Modeling stability of photoheterotrophic continuous cultures in photobioreactors.
Continuous cultures of the purple non-sulfur bacterium Rhodospirillum rubrum were grown in a cylindrical photobioreactor in photoheterotrophic conditions, using acetate as carbon source. A new kinetic and stoichiometric knowledge model was developed, and its ability to simulate experimental results obtained under varying incident light fluxes and residence times is discussed. The model accurately predicts the stable, unstable, or oscillating behavior observed for the reactor productivity. In particular, the values of residence time corresponding to a subcritical bifurcation with a typical hysteresis effect are calculated and analyzed. The robustness of the proposed model allows the engineering operating domain of the photobioreactor function to be set and offers a promising tool for the design and control of such photoheterotrophic processes.
Cornet
Jean-François
JF
Laboratoire de Génie Chimique et Biochimique, Université Blaise Pascal-CUST 24, avenue des Landais, BP 206, F 63174 AUBIERE Cedex, France.
Favier
Lidia
L
Dussap
Claude-Gilles
CG
D000085:Acetates / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division / Q000502:physiology / Q000528:radiation effects; D003198:Computer Simulation; D004307:Dose-Response Relationship, Radiation; D006706:Homeostasis / Q000502:physiology*; D008027:Light; D008954:Models, Biological; D017711:Nonlinear Dynamics; D018462:Photobiology / Q000379:methods*; D015203:Reproducibility of Results; D012244:Rhodospirillaceae / Q000166:cytology / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects*; D012680:Sensitivity and Specificity
D000085:Acetates