Wild-type Escherichia coli producing microcins B17, D93, J25, and L; cloning of genes for microcin L production and immunity.
For the first time, an Escherichia coli strain producing four microcins (Mcc), B17, D93, J25, and L, and showing immunity to Mcc V was isolated and characterized. Each of the gene clusters encoding the production of Mcc B17, D93, and L was cloned separately. The gene cluster for Mcc L was cloned within a 13.5-kb HindIII-SalI fragment, which includes the Mcc V immunity gene, cvi.
Sablé
S
S
Laboratoire de génie protéique et cellulaire, Université de La Rochelle, Pôle Sciences, 17042 La Rochelle CEDEX 01, France.
Duarte
M
M
Bravo
D
D
Lanneluc
I
I
Pons
A M
AM
Cottenceau
G
G
Moreno
F
F
D001430:Bacteriocins / Q000096:biosynthesis* / Q000235:genetics* / Q000276:immunology / Q000494:pharmacology; D003001:Cloning, Molecular; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D005798:Genes, Bacterial; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data
D001430:Bacteriocins; C020411:microcin