title: Identification of suberization-associated anionic peroxidase as a possible allergenic protein from tomato.
abstract: A 45 kDa protein, which is recognized by IgE antibodies in sera of food-allergic patients, was purified and characterized as an allergenic protein from the tomato. The IgE-binding protein purified from tomato extract was found to be a glycoprotein with a molecular weight of approximately 45,000, an isoelectric point of 4.2, and no free N-terminal amino group. Furthermore, it was shown that the purified protein had peroxidase activity. From the amino acid sequence of a peptide fragment prepared by lysylendopeptidase digestion, the allergenic protein was identified to be the tomato suberization-associated anionic peroxidase 1 known as one of the pathogenesis-related proteins widely distributed in plants. These properties suggested the protein isolated from tomato to be a new allergenic protein in plant foodstuffs.
authors.affiliation: Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.
authors.forename: Thanakorn
authors.initials: T
authors.lastname: Weangsripanaval
authors.forename: Noriko
authors.initials: N
authors.lastname: Nomura
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Moriyama
authors.forename: Nobuo
authors.initials: N
authors.lastname: Ohta
authors.forename: Tadashi
authors.initials: T
authors.lastname: Ogawa
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000302:isolation & purification; D000595:Amino Acid Sequence; D002648:Child; D002675:Child, Preschool; D003876:Dermatitis, Atopic / Q000276:immunology; D005512:Food Hypersensitivity / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000276:immunology; D007223:Infant; D007518:Isoantibodies / Q000097:blood; D018551:Solanum lycopersicum / Q000276:immunology*; D010544:Peroxidases / Q000276:immunology* / Q000302:isolation & purification; D010936:Plant Extracts / Q000032:analysis
chemical_list: D000485:Allergens; D007518:Isoantibodies; D010936:Plant Extracts; D007073:Immunoglobulin E; D010544:Peroxidases; C082159:tomato anionic peroxidase 1