Tea can protect against aberrant crypt foci formation during azoxymethane induced rat colon carcinogenesis.
Tea shows many health promoting activities including chemopreventive action during carcinogenesis due to the presence of antioxidative polyphenolic constituents. The present experiment evaluated the anticarcinogenic role of black tea infusion on azoxymethane induced colonic preneoplastic lesions, the aberrant crypt foci in Sprague-Dawley rats. Rats were injected with azoxymethane (15 mg/kg b.w.) and received oral administration of 1% and 2% (w/v) tea infusions from first day of carcinogen application. This treatment was continued for twelve weeks and assessed for aberrant crypt foci and compared with untreated carcinogen control group. Levels of lipid peroxidation were determined in liver as well as in colon tissue. During initiation phase of carcinogenesis, glutathione-S-transferase (GST) and glutathione peroxidase (GPx) activities were also evaluated. Significant reduction in the number of aberrant crypt foci and levels of lipid peroxidation among the tea-treated groups were observed. Induction of GST and GPx activities was noted during the initiation phase of carcinogenesis. Results of the present study indicate that the protective effect of black tea infusions may be due to an outcome of antioxidative influence of tea components on azoxymethane induced carcinogenesis.
Sengupta
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Dept. of Cancer Chemoprevention, Chittaranjan National Cancer Institute, Kolkata, India. archana_sen@yahoo.com
Ghosh
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Das
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D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology; D001397:Azoxymethane; D001681:Biological Assay; D002273:Carcinogens; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000188:drug therapy*; D005419:Flavonoids / Q000494:pharmacology; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D015227:Lipid Peroxidation; D008297:Male; D009374:Neoplasms, Experimental; D010636:Phenols / Q000494:pharmacology; D059808:Polyphenols; D011230:Precancerous Conditions / Q000517:prevention & control*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013662:Tea; D013997:Time Factors
D000975:Antioxidants; D002273:Carcinogens; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D013662:Tea; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase; D001397:Azoxymethane