A novel inflammatory eye disease induced by lymphocytes from knockout mice sensitized against the deleted ocular antigen.
Lens-associated uveitis (LAU), a severe inflammatory eye disease, is thought to be mediated by autoimmunity against lens crystallins. Previously described animal models for this disease are antibody-mediated, since no cellular response to self crystallins could be induced in experimental animals. Here, we describe a new model for LAU, in which lymphocytes from knockout mice deficient in alphaB-crystallin are sensitized against the deleted protein and induce severe ocular inflammation when adoptively transferred into wild type recipients. Similar to LAU, the experimental disease developed only following rupture of the lens capsule, produced in this study by capsulotomy; no disease was detected in recipient eyes with no capsulotomy, or in those treated with cautery, or in eyes affected by systemic treatment with sodium iodate, lipopolysaccharide or X-irradiation. The ocular changes in affected eyes included heavy cellular infiltration and proteinaceous exudate in both the anterior and posterior segments of the eye, that reached their peak on day 4 following cell transfer and subsided quite rapidly thereafter.
Gelderman
M P
MP
National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892-1857, USA.
Charukamnoetkanok
P
P
Brady
J P
JP
Hung
L
L
Zigler
J S
JS
Wawrousek
E F
EF
Vistica
B P
BP
Fortin
E
E
Chan
C-C
CC
Gery
I
I
D019264:Adoptive Transfer; D000818:Animals; D017209:Apoptosis / Q000276:immunology; D001327:Autoimmune Diseases / Q000209:etiology / Q000276:immunology* / Q000473:pathology; D002478:Cells, Cultured; D003459:Crystallins / Q000235:genetics / Q000276:immunology*; D004195:Disease Models, Animal; D007111:Immunity, Cellular; D007903:Lens Capsule, Crystalline / Q000601:surgery; D051379:Mice; D018345:Mice, Knockout; D013154:Spleen / Q000276:immunology; D014605:Uveitis / Q000209:etiology / Q000276:immunology* / Q000473:pathology
D003459:Crystallins