Chemiluminescence determination of melatonin and some of its derivatives using potassium permanganate and formaldehyde system.
It was found that melatonin and its derivatives, such as N-acetyl- 5-methoxytryptamine (MT), N-acetyl-5-hydroxytryptamine (NAS), 5-Methoxytrypt- amine (5-MT), 5-Methoxyindolyl acetic acid (5-MIAA) and N-acetyl-5-methoxy- 6-hydroxytryptamine (6-HMT) would give chemiluminescence in the acidic potassium permanganate solution, and formaldehyde would enhance this chemiluminescent reaction greatly. The optimum conditions for this chemiluminescent reaction were studied in detail by a flow injection system. A new simple rapid method has been developed under the optimum conditions for determination of melatonin. This method has the advantages of high sensitivity, wide range of linear response and low detection limit. On the basis of investigation of chemiluminescent, fluorescent and UV spectra of melatonin in acidic solution containing potassium permanganate and formaldehyde, a possible mechanism of this reaction was proposed.
Chen
Guo Nan
GN
Department of Chemistry, Fuzhou University, 350002, Fuzhou, Fujian, China. gnchen@fzu.edu.cn
Huang
Fu Xin
FX
Wu
Xiao Ping
XP
Zhao
Zheng Feng
ZF
Duan
Jian Ping
JP
D017022:Flow Injection Analysis; D005557:Formaldehyde / Q000737:chemistry*; D007700:Kinetics; D008163:Luminescent Measurements; D008550:Melatonin / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry; D015394:Molecular Structure; D011196:Potassium Permanganate / Q000737:chemistry*
D011196:Potassium Permanganate; D005557:Formaldehyde; D008550:Melatonin