[Immunofluorescence identification of limbal stem cells in a culture of donor corneas].
The purpose of this study was to identify limbal stem cells in the culture of human donor cornea. After standard cultivation procedure we used monoclonal antibody against alpha-enolase for identification of stem cells. This antibody serves as a probe for the identification of biochemical marker of stem cells. Using immunofluorescent microscopy we identified in specimens alpha-enolase positive cells which in vitro but not in situ showed also some morphologic differences from other cellular elements of the culture.
Kozák
I
I
Ocná klinika LF UPJS, Kosice.
Rosocha
J
J
Hrbková
H
H
Juhás
T
T
D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D016039:Corneal Transplantation; D005455:Fluorescent Antibody Technique; D006801:Humans; D016850:Limbus Corneae / Q000166:cytology*; D010751:Phosphopyruvate Hydratase / Q000032:analysis* / Q000276:immunology; D013234:Stem Cells / Q000737:chemistry / Q000166:cytology*
D000911:Antibodies, Monoclonal; D010751:Phosphopyruvate Hydratase