Biosurfactant- and biodegradation-enhanced partitioning of polycyclic aromatic hydrocarbons from nonaqueous-phase liquids.
A study was conducted on the effect of two different biological factors, microbial surfactants and biodegradation, on the kinetics of partitioning of polycyclic aromatic hydrocarbons (PAHs) from nonaqueous-phase liquids (NAPLs). The effect of rhamnolipid biosurfactants on partitioning into the aqueous phase of naphthalene, fluorene, phenanthrene, and pyrene, initially dissolved in di-2-ethylhexyl phthalate (DEHP) or 2,2,4,4,6,8,8-heptamethylnonane (HMN), was determined in multiple-solute experiments. Biosurfactants at a concentration above the CMC enhanced the partitioning rate of fluorene, phenanthrene, and pyrene but were ineffective with naphthalene. Enhancement of partitioning was also observed in the presence of suspended humic acid-clay complexes, which simulated the solids often present in the subsurface. Biosurfactants sorbed to the complexes modified PAH partitioning between the NAPL and these solids, increasing the fraction of solid-phase PAH. Biodegradation-driven partitioning was estimated in mineralization experiments with phenanthrene initially present in HMN and three representative soil bacterial strains, differing in their potential adherence to the NAPL. In the three cases, the rates of mineralization were very similar and significantly higher than the abiotic rate of partitioning. Our study suggests that in NAPL-polluted sites, partitioning of PAH may be efficiently enhanced by in situ treatments involving the use of biosurfactants and biodegradation.
Garcia-Junco
Marta
M
Instituto de Recursos Naturales y Agrobiología, C.S.I.C., Apartado 1052, E-41080-Seville, Spain.
Gomez-Lahoz
Cesar
C
Niqui-Arroyo
Jose-Luis
JL
Ortega-Calvo
José-Julio
JJ
D001673:Biodegradation, Environmental; D007700:Kinetics; D008962:Models, Theoretical; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D013501:Surface-Active Agents / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000378:metabolism*
D011084:Polycyclic Aromatic Hydrocarbons; D012989:Soil Pollutants; D013501:Surface-Active Agents; D014874:Water Pollutants, Chemical