title: [Determination of protein concentration by the enhancement of Rayleigh light scattering of fuchsine acid].
abstract: A new Rayleigh light scattering (RLS) assay is presented in this paper. At the optimum pH = 2.72, the weak RLS of fuchsine acid can be greatly enhanced by the addition of proteins due to the interaction between protein and fuchsine acid. A new quantitative determination method for proteins has been developed. The linear range for human serum albumin is 0-4.0 mg.L-1 with detection limit of 23 micrograms.L-1. Besides high sensitivity, the method is characterized by good reproducibility, rapidity of reaction, good stability and few interfering substances. The determination results of the proteins in human serum and urine samples are very close those obtained using Biuret method, with relative stand deviation of 0.94%-4.93%.
authors.affiliation: College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China.
authors.forename: Hong-yi
authors.initials: HY
authors.lastname: Zhang
authors.forename: Bao-sheng
authors.initials: BS
authors.lastname: Liu
authors.forename: Hong-lei
authors.initials: HL
authors.lastname: Zhang
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D001557:Benzenesulfonates; D011506:Proteins / Q000032:analysis; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D012709:Serum Albumin / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: D001557:Benzenesulfonates; D011506:Proteins; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; C086337:acid fuchsin