The effect of lumbrokinase on P-selectin and E-selectin in cerebral ischemia model of rat.
OBJECTIVE
To find the effect of lumbrokinase (LK) on P-selectin and E-selectin in ischemic rats.


METHODS
Male healthy Spragur-Dawley rats weighing 180-220 g (n = 90) were divided into 4 groups: (1) normal control group (n = 5), (2) sham-operated group (n = 35), (3) ischemic group (n = 35), (4) LK group (n = 15). LK 10 mg/kg (2000 UK activity of LK) was given by intraperitoneal injection in the LK group 30 minutes before experiment. Same volume of normal saline was given in the sham-operated group and ischemic group. The ischemic model was made by modified Haruo Nagasawa's method. Immunohistochemistry was used to observe the P-selectin and E-selectin positive cells in the ischemic region.


RESULTS
P-selectin and E-selectin positive cells in ischemic regions were observed in the ischemic group, and the peak of expression was at 6 hours and 12 hours, respectively. The similar changes were not observed in normal control group. There were only a few positive cells in the sham-operated group. In LK group, the P-selectin and E-selectin positive cells were significantly less than those in the ischemic group (P < 0.05 at 3 hours after the onset, P < 0.01 at 6 hours and P < 0.01 at 12 hours, respectively).


CONCLUSIONS
LK might significantly decrease the immunoreactions of P-selectin and E-selectin in ischemic lesion.
Zhang
Xiaoshu
X
Department of Neurology, PLA261 Hospital, Beijing, 100094.
Zhang
Jiatang
J
Kuang
Peizi
P
Lang
Senyang
S
Wu
Weiping
W
Yuan
Yumin
Y
Liu
Jiexiao
J
Liu
Yu
Y
Kuang
Peigen
P
D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism*; D015536:Down-Regulation; D019040:E-Selectin / Q000378:metabolism*; D010450:Endopeptidases / Q000494:pharmacology*; D008297:Male; D019007:P-Selectin / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D019040:E-Selectin; D019007:P-Selectin; D010450:Endopeptidases; C083421:lumbrokinase