Resonant Raman quantification of zeaxanthin production from Flavobacterium multivorum.
Resonant Raman scattering was used as a novel, rapid, non-destructive optical technique to measure zeaxanthin levels in Flavobacterium multivorum ATCC 55238. Culture broth, after bacterial growth for 40 h, exhibited characteristic resonance Raman vibrational modes at 1159 cm(-1) (C-C stretch) and 1525 cm(-1) (C=C stretch) upon excitation at 488 nm. A striking correlation was observed between the carotenoid level as estimated by HPLC and by resonance Raman spectroscopy.
Bhosale
Prakash
P
Department of Ophthalmology and Visual Sciences, 50 North Medical Drive, Moran Eye Center, University of Utah School of Medicine, Salt Lake City, UT 84132, USA.
Ermakov
Igor V
IV
Ermakova
Maia R
MR
Gellermann
Werner
W
Bernstein
Paul S
PS
D002851:Chromatography, High Pressure Liquid; D005417:Flavobacterium / Q000737:chemistry / Q000145:classification / Q000254:growth & development* / Q000378:metabolism*; D013045:Species Specificity; D013059:Spectrum Analysis, Raman / Q000379:methods*; D024341:Xanthophylls; D065146:Zeaxanthins; D019207:beta Carotene / Q000031:analogs & derivatives* / Q000032:analysis* / Q000096:biosynthesis* / Q000737:chemistry
D024341:Xanthophylls; D065146:Zeaxanthins; D019207:beta Carotene