title: Cellular origin of IFN-gamma essential for hair cycle in normal skin.
abstract: Hair growth abnormalities in mice usually are accompanied by histologic abnormalities as well. Recently, however, we reported a mouse model in which an arrest of the hair cycle and diffuse shedding of the hair without pathologic features induced alopecia in interferon-gamma(-/-) (IFN-gamma(-/-)) C57BL/6 (B6) mice. Here, we explored the cellular origin of IFN-gamma. When bone marrow from IFN-gamma(-/-) B6 mice was transplanted into lethally irradiated IFN-gamma(+/+) B6 mice, the level of IFN-gamma mRNA expression in the skin or peripheral blood mononuclear cells (PBMCs) of recipient mouse was markedly reduced, suggesting that IFN-gamma is normally produced by bone marrow-derived cells. Although severe combined immunodeficiency (SCID) mice lack mature T cells and B cells, IFN-gamma-dependent hair regrowth was induced in SCID mice by depilation, which caused alopecia in IFN-gamma(-/-) B6 mice. Consistently, IFN-gamma mRNA expression in the skin or PBMC from SCID mice was comparable to that from their genetic counterpart (BALB/c mice), suggesting IFN-gamma production by non-T cells. RT-PCR analyses after separation of PBMC from SCID mice into eight fractions by a cell sorter revealed that Mac-1(+) cells were the major origin of IFN-gamma.
authors.affiliation: Department of Physiology, Osaka Medical College, Takatsuki, Osaka 569-8686, Japan.
authors.forename: Yukio
authors.initials: Y
authors.lastname: Yoneda
authors.forename: Ryuichiro
authors.initials: R
authors.lastname: Hirota
authors.forename: Junko
authors.initials: J
authors.lastname: Tashiro
authors.forename: Masashi
authors.initials: M
authors.lastname: Okada
authors.forename: Kanji
authors.initials: K
authors.lastname: Sakurai
authors.forename: Koutetsu
authors.initials: K
authors.lastname: Lee
authors.forename: Kouichi
authors.initials: K
authors.lastname: Ueda
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kubota
authors.forename: Ryotaro
authors.initials: R
authors.lastname: Yoshida
mesh_terms: D000505:Alopecia / Q000276:immunology / Q000473:pathology / Q000628:therapy; D000818:Animals; D001854:Bone Marrow Cells / Q000737:chemistry* / Q000378:metabolism; D005260:Female; D006197:Hair / Q000254:growth & development*; D018859:Hair Follicle / Q000737:chemistry; D007371:Interferon-gamma / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D016513:Mice, SCID; D012333:RNA, Messenger / Q000378:metabolism; D012867:Skin / Q000737:chemistry / Q000166:cytology*; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D012333:RNA, Messenger; D007371:Interferon-gamma