Atomic force microscopy study of the interaction of Fusarium solani pisi cutinase with lipid surfaces.
We present an atomic force microscopy (AFM) study of a supported triacylglyceride multilayer phase and its interaction with lipolytic enzyme cutinase from Fusarium solani pisi. The multilayer triacylglyceride phase of coconut oil showed a rippled surface structure in the AFM images. Upon enzymatic degradation of the triacylglyceride phase, the ripple structure vanished rapidly. The apparent catalytic rate constants could be estimated based on the AFM image information. Interestingly, in one sample we observed what we interpret as a recurrent structural collapse of the cavity dug out by the protein. We interpret the cavities seen in the AFM images as molten surfaces or surface holes filled with liquidified phase containing product molecules, which appear transparent during the image recording.
Fojan
P
P
Department of Life Sciences, Biostructure and Protein Engineering Group, Aalborg University, Sohngaardsholmsvej 49, DK9000 Aalborg, Denmark.
Rasmussen
T
T
Sunesen
C Ø
CØ
Petersen
S B
SB
D001665:Binding Sites; D002152:Calorimetry, Differential Scanning; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry*; D000074263:Coconut Oil; D004789:Enzyme Activation; D004795:Enzyme Stability; D005670:Fusarium / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry; D046911:Macromolecular Substances; D008422:Materials Testing / Q000379:methods*; D018625:Microscopy, Atomic Force / Q000379:methods*; D036103:Nanotechnology / Q000379:methods*; D010938:Plant Oils / Q000737:chemistry*; D011485:Protein Binding; D015183:Restriction Mapping; D012680:Sensitivity and Specificity; D013379:Substrate Specificity; D013696:Temperature
D008055:Lipids; D046911:Macromolecular Substances; D010938:Plant Oils; D002265:Carboxylic Ester Hydrolases; C019952:cutinase; D000074263:Coconut Oil