RELP, a novel human REG-like protein with up-regulated expression in inflammatory and metaplastic gastrointestinal mucosa.
We screened expressed sequence tag databases for genes with up-regulated expression in inflammatory bowel diseases. A gene encoding a regenerating protein (REG)-like protein called RELP was identified and characterized. The relp gene encodes a major transcript of 1518 nucleotides, and two truncated splice variants. Unlike the reg genes, which form a cluster in chromosome 2, relp maps to chromosome 1p12-13.1. The predicted translation product is a 158-amino acid preprotein, showing 43% to 47% similarity to the REG proteins. It contains a 22-amino acid signal peptide, and a conserved calcium-dependent carbohydrate-recognition domain. Complementary DNA for the orthologous mouse gene was also cloned. The RELP protein is constitutively expressed in epithelial neuroendocrine cells of the small intestine and in parietal cells of the gastric mucosa. An up-regulated expression of RELP was seen in epithelial cells of inflammatory mucosa in ulcerative colitis and Crohn's disease, in regenerating epithelial borders of gastric ulcers, and in metaplastic epithelium in the antrum and the esophagus. Our findings suggest that RELP might be involved in inflammatory and metaplastic responses of the gastrointestinal epithelium.
Kämäräinen
Meerit
M
Department of Pathology, Haartman Institute, University of Helsinki, and Helsinki University Central Hospital Laboratory Diagnostics, Helsinki, Finland.
Heiskala
Kukka
K
Knuutila
Sakari
S
Heiskala
Marja
M
Winqvist
Ola
O
Andersson
Leif C
LC
D000818:Animals; D001483:Base Sequence; D002878:Chromosomes, Human, Pair 1; D030541:Databases, Genetic; D020224:Expressed Sequence Tags; D005753:Gastric Mucosa / Q000166:cytology / Q000473:pathology / Q000502:physiology*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015212:Inflammatory Bowel Diseases / Q000235:genetics / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000166:cytology / Q000473:pathology / Q000502:physiology*; D037181:Lectins, C-Type; D008679:Metaplasia; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D000073718:Pancreatitis-Associated Proteins; D014176:Protein Biosynthesis; D016415:Sequence Alignment; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured; D015854:Up-Regulation / Q000502:physiology*
D037181:Lectins, C-Type; D009419:Nerve Tissue Proteins; D000073718:Pancreatitis-Associated Proteins; C428528:REG4 protein, human