Expression of claudin-5 in dermal vascular endothelia.
Claudins and occludin are integral membrane proteins at tight junctions (TJs). We examined subcellular localization of claudin-5 and occludin in dermal vascular endothelia. Immunofluorescence staining showed that claudin-5 was expressed at the cell-cell border of dermal vascular endothelia in mouse skin. However, in some dermal vessels, claudin-5 expression was markedly decreased or absent in amount by double-immunofluorescence stainings with PECAM-1 and PAL-E. In contrast, occludin was not detected in dermal vessels. Freeze-fracture and immunoreplica electron microscopy on primary-cultured human dermal endothelial cells showed that claudin-5 was localized at tight junctions. These findings confirmed that TJs in dermal vascular endothelial cells are composed of claudin-5.
Morita
Kazumasa
K
Department of Dermatology, Graduate School of Medicine, Kyoto University, Kyoto, Japan. kamorita@kuhp.kyoto-u.ac.jp
Sasaki
Hiroyuki
H
Furuse
Kyoko
K
Furuse
Mikio
M
Tsukita
Shoichiro
S
Miyachi
Yoshiki
Y
D000818:Animals; D002478:Cells, Cultured; D062507:Claudin-5; D020405:Dermis / Q000098:blood supply*; D004730:Endothelium, Vascular / Q000502:physiology* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D005614:Freeze Fracturing; D015870:Gene Expression; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D062793:Occludin; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism
D062507:Claudin-5; C491511:Cldn5 protein, mouse; D008565:Membrane Proteins; C568985:OCLN protein, human; D062793:Occludin; C568986:Ocln protein, mouse; D019408:Platelet Endothelial Cell Adhesion Molecule-1