Production of ajmalicine and ajmaline in hairy root cultures of Rauvolfia micrantha Hook f., a rare and endemic medicinal plant.
Hairy roots of Rauvolfia micrantha were induced from hypocotyl explants of 2-3 weeks old aseptic seedlings using Agrobacterium rhizogenes ATCC 15834. Hairy roots grown in half-strength Murashige & Skoog (MS) medium with 0.2 mg indole 3-butyric acid l-1 and 0.1 mg alpha-naphthaleneacetic acid l-1 produced more ajmaline (0.01 mg g-1 dry wt) and ajmalicine (0.006 mg g-1 dry wt) than roots grown in auxin-free medium. Ajmaline (0.003 mg g-1 dry wt) and ajmalicine (0.0007 mg g-1 dry wt) were also produced in normal root cultures. This is the first report of production of ajmaline and ajmalicine in hairy root cultures of Rauvolfia micrantha.
Sudha
C G
CG
Plant Biotechnology Division, Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapuram 695 562, Kerala, India.
Obul Reddy
B
B
Ravishankar
G A
GA
Seeni
S
S
D000404:Ajmaline / Q000096:biosynthesis*; D002454:Cell Differentiation; D046508:Culture Techniques / Q000379:methods; D007210:Indoleacetic Acids / Q000494:pharmacology; D018517:Plant Roots / Q000166:cytology / Q000254:growth & development / Q000378:metabolism* / Q000382:microbiology; D010946:Plants, Medicinal / Q000166:cytology / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology; D011926:Rauwolfia / Q000166:cytology / Q000254:growth & development / Q000378:metabolism* / Q000382:microbiology; D012231:Rhizobium / Q000502:physiology; D046948:Secologanin Tryptamine Alkaloids; D015016:Yohimbine / Q000031:analogs & derivatives* / Q000378:metabolism*
D007210:Indoleacetic Acids; D046948:Secologanin Tryptamine Alkaloids; D000404:Ajmaline; D015016:Yohimbine; C005709:raubasine