[Targeted therapy of human laryngeal squamous cell carcinoma in vitro with telomerase catalytic subunit mRNA].
OBJECTIVE
To study the effect of antisense oligonucleotide targeted human telomerase catalytic subunit (hTERT) on Hep-2 cell line.


METHODS
Cells were treated daily with oligonucleotide (ODN) directed against hTERT, or control ODN-a mismatched sequence or only Lipofectamine. At 24 h, 48 h, 72 h cell viability after administration of ODNs was determined using the MTT assay. At 72 h, the morpholog alteration of the cells was observed by HE stain. At 72 h, telomerase activity was determined.


RESULTS
After daily transfection ASODN, some Hep-2 cells begin to stop growing up, but not in the mismatched ODN group or Lipofectamine group. Hep-2 cells treated with antisense against hTERT showed a decrease in telomerase activity.


CONCLUSIONS
hTERT antisense ODN can inhibit telomerase activity and the growth of Hep-2 cells in short term.
Chen
Shiming
S
Department of Otolaryngology-Head and Neck Surgery, People's Hospital of Wuhan University, Wuhan 430060.
Tao
Zezhang
Z
Xiao
Bokui
B
Liu
Jianfeng
J
Hou
Wei
W
Yang
Zhanqiu
Z
D004268:DNA-Binding Proteins; D006801:Humans; D007822:Laryngeal Neoplasms / Q000201:enzymology*; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D019098:Telomerase / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured
D004268:DNA-Binding Proteins; D020319:Oligodeoxyribonucleotides, Antisense; D019098:Telomerase