Identification and characterization of KAT, a novel gene preferentially expressed in several human cancer cell lines.
We describe the molecular characterization of a novel human gene on chromosome 1q23.3, termed KAT, which is highly conserved among mammals. The KAT gene spans a genomic region of approximately 1.6 kilobases and consists of 4 exons encoding a 115 amino acid protein with a molecular mass of about 12.5 kDa. The gene is expressed in several human tissues, including kidney, liver, skeletal muscle, heart, colon, thymus, spleen, placenta and lung. We identified an alternatively spliced form, lacking exon 2, in human and mouse tissues. In silico analysis of expressed sequence tags, derived from different types of human tumors, revealed another splice variant. This transcript is characterized by retention of the third intron, leading to a truncated translation product. The KAT protein is localized around the nuclear membranes. It was found to be expressed in several breast, colon and lung carcinoma cell lines, but not in normal breast epithelial cell lines. In addition, KAT protein was detected in invasive ductal carcinoma, but not in adjacent tissues. This suggests a role of this gene in tumorigenesis.
Wenzel
Katrin
K
Max Delbrück Center for Molecular Medicine, HELIOS Kliniken GmbH, Franz-Volhard-Klinik, Charité, Humboldt Universität, Berlin, Germany.
Felix
Stephan B
SB
Flachmeier
Christina
C
Heere
Petra
P
Schulze
Wolfgang
W
Grunewald
Ingrid
I
Pankow
Harald
H
Hewelt
Andreas
A
Scherneck
Siegfried
S
Bauer
David
D
Hoehe
Margret R
MR
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D018270:Carcinoma, Ductal, Breast / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000235:genetics; D045744:Cell Line, Tumor; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics*; D004268:DNA-Binding Proteins; D005787:Gene Frequency; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics*; D009369:Neoplasms / Q000235:genetics* / Q000378:metabolism; D009685:Nuclear Envelope / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics; D024363:Transcription Initiation Site; D051720:Upstream Stimulatory Factors
D015815:Cell Adhesion Molecules; D004268:DNA-Binding Proteins; C407243:F11R protein, human; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; C481306:TSTD1 protein, human; D014157:Transcription Factors; D051720:Upstream Stimulatory Factors