E4orf3 is necessary for enhanced S-phase replication of cell cycle-restricted subgroup C adenoviruses.
E1B-55K-mutant or E4orf6-mutant adenoviruses replicate more effectively after infecting cells in S phase than after infecting cells in G(1). Enhanced S-phase replication of the E4orf6-mutant viruses requires the E4orf3 gene. This report demonstrates that the E4orf3 gene is also required for enhanced S-phase replication of the E1B-55K-mutant virus.
Shepard
Robin N
RN
Department of Microbiology and Immunology, Wake Forest University School of Medicine, Wake Forest University, Winston-Salem, North Carolina 27157-1064, USA.
Ornelles
David A
DA
D017501:Adenovirus E1B Proteins / Q000235:genetics / Q000378:metabolism; D017504:Adenovirus E4 Proteins / Q000235:genetics / Q000378:metabolism*; D000260:Adenoviruses, Human / Q000145:classification / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D002453:Cell Cycle; D016193:G1 Phase; D006367:HeLa Cells; D006801:Humans; D009154:Mutation; D016196:S Phase; D014779:Virus Replication
D017501:Adenovirus E1B Proteins; D017504:Adenovirus E4 Proteins