The effect of nasal polyp epithelial cells on eosinophil activation.
OBJECTIVE
Eosinophil infiltration into an inflammatory site is a characteristic histological finding in patients with chronic rhinosinusitis and nasal polyps. Most of the eosinophils in chronic rhinosinusitis are activated in the nasal cavity, but the exact activation mechanism of eosinophils is unknown. The study was designed to investigate the effect of human nasal epithelial cells on the activation of eosinophils.


METHODS
Peripheral blood eosinophils were isolated from healthy volunteers and incubated in human nasal polyp epithelial cell conditioned media (HPECM). Superoxide production and eosinophil-derived neurotoxin were measured to determine eosinophils activation. HPECMs were assayed by ELISAs for interleukin-8 (IL-8), granulocyte-macrophage colony stimulating factor (GM-CSF), eotaxin, and regulated on activation normal T expressed and secreted (RANTES). To identify the chemical mediators involved in the activation of eosinophils.


RESULTS
HPECM (n = 7) contained 31.48 ng/mL interleukin-8, 533.43 pg/mL GM-CSF, 5.90 pg/mL eotaxin, and 11.06 pg/mL RANTES. Eosinophils were activated by HPECM and inhibited only by anti-GM-CSF antibody, not by the other chemical mediators.


CONCLUSIONS
The results suggest that eosinophils in nasal secretions are activated by GM-CSF, which is produced by nasal epithelial cells.
Shin
Seung-Heon
SH
Department of Otolarynology, School of Medicine, Catholic University of Daegu, South Korea. hsseung@cataegu.ac.kr
Lee
Sang-Heon
SH
Jeong
Hyo-Soon
HS
Kita
Hirohito
H
D002478:Cells, Cultured; D054413:Chemokine CCL11; D018946:Chemokine CCL5 / Q000378:metabolism; D019742:Chemokines, CC / Q000378:metabolism; D017077:Culture Media, Conditioned; D047208:Eosinophil-Derived Neurotoxin; D004804:Eosinophils / Q000378:metabolism / Q000502:physiology*; D004847:Epithelial Cells / Q000378:metabolism / Q000502:physiology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D009298:Nasal Polyps / Q000503:physiopathology*; D012260:Ribonucleases / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
C515456:CCL11 protein, human; D054413:Chemokine CCL11; D018946:Chemokine CCL5; D019742:Chemokines, CC; D017077:Culture Media, Conditioned; D016209:Interleukin-8; D013481:Superoxides; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D047208:Eosinophil-Derived Neurotoxin; D012260:Ribonucleases