Synthesis of a fluorescent chemosensor suitable for the imaging of zinc(II) in live cells.
The synthesis of a coumarin-cyclen conjugate-based zinc-specific chemosensor and its ability to sense Zn(2+) in vitro is described. Using fluorescence microscopy, the chemosensor was shown to be capable of imaging Zn(2+) in live rat pituitary tumour cells.
Lim
Nathaniel C
NC
University of Connecticut, Department of Chemistry, 06269-3060, Storrs, CT, USA.
Yao
Lili
L
Freake
Hedley C
HC
Brückner
Christian
C
D000818:Animals; D045744:Cell Line, Tumor; D002614:Chelating Agents / Q000138:chemical synthesis; D003374:Coumarins / Q000138:chemical synthesis; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D007202:Indicators and Reagents; D007700:Kinetics; D008856:Microscopy, Fluorescence; D010911:Pituitary Neoplasms / Q000378:metabolism / Q000473:pathology; D051381:Rats; D015032:Zinc / Q000378:metabolism*
D002614:Chelating Agents; D003374:Coumarins; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D015032:Zinc