The pH-induced glycosylation of secreted phosphatases is mediated in Aspergillus nidulans by the regulatory gene pacC-dependent pathway.
In this communication, we show that the pacC(c)14 mutation drastically reduced the mannose and N-acetylglycosamine content of the pacA-encoded acid phosphatase secreted by the fungus Aspergillus nidulans when grown at 22 degrees C, pH 5.0, compared to a control strain. The staining after PAGE was not observed for the pacA-encoded acid phosphatase, while the palD-encoded Pi-repressible alkaline phosphatase had an altered electrophoretic mobility. In addition, the secreted acid phosphatase also had a reduced number of isoforms visualized by staining after IEF and glycosylation had a protective effect against its heat inactivation. We also show that a full-length version of gene pacC-1 cloned from Neurospora crassa complemented the pacC(c)14 mutation of A. nidulans, including the remediation of both the acid and alkaline Pi-repressible phosphatases secreted at pH 5.0, which indicates that glycosylation of secreted phosphatases is mediated in A. nidulans by the conserved PacC pathway that governs pH-responsive gene expression.
Nozawa
S R
SR
Departamento de Genética, FMRP-USP, Ribeirão Preto, SP 14049-900, Brazil.
Ferreira-Nozawa
M S
MS
Martinez-Rossi
N M
NM
Rossi
A
A
D000135:Acid Phosphatase / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000096:biosynthesis / Q000378:metabolism; D001233:Aspergillus nidulans / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D002845:Chromatography; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins; D005800:Genes, Fungal; D005809:Genes, Regulator; D005816:Genetic Complementation Test; D006031:Glycosylation; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D009005:Monosaccharides / Q000032:analysis; D009154:Mutation; D009492:Neurospora crassa / Q000235:genetics; D010744:Phosphoric Monoester Hydrolases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D013194:Staining and Labeling; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*
D004271:DNA, Fungal; D005656:Fungal Proteins; D009005:Monosaccharides; C092394:PacC protein, Aspergillus; D014157:Transcription Factors; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D010744:Phosphoric Monoester Hydrolases