Optimization of growth conditions of the postharvest biocontrol agent Candida sake CPA-1 in a lab-scale fermenter.
OBJECTIVE
To maximize the growth (expressed as number of viable cells per millilitre) of the postharvest biocontrol agent Candida sake CPA(-1) at laboratory scale conditions.


RESULTS
Growth conditons (aeration, agitation speed and inoculum size) were studied in batch conditions in a 5 l fermenter using molasses and urea as growth medium. Consumption of sugars and urea were analysed. Fed-batch studies were also carried out. Glucose and fructose were consumed during the exponential growth phase and were depleted after 18 h of growth. On the contrary, C. sake cells assimilated sucrose during the stationary phase. There was not growth improvement when fed-batch technology was used. Addition of an extra amount of glucose or molasses after 18 h of growth did not contribute to increase final population.


CONCLUSIONS
Maximum growth (about 8 x 10(8 )CFU ml(-1)) was obtained at batch fermentation after 30 h growth at 400 rev min(-1), 150 l h(-1) of air and initial concentration of 106 CFU ml(-1).


CONCLUSIONS
The results obtained in this study are an approach for further upscaling of C. sake production.
Abadias
M
M
Postharvest Area, CeRTA, Centre UdL-IRTA, 177 Rovira Roure, 25198 Lleida, Catalonia, Spain. isabel.abadias@irta.es
Teixidó
N
N
Usall
J
J
Viñas
I
I
D000388:Air; D002175:Candida / Q000254:growth & development* / Q000378:metabolism; D003114:Colony-Forming Units Assay; D003470:Culture Media; D005285:Fermentation; D005632:Fructose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D010572:Pest Control, Biological; D013395:Sucrose / Q000378:metabolism; D014508:Urea / Q000378:metabolism
D003470:Culture Media; D005632:Fructose; D013395:Sucrose; D014508:Urea; D005947:Glucose