title: Inflammatory mediators in autotransfusion drain blood after knee arthroplasty, with and without leucocyte reduction.
abstract: OBJECTIVE
  The aim of this study was to evaluate whether leucocyte-reducing filters influenced complement activation and the formation of pro-inflammatory cytokines in autotransfusion drain blood after knee arthroplasty.
  
  
  METHODS
  Twenty-three patients undergoing knee arthroplasty were divided into two groups. All patients were given salvage blood postoperatively. In Group A, a leucocyte filter was connected between the wound and the drain blood container. In Group B the drain blood was not leucocyte filtered. Complement split products and cytokines were analysed in circulating blood and in drain blood, together with blood-cellular differential counts.
  
  
  RESULTS
  Drain blood showed activation vs. venous blood, with elevated concentrations of C3a, SC5b-9, interleukin (IL)-6, IL-8, polymorphonuclear (PMN) elastase and tumour necrosis factor-alpha (TNF-alpha) (P<0.05 to P<0.001). The leucocyte filter reduced TNF-alpha (P<0.01), but triggered complement activation (P<0.05). Room-temperature incubation increased the concentration of IL-8 (P<0.01), which was seen in both venous and drain blood. The leucocyte filter prevented formation of IL-8 (P<0.01). In drain blood at 24 h the inflammatory reactions accelerated (P<0.05-0.001), although the filter reduced the leucocyte counts and TNF-alpha concentrations.
  
  
  CONCLUSIONS
  The leucocyte filter reduced IL-8 and TNF-alpha in drain blood, but at the same time triggered complement activation. Incubation affected the inflammatory spectrum of both drain blood and control venous blood, and the filtering reduced this activation.
authors.affiliation: Department of Surgical and Perioperative Science, Umeå University Hospital, Sweden.
authors.forename: T
authors.initials: T
authors.lastname: Dalén
authors.forename: A
authors.initials: A
authors.lastname: Bengtsson
authors.forename: B
authors.initials: B
authors.lastname: Brorsson
authors.forename: K G
authors.initials: KG
authors.lastname: Engström
mesh_terms: D000368:Aged; D019645:Arthroplasty, Replacement, Knee; D001804:Blood Transfusion, Autologous / Q000379:methods*; D002469:Cell Separation; D003167:Complement Activation; D016207:Cytokines / Q000097:blood; D004322:Drainage; D005260:Female; D005374:Filtration; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood*; D016209:Interleukin-8 / Q000097:blood; D007958:Leukocyte Count; D007962:Leukocytes; D008297:Male; D011182:Postoperative Care; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha