The zebrafish spi1 promoter drives myeloid-specific expression in stable transgenic fish.
The spi1 (pu.1) gene has recently been identified as a useful marker of early myeloid cells in zebrafish. To enhance the versatility of this organism as a model for studying myeloid development, the promoter of this gene has been isolated and characterized. Transient transgenesis revealed that a 5.3 kilobase promoter fragment immediately upstream of the spi1 coding sequence was sufficient to drive expression of enhanced green fluorescent protein (EGFP) in injected embryos in a manner that largely recapitulated the native spi1 gene expression pattern. This fragment was successfully used to produce a germ line transgenic line of zebrafish with EGFP-expressing myeloid cells. These TG(spi1:EGFP)pA301 transgenic zebrafish represent a valuable tool for further studies of myeloid development and its perturbation.
Ward
Alister C
AC
Centre for Cellular and Molecular Biology, School of Biological and Chemical Sciences, Deakin University, Burwood, Victoria, Australia. award@deakin.edu.au
McPhee
Dora O
DO
Condron
Melanie M
MM
Varma
Sony
S
Cody
Stephen H
SH
Onnebo
Sara M N
SM
Paw
Barry H
BH
Zon
Leonard I
LI
Lieschke
Graham J
GJ
D000818:Animals; D030801:Animals, Genetically Modified; D018076:DNA, Complementary; D004625:Embryo, Nonmammalian; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics; D022423:Myeloid Cells / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D015534:Trans-Activators / Q000235:genetics*; D019076:Transgenes; D015027:Zebrafish / Q000235:genetics*; D000099232:Proto-Oncogene Protein Spi-1
D018076:DNA, Complementary; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D011518:Proto-Oncogene Proteins; D015534:Trans-Activators; D000099232:Proto-Oncogene Protein Spi-1