[Investigation of connective tissue growth factor's gene cloning].
OBJECTIVE
The human connective tissue growth factor(CTGF), as the responsive product of immediate early gene, was cloned for the investigation of cellular primary gene responded and healing repairing.


METHODS
RT-PCR was performed with the mRNA of HUVE cell stimulated, the three clones of CTGF, via mutational modified, were ligated to a complete gene that identified with sequencing.


RESULTS
The sequencing indicated that this CTGF gene contains complete coding region and the 3'-noncoding region which is difference with the reported sequence.


CONCLUSIONS
A new human CTGF gene was cloned.
Li
Q
Q
Department of Viral and Immunology, Institute of Medical Biology, CAMS, PUMC, Kunming 650118, China. glimbcam@km.ym.cn
Zhao
H
H
Wang
J
J
Fu
J
J
Zhao
S
S
Jiang
L
L
D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D055513:Connective Tissue Growth Factor; D017781:Genes, Immediate-Early / Q000235:genetics*; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics*; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014471:Umbilical Veins / Q000166:cytology
C526317:CCN2 protein, human; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D055513:Connective Tissue Growth Factor