The effects of S-3-1 on lipid peroxidation and scavenging free radicals in vitro.
S-3-1 is a simplified synthetic analogue of the active principle of Salvia miltiorrhioza. Electron spin resonance spectrometry using 5,5'-dimethyl-1-pyrroline-N-oxide as a scavenger of free radicals indicated that 200 microg/ml of S-3-1 scavenged 1,1-diphenyl-2-picrylhydrazyl radicals completely. 25 microg/ml of this compound quenched 100% of superoxide anion and a concentration of 250 microg/ml of S-3-1 quenched 63% of hydroxyl radicals. It was also shown that 3.56 mg/ml of S-3-1 could inhibit lipid peroxidation in microsome fraction from rat liver induced by FeSO4 and cysteine. These results show that S-3-1 is an effective antioxidant by scavenging free radicals.
Xiaoguang
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C
Department of Pharmacology, Institute of Materia Medica, CAMS & PUMC, Beijing 100050.
Hongyan
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L
Xiaohong
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L
Yan
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L
Rui
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H
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004578:Electron Spin Resonance Spectroscopy; D016166:Free Radical Scavengers / Q000494:pharmacology*; D017665:Hydroxyl Radical; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D013481:Superoxides
D004365:Drugs, Chinese Herbal; D016166:Free Radical Scavengers; D013481:Superoxides; D017665:Hydroxyl Radical; D008315:Malondialdehyde