title: Measurement of retinoids and beta-carotene 15,15'-dioxygenase activity in HR-1 hairless mouse skin with UV exposure.
abstract: We investigated the vitamin A status and beta-carotene 15,15'-dioxygenase activity in hairless mice with UV exposure to assess the regulation of vitamin A metabolism after UV irradiation. HR-1 hairless mice were irradiated with UV at 3 J/cm2 for 5 d. After UV irradiation, the mice were sacrificed and samples were obtained to analyze the retinoid concentration, expression of RXR-alpha, and beta-carotene 15,15'-dioxygenase activity. After UV exposure, the skin retinoid concentration was significantly lower as well as the expression of RXR-alpha. Higher skin beta-carotene dioxygenase activity was observed in the UV group as compared to the control group. We found no significant differences in the alpha-tocopherol concentration or acrolein levels in the skins of the two groups. In conclusion, the elevation of beta-carotene 15,15'-dioxygenase activity in hairless mice after UV exposure may be a response to reduction of the skin retinoid concentration.
authors.affiliation: Department of Pediatrics, Osaka Medical College, 2-7 Daigakumachi, Takatsuki, Osaka 569-8686, Japan.
authors.forename: Atsuko
authors.initials: A
authors.lastname: Takeda
authors.forename: Takao
authors.initials: T
authors.lastname: Morinobu
authors.forename: Kimitaka
authors.initials: K
authors.lastname: Takitani
authors.forename: Mieko
authors.initials: M
authors.lastname: Kimura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tamai
mesh_terms: D000818:Animals; D015153:Blotting, Western; D008099:Liver / Q000737:chemistry; D008297:Male; D051379:Mice; D008812:Mice, Hairless; D010105:Oxygenases / Q000378:metabolism*; D018168:Receptors, Retinoic Acid / Q000032:analysis; D047488:Retinoid X Receptors; D012176:Retinoids / Q000032:analysis* / Q000097:blood; D012867:Skin / Q000737:chemistry / Q000201:enzymology* / Q000528:radiation effects*; D014157:Transcription Factors / Q000032:analysis; D014466:Ultraviolet Rays; D024502:alpha-Tocopherol / Q000032:analysis; D050559:beta-Carotene 15,15'-Monooxygenase
chemical_list: D018168:Receptors, Retinoic Acid; D047488:Retinoid X Receptors; D012176:Retinoids; D014157:Transcription Factors; D010105:Oxygenases; C493359:Bco1 protein, mouse; D050559:beta-Carotene 15,15'-Monooxygenase; D024502:alpha-Tocopherol