Use of chitosan membrane from the carapace of the soldier crab Mictyris brevidactylus for biosensor construction.
Glucose oxidase (EC 1.3.4.3) was immobilized on chitosan membrane (<0.1 mm in thickness) prepared from the carapace of the soldier crab Mictyris brevidactylus. A glucose electrode was constructed by covering a platinum electrode (2.0 mm in diameter) with the enzyme membrane. The enzyme electrode sensed glucose amperometrically (1.0 micro A/mM, with linear range up to 0.5 mM, r = 0.999) when positively imposed with 0.6 V against an Ag/AgCl reference electrode. The glucose biosensor was sensitive (<0.1 micro M, S/N > 3), reproducible (CV for 55 micro M glucose <3%, n = 5), reagentless, and durable for months.
Hsieh
Bo-Chuan
BC
Department of Bio-Industrial Mechatronics Engineering, College of Agriculture, National Taiwan University, 1, Roosevelt Road, Sec. 4, Taipei, Taiwan 106, Republic of China.
Cheng
Tzong-Jih
TJ
Wang
Tzu-Yu
TY
Chen
Richie L C
RL
D000818:Animals; D001672:Biocompatible Materials; D001704:Biopolymers / Q000302:isolation & purification; D015374:Biosensing Techniques / Q000295:instrumentation*; D003386:Brachyura / Q000737:chemistry; D002686:Chitin / Q000031:analogs & derivatives* / Q000302:isolation & purification; D048271:Chitosan; D004566:Electrodes; D004800:Enzymes, Immobilized; D004867:Equipment Design; D005069:Evaluation Studies as Topic; D005947:Glucose / Q000032:analysis*; D008422:Materials Testing; D008567:Membranes, Artificial
D001672:Biocompatible Materials; D001704:Biopolymers; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D002686:Chitin; D048271:Chitosan; D005947:Glucose