Preparation of anti-idiotypic antibodies specific for anti-HEL and analysis of their functional mimicry.
OBJECTIVE
This study is to investigate the functional mimicry by using anti-idiotypic antibodies of enzymes.


METHODS
Monoclonal anti-idiotypic antibodies against anti-HEL (hen egg-white lysozyme, HEL) antibodies were obtained by fusion of Sp2/0 myeloma cells with spleen cells of syngeneic mice immunized with monoclonal anti-HEL antibodies against HEL's different antigenic epitopes. Then bacteriolysis of the anti-idiotypic antibodies were observed.


RESULTS
Eight hybridomas strains secreting anti-idiotypic antibodies were selected and characterized. It was shown that two of eight anti-idiotypic antibodies secreted by two hybridomas (1A10C9 and 2A11C1B3) could mimic HEL catalytic activity to lyse Micrococcus lysodeikticus and that the catalytic effect of mixed anti-idiotypic antibodies of 1A10C9 and 2A11C1B3 was stronger than that of one of them, but less than HEL.


CONCLUSIONS
The results demonstrated that the anti-idiotypic antibodies that could mimic enzyme activity existed in the idiotype network during anti-enzymatic immune response.
Mingyuan
L
L
Department of Microbiology, West China University of Medical Sciences, Chengdu 610041.
Yu
X
X
Liying
X
X
Hong
L
L
Zhonghua
J
J
Jiawan
M
M
Daoruo
W
W
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D002645:Chickens; D004529:Egg White / Q000032:analysis; D000939:Epitopes; D005260:Female; D006825:Hybridomas / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008837:Micrococcus / Q000276:immunology; D009113:Muramidase / Q000276:immunology* / Q000302:isolation & purification
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000939:Epitopes; D009113:Muramidase