Two new antifungal saponins from the Tibetan herbal medicine Clematis tangutica.
Bioassay-guided fractionation of the ethanol extract of the aerial parts of Clematis tangutica led to the isolation of two new antifungal triterpene saponins. Their structures were determined to be 3- O-alpha- L-arabinopyranosyl hederagenin 28- O-alpha- L-rhamnopyranosyl ester ( 1) and 3- O-beta- D-glucopyranosyl-(1--> 4)-alpha- L-arabinopyranosyl hederagenin 28- O-alpha- L-rhamnopyranosyl ester ( 2) on the basis of spectral data and chemical evidence. Inhibitory activities of the two saponins against seven fungal strains were evaluated. Compounds 1 and 2 showed evident antifungal activity (MIA approximately 2.5 micrograms/disc) against Saccharomyces cerevisiae, similar to the positive control amphotericin B and ordinary activities (MIA approximately 10 micrograms/disc) against Penicillium avellaneum UC-4376, Candida glabrata, Trichosporon beigelii and Pyricularia oryzae. Compound 2 is a better antifungal agent than compound 1 against most of the fungal strains that were tested.
Du
Zhizhi
Z
The State Key Laboratory of Phytochemistry and Plant Resources in West China, P. R. China.
Zhu
Na
N
Ze-Ren-Wang-Mu
Na
N
Shen
Yuemao
Y
D000935:Antifungal Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D041221:Candida glabrata / Q000187:drug effects; D039324:Clematis; D005658:Fungi / Q000187:drug effects*; D006801:Humans; D008519:Medicine, Traditional; D008826:Microbial Sensitivity Tests; D010407:Penicillium / Q000187:drug effects; D008517:Phytotherapy; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D012441:Saccharomyces cerevisiae / Q000187:drug effects; D012503:Saponins / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D018609:Tibet; D014250:Trichosporon / Q000187:drug effects
D000935:Antifungal Agents; D010936:Plant Extracts; D012503:Saponins