Chemiluminescence inhibition assay for folic acid using flow injection analysis.
A new flow injection method for the determination of folic acid is described. A fast oxidation reaction occurred when folic acid was mixed with potassium ferricyanide generating ferrocyanide which then inhibited the chemiluminescent reaction of ferricyanide and luminol in alkaline medium. The decrease of chemiluminescence intensity was correlated with the folic acid concentration in the range 0.1-21 microg/mL; the detection limit for the assay was 0.03 microg/mL (3sigma). A complete analysis of folic acid, including sampling and washing, could be performed within 2 min with a relative standard deviation of less than 4.0%. The proposed method has been applied successfully to the determination of folic acid in pharmaceutical preparations.
Song
Zhenghua
Z
Department of Chemistry, Northwest University, Xi'an, 710069, People's Republic of China. songzhenghua@hotmail.com
Wang
Lin
L
D005292:Ferricyanides / Q000494:pharmacology; D017022:Flow Injection Analysis / Q000379:methods*; D005492:Folic Acid / Q000032:analysis* / Q000187:drug effects / Q000528:radiation effects; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents / Q000494:pharmacology; D008163:Luminescent Measurements; D008165:Luminol / Q000494:pharmacology; D008954:Models, Biological; D015394:Molecular Structure; D012972:Sodium Hydroxide / Q000494:pharmacology
D005292:Ferricyanides; D007202:Indicators and Reagents; C007931:hexacyanoferrate III; D012972:Sodium Hydroxide; D008165:Luminol; D005492:Folic Acid