Ribosomal localization of translation initiation factor IF2.
Bacterial translation initiation factor IF2 is a GTP-binding protein that catalyzes binding of initiator fMet-tRNA in the ribosomal P site. The topographical localization of IF2 on the ribosomal subunits, a prerequisite for understanding the mechanism of initiation complex formation, has remained elusive. Here, we present a model for the positioning of IF2 in the 70S initiation complex as determined by cleavage of rRNA by the chemical nucleases Cu(II):1,10-orthophenanthroline and Fe(II):EDTA tethered to cysteine residues introduced into IF2. Two specific amino acids in the GII domain of IF2 are in proximity to helices H3, H4, H17, and H18 of 16S rRNA. Furthermore, the junction of the C-1 and C-2 domains is in proximity to H89 and the thiostrepton region of 23S rRNA. The docking is further constrained by the requisite proximity of the C-2 domain with P-site-bound tRNA and by the conserved GI domain of the IF2 with the large subunit's factor-binding center. Comparison of our present findings with previous data further suggests that the IF2 orientation on the 30S subunit changes during the transition from the 30S to 70S initiation complex.
Marzi
Stefano
S
Laboratory of Genetics, Department of Biology MCA, University of Camerino, 62032 Camerino (MC) Italy.
Knight
William
W
Brandi
Letizia
L
Caserta
Enrico
E
Soboleva
Natalia
N
Hill
Walter E
WE
Gualerzi
Claudio O
CO
Lodmell
J Stephen
JS
D001483:Base Sequence; D003198:Computer Simulation; D003545:Cysteine / Q000737:chemistry; D001411:Geobacillus stearothermophilus / Q000378:metabolism; D006868:Hydrolysis; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D039881:Prokaryotic Initiation Factor-2 / Q000737:chemistry / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000737:chemistry / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*
D039881:Prokaryotic Initiation Factor-2; D012335:RNA, Ribosomal; D003545:Cysteine