title: [Generation of recombinant human tissue factor pathway inhibitor in E. coli].
abstract: Tissue factor pathway inhibitor (TFPI) is one of the major physiological inhibitors of the human blood coagulation cascade and may have great potential in the prevention and therapy of diseases caused by thrombus formation. In this study, recombinant human tissue factor was generated in E. coli containing a recombinant vector constructed by inserting TFPI cDNA into pGEX-2T vector. The generated recombinant TFPI (rTFPI) could be simply purified with glutathione-agarose affinity method and maintained its biological function in terms of inhibition of tissue factor and factor Xa.
authors.affiliation: Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Bioactive Materials Key Lab of Ministry of Education, Nankai University, Tianjin.
authors.forename: Xigang
authors.initials: X
authors.lastname: Leng
authors.forename: Chuanhua
authors.initials: C
authors.lastname: Wang
authors.forename: Liping
authors.initials: L
authors.lastname: Song
authors.forename: Xiaozheng
authors.initials: X
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Ma
authors.forename: Pengyan
authors.initials: P
authors.lastname: Wang
mesh_terms: D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008074:Lipoproteins / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D010446:Peptide Fragments / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000494:pharmacology; D014162:Transfection
chemical_list: D008074:Lipoproteins; D010446:Peptide Fragments; D011994:Recombinant Proteins; C081249:tissue factor pathway inhibitor (1-161)