title: Splenic T cells from mice receiving intracameral antigen suppress in-vitro antigen-induced proliferation and interferon-gamma production by sensitized lymph node cells.
abstract: OBJECTIVE
  To characterize the immunoregulatory mechanisms in vitro of spleen cells that are activated by intracameral injection of antigen (AC-spleen cells).
  
  
  METHODS
  AC-spleen cell regulation of in-vitro antigen-induced proliferation and interferon-gamma production by lymph node cells from TNP-BSA-immunized mice was quantified by co-culture of the lymph node cells with TNP-BSA and AC-spleen cells induced by intracameral TNP-BSA. Cytokine production was quantified by ELISA.
  
  
  RESULTS
  AC-spleen cells produced significantly more IL-4 and IL-10 than spleen cells from TNP-BSA-immunized mice or naive spleen cells; unlike spleen cells from immunized mice, AC-spleen cells did not produce IFN-gamma. AC-splenic CD4(+), CD8(+), CD4(-)/CD8( -) (DN) T cells differentially suppressed antigen-induced proliferation and IFN-gamma production by immunized lymph node cells by a mechanism dependent on IL-10 and antigen. Cultures of lymph node cells, antigen, and AC-splenic T cells contained increased amounts of IL-10 and/or TGFbeta2.
  
  
  CONCLUSIONS
  The differential, cytokine-dependent immunoregulatory effects of CD4( +) and CD8(+) AC-spleen cells observed in vitro parallel their effects in vivo. We suggest that the suppression of antigen-induced lymphocyte proliferation and IFN-gamma production by AC-spleen cells provides a useful in-vitro assay of the immunoregulatory activity of cell populations that are induced by the injection of antigen into the anterior chamber.
authors.affiliation: Department of Pathology, Vision Immunology Center, University of Connecticut Health Center, Farmington, CT, USA.
authors.forename: Yafei
authors.initials: Y
authors.lastname: Wang
authors.forename: Wa-el
authors.initials: WE
authors.lastname: Ghali
authors.forename: Pavani
authors.initials: P
authors.lastname: Pingle
authors.forename: Amar
authors.initials: A
authors.lastname: Traboulsi
authors.forename: Tajas
authors.initials: T
authors.lastname: Dalal
authors.forename: James
authors.initials: J
authors.lastname: O'Rourke
authors.forename: Robert E
authors.initials: RE
authors.lastname: Cone
mesh_terms: D000818:Animals; D000867:Anterior Chamber / Q000276:immunology*; D000941:Antigens / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D018920:Coculture Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D018189:Immunomagnetic Separation; D016130:Immunophenotyping; D007165:Immunosuppression Therapy; D007267:Injections; D007371:Interferon-gamma / Q000096:biosynthesis*; D008198:Lymph Nodes / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D012710:Serum Albumin, Bovine / Q000276:immunology; D013154:Spleen / Q000166:cytology*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000941:Antigens; C038601:trinitrophenyl-bovine serum albumin; D012710:Serum Albumin, Bovine; D007371:Interferon-gamma