Concentrating rotavirus and recombinant-enhanced green fluorescent protein from E. coli using a pH-sensitive hydrogel.
A rotavirus and a recombinant-enhanced green fluorescent protein from E. coli were concentrated 1.7 times and 1.5 times, respectively, by ultrafiltration at 37 degrees C and pH 7 using a pH-sensitive hydrogel, poly(N-isopropylacrylamide-co-N,N'-dimethylaminopropyl methacrylamide). Recoveries were 77% and 69%, respectively, and separation efficiencies were 58% and 44%, respectively. The concentration increase of the protein was confirmed by SDS-PAGE.
Chang
Kyung Hwa
KH
Plant Metabolism Research Center, Kyung Hee University, Suwon 449-701, Korea.
Chung
In Sik
IS
Park
Chang-Ho
CH
D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics; D049452:Green Fluorescent Proteins; D006801:Humans; D020100:Hydrogels / Q000138:chemical synthesis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008164:Luminescent Proteins / Q000235:genetics / Q000302:isolation & purification*; D008567:Membranes, Artificial; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification; D012401:Rotavirus / Q000302:isolation & purification*; D014462:Ultrafiltration / Q000379:methods*
D020100:Hydrogels; D008164:Luminescent Proteins; D008567:Membranes, Artificial; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins