title: Single-scattering spectroscopy for the endoscopic analysis of particle size in superficial layers of turbid media.
abstract: We report on the development of an optical-fiber-based diagnostic tool that is sensitive to single-scattering events close to the fiber-optic probe tip. By using a single fiber to deliver and detect white light we optimised the detection probability of singly scattered photons from small depths. The sampling depth of this delivery-and-collection fiber was investigated by use of a tissue phantom. We found that for our phantom 90% of the single-scattering signal in the delivery-and-collection fiber originated from less than 200 microm from the fiber tip. The contribution of multiply scattered light from a greater depth to the signal was measured with an additional collection fiber. Several tissue phantoms demonstrated our fiber-optic probes sensitivity to light scattering from superficial layers of tissue and thereby its potential to detect superficial precancerous epithelial lesions.
authors.affiliation: Erasmus MC, P.O. Box 5201, 3008 AE Rotterdam, The Netherlands. amelink@kfih.azr.nl
authors.forename: Arjen
authors.initials: A
authors.lastname: Amelink
authors.forename: Martin P L
authors.initials: MP
authors.lastname: Bard
authors.forename: Sjaak A
authors.initials: SA
authors.lastname: Burgers
authors.forename: Henricus J C M
authors.initials: HJ
authors.lastname: Sterenborg
mesh_terms: D016880:Anisotropy; D017076:Computer-Aided Design; D004724:Endoscopy / Q000379:methods*; D004848:Epithelium / Q000502:physiology; D005336:Fiber Optic Technology / Q000295:instrumentation* / Q000379:methods*; D009391:Nephelometry and Turbidimetry / Q000295:instrumentation* / Q000379:methods*; D055100:Optical Fibers; D010316:Particle Size; D019047:Phantoms, Imaging; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000295:instrumentation* / Q000379:methods*; D013499:Surface Properties