title: Overexpression of the Wilms' tumor gene WT1 in colorectal adenocarcinoma.
abstract: Expression of the Wilms' tumor gene WT1 was examined in 59 cases of colorectal adenocarcinoma to examine the involvement of WT1 in tumorigenesis. Quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) showed that WT1 mRNA was expressed in the range from 7.2 x 10(-5) to 4.9 x 10(-1) levels (WT1 expression level in K562 leukemic cells was defined as 1.0) in all (100%) of the 28 cases of colorectal adenocarcinoma examined, and that the WT1 mRNA expression levels were higher in 20 (71%) of the 28 cases compared to those of normal-appearing mucosal tissues examined. Immunohistochemical analysis using an anti-WT1 antibody was performed on 46 cases of colorectal adenocarcinoma (15 of the 28 cases with WT1 mRNA expression and 31 newly collected cases), and the expression of WT1 protein was detected in 41 (89%) of the 46 cases. The direct sequencing analysis of the WT1 genomic DNA showed no mutations in any of the 10 exons of the WT1 gene in any of 5 different colorectal adenocarcinomas. These results may indicate an important role of the wild-type WT1 gene in tumorigenesis of colorectal adenocarcinoma.
authors.affiliation: Department of Functional Diagnostic Science, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Oji
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Masaya
authors.initials: M
authors.lastname: Nomura
authors.forename: Yoko
authors.initials: Y
authors.lastname: Nakano
authors.forename: Ai
authors.initials: A
authors.lastname: Ikeba
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Nakatsuka
authors.forename: Sakie
authors.initials: S
authors.lastname: Abeno
authors.forename: Eiji
authors.initials: E
authors.lastname: Kiyotoh
authors.forename: Tanyarat
authors.initials: T
authors.lastname: Jomgeow
authors.forename: Mitsugu
authors.initials: M
authors.lastname: Sekimoto
authors.forename: Riichiro
authors.initials: R
authors.lastname: Nezu
authors.forename: Yukinobu
authors.initials: Y
authors.lastname: Yoshikawa
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Inoue
authors.forename: Naoki
authors.initials: N
authors.lastname: Hosen
authors.forename: Manabu
authors.initials: M
authors.lastname: Kawakami
authors.forename: Akihiro
authors.initials: A
authors.lastname: Tsuboi
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Oka
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Ogawa
authors.forename: Shigeo
authors.initials: S
authors.lastname: Souda
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Aozasa
authors.forename: Morito
authors.initials: M
authors.lastname: Monden
authors.forename: Haruo
authors.initials: H
authors.lastname: Sugiyama
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology; D017931:DNA Primers; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D016162:Genes, Wilms Tumor; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D012004:Rectal Neoplasms / Q000235:genetics* / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D025721:WT1 Proteins / Q000235:genetics*
chemical_list: D017931:DNA Primers; D025721:WT1 Proteins