ECM homeostasis in renal diseases: a genomic approach.
Chronic renal disease is in general histologically accompanied by a vast amount of scar tissue, ie glomerulosclerosis and interstitial fibrosis. Scarring results from excessive accumulation of extracellular matrix (ECM) components, a process driven by a plethora of cytokines and growth factors. Studies in experimental renal disease which target these regulators using gene therapy limit or prevent the development of scarring. This review focuses specifically on the role of transforming growth factor-beta, platelet-derived growth factor, connective tissue growth factor, hepatocyte growth factor, and epidermal growth factor. The results obtained in animal models hold promise for molecular intervention strategies in human renal disease. Microarray technology allows large-scale gene expression profiling in kidney tissue to identify common molecular pathways in a step towards discovery of new drug targets. Molecular techniques are expected to be used for diagnostic and prognostic purposes in nephrological practice to supplement renal biopsy. Several studies already show that molecular techniques might be of use in routine diagnostic practice. Improvement of diagnosis and prediction of outcome in renal patients might lead to more efficient and earlier therapeutic intervention.
Eikmans
M
M
Department of Pathology, Leiden University Medical Center, Building 1, LI-Q, PO Box 9600, 2300 RC Leiden, The Netherlands. M.Eikmans@LUMC.NL
Baelde
J J
JJ
de Heer
E
E
Bruijn
J A
JA
D016207:Cytokines / Q000378:metabolism*; D005109:Extracellular Matrix / Q000378:metabolism*; D005355:Fibrosis; D020869:Gene Expression Profiling; D006133:Growth Substances / Q000378:metabolism*; D006706:Homeostasis; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D007674:Kidney Diseases / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*
D016207:Cytokines; D006133:Growth Substances