[Development of a new biosensor for biochemical oxygen demand].
OBJECTIVE
To use a new kind of fixing material, i.e. Sol-Gel organic-inorganic hybridized material to immobilize bacterium to detect Biochemical oxygen demand quickly.


METHODS
The biosensor was fabricated using a thin film in which Hansenula anomala was immobilized by sol-gel and an oxygen electrode. The optimum measurement for biochemical oxygen demand was at pH 7.0; 28 degrees C; response time 3 - 12 min. Pure organic compound, sewage and rate of recovery were detected with the biosensor.


RESULTS
It shows that the BOD biosensor can be used to detect many organic compounds such as amino acid, glucide. It is suitable to monitor sewage and industrial waste water which has low level alcohols and phenols. The microbial membrane can work 3 months and remain its 70% activity. It is measured that the rate of recovery of BOD is between 90% to 105% in sewage.


CONCLUSIONS
The study confirmed the effectiveness and usefulness of BOD sensor, which is quick, convenient, low cost and reliable with little interference.
Chang
Dong
D
The First Hospital of Harbin Medical University, Harbin 150001, China.
Du
Xiao-yan
XY
Pan
Hong-zhi
HZ
Jia
Jian-bo
JB
Wang
Bing-quan
BQ
Cheng
Guang-jin
GJ
Dong
Shao-jun
SJ
D001419:Bacteria; D015374:Biosensing Techniques / Q000295:instrumentation*; D018914:Cells, Immobilized; D005782:Gels; D008567:Membranes, Artificial; D009757:Nylons; D010100:Oxygen / Q000032:analysis*; D012722:Sewage / Q000032:analysis / Q000382:microbiology
D005782:Gels; D008567:Membranes, Artificial; D009757:Nylons; D012722:Sewage; D010100:Oxygen