High cell density cultivation of Brevibacterium linens and formation of proteinases and lipase.
Brevibacterium linens forms hydrolytic enzymes which can be used to accelerate the ripening of cheese without causing bitterness. B. linens ATCC 9172 was grown to a high cell density (50 g dry wt l-1 after 60 h) in a mineral medium containing lactic acid, soy-peptone and ammonium sulphate by applying a continuous feed of nutrients. The maximal activities of L-leucine aminopeptidase and cell-associated proteinase were 286 U l-1 and 202 U l-1, respectively. The cell-associated lipolytic activity exhibited a strong and sudden increase at 46 h, resulting in a maximum of 9.5 U g-1 dry wt; thus the volumetric productivity of proteolytic and lipolytic activity was 4220 U l-1 h-1 and 7.3 U l-1 h-1, respectively.
Adamitsch
Bernhard F
BF
Institute of Chemical Engineering, Vienna University of Technology, Getreidemarkt 9/166, A-1060 Vienna, Austria. bernhard.adamitsch@tuwien.ac.at
Karner
Ferdinand
F
Hampel
Werner A
WA
D000645:Ammonium Sulfate / Q000494:pharmacology; D001951:Brevibacterium / Q000145:classification / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development*; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D010450:Endopeptidases / Q000096:biosynthesis; D004789:Enzyme Activation; D019344:Lactic Acid / Q000494:pharmacology; D007931:Leucyl Aminopeptidase / Q000096:biosynthesis; D008049:Lipase / Q000096:biosynthesis*; D010461:Peptones / Q000494:pharmacology; D011786:Quality Control; D030262:Soybean Proteins / Q000494:pharmacology; D013045:Species Specificity
D003470:Culture Media; D010461:Peptones; D030262:Soybean Proteins; D019344:Lactic Acid; D008049:Lipase; D010450:Endopeptidases; D007931:Leucyl Aminopeptidase; D000645:Ammonium Sulfate