title: Programmed adsorption and release of proteins in a microfluidic device.
abstract: A microfluidic device has been developed that can adsorb proteins from solution, hold them with negligible denaturation, and release them on command. The active element in the device is a 4-nanometer-thick polymer film that can be thermally switched between an antifouling hydrophilic state and a protein-adsorbing state that is more hydrophobic. This active polymer has been integrated into a microfluidic hot plate that can be programmed to adsorb and desorb protein monolayers in less than 1 second. The rapid response characteristics of the device can be manipulated for proteomic functions, including preconcentration and separation of soluble proteins on an integrated fluidics chip.
authors.affiliation: Sandia National Laboratory, Post Office Box 5800, Albuquerque, NM 87185-1413, USA.
authors.forename: Dale L
authors.initials: DL
authors.lastname: Huber
authors.forename: Ronald P
authors.initials: RP
authors.lastname: Manginell
authors.forename: Michael A
authors.initials: MA
authors.lastname: Samara
authors.forename: Byung-Il
authors.initials: BI
authors.lastname: Kim
authors.forename: Bruce C
authors.initials: BC
authors.lastname: Bunker
mesh_terms: D000180:Acrylic Resins; D000327:Adsorption; D001671:Biochemistry / Q000295:instrumentation / Q000379:methods*; D003574:Cytochrome c Group / Q000737:chemistry; D006454:Hemoglobins / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D008832:Microchemistry; D008904:Miniaturization; D011506:Proteins / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
chemical_list: D000180:Acrylic Resins; D003574:Cytochrome c Group; D006454:Hemoglobins; D011506:Proteins; C052970:poly-N-isopropylacrylamide; D012710:Serum Albumin, Bovine