title: Crystallization and preliminary X-ray crystallographic analysis of quinolinate phosphoribosyltransferase of Helicobacter pylori.
abstract: Quinolinic acid phosphoribosyltransferase (NadC; EC 2.4.2.19) is the key enzyme of NAD(+) biosynthesis in both prokaryotes and eukaryotes. NadC catalyzes the decarboxylation of quinolinic acid (QA) to produce nicotinic acid mononucleotide (NAMN), an intermediate in NAD synthesis. NadCs of Helicobacter pylori appeared to be a hexamer during the purification procedure. Three different complexes of NadC, with QA, NAMN and phthalic acid (PA), an analogue of QA, were crystallized at 294 +/- 1 K using the hanging-drop vapour-diffusion method. The QA complex crystal was found to belong to space group P4(1)2(1)2, with unit-cell parameters a = b = 148.8, c = 145.7 A, alpha = beta = gamma = 90 degrees. Diffraction data were collected from the NadC-substrate and NadC-substrate analogue complexes to resolutions of 2.3 A (QA), 2.8 A (PA) and 3.3 A (NAMN) using synchrotron X-ray radiation.
authors.affiliation: Department of Life Science, Kwangju Institute of Science and Technology, Gwangju 500-712, South Korea.
authors.forename: Mun-Kyoung
authors.initials: MK
authors.lastname: Kim
authors.forename: Yun Sik
authors.initials: YS
authors.lastname: Kim
authors.forename: Seong-Hwan
authors.initials: SH
authors.lastname: Rho
authors.forename: Young Jun
authors.initials: YJ
authors.lastname: Im
authors.forename: Jun Hyuck
authors.initials: JH
authors.lastname: Lee
authors.forename: Gil Bu
authors.initials: GB
authors.lastname: Kang
authors.forename: Soo Hyun
authors.initials: SH
authors.lastname: Eom
mesh_terms: D003001:Cloning, Molecular; D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray / Q000379:methods; D016480:Helicobacter pylori / Q000201:enzymology*; D009537:Nicotinamide Mononucleotide / Q000031:analogs & derivatives* / Q000737:chemistry; D010430:Pentosyltransferases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D010795:Phthalic Acids / Q000737:chemistry; D011485:Protein Binding; D017378:Quinolinic Acid / Q000737:chemistry; D000098553:Nicotinate-Nucleotide Diphosphorylase (Carboxylating)
chemical_list: D010795:Phthalic Acids; D009537:Nicotinamide Mononucleotide; C002953:nicotinate mononucleotide; C032279:phthalic acid; D010430:Pentosyltransferases; D000098553:Nicotinate-Nucleotide Diphosphorylase (Carboxylating); D017378:Quinolinic Acid