Effects of Microcystis cells, cell extracts and lipopolysaccharide on drinking and liver function in rainbow trout Oncorhynchus mykiss Walbaum.
Liver mass (hepatosomatic index, HSI) increased by approximately 18% and water content in the gut by approximately 13 ml kg(-1) in freshwater rainbow trout exposed for 24 h to intact cells of a microcystin-producing cyanobacterium (Microcystis PCC 7813) together with administration of heterotrophic bacterial LPS. Exposure to broken (ultrasonicated) cyanobacterial cells together with administration of bacterial LPS increased HSI by approximately 50% and water content in the gut by almost 30 ml kg(-1). Exposure to broken or unbroken Microcystis cells without administration of bacterial LPS resulted in increased water content of the gut (by approximately 13 ml kg(-1)) with insignificant changes in HIS. Drinking rate increased with increasing dosage of bacterial LPS alone. The increased volume of water in the gut potentially increases the opportunity for uptake of waterborne toxins, including microcystins, and increased liver mass is a symptom consistent with the toxic effects of microcystins. It is concluded that exposure of fish to the cell contents of cyanobacteria (e.g. Microcystis PCC 7813) promotes osmoregulatory imbalance resulting from stimulation of the drinking response, increased volume of fluid in the gut and inability to remove excess water.
Best
J H
JH
Division of Environmental and Applied Biology, School of Life Sciences, University of Dundee, DD1 4HN Dundee, UK.
Eddy
F B
FB
Codd
G A
GA
D000818:Animals; D001427:Bacterial Toxins / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004326:Drinking / Q000187:drug effects*; D004347:Drug Interactions; D004781:Environmental Exposure; D005260:Female; D007422:Intestines / Q000473:pathology; D008070:Lipopolysaccharides / Q000633:toxicity*; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008297:Male; D052998:Microcystins; D046931:Microcystis; D017686:Oncorhynchus mykiss / Q000502:physiology*; D009929:Organ Size / Q000187:drug effects; D010456:Peptides, Cyclic / Q000633:toxicity*; D014871:Water Microbiology; D014882:Water-Electrolyte Balance / Q000187:drug effects
D001427:Bacterial Toxins; D008070:Lipopolysaccharides; D052998:Microcystins; D010456:Peptides, Cyclic; C078588:microcystin