Multispectral laser-induced fluorescence imaging system for large biological samples.
A laser-induced fluorescence imaging system developed to capture multispectral fluorescence emission images simultaneously from a relatively large target object is described. With an expanded, 355-nm Nd:YAG laser as the excitation source, the system captures fluorescence emission images in the blue, green, red, and far-red regions of the spectrum centered at 450, 550, 678, and 730 nm, respectively, from a 30-cm-diameter target area in ambient light. Images of apples and of pork meat artificially contaminated with diluted animal feces have demonstrated the versatility of fluorescence imaging techniques for potential applications in food safety inspection. Regions of contamination, including sites that were not readily visible to the human eye, could easily be identified from the images.
Kim
Moon S
MS
Instrumentation and Sensing Laboratory, U.S. Department of Agriculture Agricultural Research Service, Building 303, Powder Mill Road, Beltsville, Maryland 20705, USA. kimm@ba.ars.usda.gov
Lefcourt
Alan M
AM
Chen
Yud-Ren
YR
D000818:Animals; D002417:Cattle; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005243:Feces / Q000737:chemistry*; D005506:Food Contamination / Q000032:analysis*; D005638:Fruit / Q000737:chemistry; D007834:Lasers; D027845:Malus / Q000737:chemistry; D008460:Meat / Q000032:analysis; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods*