Roles of interleukin-10 in acute graft-versus-host disease and graft rejection.
OBJECTIVE
To study the role of interleukin (IL)-10 in acute-graft-versus-host disease (aGVHD) and graft rejection.


METHODS
Serum concentrations of IL-10 in 28 patients who underwent allogeneic hematopoietic stem cell transplantation (allo-HSCT) were measured by enzymed-linked immunosorbent assay (ELISA). IL-10 gene expression in peripheral mononuclear cells was measured by reverse-transcriptase polymerase chain reaction (RT-PCR) after transplantation.


RESULTS
Seven patients developed grade I GVHD, 7 patients developed grade II-IV GVHD, 4 patients had graft rejection. Before transplantation, the concentrations of IL-10 were higher in patients who later did not developed aGVHD. After transplantation, IL-10 levels increased in patients without aGVHD, but decreased in patients with aGVHD or graft rejection. And IL-10mRNA was more frequent in patients without aGVHD compared to those with aGVHD.


CONCLUSIONS
IL-10 plays a negative role in the development of aGVHD and graft rejection.
Ju
Xiaoping
X
Department of Hematology, Changhai Hospital, Shanghai 200433, China. xubin@smmu.edu.cn
Wang
Jianmin
J
Xu
Bin
B
Cao
Yongbin
Y
Lü
Shuqing
S
D000208:Acute Disease; D000328:Adult; D002648:Child; D006084:Graft Rejection / Q000209:etiology*; D006086:Graft vs Host Disease / Q000209:etiology*; D006801:Humans; D016753:Interleukin-10 / Q000097:blood / Q000235:genetics / Q000502:physiology*; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis
D012333:RNA, Messenger; D016753:Interleukin-10