title: Airway responsiveness of rabbits after exposure to 2-octyl dodecanol.
abstract: Cooling lubricants are used in the metal industry during drilling or turning. Vapors and aerosols of these lubricants are suspected to induce airway hyperresponsiveness (AHR) in exposed workers. In a previous study the authors demonstrated that water-soluble lubricants induce AHR after acute exposure of rabbits to concentrations near the German MAK value (10 mg/m(3)). In the present investigation the influence of a fatty alcohol as special non-water-soluble cooling lubricant was examined to determine its influence on airway responsiveness (AR). The effects of an aerosolized non-water-soluble lubricant (40, 90, and 220 mg/m(3)) on AR to acetylcholine in a rabbit model were studied. Lubricant atmosphere analysis was performed with infrared spectroscopy. Before exposure, after 2 and 4 hours of application, AR to aerosols from 0.2 and 2% acetylcholine was tested. Basal airway and cardiovascular parameters as well as blood gases did not change during exposure. Lubricant aerosol concentration of 40 and 220 mg/m(3) for 4 hours did not significantly alter AR. Inhalation of 90 mg/m(3) lubricant increased contractile response to ACH significantly. In contrast to formerly investigated water-soluble cooling lubricants, the examined non-water-soluble lubricant did not increase AR in concentrations near the MAK; however, in higher concentrations a significant (p<.05) increase was obtained.
authors.affiliation: Research Institute for Occupational Medicine of the Institutions for Statutory Accident Insurance and Prevention (BGFA), Institute of the Ruhr University, Bürkle-de-la-Camp-Platz 1, D-44789 Bochum, Germany. mensing@bgfa.ruhr-uni-bochum.de
authors.forename: Thomas
authors.initials: T
authors.lastname: Mensing
authors.forename: Hajo-H
authors.initials: HH
authors.lastname: Fricke
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Marek
authors.forename: Bruno
authors.initials: B
authors.lastname: Voss
authors.forename: Thomas
authors.initials: T
authors.lastname: Brüning
authors.forename: Michael
authors.initials: M
authors.lastname: Wilhelm
mesh_terms: D000109:Acetylcholine / Q000008:administration & dosage; D000336:Aerosols; D000403:Airway Resistance; D000818:Animals; D001985:Bronchial Provocation Tests / Q000662:veterinary; D004305:Dose-Response Relationship, Drug; D005233:Fatty Alcohols / Q000633:toxicity*; D019570:Inhalation Exposure; D011817:Rabbits; D012119:Respiration / Q000187:drug effects*
chemical_list: D000336:Aerosols; D005233:Fatty Alcohols; C456972:octyl dodecanol; D000109:Acetylcholine