Comparison of tamarins and marmosets as hosts for GBV-B infections and the effect of immunosuppression on duration of viremia.
GBV-B virus is a close relative to hepatitis C virus (HCV) that causes hepatitis in tamarins, and thus, is an attractive surrogate model for HCV. In this study, we demonstrate that the host range of GBV-B extends to the common marmoset with an infection profile similar to that observed for tamarins. Marmoset hepatocytes were susceptible to in vitro infection with GBV-B. Virus was efficiently secreted into the medium, and approximately 25% of hepatocytes were positive for NS3 staining. In an attempt to induce persistent infections, tamarins were immunosuppressed with FK506 and inoculated with GBV-B. Although no chronic infections were induced, the duration of viremia was increased in most animals. In one animal, the duration of viremia was extended to 46 weeks, but viral clearance occurred 18 weeks after stopping FK506 therapy. The greater availability of marmosets in comparison to tamarins will greatly facilitate future research efforts with this model.
Lanford
Robert E
RE
Department of Virology and Immunology, Southwest National Primate Research Center, and Southwest Foundation for Biomedical Research, San Antonio, TX 78227, USA. rlanford@icarus.sfbr.org
Chavez
Deborah
D
Notvall
Lena
L
Brasky
Kathleen M
KM
D000818:Animals; D002144:Callithrix; D002478:Cells, Cultured; D004195:Disease Models, Animal; D018178:Flaviviridae Infections / Q000276:immunology / Q000662:veterinary*; D029406:GB virus B; D006524:Hepatitis, Viral, Animal / Q000276:immunology / Q000821:virology*; D022781:Hepatocytes / Q000821:virology; D016867:Immunocompromised Host; D007166:Immunosuppressive Agents; D008992:Monkey Diseases / Q000276:immunology / Q000821:virology*; D012452:Saguinus; D016559:Tacrolimus; D013997:Time Factors; D014766:Viremia
D007166:Immunosuppressive Agents; D016559:Tacrolimus