[Preparation and identification of rabbit-anti-human nestin antiserum].
Nestin belongs to the class VI intermediate filament family and it is a marker for neural progenitor cells. In this work, the 3'-terminal coding sequence(396 bp) of human nestin gene was cloned into pGEX-3X plasmid and introduced into BL21 E. coli cells. The GST-nestin protein was purified with an affinity column. Anti-human nestin antiserum was raised by immunizing a rabbit with the fusion protein. The high specificity of the antibody against human nestin was confirmed by western-blot and immunocytochemistry analysis.
Liu
Lei
L
Laboratory of Genomic Physiology, Brain Research Center, LiRen Laboratory, Fudan University, Shanghai 200433.
Lin
Wan Min
WM
Lin
Jun Ji
JJ
Liu
Xuan
X
Guo
Lan Ping
LP
Xu
Ping
P
D000818:Animals; D000918:Antibody Specificity; D003001:Cloning, Molecular; D005982:Glutathione Transferase / Q000235:genetics / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000276:immunology*; D007381:Intermediate Filament Proteins / Q000235:genetics / Q000276:immunology*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000276:immunology*; D064231:Nestin; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000276:immunology* / Q000302:isolation & purification
D007106:Immune Sera; D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin; D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase