Increased susceptibility of striatal mitochondria to calcium-induced permeability transition.
Mitochondria were simultaneously isolated from striatum and cortex of adult rats and compared in functional assays for their sensitivity to calcium activation of the permeability transition. Striatal mitochondria showed an increased dose-dependent sensitivity to Ca2+ compared with cortical mitochondria, as measured by mitochondrial depolarization, swelling, Ca2+ uptake, reactive oxygen species production, and respiration. Ratios of ATP to ADP were lower in striatal mitochondria exposed to calcium despite equal amounts of ADP and ATP under respiring and nonrespiring conditions. The Ca2+-induced changes were inhibited by cyclosporin A or ADP. These responses are consistent with Ca2+ activation of both low and high permeability pathways constituting the mitochondrial permeability transition. In addition to the striatal supersensitivity to induction of the permeability transition, cyclosporin A inhibition was less potent in striatal mitochondria. Immunoblots indicated that striatal mitochondria contained more cyclophilin D than cortical mitochondria. Thus striatal mitochondria may be selectively vulnerable to the permeability transition. Subsequent mitochondrial dysfunction could contribute to the initial toxicity of striatal neurons in Huntington's disease.
Brustovetsky
Nickolay
N
Department of Neuroscience, University of Minnesota, Minneapolis, Minnesota 55455, USA.
Brustovetsky
Tatiana
T
Purl
Kevin J
KJ
Capano
Michela
M
Crompton
Martin
M
Dubinsky
Janet M
JM
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000493:pharmacokinetics / Q000494:pharmacology*; D002540:Cerebral Cortex / Q000737:chemistry; D003342:Corpus Striatum / Q000737:chemistry*; D000081406:Peptidyl-Prolyl Isomerase F; D021983:Cyclophilins / Q000032:analysis / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004579:Electron Transport / Q000187:drug effects / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D006816:Huntington Disease / Q000209:etiology / Q000378:metabolism*; D008027:Light; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D008928:Mitochondria / Q000737:chemistry* / Q000187:drug effects* / Q000378:metabolism; D010539:Permeability / Q000187:drug effects; D051381:Rats; D017382:Reactive Oxygen Species / Q000737:chemistry; D012542:Scattering, Radiation
D000081406:Peptidyl-Prolyl Isomerase F; D004791:Enzyme Inhibitors; D017382:Reactive Oxygen Species; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D021983:Cyclophilins; D002118:Calcium