[The effect of phorbol ester in a wide range of concentrations on the lipid structure of microsome membranes of tumor cells in vitro].
The action of 12-O-tetradecanoyl-13-acetate (TPA) in vitro in a wide range of concentration from 10(-3) mol/l down to ultra-low doses 10(-23) mol/l and dilution 10(-24) mol/l on the microsome membranes isolated from tumor--Ehrlich ascite carcinoma (EAC) has been studied by ESR-method using two spin probes: 5- and 16-doxyl stearates (5- and 16-DS) localized in the different regions of lipid bilayer. From the ESR spectra obtained it was calculated the following parameters: an order of the long axis 5-DS (S) related to order of the fatty acids chains in the lipid bilayer; two rotation correlation times (Tc1 and Tc2) of 16-DC to estimate a microviscosity value and structural-sensitive ones. It was found the stage changes of all these parameters (increase and decrease) as compared with control level (the membranes untreated by TPA) depending on TPA concentration into the range of 10(-3)-10(-24) mol/l; in particular, the most significant shape changes of structural-sensitive parameters have been observed at TPA doses below 10(-16) mol/l. It is concluded that tumor membranes are very sensitive to TPA action in vitro in a wide range of concentration included ultra-low doses.
Mal'tseva
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Emanuel Institute of Biochemical Physics, Russian Academy of Russia, Moscow, 119991 Russia. emal@sky.chph.ras.ru
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D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000737:chemistry; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism; D003497:Cyclic N-Oxides / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004578:Electron Spin Resonance Spectroscopy; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry; D051379:Mice; D008815:Mice, Inbred Strains; D008861:Microsomes / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D013113:Spin Labels; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D003497:Cyclic N-Oxides; D008055:Lipids; D013113:Spin Labels; C032363:16-nitroxystearic acid; D013755:Tetradecanoylphorbol Acetate