Pex10p links the ubiquitin conjugating enzyme Pex4p to the protein import machinery of the peroxisome.
The protein import machinery of the peroxisome consists of many proteins, collectively called the peroxins. By applying the split-ubiquitin technique we systematically tested the pair-wise interactions between the Nub- and Cub-labeled peroxins for the first time in the living cells of the yeast Saccharomyces cerevisiae. We found that Pex10p plays a central role in the protein interaction network by connecting the ubiquitin conjugation enzyme Pex4p to the other members of the protein import machinery. A yeast strain harboring a deletion of PEX3 enabled us to estimate the influence of the peroxisomal membrane on the formation of a subset of the investigated protein-protein interactions.
Eckert
Jörg H
JH
Ruhr-Universität Bochum, Institut für Physiologische Chemie, Medizinische Fakultät, 44780 Bochum, Germany.
Johnsson
Nils
N
D001692:Biological Transport; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D008958:Models, Molecular; D000074401:Peroxins; D000074428:Peroxisomal Biogenesis Factor 2; D020675:Peroxisomes / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases
D005656:Fungal Proteins; D008565:Membrane Proteins; C492057:PEX10 protein, S cerevisiae; C491335:PEX12 protein, S cerevisiae; C070895:PEX3 protein, S cerevisiae; C076587:PEX4 protein, S cerevisiae; D000074401:Peroxins; D018160:Receptors, Cytoplasmic and Nuclear; D029701:Saccharomyces cerevisiae Proteins; D000074428:Peroxisomal Biogenesis Factor 2; D044767:Ubiquitin-Protein Ligases