[Effect of FGF-BP on angiogenesis in squamous cell carcinoma].
OBJECTIVE
To localize fibroblast growth factors binding protein (FGF-BP) mRNA in squamous cell carcinoma (SCC) and study the FGF-BP association with vascularity.


METHODS
Thirty-five primary squamous cell carcinoma specimens, 8 metastatic squamous cell carcinoma specimens and 7 histologically adjacent tissues were studied. The localization of FGF-BP mRNA expression was performed using in situ hybridization with 35 S-labeled human FGF-BP. Microvessels in tumor specimens were identified by von Willebrand Factor-specific immunohistochemical staining for endothelial cells.


RESULTS
In situ hybridization demonstrated that FGF-BP mRNA was overexpressed in SCC. But a specific hybridization signal for FGF-BP mRNA was not detected in normal adult muscle, larynx, tongue, and lymph node. The highest density of microvessels was observed at the invasive edge of the tumor. The mean microvessel count in FGF-BP positive SCC was significantly higher than that in FGF-BP negative tumors (P < 0.001).


CONCLUSIONS
The results demonstrated that FGF-BP is present in SCC of the head and neck. The association of FGF-BP expression with angiogenesis suggests that FGF-BP may be an angiogenic factor in SCC.
Li
Weimin
W
Division of Respiratory, Department of Internal Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Chen
Wenbin
W
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000098:blood supply*; D002352:Carrier Proteins / Q000378:metabolism*; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000098:blood supply*; D008297:Male; D008565:Membrane Proteins; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000098:blood supply; D009389:Neovascularization, Pathologic; D012333:RNA, Messenger / Q000378:metabolism
D002352:Carrier Proteins; C116274:FIBP protein, human; D008565:Membrane Proteins; D012333:RNA, Messenger