A bioassay for methylated galacturonan on pulp-fiber surfaces.
A colorimetric bioassay has been developed enabling separation of wood pulp qualities based on the quantity of methyl-esterified galacturonan exposed on fiber surfaces. Thermomechanical pulp (TMP) and chemi-TMP (CTMP) had, respectively, 3.1 and 0.7 microg galacturonan on the surface per mg wood pulp. The presence of galacturonan on the surface of TMP and CTMP was further confirmed by immunogold localization.
Hafrén
Jonas
J
Swedish University of Agricultural Sciences, Wood Ultrastructure Research Centre, Department of Wood Science, Box 7008, SE-750 07 Uppsala, Sweden. jonas.hafren@trv.slu.se
Daniel
Geoffrey
G
D003124:Colorimetry / Q000379:methods; D007118:Immunoassay / Q000379:methods; D008745:Methylation; D010368:Pectins / Q000032:analysis* / Q000737:chemistry; D012680:Sensitivity and Specificity; D014934:Wood
D010368:Pectins; C003181:polygalacturonic acid