title: "Candidatus Rickettsia tarasevichiae" in Ixodes persulcatus ticks collected in Russia.
abstract: We collected 209 Ixodes persulcatus ticks in various regions of Russia, including the southern Urals and western and eastern Siberia. Using PCR amplification and sequencing of the citrate synthase-encoding gene (gltA), we detected a new rickettsial genotype, which we named "Candidatus Rickettsia tarasevichiae." This bacterium was found in 9.27%, 10.0%, and 20.5% of the ticks collected in western Siberia, eastern Siberia, and the southern Urals, respectively. "Candidatus Rickettsia tarasevichiae" exhibited a 98% and 96% nucleotide sequence homology, with the 16S rDNA and gltA sequence, respectively, of R. canadensis, a rickettsia previously only found in Haemaphysalis leporispalustris ticks in North America. The phylogenetic analysis of "Candidatus Rickettsia tarasevichiae" and other Rickettsia species allowed the creation of a new cluster with high bootstrap values within the Rickettsia genus involving this rickettsia, R. canadensis, and three uncultured rickettsiae from plant insects.
authors.affiliation: Omsk Research Institute of Natural Infectious Foci, Omsk, Russia.
authors.forename: Stanislav
authors.initials: S
authors.lastname: Shpynov
authors.forename: Pierre-Edouard
authors.initials: PE
authors.lastname: Fournier
authors.forename: Nikolay
authors.initials: N
authors.lastname: Rudakov
authors.forename: Didier
authors.initials: D
authors.lastname: Raoult
mesh_terms: D000818:Animals; D001483:Base Sequence; D002950:Citrate (si)-Synthase / Q000235:genetics; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005838:Genotype; D005843:Geography; D018884:Ixodes / Q000382:microbiology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012281:Rickettsia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012426:Russia; D012800:Siberia
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D002950:Citrate (si)-Synthase