Cloning and expression of the isoflavone synthase gene (IFS-Tp) from Trifolium pratense.
Isoflavones are secondary metabolites found mainly in leguminous plants. Their synthesis from flavanones is catalyzed by isoflavone synthase (IFS). We have cloned a isoflavone synthase gene (IFS-Tp) from Trifolium pratense that encodes a predicted 525 amino acids protein, molecular weight 59 kDa, with strong homology to IFS's from other legumes. IFS-Tp was expressed in all the tissues examined, and addition of glutathione and UV irradiation enhanced its expression. Microsomes from yeast transformed with IFS-Tp were able to convert naringenin to genistein, indicating that IFS-Tp has isoflavone synthase activity.
Kim
Bong Gyu
BG
Department of Forest and Environmental Science, Bio/Molecular Informatics Center, KonKuk University, Seoul 143-701, Korea.
Kim
Song-Young
SY
Song
Hee Su
HS
Lee
Chan
C
Hur
Hor-Gil
HG
Kim
Su-Il
SI
Ahn
Joong-Hoon
JH
D001483:Base Sequence; D003001:Cloning, Molecular; D015870:Gene Expression / Q000187:drug effects / Q000528:radiation effects; D005978:Glutathione / Q000494:pharmacology; D008969:Molecular Sequence Data; D010105:Oxygenases / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D016415:Sequence Alignment; D013379:Substrate Specificity; D014162:Transfection; D029921:Trifolium / Q000235:genetics; D014466:Ultraviolet Rays
D010105:Oxygenases; C048489:isoflavone synthase; D005978:Glutathione