title: Proteomics approaches to uncover the repertoire of circulating biomarkers for breast cancer.
abstract: There is substantial interest in applying proteomics to cancer biomarker discovery. Proteomics is particularly suited for profiling biological fluids and uncovering circulating markers and is currently being applied to the identification of novel biomarkers for breast cancer. Novel proteomic approaches include direct profiling of serum using mass spectrometry, application of a variety of strategies to harness immunity for cancer diagnosis, protein tagging to capture subproteomes rich in diagnostic markers, the use of protein microarrays, and the use of multi-dimensional liquid protein separation technologies for comprehensive profiling of serum and other biological fluids. It is likely that the application of a wide range of proteomics tools to breast cancer will yield a panel of markers that have utility for breast cancer diagnosis.
authors.affiliation: Department of Pediatrics, University of Michigan, Ann Arbor, Michigan 48109-0656, USA.
authors.forename: Bong Kyung
authors.initials: BK
authors.lastname: Shin
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Samir
authors.initials: S
authors.lastname: Hanash
mesh_terms: D001323:Autoantibodies / Q000276:immunology; D014408:Biomarkers, Tumor; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000097:blood* / Q000378:metabolism; D006801:Humans; D013058:Mass Spectrometry; D040081:Protein Array Analysis / Q000379:methods; D020543:Proteome; D016622:Silver Staining
chemical_list: D001323:Autoantibodies; D014408:Biomarkers, Tumor; D020543:Proteome