[Loach spermatozoa transfer foreign DNA, which expression is discovered in the early development stages].
The transfer of plasmid pcDNA3-lacZ by electrotrasfected sperm cells into loach (Misgurnus fossilis L.) ova has been studied. The lacZ gene has been found to express in 3- to 5-day-old prelarvae.
Andreeva
L E
LE
Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182 Russia. andreevalevg@hotmail.com
Sleptsova
L A
LA
Grigorenko
A P
AP
Gavriushkin
A V
AV
Kuznetsov
A V
AV
D000818:Animals; D003531:Cypriniformes / Q000196:embryology / Q000235:genetics / Q000254:growth & development*; D018274:Electroporation / Q000379:methods; D004625:Embryo, Nonmammalian; D005260:Female; D018507:Gene Expression Regulation, Developmental; D022761:Gene Transfer, Horizontal; D007763:Lac Operon; D008297:Male; D013094:Spermatozoa / Q000502:physiology*