[Primary study on the electrophoresis atlas of the PCR products of the internal transcribed spacerl regions of the rRNA gene from home-planted and wild Gentiana macrophylla, G. straminea].
OBJECTIVE
To analyse the electrophoresis atlas of the PCR amplified products of the internal transcribed spacerl regions of the rRNA gene in DNA extracted from home-planted and wild Gentiana macrophylla, G. straminea in Gansu.


METHODS
With synthetic peculiar PCR primer, internal transcribed spacerl sequences of rRNA gene were amplified in DNA extracted from home-planted and wild Gentiana macrophylla, G. straminea. And then the electrophoretic atlas of nPCR amplified products on the agar sugar gel were analysed.


RESULTS
The electrophoresis atlas showed that the lengths of the internal transcribed spacerl regions of the rRNA gene in DNA from Gentiana macrophylla, G. straminea had the same region 360 bp. The length had enough genetic information to analyse base sequence.


CONCLUSIONS
The PCR amplified products electrophoretic atlas of internal transcribed spacerl regions of the rRNA gene in DNA from Gentiana macrophylla, G. straminea can be used as one of signs for identification on molecule level.
Ji
Keping
K
Gansu College of Traditional Chinese Medicine, Lanzhou 730000.
Liu
Lisha
L
Zhang
Xiling
X
Wang
Lan
L
D018744:DNA, Plant / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D020459:Genes, rRNA; D030012:Gentiana / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D018517:Plant Roots / Q000235:genetics; D010946:Plants, Medicinal / Q000235:genetics*; D016133:Polymerase Chain Reaction; D013045:Species Specificity
D018744:DNA, Plant