title: Inhibition on telomerase activity and cytotoxic effects by cisplatin in cultured human choroidal melanoma cells.
abstract: OBJECTIVE
  To study the changes of telomerase activity and cytotoxic effects by Cisplatin; cis-dichlorodiamine platinum (CDDP) in cultured human choroidal melanoma.
  
  
  METHODS
  The primary cultured human choroidal melanoma cells were cultured in the presence and absence of CDDP with different concentration and time respectively. The toxic effects were evaluated by MTT and the level of telomerase was detected by PCR-ELISA assay. And the relationship between telomerase activity and cytotoxic effects were analyzed by a correlation analysis.
  
  
  RESULTS
  Following the increase of the concentration and the time of CDDP, gradually repressed telomerase activity was detected in cultured cells. Meanwhile, the restrain rate of the cells increased. The telomerase activity at 24 h and 1 microgram/ml was repressed significantly compared with the control cells. However, the appearance of cell death lagged behind the decreasing of telomerase.
  
  
  CONCLUSIONS
  CDDP is an effective telomerase inhibitor in cultured choroidal melanoma cells of human eyes, which presents concentration and time dependency and can cause the death of cultured cells.
authors.affiliation: Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Cheng
authors.forename: Zhongyao
authors.initials: Z
authors.lastname: Wu
authors.forename: Jianliang
authors.initials: J
authors.lastname: Zheng
authors.forename: Guilan
authors.initials: G
authors.lastname: Lu
authors.forename: Jianhua
authors.initials: J
authors.lastname: Yan
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.forename: Danping
authors.initials: D
authors.lastname: Huang
authors.forename: Jianxian
authors.initials: J
authors.lastname: Lin
mesh_terms: D000328:Adult; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D016923:Cell Death / Q000187:drug effects; D002830:Choroid Neoplasms / Q000201:enzymology / Q000473:pathology*; D002945:Cisplatin / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008297:Male; D008545:Melanoma / Q000201:enzymology / Q000473:pathology*; D008875:Middle Aged; D019098:Telomerase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D019098:Telomerase; D002945:Cisplatin