title: Determination of dopamine in single rat pheochromocytoma cell by capillary electrophoresis with amperometric detection.
abstract: A method is described for the direct identification of dopamine in single cultured rat pheochromocytoma cell by capillary electrophoresis (CE) with amperometric detection. The separation and detection conditions were optimized. The dopamine in single cell analysis was identified based on the migration time of standard dopamine and internal standard (epinephrine). The amount of dopamine in a single cell ranged from 0.29 to 1.28 fmol.
authors.affiliation: Department of Chemistry, Wuhan University, Wuhan 430072, China.
authors.forename: Liyao
authors.initials: L
authors.lastname: Zhang
authors.forename: Sanfu
authors.initials: S
authors.lastname: Qv
authors.forename: Zongli
authors.initials: Z
authors.lastname: Wang
authors.forename: Jieke
authors.initials: J
authors.lastname: Cheng
mesh_terms: D000818:Animals; D004298:Dopamine / Q000032:analysis*; D004563:Electrochemistry / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D016716:PC12 Cells; D010673:Pheochromocytoma / Q000737:chemistry*; D051381:Rats; D012015:Reference Standards; D014407:Tumor Cells, Cultured
chemical_list: D004298:Dopamine