A selective medium for quantitative reisolation of Trichoderma harzianum from Agaricus bisporus compost.
We adapted a selective medium, previously developed for reisolation of Trichoderma spp. from soil, for quantitative determination of growth of T. harzianum from commercial Agaricus bisporus composts. This medium enables comparisons of aggressive (sensu inhibition of A. bisporus yield) with nonaggressive T. harzianum groups. The resulting medium contains the antimicrobials chloramphenicol, streptomycin, quintozene, and propamocarb and was highly selective, allowing the recovery of T. harzianum, as viable conidia and hyphal fragments, in compact colonies with the absence of visible microbial contaminants.
Williams
Josie
J
Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, United Kingdom.
Clarkson
John M
JM
Mills
Peter R
PR
Cooper
Richard M
RM
D000364:Agaricus / Q000254:growth & development* / Q000378:metabolism*; D000935:Antifungal Agents / Q000494:pharmacology; D003470:Culture Media; D009172:Mycology / Q000379:methods; D012988:Soil Microbiology; D014242:Trichoderma / Q000201:enzymology* / Q000254:growth & development* / Q000302:isolation & purification
D000935:Antifungal Agents; D003470:Culture Media