title: Expression of tumor markers on breast and ovarian cancer cell lines.
abstract: BACKGROUND
  Breast and ovarian tumor cell lines with defined tumor-associated antigens could serve as allogeneic antigen-sources for the biological therapy of cancer.
  
  
  METHODS
  Eight breast and 3 ovarian cancer cell lines were stained for the antigens in question by immunohistochemistry. Antigen-expression was graded in 5 steps by light microscopic examination.
  
  
  RESULTS
  All cell lines with the exception of PA-1 expressed EMA. Only MCF-7 was CEA-positive. MUC-1 was found in all cell lines apart from MDA-MB and SK-OV3. The highest expression of Her-2/neu was found in ZR-75/1 and SK-OV3. A491D and PA1 cells were CD19-negative, all other lines were positive. Only A491D and BT-20 had p53 accumulation. PR was found in 5 cell lines and ER in 6 cell lines.
  
  
  CONCLUSIONS
  The cell lines investigated express a different pattern of tumor markers. In consequence, a careful examination should be performed in order to find the "best fitting" cell line to a patient's tumor for a tumor-vaccine.
authors.affiliation: Department of Obstetrics and Gynecology, University of Wuerzburg, Germany. frak057@mail.uni-wuerzburg.de
authors.forename: Ulrike
authors.initials: U
authors.lastname: Kämmerer
authors.forename: Florian
authors.initials: F
authors.lastname: Thanner
authors.forename: Michaela
authors.initials: M
authors.lastname: Kapp
authors.forename: Johannes
authors.initials: J
authors.lastname: Dietl
authors.forename: Marc
authors.initials: M
authors.lastname: Sütterlin
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D001943:Breast Neoplasms / Q000737:chemistry* / Q000145:classification / Q000473:pathology; D002272:Carcinoembryonic Antigen / Q000032:analysis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D010051:Ovarian Neoplasms / Q000737:chemistry* / Q000145:classification / Q000473:pathology; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis; D014407:Tumor Cells, Cultured; D014746:Vimentin / Q000032:analysis
chemical_list: D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D018719:Erb-b2 Receptor Tyrosine Kinases; D014746:Vimentin