Synthesis and DNA binding properties of terminally modified peptide nucleic acids.
PNAs with terminal modifications of varying structure and charge were synthesized and their binding to DNA was studied. A variation in thermal stability of 19. 8 degrees C has been observed between the least and the most stable PNA-DNA duplexes. The most stable duplex melts 7.7 degrees C higher than the duplex of the corresponding non-modified PNA and complementary DNA. It has been shown that sequence fidelity of the PNA conjugate having the highest DNA affinity is significantly better than that of non-modified PNA. The results obtained can be used for the design of PNA probes, whose binding to DNA is sequence independent.
Mokhir
Andriy
A
Anorganisch-Chemisches Institut, Karl-Ruprechts University of Heidelberg, Im Neuenheimer Feld 270, Heidelberg 69120, Germany.
Zohm
Burkhard
B
Fuessl
Andreas
A
Kraemer
Roland
R
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004247:DNA / Q000378:metabolism*; D007202:Indicators and Reagents; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis* / Q000378:metabolism*; D011485:Protein Binding; D013816:Thermodynamics
D007202:Indicators and Reagents; D020135:Peptide Nucleic Acids; D004247:DNA