A simple and rapid system for the quantitation of RNA interference in plant cultured cells.
The phenomenon known as RNA interference (RNAi) by double-stranded RNA (dsRNA) that was reported recently in the nematode Caenorhabditis elegans has been shown to operate by a mechanism that is widely conserved among species including plant cells. No quantitative analysis of the effects of RNAi on the expression of specific genes in plant cultured cells has been reported. An RNAi effect was observed 24 h after the introduction of dsRNA expression plasmids into tobacco BY-2 cells by electroporation. The simple system for suppression of specific genes in plant cells should be useful in attempts to elucidate the roles of individual genes in plant cells.
Akashi
H
H
Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Hongo, Tokyo 113-8656, Japan.
Miyagishi
M
M
Kurata
H
H
Nagata
T
T
Taira
K
K
D002460:Cell Line; D018274:Electroporation; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008957:Models, Genetic; D010957:Plasmids / Q000235:genetics; D034622:RNA Interference; D013997:Time Factors; D014026:Nicotiana / Q000235:genetics*
D008156:Luciferases