title: Authentic and recombinant bilirubin oxidases are in different resting forms.
abstract: Myrothecium verrucaria bilirubin oxidase expressed in Aspergillus oryzae is in a resting form different from that of the authentic bilirubin oxidase, but reaches the resting form of the authentic enzyme after one cycle of reduction and reoxidation with dioxygen as shown by the absorption and electron paramagnetic resonance spectra.
authors.affiliation: Department of Chemistry, Faculty of Science, Kanazawa University, Kakuma, Kanazawa 920-1192, Japan. ts0513@kenroku.kanazawa-u.ac.jp
authors.forename: Takeshi
authors.initials: T
authors.lastname: Sakurai
authors.forename: Lei
authors.initials: L
authors.lastname: Zhan
authors.forename: Takahiro
authors.initials: T
authors.lastname: Fujita
authors.forename: Kunishige
authors.initials: K
authors.lastname: Kataoka
authors.forename: Atsushi
authors.initials: A
authors.lastname: Shimizu
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Samejima
authors.forename: Shotaro
authors.initials: S
authors.lastname: Yamaguchi
mesh_terms: D001203:Ascomycota / Q000201:enzymology; D001236:Aspergillus oryzae / Q000201:enzymology; D004578:Electron Spin Resonance Spectroscopy; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000737:chemistry* / Q000235:genetics*; D010100:Oxygen / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
chemical_list: D011994:Recombinant Proteins; D044925:Oxidoreductases Acting on CH-CH Group Donors; C042401:bilirubin oxidase; D010100:Oxygen