Xstir polymorphism and absence of sex linkage in Xenopus laevis ME2 gene.
A fragment of ME2 cDNA from exon 2 to exon 11 was sequenced and the sequence submitted to GenBank. Analysis of the intron, probably intron 13, revealed a polymorphism which is due to the presence of tandem repetitions of Xstir elements. Genetic analysis of the parents and the offspring showed a standard distribution of intron variants. This distribution was not dependent on sex. We conclude, contrary to previous reports, that the ME2 gene is not linked to sex. Consequently, the Xstir polymorphism can be used as a tool for genetic analysis.
Mácha
J
J
Department of Animal Physiology and Developmental Biology, Faculty of Science, Charles University, Prague, Czech Republic. machaj@volny.cz
Tlapáková
T
T
Krylov
V
V
Kopský
V
V
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000378:metabolism; D005091:Exons; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers; D007438:Introns; D008291:Malate Dehydrogenase / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012737:Sex Factors; D014982:Xenopus laevis / Q000235:genetics*
D018076:DNA, Complementary; D005819:Genetic Markers; D008291:Malate Dehydrogenase; C034544:D-malate dehydrogenase (decarboxylating)