title: Use of chitosan membrane from the carapace of the soldier crab Mictyris brevidactylus for biosensor construction.
abstract: Glucose oxidase (EC 1.3.4.3) was immobilized on chitosan membrane (<0.1 mm in thickness) prepared from the carapace of the soldier crab Mictyris brevidactylus. A glucose electrode was constructed by covering a platinum electrode (2.0 mm in diameter) with the enzyme membrane. The enzyme electrode sensed glucose amperometrically (1.0 micro A/mM, with linear range up to 0.5 mM, r = 0.999) when positively imposed with 0.6 V against an Ag/AgCl reference electrode. The glucose biosensor was sensitive (<0.1 micro M, S/N > 3), reproducible (CV for 55 micro M glucose <3%, n = 5), reagentless, and durable for months.
authors.affiliation: Department of Bio-Industrial Mechatronics Engineering, College of Agriculture, National Taiwan University, 1, Roosevelt Road, Sec. 4, Taipei, Taiwan 106, Republic of China.
authors.forename: Bo-Chuan
authors.initials: BC
authors.lastname: Hsieh
authors.forename: Tzong-Jih
authors.initials: TJ
authors.lastname: Cheng
authors.forename: Tzu-Yu
authors.initials: TY
authors.lastname: Wang
authors.forename: Richie L C
authors.initials: RL
authors.lastname: Chen
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001704:Biopolymers / Q000302:isolation & purification; D015374:Biosensing Techniques / Q000295:instrumentation*; D003386:Brachyura / Q000737:chemistry; D002686:Chitin / Q000031:analogs & derivatives* / Q000302:isolation & purification; D048271:Chitosan; D004566:Electrodes; D004800:Enzymes, Immobilized; D004867:Equipment Design; D005069:Evaluation Studies as Topic; D005947:Glucose / Q000032:analysis*; D008422:Materials Testing; D008567:Membranes, Artificial
chemical_list: D001672:Biocompatible Materials; D001704:Biopolymers; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D002686:Chitin; D048271:Chitosan; D005947:Glucose