title: Molecular characterisation of Neoparamoeba strains isolated from gills of Scophthalmus maximus.
abstract: Small subunit ribosomal RNA gene sequences were determined for 5 amoeba strains of the genus Neoparamoeba Page, 1987 that were isolated from gills of Scophthalmus maximus (Linnaeus, 1758). Phylogenetic analyses revealed that 2 of 5 morphologically indistinguishable strains clustered with 6 strains identified previously as N. pemaquidensis (Page, 1970). Three strains branched as a clade separated from N. pemaquidenis and N. aestuarina (Page, 1970) clades. Our analyses suggest that these 3 strains could be representatives of an independent species. In a more comprehensive eukaryotic tree, strains belonging to Neoparamoeba spp. formed a monophyletic group with a sister-group relationship to Vannella anglica Page, 1980. They did not cluster with Gymnamoebae of the families Hartmannellidae, Flabellulidae, Leptomyxidae or Amoebidae presently available in GenBank.
authors.affiliation: Institute of Parasitology, Academy of Sciences of the Czech Republic, Faculty of Biological Sciences, University of South Bohemia, Branisovská 31,370 05 Ceské Budĕjovice, Czech Republic.
authors.forename: Ivan
authors.initials: I
authors.lastname: Fiala
authors.forename: Iva
authors.initials: I
authors.lastname: Dyková
mesh_terms: D000562:Amebiasis / Q000469:parasitology / Q000662:veterinary*; D000656:Amoeba / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D000818:Animals; D001483:Base Sequence; D005393:Fish Diseases / Q000469:parasitology*; D005412:Flatfishes / Q000469:parasitology*; D005880:Gills / Q000469:parasitology* / Q000473:pathology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000737:chemistry; D016415:Sequence Alignment / Q000662:veterinary; D017422:Sequence Analysis, DNA
chemical_list: D012335:RNA, Ribosomal