Oxidation of goat hepatic galectin-1 induces change in secondary structure.
Galectin-1 requires a reducing environment for its lectin activity and the carbohydrate binding function is destroyed in oxidizing condition. In this report we provide direct evidence that the oxidation of goat hepatic galectin-1 perturbs its carbohydrate recognition domain and this could be due to changes in secondary structure of goat hepatic galectin-1. Conformational changes in goat hepatic galectin-1 due to oxidation were investigated by absorption, fluorescence and circular dichroism measurements.
Pande
Abhay H
AH
School of Life Sciences, Devi Ahilya Vishwavidyalaya, Vigyan Bhawan, Khandwa Road Campus, Indore 452 001 (MP), India.
Gupta
Rajesh K
RK
Sumati
Hajela
Krishnan
K
D000595:Amino Acid Sequence; D000818:Animals; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry; D002942:Circular Dichroism; D005453:Fluorescence; D037483:Galectin 1 / Q000737:chemistry* / Q000378:metabolism*; D006041:Goats; D008099:Liver / Q000378:metabolism*; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D017433:Protein Structure, Secondary; D013056:Spectrophotometry, Ultraviolet
D002241:Carbohydrates; D037483:Galectin 1