Intramolecular dimers: a new design strategy for fluorescence-quenched probes.
Fluorogenic probes dual-labeled with reporter and quencher dyes use a change in fluorescence to monitor biochemical events (e.g., substrate binding or enzyme digestion). Such events change the reporter-quencher distance, which affects fluorescence. Recently, it is has been shown that static quenching through intramolecular dimers is an important mechanism that can sometimes be more efficient than Förster resonance energy transfer (FRET).
Johansson
Mary Katherine
MK
Biosearch Technologies, 81 Digital Drive, Novato, CA 94949, USA. info@biosearchtech.com
Cook
Ronald M
RM
D002232:Carbocyanines / Q000737:chemistry; D019281:Dimerization; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D015345:Oligonucleotide Probes / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D012235:Rhodamines / Q000737:chemistry
D002232:Carbocyanines; D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D010455:Peptides; D012235:Rhodamines; C085321:cyanine dye 5