title: Lysosomal storage disease in Sida carpinifolia toxicosis: an induced mannosidosis in horses.
abstract: BACKGROUND
  This study reports a neurological disease unrecognised until now in ponies in southern Brazil.
  
  
  OBJECTIVE
  Epidemiological data strongly suggests that the ingestion of Sida carpinifolia is involved in the aetiology. We tested the hypothesis that it is an acquired lyosomal storage disease.
  
  
  METHODS
  Following the death of 3 ponies, all ponies from the premises were closely monitored; epidemiological data and clinical findings carefully recorded. Fragments of several organs, including CNS, were fixed in neutral formalin and embedded in paraffin-wax. Sections were stained with haematoxylin and eosin. Representative sections of the cerebellum and trigeminal ganglia were submitted to lectin histochemical procedures.
  
  
  RESULTS
  The neurological disorder, characterised by stiff gait, muscle tremors, abdominal pain and death, was observed on a farm with 3 hectares of pasture. Three of 11 ponies died 15-20 days after they had been introduced into a new paddock heavily infested by the plant Sida carpinifolia. No significant gross lesions were observed. The main histological findings included multiple cytoplasmatic vacuoles in swollen neurones in the brain, cerebellum, spinal cord, autonomic ganglia (trigeminal and celiac ganglia), and submucosal and myenteric plexus of the intestines. In the kidneys, there was marked vacuolation of the proximal convoluted tubular cells. Sections of cerebellum and trigeminal ganglion were submitted to lectin histochemistry. The vacuoles in different cerebellar and ganglion cells reacted strongly to the following lectins: Concanavalia ensiformis, Triticum vulgaris and succinylated-Triticum vulgaris.
  
  
  CONCLUSIONS
  The pattern of staining coincides with that of both swainsonine toxicosis and inherited mannosidosis reports. The histopathological changes were similar to those described in S. carpinifolia spontaneous and experimental poisoning in goats. This disease seems to be similar to Swainsona, Oxytropis and Astragalus toxicosis.
  
  
  CONCLUSIONS
  S. carpinifolia should be evaluated as a possible cause in the diagnosis of equine neuropathies.
authors.affiliation: Department of Pathology, Veterinary Faculty, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
authors.forename: A P
authors.initials: AP
authors.lastname: Loretti
authors.forename: E M
authors.initials: EM
authors.lastname: Colodel
authors.forename: E J
authors.initials: EJ
authors.lastname: Gimeno
authors.forename: D
authors.initials: D
authors.lastname: Driemeier
mesh_terms: D000818:Animals; D001938:Brazil; D002490:Central Nervous System / Q000473:pathology; D002531:Cerebellum / Q000473:pathology; D006651:Histocytochemistry / Q000662:veterinary; D006734:Horse Diseases / Q000209:etiology* / Q000473:pathology; D006736:Horses; D037102:Lectins / Q000737:chemistry; D019660:Malvaceae / Q000506:poisoning*; D044904:Mannosidase Deficiency Diseases / Q000209:etiology / Q000473:pathology / Q000662:veterinary*; D010939:Plant Poisoning / Q000150:complications / Q000473:pathology / Q000662:veterinary*; D012668:Trigeminal Ganglion / Q000473:pathology
chemical_list: D037102:Lectins