title: [Polymorphism of CXCR4 coding region of human immunodeficiency virus-1 in Chinese Han people].
abstract: OBJECTIVE
  To study the polymorphism of human immunodeficiency virus (HIV)-1 coreceptor CXCR4 in Chinese Han ethnic group for AIDS prevention and treatment.
  
  
  METHODS
  Totally 48 individuals were enrolled into the study. CXCR4 (cDNA No-AF147204) was cloned by PCR amplification using 2 pairs of primers, then sequenced using sequencing primers. The results of the same sequencing primers were analyzed by DNAstar software to find and identify single nucleotide polymorphism (SNP) sites.
  
  
  RESULTS
  Totally 7 SNPs were found in the coding region of CXCR4, among them 3 were synonymous mutation (C-->T at loci 129, 426 and 968), 3 were missense mutation (C-->T at locus 38, A-->T at locus 90, and A-->C at locus 712) and 1 was stop mutation (C-->T at 106, which converted the codon for glutamic acid into stop codon).
  
  
  CONCLUSIONS
  The polymorphism of CXCR4 coding region in Chinese Han is probably different from that of the other ethnic groups. Six of the 7 SNPs were discovered for the first time. Their influences on AIDS progression are worthy of studying.
authors.affiliation: The No. 302 Hospital of PLA, Beijing 100039, China.
authors.forename: Ming-xu
authors.initials: MX
authors.lastname: Liu
authors.forename: Fu-sheng
authors.initials: FS
authors.lastname: Wang
authors.forename: Wei-guo
authors.initials: WG
authors.lastname: Hong
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Jin
authors.forename: Zhou-yun
authors.initials: ZY
authors.lastname: Lei
authors.forename: Jing
authors.initials: J
authors.lastname: Hou
mesh_terms: D000328:Adult; D044466:Asian People; D001483:Base Sequence; D002681:China / Q000208:ethnology; D005260:Female; D005787:Gene Frequency; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D017354:Point Mutation; D020641:Polymorphism, Single Nucleotide; D019718:Receptors, CXCR4 / Q000235:genetics*
chemical_list: D019718:Receptors, CXCR4