Prolonged procoagulant activity on overstretch-injured coronary arteries in pigs.
This study was designed to assess the time course and nature of the vascular procoagulant response after 1.5-fold balloon overstretch injury of the coronary arteries in pigs. Arteries were excised for chromogenic assay of bound factor (F)Xa and thrombin at 24 h, 3 days, 1 week, or 2 weeks after injury. FXa at the site of injury remained elevated for 1 week (4.9 +/- 5.9 microg cm(-2), n = 10), compared with non-injured control arteries (0.4 +/- 0.2 microg cm(-2), n = 18, P = 0.00025), while thrombin was increased only at 24 h. Tissue factor protein was abundant in non-injured coronaries (10 +/- 6 ng microg(-1) total protein, n = 9) and levels were unchanged by injury (13 +/- 11 ng microg(-1), n = 6) or 24-h administration of tissue factor pathway inhibitor (16 +/- 6 ng microg(-1), n = 6). Persistent tissue factor-mediated procoagulant activity may explain the need for prolonged anticoagulation to attenuate neointimal formation after balloon-induced coronary injury.
Abendschein
D R
DR
Center for Cardiovascular Research, Washington University School of Medicine, St. Louis, Missouri 63110, USA. dabendsc@imgate.wustl.edu
Yang
L Y
LY
Chun
J
J
Cho
D
D
Scherrer
D
D
St Pierre
J
J
D015906:Angioplasty, Balloon, Coronary / Q000009:adverse effects*; D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects / Q000502:physiology*; D023903:Coronary Restenosis / Q000188:drug therapy; D003331:Coronary Vessels / Q000187:drug effects / Q000293:injuries* / Q000503:physiopathology*; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology; D015951:Factor Xa / Q000032:analysis; D005343:Fibrinolytic Agents / Q000494:pharmacology / Q000627:therapeutic use; D007700:Kinetics; D008074:Lipoproteins / Q000494:pharmacology / Q000627:therapeutic use; D008297:Male; D023421:Models, Animal; D013552:Swine; D013917:Thrombin / Q000032:analysis; D013925:Thromboplastin / Q000032:analysis
D005343:Fibrinolytic Agents; D008074:Lipoproteins; C051928:lipoprotein-associated coagulation inhibitor; D013925:Thromboplastin; D013917:Thrombin; D015951:Factor Xa