title: Induced reactive oxygen species improve enzyme production from Aspergillus niger cultivation.
abstract: Intracellular reactive oxygen species (iROS) induction by HOCl was used as a novel strategy to improve enzyme productivities in Aspergillus niger growing in a bioreactor. With induced iROS, the specific intracellular activities of alpha-amylase, protease, catalase, and glucose oxidase were increased by about 170%, 250%, 320%, and 260%, respectively. The optimum specific iROS level for achieving maximum cell concentration and enzyme production was about 15 mmol g cell-1. The type of iROS inducing the enzyme production was identified to be a derivative of the superoxide radical.
authors.affiliation: Biochemical Engineering Group, Department of Chemical Engineering and School of Biosciences and Bioengineering, Indian Institute of Technology, Bombay, Powai, Mumbai 400 076, India.
authors.forename: Susmita
authors.initials: S
authors.lastname: Sahoo
authors.forename: K Krishnamurthy
authors.initials: KK
authors.lastname: Rao
authors.forename: G K
authors.initials: GK
authors.lastname: Suraishkumar
mesh_terms: D001234:Aspergillus niger / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism* / Q000502:physiology; D019149:Bioreactors; D002374:Catalase / Q000096:biosynthesis; D002455:Cell Division / Q000187:drug effects; D010450:Endopeptidases / Q000096:biosynthesis; D004798:Enzymes / Q000096:biosynthesis*; D005949:Glucose Oxidase; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D006867:Hydrolases / Q000096:biosynthesis; D006997:Hypochlorous Acid / Q000494:pharmacology*; D007218:Industrial Microbiology / Q000379:methods; D010088:Oxidoreductases / Q000096:biosynthesis; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism; D000516:alpha-Amylases
chemical_list: D004798:Enzymes; D017382:Reactive Oxygen Species; D013481:Superoxides; D006997:Hypochlorous Acid; D006861:Hydrogen Peroxide; D010088:Oxidoreductases; D005949:Glucose Oxidase; D002374:Catalase; D006867:Hydrolases; D000516:alpha-Amylases; D010450:Endopeptidases