Ontogeny of gamma-aminobutyric acid-immunoreactive neurons in the rhombencephalon and spinal cord of the sea lamprey.
The development of neurons expressing gamma-aminobutyric acid (GABA) in the rhombencephalon and spinal cord of the sea lamprey (Petromyzon marinus) was studied for the first time with an anti-GABA antibody. The earliest GABA-immunoreactive (GABAir) neurons appear in late embryos in the basal plate of the isthmus, caudal rhombencephalon, and rostral spinal cord. In prolarvae, the GABAir neurons of the rhombencephalon appear to be distributed in spatially restricted cellular domains that, at the end of the prolarval period, form four longitudinal GABAir bands (alar dorsal, alar ventral, dorsal basal, and ventral basal). In the spinal cord, we observed only three GABAir longitudinal bands (dorsal, intermediate, and ventral). The larval pattern of GABAir neuronal populations was established by the 30-mm stage, and the same populations were observed in premetamorphic and adult lampreys. The ontogeny of GABAergic populations in the lamprey rhombencephalon and spinal cord is, in general, similar to that previously described in mouse and Xenopus.
Meléndez-Ferro
Miguel
M
Departamento de Biología Celular y Ecología, Facultad de Biología, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain.
Pérez-Costas
Emma
E
Villar-Cheda
Begoña
B
Rodríguez-Muñoz
Rolando
R
Anadón
Ramón
R
Rodicio
María Celina
MC
D000818:Animals; D000831:Animals, Newborn; D019521:Body Patterning; D002452:Cell Count; D048429:Cell Size; D004625:Embryo, Nonmammalian; D005314:Embryonic and Fetal Development / Q000502:physiology; D007150:Immunohistochemistry; D007798:Lampreys; D007814:Larva; D009412:Nerve Fibers; D009433:Neural Inhibition; D009474:Neurons / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D012249:Rhombencephalon / Q000166:cytology* / Q000196:embryology / Q000254:growth & development; D013116:Spinal Cord / Q000166:cytology* / Q000196:embryology / Q000254:growth & development; D013997:Time Factors; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D018809:Proliferating Cell Nuclear Antigen; D005680:gamma-Aminobutyric Acid