Technicolour transgenics: imaging tools for functional genomics in the mouse.
Over the past decade, a battery of powerful tools that encompass forward and reverse genetic approaches have been developed to dissect the molecular and cellular processes that regulate development and disease. The advent of genetically-encoded fluorescent proteins that are expressed in wild type and mutant mice, together with advances in imaging technology, make it possible to study these biological processes in many dimensions. Importantly, these technologies allow direct visual access to complex events as they happen in their native environment, which provides greater insights into mammalian biology than ever before.
Hadjantonakis
Anna-Katerina
AK
Department of Genetics and Development, College of Physicians and Surgeons of Columbia University, New York 10032, USA.
Dickinson
Mary E
ME
Fraser
Scott E
SE
Papaioannou
Virginia E
VE
D000818:Animals; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D007668:Kidney / Q000196:embryology; D008164:Luminescent Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence / Q000379:methods*; D016296:Mutagenesis; D013997:Time Factors
D008164:Luminescent Proteins; C413663:fluorescent protein 583; D049452:Green Fluorescent Proteins