Measurement of retinoids and beta-carotene 15,15'-dioxygenase activity in HR-1 hairless mouse skin with UV exposure.
We investigated the vitamin A status and beta-carotene 15,15'-dioxygenase activity in hairless mice with UV exposure to assess the regulation of vitamin A metabolism after UV irradiation. HR-1 hairless mice were irradiated with UV at 3 J/cm2 for 5 d. After UV irradiation, the mice were sacrificed and samples were obtained to analyze the retinoid concentration, expression of RXR-alpha, and beta-carotene 15,15'-dioxygenase activity. After UV exposure, the skin retinoid concentration was significantly lower as well as the expression of RXR-alpha. Higher skin beta-carotene dioxygenase activity was observed in the UV group as compared to the control group. We found no significant differences in the alpha-tocopherol concentration or acrolein levels in the skins of the two groups. In conclusion, the elevation of beta-carotene 15,15'-dioxygenase activity in hairless mice after UV exposure may be a response to reduction of the skin retinoid concentration.
Takeda
Atsuko
A
Department of Pediatrics, Osaka Medical College, 2-7 Daigakumachi, Takatsuki, Osaka 569-8686, Japan.
Morinobu
Takao
T
Takitani
Kimitaka
K
Kimura
Mieko
M
Tamai
Hiroshi
H
D000818:Animals; D015153:Blotting, Western; D008099:Liver / Q000737:chemistry; D008297:Male; D051379:Mice; D008812:Mice, Hairless; D010105:Oxygenases / Q000378:metabolism*; D018168:Receptors, Retinoic Acid / Q000032:analysis; D047488:Retinoid X Receptors; D012176:Retinoids / Q000032:analysis* / Q000097:blood; D012867:Skin / Q000737:chemistry / Q000201:enzymology* / Q000528:radiation effects*; D014157:Transcription Factors / Q000032:analysis; D014466:Ultraviolet Rays; D024502:alpha-Tocopherol / Q000032:analysis; D050559:beta-Carotene 15,15'-Monooxygenase
D018168:Receptors, Retinoic Acid; D047488:Retinoid X Receptors; D012176:Retinoids; D014157:Transcription Factors; D010105:Oxygenases; C493359:Bco1 protein, mouse; D050559:beta-Carotene 15,15'-Monooxygenase; D024502:alpha-Tocopherol