Beta-amyloid peptides in cerebrospinal fluid of patients with Creutzfeldt-Jakob disease.
Decreased levels of beta-amyloid peptide 1-42 (Abeta1-42) in cerebrospinal fluid (CSF) are a characteristic feature of Alzheimer's disease (AD) but recently were also observed in Creutzfeldt-Jakob disease (CJD). We analyzed the CSF of patients with CJD, and AD and nondemented controls using a quantitative urea-based Abeta sodium dodecyl sulfate polyacrylamide gel electrophoresis immunoblot. Like in AD and nondemented controls, we found a highly conserved pattern of carboxyterminally truncated Abeta1-37/38/39 in addition to Abeta1-40/42 also in CJD patients. By the introduction of the ratio Abeta1-39 to Abeta1-42, CJD and AD can effectively be differentiated. We conclude that the immunoblot shows disease-specific CSF Abeta peptide patterns in CJD and AD and suppose that measurement of the Abeta peptide pattern seems to be a promising diagnostic tool in the differential diagnosis of dementias.
Wiltfang
Jens
J
Department of Psychiatry, University Erlangen-Nürnberg, Germany.
Esselmann
Hermann
H
Smirnov
Alexander
A
Bibl
Mirko
M
Cepek
Lukas
L
Steinacker
Petra
P
Mollenhauer
Brit
B
Buerger
Katharina
K
Hampel
Harald
H
Paul
Sabine
S
Neumann
Manuela
M
Maler
Manuel
M
Zerr
Inga
I
Kornhuber
Johannes
J
Kretzschmar
Hans A
HA
Poser
Sigrid
S
Otto
Markus
M
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000134:cerebrospinal fluid; D016229:Amyloid beta-Peptides / Q000134:cerebrospinal fluid*; D001057:Apolipoproteins E / Q000134:cerebrospinal fluid; D007562:Creutzfeldt-Jakob Syndrome / Q000134:cerebrospinal fluid*; D004591:Electrophoresis, Polyacrylamide Gel; D004660:Encephalitis / Q000134:cerebrospinal fluid; D005260:Female; D006801:Humans; D015151:Immunoblotting; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000134:cerebrospinal fluid; D016875:tau Proteins / Q000134:cerebrospinal fluid
D016229:Amyloid beta-Peptides; D001057:Apolipoproteins E; D010446:Peptide Fragments; D016875:tau Proteins