[Polymorphism of CXCR4 coding region of human immunodeficiency virus-1 in Chinese Han people].
OBJECTIVE
To study the polymorphism of human immunodeficiency virus (HIV)-1 coreceptor CXCR4 in Chinese Han ethnic group for AIDS prevention and treatment.


METHODS
Totally 48 individuals were enrolled into the study. CXCR4 (cDNA No-AF147204) was cloned by PCR amplification using 2 pairs of primers, then sequenced using sequencing primers. The results of the same sequencing primers were analyzed by DNAstar software to find and identify single nucleotide polymorphism (SNP) sites.


RESULTS
Totally 7 SNPs were found in the coding region of CXCR4, among them 3 were synonymous mutation (C-->T at loci 129, 426 and 968), 3 were missense mutation (C-->T at locus 38, A-->T at locus 90, and A-->C at locus 712) and 1 was stop mutation (C-->T at 106, which converted the codon for glutamic acid into stop codon).


CONCLUSIONS
The polymorphism of CXCR4 coding region in Chinese Han is probably different from that of the other ethnic groups. Six of the 7 SNPs were discovered for the first time. Their influences on AIDS progression are worthy of studying.
Liu
Ming-xu
MX
The No. 302 Hospital of PLA, Beijing 100039, China.
Wang
Fu-sheng
FS
Hong
Wei-guo
WG
Wang
Bo
B
Jin
Lei
L
Lei
Zhou-yun
ZY
Hou
Jing
J
D000328:Adult; D044466:Asian People; D001483:Base Sequence; D002681:China / Q000208:ethnology; D005260:Female; D005787:Gene Frequency; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D017354:Point Mutation; D020641:Polymorphism, Single Nucleotide; D019718:Receptors, CXCR4 / Q000235:genetics*
D019718:Receptors, CXCR4