p130Cas Couples the tyrosine kinase Bmx/Etk with regulation of the actin cytoskeleton and cell migration.
Bmx/Etk, a member of the Tec/Btk family of nonreceptor kinases, has recently been shown to mediate cell motility in signaling pathways that become activated upon integrin-mediated cell adhesion (Chen, R., Kim, O., Li, M., Xiong, X., Guan, J. L., Kung, H. J., Chen, H., Shimizu, Y., and Qiu, Y. (2001) Nat Cell Biol. 3, 439-444). The molecular mechanisms of Bmx-induced cell motility have so far remained unknown. Previous studies by us and others have demonstrated that a complex formation between the docking protein p130Cas (Cas) and the adapter protein Crk is instrumental in connecting several stimuli to the regulation of actin cytoskeleton and cell motility. We demonstrate here that expression of Bmx leads to an interaction between Bmx and Cas at membrane ruffles, which are sites of active actin remodeling in motile cells. Expression of Bmx also enhances tyrosine phosphorylation of Cas and Cas.Crk complex formation, and coexpression of Bmx with Cas results in an enhanced membrane ruffling and haptotactic cell migration. Importantly, a mutant form of Bmx that fails to interact with Cas also fails to induce cell migration. Furthermore, expression of a dominant-negative form of Cas that is incapable of interacting with Crk inhibits Bmx-induced membrane ruffling and cell migration. These studies suggest that Bmx-Cas interaction, phosphorylation of Cas by Bmx, and subsequent Cas.Crk complex formation functionally couple Bmx to the regulation of actin cytoskeleton and cell motility.
Abassi
Yama A
YA
Cancer Research Center, The Burnham Institute, La Jolla, California 92037, USA.
Rehn
Marko
M
Ekman
Niklas
N
Alitalo
Kari
K
Vuori
Kristiina
K
D000199:Actins / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D019556:COS Cells; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology*; D002522:Chlorocebus aethiops; D050719:Crk-Associated Substrate Protein; D016895:Culture Media, Serum-Free; D003599:Cytoskeleton / Q000502:physiology; D004730:Endothelium, Vascular / Q000502:physiology; D006801:Humans; D016297:Mutagenesis, Site-Directed; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D019000:Phosphotyrosine / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D011506:Proteins; D011994:Recombinant Proteins / Q000378:metabolism; D050717:Retinoblastoma-Like Protein p130; D014162:Transfection
D000199:Actins; C493841:BCAR1 protein, human; D050719:Crk-Associated Substrate Protein; D016895:Culture Media, Serum-Free; D010750:Phosphoproteins; D011506:Proteins; D011994:Recombinant Proteins; D050717:Retinoblastoma-Like Protein p130; D019000:Phosphotyrosine; C492367:BMX protein, human; D011505:Protein-Tyrosine Kinases