title: Neuropeptide Y is neuroproliferative for post-natal hippocampal precursor cells.
abstract: New neurones are produced in the adult hippocampus throughout life and are necessary for certain types of hippocampal learning. Little, however, is known about the control of hippocampal neurogenesis. We used primary hippocampal cultures from early post-natal rats and neuropeptide Y Y1 receptor knockout mice as well as selective neuropeptide Y receptor antagonists and agonists to demonstrate that neuropeptide Y is proliferative for nestin-positive, sphere-forming hippocampal precursor cells and beta-tubulin-positive neuroblasts and that the neuroproliferative effect of neuropeptide Y is mediated via its Y1 receptor. Immunohistochemistry confirmed Y1 receptor staining on both nestin-positive cells and beta-tubulin-positive cells in culture and short pulse 5-bromo-2-deoxyuridine studies demonstrated that neuropeptide Y has a proliferative effect on both cell types. These studies suggest that the proliferation of hippocampal neuroblasts and precursor cells is increased by neuropeptide Y and, therefore, that hippocampal learning and memory may be modulated by neuropeptide Y-releasing interneurones.
authors.affiliation: Division of Clinical Neurosciences, University of Southampton, Southampton, UK CNRRR, Helen Hayes Hospital, West Haverstraw, New York, USA.
authors.forename: Owain W
authors.initials: OW
authors.lastname: Howell
authors.forename: Helen E
authors.initials: HE
authors.lastname: Scharfman
authors.forename: Herbert
authors.initials: H
authors.lastname: Herzog
authors.forename: Lars E
authors.initials: LE
authors.lastname: Sundstrom
authors.forename: Annette
authors.initials: A
authors.lastname: Beck-Sickinger
authors.forename: William P
authors.initials: WP
authors.lastname: Gray
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000943:Antigens, Differentiation / Q000096:biosynthesis; D001973:Bromodeoxyuridine; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D007381:Intermediate Filament Proteins / Q000096:biosynthesis; D051379:Mice; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins; D064231:Nestin; D009478:Neuropeptide Y / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D017476:Receptors, Neuropeptide Y / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D018874:Spheroids, Cellular / Q000166:cytology / Q000187:drug effects; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D014404:Tubulin / Q000096:biosynthesis
chemical_list: D000943:Antigens, Differentiation; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; C577486:Nes protein, rat; D064231:Nestin; D009478:Neuropeptide Y; D017476:Receptors, Neuropeptide Y; D014404:Tubulin; C097212:neuropeptide Y-Y1 receptor; D016222:Fibroblast Growth Factor 2; D001973:Bromodeoxyuridine