CFD-aided design of a dynamic filter for mammalian cell separation.
In the present work, a rotating disk filter was designed for mammalian cell separation with the aim of avoiding both cell damage and membrane fouling. Different geometric and operational variables of the rotating disk filter were studied using computational fluid dynamics (CFD) by varying rotor radius, rotor angle, membrane-rotor distance, and angular velocity. The combinations of these variables followed a statistical design, so that an analysis of the CFD results provided correlations describing the average shear stress on the membrane surface and the maximum shear stress in the whole module as a function of the variables studied. Based on these correlations, and on the shear resistance levels of Chinese hamster ovary (CHO) and baby hamster kidney (BHK) cell lines, which were investigated using a cone-and-plate viscosimeter, it was possible to determine the geometry and angular velocity that would minimize both cell damage and membrane fouling. After construction, the filter was tested in filtration experiments at increasing permeate fluxes. Cell viability remained >90% for the duration of the experiments (2.5 h), and no indication of fouling was observed. It was shown that the designed dynamic filter is able to effectively avoid both cell damage and membrane fouling, and thus can be used for mammalian cell harvesting and perfusion.
Castilho
Leda R
LR
Biochemical Engineering Division, GBF-German Research Centre for Biotechnology, Braunschweig, Germany. leda@ufrj.br
Anspach
F Birger
FB
D000818:Animals; D000831:Animals, Newborn; D016466:CHO Cells / Q000166:cytology; D002452:Cell Count; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods*; D002460:Cell Line; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods*; D002470:Cell Survival / Q000502:physiology; D003198:Computer Simulation; D006224:Cricetinae; D003412:Cricetulus; D004867:Equipment Design / Q000379:methods; D019544:Equipment Failure Analysis; D008322:Mammals; D008567:Membranes, Artificial; D044085:Microfluidics / Q000379:methods*; D008954:Models, Biological; D014462:Ultrafiltration / Q000295:instrumentation* / Q000379:methods*
D008567:Membranes, Artificial