title: The influences of extremely low frequency magnetic fields on drug-induced convulsion in mouse.
abstract: This study investigated the effects of extremely low frequency magnetic fields (ELF-MFs) on the sensitivity of seizure response to bicuculline, picrotoxin and NMDA in mice. The mice were exposed to either a sham or 20 G ELF-MFs for 24 hours. Convulsants were then administered i.p. at various doses. The seizure induction time and duration were measured and lethal dose (LD50) and convulsant dose (CD50) of the clonic and tonic convulsion were calculated. The analysis of glutamate, glycine, taurine and GABA of mouse brain was accomplished by HPLC. The mice exposed to ELF-MFs showed moderately higher CD50, LD50 and onset time on the bicuculline-induced seizure. However, the ELF-MFs did not influence them in the NMDA and picrotoxin-induced seizures. After the exposure to MFs exposure, the glutamate level was increased and GABA was decreased significantly in NMDA and picrotoxin-induced seizure. The level of glutamate and GABA were not changed by MFs in bicuculline-induced seizure. These results suggest that ELF-MFs may alter the convulsion susceptibility through GABAergic mechanism with the involvement of the level of glutamate and GABA.
authors.affiliation: Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.
authors.forename: Ji Hyun
authors.initials: JH
authors.lastname: Sung
authors.forename: Ji Hoon
authors.initials: JH
authors.lastname: Jeong
authors.forename: Jeong Soo
authors.initials: JS
authors.lastname: Kim
authors.forename: Tai Sik
authors.initials: TS
authors.lastname: Choi
authors.forename: Joon Hong
authors.initials: JH
authors.lastname: Park
authors.forename: Hoe Yun
authors.initials: HY
authors.lastname: Kang
authors.forename: Young Sil
authors.initials: YS
authors.lastname: Kim
authors.forename: Dong Suk
authors.initials: DS
authors.lastname: Kim
authors.forename: Uy Dong
authors.initials: UD
authors.lastname: Sohn
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D003292:Convulsants / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004574:Electromagnetic Fields / Q000009:adverse effects; D051379:Mice; D008813:Mice, Inbred ICR; D012640:Seizures / Q000139:chemically induced* / Q000378:metabolism
chemical_list: D003292:Convulsants