title: Kinetic analysis of nucleotides incorporated opposite oxidized thymine bases on template DNA.
abstract: Incorporation of nucleotides opposite the oxidized thymine bases on the template by eukaryotic DNA polymerases alpha and gamma was studied using the synthetic oligonucleotides containing 5-formyluracil or 5-hydroxymethyluracil. Primer extension assay revealed that both polymerases could bypass the oxidized thymine lesion, and incorporated dATP and dGTP opposite 5-formyluracil on the template. On the other hand, dATP was an only nucleotide that was incorporated opposite 5-hydroxymethyluracil by both polymerases as far as examined. These results indicate that 5-formyluracil but not 5-hydroxymethyluracil on template DNA may induce the transition mutation of A:T to G:C in eukaryotes.
authors.affiliation: Graduate School of Science and Technology, Kumamoto University, Kumamoto 860-8555, Japan.
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Miyashita
authors.forename: Akira
authors.initials: A
authors.lastname: Ono
authors.forename: Shunji
authors.initials: S
authors.lastname: Izuta
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000378:metabolism*; D017931:DNA Primers; D007700:Kinetics; D010084:Oxidation-Reduction; D013698:Templates, Genetic; D013941:Thymine / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D004247:DNA; D013941:Thymine