Evaluation of a new fiber-optic pressure recording system for cardiovascular measurements in mice.
We have tested a new fiber-optic pressure recording system, Samba, with a thin fiber [outer diameter (OD) = 0.25 mm] and a pressure sensor (length and OD = 0.42 mm) attached to the end. The accuracy of the system tested in vitro was good, with a coefficient of variation of 2.54% at 100 mmHg. The drift was <0.45 mmHg/h, and the temperature sensitivity was approximately 0.07 mmHg/1 degrees C between 22 and 37 degrees C. The frequency response characteristics were similar to a 1.4-Fr Millar catheter (0-200 Hz). Introduction of the Samba sensor from the right carotid artery into the left ventricle in six mice caused no drop in mean aortic pressure, whereas introduction of a 1.4-Fr Millar catheter (OD = 0.47 mm; n = 6) caused a pressure drop from 91.6 +/- 9.2 to 65.1 +/- 6.2 mmHg; P < 0.05. Thus the Samba sensor system may represent a new alternative to assess hemodynamic variables in the murine cardiovascular system.
Woldbaek
Per Reidar
PR
Institute for Experimental Medical Research, Ullevål University Hospital, University of Oslo, 0407 Oslo, Norway.
Stromme
Taevje Andreas
TA
Sande
Jørn Bodvar
JB
Christensen
Geir
G
Tønnessen
Theis
T
Ilebekk
Arnfinn
A
D000818:Animals; D001011:Aorta / Q000502:physiology*; D001794:Blood Pressure / Q000502:physiology*; D001795:Blood Pressure Determination / Q000295:instrumentation* / Q000592:standards; D005336:Fiber Optic Technology / Q000295:instrumentation* / Q000592:standards; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D055100:Optical Fibers; D015203:Reproducibility of Results; D013696:Temperature