Unexpected high testis-specific transcriptional activity of the cyclin T1 promoter in transgenic mice.
The ubiquitously expressed cyclin T1 gene encodes for a protein involved in human immunodeficiency virus type 1 (HIV-1) transcription activation. The goat gene was recently shown to share an expression pattern similar to that of its endogenous counterpart when incorporated into mice using a BAC insert. To assess if its promoter could target ubiquitous expression of the bovine Prnp in transgenic mice, two constructs carrying either 1 or 30 kb of cyclin T1 5'-flanking sequences were built and microinjected. Both constructs resulted in the unexpected high male germ cell-specific expression of the prion protein. These data re-question the suspected location of the cyclin T1 gene regulatory elements.
Mata
X
X
Laboratoire de Génétique Biochimique et de Cytogénétique, INRA, 78352 Cedex, Jouy-en-Josas, France. mata@jouy.inra.fr
Besnard
N
N
Le Roux
K
K
Tilly
G
G
Andréoletti
O
O
Hudrisier
M
M
Costa Da Silva
J
J
Laude
H
H
Vilotte
J L
JL
D024506:5' Flanking Region; D000818:Animals; D002417:Cattle; D056906:Cyclin T; D016213:Cyclins / Q000235:genetics* / Q000378:metabolism; D005822:Genetic Vectors; D005854:Germ Cells / Q000378:metabolism; D006041:Goats; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D008845:Microinjections; D011328:Prions / Q000096:biosynthesis / Q000235:genetics; D011401:Promoter Regions, Genetic; D013737:Testis / Q000166:cytology / Q000378:metabolism*; D014018:Tissue Distribution; D014158:Transcription, Genetic
C503684:CCNT1 protein, human; C503685:Ccnt1 protein, mouse; D056906:Cyclin T; D016213:Cyclins; D011328:Prions