Changes of balance between proteinase and their inhibitors in blood of pigs with high-velocity missile wounds.
OBJECTIVE
To study the effect of imbalance between lysosomal enzymes and their inhibitors in blood on disturbance of the local and whole body after trauma.


METHODS
The dynamic changes of lysosomal enzymes and proteinase inhibitors were studied in 12 pigs with femoral comminuted fractures in both hind limbs caused by high velocity missiles. Four normal pigs served as controls.


RESULTS
After injury, the activity of Cathepsin D in arterial plasma increased gradually and reached the highest level at 8 hours, acid phosphatase in serum began to increase at 12 hours and the value of serum elastase did not change significantly. The level of alpha1-antitrypsin, a proteinase inhibitor in plasma, decreased significantly in the early stage after injury [73.5%+/-6.4% and 81.0%+/-5.1% of the baseline value (1.67 micromol x ml(-1) x min(-1)+/- 0.29 micromol x ml(-1) x min(-1)) at l and 2 hours after injury, respectively, P<0.05], then increased gradually and was higher than the baseline value at 12 hours after injury.


CONCLUSIONS
Imbalance between lysosomal enzymes and proteinase inhibitors occurs soon after injury, which might result in continuous tissue damage and play an important role in the disturbance of general reaction after injury.
Zhou
Yuan-guo
YG
Molecular Biology Center, Research Institute of Surgery Daping Hospital, Third Military Medical University, Chongqing 400042, China. ygzhou@cta.cq.cn
Zhu
Pei-fang
PF
Zhou
Ji-hong
JH
Li
Xiao-yan
XY
D000135:Acid Phosphatase / Q000097:blood; D000818:Animals; D002402:Cathepsin D / Q000097:blood; D010450:Endopeptidases / Q000097:blood*; D005260:Female; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D010196:Pancreatic Elastase / Q000097:blood; D013552:Swine; D014948:Wounds, Gunshot / Q000097:blood*; D000515:alpha 1-Antitrypsin / Q000032:analysis*
D000515:alpha 1-Antitrypsin; D000135:Acid Phosphatase; D010450:Endopeptidases; D010196:Pancreatic Elastase; D002402:Cathepsin D