title: High expression of a human lactoferrin in transgenic tobacco cell cultures.
abstract: Transgenic Nicotiana tabacum cell lines were developed expressing the human lactoferrin gene driven by the oxidative stress-inducible peroxidase (SWPA2) promoter. Western blot analysis showed the accumulation of both the full-length human lactoferrin protein as well as a immuno-reactive truncated fragment. Accumulation of human lactoferrin as monitored by ELISA increased proportionally to cell growth and reached a maximal (up to 4.3% of total soluble proteins) at the stationary phase of growth. Protein extracts from transgenic tobacco cells exhibited antibacterial activity.
authors.affiliation: Laboratory of Plant Cell Biotechnology, Korea Research Institute of Bioscience and Biotechnology, Oun-Dong 52, Yusong, Daejeon 305-806, Korea.
authors.forename: Sun-Mee
authors.initials: SM
authors.lastname: Choi
authors.forename: Ok-Sun
authors.initials: OS
authors.lastname: Lee
authors.forename: Suk-Yoon
authors.initials: SY
authors.lastname: Kwon
authors.forename: Sang-Soo
authors.initials: SS
authors.lastname: Kwak
authors.forename: Dae-Yeul
authors.initials: DY
authors.lastname: Yu
authors.forename: Haeng-Soon
authors.initials: HS
authors.lastname: Lee
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002478:Cells, Cultured; D004926:Escherichia coli / Q000187:drug effects; D005786:Gene Expression Regulation; D006801:Humans; D007781:Lactoferrin / Q000096:biosynthesis* / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D011786:Quality Control; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D012486:Salmonella typhimurium / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D011994:Recombinant Proteins; D007781:Lactoferrin