title: [Evaluation on reducing effect of eye drop on herpes simplex virus-I in vitro].
abstract: OBJECTIVE
  To observe the effect of herbal eye drop Reduqing (clearing toxic heat) on herpes simplex virus (HSV) on the cytopathic effect in cell culture in vitro.
  
  
  METHODS
  The maximal non-toxic Reduqing eye drop concentration added to cell culture infected with 100 and 10TCID50 of herpes simplex virus? (HSV-1) to observe the inhibitory effect of the medicine on HSV-I induced cytopathic effect.
  
  
  RESULTS
  The Reduqing eye drop at maximal non-toxic concentration could obviously inhibit the cytopatic effect induced by 100TCID50 and 10TCID50 HSV-I. No statistically significant difference was found when compared with 0.1% acyclovir eye drop (P>0.05), on cytopathic effect occurred with boty eye drops. However, there was significant difference when compared with control group(P<0.05). The inhibitory effect of concentrations of Reduqing at 500,000 and 63,000 microg/L was stronger than that of 2,000,000 and 1,000,000 microg/L (P 0.01). The maximal tolerable concentration of Reduqing eye drop by the cell was 2,000,000 microg/L (1:80 dilution), which was higher than that of 5,000 microg/L(1:400 dilution) acyclovir eye drop.
  
  
  CONCLUSIONS
  These results suggest that Reduqing eye drop could markedly inhibit the cytopathic effects caused by HSV-I. The Reduqing eye drop showed remarkably low toxic effect to the cells as compared to acyclovir eye drop.
authors.affiliation: Department of Ophthalmology, Hangzhou Hospital of Traditional Chinese Medicine, Hangzhou 310007, China.
authors.forename: Liang-yu
authors.initials: LY
authors.lastname: Zhong
authors.forename: Jing-yi
authors.initials: JY
authors.lastname: Chen
authors.forename: Hong-mei
authors.initials: HM
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Xiao-meng
authors.initials: XM
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Lai
authors.forename: Ya-qin
authors.initials: YQ
authors.lastname: Liu
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D018259:Herpesvirus 1, Human / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D009883:Ophthalmic Solutions
chemical_list: D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; D009883:Ophthalmic Solutions