title: Chronic interstitial pneumonitis in dogs naturally infected with Leishmania (Leishmania) chagasi: a histopathological and morphometric study.
abstract: Eighteen mongrel dogs of unknown age and naturally infected with Leishmania (Leishmania) chagasi, were obtained from the City Hall of Belo Horizonte, Brazil. Four dogs were used as control. Lung samples were obtained and immediately fixed in formalin. The histopathological picture of all lung tissue sections was a chronic and diffuse interstitial pneumonitis. The thickened inter-alveolar septa were characterized by the cellular exudate (mostly macrophages, lymphocytes and plasmocytes) associated with collagen deposition. Morphometric analysis showed greater septal thickness in the infected animals than in controls. In fact, the morphometric study of collagen stained with ammoniac silver confirmed a larger deposition of collagen in the infected animals. The parasitologic method was carried out during the study of the lesions on the slides. However, we did not observe any correlation between the histopathologic and morphometric data and the clinical status of the animals. We conclude that the pulmonary lesions observed in all naturally infected dogs were correlated with the disease and that the morphometric method used was satisfactory for the analysis of septal thickness and of increased collagen deposition, confirming the presence of fibrosis.
authors.affiliation: Departamento de Cl nica e Cirurgia Veterin rias, Escola de Veterin ria, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-901, Brazil. pepe@icb.ufmg.br
authors.forename: Ricardo
authors.initials: R
authors.lastname: Gonçalves
authors.forename: Washington Luiz
authors.initials: WL
authors.lastname: Tafuri
authors.forename: Maria Norma de
authors.initials: MN
authors.lastname: Melo
authors.forename: Pedro
authors.initials: P
authors.lastname: Raso
authors.forename: Wagner Luiz
authors.initials: WL
authors.lastname: Tafuri
mesh_terms: D000818:Animals; D002908:Chronic Disease; D004283:Dog Diseases / Q000469:parasitology / Q000473:pathology*; D004285:Dogs; D005260:Female; D007891:Leishmania; D007898:Leishmaniasis, Visceral / Q000473:pathology / Q000662:veterinary*; D017563:Lung Diseases, Interstitial / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D008297:Male