title: Selective enrichment of Geobacter sulfurreducens from anaerobic granular sludge with quinones as terminal electron acceptors.
abstract: A quinone-respiring, enrichment culture derived from methanogenic granular sludge was phylogenetically characterized by using a combined cloning-denaturing gradient gel electrophoresis (DGGE) method, which revealed that the consortium developed was dominated by a single microorganism: 97% related, in a sequence of 1520 base pairs, to Geobacter sulfurreducens. The enrichment culture could grow with acetate, formate or H2 when humic acids, the humic model compound, anthraquinone-2,6-disulfonate (AQDS), or chelated Fe(III) was provided as a terminal electron acceptor. The occurrence of a humic acid- or quinone-respiring microorganism in the microbial community of a wastewater treatment system suggests that this type of microorganisms may play a potential role in anaerobic bioreactors treating humus-containing wastewaters.
authors.affiliation: Sub-Department of Environmental Technology, Wageningen University, P.O. Box 8129, 6700 EV Wageningen, The Netherlands. fcervantes@yaqui.itson.mx
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Cervantes
authors.forename: Tuan
authors.initials: T
authors.lastname: Duong-Dac
authors.forename: Anna E
authors.initials: AE
authors.lastname: Ivanova
authors.forename: Kees
authors.initials: K
authors.lastname: Roest
authors.forename: Antoon D L
authors.initials: AD
authors.lastname: Akkermans
authors.forename: Gatze
authors.initials: G
authors.lastname: Lettinga
authors.forename: Jim A
authors.initials: JA
authors.lastname: Field
mesh_terms: D000085:Acetates / Q000378:metabolism*; D000693:Anaerobiosis; D000880:Anthraquinones / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods; D020552:Comet Assay / Q000379:methods; D003470:Culture Media / Q000378:metabolism; D020564:Deltaproteobacteria / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism* / Q000502:physiology; D004579:Electron Transport; D005561:Formates / Q000378:metabolism; D006812:Humic Substances / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism; D010084:Oxidation-Reduction; D010802:Phylogeny; D011809:Quinones / Q000378:metabolism; D016415:Sequence Alignment / Q000379:methods; D017421:Sequence Analysis / Q000379:methods; D012722:Sewage / Q000382:microbiology*; D012988:Soil Microbiology; D013379:Substrate Specificity
chemical_list: D000085:Acetates; D000880:Anthraquinones; D003470:Culture Media; D005561:Formates; D006812:Humic Substances; D011809:Quinones; D012722:Sewage; C408124:anthraquinone-2,6-disulfonate; C030544:formic acid; D006859:Hydrogen