Surface trapping and AFM detection of DNA topological intermediates generated from an oxidative chemical nuclease.
Direct observation of DNA topological intermediates generated from a 'chemical nuclease' treatment has been made by atomic force microscopy (AFM). The intermediates were trapped at the mica-water interface and imaging was carried out in the dynamic force mode. Complete conversion from supercoiled circular state to relaxed circular/linear state has been observed over a time scale of 8 min. Implication of such studies in complementing gel electrophoresis data has been predicted.
Mukhopadhyay
R
R
Department of Biological Sciences and Bioengineering, Indian Institute of Technology Kanpur, Kanpur, Uttar Pradesh 208016, India. rupam@iitk.ac.in
Srivatsan
S G
SG
Verma
S
S
D000538:Aluminum Silicates / Q000737:chemistry; D004247:DNA / Q000737:chemistry / Q000648:ultrastructure*; D018625:Microscopy, Atomic Force; D009690:Nucleic Acid Conformation; D010084:Oxidation-Reduction; D013499:Surface Properties; D014867:Water / Q000737:chemistry
D000538:Aluminum Silicates; D014867:Water; D004247:DNA; C011934:mica