[A composite polyaniline-containing silica sorbent for DNA isolation].
A composite sorbent based on porous glass beads modified with thin polyaniline coating was prepared by precipitating aniline polymerization in the presence of carrier particles. It was shown that the modification ensures the uniform coating of the inner surface of the carrier pores with the polymer layer approximately 70 A thick. It was shown that the resulting material retains the initial porosity of the carrier and is selective in the separation of nucleic acids and proteins. The polyaniline-coated sorbents were shown to be efficient for both the preparative DNA isolation from bacterial lysates and for analytical purposes, in particular, for studying DNA fragmentation during apoptosis proceeding under UV irradiation of cell lysates of colon carcinoma. The morphological and chromatographic characteristics of the new sorbent were demonstrated to be similar to those of the polyfluorobutadiene sorbent.
Kapustin
D V
DV
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, GSP Moscow, 117997 Russia. kapustin@mail.ibch.ru
Iagudaeva
E Iu
EIu
Zavada
L L
LL
Zhigis
L S
LS
Zubov
V P
VP
Iaroshevskaia
E M
EM
Plobner
L
L
Leiser
R M
RM
Brem
G
G
D000814:Aniline Compounds / Q000737:chemistry*; D017209:Apoptosis / Q000235:genetics / Q000528:radiation effects; D001671:Biochemistry / Q000379:methods*; D002277:Carcinoma / Q000235:genetics / Q000473:pathology; D002845:Chromatography / Q000379:methods; D003110:Colonic Neoplasms / Q000235:genetics / Q000473:pathology; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000302:isolation & purification; D004273:DNA, Neoplasm / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D012822:Silicon Dioxide / Q000737:chemistry*; D014466:Ultraviolet Rays
D000814:Aniline Compounds; D004269:DNA, Bacterial; D004273:DNA, Neoplasm; C416807:polyaniline; D012822:Silicon Dioxide; D004247:DNA