Detection of oncogenic human papillomavirus in sporadic retinoblastoma.
OBJECTIVE
To investigate the presence of human papillomavirus (HPV) DNA in tumour tissue from patients with unilateral retinoblastoma.


METHODS
Samples of paraffin-embedded tumour tissue from 43 children with unilateral retinoblastoma were collected to investigate the presence of HPV DNA using polymerase chain reaction (PCR) and dot blot hybridization.


RESULTS
Oncogenic HPV DNA types 16 and 35 were detected in 12 (27.9%) of 43 tumour specimens. A higher frequency of differentiated tumours (63.3%) was observed among the HPV-positive tumours.


CONCLUSIONS
Future studies are necessary to demonstrate an association between HPV and sporadic retinoblastoma.
Palazzi
M A
MA
Boldrini Children's Centre, Rua Décio de Almeida Filho 421, Campinas 13084-710, São Paulo, Brazil. palazzi@lexxa.com.br
Yunes
J A
JA
Cardinalli
I A
IA
Stangenhaus
G P
GP
Brandalise
S R
SR
Ferreira
S A
SA
Sobrinho
J S P
JS
Villa
L L
LL
D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis; D015353:Eye Enucleation; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D007223:Infant; D008297:Male; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction; D019572:Retinal Neoplasms / Q000473:pathology / Q000601:surgery / Q000821:virology*; D012175:Retinoblastoma / Q000473:pathology / Q000601:surgery / Q000821:virology*; D014764:Viral Proteins / Q000032:analysis
D004279:DNA, Viral; D014764:Viral Proteins