M-phase regulation of the recruitment of mRNAs onto polysomes using the CDK1/cyclin B inhibitor aminopurvalanol.
Translation under the control of the universal cell cycle regulator CDK1/cyclin B was investigated during the first cell cycle in sea urchin embryos. The CDK1/cyclin B inhibitor aminopurvalanol arrested embryos at the G2/M transition. Polysomal mRNAs were purified from control and arrested embryos, and screened for specific mRNA recruitment or release at M-phase by subtractive hybridization. The polysomal repartition of clones issued from this screen was analyzed. Three specific mRNAs were selectively recruited onto polysomes at M-phase. Conversely, two other specific mRNAs were released from polysomes. The isolation of these translationally regulated mRNAs gives now important tools for insights into the regulation of protein synthesis by the cell cycle regulator CDK1-cyclin B.
Le Breton
Magali
M
Station Biologique de Roscoff, UMR 7127, CNRS/UPMC, BP 74, 29682, Roscoff Cedex, France.
Bellé
Robert
R
Cormier
Patrick
P
Mulner-Lorillon
Odile
O
Morales
Julia
J
D000818:Animals; D016203:CDC2 Protein Kinase / Q000037:antagonists & inhibitors*; D019926:Cyclin B / Q000037:antagonists & inhibitors*; D004305:Dose-Response Relationship, Drug; D015180:Electrophoresis, Gel, Two-Dimensional; D005306:Fertilization; D016195:G2 Phase; D008938:Mitosis; D009693:Nucleic Acid Hybridization; D011132:Polyribosomes / Q000378:metabolism*; D014176:Protein Biosynthesis; D011687:Purines / Q000494:pharmacology*; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012617:Sea Urchins; D013997:Time Factors
D019926:Cyclin B; D011687:Purines; D012333:RNA, Messenger; D012313:RNA; D016203:CDC2 Protein Kinase