Study on the (14)C-glucose metabolism by Clonorchis sinensis: Paper Chromatographic Analyses in Combination with Autoradiography.
Radioactive (14)C-glucose(U) was given to Clonorchis sinensis in Tris buffer medium, in corder to trace the metabolic fate of the labelled carbon. The labelled carbon from glucose enters into intermediary metabolites and end products of anaeroblic glycolysis, Embden-Meyerhof pathway, and of aerobic Krebs cycle. These product were identified by one or two-dimensional paper chromatography in combination with autoradoigraphy. 1)The labelled metabolites detected in this experiment corresponded to pyruvic acid, latic acid, malic acid, succinic acid and fumaric acid. 2)Amino acids, such as alanine, aspartic acid, glutamic acid, valine, theronine, and serine, derived by the degradation of (14)C- glycose were also found. 3)Labelled compounds behaving like alanine, aspartic acid and glutamic acid were observed in the chroma to gram of incubation medium. 4)The preciptation which suggests a positive reaction for protein occured when absolute ethanol was added to the incubation medium.
Kang
Il Kwon
IK
Department of Parasitology and Institute of Endemic Diseases, College of Medicine, Seoul National University, Korea.
Lee
Soon Hyung
SH
Seo
Byong Seol
BS