Ex vivo biocompatibility of a new beta2-microglobulin hemoperfusion polymer.
Beta2-microglobulin (beta2-m) is an 11.8 kD protein that is excreted by the kidneys. In renal insufficiency, it accumulates in the body and can result in AB amyloidosis with bone and joint destruction. Four modifications of a new beta2-m adsorbent material were tested for biocompatibility with human whole blood. 500 ml of heparinized blood from healthy human donors was perfused ex vivo through minicolumns (adsorber beads: divinylbenzene with different biocompatible coatings) in the single-pass mode. Blood samples were taken from the antecubital vein before and at the column outlet during the 50 min test runs. Red and white cell counts remained virtually constant. No signs of hemolysis could be detected. Thrombogenicity of the columns was low as shown by the insignificant platelet loss, only slight platelet activation and moderate thrombin-antithrombin formation. There was no activation of leukocytes nor monocytes. Complement and bradykinin activation was minimal. Electrolyte concentrations and pH remained essentially constant. In conclusion, this new beta2-m adsorbent material exhibited favorable biocompatibility features in our ex vivo model and is thus a promising candidate for future clinical beta2-m hemoperfusion studies in patients.
Wendler
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Nephrology Division, Department of Internal Medicine I, Klinikum Grosshadern, University of Munich, Munich, Germany.
Duhr
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Bosch
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D000990:Antithrombin III; D020099:Coated Materials, Biocompatible / Q000009:adverse effects / Q000627:therapeutic use*; D015926:Complement C3a / Q000032:analysis; D006402:Hematologic Diseases / Q000209:etiology; D006801:Humans; D007688:Kidneys, Artificial; D010447:Peptide Hydrolases / Q000097:blood; D011137:Polystyrenes / Q000009:adverse effects / Q000627:therapeutic use*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis; D001613:beta 2-Microglobulin / Q000493:pharmacokinetics*
D020099:Coated Materials, Biocompatible; D011137:Polystyrenes; D014409:Tumor Necrosis Factor-alpha; C046193:antithrombin III-protease complex; D001613:beta 2-Microglobulin; C432006:divinylbenzene-polystyrene copolymer; D015926:Complement C3a; D000990:Antithrombin III; D010447:Peptide Hydrolases