A novel approach to selection of functional proteins.
We present here a novel approach to connect phenotype and genotype in vitro by exploiting a strong interaction between RNA and protein. This strong interaction was used for the selection of functional proteins, such as dihydrofolate reductase (DHFR) and streptavidin, which were chosen as examples. The noncovalent but strong interaction should be useful as a novel tool for the future selection of functional proteins.
Zhou
J M
JM
Gene Discovery Research Center, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-4 Higashi, Tsukuba Science City 305-8562, Japan.
Fujita
S
S
Warashina
M
M
Sawata
S Y
SY
Baba
T
T
Taira
K
K
D015723:Gene Library; D015696:Gene Products, tat / Q000378:metabolism; D005838:Genotype; D008957:Models, Genetic; D009843:Oligoribonucleotides / Q000378:metabolism; D010641:Phenotype; D011506:Proteins / Q000235:genetics* / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D019809:Streptavidin / Q000096:biosynthesis / Q000235:genetics; D013762:Tetrahydrofolate Dehydrogenase / Q000096:biosynthesis / Q000235:genetics
D015696:Gene Products, tat; D009843:Oligoribonucleotides; D011506:Proteins; D012333:RNA, Messenger; D019809:Streptavidin; D013762:Tetrahydrofolate Dehydrogenase