title: Genotypic analysis in primary systemic anaplastic large cell lymphoma.
abstract: This report presents an experience of polymerase chain reaction (PCR) analysis of T-cell receptor g- and bgene (TCR g, TCR b), and immunoglobulin heavy chain (IgH) gene rearrangements in 9 cases of primary systemic anaplastic large cell lymphoma. We showed 2 clonal IgH, 2 TCR g, 1 TCR b rearrangements. The genotype was B/T-cell in 1, T-cell in 1, B-cell in 1 and null cell-type in 6 cases. We used reverse transcriptase PCR (RT-PCR) to detect t(2;5)(p23;q35) and t(1;2)(q25;p23) translocations. T(2;5) translocation was demonstrated in 2 cases, there was no t(1;2) translocation. In most cases the molecular genetic results were found to be in agreement with immunophenotypic data.
authors.affiliation: First Department of Medicine, University of Pécs, 7624 Pécs, Hungary. aszomor@clinics.pote.hu
authors.forename: Arpád
authors.initials: A
authors.lastname: Szomor
authors.forename: Prune
authors.initials: P
authors.lastname: Zenou
authors.forename: Daniel
authors.initials: D
authors.lastname: Roda
authors.forename: Talal
authors.initials: T
authors.lastname: Al Saati
authors.forename: György
authors.initials: G
authors.lastname: Csanaky
authors.forename: László
authors.initials: L
authors.lastname: Pajor
authors.forename: Gábor
authors.initials: G
authors.lastname: Kelényi
authors.forename: Georges
authors.initials: G
authors.lastname: Delsol
authors.forename: Hajna
authors.initials: H
authors.lastname: Losonczy
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics; D002895:Chromosomes, Human, Pair 5 / Q000235:genetics; D005260:Female; D015321:Gene Rearrangement / Q000235:genetics*; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor / Q000235:genetics*; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor / Q000235:genetics*; D005803:Genes, Immunoglobulin / Q000235:genetics*; D005838:Genotype; D006801:Humans; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014178:Translocation, Genetic