Increase in adrenomedullin gene expression in the left atrium and ventricle in the two-kidney one-clip renovascular hypertensive rats.
The 2-kidney 1-clip rat model was set up by clipping the left renal artery. At 5 weeks after clipping, there was an increase in preproadrenomedullin mRNA levels in both the left atria and the left ventricle. Adrenomedullin (AM) contents, however, increased in the left ventricle but decreased in the left atrium. These changes were not observed at 2 weeks after clipping. There were no changes in AM or preproadrenomedullin mRNA levels in the thoracic aorta and the mesenteric artery, and in plasma AM levels at 2 weeks or 5 weeks after clipping. We concluded that there was an increase in the secretion of AM in the left ventricle and the left atria in the 5 week renovascular hypertensive rat. The lack of change in plasma AM level suggests a paracrine function for the peptide in this setting.
Hwang
Isabel S S
IS
Department of Physiology, Faculty of Medicine, The University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong, China.
Cheung
Bernard M Y
BM
Tang
Fai
F
D053607:Adrenomedullin; D000818:Animals; D015870:Gene Expression / Q000502:physiology; D006321:Heart / Q000502:physiology*; D006325:Heart Atria / Q000378:metabolism; D006352:Heart Ventricles / Q000378:metabolism; D006977:Hypertension, Renal / Q000503:physiopathology*; D007668:Kidney / Q000098:blood supply / Q000503:physiopathology; D008297:Male; D010455:Peptides / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D011863:Radioimmunoassay; D051381:Rats; D017207:Rats, Sprague-Dawley; D012077:Renal Artery; D013525:Surgical Instruments
D010455:Peptides; D012333:RNA, Messenger; D053607:Adrenomedullin