title: Design and application of a new cryptic-plasmid-based shuttle vector for Magnetospirillum magneticum.
abstract: A 3.7-kb cryptic plasmid designated pMGT was found in Magnetospirillum magneticum MGT-1. It was characterized and used for the development of an improved expression system in strain AMB-1 through the construction of a shuttle vector, pUMG. An electroporation method for magnetic bacteria that uses the cryptic plasmid was also developed.
authors.affiliation: Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16 Koganei, Tokyo.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Okamura
authors.forename: Haruko
authors.initials: H
authors.lastname: Takeyama
authors.forename: Takumi
authors.initials: T
authors.lastname: Sekine
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Sakaguchi
authors.forename: Aris Tri
authors.initials: AT
authors.lastname: Wahyudi
authors.forename: Rika
authors.initials: R
authors.lastname: Sato
authors.forename: Shinji
authors.initials: S
authors.lastname: Kamiya
authors.forename: Tadashi
authors.initials: T
authors.lastname: Matsunaga
mesh_terms: D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D018274:Electroporation; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005822:Genetic Vectors; D010957:Plasmids / Q000235:genetics*; D012093:Replicon; D012244:Rhodospirillaceae / Q000235:genetics*