[Construction of human papilloma virus type 18 E6E7 genes in adeno-associated virus expression vector and checking its activity for malignant transformation].
BACKGROUND
To construct human papillomavirus type 18 (HPV18 E6E7) adeno-associated virus (AAV) for studying the role of HPV E6E7 in the development of human cancer.


METHODS
HPV18 E6E7 genes were inserted into adeno-associated virus expression vector and then infected 293 cell line. The expression of HPV18 E6E7 genes were confirmed by using RT-PCR/Southern blot assay.


RESULTS
There was HPV18 E6E7 genes in the malignantly transformed cell line. The 293TL cells compared with the parent cells transformed cells grew more rapidly, lost their contact inhibition and formed more and large colonies in soft agar.


CONCLUSIONS
HPV18 E6E7 AAV was successfully constructed and could induce malignant transformation. HPV18 E6E7 AAV can be use for studying the immortalization and malignant transformation of human normal epithelial cells.
Cen
Shan
S
Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China.
Teng
Zhi-ping
ZP
Zhang
Yue
Y
Shen
Zhong-ying
ZY
Xu
Jin-jie
JJ
Du
Bin
B
Zeng
Yi
Y
D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D004279:DNA, Viral / Q000032:analysis; D004268:DNA-Binding Proteins; D000229:Dependovirus / Q000235:genetics*; D004847:Epithelial Cells / Q000166:cytology* / Q000821:virology; D005333:Fetus; D006801:Humans; D007668:Kidney / Q000166:cytology; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D027383:Papillomaviridae / Q000235:genetics; D016133:Polymerase Chain Reaction
D004279:DNA, Viral; D004268:DNA-Binding Proteins; C052603:E6 protein, Human papillomavirus type 18; C052633:E7 protein, Human papillomavirus type 18; D009856:Oncogene Proteins, Viral