title: Increased T-cell activation in decidua parietalis compared to decidua basalis in uncomplicated human term pregnancy.
abstract: OBJECTIVE
  The aim of this study was to quantify and compare activated T cells in term decidua basalis and parietalis using flow cytometry.
  
  
  METHODS
  Term decidua basalis and parietalis samples were obtained from 20 placentas collected after elective caesarean section. Percentages of leukocyte subclasses within the CD45+ cell fraction and activated T cells were determined by flow cytometry.
  
  
  RESULTS
  There was no significant difference in CD45+, CD14+, CD19+, and CD3+ cell percentages. However, within the CD3+ population, there were significantly more T-cell receptor-gamma(delta)+ (TCR-gamma(delta)-) and CD8+ cells in decidua parietalis compared with decidua basalis. More importantly, percentages of T cells expressing CD25, human leukocyte antigen (HLA)-DR, CD45RO, and CD69 markers were significantly increased in decidua parietalis.
  
  
  CONCLUSIONS
  These findings suggest that there are more activated T cells in decidua parietalis than in decidua basalis. Further investigation into differences between the two decidual sites may expand our understanding of the immunology of the maternal-fetal interface.
authors.affiliation: Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: A
authors.initials: A
authors.lastname: Sindram-Trujillo
authors.forename: S
authors.initials: S
authors.lastname: Scherjon
authors.forename: H
authors.initials: H
authors.lastname: Kanhai
authors.forename: D
authors.initials: D
authors.lastname: Roelen
authors.forename: F
authors.initials: F
authors.lastname: Claas
mesh_terms: D017252:CD3 Complex / Q000378:metabolism; D003656:Decidua / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D011247:Pregnancy; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
chemical_list: D017252:CD3 Complex