Formation of phenylethanoid glycosides by Cistanche deserticola callus grown on solid media.
The optimal growth of Cistanche deserticola callus and formation of phenylethanoid glycosides (PeG) was at 25 degrees C with light irradiation intensity of 24 micromol m(-2) s(-1) on solidified B5 media supplemented with 0.5 mg 6-benzylaminopurine l(-1), 10 mg gibberellin l(-1), 800 mg casein hydrolysate l(-1) and 20 g sucrose l(-1). After 30 d culture, the biomass reached 15.5 g dry wt callus l(-1) medium and its PEG content was 10.7% (w/w). The PeG content was 42%-127% higher than those in explants.
Ouyang
Jie
J
State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, PO Box 353, Beijing 100080, China. joyouyang@yahoo.com
Wang
Xiao-dong
XD
Zhao
Bing
B
Wang
Yu-chun
YC
D002364:Caseins / Q000378:metabolism; D031671:Cistanche / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism* / Q000528:radiation effects; D003470:Culture Media / Q000494:pharmacology; D046508:Culture Techniques / Q000379:methods*; D006027:Glycosides / Q000096:biosynthesis*; D008027:Light; D010937:Plant Growth Regulators / Q000494:pharmacology; D011492:Protein Hydrolysates / Q000378:metabolism; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013395:Sucrose / Q000378:metabolism
D002364:Caseins; D003470:Culture Media; D006027:Glycosides; D010937:Plant Growth Regulators; D011492:Protein Hydrolysates; C026375:trypticase-soy broth; D013395:Sucrose