title: Evaluation of fungal burden in experimental paracoccidioidomycosis by using the fluorescent dye Blankophor.
abstract: The fungal load in organs and blood of susceptible and resistant mice infected with Paracoccidioides brasiliensis was quantitated by using the optical brightener Blankophor and compared with CFU counts. Fluorescent staining of fungal cells proved to be a quick and easy procedure, suitable for evaluation of paracoccidioidomycotic infection.
authors.affiliation: Departamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Angela Satie
authors.initials: AS
authors.lastname: Nishikaku
authors.forename: Eva
authors.initials: E
authors.lastname: Burger
mesh_terms: D000818:Animals; D001557:Benzenesulfonates / Q000378:metabolism*; D001769:Blood / Q000382:microbiology; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D005456:Fluorescent Dyes / Q000378:metabolism*; D016469:Fungemia / Q000382:microbiology; D006801:Humans; D051379:Mice; D009852:Omentum / Q000382:microbiology; D010179:Pancreas / Q000382:microbiology; D010228:Paracoccidioides / Q000302:isolation & purification* / Q000472:pathogenicity; D010229:Paracoccidioidomycosis / Q000382:microbiology* / Q000503:physiopathology; D013154:Spleen / Q000382:microbiology; D014930:Women
chemical_list: D001557:Benzenesulfonates; D005456:Fluorescent Dyes; C414926:Blankophore P fluessig