title: In-situ near infrared spectroscopy to monitor key analytes in mammalian cell cultivation.
abstract: The use of in-situ near infrared spectroscopy (NIRS) as a tool for monitoring four key analytes in a CHO-K1 animal cell culture was investigated. Previous work using on-line NIRS to monitor bioprocesses has involved its application ex-situ where the analyzer is physically outside the fermentor, or to microbial bioprocesses. This novel application of NIRS to monitor analytes within an animal cell culture using a steam sterilizable in-situ fiber optic probe is very important for furthering the use of NIRS within the bioprocessing industry. The method of calibration used to develop the models involved the use of large data sets so that all likely variation in stoichiometry was incorporated within the models. Successful models for glucose, lactate, glutamine, and ammonia were built with Standard Error of Predictions (SEP's) of 0.072 (g/L), 0.0144 (g/L), 0.308 (mM), and 0.036 (mM), respectively of the total concentration range.
authors.affiliation: Strathclyde Fermentation Centre, Department of Bioscience and Biotechnology, University of Strathclyde, 204 George Street, Glasgow G1 1XW, United Kingdom. alison.arnold@strath.ac.uk
authors.forename: S Alison
authors.initials: SA
authors.lastname: Arnold
authors.forename: John
authors.initials: J
authors.lastname: Crowley
authors.forename: Nigel
authors.initials: N
authors.lastname: Woods
authors.forename: Linda M
authors.initials: LM
authors.lastname: Harvey
authors.forename: Brian
authors.initials: B
authors.lastname: McNeil
mesh_terms: D000641:Ammonia / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D019149:Bioreactors; D016466:CHO Cells; D002138:Calibration; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D003198:Computer Simulation; D006224:Cricetinae; D003412:Cricetulus; D005947:Glucose / Q000032:analysis / Q000378:metabolism*; D005973:Glutamine / Q000032:analysis / Q000378:metabolism*; D019344:Lactic Acid / Q000032:analysis / Q000378:metabolism*; D008660:Metabolism / Q000502:physiology; D008954:Models, Biological; D019265:Spectroscopy, Near-Infrared / Q000295:instrumentation / Q000379:methods*
chemical_list: D005973:Glutamine; D019344:Lactic Acid; D000641:Ammonia; D005947:Glucose