Biochemical function of msl5 (pks8 plus pks17) in Mycobacterium tuberculosis H37Rv: biosynthesis of monomethyl branched unsaturated fatty acids.
We show that the disruption of one of the mycocerosic acid synthase (mas)-like genes, msl5 (pks8 plus pks17) in Mycobacterium tuberculosis H37Rv generates a mutant incapable of producing monomethyl branched unsaturated C(16) to C(20) fatty acids that are minor constituents of acyltrehaloses and sulfolipids. The msl5 mutation did not cause any significant change in the acyl lipid composition and also did not affect growth in culture, in mouse alveolar macrophage cell line MH-S, or in the murine lung.
Dubey
Vinod S
VS
Biomolecular Science Center, University of Central Florida, Orlando, Florida 32816, USA.
Sirakova
Tatiana D
TD
Cynamon
Michael H
MH
Kolattukudy
Pappachan E
PE
D000214:Acyl Coenzyme A / Q000378:metabolism*; D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis* / Q000737:chemistry; D008168:Lung / Q000382:microbiology; D016676:Macrophages, Alveolar / Q000382:microbiology; D051379:Mice; D008810:Mice, Inbred C57BL; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D014397:Tuberculosis, Pulmonary / Q000382:microbiology*; D014774:Virulence
D000214:Acyl Coenzyme A; D001426:Bacterial Proteins; D005231:Fatty Acids, Unsaturated; C015357:methylmalonyl-coenzyme A; D000217:Acyltransferases; C044110:mycocerosic acid synthase