title: Effects of method and MRI slice thickness on entorhinal cortex volumetry.
abstract: We assessed the effect of the method of analysis and the MRI slice thickness on entorhinal cortex volumetry. A T1 gradient echo 3D volumetric acquisition was reformatted into different slice thickness and analyzed by edge-tracing. We performed two different forms of analysis of images with 3 mm slice thickness: edge-tracing and pixel by pixel. There was difference among the volumes obtained from different slice thickness (p < 0.001), and also difference between the two different methods of analysis (p < 0.05). The use of thick slices is time saving, but volumes are linearly increased; different methods of segmentation also yields different values. The form of volumetric analysis of the entorhinal cortex should be evaluated in advance to prevent false estimates in longitudinal studies.
authors.affiliation: Laboratory of Neuroimaging, State University of Campinas, Campinas, SP, Brazil. Ibonilha@unicamp.br
authors.forename: Leonardo
authors.initials: L
authors.lastname: Bonilha
authors.forename: Eliane
authors.initials: E
authors.lastname: Kobayashi
authors.forename: Fernando
authors.initials: F
authors.lastname: Cendes
authors.forename: Li M
authors.initials: LM
authors.lastname: Li
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D001931:Brain Mapping; D018728:Entorhinal Cortex / Q000033:anatomy & histology* / Q000378:metabolism; D005260:Female; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation / Q000379:methods; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D008875:Middle Aged