The determination of immune reactive proteins in Cysticercus tenuicollis cyst fluids by SDS-PAGE and western blotting in sheep.
Cysticercus tenuicollis, the larva of Taenia hydatigena, is commonly seen in sheep and goats slaughtered in Turkey. In this study, the protein bands were revealed in C. tenuicollis cyst fluid antigen by using SDS-PAGE and Western blotting, and immune reactive bands were determined. Ten positive and 10 negative sera for C. tenuicollis from sheep and one non-infected sheep serum were tested in this experiment. According to the results, there was only one protein band determined to be immune reactive, which was 36 kDa.
Kara
M
M
Department of Parasitology, Faculty of Veterinary Medicine, Kafkas University, 36100 Kars, Turkey. muratkara96@yahoo.com
Sarimehmetoglu
H O
HO
Gonenc
B
B
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis / Q000737:chemistry / Q000276:immunology*; D015153:Blotting, Western / Q000662:veterinary; D003551:Cysticercosis / Q000175:diagnosis / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D003552:Cysticercus / Q000276:immunology* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D015801:Helminth Proteins / Q000032:analysis / Q000737:chemistry / Q000276:immunology*; D008970:Molecular Weight; D012680:Sensitivity and Specificity; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis / Q000276:immunology / Q000469:parasitology*
D000947:Antigens, Helminth; D015801:Helminth Proteins