Truncated Bcl-2, a potential pre-metastatic marker in prostate cancer.
A novel truncated form of Bcl-2, termed Bcl-2psi, was discovered in invasive prostate cancer cells, using laser capture microdissection, RNA-polymerase cycling reaction, and microarray analysis. The expression of Bcl-2psi increased prior to metastasis in higher-grade prostate cancer. The immunoreactive Bcl-2psi was specifically identified in higher-grade prostate cancer cells. These findings suggest that Bcl-2psi may be a potential pre-metastatic marker for detection, diagnosis, and therapy during the initiation of metastasis in prostate cancer.
Huang
Jen-Ming
JM
Department of Cell and Neurobiology, Keck School of Medicine, University of Southern California, BMT-401, 1333 San Pablo Street, Los Angeles, CA 90033, USA.
Lin
Tsen-Yin
TY
Chang
Donald
D
Lin
Shi-Lung
SL
Ying
Shao-Yao
SY
D001483:Base Sequence; D014408:Biomarkers, Tumor; D015152:Blotting, Northern; D015153:Blotting, Western; D004210:Dissection; D004591:Electrophoresis, Polyacrylamide Gel; D015723:Gene Library; D006801:Humans; D007150:Immunohistochemistry; D007834:Lasers; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation; D009362:Neoplasm Metastasis; D020411:Oligonucleotide Array Sequence Analysis; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000737:chemistry*; D012313:RNA / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D014407:Tumor Cells, Cultured
C476231:Bcl-2psi protein, human; D014408:Biomarkers, Tumor; D019253:Proto-Oncogene Proteins c-bcl-2; D012313:RNA