Selective enrichment of Geobacter sulfurreducens from anaerobic granular sludge with quinones as terminal electron acceptors.
A quinone-respiring, enrichment culture derived from methanogenic granular sludge was phylogenetically characterized by using a combined cloning-denaturing gradient gel electrophoresis (DGGE) method, which revealed that the consortium developed was dominated by a single microorganism: 97% related, in a sequence of 1520 base pairs, to Geobacter sulfurreducens. The enrichment culture could grow with acetate, formate or H2 when humic acids, the humic model compound, anthraquinone-2,6-disulfonate (AQDS), or chelated Fe(III) was provided as a terminal electron acceptor. The occurrence of a humic acid- or quinone-respiring microorganism in the microbial community of a wastewater treatment system suggests that this type of microorganisms may play a potential role in anaerobic bioreactors treating humus-containing wastewaters.
Cervantes
Francisco J
FJ
Sub-Department of Environmental Technology, Wageningen University, P.O. Box 8129, 6700 EV Wageningen, The Netherlands. fcervantes@yaqui.itson.mx
Duong-Dac
Tuan
T
Ivanova
Anna E
AE
Roest
Kees
K
Akkermans
Antoon D L
AD
Lettinga
Gatze
G
Field
Jim A
JA
D000085:Acetates / Q000378:metabolism*; D000693:Anaerobiosis; D000880:Anthraquinones / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods; D020552:Comet Assay / Q000379:methods; D003470:Culture Media / Q000378:metabolism; D020564:Deltaproteobacteria / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism* / Q000502:physiology; D004579:Electron Transport; D005561:Formates / Q000378:metabolism; D006812:Humic Substances / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism; D010084:Oxidation-Reduction; D010802:Phylogeny; D011809:Quinones / Q000378:metabolism; D016415:Sequence Alignment / Q000379:methods; D017421:Sequence Analysis / Q000379:methods; D012722:Sewage / Q000382:microbiology*; D012988:Soil Microbiology; D013379:Substrate Specificity
D000085:Acetates; D000880:Anthraquinones; D003470:Culture Media; D005561:Formates; D006812:Humic Substances; D011809:Quinones; D012722:Sewage; C408124:anthraquinone-2,6-disulfonate; C030544:formic acid; D006859:Hydrogen