[Transplantation of autologous peripheral blood stem cells for the treatment of lower limb arteriosclerosis obliterans].
OBJECTIVE
To evaluate the clinical efficacy of mobilized autologous peripheral blood stem cells (PBSC) transplantation in a 48 years old patient with lower limb arteriosclerosis obliterans (ASO).


METHODS
rhG-CSF 600 micro g/d for 5 days to mobilize stem cells. On the fifth day, PBSC were collected with a Version 4 blood-cells separator. Three hours late, the PBSC were intramuscularly injected into the ischemic areas of the two lower limbs (3 x 10(9) cells per limb). The clinical and laboratory findings were monitored every week for 3 months. Forty-four days after the implantation, left lower limb with severe ASO was given an additional implantation of the same number of cells as the first time.


RESULTS
The peripheral blood CD(34)(+) cells were increased from 0.18% to 0.75% after 5 days of rhG-CSF mobilization. Three months after the first stem cell transplantation, severe pain lameness, local cool-feeling and ulcer were improved, and ABI increased from 0.49, 0.69 to 0.50, 0.85, the amplitude of blood flow and laser Doppler blood perfusion were also significantly improved (P < 0.01). At the same time, digital subtraction angiographic scores for new collateral vessel formation were showed as + 3(rich). No related complication or adverse effect were observed during the 3-month observation.


CONCLUSIONS
Transplantation of mobilized autologous PBSC might be a simple, safe, and effective method for the treatment of ASO.
Huang
Ping-ping
PP
Institute of Hematology & Blood Diseases Hospital, National Research Center of Stem Cell Engineering & Technology, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
Li
Shang-zhu
SZ
Han
Ming-zhe
MZ
Xiao
Zhi-jian
ZJ
Yang
Ren-chi
RC
Qiu
Lu-gui
LG
Qian
Guan-qing
GQ
Fu
Ren-min
RM
Zheng
Ya-li
YL
Liu
Chun-hua
CH
Xu
Xiu-qiang
XQ
Li
Jun-fan
JF
Han
Zhong-chao
ZC
D001162:Arteriosclerosis Obliterans / Q000628:therapy*; D006801:Humans; D035002:Lower Extremity; D008297:Male; D008875:Middle Aged; D036102:Peripheral Blood Stem Cell Transplantation; D014182:Transplantation, Autologous