A peptide antibody for rapid screening of Streptomyces species producing phospholipase D.
By examining the conserved regions in the protein sequences of eight different Streptomyces phospholipase Ds (PLD) reported so far and the X-ray crystallographic structure of a Streptomyces PLD, we designed a peptide sequence, DPANRGAVGSGGYSQIKSL, for the screening of microorganisms producing PLD. In the enzyme-linked immunosorbent assay using a mouse antibody raised against the designed peptide, we recovered seven producing strains out of 128 soil isolates.
Uhm
Tai-Boong
TB
Faculty of Biological Sciences and Institute for Molecular Biology and Genetics, Chonbuk National University, Jeonju 561-756, Republic of Korea. tbuhm@moak.chonbuk.ac.kr
Lee
Sun Hee
SH
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents; D017124:Conserved Sequence; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004789:Enzyme Activation; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D008969:Molecular Sequence Data; D010455:Peptides; D010739:Phospholipase D / Q000032:analysis* / Q000096:biosynthesis*; D015202:Protein Engineering / Q000379:methods; D020539:Sequence Analysis, Protein / Q000379:methods; D013045:Species Specificity; D013302:Streptomyces / Q000145:classification / Q000201:enzymology* / Q000302:isolation & purification*
D000900:Anti-Bacterial Agents; D010455:Peptides; D010739:Phospholipase D