Systemic and local cytokine response of young piglets to oral infection with Salmonella enterica serotype Typhimurium.
One-week-old breast-fed miniature piglets were orally infected either with virulent LT2 strain or with a non-virulent SF1591 rough mutant of Salmonella Typhimurium for 1 d. Both microorganisms were cultivated from mesenteric lymph nodes but not from the blood of infected piglets. Interleukins (IL) 1 beta, 8, 18, tumor necrosis factor alpha (TNF-alpha) and interferon gamma (IFN-gamma) were quantified by ELISA in plasma and washes of a terminal part of the small bowel. In plasma, cytokines were mostly missing in non-infected piglets and either missing or low in infected piglets. In the gut of non-infected piglets, IL-1 beta, IL-8 and IL-18 were detected whereas TNF-alpha and IFN-gamma were mostly missing. IFN-gamma levels highly increased (p < 0.05) after infection with nonvirulent salmonellae. The variability of cytokine levels in the gut of suckling piglets is discussed.
Trebichavský
I
I
Department of Immunology and Gnotobiology, Institute of Microbiology, Academy of Sciences of the Czech Republic, 549 22 Nový Hrádek, Czechia. treb@biomed.cas.cz
Splíchal
I
I
Splíchalová
A
A
Muneta
Y
Y
Mori
Y
Y
D000818:Animals; D000831:Animals, Newborn; D016207:Cytokines / Q000097:blood / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D005767:Gastrointestinal Diseases / Q000276:immunology / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D007082:Ileum / Q000276:immunology / Q000382:microbiology / Q000473:pathology / Q000648:ultrastructure; D008198:Lymph Nodes / Q000276:immunology / Q000382:microbiology* / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron / Q000662:veterinary; D012481:Salmonella Infections, Animal / Q000276:immunology* / Q000382:microbiology; D012486:Salmonella typhimurium / Q000254:growth & development* / Q000648:ultrastructure; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000382:microbiology* / Q000473:pathology; D013556:Swine, Miniature
D016207:Cytokines