Dissection of cauliflower mosaic virus transactivator/viroplasmin reveals distinct essential functions in basic virus replication.
Cauliflower mosaic virus (CaMV) transactivator/viroplasmin (Tav) is an essential multifunctional viral protein. Dissection of Tav by deletion mutagenesis revealed that the central region is essential for CaMV replication in single cells but that the N- and C-terminal parts are not. Strains with mutations in the central region were defective in the translational transactivator function and could be complemented by coexpressing Gag (capsid protein precursor) and Pol (polyprotein with protease, reverse transcriptase, and RNase H activity) from separate monocistronic plasmids. In contrast, total omission of Tav was only partially complemented by Gag and Pol overexpression from separate plasmids. These results indicate that CaMV basic replication requires both Tav-activated polycistronic translation and some posttranslational function(s) of Tav that is not affected by the deletions in the central region of Tav.
Kobayashi
Kappei
K
Friedrich Miescher Institute, CH-4002 Basel, Switzerland.
Hohn
Thomas
T
D001483:Base Sequence; D017796:Caulimovirus / Q000235:genetics / Q000502:physiology*; D004279:DNA, Viral / Q000096:biosynthesis*; D017353:Gene Deletion; D008969:Molecular Sequence Data; D016296:Mutagenesis; D014176:Protein Biosynthesis; D015534:Trans-Activators / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014779:Virus Replication
D004279:DNA, Viral; D015534:Trans-Activators; D014764:Viral Proteins; C065790:viroplasmin protein, Cauliflower mosaic virus