[Molecular characterization of hantavirus Shandong isolate JNL virus strain].
OBJECTIVE
To understand the molecular epidemiologic characteristics of hantavirus Shandong isolate JNL virus strain.


METHODS
The complete M and S gene of the JNL virus isolated from Shandong Province was amplified by RT- PCR, and the purified PCR product was cloned into T vector for sequencing.


RESULTS
The results revealed that the JNL M segment was 3615 bp in length, encoding 1135 amino acids, and the S segment was 1698 bp encoding 429 amino acids, JNL belongs to HTN virus. The comparison of homology with HTN and SEO types showed that the difference of M and S complete sequences between JNL and all other HTN virus strains reached 20.0%-20.6%, and 15.5%-16.0%, respectively. Phylogenetic tree also showed that the position of JNL is located at a different clade.


CONCLUSIONS
HTN virus Shandong local isolate JNL strain is a new specific HTN subtype virus.
Yao
Zhi-hui
ZH
National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China.
Yu
Yong-xin
YX
Dong
Guan-mu
GM
Meng
Xiang-zhi
XZ
Jin
Zhao-hang
ZH
D004279:DNA, Viral / Q000032:analysis; D018063:Hantaan virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006480:Hemorrhagic Fever with Renal Syndrome / Q000821:virology*; D006801:Humans; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D004279:DNA, Viral