title: Crystallization and preliminary X-ray study of alpha-glucosidase from Geobacillus sp strain HTA-462, one of the deepest sea bacteria.
abstract: An alpha-glucosidase (EC 3.2.1.20) was purified from Geobacillus sp. strain HTA-462 cells and crystallized using the hanging-drop vapour-diffusion technique. The Geobacillus strain is a thermophilic and high-pressure-resistant bacterium found at the bottom of the Challenger Deep in the Mariana Trench. The crystal was characterized by X-ray diffraction and belongs to space group C2, with unit-cell parameters a = 104.0, b = 91.5, c = 72.9 A, beta = 109.4 degrees. Diffraction data to 2.5 A resolution were collected and processed.
authors.affiliation: Department of Computational Biology, Biomolecular Engineering Research Institute, 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan. shirai@beri.or.jp
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Shirai
authors.forename: Vo Si
authors.initials: VS
authors.lastname: Hung
authors.forename: Masatake
authors.initials: M
authors.lastname: Akita
authors.forename: Yuji
authors.initials: Y
authors.lastname: Hatada
authors.forename: Susumu
authors.initials: S
authors.lastname: Ito
authors.forename: Koki
authors.initials: K
authors.lastname: Horikoshi
mesh_terms: D001406:Bacillaceae / Q000201:enzymology*; D003460:Crystallization; D004791:Enzyme Inhibitors / Q000737:chemistry; D005942:Gluconates / Q000737:chemistry; D065089:Glycoside Hydrolase Inhibitors; D008386:Marine Biology; D012623:Seawater / Q000382:microbiology; D014961:X-Ray Diffraction; D000520:alpha-Glucosidases / Q000737:chemistry*
chemical_list: D004791:Enzyme Inhibitors; D005942:Gluconates; D065089:Glycoside Hydrolase Inhibitors; D000520:alpha-Glucosidases