Voltammetric assay of azithromycin in pharmaceutical dosage forms.
The oxidative behaviour of azithromycin was studied at s glassy carbon electrode in different buffer system using cyclic, linear sweep and differential pulse voltammetry. The oxidation process was shown to be irreversible over the entire pH range studied (5-11) and was diffusion-adsorption controlled. Analytical method with adequate precision and accuracy was developed for the determination of azithromycin in phosphate buffer at pH 7 as supporting electrolyte containing 10% methanol and 0.05 M ammonium acetate. The peak current varied linearly with azithromycin concentration in the range 1-15 microg/ml. The procedure was successfully applied for assay of the drug in the pharmaceutical dosage forms. The relative standard deviation (n = 5) of 2.18% was obtained.
Nigović
Biljana
B
Faculty of Pharmacy and Biochemistry, University of Zagreb, 10000 Zagreb, Croatia. biljana@pharma.hr
Simunić
Branimir
B
D000900:Anti-Bacterial Agents / Q000032:analysis*; D017963:Azithromycin / Q000032:analysis*; D002021:Buffers; D002244:Carbon; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry / Q000295:instrumentation; D004566:Electrodes; D006863:Hydrogen-Ion Concentration; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000900:Anti-Bacterial Agents; D002021:Buffers; D002244:Carbon; D017963:Azithromycin