Fabin, a novel calcyon-like and glucanase-like protein with mitogenic, antifungal and translation-inhibitory activities from broad beans.
A protein with an N-terminal sequence displaying similarities to N-terminal sequences of human calcyon and barley endo-1,4-glucanase, and to C-terminal sequences of human translation initiation factor 4 gamma and yeast superkiller viralicidic activity, was isolated from the broad bean Vicia faba. The protein, termed fabin, has a molecular mass of 34 kDa in SDS-polyacrylamide gel electrophoresis. Antifungal activity of the protein was observed against several fungal species including Rhizoctonia solani, Botrytis cinerea, Fusarium oxysporum and Mycosphaerella arachidicola. Fabin inhibits HIV-1 reverse transcriptase with an IC50 of 34 microM and translation in a rabbit reticulocyte lysate with an IC50 of 2.4 microM. At a concentration of about 1.5 microM fabin is able to elicit a 9-fold increase in the mitogenic response of murine splenocytes.
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Department of Biochemistry, Faculty of Medicine, Chinese University of Hong Kong, Shatin, Hong Kong, China.
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D000595:Amino Acid Sequence; D000818:Animals; D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002480:Cellulase / Q000737:chemistry* / Q000235:genetics / Q000494:pharmacology; D005658:Fungi / Q000187:drug effects; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000494:pharmacology; D051379:Mice; D008934:Mitogens / Q000302:isolation & purification / Q000494:pharmacology; D008970:Molecular Weight; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects; D013154:Spleen / Q000166:cytology; D031307:Vicia faba / Q000737:chemistry*
D000935:Antifungal Agents; D008565:Membrane Proteins; D008934:Mitogens; D010940:Plant Proteins; C405464:calcyon; D002480:Cellulase