[The effect of methylene blue/photochemical method for virus inactivation on plasma components].
Virus inactivation of plasma can be achieved by methylene blue/photochemical method. To investigate the effect of this method on immunological properties and biochemical functions of plasma components, the virus-inactivation method was performed on single-donor plasma that was exposed to visible light (40,000 lux) at room temperature for 1 h in the presence of 1 micro mol/L methylene blue. The results showed that activities of the factor VIII, PT and APTT were decreased to a certain degree while most of other plasma proteins were not affected significantly. Human plasma components including albumin, glucose and minerals as well as plasma pH were also not affected. By using different electrophoreses and immunochemical techniques, no neoantigens were found in photodynamically treated plasma and electrophoretic mobility revealed identical patterns for untreated and treated plasma. In conclusion, methylene blue/photochemical method dose not considerably influence the properties of major of plasma components.
Zhou
Xi-Peng
XP
Institute of Blood Transfusion, Academy of Military Medical Sciences, Beijing 100850, China.
Xu
Jin-Bo
JB
Sun
Ping
P
Yan
Fang
F
Wang
Quan-Li
QL
Bu
Feng-Rong
FR
D001779:Blood Coagulation Factors / Q000378:metabolism; D003176:Complement C3 / Q000378:metabolism; D004586:Electrophoresis / Q000379:methods; D005169:Factor VIII / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008027:Light; D008751:Methylene Blue / Q000494:pharmacology*; D010314:Partial Thromboplastin Time; D010949:Plasma / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D013918:Thrombin Time; D013997:Time Factors; D038301:Virus Inactivation / Q000187:drug effects* / Q000528:radiation effects
D001779:Blood Coagulation Factors; D003176:Complement C3; D005169:Factor VIII; D008751:Methylene Blue