Unnatural amino acid mutagenesis in mapping ion channel function.
Unnatural amino acid mutagenesis makes possible the site-specific incorporation of synthetic amino acids, enabling detailed structure-function studies as well as the incorporation of biophysical probes. This method has been adapted for use with heterologous expression in Xenopus oocytes, allowing experiments on ion channels.
Beene
Darren L
DL
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA.
Dougherty
Dennis A
DA
Lester
Henry A
HA
D000596:Amino Acids / Q000737:chemistry* / Q000502:physiology; D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D006801:Humans; D007473:Ion Channels / Q000737:chemistry* / Q000502:physiology; D016297:Mutagenesis, Site-Directed / Q000502:physiology*; D009865:Oocytes; D013329:Structure-Activity Relationship; D014981:Xenopus
D000596:Amino Acids; D007473:Ion Channels