Analysis of RNA-protein interactions by flow cytometry.
Flow cytometry, in combination with advances in bead coding technologies, is maturing as a powerful high-throughput approach for analyzing molecular interactions. Applications of this technology include antibody assays and single nucleotide polymorphism mapping. This review describes the recent development of a microbead flow cytometric approach to analyze RNA-protein interactions and discusses emerging bead coding strategies that together will allow genome-wide identification of RNA-protein complexes. The microbead flow cytometric approach is flexible and provides new opportunities for functional genomic studies and small-molecule screening.
Brodsky
Alexander S
AS
Dana-Farber Cancer Institute, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 1 Jimmy Fund Way, SM 922, Boston, MA 02115, USA. alex_brodsky@dfci.harvard.edu
Johnston
Angus P
AP
Trau
Matt
M
Silver
Pamela A
PA
D005434:Flow Cytometry / Q000379:methods*; D023281:Genomics; D006801:Humans; D008863:Microspheres; D020411:Oligonucleotide Array Sequence Analysis; D009841:Oligonucleotides / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D012313:RNA / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013499:Surface Properties
D009841:Oligonucleotides; D011506:Proteins; D011993:Recombinant Fusion Proteins; D012313:RNA