Saccharomyces cerevisiae expression vectors with thrombin-cleavable N- and C-terminal 6x(His) tags.
General-purpose yeast expression vectors for convenient cloning and production of proteins with N- or C-terminal His6 tags that can be efficiently removed with thrombin have been developed. To the parental yeast-E. coli shuttle vectors that have convenient copper-inducible expression, two selectable markers and LEU2d vector amplification, this development adds substantial versatility to product recovery.
Patel
Onisha
O
CSIRO Health Sciences and Nutrition, 343 Royal Parade, Parkville, Victoria, Australia. onisha.patel@csiro.au
Fernley
Ross
R
Macreadie
Ian
I
D019976:Cloning, Organism / Q000379:methods; D004789:Enzyme Activation; D020224:Expressed Sequence Tags; D015966:Gene Expression Regulation, Fungal / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D010453:Peptide Synthases / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D013917:Thrombin / Q000235:genetics* / Q000378:metabolism
D011994:Recombinant Proteins; D013917:Thrombin; D010453:Peptide Synthases; C020041:dihydrofolate synthetase