title: Glucocorticoid modulation of extracellular signal-regulated protein kinase 1/2 and p38 in human ovarian cancer HO-8910 cells.
abstract: OBJECTIVE
  To investigate the signaling pathway through testing the effects of dexamethasone (Dex) on the activation of the extracellular signal-regulated protein kinase 1/2 (ERK1/2) and p38 kinase (p38) in HO-8910 cells.
  
  
  METHODS
  Activation of the ERK1/2 and p38 was detected by Western blotting using the antibodies against the total ERK1/2 and p38 mitogen-activated protein kinases (MAPKs) protein and the phosphorylated forms of them.
  
  
  RESULTS
  Dex could suppress the activation of ERK1/2, while enhance the activation of p38 rapidly and strongly in a dose- and time- dependent manner. Neither effect could be blocked by RU486, the antagonist of glucocorticoid receptor (GR).
  
  
  CONCLUSIONS
  Dex has rapid effects on the activation of ERK1/2 and p38, and these effects are not mediated by GR.
authors.affiliation: Department of Pathophysiology, Second Military Medical University, Shanghai 200433, China. xiabing72@hotmail.com
authors.forename: Bing
authors.initials: B
authors.lastname: Xia
authors.forename: Jian
authors.initials: J
authors.lastname: Lu
authors.forename: Gang
authors.initials: G
authors.lastname: Wang
mesh_terms: D002455:Cell Division / Q000502:physiology; D003907:Dexamethasone / Q000494:pharmacology*; D004789:Enzyme Activation; D005260:Female; D005938:Glucocorticoids / Q000494:pharmacology*; D006801:Humans; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D010051:Ovarian Neoplasms / Q000201:enzymology / Q000473:pathology; D015398:Signal Transduction; D014407:Tumor Cells, Cultured; D048051:p38 Mitogen-Activated Protein Kinases
chemical_list: D005938:Glucocorticoids; D003907:Dexamethasone; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases