title: Rapid preparation of DNA microarray using PCR for hepatitis B and D virus detection.
abstract: OBJECTIVE
  To prepare DNA microarray for detecting both hepatitis B and D virus as (HBV and HDV).
  
  
  METHODS
  With the assistance of Oligo6.4 software, specific PCR primers targeting the conserved region of HBV and HDV were designed. The PCR products were purified and cloned into the pMD18-T vectors, followed by rapid identification. The recombinant plasmids were then extracted from positive clones and the target gene fragments underwent sequence analysis.
  
  
  RESULTS
  The gene fragments of HBV and HDV were obtained by PCR, which were confirmed by sequence analysis to be specific gene fragments of HBV and HDV.
  
  
  CONCLUSIONS
  Using PCR amplification products to prepare the DNA microarray is quick, simple and effective.
authors.affiliation: Institute of Molecular Oncology, Guangzhou General Hospital of Guangzhou Command, Guangzhou 510010, China.
authors.forename: Zhao-hui
authors.initials: ZH
authors.lastname: Sun
authors.forename: Wen-ling
authors.initials: WL
authors.lastname: Zheng
authors.forename: Xiang-ming
authors.initials: XM
authors.lastname: Mao
authors.forename: Bao
authors.initials: B
authors.lastname: Zhang
authors.forename: Liang
authors.initials: L
authors.lastname: Lü
authors.forename: Xiao-dong
authors.initials: XD
authors.lastname: Ma
authors.forename: Rong
authors.initials: R
authors.lastname: Shi
authors.forename: Wen-li
authors.initials: WL
authors.lastname: Ma
mesh_terms: D006515:Hepatitis B virus / Q000235:genetics / Q000302:isolation & purification*; D003698:Hepatitis Delta Virus / Q000235:genetics / Q000302:isolation & purification*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*