Intracellular expression of the Salmonella plasmid virulence protein, SpvB, causes apoptotic cell death in eukaryotic cells.
The spv genes carried on the Salmonella virulence plasmid are commonly associated with severe systemic infection in experimental animals. The SpvB virulence-associated protein has been shown to ADP-ribosylate actin, and this enzymatic activity is essential for virulence in mice. Here, we present evidence that intracellular expression of SpvB protein induces not only disruption of actin filaments but also apoptotic cell death in eukaryotic cells.
Kurita
Ai
A
Laboratory of Molecular Immunology, Center for Basic Research, The Kitasato Institute, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8642, Japan.
Gotoh
Hideo
H
Eguchi
Masahiro
M
Okada
Nobuhiko
N
Matsuura
Satoshi
S
Matsui
Hidenori
H
Danbara
Hirofumi
H
Kikuchi
Yuji
Y
D016475:3T3 Cells; D036002:ADP Ribose Transferases / Q000096:biosynthesis* / Q000378:metabolism / Q000502:physiology; D008841:Actin Cytoskeleton / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D019556:COS Cells; D002522:Chlorocebus aethiops; D020287:In Situ Nick-End Labeling; D051379:Mice; D012475:Salmonella / Q000378:metabolism* / Q000472:pathogenicity; D012480:Salmonella Infections; D014774:Virulence; D037521:Virulence Factors / Q000096:biosynthesis* / Q000502:physiology
D037521:Virulence Factors; D036002:ADP Ribose Transferases; C415223:spvB protein, Salmonella enterica virulence plasmid