title: Choroidal tubercles in disseminated tuberculosis diagnosed by the polymerase chain reaction of aqueous humor. A case report and review of the literature.
abstract: OBJECTIVE
  Disseminated tuberculosis occurs due to the hematogenous spread of pulmonary tuberculosis systemically. Polymerase chain reaction (PCR) of various body fluids has been found to be beneficial in the diagnosis of disseminated tuberculosis, including intraocular tuberculosis. We describe the role of PCR of the aqueous aspirate in a case of disseminated tuberculosis involving the choroid.
  
  
  METHODS
  Single-step PCR fusing primers coding for the IS6110 gene and nested PCR (nPCR) for the detection of the MPB64 gene were performed on the aqueous aspirate in a case of disseminated tuberculosis with miliary involvement of the choroid.
  
  
  RESULTS
  On PCR, the aqueous aspirate showed the Mycobacterium tuberculosis genome, thereby confirming the diagnosis of intraocular tuberculosis.
  
  
  CONCLUSIONS
  PCR is a highly sensitive and specific diagnostic test and PCR of intraocular specimens like the aqueous can be supportive of the diagnosis of miliary tuberculosis of the choroid.
authors.affiliation: Medical and Vision Research Foundations, Chennai, India. drjb@sankaranethralaya.org
authors.forename: Jyotirmay
authors.initials: J
authors.lastname: Biswas
authors.forename: Debraj
authors.initials: D
authors.lastname: Shome
mesh_terms: D001082:Aqueous Humor / Q000382:microbiology*; D015862:Choroid Diseases / Q000382:microbiology* / Q000473:pathology; D005654:Fundus Oculi; D005814:Genes, Viral; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics; D016133:Polymerase Chain Reaction; D014391:Tuberculosis, Miliary / Q000175:diagnosis*