Diallyl disulfide ameliorates gentamicin-induced oxidative stress and nephropathy in rats.
Experimental evidences suggest a role of reactive oxygen species in gentamicin-induced nephropathy in rats. Therefore, we investigated if diallyl disulfide, a garlic-derived compound with antioxidant properties, has a renoprotective effect in this experimental model. Four groups of rats were studied: (1) control, (2) gentamicin treated subcutaneously with gentamicin (70 mg/kg/12 h/4 days), (3) diallyl disulfide treated intragastrically with diallyl disulfide (50 mg/kg/24 h/4 days), and (4) gentamicin + diallyl disulfide treated with gentamicin + diallyl disulfide. Gentamicin induced (a) nephrotoxicity, (b) increase in renal oxidative stress, and (c) decrease in the activity of manganese superoxide dismutase, glutathione peroxidase, and glutathione reductase. Diallyl disulfide ameliorated these changes induced by gentamicin. The mechanism by which diallyl disulfide has a renoprotective effect in gentamicin-induced acute renal failure in rats may be related, at least in part, to the amelioration in the oxidative stress and the preservation in the activity of the antioxidant enzymes in kidney.
Pedraza-Chaverrí
José
J
Department of Biology, Faculty of Chemistry, Universidad Nacional Autónoma de México (UNAM), 04510, México, D.F., Mexico. pedraza@servidor.unam.mx
González-Orozco
Ana E
AE
Maldonado
Perla D
PD
Barrera
Diana
D
Medina-Campos
Omar N
ON
Hernández-Pando
Rogelio
R
D000498:Allyl Compounds / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000009:adverse effects*; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D015415:Biomarkers / Q000032:analysis; D001806:Blood Urea Nitrogen; D004220:Disulfides / Q000494:pharmacology / Q000627:therapeutic use*; D005737:Garlic / Q000737:chemistry; D005839:Gentamicins / Q000009:adverse effects*; D007668:Kidney / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D007674:Kidney Diseases / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
D000498:Allyl Compounds; D000900:Anti-Bacterial Agents; D000975:Antioxidants; D015415:Biomarkers; D004220:Disulfides; D005839:Gentamicins; C028009:diallyl disulfide; D013482:Superoxide Dismutase