An optimized reverse transcription-polymerase chain reaction procedure for the amplification of low-copy Cyp2a3 mRNA in rat esophagus.
Robottom Ferreira
Anna B
AB
Departamento de Bioquímica, Instituto de Biologia Roberto Alcântara Gomes, Universidade do Estado do Rio de Janeiro, Pavilhão Américo Piquet Carneiro, Rio de Janeiro, RJ, Brazil.
Ribeiro Pinto
Luís F
LF
Albano
Rodolpho M
RM
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000096:biosynthesis / Q000235:genetics*; D065637:Cytochrome P-450 CYP2A6; D004947:Esophagus / Q000201:enzymology*; D008297:Male; D006899:Mixed Function Oxygenases / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis* / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D014199:Trehalose / Q000737:chemistry
D012333:RNA, Messenger; D014199:Trehalose; D006899:Mixed Function Oxygenases; C585519:Cyp2a3 protein, rat; D001189:Aryl Hydrocarbon Hydroxylases; D065637:Cytochrome P-450 CYP2A6