title: Comparison of the gene expression profiles of monocytic versus granulocytic lineages of HL-60 leukemia cell differentiation by DNA microarray analysis.
abstract: It is now recognized that precise patterns of differentially expressed genes ultimately direct a particular cell toward a given lineage. In this study, we compared the expression profiles of cancer-related genes by cDNA microarray analysis during the differentiation of human promyelocytic leukemia HL-60 cells into either monocytes or granulocytes. RNA was isolated at times 0, 6, 12, 24, 36, 48, and 72 h following stimulation of differentiation with all-trans retinoic acid (all-trans RA) or 1,25-dihydroxyvitamin D(3) [1,25-(OH)(2)D(3)], and hybridized to the microarray gene chips containing 872 genes related to cell-cycles, oncogenes and leukemias. Several genes were commonly or differentially regulated during cell differentiation into either lineage, as demonstrated by both hierarchical and self-organizing map clustering analysis. At 72 h the expression levels of 45 genes were commonly up- or down-regulated at least a twofold in both lineages. Most importantly, 32 genes including alpha-L-fucosidase gene and adducin gamma subunit gene were up- or down-regulated only in all-trans RA-treated HL-60 cells, while 12 genes including interleukin 1beta and hypoxia-inducible factor 1alpha were up- or down-regulated only in 1,25-(OH)(2)D(3)-treated HL-60 cells. The expression of selected genes was confirmed by Northern blot analysis. As expected, some genes identified have not been examined during HL-60 cell differentiation into either lineage. The identification of genes associated with a specific differentiation lineage may give important insights into functional and phenotypic differences between two lineages of HL-60 cell differentiation.
authors.affiliation: College of Pharmacy and Research Institute of Drug Development, Chonnam National University, Kwangju, South Korea.
authors.forename: Ju Han
authors.initials: JH
authors.lastname: Song
authors.forename: Jong Man
authors.initials: JM
authors.lastname: Kim
authors.forename: Seung Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Hyeoung Joon
authors.initials: HJ
authors.lastname: Kim
authors.forename: Jae Jung
authors.initials: JJ
authors.lastname: Lee
authors.forename: Myung Hui
authors.initials: MH
authors.lastname: Sung
authors.forename: Seung Yong
authors.initials: SY
authors.lastname: Hwang
authors.forename: Tae Sung
authors.initials: TS
authors.lastname: Kim
mesh_terms: D002117:Calcitriol / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D019070:Cell Lineage / Q000235:genetics; D002470:Cell Survival / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis; D020869:Gene Expression Profiling; D015973:Gene Expression Regulation, Leukemic; D006098:Granulocytes / Q000473:pathology / Q000502:physiology*; D018922:HL-60 Cells / Q000502:physiology; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics* / Q000473:pathology; D009000:Monocytes / Q000473:pathology / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D012334:RNA, Neoplasm / Q000032:analysis; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D004273:DNA, Neoplasm; D012334:RNA, Neoplasm; D014212:Tretinoin; D002117:Calcitriol