Development of Linum flavum hairy root cultures for production of coniferin.
Linum flavum hairy roots were initiated from leaf discs using Agrobacterium rhizogenes strains LBA9402 and TR105 though two other strains, 15834 and A4, were relatively ineffective for induction. Significant variation in coniferin accumulation was observed between hairy root lines originating from different L. flavum seedlings and/or A. rhizogenes strains. Coniferin reached 58 mg g-1 dry wt by culturing the roots in Linsmaier and Skoog (LS) medium with 2,4-dichlorophenoxyacetic acid and naphthaleneacetic acid as growth regulators.
Lin
Han-wei
HW
School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia.
Kwok
Kian H
KH
Doran
Pauline M
PM
D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D018929:Cell Culture Techniques / Q000379:methods*; D002934:Cinnamates / Q000378:metabolism*; D003470:Culture Media; D019597:Flax / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism*; D012231:Rhizobium
D002934:Cinnamates; D003470:Culture Media; D009280:Naphthaleneacetic Acids; D010937:Plant Growth Regulators; D015084:2,4-Dichlorophenoxyacetic Acid; C016316:coniferin