[Linkage disequilibrium analysis of the single nucleotide polymorphisms in the PRKCZ gene].
OBJECTIVE
To search for the disease-associated haplotype in the PRKCZ gene, a susceptibility gene for type 2 diabetes in Han population of North China, by case-control study and linkage disequilibrium (LD) analysis using single nucleotide polymorphisms (SNPs).


METHODS
SNPs located in the PRKCZ gene were chosen from public SNP domain by bioinformatic methods and single base extension (SBE) method was used to genotype the loci in 173 sporadic type 2 diabetes patients and 152 normal individuals to perform case-control study and LD analysis. Haplotype block were constructed in these populations.


RESULTS
Several SNPs in the PRKCZ gene were found to be associated with the disease. The SNPs formed different haplotype block pattern in case and control groups. The frequencies of the haplotypes formed by 5 SNPs were statistically different between the two groups.


CONCLUSIONS
The haplotype formed by 5 SNPs in the PRKCZ gene may be associated with type 2 diabetes in Han population of China, which is confirmed from statistics to be a susceptibility gene for the disease.
Sun
Hong-xia
HX
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China.
Du
Wei-nan
WN
Li
Yun-feng
YF
Zuo
Jin
J
Wu
Guo-dong
GD
Shen
Yan
Y
Qiang
Bo-qin
BQ
Yao
Zhi-jian
ZJ
Hang
Jian-mei
JM
Luo
Hui-yuan
HY
Wang
Heng
H
Huang
Wei
W
Chen
Zhu
Z
Fang
Fu-de
FD
D000483:Alleles; D044466:Asian People; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005006:Ethnicity; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D015810:Linkage Disequilibrium; D020641:Polymorphism, Single Nucleotide; D011493:Protein Kinase C / Q000235:genetics*; D051745:Protein Kinase C-delta
C497258:PRKCD protein, human; D011493:Protein Kinase C; D051745:Protein Kinase C-delta