Capsicum annuum dehydrin, an osmotic-stress gene in hot pepper plants.
Osmotic stress-related genes were selected from an EST database constructed from 7 cDNA libraries from different tissues of the hot pepper. A full-length cDNA of Capsicum annuum dehydrin (Cadhn), a late embryogenesis abundant (lea) gene, was selected from the 5' single pass sequenced cDNA clones and sequenced. The deduced polypeptide has 87% identity with potato dehydrin C17, but very little identity with the dehydrin genes of other organisms. It contains a serine-tract (S-segment) and 3 conserved lysine-rich domains (K-segments). Southern blot analysis showed that 2 copies are present in the hot pepper genome. Cadhn was induced by osmotic stress in leaf tissues as well as by the application of abscisic acid. The RNA was most abundant in green fruit. The expression of several osmotic stress-related genes was examined and Cadhn proved to be the most abundantly expressed of these in response to osmotic stress.
Chung
Eunsook
E
Plant Genomics Laboratory, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-600, Korea.
Kim
Soo-Yong
SY
Yi
So Young
SY
Choi
Doil
D
D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002212:Capsicum / Q000235:genetics*; D003681:Dehydration; D015723:Gene Library; D017343:Genes, Plant; D016905:Gram-Negative Bacterial Infections / Q000378:metabolism; D008969:Molecular Sequence Data; D009997:Osmotic Pressure; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics*; D014974:Xanthomonas
D010940:Plant Proteins; C066246:late embryogenesis abundant protein, plant; D000040:Abscisic Acid