title: Expression of endogenous granzyme B in a subset of human primary breast carcinomas.
abstract: Granzyme B (GrB) is the prototypic member of a serine protease family primarily used by cytotoxic lymphocytes to kill target cells. We report here that, by immunohistochemical staining of paraffin-embedded tumour sections, GrB protein was unexpectedly detected in malignant cells of a subset of breast cancers and their adjacent reactive endothelial and mesenchymal cells in which endogenous retinoblastoma protein (pRB) is overexpressed. The identity of the endogenous GrB was further confirmed experimentally in RB-deficient breast carcinoma cell culture upon overexpression of ectopic pRB. Our finding extends the recent paradigm-shifting trend for a more diverse biological role of granzyme B, and might provide a rational basis for exploring its potential prognostic value in a variety of human cancers.
authors.affiliation: Department of Blood and Marrow Transplantation, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
authors.forename: S X
authors.initials: SX
authors.lastname: Hu
authors.forename: S
authors.initials: S
authors.lastname: Wang
authors.forename: J P
authors.initials: JP
authors.lastname: Wang
authors.forename: G B
authors.initials: GB
authors.lastname: Mills
authors.forename: Y
authors.initials: Y
authors.lastname: Zhou
authors.forename: H-J
authors.initials: HJ
authors.lastname: Xu
mesh_terms: D001706:Biopsy; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000201:enzymology* / Q000473:pathology; D002277:Carcinoma / Q000201:enzymology* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D053804:Granzymes; D006801:Humans; D007150:Immunohistochemistry; D012697:Serine Endopeptidases / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured
chemical_list: C509213:GZMB protein, human; D053804:Granzymes; D012697:Serine Endopeptidases