title: Noninvasive bone replacement with a new injectable calcium phosphate biomaterial.
abstract: The use of injectable calcium phosphate (CaP) biomaterials in noninvasive surgery should provide efficient bone colonization and implantation. Two different kinds of injectable biomaterials are presently under development: ionic hydraulic bone cements that harden in vivo after injection, and an association of biphasic calcium phosphate (BCP) ceramic granules and a water-soluble polymer vehicle (a technique particularly investigated by our group), providing an injectable CaP bone substitute (IBS). In our study, we compared these two approaches, using physicochemical characterizations and in vivo evaluations in light microscopy, scanning electron microscopy, and three-dimensional microtomography with synchrotron technology. Three weeks after implantation in rabbit bone, both biomaterials showed perfect biocompatibility and bioactivity, but new bone formation and degradation of the biomaterial were significantly greater for BCP granules than for ionic cement. Newly formed bone developed, binding the BCP granules together, whereas new bone grew only on the surface of the cement, which remained dense, with no obvious degradation 3 weeks after implantation. This study confirms that BCP granules carried by a cellulosic polymer conserve bioactivity and are conducive to earlier and more extensive bone substitution than a carbonated-hydroxyapatite bone cement. The presence of intergranular spaces in the BCP preparation, as shown on microtomography imaging, seems particularly favorable, allowing body fluids to reach each BCP granule immediately after implantation. Thus, the IBS functions as a completely interconnected ceramic with total open macroporosity. This new bone replacement approach should facilitate microinvasive bone surgery and local delivery of bone therapy agents.
authors.affiliation: Laboratoire de Recherche sur les Matériaux d'Intérêt Biologique INSERM 99-03, Faculté de Chirurgie Dentaire, 1 Place Alexis Ricordeau, 44042 Nantes Cedex 01, France.
authors.forename: O
authors.initials: O
authors.lastname: Gauthier
authors.forename: I
authors.initials: I
authors.lastname: Khairoun
authors.forename: J
authors.initials: J
authors.lastname: Bosco
authors.forename: L
authors.initials: L
authors.lastname: Obadia
authors.forename: X
authors.initials: X
authors.lastname: Bourges
authors.forename: C
authors.initials: C
authors.lastname: Rau
authors.forename: D
authors.initials: D
authors.lastname: Magne
authors.forename: J M
authors.initials: JM
authors.lastname: Bouler
authors.forename: E
authors.initials: E
authors.lastname: Aguado
authors.forename: G
authors.initials: G
authors.lastname: Daculsi
authors.forename: P
authors.initials: P
authors.lastname: Weiss
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001843:Bone Cements; D018786:Bone Substitutes; D002130:Calcium Phosphates / Q000008:administration & dosage* / Q000737:chemistry; D002516:Ceramics; D005269:Femur; D021621:Imaging, Three-Dimensional; D007267:Injections; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D009339:Needles; D016348:Osseointegration; D016062:Porosity; D011817:Rabbits; D013499:Surface Properties; D017356:Synchrotrons; D014054:Tomography / Q000379:methods; D014783:Viscosity
chemical_list: D001672:Biocompatible Materials; D001843:Bone Cements; D018786:Bone Substitutes; D002130:Calcium Phosphates; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; C485838:calcium phosphate, monobasic, anhydrous; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous