title: [Detection of bcr/abl fusion gene in chronic myeloid leukemia by fluorescence in situ hybridization and nested reverse transcription polymerase chain reaction].
abstract: OBJECTIVE
  To detect the bcr/abl fusion gene in chronic myeloid leukemia (CML) for assisting in clinical diagnosis.
  
  
  METHODS
  Fluorescence in situ hybridization (FISH) and nested reverse transcription polymerase chain reaction (RT-PCR) were used.
  
  
  RESULTS
  Out of 16 CML patients, the results of FISH was not consistent with that of nested RT-PCR in 3 cases. When compared with the results of Northern blot, it was found that nested RT-PCR was more sensitive than FISH, but might give false positive results. Moreover, FISH was the easier method for detecting rarer types of bcr/abl transcripts. For detecting the latter occasions nested RT-PCR have to design several specific primers.
  
  
  CONCLUSIONS
  It could take their advantages of applying FISH and nested RT-PCR at the same time to make the results more accurate and reliable for the diagnosis, prognosis and monitor of minimal residual disease.
authors.affiliation: Department of Hematology, Nanfang Hospital, the First Military Medical University, Guangzhou 510515, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
authors.forename: Shu-yun
authors.initials: SY
authors.lastname: Zhou
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Liu
authors.forename: Lan-lin
authors.initials: LL
authors.lastname: Song
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D005260:Female; D016044:Fusion Proteins, bcr-abl / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D008297:Male; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D016044:Fusion Proteins, bcr-abl