title: Development of a species-specific recA-based PCR test for Burkholderia fungorum.
abstract: The genus Burkholderia comprises over 28 species and species-specific, recA-based polymerase chain reaction (PCR) tests are available for several species, but not for some soil-inhabiting species including B. fungorum. Previous analysis of several novel rhizospheric, environmental isolates belonging to the B. cepacia complex suggested they may be closely related to B. fungorum. To discover any relationship between these isolates and B. fungorum we set out to clone and sequence a portion of the B. fungorum recA gene in order to design species-specific primer pairs for use in a recA-based PCR assay. Using a similar procedure we extended the recA-based PCR assay to identify B. sacchari and B. caledonica, two additional soil-inhabiting Burkholderia spp.
authors.affiliation: Department of Biological Sciences, Imperial College London, Sir Alexander Fleming Building, Imperial College Road, London SW7 2AZ, UK.
authors.forename: Chun-Han
authors.initials: CH
authors.lastname: Chan
authors.forename: D E
authors.initials: DE
authors.lastname: Stead
authors.forename: R H A
authors.initials: RH
authors.lastname: Coutts
mesh_terms: D019117:Burkholderia / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D011938:Rec A Recombinases / Q000235:genetics*; D012988:Soil Microbiology; D013045:Species Specificity
chemical_list: D011938:Rec A Recombinases