title: In vitro antioxidant and antithrombotic activity of Hemidesmus indicus (L) R.Br.
abstract: The methanolic extract of Hemidesmus indicus (L) R.Br. (Asclepiadaceae) roots was found to inhibit lipid peroxidation and scavenge hydroxyl and superoxide radicals in vitro. The amount required for 50% inhibition of lipid peroxide formation was 217.5 micro g/ml. The concentrations needed to scavenge hydroxyl and superoxide radicals were 73.5 and 287.5 micro g/ml, respectively. The intravenous administration of this extract (5mg/kg body weight) in rabbits delayed the plasma recalcification time and enhanced the release of lipoprotein lipase enzyme significantly. The extract also inhibited ADP-induced platelet aggregation in vitro (50-250 micro g), which was comparable to commercial heparin.
authors.affiliation: Amala Cancer Research Centre, Amala Nagar, Thrissur, Kerala 680 553, India. jpadikkala@rediffmail.com
authors.forename: N K
authors.initials: NK
authors.lastname: Mary
authors.forename: C R
authors.initials: CR
authors.lastname: Achuthan
authors.forename: B H
authors.initials: BH
authors.lastname: Babu
authors.forename: J
authors.initials: J
authors.lastname: Padikkala
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology; D031102:Hemidesmus / Q000737:chemistry*; D017665:Hydroxyl Radical / Q000378:metabolism; D066298:In Vitro Techniques; D007275:Injections, Intravenous; D015227:Lipid Peroxidation / Q000187:drug effects; D008071:Lipoprotein Lipase / Q000378:metabolism; D008297:Male; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D013481:Superoxides / Q000378:metabolism; D013997:Time Factors
chemical_list: D000925:Anticoagulants; D000975:Antioxidants; D016166:Free Radical Scavengers; D010936:Plant Extracts; D010975:Platelet Aggregation Inhibitors; D013481:Superoxides; D017665:Hydroxyl Radical; D008071:Lipoprotein Lipase