title: Running interference for hedgehog signaling.
abstract: The new technology of RNA interference (RNAi) is allowing high-throughput screening of cells to examine many biological processes. Michelson discusses one such system in which the Drosophila Hedgehog (Hh) pathway was analyzed by systematic genome-wide RNAi analysis. The results not only suggest unexpected overlap in the Hh and Wingless (Wg) pathways, but also provide proof of principle for the development of high-throughput assays using RNAi techniques.
authors.affiliation: Division of Genetics, Department of Medicine, Howard Hughes Medical Institute, Brigham and Women's Hospital, 20 Shattuck Street, Boston, MA 02115, USA. michelson@rascal.med.harvard.edu
authors.forename: Alan M
authors.initials: AM
authors.lastname: Michelson
mesh_terms: D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics / Q000502:physiology*; D053823:Hedgehog Proteins; D006801:Humans; D034622:RNA Interference / Q000502:physiology*; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology*
chemical_list: D029721:Drosophila Proteins; D053823:Hedgehog Proteins; C077245:hh protein, Drosophila