title: In-process control of midazolam synthesis by HPLC.
abstract: A high-performance liquid chromatographic assay coupled with UV detection (239 nm) has been developed for the determination of midazolam and its synthesis precursors. The separation of the analytes was performed on a Kromasil C8 column (15 cm x 4.6 mm i.d., 5 microm) at 30 degrees C. The mobile phase [ammonium chloride (pH 5.5, 1 g l(-1))-methanolacetonitrile (45:22:33, v/v/v)] was pumped at a flow-rate of 1.5 ml min(-1). This method is rapid (less than 11 min), sensitive (limit of detection (LOD) ranged between 0.05 and 0.5 mg l(-1)) and selective for the determination of midazolam, and it could be used for monitoring different synthetic routes.
authors.affiliation: Asturpharma S.A., c/ Peña Brava, 22B-23, Silvota, 33192 Llanera, Asturias, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Laviana
authors.forename: I
authors.initials: I
authors.lastname: Llorente
authors.forename: M
authors.initials: M
authors.lastname: Bayod
authors.forename: D
authors.initials: D
authors.lastname: Blanco
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006993:Hypnotics and Sedatives / Q000138:chemical synthesis / Q000737:chemistry*; D017895:Manganese Compounds / Q000737:chemistry; D008874:Midazolam / Q000138:chemical synthesis / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
chemical_list: D006993:Hypnotics and Sedatives; D017895:Manganese Compounds; D010087:Oxides; D008874:Midazolam; C016552:manganese dioxide