Biochemical and molecular characterization of a rice glutelin allele for the GluA-1 gene.
The rice ( Oryza sativa L.) mutant of glu4a, lacking the glutelin alpha-2 subunit while the alpha-1 subunit increased (alpha-1H/alpha-2L), was used in this study. Two-dimensional electrophoresis analysis revealed that the mutant lacked the polypeptide pI6.71/alpha-2 encoded by glu4 while forming a new polypeptide of pI6.50/alpha-1. Experiments were conducted to identify the relationships between the mutated polypeptides of the mutant and to illustrate the mutation mechanism of the allele. Peptide mapping and amino-acid sequence analyses revealed that the newly formed glu4a encoded polypeptide pI6.50/alpha-1 of high homology with the deleted pI6.71/alpha-2 polypeptide which was encoded by glu4 (GluA-1). The nucleotide sequence revealed that the iso-electric point variation of the pI6.50/alpha-1 polypeptide was caused by a point mutation with nucleotide replacement at the variable region of the gene. These results suggested the possibility of altering glutelin quality by using single gene mutation.
Qu
L Q
LQ
Institute of Genetics and Developmental Biology, the Chinese Academy of Sciences, Beijing 100101, China. lqu@affrc.go.jp
Wei
X L
XL
Satoh
H
H
Kumamaru
T
T
Ogawa
M
M
Takaiwa
F
F
D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D018744:DNA, Plant / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D005983:Glutens / Q000235:genetics* / Q000378:metabolism; D007526:Isoelectric Point; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D010449:Peptide Mapping; D017354:Point Mutation; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D018744:DNA, Plant; D005983:Glutens