title: Biosensors in clinical chemistry.
abstract: Biosensors are analytical devices composed of a recognition element of biological origin and a physico-chemical transducer. The biological element is capable of sensing the presence, activity or concentration of a chemical analyte in solution. The sensing takes place either as a binding event or a biocatalytical event. These interactions produce a measurable change in a solution property, which the transducer converts into a quantifiable electrical signal. Present-day applications of biosensors to clinical chemistry are reviewed, including basic and applied research, commercial applications and fabrication techniques. Recognition elements include enzymes as biocatalytic recognition elements and immunoagents and DNA segments as affinity ligand recognition elements, coupled to electrochemical and optical modes of transduction. The future will include biosensors based on synthetic recognition elements to allow broad applicability to different classes of analytes and modes of transduction extending lower limits of sensitivity. Microfabrication will permit biosensors to be constructed as arrays and incorporated into lab-on-a-chip devices.
authors.affiliation: Instrumentation Laboratory, 101 Hartwell Avenue, Lexington MA 02421, USA. pdorazio@ilww.com
authors.forename: Paul
authors.initials: P
authors.lastname: D'Orazio
mesh_terms: D015374:Biosensing Techniques / Q000639:trends; D002624:Chemistry, Clinical / Q000295:instrumentation* / Q000639:trends; D004247:DNA / Q000737:chemistry; D004563:Electrochemistry; D004800:Enzymes, Immobilized; D005455:Fluorescent Antibody Technique / Q000295:instrumentation; D005947:Glucose / Q000737:chemistry; D006801:Humans; D007120:Immunochemistry / Q000295:instrumentation; D008024:Ligands
chemical_list: D004800:Enzymes, Immobilized; D008024:Ligands; D004247:DNA; D005947:Glucose