Knockout mice as model systems for studying nm23/NDP kinase gene functions. Application to the nm23-M1 gene.
Mice carrying a homozygous germ-line mutation in the nm23-M1 gene that eliminates its protein expression and drives expression of beta-galactosidase by nm23-M1 promoter have been generated. nm23-M1 gene inactivation is not teratogenic and the pups can grow to adult age without apparent health problems. However, they undergo a growth retardation and knocked out females cannot feed their pups. Both effects are background dependent. Beta-galactosidase mapping of nm23-M1 promoter activation during embryogenesis shows that the nm23-M1 gene is principally expressed in epithelial layer of tissues which require inductive epithelial-mesenchymal interactions for their formation. In conclusion, invalidated mice could be interesting models to analyze the role of nm23-M1 on signal transduction pathway regulation, or cancer induction and proliferation.
Arnaud-Dabernat
S
S
Biologie de la Différenciation et du Développement, Université Victor Segalen-Bordeaux2, Bordeaux, France.
Bourbon
P M
PM
Dierich
A
A
Le Meur
M
M
Daniel
J Y
JY
D000818:Animals; D000831:Animals, Newborn; D001940:Breast / Q000378:metabolism*; D003001:Cloning, Molecular; D005260:Female; D005317:Fetal Growth Retardation / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D051379:Mice; D018345:Mice, Knockout / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D023421:Models, Animal; D054778:NM23 Nucleoside Diphosphate Kinases; D009701:Nucleoside-Diphosphate Kinase; D011506:Proteins / Q000235:genetics* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D013329:Structure-Activity Relationship
D054778:NM23 Nucleoside Diphosphate Kinases; D011506:Proteins; D011994:Recombinant Proteins; C517939:Nme1 protein, mouse; D009701:Nucleoside-Diphosphate Kinase