[Effects of asbestos on P53 protein expression in V79 cells].
OBJECTIVE
To explore the effects of physical and chemical properties of asbestos on mutagenesis/carcinogenesis.


METHODS
The P53 protein expression in V79 cells exposed to asbestos suspension and filtrate was studied using immunohistochemical method.


RESULTS
After asbestos suspension and filtrate treatment of V79 cells, the value of hue for indicating P53 protein expression was significantly lowered, and such abnormal expression was especially observed in binucleate cells and polykaryocytes.


CONCLUSIONS
The above data demonstrated that abnormal accumulation of P53 protein in V79 cells was related to asbestos exposure and that asbestos suspension and filtrate had even greater effect on the cells at the segmentation period.
Liu
Lingyi
L
Department of Hygiene Toxicology, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Hu
Yuhua
Y
Pang
Dingguo
D
Peng
Wenzhen
W
Liu
Yuqing
Y
D000818:Animals; D001194:Asbestos / Q000633:toxicity*; D002273:Carcinogens / Q000633:toxicity*; D002478:Cells, Cultured; D006224:Cricetinae; D005347:Fibroblasts / Q000166:cytology; D007150:Immunohistochemistry; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis* / Q000235:genetics
D002273:Carcinogens; D016159:Tumor Suppressor Protein p53; D001194:Asbestos