Studies on Bacillus stearothermophilus. Part III. Transformation of testosterone.
Bacillus stearothermophilus, a thermophilic bacterium isolated from the Kuwaiti desert, produced a variety of monohydroxy androstene derivatives and an oxidized product when incubated with exogenous testosterone for 24 h at 65 degrees C. The major metabolite was identified as androst-4-en-3,17-dione while minor metabolites included 6 alpha-hydroxyandrost-4-en-3,17-dione, 6 beta-hydroxyandrost-4-en-3,17-dione, 6 alpha-hydroxytestosterone, and 6 beta-hydroxytestosterone. These metabolites were purified by TLC and HPLC followed by their identification using (1)H- and (13)C-NMR and other spectroscopic data.
Al-Awadi
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S
Department of Biological Sciences, Biochemistry Program, Faculty of Science, Kuwait University, P.O. Box 5969, 13060 Safat, Kuwait. sameera@kuc0l.kuniv.edu.kw
Afzal
M
M
Oommen
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S
D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D001411:Geobacillus stearothermophilus / Q000378:metabolism*; D006900:Hydroxylation; D006915:Hydroxytestosterones / Q000032:analysis / Q000302:isolation & purification; D008958:Models, Molecular; D013739:Testosterone / Q000378:metabolism*
D006915:Hydroxytestosterones; D013739:Testosterone