Association of hypoadiponectinemia with impaired vasoreactivity.
Endothelial dysfunction is a crucial feature in the evolution of atherosclerosis. Adiponectin is an adipocyte-specific plasma protein with antiatherogenic and antidiabetic properties. In the present study, we investigated the relation between adiponectin and endothelium-dependent vasodilation. We analyzed endothelial function in 202 hypertensive patients, including those who were not taking any medication. Forearm blood flow was measured by strain-gauge plethysmography. Plasma adiponectin level was highly correlated with the vasodilator response to reactive hyperemia in the total (r=0.257, P<0.001) and no-medication (r=0.296, P=0.026) groups but not with nitroglycerin-induced hyperemia, indicating that adiponectin affected endothelium-dependent vasodilation. Multiple regression analysis of data from all hypertensive patients revealed that plasma adiponectin level was independently correlated with the vasodilator response to reactive hyperemia. Vascular reactivity was also analyzed in aortic rings from adiponectin-knockout (KO) and wild-type (WT) mice. Adiponectin-KO mice showed obesity, hyperglycemia, and hypertension compared with WT mice after 4 weeks on an atherogenic diet. Endothelium-dependent vasodilation in response to acetylcholine was significantly reduced in adiponectin-KO mice compared with WT mice, although no significant difference was observed in endothelium-independent vasodilation in response to sodium nitroprusside. Our observations suggest that hypoadiponectinemia is associated with impaired endothelium-dependent vasorelaxation and that the measurement of plasma adiponectin level might be helpful as a marker of endothelial dysfunction.
Ouchi
Noriyuki
N
Department of Internal Medicine, Graduate School of Medicine, Osaka University, 2-2, Yamada-Oka, Suita, 565-0671 Osaka, Japan.
Ohishi
Mitsuru
M
Kihara
Shinji
S
Funahashi
Tohru
T
Nakamura
Tadashi
T
Nagaretani
Hiroyuki
H
Kumada
Masahiro
M
Ohashi
Koji
K
Okamoto
Yoshihisa
Y
Nishizawa
Hitoshi
H
Kishida
Ken
K
Maeda
Norikazu
N
Nagasawa
Azumi
A
Kobayashi
Hideki
H
Hiraoka
Hisatoyo
H
Komai
Norio
N
Kaibe
Masaharu
M
Rakugi
Hiromi
H
Ogihara
Toshio
T
Matsuzawa
Yuji
Y
D000109:Acetylcholine / Q000494:pharmacology; D052242:Adiponectin; D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects / Q000503:physiopathology; D001786:Blood Glucose / Q000378:metabolism; D001794:Blood Pressure / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D004036:Diet, Atherogenic; D004040:Dietary Carbohydrates / Q000008:administration & dosage; D004041:Dietary Fats / Q000008:administration & dosage; D004730:Endothelium, Vascular / Q000503:physiopathology; D005260:Female; D005542:Forearm / Q000098:blood supply; D006801:Humans; D006940:Hyperemia / Q000503:physiopathology; D006973:Hypertension / Q000097:blood / Q000503:physiopathology*; D066298:In Vitro Techniques; D036341:Intercellular Signaling Peptides and Proteins; D008055:Lipids / Q000097:blood; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008833:Microcirculation; D008875:Middle Aged; D009599:Nitroprusside / Q000494:pharmacology; D010991:Plethysmography / Q000379:methods; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012044:Regression Analysis; D017673:Sodium Chloride, Dietary / Q000008:administration & dosage; D014664:Vasodilation / Q000187:drug effects; D014665:Vasodilator Agents / Q000494:pharmacology
D052242:Adiponectin; D001786:Blood Glucose; D004040:Dietary Carbohydrates; D004041:Dietary Fats; D036341:Intercellular Signaling Peptides and Proteins; D008055:Lipids; D011506:Proteins; D017673:Sodium Chloride, Dietary; D014665:Vasodilator Agents; D009599:Nitroprusside; D000109:Acetylcholine