title: EF loop conformational change triggers ligand binding in beta-lactoglobulins.
abstract: Beta-lactoglobulins, belonging to the lipocalin family, are a widely studied group of proteins, characterized by the ability to solubilize and transport hydrophobic ligands, especially fatty acids. Despite many reports, the mechanism of ligand binding and the functional role of these proteins is still unclear, and many contradicting concepts are often encountered in the literature. In the present paper the comparative analysis of the binding properties of beta-lactoglobulins has been performed using sequence-derived information, structure-based electrostatic calculations, docking simulations, and NMR experiments. Our results reveal for the first time the mechanism of beta-lactoglobulin ligand binding, which is completely determined by the opening-closing of EF loop, triggered by Glu89 protonation. The alkaline shift observed for Glu89 pKa in porcine beta-lactoglobulin (pKa 9.7) with respect to the bovine species (pKa 5.5) depends upon the interplay of electrostatic effects of few nearby key residues. Porcine protein is therefore able to bind fatty acids provided that the appropriate pH solution conditions are met (pH > 8.6), where the EF loop conformational change can take place. The unusually high pH of binding detected for porcine beta-lactoglobulin seems to be functional to lipases activity. Theoretical pKa calculations extended to representative beta-lactoglobulins allowed the identification of key residues involved in structurally and functionally important electrostatic interactions. The results presented here provide a strong indication that the described conformational change is a common feature of all beta-lactoglobulins.
authors.affiliation: Laboratorio Risonanza Magnetica Nucleare, Istituto Macromolecole, Consiglio Nazionale delle Ricerche, via Bassini 15, 20133 Milano, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Ragona
authors.forename: Federico
authors.initials: F
authors.lastname: Fogolari
authors.forename: Maddalena
authors.initials: M
authors.lastname: Catalano
authors.forename: Raffaella
authors.initials: R
authors.lastname: Ugolini
authors.forename: Lucia
authors.initials: L
authors.lastname: Zetta
authors.forename: Henriette
authors.initials: H
authors.lastname: Molinari
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D005227:Fatty Acids / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007782:Lactoglobulins / Q000737:chemistry* / Q000378:metabolism; D008024:Ligands; D008239:Lysine / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D013552:Swine
chemical_list: D005227:Fatty Acids; D007782:Lactoglobulins; D008024:Ligands; D008239:Lysine