Central expression of c-Fos in neonatal male and female prairie voles in response to treatment with oxytocin.
Early postnatal exposure to both exogenous and endogenous oxytocin (OT) can have long-term effects on behavior and physiology, although the mechanisms of these effects are not known. c-Fos expression was used to investigate the immediate neural effects of neonatal manipulations of OT in male and female prairie voles. On the day of birth prairie vole pups received an intraperitoneal injection of OT, a selective OT antagonist (OTA), or saline (vehicle control), while an additional group was handled but not injected. One hour after treatment brains were collected and fixed via spinning immersion and immunocytochemistry was then used to label for c-Fos immunoreactivity (IR). There were significant differences between males and females. Handled only females displayed significantly higher levels of c-Fos IR in the mediodorsal thalamic nucleus (MD) than males while handled males had higher c-Fos IR in the paraventricular nucleus of the hypothalamus than females. Exogenous OT stimulated neuronal activity in the supraoptic nucleus (SON) in males, while treatment with OTA increased Fos IR in the SON and was associated with reduced Fos IR in the MD in females. The results indicate that neuronal activity and responses to OT are sexually dimorphic in newborn prairie voles. In females changes in Fos expression were stimulated by treatment with OTA, suggesting that endogenous OT affects cellular activity while males responded to exogenous OT.
Cushing
Bruce S
BS
Department of Biology, University of Maryland, College Park, MD 20742, USA. bcushing@psych.uic.edu
Yamamoto
Yukiyo
Y
Hoffman
Gloria E
GE
Carter
C Sue
CS
D000818:Animals; D000831:Animals, Newborn; D003411:Arvicolinae; D001921:Brain / Q000187:drug effects* / Q000502:physiology*; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D010121:Oxytocin / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D010812:Physical Stimulation; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis* / Q000187:drug effects*; D012727:Sex Characteristics
D016760:Proto-Oncogene Proteins c-fos; D010121:Oxytocin