Free radical induced increase in protein carbonyl is attenuated by low dose of adenosine in hippocampus and mid brain: implication in neurodegenerative disorders.
There is strong evidence that oxidative stress participates in the etiology of neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease. In the previous studies we have already shown that a combination of alpha-tocopherol and ascorbic acid protect neurons against tert-butyl hydroperoxide (t-BuOOH) induced neurotoxicity in different brain regions including hippocampus and mid brain. In this work, we examined the neuroprotective effect of low dose of adenosine against protein oxidation (protein carbonyls) in parallel with the level of reduced glutathione (GSH) in hippocampus and mid brain regions of mouse brain. The t-BuOOH was injected intraperitoneally in three concentrations (50, 100, 150 mg/kg b.w.) for 10 days. Results showed dose dependent increase in protein carbonyl (PC) in hippocampus and mid brain region. This increase was accompanied by a significant (p < 0.05) decline in GSH content in both brain regions of t-BuOOH treated mice. Adenosine (1 mg/kg b.w.) protected both hippocampus and mid brain neurons against protein oxidation as evidenced by reduction in protein carbonyl content. The GSH content was significantly (p < 0.05) increased after the treatment of adenosine in both brain regions. These data show that prior treatment with low dose of adenosine attenuates the oxidative protein damage with parallel increase in the GSH level in hippocampus and mid brain of t-BuOOH induced mice.
Parihar
M S
MS
Biochemistry Division, Faculty of Life Sciences, School of Studies in Zoology, Vikram University, Ujjain M.P., India. msparihar@hotmail.com
Pandit
M K
MK
D000241:Adenosine / Q000494:pharmacology*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005609:Free Radicals; D005978:Glutathione / Q000032:analysis / Q000378:metabolism*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008636:Mesencephalon / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D019636:Neurodegenerative Diseases / Q000139:chemically induced / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism*; D011506:Proteins / Q000032:analysis / Q000378:metabolism*; D020122:tert-Butylhydroperoxide
D005609:Free Radicals; D011506:Proteins; D020122:tert-Butylhydroperoxide; D005978:Glutathione; D000241:Adenosine