title: Purification, crystallization and X-ray analysis of Hibiscus chlorotic ringspot virus.
abstract: Hibiscus chlorotic ringspot virus (HCRSV), a Carmovirus, occurs worldwide and induces chlorotic ringspots on leaves, stunting and flower distortion in Hibiscus species, including kenaf. The HCRSV capsid has T = 3 icosahedral symmetry and contains 180 copies of the coat protein. A virus yield of 48-70 mg per 100 g of infected kenaf leaves was achieved with an improved purification scheme involving sucrose-cushion and sucrose density-gradient centrifugation. The virus was crystallized using PEG 8000 and 2,3-butanediol as co-precipitants. The crystals belonged to the cubic space group P23, with unit-cell parameter a = 392 A, and diffracted X-rays to at least 4.5 A resolution.
authors.affiliation: Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609, Singapore.
authors.forename: Kian-Chung
authors.initials: KC
authors.lastname: Lee
authors.forename: Daina
authors.initials: D
authors.lastname: Lim
authors.forename: Sek-Man
authors.initials: SM
authors.lastname: Wong
authors.forename: Terje
authors.initials: T
authors.lastname: Dokland
mesh_terms: D002072:Butylene Glycols / Q000737:chemistry; D019184:Carmovirus / Q000737:chemistry*; D002499:Centrifugation, Density Gradient; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D008854:Microscopy, Electron; D009991:Oscillometry; D018515:Plant Leaves / Q000821:virology; D011092:Polyethylene Glycols / Q000494:pharmacology; D014961:X-Ray Diffraction
chemical_list: D002072:Butylene Glycols; D011092:Polyethylene Glycols; C026978:2,3-butylene glycol