Differential gene expression in CD8+ cells exhibiting noncytotoxic anti-HIV activity.
Suppressive subtractive hybridization with polymerase chain reaction was used to identify the gene(s) associated with the CD8+ cell noncytotoxic anti-HIV response. The differences in gene expression profiles of CD8+ cells from a pair of discordant HIV-positive identical twins were studied. Forty-nine genes were identified as expressed at higher levels in the CD8+ cells from the infected twin that inhibited viral replication. The differential expression of these genes was then evaluated using Q-PCR to determine if this gene expression pattern is evident in CD8+ cells from other HIV-positive subjects showing this antiviral activity. Three genes, including one unknown, were found to have significantly increased expression in antiviral CD8+ cells.
Diaz
Leyla S
LS
Department of Medicine, Division of Hematology/Oncology, University of California, San Francisco, CA 94143-1270, USA.
Stone
Mars R
MR
Mackewicz
Carl E
CE
Levy
Jay A
JA
D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D003602:Cytotoxicity, Immunologic; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015723:Gene Library; D006678:HIV / Q000276:immunology*; D006801:Humans; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification
D012333:RNA, Messenger