title: Effect of chlorogenic acid on hydroxyl radical.
abstract: Chlorogenic acid (CGA) is considered to act as an antioxidant. However, the inhibitory effects of CGA on specific radical species are not well understood. Electron spin resonance (ESR) in combination with spin trapping techniques was utilized to detect free radicals. 5,5-Dimethyl-1-pyrroline-N-oxide (DMPO) was used as a spin trapping reagent while the Fenton reaction was used as a source of hydroxyl radical (*OH). We found that CGA scavenges *OH in a dose-dependent manner. The kinetic parameters, IC50 and Vmax, for CGA scavenging of *OH were 110 and 1.27 microM/sec, respectively. The rate constant for the scavenging of *OH by CGA was 7.73 x 10(9) M(-1) sec(-1). Our studies suggest that the antioxidant properties of CGA may involve a direct scavenging effect of CGA on *OH.
authors.affiliation: Exposure Assessment Branch and Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505-2888, USA. laz7@cdc.gov
authors.forename: Lun-Yi
authors.initials: LY
authors.lastname: Zang
authors.forename: Greg
authors.initials: G
authors.lastname: Cosma
authors.forename: Henry
authors.initials: H
authors.lastname: Gardner
authors.forename: Vince
authors.initials: V
authors.lastname: Castranova
authors.forename: Val
authors.initials: V
authors.lastname: Vallyathan
mesh_terms: D002726:Chlorogenic Acid / Q000737:chemistry / Q000494:pharmacology*; D003497:Cyclic N-Oxides / Q000737:chemistry / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004578:Electron Spin Resonance Spectroscopy; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000737:chemistry*; D020128:Inhibitory Concentration 50; D019097:Spin Trapping
chemical_list: D003497:Cyclic N-Oxides; D016166:Free Radical Scavengers; D002726:Chlorogenic Acid; D017665:Hydroxyl Radical; C017245:5,5-dimethyl-1-pyrroline-1-oxide