title: Microbial transformation of ginsenoside Rb1 by Rhizopus stolonifer and Curvularia lunata.
abstract: Of 49 microbial strains screened for their capabilities to transform ginsenoside Rb1, Rhizopus stolonifer and Curvularia lunata produced four key metabolites: 3-O-[beta-D-glucopyranosyl-(1,2)-beta-D-glucopyranosyl]-20-O-[beta-D-glucopyranosyl]-3beta,12beta, 20(S)-trihydroxydammar-24-ene (1), 3-O-[beta-D-glucopyranosyl-(1,2)-beta-D-glucopyranosyl]-20-O-[beta-D-glucopyranosyl]-3beta,12beta, 20(S)-trihydroxydammar-24-ol (2), 3-O-[beta-D-glucopyranosyl-(1,2)-beta-D-glucopyranosyl]-3beta, 12beta, 20(S)-trihydroxydammar-24-ene (3), and 3-O-beta-D-glucopyranosyl-3beta, 12beta, 20(S)-trihydroxydammar-24-ene (4), identified by TOF-MS, 1H- and 13C-NMR spectral data. Metabolites 1, 3 and 4 were from the incubation with R. stolonifer, and 1 and 2 from the incubation with C. lunata. Compound 2 was identified as a new compound.
authors.affiliation: The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Xueyuan Road #38, Beijing 100083, PR China.
authors.forename: Aling
authors.initials: A
authors.lastname: Dong
authors.forename: Min
authors.initials: M
authors.lastname: Ye
authors.forename: Hongzhu
authors.initials: H
authors.lastname: Guo
authors.forename: Junhua
authors.initials: J
authors.lastname: Zheng
authors.forename: Dean
authors.initials: D
authors.lastname: Guo
mesh_terms: D001203:Ascomycota / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D001711:Biotransformation; D002855:Chromatography, Thin Layer / Q000379:methods; D036145:Ginsenosides / Q000737:chemistry* / Q000378:metabolism*; D012233:Rhizopus / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D036145:Ginsenosides; C442759:ginsenoside Rb1