rRNA intergenic spacer regions for phylogenetic analysis of Rickettsia species.
Species of the genus Rickettsia are responsible for several human diseases, namely epidemic typhus, Rocky Mountain spotted fever, and tick-borne typhus transmitted by arthropod vectors. The rrl-rrf intergenic spacer region (rrl-rrf ITS) was sequenced for 12 Rickettsia strains, including R. typhi, 6 untested species, R. aeschlimannii, Bar29, R. helvetica, R. honei, R. massilae, and R. slovaca as well as 5 Portuguese isolates. Phylogenetic trees inferred from rrl-rrf spacer sequences using maximum-parsimony and distance methods provided largely congruent tree topologies, supported by significant bootstrap values, enabling the identification of five distinct rickettsiae clusters.
Vitorino
Liliana
L
Departamento de Biologia Vegetal/Centro de Genética e Biologia Molecular, Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, Portugal.
Zé-Zé
Líbia
L
Sousa
Ana
A
Bacellar
Fatima
F
Tenreiro
Rogério
R
D000818:Animals; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005843:Geography; D006801:Humans; D007438:Introns; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012281:Rickettsia / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012282:Rickettsia Infections / Q000145:classification
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S