title: Fur mutants of Pseudomonas aeruginosa PAO1: phenotypic characterization in terms of iron-dependent pattern of deregulation of pyoverdin synthesis.
abstract: Fur mutants (Mn(r)) of Pseudomonas aeruginosa Fe10 (FF13 and FF28) and PAO1 (FPA12) were evaluated for the pattern of deregulation of pyoverdin synthesis in iron-replete medium with Casamino acids [CAM(Fe)] or succinate [SM(Fe)]. With respect to siderophore synthesis, we found in CAM(Fe) medium two Fur phenotypes: FurA (full deregulation, FF13) and FurB (partial deregulation, FF28 and FPA12). Fur mutants compared to parental strains grew with slower specific growth rates on SM(0) and CAM(0) media in a stirred bioreactor. Fur mutants grew in SM(0) with mu about half of that in CAM(0).
authors.affiliation: Laboratory of Enzyme Technology, Institute of Microbiology, Academy of Sciences of the Czech Republic, 14220 Prague 4, Czech Republic.
authors.forename: Andrea
authors.initials: A
authors.lastname: Johnová
authors.forename: Marie
authors.initials: M
authors.lastname: Dobisová
authors.forename: M A
authors.initials: MA
authors.lastname: Abdallah
authors.forename: Pavel
authors.initials: P
authors.lastname: Kyslík
mesh_terms: D000596:Amino Acids / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D007501:Iron / Q000494:pharmacology*; D016296:Mutagenesis; D009154:Mutation; D009842:Oligopeptides; D010641:Phenotype; D010860:Pigments, Biological / Q000096:biosynthesis*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D017262:Siderophores / Q000096:biosynthesis; D019802:Succinic Acid / Q000494:pharmacology
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; D009842:Oligopeptides; D010860:Pigments, Biological; D012097:Repressor Proteins; D017262:Siderophores; C017721:casamino acids; C068477:ferric uptake regulating proteins, bacterial; C042453:pyoverdin; D019802:Succinic Acid; D007501:Iron