title: Monocytes and neutrophils from tuberculosis patients are insensitive to anti-inflammatory effects triggered by the prototypic formyl peptide N-formyl-methionyl-leucyl-phenylalanine (FMLP).
abstract: Tuberculosis is a chronic infectious disease caused by Mycobacterium tuberculosis where formyl peptides, which are cleavage products of bacterial and mitochondrial proteins, are present. In this study, we demonstrated that interferon gamma (IFN)-gamma and interleukin (IL)-10 induced the overexpression of the receptor for the Fc portion of IgG I (FcgammaRI) in monocytes from tuberculosis (TB) patients, showing that these cells respond to IFN-gamma and IL-10 signals. We also demonstrated that lower doses of IL-10 render monocytes from TB patients less responsive to higher doses of the cytokine. Although the prototypic formyl peptide N-formyl-methionyl-leucyl-phenylalanine (FMLP) is a well-known proinflammatory agonist, we have demonstrated previously that preincubation of monocytes with FMLP inhibited the up-regulation of FcgammaRI induced by IFN-gamma or IL-10. This effect was not observed in monocytes from TB patients. FMLP also induced the down-regulation of the expression of FcgammaRI in monocytes that had been activated already with IFN-gamma. However, this effect of FMLP was not observed in monocytes from TB patients and supernatants from monocytes obtained from these patients were incapable of inducing the down-regulation of FcgammaRI. In contrast to normal donors, supernatants from FMLP-treated neutrophils from TB patients did not modify the basal level of expression of FcgammaRI in monocytes from normal donors. In conclusion, in this study we demonstrated the existence of two novel mechanisms that may contribute to the pathological effects generated by M. tuberculosis: the enhancement of FcgammaRI in response to IFN-gamma and IL-10, and the unresponsiveness to the anti-inflammatory effects induced by formyl peptides.
authors.affiliation: División Inmunología, Instituto de Investigaciones Hematológicas, Academia Nacional de Medicina, Hospital F. J. Muñiz, Buenos Aires, Argentina. mbeigier@yahoo.com
authors.forename: M
authors.initials: M
authors.lastname: Beigier-Bompadre
authors.forename: M
authors.initials: M
authors.lastname: Alemán
authors.forename: P
authors.initials: P
authors.lastname: Barrionuevo
authors.forename: M C
authors.initials: MC
authors.lastname: Franco
authors.forename: C J
authors.initials: CJ
authors.lastname: Rubel
authors.forename: M Del C
authors.initials: Mdel C
authors.lastname: Sasiain
authors.forename: M S
authors.initials: MS
authors.lastname: Palermo
authors.forename: E
authors.initials: E
authors.lastname: Abbate
authors.forename: M A
authors.initials: MA
authors.lastname: Isturiz
mesh_terms: D002478:Cells, Cultured; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology; D016753:Interleukin-10 / Q000276:immunology; D008297:Male; D009000:Monocytes / Q000187:drug effects / Q000276:immunology*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D017452:Receptors, IgG / Q000378:metabolism; D014397:Tuberculosis, Pulmonary / Q000276:immunology*; D015854:Up-Regulation / Q000276:immunology
chemical_list: D017382:Reactive Oxygen Species; D017452:Receptors, IgG; D016753:Interleukin-10; D009240:N-Formylmethionine Leucyl-Phenylalanine; D007371:Interferon-gamma