Conjugation of heparin into carboxylated pullulan derivatives as an extracellular matrix for endothelial cell culture.
A carboxylated pullulan, for use as a structural material for a number of tissue engineering applications, was synthesized and conjugated with heparin. By immobilization of heparin to pullulan, endothelial cells (ECs) attached on the heparin-conjugated pullulan were more aggregated than when attached to other pullulan derivatives. Attachments were 50, 45, 49, and 90% for a polystyrene dish, pullulan acetate, carboxylated pullulan, and heparin-conjugated pullulan, respectively. Heparin-conjugated pullulan inhibited the proliferation of smooth muscle cells (SMCs) in vitro. Heparin-conjugated pullulan material can thus be used for the proliferation of vascular ECs and to inhibit the proliferation of SMCs.
Na
Kun
K
College of Pharmacy, Chosun University, 375 Seoseok-Dong Dong-Ku, Kwangju 501-759, Korea.
Shin
Dongchul
D
Yun
Kyusik
K
Park
Keun-Hong
KH
Lee
Kang Choon
KC
D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002449:Cell Aggregation / Q000502:physiology; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D018914:Cells, Immobilized / Q000502:physiology; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis; D004730:Endothelium, Vascular / Q000502:physiology*; D005109:Extracellular Matrix / Q000737:chemistry* / Q000502:physiology; D005936:Glucans / Q000737:chemistry*; D006493:Heparin / Q000737:chemistry*; D006801:Humans; D046911:Macromolecular Substances; D008567:Membranes, Artificial; D009131:Muscle, Smooth, Vascular / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods
D020099:Coated Materials, Biocompatible; D005936:Glucans; D046911:Macromolecular Substances; D008567:Membranes, Artificial; C009109:pullulan; D006493:Heparin