title: Fluorescence-enhanced optical imaging in large tissue volumes using a gain-modulated ICCD camera.
abstract: A novel image-intensified charge-coupled device (ICCD) imaging system has been developed to perform 3D fluorescence tomographic imaging in the frequency-domain using near-infrared contrast agents. The imager is unique since it (i) employs a large tissue-mimicking phantom, which is shaped and sized to resemble a female breast and part of the extended chest-wall region, and (ii) enables rapid data acquisition in the frequency-domain by using a gain-modulated ICCD camera. Diffusion model predictions are compared to experimental measurements using two different referencing schemes under two different experimental conditions of perfect and imperfect uptake of fluorescent agent into a target. From these experimental measurements, three-dimensional images of fluorescent absorption were reconstructed using a computationally efficient variant of the approximate extended Kalman filter algorithm. The current work represents the first time that 3D fluorescence-enhanced optical tomographic reconstructions have been achieved from experimental measurements of the time-dependent light propagation on a clinically relevant breast-shaped tissue phantom using a gain-modulated ICCD camera.
authors.affiliation: Department of Chemical Engineering, Texas A&M University, College Station, TX 77843-3122, USA.
authors.forename: Anuradha
authors.initials: A
authors.lastname: Godavarty
authors.forename: Margaret J
authors.initials: MJ
authors.lastname: Eppstein
authors.forename: Chaoyang
authors.initials: C
authors.lastname: Zhang
authors.forename: Sangeeta
authors.initials: S
authors.lastname: Theru
authors.forename: Alan B
authors.initials: AB
authors.lastname: Thompson
authors.forename: Michael
authors.initials: M
authors.lastname: Gurfinkel
authors.forename: Eva M
authors.initials: EM
authors.lastname: Sevick-Muraca
mesh_terms: D055592:Biophysical Phenomena; D001703:Biophysics; D001940:Breast / Q000033:anatomy & histology; D001941:Breast Diseases / Q000175:diagnosis; D004867:Equipment Design; D005260:Female; D005453:Fluorescence; D006801:Humans; D007091:Image Processing, Computer-Assisted; D055095:Optics and Photonics / Q000295:instrumentation; D019047:Phantoms, Imaging; D014054:Tomography / Q000295:instrumentation*