title: Molecular analyses of HLA-DRB1, -DPB1, and -DQB1 in Jing ethnic minority of Southwest China.
abstract: In the present study, DNA typing for HLA-DRB1, DQB1 and DPB1 was performed using polymerase chain reaction-sequencing based typing (PCR-SBT) method in 144 random selected Jing ethnic individuals inhabiting in South China. Allele frequencies and two-locus haplotypes (DRB1-DQB1) were statistically analyzed and 20 DPB1 alleles, 27 DRB1 and 20 DQB1 were detected. The most frequent DPB1 allele was DPB1*0501 with the percentage of 36.9% followed by DPB1*1301 (15.7%), DPB1*0401 (11.0%) and DPB1*020102 (9.8%). Among the 27 detected DRB1 alleles, DRB1*120201 (13.8%) was most commonly observed followed by DRB1*150201, *030101 and *090102 alleles with the frequencies of 9.4%, 9.1% and 8.3%, respectively. Among the 20 detected DQB1 alleles the most predominant one was DQB1*030101/0309 (19.9%). DQB1*050201 (19.1%), DQB1*0201/0202 (16.1%) and DQB1*050101 (12.3%) were also frequently observed in Jing population. Statistical analysis of two-locus haplotypes showed that DRB1*120201-DQB1*030101/DRB1*120201-DQB1*0309 (HF = 9.4%, D = 6.65x10(-2)) was most predominant followed by DRB1*030101-DQB1*0201/DRB1*030101-DQB1*0202 (HF = 8.1%, D = 6.66 x 10(-2)). The comparison of HLA class II allele and haplotype frequencies in Jing with those in other populations all over the world and a dendrogram based on the DRB1, DQB1 and DPB1 genes suggested that Jing ethnic population has an origin of Southeast Asia and is belonged to the southern group of Chinese populations.
authors.affiliation: Department of Biochemistry, Key Laboratory of Genetic Engineering of MOE, College of Life Sciences, Sun Yatsen (Zhongshan) University, Guangzhou, Guangdong, People's Republic of China.
authors.forename: Jing-hai
authors.initials: JH
authors.lastname: Lin
authors.forename: Ze-huan
authors.initials: ZH
authors.lastname: Liu
authors.forename: Feng-juan
authors.initials: FJ
authors.lastname: Lv
authors.forename: Yong-gui
authors.initials: YG
authors.lastname: Fu
authors.forename: Xin-lan
authors.initials: XL
authors.lastname: Fan
authors.forename: Shi-yong
authors.initials: SY
authors.lastname: Li
authors.forename: Jian-ming
authors.initials: JM
authors.lastname: Lu
authors.forename: Xiao-yi
authors.initials: XY
authors.lastname: Liu
authors.forename: An-long
authors.initials: AL
authors.lastname: Xu
mesh_terms: D000483:Alleles; D001210:Asia, Southeastern; D044466:Asian People / Q000235:genetics*; D002681:China; D005006:Ethnicity / Q000235:genetics; D005787:Gene Frequency; D005802:Genes, MHC Class II; D005838:Genotype; D006682:HLA-DP Antigens / Q000235:genetics*; D059868:HLA-DP beta-Chains; D006683:HLA-DQ Antigens / Q000235:genetics*; D059866:HLA-DQ beta-Chains; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006801:Humans; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
chemical_list: D006682:HLA-DP Antigens; D059868:HLA-DP beta-Chains; C071170:HLA-DPB1 antigen; D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains