P/CAF and GCN5 acetylate the AML1/MDS1/EVI1 fusion oncoprotein.
Lysine acetyltransferases modulate the activity of many genes by modifying the lysine residues of both core histones and transcription-related factors. These modifications are tightly controlled in the cell because they are involved in vital processes such as cell cycle progression, differentiation, and apoptosis. Therefore, any deregulation of acetylation/deacetylation equilibrium or inappropriate modifications could lead to different diseases. Since previous studies have shown that some oncoproteins also undergo this modification, acetylation could be involved in the processes of cell transformation and oncogenesis. Here, we report that AML1/MDS1/EVI1 (AME), a repressor produced by the t(3;21) associated with human leukemia, physically interacts with the acetyltransferases P/CAF and GCN5. Our data suggest that AME has at least two binding sites for these acetyltransferases, one of which is in the Runt domain. Both P/CAF and GCN5 efficiently acetylate AME in vivo in the central region. AME acetylation has no effect on its interaction with the co-repressor CtBP1. Finally, we demonstrate that the co-expression of AME and either P/CAF or GCN5 abrogates the repression of an AML1-dependent reporter gene.
Senyuk
Vitalyi
V
Department of Pathology, Molecular Biology Research Building, M/C 737, University of Illinois at Chicago, 900 South Ashland Avenue, Chicago, IL 60607, USA.
Sinha
Kislay Kumar
KK
Chakraborty
Soumen
S
Buonamici
Silvia
S
Nucifora
Giuseppina
G
D000107:Acetylation; D000123:Acetyltransferases / Q000378:metabolism*; D000429:Alcohol Oxidoreductases; D000818:Animals; D001665:Binding Sites; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002460:Cell Line; D002467:Cell Nucleus / Q000737:chemistry; D050676:Core Binding Factor Alpha 2 Subunit; D004268:DNA-Binding Proteins / Q000378:metabolism; D005786:Gene Expression Regulation; D051548:Histone Acetyltransferases; D006801:Humans; D051379:Mice; D015514:Oncogene Proteins, Fusion / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000235:genetics; D012097:Repressor Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D015534:Trans-Activators / Q000378:metabolism*; D014157:Transcription Factors; D050880:p300-CBP Transcription Factors; D000099224:p300-CBP-Associated Factor
D000123:Acetyltransferases; D000429:Alcohol Oxidoreductases; D018797:Cell Cycle Proteins; D050676:Core Binding Factor Alpha 2 Subunit; D004268:DNA-Binding Proteins; D051548:Histone Acetyltransferases; D015514:Oncogene Proteins, Fusion; D010750:Phosphoproteins; D016186:Receptor, Macrophage Colony-Stimulating Factor; D012097:Repressor Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D050880:p300-CBP Transcription Factors; D000099224:p300-CBP-Associated Factor; C460199:AML1-MDS1-EVI1 fusion protein, human; C096794:C-terminal binding protein; C501178:Gcn5l2 protein, mouse; C501179:KAT2A protein, human