Effects on telomerase activity and associated-protein of hRPE cells by TGF-beta 1.
OBJECTIVE
To investigate the effects on telomerase activity, human telomerase reverse transcriptase(hTERT) gene and TEP1mRNA of retinal pigment epithelial(RPE) cells were treated by TGF-beta 1 of different concentration.


METHODS
The cultured human RPE cells were treated with TGF-beta 1 at different concentration (0 ng/ml, 0.01 ng/ml, 0.1 ng/ml, 1 ng/ml, 10 ng/ml) for 24 h, then telomerase activity was detected by telomerase repeat amplification protocol (TRAP). The expression of hTERT mRNA and TEP-1mRNA were detected by reverse transcription polymerase chain reaction (RT-PCR).


RESULTS
TRAP and RT-PCR showed when the concentration of TGF-beta 1 was gradually increased, telomerase activity and the expression of hTERTmRNA were gradually reduced, TEP1mRNA showed no apparent differential expression.


CONCLUSIONS
TGF-beta 1 can down-regulate telomerase activity and the expression of hTERT mRNA, but no effection on TEP-1mRNA, hTERTmRNA expression was in accordance with telomerase activity in hRPE cells, hTERT gene plays a crucial role in the expression of telomerase activity, while TEP1 plays a much smaller role.
Zhao
Hong
H
Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Zeng
Shuiqing
S
Zhu
Xiaohong
X
Zuo
Zhigao
Z
Zeng
Qingguang
Q
Chao
Weijing
W
D002455:Cell Division; D002478:Cells, Cultured; D004268:DNA-Binding Proteins; D006801:Humans; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000201:enzymology*; D012313:RNA / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019098:Telomerase / Q000235:genetics / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1
D004268:DNA-Binding Proteins; D012333:RNA, Messenger; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; C422952:telomerase RNA; D012313:RNA; D019098:Telomerase