Inhibition on telomerase activity and cytotoxic effects by cisplatin in cultured human choroidal melanoma cells.
OBJECTIVE
To study the changes of telomerase activity and cytotoxic effects by Cisplatin; cis-dichlorodiamine platinum (CDDP) in cultured human choroidal melanoma.


METHODS
The primary cultured human choroidal melanoma cells were cultured in the presence and absence of CDDP with different concentration and time respectively. The toxic effects were evaluated by MTT and the level of telomerase was detected by PCR-ELISA assay. And the relationship between telomerase activity and cytotoxic effects were analyzed by a correlation analysis.


RESULTS
Following the increase of the concentration and the time of CDDP, gradually repressed telomerase activity was detected in cultured cells. Meanwhile, the restrain rate of the cells increased. The telomerase activity at 24 h and 1 microgram/ml was repressed significantly compared with the control cells. However, the appearance of cell death lagged behind the decreasing of telomerase.


CONCLUSIONS
CDDP is an effective telomerase inhibitor in cultured choroidal melanoma cells of human eyes, which presents concentration and time dependency and can cause the death of cultured cells.
Cheng
Hao
H
Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.
Wu
Zhongyao
Z
Zheng
Jianliang
J
Lu
Guilan
G
Yan
Jianhua
J
Liu
Min
M
Huang
Danping
D
Lin
Jianxian
J
D000328:Adult; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D016923:Cell Death / Q000187:drug effects; D002830:Choroid Neoplasms / Q000201:enzymology / Q000473:pathology*; D002945:Cisplatin / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008297:Male; D008545:Melanoma / Q000201:enzymology / Q000473:pathology*; D008875:Middle Aged; D019098:Telomerase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D019098:Telomerase; D002945:Cisplatin