Probing the structure and function of the Escherichia coli DNA alkylation repair AlkB protein through chemical cross-linking.
Engineering chemical cross-linking groups at the protein/DNA interface provide a powerful method for probing the putative active site and a damage searching mechanism of the Escherichia coli alkylation DNA repair protein AlkB.
Mishina
Yukiko
Y
Department of Chemistry, The University of Chicago, 5735 South Ellis Avenue, Chicago, Illinois 60637, USA.
He
Chuan
C
D000478:Alkylation; D001483:Base Sequence; D001665:Binding Sites; D003432:Cross-Linking Reagents / Q000737:chemistry; D004260:DNA Repair; D004269:DNA, Bacterial / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000378:metabolism; D004220:Disulfides / Q000737:chemistry; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology*; D006899:Mixed Function Oxygenases / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology*; D013329:Structure-Activity Relationship; D015032:Zinc / Q000737:chemistry
D003432:Cross-Linking Reagents; D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D004220:Disulfides; D029968:Escherichia coli Proteins; D006899:Mixed Function Oxygenases; C466427:AlkB protein, E coli; D015032:Zinc