Physicochemical analysis of nucleobase-metal interactions.
It is important to know what kinds of chemical bonds are formed between metal ions and nucleic acids. We have studied metal-nucleobase interactions, by using NMR spectroscopy in solution. For the accurate understanding, we tried measurement at low temperature below -30 degrees C. To avoid freezing of the solution, we have extensively searched solution conditions which is suitable for measurements at low temperature, and found several possible conditions. One was the condition which contained H2O and cryoprotectant at low pH for guanosine-5'-monophosphate--Cd(II), system, the other was the mixed solvent of dimethyl sulfoxide and N,N-dimethylformamide for guanosine--Cd(II) system.
Tanaka
Yoshiyuki
Y
Department of Molecular Transformation, Guraduate School of Pharmaceutical Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai, Miyagi 980-8578, Japan.
Toyozawa
Atsushi
A
Kondo
Yoshinori
Y
D003080:Cold Temperature; D003300:Copper / Q000737:chemistry*; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D004126:Dimethylformamide / Q000737:chemistry; D006151:Guanosine / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular
D006151:Guanosine; D003300:Copper; D004126:Dimethylformamide; D004121:Dimethyl Sulfoxide