Optimization of cell growth and poly(glutamic acid) production in batch fermentation by Bacillus subtilis.
Poly(glutamic acid) was produced maximally by Bacillus subtilis in batch fermentations at pH 7 and using glycerol at 20 g l(-1) in a glutamic acid/citric acid medium. Poly(glutamic acid) reached 23 g l(-1) after 30 h.
Richard
Andrew
A
Department of Chemical and Biochemical Engineering, The University of Western Ontario, London, Ontario, Canada, N6A 5B9.
Margaritis
Argyrios
A
D001412:Bacillus subtilis / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism*; D019149:Bioreactors; D002455:Cell Division / Q000502:physiology; D019343:Citric Acid / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D011099:Polyglutamic Acid / Q000096:biosynthesis*; D011786:Quality Control
D003470:Culture Media; D011099:Polyglutamic Acid; D019343:Citric Acid; D018698:Glutamic Acid; D005990:Glycerol