Non-radioactive hybridization in microwells using enzyme linked immune sorbent assay for detection of RT-PCR-amplified CK19- and CEA-mRNA.
PCR enzyme linked immune sorbent assay (ELISA) was developed for detection of RT-PCR-amplified cytokeratin 19 (CK19) mRNA and carcinoembryonic antigen (CEA) mRNA. The non-radioactive hybridization was performed in a streptavidin-coated microwell with digoxigenin-labeled PCR products and with biotin-labeled capture probe. PCR ELISA was proved to be expedient, simple, sensitive and safe for identification of CK19-, CEA-RT-PCR products. These results were proven by sequencing.
Zhong
X
X
Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical University, Wuhan 430030.
Kaul
S
S
Eichler
A
A
Bastert
G
G
D001854:Bone Marrow Cells / Q000737:chemistry / Q000473:pathology; D001943:Breast Neoplasms / Q000737:chemistry / Q000473:pathology*; D002272:Carcinoembryonic Antigen / Q000032:analysis* / Q000235:genetics; D004076:Digoxigenin; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007633:Keratins / Q000032:analysis* / Q000235:genetics; D009360:Neoplastic Cells, Circulating; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D002272:Carcinoembryonic Antigen; D012333:RNA, Messenger; D007633:Keratins; D004076:Digoxigenin