title: Cytotoxicity evaluation of single component dentin bonding agents.
abstract: This study evaluated the cytotoxicity of four single component dentin bonding agents: Syntac Single Component, Prime & Bond 2.1, Single Bond and One Up Bond F. The test materials were applied on dentin discs of dentin barrier models in the same way as in the clinical procedures recommended by each manufacturer. Cell viability of L 929 after exposure with the bonding agents was determined by MTT assay. The results revealed that cell survival of the first three bonding agents was 60%, while the fourth was an impressive 93%. This study showed that a total-etching bonding system is more cytotoxic than a self-etching bonding system.
authors.affiliation: Dept of Operative Dentistry, Faculty of Dentistry, Mahidol University, Bangkok, Thailand. dtlvj@mahidol.ac.th
authors.forename: La-ongthong
authors.initials: LO
authors.lastname: Vajrabhaya
authors.forename: Apaporn
authors.initials: A
authors.lastname: Pasasuk
authors.forename: Choltacha
authors.initials: C
authors.lastname: Harnirattisai
mesh_terms: D000096:Acetone / Q000737:chemistry / Q000633:toxicity; D000134:Acid Etching, Dental / Q000009:adverse effects*; D000328:Adult; D000818:Animals; D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry / Q000633:toxicity; D003782:Dental Pulp / Q000187:drug effects*; D017220:Dentin-Bonding Agents / Q000737:chemistry / Q000633:toxicity*; D015194:Diffusion Chambers, Culture; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D007739:L Cells; D008689:Methacrylates / Q000737:chemistry / Q000633:toxicity; D051379:Mice; D011109:Polymethacrylic Acids / Q000737:chemistry / Q000633:toxicity; D019279:Resin Cements / Q000737:chemistry / Q000633:toxicity*; D016369:Smear Layer
chemical_list: D017220:Dentin-Bonding Agents; D008689:Methacrylates; C446260:One-Up-Bond F; D011109:Polymethacrylic Acids; D019279:Resin Cements; C078484:Syntac dentine adhesive; C444444:prime and bond 2.1; C115612:single bond; D000096:Acetone; D017438:Bisphenol A-Glycidyl Methacrylate