Virus-based expression systems facilitate rapid target in vivo functionality validation and high-throughput screening.
Target validation is one of rate-limiting steps in the modern drug discovery. The authors developed a strategy of combining adenovirus-mediated gene transfer for efficient target functionality validation, both in vivo and in vitro, with baculovirus expression to produce sufficient quantities of protein for high-throughput screening (HTS). The incorporation of green fluorescent protein (GFP) in the adenovirus vectors accelerates recombinant adenovirus plaque purification, whereas the use of epitope and affinity tags facilitates the identification and purification of recombinant protein. In this generalized scheme, the flexible modular design of viral vectors facilitates the transition between target validation and HTS. In the example presented, functional target validation in vivo was achieved by overexpressing the target gene in cell-based models and in the mouse cortex following adenovirus-mediated gene delivery. In this context, target overexpression resulted in the accumulation of a disease-related biomarker both in vitro and in vivo. A baculovirus-based expressional system was then generated to produce enough target protein for HTS. Thus, the use of these viral expression systems represents a generalized method for rapid target functionality validation and HTS assay development, which could be applied to numerous target candidates being elucidated in gene discovery programs.
Darrow
Andrew L
AL
Bioinformatics Group Leader, Johnson & Johnson Pharmaceutical Research & Development, Welsh and McKean Roads, Spring House, PA 19477, USA.
Conway
Kelly A
KA
Vaidya
Anil H
AH
Rosenthal
Daniel
D
Wildey
Mary Jo
MJ
Minor
Lisa
L
Itkin
Zina
Z
Kong
Yan
Y
Piesvaux
Jennifer
J
Qi
Jenson
J
Mercken
Marc
M
Andrade-Gordon
Patricia
P
Plata-Salamán
Carlos
C
Ilyin
Sergey E
SE
D000256:Adenoviridae; D019295:Computational Biology; D005822:Genetic Vectors; D011994:Recombinant Proteins / Q000032:analysis*
D011994:Recombinant Proteins