Improved adaptation to cold-shock, stationary-phase, and freezing stresses in Lactobacillus plantarum overproducing cold-shock proteins.
We have investigated the effect of overproducing each of the three cold shock proteins (CspL, CspP, and CspC) in the mesophilic lactic acid bacterium Lactobacillus plantarum NC8. CspL overproduction transiently alleviated the reduction in growth rate triggered by exposing exponentially growing cells to cold shock (8 degrees C), suggesting that CspL is involved in cold adaptation. The strain overproducing CspC resumed growth more rapidly when stationary-phase cultures were diluted into fresh medium, indicating a role in the adaptation and recovery of nutritionally deprived cells. Overproduction of CspP led to an enhanced capacity to survive freezing.
Derzelle
Sylviane
S
Institut des Sciences de la Vie/Unité de Génétique, Université catholique de Louvain, 5 Place Croix du Sud, B-1348 Louvain-la-Neuve, Belgium.
Hallet
Bernard
B
Ferain
Thierry
T
Delcour
Jean
J
Hols
Pascal
P
D000222:Adaptation, Physiological; D001426:Bacterial Proteins / Q000378:metabolism; D003080:Cold Temperature; D005615:Freezing; D015964:Gene Expression Regulation, Bacterial; D006360:Heat-Shock Proteins / Q000378:metabolism*; D018869:Heat-Shock Response; D007778:Lactobacillus / Q000254:growth & development / Q000502:physiology*
D001426:Bacterial Proteins; C088099:CspC protein, bacteria; C105798:CspL protein, Lactobacillus plantarum; C105800:CspP protein, Lactobacillus plantarum; D006360:Heat-Shock Proteins