[The potential role of high mobility group-1 protein in the pathogenesis of sepsis-induced multiple organ dysfunction syndrome in rats].
OBJECTIVE
To investigate the potential role of high mobility group-1 protein (HMG-1) in the pathogenesis of sepsis-induced multiple organ dysfunction syndrome in rats.


METHODS
Using a sepsis model by cecal ligation and puncture (CLP), 80 male Wistar rats were randomly divided into four groups: normal control (n = 10), sham operation (n = 10), CLP (subdivided into 2, 6, 12, 24, 48, 72 h post-CLP, n = 60), and sodium butyrate treatment (subdivided into 12, 24 h post-CLP, n = 20). At serial time points in each group, animals were sacrificed, and blood as well as tissue samples from the liver, lung, kidney and small intestine were harvested to measure organ function parameters and HMG-1 mRNA expression by the reverse transcription polymerase chain reaction (RT-PCR) taking GAPDH as an internal standard. Also, additional experiments were performed to observe the effect of treatment with sodium butyrate on survival rate in septic rats (n = 57).


RESULTS
HMG-1 mRNA levels significantly increased in various tissues during 6 - 72 h after CLP (P < 0.05 or 0.01), and were markedly inhibited by sodium butyrate at 12 h and 24 h (P < 0.05 or 0.01). Early treatment with sodium butyrate also could markedly reduce serum alanine aminotransferase, creatinine levels at 12 h post-CLP and pulmonary myeloperoxidase activities at 24 h. Furthermore, treatment with sodium butyrate could significantly improve the 1- to 6-day survival rates in animals subjected to CLP (P < 0.05 or 0.01).


CONCLUSIONS
HMG-1 might play an important role in the development of excessive inflammatory response and subsequent multiple organ dysfunction syndrome.
Zhang
Li-tian
LT
Trauma Research Center, 304th Hospital of People's Liberation Army, Beijing 100037, China.
Yao
Yong-ming
YM
Lu
Jia-qi
JQ
Dong
Ning
N
Yu
Yan
Y
Yan
Xiao-jian
XJ
Fang
Wen-hui
WH
Sheng
Zhi-yong
ZY
D000818:Animals; D004195:Disease Models, Animal; D024243:HMGB1 Protein / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D008297:Male; D009102:Multiple Organ Failure / Q000209:etiology* / Q000503:physiopathology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018805:Sepsis / Q000150:complications*; D016019:Survival Analysis
D024243:HMGB1 Protein; D012333:RNA, Messenger