title: Replication of bluetongue virus and epizootic hemorrhagic disease virus in pulmonary artery endothelial cells obtained from cattle, sheep, and deer.
abstract: OBJECTIVE
  To compare replication of bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV) in pulmonary artery endothelial cells (ECs) obtained from juvenile cattle, sheep, white-tailed deer (WTD; Odocoileus virginianus), and black-tailed deer (BTD; O hemionus columbianus).
  
  
  METHODS
  Cultures of pulmonary artery ECs obtained from 3 cattle, 3 sheep, 3 WTD, and 1 BTD.
  
  
  METHODS
  Purified cultures of pulmonary artery ECs were established. Replication, incidence of infection, and cytopathic effects of prototype strains of BTV serotype 17 (BTV-17) and 2 serotypes of EHDV (EHDV-1), and (EHDV-2) were compared in replicate cultures of ECs from each of the 4 ruminant species by use of virus titration and flow cytometric analysis.
  
  
  RESULTS
  All 3 viruses replicated in ECs from the 4 ruminant species; however, BTV-17 replicated more rapidly than did either serotype of EHDV. Each virus replicated to a high titer in all ECs, although titers of EHDV-1 were significantly lower in sheep ECs than in ECs of other species. Furthermore, all viruses caused extensive cytopathic effects and a high incidence of cellular infection; however, incidence of cellular infection and cytopathic effects were significantly lower in EHDV-1-infected sheep ECs and EHDV-2-infected BTD ECs.
  
  
  CONCLUSIONS
  There were only minor differences in replication, incidence of infection, and cytopathic effects for BTV-17, EHDV-1, or EHDV-2 in ECs of cattle, sheep, BTD, and WTD. It is not likely that differences in expression of disease in BTV- and EHDV-infected ruminants are attributable only to species-specific differences in the susceptibility of ECs to infection with the 2 orbiviruses.
authors.affiliation: Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis 95616, USA.
authors.forename: Bridget E
authors.initials: BE
authors.lastname: McLaughlin
authors.forename: Christopher D
authors.initials: CD
authors.lastname: DeMaula
authors.forename: William C
authors.initials: WC
authors.lastname: Wilson
authors.forename: Walter M
authors.initials: WM
authors.lastname: Boyce
authors.forename: N James
authors.initials: NJ
authors.lastname: MacLachlan
mesh_terms: D000818:Animals; D001820:Bluetongue virus / Q000502:physiology*; D002417:Cattle / Q000821:virology*; D003670:Deer / Q000821:virology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000821:virology*; D018164:Hemorrhagic Disease Virus, Epizootic / Q000502:physiology*; D011651:Pulmonary Artery / Q000166:cytology; D034561:Sheep, Domestic / Q000821:virology*; D013045:Species Specificity; D014779:Virus Replication