Involvement of beta-arrestin-2 in modulation of the spinal antinociception induced by mu-opioid receptor agonists in the mouse.
Beta-arrestins have been suggested to regulate mu-, delta-, and kappa-opioid receptor-mediated responses. In the present study, we examined the effects of pretreatment with beta-arrestin-2 antibody on tail-flick inhibition induced by opioid receptor agonists in the mouse spinal cord. Intrathecal (i.t.) pretreatment with beta-arrestin-2 antibody potentiated the antinociception induced by i.t.-administered mu-opioid receptor agonists [D-Ala(2),NMePhe(4),Gly-ol(5)]enkephalin (DAMGO) and endomorphin-1, but not endomorphin-2, the delta-opioid receptor agonist [D-Ala(2)]deltorphin II or the kappa-opioid receptor agonist U50,488H. The present result suggests that beta-arrestin-2 may tonically down-regulate a selected population of mu-opioid receptors activated by endomorphin-1 or DAMGO in the mouse spinal cord.
Ohsawa
Masahiro
M
Department of Anesthesiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
Mizoguchi
Hirokazu
H
Narita
Minoru
M
Nagase
Hiroshi
H
Dun
Nae J
NJ
Tseng
Leon F
LF
D000700:Analgesics / Q000494:pharmacology*; D000701:Analgesics, Opioid / Q000494:pharmacology; D000818:Animals; D000906:Antibodies / Q000494:pharmacology / Q000502:physiology; D019390:Arrestins / Q000494:pharmacology* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010147:Pain Measurement / Q000187:drug effects* / Q000379:methods; D017450:Receptors, Opioid, mu / Q000819:agonists* / Q000502:physiology; D013116:Spinal Cord / Q000187:drug effects* / Q000502:physiology; D000071560:beta-Arrestin 2; D000071557:beta-Arrestins
D000700:Analgesics; D000701:Analgesics, Opioid; D000906:Antibodies; C000605258:Arrb2 protein, mouse; D019390:Arrestins; D017450:Receptors, Opioid, mu; D000071560:beta-Arrestin 2; D000071557:beta-Arrestins