X-ray analyses of d(GCGAAAGC) and d(GXGAAAGCT), where X = 2'-deoxy-5-iodocytidine.
DNA fragments containing a sequence d(GCGAAAGC) are known to be highly thermostable. To investigate the structural basis for such a specific property, crystallographic studies of the DNA octamer and a nanomer d(GXGAAAGCT) (X = 2'-deoxy-5-iodocytidine) have been performed. The present higher resolution X-ray analyses have shown that both DNA oligomers are stabilized respectively to form a zipper-type duplex homodimer. PAGE of these oligomers, however, indicates that they are monomeric even when their crystals were dissolved at room temperature.
Sunami
T
T
Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori-ku, Yokohama 226-8501, Japan.
Kondo
J
J
Chatake
T
T
Hirao
I
I
Watanabe
K
K
Miura
K
K
Takénaka
A
A
D001483:Base Sequence; D001972:Bromodeoxycytidine / Q000031:analogs & derivatives; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry*; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000737:chemistry*; D019281:Dimerization; D008958:Models, Molecular; D009690:Nucleic Acid Conformation
D003841:Deoxycytidine; D001972:Bromodeoxycytidine; C011288:ibacitabine; D004247:DNA