[Arsenite-induced lipid peroxidation in Saccharomyces cerevisiae].
The ability of sodium arsenite at concentrations of 10(-2), 10(-4), and 10(-6) M to induce lipid peroxidation in Saccharomyces cerevisiae cells was studied. Arsenite at the concentrations 10(-2) and 10(-4) M enhanced lipid peroxidation and inhibited the growth of yeast cells. Enhanced lipid peroxidation likely induced oxidative damage to various cellular structures, which led to suppression of the metabolic activity of cells. Arsenite at the concentration 10(-6) M did not activate lipid peroxidation in cells. All of the tested arsenite concentrations inhibited the activity of alpha-ketoglutarate dehydrogenase and pyruvate dehydrogenase in cells. The inference is made that the toxicity of arsenite may be related to its stimulating effect on intracellular lipid peroxidation.
Samokhvalov
V A
VA
Department of Biochemistry and Biophysics, Saratov State University, ul. Astrakhanskaya 83, Saratov, 410026 Russia.
Museĭkina
N Iu
NIu
Mel'nikov
G V
GV
Ignatov
V V
VV
D018053:Arsenites / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007655:Ketoglutarate Dehydrogenase Complex / Q000037:antagonists & inhibitors / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D030481:Pyruvate Dehydrogenase (Lipoamide) / Q000037:antagonists & inhibitors / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D017670:Sodium Compounds / Q000494:pharmacology*
D018053:Arsenites; D004791:Enzyme Inhibitors; D017670:Sodium Compounds; C017947:sodium arsenite; D030481:Pyruvate Dehydrogenase (Lipoamide); D007655:Ketoglutarate Dehydrogenase Complex