title: Molecular cloning and characterization of rKAB1, which interacts with KARP-1, localizes in the nucleus and protects cells against oxidative death.
abstract: The Ku autoantigen/KARP-1 (Ku86 autoantigen related protein-1) plays an important role in the double-strand break repair of mammalian DNA as a DNA-binding component of DNA-dependent protein kinase (DNA-PK) complex. KARP-1 is differently transcribed from the human Ku86 autoantigen gene locus and it is implicated in the control of DNA-dependent protein kinase activity. We cloned rKAB1, a rat homolog of KAB1 (KARP-1 binding protein 1 of human) from a rat hippocampal cDNA library. rKAB1 mRNA was specifically expressed in the brain and the thymus. EGFP-tagged rKAB1 protein localized in cell nucleus and in the condensed chromosome during the mitotic cell division. We found that rKAB1 works as a protective protein against cell damage by oxidative stress.
authors.affiliation: Department of Pharmacology, Osaka University Medical School, Suita, Osaka, Japan.
authors.forename: Eunju
authors.initials: E
authors.lastname: Do
authors.forename: Eiichi
authors.initials: E
authors.lastname: Taira
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Irie
authors.forename: Yehua
authors.initials: Y
authors.lastname: Gan
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Tanaka
authors.forename: Che-Hui
authors.initials: CH
authors.lastname: Kuo
authors.forename: Naomasa
authors.initials: N
authors.lastname: Miki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D034961:Antigens, Nuclear / Q000096:biosynthesis; D015152:Blotting, Northern; D001921:Brain / Q000378:metabolism; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D016923:Cell Death; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D002470:Cell Survival; D002875:Chromosomes / Q000648:ultrastructure; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D004265:DNA Helicases; D004260:DNA Repair; D051747:DNA-Activated Protein Kinase; D004268:DNA-Binding Proteins / Q000096:biosynthesis; D015723:Gene Library; D006367:HeLa Cells; D006624:Hippocampus / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D000072200:Ku Autoantigen; D051379:Mice; D008969:Molecular Sequence Data; D041681:NIH 3T3 Cells; D009687:Nuclear Proteins; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism*; D011233:Precipitin Tests; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D013950:Thymus Gland / Q000378:metabolism; D014018:Tissue Distribution; D014162:Transfection
chemical_list: D034961:Antigens, Nuclear; D002352:Carrier Proteins; C483399:Cep170 protein, rat; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D004247:DNA; D006861:Hydrogen Peroxide; D051747:DNA-Activated Protein Kinase; C497263:PRKDC protein, human; D017346:Protein Serine-Threonine Kinases; D004265:DNA Helicases; C106893:XRCC5 protein, human; C000606512:Xrcc5 protein, mouse; C000606509:Xrcc6 protein, human; C000606510:Xrcc6 protein, mouse; C000606511:Xrcc6 protein, rat; D000072200:Ku Autoantigen; D010100:Oxygen