title: [Primary study on the electrophoresis atlas of the PCR products of the internal transcribed spacerl regions of the rRNA gene from home-planted and wild Gentiana macrophylla, G. straminea].
abstract: OBJECTIVE
  To analyse the electrophoresis atlas of the PCR amplified products of the internal transcribed spacerl regions of the rRNA gene in DNA extracted from home-planted and wild Gentiana macrophylla, G. straminea in Gansu.
  
  
  METHODS
  With synthetic peculiar PCR primer, internal transcribed spacerl sequences of rRNA gene were amplified in DNA extracted from home-planted and wild Gentiana macrophylla, G. straminea. And then the electrophoretic atlas of nPCR amplified products on the agar sugar gel were analysed.
  
  
  RESULTS
  The electrophoresis atlas showed that the lengths of the internal transcribed spacerl regions of the rRNA gene in DNA from Gentiana macrophylla, G. straminea had the same region 360 bp. The length had enough genetic information to analyse base sequence.
  
  
  CONCLUSIONS
  The PCR amplified products electrophoretic atlas of internal transcribed spacerl regions of the rRNA gene in DNA from Gentiana macrophylla, G. straminea can be used as one of signs for identification on molecule level.
authors.affiliation: Gansu College of Traditional Chinese Medicine, Lanzhou 730000.
authors.forename: Keping
authors.initials: K
authors.lastname: Ji
authors.forename: Lisha
authors.initials: L
authors.lastname: Liu
authors.forename: Xiling
authors.initials: X
authors.lastname: Zhang
authors.forename: Lan
authors.initials: L
authors.lastname: Wang
mesh_terms: D018744:DNA, Plant / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D020459:Genes, rRNA; D030012:Gentiana / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D018517:Plant Roots / Q000235:genetics; D010946:Plants, Medicinal / Q000235:genetics*; D016133:Polymerase Chain Reaction; D013045:Species Specificity
chemical_list: D018744:DNA, Plant