Frequent loss of heterozygosity on chromosome 10p15, a putative telomerase repressor/senescence gene locus, in gastric cancer.
Previous studies suggest that a telomerase repressor/senescence gene, which acts as a tumor suppressor gene, may be located on chromosome 10p15. However, there are no studies on alterations on chromosome 10p15 in gastric cancers. We, therefore, examined loss of heterozygosity (LOH) on the 10p15 in gastric cancers by microsatellite assay. Two microsatellite loci, D10S501 and D10S602, were used. Fifty-seven gastric cancers, including 36 intestinal type and 21 diffuse type, were selected. LOH at D10S602 and D10S501 was detected in 6 of 18 (33%) and 5 of 27 (19%) gastric cancers, respectively. There was no significant correlation between LOH at these loci and clinicopathologic features, including patient age, sex, tumor location, histologic subtype, depth of invasion, and lymph node metastasis. These data suggest that a putative telomerase repressor/senescence gene may be located on chromosome 10p15, especially at the D10S602, in gastric carcinogenesis, and that the putative gene malfunction may be involved in the early stages of gastric carcinogenesis.
Hiyama
Toru
T
Health Service Center, Hiroshima University, Higashihiroshima 739-8521, Japan. tohiyama@hiroshima-u.ac.jp
Tanaka
Shinji
S
Shima
Hideyuki
H
Kose
Kazuhiro
K
Kitadai
Yasuhiko
Y
Ito
Masanori
M
Sumii
Masaharu
M
Yoshihara
Masaharu
M
Shimamoto
Fumio
F
Haruma
Ken
K
Chayama
Kazuaki
K
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016922:Cellular Senescence; D002879:Chromosomes, Human, Pair 10; D004247:DNA / Q000737:chemistry; D005260:Female; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000235:genetics*; D019098:Telomerase / Q000378:metabolism*
D004247:DNA; D019098:Telomerase