Pregnancy outcome in mares following insemination deep in the uterine horn with low numbers of sperm selected by glass wool/Sephadex filtration, Percoll separation or absolute number.
Mares were inseminated deep in the uterine horn with 25 million sperm selected by glass wool/Sephadex (GWS) filtration, Percoll separation (PS) or absolute number (AN). Deep-horn insemination using a low-volume, smooth tipped, flexible pipette/catheter delivery system allowed more efficient use of stallion sperm and reduced post-breeding uterine reaction in mares. Mares were pregnant in 15/30, 13/30 and 10/30 cycles for GWS, PS and AN selection methods, respectively. Sperm selection method did not effect pregnancy outcome (P=0.422). However, sperm selected for deep-horn insemination by filtration through a glass wool/Sephadex column tended to improve fertility over simply using an absolute number of sperm (P=0.105).
Nie
Gary J
GJ
Department of Clinical Sciences, College of Veterinary Medicine, Auburn University, AL 36849-5522, USA. niegary@earthlink.net
Johnson
Kristina E
KE
Wenzel
James G W
JG
D000818:Animals; D001947:Breeding; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D003911:Dextrans; D005260:Female; D005898:Glass; D006736:Horses; D007315:Insemination, Artificial / Q000379:methods / Q000662:veterinary*; D008297:Male; D011205:Povidone; D011247:Pregnancy; D011256:Pregnancy Outcome / Q000662:veterinary*; D012822:Silicon Dioxide; D013076:Sperm Count; D013094:Spermatozoa / Q000166:cytology
D003911:Dextrans; C016039:Percoll; D012822:Silicon Dioxide; C025614:sephadex; D011205:Povidone