[Antibody response following Campylobacter infections determined by ELISA].
An enzyme-linked immunosorbent assay (ELISA) was adapted to measure immunoglobulin G (IgG), IgM, and IgA classes of human serum antibody to Campylobacter jejuni and Campylobacter coli. A total of 631 sera from 210 patients with verified Campylobacter enteritis were examined at various intervals after infection, and a control group of 164 sera were tested to determine the cut-off for negative results. With 90 percentile specificity, IgG, IgM, and IgA showed a sensitivity of 71, 60, and 80%, respectively. By combining all three antibody classes, the sensitivity was 92% within 35 days after infection, whereas within 90 days after infection, a combined sensitivity of 90% was found (IgG 68%, IgM 52%, and IgA 76%). Furthermore, we showed that 16% of the patients developed rheumatological symptoms after their Campylobacter gastrointestinal infection. We conclude that measurement of Campylobacter antibodies is a useful diagnostic tool to determine Campylobacter infections preceeding Guillain-Barré syndrome and for the investigation of post-enteritis reactive arthritis.
Engberg
Jørgen H
JH
Statens Serum Institut, Afdeling for Gastrointestinale og Parasitaere Infektioner og Afdeling for Epidemiologisk Forskning, Center for Epidemiologisk Grundforskning.
Strid
Mette Aagaard
MA
Mølbak
Kåre
K
Krogfelt
Karen Angeliki
KA
D000293:Adolescent; D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D002169:Campylobacter Infections / Q000276:immunology*; D017000:Campylobacter coli / Q000276:immunology*; D016123:Campylobacter jejuni / Q000276:immunology*; D002648:Child; D004751:Enteritis / Q000276:immunology* / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007070:Immunoglobulin A / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D008875:Middle Aged
D000907:Antibodies, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M