title: Improved molecular detection of dengue virus serotype 1 variants.
abstract: The dengue virus molecular typing method described by Lanciotti and coworkers (R. S. Lanciotti, C. H. Calisher, D. J. Gubler, G. J. Chang, and A. Vance-Vorndam, J. Clin. Microbiol. 30:545-551, 1992) is used worldwide for diagnosis and surveillance. However, it failed to detect DENV-1 variants in Cambodia due to a point mutation. We describe an improvement of the method that allows the detection of additional DENV-1 strains, including potential variants.
authors.affiliation: Institut Pasteur du Cambodge. National Dengue Control Programme, Ministry of Health. World Health Organization, Phnom Penh, Cambodia. jmreynes@pasteur-kh.org
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Reynes
authors.forename: Sivuth
authors.initials: S
authors.lastname: Ong
authors.forename: Channa
authors.initials: C
authors.lastname: Mey
authors.forename: Chantha
authors.initials: C
authors.lastname: Ngan
authors.forename: Stefan
authors.initials: S
authors.lastname: Hoyer
authors.forename: Amadou A
authors.initials: AA
authors.lastname: Sall
mesh_terms: D001483:Base Sequence; D003715:Dengue / Q000175:diagnosis / Q000517:prevention & control; D003716:Dengue Virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D005784:Gene Amplification; D005814:Genes, Viral; D005843:Geography; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction