Minisequencing on functionalised self-assembled monolayer as a simple approach for single nucleotide polymorphism analysis of cattle.
We have developed a genetic barcode module, based on a parallel sorting facility of single nucleotide polymorphism for secure individual identification of cattle. Biotinylated allele-specific oligonucleotides were immobilized onto the predefined spots of streptavidin tethered self-assembled monolayers with long chain alkanethiols on biochips. The target DNAs for hybridization and subsequent on-chip minisequencing were produced by multiplex PCR method. After enzymatic extension, only the moiety-modified dideoxynucleotide triphosphate, when coupled to its complementary target sequence, could be detected by the corresponding antibody to the moiety in a specific and sensitive manner. The database SNPZoo was developed for storage of the sequence information consisting of cytosine/thymidine patterns This SNP chip system can further be used in the detection of any replaceable point mutations occurring in the human and animal genes.
Du
Weidong
W
Antarus LifeScience GmbH, Sedanstr. 14, D-89077 Ulm, Germany.
Cieplik
Michael
M
Durstewitz
Gregor
G
Sarkar
Chandra Mohan
CM
Kruschina
Margit
M
Fries
Rudi
R
Ortigao
Flavio Ramalho
FR
D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D030561:Databases, Nucleic Acid; D005821:Genetic Techniques; D005838:Genotype; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide
D004247:DNA