title: Suspension culture of hematopoietic stem cells in stirred bioreactors.
abstract: Hematopoietic stem cells have applications in bone marrow transplantations for the treatment of hematopoietic disorders. When murine hematopoietic stem cells were cultured in 50 ml stirred bioreactors for 14 d, stem-cell-antigen-1 positive cells (hematopoietic primitive progenitor cells) and long-term culture-initiating cells (hematopoietic stem cells) grew by 5-fold and 4-fold, respectively. These results show the possibility of growing hematopoietic stem cells using a stirred bioreactor.
authors.affiliation: Department of Chemical Engineering, Hanyang University, Seoul, Korea.
authors.forename: Joon
authors.initials: J
authors.lastname: Kwon
authors.forename: Byung-Soo
authors.initials: BS
authors.lastname: Kim
authors.forename: Mi-Jung
authors.initials: MJ
authors.lastname: Kim
authors.forename: Hong-Woo
authors.initials: HW
authors.lastname: Park
mesh_terms: D000818:Animals; D019149:Bioreactors; D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D005240:Feasibility Studies; D005434:Flow Cytometry / Q000379:methods; D006412:Hematopoietic Stem Cells / Q000145:classification / Q000166:cytology* / Q000502:physiology*; D051379:Mice