A PCR-based method for identification of bifidobacteria from the human alimentary tract at the species level.
A polymerase chain reaction (PCR)-based method was developed for the identification of isolates of Bifidobacterium at the species level. Using two Bifidobacterium-specific primers directed against the 16S ribosomal gene (Bif164 and Bif662), a PCR product was obtained from the type strains of 12 different bifidobacterial species that have been found in the human alimentary tract. After purification of the PCR products, the DNA was restricted with five different restriction enzymes. The size of the different restriction fragments was used as a fingerprint for the identification of individual bifidobacterial species. The amplified ribosomal DNA restriction analysis method was subsequently used to speciate bifidobacterial isolates from child's feces and from an in vitro model of the large intestine.
Venema
Koen
K
TNO Nutrition and Food Research, Department of Nutritional Physiology, P.O. Box 360, 3700 AJ Zeist, The Netherlands. venema@voeding.tno.nl
Maathuis
Annet J H
AJ
D001644:Bifidobacterium / Q000235:genetics* / Q000302:isolation & purification*; D002648:Child; D004269:DNA, Bacterial / Q000032:analysis; D005243:Feces / Q000382:microbiology; D006801:Humans; D007420:Intestine, Large / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D015183:Restriction Mapping
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S