Neither spinal gamma-aminobutyric acid-A nor strychnine-sensitive glycine receptor systems are the sole mediators of halothane depression of spinal dorsal horn sensory neurons.
Inhaled anesthetics depress the response of spinal dorsal horn low-threshold (LT) neurons to peripheral receptive field stimulation. Part of that depression may be mediated by anesthetic interactions with gamma-aminobutyric acid type A (GABA(A)) and strychnine-sensitive glycine inhibitory neurotransmitter systems. In this electrophysiological study, we attempted to antagonize halothane depression of LT neurons by administering bicuculline (a competitive GABA(A) antagonist) and/or strychnine (a competitive glycine antagonist) systemically, alone or in combination, to decerebrate, spinal cord-transected rats. We observed that both bicuculline and strychnine, alone or in combination, significantly but only partially reversed halothane depression of LT neuronal responses to receptive field stimulation. The inability of bicuculline and strychnine, alone or in combination, to completely reverse halothane depression suggests that although GABA(A) and glycine systems are involved in the observed halothane depression, additional mechanisms of action are also required for halothane depression of LT spinal sensory neurons.


CONCLUSIONS
The results of this study support the hypothesis that halothane depression of spinal sensory neurons is mediated, but not completely, by the anesthetic effects on gamma-aminobutyric acid type A and strychnine-sensitive glycine neurotransmitter systems.
Yamauchi
Masanori
M
Department of Anesthesiology, Yale University School of Medicine, New Haven, Connecticut.
Shimada
Steven G
SG
Sekiyama
Hiroshi
H
Collins
J G
JG
D000200:Action Potentials / Q000187:drug effects; D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D018756:GABA Antagonists / Q000494:pharmacology; D018684:Glycine Agents / Q000494:pharmacology*; D006221:Halothane / Q000494:pharmacology*; D008297:Male; D010812:Physical Stimulation; D020671:Posterior Horn Cells / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000187:drug effects / Q000502:physiology*; D018009:Receptors, Glycine / Q000187:drug effects / Q000502:physiology*; D013116:Spinal Cord / Q000601:surgery; D013331:Strychnine / Q000494:pharmacology*
D018685:Anesthetics, Inhalation; D018756:GABA Antagonists; D018684:Glycine Agents; D011963:Receptors, GABA-A; D018009:Receptors, Glycine; D013331:Strychnine; D006221:Halothane; D001640:Bicuculline