title: A morphological and micro-tensile bond strength evaluation of a single-bottle adhesive to caries-affected human dentine after four different caries removal techniques.
abstract: OBJECTIVE
  This study evaluated the influence of different caries removal techniques (conventional bur; chemomechanical removal/Carisolv()-MediTeam; a sonic preparation system/SonicsysMicro-Kavo and air abrasion/PrepStar-Danville Engineering) on microtensile bond strength to caries-affected human dentine.
  
  
  METHODS
  Occlusal surfaces of extracted human permanent third molars with coronal dentine caries extending approximately halfway through the dentine was ground perpendicular to the long axis of the tooth to expose a flat surface of normal dentine surrounding the carious lesion with laser fluorescence values of approximately 30 (DIAGNODent), KaVo). Carious lesions were excavated with one of the four techniques until laser fluorescence values decreased to 15 in the center of the lesions. An ethanol-based dentine adhesive (Single Bond, 3M) was used to bond composite resin (P60, 3M) to the substrate. Vertical slices (n=11/group), approximately 0.8 mm thick were made through the caries-affected portions of each tooth, perpendicular to the bonding surface. Specimens were subjected to tensile stress at a crosshead speed of 1 mm/min. Data were analyzed by Kruskal-Wallis test. SEM investigation was performed for the qualitative evaluation of resin-dentine hybridization.
  
  
  RESULTS
  The microtensile bond strengths were as follows (mean+/-SD in MPa): 6.4+/-5.3 (bur), 8.4+/-3.3 (Carisolv), 8.5+/-5.9 (Sonicsys Micro), and 8.8+/-8.8 (air abrasion). Statistical analysis did not show significant differences between any of the treatment modalities (p=0.160). Tensile fracture was cohesive within caries-affected dentine in all specimens.
  
  
  CONCLUSIONS
  The four different caries removal techniques used within this study did not influence the bond strength of the tested dentine adhesive to caries-affected human dentine.
authors.affiliation: Department of Paediatric Dentistry, Hacettepe University, Ankara, Turkey. zcehrili@hacettepe.edu.tr
authors.forename: Zafer C
authors.initials: ZC
authors.lastname: Cehreli
authors.forename: A Rüya
authors.initials: AR
authors.lastname: Yazici
authors.forename: Taner
authors.initials: T
authors.lastname: Akca
authors.forename: Gül
authors.initials: G
authors.lastname: Ozgünaltay
mesh_terms: D020906:Air Abrasion, Dental; D017438:Bisphenol A-Glycidyl Methacrylate; D003188:Composite Resins; D001840:Dental Bonding; D003731:Dental Caries / Q000628:therapy*; D003737:Dental Cavity Preparation / Q000295:instrumentation / Q000379:methods*; D003804:Dentin / Q000473:pathology / Q000648:ultrastructure; D003806:Dentin Permeability; D017220:Dentin-Bonding Agents; D005453:Fluorescence; D018698:Glutamic Acid; D006801:Humans; D007930:Leucine; D008239:Lysine; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D008963:Molar; D013718:Tensile Strength; D014464:Ultrasonic Therapy
chemical_list: C119934:Carisolv; D003188:Composite Resins; D017220:Dentin-Bonding Agents; C443708:P60 Condensible Resin Composite; C115612:single bond; D018698:Glutamic Acid; D017438:Bisphenol A-Glycidyl Methacrylate; D007930:Leucine; D008239:Lysine