title: Bioanalytical considerations for compounds containing free sulfhydryl groups.
abstract: Thiol-containing compounds pose bioanalytical challenge in several dimensions due to extreme reactivity of the sulfhydryl group. The development of robust bioanalytical methodology for thiol groups should address the aspects of adequate stabilization in the biological matrix and selectivity considerations. In this context, availability of plethora of thiol reagents provides ample opportunity to achieve the above goals. However, several considerations need to be factored into the decision-making of a suitable scheme for the thiol drugs under investigation. This review provides some critical insights to many such considerations that may be vital for development and validation of methods for thiol-containing drugs.
authors.affiliation: Department of Bioanalysis, Drug Metabolism and Pharmacokinetics, Discovery Research, Dr Reddy's Research Laboratories, Bollaram Road, Miyapur, Hyderabad-500 050, India. nrsrinivas@drreddys.com
authors.forename: Nuggehally R
authors.initials: NR
authors.lastname: Srinivas
authors.forename: Rao N V S
authors.initials: RN
authors.lastname: Mamidi
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004355:Drug Stability; D006801:Humans; D015394:Molecular Structure; D004364:Pharmaceutical Preparations / Q000737:chemistry / Q000378:metabolism; D013379:Substrate Specificity; D013438:Sulfhydryl Compounds / Q000032:analysis / Q000737:chemistry*; D013439:Sulfhydryl Reagents / Q000737:chemistry
chemical_list: D004364:Pharmaceutical Preparations; D013438:Sulfhydryl Compounds; D013439:Sulfhydryl Reagents