Inhibition of cytochrome P450 isozymes in rat liver microsomes by polysaccharides derived from Phellinus linteus.
Polysaccharides (0.5, 1 and 3 mg ml(-1)) from cultured broth and mycelia of Phellinus linteus inhibited cytochrome P450 (CYP) 1A1, CYP 1A2, CYP 2B1, and CYP 2E1 activities in rat liver microsomes. The polysaccharides from the broth of Phellinus linteus grown with 5% (v/v) mulberry extract had highest inhibitory potency for CYP 1A1, 1A2 and 2B1 activities. The most potent inhibitor of CYP 2E1 activity were the polysaccharides from the broth of Phellinus linteus grown with 10% (v/v) mulberry extract.
Shon
Yun-Hee
YH
Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University, Kyongju 780-714, Korea.
Nam
Kyung-Soo
KS
D000818:Animals; D001487:Basidiomycota / Q000737:chemistry / Q000201:enzymology; D002478:Cells, Cultured; D065607:Cytochrome P-450 Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000378:metabolism; D008862:Microsomes, Liver / Q000187:drug effects* / Q000201:enzymology*; D031625:Morus / Q000382:microbiology; D025282:Mycelium / Q000737:chemistry / Q000201:enzymology; D011134:Polysaccharides / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats
D065607:Cytochrome P-450 Enzyme Inhibitors; D007527:Isoenzymes; D011134:Polysaccharides; D003577:Cytochrome P-450 Enzyme System