Light activated patterning of dye-labeled molecules on surfaces.
This Communication describes a novel method for patterning proteins, ligands, or other organic/inorganic species onto solid substrates. The process works by linking the moiety of interest to a fluorophore that can be subsequently photobleached and bound to the interface. The technique can be performed in aqueous solution and allows several species to be addressed onto the surface simultaneously by using different frequencies of light. Because the method can be used with fluorophores excited by visible light, shorter and more damaging wavelengths can be avoided.
Holden
Matthew A
MA
Department of Chemistry, Texas A&M University, College Station, TX 77843, USA.
Cremer
Paul S
PS
D002623:Chemistry Techniques, Analytical / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry; D009930:Organic Chemicals; D010777:Photochemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013499:Surface Properties
C417664:Alexa594; D005456:Fluorescent Dyes; D007074:Immunoglobulin G; D009930:Organic Chemicals; D012710:Serum Albumin, Bovine