title: Mouse ICSI with frozen-thawed sperm: the impact of sperm freezing procedure and sperm donor strain.
abstract: Normal mouse offspring can be obtained from oocytes injected with frozen-thawed spermatozoa without cryoprotection, however, embryo development can be affected by sperm freezing procedure and sperm donor strain. In this study we observed that direct contact of mouse spermatozoa with liquid nitrogen did not affect their ability to activate injected oocytes but severely restricted subsequent in vitro embryo development to blastocyst stage. Tris-EDTA buffer and M2 were also shown to be better sperm freezing extenders than DPBS, allowing higher developmental potential. In addition, differences in embryo development obtained by intracytoplasmic sperm injection (ICSI) with frozen-thawed spermatozoa were observed between hybrid sperm donor strains. Frozen-thawed B6D2F1 spermatozoa provided higher embryo development than sperm cells from C57CBAF1.
authors.affiliation: Dpto. de Reproducción Animal y Conservación de Recursos Zoogeneéticos, INIA, Ctra.de La Coruña, Madrid, Spain. pmoreira@inia.es
authors.forename: Pedro N
authors.initials: PN
authors.lastname: Moreira
authors.forename: Adela
authors.initials: A
authors.lastname: Jimenéz
authors.forename: Raul
authors.initials: R
authors.lastname: Fernández
authors.forename: Ninoska
authors.initials: N
authors.lastname: Bury-Madrid
authors.forename: Julio
authors.initials: J
authors.lastname: De la Fuente
authors.forename: Belen
authors.initials: B
authors.lastname: Pintado
authors.forename: Alfonso
authors.initials: A
authors.lastname: Gutiérrez-Adán
mesh_terms: D000818:Animals; D015925:Cryopreservation; D005260:Female; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa