title: Modulation of Ca(v)3.2 T-type Ca2+ channels by protein kinase C.
abstract: Although T-type Ca2+ channels have been implicated in numerous physiological functions, their regulations by protein kinases have been obscured by conflicting reports. We investigated the effects of protein kinase C (PKC) on Ca(v)3.2 T-type channels reconstituted in Xenopus oocytes. Phorbol-12-myristate-13-acetate (PMA) strongly enhanced the amplitude of Ca(v)3.2 channel currents (approximately 3-fold). The augmentation effects were not mimicked by 4alpha-PMA, an inactive stereoisomer of PMA, and abolished by preincubation with PKC inhibitors. Our findings suggest that PMA upregulates Ca(v)3.2 channel activity via activation of oocyte PKC.
authors.affiliation: Department of Life Science, Sogang University, Shinsu-dong, Mapo-Gu, 121-742, Seoul, South Korea.
authors.forename: Jin-Yong
authors.initials: JY
authors.lastname: Park
authors.forename: Seong-Woo
authors.initials: SW
authors.lastname: Jeong
authors.forename: Edward
authors.initials: E
authors.lastname: Perez-Reyes
authors.forename: Jung-Ha
authors.initials: JH
authors.lastname: Lee
mesh_terms: D000818:Animals; D020747:Calcium Channels, T-Type / Q000502:physiology*; D003001:Cloning, Molecular; D006801:Humans; D007700:Kinetics; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009865:Oocytes / Q000187:drug effects / Q000502:physiology; D011493:Protein Kinase C / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014981:Xenopus
chemical_list: C479952:CACNA1H protein, human; D020747:Calcium Channels, T-Type; D011994:Recombinant Proteins; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate