title: Interaction of EBV latent origin of replication with the nuclear matrix: identification of S/MAR sequences and protein components.
abstract: During latency, Epstein Barr virus (EBV) genome, as an episome, is attached to the nuclear matrix (NM) via the latent origin of replication ori P. Within this element, we have found that a region, 580 bp long, encompassing the replicator DS element, shows the strongest affinity for the NM. In addition, by cross-linking with cis-diamminedichloroplatinum, we have identified two NM proteins with an apparent molecular weight of 85 and 60 kDa that, with high affinity and specificity, bind ori P. These proteins are not induced by EBV infection, but their interaction with ori P is lost upon induction of EBV lytic cycle. These data strongly suggest that the binding of ori P to specific components of the NM is required for EBV latent replication.
authors.affiliation: Department of Public Health Sciences, University La Sapienza, Rome, Italy. giulia.mearini@hpi.uni-hamburg.de <giulia.mearini@hpi.uni-hamburg.de>
authors.forename: Giulia
authors.initials: G
authors.lastname: Mearini
authors.forename: Silvia
authors.initials: S
authors.lastname: Chichiarelli
authors.forename: Michele
authors.initials: M
authors.lastname: Zampieri
authors.forename: Silvia
authors.initials: S
authors.lastname: Masciarelli
authors.forename: Maria
authors.initials: M
authors.lastname: D'Erme
authors.forename: Anna
authors.initials: A
authors.lastname: Ferraro
authors.forename: Elena
authors.initials: E
authors.lastname: Mattia
mesh_terms: D001665:Binding Sites; D002051:Burkitt Lymphoma; D002460:Cell Line; D017931:DNA Primers; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D015530:Nuclear Matrix / Q000821:virology*; D016133:Polymerase Chain Reaction; D018741:Replication Origin / Q000235:genetics*; D015183:Restriction Mapping
chemical_list: D017931:DNA Primers; D004279:DNA, Viral