Peptide and amino acid transporters are differentially regulated during seed development and germination in faba bean.
Two peptide transporter (PTR) homologs have been isolated from developing seeds of faba bean (Vicia faba). VfPTR1 was shown to be a functional peptide transporter through complementation of a yeast mutant. Expression patterns of VfPTR1 and VfPTR2 as well as of the amino acid permease VfAAP1 (Miranda et al., 2001) were compared throughout seed development and germination. In developing seeds, the highest levels of VfPTR1 transcripts were reached during midcotyledon development, whereas VfAAP1 transcripts were most abundant during early cotyledon development, before the appearance of storage protein gene transcripts, and were detectable until late cotyledon development. During early germination, VfPTR1 mRNA appeared first in cotyledons and later, during seedling growth, also in axes and roots. Expression of VfPTR2 and VfAAP1 was delayed compared with VfPTR1, and was restricted to the nascent organs of the seedlings. Localization of VfPTR1 transcripts showed that this PTR is temporally and spatially regulated during cotyledon development. In germinating seeds, VfPTR1 mRNA was localized in root hairs and root epidermal cells, suggesting a role in nutrient uptake from the soil. In seedling roots, VfPTR1 was repressed by a dipeptide and by an amino acid, whereas nitrate was without influence.
Miranda
Manoela
M
Institut für Pflanzengenetik und Kulturpflanzenforschung, Gatersleben, D-06466 Germany. miranda@uvic.ca
Borisjuk
Ljudmilla
L
Tewes
Annegret
A
Dietrich
Daniela
D
Rentsch
Doris
D
Weber
Hans
H
Wobus
Ulrich
U
D026905:Amino Acid Transport Systems / Q000235:genetics*; D000596:Amino Acids / Q000378:metabolism; D001693:Biological Transport, Active; D003001:Cloning, Molecular; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D018525:Germination; D026901:Membrane Transport Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D010802:Phylogeny; D018517:Plant Roots / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000235:genetics* / Q000254:growth & development; D031307:Vicia faba / Q000235:genetics* / Q000254:growth & development*; D015003:Yeasts / Q000235:genetics / Q000378:metabolism
D026905:Amino Acid Transport Systems; D000596:Amino Acids; D026901:Membrane Transport Proteins; D010455:Peptides; D012333:RNA, Messenger; D018749:RNA, Plant; C043912:peptide permease; D009584:Nitrogen