[Influence of adjustment of balance of Th1/Th2 type cytokines on proliferation of glioma cells].
OBJECTIVE
To study the influence of adjustment of balance of Th1/Th2 by external cytokines on proliferation of glioma cells.


METHODS
The gene expressions of Th1/Th2 type cytokines in C6, 9L, U251 and SHG44 glioma cells were detected by semiquantitative reverse transcription polymerase chain reaction (RT-PCR). After the cells were induced with IFN-gamma + IL-4 McAb and IL-4 + IFN-gamma McAb respectively, we isolated the total RNA to proceed RT-PCR again. The evaluation of cell proliferation was proceeded by MTT assay method.


RESULTS
There was obviously predominant expression of Th2 type cytokines in glioma cell lines (P < 0.01). The expression intensity of IFN-gamma was improved in IFN-gamma + IL-4 McAb groups and Th2 type cytokines were enhanced in IL-4 + IFN-gamma McAb groups. IFN-gamma and IL-4 McAb could cause the switch from Th2 to Th1, and could remarkably inhibit the proliferation of glioma cells in a dose-dependent way (P < 0.01). On the other hand, IL-4 and IFN-gamma McAb could strengthen the switch of Th2, and might stimulate the glioma cell growth, also in a dose-dependent way (P < 0.01).


CONCLUSIONS
There is a Th2 preponderance in glioma cells. IFN-gamma and IL-4 McAb could regulate the switch from Th2 to Th0 or Th1, and inhibit the proliferation of glioma cells.
Hu
Y S
YS
Department of Neurosurgery, Second Hospital of Shandong University, Jinan 250033, China. hysh69@hotmail.com
Zhang
Q L
QL
Tian
Z G
ZG
Wei
H M
HM
Zhang
J H
JH
Li
G
G
Pang
Q
Q
Wang
C W
CW
Jin
P
P
Sun
R
R
D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D001932:Brain Neoplasms / Q000276:immunology* / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D016207:Cytokines / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects; D005910:Glioma / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology; D015847:Interleukin-4 / Q000235:genetics / Q000276:immunology; D007378:Interleukins / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000911:Antibodies, Monoclonal; D016207:Cytokines; D007378:Interleukins; D015847:Interleukin-4; D007371:Interferon-gamma