Fast drug stability determination by LC variable-parameter kinetic experiments.
Variable-parameter kinetic experiments were carried out using HPLC as analytical instrument. The hydrolysis of aspirin was followed both at variable-temperature and at variable-pH conditions. The peak areas relative to salicylic acid were processed by direct fit to a mathematical model and/or by differential method obtaining, by single experiments, the values of the apparent rate constant in the whole range of temperature and pH studied. The results, although the discontinuity of this kind of analysis, are in agreement with those obtained by constant-parameter kinetics but saving experimental time.
Alibrandi
Giuseppe
G
Dipartimento di Chimica Inorganica, Chimica Analitica e Chimica Fisica, Università di Messina, Salita Sperone 31, Villaggio S. Agata, 98166 Messina, Italy. alibrandi@chem.unime.it
Coppolino
Salvatore
S
D'Aliberti
Santi
S
Ficarra
Rita
R
Micali
Norberto
N
Villari
Antonino
A
D001241:Aspirin / Q000032:analysis / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods; D004355:Drug Stability; D013678:Technology, Pharmaceutical / Q000379:methods*
D001241:Aspirin