Poly(hydroxybutyrate-co-hydroxyhexanoate) promoted production of extracellular matrix of articular cartilage chondrocytes in vitro.
The present investigation describes the production of extracellular matrix of rabbit articular cartilage chondrocytes grown on scaffolds of polyhydroxybutyrate (PHB) blended with poly(hydroxybutyrate-co-hydroxyhexanoate) (PHBHHx) for up to 7 days. The mRNA level of type II collagen of chondrocytes seeded on all scaffolds consisting of PHBHHx were obviously higher than that of PHB-only scaffold throughout the culture period, suggesting the positive effect of PHBHHx on extracellular matrix production. Second-harmonic generation (SHG) imaging technique, combined with confocal fluorescence microscopy (CFM) revealed that PHBHHx in PHB scaffold provided better surface properties for anchoring type II collagen filaments and their penetration into internal layers of the scaffolds. Glycosaminoglycan (GAG), a major composition of extracellular matrix, showed a sharp increase in construct of 1:2 PHB/PHBHHx scaffold after 7 day cultivation, while only a small increase was observed in all other tested scaffolds. At the same time, total collagen contents in all scaffolds containing PHBHHx increased with time, with the maximum collagen production of 742.1+/-99.2mg/g dry weight observed in construct of 1:2 PHB/PHBHHx scaffold inoculated for 7 days, this was almost 4-fold higher than that in scaffold of PHB only. It appears that the presence of right proportion of PHBHHx in the composite system of PHB/PHBHHx highly favored the production of extracellular matrix of articular cartilage chondrocytes.
Deng
Ying
Y
Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China.
Lin
Xing-sun
XS
Zheng
Zhong
Z
Deng
Jin-Guang
JG
Chen
Jin-Chun
JC
Ma
Hui
H
Chen
Guo-Qiang
GQ
D020155:3-Hydroxybutyric Acid / Q000737:chemistry* / Q000494:pharmacology; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002208:Caproates / Q000737:chemistry* / Q000494:pharmacology; D002356:Cartilage / Q000378:metabolism; D002358:Cartilage, Articular / Q000737:chemistry / Q000378:metabolism*; D019902:Chondrocytes / Q000378:metabolism*; D003094:Collagen / Q000737:chemistry; D024043:Collagen Type II / Q000737:chemistry; D005109:Extracellular Matrix / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000737:chemistry; D006885:Hydroxybutyrates / Q000737:chemistry; D007091:Image Processing, Computer-Assisted; D066298:In Vitro Techniques; D018613:Microscopy, Confocal; D011091:Polyesters / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D000094122:Polyhydroxybutyrates
D020155:3-Hydroxybutyric Acid; D001672:Biocompatible Materials; D002208:Caproates; D003094:Collagen; D024043:Collagen Type II; D006025:Glycosaminoglycans; D006885:Hydroxybutyrates; D011091:Polyesters; D011108:Polymers; D012333:RNA, Messenger; C115940:poly(3-hydroxybutyrate-co-3-hydroxyhexanoate); D000094122:Polyhydroxybutyrates; C003182:poly-beta-hydroxybutyrate