title: Long-term effect of full-mouth tooth extraction on the responsiveness of peripheral blood monocytes.
abstract: BACKGROUND
  As some residual inflammation may remain after periodontal therapy, the present pilot study investigated the long-term effect of full-mouth tooth extraction therapy on the responsiveness of peripheral blood monocytes in a case with generalized terminal adult periodontitis.
  
  
  METHODS
  Before and 3, 9, 20 and 32 months after therapy, venous blood was collected. Total and differential white blood cell counts were determined and whole blood cell cultures (WBCC) were incubated with lipopolysaccharide (LPS) to stimulate the production of inflammatory mediators by monocytes.
  
  
  RESULTS
  After full-mouth tooth extraction, the numbers of total peripheral white blood cells and neutrophils decreased over time. The release of the chemokines interleukin (IL)-8 and macrophage chemoattractant protein (MCP)-1 in the cultures decreased twofold over time, whereas no changes were seen for the other studied cytokines, chemokines and prostaglandin E2.
  
  
  CONCLUSIONS
  On the basis of previous studies and the present case, the high production of IL-8 and MCP-1 by monocytes in LPS-stimulated WBCC from periodontitis patients is most likely acquired, as their levels decrease over time when the periodontal infection is controlled. The possible connection between periodontitis and atherosclerosis through IL-8 and MCP-1 is discussed.
authors.affiliation: Department of Periodontology, Academic Centre for Dentistry Amsterdam (ACTA), The Netherlands.
authors.forename: Schelte J
authors.initials: SJ
authors.lastname: Fokkema
authors.forename: Bruno G
authors.initials: BG
authors.lastname: Loos
authors.forename: Augustinus A M
authors.initials: AA
authors.lastname: Hart
authors.forename: Ubele
authors.initials: U
authors.lastname: van der Velden
mesh_terms: D000328:Adult; D001161:Arteriosclerosis / Q000097:blood; D018932:Chemokine CCL2 / Q000097:blood; D006801:Humans; D016209:Interleukin-8 / Q000097:blood; D007958:Leukocyte Count; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D009000:Monocytes / Q000737:chemistry; D010518:Periodontitis / Q000097:blood* / Q000601:surgery; D014081:Tooth Extraction
chemical_list: D018932:Chemokine CCL2; D016209:Interleukin-8; D008070:Lipopolysaccharides