Flip the tip: an automated, high quality, cost-effective patch clamp screen.
The race for creating an automated patch clamp has begun. Here, we present a novel technology to produce true gigaseals and whole cell preparations at a high rate. Suspended cells are flushed toward the tip of glass micropipettes. Seal, whole-cell break-in, and pipette/liquid handling are fully automated. Extremely stable seals and access resistance guarantee high recording quality. Data obtained from different cell types sealed inside pipettes show long-term stability, voltage clamp and seal quality, as well as block by compounds in the pM range. A flexible array of independent electrode positions minimizes consumables consumption at maximal throughput. Pulled micropipettes guarantee a proven gigaseal substrate with ultra clean and smooth surface at low cost.
Lepple-Wienhues
Albrecht
A
Flyion GmbH, Tübingen, Waldhäuserstr. 64, D-72076, Tübingen, Germany. info@flyion.com
Ferlinz
Klaus
K
Seeger
Achim
A
Schäfer
Arvid
A
D000818:Animals; D001331:Automation; D001671:Biochemistry / Q000379:methods; D016466:CHO Cells; D002460:Cell Line; D006224:Cricetinae; D003600:Cytosol / Q000378:metabolism; D004566:Electrodes; D004594:Electrophysiology; D006801:Humans; D007473:Ion Channels; D008422:Materials Testing; D018408:Patch-Clamp Techniques / Q000295:instrumentation* / Q000379:methods*; D015221:Potassium Channels / Q000378:metabolism
D007473:Ion Channels; D015221:Potassium Channels