In vivo preservation of steroid specificity in CWR22 xenografts having a mutated androgen receptor.
BACKGROUND
In vitro studies of CWR22 tumor cells lack steroid specificity. We sought to determine if CWR22 xenografts also lack steroid specificity.


METHODS
We injected castrated nude mice with CWR22 tumor cells (6 x 10(6) cells) and implanted Alzet osmotic pumps that delivered approximately 1 mg steroid/kg body weight/day.


RESULTS
Serum PSA levels were detectable in intact mice and castrated mice treated with testosterone (T), but not in those treated with estradiol (E(2)), progesterone (P), or flutamide (F). T maintained mean tumor weight similar to that in intact mice (P = NS). We observed no tumors in castrated mice or mice treated with E(2), P, or F, and tumor histology was consistent with weights.


CONCLUSIONS
The mutation of the androgen receptor (H874Y) that occurs in the CWR22 xenograft model of human prostate cancer does not significantly affect in vivo steroid specificity for E(2), P, or F.
Shao
Tsang C
TC
Department of Medicine, VA Medical Center and Baylor College of Medicine, Houston, Texas 77030, USA.
Li
Huiling
H
Eid
Wael
W
Ittmann
Michael
M
Unni
Emmanual
E
Cunningham
Glenn R
GR
D000726:Androgen Antagonists / Q000494:pharmacology; D000818:Animals; D004958:Estradiol / Q000494:pharmacology*; D005485:Flutamide / Q000494:pharmacology; D012739:Gonadal Steroid Hormones / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D018482:Muscle, Skeletal / Q000033:anatomy & histology; D009368:Neoplasm Transplantation; D009919:Orchiectomy; D009929:Organ Size; D011374:Progesterone / Q000494:pharmacology*; D017430:Prostate-Specific Antigen / Q000097:blood; D011471:Prostatic Neoplasms / Q000473:pathology / Q000503:physiopathology*; D011944:Receptors, Androgen / Q000235:genetics*; D012669:Seminal Vesicles / Q000033:anatomy & histology; D013739:Testosterone / Q000494:pharmacology*; D014183:Transplantation, Heterologous
D000726:Androgen Antagonists; D012739:Gonadal Steroid Hormones; D011944:Receptors, Androgen; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; D005485:Flutamide; D017430:Prostate-Specific Antigen