Abscisic acid and gibberellin differentially regulate expression of genes of the SNF1-related kinase complex in tomato seeds.
The SNF1/AMP-activated protein kinase subfamily plays central roles in metabolic and transcriptional responses to nutritional or environmental stresses. In yeast (Saccharomyces cerevisiae) and mammals, activating and anchoring subunits associate with and regulate the activity, substrate specificity, and cellular localization of the kinase subunit in response to changing nutrient sources or energy demands, and homologous SNF1-related kinase (SnRK1) proteins are present in plants. We isolated cDNAs corresponding to the kinase (LeSNF1), regulatory (LeSNF4), and localization (LeSIP1 and LeGAL83) subunits of the SnRK1 complex from tomato (Lycopersicon esculentum Mill.). LeSNF1 and LeSNF4 complemented yeast snf1 and snf4 mutants and physically interacted with each other and with LeSIP1 in a glucose-dependent manner in yeast two-hybrid assays. LeSNF4 mRNA became abundant at maximum dry weight accumulation during seed development and remained high when radicle protrusion was blocked by abscisic acid (ABA), water stress, far-red light, or dormancy, but was low or undetected in seeds that had completed germination or in gibberellin (GA)-deficient seeds stimulated to germinate by GA. In leaves, LeSNF4 was induced in response to ABA or dehydration. In contrast, LeSNF1 and LeGAL83 genes were essentially constitutively expressed in both seeds and leaves regardless of the developmental, hormonal, or environmental conditions. Regulation of LeSNF4 expression by ABA and GA provides a potential link between hormonal and sugar-sensing pathways controlling seed development, dormancy, and germination.
Bradford
Kent J
KJ
Department of Vegetable Crops, One Shields Avenue, University of California, Davis, California 95616-8631, USA. kjbradford@ucdavis.edu
Downie
A Bruce
AB
Gee
Oliver H
OH
Alvarado
Veria
V
Yang
Hong
H
Dahal
Peetambar
P
D000040:Abscisic Acid / Q000494:pharmacology*; D000595:Amino Acid Sequence; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D005816:Genetic Complementation Test; D018525:Germination / Q000187:drug effects; D005875:Gibberellins / Q000494:pharmacology*; D018551:Solanum lycopersicum / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D008969:Molecular Sequence Data; D005810:Multigene Family; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D020798:Two-Hybrid System Techniques
D005875:Gibberellins; D012333:RNA, Messenger; D000040:Abscisic Acid; C071570:SNF1-related protein kinases; D017346:Protein Serine-Threonine Kinases