[Analysis of correlation between parameters of sperm chromatin structure assay and semen routine analysis].
OBJECTIVE
To analysis the correlation between parameters of sperm chromatin structure assay(SCSA) and semen routine analysis, and to discuss the reliable methods of semen quality evaluation.


METHODS
Five hundred and eleven semen samples were detected to analyse the mutiple-parameter correlation between results of SCSA (COMP alpha t) and semen routine analysis.


RESULTS
The parameters that have low-level positive correlation(r: 0.10-0.30) with denatured sperm percentage(COMP alpha t) were viscosity, ejaculation interval, abnormal sperm ratio, concentration of grade c sperm; those having low-level negative correlation(r: -0.30(-)-0.10) were VDL, VSL and VAP; those having mid-level positive correlation (r: 0.30-0.70) were sperm concentration, percentage of grade d sperm; those having mid-level negative correlation (r: -0.70(-)-0.30) were MAD, percentage of grade a sperm and survival rate.


CONCLUSIONS
Flow cytometry can be used to evaluate the percentage of denatured or injured sperm rapidly, correctly and simply. The result (COMP alpha t) correlates partly with semen parameters, and it is not a conclusively parameter compared with routine semen analysis. It is important to use SCSA to evaluate productivity under the above situation.
Zhang
Ning
N
Reproduction Centre, 202 Hospital of PLA, Shenyang, Liaoning 110003, China.
Zhang
Li
L
Ding
Xiao-Ping
XP
Lu
Hai-Ou
HO
Tang
Jie
J
D000328:Adult; D002843:Chromatin / Q000737:chemistry*; D004249:DNA Damage; D005434:Flow Cytometry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D012661:Semen; D013076:Sperm Count; D013094:Spermatozoa / Q000648:ultrastructure*; D014783:Viscosity
D002843:Chromatin