A case of pulmonary aspergilloma molecular biological identification and typing of the isolates from antemortem sputa and autopsy fungus ball.
Filamentous fungi were isolated from antemortem sputum and an autopsy fungus ball of the lung in a case of aspergilloma. Both of the isolates were analyzed for the sequences of species or strain-specific nuclear ribosomal DNA (partial 28S and ITS1 regions), and were identified as Aspergillus fumigatus. The molecular biological technique saved time and is thought to be a powerful tool in the accurate diagnosis of pulmonary fungal infection to assure effective treatment.
Sugita
C
C
First Department of Internal Medicine, Tokyo Woman's Medical University, Tokyo, Japan.
Makimura
K
K
Murakami
A
A
Murai
Y
Y
Yamaguchi
H
H
Nagai
A
A
D001228:Aspergillosis / Q000382:microbiology* / Q000473:pathology; D001232:Aspergillus fumigatus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001344:Autopsy; D001483:Base Sequence; D021903:DNA, Ribosomal Spacer / Q000032:analysis*; D006801:Humans; D008168:Lung / Q000382:microbiology / Q000473:pathology; D008172:Lung Diseases, Fungal / Q000382:microbiology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D012339:RNA, Ribosomal, 28S / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013183:Sputum / Q000382:microbiology
D021903:DNA, Ribosomal Spacer; D012339:RNA, Ribosomal, 28S