Total protein measurement using a fiber-optic evanescent wave-based biosensor.
A novel method and instrumental system to determine the total protein concentration in a liquid sample is described. It uses a fiber optic total protein sensor (FOPS) based on the principles of fiber optic evanescent wave spectroscopy. The FOPS applies a dye-immobilized porous glass coating on a multi-mode optical fiber. The evanescent waves at the fiber optic core-cladding interface are used to monitor the protein-induced changes in the sensor element. The FOPS offers a single-step method for quantifying protein concentrations without destroying the sample. The response time and reusability of the FOPS are evaluated. This unique sensing method presents a sensitive and accurate platform for the quantification of protein.
Preejith
P V
PV
School of Mechanical and Production Engineering, c/o School of Mechanical and Production Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798.
Lim
C S
CS
Kishen
A
A
John
M S
MS
Asundi
A
A
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D003574:Cytochrome c Group / Q000032:analysis; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation* / Q000379:methods; D006454:Hemoglobins / Q000032:analysis; D055100:Optical Fibers; D010047:Ovalbumin / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D015203:Reproducibility of Results; D012394:Rosaniline Dyes / Q000032:analysis; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000032:analysis; D012996:Solutions / Q000032:analysis; D013057:Spectrum Analysis / Q000295:instrumentation* / Q000379:methods
D003574:Cytochrome c Group; D006454:Hemoglobins; D011506:Proteins; D012394:Rosaniline Dyes; D012996:Solutions; D012710:Serum Albumin, Bovine; D010047:Ovalbumin; C004692:coomassie Brilliant Blue