A potentially important excretory-secretory product of Giardia lamblia.
In this study we have reported the detailed characterization of a 58 kDa excretory-secretory product (ESP) of Giardia lamblia. The method of purification has been simplified which has improved the purification fold as well as the yield of the ESP. The binding efficacy of disialoganglioside (GD2) to the purified ESP was found to be maximum among all other gangliosides used. The N-terminal sequence of the immunoreactive 29 kDa peptide obtained from partial tryptic digest of the ESP was found to be AD-FVPQVST. The IgG against the purified ESP (IgGES) showed cross-reactivity with the binding subunit of the commercially available cholera toxin and also with two protein bands of western cottonmouth moccasin snake toxin. The ESP could accumulate fluid in the intestine of sealed adult mice and also induce morphological changes in HEp-2 cells. The crude extract of G. lamblia trophozoites preincubated with Escherichia coli revealed 8-fold augmentation in the cytopathic activity on HEp-2 cells as compared to that of crude preparation from trophozoites only.
Shant
Jasleen
J
Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh 160012, India.
Bhattacharyya
Shalmoli
S
Ghosh
Sujata
S
Ganguly
Nirmal Kumar
NK
Majumdar
Siddhartha
S
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002850:Chromatography, Gel; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D005732:Gangliosides / Q000378:metabolism; D016829:Giardia lamblia / Q000378:metabolism*; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000633:toxicity; D007074:Immunoglobulin G / Q000276:immunology; D007421:Intestine, Small / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D015800:Protozoan Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000633:toxicity; D011817:Rabbits; D014118:Toxins, Biological / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000633:toxicity
D005732:Gangliosides; D006023:Glycoproteins; D007074:Immunoglobulin G; D015800:Protozoan Proteins; D014118:Toxins, Biological