Tandem transcription and translation regulatory sensing of uncharged tryptophan tRNA.
The Bacillus subtilis AT (anti-TRAP) protein inhibits the regulatory protein TRAP (trp RNA-binding attenuation protein), thereby eliminating transcription termination in the leader region of the trp operon. Transcription of the AT operon is activated by uncharged tryptophan transfer RNA (tRNATrp). Here we show that translation of AT also is regulated by uncharged tRNATrp. A 10-residue coding region containing three consecutive tryptophan codons is located immediately preceding the AT structural gene. Completion of translation of this coding region inhibits AT synthesis, whereas incomplete translation increases AT production. Tandem sensing of uncharged tRNATrp therefore regulates synthesis of AT, which in turn regulates TRAP's ability to inhibit trp operon expression.
Chen
Guangnan
G
Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA.
Yanofsky
Charles
C
D000878:Anthranilate Synthase / Q000378:metabolism; D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D003062:Codon; D018387:Codon, Initiator; D018388:Codon, Terminator; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D009690:Nucleic Acid Conformation; D009876:Operon; D014176:Protein Biosynthesis; D021382:Protein Sorting Signals / Q000235:genetics; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012364:RNA, Transfer, Trp / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D014364:Tryptophan / Q000096:biosynthesis / Q000235:genetics*
D001426:Bacterial Proteins; D003062:Codon; D018387:Codon, Initiator; D018388:Codon, Terminator; C079092:MtrB protein, Bacteria; D021382:Protein Sorting Signals; D012329:RNA, Bacterial; D012364:RNA, Transfer, Trp; D016601:RNA-Binding Proteins; D014157:Transcription Factors; C435924:yczA protein, Bacillus subtilis; D014364:Tryptophan; D000878:Anthranilate Synthase