New base analogs for the formation of non-natural triplexes.
Novel nucleoside analogs have been designed for selective formation of antiparallel triplexes including a TA or a CG interrupting site. The new compounds are constructed of a W-shape bicyclic nucleic acid (WNA) bearing an aromatic ring as a stacking motif and a guanine for the formation of Hoogesteen hydrogen bonds, and are expected to effect triplex stabilization by both stacking and complementary hydrogen bonds. Purine-rich triplex-forming oligodeoxynucleotide (TFO) incorporating the new analog, WNA-7 beta G, formed a stable triplex with high selectivity to the AT site.
Sasaki
S
S
Graduate School of Pharmaceutical Sciences, Kyushu University, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Yamauchi
H
H
Takahasi
R
R
Taniguchi
Y
Y
Maeda
M
M
D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry; D005663:Furans / Q000138:chemical synthesis* / Q000737:chemistry; D006147:Guanine / Q000031:analogs & derivatives / Q000138:chemical synthesis* / Q000737:chemistry; D006860:Hydrogen Bonding; D008956:Models, Chemical; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry
D005663:Furans; D009838:Oligodeoxyribonucleotides; C476350:WNA 7betaG; C476351:WNA 9betaG; C051802:triplex DNA; D006147:Guanine; D004247:DNA