Oxygen supply modulates MCP-1 release in monocytes from young and aged rats: decrease of MCP-1 transcription and translation is age-related.
Hyper or hypoxia may affect the immune system's chemokine production. Monocyte chemotactic protein-1 (MCP-1), an important chemotactic cytokine can be activated by active oxygen species. Groups of rats were exposed to hypoxic and hyperoxic environmental conditions for 60 h and MCP-1 was determined in their peripheral blood mononuclear cells by Elisa and Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). In this study we evaluated if the ability of monocytes to produce MCP-1 under basal conditions or after stimulation with lipopolysaccharide (LPS) or phytohaemagglutinin (PHA) was differently affected by exposure to hyper or hypoxic conditions in young and aged rats. MCP-1 expression and production in monocyte/macrophages from rats at normoxic conditions was reduced in aged subjects. However, spontaneous, LPS or PHA-induced MCP-1 production was up-regulated by exposure to hyperoxic conditions in both young (62 +/- 8, 99 +/- 7, 102 +/- 8 pg/ml, respectively) and aged rats (79 +/- 4, 112 +/- 9, 117 +/- 10 pg/ ml, respectively). We conclude that hyperoxia is an important regulator of MCP-1 release and support the hypothesis that increased % of O2 may serve to initiate MCP-1 production which then serves to recruit and regulate the distribution of mononuclear cells to the sites of inflammation.
Reale
M
M
Immunology Division, Department of Oncology and Neurosciences, School of Medicine, University of Chieti, Chieti, Italy.
Di Giulio
C
C
Cacchio
M
M
Barbacane
R C
RC
Grilli
A
A
Felaco
M
M
Bianchi
G
G
Di Gioacchino
M
M
Conti
P
P
D000375:Aging; D000818:Animals; D018932:Chemokine CCL2 / Q000378:metabolism*; D000860:Hypoxia; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D009000:Monocytes / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D014176:Protein Biosynthesis; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014158:Transcription, Genetic
D018932:Chemokine CCL2; D010100:Oxygen