Detection of circovirus with a polymerase chain reaction in the ostrich (Struthio camelus) on a farm in The Netherlands.
This study describes for the first time the presence of circoviruses in ostrich tissue including embryos. A polymerase chain reaction (PCR) was used for the detection of the virus in liver samples. The use of a polymerase for low copy detection significantly increased the sensitivity of the test as well as a Southern blot. Viral DNA could be detected in chicks and eggs that did not hatch. For localisation of the virus in the liver in situ hybridisation was performed on a selection of positive liver tissues.
Eisenberg
S W F
SW
Department of Veterinary Pathology, Utrecht University, Yalelaan 1, 3584 CL, Utrecht, The Netherlands
van Asten
A J A M
AJ
van Ederen
A M
AM
Dorrestein
G M
GM
D000818:Animals; D001483:Base Sequence; D001715:Bird Diseases / Q000821:virology*; D015139:Blotting, Southern / Q000662:veterinary; D018173:Circoviridae Infections / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D017403:In Situ Hybridization / Q000662:veterinary; D008099:Liver / Q000821:virology; D008969:Molecular Sequence Data; D009426:Netherlands; D016133:Polymerase Chain Reaction / Q000662:veterinary; D020310:Struthioniformes
D004279:DNA, Viral