Induction of cytotoxicity and ssDNA breaks by 9-bromo-5-morpholino-tetrazolo[1,5-c]quinazoline in tumor cells cultured in vitro.
9-Bromo-5-morpholino-tetrazolo[1,5-c]quinazoline (BMTQ) acted cytotoxically on murine leukemia cell line L1210 and human colon carcinoma cells Caco-2. We found the two highest concentrations of BMTQ (149.2 and 74.6 microM) induced an acute cytotoxic effect, however other tested concentrations (<74.6 microM) manifested a concentration/dependent and time/dependent cytotoxic effect. The sensitivity of murine leukemia cells L1210 and human colon carcinoma cells Caco-2 was expressed in the same order. The cytotoxicity of BMTQ was not accompanied by changes of the cell cycle profile. Following the cytotoxicity-related effects of BMTQ we observed the induction of ssDNA breaks after BMTQ treatment. All the concentrations of BMTQ increased the level of ssDNA breaks 1.3-2.9 times (after 2 h of treatment) and 1.6-2.8 times (after 4 h of treatment) in Caco-2 cells compared to the control. No apoptotic DNA fragmentation induced by BMTQ in Caco-2 cells was recorded.
Jantová
S
S
Department of Biochemistry and Microbiology, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia. jantova@chtf.stuba.sk
Cipák
L'
L
Slamenová
D
D
Horváth
V
V
Rauko
P
P
D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects; D018938:Caco-2 Cells; D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay; D004277:DNA, Single-Stranded / Q000187:drug effects*; D006801:Humans; D007939:Leukemia L1210 / Q000188:drug therapy; D051379:Mice; D011799:Quinazolines / Q000633:toxicity*; D013777:Tetrazoles / Q000633:toxicity*; D014407:Tumor Cells, Cultured
C110549:9-bromo-5-morpholino-tetrazole(1,5-c)quinazoline; D000970:Antineoplastic Agents; D004277:DNA, Single-Stranded; D011799:Quinazolines; D013777:Tetrazoles