Crystallization and preliminary X-ray crystallographic studies of mavicyanin from Cucurbita pepo medullosa.
Mavicyanin isolated from Cucurbita pepo medullosa is a glycosylated protein containing a single polypeptide chain of 109 amino-acid residues and is a member of the phytocyanin subclass of cupredoxins. Non-glycosylated recombinant mavicyanin, which was expressed in Escherichia coli, was crystallized by the hanging-drop vapour-diffusion method with ammonium sulfate as the precipitant at pH 5.5. The crystals belonged to the hexagonal space group P6(1) (or P6(5)), with unit-cell parameters a = 64.0, c = 245.0 A, four molecules per asymmetric unit and a solvent content of 59%. X-ray diffraction data were collected to 1.6 A resolution. To solve the structure of mavicyanin, the MAD method as well as a Patterson search method using the structure of stellacyanin as a starting model are presently being utilized.
Xie
Yong
Y
Department of Materials Chemistry, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan.
Inoue
Tsuyoshi
T
Miyamoto
Youichi
Y
Matsumura
Hiroyoshi
H
Kataoka
Kunishige
K
Yamaguchi
Kazuya
K
Suzuki
Shinnichiro
S
Kai
Yasushi
Y
D000645:Ammonium Sulfate / Q000737:chemistry; D018360:Crystallography, X-Ray / Q000379:methods; D004058:Diffusion; D004926:Escherichia coli / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008667:Metalloproteins / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D014675:Vegetables / Q000378:metabolism*
D008667:Metalloproteins; D010940:Plant Proteins; C022828:mavicyanin protein, Cucurbita pepo; C003343:stellacyanin protein, plant; D000645:Ammonium Sulfate