Comparative mapping in the pig: localization of 214 expressed sequence tags.
In total, 214 ESTs (Expressed Sequence Tags) were assigned to the porcine gene map by using somatic cell hybrid mapping, radiation hybrid mapping, and FISH. The ESTs were isolated from a porcine small intestine cDNA library on the basis of significant sequence identity with human annotated genes. In total, 390 primer pairs were designed primarily in the 3' UTR of the sequences. Overall, 58.6% of the ESTs were successfully mapped by this approach. In total, 191 of the localizations are in agreement with the human comparative map, strongly indicating that these represent true orthologous genes. The remaining 23 ESTs provide new comparative mapping data, which should be considered as preliminary until confirmed by other studies. Our mapping efforts provide a significant contribution to the porcine map as well as to the comparative map for human and pig.
Cirera
Susanna
S
Department of Animal Science and Animal Health, Division of Genetics, The Royal Veterinary and Agricultural University, Groennegaardsvej 3, 1870 Frederiksberg C, Denmark.
Jørgensen
Claus B
CB
Sawera
Milena
M
Raudsepp
Terje
T
Chowdhary
Bhanu P
BP
Fredholm
Merete
M
D000818:Animals; D002874:Chromosome Mapping; D017931:DNA Primers; D020224:Expressed Sequence Tags; D017404:In Situ Hybridization, Fluorescence; D021101:Radiation Hybrid Mapping; D034421:Sus scrofa / Q000235:genetics*
D017931:DNA Primers