Neuroblastoma oligo-capping cDNA project: toward the understanding of the genesis and biology of neuroblastoma.
Neuroblastoma (NBL) is a common pediatric cancer originated from the neuronal precursor cells of sympathoadrenal lineage. NBLs show a variety of clinical phenotypes from spontaneous regression to malignant progression with acquirement of resistance to therapy. To understand the molecular mechanism of the genesis, progression, and regression of NBL, we need to identify key molecules determining the neuronal development of sympathoadrenal lineage. To this end, we have performed the NBL cDNA project. It includes (1) mass-cloning of the expressed genes from oligo-capping cDNA libraries derived from primary NBLs with different clinical and biological features; (2) mass-identification of differentially expressed genes between favorable and unfavorable subsets; and (3) molecular and functional analyses of the novel genes, which could be useful prognostic indicators. To date, 10,000 cDNA clones in total, approximately 40% of which contained novel sequences, were randomly picked up and DNA sequenced. We have identified approximately 500 differentially expressed genes between favorable and unfavorable subsets of NBL, among which more than 250 were the genes with unknown function.
Ohira
Miki
M
Division of Biochemistry, Chiba Cancer Center Research Institute, 666-2 Nitona, Chuoh-ku, 260-8717 Chiba, Japan.
Morohashi
Aiko
A
Nakamura
Yohko
Y
Isogai
Eriko
E
Furuya
Kazushige
K
Hamano
Shiho
S
Machida
Taiichi
T
Aoyama
Mineyoshi
M
Fukumura
Masayuki
M
Miyazaki
Kou
K
Suzuki
Yutaka
Y
Sugano
Sumio
S
Hirato
Junko
J
Nakagawara
Akira
A
D018076:DNA, Complementary / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D006801:Humans; D008403:Mass Screening; D009447:Neuroblastoma / Q000235:genetics* / Q000473:pathology; D012315:RNA Caps
D018076:DNA, Complementary; D004273:DNA, Neoplasm; D012315:RNA Caps