Diabetes differentially modulated receptor- and non-receptor-mediated relaxation in rat renal artery.
In this study, we have investigated the vasodilator response to acetylcholine under diabetic conditions in isolated renal arteries of Wistar rats. The effect of nitric oxide synthase (NOS) inhibition on acetylcholine-induced vasodilator response was investigated. We have also examined the effects of two endothelium-dependent agonists which induce receptor-dependent and receptor-independent vasodilator responses. Acetylcholine (10(-10) to 10(-4)M) produced a cumulative concentration-response curve in the renal arteries of both control and diabetic rats. The EC(50) values and maximal responses to acetylcholine were reduced relative to diabetic conditions. The vasodilator response to sodium nitroprusside (SNP) (10(-10) to 10(-5)M) was also investigated. SNP produced a cumulative concentration-dependent vasodilator response, which was not affected under diabetic conditions. To confirm the nitric oxide component of acetylcholine-induced vasodilator response, L-nitro-methyl arginine ester (L-NAME) (10(-4)M) was added to the Krebs' solution. The maximal vasodilator response to acetylcholine was reduced in the presence of L-NAME (10(-4)M) in both control and diabetic renal preparations, with greater attenuation in the diabetic conditions. In order to examine the possible contribution of receptor dysfunction in diabetes, the vasodilator response to ADP (receptor-dependent agonist) and the calcium ionophore A23187 (receptor-independent agonist) were investigated. ADP (10(-10) to 10(-5)M) produced a concentration-dependent vasodilator response in preparations from both control and diabetic rats. The maximal vasodilator response to ADP was significantly reduced in the renal arteries from diabetic animals. However, A23187 (10(-10) to 10(-5)M); the receptor-independent agonist, produced a concentration-dependent vasodilator response in both control and diabetic rat preparations. There was no significant change in the EC(50) values or maximal vasodilator responses to A23187 under diabetic conditions. In conclusion, our results indicated that acetylcholine-induced vasodilatation in the isolated renal arteries of streptozotocin (STZ)-induced diabetic rats was attenuated under diabetic conditions. The reduction in acetylcholine-induced vasodilatation may be attributed to acetylcholine receptor dysfunction. This is based on the results from this study in which the vasodilator response to the receptor-independent agonist A23187 were maintained, while that of the receptor-dependent agonist ADP was attenuated under diabetic conditions.
Yousif
Mariam H M
MH
Department of Pharmacology & Toxicology, Faculty of Medicine, Kuwait University, P.O. Box 24923, Safat 13110, Kuwait. Mariam@hsc.kuniv.edu.kw
D000109:Acetylcholine / Q000494:pharmacology*; D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D000001:Calcimycin / Q000494:pharmacology*; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D045462:Endothelium-Dependent Relaxing Factors; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D009599:Nitroprusside / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012077:Renal Artery / Q000187:drug effects* / Q000503:physiopathology; D014664:Vasodilation / Q000187:drug effects*; D014665:Vasodilator Agents / Q000819:agonists*
D045462:Endothelium-Dependent Relaxing Factors; D004791:Enzyme Inhibitors; D014665:Vasodilator Agents; D009599:Nitroprusside; D000001:Calcimycin; D000244:Adenosine Diphosphate; D019001:Nitric Oxide Synthase; D000109:Acetylcholine; D019331:NG-Nitroarginine Methyl Ester