Group I mGluR ligands fail to affect 6-hydroxydopamine- induced death and glutamate release of PC12 cells.
OBJECTIVE
To study the effect of group I metabotropic glutamate receptor (mGluR) ligands on 6-hydroxydopamine (6-OHDA)-induced death and glutamate release of PC12 cells.


METHODS
PC12 cells were exposed to 100 micromol/L of group I mGluR agonist (RS)-3,5-dihydroxy-phenylglycine (DHPG) or antagonist DL-2-amino-3-phosphonopropionic acid (DL-AP3) 1 h before addition of 6-OHDA 100 micromol/L. After incubation for 24 h, morphological alterations were observed with microscope, DNA fragmentation was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) method, cytotoxicity was measured by MTT assay, and glutamate release was assayed by high performance liquid chromatography.


RESULTS
6-OHDA decreased cell viability (P<0.01) and induced concentration- and time-dependent glutamate release from PC12 cells. Group I mGluR ligands did not affect 6-OHDA-induced death of PC12 cells and had no influence on glutamate levels.


CONCLUSIONS
Group I mGluR ligands cannot protect PC12 cells from 6-OHDA-induced death.
Li
Hao
H
Department of Pharmacology and Neurobiology, Nanjing Medical University, Nanjing 210029, China.
Ding
Jian-Hua
JH
Hu
Gang
G
D018663:Adrenergic Agents / Q000494:pharmacology; D000409:Alanine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D017209:Apoptosis; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation; D004347:Drug Interactions; D018698:Glutamic Acid / Q000378:metabolism*; D008024:Ligands; D008734:Methoxyhydroxyphenylglycol / Q000031:analogs & derivatives* / Q000494:pharmacology*; D016627:Oxidopamine / Q000494:pharmacology*; D016716:PC12 Cells; D051381:Rats; D018094:Receptors, Metabotropic Glutamate / Q000819:agonists* / Q000037:antagonists & inhibitors
D018663:Adrenergic Agents; D008024:Ligands; D018094:Receptors, Metabotropic Glutamate; C000211:2-amino-3-phosphonopropionic acid; D018698:Glutamic Acid; D008734:Methoxyhydroxyphenylglycol; D016627:Oxidopamine; D000409:Alanine; C010117:3,4-dihydroxyphenylglycol