title: Efficient transformation of Medicago truncatula cv. Jemalong using the hypervirulent Agrobacterium tumefaciens strain AGL1.
abstract: The efficiency of Agrobacterium tumefaciens transformation of the model legume Medicago truncatula cv. Jemalong (genotype 2HA) was evaluated for strains LBA 4404, C58pMP90, C58pGV2260 and AGL1. Binary vectors carrying promoter- gus/ gfp reporter gene fusions and the nptII gene as selectable marker were used for plant in vitro transformation/regeneration. The highest transformation efficiency was obtained with the disarmed hypervirulent strain AGL1 (Ti plasmid TiBo542), for which the percentage of explants forming kanamycin (Km)-resistant calli was double that obtained with each of the other three strains. In addition, we were able to reduce the time necessary for plant regeneration using AGL1, with 24% of the explants generating Km-resistant transgenic plantlets within only 4-5 months of culture. Transgene expression in planta was analysed and found to be conserved in the T(1) descendents.
authors.affiliation: Laboratoire des Interactions Plantes-Microorganismes, INRA-CNRS, UMR215, BP 27, 31326 Castanet Tolosan Cedex, France. mchabaud@toulouse.inra.fr
authors.forename: M
authors.initials: M
authors.lastname: Chabaud
authors.forename: F
authors.initials: F
authors.lastname: de Carvalho-Niebel
authors.forename: D G
authors.initials: DG
authors.lastname: Barker
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000254:growth & development*; D018506:Gene Expression Regulation, Plant; D005816:Genetic Complementation Test; D029902:Medicago / Q000235:genetics / Q000254:growth & development*; D030821:Plants, Genetically Modified; D014162:Transfection / Q000379:methods; D014170:Transformation, Genetic / Q000235:genetics*; D019076:Transgenes / Q000235:genetics