title: Automated collection of blood components: their storage and transfusion.
abstract: The performance of an automated device, the Gambro TRIMA, was evaluated for component production, and an in vivo assessment of the platelets was carried out. Red cell concentrates (RCCs), platelets and plasma were collected and stored according to standard blood bank procedures and evaluated for quality by in vitro measurements. Additionally, single-donor platelets (n=10) were transfused to thrombocytopenic patients after 5 days of storage. Platelet counts were measured after 1 h and the corrected count increment (CCI) was calculated. No significant changes were seen before or after procedure in donor haemoglobin, haematocrit, coagulation factors or platelet count. Return-line samples showed no increase in the level of plasma haemoglobin. Plasma haemoglobin and potassium increased following RBC storage, but there was no change in the red cell number. Platelet aggregation decreased from 52 to 11% (adenosine diphosphate) and the Kunicki morphology score dropped from 379 to 174. Little change was seen in the hypotonic shock response (69-63%) or in the percentage of CD62 expression (4.8-19.8) over time. The CCI averaged 28+/-26 x 10(3) microL(-1) in 10 patients 1 h after transfusion. The TRIMA machine collects RCCs, platelets and plasma in a variety of combinations in one session. For autologous collection, two units of RCC plus platelets can be collected at one time, reducing administrative and testing costs. The platelets have good in vivo recovery, as shown by the CCI values. An added advantage is that the TRIMA machine can be used in hospitals to generate components in times of shortage without the need for a component's laboratory.
authors.affiliation: Department of Pathology and Laboratory Medicine, Ottawa Hospital and Canadian Blood Services, Ottawa, Ontario, Canada. grock@ottawahospital.on.ca
authors.forename: G
authors.initials: G
authors.lastname: Rock
authors.forename: C
authors.initials: C
authors.lastname: Moltzan
authors.forename: A
authors.initials: A
authors.lastname: Alharbi
authors.forename: A
authors.initials: A
authors.lastname: Giulivi
authors.forename: D
authors.initials: D
authors.lastname: Palmer
authors.forename: J
authors.initials: J
authors.lastname: Bormanis
mesh_terms: D001331:Automation; D001772:Blood Cell Count; D001781:Blood Component Removal / Q000295:instrumentation* / Q000379:methods; D016913:Blood Component Transfusion; D001782:Blood Donors; D001792:Blood Platelets; D001793:Blood Preservation; D001831:Body Temperature; D004867:Equipment Design; D004912:Erythrocytes; D006400:Hematocrit; D006439:Hemodynamics; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D010949:Plasma; D013997:Time Factors
chemical_list: D006454:Hemoglobins