Logistics and quality control for DNA sampling in large multicenter studies.
To study associations between genetic variation and disease, large bio-banks need to be created in multicenter studies. Therefore, we studied the effects of storage time and temperature on DNA quality and quantity in a simulation experiment with storage up to 28 days frozen, at 4 degrees C and at room temperature. In the simulation experiment, the conditions did not influence the amount or quality of DNA to an unsatisfactory level. However, the amount of extracted DNA was decreased in frozen samples and in samples that were stored for > 7 days at room temperature. In a sample of patients from 24 countries of the EUROPA trial obtained by mail with transport times up to 1 month DNA yield and quality were adequate. From these results we conclude that transport of non-frozen blood by ordinary mail is usable and practical for DNA isolation for polymerase chain reaction in clinical and epidemiological studies.
Nederhand
R J
RJ
Gaubius Laboratory, TNO Prevention and Health, Leiden, the Netherlands.
Droog
S
S
Kluft
C
C
Simoons
M L
ML
de Maat
M P M
MP
D001771:Blood Banks / Q000592:standards; D001773:Blood Cells; D001800:Blood Specimen Collection / Q000379:methods / Q000592:standards; D004247:DNA / Q000302:isolation & purification*; D006801:Humans; D009934:Organization and Administration; D011309:Preservation, Biological / Q000379:methods* / Q000592:standards; D011786:Quality Control; D013696:Temperature; D013997:Time Factors; D014186:Transportation / Q000592:standards; D000094902:Blood Banking / Q000379:methods
D004247:DNA