The culture and establishment of embryonic germ (EG) cell lines from Chinese mini swine.
As a part of a basic research project on Xeno-transplantion, we have been engaged in the derivation of embryonic stem cell lines from Chinese mini swine. Here, we reported for the first time the establishment of two porcine EG cell lines (BPEG1 and BPEG2) from primordial germ cells of genital ridges of a 28 and a 27 d embryos respectively. Their pluripotent nature has been identified by colony morphology, marker characterization as well as by in vitro and in vivo differentiation. These porcine EG cells are potentially useful for further basic studies.
Tsung
Hsiao Chien
HC
Laboratory of Embryonic Stem Cell, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China. escg@sunm.shcnc.ac.cn
Du
Zhong Wei
ZW
Rui
Rong
R
Li
Xiu Lan
XL
Bao
Lin Ping
LP
Wu
Jun
J
Bao
Shi Min
SM
Yao
Zhen
Z
D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D000954:Antigens, Surface / Q000276:immunology; D015415:Biomarkers / Q000032:analysis; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000502:physiology; D004268:DNA-Binding Proteins / Q000276:immunology; D004622:Embryo, Mammalian / Q000737:chemistry / Q000166:cytology* / Q000648:ultrastructure; D005260:Female; D005854:Germ Cells / Q000737:chemistry / Q000166:cytology* / Q000648:ultrastructure; D007150:Immunohistochemistry; D007621:Karyotyping; D008297:Male; D008854:Microscopy, Electron; D050814:Octamer Transcription Factor-3; D013234:Stem Cells / Q000737:chemistry / Q000166:cytology / Q000648:ultrastructure; D013552:Swine; D013556:Swine, Miniature; D014157:Transcription Factors
D000954:Antigens, Surface; D015415:Biomarkers; D004268:DNA-Binding Proteins; D050814:Octamer Transcription Factor-3; D014157:Transcription Factors; D000469:Alkaline Phosphatase