title: Phospholipids from Bombycis corpus and their neurotrophic effects.
abstract: Three phospholipids (4-6) and three aromatic amines (1-3) were obtained from the methanol extract of Bombycis corpus. Based on spectral data, their structures have been elucidated as nicotiamide (1), cytidine (2), adenine (3), 1-O-(9Z-octadecenoyl)-2-O-(8Z,11Z-octadecadienoyl) sn-glycero-3-phosphorylcholine (4), 1,2-di-O-hexadecanoyl-sn-glycero-3-phosphorylcholine (5) and 1,2-di-O-9Z-octadecenoyl-sn-glycero-3-phosphorylcholine (6). We examined the effects of compounds on synthesis of NGF in cultured astrocytes. By RT-PCR analysis, expresison of NGF mRNA in astrocytes cultured in serum-starvation increased after the addition of phospholipid (10 microM). The NGF content in the culture medium was significantly increased by compound 5, compared with the control value. These results suggest that three phospholipid compounds isolated from the methanol extract of Bombycis corpus may exert neurotrophic effects by stimulation of NGF synthesis in astrocytes.
authors.affiliation: College of Pharmacy, SungKyunKwan University, Suwon 440-746, Korea.
authors.forename: Hak Cheol
authors.initials: HC
authors.lastname: Kwon
authors.forename: I Yeon
authors.initials: IY
authors.lastname: Jung
authors.forename: Se Yeon
authors.initials: SY
authors.lastname: Cho
authors.forename: Ock Ryun
authors.initials: OR
authors.lastname: Cho
authors.forename: Min Cheol
authors.initials: MC
authors.lastname: Yang
authors.forename: Sung Ok
authors.initials: SO
authors.lastname: Lee
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Hur
authors.forename: Sun Yeou
authors.initials: SY
authors.lastname: Kim
authors.forename: Jong Beom
authors.initials: JB
authors.lastname: Yang
authors.forename: Kang Ro
authors.initials: KR
authors.lastname: Lee
mesh_terms: D000818:Animals; D009414:Nerve Growth Factors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D016716:PC12 Cells; D010743:Phospholipids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D028321:Plant Preparations / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats
chemical_list: D009414:Nerve Growth Factors; D010743:Phospholipids; D010936:Plant Extracts; D028321:Plant Preparations