Rapid preparation of DNA microarray using PCR for hepatitis B and D virus detection.
OBJECTIVE
To prepare DNA microarray for detecting both hepatitis B and D virus as (HBV and HDV).


METHODS
With the assistance of Oligo6.4 software, specific PCR primers targeting the conserved region of HBV and HDV were designed. The PCR products were purified and cloned into the pMD18-T vectors, followed by rapid identification. The recombinant plasmids were then extracted from positive clones and the target gene fragments underwent sequence analysis.


RESULTS
The gene fragments of HBV and HDV were obtained by PCR, which were confirmed by sequence analysis to be specific gene fragments of HBV and HDV.


CONCLUSIONS
Using PCR amplification products to prepare the DNA microarray is quick, simple and effective.
Sun
Zhao-hui
ZH
Institute of Molecular Oncology, Guangzhou General Hospital of Guangzhou Command, Guangzhou 510010, China.
Zheng
Wen-ling
WL
Mao
Xiang-ming
XM
Zhang
Bao
B
Lü
Liang
L
Ma
Xiao-dong
XD
Shi
Rong
R
Ma
Wen-li
WL
D006515:Hepatitis B virus / Q000235:genetics / Q000302:isolation & purification*; D003698:Hepatitis Delta Virus / Q000235:genetics / Q000302:isolation & purification*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*