X-ray analyses of DNA dodecamers containing 2'-deoxy-5-formyluridine.
It is known that formylation of thymine base induces purine transition in DNA replication. In order to establish the structural basis for such mutagenesis, crystal structures of two kinds of DNA dodecamers d(CGCGRATf5UCGCG) with f5U = 2'-deoxy-5-formyluridine and R = A or G have been determined. The f5U residues form a Watson-Crick-type pair with A and two types of pairs (wobble and reversed wobble) with G, the latter being the first example. Structural modeling suggests that the DNA polymerase can accept the reversed wobble pair with G, as well as the Watson-Crick pair with A.
Tsunoda
M
M
College of Science and Engineering, Iwaki Meisei University, Chuodai, Iwaki 970-8551, Japan.
Sakaue
T
T
Naito
S
S
Sunami
T
T
Karino
N
N
Ueno
Y
Y
Matsuda
A
A
Takénaka
A
A
D020029:Base Pairing; D001483:Base Sequence; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D003857:Deoxyuridine / Q000031:analogs & derivatives* / Q000737:chemistry*; D006860:Hydrogen Bonding; D008958:Models, Molecular
C061519:5-formyl-2'-deoxyuridine; D004247:DNA; D004259:DNA-Directed DNA Polymerase; D003857:Deoxyuridine