title: Prophenol oxidase A3 in Drosophila melanogaster: activation and the PCR-based cDNA sequence.
abstract: Phenol oxidase exists in Drosophila hemolymph as a prophenol oxidase, A1 and A3, that is activated in vivo with a native activating system, AMM-1, by limited proteolysis with time. The polypeptide in purified prophenol oxidase A3 has a molecular weight of approximately 77,000 Da. A PCR-based cDNA sequence coding A3 has 2501 bp encoding an open reading frame of 682 amino acid residues. The potential copper-binding sites, from Trp-196 to Tyr-245, and from Asn-366 to Phe-421, are highly homologous to the corresponding sites in other invertebrates. The availability of prophenol oxidase cDNA should be useful in revealing the biochemical differences between A1 and A3 isoforms in Drosophila melanogaster that are refractory or unable to activate prophenol oxidase.
authors.affiliation: Biological Laboratory, Faculty of Science, Okayama University of Science, Okayama 700-0005, Japan. asada@das.ous.ac.jp
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Asada
authors.forename: Genta
authors.initials: G
authors.lastname: Yokoyama
authors.forename: Nobuko
authors.initials: N
authors.lastname: Kawamoto
authors.forename: Shigemi
authors.initials: S
authors.lastname: Norioka
authors.forename: Takashi
authors.initials: T
authors.lastname: Hatta
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004156:Catechol Oxidase / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics*; D004331:Drosophila melanogaster / Q000201:enzymology* / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D004792:Enzyme Precursors / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D007527:Isoenzymes / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D004792:Enzyme Precursors; D007527:Isoenzymes; C038451:pro-phenoloxidase; D004156:Catechol Oxidase; D010450:Endopeptidases