Faster genetic identification of medically important aspergilli by using gellan gum as gelling agent in mycological media.
Using gellan gum as a substitute for agar-agar in a mycological medium and sequencing of the ITS 1 and 2 regions resulted in an accurate identification of Aspergillus fumigatus, Aspergillus nidulans, Aspergillus terreus and Aspergillus ustus within 24 h of subculture.
Schmidt
Dirk
D
Institut für Medizinische Mikrobiologie, Universitätsklinikum Essen, Hufelandstrasse 55, D-45122 Essen, Germany.
Rath
Peter-Michael
PM
Institut für Medizinische Mikrobiologie, Universitätsklinikum Essen, Hufelandstrasse 55, D-45122 Essen, Germany.
D001230:Aspergillus / Q000145:classification* / Q000235:genetics*; D016384:Consensus Sequence; D003470:Culture Media / Q000737:chemistry*; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D016533:Mycological Typing Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction; D011135:Polysaccharides, Bacterial; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013997:Time Factors
D003470:Culture Media; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D011135:Polysaccharides, Bacterial; C048288:gellan gum