Novel polyamine coating providing non-covalent deactivation and reversed electroosmotic flow of fused-silica capillaries for capillary electrophoresis.
A new polycationic coating for use in capillary electrophoresis has been developed that enables chemical modification of fused-silica capillary surfaces for analysis of compounds like basic proteins. The cationic polyamine, containing short aliphatic blocks of combined 2 and 3-carbon length, was physically adsorbed onto the negatively charged fused-silica surface through ionic interaction by flushing the capillary with a polyamine solution, followed by a self-stabilization step. The polyamine coated capillaries generated an anodal electroosmotic flow that was independent of pH in the investigated range of pH 4-8. The capillary performance was demonstrated by fast separations of basic proteins with peak efficiencies in the range of 265,000-584,000 plates.
Hardenborg
Emilia
E
Department of Analytical Chemistry, Uppsala University, Box 599, SE-751 24 Uppsala, Sweden.
Zuberovic
Aida
A
Ullsten
Sara
S
Söderberg
Lennart
L
Heldin
Eva
E
Markides
Karin E
KE
D000327:Adsorption; D002412:Cations; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D009995:Osmosis; D011073:Polyamines; D011506:Proteins / Q000302:isolation & purification; D012822:Silicon Dioxide; D055672:Static Electricity; D013499:Surface Properties
D002412:Cations; D011073:Polyamines; D011506:Proteins; D012822:Silicon Dioxide