Crystallization and preliminary X-ray analysis of a four-copper laccase from Coriolus hirsutus.
Laccase from the fungus Coriolus hirsutus has been purified. Crystals of the enzyme suitable for X-ray structure analysis have been obtained under optimized crystallization conditions using polyethylene glycol as a precipitant. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 50.65, b = 74.01, c = 124.83 A, and contain 40% solvent and a single molecule of laccase in the asymmetric unit. X-ray data were collected to 1.85 A at the copper edge and the four copper sites have been located from the anomalous signal. The obtained SAD phases with subsequent density modification produced a promising initial electron-density map.
Pegasova
Tatiana V
TV
A. N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky Prospekt 33, 119071 Moscow, Russia.
Zwart
Petrus
P
Koroleva
Olga V
OV
Stepanova
Elena V
EV
Rebrikov
Denis V
DV
Lamzin
Victor S
VS
D001487:Basidiomycota / Q000201:enzymology*; D003300:Copper / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D004583:Electrons; D042845:Laccase / Q000737:chemistry*; D010084:Oxidation-Reduction; D010088:Oxidoreductases; D011092:Polyethylene Glycols / Q000737:chemistry
D011092:Polyethylene Glycols; D003300:Copper; D010088:Oxidoreductases; D042845:Laccase