title: [Genotyping of HLA-Cw locus in Chinese Yi ethnic group by PCR-SSP].
abstract: BACKGROUND
  To analyze the genetic polymorphism of HLA-Cw locus in Chinese Yi ethnic group by DNA typing for further study on its association with HIV infection and progression to AIDS.
  
  
  METHODS
  A rapid genotyping method for HLA-Cw by PCR-SSP was set up. It combined twenty-six specific primers and one pair of internal control primer to form twenty-four one-step reactions for each sample. Totally 102 unrelated healthy Chinese Yi ethnic individuals were typed.
  
  
  RESULTS
  Twelve HLA-Cw alleles were detected in Chinese Yi ethnic group with HLA-Cw*01, Cw*07 and Cw*08 as the most common genes, which accounted for a frequency of 0.333 3, 0.250 0 and 0.176 5 respectively; four kinds of non-serologically defined HLA-Cw genes i.e. Cw*12, Cw*1301, Cw*14 and Cw*15 were found in this population. Hardy-Weinburg test showed that the genotype distribution observed was correspondent with the expected (chi2=65.983 1, df=66, P>0.05).
  
  
  CONCLUSIONS
  This study provides the data of HLA-Cw gene frequency in Chinese Yi ethnic group, which may contribute to research on anthropology, disease association and vaccine application. The result also confirmed that PCR-SSP was a reliable and fast method for HLA-Cw genotyping.
authors.affiliation: Department of Genetic Engineering, Wuhan Institute of Biological Products, Wuhan 430060, China.
authors.forename: Xiao-zhi
authors.initials: XZ
authors.lastname: Lu
authors.forename: Kun-xue
authors.initials: KX
authors.lastname: Hong
authors.forename: Guang-ming
authors.initials: GM
authors.lastname: Qin
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Chen
authors.forename: Yu-hua
authors.initials: YH
authors.lastname: Ruan
authors.forename: Chong-hang
authors.initials: CH
authors.lastname: Li
authors.forename: Jia-hong
authors.initials: JH
authors.lastname: Zhu
authors.forename: Yi-ming
authors.initials: YM
authors.lastname: Shao
mesh_terms: D000483:Alleles; D044466:Asian People / Q000235:genetics; D002681:China / Q000208:ethnology; D005787:Gene Frequency; D005838:Genotype; D015236:HLA-C Antigens / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D011897:Random Allocation
chemical_list: D015236:HLA-C Antigens