title: Tropomodulin binds to filensin intermediate filaments.
abstract: Tropomodulin (Tmod) is an actin filament pointed end capping protein found in the membrane skeleton of lens fiber cells. We demonstrate that Tmod4 is able to bind the lens-specific intermediate filament protein, filensin, in either co-sedimentation or solid phase binding assays in a saturable fashion, but with low affinity and stoichiometry. Furthermore, Tmod4 does not bind the 53 kDa rod domain of filensin, nor to CP49, the obligate assembly partner of filensin. Finally, the binding of filensin to Tmod4 does not inhibit the actin capping activity of Tmod4 in vitro, suggesting that the two functions are not mutually exclusive.
authors.affiliation: Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA. bfischer@scripps.edu <bfischer@scripps.edu>
authors.forename: R S
authors.initials: RS
authors.lastname: Fischer
authors.forename: R A
authors.initials: RA
authors.lastname: Quinlan
authors.forename: V M
authors.initials: VM
authors.lastname: Fowler
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D002352:Carrier Proteins / Q000378:metabolism*; D002417:Cattle; D005136:Eye Proteins / Q000378:metabolism*; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D007382:Intermediate Filaments / Q000378:metabolism*; D007700:Kinetics; D007908:Lens, Crystalline / Q000378:metabolism; D008840:Microfilament Proteins; D008970:Molecular Weight; D011485:Protein Binding; D051348:Tropomodulin
chemical_list: D002352:Carrier Proteins; D005136:Eye Proteins; D007381:Intermediate Filament Proteins; D008840:Microfilament Proteins; D051348:Tropomodulin; C079098:filensin