FTICR mass spectrometry in proteomics.
Analytical tools that allow rapid screening, low sample consumption and accurate protein identification are of great importance in studies of complex biological samples. Today, mass spectrometry (MS) is a key analytical tools with applications in a wide variety of fields, reaching from the analysis of elemental compositions in various materials to the identification of large protein complexes. One of the fastest growing fields of MS applications is proteomics, or the study of protein expression in an organism. In the traditional proteomic approach, two-dimensional sodium dodecylsulfate polyacrylamide gel electrophoresis is applied for the separation and visualization of proteins. In this review, the use of high resolution Fourier transform ion cyclotron resonance mass spectrometry, including up-front multidimensional liquid separations for 'top down' or 'bottom up' proteomic approaches, are presented.
Bergquist
Jonas
J
Institute of Chemistry, Department of Analytical Chemistry, Biomedical Center, Uppsala University, PO Box 599, SE-751 24 Uppsala, Sweden. jonas.bergquist@kemi.uu.se
D000818:Animals; D002853:Chromatography, Liquid; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D010455:Peptides / Q000032:analysis; D011506:Proteins / Q000032:analysis; D040901:Proteomics; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D010455:Peptides; D011506:Proteins