title: Antiinflammatory activity of heat-treated Cassia alata leaf extract and its flavonoid glycoside.
abstract: Antiinflammatory activities of heat-treated Cassia alata leaf extract and kaempferol 3-O-gentiobioside (K3G) isolated from C. alata as an abundant flavonoid glycoside were studied by comparing their activities with the activities of sun-dried C. alata leaf extract. We observed strong inhibitory effects on Concanavalin A-induced histamine release from rat peritoneal exudate cells both in the extracts of heat-treated and sun-dried C. alata leaves. Furthermore, the heat-treated leaf extract exhibited stronger inhibitory effects than the effects of the sun-dried leaf extract at low concentrations in the studies of Concanavalin A-induced histamine release, 5-lipoxygenase inhibition, and also inhibition of cyclooxygenases (COX-1 and COX-2), whereas K3G showed weak inhibitory effects on Concanavalin A-induced histamine release, 5-lipoxygenase, and COX-1. No anti-hyaluronidase effect was detected in any of the materials tested.
authors.affiliation: Faculty of Pharmaceutical Sciences, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Moriyama
authors.forename: Tohru
authors.initials: T
authors.lastname: Iizuka
authors.forename: Masahiro
authors.initials: M
authors.lastname: Nagai
authors.forename: Hideki
authors.initials: H
authors.lastname: Miyataka
authors.forename: Toshio
authors.initials: T
authors.lastname: Satoh
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents; D002366:Cassia / Q000737:chemistry*; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D006636:Histamine Release / Q000187:drug effects; D006358:Hot Temperature; D007527:Isoenzymes / Q000037:antagonists & inhibitors; D044949:Kaempferols; D016859:Lipoxygenase Inhibitors; D008297:Male; D008407:Mast Cells / Q000378:metabolism; D008565:Membrane Proteins; D010529:Peritoneal Cavity / Q000166:cytology; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves; D011451:Prostaglandin-Endoperoxide Synthases; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000893:Anti-Inflammatory Agents; D005419:Flavonoids; D007527:Isoenzymes; D044949:Kaempferols; D016859:Lipoxygenase Inhibitors; D008565:Membrane Proteins; D010936:Plant Extracts; C442435:kaempferol 3-gentiobioside; D003208:Concanavalin A; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases; C496513:Ptgs1 protein, rat