CD4+ T cell apoptosis induced by anti-CD4 antibodies.
To explore the inhibitory effects of anti-CD4 human/murine chimeric antibodies on lymphocyte proliferation, CD4+ T cell apoptosis induced by anti-CD4 antibodies was examined. Annexin-V-FITC and PI double stain method was employed to qualitatively and quantitatively determined CD4+ T cell apoptosis induced by anti-CD4 antibodies. Our results showed that anti-CD4 chimeric antibodies could specifically induce CD4+ T cell apoptosis. The ability of anti-CD4 chimeric antibodies to induce CD4+ T cell apoptosis was related with the presence of monocytes. It is concluded that the further cross-linking of anti-CD4 antibodies is important for inducing CD4+ T cell apoptosis.
Zhang
Z
Z
Department of Immunology, Tongji Medical University, Wuhan 430030.
Zhang
Y
Y
Zhu
H
H
Yang
J
J
Shen
G
G
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D017209:Apoptosis; D015704:CD4 Antigens / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D003432:Cross-Linking Reagents; D004247:DNA / Q000378:metabolism; D006801:Humans; D008213:Lymphocyte Activation; D051379:Mice; D011993:Recombinant Fusion Proteins / Q000276:immunology
D000906:Antibodies; D015704:CD4 Antigens; D003432:Cross-Linking Reagents; D011993:Recombinant Fusion Proteins; D004247:DNA