[Study of RB gene in carcinoma of larynx].
OBJECTIVE
To probe the mutations of RB gene in laryngeal carcinoma, and the relation between it and the clinical phase.


METHODS
The mutations were detected by PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism).


RESULTS
In laryngeal carcinoma, the mutations were identified in 14(15.5%) of 90 samples, of which 2 were large deletion, 12 were abnormal in electrophoresis of SSCP. The mutations of I-II stage tumor were 5(13.9%), of III-IV were 9(16.7%), there wasn't a significant difference between them.


CONCLUSIONS
RB gene mutation takes part in the development of laryngeal carcinoma. But there wasn't relation between RB gene mutation and the course of laryngeal carcinoma.
Wang
Binquan
B
Department of Otorhinolaryngology, First Clinical Hospital, Shanxi Medical University, Taiyuan 030001.
Chen
Yanqiu
Y
Wen
Shuxin
S
Wang
Jianming
J
Huangpu
Hui
H
Li
Yujun
Y
D016161:Genes, Retinoblastoma / Q000235:genetics*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000473:pathology; D008875:Middle Aged; D009154:Mutation; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational