title: Estrogen therapy induces collateral and microvascular remodeling.
abstract: Estrogen increases proliferation and migration of cultured endothelial cells and perfusion of ischemic hindlimbs of rabbits. We tested the hypothesis that estrogen is angiogenic and arteriogenic in the heart during progressive coronary occlusion. Ovariectomized (OVX) and 17beta-estradiol (1 mg.kg(-1).wk(-1) im)-treated OVX (OVX-ES) female New Zealand White rabbits were instrumented with an ameroid occluder on a proximal coronary artery. Four weeks after implantation of an ameroid occluder, we measured myocardial perfusion with microspheres at rest and during adenosine-induced maximal vasodilation. The heart was fixed by perfusion at physiological pressure, and capillary angiogenesis and remodeling were assessed by image analysis of tissue sections in collateral-dependent myocardium. Coronary conductance was higher at rest and during maximal vasodilation in collateral-dependent myocardium of OVX-ES than OVX rabbits. Estrogen treatment increased the wall-to-lumen ratio of collateral vessels while it decreased the wall-to-lumen ratio of noncollateral arteries in normal regions. In normal and collateral-dependent myocardium, mean capillary diameter and capillary volume density were greater in OVX-ES rabbits. However, estrogen had no effect on capillary length density in either region of the myocardium. These data suggest that estrogen induces remodeling of the collateral vasculature and may stimulate growth of the resistance vessels, thereby providing protection during development of a gradual coronary occlusion.
authors.affiliation: Medical Services (111), VA Medical Center, 601 Highway 6 West, Iowa City, IA 52246, USA. kathryn-lamping@uiowa.edu
authors.forename: Kathryn G
authors.initials: KG
authors.lastname: Lamping
authors.forename: Lance P
authors.initials: LP
authors.lastname: Christensen
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Tomanek
mesh_terms: D000818:Animals; D001794:Blood Pressure; D002196:Capillaries / Q000187:drug effects / Q000648:ultrastructure; D003097:Collateral Circulation / Q000187:drug effects*; D003326:Coronary Circulation / Q000187:drug effects; D003327:Coronary Disease / Q000188:drug therapy*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D008854:Microscopy, Electron; D017202:Myocardial Ischemia / Q000188:drug therapy*; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D010052:Ovariectomy; D011817:Rabbits
chemical_list: D004958:Estradiol