title: [Effect of negative pressure suction on rabbit optic nerve and retina].
abstract: OBJECTIVE
  To study the effect of acute intraocular pressure (IOP) elevation on the optic nerve and retina in laser in situ keratomileusis (LASIK).
  
  
  METHODS
  Acute IOP was increased to 65 mm Hg for different period of time (30 s, 1 min and 3 min) by using scleral suction on rabbit eyes. The tissues of retina and optic nerve of immediately extirpated group (instant group), of 2 weeks later extirpated group (recovery group) both after the negative pressure suction and of normal rabbit eyes were examined with electron and light microscope.
  
  
  RESULTS
  After the negative pressure suction for 30 s, 1 min, and 3 min respectively, the optic nerve and retinal cells changed lightly; some part of optic nerve and retinal cells changed; optic nerve fibers and retinal cells changed sharply.
  
  
  CONCLUSIONS
  Ultrastructural changes of retina and optic nerve may be induced by acute IOP elevation in LASIK. The longer the time, the more apparent the changes.
authors.affiliation: Eye Research Center, Chinese Academy of Medical Sciences & Chinese Union Medical College, Department of Ophthalmology, Peking Union Medical College Hospital, Beijing 100730, China. awai@263.net
authors.forename: A-Li
authors.initials: AL
authors.lastname: Ye
authors.forename: Guo-xiang
authors.initials: GX
authors.lastname: Pang
authors.forename: Ye-hua
authors.initials: YH
authors.lastname: Han
mesh_terms: D000818:Animals; D005260:Female; D020731:Keratomileusis, Laser In Situ / Q000009:adverse effects*; D008297:Male; D009798:Ocular Hypertension / Q000150:complications* / Q000473:pathology; D009900:Optic Nerve / Q000473:pathology*; D011817:Rabbits; D012160:Retina / Q000473:pathology*; D013396:Suction / Q000009:adverse effects