title: The Escherichia coli AIDA autotransporter adhesin recognizes an integral membrane glycoprotein as receptor.
abstract: The AIDA-I autotransporter adhesin, as a prototype of the AIDA adhesin family, represents a tripartite antigen consisting of the functional adhesin AIDA-I (alpha-domain), which mediates the specific attachment of bacteria to target cells, and a two-domain translocator (AIDA(c)) organized in the beta(1)- and beta(2)-domains. Cellular receptor moieties for the adhesin AIDA-I have not been identified. Here, it is demonstrated that the purified adhesin binds specifically to a high-affinity class of receptors on HeLa cells. Additionally, the adhesin was found to bind to a variety of mammalian cell types, indicating a broad tissue distribution of the receptor moiety. By using complementary techniques, including co-immunoprecipitation and one- and two-dimensional gel electrophoresis, the AIDA-I binding protein on HeLa cells was identified as a surface glycoprotein of about 119 kDa (gp119). The gp119 AIDA-I cellular receptor protein was characterized biochemically and found to be an integral N-glycosylated membrane protein with a pI of 5.2.
authors.affiliation: Institut für Infektiologie, Zentrum für Molekularbiologie der Entzündung (ZMBE), Westfälische Wilhelms-Universität Münster, Von-Esmarch-Str. 56, 48149 Münster, Germany.
authors.forename: Sven
authors.initials: S
authors.lastname: Laarmann
authors.affiliation: Institut für Infektiologie, Zentrum für Molekularbiologie der Entzündung (ZMBE), Westfälische Wilhelms-Universität Münster, Von-Esmarch-Str. 56, 48149 Münster, Germany.
authors.forename: M Alexander
authors.initials: MA
authors.lastname: Schmidt
mesh_terms: D018830:Adhesins, Escherichia coli / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D006031:Glycosylation; D006367:HeLa Cells; D006801:Humans; D007526:Isoelectric Point; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D008970:Molecular Weight; D011233:Precipitin Tests; D017433:Protein Structure, Secondary; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000737:chemistry / Q000378:metabolism*
chemical_list: C075559:AIDA-I protein, E coli; D018830:Adhesins, Escherichia coli; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; C047105:bacterial adhesin receptor