Characterization of platelet aggregation in whole blood of laboratory animals by a screen filtration pressure method.
The characteristics of platelet aggregation of laboratory animals were investigated with whole blood and platelet-rich plasma (PRP). We measured the platelet aggregation threshold index (PATI) of whole blood and PRP aggregations induced by ADP or collagen, using a novel whole blood aggregometer, the WBA analyzer, with a screen filtration pressure (SFP) method. At 60 min after blood collection, PATI values of guinea pig, mouse, rat, dog and rabbit were 0.83, 1.78, 46.48, 49.85 and 53.42 microM for ADP-induced whole blood aggregation, respectively, whereas their PATI values for ADP-induced PRP aggregation were 1.16, 2.77, 2.65, 10.81 and 18.77 microM, respectively. These suggest that ADP-induced platelet aggregations of rat, dog and rabbit are suppressed in whole blood. PATI values of guinea pig, mouse, rat, dog and rabbit were 1.84, 0.62, 11.90, 2.34, 12.32 microg/ml for the collagen-induced whole blood aggregation, respectively, whereas their PATI values for the collagen-induced PRP aggregation were 4.21, 1.50, 5.36, 11.31, 13.30 microg/ml, respectively. Collagen-induced aggregation activity of the guinea pig, mouse and dog was significantly higher in whole blood than in the PRP. These results demonstrated that species differences in laboratory animals exist for whole blood aggregation, and that the SFP aggregometer may be useful to evaluate platelet function in various animal species.
Sudo
Toshiki
T
First Institute of New Drug Research, Otsuka Pharmaceutical Co., Ltd. 463-10, Kagasuno, Kawauchi-cho, Tokushima 771-0192, Japan. t_sudo@research.otsuka.co.jp
Ito
Hideki
H
Kimura
Yukio
Y
D000244:Adenosine Diphosphate; D000818:Animals; D001769:Blood; D003094:Collagen; D005374:Filtration; D006801:Humans; D007700:Kinetics; D009391:Nephelometry and Turbidimetry / Q000379:methods; D010949:Plasma; D010974:Platelet Aggregation; D010979:Platelet Function Tests / Q000295:instrumentation / Q000379:methods*; D013045:Species Specificity
D000244:Adenosine Diphosphate; D003094:Collagen