title: Reduced viscosity polymer matrices for microchip electrophoresis of double-stranded DNA.
abstract: On a polymethylmethacrylate (PMMA) microchip, double-stranded DNA fragments with a wide size range from 50 bp to 20 kbp were separated by two polymer solutions. One was a hydroxypropylmethylcellulose-4000 (HPMC-4000) solution of 1.3% (w/v) to separate fragments below 590 bp, and another was a mixed four molecular weight poly(ethylene oxide) solution at a total concentration of 0.1% to separate fragments above 520 bp. The widths at half height (wh) of the fragments had a good relationship with their migration times (tR) in both polymer solutions. Such a relationship was suitable for obtaining the wh values of unresolved peaks, calculating the resolution of two adjacent fragments, and optimizing microchip separation matrices. Based on the relativity, a low viscosity medium containing 2% HPMC-50 and 8% glucose was optimized for high-performance separation of a phiX174 HaeIII restriction fragment digest.
authors.affiliation: Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokushima, CREST, Japan Science and Technology, Shomachi, Tokushima 770-8505, Japan.
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
authors.forename: Mohammad
authors.initials: M
authors.lastname: Jabasini
authors.forename: Shaoqian
authors.initials: S
authors.lastname: Liu
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Baba
mesh_terms: D004247:DNA / Q000032:analysis*; D004586:Electrophoresis / Q000379:methods; D006801:Humans; D008832:Microchemistry / Q000295:instrumentation; D011108:Polymers
chemical_list: D011108:Polymers; D004247:DNA