title: Prevalence of metallo-beta-lactamase among Pseudomonas aeruginosa and Acinetobacter baumannii in a Korean university hospital and comparison of screening methods for detecting metallo-beta-lactamase.
abstract: To identify the metallo-beta-lactamases (MBLs) prevalent in Korea, a total of 130 clinical isolates of Pseudomonas aeruginosa and Acinetobacter baumannii (99 P. aeruginosa and 31 A. baumannii) with a reduced susceptibility to imipenem (IPM) and/or ceftazidime (CAZ) was subjected to PCR analyses with primers specific to bla(IMP-1), bla(VIM-1), and bla(VIM-2). In addition, inhibitor-potentiated disk diffusion methods (IPD) using two kinds of substrate-inhibitor combinations (ceftazidime-2-mercaptopropionic acid (2MPA) and imipenem-EDTA) were investigated. Thirty-three isolates (29 P. aeruginosa and 4 A. baumannii) carried bla(VIM-2) and two P. aeruginosa isolates harbored bla(IMP-1). The enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) pattern revealed that many of the VIM-2-producing P. aeruginosa isolates were clonally related, whereas the A. baumannii isolates were diverse. The inhibitor-potentiated disk diffusion test using imipenem-EDTA was highly sensitive and specific for detecting the VIM-2 producer. These results suggest that VIM-2 is an important MBL in P. aeruginosa and A. baumannii in the Korean hospital of this study and that the IMP-1-producing P. aeruginosa has also emerged. Screening for MBLs and strict infection control for these isolates will contribute to prevent further spread of resistance.
authors.affiliation: Department of Clinical Pathology, College of Medicine, The Catholic University of Korea, Kangnam St. Mary's Hospital, 505 Banpo-dong, Seocho-ku, Seoul 137-040, South Korea.
authors.forename: Eun-Jee
authors.initials: EJ
authors.lastname: Oh
authors.forename: Seungok
authors.initials: S
authors.lastname: Lee
authors.forename: Yeon-Joon
authors.initials: YJ
authors.lastname: Park
authors.forename: Jung Jun
authors.initials: JJ
authors.lastname: Park
authors.forename: Kanggyun
authors.initials: K
authors.lastname: Park
authors.forename: Sang-Il
authors.initials: SI
authors.lastname: Kim
authors.forename: Moon Won
authors.initials: MW
authors.lastname: Kang
authors.forename: Byung Kee
authors.initials: BK
authors.lastname: Kim
mesh_terms: D000150:Acinetobacter / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D000151:Acinetobacter Infections / Q000188:drug therapy / Q000382:microbiology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology / Q000627:therapeutic use; D002442:Ceftazidime / Q000494:pharmacology / Q000627:therapeutic use; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics; D006801:Humans; D015378:Imipenem / Q000494:pharmacology / Q000627:therapeutic use; D007723:Korea; D008826:Microbial Sensitivity Tests / Q000379:methods; D016133:Polymerase Chain Reaction; D011552:Pseudomonas Infections / Q000188:drug therapy / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D012372:ROC Curve; D013438:Sulfhydryl Compounds / Q000494:pharmacology; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D013438:Sulfhydryl Compounds; D015378:Imipenem; D002442:Ceftazidime; C417264:VIM-1 metallo-beta-lactamase; C409201:beta-lactamase IMP-1; D001618:beta-Lactamases; C023884:thiolactic acid