title: Deiodinase type II and tissue specific mRNA alternative splicing in the Australian lungfish, Neoceratodus forsteri.
abstract: Deiodinase type II metabolises the prohormone T4 (thyroxine) into the biologically active hormone T3 (3,5,3'-triiodothyronine), at the cellular level in extrathyroidal target tissues. In juvenile lungfish, Neoceratodus forsteri, a typical deiodinase type II is present in most tissues. We have identified the full length of a 1.8 kb deiodinase type II mRNA in liver, and a truncated (1.3 kb) version in brain. Both mRNAs have two in frame UGA codons, but only the liver form has a predicted SECIS structure (form 1) in its 3'-UTR. We also report the presence of additional different length transcripts of deiodinase II mRNA, i.e., 3, 4, and 8 kb, in liver, and 8 kb in kidney, heart, and gill tissues. Expression of the longer (approximately 8 kb) transcript is very low. Real-time PCR confirmed the low expression of transcripts in all tissues, suggested by the Northern blot analysis.
authors.affiliation: Department of Biological Sciences, Macquarie University, 2109 New South Wales, Australia. msutija@rna.bio.mq.edu.au
authors.forename: Margareta
authors.initials: M
authors.lastname: Sutija
authors.forename: Terrence J
authors.initials: TJ
authors.lastname: Longhurst
authors.forename: Jean M P
authors.initials: JM
authors.lastname: Joss
mesh_terms: D017398:Alternative Splicing / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D005399:Fishes / Q000235:genetics*; D007453:Iodide Peroxidase / Q000145:classification / Q000235:genetics*; D008099:Liver / Q000378:metabolism*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D014018:Tissue Distribution
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D007453:Iodide Peroxidase