Simultaneous determination of 16 estrogens, dehydroepiandrosterone and their glucuronide and sulfate conjugates in serum using sodium cholate micelle capillary electrophoresis.
The simultaneous determination of 16 estrogens, dehydroepiandrosterone (DHEA) and their glucuronide and sulfate conjugates by micellar electrokinetic chromatography (MEKC) with sodium cholate micelle is reported. Sodium cholate, sodium dodecylsulfate (SDS) and alpha-, beta-, gamma-cyclodextrins were studied as micelle reagents in the pH range of 7.0-10.0. Estrogens, DHEA and their glucuronide and sulfate conjugates were separated using a 50 cm x 50 microm capillary with 10 mM borate-phosphate buffer (pH 8.0) containing 50 mM sodium cholate as carrier. The method could simultaneously determine 1.0-1000 microg/mL of steroids and metabolites in 100 microL of serum by photometric detection at 214 nm within 14 min and 80 ng/mL steroids could be determined by using 2.0 mL of serum. The relative standards deviations were 6.7-7.7% at 10 microg/mL in serum. The recoveries were 89.1-92.0% with 10 microg/mL serum samples.
Katayama
Masatoki
M
Department of Functional Bioanalysis, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan.
Matsuda
Yoshifumi
Y
Shimokawa
Ken-Ichi
K
Kaneko
Satoru
S
D000328:Adult; D002021:Buffers; D002138:Calibration; D020374:Chromatography, Micellar Electrokinetic Capillary; D003505:Cyclodextrins; D003687:Dehydroepiandrosterone / Q000097:blood*; D019314:Dehydroepiandrosterone Sulfate / Q000097:blood*; D004967:Estrogens / Q000097:blood*; D005260:Female; D005307:Fertilization in Vitro; D020719:Glucuronides / Q000097:blood; D006801:Humans; D007202:Indicators and Reagents; D020358:Sodium Cholate / Q000737:chemistry*; D013431:Sulfates / Q000097:blood
D002021:Buffers; D003505:Cyclodextrins; D004967:Estrogens; D020719:Glucuronides; D007202:Indicators and Reagents; D013431:Sulfates; D003687:Dehydroepiandrosterone; D019314:Dehydroepiandrosterone Sulfate; D020358:Sodium Cholate