In vitro effects of flavone from leaves of Diospyros kaki on rat cardiac myocyte apoptosis induced by hypoxia-reoxygenation and advanced glycation end products.
OBJECTIVE
To investigate the effects of flavone extracted from the leaves of Diospyros kaki on the apoptosis of rat cardiac myocytes induced by hypoxia-reoxygenation and advanced glycation end products in vitro.


METHODS
The cardiac myocytes were isolated from neonatal SD rats and cultured in vitro for 72 h. Apoptosis of the cultured cells was induced by hypoxia-reoxygenation and advanced glycation end products (AGEs) respectively, and the effects of flavone extracted from the leaves of Diospyros kaki on the cell apoptosis was observed by measuring the apoptotic rates using flow cytometry.


RESULTS
Apoptosis of neonatal rat cardiac myocytes was induced by hypoxia-reoxygenation in a time-dependent manner (6.05% +/-0.46% vs 12.45% +/-1.66%, P< 0.05), and flavone treatment of the cells significantly lowered the apoptotic rates. AGEs alone also induced apoptosis of neonatal rat cardiac myocytes (11.03+/-0.39 vs 6.25+/-0.48, P< 0.05), which was inhibited by flavone (8.40+/-0.47 vs 11.03+/-0.39, P < 0.05).


CONCLUSIONS
Flavone extracted from the leaves of Diospyros kaki can inhibit the apoptosis of in vitro cultured neonatal rat cardiac myocytes induced by hypoxia-reoxygenation and advanced glycation end products.
Ouyang
Ping
P
Department of Cardiology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. ouyp@msn.com
Bei
Wei-jJian
WJ
Lai
Wen-yan
WY
Xu
Ding-li
DL
Peng
Wen-lie
WL
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015687:Cell Hypoxia; D029788:Diospyros / Q000737:chemistry*; D005260:Female; D005419:Flavonoids / Q000494:pharmacology*; D017127:Glycation End Products, Advanced / Q000633:toxicity*; D008297:Male; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000473:pathology; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley
D005419:Flavonoids; D017127:Glycation End Products, Advanced