title: Identification of Prevotella in pedal osteomyelitis of a diabetic patient.
abstract: Osteomyelitis in a diabetic patient with a nonhealing foot ulcer, multiple medical conditions, and recurrent hospitalization for antibiotic therapy was found to be associated with gram-negative bacteria Prevotella melanginoganica and Prevotella melaninoganica hemagglutinating variant. Those organisms were identified due to the morphologically distinct features in electron microscopy and sequencing of the genes after Polymerase chain reaction amplification from the pathological material. The bacteria invaded the bone and resided in osteocyte, osteoblast, and endothelial cells. The bacteria are usually associated with periodontal plaques, causing inflammation and destruction of gingival tissue and resorption of the alveolar bone. This is the first ultrastructural and molecular study of a diabetic bone lesion with anaerobic bacterial infection.
authors.affiliation: Department of Pathology, SUNY Downstate Medical Center, Brooklyn, New York 11222, USA.
authors.forename: Barbara J
authors.initials: BJ
authors.lastname: Dominiak
authors.forename: William
authors.initials: W
authors.lastname: Oxberry
authors.forename: Patrick C
authors.initials: PC
authors.lastname: Chen
mesh_terms: D016866:Bacteroidaceae Infections / Q000382:microbiology* / Q000473:pathology; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000032:analysis; D003922:Diabetes Mellitus, Type 1 / Q000150:complications / Q000473:pathology; D017719:Diabetic Foot / Q000382:microbiology* / Q000473:pathology; D005260:Female; D006801:Humans; D008854:Microscopy, Electron; D008875:Middle Aged; D008969:Molecular Sequence Data; D010019:Osteomyelitis / Q000382:microbiology* / Q000473:pathology; D016133:Polymerase Chain Reaction; D001443:Prevotella melaninogenica / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial