Q-Gene: processing quantitative real-time RT-PCR data.
CONCLUSIONS
Q-Gene is an application for the processing of quantitative real-time RT-PCR data. It offers the user the possibility to freely choose between two principally different procedures to calculate normalized gene expressions as either means of Normalized Expressions or Mean Normalized Expressions. In this contribution it will be shown that the calculation of Mean Normalized Expressions has to be used for processing simplex PCR data, while multiplex PCR data should preferably be processed by calculating Normalized Expressions. The two procedures, which are currently in widespread use and regarded as more or less equivalent alternatives, should therefore specifically be applied according to the quantification procedure used.


BACKGROUND
Web access to this program is provided at http://www.biotechniques.com/softlib/qgene.html
Simon
Perikles
P
Section for Neurobiology of the Eye, University Eye Hospital Tuebingen, Calwerstr. 7/1, 72076 Tuebingen, Germany. perikles@uni-tuebingen.de
D000465:Algorithms; D020869:Gene Expression Profiling / Q000379:methods*; D009862:Online Systems; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D016415:Sequence Alignment / Q000379:methods*; D017423:Sequence Analysis, RNA / Q000379:methods*; D012984:Software