title: N-Myc downstream-regulated gene 2 (NDRG2) inhibits glioblastoma cell proliferation.
abstract: The most severe form of brain glioma, glioblastoma (GBM), is highly malignant and usually resistant to chemotherapy. Therefore, discovery of new targets for gene therapy is important. Using subtraction cloning, we identified the human N-Myc downstream-regulated gene 2 (hNDRG2), located at chromosome 14q11.2, as a gene that is significantly suppressed in GBM tissues. Semiquantitative RT-PCR showed that the hNDRG2 gene transcript is expressed in normal brain tissue and low-grade gliomas but is present at low levels in 15 of 27 (56%) human GBM tissues and all of the 6 human glioblastoma cell lines examined. Furthermore, transfection of human glioblastoma U373 and U138 cells with a cDNA encoding hNDRG2 markedly reduced the cell proliferation. Our findings provide the first evidence to suggest that hNDRG2 may play a role in glioblastoma carcinogenesis.
authors.affiliation: Department of Biochemistry and Molecular Biology, The Fourth Military Medical University, Xi'an, China.
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Deng
authors.forename: Libo
authors.initials: L
authors.lastname: Yao
authors.forename: Ling
authors.initials: L
authors.lastname: Chau
authors.forename: Samuel Sai-ming
authors.initials: SS
authors.lastname: Ng
authors.forename: Ying
authors.initials: Y
authors.lastname: Peng
authors.forename: Xinping
authors.initials: X
authors.lastname: Liu
authors.forename: Wo-shing
authors.initials: WS
authors.lastname: Au
authors.forename: Jicun
authors.initials: J
authors.lastname: Wang
authors.forename: Fuyang
authors.initials: F
authors.lastname: Li
authors.forename: Shaoping
authors.initials: S
authors.lastname: Ji
authors.forename: Hua
authors.initials: H
authors.lastname: Han
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Nie
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.forename: Hsiang-fu
authors.initials: HF
authors.lastname: Kung
authors.forename: Suet-yi
authors.initials: SY
authors.lastname: Leung
authors.forename: Marie Chia-mi
authors.initials: MC
authors.lastname: Lin
mesh_terms: D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D015152:Blotting, Northern; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002455:Cell Division; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics / Q000378:metabolism; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014018:Tissue Distribution; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000473:pathology; D025521:Tumor Suppressor Proteins
chemical_list: D014408:Biomarkers, Tumor; D018076:DNA, Complementary; C466228:NDRG2 protein, human; D009363:Neoplasm Proteins; D011506:Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D025521:Tumor Suppressor Proteins