Enhanced biosynthesis of polyhydroxyalkanoates in a mutant strain of Rhizobium meliloti.
Strains of Rhizobium spp. isolated from leguminous plants and standard strains accumulated 27% to 57% polyhydroxyalkanoate (PHA) of their cell biomass. Among these cultures, one strain of Rhizobium meliloti synthesized 10-30% more PHA than others and contained 3% hydroxyvalerate (HV) when grown on sucrose as carbon substrate. The occurrence of hydroxybutyrate (HB) and HV was confirmed by GC and 1H NMR analysis. Treatment of the culture with 4'-N-piperidinobutyl-2-chlorophenoxazine resulted in a mutant which synthesized up to 69%, PHA of the cell biomass with an improved yield of 11 to 47% under different carbon and nitrogen ratios, compared to the parent strain.
Lakshman
Kshama
K
Department of Food Microbiology, Central Food Technological Research Institute, Mysore 570 013, India.
Shamala
Tumkur Ramachandriah
TR
D002244:Carbon / Q000378:metabolism; D002264:Carboxylic Acids / Q000378:metabolism*; D007887:Fabaceae / Q000382:microbiology; D006885:Hydroxybutyrates / Q000378:metabolism*; D009154:Mutation; D009584:Nitrogen; D010421:Pentanoic Acids / Q000378:metabolism; D018517:Plant Roots / Q000382:microbiology; D011091:Polyesters / Q000378:metabolism*; D011108:Polymers / Q000378:metabolism; D011786:Quality Control; D016962:Sinorhizobium meliloti / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D013045:Species Specificity; D013395:Sucrose / Q000378:metabolism*; D000094122:Polyhydroxybutyrates
D002244:Carbon; D002264:Carboxylic Acids; D006885:Hydroxybutyrates; D009584:Nitrogen; D010421:Pentanoic Acids; D011091:Polyesters; D011108:Polymers; D013395:Sucrose; C013056:beta-hydroxyvaleric acid; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates