Effect of EDTA on Salmonella enterica serovar Typhimurium involves a component not assignable to lipopolysaccharide release.
The effect of EDTA on Salmonella enterica serovar Typhimurium was studied in different growth phases with cells grown with or without Ca(2+) and Mg(2+) supplementation. EDTA affected the outer membrane much more strongly in the early exponential phase than in the mid- or late exponential phase, as indicated by uptake of 1-N-phenylnaphthylamine (a nonpolar hydrophobic probe, M(r) 219), and detergent (SDS) susceptibility. This effect was, however, not paralleled by LPS release (determined by measuring LPS-specific fatty acids or 14C-labelled LPS in cell-free supernatants, per a standardized cell density), which remained unchanged as a function of the growth curve. The conclusion from these results is that in the early exponential phase the effect of EDTA in S. enterica involves a component that is independent of LPS release.
Alakomi
H-L
HL
VTT Biotechnology, PO Box 1500, FIN-02044 VTT, Espoo, Finland.
Saarela
M
M
VTT Biotechnology, PO Box 1500, FIN-02044 VTT, Espoo, Finland.
Helander
I M
IM
Department of Applied Chemistry and Microbiology, Division of Microbiology, University of Helsinki, PO Box 56, FIN-00014 University of Helsinki, Finland.|VTT Biotechnology, PO Box 1500, FIN-02044 VTT, Espoo, Finland.
D015057:1-Naphthylamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000187:drug effects; D002614:Chelating Agents / Q000494:pharmacology*; D003902:Detergents / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000378:metabolism*; D008563:Membrane Lipids / Q000737:chemistry / Q000378:metabolism; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D012967:Sodium Dodecyl Sulfate / Q000378:metabolism
D002614:Chelating Agents; D003902:Detergents; D008070:Lipopolysaccharides; D008563:Membrane Lipids; D012967:Sodium Dodecyl Sulfate; C005444:N-phenyl-1-naphthylamine; D015057:1-Naphthylamine; D004492:Edetic Acid