Alpha-defensin 1 (human neutrophil protein 1) as an antichemotactic agent for human polymorphonuclear leukocytes.
Medium conditioned by tumor necrosis factor alpha (TNF-alpha)-stimulated polymorphonuclear leukocytes (PMN) (CM-TNF) suppresses PMN migration. Therefore, we wished to identify the agent(s) in CM-TNF that mediated antichemotactic activity. CM-TNF was fractionated by high-performance liquid chromatography, and one fraction with antichemotactic activity contained the bactericidal protein human neutrophil protein 1 (HNP-1). We showed that HNP-1 suppresses PMN migration to formyl-methionyl-leucyl-phenylalanine but not to interleukin 8.
Grutkoski
P S
PS
Department of Surgery, Rhode Island Hospital, Providence, Rhode Island 02903, USA.
Graeber
C T
CT
Lim
Y P
YP
Ayala
A
A
Simms
H H
HH
D015153:Blotting, Western; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D066298:In Vitro Techniques; D016209:Interleukin-8 / Q000494:pharmacology; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects*; D023062:Receptors, Interleukin-8A / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D023084:alpha-Defensins / Q000494:pharmacology*
D016209:Interleukin-8; D023062:Receptors, Interleukin-8A; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D023084:alpha-Defensins; D009240:N-Formylmethionine Leucyl-Phenylalanine