title: Monitoring bacterial pathogens in the environment: advantages of a multilayered approach.
abstract: The application of advanced and highly sensitive molecular techniques to the detection of specific bacteria in the freshwater environment is limited, in the first instance, by sampling strategy and sample quality. Further combinations of molecular methods and techniques from apparently unrelated disciplines will ultimately shape the monitoring techniques of the future.
authors.affiliation: CEH Windermere, The Ferry House, Far Sawrey, Ambleside, Cumbria LA22 0LP, UK. roger@ceh.ac.uk
authors.forename: Roger W
authors.initials: RW
authors.lastname: Pickup
authors.forename: Glenn
authors.initials: G
authors.lastname: Rhodes
authors.forename: John
authors.initials: J
authors.lastname: Hermon-Taylor
mesh_terms: D001419:Bacteria / Q000302:isolation & purification*; D015374:Biosensing Techniques / Q000379:methods; D004783:Environmental Microbiology; D005618:Fresh Water / Q000382:microbiology*; D007118:Immunoassay / Q000379:methods*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D009862:Online Systems; D016133:Polymerase Chain Reaction / Q000379:methods*; D014871:Water Microbiology; D014873:Water Pollutants / Q000032:analysis*
chemical_list: D014873:Water Pollutants