title: [Experimental study on cardiac protection and anti-oxidation effects of jisheng injection].
abstract: OBJECTIVE
  To observe the effect of Jisheng injection (JSI) in protecting heart.
  
  
  METHODS
  Isolated heart of rat was preserved in modified Euro-Collins solution (mEC) containing JSI for 20 hrs, and that preserved in simple mEC was taken as control. Then Langendorff isolated rat heart perfusion was conducted. Forty minutes after perfusion, the cardiac function, coronary flow, myocardial water content were determined, and lactate dehydrogenase (LDH), creatine kinase (CK) activity in perfusate, superoxide dismutase (SOD) activity, malondialdehyde (MDA) content in myocardial tissue and pathologic change in myocardium were also observed.
  
  
  RESULTS
  The cardiac function and coronary flow of isolated heart preserved in JSI containing mEC was significantly better than those in the control (P < 0.01), with the LDH, CK activity and MDA content significantly lower (P < 0.01 and P < 0.05), SOD activity significantly higher (P < 0.05) and pathologic injury milder than those in control, but comparison of cardiac water content between the two groups showed insignificant difference.
  
  
  CONCLUSIONS
  JSI has good cardiac protective and anti-oxidizing effects.
authors.affiliation: Department of Physiology, Beijing University of TCM, Beijing 100029. zhangsqing@sina.com.cn
authors.forename: Su-qing
authors.initials: SQ
authors.lastname: Zhang
authors.forename: Xue-jun
authors.initials: XJ
authors.lastname: Shi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006321:Heart; D008297:Male; D009926:Organ Preservation; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D020940:Reishi / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D019188:Organ Preservation Solutions; D013482:Superoxide Dismutase