A chloride-permeable channel from Phaseolus vulgaris roots incorporated into planar lipid bilayers.
Ion channels are key participants in physiological processes of plant cells. Here, we report the first characterization of a high conductance, Cl(-)-permeable channel, present in enriched fractions of plasma membranes of bean root cells. The Cl(-) channel was incorporated into planar lipid bilayers and its activity was recorded under voltage clamp conditions. The channel is voltage-dependent, excludes the passage of cations (K(+), Na(+), and Ca(2+)), and is inhibited by micromolar concentrations of Zn(2+). The Cl(-) conductance here characterized represents a previously undescribed channel of plant cells.
Balleza
Daniel
D
Departamento de Biologi;a Molecular de Plantas, Instituto de Biotecnologi;a, UNAM, Cuernavaca Morelos 62210, Mexico.
Quinto
Carmen
C
Sánchez
Federico
F
Gómez-Lagunas
Froylan
F
D002462:Cell Membrane / Q000378:metabolism; D018118:Chloride Channels / Q000378:metabolism*; D015640:Ion Channel Gating; D008051:Lipid Bilayers / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D027805:Phaseolus / Q000033:anatomy & histology / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism
D018118:Chloride Channels; D008051:Lipid Bilayers; D010940:Plant Proteins; D015032:Zinc