Identification of second and third calcitonin receptor-stimulating peptides in porcine brain.
We identified two cDNAs encoding new calcitonin receptor-stimulating peptides (CRSPs) in porcine hypothalamus cDNA library by cross-hybridization with the CRSP cDNA, and designated the second and third peptides as CRSP-2 and CRSP-3. The putative amino acid sequences of prepro-CRSP-2 and prepro-CRSP-3 showed higher identity with that of prepro-CRSP-1 than that of prepro-calcitonin gene-related peptide (CGRP), respectively, and these three CRSPs are considered to form a new family in the CGRP superfamily. RT-PCR analysis demonstrated that both CRSP-2 and CRSP-3 gene transcripts were expressed mainly in the central nervous system and thyroid gland. Synthetic CRSP-2 and CRSP-3 stimulated cAMP production very weakly in LLC-PK(1) cells compared with CRSP and calcitonin (CT). Furthermore, CRSP-2 and CRSP-3 did not elicit a cAMP elevation at all in the COS-7 cells expressing CT receptor or CT-like receptor with or without one of receptor activity-modifying proteins. These results suggest the presence of still unidentified action mechanisms and functions of the peptides in the CGRP superfamily.
Katafuchi
Takeshi
T
National Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Suita 565-8565, Osaka, Japan.
Hamano
Kazumasa
K
Kikumoto
Katsuro
K
Minamino
Naoto
N
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D019556:COS Cells; D015740:Calcitonin Gene-Related Peptide / Q000145:classification*; D002460:Cell Line; D000242:Cyclic AMP / Q000096:biosynthesis; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D018003:Receptors, Calcitonin / Q000378:metabolism; D018015:Receptors, Calcitonin Gene-Related Peptide / Q000378:metabolism; D013552:Swine; D014018:Tissue Distribution
D010455:Peptides; D012333:RNA, Messenger; D018003:Receptors, Calcitonin; D018015:Receptors, Calcitonin Gene-Related Peptide; D000242:Cyclic AMP; D015740:Calcitonin Gene-Related Peptide