In situ identification of intracellular bacteria related to Paenibacillus spp. in the mycelium of the ectomycorrhizal fungus Laccaria bicolor S238N.
Bacterial proliferations have recurrently been observed for the past 15 years in fermentor cultures of the ectomycorrhizal fungus Laccaria bicolor S238N, suggesting the presence of cryptic bacteria in the collection culture of this fungus. In this study, intracellular bacteria were detected by fluorescence in situ hybridization in combination with confocal laser scanning microscopy in several collection subcultures of L. bicolor S238N. They were small (0.5 micro m in diameter), rare, and heterogeneously distributed in the mycelium and were identified as Paenibacillus spp. by using a 16S rRNA-directed oligonucleotide probe initially designed for bacteria isolated from a fermentor culture of L. bicolor S238N.
Bertaux
J
J
Institut National de la Recherche Agronomique, UMR "Interactions Arbres/Micro-organismes," Centre de Nancy, 54280 Champenoux, France.
Schmid
M
M
Prevost-Boure
N Chemidlin
NC
Churin
J L
JL
Hartmann
A
A
Garbaye
J
J
Frey-Klett
P
P
D000363:Agaricales / Q000166:cytology / Q000254:growth & development*; D001406:Bacillaceae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D003470:Culture Media; D015342:DNA Probes; D005285:Fermentation; D017404:In Situ Hybridization, Fluorescence; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D025282:Mycelium / Q000254:growth & development*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D003470:Culture Media; D015342:DNA Probes; D012336:RNA, Ribosomal, 16S