title: Murine lymph node-derived stromal cells effectively support survival but induce no activation/proliferation of peripheral resting T cells in vitro.
abstract: Little is known about the homeostatic mechanisms by which the levels of peripheral lymphocytes are maintained. The survival of naïve T cells in vivo must be maintained by some factors that have not been characterized in an in vitro culture system. In this study, we established a culture system of stromal cells derived from murine lymph nodes and investigated the action of the stromal cells in supporting the survival of resting T cells in vitro. Most of the T cells cocultured with the stromal cells did not die, and the supernatant of cultured stromal cells increase the viability of T cells. This T-cell survival-supporting activity was maintained for more than 7 days. Although interleukin (IL)-4, IL-6, IL-7, and interferon-beta also rescued peripheral T cells from spontaneous cell death, medium-soluble and heat-sensitive factor(s) derived from the stromal cells supported the survival of T cells more effectively and for a longer time than did these cytokines. T cells maintained in the culture system with the stromal cells appeared to remain in a resting G0/G1 state and did not show remarkable DNA synthesis. From these results, it is presumed that some soluble factor(s) other than the tested cytokines that have been identified as supporting T-cell survival are produced from lymph node stromal cells. These factor(s) play an important role in maintenance of resting T cells.
authors.affiliation: Departments of Immunology and Equipment Center for Research and Education, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Yan-Wen
authors.initials: YW
authors.lastname: Zhou
authors.forename: Sayoko
authors.initials: S
authors.lastname: Aritake
authors.forename: Agustina
authors.initials: A
authors.lastname: Tri Endharti
authors.forename: Jianghong
authors.initials: J
authors.lastname: Wu
authors.forename: Akemi
authors.initials: A
authors.lastname: Hayakawa
authors.forename: Izumi
authors.initials: I
authors.lastname: Nakashima
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000276:immunology; D002455:Cell Division; D002470:Cell Survival; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology; D004247:DNA / Q000096:biosynthesis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D015870:Gene Expression / Q000235:genetics; D019254:Genes, bcl-2 / Q000235:genetics; D015151:Immunoblotting; D008198:Lymph Nodes / Q000166:cytology* / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D017154:Stromal Cells / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D051020:bcl-X Protein
chemical_list: C494812:Bcl2l1 protein, mouse; D016207:Cytokines; D019253:Proto-Oncogene Proteins c-bcl-2; D051020:bcl-X Protein; D004247:DNA