Coexpression of matrilysin and laminin-5 gamma2 chain may contribute to tumor cell migration in colorectal carcinomas.
We attempted to examine the correlation between matrilysin and laminin-5 gamma2 chain expression with reference to the number of dedifferentiation units along the entire invasive front (tumor budding). Immunostaining for hMMP-7 and laminin-5 gamma2 chain was performed in 50 T1 colorectal carcinomas, and immunoreactivity was evaluated at the invasive front of the tumor. On hematoxylin-eosin sections, the number of tumor budding was counted. The localization of matrilysin tended to be widespread compared with that of laminin-5 gamma2 chain. Matrilysin and laminin-5 gamma2 chain expression were positive in 28 (56%) and 15 (30%) tumors respectively. There was a significant correlation between matrilysin and laminin-5 gamma2 chain expression (P = 0.02). Matrilysin(+)/laminin-5 gamma2 chain(+) tumors had a significantly greater amount of tumor budding than matrilysin(-)/laminin-5 gamma2 chain(-) tumors (P = 0.003) or matrilysin(+)/laminin-5 gamma2 chain(-) tumors (P = 0.03). In conclusions, coexpression of matrilysin and laminin-5 gamma2 chain may contribute to tumor cell migration in colorectal carcinomas.
Masaki
Tadahiko
T
First Department of Surgery, Kyorin University, 6-20-2, Shinkawa, Mitaka city, Tokyo 181-8611, Japan.
Sugiyama
Masanori
M
Matsuoka
Hiroyoshi
H
Abe
Nobutsugu
N
Izumisato
Yumi
Y
Sakamoto
Atsuhiko
A
Atomi
Yutaka
Y
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D002465:Cell Movement / Q000502:physiology*; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007797:Laminin / Q000096:biosynthesis*; D008297:Male; D020783:Matrix Metalloproteinase 7 / Q000096:biosynthesis*; D020782:Matrix Metalloproteinases / Q000378:metabolism; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D014407:Tumor Cells, Cultured; D000097794:Kalinin
D015815:Cell Adhesion Molecules; D007797:Laminin; D020783:Matrix Metalloproteinase 7; D020782:Matrix Metalloproteinases; D000097794:Kalinin; C400567:LAMC2 protein, human