Efficient cross-linking to cytidine under neutral conditions using the 2-amino-6-vinylpurine derivative having an additional activating group.
We have previously reported that the oligonucleotides incorporating 2-amino-6-vinyl purine analog (1) showed efficient interstrand cross-linking with selectivity toward cytidine at a target site. In this paper, we describe the design of more efficient cross-linking reagent (2) bearing 2-amino-6-vinyl purine motif to react to cytidine selectively under neutral condition.
Nagatsugi
F
F
Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Tokuda
N
N
Kawasaki
T
T
Maeda
M
M
Sasaki
S
S
D001483:Base Sequence; D003432:Cross-Linking Reagents / Q000138:chemical synthesis* / Q000737:chemistry*; D003562:Cytidine / Q000737:chemistry*; D007700:Kinetics; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D011687:Purines / Q000737:chemistry*; D014753:Vinyl Compounds / Q000737:chemistry*
C432203:2-amino-6-vinylpurine; D003432:Cross-Linking Reagents; D009841:Oligonucleotides; D011687:Purines; D014753:Vinyl Compounds; D003562:Cytidine