[Regulation of the neuronal functional state by ultra low doses of different biologically active substances. Nonspecific effect ].
The role of biologically active substances in ultra-low doses (10(-15)-10(-27) mol/l) is discussed from the different points of view. The most detailed analysis of neurobiological effects produced by these doses can be studied on the preparate of completely isolated molluscan neurones. In this case the possibility arises to control the first modifications of action at the electrophysiological characteristics of neuronal activity. These changes of electrical activity can be regarded as a reaction to biologically active substance. The following characteristics were controlled: the level of membrane resting potential (MP), the electroexcitable membrane and pacemaker mechanism, chemical sensitivity of somatic membrane loci to neurotransmitter acetylcholine (Ach). Several substances were used in these experiments: two kinds of synthetic antioxidant, GABA, ethanol, serotonine, DSIP (delta-sleep inducing peptide), antibiotic ruboxil, nootrop GVS-111. The isolated neurones were placed into the special chamber. All these substances (0.35 ml) were added single dosing into this chamber with living physiological solution in concentration 10(-15)-10(-27) mol/l. The results demonstrated that all substances had initiated the development of prolonged neurophysiological responses. The intensities of neuronal reactions didn't depend in contact period on the concentration and on the type of substance. It is suggested that these data reveal the existence of unknown modes of regulation of neuronal functional states and presence of hidden channel for information transfer and receiving. This different way of regulation is extremely important influence living organisms.
Terekhova
S F
SF
Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, 119991 Russia. seren@sky.chph.ras.ru
Grechenko
T N
TN
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects; D003701:Delta Sleep-Inducing Peptide / Q000494:pharmacology; D004151:Dipeptides / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004594:Electrophysiology / Q000379:methods; D000431:Ethanol / Q000494:pharmacology; D006372:Helix, Snails; D066298:In Vitro Techniques; D016014:Linear Models; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000187:drug effects* / Q000502:physiology*; D018377:Neurotransmitter Agents / Q000494:pharmacology*; D012701:Serotonin / Q000494:pharmacology; D013997:Time Factors; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D000975:Antioxidants; D004151:Dipeptides; D018377:Neurotransmitter Agents; D012701:Serotonin; D000431:Ethanol; C103871:ethyl phenylacetyl-Pro-Gly; D005680:gamma-Aminobutyric Acid; D003701:Delta Sleep-Inducing Peptide; D000109:Acetylcholine