[Determination of four kinds of cell phenotypes of peripheral blood lymphocytes in patients with colorectal cancer].
OBJECTIVE
The functional status of lymphocyte is closely correlated with tumor occurrence and development. The multiple and complicated membranous proteins of lymphocytes are molecular biological foundations by which the cell activities such as membranous information transfer, activation, and apoptosis are proceeded. The purpose of this study was to investigate the expression of phenotypes CD8, CD28, CD49b, and CD49d in peripheral blood lymphocytes (PBL) in the patients with colorectal cancer and explore the relationship between these 4 kinds phenotypes and tumor biological characteristics.


METHODS
Four kinds of cell phenotypes of PBL were measured in 35 patients with colorectal cancer (cancer group) and 30 patients without cancer (control group) using monoclonal antibody and flow cytometry.


RESULTS
The expression of CD8 in cancer and control groups were 30.89+/-6.54(x+/-s, %) and 20.82+/-5.15, respectively. The expression of CD8 in cancer group was significantly higher than that in control group (P< 0.01). The expression of CD28,CD8(+)/CD28(+), CD49b, and CD49d in cancer group and control group were 42.04+/-8.83, 9.29+/-2.81, 22.77+/-10.46, 65.59+/-13.15 and 56.05+/-7.32, 14.91+/-2.58, 33.18+/-17.18,83.61+/-9.14, respectively. The expression of CD28, CD8(+)/CD28(+), CD49b, and CD49d in cancer group were much lower than those in control group(P< 0.01 or P< 0.05). The expression of CD8(+)/CD28(+), CD49b, and CD49b in Dukes D stage and Dukes B stage group were 7.62+/-1.63, 18.53+/-9.59, 59.85+/-16.18 and 10.01+/-2.90, 9.60+/-11.01, 73.12+/-10.86, respectively. The expression of CD8(+)/CD28(+), CD49b, and CD49b in Dukes D stage group were significantly lower than those in Dukes B stage group(P< 0.05). The expression of CD28, CD8(+)/CD28(+), CD49b, and CD49d in lymph node metastasis group and lymph node negative group were 37.62+/-7.38, 9.01+/-2.78, 20.03+/-9.07, 63.19+/-13.10 and 46.63+/-9.04, 13.11+/-2.96, 29.60+/-11.01, 73.12+/-10.86, respectively. The expression of CD28, CD8(+)/CD28(+), CD49b, and CD49d in lymph node metastasis group were much lower than those in lymph node negative group (P< 0.01 or P< 0.05). The expression of CD8 in serosa involved group and no serosa involved group were 34.18+/-3.86 and 28.19+/-5.65, respectively. The expression of CD8 in serosa involved group was higher than that in no serosa involved group(P< 0.01). The expression of CD28, CD8(+)/CD28(+), and CD49d in serosa involved group and no serosa involved group were 39.07+/-7.94, 8.78+/-2.59, 61.32+/-11.95 and 49.27+/-9.54, 12.29+/-3.48, 71.34+/-12.88, respectively. The expression of CD28, CD8(+)/CD28(+), and CD49d in serosa involved group were much lower than those in no serosa involved group (P< 0.01 or P< 0.05).


CONCLUSIONS
Cell phenotypes of CD8, CD28, CD49b, and CD49d of PBL in the patients with colorectal cancer are closely related to tumor invasion and metastasis.
Chen
Teng
T
Department of General Surgery, Changzheng Hospital, The Second Military Medical University, Shanghai, 200003, PR China. tengchen@online.sh.cn
Shi
Jing-Hua
JH
Zhao
Rong-Hua
RH
Li
Hua-Bao
HB
Li
Li
L
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018106:CD28 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D015179:Colorectal Neoplasms / Q000276:immunology* / Q000473:pathology; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D039441:Integrin alpha4 / Q000032:analysis; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging
D018106:CD28 Antigens; D016827:CD8 Antigens; D039441:Integrin alpha4