title: Escherichia coli can be transformed by a liposome-mediated lipofection method.
abstract: Transformation of Escherichia coli is a basic technique for genetic engineering. We used a liposome-mediated lipofection method to transform electrocompetent E. coli cells which has little natural competence of foreign DNA without electroporation treatment, and got transformants with simple and quick treatment by a plasmid or a transposon and transposase complex.
authors.affiliation: Special Division for Green Life Technology, National Institute of Advanced Industrial Science and Technology, 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan. y-kawata@aist.go.jp
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Kawata
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Yano
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kojima
mesh_terms: D004251:DNA Transposable Elements / Q000235:genetics; D018274:Electroporation; D004926:Escherichia coli / Q000235:genetics*; D018014:Gene Transfer Techniques; D008081:Liposomes; D010957:Plasmids / Q000235:genetics; D014169:Transformation, Bacterial / Q000235:genetics*; D019895:Transposases / Q000737:chemistry / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements; D008081:Liposomes; D019895:Transposases