Modulation of the antioxidant activity of HO* scavengers by albumin binding: a 19F-NMR study.
The interaction between different HO(z.rad;) radical scavengers in a three-component antioxidant system has been investigated by means of 19F-NMR spectroscopy. This system is composed of bovine serum albumin (BSA), trolox, and N-(4-hydroxyphenyl)-trifluoroacetamide (CF(3)PAF). The antioxidant capacity of BSA and trolox has been assessed by measuring the amount of trifluoroacetamide (TFAM) arising from the radical mediated decomposition of CF(3)PAF. When assayed separately, both trolox and BSA behaved as antioxidants, as they were effective to protect CF(3)PAF from HO* radical-mediated decomposition. By contrast, trolox enhanced the production of TFAM in the presence of BSA, thus behaving as a pro-oxidant. Urate, carnosine, glucose, and propylgallate showed antioxidant properties both with or without BSA. CF(3)PAF and trolox were found to bind to BSA with association constants in the order of 5 x 10(3)M(-1) and to compete for the same binding sites. These results have been discussed in terms of BSA-catalysed cross-reactions between trolox-derived secondary radicals and CF(3)PAF.
Aime
Silvio
S
Department of Chemistry I.F.M., University of Torino, Via Pietro Giuria 7, Turin I-10125, Italy.
Digilio
Giuseppe
G
Bruno
Erik
E
Mainero
Valentina
V
Baroni
Simona
S
Fasano
Mauro
M
D000081:Acetamides / Q000737:chemistry / Q000378:metabolism; D000627:Aminophenols / Q000737:chemistry / Q000378:metabolism; D000975:Antioxidants / Q000378:metabolism*; D001667:Binding, Competitive; D002839:Chromans / Q000378:metabolism*; D005462:Fluorine Radioisotopes; D005463:Fluoroacetates; D016166:Free Radical Scavengers / Q000378:metabolism*; D017665:Hydroxyl Radical / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D014269:Trifluoroacetic Acid / Q000378:metabolism
D000081:Acetamides; D000627:Aminophenols; D000975:Antioxidants; D002839:Chromans; D005462:Fluorine Radioisotopes; D005463:Fluoroacetates; D016166:Free Radical Scavengers; C476979:N-(4-hydroxyphenyl)trifluoroacetamide; D012710:Serum Albumin, Bovine; D017665:Hydroxyl Radical; C030688:trifluoroacetamide; D014269:Trifluoroacetic Acid; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid