[Molecular analysis of hantavirus isolated from Shandong province].
OBJECTIVE
To compare the molecular characteristics of hantaviruses isolated from Shandong province by using PCR typing and nucleotide sequencing.


METHODS
Total cellular RNA was extracted from hantaviruses infected Vero E6 cells, viral cDNA was amplified by polymerase chain reaction. PCR products of partial M Segments of 4 strains of hantaviruses were sequenced. Cross neutralization tests were performed.


RESULTS
Four strains of hantavirus isolated from Rottus in Shandong province were SEO like viruses. The homology between Shandong isolates and other SEO like viruses was high at both amino acid and nucleotide levels. The homology among those 4 strains of hantaviruses was 98%.


CONCLUSIONS
The SEO like hantaviruses were more conserved than other hantaviruses. The homology of SEO like hantaviruses isolated from Shandong province was as high as 98% at nucleotides level, though they were isolated at more than 10-year intervals.
Wang
Zhi-qiang
ZQ
Shandong Hygiene and Anti-epidemic Station, Jinan 250014, China.
Wang
Yu-lu
YL
Fu
Ji-hua
JH
Zhao
Ling
L
Sun
Chen-yan
CY
Zhang
Xue-qi
XQ
Zhang
Yan-xue
YX
Fan
Shu-zhen
SZ
Wang
Nan-da
ND
D000818:Animals; D001483:Base Sequence; D002681:China; D004279:DNA, Viral / Q000032:analysis*; D004197:Disease Reservoirs; D014644:Genetic Variation; D018063:Hantaan virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006480:Hemorrhagic Fever with Renal Syndrome / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid
D004279:DNA, Viral