Two new peptides to improve post-operative gastric ileus in dog.
Peptides can influence gastrointestinal motility, and from data obtained earlier in rats, we hypothesized that MTL-RP/Ghrelin, as well as CGRP receptor antagonist 8-37, could improve gastric post-operative ileus in dog. Dogs submitted to laparotomy were perfused with or saline or CGRP 8-37 or MTL-RP/Ghrelin on days 1-4 post-operatively while gastric emptying was estimated by measuring the postprandial increase in plasma acetaminophen ingested with a meal. As expected, in saline-treated animals the gastric emptying function was impaired post-operatively. The total amount of acetaminophen emptied (AUC over 150 min) on post-operative days 1-4 reached respectively 31+/-5%, 65+/-8%, 60+/-8% and 62+/-8% of the normal emptying capacity. CGRP antagonist increased the total emptying of acetaminophen to 52+/-5% on day 1, 95+/-2% on day 2 and 103+/-3% (P<0.05) on day 3. The delayed emptying of acetaminophen seen post-operatively in saline-treated animals could be completely reversed by MTL-RP/Ghrelin (P<0.01) whether it was given at 100 microg/kg on day 2 (102+/-7% of the normal emptying capacity), 4 microg/kg on day 3 (106+/-7%) or 20 microg/kg on day 4 (132+/-8%). As found earlier in rodents, CGRP receptor antagonist 8-37 as well as MTL-RP/Ghrelin are potent prokinetics to improve post-operative gastric ileus in dog.
Trudel
L
L
Centre hospitalier de l'Université de Montréal, Hôpital Saint-Luc, Montreal, Que., Canada.
Bouin
M
M
Tomasetto
C
C
Eberling
P
P
St-Pierre
S
S
Bannon
P
P
L'Heureux
M C
MC
Poitras
P
P
D000082:Acetaminophen / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D000818:Animals; D019540:Area Under Curve; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology; D004285:Dogs; D005260:Female; D005746:Gastric Emptying; D054439:Ghrelin; D007082:Ileum / Q000473:pathology*; D045823:Ileus / Q000378:metabolism; D007700:Kinetics; D010446:Peptide Fragments / Q000494:pharmacology; D036361:Peptide Hormones / Q000494:pharmacology; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D011183:Postoperative Complications; D019518:Postprandial Period; D013270:Stomach / Q000473:pathology*; D013997:Time Factors
D054439:Ghrelin; D010446:Peptide Fragments; D036361:Peptide Hormones; D010455:Peptides; C058636:calcitonin gene-related peptide (8-37); D000082:Acetaminophen; D015740:Calcitonin Gene-Related Peptide