[Antigenic analysis of the common recombinant mutant types of HBsAg].
BACKGROUND
To study the difference between the mutant types and the wild type of HBsAg on the binding efficiency with antibodies in order to find some methods to detect the mutants.


METHODS
The recombinant wild type of HBsAg and the mutants including 145 R, 133 T, 126 S, 141 E, 126 S+133 T, 126 S+133 T+145 R and 126 S+133 T+141 E were cloned, respectively. Then the expression vector containing the wild or mutant gene was transfected into COS7 cells, and the recombinant proteins were expressed. The proteins were detected with the routine HBsAg kits.


RESULTS
The binding efficiency with monoclonal antibodies of HBsAg expressed by 126 S was higher than that of the wild type, while the results of 145 R, 141 E, 126 S+133 T+145 R and 126 S+133 T+141 E were lower than that of the wild, and 133 T was similar to the wild. But most of the mutants had the same reactivity with the polyclonal antibody.


CONCLUSIONS
The effect of mutations on antibody binding differs depending on the amino acid involved and on the location within the "a" loop. So it is necessary to use polyclonal antibody or to find new kits to detect the mutants of HBsAg.
Bai
Yu
Y
Institute for Viral Disease Control Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Xia
Guo-ling
GL
Jia
Zhi-yuan
ZY
Cong
Yu
Y
Wang
Xiao-li
XL
Guo
Min-zhuo
MZ
Zhan
Mei-yun
MY
D001666:Binding Sites, Antibody; D000939:Epitopes; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006514:Hepatitis B Surface Antigens / Q000235:genetics / Q000276:immunology; D006515:Hepatitis B virus / Q000235:genetics / Q000276:immunology; D006801:Humans; D009154:Mutation; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology
D000939:Epitopes; D006514:Hepatitis B Surface Antigens; D011994:Recombinant Proteins