title: Invasion of Salmonella enteritidis in avian intestinal epithelial cells in vitro is influenced by short-chain fatty acids.
abstract: Fermentation reactions in the caeca of chickens, the predominant place for Salmonella colonization, result in high concentrations of short-chain fatty acids (SCFA). Thus Salmonella bacteria are in close contact with SCFA during their life cycle. A study was carried out to analyse the effects of SCFA on invasion of Salmonella enteritidis in an avian intestinal epithelial cell line. Preincubation of S. enteritidis for 4 h in growth media supplemented with various concentrations of propionate or butyrate resulted in decreased invasion compared to bacteria, preincubated in nonsupplemented media, and to bacteria, preincubated in media supplemented with formate or acetate. Incubation of the S. enteritidis bacteria in media supplemented with mixtures of SCFA mimicking the in vivo caecal concentrations resulted in increased invasion compared with butyrate-exposed bacteria, but equal invasion compared with nonexposed bacteria. Increasing the butyrate concentration in these mixtures did not modify invasion compared with the original mixtures.
authors.affiliation: Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Ghent, Belgium. filip.vanimmerseel@rug.ac.be
authors.forename: F
authors.initials: F
authors.lastname: Van Immerseel
authors.forename: J
authors.initials: J
authors.lastname: De Buck
authors.forename: F
authors.initials: F
authors.lastname: Pasmans
authors.forename: P
authors.initials: P
authors.lastname: Velge
authors.forename: E
authors.initials: E
authors.lastname: Bottreau
authors.forename: V
authors.initials: V
authors.lastname: Fievez
authors.forename: F
authors.initials: F
authors.lastname: Haesebrouck
authors.forename: R
authors.initials: R
authors.lastname: Ducatelle
mesh_terms: D000818:Animals; D002460:Cell Line; D002645:Chickens; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000382:microbiology*; D005232:Fatty Acids, Volatile / Q000494:pharmacology*; D005516:Food Microbiology; D012477:Salmonella enteritidis / Q000254:growth & development / Q000472:pathogenicity*; D014774:Virulence
chemical_list: D005232:Fatty Acids, Volatile