title: A novel monoclonal reagent recognizing native and denatured Vbeta5.3-related chains of human T cell receptor.
abstract: Monoclonal antibodies to specific families of TCR variable domains serve as highly useful immunochemical tools for basic research in T-cell biology and diagnosis of autoimmune diseases. Monoclonal antibody MEM-262 characterized in this communication recognizes beta chains of the TCR expressed by HPB-ALL cell line (carrying Vbeta5.3) and a small subset of peripheral blood T cells. This subset is larger than that recognized by a previously described Vbeta5.3-specific mAb. MEM-262 potently stimulates selective expansion of the T-cell subset, efficiently immunoisolates native TCR complexes as well as free beta chains and uniquely recognizes denatured TCRbeta chains under the conditions of Western blotting.
authors.affiliation: Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Vídenská 1083, 142 20 Prague 4, Czech Republic.
authors.forename: Dagmar
authors.initials: D
authors.lastname: Pavlistová
authors.forename: Karel
authors.initials: K
authors.lastname: Drbal
authors.forename: Ivan
authors.initials: I
authors.lastname: Hilgert
authors.forename: Václav
authors.initials: V
authors.lastname: Horejsí
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002460:Cell Line; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D006801:Humans; D051379:Mice; D011489:Protein Denaturation; D011948:Receptors, Antigen, T-Cell / Q000737:chemistry* / Q000276:immunology*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000737:chemistry / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D011948:Receptors, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta