[Hunting for novel protein factors in G-protein pathway with yeast two-hybrid system].
OBJECTIVE
To explore the protein factors that could interact with G beta subunit within the G protein signal transducing pathway.


METHODS
The highly sensitive protein-protein interaction system--Yeast Two-hybrid System was applied to screen the human cDNA library with constructed "Bite plasmid" containing G beta subunit gene fragment. And then the false positive test was adapted.


RESULTS
Three positive gene fragments were obtained. One codes for "Actin bundling protein". The other two are new ones and their Gene Bank accession numbers are AF288405 and AF288406 respectively.


CONCLUSIONS
It is the first time to find that among human brain tissue, G beta subunit becomes a structural or functional unit interacts with actin bundling protein and the other two unknown protein factors to activate the following pathway. This result may be important to understand the relationship between the actin cytoskeleton and G proteins.
Lu
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N
Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China.
Li
J J
JJ
Huang
B R
BR
D000199:Actins / Q000502:physiology; D002352:Carrier Proteins / Q000235:genetics* / Q000502:physiology; D044387:GTP-Binding Protein beta Subunits / Q000235:genetics* / Q000502:physiology; D019204:GTP-Binding Proteins / Q000502:physiology; D015723:Gene Library; D006801:Humans; D010957:Plasmids / Q000235:genetics*; D015398:Signal Transduction / Q000502:physiology; D020798:Two-Hybrid System Techniques; D015003:Yeasts / Q000235:genetics
D000199:Actins; D002352:Carrier Proteins; D044387:GTP-Binding Protein beta Subunits; D019204:GTP-Binding Proteins