title: Interaction of cyanine dyes with nucleic acids: XXXI. Using of polymethine cyanine dyes for the visualization of DNA in agarose gels.
abstract: Fifteen polymethine cyanine dyes were studied as fluorescent stains for DNA in electrophoretic gels. Among studied cyanines, two dyes CPent V and CCyan 2-O most effectively visualized covalently closed and linear double-stranded DNA molecules in gels under standard conditions using UV-illumination, green filter and black-and-white photo film. Ethidium bromide was 1.2-1.6 times more effective as compared to cyanine dyes in staining of DNA in the concentration range of 8-18 ng, while studied cyanines were more sensitive to DNA quantity above 50 ng.
authors.affiliation: Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, 154 Zabolotny Street, Kyiv 03143, Ukraine. bohdan@serv.imv.kiev.ua
authors.forename: B P
authors.initials: BP
authors.lastname: Matselyukh
authors.forename: S M
authors.initials: SM
authors.lastname: Yarmoluk
authors.forename: A B
authors.initials: AB
authors.lastname: Matselyukh
authors.forename: V B
authors.initials: VB
authors.lastname: Kovalska
authors.forename: I O
authors.initials: IO
authors.lastname: Kocheshev
authors.forename: D V
authors.initials: DV
authors.lastname: Kryvorotenko
authors.forename: S S
authors.initials: SS
authors.lastname: Lukashov
mesh_terms: D002232:Carbocyanines / Q000737:chemistry*; D004247:DNA / Q000032:analysis / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D004996:Ethidium / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D015394:Molecular Structure; D009696:Nucleic Acids / Q000737:chemistry*; D013194:Staining and Labeling
chemical_list: D002232:Carbocyanines; D005456:Fluorescent Dyes; D009696:Nucleic Acids; D004247:DNA; D004996:Ethidium