Transcriptional regulation of interferon gamma gene by p300 co-activator.
Interferon gamma (IFN gamma) is an important immunoreguratory protein and is secreted from CD4+, CD8+ T cells and NK cells. Expression of IFN gamma gene is regulated by either DNA-methylation or cis-elements of transcriptional factors such as AP-1, NFAT, AP-4, YY-1, ATF and GATA. At a putative ATF binding site in a promoter region of IFN gamma gene, the position from -59 to -43, is important for the IFN gamma promoter activity. Our gel-shift competition studies demonstrated that addition of antibodies against ATF-2 caused super-shifts of DNA-protein complexes. In addition, it was clarified that p300 was involved in DNA-protein complexes by in vitro "pull-down" experiments. Moreover, a co-expression of p300 and ATF-2 enhanced the promoter activity of IFN gamma gene. Thus, these results indicate that p300 and ATF-2 play critical rules in a basal transcription of IFN gamma gene in Jurkat T cells.
Nakayama
A
A
Institute of Applied Biochemistry, University of Tsukuba, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 4, Tsukuba, Japan.
Kawasaki
H
H
Jin
C
C
Munekata
E
E
Taira
K
K
Yokoyama
K K
KK
D051698:Activating Transcription Factor 2; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D017930:Genes, Reporter; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D019169:Jurkat Cells; D008156:Luciferases / Q000032:analysis / Q000235:genetics; D009687:Nuclear Proteins / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D013601:T-Lymphocytes / Q000276:immunology; D015534:Trans-Activators / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism; D015533:Transcriptional Activation
C497105:ATF2 protein, human; D051698:Activating Transcription Factor 2; D017362:Cyclic AMP Response Element-Binding Protein; D009687:Nuclear Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D007371:Interferon-gamma; D008156:Luciferases