Design of an artificial restriction enzyme having simultaneous DNA cleavage activity.
We designed dimeric distamycin linked hydroxamic acid (DDHA)-metal complexes and investigated double strand simultaneous cleavage. Compared as distamycin linked hydroxamic acid (DHA)-metal complex, DDHA-metal complex cleaved DNA at the same level of DHA-metal complex without double strand simultaneous cleavage.
Inui
T
T
Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Science University of Tokyo, 2641 Yamazaki, Noda, Chiba 278-8510, Japan.
Ikeda
H
H
Nakamura
Y
Y
D004247:DNA / Q000737:chemistry / Q000187:drug effects*; D004262:DNA Restriction Enzymes; D004214:Distamycins / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D015195:Drug Design; D006877:Hydroxamic Acids / Q000737:chemistry / Q000494:pharmacology; D007501:Iron / Q000737:chemistry; D015394:Molecular Structure; D010957:Plasmids / Q000737:chemistry / Q000187:drug effects
D004214:Distamycins; D006877:Hydroxamic Acids; D004247:DNA; D007501:Iron; D004262:DNA Restriction Enzymes