Sphingosine 1-phosphate signal transduction in muscle cells.
Sphingosine 1-phosphate (S1P) is a powerful bioactive sphingolipid recently recognized to act as extracellular ligand for various subtypes of G protein-coupled receptors belonging to the S1P family. In our study, focused on mouse skeletal muscle cells, we showed that S1P activated enzymes crucial for membrane signal transduction, such as phospholipase D (PLD) and protein kinase C; it promoted also a significant increase of cytosolic Ca2+ via ligation to S1P2 and S1P3 receptor subtypes. Interestingly, myogenic differentiation was found to be accompanied by a profound variation of S1P receptor expression levels and the progressive uncoupling of S1P from PLD activation, suggesting that this signaling pathway is exclusively required for S1P action on proliferating cells.
Meacci
Elisabetta
E
Dipartimento di Scienze Biochimiche, Università di Firenze, Firenze, Italy.
Donati
Chiara
C
Farnararo
Marta
M
Bruni
Paola
P
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism; D008246:Lysophospholipids; D051379:Mice; D008954:Models, Biological; D018482:Muscle, Skeletal / Q000166:cytology; D009132:Muscles / Q000166:cytology*; D011493:Protein Kinase C / Q000378:metabolism; D015398:Signal Transduction; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000378:metabolism*; D015854:Up-Regulation
D008246:Lysophospholipids; C060506:sphingosine 1-phosphate; D011493:Protein Kinase C; D013110:Sphingosine; D002118:Calcium