[Studies on extraction and isolation of active constituents from Psoralen corylifolia L. and the antitumor effect of the constituents in vitro].
OBJECTIVE
To study the chemical constituents from Psoralea corylifolia L. and their antitumor activities.


METHODS
The constituents were isolated on silica gel column chromatography and identified by physicochemical properties and spectroscopic analysis. Morphological and MTT assays in vitro were used to explore the inhibiting effect of the active compounds on the proliferation of BGC-823 cancer cell.


RESULTS
Two compounds were isolated from its chloroform extract and identified as psoralen (I) and isopsoralen (II). IC50 of psoralen and isoporalen were 5.82 micrograms/ml and 148.8 micrograms/ml respectively.


CONCLUSIONS
Compound I and II having antitumor activity against BGC-823 cancer cell were obtained from this plant with the yields of 0.048% and 0.11%.
Guo
Jiangning
J
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016.
Wu
Hou
H
Weng
Xinchu
X
Yan
Jianhua
J
Bi
Kaishun
K
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology; D005363:Ficusin / Q000302:isolation & purification / Q000494:pharmacology*; D011564:Furocoumarins / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D020128:Inhibitory Concentration 50; D010946:Plants, Medicinal / Q000737:chemistry; D029907:Psoralea / Q000737:chemistry*; D013274:Stomach Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D011564:Furocoumarins; C011659:angelicin; D005363:Ficusin