title: Induction of 1-cys peroxiredoxin expression by oxidative stress in lung epithelial cells.
abstract: 1-Cys peroxiredoxin (1-cysPrx), a member of the peroxiredoxin family that contains a single conserved cysteine residue, reduces a broad spectrum of hydroperoxides. We studied changes in 1-cysPrx expression in rat lungs and lung cell lines in response to oxidative stress due to hyperoxia, H2O2, or paraquat. After 60 h of hyperoxia (>95% O2), mRNA and protein levels of 1-cysPrx and peroxidase activity were significantly elevated in rat lungs by approximately 1.5- to 2-fold compared with the control (P < 0.05). A similar induction of 1-cysPrx was observed in mouse lungs following exposure to O2 for 63 or 72 h; enzyme induction in mouse lungs was similar for wild-type and glutathione peroxidase 1 gene-targeted mice. H2O2 and paraquat treatment induced 1-cysPrx gene expression in L2 cells. Enzyme induction was attenuated by pretreatment with Trolox or N-acetylcysteine. Actinomycin D treatment showed that stability of 1-cysPrx mRNA was not altered in the presence of H2O2 or paraquat, indicating that increased expression with oxidative stress is regulated at the transcriptional level. These data indicate that the antioxidant enzyme 1-cysPrx is induced in lung cells by oxidative stress.
authors.affiliation: Institute for Environmental Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6068, USA.
authors.forename: Han-Suk
authors.initials: HS
authors.lastname: Kim
authors.forename: Yefim
authors.initials: Y
authors.lastname: Manevich
authors.forename: Sheldon I
authors.initials: SI
authors.lastname: Feinstein
authors.forename: Jhang Ho
authors.initials: JH
authors.lastname: Pak
authors.forename: Ye Shih
authors.initials: YS
authors.lastname: Ho
authors.forename: Aron B
authors.initials: AB
authors.lastname: Fisher
mesh_terms: D000818:Animals; D017124:Conserved Sequence; D005979:Glutathione Peroxidase / Q000235:genetics; D006861:Hydrogen Peroxide / Q000378:metabolism; D008168:Lung / Q000201:enzymology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018384:Oxidative Stress / Q000235:genetics*; D010269:Paraquat / Q000494:pharmacology; D010544:Peroxidases / Q000235:genetics*; D054464:Peroxiredoxins; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D020545:Respiratory Mucosa / Q000201:enzymology*; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D012333:RNA, Messenger; D006861:Hydrogen Peroxide; D010544:Peroxidases; D054464:Peroxiredoxins; D005979:Glutathione Peroxidase; D010269:Paraquat