Synthesis of terminally labelled RNA sequences: fluorescence and hybridisation study of RNA-DNA duplexes.
A stable and suitably labelled DNA-RNA hybrid, resistant to RNAse degradation has been prepared by hybridising the RNA sequence carrying methoxy group at 2'-position of ribose moiety in adenosines and labelled with dansyl group attached via a linker at the terminal uridyl unit. Study may be useful in sequencing and targeting specific viral sequences.
Misra
A
A
Nucleic Acids Research Laboratory, Department of Chemistry, University of Allahabad, Allahabad-211 002, India.
Tripathi
S
S
Misra
K
K
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D003619:Dansyl Compounds / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D046911:Macromolecular Substances; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization / Q000379:methods*; D009843:Oligoribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D012313:RNA / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013696:Temperature
D003619:Dansyl Compounds; D005456:Fluorescent Dyes; D046911:Macromolecular Substances; D009843:Oligoribonucleotides; D012313:RNA; D004247:DNA