Phylogenetic analysis of the mammalian Hoxc8 non-coding region.
The non-coding intergenic regions of Hox genes are remarkably conserved among mammals. To determine the usefulness of this sequence for phylogenetic comparisons, we sequenced an 800-bp fragment of the Hoxc9-Hoxc8 intergenic region from several species belonging to different mammalian clades. Results obtained from the phylogenetic analysis are congruent with currently accepted mammalian phylogeny. Additionally, we found a TC mini satellite repeat polymorphism unique to felines. This polymorphism may serve as a useful marker to differentiate between mammalian species or as a genetic marker in feline matings. This study demonstrates usefulness of a comparative approach employing non-coding regions of Hox gene complexes.
Kim
Chang-Bae
CB
Department of Molecular, Cellular, and Developmental Biology, Yale University, PO Box 208103, New Haven, CT 06520, USA.
Shashikant
Cooduvalli S
CS
Sumiyama
Kenta
K
Wang
Wayne C H
WC
Amemiya
Chris T
CT
Ruddle
Frank H
FH
D000818:Animals; D021901:DNA, Intergenic; D004742:Enhancer Elements, Genetic; D019143:Evolution, Molecular; D014644:Genetic Variation; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D008322:Mammals / Q000235:genetics*; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D021901:DNA, Intergenic; C489815:HOXC8 protein, human; D018398:Homeodomain Proteins; C080041:Hoxc6 protein, mouse