CD69-null mice protected from arthritis induced with anti-type II collagen antibodies.
CD69, known as an early activation marker antigen on T and B cells, is also expressed on platelets and activated neutrophils, suggesting certain roles in inflammatory diseases. In order to address the role of CD69 in the pathogenesis of arthritis, we established CD69-null mice. CD69-null mice displayed a markedly attenuated arthritic inflammatory response when injected with anti-type II collagen antibodies. Cell transfer experiments with neutrophils, but not T cells or spleen cells, from wild-type mice into CD69-null mice restored the induction of arthritis. These results indicate a critical role for CD69 in neutrophil function in arthritis induction during the effector phase. Thus, CD69 would be a possible therapeutic target for arthritis in human patients.
Murata
Kaoru
K
Department of Molecular Immunology and Medical Immunology, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan.
Inami
Masamichi
M
Hasegawa
Akihiro
A
Kubo
Shuichi
S
Kimura
Motoko
M
Yamashita
Masakatsu
M
Hosokawa
Hiroyuki
H
Nagao
Tomokazu
T
Suzuki
Kazuo
K
Hashimoto
Kahoko
K
Shinkai
Hiroshi
H
Koseki
Haruhiko
H
Taniguchi
Masaru
M
Ziegler
Steven F
SF
Nakayama
Toshinori
T
D019264:Adoptive Transfer; D000818:Animals; D000843:Ankle Joint / Q000378:metabolism / Q000473:pathology; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D015703:Antigens, CD / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D001169:Arthritis, Experimental / Q000139:chemically induced* / Q000235:genetics / Q000473:pathology; D018925:Chemokines / Q000235:genetics; D024043:Collagen Type II / Q000276:immunology*; D016207:Cytokines / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D006614:Hindlimb / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D017403:In Situ Hybridization / Q000379:methods; D037181:Lectins, C-Type; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013864:Thioglycolates / Q000494:pharmacology; D000099192:CD69 Antigens
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D018925:Chemokines; D024043:Collagen Type II; D016207:Cytokines; D037181:Lectins, C-Type; D008070:Lipopolysaccharides; D013864:Thioglycolates; D000099192:CD69 Antigens