[Change of eustachian tube surfactant of guinea pigs suffering from secretory otitis media].
OBJECTIVE
To observe the change of surface active agents of eustachian tube of guinea pigs suffering from secretory otitis media, to explore the effects of surface active agents on secretory otitis media.


METHODS
To establish the animal model of guinea pigs suffering from secretory otitis media. To analyse the changes of biochemical component and activity of eustachian tube's surfactant, meanwhile; to observe surfactant change after ectogenic surfactant treatment.


RESULTS
The main components of the surfactant decrease in the model group of guinea pigs suffering from secretory otitismedia, phosphatidylcholine (PC) is (19.9 +/- 1.7)%, phosphatidylethanolamine(PE) is (36.8 +/- 2.7)%, minimum surface tension (gamma min) is (18.5 +/- 2.4) mN/m, and PC is (25.7 +/- 2.1)%, PE is (43.7 +/- 3.8)%, (P < 0.001), and gamma min is (7.6 +/- 0.8) mN/m in normal group (P < 0.001). After giving Ectogenic surfactant treatment, PC and PE increase, PC is (23.3 +/- 2.2)%, PE is (42.5 +/- 3.6)%; gamma min reduce, it is (11.8 +/- 2.3) mN/m.


CONCLUSIONS
The main biochemical components of the surfactant obviously decrease, the activity of the surfactant weakens in secretory otitis media of guinea pig. This study showed the treatment of Ectogenic surfactant is effective.
Liao
Zhi-su
ZS
Department of Otorhinolaryngology, First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China. zhishu@mail.wzptt.zj.cn
Chen
Chao
C
Du
Han
H
Wu
Xiu-ling
XL
Zhu
Jin-chang
JC
Li
Zhi-yuan
ZY
D000818:Animals; D005064:Eustachian Tube / Q000378:metabolism*; D005260:Female; D006168:Guinea Pigs; D008297:Male; D010034:Otitis Media with Effusion / Q000378:metabolism* / Q000503:physiopathology; D010713:Phosphatidylcholines / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D013501:Surface-Active Agents / Q000378:metabolism / Q000627:therapeutic use*
D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D013501:Surface-Active Agents