A validated spectrofluorometric method for the determination of celecoxib in capsules.
A rapid, selective, sensitive and simple fluorescence method was developed for the direct determination of celecoxib in capsules. The capsules were emptied, pulverized and dissolved in either ethanol or acetonitrile, sonicated and filtered. Direct fluorescence emission was measured at 355+/-5 nm (exciting at 272 nm). The method was fully validated and the recoveries were excellent, even in presence of excipients.
Damiani
Patricia
P
Departamento de Química Analítica, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, 2000 Rosario, Argentina. pdamiani@infovia.com.ar
Bearzotti
Mariela
M
Cabezón
Miguel A
MA
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis*; D002138:Calibration; D002214:Capsules; D000068579:Celecoxib; D007202:Indicators and Reagents; D015394:Molecular Structure; D011720:Pyrazoles; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods; D013449:Sulfonamides / Q000032:analysis* / Q000737:chemistry
D000894:Anti-Inflammatory Agents, Non-Steroidal; D002214:Capsules; D007202:Indicators and Reagents; D011720:Pyrazoles; D013449:Sulfonamides; D000068579:Celecoxib