Diterpenes from the roots of Euphorbia kansui and their in vitro effects on the cell division of Xenopus (part 2).
Four new ingenane-type diterpenes, 3-O-(2,3-dimethylbutanoyl)-13-O-dodecanoyl-20-O-acetylingenol (1), 3-O-(2,3-dimethylbutanoyl)-13-O-dodecanoyl-20-deoxyingenol (2), 3-O-(2E,4Z-decadienoyl)-20-deoxyingenol (3), and 3-O-(2E,4E-decadienoyl)-20-deoxyingenol (4), two new jatrophane-type diterpenes, kansuinins D (9) and E (10), and four known ingenane-type diterpenes were isolated from the root of Euphorbia kansui. Their structures were elucidated by spectroscopic and chemical analysis, and individual Xenopus cells at the blastular stage were cultured with the diterpenes to test for biological activity. 20-Deoxyingenol diterpenes 3 and 4 induced the greatest cell cleavage arrest (0.5 micro g/ml of each compound resulted in >75% cleavage arrest), but cell cleavage inhibitory activity became weak when C-16 had an acyl residue. In contrast, the jatrophane diterpene kansuinin D (9) showed no activity.
Wang
Li-Yan
LY
Department of Natural Products Chemistry, Shenyang Pharmaceutical Universit, Shenhe District, Shenyang 110016, People's Republic of China.
Wang
Nai-Li
NL
Yao
Xin-Sheng
XS
Miyata
Syohei
S
Kitanaka
Susumu
S
D000818:Animals; D036703:Blastula / Q000166:cytology / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D028481:Euphorbia / Q000737:chemistry*; D005260:Female; D006131:Growth Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D014982:Xenopus laevis
D004224:Diterpenes; D006131:Growth Inhibitors; D010936:Plant Extracts