Discovery of imprinted transcripts in the mouse transcriptome using large-scale expression profiling.
Candidate imprinted transcriptional units in the mouse genome were identified systematically from 27,663 FANTOM2 full-length mouse cDNA clones by expression profiling. Large-scale cDNA microarrays were used to detect differential expression dependent upon chromosomal parent of origin by comparing the mRNA levels in the total tissue of 9.5 dpc parthenogenote and androgenote mouse embryos. Of the FANTOM2 transcripts, 2114 were identified as candidates on the basis of the array data. Of these, 39 mapped to known imprinted regions of the mouse genome, 56 were considered as nonprotein-coding RNAs, and 159 were natural antisense transcripts. The imprinted expression of two transcripts located in the mouse chromosomal region syntenic to the human Prader-Willi syndrome region was confirmed experimentally. We further mapped all candidate imprinted transcripts to the mouse and human genome and were shown in correlation with the imprinting disease loci. These data provide a major resource for understanding the role of imprinting in mammalian inherited traits.
Nikaido
Itoshi
I
Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center (GSC), RIKEN Yokohama Institute, Suehiro-cho, Tsurumi-ku Yokohama, Kanagawa 230-0045, Japan.
Saito
Chika
C
Mizuno
Yosuke
Y
Meguro
Makiko
M
Bono
Hidemasa
H
Kadomura
Moritoshi
M
Kono
Tomohiro
T
Morris
Gerard A
GA
Lyons
Paul A
PA
Oshimura
Mitsuo
M
Hayashizaki
Yoshihide
Y
Okazaki
Yasushi
Y
D000818:Animals; D002874:Chromosome Mapping / Q000379:methods; D030541:Databases, Genetic; D004622:Embryo, Mammalian; D020869:Gene Expression Profiling / Q000379:methods*; D005819:Genetic Markers / Q000235:genetics; D018392:Genomic Imprinting / Q000235:genetics*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011218:Prader-Willi Syndrome / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D022661:RNA, Untranslated / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic / Q000235:genetics*
D005819:Genetic Markers; D012333:RNA, Messenger; D022661:RNA, Untranslated