title: Synthesis of probes with broad pH range fluorescence.
abstract: Reaction of a rhodamine 2'-ester with an excess of alkyldiamines provides amino-functionalized rhodamine spirolactams, which when subsequently conjugated with carboxyfluorescein, provides probes which are fluorescent at acidic, neutral, and basic pH ranges.
authors.affiliation: Department of Chemistry (D9MD), Abbott Diagnostics Division, Abbott Laboratories, 100 Abbott Park Road, 60064-6016, Abbott Park, IL, USA. maciej.adamczyk@abbott.com
authors.forename: Maciej
authors.initials: M
authors.lastname: Adamczyk
authors.forename: Jonathan
authors.initials: J
authors.lastname: Grote
mesh_terms: D002463:Cell Membrane Permeability; D002851:Chromatography, High Pressure Liquid; D005452:Fluoresceins / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007769:Lactams / Q000138:chemical synthesis / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D012235:Rhodamines / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D007769:Lactams; D012235:Rhodamines; C024098:6-carboxyfluorescein