Expression of Bcl-2 on leukocytes in prostatic fluid from patients with acute and chronic prostatitis.
In our continuing investigation to clarify the significance of leukocytosis and its prolongation in prostatic fluid (PF) from prostatitis patients, we investigated whether expression of the antiapoptotic gene Bcl-2 on leukocytes in prostatic fluid has any relation with prolonged leukocytosis in prostatitis. The subjects were eight patients with acute bacterial prostatitis (ABP) and eight with nonbacterial prostatitis (NBP) with a history of more than 2 years. Twelve micrograms of protein obtained from leukocytes in PF were subjected to Western blot analysis. Bcl-2 was expressed in seven of eight (87.5%) and six of eight (75.0%) patients with NBP and ABP, respectively. Quantitative density analysis of the Bcl-2 band did not show any statistically significant differences between patients with NBP and those with ABP. These results suggest that the expression of antiapoptotic Bcl-2 on leukocytes in PF is not a cause of leukocyte longevity in long-term prostatitis.
Abe
Hiroyuki
H
Department of Urology, Nippon Medical School, Tokyo, Japan.
Nishimura
Taiji
T
Asoh
Sadamitsu
S
Ohta
Shigeo
S
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D017209:Apoptosis; D002908:Chronic Disease; D006801:Humans; D007962:Leukocytes / Q000737:chemistry / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000502:physiology; D011467:Prostate / Q000737:chemistry*; D011472:Prostatitis / Q000097:blood*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000097:blood*
D019253:Proto-Oncogene Proteins c-bcl-2