title: Intestinal ischemia-reperfusion impairs liver regeneration after partial hepatectomy in rats.
abstract: OBJECTIVE
  The deleterious effects of intestinal ischemia-reperfusion on liver are realized, but its effect on the regenerative capacity of the liver has not been studied. Our aim in this study was to determine the effect of intestinal ischemia-reperfusion on liver regeneration.
  
  
  METHODS
  Sprague-Dawley rats were randomly divided into six groups; two sham-operated, two hepatectomy, and two hepatectomy with intestinal ischemia-reperfusion groups. To create intestinal ischemia-reperfusion, the superior mesenteric artery and collateral arteries supplying the small intestine were occluded for 20 minutes. Partial hepatectomy was performed during the period of ischemia. Ischemia-reperfusion injury in the mucosal layer of the small intestine was scored in light microscopy. Liver regeneration parameters (proliferating cell nuclear antigen labeling index for hepatocytes and liver regeneration rate), and serum levels of aspartate aminotransferase and alanine aminotransferase were studied on day 1 or 4 after operation.
  
  
  RESULTS
  Mucosal injury score was high in the hepatectomy with intestinal ischemia-reperfusion groups. Liver regeneration rate and proliferating cell nuclear antigen labeling index were less in these groups than the hepatectomy groups on day 1 and 4. There were no differences in the serum levels of aspartate aminotransferase and alanine aminotransferase between hepatectomy and hepatectomy with intestinal ischemia-reperfusion groups. The mortality rate was higher in the hepatectomy with intestinal ischemia-reperfusion groups than the other groups.
  
  
  CONCLUSIONS
  Ischemia and reperfusion of the small intestine impaired liver regeneration with high mortality after partial hepatectomy in the rats.
authors.affiliation: Department of Surgery, Celal Bayar University, Faculty of Medicine, Manisa, Turkey.
authors.forename: Ali Emre
authors.initials: AE
authors.lastname: Atici
authors.forename: Yavuz
authors.initials: Y
authors.lastname: Kaya
authors.forename: Teoman
authors.initials: T
authors.lastname: Coskun
authors.forename: Erinc
authors.initials: E
authors.lastname: Aral
authors.forename: Ahmet
authors.initials: A
authors.lastname: Var
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D006498:Hepatectomy; D022781:Hepatocytes / Q000473:pathology; D007413:Intestinal Mucosa / Q000098:blood supply / Q000473:pathology; D007422:Intestines / Q000098:blood supply* / Q000473:pathology; D007511:Ischemia / Q000473:pathology*; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000473:pathology*
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase