title: Estimation of the optimal electrophoretic temperature of DNA single-strand conformation polymorphism by DNA base composition.
abstract: To explore the relation between DNA base composition and the optimal single-strand conformation polymorphism (SSCP) electrophoretic temperature (T(s)), we analyzed DNA base composition and T(s) of 24 DNA fragments from different genes and found that T(s) was positively correlative with the ratio of base C/base A. T(s) could be estimated by the formula T(s) = [80 x C/(A+1)]/[2.71 + [C/(A+1)]]. T(s) could be increased dramatically by the complementary sequences in both 5'-and 3'-ends of a DNA single-strand.
authors.affiliation: Laboratory of Pediatrics, Medical Scientific Research Center, Guangxi Medical University, China. liwei60@yahoo.com
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Junlin
authors.initials: J
authors.lastname: Liang
authors.forename: Chensheng
authors.initials: C
authors.lastname: Li
authors.forename: Haitian
authors.initials: H
authors.lastname: Zhang
authors.forename: Zongjiang
authors.initials: Z
authors.lastname: Tang
authors.forename: Lisheng
authors.initials: L
authors.lastname: Chen
authors.forename: Qi
authors.initials: Q
authors.lastname: Jin
authors.forename: Weizhong
authors.initials: W
authors.lastname: Tang
mesh_terms: D001482:Base Composition; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D018807:Polymorphism, Single-Stranded Conformational; D013696:Temperature
chemical_list: D017931:DNA Primers; D004247:DNA