title: Antioxidative glycosides from the leaves of Ligustrum robustum.
abstract: Phytochemical investigation of the ethanol extract of the leaves of Ligustrum robustum, monitored by a bioassay involving the hemolysis of red blood cells induced by 2,2'-azo-bis(2-amidinopropane) dihydrochloride, led to the isolation of three new glycosides, ligurobustosides M (1), N (2), and O (3), along with 10 known ones, osmanthuside B (4), osmanthuside B6 (5), acteoside (6), ligupurpuroside A (7), ligupurpuroside B (8), ligurobustoside C (9), ligurobustoside E (10), ligurobustoside I (11), cosmosiin (12), and rhoifolin (13). The structures of the new compounds were elucidated by spectroscopic methods. Seven of the glycosides showed stronger antioxidant effects than the standard, trolox.
authors.affiliation: Department of Biology, The Chinese University of Hong Kong, Shatin, Hong Kong, People's Republic of China.
authors.forename: Zhen-Dan
authors.initials: ZD
authors.lastname: He
authors.forename: Kit-Man
authors.initials: KM
authors.lastname: Lau
authors.forename: Paul Pui-Hay
authors.initials: PP
authors.lastname: But
authors.forename: Ren-Wang
authors.initials: RW
authors.lastname: Jiang
authors.forename: Hui
authors.initials: H
authors.lastname: Dong
authors.forename: Shuang-Cheng
authors.initials: SC
authors.lastname: Ma
authors.forename: Kwok-Pui
authors.initials: KP
authors.lastname: Fung
authors.forename: Wen-Cai
authors.initials: WC
authors.lastname: Ye
authors.forename: Han-Dong
authors.initials: HD
authors.lastname: Sun
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002839:Chromans / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006461:Hemolysis / Q000187:drug effects; D031659:Ligustrum / Q000737:chemistry*; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats
chemical_list: D000975:Antioxidants; D002839:Chromans; D004365:Drugs, Chinese Herbal; D006027:Glycosides; C478725:ligurobustoside M; C478726:ligurobustoside N; C478727:ligurobustoside O; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid