title: [Use of a genetic system of the chimeric protein P-Ph in the repression of gene transcription of the leg-arista-wing complex in Drosophila].
abstract: Preliminary analysis of the leg-arista-wing complex (lawc) gene region in the corresponding mutants revealed P element insertion in the transcribed region of this locus. To demonstrate the main role of P element insertion in the complex pleiotropic phenotypic manifestation of the lawc gene, a system using P-Ph chimeric protein and based on the P-mediated repression of the lawc in vivo transcription was applied. As a result, extreme lawc-mutant phenotypes were obtained and examined. The P-Ph-mediated decrease of the level of the lawc gene transcription was also demonstrated.
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Modestova
authors.forename: D V
authors.initials: DV
authors.lastname: Kopytova
authors.forename: S G
authors.initials: SG
authors.lastname: Georgieva
authors.forename: O B
authors.initials: OB
authors.lastname: Simonova
mesh_terms: D000483:Alleles; D000818:Animals; D004330:Drosophila / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology*; D017344:Genes, Insect; D016153:Genes, Suppressor; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D016254:Mutagenesis, Insertional; D009154:Mutation; D010641:Phenotype; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D019476:Insect Proteins; D011993:Recombinant Fusion Proteins