A polymorphic, telomeric-like sequence microsatellite in the Neotropical fish Prochilodus.
A microsatellite locus from the Neotropical fish genus Prochilodus was isolated using PCR-based isolation of microsatellite arrays. Of 470 positive clones, 15 were sequenced, and 10 of them showed an (AATTT)(n) repeat. Primers were designed, and analysis of polymorphism revealed 11 alleles in three Prochilodus species. Fluorescence in situ hybridization analysis showed signals predominantly in the telomeric regions of several chromosomes. The description of this microsatellite may contribute to studies of the population structure of this fish group.
Hatanaka
T
T
Departamento de Genética e Evolução, Universidade Federal de São Carlos, Rodovia Washington Luiz Km 235, CEP 13565-905 São Carlos, SP, Brazil.
Henrique-Silva
F
F
Galetti
P M
PM
D000818:Animals; D005399:Fishes / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D018895:Microsatellite Repeats / Q000235:genetics*; D013045:Species Specificity; D016615:Telomere / Q000235:genetics*; D014329:Tropical Climate