Capillary electrophoretic separation and quantification of flavone-O- and C-glycosides in Achillea setacea W. et K.
A simple method for the rapid separation and quantification of flavone-O- and C-glycosides in A. setacea W. et K. by capillary zone electrophoresis (CZE) with UV detection is described. Using 25 mM sodium borate with 20% (v/v) of methanol (pH 9.3) as running buffer sufficient separation of the analytes was achieved within 19 min. For the quantitative determination isorhamnetin-3-O-rutinoside was used as internal standard. The method was successfully applied to a rapid characterisation of the flavonoid complex and a precise quantification of the single and total amount of the flavonoids in different samples of A. setacea.
Marchart
Elke
E
Institute of Pharmacognosy, PharmaCenterVienna, University of Vienna, Althanstrasse 14, 1090 Vienna, Austria.
Kopp
Brigitte
B
D031162:Achillea / Q000737:chemistry*; D002855:Chromatography, Thin Layer; D019075:Electrophoresis, Capillary / Q000379:methods*; D005419:Flavonoids / Q000737:chemistry*; D006027:Glycosides / Q000032:analysis*
D005419:Flavonoids; D006027:Glycosides