Mu-opioid receptor desensitization: role of receptor phosphorylation, internalization, and representation.
It is generally accepted that the internalization and desensitization of mu-opioid receptor (MOR) involves receptor phosphorylation and beta-arrestin recruitment. However, a mutant MOR, which is truncated after the amino acid residue Ser363 (MOR363D), was found to undergo phosphorylation-independent internalization and desensitization. As expected, MOR363D, missing the putative agonist-induced phosphorylation sites, did not exhibit detectable agonist-induced phosphorylation. MOR363D underwent slower internalization as reflected in the attenuation of membrane translocation of beta-arrestin 2 when compared with wild type MOR, but the level of receptor being internalized was similar to that of wild type MOR after 4 h of etorphine treatment. Furthermore, MOR363D was observed to desensitize faster than that of wild type MOR upon agonist activation. Surface biotinylation assay demonstrated that the wild type receptors recycled back to membrane after agonist-induced internalization, which contributed to the receptor resensitization and thus partially reversed the receptor desensitization. On the contrary, MOR363D did not recycle after internalization. Hence, MOR desensitization is controlled by the receptor internalization and the recycling of internalized receptor to cell surface in an active state. Taken together, our data indicated that receptor phosphorylation is not absolutely required in the internalization, but receptor phosphorylation and subsequent beta-arrestin recruitment play important roles in the resensitization of internalized receptors.
Qiu
Yu
Y
Department of Pharmacology, Medical School, University of Minnesota, Minneapolis, Minnesota 55455, USA. qiuxx@umn.edu
Law
Ping-Yee
PY
Loh
Horace H
HH
D000701:Analgesics, Opioid / Q000494:pharmacology; D019390:Arrestins / Q000378:metabolism; D001665:Binding Sites; D019921:Biotinylation; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002469:Cell Separation; D000242:Cyclic AMP / Q000378:metabolism; D000939:Epitopes / Q000737:chemistry; D005048:Etorphine / Q000494:pharmacology; D005434:Flow Cytometry; D006801:Humans; D007700:Kinetics; D018613:Microscopy, Confocal; D009154:Mutation; D010766:Phosphorylation; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D017450:Receptors, Opioid, mu / Q000378:metabolism*; D013997:Time Factors; D000071560:beta-Arrestin 2; D000071557:beta-Arrestins
C000605254:ARRB2 protein, human; D000701:Analgesics, Opioid; D019390:Arrestins; D000939:Epitopes; D017450:Receptors, Opioid, mu; D000071560:beta-Arrestin 2; D000071557:beta-Arrestins; D005048:Etorphine; D000242:Cyclic AMP