title: Anti-angiogenic potential of Gleditsia sinensis fruit extract.
abstract: Blood supply plays a crucial role in solid tumour development and leukaemogenesis. It has been suggested that blocking of angiogenesis could be possible in cancer therapy. We have demonstrated the antiproliferative activity of Gleditsia sinensis fruit extract (GSE) on various human solid tumour cancer cell lines as well as leukaemia cell lines and primary cultured leukaemia cells obtained from leukaemia patients. However, the antiangiogenic potential of GSE has not been demonstrated. Here we demonstrated that GSE could reduce vascular endothelial growth factor (VEGF) mRNA expression in dose- and time course-dependently in MDA-MB231 breast cancer and HepG2 hepatoblastoma cell lines as measured by reverse transcriptase polymerase chain reaction. Enzyme-linked immunosorbent assay further showed that GSE could reduce the VEGF secretion from various cancer cell lines including MDA-MB231, HepG2, HL-60 (acute promyelocytic leukaemia) and eleven primary cultured leukaemia cells obtained from acute myelogenous leukaemia patients. In vivo chick chorioallantoic membrane assay illustrated that GSE could reduce the angiogenic activity of basic fibroblast growth factor. Taken together, the information suggested that GSE could be potentially used as an angiogenic inhibitor in both solid tumour and leukaemia therapy.
authors.affiliation: Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Hong Kong, China. bclchow@polyu.edu.hk
authors.forename: Larry Ming Cheung
authors.initials: LM
authors.lastname: Chow
authors.forename: Chung Hin
authors.initials: CH
authors.lastname: Chui
authors.forename: Johnny Cheuk On
authors.initials: JC
authors.lastname: Tang
authors.forename: Fung Yi
authors.initials: FY
authors.lastname: Lau
authors.forename: Mabel Yin Chun
authors.initials: MY
authors.lastname: Yau
authors.forename: Gregory Yin Ming
authors.initials: GY
authors.lastname: Cheng
authors.forename: Raymond Siu Ming
authors.initials: RS
authors.lastname: Wong
authors.forename: Paul Bo San
authors.initials: PB
authors.lastname: Lai
authors.forename: Thomas Wai Tong
authors.initials: TW
authors.lastname: Leung
authors.forename: Ivy Tuang Ngo
authors.initials: IT
authors.lastname: Teo
authors.forename: Filly
authors.initials: F
authors.lastname: Cheung
authors.forename: Dean
authors.initials: D
authors.lastname: Guo
authors.forename: Albert Sun Chi
authors.initials: AS
authors.lastname: Chan
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000097:blood / Q000188:drug therapy*; D002642:Chick Embryo; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D016228:Endothelial Growth Factors / Q000235:genetics / Q000378:metabolism; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000187:drug effects; D005638:Fruit / Q000737:chemistry; D031501:Gleditsia / Q000737:chemistry*; D018922:HL-60 Cells / Q000187:drug effects; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D007951:Leukemia, Myeloid / Q000097:blood / Q000188:drug therapy*; D008113:Liver Neoplasms / Q000097:blood / Q000188:drug therapy*; D008222:Lymphokines / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010936:Plant Extracts / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014407:Tumor Cells, Cultured; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
chemical_list: D020533:Angiogenesis Inhibitors; D016228:Endothelial Growth Factors; D036341:Intercellular Signaling Peptides and Proteins; D008222:Lymphokines; D010936:Plant Extracts; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D016222:Fibroblast Growth Factor 2