MCP-1 promoter polymorphism in Spanish patients with rheumatoid arthritis.
To investigate the possible role of the polymorphism located in the regulatory region of monocyte chemoattractant protein-1 (MCP-1) gene in the susceptibility to rheumatoid arthritis (RA), a total of 141 Spanish RA patients and 194 controls, previously typed for human leukocyte antigen DRB1* (HLA-DRB1*), were genotyped for -2518 (A/G) MCP-1 gene polymorphism using polymerase chain reaction-restriction fragment length polymorphism. No association between -2518 (A/G) MCP-1 polymorphism and susceptibility to RA was found. Nevertheless, when patients and controls were stratified according to their HLA shared epitope (SE) status, a significant increase in the frequency of genotype GG was found among SE negative (SE-) patients with respect to both SE positive (SE+) patients and SE- controls (16% versus 4% in SE+ patients, pFisher=0.04, odds ratio [OR]=4.4, 95% confidence interval [95%CI]=1.03-21.48; and 4% in SE- controls, pFisher=0.02, OR=4.13, 95%CI=1.10-15.72). In conclusion, MCP-1 polymorphism is slightly associated with the susceptibility to RA in patients lacking the HLA SE.
González-Escribano
Maria Francisca
MF
Servicio de Inmunología, Hospital Universitario Virgen del Rocío, Servicio Andaluz de Salud, Sevilla, Spain.
Torres
Belén
B
Aguilar
Francisco
F
Rodríguez
Rosa
R
García
Alicia
A
Valenzuela
Angel
A
Núñez-Roldán
Antonio
A
D000483:Alleles; D001172:Arthritis, Rheumatoid / Q000235:genetics* / Q000276:immunology / Q000503:physiopathology; D018932:Chemokine CCL2 / Q000235:genetics*; D004247:DNA / Q000032:analysis; D017931:DNA Primers; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D006630:Hispanic or Latino; D006801:Humans; D008297:Male; D010641:Phenotype; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D013030:Spain
D018932:Chemokine CCL2; D017931:DNA Primers; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D004247:DNA