title: Neural basis of orexigenic effects of ghrelin acting within lateral hypothalamus.
abstract: Ghrelin stimulates feeding when administered centrally and peripherally. The lateral hypothalamus (LH) is thought to mediate ghrelin-induced hyperphagia. Thus, we examined central mechanisms underlying feeding generated by LH ghrelin. We determined that 0.3nmol of LH-injected ghrelin was the lowest dose increasing food consumption and it induced Fos immunoreactivity (IR; a marker of neuronal activation) in feeding-related brain areas, including the hypothalamic paraventricular, arcuate, and dorsomedial nuclei, amygdala, and nucleus of the solitary tract. Also, LH ghrelin induced Fos IR in LH orexin neurons. We conclude that the LH, as part of larger central circuitry, integrates orexigenic properties of ghrelin.
authors.affiliation: Veterans Affairs Medical Center, Research Service 151, Minneapolis, MN 55417, USA.
authors.forename: Pawel K
authors.initials: PK
authors.lastname: Olszewski
authors.forename: Dehong
authors.initials: D
authors.lastname: Li
authors.forename: Martha K
authors.initials: MK
authors.lastname: Grace
authors.forename: Charles J
authors.initials: CJ
authors.lastname: Billington
authors.forename: Catherine M
authors.initials: CM
authors.lastname: Kotz
authors.forename: Allen S
authors.initials: AS
authors.lastname: Levine
mesh_terms: D000679:Amygdala / Q000187:drug effects / Q000473:pathology; D000818:Animals; D001921:Brain / Q000473:pathology; D002352:Carrier Proteins / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D054439:Ghrelin; D007031:Hypothalamus / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins; D008024:Ligands; D008297:Male; D009474:Neurons / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism; D016761:Oncogene Proteins v-fos / Q000378:metabolism; D064446:Orexin Receptors; D000068797:Orexins; D036361:Peptide Hormones / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D043562:Receptors, G-Protein-Coupled / Q000737:chemistry; D054440:Receptors, Ghrelin; D018013:Receptors, Neuropeptide; D013997:Time Factors
chemical_list: D002352:Carrier Proteins; D054439:Ghrelin; D047908:Intracellular Signaling Peptides and Proteins; D008024:Ligands; D009479:Neuropeptides; D016761:Oncogene Proteins v-fos; D064446:Orexin Receptors; D000068797:Orexins; D036361:Peptide Hormones; D043562:Receptors, G-Protein-Coupled; D054440:Receptors, Ghrelin; D018013:Receptors, Neuropeptide