[Determination of deoxyribonucleic acid with pararosaniline by a resonance light-scattering method].
The method is based on the enhancement effect of the resonance light-scattering (RLS) of pararosaniline by DNA. In the pH rang of 0.5-1.5, the enhanced intensity of pararosaniline RLS at 355 nm is proportional to the concentration of DNA in the rang of 0.20-15 micrograms.mL-1. The detection limit is 36 ng.mL-1. This method is simple, rapid and has been applied satisfactorily to the determination of DNA in mixed samples.
Xiang
Hai-yan
HY
Printed Circuit Board Factory, Zhuzhou Electric Locomotive Institute, Zhuzhou 412001, China.
Chen
Xiao-ming
XM
Zhou
Di-wu
DW
D000818:Animals; D004247:DNA / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D012394:Rosaniline Dyes; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry / Q000379:methods; D014052:Toluidines
D012394:Rosaniline Dyes; D014052:Toluidines; C005409:pararosaniline; D004247:DNA