RNA LEGO: magnesium-dependent assembly of RNA building blocks through loop-loop interactions.
We describe the construction of nano-molecular assemblies using RNA building blocks the human immunodeficiency virus type 1 (HIV-1) dimerization initiation site (DIS) RNA, that forms stable base pairing through a magnesium-dependent loop-loop interaction ("kissing"). RNA building blocks containing two DIS or DIS-like hairpins connected by a two nucleotide linker self-assembled to form specific structures as observed by non-denaturing polyacrylamide gel electrophoresis (PAGE). Furthermore, observation of "real time" formation of the molecular assemblies by circular dichroism (CD) spectroscopy was attempted.
Horiya
Satoru
S
Department of Life Science, Tokyo Gakugei University, Koganei, Tokyo 184-8501, Japan.
Li
Xianglan
X
Kawai
Gota
G
Saito
Ryota
R
Katoh
Akira
A
Kobayashi
Koh
K
Harada
Kazuo
K
D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry*
D012313:RNA