Angiogenesis and fibroblast growth factors (FGFs) in a three-dimensional collagen gel culture.
Small pieces of mouse aorta were cultured in collagen gels, and the formation of capillary-like tubes from the aortic explant was observed under phase-contrast and transmission electron microscopes. Migration of fibroblastic cells from the aortic explant occurred in 2 days. After about 10 days of culture, capillary-like tubes from the aortic explant were formed in the collagen gels. An immunohistochemical study on the collagen gel culture revealed the expression of fibroblast growth factor 2 (FGF-2) near the aortic explant at the active initial stage, with random migration of fibroblastic cells expressing FGF-9. mRNA was isolated from these cultures, and reverse transcription-polymerase chain reaction (RT-PCR) of the cultures revealed the expressions of both FGF-2 and FGF-9. Based on our results, we propose that FGF-9 is also related to angiogenic events.
Nagatoro
Takashi
T
Dept. of Anatomy, Biomedical Research Center, Saitama Medical School, Moroyama, Iruma-gun, Saitama 350-0495, Japan. tnagator@saitama-med.ac.jp
Fujita
Keiko
K
Murata
Eiko
E
Akita
Masumi
M
D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology / Q000378:metabolism; D002196:Capillaries / Q000648:ultrastructure; D018929:Cell Culture Techniques / Q000379:methods*; D002465:Cell Movement; D002478:Cells, Cultured; D003094:Collagen; D003470:Culture Media; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000378:metabolism*; D051525:Fibroblast Growth Factor 9; D005346:Fibroblast Growth Factors / Q000235:genetics / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005782:Gels; D007124:Immunoenzyme Techniques; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008854:Microscopy, Electron; D018919:Neovascularization, Physiologic / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D003470:Culture Media; C496460:Fgf9 protein, mouse; D051525:Fibroblast Growth Factor 9; D005782:Gels; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2; D005346:Fibroblast Growth Factors; D003094:Collagen