title: Photoreactivity of 5-iodouracil-containing DNA-Z alpha complex.
abstract: It is revealed that double-strand RNA adenosine deaminase 1 binds to Z-DNA and NH2-terminus Z alpha domain is responsible for the high affinity binding. In order to investigate the structure of Z-DNA induced by Z alpha, the photoreactions of 5-halouracil-containing Z-DNA in the presence and the absence of Z alpha were investigated. It was demonstrated that the C2' alpha-hydroxylated product was efficiently produced in Z-DNA induced by Z alpha compared to that under high salt concentration.
authors.affiliation: Division of Biofunctional Molecules, Institute of Biomaterials and Bioengineering, Tokyo Med. & Den. University, Tokyo 101-0062, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Oyoshi
authors.forename: H
authors.initials: H
authors.lastname: Sugiyama
mesh_terms: D000243:Adenosine Deaminase / Q000737:chemistry / Q000378:metabolism*; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism / Q000528:radiation effects*; D008027:Light; D046911:Macromolecular Substances; D009690:Nucleic Acid Conformation; D017434:Protein Structure, Tertiary; D016601:RNA-Binding Proteins; D014498:Uracil / Q000031:analogs & derivatives* / Q000737:chemistry*
chemical_list: D046911:Macromolecular Substances; D016601:RNA-Binding Proteins; D014498:Uracil; D004247:DNA; C567955:ADARB1 protein, human; D000243:Adenosine Deaminase; C048747:5-iodouracil