T1alpha/podoplanin deficiency disrupts normal lymphatic vasculature formation and causes lymphedema.
Within the vascular system, the mucin-type transmembrane glycoprotein T1alpha/podoplanin is predominantly expressed by lymphatic endothelium, and recent studies have shown that it is regulated by the lymphatic-specific homeobox gene Prox1. In this study, we examined the role of T1alpha/podoplanin in vascular development and the effects of gene disruption in mice. T1alpha/podoplanin is first expressed at around E11.0 in Prox1-positive lymphatic progenitor cells, with predominant localization in the luminal plasma membrane of lymphatic endothelial cells during later development. T1alpha/podoplanin(-/-) mice die at birth due to respiratory failure and have defects in lymphatic, but not blood vessel pattern formation. These defects are associated with diminished lymphatic transport, congenital lymphedema and dilation of lymphatic vessels. T1alpha/podoplanin is also expressed in the basal epidermis of newborn wild-type mice, but gene disruption did not alter epidermal differentiation. Studies in cultured endothelial cells indicate that T1alpha/podoplanin promotes cell adhesion, migration and tube formation, whereas small interfering RNA-mediated inhibition of T1alpha/podoplanin expression decreased lymphatic endothelial cell adhesion. These data identify T1alpha/podoplanin as a novel critical player that regulates different key aspects of lymphatic vasculature formation.
Schacht
Vivien
V
Cutaneous Biology Research Center, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA.
Ramirez
Maria I
MI
Hong
Young-Kwon
YK
Hirakawa
Satoshi
S
Feng
Dian
D
Harvey
Natasha
N
Williams
Mary
M
Dvorak
Ann M
AM
Dvorak
Harold F
HF
Oliver
Guillermo
G
Detmar
Michael
M
D000818:Animals; D000831:Animals, Newborn; D002448:Cell Adhesion; D002465:Cell Movement; D002478:Cells, Cultured; D004729:Endothelium, Lymphatic / Q000166:cytology / Q000196:embryology / Q000378:metabolism / Q000648:ultrastructure; D018507:Gene Expression Regulation, Developmental; D006023:Glycoproteins / Q000378:metabolism / Q000648:ultrastructure; D018398:Homeodomain Proteins / Q000378:metabolism / Q000648:ultrastructure; D008208:Lymphatic System / Q000098:blood supply / Q000166:cytology / Q000196:embryology* / Q000473:pathology; D008209:Lymphedema / Q000209:etiology* / Q000473:pathology; D008562:Membrane Glycoproteins / Q000172:deficiency* / Q000378:metabolism / Q000648:ultrastructure; D008565:Membrane Proteins / Q000172:deficiency / Q000378:metabolism / Q000502:physiology*; D026901:Membrane Transport Proteins; D051379:Mice; D018345:Mice, Knockout; D018919:Neovascularization, Physiologic / Q000235:genetics; D034741:RNA, Small Interfering / Q000378:metabolism; D025521:Tumor Suppressor Proteins; D000098551:Prospero-Related Homeobox 1 Protein; D000098853:Podoplanin
D006023:Glycoproteins; D018398:Homeodomain Proteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D034741:RNA, Small Interfering; D025521:Tumor Suppressor Proteins; D000098551:Prospero-Related Homeobox 1 Protein; C490441:Pdpn protein, mouse; D000098853:Podoplanin; C117468:PDPN protein, human; C492273:LYVE1 protein, mouse