title: The complete nucleotide sequence of two distinct geminiviruses infecting cucurbits in Vietnam.
abstract: We have characterised two distinct geminiviruses that infect cucurbit cultivars in Vietnam. The genomes of both viruses consisted of two circular ssDNA components (DNA-A and DNA-B), with a genome arrangement and coding sequence typical of viruses in the Begomovirus genus in the family Geminiviridae. The sequence of DNA-A of one of the viruses was approximately 97% similar to Squash leaf curl virus-China (SLCV-Ch), for which a DNA-B has yet to be identified. We have named this virus Squash leaf curl virus-Vietnam (SLCV-Vn). The intergenic region of the SLCV-Vn DNA-B contained a 40 nt deletion between the putative AC1 TATA box and the stem loop. A second virus isolated from loofa in southern Vietnam was only 80% similar to SLCV-Vn over the complete DNA-A sequence, however the nucleotide sequence in the coat protein coding regions was 95% similar. We have named this virus Loofa yellow mosaic virus-Vietnam (LYMV-Vn). Other regions of the SLCV-Vn and LYMV-Vn genomes differed markedly, suggesting the coat protein coding region was recombinant. The DNA-B of both viruses were only 60% similar over the complete nucleotide sequence, although the encoded amino acid sequence of the BC1 gene was 90% identical.
authors.affiliation: Plant Biotechnology, Science Research Centre, Queensland University of Technology, Brisbane, Australia. p.revill@qut.edu.au
authors.forename: P A
authors.initials: PA
authors.lastname: Revill
authors.forename: C V
authors.initials: CV
authors.lastname: Ha
authors.forename: S C
authors.initials: SC
authors.lastname: Porchun
authors.forename: M T
authors.initials: MT
authors.lastname: Vu
authors.forename: J L
authors.initials: JL
authors.lastname: Dale
mesh_terms: D001483:Base Sequence; D028464:Cucurbita / Q000821:virology*; D021901:DNA, Intergenic / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D017791:Geminiviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014644:Genetic Variation; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D018515:Plant Leaves / Q000821:virology; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D014744:Vietnam
chemical_list: D021901:DNA, Intergenic; D004279:DNA, Viral