[Inhibition of duck hepatitis B virus DNA replication by antisense phosphorothioate oligodeoxynucleotides in vitro and in vivo].
BACKGROUND
To determine the feasibility of inhibition of duck hepatitis B virus (DHBV) DNA replication by antisense phosphorothioate oligodeoxynucleotides corresponding to DHBV transcription region.


METHODS
The authors designed three antisense phosphorothioate oligodeoxynucleotides which correspond to DHBV PreS1,PreS2 and S antigen gene promotors respectively. The DNA replication level was detected with Southern blot method and cpm calculation.


RESULTS
Primary duck hepatocyte culture was treated with 1.5 micromol/L antisense oligodeoxynucleotides in vitro, all the antisense fragments caused a firm inhibition of viral DNA replication and the inhibition rates were 61.5%, 69.3% and 62.4%, respectively. In vivo, the animals were treated with 10 microgram/g PreS1 antigen gene promotor antisense oligodeoxynucleotides per day for 6 days and a very strong inhibition rate of 87.9% was obtained.


CONCLUSIONS
The results demonstrated the potential clinical application of antisense phosphorothioate oligodeoxynucleotides in clinics.
Dong
Biao
B
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical University, Beijing 100050, China.
Shao
Xing-wu
XW
Tao
Pei-zhen
PZ
D000818:Animals; D004261:DNA Replication / Q000187:drug effects*; D004279:DNA, Viral / Q000187:drug effects; D004372:Ducks; D018347:Hepadnaviridae Infections / Q000821:virology*; D006514:Hepatitis B Surface Antigens / Q000097:blood; D015611:Hepatitis B Virus, Duck / Q000235:genetics* / Q000502:physiology; D006524:Hepatitis, Viral, Animal / Q000821:virology*; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D011498:Protein Precursors / Q000097:blood; D014779:Virus Replication / Q000187:drug effects
D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D020319:Oligodeoxyribonucleotides, Antisense; D011498:Protein Precursors; C050806:presurface protein 1, hepatitis B surface antigen; C050807:presurface protein 2, hepatitis B surface antigen