title: [The expression of A103 and inhibin alpha in adrenocortical adenoma by high-throughput tissue microarray techniques].
abstract: OBJECTIVE
  To evaluate the potential diagnostic role of A103 and inhibin alpha in adrenocortical adenoma and the feasibility of tissue microarray/tissue chips.
  
  
  METHODS
  A series of adrenal gland tissue chips were prepared, including 79 cases of formalin-fixed, paraffin-embedded tissue from 3 normal adrenal glands, 66 adrenocortical adenomas, 5 cases of metastatic carcinoma and 5 pheochromocytomas. A103 and inhibin alpha were detected with Envision immunohistochemical staining methods.
  
  
  RESULTS
  Positive staining was seen in 3 of 3 (100%) adrenal cortices and in 55 of 61(90.2%) adrenocortical adenomas but in none of the pheochromocytomas or adrenal metastatic carcinomas. In the 3 of 3(100%) adrenal cortices, inhibin alpha immunoreactivity was limited to the zona reticularis and the innermost zona fasciculata. 51 of 61(83.6%) adrenocortical adenomas reacted with inhibin alpha. None of the pheochromocytomas or metastatic carcinomas reacted with inhibin alpha.
  
  
  CONCLUSIONS
  High-throughput tissue microarray/tissue chip offers a powerful strategy for rapid identification in situ. It is feasible to utilize tissue microarray for a rapid, economic and accurate screening of tissue specimens on a large scale. The combined use of A103 and inhibin alpha is valuable in distinguishing adrenocortical adenoma from pheochromocytoma and other metastatic carcinomas.
authors.affiliation: Department of Pathology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Hongying
authors.initials: H
authors.lastname: Zhang
authors.forename: Weiping
authors.initials: W
authors.lastname: Liu
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Wang
authors.forename: Gandi
authors.initials: G
authors.lastname: Li
authors.forename: Jia
authors.initials: J
authors.lastname: Guo
authors.forename: Fengyuan
authors.initials: F
authors.lastname: Li
authors.forename: Dianying
authors.initials: D
authors.lastname: Liao
mesh_terms: D000302:Adrenal Cortex / Q000378:metabolism; D000306:Adrenal Cortex Neoplasms / Q000378:metabolism*; D018246:Adrenocortical Adenoma / Q000378:metabolism*; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D006801:Humans; D007265:Inhibins / Q000096:biosynthesis* / Q000235:genetics; D058965:MART-1 Antigen; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D058965:MART-1 Antigen; C548667:MLANA protein, human; D009363:Neoplasm Proteins; C094377:inhibin-alpha subunit; D007265:Inhibins