Diagnosis of disorders of platelet function by flow cytometry.
The platelet membrane glycoprotein (GP) IIb/IIIa, GPIX were detected by immunogold assay and flow cytometry, respectively, in two cases of Glannzmann's thrombasthenia (GT). The immunogold assay showed that the GP IIb/IIIa gold granules in GT were decreased obviously, compared with that in normal controls. Flow cytometry results showed that GP IIb/IIIa in two cases of GT was 0.1% and 0.5% of normal control, respectively. While GPIX showed no difference between the GT and normal control. The patients' father showed no bleeding symptoms, whose GP IIb/IIIa accounted for 36.99% of normal control. The experiment results suggested that flow cytometry is a quick, simple and sensitive method for the diagnosis of GP disorders.
Hu
Y
Y
Institute of Hematology, Xiehe Hospital, Tongji Medical University, Wuhan 430022.
Huang
R
R
Chen
Z
Z
Wei
W
W
D000328:Adult; D001792:Blood Platelets / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000378:metabolism*; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000378:metabolism*; D013915:Thrombasthenia / Q000097:blood* / Q000175:diagnosis
D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D019038:Platelet Glycoprotein GPIb-IX Complex