[Optimizing cultural conditions of measles vaccine working seed lot with orthogonal experiments].
OBJECTIVE
To improve the quality of measles vaccine and find out good combination of cultural factors of measles vaccine virus seeds.


METHODS
Orthogonal experiments were made to confirm optimized cultural factors. 5 lots of optimized measles vaccine working seed lot were prepared.


RESULTS
The titers of the optimized vaccine seed improved significantly (> or = 0.69 +/- 0.35 LgCCID50/1.0 ml, P < 0.05), compared with 5 lots of traditional working seed lot. With these seeds we trial-prepared 5 lots of lyophilized vaccines respectively, the titer and stability met the Chinese Requirements of Biological Products.


CONCLUSIONS
The orthogonal experiment is a kind of good method for optimizing cultural conditions of measles vaccine working seed lot. The yield and quality of measles vaccine can be improved in the optimized working seed lot with high titer and good stability.
Chen
Tian
T
Microbiology Department, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Chen
Pinquan
P
Jia
Wenxiang
W
Liu
Ying
Y
Jiang
Renju
R
Peng
Wei
W
Shi
Donghui
D
D003470:Culture Media; D006801:Humans; D008458:Measles Vaccine; D008459:Measles virus / Q000254:growth & development*; D014613:Vaccines, Attenuated; D014774:Virulence / Q000235:genetics; D014776:Virus Cultivation
D003470:Culture Media; D008458:Measles Vaccine; D014613:Vaccines, Attenuated