Equilibrium protein folding-unfolding process involving multiple intermediates.
Mathematical models for the protein folding-unfolding process involving multiple intermediates have been derived. Computer fitting of the experimental data to this model generates various thermodynamic parameters for the folding-unfolding process. In this way, the complex folding-unfolding process of the multi-domain proteins can be analysed in a quantitative way. The application of the folding-unfolding model involving seven stages in human placental alkaline phosphatase is described.
Hung
Hui-Chih
HC
Department of Life Science, National Chung-Hsing University, Taichung, Taiwan.
Chen
Yu-Hou
YH
Liu
Guang-Yaw
GY
Lee
Hwei-Jen
HJ
Chang
Gu-Gang
GG
D000469:Alkaline Phosphatase / Q000737:chemistry*; D003198:Computer Simulation; D005260:Female; D006801:Humans; D007700:Kinetics; D008956:Models, Chemical; D010920:Placenta / Q000201:enzymology; D017510:Protein Folding
D000469:Alkaline Phosphatase