[Carcinoids of the gastrointestinal tract: importance of determining differentiation and proliferation markers].
The group of 35 carcinoid tumours obtained from 34 patients was reviewed according to recent histopathological criteria. Consequently, evaluation of the Grimelius staining and immunohistochemical detection of chromogranin A (CgA), Leu-7 (CD-57), synaptophysin, neuron-specific enolase (NSE), (beta-III tubulin, Ki-67 and proliferating cell nuclear antigen (PCNA) was performed. The majority of tumours (29, i.e. 83%) were classified as typical carcinoids composed predominantly of mixed solid and trabecular or solid and tubular growth patterns. Six tumours (17%) revealed more prominent cytological abnormalities corresponding with the diagnosis of atypical carcinoid. The majority of tumours (31, i.e. 93.9%) showed granular cytoplasmic positivity in Grimelius staining and diffuse cytoplasmic positivity of NSE (34, i.e. 97.1%). All of the 32 stained tumour samples showed positive immunoreactivity for synaptophysin. A high percentage of tumours (32, i.e. 91.4%) revealed also a positive reaction with antibody TU-20 detecting (beta-III tubulin, a marker of an early stage of neuronal differentiation. Thirty-four tumours (97.1%) showed granular cytoplasmic positivity for both markers of neuroendocrine granules (CgA and Leu-7). One tumour (2.9%) was positive only for Leu-7. Tumour cells revealed predominantly low proliferative activity evaluated by PCNA and Ki-67 immunodetection. Higher degree of proliferation was observed especially in atypical carcinoids.
Jirásek
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Ustav patologie 3. lékarské fakulty UK a Fakultní nemocnice Královské Vinohrady, Praha.
Mandys
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D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D018959:CD57 Antigens / Q000032:analysis; D002276:Carcinoid Tumor / Q000737:chemistry / Q000175:diagnosis*; D053379:Chromogranin A; D002864:Chromogranins / Q000032:analysis; D005260:Female; D005770:Gastrointestinal Neoplasms / Q000737:chemistry / Q000175:diagnosis*; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis; D008297:Male; D008875:Middle Aged; D010751:Phosphopyruvate Hydratase / Q000032:analysis; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D016708:Synaptophysin / Q000032:analysis; D014404:Tubulin / Q000032:analysis
D014408:Biomarkers, Tumor; D018959:CD57 Antigens; D053379:Chromogranin A; D002864:Chromogranins; D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen; D016708:Synaptophysin; D014404:Tubulin; D010751:Phosphopyruvate Hydratase