title: Role of adenohypophyseal mixed cell-follicles in age estimation.
abstract: In this study we used paraffin-embedded human pituitary obtained from 248 autopsy cases and identified mixed cell follicles by the immunohistochemical method. We examined the number and size of the mixed cell follicles, and the ratio of each component cell of these follicles, in the anterior pituitary at various age groups. The number of follicles increased with age, and the size of the follicles also tended to enlarge with age. Statistical analysis showed that a high correlation existed between age and the number or the size of the mixed cell-follicles formed by various adenohypophyseal cells. In addition, when the proportions of the different cell types that formed the follicles were examined, sex differences were observed with aging for the GH cells, the PRL cells, and the gonadotroph (GTH) cells, while no changes were observed with aging in both men and women for the ACTH cells and TSH cells. These results indicate that the number, size, and ratio of each component cell of follicles in the anterior pituitary are adequately applicable for the purpose of age estimation in routine forensic medicine.
authors.affiliation: Department of Legal Medicine, Graduate School of Medicine and Dentistry, Okayama University, Okayama 700-8558, Japan.
authors.forename: Takaki
authors.initials: T
authors.lastname: Ishikawa
authors.forename: Satoru
authors.initials: S
authors.lastname: Miyaishi
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Tachibana
authors.forename: Yuji
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Hideo
authors.initials: H
authors.lastname: Ishizu
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000375:Aging / Q000502:physiology; D002452:Cell Count; D005260:Female; D005554:Forensic Medicine; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D010903:Pituitary Gland, Anterior / Q000166:cytology* / Q000502:physiology