title: Effects of short-term racing activity on platelet and neutrophil activation in dogs.
abstract: OBJECTIVE
  To determine whether platelets and neutrophils become activated in dogs during short-distance sled-pulling activity.
  
  
  METHODS
  18 physically fit adult Siberian Huskies.
  
  
  METHODS
  Dogs were allocated into 2 teams (9 dogs/team). Each team ran a course of approximately 6.4 km while pulling a sled that contained 2 people. Blood samples were collected immediately before and within 10 minutes after completion of sled-pulling activity. Blood was aspirated into sterile syringes and immediately transferred to evacuated tubes containing EDTA solution. Platelet activation status was evaluated by determining cell-surface P-selection expression, number of platelet aggregates and platelet microparticles, mean platelet-component (MPC) concentration, and mean platelet-component distribution width (MPCDW) concentration. Neutrophil activation status was evaluated by determining cell-surface CD11/CD18 expression, neutrophil size, and neutrophil granularity.
  
  
  RESULTS
  Short-duration strenuous sled-pulling activity was associated with lower MPC concentration, higher MPCDW concentration, and higher cell-surface P-selectin expression after activation with phorbol myristate acetate. An increase in neutrophil CD11/CD18 expression and a decrease in neutrophil granularity were also observed after exercise.
  
  
  CONCLUSIONS
  Results of this study provide evidence of priming and activation of platelets and activation of neutrophils after strenuous short-duration sled-pulling activity. Additional studies will be needed to determine whether these changes have adverse effects on animal performance or induce tissue injury.
authors.affiliation: Clinic for Internal Medicine and Forensic Affairs, Faculty of Veterinary Medicine, Justus-Liebig-University, Giessen, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Moritz
authors.forename: Bruce K
authors.initials: BK
authors.lastname: Walcheck
authors.forename: Jennifer
authors.initials: J
authors.lastname: Deye
authors.forename: Douglas J
authors.initials: DJ
authors.lastname: Weiss
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000502:physiology; D004285:Dogs; D005260:Female; D007958:Leukocyte Count; D008297:Male; D018375:Neutrophil Activation; D009504:Neutrophils / Q000502:physiology; D010805:Physical Conditioning, Animal / Q000502:physiology*; D005082:Physical Exertion / Q000502:physiology*; D015539:Platelet Activation