In vivo cardiovasculogenesis by direct injection of isolated adult mesenchymal stem cells.
The characterization of mesenchymal stem cells (MSCs) is of biological and clinical interest. We demonstrate that isolated MSCs, defined by CD34(low) c-kit(+) CD140a(+) Sca-1(high), are able to differentiate into cardiomyocytes, endothelial cells, and pericytes or smooth muscle cells by direct injection into adult heart. In skeletal muscle and lung, they also contributed to formation of the vasculature. MSCs did not transform into malignant cells or form excess extracellular matrix. This study suggests that MSCs may supply an ideal donor source of cardiovascular cells in patients with cardiopulmonary diseases.
Gojo
Satoshi
S
National Research Institute for Child Health and Development, Tokyo 157-8535, Japan.
Gojo
Noriko
N
Takeda
Yukiji
Y
Mori
Taisuke
T
Abe
Hitoshi
H
Kyo
Shunei
S
Hata
Jun-ichi
J
Umezawa
Akihiro
A
D000818:Animals; D001854:Bone Marrow Cells; D002319:Cardiovascular System / Q000166:cytology* / Q000254:growth & development; D004730:Endothelium, Vascular / Q000166:cytology; D005260:Female; D006085:Graft Survival; D049452:Green Fluorescent Proteins; D016130:Immunophenotyping; D008164:Luminescent Proteins / Q000235:genetics; D008648:Mesoderm / Q000166:cytology* / Q000637:transplantation; D051379:Mice; D008809:Mice, Inbred C3H; D018485:Muscle Fibers, Skeletal / Q000166:cytology; D009206:Myocardium / Q000166:cytology; D018919:Neovascularization, Physiologic; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism / Q000637:transplantation*; D033581:Stem Cell Transplantation / Q000379:methods*; D014161:Transduction, Genetic
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins