Effects of transgene Oncostatin M on the development of retinal neuron in transgenic mice.
OBJECTIVE
Oncostatin M (OSM) is a cytokine released by macrophages and lymphocytes that can function as a growth regulator. A current study shows that leukemia inhibitory factor (LIF), a homologue of OSM, can prevent photoreceptor cell death when expressed in the lens of transgenic mice. We determined the efforts of lens-specific overexpression of OSM on the development of eye.


METHODS
A truncated mouse OSM cDNA (-660 bp) was linked to the alpha A-crytallin promoter, and injected into single-cell embryos with microinjection. Then, transgenic mice were established. The mRNA expression of transgene OSM was detected by in situ hybridization. Immunohistochemistry was used to detect the expression of syntaxin, glial fibrillary acidic protein (GFAP), synaptophysin in the retinas of transgenic mice.


RESULTS
At embryonic day (E 17.5), the expression of the syntaxin at the inner and mid portion of the retinas of transgenic mice was much higher than that of the retinas of non-transgenic mice. The expression of GFAP was detected in the retinas of transgenic mice, while no expression in non-transgenic normal FVB (FVB/N) mice was detected in this stage. At postnatal day one (P1), the expression of synaptophysin was detected in the retinas of transgenic mice, but there was no such expression in FVB/N mice.


CONCLUSIONS
Lens-specific overexpression of OSM induces premature differentiation of amacrine cells, gial cells, and photoreceptors in vivo.
Xia
Xiaobo
X
Department of Ophthalmology, Xiangya Hospital, Central South University, Changsha, 410008.
Chen
Qin
Q
D000818:Animals; D005260:Female; D005786:Gene Expression Regulation; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis* / Q000235:genetics; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000235:genetics; D007908:Lens, Crystalline / Q000378:metabolism; D053605:Leukemia Inhibitory Factor; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D009474:Neurons / Q000378:metabolism; D053683:Oncostatin M; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D010786:Photoreceptor Cells / Q000378:metabolism; D050765:Qa-SNARE Proteins; D012160:Retina / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D019076:Transgenes
D005904:Glial Fibrillary Acidic Protein; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008565:Membrane Proteins; C508352:Osm protein, mouse; D010455:Peptides; D050765:Qa-SNARE Proteins; D053683:Oncostatin M