Synthetic artificial peptidases and nucleases using macromolecular catalytic systems.
Effective artificial enzymes have been designed by adopting macromolecular systems for catalyst-substrate complexes. Artificial active sites comprising two or more organic functional groups were built on macromolecular backbones, leading to several types of organic artificial proteases. The activity of metal centers for peptide or DNA hydrolysis was greatly enhanced by attachment to polystyrene, leading to artificial metallopeptidases with substrate selectivity as well as artificial metallonucleases with high catalytic activity for double stranded DNA. A small artificial protease selective for a macromolecular target protein was synthesized. Target-specific artificial proteases can be used as protein-cleaving catalytic drugs.
Suh
Junghun
J
School of Chemistry and Center for Molecular Catalysis, Seoul National University, Seoul 151-747, Korea.
D002384:Catalysis; D046911:Macromolecular Substances; D045726:Metalloproteases / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D009696:Nucleic Acids / Q000378:metabolism; D010447:Peptide Hydrolases / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry / Q000378:metabolism; D013379:Substrate Specificity
D046911:Macromolecular Substances; D009696:Nucleic Acids; D011108:Polymers; D045726:Metalloproteases; D010447:Peptide Hydrolases