title: Correlation of helical and incremental high-resolution thin-section computed tomographic imaging with histomorphometric quantitative evaluation of lungs in dogs.
abstract: OBJECTIVE
  To develop protocols for helical computed tomography (CT) and axial high-resolution CT (HRCT) of lungs and correlate densitometric CT values with morphometric and histologic data for normal pulmonary tissue in dogs.
  
  
  METHODS
  8 healthy adult dogs.
  
  
  METHODS
  2 dogs were used to establish a protocol for helical CT and HRCT of lungs. Six dogs were used to acquire densitometric CT data regarding normal lungs. After the dogs were euthanatized, their lungs were fixed and sampled for morphometric and histologic evaluation. Four CT acquisitions were compared by means of paired t tests.
  
  
  RESULTS
  For normal lung tissue of dogs, mean densitometric CT value obtained during helical CT scans reconstructed in a sharp algorithm was -846 Hounsfield units. Values obtained via helical CT or HRCT acquisitions and reconstructed with sharp or standard algorithms did not differ significantly. Morphometric analysis was used to determine the proportion of lung parenchymal (82%) and non-parenchymal tissue (18%). Alveolar size, estimated by mean linear intercept, was approximately 172 microm, and alveolar surface area-to-volume ratio was 0.024 to 0.026 microm(-1). Histologic evaluation confirmed the presence of normal lung tissue.
  
  
  CONCLUSIONS
  Correlation of densitometric CT data with morphometric and histologic findings and the establishment of helical CT and HRCT protocols were attained; clinical use of this information may facilitate investigation of pulmonary disease in dogs. Sharp helical CT acquisitions were preferred because of better lung parenchyma detail and rapid image acquisitions, compared with HRCT.
authors.affiliation: Department of Veterinary Clinical Sciences, The Ohio State University, Columbus 43210, USA.
authors.forename: Federica
authors.initials: F
authors.lastname: Morandi
authors.forename: John S
authors.initials: JS
authors.lastname: Mattoon
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Lakritz
authors.forename: James R
authors.initials: JR
authors.lastname: Turk
authors.forename: Erik R
authors.initials: ER
authors.lastname: Wisner
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000175:diagnosis; D004285:Dogs; D005260:Female; D008168:Lung / Q000033:anatomy & histology*; D008171:Lung Diseases / Q000175:diagnosis / Q000662:veterinary; D008297:Male; D016707:Tissue Fixation; D014057:Tomography, X-Ray Computed / Q000379:methods* / Q000662:veterinary*