title: Poly(hydroxybutyrate-co-hydroxyhexanoate) promoted production of extracellular matrix of articular cartilage chondrocytes in vitro.
abstract: The present investigation describes the production of extracellular matrix of rabbit articular cartilage chondrocytes grown on scaffolds of polyhydroxybutyrate (PHB) blended with poly(hydroxybutyrate-co-hydroxyhexanoate) (PHBHHx) for up to 7 days. The mRNA level of type II collagen of chondrocytes seeded on all scaffolds consisting of PHBHHx were obviously higher than that of PHB-only scaffold throughout the culture period, suggesting the positive effect of PHBHHx on extracellular matrix production. Second-harmonic generation (SHG) imaging technique, combined with confocal fluorescence microscopy (CFM) revealed that PHBHHx in PHB scaffold provided better surface properties for anchoring type II collagen filaments and their penetration into internal layers of the scaffolds. Glycosaminoglycan (GAG), a major composition of extracellular matrix, showed a sharp increase in construct of 1:2 PHB/PHBHHx scaffold after 7 day cultivation, while only a small increase was observed in all other tested scaffolds. At the same time, total collagen contents in all scaffolds containing PHBHHx increased with time, with the maximum collagen production of 742.1+/-99.2mg/g dry weight observed in construct of 1:2 PHB/PHBHHx scaffold inoculated for 7 days, this was almost 4-fold higher than that in scaffold of PHB only. It appears that the presence of right proportion of PHBHHx in the composite system of PHB/PHBHHx highly favored the production of extracellular matrix of articular cartilage chondrocytes.
authors.affiliation: Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Deng
authors.forename: Xing-sun
authors.initials: XS
authors.lastname: Lin
authors.forename: Zhong
authors.initials: Z
authors.lastname: Zheng
authors.forename: Jin-Guang
authors.initials: JG
authors.lastname: Deng
authors.forename: Jin-Chun
authors.initials: JC
authors.lastname: Chen
authors.forename: Hui
authors.initials: H
authors.lastname: Ma
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Chen
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000737:chemistry* / Q000494:pharmacology; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002208:Caproates / Q000737:chemistry* / Q000494:pharmacology; D002356:Cartilage / Q000378:metabolism; D002358:Cartilage, Articular / Q000737:chemistry / Q000378:metabolism*; D019902:Chondrocytes / Q000378:metabolism*; D003094:Collagen / Q000737:chemistry; D024043:Collagen Type II / Q000737:chemistry; D005109:Extracellular Matrix / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000737:chemistry; D006885:Hydroxybutyrates / Q000737:chemistry; D007091:Image Processing, Computer-Assisted; D066298:In Vitro Techniques; D018613:Microscopy, Confocal; D011091:Polyesters / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D000094122:Polyhydroxybutyrates
chemical_list: D020155:3-Hydroxybutyric Acid; D001672:Biocompatible Materials; D002208:Caproates; D003094:Collagen; D024043:Collagen Type II; D006025:Glycosaminoglycans; D006885:Hydroxybutyrates; D011091:Polyesters; D011108:Polymers; D012333:RNA, Messenger; C115940:poly(3-hydroxybutyrate-co-3-hydroxyhexanoate); D000094122:Polyhydroxybutyrates; C003182:poly-beta-hydroxybutyrate