DNA damage and stress transcripts in Saccharomyces cerevisiae mutant sgs1.
The human aging diseases Werner and Bloom syndromes are a result of mutation of the WRN and BLM genes, respectively. The SGS1 gene of Saccharomyces cerevisiae is homologous to the human WRN and BLM genes of the RecQ DNA helicase family. Deletion of SGS1 results in accelerated yeast aging and a reduction in life span as well as cell cycle arrest. We demonstrate that SGS1 deletion, DNA damage, and stress show similar transcriptional responses in yeast. Our comparative analysis of the genome-wide expression response of SGS1 deletion, stress and DNA damage indicates parallel transcriptional responses to cellular insult and aging in yeast.
Fry
Rebecca C
RC
Department of Biological Engineering, Massachusetts Institute of Technology, 56-211, 77 Massachusetts Avenue, Cambridge, MA 02139, USA. rfry@mit.edu
Sambandan
T G
TG
Rha
ChoKyun
C
D000375:Aging / Q000502:physiology*; D004249:DNA Damage / Q000502:physiology*; D004265:DNA Helicases / Q000235:genetics*; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D009997:Osmotic Pressure; D012333:RNA, Messenger / Q000378:metabolism; D053484:RecQ Helicases; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins; D014158:Transcription, Genetic / Q000502:physiology
D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; C090472:SGS1 protein, S cerevisiae; D004265:DNA Helicases; D053484:RecQ Helicases