Lipid and protein oxidation contribute to a prothrombotic state in patients with type 2 diabetes mellitus.
Diabetes mellitus (DM) is associated with enhanced lipid oxidation and persistent platelet activation. We investigated whether oxidant stress (OS) also affects circulating proteins and is associated with an abnormal coagulative pattern. In 72 type 2 DM (T2DM) patients, urinary 8-iso-prostaglandin (PG) F2alpha and 11-dehydro-thromboxane B2 (TXM) were measured as markers of lipid peroxidation and platelet activation, respectively. The carbonyl content of plasma proteins (PCARB) was measured as global index of protein oxidation. 8-Iso-PGF2alpha and PCARB levels were higher in DM patients than in controls (P < 0.05). Likewise, both TXM and prothrombin F1+2 levels were higher in diabetics (P < 0.05). By contrast, anticoagulant markers, such as activated protein C, protein C activation peptide, and soluble thrombomodulin (TM) were depressed in T2DM (P < 0.05). In conclusion, OS in T2DM involves circulating proteins and is associated with an unbalanced promotion of procoagulant reactions. These effects in concert with platelet activation may contribute to atherothrombotic complications in T2DM.
De Cristofaro
R
R
Center of Excellence on Aging, G. D'Annunziou University School of Medicine and Pharmacy, Chieti, Italy. rdecristofaro@rm.unicatt.it
Rocca
B
B
Vitacolonna
E
E
Falco
A
A
Marchesani
P
P
Ciabattoni
G
G
Landolfi
R
R
Patrono
C
C
Davì
G
G
D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood / Q000652:urine; D001798:Blood Proteins / Q000378:metabolism*; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000150:complications* / Q000378:metabolism*; D015237:Dinoprost / Q000031:analogs & derivatives*; D028441:F2-Isoprostanes / Q000652:urine; D005260:Female; D006487:Hemostasis; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D015539:Platelet Activation; D013927:Thrombosis / Q000097:blood / Q000209:etiology* / Q000378:metabolism; D013929:Thromboxane B2 / Q000031:analogs & derivatives* / Q000652:urine
D015415:Biomarkers; D001798:Blood Proteins; D028441:F2-Isoprostanes; C075750:8-epi-prostaglandin F2alpha; D013929:Thromboxane B2; C049235:11-dehydro-thromboxane B2; D015237:Dinoprost