[Effects of rotating clinostat simulated weightlessness on the differentiation-related gene expression of ROS17/2.8 cells].
OBJECTIVE
To investigate the effects of rotating clinostat simulated weightlessness on the differentiation-related gene expression of ROS17/2.8 (rat osteosarcoma) cells.


METHODS
Cultured ROS17/2.8 cells were exposed to rotating clinostat simulated weightlessness for 24, 48 and 72 h with control group in 1 G condition. Total RNA in cells was isolated and reverse transcription PCR analysis was made. Gene expression of type I collagen alpha 1 chain and alkaline phosphatase were examined by their ratios to beta-actin.


RESULTS
The expression of type I collagen alpha I chain and alkaline phosphatase (ALP) gene was significantly lower after 72 h exposure to clinostat simulated weightlessness than that of the control group (P<0.01).


CONCLUSIONS
Differentiation of rat osteosarcoma cells was reduced after exposure to rotating clinostat simulated weightlessness for 72 h.
Wang
Bing
B
Faculty of Aerospace Medicine, The Fourth Military Medical University, Xi'an, China.
Zhang
Shu
S
Wu
Xing-yu
XY
D000469:Alkaline Phosphatase / Q000235:genetics*; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics*; D015870:Gene Expression; D012516:Osteosarcoma / Q000235:genetics / Q000473:pathology*; D051381:Rats; D012399:Rotation; D013997:Time Factors; D018474:Weightlessness Simulation
D024042:Collagen Type I; D000469:Alkaline Phosphatase