Cutting edge: class I presentation of host peptides following HIV infection.
Class I MHC molecules bind intracellular peptides for presentation to cytotoxic T lymphocytes. Identification of peptides presented by class I molecules during infection is therefore a priority for detecting and targeting intracellular pathogens. To understand which host-encoded peptides distinguish HIV-infected cells, we have developed a mass spectrometric approach to characterize HLA-B*0702 peptides unique to or up-regulated on infected T cells. In this study, we identify 15 host proteins that are differentially presented on infected human T cells. Peptides with increased expression on HIV-infected cells were derived from multiple categories of cellular proteins including RNA binding proteins and cell cycle regulatory proteins. Therefore, comprehensive analysis of the B*0702 peptide repertoire demonstrates that marked differences in host protein presentation occur after HIV infection.
Hickman
Heather D
HD
Department of Microbiology and Immunology and William K. Warren Medical Research Institute, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
Luis
Angela D
AD
Bardet
Wilfried
W
Buchli
Rico
R
Battson
Casey L
CL
Shearer
Michael H
MH
Jackson
Kenneth W
KW
Kennedy
Ronald C
RC
Hildebrand
William H
WH
D017951:Antigen Presentation / Q000276:immunology*; D006678:HIV / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology / Q000378:metabolism / Q000821:virology; D015235:HLA-B Antigens / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D013058:Mass Spectrometry; D010446:Peptide Fragments / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D010449:Peptide Mapping; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism* / Q000821:virology*; D014162:Transfection; D014407:Tumor Cells, Cultured
D015235:HLA-B Antigens; D010446:Peptide Fragments