[Study on rabbit mesenchymal stem cells differentiation to the adipogenic or osteogenic lineage in vitro].
Rabbit bone marrow-derived mesenchymal stem cells(MSCs) are multipotent. We studied the adipogenic and osteogenic differentiation potent using adipogenic supplement (AS) or osteogenic supplement (OS) in vitro. Specific markers of this induced adipogenic and osteogenic lineage were identified. The findings showed that the rabbit MSCs are capable of differentiating into adipogenic and osteogenic lineages spontaneously. On the 21st day, approximately 75% rabbit MSCs were induced to adipogenic or osteogenic cells in medium containing AS or OS, respectively. These results demonstrated that the differentiation of MSCs could be regulated in vitro. The underlying molecular mechanisms of adipogenic or osteogenic differentiation await elucidation.
Li
Shengfu
S
Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital of Sichuan University, Chengdu 610041.
Huang
Dingqiang
D
Lu
Xiaofeng
X
Liu
Jin
J
Sun
Minghan
M
Li
Youping
Y
Cheng
Jingqiu
J
Bu
Hong
H
Liang
Chuanyu
C
D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001842:Bone and Bones / Q000166:cytology*; D002454:Cell Differentiation; D019070:Cell Lineage; D066298:In Vitro Techniques; D008648:Mesoderm / Q000166:cytology*; D011817:Rabbits; D013234:Stem Cells / Q000166:cytology*