[FCM detection and pathology observation of 5 mm cutting-edge of laryngocarcinoma].
OBJECTIVE
To explore the safety of 5 mm cutting-edges with the analysis of DNA contend and cell cycle and the pathology observation.


METHODS
Twenty specimens of laryngocarcinoma and its cutting-edges were analyzed by flow cytometry, contrasting to 5 samples of normal larynx tissue, and their light-microscopy and electra-microscopy were discussed.


RESULTS
The DNA aneuploidy(AN) rate in the laryngocarcinoma, its cutting-edges and the normal tissue is respectively 60% (12/20), 15%(3/20) and 0. The rate of S phrase of the laryngocarcinoma and its cutting-edges is respectively 20.33 +/- 6.0 and 12.35 +/- 3.81, so the DI 1.78 +/- 0.82 and 1.07 +/- 0.04, PI 29.09 +/- 6.8 and 11.75 +/- 3.75, there are significant differences (P < 0.05). The light-microscopy showed 5 mm cutting-edges were infiltrated by carcinoma in the form of submucosa or extention of carcinoma in situ and the results was, to some extension, consistent with the findings of FCM.


CONCLUSIONS
The safety of 5 mm cutting-edge varies depending on whether invading paraglottic space.
Chen
Xiaohong
X
Department of Otorhinolaryngology, Anhui Province Hospital, Hefei 230001.
Sun
Jingwu
J
Hu
Yanming
Y
Ye
Feichang
F
Wang
Mingshan
M
Jin
Zicang
Z
Huang
Zhigang
Z
Han
Demin
D
D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology* / Q000601:surgery; D002453:Cell Cycle; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics / Q000473:pathology* / Q000601:surgery; D008297:Male; D008875:Middle Aged
D004273:DNA, Neoplasm