NO induced novel DNA lesions: formation mechanism.
2'-Deoxyguanosine was treated with NO/O2 mixture at pH 7.0-7.8, and as well as the known major products such as 2'-deoxyxanthosine and 2'-deoxyoxanosine, some unidentified products were detected by RP-HPLC. In the present study, one of them has been characterized as a novel lesion, N2-nitro-2'-deoxyguanosine by spectrometric and chromatographic analysis. The mechanism for the production is also discussed.
Yamada
M
M
Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.
Suzuki
T
T
Kanaori
K
K
Tajima
K
K
Morii
T
T
Makino
K
K
D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000737:chemistry* / Q000187:drug effects* / Q000378:metabolism; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000737:chemistry*; D066298:In Vitro Techniques; D008956:Models, Chemical; D015394:Molecular Structure; D009569:Nitric Oxide / Q000633:toxicity*; D010100:Oxygen
D009569:Nitric Oxide; D004247:DNA; D003849:Deoxyguanosine; D010100:Oxygen