title: Trans-acting regulatory variation in Saccharomyces cerevisiae and the role of transcription factors.
abstract: Natural genetic variation can cause significant differences in gene expression, but little is known about the polymorphisms that affect gene regulation. We analyzed regulatory variation in a cross between laboratory and wild strains of Saccharomyces cerevisiae. Clustering and linkage analysis defined groups of coregulated genes and the loci involved in their regulation. Most expression differences mapped to trans-acting loci. Positional cloning and functional assays showed that polymorphisms in GPA1 and AMN1 affect expression of genes involved in pheromone response and daughter cell separation, respectively. We also asked whether particular classes of genes were more likely to contain trans-regulatory polymorphisms. Notably, transcription factors showed no enrichment, and trans-regulatory variation seems to be broadly dispersed across classes of genes with different molecular functions.
authors.affiliation: Howard Hughes Medical Institute and Division of Human Biology, Fred Hutchinson Cancer Research Center, Mailstop D4-100, 1100 Fairview Avenue North, Seattle, Washington 98103 USA.
authors.forename: Gaël
authors.initials: G
authors.lastname: Yvert
authors.forename: Rachel B
authors.initials: RB
authors.lastname: Brem
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Whittle
authors.forename: Joshua M
authors.initials: JM
authors.lastname: Akey
authors.forename: Eric
authors.initials: E
authors.lastname: Foss
authors.forename: Erin N
authors.initials: EN
authors.lastname: Smith
authors.forename: Rachel
authors.initials: R
authors.lastname: Mackelprang
authors.forename: Leonid
authors.initials: L
authors.lastname: Kruglyak
mesh_terms: D000595:Amino Acid Sequence; D018797:Cell Cycle Proteins / Q000502:physiology; D003001:Cloning, Molecular; D015966:Gene Expression Regulation, Fungal; D008040:Genetic Linkage; D014644:Genetic Variation; D008969:Molecular Sequence Data; D005810:Multigene Family; D012045:Regulatory Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000502:physiology; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: C473476:Amn1 protein, S cerevisiae; D018797:Cell Cycle Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors