title: A new coupling method for the synthesis of oligodeoxyribonucleotides based on protection by protonation of nucleoside bases.
abstract: We developed a conceptually new strategy for the coupling method in the phosphoramidite method without protection of nucleoside bases. This strategy, called "proton-block method", significantly reduced side reactions compared with the previous methods.
authors.affiliation: Department of Life Science, Tokyo Institute of Technology, Nagatsuta, Midoriku, Yokohama 226-8501, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Ohkubo
authors.forename: K
authors.initials: K
authors.lastname: Seio
authors.forename: M
authors.initials: M
authors.lastname: Sekine
mesh_terms: D000241:Adenosine / Q000737:chemistry; D002625:Chemistry, Organic / Q000379:methods*; D003562:Cytidine / Q000737:chemistry; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000737:chemistry; D011522:Protons
chemical_list: D009838:Oligodeoxyribonucleotides; D009943:Organophosphorus Compounds; D011522:Protons; C434331:phosphoramidite; D003562:Cytidine; D000241:Adenosine