title: Integration of distributed multi-analyzer monitoring and control in bioprocessing based on a real-time expert system.
abstract: A computer system solution for integration of a distributed bioreactor monitoring and control instrumentation on the laboratory scale is described. Bioreactors equipped with on-line analyzers for mass spectrometry, near-infrared spectroscopy, electrochemical probes and multi-array gas sensors and their respective software were networked through a real-time expert systems platform. The system allowed data transmission of more than 1800 different signals from the instrumentation, including signals from gas sensors, electrodes, spectrometer detectors, balances, flowmeters, etc., and were used for processing and carrying out a number of computational tasks such as partial least-square regression, principal component analysis, artificial neural network modelling, heuristic decision-making and adaptive control. The system was demonstrated on different cultivations/fermentations which illustrated sensor fusion control, multivariate statistical process monitoring, adaptive glucose control and adaptive multivariate control. The performance of these examples showed high operational stability and reliable function and meet typical requirements for production safety and quality.
authors.affiliation: Department of Physics and Measurement Technology, Division of Biotechnology, Linköping University, Linköping 58183, Sweden. ctcm@novozymes.com
authors.forename: Christian
authors.initials: C
authors.lastname: Cimander
authors.forename: Thomas
authors.initials: T
authors.lastname: Bachinger
authors.forename: Carl-Fredrik
authors.initials: CF
authors.lastname: Mandenius
mesh_terms: D000465:Algorithms; D019149:Bioreactors; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods*; D003205:Computing Methodologies; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005103:Expert Systems; D009862:Online Systems / Q000295:instrumentation*; D012984:Software; D018511:Systems Integration; D014159:Transducers; D014584:User-Computer Interface