Detailed Hylobates lar karyotype defined by 25-color FISH and multicolor banding.
A comprehensive and detailed comparative chromosome map of the white-handed gibbon (Hylobates lar = HLA) has been established by hybridizing the recently developed complete human multicolor banding (MCB) probe set on metaphase chromosomes of a male HLA lymphoblastoid cell line. Thus, it was possible to precisely determine the breakpoints and distribution plus orientation of specific DNA-regions in this cytogenetically highly rearranged species compared to Homo sapiens (HSA). In general, the obtained results are in concordance with previous molecular-cytogenetic studies. In this study all 71 breakpoints present in HLA compared to HSA could be determined exactly. This study is a valuable complement to our knowledge on the phylogeny of huminoid chromosomes.
Mrasek
Kristin
K
Institute of Human Genetics and Anthropology, D-07740 Jena, Germany.
Heller
Anita
A
Rubtsov
Nikolai
N
Trifonov
Vladimir
V
Starke
Heike
H
Claussen
Uwe
U
Liehr
Thomas
T
D000818:Animals; D002871:Chromosome Banding / Q000379:methods*; D019457:Chromosome Breakage; D020223:Chromosome Painting; D003116:Color; D015342:DNA Probes / Q000235:genetics; D006801:Humans; D006922:Hylobates / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007621:Karyotyping; D008297:Male
D015342:DNA Probes