title: Evaluation of reproductive development following intravenous and oral exposure to DEHP in male neonatal rats.
abstract: Di-(2-ethylhexyl)phthalate (DEHP) was administered to 3- to 5-day-old male Sprague-Dawley rats by daily intravenous injections of 60, 300, or 600 mg/kg/day or by daily oral gavage of 300 or 600 mg/kg/day for 21 days. Histopathological evaluation and organ weight measurements were performed on some animals after 21 days of dosing (primary group) and later on the recovery group animals that were held without further treatment until sexual maturity at approximately 90 days of age. No effects of any type were observed in animals treated intravenously with 60 mg/kg/day. Testicular changes, consisting of a partial depletion of the germinal epithelium and/or decrease in diameter of seminiferous tubules, were present in all animals of the 300- and 600-mg/kg/day groups after the 21-day dosing period. Testes weight decreased and liver weight increased in these animals. Testes changes were dose-related and generally more severe among animals dosed orally versus intravenously. In the recovery animals, a residual DEHP-induced decrease in seminiferous tubule diameter was present in the testis of several animals dosed orally at 300 and 600 mg/kg/day, but not in animals dosed intravenously. There was no germinal cell depletion or Sertoli cell alteration observed in any dose group at any time. Notably, no effects on sperm count, sperm morphology, or sperm motility were observed at 90 days of age in any of the groups.
authors.affiliation: Baxter Healthcare Corporation, Round Lake, Illinois 60073, USA. Jon_Cammack@baxter.com
authors.forename: Jon N
authors.initials: JN
authors.lastname: Cammack
authors.forename: Randy D
authors.initials: RD
authors.lastname: White
authors.forename: Donovan
authors.initials: D
authors.lastname: Gordon
authors.forename: Jerome
authors.initials: J
authors.lastname: Gass
authors.forename: Lawrence
authors.initials: L
authors.lastname: Hecker
authors.forename: David
authors.initials: D
authors.lastname: Conine
authors.forename: Uma Swamy
authors.initials: US
authors.lastname: Bruen
authors.forename: Mitchell
authors.initials: M
authors.lastname: Friedman
authors.forename: Charles
authors.initials: C
authors.lastname: Echols
authors.forename: Tony Y
authors.initials: TY
authors.lastname: Yeh
authors.forename: Daniel M
authors.initials: DM
authors.lastname: Wilson
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000831:Animals, Newborn; D004051:Diethylhexyl Phthalate / Q000008:administration & dosage / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D007275:Injections, Intravenous; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function; D012098:Reproduction / Q000187:drug effects* / Q000502:physiology; D012671:Seminiferous Tubules / Q000187:drug effects / Q000473:pathology; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D013737:Testis / Q000187:drug effects* / Q000254:growth & development / Q000473:pathology; D018675:Toxicity Tests
chemical_list: D004051:Diethylhexyl Phthalate