Augmentation of B7 expression by herpes simplex virus antigen.
B7 costimulation plays a major role in herpes simplex virus (HSV) immunity. Effects of immunoregulatory cytokines (interferon-gamma [IFN-gamma] and interleukin-10 [IL-10]) and HSV antigens on B7-1 (CD80) and B7-2 (CD86) expression on monocytes during HSV-2 infection were investigated using flow cytometry. Our results demonstrate that HSV-2 infection effects the expression of B7 isoforms (B7-1 and B7-2) on monocytes in two ways, with opposing outcomes. It abrogates the IFN-gamma upregulation of B7-1 and B7-2 and also augments expression of B7-1 and B7-2 on monocytes through an IFN-gamma independent mechanism. The clinical significance of these opposing effects may be related to the pathogenesis of recurrent HSV disease in immunocompetent hosts.
Singh
Rekha
R
Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Ontario, Canada. singh_rek@yahoo.com
Kumar
Ashok
A
Diaz-Mitoma
Francisco
F
D015703:Antigens, CD / Q000276:immunology* / Q000378:metabolism*; D000956:Antigens, Viral / Q000276:immunology*; D018122:B7-1 Antigen / Q000276:immunology / Q000378:metabolism*; D051940:B7-2 Antigen; D002478:Cells, Cultured; D005434:Flow Cytometry; D006558:Herpes Genitalis / Q000276:immunology* / Q000821:virology; D018259:Herpesvirus 1, Human / Q000276:immunology; D018258:Herpesvirus 2, Human / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology*; D016753:Interleukin-10 / Q000276:immunology; D008562:Membrane Glycoproteins / Q000276:immunology* / Q000378:metabolism*; D018448:Models, Immunological; D009000:Monocytes / Q000276:immunology* / Q000821:virology; D013601:T-Lymphocytes / Q000276:immunology; D015854:Up-Regulation
D015703:Antigens, CD; D000956:Antigens, Viral; D018122:B7-1 Antigen; D051940:B7-2 Antigen; C497964:CD86 protein, human; D008562:Membrane Glycoproteins; D016753:Interleukin-10; D007371:Interferon-gamma