title: Crystallization and preliminary X-ray crystallographic studies of mavicyanin from Cucurbita pepo medullosa.
abstract: Mavicyanin isolated from Cucurbita pepo medullosa is a glycosylated protein containing a single polypeptide chain of 109 amino-acid residues and is a member of the phytocyanin subclass of cupredoxins. Non-glycosylated recombinant mavicyanin, which was expressed in Escherichia coli, was crystallized by the hanging-drop vapour-diffusion method with ammonium sulfate as the precipitant at pH 5.5. The crystals belonged to the hexagonal space group P6(1) (or P6(5)), with unit-cell parameters a = 64.0, c = 245.0 A, four molecules per asymmetric unit and a solvent content of 59%. X-ray diffraction data were collected to 1.6 A resolution. To solve the structure of mavicyanin, the MAD method as well as a Patterson search method using the structure of stellacyanin as a starting model are presently being utilized.
authors.affiliation: Department of Materials Chemistry, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan.
authors.forename: Yong
authors.initials: Y
authors.lastname: Xie
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Inoue
authors.forename: Youichi
authors.initials: Y
authors.lastname: Miyamoto
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Matsumura
authors.forename: Kunishige
authors.initials: K
authors.lastname: Kataoka
authors.forename: Kazuya
authors.initials: K
authors.lastname: Yamaguchi
authors.forename: Shinnichiro
authors.initials: S
authors.lastname: Suzuki
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Kai
mesh_terms: D000645:Ammonium Sulfate / Q000737:chemistry; D018360:Crystallography, X-Ray / Q000379:methods; D004058:Diffusion; D004926:Escherichia coli / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008667:Metalloproteins / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D014675:Vegetables / Q000378:metabolism*
chemical_list: D008667:Metalloproteins; D010940:Plant Proteins; C022828:mavicyanin protein, Cucurbita pepo; C003343:stellacyanin protein, plant; D000645:Ammonium Sulfate