title: Synthesis of 2-5As possessing base-modified adenosines and their activities to human recombinant RNase L.
abstract: The unique 2',5'-oligoadenylate (2-5A) acts as a potent inhibitor of translation in vertebrate cells through the activation of a constituent latent 2-5A-dependent endoribonuclease (RNase L). This 2-5A system plays a major role in the interferon natural defense mechanism against viral infection. We report the syntheses of base-modified adenosine-substituted 2-5A derivatives, their interaction with recombinant human RNase L and their biological stability.
authors.affiliation: Laboratory of Molecular Biochemistry, Department of Biomolecular Science, Faculty of Engineering, Gifu University, Yanagido 1-1, Gifu 501-1193, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kitade
authors.forename: Y
authors.initials: Y
authors.lastname: Tanida
authors.forename: T
authors.initials: T
authors.lastname: Tuboi
authors.forename: A
authors.initials: A
authors.lastname: Yoshimura
authors.forename: M
authors.initials: M
authors.lastname: Nakanishi
authors.forename: C
authors.initials: C
authors.lastname: Yatome
mesh_terms: D000227:Adenine Nucleotides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D000241:Adenosine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D004722:Endoribonucleases / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D015394:Molecular Structure; D009843:Oligoribonucleotides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D000227:Adenine Nucleotides; D009843:Oligoribonucleotides; D011994:Recombinant Proteins; C023505:2',5'-oligoadenylate; D004722:Endoribonucleases; C031956:2-5A-dependent ribonuclease; D000241:Adenosine