title: Hepatic ischemia-reperfusion syndrome after partial liver resection (LR): hepatic venous oxygen saturation, enzyme pattern, reduced and oxidized glutathione, procalcitonin and interleukin-6.
abstract: The hepatic ischemia-reperfusion syndrome was investigated in 28 patients undergoing elective partial liver resection with intraoperative occlusion of hepatic inflow (Pringle maneuver) using the technique of liver vein catheterization. Hepatic venous oxygen saturation (ShvO2) was monitored continuously up to 24 hours after surgery. Aspartate aminotransferase, glutamate dehydrogenase, gamma-glutamyl transpeptidase, pseudocholinesterase, alpha-glutathione S-transferase, reduced and oxidized glutathione, procalcitonine, and interleukin-6 were serially measured both before and after Pringle maneuver during the resection and postoperatively in arterial and/or hepatic venous blood. ShvO2 measurement demonstrated that peri- and postoperative management was suitable to maintain an optimal hepatic oxygen supply. As expected, we were able to demonstrate a typical enzyme pattern of postischemic liver injury. There was a distinct decrease of reduced glutathione levels both in arterial and hepatic venous plasma after LR accompanied by a strong increase in oxidized glutathione concentration during the phase of reperfusion. We observed increases in procalcitonin and interleukin-6 levels both in arterial and hepatic venous blood after declamping. Our data support the view that liver resection in man under conditions of inflow occlusion resulted in ischemic lesion of the liver (loss of glutathione synthesizing capacity with disturbance of protection against oxidative stress) and an additional impairment during reperfusion (liberation of reactive oxygen species, local and systemic inflammation reaction with cytokine production). Additionally, we found some evidence for the assumption that the liver has an export function for reduced glutathione into plasma in man.
authors.affiliation: Clinic of Anesthesiology and Intensive Care Medicine, Waldklinikum Gera gGmbH, Germany. m.kretzschmar@waldklinikumgera.de
authors.forename: Michael
authors.initials: M
authors.lastname: Kretzschmar
authors.forename: Antie
authors.initials: A
authors.lastname: Krüger
authors.forename: Wulf
authors.initials: W
authors.lastname: Schirrmeister
mesh_terms: D002116:Calcitonin / Q000097:blood*; D015740:Calcitonin Gene-Related Peptide; D005260:Female; D019803:Glutathione Disulfide / Q000097:blood*; D006498:Hepatectomy / Q000009:adverse effects* / Q000379:methods; D006801:Humans; D015850:Interleukin-6 / Q000097:blood*; D008099:Liver / Q000098:blood supply / Q000473:pathology / Q000601:surgery; D008297:Male; D008875:Middle Aged; D010100:Oxygen / Q000097:blood*; D011498:Protein Precursors / Q000097:blood*; D012039:Regional Blood Flow; D015427:Reperfusion Injury / Q000097:blood* / Q000473:pathology
chemical_list: C000606589:CALCA protein, human; D015850:Interleukin-6; D011498:Protein Precursors; D002116:Calcitonin; D015740:Calcitonin Gene-Related Peptide; D010100:Oxygen; D019803:Glutathione Disulfide