title: A single-center experience with retrograde reperfusion in liver transplantation.
abstract: Poor graft function secondary to injury by ischemia and reperfusion remains a major problem with regard to morbidity and mortality in clinical liver transplantation (LTX). Up to one fifth of patients suffer from poor initial liver function due to severe damage to hepatocytes. This situation leads either to primary nonfunction described in approximately 6% of LTX or to slow recovery. We present a new method of reperfusion during LTX. From July 1998 to July 2002, 42 LTX in 39 recipients, (10 female, 52 years old (26-70) were performed. LTX was carried out in piggy-back technique. After completing the piggy-back anastomosis, the caval vein was declamped immediately, and retrograde low pressure reperfusion of the graft with low oxygenated venous blood was established. Portal anastomosis was performed using a running suture. In order to provide optimal retrograde liver perfusion, no clamping of the donor portal vein was done. After completing portal anastomosis, the recipient portal vein was declamped immediately. During arterial anastomosis, the transplanted liver was antegradely perfused via the portal vein. After completing hepatic artery anastomosis, declamping of the hepatic artery was done and arterial perfusion started. No backtable or in-situ-flushing except the described reperfusion technique was performed. Forty-two LTX in 39 recipients using piggy-back technique and retrograde reperfusion via the caval vein followed by antegrade reperfusion via the portal vein were performed; 38 out of 39 patients (97.44%) were alive and well at day 8 after LTX. One patient (2.56%) died of a pre-existing portal vein thrombosis on day 2 after LTX. Three patients had to undergo retransplantation for hepatic artery thrombosis (7.14%). Liver enzymes, bilirubine, prothrombine time and AT III on day 1, 3, 5 and 8 after LTX showed favourable values. Median aspartate aminotransferase (ASAT) was 219 U/l on day 1 after LTX. One-month survival rate was 95.23%, and 1-year survival rate 87.88%. Two patients died of liver-associated causes (5.12%). One patient died of a late hepatic artery thrombosis, and one more of rejection. No other severe case of rejection appeared. We can conclude that retrograde reperfusion might be highly sufficient method of removing perfusion fluid from the transplanted liver. Low pressure perfusion with low oxygenated blood might reduce the production of free oxygen radicals. Retrograde reperfusion via the caval vein and antegrade reperfusion via the portal vein seemed to lower postoperative liver enzyme values and to improve initial liver function after LTX.
authors.affiliation: Department of Surgery, Division of Transplantation, Karl-Franzens University of Graz, Auenbruggerplatz 29, Postfach 89, 8036 Graz, Austria. daniela.kniepeiss@kfunigraz.ac.at
authors.forename: Daniela
authors.initials: D
authors.lastname: Kniepeiss
authors.forename: Florian
authors.initials: F
authors.lastname: Iberer
authors.forename: Barbara
authors.initials: B
authors.lastname: Grasser
authors.forename: Silvia
authors.initials: S
authors.lastname: Schaffellner
authors.forename: Vanessa
authors.initials: V
authors.lastname: Stadlbauer
authors.forename: Karl-Heinz
authors.initials: KH
authors.lastname: Tscheliessnigg
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D006085:Graft Survival; D006498:Hepatectomy / Q000379:methods; D022781:Hepatocytes / Q000166:cytology*; D006801:Humans; D008111:Liver Function Tests; D016031:Liver Transplantation / Q000379:methods* / Q000401:mortality / Q000473:pathology / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D011184:Postoperative Period; D015424:Reperfusion; D012189:Retrospective Studies; D015996:Survival Rate; D020858:Tissue and Organ Harvesting / Q000379:methods