[Experimental study on cardiac protection and anti-oxidation effects of jisheng injection].
OBJECTIVE
To observe the effect of Jisheng injection (JSI) in protecting heart.


METHODS
Isolated heart of rat was preserved in modified Euro-Collins solution (mEC) containing JSI for 20 hrs, and that preserved in simple mEC was taken as control. Then Langendorff isolated rat heart perfusion was conducted. Forty minutes after perfusion, the cardiac function, coronary flow, myocardial water content were determined, and lactate dehydrogenase (LDH), creatine kinase (CK) activity in perfusate, superoxide dismutase (SOD) activity, malondialdehyde (MDA) content in myocardial tissue and pathologic change in myocardium were also observed.


RESULTS
The cardiac function and coronary flow of isolated heart preserved in JSI containing mEC was significantly better than those in the control (P < 0.01), with the LDH, CK activity and MDA content significantly lower (P < 0.01 and P < 0.05), SOD activity significantly higher (P < 0.05) and pathologic injury milder than those in control, but comparison of cardiac water content between the two groups showed insignificant difference.


CONCLUSIONS
JSI has good cardiac protective and anti-oxidizing effects.
Zhang
Su-qing
SQ
Department of Physiology, Beijing University of TCM, Beijing 100029. zhangsqing@sina.com.cn
Shi
Xue-jun
XJ
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006321:Heart; D008297:Male; D009926:Organ Preservation; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D020940:Reishi / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D019188:Organ Preservation Solutions; D013482:Superoxide Dismutase