title: Hematological cell findings in bone marrow and peripheral blood of rabbits after experimental acute exposure to Loxosceles intermedia (brown spider) venom.
abstract: The purpose of this work was to find out the cellular changes occurring in bone marrow and peripheral blood after acute exposure to the venom of Loxosceles intermedia. Doses of 40 microg of venom were injected intradermally into five rabbits, and five rabbits receiving only phosphate-buffered saline (PBS) were used as controls. Bone marrow and peripheral blood samples were obtained before the envenomation and 4, 8, 12, 24 and 48 h, and 5, 10, 15, 20 and 30 days after envenomation. In bone marrow samples we assessed cellularity, nucleated red cells, megakaryocytes and neutrophils, and in peripheral blood we assessed red cells (red cell concentration, hemoglobin and hematocrit), leukocytes, neutrophils and platelets. Our objective was to find out if the venom has a direct effect on bone marrow and peripheral blood or if changes in both of them are secondary to the needs of tissues, and if there is a good correlation between histopathological and hematological findings. We found that the red cell parameters were not affected by the venom, except for nucleated red cells which decreased after venom exposure. The depression of megakaryocyte numbers and thrombocytopenia showed a strong correlation with the histopathologic changes observed in skin biopsies obtained from the rabbits. The changes in cellularity and neutrophils of bone marrow were strongly correlated with those in peripheral blood and skin. The thrombocytopenia and neutropenia in peripheral blood are due to marrow depression, which may be a consequence of an extensive migration of platelets and neutrophils to the necrotic lesion or the marrow depression may be a transitory effect of evenoming by L. intermedia.
authors.affiliation: Department of Cell Biology, Federal University of Paraná, Jardim das Américas, 81531-990, Curitiba, Paraná, Brazil.
authors.forename: Paulo H
authors.initials: PH
authors.lastname: da Silva
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Hashimoto
authors.forename: Fábio A
authors.initials: FA
authors.lastname: dos Santos
authors.forename: Oldemir C
authors.initials: OC
authors.lastname: Mangili
authors.forename: Waldemiro
authors.initials: W
authors.lastname: Gremski
authors.forename: Silvio S
authors.initials: SS
authors.lastname: Veiga
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000187:drug effects; D001854:Bone Marrow Cells / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects; D007962:Leukocytes / Q000187:drug effects; D008533:Megakaryocytes / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects; D010727:Phosphoric Diester Hydrolases / Q000494:pharmacology / Q000633:toxicity*; D011817:Rabbits / Q000097:blood*; D013111:Spider Venoms / Q000494:pharmacology / Q000633:toxicity*; D013112:Spiders / Q000737:chemistry*
chemical_list: D013111:Spider Venoms; C055161:loxosceles venom; D010727:Phosphoric Diester Hydrolases