title: Metabolic partitioning of endogenous fatty acid in adipocytes.
abstract: OBJECTIVE
  To develop an accurate new method to measure the partitioning of adipocyte endogenous fatty acids among different metabolic pathways, a critical step toward understanding the regulatory mechanism by which fat disposition is modulated.
  
  
  METHODS
  Isolated primary rat adipocytes were pre-incubated with isotope-labeled fatty acids. This allows determination of the specific activity of labeled fatty acids in the endogenous lipid pool. After the removal of exogenous fatty acids, the disposition of endogenous fatty acids into the three major metabolic pathways, namely, oxidation, re-esterification, and release into the medium, was measured independently. This was compared with the total lipolytic release of endogenous fatty acids, as measured by glycerol release. Adipocytes from normal fed and fasted animals were used to determine the effects of physiological variations on the metabolic fate of endogenous fatty acids.
  
  
  RESULTS
  In normal fed animals, 0.2% of endogenous fatty acids were oxidized, 50.1% were released, and 49.7% were re-esterified. Fasting doubled the partitioning of fatty acids toward oxidation (p < 0.05) in association with increased lipolysis (1.4-fold increase) (p < 0.05). This effect was completely abolished by the addition of insulin to the cells (61% reduction) (p < 0.05).
  
  
  CONCLUSIONS
  The endogenous fatty acids in adipocytes are actively oxidized. This process can be regulated by altered physiological conditions or by insulin. Over time, it is possible that a small shift of fatty acids toward oxidation could have a significant impact on body fuel economy. This hypothesis needs to be tested.
authors.affiliation: Obesity Research Center, Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118, USA.
authors.forename: Tong
authors.initials: T
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Zang
authors.forename: Wenhua
authors.initials: W
authors.lastname: Ling
authors.forename: Barbara E
authors.initials: BE
authors.lastname: Corkey
authors.forename: Wen
authors.initials: W
authors.lastname: Guo
mesh_terms: D017667:Adipocytes / Q000737:chemistry* / Q000378:metabolism*; D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D002250:Carbon Radioisotopes; D004951:Esterification; D005215:Fasting; D005227:Fatty Acids / Q000032:analysis* / Q000378:metabolism*; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D005502:Food; D005990:Glycerol / Q000378:metabolism; D008066:Lipolysis; D010084:Oxidation-Reduction; D051381:Rats; D014280:Triglycerides / Q000378:metabolism; D014316:Tritium; D014867:Water / Q000378:metabolism
chemical_list: D002250:Carbon Radioisotopes; D005227:Fatty Acids; D005230:Fatty Acids, Nonesterified; D014280:Triglycerides; D014867:Water; D014316:Tritium; D002245:Carbon Dioxide; D005990:Glycerol