Immune response after oral administration of the encapsulated malaria synthetic peptide SPf66.
The synthetic peptide SPf66 adsorbed on alum is one of the few Plasmodium falciparum vaccines which have been tested in field trials. We previously reported that subcutaneous administration of SPf66 loaded PLGA microparticles (MP) enhances the antibody response to this antigen compared to the conventional alum formulation. We now evaluate the suitability of polymeric formulations to obtain systemic immune responses by gastric intubation of Balb/c mice. Formulations composed of 1:1 mixtures of PLGA 50:50 and 75:25 (lactic:glycolic) microparticles were administered by the oral route, and when animals were boosted 3 weeks later significant systemic IgG antibody responses were elicited, comparable to alum triple shot and superior to the aqueous vaccine given by the oral route. The finding of IgG2a isotype for PLGA-vaccinated mice compared to the absent levels of this isotype for the alum-vaccinated group could be interpreted as a sign of Th1-like immune response and cellular immune response activation. Our results confirm that using the appropriate schedule the oral administration of PLGA particles is suitable to obtain systemic immune responses to the carried antigen.
Carcaboso
A M
AM
Pharmacy and Pharmaceutical Technology Laboratory, Pharmacy Faculty, University of the Basque Country (UPV-EHU), Paseo de la Universidad no 7, 01006 Vitoria-Gasteiz, Spain.
Hernández
R M
RM
Igartua
M
M
Gascón
A R
AR
Rosas
J E
JE
Patarroyo
M E
ME
Pedraz
J L
JL
D000276:Adjuvants, Immunologic; D000284:Administration, Oral; D000534:Alum Compounds; D000818:Animals; D004334:Drug Administration Schedule; D004337:Drug Carriers; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D007279:Injections, Subcutaneous; D019344:Lactic Acid / Q000737:chemistry; D017780:Malaria Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008855:Microscopy, Electron, Scanning; D010316:Particle Size; D010963:Plasmodium falciparum / Q000276:immunology; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry; D015800:Protozoan Proteins / Q000008:administration & dosage* / Q000276:immunology*; D011994:Recombinant Proteins / Q000008:administration & dosage* / Q000276:immunology*; D013499:Surface Properties
D000276:Adjuvants, Immunologic; D000534:Alum Compounds; D004337:Drug Carriers; D007074:Immunoglobulin G; D017780:Malaria Vaccines; D011108:Polymers; D015800:Protozoan Proteins; D011994:Recombinant Proteins; C066269:SPf66 protein, Plasmodium; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C041524:aluminum sulfate