title: Rev binds specifically to a purine loop in the SL1 region of the HIV-1 leader RNA.
abstract: The leader RNA sequence of human immunodeficiency virus type 1 (HIV-1) consists of a complex series of stem loop structures that are critical for viral replication. Three-dimensional structural analysis by NMR of one of these structures, the SL1 stem loop of the packaging signal region, revealed a highly conserved purine rich loop with a structure nearly identical to the Rev-binding loop of the Rev response element. Using band-shift assays, surface plasmon resonance, and further NMR analysis, we demonstrate that this loop binds Rev. HIV-1 appears to have a second Rev-binding site close to the major splice donor site that may have an additional role in the viral life cycle.
authors.affiliation: Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom.
authors.forename: Jose
authors.initials: J
authors.lastname: Gallego
authors.forename: Jane
authors.initials: J
authors.lastname: Greatorex
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
authors.forename: Shyamala
authors.initials: S
authors.lastname: Arunachalam
authors.forename: Jianhua
authors.initials: J
authors.lastname: Fang
authors.forename: John
authors.initials: J
authors.lastname: Seamons
authors.forename: Susan
authors.initials: S
authors.lastname: Lea
authors.forename: Roger J
authors.initials: RJ
authors.lastname: Pomerantz
authors.forename: Andrew M L
authors.initials: AM
authors.lastname: Lever
mesh_terms: D020121:5' Untranslated Regions / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D015694:Gene Products, rev / Q000235:genetics / Q000378:metabolism*; D015752:Genes, env; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism*; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D016296:Mutagenesis; D019906:Nuclear Magnetic Resonance, Biomolecular; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017384:Sequence Deletion; D020349:Surface Plasmon Resonance; D054321:rev Gene Products, Human Immunodeficiency Virus
chemical_list: D020121:5' Untranslated Regions; D015694:Gene Products, rev; D012367:RNA, Viral; D054321:rev Gene Products, Human Immunodeficiency Virus