title: Combined effect of hydrogen peroxide induced oxidative stress and IL-1 alpha on IL-8 production in CaCo-2 cells (a human colon carcinoma cell line) and normal intestinal epithelial cells.
abstract: Oxidative stress, IL-1alpha, and IL-8 are known to contribute to mucosal inflammation of the gastrointestinal tract. We examined the IL-8 response after brief exposure to hydrogen peroxide induced oxidative stress in CaCo-2 cells (a human colon carcinoma cell line) and in human intestinal epithelial cells. In addition, we examined whether exposure to oxidative stress, followed by IL-1alpha, could modulate IL-8 production. A transient up-regulation of IL-8 mRNA expression was observed after hydrogen peroxide treatment. Hydrogen peroxide induced oxidative stress was also observed to promote IL-8 secretion. Exposure to hydrogen peroxide, followed by IL-1alpha, enhanced IL-8 production over that achieved with IL-1alpha alone. Thus, oxidative stress and IL-1alpha were observed to cooperatively enhance IL-8 production.
authors.affiliation: Department of Clinical Laboratory Medicine, Shiga University of Medical Science, Seta-tsukinowa, Ohtsu, Shiga, Japan. yamak@belle.shiga-med.ac.jp
authors.forename: Kazuo
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Ryoji
authors.initials: R
authors.lastname: Kushima
authors.forename: Osamu
authors.initials: O
authors.lastname: Kisaki
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Fujiyama
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Okabe
mesh_terms: D018938:Caco-2 Cells; D002460:Cell Line; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007375:Interleukin-1 / Q000096:biosynthesis*; D016209:Interleukin-8 / Q000096:biosynthesis*; D007413:Intestinal Mucosa / Q000166:cytology / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology
chemical_list: D007375:Interleukin-1; D016209:Interleukin-8; D006861:Hydrogen Peroxide