Evaluation of human beta-enolase as a serum marker for exercise-induced muscle damage.
OBJECTIVE
To evaluate whether the serum beta-enolase level is a useful indicator of exercise-induced muscle damage in athletes.


METHODS
Blood samples were taken from 49 adult amateur marathon runners before and immediately after a marathon race, and the serum levels of beta-enolase and creatine phosphokinase were measured.


METHODS
The Aoshima Taiheiyo Marathon 2000, Miyazaki, Japan, on a cloudy day in December with an ambient temperature of 18 degrees C.


METHODS
Forty-nine adult amateur marathon runners (42 men and 7 women) who regularly participated in runs.


METHODS
The intervention was a marathon run.


METHODS
Serum beta-enolase was measured using a sensitive sandwich enzyme-linked immunosorbent assay. Serum creatine phosphokinase was measured using a standard procedure.


RESULTS
The mean beta-enolase concentration was 9.45 +/- 3.11 ng/mL before the race. It increased to 22.11 +/- 8.80 ng/mL after the race, representing a proportional increase of 1.57 +/- 1.46. The serum concentration of beta-enolase after the race was significantly higher than that before the race (P < 0.0001). Moreover, the serum beta-enolase level increased as much as the creatine phosphokinase level after the race, and strongly correlated with creatine phosphokinase (r = 0.828, P < 0.0001). The proportional increase of beta-enolase also correlated with that of creatine phosphokinase (r = 0.441, P < 0.005).


CONCLUSIONS
Our data suggest that the absolute values of the serum beta-enolase are more appropriate to relate to muscle damage.
Chosa
Etsuo
E
Department of Orthopaedic Surgery, Miyazaki Medical College, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan. chosa@post.miyazaki-med.ac.jp
Sekimoto
Tomohisa
T
Sonoda
Norio
N
Yamamoto
Keitaro
K
Matsuda
Hirokazu
H
Takahama
Keiichi
K
Tajima
Naoya
N
D000328:Adult; D015415:Biomarkers / Q000097:blood; D015331:Cohort Studies; D003402:Creatine Kinase / Q000097:blood* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D015444:Exercise; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000201:enzymology* / Q000293:injuries*; D009135:Muscular Diseases / Q000201:enzymology / Q000503:physiopathology; D010751:Phosphopyruvate Hydratase / Q000097:blood* / Q000378:metabolism; D011336:Probability; D011379:Prognosis; D012420:Running / Q000293:injuries; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index
D015415:Biomarkers; D003402:Creatine Kinase; D010751:Phosphopyruvate Hydratase