Protective effect of polypeptide from Chlamys farreri on mitochondria in human dermal fibroblasts irradiated by ultraviolet B.
OBJECTIVE
To study the effect of polypeptide from Chlamys farreri (PCF) on mitochondria of human dermal fibroblasts irradiated by ultraviolet B (UVB) in vitro.


METHODS
Malondialdehyde (MDA) and antioxidant enzymes including superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) were determined by biochemical methods. Mitochondrial transmembrane potential was measured by flow cytometry. Ultrastructure of fibroblasts was observed with transmission electron microscope.


RESULTS
UVB (1.176 x 10(-4) J/cm(2)) induced mitochondria damage in dermal fibroblast and PCF (0.25%-1%) reduced the damage in a concentration-dependent manner. Furthermore, PCF also concentration-dependently maintained the stability of mitochondrial transmembrane potential. PCF was able to reduce the MDA formation caused by UVB, meanwhile increased the activities of SOD and GSH-PX. The differences among the PCF groups and UVB model group were significant (P<0.05, P<0.01).


CONCLUSIONS
The UVB-induced mitochondria damage was alleviated by PCF in human dermal fibroblasts.
Wang
Chun-Bo
CB
Department of Pharmacology, Medical College, Qingdao University, Qingdao 266021, China. liujg666@yahoo.com.cn
Ding
Bo-Xiao
BX
Guo
Shen-Bo
SB
Wang
Yu-Zhen
YZ
Han
Yan-Tao
YT
Wang
Yue-Jun
YJ
D000293:Adolescent; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002478:Cells, Cultured; D020405:Dermis / Q000166:cytology; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects* / Q000528:radiation effects / Q000648:ultrastructure; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D008315:Malondialdehyde / Q000378:metabolism; D008419:Materia Medica / Q000302:isolation & purification / Q000494:pharmacology*; D008564:Membrane Potentials; D008928:Mitochondria / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects; D008974:Mollusca / Q000737:chemistry*; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D014466:Ultraviolet Rays
D000975:Antioxidants; D008419:Materia Medica; D010455:Peptides; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase