title: The relationship between LDL oxidation and macrophage myeloperoxidase activity.
abstract: OBJECTIVE
  To explore low density lipoprotein (LDL) oxidation by macrophage myeloperoxidase (MPO) at molecular level.
  
  
  METHODS
  Using a mouse macrophage model, we examined the relationship between LDL oxidation and macrophage MPO by measuring macrophage MPO activity, LDL oxidation products, MPO gene expression and cellular orientation of LDL oxidation.
  
  
  RESULTS
  MPO gene expression increased to its maximum gradually when the concentration of LDL was increased, and then maintained at that level. NaN(3) inhibied the elevation of MPO activity and LDL oxidation, which was LDL concentration-dependent. After the composition of macrophage membrane was roughly analyzed, it was determined that the contents of MPO and LDL in 5% sucrose were 7.667 and 21 times higher than those in 10% sucrose, respectively.
  
  
  CONCLUSIONS
  LDL is attached to the "microdomain" of the macrophage membrane in which LDL is oxidized by MPO.
authors.affiliation: Department of Biochemistry, College of Medicine, Wuhan University, Wuhan 430071, China.
authors.forename: Junzhu
authors.initials: J
authors.lastname: Wu
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Li
authors.forename: Lida
authors.initials: L
authors.lastname: Chen
authors.forename: Laju
authors.initials: L
authors.lastname: Xia
authors.forename: Jialing
authors.initials: J
authors.lastname: Hong
mesh_terms: D000818:Animals; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000235:genetics / Q000378:metabolism*
chemical_list: D008077:Lipoproteins, LDL; D009195:Peroxidase