title: High innate production of interleukin-10 and tumor necrosis factor-alpha contributes to susceptibility for non-paraneoplastic Lambert-Eaton myasthenic syndrome.
abstract: Non-paraneoplastic Lambert-Eaton myasthenic syndrome (LEMS) is an antibody-mediated autoimmune disorder, in which genetically determined interleukin-10 (Il-10) and tumor necrosis factor-alpha (TNF-alpha) could play a role in the susceptibility for the disease. Therefore, we analyzed the production of Il-10 and TNF-alpha after whole-blood stimulation in first-degree family members of patients with LEMS without malignancy, as a measure of innate production in the patients. Thirty-six first-degree family members of 10 patients and 80 healthy controls were studied. Both Il-10 (p=0.037) and TNF-alpha (p=0.0016) production were increased in the family members, but had no relation with the severity of LEMS or HLA-B8DR3 carriership. Our findings suggest that high innate production of Il-10 and TNF-alpha is a susceptibility factor for non-paraneoplastic LEMS.
authors.affiliation: Department of Neurology, Leiden University Medical Center, P.O. Box 9600, 2300 RC, Leiden, The Netherlands. pwwirtz@lumc.nl
authors.forename: Paul W
authors.initials: PW
authors.lastname: Wirtz
authors.forename: Tom W J
authors.initials: TW
authors.lastname: Huizinga
authors.forename: Dirk-Jan
authors.initials: DJ
authors.lastname: Stoeken
authors.forename: Axel R
authors.initials: AR
authors.lastname: Wintzen
authors.forename: Jan J
authors.initials: JJ
authors.lastname: Verschuuren
mesh_terms: D000328:Adult; D004198:Disease Susceptibility / Q000276:immunology; D005260:Female; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics; D016753:Interleukin-10 / Q000096:biosynthesis*; D015624:Lambert-Eaton Myasthenic Syndrome / Q000235:genetics / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*; D015854:Up-Regulation / Q000235:genetics / Q000276:immunology*
chemical_list: D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10