title: Clinically established hemostatic scaffold (tissue fleece) as biomatrix in tissue- and organ-engineering research.
abstract: Various types of three-dimensional matrices have been used as basic scaffolds in myocardial tissue engineering. Many of those are limited by insufficient mechanical function, availability, or biocompatibility. We present a clinically established collagen scaffold for the development of bioartificial myocardial tissue. Neonatal rat cardiomyocytes were seeded into Tissue Fleece (Baxter Deutschland, Heidelberg, Germany). Histological and ultrastructural examinations were performed by DAPI and DiOC(18) staining and electron microscopy, respectively. Force measurements from the spontaneously beating construct were obtained. The constructs were stimulated with agents such as adrenalin and calcium, and by stretching. Passive stretch curves were obtained. Spontaneous contractions of solid bioartificial myocardial tissue (BMT), 20 x 15 x 2 mm, resulted. Contractions continued to week 12 (40% of BMTs) in culture. Histology revealed intercellular and also cell-fibril junctions. Elasticity was similar to that of native rat myocardium. Contractile force increased after topical administration of Ca(2+) and adrenaline. Stretch led to the highest levels of contractile force. In summary, bioartificial myocardial tissue with significant in vitro longevity, spontaneous contractility, and homogeneous cell distribution was produced using Tissue Fleece. Tissue Fleece constitutes an effective scaffold to engineer solid organ structures, which could be used for repair of congenital defects or replacement of diseased tissue.
authors.affiliation: Division of Thoracic and Cardiovascular Surgery and Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Hannover Medical School, Hannover, Germany. tkofidis@stanford.edu
authors.forename: T
authors.initials: T
authors.lastname: Kofidis
authors.forename: P
authors.initials: P
authors.lastname: Akhyari
authors.forename: B
authors.initials: B
authors.lastname: Wachsmann
authors.forename: K
authors.initials: K
authors.lastname: Mueller-Stahl
authors.forename: J
authors.initials: J
authors.lastname: Boublik
authors.forename: A
authors.initials: A
authors.lastname: Ruhparwar
authors.forename: H
authors.initials: H
authors.lastname: Mertsching
authors.forename: L
authors.initials: L
authors.lastname: Balsam
authors.forename: R
authors.initials: R
authors.lastname: Robbins
authors.forename: A
authors.initials: A
authors.lastname: Haverich
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D003094:Collagen; D009206:Myocardium; D051381:Rats; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; D003094:Collagen