title: Herd-level ELISA seroprevalence of bovine viral diarrhea antibodies in bulk-tank milk in Chilean dairy herds.
abstract: Our objective in this cross-sectional study was to determine the seroprevalence in 1996 of commercial dairy herds in central Chile positive to bovine viral diarrhea (BVD) virus, by the detection of antibodies in bulk-tank milk samples. Samples were obtained from 501 dairy farms ( approximately 50,000 cows) from the Metropolitan, VI, VII and VIII regions of Chile. An indirect ELISA was used to detect BVD antibodies. An absorbance (A450) > or = 0.55 was considered positive. The apparent proportion of positive herds ranged from 71.2 to 83% by region and did not differ by region. We concluded that exposure to BVD was widely distributed in the central area of Chile in 1996.
authors.affiliation: Department of Animal Production, Faculty of Livestock and Veterinary Sciences, University of Chile, Casilla 2, Correo 15, Santiago, Chile. melendezp@mail.vetmed.ufl.edu
authors.forename: Pedro
authors.initials: P
authors.lastname: Meléndez
authors.forename: Art
authors.initials: A
authors.lastname: Donovan
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000453:epidemiology* / Q000382:microbiology; D002417:Cattle; D002677:Chile / Q000453:epidemiology; D003430:Cross-Sectional Studies; D003612:Dairying; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D008892:Milk / Q000821:virology; D016036:Seroepidemiologic Studies
chemical_list: D000914:Antibodies, Viral