title: Role for plastin in host defense distinguishes integrin signaling from cell adhesion and spreading.
abstract: Integrin ligation activates both cell adhesion and signal transduction, in part through reorganization of the actin cytoskeleton. Plastins (also known as fimbrins) are actin-crosslinking proteins of the cortical cytoskeleton present in all cells and conserved from yeast to mammals. Here we show that plastin-deficient polymorphonuclear neutrophils (PMN) are deficient in killing the bacterial pathogen Staphylococcus aureus in vivo and in vitro, despite normal phagocytosis. Like integrin beta2-deficient PMN, plastin-deficient PMN cannot generate an adhesion-dependent respiratory burst, because of markedly diminished integrin-dependent syk activation. Unlike beta2(-/-) PMN, plastin-deficient PMN adhere and spread normally. Deficiency of plastin thus separates the classical integrin receptor functions of adhesion and spreading from intracellular signal transduction.
authors.affiliation: Program in Microbial Pathogenesis and Host Defense and University of California, San Francisco, San Francisco, CA 94143, USA.
authors.forename: Hua
authors.initials: H
authors.lastname: Chen
authors.forename: Attila
authors.initials: A
authors.lastname: Mocsai
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Rong-Xian
authors.initials: RX
authors.lastname: Ding
authors.forename: J Hiroshi
authors.initials: JH
authors.lastname: Morisaki
authors.forename: Michael
authors.initials: M
authors.lastname: White
authors.forename: Jacob M
authors.initials: JM
authors.lastname: Rothfork
authors.forename: Patrick
authors.initials: P
authors.lastname: Heiser
authors.forename: Emma
authors.initials: E
authors.lastname: Colucci-Guyon
authors.forename: Clifford A
authors.initials: CA
authors.lastname: Lowell
authors.forename: Hattie D
authors.initials: HD
authors.lastname: Gresham
authors.forename: Paul M
authors.initials: PM
authors.lastname: Allen
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Brown
mesh_terms: D000818:Animals; D018821:CD18 Antigens / Q000502:physiology*; D002448:Cell Adhesion; D002465:Cell Movement; D004792:Enzyme Precursors / Q000502:physiology; D007113:Immunity, Innate; D047908:Intracellular Signaling Peptides and Proteins; D008562:Membrane Glycoproteins; D051379:Mice; D008810:Mice, Inbred C57BL; D008840:Microfilament Proteins; D009504:Neutrophils / Q000276:immunology* / Q000502:physiology; D010750:Phosphoproteins / Q000502:physiology*; D011505:Protein-Tyrosine Kinases / Q000502:physiology; D016897:Respiratory Burst; D015398:Signal Transduction / Q000502:physiology*; D013203:Staphylococcal Infections / Q000276:immunology; D000072377:Syk Kinase
chemical_list: D018821:CD18 Antigens; D004792:Enzyme Precursors; D047908:Intracellular Signaling Peptides and Proteins; D008562:Membrane Glycoproteins; D008840:Microfilament Proteins; D010750:Phosphoproteins; C026742:plastin; D011505:Protein-Tyrosine Kinases; D000072377:Syk Kinase; C000606897:Syk protein, mouse