Biomass segregation in sage cell suspension culture.
The biomass of sage (Salvia officinalis L.) cell suspension culture was composed of single cells and cell aggregates. The development of aggregated cell culture from a single-cell suspension was monitored by particle size distribution for four particle size classes. Particle size distribution was compared between the biomass grown in bioreactor and shake flasks. The size of the particles had a strong influence on content of secondary metabolite, ursolic acid (UA). The single cell biomass fraction accumulated up to 7.7 mg UA g(-1) DW which was up to 50 times higher compared to aggregated biomass fractions.
Bolta
Ziga
Z
Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia.
Baricevic
Dea
D
Raspor
Peter
P
D018533:Biomass; D019149:Bioreactors; D002449:Cell Aggregation / Q000502:physiology; D002452:Cell Count / Q000379:methods; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D010316:Particle Size; D018520:Plant Shoots / Q000166:cytology / Q000254:growth & development / Q000378:metabolism / Q000502:physiology; D011786:Quality Control; D027543:Salvia officinalis / Q000166:cytology / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D014315:Triterpenes / Q000378:metabolism*; D000097245:Ursolic Acid
D014315:Triterpenes; D000097245:Ursolic Acid