Comparative effect of Prunus persica L. BATSCH-water extract and tacrine (9-amino-1,2,3,4-tetrahydroacridine hydrochloride) on concentration of extracellular acetylcholine in the rat hippocampus.
Prunus persica L. BATSCH seed-water extract (PPE) has been used in the treatment of the degenerative disorders, such as hypermenorrhea and dysmenorrhea, in Taiwan, China, Japan and Korea. In this study, the effects of oral administration of PPE on the extracellular acetylcholine concentration in the hippocampus of rats were evaluated, and compared to that of tacrine (9-amino-1,2,3,4-tetrahydroacridine hydrochloride), a well-known and centrally acting acetylcholinesterase (AChE) inhibitor, which had been developed for the treatment of Alzheimer's disease. We measured the inhibition of brain AChE. PPE at 2.5g/kg and tacrine at 5mg/kg showed significant effects for more than 6h. At these doses, the maximum increases were observed at about 1.5h after administration of PPE, and at about 2h with tacrine, and were 454 and 412% of the pre-level, respectively. The results suggest that oral administration of PPE and tacrine increases acetylcholine concentration in the synaptic cleft of the hippocampus mostly through AChE inhibition, and that PPE has a potent and long-lasting effect on the central cholinergic system.
Kim
Yeon-Kye
YK
National Research Laboratory for Glycobiology (NRLG), Korean Ministry of Science and Technology, Kyungju City, 780-714, Kyungbuk, South Korea. chkimbio@dongguk.ac.kr
Koo
Byung-Soo
BS
Gong
Dae-Jong
DJ
Lee
Young-Choon
YC
Ko
Jeong-Heon
JH
Kim
Cheorl-Ho
CH
D000109:Acetylcholine / Q000378:metabolism*; D000110:Acetylcholinesterase / Q000378:metabolism; D000284:Administration, Oral; D000818:Animals; D002800:Cholinesterase Inhibitors / Q000378:metabolism / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D017551:Microdialysis; D010936:Plant Extracts / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D027861:Prunus / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D013619:Tacrine / Q000378:metabolism / Q000494:pharmacology*; D013997:Time Factors; D014867:Water
D002800:Cholinesterase Inhibitors; D010936:Plant Extracts; D014867:Water; D013619:Tacrine; D000110:Acetylcholinesterase; D000109:Acetylcholine