Transforming growth factor beta1 gene polymorphism in patients with systemic sclerosis.
OBJECTIVE
To determine whether transforming growth factor beta1 (TGFbeta1) gene DNA polymorphism is associated with pathogenesis in the fibrosis of patients with systemic sclerosis (SSc).


METHODS
Eighty-seven Japanese patients with SSc including 30 with diffuse type and 57 with limited type together with 110 unrelated controls were investigated. Pulmonary fibrosis was determined in 34 SSc patients using high-resolution chest computed tomography. TGFbeta1 genetic polymorphisms were analyzed in 2 loci; T869C (Leu10Pro) in codon 10 at exon 1, and C-509T in the promoter region using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).


RESULTS
Neither the genotype of T/C polymorphism in T869C nor C/T polymorphism in C-509T revealed any difference in distribution between SSc and controls. In the group of SSc patients with pulmonary fibrosis, a weak but significantly high frequency (p = 0.05) of TC+CC (the presence of C allele) in T869C, and CT+TT (the presence of T allele) in C-509T was found. Compared with controls, the pulmonary fibrosis group showed no difference in the highly frequent alleles.


CONCLUSIONS
Our results suggest that TGFbeta1 polymorphisms do not play a role in the pathogenesis of SSc, even though there remains the possibility of a risk factor for genetic susceptibility to pulmonary fibrosis.
Sugiura
Yoshiki
Y
Department of Internal Medicine and Molecular Science, Nagoya City University Graduate School of Medical Science, Nagoya-city, Aichi, Japan.
Banno
Shogo
S
Matsumoto
Yoshifuji
Y
Niimi
Takashi
T
Yoshinouchi
Takeo
T
Hayami
Yoshihito
Y
Naniwa
Taio
T
Ueda
Ryuzo
R
D000328:Adult; D004247:DNA / Q000097:blood; D016172:DNA Fingerprinting; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011658:Pulmonary Fibrosis / Q000209:etiology / Q000235:genetics* / Q000378:metabolism; D012595:Scleroderma, Systemic / Q000150:complications / Q000235:genetics* / Q000378:metabolism; D015291:Transforming Growth Factors / Q000235:genetics* / Q000378:metabolism
D015291:Transforming Growth Factors; D004247:DNA