Quantitative determination of sugars and myo-inositol in citrus fruits grown in Japan using high-performance anion-exchange chromatography.
Fifteen commercial samples of citrus fruits grown in Japan were analyzed for their sugar contents by high-performance anion-exchange chromatography (HPAEC) and electrochemical detection (ED) coupled with a stationary phase D10 column prepared using chloromethylated styrene-divinylbenzene copolymer and N,N,N',N'-tetramethyldiaminodecane. Myo-inositol, glucose, fructose, and sucrose in all of these various citrus fruits could be successfully separated within 25 min using 0.5 M NaOH eluent at a flow-rate of 0.4 mL/min. Myo-inositol, as a better nutritional source, was found in all the citrus fruits grown in Japan (0.7 +/- 0.04-2.1 +/- 0.2 g/L). The sugar contents of twelve citrus fruits grown in other countries were also determined.
Masuda
Takako
T
Department of Chemistry, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo 160-8402, Japan. m-chem@tokyo-med.ac.jp
Kawano
Ami
A
Kitahara
Kei-ichi
K
Nagashima
Kunio
K
Aikawa
Yoshihiro
Y
Arai
Sadao
S
D002241:Carbohydrates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D002957:Citrus / Q000737:chemistry*; D005632:Fructose / Q000032:analysis; D005638:Fruit / Q000737:chemistry*; D005947:Glucose / Q000032:analysis; D007294:Inositol / Q000032:analysis*; D007564:Japan; D013395:Sucrose / Q000032:analysis
D002241:Carbohydrates; D005632:Fructose; D007294:Inositol; D013395:Sucrose; D005947:Glucose