Simultaneous analysis of phytohormones, phytotoxins, and volatile organic compounds in plants.
Phytohormones regulate the protective responses of plants against both biotic and abiotic stresses by means of synergistic or antagonistic actions referred to as signaling crosstalk. A bottleneck in crosstalk research is the quantification of numerous interacting phytohormones and regulators. The chemical analysis of salicylic acid, jasmonic acid, indole-3-acetic acid, and abscisic acid is typically achieved by using separate and complex methodologies. Moreover, pathogen-produced phytohormone mimics, such as the phytotoxin coronatine (COR), have not been directly quantified in plant tissues. We address these problems by using a simple preparation and a GC-MS-based metabolic profiling approach. Plant tissue is extracted in aqueous 1-propanol and mixed with dichloromethane. Carboxylic acids present in the organic layer are methylated by using trimethylsilyldiazomethane; analytes are volatilized under heat, collected on a polymeric absorbent, and eluted with solvent into a sample vial. Analytes are separated by using gas chromatography and quantified by using chemical-ionization mass spectrometry that produces predominantly [M+H]+ parent ions. We use this technique to examine levels of COR, phytohormones, and volatile organic compounds in model systems, including Arabidopsis thaliana during infection with Pseudomonas syringae pv. tomato DC3000, corn (Zea mays) under herbivory by corn earworm (Helicoverpa zea), tobacco (Nicotiana tabacum) after mechanical damage, and tomato (Lycopersicon esculentum) during drought stress. Numerous complex changes induced by pathogen infection, including the accumulation of COR, salicylic acid, jasmonic acid, indole-3-acetic acid, and abscisic acid illustrate the potential and simplicity of this approach in quantifying signaling crosstalk interactions that occur at the level of synthesis and accumulation.
Schmelz
Eric A
EA
Center of Medical, Agricultural, and Veterinary Entomology, U. S. Department of Agriculture-Agricultural Research Service, 1600/1700 Southwest 23rd Drive, Gainesville, FL 32608, USA. eschmelz@gainesville.ufl.edu
Engelberth
Juergen
J
Alborn
Hans T
HT
O'Donnell
Phillip
P
Sammons
Matt
M
Toshima
Hiroaki
H
Tumlinson
James H
JH
D000040:Abscisic Acid / Q000032:analysis; D000596:Amino Acids / Q000032:analysis; D017360:Arabidopsis / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology; D003517:Cyclopentanes / Q000032:analysis; D005030:Ethylenes / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007192:Indenes / Q000032:analysis; D007210:Indoleacetic Acids / Q000032:analysis; D009822:Oils, Volatile / Q000032:analysis; D054883:Oxylipins; D010937:Plant Growth Regulators / Q000032:analysis* / Q000096:biosynthesis / Q000502:physiology; D010944:Plants / Q000737:chemistry / Q000378:metabolism*; D015203:Reproducibility of Results; D020156:Salicylic Acid / Q000032:analysis; D015398:Signal Transduction; D021241:Spectrometry, Mass, Electrospray Ionization; D014118:Toxins, Biological / Q000032:analysis; D014835:Volatilization
D000596:Amino Acids; D003517:Cyclopentanes; D005030:Ethylenes; D007192:Indenes; D007210:Indoleacetic Acids; D009822:Oils, Volatile; D054883:Oxylipins; D010937:Plant Growth Regulators; D014118:Toxins, Biological; C058487:coronatine; C011006:jasmonic acid; C030737:indoleacetic acid; D000040:Abscisic Acid; C036216:ethylene; D020156:Salicylic Acid