title: Short-term exposures to chronically toxic copper concentrations induce HSP70 proteins in midge larvae (Chironomus tentans).
abstract: In the present study, we investigated the induction of HSP70 proteins in Chironomus tentans in response to copper exposure. Larvae were exposed for 24 h to 0, 0.25, 0.5, 1.0 and 2.0 mgl(-1) copper, concentrations that were previously shown to cause growth reduction or mortality in 10 d bioassays. The induction of HSP70 was evaluated by Western blotting, and we observed a significant increase in HSP70 proteins over controls at all tested concentrations. The results suggest that short-term laboratory exposures to chronically toxic but acutely sublethal copper concentrations induce HSP70 proteins in C. tentans larvae.
authors.affiliation: Department of Biology, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
authors.forename: Natalie K
authors.initials: NK
authors.lastname: Karouna-Renier
authors.forename: Jonathan P
authors.initials: JP
authors.lastname: Zehr
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002683:Chironomidae / Q000502:physiology*; D003300:Copper / Q000494:pharmacology / Q000633:toxicity*; D004781:Environmental Exposure; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis / Q000096:biosynthesis*; D007814:Larva / Q000502:physiology
chemical_list: D015415:Biomarkers; D018840:HSP70 Heat-Shock Proteins; D003300:Copper