title: Typing of Listeria monocytogenes isolates by random amplification of polymorphic DNA.
abstract: OBJECTIVE
  Listeria monocytogenes is an important food-borne pathogen causing meningitis and septicaemia in newborns and immunocompromised persons, abortion and preterm labour in pregnant women. Though various methods are available for typing L. monocytogenes, RAPD analysis has been used for epidemiological purposes in developed countries due to its greater discriminating ability. However, as there are no published reports from India on the typing of L. monocytogenes by RAPD technique the present study was undertaken to type isolates of L. monocytogenes from clinical, food and veterinary samples.
  
  
  METHODS
  Isolates of L. monocytogenes were subjected to RAPD using four decamer random primers R1, R2, R3 and R4. Amplified products were analysed by agarose gel electrophoresis.
  
  
  RESULTS
  Eight strains of L. monocytogenes on RAPD analysis generated 4 distinct profiles each with R1 and R4 primers and 3 different profiles with R2 and R3 primers. The isolates from fish, clinical and veterinary samples showed different profiles with respect to each other. Isolate from flat fish (serovar 4) showed a different profile from that of clams (serovar 1). Two isolates from placenta (serovar 1) showed similar profiles and all the isolates from veterinary samples generated similar profiles.
  
  
  CONCLUSIONS
  RAPD analysis in the present study allowed discrimination of isolates among the same serotype but from different sources. Since RAPD is a rapid technique and offers greater discrimination of strains, this method may be used for typing L. monocytogenes in India.
authors.affiliation: Department of Microbiology, Kasturba Medical College, Mangalore, India.
authors.forename: B
authors.initials: B
authors.lastname: Dhanashree
authors.forename: S K
authors.initials: SK
authors.lastname: Otta
authors.forename: I
authors.initials: I
authors.lastname: Karunasagar
authors.forename: I
authors.initials: I
authors.lastname: Karunasagar
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000382:microbiology; D005260:Female; D005412:Flatfishes / Q000382:microbiology; D005516:Food Microbiology; D006801:Humans; D007194:India; D008089:Listeria monocytogenes / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010920:Placenta / Q000382:microbiology; D011247:Pregnancy; D019105:Random Amplified Polymorphic DNA Technique