title: Sodium pentobarbital anesthesia transiently enhances the severity of infection following intragastric, but not intravenous, inoculation of Listeria monocytogenes in mice.
abstract: In the present study we observed that mice anesthetized with sodium pentobarbital were far more susceptible to gastrointestinal challenge with Listeria monocytogenes than were unanaesthetized mice. The effect of pentobarbital anesthesia was transient (gone within 2 h) and did not alter the severity of infection following i.v. challenge with L. monocytogenes. Treatment with pharmacological inhibitors of gastric acidity (i.e. cimetidine and omeprazole), or intestinal motility (loperamide), did not duplicate the effect of pentobarbital on gastrointestinal listeriosis. These findings suggest that sodium pentobarbital anesthesia causes a short-lived but striking diminution in resistance to gastrointestinal listeriosis in mice, via an undefined mechanism.
authors.affiliation: Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, 2015 Linden Drive, Madison, WI 53706, USA. czuprync@svm.vetmed.wisc.edu
authors.forename: Charles J
authors.initials: CJ
authors.lastname: Czuprynski
authors.forename: Nancy G
authors.initials: NG
authors.lastname: Faith
authors.forename: Howard
authors.initials: H
authors.lastname: Steinberg
authors.forename: Brien
authors.initials: B
authors.lastname: Neudeck
mesh_terms: D000758:Anesthesia; D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology; D000930:Antidiarrheals / Q000494:pharmacology; D018929:Cell Culture Techniques; D002460:Cell Line; D002927:Cimetidine / Q000494:pharmacology; D015169:Colony Count, Microbial; D005767:Gastrointestinal Diseases / Q000276:immunology / Q000382:microbiology / Q000503:physiopathology*; D006801:Humans; D006993:Hypnotics and Sedatives / Q000008:administration & dosage / Q000633:toxicity*; D007113:Immunity, Innate / Q000187:drug effects*; D008089:Listeria monocytogenes / Q000472:pathogenicity*; D008088:Listeriosis / Q000276:immunology / Q000382:microbiology / Q000503:physiopathology*; D008099:Liver / Q000382:microbiology; D008139:Loperamide / Q000494:pharmacology; D051379:Mice; D009853:Omeprazole / Q000494:pharmacology; D010424:Pentobarbital / Q000008:administration & dosage / Q000633:toxicity*; D013154:Spleen / Q000382:microbiology
chemical_list: D000897:Anti-Ulcer Agents; D000930:Antidiarrheals; D006993:Hypnotics and Sedatives; D008139:Loperamide; D002927:Cimetidine; D010424:Pentobarbital; D009853:Omeprazole