[Protection on proliferation of intestinal epithelial cells against hypoxia-reoxygenation through recombinant heat shock protein 70 adenovirus transfection].
OBJECTIVE
To investigate the protecting effect on proliferation of intestinal epithelial cells against hypoxia-reoxygenation through injury by transfection of recombinant adenovirus with whole long human heat shock protein 70 (HSP70) gene.


METHODS
The recombinant adenoviruses were constructed with whole long human HSP70 gene. Cultured intestinal epithelial cells (IEC-6 cells) were divided into four groups: control group without transfection and 24 hours, 48 hours, 72 hours after transfection groups. The expression of human HSP70 gene in transfected cells were detected by reverse transcription-polymerase chain reaction (RT-PCR) after 48 hours transfection. Cells in different groups after 1 hour were treated with hypoxia followed by 1 hour of reoxygenation, then cell viability, growth curve and proliferative index were evaluated in vitro.


RESULTS
The expression of human HSP70 gene in transfected IEC-6 cells was positive, while it was negative in control group. After treatment with hypoxia-reoxygenation, the cell viability in transfected groups was significantly higher than that of control (P<0.01), the proliferative index was also improved (P<0.05).


CONCLUSIONS
The proliferation ability of intestinal epithelial cells (IEC-6 cells) could be promoted by HSP70 gene transfection, which therefore could protect the IEC-6 cells against hypoxia-reoxygenation injury.
Li
Xiao-lu
XL
Institute of Burn Research, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China.
Peng
Yi-zhi
YZ
Yuan
Zhi-qiang
ZQ
Huang
Yue-sheng
YS
Yang
Zong-cheng
ZC
D000256:Adenoviridae / Q000235:genetics; D015687:Cell Hypoxia; D002460:Cell Line; D002470:Cell Survival; D004847:Epithelial Cells / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D007422:Intestines / Q000166:cytology; D010100:Oxygen / Q000378:metabolism; D014162:Transfection
D018840:HSP70 Heat-Shock Proteins; D010100:Oxygen