title: Transcriptional expression of Escherichia coli glutamate-dependent acid resistance genes gadA and gadBC in an hns rpoS mutant.
abstract: Resistance to being killed by acidic environments with pH values lower than 3 is an important feature of both pathogenic and nonpathogenic Escherichia coli. The most potent E. coli acid resistance system utilizes two isoforms of glutamate decarboxylase encoded by gadA and gadB and a putative glutamate:gamma-aminobutyric acid antiporter encoded by gadC. The gad system is controlled by two repressors (H-NS and CRP), one activator (GadX), one repressor-activator (GadW), and two sigma factors (sigma(S) and sigma(70)). In contrast to results of previous reports, we demonstrate that gad transcription can be detected in an hns rpoS mutant strain of E. coli K-12, indicating that gad promoters can be initiated by sigma(70) in the absence of H-NS.
authors.affiliation: Division of Human Immunology, Hanson Institute, Institute of Medical and Veterinary Science, Adelaide, South Australia, 5000, Australia. scott.waterman@imvs.sa.gov.au
authors.forename: Scott R
authors.initials: SR
authors.lastname: Waterman
authors.forename: P L C
authors.initials: PL
authors.lastname: Small
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005968:Glutamate Decarboxylase / Q000235:genetics / Q000378:metabolism*; D005971:Glutamates / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D012808:Sigma Factor / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C491226:GadC protein, E coli; C103589:GadC protein, bacteria; D005971:Glutamates; C031908:H-NS protein, bacteria; D008565:Membrane Proteins; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria; C121044:gadA protein, E coli; D005968:Glutamate Decarboxylase