title: Tumor cells expressing membrane-bound tumor necrosis factor activate macrophages and have a compromised growth in immunosuppressed and immunodeficient mice.
abstract: Tumor necrosis factor (TNF)-alpha producing tumors as vaccines were demonstrated to induce a therapeutic anti-tumor immune response, but their clinical use is limited by the toxicity of soluble TNF. We investigated the growth characteristics and immunomodulatory properties of HeLa cells producing an uncleavable transmembrane form of TNF (preTNF). The growth of the transformed tumors was compromised in both immunosuppressed and severe combined immunodeficient mice; no signs of TNF toxicity were detected. Macrophages co-cultured with the transformed cells showed increased phagocytosis and cytokine production, indicating that activated macrophages may be the mediators of the anti-tumor effect. preTNF producing tumor cells are promising safe anti-tumor vaccine candidates.
authors.affiliation: Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, Szeged, Hungary.
authors.forename: Tünde
authors.initials: T
authors.lastname: Nagy
authors.forename: Hristos
authors.initials: H
authors.lastname: Glavinas
authors.forename: Nóra
authors.initials: N
authors.lastname: Szincsák
authors.forename: János
authors.initials: J
authors.lastname: Hunyadi
authors.forename: Tamás
authors.initials: T
authors.lastname: Jánossy
authors.forename: Ernö
authors.initials: E
authors.lastname: Duda
authors.forename: Csaba
authors.initials: C
authors.lastname: Vizler
authors.forename: István
authors.initials: I
authors.lastname: Juhász
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000737:chemistry; D018920:Coculture Techniques; D005260:Female; D006367:HeLa Cells; D006801:Humans; D007108:Immune Tolerance; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008808:Mice, Inbred CBA; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D010587:Phagocytosis; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000276:immunology
chemical_list: D014409:Tumor Necrosis Factor-alpha