Growth factor analysis of low-grade glioma CSF: PDGF and VEGF are not detectable.
Platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF) and fibroblast growth factor 2 (FGF-2) are involved in the development of grade 2 gliomas. The aim of the present study was to determine the presence of these growth factors in the cerebrospinal fluid (CSF) and to assess their usefulness as biological markers. CSF was collected from 7 adult patients with newly diagnosed supratentorial low-grade gliomas by lumbar puncture and was analysed together with matched serum samples using radioreceptor and enzyme-linked immunosorbant assays. Neither PDGF nor VEGF were detected in the CSF, and FGF-2 was measurable at extremely low concentrations in only 2 of 7 patients. Serum levels were within normal limits. We conclude that these growth factors are not released into the CSF in any significant amounts and are therefore not suitable as biological markers in grade 2 gliomas.
Ribom
D
D
Department of Neuroscience, Neurology, University Hospital, University of Uppsala, SE-751 85 Uppsala, Sweden.
Larsson
A
A
Pietras
K
K
Smits
A
A
D000293:Adolescent; D000328:Adult; D001774:Blood Chemical Analysis / Q000379:methods; D001932:Brain Neoplasms / Q000097:blood / Q000134:cerebrospinal fluid; D016228:Endothelial Growth Factors / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005910:Glioma / Q000097:blood / Q000134:cerebrospinal fluid*; D006133:Growth Substances / Q000097:blood / Q000134:cerebrospinal fluid*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis*; D008222:Lymphokines / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D009389:Neovascularization, Pathologic; D010982:Platelet-Derived Growth Factor / Q000032:analysis*; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D016228:Endothelial Growth Factors; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D008222:Lymphokines; D010982:Platelet-Derived Growth Factor; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors