Use of tuftsin bearing nystatin liposomes against an isolate of Candida albicans showing less in vivo susceptibility to amphotericin B.
In the present study, we evaluated tuftsin bearing nystatin liposomes for their potential against an isolate of Candida albicans (C. albicans) showing less in vivo susceptibility to amphotericin B (Amp B). The liposomised-Amp B in higher doses was found to be effective in elimination of less susceptible strain of C. albicans (C. albicans JMCR) in Balb/c mice, but may not be recommended due to toxicity constraints. On the other hand, liposomal nystatin was shown to possess higher efficacy as compared to that of Amp B, and was pertinent in treatment of C. albicans JMCR strain. The data of present work reveals that the incorporation of nystatin in tuftsin-bearing-liposomes results in a significant increase in its efficacy against experimental murine candidiasis. Interestingly, the pre-treatment of animals with liposomised-tuftsin prior to challenge with C. albicans infection was more effective in elimination of the pathogen from host and shows an advantage in prophylactic perspectives.
Khan
Masood A
MA
Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India.
Syed
Faisal M
FM
Nasti
H T
HT
Saima Dagger
K
K
Haq
W
W
Shehbaz
A
A
Owais
M
M
D000276:Adjuvants, Immunologic / Q000008:administration & dosage* / Q000737:chemistry / Q000627:therapeutic use; D000666:Amphotericin B / Q000008:administration & dosage* / Q000737:chemistry / Q000627:therapeutic use; D000818:Animals; D019072:Antibiotic Prophylaxis; D000935:Antifungal Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000627:therapeutic use; D002176:Candida albicans / Q000187:drug effects; D002177:Candidiasis / Q000188:drug therapy* / Q000517:prevention & control; D002784:Cholesterol; D002851:Chromatography, High Pressure Liquid; D005260:Female; D008081:Liposomes; D051379:Mice; D008807:Mice, Inbred BALB C; D009761:Nystatin / Q000008:administration & dosage* / Q000737:chemistry / Q000627:therapeutic use; D010713:Phosphatidylcholines; D014405:Tuftsin / Q000008:administration & dosage* / Q000737:chemistry / Q000627:therapeutic use
D000276:Adjuvants, Immunologic; D000935:Antifungal Agents; D008081:Liposomes; D010713:Phosphatidylcholines; D009761:Nystatin; D000666:Amphotericin B; D002784:Cholesterol; D014405:Tuftsin