title: Biochemical and molecular characterization of a rice glutelin allele for the GluA-1 gene.
abstract: The rice ( Oryza sativa L.) mutant of glu4a, lacking the glutelin alpha-2 subunit while the alpha-1 subunit increased (alpha-1H/alpha-2L), was used in this study. Two-dimensional electrophoresis analysis revealed that the mutant lacked the polypeptide pI6.71/alpha-2 encoded by glu4 while forming a new polypeptide of pI6.50/alpha-1. Experiments were conducted to identify the relationships between the mutated polypeptides of the mutant and to illustrate the mutation mechanism of the allele. Peptide mapping and amino-acid sequence analyses revealed that the newly formed glu4a encoded polypeptide pI6.50/alpha-1 of high homology with the deleted pI6.71/alpha-2 polypeptide which was encoded by glu4 (GluA-1). The nucleotide sequence revealed that the iso-electric point variation of the pI6.50/alpha-1 polypeptide was caused by a point mutation with nucleotide replacement at the variable region of the gene. These results suggested the possibility of altering glutelin quality by using single gene mutation.
authors.affiliation: Institute of Genetics and Developmental Biology, the Chinese Academy of Sciences, Beijing 100101, China. lqu@affrc.go.jp
authors.forename: L Q
authors.initials: LQ
authors.lastname: Qu
authors.forename: X L
authors.initials: XL
authors.lastname: Wei
authors.forename: H
authors.initials: H
authors.lastname: Satoh
authors.forename: T
authors.initials: T
authors.lastname: Kumamaru
authors.forename: M
authors.initials: M
authors.lastname: Ogawa
authors.forename: F
authors.initials: F
authors.lastname: Takaiwa
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D018744:DNA, Plant / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D005983:Glutens / Q000235:genetics* / Q000378:metabolism; D007526:Isoelectric Point; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D010449:Peptide Mapping; D017354:Point Mutation; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
chemical_list: D018744:DNA, Plant; D005983:Glutens