title: Selective interactions of human kin17 and RPA proteins with chromatin and the nuclear matrix in a DNA damage- and cell cycle-regulated manner.
abstract: Several proteins involved in DNA synthesis are part of the so-called 'replication factories' that are anchored on non-chromatin nuclear structures. We report here that human kin17, a nuclear stress-activated protein, associates with both chromatin and non-chromatin nuclear structures in a cell cycle- and DNA damage-dependent manner. After L-mimosine block and withdrawal we observed that kin17 protein was recruited in the nucleus during re-entry and progression through S phase. These results are consistent with a role of kin17 protein in DNA replication. About 50% of the total amount of kin17 protein was detected on nuclear structures and could not be released by detergents. Furthermore, the amount of kin17 protein greatly increased in both G(1)/S and S phase-arrested cells in fractions containing proteins anchored to nuclear structures. The detection of kin17 protein showed for the first time its preferential assembly within non-chromatin nuclear structures in G(1)/S and S phase-arrested cells, while the association with these structures was found to be less stable in the G(2)/M phase, as judged by fractionation of human cells and immunostaining. In asynchronous growing cells, kin17 protein interacted with both chromatin DNA and non-chromatin nuclear structures, while in S phase-arrested cells it interacted mostly with non-chromatin nuclear structures, as judged by DNase I treatment and in vivo UV cross-linking. In the presence of DNA damage in S phase cells, the distribution of kin17 protein became mainly associated with chromosomal DNA, as judged by limited formaldehyde cross-linking of living cells. The physical interaction of kin17 protein with components of the nuclear matrix was confirmed and visualized by indirect immunofluorescence and immunoelectron microscopy. Our results indicate that, during S phase, a fraction of the human kin17 protein preferentially associates with the nuclear matrix, a fundamentally non-chromatin higher order nuclear structure, and to chromatin DNA in the presence of DNA damage.
authors.affiliation: Commissariat à l'Energie Atomique, Direction des Sciences du Vivant, Laboratoire de Génétique de la Radiosensibilité, Département de Radiobiologie et de Radiopathologie, F-92265 Fontenay-aux-Roses, France. miccoli@dsvidf.cea.fr
authors.forename: Laurent
authors.initials: L
authors.lastname: Miccoli
authors.forename: Denis S F
authors.initials: DS
authors.lastname: Biard
authors.forename: Isabelle
authors.initials: I
authors.lastname: Frouin
authors.forename: Francis
authors.initials: F
authors.lastname: Harper
authors.forename: Giovanni
authors.initials: G
authors.lastname: Maga
authors.forename: Jaime F
authors.initials: JF
authors.lastname: Angulo
mesh_terms: D002453:Cell Cycle / Q000502:physiology*; D002843:Chromatin / Q000235:genetics / Q000378:metabolism*; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004249:DNA Damage; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D015151:Immunoblotting; D016253:Microscopy, Immunoelectron; D015530:Nuclear Matrix / Q000378:metabolism* / Q000648:ultrastructure; D009687:Nuclear Proteins; D011485:Protein Binding; D016601:RNA-Binding Proteins; D051716:Replication Protein A; D016196:S Phase / Q000502:physiology; D014407:Tumor Cells, Cultured
chemical_list: D002843:Chromatin; D004268:DNA-Binding Proteins; C489487:KIN protein, human; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; C497167:RPA1 protein, human; D051716:Replication Protein A; D004247:DNA