Oxime bond formation for the covalent attachment of oligonucleotides on glass support.
The chemical attachment of oligonucleotides on glass slides has been achieved using oxime bond formation. This method has been shown very efficient by comparison with the attachment of amino-oligonucleotides via reductive amination.
Defrancq
Eric
E
LEDSS, UMR CNRS 5616, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France. eric.defrancq@ujf-grenoble.fr
Hoang
Antoine
A
Vinet
Françoise
F
Dumy
Pascal
P
D000586:Amination; D005898:Glass / Q000737:chemistry*; D007091:Image Processing, Computer-Assisted; D017404:In Situ Hybridization, Fluorescence; D008956:Models, Chemical; D009841:Oligonucleotides / Q000138:chemical synthesis*; D010084:Oxidation-Reduction; D010091:Oximes / Q000138:chemical synthesis*; D012821:Silanes / Q000737:chemistry
D009841:Oligonucleotides; D010091:Oximes; D012821:Silanes