A novel cross-linking reagent for bovine hemoglobin modification.
A novel cross-linking reagent, methoxypolyethelene glycol-glutamic acid, was synthesized and used to modify bovine hemoglobin. Bis-tetrameric hemoglobin with moderate affinity for O2 was obtained under the controlled reaction conditions.
He
Minglei
M
National Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box. 353, Beijing 100080, PR China.
Lu
Xiuling
X
Zhao
Dongxu
D
Su
Zhiguo
Z
D000818:Animals; D001802:Blood Substitutes / Q000138:chemical synthesis* / Q000737:chemistry; D002417:Cattle; D003432:Cross-Linking Reagents / Q000737:chemistry; D018698:Glutamic Acid / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry*; D010100:Oxygen / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*; D011485:Protein Binding
D001802:Blood Substitutes; D003432:Cross-Linking Reagents; D006454:Hemoglobins; C477046:monomethoxypolyethylene glycol-glutamic acid; D018698:Glutamic Acid; D011092:Polyethylene Glycols; D010100:Oxygen