C-C chemokine receptor 2 and sarcoidosis: association with Lofgren's syndrome.
Sarcoidosis is thought to result from the interaction between an unknown environmental antigenic trigger and the host's genetic susceptibility. We hypothesized that sarcoidosis, or one of the disease subsets, could be associated with single nucleotide polymorphisms of C-C chemokine receptor 2 (CCR2) gene. Eight single-nucleotide polymorphisms in CCR2 were studied in a total of 304 Dutch individuals (90 non-Löfgren sarcoidosis, 47 Löfgren's syndrome, 167 control subjects). From the investigated CCR2 polymorphisms, nine haplotypes were deduced (haplotypes 1-9). In patients with Löfgren's syndrome, a strongly significant increase in the frequency of CCR2-haplotype 2, which includes four unique alleles (A at nucleotide position -6752, A at 3,000, T at 3,547, and T at 4,385), was observed compared with control subjects (74% vs. 38% respectively, p < 0.0001), whereas no difference was found between non-Löfgren sarcoidosis and control subjects (both 38%). The association between CCR2-haplotype 2 carriage frequency and Löfgren's syndrome (odds ratio, 4.4; p < 0.0001) remained significant after adjustment for human leukocyte antigen haplotype DRB1*0301-DQB1*0201 (odds ratio, 11.5; p < 0.0001) and female sex (odds ratio, 3.2; p = 0.003), two known risk factors for Löfgren's syndrome. In conclusion, this report describes a strong association between CCR2-haplotype 2 and Löfgren's syndrome. Further studies are needed to understand the molecular mechanisms underlying this association.
Spagnolo
Paolo
P
Clinical Genomics Group, Department of Occupational and Environmental Medicine, Imperial College of Science, Technology and Medicine, 1B Manresa Road, London SW3 6LR, UK.
Renzoni
Elisabetta A
EA
Wells
Athol U
AU
Sato
Hiroe
H
Grutters
Jan C
JC
Sestini
Piersante
P
Abdallah
Atiyeh
A
Gramiccioni
Enzo
E
Ruven
Henk J T
HJ
du Bois
Roland M
RM
Welsh
Kenneth I
KI
D018771:Arthralgia / Q000235:genetics*; D004893:Erythema Nodosum / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D006683:HLA-DQ Antigens / Q000235:genetics; D059866:HLA-DQ beta-Chains; D006684:HLA-DR Antigens / Q000235:genetics; D059811:HLA-DRB1 Chains; D006239:Haplotypes / Q000235:genetics; D006579:Heterozygote; D006801:Humans; D008206:Lymphatic Diseases / Q000235:genetics*; D008297:Male; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D054390:Receptors, CCR2; D019707:Receptors, Chemokine / Q000235:genetics*; D017565:Sarcoidosis, Pulmonary / Q000235:genetics*; D013577:Syndrome
C515334:CCR2 protein, human; D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; C553502:HLA-DRB1*03:01 antigen; D054390:Receptors, CCR2; D019707:Receptors, Chemokine