Identification of the mitochondrial pyruvate carrier in Saccharomyces cerevisiae.
Mitochondrial pyruvate transport is fundamental for metabolism and mediated by a specific inhibitable carrier. We have identified the yeast mitochondrial pyruvate carrier by measuring inhibitor-sensitive pyruvate uptake into mitochondria from 18 different Saccharomyces cerevisiae mutants, each lacking an unattributed member of the mitochondrial carrier family (MCF). Only mitochondria from the YIL006w deletion mutant exhibited no inhibitor-sensitive pyruvate transport, but otherwise behaved normally. YIL006w encodes a 41.9 kDa MCF member with homologous proteins present in both the human and mouse genomes.
Hildyard
John C W
JC
Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK.
Halestrap
Andrew P
AP
D000085:Acetates / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D027321:Anion Transport Proteins; D002250:Carbon Radioisotopes / Q000378:metabolism; D006801:Humans; D026901:Membrane Transport Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D051379:Mice; D033681:Mitochondrial Membrane Transport Proteins; D024101:Mitochondrial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D027501:Monocarboxylic Acid Transporters; D019289:Pyruvic Acid / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
D000085:Acetates; D027321:Anion Transport Proteins; D002250:Carbon Radioisotopes; C573715:FMP37 protein, S cerevisiae; C573718:MPC1 protein, human; C000595573:MPC1 pyruvate carrier protein, mouse; D026901:Membrane Transport Proteins; D033681:Mitochondrial Membrane Transport Proteins; D024101:Mitochondrial Proteins; D027501:Monocarboxylic Acid Transporters; D029701:Saccharomyces cerevisiae Proteins; D019289:Pyruvic Acid