Monitoring bone loss using ELISA and surface plasmon resonance (SPR) technology.
Osteoporosis influences the health of the females who are in menopause phase. The techniques to detect the markers of bone turnover is very important for preventing osteoporosis. ELISA was developed for detection of urinary N-telopeptide (NTx) as an osteoporosis marker. Our aim is to develop a sensitive method to detect NTx excretion using surface plasmon resonance (SPR). Samples collected were assayed and results suggest that our SPR-based method is promising for monitoring bone loss.
Lung
Feng-Di T
FD
Department of Nutrition, China Medical College, Taichung, Taiwan. fdlung@mail.cmc.edu.tw
Chen
Huey-Yi
HY
Lin
Hsing-Tzu
HT
D015415:Biomarkers / Q000032:analysis*; D001862:Bone Resorption / Q000378:metabolism*; D003094:Collagen / Q000652:urine*; D024042:Collagen Type I; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D015663:Osteoporosis, Postmenopausal / Q000378:metabolism*; D010455:Peptides / Q000652:urine*; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance
D015415:Biomarkers; D024042:Collagen Type I; D010455:Peptides; C052929:collagen type I trimeric cross-linked peptide; D003094:Collagen