title: Effects of circulating progesterone and insulin on early embryo development in beef heifers.
abstract: The aims of this study were to determine the effect on early embryo development of feeding a diet formulated to enhance circulating insulin concentrations and secondly to investigate the association between early embryo development and maternal progesterone concentrations in beef heifers. The study was carried out in 32 Simmental x Holstein Friesian heifers 22-25 months of age weighing 506+/-7kg and in condition score 3.1+/-0.1. Animals were fed two diets that were isoenergetic and isonitrogenous, but that would encourage either propionate (diet A) or acetate (diet B) production in the rumen. The rationale was that propionate would induce a greater insulin release in response to feeding. Animals were fed a 50:50 mix of the two diets for 14 days at 0.8x maintenance, with straw provided ad libitum. Animals were then fed one of the experimental diets for 3 weeks prior to synchronisation of oestrus and insemination and for a further 16 days following mating. All heifers were blood sampled daily from oestrus synchronisation and eight animals on each diet underwent daily transrectal real-time ultrasonography to determine the day of ovulation. All heifers were slaughtered at Day 16 after mating. While feeding of diet A (propionic) caused a significant (P<0.05) increase in the plasma insulin to glucagons ratio differences in insulin were not significantly different. This is probably due to the fact that insulin concentrations were quite high as the heifers used in the present study were in good body condition making further increases in insulin difficult to achieve. Diet did not affect size of ovulatory follicle (DIET A: 15.1+/-0.7mm; diet B: 14.6+/-0.7mm), day of ovulation (diet A: 3.5+/-0.2 days; diet B: 3.4+/-0.2 days), mean plasma progesterone concentration (diet A: 4.7+/-0.4ng/ml; diet B: 5.2+/-0.3ng/ml), corpus luteum weight (diet A: 6.0+/-0.2g; diet B: 6.0+/-0.2g) or pregnancy rate (diet A: 81.3%; diet B: 81.3%). However, the proportion of well-elongated (>10cm) embryos on Day 16 was higher in animals fed diet A than in those fed diet B (84.6% versus 38.5%; P<0.05). While progesterone concentration did not differ between pregnant and non-pregnant heifers, progesterone did show an earlier post-ovulatory rise in heifers with well-elongated (>10cm) embryos with levels in these animals significantly higher on Days 4 and 5 than in heifers with small (<10cm) embryos at slaughter. This study demonstrated an enhancement in early embryo development in animals fed a diet generating an increased insulin:glucagon ratio that was not related to circulating maternal progesterone concentrations. However, across diets, enhanced embryo development was associated with elevated plasma progesterone on Days 4 and 5 following mating.
authors.affiliation: School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Loughborough, LE12 5RD, UK. george.mann@nottingham.ac.uk
authors.forename: G E
authors.initials: GE
authors.lastname: Mann
authors.forename: M P
authors.initials: MP
authors.lastname: Green
authors.forename: K D
authors.initials: KD
authors.lastname: Sinclair
authors.forename: K J
authors.initials: KJ
authors.lastname: Demmers
authors.forename: M D
authors.initials: MD
authors.lastname: Fray
authors.forename: C G
authors.initials: CG
authors.lastname: Gutierrez
authors.forename: P C
authors.initials: PC
authors.lastname: Garnsworthy
authors.forename: R
authors.initials: R
authors.lastname: Webb
mesh_terms: D019342:Acetic Acid / Q000378:metabolism; D000818:Animals; D002417:Cattle / Q000196:embryology*; D003338:Corpus Luteum / Q000033:anatomy & histology; D004032:Diet; D005314:Embryonic and Fetal Development; D002149:Energy Intake; D005260:Female; D005934:Glucagon / Q000097:blood; D007328:Insulin / Q000097:blood*; D007700:Kinetics; D009929:Organ Size; D006080:Ovarian Follicle / Q000033:anatomy & histology; D010060:Ovulation; D011247:Pregnancy; D011374:Progesterone / Q000097:blood*; D011422:Propionates / Q000378:metabolism; D012417:Rumen / Q000378:metabolism
chemical_list: D007328:Insulin; D011422:Propionates; D011374:Progesterone; D005934:Glucagon; C029658:propionic acid; D019342:Acetic Acid