Effect of secondary structure of short double-stranded RNA on RNAi efficiency.
The dangling nucleotides are very important for the RNA interference (RNAi). We investigate the effect of the dangling end on both the thermodynamic property of the RNA helix and efficiency of gene regulation in RNAi pathway. The result indicates that the four dangling adenines, which are more stable than two or three dangling residues, is not suitable for the RNAi efficiency.
Ohmichi
Tatsuo
T
High Technology Research Center, Department of Chemistry, Faculty of Science and Engineering, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan.
Karimata
Hisae
H
Sugimoto
Naoki
N
D005786:Gene Expression Regulation; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry*; D013816:Thermodynamics
D012313:RNA