Apolipoprotein(a) and cortical cerebral infarction.
OBJECTIVE
The present study aimed to elucidating the correlation of apolipoprotein(a) [apo(a)] with cerebral infarction at the levels of molecule and protein.


METHODS
The serum Lp(a) level, by means of ELISA, the polymorphism of apo(a) protein, by SDS-PAGE/Western blotting combined with silver staining assays, and the sequence polymorphisms in 5' control region, the first exon and intron of apo(a) gene, by PCR-SSCP/AFLP assays, were detected in 85 healthy controls and 12 cases of cortical cerebral infarction.


RESULTS
The serum Lp(a) level was markedly higher in the patients (152.59+/-3.41 mg/L) than that in the controls (56.21+/-3.67 mg/L) (t=4, 15, P<0. 001), even between the subjects with the same apo(a) phenotype. The frequency of low molecular weight phenotype was significantly higher in the patients than that in the controls (0.5238 vs. 0.2941). There were 2 sites of sequence variance in the 5' control region of apo(a) gene in our studied population, which were of significant difference between patients and controls, and were related to the variation of serum Lp(a) level.


CONCLUSIONS
Our study found that the low molecular weight phenotype of apo(a) was closely associated with cerebral infarction, suggested that the variation of serum Lp(a) level be determined by not only the size of apo(a) gene but also its sequence, which indicated that both the size and sequence of apo(a) are associated with the susceptibility to cerebral infarction.
Yingdong
Z
Z
Department of Neurology, Nanjing Brain Hospital, Nanjing Medical University, Nanjing 210029.
Xiuling
L
L
D000368:Aged; D001054:Apolipoproteins A / Q000097:blood* / Q000235:genetics; D002544:Cerebral Infarction / Q000097:blood* / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D017270:Lipoprotein(a) / Q000097:blood; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational
D001054:Apolipoproteins A; D017270:Lipoprotein(a)