Surface salt bridges modulate DNA wrapping by the type II DNA-binding protein TF1.
The histone-like protein HU is involved in compaction of the bacterial genome. Up to 37 bp of DNA may be wrapped about some HU homologues in a process that has been proposed to depend on a linked disruption of surface salt bridges that liberates cationic side chains for interaction with the DNA. Despite significant sequence conservation between HU homologues, binding sites from 9 to 37 bp have been reported. TF1, an HU homologue that is encoded by Bacillus subtilis bacteriophage SPO1, has nM affinity for 37 bp preferred sites in DNA with 5-hydroxymethyluracil (hmU) in place of thymine. On the basis of electrophoretic mobility shift assays, we show that TF1-DNA complex formation is associated with a net release of only approximately 0.5 cations. The structure of TF1 suggests that Asp13 can form a dehydrated surface salt bridge with Lys23; substitution of Asp13 with Ala increases the net release of cations to approximately 1. These data are consistent with complex formation linked to disruption of surface salt bridges. Substitution of Glu90 with Ala, which would expose Lys87 predicted to contact DNA immediately distal to a proline-mediated DNA kink, causes an increase in affinity and an abrogation of the preference for hmU-containing DNA. We propose that hmU preference is due to finely tuned interactions at the sites of kinking that expose a differential flexibility of hmU- and T-containing DNA. Our data further suggest that the difference in binding site size for HU homologues is based on a differential ability to stabilize the DNA kinks.
Grove
Anne
A
Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana 70803, USA. agrove@lsu.edu
D000409:Alanine / Q000737:chemistry; D000838:Anions; D001224:Aspartic Acid / Q000737:chemistry; D001412:Bacillus subtilis / Q000201:enzymology*; D001435:Bacteriophages / Q000378:metabolism; D002412:Cations; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry*; D019281:Dimerization; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D018698:Glutamic Acid / Q000737:chemistry; D005976:Glutaral / Q000494:pharmacology; D007477:Ions; D007700:Kinetics; D008239:Lysine / Q000737:chemistry; D008958:Models, Molecular; D009154:Mutation; D010432:Pentoxyl / Q000031:analogs & derivatives* / Q000494:pharmacology; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D012492:Salts / Q000494:pharmacology*; D020124:Thermotoga maritima / Q000378:metabolism; D013941:Thymine / Q000737:chemistry; D014764:Viral Proteins
D000838:Anions; D002412:Cations; D003432:Cross-Linking Reagents; D004268:DNA-Binding Proteins; D007477:Ions; D012492:Salts; C059361:TF1 DNA-binding protein, Bacteriophage SPO1; D014764:Viral Proteins; D001224:Aspartic Acid; D018698:Glutamic Acid; C008055:5-hydroxymethyluracil; D010432:Pentoxyl; D004247:DNA; D008239:Lysine; D000409:Alanine; D013941:Thymine; D005976:Glutaral