Tissue engineering of arteries by directed remodeling of intact arterial segments.
Traditional approaches to generating tissue-engineered arteries in vitro rely on expansion of cells in culture to seed appropriate scaffolds. In most envisioned applications, small autologous blood vessels would be harvested and used as a source for these cells. We propose that small autologous arteries, not the cells derived from them, may be an attractive starting point for engineered arteries. This approach capitalizes on the ability of intact arteries to grow and remodel in response to chronic changes in their mechanical environment. Carotid arteries from juvenile (approximately 30-kg) pigs were stretched longitudinally in an ex vivo perfusion system over 9 days. This resulted in a 40% increase in artery length at physiological longitudinal stress and a 20 +/- 3% increase when unstressed. Control arteries were perfused for 9 days ex vivo at their physiological loaded length. Control and elongated arteries displayed native appearance (macroscopic and histological), excellent viability (cellularity and mitochondrial activity), normal vasoactivity, and similar mechanical properties (ultimate stress and ultimate strain) as compared with freshly harvested arteries. Growth, as opposed to just redistribution of existing mass, contributed to elongation as evidenced by an increase in artery weight. Results on elongation of arteries from neonatal and adolescent pigs are also presented and discussed.
Clerin
Valerie
V
Wyeth Research, Cambridge, Massachusetts, USA.
Nichol
Jason W
JW
Petko
Matus
M
Myung
Richard J
RJ
Gaynor
J William
JW
Gooch
Keith J
KJ
D000818:Animals; D001158:Arteries; D002339:Carotid Arteries; D010477:Perfusion / Q000295:instrumentation / Q000379:methods; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*