Laser-induced fluorescence technique for DNA and proteins separated by capillary electrophoresis.
Recent developments in capillary electrophoresis (CE) in conjunction with laser-induced fluorescence (LIF) using long-wavelength (maximum excitation wavelength>500 nm) dyes are reviewed. These dyes are particularly of interest when conducting the analyses of biopolymers by CE-LIF using He-Ne lasers. These systems are benefited from low background, low costs, easy maintenance, and compactness. Derivatizations of DNA and proteins with fluorescent or nonfluorescent chemicals can be carried out prior to, during, or after separations. With the advantages of sensitivity, rapidity, and high efficiency, the applications of CE-LIF to the analysis of polymerase chain reaction products, DNA sequencing, trace analysis of proteins, and single cell analysis have been presented.
Lin
Yang-Wei
YW
Department of Chemistry, National Taiwan University, 1, Section 4, Roosevelt Road, Taipei, Taiwan.
Chiu
Tai-Chia
TC
Chang
Huan-Tsung
HT
D004247:DNA / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods*; D007364:Intercalating Agents / Q000737:chemistry; D007834:Lasers; D011506:Proteins / Q000302:isolation & purification*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D007364:Intercalating Agents; D011506:Proteins; D004247:DNA