Cytotoxicity evaluation of single component dentin bonding agents.
This study evaluated the cytotoxicity of four single component dentin bonding agents: Syntac Single Component, Prime & Bond 2.1, Single Bond and One Up Bond F. The test materials were applied on dentin discs of dentin barrier models in the same way as in the clinical procedures recommended by each manufacturer. Cell viability of L 929 after exposure with the bonding agents was determined by MTT assay. The results revealed that cell survival of the first three bonding agents was 60%, while the fourth was an impressive 93%. This study showed that a total-etching bonding system is more cytotoxic than a self-etching bonding system.
Vajrabhaya
La-ongthong
LO
Dept of Operative Dentistry, Faculty of Dentistry, Mahidol University, Bangkok, Thailand. dtlvj@mahidol.ac.th
Pasasuk
Apaporn
A
Harnirattisai
Choltacha
C
D000096:Acetone / Q000737:chemistry / Q000633:toxicity; D000134:Acid Etching, Dental / Q000009:adverse effects*; D000328:Adult; D000818:Animals; D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry / Q000633:toxicity; D003782:Dental Pulp / Q000187:drug effects*; D017220:Dentin-Bonding Agents / Q000737:chemistry / Q000633:toxicity*; D015194:Diffusion Chambers, Culture; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D007739:L Cells; D008689:Methacrylates / Q000737:chemistry / Q000633:toxicity; D051379:Mice; D011109:Polymethacrylic Acids / Q000737:chemistry / Q000633:toxicity; D019279:Resin Cements / Q000737:chemistry / Q000633:toxicity*; D016369:Smear Layer
D017220:Dentin-Bonding Agents; D008689:Methacrylates; C446260:One-Up-Bond F; D011109:Polymethacrylic Acids; D019279:Resin Cements; C078484:Syntac dentine adhesive; C444444:prime and bond 2.1; C115612:single bond; D000096:Acetone; D017438:Bisphenol A-Glycidyl Methacrylate