title: Monitoring of seasonal variability in bronchial hyper-responsiveness and sputum cell counts in non-asthmatic subjects with rhinitis and effect of specific immunotherapy.
abstract: Bronchial hyper-responsiveness (BHR) is documented in a proportion of non-asthmatic individuals with allergic rhinitis (NAAR) and reflects inflammatory events in the lower airways. Natural exposure to allergens is known to modulate BHR and the level of airway inflammation in asthma, but less consistently in NAAR. Specific immunotherapy (SIT) attenuates symptoms possibly by reducing BHR and airway inflammation. The influence of natural exposure to Parietaria pollen on BHR and sputum cell counts of NAAR was investigated and the effect of Parietaria SIT examined. Thirty NAAR, monosensitized to Parietaria judaica, participated in a randomized, double-blind, placebo-controlled, parallel group study of the effects of a Parietaria pollen vaccine on symptoms/medication score, BHR to inhaled methacholine and adenosine 5'-monophosphate (AMP), and cell counts in the sputum collected out of and during the pollen seasons for 36 months. Seasonal variation in BHR to inhaled methacholine and AMP and changes in sputum cell counts were documented. Changes were consistent for AMP, but not methacholine, and invariably associated with modifications in sputum eosinophils and epithelial cells. The clinical efficacy of Parietaria SIT was associated with a decline in the seasonal deterioration of BHR to AMP, whereas no significant effect was observed on BHR to methacholine or sputum cell differentials. Between-groups comparison of the seasonal changes in PC15 methacholine values and sputum cell differentials calculated as the AUC were not statistically significant, whereas a significant difference in PC15 AMP was demonstrated throughout the study (P=0.029), the median (inter-quartile range) AUC values being 2478.5 (1153.3-3600.0) and 1545.5 (755.3-1797.9) for the SIT- and placebo-treated group, respectively. Bronchial airways of NAAR exhibit features of active inflammation that deteriorate during natural allergen exposure, particularly with regard to BHR to AMP. The clinical efficacy of Parietaria SIT was exclusively associated with attenuation in seasonal worsening of PC15 AMP, suggesting that AMP may be useful in monitoring changes in allergic inflammation of the airways.
authors.affiliation: Dipartimento di Medicina Interna e Specialistica, University of Catania, Catania, Italy. rpolosa@hotmail.com
authors.forename: R
authors.initials: R
authors.lastname: Polosa
authors.forename: F
authors.initials: F
authors.lastname: Li Gotti
authors.forename: G
authors.initials: G
authors.lastname: Mangano
authors.forename: C
authors.initials: C
authors.lastname: Mastruzzo
authors.forename: M P
authors.initials: MP
authors.lastname: Pistorio
authors.forename: N
authors.initials: N
authors.lastname: Crimi
mesh_terms: D000249:Adenosine Monophosphate; D000328:Adult; D016535:Bronchial Hyperreactivity / Q000473:pathology / Q000503:physiopathology / Q000628:therapy; D001985:Bronchial Provocation Tests; D016085:Bronchoconstrictor Agents; D002452:Cell Count; D004311:Double-Blind Method; D005260:Female; D006801:Humans; D007114:Immunization; D007167:Immunotherapy / Q000379:methods*; D008297:Male; D016210:Methacholine Chloride; D008875:Middle Aged; D032403:Parietaria / Q000276:immunology; D011058:Pollen / Q000276:immunology; D006255:Rhinitis, Allergic, Seasonal / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D012621:Seasons; D013183:Sputum / Q000166:cytology*
chemical_list: D016085:Bronchoconstrictor Agents; D016210:Methacholine Chloride; D000249:Adenosine Monophosphate