The effect of stress upon hydrolysis adenine nucleotides in blood serum of rats.
Alterations of enzyme activities involved in adenine nucleotide hydrolysis have been reported in spinal cord and blood serum after repeated restraint stress. On the other hand, no effect was observed in the spinal cord of rats after acute stress. In the present study, we investigated the effect of acute stress on the hydrolysis of adenine nucleotides in rat blood serum. Adult male Wistar rats were submitted to 1-h restraint stress and were sacrificed at 0, 6, 24 and 48 h. Increased ATP and ADP hydrolysis were observed in the blood serum of stressed rats 24 h after stress (58% and 54%, respectively, when compared to controls). On the other hand, the AMP hydrolysis was increased after 6 h (68% when compared to controls) and at 24 h (94% when compared to controls) after stress. The results suggest that altered activity of soluble enzymes in serum may be a biochemical marker for stress situations.
Böhmer
Ana Elisa
AE
Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grando do Sul, Ramiro Barcelos, 2600 Anexo, Lab. 22, 90035-003 Porto Alegre, RS, Brazil.
Fürstenau
Cristina Ribas
CR
Torres
Iraci Lucena S
IL
Crema
Leonardo
L
Battastini
Ana Maria O
AM
Dalmaz
Carla
C
Ferreira
Maria Beatriz C
MB
Sarkis
João J F
JJ
D015720:5'-Nucleotidase / Q000097:blood; D000227:Adenine Nucleotides / Q000097:blood*; D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000097:blood; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001081:Apyrase / Q000097:blood; D006868:Hydrolysis; D008297:Male; D009708:Nucleotidases / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D012149:Restraint, Physical; D013315:Stress, Psychological / Q000378:metabolism*
D000227:Adenine Nucleotides; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D009708:Nucleotidases; C018775:adenosine monophosphatase; D015720:5'-Nucleotidase; D000251:Adenosine Triphosphatases; D001081:Apyrase