High circulating levels of cytokines (IL-6 and TNFalpha), adhesion molecules (VCAM-1 and ICAM-1) and selectins in patients with peripheral arterial disease at rest and after a treadmill test.
Peripheral arterial disease (PAD) is a common manifestation of atherosclerosis that is associated with systemic inflammation. The aim of our study was to assess whether plasma markers of inflammation increased after exercise in patients with PAD. The study was conducted on two groups of 20 subjects each: one group (mean age 68.4 +/- 5.09 years) was affected by PAD with claudication, while the other group consisted of healthy controls (66.9 +/- 6.1 years). Concentrations of interleukin 6 (IL-6) and tumor necrosis factor alpha (TNFalpha) were determined in plasma, in supernatants and in cells stimulated with 1 mg lipopolysaccharide in all patients. E-selectin (ES), L-selectin (LS) and P-selectin (PS) concentrations and plasma concentrations of VCAM-1 and ICAM-1 were also determined. All determinations were performed in patients at rest and after the treadmill exercise. Resting values of soluble mediators were greater in PAD patients than in controls. They increased in both groups after the treadmill test, even if post-treadmill concentrations were significantly higher in PAD patients (PAD p < 0.001 or 0.0001, controls p < 0.05 or 0.001). These results confirm that white blood cell activation is characteristic of systemic atherosclerosis and that these inflammation markers increase in conditions of hemodynamic stress.
Signorelli
Salvatore Santo
SS
Department of Internal Medicine and Medical Specialties, Faculty of Medicine, University of Catania, Catania, Italy. ssignore@unict.it
Mazzarino
Maria Clorinda
MC
Di Pino
Luigi
L
Malaponte
Grazia
G
Porto
Carmela
C
Pennisi
Giuseppe
G
Marchese
Giuseppe
G
Costa
Maria Pia
MP
Digrandi
Daniele
D
Celotta
Gabriella
G
Virgilio
Vittorio
V
D000368:Aged; D000970:Antineoplastic Agents / Q000097:blood*; D001157:Arterial Occlusive Diseases / Q000097:blood* / Q000209:etiology / Q000503:physiopathology*; D001161:Arteriosclerosis / Q000097:blood* / Q000150:complications / Q000503:physiopathology*; D015444:Exercise / Q000502:physiology*; D005080:Exercise Test; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood*; D015850:Interleukin-6 / Q000097:blood*; D008297:Male; D008875:Middle Aged; D016491:Peripheral Vascular Diseases / Q000097:blood* / Q000209:etiology / Q000503:physiopathology*; D012146:Rest / Q000502:physiology*; D019087:Selectins / Q000097:blood*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*; D019010:Vascular Cell Adhesion Molecule-1 / Q000097:blood*
D000970:Antineoplastic Agents; D018836:Inflammation Mediators; D015850:Interleukin-6; D019087:Selectins; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1