title: [Exclusive gene mapping on retinitis pigmentosa with markers on chromosomes 3 in a Chinese kindred].
abstract: OBJECTIVE
  To study the relationship between the rhodopsin gene on chromosome 3 and autosomal dominant retinitis pigmentosa (ADRP) in a Chinese kindred.
  
  
  METHODS
  Sixteen normal persons and 18 RP patients in a ADRP family were recruited. Genome scan method based on fluorescence labeled (using 3 different labels: 6-FAM, HEX, and NED) microsatellite markers with multiplex PCR system was used to identify loci influencing susceptibility to ADRP. Fourteen microsatellites (D3S1297, D3S1263, D3S1266, D3S1289, D3S1300, D3S3681, D3S1271, D3S1292, D3S1569, D3S1279, D3S1614, D3S1262, D3S1580 and D3S1311) on chromosome 3 were used as genetic markers. Linkage analysis (using Genescan3.0, GeneScan Analysis 2.1, Genotyper 2.1 and Designer sofe system) was performed using these markers.
  
  
  RESULTS
  The LOD value was <or= -2 in all 14 microsatellites in chromosome 3, indicating that there was no linkage between these markers and RP related gene in this kindred.
  
  
  CONCLUSIONS
  The RP related gene in this kindred is located on chromosomes other than chromosome 3 and is not caused by the mutation of rhodopsin gene.
authors.affiliation: National Laboratory of Medical Genetics of China, Central South University, Changsha, 410078, China. liuzheng19752002@yahoo.com.cn
authors.forename: Zheng
authors.initials: Z
authors.lastname: Liu
authors.forename: Hao
authors.initials: H
authors.lastname: Deng
authors.forename: Xing-hua
authors.initials: XH
authors.lastname: Xi
authors.forename: Jia-hui
authors.initials: JH
authors.lastname: Xia
authors.forename: Qian
authors.initials: Q
authors.lastname: Pan
authors.forename: He-ping
authors.initials: HP
authors.lastname: Dai
authors.forename: Yong-jia
authors.initials: YJ
authors.lastname: Yang
authors.forename: Han-xiang
authors.initials: HX
authors.lastname: Deng
authors.forename: Kun
authors.initials: K
authors.lastname: Xia
authors.forename: Duo
authors.initials: D
authors.lastname: Zheng
mesh_terms: D044466:Asian People; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics*; D006801:Humans; D018895:Microsatellite Repeats; D010375:Pedigree; D012174:Retinitis Pigmentosa / Q000235:genetics*; D012243:Rhodopsin / Q000235:genetics
chemical_list: D012243:Rhodopsin