title: The effect of under- and overexpressed CoEXG1-encoded exoglucanase secreted by Candida oleophila on the biocontrol of Penicillium digitatum.
abstract: The yeast, Candida oleophila, is acknowledged for its biocontrol activity against postharvest moulds. However, the mechanism of this activity is not fully understood. One of the conjectured modes of action is associated with extracellular lytic enzymes, such as beta-exoglucanase. The relationship of beta-exoglucanase in the biocontrol activity of C. oleophila was investigated by generating C. oleophila CoEXG1-knockouts and double-CoEXG1 transformants. The knockout transformants secreted 0-13% of the exoglucanase activity detected in the medium of the untransformed yeast (depending on the medium), indicating that CoEXG1 is the main gene responsible for the production of the secreted exoglucanase. Correspondingly, the double-CoEXG1 transformants secreted approximately twice as much 1,3-beta-exoglucanase as the untransformed C. oleophila. The biocontrol activity of the CoEXG1-knockout and the double-CoEXG1 transformants against Penicillium digitatum did not differ from that of the untransformed C. oleophila on kumquats. These results imply that the 1,3-beta-exoglucanase encoded by the gene CoEXG1 is not involved in the biocontrol activity of C. oleophila against P. digitatum under these experimental terms. However, these findings do not rule out the possibilities, that the participation of CoEXG1 in biocontrol is dependent on the activity of other gene products, or that its effect may be manifested under altered environmental conditions.
authors.affiliation: Department of Molecular Genetics, MIGAL Galilee Technology Center, PO Box 831, Kiryat-Shmona 11016, Israel.
authors.forename: Hila
authors.initials: H
authors.lastname: Yehuda
authors.forename: Samir
authors.initials: S
authors.lastname: Droby
authors.forename: Meirav
authors.initials: M
authors.lastname: Bar-Shimon
authors.forename: Michael
authors.initials: M
authors.lastname: Wisniewski
authors.forename: Martin
authors.initials: M
authors.lastname: Goldway
mesh_terms: D015139:Blotting, Southern; D002175:Candida / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D043326:Glucan 1,3-beta-Glucosidase; D010407:Penicillium / Q000254:growth & development* / Q000378:metabolism; D010572:Pest Control, Biological / Q000379:methods*; D016133:Polymerase Chain Reaction; D013172:Spores, Fungal / Q000254:growth & development; D014170:Transformation, Genetic; D001617:beta-Glucosidase / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D004271:DNA, Fungal; D001617:beta-Glucosidase; D043326:Glucan 1,3-beta-Glucosidase