Developing human erythroid cells grown in transferrin-free medium utilize iron originating from extracellular ferritin.
In addition to transferrin, ferritin can also function as a source of iron for heme synthesis (Gelvin D, et al. Blood 1996;88:3200-3207; Meyron-Holtz EG, et al. Blood 1999;94:3205-3211). In the present study we investigate the effect of external ferritin on the proliferation and hemoglobinization of human erythroid precursors grown in transferrin-free cultures.
Leimberg
J M
JM
Department of Human Nutrition and Metabolism, Faculty of Medicine, The Hebrew University, Jerusalem, Israel.
Konijn
A M
AM
Fibach
E
E
D018929:Cell Culture Techniques / Q000379:methods; D016895:Culture Media, Serum-Free; D015672:Erythroid Precursor Cells / Q000166:cytology* / Q000378:metabolism; D005260:Female; D005293:Ferritins / Q000378:metabolism*; D006801:Humans; D007501:Iron / Q000378:metabolism*; D010920:Placenta; D011247:Pregnancy; D012016:Reference Values; D014168:Transferrin
D016895:Culture Media, Serum-Free; D014168:Transferrin; D005293:Ferritins; D007501:Iron