Solution structures of the DNA complexes with alkyl-linked bis(benzimidazole) compounds studied by NMR spectroscopy.
We have synthesized two novel alkyl-linked bis(benzimidazole) compounds related to pentamidine. The solution structures of 1:1 complexes of these compounds with the self-complementary DNA duplexes, d(CGCGAATTCGCG)2 (A2T2) and d(CGCAAATTTGCG)2 (A3T3) have been studied by one- and two-dimensional 1H NMR spectroscopy. The intermolecular NOE data of the A3T3 complexed with compound 3 indicate that 3 binds in the minor groove of the central 5'-CAAATTTG region of A3T3.
Kubota
Y
Y
Department of Chemistry, Faculty of Science, Yamaguchi University, Yamaguchi 753-8512, Japan.
Tani
S
S
Fujii
H
H
D000478:Alkylation; D001483:Base Sequence; D001562:Benzimidazoles / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D046911:Macromolecular Substances; D019906:Nuclear Magnetic Resonance, Biomolecular
D001562:Benzimidazoles; D046911:Macromolecular Substances; C009712:bis-benzimidazole; D004247:DNA