title: ESTWeb: bioinformatics services for EST sequencing projects.
abstract: ESTWeb is an internet based software package designed for uniform data processing and storage for large-scale EST sequencing projects. The package provides for: (a) reception of sequencing chromatograms; (b) sequence processing such as base-calling, vector screening, comparison with public databases; (c) storage of data and analysis in a relational database, (d) generation of a graphical report of individual sequence quality; and (e) issuing of reports with statistics of productivity and redundancy. The software facilitates real-time monitoring and evaluation of EST sequence acquisition progress along an EST sequencing project.
authors.affiliation: Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, 05508-900, São Paulo, SP, Brazil.
authors.forename: Apuã C M
authors.initials: AC
authors.lastname: Paquola
authors.forename: Milton Y
authors.initials: MY
authors.lastname: Nishyiama
authors.forename: Eduardo M
authors.initials: EM
authors.lastname: Reis
authors.forename: Aline M
authors.initials: AM
authors.lastname: da Silva
authors.forename: Sergio
authors.initials: S
authors.lastname: Verjovski-Almeida
mesh_terms: D019295:Computational Biology / Q000379:methods; D003628:Database Management Systems; D004282:Documentation; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling / Q000379:methods*; D016247:Information Storage and Retrieval / Q000379:methods; D020407:Internet; D016415:Sequence Alignment / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*; D012984:Software; D014584:User-Computer Interface