Electron microscopic localization of Nogo-A at the postsynaptic active zone of the rat.
Our previous study has demonstrated intraneuronal localization of Nogo-A in motoneurons of the adult rat spinal cord. In the present study, we wished to explore Nogo-A immunoreactivity at synapses using pre-embedding and post-embedding immunogold cytochemistry. Immunogold particles for Nogo-A could be identified in dendrites of the spinal cord motoneurons. Some Nogo-A immunogold particles were found in close apposition to the postsynaptic density and the postsynaptic dense bodies at both symmetric and asymmetric synapses. The present finding of Nogo-A at the postsynaptic active zone provides a morphological basis for the possibility that the Nogo-A-Nogo receptor system may contribute to structural plasticity at synapses as well as along the axonal pathway.
Liu
Ying Ying
YY
Institute of Neurosciences, The Fourth Military Medical University, 17 Chang Le Xi Road, Xi'an, 710032, PR China.
Jin
Wei Lin
WL
Liu
Hui Ling
HL
Ju
Gong
G
D000818:Animals; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D009046:Motor Neurons / Q000378:metabolism / Q000648:ultrastructure; D009185:Myelin Proteins / Q000378:metabolism*; D000070798:Nogo Proteins; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000378:metabolism / Q000648:ultrastructure; D013569:Synapses / Q000378:metabolism*
D009185:Myelin Proteins; D000070798:Nogo Proteins; C000603197:Rtn4 protein, rat