Induction of differentiation in human promyelocytic leukemia HL-60 cell line by niacin-related compounds.
Water-soluble vitamin, niacin, and its related compounds were examined for their differentiation-inducing activity in human promyelocytic leukemia cells (HL-60). Among the compounds, which inhibited cell proliferation measured by MTT assay, isonicotinic acid, nicotinamide N-oxide, and nicotinamide induced NBT reducing activity. HL-60 cells were differentiated into granulocyte-like cells by these compounds, judging from morphological changes and loss of nonspecific esterase activity. The differentiation-inducing activity of water-soluble vitamin and its related compounds suggest that these compounds may be applicable for medical use.
Iwata
Keiko
K
Laboratory of Molecular and Cellular Biology, Department of Life Science, Faculty of Bioresources, Mie University, Tsu, Mie 514-8507, Japan.
Ogata
Shin
S
Okumura
Katsuzumi
K
Taguchi
Hiroshi
H
D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D018922:HL-60 Cells; D006801:Humans; D007539:Isonicotinic Acids / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009525:Niacin / Q000031:analogs & derivatives* / Q000494:pharmacology*
D007539:Isonicotinic Acids; D009525:Niacin; D007770:L-Lactate Dehydrogenase