title: [Intraoperative histological sections in obstetric brachial plexus lesions--comparison of macroscopic appearance, HE staining and toluidine blue staining].
abstract: In brachial plexus lesions and their revision, evaluation of nerve stumps is very important for the reconstructive strategy. We intraoperatively perform cryostat sections, Haematoxilin-Eosin (HE) stained, and compare the clinical appearance of the nerves to the microscopic results. Toluidine blue staining is later used to validate the structural details. Intraneural fibrosis can be traced safely with both staining methods, in root avulsions a histology is helpful, too. For more proximal, intraforaminal lesions semithin section stained with toluidine-blue are less informative than are HE-stained cryostat sections. In these lesions the clinical control by electrical stimulation and evoked potentials is superior.
authors.affiliation: Universität Witten-Herdecke/Helios-Klinikum Wuppertal-Plastische Chirurgie, Wuppertal. becker.pch@gmx.de
authors.forename: M
authors.initials: M
authors.lastname: Becker
authors.forename: G
authors.initials: G
authors.lastname: Ingianni
authors.forename: F
authors.initials: F
authors.lastname: Lassner
authors.forename: D
authors.initials: D
authors.lastname: Atkins
authors.forename: J M
authors.initials: JM
authors.lastname: Schröder
mesh_terms: D000328:Adult; D001720:Birth Injuries / Q000473:pathology / Q000601:surgery*; D001917:Brachial Plexus / Q000293:injuries* / Q000473:pathology / Q000601:surgery; D002648:Child; D002675:Child, Preschool; D004396:Coloring Agents; D004801:Eosine Yellowish-(YS); D005260:Female; D005355:Fibrosis; D005629:Frozen Sections; D006416:Hematoxylin; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008866:Microsurgery; D010291:Paresis / Q000473:pathology* / Q000601:surgery*; D011247:Pregnancy; D011843:Radiculopathy / Q000473:pathology / Q000601:surgery; D012680:Sensitivity and Specificity; D014048:Tolonium Chloride
chemical_list: D004396:Coloring Agents; D014048:Tolonium Chloride; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin