title: Detection and quantification of Mycoplasma genitalium in male patients with urethritis.
abstract: Detection and quantification of Mycoplasma genitalium were evaluated in 83 patients with urethritis (group 1), 60 patients with urethral symptoms but no urethritis (group 2), and 50 asymptomatic men (group 3). Quantification of M. genitalium was carried out using real-time polymerase chain reaction (PCR) analysis of first-pass urine samples. The rate of detection of M. genitalium was significantly higher in group 1 than in groups 2 and 3 (P<.0001). The mean observed concentration of M. genitalium was 1.2x10(4) equivalent genomes/mL of urine (range, 50 to 8x10(4) equivalent genomes/mL). Analysis of M. genitalium load in serial urine samples collected before and after the administration of antibacterial treatment showed an association between clinical and microbiological responses, with a shift to negative PCR results in symptom-free patients. Our results illustrate the usefulness of monitoring the M. genitalium load in evaluating the susceptibility of M. genitalium to antibacterial treatment.
authors.affiliation: Service de Dermato-Vénéréologie, Hôpital Cochin, Université René Descartes, Paris, France. nicolas.dupin@cch.ap-hop-paris.fr
authors.forename: Nicolas
authors.initials: N
authors.lastname: Dupin
authors.forename: Gérard
authors.initials: G
authors.lastname: Bijaoui
authors.forename: Michaël
authors.initials: M
authors.lastname: Schwarzinger
authors.forename: Pauline
authors.initials: P
authors.lastname: Ernault
authors.forename: Philippe
authors.initials: P
authors.lastname: Gerhardt
authors.forename: Randa
authors.initials: R
authors.lastname: Jdid
authors.forename: Salim
authors.initials: S
authors.lastname: Hilab
authors.forename: Christina
authors.initials: C
authors.lastname: Pantoja
authors.forename: Marc
authors.initials: M
authors.lastname: Buffet
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Escande
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Costa
mesh_terms: D004269:DNA, Bacterial / Q000032:analysis*; D006801:Humans; D008297:Male; D009174:Mycoplasma / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D009175:Mycoplasma Infections / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012749:Sexually Transmitted Diseases / Q000382:microbiology*; D014526:Urethritis / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial