title: Morphological modifications in clitoris and vagina in spontaneously hypertensive rats.
abstract: We evaluated possible morphological alteration in clitoris and vagina from spontaneous hypertensive rats (SHR) and normotensive WKY rats. Clitoris and vagina were processed by Masson's trichrome, anti-alpha-smooth-muscle actin, anticollagen type I (COL I) and type III (COL III), and anti-TGFbeta(1). SHR presented higher amount of clitoral cavernous smooth muscle (CSM), vascular smooth muscle; TGFbeta(1) in clitoral vessel wall; higher wall/lumen ratio in both vaginal and clitoral vessels; and remarkable interstitial fibrosis, expressed by a higher amount in interstitial COL I and III in both clitoris and vagina, compared to WKY rats. Nerve fibers from clitoral and vaginal tissue in SHR showed important fibrosis at perineurium. SHR showed positive correlation between systolic blood pressure (SBP) and clitoral CSM; SBP and fibrosis in clitoris; and SBP and COL I and III in clitoris, respectively. Similar findings were observed between SBP and COL I and III in vagina. In conclusion, SHR present morphologic changes in clitoral vessels as well as in clitoral cavernous space, which have a high positive correlation with the high blood pressure level. Moreover, the increase in extracellular matrix affects not only the clitoral and vaginal interstitium but also the nerve structures from both clitoris and vagina.
authors.affiliation: Sexual Dysfunction Section, Urology Division, Hospital Durand, Buenos Aires, Argentina.
authors.forename: A J
authors.initials: AJ
authors.lastname: Bechara
authors.forename: G
authors.initials: G
authors.lastname: Cao
authors.forename: A R
authors.initials: AR
authors.lastname: Casabé
authors.forename: S V
authors.initials: SV
authors.lastname: Romano
authors.forename: J E
authors.initials: JE
authors.lastname: Toblli
mesh_terms: D000818:Animals; D001794:Blood Pressure; D001808:Blood Vessels / Q000473:pathology; D002987:Clitoris / Q000098:blood supply / Q000294:innervation / Q000378:metabolism / Q000473:pathology*; D024042:Collagen Type I / Q000378:metabolism; D024061:Collagen Type III / Q000378:metabolism; D005260:Female; D005355:Fibrosis; D006973:Hypertension / Q000473:pathology* / Q000503:physiopathology; D009130:Muscle, Smooth / Q000473:pathology; D009131:Muscle, Smooth, Vascular / Q000473:pathology; D009412:Nerve Fibers / Q000473:pathology; D010525:Peripheral Nerves; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D014621:Vagina / Q000098:blood supply / Q000294:innervation / Q000378:metabolism / Q000473:pathology*
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III