[Detection of hantaan virus from gamasid mite and chigger mite by molecular biological methods].
OBJECTIVE
To study the proliferation and location of hantaan virus (HV) in gamasid mites and chigger mites.


METHODS
HV RNA in gamasid mites and chigger mites were detected by reverse transcription, polymerase chain reaction (RT- PCR) and in situ hybridization.


RESULTS
The smallest quantity of mite from which HV RNA could be detected was 5 mites group. The titers of -and proliferated in mites HV RNA could be found in ovary cells and dug cells of gamasid mites and chigger mites by in situ hybridization.


CONCLUSIONS
The results showed that HV could be trans-stadially transmitted and proliferated in mites, and HV always located in ovary and dug organs of mites. These results provide direct evidence at molecular level for the role of gamasid mites and chigger mites as vectors in transmission of HV.
Zhang
Yun
Y
Institute of Military Medicine, Nanjing Command of PLA, Nanjing 210002, China.
Zhu
Jin
J
Deng
Xiao-zhao
XZ
Wu
Guang-hua
GH
Wang
Jing-jun
JJ
Zhang
Jia-ju
JJ
Xing
Ai-hua
AH
Wu
Jian-wei
JW
D000818:Animals; D001096:Arachnid Vectors; D002522:Chlorocebus aethiops; D005260:Female; D018063:Hantaan virus / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D006801:Humans; D017403:In Situ Hybridization; D007814:Larva / Q000821:virology; D008925:Mites / Q000821:virology*; D009758:Nymph / Q000821:virology; D010053:Ovary; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012367:RNA, Viral