[Analysis of PKC targeting mechanism using PKC fused with fluorescent proteins].
Protein kinase C (PKC) is a family, which consists of at least ten subtypes. To elucidate subtype-specific functions of PKC, we have developed the methods to observe PKC translocation in real time and in the living state using PKC fused with fluorescent proteins, including GFP and DsRed. Based on the live imaging of PKC translocation, we have demonstrated that PKC showed stimulus- and subtype-specific translocation, which can recognize its specific substrate and induce its specific cellular response (PKC targeting). These findings suggest that PKC targeting is the molecular basis underlying the diversity of PKC functions. Live imaging of PKC translocation has been proved to be a beneficial tool for understanding not only PKC functions, but also PKC-mediated signal transduction pathways. We have further analyzed PKC functions in the central nervous system using transgenic mice, which can express PKC-GFP in a brain-region-specific manner.
Sakai
Norio
N
Department of Molecular and Pharmacological Neuroscience, Graduate School of Biomedical Sciences, Hiroshima University, Japan. nsakai@hiroshima-u.ac.jp
Shirai
Yasuhito
Y
Saito
Naoaki
N
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000378:metabolism; D004075:Diglycerides / Q000502:physiology; D049452:Green Fluorescent Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008164:Luminescent Proteins; D008565:Membrane Proteins; D051379:Mice; D008822:Mice, Transgenic; D000076250:Myristoylated Alanine-Rich C Kinase Substrate; D010766:Phosphorylation; D011493:Protein Kinase C / Q000502:physiology*; D021381:Protein Transport; D011506:Proteins / Q000378:metabolism; D018048:Receptors, Purinergic P2 / Q000187:drug effects / Q000502:physiology; D015398:Signal Transduction
D004075:Diglycerides; D047908:Intracellular Signaling Peptides and Proteins; D008164:Luminescent Proteins; D008565:Membrane Proteins; D011506:Proteins; D018048:Receptors, Purinergic P2; D000076250:Myristoylated Alanine-Rich C Kinase Substrate; D049452:Green Fluorescent Proteins; D000255:Adenosine Triphosphate; D011493:Protein Kinase C