Induction of apoptosis and G2/M cell cycle arrest by Gleditsioside E from Gleditsia sinensis in HL-60 cells.
Gleditsioside E, a triterpene saponin isolated from Gleditsia sinensis, showed significant cytotoxicity against Bel-7402, BGC-823, HeLa, HL-60 and MCF-7 cell lines. The results of flow cytometry with annexin V-FITC/PI double staining proved that gleditsioside E mainly induced early apoptosis in HL-60 cells. Gleditsioside E treatment resulted in a prominent increase of the G 2 /M population in HL-60 cells, and an accumulation of the sub-G 1 (hypoploid) peak was observed.
Zhong
Lei
L
State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, P. R. China.
Qu
Guiqing
G
Li
Ping
P
Han
Jian
J
Guo
Dean
D
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects*; D005434:Flow Cytometry; D005638:Fruit; D031501:Gleditsia; D018922:HL-60 Cells / Q000187:drug effects; D006801:Humans; D020128:Inhibitory Concentration 50; D017239:Paclitaxel / Q000494:pharmacology; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D012503:Saponins / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D012503:Saponins; C478029:gleditsioside E; D017239:Paclitaxel