Resveratrol increases BRCA1 and BRCA2 mRNA expression in breast tumour cell lines.
The phytochemical resveratrol, found in grapes, berries and peanuts, has been found to possess cancer chemopreventive effects by inhibiting diverse cellular events associated with tumour initiation, promotion and progression. Resveratrol is also a phyto-oestrogen, binds to and activates oestrogen receptors that regulate the transcription of oestrogen-responsive target genes such as the breast cancer susceptibility genes BRCA1 and BRCA2. We investigated the effects of resveratrol on BRCA1 and BRCA2 expression in human breast cancer cell lines (MCF7, HBL 100 and MDA-MB 231) using quantitative real-time RT-PCR, and by perfusion chromatography of the proteins. All cell lines were treated with 30 microM resveratrol. The expressions of BRCA1 and BRCA2 mRNAs were increased although no change in the expression of the proteins were found. These data indicate that resveratrol at 30 micro M can increase expression of genes involved in the aggressiveness of human breast tumour cell lines.
Fustier
P
P
Laboratoire d'Oncologie Moléculaire, Centre Jean Perrin, 58 Rue Montalembert, BP 392, 63011 Clermont-Ferrand, France.
Le Corre
L
L
Chalabi
N
N
Vissac-Sabatier
C
C
Communal
Y
Y
Bignon
Y-J
YJ
Bernard-Gallon
D J
DJ
D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D019313:BRCA1 Protein / Q000096:biosynthesis*; D024682:BRCA2 Protein / Q000096:biosynthesis*; D001943:Breast Neoplasms / Q000473:pathology*; D017931:DNA Primers; D004305:Dose-Response Relationship, Drug; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D012333:RNA, Messenger / Q000096:biosynthesis; D000077185:Resveratrol; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013267:Stilbenes / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D016588:Anticarcinogenic Agents; D019313:BRCA1 Protein; D024682:BRCA2 Protein; D017931:DNA Primers; D012333:RNA, Messenger; D013267:Stilbenes; D000077185:Resveratrol