Effects of caffeine and chlorogenic acid on propidium iodide accessibility to DNA: consequences on genome size evaluation in coffee tree.
Estimates of genome size using flow cytometry can be biased by the presence of cytosolic compounds, leading to pseudo-intraspecific variation in genome size. Two important compounds present in coffee trees-caffeine and chlorogenic acid-modify accessibility of the dye propidium iodide to Petunia DNA, a species used as internal standard in our genome size evaluation. These compounds could be responsible for intraspecific variation in genome size since their contents vary between trees. They could also be implicated in environmental variations in genome size, such as those revealed when comparing the results of evaluations carried out on different dates on several genotypes.
Noirot
M
M
IRD BP5045, 34032 Montpellier Cedex, France. noirot@mpl.ird.fr
Barre
P
P
Duperray
C
C
Louarn
J
J
Hamon
S
S
D002110:Caffeine; D002726:Chlorogenic Acid; D040503:Coffea / Q000235:genetics*; D004396:Coloring Agents / Q000032:analysis / Q000378:metabolism; D018744:DNA, Plant / Q000032:analysis / Q000378:metabolism*; D005434:Flow Cytometry / Q000379:methods*; D018745:Genome, Plant; D007364:Intercalating Agents / Q000032:analysis / Q000378:metabolism*; D011419:Propidium / Q000032:analysis / Q000378:metabolism*; D012015:Reference Standards
D004396:Coloring Agents; D018744:DNA, Plant; D007364:Intercalating Agents; D002726:Chlorogenic Acid; D011419:Propidium; D002110:Caffeine