2-Propanol degradation by Sulfolobus solfataricus.
Sulfolobus solfataricus used 2-propanol and 2-propanone (acetone) when grown in static cultures at 78 degrees C with or without glucose at 10 g l-1. The presence of 3.92 g 2-propanol l-1 in both cases inhibited growth. However, acetone accumulation following 2-propanol depletion suggested that 2-propanol was co-metabolized via the acetone metabolic pathway. Glucose at 10 g l-1 increased 2-propanol and acetone utilization from 0.93 g l-1 to 1.77 g l-1 and from 0.11 g l-1 to 1.62 g l-1, respectively. Without glucose, immobilized S. solfataricus cells increased the 2-propanol removal rate to 0.035 g l-1 h-1, compared to 0.0012 g l-1 h-1 by its suspended counterpart. The results suggest the establishment of an immobilized reactor configuration is preferential for the treatment of high temperature solvent waste streams by this acidothermophilic Crenarchaeon.
Radianingtyas
Helia
H
Biochemical Engineering and Environmental Technologies Group, Department of Chemical and Process Engineering, Heriot-Watt University, Riccarton, Edinburgh EH14 4AS, UK.
Wright
Phillip C
PC
D019840:2-Propanol / Q000378:metabolism*; D000096:Acetone / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D018914:Cells, Immobilized / Q000378:metabolism; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D012997:Solvents / Q000494:pharmacology; D016944:Sulfolobus / Q000254:growth & development / Q000378:metabolism*; D013696:Temperature
D012997:Solvents; D000096:Acetone; D005947:Glucose; D019840:2-Propanol