Hematologic recovery after autologous PBPC transplantation: importance of the number of postthaw CD34+ cells.
BACKGROUND
The implementation of a quality-assurance program is a major requirement to ensure quality and safety of the final PBPC components intended for clinical use. It is not clear whether the quantification of CFU-GM and CD34+ cells should be done on fresh components and after cryopreservation, which better represents the actual composition of the graft.


METHODS
Correlation between prefreeze and postthaw MNCs, CD34+ cells, and CFU-GM collected from 126 patients undergoing BMT (n=43) or PBPC (n =83) transplantation were evaluated. The statistical incidence of prefreeze and postthaw parameters as well as patient characteristics and conditioning regimens on hematologic recovery were analyzed.


RESULTS
By multivariate analysis, prefreeze and postthaw CD34+ cells were the only two variables significantly and independently correlated to hematologic recovery. Low prefreeze and postthaw CD34+ cell numbers associated to a low CD34+ yield characterize PBPC grafts from patients who have the slowest hematologic recovery. The postthaw PBPC CD34+ cell number can be estimated before conditioning regimen by thawing a small aliquot of the graft.


CONCLUSIONS
In association to prefreeze CD34+ cell number and to CD34+ yield, postthaw CD34+ cell number may be useful in monitoring cell loss during processing and identifying patients at risk of slow PBPC engraftment.
Feugier
Pierre
P
Department of Hematology, University Hospital, Nancy, France. p.feugier@chu-nancy.fr
Bensoussan
Daniele
D
Girard
Florence
F
Alla
Francois
F
Schuhmacher
Anne
A
Latger-Cannard
Veronique
V
Hulin
Cyrille
C
Witz
Francis
F
Witz
Brigitte
B
Carret
Anne Sophie
AS
Bordigoni
Pierre
P
Lederlin
Pierre
P
Janot
Christian
C
D000328:Adult; D018952:Antigens, CD34 / Q000032:analysis*; D001792:Blood Platelets / Q000166:cytology; D016026:Bone Marrow Transplantation; D002452:Cell Count; D015925:Cryopreservation; D005260:Female; D005434:Flow Cytometry; D006085:Graft Survival; D006098:Granulocytes / Q000166:cytology; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006358:Hot Temperature; D006801:Humans; D008297:Male; D036102:Peripheral Blood Stem Cell Transplantation; D017713:Platelet Transfusion; D014182:Transplantation, Autologous; D016896:Treatment Outcome
D018952:Antigens, CD34