Identification and sequence analysis of Potato yellow vein virus capsid protein minor gene.
Potato yellow vein virus (PYVV) is a whitefly-transmitted (Trialeurodes vaporariorum) closterovirus (WTC) with an as yet unidentified genome composition. PYVV dsRNA preparations consist of three high molecular weight dsRNA species (dsRNAs 1, 2 and 3) 8.0, 5.5 and 4.0 kbp in size respectively, as well as two low molecular weight dsRNA species of 2.0 and 1.8 kbp (denoted x and y). The PYVV capsid protein minor (CPm) gene was identified on the dsRNA 3 species, and was subsequently cloned and sequenced. The PYVV CPm gene is 2022 nucleotides long and putatively encodes a protein with estimated size 77.5 kDa. The PYVV CPm gene product is considerably larger than the equivalent proteins encoded by the bipartite criniviruses, Lettuce infectious yellows virus (LIYV) and Cucurbit yellow stunting disorder virus (CYSDV) (52 and 53 kDa, respectively). The PYVV CPm possesses a centralized domain which is absent from both the LIYV and CYSDV CPm counterparts. Pairwise comparisons as well as phylogenetic analysis based on the available amino acid sequences of the CPm of various WTCs, showed that PYVV is closely related to LIYV, CYSDV and also Beet pseudo-yellows virus.
Livieratos
Ioannis C
IC
Department of Biological Sciences, Imperial College of Science, Technology and Medicine, Sir Alexander Fleming Building, Imperial College Road, London SW7 2AZ, UK.
Muller
Giovanna
G
Salazar
Luis F
LF
Eliasco
Eleonora
E
Coutts
Robert H A
RH
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D036022:Capsid Proteins / Q000235:genetics*; D029381:Closteroviridae / Q000235:genetics*; D019143:Evolution, Molecular; D006430:Hemiptera / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012330:RNA, Double-Stranded / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D011198:Solanum tuberosum / Q000821:virology
D036022:Capsid Proteins; D012330:RNA, Double-Stranded