Genetic and environmental contributions to severe preeclampsia: lack of association with the endothelial nitric oxide synthase Glu298Asp variant in a developing country.
OBJECTIVE
We recently identified a missense variant (Glu298Asp) within exon 7 of the endothelial nitric oxide synthase (eNOS) gene that is associated with severe preeclampsia in the Japanese population. Our objective was to analyze the association between the Glu298Asp eNOS gene variant and severe preeclampsia in a developing country where the incidence of preeclampsia is more frequent.


METHODS
The study included 112 Bangladeshi women exhibiting severe preeclampsia and 119 control subjects. Screening for the Glu298Asp eNOS gene variant was carried out by analysis of the polymerase chain reaction-restriction fragment length polymorphism.


RESULTS
The analysis revealed that the frequencies of the Glu298Asp variant (Glu298Asp homozygotes and heterozygotes) in the preeclampsia (n = 40; 35%) and control (n = 42; 35%) groups were not significantly (p > 0.05) different.


CONCLUSIONS
We conclude that the presence of the Glu298Asp eNOS gene is not a marker for the increased risk of preeclampsia in a population where the level of prenatal care is such that preeclampsia is still frequently seen.
Yoshimura
Toshihiro
T
Department of Obstetrics and Gynecology, Kumamoto University School of Medicine, Honjo 1-1-1, Kumamoto 860-8556, Japan. t-yoshimura@fc.kuh.kumamoto-u.ac.jp
Chowdhury
Ferdous Ara
FA
Yoshimura
Michihiro
M
Okamura
Hitoshi
H
D000328:Adult; D001224:Aspartic Acid; D001459:Bangladesh; D001724:Birth Weight; D003906:Developing Countries; D004777:Environment; D005260:Female; D005787:Gene Frequency; D014644:Genetic Variation; D018698:Glutamic Acid; D006720:Homozygote; D006801:Humans; D007231:Infant, Newborn; D019001:Nitric Oxide Synthase / Q000235:genetics*; D052250:Nitric Oxide Synthase Type III; D011225:Pre-Eclampsia / Q000201:enzymology / Q000235:genetics*; D011247:Pregnancy
D001224:Aspartic Acid; D018698:Glutamic Acid; C496314:NOS3 protein, human; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III