title: [Detection of hantaan virus from gamasid mite and chigger mite by molecular biological methods].
abstract: OBJECTIVE
  To study the proliferation and location of hantaan virus (HV) in gamasid mites and chigger mites.
  
  
  METHODS
  HV RNA in gamasid mites and chigger mites were detected by reverse transcription, polymerase chain reaction (RT- PCR) and in situ hybridization.
  
  
  RESULTS
  The smallest quantity of mite from which HV RNA could be detected was 5 mites group. The titers of -and proliferated in mites HV RNA could be found in ovary cells and dug cells of gamasid mites and chigger mites by in situ hybridization.
  
  
  CONCLUSIONS
  The results showed that HV could be trans-stadially transmitted and proliferated in mites, and HV always located in ovary and dug organs of mites. These results provide direct evidence at molecular level for the role of gamasid mites and chigger mites as vectors in transmission of HV.
authors.affiliation: Institute of Military Medicine, Nanjing Command of PLA, Nanjing 210002, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jin
authors.initials: J
authors.lastname: Zhu
authors.forename: Xiao-zhao
authors.initials: XZ
authors.lastname: Deng
authors.forename: Guang-hua
authors.initials: GH
authors.lastname: Wu
authors.forename: Jing-jun
authors.initials: JJ
authors.lastname: Wang
authors.forename: Jia-ju
authors.initials: JJ
authors.lastname: Zhang
authors.forename: Ai-hua
authors.initials: AH
authors.lastname: Xing
authors.forename: Jian-wei
authors.initials: JW
authors.lastname: Wu
mesh_terms: D000818:Animals; D001096:Arachnid Vectors; D002522:Chlorocebus aethiops; D005260:Female; D018063:Hantaan virus / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D006801:Humans; D017403:In Situ Hybridization; D007814:Larva / Q000821:virology; D008925:Mites / Q000821:virology*; D009758:Nymph / Q000821:virology; D010053:Ovary; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012367:RNA, Viral