Recovery of poly(3-hydroxybutyrate) from high cell density culture of Ralstonia eutropha by direct addition of sodium dodecyl sulfate.
A simple and effective method for the recovery poly(3-hydroxybutyrate) [P(3HB)] directly from high cell density culture broth with no pretreatment steps has been developed. This method consists of direct addition of sodium dodecyl sulfate (SDS) to the culture broth, shaking, heat treatment, and washing steps. When the SDS/biomass ratio was higher than 0.4, the purity of recovered P(3HB) was over 95% for various cell concentrations of 50-300 g dry cell l(-1), with the highest value of 97%. The recovery of P(3HB) was over 90% regardless of cell concentration and SDS dosage (SDS/biomass ratios, 0.1-0.7). One g SDS digests 0.72 g non-P(3HB) cell materials. The reduction in molecular weight, due to degradation of P(3HB) by SDS, was negligible.
Kim
Mia
M
Department of Environmental Science and Engineering, Ewha Womans University, 11-1 Daehyon-dong, Seodaemun-gu, Seoul 120-750, Korea.
Cho
Kyung-Suk
KS
Ryu
Hee Wook
HW
Lee
Eun Gyo
EG
Chang
Yong Keun
YK
D019149:Bioreactors / Q000145:classification / Q000382:microbiology; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media / Q000302:isolation & purification / Q000494:pharmacology; D020118:Cupriavidus necator / Q000187:drug effects* / Q000302:isolation & purification / Q000378:metabolism*; D005240:Feasibility Studies; D006885:Hydroxybutyrates / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D008970:Molecular Weight; D011091:Polyesters / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D011786:Quality Control; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D000094122:Polyhydroxybutyrates
D003470:Culture Media; D006885:Hydroxybutyrates; D011091:Polyesters; D012967:Sodium Dodecyl Sulfate; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates