In-situ visualisation of hyphal structure and arrangement in mycoprotein pastes.
A novel method to examine the morphology and structure of fungal hyphae in solid pastes used for the production of meat alternative product is presented. A sample of fermentation broth was fluorescently stained with Calcofluor White and added back to the broth, mixed and then a paste made using ultra-filtration. Fibre visualisation was by fluorescence microscopy and quantification by manual image analysis. This method enables the determination of fibre length and orientation within the paste. Imaging of the hypha fibre paste proved that its structure was 'isotropic', i.e. that fibres were randomly oriented in all directions. Processing of the paste altered the orientation of the fibres, the method was able to identify and quantify the changes in fibre position.
Miri
Taghi
T
Centre for Formulation Engineering, Chemical Engineering, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
Cox
Philip W
PW
Fryer
Peter J
PJ
D001557:Benzenesulfonates; D016764:Cell Polarity; D004044:Dietary Proteins; D005502:Food; D005516:Food Microbiology; D005656:Fungal Proteins / Q000648:ultrastructure*; D005670:Fusarium / Q000166:cytology; D025301:Hyphae / Q000166:cytology*; D007089:Image Enhancement / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods; D009824:Ointments; D013194:Staining and Labeling / Q000379:methods*
D001557:Benzenesulfonates; D004044:Dietary Proteins; D005656:Fungal Proteins; D009824:Ointments; C007061:C.I. Fluorescent Brightening Agent 28