title: Inhibition of HIV-1 replication by the combined action of anti-gp41 single chain antibody and IL-16.
abstract: HIV-1 replication is inhibited in T cells transfected with an anti-gp41 single chain antibody (ScFv) or IL-16. These two molecules target totally different events in the HIV-1 replication cycle. The present study shows that HIV-1 replication is inhibited to a substantially greater extent and for a longer duration in cells transfected with both anti-gp41 and IL-16 than with either molecule alone. It is concluded that anti-gp41 and IL-16 act in a synergistic fashion to inhibit HIV-1 replication.
authors.affiliation: Experimental Medicine Section, Oral Immunity and Infection Branch, Building 30, Room 121, NIDCR, National Institutes of Health, 30 Convent Drive, MSC 4322, Bethesda, MD 20892-4322, USA. devadas@cber.fda.gov
authors.forename: Krishnakumar
authors.initials: K
authors.lastname: Devadas
authors.forename: Paul
authors.initials: P
authors.lastname: Zhou
authors.forename: Deepanker
authors.initials: D
authors.lastname: Tewari
authors.forename: Abner Louis
authors.initials: AL
authors.lastname: Notkins
mesh_terms: D000914:Antibodies, Viral / Q000494:pharmacology*; D015704:CD4 Antigens / Q000096:biosynthesis; D002455:Cell Division / Q000187:drug effects; D004357:Drug Synergism; D018274:Electroporation; D015700:HIV Envelope Protein gp41 / Q000276:immunology*; D015497:HIV-1 / Q000187:drug effects*; D006801:Humans; D019410:Interleukin-16 / Q000494:pharmacology*; D019169:Jurkat Cells; D007700:Kinetics; D013050:Spectrometry, Fluorescence; D013601:T-Lymphocytes / Q000821:virology; D014162:Transfection; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000914:Antibodies, Viral; D015704:CD4 Antigens; D015700:HIV Envelope Protein gp41; D019410:Interleukin-16