[Effects of hypoxic preconditioning on the adhesion of neutrophils to vascular endothelial cells induced by hypoxia/reoxygenation].
OBJECTIVE
To investigate whether hypoxic preconditioning (HPC) can inhibit neutrophils (PMN) adhesion to vascular endothelial cell (VEC).


METHODS
The adhesion of PMN to VEC was measured by endogenous enzyme hexosaminidase. The effect of HPC on the expression of endothelial cell (EC) adhesion molecules was determined with flow cytometry.


RESULTS
After stimulated with 1 hour of hypoxia (H) followed by 4 hours of reoxygenation (R), VEC showed an enhanced PMN adhensivity in association with an increased surface expression of E-selectin and intracellular adhesion molecules-1 (ICAM-1). HPC suppressed the expression of E-selectin and ICAM-1 with a subsequent inhibition of PMN adhesion to hypoxia/reoxygenation (H/R) stimulated VEC.


CONCLUSIONS
HPC inhibits PMN adhesion to VEC through regulating the expression of EC adhesion molecules.
Zhou
Si-gui
SG
Institute of Pharmacy and Pharmacology, Nanhua University, Hengyang 421001, Hunan, China.
Lei
Xiao-yong
XY
Liao
Duan-fang
DF
D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D015687:Cell Hypoxia / Q000502:physiology*; D002460:Cell Line; D019040:E-Selectin / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D009504:Neutrophils / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D010100:Oxygen / Q000494:pharmacology*; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
D019040:E-Selectin; D018799:Intercellular Adhesion Molecule-1; D001619:beta-N-Acetylhexosaminidases; D010100:Oxygen