title: Paragonimus westermani: molecular cloning, expression, and characterization of a recombinant yolk ferritin.
abstract: Ferritin is an intracellular protein involved in iron metabolism. A cDNA PwYF-1 cloned from the adult Paragonimus westermani cDNA library encoded a putative polypeptide of 216 amino acids homologous with ferritins of vertebrates and invertebrates. Febinding motifs identified in PwYF-1 polypeptide were conserved and predicted to form a ferroxidase center. PwYF-1 polypeptide contained an extended peptide of 45 amino acids at its C-terminus. Recombinant PwYF-1 protein, expressed and purified from Escherichia coli, showed iron-uptake ability and ferroxidase activity. Ferroxidase activity of recombinant PwYF-1 protein was reactivated by secondary addition of apotransferrin to assay mixture. Mouse immune serum raised against the recombinant PwYF-1 protein recognized specifically 24 kDa protein from adult P. westermani lysate. PwYF-1 protein was localized to vitelline follicles and the eggs of P. westermani. Collectively, PwYF-1 protein was identified as a P. westermani yolk ferritin.
authors.affiliation: Department of Parasitology, Seoul National University College of Medicine, Seoul 110-799, South Korea.
authors.forename: Tae Yun
authors.initials: TY
authors.lastname: Kim
authors.forename: Il Jung
authors.initials: IJ
authors.lastname: Joo
authors.forename: Shin-Yong
authors.initials: SY
authors.lastname: Kang
authors.forename: Seung-Yull
authors.initials: SY
authors.lastname: Cho
authors.forename: Sung-Jong
authors.initials: SJ
authors.lastname: Hong
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003400:Astacoidea; D001483:Base Sequence; D002570:Ceruloplasmin / Q000378:metabolism; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D018509:DNA, Helminth / Q000737:chemistry; D004285:Dogs; D005293:Ferritins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D005786:Gene Expression Regulation; D015151:Immunoblotting; D007150:Immunohistochemistry; D007501:Iron / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010238:Paragonimus / Q000235:genetics* / Q000378:metabolism; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment
chemical_list: D018076:DNA, Complementary; D018509:DNA, Helminth; D011994:Recombinant Proteins; D005293:Ferritins; D007501:Iron; D002570:Ceruloplasmin