title: Effects of carbon dioxide and nitrogen on adhesive growth and expressions of E-cadherin and VEGF of human colon cancer cell CCL-228.
abstract: OBJECTIVE
  To study the effects of carbon dioxide on the metastatic capability of cancer cells, and to compare them with that of nitrogen.
  
  
  METHODS
  The colon cancer cell CCL-228 was treated with 100 % carbon dioxide or nitrogen at different time points and then cultured under normal condition. Twelve hours after the treatment, the survival rates of suspension cells and the expressions of e-cadherin and VEGF were examined.
  
  
  RESULTS
  After 60 min of carbon dioxide and longer time of nitrogen treatment, the suspended cells increased and the expression of e-cadherin decreased while the expression of VEGF was enhanced significantly. And the effects of nitrogen were similar to, but weaker than, those of carbon dioxide.
  
  
  CONCLUSIONS
  Carbon dioxide may improve the metastatic capability of cancer cells and its effects are significantly stronger than that of nitrogen. A sequential use of carbon dioxide and nitrogen in pneumoperitoneum may take the advantage of both gases.
authors.affiliation: General Surgery Department, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China. caikailin@yahoo.com.cn
authors.forename: Kai-Lin
authors.initials: KL
authors.lastname: Cai
authors.forename: Guo-Bing
authors.initials: GB
authors.lastname: Wang
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Xiong
mesh_terms: D015820:Cadherins / Q000096:biosynthesis*; D002245:Carbon Dioxide / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D003110:Colonic Neoplasms; D006801:Humans; D009584:Nitrogen / Q000494:pharmacology*; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis*
chemical_list: D015820:Cadherins; D042461:Vascular Endothelial Growth Factor A; D002245:Carbon Dioxide; D009584:Nitrogen