Intra-tumoral injection of CpG results in the inhibition of tumor growth in murine Colon-26 and B-16 tumors.
Direct tumor injections of CpG (ODN #1826) into murine tumors markedly suppressed the tumor growth and increased the survival of the mice. Tumor growth was reduced by 60-67% in Colon Tumor 26 (CT-26) and B-16 melanoma tumors treated with CpG as compared to untreated one. In CT-26 and B-16 tumors treated with CpG, the average survival of the animals were prolonged to 26 and 28 d as compared to 16 and 18 d in control respectively. Long-term surviving animals in CT-26 tumor groups were also protected from a subsequent injection of a lethal dose of tumor cells. In the present study, effect of CpG was mediated through CD8+ T cells, as their depletion resulted in the abrogation of the therapeutic effects of the CpG. It suggests that direct tumor injection might be a simple means of achieving a clinical response in cancer patients.
Sharma
Sanjay
S
Department of Surgery, SUNY Buffalo and Kaleida Health, 124 Multi Research Bldg., 100 High St., Buffalo General Hospital, Buffalo, NY 14203, USA.
Karakousis
Constantine P
CP
Takita
Hiroshi
H
Shin
Kyu
K
Brooks
Stephen P
SP
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000276:immunology / Q000503:physiopathology*; D004247:DNA / Q000008:administration & dosage* / Q000276:immunology; D005260:Female; D015552:Injections, Intralesional; D008545:Melanoma / Q000188:drug therapy* / Q000276:immunology / Q000503:physiopathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009367:Neoplasm Staging / Q000379:methods; D009368:Neoplasm Transplantation; D009838:Oligodeoxyribonucleotides; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000276:immunology
C423449:CpG ODN 1826; D009838:Oligodeoxyribonucleotides; D004247:DNA