Pharmacological characterization of micropatterned cardiac myocytes.
The cardiac myocytes patterned on a single cell level were prepared by microcontact printing, and the response to chemical stimuli was studied using confocal fluorescent Ca(2+) imaging. The patterned myocytes were found to conjugate by forming gap junction. It was confirmed for the patterned myocytes that gap junction communication was reversibly inhibited by 1-octanol, but activated by caffeine. Localized stimulation with chemicals was also attempted using a microinjection system for the myocyte patterns formed by single cell alignment. This research was carried out with the objective of developing a bioassay system based on a cellular network.
Kaji
Hirokazu
H
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Sendai, 980-8579, Japan.
Takii
Yukii
Y
Nishizawa
Matsuhiko
M
Matsue
Tomokazu
T
D020003:1-Octanol / Q000494:pharmacology; D001672:Biocompatible Materials; D001681:Biological Assay / Q000379:methods; D002110:Caffeine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002448:Cell Adhesion; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D005353:Fibronectins / Q000737:chemistry; D017629:Gap Junctions; D006801:Humans; D018613:Microscopy, Confocal; D008858:Microscopy, Phase-Contrast; D009206:Myocardium / Q000166:cytology*; D012828:Silicones / Q000737:chemistry; D013997:Time Factors
D001672:Biocompatible Materials; D004129:Dimethylpolysiloxanes; D005353:Fibronectins; D012828:Silicones; D002110:Caffeine; C013830:baysilon; D020003:1-Octanol; D002118:Calcium