Expression of LL-37/hCAP-18 gene in human leukemia cells.
LL-37/hCAP-18 is an important part of host defense. Several diseases in human are characterized by impairment in the function of LL-37/hCAP-18 peptide. We examined the expression of LL-37/hCAP-18 in a panel of hematopoietic cell lines representing multiple cell lineages. LL-37/hCAP-18 expression at mRNA level was detected and varied among six of nine cell lines. The level of Raji cells was about eight folds higher than that of Ramos cells. However, only two cell lines, J6-1 and U937, expressed protein products. We also investigated LL-37/hCAP-18 protein in nine leukemia and three idiopathic thrombocytopenic purpura (ITP) patients via immunocytochemical staining. The rate of LL-37/hCAP-18 positive cells ranged from 60 (ITP) to 0.5% (M5). These data suggested that the low translation efficiency of LL-37/hCAP-18 expresses in some leukemia cells might be one of the reasons that leukemia patients were susceptible to infection.
Yang
Ying-Hua
YH
National Laboratory of Experimental Hematology, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, 288 Nanjing Road, Tianjin 300020, PR China.
Zheng
Guo-Guang
GG
Li
Ge
G
Zhang
Bin
B
Song
Yu-Hua
YH
Wu
Ke-Fu
KF
D000328:Adult; D000368:Aged; D023181:Antimicrobial Cationic Peptides / Q000032:analysis / Q000235:genetics*; D001773:Blood Cells / Q000473:pathology; D054804:Cathelicidins; D019070:Cell Lineage; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007938:Leukemia / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D014176:Protein Biosynthesis; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis; D014407:Tumor Cells, Cultured
D023181:Antimicrobial Cationic Peptides; D054804:Cathelicidins; D012333:RNA, Messenger