title: Characterization of Adoxophyes honmai single-nucleocapsid nucleopolyhedrovirus: morphology, structure, and effects on larvae.
abstract: A nucleopolyhedrovirus (NPV) was isolated from a diseased larva of the smaller tea tortrix, Adoxophyes honmai, collected from a tea field in Tsukuba, Ibaraki, Japan. Electron microscopic observations confirmed that A. honmai NPV (AdhoNPV) was a single-nucleocapsid type virus. The genome size of AdhoNPV was estimated to be 111.6 +/- 0.9kb (mean +/- SE) by restriction endonuclease analysis. AdhoNPV was also infectious to two other Adoxophyes species, the summer fruit tortrix Adoxophyes orana and Adoxophyes dubia. The LD50 values for neonatal, second, third, fourth, and fifth (final) instar larvae of A. honmai were determined as 61, 107, 688, 1,961, and 4,085 occlusion bodies/insect, respectively. Most of the infected larvae died 5-9 days after molting to the final instar, regardless of the timing of inoculation. However, when neonates were exposed to extremely high doses of AdhoNPV (greater than 100 x LD90), larval development was prevented and most of the larvae died in the first instar.
authors.affiliation: Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan. ryuki@cc.tuat.ac.jp
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Ishii
authors.forename: Madoka
authors.initials: M
authors.lastname: Nakai
authors.forename: Shohei
authors.initials: S
authors.lastname: Okuno
authors.forename: Jun
authors.initials: J
authors.lastname: Takatsuka
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Kunimi
mesh_terms: D000818:Animals; D004279:DNA, Viral / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006790:Host-Parasite Interactions; D007814:Larva / Q000821:virology; D008854:Microscopy, Electron; D009036:Moths / Q000821:virology*; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology / Q000648:ultrastructure; D010572:Pest Control, Biological; D015183:Restriction Mapping; D014764:Viral Proteins / Q000032:analysis
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins