Cutting edge: the B cell chemokine CXC chemokine ligand 13/B lymphocyte chemoattractant is expressed in the high endothelial venules of lymph nodes and Peyer's patches and affects B cell trafficking across high endothelial venules.
While CCR7 ligands direct T cell trafficking into lymph nodes (LNs) and Peyer's patches (PPs), chemokines that regulate B cell trafficking across high endothelial venules (HEVs) remain to be fully elucidated. Here we report that CXC chemokine ligand (CXCL)13 (B lymphocyte chemoattractant) is detected immunohistologically in the majority of HEVs in LNs and PPs of nonimmunized mice. Systemically administered anti-CXCL13 Ab bound to the surface of approximately 50% of HEVs in LNs and PPs, but not to other types of blood vessels, indicating that CXCL13 is expressed in the HEV lumen. In CXCL13-null mice, B cells rarely adhered to PP HEVs, whereas T cells did efficiently. Superfusion of CXCL13-null PPs with CXCL13 restored the luminal presentation of CXCL13 and also B cell arrest in PP HEVs at least partially. Collectively, these results indicate that CXCL13 expressed in the HEV lumen plays a crucial role in B cell trafficking into secondary lymphoid tissues such as PPs.
Ebisuno
Yukihiko
Y
Laboratory of Molecular and Cellular Recognition, Osaka University Graduate School of Medicine, Suita, Japan.
Tanaka
Toshiyuki
T
Kanemitsu
Naotoshi
N
Kanda
Hidenobu
H
Yamaguchi
Kazuhito
K
Kaisho
Tsuneyasu
T
Akira
Shizuo
S
Miyasaka
Masayuki
M
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D002448:Cell Adhesion / Q000235:genetics / Q000276:immunology; D002465:Cell Movement / Q000235:genetics / Q000276:immunology*; D054382:Chemokine CXCL13; D019743:Chemokines, CXC / Q000096:biosynthesis* / Q000172:deficiency / Q000235:genetics / Q000502:physiology; D004729:Endothelium, Lymphatic / Q000098:blood supply* / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D008198:Lymph Nodes / Q000098:blood supply / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008856:Microscopy, Fluorescence; D010581:Peyer's Patches / Q000098:blood supply / Q000276:immunology* / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology; D014699:Venules / Q000276:immunology / Q000378:metabolism; D014743:Videotape Recording
D054382:Chemokine CXCL13; D019743:Chemokines, CXC; C486386:Cxcl13 protein, mouse