title: Genetic stability assessment of plants regenerated from cryopreserved embryogenic tissues of Dioscorea bulbifera l. Using RAPD, biochemical and morphological analysis.
abstract: Embryogenic tissues of Dioscorea bulbifera were cryopreserved using the encapsulation-dehydration technique. Genetic stability of plants regenerated from cryopreserved embryogenic tissues was assessed using molecular, biochemical and morphological analysis. The random amplified polymorphic DNA (RAPD) analysis of 60 cryopreserved-derived and 20 in vitro grown (control) plantlets showed that 10 primers produced 62 clear reproducible DNA fragment profiles. The amplification products were monomorphic for all the plantlets except one. A total of 4960 DNA fragments were obtained from this study showing no variation in RAPD profiles. The diosgenin content of cryopreserved-derived plants, analyzed using HPLC, was similar to that of control plants. Morphology and the ability to form microtuber were also found to be unaltered in cryopreserved embryo-derived plantlets. Thus, the D. bulbifera plants regenerated from cryopreserved embryogenic tissues were genetically stable at the molecular, biochemical and morphological levels.
authors.affiliation: Tissue Culture and Cryopreservation Unit, National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi-110012, India.
authors.forename: Sonali
authors.initials: S
authors.lastname: Dixit
authors.forename: B B
authors.initials: BB
authors.lastname: Mandal
authors.forename: Sangeeta
authors.initials: S
authors.lastname: Ahuja
authors.forename: P S
authors.initials: PS
authors.lastname: Srivastava
mesh_terms: D002851:Chromatography, High Pressure Liquid; D015925:Cryopreservation / Q000379:methods*; D027742:Dioscorea / Q000196:embryology* / Q000235:genetics* / Q000502:physiology; D004144:Diosgenin / Q000032:analysis; D014644:Genetic Variation / Q000502:physiology*; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods; D012016:Reference Values; D012038:Regeneration / Q000235:genetics / Q000502:physiology
chemical_list: D004144:Diosgenin