Development of an inducible gene expression system for Lactobacillus sakei.
OBJECTIVE
To develop an inducible gene expression system for Lactobacillus sakei, based on the regulatory system of sakacin A production.


RESULTS
A Lactobacillus/Escherichia coli shuttle vector; pKRV3, was constructed including the signal transducing system genes of the bacteriocin sakacin A. The gusA gene fused to PsapA promoter, cloned in this vector allowed for inducible beta-glucuronidase expression in L. sakei and L. plantarum following the addition of the sakacin A inducing peptide. PsapA appeared to be a strong and tightly controlled promoter when compared with known promoters.


CONCLUSIONS
The pKRV3 system can be used as an inducible gene expression system in lactobacilli.


CONCLUSIONS
A novel, inducible gene expression system has been developed for lactic acid bacteria relevant in food fermentations.
Axelsson
L
L
MATFORSK, Norwegian Food Research Institute, Osloveien, Norway. lars.axelsson@matforsk.no
Lindstad
G
G
Naterstad
K
K
D001430:Bacteriocins / Q000096:biosynthesis; D001431:Bacteriological Techniques; D004926:Escherichia coli / Q000235:genetics; D005285:Fermentation; D005516:Food Microbiology; D015870:Gene Expression; D005798:Genes, Bacterial; D005821:Genetic Techniques; D005822:Genetic Vectors; D005966:Glucuronidase / Q000235:genetics; D007778:Lactobacillus / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic
D001430:Bacteriocins; C071456:sakacin A; D005966:Glucuronidase