title: Recognition by cellular and humoral autologous immunity in a human osteosarcoma cell line.
abstract: Because of the difficulty of developing pairs of osteosarcoma cell lines and cytotoxic T lymphocytes (CTLs), no osteosarcoma tumor antigens that are useful for antiosteosarcoma immunotherapy have yet been identified. In parallel with continuous attempts to develop such pairs from osteosarcoma, we employed serological identification using a recombinant expression cloning (SEREX) method to identify B cell-defined antigens. Consequently, a human osteosarcoma cell line, OS2000, was established from a primary osteosarcoma of a patient cured of hereditary retinoblastoma. Repetitious in vitro stimulations by OS2000 cells to the autologous peripheral T cells induced cytotoxic activity in the autologous osteosarcoma cells but not in the nontumor cells. The cytotoxicity was inhibited by anti-HLA class I monoclonal antibody. SEREX analysis revealed that autologous humoral immunity reacted to two proteins expressed in OS2000. One was the self HLA-Cw*0102 molecule, and the other was wild-type smooth muscle myosin light chain (SMMLC). However, no antigenicity of these proteins was seen versus the sera of the other patients. In conclusion, our results demonstrated the presence of host cellular and humoral immune responses to autologous osteosarcoma cells. This offered the opportunity to identify osteosarcoma antigens recognized by autologous immunity.
authors.affiliation: Department of Orthopaedic Surgery, Sapporo Medical University School of Medicine, S. 1, W. 16, Chuo-ku, 060-8543, Sapporo, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Nabeta
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kawaguchi
authors.forename: Hiroeki
authors.initials: H
authors.lastname: Sahara
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Ikeda
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Hirohashi
authors.forename: Takahiro
authors.initials: T
authors.lastname: Goroku
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Sato
authors.forename: Tomohide
authors.initials: T
authors.lastname: Tsukahara
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Torigoe
authors.forename: Takuro
authors.initials: T
authors.lastname: Wada
authors.forename: Mitsunori
authors.initials: M
authors.lastname: Kaya
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Hiraga
authors.forename: Kazuo
authors.initials: K
authors.lastname: Isu
authors.forename: Shinya
authors.initials: S
authors.lastname: Yamawaki
authors.forename: Seiichi
authors.initials: S
authors.lastname: Ishii
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Yamashita
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Sato
mesh_terms: D000917:Antibody Formation / Q000235:genetics / Q000276:immunology*; D001324:Autoantigens / Q000235:genetics / Q000276:immunology; D001859:Bone Neoplasms / Q000235:genetics / Q000276:immunology*; D045744:Cell Line, Tumor / Q000276:immunology*; D002999:Clone Cells / Q000276:immunology; D015723:Gene Library; D006801:Humans; D007111:Immunity, Cellular / Q000235:genetics / Q000276:immunology*; D066298:In Vitro Techniques; D012516:Osteosarcoma / Q000235:genetics / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D001324:Autoantigens