title: Glycerophosphate-dependent peroxide production by brown fat mitochondria from newborn rats.
abstract: Glycerophosphate (GP)-dependent, ferricyanide-induced hydrogen peroxide production was studied in brown adipose tissue mitochondria from newborn rats. Relations between the rate of hydrogen peroxide production and total amount of hydrogen peroxide produced at different GP and ferricyanide concentrations were determined. It was found that the rate of hydrogen peroxide production increases with increasing GP concentration and decreases with increasing ferricyanide concentration. Total amount of hydrogen peroxide produced increases with increasing ferricyanide concentration, however, not proportionally, and the efficiency of this process (oxygen/ferricyanide ratio) strongly declines. Data presented provide further information on the character and kinetics of hydrogen peroxide production by mammalian mitochondrial glycerophosphate dehydrogenase.
authors.affiliation: Center of Molecular Genomics, Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic. Drahota@biomed.cas.cz
authors.forename: Z
authors.initials: Z
authors.lastname: Drahota
authors.forename: H
authors.initials: H
authors.lastname: Rauchova
authors.forename: P
authors.initials: P
authors.lastname: Jesina
authors.forename: A
authors.initials: A
authors.lastname: Vojtísková
authors.forename: J
authors.initials: J
authors.lastname: Houstek
mesh_terms: D002001:Adipose Tissue, Brown / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D019069:Cell Respiration / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D005292:Ferricyanides / Q000494:pharmacology*; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D005994:Glycerophosphates / Q000378:metabolism* / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005292:Ferricyanides; D005994:Glycerophosphates; C007931:hexacyanoferrate III; D006861:Hydrogen Peroxide; D005993:Glycerolphosphate Dehydrogenase