Effects of benzo[a]pyrene, 2-bromopropane, phenol and 2,3,7,8-tetrachlorodibenzo-p-dioxin on proinflammatory cytokines gene expression by mice spleen cells.
The detrimental effects of environmental pollutants on the health of the individual are generally accepted, although the mechanisms of these effects remain to be incompletely understood. In the present study, we examined the effects of B[a]P, 2-BP, phenol and TCDD on proinflammatory cytokine gene expression in mice spleen cells which were stimulated with anti-CD3. 10(-9)M TCDD increased IFNgamma and TNFalpha gene expression, but suppressed IL-1 gene expression. 10(-6)M phenol inhibited IL-1, IL-6 and TNFalpha gene expression, and 10(-6)M of 2-BP downregulated TNFalpha gene expression. However, 10(-6)M of B[a]P did not influence on IL-1, IL-6, IFNgamma and TNFalpha gene expression. These findings suggest that TCDD may impair the immune functions of mice by enhancing proinflammatory cytokines production, whereas phenol and 2-BP may impair the functions by inhibiting the production of these cytokines.
Ho-Jun
Kim
K
College of Veterinary Medicine, Chungnam National University, Daejeon 305-764, Korea.
Kang
Bit-Na
BN
Cho
Sung-Whan
SW
Son
Hwa-Young
HY
Jeong
Kyu-Shik
KS
Park
Sang-Joon
SJ
Kim
Sung-Ho
SH
Kim
Se-Ra
SR
Kim
Tae-Hwan
TH
An
Mi-Young
MY
Ryu
Si-Yun
SY
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001564:Benzo(a)pyrene / Q000633:toxicity*; D017252:CD3 Complex / Q000276:immunology; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D004785:Environmental Pollutants / Q000633:toxicity*; D015870:Gene Expression / Q000187:drug effects; D006842:Hydrocarbons, Brominated / Q000633:toxicity*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D019800:Phenol / Q000633:toxicity*; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity*; D012313:RNA / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000187:drug effects* / Q000378:metabolism
D017252:CD3 Complex; D016207:Cytokines; D004785:Environmental Pollutants; D006842:Hydrocarbons, Brominated; D000072317:Polychlorinated Dibenzodioxins; D019800:Phenol; D001564:Benzo(a)pyrene; D012313:RNA; C103736:2-bromopropane