title: Linking peanut allergenicity to the processes of maturation, curing, and roasting.
abstract: The processes of peanut maturation, curing, and roasting are known to have an important role in peanut flavors. One of these processes (i.e., roasting) has been found to have an effect on allergenicity. To determine if the other processes (i.e., maturation and curing) affect allergenicity, mature and immature roasted peanuts and peanuts cured at different temperatures (35-77 degrees C) were, respectively, tested for IgE binding and advanced glycation end adducts (AGEs). Peanuts with and without stress proteins, which are associated with peanut maturation and curing, were also tested. Results showed that mature roasted peanuts exhibited a higher IgE binding and AGEs level than immature roasted peanuts. Curing temperatures between 35 and 60 degrees C gave no difference in the profiles. However, a higher curing temperature (i.e., 77 degrees C) exhibited a profile of higher levels of AGEs and IgE binding. These levels were higher in peanuts with stress proteins than without stress proteins. Roasting increased stress protein level and IgE binding. From these results, the processes of maturation and curing, in conjunction with roasting, may be associated with allergenicity, suggesting that these processes may lead to changes in the allergenic properties of peanuts.
authors.affiliation: Southern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, 1100 Robert E. Lee Boulevard, New Orleans, Louisiana 70124, USA. sychung@srrc.ars.usda.gov
authors.forename: Si-Yin
authors.initials: SY
authors.lastname: Chung
authors.forename: Christopher L
authors.initials: CL
authors.lastname: Butts
authors.forename: Soheila J
authors.initials: SJ
authors.lastname: Maleki
authors.forename: Elaine T
authors.initials: ET
authors.lastname: Champagne
mesh_terms: D000485:Allergens / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D010367:Arachis / Q000737:chemistry / Q000254:growth & development / Q000276:immunology*; D005511:Food Handling; D017127:Glycation End Products, Advanced / Q000032:analysis; D006358:Hot Temperature; D007073:Immunoglobulin E / Q000378:metabolism; D021183:Peanut Hypersensitivity / Q000276:immunology*; D010936:Plant Extracts / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D010940:Plant Proteins / Q000032:analysis; D012639:Seeds / Q000737:chemistry / Q000254:growth & development / Q000276:immunology; D013997:Time Factors
chemical_list: D000485:Allergens; D017127:Glycation End Products, Advanced; D010936:Plant Extracts; D010940:Plant Proteins; C070556:dehydrin proteins, plant; D007073:Immunoglobulin E