Continuous cultures of Clostridium acetobutylicum: culture stability and low-grade glycerol utilisation.
Continuous cultures of two strains of Clostridium acetobutylicum were stable for over 70 d when grown on glucose/glycerol mixtures. Butanol was the major fermentation end-product, accounting for 43 to 62% (w/w) of total products. Low-grade glycerol [65% (w/v) purity] could replace commercial glycerol [87% (w/v) purity], leading to a similar fermentation pattern: a butanol yield of 0.34 (mol/mol), a butanol productivity of 0.42 g l(-1) h(-1) and a 84% (w/w) glycerol consumption were attained when cultures were grown at pH 6 and D = 0.05 h(-1); butanol accounted for 94% (w/w) of total solvents. These values are among the highest reported in literature for C. acetobutylicum simple chemostats.
Andrade
José Carlos
JC
Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Porto, Portugal.
Vasconcelos
Isabel
I
D019149:Bioreactors; D000440:Butanols / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods; D003013:Clostridium / Q000145:classification / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D005990:Glycerol / Q000145:classification / Q000378:metabolism*; D011786:Quality Control
D000440:Butanols; D005947:Glucose; D005990:Glycerol