Bioconversion of linoleic acid into conjugated linoleic acid during fermentation and by washed cells of Lactobacillus reuteri.
Conjugated linoleic acid (CLA) was produced at 300 mg l(-1) after 24 h culture of Lactobacillus reuteri in de Man-Rogosa-Sharpe medium containing 0.9 g linoleic acid (LA) l(-1) and 1.67% (v/v) Tween 80. CLA was mainly located in the extracellular space of the cells. Washed cells previously grown on LA were less active than unadapted washed cells in converting LA into CLA. Most of the CLA transformed by washed L. reuteri cells was located in cells or associated with cells. CLA production by washed L. reuteri cells was most efficient in conversion with 0.45 g LA l(-1) at pH 9.5 and 37 degrees C for 1 h.
Lee
Sun Ok
SO
Department of Bioscience and Biotechnology, Sejong University, Seoul 143-747, Korea.
Kim
Chang Sup
CS
Cho
Somi Kim
SK
Choi
Ha Jong
HJ
Ji
Geun Eog
GE
Oh
Deok-Kun
DK
D000222:Adaptation, Physiological / Q000502:physiology; D001711:Biotransformation; D007778:Lactobacillus / Q000254:growth & development* / Q000378:metabolism* / Q000502:physiology; D019787:Linoleic Acid / Q000737:chemistry* / Q000145:classification / Q000378:metabolism*; D012680:Sensitivity and Specificity; D013237:Stereoisomerism
D019787:Linoleic Acid