title: [Construction of human papilloma virus type 18 E6E7 genes in adeno-associated virus expression vector and checking its activity for malignant transformation].
abstract: BACKGROUND
  To construct human papillomavirus type 18 (HPV18 E6E7) adeno-associated virus (AAV) for studying the role of HPV E6E7 in the development of human cancer.
  
  
  METHODS
  HPV18 E6E7 genes were inserted into adeno-associated virus expression vector and then infected 293 cell line. The expression of HPV18 E6E7 genes were confirmed by using RT-PCR/Southern blot assay.
  
  
  RESULTS
  There was HPV18 E6E7 genes in the malignantly transformed cell line. The 293TL cells compared with the parent cells transformed cells grew more rapidly, lost their contact inhibition and formed more and large colonies in soft agar.
  
  
  CONCLUSIONS
  HPV18 E6E7 AAV was successfully constructed and could induce malignant transformation. HPV18 E6E7 AAV can be use for studying the immortalization and malignant transformation of human normal epithelial cells.
authors.affiliation: Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Cen
authors.forename: Zhi-ping
authors.initials: ZP
authors.lastname: Teng
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhong-ying
authors.initials: ZY
authors.lastname: Shen
authors.forename: Jin-jie
authors.initials: JJ
authors.lastname: Xu
authors.forename: Bin
authors.initials: B
authors.lastname: Du
authors.forename: Yi
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D004279:DNA, Viral / Q000032:analysis; D004268:DNA-Binding Proteins; D000229:Dependovirus / Q000235:genetics*; D004847:Epithelial Cells / Q000166:cytology* / Q000821:virology; D005333:Fetus; D006801:Humans; D007668:Kidney / Q000166:cytology; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D027383:Papillomaviridae / Q000235:genetics; D016133:Polymerase Chain Reaction
chemical_list: D004279:DNA, Viral; D004268:DNA-Binding Proteins; C052603:E6 protein, Human papillomavirus type 18; C052633:E7 protein, Human papillomavirus type 18; D009856:Oncogene Proteins, Viral