title: Construction and expression of recombined human AFP eukaryotic expression vector.
abstract: OBJECTIVE
  To construct a recombined human AFP eukaryotic expression vector for the purpose of gene therapy and target therapy of hepatocellular carcinoma (HCC).
  
  
  METHODS
  The full length AFP-cDNA of prokaryotic vector was digested, and subcloned to the multi-clony sites of the eukaryotic vector. The constructed vector was confirmed by enzymes digestion and electrophoresis, and the product expressed was detected by electrochemiluminescence and immunofluorescence methods.
  
  
  RESULTS
  The full length AFP-cDNA successfully cloned to the eukaryotic vector through electrophoresis, 0.9723 IU/ml AFP antigen was detected in the supernatant of AFP-CHO by electrochemiluminescence method. Compared with the control groups, the differences were significant (P<0.05). AFP antigen molecule was observed in the plasma of AFP-CHO by immunofluorescence staining.
  
  
  CONCLUSIONS
  The recombined human AFP eukaryotic expression vector can express in CHO cell line. It provides experimental data for gene therapy and target therapy of hepatocellular carcinoma.
authors.affiliation: Department of Oncology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China.
authors.forename: Li-Wang
authors.initials: LW
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Ren
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Zhang
authors.forename: Bin
authors.initials: B
authors.lastname: Jin
authors.forename: Bo-Rong
authors.initials: BR
authors.lastname: Pan
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Si
authors.forename: Yan-Jun
authors.initials: YJ
authors.lastname: Zhang
authors.forename: Zhong-Hua
authors.initials: ZH
authors.lastname: Wang
authors.forename: Yang-Lin
authors.initials: YL
authors.lastname: Pan
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Festein
mesh_terms: D000818:Animals; D016466:CHO Cells; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000628:therapy*; D006224:Cricetinae; D018076:DNA, Complementary; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000628:therapy*; D011994:Recombinant Proteins / Q000235:genetics*; D014162:Transfection; D000509:alpha-Fetoproteins / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D011994:Recombinant Proteins; D000509:alpha-Fetoproteins