Argemone oil induced cellular damage in the reproductive tissues of transgenic Drosophila melanogaster: protective role of 70 kDa heat shock protein.
We explored the reproductive toxicity of argemone oil and its principal alkaloid fraction in transgenic Drosophila melanogaster (hsp70-lacZ) Bg(9). The toxicity of argemone oil has been attributed to two of its physiologically active benzophenanthridine alkaloids, sanguinarine and dihydrosanguinarine. Freshly eclosed first instar larvae of transgenic Drosophila melanogaster were transferred to different concentrations of argemone oil and its alkaloid fraction contaminated food. Virgin flies that eclosed from the contaminated food were pair-mated to look into the effect on reproduction. The study was further extended by investigating hsp70 expression and tissue damage in larval gonads, genital discs, and reproductive organs of adult fly. Our results showed that argemone oil was more cytotoxic than its principal alkaloid fraction. Moreover, it was the male fly that was more affected compared to its opposite number. The accessory glands of male reproductive system of the fly, which did not express hsp70, exhibited severe damage as evidenced by Trypan blue staining. This prompted us to explore the ultrastructural morphology of the gland, which showed acute signs of necrosis in both the cell types as evident by necrotic nuclei, higher vacuolization, and disorganized endoplasmic reticulum, decrease in the number of Golgi vesicles and disorganized, loosely packed filamentous structures in the lumen of the accessory gland, at the higher concentrations of the adulterant. The study showed the reproductive toxicity of argemone oil and its alkaloid fraction in transgenic Drosophila melanogaster and further confirmed the cytoprotective role of hsp70.
Mukhopadhyay
Indranil
I
Embryotoxicology Section, Industrial Toxicology Research Centre, Lucknow 226001, Uttar Pradesh, India.
Saxena
D K
DK
Bajpai
V K
VK
Chowdhuri
D Kar
DK
D000470:Alkaloids / Q000633:toxicity; D000818:Animals; D030801:Animals, Genetically Modified; D004331:Drosophila melanogaster / Q000166:cytology / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D005260:Female; D005835:Genitalia / Q000187:drug effects* / Q000473:pathology* / Q000648:ultrastructure; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D010938:Plant Oils / Q000633:toxicity*; D015203:Reproducibility of Results; D012098:Reproduction / Q000187:drug effects / Q000502:physiology; D013997:Time Factors; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D000470:Alkaloids; D018840:HSP70 Heat-Shock Proteins; D010938:Plant Oils; C030610:argemone oil; D001616:beta-Galactosidase