Different sensitivity of Ca(2+)-ATPase and cholinesterase to pure and commercial pesticides in nervous ganglia of Phyllocaulis soleiformis (Mollusca).
We measured the effects in vitro of pure and commercial pesticides on Ca(2+)-activated ATPase and cholinesterase (ChE) activities in the nervous system of the slug Phyllocaulis soleiformis. The pesticides used in this study included carbamate and organophosphates, which acts as reversible and irreversible anticholinesterases, respectively. Both enzymes were insensitive to pure carbofuran (1 mM), glyphosate (1 mM) and malathion (120 microM). However, the carbamate carbofuran, in the commercial formulation Furandan 350S, inhibited ATPase and ChE activities. The organophosphate glyphosate used in the commercial preparation of Gliz 480CS inhibited ATPase activity and increased cholinesterase activity. These effects are likely due to the action of adjuvant substances of the chemical formulation. The commercial formulation (Malatol 500CE) did not alter enzymes activities. Our results suggest that cholinesterase present in the slug nervous tissue has a different behavior to those identified in vertebrate nervous tissue, since it was insensitive to pure compounds, known as anticholinesterases in vertebrates. Considering the insensitivity of the Ca(2+)-activated ATPase, we suggested that the purinergic neurotransmission and other roles of ATP might not be affected by the pure pesticides tested.
Souza da Silva
Rosane
R
Departamento de Ciências Fisiológicas, Laboratório de Pesquisa Bioquímica, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Avenida Ipiranga, 6681, Caixa Postal 1429, Porto Alegre, RS 90619-900, Brazil.
Cognato
Giana de Paula
Gde P
Vuaden
Fernanda Cenci
FC
Rezende
Maria Fernanda S
MF
Thiesen
Flávia Valladão
FV
Fauth
Maria da Graça
Mda G
Bogo
Maurício Reis
MR
Bonan
Carla Denise
CD
Dias
Renato Dutra
RD
D000818:Animals; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D002235:Carbofuran / Q000494:pharmacology; D002802:Cholinesterases / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D017952:Ganglia, Invertebrate / Q000187:drug effects* / Q000201:enzymology*; D005998:Glycine / Q000031:analogs & derivatives* / Q000494:pharmacology; D008974:Mollusca / Q000187:drug effects* / Q000201:enzymology; D010575:Pesticides / Q000494:pharmacology*; D000097797:Glyphosate
D000252:Calcium-Transporting ATPases; D002235:Carbofuran; D002802:Cholinesterases; D004791:Enzyme Inhibitors; D005998:Glycine; D010575:Pesticides; D000097797:Glyphosate