Characterization of Pellino2, a substrate of IRAK1 and IRAK4.
Interleukin-1 (IL-1) receptor-associated kinases (IRAKs) are central components of Toll/IL-1 receptor (TIR) signaling pathways. In an attempt to discover novel signal transducers in TIR signaling, we identified human Pellino2 as an interaction partner of IRAK4. Pellino2 interacts with kinase-active as well as kinase-inactive IRAK1 and IRAK4. Furthermore, Pellino2 is one of the first substrates identified for IRAK1 and IRAK4. Functional studies using overexpression or RNAi knock-down of Pellino2 suggest a role of Pellino2 as a scaffolding protein similar to Pellino1. However, unlike Pellino1, Pellino2 does not seem to activate a specific transcription factor, but links TIR signaling to basic cellular processes.
Strelow
Astrid
A
Tularik Inc., 1120 Veterans Boulevard, South San Francisco, CA 94080, USA.
Kollewe
Christian
C
Wesche
Holger
H
D002460:Cell Line; D002478:Cells, Cultured; D003001:Cloning, Molecular; D017930:Genes, Reporter; D005822:Genetic Vectors; D006801:Humans; D053592:Interleukin-1 Receptor-Associated Kinases; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism*; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity; D044767:Ubiquitin-Protein Ligases
D009687:Nuclear Proteins; D011994:Recombinant Proteins; C485959:PELI1 protein, human; D044767:Ubiquitin-Protein Ligases; D011494:Protein Kinases; D017853:Phosphotransferases (Alcohol Group Acceptor); C507768:IRAK1 protein, human; C507773:IRAK4 protein, human; D053592:Interleukin-1 Receptor-Associated Kinases