Expression of voltage-gated potassium channels Kv1.3 and Kv1.5 in human gliomas.
K(+) channels play an important role in glial cell proliferation and are functionally expressed in glial tumors. Because voltage-gated K(+) channel (Kv) subtypes Kv1.3 and Kv1.5 have been shown to contribute to growth-related properties of normal glia rather specifically, we investigated different human glioma samples for the expression of these channel subtypes using reverse transcriptase-PCR. Kv1.5 expression correlated with glioma entities and malignancy grades, i.e. expression was high in astrocytomas, moderate in oligodendrogliomas, and low in glioblastomas. No such correlation was evident for Kv1.3 expression. This study shows a clear differential expression of Kv1.5 in gliomas according to subtype and malignancy grade. This result corresponds to previous data on the expression of voltage-gated sodium channels in gliomas, which likewise showed a low or absent expression of channel subtypes in high-grade tumors.
Preussat
Katja
K
Institute of Pathology (Neuropathology), Friedrich Schiller University Jena, Bachstrasse 18, D-07740 Jena, Germany.
Beetz
Christian
C
Schrey
Michael
M
Kraft
Robert
R
Wölfl
Stefan
S
Kalff
Rolf
R
Patt
Stephan
S
D000328:Adult; D000368:Aged; D001254:Astrocytoma / Q000378:metabolism; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002648:Child; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D005909:Glioblastoma / Q000235:genetics / Q000378:metabolism; D005910:Glioma / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D051664:Kv1.3 Potassium Channel; D051666:Kv1.5 Potassium Channel; D008297:Male; D008875:Middle Aged; D009837:Oligodendroglioma / Q000235:genetics / Q000378:metabolism; D015221:Potassium Channels / Q000096:biosynthesis* / Q000235:genetics; D024642:Potassium Channels, Voltage-Gated
C496973:KCNA3 protein, human; C496981:KCNA5 protein, human; D051664:Kv1.3 Potassium Channel; D051666:Kv1.5 Potassium Channel; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated