title: Crystallization and preliminary structure determination of the C-terminal truncated domain of the S-layer protein SbsC.
abstract: The C-terminal truncated form of the S-layer protein SbsC from Geobacillus stearothermophilus, rSbsC(31-844), has been crystallized by the vapour-diffusion method using polyethylene glycol 6000 as a precipitating agent. The crystals diffract to 3 A resolution using synchrotron radiation and belong to space group P2(1), with unit-cell parameters a = 57.24, b = 98.91, c = 108.62 A, beta = 94.34 degrees. One molecule is present in the asymmetric unit, which corresponds to a solvent content of 65%. Native and heavy-atom derivative data have been collected. The Pt derivative yielded two high-occupancy sites per molecule.
authors.affiliation: Institute of Chemistry/Structural Biology, Karl-Franzens-University Graz, Heinrichstrasse 28, 8010 Graz, Austria.
authors.forename: Tea
authors.initials: T
authors.lastname: Pavkov
authors.forename: Monika
authors.initials: M
authors.lastname: Oberer
authors.forename: Eva M
authors.initials: EM
authors.lastname: Egelseer
authors.forename: Margit
authors.initials: M
authors.lastname: Sára
authors.forename: Uwe B
authors.initials: UB
authors.lastname: Sleytr
authors.forename: Walter
authors.initials: W
authors.lastname: Keller
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D001665:Binding Sites; D003460:Crystallization; D018360:Crystallography, X-Ray; D001411:Geobacillus stearothermophilus / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000737:chemistry*; D017020:Methanosarcina / Q000378:metabolism; D010984:Platinum / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D013696:Temperature; D014961:X-Ray Diffraction
chemical_list: D001426:Bacterial Proteins; D008562:Membrane Glycoproteins; C055764:S-layer proteins; D011092:Polyethylene Glycols; D010984:Platinum