Effect of PCB 126 and PCB 153 on incidence of apoptosis in cultured theca and granulosa cells collected from small, medium and large preovulatory follicles.
The aim of the presented study was to evaluate the effects of PCB 126 and PCB 153 on granulosa and theca cell apoptosis. Granulosa and theca cells were collected from small, medium, and large preovulatory porcine follicles and cultured as monolayers. Cells were initially cultured for 24 h to allow attachment to the plates. Media were changed and 100 pg/ml PCB 126 or 100 ng/ml PCB 153 were added. After 48 h, granulosa and theca cells were fixed for assessment of the number of apoptotic cells utilizing a Hoechst staining technique or frozen for measurement of caspase-3 activity. Media were collected for testosterone concentration analysis from theca cell cultures or estradiol from granulosa cell cultures. Neither PCB 153 nor PCB 126 had an effect on testosterone secretion by theca cells collected from small and medium size follicles, while both PCBs decreased testosterone secretion by large follicles. The decrease in testosterone secretion by large follicles under the influence of both PCBs was paralleled by a suppression of caspase-3 activity and a decreased incidence of apoptotic bodies. Neither of the PCBs had an effect on estradiol secretion by granulosa cells collected from small and medium size follicles, while both PCBs increased estradiol in granulosa cells collected from large follicles. PCB-associated increased estradiol secretion by granulosa cells collected from large follicles was accompanied by suppression of caspase-3 activity and a decreased incidence of apoptotic bodies. In conclusion, we have presented evidence that in preovulatory follicles PCBs inhibit both theca and granulosa cells apoptosis. Therefore, an exposure to PCBs may cause alterations in the pattern of terminal differentiation of follicles and attenuate spontaneous elimination of atretic follicles.
Gregoraszczuk
E L
EL
Department of Animal Physiology, Institute of Zoology, Jagiellonian University, ul. Ingardena 6, 30-060 Krakow, Poland. greg@zuk.i
Sowa
M
M
Kajta
M
M
Ptak
A
A
Wójtowicz
A
A
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000032:analysis / Q000187:drug effects / Q000378:metabolism; D048429:Cell Size / Q000187:drug effects; D002478:Cells, Cultured; D004789:Enzyme Activation; D004958:Estradiol / Q000378:metabolism; D004965:Estrogen Antagonists / Q000633:toxicity*; D005260:Female; D005470:Fluorometry; D006107:Granulosa Cells / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D006080:Ovarian Follicle / Q000166:cytology* / Q000254:growth & development*; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D013552:Swine; D013739:Testosterone / Q000378:metabolism; D013799:Theca Cells / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*
D004965:Estrogen Antagonists; D013739:Testosterone; D004958:Estradiol; D011078:Polychlorinated Biphenyls; D020169:Caspases; C023035:3,4,5,3',4'-pentachlorobiphenyl; C014024:2,4,5,2',4',5'-hexachlorobiphenyl