title: Evaluation of a new fiber-optic pressure recording system for cardiovascular measurements in mice.
abstract: We have tested a new fiber-optic pressure recording system, Samba, with a thin fiber [outer diameter (OD) = 0.25 mm] and a pressure sensor (length and OD = 0.42 mm) attached to the end. The accuracy of the system tested in vitro was good, with a coefficient of variation of 2.54% at 100 mmHg. The drift was <0.45 mmHg/h, and the temperature sensitivity was approximately 0.07 mmHg/1 degrees C between 22 and 37 degrees C. The frequency response characteristics were similar to a 1.4-Fr Millar catheter (0-200 Hz). Introduction of the Samba sensor from the right carotid artery into the left ventricle in six mice caused no drop in mean aortic pressure, whereas introduction of a 1.4-Fr Millar catheter (OD = 0.47 mm; n = 6) caused a pressure drop from 91.6 +/- 9.2 to 65.1 +/- 6.2 mmHg; P < 0.05. Thus the Samba sensor system may represent a new alternative to assess hemodynamic variables in the murine cardiovascular system.
authors.affiliation: Institute for Experimental Medical Research, Ullevål University Hospital, University of Oslo, 0407 Oslo, Norway.
authors.forename: Per Reidar
authors.initials: PR
authors.lastname: Woldbaek
authors.forename: Taevje Andreas
authors.initials: TA
authors.lastname: Stromme
authors.forename: Jørn Bodvar
authors.initials: JB
authors.lastname: Sande
authors.forename: Geir
authors.initials: G
authors.lastname: Christensen
authors.forename: Theis
authors.initials: T
authors.lastname: Tønnessen
authors.forename: Arnfinn
authors.initials: A
authors.lastname: Ilebekk
mesh_terms: D000818:Animals; D001011:Aorta / Q000502:physiology*; D001794:Blood Pressure / Q000502:physiology*; D001795:Blood Pressure Determination / Q000295:instrumentation* / Q000592:standards; D005336:Fiber Optic Technology / Q000295:instrumentation* / Q000592:standards; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D055100:Optical Fibers; D015203:Reproducibility of Results; D013696:Temperature