DNA binding of a basic leucine-zipper protein with novel folding domain.
DNA-binding proteins frequently utilize short alpha-helices as their critical DNA recognition elements. In this research, we have employed the structure-based design to construct a small domain that could target the specific DNA sequences recognized by the yeast transcriptional activator GCN4. The new DNA binding motif recognizes specific DNA sequences as a dimer with high affinity and specificity under the physiological conditions.
Sato
S
S
Institute of Advanced Energy, Kyoto University, Uji, Kyoto 611-0011, Japan.
Makino
K
K
Morii
T
T
D001665:Binding Sites; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002942:Circular Dichroism; D004271:DNA, Fungal / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D016350:Leucine Zippers / Q000235:genetics; D046911:Macromolecular Substances; D008840:Microfilament Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D017510:Protein Folding; D011494:Protein Kinases / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism
D002352:Carrier Proteins; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D046911:Macromolecular Substances; D008840:Microfilament Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; C026615:villin; D011494:Protein Kinases