title: Effects of biomass accumulation on microbially enhanced dissolution of a PCE pool: a numerical simulation.
abstract: Recent studies have shown that dechlorinating bacteria can accelerate the dissolution rate of dense, nonaqueous phase liquids (DNAPLs) containing tetrachloroethene (PCE). We present an advection-dispersion-reaction model for a two-dimensional domain, with groundwater flowing over a pool of free-product PCE. PCE is converted to cis-1,2-dichloroethene (cDCE) and toxicity due to PCE or cDCE is neglected. We adopt previously published correlations relating biomass concentrations and hydraulic conductivity, accounting for biofilm growth and plug-like growth. The system of coupled equations is solved numerically. The high biotransformation rate of PCE increases the concentration gradient of PCE at the water-DNAPL interface, enhancing dissolution. The higher the electron donor (ED) concentration, the larger the dissolution enhancement. Based on the values of maximum specific rate we used, when the electron donor is unlimited, the active biomass accumulates adjacent to the water-NAPL interface and microbial reactions can significantly enhance the pool dissolution. The resulting steady-state dissolution rate can be approximated by a half-order solution when zero-order kinetics are suitable for representing the microbial reaction. However, bioclogging may significantly reduce local hydraulic conductivity; thus, it decreases the flow near the water-DNAPL interface, decreasing dissolution. When the ED is the limiting factor, active biomass accumulates away from the interface. This creates a no-flow zone between the active biomass and the interface. The enlargement of the no-flow zone, due to the donor limitation, diminishes the concentration gradient and the flushing around the water-DNAPL interface. Such adverse impacts may significantly decrease the enhancement predicted by models that do not consider the effects of bioclogging.
authors.affiliation: Department of Civil and Environmental Engineering, Stanford University, Stanford, CA 94305-4020, USA. chumi@stanford.edu
authors.forename: M
authors.initials: M
authors.lastname: Chu
authors.forename: P K
authors.initials: PK
authors.lastname: Kitanidis
authors.forename: P L
authors.initials: PL
authors.lastname: McCarty
mesh_terms: D018533:Biomass; D002273:Carcinogens / Q000378:metabolism*; D007700:Kinetics; D008962:Models, Theoretical; D012995:Solubility; D013750:Tetrachloroethylene / Q000378:metabolism*; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D018508:Water Purification
chemical_list: D002273:Carcinogens; D014874:Water Pollutants, Chemical; D013750:Tetrachloroethylene