A quantitative method for measuring the mass concentration of the filamentous bacterium Type 021N in activated sludge using fluorescence in situ hybridization.
OBJECTIVE
This study aimed to develop a quantitative method for measuring mass concentrations of Type 021N, a bacterium causing bulking in activated sludge.


RESULTS
Fluorescence in situ hybridization was used to determine the relationship between the concentration ratio of the mass of the bacterium Type 021N to mass of activated sludge, and the proportion of fluorescence area imparted by probe G123T specific for Type 021N to that obtained with probe EUB338 for bacteria. A linear relationship existed between the cube root of the mass concentration ratio and square root of this area proportion.


CONCLUSIONS
A standard curve was obtained for quantifying Type 021N in activated sludge.


CONCLUSIONS
This method may allow the determination of growth rate constant of filamentous bacteria in activated sludge, information that will help in understanding their ecology.
Guan
Y
Y
Institute of Environmental Studies, Graduate school of Frontier Sciences, The University of Tokyo, Tokyo, Japan. guanyt@mail.tsinghua.edu.cn
Kurisu
F
F
Satoh
H
H
Mino
T
T
D001419:Bacteria / Q000235:genetics* / Q000302:isolation & purification*; D001431:Bacteriological Techniques / Q000592:standards; D001483:Base Sequence; D018533:Biomass; D015342:DNA Probes / Q000235:genetics; D005456:Fluorescent Dyes; D017404:In Situ Hybridization, Fluorescence / Q000379:methods* / Q000592:standards; D018613:Microscopy, Confocal; D012722:Sewage / Q000382:microbiology*
D015342:DNA Probes; D005456:Fluorescent Dyes; D012722:Sewage