[Preparation of hollow fiber bioreactor for culturing pig hepatocytes].
OBJECTIVE
To study the method of preparing the hollow fiber bioreactor for culturing pig hepatocytes.


METHODS
Hepatocytes were isolated from experimental suckling minipigs by two-step perfusion with collagenase, and seeded onto hollow fiber bioreactor, then cultured with an artificial capillary cell culture system. The albumin-excretion, lidocaine-transforming rate, lactate dehydrogenase (LDH) release and the cell viability in bioreactors were examined.


RESULTS
The porcine albumin could be detected by SDS/PAGE on the 2nd, 4th, 6th day. The rates of lidocaine-transforming ranged from 89.6% to 96.1%. The release of LDH into the culture medium increased from (23.7+/-4.6) U/L to (127.8+/-17.4) U/L (F=39.582, P<0.01) during the experiments, and the viability of pig hepatocytes in hollow fiber bioreactor reduced from 95.8%+/-0.3% to 83.8%+/-4.7% (t=5.135, P<0.01).


CONCLUSIONS
The hollow fiber bioreactor for culturing pig hepatocytes can be prepared by artificial capillary cell culture system, which provides a certain liver-specific function in 1 week.
Wang
Ying-jie
YJ
Institute of Infectious Diseases, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
Liu
Hong-ling
HL
Guo
Hai-tao
HT
Liu
Jun
J
Wen
Hong-wei
HW
Wang
Yu-ming
YM
D000818:Animals; D019149:Bioreactors; D001709:Biotechnology; D002478:Cells, Cultured; D005260:Female; D022781:Hepatocytes / Q000166:cytology*; D008099:Liver / Q000166:cytology / Q000502:physiology; D019164:Liver, Artificial; D008297:Male; D013552:Swine; D013556:Swine, Miniature