title: Mutans streptococci and lactobacilli in carious dentine before and after Atraumatic Restorative Treatment.
abstract: OBJECTIVE
  This study was performed to examine the cultivable flora in dentine after manual excavation of caries lesions using the Atraumatic Restorative Treatment (ART) technique and to examine for associations between the microbiological results and cavity size, dentine colour and consistency.
  
  
  METHODS
  Dentine samples from 40 caries lesions were collected before and after treatment and cultured for total viable counts (TVC), mutans streptococci (MS) and lactobacilli.
  
  
  RESULTS
  The bacterial bioburden between the two samples showed a significant reduction in the frequency and proportions of TVC and MS but not lactobacilli.
  
  
  CONCLUSIONS
  Cavity preparation produced a clinical change in dentine colour and consistency from dark shades and soft dentine at enamel dentine junction to light shades and hard dentine at the cavity floor. The results show that cavity size, dentine colour and consistency are not absolute indicators of the microbiological bioburden in an ART cavity.
authors.affiliation: Dental Research Institute, University of the Witwatersrand, Private Bag 3, WITS 2050, Johannesburg, South Africa.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Bönecker
authors.forename: Cheryl
authors.initials: C
authors.lastname: Toi
authors.forename: Peter
authors.initials: P
authors.lastname: Cleaton-Jones
mesh_terms: D002648:Child; D002675:Child, Preschool; D015169:Colony Count, Microbial; D003731:Dental Caries / Q000382:microbiology* / Q000628:therapy; D003793:Dental Restoration, Permanent / Q000379:methods*; D003804:Dentin / Q000382:microbiology* / Q000473:pathology; D005899:Glass Ionomer Cements; D006801:Humans; D007778:Lactobacillus / Q000302:isolation & purification; D016014:Linear Models; D013295:Streptococcus mutans / Q000302:isolation & purification
chemical_list: D005899:Glass Ionomer Cements