title: Structural characterization of Botryosphaeran: a (1-->3;1-->6)-beta-D-glucan produced by the ascomyceteous fungus, Botryosphaeria sp.
abstract: The exopolysaccharide, Botryosphaeran, produced by the ligninolytic, ascomyceteous fungus Botryosphaeria sp., was isolated from the extracellular fluid by precipitation with ethanol, and purified by gel permeation chromatography to yield a carbohydrate-rich fraction (96%) composed mainly of glucose (98%). Infra-red and 13C NMR spectroscopy showed that all the glucosidic linkages were in the beta-configuration. Data from methylation analysis and Smith degradation indicated that Botryosphaeran was a (1-->3)-beta-D-glucan with approx 22% side branching at C-6. The products obtained from partial acid hydrolysis demonstrated that the side branches consisted of single (1-->6)-beta-linked glucosyl, and (1-->6)-beta-linked gentiobiosyl residues.
authors.affiliation: Departamento de Bioquímica, Universidade Estadual de Londrina, CCE, CEP 86051-990 Londrina, PR, Brazil.
authors.forename: Aneli M
authors.initials: AM
authors.lastname: Barbosa
authors.forename: Rosângela M
authors.initials: RM
authors.lastname: Steluti
authors.forename: Robert F H
authors.initials: RF
authors.lastname: Dekker
authors.forename: Marilsa S
authors.initials: MS
authors.lastname: Cardoso
authors.forename: M L
authors.initials: ML
authors.lastname: Corradi da Silva
mesh_terms: D000107:Acetylation; D000818:Animals; D001203:Ascomycota / Q000737:chemistry / Q000378:metabolism*; D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D005936:Glucans / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification*; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D005936:Glucans; C483238:botryosphaeran