[Protective effect against Schistosoma japonicum of recombinant fusion protein SjGST-32 in mice].
OBJECTIVE
To explore the optimal immune doses of recombinant antigen rSjGST-32, with QuilA as adjuvant.


METHODS
BALB/c mice were immunized with doses of 50, 100 and 200 micrograms rSjGST-32/mouse plus 50 micrograms QuilA adjuvant. QuilA and PBS were used as controls. Levels of specific antibodies were detected by ELISA. The mice were challenged 4 weeks after last vaccination. Worms and eggs collected from the livers of mice were counted 45 days after challenge.


RESULTS
As compared with the control groups, the worm reduction rate in the 50, 100 and 200 micrograms experiment groups was 38.1%, 47.8% and 48.8%, respectively. The reduction rate of liver eggs per gram (LEPG) was 39.1%, 53.5% and 53.6%, respectively, and the reduction rate of the liver eggs per female (LEPF) was 22.5%, 22.8% and 21.8%, respectively. The specific antibody titers in sera reached 1:51,200, 1:102,400 and 1:102,400, respectively before challenge.


CONCLUSIONS
The results show that for vaccinating BALB/c mice, the optimal antigen dose is 100 micrograms of recombinant rSjGST-32 plus QuilA adjuvant.
Tian
Ming-li
ML
Department of Parasitology, Xiangya Medical College, Central Southern University, Changsha 410078.
Cai
Chun
C
Yi
Xin-yuan
XY
Zeng
Xian-fang
XF
Zhang
Shun-ke
SK
D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D003546:Cysteine Endopeptidases / Q000008:administration & dosage / Q000276:immunology*; D005260:Female; D005982:Glutathione Transferase / Q000008:administration & dosage / Q000276:immunology*; D015801:Helminth Proteins / Q000008:administration & dosage / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D062605:Quillaja Saponins; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000276:immunology; D012503:Saponins / Q000008:administration & dosage; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000276:immunology / Q000517:prevention & control*
D000276:Adjuvants, Immunologic; D000909:Antibodies, Helminth; D015801:Helminth Proteins; D062605:Quillaja Saponins; D011993:Recombinant Fusion Proteins; D012503:Saponins; C046386:Quil A; D005982:Glutathione Transferase; D003546:Cysteine Endopeptidases