[Nanobacteria in serum, bile and gallbladder mucosa of cholecystolithiasis patients].
OBJECTIVE
To find the distribution of nanobacteria in the serum, bile and gallbladder mucosa of cholecystolithiasis patients.


METHODS
The infection rate of nanobacteria was identified by ELISA in the serum samples from 338 healthy people and 76 patients with cholecystolithiasis (chi(2) = 0.89, P > 0.05). Nanobacteria were cultured from the bile samples in 57 patients with cholecystolithiasis and 18 non-cholelithiasis patients and identified by immunohistochemical staining and TEM (chi(2) = 29.80, P < 0.05). Forty samples of gallbladder mucosa randomly selected from the 57 cholecystolithiasis patients were identified by immunohistochemical staining and compared with the corresponding bile samples.


RESULTS
The infection rate of nanobacteria was 8.0% and 31.6% for the serum samples of the healthy people and cholecystolithiasis patients, respectively. The positive rate of nanobacteria in the bile samples was 61.3% and there was no significant difference in the bile of the cholecystolithiasis patients and the control group (61.4% vs. 61.1%). Fourteen positive patients had infection of nanobacteria in the gallbladder mucosa, submucosa, and calcific field.


CONCLUSIONS
The infection rate of nanobacteria was 8% in the serum samples from the healthy people. There are nanobacteria in the serum, bile, and gallbladder mucosa. The infection of the nanobacteria may result in calcification and fibrosis of the gallbladder.
Wen
Yu
Y
Hepatobiliary Diseases Laboratory, Second Xiangya Hospital, Central-South University, Changsha 410011, China.
Li
Yong-guo
YG
Yang
Zhu-lin
ZL
Wang
Xue-jun
XJ
Wei
Hong
H
Liu
Wei
W
Tan
Ai-ling
AL
Miao
Xiong-ying
XY
Wang
Qun-wei
QW
Huang
Sheng-fu
SF
Kajander
E Olavi
EO
Ciftcioglu
Neva
N
D000328:Adult; D001419:Bacteria / Q000302:isolation & purification* / Q000648:ultrastructure; D001646:Bile / Q000382:microbiology*; D041761:Cholecystolithiasis / Q000097:blood* / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005704:Gallbladder / Q000382:microbiology*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D046529:Microscopy, Electron, Transmission; D008875:Middle Aged; D009092:Mucous Membrane / Q000382:microbiology; D055815:Young Adult