[Screening method for compounds acting at super low concentrations].
At present, there are no simple and reliable methods for selection of compounds with physiological activity in super-low concentrations (SLC). The screening system with high predictability is proposed. At the beginning, experiment is carried out on infusorians--spirostoma. Their motion activity, behavior, living ability and shape are registered at concentrations of substances in a range of 10(-3)-10(-13) mol/l. If the effect is detected on spirostoma, then experiments are performed on laboratory animals or their isolated tissues. Specific deviations on injection substance in SLC are detected, depending on compounds class. Nine representatives of different classes of biologically active compounds are investigated in the work: carbofos, deltametrin, 3-quinuclidinyl benzylate, atropine, phenosan, fenazepam, dopamine receptor antagonist SCH-23390, ciprine, and hexenal. It is accepted that if compound action in SLC is observed on spirostoma, then effects in super-low doses are also detected in a whole animal.
Lebedeva
N E
NE
M.V. Lomonosov Moscow State University.
Gorbatova
E N
EN
Golovkina
T V
TV
Burlakova
E B
EB
D000818:Animals; D014151:Anti-Anxiety Agents / Q000494:pharmacology; D001285:Atropine / Q000494:pharmacology; D001552:Benzazepines / Q000494:pharmacology; D001569:Benzodiazepines; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D016798:Ciliophora / Q000187:drug effects*; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005260:Female; D006591:Hexobarbital / Q000494:pharmacology; D066298:In Vitro Techniques; D008294:Malathion / Q000494:pharmacology; D008297:Male; D051379:Mice; D009043:Motor Activity / Q000187:drug effects; D018727:Muscarinic Antagonists / Q000494:pharmacology; D009570:Nitriles; D010640:Phenothiazines / Q000494:pharmacology; D011237:Predictive Value of Tests; D011247:Pregnancy; D018873:Pregnancy Rate; D011722:Pyrethrins / Q000494:pharmacology; D011813:Quinuclidinyl Benzilate / Q000494:pharmacology; D011817:Rabbits; D051381:Rats; D015203:Reproducibility of Results; D012098:Reproduction / Q000187:drug effects
D014151:Anti-Anxiety Agents; D001552:Benzazepines; D002800:Cholinesterase Inhibitors; D018727:Muscarinic Antagonists; D009570:Nitriles; D010640:Phenothiazines; D011722:Pyrethrins; D001569:Benzodiazepines; C017180:decamethrin; C017928:phenazepam; D011813:Quinuclidinyl Benzilate; D001285:Atropine; D006591:Hexobarbital; C031637:phenothiazine; D008294:Malathion