title: Pex10p links the ubiquitin conjugating enzyme Pex4p to the protein import machinery of the peroxisome.
abstract: The protein import machinery of the peroxisome consists of many proteins, collectively called the peroxins. By applying the split-ubiquitin technique we systematically tested the pair-wise interactions between the Nub- and Cub-labeled peroxins for the first time in the living cells of the yeast Saccharomyces cerevisiae. We found that Pex10p plays a central role in the protein interaction network by connecting the ubiquitin conjugation enzyme Pex4p to the other members of the protein import machinery. A yeast strain harboring a deletion of PEX3 enabled us to estimate the influence of the peroxisomal membrane on the formation of a subset of the investigated protein-protein interactions.
authors.affiliation: Ruhr-Universität Bochum, Institut für Physiologische Chemie, Medizinische Fakultät, 44780 Bochum, Germany.
authors.forename: Jörg H
authors.initials: JH
authors.lastname: Eckert
authors.forename: Nils
authors.initials: N
authors.lastname: Johnsson
mesh_terms: D001692:Biological Transport; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D008958:Models, Molecular; D000074401:Peroxins; D000074428:Peroxisomal Biogenesis Factor 2; D020675:Peroxisomes / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases
chemical_list: D005656:Fungal Proteins; D008565:Membrane Proteins; C492057:PEX10 protein, S cerevisiae; C491335:PEX12 protein, S cerevisiae; C070895:PEX3 protein, S cerevisiae; C076587:PEX4 protein, S cerevisiae; D000074401:Peroxins; D018160:Receptors, Cytoplasmic and Nuclear; D029701:Saccharomyces cerevisiae Proteins; D000074428:Peroxisomal Biogenesis Factor 2; D044767:Ubiquitin-Protein Ligases