title: The AmCREB gene is an ortholog of the mammalian CREB/CREM family of transcription factors and encodes several splice variants in the honeybee brain.
abstract: The transcription factor CREB (cAMP response element binding protein) is required for the switch from short-term to long-term synaptic plasticity and from short-term to long-term memory. Its activity is regulated by the cAMP-dependent signalling cascade, which has been shown to play a crucial role in the honeybee's long-term memory formation. To elucidate the role of the CREB in honeybee memory formation we analysed a CREB-homologous gene, AmCREB, which is expressed as several transcripts in the honeybee brain. Eight transcripts have been identified (AmCREB 1-8) that are generated by alternate splicing. One antibody generated against a subset of these variants reveals a cytosolic localization in the mushroom body alpha-lobes, the glomeruli of the antennal lobes, the protocerebral lobes, the central complex and in the optical lobes.
authors.affiliation: Institut für Biologie-Neurobiologie, Freie Universität Berlin, Germany. theodora@zedat.fu-berlin.de
authors.forename: D
authors.initials: D
authors.lastname: Eisenhardt
authors.forename: A
authors.initials: A
authors.lastname: Friedrich
authors.forename: N
authors.initials: N
authors.lastname: Stollhoff
authors.forename: U
authors.initials: U
authors.lastname: Müller
authors.forename: H
authors.initials: H
authors.lastname: Kress
authors.forename: R
authors.initials: R
authors.lastname: Menzel
mesh_terms: D017398:Alternative Splicing / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001516:Bees / Q000235:genetics* / Q000378:metabolism; D015152:Blotting, Northern; D001921:Brain / Q000378:metabolism* / Q000502:physiology; D017362:Cyclic AMP Response Element-Binding Protein / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D014644:Genetic Variation / Q000502:physiology; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012313:RNA / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment
chemical_list: D017362:Cyclic AMP Response Element-Binding Protein; D012313:RNA