Crystallization and preliminary crystallographic analysis of DJ-1, a protein associated with male fertility and parkinsonism.
DJ-1 was identified as a novel oncogene product that transformed mouse NIH3T3 cells in cooperation with activated Ras. DJ-1 was also correlated with male infertility and parkinsonism. DJ-1 was crystallized using sodium citrate and HEPES at pH 7.5. The crystal belongs to space group P3(1) or P3(2), with unit-cell parameters a = 75.04, c = 74.88 A and contains two molecules in an asymmetric unit. An intensity data set was collected to 2.00 A resolution.
Honbou
Kazuya
K
Department of Structural Biology, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan.
Suzuki
Nobuo N
NN
Horiuchi
Masataka
M
Taira
Takahiro
T
Niki
Takeshi
T
Ariga
Hiroyoshi
H
Inagaki
Fuyuhiko
F
D002021:Buffers; D002461:Cell Line, Transformed; D002951:Citrates / Q000494:pharmacology; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000378:metabolism; D005298:Fertility; D006531:HEPES / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D015513:Oncogene Proteins / Q000737:chemistry*; D010300:Parkinson Disease / Q000378:metabolism; D000071617:Protein Deglycase DJ-1; D000077559:Sodium Citrate; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D002021:Buffers; D002951:Citrates; D047908:Intracellular Signaling Peptides and Proteins; D015513:Oncogene Proteins; D000077559:Sodium Citrate; C105131:PARK7 protein, human; D000071617:Protein Deglycase DJ-1; D006531:HEPES