Characterization of DNA, RNA and DNA/RNA duplexes containing an L-nucleotide.
In order to investigate the duplex structure of DNA and RNA containing an L-nucleotide residue, we carried out the UV-melting and CD experiments. Although the introduction of an L-nucleotide into DNA/DNA, DNA/RNA and RNA/RNA duplexes reduced their Tm values, the typical two-state transitions were observed for all of the duplexes. The incorporation of an L-nucleotide into the RNA strand caused more remarkable decreases of the Tm value than that into the DNA strand. The CD spectra of the DNA/DNA and RNA/RNA duplexes containing an L-nucleotide are similar to those of unmodified duplexes. On the other hand, the spectra of the heterochiral DNA/RNA hybrid duplexes are significantly different from those of the homochiral DNA/RNA hybrid duplexes, which are generally thought to adopt the A-form.
Urata
H
H
Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
Shimizu
H
H
Hiroaki
H
H
Kohda
D
D
Akagi
M
M
D001483:Base Sequence; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D007536:Isomerism; D046911:Macromolecular Substances; D009690:Nucleic Acid Conformation; D009711:Nucleotides / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D013696:Temperature
D046911:Macromolecular Substances; D009711:Nucleotides; D012313:RNA; D004247:DNA