title: [Simple determination of residual anticoccidial drugs (diclazuril and nicarbazin) in chicken tissues by HPLC].
abstract: A simple and rapid determination of anticoccidial drug residues, diclazuril (DCZ) and nicarbazin (NCZ), in chicken tissues has been developed. DCZ and NCZ were extracted with acetonitrile from chicken liver, muscle, and fat. The extract was rinsed with n-hexane saturated with acetonitrile and then evaporated. The residue was dissolved in 1.4 mL of acetonitrile-methanol (1:1), then 1.0 mL of n-hexane saturated with acetonitrile-methanol (1:1) was added, and the mixture was partitioned by the addition of 0.6 mL of water. DCZ and NCZ in the aqueous layers were determined by HPLC on an Xterra RP-18 column with acetonitrile-0.5% ammonium acetate containing 0.01 mol/L tetra-n-butylammonium hydrogen sulfate (43:57) as the mobile phase. The mean recoveries (n = 5) of DCZ and NCZ spiked in chicken tissues at the maximum residue levels were 92.0-95.6% (CV 2.4-3.0%) and 87.3-89.4% (CV 1.7-2.8%), respectively. The detection limits of DCZ and NCZ were 0.01 and 0.004 microgram/g, respectively.
authors.affiliation: Tokyo Metropolitan Research Laboratory of Public Health: 3-24-1, Hyakunin-cho, Shinjuku-ku, Tokyo 169-0073, Japan.
authors.forename: Maki
authors.initials: M
authors.lastname: Kanda
authors.forename: Keiko
authors.initials: K
authors.lastname: Ushiyama
authors.forename: Kyoko
authors.initials: K
authors.lastname: Igusa
authors.forename: Mitsunori
authors.initials: M
authors.lastname: Murayama
authors.forename: Masakazu
authors.initials: M
authors.lastname: Horie
authors.forename: Masako
authors.initials: M
authors.lastname: Hirokado
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Miyazaki
mesh_terms: D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003049:Coccidiostats / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D009528:Nicarbazin / Q000032:analysis*; D009570:Nitriles / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*; D014227:Triazines / Q000032:analysis*
chemical_list: D003049:Coccidiostats; D009570:Nitriles; D010573:Pesticide Residues; D014227:Triazines; D009528:Nicarbazin; C057884:diclazuril