title: Isolation and characterization of a new d-galactose-binding lectin from Sambucus racemosa L.
abstract: A new acidic lectin from red elder (Sambucus racemosa L.) bark has been isolated by affinity chromatography and gel filtration. Noteworthy, and in contrast to other Sambucus species, red elder bark lacks acidic non-toxic type 2 ribosome-inactivating proteins but has basic ribosome-inactivating protein activities. The new lectin (SRLbm) shows specificity for N-Ac-Galactosamine/D-Galactose and has an apparent Mr of 30,000. The N-terminal amino acid sequence displays a close homology with other lectins and B chains of non-toxic type 2 ribosome-inactivating proteins nigrins and ebulins present in other Sambucus species. SRLbm triggers red blood cell agglutination in the range 4-12 micro g/ml.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Valladolid, E-47005 Valladolid, Spain.
authors.forename: M Angeles
authors.initials: MA
authors.lastname: Rojo
authors.forename: Lucía
authors.initials: L
authors.lastname: Citores
authors.forename: Pilar
authors.initials: P
authors.lastname: Jiménez
authors.forename: J Miguel
authors.initials: JM
authors.lastname: Ferreras
authors.forename: F Javier
authors.initials: FJ
authors.lastname: Arias
authors.forename: Enrique
authors.initials: E
authors.lastname: Méndez
authors.forename: Tomás
authors.initials: T
authors.lastname: Girbés
mesh_terms: D000116:Acetylgalactosamine / Q000378:metabolism; D000595:Amino Acid Sequence; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D005690:Galactose / Q000378:metabolism*; D037161:Galectins / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D006023:Glycoproteins / Q000737:chemistry; D008969:Molecular Sequence Data; D037121:Plant Lectins / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry; D054788:Ribosome Inactivating Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D012270:Ribosomes / Q000201:enzymology; D017386:Sequence Homology, Amino Acid; D014197:Trees / Q000737:chemistry*
chemical_list: D037161:Galectins; D006023:Glycoproteins; D037121:Plant Lectins; D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; C050600:Sambucus nigra lectins; C107391:ebulin r protein, Sambucus ebulus; D054788:Ribosome Inactivating Proteins; D000116:Acetylgalactosamine; D005690:Galactose