YodA from Escherichia coli is a metal-binding, lipocalin-like protein.
We have determined the crystal structure of YodA, an Escherichia coli protein of unknown function. YodA had been identified under conditions of cadmium stress, and we confirm that it binds metals such as cadmium and zinc. We have also found nickel bound in one of the crystal forms. YodA is composed of two domains: a main lipocalin/calycin-like domain and a helical domain. The principal metal-binding site lies on one side of the calycin domain, thus making YodA the first metal-binding lipocalin known. Our experiments suggest that YodA expression may be part of a more general stress response. From sequence analogy with the C-terminal domain of a metal-binding receptor of a member of bacterial ATP-binding cassette transporters, we propose a three-dimensional model for this receptor and suggest that YodA may have a receptor-type partner in E. coli.
David
Gabriel
G
Laboratoire pour l'Utilisation du Rayonnement Electromagnétique, CNRS, CEA, MdR, BP. 34, 91898 Orsay Cedex, France.
Blondeau
Karine
K
Schiltz
Marc
M
Penel
Simon
S
Lewit-Bentley
Anita
A
D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D002104:Cadmium / Q000378:metabolism; D002455:Cell Division; D018360:Crystallography, X-Ray; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000502:physiology*; D007477:Ions; D054834:Lipocalins; D008074:Lipoproteins / Q000737:chemistry* / Q000378:metabolism; D008670:Metals; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D013050:Spectrometry, Fluorescence; D015032:Zinc / Q000378:metabolism
D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; D007477:Ions; D054834:Lipocalins; D008074:Lipoproteins; D008670:Metals; C470844:ZinT protein, E coli; C095894:blc protein, E coli; D002104:Cadmium; D000255:Adenosine Triphosphate; D015032:Zinc