cDNA cloning and mRNA expression of L-3,4-dihydroxyphenylalanine decarboxylase gene homologue from the silkworm, Bombyx mori.
L-3,4-Dihydroxyphenylalanine decarboxylase (DDC) cDNA, from Bombyx mori that contains an open reading frame of 1437 bp encoding 478 amino acids, was cloned and characterized. Expression analyses of B. mori DDC mRNA by Northern and in situ hybridization indicated that expression of silkworm DDC expression is possibly controlled by neuropeptide hormones in tissue- and stage-specific manners.
Hwang
Jae-Sam
JS
Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology, RDA, Suwon 441-100, South Korea. hwangjs@rda.go.kr
Kang
Seok-Woo
SW
Goo
Tae-Won
TW
Yun
Eun-Young
EY
Lee
Jin-Sung
JS
Kwon
O-Yu
OY
Chun
Taehoon
T
Suzuki
Yuriko
Y
Fujiwara
Haruhiko
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D002262:Carboxy-Lyases / Q000096:biosynthesis* / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D026461:Ecdysteroids / Q000494:pharmacology; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000235:genetics*; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein
D018076:DNA, Complementary; D026461:Ecdysteroids; D012333:RNA, Messenger; D002262:Carboxy-Lyases; C477392:L-3,4-dihydroxyphenylalanine decarboxylase, Bombyx mori