Photodestruction intermediates probed by an adjacent reporter molecule.
We used a fluorescence resonance energy transfer donor molecule to probe the multiple intermediates in the photoinduced destruction of an acceptor molecule. These intermediates are nonemitting but are still able to quench the fluorescence of the donor at a distance scale shorter than conventional fluorescence resonance energy transfer, suggesting novel biophysical applications.
Ha
Taekjip
T
Department of Physics, University of Illinois at Urbana-Champaign, Urbana, Illinois, 61801, USA.
Xu
Jian
J
D001710:Biotin / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry
D005456:Fluorescent Dyes; D001710:Biotin; D004247:DNA