title: [Isolation and primary structure of trypsin from the red king crab Paralithodes camtschaticus].
abstract: Trypsin from hepatopancreas of the Paralithodes camtschaticus crab was isolated in homogeneous state by successive ion-exchange chromatography on DEAE-Sephadex, affinity chromatography on Agarose modified with peptide ligands from trypsin hydrolysate of salmin, and ion-exchange chromatography on a Mono Q column. The total yield of the protein was 64%. Its N-terminal amino acid sequence was determined (IVGGTEVTPG-). A sample of amplified total cDNA of hepatopancreas of king crab was obtained. The cDNA fragment containing the complete coding part of the gene was isolated on the basis of the known N-terminal amino acid sequence of the mature form of the trypsin. The polypeptide chain of the proenzyme consists of 266 aa. The mature trypsin involves 237 aa, which corresponds to its molecular mass of 24.8 kDa. A comparison of the amino acid sequence of the king crab trypsin with those of trypsins from other species of crustaceans demonstrated their high structural homology. The trypsin from the Penaeus vannamei shrimp appeared to be the most close in its primary structure to that of the king crab (70% identity).
authors.affiliation: Chemical Faculty, Moscow State University, Vorob'evy gory, Moscow, 119899 Russia.
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Kislitsyn
authors.forename: D V
authors.initials: DV
authors.lastname: Rebrikov
authors.forename: Ia E
authors.initials: IaE
authors.lastname: Dunaevskiĭ
authors.forename: G N
authors.initials: GN
authors.lastname: Rudenskaia
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D033381:Anomura / Q000201:enzymology* / Q000235:genetics; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004064:Digestive System / Q000201:enzymology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D014357:Trypsin / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*
chemical_list: D014357:Trypsin