Different roles for the stress-activated protein kinase pathway in the regulation of trehalose metabolism in Schizosaccharomyces pombe.
The Wis1p-Sty1p mitogen-activated protein kinase cascade is a major signalling system in the fission yeast Schizosaccharomyces pombe for a wide range of stress responses. It is known that trehalose functions as a protective metabolite to counteract deleterious effects of environmental stresses. Herein it is reported that the expression of genes related to trehalose metabolism in S. pombe, ntp1(+) (neutral trehalase) and tps1(+) [trehalose-6-phosphate (T6P) synthase], is partially regulated by the Sty1p kinase under salt-induced osmotic stress and conditions of slight oxidative stress and is fully dependent on this kinase under severe oxidative stress. This control is carried out through transcription factors Atf1p/Pcr1p during osmotic stress and through Pap1p during exposure to low levels of oxidative stress. However, all three transcription factors are needed for gene expression under conditions of extreme oxidative stress. In addition, a role for Sty1p in the modulation of post-transcriptional activation of trehalase mediated by Pka1p/Sck1p kinases, as well as in the activity of T6P synthase under such stressful conditions has been demonstrated. These results reveal a novel dual action of the Wis1p-Sty1p pathway in the regulation of trehalose metabolism in fission yeast.
Paredes
V
V
Department of Genetics and Microbiology, Facultad de Biología, University of Murcia, 30071 Murcia, Spain.
Franco
A
A
Department of Genetics and Microbiology, Facultad de Biología, University of Murcia, 30071 Murcia, Spain.
Soto
T
T
Department of Genetics and Microbiology, Facultad de Biología, University of Murcia, 30071 Murcia, Spain.
Vicente-Soler
J
J
Department of Genetics and Microbiology, Facultad de Biología, University of Murcia, 30071 Murcia, Spain.
Gacto
M
M
Department of Genetics and Microbiology, Facultad de Biología, University of Murcia, 30071 Murcia, Spain.
Cansado
J
J
Department of Genetics and Microbiology, Facultad de Biología, University of Murcia, 30071 Murcia, Spain.
D051696:Activating Transcription Factors; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004789:Enzyme Activation; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000235:genetics; D020929:Mitogen-Activated Protein Kinase Kinases / Q000235:genetics / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics / Q000378:metabolism; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014198:Trehalase / Q000235:genetics / Q000378:metabolism; D014199:Trehalose / Q000378:metabolism*
D051696:Activating Transcription Factors; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; C097701:Pcr1 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors; D014199:Trehalose; D005964:Glucosyltransferases; C023180:trehalose-6-phosphate synthase; D011494:Protein Kinases; C107804:Sck1 protein, S pombe; D020928:Mitogen-Activated Protein Kinases; C095409:sty1 protein, S pombe; D020929:Mitogen-Activated Protein Kinase Kinases; C072068:wis1 protein, S pombe; C464880:Ntp1p protein, S pombe; D010744:Phosphoric Monoester Hydrolases; D014198:Trehalase