Effect of Holotrichia diomphalia larvae on liver fibrosis and hepatotoxicity in rats.
Holotrichia diomphalia larvae, one of the most widely used Korean folk medicinal preparations, have long been used for the treatment of chronic liver cirrhosis. The present study was undertaken to clarify whether extract of Holotrichia diomphalia larvae could prevent acute liver damage and liver fibrosis in rats. A single administration of Holotrichia diomphalia protected rats from acute liver damage induced by carbon tetrachloride (200 micro l/kg, i.p.) and beta-D-galactosamine (600mg/kg, i.p.). This was evidenced by the lowered serum aminotransferase (ALT, AST) activities in rats treated with Holotrichia diomphalia. The hepatic cirrhosis was induced by 28 days of bile duct ligation/scission in rats. The four-week treatment with Holotrichia diomphalia reduced the serum ALT, AST, alkaline phosphatase activities, and hydroxyproline content in the liver and improved the histological appearance of the liver sections. The present results led us to conclude that Holotrichia diomphalia larvae can reduce the degree of hepatocellular damage and may become a promising antifibrotic agent for liver fibrosis/cirrhosis.
Oh
Woo Yong
WY
College of Pharmacy, Sungkyunkwan University, 300 Chonchon-dong, Changan-gu, 440-746, Kyunggi-do, Suwon, South Korea.
Pyo
Suhkneung
S
Lee
Kang-Ro
KR
Lee
Bum-Koo
BK
Shin
Dae-Hee
DH
Cho
Sung Ig
SI
Lee
Sun-Mee
SM
D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002252:Carbon Tetrachloride Poisoning / Q000188:drug therapy* / Q000473:pathology; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology / Q000517:prevention & control*; D001517:Coleoptera / Q000737:chemistry*; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D006909:Hydroxyproline / Q000378:metabolism; D007814:Larva / Q000737:chemistry; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008106:Liver Cirrhosis, Experimental / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D010936:Plant Extracts / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D014020:Tissue Extracts / Q000627:therapeutic use
D010936:Plant Extracts; D014020:Tissue Extracts; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D006909:Hydroxyproline