[Study on the expressions of basic fibroblast growth factor and nervous growth factor genes in rat cerebral concussion].
OBJECTIVE
To study the expressions of basic fibroblast growth factor (bFGF) and nervous growth factor(NGF) genes in rat cerebral concussion.


METHODS
Eighty Wistar male rats were used for animal model of cerebral concussion, which were sacrificed on 1, 3, 7, 14 and 30 days after injury and the brain tissue was taken out. The expressions of bFGF and NGF genes were studied in the course of cerebral concussion by means of immunohistochemistry and in situ hybridization.


RESULTS
Rats in 100 g group were seen the clinical manifestation for typical cerebral concussion. The protein and mRNA of bFGF were increased on day 1, obtained at peak on day 3-7, decreased on day 14 and also increased on day 30 compared with controls. The positive area was seen in the plasma of neurons in cerebral cortex, hippocampus, thalamus and cerebellum. NGF protein and mRNA showed strong positive and increased in the plasma of neurons in cerebral cortex, hippocampus, thalamus and cerebellum on day 1, and they were continuously positive but gradually decreased within 30 days after injury.


CONCLUSIONS
The expression of bFGF gene participates in the course of cerebral concussion, might play an important role in the nervous cells degeneration and necrosis; NGF gene expression participates in the whole course of cerebral concussion, especially in the early phase.
Peng
Rui-yun
RY
Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
Gao
Ya-bing
YB
Xiao
Xing-yi
XY
Wang
De-wen
DW
Chen
Hao-yu
HY
Wu
Xiao-hong
XH
Liu
Jie
J
Hu
Wen-hua
WH
Cai
Bao-ren
BR
D000818:Animals; D001921:Brain / Q000378:metabolism; D001924:Brain Concussion / Q000235:genetics / Q000378:metabolism*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D017403:In Situ Hybridization; D008297:Male; D009414:Nerve Growth Factors / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D009414:Nerve Growth Factors; D016222:Fibroblast Growth Factor 2