Mechanism of anticlastogenicity of Agaricus blazei Murill mushroom organic extracts in wild type CHO (K(1)) and repair deficient (xrs5) cells by chromosome aberration and sister chromatid exchange assays.
Agaricus blazei Murill is a medicinal mushroom native to Brazil. The present work assessed the clastogenic and anticlastogenic potential of organic extracts (ethanol and chloroform/methanol) from the lineage AB97/11 in chinese hamster CHO-K(1) (wild type) and CHO-xrs5 (repair deficient) cells using the chromosome aberration (CA) and sister chromatid exchange (SCE) assays. In these experimental conditions were observed: (a) anticlastogenic effect at concentrations of 0.06 and 0.09% of the EtOH extract and at the 0.03 and 0.06% concentrations of the C/MetOH extract in CHO-K(1); (b) absence of protector effect on CHO-xrs5 cells; and (c) absence of protector effect in the SCE assay. These results indicate that organic extracts of A. blazei lineage AB97/11 present bio-antimutagenic type protective activity.
Luiz
R C
RC
Departamento de Biologia Geral, Centro de Ciencias Biologicas (CCB), Universidade Estadual de Londrina, Campus Universitário, Cx. Postal 6001, CEP 86051-990 PR Londrina, Brazil.
Jordão
B Q
BQ
da Eira
A F
AF
Ribeiro
L R
LR
Mantovani
M S
MS
D000364:Agaricus / Q000737:chemistry*; D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D016466:CHO Cells; D002869:Chromosome Aberrations; D006224:Cricetinae; D009152:Mutagenicity Tests; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal; D012854:Sister Chromatid Exchange
D016587:Antimutagenic Agents; D010936:Plant Extracts