A new method to correlate histology with biomechanical properties in urethral tissue. An in-vitro study using light microscopy and scanning acoustic microscopy.
When surveying the classical biomechanical theory of flow and resistance, the passive elastic properties of the urethra seems to be important for the transport of urine though the urethra. The aim of this study was to show that scanning acoustic microscopy (SAM) is a suitable methodology for investigating elastic properties of the urethra, and that it can be used to correlate elastic properties to histological areas. One 40 kg female pig and one 2 kg male rabbit comprised the material. A SAM2000 was used at a working frequency of 1000 MHz. Sections of nominal 3 micrometer thickness fixed urethral tissue were prepared for SAM and stained for light microscopy. The histological layers of the urethra were evident in the SAM image, and showed highly variable values of elastic properties. The layers seen with SAM correlated well with those seen with light microscopy. In conclusion, we have provided the first images of the microelastic properties of the urethra and correlated them to histology.
Andersen
Thomas
T
Institute of Experimental Clinical Research, University of Aarhus, Aarhus University Hospital, Aarhus, Denmark. ta@iekf.au.dk
Jensen
Anne Skakkebaek
AS
Lalla
Marianna
M
Jørgensen
Claus Schiøtt
CS
Jørgensen
Troels Munch
TM
D000818:Animals; D004548:Elasticity; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D033401:Microscopy, Acoustic; D011817:Rabbits; D013552:Swine; D014521:Urethra / Q000166:cytology*