Characterization of the species- and stage-specificity of two monoclonal antibodies against Leishmania amazonensis.
Leishmania metacyclogenesis is associated with changes in morphology, gene expression, and structural alterations of the lipophosphoglycan (LPG), the promastigote most abundant surface glycolipid. Purification of metacyclics is accomplished using lectins or monoclonal antibodies (MAbs) that exploit stage-specific differences in the LPG. Besides, LPG displays extensive interspecies polymorphisms and is synthesized by promastigotes of all species investigated to date. In this work we studied the species- and stage-specificity of two MAbs (3A1-La and LuCa-D5) used to purify metacyclics of Leishmania amazonensis. Their ability to recognize different members of the Trypanosomatidae family was tested by direct agglutination, indirect immunofluorescence, and dot-blot analysis of LPG. We found that both MAbs were highly selective for L. amazonensis: 3A1-La recognized only promastigotes and LuCa-D5 labeled amastigote and promastigote stages of this species. These MAbs might be useful for Leishmania typing.
Chaves
C S
CS
Departamento de Imunologia, Instituto de Microbiologia-UFRJ, Rio de Janeiro, RJ, Brazil.
Soares
D C
DC
Da Silva
R P
RP
Saraiva
E M
EM
D000372:Agglutination Tests; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000913:Antibodies, Protozoan / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity; D005455:Fluorescent Antibody Technique; D006028:Glycosphingolipids / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D007891:Leishmania / Q000145:classification* / Q000254:growth & development* / Q000276:immunology; D008018:Life Cycle Stages; D051379:Mice; D008807:Mice, Inbred BALB C; D013045:Species Specificity; D014351:Trypanosomatina / Q000145:classification / Q000276:immunology
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D006028:Glycosphingolipids; C008290:lipophosphonoglycan