title: Natural N-terminal fragments of brain abundant myristoylated protein BASP1.
abstract: BASP1 (also known as CAP-23 and NAP-22) is a novel myristoylated calmodulin-binding protein, abundant in nerve terminals. It is considered as a signal protein participating in neurite outgrowth and synaptic plasticity. BASP1 is also present in significant amounts in kidney, testis, and lymphoid tissues. In this study, we show that BASP1 is accompanied by at least six BASP1 immunologically related proteins (BIRPs), which are present in all animal species studied (rat, bovine, human, chicken). BIRPs have lower molecular masses than that of BASP1. Similarly to BASP1, they are myristoylated. Peptide mapping and partial sequencing have shown that BIRPs represent a set of BASP1 N-terminal fragments devoid of C-terminal parts of different length. In a definite species, the same set of BASP1 fragments is present in both brain and other tissues. The sum amount of the fragments is about 50% of the BASP1 amount in a tissue. Obligatory accompanying of BASP1 by a set of specific fragments indicates that these fragments are of physiological significance.
authors.affiliation: Molecular and Radiation Biophysics Division, Petersburg Nuclear Physics Institute of Russian Academy of Sciences, 188300 Gatchina, Leningrad District, Russia.zakharov@vz5518.spb.edu
authors.forename: Vladislav V
authors.initials: VV
authors.lastname: Zakharov
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Capony
authors.forename: Jean
authors.initials: J
authors.lastname: Derancourt
authors.forename: Ekaterina S
authors.initials: ES
authors.lastname: Kropolova
authors.forename: Vera A
authors.initials: VA
authors.lastname: Novitskaya
authors.forename: Marina N
authors.initials: MN
authors.lastname: Bogdanova
authors.forename: Mark I
authors.initials: MI
authors.lastname: Mosevitsky
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D006801:Humans; D015151:Immunoblotting; D008565:Membrane Proteins; D019814:Myristic Acid / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D010446:Peptide Fragments / Q000032:analysis* / Q000737:chemistry; D010449:Peptide Mapping; D051381:Rats; D012097:Repressor Proteins
chemical_list: C104089:BASP1 protein, human; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; D012097:Repressor Proteins; D019814:Myristic Acid