The intramural innervation of the rabbit upper urinary tract.
The role of the autonomic innervation of the upper urinary tract for pyeloureteral motility is not completely understood. It is still debatable if the autonomic nervous system might play a modulating role on the ureteral peristalsis. The aim of this study was to investigate the distribution and regional variation of the intramural innervation using whole-mount preparations of the rabbit upper urinary tract. Whole-mount preparation was performed at upper urinary tracts of healthy rabbits. Immunohistochemistry was employed using Neurofilament (NF), Tyrosine Hydroxylase (TH), Choline Acetyltransferase (ChAT) and Substance P (SP) antibodies. NADPH-diaphorase and Acetylcholinesterase (AChE) histochemistry was carried out at the specimens. The stains were evaluated using brightfield, fluorescence and laser confocal microscopy. NF-, TH-, ChAT- and SP-immunoreactive (-IR) nerves formed distinct neuronal plexuses at the submucosal and muscle layers. Perivascular TH-, ChAT- and SP-IR fibres were demonstrated. AChE positive nerves were revealed in all layers, but only moderate NADPH-diaphorase positive innervation was found. Renal pelvis, upper and lower ureter showed enriched intrinsic innervation. Ganglia were found at the ureteropelvic border and the distal ureter. Whole-mount preparation technique revealed detailed informations about morphology and regional variation of the intramural innervation of the rabbit upper urinary tract.
Rolle
Udo
U
Department of Paediatric Surgery, University of Leipzig, Oststr. 21-25, 04317 Leipzig, Germany. udorolle@yahoo.com
Brylla
Elke
E
Chertin
Boris
B
Cascio
Salvatore
S
Puri
Prem
P
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D002795:Choline O-Acetyltransferase / Q000378:metabolism*; D007150:Immunohistochemistry; D009252:NADPH Dehydrogenase / Q000378:metabolism; D011817:Rabbits; D013373:Substance P / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism; D014551:Urinary Tract / Q000294:innervation* / Q000378:metabolism
D013373:Substance P; D014446:Tyrosine 3-Monooxygenase; D009252:NADPH Dehydrogenase; D002795:Choline O-Acetyltransferase; D000110:Acetylcholinesterase