Expression of adherent molecule and cyclin by ligustrazine in bone marrow of mice with immune-induced aplastic anemia.
Mice with immune-induced aplastic anemia (AA) were given 5 mg ligustrazine intraperitoneally twice a day. On the 14th day, the expression of CD49d, CD49c, cyclinD2 in bone marrow mononuclear cells (MNC) was examined by flow cytometry, and VCAM-1 on stromal cells was immunohistochemically measured by Strept Avidin-Biotin Complex (SABC). The expression of CD49d, CD49c, VCAM-1 and cyclinD2 in ligustrazine-treated group was significantly higher than that in AA group (P < 0.01), but the ratio of G0 + G1 phase cells was significantly lower than that in AA group (P < 0.01). The results showed that ligustrazine could improve the expression of adherent molecule and cyclin D2 in the bone marrow of mice with immune-induced aplastic anemia, thereby promoting the growth of hematopoietic cells.
Dong
L
L
Department of Hematology, Tongji Hospital, Tongji Medical University, Wuhan 430030.
Sun
H
H
Liu
W
W
Xu
H
H
Tao
D
D
D000741:Anemia, Aplastic / Q000209:etiology / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D018952:Antigens, CD34 / Q000096:biosynthesis* / Q000235:genetics; D001853:Bone Marrow / Q000378:metabolism; D056742:Cyclin D2; D016213:Cyclins / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D039422:Integrin alpha3 / Q000096:biosynthesis* / Q000235:genetics; D039441:Integrin alpha4 / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D011719:Pyrazines / Q000494:pharmacology*; D011897:Random Allocation; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis / Q000235:genetics
D000911:Antibodies, Monoclonal; D018952:Antigens, CD34; C486196:Ccnd2 protein, mouse; D056742:Cyclin D2; D016213:Cyclins; D039422:Integrin alpha3; D011719:Pyrazines; D019010:Vascular Cell Adhesion Molecule-1; D039441:Integrin alpha4; C017953:tetramethylpyrazine