Expansion of the glycosynthase repertoire to produce defined manno-oligosaccharides.
Mutant endo-mannanases, in which the catalytic nucleophile has been replaced, function as glycosynthases in the synthesis of manno-oligosaccharides of defined lengths.
Jahn
Michael
M
Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, BC V6T 1Z1, Canada.
Stoll
Dominik
D
Warren
R Antony J
RA
Szabó
Lóránd
L
Singh
Pritpal
P
Gilbert
Harry J
HJ
Ducros
Valérie M A
VM
Davies
Gideon J
GJ
Withers
Stephen G
SG
D002240:Carbohydrate Sequence; D002384:Catalysis; D044602:Cellulases / Q000737:chemistry; D006020:Glycopeptides / Q000096:biosynthesis* / Q000737:chemistry; D006026:Glycoside Hydrolases / Q000737:chemistry*; D006031:Glycosylation; D006863:Hydrogen-Ion Concentration; D008351:Mannans / Q000737:chemistry; D008361:Mannosidases / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000737:chemistry*; D016297:Mutagenesis, Site-Directed; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000737:chemistry; D013997:Time Factors; D014166:Transferases / Q000737:chemistry*
D006020:Glycopeptides; D008351:Mannans; D009097:Multienzyme Complexes; D009844:Oligosaccharides; C023176:transglycosidase enzyme system; C014647:mannobiose; D014166:Transferases; D044602:Cellulases; D006026:Glycoside Hydrolases; D008361:Mannosidases; C119276:endo-beta-mannosidase