Antioxidative and anti-endotoxin effects of propofol on endothelial cells.
OBJECTIVE
To investigate the antioxidant and anti-endotoxin effects of propofol on endothelial cells and the possible mechanisms.


METHODS
Cultured endothelial cells were treated with hydrogen peroxide (H(2)O(2)), propofol + H(2)O(2), lipopolysaccharide (LPS) and propofol + LPS, respectively. Endothelial cell damage was monitored for possible lactic dehydrogenase (LDH) release. The transcription and the protein expression levels of endothelial nitric oxide synthase (eNOS) were measured.


RESULTS
LDH release was higher in groups treated with H(2)O(2) or LPS than in the control group. After pretreatment with propofol, the effects induced by H(2)O(2) were attenuated, but propofol did not decrease the LDH release induced by LPS. Both H(2)O(2) and LPS significantly increased the eNOS transcript levels and the increases were significantly attenuated after pretreatment with propofol. Both H(2)O(2) and LPS significantly increased the eNOS protein expression and the increase was attenuated after pretreatment with propofol.


CONCLUSIONS
Propofol could protect endothelial cells against oxidative stress by inhibiting eNOS transcription and protein expression, but could not antagonise endotoxin induced cell injuries.
Peng
Zhiyong
Z
Department of Anesthesia, Union Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430022, China. pengzy5@public.wh.hb.cn
Luo
Min
M
Ye
Shiqiao
S
Critchley
Lester A H
LA
Joynt
Gavin M
GM
Ho
Anthony M H
AM
Yao
Shanglong
S
D000975:Antioxidants / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D004731:Endotoxins / Q000037:antagonists & inhibitors*; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology; D009569:Nitric Oxide / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000096:biosynthesis; D052250:Nitric Oxide Synthase Type III; D015742:Propofol / Q000494:pharmacology*
D000975:Antioxidants; D004731:Endotoxins; D016166:Free Radical Scavengers; D008070:Lipopolysaccharides; D009569:Nitric Oxide; C496314:NOS3 protein, human; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III; D015742:Propofol