title: HPLC fingerprinting and estimation of the bioactive components of Clutia richardiana L. as a potential hypoglycemic herbal tea.
abstract: Clutia richardiana L. is a traditional medicinal herb with demonstrated hypoglycemic effects. An HPLC method (Luna((R)) C(18) column, gradient elution, UV detection at 220 nm) has been utilized to determine the levels of the major diterpenes of C. richardiana in extracts obtained by using three different solvents. Extraction with the organic solvents methanol and acetonitrile was more efficient than aqueous extraction with hot water, but nine markers could be detected equally in all extracts. The aqueous extract was non-toxic against a number of human cell lines, which is a promising indication for its use as a safe and effective antidiabetic herbal preparation.
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Ehab A
authors.initials: EA
authors.lastname: Abourashed
authors.forename: Markus
authors.initials: M
authors.lastname: Ganzera
authors.forename: Ikhlas A
authors.initials: IA
authors.lastname: Khan
authors.forename: Shabana
authors.initials: S
authors.lastname: Khan
authors.forename: Jaber S
authors.initials: JS
authors.lastname: Mossa
authors.forename: Farouk S
authors.initials: FS
authors.lastname: El-Feraly
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D019665:Euphorbiaceae; D006801:Humans; D007004:Hypoglycemic Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D013662:Tea; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D004224:Diterpenes; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D013662:Tea