title: Screening of RNA molecules inhibiting human acetylcholinesterase by virtue of systematic evolution of ligands by exponential enrichment.
abstract: OBJECTIVE
  To acquire the specific RNA aptamers inhibiting human red blood cell (RBC) acetylcholinesterase (AChE).
  
  
  METHODS
  Systematic evolution of ligands by exponential enrichment (SELEX) aptamer against human red blood cell membrane AChE was selected by microtiter plate method in vitro. The specifity binding to AChE was determined by gel mobility shift analysis. Microcolorispectrophotometric method was used to measure the activity of AChE.
  
  
  RESULTS
  The aptamers to human RBC AChE were identified by 9 reiterative rounds. At the same concentration (2.25 micromol/L), the aptamers did not bind to the recombinant human butyrylcholinesterase (rhBChE) but specifically bound to human RBC AChE and inhibited the enzyme activity.
  
  
  CONCLUSIONS
  It is an effective way to isolate the specific AChE inhibitor from the vast oligonucleotide combinatorial library by virtue of SELEX.
authors.affiliation: Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Xing-Mei
authors.initials: XM
authors.lastname: Zhang
authors.forename: Ning-Sheng
authors.initials: NS
authors.lastname: Shao
authors.forename: Mu-Gen
authors.initials: MG
authors.lastname: Chi
authors.forename: Man-Ji
authors.initials: MJ
authors.lastname: Sun
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D001665:Binding Sites; D002800:Cholinesterase Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D020650:Combinatorial Chemistry Techniques; D004910:Erythrocyte Membrane / Q000737:chemistry; D006801:Humans; D008024:Ligands; D012313:RNA / Q000302:isolation & purification* / Q000494:pharmacology; D016601:RNA-Binding Proteins / Q000378:metabolism
chemical_list: D002800:Cholinesterase Inhibitors; D008024:Ligands; D016601:RNA-Binding Proteins; D012313:RNA; D000110:Acetylcholinesterase