[Degradation of streptokinase and the catalytic properties of the plasmin-streptokinase complex].
Streptokinase (SK) interacts with human plasminogen (Pg) or plasmin (Pm) with formation of Pg-SK or Pm-SK complex. Pm-SK complex manifests a fibrinolytic, amidolytic and Pg activator activity. SK in complex with Pm isn't stable and so capable to be hydrolysed rapidly. We investigated a correlation between molecular form of SK and catalytic properties of equimolar Pm-SK complex during preincubation at 20 degrees C. It was found out that amidolytic activity of Pm-SK complex was not changing for 5 hours and decreased to the initial Pm value after 24 hours. During this time alpha 2-antiplasmin (alpha 2-AP) has any effect on amidolytic activity of the complex. Fibrinolytic activity of Pm-SK complex makes up 20% of the initial Pm value and wasn't changing within the investigated period. Pg activator activity was decreasing rapidly to 30-40% of the initial one within few minutes from the moment of Pm-SK complex formation. It was 10-20% of that initial after 24 hours. The decrease in Pg activator activity of Pm-SK complex correlated with the initial very rapid conversion of 47 kDa SK to 36 kDa SK within few minutes and following more slow conversion of SK in 31, 25 and 15 kDa fragments after 5 hours. alpha 2-AP didn't influence on the Pg activator activity of Pm-SK complex but eliminated its fibrinolytic activity completely. It was supposed that alpha 2-AP inhibited fibrinolytic activity of Pm-SK complex similarly to 6-aminohexanoic acid by preventing Pm-SK complex binding to fibrin polymer.
Hrynenko
T V
TV
Palladin Institute of Biochemistry, NAS of Ukraine, Kyiv. grinenko@biochem.kiev.ua
Makohonenko
Ie M
IeM
Iusova
O I
OI
Cederholm-Williams
S A
SA
D002384:Catalysis; D004795:Enzyme Stability; D005341:Fibrinolysin / Q000737:chemistry*; D005342:Fibrinolysis / Q000187:drug effects; D006801:Humans; D007700:Kinetics; D008970:Molecular Weight; D010958:Plasminogen / Q000737:chemistry; D011485:Protein Binding; D013300:Streptokinase / Q000737:chemistry / Q000378:metabolism*; D013329:Structure-Activity Relationship; D010959:Tissue Plasminogen Activator / Q000737:chemistry; D000979:alpha-2-Antiplasmin / Q000737:chemistry
D000979:alpha-2-Antiplasmin; D010958:Plasminogen; D013300:Streptokinase; D010959:Tissue Plasminogen Activator; D005341:Fibrinolysin