title: Comparison of Plasmodium falciparum transfection methods.
abstract: The development of an electroporation based transfection method for Plasmodium falciparum has been very successful for the study of some genes but its efficiency remains very low. While alternative approaches have been documented, electroporation of infected red blood cells generally remains the method of choice for introducing DNA into P. falciparum. In this paper we compare four published transfection techniques in their ability to achieve stable transfections.
authors.affiliation: Malaria Biology Laboratory, The Australian Centre for International and Tropical Health and Nutrition, University of Queensland, Australia. tinaS@qimr.edu.au
authors.forename: Tina S
authors.initials: TS
authors.lastname: Skinner-Adams
authors.forename: Paula M
authors.initials: PM
authors.lastname: Lawrie
authors.forename: Paula L
authors.initials: PL
authors.lastname: Hawthorne
authors.forename: Donald L
authors.initials: DL
authors.lastname: Gardiner
authors.forename: Katharine R
authors.initials: KR
authors.lastname: Trenholme
mesh_terms: D000818:Animals; D018274:Electroporation / Q000379:methods; D004912:Erythrocytes / Q000737:chemistry / Q000378:metabolism / Q000469:parasitology; D005822:Genetic Vectors / Q000235:genetics; D010963:Plasmodium falciparum / Q000235:genetics*; D014162:Transfection / Q000379:methods*