Fluorescence-enhanced optical imaging in large tissue volumes using a gain-modulated ICCD camera.
A novel image-intensified charge-coupled device (ICCD) imaging system has been developed to perform 3D fluorescence tomographic imaging in the frequency-domain using near-infrared contrast agents. The imager is unique since it (i) employs a large tissue-mimicking phantom, which is shaped and sized to resemble a female breast and part of the extended chest-wall region, and (ii) enables rapid data acquisition in the frequency-domain by using a gain-modulated ICCD camera. Diffusion model predictions are compared to experimental measurements using two different referencing schemes under two different experimental conditions of perfect and imperfect uptake of fluorescent agent into a target. From these experimental measurements, three-dimensional images of fluorescent absorption were reconstructed using a computationally efficient variant of the approximate extended Kalman filter algorithm. The current work represents the first time that 3D fluorescence-enhanced optical tomographic reconstructions have been achieved from experimental measurements of the time-dependent light propagation on a clinically relevant breast-shaped tissue phantom using a gain-modulated ICCD camera.
Godavarty
Anuradha
A
Department of Chemical Engineering, Texas A&M University, College Station, TX 77843-3122, USA.
Eppstein
Margaret J
MJ
Zhang
Chaoyang
C
Theru
Sangeeta
S
Thompson
Alan B
AB
Gurfinkel
Michael
M
Sevick-Muraca
Eva M
EM
D055592:Biophysical Phenomena; D001703:Biophysics; D001940:Breast / Q000033:anatomy & histology; D001941:Breast Diseases / Q000175:diagnosis; D004867:Equipment Design; D005260:Female; D005453:Fluorescence; D006801:Humans; D007091:Image Processing, Computer-Assisted; D055095:Optics and Photonics / Q000295:instrumentation; D019047:Phantoms, Imaging; D014054:Tomography / Q000295:instrumentation*