title: Proliferation, migration, and invasion of human glioma cells exposed to fractionated radiotherapy in vitro.
abstract: Radiotherapy is a well established treatment for malignant gliomas. This study describes the migration, proliferation, and invasion behaviour of two human glioma cell lines (GaMg and U-87 Mg) grown as multicellular tumour spheroids after radiotherapy. Migration and proliferation studies were performed using conventional and accelerated fractionation up to 60 Gy and 59.4 Gy, respectively. A dose-dependent growth and migratory response to irradiation independent of the type of fractionation was observed. A coculture system in which tumour spheroids were confronted with foetal rat brain aggregates was used for invasion studies. Marked invasion of the glioma spheroids into the brain aggregates occurred with or without radiotherapy. For the GaMg cells, flow cytometric DNA histograms after treatment with 10 Gy and 40 Gy showed an accumulation of cells in the G2/M phase of the cell cycle. Radiotherapy inhibits tumour cell growth and migration, but the invasiveness of the remaining tumour cells seems to be unaffected.
authors.affiliation: Department of Neurosurgery, Medical University of Lübeck, Ratzeburger Allee 160, 23538, Lübeck, Germany. jan.gliemroth@t-online.de
authors.forename: Jan
authors.initials: J
authors.lastname: Gliemroth
authors.forename: Thomas
authors.initials: T
authors.lastname: Feyerabend
authors.forename: Christiane
authors.initials: C
authors.lastname: Gerlach
authors.forename: Hans
authors.initials: H
authors.lastname: Arnold
authors.forename: A Jorge A
authors.initials: AJ
authors.lastname: Terzis
mesh_terms: D000818:Animals; D002455:Cell Division / Q000502:physiology / Q000528:radiation effects*; D002465:Cell Movement / Q000502:physiology / Q000528:radiation effects*; D002470:Cell Survival / Q000502:physiology / Q000528:radiation effects*; D019583:Dose Fractionation, Radiation; D005434:Flow Cytometry; D005910:Glioma / Q000503:physiopathology / Q000532:radiotherapy*; D006801:Humans; D066298:In Vitro Techniques; D009361:Neoplasm Invasiveness / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar; D018874:Spheroids, Cellular / Q000502:physiology / Q000528:radiation effects / Q000648:ultrastructure; D014407:Tumor Cells, Cultured / Q000502:physiology / Q000528:radiation effects / Q000648:ultrastructure