Pancreatic NOD beta cells express MHC class II protein and the frequency of I-A(g7) mRNA-expressing beta cells strongly increases during progression to autoimmune diabetes.
OBJECTIVE
In the NOD mouse model, attempts to show MHC class II expression by pancreatic beta cells were unsuccessful so far. We readdressed this question by analysing I-A(g7) expression in single pancreatic beta cells.


METHODS
Single-cell multiplex RT PCR and single-cell immunofluorescence were used to study MHC class II expression in NOD and NOD/SCID beta cells.


RESULTS
Pancreatic beta cells from NOD mice express the I-A(g7) protein as well as the corresponding mRNA. The frequency of MHC class II mRNA-expressing beta cells is drastically increased during the progression to overt diabetes. MHC class II protein is accumulated intracellularly, and invariant chain is co-expressed. Beta cells from 9- to 10-week-old NOD/SCID mice express MHC class II at the same low frequency as beta cells from 3-week-old NOD mice.


CONCLUSIONS
NOD beta cells express I-A(g7) and could be a direct target of autoreactive CD4+ T cells. This MHC class II expression is triggered by infiltrating lymphocytes.
Walter
U
U
Department of Cellbiology and Immunology, German Research Centre for Biotechnology, Braunschweig, Germany. Ulrich.Walter@med.uni-duesseldorf.de
Toepfer
T
T
Dittmar
K E J
KE
Kretschmer
K
K
Lauber
J
J
Weiss
S
S
Servos
G
G
Lechner
O
O
Scherbaum
W A
WA
Bornstein
S R
SR
Von Boehmer
H
H
Buer
J
J
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics* / Q000276:immunology*; D018450:Disease Progression; D005786:Gene Expression Regulation / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D007515:Islets of Langerhans / Q000276:immunology*; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D012333:RNA, Messenger / Q000235:genetics; D013997:Time Factors; D014158:Transcription, Genetic / Q000276:immunology*
D000949:Histocompatibility Antigens Class II; C407099:I-A g7 antigen; D012333:RNA, Messenger