title: Analytical method for the determination of the aminoglycoside gentamicin in hospital wastewater via liquid chromatography-electrospray-tandem mass spectrometry.
abstract: A method for the determination of gentamicin residues in hospital wastewater has been developed using kanamycin as a surrogate standard. The method consists of solid-phase extraction (SPE) and detection by ion-pair chromatography with electrospray tandem mass spectrometry (LC-ES-tandem MS). The SPE was performed on a weak cation exchanger. Filtration should be avoided in the sample preparation, otherwise a significant loss of gentamicin occurs. Chromatographic separation on a C18-column was achieved using a ternary eluent containing methanol, water and 1(-1) heptafluorobutyric acid solution. Mean relative recoveries of the analytes in hospital wastewater varied between 107 and 111%. The limit of quantification (LOQ) was 0.20 microg l(-1) in hospital wastewater. Gentamicin was found in native hospital wastewater in a concentration range between 0.4 and 7.6 microg l(-1).
authors.affiliation: ESWE-Institute for Water Research and Water Technology, Soehnleinstrasse 158, D-65201 Wiesbaden, Germany.
authors.forename: Dirk
authors.initials: D
authors.lastname: Löffler
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Ternes
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000737:chemistry; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005839:Gentamicins / Q000032:analysis* / Q000737:chemistry; D006761:Hospitals; D007220:Industrial Waste; D008969:Molecular Sequence Data; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins; D007220:Industrial Waste; D014874:Water Pollutants, Chemical