Interleukin-6 mediates lipopolysaccharide-induced nuclear STAT3 translocation in astrocytes of rat sensory circumventricular organs.
Pyrogenic treatment with lipopolysaccharide (LPS) or interleukin-6 (IL-6) induces nuclear STAT3 translocation in the vascular organ of the laminae terminalis (OVLT) and the subfornical organ (SFO). STAT3 immunohistochemistry was combined with the detection of marker proteins (glial, neuronal, endothelial) and the nuclear DAPI stain to determine the phenotype of responding cells. At time points with high pyrogen-induced IL-6 plasma levels, nuclear STAT3 signals were co-localized with an astrocytic cell marker. IL-6 might therefore mediate genomic activation of OVLT/SFO astrocytes during LPS-induced fever.
Harré
Eva Maria
EM
Institute of Veterinary-Physiology, Justus-Liebig-University Giessen, Frankfurter Strasse 100, D-35392 Giessen, Germany.
Roth
Joachim
J
Gerstberger
Rüdiger
R
Hübschle
Thomas
T
D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005334:Fever / Q000378:metabolism; D007031:Hypothalamus / Q000378:metabolism*; D007150:Immunohistochemistry; D015850:Interleukin-6 / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D050796:STAT3 Transcription Factor; D013356:Subfornical Organ / Q000378:metabolism*; D015534:Trans-Activators / Q000378:metabolism*
D004268:DNA-Binding Proteins; D015850:Interleukin-6; D008070:Lipopolysaccharides; D050796:STAT3 Transcription Factor; C494088:Stat3 protein, rat; D015534:Trans-Activators