Cetobacterium somerae sp. nov. from human feces and emended description of the genus Cetobacterium.
Phenorypic and phylogenetic studies were performed on four isolates of an unidentified gram-negative, microaerotolerant, non-spore-forming, rod-shaped bacterium isolated from the feces of children. The unknown organism was bile resistant and produced acetic acid as the major end product of metabolism of peptides and carbohydrates. It possessed a low DNA G + C content of 31 mol %. Comparative 16S rRNA gene sequencing demonstrated that the four isolates were phylogenetically identical (100% 16S rRNA sequence similarity) and represent a hitherto unknown sub-line within the genus Cetobacterium. The novel bacterium displayed approximately 5% sequence divergence with Cetobacterium ceti, and can be readily distinguished from the latter by physiological and biochemical criteria. Based on phylogenetic and phenotypic evidence, it is proposed that the unknown fecal bacterium be classified in the genus Cetobacterium, as Cetobacterium somerae sp. nov. The proposed type strain of Cetobacterium somerae is WAL 14325(T) (ATCC BAA-474(T) = CCUG 46254T).
Finegold
Sydney M
SM
Research Service, VA Medical Center West Los Angeles, CA 90073, USA. sidfinegol@aol.com
Vaisanen
Marja-Liisa
ML
Molitoris
Denise R
DR
Tomzynski
Thomas J
TJ
Song
Yuli
Y
Liu
Chengxu
C
Collins
Matthew D
MD
Lawson
Paul A
PA
D017668:Age of Onset; D001321:Autistic Disorder / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D050260:Carbohydrate Metabolism; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005243:Feces / Q000382:microbiology*; D016946:Gram-Negative Aerobic Rods and Cocci / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007422:Intestines / Q000382:microbiology; D008297:Male; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D021521:Ribotyping; D013045:Species Specificity; D014640:Vancomycin / Q000627:therapeutic use
D004269:DNA, Bacterial; D005227:Fatty Acids; D014640:Vancomycin