[Down regulatory effects of platelet factor four (PF4) on total adherence and respiratory burst of human neutrophil].
OBJECTIVE
The effect of platelet factor four (PF4) on human neutrophil function was studied.


METHODS
Crystal violet dye staining, immunofluorescence labeling, PKC kit assay, NBT and HVA fluoro-spectrophotometry were applied to study the effect of PF4 on total adherence, integrin CD11b level, PKC level and respiratory burst level of resting human neutrophils and FMLP/or PMA-stimulated human neutrophils.


RESULTS
It was found that PF4 slightly increased the total adherence of resting human neutrophils. There was no change on integrin CD11b level and respiratory burst level of resting human neutrophils after interaction with PF4. However, PF4 significantly down-regulated the total adherence, integrin CD11b level and respiratory burst level of human neutrophils stimulated by FMLP/or PMA. In addition, PF4 did not influence PKC level on resting and activated human neutrophil.


CONCLUSIONS
These results indicate that PF4 plays a down regulation of the function of human neutrophils. Differing from other members of classical CXC-chemokine family, the signaling of PF4 is not through PLC-PKC signal pathway.
Lu
S H
SH
National Laboratory of Experimental Hematology, Institute of Hematology, CAMS, PUMC, Tianjin 300020, China.
Liu
Y J
YJ
Ma
Y X
YX
Yang
L
L
Han
Z C
ZC
D039481:CD11b Antigen / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D015536:Down-Regulation; D006801:Humans; D016023:Integrins / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D010978:Platelet Factor 4 / Q000494:pharmacology*; D011493:Protein Kinase C / Q000378:metabolism; D016897:Respiratory Burst / Q000187:drug effects*
D039481:CD11b Antigen; D016023:Integrins; D010978:Platelet Factor 4; D011493:Protein Kinase C