title: Projections from the raphe nuclei to the suprachiasmatic nucleus of the rat.
abstract: The presence of serotonergic afferents in the hypothalamic suprachiasmatic nucleus (SCN) is well documented and several functional roles of serotonin (5-HT) in circadian function are well established. However, there is some controversy about the precise location of the serotonergic neurones from where this input arises. Discrete injection of the tracer Cholera toxin, subunit B, (ChB) was centred in the rat SCN, and a few retrograde labelled neurones were distributed in the dorsal and median raphe nuclei (MnR) and in the rostral part of the raphe magnus (RMg), but no neurones were found in the raphe pallidus or raphe obscurus. In addition, a group of neurones was consistently found in the medial part of the pontine supra lemniscal nucleus but not including the serotonergic B(9) region. A combination of retrograde tracing with Fluoro-Gold together with 5-HT-immunolabelling, showed few double-labelled neurones in the dorsal, MnR and B(9). However, the majority of projecting neurones were not co-storing 5-HT immunoreactivity. Phaseolus vulgaris-leucoagglutinin (PHA-L) injections in the dorsal raphe resulted in faint labelling, whereas the MnR gave rise to several labelled fibres in the SCN. Individual delicate PHA-L nerve fibres were found in all compartments of the SCN both in terms of rostrocaudal, ventromedial and dorsomedial extent, without any sign of a topographical organisation of the MnR input to the SCN. PHA-L injections into RMg gave rise to labelling of a few processes within the SCN. In conclusion, the main serotonergic input to the rat SCN originates from a few neurones in the MnR.
authors.affiliation: Department of Medical Anatomy, The Panum Institute, 18.2, University of Copenhagen, Blegdamsvej 3, DK-2200 N, Copenhagen, Denmark. a.hay@mai.ku.dk
authors.forename: Anders
authors.initials: A
authors.lastname: Hay-Schmidt
authors.forename: Niels
authors.initials: N
authors.lastname: Vrang
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Larsen
authors.forename: Jens D
authors.initials: JD
authors.lastname: Mikkelsen
mesh_terms: D000818:Animals; D002940:Circadian Rhythm; D007150:Immunohistochemistry; D008297:Male; D009434:Neural Pathways / Q000166:cytology*; D009475:Neurons, Afferent / Q000166:cytology*; D011903:Raphe Nuclei / Q000033:anatomy & histology* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000378:metabolism; D013493:Suprachiasmatic Nucleus / Q000033:anatomy & histology* / Q000378:metabolism
chemical_list: D012701:Serotonin