title: Fourier transform infrared spectroscopy for screening and quantifying production of PHAs by Pseudomonas grown on sodium octanoate.
abstract: Poly(hydroxyalkanoates) PHAs are synthesized by many bacteria as inclusion bodies and their biodegradability and structural diversity have been studied with a view to their potential application as biodegradable materials. A method based on FT-IR was developed to carry out rapid qualitative and quantitative analysis of PHAs in Pseudomonas, when they were grown on sodium octanoate. Using absorbance of the ester band of PHAs, a rapid method was reported to distinguish PHB and PHO and to determine polymer content in intact bacteria. Relative areas in which the C=O area was normalized to the area of the peak representing the amid group (1656 cm(-1)) characteristic of bacteria were calibrated to the polymer content which was determined after solvent extraction. Polymer contents vary from 0% to 53% and depend on the nature of the bacteria. Among 27 strains of Pseudomonas belonging to the rRNA homology group I, a very low amount of bacteria were able to produce PHB. The majority of strains were able to produce a copolymer, PHO, in which the major constituent unit is 3-hydroxyoctanoate. The FT-IR results were further confirmed by gas chromatography analysis after methanolysis of polymer, but FT-IR method requires less preparation of sample than gas chromatography and it is very useful for screening a large variety of Pseudomonas.
authors.affiliation: Laboratoire de Recherches sur les Polymères, UMR 7581, 2 à 8 rue Henri Dunant, 94 320 Thiais, France.
authors.forename: Solo
authors.initials: S
authors.lastname: Randriamahefa
authors.forename: Estelle
authors.initials: E
authors.lastname: Renard
authors.forename: Philippe
authors.initials: P
authors.lastname: Guérin
authors.forename: Valérie
authors.initials: V
authors.lastname: Langlois
mesh_terms: D002210:Caprylates / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D011091:Polyesters / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D011549:Pseudomonas / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D000094122:Polyhydroxybutyrates
chemical_list: D002210:Caprylates; D006885:Hydroxybutyrates; D011091:Polyesters; C488798:poly(3-hydroxycaproate); C488799:poly(3-hydroxydecanoate); C088151:poly(3-hydroxyoctanoic acid); C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C031492:octanoic acid