title: The effect of intraperitoneally administered recombinant IL-1beta on immune parameters and resistance to Aeromonas salmonicida in the rainbow trout (Oncorhynchus mykiss).
abstract: The present work provides the first information concerning the immunostimulatory activity of trout recombinant interleukin-1 beta (rIL-1beta) in vivo. The predicted rainbow trout mature IL-1beta peptide was produced as a recombinant protein in Escherichia coli. Optimal migration of peritoneal leucocytes and rIL-1beta induced phagocytosis occurred following intraperitoneal injection of 1 microg of the recombinant protein. Moreover, systemic IL-1beta, COX-2 and lysozyme II gene expression was enhanced following >or=1 microg rIL-1beta administration. Finally, resistance to Aeromonas salmonicida, the causative agent of furunculosis, was augmented at early times (2 days) post-injection of 1 microg rIL-1beta.
authors.affiliation: Department of Zoology, University of Aberdeen, Tillydrone Avenue, AB24 2TZ, Scotland, Aberdeen, UK.
authors.forename: Suhee
authors.initials: S
authors.lastname: Hong
authors.forename: Scott
authors.initials: S
authors.lastname: Peddie
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Campos-Pérez
authors.forename: Jun
authors.initials: J
authors.lastname: Zou
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Secombes
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000333:Aeromonas / Q000276:immunology*; D000818:Animals; D051546:Cyclooxygenase 2; D004305:Dose-Response Relationship, Drug; D016905:Gram-Negative Bacterial Infections / Q000276:immunology*; D007107:Immune System / Q000187:drug effects*; D007375:Interleukin-1 / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology*; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D007958:Leukocyte Count; D007962:Leukocytes / Q000187:drug effects; D009113:Muramidase / Q000096:biosynthesis / Q000235:genetics; D017686:Oncorhynchus mykiss / Q000276:immunology* / Q000382:microbiology; D010587:Phagocytosis / Q000187:drug effects; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis / Q000235:genetics
chemical_list: D000276:Adjuvants, Immunologic; D007375:Interleukin-1; D007527:Isoenzymes; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases; D009113:Muramidase