Contribution of ionotropic glutamate receptors to acute amphetamine-stimulated preproenkephalin mRNA expression in the rat striatum in vivo.
The present study evaluated the possible role of ionotropic glutamate receptors in mediating the inducible preproenkephalin (PPE) mRNA expression in the rat striatum in response to systemic administration of a psychostimulant amphetamine. A single injection of amphetamine (5 mg/kg, i.p.) increased PPE mRNA levels in the dorsal striatum as revealed via quantitative in situ hybridization. Pretreatment with the NMDA receptor antagonist, MK801 (0.5 mg/kg, i.p.) or 3-((R)-2-carboxypiperazin-4-yl)-propyl-1-phosphonic acid (10 mg/kg, i.p.), attenuated the amphetamine-induced PPE mRNA expression. A similar attenuation of the amphetamine-induced PPE expression, although to a lesser extent, was also observed following pretreatment with a kainate/AMPA receptor antagonist, 6,7-dinitroquinoxaline-2,3-dione (100-200 mg/kg, i.p.). Three antagonists alone had no significant effect on basal levels of PPE mRNAs in the dorsal striatum. These data indicate that both NMDA and kainate/AMPA receptors are involved in coupling extracellular signals evoked by acute stimulant exposure to PPE mRNA expression in striatal neurons.
Mao
Limin
L
Division of Pharmacology, School of Pharmacy, University of Missouri-Kansas City, 2411 Holmes Street, Kansas City, MO 64108, USA.
Wang
John Q
JQ
D000661:Amphetamine / Q000494:pharmacology*; D000818:Animals; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism; D004745:Enkephalins / Q000096:biosynthesis* / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D008297:Male; D011498:Protein Precursors / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D018092:Kainic Acid Receptors / Q000037:antagonists & inhibitors / Q000378:metabolism*
D000661:Amphetamine; D004745:Enkephalins; D011498:Protein Precursors; D012333:RNA, Messenger; D018092:Kainic Acid Receptors; C033660:preproenkephalin