Change of porcine circovirus 2 target cells in pigs during development from fetal to early postnatal life.
Change of porcine circovirus 2 (PCV2) target cells during development from fetal to postnatal life in pigs was examined. PCV2 inoculation was performed in fetuses in utero at either 57, 75 or 92 gestational days and in piglets at 1 day of age. Twenty-one days after virus inoculation, PCV2-infected cells in the heart, lungs, liver, spleen and inguinal lymph nodes were localized and immuno-phenotyped by double-immunofluorescence labeling using different cell markers and PCV2-antibodies. During fetal life, viral antigens were detected in cardiomyocytes, hepatocytes and macrophages and infected cell numbers decreased with increasing fetal age at inoculation. The heart contained the highest number of infected cells and cardiomyocytes were the main target cell. Postnatally, macrophages were the only target cell type in different organs and infected cell numbers were similar to those of fetuses inoculated at 92 days of gestation. One piglet showed exceptionally high number of infected cells in different organs with values 13-513-fold higher compared to littermates. In this piglet, the majority of infected cells in lymphoid tissues could not be typed. This study reveals that PCV2 target cells change from cardiomyocytes, hepatocytes and macrophages during fetal life to only macrophages postnatally.
Sanchez
Romeo E
RE
Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
Meerts
Peter
P
Nauwynck
Hans J
HJ
Pensaert
Maurice B
MB
D000818:Animals; D000831:Animals, Newborn; D000956:Antigens, Viral / Q000378:metabolism; D018173:Circoviridae Infections / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000254:growth & development*; D005333:Fetus; D006321:Heart / Q000821:virology; D022781:Hepatocytes / Q000821:virology; D008099:Liver / Q000821:virology; D008168:Lung / Q000821:virology; D008198:Lymph Nodes / Q000821:virology; D008264:Macrophages / Q000821:virology; D008856:Microscopy, Fluorescence / Q000662:veterinary; D032383:Myocytes, Cardiac / Q000821:virology; D013154:Spleen / Q000821:virology; D013552:Swine / Q000196:embryology / Q000254:growth & development / Q000821:virology*; D013553:Swine Diseases / Q000821:virology*
D000956:Antigens, Viral