Crystallization and preliminary structure determination of the C-terminal truncated domain of the S-layer protein SbsC.
The C-terminal truncated form of the S-layer protein SbsC from Geobacillus stearothermophilus, rSbsC(31-844), has been crystallized by the vapour-diffusion method using polyethylene glycol 6000 as a precipitating agent. The crystals diffract to 3 A resolution using synchrotron radiation and belong to space group P2(1), with unit-cell parameters a = 57.24, b = 98.91, c = 108.62 A, beta = 94.34 degrees. One molecule is present in the asymmetric unit, which corresponds to a solvent content of 65%. Native and heavy-atom derivative data have been collected. The Pt derivative yielded two high-occupancy sites per molecule.
Pavkov
Tea
T
Institute of Chemistry/Structural Biology, Karl-Franzens-University Graz, Heinrichstrasse 28, 8010 Graz, Austria.
Oberer
Monika
M
Egelseer
Eva M
EM
Sára
Margit
M
Sleytr
Uwe B
UB
Keller
Walter
W
D001426:Bacterial Proteins / Q000737:chemistry*; D001665:Binding Sites; D003460:Crystallization; D018360:Crystallography, X-Ray; D001411:Geobacillus stearothermophilus / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000737:chemistry*; D017020:Methanosarcina / Q000378:metabolism; D010984:Platinum / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D013696:Temperature; D014961:X-Ray Diffraction
D001426:Bacterial Proteins; D008562:Membrane Glycoproteins; C055764:S-layer proteins; D011092:Polyethylene Glycols; D010984:Platinum