EI-1941-1 and -2, novel interleukin-1beta converting enzyme inhibitors produced by Farrowia sp. E-1941. I. Biochemical characterization of EI-1941-1 and -2.
EI-1941-1 and -2 isolated from the culture broths of Farrowia sp. selectively inhibited the human recombinant ICE activity with IC50 values of 0.086 and 0.006 microM, respectively, without inhibiting elastase and cathepsin B. EI-1941-1 and -2 also inhibited mature interleukin-1beta secretion from THP-1 cells induced by LPS with IC50 values of 5.0 and 10.3 microM, respectively. Biochemical characterizations of EI-1941-1 and -2 are described in this article.
Koizumi
Fumito
F
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahimachi, Machida-shi, Tokyo 194-8533, Japan. fumito.koizumi@kyowa.co.jp
Matsuda
Yuzuru
Y
Nakanishi
Satoshi
S
D003512:Cyclohexanones / Q000737:chemistry / Q000494:pharmacology*; D003545:Cysteine / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004355:Drug Stability; D004852:Epoxy Compounds / Q000737:chemistry / Q000494:pharmacology*; D005978:Glutathione / Q000494:pharmacology; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism; D015843:Serpins / Q000737:chemistry / Q000494:pharmacology*; D014764:Viral Proteins
D003512:Cyclohexanones; C476113:EI-1941-1; C476114:EI-1941-2; D004852:Epoxy Compounds; D007375:Interleukin-1; D015843:Serpins; D014764:Viral Proteins; C074641:interleukin-1beta-converting enzyme inhibitor; D005978:Glutathione; D003545:Cysteine; D004229:Dithiothreitol