Multi-element speciation of metalloproteins in fish tissue using size-exclusion chromatography coupled "on-line" with ICP-isotope dilution-time-of-flight-mass spectrometry.
An on-line isotope dilution (ID) method in combination with the coupling of size-exclusion high-performance liquid chromatography (SE-HPLC) to an axial inductively coupled plasma time-of-flight mass spectrometer (ICP-TOFMS) was applied to the quantitative speciation of Cu, Zn and Cd in carp and eel cytosols. Quantitative information on the distribution of essential and toxic metals between the different molecular weight fractions in kidney cytosols of control and Cd exposed carp was obtained with species-unspecific spiking by post-column addition of the enriched isotopes 65Cu, 67Zn and 106Cd. The isotope ratios 63Cu/65Cu, 64Zn/67Zn and 114Cd/106Cd, were monitored by ICP-TOFMS in transient signal mode. The determination of the total cytosolic element concentration was performed by flow injection conventional nebulization ICP-TOFMS. Speciation differences were encountered between control and Cd-stressed carp, indicating that quantitative speciation studies might be useful for ecotoxicological and biomonitoring purposes. Application of this methodology to quantitation of metals associated with metallothioneins (MT) in European eel, sampled at three different sites in Flanders, Belgium was also addressed. A significant increase of the amount of Cd, Cu and Zn bound to hepatic MT was observed with the increase of the total metal and MT concentrations of hepatic eel tissue.
Goenaga Infante
Heidi
H
University of Antwerp (UIA), Micro and Trace Analysis Centre, Universiteitsplein 1, B-2610 Wilrijk, Belgium.
Van Campenhout
Karen
K
Schaumlöffel
Dirk
D
Blust
Ronny
R
Adams
Freddy C
FC
D000818:Animals; D002104:Cadmium / Q000032:analysis; D002347:Carps / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003300:Copper / Q000032:analysis; D003600:Cytosol / Q000737:chemistry*; D004524:Eels / Q000378:metabolism; D004784:Environmental Monitoring / Q000379:methods*; D005399:Fishes / Q000378:metabolism*; D007668:Kidney / Q000648:ultrastructure*; D013058:Mass Spectrometry / Q000379:methods; D008667:Metalloproteins / Q000032:analysis*; D015032:Zinc / Q000032:analysis
D008667:Metalloproteins; D002104:Cadmium; D003300:Copper; D015032:Zinc