The high-mobility-group box protein SSRP1/T160 is essential for cell viability in day 3.5 mouse embryos.
The high-mobility-group (HMG) SSRP1 protein is a member of a conserved chromatin-remodeling complex (FACT/DUF/CP) implicated in DNA replication, basal and regulated transcription, and DNA repair. To assist in the functional analysis of SSRP1, the Ssrp1 gene was targeted in murine embryonic stem cells, and the mutation was introduced into the germ line. Embryos homozygous for the targeted allele die soon after implantation, and preimplantation blastocysts are defective for cell outgrowth and/or survival in vitro. The Ssrp1 mutation was also crossed into a p53 null background without affecting growth and/or survival defects caused by loss of Ssrp1 function. Thus, Ssrp1 appears to encode nonredundant and p53-independent functions that are essential for cell viability.
Cao
Shang
S
Department of Microbiology and Immunology, Vanderbilt University School of Medicine, 1161 21st Avenue South, AA 4210 MCN, Nashville, TN 37232-2363, USA.
Bendall
Heather
H
Hicks
Geoffrey G
GG
Nashabi
Abudi
A
Sakano
Hitoshi
H
Shinkai
Yoichi
Y
Gariglio
Marisa
M
Oltz
Eugene M
EM
Ruley
H Earl
HE
D000483:Alleles; D000818:Animals; D017209:Apoptosis; D015139:Blotting, Southern; D015153:Blotting, Western; D002455:Cell Division; D002470:Cell Survival; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000502:physiology*; D016158:Genes, p53; D005822:Genetic Vectors; D005838:Genotype; D006609:High Mobility Group Proteins / Q000737:chemistry / Q000502:physiology*; D006720:Homozygote; D020287:In Situ Nick-End Labeling; D051379:Mice; D008957:Models, Genetic; D009154:Mutation; D011995:Recombination, Genetic; D013234:Stem Cells / Q000378:metabolism; D013997:Time Factors
D004268:DNA-Binding Proteins; D006609:High Mobility Group Proteins; C070454:Ssrp1 protein, mouse