title: The factor C3 conversion in human complement by smooth Shigella flexneri lipopolysaccharides.
abstract: Shigella flexneri rods play an important role in human intestinal infections. In the presented studies we have shown that O-acetyl and glucose residues, substituted in main GalNAc-Rha chains of lipopolysaccharide (LPS) are important for the bactericidal effect of human serum. By dot-blot, immunoblotting and ELISA with immobilized LPS we have shown correlation of C3 fragments deposition and serum resistance. LPSs isolated from a serum-sensitive strain deposited more C3 fragments than LPSs from serum-resistant Shigella flexneri strains.
authors.affiliation: Institute of Microbiology and Immunology, University of Lódź, Poland.
authors.forename: Rafał
authors.initials: R
authors.lastname: Fudała
authors.forename: Włodzimierz
authors.initials: W
authors.lastname: Doroszkiewicz
authors.forename: Joanna
authors.initials: J
authors.lastname: Niedbach
authors.forename: Andrzej
authors.initials: A
authors.lastname: Gamian
authors.forename: Andrej
authors.initials: A
authors.lastname: Weintraub
authors.forename: Wiesław
authors.initials: W
authors.lastname: Kaca
mesh_terms: D001770:Blood Bactericidal Activity / Q000276:immunology; D002240:Carbohydrate Sequence; D003167:Complement Activation / Q000276:immunology*; D003176:Complement C3 / Q000276:immunology* / Q000378:metabolism; D015941:Complement Hemolytic Activity Assay; D004405:Dysentery, Bacillary / Q000097:blood / Q000276:immunology* / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting; D008070:Lipopolysaccharides / Q000276:immunology*; D008969:Molecular Sequence Data; D019081:O Antigens / Q000276:immunology; D012763:Shigella flexneri / Q000276:immunology*
chemical_list: D003176:Complement C3; D008070:Lipopolysaccharides; D019081:O Antigens