[Effect of T3 on the expression of transferrin receptor and ferritin in K562 cells and its possible mechanism].
OBJECTIVE
To explore the effect of T(3) on the expression of transferrin receptor (TfR) and ferritin (Fn) in K562 cells and its possible mechanism.


METHODS
Flow cytometry was used for the detection of TfR expression, radioimmunoassay for Fn expression, RNA/protein band shift assay for the binding activity of iron regulatory protein (IRP) and iron responsive elements (IRE), and RT-PCR for TfR and Fn mRNA levels.


RESULTS
Different concentration of T(3) significantly increased Fn expression of K562 cells, especially at 100 nmol/L and 200 nmol/L (p < 0.05). However, T(3) had no effect on TfR expression. T(3) decreased the binding activity between IRP and IRE, particularly at concentration of 50 nmol/L. Different concentration of T(3) increased Fn-H mRNA level at different time point while it had no effect on TfR mRNA level.


CONCLUSIONS
T(3) increased Fn expression of K562 cells through the possible mechanisms of either the post-transcriptional regulation or transcriptional modulation.
Zhou
Mi
M
Pediatric Department, Second Huaxi Hospital, Sichuan University, Chengdu 610041, China.
Liao
Qing-kui
QK
Li
Feng-yi
FY
Li
Qiang
Q
Luo
Chun-hua
CH
Gao
Ju
J
Jia
Cang-song
CS
Yang
Chong-li
CL
D005293:Ferritins / Q000096:biosynthesis* / Q000187:drug effects / Q000235:genetics; D005434:Flow Cytometry; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects; D006801:Humans; D020014:K562 Cells; D012333:RNA, Messenger / Q000235:genetics; D011863:Radioimmunoassay; D011990:Receptors, Transferrin / Q000096:biosynthesis* / Q000187:drug effects / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014284:Triiodothyronine / Q000494:pharmacology*
D012333:RNA, Messenger; D011990:Receptors, Transferrin; D014284:Triiodothyronine; D005293:Ferritins