Identification of androgen-regulated genes in SV40-transformed human hair dermal papilla cells.
BACKGROUND
Androgens are major regulators of human hair growth, which exert their effect on follicular epithelium via the mesenchyme-derived dermal papilla (DP) cells. However, very few data are available with regard to the genes regulated by androgen in DP cells.


OBJECTIVE
To investigate the differentially expressed genes by androgen in DP cells.


METHODS
Human hair DP cells were cultured and transformed with SV40 T antigen. Using cDNA representational difference analysis, androgen-regulated genes in SV40-transformed dermal papilla (SDP) cells were identified.


RESULTS
SDP cells revealed extended lifespan as compared with primary cultured DP cells. SDP cells expressed androgen receptor (AR) and showed androgen responsiveness. cDNA representational difference analysis followed by Northern blot analysis showed that heat shock cognate protein, Hsc70 was differentially expressed by dihydrotestosterone (DHT) treatment in SV40-transformed DP cells.


CONCLUSIONS
These results suggest that Hsc70 may be involved in androgen action on DP cells.
Kim
Chang Deok
CD
Oriental Hospital, Oriental Medical College of Daejeon University, 22-5 Daeheung-dong, 301-724, Daejeon, South Korea.
Lee
Min Ho
MH
Roh
Seok Seon
SS
D000728:Androgens / Q000494:pharmacology / Q000502:physiology*; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D013196:Dihydrotestosterone / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D018859:Hair Follicle / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D011944:Receptors, Androgen / Q000378:metabolism; D013539:Simian virus 40
D000728:Androgens; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; C494371:HSPA8 protein, human; D011944:Receptors, Androgen; D013196:Dihydrotestosterone