Real-time PCR method to detect Enterococcus faecalis in water.
A 16S rDNA real-time PCR method was developed to detect Enterococcus faecalis in water samples. The dynamic range for cell detection spanned five logs and the detection limit was determined to be 6 cfu/reaction. The assay was capable of detecting E. faecalis cells added to biofilms from a simulator of a water distribution system and in freshwater samples. Nucleic acid extraction was not required, permitting the detection of E. faecalis cells in less than 3 h.
Santo Domingo
Jorge W
JW
U.S. Environmental Protection Agency, National Exposure Research Laboratory, Microbial Exposure Research Branch, 26 W Martin Luther King Dr, Cincinnati, OH 45268, USA. santodomingo.jorge@epa.gov
Siefring
Shawn C
SC
Haugland
Richard A
RA
D004269:DNA, Bacterial / Q000032:analysis; D013293:Enterococcus faecalis / Q000145:classification / Q000302:isolation & purification*; D004784:Environmental Monitoring / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014867:Water / Q000032:analysis*; D014871:Water Microbiology; D014876:Water Pollution / Q000032:analysis*
D004269:DNA, Bacterial; D014867:Water