[Distribution of microfilaments in detached corn root cap cells under fluorescent microscope].
The detached root cap cells from maize seedlings comprised of round-shaped cells, ellipse-shaped cells and longitude-shaped cells. By using fluorescein-iso-thiocyanate-phalloidin(FITC-Ph) as fluorescent probe and treatment with cytochalasin B (CB) or HMC toxin, a host-specific toxin from Bipolaris (Helminthosporium) maydis race C, the distribution and variation of microfilaments (MFs) in detached cells were investigated. The results were as follows (i) the round-shaped cells had intense fluorescence, but the network of MFs was not distinct. There was clear MFs network in the cytoplasm of both ellipse-shaped and longitude-shaped cells. The distribution of MFs in detached cells seemed to be relevant to their shape and vigor. (ii) the fluorescence of detached cells of Charrua cytoplasmic male sterility(cms-C) maize was decreased after treatment with HMC-toxin. The cause of this outcome was unclear. We only obtained the pictures of the distorted protoplast membrane and dead cells owing to treatment with HMC-toxin. The distribution of MFs of detached cells of Normal(N) cytoplasmic maize was not affected by HMC-toxin, and their protoplasts shank slightly. (iii) CB could change the distribution of MFs in detached cells of both cms-C maize and N maize to disordered arrangement.
Wang
Li An
LA
College of Life Sciences, Hebei Normal University, Shijiazhuang 050016.
Zhao
Jun Xia
JX
Ma
Chun Hong
CH
Wei
Jian Kun
JK
D008841:Actin Cytoskeleton / Q000648:ultrastructure*; D008856:Microscopy, Fluorescence; D018517:Plant Roots / Q000166:cytology / Q000648:ultrastructure; D003313:Zea mays / Q000166:cytology* / Q000648:ultrastructure