title: [Influence of adjustment of balance of Th1/Th2 type cytokines on proliferation of glioma cells].
abstract: OBJECTIVE
  To study the influence of adjustment of balance of Th1/Th2 by external cytokines on proliferation of glioma cells.
  
  
  METHODS
  The gene expressions of Th1/Th2 type cytokines in C6, 9L, U251 and SHG44 glioma cells were detected by semiquantitative reverse transcription polymerase chain reaction (RT-PCR). After the cells were induced with IFN-gamma + IL-4 McAb and IL-4 + IFN-gamma McAb respectively, we isolated the total RNA to proceed RT-PCR again. The evaluation of cell proliferation was proceeded by MTT assay method.
  
  
  RESULTS
  There was obviously predominant expression of Th2 type cytokines in glioma cell lines (P < 0.01). The expression intensity of IFN-gamma was improved in IFN-gamma + IL-4 McAb groups and Th2 type cytokines were enhanced in IL-4 + IFN-gamma McAb groups. IFN-gamma and IL-4 McAb could cause the switch from Th2 to Th1, and could remarkably inhibit the proliferation of glioma cells in a dose-dependent way (P < 0.01). On the other hand, IL-4 and IFN-gamma McAb could strengthen the switch of Th2, and might stimulate the glioma cell growth, also in a dose-dependent way (P < 0.01).
  
  
  CONCLUSIONS
  There is a Th2 preponderance in glioma cells. IFN-gamma and IL-4 McAb could regulate the switch from Th2 to Th0 or Th1, and inhibit the proliferation of glioma cells.
authors.affiliation: Department of Neurosurgery, Second Hospital of Shandong University, Jinan 250033, China. hysh69@hotmail.com
authors.forename: Y S
authors.initials: YS
authors.lastname: Hu
authors.forename: Q L
authors.initials: QL
authors.lastname: Zhang
authors.forename: Z G
authors.initials: ZG
authors.lastname: Tian
authors.forename: H M
authors.initials: HM
authors.lastname: Wei
authors.forename: J H
authors.initials: JH
authors.lastname: Zhang
authors.forename: G
authors.initials: G
authors.lastname: Li
authors.forename: Q
authors.initials: Q
authors.lastname: Pang
authors.forename: C W
authors.initials: CW
authors.lastname: Wang
authors.forename: P
authors.initials: P
authors.lastname: Jin
authors.forename: R
authors.initials: R
authors.lastname: Sun
mesh_terms: D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D001932:Brain Neoplasms / Q000276:immunology* / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D016207:Cytokines / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects; D005910:Glioma / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology; D015847:Interleukin-4 / Q000235:genetics / Q000276:immunology; D007378:Interleukins / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000911:Antibodies, Monoclonal; D016207:Cytokines; D007378:Interleukins; D015847:Interleukin-4; D007371:Interferon-gamma