DNA ploidy of bladder cancer using bladder biopsy supernate specimens.
OBJECTIVE
To perform DNA image cytometry on 119 bladder biopsy supernate (BBS) specimens of transitional cell carcinoma (TCC) bladder to: (1) test the suitability of this cytologic specimen for use in DNA ploidy analysis, and (2) assess the value of DNA ploidy measured on this specimen as to the risk of tumor recurrence and survival.


METHODS
The histologic grade and cytologic grade were correlated, and the DNA ploidy produced was determined by image analysis of Feulgen-stained nuclei. Kaplan-Meier curves related age, sex, grade and DNA ploidy to recurrence of tumor and survival. Log rank analyses were used to ascertain the difference between the curves for each categorical variable.


RESULTS
Urothelial cells derived from the BBS specimen were demonstrated to be representative of the tumor. The tumor recurrence rate was significantly higher (P = .0001) and the survival rate significantly lower (P = .0002) for patients with aneuploid tumors compared to those with diploid tumors. Patients with TCC 2 tumors had a significantly shorter time to recurrence (P = .003), although the relationship between ploidy and survival in this group was of marginal significance.


CONCLUSIONS
The specimen was free of many of the problems associate with the other specimen types used for measuring DNA ploidy. The results show that the BBS specimen is diagnostically useful and suitable for DNA analysis, providing prognostically relevant information.
Loughman
Noel T
NT
Cytology Unit, Department of Anatomical Pathology, Concord Repatriation General Hospital, Concord, NSW 2139, Australia.
Lin
Betty P C
BP
Dent
Owen F
OF
Newland
Ronald C
RC
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D002295:Carcinoma, Transitional Cell / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D002467:Cell Nucleus / Q000473:pathology; D003584:Cytological Techniques; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics*; D005260:Female; D006801:Humans; D019044:Image Cytometry / Q000379:methods; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local; D011003:Ploidies; D011379:Prognosis; D015203:Reproducibility of Results; D012394:Rosaniline Dyes; D015996:Survival Rate; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology
D004273:DNA, Neoplasm; C007722:Feulgen stain; D012394:Rosaniline Dyes