title: IMGT/PhyloGene: an on-line tool for comparative analysis of immunoglobulin and T cell receptor genes.
abstract: IMGT/PhyloGene is an on-line software package for comparative analysis of immunoglobulin (IG) and T cell receptor (TR) variable genes of all vertebrate species, newly implemented in IMGT, the international ImMunoGeneTics information system ((R)). IMGT/PhyloGene is strongly associated with the IMGT gene and allele nomenclature and with the IMGT unique numbering for V-REGION, which directly creates standardized alignments from IMGT reference sequences. IMGT/PhyloGene is the first tool to use the IMGT expertized and standardized data for automated comparative analyses, and the first on-line software package for phylogenetic reconstruction to be integrated to a sequence database. Starting from a standardized alignment of selected sequences, IMGT/PhyloGene computes a matrix of evolutionary distances, builds a tree using the Neighbor-Joining (NJ) algorithm, and outputs various graphical tree representations. The resulting IMGT/PhyloGene tree is then used as a support for studying the evolution of particular subregions, such as the CDR-IMGT (Complementarity Determining Regions) or the V-RS (Variable gene Recombination Signals). IMGT/PhyloGene is freely available at http://imgt.cines.fr.
authors.affiliation: IMGT, the International ImMunoGeneTics Information System, Laboratoire d'ImmunoGénétique Moléculaire (LIGM), Université Montpellier II, UPR CNRS 1142, Institut de Génétique Humaine (IGH), 141 rue de la Cardonille, 34396 Cedex 5, Montpellier, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Elemento
authors.forename: Marie Paule
authors.initials: MP
authors.lastname: Lefranc
mesh_terms: D030561:Databases, Nucleic Acid; D007136:Immunoglobulins / Q000235:genetics*; D009862:Online Systems; D010802:Phylogeny; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D007136:Immunoglobulins; D011948:Receptors, Antigen, T-Cell