Evaluation of a polymerase chain reaction amplification method for Mycobacterium tuberculosis complex on samples from different sources.
Using primers specific for the IS6110 region of Mycobacterium tuberculosis complex, successful amplification by the polymerase chain reaction was demonstrated in 81 of 84 archive specimens from patients who had been clinically diagnosed 2 months to 16 y previously as having tuberculosis. Depending on the time of storage of the specimens, extra DNA bands were found in addition to the IS6110 region band.
Cataloluk
Osman
O
Department of Medical Biology and Genetics, Gaziantep University, Gaziantep, Turkey. ocatal@gantep.edu.tr
Karsligil
Tekin
T
Bayazit
Nurhayat
N
D015373:Bacterial Typing Techniques; D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012494:Sampling Studies; D012680:Sensitivity and Specificity; D013048:Specimen Handling; D014421:Turkey
D004269:DNA, Bacterial