title: Simultaneous determination of residual antiparasitic lactones in bovine muscle and liver by liquid chromatography with fluorescence detection.
abstract: Abamectin, doramectin, eprinomectin, ivermectin, milbemectin, and moxidectin in bovine muscle and liver were extracted with acetonitrile. The extracts were partitioned with n-hexane and then evaporated to dryness. The residue was cleaned up on Bond Elute NH2 cartridge, and the drugs were eluted from the cartridge with methanol-ethyl acetate (3 + 7). The eluate was evaporated to dryness, and residues were derivatized with N,N-dimethylformamide-acetic anhyride-1-methylimidazole. The derivatives were determined by liquid chromatography with fluorescence detection. Recoveries of the 6 drugs were 79.6-63.8% in muscle and 71.6-60.6% in liver at 0.01 ppm levels. The quantitation limits were 5 ppb for each drug.
authors.affiliation: The Public Health Laboratory of Chiba Prefecture, 666-2, Nitona-cho, Chuo-ku, Chiba City 260-7815, Japan. t.ngt2@mc.pref.chiba.jp
authors.forename: Tomoko
authors.initials: T
authors.lastname: Nagata
authors.forename: Fumio
authors.initials: F
authors.lastname: Miyamoto
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Hasegawa
authors.forename: Eiichi
authors.initials: E
authors.lastname: Ashizawa
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis; D000977:Antiparasitic Agents / Q000032:analysis*; D002417:Cattle; D002853:Chromatography, Liquid; D004350:Drug Residues / Q000032:analysis*; D005453:Fluorescence; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000032:analysis; D007783:Lactones / Q000032:analysis*; D008099:Liver / Q000737:chemistry*; D018942:Macrolides; D009132:Muscles / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D000977:Antiparasitic Agents; D007783:Lactones; D018942:Macrolides; C048324:abamectin; D007559:Ivermectin; C101434:eprinomectin; C084101:doramectin; C027837:moxidectin