title: Electroacupuncture attenuates both glutamate release and hyperemia after transient ischemia in gerbils.
abstract: Although many studies have indicated that electroacupuncture (EA) provides a neuroprotective effect against ischemic brain damage, the protective mechanism is not fully understood. Glutamate release and hippocampal blood flow in ischemia with EA were investigated to elucidate the neuroprotective mechanism of EA. Transient 5-minute ischemia was induced in gerbils. EA (7 Hz, 6 mA, for 30 minutes) delivered to the points called Fengfu (GV16) and Shendao (GV11) was administered pre-, intra- or post-ischemia. The procedure rescued hippocampal neurons from ischemic insult and significantly attenuated both ischemia-induced glutamate release and transient increase of cerebral blood flow (CBF) during reperfusion (hyperemia). Hyperemia as well as excessive glutamate after ischemia are regarded as important factors in brain damage as they lead to reperfusion injury. These results suggest that EA protects neurons bysuppressing both glutamate release and reperfusion injury after ischemia.
authors.affiliation: Department of Neurobiology, Kagawa Medical University, Ikenobe, Miki-cho, Kagawa 761-0793, Japan.
authors.forename: Jinning
authors.initials: J
authors.lastname: Pang
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Itano
authors.forename: Kaznori
authors.initials: K
authors.lastname: Sumitani
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Negi
authors.forename: Osamu
authors.initials: O
authors.lastname: Miyamoto
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology; D002452:Cell Count; D002560:Cerebrovascular Circulation / Q000502:physiology; D015671:Electroacupuncture; D005110:Extracellular Space / Q000378:metabolism; D005849:Gerbillinae; D018698:Glutamic Acid / Q000378:metabolism*; D006624:Hippocampus / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D006940:Hyperemia / Q000503:physiopathology / Q000628:therapy*; D002546:Ischemic Attack, Transient / Q000378:metabolism / Q000503:physiopathology / Q000628:therapy*; D009474:Neurons / Q000502:physiology
chemical_list: D018698:Glutamic Acid