title: The impact of early testicular sperm extraction or cryopreservation on the outcome of intracytoplasmic sperm injection--a randomized controlled study.
abstract: OBJECTIVE
  To compare the outcome of sperm extraction 24 h before ovum pickup and on the day of oocyte retrieval.
  
  
  METHODS
  A controlled study was performed to compare the outcome of 90 sperm extractions and in vitro sperm injection cycles performed in 54 patients.
  
  
  RESULTS
  Available fresh sperm for the sperm injection procedure and cryopreservation obtained on the day of ovum pickup were similar to sperm collected 1 day before (33.3% vs 39.4%, respectively). Fertilization rate obtained with fresh sperm was also similar (48.9% vs. 54%), respectively. Clinical pregnancy rate was 38% vs. 22% per embryo transfer, respectively (P = 0.235). When comparing an additional 24 cycles with cryopreservation of sperm retrieved on the day of ovum pickup, as well as a day previously, no significance was noted in the parameters.
  
  
  CONCLUSIONS
  Sperm retrieved 24 h before oocyte retrieval and used as fresh or frozen-thawed for sperm injection are as effective as those used on the day of ovum pickup.
authors.affiliation: IVF Units, Carmel Medical Center, Technion, The Rappaport School of Medicine, 34362, Haifa, Israel.
authors.forename: L
authors.initials: L
authors.lastname: Dirnfeld
authors.forename: M
authors.initials: M
authors.lastname: Paz
authors.forename: D
authors.initials: D
authors.lastname: Yshai
authors.forename: I
authors.initials: I
authors.lastname: Calderon
authors.forename: S
authors.initials: S
authors.lastname: Lahav-Baratz
authors.forename: M
authors.initials: M
authors.lastname: Koifman
authors.forename: H
authors.initials: H
authors.lastname: Shiloh
authors.forename: H
authors.initials: H
authors.lastname: Abramovici
authors.forename: D
authors.initials: D
authors.lastname: Bider
mesh_terms: D000328:Adult; D002469:Cell Separation / Q000379:methods; D015925:Cryopreservation / Q000379:methods*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods; D005640:Follicle Stimulating Hormone / Q000097:blood; D006801:Humans; D008297:Male; D009865:Oocytes / Q000166:cytology; D011247:Pregnancy; D011256:Pregnancy Outcome; D015203:Reproducibility of Results; D012662:Semen Preservation / Q000379:methods*; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa / Q000166:cytology*
chemical_list: D005640:Follicle Stimulating Hormone