[Protein transduction by poly-arginine].
Protein transduction methods have been developed utilizing the delivery of peptides and proteins into eukaryotic cells by the protein transduction domain (PTD). Initially, the PTD domain was developed from the sequences from HIV-1 TAT, HSV VP-22 and antennapedia homeoprotein. Recently, several novel PTDs were developed and has been used as a valuable strategy for transduction of therapeutic protein. We developed a novel, high efficiency PTD (11 arginine) based on the TAT sequence and used 11R for the regulation of intracellular signal cascades. PTD can deliver proteins and other bioactive compounds and therefore serves as a very useful strategy for the development of therapeutic agents.
Matsui
Hideki
H
Department of Physiology, Okayama University, Graduate School of Medicine and Dentistry, Japan. matsuihi@cc.okayama-u.ac.jp
Tomizawa
Kazuhito
K
Matsushita
Masayuki
M
D000595:Amino Acid Sequence; D000818:Animals; D002467:Cell Nucleus; D002478:Cells, Cultured; D005057:Eukaryotic Cells; D010455:Peptides; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins; D015398:Signal Transduction; D014161:Transduction, Genetic / Q000379:methods*
D010455:Peptides; D011993:Recombinant Fusion Proteins; C015462:polyarginine