Crystallization and preliminary X-ray analysis of alginate lyase, a member of family PL-7, from Pseudomonas aeruginosa.
Alginate lyase depolymerizes alginate, a heteropolysaccharide consisting of alpha-L-guluronate and beta-D-mannuronate, through a beta-elimination reaction. A protein PA1167 with a molecular mass of 25 kDa produced by Pseudomonas aeruginosa is an alginate lyase classified into polysaccharide lyase family PL-7. The enzyme was crystallized at 293 K in a drop solution comprising 1.4 M sodium chloride, 0.1 M potassium sodium phosphate and 0.1 M 2-morpholinoethanesulfonate-sodium hydroxide pH 6.5 by means of the vapor-diffusion method. The crystals were monoclinic and belonged to space group P2(1), with unit-cell parameters a = 43.4, b = 70.3, c = 67.4 A, beta = 94.5 degrees. Diffraction data were collected to 2.0 A from a single crystal.
Yamasaki
Masayuki
M
Division of Agronomy and Horticultural Science, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan.
Moriwaki
Satoko
S
Hashimoto
Wataru
W
Mikami
Bunzo
B
Murata
Kousaku
K
D002240:Carbohydrate Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000378:metabolism; D008956:Models, Chemical; D008969:Molecular Sequence Data; D010710:Phosphates / Q000737:chemistry; D011133:Polysaccharide-Lyases / Q000737:chemistry*; D011188:Potassium / Q000737:chemistry; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D012965:Sodium Chloride / Q000737:chemistry; D012972:Sodium Hydroxide / Q000737:chemistry; D013696:Temperature; D014965:X-Rays
D010710:Phosphates; D012965:Sodium Chloride; D012972:Sodium Hydroxide; D011133:Polysaccharide-Lyases; C016158:poly(beta-D-mannuronate) lyase; D011188:Potassium; C018279:sodium phosphate