Production of tobacco aroma from lutein. Specific role of the microorganisms involved in the process.
A mixed culture formed by Bacillus sp. and Geotrichum sp. produced tobacco aroma compounds from the carotenoid lutein through the formation of the intermediate beta-ionone. Both microorganisms can grow independently in a medium supplemented with lutein, but only Geotrichum produces beta-ionone. This intermediate was incorporated by the bacilli, converted to aroma and this product excreted to the culture medium. Bacillus sp. did not utilize beta-ionone for growth but modified it. We conclude that, in the bioconversion of lutein to products with tobacco aroma, Geotrichum sp. is involved in carotenoid oxidation to produce beta-ionone and Bacillus sp. is responsible for the norisoprenoid reduction to produce 7,8-dihydro-beta-ionone and 7,8-dihydro-beta-ionol.
Maldonado-Robledo
G
G
Departamento de Biología Molecular y Biotecnología del Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México., México, DF 04510, México.
Rodriguez-Bustamante
E
E
Sanchez-Contreras
A
A
Rodriguez-Sanoja
R
R
Sanchez
S
S
D001407:Bacillus / Q000254:growth & development / Q000378:metabolism*; D005848:Geotrichum / Q000254:growth & development / Q000378:metabolism*; D014975:Lutein / Q000378:metabolism*; D045792:Norisoprenoids / Q000378:metabolism*; D009812:Odorants; D011506:Proteins / Q000032:analysis; D014026:Nicotiana
D045792:Norisoprenoids; D011506:Proteins; C008157:beta-ionone; D014975:Lutein