Regulated expression of the Escherichia coli dam gene.
Regulated expression of the Escherichia coli dam gene has been achieved with the araBAD promoter lacking a ribosome binding site. Cultures of dam mutants containing plasmid pMQ430 show no detectable methylation in the absence of arabinose and complete methylation in its presence. Dam methyltransferase is a substrate for the Lon protease.
Calmann
Melissa A
MA
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA.
Marinus
M G
MG
D049069:ATP-Dependent Proteases; D001089:Arabinose / Q000378:metabolism; D003470:Culture Media; D019175:DNA Methylation; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D006360:Heat-Shock Proteins / Q000378:metabolism; D009154:Mutation; D011401:Promoter Regions, Genetic; D049070:Protease La; D012697:Serine Endopeptidases / Q000378:metabolism; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000235:genetics* / Q000378:metabolism*; D014158:Transcription, Genetic
D003470:Culture Media; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; D001089:Arabinose; C058693:Dam methyltransferase; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); C499294:dam protein, E coli; D049069:ATP-Dependent Proteases; D012697:Serine Endopeptidases; C483567:Lon protein, E coli; D049070:Protease La