Effect of recombinant human basic fibroblast growth factor on angiogenesis during mandible fracture healing in rabbits.
OBJECTIVE
To investigate the effect of recombinant human basic fibroblast growth factor (rhbFGF) on angiogenesis during mandible fracture healing in rabbit.


METHODS
Fifty adult white rabbits were used for animal model and randomly divided into a control group (25 rabbits) and an experimental group (25 rabbits). The membranous complex of rhbFGF and bovine type I collagen was prepared and implanted into the rabbit mandible fracture site under periosteum. The animals were sacrificed on 7, 14, 28, 56 and 84 days respectively after operation and the whole mandibles were harvested. The expression of factor VIII related antigen (F8-RA) in callus was examined with immunohistochemical staining.


RESULTS
The amounts of microvascular formation in calluses in the rhbFGF-treating group on days 7, 14, 28 and 56 were more than those of the control group (P<0.01).


CONCLUSIONS
The results indicated that rhbFGF could stimulate microvascular formation during mandible fracture healing in rabbits.
Gong
Zhen-yu
ZY
Department of Oral-Maxillofacial Surgery, College of Stomatology, Fourth Military Medical University, Xi'an 710032, China.
Zhou
Shu-xia
SX
Gu
Xiao-ming
XM
Li
Di-chen
DC
Sun
Ming-lin
ML
D000818:Animals; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D017102:Fracture Healing / Q000502:physiology*; D008337:Mandibular Fractures / Q000503:physiopathology*; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D011817:Rabbits; D011994:Recombinant Proteins / Q000494:pharmacology
D011994:Recombinant Proteins; D016222:Fibroblast Growth Factor 2