Shotgun proteomics: integrating technologies to answer biological questions.
Proteomics is providing us with a variety of exciting new strategies to address biological questions. These strategies must integrate a series of seperative and analytical technologies to deal with the immense complexity involved. At some point in the process, the proteins are usually digested with a proteolytic enzyme to generate shorter peptides that are more easily analyzed by mass spectrometry. Shotgun proteomics relies on separation after this digestion step and takes advantage of tandem mass spectrometry to infer the amino acid sequence of individual peptides. Advances in quantitation, and the ability to find sites of post-translational modification are expanding the scope of questions that can be asked. The ultimate success of any proteomic experiment is dictated not only by an appropriate choice of seperative and analytical techniques, but also by making certain that the biological aspects of the experiment are focused and well designed.
McDonald
W Hayes
WH
Department of Cell Biology, SR-11, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92307, USA.
Yates
John R
JR
D000818:Animals; D064127:Aurora Kinases; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D010961:Plasmodium / Q000737:chemistry / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D015003:Yeasts / Q000737:chemistry / Q000378:metabolism
D010455:Peptides; D011506:Proteins; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases