title: BCA-1, A B-cell chemoattractant signal, is constantly expressed in cutaneous lymphoproliferative B-cell disorders.
abstract: We analysed the immunophenotypic and molecular expression of BCA-1 (B-cell-specific chemokine) and CXCR5 (BCA-1 receptor) in normal skin and different cutaneous lymphoproliferative disorders (cutaneous T-cell lymphoma (CTCL); cutaneous B-cell lymphoma (CBCL); cutaneous B-cell pseudolymphoma (PCBCL)), with the aim of investigating their possible involvement in the pathogenesis of cutaneous B-cell disorders. BCA-1 and CXCR5 were constantly expressed in CBCL and PCBCL, but not in normal skin and CTCL. BCA-1 and CXCR5 were constantly coexpressed by CD22+ B-cells, while CD35+ follicular dendritic cells coexpressed BCA-1 in PCBCL cells only. In low grade CBCL, as compared with high grade CBCL, the intensity of CXCR5 expression on neoplastic CD22+ cells was lower than that of BCA-1. The image analysis of reverse transcriptase-polymerase chain reaction (RT-PCR) products showed a significant quantitative difference between PCBCL/low grade CBCL and high grade CBCL. The above findings, although only observed in a small series of patients, are in keeping with findings in MALT gastric and gastric MALT lymphomas, adding further evidence of the close similarities between CBCL and MALT lymphomas.
authors.affiliation: Department of Dermatological Sciences, University of Florence Medical School, Firenze, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Mori
authors.forename: C
authors.initials: C
authors.lastname: Manuelli
authors.forename: N
authors.initials: N
authors.lastname: Pimpinelli
authors.forename: B
authors.initials: B
authors.lastname: Bianchi
authors.forename: C
authors.initials: C
authors.lastname: Orlando
authors.forename: C
authors.initials: C
authors.lastname: Mavilia
authors.forename: P
authors.initials: P
authors.lastname: Cappugi
authors.forename: E
authors.initials: E
authors.lastname: Maggi
authors.forename: B
authors.initials: B
authors.lastname: Giannotti
authors.forename: M
authors.initials: M
authors.lastname: Santucci
mesh_terms: D001483:Base Sequence; D054382:Chemokine CXCL13; D019743:Chemokines, CXC / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D016393:Lymphoma, B-Cell / Q000378:metabolism*; D016410:Lymphoma, T-Cell, Cutaneous / Q000378:metabolism*; D008969:Molecular Sequence Data; D012334:RNA, Neoplasm / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012878:Skin Neoplasms / Q000378:metabolism*
chemical_list: C110663:CXCL13 protein, human; D054382:Chemokine CXCL13; D019743:Chemokines, CXC; D012334:RNA, Neoplasm