Mixed-element capture agents: a simple strategy for the construction of synthetic, high-affinity protein capture ligands.
Demonstration of a simple strategy to generate synthetic high-affinity protein capture agents of practical utility for protein-detecting microarrays. The model study highlights capture of the MBP-Mdm2 fusion protein on a solid support by a linear sequence of peptides that bind to the two individual polypeptide chains.
Bachhawat-Sikder
Kiran
K
Center for Biomedical Inventions and the Department of Internal Medicine, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390-8573, USA.
Kodadek
Thomas
T
D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D008024:Ligands; D058619:Maltose-Binding Proteins; D009687:Nuclear Proteins; D011485:Protein Binding; D040901:Proteomics / Q000379:methods*; D011518:Proto-Oncogene Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D051736:Proto-Oncogene Proteins c-mdm2; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D002352:Carrier Proteins; D008024:Ligands; D058619:Maltose-Binding Proteins; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; D011993:Recombinant Fusion Proteins; D051736:Proto-Oncogene Proteins c-mdm2