[Detection of anti-HEV IgG and analysis of partial HEV RNA sequence].
OBJECTIVE
To investigate HEV infection in swine and the genotype relationship between swine and human HEV.


METHODS
Anti-HEV IgG antibody was detected in the sera of swine using enzyme linked immunoassay (EIA), and HEV RNA was amplified by reverse transcription nested polymerase chain reaction (RT-nPCR). The Vector NTI Suite 7 and TreeView softwares were used for nucleotide sequences phylogenetic analysis of HEV isolated from human and swine.


RESULTS
The anti-HEV IgG positive rate was 16.67% (18/108). Among the 18 anti-HEV IgG positive sera, 2 sequences (11.11%, called S18 and S43, respectively) of HEV ORF1 (102-387bp) were amplified, with the identity of 99% between them. They had 76% to 77%, 78%, 76% to 79%, 85% to 86%, 77%, 80%, 79% and 75% - 79% homology at the nucleotide level with human HEV genotypes 1 to 8, respectively. One (S18) of them was also amplified out in ORF2 region (5,994-6 297bp) and showed 76% to 78%, 74%, 74% to 77%, and 85% to 94% identity with human HEV genotypes 1 to 4 at the nucleotide level, respectively.


CONCLUSIONS
HEV sequences isolated from swine belong to human HEV genotype 4.
Zhu
Yong-hong
YH
Department of Microbiology and Parasitology, Health Science Center, Peking University, Beijing 100083, China.
Zhuang
Hui
H
Dong
Qing-ming
QM
Chen
Yan-feng
YF
Li
Zheng-tai
ZT
Wu
Hua
H
Liu
Jian
J
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D001483:Base Sequence; D016751:Hepatitis E / Q000635:transmission / Q000662:veterinary*; D016752:Hepatitis E virus / Q000145:classification / Q000235:genetics*; D007074:Immunoglobulin G / Q000097:blood*; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D013553:Swine Diseases / Q000821:virology*
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D012367:RNA, Viral