title: High cell density cultivation of Brevibacterium linens and formation of proteinases and lipase.
abstract: Brevibacterium linens forms hydrolytic enzymes which can be used to accelerate the ripening of cheese without causing bitterness. B. linens ATCC 9172 was grown to a high cell density (50 g dry wt l-1 after 60 h) in a mineral medium containing lactic acid, soy-peptone and ammonium sulphate by applying a continuous feed of nutrients. The maximal activities of L-leucine aminopeptidase and cell-associated proteinase were 286 U l-1 and 202 U l-1, respectively. The cell-associated lipolytic activity exhibited a strong and sudden increase at 46 h, resulting in a maximum of 9.5 U g-1 dry wt; thus the volumetric productivity of proteolytic and lipolytic activity was 4220 U l-1 h-1 and 7.3 U l-1 h-1, respectively.
authors.affiliation: Institute of Chemical Engineering, Vienna University of Technology, Getreidemarkt 9/166, A-1060 Vienna, Austria. bernhard.adamitsch@tuwien.ac.at
authors.forename: Bernhard F
authors.initials: BF
authors.lastname: Adamitsch
authors.forename: Ferdinand
authors.initials: F
authors.lastname: Karner
authors.forename: Werner A
authors.initials: WA
authors.lastname: Hampel
mesh_terms: D000645:Ammonium Sulfate / Q000494:pharmacology; D001951:Brevibacterium / Q000145:classification / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development*; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D010450:Endopeptidases / Q000096:biosynthesis; D004789:Enzyme Activation; D019344:Lactic Acid / Q000494:pharmacology; D007931:Leucyl Aminopeptidase / Q000096:biosynthesis; D008049:Lipase / Q000096:biosynthesis*; D010461:Peptones / Q000494:pharmacology; D011786:Quality Control; D030262:Soybean Proteins / Q000494:pharmacology; D013045:Species Specificity
chemical_list: D003470:Culture Media; D010461:Peptones; D030262:Soybean Proteins; D019344:Lactic Acid; D008049:Lipase; D010450:Endopeptidases; D007931:Leucyl Aminopeptidase; D000645:Ammonium Sulfate