title: Interactions of antimicrobials in milk and their detection by the disk diffusion method and Delvotest SP.
abstract: The combination of more than 2 different microbials might show interactions with various effects (synergistic, additive, antagonistic, or indifferent) on target microorgnisms. An objective of this paper was to evaluate the possible interactions of several antimicrobials--those used most frequently in the treatment of mastitis in clinical veterinary practice (beta-lactam antibiotics, aminoglycosides, peptides, other antibiotics, and sulfonamides)--and their consequences on detection limits. In the model experiment with milk artificially altered by means of Delvotest SP and the disk diffusion method with Bacillus stearothermophilus var. calidolactis C 953, we observed the synergistic effect between all the antimicrobials tested. The results show that Delvotest SP is more sensitive (approximately 7.5- to 40-fold) than the disk diffusion method in estimating the detection limits of cephalosporin antibiotics.
authors.affiliation: Slovak University of Technology, Faculty of Chemistry and Food Technology, Department of Nutrition and Food Evaluation, Radlinského 9, 812 37 Bratislava, Slovak Republic. kukurova@yahoo.com
authors.forename: Iveta
authors.initials: I
authors.lastname: Kukurová
authors.forename: Bernadetta
authors.initials: B
authors.lastname: Hozová
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis*; D002417:Cattle; D004058:Diffusion; D004350:Drug Residues / Q000032:analysis*; D005260:Female; D008892:Milk / Q000737:chemistry*; D012680:Sensitivity and Specificity
chemical_list: D000890:Anti-Infective Agents