Synthesis of a functionalized high affinity mannose receptor ligand and its application in the construction of peptide-, polyamide- and PNA-conjugates.
The synthesis of a high affinity mannose receptor ligand, appropriately functionalized for chemoselective ligation with an antigen or DNA-binding moieties is described. By a combination of solid- and solution-phase chemistry a versatile synthesis of the target structure was accomplished. Examples of subsequent ligation reactions are described.
Kinzel
Olaf
O
Department of Molecular and Cell Biology, IRBM P. Angeletti, Via Pontina Km 30.600, 00040 Pomezia (Rome), Italy.
Fattori
Daniela
D
Ingallinella
Paolo
P
Bianchi
Elisabetta
E
Pessi
Antonello
A
D000595:Amino Acid Sequence; D001667:Binding, Competitive; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000378:metabolism; D037181:Lectins, C-Type / Q000378:metabolism*; D008024:Ligands; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins / Q000378:metabolism*; D008956:Models, Chemical; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009757:Nylons / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism*; D013997:Time Factors
D037181:Lectins, C-Type; D008024:Ligands; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D009757:Nylons; D009842:Oligopeptides; D020135:Peptide Nucleic Acids; D011956:Receptors, Cell Surface; D004247:DNA