[Detection of 29 types of fusion gene in leukemia by multiplex RT-PCR].
OBJECTIVE
To analyze the fusion genes derived from 29 types of chromosome structural aberrations in children with leukemia.


METHODS
Bone marrow samples from 191 children with leukemia were analyzed with a novel multiplex nested RT-PCR.


RESULTS
Of the 191 leukemic samples, 86 (45.0%) carried 14 types of fusion genes including SIL/TAL1, MLL/AF1q, E2A/PBX1, MLL/AF6, AML1/ETO, MLL/AF9, TEL/ABL, BCR/ABL, MLL/AF10, dupMLL, MLL/ENL, TEL/AML1, PML/RARalpha and CBFbeta/MYH11. The activation of oncogene HOX11 was detected in 31 cases, with or without other chromosome aberrations in 15 (7.8%) and 16 cases (8.4%), respectively.


CONCLUSIONS
This multiplex nested RT-PCR reaction could screen 29 types of chromosome structural aberrations at the same time. It may be helpful for the detection of minimal residual diseases after chemotherapy and bone marrow transplantation.
Li
Zhi-gang
ZG
Hematological Center, Beijing Children's Hospital, Beijing 100045, China.
Wu
Min-yuan
MY
Zhao
Wei
W
Li
Bin
B
Yang
Jing
J
Zhu
Ping
P
Hu
Ya-mei
YM
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D007938:Leukemia / Q000235:genetics*; D008297:Male; D015514:Oncogene Proteins, Fusion / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D014178:Translocation, Genetic
D015514:Oncogene Proteins, Fusion