title: Unexpected high testis-specific transcriptional activity of the cyclin T1 promoter in transgenic mice.
abstract: The ubiquitously expressed cyclin T1 gene encodes for a protein involved in human immunodeficiency virus type 1 (HIV-1) transcription activation. The goat gene was recently shown to share an expression pattern similar to that of its endogenous counterpart when incorporated into mice using a BAC insert. To assess if its promoter could target ubiquitous expression of the bovine Prnp in transgenic mice, two constructs carrying either 1 or 30 kb of cyclin T1 5'-flanking sequences were built and microinjected. Both constructs resulted in the unexpected high male germ cell-specific expression of the prion protein. These data re-question the suspected location of the cyclin T1 gene regulatory elements.
authors.affiliation: Laboratoire de Génétique Biochimique et de Cytogénétique, INRA, 78352 Cedex, Jouy-en-Josas, France. mata@jouy.inra.fr
authors.forename: X
authors.initials: X
authors.lastname: Mata
authors.forename: N
authors.initials: N
authors.lastname: Besnard
authors.forename: K
authors.initials: K
authors.lastname: Le Roux
authors.forename: G
authors.initials: G
authors.lastname: Tilly
authors.forename: O
authors.initials: O
authors.lastname: Andréoletti
authors.forename: M
authors.initials: M
authors.lastname: Hudrisier
authors.forename: J
authors.initials: J
authors.lastname: Costa Da Silva
authors.forename: H
authors.initials: H
authors.lastname: Laude
authors.forename: J L
authors.initials: JL
authors.lastname: Vilotte
mesh_terms: D024506:5' Flanking Region; D000818:Animals; D002417:Cattle; D056906:Cyclin T; D016213:Cyclins / Q000235:genetics* / Q000378:metabolism; D005822:Genetic Vectors; D005854:Germ Cells / Q000378:metabolism; D006041:Goats; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D008845:Microinjections; D011328:Prions / Q000096:biosynthesis / Q000235:genetics; D011401:Promoter Regions, Genetic; D013737:Testis / Q000166:cytology / Q000378:metabolism*; D014018:Tissue Distribution; D014158:Transcription, Genetic
chemical_list: C503684:CCNT1 protein, human; C503685:Ccnt1 protein, mouse; D056906:Cyclin T; D016213:Cyclins; D011328:Prions