Identification of a glucocorticoid response element in the 3'-flanking region of the human Dexras1 gene.
The Dexras1 gene responds to glucocorticoids with a rapid and profound induction. A glucocorticoid response element (GRE) was identified in the 3'-flanking region (2.3 kb downstream of poly(A) signal) of the human Dexras1 gene. This element conferred rapid glucocorticoid responsiveness when inserted into a homologous promoter-driven luciferase reporter. A point mutation within the 15-bp GRE abolished this glucocorticoid responsiveness.
Kemppainen
Robert J
RJ
Department of Anatomy, Physiology and Pharmacology, Auburn University, College of Veterinary Medicine, 213 Greene Hall, Auburn, AL 36849, USA. kempprj@vetmed.auburn.edu
Cox
Elaine
E
Behrend
Ellen N
EN
Brogan
Michele D
MD
Ammons
Jessica M
JM
D024509:3' Flanking Region; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003907:Dexamethasone / Q000494:pharmacology; D019204:GTP-Binding Proteins; D005938:Glucocorticoids / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D020559:Monomeric GTP-Binding Proteins / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D017354:Point Mutation; D011061:Poly A; D011401:Promoter Regions, Genetic; D020218:Response Elements; D018631:ras Proteins
D005938:Glucocorticoids; C400132:RASD1 protein, human; D011061:Poly A; D003907:Dexamethasone; D008156:Luciferases; D019204:GTP-Binding Proteins; D020559:Monomeric GTP-Binding Proteins; D018631:ras Proteins