Synthesis and properties of oligonucleotides containing novel 2',4'-BNA analogues (2',4'-BNACOC).
Preorganization of the nucleoside into proper conformation is one of the most promising approaches to develop the oligonucleotides strongly interacting with nucleic acid targets in a sequence-specific manner. We designed and synthesized the 2',4'-BNACOC monomer as a novel bridged nucleic acid analogue possessing a fixed N-type sugar conformation, and also successfully achieved its incorporation into oligonucleotides. The 2',4'-BNACOC modified oligonucleotides were found to have selective and strong binding-affinity for complementary RNA rather than DNA, and to show an excellent nuclease resistance ability.
Hari
Yoshiyuki
Y
Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
Osaki
Tomohisa
T
Eguchi
Ken
K
Obika
Satoshi
S
Imanishi
Takeshi
T
D001483:Base Sequence; D009696:Nucleic Acids / Q000737:chemistry*; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*
D009696:Nucleic Acids; D009841:Oligonucleotides