Humanin peptides block calcium influx of rat hippocampal neurons by altering fibrogenesis of Abeta(1-40).
Humanin peptides (including HN, HNG and other mutants) were reported previously that antagonize neurotoxicity caused by various familial Alzheimer's disease (FAD) genes and Abeta derivatives. Herein, we describe the aggregation dynamics and the representative morphological characteristics of Abeta(1-40) after different time of addition humanin peptides, which revealed that (a) the interactions of both HN and HNG with Abeta(1-40) induced quick and significant increase of light-scattering intensity, and (b) HNG also caused obvious morphological alteration from fibrillary to amorphous. In the meantime, the experiments also revealed that the interaction of HNG with Abeta(1-40) could decrease Abeta(1-40)-induced calcium rise, an initial event accompanying Abeta(1-40)-induced apoptosis of cultured neurons. Our results indicate that HNG can protect neurons by altering Abeta(1-40) morphology.
Zou
Ping
P
Single Molecule and Nano-biomedicine Laboratory, Department of Biophysics, Peking University School of Basic Medical Sciences, 100083 Beijing, China.
Ding
Yanan
Y
Sha
Yinlin
Y
Hu
Baihe
B
Nie
Songqing
S
D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000648:ultrastructure*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D006624:Hippocampus / Q000737:chemistry / Q000166:cytology / Q000187:drug effects*; D047908:Intracellular Signaling Peptides and Proteins; D017136:Ion Transport / Q000187:drug effects; D008969:Molecular Sequence Data; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D018696:Neuroprotective Agents / Q000737:chemistry / Q000494:pharmacology*; D010446:Peptide Fragments / Q000648:ultrastructure*; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology; D011506:Proteins / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D011940:Receptor Aggregation
D016229:Amyloid beta-Peptides; D047908:Intracellular Signaling Peptides and Proteins; D018696:Neuroprotective Agents; D010446:Peptide Fragments; D010455:Peptides; D011506:Proteins; C078592:amyloid beta-protein (1-40); C427211:humanin; D002118:Calcium