title: Bone augmentation using rhGDF-5-collagen composite.
abstract: The aim of this study was to evaluate the effectiveness of local application of growth differentiation factor-5 (GDF-5)-collagen composite on bone augmentation on the rat calvaria. GDF-5-collagen composite is made from recombinant human GDF-5 (rhGDF-5) and purified bovine type I atelocollagen. The GDF-5 solution was mixed with 0.3% atelocollagen acid solution, and the mixture was lyophilized. The spongy lyophilized material was pressed into the shape of a minidisk to make the GDF-5-collagen composite. The GDF-5-collagen composite contained 1, 10, or 100-microg rhGDF-5. The control collagen composite contained 0-microg rhGDF-5. The GDF-5-collagen composite or control collagen composite was inserted beneath the calvarial periosteum of 4-week-old rats. At 3 weeks after implantation, the implants containing 1-microg rhGDF-5 had mostly induced new bone formation on the cranial side. In the implants containing 10- microg rhGDF-5, bone formation had proceeded to the center of the GDF-5-collagen composite from the periosteal and the cranial sides, and bone marrow was seen focally. The augmented bone showed a connected trabecular structure with abundant vascularization. The implants containing 100-microg rhGDF-5 were nearly entirely replaced by new bone with bone marrow, and the augmented bone was firmly connected with the original bone. Neither cartilage nor bone formation was found in the control collagen composite. Thus, we conclude that the GDF-5-collagen composite may be a superior biomaterial for bone augmentation and this composite could be useful as a local osteoinductive device.
authors.affiliation: Department of Fixed Prosthodontics, School of Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido, Japan.
authors.forename: Hiroya
authors.initials: H
authors.lastname: Kuniyasu
authors.forename: Yukito
authors.initials: Y
authors.lastname: Hirose
authors.forename: Morio
authors.initials: M
authors.lastname: Ochi
authors.forename: Akihiro
authors.initials: A
authors.lastname: Yajima
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Sakaguchi
authors.forename: Masaru
authors.initials: M
authors.lastname: Murata
authors.forename: Jens
authors.initials: J
authors.lastname: Pohl
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use; D001853:Bone Marrow / Q000187:drug effects / Q000473:pathology; D019485:Bone Morphogenetic Proteins / Q000008:administration & dosage / Q000627:therapeutic use*; D002417:Cattle; D003094:Collagen / Q000737:chemistry; D004337:Drug Carriers; D055428:Growth Differentiation Factor 5; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D010012:Osteogenesis / Q000187:drug effects*; D010521:Periosteum / Q000601:surgery; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins; D012886:Skull / Q000187:drug effects* / Q000473:pathology / Q000601:surgery; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000008:administration & dosage / Q000627:therapeutic use*
chemical_list: D001672:Biocompatible Materials; D019485:Bone Morphogenetic Proteins; D004337:Drug Carriers; C525893:GDF5 protein, human; D055428:Growth Differentiation Factor 5; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C059821:atelocollagen; D003094:Collagen