An influence of ABO blood group on the rate of proteolysis of von Willebrand factor by ADAMTS13.
The susceptibility of von Willebrand factor (VWF) of blood group O, A, B and AB to proteolysis by the ADAMTS13 metalloprotease was investigated. Multimeric analysis indicated that the rate of VWF proteolysis differed between blood groups and was greater for group O VWF than for non-O VWF in the rank order O >/= B > A >/= AB. Measurement of the collagen binding activity of VWF of each blood group following proteolysis for a fixed time interval corroborated the results obtained on multimer analysis: the loss of collagen binding activity was greater for VWF of group O compared with non-O VWF, in the rank order O >/= B > A >/= AB. Ristocetin was found to increase the rate of VWF proteolysis approximately two-fold; the differential between blood groups was retained in the presence of ristocetin.
Bowen
D J
DJ
Department of Haematology, University of Wales College of Medicine, Heath Park, Cardiff, UK. bowendj1@cardiff.ac.uk
D000017:ABO Blood-Group System / Q000378:metabolism*; D051722:ADAM Proteins; D000071120:ADAMTS13 Protein; D001782:Blood Donors; D003094:Collagen / Q000378:metabolism; D003720:Densitometry / Q000379:methods; D006801:Humans; D008666:Metalloendopeptidases / Q000378:metabolism*; D012310:Ristocetin / Q000494:pharmacology; D013223:Statistics as Topic; D013997:Time Factors; D014841:von Willebrand Factor / Q000032:analysis / Q000378:metabolism*
D000017:ABO Blood-Group System; D014841:von Willebrand Factor; D012310:Ristocetin; D003094:Collagen; D051722:ADAM Proteins; D008666:Metalloendopeptidases; D000071120:ADAMTS13 Protein; C099604:ADAMTS13 protein, human