title: cGMP-phosphodiesterase activity is up-regulated in response to pressure overload of rat ventricles.
abstract: Although expression of natriuretic peptides in cardiac tissues is up-regulated in response to pressure overload, no significant change in cGMP level in hypertrophied ventricles was observed. Activities of two cyclic nucleotide phosphodiesterase (PDE) isoforms, Ca2+/calmodulin-stimulated PDE (PDE1) and cGMP-stimulated PDE (PDE2), were significantly higher in rat left ventricles 14 days after aortic banding. The absence of significant changes in PDE1A and PDE2A mRNA levels indicated that the two PDE activities were post-transcriptionally up-regulated. These results suggested that the increased cGMP-PDE activity in response to pressure overload plays an important role in neutralizing cGMP action in cardiac tissue.
authors.affiliation: Discovery Research Laboratory, Tanabe Seiyaku Co., Ltd., Yodogawa-ku, Osaka 532-8505, Japan.
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Yanaka
authors.forename: Yukie
authors.initials: Y
authors.lastname: Kurosawa
authors.forename: Kouichi
authors.initials: K
authors.lastname: Minami
authors.forename: Eri
authors.initials: E
authors.lastname: Kawai
authors.forename: Kenji
authors.initials: K
authors.lastname: Omori
mesh_terms: D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001011:Aorta / Q000502:physiology; D001794:Blood Pressure / Q000502:physiology*; D015152:Blotting, Northern; D006332:Cardiomegaly / Q000503:physiopathology; D002851:Chromatography, High Pressure Liquid; D015342:DNA Probes; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D006321:Heart / Q000503:physiopathology*; D006352:Heart Ventricles / Q000503:physiopathology; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000201:enzymology*; D009929:Organ Size / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation / Q000502:physiology*; D016276:Ventricular Function
chemical_list: D015342:DNA Probes; D018076:DNA, Complementary; D007527:Isoenzymes; D012333:RNA, Messenger; D015106:3',5'-Cyclic-GMP Phosphodiesterases