A panoramic view of yeast noncoding RNA processing.
Predictive analysis using publicly available yeast functional genomics and proteomics data suggests that many more proteins may be involved in biogenesis of ribonucleoproteins than are currently known. Using a microarray that monitors abundance and processing of noncoding RNAs, we analyzed 468 yeast strains carrying mutations in protein-coding genes, most of which have not previously been associated with RNA or RNP synthesis. Many strains mutated in uncharacterized genes displayed aberrant noncoding RNA profiles. Ten factors involved in noncoding RNA biogenesis were verified by further experimentation, including a protein required for 20S pre-rRNA processing (Tsr2p), a protein associated with the nuclear exosome (Lrp1p), and a factor required for box C/D snoRNA accumulation (Bcd1p). These data present a global view of yeast noncoding RNA processing and confirm that many currently uncharacterized yeast proteins are involved in biogenesis of noncoding RNA.
Peng
Wen Tao
WT
Banting and Best Department of Medical Research, University of Toronto, 112 College Street, M5G 1L6, Toronto, Ontario, Canada.
Robinson
Mark D
MD
Mnaimneh
Sanie
S
Krogan
Nevan J
NJ
Cagney
Gerard
G
Morris
Quaid
Q
Davierwala
Armaity P
AP
Grigull
Jörg
J
Yang
Xueqi
X
Zhang
Wen
W
Mitsakakis
Nicholas
N
Ryan
Owen W
OW
Datta
Nira
N
Jojic
Vladimir
V
Pal
Chris
C
Canadien
Veronica
V
Richards
Dawn
D
Beattie
Bryan
B
Wu
Lani F
LF
Altschuler
Steven J
SJ
Roweis
Sam
S
Frey
Brendan J
BJ
Emili
Andrew
A
Greenblatt
Jack F
JF
Hughes
Timothy R
TR
D002478:Cells, Cultured; D005656:Fungal Proteins / Q000235:genetics / Q000302:isolation & purification; D015966:Gene Expression Regulation, Fungal / Q000235:genetics*; D016681:Genome, Fungal; D009154:Mutation / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D012322:RNA Precursors / Q000096:biosynthesis / Q000235:genetics; D020537:RNA, Small Nucleolar / Q000096:biosynthesis / Q000235:genetics; D012343:RNA, Transfer / Q000096:biosynthesis / Q000235:genetics; D022661:RNA, Untranslated / Q000096:biosynthesis* / Q000235:genetics; D012261:Ribonucleoproteins / Q000096:biosynthesis*; D015003:Yeasts / Q000235:genetics / Q000378:metabolism*
D005656:Fungal Proteins; D012322:RNA Precursors; D020537:RNA, Small Nucleolar; D022661:RNA, Untranslated; D012261:Ribonucleoproteins; D012343:RNA, Transfer