[Express diagnostics of bovine leucosis by immune sensor based on surface plasmon resonance].
An immune sensor based on the surface plasmon resonance (SPR) was developed for express diagnostics of bovine leucosis. The sensor was used for detection of the level of antibodies against bovine leukaemia virus (BLV) in the blood serum. The industrially manufactured BLV antigen for screening test in the agar gel immunodiffusion (AGID) required the additional purification in order to be used in immune sensor analysis. It was shown that immune sensor analysis was more sensitive, rapid and simple in comparison with the traditional AGID test. It was stated that the developed immune sensor was capable to be used for performance of bovine leucosis screening at the farms and the minimal dilution of the serum should be 1:500.
Pyrohova
L V
LV
Palladin Institute of Biochemistry, NAS of Ukraine, Kyiv.
Starodub
M F
MF
Artiukh
V P
VP
Nahaieva
L I
LI
Dobrosol
H I
HI
D000362:Agar; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis* / Q000276:immunology; D002417:Cattle; D016583:Enzootic Bovine Leukosis / Q000175:diagnosis* / Q000276:immunology; D005779:Immunodiffusion / Q000379:methods / Q000662:veterinary; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000379:methods* / Q000662:veterinary*
D000914:Antibodies, Viral; D000362:Agar