Immunohistochemical evaluation of PCNA, p53, HSP60, HSP10 and MUC-2 presence and expression in prostate carcinogenesis.
BACKGROUND
The study of the expression of different biological markers in non-neoplastic, pre-neoplastic and neoplastic lesions of prostate could help to better understand their role in carcinogenesis and to find new diagnostic and prognostic tools.


METHODS
In the present work we evaluated, by immunohistochemistry, the presence and the expression of PCNA, p53, HSP60, HSP10 and MUC-2 in a series of nodular hyperplasia, low- and high-grade prostatic intraepithelial lesions and adenocarcinomas.


RESULTS
Our data confirmed that: 1) PCNA expression could be related to the grade of progression of cancer; and that 2) p53 mutation could be a late event in prostate carcinogenesis. Moreover, we reported that: 1) HPS60 and HPS10 were overexpressed early in prostate carcinogenesis; and that 2) MUC-2 is absent in both tumoral and non-tumoral prostatic tissue.


CONCLUSIONS
We suggest the further examination, by molecular and genetic studies, of the role of HSP60 and HSP10 during carcinogenesis of the prostate as well as of other organs.
Cappello
Francesco
F
Human Anatomy Section, Department of Experimental Medicine, Institute of Pathological Anatomy, University of Palermo, Italy. francapp@hotmail.com
Rappa
Francesca
F
David
Sabrina
S
Anzalone
Rita
R
Zummo
Giovanni
G
D000230:Adenocarcinoma / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D018835:Chaperonin 10 / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D018834:Chaperonin 60 / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D018450:Disease Progression; D020869:Gene Expression Profiling; D016158:Genes, p53; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D055262:Mucin-2; D009077:Mucins / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D009363:Neoplasm Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D011467:Prostate / Q000378:metabolism*; D011470:Prostatic Hyperplasia / Q000378:metabolism*; D019048:Prostatic Intraepithelial Neoplasia / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000032:analysis* / Q000096:biosynthesis
D018835:Chaperonin 10; D018834:Chaperonin 60; C524625:MUC2 protein, human; D055262:Mucin-2; D009077:Mucins; D009363:Neoplasm Proteins; D018809:Proliferating Cell Nuclear Antigen; D016159:Tumor Suppressor Protein p53