title: Characterization of human cd200 glycoprotein receptor gene located on chromosome 3q12-13.
abstract: An immunomodulatory membrane protein, CD200R displays an expression pattern restricted to myeloid cells in mice. It is the receptor for a ligand, CD200, expressed by a broad range of cell types. In this study, we describe the cloning and characterization of the human homologue of the CD200R gene. This gene maps closely to the CD200 gene on human chromosome 3q12-13. The human CD200R gene spans a region of 52 kb, consists of nine exons, and encodes a 348-amino-acid cell-surface protein consisting of two IgFF domains in a typical V/C2 arrangement. The 59-amino-acid cytoplasmic domain has two tyrosine residues, one of which is contained within a NPXY motif. In common with other IgSF genes, the CD200R gene can generate different protein isoforms through alternative splicing. An alternative spliceout form, which has not yet been described in mice, encodes a 188-amino-acid truncated soluble polypeptide containing only the V immunoglobulin domain. In contrast to murine CD200R protein, the human membrane-bound and soluble CD200R proteins have an insertion of 23 amino acids at position 23, encoded by exon 2, which generates a putative dihydroxyacid dehydratase domain. The splicing of exon 2 generates two new isoforms, encoding the membrane and soluble proteins but lacking the dyhydroxyacid dehydratase domain. Northern-blot analysis shows that both membrane-bound and soluble isoforms are expressed in the thymus, liver, spleen and placenta. By RT-PCR, we have analyzed the expression of the four transcript variants in human placenta, spleen, liver, brain and kidney.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Granada, 18071 Granada, Spain.
authors.forename: Jose María
authors.initials: JM
authors.lastname: Vieites
authors.forename: Raul
authors.initials: R
authors.lastname: de la Torre
authors.forename: María Angeles
authors.initials: MA
authors.lastname: Ortega
authors.forename: Trinidad
authors.initials: T
authors.lastname: Montero
authors.forename: Jose María
authors.initials: JM
authors.lastname: Peco
authors.forename: Antonio
authors.initials: A
authors.lastname: Sánchez-Pozo
authors.forename: Angel
authors.initials: A
authors.lastname: Gil
authors.forename: Antonio
authors.initials: A
authors.lastname: Suárez
mesh_terms: D000328:Adult; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D015703:Antigens, CD; D000954:Antigens, Surface / Q000235:genetics*; D015152:Blotting, Northern; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005260:Female; D015870:Gene Expression; D005796:Genes / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D064446:Orexin Receptors; D020033:Protein Isoforms / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011956:Receptors, Cell Surface; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D015703:Antigens, CD; D000954:Antigens, Surface; C477337:CD200R1 protein, human; D018076:DNA, Complementary; D064446:Orexin Receptors; D020033:Protein Isoforms; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; C042739:antigens, CD200