Observation of DNA damage of human hepatoma cells irradiated by heavy ions using comet assay.
OBJECTIVE
Now many countries have developed cancer therapy with heavy ions, especially in GSI (Gesellschaft f r Schwerionenforschung mbH, Darmstadt, Germany), remarkable results have obtained, but due to the complexity of particle track structure, the basic theory still needs further researching. In this paper, the genotoxic effects of heavy ions irradiation on SMMC-7721 cells were measured using the single cell gel electrophoresis (comet assay). The information about the DNA damage made by other radiations such as X-ray, gamma-ray, UV and fast neutron irradiation is very plentiful, while little work have been done on the heavy ions so far. Hereby we tried to detect the reaction of liver cancer cells to heavy ion using comet assay, meanwhile to establish a database for clinic therapy of cancer with the heavy ions.


METHODS
The human hepatoma cells were chosen as the test cell line irradiated by 80 Mev/u (20)Ne(10+) on HIRFL (China), the radiation-doses were 0, 0.5, 1, 2, 4 and 8 Gy, and then comet assay was used immediately to detect the DNA damages, 100-150 cells per dose-sample (30-50 cells were randomly observed at constant depth of the gel). The tail length and the quantity of the cells with the tail were put down. EXCEL was used for statistical analysis.


RESULTS
We obtained clear images by comet assay and found that SMMC-7721 cells were all damaged apparently from the dose 0.5 Gy to 8 Gy (t-test: P<0.001, vs control). The tail length and tail moment increased as the doses increased, and the number of cells with tails increased with increasing doses. When doses were higher than 2 Gy, nearly 100 % cells were damaged. Furthermore, both tail length and tail moment, showed linear equation.


CONCLUSIONS
From the clear comet assay images, our experiment proves comet assay can be used to measure DNA damages by heavy ions. Meanwhile DNA damages have a positive correlation with the dose changes of heavy ions and SMMC-7721 cells have a great radiosensitivity to (20)Ne(10+). Different reactions to the change of doses indicate that comet assay is a useful tool to detect DNA damage induced by heavy ions.
Qiu
Li-Mei
LM
Institute of Modern Physics, the Chinese Academy of Sciences, Lanzhou 730000, Gansu Province, China. qiuqiu69@sina.com
Li
Wen-Jian
WJ
Pang
Xin-Yue
XY
Gao
Qing-Xiang
QX
Feng
Yan
Y
Zhou
Li-Bin
LB
Zhang
Gao-Hua
GH
D006528:Carcinoma, Hepatocellular; D045744:Cell Line, Tumor / Q000502:physiology / Q000528:radiation effects; D020552:Comet Assay; D004249:DNA Damage; D004273:DNA, Neoplasm / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D007554:Isotopes / Q000494:pharmacology*; D008113:Liver Neoplasms; D009356:Neon / Q000494:pharmacology*
D004273:DNA, Neoplasm; D007554:Isotopes; D009356:Neon