Yielding at stop codons: expanding the genetic code.
Codon-specific incorporation of noncoded amino acids into proteins can diversify the genetic code. Now, in both E. coli and S. cerevisiae, iterative rounds of selection can be used to isolate aminoacyl-tRNA synthetases that aminoacylate suppressor tRNAs with noncoded amino acids.
Hendrickson
Tamara L
TL
Johns Hopkins University, Department of Chemistry, 3400 N. Charles Street, Baltimore, MD 21218, USA.
D000596:Amino Acids / Q000096:biosynthesis / Q000235:genetics; D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics* / Q000302:isolation & purification; D018388:Codon, Terminator / Q000737:chemistry / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005815:Genetic Code; D016297:Mutagenesis, Site-Directed; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013379:Substrate Specificity / Q000235:genetics
D000596:Amino Acids; D018388:Codon, Terminator; D011506:Proteins; D000604:Amino Acyl-tRNA Synthetases