Immunohistochemical localization of inhibin and activin-like proteins in the brain, pituitary gland, and the ovary of thin-lipped grey mullet, Liza ramada (Risso).
Inhibin and activin, dimeric protein hormones originally isolated from mammalian gonads, are involved in the regulation of vertebrate reproduction. In the present study, we investigated the localization of inhibin and activin subunits (alpha, beta(A), and beta(B)) immunoreactivity in the brain, pituitary, and ovary of female Liza ramada using immunohistochemistry. We demonstrate that activin beta(A) subunit immunoreactivity is found in most of the brain regions: telencephalon (Tel), midbrain tegmentum (MT), and cerebellum (C). In the pituitary gland, activin beta(A) subunit immunoreactivity is observed in nerve fibers that are in close contact with somatotrophs and gonadotrophs. Strong immunostaining of activin beta(B) subunit is detected in melanotropin (MSH)-producing cells in pars intermedia (PI), which are in close contact with gonadotrophs. In the ovary, the cytoplasm as well as follicle cells of previtellogenic oocytes exhibited strong immunoreactivity of activin beta(A). However, the activin beta(A) and beta(B) immunoreactivity is decreased when the oocytes entered the vitellogenic stage (recrudescence). The progress in the accumulation of yolk within oocytoplasm is accompanied by a decrease in the activin beta(A) and beta(B) immunoreactivity and eventually the immunoreactivity disappears completely when the oocytes become fully mature. Interestingly, the vitellogenic oocytes of female L. ramada obtained from fresh water (captivity) contain only weak immunoreactivity of activin beta(A) compared to that of saline water (natural habitat). Moreover, immunoreactivity of activin beta(B) is observed in the phagocytic granulosa cells during resorption of oocytes (atresia) within the ovary of female reared in captivity. The immunostaining of inhibin alpha in the ovary is similar to that of activin beta(B) but the reaction intensity is weaker. However, the mature oocytes show a positive reaction for inhibin alpha. These results provide evidence for the presence of inhibin and activin-like molecules within L. ramada and reveal the distribution of these molecules in L. ramada brain-hypophysial-gonadal system. Our observations add some new informations to our current understanding of activin system in fish and suggest that inhibin and activin may play an important role for L. ramada reproduction in the event of oocyte growth and maturation.
Mousa
Mostafa A
MA
Fish Reproduction Laboratory, National Institute of Oceanography and Fisheries, Alexandria, Egypt.
Mousa
Shaaban A
SA
D000818:Animals; D001921:Brain / Q000378:metabolism*; D005260:Female; D007150:Immunohistochemistry; D028322:Inhibin-beta Subunits / Q000378:metabolism*; D007265:Inhibins / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism*; D010902:Pituitary Gland / Q000378:metabolism*; D012098:Reproduction / Q000502:physiology; D012621:Seasons; D014018:Tissue Distribution
C103008:inhibin beta A subunit; C094377:inhibin-alpha subunit; D007265:Inhibins; D028322:Inhibin-beta Subunits