title: Phase variation in the phage growth limitation system of Streptomyces coelicolor A3(2).
abstract: The phase-variable phage growth limitation (Pgl) system of Streptomyces coelicolor A3(2) is an unusual bacteriophage resistance mechanism that confers protection against the temperate phage phiC31 and homoimmune relatives. Pgl is subject to phase variation, and data presented here show that this is at least partially due to expansion and contraction of a polyguanine tract present within the putative adenine-specific DNA methyltransferase gene, pglX. Furthermore, the pglX paralogue SC6G9.02, here renamed pglS, was shown to be able to interfere with the Pgl phenotype, suggesting that PglS could provide an alternative activity to that conferred by PglX.
authors.affiliation: Institute of Genetics, University of Nottingham, Queens Medical Centre, Nottingham, NG7 2UH, United Kingdom.
authors.forename: Paul
authors.initials: P
authors.lastname: Sumby
authors.forename: Margaret C M
authors.initials: MC
authors.lastname: Smith
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D001435:Bacteriophages / Q000502:physiology*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); D013302:Streptomyces / Q000235:genetics / Q000254:growth & development / Q000821:virology*; D010948:Viral Plaque Assay
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; C095144:PglY protein, Streptomyces coelicolor; C095145:PglZ protein, Streptomyces coelicolor; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)