Subcellular localization of poly(ADP-ribose) glycohydrolase in mammalian cells.
Posttranslational modification plays important roles in a range of cellular functions. Poly(ADP-ribosyl)ation influences DNA repair, transcription, centrosome duplication, and chromosome stability. Poly(ADP-ribose) attached to acceptor proteins should be properly hydrolyzed by poly(ADP-ribose) glycohydrolase (PARG). However the subcellular localization and the role of PARG have not been well characterized. Here, we transiently expressed GFP- or Myc-tagged human PARG in mammalian cells and revealed that the subcellular distribution of human PARG changes dramatically during the cell cycle. GFP-hPARG is found almost exclusively in the nucleus during interphase. During mitosis, most GFP-hPARG protein localizes to the cytoplasm and hardly any GFP-hPARG protein is found associated with the chromosomes. Furthermore, we found that GFP-hPARG localizes to the centrosomes during mitosis. Our findings suggest that shuttling of PARG between nucleus and cytoplasm and proper control of poly(ADP-ribose) metabolism throughout the cell cycle may play an important role in regulating cell cycle progression and centrosome duplication.
Ohashi
Sayaka
S
Department of Biochemistry and Molecular Oncology, Institute of Basic Medical Sciences, University of Tsukuba, 1-1-1, Tennoudai, Tsukuba Science City, Ibaraki 305-8575, Japan.
Kanai
Masayuki
M
Hanai
Shuji
S
Uchiumi
Fumiaki
F
Maruta
Hideharu
H
Tanuma
Sei-ichi
S
Miwa
Masanao
M
D016475:3T3 Cells; D000818:Animals; D019556:COS Cells; D002453:Cell Cycle; D002467:Cell Nucleus / Q000737:chemistry; D018385:Centrosome / Q000737:chemistry*; D003593:Cytoplasm / Q000737:chemistry; D005822:Genetic Vectors; D006026:Glycoside Hydrolases / Q000032:analysis* / Q000235:genetics; D049452:Green Fluorescent Proteins; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics; D051379:Mice; D011993:Recombinant Fusion Proteins / Q000032:analysis; D014407:Tumor Cells, Cultured
D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D006026:Glycoside Hydrolases; C022407:poly ADP-ribose glycohydrolase