[Transfection of chk1/2 antisense oligonucleotide to HL-60 cell line increases the apoptotic sensitivity to irradiation].
OBJECTIVE
To block signal transduction of cell cycle checkpoints by antisense blocking of chk1/2 gene to increase the radiation sensitivity of HL-60 cell line.


METHODS
To transfect the HL-60 cell with chk1/2 antisense and sense chain alone and in combination, expose the cells to irradiation at 24 h after the transfection, the chk1 protein change was assayed by Western blot and the cell cycles and annexin V apoptosis rates by FCM.


RESULTS
The irradiated apoptosis sensitivity was increased by antisense blocking of chk1 gene in HL-60 cell line, the apoptotic rate was 26.31% being significantly higher than that of the sense blocking (10.34%) (P < 0.05), Furthermore, the G(2)/M phase blocking phenomenon decreased and a synergic effect was observed in antisense blocking both the chk1 and chk2 genes.


CONCLUSIONS
Antisense blocking of chk1/chk2 could increase the apoptotic sensitivity to irradiation.
Tang
Yi
Y
Hematology Department of Tongji Hospital, Tongji College of Huazhong Science & Technology University, Wuhan 430030, China.
Liu
Wen-li
WL
Zhou
Jian-feng
JF
Wan
Li-ping
LP
Gao
Qing-lei
QL
Wu
Jian-hong
JH
D017209:Apoptosis / Q000235:genetics / Q000528:radiation effects; D002453:Cell Cycle / Q000528:radiation effects; D000071877:Checkpoint Kinase 1; D064447:Checkpoint Kinase 2; D018922:HL-60 Cells; D006801:Humans; D016376:Oligonucleotides, Antisense / Q000235:genetics*; D011494:Protein Kinases / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D011836:Radiation Tolerance; D014162:Transfection
D016376:Oligonucleotides, Antisense; D011494:Protein Kinases; D064447:Checkpoint Kinase 2; C000605867:CHEK1 protein, human; C578634:CHEK2 protein, human; D000071877:Checkpoint Kinase 1; D017346:Protein Serine-Threonine Kinases