title: Virulence of recent and former classical swine fever virus isolates evaluated by their clinical and pathological signs.
abstract: The clinical diagnosis of classical swine fever (CSF) still caused problems to the veterinarians during the last decade. The primary CSF outbreak was often detected too late and, meanwhile, the virus had spread. Consequently, the recent classical swine fever virus isolates (CSFV) were suspected to be of low virulence. The purpose of the study was to quantify the virulence of four recent CSFV by evaluating the clinical and pathological signs caused by different CSFV. Pigs of the same breed and age group were inoculated intranasally with CSFV from recent epidemics in European Union (EU) member states. The CSFV used are registered in the data base of the EU Reference Laboratory for CSF and belong to different genotypes: 2.1, 2.2 and 2.3 respectively. Clinical signs of CSF were evaluated by using a score system suggested previously (Mittelholzer et al., 2000: Vet. Microbiol. 74, 293). For the evaluation of pathological lesions, a new pathological score was introduced. The four CSFV tested here were classified as moderately virulent in general, although one CSFV may cause different clinical courses, ranging from highly virulent to avirulent. This indicates the importance of additional factors in the host animal for virulence. Differences in the clinical and pathological signs between these four recent CSFV were rather minor, emphasizing that the genetic typing of CSFV is absolutely essential. Differences towards former CSFV (e.g. reference virus strain Alfort 187) were more pronounced, especially regarding the onset and duration of the disease, the occurrence of skin haemorrhages and pathological lesions of kidney, subcutis and serosae. It is concluded that clinical diagnosis of CSF is rather difficult in pigs up to 14 days post-CSFV infection using these four CSFV, emphasizing the need for careful differential diagnosis and the laboratory investigation for CSF at an early stage.
authors.affiliation: EU Reference Laboratory for CSF, Institute of Virology, School of Veterinary Medicine, Buenteweg 17, 30559 Hannover, Germany. gundula_niesmann@yahoo.de
authors.forename: G
authors.initials: G
authors.lastname: Floegel-Niesmann
authors.forename: C
authors.initials: C
authors.lastname: Bunzenthal
authors.forename: S
authors.initials: S
authors.lastname: Fischer
authors.forename: V
authors.initials: V
authors.lastname: Moennig
mesh_terms: D000818:Animals; D006691:Classical Swine Fever / Q000453:epidemiology* / Q000209:etiology / Q000473:pathology / Q000821:virology*; D006692:Classical Swine Fever Virus / Q000472:pathogenicity*; D004196:Disease Outbreaks / Q000662:veterinary*; D005858:Germany / Q000453:epidemiology; D013552:Swine / Q000235:genetics