title: An improved HPLC method for quantitative determination of six triterpenes in Centella asiatica extracts and commercial products.
abstract: An improved HPLC qualitative and quantitative method of six triterpenes (asiaticoside, madecassoside, asiatic acid, madecassic acid, terminolic acid, and asiaticoside-B) in Centella asiatica (raw plant material and preparations) is described in this paper. After 50 minutes the six active triterpenes were separated and detected in the methanolic extract at a limit of 0.01 microg/ml. The method uses a Phenomenex Aqua 5mu C18 (200 A) column as the stationary phase, a gradient mobile phase of water (0.1% TFA), acetonitrile (0.1% TFA), and methyl tert-butyl ether (0.1% TFA), and UV detection at 206 nm. The correlation coefficients for the calibration curves and the recovery rates ranged from 0.995 to 0.999 and from 98.39% to 100.02%, respectively. The qualitative and quantitative results are discussed.
authors.affiliation: National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, MS 38677, USA.
authors.forename: B T
authors.initials: BT
authors.lastname: Schaneberg
authors.forename: J R
authors.initials: JR
authors.lastname: Mikell
authors.forename: E
authors.initials: E
authors.lastname: Bedir
authors.forename: I A
authors.initials: IA
authors.lastname: Khan
mesh_terms: D002138:Calibration; D002214:Capsules; D028041:Centella / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D019587:Dietary Supplements / Q000032:analysis; D010936:Plant Extracts / Q000032:analysis; D012015:Reference Standards; D012996:Solutions; D014315:Triterpenes / Q000032:analysis*
chemical_list: D002214:Capsules; D010936:Plant Extracts; D012996:Solutions; D014315:Triterpenes