Changes in the peripheral leukocyte phenotype of calves in clinical cases of bronchopneumonia complicated with chlamydial co-infectious agent.
This paper reports the alterations in peripheral blood leukocyte phenotype in respiratory diseased calves affected with chlamydial and non-chlamydial co-infectious agent. The etiological contribution of chlamydial infectious agent in examined clinical cases of enzootic bronchopneumoniae syndrome was confirmed in affected calves serologically both by complement fixation test (CF) and enzyme immunoassay (EIA). Changes in leukocyte subpopulations in the blood of the calves were detected both with routine haematological methods and by FCM using specific monoclonal antibodies directed against CD14, CD45, CD2, CD4, CD8 and WC4 (a specific surface marker for bovine B-lymphocytes). The results obtained by flow cytometry analysis indicate that polymorfonuclear neutrophils (PMNLs) and T lymphocytes, especially CD8-positive cells, may play a significant role in cellular immune response against Chlamydophila psittaci (Chl. psittaci) co-infection in calves suffering from enzootic bronchopneumonia syndrome. A repercussion of this was a significant increase of the cell numbers in peripheral blood of the infected animals. Effective recruitment from a reserve marginal pool of these cells into blood vessels and activation of bone marrow proliferation are probably the reason for their high circulating number.
Niemczuk
K
K
Department of Cattle and Sheep Diseases, National Veterinary Research Institute, Al. Partyzantów 57, 24-100 Puławy, Poland.
Bednarek
D
D
D000818:Animals; D000831:Animals, Newborn; D001996:Bronchopneumonia / Q000150:complications / Q000276:immunology / Q000662:veterinary*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D023521:Chlamydophila Infections / Q000150:complications / Q000276:immunology / Q000662:veterinary*; D002691:Chlamydophila psittaci; D003168:Complement Fixation Tests / Q000662:veterinary; D005434:Flow Cytometry / Q000662:veterinary; D007124:Immunoenzyme Techniques / Q000662:veterinary; D007962:Leukocytes / Q000276:immunology*; D010641:Phenotype; D016176:T-Lymphocyte Subsets / Q000276:immunology