title: A water-soluble azobenzene cross-linker for photocontrol of peptide conformation.
abstract: We have designed and synthesized a water-soluble, sulfonated version of an azobenzene-based thiol-reactive cross-linker that can be introduced into peptides and proteins and act as a conformational photoswitch. The sulfonated compound is shown to effect a similar degree of conformational control on a model peptide helix system, as its nonsulfonated counterpart but can be introduced without the need for any organic cosolvent. The sulfonated azobenzene cross-linker thus expands the range of proteins to which photocontrol can be applied.
authors.affiliation: Department of Chemistry, University of Toronto, 80 St. George St., Toronto M5S 3H6, Canada.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Zhang
authors.forename: Darcy C
authors.initials: DC
authors.lastname: Burns
authors.forename: Janet R
authors.initials: JR
authors.lastname: Kumita
authors.forename: Oliver S
authors.initials: OS
authors.lastname: Smart
authors.forename: G Andrew
authors.initials: GA
authors.lastname: Woolley
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D001391:Azo Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D002942:Circular Dichroism; D003432:Cross-Linking Reagents / Q000737:chemistry*; D008956:Models, Chemical; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry*; D010777:Photochemistry; D011487:Protein Conformation; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D013816:Thermodynamics; D014867:Water / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D001391:Azo Compounds; D003432:Cross-Linking Reagents; D010455:Peptides; D014867:Water