Receptor use by vesicular stomatitis virus pseudotypes with glycoproteins of defective variants of measles virus isolated from brains of patients with subacute sclerosing panencephalitis.
The vaccine or Vero cell-adapted strains of measles virus (MV) have been reported to use CD46 as a cell entry receptor, while lymphotropic MVs preferentially use the signalling lymphocyte activation molecule (SLAM or CD150). In contrast to the virus obtained from patients with acute measles, little is known about the receptor that is used by defective variants of MV isolated from patients with subacute sclerosing panencephalitis (SSPE). The receptor-binding properties of SSPE strains of MV were analysed using vesicular stomatitis virus pseudotypes expressing the envelope glycoproteins of SSPE strains of MV. Such pseudotype viruses could use SLAM but not CD46 for entry. The pseudotype viruses with SSPE envelope glycoproteins could enter Vero cells, which do not express SLAM. In addition, their entry was not blocked by the monoclonal antibody to CD46, pointing to another entry receptor for SSPE strains on Vero cells. Furthermore, the unknown receptor(s), distinct from SLAM and CD46, may be present on cell lines derived from lymphoid and neural cells. Biochemical characterization of the receptor present on Vero cells and SK-N-SH neuroblastoma cells was consistent with a glycoprotein. Identification of additional entry receptors for MV will provide new insights into the mechanism of spread of MV in the central nervous system and possible reasons for differences between MVs isolated from patients with acute measles and SSPE.
Shingai
Masashi
M
Department of Virology, Osaka City University Medical School, Asahimachi, Abeno-ku, Osaka 545-8585, Japan.
Ayata
Minoru
M
Department of Virology, Osaka City University Medical School, Asahimachi, Abeno-ku, Osaka 545-8585, Japan.
Ishida
Hiroshi
H
Department of Pediatrics, Osaka City University Medical School, Asahimachi, Abeno-ku, Osaka 545-8585, Japan.|Department of Virology, Osaka City University Medical School, Asahimachi, Abeno-ku, Osaka 545-8585, Japan.
Matsunaga
Isamu
I
Department of Virology, Osaka City University Medical School, Asahimachi, Abeno-ku, Osaka 545-8585, Japan.
Katayama
Yuko
Y
Department of Virology, Osaka City University Medical School, Asahimachi, Abeno-ku, Osaka 545-8585, Japan.
Seya
Tsukasa
T
Department of Immunology, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka 537-8511, Japan.
Tatsuo
Hironobu
H
Department of Virology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
Yanagi
Yusuke
Y
Department of Virology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
Ogura
Hisashi
H
Department of Virology, Osaka City University Medical School, Asahimachi, Abeno-ku, Osaka 545-8585, Japan.
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001921:Brain / Q000821:virology; D002460:Cell Line; D006224:Cricetinae; D003673:Defective Viruses / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity; D014644:Genetic Variation; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D008459:Measles virus / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D011956:Receptors, Cell Surface; D011991:Receptors, Virus / Q000378:metabolism*; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1; D013344:Subacute Sclerosing Panencephalitis / Q000821:virology*; D014407:Tumor Cells, Cultured; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity
D015703:Antigens, CD; C497857:CD46 protein, human; D006023:Glycoproteins; D007136:Immunoglobulins; C497859:Mcp protein, mouse; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D011991:Receptors, Virus; C000604059:SLAMF1 protein, human; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1