title: Comparison of ammoniated and nonammoniated extracts in children with latex allergy.
abstract: BACKGROUND
  The use of ammoniated or nonammoniated latex extracts for the diagnosis of latex allergy is still a matter of debate. The aim of our study was to compare the characteristics of the two types of extracts by immunoblotting and RAST techniques in children with ascertained latex allergy.
  
  
  METHODS
  Ammoniated (AL) and nonammoniated latex (NAL) extracts were prepared and blotted on SDS-PAGE to resolve their components. Also a solid phase for RAST assays was prepared with the two extracts. The sera from 18 children (mean age 11.4 years, range 6-15 years), with ascertained latex allergy (clinical history, skin test, CAP-RAST and provocation) were used for the experiments.
  
  
  RESULTS
  The NAL extract is resolved in many bands (5-100 kDa), whereas AL showed only few components, likely Hev b 4, 6 and 7. IgE reactivity against AL was observed only in 5/18 patients, whereas 12/18 were positive with NAL. The blotting profile against NAL was complex and the IgE recognition pattern involved different bands.
  
  
  CONCLUSIONS
  The extract obtained from NAL is able to detect specific IgE against a greater number of allergenic determinants, and therefore a greater diagnostic accuracy can be expected.
authors.affiliation: Allergy Unit, Children Hospital, ARNAS-Palermo, Palermo, Italy.
authors.forename: S
authors.initials: S
authors.lastname: La Grutta
authors.forename: G
authors.initials: G
authors.lastname: Mistrello
authors.forename: E
authors.initials: E
authors.lastname: Varin
authors.forename: G B
authors.initials: GB
authors.lastname: Pajno
authors.forename: G
authors.initials: G
authors.lastname: Passalacqua
mesh_terms: D000293:Adolescent; D000485:Allergens / Q000032:analysis / Q000276:immunology*; D000641:Ammonia; D002648:Child; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000097:blood; D007840:Latex / Q000276:immunology*; D020315:Latex Hypersensitivity / Q000175:diagnosis*; D008297:Male; D011852:Radioallergosorbent Test; D012680:Sensitivity and Specificity; D012882:Skin Tests
chemical_list: D000485:Allergens; D007840:Latex; D007073:Immunoglobulin E; D000641:Ammonia