Determination of free salicylic acid in chewing aspirin tablets by HPLC.
OBJECTIVE
To establish a HPLC method for determining the content of free salicylic acid in chewing aspirin tablets.


METHODS
The determination was conducted on a HPLC column (C(18), 150 mm x 4.6 mm x 5 microm) with methanol-water-glacial acetic acid (8.0 5.5 1.0) as the mobile phase and the detection wavelength of 302 nm.


RESULTS
The calibration curve was linear within the concentration range of 2.65 to 31.77 microg/ml (r=0.999 97) of salicylic acid. The average recovery rate was 100.21% with relative standard deviation of 0.53% (n=6).


CONCLUSIONS
HPLC is quick and accurate of determining the content of free salicylic acid for chewing aspirin tablets.
Tian
Jun
J
Department of Pharmacy, General Navy Hospital of PLA, Beijing 100037, China.
Chen
Xin-shan
XS
Wang
Rui-dong
RD
D001241:Aspirin / Q000008:administration & dosage / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D008409:Mastication; D020156:Salicylic Acid / Q000032:analysis*; D013607:Tablets
D013607:Tablets; D020156:Salicylic Acid; D001241:Aspirin