Characterization of Adoxophyes honmai single-nucleocapsid nucleopolyhedrovirus: morphology, structure, and effects on larvae.
A nucleopolyhedrovirus (NPV) was isolated from a diseased larva of the smaller tea tortrix, Adoxophyes honmai, collected from a tea field in Tsukuba, Ibaraki, Japan. Electron microscopic observations confirmed that A. honmai NPV (AdhoNPV) was a single-nucleocapsid type virus. The genome size of AdhoNPV was estimated to be 111.6 +/- 0.9kb (mean +/- SE) by restriction endonuclease analysis. AdhoNPV was also infectious to two other Adoxophyes species, the summer fruit tortrix Adoxophyes orana and Adoxophyes dubia. The LD50 values for neonatal, second, third, fourth, and fifth (final) instar larvae of A. honmai were determined as 61, 107, 688, 1,961, and 4,085 occlusion bodies/insect, respectively. Most of the infected larvae died 5-9 days after molting to the final instar, regardless of the timing of inoculation. However, when neonates were exposed to extremely high doses of AdhoNPV (greater than 100 x LD90), larval development was prevented and most of the larvae died in the first instar.
Ishii
Takayoshi
T
Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan. ryuki@cc.tuat.ac.jp
Nakai
Madoka
M
Okuno
Shohei
S
Takatsuka
Jun
J
Kunimi
Yasuhisa
Y
D000818:Animals; D004279:DNA, Viral / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006790:Host-Parasite Interactions; D007814:Larva / Q000821:virology; D008854:Microscopy, Electron; D009036:Moths / Q000821:virology*; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology / Q000648:ultrastructure; D010572:Pest Control, Biological; D015183:Restriction Mapping; D014764:Viral Proteins / Q000032:analysis
D004279:DNA, Viral; D014764:Viral Proteins