Pluripotent bone marrow cells in leukemic mice elicit enhanced immune reactivity following sheep erythrocyte administration in-vivo. A possible S-LFA3 interactive immunotherapy.
Reports concerning the compartmentwise immunoreactivity and migratory property of the bone marrow derived stem/progenitor cells have been poorly documented. The present study shows that both in normal and leukemic mice a low density group of bone marrow cells (LDC) are functionally less matured than a high density group of cells (HDC) as revealed from spontaneous E-rosetting, cytotoxic efficacy and phagocytic function against the targets which correlated well with the migratory activity of the cells from LDC to HDC compartment. This is deranged in leukaemic groups of animals. Administration of Sheep Erythrocytes (SRBC) significantly increased the CD34+ cell population as evident through flowcytometric (FACS) analysis and the above immune reactivity in leukemic mice. The results indicated that, (a) bone marrow cells comprising the major fractions of immature cells are capable of eliciting immune-reactivity against the targets in normal and (b) poorly in leukemic mice and that (c) sheep red blood cells could effectively trigger such immunological functions together with enhanced maturation dependent migration in leukemic mice. The study hints at therapeutic potentiality of SRBC or its determinant molecule TIITS or Sheep-leucocyte function antigen 3 (S-LFA3/CD 58) in stimulating the stem and progenitor cells in vivo.
Law
S
S
Immunology and Immunohaematology Lab., Dept. of Haematology, School of Tropical Medicine, Calcutta, India.
Begum
B
B
Chaudhuri
S
S
D000818:Animals; D018952:Antigens, CD34 / Q000096:biosynthesis; D001854:Bone Marrow Cells / Q000166:cytology*; D002273:Carcinogens; D002465:Cell Movement; D002469:Cell Separation; D004912:Erythrocytes / Q000378:metabolism*; D005038:Ethylnitrosourea; D005260:Female; D005434:Flow Cytometry; D007167:Immunotherapy / Q000379:methods*; D007938:Leukemia / Q000139:chemically induced / Q000628:therapy; D008297:Male; D051379:Mice; D010587:Phagocytosis; D012756:Sheep; D013234:Stem Cells / Q000166:cytology; D013997:Time Factors
D018952:Antigens, CD34; D002273:Carcinogens; D005038:Ethylnitrosourea