Features of serum beta-lipoprotein electrophoretogram in patients with coronary heart disease.
OBJECTIVE
To investigate the changes in serum beta-lipoprotein (beta Lp) electrophoretogram in the patients with coronary heart disease (CHD).


METHODS
This study included 36 CHD patients, 28 non-CHD patients and 8 normal control subjects, from whom serum beta Lps (prestained with Sudan Black B) were isolated by means of the first double-gradient polyacrylamide gel electrophoresis (f-DGPAGE). The secondary DGPAGE was performed for isolating beta Lps into 4 subfractions (beta Lp1-4) in order to complete the quantitative analysis of beta Lp electrophoretogram. A parallel test to examine the serum concentration of low-density lipoprotein cholesterol (LDLc) was also carried out.


RESULTS
The relative amount of beta Lp1 was significantly elevated in CHD patients (53.4+/-10.0%) as compared with normal subjects (39.3+/-6.7%) and non-CHD patients (42.8+/-6.8%, P < 0.05 for both comparisons) regardless of serum LDLc concentrations, and beta Lp1 content between the latter 2 groups showed no significant difference.


CONCLUSIONS
Serum beta Lp subfraction imbalance evidenced by increased beta Lp1 level characterizes the patients with CHD.
Chen
Yun-qin
YQ
Electrocardiology Research Center, Department of Basic Science, First Military Medical University, Guangzhou 510515, China. SLPG@fimmu.edu.cn
Yin
Jia
J
Zhang
Min
M
Yin
Bing-sheng
BS
D000328:Adult; D000368:Aged; D001797:Blood Protein Electrophoresis; D008078:Cholesterol, LDL / Q000097:blood; D003327:Coronary Disease / Q000097:blood*; D005260:Female; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood*; D008297:Male; D008875:Middle Aged
D008078:Cholesterol, LDL; D008077:Lipoproteins, LDL