title: Set of fluorochromophores in the wavelength range from 450 to 700 nm and suitable for labeling proteins and amino-modified DNA.
abstract: We describe the synthesis, purification, and spectral properties of new dyes and reactive labels. They absorb in the visible range between 450 and 700 nm and display analytically useful fluorescence. They were made amino-reactive by esterification with N-hydroxysuccinimide (NHS). The resulting oxysuccinimide (OSI) esters were covalently linked to the amino groups of human serum albumin (HSA) or certain DNA oligomers. Except for dyes 9 and 13, they contain one reactive group only in order to avoid cross linking of biomolecules. Labeling of amino-modified biomolecules was performed by standard protocols, and the labeled proteins and oligonucleotides were separated from the unreacted dye by gel chromatography using Sephadex G25 as the stationary phase in the case of proteins, and reversed-phase HPLC in the case of DNA oligomers. The dyes also have been used as donor-acceptor pairs in fluorescence energy transfer systems and in energy transfer cascades.
authors.affiliation: Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, D-93040, Regensburg, Germany.
authors.forename: Bianca
authors.initials: B
authors.lastname: Wetzl
authors.forename: Michaela
authors.initials: M
authors.lastname: Gruber
authors.forename: Bernhard
authors.initials: B
authors.lastname: Oswald
authors.forename: Axel
authors.initials: A
authors.lastname: Dürkop
authors.forename: Bernhard
authors.initials: B
authors.lastname: Weidgans
authors.forename: Mario
authors.initials: M
authors.lastname: Probst
authors.forename: Otto S
authors.initials: OS
authors.lastname: Wolfbeis
mesh_terms: D002855:Chromatography, Thin Layer; D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D011506:Proteins; D004247:DNA