Cyclin-dependent kinase inhibitor, roscovitine, in combination with exogenous cytokinin, N6-benzyladenine, causes increase of cis-cytokinins in immobilized tobacco cells.
Dynamics of the response of tobacco cells (line BY-2) to exogenous cytokinin, N6-benzyladenine, and cyclin-dependent kinase inhibitor, roscovitine, was followed using alginate-immobilized cells packed into a column. N6-Benzyladenine (1.25 microM) increased the synthesis of the physiologically-active endogenous cytokinin, isopentenyladenosine, in the effluent up to 0.1 nM. Simultaneously, conversion of the excess of endogenous cytokinins to biologically inactive derivatives of cis-zeatin occurred, up to 0.8 nM. Roscovitine (50 microM) further increased cis-cytokinins, up to 2.2 nM.
Blagoeva
Elitsa
E
Institute of Experimental Botany AS CR, Rozvojová 135, 165 02 Prague 6, Czech Republic.
Malbeck
Jiri
J
Gaudinová
Alena
A
Vanek
Tomás
T
Vanková
Radomíra
R
D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000464:Alginates; D001593:Benzyl Compounds; D002470:Cell Survival; D002478:Cells, Cultured; D018914:Cells, Immobilized / Q000187:drug effects / Q000378:metabolism; D018844:Cyclin-Dependent Kinases / Q000037:antagonists & inhibitors; D003583:Cytokinins / Q000096:biosynthesis; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D007541:Isopentenyladenosine / Q000096:biosynthesis*; D007701:Kinetin; D011687:Purines / Q000494:pharmacology*; D015203:Reproducibility of Results; D000077546:Roscovitine; D012680:Sensitivity and Specificity; D014026:Nicotiana / Q000187:drug effects* / Q000378:metabolism*
D000464:Alginates; D001593:Benzyl Compounds; D003583:Cytokinins; D006603:Hexuronic Acids; D011687:Purines; D000077546:Roscovitine; D007541:Isopentenyladenosine; D020723:Glucuronic Acid; D018844:Cyclin-Dependent Kinases; D000225:Adenine; C480551:benzylaminopurine; D007701:Kinetin