Sensitive determination of pethidine in body fluids by gas chromatography-tandem mass spectrometry.
We have presented a simple and sensitive method for determining pethidine, a narcotic analgesic drug in body fluids by gas chromatography-tandem mass spectrometry (GC-MS/MS). Pethidine and 4'-piperidinoacetophenone (internal standard) were extracted from body fluids with Bond Elut C(18) columns; the recoveries were above 85% for both compounds. The calibration curves for blood and urine showed good linearities in the range of 1.25-40 ng/ml. Its detection limits (signal-to-noise ratio=3) were estimated to be approximately 0.5 ng/ml of whole blood and urine.
Ishii
Akira
A
Department of Legal Medicine, Fujita Health University School of Medicine, 1-98 Kutsukake-cho, Toyoake 470-1192, Japan. akishii@fujita-hu.ac.jp
Tanaka
Miwa
M
Kurihara
Rina
R
Watanabe-Suzuki
Kanako
K
Kumazawa
Takeshi
T
Seno
Hiroshi
H
Suzuki
Osamu
O
Katsumata
Yoshinao
Y
D000701:Analgesics, Opioid / Q000097:blood / Q000378:metabolism* / Q000652:urine; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D008614:Meperidine / Q000097:blood / Q000378:metabolism* / Q000652:urine; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000701:Analgesics, Opioid; D008614:Meperidine