Production of galactinol from sucrose by plant enzymes.
Galactinol, 1-O-(alpha-D-galactopyranosyl)-myo-inositol, was produced from sucrose as a starting material. UDP-Glc was prepared with sucrose and UDP using sucrose synthase partially purified from sweet potato roots. Then, the UDP-Glc was converted to UDP-Gal using yeast UDP-Gal 4-epimerase from a commercial source. Finally, galactinol was produced from the UDP-Gal and myo-inositol using galactinol synthase partially purified from cucumber leaves. The product was identified as galactinol by the retention times of HPLC, alpha-galactosidase digestion, and NMR spectrometry.
Wakiuchi
Nariaki
N
The Graduate School of Science and Technology, Kobe University, Nada, Japan. wakiuchi@kobe-u.ac.jp
Shiomi
Ryohei
R
Tamaki
Hajime
H
D002851:Chromatography, High Pressure Liquid; D018553:Cucumis sativus / Q000201:enzymology*; D004187:Disaccharides / Q000096:biosynthesis*; D005700:Galactosyltransferases / Q000037:antagonists & inhibitors / Q000378:metabolism; D005964:Glucosyltransferases / Q000378:metabolism; D027723:Ipomoea batatas / Q000201:enzymology*; D009682:Magnetic Resonance Spectroscopy; D013395:Sucrose / Q000378:metabolism*
D004187:Disaccharides; C013536:6 beta-galactinol; D013395:Sucrose; D005700:Galactosyltransferases; D005964:Glucosyltransferases; C096645:inositol 1-alpha-galactosyltransferase; C022699:sucrose synthase