The surface receptor is involved in annexin I-stimulated insulin secretion in MIN6N8a cells.
This study investigated the effect of extracellular annexin I on regulating insulin secretion in MIN6N8a (an insulin secreting cell line) cells. The properties of annexin I receptor in MIN6N8a cells were also determined. Annexin I stimulated insulin release in MIN6N8a cells, regardless of the presence or absence of extracellular Ca(2+). Confocal microscopy revealed that annexin I bound to the surface of MIN6N8a cells. In addition, FACs analysis showed that annexin I bound to the surface of MIN6N8a cells in a dose-dependent manner. However, the annexin I-stimulated insulin secretion and the annexin I binding were abolished in MIN6N8a cells treated with proteases. Annexin I receptors were regenerated time-dependently. Furthermore, annexin I-stimulated insulin secretion was inhibited by cycloheximide but not by actinomycin D. These results showed that annexin I binds to the surface receptor in order to regulate the stimulation of insulin release in MIN6N8a cells.
Won
Jong Hak
JH
Department of Biological Sciences and Institute for Basic Sciences, Sungkyunkwan University, Suwon, South Korea.
Kang
Na-na
NN
Auh
Chung-Kyoon
CK
Park
Young Min
YM
D000818:Animals; D017305:Annexin A1 / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D002469:Cell Separation; D003513:Cycloheximide / Q000378:metabolism; D003609:Dactinomycin / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D051379:Mice; D011485:Protein Binding; D011500:Protein Synthesis Inhibitors / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism*
D017305:Annexin A1; D007328:Insulin; D011500:Protein Synthesis Inhibitors; D011956:Receptors, Cell Surface; D003609:Dactinomycin; D003513:Cycloheximide; D002118:Calcium