title: Changes of balance between proteinase and their inhibitors in blood of pigs with high-velocity missile wounds.
abstract: OBJECTIVE
  To study the effect of imbalance between lysosomal enzymes and their inhibitors in blood on disturbance of the local and whole body after trauma.
  
  
  METHODS
  The dynamic changes of lysosomal enzymes and proteinase inhibitors were studied in 12 pigs with femoral comminuted fractures in both hind limbs caused by high velocity missiles. Four normal pigs served as controls.
  
  
  RESULTS
  After injury, the activity of Cathepsin D in arterial plasma increased gradually and reached the highest level at 8 hours, acid phosphatase in serum began to increase at 12 hours and the value of serum elastase did not change significantly. The level of alpha1-antitrypsin, a proteinase inhibitor in plasma, decreased significantly in the early stage after injury [73.5%+/-6.4% and 81.0%+/-5.1% of the baseline value (1.67 micromol x ml(-1) x min(-1)+/- 0.29 micromol x ml(-1) x min(-1)) at l and 2 hours after injury, respectively, P<0.05], then increased gradually and was higher than the baseline value at 12 hours after injury.
  
  
  CONCLUSIONS
  Imbalance between lysosomal enzymes and proteinase inhibitors occurs soon after injury, which might result in continuous tissue damage and play an important role in the disturbance of general reaction after injury.
authors.affiliation: Molecular Biology Center, Research Institute of Surgery Daping Hospital, Third Military Medical University, Chongqing 400042, China. ygzhou@cta.cq.cn
authors.forename: Yuan-guo
authors.initials: YG
authors.lastname: Zhou
authors.forename: Pei-fang
authors.initials: PF
authors.lastname: Zhu
authors.forename: Ji-hong
authors.initials: JH
authors.lastname: Zhou
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Li
mesh_terms: D000135:Acid Phosphatase / Q000097:blood; D000818:Animals; D002402:Cathepsin D / Q000097:blood; D010450:Endopeptidases / Q000097:blood*; D005260:Female; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D010196:Pancreatic Elastase / Q000097:blood; D013552:Swine; D014948:Wounds, Gunshot / Q000097:blood*; D000515:alpha 1-Antitrypsin / Q000032:analysis*
chemical_list: D000515:alpha 1-Antitrypsin; D000135:Acid Phosphatase; D010450:Endopeptidases; D010196:Pancreatic Elastase; D002402:Cathepsin D