Generation of canine dendritic cells from peripheral blood mononuclear cells.
Dendritic cells (DCs) are the most potent antigen-presenting cells that are expected to be therapeutic agents for tumor immunotherapy. In this study, we generated DCs of sufficient number for DC-based immunotherapy from peripheral blood mononuclear cells (PBMC) in dogs. PBMC were cultured in the presence of phytohemagglutinin (PHA). On day 6, large adherent cells with dendrite-like projections were seen, and the number of these large cells with projections increased on day 8. These cells were positive for esterase staining. They expressed MHC class II, CD11b, CD8 and weakly CD4 on their surface. They tended to make contact with lymphocytes under culture conditions. We obtained about 2-5 x 10(6) of DCs from 10 ml of peripheral blood. These DCs phagocytosed HEK-293 cells by overnight co-culturing. These cells generated from PBMC are possible canine DCs and are applicable to clinical trials of DC-based whole tumor cell immunotherapy in dogs.
Yoshida
Hiroko
H
Department of Veterinary Surgery, Faculty of Agriculture, Gifu University, Gifu, Japan.
Momoi
Yasuyuki
Y
Taga
Natsuko
N
Ide
Kaori
K
Yamazoe
Kazuaki
K
Iwasaki
Toshiroh
T
Kudo
Tadaaki
T
D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D018920:Coculture Techniques; D006224:Cricetinae; D003470:Culture Media / Q000737:chemistry; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D004285:Dogs; D004950:Esterases / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000187:drug effects; D010587:Phagocytosis; D010835:Phytohemagglutinins / Q000494:pharmacology
D015703:Antigens, CD; D003470:Culture Media; D010835:Phytohemagglutinins; D004950:Esterases