title: Lactobacillus gasseri OLL2716 as a probiotic in clarithromycin-resistant Helicobacter pylori infection.
abstract: OBJECTIVE
  Clarithromycin (CAM)-resistant Helicobacter pylori sometimes offers serious problems with eradication by antibiotics. The aim of this study was to determine whether a probiotic can be an alternative therapy in CAM-resistant Hp infection.
  
  
  METHODS
  The effects of Lactobacillus gasseri (strain OLL2716) on the growth of CAM-susceptible and CAM-resistant H. pylori and interleukin (IL)-8 production provoked by these strains were examined by in vitro experiments. Moreover, mice were infected with these CAM-susceptible or CAM-resistant H. pylori, and were treated with CAM or L. gasseri.
  
  
  RESULTS
  In vitro experiments demonstrated that L. gasseri inhibited the growth of H. pylori and suppressed H. pylori-associated IL-8 production. Such effects were noted in CAM-resistant and CAM-susceptible H. pylori. Similarly, in an in vivo model of H. pylori infection, H. pylori colonization was significantly decreased by L. gasseri.
  
  
  CONCLUSIONS
  Therefore, L. gasseri was found to act as a probiotic in CAM-resistant H. pylori infection.
authors.affiliation: Department of Infectious Diseases, Tokai University School of Medicine, Bohseidai, Isehara, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ushiyama
authors.forename: Kazuo
authors.initials: K
authors.lastname: Tanaka
authors.forename: Yuji
authors.initials: Y
authors.lastname: Aiba
authors.forename: Tadahiko
authors.initials: T
authors.lastname: Shiba
authors.forename: Atsushi
authors.initials: A
authors.lastname: Takagi
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Mine
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Koga
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017291:Clarithromycin / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D016481:Helicobacter Infections / Q000188:drug therapy*; D016480:Helicobacter pylori / Q000187:drug effects*; D016209:Interleukin-8 / Q000378:metabolism; D007778:Lactobacillus; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008826:Microbial Sensitivity Tests; D019936:Probiotics / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D016209:Interleukin-8; D017291:Clarithromycin