In-process control of midazolam synthesis by HPLC.
A high-performance liquid chromatographic assay coupled with UV detection (239 nm) has been developed for the determination of midazolam and its synthesis precursors. The separation of the analytes was performed on a Kromasil C8 column (15 cm x 4.6 mm i.d., 5 microm) at 30 degrees C. The mobile phase [ammonium chloride (pH 5.5, 1 g l(-1))-methanolacetonitrile (45:22:33, v/v/v)] was pumped at a flow-rate of 1.5 ml min(-1). This method is rapid (less than 11 min), sensitive (limit of detection (LOD) ranged between 0.05 and 0.5 mg l(-1)) and selective for the determination of midazolam, and it could be used for monitoring different synthetic routes.
Laviana
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L
Asturpharma S.A., c/ Peña Brava, 22B-23, Silvota, 33192 Llanera, Asturias, Spain.
Llorente
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I
Bayod
M
M
Blanco
D
D
D002851:Chromatography, High Pressure Liquid; D006993:Hypnotics and Sedatives / Q000138:chemical synthesis / Q000737:chemistry*; D017895:Manganese Compounds / Q000737:chemistry; D008874:Midazolam / Q000138:chemical synthesis / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
D006993:Hypnotics and Sedatives; D017895:Manganese Compounds; D010087:Oxides; D008874:Midazolam; C016552:manganese dioxide