Stress response in a juvenile hormone-deficient Drosophila melanogaster mutant apterous56f.
The apterous56f (ap56f) mutation leads to increases in juvenile hormone (JH) degradation levels and JH-esterase makes a greater contribution to the increase than JH-epoxide hydrolase. Dopamine levels in ap56f females, but not males, are higher than in wild-type. JH treatment of ap56f and wild-type females decreases their dopamine levels. ap56f females, but not males, produce less progeny. Survival under heat stress is dramatically decreased in ap56f females, but not males. ap56f flies show a stress reaction, as judged by changes in tyrosine decarboxylase and JH-hydrolysing activities, dopamine levels and fertility, but its intensity in the mutant females, but not males, differs significantly from wild-type. Thus, the ap56f mutation causes dramatic changes in female, but not male, metabolism and fitness.
Gruntenko
N E
NE
Institute of Cytology and Genetics, SD RAS, Novosibirsk, Russia. nataly@bionet.nsc.ru
Chentsova
N A
NA
Andreenkova
E V
EV
Bownes
M
M
Segal
D
D
Adonyeva
N V
NV
Rauschenbach
I Yu
IY
D000818:Animals; D015152:Blotting, Northern; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism*; D004851:Epoxide Hydrolases; D005260:Female; D005298:Fertility / Q000502:physiology; D015870:Gene Expression; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D018882:Heat Stress Disorders / Q000201:enzymology / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000235:genetics*; D007605:Juvenile Hormones / Q000172:deficiency* / Q000378:metabolism; D060850:LIM-Homeodomain Proteins; D008297:Male; D009154:Mutation; D014157:Transcription Factors / Q000235:genetics*; D014445:Tyrosine Decarboxylase / Q000378:metabolism
D029721:Drosophila Proteins; D018840:HSP70 Heat-Shock Proteins; D018398:Homeodomain Proteins; D007605:Juvenile Hormones; D060850:LIM-Homeodomain Proteins; D014157:Transcription Factors; C074459:ap protein, Drosophila; D002265:Carboxylic Ester Hydrolases; C038003:juvenile hormone esterase; D004851:Epoxide Hydrolases; C055698:juvenile hormone epoxide hydrolase; D014445:Tyrosine Decarboxylase; D004298:Dopamine