Comparison of glucan detection and galactomannan enzyme immunoassay in gastrointestinal and systemic murine candidiasis.
Mouse models of systemic and gastrointestinal infection with the yeast Candida albicans were used to investigate the ability of a commercial mannan antigen enzyme immunoassay and a commercial (1-->3) beta-D-glucan limulus assay to detect systemic infection and to differentiate between colonization and infection. Both assays were positive in all i.v. infected mice and negative in all uninfected control mice. In gastrointestinal infection both tests were positive whenever organ cultures were positive. In colonized mice with no detectable dissemination, there were mostly negative results with the glucan assay whereas the mannan assay was positive or intermediate in all colonized mice. Therefore, in the mouse model used, glucan detection appeared to be superior for differentiation between colonization and dissemination.
Nichterlein
Thomas
T
Institut für Medizinische Mikrobiologie und Hygiene, Klinikum Mannheim, D-68167 Mannheim, Germany.
Buchheidt
Dieter
D
Hein
Andreas
A
Becker
Klaus-Peter
KP
Mosbach
Korinna
K
Kretschmar
Marianne
M
D000818:Animals; D002176:Candida albicans / Q000302:isolation & purification*; D002177:Candidiasis / Q000175:diagnosis* / Q000201:enzymology; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D016469:Fungemia / Q000175:diagnosis* / Q000209:etiology; D005690:Galactose / Q000031:analogs & derivatives; D005767:Gastrointestinal Diseases / Q000175:diagnosis* / Q000201:enzymology; D005936:Glucans / Q000032:analysis*; D016014:Linear Models; D008351:Mannans / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D011336:Probability; D012680:Sensitivity and Specificity
D005936:Glucans; D008351:Mannans; C012990:galactomannan; D005690:Galactose