2-Amino-6-vinyl purine as a tool for post synthetic conjugation of DNA with radio-, spin-, fluorescence labels and peptides.
We have previously demonstrated that 2-amino-6-vinylpurine is an efficient cross-linking agent with high selectivity towards cytidine. In this study, we applied this nucleobase analog to versatile conjugation of oligodeoxynucleotides with radio-, spin-, fluorescence- and peptide labels. The oligodeoxynucleotides incorporating 2-amino-6-vinylpurine nucleoside were reacted with amino- bearing nucleophies in an excess amount to give the corresponding conjugates in good yield.
Ali
Md Monsur
MM
Graduate School of Pharmaceutical Sciences, Kyushu University and CREST (JST), 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Nagatsugi
Fumi
F
Yamamoto
Fumihiko
F
Maeda
Minoru
M
Sasaki
Shigeki
S
D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D011687:Purines / Q000737:chemistry*; D011868:Radioisotopes / Q000737:chemistry*; D013113:Spin Labels; D014753:Vinyl Compounds / Q000737:chemistry*
C432203:2-amino-6-vinylpurine; D005456:Fluorescent Dyes; D010455:Peptides; D011687:Purines; D011868:Radioisotopes; D013113:Spin Labels; D014753:Vinyl Compounds; D004247:DNA