Quantification of doxorubicin and validation of reversal effect of tea polyphenols on multidrug resistance in human carcinoma cells.
HPLC was used to analyze doxorubicin in multidrug-resistance (MDR) human carcinoma cells. This method is novel, simple, sensitive, linear, accurate and precise. The minimal detectable concentration is 0.2 microg ml(-1). The reversal effects of tea polyphenols on MDR are elucidated by this method. The results indicate that the tea polyphenol, (-)-epigallocatechin gallate, is a potential modulator of MDR.
Wei
Dongzhi
D
State Key Laboratory of Bioreactor Engineering, Institute of Biochemistry, East China University of Science and Technology, Shanghai 200237, PR China. dzhwei@ecust.edu.cn
Mei
Yuying
Y
Liu
Jianwen
J
D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004317:Doxorubicin / Q000032:analysis* / Q000494:pharmacology*; D018432:Drug Resistance, Multiple; D005419:Flavonoids; D006801:Humans; D009062:Mouth Neoplasms / Q000378:metabolism / Q000503:physiopathology; D010636:Phenols / Q000494:pharmacology*; D011108:Polymers / Q000494:pharmacology*; D013662:Tea / Q000737:chemistry; D014407:Tumor Cells, Cultured
D005419:Flavonoids; D010636:Phenols; D011108:Polymers; D013662:Tea; D004317:Doxorubicin; D002392:Catechin; C045651:epigallocatechin gallate