Rev binds specifically to a purine loop in the SL1 region of the HIV-1 leader RNA.
The leader RNA sequence of human immunodeficiency virus type 1 (HIV-1) consists of a complex series of stem loop structures that are critical for viral replication. Three-dimensional structural analysis by NMR of one of these structures, the SL1 stem loop of the packaging signal region, revealed a highly conserved purine rich loop with a structure nearly identical to the Rev-binding loop of the Rev response element. Using band-shift assays, surface plasmon resonance, and further NMR analysis, we demonstrate that this loop binds Rev. HIV-1 appears to have a second Rev-binding site close to the major splice donor site that may have an additional role in the viral life cycle.
Gallego
Jose
J
Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom.
Greatorex
Jane
J
Zhang
Hui
H
Yang
Bin
B
Arunachalam
Shyamala
S
Fang
Jianhua
J
Seamons
John
J
Lea
Susan
S
Pomerantz
Roger J
RJ
Lever
Andrew M L
AM
D020121:5' Untranslated Regions / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D015694:Gene Products, rev / Q000235:genetics / Q000378:metabolism*; D015752:Genes, env; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism*; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D016296:Mutagenesis; D019906:Nuclear Magnetic Resonance, Biomolecular; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017384:Sequence Deletion; D020349:Surface Plasmon Resonance; D054321:rev Gene Products, Human Immunodeficiency Virus
D020121:5' Untranslated Regions; D015694:Gene Products, rev; D012367:RNA, Viral; D054321:rev Gene Products, Human Immunodeficiency Virus