Fur mutants of Pseudomonas aeruginosa PAO1: phenotypic characterization in terms of iron-dependent pattern of deregulation of pyoverdin synthesis.
Fur mutants (Mn(r)) of Pseudomonas aeruginosa Fe10 (FF13 and FF28) and PAO1 (FPA12) were evaluated for the pattern of deregulation of pyoverdin synthesis in iron-replete medium with Casamino acids [CAM(Fe)] or succinate [SM(Fe)]. With respect to siderophore synthesis, we found in CAM(Fe) medium two Fur phenotypes: FurA (full deregulation, FF13) and FurB (partial deregulation, FF28 and FPA12). Fur mutants compared to parental strains grew with slower specific growth rates on SM(0) and CAM(0) media in a stirred bioreactor. Fur mutants grew in SM(0) with mu about half of that in CAM(0).
Johnová
Andrea
A
Laboratory of Enzyme Technology, Institute of Microbiology, Academy of Sciences of the Czech Republic, 14220 Prague 4, Czech Republic.
Dobisová
Marie
M
Abdallah
M A
MA
Kyslík
Pavel
P
D000596:Amino Acids / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D007501:Iron / Q000494:pharmacology*; D016296:Mutagenesis; D009154:Mutation; D009842:Oligopeptides; D010641:Phenotype; D010860:Pigments, Biological / Q000096:biosynthesis*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D017262:Siderophores / Q000096:biosynthesis; D019802:Succinic Acid / Q000494:pharmacology
D000596:Amino Acids; D001426:Bacterial Proteins; D009842:Oligopeptides; D010860:Pigments, Biological; D012097:Repressor Proteins; D017262:Siderophores; C017721:casamino acids; C068477:ferric uptake regulating proteins, bacterial; C042453:pyoverdin; D019802:Succinic Acid; D007501:Iron