title: A simple flow-through micro-chamber for handling fragile, small tissue explants and single non-adherent cells.
abstract: The flow-through chambers described are easily assembled in the laboratory with no need for sophisticated or expensive equipment. They allow for treating micro-explants or single non-adhesive cells with different solutes for defined times by easy and quick changes of the medium in the chambers. The specimen can be fixed, immunostained and observed microscopically within the chamber. During these procedures the cells are not exposed to undue mechanical stress and fragile intercellular structures are well preserved.
authors.affiliation: Max-Planck-Intitut für Entwicklungsbiologie, 72076 Tübingen Spemannstr. 35, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Hausen
authors.forename: Metta
authors.initials: M
authors.lastname: Riebesell
mesh_terms: D000818:Animals; D016477:Artifacts; D015815:Cell Adhesion Molecules / Q000187:drug effects / Q000378:metabolism; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods*; D002463:Cell Membrane Permeability / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured / Q000166:cytology* / Q000502:physiology; D003470:Culture Media / Q000494:pharmacology; D015194:Diffusion Chambers, Culture / Q000295:instrumentation* / Q000379:methods*; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000378:metabolism; D007150:Immunohistochemistry / Q000295:instrumentation / Q000379:methods; D008853:Microscopy / Q000295:instrumentation / Q000379:methods; D013314:Stress, Mechanical; D016707:Tissue Fixation / Q000295:instrumentation / Q000379:methods; D014357:Trypsin / Q000494:pharmacology; D014982:Xenopus laevis
chemical_list: D015815:Cell Adhesion Molecules; D003470:Culture Media; D014357:Trypsin