Molecular probe for selective detection of thiols in water of neutral pH.
The ternary complex, [Zn(cyclen)(lumazine)](+) that can be readily obtained by mixing cyclen, zinc perchlorate, and lumazine in water in a 1:1:1 molar ratio serves as a molecular probe that detects selectively thiols in an aqueous solution of neutral pH. Thiols successfully displace the lumazine in the molecular probe, which is accompanied by a decrease of the fluorescence. The molecular probe is useful for identification of cysteine among essential amino acids and detection of glutathione in aqueous solution of neutral pH.
Kim
Dong H
DH
Center for Integrated Molecular Systems and Department of Chemistry, Division of Molecular and Life Sciences, Pohang University of Science and Technology, San 31 Hyoja-dong, Pohang 790-784, South Korea. dhkim@postech.ac.kr
Han
Min Su
MS
D000596:Amino Acids / Q000737:chemistry; D003545:Cysteine / Q000737:chemistry; D005978:Glutathione / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007700:Kinetics; D009942:Organometallic Compounds / Q000737:chemistry*; D011621:Pteridines / Q000737:chemistry*; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013438:Sulfhydryl Compounds / Q000032:analysis*; D014867:Water / Q000032:analysis*
D000596:Amino Acids; D007202:Indicators and Reagents; D009942:Organometallic Compounds; D011621:Pteridines; D012996:Solutions; D013438:Sulfhydryl Compounds; C482467:Zn(cyclen)(lumazine); D014867:Water; D005978:Glutathione; D003545:Cysteine