title: [Molecular characterization of hantavirus Shandong isolate JNL virus strain].
abstract: OBJECTIVE
  To understand the molecular epidemiologic characteristics of hantavirus Shandong isolate JNL virus strain.
  
  
  METHODS
  The complete M and S gene of the JNL virus isolated from Shandong Province was amplified by RT- PCR, and the purified PCR product was cloned into T vector for sequencing.
  
  
  RESULTS
  The results revealed that the JNL M segment was 3615 bp in length, encoding 1135 amino acids, and the S segment was 1698 bp encoding 429 amino acids, JNL belongs to HTN virus. The comparison of homology with HTN and SEO types showed that the difference of M and S complete sequences between JNL and all other HTN virus strains reached 20.0%-20.6%, and 15.5%-16.0%, respectively. Phylogenetic tree also showed that the position of JNL is located at a different clade.
  
  
  CONCLUSIONS
  HTN virus Shandong local isolate JNL strain is a new specific HTN subtype virus.
authors.affiliation: National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China.
authors.forename: Zhi-hui
authors.initials: ZH
authors.lastname: Yao
authors.forename: Yong-xin
authors.initials: YX
authors.lastname: Yu
authors.forename: Guan-mu
authors.initials: GM
authors.lastname: Dong
authors.forename: Xiang-zhi
authors.initials: XZ
authors.lastname: Meng
authors.forename: Zhao-hang
authors.initials: ZH
authors.lastname: Jin
mesh_terms: D004279:DNA, Viral / Q000032:analysis; D018063:Hantaan virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006480:Hemorrhagic Fever with Renal Syndrome / Q000821:virology*; D006801:Humans; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004279:DNA, Viral