Impact of transgenic technologies on functional genomics.
Gene transfer technologies in mammals are the focus of renewed interest owing to the recent emphasis on analyzing gene function in the postgenomic era. Three important developments in this area include transgenics, gene targeting and nuclear transfer or animal cloning. These technological innovations have enhanced our ability to analyze gene function at the level of the whole organism and have provided the means to modify gene expression. This review discusses the origins and current status of transgenic technologies. Various applications and technologies including chromosome engineering, stem cells, gene traps and modification of livestock are presented. The impact of mouse technologies and genomics on functional analyses is also discussed.
Shashikant
Cooduvalli S
CS
Dept. Dairy and Animal Science, College of Agricultural Sciences, 324 Henning, University Park, PA 16802, USA. css13@psu.edu
Ruddle
Frank H
FH
D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D001709:Biotechnology; D003001:Cloning, Molecular; D019976:Cloning, Organism; D019295:Computational Biology; D004195:Disease Models, Animal; D015870:Gene Expression; D018390:Gene Targeting; D005809:Genes, Regulator; D005816:Genetic Complementation Test; D015316:Genetic Therapy; D023281:Genomics; D006801:Humans; D051379:Mice; D013234:Stem Cells / Q000378:metabolism