title: Leukocyte motility in response to neuropeptides is heparan sulfate proteoglycan dependent.
abstract: Activation of neuropeptide receptors on leukocytes induces chemotaxis. We determined in Boyden chambers with micropore filters, whether in human monocytes and lymphocytes this migratory response is heparan sulfate proteoglycan (HSPG) dependent. Chemotaxis toward calcitonin gene-related peptide, secretoneurin, vasoactive intestinal peptide (VIP), and substance P (SP) was abolished by removal of heparan sulfate side chains from cell surface proteoglycans or by addition of anti-syndecan-4 antibodies. Inhibition of neuropeptide-induced chemotaxis by dimethyl sphingosine (DMS), an inhibitor of sphingosine kinase, indicates transactivation of the sphingosine-1-phosphate chemotaxis pathway which was previously identified as being syndecan-4-related. Data suggest that HSPGs are involved in neuropeptide-induced chemotaxis of leukocytes.
authors.affiliation: Division of General Internal Medicine, Department of Internal Medicine, University of Innsbruck, Anichstrasse 35, A-6020 Innsbruck, Austria.
authors.forename: Nicole C
authors.initials: NC
authors.lastname: Kaneider
authors.forename: Petra
authors.initials: P
authors.lastname: Egger
authors.forename: Angela M
authors.initials: AM
authors.lastname: Djanani
authors.forename: Christian J
authors.initials: CJ
authors.lastname: Wiedermann
mesh_terms: D000906:Antibodies / Q000494:pharmacology; D002633:Chemotaxis / Q000187:drug effects*; D002810:Chondroitinases and Chondroitin Lyases / Q000502:physiology; D019812:Heparan Sulfate Proteoglycans / Q000037:antagonists & inhibitors / Q000502:physiology*; D019763:Heparin Lyase / Q000502:physiology; D006801:Humans; D007962:Leukocytes / Q000187:drug effects / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000378:metabolism; D009479:Neuropeptides / Q000378:metabolism / Q000494:pharmacology*; D011509:Proteoglycans / Q000378:metabolism; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000502:physiology; D053671:Syndecan-4
chemical_list: D000906:Antibodies; D019812:Heparan Sulfate Proteoglycans; D008562:Membrane Glycoproteins; D009479:Neuropeptides; D011509:Proteoglycans; C508304:SDC4 protein, human; D053671:Syndecan-4; D002810:Chondroitinases and Chondroitin Lyases; D019763:Heparin Lyase; C061800:N,N-dimethylsphingosine; D013110:Sphingosine