Human herpesvirus 6 infection of human epidermal cell line: pathogenesis of skin manifestations.
In order to elucidate the pathogenesis of variant B human herpesvirus 6 (HHV-6) infection in skin tissues, an A431 cell line was inoculated with variant B HHV-6. HHV-6 causes abortive infection in the A431 cells, because neither late antigen (OHV-3 antigen) nor progeny virus is produced. Maximum levels of HHV-6 antigen (IEA/ex3 antigen)-positive cells (36.4%) were observed 48 hr after viral infection. Cocultivation of HHV-6-infected cord blood mononuclear cells with A431 cells was necessary for the establishment of a sufficient level of viral infection. Cell-to-cell contact between the infected cord blood mononuclear cells and A431 cells was crucial for increasing infection efficiency. To determine the biological effect of HHV-6 infection, flow cytometric analysis was carried out in HHV-6- and mock-infected A431 cells. Although no alteration was observed in VCAM-1 and ELAM-1 expression, that of HLA-ABC, HLA-DR, and ICAM-1 was upregulated after infection with HHV-6.
Yoshikawa
Tetsushi
T
Laboratory of Virology, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Nagoya, Aichi, Japan. tetsushi@fujita-hu.ac.jp
Goshima
Fumi
F
Akimoto
Shiho
S
Ozaki
Takao
T
Iwasaki
Takuya
T
Kurata
Takeshi
T
Asano
Yoshizo
Y
Nishiyama
Yukihiro
Y
D002460:Cell Line; D004817:Epidermis / Q000378:metabolism / Q000473:pathology* / Q000821:virology*; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D006680:HLA Antigens / Q000378:metabolism; D015654:Herpesvirus 6, Human / Q000502:physiology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism
D006680:HLA Antigens; D018799:Intercellular Adhesion Molecule-1