Twelve-week RBC storage.
BACKGROUND
Better storage can improve RBC availability and safety. Optimizing RBC ATP production and minimizing hemolysis has allowed progressively longer storage.


METHODS
In the first study, 24 units of packed CPD RBCs were pooled in groups of four, realiquoted, and added to 300 mL of one of four variants of experimental additive solution 76 (EAS-76) containing 45, 40, 35, or 30 mEq per L NaCl. Units were sampled weekly for 12 weeks for morphologic and biochemical measures. In the second study, 10 volunteers donated 2 units of RBCs for a crossover comparison of Tc/Cr 24-hour in vivo recovery of 6-week storage in AS-1 versus 12-week storage in EAS-76 variant 6 (EAS-76v6) having 30 mEq per L NaCl.


RESULTS
RBCs stored in the lower salt variants of EAS-76 had higher concentrations of RBC ATP with less hemolysis and microvesiculation. RBC 2,3 DPG was preserved for two weeks. RBCs stored for 12 weeks in EAS-76v6 exhibited 78 +/- 4 percent 24-hour in vivo recovery.


CONCLUSIONS
It is possible to store RBCs for 12 weeks with acceptable recovery and 0.6 percent hemolysis and with normal 2,3 DPG concentrations for 2 weeks.
Hess
John R
JR
Blood Research Detachment, Walter Reed Army Institute of Research, Washington, DC, USA. jhess@umm.edu
Hill
Heather R
HR
Oliver
Cindy K
CK
Lippert
Lloyd E
LE
Rugg
Neeta
N
Joines
Amy D
AD
Gormas
Jennifer F
JF
Pratt
P Gayle
PG
Silverstein
Edward B
EB
Greenwalt
Tibor J
TJ
D019794:2,3-Diphosphoglycerate / Q000097:blood; D000255:Adenosine Triphosphate / Q000097:blood; D001782:Blood Donors; D001793:Blood Preservation / Q000379:methods; D017707:Erythrocyte Transfusion; D004912:Erythrocytes; D005260:Female; D006461:Hemolysis; D006801:Humans; D008297:Male; D012965:Sodium Chloride / Q000032:analysis; D012996:Solutions; D013997:Time Factors
D012996:Solutions; D019794:2,3-Diphosphoglycerate; D012965:Sodium Chloride; D000255:Adenosine Triphosphate