Grape seeds: the best lignocellulosic waste to produce laccase by solid state cultures of Trametes hirsuta.
Grape seeds were used by Trametes hirsuta as a substrate for laccase production giving 23 kU l(-1), which was 10-fold the value attained in the cultures with no lignocellulosic waste addition. The dyes, Indigo Carmine and Bromophenol Blue, were easily decolourised (100% in 24 h) by the extracellular liquid obtained in such cultures, whereas Methyl Orange (65% in 24 h) and Phenol Red (36% in 24 h) were more resistant to degradation. This shows the specificity of laccase towards different dye structures.
Moldes
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Department of Chemical Engineering, University of Vigo, 36200 Vigo, Spain.
Gallego
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Rodríguez Couto
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Sanromán
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D018929:Cell Culture Techniques / Q000379:methods*; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism*; D003116:Color; D004396:Coloring Agents / Q000737:chemistry; D019327:Copper Sulfate / Q000494:pharmacology; D001467:Hordeum / Q000737:chemistry; D042845:Laccase; D008031:Lignin / Q000737:chemistry / Q000378:metabolism*; D010088:Oxidoreductases / Q000096:biosynthesis*; D020072:Polyporales / Q000187:drug effects / Q000378:metabolism*; D011786:Quality Control; D012037:Refuse Disposal / Q000379:methods; D012639:Seeds / Q000737:chemistry / Q000378:metabolism; D013379:Substrate Specificity; D014815:Vitamins / Q000494:pharmacology; D027843:Vitis / Q000737:chemistry* / Q000378:metabolism
D004396:Coloring Agents; D014815:Vitamins; C036909:lignocellulose; D002482:Cellulose; D008031:Lignin; D010088:Oxidoreductases; D042845:Laccase; D019327:Copper Sulfate