title: The culture and establishment of embryonic germ (EG) cell lines from Chinese mini swine.
abstract: As a part of a basic research project on Xeno-transplantion, we have been engaged in the derivation of embryonic stem cell lines from Chinese mini swine. Here, we reported for the first time the establishment of two porcine EG cell lines (BPEG1 and BPEG2) from primordial germ cells of genital ridges of a 28 and a 27 d embryos respectively. Their pluripotent nature has been identified by colony morphology, marker characterization as well as by in vitro and in vivo differentiation. These porcine EG cells are potentially useful for further basic studies.
authors.affiliation: Laboratory of Embryonic Stem Cell, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China. escg@sunm.shcnc.ac.cn
authors.forename: Hsiao Chien
authors.initials: HC
authors.lastname: Tsung
authors.forename: Zhong Wei
authors.initials: ZW
authors.lastname: Du
authors.forename: Rong
authors.initials: R
authors.lastname: Rui
authors.forename: Xiu Lan
authors.initials: XL
authors.lastname: Li
authors.forename: Lin Ping
authors.initials: LP
authors.lastname: Bao
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.forename: Shi Min
authors.initials: SM
authors.lastname: Bao
authors.forename: Zhen
authors.initials: Z
authors.lastname: Yao
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D000954:Antigens, Surface / Q000276:immunology; D015415:Biomarkers / Q000032:analysis; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000502:physiology; D004268:DNA-Binding Proteins / Q000276:immunology; D004622:Embryo, Mammalian / Q000737:chemistry / Q000166:cytology* / Q000648:ultrastructure; D005260:Female; D005854:Germ Cells / Q000737:chemistry / Q000166:cytology* / Q000648:ultrastructure; D007150:Immunohistochemistry; D007621:Karyotyping; D008297:Male; D008854:Microscopy, Electron; D050814:Octamer Transcription Factor-3; D013234:Stem Cells / Q000737:chemistry / Q000166:cytology / Q000648:ultrastructure; D013552:Swine; D013556:Swine, Miniature; D014157:Transcription Factors
chemical_list: D000954:Antigens, Surface; D015415:Biomarkers; D004268:DNA-Binding Proteins; D050814:Octamer Transcription Factor-3; D014157:Transcription Factors; D000469:Alkaline Phosphatase