title: Determination of omeprazole in bulk and injectable preparations by liquid chromatography.
abstract: An accurate, simple, reproducible, and sensitive liquid chromatographic method was developed and validated for the determination of omeprazole in powder for injection and in pellets. The analyses were performed at room temperature on a reversed-phase C18 column of 250 x 4.6 mm id, 5 microm particle size. The mobile phase, composed of methanol-water (90 + 10, v/v), was pumped at a constant flow rate of 1.5 mL/min. Detection was performed on a UV detector at 301 nm. The method was validated in terms of linearity, precision, accuracy, and ruggedness. The response was linear in the range 32-48 microg/mL (r2 = 0.9976). The relative standard deviation values for intra- and interday precision studies were 1.22 and 1.56% for injectable and 2.13 and 2.45% for pellets, respectively. Recoveries ranged between 95.81 and 100.48%.
authors.affiliation: Universidade Federal de Santa Maria, Departamento de Farmácia Industrial, Santa Maria, RS, Brazil, CEP 97105-900.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Schubert
authors.forename: Almeci L
authors.initials: AL
authors.lastname: Werle
authors.forename: Cleber A
authors.initials: CA
authors.lastname: Schmidt
authors.forename: Cristiane
authors.initials: C
authors.lastname: Codevilla
authors.forename: Lisiane
authors.initials: L
authors.lastname: Bajerski
authors.forename: Ricardo
authors.initials: R
authors.lastname: Chiappa
authors.forename: Simone G
authors.initials: SG
authors.lastname: Cardoso
mesh_terms: D002853:Chromatography, Liquid; D007267:Injections; D009853:Omeprazole / Q000032:analysis*
chemical_list: D009853:Omeprazole