title: Expression of local renin and angiotensinogen mRNA in cirrhotic portal hypertensive patient.
abstract: OBJECTIVE
  To investigate the expression of local renin and angiotensinogen mRNA in cirrhotic portal hypertensive patients.
  
  
  METHODS
  The expression of local renin and angiotensinogen mRNA in the liver, splenic artery and vein of PH patients was detected by RT-PCR analysis.
  
  
  RESULTS
  Expression of local renin mRNA in the liver of control group was (0.19+/-0.11), significantly lower than that in splenic artery (0.45+/-0.17) or splenic vein(0.39+/-0.12) respectively, (P<0.05). Expression of local angiotensinogen mRNA in the liver was (0.64+/-0.21), significantly higher than that in splenic artery(0.41+/-0.15) or in splenic vein (0.35+/-0.18) respectively, (P<0.05). Expression of local renin mRNA in the liver, splenic artery and vein of PH group was (0.78+/-0.28), (0.86+/-0.35) and (0.81+/-0.22) respectively, significantly higher than that in the control group, (P<0.05). Expression of local angiotensinogen mRNA in the liver, splenic artery and vein of PH group was (0.96+/-0.25), (0.83+/-0.18) and (0.79+/-0.23) respectively, significantly higher than that in the control group, (P<0.05). There was no significant difference between the liver, splenic artery and vein in the expression of local renin or local angiotensinogen mRNA in PH group, (P<0.05).
  
  
  CONCLUSIONS
  In normal subjects the expression of local renin and angiotensinogen mRNA was organ specific, but with increase of the expression of LRAS, the organ-specificity became lost in cirrhotic patients. LRAS may contribute to increased resistance of portal vein with liver and formation of splanchnic vasculopathy.
authors.affiliation: Department of General Surgery, Shandong Provincial Hospital, Jinan 250021, Shandong Province, China. pzzl@sina.com
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Yang
authors.forename: Bao-Min
authors.initials: BM
authors.lastname: Shi
authors.forename: Da-Peng
authors.initials: DP
authors.lastname: Li
authors.forename: Chong-Yun
authors.initials: CY
authors.lastname: Fang
authors.forename: Fa-Zu
authors.initials: FZ
authors.lastname: Qiu
mesh_terms: D000328:Adult; D000368:Aged; D000808:Angiotensinogen / Q000235:genetics*; D004730:Endothelium, Vascular / Q000473:pathology / Q000648:ultrastructure; D005260:Female; D015870:Gene Expression; D006801:Humans; D006975:Hypertension, Portal / Q000473:pathology / Q000503:physiopathology*; D007150:Immunohistochemistry; D008103:Liver Cirrhosis / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D009928:Organ Specificity; D012333:RNA, Messenger / Q000032:analysis; D012083:Renin / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013162:Splenic Vein / Q000473:pathology
chemical_list: D012333:RNA, Messenger; D000808:Angiotensinogen; D012083:Renin