Structure of the exceptionally large nonrepetitive carbohydrate backbone of the lipopolysaccharide of Pectinatus frisingensis strain VTT E-82164.
The structures of the oligosaccharides obtained after acetic acid hydrolysis and alkaline deacylation of the rough-type lipopolysaccharide (LPS) from Pectinatus frisingensis strain VTT E-82164 were analysed using NMR spectroscopy, MS and chemical methods. The LPS contains two major structural variants, differing by a decasaccharide fragment, and some minor variants lacking the terminal glucose residue. The largest structure of the carbohydrate backbone of the LPS that could be deduced from experimental results consists of 25 monosaccharides (including the previously found Ara4NP residue in lipid A) arranged in a well-defined nonrepetitive structure: We presume that the shorter variant with R1 = H represents the core-lipid A part of the LPS, and the additional fragment is present instead of the O-specific polysaccharide. Structures of this type have not been previously described. Analysis of the deacylation products obtained from the LPS of the smooth strain, VTT E-79100T, showed that it contains a very similar core but with one different glycosidic linkage.
Vinogradov
Evgeny
E
Institute for Biological Sciences, National Research Council, Ottawa, ON, Canada. evguenii.vinogradov@nrc-cnrc.gc.ca
Petersen
Bent O
BO
Sadovskaya
Irina
I
Jabbouri
Said
S
Duus
Jens Ø
JØ
Helander
Ilkka M
IM
D019342:Acetic Acid / Q000737:chemistry; D000107:Acetylation; D001421:Bacteria, Anaerobic / Q000737:chemistry* / Q000235:genetics; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006027:Glycosides / Q000737:chemistry; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000737:chemistry*; D008759:Methylglycosides / Q000302:isolation & purification; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular
D006027:Glycosides; D008070:Lipopolysaccharides; D008759:Methylglycosides; C008428:3-O-methyl-6-deoxy-D-talose; D019342:Acetic Acid