title: Rapid establishment of long-term culture-initiating cells of donor origin after nonmyeloablative allogeneic hematopoietic stem-cell transplantation, and significant prognostic impact of donor T-cell chimerism on stable engraftment and progression-free survival.
abstract: BACKGROUND
  Nonmyeloablative allogeneic hematopoietic stem-cell transplantation (NST) allows establishment of donor hematopoiesis without eradication of recipient stem cells by chemoradiotherapy. Quantification of donor chimerism may predict graft failure and relapse.
  
  
  METHODS
  We quantified donor long-term culture-initiating cells (LTC-IC) in nine patients during the early phase after NST and lineage-specific donor cells of myeloid (CD33+, CD34+, granulocytes) and lymphoid lineage (CD3+, CD4+, CD8+, CD56+) in 38 patients with a median follow-up of 40 weeks after NST. Conditioning therapy consisted of fludarabine 90 mg/m2 followed by total body irradiation of 2 Gy.
  
  
  RESULTS
  Only rapid establishment of donor T-cell chimerism was essential for stable donor engraftment. Patients with less than 90% of donor T cells 4 weeks after NST had a significantly higher risk of relapse, graft rejection, or both (14 of 18 patients) than patients with donor T-cell chimerism of 90% and higher (3 of 20 patients). Although conditioning therapy was nonmyeloablative, a significant decrease of repopulating stem cells defined as LTC-IC was seen after 2 weeks followed by rapid recovery of LTC-IC to pretransplant values. Interestingly, all LTC-IC were from donor origin 2 and 4 weeks after NST, but rapid establishment of donor LTC-IC was not predictive for progression-free survival.
  
  
  CONCLUSIONS
  Rapid establishment of lymphoid but not myeloid donor chimerism is a prognostic factor for stable donor engraftment after NST. It seems that an immunologic shield of alloreactive donor T cells is essential for early hematopoietic progenitors.
authors.affiliation: Department of Internal Medicine I, Bone Marrow Transplantation, University of Vienna, Austria. felix.keil@akh-wien.ac.at
authors.forename: Felix
authors.initials: F
authors.lastname: Keil
authors.forename: Erika
authors.initials: E
authors.lastname: Prinz
authors.forename: Karin
authors.initials: K
authors.lastname: Moser
authors.forename: Christine
authors.initials: C
authors.lastname: Mannhalter
authors.forename: Peter
authors.initials: P
authors.lastname: Kalhs
authors.forename: Nina
authors.initials: N
authors.lastname: Worel
authors.forename: Werner
authors.initials: W
authors.lastname: Rabitsch
authors.forename: Axel
authors.initials: A
authors.lastname: Schulenburg
authors.forename: Margit
authors.initials: M
authors.lastname: Mitterbauer
authors.forename: Hildegard
authors.initials: H
authors.lastname: Greinix
mesh_terms: D000017:ABO Blood-Group System; D000293:Adolescent; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000097:blood; D001787:Blood Group Incompatibility; D018929:Cell Culture Techniques / Q000379:methods; D018572:Disease-Free Survival; D005260:Female; D006086:Graft vs Host Disease / Q000517:prevention & control; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D017404:In Situ Hybridization, Fluorescence; D007938:Leukemia / Q000628:therapy; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000628:therapy; D011379:Prognosis; D033581:Stem Cell Transplantation / Q000379:methods*; D013601:T-Lymphocytes / Q000276:immunology*; D014019:Tissue Donors; D018183:Transplantation Chimera / Q000276:immunology*; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous; D016896:Treatment Outcome
chemical_list: D000017:ABO Blood-Group System; D015703:Antigens, CD; D007166:Immunosuppressive Agents