title: Comparison between two warm ischemic models in experimental liver transplantation in pigs.
abstract: BACKGROUND
  Experimental models of warm ischemia in liver transplantation have been employed to study the mechanisms and treatment of ischemia reperfusion injury.
  
  
  METHODS
  We compared a control group without (group A, n = 10) versus two models of warm ischemia of liver transplants in pigs: namely, occlusion of the hepatic artery and portal vein for 30 minutes (group B, n = 23) and extraction of the liver 60 minutes after cardiac arrest (group C, n = 5). Liver function tests, coagulation studies, and liver biopsies were performed during the first 24 hours post-liver transplant.
  
  
  RESULTS
  Clamping of the hepatic vasculature in group B produced a significant liver injury compared with the control group: elevation of the ALT and an abnormal 1-hour post-revascularization biopsy similar to that observed in the cardiac arrest group C. The transaminase levels were lower among group A animals (P <.05). But the hepatic synthetic functions as reflected in the protrombin time (PT) were not affected in group B versus group A. The alteration in PT with respect to the initial value was similar among group A and group B animals, which were significantly less than that in group C (P <.05).
  
  
  CONCLUSIONS
  Occlusion of the hepatic artery and portal vein, a simple surgical maneuver, causes moderate damage to a liver graft but less alteration of hepatic synthetic function. Clamping of the hepatic vasculture obtains more long-term survivors after OLT than cardiac arrest.
authors.affiliation: General Surgery Department, University Clinic of Navarra, Pamplona, Spain. mregueira@unav.es
authors.forename: F M
authors.initials: FM
authors.lastname: Regueira
authors.forename: A
authors.initials: A
authors.lastname: Espí
authors.forename: P
authors.initials: P
authors.lastname: Nwose
authors.forename: A
authors.initials: A
authors.lastname: Díez-Caballero
authors.forename: J
authors.initials: J
authors.lastname: Baixaulí
authors.forename: F
authors.initials: F
authors.lastname: Rotellar
authors.forename: J
authors.initials: J
authors.lastname: Olea
authors.forename: F
authors.initials: F
authors.lastname: Pardo
authors.forename: J L
authors.initials: JL
authors.lastname: Hernández-Lizoain
authors.forename: J A
authors.initials: JA
authors.lastname: Cienfuegos
mesh_terms: D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D007511:Ischemia; D008099:Liver / Q000166:cytology / Q000473:pathology; D008102:Liver Circulation; D016031:Liver Transplantation / Q000502:physiology*; D023421:Models, Animal; D009926:Organ Preservation / Q000379:methods; D011516:Prothrombin / Q000378:metabolism; D011517:Prothrombin Time; D013552:Swine; D013997:Time Factors; D014184:Transplantation, Homologous
chemical_list: D011516:Prothrombin; D001219:Aspartate Aminotransferases