title: Effects of age on morphology, protein synthesis and secretagogue-evoked secretory responses in the rat lacrimal gland.
abstract: This study investigated changes in the morphology and protein synthesis and protein and peroxidase secretion due to peptidergic and aminergic stimulation from rat lacrimal gland acinar cells of 3-5, 9, 12, 20 and 24 month old rats. There was a marked reduction in the presence of Golgi apparatus in the acinar cells of glands from the 24 month old rats coupled to dilatation and degeneration of rough endoplasmic reticulum, when compared to that in the acinar cells of glands from 3-5 and 12 month old rats. Following incorporation of tritiated leucine for 360 min (6 h), the amount of newly synthesised protein in acinar cells of the 12 month old rats was significantly (p < 0.01) higher than that in the acinar cells of 3-5 month old animals. However, at 20 months the amount of newly synthesised protein in these acinar cells was significantly (p < 0.01) reduced to less than that in acinar cells of both the 3-5 and 12 month old animals. Immunohistochemical and immunofluorescence studies identified the presence of substance P (SP), vasoactive intestinal peptide (VIP), histamine and 5-hydroxytryptamine (5-HT) in the lacrimal glands of 3-5 month old rats. Stimulation by either SP, VIP, histamine or 5-HT resulted in significant increases in total protein output and peroxidase release from acinar cells of the 3-5 month old rats. However, all responses to the secretagogues were reduced with ageing from 3-5 to 24 months of age. The results indicate that ageing is associated with alteration in the ability of acinar cells to synthesise and secrete proteins.
authors.affiliation: Department of Biological Sciences, University of Central Lancashire, Preston, UK.
authors.forename: Clare E
authors.initials: CE
authors.lastname: Draper
authors.forename: Jaipaul
authors.initials: J
authors.lastname: Singh
authors.forename: Ernest
authors.initials: E
authors.lastname: Adeghate
mesh_terms: D000367:Age Factors; D000375:Aging; D000818:Animals; D003593:Cytoplasm / Q000378:metabolism; D018870:Endoplasmic Reticulum, Rough / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism; D006632:Histamine / Q000096:biosynthesis / Q000494:pharmacology; D007150:Immunohistochemistry; D007765:Lacrimal Apparatus / Q000378:metabolism*; D007930:Leucine / Q000737:chemistry / Q000378:metabolism; D008297:Male; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D010544:Peroxidases / Q000378:metabolism; D014176:Protein Biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000096:biosynthesis / Q000494:pharmacology; D013373:Substance P / Q000096:biosynthesis / Q000494:pharmacology; D013997:Time Factors; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism / Q000494:pharmacology
chemical_list: D012701:Serotonin; D013373:Substance P; D014660:Vasoactive Intestinal Peptide; D006632:Histamine; D010544:Peroxidases; D007930:Leucine