Cytotoxic necrotizing factor 1 enhances reactive oxygen species-dependent transcription and secretion of proinflammatory cytokines in human uroepithelial cells.
Uropathogenic Escherichia coli strains frequently produce a Rho-activating protein toxin named cytotoxic necrotizing factor type 1 (CNF1). We herein report that CNF1 promotes transcription and release of tumor necrosis factor alpha, gamma interferon, interleukin-6 (IL-6), and IL-8 proinflammatory cytokines and increases the production of reactive oxygen species (ROS) in uroepithelial T24 cells. The antioxidant N-acetyl-L-cysteine counteracts these phenomena, a fact which suggests a role for ROS-mediated signaling in CNF1-induced proinflammatory cytokine production.
Falzano
Loredana
L
Department of Ultrastructures, Istituto Superiore di Sanità, 00161 Rome, Italy.
Quaranta
Maria Giovanna
MG
Travaglione
Sara
S
Filippini
Perla
P
Fabbri
Alessia
A
Viora
Marina
M
Donelli
Gianfranco
G
Fiorentini
Carla
C
D001427:Bacterial Toxins / Q000633:toxicity*; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis*; D003603:Cytotoxins / Q000633:toxicity*; D029968:Escherichia coli Proteins; D006801:Humans; D017382:Reactive Oxygen Species; D014158:Transcription, Genetic / Q000187:drug effects*; D001743:Urinary Bladder / Q000187:drug effects* / Q000378:metabolism
D001427:Bacterial Toxins; D016207:Cytokines; D003603:Cytotoxins; D029968:Escherichia coli Proteins; D017382:Reactive Oxygen Species; C060296:cytotoxic necrotizing factor type 1