title: The solution structure of YbcJ from Escherichia coli reveals a recently discovered alphaL motif involved in RNA binding.
abstract: The structure of the recombinant Escherichia coli protein YbcJ, a representative of a conserved family of bacterial proteins (COG2501), was determined by nuclear magnetic resonance. The fold of YbcJ identified it as a member of the larger family of S4-like RNA binding domains. These domains bind to structured RNA, such as that found in tRNA, rRNA, and a pseudoknot of mRNA. The structure of YbcJ revealed a highly conserved patch of basic residues, comprising amino acids K26, K38, R55, K56, and K59, which likely participate in RNA binding.
authors.affiliation: Department of Biochemistry, McGill University, Montreal, Quebec, H3G 1Y6, Canada.
authors.forename: Laurent
authors.initials: L
authors.lastname: Volpon
authors.forename: Carine
authors.initials: C
authors.lastname: Lievre
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Osborne
authors.forename: Shaifali
authors.initials: S
authors.lastname: Gandhi
authors.forename: Pietro
authors.initials: P
authors.lastname: Iannuzzi
authors.forename: Robert
authors.initials: R
authors.lastname: Larocque
authors.forename: Miroslaw
authors.initials: M
authors.lastname: Cygler
authors.forename: Kalle
authors.initials: K
authors.lastname: Gehring
authors.forename: Irena
authors.initials: I
authors.lastname: Ekiel
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D011487:Protein Conformation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012329:RNA, Bacterial / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry; D012996:Solutions
chemical_list: D029968:Escherichia coli Proteins; D012329:RNA, Bacterial; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; D012996:Solutions; C476148:YbcJ protein, E coli