title: Genomic structure, conservation and FISH mapping of the Rattus norvegicus Adprt gene.
abstract: Poly(ADP-ribose) polymerase 1 (PARP-1) lies at the basis of a DNA-interacting protein family that maintains genome integrity. Here we describe the genomic organisation of rat PARP-1 gene (Adprt), refine its assignment to rat chromosome (RNO) 13q25-->q26 by FISH and compare its genomic organisation between rat, mouse and human. It appears that in human, mouse and rat Adprt consists of 23 similar-sized exons with well-conserved intron and exon borders. Adprt orthologs map to homologous chromosome regions at the termini of the q-arms of human and mouse chromosomes 1 and rat 13, with gene order being conserved between the rodents. Kimura protein distance comparison with human PARP-1 as reference revealed the bovine protein to be the least conserved with 10.3 substitutions per 100 amino acids, followed by rat (8.6) and mouse (8.4).
authors.affiliation: Abteilung Tumorvirologie, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, Heidelberg, Germany. benekeus@hotmail.com
authors.forename: S
authors.initials: S
authors.lastname: Beneke
authors.forename: R
authors.initials: R
authors.lastname: Kappler
authors.forename: A
authors.initials: A
authors.lastname: Bürkle
authors.forename: H
authors.initials: H
authors.lastname: Scherthan
mesh_terms: D036002:ADP Ribose Transferases / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002460:Cell Line; D002874:Chromosome Mapping; D017124:Conserved Sequence; D005091:Exons; D016678:Genome; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D010802:Phylogeny; D051381:Rats
chemical_list: D036002:ADP Ribose Transferases