title: Detection of Legionella pneumophila by real-time PCR for the mip gene.
abstract: A real-time PCR assay for the mip gene of Legionella pneumophila was tested with 27 isolates of L. pneumophila, 20 isolates of 14 other Legionella species, and 103 non-Legionella bacteria. Eight culture-positive and 40 culture-negative clinical specimens were tested. This assay was 100% sensitive and 100% specific for L. pneumophila.
authors.affiliation: Cleveland Clinic Foundation, Cleveland, Ohio, USA.
authors.forename: Deborah A
authors.initials: DA
authors.lastname: Wilson
authors.forename: Belinda
authors.initials: B
authors.lastname: Yen-Lieberman
authors.forename: Udo
authors.initials: U
authors.lastname: Reischl
authors.forename: Steve M
authors.initials: SM
authors.lastname: Gordon
authors.forename: Gary W
authors.initials: GW
authors.lastname: Procop
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D017931:DNA Primers; D006090:Gram-Negative Bacteria / Q000235:genetics / Q000302:isolation & purification; D006094:Gram-Positive Bacteria / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D020104:Immunophilins / Q000235:genetics*; D007875:Legionella / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D016952:Legionella pneumophila / Q000235:genetics / Q000302:isolation & purification*; D007877:Legionnaires' Disease / Q000382:microbiology; D008565:Membrane Proteins / Q000235:genetics*; D019696:Peptidylprolyl Isomerase; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D008565:Membrane Proteins; D020104:Immunophilins; C512922:Mip protein, Legionella pneumophila; D019696:Peptidylprolyl Isomerase