Congestive heart failure in copper-deficient mice.
Copper Deficiency (CuD) leads to hypertrophic cardiomyopathy in various experimental models. The morphological, electrophysiological, and molecular aspects of this hypertrophy have been under investigation for a long time. However the transition from compensated hypertrophy to decompensated heart failure has not been investigated in the study of CuD. We set out to investigate the contractile and hemodynamic parameters of the CuD mouse heart and to determine whether heart failure follows hypertrophy in the CuD heart. Dams of FVB mice were fed CuD or copper-adequate (CuA) diet starting from the third day post delivery and the weanling pups were fed the same diet for a total period of 5 weeks (pre- and postweanling). At week 4, the functional parameters of the heart were analyzed using a surgical technique for catheterizing the left ventricle. A significant decrease in left ventricle systolic pressure was observed with no significant change in heart rate, and more importantly contractility as measured by the maximal rate of left ventricular pressure rise (+dP/dt) and decline (-dP/dt) were significantly depressed in the CuD mice. However, left ventricle end diastolic pressure was elevated, and relaxation was impaired in the CuD animals; the duration of relaxation was prolonged. In addition to significant changes in the basal level of cardiac function, CuD hearts had a blunted response to the stimulation of the beta-adrenergic agonist isoproterenol. Furthermore, morphological analysis revealed increased collagen accumulation in the CuD hearts along with lipid deposition. This study shows that CuD leads to systolic and diastolic dysfunction in association with histopathological changes, which are indices commonly used to diagnose congestive heart failure.
Elsherif
Laila
L
Department of Pharmacology and Toxicology, University of Louisville, Louisville, Kentucky 40202, USA.
Ortines
Raymond V
RV
Saari
Jack T
JT
Kang
Y James
YJ
D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D001835:Body Weight; D002570:Ceruloplasmin / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D003300:Copper / Q000097:blood / Q000172:deficiency*; D005260:Female; D006321:Heart / Q000503:physiopathology; D006333:Heart Failure / Q000209:etiology* / Q000503:physiopathology; D006439:Hemodynamics / Q000502:physiology; D007545:Isoproterenol / Q000494:pharmacology; D050356:Lipid Metabolism; D051379:Mice; D009200:Myocardial Contraction / Q000502:physiology; D009206:Myocardium / Q000201:enzymology; D009929:Organ Size; D013482:Superoxide Dismutase / Q000378:metabolism; D016277:Ventricular Function, Left / Q000502:physiology
D000318:Adrenergic beta-Agonists; D003300:Copper; D003094:Collagen; D013482:Superoxide Dismutase; D002570:Ceruloplasmin; D007545:Isoproterenol