title: PBSC harvests individually optimized by using pre-collection CD34(+) values and on-line flow cytometric analysis of the mononuclear cell enrichment.
abstract: BACKGROUND
  We have investigated how the amount of blood processed during collection of PBSC affects the yield of CD34 cells.
  
  
  METHODS
  We first established a method of significantly increasing the enrichment of mononuclear cells (MNC), CD34(+) cells and granulocyte-macrophage progenitors (GM-CFU) by on-line flow cytometric (FCM) analysis of the leukocyte populations in the collect line. A total of 166 PBSC collections from 94 patients, were devided into five groups according to the blood volume processed: < 12 L of processed blood, 12-13L, 13-14L, 14-15L and > 15L.
  
  
  RESULTS
  When the yield of CD34(+) cells war compared between these groups, a positive correlation was seen (r=0.97) between the processed blood volume and the yield, expressed as a ratio between total number of CD34(+) cells in the harvest and the CD34(+) cell concentration in blood. This correlation can be used to estimate the volume that must be processed to exceed a specific target number of CD34(+) cells. The implications of these results on the need for one, two or more leukapheresis procedures in order to collect a sufficient amount of stem and progenitor cells for a given patient are discussed, i n relation to clinical logistics and the benefits for the patients.
  
  
  CONCLUSIONS
  PBSC harvest can be improved by individually-adjusted leukapheresis according to on-line FCM analysis and pre-harvest levels of CD34(+) cells and the processed blood volume can be used to predict the CD34(+) yield.
authors.affiliation: Department of Clinical Immunology and Transfusion Medicine, Karolinska Hospital, Stockholm Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Svensson
authors.forename: P
authors.initials: P
authors.lastname: Engervall
authors.forename: T
authors.initials: T
authors.lastname: Söderstrom
authors.forename: M
authors.initials: M
authors.lastname: Hansson
mesh_terms: D018952:Antigens, CD34 / Q000096:biosynthesis*; D001781:Blood Component Removal; D002452:Cell Count; D005434:Flow Cytometry / Q000379:methods*; D055014:Granulocyte-Macrophage Progenitor Cells; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D007937:Leukapheresis / Q000379:methods; D007962:Leukocytes / Q000166:cytology; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000378:metabolism; D009369:Neoplasms / Q000628:therapy*; D013234:Stem Cells / Q000166:cytology
chemical_list: D018952:Antigens, CD34