Lactobacillus gasseri OLL2716 as a probiotic in clarithromycin-resistant Helicobacter pylori infection.
OBJECTIVE
Clarithromycin (CAM)-resistant Helicobacter pylori sometimes offers serious problems with eradication by antibiotics. The aim of this study was to determine whether a probiotic can be an alternative therapy in CAM-resistant Hp infection.


METHODS
The effects of Lactobacillus gasseri (strain OLL2716) on the growth of CAM-susceptible and CAM-resistant H. pylori and interleukin (IL)-8 production provoked by these strains were examined by in vitro experiments. Moreover, mice were infected with these CAM-susceptible or CAM-resistant H. pylori, and were treated with CAM or L. gasseri.


RESULTS
In vitro experiments demonstrated that L. gasseri inhibited the growth of H. pylori and suppressed H. pylori-associated IL-8 production. Such effects were noted in CAM-resistant and CAM-susceptible H. pylori. Similarly, in an in vivo model of H. pylori infection, H. pylori colonization was significantly decreased by L. gasseri.


CONCLUSIONS
Therefore, L. gasseri was found to act as a probiotic in CAM-resistant H. pylori infection.
Ushiyama
Atsushi
A
Department of Infectious Diseases, Tokai University School of Medicine, Bohseidai, Isehara, Japan.
Tanaka
Kazuo
K
Aiba
Yuji
Y
Shiba
Tadahiko
T
Takagi
Atsushi
A
Mine
Tetsuya
T
Koga
Yasuhiro
Y
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017291:Clarithromycin / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D016481:Helicobacter Infections / Q000188:drug therapy*; D016480:Helicobacter pylori / Q000187:drug effects*; D016209:Interleukin-8 / Q000378:metabolism; D007778:Lactobacillus; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008826:Microbial Sensitivity Tests; D019936:Probiotics / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D016209:Interleukin-8; D017291:Clarithromycin