Direct spectrometric determination of proteins in body fluids using a near-infrared cyanine dye.
A new near-infrared (NIR) dye, 1,1'-disulfobutyl-3,3,3',3'-tetramethylindotricarbocyanine (DTCY) has been developed for the quantitation of proteins in solution. The method is based on the binding of DTCY to proteins under acidic conditions. The binding of DTCY to proteins causes a new band at 814 nm. The maximum binding number of bovine serum albumin (BSA) with DTCY was measured as 100. The linear range is 0.3-40 microg mL(-1) for BSA and human serum albumin (HSA), respectively. Except for Fe(2+), Cu(2+), and cetyltrimethylammonium bromide, all of the examined coexisting substances show no interference in the assay. The method has been applied to the quantitation of proteins in serum and urine with recoveries between 96 and 105%.
Li
Wei-Rong
WR
Department of Chemistry, Wuhan University, 430072 Wuhan, P.R. China.
Wang
Hong
H
Yang
Ting-Xian
TX
Zhang
Hua-Shan
HS
D001826:Body Fluids / Q000737:chemistry*; D002138:Calibration; D002232:Carbocyanines / Q000737:chemistry*; D011489:Protein Denaturation; D011506:Proteins / Q000032:analysis*; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
C479728:1,1'-disulfobutyl-3,3,3',3'-tetramethylindotricarbocyanine; D002232:Carbocyanines; D011506:Proteins; D012710:Serum Albumin, Bovine