title: Lipase-mediated acidolysis of butteroil with free conjugated linoleic acid in a packed bed reactor.
abstract: Acidolysis of butteroil with free conjugated linoleic acid (CLA) was studied in a packed bed reactor containing an immobilized Candida antarctica (fraction B) lipase. Kinetic data were used to develop quantitative reversible rate expressions of the general Michaelis-Menten form that also incorporate a term for first-order deactivation of the enzyme. The extent of incorporation of CLA in the triacylglycerols of butteroil was characterized for reactions carried out at several temperatures (namely 45 degrees, 50 degrees, and 55 degrees C) with different weight ratios of butteroil to CLA (namely 10:1 and 2:1). At the optimum operating temperature of 50 degrees C, similar levels of incorporation of CLA (60% to 85%) were achieved at low space times (<3 h) for both 10:1 and 2:1 (w/w) ratios of butteroil to CLA.
authors.affiliation: Department of Chemical Engineering, University of Wisconsin-Madison, 1415 Engineering Drive, Madison, Wisconsin 53706, USA.
authors.forename: Prima S
authors.initials: PS
authors.lastname: Sehanputri
authors.forename: Charles G
authors.initials: CG
authors.lastname: Hill
mesh_terms: D019149:Bioreactors / Q000382:microbiology*; D002079:Butter / Q000382:microbiology*; D002175:Candida / Q000254:growth & development* / Q000378:metabolism*; D003198:Computer Simulation; D004042:Dietary Fats, Unsaturated / Q000378:metabolism*; D005240:Feasibility Studies; D005656:Fungal Proteins; D019787:Linoleic Acid / Q000096:biosynthesis*; D008049:Lipase / Q000378:metabolism*; D008954:Models, Biological
chemical_list: D004042:Dietary Fats, Unsaturated; D005656:Fungal Proteins; D002079:Butter; D019787:Linoleic Acid; D008049:Lipase; C431349:lipase B, Candida antarctica