Tetrabiphenylporphyrin-based receptors for protein surfaces show sub-nanomolar affinity and enhance unfolding.
A family of tetrabiphenylporphyrin-based receptors has been synthesized. Receptor 7 showed sub-nanomolar affinity (K(d)=0.67 nM) in binding to the surface of cytochrome c. In addition, a stoichiometric amount of the receptor 7 caused a lowering in the T(m) of cytochrome c from 85 to 35 degrees C.
Aya
Toshihiro
T
Department of Chemistry, Yale University, New Haven, CT 06520-8107, USA.
Hamilton
Andrew D
AD
D001713:Biphenyl Compounds / Q000138:chemical synthesis*; D002942:Circular Dichroism; D045304:Cytochromes c / Q000737:chemistry*; D008956:Models, Chemical; D011166:Porphyrins / Q000737:chemistry*; D011485:Protein Binding; D017510:Protein Folding; D018000:Receptors, Peptide / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism; D013329:Structure-Activity Relationship; D013696:Temperature
D001713:Biphenyl Compounds; D011166:Porphyrins; D018000:Receptors, Peptide; C409084:tetraphenylporphyrin; D045304:Cytochromes c