title: Genomovar diversity amongst Burkholderia cepacia complex isolates from an Australian adult cystic fibrosis unit.
abstract: In this study, a combination of recA-based PCR assays and 16S rDNA restriction fragment length polymorphism (RFLP) analysis was used to determine the genomovar diversity of clinical Burkholderia cepacia complex isolates. Twenty-eight isolates were prospectively collected from patients attending a large Australian adult cystic fibrosis (CF) unit, 22 isolates were referred from other Australian CF units and a further eight isolates originated from patients without CF. The 28 prospectively collected isolates were distributed amongst the following genomovars: Burkholderia cepacia genomovar I (28.6%), Burkholderia multivorans (21.4%), Burkholderia cepacia genomovar III (39.3%), Burkholderia vietnamiensis(3.6%) and Burkholderia ambifaria (7.1%). The results of this study highlight the usefulness of 16S rDNA RFLP typing for the identification of other Burkholderia spp. and non-fermenting gram-negative bacteria.
authors.affiliation: Department of Microbiology, Queensland Health Pathology Service, The Prince Charles Hospital, Rode Road, Chermside, Brisbane, 4032 Queensland, Australia. Tim_Kidd@health.qld.gov.au
authors.forename: T J
authors.initials: TJ
authors.lastname: Kidd
authors.forename: S C
authors.initials: SC
authors.lastname: Bell
authors.forename: C
authors.initials: C
authors.lastname: Coulter
mesh_terms: D000328:Adult; D000818:Animals; D001315:Australia / Q000453:epidemiology; D019121:Burkholderia Infections / Q000382:microbiology; D016956:Burkholderia cepacia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D003550:Cystic Fibrosis / Q000150:complications / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D014644:Genetic Variation; D006801:Humans; D016133:Polymerase Chain Reaction; D011446:Prospective Studies; D012141:Respiratory Tract Infections / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial