[The effect of thyroliberin on the change in structure of various of lipid bilayer regions in biological membranes in vivo].
Thyroliberin (TRH) influence on microviscosity and thermoinduced structural transitions of biological membranes has been studied using spin probes and ESR technique. It was shown that TRH in three investigated concentrations (10(-6), 10(-10) and 10(-16) mol/l) in vivo resulted in increasing of the lipid microviscosity in the hydrophobic areas (20 A): the time of rotary correlation of 16-doxyl-stearic acid elevated by 17-50%. There were no statistically significant effects in the regions localized more close to the surface (8 A): the order parameter of 5-doxyl-stearic acid was not changed. The picture of thermoinduced structural transitions in described in this article. Under the action of TRH in vivo both the shift of structural transitions and the changes in their number have been observed. The results obtained indicated that the mechanism of the TRH effect has a non-receptor component.
Torchinskiĭ
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AA
Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, 119991 Russia. artremtor@yandex.ru
Pal'mina
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D000818:Animals; D002462:Cell Membrane / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004578:Electron Spin Resonance Spectroscopy; D004721:Endoplasmic Reticulum / Q000187:drug effects; D005227:Fatty Acids / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry*; D008099:Liver / Q000166:cytology / Q000187:drug effects; D008563:Membrane Lipids / Q000737:chemistry*; D051379:Mice; D008815:Mice, Inbred Strains; D013113:Spin Labels; D013696:Temperature; D013973:Thyrotropin-Releasing Hormone / Q000494:pharmacology*; D014783:Viscosity
D005227:Fatty Acids; D008051:Lipid Bilayers; D008563:Membrane Lipids; D013113:Spin Labels; D013973:Thyrotropin-Releasing Hormone