title: Monocyte activation markers during cardiopulmonary bypass.
abstract: Extracorporeal support during cardiac surgery initiates an inflammatory response, causing damage to cardiac, pulmonary and renal tissue [Post Pump Syndrome (PPS)]. This is accompanied by a neutrophil leucocytosis and lymphopenia, but less is known about the role of monocytes and markers of monocyte activity. We studied 19 patients undergoing cardiac surgery, obtaining blood samples from the aortic root (AR) and from the coronary sinus ( < s) before the cardiopulmonary bypass (CPB), 1 min after release of the aortic crossclamp and 10 min after weaning from CPB (periods 1, 2 and 3). Leucocyte count, monocyte count and HLA-DR, CD15, CD11b and CD62L activation markers were measured. In samples obtained from the coronary sinus (CS), HLA-DR, expressed as a percentage of the monocyte count, decreased between periods 1, 2 and 3 by 78%, 66% and 43%, respectively. A similar change was observed in samples from the AR. Conversely, CD62L increased in the CS samples (55%, 68% and 73%), but revealed a lesser increase in the AR samples (51%, 68% and 63%). The other markers showed little change throughout the procedure. Reduced immunological competence could result from the decrease in HLA-DR counts. Increases in CD62L sensitizes monocytes to the tethering effects of endothelial integrins and might contribute to the atherosclerotic process.
authors.affiliation: Department of Pathology, West Middlesex University Hospital, Isleworth, UK. Richard.Fink@wmuh-tr.nthames.nhs.uk
authors.forename: R
authors.initials: R
authors.lastname: Fink
authors.forename: M
authors.initials: M
authors.lastname: Al-Obaidi
authors.forename: S
authors.initials: S
authors.lastname: Grewal
authors.forename: M
authors.initials: M
authors.lastname: Winter
authors.forename: J
authors.initials: J
authors.lastname: Pepper
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001021:Aortic Valve / Q000601:surgery; D015415:Biomarkers / Q000032:analysis; D039481:CD11b Antigen / Q000032:analysis; D002315:Cardiopulmonary Bypass / Q000009:adverse effects*; D001026:Coronary Artery Bypass / Q000009:adverse effects; D005260:Female; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007249:Inflammation / Q000097:blood*; D019041:L-Selectin / Q000032:analysis; D007958:Leukocyte Count; D016256:Lewis X Antigen / Q000032:analysis; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism / Q000502:physiology
chemical_list: D015415:Biomarkers; D039481:CD11b Antigen; D006684:HLA-DR Antigens; D016256:Lewis X Antigen; D019041:L-Selectin