NADPH-diaphorase staining reveals new types of interneurons in human putamen.
Neurons in the human striatum have been divided into five or seven different types, respectively. To further characterize these interneurons, we investigated the putamen of five brains by means of NADPH-diaphorase staining and compared our results to previous classifications in man. The NADPH-diaphorase method is selective for nitric oxide synthase (NOS); in the human striatum, predominantly interneurons were stained. NADPH-diaphorase positive neurons were then further examined. They showed clear morphological differences and could be classified into 12 different types, which only partially corresponded to previously described neuron types. Thus, we suggest at least three novel types of neostriatal interneurons. Furthermore, a special class of large neurons thought to be efferent in nature, stained NOS-positive.
Johannes
Silvia
S
Laboratory of Neurobiology, Department of Psychiatry and Psychotherapy, Julius-Maximilians-University Würzburg, Füchsleinstr. 15, D-97080, Würzburg, Germany.
Reif
Andreas
A
Senitz
Dieter
D
Riederer
Peter
P
Lauer
Martin
M
D000328:Adult; D001344:Autopsy; D005260:Female; D006801:Humans; D007395:Interneurons / Q000145:classification*; D008297:Male; D008875:Middle Aged; D009252:NADPH Dehydrogenase; D019001:Nitric Oxide Synthase / Q000032:analysis; D011699:Putamen / Q000737:chemistry / Q000166:cytology*; D013194:Staining and Labeling
D019001:Nitric Oxide Synthase; D009252:NADPH Dehydrogenase