Utilization for lactic acid production with a new acid hydrolysis of ram horn waste.
The use of ram horn hydrolysate (RHH) as a substrate for lactic acid production was investigated using Lactobacillus casei. For this purpose, first RHH was produced. Ram horns were hydrolyzed by treating with acids (6N-H2SO4 and 6N-HCl) and neutralizing the solutions. The amounts of protein, nitrogen, ash, some minerals, total sugars, total lipids and amino acids of the RHH were determined. The effect of different concentrations (1-10% v/v) of RHH on the production of biomass, lactic acid concentration and sugar consumption was investigated, and a concentration of 6% RHH was found to be optimal. The content of lactic acid in the culture broth containing 6% RHH (44 g x l(-1)) for 26 h was 22% higher than that of the control culture broth (36 g x l(-1)). From this result, RHH was demonstrated to be a suitable supplement for lactic acid production, a use that would resolve a local environmental problem.
Kurbanoglu
Esabi Basaran
EB
Science and Letters Faculty, Department of Biology, Atatürk University, 25240 Erzurum, Turkey. ekurbanoglu@yahoo.com
Kurbanoglu
Namudar Izzet
NI
D000818:Animals; D003470:Culture Media; D005285:Fermentation; D006851:Hydrochloric Acid; D006868:Hydrolysis; D066298:In Vitro Techniques; D007220:Industrial Waste / Q000032:analysis; D019344:Lactic Acid / Q000096:biosynthesis*; D007780:Lacticaseibacillus casei / Q000378:metabolism; D008297:Male; D009584:Nitrogen / Q000378:metabolism; D012756:Sheep; D013464:Sulfuric Acids
D003470:Culture Media; D007220:Industrial Waste; D013464:Sulfuric Acids; D019344:Lactic Acid; D009584:Nitrogen; C033158:sulfuric acid; D006851:Hydrochloric Acid