High-speed simultaneous ion-exclusion/cation-exchange chromatography of anions and cations on a weakly acidic cation-exchange resin column.
The simultaneous ion-exclusion/cation-exchange separation column packed with a polymethacrylate-based weakly acidic cation-exchange resin of 3 microm particle size was used to achieve the simultaneous high-speed separation of anions and cations (Cl(-), NO3(-), SO4(2-), Na(+), K(+), NH4(+), Ca(2+) and Mg(2+)) commonly found in environmental samples. The high-speed simultaneous separation is based on a combination of the ion-exclusion mechanism for the anions and the cation-exchange mechanism for cations. The complete separation of the anions and cations was achieved in 5 min by elution with 15 mM tartaric acid-2.5 mM 18-crown-6 at a flow-rate of 1.5 ml/min. Detection limits at S/N=3 ranged from 0.36 to 0.68 microM for anions and 0.63-0.99 microM for cations. This method has been applied to the simultaneous determination of anions and cations in several environmental waters with satisfactory results.
Mori
Masanobu
M
National Institute of Advanced Industrial Science and Technology at Seto, 110 Nishiibara-cho, Seto, Aichi 489-0884, Japan. masanobu-mori@aist.go.jp
Tanaka
Kazuhiko
K
Helaleh
Murad I H
MI
Xu
Qun
Q
Ikedo
Mikaru
M
Ogura
Yutaka
Y
Sato
Shinji
S
Hu
Wenzhi
W
Hasebe
Kiyoshi
K
Haddad
Paul R
PR
D000838:Anions / Q000302:isolation & purification*; D002138:Calibration; D002411:Cation Exchange Resins; D002412:Cations / Q000302:isolation & purification*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D043844:Crown Ethers; D004784:Environmental Monitoring; D006863:Hydrogen-Ion Concentration; D011786:Quality Control; D015203:Reproducibility of Results; D013644:Tartrates; D014867:Water / Q000032:analysis
D000838:Anions; D002411:Cation Exchange Resins; D002412:Cations; D043844:Crown Ethers; D013644:Tartrates; D014867:Water; C015762:18-crown-6; C029768:tartaric acid