p53-induced gene expression analysis.
Researchers in the p53 field have successfully used many high-throughput screening technologies to analyze and characterize p53-induced gene expression. This chapter will focus on one such technology, the Affymetrix GeneChip. DNA-Chip technology has grown rapidly over the last several years. The ability to hybridize RNA from a sample to thousands to tens of thousands of known and unknown cDNAs spotted on a microarray chip has led to the explosion of information ranging from macro-global expression pattern changes to micro-gene-specific expression changes. The relative ease of making a nonradioactive probe from either total RNA or mRNA to be hybridized to a GeneChip makes microarray technology highly attractive.
Sax
Joanna K
JK
Howard Hughes Medical Institute, Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, USA.
El-Deiry
Wafik S
WS
D000818:Animals; D018076:DNA, Complementary / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012313:RNA / Q000302:isolation & purification / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
D018076:DNA, Complementary; D016159:Tumor Suppressor Protein p53; D012313:RNA