title: Copper complex of hydroxyl-substituted triazamacrocyclic ligand and its antitumor activity.
abstract: The protonation constants and the stability constants for the formation of copper (II) complex of the ligand [1,4,7] Triazecan-9-ol (L) were presented. Antitumor activity of CuL complex was reported. Preliminary pharmacological tests showed that it had antitumor activity against HXO-RB44 and BEL-7402 cell lines in vitro. Nuclei of [CuL]-stimulated BEL-7402 cells clearly exhibited condensation and break down into chromatin clumps typical of apoptosis. Also it exhibited perturbation effects to BEL-7402 cell lines cycle and further studies showed that it could cleave supercoiled DNA (pBR 322) to nicked and linear DNA.
authors.affiliation: Department of Chemistry, Wuhan University, Wuhan 430072, PR China. chemoliangf@yahoo.com.cn
authors.forename: Feng
authors.initials: F
authors.lastname: Liang
authors.forename: Chengtai
authors.initials: C
authors.lastname: Wu
authors.forename: Huakuan
authors.initials: H
authors.lastname: Lin
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.forename: Dongzhao
authors.initials: D
authors.lastname: Gao
authors.forename: Zhaoyang
authors.initials: Z
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Wei
authors.forename: Congyi
authors.initials: C
authors.lastname: Zheng
authors.forename: Mengxiang
authors.initials: M
authors.lastname: Sun
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D006690:Bisbenzimidazole; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D004396:Coloring Agents; D003300:Copper / Q000737:chemistry; D004273:DNA, Neoplasm / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D004591:Electrophoresis, Polyacrylamide Gel; D007202:Indicators and Reagents; D008024:Ligands; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000494:pharmacology*; D011199:Potentiometry
chemical_list: D000970:Antineoplastic Agents; D004396:Coloring Agents; D004273:DNA, Neoplasm; D007202:Indicators and Reagents; D008024:Ligands; D009942:Organometallic Compounds; D003300:Copper; D006690:Bisbenzimidazole