Ca2+ imaging of mouse neocortical interneurone dendrites: Ia-type K+ channels control action potential backpropagation.
GABAergic interneurones are essential in cortical processing, yet the functional properties of their dendrites are still poorly understood. In this first study, we combined two-photon calcium imaging with whole-cell recording and anatomical reconstructions to examine the calcium dynamics during action potential (AP) backpropagation in three types of V1 supragranular interneurones: parvalbumin-positive fast spikers (FS), calretinin-positive irregular spikers (IS), and adapting cells (AD). Somatically generated APs actively backpropagated into the dendritic tree and evoked instantaneous calcium accumulations. Although voltage-gated calcium channels were expressed throughout the dendritic arbor, calcium signals during backpropagation of both single APs and AP trains were restricted to proximal dendrites. This spatial control of AP backpropagation was mediated by Ia-type potassium currents and could be mitigated by by previous synaptic activity. Further, we observed supralinear summation of calcium signals in synaptically activated dendritic compartments. Together, these findings indicate that in interneurons, dendritic AP propagation is synaptically regulated. We propose that interneurones have a perisomatic and a distal dendritic functional compartment, with different integrative functions.
Goldberg
Jesse H
JH
Department of Biological Sciences, Columbia University, 1212 Amsterdam Avenue, New York, NY 10027, USA. jhg24@columbia.edu
Tamas
Gabor
G
Yuste
Rafael
R
D000200:Action Potentials; D000818:Animals; D002118:Calcium / Q000378:metabolism* / Q000502:physiology; D015220:Calcium Channels / Q000502:physiology; D003712:Dendrites / Q000378:metabolism* / Q000502:physiology; D004553:Electric Conductivity; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology; D007395:Interneurons / Q000145:classification / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D019579:Neocortex / Q000166:cytology / Q000378:metabolism*; D015221:Potassium Channels / Q000502:physiology*; D013569:Synapses / Q000502:physiology; D014793:Visual Cortex / Q000166:cytology / Q000378:metabolism
D015220:Calcium Channels; D015221:Potassium Channels; D002118:Calcium