title: Identification of androgen-regulated genes in SV40-transformed human hair dermal papilla cells.
abstract: BACKGROUND
  Androgens are major regulators of human hair growth, which exert their effect on follicular epithelium via the mesenchyme-derived dermal papilla (DP) cells. However, very few data are available with regard to the genes regulated by androgen in DP cells.
  
  
  OBJECTIVE
  To investigate the differentially expressed genes by androgen in DP cells.
  
  
  METHODS
  Human hair DP cells were cultured and transformed with SV40 T antigen. Using cDNA representational difference analysis, androgen-regulated genes in SV40-transformed dermal papilla (SDP) cells were identified.
  
  
  RESULTS
  SDP cells revealed extended lifespan as compared with primary cultured DP cells. SDP cells expressed androgen receptor (AR) and showed androgen responsiveness. cDNA representational difference analysis followed by Northern blot analysis showed that heat shock cognate protein, Hsc70 was differentially expressed by dihydrotestosterone (DHT) treatment in SV40-transformed DP cells.
  
  
  CONCLUSIONS
  These results suggest that Hsc70 may be involved in androgen action on DP cells.
authors.affiliation: Oriental Hospital, Oriental Medical College of Daejeon University, 22-5 Daeheung-dong, 301-724, Daejeon, South Korea.
authors.forename: Chang Deok
authors.initials: CD
authors.lastname: Kim
authors.forename: Min Ho
authors.initials: MH
authors.lastname: Lee
authors.forename: Seok Seon
authors.initials: SS
authors.lastname: Roh
mesh_terms: D000728:Androgens / Q000494:pharmacology / Q000502:physiology*; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D013196:Dihydrotestosterone / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D018859:Hair Follicle / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D011944:Receptors, Androgen / Q000378:metabolism; D013539:Simian virus 40
chemical_list: D000728:Androgens; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; C494371:HSPA8 protein, human; D011944:Receptors, Androgen; D013196:Dihydrotestosterone