Sterols and triterpenes in cell culture of Hyssopus officinalis L.
Cell suspension cultures from hypocotyl-derived callus of Hyssopus officinalis were found to produce two sterols i. e. beta-sitosterol (1) and stigmasterol (2), as well as several known pentacyclic triterpenes with an oleanene and ursene skeleton. The triterpenes were identified as oleanolic acid (3), ursolic acid (4), 2alpha,3beta-dihydroxyolean-12-en-28-oic acid (5), 2alpha,3beta-dihydroxyurs-12-en-28-oic acid (6), 2alpha,3beta,24-trihydroxyolean-12-en-28-oic acid (7), and 2alpha,3beta,24-trihydroxyurs-12-en-28-oic acid (8). Compounds 5-8 were isolated as their acetates (6, 8) or bromolactone acetates (5, 7).
Skrzypek
Zuzanna
Z
Laboratory of Biology and Pharmaceutical Botany, Medical University, Muszynskiego 1, 90-151-Lódź, Poland. botanika@pharm.am.lodz.pl
Wysokińska
Halina
H
D018929:Cell Culture Techniques; D002855:Chromatography, Thin Layer; D019686:Lamiaceae / Q000737:chemistry* / Q000166:cytology; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008968:Molecular Conformation; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry*; D013261:Sterols / Q000737:chemistry* / Q000302:isolation & purification; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification
C575836:2,3,24-trihydroxyolean-12-en-28-oic acid; D010936:Plant Extracts; D013261:Sterols; D014315:Triterpenes; D009828:Oleanolic Acid