Determination of seventeen polar/thermolabile pesticides in apples and apricots by liquid chromatography/mass spectrometry.
A simple liquid chromatography/mass spectrometry (LC/MS) approach for the determination of widely used representatives of polar/thermolabile pesticides in fruits was developed and validated. The group of pesticides comprised benzimidazoles and azoles (carbendazim, thiabendazole, imazalil, propiconazole, prochloraz, epoxiconazole, flusilazole, tebuconazole, bitertanol); N-methylcarbamates (carbaryl, carbofuran, methiocarb); and phenylureas and benzoylphenylureas (linuron, diflubenzuron, triflumuron, teflubenzuron, flufenoxuron). Matrixes (apple, apricot) were extracted with acetonitrile and crude extracts were cleaned up by solid-phase extraction (SPE) using either mixed cation exchange or hydrophilic lipophilic balance cartridges. LC separation of pesticides was performed on a reversed-phase column, Discovery C18. Electrospray ionization and ion trap MS/MS detection were applied. For most pesticides, overall recoveries ranged from 75 to 122%, and repeatability (as relative standard deviation) from 5 repetitive determinations of recovery ranged from 3 to 21%. Carbofuran was the only compound for which recovery was not satisfactory due to its loss in the SPE cleanup step. Limits of detection were 0.1-3 microg/kg for benzimidazole and azole fungicides and carbamate insecticides. For urea insecticides, detection limits were slightly higher (3-10 microg/kg).
Zrostlíková
Jitka
J
Institute of Chemical Technology, Technická 3, 166 28 Prague 6, Czech Republic.
Hajslová
Jana
J
Kovalczuk
Tomás
T
Stĕpán
Radim
R
Poustka
Jan
J
D002138:Calibration; D002853:Chromatography, Liquid; D027845:Malus / Q000737:chemistry*; D013058:Mass Spectrometry; D010573:Pesticide Residues / Q000032:analysis*; D027861:Prunus / Q000737:chemistry*; D015203:Reproducibility of Results
D010573:Pesticide Residues