Mutations in actin-related proteins 2 and 3 affect cell shape development in Arabidopsis.
ACTIN-RELATED PROTEINS 2 and 3 form the major subunits of the ARP2/3 complex, which is known as an important regulator of actin organization in diverse organisms. Here, we report that two genes, WURM and DISTORTED1, which are important for cell shape control in Arabidopsis, encode the plant ARP2 and ARP3 orthologs, respectively. Mutations in these genes result in misdirected expansion of various cell types: trichome expansion is randomized, pavement cells fail to produce lobes, hypocotyl cells curl out of the normal epidermal plane, and root hairs are sinuous. At the subcellular level, cell shape changes are linked to severe filamentous actin aggregation and compromised vacuole fusion. Because all seven subunits of the ARP2/3 complex are present in plants, our data indicate that this complex may play a pivotal role during plant cell morphogenesis.
Mathur
Jaideep
J
Botanical Institute III, University of Köln, D 50931 Köln, Germany. jaideep.mathur@uni-koeln.de
Mathur
Neeta
N
Kernebeck
Birgit
B
Hülskamp
Martin
M
D051377:Actin-Related Protein 2; D051376:Actin-Related Protein 2-3 Complex; D051378:Actin-Related Protein 3; D000199:Actins / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D048429:Cell Size / Q000235:genetics / Q000502:physiology; D022081:Cell Surface Extensions / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism; D018546:Hypocotyl / Q000235:genetics / Q000254:growth & development; D008840:Microfilament Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D009154:Mutation; D019441:Plant Epidermis / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development; D017386:Sequence Homology, Amino Acid; D013844:Thiazoles / Q000494:pharmacology; D053778:Thiazolidines; D014617:Vacuoles / Q000187:drug effects / Q000378:metabolism
C512742:ARP2 protein, Arabidopsis; D051377:Actin-Related Protein 2; D051376:Actin-Related Protein 2-3 Complex; D051378:Actin-Related Protein 3; D000199:Actins; D029681:Arabidopsis Proteins; D019086:Bridged Bicyclo Compounds, Heterocyclic; D003598:Cytoskeletal Proteins; C478027:DIS1 protein, Arabidopsis; D008840:Microfilament Proteins; D013844:Thiazoles; D053778:Thiazolidines; C478980:WURM protein, Arabidopsis; C037068:latrunculin B