[Inhibition by CTLA-4Ig on herpetic stromal keratitis in a murine model].
OBJECTIVE
To investigate the inhibition by CTLA-4Ig on herpetic stromal keratitis (HSK).


METHODS
BALB/c mice infected with HSV-1 Mckrae strain by corneal scarification were injected intraperitoneally with murine CTLA-4Ig given on days 0, +2, +4 after the infection. The effects of CTLA-4Ig on HSK were evaluated.


RESULTS
As measured by flow cytometry, in the mice treated with CTLA-4Ig, 81.6% of CD4(+)T cells and 67.9% of CD8(+)T cells were reduced. CTLA-4Ig halted the onset of HSK, reduced the corneal stromal opacification of HSK, prevented extensive cellular infiltration in cornea, impaired the delayed type hypersensitivity and inhibited splenocytes producing Th1 cytokines.


CONCLUSIONS
The results provide evidence that blockade of B7:CD28/CTLA-4 costimulation by CTLA-4Ig can inhibit the proliferation of T cells and Th1 response and impair onset and severity of HSK.
Xia
Li-kun
LK
Department of Ophthalmology, The Second Affiliated Hospital, China Medical University, Shenyang 110004, China. xialk@online.ln.cn
Zhang
Jin-song
JS
Shu
Hong
H
D000069594:Abatacept; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects; D003319:Corneal Stroma / Q000276:immunology / Q000473:pathology; D018796:Immunoconjugates / Q000494:pharmacology*; D007155:Immunologic Factors / Q000494:pharmacology*; D016849:Keratitis, Herpetic / Q000188:drug therapy* / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
D018796:Immunoconjugates; D007155:Immunologic Factors; D000069594:Abatacept