title: Improving quality and harvest period of protein crystals for structure-based drug design: effects of a gel and a magnetic field on bovine adenosine deaminase crystals.
abstract: The overall crystal quality as well as the harvest period of bovine adenosine deaminase containing a zinc ion at the active centre has been compared in three different environments: crystallization as a control, crystallization with agarose gel and crystallization in a high magnetic field. In crystallization with agarose gel, the probability of obtaining high-quality crystals was somewhat increased, but the harvest period was elongated. On the other hand, in crystallization in a magnetic field, the probability of obtaining high-quality crystals was greatly increased. Furthermore, the harvest period for crystal growth in a magnetic field was much shorter than that with agarose gel.
authors.affiliation: Exploratory Research Laboratories, Fujisawa Pharmaceutical Co Ltd, 5-2-3 Tokodai, Tsukuba, Ibaraki 300-2698, Japan. takayoshi_kinoshita@po.fujisawa.co.jp
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Kinoshita
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Ataka
authors.forename: Masaichi
authors.initials: M
authors.lastname: Warizaya
authors.forename: Masahiro
authors.initials: M
authors.lastname: Neya
authors.forename: Takashi
authors.initials: T
authors.lastname: Fujii
mesh_terms: D000243:Adenosine Deaminase / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D003460:Crystallization / Q000379:methods / Q000592:standards*; D018360:Crystallography, X-Ray; D015195:Drug Design; D005782:Gels; D008280:Magnetics; D011506:Proteins / Q000737:chemistry; D012685:Sepharose
chemical_list: D005782:Gels; D011506:Proteins; D012685:Sepharose; D000243:Adenosine Deaminase