title: Human dendritic cell subsets in NOD/SCID mice engrafted with CD34+ hematopoietic progenitors.
abstract: Distinct human dendritic cell (DC) subsets differentially control immunity. Thus, insights into their in vivo functions are important to understand the launching and modulation of immune responses. We show that nonobese diabetic/LtSz-scid/scid (NOD/SCID) mice engrafted with human CD34+ hematopoietic progenitors develop human myeloid and plasmacytoid DCs. The skin displays immature DCs expressing Langerin, while other tissues display interstitial DCs. Myeloid DCs from these mice induce proliferation of allogeneic CD4 T cells in vitro, and bone marrow human cells containing plasmacytoid DCs release interferon-alpha (IFN-alpha) upon influenza virus exposure. Injection of influenza virus into reconstituted mice triggers IFN-alpha release and maturation of mDCs. Thus, these mice may provide a model to study the pathophysiology of human DC subsets.
authors.affiliation: Baylor Institute for Immunology Research, 3434 Live Oak, Dallas, TX 75204, USA. karolinp@baylorhealth.edu
authors.forename: A Karolina
authors.initials: AK
authors.lastname: Palucka
authors.forename: Joel
authors.initials: J
authors.lastname: Gatlin
authors.forename: Jean Philippe
authors.initials: JP
authors.lastname: Blanck
authors.forename: Michael W
authors.initials: MW
authors.lastname: Melkus
authors.forename: Sandra
authors.initials: S
authors.lastname: Clayton
authors.forename: Hideki
authors.initials: H
authors.lastname: Ueno
authors.forename: Elizabeth T
authors.initials: ET
authors.lastname: Kraus
authors.forename: Petra
authors.initials: P
authors.lastname: Cravens
authors.forename: Lynda
authors.initials: L
authors.lastname: Bennett
authors.forename: Angela
authors.initials: A
authors.lastname: Padgett-Thomas
authors.forename: Florentina
authors.initials: F
authors.lastname: Marches
authors.forename: Miguel
authors.initials: M
authors.lastname: Islas-Ohlmayer
authors.forename: J Victor
authors.initials: JV
authors.lastname: Garcia
authors.forename: Jacques
authors.initials: J
authors.lastname: Banchereau
mesh_terms: D000818:Animals; D018952:Antigens, CD34; D001773:Blood Cells; D001854:Bone Marrow Cells; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D003713:Dendritic Cells / Q000145:classification / Q000166:cytology* / Q000276:immunology; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D016898:Interferon-alpha / Q000378:metabolism; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D016513:Mice, SCID; D009975:Orthomyxoviridae / Q000276:immunology; D014183:Transplantation, Heterologous
chemical_list: D018952:Antigens, CD34; D016898:Interferon-alpha