Analysis of the T cell receptor V beta repertoire in 2,4,6-trinitrobenzenesulfonic acid induced colitis in mice.
The aim of this study was to analyze which types of T cells are at work and the specific nature of their response, using a mouse 2,4,6-trinitrobenzenesulfonic acid (TNBS) induced colitis model. The response of T cells to TNBS was analyzed by anti-TNBS mixed-lymphocyte reaction. T cell clones were established by limiting dilution. Phenotypes and T cell receptor (TCR) V beta of T cells were analyzed by flow cytometry. Colitis was induced by administration of TNBS enemas, and lamina propria lymphocytes were isolated and analyzed. The proliferative responses to TNBS of spleen T cells were partially inhibited by the addition of antimouse CD4 or CD8 antibodies to the mixed-lymphocyte culture. Conversely, these were inhibited by the addition of both antibodies. Flow cytometric analysis showed that TCR V beta 14 T cells specifically increased in the CD8+ T cell population. We established CD8+ TCR V beta 14 T cell clones which were TNBS reactive and self-restricted. Investigation using lamina propria lymphocytes in TNBS-induced colitis revealed that the rate of CD8+ TCR V beta 14 T cells changed with histological inflammatory activity which also attained a peak on day 5 following enema administration. Both CD4+ and CD8+ T cell subsets responded to TNBS, and the rate of CD8+ TCR V beta 14 T cells changed with histological inflammatory activity in TNBS-induced colitis.
Kanetake
Kazuto
K
1st Department of Surgery, Gifu University School of Medicine, Gifu, Japan.
Iwata
Hisashi
H
Mori
Yoshio
Y
Takagi
Hisato
H
Hirota
Toshio
T
Nitta
Toyoo
T
Hayashi
Masatomo
M
Onitsuka
Atsuyoshi
A
Hirose
Hajime
H
D000818:Animals; D003092:Colitis / Q000139:chemically induced / Q000276:immunology*; D005260:Female; D007202:Indicators and Reagents / Q000009:adverse effects*; D051379:Mice; D023421:Models, Animal; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D014302:Trinitrobenzenesulfonic Acid / Q000009:adverse effects*
D007202:Indicators and Reagents; D016693:Receptors, Antigen, T-Cell, alpha-beta; D014302:Trinitrobenzenesulfonic Acid