title: Crystallization and preliminary X-ray analysis of Yersinia pseudotuberculosis-derived mitogen.
abstract: Yersinia pseudotuberculosis-derived mitogen (YPM), a superantigen with no amino-acid sequence similarity to other known superantigens, has been crystallized by the sitting-drop vapour-diffusion method. The crystals belong to space group C2, with unit-cell parameters a = 138.67, b = 78.66, c = 32.91 A, beta = 91.97 degrees. A native data set has been collected to a resolution of 1.8 A using synchrotron radiation. Self-rotation function calculations suggest the presence of three molecules in the asymmetric unit, corresponding to a solvent content of 45%.
authors.affiliation: School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia.
authors.forename: Roberta
authors.initials: R
authors.lastname: Donadini
authors.forename: Malin
authors.initials: M
authors.lastname: Wåhlberg
authors.forename: Takao
authors.initials: T
authors.lastname: Kohsaka
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Ito
authors.forename: Barry A
authors.initials: BA
authors.lastname: Fields
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D003001:Cloning, Molecular; D003460:Crystallization; D004926:Escherichia coli / Q000235:genetics; D008934:Mitogens / Q000737:chemistry*; D011993:Recombinant Fusion Proteins; D012645:Selenomethionine; D018089:Superantigens / Q000737:chemistry; D014961:X-Ray Diffraction; D015011:Yersinia pseudotuberculosis / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D008934:Mitogens; D011993:Recombinant Fusion Proteins; D018089:Superantigens; C093313:Yersinia pseudotuberculosis-derived mitogen; D012645:Selenomethionine