Screening and characterization of astaxanthin-hyperproducing mutants of Haematococcus pluvialis.
Haematococcus pluvialis was mutated by UV or ethyl methanesulphonate. Mutants resistant to nicotine, diphenylamine, fluridone or norflurazon were then selected. Several nicotine-resistant mutants showed increased (1.9% to 2.5% vs. 1.2% w/w) astaxanthin production. Mutants maintained high astaxanthin production over 4 months of repeated culture.
Chen
Yong
Y
National Feed Engineering Technology Research Center, China Agricultural University, Beijing 100094, P.R. China.
Li
Defa
D
Lu
Wenqing
W
Xing
Jianjun
J
Hui
Bodi
B
Han
Yashan
Y
D001709:Biotechnology / Q000379:methods*; D000460:Chlorophyta / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D004159:Diphenylamine / Q000494:pharmacology; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D016296:Mutagenesis; D009154:Mutation; D009538:Nicotine / Q000494:pharmacology; D011724:Pyridazines / Q000494:pharmacology; D011728:Pyridones / Q000494:pharmacology; D014466:Ultraviolet Rays; D024341:Xanthophylls; D019207:beta Carotene / Q000031:analogs & derivatives* / Q000096:biosynthesis*
D011724:Pyridazines; D011728:Pyridones; D024341:Xanthophylls; D019207:beta Carotene; C013351:fluridone; D009538:Nicotine; C005948:astaxanthine; D005020:Ethyl Methanesulfonate; D004159:Diphenylamine; C008677:norflurazone