Adenosine extracellular levels in human brain gliomas: an intraoperative microdialysis study.
Adenosine present in human brain glioma extracellular spaces is a marker of astrocyte purine metabolism. In this study, we evaluated adenosine levels in the extracellular fluid of 21 human gliomas of high-grade malignancy using brain microdialysis techniques coupled to high-performance liquid chromatography. The adenosine concentration (mean+/-SEM) within the control tissue was 2.99+/-0.37 microM and in the tumour tissue 1.56+/-0.46 microM. The reduction was statistically significant. It is concluded that the adenosine concentrations reached in the tumour tissue are sufficient to stimulate all adenosine receptor subtypes, suppress local anti-tumour immune responses and affect glial and endothelial cell proliferation.
Melani
Alessia
A
Department of Preclinical and Clinical Pharmacology, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy.
De Micheli
Enrico
E
Pinna
Giampietro
G
Alfieri
Alex
A
Corte
Laura Della
LD
Pedata
Felicita
F
D000241:Adenosine / Q000378:metabolism*; D000328:Adult; D000368:Aged; D001932:Brain Neoplasms / Q000378:metabolism* / Q000601:surgery; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005110:Extracellular Space / Q000378:metabolism*; D005260:Female; D005910:Glioma / Q000378:metabolism* / Q000601:surgery; D006801:Humans; D007432:Intraoperative Period / Q000379:methods; D008297:Male; D017551:Microdialysis / Q000379:methods*; D008875:Middle Aged
D000241:Adenosine