Differences in delta9 desaturase activity between Jersey- and Limousin-sired cattle.
An experiment examined delta9 desaturase activity and FA composition in subcutaneous adipose tissue in two differing breeds of cattle. Jersey-sired cattle had significantly higher rates of desaturase activity than Limousin-sired cattle (1.55 vs. 0.75 nmol/mg protein/min). This difference was also demonstrated by a lower concentration of individual (e.g., 18:0) and total saturated FA (38.3 vs. 45.1 wt%), and a higher concentration of individual (e.g., 16:1) and total monounsaturated FA (58.2 vs. 52.7 wt%) in the Jersey animals. Other indices of desaturation calculated from the FA composition showed this same difference. The slip point of adipose tissue of Jersey cattle (36.8 degrees C) was significantly lower than that of Limousin cattle (39.2 degrees C), but Jersey adipose tissue had a greater content of beta-carotene. The positive relationship between adipose tissue beta-carotene and desaturation opposes the negative relationship between dietary beta-carotene and desaturation determined elsewhere. These results, however, lead to the hypothesis that some cattle have a reduced capacity to metabolize beta-carotene to various forms of vitamin A, a compound that can reduce delta9 desaturase enzyme activity. In addition, the higher level of intramuscular fat in Jersey cattle (6.97 vs. 3.82%) is possibly related to a lack of inhibition of the adipocyte differentiation genes by vitamin A.
Siebert
B D
BD
Department of Animal Science, University of Adelaide, Roseworthy Campus, SA 5371, Australia. brian.siebert@adelaide.edu.au
Pitchford
W S
WS
Kruk
Z A
ZA
Kuchel
H
H
Deland
M P B
MP
Bottema
C D K
CD
D000273:Adipose Tissue / Q000737:chemistry / Q000201:enzymology; D000818:Animals; D002417:Cattle / Q000235:genetics / Q000378:metabolism*; D003433:Crosses, Genetic; D005227:Fatty Acids / Q000032:analysis*; D005260:Female; D008055:Lipids / Q000737:chemistry; D008297:Male; D009132:Muscles / Q000737:chemistry; D013045:Species Specificity; D013230:Stearoyl-CoA Desaturase / Q000378:metabolism*; D040521:Subcutaneous Tissue / Q000737:chemistry* / Q000201:enzymology; D019207:beta Carotene / Q000032:analysis
D005227:Fatty Acids; D008055:Lipids; D019207:beta Carotene; D013230:Stearoyl-CoA Desaturase