Two cases in which an abnormal hemoglobin (Hb Kamakura) was identified on the basis of an abnormally low glycohemoglobin levels.
We encountered two patients with abnormally low glycohemoglobin levels in spite of normal plasma glucose levels. Since the presence of an abnormal hemoglobin was suggested by high-performance liquid chromatography (HPLC), gene analysis of these two patients was conducted by single-strand conformation polymorphism (SSCP) analysis followed by direct sequencing, and Hb Kamakura with one base substitution (beta3 Leu --> Val) in the beta globin gene was detected in both patients. There was been only one report of Hb Kamakura in Kanagawa Prefecture, and our patients represent the second and third cases, respectively.
Saisho
Yoshifumi
Y
Department of Internal Medicine, Hiratsuka City Hospital, Hiratsuka.
Saitoh
Fumitake
F
Tabata
Mitsuhisa
M
Imafuku
Toshio
T
Ogihara
Tohru
T
Issiki
Ikuko
I
Hattori
Yukio
Y
Yamashiro
Yasuhiro
Y
D000328:Adult; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis*; D006455:Hemoglobins, Abnormal / Q000032:analysis* / Q000235:genetics; D006801:Humans; D008297:Male; D017354:Point Mutation; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA
D006442:Glycated Hemoglobin; D006455:Hemoglobins, Abnormal