[Gene expression of extracellular-signal regulated protein kinase 1/2 and their MAPKKs in hypertrophic scars].
OBJECTIVE
To explore the change of extracellular-signal regulated protein kinase 1/2 (ERK1/2) and their mitogen activated protein kinase kinases(MAPKKs) gene expression in normal skins versus hypertrophic scars underlying their biological significances.


METHODS
After total RNAs were isolated from 8 specimens of hypertrophic scars and 8 specimens of normal skins, and then were purified to mRNAs, the gene expression of ERK1/2 and MEK1/2 were examined with reverse transcription-polymerase chain reaction (RT-PCR).


RESULTS
In normal skins, the intensity of gene expression of ERK1/2 and MEK1/2 were weak. In hypertrophic scar, the expression intensities of these 4 genes were 1.1 times, 1.2 times, 2.2 times and 2.5 times of normal skins, respectively. The expression of these 4 genes was significantly elevated in comparison with normal skins.


CONCLUSIONS
The activation of MEK1/2, ERK1/2 gene expression in hypertrophic scars versus in normal skins might be one of the mechanisms controlling the formation of hypertrophic scars.
Chen
Wei
W
304th Hospital of People's Liberation Army, Beijing 100037, China.
Fu
Xiao-bing
XB
Sun
Xiao-qing
XQ
Sun
Tong-zhu
TZ
Zhao
Zhi-li
ZL
Zhou
Gang
G
Yan
Yin-hui
YH
Sheng
Zhi-yong
ZY
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D017439:Cicatrix, Hypertrophic / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005260:Female; D015870:Gene Expression; D006801:Humans; D048369:MAP Kinase Kinase 1 / Q000235:genetics / Q000378:metabolism*; D048370:MAP Kinase Kinase 2 / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D019950:Mitogen-Activated Protein Kinase 1 / Q000235:genetics / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D055815:Young Adult
C482128:MAP2K2 protein, human; C535150:MAPK1 protein, human; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D048369:MAP Kinase Kinase 1; D048370:MAP Kinase Kinase 2