Tachykinin receptor (NK(1), NK(2), NK(3)) binding sites in the rat caudal brainstem following neonatal capsaicin administration.
Binding of [125I]-Bolton-Hunter substance P ([125I]-BHSP), [125I]-neurokinin A and [3H]-senktide to tachykinin NK(1), NK(2) and NK(3) receptors, respectively, was examined in caudal brainstem sections of 10-week-old rats pretreated as neonates (P2) with capsaicin (50 mg/kg, s.c.) or vehicle. [125I]-BHSP binding was localised to the nucleus of the solitary tract (NTS), hypoglossal nucleus and inferior olivary complex, whereas [125I]-neurokinin A and [3H]-senktide binding were confined to the NTS. The distribution and density of binding sites were similar in vehicle- and capsaicin-pretreated rats, suggesting that sensory neuron ablation by neonatal capsaicin does not affect tachykinin receptor numbers in the rat caudal brainstem.
Geraghty
Dominic P
DP
School of Human Life Sciences, University of Tasmania, Locked bag 1320, Tasmania 7250, Launceston, Australia. d.geraghty@utas.edu.au
Mazzone
Stuart B
SB
D000818:Animals; D000831:Animals, Newborn; D001345:Autoradiography; D001933:Brain Stem / Q000187:drug effects* / Q000378:metabolism; D002211:Capsaicin / Q000494:pharmacology*; D007202:Indicators and Reagents / Q000494:pharmacology; D007457:Iodine Radioisotopes / Q000494:pharmacology; D015288:Neurokinin A / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology; D051381:Rats; D018039:Receptors, Tachykinin / Q000187:drug effects* / Q000378:metabolism; D013373:Substance P / Q000031:analogs & derivatives* / Q000494:pharmacology; D013388:Succinimides / Q000494:pharmacology
D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D010446:Peptide Fragments; D018039:Receptors, Tachykinin; D013388:Succinimides; C054724:senktide; D013373:Substance P; C022581:Bolton-Hunter reagent; D015288:Neurokinin A; D002211:Capsaicin