title: A novel potentiometric approach for detection of beta-adrenergics and beta-adrenolytics in high-performance liquid chromatography.
abstract: Potentiometric approach enabling sensitive and reliable detection for a series of 20 autonomic beta-adrenergic ligands with the use of poly(vinyl chloride) (PVC) based liquid membrane electrode coatings in the normal-bore cation exchange HPLC and narrow-bore reversed phase HPLC system is presented. It was found that in both kinds of HPLC modes with a contemporary hybrid polymer-silica packings an application of electrodes containing a tetrakis(p-chlorophenyl)borate (TCPB) gives limits of detection below to 8.0x10(-7) mol l(-1) (injected concentrations). In case of highly hydrophobic beta-adrenergic drugs the use of binary aqueous mobile phases with high concentrations of acetonitrile (up to 25% v/v) shifting an observable detection limits (DL) down to 2.0x10(-8) mol l(-1), especially for electrodes with addition of trioctylated alpha-cyclodextrin. The characteristics of developed potentiometric detectors was established by proposed a quantitative structure-potentiometric response relationships (QSPRRs) for a series of diversified beta-adrenergic compounds and for a set of the PVC based electrodes using TCPB alone as well as in combination with trioctylated alpha-cyclodextrin, dibenzo-18-crown-6, or calix[6]arene hexaethylester as the neutral macrocycle ionophore. A highly significant QSPRRs equations were obtained leading to reasonable prediction of the DL of specified electrodes in terms of the computationally derived set of molecular descriptors of beta-adrenergics and beta-blocking agents and similar amino alcohol type xenobiotics.
authors.affiliation: Department of Drug Chemistry, Faculty of Pharmacy, Ludwik Rydygier Medical University, Jagiellonska 13, PL-85-067 Bydgoszcz, Poland. gbazylak@amb.bydgoszcz.pl
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Bazylak
authors.forename: Luc J
authors.initials: LJ
authors.lastname: Nagels
mesh_terms: D018674:Adrenergic Antagonists / Q000032:analysis* / Q000737:chemistry; D000318:Adrenergic beta-Agonists / Q000032:analysis* / Q000737:chemistry; D000319:Adrenergic beta-Antagonists / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods; D003505:Cyclodextrins / Q000737:chemistry; D004566:Electrodes; D004988:Ethers, Cyclic / Q000737:chemistry; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008567:Membranes, Artificial; D008958:Models, Molecular; D010636:Phenols / Q000737:chemistry; D011143:Polyvinyl Chloride / Q000737:chemistry; D011199:Potentiometry / Q000379:methods*; D021281:Quantitative Structure-Activity Relationship; D012463:Saliva / Q000737:chemistry; D012680:Sensitivity and Specificity; D013775:Tetraphenylborate / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D018674:Adrenergic Antagonists; D000318:Adrenergic beta-Agonists; D000319:Adrenergic beta-Antagonists; D003505:Cyclodextrins; D004988:Ethers, Cyclic; D008567:Membranes, Artificial; D010636:Phenols; C030385:tetrakis(4-chlorophenyl)borate; D013775:Tetraphenylborate; D011143:Polyvinyl Chloride