title: Chemiluminescence determination of melatonin and some of its derivatives using potassium permanganate and formaldehyde system.
abstract: It was found that melatonin and its derivatives, such as N-acetyl- 5-methoxytryptamine (MT), N-acetyl-5-hydroxytryptamine (NAS), 5-Methoxytrypt- amine (5-MT), 5-Methoxyindolyl acetic acid (5-MIAA) and N-acetyl-5-methoxy- 6-hydroxytryptamine (6-HMT) would give chemiluminescence in the acidic potassium permanganate solution, and formaldehyde would enhance this chemiluminescent reaction greatly. The optimum conditions for this chemiluminescent reaction were studied in detail by a flow injection system. A new simple rapid method has been developed under the optimum conditions for determination of melatonin. This method has the advantages of high sensitivity, wide range of linear response and low detection limit. On the basis of investigation of chemiluminescent, fluorescent and UV spectra of melatonin in acidic solution containing potassium permanganate and formaldehyde, a possible mechanism of this reaction was proposed.
authors.affiliation: Department of Chemistry, Fuzhou University, 350002, Fuzhou, Fujian, China. gnchen@fzu.edu.cn
authors.forename: Guo Nan
authors.initials: GN
authors.lastname: Chen
authors.forename: Fu Xin
authors.initials: FX
authors.lastname: Huang
authors.forename: Xiao Ping
authors.initials: XP
authors.lastname: Wu
authors.forename: Zheng Feng
authors.initials: ZF
authors.lastname: Zhao
authors.forename: Jian Ping
authors.initials: JP
authors.lastname: Duan
mesh_terms: D017022:Flow Injection Analysis; D005557:Formaldehyde / Q000737:chemistry*; D007700:Kinetics; D008163:Luminescent Measurements; D008550:Melatonin / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry; D015394:Molecular Structure; D011196:Potassium Permanganate / Q000737:chemistry*
chemical_list: D011196:Potassium Permanganate; D005557:Formaldehyde; D008550:Melatonin