Effect of Zn7-metallothionein on oxidative stress in liver of rats with severe thermal injury.
OBJECTIVE
Using the model of burned animal with delayed resuscitation to study antagonistic effect of Zn7-metallothionein (Zn7-MT) on oxidative stress in the liver of rats suffered from severe thermal injury on skin.


METHODS
To compare the changes in antioxidant concentrations and antioxidative enzyme activities in the liver or plasma of burned rats with or without Zn7-MT in resuscitation fluid by biochemical assay.


RESULTS
After injury, glutathione concentration was progressively decreased with time. At 24 h after injury, activities of glutathione reductase and glutathione peroxidase in the liver of burned rats were increased and then decreased at 48 h postburn. Alpha-tocopherol in plasma was reduced at 24 h and malondialdehyde in the liver was increased significantly postburn. MT and MT-1 mRNA expression in burned rats were activated. Taken together, oxidative stress in the liver of burned rats occurred. Exogenous Zn7-MT attenuated the changes in antioxidant concentrations and antioxidative enzyme activities in the liver or plasma of burned rats. The effect of Zn7-MT was in a concentration-dependent manner and the concentration of 10 micromol/L was the most effective. Exogenous Zn7-MT also inhibited MT-1 mRNA overexpression and increased MT protein concentration.


CONCLUSIONS
Zn7-MT effectively antagonized oxidative stress in the liver of rats with severe thermal injury.
Zhou
Zhi-Bo
ZB
Department of Biophysics, Health Science Center, Peking University, Beijing 100083, China.
Ding
Hai-Qin
HQ
Qin
Feng-Jun
FJ
Liu
Lin
L
Cheng
Shi
S
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002056:Burns / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008668:Metallothionein / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015032:Zinc / Q000097:blood / Q000378:metabolism; D024502:alpha-Tocopherol / Q000097:blood
D000975:Antioxidants; C021267:zinc thionein; D008315:Malondialdehyde; D008668:Metallothionein; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005978:Glutathione; D024502:alpha-Tocopherol; D015032:Zinc