title: Oligofructose does not affect the development of type 1 diabetes mellitus induced by dietary proteins in the diabetes-prone BB rat model.
abstract: BACKGROUND
  Prevention of Type 1 diabetes mellitus (T1DM), a major childhood chronic disease with rapidly increasing incidence, is an urgent topic of research. We investigated whether 5% oligofructose (OF) as compared to 5% cellulose had a protective effect against diet-induced T1DM in the diabetes-prone BioBreeding (BB) rat model.
  
  
  METHODS
  Groups of BB rats were fed the experimental diets from weaning. The diets were a cereal-based rodent diet (diabetogenic, positive control) and semi-synthetic rodent diets containing hydrolysed casein (non-diabetogenic, negative control), soy or whey as the sole protein source and 5% cellulose as fibre source. In additional groups fed soy and whey protein, the fibre source was 5% OF. T1DM incidence up to the age of 160 days was recorded applying biochemical and morphological criteria. Physiological effects of fibre were assessed through the analysis of biochemical parameters in plasma and of the protein/DNA ratio in intestinal mucosa.
  
  
  RESULTS
  T1DM incidence was diet-dependent. Cereal-, soy- and whey-based diets were significantly more diabetogenic than the hydrolysed casein-based diet. Five per cent OF did not affect the incidence of T1DM induced by either soy or whey proteins as compared to cellulose, nor induce any of the biological effects attributed to a fermentable fibre.
  
  
  CONCLUSIONS
  In the BB rat model, 5% OF in the diet did not have any protective effects against diet-induced T1DM. The present data do not suggest dietary OF as a promising approach for the dietary prevention of T1DM.
authors.affiliation: Department of Quality and Safety Assurance, Nestec Ltd. Research Center, Lausanne, Switzerland. irene.perrin@rdls.nestle.com
authors.forename: I V
authors.initials: IV
authors.lastname: Perrin
authors.forename: M
authors.initials: M
authors.lastname: Marchesini
authors.forename: F C
authors.initials: FC
authors.lastname: Rochat
authors.forename: E J
authors.initials: EJ
authors.lastname: Schiffrin
authors.forename: B
authors.initials: B
authors.lastname: Schilter
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002364:Caseins / Q000008:administration & dosage; D002482:Cellulose / Q000008:administration & dosage; D003922:Diabetes Mellitus, Type 1 / Q000209:etiology / Q000276:immunology / Q000517:prevention & control*; D004032:Diet / Q000009:adverse effects; D004044:Dietary Proteins / Q000008:administration & dosage*; D004195:Disease Models, Animal; D005260:Female; D015994:Incidence; D007413:Intestinal Mucosa / Q000473:pathology; D008297:Male; D009844:Oligosaccharides / Q000008:administration & dosage*; D051381:Rats; D011913:Rats, Inbred BB
chemical_list: D001786:Blood Glucose; D002364:Caseins; D004044:Dietary Proteins; D009844:Oligosaccharides; C120489:oligofructose; D002482:Cellulose