[Changes in the percentage of human leukocyte antigen DR in CD(+)(14) monocytes in burned patients].
OBJECTIVE
To observe the changes in the percentage of CD(+)(14) monocytes expressing human leukocyte antigen DR(HLA-DR) in burn patients.


METHODS
Thirty burn patients were divided into three groups according to body surface area burned: severe burn group [>60% total body surface area(TBSA)]; moderate burn group (25%-30% TBSA), mild burn group(<10% TBSA). Blood samples were collected on 3, 7, 14, 28 and 49 postburn days(PBD), and CD(+)(14) monocyte HLA-DR expression was determined with monoclonal antibody staining and flow cytometry.


RESULTS
After burn injury, the percentage of monocytes expressing HLA-DR in mild burned patients was (58.80+/-5.60)%. The monocyte HLA-DR level was significantly reduced to below 30% on 3 PBD in both severely burned patients and moderately burned patients[(4.30+/-1.50)% and (13.86+/-2.40)%] respectively. The level of monocyte HLA-DR in moderately burned patients recovered on 21 PBD to above 30% while that of severely burned patients continued to be lowered till discharge from the hospital. There were statistical significant differences in HLA-DR expression between severely burned patients and moderately burned patients on 3, 7 and 14 PBD.


CONCLUSIONS
The results suggested that immunity paralysis lasted for a long time in severely burned patients, and the percentage of monocytes expressing HLA-DR could be an important index in evaluating immune function of burn patients. Therefore, therapy to enhance immunity may be beneficial in the treatment of burn patients.
Liu
Jing-jie
JJ
Burn Institute, 304th Hospital of People's Liberation Army, Beijing 100037, China.
Hu
Sen
S
Dong
Ning
N
Sun
Dan
D
Chai
Jia-ke
JK
Zhu
Jing-min
JM
Sheng
Zhi-yong
ZY
D000293:Adolescent; D000328:Adult; D002056:Burns / Q000097:blood* / Q000276:immunology*; D005260:Female; D006684:HLA-DR Antigens / Q000097:blood / Q000276:immunology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism; D018746:Systemic Inflammatory Response Syndrome; D055815:Young Adult
D006684:HLA-DR Antigens