title: Single-molecule surface enhanced resonance Raman spectroscopy of the enhanced green fluorescent protein.
abstract: In the present contribution, we demonstrated that surface-enhanced resonance Raman scattering spectra from single green fluorescent proteins (GFPs) were obtained. The most important findings are the direct detection of the conversion between a deprotonated and a protonated form of the chromophore at the single-molecule level via the corresponding vibrational fingerprints, and the fact that the enhanced green fluorescent protein (EGFP) also shows a high surface enhanced resonance Raman scattering (SERRS) signal. Our findings show the potential of the technique to study structural dynamics of protein molecules at a single-molecule level.
authors.affiliation: Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200 F, 3001 Heverlee, Belgium.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Habuchi
authors.forename: Mircea
authors.initials: M
authors.lastname: Cotlet
authors.forename: Roel
authors.initials: R
authors.lastname: Gronheid
authors.forename: Gunter
authors.initials: G
authors.lastname: Dirix
authors.forename: Jan
authors.initials: J
authors.lastname: Michiels
authors.forename: Jos
authors.initials: J
authors.lastname: Vanderleyden
authors.forename: Frans C
authors.initials: FC
authors.lastname: De Schryver
authors.forename: Johan
authors.initials: J
authors.lastname: Hofkens
mesh_terms: D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins