Lipase-catalyzed synthesis of monoacylglycerol in a homogeneous system.
The 1,3-regiospecifique lipase, Lipozyme IM, catalyzed the esterification of lauric acid and glycerol in a homogeneous system. To overcome the drawback of the insolubility of glycerol in hexane, which is extensively used in enzymatic synthesis, a mixture of n-hexane/tert-butanol (1:1, v/v) was used leading to a monophasic system. The conversion of lauric acid into monolaurin was 65% in 8 h, when a molar ratio of glycerol to fatty acid (5:1) was used with the fatty acid at 0.1 M, and the phenomenon of acyl migration was minimized.
Monteiro
Julieta B
JB
Departamento de Engenharia Química e Engenharia de Alimentos, Universidade Federal de Santa Catarina, CEP 88040-900, Trindade, Florianópolis, Santa Catarina, Brazil. julieta@terra.com.br
Nascimento
Maria G
MG
Ninow
Jorge L
JL
D002384:Catalysis; D004951:Esterification; D005989:Glycerides / Q000138:chemical synthesis*; D005990:Glycerol / Q000737:chemistry*; D007848:Laurates / Q000138:chemical synthesis; D007850:Lauric Acids / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D050178:Monoglycerides; D012997:Solvents / Q000737:chemistry
D005989:Glycerides; D007848:Laurates; D007850:Lauric Acids; D050178:Monoglycerides; D012997:Solvents; C030358:lauric acid; C020777:monolaurin; C093666:Lipozyme; D008049:Lipase; D005990:Glycerol