Binding activity of replication protein A to single-stranded DNA containing oxidized pyrimidine base.
To obtain the information for the role of replication protein A (RPA) on the detection of oxidized lesion in the single-stranded DNA, the binding preference of RPA purified from Xenopus egg lysate against the oligonucleotide containing one of three kinds of oxidized thymine residues, 5-formyluracil, 5-hydroxymethyluracil and 5-(1,2-dihydroxyethyl)uracil, was studied by the gel shift assay. Results of competition assay indicate that RPA preferentially binds to the oligonucleotide containing these oxidized thymine residues than the undamaged DNA.
Kasama
T
T
Faculty of Science, Kumamoto University, Kumamoto 860-8555, Japan.
Ono
A
A
Izuta
S
S
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D004249:DNA Damage; D004277:DNA, Single-Stranded / Q000737:chemistry* / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism*; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction; D010432:Pentoxyl / Q000031:analogs & derivatives* / Q000737:chemistry; D011743:Pyrimidines / Q000737:chemistry*; D051716:Replication Protein A; D013941:Thymine / Q000031:analogs & derivatives; D014498:Uracil / Q000031:analogs & derivatives* / Q000737:chemistry; D014982:Xenopus laevis
D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D009841:Oligonucleotides; D011743:Pyrimidines; D051716:Replication Protein A; C007777:5-formyluracil; C008055:5-hydroxymethyluracil; D014498:Uracil; D010432:Pentoxyl; D013941:Thymine