title: Lymphocyte subset distribution and cytokine secretion in third trimester decidua in normal pregnancy and preeclampsia.
abstract: BACKGROUND
  Excessive Th1 activity in peripheral blood plays a probable role in the pathogenesis of preeclampsia. The aim of the study was to investigate whether disturbed local immune reactions are also present in decidua.
  
  
  METHODS
  Flow cytometric analysis of CD3, CD19, CD56/CD16, CD4, CD8, CD4/CD29, CD4/CD45RA, CD4/CD45RO, CD8/CD28, CD3/CD69 lymphocyte subsets isolated from third trimester decidua of pregnants with preeclampsia (n=21) and pregnant controls (n=11) subjected to elective caesarean sections. Spontaneous and phytohemaglutynine stimulated "in vitro" secretion of IL-2, IL-4, IL-6, IL-10, IL-12, IFN-gamma and TGF-beta by decidual lymphocytes was studied by ELISA. For the statistical significance of differences between the groups the U Mann-Whitney test was performed (confidence interval P<0.05).
  
  
  RESULTS
  Preeclamptic patients were characterized with an increased percentage of the CD3-/CD56+CD16+, CD8+/CD28+ and decreased percentage of CD3+, CD19+, CD4+/CD45RA+ lymphocytes. The profile of secreted cytokines shifts in favor of Th1 activity (extremely high IFN-gamma and low IL-6 and IL-10 secretion). Decidual IL-12 secretion in preeclamptic patients is decreased compared to controls.
  
  
  CONCLUSIONS
  Changes in NK and T lymphocyte subsets followed with Th1 cytokine IFN-gamma over-activity, could affect local immunoregulatory mechanisms in third trimester decidua of preeclamptic patients.
authors.affiliation: Department of Obstetrics and Gynecology, Military Medical Academy, Lodz, Poland. jrwil@post.pl
authors.forename: Jacek R
authors.initials: JR
authors.lastname: Wilczyński
authors.forename: Henryk
authors.initials: H
authors.lastname: Tchórzewski
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Banasik
authors.forename: Ewa
authors.initials: E
authors.lastname: Głowacka
authors.forename: Artur
authors.initials: A
authors.lastname: Wieczorek
authors.forename: Przemysław
authors.initials: P
authors.lastname: Lewkowicz
authors.forename: Andrzej
authors.initials: A
authors.lastname: Malinowski
authors.forename: Marian
authors.initials: M
authors.lastname: Szpakowski
authors.forename: Jan
authors.initials: J
authors.lastname: Wilczyński
mesh_terms: D015703:Antigens, CD / Q000032:analysis; D016207:Cytokines / Q000378:metabolism*; D003656:Decidua / Q000276:immunology* / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D007694:Killer Cells, Natural / Q000276:immunology / Q000473:pathology; D016131:Lymphocyte Subsets / Q000473:pathology*; D008214:Lymphocytes / Q000276:immunology; D011225:Pre-Eclampsia / Q000276:immunology* / Q000473:pathology; D011247:Pregnancy; D011263:Pregnancy Trimester, Third; D012016:Reference Values; D018417:Th1 Cells / Q000276:immunology; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D016207:Cytokines; D007376:Interleukin-2; D015850:Interleukin-6; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D018664:Interleukin-12; D015847:Interleukin-4; D007371:Interferon-gamma