title: Equilibrium protein folding-unfolding process involving multiple intermediates.
abstract: Mathematical models for the protein folding-unfolding process involving multiple intermediates have been derived. Computer fitting of the experimental data to this model generates various thermodynamic parameters for the folding-unfolding process. In this way, the complex folding-unfolding process of the multi-domain proteins can be analysed in a quantitative way. The application of the folding-unfolding model involving seven stages in human placental alkaline phosphatase is described.
authors.affiliation: Department of Life Science, National Chung-Hsing University, Taichung, Taiwan.
authors.forename: Hui-Chih
authors.initials: HC
authors.lastname: Hung
authors.forename: Yu-Hou
authors.initials: YH
authors.lastname: Chen
authors.forename: Guang-Yaw
authors.initials: GY
authors.lastname: Liu
authors.forename: Hwei-Jen
authors.initials: HJ
authors.lastname: Lee
authors.forename: Gu-Gang
authors.initials: GG
authors.lastname: Chang
mesh_terms: D000469:Alkaline Phosphatase / Q000737:chemistry*; D003198:Computer Simulation; D005260:Female; D006801:Humans; D007700:Kinetics; D008956:Models, Chemical; D010920:Placenta / Q000201:enzymology; D017510:Protein Folding
chemical_list: D000469:Alkaline Phosphatase