Design of acoustic cell settler for filtering and recycling microbial cells.
An acoustic cell settler (ACS) using ultrasound at cells of 3 MHz was used to recycle Saccharomyces cerevisiae in a fermenter. The locations of both the inlet and outlet in the acoustic cell settler, which have a relatively long distance between the transducer and reflector, were optimized. A tilted settler was designed to make up for the defect in the horizontal ACS, which has a low recovery ratio. The tilted ACS gave a recovery ratio of yeast cells of about 5 during the most period of operation, which was twice that of the horizontal ACS.
Hwang
Sung-Ho
SH
Department of Biological Engineering, ERC for the Advanced Bioseparation Technology, Inha University, Incheon 402-751, Korea.
Koo
Yoon-Mo
YM
D002469:Cell Separation; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005374:Filtration / Q000295:instrumentation* / Q000379:methods*; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods; D011786:Quality Control; D012441:Saccharomyces cerevisiae / Q000302:isolation & purification* / Q000502:physiology / Q000528:radiation effects*; D014465:Ultrasonics