A new subfamily of the Nudix hydrolase superfamily active on 5-methyl-UTP (ribo-TTP) and UTP.
A new subfamily of the Nudix hydrolases, identified by conserved amino acids upstream and downstream of the Nudix box, has been characterized. The cloned, expressed, and purified orthologous enzymes have major activities on the non-canonical nucleoside triphosphate 5-methyl-UTP (ribo-TTP) and the canonical nucleotide UTP. In addition to their homologous signature sequences and their similar substrate specificities, the members of the subfamily are inhabitants of or are related to the bacterial rhizosphere. We propose the acronym and mnemonic, utp, for the gene designating this unique UTPase.
Xu
WenLian
W
Department of Biology, The Johns Hopkins University, Baltimore, Maryland 21218, USA.
Shen
JianYing
J
Dunn
Christopher A
CA
Bessman
Maurice J
MJ
D016960:Agrobacterium tumefaciens / Q000201:enzymology; D000595:Amino Acid Sequence; D016934:Caulobacter / Q000201:enzymology; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D011550:Pseudomonas aeruginosa / Q000201:enzymology; D011755:Pyrophosphatases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D013379:Substrate Specificity; D014544:Uridine Triphosphate / Q000378:metabolism*; D000096964:Nudix Hydrolases
D011755:Pyrophosphatases; D014544:Uridine Triphosphate; D000096964:Nudix Hydrolases; C023278:uridine triphosphatase