title: Isolation and characterization of maltokinase (ATP:maltose 1-phosphotransferase) from Actinoplanes missouriensis.
abstract: Crude extracts of Actinoplanes missouriensis and related strains catalyze the ATP-dependent phosphorylation of maltose to maltose 1-phosphate. The enzyme of A. missouriensis responsible for this reaction was purified and characterized. This protein has an estimated molecular mass of 57 kDa and it is most likely a monomer. The K(m) value was 2.6 mM for maltose and 0.54 mM for ATP. Only maltose acted effectively as phosphoryl-group acceptor, and ATP was not replaceable as phosphoryl-group donor. Tryptic peptides of the enzyme were sequenced, and the sequences of these peptides will allow construction of degenerate primers to identify the gene coding for this unique kinase.
authors.affiliation: Westfälische Wilhelms-Universität, Institut für Mikrobiologie, Corrensstrasse 3, 48149, Münster, Germany.
authors.forename: Birgit
authors.initials: B
authors.lastname: Niehues
authors.forename: Ralf
authors.initials: R
authors.lastname: Jossek
authors.forename: Uwe
authors.initials: U
authors.lastname: Kramer
authors.forename: Anne
authors.initials: A
authors.lastname: Koch
authors.forename: Martin
authors.initials: M
authors.lastname: Jarling
authors.forename: Werner
authors.initials: W
authors.lastname: Schröder
authors.forename: Hermann
authors.initials: H
authors.lastname: Pape
mesh_terms: D007700:Kinetics; D008320:Maltose / Q000378:metabolism*; D008848:Micromonosporaceae / Q000201:enzymology*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000737:chemistry / Q000302:isolation & purification*; D013379:Substrate Specificity; D013403:Sugar Phosphates / Q000378:metabolism
chemical_list: D013403:Sugar Phosphates; C080751:maltose 1-phosphate; D008320:Maltose; D017853:Phosphotransferases (Alcohol Group Acceptor); C100489:maltokinase