betaFSH, betaLH and growth hormone gene expression in blue gourami (Trichogaster trichopterus, Pallas 1770) during spermatogenesis and male sexual behavior.
The relationship between gonadal development (histological evidence for spermiogenesis and/or spermatogenesis), sexual behavior (nest-building) and mRNA levels of gonadotropins (betaFSH and betaLH) and growth hormone (GH) in the male pituitary was investigated. Amplification of betaFSH cDNA showed a significantly higher mRNA level in mature males (whether sexually active or not) than in juveniles. However, following PCR amplification of betaLH cDNA, a significantly higher mRNA level was found in the sexually active group compared to the sexually inactive group. These results suggest that FSH may participate in spermatogenesis, whereas LH is more involved in spermiogenesis. The GH mRNA level increased slightly during the maturation process but no significant differences were found between the groups studied.
Degani
Gad
G
School of Science and Technology, Tel-Hai Academic College, Israel. gad@migal.co.il
Jackson
Karen
K
Goldberg
Doron
D
Sarfati
Raheli
R
Avtalion
Ramy R
RR
D000818:Animals; D017931:DNA Primers; D037201:Follicle Stimulating Hormone, beta Subunit / Q000096:biosynthesis* / Q000494:pharmacology; D005786:Gene Expression Regulation; D013006:Growth Hormone / Q000096:biosynthesis* / Q000494:pharmacology; D037101:Luteinizing Hormone, beta Subunit / Q000096:biosynthesis* / Q000494:pharmacology; D008297:Male; D010473:Perciformes / Q000502:physiology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012333:RNA, Messenger / Q000032:analysis; D012726:Sexual Behavior, Animal; D013091:Spermatogenesis / Q000502:physiology*; D013737:Testis / Q000254:growth & development
D017931:DNA Primers; D037201:Follicle Stimulating Hormone, beta Subunit; D037101:Luteinizing Hormone, beta Subunit; D012333:RNA, Messenger; D013006:Growth Hormone