[The effect of substance P on functional proteins in human neutrophil].
OBJECTIVE
To explore the effect of substance P (SP) on the functional proteins on plasma membrane of neutrophil (Np).


METHODS
The response of Np to SP was examined by measuring the level of respiratory burst, the activities of ACP and ALP, the fluoroscopy intensity of CR3, CD45 and FM-LP.


RESULTS
It was found that SP could increase respiratory burst of Np, decrease the activity of acid phosphatase (ACP), but had no effect on alkaline phosphatase (ALP). SP could also promote the amount of CD45, complement receptor type 3 (CR3) and N-Formyl-Met-Leu-Phe (FMLP) receptors.


CONCLUSIONS
The results showed that the effects of SP on functional proteins in human Np membrane were universality and diversity. It implied that SP could affect various inflammation responses in Np.
Yang
Lin
L
Department of Biochemistry, Institute of Hematology, CAMS, PUMC, Tianjin 300020, China.
Fa
Xiang-guang
XG
D000135:Acid Phosphatase / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000502:physiology*; D009504:Neutrophils / Q000378:metabolism*; D016897:Respiratory Burst; D013373:Substance P / Q000494:pharmacology*
D008565:Membrane Proteins; D013373:Substance P; D000135:Acid Phosphatase