The secretory response through electric stimulation of differentiated PC12 rat pheochromocytoma cells transfected with neuropeptide Y fused with enhanced green fluorescent protein.
Exocytosis in pheochromocytoma cells was induced by electric stimulation. To chase the movement of vesicles by electric stimulation, dense-core secretory vesicles were visualized by expression of the fusion protein between neuropeptide Y and enhanced green fluorescent protein (EGFP) in these differentiated PC12 rat pheochromocytoma cells. When the cells were stimulated with constant voltage potential at -300 mV, the movement of dense-core secretory vesicles could be regulated.
Mizuno
Atsushi
A
Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.
Mie
Masayasu
M
Yanagida
Yasuko
Y
Aizawa
Masuo
M
Kobatake
Eiry
E
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D004558:Electric Stimulation; D005089:Exocytosis / Q000187:drug effects / Q000502:physiology; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000096:biosynthesis / Q000235:genetics*; D016501:Neurites / Q000378:metabolism; D009478:Neuropeptide Y / Q000235:genetics*; D016716:PC12 Cells; D010673:Pheochromocytoma / Q000188:drug therapy / Q000378:metabolism*; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000378:metabolism*; D022142:Secretory Vesicles / Q000378:metabolism; D013268:Stimulation, Chemical; D013572:Synaptic Vesicles / Q000378:metabolism; D014162:Transfection
D008164:Luminescent Proteins; D009478:Neuropeptide Y; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D011189:Potassium Chloride