title: Screening of genes specifically activated in the pancreatic juice ductal cells from the patients with pancreatic ductal carcinoma.
abstract: Pancreatic ductal carcinoma (PDC) is one of the most intractable human malignancies. Surgical resection of PDC at curable stages is hampered by a lack of sensitive and reliable detection methods. Given that DNA microarray analysis allows the expression of thousands of genes to be monitored simultaneously, it offers a potentially suitable approach to the identification of molecular markers for the clinical diagnosis of PDC. However, a simple comparison between the transcriptomes of normal and cancerous pancreatic tissue is likely to yield misleading pseudopositive data that reflect mainly the different cellular compositions of the specimens. Indeed, a microarray comparison of normal and cancerous tissue identified the INSULIN gene as one of the genes whose expression was most specific to normal tissue. To eliminate such a "population-shift" effect, the pancreatic ductal epithelial cells were purified by MUC1-based affinity chromatography from pancreatic juice isolated from both healthy individuals and PDC patients. Analysis of these background-matched samples with DNA microarrays representing 3456 human genes resulted in the identification of candidate genes for PDC-specific markers, including those for AC133 and carcinoembryonic antigen-related cell adhesion molecule 7 (CEACAM7). Specific expression of these genes in the ductal cells of the patients with PDC was confirmed by quantitative real-time polymerase chain reaction analysis. Microarray analysis with purified pancreatic ductal cells has thus provided a basis for the development of a sensitive method for the detection of PDC that relies on pancreatic juice, which is routinely obtained in the clinical setting.
authors.affiliation: Division of Functional Genomics, Jichi Medical School, Kawachi-gun, Tochigi.
authors.forename: Koji
authors.initials: K
authors.lastname: Yoshida
authors.forename: Shuichi
authors.initials: S
authors.lastname: Ueno
authors.forename: Toshiyasu
authors.initials: T
authors.lastname: Iwao
authors.forename: Souichirou
authors.initials: S
authors.lastname: Yamasaki
authors.forename: Akira
authors.initials: A
authors.lastname: Tsuchida
authors.forename: Ken
authors.initials: K
authors.lastname: Ohmine
authors.forename: Ruri
authors.initials: R
authors.lastname: Ohki
authors.forename: Young Lim
authors.initials: YL
authors.lastname: Choi
authors.forename: Koji
authors.initials: K
authors.lastname: Koinuma
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Wada
authors.forename: Jun
authors.initials: J
authors.lastname: Ota
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Yamashita
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Chayama
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Sato
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Mano
mesh_terms: D001483:Base Sequence; D044584:Carcinoma, Ductal / Q000000981:diagnostic imaging / Q000235:genetics* / Q000473:pathology; D002760:Cholangiopancreatography, Endoscopic Retrograde; D017931:DNA Primers; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D005810:Multigene Family; D020411:Oligonucleotide Array Sequence Analysis; D010183:Pancreatic Ducts / Q000473:pathology*; D010189:Pancreatic Juice / Q000378:metabolism*; D010190:Pancreatic Neoplasms / Q000000981:diagnostic imaging / Q000235:genetics* / Q000473:pathology; D016133:Polymerase Chain Reaction; D012016:Reference Values
chemical_list: D017931:DNA Primers