Plant regeneration from callus of apomictic and sexual lines of Paspalum simplex and RFLP analysis of regenerated plants.
Culture conditions have been established for the induction of callus from different explants of Paspalum simplex. Fast-growing calli were obtained from hypocotyls and roots excised from 5-day-old seedlings on culture medium containing 2,4-dichlorophenoxyacetic acid and kinetin. Rapid plant regeneration from both apomictic and sexual lines was achieved when the medium was supplemented with alpha-naphthaleneacetic acid and benzylaminopurine. Restriction fragment length polymorphism analysis of the apomixis-controlling region of the regenerated plants showed an absence of restriction site variation for the loci analysed, whereas various degrees of variation were detected for the DNA methylation sites of the same loci.
Molinari
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L
Institute of Plant Genetics-CNR, Research Division Perugia, Via della Madonna alta 130, 06128, Perugia, Italy.
Busti
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A
Calderini
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O
Arcioni
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Pupilli
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F
D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D003470:Culture Media / Q000737:chemistry; D046508:Culture Techniques; D019175:DNA Methylation; D014644:Genetic Variation; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D031781:Paspalum / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012038:Regeneration / Q000187:drug effects / Q000502:physiology*; D015183:Restriction Mapping
D003470:Culture Media; D009280:Naphthaleneacetic Acids; C034182:1-naphthaleneacetic acid; D000225:Adenine