Enhanced production of antimicrobial sesquiterpenes and lipoxygenase metabolites in elicitor-treated hairy root cultures of Solanum tuberosum.
Potato (Solanum tuberosum) hairy root cultures, established by infecting potato tuber discs with Agrobacterium rhizogenes, were used as a model system for the production of antimicrobial sesquiterpenes and lipoxygenase (LOX) metabolites. Of the four sesquiterpene phytoalexins (rishitin, lubimin, phytuberin and phytuberol) detected in elicitor-treated hairy root cultures, rishitin (213 micrograms g-1 dry wt) was the most predominant followed by lubimin (171 micrograms g-1 dry wt). The elicitors also induced LOX activity (25-fold increase) and LOX metabolites, mainly 9-hydroxyoctadecadienoic acid and 9-hydroxyoctadecatrienoic acid, in potato hairy root cultures. The combination of fungal elicitor plus cyclodextrin was the most effective elicitor treatment, followed by methyl jasmonate plus cyclodextrin in inducing sesquiterpenes and LOX metabolites.
Komaraiah
P
P
School of Life Sciences, University of Hyderabad, Hyderabad 500 046, India.
Reddy
G V
GV
Reddy
P Srinivas
PS
Raghavendra
A S
AS
Ramakrishna
S V
SV
Reddanna
P
P
D000085:Acetates / Q000494:pharmacology; D000890:Anti-Infective Agents; D002457:Cell Extracts / Q000494:pharmacology; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D003505:Cyclodextrins / Q000494:pharmacology; D003517:Cyclopentanes / Q000494:pharmacology; D008041:Linoleic Acids; D044243:Linoleic Acids, Conjugated; D008042:Linolenic Acids; D008084:Lipoxygenase / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D054883:Oxylipins; D010936:Plant Extracts; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology; D011786:Quality Control; D012232:Rhizoctonia / Q000166:cytology / Q000378:metabolism; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D011198:Solanum tuberosum / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology; D013729:Terpenes; D047392:beta-Cyclodextrins; D000094162:Phytoalexins
D000085:Acetates; D000890:Anti-Infective Agents; D002457:Cell Extracts; D003470:Culture Media; D003505:Cyclodextrins; D003517:Cyclopentanes; D008041:Linoleic Acids; D044243:Linoleic Acids, Conjugated; D008042:Linolenic Acids; D054883:Oxylipins; D010936:Plant Extracts; D012717:Sesquiterpenes; D013729:Terpenes; D047392:beta-Cyclodextrins; C024347:9-hydroxy-10,12-octadecadienoic acid; C062142:9-hydroxy-10,12,15-octadecatrienoic acid; C072239:methyl jasmonate; D008084:Lipoxygenase; C031215:betadex; D000094162:Phytoalexins