title: Crystallization and preliminary X-ray crystallographic studies on the fungal immunomodulatory protein Fve from the golden needle mushroom (Flammulina velutipes).
abstract: The fungal immunomodulatory protein from the edible golden needle mushroom (Flammulina velutipes), designated Fve, is a single polypeptide consisting of 114 amino-acid residues. It is believed to trigger the mitogenic proliferation of T lymphocytes and Th1 cytokine production. Here, it is demonstrated that Fve forms a homodimer in nature. In order to understand the relationship between its structure and function, Fve was crystallized using the hanging-drop method; the protein formed well diffracting crystals within 3-5 d in 2.5% PEG 400, 2.0 M ammonium sulfate and 0.1 M Tris base buffer pH 8.5. The space group of the Fve crystals is either P4(3)2(1)2 or P4(1)2(1)2, with unit-cell parameters a = b = 96.92, c = 61.42 A. The crystal contains two molecules per asymmetric unit and diffracts to 1.4 A resolution when exposed to synchrotron radiation.
authors.affiliation: Department of Pediatrics, Faculty of Medicine, National University of Singapore, 5 Lower Kent Ridge Road, Singapore 119074, Singapore.
authors.forename: See Voon
authors.initials: SV
authors.lastname: Seow
authors.forename: I-Chun
authors.initials: IC
authors.lastname: Kuo
authors.forename: Palasingam
authors.initials: P
authors.lastname: Paaventhan
authors.forename: Prasanna R
authors.initials: PR
authors.lastname: Kolatkar
authors.forename: Kaw Yan
authors.initials: KY
authors.lastname: Chua
mesh_terms: D000363:Agaricales / Q000378:metabolism*; D000645:Ammonium Sulfate / Q000737:chemistry; D002850:Chromatography, Gel; D018360:Crystallography, X-Ray; D019281:Dimerization; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D012997:Solvents / Q000737:chemistry
chemical_list: D005656:Fungal Proteins; D037102:Lectins; D012997:Solvents; D011092:Polyethylene Glycols; D000645:Ammonium Sulfate