Application of MEKC for determination of ticarcillin and clavulanic acid in Timentin intravenous preparation.
A micellar electrokinetic chromatography method for simultaneous assay of ticarcillin and clavulanic acid in Timentin i.v. injection preparation was developed. This method ensures excellent separation of both components of Timentin preparation. The validation of the method was performed, and specificity, reproducibility, precision and accuracy were confirmed. The detection and quantitative limits for Timentin were established in the concentrations 0.04 and 0.08 mg/ml, respectively. The elaborated technique was compared with two methods routinely used-UV and high performance liquid chromatography (HPLC). The obtained results and their statistical analysis proved the same precision of all methods, however, no significant differences were observed between CE and HPLC.
Pajchel
Genowefa
G
Antibiotics and Microbiology Department, National Institute of Public Health, 30134 Chelmska St., 00-725 Warsaw, Poland. tyski@il.waw.pl
Tyski
Stefan
S
D000900:Anti-Bacterial Agents / Q000032:analysis / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods; D019818:Clavulanic Acid / Q000032:analysis / Q000737:chemistry*; D002969:Clavulanic Acids / Q000008:administration & dosage / Q000032:analysis / Q000737:chemistry*; D007275:Injections, Intravenous; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013982:Ticarcillin / Q000008:administration & dosage / Q000032:analysis / Q000737:chemistry*
D000900:Anti-Bacterial Agents; D002969:Clavulanic Acids; D019818:Clavulanic Acid; C043215:ticarcillin-clavulanic acid; D013982:Ticarcillin