title: A case of pulmonary aspergilloma molecular biological identification and typing of the isolates from antemortem sputa and autopsy fungus ball.
abstract: Filamentous fungi were isolated from antemortem sputum and an autopsy fungus ball of the lung in a case of aspergilloma. Both of the isolates were analyzed for the sequences of species or strain-specific nuclear ribosomal DNA (partial 28S and ITS1 regions), and were identified as Aspergillus fumigatus. The molecular biological technique saved time and is thought to be a powerful tool in the accurate diagnosis of pulmonary fungal infection to assure effective treatment.
authors.affiliation: First Department of Internal Medicine, Tokyo Woman's Medical University, Tokyo, Japan.
authors.forename: C
authors.initials: C
authors.lastname: Sugita
authors.forename: K
authors.initials: K
authors.lastname: Makimura
authors.forename: A
authors.initials: A
authors.lastname: Murakami
authors.forename: Y
authors.initials: Y
authors.lastname: Murai
authors.forename: H
authors.initials: H
authors.lastname: Yamaguchi
authors.forename: A
authors.initials: A
authors.lastname: Nagai
mesh_terms: D001228:Aspergillosis / Q000382:microbiology* / Q000473:pathology; D001232:Aspergillus fumigatus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001344:Autopsy; D001483:Base Sequence; D021903:DNA, Ribosomal Spacer / Q000032:analysis*; D006801:Humans; D008168:Lung / Q000382:microbiology / Q000473:pathology; D008172:Lung Diseases, Fungal / Q000382:microbiology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D012339:RNA, Ribosomal, 28S / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013183:Sputum / Q000382:microbiology
chemical_list: D021903:DNA, Ribosomal Spacer; D012339:RNA, Ribosomal, 28S