[Arsenic trioxide induced human esophageal cancer Ec109 cell apoptosis with downregulation of c-myc gene expression].
OBJECTIVE
To study effect of downregulation of c-myc gene expression with As2O3 on inducing Ec109 cell apoptosis.


METHODS
MTT reduction assay, flowcytometric analysis, DNA gel electrophoresis, Western blot and in situ cell death detection (TUNEL).


RESULTS
The viability of the cells treated with As2O3 was decreased. The cells treated with As2O3 showed a typical apoptotic morphology and hypodiploid peak before G1 phase. DNA of treated Ec109 cells appeared a ladder pattern characteristic of apoptosis. TUNEL detection analysis also revealed the DNA fragmentation and As2O3 decreased c-myc gene expression was observed.


CONCLUSIONS
As2O3 can induce Ec109 cell apoptosis with downregulation of c-myc gene expression.
Deng
Y
Y
National Laboratory of Molecular Oncology, Cancer Institute, CAMS, PUMC, Beijing 100021, China.
Lin
C
C
Zhang
X
X
Chen
D
D
Xiao
P
P
Wu
M
M
D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000008:administration & dosage / Q000494:pharmacology*; D015153:Blotting, Western; D015536:Down-Regulation / Q000187:drug effects*; D004938:Esophageal Neoplasms / Q000235:genetics / Q000473:pathology*; D015870:Gene Expression; D016259:Genes, myc / Q000235:genetics*; D006801:Humans; D020287:In Situ Nick-End Labeling; D010087:Oxides / Q000008:administration & dosage / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D001152:Arsenicals; D010087:Oxides; D000077237:Arsenic Trioxide