Maleic anhydride copolymers--a versatile platform for molecular biosurface engineering.
A platform of thin polymer coatings was introduced for the functional modulation of immobilized bioactive molecules at solid/liquid interfaces. The approach is based on covalently attached alternating maleic acid anhydride copolymers with a variety of comonomers and extended through conversion of the anhydride moieties by hydrolysis, reaction with functional amines, and other conversions of the anhydride moieties. We demonstrate that these options permit control of the physicochemical constraints for bioactive molecules immobilized at interfaces to influence important performance characteristics of biofunctionalized materials for medical devices and molecular diagnostics. Examples concern the impact of the substrate-anchorage of fibronectin on the formation of cell-matrix adhesions, the orientation of endothelial cells according to lateral anti-adhesive micropatterns using grafted poly(ethylene oxide), and the spacer-dependent activity of immobilized synthetic thrombin inhibitors.
Pompe
Tilo
T
Institute of Polymer Research Dresden and The Max Bergmann Center of Biomaterials Dresden, Hohe Str. 6, 01069 Dresden, Germany.
Zschoche
Stefan
S
Herold
Nicole
N
Salchert
Katrin
K
Gouzy
Marie-Francoise
MF
Sperling
Claudia
C
Werner
Carsten
C
D000588:Amines / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry; D001698:Biomedical Engineering / Q000379:methods*; D002448:Cell Adhesion / Q000187:drug effects; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects; D005353:Fibronectins / Q000737:chemistry; D006801:Humans; D006868:Hydrolysis; D008299:Maleic Anhydrides / Q000737:chemistry*; D015394:Molecular Structure; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D013499:Surface Properties
D000588:Amines; D001672:Biocompatible Materials; D005353:Fibronectins; D008299:Maleic Anhydrides; D011108:Polymers; C016683:polymaleic anhydride; D011092:Polyethylene Glycols