p65 and c-erbB2 genes expression in breast tumors: comparison with some histological typing, grading and clinical staging.
Using PCR technique we have analyzed p65 and c-erbB2 genes expression in 47 frozen tissue slides taken from patients diagnosed as ductal and lobular breast cancer, classified as G3, and in a limited panel of proliferative breast disease cases. Expression of p65 was generally connected with small tumor size and with absence of metastases in regional lymph nodes. We have found interdependence between p65 gene expression and negative states of lymph nodes. On the contrary, c-erbB2 expression was observed in patients with large tumors and with metastases to the regional lymph nodes. Between both genes (p65 and c-erbB2) opposite interdependence was found. No statistical dependence between estrogen/progesterone receptor levels and p65 or c-erbB2 expression were noticed. The presence of p65 expression appeared in the group of proliferating breast disease cases which were connected with higher risk of breast cancer. Lack of p65 expression accompanied cases which were classified as fibroadenoma.
Balcerczak
E
E
Dept. of Pharmaceutical Biochemistry, Molecular Biology Laboratory, Faculty of Pharmacy, Medical University, Lodz, Poland.
Mirowski
M
M
Jesionek-Kupnicka
D
D
Bartkowiak
J
J
Kubiak
R
R
Wierzbicki
R
R
D014408:Biomarkers, Tumor; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology*; D002352:Carrier Proteins / Q000096:biosynthesis*; D002455:Cell Division; D005260:Female; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008207:Lymphatic Metastasis; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D009367:Neoplasm Staging; D012313:RNA / Q000378:metabolism; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis*; D011960:Receptors, Estrogen / Q000096:biosynthesis; D011980:Receptors, Progesterone / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D014408:Biomarkers, Tumor; D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D009363:Neoplasm Proteins; D012313:RNA; D018719:Erb-b2 Receptor Tyrosine Kinases; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; C484020:HSAJ2425 protein, human