Whole-field fluorescence microscope with digital micromirror device: imaging of biological samples.
We have developed a whole-field fluorescence microscope equipped with a Digital Micromirror Device to acquire optically sectioned images by using the fringe-projection technique and the phase-shift method. This system allows free control of optical sectioning strength through computer-controlled alteration of the fringe period projected onto a sample. We have employed this system to image viable cells expressing fluorescent proteins and discussed its biological applications.
Fukano
Takashi
T
Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Institute of Brain Science, The Institute of Physical and Chemical Research, 2-1 Hirosawa, Wako-city, Saitama 351-0198, Japan. tenchan@brain.riken.go.jp
Miyawaki
Atsushi
A
D002118:Calcium / Q000378:metabolism*; D002135:Calcium-Binding Proteins; D029721:Drosophila Proteins; D004867:Equipment Design; D005240:Feasibility Studies; D006367:HeLa Cells / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D007368:Interferometry / Q000295:instrumentation / Q000379:methods; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D008904:Miniaturization; D055095:Optics and Photonics / Q000295:instrumentation*; D014054:Tomography / Q000295:instrumentation / Q000379:methods
D002135:Calcium-Binding Proteins; D029721:Drosophila Proteins; C473067:cameleon 2.1 protein, Drosophila; D002118:Calcium