Evaluation of arbitrarily primed PCR for typing of Desulfovibrio desulfuricans strains.
Arbitrarily primed polymerase chain reaction (AP-PCR) method was applied to the differentiation of 15 (soil and intestinal) Desulfovibrio desulfuricans strains. The primer M 13, which is a core sequence of phage M 13, was found to be appropriate for the differentiation of isolates of this species. The analysis revealed characteristic band patterns for all of the examined strains of which two soil strains (DV-7 and DV-8) showed identical DNA fingerprints. According to Jaccard's coefficient, the soil bacterial group as well as intestinal bacterial group formed two different clusters. Furthermore, the soil strains showed greater variability than the intestinal isolates. Based on the AP-PCR fingerprints D. desulfuricans strains were differentiated depending on their origin. This study demonstrates that the typing method AP-PCR can be useful in epidemiologic investigations as a rapid and valuable tool for differentiation of the strains of D. desulfuricans species.
Dzierzewicz
Zofia
Z
Department of Molecular Biology, Biochemistry and Biopharmacy, Medical University of Silesia, Narcyzów 1, 41-200 Sosnowiec, Poland. zofiadz@slam.katowice.pl
Szczerba
Joanna
J
Weglarz
Ludmiła
L
Komarska-Szostak
Anna
A
Wilczok
Tadeusz
T
D015373:Bacterial Typing Techniques; D002432:Cecum / Q000382:microbiology; D016172:DNA Fingerprinting; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D003901:Desulfovibrio / Q000145:classification* / Q000235:genetics; D005243:Feces / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction; D012988:Soil Microbiology
D017931:DNA Primers; D004269:DNA, Bacterial