title: Proteomic analysis of human vessels: application to atherosclerotic plaques.
abstract: Atherosclerosis is a chronic disease that affects medium and large arteries. This process originates from the interaction between cells of the arterial wall, lipoproteins and inflammatory cells, leading to the development of complex lesions or plaques that protrude into the arterial lumen. Plaque rupture and thrombosis result in acute clinical complications such as myocardial infarction and stroke. Owing to the heterogeneous cellular composition of the plaques, a proteomic analysis of the whole lesion is not appropriate. Therefore, we have studied the proteins secreted by human carotid atherosclerotic plaques, obtained by endarterectomy. Normal artery segments and different regions of the surgical pieces (noncomplicated plaque, complicated plaque with thrombus) were cultured in protein-free medium and the secreted proteins (supernatants) analyzed by two-dimensional gel electrophoresis. Normal artery segments secreted a moderate number of proteins (42 spots). However in the two-dimensional (2-D) gels (pH 3-10) of segments bearing a plaque, the number of spots increased markedly (154). The number of spots also increased (202) in the 2-D gels of artery segments with a ruptured plaque and thrombus. Thus, the more complicated the lesion, the higher the number of secreted proteins, suggesting the production of specific proteins relating to the complexity of the atherosclerotic lesion.
authors.affiliation: Department Immunology, Fundación Jímenez Díaz, Madrid, Spain.
authors.forename: Mari Carmen
authors.initials: MC
authors.lastname: Duran
authors.forename: Sebastian
authors.initials: S
authors.lastname: Mas
authors.forename: Jose Luis
authors.initials: JL
authors.lastname: Martin-Ventura
authors.forename: Olivier
authors.initials: O
authors.lastname: Meilhac
authors.forename: Jean Baptiste
authors.initials: JB
authors.lastname: Michel
authors.forename: Julio
authors.initials: J
authors.lastname: Gallego-Delgado
authors.forename: Alberto
authors.initials: A
authors.lastname: Lázaro
authors.forename: José
authors.initials: J
authors.lastname: Tuñon
authors.forename: Jesus
authors.initials: J
authors.lastname: Egido
authors.forename: Fernando
authors.initials: F
authors.lastname: Vivanco
mesh_terms: D001161:Arteriosclerosis / Q000378:metabolism*; D001808:Blood Vessels / Q000737:chemistry*; D002339:Carotid Arteries / Q000737:chemistry; D002340:Carotid Artery Diseases / Q000473:pathology; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D016894:Endarterectomy, Carotid; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011506:Proteins / Q000378:metabolism; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics; D017534:Radial Artery / Q000737:chemistry
chemical_list: D011506:Proteins; D020543:Proteome