title: Microarray transcription analysis of clinical Staphylococcus aureus isolates resistant to vancomycin.
abstract: The transcriptomes of vancomycin intermediate-resistance Staphylococcus aureus (VISA) clinical isolates HIP5827 and Mu50 (MIC = 8 micro g/ml) were compared to those of highly vancomycin-resistant S. aureus (VRSA; MIC = 32 micro g/ml) passage derivatives by microarray. There were 35 genes with increased transcription and 16 genes with decreased transcription in common between the two VRSAs compared to those of their VISA parents. Of the 35 genes with increased transcription, 15 involved purine biosynthesis or transport, and the regulator (purR) of the major purine biosynthetic operon (purE-purD) was mutant. We hypothesize that increased energy (ATP) is required to generate the thicker cell walls that characterize resistant mutants.
authors.affiliation: Department of Medicine, Medical College of Virginia at Virginia Commonwealth University and the Hunter Holmes McGuire Veterans Affairs Medical Center, Richmond, Virginia, USA.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Mongodin
authors.forename: Jon
authors.initials: J
authors.lastname: Finan
authors.forename: Michael W
authors.initials: MW
authors.lastname: Climo
authors.forename: Adriana
authors.initials: A
authors.lastname: Rosato
authors.forename: Steven
authors.initials: S
authors.lastname: Gill
authors.forename: Gordon L
authors.initials: GL
authors.lastname: Archer
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D014158:Transcription, Genetic; D014640:Vancomycin / Q000494:pharmacology; D020713:Vancomycin Resistance
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D014640:Vancomycin