title: Purification and crystallization of a LysR-type transcriptional regulator CBNR from Ralstonia eutropha NH9.
abstract: CbnR, a LysR-type transcriptional regulator from Ralstonia eutropha NH9, has been crystallized by the vapor-diffusion method. It is intriguing to note that the different mixing ratios between the protein and reservoir solutions resulted in the different crystal forms. These crystals have the symmetry of the orthorhombic system with space groups P2(1)1(i)2 and P2(1)2(1)2(1).
authors.affiliation: Department of BioEngineering, Nagaoka University of Technology, Niigata, Japan.
authors.forename: Shin
authors.initials: S
authors.lastname: Muraoka
authors.forename: Rumi
authors.initials: R
authors.lastname: Okumura
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Uragami
authors.forename: Takamasa
authors.initials: T
authors.lastname: Nonaka
authors.forename: Naoto
authors.initials: N
authors.lastname: Ogawa
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Miyashita
authors.forename: Toshiya
authors.initials: T
authors.lastname: Senda
mesh_terms: D016960:Agrobacterium tumefaciens / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D001665:Binding Sites; D018360:Crystallography, X-Ray; D020118:Cupriavidus necator / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation; D014157:Transcription Factors / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D014157:Transcription Factors; C073470:LysR protein, Bacteria; D004247:DNA