Vaccinia virus recombinant expressing an 87-kilodalton polyprotein that is sufficient to form astrovirus-like particles.
Human astrovirus is an important cause of acute gastroenteritis. We have generated, for the first time, a vaccinia virus recombinant expressing the astrovirus 87-kDa structural polyprotein. The results demonstrate that this expression results in the formation of virus-like particles in the absence of other astrovirus proteins and genomic RNA. The purified trypsin-activated virus-like particles strongly resemble the complete astrovirus particles.
Dalton
Rosa M
RM
Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Madrid, Spain.
Pastrana
Esperanza P
EP
Sánchez-Fauquier
Alicia
A
D001483:Base Sequence; D017931:DNA Primers; D001257:Mamastrovirus / Q000502:physiology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D011995:Recombination, Genetic; D014616:Vaccinia virus / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology*
D017931:DNA Primers; D014764:Viral Proteins