title: A pollen-expressed gene for a novel protein with an F-box motif that is very tightly linked to a gene for S-RNase in two species of cherry, Prunus cerasus and P. avium.
abstract: This study describes a novel F-box protein gene in the S-locus of sour cherry (Prunus cerasus) and sweet cherry (P. avium). The gene showed an S-haplotype-specific sequence polymorphism and the expression was specific to pollen. Genomic DNA blot analysis of eight sweet cherry cultivars with the probe for the F-box protein gene under low stringency conditions yielded RFLP bands specific to the S-haplotypes of each cultivar. We discuss the possibility of the gene for the F-box protein being a candidate for the male determinant of gametophytic self-incompatibility in PRUNUS:
authors.affiliation: Laboratory of Pomology, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502 Japan.
authors.forename: Hisayo
authors.initials: H
authors.lastname: Yamane
authors.forename: Kazuo
authors.initials: K
authors.lastname: Ikeda
authors.forename: Koichiro
authors.initials: K
authors.lastname: Ushijima
authors.forename: Hidenori
authors.initials: H
authors.lastname: Sassa
authors.forename: Ryutaro
authors.initials: R
authors.lastname: Tao
mesh_terms: D000595:Amino Acid Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005298:Fertility / Q000235:genetics; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011058:Pollen / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D012150:Polymorphism, Restriction Fragment Length; D027861:Prunus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D012260:Ribonucleases / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins; D012260:Ribonucleases