Rice ubiquitin promoters: deletion analysis and potential usefulness in plant transformation systems.
Strong constitutive promoters form a cornerstone for basic and applied research using transgenic plants. GUS (beta-glucuronidase) expression levels from constructs containing RUBQ1 or RUB2 rice ubiquitin promoters were 8- to 35-fold higher in transgenic rice [Oryza sativa (L.)] plants, respectively, when compared to the 35S promoter. Deletion analysis of the 5'-upstream region of RUBQ2 revealed a putative enhancer region that produced a 2.4-fold increase in transient GUS expression. Southern blot analysis showed that three to seven copies of the GUS gene were stably inserted into R0 and R1 plants and inherited in a monogenic fashion.
Wang
J
J
Louisiana Agricultural Experiment Station, LSU Agricultural Center, Louisiana State University, Baton Rouge, LA 70803, USA.
Oard
J H
JH
D015139:Blotting, Southern; D046508:Culture Techniques; D018744:DNA, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D012275:Oryza / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014170:Transformation, Genetic; D014452:Ubiquitins / Q000235:genetics*
D018744:DNA, Plant; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D014452:Ubiquitins; D005966:Glucuronidase