[Influence of anoxia/reoxygenation on immunofunction of endothelial cells and effect of intervention with yisheng injection on it].
OBJECTIVE
To explore the influence of ischemia/reperfusion (anoxia/reoxygenation) on immunofunction of endothelial cells (ECs) and effect of intervention with Yisheng injection (YSI, a pure natural medicine) on it.


METHODS
Model of ECs induced by anoxia/reoxygenation was established to mimic ECs ischemia/reperfusion injury in vivo with human umbilical vein endothelial cell line ECV304. Then YSI was used to intervene the anoxia/reoxygenation process. Nuclear transcriptional factor-kappa B (NF-kappa B) was exhibited by fluorescent staining, HLA-ABC, HLA-DR, CD86 and CD54 were detected by flow cytometry. Mixed endothelial cell-lymphocyte reaction (MELR) was conducted to examine the proliferation of lymphocyte, production of IL-2 and percentage of apoptotic lymphocyte.


RESULTS
Anoxia/reoxygenation made the ECV304 cell became round, shrunk and abscised, with increased plasma NF-kappa B, and changed from positive cytoplasm to positive nucleus. HLA-ABC, HLA-DR and CD86 on surface of cells increased but CD54 showed unchanged. MELR showed the incorporation of 3H-TdR and production of IL-2 increased significantly and the percentage of apoptotic lymphocyte decreased. After YSI intervention, the ECV304 cell shaped recovered, NF-kappa B expression didn't down-regulated, but the percentage of positive cells decreased, changed to positive dominant. Besides, reversal changes were shown in other parameters.


CONCLUSIONS
Anoxia/reoxygenation influences some important immune related molecules in ECV304 cells, YSI could antagonizing these influences to maintain the immune function of endothelial cells in a relative normal manner.
Jiang
Hong-mei
HM
Lab of Transplant Engineering and Immunology, West China Hospital, Sichuan University, Chengdu 610041. jhmzq@yahoo.com.cn
Li
Sheng-fu
SF
Li
You-ping
YP
D015687:Cell Hypoxia; D002460:Cell Line; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology* / Q000276:immunology; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism; D010100:Oxygen / Q000494:pharmacology; D008517:Phytotherapy; D015427:Reperfusion Injury / Q000276:immunology*; D014471:Umbilical Veins / Q000166:cytology
D004365:Drugs, Chinese Herbal; D016328:NF-kappa B; D010100:Oxygen