Fully automated synthesis module for preparation of S-(2-[(18)F]fluoroethyl)-L-methionine by direct nucleophilic exchange on a quaternary 4-aminopyridinium resin.
A fully automated preparation of S-(2-[(18)F]fluoroethyl)-L-methionine (FEMET), an amino acid tracer for tumor imaging with positron emission tomography, is described. [(18)F]F(-) was produced via nuclear reaction (18)O(p,n) [(18)F] at PETtrace Cyclotron. Direction nucleophilic fluorination reaction of [(18)F]fluoride with 1,2-di(4-methylphenylsulfonyloxy)ethane on a quaternary 4-(4-methylpiperidinyl)pyridinium functionalized polystyrene anion exchange resin gave 2-[(18)F]-1-(4-methylphenyl-sulfonyloxy)ethane, and then [(18)F]fluoroalkylation of L-homocysteine thiolactone with 2-[(18)F]-1-(4-methylphenylsulfonyloxy)ethane yielded FEMET. The overall radiochemical yield with no decay correction was about 10%, the whole synthesis time was about 52 min, and the radiochemical purity was above 95%.
Tang
Ganghua
G
Nan Fang PET Centre, Nan Fang Hospital, The First Military Medical University, Guangzhou 510515, China. tghtgh@china.com
Wang
Mingfang
M
Tang
Xiaolan
X
Luo
Lei
L
Gan
Manquan
M
D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods*; D007475:Ion Exchange Resins; D007553:Isotope Labeling / Q000295:instrumentation / Q000379:methods*; D008715:Methionine / Q000031:analogs & derivatives / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification; D011743:Pyrimidines; D019275:Radiopharmaceuticals / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification; D011933:Reagent Kits, Diagnostic; D012371:Robotics / Q000295:instrumentation / Q000379:methods*; D018511:Systems Integration; D014055:Tomography, Emission-Computed / Q000295:instrumentation / Q000379:methods
D007475:Ion Exchange Resins; D011743:Pyrimidines; D019275:Radiopharmaceuticals; D011933:Reagent Kits, Diagnostic; C483764:S-(2-fluoroethyl)methionine; C034821:4-aminopyrimidine; D008715:Methionine