Determination of bupivacaine and metabolites in rat urine using capillary electrophoresis with mass spectrometric detection.
A method using capillary electrophoresis-mass spectrometry (CE-MS) was developed for the structural elucidation of bupivacaine and metabolites in rat urine. Prior to CE-MS analysis, solid-phase extraction (SPE) was used for sample cleanup and preconcentration purposes. Exact mass and tandem mass spectrometric (MS/MS) experiments were performed to obtain structural information about the unknown metabolites. Two instruments with different mass analyzers were used for mass spectrometric detection. A quadrupole time-of-flight (Q-TOF) and a magnetic sector hybrid instrument were coupled to CE and used for the analysis of urine extracts. Hydroxybupivacaine as well as five other isomerically different metabolites were detected including methoxylated bupivacaine.
Krisko
Ryan M
RM
Department of Chemistry, University of Kansas, Lawrence, KS, USA.
Schieferecke
Mark A
MA
Williams
Todd D
TD
Lunte
Craig E
CE
D000818:Animals; D002045:Bupivacaine / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry / Q000378:metabolism / Q000652:urine*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D015394:Molecular Structure; D051381:Rats; D017207:Rats, Sprague-Dawley; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods* / Q000592:standards
C121551:3-hydroxybupivacaine; C051879:4'-hydroxybupivacaine; C025149:2',6'-pipecoloxylidide; D002045:Bupivacaine