title: Multilocus sequence typing of Staphylococcus aureus with DNA array technology.
abstract: A newly developed oligonucleotide array suited for multilocus sequence typing (MLST) of Staphylococcus aureus strains was analyzed with two strain collections in a two-center study. MLST allele identification for the first strain collection fully agreed with conventional strain typing. Analysis of strains from the second collection revealed that chip-defined MLST was concordant with conventional MLST. Array-mediated MLST data were reproducible, exchangeable, and epidemiologically concordant.
authors.affiliation: Department of Medical Microbiology & Infectious Diseases, Erasmus MC, 3015 GD Rotterdam, The Netherlands. vanleeuwen@erasmusmc.nl
authors.forename: Willem B
authors.initials: WB
authors.lastname: van Leeuwen
authors.forename: Corinne
authors.initials: C
authors.lastname: Jay
authors.forename: Susan
authors.initials: S
authors.lastname: Snijders
authors.forename: Nathalia
authors.initials: N
authors.lastname: Durin
authors.forename: Bruno
authors.initials: B
authors.lastname: Lacroix
authors.forename: Henry A
authors.initials: HA
authors.lastname: Verbrugh
authors.forename: Mark C
authors.initials: MC
authors.lastname: Enright
authors.forename: Alain
authors.initials: A
authors.lastname: Troesch
authors.forename: Alex
authors.initials: A
authors.lastname: van Belkum
mesh_terms: D000483:Alleles; D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D006801:Humans; D016106:Methicillin Resistance; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D017422:Sequence Analysis, DNA; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000145:classification* / Q000187:drug effects / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins