Anti-oxidant effect of flavonoids on the susceptibility of LDL oxidation.
In vitro studies have demonstrated increased atherogenicity of oxidized low-density lipoprotein (ox-LDL) compared to native LDL. Oxidative modification of LDL alters its structure allowing LDL to be taken up by scavenger receptors on macrophage, endothelial, and smooth muscle cells, leading to the formation of lipid-laden foam cells, the hallmark of early atherosclerotic lesions. The susceptibility of LDL to in vitro oxidation was assessed essentially by the technique described by Esterbauer et al. LDL oxidation were monitored by change in 234-absorbance in the presence and absence of pure flavonoids. Morin, genistein, apigenin and biochanin A, naringin and quercetin were used at different concentration. These flavonoids significantly inhibit in vitro LDL oxidation, genistein, morin and naringin have stronger inhibitory activity against LDL oxidation than biochanin A or apigenin. This study show that flavonoids prevent in vitro LDL oxidation and probably would be important to prevent atherosclerosis.
Naderi
Gholam Ali
GA
Department of Biochemistry, Isfahan Cardiovascular Research Center, Amin Hospital, Isfahan University of Medical Sciences, Isfahan, Iran. isfcarvasrc@hotmail.com
Asgary
Seddigheh
S
Sarraf-Zadegan
Nizal
N
Shirvany
Hamid
H
D000975:Antioxidants / Q000494:pharmacology*; D047310:Apigenin; D001161:Arteriosclerosis / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control; D005419:Flavonoids / Q000494:pharmacology*; D019833:Genistein / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D008077:Lipoproteins, LDL / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism*; D010084:Oxidation-Reduction; D047309:Flavones
D000975:Antioxidants; D047310:Apigenin; D005419:Flavonoids; D019833:Genistein; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; C008548:morin; D047309:Flavones; C004541:biochanin A