title: [Transplantation of autologous peripheral blood stem cells for the treatment of lower limb arteriosclerosis obliterans].
abstract: OBJECTIVE
  To evaluate the clinical efficacy of mobilized autologous peripheral blood stem cells (PBSC) transplantation in a 48 years old patient with lower limb arteriosclerosis obliterans (ASO).
  
  
  METHODS
  rhG-CSF 600 micro g/d for 5 days to mobilize stem cells. On the fifth day, PBSC were collected with a Version 4 blood-cells separator. Three hours late, the PBSC were intramuscularly injected into the ischemic areas of the two lower limbs (3 x 10(9) cells per limb). The clinical and laboratory findings were monitored every week for 3 months. Forty-four days after the implantation, left lower limb with severe ASO was given an additional implantation of the same number of cells as the first time.
  
  
  RESULTS
  The peripheral blood CD(34)(+) cells were increased from 0.18% to 0.75% after 5 days of rhG-CSF mobilization. Three months after the first stem cell transplantation, severe pain lameness, local cool-feeling and ulcer were improved, and ABI increased from 0.49, 0.69 to 0.50, 0.85, the amplitude of blood flow and laser Doppler blood perfusion were also significantly improved (P < 0.01). At the same time, digital subtraction angiographic scores for new collateral vessel formation were showed as + 3(rich). No related complication or adverse effect were observed during the 3-month observation.
  
  
  CONCLUSIONS
  Transplantation of mobilized autologous PBSC might be a simple, safe, and effective method for the treatment of ASO.
authors.affiliation: Institute of Hematology & Blood Diseases Hospital, National Research Center of Stem Cell Engineering & Technology, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
authors.forename: Ping-ping
authors.initials: PP
authors.lastname: Huang
authors.forename: Shang-zhu
authors.initials: SZ
authors.lastname: Li
authors.forename: Ming-zhe
authors.initials: MZ
authors.lastname: Han
authors.forename: Zhi-jian
authors.initials: ZJ
authors.lastname: Xiao
authors.forename: Ren-chi
authors.initials: RC
authors.lastname: Yang
authors.forename: Lu-gui
authors.initials: LG
authors.lastname: Qiu
authors.forename: Guan-qing
authors.initials: GQ
authors.lastname: Qian
authors.forename: Ren-min
authors.initials: RM
authors.lastname: Fu
authors.forename: Ya-li
authors.initials: YL
authors.lastname: Zheng
authors.forename: Chun-hua
authors.initials: CH
authors.lastname: Liu
authors.forename: Xiu-qiang
authors.initials: XQ
authors.lastname: Xu
authors.forename: Jun-fan
authors.initials: JF
authors.lastname: Li
authors.forename: Zhong-chao
authors.initials: ZC
authors.lastname: Han
mesh_terms: D001162:Arteriosclerosis Obliterans / Q000628:therapy*; D006801:Humans; D035002:Lower Extremity; D008297:Male; D008875:Middle Aged; D036102:Peripheral Blood Stem Cell Transplantation; D014182:Transplantation, Autologous