title: Rapid diagnosis of active tuberculosis by detecting antibodies from lymphocyte secretions.
abstract: In the present study, we investigated the tuberculosis (TB) diagnostic performance of an assay on the basis of detection of TB-specific antibodies from peripheral blood mononuclear cells (PBMCs), to determine whether antibodies in lymphocyte secretions obtained from PBMCs would better reflect active disease than antibodies in serum. PBMCs from patients with and without TB cultured in various concentrations for different times were assessed. Immunoglobulin G (IgG) specific for antigen (bacille Calmette-Guérin [BCG] vaccine and purified protein derivative [PPD]) was measured in lymphocyte secretions. Patients with active TB had higher BCG- or PPD-specific IgG antibody responses than patients without TB or healthy subjects (P=.001). This method can be used as a quick diagnostic aid to facilitate rapid detection of TB cases.
authors.affiliation: International Center for Diarrhoeal Disease Research, Bangladesh. rubhana@icddrb.org.
authors.forename: Rubhana
authors.initials: R
authors.lastname: Raqib
authors.forename: Jubayer
authors.initials: J
authors.lastname: Rahman
authors.forename: A K M
authors.initials: AK
authors.lastname: Kamaluddin
authors.forename: S M Mostafa
authors.initials: SM
authors.lastname: Kamal
authors.forename: Fauzia A
authors.initials: FA
authors.lastname: Banu
authors.forename: Shakeel
authors.initials: S
authors.lastname: Ahmed
authors.forename: Zeaur
authors.initials: Z
authors.lastname: Rahim
authors.forename: Pradip K
authors.initials: PK
authors.lastname: Bardhan
authors.forename: Jan
authors.initials: J
authors.lastname: Andersson
authors.forename: David A
authors.initials: DA
authors.lastname: Sack
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000032:analysis*; D000921:Antibody-Producing Cells / Q000276:immunology*; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007074:Immunoglobulin G / Q000032:analysis*; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009163:Mycobacterium bovis / Q000276:immunology; D011237:Predictive Value of Tests; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D013997:Time Factors; D014373:Tuberculin / Q000276:immunology; D014376:Tuberculosis / Q000097:blood / Q000175:diagnosis*
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D014373:Tuberculin