Identification of transcription coactivator OCA-B-dependent genes involved in antigen-dependent B cell differentiation by cDNA array analyses.
The tissue-specific transcriptional coactivator OCA-B is required for antigen-dependent B cell differentiation events, including germinal center formation. However, the identity of OCA-B target genes involved in this process is unknown. This study has used large-scale cDNA arrays to monitor changes in gene expression patterns that accompany mature B cell differentiation. B cell receptor ligation alone induces many genes involved in B cell expansion, whereas B cell receptor and helper T cell costimulation induce genes associated with B cell effector function. OCA-B expression is induced by both B cell receptor ligation alone and helper T cell costimulation, suggesting that OCA-B is involved in B cell expansion as well as B cell function. Accordingly, several genes involved in cell proliferation and signaling, such as Lck, Kcnn4, Cdc37, cyclin D3, B4galt1, and Ms4a11, have been identified as OCA-B-dependent genes. Further studies on the roles played by these genes in B cells will contribute to an understanding of B cell differentiation.
Kim
Unkyu
U
Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.
Siegel
Rachael
R
Ren
Xiaodi
X
Gunther
Cary S
CS
Gaasterland
Terry
T
Roeder
Robert G
RG
D000818:Animals; D000941:Antigens; D001402:B-Lymphocytes / Q000166:cytology* / Q000378:metabolism*; D001483:Base Sequence; D002454:Cell Differentiation / Q000235:genetics; D002455:Cell Division / Q000235:genetics; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling; D051660:Intermediate-Conductance Calcium-Activated Potassium Channels; D008213:Lymphocyte Activation; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck) / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout; D020411:Oligonucleotide Array Sequence Analysis; D015221:Potassium Channels / Q000235:genetics; D024681:Potassium Channels, Calcium-Activated; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism; D015398:Signal Transduction; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D015534:Trans-Activators / Q000235:genetics*
D000941:Antigens; D018076:DNA, Complementary; D051660:Intermediate-Conductance Calcium-Activated Potassium Channels; C496925:Kcnn4 protein, mouse; D015221:Potassium Channels; D024681:Potassium Channels, Calcium-Activated; C490424:Pou2af1 protein, mouse; D011947:Receptors, Antigen, B-Cell; D015534:Trans-Activators; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck)