Growth inhibition of the filamentous fungus Aspergillus nidulans by cadmium: an antioxidant enzyme approach.
The heavy metal cadmium is very toxic to biological systems. Although its effect on the growth of microorganisms and plants has been investigated, the response of antioxidant enzymes of Aspergillus nidulans to cadmium is not well documented. We have studied the effect of cadmium (supplied as CdCl(2)) on catalase (CAT), superoxide dismutase (SOD) and glutathione reductase (GR). 0.005 mM CdCl(2) had a very slight stimulatory effect on the growth rate of A. nidulans, but at concentrations above 0.025 mM, growth was totally inhibited. The accumulation of Cd within the mycelium was directly correlated with the increase in the concentration of CdC(2) used in the treatments. Although a cadmium-stimulated increase in SOD activity was observed, there was no change in the relative proportions of the individual Mn-SOD isoenzymes. Higher concentrations of CdCl(2) induced a small increase in total CAT activity, but there was a major increase in one isoenzymic form, that could be separated by gel electrophoresis. GR activity increased significantly following treatment with the highest concentration (0.05 mM) of CdCl(2). The increases in SOD, CAT, and GR activities suggest that CdCl(2) induces the formation of reactive oxygen species inside the mycelia of A. nidulans.
Guelfi
Andrea
A
Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de Sao Paulo, 13400-970, Piracicaba, Sao Paulo, Brazil.
Azevedo
Ricardo A
RA
Lea
Peter J
PJ
Molina
Silvia M G
SM
D001233:Aspergillus nidulans / Q000187:drug effects* / Q000201:enzymology* / Q000254:growth & development; D019256:Cadmium Chloride / Q000493:pharmacokinetics / Q000633:toxicity*; D002374:Catalase / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005980:Glutathione Reductase / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism; D025282:Mycelium / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D009195:Peroxidase / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism*
D007527:Isoenzymes; C121048:guaiacol peroxidase; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D019256:Cadmium Chloride