Feeding response of Ips paraconfusus to phloem and phloem metabolites of Heterobasidion annosum-inoculated ponderosa pine, Pinus ponderosa.
In studies of feeding by the bark beetle, Ips paraconfusus, two pine stilbenes (pinosylvin and pinosylvin methyl ether), ferulic acid glucoside, and enantiomers of the four most common sugars present in ponderosa pine phloem (sucrose, glucose, fructose, and raffinose) did not stimulate or reduce male feeding when assayed on wet alpha-cellulose with or without stimulatory phloem extractives present. When allowed to feed on wet alpha-cellulose containing sequential extracts (hexane, methanol, and water) of ponderosa pine phloem, methanol and water extractives stimulated feeding, but hexane extractives did not. Males confined in wet alpha-cellulose containing aqueous or organic extracts of culture broths derived from phloem tissue and containing the root pathogen. Heterobasidion annosum, ingested less substrate than beetles confined to control preparations. In an assay using logs from uninoculated ponderosa pines, the mean lengths of phloem in the digestive tracts increased as time spent feeding increased. Males confined to the phloem of basal logs cut from ponderosa pines artificially inoculated with H. annosum ingested significantly less phloem than beetles in logs cut from trees that were (combined) mock-inoculated or uninoculated and did not contain the pathogen. However, individual pathogen-containing treatments were not significantly different from uninoculated controls. It was concluded that altered feeding rates are not a major factor which may explain why diseased ponderosa pines are colonized by I. paraconfusus.
McNee
William R
WR
Division of Insect Biology, University of California, Berkeley, California 94720, USA. bill.mcnee@dnr.state.wi.us
Bonello
Pierluigi
P
Storer
Andrew J
AJ
Wood
David L
DL
Gordon
Thomas R
TR
D000818:Animals; D001487:Basidiomycota / Q000472:pathogenicity*; D001681:Biological Assay; D002241:Carbohydrates / Q000032:analysis; D001517:Coleoptera / Q000472:pathogenicity / Q000502:physiology; D003373:Coumaric Acids / Q000032:analysis; D005247:Feeding Behavior; D005960:Glucosides / Q000032:analysis; D008297:Male; D028223:Pinus / Q000737:chemistry* / Q000382:microbiology*; D041604:Pinus ponderosa; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D011157:Population Dynamics; D013267:Stilbenes / Q000032:analysis
D002241:Carbohydrates; D003373:Coumaric Acids; D005960:Glucosides; D010936:Plant Extracts; D013267:Stilbenes; C004999:ferulic acid