Type XIII collagen and some other transmembrane collagens contain two separate coiled-coil motifs, which may function as independent oligomerization domains.
Type XIII collagen is a homotrimeric transmembrane collagen composed of a short intracellular domain, a single membrane-spanning region, and an extracellular ectodomain with three collagenous domains (COL1-3) separated by short non-collagenous domains (NC1-4). Several collagenous transmembrane proteins have been found to harbor a conserved sequence next to their membrane-spanning regions, and in the case of type XIII collagen this sequence has been demonstrated to be important for chain association. We show here that this 21-residue sequence is necessary but not sufficient for NC1 association. Furthermore, the NC1 association region was predicted to form an alpha-helical coiled-coil structure, which may already begin at the membrane-spanning region, as is also predicted for the related collagen types XXIII and XXV. Interestingly, a second coiled-coil structure is predicted to be located in the NC3 domain of type XIII collagen and in the corresponding domains of types XXIII and XXV. It is found experimentally that the absence of the NC1 coiled-coil domain leads to a lack of disulfide-bonded trimers and misfolding of the membrane-proximal collagenous domain COL1, whereas the COL2 and COL3 domains are correctly folded. We suggest that the NC1 coiled-coil domain is important for association of the N-terminal part of the type XIII collagen alpha chains, whereas the NC3 coiled-coil domain is implicated in the association of the C-terminal part of the molecule. All in all, we propose that two widely separated coiled-coil domains of type XIII and related collagens function as independent oligomerization domains participating in the folding of distinct areas of the molecule.
Latvanlehto
Anne
A
Collagen Research Unit, Biocenter, University of Oulu, P. O. Box 5000, FIN-90014 Oulu, Finland.
Snellman
Anne
A
Tu
Hongmin
H
Pihlajaniemi
Taina
T
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D003094:Collagen / Q000737:chemistry*; D024181:Collagen Type XIII / Q000737:chemistry*; D004220:Disulfides / Q000737:chemistry; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry*; D051379:Mice; D008969:Molecular Sequence Data; D010434:Pepsin A / Q000494:pharmacology; D017510:Protein Folding; D011994:Recombinant Proteins / Q000737:chemistry
D024181:Collagen Type XIII; D004220:Disulfides; D008565:Membrane Proteins; D011994:Recombinant Proteins; D003094:Collagen; D010434:Pepsin A