title: Metabolism of bisphenol A in zebrafish (Danio rerio) and rainbow trout (Oncorhynchus mykiss) in relation to estrogenic response.
abstract: The kinetics of bisphenol A (BPA) were investigated in zebrafish (Danio rerio) exposed to 100 microg BPA/l. BPA uptake was measured during a 7-day period followed by an elimination phase of similar duration. After 2, 6, 12, 24, 48, 72, 120 and 168 h of uptake/elimination, fish were analysed for their content of BPA, bisphenol A glucuronic acid (BPAGA) and bisphenol A sulfate (BPAS). Within the first 24 h steady state levels of BPA, BPAGA and BPAS were reached and the total body concentrations were calculated to be 569, 12,600 and 39.9 ng/g fish, respectively. Elimination rates of the three compounds in zebrafish were estimated by fitting the data to a compartment model. An initial rapid elimination phase was observed for BPA and BPAS with total body half lives (T(1/2)) of <1.1 h and 30 min, followed by a slower second elimination phase with T(1/2) values of 139 and 71 h, respectively. Excretion of BPAGA occurred from a single compartment with a T(1/2) of 35 h. The steady state concentration of BPA and its metabolites were investigated in rainbow trout (Oncorhynchus mykiss) exposed to 100 microg BPA/l. The toxicokinetic parameters from zebrafish and rainbow trout were compared; including previously published data on the rainbow trout. The data indicate that the smaller estrogenic sensitivity observed for the zebrafish may be caused by a more rapid metabolism of BPA in the zebrafish liver.
authors.affiliation: Institute of Biology, University of Southern Denmark, Campusvej 55, Odense M DK-5230, Denmark.
authors.forename: C
authors.initials: C
authors.lastname: Lindholst
authors.forename: P M
authors.initials: PM
authors.lastname: Wynne
authors.forename: P
authors.initials: P
authors.lastname: Marriott
authors.forename: S N
authors.initials: SN
authors.lastname: Pedersen
authors.forename: P
authors.initials: P
authors.lastname: Bjerregaard
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds; D001646:Bile / Q000378:metabolism; D004967:Estrogens / Q000378:metabolism*; D005260:Female; D006207:Half-Life; D008099:Liver / Q000378:metabolism; D008297:Male; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D010636:Phenols / Q000097:blood / Q000378:metabolism / Q000493:pharmacokinetics* / Q000633:toxicity; D015027:Zebrafish / Q000378:metabolism*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004967:Estrogens; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds