Shear-flow-based chromatographic separations as an alternative to pressure-driven liquid chromatography.
It is only by developing specially designed injection and detection systems that shear-driven chromatography can become a viable alternative to HPLC. In the present paper, a dedicated zero dead-volume injection procedure is presented with which sample volumes can be injected reproducibly in the required picoliter range. In addition, a transversal detection groove system is designed which should allow to perform on-line UV-VIS absorption measurements with path lengths in the millimeter range, with an acceptable theoretical plate loss (only 20% in a 5 cm long channel) and acting as a nearly perfect wave guide.
Desmet
G
G
Vrije Universiteit Brussel, Department of Chemical Engineering, Pleinlaan 2, 1050 Brussels, Belgium. gedesmet@vub.ac.be
Vervoort
N
N
Clicq
D
D
Huau
A
A
Gzil
P
P
Baron
G V
GV
D000465:Algorithms; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods; D004058:Diffusion; D013056:Spectrophotometry, Ultraviolet