[Apoptosis of chondrocytes in cartilage].
OBJECTIVE
To observe apoptosis of chondrocytes in cartilage and arthritis.


METHODS
Apoptosis was detected by light and electron microscope and DNA electrophoresis, while [Ca2+]i was detected by confocal laser scanning microscope.


RESULTS
Cartilage cells from osteoarthritis (OA) and rheumatoid arthritis (RA) could be seen to undergo normal differentiation after cultured for 3 days under light or electron microscope, while some chondrocytes directly isolated without culturing could give early changes of apoptosis, and normal chondrocytes in vitro after treatment with IL-1, TNF-alpha also underwent apoptosis. IL-1, TNF-alpha could induce increase of [Ca2+]i in chondrocytes.


CONCLUSIONS
Our data showed that apoptosis might present in the normal cartilage as well as in OA and RA cells. IL-1, TNF-alpha could induce apoptosis of chondrocytes and [Ca2+]i increase during apoptosis.
Chen
Y
Y
Department of Cell Biology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China.
Wang
A
A
Zhou
J
J
Qiu
G
G
Zhang
J
J
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D001168:Arthritis / Q000473:pathology*; D002118:Calcium / Q000378:metabolism; D002358:Cartilage, Articular / Q000166:cytology*; D002417:Cattle; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology*; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D010281:Parathyroid Hormone / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D007375:Interleukin-1; D010281:Parathyroid Hormone; D014409:Tumor Necrosis Factor-alpha; D002118:Calcium