[Mutations in the D-loop region of mitochondrial DNA in ovarian tumors].
OBJECTIVE
To investigate mutations in the D-loop region of mitochondrial DNA in ovarian tumors.


METHODS
The D-loop region of 25 epithelial ovarian tumors together with the adjacent normal tissues were amplified by PCR and sequenced.


RESULTS
Among the 25 ovarian tumors, 26 mutations were identified with the mutation rate of 32%. 19 mutations were detected in two cases of borderline carcinoma which was a special type of epithelial ovarian carcinoma. There were 6 microsatellite instabilities among the mutations and 11 new polymorphisms which were not reported previously in the GenBank.


CONCLUSIONS
The D-loop region of mitochondrial DNA is a highly polymorphoric and mutable region and the mutation rate is relatively high in patients with ovarian tumors.
Shi
Hong-hui
HH
Department of Obstetrics and Gynecology, PUMC Hospital, CAMS, PUMC, Beijing 100730, China.
Vincent
Liu
L
Hextan
Ngan
N
Yang
Xiu-yu
XY
D018284:Cystadenocarcinoma, Serous / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D006801:Humans; D010051:Ovarian Neoplasms / Q000235:genetics*; D017354:Point Mutation; D011110:Polymorphism, Genetic
D004272:DNA, Mitochondrial