Selective enhancement of multipotential hematopoietic progenitors in vitro and in vivo by IL-20.
In a search for novel growth factors, we discovered that human interleukin-20 (IL-20) enhanced colony formation by CD34+ multipotential progenitors. IL-20 had no effect on erythroid, granulocyte-macrophage, or megakaryocyte progenitors. IL-20 transgenic mice increased the numbers and cell cycling of multipotential but not other progenitors. IL-20 administration to normal mice significantly increased only multipotential progenitor cells, demonstrating that IL-20 significantly influences hematopoiesis, with specificity toward multipotential progenitors. This is the first cytokine with such specificity identified.
Liu
Ling
L
Lilly Research Laboratories, Lilly Corporate Center, Indianapolis, IN 46285, USA. liu_ling@lilly.com
Ding
Chunjin
C
Zeng
Wei
W
Heuer
Josef G
JG
Tetreault
Jonathan W
JW
Noblitt
Timothy W
TW
Hangoc
Giao
G
Cooper
Scott
S
Brune
Kellie A
KA
Sharma
Ganesh
G
Fox
Niles
N
Rowlinson
Scott W
SW
Rogers
Danise P
DP
Witcher
Derrick R
DR
Lambooy
Peter K
PK
Wroblewski
Victor J
VJ
Miller
James R
JR
Broxmeyer
Hal E
HE
D000818:Animals; D018952:Antigens, CD34; D001854:Bone Marrow Cells / Q000166:cytology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology; D006410:Hematopoiesis / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D007378:Interleukins / Q000008:administration & dosage / Q000494:pharmacology*; D051379:Mice; D008822:Mice, Transgenic; D039902:Multipotent Stem Cells / Q000166:cytology / Q000187:drug effects*
D018952:Antigens, CD34; D007378:Interleukins; C422042:interleukin 20