title: Contrasting effects of interleukin-2 secretion by rat glioma cells contingent upon anatomical location: accelerated tumorigenesis in the central nervous system and complete rejection in the periphery.
abstract: Rat T9.F glioma cells were transduced with the interleukin (IL)-2 gene. Clone T9.F/IL2/#12 secreted a high level of IL-2 (15 ng/10(6) cells/48 h). Enhanced tumor progression and reduced survival was observed when T9.F/IL2/#12 cells were implanted intracranially. Subcutaneous injection of T9.F/IL2/#12 cells induced a palpable nodule, which regressed in approximately 15 days, resulting in tumor-specific protection. Lymphocytes from T9.F/IL2/#12 primed rats specifically respond to T9.F antigens but lacked cytotoxicity towards T9.F cells. Intracranial T9.F/IL2/#12 tumors were markedly infiltrated by CD4(+) and CD8(+) T cells, natural killer (NK)-T cells and myeloid progenitor cells, whereas subcutaneous T9.F/IL2/#12 tumors contained an elevated level of NK cells.
authors.affiliation: Department of Anatomy and Neurobiology, Medical College of Virginia, Virginia Commonwealth University, P.O. Box 980709 MCV Station, Richmond, VA 23298-0709, USA. mgraf@hsc.vcu.edu
authors.forename: Martin R
authors.initials: MR
authors.lastname: Graf
authors.forename: Robert M
authors.initials: RM
authors.lastname: Prins
authors.forename: Gail A
authors.initials: GA
authors.lastname: Poulsen
authors.forename: Randall E
authors.initials: RE
authors.lastname: Merchant
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000008:administration & dosage / Q000235:genetics; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D002455:Cell Division / Q000235:genetics / Q000276:immunology; D002460:Cell Line; D005260:Female; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D005910:Glioma / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D006084:Graft Rejection / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007279:Injections, Subcutaneous; D007376:Interleukin-2 / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism*; D016131:Lymphocyte Subsets / Q000276:immunology / Q000473:pathology / Q000637:transplantation; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology / Q000473:pathology; D023461:Myeloid Progenitor Cells / Q000276:immunology / Q000473:pathology; D009368:Neoplasm Transplantation; D051381:Rats; D011916:Rats, Inbred F344; D012878:Skin Neoplasms / Q000235:genetics / Q000276:immunology / Q000517:prevention & control*; D014407:Tumor Cells, Cultured
chemical_list: D000951:Antigens, Neoplasm; D007376:Interleukin-2