title: Chromosomal circularization in Streptomyces griseus by nonhomologous recombination of deletion ends.
abstract: Streptomyces linear chromosomes frequently cause deletions at both ends spontaneously or by various mutagenic treatments, and concomitantly display dynamic structural changes such as circularization and arm replacement. We have cloned and sequenced the fusion junctions of circularized chromosomes in two deletion mutants of Streptomyces griseus. No homology and a 1-bp overlap were found between the deletion ends of the mutant chromosomes. Taking this together with previous results, we concluded that chromosomal circularization in Streptomyces occurs by nonhomologous recombination between deletion ends.
authors.affiliation: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, Japan.
authors.forename: Shoichi
authors.initials: S
authors.lastname: Inoue
authors.forename: Koji
authors.initials: K
authors.lastname: Higashiyama
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Uchida
authors.forename: Keiichiro
authors.initials: K
authors.lastname: Hiratsu
authors.forename: Haruyasu
authors.initials: H
authors.lastname: Kinashi
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002872:Chromosome Deletion; D015825:Chromosomes, Fungal / Q000235:genetics*; D003470:Culture Media; D004271:DNA, Fungal / Q000096:biosynthesis; D016521:Electrophoresis, Gel, Pulsed-Field; D006824:Hybridization, Genetic; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D011995:Recombination, Genetic; D013056:Spectrophotometry, Ultraviolet; D013305:Streptomyces griseus / Q000235:genetics*
chemical_list: D003470:Culture Media; D004271:DNA, Fungal