title: Direct electrochemical characterization of Vitreoscilla sp. hemoglobin entrapped in organic films.
abstract: The redox properties of a prokaryotic, Vitreoscilla sp. hemoglobin (VHb) in fuzzy organic films are studied with electrochemistry. This VHb exhibits irreversible electrochemical response at bare pyrolytic graphite (PG) electrode surfaces. However, upon being entrapped in organic films, the heterogeneous electron transfer rate of VHb will be sufficiently high to produce a quasi-reversible electrochemical response. The observation of electrocatalysis (reduction of O2) by hemes suggests that the protein can retain its biological activity under these conditions.
authors.affiliation: Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, PR China.
authors.forename: Chunhai
authors.initials: C
authors.lastname: Fan
authors.forename: Jun
authors.initials: J
authors.lastname: Zhong
authors.forename: Rong
authors.initials: R
authors.lastname: Guan
authors.forename: Genxi
authors.initials: G
authors.lastname: Li
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D002244:Carbon; D004121:Dimethyl Sulfoxide; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D006108:Graphite; D006454:Hemoglobins / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry; D011094:Polyethyleneimine; D011994:Recombinant Proteins / Q000737:chemistry; D054793:Truncated Hemoglobins
chemical_list: D001426:Bacterial Proteins; D006454:Hemoglobins; D011994:Recombinant Proteins; D054793:Truncated Hemoglobins; C009139:pyrolytic carbon; C082613:hemoglobin protein, Vitreoscilla; D002244:Carbon; D006108:Graphite; D011094:Polyethyleneimine; D010100:Oxygen; D004121:Dimethyl Sulfoxide