[Analysis of CD117 expression on leukemia cells].
OBJECTIVE
To explore the expression of CD(117) on different types of leukemia and its significance.


METHODS
CD(117) expression was analysed by three-color flow cytometry with CD(45)/SSC gating strategy.


RESULTS
CD(117) was expressed in 68% of acute myeloid leukemia (AML) and 80% of chronic myeloid leukemia in blast crisis (CML-BC) cases, but in only 2% of acute lymphoblastic leukemia cases. CD(117) was negative in CML in chronic phase and chronic lymphocytic leukemia. Cases in AML, CD(117) was expressed in 72% of M(0)/M(1), 88% of M(2), 50% of M(4), 75% of M(5a), 100% of M(6) and 100% of M(7) cases but was less expressed in M(3) (39%) and M(5b) (29%) cases. Expression of CD(117) was more frequent than CD(34) and HLA-DR in M(3) and some M(2) cases. There was a significant inverse relationship between CD(117) and CD(14) expression in AML.


CONCLUSIONS
Analysis of CD(117) expression may be of help in distinguishing myeloid from lymphoid leukemia and leukemia clone from normal cells.
Shen
Hong-qiang
HQ
Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
Tang
Yong-min
YM
Yang
Shi-long
SL
Qian
Bo-qin
BQ
Song
Hua
H
Shi
Shu-wen
SW
Xu
Wei-qun
WQ
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D003937:Diagnosis, Differential; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007223:Infant; D007945:Leukemia, Lymphoid / Q000175:diagnosis / Q000378:metabolism*; D007951:Leukemia, Myeloid / Q000175:diagnosis / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D019009:Proto-Oncogene Proteins c-kit / Q000032:analysis / Q000096:biosynthesis*
D019009:Proto-Oncogene Proteins c-kit