Cytoprotection by pro-vitamin C against ischemic injuries in perfused rat heart together with differential activation of MAP kinase family.
The cardiac muscle cells are known to be killed by ischemia-reperfusion (I/R) treatment that produce reactive oxygen species (ROS). We analyzed the function of the autooxidation-resistant pro-vitamin C, 2-O-alpha-D-glucosylated derivative (Asc2G) of ascorbic acid (Asc), in protecting against I/R injury of the heart in rat. The serum release of the intracellular enzyme CPK due to I/R injury decreased upon injection with Asc2G. Out of the mitogen-activated protein (MAP) kinase family members, MAP kinase and JNK underwent the down-regulation in contrast to up-regulation of p38 compared with the I/R-treated control in the absence of Asc2G. These data suggest important roles for differential activation of the MAP kinase family in cytoprotection against I/R injury by Asc2G.
Eguchi
Masahiro
M
Division of Cell Biochemistry, School of BioSciences, Hiroshima Prefectural University, Nanatsuka, Shobara, Hiroshima 727-0023, Japan.
Fujiwara
Mayumi
M
Mizukami
Yoichi
Y
Miwa
Nobuhiko
N
D000818:Animals; D003402:Creatine Kinase / Q000378:metabolism; D019610:Cytoprotection / Q000187:drug effects*; D015536:Down-Regulation / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D007262:Infusions, Intravenous; D008297:Male; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D017202:Myocardial Ischemia / Q000378:metabolism / Q000517:prevention & control*; D009206:Myocardium / Q000166:cytology / Q000378:metabolism; D010766:Phosphorylation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism / Q000517:prevention & control*; D013400:Sugar Acids / Q000494:pharmacology*; D015854:Up-Regulation / Q000187:drug effects
D013400:Sugar Acids; D020928:Mitogen-Activated Protein Kinases; D003402:Creatine Kinase; C011491:provitamin C