HPLC studio: a novel software utility to perform HPLC chromatogram comparison for screening purposes.
A new tool, HPLC Studio, was developed for the comparison of high-performance liquid chromatography (HPLC) chromatograms from microbial extracts. The new utility makes it possible to create a virtual chromatogram by mixing up to 20 individual chromatograms. The virtual chromatogram is the first step in establishing a ranking of the microbial fermentation conditions based on either the area or diversity of HPLC peaks. The utility was used to maximize the diversity of secondary metabolites tested from a microorganism and therefore increase the chances of finding new lead compounds in a drug discovery program.
García
J B
JB
Centro de Investigación Básica (CIBE), Merck, Sharp & Dohme España, Madrid, Spain. juan_bautista_garcia@merck.com
Tormo
José R
JR
D000465:Algorithms; D019149:Bioreactors; D002845:Chromatography / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005285:Fermentation; D015723:Gene Library; D000432:Methanol / Q000494:pharmacology; D012680:Sensitivity and Specificity; D012984:Software
D000432:Methanol