title: The effect of stress upon hydrolysis adenine nucleotides in blood serum of rats.
abstract: Alterations of enzyme activities involved in adenine nucleotide hydrolysis have been reported in spinal cord and blood serum after repeated restraint stress. On the other hand, no effect was observed in the spinal cord of rats after acute stress. In the present study, we investigated the effect of acute stress on the hydrolysis of adenine nucleotides in rat blood serum. Adult male Wistar rats were submitted to 1-h restraint stress and were sacrificed at 0, 6, 24 and 48 h. Increased ATP and ADP hydrolysis were observed in the blood serum of stressed rats 24 h after stress (58% and 54%, respectively, when compared to controls). On the other hand, the AMP hydrolysis was increased after 6 h (68% when compared to controls) and at 24 h (94% when compared to controls) after stress. The results suggest that altered activity of soluble enzymes in serum may be a biochemical marker for stress situations.
authors.affiliation: Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grando do Sul, Ramiro Barcelos, 2600 Anexo, Lab. 22, 90035-003 Porto Alegre, RS, Brazil.
authors.forename: Ana Elisa
authors.initials: AE
authors.lastname: Böhmer
authors.forename: Cristina Ribas
authors.initials: CR
authors.lastname: Fürstenau
authors.forename: Iraci Lucena S
authors.initials: IL
authors.lastname: Torres
authors.forename: Leonardo
authors.initials: L
authors.lastname: Crema
authors.forename: Ana Maria O
authors.initials: AM
authors.lastname: Battastini
authors.forename: Carla
authors.initials: C
authors.lastname: Dalmaz
authors.forename: Maria Beatriz C
authors.initials: MB
authors.lastname: Ferreira
authors.forename: João J F
authors.initials: JJ
authors.lastname: Sarkis
mesh_terms: D015720:5'-Nucleotidase / Q000097:blood; D000227:Adenine Nucleotides / Q000097:blood*; D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000097:blood; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001081:Apyrase / Q000097:blood; D006868:Hydrolysis; D008297:Male; D009708:Nucleotidases / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D012149:Restraint, Physical; D013315:Stress, Psychological / Q000378:metabolism*
chemical_list: D000227:Adenine Nucleotides; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D009708:Nucleotidases; C018775:adenosine monophosphatase; D015720:5'-Nucleotidase; D000251:Adenosine Triphosphatases; D001081:Apyrase