Site-directed mutagenesis using a single mutagenic oligonucleotide and DpnI digestion of template DNA.
Shenoy
Avinash R
AR
Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore, India.
Visweswariah
Sandhya S
SS
D002478:Cells, Cultured; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D004926:Escherichia coli / Q000166:cytology; D016297:Mutagenesis, Site-Directed; D009841:Oligonucleotides / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D013698:Templates, Genetic
D017931:DNA Primers; D009841:Oligonucleotides; D004247:DNA; C020102:endodeoxyribonuclease DpnI; D015252:Deoxyribonucleases, Type II Site-Specific