title: [Targeted therapy of human laryngeal squamous cell carcinoma in vitro with telomerase catalytic subunit mRNA].
abstract: OBJECTIVE
  To study the effect of antisense oligonucleotide targeted human telomerase catalytic subunit (hTERT) on Hep-2 cell line.
  
  
  METHODS
  Cells were treated daily with oligonucleotide (ODN) directed against hTERT, or control ODN-a mismatched sequence or only Lipofectamine. At 24 h, 48 h, 72 h cell viability after administration of ODNs was determined using the MTT assay. At 72 h, the morpholog alteration of the cells was observed by HE stain. At 72 h, telomerase activity was determined.
  
  
  RESULTS
  After daily transfection ASODN, some Hep-2 cells begin to stop growing up, but not in the mismatched ODN group or Lipofectamine group. Hep-2 cells treated with antisense against hTERT showed a decrease in telomerase activity.
  
  
  CONCLUSIONS
  hTERT antisense ODN can inhibit telomerase activity and the growth of Hep-2 cells in short term.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, People's Hospital of Wuhan University, Wuhan 430060.
authors.forename: Shiming
authors.initials: S
authors.lastname: Chen
authors.forename: Zezhang
authors.initials: Z
authors.lastname: Tao
authors.forename: Bokui
authors.initials: B
authors.lastname: Xiao
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Hou
authors.forename: Zhanqiu
authors.initials: Z
authors.lastname: Yang
mesh_terms: D004268:DNA-Binding Proteins; D006801:Humans; D007822:Laryngeal Neoplasms / Q000201:enzymology*; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D019098:Telomerase / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D004268:DNA-Binding Proteins; D020319:Oligodeoxyribonucleotides, Antisense; D019098:Telomerase