title: New method for scanning of single-nucleotide polymorphisms (SNPs): recognition of guanine-guanine mismatches by dimeric naphthyridine.
abstract: Dimeric naphthyridine was designed and synthesized as a novel ligand that specifically binds to G-G mismatch, one of four SNP types. In the presence of dimeric naphthyridine, CD spectra of the G-G mismatch containing duplex noticeably changed, being accompanied by the induced CD at 300-350 nm, whereas no CD spectral change was observed for normal duplex. DNaseI footprinting titration indicated a selective binding of dimeric naphthyridine to the G-G mismatch with a dissociation constant of 53 nM.
authors.affiliation: Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Kyoto 606-8501, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Sando
authors.forename: K
authors.initials: K
authors.lastname: Nakatani
authors.forename: I
authors.initials: I
authors.lastname: Saito
mesh_terms: D020137:Base Pair Mismatch; D001483:Base Sequence; D001665:Binding Sites; D002942:Circular Dichroism; D004252:DNA Mutational Analysis; D006147:Guanine / Q000737:chemistry*; D009287:Naphthyridines; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry; D020641:Polymorphism, Single Nucleotide
chemical_list: D009287:Naphthyridines; D009692:Nucleic Acid Heteroduplexes; D006147:Guanine