Crystallization and preliminary X-ray diffraction analysis of lectin-1 from Pseudomonas aeruginosa.
Carbohydrate recognition plays a role in the pathogenesis of Pseudomonas aeruginosa, a common cause of opportunistic infection in humans. Crystals of a carbohydrate-binding protein from P. aeruginosa, lectin PA-1, have now been obtained. The crystals belong to space group I222, with unit-cell parameters a = 40.25, b = 72.30, c = 133.82 A, and diffract to beyond 1.9 A resolution on a rotating-anode X-ray source. Details of crystal-growth conditions, diffraction data collection and processing are reported.
Karaveg
Khanita
K
Department of Microbiology and Immunology, University of Louisville, Louisville, KY 40292, USA.
Liu
Zhi Jie
ZJ
Tempel
Wolfram
W
Doyle
Ronald J
RJ
Rose
John P
JP
Wang
Bi Cheng
BC
D003460:Crystallization / Q000379:methods; D037161:Galectins / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000737:chemistry*; D014961:X-Ray Diffraction / Q000379:methods
D037161:Galectins