Experimental study on the segmental perfusion and preservation of spleen.
OBJECTIVE
Spleen transplanlation has developed to be an effective strategy for hemophilia A. But it has not been reported up to date that which kind of established solutions is most suitable for perfusion and preservation of spleen. This study aimed to establish some experiences with the comparison among Hartmann's solution, Collins' solution and WMO-I solution, in order to instruct the clinical spleen transplantation.


METHODS
After the splenic artery and vein were dissociated clearly, three kinds of perfusion solutions began to perfuse the corresponding segments of spleen with a randomized sequence. When the efferent fluids from the splenic vein became clear, the perfused spleen segments were preserved for different durations with the same perfusing solution to calculate the survival rate of splencocyte (SRS) and were examined with light and electron microscopy.


RESULTS
Among the three solutions, SRS with WMO-I solution was significantly higher than those of the other two (P<0.001). The perfused spleen with WMO-I solution showed the slightest morphological changes and a significant longer preservation duration than those with the other two (P<0.05 or P<0.01).


CONCLUSIONS
Among the three solutions, WMO-I solution was most suitable for perfusion and preservation of spleen.
Limin
H
H
Department of Surgery, First Affiliated Hospital of Harbin Medical University, Harbin 150001.
Hongchi
J
J
Wenjie
D
D
Haiquan
Q
Q
Jun
X
X
D000818:Animals; D002470:Cell Survival; D004285:Dogs; D006982:Hypertonic Solutions; D007552:Isotonic Solutions; D009926:Organ Preservation; D019188:Organ Preservation Solutions; D010477:Perfusion; D011897:Random Allocation; D000077325:Ringer's Lactate; D013154:Spleen; D020858:Tissue and Organ Harvesting
C024102:Collins' solution; D006982:Hypertonic Solutions; D007552:Isotonic Solutions; D019188:Organ Preservation Solutions; D000077325:Ringer's Lactate