ELISA detection of clonazepam and 7-aminoclonazepam in whole blood and urine.
The ability of five commercially available enzyme-linked immunosorbent assay (ELISA) benzodiazepines to detect clonazepam and 7-aminoclonazepam in blood and urine was investigated. To determine the cross-reactivity of various ELISA assays, drug free blood and urine were fortified with clonazepam and 7-aminoflunitrazepam at concentrations of 1, 2.5, 5, 10, and 25microg/dl. The cross-reactivity, with respect to oxazepam, for clonazepam was 16, 37, 80, 93, and 109% with Immunalysis, Diagnostix, Neogen, OraSure, and Cozart, respectively; for 7-aminoclonazepam, none of the five ELISA assays showed any cross-reactivity above 10%.
Elian
Albert A
AA
Forensic Toxicology Unit, State Police Crime Laboratory, Commonwealth of Massachusetts, 59 Horsepond Road, Sudbury, MA 01776, USA.
D014151:Anti-Anxiety Agents / Q000097:blood / Q000652:urine; D000927:Anticonvulsants / Q000097:blood* / Q000652:urine*; D002998:Clonazepam / Q000031:analogs & derivatives* / Q000097:blood* / Q000652:urine*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D010076:Oxazepam / Q000097:blood / Q000652:urine; D012015:Reference Standards
D014151:Anti-Anxiety Agents; D000927:Anticonvulsants; C020860:7-aminoclonazepam; D002998:Clonazepam; D010076:Oxazepam