title: TGF-beta1 induces accumulation of dendritic cells in the odontoblast layer.
abstract: TGF-beta1 released from dentin degraded by bacterial or iatrogenic agents is suspected to influence dental pulp response, including the modulation of cell migration. To determine the consequences of TGF-beta1 action on pulp immune cells, we analyzed, by immunohistochemistry, the effect of transdentinally diffusing TGF-beta1 on their localization in a human tooth slice culture model. TGF-beta1 induced an accumulation of HLA-DR-positive cells in both odontoblast and subodontoblast layers of the stimulated zone. Together with HLA-DR, these cells co-expressed Factor XIIIa and CD68, two features of immature antigen-presenting dendritic cells (DC), as well as the TGF-beta1 specific receptor TbetaRII. In contrast, no effect could be detected on the localization of either mature DC-LAMP-positive DC or of T- and B-lymphocytes. Analysis of these data suggests that TGF-beta1 released from dentin degraded by bacterial or iatrogenic agents could be involved in the immune response of the dental pulp resulting from tooth injury.
authors.affiliation: Laboratory of Development of Dental Tissues, EA MENRT 1892, IFR 62, Faculty of Odontology, Lyon 1 University, G. Paradin Str., 69372 Lyon Cedex 08, France. farges@laennec.univ-lyon1.fr
authors.forename: J-C
authors.initials: JC
authors.lastname: Farges
authors.forename: A
authors.initials: A
authors.lastname: Romeas
authors.forename: M
authors.initials: M
authors.lastname: Melin
authors.forename: J-J
authors.initials: JJ
authors.lastname: Pin
authors.forename: S
authors.initials: S
authors.lastname: Lebecque
authors.forename: M
authors.initials: M
authors.lastname: Lucchini
authors.forename: F
authors.initials: F
authors.lastname: Bleicher
authors.forename: H
authors.initials: H
authors.lastname: Magloire
mesh_terms: D000293:Adolescent; D000938:Antigen-Presenting Cells / Q000187:drug effects; D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D001402:B-Lymphocytes / Q000187:drug effects; D002452:Cell Count; D002465:Cell Movement / Q000187:drug effects; D046508:Culture Techniques; D003713:Dendritic Cells / Q000187:drug effects*; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects; D026122:Factor XIIIa / Q000032:analysis; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D051907:Lysosomal Membrane Proteins; D009804:Odontoblasts / Q000187:drug effects*; D018125:Receptors, Transforming Growth Factor beta / Q000032:analysis; D013601:T-Lymphocytes / Q000187:drug effects; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D026122:Factor XIIIa; D006684:HLA-DR Antigens; D051907:Lysosomal Membrane Proteins; D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; C067980:CD68 antigen, human; D000097382:CD68 Molecule; C508960:TGFB1 protein, human