Determination and use of haplotypes: ethnic comparison and association of the lipoprotein lipase gene and coronary artery disease in Mexican-Americans.
OBJECTIVE
To illustrate an approach of deriving haplotypes for genetic association studies, using the lipoprotein lipase (LPL) gene and coronary artery disease.


METHODS
Six polymorphisms sufficient to distinguish the most common haplotypes in the 3' end of LPL were identified by genotyping 10 polymorphisms in a small pilot population. These were used to haplotype LPL in large family samples of Mexican-Americans and non-Hispanic Caucasians. A case-control association study was performed comparing Mexican-Americans with and without coronary artery disease.


RESULTS
The two ethnic groups exhibited significant genetic differences. Among Mexican-Americans, homozygosity for LPL haplotype 1 was protective against coronary artery disease (OR = 0.50, 95% CI 0.27-0.91).


CONCLUSIONS
This study outlines the haplotype structure of the LPL gene, illustrates the utility of haplotype-based analysis in association studies, and demonstrates the importance of defining haplotype frequencies for different ethnic groups.
Goodarzi
Mark O
MO
Division of Medical Genetics, Department of Medicine, Cedars-Sinai Medical Center and UCLA, Los Angeles, California, USA.
Guo
Xiuqing
X
Taylor
Kent D
KD
Quiñones
Manuel J
MJ
Samayoa
Carlos
C
Yang
Huiying
H
Saad
Mohammad F
MF
Palotie
Aarno
A
Krauss
Ronald M
RM
Hsueh
Willa A
WA
Rotter
Jerome I
JI
D000483:Alleles; D003324:Coronary Artery Disease / Q000208:ethnology* / Q000235:genetics*; D005787:Gene Frequency; D005838:Genotype; D006239:Haplotypes; D006630:Hispanic or Latino; D006720:Homozygote; D006801:Humans; D008071:Lipoprotein Lipase / Q000235:genetics*; D016015:Logistic Models; D017058:Mexican Americans; D011110:Polymorphism, Genetic; D044465:White People
D008071:Lipoprotein Lipase