[Observation on the amount of oxygen consumption by Oncomelania hupensis under low temperature].
OBJECTIVE
To observe hibernation phenomena of Oncomelanai hupensis and explore the way of inducing the hibernation in laboratory.


METHODS
Snails, O. hupensis hupensis, were collected from marshland of Jiangsu. The snail hibernation was induced by the way of cultivation at a mimic natural environment in the laboratory with gradually changing temperature. The amount of oxygen consumed by snails was tested by iodine titration, and their hibernation was tested by pin puncture followed by warm water.


RESULTS
There was no significant difference on the rate of snail hibernation when the temperature was reduced by 1 degree C per 24 hrs and by 1 degree C per 48 hrs. The hibernation rate increased with the decreasing temperature. There was a significant regression relationship between hibernation rate and temperature with R2 = 0.967 (F = 207.72, P < 0.01). The temperature for 50% snails at hibernation (ET50) was at 5.87 degrees C with 95% confidence limit of 5.32-6.23 degrees C. The amount of oxygen consumed by snails declined with reduced temperature, there was a significant regression relationship between oxygen consumption and temperature with R2 = 0.963 (F = 182.18, P < 0.01). A significant regression relationship was also shown between oxygen consumption and hibernation rate (R2 = 0.916, F = 75.88, P < 0.01).


CONCLUSIONS
Snail hibernation can be induced by the way of gradually decreasing temperature, and pin puncture or warm water resuscitation can be used to determine the status of snail hibernation.
Hong
Qing-biao
QB
Jiangsu Institute of Parasitic Diseases, Wuxi 214064.
Zhou
Xiao-nong
XN
Hang
De-rong
DR
Sun
Le-ping
LP
Yang
Guo-jing
GJ
Huang
Yi-xin
YX
Yang
Kun
K
D000818:Animals; D010101:Oxygen Consumption; D012908:Snails / Q000502:physiology*; D013696:Temperature