The study on related genes in the neoplastic transformation of immortalized human fetal tracheal fibroblast cells induced by irradiation.
In this study, we investigated the genes related to the transformation of immortalized human fetal tracheal fibroblast cell line induced by alpha particles by means of differential display mRNA method. The result revealed that there were 23 DNA fragments that were expressed intensively in alphaSHTF cells (SHTF cells forming clone on agar after irradiated by alpha particles emitted by 238Pu) only and not in SHTF (SV40-immortalized human fetal tracheal fibroblast) cells. Northern dot confirmed two fragments, C17-5, C23-1 which showed intensive mRNA expression in alphaSHTF cells, but not in SHTF cells. The length of the C17-5 fragment was 310bp. Searching in BLAST database revealed that the C17-5 fragment might be an unknown sequence.
Yang
M
M
Institute of Radiation Medicine, Beijing 100850.
Liu
L
L
Ye
C
C
Wu
D
D
D001483:Base Sequence; D002460:Cell Line; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D053938:DNA Fragmentation; D004273:DNA, Neoplasm / Q000235:genetics*; D005333:Fetus; D005347:Fibroblasts / Q000378:metabolism / Q000528:radiation effects*; D020869:Gene Expression Profiling; D006801:Humans; D008969:Molecular Sequence Data; D011005:Plutonium; D012333:RNA, Messenger / Q000096:biosynthesis*; D014132:Trachea / Q000166:cytology / Q000528:radiation effects*
D004273:DNA, Neoplasm; D012333:RNA, Messenger; C026885:plutonium dioxide; D011005:Plutonium