Temporal variations of adhesion molecules and matrix metalloproteinases in the course of MS.
Thirty patients with clinically isolated syndromes (CIS) were evaluated at the onset of neurological symptoms and when they developed clinically definite MS (CDMS). Surface expression of LFA-1alpha, VLA-4 and intercellular adhesion molecule-1 (ICAM-1) on PBMC and CSF cells was evaluated using flow cytometry. Serum and CSF concentrations of soluble vascular cell adhesion molecules-1 (VCAM-1), ICAM-1 and E-Selectin, as well as MMP-9 and MMP-2 serum concentrations were assayed using ELISA. Surface expression of LFA-1alpha and VLA-4 molecules on peripheral blood and CSF T cells and monocytes from CIS and CDMS was significantly increased compared with control subjects. Moreover, LFA-1alpha and VLA-4 expression was significantly higher in patients who developed CDMS compared with those with CIS. Similar changes were observed in the serum levels of MMP-9. Furthermore, patients with CIS and CDMS had significantly higher levels of CSF sVCAM and s-E-Selectin than control subjects. These data suggest that VLA-4, LFA-1alpha and MMP-9 play a leading role in the evolution of inflammatory demyelinating lesions in patients with CIS who develop CDMS.
Correale
Jorge
J
Department of Neurology, Raúl Carrea Institute for Neurological Research (FLENI), Montañeses 2325 (1428), Buenos Aires, Argentina. jcorreale@fleni.org.ar
Bassani Molinas
María de los Milagros
Mde L
D000328:Adult; D015815:Cell Adhesion Molecules / Q000096:biosynthesis* / Q000097:blood / Q000134:cerebrospinal fluid; D002462:Cell Membrane / Q000201:enzymology / Q000378:metabolism; D005260:Female; D006801:Humans; D039041:Integrin alpha4beta1 / Q000096:biosynthesis / Q000097:blood; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis / Q000097:blood / Q000134:cerebrospinal fluid; D008137:Longitudinal Studies; D016169:Lymphocyte Function-Associated Antigen-1 / Q000096:biosynthesis / Q000097:blood / Q000134:cerebrospinal fluid; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis / Q000097:blood; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis / Q000097:blood; D020782:Matrix Metalloproteinases / Q000096:biosynthesis* / Q000097:blood; D008875:Middle Aged; D009000:Monocytes / Q000201:enzymology / Q000378:metabolism; D009103:Multiple Sclerosis / Q000097:blood / Q000134:cerebrospinal fluid / Q000201:enzymology* / Q000378:metabolism*; D012995:Solubility; D016176:T-Lymphocyte Subsets / Q000201:enzymology / Q000378:metabolism; D013997:Time Factors; D015854:Up-Regulation
D015815:Cell Adhesion Molecules; D039041:Integrin alpha4beta1; D016169:Lymphocyte Function-Associated Antigen-1; D018799:Intercellular Adhesion Molecule-1; D020782:Matrix Metalloproteinases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9