title: Modulo is a target of Myc selectively required for growth of proliferative cells in Drosophila.
abstract: In Drosophila, the homologue of the proto-oncogene Myc is a key regulator of both cell size and cell growth. The identities and roles of dMyc target genes in these processes, however, remain largely unexplored. Here, we investigate the function of the modulo (mod) gene, which encodes a nucleolus localized protein. In gain of function or loss of function experiments, we demonstrate that mod is directly controlled by dMyc. Strikingly, in proliferative imaginal cells, mod loss-of-function impairs both cell growth and cell size, whereas larval endoreplicative tissues grow normally. In contrast to dMyc, over-expressing Mod in wing imaginal discs is not sufficient to induce cell growth. Taken together, our results indicate that mod does not possess the full spectrum of dMyc activities, but is required selectively in proliferative cells to sustain their growth and to maintain their specific size.
authors.affiliation: Institut de Génétique Humaine, 141 rue de la Cardonille, 34396 Montpellier Cedex 5, France. perrin@ibdm.univ-mrs.fr
authors.forename: Laurent
authors.initials: L
authors.lastname: Perrin
authors.forename: Corinne
authors.initials: C
authors.lastname: Benassayag
authors.forename: Dominique
authors.initials: D
authors.lastname: Morello
authors.forename: Jacques
authors.initials: J
authors.lastname: Pradel
authors.forename: Jacques
authors.initials: J
authors.lastname: Montagne
mesh_terms: D000818:Animals; D001483:Base Sequence; D002455:Cell Division / Q000502:physiology*; D002466:Cell Nucleolus / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004330:Drosophila / Q000254:growth & development / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D024721:E-Box Elements; D005786:Gene Expression Regulation; D007814:Larva / Q000254:growth & development / Q000378:metabolism; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D016271:Proto-Oncogene Proteins c-myc; D016601:RNA-Binding Proteins; C117962:mod protein, Drosophila