The prohibitin 3' untranslated region polymorphism is not associated with risk of ovarian cancer.
OBJECTIVE
The 3' untranslated region of the prohibitin gene encodes an RNA molecule that arrests cell proliferation between the G(1) and S phases of the cell cycle, and a C-to-T transition within this region creates a variant lacking antiproliferative activity. The T allele was reported to be associated with an increased risk of breast cancer in North American women, specifically in those under age 50 years reporting a first-degree family history of breast cancer. We assessed the association of the prohibitin 3' untranslated region polymorphism with risk of ovarian cancer in the Australian population by case-control comparison.


METHODS
We examined 553 cases of epithelial ovarian cancer and 300 unaffected controls to assess whether this polymorphism was associated with risk of ovarian cancer in Australian women. Genotyping was carried out using the Perkin-Elmer ABI Prism 7700 Sequence Detection System for fluorogenic polymerase chain reaction allelic discrimination. Genotype distributions were compared by logistic regression.


RESULTS
Stratification of the ovarian cancer cases according to tumour behavior (low malignant potential or invasive), histology, grade, stage, or p53 immunohistochemical status failed to reveal any heterogeneity with respect to prohibitin genotype. There was no difference in genotype distribution between cases and controls, with an odds ratio (95% confidence interval) of 0.99 (0.72-1.35) for the CT/TT genotype.


CONCLUSIONS
The prohibitin T variant does not appear to be associated with risk of ovarian cancer in Australian women.
Spurdle
Amanda B
AB
Cancer and Cell Biology Division, The Queensland Institute of Medical Research, Herston, PO Royal Brisbane Hospital, Queensland 4029, Australia. mandyS@qimr.edu.au
Purdie
David M
DM
Chen
Xiaoqing
X
Chenevix-Trench
Georgia
G
D020413:3' Untranslated Regions / Q000235:genetics; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000235:genetics*; D011110:Polymorphism, Genetic; D000091402:Prohibitins; D011506:Proteins / Q000235:genetics*; D012097:Repressor Proteins; D012307:Risk Factors
D020413:3' Untranslated Regions; D000091402:Prohibitins; D011506:Proteins; D012097:Repressor Proteins