title: Monoclonal IgA antibody-mediated expulsion of Trichinella from the intestine of mice.
abstract: To assess the potential role of IgA antibody in expulsion of the nematode of the genus Trichinella from the intestine, a panel of IgA monoclonal antibodies (mAbs) were produced from the mesenteric lymph node cells from BALB/c mice orally vaccinated with irradiated muscle larvae of Trichinella britovi. One IgA mAb, HUSM-Tb1, formed immunoprecipitates on the surface of live muscle larvae, and by immunohistochemistry reacted with their stichocytes and cuticular surface, but not with those tissues of the adult stage or newborn larvae. Intraperitoneal injection of BALB/c mice with this mAb 5 h before challenge conferred a high level of protection (more than 95%) against T. britovi infection, when 2.0 mg of specific IgA/20 g body weight was given to a mouse. The same treatment produced a similar effect in SCID mice lacking functional T- and B-cells, indicating no requirement of synergistc T-cell factors for the effect. Passive transfer of the mAb at the time of challenge or later showed less or no effect upon worm expulsion. It is concluded that the mucosal IgA response, when adequately induced, can impede the establishment of infective Trichinella parasites in the mouse intestine.
authors.affiliation: Department of Parasitology, Hirosaki University School of Medicine, Hirosaki 036-8562, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Inaba
authors.forename: H
authors.initials: H
authors.lastname: Sato
authors.forename: H
authors.initials: H
authors.lastname: Kamiya
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000276:immunology*; D015153:Blotting, Western; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005849:Gerbillinae; D006825:Hybridomas; D018928:Immunity, Mucosal; D007116:Immunization, Passive; D007071:Immunoglobulin A, Secretory / Q000008:administration & dosage / Q000276:immunology*; D007150:Immunohistochemistry; D007411:Intestinal Diseases, Parasitic / Q000276:immunology* / Q000517:prevention & control; D007422:Intestines / Q000276:immunology / Q000469:parasitology; D007814:Larva / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D009132:Muscles / Q000469:parasitology; D014234:Trichinella / Q000276:immunology*; D014235:Trichinellosis / Q000276:immunology* / Q000517:prevention & control; D001503:Ursidae
chemical_list: D000911:Antibodies, Monoclonal; D007071:Immunoglobulin A, Secretory