[Gene clone and its characteristics on band 7-like protein in Plasmodium falciparum FCC1/HN].
OBJECTIVE
To identify and clone the gene named pfstom gene which encoding the protein belonging to band 7 family and to do primary research on its function.


METHODS
Based on the finished data in international public malaria database, coding sequence of pfstom cDNA was obtained by RT-PCR from FCC1/HN. Its phylogenetic profiles and the homogeny were analyzed by some softwares. After Prokaryotic expression, C terminal of Pfstom protein was expressed by Pet30a system. Recombinant Pfstom protein was used to immol/Lunize rabbit and then serum was harvested and the IgG was purified for Western blot.


RESULTS
The coding sequence of pfstom is 1,125 bp which encoding 374 amino acids with C-terminal fragment being homogenous to stomatin-like protein which belongs to band 7 family. Phylogenetic profiles analysis revealed its homogeny to stomatin. Western blot showed its stage-specific expression in trophozoite.


CONCLUSIONS
Pfstom belongs to band-7 family. It was expressed specifically in trophozoite in erythrocyte stage of plasmodium falciparum. It was not expressed in ring stage. And it is membrane-related protein. All these results provided the foundation for further research on pfstom.
Zhang
Ling
L
Etiology Department, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China.
Zhang
Lian-hui
LH
Wang
Hai-yi
HY
Wang
Heng
H
D000595:Amino Acid Sequence; D000818:Animals; D001798:Blood Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D018076:DNA, Complementary / Q000235:genetics; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D010802:Phylogeny; D010963:Plasmodium falciparum / Q000737:chemistry / Q000235:genetics* / Q000472:pathogenicity; D015800:Protozoan Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D011817:Rabbits; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
D001798:Blood Proteins; D018076:DNA, Complementary; D008565:Membrane Proteins; D015800:Protozoan Proteins; D011994:Recombinant Proteins