title: Maleic anhydride copolymers--a versatile platform for molecular biosurface engineering.
abstract: A platform of thin polymer coatings was introduced for the functional modulation of immobilized bioactive molecules at solid/liquid interfaces. The approach is based on covalently attached alternating maleic acid anhydride copolymers with a variety of comonomers and extended through conversion of the anhydride moieties by hydrolysis, reaction with functional amines, and other conversions of the anhydride moieties. We demonstrate that these options permit control of the physicochemical constraints for bioactive molecules immobilized at interfaces to influence important performance characteristics of biofunctionalized materials for medical devices and molecular diagnostics. Examples concern the impact of the substrate-anchorage of fibronectin on the formation of cell-matrix adhesions, the orientation of endothelial cells according to lateral anti-adhesive micropatterns using grafted poly(ethylene oxide), and the spacer-dependent activity of immobilized synthetic thrombin inhibitors.
authors.affiliation: Institute of Polymer Research Dresden and The Max Bergmann Center of Biomaterials Dresden, Hohe Str. 6, 01069 Dresden, Germany.
authors.forename: Tilo
authors.initials: T
authors.lastname: Pompe
authors.forename: Stefan
authors.initials: S
authors.lastname: Zschoche
authors.forename: Nicole
authors.initials: N
authors.lastname: Herold
authors.forename: Katrin
authors.initials: K
authors.lastname: Salchert
authors.forename: Marie-Francoise
authors.initials: MF
authors.lastname: Gouzy
authors.forename: Claudia
authors.initials: C
authors.lastname: Sperling
authors.forename: Carsten
authors.initials: C
authors.lastname: Werner
mesh_terms: D000588:Amines / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry; D001698:Biomedical Engineering / Q000379:methods*; D002448:Cell Adhesion / Q000187:drug effects; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects; D005353:Fibronectins / Q000737:chemistry; D006801:Humans; D006868:Hydrolysis; D008299:Maleic Anhydrides / Q000737:chemistry*; D015394:Molecular Structure; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D000588:Amines; D001672:Biocompatible Materials; D005353:Fibronectins; D008299:Maleic Anhydrides; D011108:Polymers; C016683:polymaleic anhydride; D011092:Polyethylene Glycols