title: Expansion of the glycosynthase repertoire to produce defined manno-oligosaccharides.
abstract: Mutant endo-mannanases, in which the catalytic nucleophile has been replaced, function as glycosynthases in the synthesis of manno-oligosaccharides of defined lengths.
authors.affiliation: Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, BC V6T 1Z1, Canada.
authors.forename: Michael
authors.initials: M
authors.lastname: Jahn
authors.forename: Dominik
authors.initials: D
authors.lastname: Stoll
authors.forename: R Antony J
authors.initials: RA
authors.lastname: Warren
authors.forename: Lóránd
authors.initials: L
authors.lastname: Szabó
authors.forename: Pritpal
authors.initials: P
authors.lastname: Singh
authors.forename: Harry J
authors.initials: HJ
authors.lastname: Gilbert
authors.forename: Valérie M A
authors.initials: VM
authors.lastname: Ducros
authors.forename: Gideon J
authors.initials: GJ
authors.lastname: Davies
authors.forename: Stephen G
authors.initials: SG
authors.lastname: Withers
mesh_terms: D002240:Carbohydrate Sequence; D002384:Catalysis; D044602:Cellulases / Q000737:chemistry; D006020:Glycopeptides / Q000096:biosynthesis* / Q000737:chemistry; D006026:Glycoside Hydrolases / Q000737:chemistry*; D006031:Glycosylation; D006863:Hydrogen-Ion Concentration; D008351:Mannans / Q000737:chemistry; D008361:Mannosidases / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000737:chemistry*; D016297:Mutagenesis, Site-Directed; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000737:chemistry; D013997:Time Factors; D014166:Transferases / Q000737:chemistry*
chemical_list: D006020:Glycopeptides; D008351:Mannans; D009097:Multienzyme Complexes; D009844:Oligosaccharides; C023176:transglycosidase enzyme system; C014647:mannobiose; D014166:Transferases; D044602:Cellulases; D006026:Glycoside Hydrolases; D008361:Mannosidases; C119276:endo-beta-mannosidase