title: Vasoactive intestinal peptide (VIP) and VIP mRNA decrease in the cerebral cortex of nNOS knock-out(-/-) mice.
abstract: Although there is much evidence showing that NO regulates the release of VIP in several areas, there is no report about the influence of NO on VIP in the cerebral cortex. We therefore examined changes in VIP expression in the cerebral cortex of nNOS knock-out(-/-) mice using immunohistochemistry and in situ hybridization. The nNOS((-/-)) mice had significantly fewer VIP-immunoreactive neurons than the control mice and the VIP mRNA as well as the VIP-immunoreactivity of the individual neuron was decreased in the nNOS((-/-)) mice. The first demonstration of decrease in VIP expression in the cerebral cortex of nNOS((-/-)) mice may provide useful data for investigating the relation between NO and VIP in the cerebral cortex and the mechanisms of many functions of these two neurotransmitters.
authors.affiliation: Department of Anatomy, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-Gu, Seoul 110-799, South Korea.
authors.forename: Myeung Ju
authors.initials: MJ
authors.lastname: Kim
authors.forename: Kyeung Min
authors.initials: KM
authors.lastname: Joo
authors.forename: Yoon Hee
authors.initials: YH
authors.lastname: Chung
authors.forename: Yun Jung
authors.initials: YJ
authors.lastname: Lee
authors.forename: Jaehyup
authors.initials: J
authors.lastname: Kim
authors.forename: Bong Hee
authors.initials: BH
authors.lastname: Lee
authors.forename: Dong Hoon
authors.initials: DH
authors.lastname: Shin
authors.forename: Kyung Hoon
authors.initials: KH
authors.lastname: Lee
authors.forename: Choong Ik
authors.initials: CI
authors.lastname: Cha
mesh_terms: D000818:Animals; D002452:Cell Count / Q000379:methods; D002540:Cerebral Cortex / Q000033:anatomy & histology / Q000201:enzymology / Q000378:metabolism*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D019001:Nitric Oxide Synthase / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D052248:Nitric Oxide Synthase Type I; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D014660:Vasoactive Intestinal Peptide; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; C496321:Nos1 protein, mouse