Effects of method and MRI slice thickness on entorhinal cortex volumetry.
We assessed the effect of the method of analysis and the MRI slice thickness on entorhinal cortex volumetry. A T1 gradient echo 3D volumetric acquisition was reformatted into different slice thickness and analyzed by edge-tracing. We performed two different forms of analysis of images with 3 mm slice thickness: edge-tracing and pixel by pixel. There was difference among the volumes obtained from different slice thickness (p < 0.001), and also difference between the two different methods of analysis (p < 0.05). The use of thick slices is time saving, but volumes are linearly increased; different methods of segmentation also yields different values. The form of volumetric analysis of the entorhinal cortex should be evaluated in advance to prevent false estimates in longitudinal studies.
Bonilha
Leonardo
L
Laboratory of Neuroimaging, State University of Campinas, Campinas, SP, Brazil. Ibonilha@unicamp.br
Kobayashi
Eliane
E
Cendes
Fernando
F
Li
Li M
LM
D000328:Adult; D000704:Analysis of Variance; D001931:Brain Mapping; D018728:Entorhinal Cortex / Q000033:anatomy & histology* / Q000378:metabolism; D005260:Female; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation / Q000379:methods; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D008875:Middle Aged