Characterization of a type I regulatory subunit of cAMP-dependent protein kinase from the bivalve mollusk Mytilus galloprovincialis.
Two isoforms of the regulatory subunit (R) of cAMP-dependent protein kinase (PKA), named R(myt1) and R(myt2), had been purified in our laboratory from two different tissues of the sea mussel Mytilus galloprovincialis. In this paper, we report the sequences of several peptides obtained from tryptic digestion of R(myt1). As a whole, these sequences showed high homology with regions of type I R subunits from invertebrate and also from mammalian sources, but homology with those of fungal and type II R subunits was much lower, which indicates that R(myt1) can be considered as a type I R isoform. This conclusion is also supported by the following biochemical properties: (1) R(myt1) was proved to have interchain disulfide bonds stabilizing its dimeric structure; (2) it failed to be phosphorylated by the catalytic (C) subunit purified from mussel; (3) it has a higher pI value than that of the R(myt2) isoform; and (4) it showed cross-reactivity with mammalian anti-RIbeta antibody.
Díaz-Enrich
María J
MJ
Departamento de Bioqui;mica e Bioloxi;a Molecular, Facultade de Veterinaria, Universidade de Santiago de Compostela, Campus de Lugo, 27002, Lugo, Spain
Ibarguren
Izaskun
I
Hellman
Ulf
U
Villamarín
J Antonio
JA
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D049872:Bivalvia / Q000201:enzymology*; D000242:Cyclic AMP / Q000378:metabolism; D054746:Cyclic AMP-Dependent Protein Kinase Type II; D017868:Cyclic AMP-Dependent Protein Kinases / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D004220:Disulfides / Q000737:chemistry; D007526:Isoelectric Point; D007527:Isoenzymes / Q000378:metabolism; D008969:Molecular Sequence Data; D021122:Protein Subunits / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid
D004220:Disulfides; D007527:Isoenzymes; D021122:Protein Subunits; D000242:Cyclic AMP; D054746:Cyclic AMP-Dependent Protein Kinase Type II; D017868:Cyclic AMP-Dependent Protein Kinases