title: [The protection of Tiron to the injury of organ of Corti cultured in vitro caused by exogenous H2O2 in mouse].
abstract: OBJECTIVE
  In order to observe the action of H2O2 on inner and outer hair cells of organ of Corti in mouse, and discuss whether Tiron can effectively resistant the injury of free radicals in cultured cochlea in vitro or not.
  
  
  METHODS
  Using the culture technology of organ of Corti in newborn mouse in vitro, established the model of the injury of inner and outer hair cells caused by exogenous H2O2. And observed the protection to the damage by H2O2 at different concentration of Tiron.
  
  
  RESULTS
  When the concentration of H2O2 is higher than 1.0 mmol/L, the loss of hair cells in the bottom, middle, and parietal turn of basilar membrane vary, the injury of bottom turn is more severe. In lower than 0.5 mmol/L group, the injury of hair cells is no relationship with the location. Adding Tiron (10 mmol/L) in culture can apparently decrease the injury of hair cells caused by H2O2. When the concentration of H2O2 is 0.01-1.0 mmol/L, Tiron can almost inhibit the loss of hair cells completely.
  
  
  CONCLUSIONS
  There is apparently preventive effection of Tiron on organ of Corti cultured in vitro, and the mechanism of inhibiting injury caused by H2O2 is probably by clearing O2- and combining irony ion.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, First Hospital of Jilin University, Jilin 130021.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Jin
authors.forename: Dalian
authors.initials: D
authors.lastname: Ding
authors.forename: R
authors.initials: R
authors.lastname: Salvi
authors.forename: Baodong
authors.initials: B
authors.lastname: Du
mesh_terms: D014013:1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt / Q000494:pharmacology*; D000818:Animals; D005260:Female; D006198:Hair Cells, Auditory / Q000187:drug effects* / Q000293:injuries; D006861:Hydrogen Peroxide / Q000633:toxicity; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D009925:Organ of Corti / Q000187:drug effects* / Q000293:injuries
chemical_list: D014013:1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt; D006861:Hydrogen Peroxide