[Inactivation of reporter genes by cloned heterochromatic repeats of Drosophila melanogaster is accompanied by chromatin compaction].
Cloned Stellate heterochromatic repeats caused unstable mosaic inactivation (position effect variegation; PEV) of the reporter gene mini-white. A number of known protein modifiers of the classical position effect induced by large heterochromatin blocks do not affect the expression of mini-white. This raises the question as to the specificity of chromatin compaction around the reporter gene. The inactivation of the mini-white gene has been found to be accompanied by a decrease in its methylation catalyzed by Escherichia coli dam-methyltransferase expressed in the genome of Drosophila. However, no changes in the nucleosome organization of mini-white have been found.
Naumova
N M
NM
Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182 Russia.
Olenkina
O M
OM
Gvozdev
V A
VA
D000818:Animals; D002843:Chromatin / Q000235:genetics / Q000378:metabolism*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D019175:DNA Methylation; D004331:Drosophila melanogaster / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D020868:Gene Silencing; D005798:Genes, Bacterial; D017344:Genes, Insect; D017930:Genes, Reporter; D006570:Heterochromatin / Q000235:genetics*; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid
D002843:Chromatin; D006570:Heterochromatin; D004247:DNA; D008780:Methyltransferases