Synthesis of novel cationic lipids having polyamidoamine dendrons and their transfection activity.
We designed a novel type of cationic lipid, lipids with a cationic polar group in the polyamidoamine dendron, because these dendron-bearing lipids are expected to form complexes with plasmid DNA and achieve efficient transfection of cells by synergy of endosome buffering and membrane fusion with the endosome, both of which are useful for the promotion of the transfer of plasmid DNA from endosome to cytosol. Four kinds of lipids with polyamidoamine dendrons of first to fourth generations, DL-G1, DL-G2, DL-G3, and DL-G4, were synthesized. The lipid with a dendron of a higher generation exhibited greater ability to form lipoplexes with plasmid DNA, as estimated by agarose gel electrophoresis. While the DL-G1 lipoplex did not transfect CV1 cells, the lipoplexes containing the DL-G2, DL-G3, or DL-G4 could induce transfection of the cells, and their activity was elevated with increasing generation of the dendron. Addition of dioleoylphosphatidylethanolamine (DOPE), which is known to increase fusion ability of a lipid membrane, into the lipoplexes greatly enhanced their transfection activity. In addition, the comparison with DC-Chol-containing lipoplex, which is widely used as a nonviral vector, showed that the DL-G3-DOPE lipoplex exhibits more efficient transfections. These findings imply that these dendron-bearing lipids may form the basis for a novel family of cationic lipids for efficient gene delivery.
Takahashi
Toshinari
T
Department of Applied Materials Science, Graduate School of Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka 599-8531, Japan.
Kono
Kenji
K
Itoh
Toshihide
T
Emi
Nobuhiko
N
Takagishi
Toru
T
D000818:Animals; D002412:Cations / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D002460:Cell Line; D002738:Chloroquine / Q000494:pharmacology; D004247:DNA / Q000737:chemistry / Q000187:drug effects / Q000235:genetics*; D005822:Genetic Vectors / Q000737:chemistry / Q000235:genetics; D050356:Lipid Metabolism; D008055:Lipids / Q000138:chemical synthesis* / Q000737:chemistry; D008156:Luciferases / Q000187:drug effects / Q000378:metabolism; D008956:Models, Chemical; D015394:Molecular Structure; D010714:Phosphatidylethanolamines / Q000737:chemistry; D011073:Polyamines / Q000737:chemistry*; D014162:Transfection / Q000379:methods*
C410262:1,2-dioleoyl-glycero-3-phosphatidyl ethanolamine; D002412:Cations; D008055:Lipids; D010714:Phosphatidylethanolamines; D011073:Polyamines; D002738:Chloroquine; D004247:DNA; D008156:Luciferases