title: Evaluation of human beta-enolase as a serum marker for exercise-induced muscle damage.
abstract: OBJECTIVE
  To evaluate whether the serum beta-enolase level is a useful indicator of exercise-induced muscle damage in athletes.
  
  
  METHODS
  Blood samples were taken from 49 adult amateur marathon runners before and immediately after a marathon race, and the serum levels of beta-enolase and creatine phosphokinase were measured.
  
  
  METHODS
  The Aoshima Taiheiyo Marathon 2000, Miyazaki, Japan, on a cloudy day in December with an ambient temperature of 18 degrees C.
  
  
  METHODS
  Forty-nine adult amateur marathon runners (42 men and 7 women) who regularly participated in runs.
  
  
  METHODS
  The intervention was a marathon run.
  
  
  METHODS
  Serum beta-enolase was measured using a sensitive sandwich enzyme-linked immunosorbent assay. Serum creatine phosphokinase was measured using a standard procedure.
  
  
  RESULTS
  The mean beta-enolase concentration was 9.45 +/- 3.11 ng/mL before the race. It increased to 22.11 +/- 8.80 ng/mL after the race, representing a proportional increase of 1.57 +/- 1.46. The serum concentration of beta-enolase after the race was significantly higher than that before the race (P < 0.0001). Moreover, the serum beta-enolase level increased as much as the creatine phosphokinase level after the race, and strongly correlated with creatine phosphokinase (r = 0.828, P < 0.0001). The proportional increase of beta-enolase also correlated with that of creatine phosphokinase (r = 0.441, P < 0.005).
  
  
  CONCLUSIONS
  Our data suggest that the absolute values of the serum beta-enolase are more appropriate to relate to muscle damage.
authors.affiliation: Department of Orthopaedic Surgery, Miyazaki Medical College, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan. chosa@post.miyazaki-med.ac.jp
authors.forename: Etsuo
authors.initials: E
authors.lastname: Chosa
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Sekimoto
authors.forename: Norio
authors.initials: N
authors.lastname: Sonoda
authors.forename: Keitaro
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Matsuda
authors.forename: Keiichi
authors.initials: K
authors.lastname: Takahama
authors.forename: Naoya
authors.initials: N
authors.lastname: Tajima
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D015331:Cohort Studies; D003402:Creatine Kinase / Q000097:blood* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D015444:Exercise; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000201:enzymology* / Q000293:injuries*; D009135:Muscular Diseases / Q000201:enzymology / Q000503:physiopathology; D010751:Phosphopyruvate Hydratase / Q000097:blood* / Q000378:metabolism; D011336:Probability; D011379:Prognosis; D012420:Running / Q000293:injuries; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index
chemical_list: D015415:Biomarkers; D003402:Creatine Kinase; D010751:Phosphopyruvate Hydratase