Increased urinary 15-F2t-isoprostane concentrations in patients with non-ischaemic congestive heart failure: a marker of oxidative stress.
OBJECTIVE
To investigate a novel marker of oxidative stress in patients with congestive heart failure (CHF).


METHODS
15 patients with mild CHF, 15 patients with severe CHF with acute exacerbation, and 15 control subjects.


METHODS
Measurement of urinary 15-F2t-isoprostane, plasma brain natriuretic peptide (BNP), serum interleukin 6 (IL-6), and serum thrombomodulin concentrations. In patients with severe CHF, samples were taken at admission and 4, 7, and 14 days after admission.


RESULTS
Urinary 15-F2t-isoprostane, plasma BNP, and serum IL-6 concentrations in patients with severe CHF were significantly higher than those in control subjects or in patients with mild CHF. However, concentrations of serum thrombomodulin, a marker of endothelial damage, were not different between patients with CHF and control subjects. In addition, urinary 15-F2t-isoprostane, plasma BNP, and serum IL-6 concentrations in patients with severe CHF gradually decreased in proportion to the severity of CHF during hospitalisation. Interestingly, urinary 15-F2t-isoprostane concentrations significantly correlated with plasma BNP concentrations and serum IL-6 concentrations, but not with serum thrombomodulin concentrations.


CONCLUSIONS
Urinary 15-F2t-isoprostane concentrations increased in proportion to the severity of CHF in patients. This may be caused by increased 15-F2t-isoprostane production. These findings suggest that urinary 15-F2t-isoprostane may be a marker of morbidity as well as oxidative stress in patients with CHF.
Nonaka-Sarukawa
M
M
Division of Cardiovascular Medicine, Jichi Medical School, Minamikawachi-Machi, Tochigi 329-0498, Japan.
Yamamoto
K
K
Aoki
H
H
Takano
H
H
Katsuki
T
T
Ikeda
U
U
Shimada
K
K
D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000652:urine; D028441:F2-Isoprostanes / Q000652:urine*; D005260:Female; D006333:Heart Failure / Q000209:etiology* / Q000652:urine; D006801:Humans; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D008875:Middle Aged; D020097:Natriuretic Peptide, Brain / Q000097:blood; D018384:Oxidative Stress / Q000502:physiology*; D018180:Thrombomodulin / Q000097:blood
D015415:Biomarkers; D028441:F2-Isoprostanes; D015850:Interleukin-6; D018180:Thrombomodulin; D020097:Natriuretic Peptide, Brain