title: Temporal variations of adhesion molecules and matrix metalloproteinases in the course of MS.
abstract: Thirty patients with clinically isolated syndromes (CIS) were evaluated at the onset of neurological symptoms and when they developed clinically definite MS (CDMS). Surface expression of LFA-1alpha, VLA-4 and intercellular adhesion molecule-1 (ICAM-1) on PBMC and CSF cells was evaluated using flow cytometry. Serum and CSF concentrations of soluble vascular cell adhesion molecules-1 (VCAM-1), ICAM-1 and E-Selectin, as well as MMP-9 and MMP-2 serum concentrations were assayed using ELISA. Surface expression of LFA-1alpha and VLA-4 molecules on peripheral blood and CSF T cells and monocytes from CIS and CDMS was significantly increased compared with control subjects. Moreover, LFA-1alpha and VLA-4 expression was significantly higher in patients who developed CDMS compared with those with CIS. Similar changes were observed in the serum levels of MMP-9. Furthermore, patients with CIS and CDMS had significantly higher levels of CSF sVCAM and s-E-Selectin than control subjects. These data suggest that VLA-4, LFA-1alpha and MMP-9 play a leading role in the evolution of inflammatory demyelinating lesions in patients with CIS who develop CDMS.
authors.affiliation: Department of Neurology, Raúl Carrea Institute for Neurological Research (FLENI), Montañeses 2325 (1428), Buenos Aires, Argentina. jcorreale@fleni.org.ar
authors.forename: Jorge
authors.initials: J
authors.lastname: Correale
authors.forename: María de los Milagros
authors.initials: Mde L
authors.lastname: Bassani Molinas
mesh_terms: D000328:Adult; D015815:Cell Adhesion Molecules / Q000096:biosynthesis* / Q000097:blood / Q000134:cerebrospinal fluid; D002462:Cell Membrane / Q000201:enzymology / Q000378:metabolism; D005260:Female; D006801:Humans; D039041:Integrin alpha4beta1 / Q000096:biosynthesis / Q000097:blood; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis / Q000097:blood / Q000134:cerebrospinal fluid; D008137:Longitudinal Studies; D016169:Lymphocyte Function-Associated Antigen-1 / Q000096:biosynthesis / Q000097:blood / Q000134:cerebrospinal fluid; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis / Q000097:blood; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis / Q000097:blood; D020782:Matrix Metalloproteinases / Q000096:biosynthesis* / Q000097:blood; D008875:Middle Aged; D009000:Monocytes / Q000201:enzymology / Q000378:metabolism; D009103:Multiple Sclerosis / Q000097:blood / Q000134:cerebrospinal fluid / Q000201:enzymology* / Q000378:metabolism*; D012995:Solubility; D016176:T-Lymphocyte Subsets / Q000201:enzymology / Q000378:metabolism; D013997:Time Factors; D015854:Up-Regulation
chemical_list: D015815:Cell Adhesion Molecules; D039041:Integrin alpha4beta1; D016169:Lymphocyte Function-Associated Antigen-1; D018799:Intercellular Adhesion Molecule-1; D020782:Matrix Metalloproteinases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9