title: Structure and dynamics of adenosine loops in RNA bulge duplexes as revealed by linked application of thermodynamics, spectrofluorimetry and simulation of molecular dynamics.
abstract: Structure and dynamics of adenosine loops in RNA bulge duplexes was studied using time-resolved spectrofluorimetry and in aqua simulation of molecular dynamics. Thermodynamics revealed that 2-aminopurine riboside is an non-invasive fluorescent probe when built within the bulge region of chemically synthesized RNA duplexes.
authors.affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, 61-704 Poznań, Noskowskiego 12/14, Poland.
authors.forename: T
authors.initials: T
authors.lastname: Kuliński
authors.forename: L
authors.initials: L
authors.lastname: Bielecki
authors.forename: R W
authors.initials: RW
authors.lastname: Adamiak
mesh_terms: D015075:2-Aminopurine / Q000737:chemistry; D000241:Adenosine / Q000737:chemistry*; D001483:Base Sequence; D003198:Computer Simulation; D005456:Fluorescent Dyes / Q000737:chemistry; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry*; D012330:RNA, Double-Stranded / Q000138:chemical synthesis / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics; D013997:Time Factors
chemical_list: D005456:Fluorescent Dyes; D012330:RNA, Double-Stranded; D015075:2-Aminopurine; D012313:RNA; D000241:Adenosine