Bovine monocytes induce immunoglobulin production in peripheral blood B lymphocytes.
Although a role for monocytes and monocyte-derived dendritic cells (DC) in the activation of T cells is well established, it is less clear to what extent DC and their precursors, monocytes, regulate B cell immune responses. Here we show that regulatory mechanisms similar to those in humans are in place in the bovine immune system. In vitro culture of bovine monocytes with bovine B cells activated by the anti-CD3 triggered CD4+ T cells or through immunoglobulin (Ig) receptor crosslinking induces B cell Ig secretion. Unlike bovine monocyte-derived DC, monocytes do not promote Ig class switching to IgG and IgA in activated peripheral blood B cells. These results suggest that bovine monocytes are capable of directly inducing Ig secretion in activated bovine peripheral blood B cells, but do not provide the signals for B cell Ig class switching.
Kruger
E F
EF
Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, P.O. Box 6100, Spring Street, Mississippi State, MS 39762-6100, USA.
Boyd
B L
BL
Pinchuk
L M
LM
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D017252:CD3 Complex / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D002417:Cattle; D002449:Cell Aggregation; D002478:Cells, Cultured; D018920:Coculture Techniques; D005434:Flow Cytometry; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology / Q000378:metabolism; D007075:Immunoglobulin M / Q000276:immunology / Q000378:metabolism; D007136:Immunoglobulins / Q000276:immunology / Q000378:metabolism*; D009000:Monocytes / Q000166:cytology / Q000378:metabolism*
D017252:CD3 Complex; D007070:Immunoglobulin A; D007075:Immunoglobulin M; D007136:Immunoglobulins