title: [Inhibition of duck hepatitis B virus DNA replication by antisense phosphorothioate oligodeoxynucleotides in vitro and in vivo].
abstract: BACKGROUND
  To determine the feasibility of inhibition of duck hepatitis B virus (DHBV) DNA replication by antisense phosphorothioate oligodeoxynucleotides corresponding to DHBV transcription region.
  
  
  METHODS
  The authors designed three antisense phosphorothioate oligodeoxynucleotides which correspond to DHBV PreS1,PreS2 and S antigen gene promotors respectively. The DNA replication level was detected with Southern blot method and cpm calculation.
  
  
  RESULTS
  Primary duck hepatocyte culture was treated with 1.5 micromol/L antisense oligodeoxynucleotides in vitro, all the antisense fragments caused a firm inhibition of viral DNA replication and the inhibition rates were 61.5%, 69.3% and 62.4%, respectively. In vivo, the animals were treated with 10 microgram/g PreS1 antigen gene promotor antisense oligodeoxynucleotides per day for 6 days and a very strong inhibition rate of 87.9% was obtained.
  
  
  CONCLUSIONS
  The results demonstrated the potential clinical application of antisense phosphorothioate oligodeoxynucleotides in clinics.
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical University, Beijing 100050, China.
authors.forename: Biao
authors.initials: B
authors.lastname: Dong
authors.forename: Xing-wu
authors.initials: XW
authors.lastname: Shao
authors.forename: Pei-zhen
authors.initials: PZ
authors.lastname: Tao
mesh_terms: D000818:Animals; D004261:DNA Replication / Q000187:drug effects*; D004279:DNA, Viral / Q000187:drug effects; D004372:Ducks; D018347:Hepadnaviridae Infections / Q000821:virology*; D006514:Hepatitis B Surface Antigens / Q000097:blood; D015611:Hepatitis B Virus, Duck / Q000235:genetics* / Q000502:physiology; D006524:Hepatitis, Viral, Animal / Q000821:virology*; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D011498:Protein Precursors / Q000097:blood; D014779:Virus Replication / Q000187:drug effects
chemical_list: D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D020319:Oligodeoxyribonucleotides, Antisense; D011498:Protein Precursors; C050806:presurface protein 1, hepatitis B surface antigen; C050807:presurface protein 2, hepatitis B surface antigen