Suppression of decidual cell response induced by dibutyltin dichloride in pseudopregnant rats: as a cause of early embryonic loss.
In our previous study, dibutyltin dichloride (DBTCl) caused preimplantation embryonic loss and postimplantation embryonic loss in rats following administration at 7.6 mg/kg and above on Days 0-3 and at 3.8 mg/kg and above on Days 4-7 of pregnancy, respectively. This study was designed to assess the effects of DBTCl on uterine function as a cause of early embryonic loss using pseudopregnant rats. DBTCl was given orally to pseudopregnant rats at 3.8, 7.6 or 15.2 mg/kg on pseudopregnant day (PPD) 0-3 or on PPD 4-7. The decidual cell response was induced by bilateral uterine scratch on PPD 4. The uterine weight on PPD 9 served as an index of uterine decidualization. Uterine weight and serum progesterone levels on PPD 9 were significantly decreased after administration of DBTCl at 7.6 mg/kg and above on PPD 0-3 and PPD 4-7. DBTCl had no effect on the serum estradiol levels and number of corpora lutea. Administration of progesterone reversed the suppression of uterine decidualization in rats given DBTCl on PPD 0-3. It can be concluded that DBTCl suppresses the uterine decidual cell response and decreases progesterone levels, and these effects are responsible for early embryonic loss due to DBTCl exposure.
Harazono
Akira
A
National Institute of Health Sciences, Osaka Branch, 1-1-43 Hoenzaka, Chuo-ku, 540-0006 Osaka, Japan. harazono@nihs.go.jp
Ema
Makoto
M
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D003656:Decidua / Q000187:drug effects* / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D004435:Eating / Q000187:drug effects; D020964:Embryo Loss / Q000139:chemically induced / Q000209:etiology*; D005260:Female; D005865:Gestational Age; D007441:Intubation, Gastrointestinal; D009929:Organ Size / Q000187:drug effects; D009947:Organotin Compounds / Q000008:administration & dosage / Q000633:toxicity*; D011374:Progesterone / Q000097:blood; D011555:Pseudopregnancy / Q000097:blood; D051381:Rats; D017208:Rats, Wistar
D009947:Organotin Compounds; D011374:Progesterone; C020735:dibutyldichlorotin