Alleviation of the cytotoxic activity induced by trans10, cis12-conjugated linoleic acid in rat hepatoma dRLh-84 cells by oleic or palmitoleic acid.
We showed the inhibitory effect of oleic and palmitoleic acids (OA and POA) on this trans10, cis12 (10t, 12c)-CLA induced cytotoxic activity. When cells were cultured in the presence of 10t, 12c-CLA, this potent cytotoxic effect on dRLh-84 cells was clearly obvious when compared to the control vehicle group. It was revealed that the levels of cellular OA and POA levels decreased upon cis9, trans11 (9c,11t) or 10t, 12c-CLA treatment in a time course dependent manner. OA or POA demonstrates a dose dependent inhibition of the 10t, 12c-CLA induced cytotoxicity. Notable nuclear fragmentation or activation of caspase-3 and 9 by 10t, 12c-CLA in dRLh-84 was counteracted by treatment with OA or POA. Results also suggest that 10t, 12c-CLA induced apoptosis can be inhibited by treatment with OA or POA.
Yamasaki
Masao
M
R&D division of Omu Milk Products Co. Ltd, 1-38-1 Shin katsudachi Omuta, Fukuoka 836-0895, Japan. masawo@agr-kyushu-u.ac.jp
Chujo
Hitomi
H
Nou
Shinsuke
S
Tachibana
Hirofumi
H
Yamada
Koji
K
D000818:Animals; D053148:Caspase 3; D053453:Caspase 9; D020169:Caspases / Q000378:metabolism; D005229:Fatty Acids, Monounsaturated / Q000494:pharmacology*; D019787:Linoleic Acid / Q000494:pharmacology*; D008113:Liver Neoplasms / Q000201:enzymology / Q000473:pathology*; D008114:Liver Neoplasms, Experimental / Q000201:enzymology / Q000473:pathology*; D019301:Oleic Acid / Q000494:pharmacology*; D051381:Rats; D014407:Tumor Cells, Cultured
D005229:Fatty Acids, Monounsaturated; C008757:palmitoleic acid; D019301:Oleic Acid; D019787:Linoleic Acid; C505410:Casp3 protein, rat; C506435:Casp9 protein, rat; D053148:Caspase 3; D053453:Caspase 9; D020169:Caspases