Determination of p-tyrosol and salidroside in three samples of Rhodiola crenulata and one of Rhodiola kirilowii by capillary zone electrophoresis.
A capillary zone electrophoresis (CZE) method has been developed for simultaneous assay of two bioactive components (p-tyrosol and salidroside) in Rhodiola crenulata and Rhodiola kirilowii for the first time. Those analytes were successfully separated within 15 min using 50 mmol L(-1) (pH 9.62) borate containing 30% methanol as running buffer. Regression equations revealed linear relationships (correlation coefficients 0.9998-0.9999) between peak area and concentration of the two analytes. The relative standard deviations ( RSD) of the migration times and the peak areas of two constituents ranged from 0.51 to 0.57% and from 0.65 to 1.17%, respectively, intra-day, and from 4.91 to 6.93% and from 3.51 to 5.33%, respectively, inter-day. The recoveries of two constituents ranged from 96.24 to 103.15%.
Cui
Shuya
S
National Key Laboratory of Applied Organic Chemistry, Department of Chemistry, Lanzhou University, 730000 Lanzhou, P.R. China.
Hu
Xiaoli
X
Chen
Xingguo
X
Hu
Zhide
Z
D002138:Calibration; D019075:Electrophoresis, Capillary / Q000379:methods*; D005960:Glucosides / Q000032:analysis*; D010636:Phenols / Q000032:analysis*; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000032:analysis*; D029771:Rhodiola / Q000737:chemistry*; D013045:Species Specificity
D005960:Glucosides; D010636:Phenols; C011867:4-hydroxyphenylethanol; C009172:rhodioloside; D010626:Phenylethyl Alcohol