Attenuated total reflectance Fourier transform mid-infrared spectroscopic quantification of sorbitol and sorbose during a Gluconobacter biotransformation process.
Mid-infrared spectroscopy (MIRS) was used to simultaneously detect and predict concentrations of D-sorbitol and L-sorbose during a Gluconobacter suboxydans biotransformation. Quantitative models for both these compounds were developed for the entire time-course of the process and validated externally using samples not included in the original modelling exercise, giving standard errors of prediction of 3.29 and 3.3% for sorbitol and sorbose, respectively, and a correlation coefficient close to 1.
Macauley-Patrick
Sue
S
Strathclyde Fermentation Centre, Department of Bioscience, University of Strathclyde, 204 George St., Glasgow G1 1XW, UK.
Arnold
S Alison
SA
McCarthy
Brendan
B
Harvey
Linda M
LM
McNeil
Brian
B
D019149:Bioreactors / Q000382:microbiology; D001711:Biotransformation; D020576:Gluconobacter / Q000737:chemistry / Q000378:metabolism*; D013012:Sorbitol / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D013013:Sorbose / Q000032:analysis* / Q000096:biosynthesis / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D013012:Sorbitol; D013013:Sorbose