[Prevalence of hepatitis B virus core promoter mutant isolated from asymptomatic carriers from areas with higher and lower incidence of hepatocellular carcinoma in Guangxi].
OBJECTIVE
To understand the prevalence of HBV core promoter mutant (T1762 A1764 mutant) isolated from asymptomatic carriers from areas with higher and lower incidence of hepatocellular carcinoma (HCC) in Guangxi.


METHODS
A nested polymerase chain reaction (nPCR) was used for amplification of HBV DNA core promoter in sera, and then HBV DNA nPCR products were sequenced by direct sequencing.


RESULTS
The results show that 50.6% (39/77) of all HBV asymptomatic carriers were positive for HBV DNA HBV DNA positive rates of the samples from HCC higher incidence area, Longan County, and from lower incidence area, Guilin city were 55.6% (20/36) and 46.3% (19/41), respectively. HBV core promoter mutants could be seen in 35% in Longan positive samples and 47.4% in Guilin. The common mutations in both regions were all double mutations (nt 1,762 A-->T; nt 1,764 G-->A), accounting for 25% and 21%, respectively. The difference of the double mutant between Longan County and Guilin city was not significant (P>0.05).


CONCLUSIONS
These data implicated that the prevalence of HBV core promoter mutant isolated from asymptomatic carriers may not be correlated with the incidence of HCC in Guangxi.
Fang
Zhong-liao
ZL
Center for Diseases Prevention and Control of Guangxi, Nanning 530021, China.
Zhuang
Hui
H
Wang
You-chun
YC
Ge
Xian-min
XM
Yang
Jin-ye
JY
Gong
Jian
J
Li
Rong-cheng
RC
Ling
Roger
R
Harrison
Tim J
TJ
D000293:Adolescent; D000328:Adult; D006528:Carcinoma, Hepatocellular / Q000821:virology; D002353:Carrier State / Q000821:virology; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D006509:Hepatitis B / Q000821:virology*; D006512:Hepatitis B Core Antigens / Q000235:genetics*; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D008113:Liver Neoplasms / Q000821:virology*; D008297:Male; D008875:Middle Aged; D017354:Point Mutation; D011401:Promoter Regions, Genetic / Q000235:genetics
D004279:DNA, Viral; D006512:Hepatitis B Core Antigens