Redistribution of motor proteins by Cytochalasin J treatment.
In this study we extend our analysis of the effect of Cytochalasin J (CJ) on mitotic and interphase cells by the use of immunocytochemical techniques to localize antigens to anti-beta-tubulin, anti-dynein heavy chain (HC), anti-dynein intermediate chain (IC), and anti-kinesin antibodies following CJ treatment. Anti-dynein IC and HC staining of CJ treated cells showed a significant reduction in anti-dynein staining in the nuclear region of interphase cells. Monolayer cultures of PtK(1)cells treated with 10 microg/ml CJ for 10 min showed a significant reduction in pixel luminosity of fluorescence staining using anti-dynein IC and HC antibodies (P<0.05). Cytochalasin J treatment reorganized anti-dynein staining from a cytoplasmic punctate staining with greatest intensity in the perinuclear region, to a more uniform staining throughout the cytoplasm.
Robinson
Rollin W
RW
Department of Biological Sciences, University of Denver, 2190 E. Iliff Avenue, 80208, Denver, CO, USA.
Snyder
Judith A
JA
D000818:Animals; D002460:Cell Line / Q000737:chemistry / Q000187:drug effects; D003572:Cytochalasins / Q000494:pharmacology*; D003593:Cytoplasm / Q000378:metabolism; D004398:Dyneins / Q000187:drug effects / Q000378:metabolism; D007150:Immunohistochemistry; D007399:Interphase / Q000502:physiology; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism; D020409:Molecular Motor Proteins / Q000187:drug effects* / Q000378:metabolism; D008941:Spindle Apparatus / Q000187:drug effects / Q000378:metabolism
D003572:Cytochalasins; D020409:Molecular Motor Proteins; C106137:cytochalasin J; D004398:Dyneins