Effect of total or partial uterus extirpation on uterus-projecting neurons in porcine inferior mesenteric ganglion. C. Changes in expression of apoptosis-associated (Bcl-2 and Bax) and regeneration-associated (GAP-43) proteins.
The expression of Bcl-2 and Bax proteins was studied with immunohistochemistry, immuoblotting and RT-PCR in the uterine horn- and uterine cervix-projecting neurons of the inferior mesenteric ganglion (IMG) of the sexually immature gilts after partial or total hysterectomy. Additionally, the expression of regeneration-associated protein GAP-43 was studied in these neurons with immunohistochemistry. The uterus-projecting neurons were identified with retrograde fluorescent tracer Fast Blue (FB). The weak immunoreactivity to Bcl-2 and GAP-43 and moderately intense immunoreactivity to Bax was revealed in all FB+ (FB+) neurons of control and hysterectomized pigs. No difference in the intensity of immunostaining for Bcl-2, Bax and GAP-43 was found between control and hysterectomized gilts. Immunoblotting revealed the presence of Bcl-2 and Bax proteins in IMGs of control and hysterectomized animals and no difference in the band intensities between control and experimental groups was detected. RT-PCR detected weak induction of bcl-2 and bax only in the ganglia of animals which had undergone total hysterectomy. It was found that the axotomy of the uterus-projecting neurons located in the porcine IMG did not change the expression of the studied substances (Bcl-2, Bax and GAP-43) at protein level and only the induction of bcl-2 and bax at the level of RNA was visible.
Wasowicz
K
K
University of Warmia and Mazury, Department of Animal Anatomy, Oczapowskiego 13, 10-957 Olsztyn, Poland. krzysztof.wasowicz@uwm.edu.pl
D000818:Animals; D017931:DNA Primers; D005260:Female; D019922:GAP-43 Protein / Q000378:metabolism; D005728:Ganglia, Sympathetic / Q000378:metabolism*; D015151:Immunoblotting / Q000662:veterinary; D007150:Immunohistochemistry / Q000662:veterinary; D008643:Mesentery / Q000294:innervation; D009474:Neurons / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine; D014599:Uterus / Q000294:innervation* / Q000601:surgery; D051028:bcl-2-Associated X Protein
D017931:DNA Primers; D019922:GAP-43 Protein; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein