[Role of lipid peroxidation in hepatic ischemia-reperfusion injury and effect of L-Arginine].
OBJECTIVE
To study the role of lipid peroxidation during hepatic ischemia-reperfusion injury (HIRI) and the effects of L-Arginine (L-Arg).


METHODS
The rabbits and patients who underwent elective hepatic surgery were randomly divided into two groups respectively, HIRI group and L-Arg group.Plasma malondialdehyde (MDA) and alanine aminotransferase (ALT) were measured before ischemia 45 minutes (rabbits) or 25 minutes(patients) and 45 minutes(rabbits) or 25 minutes (patients) after reperfusion.


RESULTS
As compared with pre-ischemia, MDA and ALT increased during ischemia 45 minutes (or 25 minutes) in rabbits and patients, P<0.05 or P<0.01. Treatment with L-Arg could markedly reduce the elevation of mentioned biochemical parameters during hepatic ischemia. In rabbits MDA decreased on hepatic but the different were not significant (P>0.05), ALT decreased (P<0.05). In patients MDA and ALT both decreased on hepatic(both P<0.01). After reperfusion, MDA and ALT all decreased in rabbits and patients (all P<0.01).


CONCLUSIONS
These data suggest that lipid peroxidation can play an important role in the happening and developing of HIRI. Treatment with L-Arg may attenuate ischemia-reperfusion injury by antagonizing lipid peroxidation.
Wang
Wan-tei
WT
Department of Pathophysiology, Wenzhou Medical College, Wenzhou 325027, Zhejiang, China.
Wang
Wei
W
Xu
Zheng-jie
ZJ
Lin
Li-na
LN
Li
Dong
D
D000328:Adult; D000368:Aged; D000818:Animals; D001120:Arginine / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation; D008107:Liver Diseases / Q000188:drug therapy / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D011184:Postoperative Period; D011817:Rabbits; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism*
D001120:Arginine