Establishment of a new canine cell line (CCT) originated from a cutaneous malignant histiocytosis.
A new canine cell line, named CCT, was established from the cutaneous malignant histiocytosis in a 4-year-old male Borzoi. CCT proliferated with loose adherence and doubling time was approximately 30 hr. When co-cultured with latex beads, CCT phagocytized beads vigorously. Lysozyme and vimentin were positive by immunostaining, and non-specific esterase and acid phosphatase were positive by cytochemical staining. These features indicated the cells had a histiocytic nature. Furthermore, by subcutaneous injection to nude mice CCT could successfully form tumors with the morphological and immunohistochemical features similar to the original tumor.
Sakai
Hiroki
H
Department of Veterinary Pathology, Faculty of Agriculture, Gifu University, Gifu, Japan.
Nakano
Hiroko
H
Yamaguchi
Ryouji
R
Yonemaru
Kayoko
K
Yanai
Tokuma
T
Masegi
Toshiaki
T
D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line / Q000473:pathology*; D004285:Dogs; D015620:Histiocytic Disorders, Malignant / Q000473:pathology* / Q000662:veterinary*; D008297:Male; D012867:Skin / Q000473:pathology; D012878:Skin Neoplasms / Q000473:pathology* / Q000662:veterinary*