title: Expression of 5-HT2B and 5-HT2C receptor genes is associated with proliferative regions of Xenopus developing brain and eye.
abstract: Here we clone the Xenopus 5-HT2B receptor cDNA and describe its spatio-temporal mRNA expression within the developing larval brain and visual system. Expression of the 5-HT2B transcripts is compared to that of 5-HT2C as well as proliferation and neurogenic markers. In developing brain and retina, 5-HT2B and 2C mRNAs are mainly expressed in proliferative regions. We suggest that these receptors may play a role in the larval secondary neurogenesis by mediating mitogenic effects of serotonin.
authors.affiliation: Laboratori di Biologia Cellulare e dello Sviluppo, Dipartimento di Fisiologia e Biochimica, Università di Pisa, Via Carducci 13, 56010 Ghezzano, Pisa, Italy. sdelu@dfb.unipi.it
authors.forename: Stefania
authors.initials: S
authors.lastname: De Lucchini
authors.forename: Michela
authors.initials: M
authors.lastname: Ori
authors.forename: Martina
authors.initials: M
authors.lastname: Nardini
authors.forename: Silvia
authors.initials: S
authors.lastname: Marracci
authors.forename: Irma
authors.initials: I
authors.lastname: Nardi
mesh_terms: D000818:Animals; D001921:Brain / Q000196:embryology / Q000378:metabolism*; D001973:Bromodeoxyuridine / Q000493:pharmacokinetics; D003001:Cloning, Molecular; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D004625:Embryo, Nonmammalian; D005123:Eye / Q000196:embryology / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D005819:Genetic Markers; D017403:In Situ Hybridization; D007814:Larva / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis; D011838:Radiation-Sensitizing Agents / Q000493:pharmacokinetics; D051881:Receptor, Notch1; D044402:Receptor, Serotonin, 5-HT2A; D044403:Receptor, Serotonin, 5-HT2B; D011956:Receptors, Cell Surface; D011985:Receptors, Serotonin / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014157:Transcription Factors; D014981:Xenopus / Q000235:genetics; D029867:Xenopus Proteins / Q000378:metabolism
chemical_list: D005819:Genetic Markers; D008565:Membrane Proteins; D012333:RNA, Messenger; D011838:Radiation-Sensitizing Agents; D051881:Receptor, Notch1; D044402:Receptor, Serotonin, 5-HT2A; D044403:Receptor, Serotonin, 5-HT2B; D011956:Receptors, Cell Surface; D011985:Receptors, Serotonin; D014157:Transcription Factors; D029867:Xenopus Proteins; D019938:Cyclin D1; D001973:Bromodeoxyuridine