Optimization of cryopreservation of Artemisia annua L. callus.
Cryopreservation of callus tissue of Artimisia annua L. was optimized. Two lines of calli were precultured on MS medium with 5% (v/v) dimethyl sulfoxide, and protected by a cryoprotectant containing 15% (v/v) ethylene glycol, 15% (v/v) dimethyl sulfoxide, 30% (v/v) glycerol and 13.6% (w/v) sucrose. The highest survival rate of callus A201 reached 87% after it was pretreated at 25 degrees C, cryopreserved by liquid nitrogen, recovered in water bath at 25 degrees C and reloaded at 25 degrees C with 34% (w/v) sucrose solution, and that of callus A202 reached 78% after it was treated as callus A201, except pretreated at 35 degrees C, recovered at 35 degrees C and reloaded with 47.8% (w/v) sucrose solution.
Chenshu
An
A
State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China. chenshu_an@yahoo.com
Wang
Xiaodong
X
Yuan
Xiaofan
X
Zhao
Bing
B
Wang
Yuchun
Y
D031172:Artemisia annua / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D003470:Culture Media / Q000494:pharmacology; D046508:Culture Techniques / Q000379:methods*; D018515:Plant Leaves / Q000187:drug effects / Q000254:growth & development / Q000502:physiology; D011786:Quality Control; D012038:Regeneration / Q000502:physiology*
D003451:Cryoprotective Agents; D003470:Culture Media