title: Comparative analysis of type III effector translocation by Yersinia pseudotuberculosis expressing native LcrV or PcrV from Pseudomonas aeruginosa.
abstract: The homologues LcrV of Yersinia species and PcrV of Pseudomonas aeruginosa are pore-forming components. When expressed in a Yersinia lcrV background, PcrV formed smaller pores in infected erythrocyte membranes, correlating to a lowered translocation of Yersinia effectors. To understand this phenomenon, cytotoxins exoenzyme S of P. aeruginosa and YopE of Yersinia were introduced into a Yersinia background without Yop effectors but expressing LcrV or PcrV. Comparable translocation of each substrate indicated that substrate recognition by LcrV/PcrV is not a regulator of translocation. Yersinia harboring pcrV coexpressed with its native operon efficiently translocated effectors into HeLa cell monolayers and formed large LcrV-like pores in erythrocyte membranes. Thus, a PcrV complex with native P. aeruginosa translocon components is required to form fully functional pores for complete complementation of effector translocation in Yersinia.
authors.affiliation: Department of Molecular Biology, Umeå University, Umeå, Sweden.
authors.forename: Jeanette E
authors.initials: JE
authors.lastname: Bröms
authors.forename: Charlotta
authors.initials: C
authors.lastname: Sundin
authors.forename: Matthew S
authors.initials: MS
authors.lastname: Francis
authors.forename: Ake
authors.initials: A
authors.lastname: Forsberg
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics / Q000378:metabolism*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism; D001427:Bacterial Toxins / Q000235:genetics / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000378:metabolism / Q000382:microbiology; D017353:Gene Deletion; D015870:Gene Expression; D005816:Genetic Complementation Test; D006367:HeLa Cells; D000071677:Histidine Kinase; D006801:Humans; D009876:Operon / Q000235:genetics; D010641:Phenotype; D052899:Pore Forming Cytotoxic Proteins; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D013379:Substrate Specificity; D015011:Yersinia pseudotuberculosis / Q000235:genetics* / Q000378:metabolism*
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001427:Bacterial Toxins; C043908:LcrV protein, Yersinia; D052899:Pore Forming Cytotoxic Proteins; C118546:antigen V, Pseudomonas; C063239:yopE protein, Yersinia; D011494:Protein Kinases; D000071677:Histidine Kinase