title: DNA topoisomerases I and II inhibitory activity of constituents isolated from Juglans mandshurica.
abstract: Nine diarylheptanoids (1-9), one triterpene (10), one sesquiterpenoid (11), one naphthoquinone (12), four tetralones (13-16), one naphthalene carboxylic acid glucoside (17) and six naphthalenyl glycosides (18-23) were isolated from the roots of Juglans mandshurica Maximowicz (Juglandaceae), and their structures determined from the chemical and spectral data. Here, we report the inhibitory effects, on the DNA topoisomerases I and II activities, of all these compounds. Compounds 10 and 23 showed more potent inhibitory effects, on the DNA topoisomerases I and II (94.0 and 86.0% inhibitions at the concentration of 5 microg/mL, respectively), than the positive control compounds, camptothecin and etoposide.
authors.affiliation: College of Pharmacy, Yeungnam University, Gyongsan 712-749, Korea.
authors.forename: Gao
authors.initials: G
authors.lastname: Li
authors.forename: Sun-Young
authors.initials: SY
authors.lastname: Lee
authors.forename: Kyeung-Seon
authors.initials: KS
authors.lastname: Lee
authors.forename: Sung-Won
authors.initials: SW
authors.lastname: Lee
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Seung-Ho
authors.initials: SH
authors.lastname: Lee
authors.forename: Chong-Soon
authors.initials: CS
authors.lastname: Lee
authors.forename: Mi-Hee
authors.initials: MH
authors.lastname: Woo
authors.forename: Jong-Keun
authors.initials: JK
authors.lastname: Son
mesh_terms: D000818:Animals; D002417:Cattle; D045744:Cell Line, Tumor / Q000187:drug effects / Q000201:enzymology; D004264:DNA Topoisomerases, Type I / Q000378:metabolism; D004250:DNA Topoisomerases, Type II / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D031324:Juglans; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018517:Plant Roots; D059004:Topoisomerase I Inhibitors; D059005:Topoisomerase II Inhibitors
chemical_list: D004791:Enzyme Inhibitors; D010936:Plant Extracts; D059004:Topoisomerase I Inhibitors; D059005:Topoisomerase II Inhibitors; D004264:DNA Topoisomerases, Type I; D004250:DNA Topoisomerases, Type II