[Regulation of XW630 on estrogen receptor messenger RNA expression in osteoblasts].
This study was performed to investigate the mechanism of an anti-osteoporosis new drug XW630 for promoting the osteogenic action. Osteoblast clone was cultivated from SD adult rat calvaria in vitro, the estrogen receptor messenger RNA(ERmRNA) expression in the osteoblasts of rats was detected directly with high-sensitive RT-PCR firstly. The results indicated that XW630 can significantly promote ERmRNA expression in osteoblasts in the time-dependent manner. Being superior to other two kinds of estrogen in the same concentration (10(-6) mol/L), XW630 probably plays an important role in the bone pathogenesis by means of ER gene regulatory functions.
Wang
Dazhang
D
West China College of Stomotogy, Sichuan University, Chengdu 610041.
Fei
Wei
W
Zheng
Hu
H
Weng
Lingling
L
Deng
Li
L
D000818:Animals; D002478:Cells, Cultured; D005260:Female; D015870:Gene Expression; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D010879:Piperazines / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011960:Receptors, Estrogen / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013752:Tetracycline / Q000494:pharmacology*; D013754:Tetracyclines
C428143:2-(3-estrone-N-ethyl-piperazine-methyl)tetracycline; D010879:Piperazines; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D013754:Tetracyclines; D013752:Tetracycline