[The transcriptional regulation study on human delta globin gene with CAAT box C-->T point mutation in its promoter].
OBJECTIVE
To study the transcriptional regulation of human delta globin gene with C-->T point mutation at -64 in its promoter.


METHODS
Human delta globin genes including wild CAAT box and mutant CAAT box (-64C-->T) were separately cloned into eukaryotic expression vector pcDNA3.1 (-)/Myc-His A which was cut out the strong promoter CMV, transfected MEL cells, and induced by DMSO to express. The transcriptional regulation of human delta globin gene was analysed using semi-quantitative RT-PCR.


RESULTS
The expression level of human delta globin gene with mutant CAAT box was 2.2-fold as high as that with wild CAAT box.


CONCLUSIONS
The defective CAAT box of human delta globin gene promoter region may be one of the major reasons for its low expression level.
Yao
Jie
J
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China.
Chen
Song-sen
SS
Yang
Ke-gong
KG
Di
Xu
X
Xiong
An-qi
AQ
Zhang
You-hong
YH
D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics*; D005914:Globins / Q000235:genetics*; D006801:Humans; D017354:Point Mutation; D011401:Promoter Regions, Genetic / Q000235:genetics; D014158:Transcription, Genetic
D022762:CCAAT-Enhancer-Binding Proteins; D005914:Globins