Correlation between VLA-4 integrin and hematopoietic cell migration.
To clarify the relationship between VLA-4 (CD49d) expression and hematopoietic cell migrating direction, mice were injected subcutaneously with diluted rhG-CSF for different times. The expressions of CD49d on Sca-1(+) cells were examined by flow cytometry. The relations between CD49d expression and Sca-1(+) cell enumerations were performed by statistical analysis. The results showed that with the administration of G-CSF, the expressions of CD49d in bone marrow (BM) and peripheral blood (PB) declined, meanwhile the number of Sca-1(+) cells in peripheral blood reached the peak in sharp contrast to BM nucleated cell number dropping on the seventh to ninth day. When CD49d expression rose again, the PB Sca-1(+) cells descended with the rising of BM nucleated cell number. In conclusion, VLA-4 mediates the hematopoietic cell adhesion to BM microenvironment. The regulation of CD49d expression may result in different migrating direction of hematopoietic cell between bone marrow and peripheral blood.
Chen
Tong
T
Department of Hematology, Huashan Hospital, Fudan University, Shanghai 200040, China. ctong@online.sh.cn
Xie
Yi
Y
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002452:Cell Count; D002465:Cell Movement / Q000187:drug effects; D005260:Female; D005434:Flow Cytometry; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D019650:Hematopoietic Stem Cell Mobilization; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D039041:Integrin alpha4beta1 / Q000097:blood*; D051379:Mice; D008807:Mice, Inbred BALB C; D013997:Time Factors
D039041:Integrin alpha4beta1; D016179:Granulocyte Colony-Stimulating Factor