title: Evidence of the presence of Seoul virus in Cambodia.
abstract: A study was conducted in agricultural and urban areas in Cambodia to assess the presence of hantaviruses in rodent populations. In 1998, rodents were trapped in two villages and in Phnom Penh city around market places and a rubbish dump. IgG antibodies to Hantaan virus were detected in 54 (8.2%) rodents among 660 tested: 6.4% (13/203) among roof rats (Rattus rattus), 20.9% (39/187) among Norway rats (R. norvegicus), 16.7% (2/12) among unidentified Rattus species and none in 183 Polynesian rats (R. exulans) or in 75 bandicoot rats (Bandicota sp.). The presence of the viral genome was detected by a reverse transcription-PCR amplifying part of the sequence coding for the nucleoprotein in the S segment, in 87% of the seropositive rodents. Thirty-one representative cDNAs were sequenced. Phylogenetic studies of the sequences indicated a close relationship with Seoul virus. However, the Cambodian Seoul virus sequences clustered within two different phylogenetic lineages, one associated with R. rattus and the other with R. norvegicus.
authors.affiliation: Institut Pasteur du Cambodge, 5 boulevard Monivong, BP 983, Phnom Penh, Royaume du Cambodge, Cambodia. jmreynes@pasteur-kh.org
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Reynes
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Soares
authors.forename: Thomas
authors.initials: T
authors.lastname: Hüe
authors.forename: Michèle
authors.initials: M
authors.lastname: Bouloy
authors.forename: Sot
authors.initials: S
authors.lastname: Sun
authors.forename: Sun Lay
authors.initials: SL
authors.lastname: Kruy
authors.forename: François
authors.initials: F
authors.lastname: Flye Sainte Marie
authors.forename: Hervé
authors.initials: H
authors.lastname: Zeller
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002160:Cambodia; D018076:DNA, Complementary; D018778:Hantavirus Infections / Q000453:epidemiology / Q000662:veterinary*; D007074:Immunoglobulin G / Q000097:blood; D009115:Muridae / Q000276:immunology / Q000821:virology*; D010802:Phylogeny; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012376:Rodent Diseases / Q000453:epidemiology*; D029263:Seoul virus / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D000914:Antibodies, Viral; D018076:DNA, Complementary; D007074:Immunoglobulin G