Expression of junctional proteins in human platelets.
Platelets play a major role in thrombosis and hemostasis by binding the sub-endothelial matrix at sites of injury, but also participate in vascular pathologies such as atherosclerosis. Recently, junctional proteins like PECAM-I and JAM-family members have been recovered from platelets, therefore we examined what other junctional molecules may be present in platelets. We observed immunoreactivity for APC (147 kD), beta-catenin (92 kD), E-cadherin (120 and 84 kD) and occludin (70-85 kD) by western blotting. Additionally, beta-catenin, pan-reactive cadherins, E-cadherin and occludin were seen to partition with the triton insoluble cytoskeleton in platelets. These proteins were also found in a megakaryocyte (platelet precursor) line, MEG-01. Our data suggest that conventional junctional molecules are expressed in platelets and could possibly participate in aggregation, clot formation and wound healing.
Elrod
J W
JW
Department of Molecular and Cellular Physiology, Louisiana State University Medical Center, Shreveport, Louisiana 71130-3932, USA.
Park
J H
JH
Oshima
T
T
Sharp
C D
CD
Minagar
A
A
Alexander
J S
JS
D025601:Adenomatous Polyposis Coli Protein / Q000032:analysis; D001792:Blood Platelets / Q000737:chemistry* / Q000648:ultrastructure; D015153:Blotting, Western; D015820:Cadherins / Q000032:analysis; D015815:Cell Adhesion Molecules / Q000032:analysis*; D003598:Cytoskeletal Proteins / Q000032:analysis; D003599:Cytoskeleton / Q000378:metabolism; D006801:Humans; D007365:Intercellular Junctions / Q000737:chemistry*; D008565:Membrane Proteins / Q000032:analysis; D062793:Occludin; D011092:Polyethylene Glycols; D015534:Trans-Activators / Q000032:analysis; D051176:beta Catenin
D025601:Adenomatous Polyposis Coli Protein; C495270:CTNNB1 protein, human; D015820:Cadherins; D015815:Cell Adhesion Molecules; D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; C568985:OCLN protein, human; D062793:Occludin; D015534:Trans-Activators; D051176:beta Catenin; D011092:Polyethylene Glycols