Palmitoylation is required for membrane association of activated but not inactive invertebrate visual Gqalpha.
The invertebrate visual G protein, iGqalpha plays a central role in invertebrate phototransduction by relaying signals from rhodopsin to phospholipase C leading to membrane depolarization. Previous studies have shown reversible association of iGqalpha with rhabdomeric membranes regulated by light. To address the mechanism of membrane association we cloned iGqalpha from a Loligo pealei photoreceptor cDNA library and expressed it in HEK293T cells. Mutations were introduced to eliminate putative sites for palmitoylation at cysteines in positions 3 and 4. Membrane and soluble fractions were prepared from cells where iGqalpha was either activated or maintained in the GDP-bound form, followed by identification of iGqalpha through immunoblot analysis. The wild-type iGqalpha was entirely membrane-bound and shown to be post-translationally modified by palmitoylation. The mutant iGqalpha (C3,4A) was not palmitoylated yet it was found to be membrane-associated in the inactive state, however, approximately half of the protein became soluble when activated. These results suggest that palmitoylation is not required for membrane association of iGqalpha in the inactive state but is important in maintaining the stable membrane association of activated iGqalpha-GTP. The mechanism by which iGqalpha moves away from the membrane into the cytosol in response to prolonged light-stimulation in the native squid eye appears, therefore, to involve both activation and depalmitoylation processes.
Go
Lynle
L
Department of Pharmacology, University of Toronto, 1 King's College Circle, Room 4342, Toronto, ON, Canada M5S 1A8.
Mitchell
Jane
J
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D049832:Decapodiformes / Q000378:metabolism*; D043802:GTP-Binding Protein alpha Subunits, Gq-G11 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007425:Intracellular Membranes / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D017956:Photoreceptor Cells, Invertebrate / Q000378:metabolism; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D014785:Vision, Ocular / Q000502:physiology*
D021122:Protein Subunits; D043802:GTP-Binding Protein alpha Subunits, Gq-G11