title: T-cell receptor variable gamma chain gene expression in the interaction between rat gammadelta-type T cells and heat-shock protein 70-like molecule.
abstract: We previously reported that rat T-cell receptor (TCR) Vdelta6 of T-cell hybridomas was preferentially involved in recognition of the cell surface-expressed 70 kDa rat heat-shock cognate (hsc70, a constitutively expressed member of the hsp 70 family) protein-like molecule (#067 molecule). In the present study, we analyzed usage of the TCR Vgamma family of #067-restricted T-cell hybridomas. Our data indicated that most of these hybridomas expressed transcripts of TCR Vgamma1 and/or Vgamma2. However, some of the Vgamma2 transcripts were out-of-frame, suggesting that the TCR Vgamma1 family may be important for the recognition of #067-defined molecules. TCR Vgamma1 transcripts were detected in not only #067-restricted T-cell hybridomas, but #067-non restricted ones as well. However, V-J nucleotide sequences of #067-restricted and #067-non restricted T-cell hybridomas suggested that #067-restricted T-cell hybridomas showed limited insertion of nucleotide stretch as compared with #067-non restricted ones. In terms of amino acids, only one amino acid was added in #067-restricted T-cell hybridomas, whereas two or three amino acids were added in #067-non restricted ones. These data suggest that the heterodimer of the TCR relatively short stretch form of Vgamma1 molecule and TCR Vdelta6 may participate in recognition of the #067 molecule.
authors.affiliation: Department of Pathology, Cancer Research Institute, Sapporo Medical University School of Medicine, Sapporo, Hokkaido, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Ichinohe
authors.forename: Shingo
authors.initials: S
authors.lastname: Ichimiya
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kishi
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Tamura
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Kondo
authors.forename: Gosei
authors.initials: G
authors.lastname: Ueda
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Torigoe
authors.forename: Akira
authors.initials: A
authors.lastname: Yamaguchi
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Hiratsuka
authors.forename: Itaru
authors.initials: I
authors.lastname: Hirai
authors.forename: Genn-iku
authors.initials: GI
authors.lastname: Kohama
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Sato
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D022801:Complementarity Determining Regions; D005260:Female; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D019675:Genes, T-Cell Receptor gamma / Q000235:genetics / Q000502:physiology*; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D006825:Hybridomas; D051381:Rats; D011916:Rats, Inbred F344; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D022801:Complementarity Determining Regions; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; C494373:Hspa8 protein, rat; D016692:Receptors, Antigen, T-Cell, gamma-delta