Nonsense mutations in the lsa-like gene in Enterococcus faecalis isolates susceptible to lincosamides and Streptogramins A.
The lsa gene confers intrinsic resistance to lincosamides and streptogramins A in Enterococcus faecalis, probably by active efflux. The lsa-like genes of two clinical isolates of E. faecalis susceptible to lincosamides and dalfopristin contained mutations that produced premature termination codons. Revertant mutants were obtained by selection on agar plates containing clindamycin.
Dina
Julia
J
Service de Microbiologie, CHU Côte de Nacre, 14033 Caen Cedex, France.
Malbruny
Brigitte
B
Leclercq
Roland
R
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018389:Codon, Nonsense; D024881:Drug Resistance, Bacterial / Q000235:genetics; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000235:genetics*; D005798:Genes, Bacterial; D055231:Lincosamides; D018942:Macrolides; D008969:Molecular Sequence Data; D010641:Phenotype; D025364:Streptogramin A / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D018389:Codon, Nonsense; D055231:Lincosamides; D018942:Macrolides; D025364:Streptogramin A