title: Spectra and lifetimes of fluorescence resonance energy transfer fluorophores under two-photon excitation.
abstract: We show two-photon spectra and lifetimes acquired using conventional confocal microscopes equipped with an ultra-short pulsed laser and a time-gated intensified charge coupled device. We report on the two-photon spectra and lifetimes of Alexa350, enhanced green fluorescent protein (EGFP), EGFP-CD46, and Cy3 labelled antibodies. Cellular and extracellular EGFP two-photon spectra and lifetimes are compared.
authors.affiliation: Centre for Micro-Photonics, School of Biophysical Sciences and Electronic Engineering, Swinburne University of Technology, Hawthorn, Australia.
authors.forename: Ross
authors.initials: R
authors.lastname: Ashman
authors.forename: Blessing
authors.initials: B
authors.lastname: Crimeen-Irwin
authors.forename: Mandy
authors.initials: M
authors.lastname: Ludford-Menting
authors.forename: Sarah
authors.initials: S
authors.lastname: Russell
authors.forename: Min
authors.initials: M
authors.lastname: Gu
mesh_terms: D000085:Acetates / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000378:metabolism; D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism; D019556:COS Cells; D002232:Carbocyanines / Q000737:chemistry / Q000378:metabolism; D002867:Chromones / Q000737:chemistry / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism*; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000737:chemistry / Q000378:metabolism; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins / Q000276:immunology / Q000378:metabolism; D008856:Microscopy, Fluorescence; D017785:Photons
chemical_list: D000085:Acetates; C400304:Alexa 350; D000906:Antibodies; D015703:Antigens, CD; D002232:Carbocyanines; D002867:Chromones; D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins; C089187:cyanine dye 3; D049452:Green Fluorescent Proteins