title: Expression of Bcl-2 on leukocytes in prostatic fluid from patients with acute and chronic prostatitis.
abstract: In our continuing investigation to clarify the significance of leukocytosis and its prolongation in prostatic fluid (PF) from prostatitis patients, we investigated whether expression of the antiapoptotic gene Bcl-2 on leukocytes in prostatic fluid has any relation with prolonged leukocytosis in prostatitis. The subjects were eight patients with acute bacterial prostatitis (ABP) and eight with nonbacterial prostatitis (NBP) with a history of more than 2 years. Twelve micrograms of protein obtained from leukocytes in PF were subjected to Western blot analysis. Bcl-2 was expressed in seven of eight (87.5%) and six of eight (75.0%) patients with NBP and ABP, respectively. Quantitative density analysis of the Bcl-2 band did not show any statistically significant differences between patients with NBP and those with ABP. These results suggest that the expression of antiapoptotic Bcl-2 on leukocytes in PF is not a cause of leukocyte longevity in long-term prostatitis.
authors.affiliation: Department of Urology, Nippon Medical School, Tokyo, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Abe
authors.forename: Taiji
authors.initials: T
authors.lastname: Nishimura
authors.forename: Sadamitsu
authors.initials: S
authors.lastname: Asoh
authors.forename: Shigeo
authors.initials: S
authors.lastname: Ohta
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D017209:Apoptosis; D002908:Chronic Disease; D006801:Humans; D007962:Leukocytes / Q000737:chemistry / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000502:physiology; D011467:Prostate / Q000737:chemistry*; D011472:Prostatitis / Q000097:blood*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000097:blood*
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2