title: Simple and rapid methods for SEM observation and TEM immunolabeling of rubber particles.
abstract: We developed a method involving air-drying of a rubber suspension after fixation in glutaraldehyde-tannic acid and postfixation in osmium tetroxide for SEM observation. For TEM immunolabeling the suspension was air-dried after osmium-only fixation. Whereas conventional methods failed to satisfactorily stabilize rubber particles, the methods described here proved successful in preserving their integrity.
authors.affiliation: Agricultural Plant Stress Research Center, Chonnam National University, Gwangju, Republic of Korea. adyasingh@hotmail.com
authors.forename: Adya P
authors.initials: AP
authors.lastname: Singh
authors.forename: Seung Gon
authors.initials: SG
authors.lastname: Wi
authors.forename: Hunseung
authors.initials: H
authors.lastname: Kang
authors.forename: Gap Chae
authors.initials: GC
authors.lastname: Chung
authors.forename: Yoon Soo
authors.initials: YS
authors.lastname: Kim
mesh_terms: D030681:Ficus / Q000737:chemistry; D005404:Fixatives; D028482:Hevea / Q000737:chemistry; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D012408:Rubber / Q000737:chemistry*
chemical_list: D005404:Fixatives; D012408:Rubber