title: Regeneration of peroxiredoxins during recovery after oxidative stress: only some overoxidized peroxiredoxins can be reduced during recovery after oxidative stress.
abstract: Peroxiredoxins (prx) are redox enzymes using an activated cysteine as their active site. This activated cysteine can be easily overoxidized to cysteine sulfinic acid or cysteine sulfonic acid, especially under oxidative stress conditions. The regeneration of peroxiredoxins after a short, intense oxidative stress was studied, using a proteomics approach. Important differences in regeneration speed were found, prx2 being the fastest regenerated protein, followed by prx1, whereas prx3 and prx6 were regenerated very slowly. Further study of the mechanism of this regeneration by pulse-chase experiments using stable isotope labeling and cycloheximide demonstrated that the fast-regenerating peroxiredoxins are regenerated at least in part by a retroreduction mechanism. This demonstrates that the overoxidation can be reversible under certain conditions. The pathway of this retroreduction and the reasons explaining the various regeneration speeds of the peroxiredoxins remain to be elucidated.
authors.affiliation: Commissariat à l'Energie Atomique-Laboratoire de Bioénergétique Cellulaire et Pathologique, EA 2943, Département Réponses et Dynamique Cellulaire/BioEnergétique Cellulaire et Pathologique-Grenoble, 17 rue des martyrs, F-38054 Grenoble Cedex 9, France.
authors.forename: Mireille
authors.initials: M
authors.lastname: Chevallet
authors.forename: Elsa
authors.initials: E
authors.lastname: Wagner
authors.forename: Sylvie
authors.initials: S
authors.lastname: Luche
authors.forename: Alain
authors.initials: A
authors.lastname: van Dorsselaer
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Leize-Wagner
authors.forename: Thierry
authors.initials: T
authors.lastname: Rabilloud
mesh_terms: D001665:Binding Sites; D006367:HeLa Cells; D006801:Humans; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010544:Peroxidases / Q000378:metabolism*; D054464:Peroxiredoxins; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D010544:Peroxidases; D054464:Peroxiredoxins