title: Baculoviral p35 inhibits oxidant-induced activation of mitochondrial apoptotic pathway.
abstract: In this study we report that the baculovirus p35 anti-apoptotic protein prevents cell death by quenching free radicals at a very upstream step in the apoptotic pathway. Mitochondria of activated rat peritoneal macrophages as well as Spodoptera frugiperda (Sf9) insect cells, following treatment with oxidants, H(2)O(2)/UVB irradiation, release cytochrome c followed by activation of caspase-3. Transfection of macrophages/Sf9 cells with a construct carrying the p35 gene under the CMV/HSP promoters resulted in p35 expression and consequent arrest of oxidative stress-induced apoptosis. p35 expression also inhibited cytochrome c release from the mitochondria of oxidant-exposed cells and blocked caspase-3 activation.
authors.affiliation: Laboratory of Molecular and Cellular Biology, CDFD, Hyderabad 500076, India.
authors.forename: Sudhir
authors.initials: S
authors.lastname: Sahdev
authors.forename: Tarvinder K
authors.initials: TK
authors.lastname: Taneja
authors.forename: Manjari
authors.initials: M
authors.lastname: Mohan
authors.forename: Nand K
authors.initials: NK
authors.lastname: Sah
authors.forename: Ashok K
authors.initials: AK
authors.lastname: Khar
authors.forename: Seyed E
authors.initials: SE
authors.lastname: Hasnain
authors.forename: Mohammad
authors.initials: M
authors.lastname: Athar
mesh_terms: D000818:Animals; D017209:Apoptosis; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002460:Cell Line; D002478:Cells, Cultured; D003574:Cytochrome c Group / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors*; D051034:Inhibitor of Apoptosis Proteins; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D016877:Oxidants / Q000037:antagonists & inhibitors*; D051381:Rats; D015398:Signal Transduction; D018411:Spodoptera; D014466:Ultraviolet Rays; D014764:Viral Proteins / Q000494:pharmacology*
chemical_list: D003574:Cytochrome c Group; D051034:Inhibitor of Apoptosis Proteins; D016877:Oxidants; D014764:Viral Proteins; C086430:inhibitor of apoptosis, Nucleopolyhedrovirus; D006861:Hydrogen Peroxide; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases