title: Neutrophil and eosinophil participation in atopic and vernal keratoconjunctivitis.
abstract: OBJECTIVE
  A retrospective study was conducted at three centers to examine the participation of neutrophils and eosinophils in the inflammatory processes associated with atopic keratoconjunctivitis (AKC) and vernal keratoconjunctivitis (VKC).
  
  
  METHODS
  Conjunctival specimens were obtained from four patients with AKC, six with VKC, and five normal subjects. Indirect immunofluorescent staining was used to localize neutrophil elastase (NE) and eosinophil granule major basic protein (MBP) in serial sections of all specimens.
  
  
  RESULTS
  Specimens from both AKC and VKC patients revealed extracellular deposition of NE and MBP. Control specimens showed no or minimal extracellular NE, and no MBP. Statistical analysis demonstrated significantly greater extracellular MBP deposition in AKC specimens compared to controls (p = 0.009), and VKC specimens showed significantly greater staining for both extracellular MBP (p = 0.005) and NE (p = 0.006).
  
  
  CONCLUSIONS
  These results suggest that neutrophils, in addition to eosinophils, play an active role in the pathogenesis of AKC and VKC as evidenced by the extracellular deposition of their specific granule proteins.
authors.affiliation: Department of Ophthalmology and Visual Sciences, University of Texas Medical Branch, 700 University Boulevard, Galveston, TX 77555-1106, USA. drtroc@aol.com
authors.forename: S D
authors.initials: SD
authors.lastname: Trocme
authors.forename: K M
authors.initials: KM
authors.lastname: Leiferman
authors.forename: T
authors.initials: T
authors.lastname: George
authors.forename: S
authors.initials: S
authors.lastname: Bonini
authors.forename: C Stephen
authors.initials: CS
authors.lastname: Foster
authors.forename: E E
authors.initials: EE
authors.lastname: Smit
authors.forename: S K
authors.initials: SK
authors.lastname: Sra
authors.forename: L R
authors.initials: LR
authors.lastname: Grabowski
authors.forename: C H
authors.initials: CH
authors.lastname: Dohlman
mesh_terms: D000328:Adult; D001798:Blood Proteins / Q000378:metabolism*; D003233:Conjunctivitis, Allergic / Q000378:metabolism*; D047091:Eosinophil Granule Proteins; D004804:Eosinophils / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D019272:Leukocyte Elastase / Q000378:metabolism*; D008297:Male; D009504:Neutrophils / Q000378:metabolism*; D012189:Retrospective Studies; D012260:Ribonucleases
chemical_list: D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D018836:Inflammation Mediators; D012260:Ribonucleases; D019272:Leukocyte Elastase