Hypochlorite scavenging activity of hydroxycinnamic acids evaluated by a rapid microplate method based on the measurement of chloramines.
Scavengers of hypochlorite (XOCl) could have beneficial effects in diseases in which this oxidant plays a pathogenic role. It has been reported that ferulic acid and chlorogenic acid, the quinic ester of caffeic acid, are good hypochlorite scavengers, but a systematic evaluation of the naturally occurring hydroxycinnamic acids (HCAs), which these substances belong to, has not been performed yet. Thus, in this work we studied, by two different in-vitro methods, the antioxidant activity of five HCAs: p-coumaric acid, ferulic acid, sinapinic acid, caffeic acid and chlorogenic acid. The methods applied in this study were based on the oxidation of human serum albumin (HSA) by XOCl, a new microplate method based on the measurement of chloramines and a previously described carbonyl assay. Firstly, lysine-derived chloramines, in the presence or absence of the HCAs, were detected using 5-thio-2-nitrobenzoic acid (TNB), measuring the absorbance at 415 nm by a microplate reader. To remove excess XOCl, Trolox, a known XOCl scavenger, was added before TNB. Secondly, lysine-derived carbonyls, in the presence or absence of the HCAs, were detected by using 2,4-dinitrophenylhydrazine. Hydroxycinnamic acids appeared active (caffeic >/= sinapinic > chlorogenic congruent with ferulic > p-coumaric acid) by both methods, suggesting possible pharmacological applications for these compounds, which are present at high concentrations in the plant kingdom.
Firuzi
Omidreza
O
Dipartimento di Farmacologia delle Sostanze Naturali e Fisiologia Generale, Università di Roma La Sapienza, P.le Aldo Moro 5, 00185 Rome, Italy.
Giansanti
Luisa
L
Vento
Roberta
R
Seibert
Cathrin
C
Petrucci
Rita
R
Marrosu
Giancarlo
G
Agostino
Roberta
R
Saso
Luciano
L
D002700:Chloramines / Q000737:chemistry*; D003373:Coumaric Acids / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry*; D006997:Hypochlorous Acid / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods
D002700:Chloramines; D003373:Coumaric Acids; D016166:Free Radical Scavengers; D012709:Serum Albumin; D006997:Hypochlorous Acid