title: Detection of Filifactor alocis in endodontic infections associated with different forms of periradicular diseases.
abstract: Recent molecular studies have expanded the list of suspected endodontic pathogens. The aim of this study was to evaluate the occurrence of Filifactor alocis in primary endodontic infections associated with different forms of periradicular diseases. Identification by nested polymerase chain reaction was performed in root canal samples from teeth associated with either asymptomatic periradicular lesions or acute apical periodontitis. Samples were also taken by aspiration of purulent exudate associated with acute apical abscesses. DNA extracted from the samples was initially amplified using universal 16S rDNA primers followed by a second round of amplification using the first PCR products to detect a specific fragment of F. alocis 16S rDNA. F. alocis was detected in 12/21 (57.1%) root canal samples from teeth showing asymptomatic periradicular lesions and in 3/10 (30%) samples taken from root canals associated with acute apical periodontitis. This species occurred in 8/19 (42.1%) pus aspirates obtained from abscessed teeth. In general, F. alocis was detected in 23/50 (46%) samples taken from endodontic infections. These findings suggest that F. alocis is involved in the etiology of different forms of periradicular diseases and has the potential to be an endodontic pathogen.
authors.affiliation: Department of Endodontics, School of Dentistry, Estácio de Sá University, Brazil. siqueira@estacio.br
authors.forename: J F
authors.initials: JF
authors.lastname: Siqueira
authors.forename: I N
authors.initials: IN
authors.lastname: Rôças
mesh_terms: D000293:Adolescent; D000328:Adult; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D003790:Dental Pulp Necrosis / Q000382:microbiology*; D005673:Fusobacterium / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D006801:Humans; D010483:Periapical Diseases / Q000382:microbiology*; D016133:Polymerase Chain Reaction
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal