Synthesis, characterization, and use of 2-[(2H(9))butoxy]acetic acid and 2-(3-methylbutoxy)acetic acid as an internal standard and an instrument performance surrogate, respectively, for the gas chromatographic-mass spectrometric determination of 2-butoxyacetic acid, a human metabolite of 2-butoxyethanol.
2-[(2H(9))Butoxy]acetic acid and 2-(3-methylbutoxy)acetic acid were synthesized, mixed with 2-butoxyacetic acid, and separated by capillary gas chromatography on a fused-silica column with a length of 50 m, inside diameter of 0.200 mm, and a "free fatty acid phase" wall coating of 0.3 microm film. 2-[(2H(9))Butoxy]acetic acid, 2-butoxyacetic acid, and 2-(3-methylbutoxy)acetic acid were baseline resolved at retention times of 13.55, 13.78, and 15.20 min; 2-(3-methylbutoxy)acetic acid having a peak efficiency of 360,000. Mass spectrometric detection using selected ion monitoring at m/z 66, 57, and 71 showed linear analytical responses from 0.04 ng to at least 200 ng with a limit of detection of 0.04 ng for 2-butoxyacetic acid.
Brown
Kenneth K
KK
National Institute for Occupational Safety and Health, 4676 Columbia Parkway, R-7 Cincinnati, OH 45226, USA. krb1@cdc.gov
Cheever
Kenneth L
KL
Butler
Mary Ann
MA
Shaw
Peter B
PB
McLaurin
Jeffery L
JL
D002138:Calibration; D005026:Ethylene Glycols / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006016:Glycolates / Q000032:analysis* / Q000737:chemistry*; D006801:Humans; D012015:Reference Standards; D012680:Sensitivity and Specificity
C482879:2-(3-methylbutoxy)acetic acid; D005026:Ethylene Glycols; D006016:Glycolates; C017095:n-butoxyacetic acid; C017096:n-butoxyethanol