title: Effect of estradiol on chemokine receptor CXCR2 expression in rats: implications for atherosclerosis.
abstract: OBJECTIVE
  To study the effect of 17beta-estradiol on expression of chemokine receptor CXCR2 in monocytes in vivo.
  
  
  METHODS
  Expressions of chemokine receptor CXCR2 mRNA and protein were measured by RT-PCR and flow cytometry, respectively.
  
  
  RESULTS
  In both ovary-intact and ovariectomized (OVX) rats, CXCR2 protein and mRNA expression were significantly increased in rats fed with a high-cholesterol diet for 6 weeks. The cholesterol-induced increases in CXCR2 protein and mRNA expression were significantly attenuated in OVX rats injected with estradiol-17beta (17beta-E2) (5 and 20 microg x kg(-1) x d(-1)). In normal diet rats, CXCR2 protein and mRNA expression were increased in OVX rats compared with ovary-intact rats, and this increase was prevented by 17beta-E2.
  
  
  CONCLUSIONS
  Both basal and hypercholesterolemia-induced increases in chemokine receptor CXCR2 are modulated by physiological concentrations of estradiol.
authors.affiliation: Department of Cardiology, General Hospital of Ji'nan Military Area, Ji'nan 250031, China. leizhubin88@email.com.cn
authors.forename: Zhu-Bin
authors.initials: ZB
authors.lastname: Lei
authors.forename: Xu-Jie
authors.initials: XJ
authors.lastname: Fu
authors.forename: Zhao-Tong
authors.initials: ZT
authors.lastname: Lu
authors.forename: Bao-Cheng
authors.initials: BC
authors.lastname: Wang
authors.forename: Xian-Liang
authors.initials: XL
authors.lastname: Liu
authors.forename: Nai-Zhen
authors.initials: NZ
authors.lastname: You
mesh_terms: D000818:Animals; D001161:Arteriosclerosis / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D002469:Cell Separation; D004036:Diet, Atherogenic; D004958:Estradiol / Q000494:pharmacology* / Q000627:therapeutic use; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D010052:Ovariectomy; D012333:RNA, Messenger / Q000096:biosynthesis / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D023063:Receptors, Interleukin-8B / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger; D023063:Receptors, Interleukin-8B; D004958:Estradiol