Lipid and oxylipin profiles during aging and sprout development in potato tubers (Solanum tuberosum L.).
Potato tubers (Solanum tuberosum L. cv Bintje) were stored at 20 degrees C for 210 days without desprouting to study the lipoxygenase pathway during aging. After 15 days of storage, potato tubers sprouted, while after 45-60 days, apical dominance was lost and multiple sprouts developed. Analysis of the fatty acid hydroperoxides (HPOs) revealed that 9-S-hydroperoxide of linoleic acid (9-HPOD) was the main oxylipin formed. Between 45 and 60 days of storage, increases in the levels of 9-HPOD and colneleic acid were observed. Analysis of phospholipids and galactolipids by electrospray ionisation tandem mass spectrometry (ESI-MS/MS) showed that a decrease in the levels of phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), digalactosyldiacylglycerol (DGDG), and monogalactosyldiacylglycerol (MGDG) occurred between 0 and 45 days of aging. The decrease in the amount of linoleic acid in complex lipids correlates well with the amount of 9-HPOD and colneleic acid produced.
Fauconnier
Marie Laure
ML
Unité de Chimie Générale et Organique, Faculté Universitaire des Sciences Agronomiques de Gembloux, Passage des Déportés 2, B-5030, Gembloux, Belgium. fauconnier.ml@fsagx.ac.be
Welti
Ruth
R
Blée
Elizabeth
E
Marlier
Michel
M
D004987:Ethers / Q000032:analysis; D005229:Fatty Acids, Monounsaturated / Q000032:analysis; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D038983:Galactolipids / Q000032:analysis; D050356:Lipid Metabolism; D008054:Lipid Peroxides / Q000032:analysis / Q000378:metabolism*; D008055:Lipids / Q000032:analysis; D008084:Lipoxygenase / Q000378:metabolism; D008563:Membrane Lipids / Q000378:metabolism; D010743:Phospholipids; D011198:Solanum tuberosum / Q000254:growth & development / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D013997:Time Factors
D004987:Ethers; D005229:Fatty Acids, Monounsaturated; D005231:Fatty Acids, Unsaturated; D038983:Galactolipids; D008054:Lipid Peroxides; D008055:Lipids; D008563:Membrane Lipids; D010743:Phospholipids; C118818:colnelenic acid; C002946:colneleic acid; D008084:Lipoxygenase