The gammaherpesvirus chemokine binding protein binds to the N terminus of CXCL8.
Viruses encode proteins that disrupt chemokine responses. The murine gammaherpesvirus 68 gene M3 encodes a chemokine binding protein (vCKBP-3) which has no sequence similarity to chemokine receptors but inhibits chemokine receptor binding and activity. We have used a panel of CXCL8 analogs to identify the structural requirements for CXCL8 to bind to vCKBP-3 in a scintillation proximity assay. Our data suggest that vCKBP-3 acts by mimicking the binding of chemokine receptors to CXCL8.
Webb
Louise M C
LM
Department of Medicine, Division of Virology, University of Cambridge, Cambridge, United Kingdom.
Clark-Lewis
Ian
I
Alcami
Antonio
A
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D019743:Chemokines, CXC / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D018142:Gammaherpesvirinae / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D011485:Protein Binding; D014764:Viral Proteins / Q000378:metabolism*
D019743:Chemokines, CXC; C118862:M3 protein, Murine gammaherpesvirus; D014764:Viral Proteins