Late ischemic preconditioning of the myocardium alters the expression of genes involved in inflammatory response.
Myocardial ischemic preconditioning (IPC) is a potent endogenous mechanism of cardioprotection against ischemia-reperfusion injury. In this study we focused on the second phase of IPC as the most interesting in terms of therapeutic implementations. We aimed at the detection of genes, which are differentially expressed at 16 h after reperfusion. Preconditioning of canine myocardium was initiated by 5 min occlusion of the left anterior descending coronary artery with subsequent reperfusion. cDNA representational difference analysis in combination with microarray hybridization and reverse transcription polymerase chain reaction were used to reveal the changes in gene expression in canine hearts. We found that functionally related genes for tristetraproline (TTP), selectin E, matrix metalloproteinase 9, and tumor necrosis factor-alpha were highly upregulated at the late phase of IPC. The upregulation of TTP gene at the late phase of IPC, reported here for the first time, may represent a cardioprotective mechanism, which could be a promising perspective in clinical interventions against ischemia-reperfusion injuries of the heart.
Zubakov
Dmitrij
D
Functional Genome Analysis, German Cancer Research Center (DKFZ Heidelberg), Im Neuenheimer Feld 580, D-69120, Heidelberg, Germany. d.zubakov@dkfz-heidelberg.de <d.zubakov@dkfz-heidelberg.de>
Hoheisel
Jörg D
JD
Kluxen
Franz-Werner
FW
Brändle
Marian
M
Ehring
Thomas
T
Hentsch
Bernd
B
Frohme
Marcus
M
D000818:Animals; D004285:Dogs; D019040:E-Selectin / Q000235:genetics; D005786:Gene Expression Regulation / Q000502:physiology*; D007249:Inflammation / Q000235:genetics* / Q000503:physiopathology; D019157:Ischemic Preconditioning, Myocardial; D020780:Matrix Metalloproteinase 9 / Q000235:genetics; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D019040:E-Selectin; D014409:Tumor Necrosis Factor-alpha; D020780:Matrix Metalloproteinase 9