P2X and P2Y purinoceptor expression in pancreas from streptozotocin-diabetic rats.
The expression of the nucleotide receptors P2X1, P2X2, P2X7, P2Y1, P2Y2 and P2Y4, in the pancreas of the streptozotocin-induced diabetic rat was investigated using immunohistochemistry. In diabetic animals, P2X7 receptor expression, normally located in the outer periphery of the islet, was increased and located inside the islet. Double-labelling experiments, using antibodies raised against insulin, somatostatin and glucagon, showed, for the first time, an increase in immunostaining for P2X7 receptors on islet glucagon-containing alpha cells (which had migrated to the interior), while no P2X7 receptors were found in beta and delta cells. P2Y1 receptors were present in intra-islet capillaries, while P2Y4 receptors were found on both alpha and beta cells. P2Y1 and P2Y2 receptor expression was also found in pancreatic duct cells and P2X1, P2X2, P2Y1 and P2Y2 receptors were identified in small blood vessels.
Coutinho-Silva
Robson
R
Autonomic Neuroscience Institute, Royal Free and University College London Medical School, Rowland Hill Street, London NW3 2PF, UK. robson.silva@ucl.ac.uk
Parsons
Mike
M
Robson
Tim
T
Lincoln
Jill
J
Burnstock
Geoffrey
G
D000818:Animals; D002196:Capillaries / Q000737:chemistry; D003921:Diabetes Mellitus, Experimental / Q000473:pathology*; D007150:Immunohistochemistry; D007515:Islets of Langerhans / Q000737:chemistry / Q000166:cytology; D008264:Macrophages / Q000737:chemistry; D008297:Male; D010179:Pancreas / Q000098:blood supply / Q000737:chemistry* / Q000166:cytology; D010183:Pancreatic Ducts / Q000166:cytology; D051381:Rats; D017208:Rats, Wistar; D018048:Receptors, Purinergic P2 / Q000032:analysis*; D058469:Receptors, Purinergic P2X; D058476:Receptors, Purinergic P2X2; D058486:Receptors, Purinergic P2X7; D058472:Receptors, Purinergic P2Y1; D058473:Receptors, Purinergic P2Y2; D013311:Streptozocin
C546403:P2rx2 protein, rat; C546470:P2rx7 protein, rat; C546384:P2ry1 protein, rat; C546387:P2ry2 protein, rat; D018048:Receptors, Purinergic P2; D058469:Receptors, Purinergic P2X; D058476:Receptors, Purinergic P2X2; D058486:Receptors, Purinergic P2X7; D058472:Receptors, Purinergic P2Y1; D058473:Receptors, Purinergic P2Y2; C104749:purinoceptor P2Y4; D013311:Streptozocin