Site-specific recombination for genetic engineering in plants.
Site-specific recombination has been developed into a genetic engineering tool for higher eukaryotes. The manipulation of newly introduced DNA is now possible in the course of genetic transformation procedures, thus making the process more predictable and reliable. Also, a wide variety of chromosomal rearrangements using site-specific recombination have been documented both in metazoan and plant species. Applying such methods to plants opens new avenues for large-scale chromosome engineering in the future.
Lyznik
L A
LA
Transformation Research, Pioneer Hi-Bred International Inc., 7300 NW 62nd Avenue, Johnston, IA 50131, USA. alex.lyznik@pioneer.com
Gordon-Kamm
W J
WJ
Tao
Y
Y
D032461:Chromosomes, Plant / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D018014:Gene Transfer Techniques; D005818:Genetic Engineering / Q000379:methods*; D016297:Mutagenesis, Site-Directed / Q000235:genetics*; D059828:Plant Cells; D010944:Plants / Q000235:genetics*; D030821:Plants, Genetically Modified; D011995:Recombination, Genetic / Q000235:genetics*
D018744:DNA, Plant