title: Flow cytometric analysis of aneuploidy and S-phase fraction in chronic myeloid leukemia patients: role in early detection of accelerated phase.
abstract: We studied S-phase fraction (SPF) and aneuploidy in peripheral blood leucocytes of patients with chronic myeloid leukemia (CML) in chronic phase (CML-CP, n=41), accelerated phase (CML-AP, n=6), and control subjects (n=12) with an aim to find out their role in early detection of accelerated phase. The SPF and aneuploidy were studied through flow-cytometry using LT-Mod. Fit software. Mean SPF value in CML-AP (9.28+/-3.46%) and in CML-CP (4.76+/-2.30%) were significantly higher than in normal controls (0.28+/-0.21%), (P<0.005, P<0.001). CML-CP patients having higher SPF (>7%) converted to accelerated phase within 18 months of follow-up while those with lower SPF (<7%) did not. Aneuploidy was present in 34.14% of CML-CP and all patients of CML-AP whereas no control subjects showed aneuploidy. Among CML-CP patients having SPF >7%, 86% developed aneuploidy during follow-up as compared to 18.50% of CML-CP with less than 7% SPF. We conclude that peripheral blood SPF and aneuploidy could be important parameters for prediction of evolution to accelerated phase in CML patients.
authors.affiliation: Hemato-Oncology Unit, Department of Medicine, K. G's Medical College, Lucknow, India. tripathiak@satyam.net.in
authors.forename: Anil K
authors.initials: AK
authors.lastname: Tripathi
authors.forename: Rupesh
authors.initials: R
authors.lastname: Chaturvedi
authors.forename: Rizwan
authors.initials: R
authors.lastname: Ahmad
authors.forename: Mohd
authors.initials: M
authors.lastname: Asim
authors.forename: Kamal K
authors.initials: KK
authors.lastname: Sawlani
authors.forename: Manoj K
authors.initials: MK
authors.lastname: Singh
authors.forename: Payal
authors.initials: P
authors.lastname: Tripathi
authors.forename: Babu L
authors.initials: BL
authors.lastname: Tekwani
mesh_terms: D000328:Adult; D000782:Aneuploidy; D016022:Case-Control Studies; D018450:Disease Progression; D005260:Female; D005434:Flow Cytometry; D005500:Follow-Up Studies; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D007962:Leukocytes / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D016196:S Phase