title: Apnoeic responses to intracarotid nicotine challenge in anaesthetized cats.
abstract: To determine the effects of an intraarterial administration of nicotine on the occurrence of apnoea and the activity of rib cage respiratory muscles, we studied 31 anaesthetized, spontaneously breathing cats. Phrenic activity was used as an index of neural inspiratory drive. Activity of parasternal intercostal (PIM) and triangularis sterni (TS) muscles was recorded. Nicotine in a dose of 65 microg/kg was injected into the left common carotid artery prior to and after midcervical vagotomy, preceded by section of the superior laryngeal nerves (SLNs). In eight additional cats, initially neurotomized as mentioned, nicotine was injected after bilateral disruption of the carotid sinus nerves (CSNs). Nicotine induced prompt expiratory apnoea of mean duration of 5.4+/-0.3s in 19 non-vagotomized and of 5.92+/-0.51 s (mean+/-S.E.M.) in 13 vagotomized cats. The occurrence and duration of the temporary arrest of breathing were reduced by midcervical vagotomy but not by subsequent CSNs neurotomy, which abolished post-apnoeic acceleration of breathing. In post-nicotine breathing of increased tidal volume and respiratory rate, peak activity of the parasternal intercostal muscles increased from baseline of 3.2+/-1.2 to 9.5+/-2.0 arbitrary units (p<0.001). The peak height of the phrenic nerve elevated from 7.9+/-0.9 to 14.5+/-1.7 arbitrary units (p<0.001). That of the triangularis sterni showed no change. The response of the respiratory effectors elicited by nicotine was independent of the vagal integrity and may be attributed to activation of nicotine receptors within the brainstem respiratory neurones.
authors.affiliation: Laboratory of Respiration Physiology, Department of Neurophysiology, PAS Medical Research Centre, Warsaw, Poland. szereda@cmdik.pan.pl
authors.forename: M
authors.initials: M
authors.lastname: Szereda-Przestaszewska
authors.forename: B
authors.initials: B
authors.lastname: Kopczyńska
mesh_terms: D000758:Anesthesia / Q000379:methods*; D000818:Animals; D001049:Apnea / Q000139:chemically induced* / Q000503:physiopathology; D017536:Carotid Artery, Common / Q000187:drug effects* / Q000502:physiology; D002415:Cats; D005260:Female; D007269:Injections, Intra-Arterial; D008297:Male; D009538:Nicotine / Q000008:administration & dosage*; D010791:Phrenic Nerve / Q000187:drug effects / Q000502:physiology; D012119:Respiration / Q000187:drug effects*; D014628:Vagotomy / Q000379:methods
chemical_list: D009538:Nicotine