title: The complete genomic sequence of lytic bacteriophage gh-1 infecting Pseudomonas putida--evidence for close relationship to the T7 group.
abstract: The genome of the lytic Pseudomonas putida bacteriophage gh-1 is linear double-stranded DNA containing 37,359 bp with 216-bp direct terminal repeats. Like other members of the T7 group, the gh-1 genome contains regions of high homology to T7 interspersed with nonhomologous regions that contain small open reading frames of unknown function. The genome shares 31 genes in common with other members of the T7 group, including RNA polymerase, and an additional 12 unique putative genes. A major difference between gh-1 and other members of this group is the absence of any open reading frames between the left direct terminal repeat and gene 1. Sequence analysis of the gh-1 genome also revealed the presence of 10 putative phage promoters with a consensus sequence similar to the promoters of T3 and phiYeO3-12 (consensus: TAAAAACCCTCACTRTGGCHSCM). P. putida mutants resistant to gh-1 were demonstrated to have an altered lipopolysaccharide structure, indicating that members of this group use lipopolysaccharide as their cellular receptor.
authors.affiliation: Department of Microbiology and Immunology, Queen's University, Kingston, Ontario K7L 3N6, Canada.
authors.forename: Irina V
authors.initials: IV
authors.lastname: Kovalyova
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Kropinski
mesh_terms: D000595:Amino Acid Sequence; D017123:Bacteriophage T7 / Q000235:genetics*; D001435:Bacteriophages / Q000235:genetics*; D001482:Base Composition; D001483:Base Sequence; D003062:Codon / Q000235:genetics; D004722:Endoribonucleases / Q000378:metabolism; D019143:Evolution, Molecular; D018965:Frameshifting, Ribosomal / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics; D016679:Genome, Viral; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D016958:Pseudomonas putida / Q000235:genetics / Q000821:virology*; D011991:Receptors, Virus / Q000378:metabolism; D043244:Ribonuclease III
chemical_list: D003062:Codon; D011991:Receptors, Virus; D004722:Endoribonucleases; D043244:Ribonuclease III