Cell death unlikely contributes to taxol production in fungal elicitor-induced cell suspension cultures of Taxus chinensis.
Cell suspension cultures of Taxus chinensis, with 20, 40 and 100 mg fungal elicitor l(-1) from Aspergillus niger, underwent rapid cell death after 24 h, which was about 2, 3.7 and 5-fold of that of the control. At the same time, Taxol production was increased, respectively, to about 5, 8 and 3-fold of that of the control. Inhibition of phenolics biosynthesis resulted in a 150% increase in cell death but a 54% decrease in Taxol production compared with 40 mg elicitor l(-1) alone. O2-free N2 inhibited cell death but had little effect on Taxol production as induced by 40 mg fungal elicitor l(-1).
Lan
W Z
WZ
School of Life Science and Technology, Huazhong University, of Science and Technology, Wuhan 430074, China. lanwz73@sohu.com
Yu
L J
LJ
Li
M Y
MY
Qin
W M
WM
D001234:Aspergillus niger / Q000737:chemistry* / Q000302:isolation & purification; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D003470:Culture Media / Q000494:pharmacology; D005656:Fungal Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D010100:Oxygen / Q000378:metabolism; D017239:Paclitaxel / Q000096:biosynthesis*; D020946:Taxus / Q000187:drug effects* / Q000378:metabolism* / Q000502:physiology
D003470:Culture Media; D005656:Fungal Proteins; D017239:Paclitaxel; D010100:Oxygen