The influences of extremely low frequency magnetic fields on drug-induced convulsion in mouse.
This study investigated the effects of extremely low frequency magnetic fields (ELF-MFs) on the sensitivity of seizure response to bicuculline, picrotoxin and NMDA in mice. The mice were exposed to either a sham or 20 G ELF-MFs for 24 hours. Convulsants were then administered i.p. at various doses. The seizure induction time and duration were measured and lethal dose (LD50) and convulsant dose (CD50) of the clonic and tonic convulsion were calculated. The analysis of glutamate, glycine, taurine and GABA of mouse brain was accomplished by HPLC. The mice exposed to ELF-MFs showed moderately higher CD50, LD50 and onset time on the bicuculline-induced seizure. However, the ELF-MFs did not influence them in the NMDA and picrotoxin-induced seizures. After the exposure to MFs exposure, the glutamate level was increased and GABA was decreased significantly in NMDA and picrotoxin-induced seizure. The level of glutamate and GABA were not changed by MFs in bicuculline-induced seizure. These results suggest that ELF-MFs may alter the convulsion susceptibility through GABAergic mechanism with the involvement of the level of glutamate and GABA.
Sung
Ji Hyun
JH
Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.
Jeong
Ji Hoon
JH
Kim
Jeong Soo
JS
Choi
Tai Sik
TS
Park
Joon Hong
JH
Kang
Hoe Yun
HY
Kim
Young Sil
YS
Kim
Dong Suk
DS
Sohn
Uy Dong
UD
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D003292:Convulsants / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004574:Electromagnetic Fields / Q000009:adverse effects; D051379:Mice; D008813:Mice, Inbred ICR; D012640:Seizures / Q000139:chemically induced* / Q000378:metabolism
D003292:Convulsants