title: The effect of lumbrokinase on P-selectin and E-selectin in cerebral ischemia model of rat.
abstract: OBJECTIVE
  To find the effect of lumbrokinase (LK) on P-selectin and E-selectin in ischemic rats.
  
  
  METHODS
  Male healthy Spragur-Dawley rats weighing 180-220 g (n = 90) were divided into 4 groups: (1) normal control group (n = 5), (2) sham-operated group (n = 35), (3) ischemic group (n = 35), (4) LK group (n = 15). LK 10 mg/kg (2000 UK activity of LK) was given by intraperitoneal injection in the LK group 30 minutes before experiment. Same volume of normal saline was given in the sham-operated group and ischemic group. The ischemic model was made by modified Haruo Nagasawa's method. Immunohistochemistry was used to observe the P-selectin and E-selectin positive cells in the ischemic region.
  
  
  RESULTS
  P-selectin and E-selectin positive cells in ischemic regions were observed in the ischemic group, and the peak of expression was at 6 hours and 12 hours, respectively. The similar changes were not observed in normal control group. There were only a few positive cells in the sham-operated group. In LK group, the P-selectin and E-selectin positive cells were significantly less than those in the ischemic group (P < 0.05 at 3 hours after the onset, P < 0.01 at 6 hours and P < 0.01 at 12 hours, respectively).
  
  
  CONCLUSIONS
  LK might significantly decrease the immunoreactions of P-selectin and E-selectin in ischemic lesion.
authors.affiliation: Department of Neurology, PLA261 Hospital, Beijing, 100094.
authors.forename: Xiaoshu
authors.initials: X
authors.lastname: Zhang
authors.forename: Jiatang
authors.initials: J
authors.lastname: Zhang
authors.forename: Peizi
authors.initials: P
authors.lastname: Kuang
authors.forename: Senyang
authors.initials: S
authors.lastname: Lang
authors.forename: Weiping
authors.initials: W
authors.lastname: Wu
authors.forename: Yumin
authors.initials: Y
authors.lastname: Yuan
authors.forename: Jiexiao
authors.initials: J
authors.lastname: Liu
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.forename: Peigen
authors.initials: P
authors.lastname: Kuang
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism*; D015536:Down-Regulation; D019040:E-Selectin / Q000378:metabolism*; D010450:Endopeptidases / Q000494:pharmacology*; D008297:Male; D019007:P-Selectin / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D019040:E-Selectin; D019007:P-Selectin; D010450:Endopeptidases; C083421:lumbrokinase