Effects of soybean ethanol extract on the cell survival and oxidative stress in osteoblastic cells.
Recent evidence suggests that high concentrations of tumor necrosis factor-alpha (TNF-alpha) and nitric oxide (NO) are thought to increase the apoptosis in osteoblasts and bone resorption and may have important roles in the regulation of osteoblast and osteoclast metabolism, especially in rheumatoid arthritis. The present study was performed to investigate the effect of soybean ethanol extract on the scavenging properties using DPPH and the TNF-alpha and NO production of osteoblastic MC3T3-E1 cells. The soy extract and its fractions according to polarity displayed a strong free radical scavenger activity at 0.01 approximately 0.1g/L, except for aquous fraction which had no significant effect on the function of MC3T3-E1 cells (p < 0.05). TNF-alpha secretion by MC3T3-E1 cells was reduced significantly when stimulated with soy extract (0.05 g/L). Nitrite accumulation in culture medium and apoptosis of MC3T3-E1 cells were induced by the addition of 10(-10) M TNF-alpha, and inhibited by the simultaneous addition of soy extract (0.05g/L).
Choi
Eun Mi
EM
Department of Food and Nutrition, Kyung Hee University, Seoul Korea.
Koo
Sung Ja
SJ
D000469:Alkaline Phosphatase / Q000187:drug effects; D017209:Apoptosis / Q000187:drug effects; D001172:Arthritis, Rheumatoid / Q000188:drug therapy; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D009569:Nitric Oxide / Q000378:metabolism; D010006:Osteoblasts / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy; D010851:Picrates; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D013025:Glycine max; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D000469:Alkaline Phosphatase