Continuous penicillin G hydrolysis in an electro-membrane reactor with immobilized penicillin G acylase.
Penicillin G (2%, w/v in phosphate buffer, pH 8) was hydrolysed in a flow-through, miniature electro-membrane reactor with the penicillin G acylase immobilized in 5% (w/v) polyacrylamide (diam. 10 mm, thickness 2.6 mm, enzyme activity 24 U ml(-1)). The conversion of penicillin G increased from 0.15 to almost 0.5 when the electric current applied to the reactor was changed from -600 to +600 A/m2 with a substrate residency of 1 h.
Zadrazil
Ales
A
Institute of Chemical Technology, Prague, Department of Chemical Engineering & Center for Nonlinear Dynamics of Chemical and Biological Systems, Technická 5, 166 28 Praha 6, Czech Republic.
Chmelíková
Růzena
R
Hasal
Pavel
P
D019149:Bioreactors; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004574:Electromagnetic Fields; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D006868:Hydrolysis; D008567:Membranes, Artificial; D010397:Penicillanic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D010399:Penicillin Amidase / Q000737:chemistry*; D010400:Penicillin G / Q000737:chemistry*
D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D010397:Penicillanic Acid; D010399:Penicillin Amidase; D010400:Penicillin G; C004408:aminopenicillanic acid