A metabolic mechanism for the detrimental effect of exogenous glucose during cardiac storage.
The purpose of this study was to clarify the metabolic events that explain why supplemental glucose is detrimental during cardiac storage. Four solutions were used to flush and store porcine hearts: St. Thomas Hospital Solution (STHS), University of Wisconsin (UW) solution, and UW + 90 mM histidine, and UW + 90 mM histidine + 11 mM glucose. Despite equivalent increases in lactate in the two histidine-buffered groups throughout 10 h of storage, glycogen utilization was evident in the group without supplemental glucose. The presence of glucose resulted in a reduction in energy production, presumably mediated by direct inhibition of glycogenolysis. Furthermore, UW + histidine was the only group to show consistent improvements in ATP and total adenylates. It was concluded that inclusion of the buffering agent, histidine, to UW solution promotes anaerobic energy production as a result, in part, of preserved high levels of the regulatory control enzyme, phosphofructokinase.
Pulis
Randy P
RP
Surgical Medical Research Institute, University of Alberta, Edmonton, Alberta, Canada.
Wu
Beatrice M
BM
Zhu
Jay Z
JZ
Castillo
Erika G
EG
Kneteman
Norman M
NM
Churchill
Thomas A
TA
D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D001834:Body Water; D004734:Energy Metabolism; D005947:Glucose / Q000008:administration & dosage* / Q000378:metabolism; D024981:Glycogen Phosphorylase / Q000378:metabolism; D006019:Glycolysis; D006639:Histidine / Q000008:administration & dosage; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D009206:Myocardium / Q000201:enzymology / Q000378:metabolism*; D009926:Organ Preservation; D019188:Organ Preservation Solutions; D010725:Phosphocreatine / Q000378:metabolism; D010732:Phosphofructokinase-1 / Q000378:metabolism; D013552:Swine
D000227:Adenine Nucleotides; D007773:Lactates; D019188:Organ Preservation Solutions; D010725:Phosphocreatine; D006639:Histidine; D007521:Isocitrate Dehydrogenase; D024981:Glycogen Phosphorylase; D010732:Phosphofructokinase-1; D005947:Glucose