Electrospray ionization mass spectrometry coupled to liquid chromatography for detection of cisplatin and its hydrated complexes.
Electrospray ionization mass spectrometry (ESI-MS) coupled to liquid chromatography (LC) has been applied to investigate cisplatin and its hydrated complexes. Three hydrolysis products were identified following incubation of cisplatin in aqueous solution: monohydrated species, dihydrated species and the hydroxo-bridged dimer, each of which exhibited characteristic mass spectrometric behavior. The technique was employed to investigate the time- and pH-dependent hydrolysis of cisplatin in aqueous solution. The results have demonstrated that LC/ESI-MS is a powerful technique for the analysis of Pt(II) complexes and for monitoring their hydrolysis reactions.
Cui
Meng
M
Institute for National Measurement Standards, National Research Council Canada, Ontario, Canada, K1A 0R6.
Mester
Zoltán
Z
D000970:Antineoplastic Agents / Q000032:analysis*; D002853:Chromatography, Liquid; D002945:Cisplatin / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D012996:Solutions; D021241:Spectrometry, Mass, Electrospray Ionization
D000970:Antineoplastic Agents; D012996:Solutions; D002945:Cisplatin