Using a DNA microarray to investigate the distribution of insect virulence factors in strains of photorhabdus bacteria.
Photorhabdus is an insect-pathogenic bacterium in which oral toxicity to insects is found in two distinct taxonomic groups. Using a DNA microarray and comparative genomics, we show that oral toxicity is associated with toxin complex genes tcaABC and that this locus can be mobilized or deleted within different strains.
Marokhazi
Judit
J
Department of Biology and Biochemistry, University of Bath, Bath, BA2 7AY, United Kingdom.
Waterfield
Nicholas
N
LeGoff
Gaelle
G
Feil
Edward
E
Stabler
Richard
R
Hinds
Jason
J
Fodor
Andras
A
ffrench-Constant
Richard H
RH
D000284:Administration, Oral; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D007313:Insecta / Q000382:microbiology*; D009348:Nematoda / Q000382:microbiology; D020411:Oligonucleotide Array Sequence Analysis; D020637:Photorhabdus / Q000145:classification / Q000235:genetics / Q000472:pathogenicity*; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*; D020593:Xenorhabdus / Q000145:classification / Q000235:genetics / Q000472:pathogenicity
D001426:Bacterial Proteins; D037521:Virulence Factors