title: Mechanism of anticlastogenicity of Agaricus blazei Murill mushroom organic extracts in wild type CHO (K(1)) and repair deficient (xrs5) cells by chromosome aberration and sister chromatid exchange assays.
abstract: Agaricus blazei Murill is a medicinal mushroom native to Brazil. The present work assessed the clastogenic and anticlastogenic potential of organic extracts (ethanol and chloroform/methanol) from the lineage AB97/11 in chinese hamster CHO-K(1) (wild type) and CHO-xrs5 (repair deficient) cells using the chromosome aberration (CA) and sister chromatid exchange (SCE) assays. In these experimental conditions were observed: (a) anticlastogenic effect at concentrations of 0.06 and 0.09% of the EtOH extract and at the 0.03 and 0.06% concentrations of the C/MetOH extract in CHO-K(1); (b) absence of protector effect on CHO-xrs5 cells; and (c) absence of protector effect in the SCE assay. These results indicate that organic extracts of A. blazei lineage AB97/11 present bio-antimutagenic type protective activity.
authors.affiliation: Departamento de Biologia Geral, Centro de Ciencias Biologicas (CCB), Universidade Estadual de Londrina, Campus Universitário, Cx. Postal 6001, CEP 86051-990 PR Londrina, Brazil.
authors.forename: R C
authors.initials: RC
authors.lastname: Luiz
authors.forename: B Q
authors.initials: BQ
authors.lastname: Jordão
authors.forename: A F
authors.initials: AF
authors.lastname: da Eira
authors.forename: L R
authors.initials: LR
authors.lastname: Ribeiro
authors.forename: M S
authors.initials: MS
authors.lastname: Mantovani
mesh_terms: D000364:Agaricus / Q000737:chemistry*; D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D016466:CHO Cells; D002869:Chromosome Aberrations; D006224:Cricetinae; D009152:Mutagenicity Tests; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal; D012854:Sister Chromatid Exchange
chemical_list: D016587:Antimutagenic Agents; D010936:Plant Extracts