title: Direct analysis of drugs in plasma by column-switching liquid chromatography-mass spectrometry using a methylcellulose-immobilized reversed-phase pretreatment column.
abstract: A novel methylcellulose-immobilized reversed-phase pretreatment column (MC-ODS) for column switching liquid chromatography-mass spectrometry (LC-MS) was investigated to improve recovery and durability. Pretreatment and analytical conditions were optimized so that high throughput and high selectivity was ensured during mass spectrometric analysis. Analytical runs, including deproteinization and gradient LC analysis, were conducted in a 6-min cycle. As a consequence, recoveries for test drugs (metoprolol, propranolol, lidocaine, dibucaine, bupivacaine) were greater than 90% and more than 300 plasma samples spiked with target compounds were directly injected and measured without compromising MS detection or system performance.
authors.affiliation: Analytical Applications Department, Shimadzu Corporation, 380-1 Horiyamashita, Hadano, Kanagawa 259-1304, Japan.
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Kawano
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Murakita
authors.forename: Eiichi
authors.initials: E
authors.lastname: Yamamoto
authors.forename: Naoki
authors.initials: N
authors.lastname: Asakawa
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000097:Acetonitriles; D004364:Pharmaceutical Preparations; C032159:acetonitrile