Effects of adrenalectomy on CART expression in the rat arcuate nucleus.
In order to test for glucocorticoid regulation of CART in the arcuate nucleus, adrenalectomies (ADX) and hormone replacements (HRs) were carried out in groups of rats. CART mRNA levels were determined by in situ hybridization and CART peptide levels by immunocytochemistry. ADX caused a lowering of CART mRNA and peptides levels in the arcuate and this was reversed by HR. These results indicate a glucocorticoid regulation of CART in the arcuate. The regulation could be direct through an action of glucocorticoid receptors or indirectly through ADX-induced changes in leptin levels. These findings suggest a role for CART in the stress response.
Balkan
Burcu
B
Ege University Center for Brain Research, School of Medicine, Department of Physiology, Bornova, Izmir, Turkey.
Koylu
Ersin
E
Pogun
Sakire
S
Kuhar
Michael J
MJ
D000302:Adrenal Cortex / Q000293:injuries / Q000378:metabolism*; D000315:Adrenalectomy; D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000378:metabolism*; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D005938:Glucocorticoids / Q000172:deficiency* / Q000494:pharmacology; D007150:Immunohistochemistry; D009419:Nerve Tissue Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000187:drug effects / Q000378:metabolism; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein
D005938:Glucocorticoids; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D011965:Receptors, Glucocorticoid; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein