Analysis of single nucleotide polymorphisms in uridine/cytidine kinase gene encoding metabolic enzyme of 3'-ethynylcytidine.
We investigated single nucleotide polymorphisms (SNPs) in uck2 gene encoding metabolic enzyme of 3'-ethynylcytidine (ECyd) which were associated with drug response of ECyd, and the newly synthesized antitumor ribonucleoside analog. We analized that on exon-intron junction and exon region to affect the qualitative alteration of gene product directly in ECyd sensitive and resistant human cancer cell lines. As the results, cSNP and sSNP were detected in exon 4. In the promoter region, 3 SNPs were detected. Our data seem to be able to give an important knowledge, when ECyd is applied clinically.
Hasegawa
Takako
T
Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama 700-8530, Japan.
Futagami
Michiko
M
Kim
Hey-Sook
HS
Matsuda
Akira
A
Wataya
Yusuke
Y
D003562:Cytidine / Q000031:analogs & derivatives* / Q000378:metabolism*; D005091:Exons; D006801:Humans; D007438:Introns; D020641:Polymorphism, Single Nucleotide; D014407:Tumor Cells, Cultured; D014541:Uridine Kinase / Q000235:genetics*
C103084:1-(3-C-ethynylribopentofuranosyl)cytosine; D003562:Cytidine; D014541:Uridine Kinase