Directed evolution of the substrate specificities of a site-specific recombinase and an aminoacyl-tRNA synthetase using fluorescence-activated cell sorting (FACS).
Santoro
Stephen W
SW
Department of Chemistry and the Skaggs Instutute for Chemical Biology, The Scripps Research Institute, La Jolla, CA, USA.
Schultz
Peter G
PG
D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D003001:Cloning, Molecular; D004254:DNA Nucleotidyltransferases / Q000235:genetics* / Q000378:metabolism*; D017931:DNA Primers; D019020:Directed Molecular Evolution / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics; D005434:Flow Cytometry / Q000379:methods; D017930:Genes, Reporter; D010957:Plasmids; D016133:Polymerase Chain Reaction; D045522:Recombinases; D015183:Restriction Mapping; D013379:Substrate Specificity
D017931:DNA Primers; D045522:Recombinases; D004254:DNA Nucleotidyltransferases; D000604:Amino Acyl-tRNA Synthetases