Association of herpesviruses in the aqueous humor of patients with serpiginous choroiditis: a polymerase chain reaction-based study.
OBJECTIVE
To determine the presence of herpesvirus DNA in the aqueous humor (AH) of patients with serpiginous choroiditis using polymerase chain reaction (PCR).


METHODS
AH from nine patients previously diagnosed with serpiginous choroiditis were investigated for herpes simplex virus (HSV), varicella zoster virus (VZV), and cytomegalovirus (CMV) by conventional virological methods and PCR. The PCR-positive DNA was gel-purified, extracted, and sequenced using a dye-based Applied Biosystems procedure. The sequences were processed through the National Cancer Institute's BLAST inquiry for species identification.


RESULTS
Culture and cytological examination of AH from all nine patients were negative for HSV, VZV, and CMV. Five were positive for VZV, one was positive for HSV, and three were wholly negative using PCR. Subsequent DNA sequencing of the positive samples authenticated the presence of VZV and HSV DNA in the respective patients.


CONCLUSIONS
VZV and HSV DNA were detected in a subset of patients with serpiginous choroiditis, suggesting that these viruses may function in the pathogenesis of this disease.
Priya
Kannian
K
Vision Research Foundation, Sankara Nethralaya, Chennai, India.
Madhavan
Hajib N
HN
Reiser
Bibiana J
BJ
Biswas
Jyotrimay
J
Saptagirish
Rambhatla
R
Narayana
Kannan M
KM
Rao
Narsing A
NA
D000328:Adult; D001082:Aqueous Humor / Q000821:virology*; D002833:Choroiditis / Q000473:pathology / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005654:Fundus Oculi; D006564:Herpesviridae / Q000302:isolation & purification*; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000821:virology*; D014645:Herpesvirus 3, Human / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D018139:Simplexvirus / Q000235:genetics / Q000302:isolation & purification
D004279:DNA, Viral