Temperature-sensitive mutation in yeast mitochondrial ribosome recycling factor (RRF).
The yeast protein Rrf1p encoded by the FIL1 nuclear gene bears significant sequence similarity to Escherichia coli ribosome recycling factor (RRF). Here, we call FIL1 Ribosome Recycling Factor of yeast, RRF1. Its gene product, Rrf1p, was localized in mitochondria. Deletion of RRF1 leads to a respiratory incompetent phenotype and to instability of the mitochondrial genome (conversion to rho(-)/rho(0) cytoplasmic petites). Yeast with intact mitochondria and with deleted genomic RRF1 that harbors a plasmid carrying RRF1 was prepared from spores of heterozygous diploid yeast. Such yeast with a mutated allele of RRF1, rrf1-L209P, grew on a non-fermentable carbon source at 30 but not at 36 degrees C, where mitochondrial but not total protein synthesis was 90% inhibited. We propose that Rrf1p is essential for mitochondrial protein synthesis and acts as a RRF in mitochondria.
Teyssier
Emeline
E
Department of Biochemistry and Molecular Pharmacology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107-5541, USA.
Hirokawa
Go
G
Tretiakova
Anna
A
Jameson
Bradford
B
Kaji
Akira
A
Kaji
Hideko
H
D002455:Cell Division / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D004579:Electron Transport / Q000235:genetics; D017353:Gene Deletion; D005816:Genetic Complementation Test; D008928:Mitochondria / Q000235:genetics / Q000502:physiology; D024101:Mitochondrial Proteins / Q000235:genetics*; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D013696:Temperature
D004272:DNA, Mitochondrial; D024101:Mitochondrial Proteins; C114727:RRF1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins