title: Oxidative stress during leukocyte absorption apheresis.
abstract: Leukocyte absorption apheresis absorbs leukocytes to the apheresis columns involving leukocyte activation. This process is regarded as bioincompatible and avoided in hemodialysis or other extracorporeal circulation processes. Thus, leukocyte apheresis has a potential risk to exacerbate in vivo oxidative stress. We evaluated the changes in plasma oxidative stress during leukocyte apheresis. Patients diagnosed as ulcerative colitis (UC) and treated with leukocyte apheresis were studied. Adacolumn (celluloseacetate beads) or Cellsorba EX (polyethylenephtarate fiber) was used for the leukocyte absorption device. Oxidative stress was measured by thiobarbituric acid reactive substances (TBARS) and hydroxyl radical ((*)OH) scavenging activity. Plasma samples were collected from the pre- and post-column sampling port at the start, and from the pre-column sampling port at the end of the treatment. The (*)OH signal intensities (OHRI) significantly increased during a column passage, indicating a loss of plasma (*)OH scavenging activity. However, OHRI was reduced at the end, suggesting a recovery of radical scavenging activity during leukocyte apheresis. Significant decreases of OHRI and TBARS were only observed in the early phase of the therapeutic course. No differences of OHRI and TBARS levels were observed between the two columns. These results indicate that though the plasma antioxidant activity was diminished by a column passage, plasma antioxidant activity recovers during the procedure. This efficient antioxidative effect is limited to the early phase of the therapeutic course.
authors.affiliation: Institute of Clinical Medicine, University of Tsukuba, Tsukuba City, Japan. aki-hira@md.tsukuba.ac.jp
authors.forename: Aki
authors.initials: A
authors.lastname: Hirayama
authors.forename: Sohji
authors.initials: S
authors.lastname: Nagase
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ueda
authors.forename: Takashi
authors.initials: T
authors.lastname: Ishizu
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Taru
authors.forename: Keigyou
authors.initials: K
authors.lastname: Yoh
authors.forename: Kouichi
authors.initials: K
authors.lastname: Hirayama
authors.forename: Masaki
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Akio
authors.initials: A
authors.lastname: Koyama
mesh_terms: D001672:Biocompatible Materials / Q000592:standards; D002482:Cellulose / Q000031:analogs & derivatives*; D002845:Chromatography / Q000379:methods; D003093:Colitis, Ulcerative / Q000150:complications / Q000628:therapy; D006801:Humans; D017665:Hydroxyl Radical / Q000097:blood; D007937:Leukapheresis / Q000379:methods* / Q000592:standards; D018384:Oxidative Stress; D011093:Polyethylene Terephthalates; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
chemical_list: D001672:Biocompatible Materials; D011093:Polyethylene Terephthalates; D017392:Thiobarbituric Acid Reactive Substances; D017665:Hydroxyl Radical; C005062:acetylcellulose; D002482:Cellulose