title: Antigen feeding increases frequency and antigen-specific proliferation ability of intraepithelial CD4+ T cells in alphabeta T cell receptor transgenic mice.
abstract: To study how intestinal intraepithelial lymphocytes (IEL) are affected by orally ingested antigen, the phenotypes and responses of the IEL in mice expressing a transgenic T cell receptor alphabeta (TCR alphabeta) specific for ovalbumin (OVA) were analyzed after feeding OVA. In the OVA-fed mice, the abundance of alphabeta-IEL as a proportion of the total IEL population increased and the frequency of CD4+ cells increased within the TCR alphabeta+ IEL population. CD4(+) IEL from OVA-fed transgenic mice proliferated in vitro more markedly in response to antigen stimulation than IEL from mice fed the control diet. These results indicate that antigen-specific proliferation of CD4+ IEL was amplified as a result of oral administration of antigen.
authors.affiliation: Department of Applied Biological Chemistry, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Masao
authors.initials: M
authors.lastname: Goto
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hachimura
authors.forename: Akio
authors.initials: A
authors.lastname: Ametani
authors.forename: Takehito
authors.initials: T
authors.lastname: Sato
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kumagai
authors.forename: Sonoko
authors.initials: S
authors.lastname: Habu
authors.forename: Mamoru
authors.initials: M
authors.lastname: Totsuka
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Ishikawa
authors.forename: Shuichi
authors.initials: S
authors.lastname: Kaminogawa
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000941:Antigens / Q000008:administration & dosage* / Q000276:immunology / Q000494:pharmacology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002455:Cell Division / Q000276:immunology; D002478:Cells, Cultured; D016130:Immunophenotyping; D007413:Intestinal Mucosa; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D008822:Mice, Transgenic; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics / Q000276:immunology*
chemical_list: D000941:Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta; D010047:Ovalbumin