[Heterogeneity of HIV strains isolated from different tissues of 3 AIDS patients].
BACKGROUND
To reveal the characteristics of genotype and phenotype of HIV strains in blood and some tissues of AIDS patients.


METHODS
The virus was isolated from peripheral blood mononuclear cell (PBMC),cerebrospinal fluid (CSF)and lymph nodes of 3 AIDS patients by coculture with PBMC stimulated by PHA for 72 hours from uninfected donor. The cytopathic effect of the HIV isolates was determined in cultured MT2 cell line. The env gene sequences form proviral DNA were analyzed by GCG software.


RESULTS
In one patient,there were differences between the strains from blood and different tissues both in genotype and phenotype. The biological phenotypes of two strains from CSF were non syncytium (NSI) type, their env sequences were similar to standard CNS tropic strain (SF162).


CONCLUSIONS
The viral heterogeneity exists in different body compartments within an infected individual. The neurotropic isolate which is similar to international standard strain exists in some AIDS patients in China.
Zhang
Chen-yang
CY
Beijing Ditan Hospital, Beijing 100011, China.
Jiang
Yan
Y
Xing
Hui
H
Feng
Yi
Y
Pan
Pin-liang
PL
Fan
Xiu-juan
XJ
Zhang
Hui
H
Liu
De-gong
DG
Shao
Yi-ming
YM
D000163:Acquired Immunodeficiency Syndrome / Q000821:virology*; D000328:Adult; D018920:Coculture Techniques; D005260:Female; D018740:Genetic Heterogeneity; D005838:Genotype; D006678:HIV / Q000302:isolation & purification*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology; D008198:Lymph Nodes / Q000821:virology; D008297:Male; D010641:Phenotype