title: Quantitative trait loci conditioning transovarial transmission of La Crosse virus in the eastern treehole mosquito, Ochlerotatus triseriatus.
abstract: Quantitative trait loci (QTL) affecting the ability of the Eastern treehole mosquito, Ochlerotatus triseriatus, to transovarially transmit (TOT) La Crosse virus (LAC) were mapped in an F1 intercross. The Holmen strain of O. triseriatus, previously selected for TOT refractoriness, was crossed to the AIDL strain that had been selected for TOT permissiveness. In P1 and F1 parents and 49 F2 individuals, regions of 10 cDNA loci were analysed with single strand conformation polymorphism (SSCP) analysis to identify and orientate linkage groups. Genotypes were also scored at fifty-six random amplified polymorphic DNA (RAPD)-SSCP loci. Twenty-eight F2 offspring were individually analysed for TOT. Three QTL for TOT were detected with standard interval mapping on chromosomes II and III. Alleles at the three loci contributed additively towards determining the overall TOT rate and cumulatively accounted for approximately 53% of the phenotypic variance in TOT.
authors.affiliation: Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523-1677, USA.
authors.forename: Douglas H
authors.initials: DH
authors.lastname: Graham
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Holmes
authors.forename: Barry J
authors.initials: BJ
authors.lastname: Beaty
authors.forename: William C
authors.initials: WC
authors.lastname: Black
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D009033:Culicidae / Q000235:genetics* / Q000821:virology*; D004670:Encephalitis, California / Q000635:transmission* / Q000821:virology; D005260:Female; D008040:Genetic Linkage / Q000235:genetics*; D018445:Infectious Disease Transmission, Vertical; D007303:Insect Vectors / Q000235:genetics; D018062:La Crosse virus / Q000254:growth & development*; D008297:Male; D018807:Polymorphism, Single-Stranded Conformational; D040641:Quantitative Trait Loci / Q000235:genetics*; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D012367:RNA, Viral