title: Flow and image cytometric DNA ploidy, including 5c exceeding cells, of serous borderline malignant ovarian tumors. Correlation with clinicopathologic characteristics.
abstract: OBJECTIVE
  To analyze DNA ploidy of serous borderline ovarian tumors by flow cytometry (FCM) and image cytometry (ICM), with 5c exceeding cells also analyzed, and to evaluate their correlation with clinicopathologic characteristics of patients and tumors.
  
  
  METHODS
  Cell suspensions were prepared according to a modified Hedley method from formalin-fixed, paraffin-embedded tissue blocks of 43 tumors. One part of the suspension was used for flow cytometric measurement; from the other part, filter slides were prepared for ICM.
  
  
  RESULTS
  FCM and ICM found 2 aneuploid (peridiploid) serous borderline ovarian tumors, and FCM found 1. ICM found 3 tumors with 5c exceeding cells and 2 tumors with octaploid cells. There was no correlation between DNA aneuploidy and presence of 5c exceeding cells with tumor size, International Federation of Gynecology and Obstetrics stage or survival.
  
  
  CONCLUSIONS
  The results confirm a good correlation between FCM and ICM DNA ploidy and the ability of ICM to detect 5c exceeding cells. The prognostic value of DNA ploidy and 5c exceeding cells in serous borderline malignant ovarian tumors warrant further evaluation.
authors.affiliation: Department of Cytopathology, Institute of Oncology, Zaloska 2, 1105 Ljubljana, Slovenia. mflezar@onko-i.si
authors.forename: Margareta Strojan
authors.initials: MS
authors.lastname: Flezar
authors.forename: Igor
authors.initials: I
authors.lastname: But
authors.forename: Rajko
authors.initials: R
authors.lastname: Kavalar
authors.forename: Marija
authors.initials: M
authors.lastname: Us-Krasovec
mesh_terms: D000782:Aneuploidy; D002467:Cell Nucleus / Q000473:pathology; D018284:Cystadenocarcinoma, Serous / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics*; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D005819:Genetic Markers; D006801:Humans; D019044:Image Cytometry / Q000379:methods*; D009364:Neoplasm Recurrence, Local; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D015203:Reproducibility of Results; D015996:Survival Rate
chemical_list: D004273:DNA, Neoplasm; D005819:Genetic Markers