[The relationship between tumor suppressor genes p14ARF and p53 expression and biological behavior of pancreatic carcinoma].
OBJECTIVE
To investigate the relationship between tumor suppressor genes p14(ARF) and p53 expression and the biological behavior of pancreatic carcinoma.


METHODS
Modified Envision method of immunohistochemistry was used on 42 specimens of pancreatic adenocarcinoma and 10 normal specimens of normal pancreas resected during operation to examine the expression of the gene p14(ARF). and ABC immunohistochemical technique was used to examine the expression of p53.


RESULTS
The positive rates of p14(ARF) in of normal pancreatic tissues and pancreatic carcinoma were 90% and 35.7% respectively (P < 0.01). The positive rates of p53 in tissues of normal pancreatic and pancreatic carcinoma were 0 and 42.5% (P < 0.05) respectively. Significant correlation was detected between the expression of p14(AR) and p53 and the diameter of tumor, the rate of lymph node metastasis, pathological grade, and clinic stage in pancreatic carcinoma. No correlation was found between the expression of p14(ARF) and p53 and the tumor infiltration in pancreatic carcinoma.


CONCLUSIONS
The tumor suppressor gene p14(ARF) and p53 are closely related to the occurrence and development of pancreatic carcinoma. It is possible to treat pancreatic carcinoma by gene intervention.
Chen
Xiao-lei
XL
Center for Pancreatic Cancer, Huashan Hospital, Fudan University, Shanghai 200040, China.
Zhang
Qun-hua
QH
Ni
Quan-xing
QX
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000473:pathology; D025803:Tumor Suppressor Protein p14ARF / Q000032:analysis* / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*
D025803:Tumor Suppressor Protein p14ARF; D016159:Tumor Suppressor Protein p53