Detection of NO-induced DNA lesions by the modified aldehyde reactive probe (ARP) assay.
When DNA is exposed to NO or HNO2, oxanine (Oxa) is formed as a major guanine lesion. For highly sensitive detection of Oxa using ARP, a probe molecule for DNA damage detection, the reactivity of ARP to Oxa was examined. Oxa site-specifically embedded in an oligonucleotide reacted with ARP but it took relatively long time (ca. 24 h) to completely convert Oxa to an ARP-labeled form.
Nakano
Toshiaki
T
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima 739-8526, Japan.
Terato
Hiroaki
H
Yoshioka
Yoshihiro
Y
Ohyama
Yoshihiko
Y
Kubo
Kihei
K
Ide
Hiroshi
H
D000447:Aldehydes / Q000737:chemistry*; D004249:DNA Damage / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel; D015335:Molecular Probes; D009569:Nitric Oxide / Q000502:physiology*
D000447:Aldehydes; D015335:Molecular Probes; D009569:Nitric Oxide