title: [Gene therapy of rat prolactinomas mediated by adenoviral vectors with rat tyrosine hydroxylase gene].
abstract: OBJECTIVE
  To investigate the potential of gene therapy of rat prolactinomas mediated by adenoviral vectors with a gene encoding rat tyrosine hydroxylase.
  
  
  METHODS
  Recombinant replication-deficient adenovirus named Ad-GFP-TH with rat TH-cDNA and control adenovirus named Ad-GFP were constructed by homologous recombination in bacterial cells. The rat pituitary prolactinoma cell line MMQ are chosen as the target cells to study the effect of gene therapy on their growth and prolactin secretion mediated by Ad-GFP-TH.
  
  
  RESULTS
  Recombinant Ad-GFP-TH and Ad-GFP were successfully reconstructed. Transfection of MMQ cells with Ad-GFP-TH not only restrained their growth but also decreased their PRL secretion.
  
  
  CONCLUSIONS
  Gene therapy may serve for a potential treatment for prolactinomas, especially invasive prolactinomas.
authors.affiliation: Department of Neurosurgery, PUMC Hospital, CAMS, PUMC, Beijing 100730, China. xuzhq@csc.pumch.ac.cn
authors.forename: Zhi-qin
authors.initials: ZQ
authors.lastname: Xu
authors.forename: Chang-bao
authors.initials: CB
authors.lastname: Su
authors.forename: Song-sen
authors.initials: SS
authors.lastname: Chen
authors.forename: Zu-yuan
authors.initials: ZY
authors.lastname: Ren
authors.forename: Xu
authors.initials: X
authors.lastname: Di
authors.forename: Wen-bin
authors.initials: WB
authors.lastname: Ma
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D015316:Genetic Therapy; D005822:Genetic Vectors; D010911:Pituitary Neoplasms / Q000628:therapy*; D015175:Prolactinoma / Q000628:therapy*; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection; D014446:Tyrosine 3-Monooxygenase / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D011994:Recombinant Proteins; D014446:Tyrosine 3-Monooxygenase