title: Stable rAAV-mediated transduction of rod and cone photoreceptors in the canine retina.
abstract: Recombinant adeno-associated virus (rAAV) vectors are attractive candidates for the treatment of inherited and acquired retinal disease. Although rAAV vectors are well characterized in rodent models, a prerequisite to their clinical application in human patients is the thorough evaluation of their efficacy and safety in intermediate animal models. In this study, we describe rAAV-2-mediated expression of GFP reporter gene in retinal cells following local vector delivery in dogs. Subretinal delivery of rAAV.CMV.GFP was performed unilaterally in eight normal dogs from 6 weeks of age. The area of retinal transduction was maximized by the optimization of surgical techniques for subretinal vector delivery by pars-plana vitrectomy and the use of fine-gauge subretinal cannulae to create multiple retinotomies. rAAV-2 vectors mediated efficient stable reporter gene expression in photoreceptors and retinal pigment epithelial cells. We found efficient transduction of cone photoreceptors in addition to rods in both the canine retina and after subretinal vector delivery in another intermediate animal model, the feline retina. GFP expression in dogs was confined to the area of the retinal bleb and was sustained in cells at this site for at least 18 months. Electroretinography demonstrated a modest reduction in global rod-mediated retinal function following subretinal delivery of rAAV.CMV.GFP. Three of the eight animals developed delayed-onset intraocular inflammation, in two cases associated with a serum antibody response to GFP protein. We conclude that rAAV-2 vectors mediate efficient sustained transgene expression in rod and cone photoreceptors following subretinal delivery in this intermediate animal model. The possibility of adverse effects including intraocular immune responses and reduced retinal function requires further investigation prior to clinical applications in patients.
authors.affiliation: Department of Molecular Genetics, Institute of Ophthalmology, University College London, London, UK.
authors.forename: J W B
authors.initials: JW
authors.lastname: Bainbridge
authors.forename: A
authors.initials: A
authors.lastname: Mistry
authors.forename: F C
authors.initials: FC
authors.lastname: Schlichtenbrede
authors.forename: A
authors.initials: A
authors.lastname: Smith
authors.forename: C
authors.initials: C
authors.lastname: Broderick
authors.forename: M
authors.initials: M
authors.lastname: De Alwis
authors.forename: A
authors.initials: A
authors.lastname: Georgiadis
authors.forename: P M
authors.initials: PM
authors.lastname: Taylor
authors.forename: M
authors.initials: M
authors.lastname: Squires
authors.forename: C
authors.initials: C
authors.lastname: Sethi
authors.forename: D
authors.initials: D
authors.lastname: Charteris
authors.forename: A J
authors.initials: AJ
authors.lastname: Thrasher
authors.forename: D
authors.initials: D
authors.lastname: Sargan
authors.forename: R R
authors.initials: RR
authors.lastname: Ali
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D000229:Dependovirus / Q000235:genetics*; D004285:Dogs; D004596:Electroretinography; D005654:Fundus Oculi; D015870:Gene Expression; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000008:administration & dosage*; D049452:Green Fluorescent Proteins; D007249:Inflammation; D008164:Luminescent Proteins / Q000235:genetics; D020419:Photoreceptor Cells, Vertebrate / Q000276:immunology / Q000378:metabolism* / Q000502:physiology; D010857:Pigment Epithelium of Eye / Q000378:metabolism; D012164:Retinal Diseases / Q000628:therapy*; D013997:Time Factors; D014161:Transduction, Genetic / Q000379:methods*
chemical_list: D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins