Triosephosphates are toxic to superoxide dismutase-deficient Escherichia coli.
Increase in the production of triosephosphates has been considered an important factor leading to diabetic complications. It might be expected that like the other short chain monosaccharides, triosephosphates autoxidize producing superoxide radical and alpha,beta-diketones. Since superoxide can also initiate the oxidation of short chain sugars, free radical chain reactions are possible. If such reactions occur in vivo, triosephosphates would be more deleterious to cells lacking superoxide dismutase (SOD) than to normal cells. Here we demonstrate that triosephosphates kill a SOD-deficient Escherichia coli mutant much more than the parental, SOD-proficient strain. The effect is oxygen-dependent and is partially suppressed by aminoguanidine. Increased production of superoxide and diketones appeared to be the cause of triosephosphates toxicity.
Benov
Ludmil
L
Department of Biochemistry, Faculty of Medicine, Kuwait University, PO Box 24923 Safat, 13110, Kuwait. lbenov@hsc.kuniv.edu.kw
Beema
Anees F
AF
Sequeira
Fatima
F
D000693:Anaerobiosis; D004099:Dihydroxyacetone Phosphate / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D005988:Glyceric Acids / Q000494:pharmacology; D006146:Guanidines; D009152:Mutagenicity Tests; D009943:Organophosphorus Compounds / Q000737:chemistry / Q000494:pharmacology*; D010100:Oxygen; D013482:Superoxide Dismutase / Q000172:deficiency / Q000502:physiology*; D013481:Superoxides / Q000737:chemistry / Q000378:metabolism
D005988:Glyceric Acids; D006146:Guanidines; D009943:Organophosphorus Compounds; D013481:Superoxides; D004099:Dihydroxyacetone Phosphate; C005156:3-phosphoglycerate; D013482:Superoxide Dismutase; D010100:Oxygen; C004479:pimagedine