title: [Passage adaptability of candidate strains for hemorrhagic fever with renal syndrome purified vaccine in Vero cells and their immunogenicity].
abstract: OBJECTIVE
  To adapt the candidate strains of hemorrhagic fever with renal syndrome (HFRS) purified vaccine to Vero cells and to study their antigenicity and immunogenicity.
  
  
  METHODS
  The viral strains H8207 (Hantaan virus, HTN) and Y86013 (Seoul virus, SEO) were continuously propagated in Vero cell by the terminal dilution method and studied the characteristics of virus multiplication, viral titers and the amounts of virus antigen after serial passages. Three batches of crude monovalent inactivated vaccine were developed using the different passages of these 2 viral strains.
  
  
  RESULTS
  The strains H8207 and Y86013 adapted to Vero cells and stably grew on the cells with high titers. Rabbits immunized with the crude vaccines of H8207 and Y86013 showed 100% sero-conversion and the neutralizing antibody titers of the rabbit immune sera reached 1?10 at 4 weeks after 2 times of immunization.
  
  
  CONCLUSIONS
  The results suggest that these 2 candidate strains had adapted to Vero cells, possessed high titers and good immunogenicity and be feasible to prepare the HFRS purified vaccine in Vero cells.
authors.affiliation: Department of Etiology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Cai-fang
authors.initials: CF
authors.lastname: Xue
authors.forename: Jiang-qiu
authors.initials: JQ
authors.lastname: Liu
authors.forename: Zhong-yi
authors.initials: ZY
authors.lastname: Li
authors.forename: Yong-xing
authors.initials: YX
authors.lastname: Fan
authors.forename: Lu
authors.initials: L
authors.lastname: Xu
authors.forename: Hui
authors.initials: H
authors.lastname: Liao
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002522:Chlorocebus aethiops; D018063:Hantaan virus / Q000254:growth & development / Q000276:immunology*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000517:prevention & control; D051379:Mice; D009500:Neutralization Tests; D011817:Rabbits; D029263:Seoul virus / Q000254:growth & development / Q000276:immunology*; D014611:Vaccination; D015164:Vaccines, Inactivated / Q000276:immunology; D014709:Vero Cells; D014765:Viral Vaccines / Q000096:biosynthesis* / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D015164:Vaccines, Inactivated; D014765:Viral Vaccines