Inhalation exposure to methylene chloride does not induce systemic immunotoxicity in rats.
Methylene chloride (dichloromethane) is used in a variety of industrial applications. To date, there has been no formal assessment of immunotoxicity attributed to methylene chloride. Studies were undertaken to examine whether methylene chloride has any potential to influence the integrity of immune function. For this purpose, Sprague-Dawley rats of both genders were exposed by inhalation to a single high dose (5000 ppm) of methylene chloride for 6 h/d, 5 d/wk for 28 d. This was considered the relevant route of administration, as not only is inhalation a primary route for human exposure to methylene chloride, but, also, the chemical is absorbed rapidly via the lungs. Under these conditions of exposure, methylene chloride failed to influence absolute or relative thymus weights in either gender and produced a significant reduction in relative, but not absolute, spleen weight in female rats only. Immunocompetence was measured as a function of the ability of treated animals to mount immunoglobulin M (IgM) antibody responses to sheep red blood cells (SRBC) as determined by enzyme-linked immunosorbent assay (ELISA). Exposure to methylene chloride did not affect antibody production. Evidence indicates that under these conditions of exposure, methylene chloride did not compromise immune function.
Warbrick
E V
EV
Syngenta Central Toxicology Laboratory, Alderley Park, Macclesfield, Cheshire, United Kingdom.
Kilgour
J D
JD
Dearman
R J
RJ
Kimber
I
I
Dugard
P H
PH
D000280:Administration, Inhalation; D000818:Animals; D000917:Antibody Formation; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007107:Immune System / Q000187:drug effects*; D007075:Immunoglobulin M / Q000032:analysis*; D008297:Male; D008752:Methylene Chloride / Q000009:adverse effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000033:anatomy & histology / Q000473:pathology; D013950:Thymus Gland / Q000033:anatomy & histology / Q000473:pathology
D007075:Immunoglobulin M; D008752:Methylene Chloride