In situ localization of cell wall alpha-1,3-glucan in the fission yeast Schizosaccharomyces pombe.
The yeast cell walls of the budding yeast Saccharomyces cerevisiae are well studied and the results show the existence of a framework composed of beta-1,3-glucan. It is reported that the cell wall of the fission yeast Schizosaccharomyces pombe has different components and our analysis by 13C-nuclear magnetic resonance (NMR) spectroscopy also showed there is alpha-1,3-glucan in its cell wall. To refine our understanding of the architecture of the yeast cell wall, we re-examined the cell wall glucans of S. pombe by NMR spectroscopy and prepared antibody against alpha-1,3-glucan, which is a characteristic component of this yeast. By the competitive enzyme-linked immunosorbent assay (ELISA) system, specificity of the antibody was restricted to alpha-1,3-glucan, which did not take a highly ordered structure. We analysed the localization of the cell wall glucans by immunoelectron microscopy. Transmission electron microscope (TEM) images showed that most of the alpha-1,3-glucan was along the cell membrane and appeared to enclose the cytoplasm, supporting previous reports that this glucan is synthesized on the cell membrane.
Sugawara
Tomoko
T
Sato
Mamiko
M
Takagi
Tomoko
T
Kamasaki
Tomoko
T
Ohno
Naohito
N
Osumi
Masako
M
D000911:Antibodies, Monoclonal; D002473:Cell Wall / Q000737:chemistry / Q000648:ultrastructure; D005615:Freezing; D005936:Glucans / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008854:Microscopy, Electron; D011312:Pressure; D012568:Schizosaccharomyces / Q000737:chemistry* / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D005936:Glucans; C045788:alpha-1,3-glucan