[Expression of survivin in cord blood CD34(+) stem/progenitor cells and its significance].
OBJECTIVE
To investigate the expression and significance of survivin protein and mRNA in cord blood (CB) CD(34)(+) stem/progenitor cells.


METHODS
Survivin level in CB CD(34)(+) cells was assessed by immuno-histochemistry and reverse transcription polymerase chain reaction (RT-PCR).


RESULTS
Survivin expressed in fresh CB CD(34)(+) cells, its protein level was 9.4 +/- 1.2, faint signal of the mRNA transcript band was detected. In the in vitro culture of the cells, hematopoietic growth factors could upregulate survivin expression, especially the combination of SCF, FL and Tpo. After 3 days culture, its protein level was 25.2 +/- 5.3, and mRNA transcript increased significantly. Survivin expression dropped down 24 hours after withdraw of these cytokines.


CONCLUSIONS
Survivin is not a specific anti-apoptotic protein of cancer, and also expressed in normal hematopoietic stem/progenitor cells. It plays important regulating roles in the process of hematopoiesis.
Ma
Yan-ping
YP
Department of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Zou
Ping
P
Xiao
Juan
J
Huang
Shi-ang
SA
D018952:Antigens, CD34 / Q000097:blood; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology*; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000077022:Survivin
D018952:Antigens, CD34; C107489:BIRC5 protein, human; D016298:Hematopoietic Cell Growth Factors; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D000077022:Survivin