title: Protective effect of polypeptide from Chlamys farreri on mitochondria in human dermal fibroblasts irradiated by ultraviolet B.
abstract: OBJECTIVE
  To study the effect of polypeptide from Chlamys farreri (PCF) on mitochondria of human dermal fibroblasts irradiated by ultraviolet B (UVB) in vitro.
  
  
  METHODS
  Malondialdehyde (MDA) and antioxidant enzymes including superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) were determined by biochemical methods. Mitochondrial transmembrane potential was measured by flow cytometry. Ultrastructure of fibroblasts was observed with transmission electron microscope.
  
  
  RESULTS
  UVB (1.176 x 10(-4) J/cm(2)) induced mitochondria damage in dermal fibroblast and PCF (0.25%-1%) reduced the damage in a concentration-dependent manner. Furthermore, PCF also concentration-dependently maintained the stability of mitochondrial transmembrane potential. PCF was able to reduce the MDA formation caused by UVB, meanwhile increased the activities of SOD and GSH-PX. The differences among the PCF groups and UVB model group were significant (P<0.05, P<0.01).
  
  
  CONCLUSIONS
  The UVB-induced mitochondria damage was alleviated by PCF in human dermal fibroblasts.
authors.affiliation: Department of Pharmacology, Medical College, Qingdao University, Qingdao 266021, China. liujg666@yahoo.com.cn
authors.forename: Chun-Bo
authors.initials: CB
authors.lastname: Wang
authors.forename: Bo-Xiao
authors.initials: BX
authors.lastname: Ding
authors.forename: Shen-Bo
authors.initials: SB
authors.lastname: Guo
authors.forename: Yu-Zhen
authors.initials: YZ
authors.lastname: Wang
authors.forename: Yan-Tao
authors.initials: YT
authors.lastname: Han
authors.forename: Yue-Jun
authors.initials: YJ
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002478:Cells, Cultured; D020405:Dermis / Q000166:cytology; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects* / Q000528:radiation effects / Q000648:ultrastructure; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D008315:Malondialdehyde / Q000378:metabolism; D008419:Materia Medica / Q000302:isolation & purification / Q000494:pharmacology*; D008564:Membrane Potentials; D008928:Mitochondria / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects; D008974:Mollusca / Q000737:chemistry*; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D000975:Antioxidants; D008419:Materia Medica; D010455:Peptides; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase