Growth temperature downshift induces antioxidant response in Saccharomyces cerevisiae.
A rapid downshift in the growth temperature of Saccharomyces cerevisiae from 30 to 10 degrees C resulted in an increase in transcript levels of the antioxidation genes SOD1 [encoding Cu-Zn superoxide dismutase (SOD)], CTT1 (encoding catalase T), and GSH1 (encoding gamma-glutamylcysteine synthetase). The cellular activities of SOD and catalase were also increased, indicating that the temperature downshift caused an antioxidant response. In support of this, a simultaneous increase in the intracellular level of H(2)O(2) was observed. The level of YAP1 mRNA, encoding a transcription factor critical for the oxidative stress response in this yeast, was also increased by the temperature downshift. However, deletion of YAP1 did not reduce the elevated mRNA levels of the antioxidant genes. This suggests that the temperature downshift-induced increase in the mRNA level of anti-oxidant genes is YAP1-independent.
Zhang
Lei
L
Laboratory of Cellular Genetics, Department of Biotechnology, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, Japan.
Onda
Kouki
K
Imai
Ryozo
R
Fukuda
Ryouichi
R
Horiuchi
Hiroyuki
H
Ohta
Akinori
A
D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000235:genetics / Q000378:metabolism*; D003080:Cold Temperature; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D005721:Glutamate-Cysteine Ligase / Q000235:genetics / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D016877:Oxidants / Q000378:metabolism; D018384:Oxidative Stress; D012333:RNA, Messenger / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D000975:Antioxidants; D005656:Fungal Proteins; D016877:Oxidants; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C086677:YAP1 protein, S cerevisiae; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase; D005721:Glutamate-Cysteine Ligase