title: Movement of cysteine in intact monkey lenses: the major site of entry is the germinative region.
abstract: Monkey lenses were incubated with 35S-L-cysteine for various times and the movement of label within the lens followed by autoradiography. Cysteine appeared to enter primarily at the germinative region of the lens. No evidence was found for major transport through either the anterior or posterior faces of the lens. The movement of cysteine within different parts of the lens was followed over time. The data suggest that, for cysteine, the major pathway for transport within the lens involves entry at the germinative region followed by movement along the fibre cells. The data were consistent with orthogonal movement across the fibres in the equatorial plane but little or no movement across the fibres at the anterior pole or posterior faces of the lens. Such a scenario is in accord with the distribution of connexons, indicating that this pattern of entry may also be observed for other small molecules. The finding of high permeability at the lens germinative region is in accord with the anatomy of the eye, since this is the lens surface in contact with the posterior chamber. Thus, cysteine secreted by the ciliary body into the aqueous humor would come into contact initially with the region of the lens best able to absorb this amino acid. Although this aspect was not addressed in the current study, the same phenomenon may also be observed with other lens nutrients.
authors.affiliation: Save Sight Institute, University of Sydney, NSW 2006, Australia.
authors.forename: Matthew H J
authors.initials: MH
authors.lastname: Sweeney
authors.forename: Donita L
authors.initials: DL
authors.lastname: Garland
authors.forename: Roger J W
authors.initials: RJ
authors.lastname: Truscott
mesh_terms: D000818:Animals; D001345:Autoradiography; D003545:Cysteine / Q000493:pharmacokinetics*; D005901:Glaucoma / Q000209:etiology; D007908:Lens, Crystalline / Q000378:metabolism*; D008253:Macaca mulatta
chemical_list: D003545:Cysteine