Comparison of the gene expression profiles of monocytic versus granulocytic lineages of HL-60 leukemia cell differentiation by DNA microarray analysis.
It is now recognized that precise patterns of differentially expressed genes ultimately direct a particular cell toward a given lineage. In this study, we compared the expression profiles of cancer-related genes by cDNA microarray analysis during the differentiation of human promyelocytic leukemia HL-60 cells into either monocytes or granulocytes. RNA was isolated at times 0, 6, 12, 24, 36, 48, and 72 h following stimulation of differentiation with all-trans retinoic acid (all-trans RA) or 1,25-dihydroxyvitamin D(3) [1,25-(OH)(2)D(3)], and hybridized to the microarray gene chips containing 872 genes related to cell-cycles, oncogenes and leukemias. Several genes were commonly or differentially regulated during cell differentiation into either lineage, as demonstrated by both hierarchical and self-organizing map clustering analysis. At 72 h the expression levels of 45 genes were commonly up- or down-regulated at least a twofold in both lineages. Most importantly, 32 genes including alpha-L-fucosidase gene and adducin gamma subunit gene were up- or down-regulated only in all-trans RA-treated HL-60 cells, while 12 genes including interleukin 1beta and hypoxia-inducible factor 1alpha were up- or down-regulated only in 1,25-(OH)(2)D(3)-treated HL-60 cells. The expression of selected genes was confirmed by Northern blot analysis. As expected, some genes identified have not been examined during HL-60 cell differentiation into either lineage. The identification of genes associated with a specific differentiation lineage may give important insights into functional and phenotypic differences between two lineages of HL-60 cell differentiation.
Song
Ju Han
JH
College of Pharmacy and Research Institute of Drug Development, Chonnam National University, Kwangju, South Korea.
Kim
Jong Man
JM
Kim
Seung Hyun
SH
Kim
Hyeoung Joon
HJ
Lee
Jae Jung
JJ
Sung
Myung Hui
MH
Hwang
Seung Yong
SY
Kim
Tae Sung
TS
D002117:Calcitriol / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D019070:Cell Lineage / Q000235:genetics; D002470:Cell Survival / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis; D020869:Gene Expression Profiling; D015973:Gene Expression Regulation, Leukemic; D006098:Granulocytes / Q000473:pathology / Q000502:physiology*; D018922:HL-60 Cells / Q000502:physiology; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics* / Q000473:pathology; D009000:Monocytes / Q000473:pathology / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D012334:RNA, Neoplasm / Q000032:analysis; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D012334:RNA, Neoplasm; D014212:Tretinoin; D002117:Calcitriol