[Simultaneous detection of HBV-DNA in hepatitis B surface antigen positive serum samples by "in-house" polymerase chain reaction and hybrid capture methods].
In this study, a total of 136 serum samples positive for hepatitis B surface antigen (HBsAg), have been screened for the presence of HBV DNA, by using in-house polymerase chain reaction (PCR) and hybrid capture (HC) assay (Digene Hybrid Capture System, UK), and the results have been compared by means of their performances. Of the serum samples tested, 53 (38.9%) and 34 (25%) were found positive for HBV DNA by PCR and HC assays, respectively. Accordingly, 19 (13.9%) PCR positive samples, were found negative with HC assay (p < 0.001). None of PCR negative samples were positive with HC assay. Eighty three (61%) of the 136 specimens were found negative by both of the methods. As a result, it was concluded that, in-house PCR was significantly more sensitive than HC assay for the detection of HBV DNA and may be used for the detection of HBV DNA, while HC assay may be useful especially for monitoring and predicting the response to antiviral therapy.
Us
Tercan
T
Osmangazi Universitesi Tip Fakültesi, Tibbi Mikrobiyoloji Anabilim Dali, Eskişehir.
Kiremitçi
Abdurrahman
A
Durmaz
Gül
G
Akgün
Yurdanur
Y
D004279:DNA, Viral / Q000097:blood*; D006509:Hepatitis B / Q000175:diagnosis*; D006514:Hepatitis B Surface Antigens / Q000097:blood*; D006515:Hepatitis B virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens