Interaction of C17orf25 with ADP-ribose pyrophosphatase NUDT9 detected via yeast two-hybrid method.
The gene C17orf25 was isolated from the liver by RACE PCR. nudt9 gene was screened by yeast two-hybrid method in MatchMaker human HeLa cDNA library. NUDT9 is an enzyme that has pyrophosphatase activity with ADP-ribose as its substrate. Fusion expression of C17orf25 and GFP and computer analysis showed that C17orf25 was probably located in mitochondria. Furthermore, C17orf25 may suppress the cell growth by interaction with NUDT9.
Zhang
Hui-Tang
HT
Research Center of Biotechnology, Shanghai Institute for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200233, China.
Yan
Zhi-Qiang
ZQ
Hu
Xiao-Bo
XB
Yang
Sheng-Li
SL
Gong
Yi
Y
D000595:Amino Acid Sequence; D015723:Gene Library; D006801:Humans; D008928:Mitochondria / Q000737:chemistry; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D016366:Open Reading Frames; D011506:Proteins / Q000235:genetics / Q000502:physiology*; D011755:Pyrophosphatases / Q000235:genetics / Q000502:physiology*; D020798:Two-Hybrid System Techniques
C452679:GLOD4 protein, human; D009363:Neoplasm Proteins; D011506:Proteins; C429463:NUDT9; D011755:Pyrophosphatases