title: Molecular characterization and immunolocalization of Schistosoma mansoni ATP-diphosphohydrolase.
abstract: Schistosoma mansoni, a human parasite that constitutes a major health problem in developing countries, escapes from host defenses and survives in the human bloodstream. Here, we report the cloning of a S. mansoni ATP-diphosphohydrolase ortholog (SmATPDase1). Southern blots indicated that in S. mansoni it is a single-copy gene. RT-PCR revealed that SmATPDase1 is expressed in five stages of the parasite life cycle, namely cercaria, schistosomula, adults, eggs, and miracidia. Using confocal microscopy, SmATPDase1 protein was immunolocalized on the external surface in all stages, except eggs, being conspicuously present in adults. ATPDase, which is present on the outer surface of endothelial cells lining human blood vessels, has been implicated in thromboregulation by promoting ADP hydrolysis and inhibition of platelet aggregation. The presence of an ATPDase ortholog on the surface of S. mansoni suggests that the enzyme might play a role in the escape from host defenses that would involve platelet activation.
authors.affiliation: Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, 05508-900 São Paulo, SP, Brazil.
authors.forename: Ricardo
authors.initials: R
authors.lastname: DeMarco
authors.forename: Andre T
authors.initials: AT
authors.lastname: Kowaltowski
authors.forename: Renato A
authors.initials: RA
authors.lastname: Mortara
authors.forename: Sergio
authors.initials: S
authors.lastname: Verjovski-Almeida
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D001081:Apyrase / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D003001:Cloning, Molecular; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D010802:Phylogeny; D012550:Schistosoma mansoni / Q000166:cytology / Q000201:enzymology*; D017386:Sequence Homology, Amino Acid
chemical_list: D000909:Antibodies, Helminth; D008565:Membrane Proteins; D001081:Apyrase