DNA topoisomerases I and II inhibitory activity of constituents isolated from Juglans mandshurica.
Nine diarylheptanoids (1-9), one triterpene (10), one sesquiterpenoid (11), one naphthoquinone (12), four tetralones (13-16), one naphthalene carboxylic acid glucoside (17) and six naphthalenyl glycosides (18-23) were isolated from the roots of Juglans mandshurica Maximowicz (Juglandaceae), and their structures determined from the chemical and spectral data. Here, we report the inhibitory effects, on the DNA topoisomerases I and II activities, of all these compounds. Compounds 10 and 23 showed more potent inhibitory effects, on the DNA topoisomerases I and II (94.0 and 86.0% inhibitions at the concentration of 5 microg/mL, respectively), than the positive control compounds, camptothecin and etoposide.
Li
Gao
G
College of Pharmacy, Yeungnam University, Gyongsan 712-749, Korea.
Lee
Sun-Young
SY
Lee
Kyeung-Seon
KS
Lee
Sung-Won
SW
Kim
Sang-Hyun
SH
Lee
Seung-Ho
SH
Lee
Chong-Soon
CS
Woo
Mi-Hee
MH
Son
Jong-Keun
JK
D000818:Animals; D002417:Cattle; D045744:Cell Line, Tumor / Q000187:drug effects / Q000201:enzymology; D004264:DNA Topoisomerases, Type I / Q000378:metabolism; D004250:DNA Topoisomerases, Type II / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D031324:Juglans; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018517:Plant Roots; D059004:Topoisomerase I Inhibitors; D059005:Topoisomerase II Inhibitors
D004791:Enzyme Inhibitors; D010936:Plant Extracts; D059004:Topoisomerase I Inhibitors; D059005:Topoisomerase II Inhibitors; D004264:DNA Topoisomerases, Type I; D004250:DNA Topoisomerases, Type II