title: [Transfection of chk1/2 antisense oligonucleotide to HL-60 cell line increases the apoptotic sensitivity to irradiation].
abstract: OBJECTIVE
  To block signal transduction of cell cycle checkpoints by antisense blocking of chk1/2 gene to increase the radiation sensitivity of HL-60 cell line.
  
  
  METHODS
  To transfect the HL-60 cell with chk1/2 antisense and sense chain alone and in combination, expose the cells to irradiation at 24 h after the transfection, the chk1 protein change was assayed by Western blot and the cell cycles and annexin V apoptosis rates by FCM.
  
  
  RESULTS
  The irradiated apoptosis sensitivity was increased by antisense blocking of chk1 gene in HL-60 cell line, the apoptotic rate was 26.31% being significantly higher than that of the sense blocking (10.34%) (P < 0.05), Furthermore, the G(2)/M phase blocking phenomenon decreased and a synergic effect was observed in antisense blocking both the chk1 and chk2 genes.
  
  
  CONCLUSIONS
  Antisense blocking of chk1/chk2 could increase the apoptotic sensitivity to irradiation.
authors.affiliation: Hematology Department of Tongji Hospital, Tongji College of Huazhong Science & Technology University, Wuhan 430030, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Tang
authors.forename: Wen-li
authors.initials: WL
authors.lastname: Liu
authors.forename: Jian-feng
authors.initials: JF
authors.lastname: Zhou
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Wan
authors.forename: Qing-lei
authors.initials: QL
authors.lastname: Gao
authors.forename: Jian-hong
authors.initials: JH
authors.lastname: Wu
mesh_terms: D017209:Apoptosis / Q000235:genetics / Q000528:radiation effects; D002453:Cell Cycle / Q000528:radiation effects; D000071877:Checkpoint Kinase 1; D064447:Checkpoint Kinase 2; D018922:HL-60 Cells; D006801:Humans; D016376:Oligonucleotides, Antisense / Q000235:genetics*; D011494:Protein Kinases / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D011836:Radiation Tolerance; D014162:Transfection
chemical_list: D016376:Oligonucleotides, Antisense; D011494:Protein Kinases; D064447:Checkpoint Kinase 2; C000605867:CHEK1 protein, human; C578634:CHEK2 protein, human; D000071877:Checkpoint Kinase 1; D017346:Protein Serine-Threonine Kinases