Simultaneous determination of seven compounds in snow lotus herb using high-performance liquid chromatography.
A reversed-phase high-performance liquid chromatographic method is developed for the quantitative determination of seven components of Snow Lotus Herb (Saussurea tridactyla Sch.-Bip.ex Hook.f.): umbelliferonglucoside, luteolin-7-O-beta-D-glucoside, rutin, apigenin-7-O-beta-D-glucoside, kaempferol-3-O-beta-D-glucoside, apigenin-7-O-beta-D-rutinoside, and luteolin. Samples are analyzed by means of a reverse-phase column (Diamonsil C(18)) using methanol and water under gradient conditions as the mobile phase for 60 min. This method offers selectivity, accuracy, precision, linearity, and ruggedness, as well as efficiency and ease.
Zhang
Xuehui
X
Institute of Medicinal Plant Development, Chinese Academy of Medical Science and Peking Union Medical College, Xibeiwang, Haidian district, Beijing 100094, China. xuehui@yahoo.com.cn
Yu
Minqian
M
Chen
Jianmin
J
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015203:Reproducibility of Results; D030441:Saussurea / Q000737:chemistry*; D012680:Sensitivity and Specificity