Production process monitoring by serial mapping of microbial carbon flux distributions using a novel sensor reactor approach: I--Sensor reactor system.
A novel Sensor Reactor technology is presented which permits 13C labeling experiments for metabolic flux analysis during large-scale, semi-industrial, (fed-) batch fermentation processes deriving a series of flux maps that document fermentation courses in detail. The small-scale Sensor Reactor can be inoculated within 1.50-1.20s via a special inoculation unit with an inoculation volume accuracy of 1.025+/-0.021 L. The large-scale production reactor (here: 300 L) and the Sensor Reactor were run in parallel master/slave modes to control the current pH, temperature, pressure and dissolved oxygen values as changing set points for the Sensor Reactor. Using an automated pulsing technology, glucose pulses of 5 g/L could be realized within 0.51 s. The similarity of fermentations in the Sensor Reactor with the production process was demonstrated by studying L-lysine production with C. glutamicum during multiple, 'simulated' labeling experiments each lasting 2.5h. 'Real' labeling experiments are presented in Part II.
El Massaoudi
M
M
Institute of Biotechnology, Research Center Juelich, Forschungszentrum Julich, 52425, Juelich, Germany
Spelthahn
J
J
Drysch
A
A
de Graaf
A
A
Takors
R
R
D019149:Bioreactors / Q000382:microbiology*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D002244:Carbon / Q000032:analysis / Q000378:metabolism*; D002247:Carbon Isotopes / Q000378:metabolism; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods*; D003198:Computer Simulation; D003352:Corynebacterium / Q000145:classification / Q000254:growth & development / Q000378:metabolism; D003947:Diagnostic Techniques, Radioisotope; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005240:Feasibility Studies; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods; D005947:Glucose / Q000378:metabolism; D007553:Isotope Labeling / Q000379:methods; D008239:Lysine / Q000096:biosynthesis; D008954:Models, Biological
D002247:Carbon Isotopes; D002244:Carbon; D005947:Glucose; D008239:Lysine