Effect of ligustrazine on expression of adherent molecule CD49d and cyclin D2 in hematopoietic cells in acute radiation injured mice.
After irradiation by 8.0 Gy gamma-ray, each mouse was stomach-fed by 4 mg ligustrazine injection twice a day. On the 7th day after irradiation, CD49d expression in ligustrazine-treated group was significantly higher than that in control group (P < 0.01), and showed no difference from that in normal group (P > 0.05). On the 14th day after irradiation. CD49d expression was increased in control group, but decreased significantly in ligustrazine-treated group (P < 0.01). The expression of Cyclin D2 in spleen mononuclear cells (MNC) in ligustrazine-treated group was significantly higher than that in control group, but the ratio of G0 + G1 phase cells was significantly lower in ligustrazine-treated group (P < 0.01). This finding indicated that ligustrazine could increase the expression of adherent molecule on bone marrow hematopoietic cells and Cyclin D2 in spleen MNC, thereby promoting the growth of hematopoietic cells.
Dong
L
L
Department of Hematology, Tongji Hospital, Tongji Medical University, Wuhan 430030.
Sun
H
H
Liu
W
W
Tao
D
D
Zhang
Y
Y
Xu
H
H
D000818:Animals; D056742:Cyclin D2; D016213:Cyclins / Q000096:biosynthesis*; D005260:Female; D005720:Gamma Rays; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D039441:Integrin alpha4 / Q000096:biosynthesis*; D051379:Mice; D011719:Pyrazines / Q000494:pharmacology*; D011832:Radiation Injuries / Q000378:metabolism*; D011897:Random Allocation; D013154:Spleen / Q000166:cytology; D014916:Whole-Body Irradiation
C486196:Ccnd2 protein, mouse; D056742:Cyclin D2; D016213:Cyclins; D011719:Pyrazines; D039441:Integrin alpha4; C017953:tetramethylpyrazine