Physiology of a colony of Pleurotus pulmonarius grown on medium overlaid with a Cellophane membrane.
The physiology of a colony of Pleurotus pulmonarius grown on potato dextrose agar overlaid with a Cellophane membrane (PDA-WC) was studied. On PDA-WC, the colony presented higher biomass density and productivity of fruit body formation (4.84+/-0.23 mg mycelial biomass/cm2 and 23.7+/-3.12 microg ml(-1) h(-1), respectively) than that which developed on potato dextrose agar without Cellophane (PDA-OC; 0.26+/-0.01 mg mycelial biomass/cm2 and 10.8+/-1.57 microg ml(-1) h(-1), respectively). In cultures developed on PDA-WC, intracellular laccases and beta-1,3-glucanases activities were lower [12+/-0.9 arbitrary units (AU)/g mycelial biomass and 1.33+/-0.1 international units (IU)/g mycelial biomass, respectively] than those observed on PDA-OC (20.65+/-1.0 AU/g mycelial biomass and 3.67+/-0.2 IU/g mycelial biomass, respectively). In cultures developed on PDA-WC, intracellular protein and glycogen concentrations were lower (1.9+/-0.9 and 117+/-3.5 mg/g mycelial biomass, respectively) than those observed on PDA-OC (14.3+/-1.1 and 347+/-2.9 mg/g mycelial biomass, respectively). The radial growth rate and the content of glucans in the cell wall were not significantly different between cultures developed on PDA-WC and PDA-OC. These results show that the use of the Cellophane as a tool to study in vitro fungal physiology might affect the interpretation of experimental results, since the physiology under otherwise similar conditions was different on medium with and without Cellophane.
Téllez-Téllez
M
M
Laboratory of Biotechnology, Research Center for Biological Sciences, Universidad Autónoma de Tlaxcala, Apartado postal 129, C.P. 90000 Tlaxcala, Tlax, Mexico.
Díaz-Godínez
G
G
Sánchez
C
C
D018533:Biomass; D002476:Cellophane; D003470:Culture Media; D005656:Fungal Proteins / Q000378:metabolism; D005936:Glucans / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D008567:Membranes, Artificial; D009172:Mycology / Q000379:methods; D020076:Pleurotus / Q000201:enzymology / Q000254:growth & development* / Q000502:physiology*
D003470:Culture Media; D005656:Fungal Proteins; D005936:Glucans; D008567:Membranes, Artificial; D006003:Glycogen; D002476:Cellophane