Comprehensive two-dimensional high-performance liquid chromatography for the separation of polycyclic aromatic hydrocarbons.
A comprehensive two-dimensional HPLC system for the separation of polycyclic aromatic hydrocarbons was developed using a pentabromobenzyl column as the first dimension and two short monolithic C18 columns as the second dimension. The primary column and two secondary columns were coupled by a 10-port 2-position valve. The effluent from the first dimension was repetitively injected into the second dimension every 12 s. Due to its resolution, this technique is a powerful tool for the separation of polycyclic aromatic hydrocarbons in a complex matrix such as environmental samples.
Murahashi
Tsuyoshi
T
Kyoto Pharmaceutical University, 5 Nakauchi-cho, Misasagi, Yamashina-ku, 607-8414 Kyoto, Japan. tmu@mb.kyoto-phu.ac.jp
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004784:Environmental Monitoring / Q000379:methods*; D005742:Gasoline / Q000032:analysis*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis*; D001335:Vehicle Emissions
D005742:Gasoline; D011084:Polycyclic Aromatic Hydrocarbons; D001335:Vehicle Emissions