Experimental envenoming of mice with venom from the scorpion Centruroides limpidus limpidus: differences in mortality and symptoms with and without antibody therapy relating to differences in age, sex and strain of mouse.
C57Bl/6J and BALB/cAnN inbred strains of mice differed significantly in mortality and symptoms when intoxicated subcutaneously with one LD(50) of venom from Centruroides limpidus limpidus. Higher mortality was observed in C57Bl/6J than in BALB/cAnN. Also, C57Bl/6J mice more quickly developed muscular and respiratory collapse whilst BALB/cAnN mice were hyperactive before dying. Also, the symptoms in the survivors lasted for 24 h in C57Bl/6J and for 2 h in BALB/cAnN. The age and sex of mice were also related to mortality: younger mice were more resistant than older mice and females were more susceptible than males, especially in the younger groups. Antivenom (horse F(ab')(2)) administration 5-10 min after envenoming of mice with one LD(50) rescued 60% of BALB/cAnN and 52% of C57Bl/6J mice, respectively. Results indicate that genetic background, gender and age differences are of consequence in the pathogenesis of C. limpidus scorpion envenomation in mice, and that timely treatment with active antivenom F(ab')(2) saves a significant fraction of intoxicated mice without statistically significant distinction of strains.
Padilla
Alejandro
A
Departamento de Microbiologi;a y Parasitología, Unidad de Medicina Experimental Facultad de Medicina, UNAM, Hospital General de México, Dr Balmis 148, Colonia Doctores, 06726, México DF, Mexico.
Govezensky
Tzipe
T
Possani
Lourival D
LD
Larralde
Carlos
C
D000367:Age Factors; D017109:Akathisia, Drug-Induced / Q000209:etiology; D000818:Animals; D000931:Antidotes / Q000494:pharmacology; D005260:Female; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009498:Neurotoxins / Q000633:toxicity*; D011595:Psychomotor Agitation / Q000209:etiology; D012119:Respiration / Q000187:drug effects; D012604:Scorpion Venoms / Q000633:toxicity*; D012737:Sex Factors; D018675:Toxicity Tests
D000931:Antidotes; C035413:Centruroides toxin; D009498:Neurotoxins; D012604:Scorpion Venoms