Preventive effect of hydrotalcite on gastric mucosal injury in rats induced by taurocholate.
OBJECTIVE
To study the preventive effect of hydrotalcite on gastric mucosal injury in rat induced by taurocholate, and to investigate the relationship between the protective mechanism of hydrotalcite and the expression of trefoil factor family 2 (TFF2) mRNA and c-fos protein.


METHODS
Forty five male Wistar rats were randomly divided into hydrotalcite group, ranitidine group and control group. Gastric mucosal injury was induced by introgastric acidified taurocholate. OD value of TFF2 mRNA expression in gastric mucous cells was determined by hybridization and computer image analysis system. OD value of c-fos protein expression in gastric mucous cells was measured by immunohistochemistry and computer image analysis system.


RESULTS
The gross mucosal injury index in hydrotalcite group was significantly lower than that in ranitidine group and control group (8.60+/-2.20 vs 16.32+/-4.27, 29.53+/-5.39; P<0.05, P<0.01). The expression level of TFF2 mRNA in hydrotalcite group was markedly higher than that in ranitidine group and control group (0.56+/-0.09 vs 0.30+/-0.05, 0.28+/-0.03, P<0.05). The OD value of c-fos protein in hydrotalcite group was higher than that in ranitidine group and control group (0.52+/-0.07 vs 0.31+/-0.04, 0.32+/-0.05, P<0.05).


CONCLUSIONS
Hydrotalcite can protect gastric mucosal injury in rats induced by taurocholate, which may be related to the increased expression of TFF2 and c-fos protein.
Yu
Bao-Ping
BP
Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China. yubaoping62@yahoo.com.cn
Sun
Jun
J
Li
Mu-Qi
MQ
Luo
He-Sheng
HS
Yu
Jie-Ping
JP
D000536:Aluminum Hydroxide / Q000494:pharmacology*; D000818:Animals; D000863:Antacids / Q000494:pharmacology*; D002756:Cholagogues and Choleretics; D004487:Edema / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control; D005753:Gastric Mucosa / Q000187:drug effects / Q000473:pathology; D006940:Hyperemia / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control; D008276:Magnesium Hydroxide / Q000494:pharmacology*; D008297:Male; D009077:Mucins; D009124:Muscle Proteins; D009479:Neuropeptides; D010455:Peptides / Q000235:genetics; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D013272:Stomach Diseases / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D013656:Taurocholic Acid; D000071164:Trefoil Factor-2; D000071165:Trefoil Factor-3
D000863:Antacids; D002756:Cholagogues and Choleretics; D009077:Mucins; D009124:Muscle Proteins; D009479:Neuropeptides; D010455:Peptides; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; C099037:TFF3 protein, rat; C000604008:Tff2 protein, rat; D000071164:Trefoil Factor-2; D000071165:Trefoil Factor-3; C010467:hydrotalcite; D013656:Taurocholic Acid; D000536:Aluminum Hydroxide; D008276:Magnesium Hydroxide