title: Are results of fibrinogen measurements transferable?
abstract: Fibrinogen concentration is routinely measured by several methods and the results may influence diagnostic and treatment strategies. It is therefore necessary that results are compatible and transferable between laboratories. In the present study, it is shown that commonly used immuno-nephelometric methods, a commercial variant of the Clauss clotting rate method, and the classical syneresis method, do not differ significantly using patient material, in the interval 2-12 g/l. A research ELISA method that measures intact fibrinogen is not linearly correlated to the syneresis method. The commutability of available calibrators and reference materials (including the WHO 2nd IS) was only 50-80% except for one of the calibrators for which the virtual concentration coincided with that obtained by the syneresis method.
authors.affiliation: Department of Surgical Sciences, Coagulation Research, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Anders
authors.initials: A
authors.lastname: Kallner
authors.forename: Nils
authors.initials: N
authors.lastname: Egberg
authors.forename: Anna
authors.initials: A
authors.lastname: Yu
authors.forename: Rumjana
authors.initials: R
authors.lastname: Merzoug-Djilali
authors.forename: Inger
authors.initials: I
authors.lastname: Blomberg
authors.forename: Gun
authors.initials: G
authors.lastname: Jörneskog
authors.forename: Margareta
authors.initials: M
authors.lastname: Blombäck
mesh_terms: D002138:Calibration; D002624:Chemistry, Clinical / Q000379:methods / Q000592:standards*; D005340:Fibrinogen / Q000032:analysis*; D006801:Humans; D007753:Laboratories / Q000592:standards / Q000706:statistics & numerical data; D016014:Linear Models; D011786:Quality Control; D012015:Reference Standards; D012016:Reference Values
chemical_list: D005340:Fibrinogen