[Cloning, expression and purification of neural specific HuD cDNA].
OBJECTIVE
To prokaryoticly express and purify HuD protein and its RNA recognition motifs.


METHODS
HuD protein was prokaryoticly expressed and purified by molecular cloning technology. Its biologic activity was testified by Western Blot.


RESULTS
Purified HuD protein and its RNA recognized motifs were observed.


CONCLUSIONS
The result might aid for basic research and clinical application.
Chen
Jian-hua
JH
Department of Neurology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
Liu
Xiu-qin
XQ
Guo
Yu-pu
YP
Zhang
Bin
B
Zhou
Yan
Y
Hu
Xiao-yan
XY
Yuan
Jian-gang
JG
Qiang
Bo-qin
BQ
Peng
Xiao-zhong
XZ
D000974:Antibodies, Antinuclear / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D018288:Carcinoma, Small Cell / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D051959:ELAV Proteins; D000067779:ELAV-Like Protein 4; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D009474:Neurons / Q000276:immunology; D020361:Paraneoplastic Syndromes, Nervous System / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification
D000974:Antibodies, Antinuclear; D018076:DNA, Complementary; D051959:ELAV Proteins; D000067779:ELAV-Like Protein 4; C498010:ELAVL4 protein, human; D009419:Nerve Tissue Proteins; D016601:RNA-Binding Proteins