Statistical analysis of intramembranous particles using freeze fracture specimens.
We studied the point processes of intramembranous particles of mitochondrial membranes from HeLa cells using the freeze fracture technique. Three groups - under normal conditions, after exposition with rotenone, and after exposition with sodium acid - were compared. First, we used several summary statistics in order to study the two-dimensional point patterns of intramembranous particles within each group. Then, we compared the patterns in different groups by bootstrap tests using the K-function and the nearest neighbour distance function G(r). Estimation of the G-function provided significant results but no significant differences between groups were found using the classical K-function; estimation of G(r) should therefore not be omitted when studying observed planar point patterns.
Schladitz
Katja
K
Fraunhofer ITWM, Kaiserslautern, Germany.
Särkkä
Aila
A
Pavenstädt
Iris
I
Haferkamp
Otto
O
Mattfeldt
Torsten
T
D003627:Data Interpretation, Statistical; D005614:Freeze Fracturing / Q000379:methods*; D006367:HeLa Cells / Q000648:ultrastructure; D006801:Humans; D007425:Intracellular Membranes / Q000648:ultrastructure*; D008565:Membrane Proteins / Q000378:metabolism*; D008854:Microscopy, Electron; D008928:Mitochondria / Q000648:ultrastructure*; D013269:Stochastic Processes
D008565:Membrane Proteins