title: Procyanidin B1 is detected in human serum after intake of proanthocyanidin-rich grape seed extract.
abstract: To confirm the absorption of proanthocyanidin (PA) into the human body, four healthy adults were administered 2.0 g of PA-rich grape seed extract (GSE). Blood were drawn before intake and 2 h after intake. Through the enzymatic treatment of sulfatase and beta-glucuronidase, blood samples were analyzed by HPLC coupled with mass-spectrometry (LC/MS). Procyanidin B1 [epicatechin-(4beta-->8)-catechin] was detected in human serum 2 h after intake. Its concentration was 10.6 +/- 2.5 nmol/l.
authors.affiliation: Research and Development Division, Kikkoman Corporation, 399 Noda, Noda, Chiba 278-0037, Japan. asano@mail.kikkoman.co.jp
authors.forename: Atsushi
authors.initials: A
authors.lastname: Sano
authors.forename: Jun
authors.initials: J
authors.lastname: Yamakoshi
authors.forename: Shoichi
authors.initials: S
authors.lastname: Tokutake
authors.forename: Koichiro
authors.initials: K
authors.lastname: Tobe
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Kubota
authors.forename: Mamoru
authors.initials: M
authors.lastname: Kikuchi
mesh_terms: D000975:Antioxidants / Q000493:pharmacokinetics*; D044946:Biflavonoids; D002392:Catechin / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D006801:Humans; D007408:Intestinal Absorption; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000737:chemistry; D044945:Proanthocyanidins / Q000493:pharmacokinetics*; D012639:Seeds / Q000737:chemistry; D027843:Vitis / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D044946:Biflavonoids; D010936:Plant Extracts; D044945:Proanthocyanidins; C479579:procyanidin B1; C013221:proanthocyanidin; C017674:procyanidin; D002392:Catechin