title: [Effect of severe thoracic trauma combined with endotoxin challenge on phagocytosis function of alveolar macrophages and pulmonary interstitial macrophages].
abstract: OBJECTIVE
  To observe the difference of phagocytosis between alveolar macrophages and pulmonary interstitial macrophages, and to investigate their responses to severe thoracic trauma with or without lipopolysaccharide (LPS) challenge.
  
  
  METHODS
  A rat model of severe thoracic trauma was reproduced by thoracic impact machine. The alveolar macrophages and interstitial macrophages were isolated before injury and at 2, 4, 8, 16, 24 hours after injury respectively. The dynamic changes of these macrophage phagocytosis were tested by malachite green colorimetry.
  
  
  RESULTS
  Macrophage phagocytosis function was increased during the early stage after trauma (2 and 4 hours) and then decreased. The phagocytosis function of alveolar macrophages was stronger than that of interstitial macrophages in all time points before and after trauma. After challenge with LPS, no further significant effect on the alveolar macrophages was found, while LPS challenge could stimulate the phagocytosis of interstitial macrophages.
  
  
  CONCLUSIONS
  Alveolar macrophages and pulmonary interstitial macrophages are functional heterogeneous, and their response to trauma and combined with endotoxin challenge are different. The results indicate that the two subgroups of macrophages play different roles in immune function disorder after trauma.
authors.affiliation: Center of Thoracic Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Yao-guang
authors.initials: YG
authors.lastname: Jiang
authors.forename: Zhi-jian
authors.initials: ZJ
authors.lastname: Xie
authors.forename: Cheng-xiang
authors.initials: CX
authors.lastname: Hu
authors.forename: Lei
authors.initials: L
authors.lastname: Li
mesh_terms: D000818:Animals; D004731:Endotoxins; D008168:Lung / Q000166:cytology; D016676:Macrophages, Alveolar / Q000502:physiology*; D010587:Phagocytosis; D051381:Rats; D017208:Rats, Wistar; D017154:Stromal Cells / Q000502:physiology*; D013898:Thoracic Injuries / Q000382:microbiology*
chemical_list: D004731:Endotoxins