Hydrogen peroxide biosensor based on microperoxidase-11 entrapped in lipid membrane.
A highly catalytic activity microperoxidase-11 (MP-11) biosensor for H(2)O(2) was developed to immobilizing the heme peptide in didodecyldimethylammonium bromide (DDAB) lipid membrane. The enzyme electrode thus obtained responded to H(2)O(2) without electron mediator or promoter, at a potential of +0.10 V versus Agmid R:AgCl. A linear calibration curve is obtained over the range from 2.0 x 10(-5) to 2.4 x 10(-3) M. The biosensor responds to hydrogen peroxide in 15 s and has a detection limit of 8 x 10(-7) M (S/N=3) Providing a natural environment with lipid membrane for protein immobilization and maintenance of protein functions is a suitable option for the design of biosensors.
Huang
Weimin
W
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.
Jia
Jianbo
J
Zhang
Zheling
Z
Han
Xiaojun
X
Tang
Jilin
J
Wang
Jianguo
J
Dong
Shaojun
S
Wang
Erkang
E
D015374:Biosensing Techniques; D004566:Electrodes; D006861:Hydrogen Peroxide / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D008567:Membranes, Artificial; D010544:Peroxidases / Q000737:chemistry / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D013997:Time Factors
D008081:Liposomes; D008567:Membranes, Artificial; D000644:Quaternary Ammonium Compounds; C046112:didodecyldimethylammonium; D006861:Hydrogen Peroxide; D010544:Peroxidases; C021799:microperoxidase