A water-soluble azobenzene cross-linker for photocontrol of peptide conformation.
We have designed and synthesized a water-soluble, sulfonated version of an azobenzene-based thiol-reactive cross-linker that can be introduced into peptides and proteins and act as a conformational photoswitch. The sulfonated compound is shown to effect a similar degree of conformational control on a model peptide helix system, as its nonsulfonated counterpart but can be introduced without the need for any organic cosolvent. The sulfonated azobenzene cross-linker thus expands the range of proteins to which photocontrol can be applied.
Zhang
Zhihua
Z
Department of Chemistry, University of Toronto, 80 St. George St., Toronto M5S 3H6, Canada.
Burns
Darcy C
DC
Kumita
Janet R
JR
Smart
Oliver S
OS
Woolley
G Andrew
GA
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D001391:Azo Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D002942:Circular Dichroism; D003432:Cross-Linking Reagents / Q000737:chemistry*; D008956:Models, Chemical; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry*; D010777:Photochemistry; D011487:Protein Conformation; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D013816:Thermodynamics; D014867:Water / Q000737:chemistry*
D000596:Amino Acids; D001391:Azo Compounds; D003432:Cross-Linking Reagents; D010455:Peptides; D014867:Water