Changes in zinc, copper and metallothionein contents during oocyte growth and early development of the teleost Danio rerio (zebrafish).
In the present report, we investigated zinc, copper and metallothionein (MT) contents in zebrafish oocytes and embryos. Our results demonstrate that the metal content increases during oocytes maturation. Zinc increases from 30 ng/oocyte (stage-1 oocytes) to 100 ng/oocyte (stage-3 oocytes); copper varied from 1 ng/oocyte (stage-1 oocytes) to 3.5 ng/oocyte (stage-3 oocytes). During embryogenesis, zinc and copper contents dramatically increase after fertilisation around the 512-cells stage, then slowly decrease until the mid-gastrula stage. During oocyte growth, the changes in the MT level are proportional to metal content, whereas during embryogenesis the pattern of MT accumulation does not parallel that of the two metals. Indeed, the maternal pool of MT decreases steadily during the early stages of the development until the gastrula stage. We have examined the effect of cadmium on the expression of MT during zebrafish development. After cadmium exposure, MT content increases in embryos at the blastula stage, whereas no induction occurs in embryos at the gastrula stage. However, pre-treatment of embryos at the gastrula stage with 5-aza-2'-deoxycytidine induces MT synthesis following exposure to cadmium. These observations show that changes in metal levels are not correlated to MT content in the embryo, whereas DNA methylation is one of the factors regulating MT expression.
Riggio
Marilisa
M
Department of Evolutionary and Comparative Biology, University of Naples Federico II, via Mezzocannone 8, I-80134 Naples, Italy.
Filosa
Silvana
S
Parisi
Elio
E
Scudiero
Rosaria
R
D000818:Animals; D002104:Cadmium / Q000494:pharmacology; D003300:Copper / Q000032:analysis*; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000378:metabolism; D047108:Embryonic Development; D005260:Female; D008297:Male; D008668:Metallothionein / Q000032:analysis* / Q000096:biosynthesis; D009865:Oocytes / Q000254:growth & development* / Q000378:metabolism*; D015027:Zebrafish / Q000196:embryology* / Q000254:growth & development / Q000378:metabolism*; D015032:Zinc / Q000032:analysis*
D002104:Cadmium; D003300:Copper; D008668:Metallothionein; D015032:Zinc