Altered immunoreactive levels of G proteins in peripheral mononuclear cells of patients with anorexia nervosa and bulimia nervosa.
Alterations of cellular G proteins have been implicated in the pathophysiology of some psychiatric disorders. So far, no study assessed G protein function in anorexia nervosa (AN) and bulimia nervosa (BN). Therefore, we measured immunoreactive levels of G(alpha s), G(alpha i), G(alpha q/11) and G(beta) protein subunits in mononuclear leukocytes of 71 drug-free women, including 25 subjects with AN, 26 individuals with BN and 20 healthy controls. As compared to healthy women, anorexic patients exhibited significantly increased levels of G(alpha i) and G(beta) proteins, while bulimic patients had significantly increased levels of G(alpha s), G(alpha i) and G(beta) proteins. Immunoreactive levels of peripheral G protein subunits were not significantly correlated with demographic or nutritional parameters. These findings, although obtained in peripheral blood cells, may suggest a derangement of G protein-mediated signal transduction in the pathophysiology of eating disorders.
Monteleone
P
P
Department of Psychiatry, University of Naples S.U.N., Naples, Italy. monteri@tin.it
Di Lieto
A
A
Martiadis
V
V
Pannuto
M
M
Maj
M
M
D000293:Adolescent; D000328:Adult; D000856:Anorexia Nervosa / Q000097:blood* / Q000503:physiopathology; D015153:Blotting, Western; D002032:Bulimia / Q000097:blood* / Q000503:physiopathology; D005260:Female; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000097:blood*; D043802:GTP-Binding Protein alpha Subunits, Gq-G11 / Q000097:blood*; D019205:GTP-Binding Protein alpha Subunits, Gs / Q000097:blood*; D044387:GTP-Binding Protein beta Subunits / Q000097:blood*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry*; D012720:Severity of Illness Index; D015398:Signal Transduction
D044387:GTP-Binding Protein beta Subunits; D019206:GTP-Binding Protein alpha Subunits, Gi-Go; D043802:GTP-Binding Protein alpha Subunits, Gq-G11; D019205:GTP-Binding Protein alpha Subunits, Gs