Study on the anti-tumor efficacy induced by heat shock protein 70-peptide complexes derived from tumor cells.
OBJECTIVE
To study the efficacy and explore the mechanism of the anti-tumor immunity elicited by heat shock protein 70-peptide complexes (HSP70-PC) derived from tumor cells.


METHODS
Cells culture, flow cytometric analysis, affinity chromatography for protein purification, SDS-PAGE, Western-blotting and animal experiment were used.


RESULTS
HSP70-PC immunization rendered protective effect to both naive tumorl-bearing mice. All of the naive mice obtained complete resistance to Hcaf cell attack; 40% of the tumor-bearing mice survived for over 90 days, whereas the mice of control group died within 2 weeks (P < 0.01). CD8+ subset of T lymphocytes in the peripheral blood of immunized mice increased by 12%.


CONCLUSIONS
HSP70-PC induces anti-tumor immunity via activation of cytotoxic T lymphocytes (CTLs), and it possesses strong tumor vaccine effect. Our research adds more evidence to support the clinical use of HSP70-PC to fight human cancers.
Fu
Qingguo
Q
The Second General Surgery Department, the First Affiliated Hospital of China Medical University, Shenyang 110001.
Zhang
Wei
W
Meng
Fandong
F
Guo
Renxuan
R
Yao
Zhenyu
Z
D000818:Animals; D016827:CD8 Antigens / Q000097:blood; D002462:Cell Membrane / Q000737:chemistry; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000627:therapeutic use*; D008114:Liver Neoplasms, Experimental / Q000737:chemistry / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D051379:Mice; D009368:Neoplasm Transplantation; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014407:Tumor Cells, Cultured
D016827:CD8 Antigens; D018840:HSP70 Heat-Shock Proteins