A new kind of carbohydrate array, its use for profiling antiglycan antibodies, and the discovery of a novel human cellulose-binding antibody.
In this study, we use a novel glycan array to analyze the glycan-binding antibody repertoire in a pool of affinity-purified IgG collected from a healthy human population. The glycan array used is based on mono- and oligosaccharides covalently linked to the surface via a long linker at their reducing ends. They are thus presented to the medium with a well-defined orientation and are accessible for specific binding by glycan-binding proteins, such as antibodies and lectins. A novel anticellulose antibody was detected that binds specifically to beta4-linked saccharides with a preference for glucopyranose over galactopyranose residues. We also found previously known antiglycan antibodies against mono- and oligosaccharides that are constituents of commonly occurring bacterial polysaccharides. We propose that this array can facilitate high-throughput screening of glycan-binding proteins and the search for biomarkers for personalized medicine.
Schwarz
Mikael
M
Glycominds Ltd., 1 Yodfat Street, Alon Bldg., Lod 71291, Israel. mikaels@glycominds.com
Spector
Larissa
L
Gargir
Ari
A
Shtevi
Avraham
A
Gortler
Monica
M
Altstock
Rom T
RT
Dukler
Avinoam A
AA
Dotan
Nir
N
D000918:Antibody Specificity; D001665:Binding Sites; D002482:Cellulose / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology* / Q000302:isolation & purification; D037102:Lectins / Q000737:chemistry / Q000378:metabolism; D015336:Molecular Probe Techniques; D015394:Molecular Structure; D009005:Monosaccharides / Q000378:metabolism; D009844:Oligosaccharides / Q000378:metabolism; D011134:Polysaccharides / Q000276:immunology*; D011485:Protein Binding; D015203:Reproducibility of Results
D007074:Immunoglobulin G; D037102:Lectins; D009005:Monosaccharides; D009844:Oligosaccharides; D011134:Polysaccharides; D002482:Cellulose