title: Screen-printed multienzyme arrays for use in amperometric batch and flow systems.
abstract: Screen-printing technology for electrode fabrication enables construction of amperometric devices suitable for combination of several enzyme electrodes. To develop a biosensor array for characterisation of wastewaters, tyrosinase and horseradish peroxidase (HRP) or cholinesterase-modified electrodes were combined on the same array. The behaviour of the tyrosinase-modified electrode in the presence of hydrogen peroxide (required co-substrate for the HRP-modified electrode) and acetylthiocholine chloride (required co-substrate for cholinesterase) was studied. Performance of bi-enzyme biosensor arrays in the batch mode and in the flow-injection system are discussed.
authors.affiliation: Department of Analytical Chemistry, Lund University, P.O. Box 124, 22 100 Lund, Sweden. Svetlana.Sapelnikova@analykem.lu.se
authors.forename: S
authors.initials: S
authors.lastname: Sapelnikova
authors.forename: E
authors.initials: E
authors.lastname: Dock
authors.forename: R
authors.initials: R
authors.lastname: Solná
authors.forename: P
authors.initials: P
authors.lastname: Skládal
authors.forename: T
authors.initials: T
authors.lastname: Ruzgas
authors.forename: J
authors.initials: J
authors.lastname: Emnéus
mesh_terms: D000110:Acetylcholinesterase; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002396:Catechols / Q000032:analysis; D002802:Cholinesterases; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D004800:Enzymes, Immobilized; D017022:Flow Injection Analysis; D006735:Horseradish Peroxidase; D006861:Hydrogen Peroxide; D014442:Monophenol Monooxygenase; D009097:Multienzyme Complexes / Q000737:chemistry*; D010636:Phenols / Q000032:analysis; D015203:Reproducibility of Results; D014874:Water Pollutants, Chemical / Q000032:analysis
chemical_list: D002396:Catechols; D004800:Enzymes, Immobilized; D009097:Multienzyme Complexes; D010636:Phenols; D014874:Water Pollutants, Chemical; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D014442:Monophenol Monooxygenase; D000110:Acetylcholinesterase; D002802:Cholinesterases