Effects of the mycotoxin ochratoxin A and some of its metabolites on human kidney cell lines.
The modulations of complement-regulating surface proteins on a human embryonic and a renal carcinoma cell line are described regarding the effects of ochratoxin A and some of its metabolites on the surface markers CD46, CD55 and CD59. Membrane integrity, cell proliferation and metabolic activity were reduced to different extents, depending on the kind of mycotoxin and the dosage, which was ranging from 10 to 1000 ng/ml. The number of cells carrying surface markers was suppressed significantly at 1000 ng/ml, in some cases even at 100 ng/ml, whereas the intensity of receptor expression on the positive cells was found to be stimulated. The fraction RE2 (OTC) isolated from an OTA-containing crude toxin surpassed the effects of all other ochratoxin metabolites. Apart from well-known cytotoxic and genotoxic effects modulation of cell surface marker expression by low concentrations of OTA and OTC deserves more attention with regard to its immuno-pathogenic importance. Furthermore, occurrence and impact of the mycotoxin OTC should be studied more into detail.
Müller
G
G
Federal Institute for Health Protection of Consumers and Veterinary Medicine, Division 4, Naumburger Str. 96a, D-07743, Jena, Germany.
Burkert
B
B
Rosner
H
H
Köhler
H
H
D015703:Antigens, CD / Q000096:biosynthesis; D000954:Antigens, Surface / Q000096:biosynthesis; D018958:CD55 Antigens / Q000096:biosynthesis; D018957:CD59 Antigens / Q000096:biosynthesis; D002273:Carcinogens / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000648:ultrastructure; D004396:Coloring Agents; D006801:Humans; D007668:Kidney / Q000166:cytology* / Q000187:drug effects; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D009793:Ochratoxins / Q000633:toxicity*; D010084:Oxidation-Reduction; D011419:Propidium; D013778:Tetrazolium Salts; D013844:Thiazoles
D015703:Antigens, CD; D000954:Antigens, Surface; C497857:CD46 protein, human; D018958:CD55 Antigens; D018957:CD59 Antigens; D002273:Carcinogens; D004396:Coloring Agents; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins; D009793:Ochratoxins; D013778:Tetrazolium Salts; D013844:Thiazoles; C025589:ochratoxin A; D011419:Propidium; C022616:thiazolyl blue