Axonal plasticity and functional recovery after spinal cord injury in mice deficient in both glial fibrillary acidic protein and vimentin genes.
The lack of axonal regeneration in the injured adult mammalian spinal cord leads to permanent functional disabilities. The inability of neurons to regenerate their axon is appreciably due to an inhospitable environment made of an astrocytic scar. We generated mice knock-out for glial fibrillary acidic protein and vimentin, the major proteins of the astrocyte cytoskeleton, which are upregulated in reactive astrocytes. These animals, after a hemisection of the spinal cord, presented reduced astroglial reactivity associated with increased plastic sprouting of supraspinal axons, including the reconstruction of circuits leading to functional restoration. Therefore, improved anatomical and functional recovery in the absence of both proteins highlights the pivotal role of reactive astrocytes in axonal regenerative failure in adult CNS and could lead to new therapies of spinal cord lesions.
Menet
V
V
Institut National de la Santé et de la Recherche Médicale U 583, Université de Montpellier II, Place E. Bataillon, F-34095 Montpellier Cedex 05, France.
Prieto
M
M
Privat
A
A
Giménez y Ribotta
M
M
D000818:Animals; D001253:Astrocytes / Q000502:physiology; D001369:Axons / Q000502:physiology; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000172:deficiency* / Q000235:genetics / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D018345:Mice, Knockout; D009043:Motor Activity; D009416:Nerve Regeneration; D009473:Neuronal Plasticity; D013119:Spinal Cord Injuries / Q000473:pathology / Q000503:physiopathology* / Q000628:therapy; D014746:Vimentin / Q000172:deficiency* / Q000235:genetics / Q000502:physiology
D005904:Glial Fibrillary Acidic Protein; D014746:Vimentin