Mutational analysis of ras gene family in lung cancer in Thai.
H-, K- and N-ras gene mutations were analyzed in lung cancer from Thai patients. Thirteen out of 58 cases (22%) harbored the mutations. Ten cases showed K-ras gene mutations at codon 12, 1 case presented a mutation at codon 13 and another case exhibited a mutation at codon 63. Silent mutations of N-ras gene in codons 57 and 62 were seen in one patient, whilst no H-ras mutation was found in these patients. Bases change in K-ras gene were G right curved arrow T transversion (62%), G right curved arrow A transition (15%) and G right curved arrow C transition (15%), whereas T right curved arrow G transversion and A right curved arrow G transition were detected in N-ras mutant gene.
Petmitr
Songsak
S
Department of Tropical Nutrition and Food Science, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand. tmspm@mahidol.ac.th
Wongsommart
Duangdao
D
Chaksangchaichot
Panee
P
Pakeetoot
Tanett
T
Sutinont
Pantap
P
Sirivaidyapong
Patay
P
Karalak
Anant
A
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003062:Codon; D004247:DNA / Q000737:chemistry; D004252:DNA Mutational Analysis; D005260:Female; D011905:Genes, ras; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009154:Mutation; D016133:Polymerase Chain Reaction; D012907:Smoking; D013785:Thailand
D003062:Codon; D004247:DNA