Chip ligating human genomic DNA serves as storage material and template for polymerase chain reaction.
A chip was developed to store DNA for medical research. The optional restriction site fixed on the chip can randomly ligate with whole human genomic DNA treated by the corresponding restriction enzyme. PCR can then use the chip as template DNA. Moreover, a chip fixing two restriction sites (e.g. EcoRI and HindIII) showed the amplification by PCR for any location of genomic DNA. Repetitive PCRs have confirmed that a DNA chip can be stored by at -4 degrees C for 2 years.
Hanaoka
T
T
Epidemiology and Biostatistics Division, National Cancer Center Research Institute East, Chiba, Japan. thanaoka@east.ncc.go.jp
Takai
O
O
Takahashi
K
K
Tsugane
S
S
D004247:DNA / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification*; D004355:Drug Stability; D004356:Drug Storage / Q000379:methods*; D004867:Equipment Design; D006801:Humans; D016247:Information Storage and Retrieval / Q000379:methods; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods; D011309:Preservation, Biological / Q000295:instrumentation / Q000379:methods; D013048:Specimen Handling / Q000295:instrumentation* / Q000379:methods
D004247:DNA