title: Stability and emulsion-forming ability of water-soluble fish myofibrillar protein prepared by conjugation with alginate oligosaccharide.
abstract: Carp myofibrillar protein (Mf) was conjugated with alginate oligosaccharide (AO) through the Maillard reaction under low relative humidity, and the functional properties of the Mf-AO conjugate were investigated under different NaCl concentrations and pH levels. Mf became highly solubilized at lower NaCl concentrations by conjugation with AO, with a slight loss of available lysine. The thermal stability of Mf was effectively improved by conjugation with AO. Heat treatment at 80 degrees C for 2 h had no effect on the solubility of the Mf-AO conjugate attached to 227 microg/mg of AO regardless of the NaCl concentration and pH. Furthermore, the Mf-AO conjugate showed excellent emulsion-forming ability regardless of NaCl concentration. The improved functionalities of Mf by conjugation with AO remained even at a nearly isoelectric point. These results indicate that conjugation with AO through the Maillard reaction is an effective way to prepare high-functional food material from fish muscle protein.
authors.affiliation: Graduate School of Fisheries Sciences, Hokkaido University, Minato 3, Hakodate, Hokkaido 041-8611, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Sato
authors.forename: Shigeru
authors.initials: S
authors.lastname: Katayama
authors.forename: Tomoo
authors.initials: T
authors.lastname: Sawabe
authors.forename: Hiroki
authors.initials: H
authors.lastname: Saeki
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D002347:Carps; D004355:Drug Stability; D004655:Emulsions; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D015416:Maillard Reaction; D009124:Muscle Proteins / Q000737:chemistry*; D009210:Myofibrils / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry*; D012995:Solubility; D014867:Water
chemical_list: D000464:Alginates; D004655:Emulsions; D009124:Muscle Proteins; D009844:Oligosaccharides; D014867:Water