title: [The effect of overdose fluoride on the proliferation and apoptosis of rat's incisor ameloblast].
abstract: OBJECTIVE
  To observe the effect of overdose fluoride on the proliferation of rat's incisor ameloblast.
  
  
  METHODS
  20 Wistar rats were divided randomly into 2 groups: Group I (Control); Group II 50 mg/L F(-) were given. After 8 weeks treatment, the AgNORs stain and TUNEL technique were applied to analyze the effect of fluoride on the proliferation and apoptosis of ameloblasts.
  
  
  RESULTS
  The imagination analysis results showed that proliferation of pre-secretion ameloblasts were inhibited in group II as compared with the control group (P < 0.001). There was significant increase of apoptosis with the trend of migration toward secretion stage.
  
  
  CONCLUSIONS
  The mechanism of fluorosis mottled enamel may be the effect of overdose fluoride with inhibits proliferation and induces apoptosis of ameloblasts resulting in dysfunction of secretion or absorption of enamel matrix proteins.
authors.affiliation: Department of Oral Pathology, School of Stomatology, Jilin University, Changchun 130041, China.
authors.forename: Hong-chen
authors.initials: HC
authors.lastname: Sun
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Ouyang
authors.forename: Guang-sheng
authors.initials: GS
authors.lastname: Li
mesh_terms: D000565:Ameloblasts / Q000187:drug effects* / Q000473:pathology; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D003743:Dental Enamel / Q000473:pathology; D005260:Female; D005459:Fluorides / Q000009:adverse effects*; D009050:Fluorosis, Dental / Q000473:pathology*; D007180:Incisor; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005459:Fluorides