Evidence for a Schiff base formation of peptides derived from RecA with single-stranded oligonucleotides containing 5-formyl-2'-deoxyuridine.
We report some evidence for a Schiff base formation of 5-formyl-2'-deoxyuridine containing oligonucleotides with oligopeptide derived from the DNA binding site of RecA protein. We have demonstrated that cross-linked products were successfully detected by lowering the peptide concentrations. This is probably due to the reduced formation of large aggregates.
Sugiyama
T
T
Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Komaba, Meguro-ku, Tokyo 153-8902, Japan.
Kittaka
A
A
Takayama
H
H
Kuroda
R
R
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D003857:Deoxyuridine / Q000031:analogs & derivatives* / Q000737:chemistry*; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry* / Q000378:metabolism; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D017433:Protein Structure, Secondary; D011938:Rec A Recombinases / Q000737:chemistry* / Q000378:metabolism; D012545:Schiff Bases / Q000737:chemistry
C476360:FECO peptide; D009841:Oligonucleotides; D010455:Peptides; D012545:Schiff Bases; C061519:5-formyl-2'-deoxyuridine; D011938:Rec A Recombinases; D003857:Deoxyuridine