Adeno-associated virus mediated LacZ gene transfect to cultured human iris pigment epithelium cells.
OBJECTIVE
To study the feasibility of adeno-associated virus mediated gene transfection to cultured human iris pigment epithelium (IPE) cells in vitro.


METHODS
Recombinant replication deficient adeno-associated viruses (AAV) expressing LacZ gene were produced without helper virus. The LacZ gene was transduced into cultured human IPE cells.


RESULTS
Cultured human IPE cells stained positively anticytokeratin, The titer of rAAV-LacZ was 2.1 x 10(8) virus particles/ml, 42% cultured human IPE cells expressed beta-galactosidase 7 days after transfection and 67% after 14 days.


CONCLUSIONS
Recombined AAV produced without helper virus can transfer a foreign gene into human IPE cells with high efficiency in vitro.
Zhang
Chun
C
Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.
Tang
Shibo
S
Luo
Yan
Y
Liang
Xiaoling
X
Ma
Jing
J
Lin
Shaofen
S
D002478:Cells, Cultured; D004274:DNA, Recombinant; D000229:Dependovirus / Q000235:genetics*; D015870:Gene Expression; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006801:Humans; D007498:Iris / Q000166:cytology / Q000378:metabolism*; D007763:Lac Operon / Q000235:genetics*; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000378:metabolism*; D014162:Transfection; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D004274:DNA, Recombinant; D001616:beta-Galactosidase