Supplementation of vitamin E and selenium prevents hyperoxaluria in experimental urolithic rats.
Renal injury is considered as one of the prerequisites for calcium oxalate retention. In order to determine the role of lipid peroxidation related effects for hyperoxaluria, we evaluated the alterations in lipid peroxidation, antioxidants and oxalate synthesizing enzymes in lithogenic rats with response to vitamin E + selenium treatment. In kidney of lithogenic rats, the level of lipid peroxidation and the activities of oxalate synthesizing enzymes were found to be increased whereas the levels/activities of non-enzymatic and enzymatic antioxidants were found to be decreased. The urinary excretion of both oxalate and calcium were significantly elevated. Supplementation of lithogenic rats with vitamin E + selenium decreased the levels of lipid peroxides and the activities of oxalate synthesizing enzymes like glycolic acid oxidase (GAO), lactate dehydrogenase (LDH), xanthine oxidase (XO) with a concomitant increase in the activities of enzymatic antioxidants like superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glucose-6-phosphate dehydrogenase (G6PDH) and increased levels of non-enzymatic antioxidants like ascorbic acid, alpha-tocopherol and reduced glutathione (GSH). The urinary excretion of oxalate and calcium were normalized. The antioxidants vitamin E + selenium thereby protected from hyperoxaluria.
Santhosh Kumar
Mani
M
Department of Medical Biochemistry, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai 600 113, India. manis@musc.edu
Selvam
Ramasamy
R
D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001205:Ascorbic Acid / Q000032:analysis; D002129:Calcium Oxalate / Q000032:analysis; D002374:Catalase / Q000378:metabolism; D019587:Dietary Supplements; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005978:Glutathione / Q000032:analysis; D005979:Glutathione Peroxidase / Q000378:metabolism; D006959:Hyperoxaluria / Q000517:prevention & control*; D007668:Kidney / Q000737:chemistry; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation; D008099:Liver / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000008:administration & dosage*; D013482:Superoxide Dismutase / Q000378:metabolism; D014545:Urinary Calculi / Q000188:drug therapy* / Q000378:metabolism / Q000652:urine; D014810:Vitamin E / Q000008:administration & dosage* / Q000032:analysis; D014969:Xanthine Oxidase / Q000378:metabolism
D000975:Antioxidants; D014810:Vitamin E; D002129:Calcium Oxalate; D000429:Alcohol Oxidoreductases; D007770:L-Lactate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; C020821:glycollate oxidase; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase; D005978:Glutathione; D012643:Selenium; D001205:Ascorbic Acid