title: Antibody repertoire and gene expression profile: implications for different developmental and functional traits of splenic and peritoneal B-1 lymphocytes.
abstract: In L2 mice, a high expression level of the transgenic lambda2(315) L chain results in nearly complete exclusion of endogenous L chains and a predominance of B-1a cells. In this study, we show that splenic and peritoneal B-1a cells differ considerably in their Ab repertoire and gene expression profile. Splenic B-1a cells exhibit a more diversified repertoire under L chain limitation. Despite oligoclonal overlaps between both B-1a compartments, some B cell receptor specificities are clearly restricted to the peritoneum. The capacity of peritoneal B-1a cells to enter the splenic B-1a compartment was found to be very limited. Gene expression profiling revealed genes up-regulated in splenic B-1a cells that are involved in mediating specialized first-line-of-defense effector functions and interaction with T cells. Thus, splenic and peritoneal B-1a cells differ not only in their developmental program but also in functional properties.
authors.affiliation: Molecular Immunology, German Research Center for Biotechnology, Braunschweig, Germany. kkr@gbf.de
authors.forename: Karsten
authors.initials: K
authors.lastname: Kretschmer
authors.forename: Anke
authors.initials: A
authors.lastname: Jungebloud
authors.forename: Jana
authors.initials: J
authors.lastname: Stopkowicz
authors.forename: Britta
authors.initials: B
authors.lastname: Stoermann
authors.forename: Reinhard
authors.initials: R
authors.lastname: Hoffmann
authors.forename: Siegfried
authors.initials: S
authors.lastname: Weiss
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000916:Antibody Diversity / Q000235:genetics; D000918:Antibody Specificity / Q000235:genetics; D015703:Antigens, CD / Q000096:biosynthesis; D001202:Ascitic Fluid / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D016175:B-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D018122:B7-1 Antigen / Q000096:biosynthesis; D051940:B7-2 Antigen; D001483:Base Sequence; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D002465:Cell Movement / Q000235:genetics / Q000276:immunology; D002999:Clone Cells; D018985:Epitopes, B-Lymphocyte / Q000276:immunology; D020869:Gene Expression Profiling / Q000379:methods; D007143:Immunoglobulin Heavy Chains / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics*; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000276:immunology; D010713:Phosphatidylcholines / Q000276:immunology; D013045:Species Specificity; D013154:Spleen / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D015854:Up-Regulation / Q000235:genetics / Q000276:immunology
chemical_list: D015703:Antigens, CD; D018122:B7-1 Antigen; D051940:B7-2 Antigen; C497965:Cd86 protein, mouse; D018985:Epitopes, B-Lymphocyte; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D008562:Membrane Glycoproteins; D010713:Phosphatidylcholines