High expression of a human lactoferrin in transgenic tobacco cell cultures.
Transgenic Nicotiana tabacum cell lines were developed expressing the human lactoferrin gene driven by the oxidative stress-inducible peroxidase (SWPA2) promoter. Western blot analysis showed the accumulation of both the full-length human lactoferrin protein as well as a immuno-reactive truncated fragment. Accumulation of human lactoferrin as monitored by ELISA increased proportionally to cell growth and reached a maximal (up to 4.3% of total soluble proteins) at the stationary phase of growth. Protein extracts from transgenic tobacco cells exhibited antibacterial activity.
Choi
Sun-Mee
SM
Laboratory of Plant Cell Biotechnology, Korea Research Institute of Bioscience and Biotechnology, Oun-Dong 52, Yusong, Daejeon 305-806, Korea.
Lee
Ok-Sun
OS
Kwon
Suk-Yoon
SY
Kwak
Sang-Soo
SS
Yu
Dae-Yeul
DY
Lee
Haeng-Soon
HS
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002478:Cells, Cultured; D004926:Escherichia coli / Q000187:drug effects; D005786:Gene Expression Regulation; D006801:Humans; D007781:Lactoferrin / Q000096:biosynthesis* / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D011786:Quality Control; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D012486:Salmonella typhimurium / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D011994:Recombinant Proteins; D007781:Lactoferrin