High-performance liquid chromatography/inductively coupled plasma mass spectrometry with iodine-specific detection for profiling the metabolites produced in the earthworm Eisenia veneta by exposure to 2-fluoro-4-iodoaniline.
High-performance liquid chromatography/inductively coupled plasma mass spectrometry (HPLC/ICPMS) provided a rapid and specific means for profiling the iodine-containing metabolites produced by the earthworm Eisenia veneta following exposure to 2-fluoro-4-iodoaniline. Profiles were obtained, using gradient reversed-phase HPLC, from extracts of whole earthworms and from coleomic fluid with as little as 25 ng/peak of iodine detected. The use of ICPMS in this way provides a convenient means of determining the metabolic fate of iodinated compounds without the need for radiolabelled compounds.
Duckett
Catherine J
CJ
Biological Chemistry, Division of Biomedical Sciences, Faculty of Medicine, Imperial College London, Sir Alexander Fleming Building, South Kensington, London SW7 2AZ, UK.
Wilson
Ian D
ID
Walker
Heather
H
Abou-Shakra
Fadi
F
Lindon
John C
JC
Nicholson
Jeremy K
JK
D000814:Aniline Compounds / Q000493:pharmacokinetics / Q000633:toxicity*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017611:Fluorine Compounds / Q000493:pharmacokinetics / Q000633:toxicity; D007455:Iodine / Q000032:analysis / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods*; D009835:Oligochaeta / Q000187:drug effects / Q000378:metabolism*; D014020:Tissue Extracts / Q000737:chemistry / Q000378:metabolism
C477256:2-fluoro-4-iodoaniline; D000814:Aniline Compounds; D017611:Fluorine Compounds; D014020:Tissue Extracts; D007455:Iodine