title: Detection of Cyclospora cayetanensis in travellers returning from the tropics and subtropics using microscopy and real-time PCR.
abstract: We examined 100 stool specimens of returning travellers with diarrhoea for the presence of Cyclospora cayetanensis using fluorescence microscopy and real-time PCR. C. cayetanensis was found in four cases with microscopy and PCR. One additional sample was positive only by PCR, and could be confirmed by microscopic examination of several additional slides. C. cayetanensis was the most frequent parasitic cause of diarrhoea after Giardia duodenalis.
authors.affiliation: Department of Parasitology, Leiden University Medical Centre, Leiden, The Netherlands. j.j.verweij@LUMC.nl
authors.forename: Jaco J
authors.initials: JJ
authors.lastname: Verweij
authors.forename: Daphne
authors.initials: D
authors.lastname: Laeijendecker
authors.forename: Eric A T
authors.initials: EA
authors.lastname: Brienen
authors.forename: Lisette
authors.initials: L
authors.lastname: van Lieshout
authors.forename: Anton M
authors.initials: AM
authors.lastname: Polderman
mesh_terms: D000818:Animals; D021744:Cyclospora / Q000235:genetics / Q000302:isolation & purification*; D021866:Cyclosporiasis / Q000175:diagnosis*; D003967:Diarrhea / Q000469:parasitology*; D005243:Feces / Q000469:parasitology; D006801:Humans; D008856:Microscopy, Fluorescence; D010270:Parasite Egg Count; D016133:Polymerase Chain Reaction; D016053:RNA, Protozoan / Q000032:analysis; D012335:RNA, Ribosomal / Q000032:analysis; D014195:Travel; D014329:Tropical Climate
chemical_list: D016053:RNA, Protozoan; D012335:RNA, Ribosomal