Congenital lack of COX-2 affects mechanical and geometric properties of bone in mice.
We compared the mechanical properties of bones from mice lacking either a functional cycloxygenase-1 (C57BL6/DBA COX-1-/-; n = 9) or COX-2 (C57BL6/DBA COX-2-/-; n = 9) gene and wild type mice (C57BL6/DBA; n = 10). Twenty-eight right femora from 3-month-old male mice were used to determine bulk structural and material properties of bone by three-point bending. Bone matrix properties were also measured by nanoindentation to access the changes in bulk mechanical properties due to changes in bone matrix or bone geometry. The bulk material properties (elastic modulus, P < 0.05; ultimate stress, P < 0.01) of COX-2-/- bones were lower than those of wild-type mice whereas the bulk structural properties (stiffness, P > 0.2; breaking force, P > 0.1) were similar to those of the wild-type mice. COX-2-/- mice had a longer moment of inertia but their cortical bones were thinner and contained many more intra-cortical pores compared with the bones of the other two groups. Finally, the bone matrix properties of COX-1-/- mice, COX-2-/- mice and their heterozygous littermates were similar to those of C57BL6/DBA wild-type mice.
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Department of Biomedical Engineering, University of Memphis, Memphis, Tennessee, USA.
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Laulederkind
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D000818:Animals; D001696:Biomechanical Phenomena; D001847:Bone Diseases / Q000151:congenital / Q000201:enzymology / Q000235:genetics; D001857:Bone Matrix / Q000473:pathology / Q000503:physiopathology*; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D004247:DNA / Q000032:analysis; D005269:Femur / Q000473:pathology / Q000503:physiopathology*; D005838:Genotype; D007527:Isoenzymes / Q000235:genetics*; D008297:Male; D008422:Materials Testing; D008565:Membrane Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D018345:Mice, Knockout; D036103:Nanotechnology; D016133:Polymerase Chain Reaction; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics*
D007527:Isoenzymes; D008565:Membrane Proteins; D004247:DNA; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases; C496512:Ptgs1 protein, mouse