Antibody to CD14 like CXCR4-specific antibody 12G5 could inhibit CXCR4-dependent chemotaxis and HIV Env-mediated cell fusion.
The expression of HIV-1 coreceptors (CXCR4 and CCR5) on monocyte surface can be regulated by the ligand of CD14 (LPS), which stimulate the susceptibility of the cells to HIV-1. To investigate whether it exists potential association between CD14 and HIV-1 coreceptor CXCR4, we tested the impact of CD14-specific monoclonal antibodies (mAbs) upon CXCR4-dependent responses, such as SDF-induced chemotaxis and HIV Env-mediated membrane fusion. The anti-CD14 mAb TUK4 like CXCR4-specific mAb 12G5 could block SDF-induced chemotaxis of U937 cells in a dose-dependent manner, while another CD14-specific mAb UCHM-1 did not show any activity. More interestingly, syncytium assay indicated that only the CD14-specific mAb TUK4 inhibited HIV Env-mediated CXCR4-dependent cell fusion between U937 cells and HIV-1(HXB2) Env transfected CHO cells distinctly, consistent with its activity against CXCR4-dependent chemotaxis. These results provided experimental evidence for existence of close association between CD14 and HIV coreceptor CXCR4 on human monocytic cells.
Yang
Hengwen
H
Laboratory of Immunology, Research Centre for Medical Science and Department of Biological Science and Technology, Tsinghua University; Protein Science, Laboratory of the Ministry of Education, Beijing, 100084, PR China.
Lan
Canhui
C
Xiao
Yi
Y
Chen
Ying-Hua
YH
D016475:3T3 Cells; D000818:Animals; D000911:Antibodies, Monoclonal; D016466:CHO Cells; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte; D006224:Cricetinae; D003412:Cricetulus; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000276:immunology / Q000502:physiology*; D008561:Membrane Fusion; D051379:Mice; D009000:Monocytes / Q000502:physiology*; D019718:Receptors, CXCR4 / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D015728:Receptors, HIV / Q000276:immunology / Q000502:physiology*; D014162:Transfection; D020298:U937 Cells
D000911:Antibodies, Monoclonal; D018950:Lipopolysaccharide Receptors; D019718:Receptors, CXCR4; D015728:Receptors, HIV