[Influence of transfected EGFR-cDNA on bcl-2 and Bax in glioblastoma cells].
OBJECTIVE
To investigate the correlation of bcl-2 and Bax protein with nuclear matrix in glioblastoma cell line U87 as well as the effect of EGFR-cDNA transfection on the expression of bcl-2 and Bax in U87 cells.


METHODS
The correlation of bcl-2 and Bax protein with nuclear matrix in glioblastoma cell line U87 was studied by confocal microscopy and Western blot. The expression of bcl-2 and Bax in EGFR-cDNA transfected and untransfected glioblastoma cell lines was studied by Western blot.


RESULTS
Confocal microscopic images showed that bcl-2 protein was localized in the periphery of the nuclear matrix and Bax in the nuclear matrix. A 26 kDa bcl-2 band and a specific band of Bax at about 66 000 were detected in nuclear matrix proteins by western blot. The expression of bcl-2 was lower but that of Bax was higher in EGFR-cDNA transfected cells than the control.


CONCLUSIONS
Bcl-2 and Bax, being nuclear matrix associated proteins, are probably involved in the EGFR-cDNA induced malignant conversion of glioblastoma cells by introducing EGFR cDNA into the tumor cells.
Wang
Zi-hui
ZH
Department of Pathology, Shenzhen Hospital, Beijing University, Shenzhen 518036, China.
Yu
Ding
D
Hao
Jian-zhong
JZ
D017209:Apoptosis; D045744:Cell Line, Tumor; D066246:ErbB Receptors / Q000235:genetics* / Q000502:physiology; D005909:Glioblastoma / Q000473:pathology*; D006801:Humans; D015530:Nuclear Matrix / Q000737:chemistry; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis* / Q000502:physiology; D014162:Transfection; D051028:bcl-2-Associated X Protein / Q000032:analysis* / Q000502:physiology
C494826:BAX protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D066246:ErbB Receptors