title: [Effects of hypoxic preconditioning on the adhesion of neutrophils to vascular endothelial cells induced by hypoxia/reoxygenation].
abstract: OBJECTIVE
  To investigate whether hypoxic preconditioning (HPC) can inhibit neutrophils (PMN) adhesion to vascular endothelial cell (VEC).
  
  
  METHODS
  The adhesion of PMN to VEC was measured by endogenous enzyme hexosaminidase. The effect of HPC on the expression of endothelial cell (EC) adhesion molecules was determined with flow cytometry.
  
  
  RESULTS
  After stimulated with 1 hour of hypoxia (H) followed by 4 hours of reoxygenation (R), VEC showed an enhanced PMN adhensivity in association with an increased surface expression of E-selectin and intracellular adhesion molecules-1 (ICAM-1). HPC suppressed the expression of E-selectin and ICAM-1 with a subsequent inhibition of PMN adhesion to hypoxia/reoxygenation (H/R) stimulated VEC.
  
  
  CONCLUSIONS
  HPC inhibits PMN adhesion to VEC through regulating the expression of EC adhesion molecules.
authors.affiliation: Institute of Pharmacy and Pharmacology, Nanhua University, Hengyang 421001, Hunan, China.
authors.forename: Si-gui
authors.initials: SG
authors.lastname: Zhou
authors.forename: Xiao-yong
authors.initials: XY
authors.lastname: Lei
authors.forename: Duan-fang
authors.initials: DF
authors.lastname: Liao
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D015687:Cell Hypoxia / Q000502:physiology*; D002460:Cell Line; D019040:E-Selectin / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D009504:Neutrophils / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D010100:Oxygen / Q000494:pharmacology*; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
chemical_list: D019040:E-Selectin; D018799:Intercellular Adhesion Molecule-1; D001619:beta-N-Acetylhexosaminidases; D010100:Oxygen