Inhibition of polyamine biosynthesis in Acanthamoeba castellanii and 12-O-tetradecanoylphorbol-13-acetate-induced ornithine decarboxylase activity by chitosanoligosaccharide.
Growth of Acanthamoeba castellanii was inhibited by chitosanoligosaccharide (up to 20 mg ml-1) from the shells of crabs but was reversed by the polyamines, putrescine or spermidine, at 0.8 mM. Chitosanoligosaccharide strongly inhibited the induction of ornithine decarboxylase by 12-O-tetradecanoylphorbol-13-acetate, a key enzyme of polyamine biosynthesis, which is enhanced in tumour promotion.
Shon
Yun-Hee
YH
Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University, Kyongju 780-714, Korea.
Nam
Kyung-Soo
KS
D000048:Acanthamoeba / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D002686:Chitin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology*; D048271:Chitosan; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D004817:Epidermis / Q000187:drug effects / Q000201:enzymology*; D051379:Mice; D009844:Oligosaccharides / Q000138:chemical synthesis / Q000494:pharmacology*; D009955:Ornithine Decarboxylase / Q000378:metabolism; D065108:Ornithine Decarboxylase Inhibitors; D011073:Polyamines / Q000378:metabolism*; D011700:Putrescine; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013095:Spermidine; D013755:Tetradecanoylphorbol Acetate / Q000031:analogs & derivatives* / Q000493:pharmacokinetics
D009844:Oligosaccharides; D065108:Ornithine Decarboxylase Inhibitors; D011073:Polyamines; D002686:Chitin; C020922:4-O-methyl-12-O-tetradecanoylphorbol 13-acetate; D048271:Chitosan; D009955:Ornithine Decarboxylase; D013755:Tetradecanoylphorbol Acetate; D013095:Spermidine; D011700:Putrescine