Conversion of an obligate autotrophic bacteria to heterotrophic growth: expression of a heterogeneous phosphofructokinase gene in the chemolithotroph Acidithiobacillus thiooxidans.
A plasmid pSDK-1 containing the Escherichia coli phosphofructokinase-1 gene (pfkA) was constructed, and transferred into Acidithiobacillus thiooxidans Tt-7 by conjugation. The pfkA gene from E. coli could be expressed in this obligately autotrophic bacterium but the enzyme activity (18 U g-1) was lower than that in E. coli (K12: 86 U g-1; DF1010 carrying plasmid pSDK-1: 97 U g-1). In the presence of glucose, the Tt-7 transconjugant consumed glucose leading to a better growth yield.
Tian
Ke Li
KL
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, P.R. China. tianzr@sdu.edu.cn
Lin
Jian Qun
JQ
Liu
Xiang Mei
XM
Liu
Ying
Y
Zhang
Chang Kai
CK
Yan
Wang Ming
WM
D003227:Conjugation, Genetic; D004789:Enzyme Activation; D004795:Enzyme Stability; D004926:Escherichia coli / Q000145:classification / Q000201:enzymology / Q000235:genetics; D020563:Gammaproteobacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D005947:Glucose / Q000378:metabolism*; D010732:Phosphofructokinase-1 / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D013045:Species Specificity
D011994:Recombinant Proteins; D010732:Phosphofructokinase-1; D005947:Glucose