title: Effects of adrenalectomy on CART expression in the rat arcuate nucleus.
abstract: In order to test for glucocorticoid regulation of CART in the arcuate nucleus, adrenalectomies (ADX) and hormone replacements (HRs) were carried out in groups of rats. CART mRNA levels were determined by in situ hybridization and CART peptide levels by immunocytochemistry. ADX caused a lowering of CART mRNA and peptides levels in the arcuate and this was reversed by HR. These results indicate a glucocorticoid regulation of CART in the arcuate. The regulation could be direct through an action of glucocorticoid receptors or indirectly through ADX-induced changes in leptin levels. These findings suggest a role for CART in the stress response.
authors.affiliation: Ege University Center for Brain Research, School of Medicine, Department of Physiology, Bornova, Izmir, Turkey.
authors.forename: Burcu
authors.initials: B
authors.lastname: Balkan
authors.forename: Ersin
authors.initials: E
authors.lastname: Koylu
authors.forename: Sakire
authors.initials: S
authors.lastname: Pogun
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Kuhar
mesh_terms: D000302:Adrenal Cortex / Q000293:injuries / Q000378:metabolism*; D000315:Adrenalectomy; D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000378:metabolism*; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D005938:Glucocorticoids / Q000172:deficiency* / Q000494:pharmacology; D007150:Immunohistochemistry; D009419:Nerve Tissue Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000187:drug effects / Q000378:metabolism; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein
chemical_list: D005938:Glucocorticoids; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D011965:Receptors, Glucocorticoid; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein