title: Efficiency of a cysteine-taurine-threonine-serine supplemented parenteral nutrition in an experimental model of acute inflammation.
abstract: OBJECTIVE
  As is the case with glutamine, requirements for amino acids such as cysteine, taurine, and serine may be increased in stress situations. This study evaluated the potential usefulness of supplementation of total parenteral nutrition with a cysteine, taurine, threonine, and serine mixture (SEAS), with or without glutamine, in an experimental model of turpentine-induced acute inflammation.
  
  
  METHODS
  Prospective, controlled animal study in male Sprague-Dawley rats.
  
  
  METHODS
  Twenty-seven rats received isonitrogenous, isocaloric total parenteral nutrition (260 kcal/kg, 2 gN/kg per day) for 5 days. They were divided into three groups according to the composition of the amino acid admixture: standard amino acids (control, n=9), standard amino acids partly substituted with SEAS (n=10) or with SEAS and glutamine (n=8). All rats received two subcutaneous turpentine injections (0.5 ml/100 g) 24 h (day 2) and 72 h (day 4) after the initiation of parenteral nutrition and were killed on day 5.
  
  
  RESULTS
  Nitrogen balance was significantly increased (control 53+/-29, SEAS 153+/-21, SEAS+Gln 187+/-32 mg/24 h) and urinary 3-methylhistidine/creatinine ratio decreased (control 55+/-4, SEAS 43+/-4, SEAS+Gln 38+/-3 micro mol/mmol) on day 5 in the two SEAS-treated groups. Hepatic and extensor digitorum longus muscle protein contents were significantly higher in the SEAS+Gln-treated group than in the other two groups. In addition to slight differences in liver amino acid content, other parameters including liver glutathione did not differ significantly between groups.
  
  
  CONCLUSIONS
  Improved nitrogen balance and reduction in urinary 3-methylhistidine suggest that SEAS supplementation improves nitrogen homeostasis in an experimental model of acute inflammation. Glutamine addition further improves protein status.
authors.affiliation: Laboratoire de Biologie de la Nutrition, Faculté de Pharmacie, Université Paris 5, 75270 Paris 06, France.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Osowska
authors.forename: Jean-Pascal
authors.initials: JP
authors.lastname: De Bandt
authors.forename: Samira
authors.initials: S
authors.lastname: Chaïb
authors.forename: Nathalie
authors.initials: N
authors.lastname: Neveux
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Bérard
authors.forename: Luc
authors.initials: L
authors.lastname: Cynober
mesh_terms: D000208:Acute Disease; D000818:Animals; D003545:Cysteine / Q000008:administration & dosage*; D019587:Dietary Supplements; D007249:Inflammation / Q000178:diet therapy*; D008297:Male; D010288:Parenteral Nutrition; D011446:Prospective Studies; D051381:Rats; D017207:Rats, Sprague-Dawley; D012694:Serine / Q000008:administration & dosage*; D013654:Taurine / Q000008:administration & dosage*; D013912:Threonine / Q000008:administration & dosage*
chemical_list: D013654:Taurine; D013912:Threonine; D012694:Serine; D003545:Cysteine