title: Characterization of Orientia tsutsugamushi isolated from wild-caught rodents and chiggers in northern Thailand.
abstract: We previously reported Orientia tsutsugamushi detection from tissue samples (kidney, liver, spleen, and whole blood) of 12 wild-caught rodents from Chiangrai Province, northern Thailand. Of the 30 chiggers individually removed from scrub typhus-infected rodents, 2 were found positive for O. tsutsugamushi. We further characterized the O. tsutsugamushi detected from these rodents and chiggers by RFLP using three different enzyme digestions. All 14 O. tsutsugamushi samples (12 from tissue samples and 2 from chiggers) showed different digestion patterns when compared to those of reference strains (Karp, Kato, and Gilliam). Interestingly, nine RFLP profiles were observed from these 14 samples suggesting the presence of high genetic diversity of O. tsutsugamushi in this area. Furthermore, one sample displayed the same RFLP pattern as that of O. tsutsugamushi mild resistant strain previously isolated from scrub typhus patient in Chiangrai. Of the two samples from positive chiggers, only one was found to have a similar RFLP pattern to that of its host rodent. DNA sequencing of the entire 56 kDa genome of these O. tsutsugamushi samples is in progress.
authors.affiliation: Department of Entomology, United States Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok 10400, Thailand.
authors.forename: B
authors.initials: B
authors.lastname: Khuntirat
authors.forename: K
authors.initials: K
authors.lastname: Lerdthusnee
authors.forename: W
authors.initials: W
authors.lastname: Leepitakrat
authors.forename: A
authors.initials: A
authors.lastname: Kengluecha
authors.forename: K
authors.initials: K
authors.lastname: Wongkalasin
authors.forename: T
authors.initials: T
authors.lastname: Monkanna
authors.forename: S
authors.initials: S
authors.lastname: Mungviriya
authors.forename: J W
authors.initials: JW
authors.lastname: Jones
authors.forename: R E
authors.initials: RE
authors.lastname: Coleman
mesh_terms: D000818:Animals; D000835:Animals, Wild; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D007668:Kidney / Q000382:microbiology; D008099:Liver / Q000382:microbiology; D012285:Orientia tsutsugamushi / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012377:Rodentia / Q000382:microbiology*; D013154:Spleen / Q000382:microbiology; D013785:Thailand; D014324:Trombiculidae / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial