[Cisplatin-induced apoptotic cell death in spiral ganglion and organ of Corti of mongolian gerbil cochlear].
OBJECTIVE
To investigate whether apoptosis is one of the mechanism for cell death in spiral ganglion(SG) and organ of Corti of Mongolian gerbils cochlea induced by cisplatin.


METHODS
Mongolian gerbils were administered 4 mg.kg-1.d-1 cisplatin consecutively for 4 to 7 days. The apoptotic cell death in spiral ganglion and organ of Corti of cochlea basal turn was detected via transmission electron microscopy and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) method.


RESULTS
The observation of electron microscopy shown special morphological change consistent with the feature of cell apoptosis in some spiral ganglion cells and outer hair cells after 5-7 days' cisplatin treatment. The apoptotic labeling by TUNEL in spiral ganglion and organ of Corti was detected after 5 days' treatment and increased after 6 and 7 days' treatment whereas almost negative result were obtained in the normal control animals.


CONCLUSIONS
Apoptosis is one of the important mechanisms for cell injury of spiral ganglion and organ of Corti induced by cisplatin.
Xu
Hui-jie
HJ
Department of Otorhinolaryngology, Beijing Hospital, Beijing 100730, China. xuhuijie@yahoo.com
Huang
Wei-ning
WN
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002945:Cisplatin / Q000633:toxicity*; D003051:Cochlea / Q000166:cytology / Q000187:drug effects*; D005260:Female; D005849:Gerbillinae; D020287:In Situ Nick-End Labeling; D009474:Neurons / Q000187:drug effects; D009925:Organ of Corti / Q000166:cytology* / Q000187:drug effects; D013136:Spiral Ganglion / Q000166:cytology* / Q000187:drug effects
D002945:Cisplatin