title: Skin tests and conjunctival and bronchial challenges with extracts of Blomia tropicalis and Dermatophagoides pteronyssinus in patients with allergic asthma and/or rhinoconjunctivitis.
abstract: BACKGROUND
  Blomia tropicalis and Dermatophagoides pteronyssinus are important mite species in Tenerife, Canary Islands, Spain. Several studies have demonstrated a variable degree of allergenic cross-reactivity in vitro. However, only a few have addressed their allergenic cross-reactivity using challenge tests.
  
  
  OBJECTIVE
  The objective of this study was to conduct conjunctival and bronchial challenge tests with B. tropicalis and D. pteronyssinus extracts in a group of 42 patients with allergic asthma and/or rhinoconjunctivitis sensitised to house dust mites (31 females, 11 males; mean age 21.7 +/- 7.02 years).
  
  
  METHODS
  Prick tests using standardised extracts and specific IgE determinations using the CAP system were performed. Bronchial and/or conjunctival challenges were conducted using freeze-dried extracts of both mite species. A patient was considered sensitive to a mite species if she/he had a positive prick and/or CAP test result. A total of 32 conjunctival and 15 bronchial challenges were performed with both mite species.
  
  
  RESULTS
  Prick tests were positive to B. tropicalis in 23 patients (54.7%) and to D. pteronyssinus in 41 (97.6%). One patient (2.4%) was exclusively sensitive to B. tropicalis. The CAP test was positive for B. tropicalis in 28 patients and for D. pteronyssinus in 41. Conjunctival challenges to B. tropicalis were positive in 20 patients (18 sensitised and 2 non-sensitised) and negative in 12 (5 sensitised and 7 non-sensitised patients). Conjunctival challenges with D. pteronyssinus were positive in all 31 D. pteronyssinus-sensitised patients who underwent conjunctival challenges. Bronchial challenges with B. tropicalis were positive in 9 sensitised patients and negative in 6 patients (2 sensitised and 4 non-sensitised). Bronchial challenges with D. pteronyssinus were positive in all patients except 1, who only reacted to B. tropicalis.
  
  
  CONCLUSIONS
  Allergens of the mite species B. tropicalis induce positive conjunctival and bronchial challenges in B. tropicalis-sensitised individuals. Our results suggest that although there is a low to moderate degree of in vivo cross-reactivity between B. tropicalis and D. pteronyssinus, B. tropicalis seems to be a relevant source of allergens in areas where patients are exposed.
authors.affiliation: Servicio Alergia, Hospital Universitario Nuestra Señora de la Candelaria, Tenerife, Canary Islands, Spain. jgarciar@meditex.es
authors.forename: J C
authors.initials: JC
authors.lastname: García Robaina
authors.forename: I
authors.initials: I
authors.lastname: Sánchez Machín
authors.forename: E
authors.initials: E
authors.lastname: Fernández-Caldas
authors.forename: V
authors.initials: V
authors.lastname: Iraola Calvo
authors.forename: C
authors.initials: C
authors.lastname: Vázquez Moncholi
authors.forename: C
authors.initials: C
authors.lastname: Bonnet Moreno
authors.forename: F
authors.initials: F
authors.lastname: de la Torre Morín
mesh_terms: D000053:Acari / Q000276:immunology*; D000293:Adolescent; D000328:Adult; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000009:adverse effects / Q000276:immunology*; D001249:Asthma / Q000276:immunology*; D001985:Bronchial Provocation Tests; D002648:Child; D003228:Conjunctiva / Q000276:immunology; D003233:Conjunctivitis, Allergic / Q000209:etiology / Q000276:immunology*; D003429:Cross Reactions; D040002:Dermatophagoides pteronyssinus / Q000276:immunology*; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis; D008297:Male; D039981:Pyroglyphidae / Q000276:immunology; D012221:Rhinitis, Allergic, Perennial / Q000209:etiology / Q000276:immunology*; D012882:Skin Tests; D013030:Spain
chemical_list: D039741:Antigens, Dermatophagoides; D007073:Immunoglobulin E