title: Establishment of a new canine cell line (CCT) originated from a cutaneous malignant histiocytosis.
abstract: A new canine cell line, named CCT, was established from the cutaneous malignant histiocytosis in a 4-year-old male Borzoi. CCT proliferated with loose adherence and doubling time was approximately 30 hr. When co-cultured with latex beads, CCT phagocytized beads vigorously. Lysozyme and vimentin were positive by immunostaining, and non-specific esterase and acid phosphatase were positive by cytochemical staining. These features indicated the cells had a histiocytic nature. Furthermore, by subcutaneous injection to nude mice CCT could successfully form tumors with the morphological and immunohistochemical features similar to the original tumor.
authors.affiliation: Department of Veterinary Pathology, Faculty of Agriculture, Gifu University, Gifu, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Sakai
authors.forename: Hiroko
authors.initials: H
authors.lastname: Nakano
authors.forename: Ryouji
authors.initials: R
authors.lastname: Yamaguchi
authors.forename: Kayoko
authors.initials: K
authors.lastname: Yonemaru
authors.forename: Tokuma
authors.initials: T
authors.lastname: Yanai
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Masegi
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line / Q000473:pathology*; D004285:Dogs; D015620:Histiocytic Disorders, Malignant / Q000473:pathology* / Q000662:veterinary*; D008297:Male; D012867:Skin / Q000473:pathology; D012878:Skin Neoplasms / Q000473:pathology* / Q000662:veterinary*