Generation of a monoclonal antibody against the mouse Sf3b1 (SAP155) gene product for U2 snRNP component of spliceosome.
SAP155 is a subunit of the U2 snRNP, and plays an important role for prespliceosome assembly and splicing catalysis of the major spliceosome. Recently, it was reported that SAP155 was also a subunit of the minor spliceosome. These suggest that SAP155 is essential for the removal of any type of intron. More recently, a homolog of SAP155 cDNA, designated Sf3b1, was isolated from mouse. In this study, we report the generation of a monoclonal antibody (MAb) against murine Sf3b1 protein. This MAb recognizes endogenous Sf3b1 gene product by Western blotting, but less efficiently by immunoprecipitation.
Horie
Atsuya
A
Department of Molecular Embryology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan.
Isono
Kyoichi
K
Koseki
Haruhiko
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology*; D000918:Antibody Specificity; D051379:Mice; D010750:Phosphoproteins / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D000072260:RNA Splicing Factors; D017413:Ribonucleoprotein, U2 Small Nuclear / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D017381:Spliceosomes / Q000737:chemistry
D000911:Antibodies, Monoclonal; D010750:Phosphoproteins; D000072260:RNA Splicing Factors; D017413:Ribonucleoprotein, U2 Small Nuclear; C491799:Sf3b1 protein, mouse