Effects of antibiotics that inhibit the bacterial peptidoglycan synthesis pathway on moss chloroplast division.
Moss chloroplasts should prove useful for studying the cyanobacteria-derived system in chloroplasts. To determine the effects of antibiotics that inhibit bacterial peptidoglycan synthesis, the numbers of chloroplasts in treated Physcomitrella patens cells were counted. Ampicillin and D-cycloserine caused a rapid decrease in the number of chloroplasts per cell. Fosfomycin affected half of the cells, while vancomycin affected a few cells. Conversely, bacitracin had no effect. With the decrease in chloroplast number, macrochloroplasts appeared in antibiotic-treated cells. Removal of the antibiotics resulted in the recovery of chloroplast number, suggesting that the decrease in number was directly dependent on the antibiotic treatment. Microscopic observations showed that the decrease in the number of chloroplasts resulted from cell division without chloroplast division. These results suggest that enzymes derived from the bacterial peptidoglycan synthesis pathway are related to moss chloroplast division.
Katayama
Nami
N
Graduate School of Science and Technology, Kumamoto University, Kumamoto, 860-8555 Japan.
Takano
Hiroyoshi
H
Sugiyama
Motoji
M
Takio
Susumu
S
Sakai
Atsushi
A
Tanaka
Kan
K
Kuroiwa
Haruko
H
Ono
Kanji
K
D000667:Ampicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001414:Bacitracin / Q000494:pharmacology; D019068:Bryopsida / Q000187:drug effects* / Q000378:metabolism; D002736:Chloroplasts / Q000187:drug effects* / Q000502:physiology; D003523:Cycloserine / Q000494:pharmacology; D005578:Fosfomycin / Q000494:pharmacology; D010457:Peptidoglycan / Q000096:biosynthesis*; D014640:Vancomycin / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D010457:Peptidoglycan; D001414:Bacitracin; D005578:Fosfomycin; D014640:Vancomycin; D000667:Ampicillin; D003523:Cycloserine