title: Detailed Hylobates lar karyotype defined by 25-color FISH and multicolor banding.
abstract: A comprehensive and detailed comparative chromosome map of the white-handed gibbon (Hylobates lar = HLA) has been established by hybridizing the recently developed complete human multicolor banding (MCB) probe set on metaphase chromosomes of a male HLA lymphoblastoid cell line. Thus, it was possible to precisely determine the breakpoints and distribution plus orientation of specific DNA-regions in this cytogenetically highly rearranged species compared to Homo sapiens (HSA). In general, the obtained results are in concordance with previous molecular-cytogenetic studies. In this study all 71 breakpoints present in HLA compared to HSA could be determined exactly. This study is a valuable complement to our knowledge on the phylogeny of huminoid chromosomes.
authors.affiliation: Institute of Human Genetics and Anthropology, D-07740 Jena, Germany.
authors.forename: Kristin
authors.initials: K
authors.lastname: Mrasek
authors.forename: Anita
authors.initials: A
authors.lastname: Heller
authors.forename: Nikolai
authors.initials: N
authors.lastname: Rubtsov
authors.forename: Vladimir
authors.initials: V
authors.lastname: Trifonov
authors.forename: Heike
authors.initials: H
authors.lastname: Starke
authors.forename: Uwe
authors.initials: U
authors.lastname: Claussen
authors.forename: Thomas
authors.initials: T
authors.lastname: Liehr
mesh_terms: D000818:Animals; D002871:Chromosome Banding / Q000379:methods*; D019457:Chromosome Breakage; D020223:Chromosome Painting; D003116:Color; D015342:DNA Probes / Q000235:genetics; D006801:Humans; D006922:Hylobates / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007621:Karyotyping; D008297:Male
chemical_list: D015342:DNA Probes