Serotonin, serotonin 5-HT(1A) receptors and dopamine in blood peripheral lymphocytes of major depression patients.
There are increasing evidences of cell markers present in the immune and the nervous systems. These include neurotransmitter receptors and transporters. Serotonin receptor subtypes are related to depression and also have been shown to be present in certain cells of the immune system. In the present report, we determined the presence of 5-HT(1A) receptors by the binding of the selective agonist 8-hydroxy-2-(di-n-propyl-amino)tetralin in lymphocytes of peripheral blood isolated by Ficoll/Hypaque gradients from controls and depressed patients. The capacity of these receptors was around 24 fmol/10(6) cells in both groups of subjects, without significant difference among them. The affinity was in the nM range and either differ between controls and patients. Serotonin, 5-hydroxyindoleacetic acid, dopamine and 3,4-dihydroxyphenylacetic acid were determined by HPLC with electrochemical detector. There were no significant differences between controls and major depression patients in the values obtained for rich and poor platelet plasma or in the isolated cells. However, there was a reduction in serotonin turnover rate indicated by an increase in the ratio serotonin/5-hydroxyindoleacetic acid, but not in that of dopamine, in lymphocytes of major depression patients. Thus, there is a serotonergic dysfunction in immune circulating cells of major depression patients, without changes in the number of 5-HT(1A) receptors, although the coupling of these receptors to transduction mechanisms could be affected and may be related to the alteration of 5-HT turnover rate.
Fajardo
O
O
Laboratorio de Neuroquímica, Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas and Centro de Salud Mental del Este, El Peñón, Caracas, Venezuela.
Galeno
J
J
Urbina
M
M
Carreira
I
I
Lima
L
L
D015102:3,4-Dihydroxyphenylacetic Acid / Q000097:blood; D017371:8-Hydroxy-2-(di-n-propylamino)tetralin / Q000494:pharmacology; D000293:Adolescent; D000328:Adult; D002851:Chromatography, High Pressure Liquid; D003866:Depressive Disorder / Q000097:blood* / Q000276:immunology; D004298:Dopamine / Q000097:blood*; D005260:Female; D006801:Humans; D006897:Hydroxyindoleacetic Acid / Q000097:blood; D008214:Lymphocytes / Q000737:chemistry*; D008297:Male; D008875:Middle Aged; D011598:Psychoneuroimmunology; D044282:Receptor, Serotonin, 5-HT1A / Q000097:blood*; D012701:Serotonin / Q000097:blood*; D017366:Serotonin Receptor Agonists / Q000494:pharmacology
D017366:Serotonin Receptor Agonists; D015102:3,4-Dihydroxyphenylacetic Acid; D044282:Receptor, Serotonin, 5-HT1A; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D017371:8-Hydroxy-2-(di-n-propylamino)tetralin; D004298:Dopamine