title: Bacterial components induce cytokine and intercellular adhesion molecules-1 and activate transcription factors in dermal fibroblasts.
abstract: This study investigated the effect of various structural components of Gram-positive (lipotheichoic acid and protein A) and Gram-negative (porins and lipopolysaccharide) bacteria on human dermal fibroblasts. Fibroblasts are important effector cells which have a potential role in augmenting the inflammatory response in various diseases. In this study we present a profile of TNF-alpha, IL-6 and IL-8, the expression of intercellular adhesion molecules (ICAM-1) and the activation of transcriptional nuclear factor NF-kB and AP-1 in human dermal fibroblasts stimulated by bacterial surface components. Compared to the controls, increased ICAM-1, IL-6 and IL-8 gene expression after stimulation of LPS and porins at 2 and 4 h was more evident than that obtained following stimulation of LTA and PA. Gene expression was also associated with the production of cytokine proteins in culture supernatants. TNF-alpha gene expression remained undetectable. Moreover, LPS and porin treatments determined IkBalpha phosphorylation and degradation in human dermal fibroblasts and the subsequent activation of nuclear factors NF-kB and AP-1. These data suggest the importance of such stimuli in the first step of the inflammatory process, as well as the important role played by fibroblasts in skin inflammatory disease.
authors.affiliation: Faculty of Medicine and Surgery, Second University of Naples, Department of Sperimental Medicine, Microbiology and Clinical Microbiology, Via Costantinopoli 16, 80138, Naples, Italy
authors.forename: Brunella
authors.initials: B
authors.lastname: Perfetto
authors.forename: Giovanna
authors.initials: G
authors.lastname: Donnarumma
authors.forename: Daniela
authors.initials: D
authors.lastname: Criscuolo
authors.forename: Iole
authors.initials: I
authors.lastname: Paoletti
authors.forename: Elena
authors.initials: E
authors.lastname: Grimaldi
authors.forename: Maria Antonietta
authors.initials: MA
authors.lastname: Tufano
authors.forename: Adone
authors.initials: A
authors.lastname: Baroni
mesh_terms: D000328:Adult; D019898:Autocrine Communication; D002448:Cell Adhesion / Q000187:drug effects; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000382:microbiology; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D020672:I-kappa B Proteins / Q000378:metabolism; D007249:Inflammation; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis* / Q000235:genetics; D015850:Interleukin-6 / Q000096:biosynthesis / Q000235:genetics; D016209:Interleukin-8 / Q000096:biosynthesis / Q000235:genetics; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008214:Lymphocytes / Q000166:cytology; D008875:Middle Aged; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000378:metabolism*; D019899:Paracrine Communication; D010766:Phosphorylation / Q000187:drug effects; D018272:Porins / Q000302:isolation & purification / Q000494:pharmacology*; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D011550:Pseudomonas aeruginosa / Q000737:chemistry; D012867:Skin / Q000166:cytology; D013205:Staphylococcal Protein A / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000737:chemistry; D013682:Teichoic Acids / Q000494:pharmacology*; D018808:Transcription Factor AP-1 / Q000378:metabolism*; D014158:Transcription, Genetic; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics
chemical_list: D016207:Cytokines; D020672:I-kappa B Proteins; D015850:Interleukin-6; D016209:Interleukin-8; D008070:Lipopolysaccharides; D016328:NF-kappa B; C000606204:NFKBIA protein, human; D018272:Porins; D013205:Staphylococcal Protein A; D013682:Teichoic Acids; D018808:Transcription Factor AP-1; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1; D000072000:NF-KappaB Inhibitor alpha; C009900:lipoteichoic acid