Photo-regulation of transcription by RNA polymerase with azobenzene-tethered promoter.
Photo-regulation of transcription reaction by T7-RNA polymerase was investigated by introducing an azobenzene residue into the non-template strand of T7-promoter. It was found that transcription was strongly interfered under dark or visible light irradiation, whereas it efficiently proceeded after UV irradiation. With this photo-responsive promoter, on-off switching of transcription by light irradiation was achieved. This strategy was also effective for the photo-regulation of SP6-RNA polymerase.
Asanuma
Hiroyuki
H
Research Center of Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
Liu
Mingzhe
M
Tamaru
Daisuke
D
Liang
Xingguo
X
Komiyama
Makoto
M
D001483:Base Sequence; D004247:DNA / Q000737:chemistry; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D014466:Ultraviolet Rays; D014764:Viral Proteins
D014764:Viral Proteins; D004247:DNA; C070852:RNA polymerase SP6; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases