Deletion of a Vibrio cholerae ClC channel results in acid sensitivity and enhanced intestinal colonization.
ClC chloride channels are found in all three kingdoms of life though little is known about their functions in prokaryotes. Here we investigated the role of a Vibrio cholerae ClC channel in acid resistance and intestinal colonization. The putative V. cholerae ClC channel was found to confer mild resistance to acid when pH was adjusted with HCl, but not with other acids. Surprisingly, a ClC channel deletion mutant exhibited enhanced intestinal colonization in infant mice.
Ding
Yanpeng
Y
Division of Geographic Medicine and Infectious Diseases, Tufts-New England Medical Center and Howard Hughes Medical Institute, Boston, Massachusetts 02111, USA.
Waldor
Matthew K
MK
D000143:Acids / Q000494:pharmacology; D000818:Animals; D000833:Animals, Suckling; D018118:Chloride Channels / Q000502:physiology*; D002712:Chlorides / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000382:microbiology*; D051379:Mice; D014734:Vibrio cholerae / Q000502:physiology*
D000143:Acids; D018118:Chloride Channels; D002712:Chlorides