Determination of phloroglucinol in human plasma by high-performance liquid chromatography-mass spectrometry.
A sensitive and selective liquid chromatographic method coupled with mass spectrometry (LC-MS) was developed for the quantification of phloroglucinol in human plasma. Resorcinol was used as internal standard, with plasma samples extracted using ethyl acetate. A centrifuged upper layer was then evaporated and reconstituted with mobile phase. The reconstituted samples were injected into a C(18) XTerra MS column (2.1 x 100 mm) with 3.5-microm particle size. The analytical column lasted for at least 500 injections. The mobile phase was 15% acetonitrile (pH 3.0), with flow-rate at 200 microl/min. The mass spectrometer was operated in negative ion mode with selective ion monitoring (SIM). Phloroglucinol was detected without severe interferences from plasma matrix when used negative ion mode. Phloroglucinol produced a parent molecule ([M-H](-)) at m/z 125 in negative ion mode. Detection of phloroglucinol in human plasma was accurate and precise, with quantification limit at 5 ng/ml. This method has been successfully applied to a study of phloroglucinol in human specimens.
Kim
Hohyun
H
Department of Pharmacokinetics, Seoul Medical Science Institute, Seoul Clinical Laboratories, 7-14, Dongbinggo-dong, Yongsan-gu, Seoul 140-809, South Korea. novakims@yahoo.com
Roh
Hyeongjin
H
Lee
Hee Joo
HJ
Chung
Soo Youn
SY
Choi
Sun Ok
SO
Lee
Kyung Ryul
KR
Han
Sang Beom
SB
D000328:Adult; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008297:Male; D010696:Phloroglucinol / Q000097:blood* / Q000493:pharmacokinetics; D012015:Reference Standards; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D010696:Phloroglucinol