Effects of tributyltin chloride on the reproductive system in pubertal male rats.
Detrimental effects of tributyltin (TBT) chloride on the reproductive system were investigated in pubertal male rats. Sixty Sprague-Dawley rats aged with 35 days were assigned to six different groups; negative control receiving vehicle, positive control receiving methyltestosterone (10 mg/kg B.W.), TBT chloride (5 mg/kg B.W., 10 mg/kg B.W., and 20 mg/kg B.W.), and a combination of TBT chloride (10 mg/kg B.W.) and flutamide (10 mg/kg B.W). The animals were treated with test compounds by oral gavage daily for 10 days and sacrificed on the next day of the final treatment. The treatment with TBT chloride at the doses of 10 and 20 mg/kg B.W. significantly decreased seminal vesicle weights, compared to the negative control. The combined treatment of TBT chloride and flutamide caused a significant decrease in accessory sex organ weights, compared to the control and TBT chloride treatments. The treatment with TBT chloride or in the combination with flutamide increased detached debris and sloughed cells in the tubules of epididymis and narrowed seminal vesicles. In addition, the combined treatment with TBT chloride and flutamide caused a noticeable increase in serum androgen level, compared to the negative control. These results suggest that TBT chloride exposed during pubertal period cause partial reproductive disorders in male rats.
Yu
Wook-joon
WJ
College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Korea.
Nam
Sang-yoon
SY
Kim
Young-chul
YC
Lee
Beom-jun
BJ
Yun
Young-won
YW
D000818:Animals; D001835:Body Weight; D004822:Epididymis / Q000187:drug effects; D005485:Flutamide / Q000494:pharmacology; D005837:Genitalia, Male / Q000187:drug effects*; D008297:Male; D008777:Methyltestosterone / Q000494:pharmacology; D009929:Organ Size; D011467:Prostate / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012669:Seminal Vesicles / Q000187:drug effects; D012741:Sexual Maturation / Q000187:drug effects; D013737:Testis / Q000187:drug effects; D014220:Trialkyltin Compounds / Q000494:pharmacology*
D014220:Trialkyltin Compounds; C011559:tributyltin; D005485:Flutamide; D008777:Methyltestosterone