Uncinaria stenocephala: antigenic characterization of larvae and adults worms using sera from naturally infected dogs.
An antigenic characterization of the larval (somatic) and adult (somatic and excretory-secretory) antigens of Uncinaria stenocephala was made, employing immunoblotting and immunoblotting-inhibition with 10 selected sera from dogs naturally infected by this hookworm. The results indicated that each one of the three parasitic extracts has different antigenic components. Sixty percent of the dog sera consistently recognised four antigens of 20, 25, 30, and 38kDa of the larval extract and the immunoblotting inhibition showed that these antigens were only observed at the larval stage. All these results indicated that these antigens can be considered as major antigens and they could be useful for the immunodiagnosis of this parasitic infection.
Postigo
I
I
Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy, University of the Basque Country, P.O. Box 450, 01080 Vitoria, Spain.
Martínez
J
J
Cardona
G
G
Fernández-Pérez
I
I
Guisantes
J A
JA
D000723:Ancylostomatoidea / Q000254:growth & development / Q000276:immunology*; D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D004283:Dog Diseases / Q000276:immunology* / Q000469:parasitology; D004285:Dogs; D015801:Helminth Proteins / Q000276:immunology; D006725:Hookworm Infections / Q000175:diagnosis / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D007106:Immune Sera / Q000276:immunology*; D015151:Immunoblotting; D007814:Larva / Q000276:immunology
D000947:Antigens, Helminth; D015801:Helminth Proteins; D007106:Immune Sera