Expression of immunogenic S1 glycoprotein of infectious bronchitis virus in transgenic potatoes.
The expression of infectious bronchitis virus (IBV) S1 glycoprotein in potatoes and its immunogenicity in mice and chickens were investigated. Potato plants were genetically transformed with a cDNA construct encoding the IBV S1 glycoprotein with the Agrobacterium system. Genomic DNA and mRNA analyses of the transformed plantlets confirmed the integration of the foreign cDNA into the potato genome, as well as its transcription. Mice and chickens vaccinated with the expressed IBV S1 glycoprotein produced antibodies that neutralized IBV infectivity. After three immunizations, vaccinated chickens were completely protected from virulent IBV infection. These results demonstrate that transgenic potatoes expressing IBV S1 glycoprotein can be used as a source of recombinant antigen for vaccine production.
Zhou
Ji-Yong
JY
Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310029, China.
Wu
Jian-Xiang
JX
Cheng
Li-Qin
LQ
Zheng
Xiao-Juan
XJ
Gong
Hui
H
Shang
Shao-Bin
SB
Zhou
En-Min
EM
D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary; D006023:Glycoproteins / Q000235:genetics*; D001351:Infectious bronchitis virus / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000821:virology*; D011198:Solanum tuberosum / Q000821:virology*
D017931:DNA Primers; D018076:DNA, Complementary; D006023:Glycoproteins