[Eukaryotic expression and in vitro tumor cell apoptosis-induced effect of human TRAIL].
OBJECTIVE
To explore the tumor cell apoptosis induced by the effect in vitro of eukaryotic expression of full-length TRAIL cDNA and its extracellular region.


METHODS
The eukaryotic expression vectors for both forms of the cDNA acquired from the fetal heart cDNA library were constructed. After gene transfecting, the stable expression cell lines were obtained by G418 screening.


RESULTS
The supernatants from tansfectants could induce apoptosis of different tumor cell lines in vitro, and an enhanced effect was observed by adding TFAR19 (TF-1 cell apoptosis-related protein 19), a novel apoptosis gene product discovered in our laboratory.


CONCLUSIONS
Eukaryotic expression products of TRAIL can induce apoptosis of the tumor cells, and TFAR19 could enhance the effect on apoptosis of tumor cells.
Shi
X
X
Molecular Immunology Laboratory, Peking University, Medical Immunology Key Laboratory of Health Ministry of China, Peking University Center for Human Disease Genomics, Beijing 100083, China. xu.shi@mailcity.com
Huang
J Q
JQ
Zhang
Y M
YM
Song
Q S
QS
Ma
D L
DL
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins; D018076:DNA, Complementary / Q000235:genetics*; D004357:Drug Synergism; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D051379:Mice; D041681:NIH 3T3 Cells; D009363:Neoplasm Proteins / Q000494:pharmacology; D053221:TNF-Related Apoptosis-Inducing Ligand; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology
D051017:Apoptosis Regulatory Proteins; D018076:DNA, Complementary; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; C117283:PDCD5 protein, human; C486488:Pdcd5 protein, mouse; D053221:TNF-Related Apoptosis-Inducing Ligand; C505653:TNFSF10 protein, human; C505654:Tnfsf10 protein, mouse; D014409:Tumor Necrosis Factor-alpha