title: Trypanosoma cruzi infection induces microfilament depletion in human placenta syncytiotrophoblast.
abstract: Congenital Chagas disease, endemic in Latin America, is associated with premature labour, miscarriage, and placentitis. Metacyclic trypomastigotes adhere to specific receptors on the outer membrane of host cells as a prelude to intracellular invasion, causing calcium ion mobilization, rearrangement of host cell microfilaments, recruitment of lysosomes and parasite internalization. The actin cytoskeleton plays an important role in many cellular processes including the parasite invasion into mammalian cells. In order to observe if placental cytoskeleton is altered in the process of parasite invasion into placental villi, actin microfilaments were studied. Using immunohistochemical techniques, it was observed that the presence of actin in the syncytiotrophoblast was intense throughout the brush border in control placentae belonging to non-chagasic women. But after culture with the trypomastigote, this labelling disappeared, indicating that the parasite induced disassembly of the cortical actin cytoskeleton when the placenta was infected. As a control, placentae from chagasic women were studied, and no actin was found. The same results were obtained by the electron microscope. We confirmed that cortical actin rearrangements may be an early step in the Trypanosoma cruzi invasion mechanism into placental cells, in order to allow lysosomes access to the plasma membrane, and formation of the parasitophorous vacuole. The recruitment of lysosomes occurs directly beneath the invasion site, and this process is required for parasite internalization.
authors.affiliation: IIa. Cátedra de Biología Celular, Histología y Embriología, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, 5016, Córdoba, Argentina. msartori@cmefcm.uncor.edu
authors.forename: M J
authors.initials: MJ
authors.lastname: Sartori
authors.forename: P
authors.initials: P
authors.lastname: Pons
authors.forename: L
authors.initials: L
authors.lastname: Mezzano
authors.forename: S
authors.initials: S
authors.lastname: Lin
authors.forename: S P
authors.initials: SP
authors.lastname: de Fabro
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism / Q000648:ultrastructure*; D000199:Actins / Q000378:metabolism / Q000648:ultrastructure; D000328:Adult; D000818:Animals; D014355:Chagas Disease / Q000150:complications* / Q000378:metabolism / Q000469:parasitology; D002824:Chorionic Villi / Q000378:metabolism / Q000469:parasitology* / Q000648:ultrastructure; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic; D014327:Trophoblasts / Q000378:metabolism / Q000469:parasitology* / Q000648:ultrastructure; D014349:Trypanosoma cruzi / Q000472:pathogenicity / Q000502:physiology*
chemical_list: D000199:Actins