Engineering tolerance and accumulation of lead and cadmium in transgenic plants.
We have studied the utility of the yeast protein YCF1, which detoxifies cadmium by transporting it into vacuoles, for the remediation of lead and cadmium contamination. We found that the yeast YCF1-deletion mutant DTY167 was hypersensitive to Pb(II) as compared with wild-type yeast. DTY167 cells overexpressing YCF1 were more resistant to Pb(II) and Cd(II) than were wild-type cells, and accumulated more lead and cadmium. Analysis of transgenic Arabidopsis thaliana plants overexpressing YCF1 showed that YCF1 is functionally active and that the plants have enhanced tolerance of Pb(II) and Cd(II) and accumulated greater amounts of these metals. These results suggest that transgenic plants expressing YCF1 may be useful for phytoremediation of lead and cadmium.
Song
Won-Yong
WY
National Research Laboratory for Phytoremediation, Division of Molecular Life Sciences, Pohang, 790-784, Korea.
Sohn
Eun Ju
EJ
Martinoia
Enrico
E
Lee
Yong Jik
YJ
Yang
Young-Yell
YY
Jasinski
Michal
M
Forestier
Cyrille
C
Hwang
Inwhan
I
Lee
Youngsook
Y
D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002104:Cadmium / Q000493:pharmacokinetics*; D003001:Cloning, Molecular; D004361:Drug Tolerance / Q000502:physiology*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D005818:Genetic Engineering / Q000379:methods*; D024861:Genetic Enhancement / Q000379:methods; D007220:Industrial Waste / Q000517:prevention & control; D007854:Lead / Q000493:pharmacokinetics*; D030821:Plants, Genetically Modified / Q000378:metabolism; D012037:Refuse Disposal / Q000379:methods; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D012989:Soil Pollutants / Q000493:pharmacokinetics
D018528:ATP-Binding Cassette Transporters; D007220:Industrial Waste; D029701:Saccharomyces cerevisiae Proteins; D012989:Soil Pollutants; C089179:YCF1 protein, S cerevisiae; D002104:Cadmium; D007854:Lead