[Stimulatory effect of chemokine-like factor 1 (CKLF1) on the growth of bone marrow cells].
OBJECTIVE
To investigate the activity of CKLF1 on the proliferation and differentiation of bone marrow cells.


METHODS
Human low density bone marrow cells and mouse bone marrow cells were plated in 96-well microplate and supernatants from transfected COS-7 cell culture were added. The cell proliferation was assayed by MTT method after 5 days incubation. The enhancing effect of CKLF1 on the colony formation of human hematopoietic progenitor cells was identified in semi-solid culture.


RESULTS
CKLF1 has obvious enhancing effect on both human and mouse bone marrow cells, it can stimulate the colony formation of human hematopoietic stem cells and has synergistic action with GM-CSF.


CONCLUSIONS
CKLF1 can promote the proliferation and differentiation of bone marrow cells.
Han
W L
WL
Department of Immunology, Basic Medical School, Peking University, Beijing 100083, China.
Rui
M
M
Zhang
Y M
YM
Chen
Y Y
YY
Zhong
Y C
YC
Di
C H
CH
Song
Q S
QS
Ma
D L
DL
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D018925:Chemokines / Q000494:pharmacology*; D003114:Colony-Forming Units Assay; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D062792:MARVEL Domain-Containing Proteins; D051379:Mice; D008807:Mice, Inbred BALB C
C432676:CKLF protein, human; C000593975:CKLF1 protein, mouse; D018925:Chemokines; D062792:MARVEL Domain-Containing Proteins