Glucose-dependent insulinotropic peptide stimulates proliferation and TGF-beta release from MG-63 cells.
Glucose-dependent insulinotropic peptide (GIP) is known to modulate alkaline phosphatase activity and collagen type I message in osteoblastic-like cells. GIP effects on cell proliferation are not known. We report that GIP dose dependently stimulated 3H-thymidine incorporation in the osteoblastic-like cell line MG-63. Furthermore, GIP increased message and secretion of transforming growth factor beta (TGF-beta), an agent known to regulate osteoblastic proliferation and differentiation. However, when GIP was added to MG-63 cells concurrently with a TGF-beta neutralizing antibody, there was no effect on 3H-thymidine incorporation in these cells. These data demonstrate that GIP stimulates osteoblastic-like cell proliferation but that this effect is not mediated by TGF-beta.
Zhong
Qing
Q
Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta 30912, USA.
Ding
Ke-Hong
KH
Mulloy
Anthony L
AL
Bollag
Roni J
RJ
Isales
Carlos M
CM
D000469:Alkaline Phosphatase / Q000378:metabolism; D015152:Blotting, Northern; D002454:Cell Differentiation; D002455:Cell Division; D045744:Cell Line, Tumor; D003094:Collagen / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005934:Glucagon; D052216:Glucagon-Like Peptide 1; D004763:Glucagon-Like Peptides; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D010006:Osteoblasts / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism; D010455:Peptides / Q000737:chemistry; D012313:RNA / Q000378:metabolism; D013936:Thymidine / Q000737:chemistry; D016212:Transforming Growth Factor beta / Q000378:metabolism*
D007328:Insulin; D010446:Peptide Fragments; D010455:Peptides; D016212:Transforming Growth Factor beta; C055453:glucagon-like peptide 1 (7-36)amide; D004763:Glucagon-Like Peptides; D012313:RNA; D052216:Glucagon-Like Peptide 1; D003094:Collagen; D005934:Glucagon; D000469:Alkaline Phosphatase; D005947:Glucose; D013936:Thymidine