title: [Hemangioblastic characteristics of fetal bone marrow-derived Flk1+ CD34- cells].
abstract: OBJECTIVE
  To investigate whether fetal bone marrow stromal cells have hemangioblastic characteristics.
  
  
  METHODS
  Human fetal bone marrow stromal cells (hfMSCs) were isolated and cultured. Immunophenotypes of hfMSCs were tested by FACS. hfMSCs seeded in the matrigel were induced with vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in vitro. Vascularization and hematopoiesis were detected with immunohistochemistry and electron microscope.
  
  
  RESULTS
  The typical properties of this CD34- stromal cell population were that 99% cells expressed Flk1 (vascular endothelial cell growth factor receptor 2) and tube structure was formed. In the process of induction, hfMSCs could give rise to CD34+ round cells.
  
  
  CONCLUSIONS
  We have demonstrated that fetal bone marrow stroma-derived Flk1+ CD34- cells could differentiate into vascular endothelial cells and hematopoietic cells, indicating that fetal bone marrow stroma-derived Flk1+ CD34- cells have hemangioblastic characteristics.
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology, CAMS, PUMC, Tianjin 300020, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Guo
authors.forename: Zhi-gang
authors.initials: ZG
authors.lastname: Zhao
authors.forename: Jie-wen
authors.initials: JW
authors.lastname: Liu
authors.forename: Hui-shu
authors.initials: HS
authors.lastname: Chen
authors.forename: Chun-hua
authors.initials: CH
authors.lastname: Zhao
mesh_terms: D018952:Antigens, CD34 / Q000276:immunology*; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D005333:Fetus; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D006801:Humans; D016130:Immunophenotyping; D017154:Stromal Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000502:physiology*
chemical_list: D018952:Antigens, CD34; D016222:Fibroblast Growth Factor 2; D040301:Vascular Endothelial Growth Factor Receptor-2