title: Germ cell transplantation: a review and progress report on ICSI from spermatozoa generated in xenogeneic testes.
abstract: Results from the transplantation of donor male germ cells into xenogeneic recipient seminiferous tubules indicate that donor spermatogonia are capable of differentiating to form spermatozoa morphologically characteristic of the donor species. Germ cell transplantation procedures combined with developments in freezing, culturing or enriching germ cell populations have applications of paramount importance in medicine, basic sciences and animal reproduction. Additionally, these techniques can serve as an alternative approach for gonadal protection and fertility preservation in patients with cancer. This article is a chronological critical review of the technological advances that followed the initial successful transplantation of mouse germ cells into recipient mice. Furthermore, the factors responsible for the immunological privilege properties of the testis and the parameters influencing the potential of mammalian germ cells to undergo mitosis and meiosis within a xenogeneic testis are described. Finally, the role of human germ cell transplantation procedures in the therapeutic management of non-obstructive azoospermia is discussed.
authors.affiliation: Department of Urology, Tottori University School of Medicine, Nishimachi, Yonago, Japan. akrosnin@hotmail.com
authors.forename: N
authors.initials: N
authors.lastname: Sofikitis
authors.forename: A
authors.initials: A
authors.lastname: Kaponis
authors.forename: Y
authors.initials: Y
authors.lastname: Mio
authors.forename: D
authors.initials: D
authors.lastname: Makredimas
authors.forename: D
authors.initials: D
authors.lastname: Giannakis
authors.forename: Y
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: N
authors.initials: N
authors.lastname: Kanakas
authors.forename: H
authors.initials: H
authors.lastname: Kawamura
authors.forename: J
authors.initials: J
authors.lastname: Georgiou
authors.forename: M
authors.initials: M
authors.lastname: Schrader
authors.forename: E
authors.initials: E
authors.lastname: Lolis
authors.forename: X
authors.initials: X
authors.lastname: Giannakopoulos
authors.forename: D
authors.initials: D
authors.lastname: Loutradis
authors.forename: V
authors.initials: V
authors.lastname: Tarlatzis
authors.forename: I
authors.initials: I
authors.lastname: Miyagawa
mesh_terms: D000818:Animals; D017690:Cell Transplantation / Q000379:methods; D015925:Cryopreservation; D006801:Humans; D007700:Kinetics; D008297:Male; D008540:Meiosis / Q000502:physiology; D012671:Seminiferous Tubules; D020554:Sperm Injections, Intracytoplasmic; D013091:Spermatogenesis / Q000502:physiology; D013094:Spermatozoa / Q000166:cytology / Q000637:transplantation*; D013737:Testis / Q000502:physiology*; D014019:Tissue Donors; D014183:Transplantation, Heterologous