Effect of Cuscuta chinensis glycoside on the neuronal differentiation of rat pheochromocytoma PC12 cells.
Exposure of rat pheochromocytoma PC12 cells to Cuscuta chinensis glycoside induced neuronal differentiation with resulting outgrowth of neurites and increase of acetylcholinesterase activity. A specific inhibitor of mitogen-activated protein kinase (MAPK) kinase, PD98059, prevented this effect of C. chinensis on PC12 cells. These results suggested that C. chinensis glycoside induced neuronal differentiation in PC12 cells linked to the mitogen-activated protein kinase signaling cascade.
Jian-Hui
Liu
L
Department of Biochemical Engineering, Dalian University of Technology, Dalian 116024, PR China.
Bo
Jiang
J
Yong-Ming
Bao
B
Li-Jia
An
A
D000110:Acetylcholinesterase / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D034182:Cuscuta; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005419:Flavonoids / Q000494:pharmacology; D006027:Glycosides / Q000378:metabolism* / Q000494:pharmacology; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism*; D016501:Neurites / Q000187:drug effects; D009474:Neurons / Q000187:drug effects* / Q000201:enzymology; D016716:PC12 Cells; D028321:Plant Preparations / Q000494:pharmacology; D051381:Rats
D004365:Drugs, Chinese Herbal; D004791:Enzyme Inhibitors; D005419:Flavonoids; D006027:Glycosides; D028321:Plant Preparations; D020929:Mitogen-Activated Protein Kinase Kinases; D000110:Acetylcholinesterase; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one