Crystallization and preliminary X-ray analysis of LC3-I.
Aut7/Apg8 is located in the intermediate structures of the autophagosome and plays an essential role in autophagosome formation. The processed form, cleaved at a C-terminus of Gly120 and called LC3-I, was expressed, purified and crystallized in two crystal forms. One form belongs to space group I4(1), with unit-cell parameters a = 84.39, c = 36.89 A. The other form belongs to space group P4(1) or P4(3), with unit-cell parameters a = 60.48, c = 35.28 A. From the latter form, a complete diffraction data set was collected to 2.1 A resolution.
Sugawara
Kenji
K
Graduate School of Pharmaceutical Sciences, Hokkaido University, N-12, W-6, Kita-ku, Sapporo 060-0812, Japan.
Suzuki
Nobuo N
NN
Fujioka
Yuko
Y
Mizushima
Noboru
N
Ohsumi
Yoshinori
Y
Inagaki
Fuyuhiko
F
D000818:Animals; D000071190:Autophagy-Related Protein 8 Family; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D004926:Escherichia coli / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000737:chemistry*; D017434:Protein Structure, Tertiary; D051381:Rats; D029701:Saccharomyces cerevisiae Proteins; D013696:Temperature; D014961:X-Ray Diffraction; D014965:X-Rays
C113757:ATG8 protein, S cerevisiae; D000071190:Autophagy-Related Protein 8 Family; D008869:Microtubule-Associated Proteins; D029701:Saccharomyces cerevisiae Proteins