title: Methylprednisolone increases neuronal apoptosis during autoimmune CNS inflammation by inhibition of an endogenous neuroprotective pathway.
abstract: Optic neuritis is one of the most common clinical manifestations of multiple sclerosis (MS), a chronic inflammatory disease of the CNS. High-dosage methylprednisolone treatment has been established as the standard therapy of acute inflammation of the optic nerve (ON). The rationale for corticosteroid treatment lies in the antiinflammatory and immunosuppressive properties of these drugs, as shown in experimental autoimmune encephalomyelitis (EAE), the animal model of MS. To investigate the influence of methylprednisolone therapy on the survival of retinal ganglion cells (RGCs), the neurons that form the axons of the ON, we used a rat model of myelin oligodendrocyte glycoprotein (MOG)-induced EAE. Optic neuritis was diagnosed by recording visual evoked potentials, and RGC function was monitored by measuring electroretinograms. Methylprednisolone treatment significantly increased RGC apoptosis during MOG-EAE. By Western blot analysis, we identified the underlying molecular mechanism: a suppression of mitogen-activated protein kinase (MAPK) phosphorylation, which is a key event in an endogenous neuroprotective pathway. The methylprednisolone-induced inhibition of MAPK phosphorylation was calcium dependent. Hence, we provide evidence for negative effects of steroid treatment on neuronal survival during chronic inflammatory autoimmune disease of the CNS, which should result in a reevaluation of the current therapy regimen.
authors.affiliation: Neurologische Universitätsklinik, 37075 Göttingen, Germany. rdiem@gwdg.de.
authors.forename: Ricarda
authors.initials: R
authors.lastname: Diem
authors.forename: Muriel
authors.initials: M
authors.lastname: Hobom
authors.forename: Katharina
authors.initials: K
authors.lastname: Maier
authors.forename: Robert
authors.initials: R
authors.lastname: Weissert
authors.forename: Maria K
authors.initials: MK
authors.lastname: Storch
authors.forename: Roman
authors.initials: R
authors.lastname: Meyer
authors.forename: Mathias
authors.initials: M
authors.lastname: Bähr
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000378:metabolism; D002452:Cell Count; D002470:Cell Survival / Q000187:drug effects; D002490:Central Nervous System / Q000187:drug effects* / Q000473:pathology; D004195:Disease Models, Animal; D004596:Electroretinography; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005074:Evoked Potentials, Visual / Q000187:drug effects; D005260:Female; D008775:Methylprednisolone / Q000009:adverse effects* / Q000494:pharmacology; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D063308:Myelin-Oligodendrocyte Glycoprotein; D009474:Neurons / Q000187:drug effects* / Q000473:pathology; D009902:Optic Neuritis / Q000139:chemically induced / Q000188:drug therapy / Q000503:physiopathology; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats; D011914:Rats, Inbred BN; D012165:Retinal Ganglion Cells / Q000187:drug effects / Q000473:pathology; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D015220:Calcium Channels; D004791:Enzyme Inhibitors; C571328:Mog protein, rat; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D063308:Myelin-Oligodendrocyte Glycoprotein; D020928:Mitogen-Activated Protein Kinases; D002118:Calcium; D008775:Methylprednisolone