Genomic effect of glucocorticoids on enzymes of intermediary metabolism in Oreochromis mossambicus.
The present study examined the effect of long-term treatment with cortisol and corticosterone on enzymes of intermediary metabolism, namely malic enzyme (ME), glucose-6-phosphate dehydrogenase (G6PDH), isocitrate dehydrogenase (ICDH), glucose 6 phosphatase (G-6-Pase), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) in Oreochromis mossambicus. Cortisol and corticosterone regulate intermediary metabolism in the liver of O. mossambicus as evidenced by changes in the activity pattern of gluconeogenic and lipogenic enzymes and amino-transferases. The long-term in vivo ip administration of glucocorticoids (GCs) suggests hyperglycemic, gluconeogenic, and antilipogenic roles of the hormones in O. mossambicus. The genomic mode of action of GCs is well established in the present study since the long-term treatment is sensitive to the action of transcription and translation inhibitors.
Sunny
Francis
F
Department of Zoology, University College, Trivandrum, India.
Jacob
Annamma
A
Oommen
Oommen V
OV
D000410:Alanine Transaminase / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D003345:Corticosterone / Q000502:physiology*; D003609:Dactinomycin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004734:Energy Metabolism / Q000187:drug effects / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D023281:Genomics; D005938:Glucocorticoids / Q000502:physiology; D005943:Gluconeogenesis / Q000187:drug effects; D005954:Glucosephosphate Dehydrogenase / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D006854:Hydrocortisone / Q000502:physiology*; D007521:Isocitrate Dehydrogenase / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008291:Malate Dehydrogenase / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D010088:Oxidoreductases / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000187:drug effects; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D011691:Puromycin / Q000494:pharmacology; D017210:Tilapia / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects
D005938:Glucocorticoids; D011500:Protein Synthesis Inhibitors; D003609:Dactinomycin; D011691:Puromycin; D010088:Oxidoreductases; D008291:Malate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D003345:Corticosterone; D006854:Hydrocortisone