Effects of salvianolic acids on endothelial cells against damage induced by cholestane-3beta-5alpha-6beta-triol.
OBJECTIVE
To investigate the effects of salvianolic acids on human umbilical vein endothelial cells (HUVEC) against damage induced by cholestane-3beta-5alpha-6beta-triol (chol-triol).


METHODS
The viability of HUVEC was measured by MTT method. The apoptosis of HUVEC induced by chol-triol was detected by flow cytometry and TUNEL assay. The production of malondialdehyd (MDA) in HUVEC was tested by thiobarbaturic acid (TBA) assay.


RESULTS
The viability of HUVEC treated with chol-triol 100 micro mol/L decreased by 39.8% while salvianolic acids 100 micro g/ml increased by 27.9%. The apoptotic rate of HUVEC measured by PI staining increased from 6% - 8% to 17% - 20% after chol-triol treatment for 12 h. Salvianolic acids 100 micro g/ml reduced the apoptotic rate to 10% - 14% after treatment HUVEC for 1 h prior to chol-triol treatment. In another experiment, chol-triol increased the number of TUNEL-positive cells 5 times, but salvianolic acids 10 micro g/ml and 100 micro g/ml reduced the number of TUNEL-positive cells by 36.9% and 61.2%, respectively. The production of MDA in HUVEC increased by 120.7% after chol-triol treatment for 12 h. Salvianolic acids 10 micro g/ml and 100 micro g/ml also decreased the concentration of MDA by 28.7% and 39.8%, respectively.


CONCLUSIONS
Salvianolic acids has protective effect on endothelial cells against damage induced by chol-triol.
Ren
Decheng
D
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Du
Guanhua
G
Zhang
Juntian
J
D017209:Apoptosis / Q000187:drug effects; D001572:Benzofurans / Q000494:pharmacology*; D002109:Caffeic Acids / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002777:Cholestanols / Q000633:toxicity*; D002934:Cinnamates / Q000494:pharmacology*; D053630:Depsides; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D007773:Lactates / Q000494:pharmacology*; D008315:Malondialdehyde / Q000378:metabolism; D000097123:Rosmarinic Acid
D001572:Benzofurans; D002109:Caffeic Acids; D002777:Cholestanols; D002934:Cinnamates; D053630:Depsides; D007773:Lactates; D008315:Malondialdehyde; D000097123:Rosmarinic Acid; C000474:cholestane-3,5,6-triol; C066201:salvianolic acid A; C076944:salvianolic acid B