Distinct dendritic cell populations sequentially present antigen to CD4 T cells and stimulate different aspects of cell-mediated immunity.
Peptide:MHC II complexes derived from a fluorescent antigen were detected in vivo to identify the cells that present subcutaneously injected antigen to CD4 T cells. Skin-derived dendritic cells (DCs) that acquired the antigen while in the draining lymph nodes were the first cells to display peptide:MHC II complexes. Presentation by these cells induced CD69, IL-2 production, and maximal proliferation by the T cells. Later, DCs displaying peptide:MHC II complexes migrated from the injection site via a G protein-dependent mechanism. Presentation by these migrants sustained expression of the IL-2 receptor and promoted delayed type hypersensitivity. Therefore, presentation of peptide:MHC II complexes derived from a subcutaneous antigen occurs in two temporally distinct waves with different functional consequences.
Itano
Andrea A
AA
Department of Microbiology, Center for Immunology, University of Minnesota Medical School, Minneapolis, MN 55455, USA. itano001@imn.edu
McSorley
Stephen J
SJ
Reinhardt
R Lee
RL
Ehst
Benjamin D
BD
Ingulli
Elizabeth
E
Rudensky
Alexander Y
AY
Jenkins
Marc K
MK
D000818:Animals; D017951:Antigen Presentation; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002465:Cell Movement; D003713:Dendritic Cells / Q000502:physiology*; D004306:Dose-Response Relationship, Immunologic; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D006968:Hypersensitivity, Delayed / Q000209:etiology; D008198:Lymph Nodes; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D037342:Pertussis Toxin / Q000494:pharmacology
D000949:Histocompatibility Antigens Class II; D037342:Pertussis Toxin