title: Rapid appearance of pathological changes of neurons and glia cells in the cerebellum of microsphere-embolized rats.
abstract: Neuropathological changes in the cerebellar cortex of microsphere-embolized rats were studied at 30 min and 3 h after the embolism. Necrotic processes including a sponge-like vacuolation in the molecular layer, a vague outline of some Purkinje cells, and a few pyknotic granule cells having small and dark profiles were identified at sometime between 30 min and 3 h after microsphere-induced embolism in Nissl staining. Glial fibrillary acidic protein staining shows an apparent reduction in the number of Bergmann glial processes in some of the areas where there was necrosis of the molecular layer and poor astroglia processes in the areas subjacent to the pyknotic granule cells. These data demonstrate that within a short time, microsphere-induced cerebral ischemia produces necrosis of cerebellar neurons (i.e. Purkinje and granule cells) and changes in cerebellar glia cells (i.e. Bergmann and astroglia cells), and that these neuropathological changes are secondary phenomenon caused by microsphere blockage of cerebellar blood flow.
authors.affiliation: Department of Morphology, Tokai University School of Medicine, Bohseidai, Kanagawa 259-1193, Japan. smasaki@is.icc.u-tokai.c.jp
authors.forename: Masaki
authors.initials: M
authors.lastname: Sekiguchi
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Sugiyama
authors.forename: Keiko
authors.initials: K
authors.lastname: Takagi
authors.forename: Norio
authors.initials: N
authors.lastname: Takagi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Takeo
authors.forename: Osamu
authors.initials: O
authors.lastname: Tanaka
authors.forename: Ippei
authors.initials: I
authors.lastname: Yamato
authors.forename: Kojun
authors.initials: K
authors.lastname: Torigoe
authors.forename: R S
authors.initials: RS
authors.lastname: Nowakowski
mesh_terms: D000818:Animals; D002531:Cerebellum / Q000378:metabolism / Q000473:pathology*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D020766:Intracranial Embolism / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008863:Microspheres; D009457:Neuroglia / Q000378:metabolism / Q000473:pathology*; D009474:Neurons / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D005904:Glial Fibrillary Acidic Protein