Single-cell irradiation from [211At] astatine-labeled C215 monoclonal antibody: improved estimates of radiosensitivity from measurements on cellular uptake and retention.
New data on the biological effect of 211At-C215 monoclonal antibody in a slowly rotating, widely dispersed single-cell suspension of the human cancer cell line Colo-205 is presented. Cell growth curves of each experiment were used to calculate an apparent cell survival after irradiation. Uptake measurements provided the data needed to calculate the average number of 211At decays per cell in the cell suspension. The results from each experiment were then fit to a mono-exponential function. From the exponential fit, an average of 35 +/- 2 (SD) astatine-211 decays per cell are required for 37% apparent cell survival (D0).
Palm
Stig
S
Department of Radiation Physics, Göteborg University, Sahlgrenska University Hospital, SE-413 45 Göteborg, Sweden. palms@mskcc.org
Bäck
Tom
T
Claesson
Ingela
I
Delle
Ulla
U
Hultborn
Ragnar
R
Lindegren
Sture
S
Jacobsson
Lars
L
D000230:Adenocarcinoma / Q000473:pathology*; D000512:Alpha Particles; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D001246:Astatine / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods; D002470:Cell Survival / Q000528:radiation effects; D003110:Colonic Neoplasms / Q000473:pathology*; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D007553:Isotope Labeling / Q000379:methods; D018499:Linear Energy Transfer; D011836:Radiation Tolerance; D012399:Rotation; D013535:Suspensions; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000528:radiation effects
D000911:Antibodies, Monoclonal; D013535:Suspensions; D001246:Astatine