The acute effects of acrylamide on astrocyte functions.
We assessed biochemical endpoints indicative of acute toxicity in neonatal rat primary astrocyte cultures exposed to acrylamide. Metallothionein (MT), glutamine synthetase (GS), glutamate/aspartate transporter (GLAST), and taurine transporter (tau-T) mRNA expression levels as well as cell volume were determined in astrocytes acutely treated with 0.1 and 1.0 mM acrylamide. Statistically significant changes in acrylamide treated astrocytes were noted for GS (0.1 mM) and GLAST (1.0 mM) mRNA expression levels. All other measurements were insignificant in comparison with controls, suggesting that astrocytic function is minimally compromised even at exceedingly high levels of acute acrylamide exposure.
Aschner
Michael
M
Department of Physiology and Pharmacology and Interdisciplinary Program in Neuroscience, Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157, USA. maschner@wfubmc.edu
Cao
Cheng-Chen
CC
Wu
Qi
Q
Friedman
Marvin A
MA
D020106:Acrylamide / Q000494:pharmacology* / Q000633:toxicity; D027322:Amino Acid Transport System X-AG / Q000235:genetics / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D048429:Cell Size; D002478:Cells, Cultured; D005974:Glutamate-Ammonia Ligase / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D026901:Membrane Transport Proteins; D008668:Metallothionein / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D000099247:Taurine Transporters
D020106:Acrylamide; D027322:Amino Acid Transport System X-AG; D002352:Carrier Proteins; D005974:Glutamate-Ammonia Ligase; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D008668:Metallothionein; D000099247:Taurine Transporters