Vaccination against Fasciola hepatica infection using a Schistosoma mansoni defined recombinant antigen, Sm14.
Fasciola hepatica is the causative agent of fasciolosis in many areas in America, Europe, Africa, Asia and Australia. There is an urgent need for improved methods to control the parasite's transmission. We describe the use of an experimental vaccine based on a recombinant antigen cloned from another parasite, Schistosoma mansoni (Sm14), that induces high levels of cross protection in mice against both S. mansoni and F. hepatica. Sheep and mice vaccinated with Sm14 were significantly protected against challenge infection with metacercariae of Fasciola hepatica and were completely free of the histopathological hepatic damage related to liver fluke infection. The vaccine will provide a valuable new tool to aid in transmission control of this economically important disease.
Almeida
Marília S
MS
Universidade Federal de Santa Catarina - MIP/CCB, Campus Universitário, Trindade, 88010-970, Florianópolis, - SC, Brazil.
Torloni
Humberto
H
Lee-Ho
Paulo
P
Vilar
Monica M
MM
Thaumaturgo
Nilton
N
Simpson
Andrew J G
AJ
Tendler
Miriam
M
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D002352:Carrier Proteins / Q000008:administration & dosage* / Q000276:immunology; D005210:Fasciola hepatica / Q000276:immunology*; D005211:Fascioliasis / Q000276:immunology / Q000517:prevention & control*; D050612:Fatty Acid Transport Proteins; D015801:Helminth Proteins / Q000008:administration & dosage* / Q000276:immunology; D007110:Immunity, Active; D008099:Liver / Q000473:pathology; D026901:Membrane Transport Proteins; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D012550:Schistosoma mansoni / Q000276:immunology*; D012756:Sheep; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics
D000947:Antigens, Helminth; D002352:Carrier Proteins; D050612:Fatty Acid Transport Proteins; D015801:Helminth Proteins; D026901:Membrane Transport Proteins; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; C068709:SM14 protein, Schistosoma mansoni