Cloning and overexpression of the avi2 gene encoding a major cellulase produced by Humicola insolens FERM BP-5977.
The avi2 gene encoding Avi2, which is a major cellulase produced by Humicola insolens FERM BP-5977, was cloned and sequenced. Avi2 showed high homology with other family 6 cellulases. The expression vector pNCE4 containing the avi2 gene was constructed, and this strain was transformed using a protoplast method. As a result, the pNCE4 transformant secreted 8-fold more Avi2 than the recipient strain.
Moriya
Tatsuki
T
Microbiological Resources and Technology Laboratories, Meiji Seika Kaisya Ltd., 788 Kayama, Odawara-shi 250-0852, Japan. tatsuki_moriya@meiji.co.jp
Watanabe
Manabu
M
Sumida
Naomi
N
Okakura
Kaoru
K
Murakami
Takeshi
T
D044602:Cellulases / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004271:DNA, Fungal; D005822:Genetic Vectors; D003904:Mitosporic Fungi / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014170:Transformation, Genetic
D004271:DNA, Fungal; D044602:Cellulases