Continuous isomerization of D-fructose to D-mannose by immobilized Agrobacterium radiobacter cells.
D-Fructose was isomerized to D-mannose using immobilized Agrobacterium radiobacter that produces a thermostable mannose isomerase. The cells were immobilized by adsorption on chitosan or by glutaraldehyde crosslinking in the presence of albumin. Optimum conditions for mannose isomerase activity were 60 degrees C and pH 7.5. Continuous reaction at 55 degrees C was achieved with immobilized cells packed in a column to produce D-mannose.
Hirose
Jun
J
Department of Applied Chemistry, Faculty of Engineering, Miyazaki University, Miyazaki 889-2192, Japan. jhirose@cc.miyazaki-u.ac.jp
Kinoshita
Yuka
Y
Fukuyama
Seijiro
S
Hayashi
Sachio
S
Yokoi
Haruhiko
H
Takasaki
Yoshiyuki
Y
D000418:Albumins; D019747:Aldose-Ketose Isomerases / Q000378:metabolism*; D018914:Cells, Immobilized / Q000201:enzymology / Q000378:metabolism; D002686:Chitin / Q000031:analogs & derivatives*; D048271:Chitosan; D004789:Enzyme Activation; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism; D005632:Fructose / Q000378:metabolism*; D005976:Glutaral; D007536:Isomerism; D008358:Mannose / Q000096:biosynthesis*; D011786:Quality Control; D012231:Rhizobium / Q000145:classification / Q000378:metabolism*; D013696:Temperature
D000418:Albumins; D004800:Enzymes, Immobilized; D002686:Chitin; D005632:Fructose; D048271:Chitosan; D019747:Aldose-Ketose Isomerases; C032728:mannose isomerase; D008358:Mannose; D005976:Glutaral