Development of dimethyl sulfoxide biosensor using a mediator immobilized enzyme electrode.
A mediator immobilized dimethyl sulfoxide (DMSO) sensor using DMSO reductase (DMSO-R) was constructed. Methyl viologen (MV) was used as a mediator and immobilized on a glassy carbon (GC) electrode with Nafion polymer. DMSO-R from Rhodobacter sphaeroides f. sp. denitrificans was retained by a dialysis film on the modified GC electrode. The amperometric signal in response to DMSO was observed. The linear range of the calibration curve for DMSO was between 0 and 600 microM. The response time was within 100 s and the relative standard deviation was 4% at 200 microM DMSO (n = 4). To eliminate the background noise derived from oxygen in samples, the glucose oxidase-catalase retained DMSO sensor was also examined.
Cheng
Hsu-chih
HC
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo, Tokyo 113-8657, Japan.
Abo
Mitsuru
M
Okubo
Akira
A
D015374:Biosensing Techniques; D004121:Dimethyl Sulfoxide; D004566:Electrodes; D004798:Enzymes
D004798:Enzymes; D004121:Dimethyl Sulfoxide