title: Autoantibodies and histogenesis of celiac disease.
abstract: OBJECTIVE
  Autoantibodies are used as markers for celiac disease (CD) identifying patients with mucosal lesions. The purpose of this study was to evaluate the sensitivity and role of the autoantibodies such as IgA antiendomysium (EMA), IgA antigliadin (AGA) and the IgA antitissue transglutaminase (tTGA) in histogenesis of celiac disease.
  
  
  METHODS
  Seventy-nine cases including 30 untreated celiacs, 5 celiacs on gluten-free diet (GFD), 41 first degree relatives and 3 non-relatives suspected for CD were investigated. Three untreated celiacs with IgA deficiency were excluded from this study group. IgA antibodies to tTGA were determined by ELISA, as described before. Twelve of 41 relatives and 2 cases of non relatives suspected with positive serology underwent a small intestinal biopsy. Results were correlated with the degrees of abnormality of the intestinal mucosa in patients with CD. Intestinal biopsies obtained from study population were evaluated for histological quantification.
  
  
  RESULTS
  Celiacs and suspected cases with positive EMA/AGA and or tTGA showed shorter villi (p < 0.007) and/or a higher number of intraepithelial lymphocytes (IEL) (p < 0.035). The sensitivity of serology (EMA, AGA, tTGA) in patients with Marsh IIIc was 100%. However, in patients with Marsh IIIa the sensitivity for EMA, AGA, and tTGA was 40%, 50% and 20% respectively.
  
  
  CONCLUSIONS
  The appearance of antibodies is related to the degree of mucosal infiltration by IELs. Although tTGA, like EMA provide a highly sensitive parameter for the detection of celiacs with severe mucosal damage, it appears to be less sensitive (even less than AGA) in celiac patients with milder histopathological abnormalities. However, it should be recognized that the substantial part of the celiac population present with these milder forms of mucosal abnormalities. Using tTGA as a single test in screening may result in missing up to 60-70% of celiacs with mild mucosal abnormalities. Combination with other screening tests (at least with AGA) is essential and strongly recommended
authors.affiliation: Department of Gastroenterology Withybush General Hospital, Pembrokeshire, Haverfordwest, UK. krostami@hotmail.com.
authors.forename: Kamran
authors.initials: K
authors.lastname: Rostami
authors.forename: Chris J J
authors.initials: CJ
authors.lastname: Mulder
authors.forename: Steven
authors.initials: S
authors.lastname: Stapel
authors.forename: B Mary E
authors.initials: BM
authors.lastname: von Blomberg
authors.forename: Jo
authors.initials: J
authors.lastname: Kerckhaert
authors.forename: Jos W R
authors.initials: JW
authors.lastname: Meijer
authors.forename: Salvador A
authors.initials: SA
authors.lastname: Peńa
authors.forename: Hugo S A
authors.initials: HS
authors.lastname: Heymans
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000097:blood*; D002446:Celiac Disease / Q000175:diagnosis* / Q000276:immunology* / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D004386:Duodenum / Q000473:pathology*; D004797:Enzyme-Linked Immunosorbent Assay; D005903:Gliadin / Q000276:immunology*; D006801:Humans; D008875:Middle Aged; D011503:Transglutaminases / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D005903:Gliadin; D011503:Transglutaminases