DNA microarray analysis of in vivo progression mechanism of heart failure.
Dahl salt-sensitive rats are genetically hypersensitive to sodium intake. When fed a high sodium diet, they develop systemic hypertension, followed by cardiac hypertrophy and finally heart failure within a few months. Therefore, Dahl rats represent a good model with which to study how heart failure is developed in vivo. By using DNA microarray, we here monitored the transcriptome of >8000 genes in the left ventricular muscles of Dahl rats during the course of cardiovascular damage. Expression of the atrial natriuretic peptide gene was, for instance, induced in myocytes by sodium overload and further enhanced even at the heart failure stage. Interestingly, expression of the gene for the D-binding protein, an apoptotic-related transcriptional factor, became decreased upon the transition to heart failure. To our best knowledge, this is the first report to describe the transcriptome of cardiac myocytes during the disease progression of heart failure.
Ueno
Shuichi
S
Division of Functional Genomics, Jichi Medical School, 3311-1 Yakushiji, Kawachi-gun, Tochigi 329-0498, Japan.
Ohki
Ruri
R
Hashimoto
Toru
T
Takizawa
Toshihiro
T
Takeuchi
Koichi
K
Yamashita
Yoshihiro
Y
Ota
Jun
J
Choi
Young Lim
YL
Wada
Tomoaki
T
Koinuma
Koji
K
Yamamoto
Keiji
K
Ikeda
Uichi
U
Shimada
Kazuyuki
K
Mano
Hiroyuki
H
D000818:Animals; D006332:Cardiomegaly / Q000209:etiology / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002478:Cells, Cultured; D018450:Disease Progression; D015536:Down-Regulation; D020869:Gene Expression Profiling; D006333:Heart Failure / Q000209:etiology* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D032383:Myocytes, Cardiac / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D020303:Rats, Inbred Dahl; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic