title: Contribution of ionotropic glutamate receptors to acute amphetamine-stimulated preproenkephalin mRNA expression in the rat striatum in vivo.
abstract: The present study evaluated the possible role of ionotropic glutamate receptors in mediating the inducible preproenkephalin (PPE) mRNA expression in the rat striatum in response to systemic administration of a psychostimulant amphetamine. A single injection of amphetamine (5 mg/kg, i.p.) increased PPE mRNA levels in the dorsal striatum as revealed via quantitative in situ hybridization. Pretreatment with the NMDA receptor antagonist, MK801 (0.5 mg/kg, i.p.) or 3-((R)-2-carboxypiperazin-4-yl)-propyl-1-phosphonic acid (10 mg/kg, i.p.), attenuated the amphetamine-induced PPE mRNA expression. A similar attenuation of the amphetamine-induced PPE expression, although to a lesser extent, was also observed following pretreatment with a kainate/AMPA receptor antagonist, 6,7-dinitroquinoxaline-2,3-dione (100-200 mg/kg, i.p.). Three antagonists alone had no significant effect on basal levels of PPE mRNAs in the dorsal striatum. These data indicate that both NMDA and kainate/AMPA receptors are involved in coupling extracellular signals evoked by acute stimulant exposure to PPE mRNA expression in striatal neurons.
authors.affiliation: Division of Pharmacology, School of Pharmacy, University of Missouri-Kansas City, 2411 Holmes Street, Kansas City, MO 64108, USA.
authors.forename: Limin
authors.initials: L
authors.lastname: Mao
authors.forename: John Q
authors.initials: JQ
authors.lastname: Wang
mesh_terms: D000661:Amphetamine / Q000494:pharmacology*; D000818:Animals; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism; D004745:Enkephalins / Q000096:biosynthesis* / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D008297:Male; D011498:Protein Precursors / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D018092:Kainic Acid Receptors / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D000661:Amphetamine; D004745:Enkephalins; D011498:Protein Precursors; D012333:RNA, Messenger; D018092:Kainic Acid Receptors; C033660:preproenkephalin