Alteration of protein constituents induced by low-energy (<35 eV) electrons: II. Dissociative electron attachment to amino acids containing cyclic groups.
We report measurements of the desorption of anions from thin condensed films of tryptophan (Trp), histidine (His) and proline (Pro) stimulated by 5-35 eV electron impact. H-, O-, OH- and CN- desorb from Trp, His and Pro, whereas CH2- is observed only from Pro fragmentation. Below 12 eV, the anion yield functions exhibit resonant structures indicative of dissociative electron attachment. For all three amino acids, this process is likely to be initiated by the resonant capture of the incident electron at the NH3(+)-CH-.....-COO- and/or NH2-CH-.....-COOH group of the molecule. Temporary electron attachment to the ring leads to anion desorption only for tryptophan and proline. The energy-averaged yields measured at the detector of the mass spectrometer are (4.9, 0.3 and 54.0) x 10(-8) H-/incident electron and (3.4, 2.9, 1.8) x 10(-11) O-/incident electron, respectively, from Trp, His and Pro dissociation. Fragmentation of amino acids is found to be as intense as that of the nucleic acid bases. These results are discussed within the context of radiobiological damage induced by secondary electrons.
Abdoul-Carime
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Groupe des Instituts de Recherche en Santé du Canada en Sciences des Radiations, Faculté de Médecine, Université de Sherbrooke, Sherbrooke, Québec, Canada J1H 5N4.
Sanche
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D000596:Amino Acids / Q000737:chemistry; D000838:Anions; D004583:Electrons; D006639:Histidine / Q000737:chemistry; D013058:Mass Spectrometry; D008956:Models, Chemical; D010455:Peptides / Q000528:radiation effects*; D011392:Proline / Q000737:chemistry; D013696:Temperature; D014364:Tryptophan / Q000737:chemistry
D000596:Amino Acids; D000838:Anions; D010455:Peptides; D006639:Histidine; D014364:Tryptophan; D011392:Proline