Chromosomal aberrations in squamous cell carcinoma and solar keratoses revealed by comparative genomic hybridization.
OBJECTIVE
To identify chromosomal copy numbers of frequent genetic aberrations within squamous cell carcinomas (SCCs) and solar keratoses (SKs), and provide further evidence to support or challenge current dogma concerning the relationship between these lesions.


METHODS
Retrospective analysis of genetic aberrations in DNA from SK and SCC biopsy specimens by comparative genomic hybridization.


METHODS
University-based research laboratory in Queensland, Australia.


METHODS
Twenty-two biopsy specimens from patients with diagnosed SKs (n = 7), cutaneous SCCs (n = 10), or adjoining lesions (n = 5).


METHODS
Identification of frequent genetic aberrations both specific to SK and SCC and shared by these lesions to investigate their clonal relationship.


RESULTS
Shared genomic imbalances were identified in SK and SCC. Frequent gains were located at chromosome arms 3q, 17q, 4p, 14q, Xq, 5p, 9q, 8q, 17p, and 20q, whereas shared regional losses were observed at 9p, 3p, 13q, 17p, 11p, 8q, and 18p. Significant loss of 18q was observed only in SCC lesions.


CONCLUSIONS
Our results demonstrate that numerous chromosomal aberrations are shared by the 2 lesions, suggesting a clonal relationship between SK and SCC. Additionally, the genomic loss of 18q may be a significant event in SK progression to SCC. Finally, the type and frequency of aberrations suggests a common mode of tumorigenesis in SCC-derived tumors.
Ashton
Kevin J
KJ
Genomics Research Centre, Griffith University-Gold Coast, Bundall, Queensland, Australia.
Weinstein
Stephen R
SR
Maguire
David J
DJ
Griffiths
Lyn R
LR
D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D002869:Chromosome Aberrations; D004247:DNA / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D006801:Humans; D007642:Keratosis / Q000209:etiology / Q000235:genetics* / Q000473:pathology; D019656:Loss of Heterozygosity; D008297:Male; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies; D012867:Skin / Q000473:pathology; D012878:Skin Neoplasms / Q000235:genetics* / Q000473:pathology; D013472:Sunlight / Q000009:adverse effects*
D004273:DNA, Neoplasm; D004247:DNA