A pH-indicator-based screen for hydrolytic haloalkane dehalogenase.
Zhao
Huimin
H
Department of Chemical and Biomolecual Engineering, University of Illinois, Urbana, IL, USA.
D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D017931:DNA Primers; D019020:Directed Molecular Evolution / Q000379:methods; D004795:Enzyme Stability; D015723:Gene Library; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006868:Hydrolysis; D007202:Indicators and Reagents; D007700:Kinetics; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012240:Rhodococcus / Q000201:enzymology* / Q000235:genetics; D013816:Thermodynamics; D020584:Xanthobacter / Q000201:enzymology* / Q000235:genetics
D017931:DNA Primers; D007202:Indicators and Reagents; D011994:Recombinant Proteins; D006867:Hydrolases; C046137:haloalkane dehalogenase