Proteome changes induced by expression of tumor suppressor PTEN.
The tumor suppressor, PTEN, located at 10q23, is one of the most frequently mutated tumor suppressors in a number of sporadic cancers and in two autosomal dominant harmatomas. It is considered one of the most important tumor suppressors in the post p53 era. To identify the molecules involved in the signal network regulated by PTEN using proteomic tools, a PTEN-inducible expression system was established in NIH 3T3 mouse embryonic fibroblast cells. We compared proteome images of PTEN-induced and non-induced cells by 2-dimensional electrophoresis. Twenty-nine differentially expressed protein spots were identified by MALDI-TOF MS and NSI MS/MS. We conclude that expression of PTEN by itself leads to protein profile changes, and those proteins affected are likely to be directly and/or indirectly involved in the function and physiology of the tumor suppressor.
Kim
So-Yeon
SY
Protein Network Research Cente, Yonsei University, Seoul 120-749, Korea.
Kim
Yu Sam
YS
Bahk
Young Yil
YY
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D015870:Gene Expression; D016147:Genes, Tumor Suppressor; D051379:Mice; D041681:NIH 3T3 Cells; D020543:Proteome / Q000378:metabolism*; D013997:Time Factors; D015854:Up-Regulation
D020543:Proteome