[Experiment study of c-fos expression on myocardial acute ischemia/reperfusion injury in rats].
OBJECTIVE
To investigate the changes of c-fos mRNA induced by myocardial ischemia/reperfusion(I/R) during acute period.


METHODS
The model of I/R was established, and the rats was divided normal control, ischmia and I/R groups. Insitu hybridization (ISH) and computerized image analysis method was used to observe alteration of c-fos mRNA in cardiac myocytes.


RESULTS
After 10 min ischemia and 30 min reperfusion, the area of reperfusion showed a part of cardiac myocytes weak staining, and get to peak at I-60 min/R-30 min. The myocardium in normal control and ischemia groups less than 60 min showed negative staining. No changes were found in all groups by HE staining.


CONCLUSIONS
c-fos mRNA detection may become an important method for diagnosis of I/R.
Ma
Meng-yun
MY
Shenzhen Criminal Science & Technology Institute, Shenzhen 518008, China.
Xu
Xiaohu
X
D000818:Animals; D005260:Female; D005554:Forensic Medicine; D017403:In Situ Hybridization; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000235:genetics* / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger