Glycodendritic structures based on Boltorn hyperbranched polymers and their interactions with Lens culinaris lectin.
Multivalent scaffolds bearing carbohydrates have been prepared to mediate biological processes where carbohydrates are involved. These systems consist of dendritic structures based on Boltorn H20 and H30 hyperbranched polymers to which carbohydrates are linked through a convenient spacer. Mannose has been chosen as a sugar unit to test the viability of this strategy. These glycodendritic compounds have been prepared in a few steps with good yields, showing a high solubility in physiological media and low toxicity. The binding of these dendritic polymers to the mannose-binding lectin Lens culinaris (LCA) was studied using STD-NMR experiments and quantitative precipitation assays. The results demonstrate the existence of a clear interaction between the mannose derivative systems and the Lens lectin where the dendritic scaffold does not have an important role in mannose binding but supplies the necessary multivalence for lectin cluster formation. These glycodendritic structures are able to interact with a receptor, and therefore they can be considered as promising tools for biological studies.
Arce
Eva
E
Grupo de Carbohidratos, Instituto de Investigaciones Químicas, CSIC, Isla de la Cartuja, Américo Vespucio s/n, E-41092 Sevilla, Spain.
Nieto
Pedro M
PM
Díaz
Vicente
V
Castro
Rossana García
RG
Bernad
Antonio
A
Rojo
Javier
J
D000818:Animals; D019556:COS Cells; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D008358:Mannose / Q000737:chemistry* / Q000378:metabolism; D008956:Models, Chemical; D015394:Molecular Structure; D037121:Plant Lectins / Q000737:chemistry* / Q000378:metabolism; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D013237:Stereoisomerism
C480354:BH30sucMan; D008024:Ligands; D008351:Mannans; D037121:Plant Lectins; D011108:Polymers; C033619:lentil lectin; D008358:Mannose