Simultaneous determination of residual antiparasitic lactones in bovine muscle and liver by liquid chromatography with fluorescence detection.
Abamectin, doramectin, eprinomectin, ivermectin, milbemectin, and moxidectin in bovine muscle and liver were extracted with acetonitrile. The extracts were partitioned with n-hexane and then evaporated to dryness. The residue was cleaned up on Bond Elute NH2 cartridge, and the drugs were eluted from the cartridge with methanol-ethyl acetate (3 + 7). The eluate was evaporated to dryness, and residues were derivatized with N,N-dimethylformamide-acetic anhyride-1-methylimidazole. The derivatives were determined by liquid chromatography with fluorescence detection. Recoveries of the 6 drugs were 79.6-63.8% in muscle and 71.6-60.6% in liver at 0.01 ppm levels. The quantitation limits were 5 ppb for each drug.
Nagata
Tomoko
T
The Public Health Laboratory of Chiba Prefecture, 666-2, Nitona-cho, Chuo-ku, Chiba City 260-7815, Japan. t.ngt2@mc.pref.chiba.jp
Miyamoto
Fumio
F
Hasegawa
Yasuyuki
Y
Ashizawa
Eiichi
E
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis; D000977:Antiparasitic Agents / Q000032:analysis*; D002417:Cattle; D002853:Chromatography, Liquid; D004350:Drug Residues / Q000032:analysis*; D005453:Fluorescence; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000032:analysis; D007783:Lactones / Q000032:analysis*; D008099:Liver / Q000737:chemistry*; D018942:Macrolides; D009132:Muscles / Q000737:chemistry*
D000900:Anti-Bacterial Agents; D000977:Antiparasitic Agents; D007783:Lactones; D018942:Macrolides; C048324:abamectin; D007559:Ivermectin; C101434:eprinomectin; C084101:doramectin; C027837:moxidectin