title: Development of ultra-micro flow analysis with chemiluminescence detector.
abstract: We proposed to combine an ultra-micro flow analysis instrument using the fused-silica capillary and the CL detector. The CL reaction of luminol and hydrogen peroxide was adopted and the batch-type CL detection cell was used. Luminol and isoluminol-labeled protein as a model were sensitively and reproducibly detected with very small amounts of quantitative reagents. The analyses were repeated at least 100 times without any treatments such as washing capillaries or exchanging the hydrogen peroxide solution. The present system successfully promoted the miniaturization, simplification, and sensitization of the analytical system.
authors.affiliation: Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University, Kyotanabe, Kyoto 610-0321, Japan. ktsukago@mail.doshisha.ac.jp
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Tsukagoshi
authors.forename: Keiko
authors.initials: K
authors.lastname: Taga
authors.forename: Riichiro
authors.initials: R
authors.lastname: Nakajima