title: Limitations of in silico predictability of specificity of co-immobilised cytochromes P450 and mimics in food-bioprocessing.
abstract: Cytochromes P450 (EC 1.14.14.1) are mixed function oxidases (oxygenases) that can catalyse redox bioconversions of food components. Also, efficacious removal of undesirable components can be achieved using solid-support immobilised enzyme (IME) of a selection from 2700 isoforms of cytochromes P450 (CYP). Cytochromes P450 co-immobilised with other enzymes, or protein receptors, may be used to confer a secondary order of regio- or stereo-specificity of chiral bioconversion: these can be predictable in silico by utilisation of QSARs (quantitative structure/activity relationships).
authors.affiliation: Biochemistry Group, School of Biomedical & Life Sciences, University of Surrey, Guildford, GU2 7XH, UK.
authors.forename: Alan
authors.initials: A
authors.lastname: Wiseman
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002273:Carcinogens / Q000378:metabolism; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000378:metabolism*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D005421:Flavoring Agents / Q000378:metabolism; D005503:Food Additives / Q000378:metabolism; D005511:Food Handling / Q000379:methods*; D018716:Molecular Mimicry; D010084:Oxidation-Reduction; D021281:Quantitative Structure-Activity Relationship; D017382:Reactive Oxygen Species / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D002273:Carcinogens; D004800:Enzymes, Immobilized; D005421:Flavoring Agents; D005503:Food Additives; D017382:Reactive Oxygen Species; D003577:Cytochrome P-450 Enzyme System; D019363:Cytochrome P-450 CYP1A1