Cation channels, cell volume and the death of an erythrocyte.
Similar to a variety of nucleated cells, human erythrocytes activate a non-selective cation channel upon osmotic cell shrinkage. Further stimuli of channel activation include oxidative stress, energy depletion and extracellular removal of Cl-. The channel is permeable to Ca2+ and opening of the channel increases cytosolic [Ca2+]. Intriguing evidence points to a role of this channel in the elimination of erythrocytes by apoptosis. Ca2+ entering through the cation channel stimulates a scramblase, leading to breakdown of cell membrane phosphatidylserine asymmetry, and stimulates Ca(2+)-sensitive K+ channels, thus leading to KCl loss and (further) cell shrinkage. The breakdown of phosphatidylserine asymmetry is evidenced by annexin binding, a typical feature of apoptotic cells. The effects of osmotic shock, oxidative stress and energy depletion on annexin binding are mimicked by the Ca2+ ionophore ionomycin (1 microM) and blunted in the nominal absence of extracellular Ca2+. Nevertheless, the residual annexin binding points to additional mechanisms involved in the triggering of the scramblase. The exposure of phosphatidylserine at the extracellular face of the cell membrane stimulates phagocytes to engulf the apoptotic erythrocytes. Thus, sustained activation of the cation channels eventually leads to clearance of affected erythrocytes from peripheral blood. Susceptibility to annexin binding is enhanced in several genetic disorders affecting erythrocyte function, such as thalassaemia, sickle-cell disease and glucose-6-phosphate dehydrogenase deficiency. The enhanced vulnerability presumably contributes to the shortened life span of the affected erythrocytes. Beyond their role in the limitation of erythrocyte survival, cation channels may contribute to the triggering of apoptosis in nucleated cells exposed to osmotic shock and/or oxidative stress.
Lang
Florian
F
Physiologisches Institut der Universität Tübingen, Gmelinstrasse 5, 72076 Tübingen, Germany. florian.lang@uni-tuebingen.de
Lang
Karl S
KS
Wieder
Thomas
T
Myssina
Svetlana
S
Birka
Christina
C
Lang
Philipp A
PA
Kaiser
Stephanie
S
Kempe
Daniela
D
Duranton
Christophe
C
Huber
Stephan M
SM
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002412:Cations / Q000378:metabolism*; D016922:Cellular Senescence / Q000502:physiology; D004912:Erythrocytes / Q000166:cytology* / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D007473:Ion Channels / Q000097:blood*
D002412:Cations; D007473:Ion Channels