title: [Analysis of CD117 expression on leukemia cells].
abstract: OBJECTIVE
  To explore the expression of CD(117) on different types of leukemia and its significance.
  
  
  METHODS
  CD(117) expression was analysed by three-color flow cytometry with CD(45)/SSC gating strategy.
  
  
  RESULTS
  CD(117) was expressed in 68% of acute myeloid leukemia (AML) and 80% of chronic myeloid leukemia in blast crisis (CML-BC) cases, but in only 2% of acute lymphoblastic leukemia cases. CD(117) was negative in CML in chronic phase and chronic lymphocytic leukemia. Cases in AML, CD(117) was expressed in 72% of M(0)/M(1), 88% of M(2), 50% of M(4), 75% of M(5a), 100% of M(6) and 100% of M(7) cases but was less expressed in M(3) (39%) and M(5b) (29%) cases. Expression of CD(117) was more frequent than CD(34) and HLA-DR in M(3) and some M(2) cases. There was a significant inverse relationship between CD(117) and CD(14) expression in AML.
  
  
  CONCLUSIONS
  Analysis of CD(117) expression may be of help in distinguishing myeloid from lymphoid leukemia and leukemia clone from normal cells.
authors.affiliation: Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
authors.forename: Hong-qiang
authors.initials: HQ
authors.lastname: Shen
authors.forename: Yong-min
authors.initials: YM
authors.lastname: Tang
authors.forename: Shi-long
authors.initials: SL
authors.lastname: Yang
authors.forename: Bo-qin
authors.initials: BQ
authors.lastname: Qian
authors.forename: Hua
authors.initials: H
authors.lastname: Song
authors.forename: Shu-wen
authors.initials: SW
authors.lastname: Shi
authors.forename: Wei-qun
authors.initials: WQ
authors.lastname: Xu
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D003937:Diagnosis, Differential; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007223:Infant; D007945:Leukemia, Lymphoid / Q000175:diagnosis / Q000378:metabolism*; D007951:Leukemia, Myeloid / Q000175:diagnosis / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D019009:Proto-Oncogene Proteins c-kit / Q000032:analysis / Q000096:biosynthesis*
chemical_list: D019009:Proto-Oncogene Proteins c-kit