Baculovirus induces an innate immune response and confers protection from lethal influenza virus infection in mice.
A recombinant baculovirus expressing the hemagglutinin gene of the influenza virus, A/PR/8/34 (H1N1), under the control of the chicken beta-actin promoter, was constructed. To determine the induction of protective immunity in vivo, mice were inoculated with the recombinant baculovirus by intramuscular, intradermal, i.p., and intranasal routes and then were challenged with a lethal dose of the influenza virus. Intramuscular or i.p. immunization with the recombinant baculovirus elicited higher titers of antihemagglutinin Ab than intradermal or intranasal administration. However, protection from a lethal challenge of the influenza virus was only achieved by intranasal immunization of the recombinant baculovirus. Surprisingly, sufficient protection from the lethal influenza challenge was also observed in mice inoculated intranasally with a wild-type baculovirus, as evaluated by reductions in the virus titer, inflammatory cytokine production, and pulmonary consolidations. These results indicate that intranasal inoculation with a wild-type baculovirus induces a strong innate immune response, which protects mice from a lethal challenge of influenza virus.
Abe
Takayuki
T
Department of Industrial Chemistry and High Technology Research Center, Chiba Institute of Technology, Tsudanuma, Narashino, Chiba, Japan. takaku@ic-it-chiba.ac.jp
Takahashi
Hitoshi
H
Hamazaki
Hiroyuki
H
Miyano-Kurosaki
Naoko
N
Matsuura
Yoshiharu
Y
Takaku
Hiroshi
H
D000281:Administration, Intranasal; D000818:Animals; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis; D003850:Deoxyribonuclease I / Q000737:chemistry; D004285:Dogs; D005260:Female; D005822:Genetic Vectors; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D006358:Hot Temperature; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics; D018836:Inflammation Mediators / Q000378:metabolism; D009980:Influenza A virus / Q000235:genetics / Q000276:immunology; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D007251:Influenza, Human / Q000276:immunology* / Q000401:mortality / Q000473:pathology / Q000517:prevention & control*; D007273:Injections, Intramuscular; D008168:Lung / Q000276:immunology / Q000473:pathology / Q000821:virology; D008262:Macrophage Activation / Q000235:genetics; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000276:immunology*; D012259:Ribonuclease, Pancreatic / Q000737:chemistry; D014407:Tumor Cells, Cultured; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
D016207:Cytokines; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D018836:Inflammation Mediators; D007252:Influenza Vaccines; D014614:Vaccines, Synthetic; D003850:Deoxyribonuclease I; D012259:Ribonuclease, Pancreatic