Programmed adsorption and release of proteins in a microfluidic device.
A microfluidic device has been developed that can adsorb proteins from solution, hold them with negligible denaturation, and release them on command. The active element in the device is a 4-nanometer-thick polymer film that can be thermally switched between an antifouling hydrophilic state and a protein-adsorbing state that is more hydrophobic. This active polymer has been integrated into a microfluidic hot plate that can be programmed to adsorb and desorb protein monolayers in less than 1 second. The rapid response characteristics of the device can be manipulated for proteomic functions, including preconcentration and separation of soluble proteins on an integrated fluidics chip.
Huber
Dale L
DL
Sandia National Laboratory, Post Office Box 5800, Albuquerque, NM 87185-1413, USA.
Manginell
Ronald P
RP
Samara
Michael A
MA
Kim
Byung-Il
BI
Bunker
Bruce C
BC
D000180:Acrylic Resins; D000327:Adsorption; D001671:Biochemistry / Q000295:instrumentation / Q000379:methods*; D003574:Cytochrome c Group / Q000737:chemistry; D006454:Hemoglobins / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D008832:Microchemistry; D008904:Miniaturization; D011506:Proteins / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
D000180:Acrylic Resins; D003574:Cytochrome c Group; D006454:Hemoglobins; D011506:Proteins; C052970:poly-N-isopropylacrylamide; D012710:Serum Albumin, Bovine