title: [Deletion mutation of beta-amyloid precursor protein decreases the secretion of beta-amyloid in SH-SY5Y neuroblastoma cells].
abstract: OBJECTIVE
  To explore the effect of A beta secretion lacking exons 2 and 3 of APP695 (delta APP695) on SH-SY5Y neuroblastoma cells.
  
  
  METHODS
  APP695 cDNA and delta APP695 cDNA eukaryotic expression vectors were constructed by gene recombination. Immunoprecipitation Western blotting was used to analyse the A beta secretion and MTT assay to observe the cell viability's change after being stably transfected with the plasmids by lipofectamine method.
  
  
  RESULTS
  Stable transfectants were selected in 0.6 g.L-1 G418. delta APP695 cDNA construct transfection decreased significantly the production of beta amyloid in the SH-SY5Y compared with APP695 cDNA construct. It did not affect the cell viability by MTT method.
  
  
  CONCLUSIONS
  This result suggests that the alternative splicing of APP exons 2 and 3 modulates the secretion of A beta by influencing the processing of APP.
authors.affiliation: Institute of Mental Health, Peking University, Beijing 100083, China.
authors.forename: Qinqin
authors.initials: Q
authors.lastname: Zha
authors.forename: Yan
authors.initials: Y
authors.lastname: Ruan
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Liu
authors.forename: Lianyuan
authors.initials: L
authors.lastname: Cao
authors.forename: Dai
authors.initials: D
authors.lastname: Zhang
mesh_terms: D016229:Amyloid beta-Peptides / Q000378:metabolism*; D016564:Amyloid beta-Protein Precursor / Q000235:genetics*; D015153:Blotting, Western; D005091:Exons; D017353:Gene Deletion; D006801:Humans; D010957:Plasmids; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D016229:Amyloid beta-Peptides; D016564:Amyloid beta-Protein Precursor