[Preparation of solid lipid nanoparticles by solvent diffusion method].
OBJECTIVE
To establish an efficient method for preparation of solid lipid nanoparticles with high recovery.


METHODS
Monostearin solid lipid nanoparticles was prepared by solvent diffusion method in aqueous system. The recovery of the method was greatly improved by adjusting the Zeta potential.


RESULTS
The drug-loaded solid lipid nanoparticles suspension was quickly produced and easily separated with centrifugation at 4,000 r.min-1 under acidic condition. Compared with the nanoparticles made without adjusting the Zeta potential, the recovery of nanoparticles prepared in this way was significantly increased. The release behavior in vitro showed an initial burst effect in the first 3 hours followed by a slower rate stage of 4 days with nearly 6% drug released in each day.


CONCLUSIONS
The solvent diffusion method in aqueous system might be used as a new method to prepare solid lipid nanoparticles in the future. The loaded drug can be released in a controlled manner.
Zhang
Hui-hong
HH
College of Pharmacy, Zhejiang University, Hangzhou 310006, China. xizilake365@sohu.com
Hu
Fu-qiang
FQ
Yuan
Hong
H
Fang
Ming
M
D002990:Clobetasol / Q000008:administration & dosage / Q000031:analogs & derivatives*; D003692:Delayed-Action Preparations / Q000737:chemistry; D004058:Diffusion; D004337:Drug Carriers; D016503:Drug Delivery Systems; D005989:Glycerides / Q000737:chemistry*; D036103:Nanotechnology; D010316:Particle Size; D012997:Solvents; D013678:Technology, Pharmaceutical / Q000379:methods*
D003692:Delayed-Action Preparations; D004337:Drug Carriers; D005989:Glycerides; D012997:Solvents; C048159:glyceryl monostearate; D002990:Clobetasol