title: Dual regulation of neuronal morphogenesis by a delta-catenin-cortactin complex and Rho.
abstract: Delta-catenin is a neuronal protein that contains 10 Armadillo motifs and binds to the juxtamembrane segment of classical cadherins. We report that delta-catenin interacts with cortactin in a tyrosine phosphorylation-dependent manner. This interaction occurs within a region of the delta-catenin sequence that is also essential for the neurite elongation effects. Src family kinases can phosphorylate delta-catenin and bind to delta-catenin through its polyproline tract. Under conditions when tyrosine phosphorylation is reduced, delta-catenin binds to cortactin and cells extend unbranched primary processes. Conversely, increasing tyrosine phosphorylation disrupts the delta-catenin-cortactin complex. When RhoA is inhibited, delta-catenin enhances the effects of Rho inhibition on branching. We conclude that delta-catenin contributes to setting a balance between neurite elongation and branching in the elaboration of a complex dendritic tree.
authors.affiliation: Dept. of Neurology, Brigham and Women's Hospital and Harvard Medical School, Harvard Institute of Medicine, 77 Avenue Louis Pasteur, Boston, MA 02115, USA.
authors.forename: Maria Cruz
authors.initials: MC
authors.lastname: Martinez
authors.forename: Tomoyo
authors.initials: T
authors.lastname: Ochiishi
authors.forename: Michael
authors.initials: M
authors.lastname: Majewski
authors.forename: Kenneth S
authors.initials: KS
authors.lastname: Kosik
mesh_terms: D000595:Amino Acid Sequence / Q000502:physiology; D000818:Animals; D051186:Armadillo Domain Proteins; D001921:Brain / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D051177:Catenins; D015815:Cell Adhesion Molecules; D002454:Cell Differentiation / Q000502:physiology*; D048429:Cell Size / Q000502:physiology; D051356:Cortactin; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism*; D003712:Dendrites / Q000378:metabolism / Q000648:ultrastructure; D005333:Fetus; D005822:Genetic Vectors / Q000235:genetics; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D016501:Neurites / Q000378:metabolism* / Q000648:ultrastructure; D016716:PC12 Cells; D010750:Phosphoproteins; D010766:Phosphorylation; D011392:Proline / Q000378:metabolism; D011485:Protein Binding / Q000502:physiology; D017434:Protein Structure, Tertiary / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011993:Recombinant Fusion Proteins; D014443:Tyrosine / Q000378:metabolism; D020742:rhoA GTP-Binding Protein / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D019061:src-Family Kinases / Q000235:genetics / Q000378:metabolism; D000095002:Delta Catenin
chemical_list: D051186:Armadillo Domain Proteins; D051177:Catenins; D015815:Cell Adhesion Molecules; D051356:Cortactin; C495870:Cttn protein, rat; D003598:Cytoskeletal Proteins; D008840:Microfilament Proteins; D010750:Phosphoproteins; D011993:Recombinant Fusion Proteins; D014443:Tyrosine; D011392:Proline; D019061:src-Family Kinases; D020742:rhoA GTP-Binding Protein; D000095002:Delta Catenin