title: Amyloid protofilaments from the calcium-binding protein equine lysozyme: formation of ring and linear structures depends on pH and metal ion concentration.
abstract: The calcium-binding equine lysozyme has been found to undergo conversion into amyloid fibrils during incubation in solution at acidic pH. At pH 4.5 and 57 degrees C, where equine lysozyme forms a partially unfolded molten globule state, the protein forms protofilaments with a width of ca. 2 nm. In the absence of Ca(2+) the protofilaments are present as annular structures with a diameter of 40-50 nm. In the presence of 10 mM CaCl(2) the protofilaments of equine lysozyme are straight or curved; they can assemble into thicker threads, but they do not appear to undergo circularisation. At pH 2.0, where the protein is more destabilised compared to pH 4.5, fibril formation occurs at 37 degrees C and 57 degrees C. At pH 2.0, both ring-shaped and linear protofilaments are formed, in which periodic repeats of ca 35 nm can be distinguished clearly. The rings constitute about 10% of all fibrillar species under these conditions and they are characterised by a larger diameter of 70-80 nm. All the structures bind Congo red and thioflavine T in a manner similar to fibrils associated with a variety of amyloid diseases. At pH 2.0, fibril formation is accompanied by some acidic hydrolysis, producing specific fragmentation of the protein, leading to the accumulation of two peptides in particular, consisting of residues 1-80 and 54-125. At the initial stages of incubation, however, full-length equine lysozyme represents the dominant species within the fibrils. We propose that the ring-shaped structures observed here, and in the case of disease-associated proteins such as alpha-synuclein, could be a second generic type of amyloid structure in addition to the more common linear fibrils.
authors.affiliation: Department of Biochemistry, Umeå University, Umeå SE-90187, Sweden.
authors.forename: Mantas
authors.initials: M
authors.lastname: Malisauskas
authors.forename: Vladimir
authors.initials: V
authors.lastname: Zamotin
authors.forename: Jana
authors.initials: J
authors.lastname: Jass
authors.forename: Wim
authors.initials: W
authors.lastname: Noppe
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Dobson
authors.forename: Ludmilla A
authors.initials: LA
authors.lastname: Morozova-Roche
mesh_terms: D000682:Amyloid / Q000737:chemistry*; D000818:Animals; D052160:Benzothiazoles; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism; D004396:Coloring Agents / Q000494:pharmacology; D003224:Congo Red / Q000494:pharmacology; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D006736:Horses; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007477:Ions; D018625:Microscopy, Atomic Force; D008958:Models, Molecular; D009113:Muramidase / Q000737:chemistry*; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013696:Temperature; D013844:Thiazoles / Q000737:chemistry
chemical_list: D000682:Amyloid; D052160:Benzothiazoles; D004396:Coloring Agents; D007477:Ions; D013844:Thiazoles; C009462:thioflavin T; D003224:Congo Red; D009113:Muramidase; D002118:Calcium