DNA: a programmable force sensor.
Direct quantification of biomolecular interaction by single-molecule force spectroscopy has evolved into a powerful tool for materials and life sciences. We introduce an approach in which the unbinding forces required to break intermolecular bonds are measured in a differential format by comparison with a known reference bond (here, a short DNA duplex). In addition to a marked increase in sensitivity and force resolution, which enabled us to resolve single-base pair mismatches, this concept allows for highly specific parallel assays. This option was exploited to overcome cross-reactions of antibodies in a protein biochip application.
Albrecht
Christian
C
Nanotype GmbH, Lochhamer Schlag 12, 82166 Gräfelfing, Germany.
Blank
Kerstin
K
Lalic-Mülthaler
Mio
M
Hirler
Siegfried
S
Mai
Thao
T
Gilbert
Ilka
I
Schiffmann
Susanne
S
Bayer
Tom
T
Clausen-Schaumann
Hauke
H
Gaub
Hermann E
HE
D000818:Animals; D000906:Antibodies; D020137:Base Pair Mismatch; D015374:Biosensing Techniques; D002232:Carbocyanines; D003429:Cross Reactions; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004129:Dimethylpolysiloxanes; D005453:Fluorescence; D005456:Fluorescent Dyes; D005898:Glass; D006801:Humans; D007118:Immunoassay; D015848:Interleukin-5 / Q000032:analysis / Q000276:immunology; D051379:Mice; D018625:Microscopy, Atomic Force; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D040081:Protein Array Analysis; D011485:Protein Binding; D012828:Silicones; D013696:Temperature; D013816:Thermodynamics
D000906:Antibodies; D002232:Carbocyanines; D004129:Dimethylpolysiloxanes; D005456:Fluorescent Dyes; D015848:Interleukin-5; D009838:Oligodeoxyribonucleotides; D012828:Silicones; C085321:cyanine dye 5; C013830:baysilon; D004247:DNA