A PCR-based marker system monitoring CMS-(S), CMS-(T) and (N)-cytoplasm in the onion ( Allium cepa L.).
The chimerical mitochondrial CMS(1)-specific sequence in chives ( Allium schoenoprasum) was used to develop a PCR-marker that distinguishes both male-sterility inducing cytoplasms, CMS-(S) and CMS-(T), from the normal cytoplasm in onion ( Allium cepa). In combination with a previously described marker for CMS-(S), which anchors in the upstream region of the mitochondrial gene cob, all of the three known cytoplasms in the onion are distinguishable. The PCR-marker system was tested in 361 onion plants, which were selected from F(1)-hybrids and different open-pollinated varieties. The latter are mainly landraces from Turkey, in which all three cytoplasm types were detected.
Engelke
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Abteilung Angewandte Genetik, Universität Hannover, Herrenhäuser Strasse 2, D-30419 Hannover, Germany. thomas.engelke@genetik.uni-hannover.de
Terefe
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Tatlioglu
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D001947:Breeding; D003593:Cytoplasm / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D005298:Fertility / Q000235:genetics*; D017343:Genes, Plant / Q000502:physiology*; D005819:Genetic Markers; D005838:Genotype; D019697:Onions / Q000235:genetics*; D016366:Open Reading Frames / Q000502:physiology; D011058:Pollen / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods
D004272:DNA, Mitochondrial; D018744:DNA, Plant; D005819:Genetic Markers