[Sequence analysis of VP7 gene from rotavirus field strain from Guangzhou, China].
OBJECTIVE
To compare the difference of VP7 fragment at the nucleotide level between rotavirus Guangzhou field strain R97-196 and rotavirus Beijing field strain or prototype strains.


METHODS
The VP7 fragment amplified from Guangzhou field strain R97-196 by RT-PCR was cloned into the T-A cloning vector pUCm-T and sequenced.


RESULTS
The VP7 fragment from Guangzhou field strain R97-196 was 1,062 bp in length and contained two open reading frames which is consistent with that reported in the literature. The sequence analysis revealed that the cDNA from R97-196 shared higher nucleotide and amino acid identities with Beijing field strain T73 (98% and %, respectively) than with serotype 2-4 (G types) rotavirus (from 74% to 77% and 73% to 81%, respectively). The divergence of amino acid sequences is mainly within the nine divergence regions. The phylogenetic analysis revealed that the VP7 fragment from R97-196 was far away from rotavirus serotype G1 strain Wa.


CONCLUSIONS
The VP7 gene fragment from rotavirus Guangzhou field strain R97-196 belongs to rotavirus serotype G1. And variation of rotavirus VP7 gene fragment seems to be a geographic matter.
Chen
Dong-mei
DM
Capital Institute of Pediatrics, Beijing Municipal Laboratory of Infection and Immunity, Beijing 100020, China.
Qian
Yuan
Y
Zhang
You
Y
Chang
Ru-xu
RX
D000595:Amino Acid Sequence; D000956:Antigens, Viral; D036022:Capsid Proteins / Q000235:genetics*; D003968:Diarrhea, Infantile / Q000821:virology; D006801:Humans; D007223:Infant; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000821:virology*; D017421:Sequence Analysis; D012703:Serotyping
D000956:Antigens, Viral; D036022:Capsid Proteins; C050779:VP7 protein, Rotavirus