Stereospecific analysis of loxoprofen in plasma by chiral column liquid chromatography with a circular dichroism-based detector.
The chiral separation of loxoprofen was achieved on a chiral column with UV and circular dichroism (CD) detection. The good resolution of four loxoprofen stereoisomers was obtained. The column used for the chiral separation was Chiralcel OJ column (250 x 4.6 mm) using hexane-2-propanol-trifluoroacetic acid (95:5:0.1), as an eluent. The flow-rate was 1.0 ml/min and the detection was at 225 nm. In addition, CD and UV spectra were obtained by stopped flow scanning. The method allows the determination of the stereoisomers of loxoprofen in human plasma after the administration of therapeutic dose of the racemic drug, thus HPLC with CD detector is useful for the stereospecific determination of loxoprofen products in biological samples.
Kanazawa
Hideko
H
Kyoritsu College of Pharmacy, Shibakoen 1-5-30, Minato-ku, Tokyo 105-8512, Japan. kanazawa-hd@kyoritsu-ph.ac.jp
Tsubayashi
Akane
A
Nagata
Yoshiko
Y
Matsushima
Yoshikazu
Y
Mori
Chiharu
C
Kizu
Junko
J
Higaki
Megumu
M
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis / Q000097:blood*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D006801:Humans; D007202:Indicators and Reagents; D010666:Phenylpropionates / Q000032:analysis / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D013607:Tablets
D000894:Anti-Inflammatory Agents, Non-Steroidal; D007202:Indicators and Reagents; D010666:Phenylpropionates; D013607:Tablets; C040656:loxoprofen