title: Inactivation of Ebola virus with a surfactant nanoemulsion.
abstract: Hemorrhagic fever caused by Ebola virus (EBO) is a highly contagious infection. This necessitates that the contaminated instruments, clothes, and hospital premises must be completely disinfected. Nanoemulsions are a new form of disinfectant composed of detergents and vegetable oil suspended in water. The antiviral activity of nanoemulsion ATB has been investigated against EBO. The nanoemulsion was tested against two preparations of EBO (strain Zaire) obtained from Vero cell culture fluid (EBO-zc) and from blood of infected monkeys (EBO-zb). The nanoemulsion ATB was virucidal against both preparations of EBO, inactivating the purified virus within 20 min even when diluted 1:100 with the growth medium. Inactivation of the virus in tissue preparations was also complete, but required 1:10 dilutions with media or higher. After treatment with ATB (10 and 1% concentrations), no EBO was apparent even after two passages in Vero cell culture. These data indicate that the nanoemulsion is an effective disinfectant for EBO. Because of the excellent biocompatibility of nanoemulsions, studies are planned to determine whether the nanoemulsion-killed virus is suitable for developing a vaccine against EBO.
authors.affiliation: State Research Center of Virology and Biotechnology Vector, Koltsovo, Novosibirsk region 630559, Russia. chepurnov@vector.nsc.ru
authors.forename: A A
authors.initials: AA
authors.lastname: Chepurnov
authors.forename: L F
authors.initials: LF
authors.lastname: Bakulina
authors.forename: A A
authors.initials: AA
authors.lastname: Dadaeva
authors.forename: E N
authors.initials: EN
authors.lastname: Ustinova
authors.forename: T S
authors.initials: TS
authors.lastname: Chepurnova
authors.forename: J R
authors.initials: JR
authors.lastname: Baker
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D004203:Disinfection / Q000379:methods*; D029043:Ebolavirus / Q000187:drug effects* / Q000254:growth & development; D004655:Emulsions; D019142:Hemorrhagic Fever, Ebola / Q000517:prevention & control*; D010938:Plant Oils; D013501:Surface-Active Agents; D014709:Vero Cells
chemical_list: D004655:Emulsions; D010938:Plant Oils; D013501:Surface-Active Agents