Analysis of FA contents in individual lipid fractions from human placental tissue.
Data on FA contents in the human placenta are limited. Different methods have been used for the FA analysis, and only percentage results have been presented. We developed and evaluated a method for the determination of FA concentrations in placental tissue. Lipids were extracted from placental tissue with a chloroform/methanol mixture; and phospholipids (PL), nonesterified FA (NEFA), TG, and cholesterol esters (CE) were isolated by TLC. Individual lipid fractions were derivatized with methanolic hydrochloric acid, and the FAME were quantified by GC with FID. The CV of intra-assay (n = 8) of absolute concentrations were evaluated for FA showing a tissue content > 0.01 mg/g. CV ranges were 4.6-11.0% for PL, 6.4-9.3% for NEFA, 6.1-8.9% for TG, and 11.4-16.3% for CE. The relative FA composition across a term placenta indicated no differences between samples of central and peripheral locations of maternal and fetal site (CV 0.5-9.9%), whereas the absolute FA concentrations were only reproducible in the PL fraction (CV 7.0-12.8%). The method shows a reasonably high precision that is well suited for physiological and nutritional studies.
Klingler
M
M
Division of Metabolism and Nutrition, Kinderklinik and Kinderpoliklinik, Dr. von Hauner Children's Hospital, Ludwig-Maximilians-University of Munich, D-80337, Munich, Germany.
Demmelmair
H
H
Larque
E
E
Koletzko
B
B
D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D002623:Chemistry Techniques, Analytical / Q000379:methods; D002788:Cholesterol Esters / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis*; D005229:Fatty Acids, Monounsaturated / Q000032:analysis; D005230:Fatty Acids, Nonesterified / Q000737:chemistry; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D005260:Female; D006801:Humans; D008055:Lipids / Q000737:chemistry* / Q000302:isolation & purification; D010084:Oxidation-Reduction / Q000187:drug effects; D010743:Phospholipids / Q000737:chemistry; D010920:Placenta / Q000737:chemistry*; D011247:Pregnancy; D015203:Reproducibility of Results; D014280:Triglycerides / Q000737:chemistry
D002788:Cholesterol Esters; D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D005230:Fatty Acids, Nonesterified; D005231:Fatty Acids, Unsaturated; D008055:Lipids; D010743:Phospholipids; D014280:Triglycerides; D002084:Butylated Hydroxytoluene