Isolation of a new member of the ADP-ribosylation like factor gene family, ARL8, from a cartilage cDNA library.
ADP-ribosylation factors (ARFs) and ARF-like proteins (ARLs) are part of the ARF family within the RAS superfamily of regulatory GTPases. Guanine nucleotide binding proteins or GTPases are involved in a diverse spectrum of cellular activities, including regulating cell growth and signal transduction, organization of the cytoskeleton and regulating membrane trafficking along the exocytic and endocytic pathways. ARL proteins share 40-60% sequence identity with the ARF proteins, but ARLs can be distinguished from ARFs based on expression patterns and biological functions. We have identified a new ARL, ARL8, from a fetal cartilage cDNA library. ARL8 contains six exons and five introns, and encodes a 179 amino acid protein that shares homology to the other ARL proteins, especially ARL5. It also shows significant homology with orthologous proteins found in Mus musculus and Drosophila melanogaster. The expression pattern of the mouse ortholog revealed differential tissue expression and an alternate transcript was seen in brain that was age-dependent. ARL8 is an additional member of a family of closely related proteins that are conserved both within the family and across species.
Sebald
Eiman
E
Department of Obstetrics and Gynecology, Burns and Allen Cedars-Sinai Research Institute, 8700 Beverly Boulevard, Los Angeles, CA 90048, USA.
Krueger
Richard
R
King
Lily M
LM
Cohn
Daniel H
DH
Krakow
Deborah
D
D020727:ADP-Ribosylation Factors / Q000235:genetics*; D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D002356:Cartilage / Q000378:metabolism*; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10 / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005091:Exons; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D005796:Genes / Q000235:genetics; D006801:Humans; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D026801:Synteny
D018076:DNA, Complementary; C477339:ARL5B protein, human; D020727:ADP-Ribosylation Factors