title: [The inhibition effect of photodynamic on human Tenon capsule fibroblast cells].
abstract: OBJECTIVE
  To investigate the inhibition of photodynamic effect on human Tenon capsule fibroblast cells in vitro.
  
  
  METHODS
  The human Tenon capsule fibroblast cells were divided into nine groups named as group A to I, each group had four wells. Group A to G, each group was added photosensitizer benzoporphyrin derivative monoacid ring A (BPD) with end concentration of 2.50 x 10(3) g/L, 1.25 x 10(3) g/L, 0.62 x 10(3) g/L, 0.31 x 10(3) g/L, 0.16 x 10(3) g/L, 0.08 x 10(3) g/L, 0.04 x 10(3) g/L respectively. Then the culture cells were irradiated by 689 nm diode laser with dosage of 2.4 J/cm(2) after BPD treatment for 15 minutes. Group H was treated with Mitomycin C (MMC) at concentration of 0.2 g/L. Group I was control without any treatment. All the cells were kept cultured for another 24 hours and then MTT colorimetric assay was applied to measure the relative inhibitory rate of photodynamic effect on the cells.
  
  
  RESULTS
  There is statistical significance between group A to H and group I with the method of one way analysis of variance. When compared to group I, the relative inhibitory rates of group A to G are 93.3%, 91.0%, 90.3%, 87.1%, 66.0%, 41.6%, 12.5% respectively, and the inhibitory rate of group H (MMC) is 93.0%.
  
  
  CONCLUSIONS
  Photodynamic effect can inhibit the proliferation of human Tenon capsule fibroblast cells in vitro. The inhibitory rate appears to be dependent on the concentration of the photo sensitizer.
authors.affiliation: Zhongshan Ophthalmic Center, Zhongshan University, Guangzhou 510060, China. hy-c@163.net
authors.forename: Hui-yi
authors.initials: HY
authors.lastname: Chen
authors.forename: Jian
authors.initials: J
authors.lastname: Ge
authors.forename: Yan
authors.initials: Y
authors.lastname: Guo
authors.forename: Chen-jin
authors.initials: CJ
authors.lastname: Jin
authors.forename: Yu-qing
authors.initials: YQ
authors.lastname: Lan
authors.forename: Ming-kai
authors.initials: MK
authors.lastname: Lin
mesh_terms: D002455:Cell Division / Q000187:drug effects; D003228:Conjunctiva / Q000166:cytology* / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000008:administration & dosage / Q000494:pharmacology*; D011166:Porphyrins / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D017319:Photosensitizing Agents; D011166:Porphyrins