Glucocorticoid maintains pulsatile aecretion of luteinizing hormone under infectious stress condition.
We have previously shown that TNF-alpha, a major proinflammatory cytokine, suppressed hypothalamic GnRH pulse generator activity and that this inhibitory effect was enhanced by alpha-helical CRH, a CRH receptor antagonist. The present study was conducted to elucidate the involvement of glucocorticoid (GC) in modulating LH pulses under infectious stress condition. Adrenalectomy (ADX) markedly enhanced the suppressive effect of TNF-alpha (1 micro g), injected iv, on LH pulses in ovariectomized (OVX) rats. Pretreatment with a sc injection of corticosterone (10 mg) almost completely restored LH pulses after TNF-alpha injection in OVX/ADX animals. Injection of TNF-alpha increased the number of c-Fos-immunoreactive cells in the supraoptic nucleus (SON), the dorsomedial hypothalamic nucleus (DMH), and the parvocellular region of the paraventricular nucleus (PVN), which was more prominent in OVX/ADX than OVX animals except in the DMH. Pretreatment with corticosterone decreased the number of Fos-immunoreactive cells in the PVN and SON but not in the DMH. These results suggest that GC has a potent protective effect on LH pulsatility under conditions of infectious stress, the mechanism of which involves at least the suppression of the excitability of PVN and SON neurons. In addition, the DMH does not seem to mediate the central action of GC, though it may play an important role in inducing pathophysiological reactions to invasive stress.
Matsuwaki
Takashi
T
Department of Veterinary Physiology, Veterinary Medical Science, The University of Tokyo, Tokyo 113-8657, Japan.
Watanabe
Erina
E
Suzuki
Masatoshi
M
Yamanouchi
Keitaro
K
Nishihara
Masugi
M
D000315:Adrenalectomy; D000818:Animals; D003345:Corticosterone / Q000494:pharmacology*; D004302:Dorsomedial Hypothalamic Nucleus / Q000737:chemistry; D005260:Female; D007239:Infections / Q000503:physiopathology*; D007986:Luteinizing Hormone / Q000378:metabolism*; D010052:Ovariectomy; D010286:Paraventricular Hypothalamic Nucleus / Q000737:chemistry; D010507:Periodicity; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D013312:Stress, Physiological / Q000503:physiopathology*; D013495:Supraoptic Nucleus / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D016760:Proto-Oncogene Proteins c-fos; D014409:Tumor Necrosis Factor-alpha; D007986:Luteinizing Hormone; D003345:Corticosterone