title: Observation and analysis of single DNA nano-kinetics by pin-fiber video scope.
abstract: The nano-kinetic movement of a single DNA molecule was observed and analyzed by a newly developed video-microscope system with an optical fiber, called a pin-fiber video scope. A single lambda-DNA molecule was put in focus using fiber-illumination, and the stretching and shrinking motion was measured. The molecule's kinetics were analyzed by numerical calculations and are discussed. A photocleavage phenomenon of DNA molecules was also visualized by the pin-fiber video scope. The new video-microscope system has the potential to observe and analyze the nano-kinetics of a single molecule.
authors.affiliation: Graduate School of Medical Science, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan. yhirakaw@hiroshima-u.ac.jp
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Hirakawa
authors.forename: Masashi
authors.initials: M
authors.lastname: Suzuto
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ohnishi
authors.forename: Tomoteru
authors.initials: T
authors.lastname: Shingaki
authors.forename: Edward M
authors.initials: EM
authors.lastname: Eyring
authors.forename: Makio
authors.initials: M
authors.lastname: Tokunaga
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Masujima
mesh_terms: D004247:DNA / Q000378:metabolism*; D005336:Fiber Optic Technology; D006801:Humans; D018715:Microscopy, Video; D036103:Nanotechnology / Q000379:methods*; D055100:Optical Fibers
chemical_list: D004247:DNA