Running interference for hedgehog signaling.
The new technology of RNA interference (RNAi) is allowing high-throughput screening of cells to examine many biological processes. Michelson discusses one such system in which the Drosophila Hedgehog (Hh) pathway was analyzed by systematic genome-wide RNAi analysis. The results not only suggest unexpected overlap in the Hh and Wingless (Wg) pathways, but also provide proof of principle for the development of high-throughput assays using RNAi techniques.
Michelson
Alan M
AM
Division of Genetics, Department of Medicine, Howard Hughes Medical Institute, Brigham and Women's Hospital, 20 Shattuck Street, Boston, MA 02115, USA. michelson@rascal.med.harvard.edu
D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics / Q000502:physiology*; D053823:Hedgehog Proteins; D006801:Humans; D034622:RNA Interference / Q000502:physiology*; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology*
D029721:Drosophila Proteins; D053823:Hedgehog Proteins; C077245:hh protein, Drosophila