Quantitation of DNA damage by an aldehyde reactive probe (ARP).
DNA is constantly damaged by endogenous and environmental agents. Abasic sites representing a major class of DNA damage can be quantitated by an ELISA-like assay using an aldehyde reactive probe (ARP). It is shown that oxidative pyrimidine lesions can be also quantitated by the ARP assay in combination with the treatment with endonuclease III.
Kurisu
S
S
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima 739-8526, Japan.
Miya
T
T
Terato
H
H
Masaoka
A
A
Ohyama
Y
Y
Kubo
K
K
Ide
H
H
D000447:Aldehydes / Q000032:analysis* / Q000737:chemistry; D019921:Biotinylation; D004247:DNA / Q000737:chemistry; D004249:DNA Damage; D043224:Deoxyribonuclease (Pyrimidine Dimer); D004706:Endodeoxyribonucleases; D004797:Enzyme-Linked Immunosorbent Assay; D029968:Escherichia coli Proteins; D006367:HeLa Cells; D006801:Humans; D008163:Luminescent Measurements; D015335:Molecular Probes / Q000737:chemistry*
D000447:Aldehydes; D029968:Escherichia coli Proteins; D015335:Molecular Probes; D004247:DNA; D004706:Endodeoxyribonucleases; D043224:Deoxyribonuclease (Pyrimidine Dimer); C106403:NTH protein, E coli