[A study on the protective efficacy of yisheng injection against IRI in pig renal-transplant model and the mechanism therein involved].
OBJECTIVE
To inquire into the mechanism of the protective efficacy of Yisheng injection against ischemia/reperfusion injury(IRI) in pig renal-transplant model.


METHODS
A total of 24 Chinese Sichuan White Pigs (SWPs) (2-3 months old, 17.5 +/- 4.6 kg, with clear family background) were used. 6 pairs of SWPs with better results of tissue typing were allocated to the group of renal allotransplantation. The other SWPs were subjected to renal autotransplantation. Yisheng injection was added into the preservative fluid and injected intravenously after transplantation in the test groups against IRI. We detected the SWPs' liver and kidney functions and serum superoxide dismutase(SOD) and malondialdehyde(MDA) concentrations after transplantation.


RESULTS
Serum creatinine and urea levels increased in 2 days after operation and then returned to normal in both groups except the pigs suffering from acute tubular necrosis or acute rejection. The concentrations of serum MDA, SOD began to increase 2 days after operation (P < 0.05). Less increase of serum MDA, less decrease of NO and more increase of SOD in test groups were detected, compared with those in control groups (P < 0.05).


CONCLUSIONS
Yisheng injection has protective efficacy against IRI and hence may have an application foreground.
Li
Ping
P
Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Zhang
Lin
L
Peng
Zhiying
Z
Yu
Ting
T
Huang
Hengjian
H
Cheng
Jingqiu
J
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007668:Kidney / Q000098:blood supply*; D016030:Kidney Transplantation; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D015427:Reperfusion Injury / Q000378:metabolism* / Q000517:prevention & control; D013482:Superoxide Dismutase / Q000378:metabolism; D013552:Swine
D004365:Drugs, Chinese Herbal; D008315:Malondialdehyde; D013482:Superoxide Dismutase