Widespread predicted nonsense-mediated mRNA decay of alternatively-spliced transcripts of human normal and disease genes.
We have recently shown that a third of reliably-inferred alternative mRNA isoforms are candidates for nonsense-mediated mRNA decay (NMD), an mRNA surveillance system (Lewis et al., 2003; PROC: Natl Acad. Sci. USA, 100, 189-192). Rather than being translated to yield protein, these transcripts are expected to be degraded and may be subject to regulated unproductive splicing and translation (RUST). Our initial experimental studies are consistent with these predictions and suggest an unappreciated role for NMD in several human diseases.
Green
Richard E
RE
Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA.
Lewis
Benjamin P
BP
Hillman
R Tyler
RT
Blanchette
Marco
M
Lareau
Liana F
LF
Garnett
Aaron T
AT
Rio
Donald C
DC
Brenner
Steven E
SE
D017398:Alternative Splicing / Q000235:genetics*; D000544:Alzheimer Disease / Q000235:genetics; D018389:Codon, Nonsense / Q000235:genetics*; D005786:Gene Expression Regulation / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D008223:Lymphoma / Q000235:genetics; D022821:RNA Splice Sites / Q000235:genetics; D020871:RNA Stability / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
D018389:Codon, Nonsense; D022821:RNA Splice Sites; D012333:RNA, Messenger; D014157:Transcription Factors