[Sequence analysis of the deletion and mutation in carboxy terminal region of the Epstein-Barr virus latent membrane protein 1 derived from nasopharyngeal carcinoma patients].
BACKGROUND
To study the deletion and mutation in carboxy terminal region of LMP1 gene derived from nasopharyngeal carcinoma (NPC) in Guangdong and Guangxi, the high risk areas of nasopharyngeal carcinoma in China.


METHODS
LMP1 gene carboxy terminal region was amplified from nasopharyngeal carcinoma tissues by PCR, and then cloned and sequenced.


RESULTS
Of the 20 cases, 17 were LMP1 positive. In all positive cases, only 1 case did not show deletion. Four positive cases were chosen for DNA sequencing, The rusult showed that all the four cases had mutation and the 30bp deletion.


CONCLUSIONS
High frequency of deletion and mutation in LMP1 gene of nasopharyngeal carcinoma tissues was found in Guangdong and Guangxi. Whether it related to the high incidence of NPC should be further studied.
Tang
Min-zhong
MZ
Department of Central Laboratory, The Red Cross Hospital, Wuzhou 543002, China.
Zheng
Yu-ming
YM
Guo
Xiu-chan
XC
Zhang
Yong-li
YL
Zeng
Yi
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D017353:Gene Deletion; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009303:Nasopharyngeal Neoplasms / Q000821:virology*; D017354:Point Mutation; D017422:Sequence Analysis, DNA; D014763:Viral Matrix Proteins / Q000235:genetics*
C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins