title: In vitro selection of N peptide-binding RNA on a quartz-crystal microbalance.
abstract: We report here an in vitro selection of a hairpin loop-RNA bound to N Peptide from bacteriophage lambda on a 27 MHz quartz-crystal microbalance (QCM) to study an interaction between an RNA structure and an RNA-binding peptide. The N Peptide was immobilized on a QCM electrode and specific RNAs binding to the peptide were selected from a GNRNA pentaloop-randomized RNA library with in situ monitoring of selection processes using a QCM. After the 5th round selection, the consensus sequences including a GCGCA loop were obtained from the pentaloop library. This RNA sequence was different from a binding site of a native N Protein (boxB RNA).
authors.affiliation: Department of Biomolecular Engineering, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Murakawa
authors.forename: Shinobu
authors.initials: S
authors.lastname: Fukusho
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Furusawa
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Okahata
mesh_terms: D001483:Base Sequence; D009690:Nucleic Acid Conformation; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D011791:Quartz; D012313:RNA / Q000378:metabolism*
chemical_list: D010455:Peptides; D011791:Quartz; D012313:RNA