Determination of L-hyoscyamine in atropine and D-hyoscyamine in L-hyoscyamine by chiral capillary electrophoresis as an alternative to polarimetry.
A method for the separation of atropine enantiomers, D- and L-hyoscyamine by capillary electrophoresis (CE) has been developed and validated. The advantages of the CE method compared with polarimetry include smaller amounts of analytes and a lower limit of detection of the unwanted enantiomer. Moreover, the present method enables a baseline separation of the analytes and tropic acid, one of the typical impurities of atropine. The developed enantioseparation of atropine was performed using a commercially available sulfated beta-cyclodextrin and was validated for the determination of L-hyoscyamine in atropine as well as for the enantiomeric purity of L-hyoscyamine.
Heine
Stephanie
S
Institute of Pharmaceutical and Medicinal Chemistry, University of Münster, Hittorf-Strasse 58-62, D-48149 Münster, Germany.
Ebert
Kirstin
K
Blaschke
Gottfried
G
D001285:Atropine / Q000032:analysis / Q000302:isolation & purification*; D002138:Calibration; D003505:Cyclodextrins; D004340:Drug Contamination; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000592:standards; D010666:Phenylpropionates / Q000032:analysis / Q000302:isolation & purification; D015203:Reproducibility of Results; D013237:Stereoisomerism
D003505:Cyclodextrins; D010666:Phenylpropionates; D001285:Atropine; C011377:tropic acid