[The effect of SP-8-CPT-cAMP on the tracer coupling between the retinal ganglion cells and amacrine cells].
OBJECTIVE
To study the effect of SP-8-CPT-cAMP on the tracer coupling between the retinal ganglion cells and amacrine cells in rabbits.


METHODS
The retinas of rabbit were cultured and treated with SP-8-CPT-cAMP .4% neurobiotin was injected into retinal single OFF-alpha ganglion cell with micro-injection, following tissue fixation with 4% paraformaldehyde, Cells were visualized with 1:600 streptavidin-Cy3. The intensity of tracer diffusing through the gap junctions among the ganglion cells and amacrine cells was calculated with confocal microscope.


RESULTS
In the dendritic field of injected ganglion cells and cross the field some other ganglion cells(G1) and amacrine cells locating in the inner nuclear layer were stained. Two types of amacrine cells: brighter one (A1) and dimmer one (A2) were found. The intensity of neurobiotin in coupled G1,A1,A2 after treated with SP-8-CPT-cAMP is much higher than that of the untreated retinas.


CONCLUSIONS
The gap junctions exist among the OFF-alpha ganglion cells and between OFF-alpha ganglion cells and amacrine cells. SP-8-CPT-cAMP increases the permeability of gap junction in OFF-alpha ganglion cell network.
Xia
Xiaobo
X
Department of Ophthalmology, Xiangya Hospital, Central South University, Changsha 410008.
Zhou
Xia
X
Mills
Stephen L
SL
D025042:Amacrine Cells / Q000166:cytology*; D000818:Animals; D001710:Biotin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000031:analogs & derivatives* / Q000494:pharmacology*; D003712:Dendrites; D005260:Female; D017629:Gap Junctions; D008297:Male; D018613:Microscopy, Confocal; D009474:Neurons / Q000166:cytology; D011817:Rabbits; D011897:Random Allocation; D012165:Retinal Ganglion Cells / Q000166:cytology*; D013873:Thionucleotides / Q000494:pharmacology*
D013873:Thionucleotides; C070614:neurobiotin; C015929:8-((4-chlorophenyl)thio)cyclic-3',5'-AMP; D001710:Biotin; D000242:Cyclic AMP