title: Brain expression of apurinic/apyrimidinic endonuclease (APE/Ref-1) multifunctional DNA repair enzyme gene in the mouse with special reference to the suprachiasmatic nucleus.
abstract: Multifunctional mammalian apurinic/apyrimidinic endonuclease (APE, also known as redox factor-1; Ref-1) repairs baseless sites of damaged DNA caused by oxidative stress and regulates the redox state of various DNA binding proteins. Here, we examined the expression of APE/Ref-1 m-RNA in the mouse brain by in situ hybridization. We detected APE/Ref-1 transcripts throughout the mouse brain particularly in the clock oscillating neurons of the suprachiasmatic nucleus (SCN), hippocampal pyramidal cells, granular cells, and in monoaminergic neurons. In the circadian center SCN, levels of APE/ref-1 mRNA transcripts were constantly high, and were not influenced by either circadian rhythms or by exposure to light.
authors.affiliation: Division of Molecular Brain Science, Department of Brain Sciences, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan.
authors.forename: Hidehito
authors.initials: H
authors.lastname: Kimura
authors.forename: Xing
authors.initials: X
authors.lastname: Dong
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Yagita
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Okamura
mesh_terms: D000818:Animals; D001345:Autoradiography / Q000379:methods; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism; D001931:Brain Mapping; D019757:Carbon-Oxygen Lyases / Q000235:genetics / Q000378:metabolism*; D002940:Circadian Rhythm / Q000502:physiology; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D015870:Gene Expression; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013116:Spinal Cord / Q000378:metabolism; D013493:Suprachiasmatic Nucleus / Q000378:metabolism*; D013997:Time Factors
chemical_list: D012333:RNA, Messenger; D019757:Carbon-Oxygen Lyases; C486216:Apex1 protein, mouse; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase