title: Expression of deleted in malignant brain tumor-1 (DMBT1) molecule in biliary epithelium is augmented in hepatolithiasis: possible participation in lithogenesis.
abstract: Deleted in malignant brain tumor-1 (DMBT1) is a mucin-like molecule participating in mucosal immune defense. Given that bovine gallbladder mucin, which accelerates cholesterol crystallization, is a DMBT1 homolog, DMBT1 expression was examined immunohistochemically in biliary epithelial cells in livers with hepatolithiasis (N = 25), primary sclerosing cholangitis (N = 7), large bile duct obstruction (N = 12), and control normal livers (N = 10). DMBT1 protein was determined in the hepatic bile samples of hepatolithiasis (N = 12) and other hepatobiliary diseases (N = 8) by immunoblot. While DMBT1 was faintly expressed in normal livers (20%), it was significantly augmented in hepatolithiasis (76%) (P < 0.05). DMBT1 was mildly expressed in primary sclerosing cholangitis and large bile duct obstruction. DMBT1 protein was detected frequently in hepatic bile samples of hepatolithiasis (50%) (P < 0.05), but in the other bile samples. The percentage of cholesterol in intrahepatic calculi was significantly higher in the patients with DMBT1-positive bile. Augmented expression and secretion of DMBT1 in intrahepatic large bile ducts in hepatolithiasis suggests its role in lithogenesis.
authors.affiliation: Department of Human Pathology, Kanazawa University Graduate School of Medicine, Kanazawa, 920-8640, Japan.
authors.forename: Motoko
authors.initials: M
authors.lastname: Sasaki
authors.forename: Shiu-Feng
authors.initials: SF
authors.lastname: Huang
authors.forename: Miin-Fu
authors.initials: MF
authors.lastname: Chen
authors.forename: Yi-Yin
authors.initials: YY
authors.lastname: Jan
authors.forename: Ta-Sen
authors.initials: TS
authors.lastname: Yeh
authors.forename: Akira
authors.initials: A
authors.lastname: Ishikawa
authors.forename: Jan
authors.initials: J
authors.lastname: Mollenhauer
authors.forename: Annemarie
authors.initials: A
authors.lastname: Poustka
authors.forename: Koichi
authors.initials: K
authors.lastname: Tsuneyama
authors.forename: Yuji
authors.initials: Y
authors.lastname: Nimura
authors.forename: Koji
authors.initials: K
authors.lastname: Oda
authors.forename: Yasuni
authors.initials: Y
authors.lastname: Nakanuma
mesh_terms: D000373:Agglutinins; D001652:Bile Ducts / Q000378:metabolism* / Q000503:physiopathology; D001653:Bile Ducts, Intrahepatic; D002135:Calcium-Binding Proteins; D002769:Cholelithiasis / Q000737:chemistry / Q000378:metabolism* / Q000503:physiopathology; D002784:Cholesterol / Q000032:analysis; D004268:DNA-Binding Proteins; D004848:Epithelium; D006801:Humans; D012333:RNA, Messenger / Q000096:biosynthesis; D011956:Receptors, Cell Surface / Q000096:biosynthesis*; D025521:Tumor Suppressor Proteins
chemical_list: D000373:Agglutinins; D002135:Calcium-Binding Proteins; C108174:DMBT1 protein, human; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D025521:Tumor Suppressor Proteins; D002784:Cholesterol