title: DNA: a programmable force sensor.
abstract: Direct quantification of biomolecular interaction by single-molecule force spectroscopy has evolved into a powerful tool for materials and life sciences. We introduce an approach in which the unbinding forces required to break intermolecular bonds are measured in a differential format by comparison with a known reference bond (here, a short DNA duplex). In addition to a marked increase in sensitivity and force resolution, which enabled us to resolve single-base pair mismatches, this concept allows for highly specific parallel assays. This option was exploited to overcome cross-reactions of antibodies in a protein biochip application.
authors.affiliation: Nanotype GmbH, Lochhamer Schlag 12, 82166 Gräfelfing, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Albrecht
authors.forename: Kerstin
authors.initials: K
authors.lastname: Blank
authors.forename: Mio
authors.initials: M
authors.lastname: Lalic-Mülthaler
authors.forename: Siegfried
authors.initials: S
authors.lastname: Hirler
authors.forename: Thao
authors.initials: T
authors.lastname: Mai
authors.forename: Ilka
authors.initials: I
authors.lastname: Gilbert
authors.forename: Susanne
authors.initials: S
authors.lastname: Schiffmann
authors.forename: Tom
authors.initials: T
authors.lastname: Bayer
authors.forename: Hauke
authors.initials: H
authors.lastname: Clausen-Schaumann
authors.forename: Hermann E
authors.initials: HE
authors.lastname: Gaub
mesh_terms: D000818:Animals; D000906:Antibodies; D020137:Base Pair Mismatch; D015374:Biosensing Techniques; D002232:Carbocyanines; D003429:Cross Reactions; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004129:Dimethylpolysiloxanes; D005453:Fluorescence; D005456:Fluorescent Dyes; D005898:Glass; D006801:Humans; D007118:Immunoassay; D015848:Interleukin-5 / Q000032:analysis / Q000276:immunology; D051379:Mice; D018625:Microscopy, Atomic Force; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D040081:Protein Array Analysis; D011485:Protein Binding; D012828:Silicones; D013696:Temperature; D013816:Thermodynamics
chemical_list: D000906:Antibodies; D002232:Carbocyanines; D004129:Dimethylpolysiloxanes; D005456:Fluorescent Dyes; D015848:Interleukin-5; D009838:Oligodeoxyribonucleotides; D012828:Silicones; C085321:cyanine dye 5; C013830:baysilon; D004247:DNA