Flow injection-capillary electrophoresis system with contactless conductivity detection and hydrostatic pressure generated flow. Application to the quantitative analysis of inorganic anions in water samples.
A simple and inexpensive flow injection-capillary electrophoresis (FI-CE) system with contactless conductivity detection (CCD) for automated quantitative analysis of chloride, nitrate, and sulfate in various water samples is demonstrated. A glass bottle containing the background electrolyte that is raised above the FI-CE interface generates a pulse-free, highly reproducible flow of the electrolyte through the FI-CE interface. The system operates at a flow rate of 300 microLmin(-1) with an injection volume of only 4 microL. The repeatability of peak areas (n = 18) was better than 0.81% RSD and the sample throughput was 90 samples per hour using the background electrolyte containing 12 mM L-histidine adjusted to pH 4.00 with acetic acid. The limits of detection were better than 125 microgL(-1) and were comparable to those obtained by conventional CE systems with CCD. Various calibration methods for FI-CE system with electrokinetic injection were tested and their suitability for the analysis of anions in real samples was evaluated.
Kubáň
Pavel
P
Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Brno, Czech Republic.
Kubáň
Petr
P
Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Brno, Czech Republic.|Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA.
Kubáň
Vlastimil
V
Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Brno, Czech Republic.
D000838:Anions; D002138:Calibration; D019075:Electrophoresis, Capillary / Q000379:methods*; D017022:Flow Injection Analysis / Q000379:methods*; D007287:Inorganic Chemicals / Q000302:isolation & purification*; D011312:Pressure; D015203:Reproducibility of Results; D014867:Water / Q000737:chemistry*
D000838:Anions; D007287:Inorganic Chemicals; D014867:Water