By-product formation by a novel glycerol-producing yeast, Candida glycerinogenes, with different O2 supplies.
Candida glycerinogenes is an aerobe which does not depend on sulphite for production of glycerol. With a sufficient O2 supply, up to 130 g glycerol l(-1) was produced with 2.6 g acetic acid l(-1) as by-product. However, with an insufficient O2 supply--with higher volumes of medium or at higher corn steep liquid concentrations--the glycerol concentration was lower because the by-products, ethanol, pyruvate and lactic acid, were produced in greater amounts, up to 45 g l(-1), 4.3 g l(-1), 1.6 g l(-1), respectively, whereas, less acetic acid (0.6 g l(-1)) was produced. In addition, ethanol decreased to 0.4 g l(-1) and the glycerol yield improved from 34 to 50% (w/w) by adding 50 g sulphite l(-1), nevertheless, acetic acid increased to 7.8 g l(-1).
Jin
Hairu
H
School of Biotechnology, Southern Yangtze University, Huihe Road No. 170, Wuxi, Jiangsu Province 214036, PR China. hairujin160270@yahoo.com
Fang
Huiying
H
Zhuge
Jian
J
D019342:Acetic Acid / Q000378:metabolism; D002175:Candida / Q000145:classification / Q000187:drug effects / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism*; D007656:Ketoglutaric Acids / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D019289:Pyruvic Acid / Q000378:metabolism; D013045:Species Specificity; D013447:Sulfites / Q000494:pharmacology; D003313:Zea mays
D007656:Ketoglutaric Acids; D013447:Sulfites; D019344:Lactic Acid; D000431:Ethanol; D019289:Pyruvic Acid; D005947:Glucose; D005990:Glycerol; D019342:Acetic Acid; D010100:Oxygen