Single-molecule surface enhanced resonance Raman spectroscopy of the enhanced green fluorescent protein.
In the present contribution, we demonstrated that surface-enhanced resonance Raman scattering spectra from single green fluorescent proteins (GFPs) were obtained. The most important findings are the direct detection of the conversion between a deprotonated and a protonated form of the chromophore at the single-molecule level via the corresponding vibrational fingerprints, and the fact that the enhanced green fluorescent protein (EGFP) also shows a high surface enhanced resonance Raman scattering (SERRS) signal. Our findings show the potential of the technique to study structural dynamics of protein molecules at a single-molecule level.
Habuchi
Satoshi
S
Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200 F, 3001 Heverlee, Belgium.
Cotlet
Mircea
M
Gronheid
Roel
R
Dirix
Gunter
G
Michiels
Jan
J
Vanderleyden
Jos
J
De Schryver
Frans C
FC
Hofkens
Johan
J
D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins