[Experimental study of tissue engineered blood vessel with vascular endothelial cell and vascular smooth muscle cell].
OBJECTIVE
To investigate the feasibility to seed vascular endothelial cell(VEC) and vascular smooth muscle cell (VSMC) into tissue engineered blood vessel scaffold material.


METHODS
1. A blood vessel scaffold with a combined polymer was designed, which mainly is composed of rabbit VSMC and collagen with reinforcement by a non-spinning fabric mesh made of polyglycolic acid (PGA). 2. VEC were isolated from rabbit thoracic aorta by enzyme digestion methods and subcultured and purified. Then the cells were seeded into scaffold material. The morphological characteristics of tissue engineered blood vessel was analyzed by scanning electron microscopy.


RESULTS
VEC could adhere well to the inner surface of the tissue engineered tubular scaffold material with a tenacity and elasticity. VSMC could sustain bioactivity of cell.


CONCLUSIONS
Non-spinning PGA porous biodegradable materials coated with collagen is benefit for cells to adhere and grow. It will lay a foundation of a laminated structure of tissue engineered blood vessel.
Pan
Yong
Y
Plastic Surgery Center, Xijing Hospital, 4th Military Medical University, Xi'an Shaanxi, P. R. China 710032. panyong20@hotmail.com
Ai
Yu-feng
YF
Huang
Wei
W
D000818:Animals; D001705:Bioprosthesis; D001807:Blood Vessel Prosthesis; D003094:Collagen; D004730:Endothelium, Vascular / Q000166:cytology*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D011100:Polyglycolic Acid; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000379:methods*
D011100:Polyglycolic Acid; D003094:Collagen