Expression of local renin and angiotensinogen mRNA in cirrhotic portal hypertensive patient.
OBJECTIVE
To investigate the expression of local renin and angiotensinogen mRNA in cirrhotic portal hypertensive patients.


METHODS
The expression of local renin and angiotensinogen mRNA in the liver, splenic artery and vein of PH patients was detected by RT-PCR analysis.


RESULTS
Expression of local renin mRNA in the liver of control group was (0.19+/-0.11), significantly lower than that in splenic artery (0.45+/-0.17) or splenic vein(0.39+/-0.12) respectively, (P<0.05). Expression of local angiotensinogen mRNA in the liver was (0.64+/-0.21), significantly higher than that in splenic artery(0.41+/-0.15) or in splenic vein (0.35+/-0.18) respectively, (P<0.05). Expression of local renin mRNA in the liver, splenic artery and vein of PH group was (0.78+/-0.28), (0.86+/-0.35) and (0.81+/-0.22) respectively, significantly higher than that in the control group, (P<0.05). Expression of local angiotensinogen mRNA in the liver, splenic artery and vein of PH group was (0.96+/-0.25), (0.83+/-0.18) and (0.79+/-0.23) respectively, significantly higher than that in the control group, (P<0.05). There was no significant difference between the liver, splenic artery and vein in the expression of local renin or local angiotensinogen mRNA in PH group, (P<0.05).


CONCLUSIONS
In normal subjects the expression of local renin and angiotensinogen mRNA was organ specific, but with increase of the expression of LRAS, the organ-specificity became lost in cirrhotic patients. LRAS may contribute to increased resistance of portal vein with liver and formation of splanchnic vasculopathy.
Zhang
Li
L
Department of General Surgery, Shandong Provincial Hospital, Jinan 250021, Shandong Province, China. pzzl@sina.com
Yang
Zhen
Z
Shi
Bao-Min
BM
Li
Da-Peng
DP
Fang
Chong-Yun
CY
Qiu
Fa-Zu
FZ
D000328:Adult; D000368:Aged; D000808:Angiotensinogen / Q000235:genetics*; D004730:Endothelium, Vascular / Q000473:pathology / Q000648:ultrastructure; D005260:Female; D015870:Gene Expression; D006801:Humans; D006975:Hypertension, Portal / Q000473:pathology / Q000503:physiopathology*; D007150:Immunohistochemistry; D008103:Liver Cirrhosis / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D009928:Organ Specificity; D012333:RNA, Messenger / Q000032:analysis; D012083:Renin / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013162:Splenic Vein / Q000473:pathology
D012333:RNA, Messenger; D000808:Angiotensinogen; D012083:Renin