title: Biological characteristics of cell lines of human dental alveolus.
abstract: OBJECTIVE
  To investigate the biological characteristics of cell lines of healthy and diseased human dental alveoli.
  
  
  METHODS
  Primary cell lines from either healthy or diseased human dental alveoli were obtained. Two cell lines, H-258 and H-171 derived from healthy and diseased human tissues respectively, were selected for morphological study and research on their growth and aging, using cell counting, and histochemical and immunohistochemical staining.
  
  
  RESULTS
  Primary cell lines were successfully established from innormal dental alveoli. After freezing and thawing for three times, cell growth was continued and no morphological alterations were observed. The doubling time was 53.4 hours and mean division index (MDI) was 4 per thousand. Cells were kept normal after twenty generations with no obvious reduction of doubling time and MDI. Of twenty-six primary cell lines derived from healthy human dental alveoli, only three cell lines achieved generation. After freezing and thawing for twice, cultured cells were still alive at a decreased growth speed, with doubling time of 85.9 hours and MDI of 3 per thousand. Both cell lines, H-171 and H-258, shared the characteristics of osteoblast.
  
  
  CONCLUSIONS
  Primary cell lines of diseased human dental alveoli show greater growth potential. All cell lines of dental alveoli share characteristics of osteoblast. The technique we developed may be put into practice for the treatment of abnormal dental alveoli.
authors.affiliation: Department of Stomatology, Chaoyang Hospital, Capital University of Medical Sciences, Beijing 100020, China.
authors.forename: Shizhang
authors.initials: S
authors.lastname: Chen
authors.forename: Jingxiang
authors.initials: J
authors.lastname: Huang
authors.forename: Mingxue
authors.initials: M
authors.lastname: Sun
authors.forename: Bin
authors.initials: B
authors.lastname: Zhao
mesh_terms: D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D006801:Humans; D020390:Tooth Socket / Q000166:cytology*