Phospholipids from Bombycis corpus and their neurotrophic effects.
Three phospholipids (4-6) and three aromatic amines (1-3) were obtained from the methanol extract of Bombycis corpus. Based on spectral data, their structures have been elucidated as nicotiamide (1), cytidine (2), adenine (3), 1-O-(9Z-octadecenoyl)-2-O-(8Z,11Z-octadecadienoyl) sn-glycero-3-phosphorylcholine (4), 1,2-di-O-hexadecanoyl-sn-glycero-3-phosphorylcholine (5) and 1,2-di-O-9Z-octadecenoyl-sn-glycero-3-phosphorylcholine (6). We examined the effects of compounds on synthesis of NGF in cultured astrocytes. By RT-PCR analysis, expresison of NGF mRNA in astrocytes cultured in serum-starvation increased after the addition of phospholipid (10 microM). The NGF content in the culture medium was significantly increased by compound 5, compared with the control value. These results suggest that three phospholipid compounds isolated from the methanol extract of Bombycis corpus may exert neurotrophic effects by stimulation of NGF synthesis in astrocytes.
Kwon
Hak Cheol
HC
College of Pharmacy, SungKyunKwan University, Suwon 440-746, Korea.
Jung
I Yeon
IY
Cho
Se Yeon
SY
Cho
Ock Ryun
OR
Yang
Min Cheol
MC
Lee
Sung Ok
SO
Hur
Jin Young
JY
Kim
Sun Yeou
SY
Yang
Jong Beom
JB
Lee
Kang Ro
KR
D000818:Animals; D009414:Nerve Growth Factors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D016716:PC12 Cells; D010743:Phospholipids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D028321:Plant Preparations / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats
D009414:Nerve Growth Factors; D010743:Phospholipids; D010936:Plant Extracts; D028321:Plant Preparations