Protective effect of prolactin and placental lactogen on NO-induced Nb2 lymphoma cell apoptosis.
Nitric oxide (NO) is an important modulator involved in immune regulation. Here, we describe conditions under which NO-donors induce apoptosis on Nb2 lymphoma cells, as evidenced by decreased cell viability and increased hypodiploid DNA content determined by flow cytometry. In addition, DNA fragmentation typical of apoptosis was shown by agarose gel electrophoresis. This apoptosis was accompanied by a significant increase of caspase-3-like enzymatic activity. Both ovine prolactin (oPRL) and ovine placental lactogen (oPL) exerted a protective effect on the NO-donor-induced apoptosis. Furthermore, dexamethasone (Dex)-induced cell death was also associated with caspase-3-like activity and oPL had the same potency as oPRL in its protective effect on Dex-induced apoptosis of Nb2 cells.
Fernández
M Laura
ML
Instituto de Química y Fisicoquímica Biológicas (UBA-CONICET), Facultad de Farmacia y Bioquímica, Junín 956, (1113) Buenos Aires, Argentina.
Iglesias
M Mercedes
MM
Biron
Verónica A
VA
Wolfenstein-Todel
Carlota
C
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D020169:Caspases / Q000096:biosynthesis; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D003907:Dexamethasone / Q000494:pharmacology; D004171:Diploidy; D008223:Lymphoma / Q000201:enzymology / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology*; D009569:Nitric Oxide / Q000378:metabolism*; D010928:Placental Lactogen / Q000494:pharmacology*; D011388:Prolactin / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology; D051381:Rats; D014407:Tumor Cells, Cultured
D020011:Protective Agents; D009569:Nitric Oxide; D003907:Dexamethasone; D011388:Prolactin; D010928:Placental Lactogen; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases