Optical heterodyne surface-plasmon resonance biosensor.
A novel optical heterodyne surface-plasmon resonance (SPR) biosensor with a Zeeman laser is proposed. Two surface plasma waves are excited by two correlated p-polarized waves in a SPR device of the Kretschmann configuration. Two reflected p waves are optically heterodyned such that the magnitude of the heterodyned signal is proportional to the multiplication of two attenuated reflected p waves. Then the detection sensitivity and the dynamic range based on this amplitude-sensitive method are enhanced. In the experiment, the kinetics between mouse immunoglobulin G (IgG) and rabbit antimouse IgG is obtained from sensograms of various concentrations of antimouse IgG. A detection sensitivity of 0.2 nM was achieved. In addition, a concentration of 5 ng/ml of protein G interacting with mouse IgG was measured successfully.
Kuo
Wen-Chuan
WC
Institute of Radiological Science and Department of Medical Radiation Technology, National Yang-Ming University, Pei-Tou, Taipei 112, Taiwan.
Chou
Chien
C
Wu
Hsieh-Ting
HT
D000818:Animals; D000906:Antibodies / Q000276:immunology; D015374:Biosensing Techniques; D007074:Immunoglobulin G / Q000276:immunology; D007700:Kinetics; D051379:Mice; D008962:Models, Theoretical; D009994:Osmolar Concentration; D011817:Rabbits; D020349:Surface Plasmon Resonance
D000906:Antibodies; D007074:Immunoglobulin G