Characterization of salicylic acid-induced genes in Chinese cabbage.
Salicylic acid is a messenger molecule in the activation of defense responses in plants. In this study, we isolated four cDNA clones representing salicylic acid-induced genes in Chinese cabbage (Brassica rapa subsp. pekinensis) by subtractive hybridization. Of the four clones, the BC5-2 clone encodes a putative glucosyltransferase protein. The BC5-3 clone is highly similar to an Arabidopsis gene encoding a putative metal-binding farnesylated protein. The BC6-1 clone is a chitinase gene with similarities to a rapeseed class IV chitinase. Class IV chitinases have deletions in the chitin-binding and catalytic domains and the BC6-1 chitinase has an additional deletion in the catalytic domain. The BCP8-1 clone is most homologous to an Arabidopsis gene that contains a tandem array of two thiJ-like sequences. These four cabbage genes were barely expressed in healthy leaves, but were strongly induced by salicylic acid and benzothiadiazole. Expression of the three genes represented by the BC5-2, BC5-3 and BCP8-1 clones were also induced by Pseudomonas syringae pv. tomato, a nonhost pathogen that elicits a hypersensitive response in Chinese cabbage. None of these four genes, however, was strongly induced by methyl jasmonate or by ethylene.
Park
Y-S
YS
Division of Life Sciences, Chungbuk National University, 360-763 Cheongju, Korea.
Min
H-J
HJ
Ryang
S-H
SH
Oh
K-J
KJ
Cha
J-S
JS
Kim
H Y
HY
Cho
T-J
TJ
D000085:Acetates / Q000494:pharmacology; D000595:Amino Acid Sequence; D029741:Brassica rapa / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics* / Q000382:microbiology; D002688:Chitinases / Q000737:chemistry / Q000235:genetics; D003517:Cyclopentanes / Q000494:pharmacology; D005030:Ethylenes / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017343:Genes, Plant / Q000235:genetics*; D016695:Glycosyltransferases / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D054883:Oxylipins; D011549:Pseudomonas / Q000502:physiology; D020156:Salicylic Acid / Q000494:pharmacology*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013830:Thiadiazoles / Q000494:pharmacology
D000085:Acetates; D003517:Cyclopentanes; D005030:Ethylenes; D054883:Oxylipins; D013830:Thiadiazoles; C015700:benzo-1,2,3-thiadiazole; C072239:methyl jasmonate; C036216:ethylene; D016695:Glycosyltransferases; D002688:Chitinases; D020156:Salicylic Acid