Molecular design of hairpin imidazole-pyrrole polyamide for effective DNA alkylation.
A new type of hairpin polyamide-CPI conjugate possessing a vinyl linker has been synthesized. Sequence-selective alkylation of double-stranded DNA by conjugates was investigated by high resolution denaturing gel electrophoresis using 450 bp DNA fragments. Highly-efficient DNA alkylation predominantly occurs at the purines of 5'-(A/T)G(T/A)CPu-3' site at nanomolar concentration.
Bando
T
T
CREST, Japan Science and Technology Corporation (JST), Japan.
Narita
A
A
Saito
I
I
Sugiyama
H
H
D000478:Alkylation; D004247:DNA / Q000737:chemistry*; D007093:Imidazoles / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D009757:Nylons / Q000138:chemical synthesis* / Q000737:chemistry*; D011687:Purines / Q000737:chemistry; D011758:Pyrroles / Q000737:chemistry*
D007093:Imidazoles; D009757:Nylons; D011687:Purines; D011758:Pyrroles; D004247:DNA; C096654:N-methylpyrrole; C018100:1-methylimidazole