SIR-dependent repression of non-telomeric genes in Saccharomyces cerevisiae?
The proteins Sir2, Sir3 and Sir4 repress transcription of the silent mating-type loci HML and HMR and of reporter genes inserted at telomeres. Previous microarray analyses suggested that additional non-telomeric genes exist which are repressed by the Sir proteins. In this study, we tested the expression of 12 such genes by Northern analysis and RT-PCR. However, we were unable to verify their SIR-dependent regulation, which suggests that SIR-mediated repression may be restricted to the known repressed regions.
Marchfelder
Uta
U
Otto-Warburg-Laboratories, Max-Planck-Institut für Molekulare Genetik, D-14195 Berlin, Germany.
Rateitschak
Katja
K
Ehrenhofer-Murray
Ann E
AE
D015152:Blotting, Northern; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal / Q000235:genetics*; D049770:Genes, Mating Type, Fungal; D012331:RNA, Fungal / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae / Q000235:genetics*; D016615:Telomere / Q000235:genetics*; D014158:Transcription, Genetic
D012331:RNA, Fungal; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae