[Effects of ischemic reperfusion on the expression of cell adhesion molecules and monocyte chemotactic protein-1 and the protective role of MgSO4].
To investigate the effect of ischemic reperfusion(I-R) on the expression of P-selectin(Ps) of platelets, CD11b of leukocytes, monocyte chemotactic protein-1(MCP-1) mRNA of myocardial tissue and the protective role of MgSO(4).



Ischemic reperfusion rat model was established in the experiments. All animals were randomly divided into 3 groups  sham group, I-R group and Mg(2+) group. The expression of Ps of platelets and CD11b of leukocytes was determined by flowcytometry and the expression of MCP-1 mRNA of myocardial tissue was measured by RT-PCR admission and at 5, 30, 60, 180, 360 min of reperfusion (R5, R30, R60, R180, R360).



The expression of Ps of platelets in I-R group began to rise at R5 and peaked at R60  P<0.01); the expression of CD11b of leukocytes was significantly higher than that of the control groups  P<0.01 and P<0.05)  the expression of MCP-1 mRNA of myocardial tissue began to rise at R60,reaching the highest at R360  P<0.01)  the concentration of Ca(2+) of the serum was significantly higher than that of the control groups  P<0.01 and P<0.05). MgSO(4) inhibited the expression of Ps of platelets and MCP-1 mRNA of myocardial tissue and decreased serum Ca(2+)concentration, but did not affect the expression of CD11b of leukocytes.



I-R can increase the expression of cell adhesion molecules and cell chemotactic factor. MgSO(4) may protect myocardial tissue from ischemic reperfusion injury by inhibiting the expression of Ps of platelets and MCP-1 mRNA of myocardial tissue and decreasing the concentration of Ca(2+) of the serum.
Ying
Shu-qin
SQ
Department of Cardiology, The Second Affiliated Hospital, College of Medical Sciences, Zhejiang University, Hangzhou 310009  China.
Xu
Geng
G
Shan
Jiang
J
D000818:Animals; D039481:CD11b Antigen / Q000235:genetics*; D002118:Calcium / Q000097:blood; D018932:Chemokine CCL2 / Q000235:genetics*; D015870:Gene Expression; D008278:Magnesium Sulfate / Q000627:therapeutic use*; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000517:prevention & control*; D019007:P-Selectin / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley
D039481:CD11b Antigen; C491766:Ccl2 protein, rat; D018932:Chemokine CCL2; D019007:P-Selectin; D012333:RNA, Messenger; D008278:Magnesium Sulfate; D002118:Calcium