title: Functional analysis of the EGF-like domain mutations Pro55Ser and Pro55Leu, which cause mild hemophilia B.
abstract: We studied the functional role of two mutations, Pro55Ser and Pro55Leu, located in the N-terminal Epidermal Growth Factor-like domain (EGF1) of coagulation factor (F) IX. Both mutations cause mild hemophilia B with habitual FIX coagulant activities of 10-12% and FIX antigen levels of 50%. We found that activation by FVIIa/TF and FXIa was normal for FIXPro55Ser, but resulted in proteolysis of FIXPro55Leu at Arg318-Ser319 with a concomitant loss of amidolytic activity, suggesting intramolecular communication between EGF1 and the serine protease domain in FIX. This was further supported by experiments using an anti-EGF1 monoclonal antibody. Activation of FX by FIXaPro55Ser was impaired in both the presence and the absence of phospholipid or FVIIIa, indicating that Pro55 is not directly involved in binding to FVIIIa. We also studied the effect of the two Pro55 mutations on Ca2+ affinity and found only small changes. Thus, the Pro55Ser mutation causes hemophilia primarily through to an impaired ability to activate FX whereas at least in vitro the Pro55Leu defect interferes with the activation of FIX.
authors.affiliation: Department of Pediatrics, Lund University, University Hospital, Malmö, Sweden. karin.knobe@pediatrik.mas.lu.se
authors.forename: K E
authors.initials: KE
authors.lastname: Knobe
authors.forename: K E M
authors.initials: KE
authors.lastname: Persson
authors.forename: E
authors.initials: E
authors.lastname: Sjörin
authors.forename: B O
authors.initials: BO
authors.lastname: Villoutreix
authors.forename: J
authors.initials: J
authors.lastname: Stenflo
authors.forename: R C R
authors.initials: RC
authors.lastname: Ljung
mesh_terms: D002118:Calcium / Q000378:metabolism; D004252:DNA Mutational Analysis; D004815:Epidermal Growth Factor / Q000737:chemistry*; D005164:Factor IX / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015949:Factor IXa / Q000737:chemistry / Q000235:genetics*; D015942:Factor VIIa / Q000378:metabolism; D005170:Factor X / Q000378:metabolism; D002836:Hemophilia B / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D020125:Mutation, Missense; D017434:Protein Structure, Tertiary / Q000502:physiology; D013925:Thromboplastin / Q000378:metabolism
chemical_list: D004815:Epidermal Growth Factor; D005164:Factor IX; D005170:Factor X; D013925:Thromboplastin; D015942:Factor VIIa; D015949:Factor IXa; D002118:Calcium