Nitric oxide alleviates oxidative damage in the green alga Chlorella pyrenoidosa caused by UV-B radiation.
The effect of ultraviolet-B radiation (UV-B; 280-320 nm) on induction of nitric oxide was estimated in the suspensions of green alga Chlorella pyrenoidosa with or without the NO scavenger N-acetyl-L-cysteine, and reductants such as 1,4-dithiothreitol, glutathione (reduced form), and ascorbic acid. Exogenously added sodium nitroprusside (NO donor), glutathione, 1,4-dithiothreitol, and ascorbic acid were able to prevent chlorophyll loss mediated by UV-B. Addition of NO to algal suspensions irradiated by UV-B increased the activity of catalase and superoxide dismutase but lowered the activity of phenylalanine ammonia-lyase. UV-B thus appears to be a strong inducer of NO production, exogenously added NO and reductants protecting the green alga against UV-B-induced oxidative damage.
Chen
K
K
School of Life Sciences, Lanzhou University, Lanzhou 730 000, Peoples Republic of China.
Feng
H
H
Zhang
M
M
Wang
X
X
D000111:Acetylcysteine / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology; D002374:Catalase / Q000378:metabolism; D002708:Chlorella / Q000201:enzymology / Q000378:metabolism* / Q000528:radiation effects*; D002734:Chlorophyll / Q000378:metabolism; D004229:Dithiothreitol / Q000494:pharmacology; D005978:Glutathione / Q000494:pharmacology; D009569:Nitric Oxide / Q000096:biosynthesis*; D009599:Nitroprusside / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D010650:Phenylalanine Ammonia-Lyase / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects*
D002734:Chlorophyll; D009599:Nitroprusside; D009569:Nitric Oxide; D002374:Catalase; D013482:Superoxide Dismutase; D010650:Phenylalanine Ammonia-Lyase; D005978:Glutathione; D001205:Ascorbic Acid; D004229:Dithiothreitol; D000111:Acetylcysteine