Combined tyramide signal amplification and quantum dots for sensitive and photostable immunofluorescence detection.
Conventional immunofluorescence detection of biologically relevant proteins and antigens in tissue sections is often limited by relatively weak signals that fade rapidly on illumination. We have developed an immunohistochemical protocol that combines the sensitivity of tyramide signal amplification with the photostability of quantum dots to overcome these limitations. This simple method provides a sensitive and stable fluorescence immunohistochemical alternative to standard chromogen detection.
Ness
Jayne M
JM
Division of Pediatric Neurology, Department of Pediatrics, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA.
Akhtar
Rizwan S
RS
Latham
Cecelia B
CB
Roth
Kevin A
KA
D000818:Animals; D001921:Brain / Q000378:metabolism; D004355:Drug Stability; D005456:Fluorescent Dyes / Q000737:chemistry; D007202:Indicators and Reagents; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity; D019809:Streptavidin / Q000737:chemistry; D014439:Tyramine
D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D019809:Streptavidin; D014439:Tyramine