Expression of deleted in malignant brain tumor-1 (DMBT1) molecule in biliary epithelium is augmented in hepatolithiasis: possible participation in lithogenesis.
Deleted in malignant brain tumor-1 (DMBT1) is a mucin-like molecule participating in mucosal immune defense. Given that bovine gallbladder mucin, which accelerates cholesterol crystallization, is a DMBT1 homolog, DMBT1 expression was examined immunohistochemically in biliary epithelial cells in livers with hepatolithiasis (N = 25), primary sclerosing cholangitis (N = 7), large bile duct obstruction (N = 12), and control normal livers (N = 10). DMBT1 protein was determined in the hepatic bile samples of hepatolithiasis (N = 12) and other hepatobiliary diseases (N = 8) by immunoblot. While DMBT1 was faintly expressed in normal livers (20%), it was significantly augmented in hepatolithiasis (76%) (P < 0.05). DMBT1 was mildly expressed in primary sclerosing cholangitis and large bile duct obstruction. DMBT1 protein was detected frequently in hepatic bile samples of hepatolithiasis (50%) (P < 0.05), but in the other bile samples. The percentage of cholesterol in intrahepatic calculi was significantly higher in the patients with DMBT1-positive bile. Augmented expression and secretion of DMBT1 in intrahepatic large bile ducts in hepatolithiasis suggests its role in lithogenesis.
Sasaki
Motoko
M
Department of Human Pathology, Kanazawa University Graduate School of Medicine, Kanazawa, 920-8640, Japan.
Huang
Shiu-Feng
SF
Chen
Miin-Fu
MF
Jan
Yi-Yin
YY
Yeh
Ta-Sen
TS
Ishikawa
Akira
A
Mollenhauer
Jan
J
Poustka
Annemarie
A
Tsuneyama
Koichi
K
Nimura
Yuji
Y
Oda
Koji
K
Nakanuma
Yasuni
Y
D000373:Agglutinins; D001652:Bile Ducts / Q000378:metabolism* / Q000503:physiopathology; D001653:Bile Ducts, Intrahepatic; D002135:Calcium-Binding Proteins; D002769:Cholelithiasis / Q000737:chemistry / Q000378:metabolism* / Q000503:physiopathology; D002784:Cholesterol / Q000032:analysis; D004268:DNA-Binding Proteins; D004848:Epithelium; D006801:Humans; D012333:RNA, Messenger / Q000096:biosynthesis; D011956:Receptors, Cell Surface / Q000096:biosynthesis*; D025521:Tumor Suppressor Proteins
D000373:Agglutinins; D002135:Calcium-Binding Proteins; C108174:DMBT1 protein, human; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D025521:Tumor Suppressor Proteins; D002784:Cholesterol