title: A defect in the yeast plasma membrane urea transporter Dur3p is complemented by CpNIP1, a Nod26-like protein from zucchini (Cucurbita pepo L.), and by Arabidopsis thaliana delta-TIP or gamma-TIP.
abstract: Dur3 encodes the yeast plasma membrane urea transporter and Deltadur3 mutants are unable to grow on media containing low concentrations of urea as sole nitrogen source. Complementation of the Deltadur3 mutant line with expression libraries generated from whole Arabidopsis thaliana seedlings or from zucchini (Cucurbita pepo L.) vascular tissue yielded numerous lines that had regained the capacity to grow on low urea as sole nitrogen source. Analysis of several of these yeast lines revealed that the Deltadur3 mutation was complemented either by delta-TIP (TIP=tonoplast intrinsic protein) or gamma-TIP from Arabidopsis or by CpNIP1, a new NOD26-like protein from zucchini. delta-TIP (At3g16240) and gamma-TIP (At2g36830) had previously been characterized as proteins facilitating the transport of water across the tonoplast membrane, and Nod26-like proteins were characterized as glycerol transporters. So far, transport of urea has not been described for any of the proteins described in this paper. Further analyses support this function of TIPs and nodulin 26-like intrinsic proteins in urea transport.
authors.affiliation: Molekulare Pflanzenphysiologie, Universität Erlangen-Nürnberg, Staudtstrasse 5, D-91058, Erlangen, Germany.
authors.forename: Franz
authors.initials: F
authors.lastname: Klebl
authors.forename: Michael
authors.initials: M
authors.lastname: Wolf
authors.forename: Norbert
authors.initials: N
authors.lastname: Sauer
mesh_terms: D000595:Amino Acid Sequence; D020346:Aquaporins / Q000235:genetics*; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics*; D002462:Cell Membrane / Q000378:metabolism; D028464:Cucurbita / Q000235:genetics*; D005816:Genetic Complementation Test; D026901:Membrane Transport Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014508:Urea / Q000378:metabolism*
chemical_list: D020346:Aquaporins; D029681:Arabidopsis Proteins; D026901:Membrane Transport Proteins; D010940:Plant Proteins; D029701:Saccharomyces cerevisiae Proteins; C099402:delta-TIP protein, Arabidopsis; C091845:gamma-TIP protein, plant; D014508:Urea