Comparative mapping of twenty-eight bovine loci in sheep (Ovis aries, 2n = 54) and river buffalo (Bubalus bubalis, 2n = 50) by FISH.
Peripheral blood cell cultures were treated for late incorporation of both BrdU and Hoechst-33258 to obtain R-banding pattern preparations. Twenty-eight bovine cosmids from 19 bovine syntenic groups (U), three of which contain type I loci and 25 which contain microsatellite loci and have previously been assigned to cattle chromosomes, were comparatively FISH-mapped to sheep and river buffalo chromosomes according to the standard karyotypes (13 loci for the first time in the latter species). The results enrich the physical maps of both species with information relative to the following loci and to the corresponding syntenic groups: IDVGA35 and IDVGA53 (U6), IDVGA61 and IDVGA84 (U13), JAB10 (U5), IDVGA41 and IDVGA57 (U27), IDVGA87 (U11), IDVGA32 and IDVGA10 (U19), IDVGA49, IDVGA66 and IDVGA68 (U1), ZNF164 (U23), IDVGA74 and IDVGA70 (U9), IDVGA47, IDVGA46 and IDVGA58 (U21), MAP1B (U14), IDVGA79 (U4), CATHL (U12), IDVGA71 (U8), IDVGA59 (U26), IDVGA29 (U29), IDVGA7 (U7), IDVGA82 (X), IDVGA50 (Y). All mapped loci were localized on homoeologous chromosomes and chromosome regions of the two species, confirming the high degree of chromosome homoeologies between the subfamilies Bovinae and Caprinae.
Di Meo
G P
GP
Laboratory of Animal Cytogenetics and Gene Mapping, National Research Council (CNR), ISPAAM, Via Argine 1085, I-80147 Naples, Italy.
Perucatti
A
A
Incarnato
D
D
Ferretti
L
L
Di Berardino
D
D
Caputi Jambrenghi
A
A
Vonghia
G
G
Iannuzzi
L
L
D000818:Animals; D002020:Buffaloes / Q000235:genetics*; D002874:Chromosome Mapping; D003360:Cosmids; D005819:Genetic Markers; D017404:In Situ Hybridization, Fluorescence; D012756:Sheep / Q000235:genetics*
D005819:Genetic Markers