title: Association of L protein and in vitro tomato spotted wilt virus RNA-dependent RNA polymerase activity.
abstract: We have previously described an in vitro assay for RNA-dependent RNA polymerase activity in virions of tomato spotted wilt virus (TSWV). Here we report antibody inhibition of virion-associated RNA synthesis in vitro with an L-protein-specific polyclonal antibody raised against the carboxy-terminus of the L protein. In contrast, RNA synthesis was not inhibited by a heterologous antiserum and was unaffected by antiserum raised against an internal portion of the L protein. Our results directly associate the TSWV L protein, the putative viral polymerase, with RNA synthesis functions in vitro.
authors.affiliation: Department of Plant Pathology, University of Wisconsin-Madison, 53706, USA.
authors.forename: Elisabeth J
authors.initials: EJ
authors.lastname: Chapman
authors.forename: Pierre
authors.initials: P
authors.lastname: Hilson
authors.forename: Thomas L
authors.initials: TL
authors.lastname: German
mesh_terms: D000914:Antibodies, Viral / Q000276:immunology; D003001:Cloning, Molecular; D018076:DNA, Complementary; D018551:Solanum lycopersicum / Q000821:virology; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000096:biosynthesis*; D012324:RNA-Dependent RNA Polymerase / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D017865:Tospovirus / Q000201:enzymology* / Q000235:genetics; D014764:Viral Proteins / Q000276:immunology / Q000378:metabolism*; D014771:Virion / Q000235:genetics / Q000378:metabolism
chemical_list: D000914:Antibodies, Viral; D018076:DNA, Complementary; D012367:RNA, Viral; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase