Completed sequence of plasmid pIP501 and origin of spontaneous deletion derivatives.
The sequence of plasmid pIP501 (30,603 bp) was completed using previously published and newly acquired data. The sites at which two spontaneous deletions had occurred were identified. One was between tracts of repeated heptamers and the other between regions of secondary structure associated with plasmid replication. A high level of identity ( >95%) between plasmid pIP501 and part of plasmid pRE25, which had been isolated from Enterococcus faecalis associated with a food source, was confirmed.
Thompson
J K
JK
Department of Food Science (Food Microbiology), Department of Agriculture and Rural Development for Northern Ireland, Newforge Lane, Belfast BT9 5PX, Northern Ireland, UK. keith.thompson@dardni.gov.uk
Collins
M A
MA
D001483:Base Sequence; D013293:Enterococcus faecalis / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion / Q000235:genetics*