title: Expression of estrogen receptor alpha and beta genes in the mediobasal hypothalamus, pituitary and ovary during the canine estrous cycle.
abstract: Estrogen receptor alpha (ERalpha) and ERbeta mRNA levels were measured in the mediobasal hypothalamus, the anterior pituitary and the ovary of beagle bitches at various stages of the estrous cycle. With polymerase chain reaction analysis we detected ERbeta gene transcripts in all tissue samples. The levels of hypothalamic and pituitary ERalpha and beta mRNAs increased from mid anestrus to proestrus and declined thereafter. In the ovary, ERalpha mRNA levels increased from proestrus to diestrus and were positively correlated with plasma progesterone levels (r=0.62, P<0.01), whereas ERbeta mRNA levels increased from mid anestrus to proestrus and were positively correlated with plasma estradiol-17beta levels (r=0.73, P<0.001). These results suggest that the rise in hypothalamic and pituitary ERalpha and beta mRNAs is associated with termination of anestrus, and that increases in ovarian ERalpha and beta mRNAs may be involved in initiating development of the follicle or corpora lutea.
authors.affiliation: Laboratory of Cell Pathobiology, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, 599-8531 Osaka, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Hatoya
authors.forename: Ryuzo
authors.initials: R
authors.lastname: Torii
authors.forename: Daijiro
authors.initials: D
authors.lastname: Kumagai
authors.forename: Kikuya
authors.initials: K
authors.lastname: Sugiura
authors.forename: Noritoshi
authors.initials: N
authors.lastname: Kawate
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Tamada
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Sawada
authors.forename: Toshio
authors.initials: T
authors.lastname: Inaba
mesh_terms: D000818:Animals; D004285:Dogs; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D030762:Estrous Cycle; D005260:Female; D007031:Hypothalamus / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism*; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D012333:RNA, Messenger; D011960:Receptors, Estrogen