Assessment of utility of meiosis-associated promoters of lily for induction of germinal ds transposition in transgenic rice.
In order to suppress the somatic excision of the Ds element and increase the independent transposition events of the Ac/Ds transposon tagging system in rice, we employed promoters of two meiosis-specific genes of lily, LIM10 and LIM18. The LIM10 promoter directed GUS expression specifically in anthers, with the LIM18 promoter doing the same in the anthers and somatic tissue. Both promoters induced independent germinal transposition with the frequency of approximately 1%. The LIM10 promoter, lacking induction of somatic transposition, is considered to be useful for improving transposon-tagging efficiencies in rice.
Morita
Ryouhei
R
Laboratory of Plant Breeding and Genetics, Graduate School of Agricultural Science, Tohoku University, Sendai, 981-8555 Japan.
Hattori
Yoko
Y
Yokoi
Shuji
S
Takase
Hisabumi
H
Minami
Masayoshi
M
Hiratsuka
Kazuyuki
K
Toriyama
Kinya
K
D004251:DNA Transposable Elements / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D018525:Germination / Q000235:genetics*; D027762:Lilium / Q000235:genetics*; D008540:Meiosis / Q000235:genetics*; D012275:Oryza / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000254:growth & development / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*
D004251:DNA Transposable Elements