Kinetic analyses of ATP-dependent deoxyribonuclease (DNase) reactions on a quartz-crystal microbalance.
We report here kinetic analyses of the hydrolysis of DNA by the ATP-dependent DNase using a DNA-immobilized quartz-crystal microbalance (QCM), which enables in situ real-time monitoring both the binding of enzyme and the hydrolysis reaction on DNA strands, as mass changes.
Matsuno
H
H
Department of Biomolecular Engineering, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.
Furusawa
H
H
Okahata
Y
Y
D000255:Adenosine Triphosphate / Q000378:metabolism*; D001483:Base Sequence; D003460:Crystallization; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D008969:Molecular Sequence Data; D011485:Protein Binding; D011791:Quartz / Q000737:chemistry*
D011791:Quartz; D000255:Adenosine Triphosphate; D004247:DNA; D003851:Deoxyribonucleases