title: Modified laser speckle imaging method with improved spatial resolution.
abstract: A two-dimensional map of blood flow is crucial for physiological studies. We present a modified laser speckle imaging method (LSI) that is based on the temporal statistics of a time-integrated speckle. A model experiment was performed for the validation of this technique. The spatial and temporal resolutions of this method were studied in theory and compared with current laser speckle contrast analysis (LASCA); the comparison indicates that the spatial resolution of the modified LSI is five times higher than that of current LASCA. Cerebral blood flow under different temperatures was investigated by our modified LSI. Compared with the results obtained by LASCA, the blood flow map obtained by the modified LSI possessed higher spatial resolution and provided additional information about changes in blood perfusion in small blood vessels. These results suggest that this is a suitable method for imaging the full field of blood flow without scanning and provides much higher spatial resolution than that of current LASCA and other laser Doppler perfusion imaging methods.
authors.affiliation: Huazhong University of Science and Technology, The Key Laboratory of Biomedical Photonics of the Ministry of Education, Department of Biomedical Engineering, Wuhan, Hubei, China 430074.
authors.forename: Haiying
authors.initials: H
authors.lastname: Cheng
authors.forename: Qingming
authors.initials: Q
authors.lastname: Luo
authors.forename: Shaoqun
authors.initials: S
authors.lastname: Zeng
authors.forename: Shangbin
authors.initials: S
authors.lastname: Chen
authors.forename: Jian
authors.initials: J
authors.lastname: Cen
authors.forename: Hui
authors.initials: H
authors.lastname: Gong
mesh_terms: D000465:Algorithms; D000818:Animals; D001783:Blood Flow Velocity / Q000502:physiology; D001921:Brain / Q000098:blood supply* / Q000166:cytology* / Q000502:physiology; D002560:Cerebrovascular Circulation / Q000502:physiology*; D003936:Diagnosis, Computer-Assisted / Q000379:methods*; D011786:Quality Control; D051381:Rats; D017207:Rats, Sprague-Dawley; D012212:Rheology / Q000295:instrumentation / Q000379:methods*; D013696:Temperature