title: Identification of tumor-associated antigens by screening phage-displayed human cDNA libraries with sera from tumor patients.
abstract: Screening cDNA libraries from solid human tumors with sera of autologous patients (SEREX) has proven to be a powerful approach to identifying tumor antigens recognized by the humoral arm of the immune system. In many cases, application of this methodology has led to the discovery of novel tumor antigens as unknown gene products. We tried to improve the potency of the SEREX approach by combining it with phage-display technology. We designed a new lambda vector to express protein fragments as N-terminal fusions to the D capsid protein and generated high-complexity cDNA libraries from human breast carcinoma cell lines and solid tumors. Screening these phage-displayed libraries required limited amounts of sera from patients and efficiently identified several tumor antigens specifically reacting with sera from breast cancer patients.
authors.affiliation: Kenton Labs, c/o Sigma Tau, Pomezia (Roma), Italy.
authors.forename: Olga
authors.initials: O
authors.lastname: Minenkova
authors.affiliation: Kenton Labs, c/o Sigma Tau, Pomezia (Roma), Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Pucci
authors.affiliation: Kenton Labs, c/o Sigma Tau, Pomezia (Roma), Italy.
authors.forename: Emiliano
authors.initials: E
authors.lastname: Pavoni
authors.affiliation: Kenton Labs, c/o Sigma Tau, Pomezia (Roma), Italy.
authors.forename: Amedeo
authors.initials: A
authors.lastname: De Tomassi
authors.affiliation: Kenton Labs, c/o Sigma Tau, Pomezia (Roma), Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Fortugno
authors.affiliation: Kenton Labs, c/o Sigma Tau, Pomezia (Roma), Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Gargano
authors.affiliation: Laboratorio di Immunologia, Reparto Immunologia dei Tumori, Istituto Superiore di Sanità, Roma, Italy.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Cianfriglia
authors.affiliation: Laboratorio di Immunologia, Reparto Immunologia dei Tumori, Istituto Superiore di Sanità, Roma, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Barca
authors.affiliation: Dipartimento di Endocrinologia ed Oncologia Molecolare e Clinica, Università di Napoli Federico II, Napoli, Italy.
authors.forename: Sabino
authors.initials: S
authors.lastname: De Placido
authors.affiliation: Dipartimento di Endocrinologia ed Oncologia Molecolare e Clinica, Università di Napoli Federico II, Napoli, Italy.
authors.forename: Angelo
authors.initials: A
authors.lastname: Martignetti
authors.affiliation: Kenton Labs, c/o Sigma Tau, Pomezia (Roma), Italy.
authors.forename: Franco
authors.initials: F
authors.lastname: Felici
authors.affiliation: Istituto di Ricerche di Biologia Molecolare P. Angeletti, Pomezia (Roma), Italy.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Cortese
authors.affiliation: Istituto di Ricerche di Biologia Molecolare P. Angeletti, Pomezia (Roma), Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Monaci
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D000912:Antibodies, Neoplasm / Q000097:blood; D000951:Antigens, Neoplasm / Q000032:analysis*; D017104:Bacteriophage M13 / Q000235:genetics; D010582:Bacteriophage lambda; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000235:genetics / Q000276:immunology*; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D004273:DNA, Neoplasm / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D015723:Gene Library; D005822:Genetic Vectors; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D008297:Male; D008403:Mass Screening; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D012333:RNA, Messenger / Q000096:biosynthesis; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D017931:DNA Primers; D004273:DNA, Neoplasm; D012333:RNA, Messenger; D005982:Glutathione Transferase