Effect of P-selectin monoclonal antibody on metastasis of gastric cancer and immune function.
OBJECTIVE
To investigate the effect of cell adhesion molecule P-selectin monoclonal antibody (Mab) on metastasis and immune function of mice orthototopically implanted with human gastric cancer tissue.


METHODS
SCID mice were implanted orthotopically with SGC-7901 human gastric carcinoma tissue. Starting from day 3 after operation, animals were given intravenously PBS or P-selectin Mab (100 microg/injection) (for both normal mice and tumor-implanted mice with tumors), twice weekly for 3 weeks. Two animals in each group were sacrificed randomly at the 1st, 2nd, 4th week and 6th week. While T cell and B cell transformation indices were determined with the (3)H TdR infiltration method, the NK cell activity was detected by the LDH release method.


RESULTS
The metastatic rate in the P-selectin Mab treated group was lower than that in the PBS treated group (with tumors). The NK activity of normal mice increased over time. The immune functions (T, B cell function, NK activity) of the tumor group in the 6th week were significantly lower than those in the 4th week, but the change was attenuated by P-selectin Mab.


CONCLUSIONS
P-selectin Mab could suppress the metastasis of gastric cancer with no adverse effect on host immune function.
Chen
Jin-Lian
JL
Department of Gastroenterology, Shanghai Jiao-Tong University affiliated Sixth People's Hospital, Shanghai 200233, China. wqq_021002@163.com
Chen
Wei-Xiong
WX
Zhu
Jin-Shui
JS
Chen
Ni-Wei
NW
Zhou
Tong
T
Yao
Ming
M
Zhang
Dong-Qing
DQ
Wu
Yun-Lin
YL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000276:immunology; D004195:Disease Models, Animal; D007167:Immunotherapy; D007694:Killer Cells, Natural / Q000276:immunology; D008297:Male; D051379:Mice; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D019007:P-Selectin / Q000276:immunology*; D013274:Stomach Neoplasms / Q000276:immunology / Q000556:secondary* / Q000628:therapy*; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D019007:P-Selectin