Regulation of hemopexin transcription by calcium ionophores and phorbol ester in hepatoma cells.
Hemopexin (Hx) is an acute-phase hepatic protein, whose transcription is upregulated by IL-6. The transcription rate of Hx was found to be increased 11-fold by the calcium ionophore A23187, 25-fold by the calcium ionophore ionomycin, and 4-5-fold by phorbol 12-myristate 13-acetate (PMA) in serum-starved H4IIE rat hepatoma cells. Insulin did not affect the transcription rate of Hx. These findings are consistent with involvement of intracellular calcium concentrations and activation of protein kinase C (PKC) action in the regulation of Hx.
Stred
Susan E
SE
Cell and Molecular Biology Program and Department of Pediatrics, SUNY Upstate Medical University, Syracuse, NY 13210, USA.
Cote
Deborah
D
Weinstock
Ruth S
RS
Messina
Joseph L
JL
D000818:Animals; D002118:Calcium / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D045744:Cell Line, Tumor; D005786:Gene Expression Regulation / Q000187:drug effects; D006466:Hemopexin / Q000235:genetics*; D007328:Insulin / Q000494:pharmacology; D007476:Ionophores / Q000494:pharmacology; D007700:Kinetics; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects
D007328:Insulin; D007476:Ionophores; D006466:Hemopexin; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium