title: Novel polyamine coating providing non-covalent deactivation and reversed electroosmotic flow of fused-silica capillaries for capillary electrophoresis.
abstract: A new polycationic coating for use in capillary electrophoresis has been developed that enables chemical modification of fused-silica capillary surfaces for analysis of compounds like basic proteins. The cationic polyamine, containing short aliphatic blocks of combined 2 and 3-carbon length, was physically adsorbed onto the negatively charged fused-silica surface through ionic interaction by flushing the capillary with a polyamine solution, followed by a self-stabilization step. The polyamine coated capillaries generated an anodal electroosmotic flow that was independent of pH in the investigated range of pH 4-8. The capillary performance was demonstrated by fast separations of basic proteins with peak efficiencies in the range of 265,000-584,000 plates.
authors.affiliation: Department of Analytical Chemistry, Uppsala University, Box 599, SE-751 24 Uppsala, Sweden.
authors.forename: Emilia
authors.initials: E
authors.lastname: Hardenborg
authors.forename: Aida
authors.initials: A
authors.lastname: Zuberovic
authors.forename: Sara
authors.initials: S
authors.lastname: Ullsten
authors.forename: Lennart
authors.initials: L
authors.lastname: Söderberg
authors.forename: Eva
authors.initials: E
authors.lastname: Heldin
authors.forename: Karin E
authors.initials: KE
authors.lastname: Markides
mesh_terms: D000327:Adsorption; D002412:Cations; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D009995:Osmosis; D011073:Polyamines; D011506:Proteins / Q000302:isolation & purification; D012822:Silicon Dioxide; D055672:Static Electricity; D013499:Surface Properties
chemical_list: D002412:Cations; D011073:Polyamines; D011506:Proteins; D012822:Silicon Dioxide