Heat stress affects oogenesis differently in wild-type Drosophila virilis and a mutant with altered juvenile hormone and 20-hydroxyecdysone levels.
The link between reproduction and environmental signals is poorly understood at the physiological, genetic and molecular levels. We describe a mutant strain of Drosophila virilis that has altered responses to heat stress. Heat stress in wild-type females results in oocyte maturation delays, degradation of early vitellogenic egg chambers, inhibition of yolk protein gene expression in follicle cells and accumulation of mature oocytes. The mutant females have increased levels of ecdysteroids and decreased juvenile hormone degradation, and show all of the heat-stress-induced reproductive effects observed in wild-type flies, without exposure to heat stress. During oogenesis in mutant females following heat stress there is an increase in early vitellogenic oocyte degradation and some degradation of late egg chambers. 20-Hydroxyecdysone levels, but not juvenile hormone degradation, change following heat stress in mutant females.
Gruntenko
N E
NE
Institute of Cytology and Genetics, SD RAS, Novosibirsk, Russia.
Bownes
M
M
Terashima
J
J
Sukhanova
M Zh
MZh
Raushenbach
I Yu
IY
D000818:Animals; D001562:Benzimidazoles / Q000378:metabolism; D004330:Drosophila / Q000235:genetics / Q000502:physiology*; D029721:Drosophila Proteins; D004441:Ecdysterone / Q000378:metabolism / Q000502:physiology*; D004527:Egg Proteins / Q000235:genetics / Q000378:metabolism; D005260:Female; D018882:Heat Stress Disorders / Q000503:physiopathology*; D017403:In Situ Hybridization; D007301:Insect Hormones / Q000235:genetics / Q000502:physiology; D007605:Juvenile Hormones / Q000235:genetics / Q000502:physiology*; D008297:Male; D008857:Microscopy, Interference; D009154:Mutation; D009866:Oogenesis / Q000502:physiology*; D010053:Ovary / Q000502:physiology
D001562:Benzimidazoles; D029721:Drosophila Proteins; D004527:Egg Proteins; D007301:Insect Hormones; D007605:Juvenile Hormones; C070799:YP3 protein, Drosophila; C447680:hoechst 32258; D004441:Ecdysterone