title: Estrous cyclicity and ovarian follicles in female rats after prenatal exposure to 3,3',4,4',5-pentachlorobiphenyl.
abstract: Alterations of estrous cyclicity and ovarian follicles following prenatal exposure to PCB126 were examined. Female SD rats were given (i.g.) 25 pg, 2.5, 250 ng and 7.5 microg of PCB126/kg or the vehicle on days 13-19 postconception. Vaginal opening (VO) in the 250 ng and 7.5 microg offspring was significantly delayed. All groups showed irregular estrous cyclicity following VO, but it became normal after a few days. However, the start of normal estrous cyclicity following VO in the 2.5, 250 ng and 7.5 microg groups was significantly delayed. At 30 and 50 days old, the 2.5, 250 ng and 7.5 microg groups showed significantly fewer antral follicles and a higher number of atretic follicles. The 7.5 microg group at 50 days old revealed significantly fewer corpus luteums. In 50-day-old offspring, the 2.5, 250 ng and 7.5 microg groups showed a significant reduction in serum 17beta-estradiol and progesterone levels and significantly higher levels of PCB126 in the fatty tissue compared with the vehicle group. Thus, while the prenatal dose of PCB126 used in this study did not induce malformation of the external genitalia or persistent ovarian disruption, disruption of ovarian function at puberty was found in the 2.5 ng group of pups born to dams exposed to 17.5 ng/kg PCB126. The present study suggests that PCB126, at least in part, exerted direct effects on the ovary as shown by the disruption of estrous cyclicity.
authors.affiliation: Department of Pathology, Kyorin University School of Medicine, Mitaka, Tokyo, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Muto
authors.forename: Noboru
authors.initials: N
authors.lastname: Imano
authors.forename: Kenji
authors.initials: K
authors.lastname: Nakaaki
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Takahashi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hano
authors.forename: Shin
authors.initials: S
authors.lastname: Wakui
authors.forename: Masakuni
authors.initials: M
authors.lastname: Furusato
mesh_terms: D000818:Animals; D004958:Estradiol / Q000097:blood; D004965:Estrogen Antagonists / Q000493:pharmacokinetics / Q000633:toxicity*; D030762:Estrous Cycle / Q000187:drug effects*; D005260:Female; D006651:Histocytochemistry; D008297:Male; D006080:Ovarian Follicle / Q000187:drug effects* / Q000378:metabolism; D011078:Polychlorinated Biphenyls / Q000493:pharmacokinetics / Q000633:toxicity*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D011374:Progesterone / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D012741:Sexual Maturation / Q000187:drug effects*
chemical_list: D004965:Estrogen Antagonists; D011374:Progesterone; D004958:Estradiol; D011078:Polychlorinated Biphenyls; C023035:3,4,5,3',4'-pentachlorobiphenyl