Rotational-echo double resonance of uniformly labeled 13C clusters.
The use of rotational-echo double resonance NMR to measure distances from an observed tightly coupled cluster of 13C spins to a distant 15N, 31P, or 19F is practical if 13C chemical shifts and homonuclear 13C-13C isotropic J interactions are refocused by a combination of rotor-synchronized 13C pi and pi/2 pulses. This scheme is illustrated by experiments performed on diluted and recrystallized L-[13C(3),15N]alanine and L-[13C(6),alpha-15N]histidine.
Mehta
Anil K
AK
Department of Chemistry, Washington University, One Brookings Drive, Campus Box 1134, St. Louis, MO 63130-4899, USA.
Schaefer
Jacob
J
D000409:Alanine / Q000737:chemistry*; D002247:Carbon Isotopes; D006639:Histidine / Q000737:chemistry*; D008280:Magnetics; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D012399:Rotation
D002247:Carbon Isotopes; D006639:Histidine; D000409:Alanine