Quantitative proteomic analysis of chromatin-associated factors.
A method to identify and quantify chromatin-associated proteins has been developed and applied to the analysis of changes in chromatin-associated proteins induced by Myc oncoprotein expression in human B lymphocytes. Chromatin-enriched fractions were isolated by differential detergent/salt extraction and analyzed by ICAT reagent labeling, multi-dimensional chromatography and tandem mass spectrometry. Many known chromatin-associated regulatory factors were identified and quantified. The method will be widely applicable to various biological systems and reveal changes in chromatin-associated regulatory factors that underlie biological phenomena.
Shiio
Yuzuru
Y
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA.
Eisenman
Robert N
RN
Yi
Eugene C
EC
Donohoe
Sam
S
Goodlett
David R
DR
Aebersold
Ruedi
R
D001402:B-Lymphocytes / Q000737:chemistry / Q000166:cytology; D002460:Cell Line; D002843:Chromatin / Q000737:chemistry*; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis / Q000235:genetics; D016259:Genes, myc / Q000235:genetics; D006801:Humans; D009687:Nuclear Proteins / Q000032:analysis* / Q000235:genetics; D020543:Proteome / Q000032:analysis* / Q000235:genetics; D015854:Up-Regulation
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D009687:Nuclear Proteins; D020543:Proteome