[The effect of nitric oxide and total antioxide capacity on the germ cell apoptosis in the rat cryptorchid].
OBJECTIVE
To study the effect of nitric oxide (NO) and total antioxide capacity (T-AOC) on the germ cell apoptosis in the cryptorchidism rat.


METHODS
Immature rats (22 day-olds Sprague Dawley) were selected to make the model of unilateral cryptorchid. Germ cell apoptosis was examined by terminal-deoxynucleotidyl transferase mediated d-UTP nick end labeling (TUNEL). Biochemical parameters (NO, T-AOC) were evaluated with spectrophotometric determination.


RESULTS
At the seventh day after the operation, as compared to the normal side testis, the number of apoptotic germ cells increased significantly in the undescended testis (P < 0.01), but the testes weight decreased predominantly; the NO level in the undescended testis was significantly higher than that in the normal side testis, and the T-AOC level was predominantly lower (P < 0.01).


CONCLUSIONS
Experimentally-induced cryptorchidism can lead to the increase of grem cell apoptosis in rat. The results is closely related to the increase of No level and the decrease of T-AOC.
Li
Shi-Wen
SW
Department of Urology & Andrology, Second Affiliated Hospital, Wuhan University, Wuhan, Hubei 430071, China.
Zheng
Xin-Min
XM
Zheng
Hang
H
Hu
Li-Quan
LQ
Zhu
Xin-Shen
XS
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D017209:Apoptosis; D003456:Cryptorchidism / Q000378:metabolism / Q000473:pathology*; D015227:Lipid Peroxidation; D008297:Male; D009569:Nitric Oxide / Q000502:physiology*; D009929:Organ Size; D051381:Rats; D017207:Rats, Sprague-Dawley; D013094:Spermatozoa / Q000166:cytology*
D000975:Antioxidants; D009569:Nitric Oxide