title: Antiproliferative effect of chlorophyllin derived from a traditional Chinese medicine Bombyx mori excreta on human breast cancer MCF-7 cells.
abstract: Chlorophyllin (CHL) is the sodium-copper salts of chlorophyll derivatives. In this study, the CHL was derived from a traditional Chinese medicine, the Bombyx mori excreta. The CHL, at concentrations 25-400 microg/ml, reduced the proliferation of HL-60, K-562, S-180, and MCF-7 cells by 8.2-95.7% after 72 h of incubation. The CHL also accumulated G2/M cells and induced apoptosis in the MCF-7 cells. Furthermore, the breast carcinoma cells exhibited lower cyclin D1 and cyclin E levels but higher cyclin B1 level after incubation with the CHL showing that these cyclin changes may be important for the antiproliferative effect of the CHL.
authors.affiliation: Department of Biology, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR, P.R. China. chimingchiu@cuhk.edu.hk
authors.forename: Lawrence Chi-Ming
authors.initials: LC
authors.lastname: Chiu
authors.forename: Carrie K-L
authors.initials: CK
authors.lastname: Kong
authors.forename: Vincent E C
authors.initials: VE
authors.lastname: Ooi
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology; D015153:Blotting, Western; D012831:Bombyx / Q000378:metabolism*; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002448:Cell Adhesion; D002453:Cell Cycle; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D045744:Cell Line, Tumor; D002735:Chlorophyllides / Q000494:pharmacology*; D004396:Coloring Agents / Q000494:pharmacology; D019926:Cyclin B / Q000096:biosynthesis; D056744:Cyclin B1; D019938:Cyclin D1 / Q000096:biosynthesis; D019927:Cyclin E / Q000096:biosynthesis; D004247:DNA / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D005243:Feces; D005434:Flow Cytometry; D016195:G2 Phase; D049452:Green Fluorescent Proteins; D018922:HL-60 Cells; D006801:Humans; D020014:K562 Cells; D008164:Luminescent Proteins / Q000378:metabolism; D008516:Medicine, Chinese Traditional; D008938:Mitosis; D008956:Models, Chemical; D013997:Time Factors
chemical_list: D016587:Antimutagenic Agents; D000970:Antineoplastic Agents; C534699:CCNB1 protein, human; D002735:Chlorophyllides; D004396:Coloring Agents; D019926:Cyclin B; D056744:Cyclin B1; D019927:Cyclin E; D008164:Luminescent Proteins; D019938:Cyclin D1; D049452:Green Fluorescent Proteins; D004247:DNA; C007020:chlorophyllin; D001973:Bromodeoxyuridine