title: An extracellular Bacillus sp. chitinase for the production of chitotriose as a major chitinolytic product.
abstract: An extracellular chitinase of Bacillus sp. WY22 was purified by 9.6-fold. It had a Mr of 35 kDa, an apparent Km value for colloidal chitin of 3 mg ml(-1) and was optimally active at 37 degrees C and pH 5.5 over 1 h. The enzyme could also hydrolyse swollen chitin, glycol chitin and chitosan with relative activities of 76%, 34% and 23% compared with colloidal chitin. It formed chitotriose as a major product from colloidal chitin and glycol chitin.
authors.affiliation: Department of Food Science and Technology, Kyungpook National University, 1370 Sankyuk, Daegu 702-701, Korea.
authors.forename: Cheol-Joo
authors.initials: CJ
authors.lastname: Woo
authors.forename: Heui-Dong
authors.initials: HD
authors.lastname: Park
mesh_terms: D001407:Bacillus / Q000737:chemistry / Q000145:classification / Q000201:enzymology*; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry*; D002688:Chitinases / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D005110:Extracellular Space / Q000737:chemistry / Q000378:metabolism; D008970:Molecular Weight; D013045:Species Specificity; D013379:Substrate Specificity; D014312:Trisaccharides / Q000138:chemical synthesis*
chemical_list: D014312:Trisaccharides; D002686:Chitin; C041161:chitotriose; C031610:glycolchitin; D002688:Chitinases