title: Expression of anthocyanins and proanthocyanidins after transformation of alfalfa with maize Lc.
abstract: Three anthocyanin regulatory genes of maize (Zea mays; Lc, B-Peru, and C1) were introduced into alfalfa (Medicago sativa) in a strategy designed to stimulate the flavonoid pathway and alter the composition of flavonoids produced in forage. Lc constructs included a full-length gene and a gene with a shortened 5'-untranslated region. Lc RNA was strongly expressed in Lc transgenic alfalfa foliage, but accumulation of red-purple anthocyanin was observed only under conditions of high light intensity or low temperature. These stress conditions induced chalcone synthase and flavanone 3-hydroxylase expression in Lc transgenic alfalfa foliage compared with non-transformed plants. Genotypes containing the Lc transgene construct with a full-length 5'-untranslated region responded more quickly to stress conditions and with a more extreme phenotype. High-performance liquid chromatography analysis of field-grown tissue indicated that flavone content was reduced in forage of the Lc transgenic plants. Leucocyanidin reductase, the enzyme that controls entry of metabolites into the proanthocyanidin pathway, was activated both in foliage and in developing seeds of the Lc transgenic alfalfa genotypes. Proanthocyanidin polymer was accumulated in the forage, but (+)-catechin monomers were not detected. B-Peru transgenic and C1 transgenic populations displayed no visible phenotypic changes, although these transgenes were expressed at detectable levels. These results support the emerging picture of Lc transgene-specific patterns of expression in different recipient species. These results demonstrate that proanthocyanidin biosynthesis can be stimulated in alfalfa forage using an myc-like transgene, and they pave the way for the development of high quality, bloat-safe cultivars with ruminal protein bypass.
authors.affiliation: Saskatoon Research Centre, Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, Saskatchewan, Canada S7N 0X2.
authors.forename: Heather
authors.initials: H
authors.lastname: Ray
authors.forename: Min
authors.initials: M
authors.lastname: Yu
authors.forename: Patricia
authors.initials: P
authors.lastname: Auser
authors.forename: Laureen
authors.initials: L
authors.lastname: Blahut-Beatty
authors.forename: Brian
authors.initials: B
authors.lastname: McKersie
authors.forename: Steve
authors.initials: S
authors.lastname: Bowley
authors.forename: Neil
authors.initials: N
authors.lastname: Westcott
authors.forename: Bruce
authors.initials: B
authors.lastname: Coulman
authors.forename: Alan
authors.initials: A
authors.lastname: Lloyd
authors.forename: Margaret Y
authors.initials: MY
authors.lastname: Gruber
mesh_terms: D000872:Anthocyanins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D005419:Flavonoids / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D005838:Genotype; D000455:Medicago sativa / Q000235:genetics* / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D044945:Proanthocyanidins; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D019076:Transgenes / Q000235:genetics; D003313:Zea mays / Q000235:genetics*
chemical_list: D000872:Anthocyanins; D005419:Flavonoids; D010088:Oxidoreductases; D010940:Plant Proteins; D044945:Proanthocyanidins; D014157:Transcription Factors; C083017:R-S protein, Zea mays; C013221:proanthocyanidin; C001532:leucocyanidin