Oxygen tension distribution in postcapillary venules in resting skeletal muscle.
We tested the hypothesis that blood flow is distributed among capillary networks in resting skeletal muscle in such a manner as to maintain uniform end-capillary PO2. Oxygen tension in venules draining two to five capillaries was obtained by using the phosphorescence decay methodology in rat spinotrapezius muscle. For 64 postcapillary venules among 18 networks in 10 animals, the mean PO2 was 30.1 Torr (range, 9.7-43.5 Torr) with a coefficient of variation (CV; standard deviation/mean) of 0.26. Oxygen levels of postcapillary venules within a single network or single animal, however, displayed a much smaller CV (0.064 and 0.094, respectively). By comparison, the CV of blood flow in 57 postcapillary venules of 17 networks in 9 animals was 1.27 with a mean flow of 0.011 +/- 0.014 nl/s and a range of 3.7 x 10(-4) to 6.5 x 10(-2) nl/s. Blood flow of postcapillary venules within single networks displayed a lower CV (mean, 0.51), whereas that in individual animals was 0.78. Results indicate that among venular networks, heterogeneity of oxygen tension is less than that of blood flow and within venular networks the heterogeneity of oxygen tension is much less than that of blood flow. In addition, postcapillary PO2 was independent of flow among venules in which both were measured. Results of this study may be attributable to three factors: 1) O2 diffusion between adjacent capillaries and venules, 2) structural remodeling in regions of lower PO2, and 3) O2-dependent local control mechanisms.
Saltzman
Darin J
DJ
Department of Bioengineering, University of California-San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0412, USA.
Toth
Andras
A
Tsai
Amy G
AG
Intaglietta
Marcos
M
Johnson
Paul C
PC
D000818:Animals; D001810:Blood Volume / Q000502:physiology; D008297:Male; D018482:Muscle, Skeletal / Q000098:blood supply* / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D010313:Partial Pressure; D051381:Rats; D017208:Rats, Wistar; D012039:Regional Blood Flow / Q000502:physiology; D012146:Rest / Q000502:physiology*; D014699:Venules / Q000378:metabolism
D010100:Oxygen