title: Antimicrobial activity and interference of tobramycin and chloramphenicol on bacterial adhesion to intraocular lenses.
abstract: The antimicrobial activities of tobramycin and chloramphenicol were evaluated by determining minimum inhibitory and bactericidal concentrations against Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, group A, group B and group G streptococci, Klebsiella spp., Stenotrophomonas maltophilia and ciprofloxacin-resistant and -susceptible Pseudomonas aeruginosa, as well as by evaluating interference on adhesion of slime producer strains of S. aureus and P. aeruginosa to intraocular lens from tobramycin and chloramphenicol pharmaceutical products by scanning electron microscopy. Chloramphenicol was more active against Gram-positive bacteria than was tobramycin, which instead showed higher activity against ciprofloxacin-susceptible P. aeruginosa. Treatment of lenses with the antimicrobial products eradicated the bacterial biofilm, which was already notably reduced after 5 min. This activity was more pronounced for chloramphenicol against S. aureus and for tobramycin against P. aeruginosa. Bacterial adhesion was also significantly reduced when lenses colonized by P. aeruginosa were treated with chloramphenicol, even if they were resistant to this drug. In conclusion, the tested drugs showed marked antibacterial activity, particularly by interfering with bacterial biofilms. The data obtained in this study suggest a specific use of chloramphenicol in topical prophylaxis aimed at avoiding bacterial contaminations. However, further specific in vivo studies are needed to confirm these data.
authors.affiliation: Microbiology Laboratory, Department of Clinical Sciences, L. Sacco Teaching Hospital, University of Milan, Milan, Italy. microbio@mailserver.unimi.it
authors.forename: L
authors.initials: L
authors.lastname: Drago
authors.forename: E
authors.initials: E
authors.lastname: De Vecchi
authors.forename: L
authors.initials: L
authors.lastname: Nicola
authors.forename: M R
authors.initials: MR
authors.lastname: Gismondo
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects*; D018441:Biofilms; D002701:Chloramphenicol / Q000494:pharmacology*; D005782:Gels; D007910:Lenses, Intraocular / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D011208:Powders; D014031:Tobramycin / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D005782:Gels; D011208:Powders; D002701:Chloramphenicol; D014031:Tobramycin