title: In vitro bactericidal activities of daptomycin against Staphylococcus aureus and Enterococcus faecalis are not mediated by inhibition of lipoteichoic acid biosynthesis.
abstract: Previous studies have suggested that lipoteichoic acid biosynthesis inhibition is the mechanism of action of daptomycin. In this investigation, daptomycin inhibited all macromolecular synthesis in Staphylococcus aureus, Enterococcus faecalis, and Enterococcus hirae without kinetic or dose specificity for lipoteichoic acid. Daptomycin remained bactericidal in the absence of ongoing lipoteichoic acid synthesis. Inhibition of lipoteichoic acid synthesis is apparently not the mechanism of action of daptomycin in these pathogens.
authors.affiliation: Cubist Pharmaceuticals, Inc., Lexington, Massachusetts 02421, USA.
authors.forename: Valerie
authors.initials: V
authors.lastname: Laganas
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Alder
authors.forename: Jared A
authors.initials: JA
authors.lastname: Silverman
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017576:Daptomycin / Q000494:pharmacology*; D016983:Enterococcus / Q000187:drug effects / Q000378:metabolism; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D007700:Kinetics; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D012313:RNA / Q000096:biosynthesis; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D013682:Teichoic Acids / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000494:pharmacology; D014640:Vancomycin / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D008070:Lipopolysaccharides; D013682:Teichoic Acids; C009900:lipoteichoic acid; D012313:RNA; D014640:Vancomycin; D017576:Daptomycin