title: The 1.6 A resolution structure of Fe-superoxide dismutase from the thermophilic cyanobacterium Thermosynechococcus elongatus.
abstract: The iron-containing superoxide dismutase (FeSOD) from the thermophilic cyanobacterium Thermosynechococcus elongatus has been isolated. The protein crystallizes readily and we have determined the structure to 1.6 A resolution. This is the first structural characterization of an FeSOD isolated from a cyanobacterium and one of the highest resolution FeSOD structures determined to date. The activity of the T. elongatus FeSOD has been measured both at 25 degrees C and 50 degrees C and it has been spectroscopically characterized. The T. elongatus FeSOD EPR spectra at pH 5.1, 7.5 and 10.0 are similar. This indicates that no change in the geometry of the Fe(III) site occurs over a wide range of pH. This is in contrast to the other FeSODs described in the literature.
authors.affiliation: Molecular Biology Institute, UCLA, Box 951570, Los Angeles, CA 90095-1570, USA.
authors.forename: Cheryl A
authors.initials: CA
authors.lastname: Kerfeld
authors.forename: Stephanie
authors.initials: S
authors.lastname: Yoshida
authors.forename: Kimberlee T
authors.initials: KT
authors.lastname: Tran
authors.forename: Todd O
authors.initials: TO
authors.lastname: Yeates
authors.forename: Duilio
authors.initials: D
authors.lastname: Cascio
authors.forename: Hervé
authors.initials: H
authors.lastname: Bottin
authors.forename: Catherine
authors.initials: C
authors.lastname: Berthomieu
authors.forename: Miwa
authors.initials: M
authors.lastname: Sugiura
authors.forename: Alain
authors.initials: A
authors.lastname: Boussac
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry; D018360:Crystallography, X-Ray; D000458:Cyanobacteria / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D006863:Hydrogen-Ion Concentration; D011487:Protein Conformation; D016415:Sequence Alignment; D013482:Superoxide Dismutase / Q000737:chemistry*; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D013482:Superoxide Dismutase