Suspension culture of hematopoietic stem cells in stirred bioreactors.
Hematopoietic stem cells have applications in bone marrow transplantations for the treatment of hematopoietic disorders. When murine hematopoietic stem cells were cultured in 50 ml stirred bioreactors for 14 d, stem-cell-antigen-1 positive cells (hematopoietic primitive progenitor cells) and long-term culture-initiating cells (hematopoietic stem cells) grew by 5-fold and 4-fold, respectively. These results show the possibility of growing hematopoietic stem cells using a stirred bioreactor.
Kwon
Joon
J
Department of Chemical Engineering, Hanyang University, Seoul, Korea.
Kim
Byung-Soo
BS
Kim
Mi-Jung
MJ
Park
Hong-Woo
HW
D000818:Animals; D019149:Bioreactors; D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D005240:Feasibility Studies; D005434:Flow Cytometry / Q000379:methods; D006412:Hematopoietic Stem Cells / Q000145:classification / Q000166:cytology* / Q000502:physiology*; D051379:Mice