Epitope map for a growth hormone receptor agonist monoclonal antibody, MAb 263.
Monoclonal antibody (MAb) 263 is a widely used monoclonal antibody that recognizes the extracellular domain (ECD) of the GH receptor. It has been shown to act as a GH agonist both in vitro and in vivo, and we report here that it must be divalent to exert its effect on the full-length receptor. To understand the mechanism of its agonist action, we have determined the precise epitope for this antibody using a novel random PCR mutagenesis approach together with expression screening in yeast. A library of 5200 clones of rabbit GH receptor ECD mutants were screened both with MAb 263 and with an anticarboxy-tag antibody to verify complete ECD expression. Sequencing for clones that expressed complete ECD but were not MAb 263 positive identified 20 epitope residues distributed in a discontinuous manner throughout the ECD. The major part of the epitope, as revealed after mapping onto the crystal structure model of the ECD molecule, was located on the side and upper portion of domain 1, particularly within the D-E strand disulfide loop 79-96. Molecular dynamics docking of an antibody of the same isotype as MAb 263 was used to dock the bivalent antibody to the 1528-A2 epitope and to visualize the likely consequences of MAb binding. The minimized model enables the antibody to grasp two receptors in a pincer-like movement from opposite sides, facilitating alignment of the receptor dimerization domains in a manner similar to, but not identical with, GH.
Wan
Yu
Y
School of Biomedical Sciences and Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland, Australia.
Zheng
Yuan Zhi
YZ
Harris
Jonathan M
JM
Brown
Richard
R
Waters
Michael J
MJ
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology* / Q000494:pharmacology*; D000918:Antibody Specificity; D018360:Crystallography, X-Ray; D004220:Disulfides / Q000378:metabolism; D018604:Epitope Mapping; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology; D007074:Immunoglobulin G / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D011487:Protein Conformation; D011817:Rabbits; D011986:Receptors, Somatotropin / Q000819:agonists* / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D016415:Sequence Alignment; D014364:Tryptophan / Q000235:genetics
D000911:Antibodies, Monoclonal; D004220:Disulfides; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D011986:Receptors, Somatotropin; D014364:Tryptophan