title: Interleukin-6 mediates lipopolysaccharide-induced nuclear STAT3 translocation in astrocytes of rat sensory circumventricular organs.
abstract: Pyrogenic treatment with lipopolysaccharide (LPS) or interleukin-6 (IL-6) induces nuclear STAT3 translocation in the vascular organ of the laminae terminalis (OVLT) and the subfornical organ (SFO). STAT3 immunohistochemistry was combined with the detection of marker proteins (glial, neuronal, endothelial) and the nuclear DAPI stain to determine the phenotype of responding cells. At time points with high pyrogen-induced IL-6 plasma levels, nuclear STAT3 signals were co-localized with an astrocytic cell marker. IL-6 might therefore mediate genomic activation of OVLT/SFO astrocytes during LPS-induced fever.
authors.affiliation: Institute of Veterinary-Physiology, Justus-Liebig-University Giessen, Frankfurter Strasse 100, D-35392 Giessen, Germany.
authors.forename: Eva Maria
authors.initials: EM
authors.lastname: Harré
authors.forename: Joachim
authors.initials: J
authors.lastname: Roth
authors.forename: Rüdiger
authors.initials: R
authors.lastname: Gerstberger
authors.forename: Thomas
authors.initials: T
authors.lastname: Hübschle
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005334:Fever / Q000378:metabolism; D007031:Hypothalamus / Q000378:metabolism*; D007150:Immunohistochemistry; D015850:Interleukin-6 / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D050796:STAT3 Transcription Factor; D013356:Subfornical Organ / Q000378:metabolism*; D015534:Trans-Activators / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D015850:Interleukin-6; D008070:Lipopolysaccharides; D050796:STAT3 Transcription Factor; C494088:Stat3 protein, rat; D015534:Trans-Activators