The msr/apj gene encoding the apelin receptor is an early and specific marker of the venous phenotype in the retinal vasculature.
Expression of the new G protein-coupled msr/apj receptor in the mouse embryo is restricted to the endothelial layer of the primary blood vessels and the newly forming heart (Mech. Dev. 84 (1999) 199). During development of the retinal vasculature, the msr/apj gene is not expressed throughout the vascular network, indicating a possible relationship between the localization of expression and the acquisition of arterial or venous identity (Mech. Dev. 110 (2002) 183). Here we first established that retinal expression of ephrin-B2 and its putative receptor EphB4 correlates with arterial and venous phenotype, respectively. Then we analyzed the expression pattern of msr/apj in the retinal vessels at various stages of postnatal development by in situ hybridization. In contrast to the expression of ephrin-B2 or EphB4, msr/apj transcripts can be detected as early as postnatal day P3. From P3 to P12, msr/apj expression in the vascular network is restricted to the venules and the associated capillaries. The msr/apj gene is thus an early and specific marker of the venous phenotype in the retinal vasculature.
Saint-Geniez
Magali
M
Unité INSERM U-589, CHU Rangueil, Bât. L3, 1 avenue Jean-Poulhès, 31403-Toulouse Cédex 4, France.
Argence
Claire Brand
CB
Knibiehler
Bernard
B
Audigier
Yves
Y
D000818:Animals; D000075244:Apelin Receptors; D001160:Arterioles / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis*; D036388:Ephrin-B2 / Q000378:metabolism*; D017403:In Situ Hybridization; D008024:Ligands; D051379:Mice; D008810:Mice, Inbred C57BL; D010641:Phenotype; D015347:RNA Probes; D036224:Receptor, EphB4 / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D012169:Retinal Vein / Q000254:growth & development* / Q000378:metabolism
D000075244:Apelin Receptors; C491285:Aplnr protein, mouse; D015415:Biomarkers; D036388:Ephrin-B2; D008024:Ligands; D015347:RNA Probes; D043562:Receptors, G-Protein-Coupled; D036224:Receptor, EphB4