Virus concentration using sulfonated magnetic beads to improve sensitivity in nucleic acid amplification tests.
To enhance the sensitivity of virus detection by polymerase chain reaction (PCR) and reverse-transcriptional (RT)-PCR, we developed a novel virus-concentration method using sulfonated (SO-) magnetic beads in the presence of divalent cations. In the presence of either Zn(2+) or Cu(2+) ions, we showed that SO-magnetic beads were able to concentrate non-enveloped model viruses, such as porcine parvovirus (PPV) and poliovirus, which were not concentrated by polyethyleneimine (PEI)-magnetic beads.(1)) Using the SO-magnetic beads, the sensitivity of virus genome detection by PCR or RT-PCR can be enhanced. Therefore, an efficient virus concentration method using either SO-magnetic beads or PEI-magnetic beads enhances the sensitivity of virus detection by PCR or RT-PCR.
Iwata
Akiko
A
The National Institute of Health Sciences, Tokyo, Japan.
Satoh
Koei
K
Murata
Mitsuhiro
M
Hikata
Mikio
M
Hayakawa
Takao
T
Yamaguchi
Teruhide
T
D000818:Animals; D002522:Chlorocebus aethiops; D008280:Magnetics; D008863:Microspheres; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D013451:Sulfonic Acids / Q000032:analysis*; D013552:Swine; D014709:Vero Cells; D014780:Viruses / Q000235:genetics / Q000302:isolation & purification*
D013451:Sulfonic Acids