title: Purcopia, a Ty1-copia truncated retroelement in the genome of Claviceps purpurea.
abstract: Truncated copy of reverse transcriptase of Ty1/copia retroelement (Purcopia) was found as part of the species-specific RAPD 257(540) marker of Claviceps purpurea. A region of 94 bp with 78.9% identity to an unannotated region of the genomic clone of the rice blast fungus Pyricularia grisea (accession no. AQ162050) was found at the 5' end of the pseudogene. Comparison with database sequences revealed that Purcopia is close to the plant retroelements represented by Tto1, Ta1-3 and Bare-1, whereas the other fungal elements of the Ty1/copia type grouped with Hopscotch elements. Restriction patterns obtained by hybridization of the labeled marker to HindIII digested genomic DNA of various C. purpurea isolates contained multiple bands. The banding was individual and did not yield any species- or population-specific fragments or patterns.
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, 142 20 Prague, Czechia.
authors.forename: S
authors.initials: S
authors.lastname: Pazoutová
authors.forename: R
authors.initials: R
authors.lastname: Kolínská
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002967:Claviceps / Q000235:genetics*; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics*; D016681:Genome, Fungal; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D018626:Retroelements / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: D004271:DNA, Fungal; D018626:Retroelements