Biooxidation of ferrous iron by immobilized Acidithiobacillus ferrooxidans in poly(vinyl alcohol) cryogel carriers.
PVA-cryogels entrapping about 10(9) cells of Acidithiobacillus ferrooxidans per ml of gel were prepared by freezing-thawing procedure, and the biooxidation of Fe2+ by immobilized cells was investigated in a 0.365 l packed-bed bioreactor. Fe2+ oxidation fits a plug-flow reaction model well. A maximum oxidation rate of 3.1 g Fe2+ l(-1) h(-1) was achieved at the dilution rate of 0.4 h(-1) or higher, while no obvious precipitate was determined at this time. In addition, cell-immobilized PVA-cryogels packed in bioreactor maintained their oxidative ability for more than two months under non-sterile conditions.
Long
Zhong-er
ZE
State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, CAS, Beijing 100080, PR China. Longzhonger@163.com
Huang
Yunhong
Y
Cai
Zhaoling
Z
Cong
Wei
W
Ouyang
Fan
F
D019149:Bioreactors; D018914:Cells, Immobilized / Q000145:classification / Q000378:metabolism; D015925:Cryopreservation / Q000379:methods*; D005240:Feasibility Studies; D005296:Ferrous Compounds / Q000378:metabolism*; D020563:Gammaproteobacteria / Q000145:classification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008954:Models, Biological; D010084:Oxidation-Reduction; D010865:Pilot Projects; D011142:Polyvinyl Alcohol
D005296:Ferrous Compounds; C020748:ferrous sulfate; D011142:Polyvinyl Alcohol