title: Expression of NHERF-1, NHERF-2, PDGFR-alpha, and PDGFR-beta in normal human kidneys and in renal transplant rejection.
abstract: Sodium-hydrogen exchanger regulatory factor-1 and -2 (NHERF-1 and NHERF-2) are adaptor proteins that regulate renal electrolyte transport and interact with the platelet-derived growth factor receptors (PDGFR). The distribution of the NHERF proteins and PDGFR was studied in normal human kidneys and in renal transplant rejection using immunocytochemistry. In normal kidneys, NHERF-1 was detected in proximal tubules. NHERF-2 was detected in glomeruli, peritubular capillaries, and collecting duct principal cells. NHERF-2 was also weakly detected in the proximal tubule. PDGFR-beta was detected in glomeruli but not in tubules while PDGFR-alpha was detected in renal tubules and minimally in glomeruli. Acute and chronic transplant rejection was associated with increased expression of PDGFR-alpha in tubules and expression in the glomeruli. PDGFR-beta expression in the glomeruli was increased in transplant rejection and became detectable in tubules. Expression of NHERF-1 and NHERF-2 was not different in the patient groups. These results indicate that in contrast to the rat, both NHERF isoforms are detected in the human proximal tubule. In renal transplant rejection, there is increased expression of both PDGFR subtypes consistent with a role for PDGF in injury or repair.
authors.affiliation: Department of Medicine, University of Maryland School of Medicine, Baltimore, MD, USA. eweinman@medicine.umaryland.edu
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Weinman
authors.forename: Jay
authors.initials: J
authors.lastname: Lakkis
authors.forename: Michael
authors.initials: M
authors.lastname: Akom
authors.forename: Ravinder K
authors.initials: RK
authors.lastname: Wali
authors.forename: Cinthia B
authors.initials: CB
authors.lastname: Drachenberg
authors.forename: Richard A
authors.initials: RA
authors.lastname: Coleman
authors.forename: James B
authors.initials: JB
authors.lastname: Wade
mesh_terms: D000208:Acute Disease; D002908:Chronic Disease; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D006084:Graft Rejection / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D007678:Kidney Glomerulus / Q000378:metabolism / Q000473:pathology; D016030:Kidney Transplantation / Q000276:immunology / Q000473:pathology; D007687:Kidney Tubules, Proximal / Q000378:metabolism / Q000473:pathology; D010750:Phosphoproteins / Q000378:metabolism*; D017479:Receptors, Platelet-Derived Growth Factor / Q000378:metabolism*; D017923:Sodium-Hydrogen Exchangers
chemical_list: D003598:Cytoskeletal Proteins; D010750:Phosphoproteins; D017923:Sodium-Hydrogen Exchangers; C093376:sodium-hydrogen exchanger regulatory factor; D017479:Receptors, Platelet-Derived Growth Factor