[Contribution to the detection of Melan-A/Mart-1 specific CD8+ T cells in surgery of melanoma. Preliminary study].
Melanoma treatment is based on surgery. In metastatic cases, vaccine therapy has been recently developed to overcome T cell or dendritic cell dysfunction. HLA tetramerbased assays are useful for immunologic monitoring of this trial and to quantitate CD8+ specific lymphocytes. In the present work, we used tetrameric technology to detect expanded populations of tumor specific CD8+ Tcells specific of Melan-A/Mart-1 Antigen have been quantified using flow cytometry. The feasibility of routinely detection of 0,1% +/- 0,03 of CD8 T cells has been demonstrated, without any difference the levels observed before and after (day 30) surgery. The value of experimentation of these cells should be determined in clinic and particularly to analyze surgical practice.
Chevrier
S
S
Service de chirurgie plastique et reconstructrice, hôpital Sud, CHU de Rennes, 16, boulevard de Bulgarie, 35056 cedex, Rennes, France. stephane.chevrier@chu-rennes.fr <stephane.chevrier@chu-rennes.fr>
Drenou
B
B
Adamski
H
H
Aillet
S
S
Watier
E
E
D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm; D016827:CD8 Antigens / Q000276:immunology*; D005260:Female; D015789:HLA-A2 Antigen / Q000276:immunology*; D006801:Humans; D058965:MART-1 Antigen; D008297:Male; D008545:Melanoma / Q000276:immunology / Q000378:metabolism / Q000601:surgery; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism*; D012878:Skin Neoplasms / Q000276:immunology / Q000378:metabolism / Q000601:surgery
D000951:Antigens, Neoplasm; D016827:CD8 Antigens; D015789:HLA-A2 Antigen; D058965:MART-1 Antigen; C548667:MLANA protein, human; D009363:Neoplasm Proteins