title: Deficiency of red cell bound CD55 and CD59 in patients with systemic lupus erythematosus.
abstract: CD55 and CD59 are glycosylphosphatidylinositol-anchored proteins with complement inhibitory properties. Autoimmune hemolytic anemia (AIHA) has been associated with antiphospholipid antibodies (APLA). The aim of this study was to evaluate the presence of APLA and its possible correlation with diminished CD55 and CD59 in red blood cells from patients with primary AIHA or secondary to systemic lupus erythematosus (SLE). Flow cytometric analyses were performed on CD55 and CD59 stained erythrocytes from 24 patients (primary AIHA, n=8; AIHA plus SLE, n=11; and SLE without AIHA, n=5) and 20 healthy controls. Antibodies to several phospholipids were detected in the sera by ELISA. Most patients with AIHA plus SLE and few with primary AIHA showed deficiency of either or both CD55 and CD59 expression and was not associated to the presence of APLA, while SLE patients exhibited a normal expression of these molecules. Although our findings showed CD55 and CD59 deficiency in primary or secondary AIHA, it appears that this defect plays a facilitator rather than a triggering role for the hemolytic process. Additionally, a role of anti-phospholipid antibodies as causative of this acquired defect is questionable.
authors.affiliation: Department of Immunology and Rheumatology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga No. 15, Tlalpan, 14000 Mexico City DF, Mexico. lllyrp@quetzal.innsz.mx
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Richaud-Patin
authors.forename: Beatriz
authors.initials: B
authors.lastname: Pérez-Romano
authors.forename: Eduardo
authors.initials: E
authors.lastname: Carrillo-Maravilla
authors.forename: Alma B
authors.initials: AB
authors.lastname: Rodriguez
authors.forename: Abraham J
authors.initials: AJ
authors.lastname: Simon
authors.forename: Javier
authors.initials: J
authors.lastname: Cabiedes
authors.forename: Juan
authors.initials: J
authors.lastname: Jakez-Ocampo
authors.forename: Luis
authors.initials: L
authors.lastname: Llorente
authors.forename: Alejandro
authors.initials: A
authors.lastname: Ruiz-Argüelles
mesh_terms: D000328:Adult; D000744:Anemia, Hemolytic, Autoimmune / Q000097:blood / Q000276:immunology; D018958:CD55 Antigens / Q000097:blood* / Q000276:immunology*; D018957:CD59 Antigens / Q000097:blood* / Q000276:immunology*; D016022:Case-Control Studies; D004912:Erythrocytes / Q000276:immunology / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D006461:Hemolysis; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood* / Q000276:immunology*; D008297:Male; D008875:Middle Aged
chemical_list: D018958:CD55 Antigens; D018957:CD59 Antigens