[Growth inhibition of human nasopharyngeal carcinoma cell CNE-2L2 caused by suppression of 6A8 alpha-mannosidase expression].
OBJECTIVE
To study the relationship between 6A8 alpha-mannosidase expression and growth of CNE-2L2 cells, a human nasopharyngeal carcinoma cell line.


METHODS
Recombinant adeno-associated virus vector (rAAV) was used as a mediator to transfer an antisense or a sense fragment of 6A8 cDNA into CNE-2L2 cells. 6A8 alpha-mannosidase expression was detected by means of mAb 6A8a staining, alpha-mannosidase activity assay and ConA binding test. Cell growth was examined by means of MTT and colony formation. Tumor growth at the inoculated site of the cells in nude mice was detected after 8 weeks.


RESULTS
Transduction of antisense 6A8 could reduce the expression of 6A8 alpha-mannosidase. In comparison to those in controls, the wild type, the mock-transduced and the sense 6A8-transduced cells, the MTT value, the colony number formed and the tumor weight grown at the inoculated site of cells were significantly decreased in the antisense 6A8-transduced cells (P < 0.001).


CONCLUSIONS
Decreased expression of 6A8 alpha-mannosidase caused an inhibition of CNE-2L2 cell growth.
Yue
W
W
Department of Immunology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China.
Shi
G X
GX
Bai
Z L
ZL
Zhu
L P
LP
D000818:Animals; D002455:Cell Division / Q000502:physiology; D045744:Cell Line, Tumor; D016373:DNA, Antisense / Q000235:genetics; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009303:Nasopharyngeal Neoplasms / Q000201:enzymology / Q000473:pathology*; D014162:Transfection; D043323:alpha-Mannosidase / Q000096:biosynthesis* / Q000235:genetics
D016373:DNA, Antisense; D043323:alpha-Mannosidase