A novel murine model of oral candidiasis with local symptoms characteristic of oral thrush.
A conventional and easy method to establish a murine oral candidiasis model, which has not only a stable yeast population in the oral cavity but also symptoms characteristic of oral thrush, was developed by using a sedative agent. Mice were immunosuppressed with prednisolone and were given tetracycline hydrochloride. They were orally infected with 10(6) viable cells of Candida albicans by means of a cotton swab and enough chlorpromazine chloride had been injected to keep them in a sedative state about for 3 hr after inoculation. From day 3 to day 7 post inoculation, 10(5)-10(6) colony forming units of Candida were recovered from the oral cavity of each mouse and whitish, curd-like patches were observed on most parts of tongue. Microscopically, germ tubes had appeared on the tongue surface. This model would be a useful experimental oral candidiasis for investigating the pathogenesis of C. albicans oral infection and the efficacy of various antifungal agents microbiologically and symptomatically.
Takakura
Natsuko
N
Nutritional Science Laboratory, Morinaga Milk Industry Co., Ltd., Zama, Kanagawa 228-8583, Japan. n-takakr@morinagamilk.co.jp
Sato
Yuichi
Y
Ishibashi
Hiroko
H
Oshima
Haruyuki
H
Uchida
Katsuhisa
K
Yamaguchi
Hideyo
H
Abe
Shigeru
S
D000666:Amphotericin B / Q000627:therapeutic use; D000818:Animals; D000935:Antifungal Agents / Q000627:therapeutic use; D002176:Candida albicans / Q000302:isolation & purification / Q000472:pathogenicity*; D002180:Candidiasis, Oral / Q000188:drug therapy / Q000382:microbiology / Q000503:physiopathology; D002746:Chlorpromazine / Q000008:administration & dosage; D004195:Disease Models, Animal; D005260:Female; D015725:Fluconazole / Q000627:therapeutic use; D016867:Immunocompromised Host; D051379:Mice; D008813:Mice, Inbred ICR; D011239:Prednisolone / Q000008:administration & dosage; D013752:Tetracycline / Q000008:administration & dosage; D014774:Virulence
D000935:Antifungal Agents; D000666:Amphotericin B; D015725:Fluconazole; D011239:Prednisolone; D013752:Tetracycline; D002746:Chlorpromazine