title: Development of novel whole-mount in situ hybridization (WISH).
abstract: We have designed a novel fluorescent PNA probe which can be rapidly introduced into the living cell without any complicated pretreatment. We could post-synthetically incorporate amino acid derivatives having membrane permeability function into the PNA probe by utilizing key compound 1 which we have already developed. We show that the PNA probe will be a candidate for a rapidly penetrating and non-degrading probe for WISH.
authors.affiliation: Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan.
authors.forename: Hisafumi
authors.initials: H
authors.lastname: Ikeda
authors.forename: Madoka
authors.initials: M
authors.lastname: Tonosaki
authors.forename: Akinori
authors.initials: A
authors.lastname: Sugiyama
authors.forename: Fumio
authors.initials: F
authors.lastname: Tashiro
authors.forename: Yushin
authors.initials: Y
authors.lastname: Nakamura
mesh_terms: D005456:Fluorescent Dyes; D017403:In Situ Hybridization / Q000379:methods*; D015335:Molecular Probes; D020135:Peptide Nucleic Acids / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D015335:Molecular Probes; D020135:Peptide Nucleic Acids