Characterization of a J domain gene of Spodoptera litura multicapsid nucleopolyhedrovirus.
Spodoptera litura multicapsid nucleopolyhedrovirus (SpltMNPV) ORF39 (designated as the bjdp gene) is the only J domain gene recorded in baculovirus genomes to date. Computer-assisted analysis revealed that its deduced amino acid sequences possessed a coiled-coil region and a RNA recognition motif (RRM). In vivo the bdjp gene transcription was detected from 24 h post infection (p.i.) and abundant from 72 to 96 h p.i., while in vitro it was first detected 6 h p.i. and was abundant from 12 to 24 h p.i., respectively. Western blot analysis of BJDP expression with an antiserum made against 6xHis tagged BJDP expressed in Escherichia coli showed that it was present from 24 through 96 h p.i. in vitro. Western blot analysis indicated that BJDP was found to be associated with budded and occlusion-derived virions (ODV). Furthermore, BJDP was shown to be present in both envelope and nucleocapsid fractions of ODV. These results suggested that BJDP might be a structural protein.
Wang
Lihua
L
State Key Laboratory for Biocontro and Institute of Entomology, Zhongshan University, Guangzhou 510275, PR China.
Yu
Jianxiu
J
Yin
Chong
C
Li
Zhaofei
Z
Hu
Xiaohui
X
Pang
Yi
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D007118:Immunoassay; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D017434:Protein Structure, Tertiary; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018411:Spodoptera / Q000821:virology*