Molecular cloning and characterization of Stevia rebaudiana UDP-glucosyltransferase.
We report here the cloning and characterization of a UDP-glucose flavonoid glucosyltransferase (srUFGT) in Stevia rebaudiana. The isolated cDNA was 1419 bp in length encoding 473 deduced amino acids with a predicted molecular mass of 53.2 kDa. The products of in vitro translation from an expression vector had anthocyanidins and steviol glucosyltransferase activity. Comparison of the activity of the recombinant UDP-glucosyltransferase toward a range of acceptor substrates suggests that it may participate in the synthesis of steviol glycosides. The results support the hypothesis that the flavonoid glucosyltransferases, which have a broad substrate specificity, may be not only involved in flavonoid glucosylation but also play a role in producing the water-soluble steviol-glycosides in S. rebaudiana.
Ma
Ling Bo
LB
College of Life Science, Xiamen University, Xiamen 361005.
Zhang
Da Bing
DB
Chen
Liang
L
Chen
Mu Chuan
MC
D000595:Amino Acid Sequence; D000872:Anthocyanins / Q000378:metabolism; D003001:Cloning, Molecular; D004224:Diterpenes / Q000378:metabolism; D045786:Diterpenes, Kaurane; D017343:Genes, Plant; D005964:Glucosyltransferases / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D008969:Molecular Sequence Data; D036625:Stevia / Q000201:enzymology* / Q000235:genetics; D013379:Substrate Specificity
D000872:Anthocyanins; D004224:Diterpenes; D045786:Diterpenes, Kaurane; C012042:steviol; D005964:Glucosyltransferases; C492332:UDP-glucose flavonoid glucosyltransferase, Stevia rebaudiana