title: [Primary cytomegalovirus infection after combined cadaveric transplantation of the liver and kidney].
abstract: Seronegative transplant recipients are at a high risk of developing primary cytomegalovirus (CMV) infection. The D+/R--constellation produces a 60%-80% probability of CMV disease. In such cases CMV prophylaxis is justified. Presentation of a 12-year old boy who developed a primary CMV infection following A combined liver-kidney transplantation; evaluation of prophylactic options and review of some difficulties in the diagnosis of CMV infection. A cadaveric liver-kidney transplantation (Tx) was done in a 12-year old boy with ESRD due to type I primary hyperoxaluria. CMV status: D+, R-; number of mismatches: 5. PRA 0; kidney cold ischemia time (CIT): 13.54 h; liver CIT: 10.10 h; immediate diuresis; Immunosuppression protocol: anti IL-2 receptor antibodies, steroids, mycophenolate mofetil (MMF); cyclosporine introduced on day 6. Over the first week, daily hemodialyses were done in order to remove oxalate deposits. Kidney and liver biopsies: no ACR, no oxalate deposits. CMV prophylaxis with ganciclovir started on day 0. Routine serology and PCR for CMV follow-up showed: pp 65, IgM and IgG, CMV. DNA (Murex CMV. DNA Hybrid Capture test 2.0): negative over the first 3 months. Day 98: CMV pp 65 positive, IgM neg, DNA neg. Day 108: pp 65 neg, IgM positive, IgG neg. CMV. DNA positive (15 x 105 copies/ml). Clinical status: except for mild Cushing, liver tests and kidney function were normal. Ganciclovir was administered intravenously (i.v.) and after 14 days continued perorally. A few days later, leukopenia with severe neutropenia (neutrophil count: 400) and right otitis media developed. MMF and ganciclovir were withdrawn for a few days and reintroduced after WBC count reconstitution. We had no possibility to monitor MMF. Day 150 pp 65 neg, IgM still positive, IgG neg. No clinical signs of infection. Liver and kidney functions normal. After liver-kidney transplantation in a CMV high-risk pediatric patient (D+/R-), asymptomatic CMV primary infection developed. Although ganciclovir prophylaxis could not prevent the infection, it was mild and delayed. Due to bone marrow suppression, discontinuation of MMF and ganciclovir was necessary. Antigenemia assay pp 65 did not correlate very well with CMV viremia so it could not be recommended as a routine test. It should be used in combination with other CMV tests.
authors.affiliation: Zavod za dijalizu, Klinicki bolnicki centar Zagreb, Kispatićeva 12, 10000 Zagreb, Hrvatska. jasna.slavicek@zg.tel.hr
authors.forename: Jasna
authors.initials: J
authors.lastname: Slavicek
authors.forename: Zvonimir
authors.initials: Z
authors.lastname: Puretić
authors.forename: Snjezana
authors.initials: S
authors.lastname: Glavas-Boras
authors.forename: Ruzica
authors.initials: R
authors.lastname: Smalcelj
authors.forename: Ivan
authors.initials: I
authors.lastname: Barisić
authors.forename: Jurica
authors.initials: J
authors.lastname: Vuković
authors.forename: Durdica
authors.initials: D
authors.lastname: Seso-Simić
authors.forename: Vladimir
authors.initials: V
authors.lastname: Slavicek
authors.forename: Ines
authors.initials: I
authors.lastname: Humar
authors.forename: Petar
authors.initials: P
authors.lastname: Kes
mesh_terms: D002648:Child; D003586:Cytomegalovirus Infections / Q000175:diagnosis / Q000188:drug therapy / Q000209:etiology*; D006801:Humans; D016030:Kidney Transplantation / Q000009:adverse effects*; D016031:Liver Transplantation / Q000009:adverse effects*; D008297:Male