title: Possible mechanism for the neuroprotective effects of L-carnitine on methamphetamine-evoked neurotoxicity.
abstract: Some of the damage to the CNS that is observed following amphetamine and methamphetamine (METH) administration is known to be linked to increased formation of free radicals. This increase could be, in part, related to mitochondrial dysfunction and/or cause damage to the mitochondria, thereby leading to a failure of cellular energy metabolism and an increase in secondary excitotoxicity. The actual neuronal damage that occurs with METH-induced toxicity seems to affect dopaminergic cells in particular. METH-induced toxicity is related to an increase in the generation of both reactive oxygen (hydroxyl, superoxide, peroxide) and nitrogen (nitric oxide) species. Peroxynitrite (ONOO(-)), which is a reaction product of either superoxide or nitric oxide, is the most damaging radical. It can be reduced by antioxidants such as selenium, melatonin, and the selective nNOS inhibitor, 7-nitroindazole. METH-induced toxicity has been previously shown to increase production of the peroxynitrite stress marker, 3-nitrotyrosine (3-NT), in vitro, in cultured PC12 cells, and also in vivo, in the striatum of adult male mice. Pre- and post-treatment of mice with l-carnitine (LC) significantly attenuated the production of 3-NT in the striatum after METH exposure. LC is a mitochondriotropic compound in that it carries long-chain fatty acyl groups into mitochondria for beta-oxidation. It was shown also to play a protective role against various mitochondrial toxins, such as 3-nitropropionic acid. The protective effects of LC against METH-induced toxicity could be related to its prevention of possible metabolic compromise produced by METH and the resulting energy deficits. In particular, LC may be maintaining the mitochondrial permeability transition (MPT) and modulating the activation of the mitochondrial permeability transition pores (mPTP), especially the cyclosporin-dependent mPTP. The possible neuroprotective mechanism of LC against METH-toxicity and the role of the mitochondrial respiratory chain and the generation of free radicals and their subsequent action on the MPT and mPTP are also being examined using an in vitro model of NGF-differentiated pheochromocytoma cells (PC12). In preliminary experiments, the pretreatment of PC12 cells with LC (5 mM), added 10 min before METH (500 micro M), indicated that LC enhances METH-induced DA depletion. The role of LC in attenuating METH-evoked toxicity is still under investigation and promises to reveal information regarding the underlying mechanisms and role of mitochondria in the triggering of cell death.
authors.affiliation: Research and Development, Sigma tau-HealthScience, Pomezia, Italy. ashraf.virmani@st-hs.it
authors.forename: Ashraf
authors.initials: A
authors.lastname: Virmani
authors.forename: Franco
authors.initials: F
authors.lastname: Gaetani
authors.forename: Syed
authors.initials: S
authors.lastname: Imam
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Binienda
authors.forename: Syed
authors.initials: S
authors.lastname: Ali
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002331:Carnitine / Q000494:pharmacology*; D002460:Cell Line; D000697:Central Nervous System Stimulants / Q000633:toxicity*; D004298:Dopamine / Q000378:metabolism; D005609:Free Radicals / Q000378:metabolism; D008694:Methamphetamine / Q000633:toxicity*; D008928:Mitochondria / Q000378:metabolism; D015394:Molecular Structure; D018696:Neuroprotective Agents / Q000494:pharmacology*
chemical_list: D000697:Central Nervous System Stimulants; D005609:Free Radicals; D018696:Neuroprotective Agents; D008694:Methamphetamine; D002331:Carnitine; D004298:Dopamine