Na(+)-dependent fructose transport via rNaGLT1 in rat kidney.
We found a system of Na(+)-dependent uptake of fructose by rat renal brush-border membrane vesicles. It consisted of two saturable components, and was thought to involve at least two transporters. rNaGLT1, a novel glucose transporter in rat kidney, showed fructose uptake as well as alpha-methyl-D-glucopyranoside uptake by transfected HEK293 cells. The features of the lower affinity type of fructose transporter in the brush-border membranes, such as affinity and substrate recognition, were very comparable with those of rNaGLT1-transfected HEK293 cells. These results indicated that rNaGLT1 is a primary fructose transporter in rat renal brush-border membranes.
Horiba
Naoshi
N
Department of Pharmacy, Kyoto University Hospital, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan.
Masuda
Satohiro
S
Ohnishi
Chiharu
C
Takeuchi
Daisuke
D
Okuda
Masahiro
M
Inui
Ken-ichi
K
D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D005632:Fructose / Q000378:metabolism*; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D008871:Microvilli / Q000378:metabolism; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D012964:Sodium / Q000378:metabolism*
D009004:Monosaccharide Transport Proteins; D011994:Recombinant Proteins; D005632:Fructose; D012964:Sodium