title: Enzymatic determination of pyrophosphate in urine by flow methods.
abstract: Two flow methods for the enzymatic determination of pyrophosphate are described that are used to diminish the consumption of reagents. One method is based on the use of an open-close circuit with manual injection using a syringe. The other is a sequential injection method. The analytical features of both methods are: a linear range of 0.4 - 20 mg L(-1), an LOD of 0.38 mg L(-1), and a CV of 2.0% for the sequential injection method, and a linear range of 0.3 - 15 mg L(-1), an LOD of 0.29 mg L(-1), and CV of 2.2% for the open-close circuit method. The methods were applied to the determination of pyrophosphate in urine. The pyrophosphate concentration determined in urine samples varied from 1.26 to 6.67 mg L(-1).
authors.affiliation: Department of Chemistry, University of Balearic Islands, E 07071 Palma de Mallorca, Spain.
authors.forename: Bartolomé M
authors.initials: BM
authors.lastname: Simonet
authors.forename: Félix
authors.initials: F
authors.lastname: Grases
authors.forename: Juan G
authors.initials: JG
authors.lastname: March
mesh_terms: D011756:Diphosphates / Q000378:metabolism* / Q000652:urine*; D004798:Enzymes / Q000378:metabolism; D017022:Flow Injection Analysis / Q000379:methods*; D006801:Humans
chemical_list: D011756:Diphosphates; D004798:Enzymes