title: Minisequencing on functionalised self-assembled monolayer as a simple approach for single nucleotide polymorphism analysis of cattle.
abstract: We have developed a genetic barcode module, based on a parallel sorting facility of single nucleotide polymorphism for secure individual identification of cattle. Biotinylated allele-specific oligonucleotides were immobilized onto the predefined spots of streptavidin tethered self-assembled monolayers with long chain alkanethiols on biochips. The target DNAs for hybridization and subsequent on-chip minisequencing were produced by multiplex PCR method. After enzymatic extension, only the moiety-modified dideoxynucleotide triphosphate, when coupled to its complementary target sequence, could be detected by the corresponding antibody to the moiety in a specific and sensitive manner. The database SNPZoo was developed for storage of the sequence information consisting of cytosine/thymidine patterns This SNP chip system can further be used in the detection of any replaceable point mutations occurring in the human and animal genes.
authors.affiliation: Antarus LifeScience GmbH, Sedanstr. 14, D-89077 Ulm, Germany.
authors.forename: Weidong
authors.initials: W
authors.lastname: Du
authors.forename: Michael
authors.initials: M
authors.lastname: Cieplik
authors.forename: Gregor
authors.initials: G
authors.lastname: Durstewitz
authors.forename: Chandra Mohan
authors.initials: CM
authors.lastname: Sarkar
authors.forename: Margit
authors.initials: M
authors.lastname: Kruschina
authors.forename: Rudi
authors.initials: R
authors.lastname: Fries
authors.forename: Flavio Ramalho
authors.initials: FR
authors.lastname: Ortigao
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D030561:Databases, Nucleic Acid; D005821:Genetic Techniques; D005838:Genotype; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide
chemical_list: D004247:DNA