title: UniFrag and GenomePrimer: selection of primers for genome-wide production of unique amplicons.
abstract: The complementary programs UniFrag and GenomePrimer were developed to provide a reliable high-throughput method to select the most unique regions within genomic DNA sequence(s) and design primers therein, involving minimal user intervention and maximum flexibility.
authors.affiliation: Molecular Genetics, University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute, PO Box 14, 9750 AA Haren, The Netherlands.
authors.forename: Sacha A F T
authors.initials: SA
authors.lastname: van Hijum
authors.forename: Anne
authors.initials: A
authors.lastname: de Jong
authors.forename: Girbe
authors.initials: G
authors.lastname: Buist
authors.forename: Jan
authors.initials: J
authors.lastname: Kok
authors.forename: Oscar P
authors.initials: OP
authors.lastname: Kuipers
mesh_terms: D000465:Algorithms; D017931:DNA Primers / Q000138:chemical synthesis / Q000737:chemistry* / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D016415:Sequence Alignment / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*; D012984:Software
chemical_list: D017931:DNA Primers