Design of a molecular beacon PNA.
We have designed a novel dual-labeled PNA oligomer, having both a fluorescent dye and a quencher, by utilizing key compounds 1 and 2. We showed that the designed dual-labeled PNA oligomer works as a molecular beacon PNA. We also investigated the optimization of a stem-loop structure which can supersensitize the function as a molecular beacon PNA.
Kitagawa
Fumihiko
F
Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda Chiba 278-8510, Japan.
Ohori
Youji
Y
Ikeda
Hisafumi
H
Fujimori
Humihiro
H
Murakami
Yasuhumi
Y
Nakamura
Yushin
Y
D001704:Biopolymers; D015195:Drug Design; D005456:Fluorescent Dyes / Q000737:chemistry; D020135:Peptide Nucleic Acids / Q000737:chemistry*
D001704:Biopolymers; D005456:Fluorescent Dyes; D020135:Peptide Nucleic Acids