Observation and analysis of single DNA nano-kinetics by pin-fiber video scope.
The nano-kinetic movement of a single DNA molecule was observed and analyzed by a newly developed video-microscope system with an optical fiber, called a pin-fiber video scope. A single lambda-DNA molecule was put in focus using fiber-illumination, and the stretching and shrinking motion was measured. The molecule's kinetics were analyzed by numerical calculations and are discussed. A photocleavage phenomenon of DNA molecules was also visualized by the pin-fiber video scope. The new video-microscope system has the potential to observe and analyze the nano-kinetics of a single molecule.
Hirakawa
Yasuyuki
Y
Graduate School of Medical Science, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan. yhirakaw@hiroshima-u.ac.jp
Suzuto
Masashi
M
Ohnishi
Hitoshi
H
Shingaki
Tomoteru
T
Eyring
Edward M
EM
Tokunaga
Makio
M
Masujima
Tsutomu
T
D004247:DNA / Q000378:metabolism*; D005336:Fiber Optic Technology; D006801:Humans; D018715:Microscopy, Video; D036103:Nanotechnology / Q000379:methods*; D055100:Optical Fibers
D004247:DNA