Influence of Agrobacterium tumefaciens strain on the production of transgenic peas ( Pisum sativum L.).
We compared the efficiency of two Agrobacterium tumefaciens strains, AGL 1 and KYRT1, for producing transgenic pea plants. KYRT1 is a disarmed strain of Chry5 that has been shown to be highly tumourigenic on soybean. The efficacies of the strains were compared using cotyledon explants from three pea genotypes and two plasmids. The peas were sourced from field-grown plants over three Southern Hemisphere summer seasons. Overall, KYRT1 was found to be on average threefold more efficient than AGL 1 for producing transgenic plants. We suggest that KYRT1 is sensitive to cocultivation temperature as the expected increase in efficiency was not achieved at high laboratory temperatures.
Grant
J E
JE
New Zealand Institute for Crop and Food Research, Private Bag 4704, Christchurch, New Zealand. grantj@crop.cri.nz
Thomson
L M J
LM
Pither-Joyce
M D
MD
Dale
T M
TM
Cooper
P A
PA
D016960:Agrobacterium tumefaciens / Q000235:genetics*; D018532:Pisum sativum / Q000235:genetics*; D030821:Plants, Genetically Modified; D013045:Species Specificity; D014170:Transformation, Genetic