Self-assembling monolayer formation of glucose oxidase covalently attached on 11-aminoundecanethiol monolayers on gold.
Glucose oxidase (GOx) has been attached covalently to form uniform enzyme monolayers on self-assembled monolayers (SAMs) from 11-aminoundecanethiol (AUDT) by taking advantage of chemical oxidation of GOx carbohydrate residues followed by coupling the resulting 'aldehydic' enzyme with the terminal amino group in the SAM as characterized by AFM imaging, IR, QCM, and electrochemical measurements.
Nakano
Koji
K
Department of Applied Chemistry, Faculty of Engineering, Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan. kojitcm@mbox.nc.kyushu-u.ac.jp
Doi
Kenji
K
Tamura
Kousuke
K
Katsumi
Yosuke
Y
Tazaki
Masato
M
D000473:Alkanes / Q000032:analysis / Q000737:chemistry*; D005949:Glucose Oxidase / Q000032:analysis / Q000737:chemistry*; D006046:Gold / Q000032:analysis / Q000737:chemistry*; D008567:Membranes, Artificial
D000473:Alkanes; D008567:Membranes, Artificial; D006046:Gold; D005949:Glucose Oxidase; C022884:undecane