Antitumor activities of heteropolysaccharides of Poria cocos mycelia from different strains and culture media.
Ten water-soluble heteropolysaccharide fractions were isolated from Poria cocos mycelia cultured from one wild and one cultivated strain in two identical culture media differing only in one component: either corn steep liquor or bran extract. The chemical compositions, including monosaccharide profile, protein content, and molecular mass M(w) of the mycelial polysaccharides were determined. Both the in vitro and in vivo antitumor activities of the heteropolysaccharides were evaluated and compared. The heteropolysaccharides from Poria cocos mycelia cultured with the wild strain in a medium containing corn steep liquor exhibited the highest antitumor activities against Sarcoma 180 in vivo.
Jin
Yong
Y
Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
Zhang
Lina
L
Zhang
Mei
M
Chen
Li
L
Cheung
Peter Chi Keung
PC
Oi
V E C
VE
Lin
Yulu
Y
D000818:Animals; D000970:Antineoplastic Agents / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor / Q000187:drug effects; D002522:Chlorocebus aethiops; D003470:Culture Media / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D018922:HL-60 Cells / Q000187:drug effects; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D025282:Mycelium / Q000737:chemistry* / Q000187:drug effects; D020072:Polyporales / Q000737:chemistry* / Q000187:drug effects; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D012510:Sarcoma 180 / Q000188:drug therapy; D014709:Vero Cells / Q000187:drug effects
D000970:Antineoplastic Agents; D003470:Culture Media; D011134:Polysaccharides