Polymorphism of HLA-DMA and DMB alleles in patients with systemic lupus erythematosus.
OBJECTIVE
To evaluate the contribution of HLA-DM alleles to susceptibility to systemic lupus erythematosus (SLE) in a Caucasian population.


METHODS
HLA-DMA and DMB alleles were studied in 73 patients with SLE, 147 randomly selected controls, and 86 HLA-DRB1 genotype matched controls by oligotyping of polymerase chain reaction amplified genomic DNA with sequence-specific oligonucleotide probes.


RESULTS
There was a significant presence of HLA-DMA*0103, DMA*0104, and DMB*0102 in the SLE patients compared with the randomly selected controls. After stratification of patients and matched controls according to DRB1 genotypes, only HLA-DMA*0104 was increased in SLE patients negative for the SLE susceptibility HLA-DR alleles. For the patients and controls positive for HLA-DR allele-susceptibility for SLE, HLA-DMA*0103, DMA*0104, DMB*0102, and DMB*0103 alleles tended to be more frequent, but without reaching statistical significance. No correlation was found between HLA-DM phenotype frequencies and any clinical or biological manifestations of SLE.


CONCLUSIONS
This is the first study evaluating the influence of HLA-DM in a Caucasian SLE population. Our results suggest that HLA-DMA*0104 may represent a novel allele of susceptibility to SLE.
Morel
Jacques
J
Service d'Immunorhumatologie, Hôpital Lapeyronie, Montpellier, France. j-morel@chu-montpellier.fr
Simoes
Clarisse Da Silva
Cda S
Avinens
Odile
O
Sany
Jacques
J
Combe
Bernard
B
Eliaou
Jean-Francois
JF
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D004247:DNA / Q000097:blood; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006681:HLA-D Antigens / Q000235:genetics*; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D006681:HLA-D Antigens; C084245:HLA-DM antigens; D004247:DNA