title: Creatine phosphate--creatine kinase in enzymatic synthesis of glycoconjugates.
abstract: [reaction: see text] Enzymatic production of glycoconjugates is hampered by expensive phosphagens such as acetyl phosphate (AcP) and phosphoenolpyruvate (PEP). Here, we introduce creatine phosphate--creatine kinase system as a novel and practical energy source in carbohydrate synthesis. This system was successfully demonstrated in the production of bioactive oligosaccharides with different sugar nucleotide regeneration systems.
authors.affiliation: Department of Chemistry, Wayne State University, Detroit, Michigan 48202, USA.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Zhang
authors.forename: Bingyuan
authors.initials: B
authors.lastname: Wu
authors.forename: Yingxin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Przemyslaw
authors.initials: P
authors.lastname: Kowal
authors.forename: Peng George
authors.initials: PG
authors.lastname: Wang
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D002384:Catalysis; D002625:Chemistry, Organic / Q000191:economics / Q000379:methods; D003402:Creatine Kinase / Q000737:chemistry* / Q000378:metabolism; D004734:Energy Metabolism; D006001:Glycoconjugates / Q000138:chemical synthesis*; D010725:Phosphocreatine / Q000737:chemistry* / Q000378:metabolism; D013816:Thermodynamics
chemical_list: D006001:Glycoconjugates; D010725:Phosphocreatine; D000255:Adenosine Triphosphate; D003402:Creatine Kinase