Isolation and characterization of a transposon mutant of Pseudomonas aeruginosa affecting uptake of dibenzothiophene in n-tetradecane.
OBJECTIVE
Isolation and characterization of a transposon mutant of Pseudomonas aeruginosa affecting the uptake of dibenzothiophene (DBT) in n-tetradecane (n-TD).


RESULTS
The dsz desulphurization gene cluster from Rhodococcus erythropolis KA2-5-1 was transferred to the chromosome of P. aeruginosa NCIMB9571 using a transposon vector. A recombinant (named PARM1) was obtained which was able to desulphurize DBT in water, but not in n-TD.


CONCLUSIONS
PARM1 is a mutant deficient in a DBT transport system operational in n-TD. This transport system is independent of rhamnolipids and of the n-alkane transport system.


CONCLUSIONS
Pseudomonas aeruginosa NCIMB9571 seems to have a specific system of transporting hydrophobic compounds such as DBT in oil.
Noda
K
K
Bio-Refining Process Laboratory, Japan Cooperation Center, Petroleum, Shizuoka, Japan. nodake@brpl.jccp.or.jp
Watanabe
K
K
Maruhashi
K
K
D000473:Alkanes; D001693:Biological Transport, Active / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics; D005798:Genes, Bacterial; D005810:Multigene Family; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000378:metabolism*; D011995:Recombination, Genetic; D012240:Rhodococcus / Q000235:genetics; D012997:Solvents; D013876:Thiophenes / Q000493:pharmacokinetics*; D014867:Water
D000473:Alkanes; D004251:DNA Transposable Elements; D012997:Solvents; D013876:Thiophenes; C024713:n-tetradecane; D014867:Water; C016366:dibenzothiophene