Functional expression of recombinant tumstatin in stably transformed Drosophila melanogaster S2 cells.
Recombinant tumstatin was expressed in stably transformed Drosophila melanogaster S2 cells and secreted into the medium with a molecular size of 29 kDa. Recombinant endostatin was also purified to homogeneity using a simple one-step Ni2+ affinity fractionation. Purified recombinant tumstatin inhibited endothelial cell proliferation in a dose-dependent manner. The concentration at half-maximum inhibition for recombinant tumstatin was approx. 0.7 microg ml(-1). A maximum production of 4.6 microg recombinant tumstatin (10(7) cells)(-1) was obtained in a T-flask culture of S2 cells, 7 d after induction with 0.5 mM CuSO4.
Jeon
Hee Kyoung
HK
Department of Genetic Engineering and Plant Metabolism Research Center, Kyung Hee University, Suwon 449-701, Korea.
Chang
Kyung Hwa
KH
Kim
Kyung Il
KI
Chung
In Sik
IS
D000818:Animals; D001324:Autoantigens / Q000096:biosynthesis* / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology; D002417:Cattle; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D024141:Collagen Type IV / Q000096:biosynthesis* / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology; D004331:Drosophila melanogaster / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects; D005786:Gene Expression Regulation; D006801:Humans; D016297:Mutagenesis, Site-Directed; D011786:Quality Control; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology
D001324:Autoantigens; D024141:Collagen Type IV; D011994:Recombinant Proteins; C057112:type IV collagen alpha3 chain