title: Unraveling thioredoxin-linked metabolic processes of cereal starchy endosperm using proteomics.
abstract: Application of a thiol-specific probe, monobromobimane, with proteomics and enzyme assays led to the identification of 23 thioredoxin targets in the starchy endosperm of mature wheat seeds (Triticum aestivum cv. Butte), almost all containing at least two conserved cysteines. The identified targets, 12 not known to be thioredoxin-linked, function in a spectrum of processes: metabolism (12 targets), protein storage (three), oxidative stress (three), protein degradation (two), protein assembly/folding (one) and unknown reactions (two). In addition to formulating metabolic pathways functional in the endosperm, the results suggest that thioredoxin acts in redox regulation throughout the life cycle of the seed.
authors.affiliation: Department of Plant and Microbial Biology, 111 Koshland Hall, University of California, Berkeley, CA 94720, USA.
authors.forename: Joshua H
authors.initials: JH
authors.lastname: Wong
authors.forename: Yves
authors.initials: Y
authors.lastname: Balmer
authors.forename: Nick
authors.initials: N
authors.lastname: Cai
authors.forename: Charlene K
authors.initials: CK
authors.lastname: Tanaka
authors.forename: William H
authors.initials: WH
authors.lastname: Vensel
authors.forename: William J
authors.initials: WJ
authors.lastname: Hurkman
authors.forename: Bob B
authors.initials: BB
authors.lastname: Buchanan
mesh_terms: D017124:Conserved Sequence; D003545:Cysteine; D018384:Oxidative Stress; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D018514:Plant Structures / Q000378:metabolism; D012639:Seeds / Q000378:metabolism*; D013879:Thioredoxins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D014908:Triticum / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D013879:Thioredoxins; D003545:Cysteine