Identification of differentially expressed genes in coronary atherosclerotic plaques from patients with stable or unstable angina by cDNA array analysis.
The composition of atherosclerotic plaques is a crucial factor in determining rupture, thrombosis and clinical events. In this study, we analyzed gene expression in coronary plaques from patients with stable or unstable angina using gene arrays. Total RNA was extracted from eight plaques collected by therapeutic directional coronary atherectomy. cDNA probes, generated by amplification, were hybridized to nylon arrays containing 482 genes. Here we report the results for the inflammation, adhesion and hemostasis subsets. Many genes not previously associated with atherosclerosis, such as the lymphocyte adhesion molecule MadCAM, were expressed in the plaques. anova analysis showed higher tissue factor (TF) expression in unstable angina samples. Five genes were expressed at lower levels in unstable angina samples: anticoagulant protein S, cyclooxygenase (COX)-1, interleukin (IL)-7 and chemokines monocyte chemotactic protein (MCP)-1 and -2. Gene arrays provide a new approach to study plaque composition and identify candidate markers of plaque instability.
Randi
A M
AM
Experimental Medicine, GlaxoSmithKline, Stevenage, UK. a.randi@ic.ac.uk
Biguzzi
E
E
Falciani
F
F
Merlini
P
P
Blakemore
S
S
Bramucci
E
E
Lucreziotti
S
S
Lennon
M
M
Faioni
E M
EM
Ardissino
D
D
Mannucci
P M
PM
D000787:Angina Pectoris / Q000235:genetics / Q000473:pathology*; D016000:Cluster Analysis; D003324:Coronary Artery Disease / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D007249:Inflammation / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D013927:Thrombosis / Q000235:genetics