Role of cytokeratins, nuclear matrix proteins, Lewis antigen and epidermal growth factor receptor in human bladder tumors.
The content of urinary bladder cancer antigen (UBC), tissue polypeptide specific antigen (TPS), nuclear matrix protein 22 (NMP22), and the expression of LewisY carbohydrate antigens and of epidermal growth factor receptor (EGF-R) in bladder tumor tissues were determined. These included 14 well, 6 moderately and 11 poorly differentiated bladder cancers. Cytosol UBC and TPS were higher in the well and in the moderately differentiated bladder tumors than in the poorly differentiated bladder cancers; whereas cytosol NMP22 was higher in the poorly differentiated bladder cancers than in the well and in the moderately differentiated bladder tumors. The Lewis related carbohydrate antigens, evaluated by the reactivity of the tissues to monoclonal antibody B3, were highly expressed in poorly differentiated tumors. The EGF-R was strongly expressed in a large number of poorly differentiated bladder tumors. These data suggest that the determination of cytosol NMP22 and the immunoblotting with B3 and EGF-R antibodies might be useful to obtain more information on the differentiation of bladder tumors.
Di Carlo
Angelina
A
Dipartimento di Biologia e Patologia Cellulare e Molecolare L. Califano, Università degli Studi di Napoli Federico II, Via S. Pansini 5, I-80131 Naples, Italy.
Terracciano
Daniela
D
Mariano
Angela
A
Oliva
Andrea
A
D'Armiento
Massimo
M
Macchia
Vincenzo
V
D000368:Aged; D000369:Aged, 80 and over; D002241:Carbohydrates / Q000737:chemistry; D002454:Cell Differentiation; D002462:Cell Membrane / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D066246:ErbB Receptors / Q000502:physiology*; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007633:Keratins / Q000502:physiology*; D007983:Lewis Blood Group Antigens / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D034521:Nuclear Matrix-Associated Proteins / Q000502:physiology*; D009687:Nuclear Proteins / Q000096:biosynthesis; D001749:Urinary Bladder Neoplasms / Q000378:metabolism* / Q000473:pathology
D002241:Carbohydrates; D007983:Lewis Blood Group Antigens; D034521:Nuclear Matrix-Associated Proteins; D009687:Nuclear Proteins; C100379:nuclear matrix protein 22; D007633:Keratins; D066246:ErbB Receptors