title: The release profiles of intact and enzymatically digested hyaluronic acid from semisolid formulations using multi-layer membrane system.
abstract: A multi-layer membrane system was used to measure in vitro release of hydrophilic macromolecules such as hyaluronic acid (HA) from semisolid formulations. One enzymatically digested HA-derivative with molecular mass of 22 kDa (HA-D) and 1200 kDa intact HA (HA) were incorporated into three semisolid formulations: water-containing hydrophilic ointment (WHO), amphiphilic cream (AC) and water-containing wool wax alcohol ointment (WWO). Because of the high hydrophilic properties of HA-D and HA, the artificial model membranes consisted of collodion as the matrix and glycerol as the hydrophilic acceptor phase. The area under the concentration-time curve and the mean dissolution time were used as a quantitative parameter to characterise the rate and extent of release in vitro. This study showed that the HA-D and HA release as hydrophilic substances from WHO was higher than both from AC and WWO. It was observed that 83% of HA-D1 was released from WHO after 2 h; in contrast, only 10% was released from 2% HA from the same vehicle during the same time. In conclusion, the in vitro availability of enzymatically digested HA-D was higher for WHO than for the other formulations, AC and WWO. Similarly, the availability of HA-D was higher than that of HA from the same formulations.
authors.affiliation: Institute of Pharmaceutics and Biopharmaceutics, Martin-Luther-University Halle-Wittenberg, Halle, Germany.
authors.forename: Jamal Alyoussef
authors.initials: JA
authors.lastname: Alkrad
authors.forename: Yahya
authors.initials: Y
authors.lastname: Mrestani
authors.forename: Reinhard H H
authors.initials: RH
authors.lastname: Neubert
mesh_terms: D002021:Buffers; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid; D003101:Collodion / Q000737:chemistry; D004339:Drug Compounding; D019075:Electrophoresis, Capillary; D004798:Enzymes / Q000737:chemistry*; D005079:Excipients / Q000737:chemistry; D005990:Glycerol / Q000737:chemistry; D006820:Hyaluronic Acid / Q000737:chemistry*; D007700:Kinetics; D008567:Membranes, Artificial; D008970:Molecular Weight; D009824:Ointments; D012995:Solubility; D013997:Time Factors; D014014:Tissue Adhesives / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D002021:Buffers; D004798:Enzymes; D005079:Excipients; D008567:Membranes, Artificial; D009824:Ointments; D014014:Tissue Adhesives; D014867:Water; D006820:Hyaluronic Acid; D003101:Collodion; D005990:Glycerol