Microbial transformation of ginsenoside Rb1 by Rhizopus stolonifer and Curvularia lunata.
Of 49 microbial strains screened for their capabilities to transform ginsenoside Rb1, Rhizopus stolonifer and Curvularia lunata produced four key metabolites: 3-O-[beta-D-glucopyranosyl-(1,2)-beta-D-glucopyranosyl]-20-O-[beta-D-glucopyranosyl]-3beta,12beta, 20(S)-trihydroxydammar-24-ene (1), 3-O-[beta-D-glucopyranosyl-(1,2)-beta-D-glucopyranosyl]-20-O-[beta-D-glucopyranosyl]-3beta,12beta, 20(S)-trihydroxydammar-24-ol (2), 3-O-[beta-D-glucopyranosyl-(1,2)-beta-D-glucopyranosyl]-3beta, 12beta, 20(S)-trihydroxydammar-24-ene (3), and 3-O-beta-D-glucopyranosyl-3beta, 12beta, 20(S)-trihydroxydammar-24-ene (4), identified by TOF-MS, 1H- and 13C-NMR spectral data. Metabolites 1, 3 and 4 were from the incubation with R. stolonifer, and 1 and 2 from the incubation with C. lunata. Compound 2 was identified as a new compound.
Dong
Aling
A
The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Xueyuan Road #38, Beijing 100083, PR China.
Ye
Min
M
Guo
Hongzhu
H
Zheng
Junhua
J
Guo
Dean
D
D001203:Ascomycota / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D001711:Biotransformation; D002855:Chromatography, Thin Layer / Q000379:methods; D036145:Ginsenosides / Q000737:chemistry* / Q000378:metabolism*; D012233:Rhizopus / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D013045:Species Specificity
D036145:Ginsenosides; C442759:ginsenoside Rb1