title: The surface receptor is involved in annexin I-stimulated insulin secretion in MIN6N8a cells.
abstract: This study investigated the effect of extracellular annexin I on regulating insulin secretion in MIN6N8a (an insulin secreting cell line) cells. The properties of annexin I receptor in MIN6N8a cells were also determined. Annexin I stimulated insulin release in MIN6N8a cells, regardless of the presence or absence of extracellular Ca(2+). Confocal microscopy revealed that annexin I bound to the surface of MIN6N8a cells. In addition, FACs analysis showed that annexin I bound to the surface of MIN6N8a cells in a dose-dependent manner. However, the annexin I-stimulated insulin secretion and the annexin I binding were abolished in MIN6N8a cells treated with proteases. Annexin I receptors were regenerated time-dependently. Furthermore, annexin I-stimulated insulin secretion was inhibited by cycloheximide but not by actinomycin D. These results showed that annexin I binds to the surface receptor in order to regulate the stimulation of insulin release in MIN6N8a cells.
authors.affiliation: Department of Biological Sciences and Institute for Basic Sciences, Sungkyunkwan University, Suwon, South Korea.
authors.forename: Jong Hak
authors.initials: JH
authors.lastname: Won
authors.forename: Na-na
authors.initials: NN
authors.lastname: Kang
authors.forename: Chung-Kyoon
authors.initials: CK
authors.lastname: Auh
authors.forename: Young Min
authors.initials: YM
authors.lastname: Park
mesh_terms: D000818:Animals; D017305:Annexin A1 / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D002469:Cell Separation; D003513:Cycloheximide / Q000378:metabolism; D003609:Dactinomycin / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D051379:Mice; D011485:Protein Binding; D011500:Protein Synthesis Inhibitors / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism*
chemical_list: D017305:Annexin A1; D007328:Insulin; D011500:Protein Synthesis Inhibitors; D011956:Receptors, Cell Surface; D003609:Dactinomycin; D003513:Cycloheximide; D002118:Calcium