A model system of primary murine hepatocytes infected by murine cytomegalovirus.
In order to establish a model system of the murine hepatocyte infection by murine cytomegalovirus (MCMV), the primary cultured murine hepatocytes were obtained in a modified low-serum medium system by a non-perfusion method, and then infected by Smith strain MCMV. Infected hepatocytes showed characteristic cytopathic effect (CPE) at 30 h after infection, in which a large number of viral particles was found and ultrastructures were destroyed (as revealed by disappearance of bile canalicula and organelles) under the electron microscope and MCMV immediate-early genes were detected by in situ hybridization. Meanwhile, infected cells produced albumin significantly less than corresponding uninfected controls. On the contrary, uninfected controls simultaneously cultured under the same conditions showed normal function and ultrastructure (glycogen rosettes, bile canalicula, wheel-like mitochondria and well-developed rough and smooth endoplasmic reticula). These results demonstrated that a model system of primary cultured murine hepatocytes infected by MCMV was successfully set up.
Fang
F
F
Department of Pediatrics, Tongji Hospital, Tongji Medical University, Wuhan 430030.
Nie
X
X
Li
G
G
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D005260:Female; D022781:Hepatocytes / Q000648:ultrastructure / Q000821:virology*; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018146:Muromegalovirus / Q000254:growth & development / Q000472:pathogenicity*; D014779:Virus Replication