title: Anti-conflict-like actions of intralateral septal infusions of allopregnanolone in Wistar rats.
abstract: The aim of the present study was to test the hypothesis that allopregnanolone infused into the lateral septal nuclei will reduce conflict-like behavior in ovariectomized rats. The interaction with systemic administration of several agonists and antagonists of the GABA-A receptor was assessed. Results showed that intralateral septal doses of allopregnanolone (1.0 microg, P<.05; 2.0 microg, P<.05) or systemic injections of allopregnanolone (1.0 mg/kg s.c., P<.05; 2.0 mg/kg s.c., P<.05), diazepam (2.0 mg/kg i.p., P<.05), or muscimol (0.3 mg/kg i.p., P<.05; 0.6 mg/kg i.p., P<.05) reduced conflict-like behavior. Subthreshold doses of intralateral septal infusions of allopregnanolone (0.5 microg/side) synergized with systemic subthreshold doses of GABA-A agonists: allopregnanolone (0.5 mg/kg, P<.05), diazepam (1.5 mg/kg, P<.05), or muscimol (0.1 mg/kg, P<.05). The GABA-A antagonists, flumazenil (0.1 mg/kg i.p.) and bicuculline (2.0 mg/kg i.p.) attenuated the synergism between intralateral septal infusions of allopregnanolone and diazepam or muscimol, respectively. Conversely, neither flumazenil (P<.05) nor bicuculline (P<.05) attenuated the synergism of the combination allopregnanolone (intralateral septum nuclei; 0.5 microg/side) plus systemic injections of allopregnanolone. In conclusion, allopregnanolone reduced conflict-like behavior probably acting at the GABA-A receptors found in the lateral septal nuclei.
authors.affiliation: Laboratorio de Conducta, Instituto de Investigaciones Psicológicas, Universidad Veracruzana, POB 361, Jalapa, Veracruz 91000, Mexico. mimoli@todito.com
authors.forename: M
authors.initials: M
authors.lastname: Molina-Hernández
authors.forename: N P
authors.initials: NP
authors.lastname: Tellez-Alcántara
authors.forename: J
authors.initials: J
authors.lastname: Pérez García
authors.forename: J I
authors.initials: JI
authors.lastname: Olivera Lopez
authors.forename: M
authors.initials: M
authors.lastname: Teresa Jaramillo
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D001640:Bicuculline / Q000494:pharmacology; D003220:Conflict, Psychological; D004305:Dose-Response Relationship, Drug; D005260:Female; D005442:Flumazenil / Q000494:pharmacology; D007839:Functional Laterality / Q000502:physiology*; D018755:GABA Agonists / Q000494:pharmacology; D018756:GABA Antagonists / Q000494:pharmacology; D058785:GABA-A Receptor Agonists; D058787:GABA-A Receptor Antagonists; D012739:Gonadal Steroid Hormones / Q000008:administration & dosage / Q000494:pharmacology*; D010052:Ovariectomy; D011280:Pregnanolone / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D020665:Septum of Brain / Q000033:anatomy & histology / Q000502:physiology*; D013238:Stereotaxic Techniques
chemical_list: D018755:GABA Agonists; D018756:GABA Antagonists; D058785:GABA-A Receptor Agonists; D058787:GABA-A Receptor Antagonists; D012739:Gonadal Steroid Hormones; D005442:Flumazenil; D011280:Pregnanolone; D001640:Bicuculline