Purification and characterization of laminaran hydrolases from Trichoderma viride.
At least three extracellular laminaran hydrolases which hydrolyzed laminaran (beta-1,3:1,6-glucan) from Eisenia bicyclis were secreted in wheat bran solid medium by Trichoderma viride U-1. These three enzymes, lam AI, AII, and B, were purified to electrophoretic homogeneity. Their molecular masses were estimated to be 70.1, 70.4, and 45.0 kDa for lam AI, AII, and B, respectively, by SDS-PAGE. Whereas both lam AI and AII could hydrolyze laminarin from Laminaria digitata, lam AII showed higher activity against Laminaria laminarin rather than Eisenia laminaran. On the other hand, lam B preferentially hydrolyzed pustulan, a beta-1,6-glucan. Laminarioligosaccharide was hydrolyzed by lam AI and AII but not B, whereas gentiooligosaccharide was hydrolyzed by only lam B. It showed that lam AI and AII were specific for beta-1,3-linkages, but lam B was specific for beta-1,6-linkages. These results indicated that T. viride U-1 has a multiple glucanolytic enzyme system.
Nobe
Rika
R
Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, Miyazaki University, 1-1 Gakuen Kibanadai Nishi, Miyazaki 889-2192, Japan.
Sakakibara
Yoichi
Y
Fukuda
Nobuhiro
N
Yoshida
Naoto
N
Ogawa
Kihachiro
K
Suiko
Masahito
M
D005656:Fungal Proteins / Q000302:isolation & purification; D043326:Glucan 1,3-beta-Glucosidase / Q000302:isolation & purification / Q000378:metabolism; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000302:isolation & purification / Q000378:metabolism; D005936:Glucans; D006026:Glycoside Hydrolases / Q000302:isolation & purification* / Q000378:metabolism*; D008970:Molecular Weight; D009097:Multienzyme Complexes; D011134:Polysaccharides / Q000378:metabolism*; D013379:Substrate Specificity; D014242:Trichoderma / Q000201:enzymology*
D005656:Fungal Proteins; D005936:Glucans; D009097:Multienzyme Complexes; D011134:Polysaccharides; C008247:laminaran; D006026:Glycoside Hydrolases; D004693:Glucan Endo-1,3-beta-D-Glucosidase; D043326:Glucan 1,3-beta-Glucosidase