Antioxidation properties and mechanism of action of dihydromyricetin from Ampelopsis grossedentata.
OBJECTIVE
To assess the antioxidative properties and the mechanism of action of dihydromyricetin (DMY) from Ampelopsis grossedentata.


METHODS
The antioxidative properties of DMY were measured by scavenging 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) and inhibiting lipid peroxidation induced by FeSO4-edetic acid in linoleic acid. The mechanism of antioxidative properties of DMY was tested by measuring the chelating activities of DMY for Fe2+ with ultraviolet spectrum (UV) method.


RESULTS
The specific absorption of DPPH radical solution at 517 nm was reduced 73.3%-91.5% when DMY was added into the reaction solution in the concentration range from 0.01% to 0.04%. DMY was shown to greatly inhibit the increase of lipid peroxidation (LPO) values in linolei acid system catalyzed by FeSO4-edetic acid. The reaction rates (A532.min-1) of lipid peroxidation were 0.0021-0.0004 in the concentration range from 0.01% to 0.04% and the inhibition activities of DMY was found to be in a concentration-dependent manner. The mechanism of antioxidative properties of DMY was chelating Fe2+ in the Fe(2+)-dependent lipid peroxidation system.


CONCLUSIONS
DMY showed great antioxidative effect and would be a good natural antioxidant.
Zhang
You-sheng
YS
College of Food and Biological Engineering, South China University of Science and Technology, Guangzhou 510641, China. youshengzhang@263.net
Ning
Zheng-xiang
ZX
Yang
Shu-zhen
SZ
Wu
Hui
H
D032421:Ampelopsis / Q000737:chemistry*; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D002614:Chelating Agents / Q000494:pharmacology; D044948:Flavonols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects*; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry
D000975:Antioxidants; D002614:Chelating Agents; D044948:Flavonols; D016166:Free Radical Scavengers; C472036:dihydromyricetin