High transfection efficiency of poly(4-vinylimidazole) as a new gene carrier.
Poly(4-vinylimidazole) (P4V) was obtained by free radical polymerization of 4-vinylimidazole (4V) prepared by decarboxylation of urocanic acid. P4V formed a complex with DNA that exhibited higher transfection effiency on Hela cells than polyethylenimine (PEI), through the proton sponge mechanism of the imidazole groups in the side chain of the P4V, and low cell toxicity.
Ihm
Jong-Eun
JE
School of Agricultural Biotechnology, Seoul National University, Suwon 441-744, Korea.
Han
Ki-Ok
KO
Han
In-Kwon
IK
Ahn
Kwang-Duk
KD
Han
Dong-Keun
DK
Cho
Chong-Su
CS
D016475:3T3 Cells; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D015316:Genetic Therapy / Q000379:methods; D006367:HeLa Cells; D006801:Humans; D007093:Imidazoles / Q000138:chemical synthesis* / Q000737:chemistry* / Q000494:pharmacology; D051379:Mice; D008956:Models, Chemical; D008970:Molecular Weight; D010316:Particle Size; D011145:Polyvinyls / Q000138:chemical synthesis* / Q000737:chemistry* / Q000494:pharmacology; D014162:Transfection / Q000379:methods*
D004337:Drug Carriers; D007093:Imidazoles; D011145:Polyvinyls; C482913:poly(4-vinylimidazole); D004247:DNA