Antinociceptive effect of Phlomis olivieri Benth., Phlomis anisodonta Boiss. and Phlomis persica Boiss. total extracts.
In this study total extracts of three species of Phlomis (Lamiaceae), Phlomis olivieri, Phlomis anisodonta and Phlomis persica were tested for their antinociceptive effects using the visceral writhing test model in mice. Doses of 50, 100 and 150 mg kg(-1) of total extracts from three species of Phlomis were administered intraperitoneally 30 min before acetic acid 0.7% injection. The total extracts of P. olivieri, P. anisodonta and P. persica, respectively at doses of 150, 150 and 100 mg kg(-1), showed significant antinociceptive effects as indicated by 60.5, 54.3 and 56.7% (P<0.001) reduction of writhes, respectively in comparison to control. Results also showed that antinociceptive properties of P. olivieri, P. anisodonta and P. persica with ED(50) values of 88.21, 123.62 and 59.24 mg kg(-1), respectively, are comparable to that of indomethacin (5 mg kg(-1)). Preliminary phytochemical analysis by thin layer chromatography using specific reagents indicated the presence of iridoid glycosides and phenolic compounds such as flavonoid and phenylpropanoid glycosides in examined total extracts. The antinociceptive properties of examined total extracts could possibly be related to presence of iridoid glycosides and phenolic compounds.
Sarkhail
Parisa
P
Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Abdollahi
Mohammad
M
Shafiee
Abbas
A
D000700:Analgesics / Q000008:administration & dosage / Q000627:therapeutic use*; D000704:Analysis of Variance; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use; D002855:Chromatography, Thin Layer; D006027:Glycosides / Q000032:analysis; D007213:Indomethacin / Q000627:therapeutic use; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010146:Pain / Q000188:drug therapy*; D010147:Pain Measurement; D010636:Phenols / Q000032:analysis; D031345:Phlomis; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000627:therapeutic use*
D000700:Analgesics; D000894:Anti-Inflammatory Agents, Non-Steroidal; D006027:Glycosides; D010636:Phenols; D010936:Plant Extracts; D007213:Indomethacin