title: Crystallization and preliminary X-ray analysis of the human vascular adhesion protein-1.
abstract: Human vascular adhesion protein-1 (VAP-1) is a membrane-bound multifunctional glycoprotein with both adhesive and enzymatic properties. The protein belongs to the copper-containing amine oxidase (CAO) family, which use 2,4,5-trihydroxyphenylalanine quinone as a cofactor. Here, the crystallization and preliminary X-ray analysis of a mammalian CAO, human VAP-1, is reported. The protein was expressed in Chinese hamster ovary cells as a full-length form with an N-terminal transmembrane region and multiple glycosylation sites. Hexagonal crystals with unit-cell parameters a = b = 225.9, c = 218.7 A, alpha = beta = 90, gamma = 120 degrees were obtained using the vapour-diffusion method. Data from three different crystals were collected at 100 K using synchrotron radiation and were processed to 3.2 A resolution with 95.9% completeness and an R(merge) of 19.6%.
authors.affiliation: Department of Biochemistry and Pharmacy, Abo Akademi University, PO Box 66, FIN-20521 Turku, Finland.
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Nymalm
authors.forename: Heidi
authors.initials: H
authors.lastname: Kidron
authors.forename: Annu
authors.initials: A
authors.lastname: Söderholm
authors.forename: Lenita
authors.initials: L
authors.lastname: Viitanen
authors.forename: Kimmo
authors.initials: K
authors.lastname: Kaukonen
authors.forename: Marjo
authors.initials: M
authors.lastname: Pihlavisto
authors.forename: David
authors.initials: D
authors.lastname: Smith
authors.forename: Timo
authors.initials: T
authors.lastname: Veromaa
authors.forename: Tomi T
authors.initials: TT
authors.lastname: Airenne
authors.forename: Mark S
authors.initials: MS
authors.lastname: Johnson
authors.forename: Tiina A
authors.initials: TA
authors.lastname: Salminen
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000737:chemistry* / Q000302:isolation & purification; D015815:Cell Adhesion Molecules / Q000737:chemistry* / Q000302:isolation & purification; D003001:Cloning, Molecular; D003460:Crystallization; D006031:Glycosylation; D006801:Humans; D011994:Recombinant Proteins; D014961:X-Ray Diffraction
chemical_list: D015815:Cell Adhesion Molecules; D011994:Recombinant Proteins; C076668:AOC3 protein, human; D006631:Amine Oxidase (Copper-Containing)