[Experimental retinal vein occlusion induced by photodynamic approach].
OBJECTIVE
To develop a new photochemical method of experimental retinal vein occlusion and investigate the morphologic and histologic changes in the retina.


METHODS
After intravenous injection of rose Bengal, the vessels next to the disc of miniature pigs in the experiment group (n = 15) were exposed to an endo-illuminator for 15 minutes. As a control group, the vessels next to the disc of each pig were exposed to the endo-illuminator without rose Bengal injection. After complete vascular occlusion, the eyes were observed at following times: one hour, and 1, 3, 7, 14, 21, and 28 days. After the 28th day, the eyes were enucleated and prepared for light and electron microscope examination.


RESULTS
Histopathologic features were consistent with changes of retinal vein occlusion and formation of retinal vein thrombi was reliable.


CONCLUSIONS
Photodynamic method combined with endo-illuminator was a simple, reliable and definitive experimental technique to produce retinal vein occlusion.
Zhang
Xiao-li
XL
Department of Ophthalmogy General Hospital of PLA, Beijing 100853, China.
Hu
Yun-tao
YT
Ma
Zhi-zhong
ZZ
Wang
Chang-guan
CG
Han
Su-yi
SY
Yang
Bing-jian
BJ
Huang
Li-jie
LJ
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D008297:Male; D010778:Photochemotherapy / Q000009:adverse effects; D012170:Retinal Vein Occlusion / Q000209:etiology / Q000473:pathology*; D012395:Rose Bengal / Q000009:adverse effects; D013552:Swine; D013556:Swine, Miniature
D012395:Rose Bengal