Double stranded RNA virus in South African Trichomonas vaginalis isolates.
OBJECTIVE
To screen Trichomonas vaginalis isolates from South Africa for the presence of a small double stranded RNA virus designated T vaginalis virus (TVV).


METHODS
TVV was detected by simultaneous extraction of DNA and RNA, and its presence confirmed by electron microscopy and nuclease digestions.


RESULTS
TVV was detected in 59 of 72 (81.9%) isolates.


CONCLUSIONS
These results indicate a possible higher infection rate of South African T vaginalis isolates by the double stranded RNA virus than has been reported for isolates elsewhere.
Weber
B
B
Department of Medical Microbiology, Medical University of Southern Africa, PO Box 211, Medunsa 0204, South Africa. weber@anaesthesie.uni-kiel.de
Mapeka
T M
TM
Maahlo
M A
MA
Hoosen
A A
AA
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D008854:Microscopy, Electron; D012328:RNA Viruses / Q000235:genetics / Q000302:isolation & purification*; D012330:RNA, Double-Stranded / Q000032:analysis* / Q000648:ultrastructure; D012367:RNA, Viral / Q000032:analysis* / Q000648:ultrastructure; D013019:South Africa; D014246:Trichomonas vaginalis / Q000821:virology*
D012330:RNA, Double-Stranded; D012367:RNA, Viral