title: EPMA observation between dentin and resin interfaces. Part 1. Comparison of wet and dry technique after short-term stored in water.
abstract: The study was to evaluate the marginal leakage of wet or dry dentin condition after restoration on short-term. In the study we used Photobond adhesive system and Single Bond adhesive system. The specimens were prepared from premolars. A class V cavity was prepared at the CEJ with a high-speed hand piece and #010 round diamond point. The cavity's walls and floors were etched with phosphoric acids of the test materials by wet and dry bonding techniques. The specimens were cross-sectioned longitudinally through the center of the cavities with a low speed diamond micro-cutter and polished with carbide paper (#600-1200) after storage in distilled water for 1 day, and silver nitrate staining. Specimens were viewed with EPMA for elemental distribution of calcium, nitrogen and silver on the resin-dentin interface. The uptake of silver particles was less in samples treated with the wet-bonding technique when compared with dry-bonding technique. This in vitro study showed that bonding technique is important in establishing a seal along the restoration margins to control marginal leakage.
authors.affiliation: Division of Cariology, Niigata University Graduate School of Medical and Dental Science for Oral Life Science, 5274 Gakkocho-dori 2-bancho Niigata 951-8514, Japan.
authors.forename: Linlin
authors.initials: L
authors.lastname: Han
authors.forename: Akira
authors.initials: A
authors.lastname: Okamoto
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Ishikawa
authors.forename: Masaaki
authors.initials: M
authors.lastname: Iwaku
mesh_terms: D000134:Acid Etching, Dental; D002118:Calcium / Q000032:analysis; D003188:Composite Resins / Q000737:chemistry; D001840:Dental Bonding / Q000379:methods*; D003737:Dental Cavity Preparation / Q000145:classification; D003763:Dental Leakage / Q000145:classification; D003804:Dentin / Q000648:ultrastructure*; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D003890:Desiccation; D004577:Electron Probe Microanalysis; D006801:Humans; D008855:Microscopy, Electron, Scanning; D009584:Nitrogen / Q000032:analysis; D012834:Silver / Q000032:analysis; D013499:Surface Properties; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D003188:Composite Resins; D017220:Dentin-Bonding Agents; D014867:Water; D012834:Silver; D009584:Nitrogen; D002118:Calcium