Use of dipsticks for rapid diagnosis of cholera caused by Vibrio cholerae O1 and O139 from rectal swabs.
We evaluated the recently developed dipsticks for the rapid detection of Vibrio cholerae serotypes O1 and O139 from rectal swabs of hospitalized diarrheal patients after enrichment for 4 h in alkaline peptone water. The sensitivity and specificity of the dipsticks were above 92 and 91%, respectively. The dipsticks represent the first rapid test which has been successfully used to diagnose cholera from rectal swabs, and this would immensely improve surveillance for cholera, especially in remote settings.
Bhuiyan
N A
NA
Laboratory and Clinical Sciences Divisions, International Centre for Diarrhoeal Diseases Research, Bangladesh (ICDDR,B), Dhaka 1212, Bangladesh.
Qadri
Firdausi
F
Faruque
A S G
AS
Malek
M A
MA
Salam
M A
MA
Nato
Farida
F
Fournier
J M
JM
Chanteau
S
S
Sack
David A
DA
Balakrish Nair
G
G
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D002771:Cholera / Q000175:diagnosis*; D003681:Dehydration / Q000382:microbiology; D003967:Diarrhea / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D007223:Infant; D007902:Length of Stay; D008875:Middle Aged; D011934:Reagent Strips; D012007:Rectum / Q000382:microbiology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012703:Serotyping; D013048:Specimen Handling / Q000379:methods; D014734:Vibrio cholerae / Q000145:classification / Q000302:isolation & purification*
D011934:Reagent Strips