title: Continuous cultures of Clostridium acetobutylicum: culture stability and low-grade glycerol utilisation.
abstract: Continuous cultures of two strains of Clostridium acetobutylicum were stable for over 70 d when grown on glucose/glycerol mixtures. Butanol was the major fermentation end-product, accounting for 43 to 62% (w/w) of total products. Low-grade glycerol [65% (w/v) purity] could replace commercial glycerol [87% (w/v) purity], leading to a similar fermentation pattern: a butanol yield of 0.34 (mol/mol), a butanol productivity of 0.42 g l(-1) h(-1) and a 84% (w/w) glycerol consumption were attained when cultures were grown at pH 6 and D = 0.05 h(-1); butanol accounted for 94% (w/w) of total solvents. These values are among the highest reported in literature for C. acetobutylicum simple chemostats.
authors.affiliation: Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Porto, Portugal.
authors.forename: José Carlos
authors.initials: JC
authors.lastname: Andrade
authors.forename: Isabel
authors.initials: I
authors.lastname: Vasconcelos
mesh_terms: D019149:Bioreactors; D000440:Butanols / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods; D003013:Clostridium / Q000145:classification / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D005990:Glycerol / Q000145:classification / Q000378:metabolism*; D011786:Quality Control
chemical_list: D000440:Butanols; D005947:Glucose; D005990:Glycerol