title: A comparative study of the expression of monoamine oxidase-A and -B mRNA and protein in non-CNS human tissues.
abstract: The distributions of monoamine oxidase (MAO)-A and -B proteins and mRNAs in human heart, lung, liver, duodenum, kidney and vasculature were compared using immunohistochemistry and cRNA in situ hybridisation. MAO-A and -B mRNA were detected in all tissues, to differing extents, but particularly in glomeruli, hepatocytes, and the crypts, muscularis mucosa and muscularis externa of duodenum. Renal proximal and distal tubules and loops of Henle had more intense labelling for mRNA of MAO-B than MAO-A; this was reflected in MAO protein expression. Little immunoreactivity was detected in glomeruli. Hepatocytes expressed MAO-A moderately, but MAO-B strongly. In lungs, similar moderately intense labelling for both MAO mRNAs and immunoreactivities was evident in pneumocytes, and epithelial and smooth muscle cells. Cardiomyocytes contained both MAO isoforms, but with more, albeit moderate, labelling for MAO-A. Both isoforms were expressed equally in duodenal villi, crypts, muscularis externa and mucosa; lower level expression occurred in mucosal and submucosal cells. MAO-A and -B mRNA were detected in endothelia, adventitia and media of a renal interlobular artery, but protein immunoreactivities were chiefly in the adventitia. The data reveal widespread tissue distribution of MAO mRNAs and proteins, but indicate that presence of MAO mRNAs does not invariably reflect quantitatively its protein expression.
authors.affiliation: Department of Life Sciences, The Nottingham Trent University, Clifton Lane, Nottingham, NG11 8NS, UK.
authors.forename: S D
authors.initials: SD
authors.lastname: Sivasubramaniam
authors.forename: C C
authors.initials: CC
authors.lastname: Finch
authors.forename: M J
authors.initials: MJ
authors.lastname: Rodriguez
authors.forename: N
authors.initials: N
authors.lastname: Mahy
authors.forename: E E
authors.initials: EE
authors.lastname: Billett
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001808:Blood Vessels / Q000166:cytology / Q000201:enzymology / Q000378:metabolism; D004386:Duodenum / Q000166:cytology / Q000201:enzymology / Q000378:metabolism; D005260:Female; D022781:Hepatocytes / Q000201:enzymology / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D007668:Kidney / Q000098:blood supply / Q000201:enzymology / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000201:enzymology / Q000378:metabolism; D008168:Lung / Q000166:cytology / Q000201:enzymology / Q000378:metabolism; D008297:Male; D008995:Monoamine Oxidase / Q000235:genetics / Q000378:metabolism*; D009206:Myocardium / Q000166:cytology / Q000201:enzymology / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D012077:Renal Artery / Q000201:enzymology / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D007527:Isoenzymes; D011506:Proteins; D012333:RNA, Messenger; D008995:Monoamine Oxidase