Alteration and significance of heparin-binding epidermal-growth-factor-like growth factor in psoriatic epidermis.
BACKGROUND
Heparin-binding epidermal-growth-factor-like growth factor (HB-EGF) has proved to be a mitogen of keratinocytes, which may be involved in the pathogenesis of inflammatory diseases, but its mechanism in psoriasis remains unknown.


OBJECTIVE
To investigate the alteration of HB-EGF in the epidermis of active psoriasis vulgaris.


METHODS
The expression of HB-EGF in normal skin tissues, uninvolved tissues and psoriatic lesions was detected by in situ hybridization and immunohistochemistry.


RESULTS
HB-EGF mRNA and protein were located in the basal layer of normal epidermis (10/10, 100.00%), with nearly no expression in the suprabasal layers (1/10, 10.00%); the expression of HB-EGF was located not only in the basal layer (21/21, 100.00%), but was also seen as focal overexpression in the suprabasal layers of uninvolved epidermis (20/21, 95.24%) and the marginal part of psoriatic lesions (18/21, 85.71%), while nearly no expression of HB-EGF was found in the central part of psoriatic lesions (1/21, 4.76%).


CONCLUSIONS
HB-EGF may play an important role in the early pathogenesis of psoriasis.
Zheng
Yan
Y
Institute of Immunopathology, Xi'an Jiaotong University, Xi'an, Shaanxi, PR China.
Peng
Zhenhui
Z
Wang
Yili
Y
Tan
Shengshun
S
Xi
Yanping
Y
Wang
Guorong
G
D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D001707:Biopsy, Needle; D016022:Case-Control Studies; D004815:Epidermal Growth Factor / Q000235:genetics*; D005260:Female; D005819:Genetic Markers / Q000235:genetics; D020022:Genetic Predisposition to Disease; D066257:Heparin-binding EGF-like Growth Factor; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D036341:Intercellular Signaling Peptides and Proteins; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011379:Prognosis; D011565:Psoriasis / Q000235:genetics* / Q000473:pathology*; D012333:RNA, Messenger / Q000032:analysis; D012016:Reference Values; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index
D005819:Genetic Markers; C587883:HBEGF protein, human; D066257:Heparin-binding EGF-like Growth Factor; D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D004815:Epidermal Growth Factor