Enzymatic determination of pyrophosphate in urine by flow methods.
Two flow methods for the enzymatic determination of pyrophosphate are described that are used to diminish the consumption of reagents. One method is based on the use of an open-close circuit with manual injection using a syringe. The other is a sequential injection method. The analytical features of both methods are: a linear range of 0.4 - 20 mg L(-1), an LOD of 0.38 mg L(-1), and a CV of 2.0% for the sequential injection method, and a linear range of 0.3 - 15 mg L(-1), an LOD of 0.29 mg L(-1), and CV of 2.2% for the open-close circuit method. The methods were applied to the determination of pyrophosphate in urine. The pyrophosphate concentration determined in urine samples varied from 1.26 to 6.67 mg L(-1).
Simonet
Bartolomé M
BM
Department of Chemistry, University of Balearic Islands, E 07071 Palma de Mallorca, Spain.
Grases
Félix
F
March
Juan G
JG
D011756:Diphosphates / Q000378:metabolism* / Q000652:urine*; D004798:Enzymes / Q000378:metabolism; D017022:Flow Injection Analysis / Q000379:methods*; D006801:Humans
D011756:Diphosphates; D004798:Enzymes