title: An improved method for the purification and refolding of r56-kDa proteins from Gilliam and Kato strains of Orientia tsutsugamushi.
abstract: The immunodominant 56kDa outer membrane antigens from Orientia tsutsugamushi Kato and Gilliam strains were expressed as inclusion bodies (IBs) in E. coli. The IBs were purified and properly refolded with modifications of a previous procedure used for the production of Karp strain r56 antigen. A mixture of these three r56 proteins exhibited both high sensitivity and specificity for detection of O. tsutsugamushi antibodies by ELISA.
authors.affiliation: Rickettsial Diseases Program, Infectious Diseases Directorate, Naval Medical Research Center, Silver Spring, Maryland 20910, USA.
authors.forename: Qing
authors.initials: Q
authors.lastname: Yang
authors.forename: Wei-Mei
authors.initials: WM
authors.lastname: Ching
authors.forename: Ju
authors.initials: J
authors.lastname: Jiang
authors.forename: Leslie
authors.initials: L
authors.lastname: Lousteau
authors.forename: Allen L
authors.initials: AL
authors.lastname: Richards
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D008970:Molecular Weight; D012285:Orientia tsutsugamushi / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D011489:Protein Denaturation; D017510:Protein Folding; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D011994:Recombinant Proteins