title: Adeno-associated virus mediated LacZ gene transfect to cultured human iris pigment epithelium cells.
abstract: OBJECTIVE
  To study the feasibility of adeno-associated virus mediated gene transfection to cultured human iris pigment epithelium (IPE) cells in vitro.
  
  
  METHODS
  Recombinant replication deficient adeno-associated viruses (AAV) expressing LacZ gene were produced without helper virus. The LacZ gene was transduced into cultured human IPE cells.
  
  
  RESULTS
  Cultured human IPE cells stained positively anticytokeratin, The titer of rAAV-LacZ was 2.1 x 10(8) virus particles/ml, 42% cultured human IPE cells expressed beta-galactosidase 7 days after transfection and 67% after 14 days.
  
  
  CONCLUSIONS
  Recombined AAV produced without helper virus can transfer a foreign gene into human IPE cells with high efficiency in vitro.
authors.affiliation: Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Zhang
authors.forename: Shibo
authors.initials: S
authors.lastname: Tang
authors.forename: Yan
authors.initials: Y
authors.lastname: Luo
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Liang
authors.forename: Jing
authors.initials: J
authors.lastname: Ma
authors.forename: Shaofen
authors.initials: S
authors.lastname: Lin
mesh_terms: D002478:Cells, Cultured; D004274:DNA, Recombinant; D000229:Dependovirus / Q000235:genetics*; D015870:Gene Expression; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006801:Humans; D007498:Iris / Q000166:cytology / Q000378:metabolism*; D007763:Lac Operon / Q000235:genetics*; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000378:metabolism*; D014162:Transfection; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D004274:DNA, Recombinant; D001616:beta-Galactosidase