Changes of urinary and blood porphyrin profiles by exposure to PCBs, lead or diazinon in rats.
Porphyrin profiles in excreta or blood have been useful biomarkers for monitoring exposure of hazardous xenobiotics to human or animals. We evaluated and compared the changes in urinary and blood copro-, uro-, and protoporphyrins during and after the exposure of Aroclor1254 (PCBs), lead (Pb) or diazinon to rats. PCBs (10, 50 and 100 mg/kg bw), Pb (62.5, 250 and 1,000 ppm) and diazinon (10, 30 and 90 mg/k bw) were administered to rats via gavage (PCBs and diazinon) or via drinking water (Pb) daily for 5 w. Urine and blood were collected weekly for the 5 w of exposure and for 5 w after withdrawal. Three urinary porphyrins and blood protoporphyrin increased gradually with PCB dose in a time dependent manner and remained elevated for 5 w after withdrawal. Urinary porphyrins were increased rapidly by Pb and then returned to normal immediately after Pb withdrawal while blood protoporphyrin remained high but gradualy decreased during the 5 w after withdrawal. Diazinon did not affect blood and urinary porphyrins as did PCBs and Pb, but just induced a weak increase of urinary coproporphyrin. Urinary and blood porphyrin profiles can be used as biomarkers for exposure assessment to PCBs and Pb. The normal ranges of blood porphyrins in cattle, pigs, chickens and rats were also established for use as biomarkers.
Cho
Joon-Hyoung
JH
National Veterinary Research and Quarantine Service, 480, Anyang, 430-016 Korea.
Jeong
Sang-Hee
SH
Yun
Hyo-In
HI
D000284:Administration, Oral; D000818:Animals; D002417:Cattle / Q000097:blood; D003976:Diazinon / Q000008:administration & dosage / Q000633:toxicity*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D007306:Insecticides / Q000008:administration & dosage / Q000633:toxicity*; D007854:Lead / Q000008:administration & dosage / Q000633:toxicity*; D008297:Male; D011078:Polychlorinated Biphenyls / Q000008:administration & dosage / Q000633:toxicity*; D011166:Porphyrins / Q000097:blood / Q000378:metabolism* / Q000652:urine; D051381:Rats / Q000097:blood; D013047:Specific Pathogen-Free Organisms; D013552:Swine / Q000097:blood
D007306:Insecticides; D011166:Porphyrins; D007854:Lead; D011078:Polychlorinated Biphenyls; D003976:Diazinon