Site-selective artificial ribonuclease using pinpoint RNA activation.
An acridine residue attached to the end or the inside of a DNA activates the target phosphodiester linkages in complementary RNA. Due to this pinpoint activation, the RNA is site-selectively and efficiently cleaved at these linkages by various free metal ions under mild condition. Spectroscopic analyses show that the acridine moiety is sandwiched between neighboring bases, and pushes the opposite nucleotide out of the hetero-duplex.
Kuzuya
A
A
Research Center for Advanced Science and Technology, University of Tokyo, Komaba, Meguro-ku, Tokyo 153-8904, Japan.
Mizoguchi
R
R
Komiyama
M
M
D000166:Acridines / Q000737:chemistry; D001483:Base Sequence; D002384:Catalysis; D028581:Lanthanoid Series Elements / Q000737:chemistry; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D012260:Ribonucleases / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism
D000166:Acridines; D028581:Lanthanoid Series Elements; D009838:Oligodeoxyribonucleotides; D012313:RNA; D012260:Ribonucleases