title: Unnatural amino acid mutagenesis in mapping ion channel function.
abstract: Unnatural amino acid mutagenesis makes possible the site-specific incorporation of synthetic amino acids, enabling detailed structure-function studies as well as the incorporation of biophysical probes. This method has been adapted for use with heterologous expression in Xenopus oocytes, allowing experiments on ion channels.
authors.affiliation: Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA.
authors.forename: Darren L
authors.initials: DL
authors.lastname: Beene
authors.forename: Dennis A
authors.initials: DA
authors.lastname: Dougherty
authors.forename: Henry A
authors.initials: HA
authors.lastname: Lester
mesh_terms: D000596:Amino Acids / Q000737:chemistry* / Q000502:physiology; D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D006801:Humans; D007473:Ion Channels / Q000737:chemistry* / Q000502:physiology; D016297:Mutagenesis, Site-Directed / Q000502:physiology*; D009865:Oocytes; D013329:Structure-Activity Relationship; D014981:Xenopus
chemical_list: D000596:Amino Acids; D007473:Ion Channels