title: Methanococcus jannaschii coenzyme F420 analogs contain a terminal alpha-linked glutamate.
abstract: Analyses of the F(420)s present in Methanococcus jannaschii have shown that these cells contain a series of gamma-glutamyl-linked F(420)s capped with a single, terminal alpha-linked L-glutamate. The predominant form of F(420) was designated as alpha-F(420)-3 and represented 86% of the F(420)s in these cells. Analyses of Methanosarcina thermophila, Methanosarcina barkeri, Methanobacterium thermoautotrophicum, Archaeoglobus fulgidus, and Mycobacterium smegmatis showed that they contained only gamma-glutamyl-linked F(420)s.
authors.affiliation: Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061, USA.
authors.forename: Marion
authors.initials: M
authors.lastname: Graupner
authors.forename: Robert H
authors.initials: RH
authors.lastname: White
mesh_terms: D002851:Chromatography, High Pressure Liquid; D018698:Glutamic Acid / Q000737:chemistry*; D017017:Methanococcus / Q000737:chemistry / Q000201:enzymology*; D012256:Riboflavin / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D018698:Glutamic Acid; C015084:coenzyme F420; D012256:Riboflavin