Casein hydrolysis in PEG-salt two phase system by Eudragit-chymotrypsin bioconjugate.
A bioconjugate of alpha-chymotrypsin and Eudragit S-100 was used in an aqueous two-phase system (polyethylene glycol/phosphate) for casein hydrolysis. More product was obtained by replacing the lower salt phase with a fresh one during the reaction. The bioconjugate could be reused six times for casein hydrolysis.
Teotia
Sunita
S
Chemistry Department, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi 110016, India.
Sharma
Shweta
S
Gupta
M N
MN
D000327:Adsorption; D002364:Caseins; D002918:Chymotrypsin / Q000737:chemistry*; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000737:chemistry; D006868:Hydrolysis; D046911:Macromolecular Substances; D008567:Membranes, Artificial; D010710:Phosphates / Q000737:chemistry; D011092:Polyethylene Glycols; D011109:Polymethacrylic Acids / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D002364:Caseins; D004800:Enzymes, Immobilized; D046911:Macromolecular Substances; D008567:Membranes, Artificial; D010710:Phosphates; D011109:Polymethacrylic Acids; C038300:methylmethacrylate-methacrylic acid copolymer; D011092:Polyethylene Glycols; D002918:Chymotrypsin