[Expression of proteolytic enzymes of metastatic tumor cells and the effects of some influencing factors].
OBJECTIVE
To elucidate the types of proteolytic enzymes the dendritic cell sarcoma (DCS) cell expressed in vitro and the effects of modulating factors.


METHODS
The proteolytic spots of DCS cells were examined, with ten different proteinase inhibitors, various antibodies and matrix.


RESULTS
Aprotinin, EDTA-Na2 and pepstatin could inhibit the proteolysis of DCS cells respectively. Anti-ubiquitin antibody and anti-DCS antibody (1:200-1:100) showed no obvious inhibitory effects. DCS cells spread out fairly well but with no proteolysis.


CONCLUSIONS
Under the conditions of our experiments the DCS cells had serine proteinase, metalloproteinase and aspartate proteinase activities. Antiubiquitin antibodies and anti-DCS antibodies showed no obvious influence on the proteolysis of DCS cells. DCS cells displayed different proteolysis status on different matrix.
Liu
Y
Y
Department of Pathology, Institute of Basic Medical Science, CAMS, PUMC, Beijing 100005, China. liuyuqin@public.bta.net.cn
Gu
P
P
Gao
J
J
Duan
D
D
Zhao
X
X
D016282:Aspartic Acid Endopeptidases / Q000378:metabolism; D003713:Dendritic Cells / Q000201:enzymology*; D016326:Extracellular Matrix Proteins / Q000494:pharmacology*; D045726:Metalloproteases / Q000378:metabolism; D009362:Neoplasm Metastasis; D010447:Peptide Hydrolases / Q000378:metabolism*; D011480:Protease Inhibitors / Q000494:pharmacology*; D012509:Sarcoma / Q000201:enzymology* / Q000473:pathology; D012697:Serine Endopeptidases / Q000378:metabolism; D014407:Tumor Cells, Cultured
D016326:Extracellular Matrix Proteins; D011480:Protease Inhibitors; D045726:Metalloproteases; D010447:Peptide Hydrolases; D012697:Serine Endopeptidases; D016282:Aspartic Acid Endopeptidases