title: Evaluation of the Merlin, Micronaut system for automated antimicrobial susceptibility testing of Pseudomonas aeruginosa and Burkholderia species isolated from cystic fibrosis patients.
abstract: Since accurate antimicrobial susceptibility testing of bacterial cystic fibrosis isolates is known to be problematic and an optimal in vitro testing method has not yet been evaluated, the study presented here was conducted to compare the performance of the reference agar dilution method and broth microdilution with a commercially available automated susceptibility test system (Merlin; Micronaut, Germany). In this pilot study, the susceptibility of 70 clinical strains of Pseudomonas aeruginosa and Burkholderia cepacia-like organisms to nine antimicrobial agents was tested using these methods. Susceptibility results generated by broth microdilution (both automated and according to the National Committee for Clinical Laboratory Standards recommendations) were demonstrated to be of good reproducibility, and they compared favourably to the time- and material-consuming standard agar dilution reference method, especially after a prolonged incubation time (48 h).
authors.affiliation: Institut für Medizinische Mikrobiologie, Medizinische Hochschule Hannover, Carl-Neuberg-Strasse 1, 30625, Hannover, Germany. susanne.haeussler@gbf.de
authors.forename: S
authors.initials: S
authors.lastname: Häussler
authors.forename: S
authors.initials: S
authors.lastname: Ziesing
authors.forename: G
authors.initials: G
authors.lastname: Rademacher
authors.forename: L
authors.initials: L
authors.lastname: Hoy
authors.forename: H
authors.initials: H
authors.lastname: Weissbrodt
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001331:Automation; D001431:Bacteriological Techniques; D019117:Burkholderia / Q000187:drug effects / Q000302:isolation & purification*; D003550:Cystic Fibrosis / Q000188:drug therapy / Q000382:microbiology*; D024881:Drug Resistance, Bacterial; D005260:Female; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D011552:Pseudomonas Infections / Q000175:diagnosis / Q000188:drug therapy; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
chemical_list: D000900:Anti-Bacterial Agents