[Study on polymethacrylate nanoparticles as delivery system of antisense oligodeoxynucleotides].
OBJECTIVE
To investigate the possibility of polymethacrylate nanoparticles (NP) for antisense oligodeoxynucleotides delivery system.


METHODS
The nanoparticles were prepared by evaporating ethenol solution containing Eudragit RL100 or RS100, and then mixtured with oligonucleotides. The morphology and size were investigated by a transmission electron microscope and Mastersizer particle characterization systems, and the cytotoxicity was evaluated by Trypan Blue staining and hemolysis test. The flow cytometer was used to determine the uptake of fluorescence-labelled oligodeoxynucleotides.


RESULTS
The morphology of nanoparticles showed spherical and orderly, the average diameter was about 127 nm, and almost the antisense oligodeoxynucleotides (ODN) were loaded when NP: ODN was 6.6. The uptake of ODN was significantly increased when loaded by nanoparticles, which well depended on the nanoparticles concentration. Meanwhile, slightly cytotoxicity was observed when high dose of nanoparticles was used.


CONCLUSIONS
The polymethacrylate nanoparticles appeared to be a promising vehicle for gene delivery.
Wang
Wen-xi
WX
Department of Pharmaceutics, School of Pharmacy, Zhejiang University, Hangzhou 310031, China.
Chen
Hai-liang
HL
Liang
Wen-quan
WQ
D000180:Acrylic Resins / Q000737:chemistry* / Q000633:toxicity; D000818:Animals; D004337:Drug Carriers; D016503:Drug Delivery Systems; D006461:Hemolysis / Q000187:drug effects; D036103:Nanotechnology; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage*; D010316:Particle Size; D013678:Technology, Pharmaceutical / Q000379:methods
D000180:Acrylic Resins; D004337:Drug Carriers; D016376:Oligonucleotides, Antisense; C050528:Eudragit RS