The presence and longevity of peripherally expanded donor-derived TCRalphabeta+ mature T lymphocyte clones after allogeneic bone marrow transplantation for adult myeloid leukemias.
There are two major pathways for T-cell regeneration after allogeneic bone marrow transplantation; thymus-dependent T-cell differentiation of T-cell progenitors, and peripheral expansion of mature T cells in the graft. In order to learn to what extent the peripheral expansion of donor-derived mature T lymphocytes contributes to reconstitution of the TCRalphabeta+ T-cell repertoire after allogeneic bone marrow transplantation for adult myeloid leukemias, we pursued the fate of donor-derived T-cell clones using the amino-acid sequences of the complementarity-determining region 3 (CDR3) of the TCR-beta chain as a clonal marker. Clonal expansion of TCRalphabeta+ T lymphocytes with specific TCRBV subfamilies was identified in donor blood. Identical T-cell clones were not found in blood from recipients before transplantation. The donor-derived T-cell clones were identified in the circulating blood from recipients a few months after allogeneic bone marrow transplantation, and they remained in the blood for 18 months after transplant in two recipients, and for 56 months in one. These results suggest that the peripheral expansion of mature T lymphocytes in the graft makes a significant contribution to post-transplant T-cell regeneration during the early period of transplantation in humans, and that mature T cells can survive in recipients for several years. Further investigation will be required to explore which antigens drive the expansion of T-cell clones in donors and recipients, and the mechanisms of maintaining homeostatic balance between the thymus-dependent pathway and the peripheral expansion of mature T cells in post-transplant T-cell regeneration.
Saitoh
H
H
Department of Internal Medicine III, Akita University School of Medicine, Akita, Japan.
Hirokawa
M
M
Fujishima
N
N
Ichikawa
Y
Y
Kawabata
Y
Y
Miura
I
I
Miura
A B
AB
Matsutani
T
T
Suzuki
R
R
Sawada
K
K
D000328:Adult; D000595:Amino Acid Sequence; D001773:Blood Cells; D016026:Bone Marrow Transplantation; D002455:Cell Division; D002999:Clone Cells / Q000502:physiology; D022801:Complementarity Determining Regions / Q000235:genetics; D006085:Graft Survival; D006801:Humans; D007951:Leukemia, Myeloid / Q000628:therapy*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis* / Q000235:genetics; D012038:Regeneration; D013601:T-Lymphocytes / Q000502:physiology*; D013997:Time Factors; D018183:Transplantation Chimera; D014184:Transplantation, Homologous
D022801:Complementarity Determining Regions; D016693:Receptors, Antigen, T-Cell, alpha-beta