title: Human liver catalase: cloning, expression and characterization of monoclonal antibodies.
abstract: We isolated a cDNA encoding liver catalase from a human liver cDNA library. The cDNA had a high degree of sequence similarity to the corresponding enzyme from other sources. It was expressed in E. coli using the pET15b vector. The protein produced was enzymatically active after purification, and its kinetic parameters closely resembled those of other mammalian catalases. Monoclonal antibodies were generated against the purified catalase; six antibodies recognizing different epitopes were obtained, one of which inhibited the enzyme. The cross reactions of the antibodies with brain catalases from human and other mammalian tissues were investigated, and all the immunoreactive bands obtained on Western blots had molecular masses of about 58 kDa. Similarly fractionated extracts of several mammalian cell lines all gave a single band of molecular mass 58 kDa. These results indicate that mammalian livers and human cell lines contain only one major type of immunologically reactive catalase, even though some of catalases have been previously reported to differ in certain properties.
authors.affiliation: Division of Life Sciences, Hallym University, Chunchon 200-702, Korea.
authors.forename: Li Hua
authors.initials: LH
authors.lastname: Jin
authors.forename: Dae Won
authors.initials: DW
authors.lastname: Kim
authors.forename: Won Sik
authors.initials: WS
authors.lastname: Eum
authors.forename: Chang Sik
authors.initials: CS
authors.lastname: Yoon
authors.forename: Sang Ho
authors.initials: SH
authors.lastname: Jang
authors.forename: Hee Soon
authors.initials: HS
authors.lastname: Choi
authors.forename: Soo Hyun
authors.initials: SH
authors.lastname: Choi
authors.forename: Young Hoon
authors.initials: YH
authors.lastname: Kim
authors.forename: So Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Mi Ryoung
authors.initials: MR
authors.lastname: Jung
authors.forename: Tae-Cheon
authors.initials: TC
authors.lastname: Kang
authors.forename: Moo Ho
authors.initials: MH
authors.lastname: Won
authors.forename: Hyeon Yong
authors.initials: HY
authors.lastname: Lee
authors.forename: Jung Hoon
authors.initials: JH
authors.lastname: Kang
authors.forename: Oh-Shin
authors.initials: OS
authors.lastname: Kwon
authors.forename: Sung-Woo
authors.initials: SW
authors.lastname: Cho
authors.forename: Kil Soo
authors.initials: KS
authors.lastname: Lee
authors.forename: Jinseu
authors.initials: J
authors.lastname: Park
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Choi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000235:genetics*; D002374:Catalase / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D002460:Cell Line; D003001:Cloning, Molecular; D003429:Cross Reactions; D004926:Escherichia coli / Q000235:genetics; D015723:Gene Library; D006801:Humans; D008099:Liver / Q000201:enzymology*; D051379:Mice; D008969:Molecular Sequence Data; D051381:Rats; D016415:Sequence Alignment
chemical_list: D000911:Antibodies, Monoclonal; D002374:Catalase