title: Novel approach for SNP genotyping based on site-selective RNA scission.
abstract: Novel genotyping method for single nucleotide polymorphisms (SNPs), based on site-selective RNA scission, has been developed. A substrate RNA is activated at two sites by complementary acridine-modified DNA having two acridine residues, and is site-selectively cleaved by metal ion catalyst to produce short RNA fragment containing the SNP site. Genotype of the substrate is accurately and easily determined by mass analysis of the fragment.
authors.affiliation: Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
authors.forename: Akinori
authors.initials: A
authors.lastname: Kuzuya
authors.forename: Ryo
authors.initials: R
authors.lastname: Mizoguchi
authors.forename: Fumi
authors.initials: F
authors.lastname: Morisawa
authors.forename: Makoto
authors.initials: M
authors.lastname: Komiyama
mesh_terms: D001483:Base Sequence; D005838:Genotype; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D012313:RNA / Q000235:genetics*
chemical_list: D012313:RNA