[Rat heart transplantation immune tolerance induced by blockade of B7-CD28 interaction].
OBJECTIVE
To study immune tolerance induced by blockade of B7-CD28 interaction after heart transplantation in rats.


METHODS
A model of rat heterotopic heart allograft was established by using Ono's technique. CTLA-4-Ig was administered by intraperitoneal injection at dose of 100 micro. Survival times, histological changes, and the concentration of IL-2, IgG and IgM were measured after operation.


RESULTS
Survival times were 31.60 +/- 1.82 days in experimental group and vs 7.25 +/- 0.71 days in control groups. In the experimental group, the graft survival time prolonged significantly (P < 0.01). Histological changes were characterized by mild scattered mononuclear infiltration and patchy myocyte injury in the experimental group (grade I - II). In the control group, the changes were characterized by severe lymphocyte infiltration, myocyte injury and necrosis, interstitial edema, and hemorrhage (grade IV). IL-2 was significantly decreased after operation (P < 0.01). IgG and IgM were not changed significantly in comparison with those in the control group (P > 0.05).


CONCLUSIONS
The blockade B7-CD28 interaction can induce rat heterotopic heart allograft immune tolerance. It may provide a new way to treat immune rejection after transplantation.
Qian
Y
Y
Organ Transplantation Centre, First Affiliated Hospital, Sun Yat-sen University of Medical Sciences, Guangzhou, China.
Chen
G
G
Huang
J
J
D000069594:Abatacept; D000818:Animals; D018122:B7-1 Antigen / Q000276:immunology*; D018106:CD28 Antigens / Q000276:immunology*; D004195:Disease Models, Animal; D006084:Graft Rejection / Q000276:immunology / Q000517:prevention & control; D006085:Graft Survival / Q000276:immunology; D016027:Heart Transplantation / Q000276:immunology* / Q000379:methods; D018796:Immunoconjugates / Q000008:administration & dosage / Q000494:pharmacology; D007166:Immunosuppressive Agents / Q000008:administration & dosage / Q000494:pharmacology; D007274:Injections, Intraperitoneal; D009206:Myocardium / Q000276:immunology / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D023001:Transplantation Tolerance / Q000187:drug effects / Q000276:immunology*; D016042:Transplantation, Heterotopic
D018122:B7-1 Antigen; D018106:CD28 Antigens; D018796:Immunoconjugates; D007166:Immunosuppressive Agents; D000069594:Abatacept