Anti-thrombotic activity of PDR, a newly synthesized L-Arg derivative, on three thrombosis models in rats.
The possibility of a newly synthesized L-arginine derivative, polyaspartoyl-L-arginine (PDR), as a novel anti-thrombotic agent and its mode of action were investigated. The anti-platelet effects of PDR in rats ex vivo, anti-thrombotic effects in three thrombosis models in rats and its effect on some autacoids (nitric oxide [NO], thromboxane [TXA2] and prostacyclin [PGI2]) were studied. PDR (i.g.) significantly inhibited ADP-, collagen- or thrombin-induced rat platelet aggregation. In arteriovenous shunt model and ferric chloride-induced arterial thrombosis model in rats, PDR (i.g.) significantly reduced the thrombus weight. In electrical stimulation-induced arterial thrombosis in rats, PDR (i.v.) dose-dependently prolonged the thrombus occlusion time (OT). PDR increased the concentration of NO in plasma. In contrast with aspirin (ASA), PDR did not influence on the TXA2 and PGI2 levels in plasma. In conclusion, PDR is provided with significant inhibitory effect on platelet aggregation and prevention effect on platelet related thrombosis, which is probably attributed to its inhibition on platelet function by L-arginine-NO pathway. The results demonstrate that PDR is a novel, oral and venous effective platelet aggregation inhibitor and has a possibility used as an anti-thrombotic agent.
Tang
Zhiyu
Z
School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100083, PR China.
Wang
Yinye
Y
Xiao
Yanliu
Y
Zhao
Ming
M
Peng
Shiqi
S
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives*; D001166:Arteriovenous Shunt, Surgical; D012898:Autacoids / Q000378:metabolism; D002712:Chlorides; D003094:Collagen / Q000494:pharmacology; D004558:Electric Stimulation; D005290:Ferric Compounds; D005343:Fibrinolytic Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D008297:Male; D010455:Peptides / Q000138:chemical synthesis / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000138:chemical synthesis / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013917:Thrombin / Q000494:pharmacology; D013927:Thrombosis / Q000139:chemically induced / Q000209:etiology / Q000503:physiopathology*
D012898:Autacoids; D002712:Chlorides; D005290:Ferric Compounds; D005343:Fibrinolytic Agents; D010455:Peptides; D010975:Platelet Aggregation Inhibitors; C483710:polyaspartoyl-L-arginine; D000244:Adenosine Diphosphate; D003094:Collagen; D001120:Arginine; D013917:Thrombin; C024555:ferric chloride