Genetic transformation of Dichanthium annulatum (Forssk)--an apomictic tropical forage grass.
Eleven Dichanthium annulatum (Forssk) plants were regenerated from embryogenic callus co-transformed with two plasmids encoding either the hygromycin phosphotransferase gene (hph) or the beta-glucuronidase (GUS) gene (uidA). Analysis of these putative transformants showed that three plants were transformed with the hph gene, showed the presence of the hph transcript and expressed hygromycin resistance after transfer to soil. Two of these also contained the uidA gene but did not express GUS and were shown to be the same transformation event. All three of the transformants set seed. Hygromycin resistance varied from 68-100% in the progeny of the three transformants. Transgene transmission appeared to have been mainly through apomixis.
Dalton
S J
SJ
Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, Ceredigion SY23 3EB, UK. sue.dalton@bbsrc.ac.uk
Bettany
A J E
AJ
Bhat
V
V
Gupta
M G
MG
Bailey
K
K
Timms
E
E
Morris
P
P
D015139:Blotting, Southern; D002934:Cinnamates; D046508:Culture Techniques; D015870:Gene Expression; D005966:Glucuronidase / Q000235:genetics; D006921:Hygromycin B / Q000031:analogs & derivatives* / Q000494:pharmacology; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics; D030821:Plants, Genetically Modified; D006109:Poaceae / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D012038:Regeneration; D012098:Reproduction; D012641:Selection, Genetic; D012987:Soil; D014170:Transformation, Genetic; D019076:Transgenes / Q000235:genetics
D002934:Cinnamates; D012987:Soil; D006921:Hygromycin B; C026273:hygromycin A; D017853:Phosphotransferases (Alcohol Group Acceptor); C063242:hygromycin-B kinase; D005966:Glucuronidase