Biomedical applications of capillary electrophoresis.
Capillary electrophoresis (CE) is a technique well suited for several separation problems in the life sciences. The main advantages are the higher separation efficacy in comparison to chromatographic methods and the smaller sample volume required. However, due to the limited sensitivity of CE, HPLC remains the method most commonly used for the analysis of drugs in biological fluids. For endogenous compounds like DNA, proteins, or small molecules like purines, CE offers clear advantages over conventional methods and, especially for DNA, CE has already been introduced into clinical routine. Some selected applications will be discussed.
Hempel
Georg
G
Institute for Pharmaceutical and Medical Chemistry, Muenster, Germany. hempege@uni-muenster.de
D000596:Amino Acids / Q000032:analysis; D035843:Biomedical Research / Q000379:methods*; D001798:Blood Proteins / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D011687:Purines / Q000652:urine; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000596:Amino Acids; D001798:Blood Proteins; D011687:Purines; D004247:DNA