title: Effect of gangliosides on the copper-induced oxidation of human low-density lipoproteins.
abstract: The role of gangliosides in the copper-induced oxidative modification of human low-density lipoprotein (LDL) was studied focusing on the early stage of LDL oxidation in which the concentration of conjugated dienes increases only weakly. The changes in the protein and lipid component were followed using fluorescence spectroscopy. The results indicate that binding of gangliosides to LDL causes slower destruction of tryptophan fluorescence and suppresses cross-linking between the reactive groups of the protein and the products of lipid peroxidation. The protective role of gangliosides could be assigned to their interference with the lipid-protein interaction in the LDL particle, which might be important for the maintenance of the native plasma antioxidant status in vivo.
authors.affiliation: Ruder Bosković Institute, Bijenicka 54, 10000 Zagreb, Croatia. kveder@rudjer.irb.hr
authors.forename: Marina
authors.initials: M
authors.lastname: Kveder
authors.forename: Greta
authors.initials: G
authors.lastname: Pifat
authors.forename: Mirjana
authors.initials: M
authors.lastname: Gavella
authors.forename: Vaskresenija
authors.initials: V
authors.lastname: Lipovac
mesh_terms: D003300:Copper; D005454:Fluorescence Polarization; D005732:Gangliosides / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D008077:Lipoproteins, LDL / Q000737:chemistry* / Q000302:isolation & purification; D010084:Oxidation-Reduction; D014364:Tryptophan / Q000737:chemistry
chemical_list: D005732:Gangliosides; D008077:Lipoproteins, LDL; D003300:Copper; D014364:Tryptophan