title: Increased tissue oxygen extraction and acidosis with progressive severity of sepsis.
abstract: BACKGROUND
  Lactic acidosis and increased production of CO(2) are common in septic shock. Presumably, both acidosis and CO(2) enhance the release of oxygen from hemoglobin. The purpose of this study was to assess the relationship of oxygen utilization, CO(2) production, acidosis, and hemoglobin oxygen (Hgb-O(2)) dissociation with progressive severity of sepsis to shock.
  
  
  METHODS
  Femoral arterial and vein, hepatic vein, portal vein, and pulmonary artery catheters were placed in 16 anesthetized swine. Organ blood flow was determined by timed injections of colored microspheres. After baseline measurements, Pseudomonas aeruginosa was infused in eight animals. This bacterial slurry was continued inciting a progression of sepsis to shock. Eight animals served as instrumented controls.
  
  
  RESULTS
  With sepsis and shock, there was a progressive decrease in pH and an increase in pCO(2) in plasma with all sampling sites (P < 0.01 septic shock versus baseline versus control). Blood flow to the liver and intestines increased with sepsis (P < 0.01) but then returned to near baseline control values during shock. VO(2) and/or percent O(2) extraction increased with sepsis and septic shock for the whole body and for the liver, intestine and leg (P < 0.01). There was a strong correlation between venous O(2) saturation, acidosis, and pCO(2) to percent O(2) extraction (r > 60; P < 0.0001). However, calculated P(50) values for Hgb-O(2) dissociation remained unchanged.
  
  
  CONCLUSIONS
  This study demonstrates that increased oxygen extraction in severe sepsis is related to a fall in tissue oxygen availability and not related to any allosteric change in Hgb-O(2) dissociation. Therefore, acidosis and hypercapnia do not have a demonstrable effect on altering oxygen availability during sepsis.
authors.affiliation: Department of Surgery, The University of Texas Medical Branch, Galveston, Texas 77555-1172, USA.
authors.forename: David W
authors.initials: DW
authors.lastname: Hart
authors.forename: David L
authors.initials: DL
authors.lastname: Chinkes
authors.forename: Dennis C
authors.initials: DC
authors.lastname: Gore
mesh_terms: D000136:Acid-Base Equilibrium; D000138:Acidosis / Q000378:metabolism*; D000818:Animals; D001794:Blood Pressure; D002245:Carbon Dioxide / Q000378:metabolism; D018450:Disease Progression; D006339:Heart Rate; D007413:Intestinal Mucosa / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D010101:Oxygen Consumption / Q000502:physiology*; D010108:Oxyhemoglobins / Q000378:metabolism; D018805:Sepsis / Q000378:metabolism*; D012720:Severity of Illness Index; D013552:Swine
chemical_list: D010108:Oxyhemoglobins; D002245:Carbon Dioxide; D019344:Lactic Acid