Direct electrochemical characterization of Vitreoscilla sp. hemoglobin entrapped in organic films.
The redox properties of a prokaryotic, Vitreoscilla sp. hemoglobin (VHb) in fuzzy organic films are studied with electrochemistry. This VHb exhibits irreversible electrochemical response at bare pyrolytic graphite (PG) electrode surfaces. However, upon being entrapped in organic films, the heterogeneous electron transfer rate of VHb will be sufficiently high to produce a quasi-reversible electrochemical response. The observation of electrocatalysis (reduction of O2) by hemes suggests that the protein can retain its biological activity under these conditions.
Fan
Chunhai
C
Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, PR China.
Zhong
Jun
J
Guan
Rong
R
Li
Genxi
G
D001426:Bacterial Proteins / Q000737:chemistry*; D002244:Carbon; D004121:Dimethyl Sulfoxide; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D006108:Graphite; D006454:Hemoglobins / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry; D011094:Polyethyleneimine; D011994:Recombinant Proteins / Q000737:chemistry; D054793:Truncated Hemoglobins
D001426:Bacterial Proteins; D006454:Hemoglobins; D011994:Recombinant Proteins; D054793:Truncated Hemoglobins; C009139:pyrolytic carbon; C082613:hemoglobin protein, Vitreoscilla; D002244:Carbon; D006108:Graphite; D011094:Polyethyleneimine; D010100:Oxygen; D004121:Dimethyl Sulfoxide