Calcium alginate gel as encapsulation matrix for coimmobilized enzyme systems.
Encapsulation within calcium alginate gel capsules was used to produce a coimmobilized enzyme system. Glucose oxidase (GOD) and catalase (CAT) were chosen as model enzymes. The same values of Vmax and Km app for the GOD encapsulated system and for the GOD-CAT coencapsulated system were calculated. When gel beads and capsules were compared, the same catalyst deactivation sequence for the two enzymes was observed. However, when capsules were employed as immobilization support, GOD efficiencies were higher than for the gel beads. These results were explained in terms of the structure of the capsules.
Blandino
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Biological and Enzymatic Reactors Research Group, Department of Chemical Engineering, Food Technology and Environmental Technologies, Faculty of Sciences, University of Cádiz, 11510 Puerto Real (Cádiz), Spain. ana.blandino@uca.es
Macías
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Cantero
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D000464:Alginates / Q000737:chemistry*; D002118:Calcium / Q000737:chemistry*; D002374:Catalase / Q000737:chemistry; D004798:Enzymes / Q000737:chemistry*; D004800:Enzymes, Immobilized; D005782:Gels / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D007700:Kinetics; D008956:Models, Chemical; D013997:Time Factors
D000464:Alginates; D004798:Enzymes; D004800:Enzymes, Immobilized; D005782:Gels; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D002374:Catalase; D005947:Glucose; D002118:Calcium