Expression of endothelin-1 in gingival epithelial cells.
OBJECTIVE
Endothelin-1 (ET-1) is a 21-amino acid peptide with multifunctional regulation. ET-1 expresses in various cells during inflammation. The present study aimed to examine the ET-1 expression in oral epithelial cells after infection with the periodontal pathogen and to investigate the presence of ET-1 in human inflamed and uninflamed gingival tissues.


METHODS
The KB cells were infected with Porphyromonas gingivalis and the expression level of ET-1 was examined using reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). The immunohistochemical analysis of ET-1 was performed in gingival tissues obtained from patients. In addition, the ET-1 mRNA expression in each tissue was also investigated by RT-PCR.


RESULTS
The expression of ET-1 in KB cells was strongly induced by the P. gingivalis infection. On the other hand, the strong immunoreactivity for ET-1 was observed in the epithelium and vascular endothelial cells of the inflamed gingival tissue. Furthermore, the level of ET-1 mRNA was greater in the inflamed tissues.


CONCLUSIONS
These results suggested that the expression level of ET-1 in gingival epithelial cells might be enhanced during the periodontal inflammation.
Yamamoto
Eri
E
Department of Preventive Dentistry, Kyushu Dental College, Kitakyushu, Japan.
Awano
Shuji
S
Koseki
Takeyoshi
T
Ansai
Toshihiro
T
Takehara
Tadamichi
T
D000328:Adult; D000368:Aged; D016866:Bacteroidaceae Infections / Q000378:metabolism; D019332:Endothelin-1 / Q000032:analysis* / Q000235:genetics; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D005881:Gingiva / Q000166:cytology / Q000378:metabolism*; D005884:Gingival Hemorrhage / Q000378:metabolism / Q000473:pathology; D005891:Gingivitis / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007624:KB Cells / Q000378:metabolism / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D010514:Periodontal Pocket / Q000378:metabolism / Q000473:pathology; D016966:Porphyromonas gingivalis / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D015854:Up-Regulation
D019332:Endothelin-1; D012333:RNA, Messenger