title: Synthesis of a fluorescent chemosensor suitable for the imaging of zinc(II) in live cells.
abstract: The synthesis of a coumarin-cyclen conjugate-based zinc-specific chemosensor and its ability to sense Zn(2+) in vitro is described. Using fluorescence microscopy, the chemosensor was shown to be capable of imaging Zn(2+) in live rat pituitary tumour cells.
authors.affiliation: University of Connecticut, Department of Chemistry, 06269-3060, Storrs, CT, USA.
authors.forename: Nathaniel C
authors.initials: NC
authors.lastname: Lim
authors.forename: Lili
authors.initials: L
authors.lastname: Yao
authors.forename: Hedley C
authors.initials: HC
authors.lastname: Freake
authors.forename: Christian
authors.initials: C
authors.lastname: Brückner
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002614:Chelating Agents / Q000138:chemical synthesis; D003374:Coumarins / Q000138:chemical synthesis; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D007202:Indicators and Reagents; D007700:Kinetics; D008856:Microscopy, Fluorescence; D010911:Pituitary Neoplasms / Q000378:metabolism / Q000473:pathology; D051381:Rats; D015032:Zinc / Q000378:metabolism*
chemical_list: D002614:Chelating Agents; D003374:Coumarins; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D015032:Zinc