Enhanced production of recombinant B-domain deleted factor VIII from Chinese hamster ovary cells by propionic and butyric acids.
Sodium propionate, as well as sodium butyrate, enhanced the production of recombinant B-domain-deleted, factor VIII (rFVIIIdB) by Chinese hamster ovary cells growing in a spinner-flask with a protein-free medium by more than six-fold. The two acids, however, had different cytotoxicities.
Chun
Bok-Hwan
BH
Department of Agricultural Chemistry, College of Life and Environmental Sciences, Korea University, 5 Ga Anamdong, Sungbook-gu, Seoul 136-075, Korea.
Park
Song-Yong
SY
Chung
Namhyun
N
Bang
Won-Gi
WG
D000818:Animals; D019149:Bioreactors; D020148:Butyric Acid / Q000494:pharmacology*; D016466:CHO Cells / Q000187:drug effects* / Q000378:metabolism* / Q000502:physiology; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D005169:Factor VIII / Q000096:biosynthesis* / Q000235:genetics; D010446:Peptide Fragments / Q000096:biosynthesis* / Q000235:genetics; D011422:Propionates / Q000494:pharmacology*; D011786:Quality Control; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D012680:Sensitivity and Specificity
C451034:B-domain-deleted factor VIII; D010446:Peptide Fragments; D011422:Propionates; D011994:Recombinant Proteins; D020148:Butyric Acid; D005169:Factor VIII; C514135:sodium propionate; C029658:propionic acid