Analysis of polymorphic microsatellites within the bovine and ovine prion protein (PRNP) genes.
Twenty-four microsatellite sites with at least three repeats were found in the bovine prion protein gene (PRNP) and 23 in the ovine PRNP gene. Eight microsatellite sites were polymorphic in cattle and six in sheep with up to 10 alleles per site. In many cases allelic DNA fragments had variants in microsatellite sites and in flanking regions. Distances between microsatellite sites in eight genes from cattle and sheep occurred on average every 0.9 kb. The numerous polymorphic microsatellite sites will improve analysis of phylogenetic origin of different PRNP alleles and trait association studies for bovine spongiform encephalopathy (BSE) and scrapie.
Geldermann
H
H
Department of Animal Breeding and Biotechnology, University of Hohenheim, Stuttgart, Germany. tzunihoh@uni-hohenheim.de
Preuss
S
S
Eckert
J
J
Han
Y
Y
Ollesch
K
K
D000682:Amyloid / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping; D017931:DNA Primers; D004586:Electrophoresis; D005787:Gene Frequency; D018895:Microsatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D011328:Prions; D011498:Protein Precursors / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012756:Sheep / Q000235:genetics*
D000682:Amyloid; D017931:DNA Primers; D011328:Prions; D011498:Protein Precursors