title: Raman and IR study on copper binding of histamine.
abstract: A comparative Raman and FTIR study of histamine (Hm), a small hormone present in a wide selection of living organisms, and its complexes with copper(II) at different pH values was carried out. Both the Raman and IR spectra present some marker bands useful for the identification of the structure of the species predominating in the Cu(II) aqueous and alcoholic systems. In particular, Raman spectroscopy appears to be a useful tool for analyzing the tautomeric equilibrium of the imidazole ring of Hm, because some bands (i.e., nuC(4)dbond;C(5)) appear at different wavenumbers, depending on whether the imidazole moiety is in the N(tau)-H (tautomer I) or N(pi)-H (tautomer II) protonated form. In aqueous solutions the manner in which Hm binds to Cu(II) depends on the pH. At basic pH the most relevant species formed are a dimer, [Cu(2)L(2)H(-2)](2+), and a monomeric complex, [CuL](2-) or [CuL(2)](+). On the contrary, by decreasing the pH, Hm acts as a mono- or bidentate ligand, giving rise to two types of monomeric complexes, [CuLH](2-) and [CuL](2-) or [CuL(2)](+). With respect to the Cu(II)-Hm alcoholic system, both the aminic group and the imidazole ring (tautomer I) take part in the Cu(II) coordination, leading to the formation of the [CuL](2-) or [CuL(2)](+) monomeric complex.
authors.affiliation: Istituto I.S.O.F. (C.N.R.), Via P. Gobetti 101, 40129 Bologna, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Torreggiani
authors.forename: M
authors.initials: M
authors.lastname: Tamba
authors.forename: S
authors.initials: S
authors.lastname: Bonora
authors.forename: G
authors.initials: G
authors.lastname: Fini
mesh_terms: D003300:Copper / Q000378:metabolism*; D006632:Histamine / Q000737:chemistry* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D008958:Models, Molecular; D008968:Molecular Conformation; D013055:Spectrophotometry, Infrared; D013059:Spectrum Analysis, Raman
chemical_list: D008024:Ligands; D003300:Copper; D006632:Histamine