title: Cuticular Architecture of Hassalstrongylus epsilon (Nematoda: Trichostrongyloidea).
abstract: Hassalstrongylus epsilon is a small nematode, whose adult forms are found among the intestinal microvilli of the water rat Nectomys squamipes, Brants 1827 (Rodentia: Muridae). The external appearance of the cuticle, which presents transversal striations and longitudinal ridges, is described using scanning electron microscopy. Transmission electron microscopy of thin sections and replicas of quick-frozen, freeze-fractured, deep-etched and rotatory shadowed samples showed the presence in the cuticle of struts that arise from the fluid median layer, extending outward to the epicuticle. The cuticle showed the presence of five layers: epicuticle, cortical, fibril-rich, fluid median and fibrous. The cuticle layers were made of an assemblage of fibers that create compartments, which were larger in the fluid region than in the fibril-rich median layer.
authors.affiliation: Laboratório de Helmintologia, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Bloco G, 21949-900 Ilha do Fundão, Rio de Janeiro, RJ, Brazil.
authors.forename: A
authors.initials: A
authors.lastname: Oliveira-Menezes
authors.forename: W
authors.initials: W
authors.lastname: De Souza
authors.forename: R M
authors.initials: RM
authors.lastname: Lanfredi
mesh_terms: D000818:Animals; D005613:Freeze Etching / Q000379:methods; D005614:Freeze Fracturing / Q000379:methods; D007421:Intestine, Small / Q000166:cytology / Q000469:parasitology*; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D008871:Microvilli / Q000469:parasitology / Q000648:ultrastructure; D009115:Muridae / Q000469:parasitology*; D051381:Rats; D016626:Shadowing Technique, Histology / Q000379:methods; D014251:Trichostrongyloidea / Q000648:ultrastructure*