Induction mechanism of 3-hydroxy-3-methylglutaryl-CoA reductase in potato tuber and sweet potato root tissues.
3-Hydroxy-3-methylglutaryl coenzyme A reductase (HMGR, EC1.1.1.34), the key enzyme in isoprenoid biosynthesis, was purified from microsomes of potato tuber tissue, and a polyclonal antibody and two monoclonal antibodies against the purified enzyme were prepared. HMGR protein content was measured by immunotitration and radioimmunoassay using these antibodies. HMGR activity was very low in the fresh tissues of both potato tuber and sweet potato root. The activity in potato tuber was increased by cutting and further by additional fungal infection of the cut tissues. In sweet potato root tissue, the activity was scarcely increased after cutting alone, but was markedly increased by additional fungal infection or chemical treatment. The HMGR protein contents in both fresh potato tuber and sweet potato root tissues were also very low, and increased markedly in response to cutting and fungal infection. From these results, we proposed a hypothesis on the induction mechanism of HMGR after cutting and fungal infection in potato tuber and sweet potato root tissues.
Kondo
Katsuyoshi
K
Laboratory of Food Biochemistry, Nagoya Women's University, 3-40 Shioji, Mizuho, Nagoya 467-8610, Japan.
Uritani
Ikuzo
I
Oba
Kazuko
K
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001345:Autoradiography; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction; D006825:Hybridomas; D006903:Hydroxymethylglutaryl CoA Reductases / Q000096:biosynthesis*; D007120:Immunochemistry; D027723:Ipomoea batatas / Q000201:enzymology*; D008297:Male; D008627:Mercuric Chloride / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D008861:Microsomes / Q000201:enzymology; D008970:Molecular Weight; D009181:Mycoses / Q000201:enzymology / Q000382:microbiology; D010935:Plant Diseases; D018517:Plant Roots / Q000201:enzymology; D011863:Radioimmunoassay; D011198:Solanum tuberosum / Q000201:enzymology*
D000911:Antibodies, Monoclonal; D008627:Mercuric Chloride; D006903:Hydroxymethylglutaryl CoA Reductases