Analgesic effect of intrathecally administered matrix metalloproteinase 2 (MMP-2) in the rat formalin test.
Matrix metalloproteinases (MMPs) are a family of extracellular endopeptidases that selectively degrade components of the extracellular matrix. In the present study, the authors examined the effect of intrathecal injection of MMP-2 on the nociceptive transmission during the rat formalin test (a model of inflammatory pain). The paw formalin injection induces biphasic flinching (phase 1: 0-6 min; phase 2: 10-60 min) of the injected paw. Intrathecal injection of 1 microg of MMP-2 depressed the phase 1 agitation behavior, but not the phase 2 agitation behavior, and this effect of MMP-2 was antagonized by ONO-4817, an MMP inhibitor. ONO-4817 itself had no effect on the formalin test. These data suggest that exogenously applied MMP-2 to the spinal cord produces analgesic effects in the rat formalin test.
Yamamoto
Tatsuo
T
Department of Anesthesiology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba-shi, Chiba 260-8670, Japan. yamatat@med.m.chiba-u.ac.jp
Saito
Osamu
O
Shono
Koyo
K
Ohtori
Seiji
S
Ino
Hidetoshi
H
D000700:Analgesics / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D007278:Injections, Spinal; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000008:administration & dosage / Q000494:pharmacology* / Q000502:physiology; D010146:Pain / Q000503:physiopathology*; D010147:Pain Measurement; D010647:Phenyl Ethers / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
D000700:Analgesics; C414368:N-hydroxy-5-ethoxymethyloxy-2-methyl-4-(4-phenoxybenzoyl)aminopentanamide; D010647:Phenyl Ethers; D011480:Protease Inhibitors; D020778:Matrix Metalloproteinase 2