title: Low lung volume alters contractile properties of airway smooth muscle in sheep.
abstract: Breathing at volumes lower than functional residual capacity (FRC) can induce changes in nonasthmatic airways consistent with the behaviour of asthmatic airways. This study investigated the chronic effect of breathing at volumes lower than FRC on the contractility of airway smooth muscle and myosin light chain kinase (MLCK) content and activity. Sheep of three age groups (neonate, adolescent and adult) had their FRC reduced by approximately 25%, for 4 weeks using a leather corset. Contractile responses to carbachol were then recorded in isolated tracheal strips and bronchial rings. MLCK content and activity were assessed by immunoblotting. The rate of stress generation increased in the bronchial smooth muscle of both adult and adolescent but not neonatal corseted sheep: adolescent corseted versus control, 65.0 +/- 4.1 versus 103.4 +/- 7.0 s (to reach 50% maximum stress), respectively; and adult corseted versus control, 57.0 +/- 6.4 versus 93.4 +/- 8.2 s, respectively. This was not due to increases in either bronchial or tracheal smooth muscle amount or MLCK content and activity. The present results indicate that chronic breathing at low lung volumes increases the rate of stress generation in airway smooth muscle.
authors.affiliation: Woolcock Institute of Medical Research, Royal North Shore Hospital Campus, Sydney, New South Wales, Australia.
authors.forename: M A
authors.initials: MA
authors.lastname: McClean
authors.forename: M J
authors.initials: MJ
authors.lastname: Matheson
authors.forename: K
authors.initials: K
authors.lastname: McKay
authors.forename: P R A
authors.initials: PR
authors.lastname: Johnson
authors.forename: A-C
authors.initials: AC
authors.lastname: Rynell
authors.forename: A J
authors.initials: AJ
authors.lastname: Ammit
authors.forename: J L
authors.initials: JL
authors.lastname: Black
authors.forename: N
authors.initials: N
authors.lastname: Berend
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D008176:Lung Volume Measurements; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology*; D009130:Muscle, Smooth / Q000187:drug effects / Q000503:physiopathology*; D009219:Myosin-Light-Chain Kinase / Q000378:metabolism*; D010766:Phosphorylation; D012756:Sheep; D013312:Stress, Physiological / Q000503:physiopathology*; D013990:Tidal Volume
chemical_list: D009219:Myosin-Light-Chain Kinase