title: An amino acid substitution on the second acetylcholinesterase in the pirimicarb-resistant strains of the peach potato aphid, Myzus persicae.
abstract: cDNAs encoding two acetylcholinesterases (AChEs) were isolated from the peach potato aphid, Myzus persicae. MpAChE1 was orthologous and MpAChE2 was paralogous with the ace of Drosophila melanogaster. The deduced amino acid sequence of MpAChE1 cDNA was identical between the pirimicarb susceptible and resistant strains. However, a single amino acid substitution of Ser431Phe on MpAchE2 was found in the pirimicarb resistant strains. This substitution was located in the acyl pocket of the enzyme and was thought to alter the ligand specificity.
authors.affiliation: Laboratory of Applied Zoology E407, Department of Agriculture and Forestry, Institute of Agriculture and Forestry, University of Tsukuba, Tennodai 1-1-1, Ibaraki, 305-8572, Japan. mtmikami@sakura.cc.tsukuba.ac.jp
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nabeshima
authors.forename: Toshinori
authors.initials: T
authors.lastname: Kozaki
authors.forename: Takashi
authors.initials: T
authors.lastname: Tomita
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kono
mesh_terms: D000110:Acetylcholinesterase / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D001042:Aphids / Q000187:drug effects / Q000201:enzymology*; D002219:Carbamates / Q000494:pharmacology*; D020134:Catalytic Domain; D018076:DNA, Complementary / Q000235:genetics; D007305:Insecticide Resistance; D007306:Insecticides / Q000494:pharmacology*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D011487:Protein Conformation; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D011743:Pyrimidines
chemical_list: D002219:Carbamates; D018076:DNA, Complementary; D007306:Insecticides; D020033:Protein Isoforms; D011743:Pyrimidines; C011994:pirimicarb; D000110:Acetylcholinesterase