title: Inhibitory effects of human and porcine alpha-amylase on CCK-8-stimulated lipase secretion of isolated rat pancreatic acini.
abstract: Previously we have demonstrated inhibitory effects of the plant lectin wheat germ agglutinin (WGA) on (125)I-CCK-8 binding to pancreatic AR42J cells as well as on CCK-8-stimulated Ca(2+) release and alpha-amylase secretion of rat pancreatic acini or acinar cells. Therefore, it is entirely conceivable that alpha-amylase having several lectin-like carbohydrate recognition domains can modulate the CCK-8 stimulated lipase secretion. Human alpha-amylase, purified from pancreatic juice by affinity chromatography to homogeneity, and commercial porcine pancreatic alpha-amylase inhibit CCK-8-stimulated lipase secretion of rat pancreatic acini in a concentration-dependent manner. Acarbose, a specific inhibitor of alpha-amylase, was without effect on CCK-8-induced cellular lipase secretion. The data presented here provide evidence for a regulatory function of alpha-amylase in CCK-8-stimulated pancreatic secretion.
authors.affiliation: Department of Pathology, Faculty of Medicine, University of Rostock, Germany. ludwig.jonas@med.uni-rostock.de
authors.forename: Ludwig
authors.initials: L
authors.lastname: Jonas
authors.forename: Ulrike
authors.initials: U
authors.lastname: Mikkat
authors.forename: Renate
authors.initials: R
authors.lastname: Lehmann
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Schareck
authors.forename: Hermann
authors.initials: H
authors.lastname: Walzel
authors.forename: Werner
authors.initials: W
authors.lastname: Schröder
authors.forename: Hilja
authors.initials: H
authors.lastname: Lopp
authors.forename: Tönu
authors.initials: T
authors.lastname: Püssa
authors.forename: Peeter
authors.initials: P
authors.lastname: Toomik
mesh_terms: D000372:Agglutination Tests; D000595:Amino Acid Sequence; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D010189:Pancreatic Juice / Q000201:enzymology / Q000378:metabolism; D020799:Pancrelipase / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D012844:Sincalide / Q000494:pharmacology*; D013552:Swine; D000516:alpha-Amylases / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D020799:Pancrelipase; D000516:alpha-Amylases; D012844:Sincalide