Inflammatory infiltrate of chronic periradicular lesions: an immunohistochemical study.
OBJECTIVE
To determine the cellular profile of human chronic periradicular lesions using immunohistochemical methods in order to study the differences in the cell infiltrate of periradicular granulomas and cysts.


METHODS
The study population consisted of 45 individuals without any systemic disease. Biopsies were obtained during periradicular surgery. Paraffin-embedded sections were stained by the avidin-biotin complex method (ABC), whilst cryostat tissue sections were stained using the alkaline phosphatase antialkaline phosphatase assay (APAAP). These methods are highly valid and sensitive using a panel of specific monoclonal antibodies: CD4, CD8, CD3, CD10, HLADR, CD20, CD45RO, CD68 and CD57. The 45 specimens were characterized by the use of both techniques.


RESULTS
The 45 specimens were histologically diagnosed as: 25 periradicular granulomas, 17 periradicular cysts and 3 scar tissues. No statistically significant differences were detected in the inflammatory infiltrate between periradicular granulomas and cysts. Observation of the sections showed that the majority of inflammatory cells consisted of T and B lymphocytes and macrophages. T and B lymphocytes were equally distributed in 60% of the cases. The T4/T8 ratio ranged approximately from 1 to 3 and greater, being consistent with inflammation of periradicular tissues. The final differentiation of B lymphocytes to plasma cells was also detected, whilst natural killer (NK) cells were found in only 10 cases (22%). Moreover, antigen presenting cells and T suppressor/cytotoxic cells were found to be associated with both pre-existing and newly formed epithelium.


CONCLUSIONS
Periradicular granulomas and cysts represent two different stages in the development of chronic periradicular pathosis as a normal result of the process of immune reactions that cannot be inhibited.
Liapatas
S
S
Dental School, Athens University, and Pathology Department, 'Evangelismos' General Hospital, Athens, Greece. sliapatas@yahoo.gr
Nakou
M
M
Rontogianni
D
D
D000293:Adolescent; D000328:Adult; D000469:Alkaline Phosphatase / Q000032:analysis; D000911:Antibodies, Monoclonal; D000938:Antigen-Presenting Cells / Q000473:pathology; D001402:B-Lymphocytes / Q000473:pathology; D016516:CD4-CD8 Ratio; D002908:Chronic Disease; D002921:Cicatrix / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D007694:Killer Cells, Natural / Q000473:pathology; D008264:Macrophages / Q000473:pathology; D010483:Periapical Diseases / Q000473:pathology*; D010484:Periapical Granuloma / Q000473:pathology; D010950:Plasma Cells / Q000473:pathology; D011842:Radicular Cyst / Q000473:pathology; D013601:T-Lymphocytes / Q000473:pathology; D013602:T-Lymphocytes, Cytotoxic / Q000473:pathology; D050378:T-Lymphocytes, Regulatory / Q000473:pathology
D000911:Antibodies, Monoclonal; D000469:Alkaline Phosphatase