The effects of rapid salinity change on in vivo arginine kinase flux in the juvenile blue crab, Callinectes sapidus.
The effect of acclimation salinity and salinity changes on the concentration of high-energy phosphate metabolites and arginine kinase (AK) flux was examined in vivo in juvenile blue crabs using 31P-nuclear magnetic resonance (NMR). Crabs were acclimated for 7 days to a salinity of 5 or 35 per thousand and then placed in a flow apparatus that could sustain the animals while NMR spectra were acquired. Crabs were subjected to either hyperosmotic salinity changes, where an animal acclimated to 5 per thousand was exposed to a salinity of 35 per thousand, or hyposmotic changes, which involved the reciprocal exchange. Neither acclimation salinity nor salinity change had a significant effect on the concentrations of arginine phosphate, inorganic phosphate or ATP. 31P-NMR saturation transfer experiments were used to determine the effect of salinity on the forward and reverse flux of the AK reaction. There was no significant effect of acclimation salinity or salinity change on the flux rate through this reaction. This is in contrast to previous results, which showed that AK flux in isolated muscle was sensitive to prevailing osmotic conditions (Holt and Kinsey, J. Exp. Biol. 205 (2002) 1775-1785). The present study indicates that the integrated osmoregulatory capacity of the intact animal is sufficient to preserve cellular energy status and enzyme function during acute salinity changes.
Kinsey
Stephen T
ST
Department of Biological Sciences, University of North Carolina at Wilmington, 601 South College Road, 28403-5915, Wilmington, NC, USA. kinseys@uncw.edu
Lee
Bailey C
BC
D000064:Acclimatization / Q000502:physiology; D000222:Adaptation, Physiological / Q000502:physiology*; D000818:Animals; D001122:Arginine Kinase / Q000378:metabolism*; D003386:Brachyura / Q000201:enzymology* / Q000502:physiology*; D006458:Hemolymph; D009994:Osmolar Concentration; D010710:Phosphates / Q000032:analysis; D012965:Sodium Chloride / Q000032:analysis*; D013997:Time Factors; D014882:Water-Electrolyte Balance / Q000502:physiology*
D010710:Phosphates; D012965:Sodium Chloride; D001122:Arginine Kinase