Increased intensities of YOYO-1-labeled DNA oligomers near silver particles.
DNA detection is usually performed using fluorescence probes. Using a DNA oligomer stained with the widely used dye 1,1'-[1,3-propanediylbis[(dimethylimino)-3,1-propanediyl]]bis[4-[(3-methyl-2(3H)-benzoxazolylidene)methyl]]-quinolinum tetraiodide (YOYO-1), we show that a substrate containing silver particles can lead to a greater than 10-fold increase in the fluorescence intensity. Proximity to silver particles also increases the photostability of YOYO-1-DNA. These results suggest that substrates or gels containing silver particles may be used for increased sensitivity in DNA detection.
Lakowicz
Joseph R
JR
Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, School of Medicine, University of Maryland at Baltimore, Baltimore, MD 21201, USA. cfs@cfs.umbi.umd.edu
Malicka
Joanna
J
Gryczynski
Ignacy
I
D001483:Base Sequence; D001583:Benzoxazoles / Q000032:analysis* / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry; D010777:Photochemistry; D011806:Quinolinium Compounds / Q000032:analysis* / Q000737:chemistry; D012680:Sensitivity and Specificity; D012834:Silver; D013052:Spectrometry, X-Ray Emission
D001583:Benzoxazoles; D005456:Fluorescent Dyes; D011806:Quinolinium Compounds; C075296:1,1'-((4,4,7,7-tetramethyl)-4,7-diazaundecamethylene)bis-4-(3-methyl-2,3-dihydro(benzo-1,3-oxazole)-2-methylidene)quinolinium; D012834:Silver; D004247:DNA