title: Forsythia fructus inhibits the mast-cell-mediated allergic inflammatory reactions.
abstract: Mast cells are key as effector cells in the early phase allergic inflammation and in diverse immunological and pathological processes. Forsythia fructus (F. fructus) has used as a traditional medicine for inflammatory diseases. In the present study, we determined the effect of F. fructus extracts on compound 48/80-induced paw oedema and vascular permeability in vivo. In addition, we investigated in vitro whether F. fructus has inhibitory effects on compound 48/80-induced histamine releases from rat peritoneal mast cells (RPMC), and on phorbol 12-myristate 13-acetate (PMA) plus A23187-induced tumor necrosis factor-alpha (TNF-alpha) releases from human mast cells (HMC-1). In mice orally administrered F. fructus (100 microg/g) for 1 h, compound-48/80-induced oedema and vascular permeability were significantly reduced rather than those receiving intravenous injection of ketotifen, mast cell stabilizer. F. fructus dose-dependently inhibited the histamine release induced by compound 48/80 from RPMCs. Moreover, F. fructus had no cytotoxic effects on cell viability and had inhibitory effects on TNF-alpha secretion from HMC-1. These results suggest that F. fructus is a potential herb medicine for treatment of inflammatory diseases through downmodulating mast cell activation.
authors.affiliation: Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Iksan, Jeonbuk, 571-749, South Korea.
authors.forename: Mi-Sun
authors.initials: MS
authors.lastname: Kim
authors.forename: Ho-Jeong
authors.initials: HJ
authors.lastname: Na
authors.forename: Seung-Woo
authors.initials: SW
authors.lastname: Han
authors.forename: Jong-Sik
authors.initials: JS
authors.lastname: Jin
authors.forename: Un-Yong
authors.initials: UY
authors.lastname: Song
authors.forename: Eon-Jeong
authors.initials: EJ
authors.lastname: Lee
authors.forename: Bong-Keun
authors.initials: BK
authors.lastname: Song
authors.forename: Seung-Heon
authors.initials: SH
authors.lastname: Hong
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Kim
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology* / Q000627:therapeutic use; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004487:Edema / Q000378:metabolism / Q000517:prevention & control*; D031657:Forsythia / Q000502:physiology*; D006801:Humans; D006967:Hypersensitivity / Q000378:metabolism / Q000517:prevention & control*; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats
chemical_list: D018926:Anti-Allergic Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D010936:Plant Extracts