Simultaneous determination of spirapril and spiraprilat in plasma by capillary gas chromatography-mass spectrometry.
A specific, sensitive and precise method for the simultaneous determination of spirapril (CAS 94841-17-5) and spiraprilat (CAS 83602-05-5) in human plasma is described. The method involves the use of enalapril as internal standard, solid-phase extraction, derivatization and capillary gas chromatography with mass sensitive detection. The working range is from 2.5 to 500 micrograms/l for spirapril and spiraprilat, respectively. Data demonstrating the precision and accuracy of the analytical method are given. Moreover, data concerning freeze-thaw stability, long-term stability of frozen samples, short-term stability of thawed samples, and stability of the extracts in the autosampler are given.
Schürer
Michael
M
LAFAA Laboratory for Contract Research in Clinical Pharmacology and Biopharmaceutical Analytics GmbH, Bad Schwartau Germany. lafaa_gmbh@t-onbline.de
Amschler
Stefan
S
Schulz
Hans-Ulrich
HU
Schäfer
Harald F
HF
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000097:blood*; D004656:Enalapril / Q000031:analogs & derivatives* / Q000097:blood*; D005615:Freezing; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007202:Indicators and Reagents; D012015:Reference Standards; D015203:Reproducibility of Results; D012996:Solutions
D000806:Angiotensin-Converting Enzyme Inhibitors; D007202:Indicators and Reagents; D012996:Solutions; D004656:Enalapril; C052555:spirapril; C076884:spiraprilat