Isolation of chlorinated fatty acid methyl esters derived from cell-culture medium and from fish lipids by using an aminopropyl solid-phase extraction column.
An aminopropyl-based solid-phase extraction technique was used for isolation of chlorinated fatty acids in lipids. A range of different chlorinated fatty acids was eluted in a small volume of solvent (4 ml) and the recoveries of the different species and isomers were quantitative. Only 1% of the vastly dominating unchlorinated fatty acid methyl esters were recovered in the fractions containing the chlorinated fatty acid methyl esters. This method makes it possible to isolate and detect > or = 1 microg of a chlorinated fatty acid methyl ester in 1 g of lipid.
Akesson-Nilsson
Gunilla
G
Department of Chemistry and Biomedical Sciences, University of Kalmar, SE-391 82 Kalmar, Sweden. gunilla.akesson-nilsson@hik.se
D000818:Animals; D002460:Cell Line; D017606:Chlorine Compounds / Q000302:isolation & purification*; D002849:Chromatography, Gas / Q000379:methods*; D017077:Culture Media, Conditioned / Q000737:chemistry*; D004524:Eels; D004951:Esterification; D004952:Esters / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D008745:Methylation
D017606:Chlorine Compounds; D017077:Culture Media, Conditioned; D004952:Esters; D005227:Fatty Acids; D008055:Lipids