Molecular cloning, sequencing and alternative splicing of the acid phosphatase (Acph) gene from Drosophila virilis.
The Acph gene of Drosophila virilis was isolated from a genomic library and sequenced. The gene was organized into 6 exons interrupted by 5 introns. 5' and 3' RACE identified 3 transcripts ascribed to alternative splicing. One of them coded for 435 amino acid residues; and the others, for 403 amino acids. The shorter ones lacked 34 residues at the C-terminus end encoded by exon 6, which has been implicated in anchoring the protein to the lysosomal membrane.
Kitagawa
Hiroko T
HT
Department of Chemistry, Faculty of Science, Josai University, Keyakidai 1-1, Sakado, Saitama 350-0295, Japan. hkita@josai.ac.jp
D000135:Acid Phosphatase / Q000096:biosynthesis* / Q000235:genetics*; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002462:Cell Membrane / Q000378:metabolism; D003001:Cloning, Molecular; D004330:Drosophila / Q000235:genetics* / Q000378:metabolism; D005091:Exons; D007438:Introns; D008247:Lysosomes / Q000378:metabolism; D008957:Models, Genetic; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D012333:RNA, Messenger; D000135:Acid Phosphatase