title: Identities of two Paragonimus species from Sri Lanka inferred from molecular sequences.
abstract: Metacercariae of Paragonimus spp. were obtained from field-collected freshwater crabs in Sri Lanka. Genomic DNA was extracted from single metacercariae. Two gene regions (partial mitochondrial cytochrome c oxidase subunit 1 (CO1) and the second internal transcribed spacer of the nuclear ribosomal gene repeat (ITS2)) were amplified using the polymerase chain reaction. Two differing sequences were obtained for each of these gene regions. Phylogenetic analyses placed the type 1 sequences as sister to a clade containing P. westermani and P. siamensis whereas the type 2 sequences were close to published sequences of P. siamensis from Thailand. The possible taxonomic status of these two types are discussed. This is the first report of molecular data about Paragonimus from Sri Lanka.
authors.affiliation: Department of Environmental Health Science, Kochi Medical School, Oko, Nankoku City, Kochi 783-8505, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Iwagami
authors.forename: R P V J
authors.initials: RP
authors.lastname: Rajapakse
authors.forename: W
authors.initials: W
authors.lastname: Paranagama
authors.forename: T
authors.initials: T
authors.lastname: Agatsuma
mesh_terms: D000818:Animals; D001483:Base Sequence; D003386:Brachyura / Q000469:parasitology*; D018509:DNA, Helminth / Q000032:analysis; D003576:Electron Transport Complex IV / Q000235:genetics; D008969:Molecular Sequence Data; D010237:Paragonimiasis / Q000469:parasitology / Q000662:veterinary*; D010238:Paragonimus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010274:Parasitology / Q000379:methods; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D018509:DNA, Helminth; D003576:Electron Transport Complex IV