title: Effect of polyphenolic compounds on the renal Na(+),K(+)-ATPase during the restoration of normotension after experimentally induced hypertension in rats.
abstract: It is commonly known that consumption of foods and beverages rich in polyphenols is associated with a lower incidence of cardiovascular disease. The purpose of this study was to assess whether the application of red wine polyphenols influences the kinetic properties of renal Na(+),K(+)-ATPase in rats in which hypertension has been experimentally induced by the nitric oxide synthase inhibitor L-NAME. Treatment with polyphenols during the recovery from hypertension to normotension resulted in the complete revival of the functional properties of the Na(+),K(+)-ATPase, as indicated by the total restoration of K(m), K(Na) (concentration of Na(+) necessary to achieve half-maximal reaction velocity) and V(max) for enzyme activation by ATP and/or Na(+) to pre-hypertension values. Two positive effects of polyphenols during the recovery period are indicated: a restoration of the affinity of the ATP and Na(+) binding sites to control values and a probable increase in the number of Na(+),K(+)-ATPase molecules to a level comparable to that in control conditions, as suggested by the complete renewal of V(max).
authors.affiliation: Institute for Heart Research, Department of Biochemistry, Slovak Academy of Sciences, PO Box 104, 840 05 Bratislava 45, Slovak Republic.
authors.forename: Veronika
authors.initials: V
authors.lastname: Javorková
authors.forename: Olga
authors.initials: O
authors.lastname: Pechánová
authors.forename: Ramaroson
authors.initials: R
authors.lastname: Andriantsitohaina
authors.forename: Norbert
authors.initials: N
authors.lastname: Vrbjar
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D004791:Enzyme Inhibitors; D005419:Flavonoids / Q000494:pharmacology*; D006973:Hypertension / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D008297:Male; D019331:NG-Nitroarginine Methyl Ester; D019001:Nitric Oxide Synthase / Q000378:metabolism; D010636:Phenols / Q000494:pharmacology*; D059808:Polyphenols; D051381:Rats; D011921:Rats, Inbred WKY; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
chemical_list: D004791:Enzyme Inhibitors; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D000255:Adenosine Triphosphate; D019001:Nitric Oxide Synthase; D000254:Sodium-Potassium-Exchanging ATPase; D019331:NG-Nitroarginine Methyl Ester