Estrogens; D054834:Lipocalins; D011960:Receptors, Estrogen; D004247:DNA; D019746:Intramolecular Oxidoreductases; C022466:prostaglandin R2 D-isomerase