Bioreactor cultivation of Escherichia coli for production of recombinant penicillin G amidase from Alcaligenes faecalis.
The penicillin G amidase (PGA) from Alcaligenes faecalis, which has interesting properties for use in combinatorial biochemistry, was produced by recombinant expression in Escherichia coli. The corresponding gene was cloned into a multicopy vector under the strict regulatory control of the rhamnose inducible promoter. Cells were grown in a synthetic minimal medium in a bioreactor (5 l working vol.), and production of PGA was induced by repeated addition of the inducer rhamnose, that served also as a carbon source. The fermentation yield was about 4500 units PGA activity per liter of culture medium.
Deak
Peter M
PM
Lehrstuhl für Biotechnologie, Institut für Lebensmitteltechnologie, Universität Hohenheim, Emil-Wolff-Strasse 14, 70599 Stuttgart, Germany.
Lutz-Wahl
Sabine
S
Bothe
Harald
H
Fischer
Lutz
L
D000421:Alcaligenes / Q000145:classification / Q000201:enzymology* / Q000235:genetics*; D019149:Bioreactors / Q000382:microbiology*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D010399:Penicillin Amidase / Q000096:biosynthesis*; D011786:Quality Control; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D012210:Rhamnose / Q000378:metabolism*; D013045:Species Specificity; D014158:Transcription, Genetic
D011994:Recombinant Proteins; D010399:Penicillin Amidase; D012210:Rhamnose