Rapid identification of Candida glabrata with a new commercial test, GLABRATA RTT.
The GLABRATA RTT test (Fumouze Diagnostics, Levallois Perret, France) is based on the ability of Candida glabrata to hydrolyze trehalose but not maltose. It requires an inoculum of only four to six colonies, and the results are available within 20 min. We tested GLABRATA RTT with 330 stock isolates grown in subcultures on four different primary fungal isolation media and obtained a sensitivity of 94 to 98% (depending on the medium used) and a specificity of 97.3 to 98.6%. The false-positive results corresponded to C. tropicalis, C. famata, and C. lusitaniae. GLABRATA RTT thus offers rapid and reliable identification of C. glabrata.
Freydiere
A-M
AM
Laboratoire de Bacteriologie, Hôpital Debrousse, Lyon, France. am.freydiere@chu-lyon.fr
Robert
R
R
Ploton
C
C
Marot-Leblond
A
A
Monerau
F
F
Vandenesch
F
F
D002175:Candida / Q000145:classification; D041221:Candida glabrata / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism; D005189:False Positive Reactions; D006801:Humans; D008320:Maltose / Q000378:metabolism; D009172:Mycology / Q000295:instrumentation / Q000379:methods; D012680:Sensitivity and Specificity; D014199:Trehalose / Q000378:metabolism
D008320:Maltose; D014199:Trehalose