[Sensitivities of various cell cultures for the isolation of enteroviruses].
In the present study, the sensitivities of HEp-2 (human epithelioma), RD (rhabdomyosarcoma) and L20B (mouse cells, that have receptors for human polioviruses) cell cultures have been evaluated and compared, for the isolation and identification of enteroviruses from the stool and cerebrospinal fluid samples of patients with acute flask paralysis and aseptic meningitis, which were examined between the years 1999-2000, in Refik Saydam Institute of Hygiene Center, Virology, Tissue Culture and Enterovirus Laboratory. Of a total of 1663 samples, 131 viral strains were isolated, and 120 of them were identified as enteroviruses, and 11 as adenoviruses. The isolation rates of 48 Sabin-like polioviruses from HEp-2, RD and L20B cell lines were found similar, as 83.3%, 87.5% and 91.6%, respectively. All of 47 Echovirus strains were isolated from RD cells, all of 13 Coxsackie type B strains were isolated from HEp-2 cells, and all of 12 non-polio enteroviruses were isolated from RD cells. All of 11 adenovirus strains that have been grown in Hep-2 cells, were thought to be occasionally isolated due to the passage of viruses to gastrointestinal tract, and excreted via stool, thus having no clinical significance for these patients. As a result, it was concluded that, all of these three cell lines and especially L20B were sensitive for polioviruses, RD cell line being more sensitive for Echovirus, and HEp-2 cell line being more sensitive for Coxsackie type B virus strains.
Ozkaya
Etem
E
Refik Saydam Hifzissihha Merkezi Başkanliği, Viroloji Laboratuvari, Ankara.
Korukluoğlu
Gülay
G
Yalçinkaya
Tülay
T
Türkeri
Ayten
A
Atak
Tunca
T
Kubar
Ayhan
A
D000818:Animals; D002460:Cell Line; D004770:Enterovirus / Q000145:classification / Q000302:isolation & purification*; D006801:Humans; D051379:Mice; D012680:Sensitivity and Specificity; D014407:Tumor Cells, Cultured