title: [Mutations in the D-loop region of mitochondrial DNA in ovarian tumors].
abstract: OBJECTIVE
  To investigate mutations in the D-loop region of mitochondrial DNA in ovarian tumors.
  
  
  METHODS
  The D-loop region of 25 epithelial ovarian tumors together with the adjacent normal tissues were amplified by PCR and sequenced.
  
  
  RESULTS
  Among the 25 ovarian tumors, 26 mutations were identified with the mutation rate of 32%. 19 mutations were detected in two cases of borderline carcinoma which was a special type of epithelial ovarian carcinoma. There were 6 microsatellite instabilities among the mutations and 11 new polymorphisms which were not reported previously in the GenBank.
  
  
  CONCLUSIONS
  The D-loop region of mitochondrial DNA is a highly polymorphoric and mutable region and the mutation rate is relatively high in patients with ovarian tumors.
authors.affiliation: Department of Obstetrics and Gynecology, PUMC Hospital, CAMS, PUMC, Beijing 100730, China.
authors.forename: Hong-hui
authors.initials: HH
authors.lastname: Shi
authors.forename: Liu
authors.initials: L
authors.lastname: Vincent
authors.forename: Ngan
authors.initials: N
authors.lastname: Hextan
authors.forename: Xiu-yu
authors.initials: XY
authors.lastname: Yang
mesh_terms: D018284:Cystadenocarcinoma, Serous / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D006801:Humans; D010051:Ovarian Neoplasms / Q000235:genetics*; D017354:Point Mutation; D011110:Polymorphism, Genetic
chemical_list: D004272:DNA, Mitochondrial