[Evaluation on reducing effect of eye drop on herpes simplex virus-I in vitro].
OBJECTIVE
To observe the effect of herbal eye drop Reduqing (clearing toxic heat) on herpes simplex virus (HSV) on the cytopathic effect in cell culture in vitro.


METHODS
The maximal non-toxic Reduqing eye drop concentration added to cell culture infected with 100 and 10TCID50 of herpes simplex virus? (HSV-1) to observe the inhibitory effect of the medicine on HSV-I induced cytopathic effect.


RESULTS
The Reduqing eye drop at maximal non-toxic concentration could obviously inhibit the cytopatic effect induced by 100TCID50 and 10TCID50 HSV-I. No statistically significant difference was found when compared with 0.1% acyclovir eye drop (P>0.05), on cytopathic effect occurred with boty eye drops. However, there was significant difference when compared with control group(P<0.05). The inhibitory effect of concentrations of Reduqing at 500,000 and 63,000 microg/L was stronger than that of 2,000,000 and 1,000,000 microg/L (P 0.01). The maximal tolerable concentration of Reduqing eye drop by the cell was 2,000,000 microg/L (1:80 dilution), which was higher than that of 5,000 microg/L(1:400 dilution) acyclovir eye drop.


CONCLUSIONS
These results suggest that Reduqing eye drop could markedly inhibit the cytopathic effects caused by HSV-I. The Reduqing eye drop showed remarkably low toxic effect to the cells as compared to acyclovir eye drop.
Zhong
Liang-yu
LY
Department of Ophthalmology, Hangzhou Hospital of Traditional Chinese Medicine, Hangzhou 310007, China.
Chen
Jing-yi
JY
Chen
Hong-mei
HM
Xu
Wei
W
Wang
Xiao-meng
XM
Lai
Jian
J
Liu
Ya-qin
YQ
D000998:Antiviral Agents / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D018259:Herpesvirus 1, Human / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D009883:Ophthalmic Solutions
D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; D009883:Ophthalmic Solutions