title: Synthesis of melanin-like pigments by Sporothrix schenckii in vitro and during mammalian infection.
abstract: Melanin has been implicated in the pathogenesis of several important human fungal pathogens. Existing data suggest that the conidia of the dimorphic fungal pathogen Sporothrix schenckii produce melanin or melanin-like compounds; in this study we aimed to confirm this suggestion and to demonstrate in vitro and in vivo production of melanin by yeast cells. S. schenckii grown on Mycosel agar produced visibly pigmented conidia, although yeast cells grown in brain heart infusion and minimal medium broth appeared to be nonpigmented macroscopically. However, treatment of both conidia and yeast cells with proteolytic enzymes, denaturant, and concentrated hot acid yielded dark particles similar in shape and size to the corresponding propagules, which were stable free radicals consistent with identification as melanins. Melanin particles extracted from S. schenckii yeast cells were used to produce a panel of murine monoclonal antibodies (MAbs) which labeled pigmented conidia, yeast cells, and the isolated particles. Tissue from hamster testicles infected with S. schenckii contained fungal cells that were labeled by melanin-binding MAbs, and digestion of infected hamster tissue yielded dark particles that were also reactive. Additionally, sera from humans with sporotrichosis contained antibodies that bound melanin particles. These findings indicate that S. schenckii conidia and yeast cells can produce melanin or melanin-like compounds in vitro and that yeast cells can synthesize pigment in vivo. Since melanin is an important virulence factor in other pathogenic fungi, this pigment may have a similar role in the pathogenesis of sporotrichosis.
authors.affiliation: Dermatology Department, St. John's Institute of Dermatology, Guy's Hospital, Guy's, Kings and St. Thomas' Medical Schools, London, United Kingdom. themojos@boltblue.com
authors.forename: Rachael
authors.initials: R
authors.lastname: Morris-Jones
authors.forename: Sirida
authors.initials: S
authors.lastname: Youngchim
authors.forename: Beatriz L
authors.initials: BL
authors.lastname: Gomez
authors.forename: Phil
authors.initials: P
authors.lastname: Aisen
authors.forename: Roderick J
authors.initials: RJ
authors.lastname: Hay
authors.forename: Joshua D
authors.initials: JD
authors.lastname: Nosanchuk
authors.forename: Arturo
authors.initials: A
authors.lastname: Casadevall
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Hamilton
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D004295:Dihydroxyphenylalanine / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008543:Melanins / Q000096:biosynthesis* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D013175:Sporothrix / Q000378:metabolism* / Q000472:pathogenicity; D013174:Sporotrichosis / Q000209:etiology* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D008543:Melanins; D004295:Dihydroxyphenylalanine