MGAlignIt: A web service for the alignment of mRNA/EST and genomic sequences.
Splicing is a biological phenomenon that removes the non-coding sequence from the transcripts to produce a mature transcript suitable for translation. To study this phenomenon, information on the intron-exon arrangement of a gene is essential, usually obtained by aligning mRNA/EST sequences to their cognate genomic sequences. MGAlign is a novel, rapid, memory efficient and practical method for aligning mRNA/EST and genome sequences. We present here a freely available web service, MGAlignIt (http://origin.bic.nus.edu.sg/mgalign/mgalignit), based on MGAlign. Besides the alignment itself, this web service allows users to effectively visualize the alignment in a graphical manner and to perform limited analysis on the alignment output. The server also permits the alignment to be saved in several forms, both graphical and text, suitable for further processing and analysis by other programs.
Lee
Bernett T K
BT
Department of Biochemistry, National University of Singapore, 8 Medical Drive, Singapore 117597, Singapore.
Tan
Tin Wee
TW
Ranganathan
Shoba
S
D000465:Algorithms; D003196:Computer Graphics; D005091:Exons; D020224:Expressed Sequence Tags; D015894:Genome, Human; D023281:Genomics / Q000379:methods; D006801:Humans; D020407:Internet; D007438:Introns; D012326:RNA Splicing; D012333:RNA, Messenger / Q000737:chemistry*; D016415:Sequence Alignment / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods; D017423:Sequence Analysis, RNA / Q000379:methods; D012984:Software
D012333:RNA, Messenger