title: High transfection efficiency of poly(4-vinylimidazole) as a new gene carrier.
abstract: Poly(4-vinylimidazole) (P4V) was obtained by free radical polymerization of 4-vinylimidazole (4V) prepared by decarboxylation of urocanic acid. P4V formed a complex with DNA that exhibited higher transfection effiency on Hela cells than polyethylenimine (PEI), through the proton sponge mechanism of the imidazole groups in the side chain of the P4V, and low cell toxicity.
authors.affiliation: School of Agricultural Biotechnology, Seoul National University, Suwon 441-744, Korea.
authors.forename: Jong-Eun
authors.initials: JE
authors.lastname: Ihm
authors.forename: Ki-Ok
authors.initials: KO
authors.lastname: Han
authors.forename: In-Kwon
authors.initials: IK
authors.lastname: Han
authors.forename: Kwang-Duk
authors.initials: KD
authors.lastname: Ahn
authors.forename: Dong-Keun
authors.initials: DK
authors.lastname: Han
authors.forename: Chong-Su
authors.initials: CS
authors.lastname: Cho
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D015316:Genetic Therapy / Q000379:methods; D006367:HeLa Cells; D006801:Humans; D007093:Imidazoles / Q000138:chemical synthesis* / Q000737:chemistry* / Q000494:pharmacology; D051379:Mice; D008956:Models, Chemical; D008970:Molecular Weight; D010316:Particle Size; D011145:Polyvinyls / Q000138:chemical synthesis* / Q000737:chemistry* / Q000494:pharmacology; D014162:Transfection / Q000379:methods*
chemical_list: D004337:Drug Carriers; D007093:Imidazoles; D011145:Polyvinyls; C482913:poly(4-vinylimidazole); D004247:DNA