[Neuroprotective effects of Tripterygium Wilforddi Hook F monomer T10 on glutamate induced PC12 cell line damage and its mechanism].
OBJECTIVE
To study the neuroprotective effect and related mechanisms of triptolide.


METHODS
After being treated with 10(-10), 10(-9) mol.L-1 triptolide and/or 10, 50 mmol.L-1 glutamate for 24 hours, and the viability of PC12 was detected by MTT conversion, and the apoptosis rate was detected by AnnexinV-FITC and PI staining. In order to understand the underlying mechanism of the neuroprotective effect of triptolide, H2DCFDA and JC1 were used to detect the reactive oxygen species (ROS) and mitochondrial membrane potential.


RESULTS
Glutamate could induce PC12 necrosis and apoptosis. T10 at 10(-10) or 10(-9) mol.L-1 inhibited glutamate-induced cell death, ROS formation and decrease of mitochondrial membrane potential.


CONCLUSIONS
T10 at 10(-10) or 10(-9) mol.L-1 can protect PC12 from the death induced by glutamate; and the underlying mechanisms may be involved in the inhibition of ROS formation and decrease of mitochondrial membrane potential.
He
Qihua
Q
Neuroscience Research Institute, Peking University, Beijing 100083, China.
Zhou
Huifang
H
Xue
Bing
B
Niu
Dongbin
D
Wang
Xiaomin
X
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D004224:Diterpenes / Q000494:pharmacology*; D004852:Epoxy Compounds; D018698:Glutamic Acid / Q000633:toxicity*; D008564:Membrane Potentials / Q000187:drug effects; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016716:PC12 Cells; D010616:Phenanthrenes; D051381:Rats; D017382:Reactive Oxygen Species
D004224:Diterpenes; D004852:Epoxy Compounds; D018696:Neuroprotective Agents; D010616:Phenanthrenes; D017382:Reactive Oxygen Species; C001899:triptolide; D018698:Glutamic Acid