title: Cross-linked cell aggregates of Trigonopsis variabilis: D-amino acid oxidase catalyst for oxidation of cephalosporin C.
abstract: Trigonopsis variabilis CBS 4095 was treated with alkali (pH 11, 30 min), heated (65 degrees C, 60 s) and immobilized. Glutaraldehyde, polyethyleneimine and a cross-linking reagent formed by reaction of polyethyleneimine with glutaraldehyde were used for stabilization of D-amino acid oxidase in the cells, as well as for aggregation and binding of the cells. A specific activity of 82-98 U of D-amino acid oxidase per g dry mass was produced with a yield of about 20%. The half-life time of 142 repeated conversion cycles corresponds to a productivity of 130 kg cephalosporin C oxidized per kg catalyst dry mass.
authors.affiliation: Laboratory of Enzyme Technology, Institute of Microbiology, Academy of Sciences of the Czech Republic, 14220 Prague 4, Czech Republic. becka@biomed.cas.cz
authors.forename: Stanislav
authors.initials: S
authors.lastname: Becka
authors.forename: Frantisek
authors.initials: F
authors.lastname: Skrob
authors.forename: Kamila
authors.initials: K
authors.lastname: Plhácková
authors.forename: Petr
authors.initials: P
authors.lastname: Kujan
authors.forename: Pavel
authors.initials: P
authors.lastname: Holler
authors.forename: Pavel
authors.initials: P
authors.lastname: Kyslík
mesh_terms: D002384:Catalysis; D002448:Cell Adhesion / Q000502:physiology; D002449:Cell Aggregation / Q000502:physiology; D002511:Cephalosporins / Q000096:biosynthesis*; D003605:D-Amino-Acid Oxidase / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D004789:Enzyme Activation; D004795:Enzyme Stability; D004800:Enzymes, Immobilized; D011786:Quality Control; D004718:Saccharomycetales / Q000145:classification / Q000201:enzymology* / Q000502:physiology / Q000648:ultrastructure*; D013045:Species Specificity
chemical_list: D002511:Cephalosporins; D004800:Enzymes, Immobilized; C025163:cephalosporin C; D003605:D-Amino-Acid Oxidase