title: New role of delta2-glutamate receptors in AMPA receptor trafficking and cerebellar function.
abstract: Previous gene knockout studies have shown that the orphan glutamate receptor delta2 (GluRdelta2) is critically involved in synaptogenesis between parallel fibers and Purkinje cells during development. However, the precise function of GluRdelta2 and whether it is functional in the mature cerebellum remain unclear. To address these issues, we developed an antibody specific for the putative ligand-binding region of GluRdelta2, and application of this antibody to cultured Purkinje cells induced AMPA receptor endocytosis, attenuated synaptic transmission and abrogated long-term depression. Moreover, injection of this antibody into the subarachnoidal supracerebellar space of adult mice caused transient cerebellar dysfunction, such as ataxic gait and poor performance in the rotorod test. These results indicate that GluRdelta2 is involved in AMPA receptor trafficking and cerebellar function in adult mice.
authors.affiliation: Laboratory for Memory & Learning, RIKEN Brain Science Institute, Saitama 351-0198, Japan. hirai@med.kobe-u.ac.jp
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Hirai
authors.forename: Thomas
authors.initials: T
authors.lastname: Launey
authors.forename: Sumiko
authors.initials: S
authors.lastname: Mikawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Torashima
authors.forename: Dai
authors.initials: D
authors.lastname: Yanagihara
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Kasaura
authors.forename: Akihiro
authors.initials: A
authors.lastname: Miyamoto
authors.forename: Michisuke
authors.initials: M
authors.lastname: Yuzaki
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D002478:Cells, Cultured; D002531:Cerebellum / Q000187:drug effects / Q000502:physiology*; D004705:Endocytosis / Q000187:drug effects; D036881:Long-Term Synaptic Depression / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D021381:Protein Transport; D011689:Purkinje Cells / Q000378:metabolism*; D018091:Receptors, AMPA / Q000378:metabolism*; D017470:Receptors, Glutamate / Q000276:immunology / Q000502:physiology*; D009435:Synaptic Transmission / Q000187:drug effects
chemical_list: D000906:Antibodies; D018091:Receptors, AMPA; D017470:Receptors, Glutamate; C107913:glutamate receptor delta 2