title: Functional genomics guided with MR imaging: mouse tumor model study.
abstract: To gain a better understanding of gene expression patterns in tumors, the authors used contrast material-enhanced magnetic resonance (MR) imaging to noninvasively characterize regions within the same tumor to provide a correlate for genomic analysis. Gene expression profiles of samples from a mouse tumor model obtained from contrast-enhanced and nonenhanced regions within the same tumor were compared with MR imaging and functional genomics. From these samples, 11000 genes were analyzed: 10 genes were up-regulated in the contrast-enhanced areas, and one gene was up-regulated in the nonenhanced regions. Several of these genes encode extracellular matrix proteins. Findings in this study demonstrate that MR imaging can serve as a powerful noninvasive tool for characterizing different regions of tumors to guide genomic analysis with high spatial and temporal resolution.
authors.affiliation: Department of Radiology, Stanford University School of Medicine, 1201 Welch Rd, P260, Stanford, CA 94305-5488, USA.
authors.forename: Samira
authors.initials: S
authors.lastname: Guccione
authors.forename: Yi-Shan
authors.initials: YS
authors.lastname: Yang
authors.forename: Gongyi
authors.initials: G
authors.lastname: Shi
authors.forename: Daniel Y
authors.initials: DY
authors.lastname: Lee
authors.forename: King C P
authors.initials: KC
authors.lastname: Li
authors.forename: Mark D
authors.initials: MD
authors.lastname: Bednarski
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D003287:Contrast Media; D018450:Disease Progression; D019786:Gadolinium DTPA; D020869:Gene Expression Profiling; D023281:Genomics; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D003287:Contrast Media; D019786:Gadolinium DTPA