Evaluation of formalin-acetone sedimentation in the concentration of stool for intestinal parasites.
Formalin-acetone sedimentation was compared with the formalin-ether method for the concentration of stool for intestinal parasites. Of 80 stool specimens, 45 (56.25%) were positive for parasites by the formalin-acetone method. The figures for the two methods were formalin-ether 35 (43.75%) and for the direct lacto-phenol cotton blue wet mount method 17 (21.25%). There was no statistically significant difference in the parasite recovery rate between the two methods. Acetone is more stable, safer, and a cheaper fat solvent and promises to be a useful alternative to ether.
Parija
S C
SC
Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research, Pondicherry, India. parijasc@vsnl.com
Bhattacharya
S
S
Padhan
P
P
Shivaprakash
M R
MR
D000096:Acetone; D000818:Animals; D004986:Ether; D056890:Eukaryota / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology*; D005404:Fixatives; D005557:Formaldehyde; D006376:Helminths / Q000302:isolation & purification*; D006801:Humans; D007411:Intestinal Diseases, Parasitic / Q000175:diagnosis* / Q000469:parasitology; D010270:Parasite Egg Count / Q000379:methods*; D012997:Solvents
D005404:Fixatives; D012997:Solvents; D004986:Ether; D000096:Acetone; D005557:Formaldehyde