Design and application of a new cryptic-plasmid-based shuttle vector for Magnetospirillum magneticum.
A 3.7-kb cryptic plasmid designated pMGT was found in Magnetospirillum magneticum MGT-1. It was characterized and used for the development of an improved expression system in strain AMB-1 through the construction of a shuttle vector, pUMG. An electroporation method for magnetic bacteria that uses the cryptic plasmid was also developed.
Okamura
Yoshiko
Y
Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16 Koganei, Tokyo.
Takeyama
Haruko
H
Sekine
Takumi
T
Sakaguchi
Toshifumi
T
Wahyudi
Aris Tri
AT
Sato
Rika
R
Kamiya
Shinji
S
Matsunaga
Tadashi
T
D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D018274:Electroporation; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005822:Genetic Vectors; D010957:Plasmids / Q000235:genetics*; D012093:Replicon; D012244:Rhodospirillaceae / Q000235:genetics*