title: Osmotic stimuli increase brain-derived neurotrophic factor mRNA level in the rat subfornical organ.
abstract: The effects of water deprivation and chronic salt loading on the expression of the brain-derived neurotrophic factor (BDNF) gene were examined in the rat subfornical organ (SFO), using immunohistochemistry for BDNF and in situ hybridization histochemistry. Increased BDNF-like immunoreactivity was observed in the SFO after water deprivation for 4 days. Water deprivation for 24 h and 2 and 4 days and salt loading for 7 days caused a significant increase in the BDNF gene transcripts in the SFO, compared with euhydrated rats. These results suggest that BDNF in the SFO may be involved in the regulatory mechanisms of body fluid balance.
authors.affiliation: Department of Physiology, School of Medicine, University of Occupational and Environmental Health, 807-8555 Kitakyushu, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Saito
authors.forename: Yumi
authors.initials: Y
authors.lastname: Ozaki
authors.forename: Hideo
authors.initials: H
authors.lastname: Ohnishi
authors.forename: Toshitaka
authors.initials: T
authors.lastname: Nakamura
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Ueta
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D009997:Osmotic Pressure; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D017673:Sodium Chloride, Dietary / Q000008:administration & dosage; D013356:Subfornical Organ / Q000378:metabolism*; D014868:Water Deprivation
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D012333:RNA, Messenger; D017673:Sodium Chloride, Dietary