Cellular biosensing system for assessing immunomodulating effects on the inducible nitric oxide synthase (iNOS) cascade.
A cellular biosensing system has been constructed to assess the biological safety/toxicity of chemicals. Detection of nitric oxide (NO) by the cellular biosensing system was used as a readout for assessing the immunomodulating effects of various chemicals, because some are known to induce NO synthase (iNOS) activity thereby increasing NO production. The macrophage-like cell line, RAW264.7, was cultured on the electrode coated with a polyion complex layer. The potent immune activating abilities of lipopolysaccharide could be verified by the cellular biosensing system: NO release from cells was detected within 600 ms by double potential step chronoamperometry.
Kamei
Ken-ichiro
K
Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259, Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.
Haruyama
Tetsuya
T
Mie
Masayasu
M
Yanagida
Yasuko
Y
Kobatake
Eiry
E
Aizawa
Masuo
M
D000276:Adjuvants, Immunologic / Q000032:analysis; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D002478:Cells, Cultured; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II; D009609:Nitrous Oxide / Q000378:metabolism; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D018675:Toxicity Tests / Q000295:instrumentation* / Q000379:methods
D000276:Adjuvants, Immunologic; D008070:Lipopolysaccharides; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; D009609:Nitrous Oxide