Characterization of two new HLA-B alleles by sequence-based typing: HLA-B*0817 and HLA-B*1311.
In this brief communication we report the characterization of two new HLA-B variants officially named HLA-B*0817 and HLA-B*1311. The HLA-B*0817 allele was identified in a Caucasoid male candidate for renal transplantation in the North Italy Transplant program. The nucleotidic sequence of exons 2, 3 and 4 of this novel allele is identical to that of HLA-B*0804 except for three point mutations in exon 2: from A to G at position 259, from C to G at position 261 and from G to A at position 302. These mutations are responsible for two aminoacidic substitutions [Asn (r) Glu, codon 63, and Ser (r) Asn, codon 77]. HLA-B*1311 was found in a volunteer donor belonging to National Marrow Donor Program(R). This new variant is identical to that of HLA-B*1301 except for three nucleotide substitutions at positions 353, 355 and 369 leading to two aminoacidic variations from Ile to Thr at codon 94 and from Ile to Leu at codon 95 and a silent mutation at codon 99.
Longhi
E
E
Centro Trasfusionale e di Immunologia dei Trapianti, Ospedale Maggiore Policlinico IRCCS, Milan, Italy.
Frison
S
S
Espaolas de Arias
A
A
Bianchi
P
P
Ferrioli
G
G
Poli
F
F
Scalamogna
M
M
D000483:Alleles; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001483:Base Sequence; D003062:Codon; D005091:Exons; D014644:Genetic Variation; D015235:HLA-B Antigens / Q000145:classification / Q000235:genetics* / Q000276:immunology; D006650:Histocompatibility Testing; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology; D008297:Male; D008969:Molecular Sequence Data; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000379:methods; D044465:White People / Q000235:genetics
D003062:Codon; D015235:HLA-B Antigens