Evaluation of a commercially available ELISA kit as a tool to determine BTEX in groundwater.
The reliability of enzyme-linked immunosorbent assay (ELISA) tests as a screening technique to address groundwater contamination was tested in an area following leakage of gasoline from a petrol station. Immunoassay data of benzene, toluene, ethylbenzene, and o-, m- and p-xylene (BTEX) were compared with results obtained using capillary gas chromatographic analysis. Detection limits were of 20 microg l(-1) for ELISA and 0.3 microg l(-1) for gas chromatography with flame ionization and photoionization detectors (GC-FID/PID) determination. Despite an observed overestimation of BTEX concentrations as given by ELISA, the tests responded reliably to different levels of contamination.
Francioni
E
E
Pontifícia Universidade Católica do Rio de Janeiro, Depto. de Química, Marques de São Vicemte, 225, Gávea, Rio de Janeiro, RJ, Brazil, 22453-900.
Fillmann
G
G
Hamacher
C
C
Wagener
A De Luca R
Ade L
Depledge
M H
MH
Readman
J W
JW
Meniconi
M De Fátima Guadalupe
Mde F
D000906:Antibodies / Q000276:immunology; D001554:Benzene / Q000032:analysis*; D001555:Benzene Derivatives / Q000032:analysis* / Q000276:immunology; D004784:Environmental Monitoring / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D005742:Gasoline; D012680:Sensitivity and Specificity; D012989:Soil Pollutants / Q000032:analysis*; D014050:Toluene / Q000032:analysis* / Q000276:immunology; D014874:Water Pollutants, Chemical / Q000032:analysis*; D014992:Xylenes / Q000032:analysis* / Q000276:immunology
D000906:Antibodies; D001555:Benzene Derivatives; D005742:Gasoline; D012989:Soil Pollutants; D014874:Water Pollutants, Chemical; D014992:Xylenes; D014050:Toluene; D001554:Benzene; C004912:ethylbenzene