Analysis of altered gene expression profiles in retinoic acid or CpG-treated Sprague-Dawley rats with MNU-induced mammary adenocarcinoma by cDNA macro array.
In the present work the effects of 13-cis retinoic acid (RA) and CpG-containing oligodeoxynucleotides (CpG-ODN) on the gene expression profile of spleen and tumor tissue in a MNU-induced mammary gland carcinoma ratmodel were investigated by the use of a commercial cDNA macro array (Atlas rat toxicology array 1.2, Clontech). Treatment with these components, either alone or in combination, induced differences of the expression profiles between the distinct treatment groups in both tissues. The large number of genes with altered expression (> 200) points to a highly complex process in vivo.
Mostböck
S
S
Institute of Experimental Endocrinology, Slovak Academy of Science, Bratislava, Slovakia.
Macejová
D
D
Baranová
M
M
Weiss
R
R
Scheiblhofer
S
S
Verwanger
T
T
Krammer
B
B
Thalhamer
J
J
Brtko
J
J
D000230:Adenocarcinoma / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism; D000818:Animals; D018899:CpG Islands; D004252:DNA Mutational Analysis / Q000379:methods; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects* / Q000235:genetics; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000235:genetics* / Q000378:metabolism; D008770:Methylnitrosourea; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000187:drug effects; D014212:Tretinoin / Q000494:pharmacology*
D009838:Oligodeoxyribonucleotides; D014212:Tretinoin; D008770:Methylnitrosourea