[The study of effects of static magnetic field on SP-mRNA in trigeminal ganglion in rats].
OBJECTIVE
To evaluate the effect of static magnetic field on the expression of SP-mRNA in TG in rats.


METHODS
44 Wistar rats aged 6-7 weeks were put into static magnetic field and were sacrificed at 1 h, 2 h, 6 h, 12 h, 24 h, respectively. In situ hybridization method was used to evaluate the changes of SP-mRNA expression at different time point.


RESULTS
Many neurons in TG were marked with SP probes in each group, the expression of SP-mRNA increased remarkably in static magnetic field group. In this group, the percentage of SP-mRNA positive neurons in TG increased greatly in 1 h, reached its peak in 2 h, from then on, decrease of the percentage started slowly but a moderate percentage was kept until 24 h, which was thought to be enough to maintain orthodontic tooth movement. The tendency of control group was almost the same with that of experimental group. The expression of SP-mRNA was higher in experimental group within 2 h but became lower after 2 h as compared with control group, this indicated that magnetic field reduced the SP-mRNA expression and exerted restoring effect on trauma. There were significant differences between experimental groups and control group at different time points (P < 0.01).


CONCLUSIONS
The expression of SP-mRNA in TG in rats increased significantly in static magnetic field.
Chang
Xin
X
Faculty of Stomatology, China Medical University, Shenyang 110002, China.
Qin
Ke
K
Lü
Yongli
Y
D000818:Animals; D004574:Electromagnetic Fields; D005260:Female; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics / Q000528:radiation effects; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013373:Substance P / Q000096:biosynthesis* / Q000235:genetics / Q000528:radiation effects; D012668:Trigeminal Ganglion / Q000378:metabolism / Q000528:radiation effects*
D012333:RNA, Messenger; D013373:Substance P