Simple and rapid methods for SEM observation and TEM immunolabeling of rubber particles.
We developed a method involving air-drying of a rubber suspension after fixation in glutaraldehyde-tannic acid and postfixation in osmium tetroxide for SEM observation. For TEM immunolabeling the suspension was air-dried after osmium-only fixation. Whereas conventional methods failed to satisfactorily stabilize rubber particles, the methods described here proved successful in preserving their integrity.
Singh
Adya P
AP
Agricultural Plant Stress Research Center, Chonnam National University, Gwangju, Republic of Korea. adyasingh@hotmail.com
Wi
Seung Gon
SG
Kang
Hunseung
H
Chung
Gap Chae
GC
Kim
Yoon Soo
YS
D030681:Ficus / Q000737:chemistry; D005404:Fixatives; D028482:Hevea / Q000737:chemistry; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D012408:Rubber / Q000737:chemistry*
D005404:Fixatives; D012408:Rubber