Molecular design of hairpin polyamide possessing sequence-specific alkylation activity.
A novel type of sequence specific hairpin polyamide alkylating agents were synthesized and their alkylation activities were evaluated on linear 450 bp DNA fragment. To optimize the design of hairpin polyamide CPI-conjugates, several structural analogues were prepared. Analysis using high resolution denaturing gel electrophoresis suggests that such subtle structural differences effect the sequence specificity of the polyamides.
Narita
Akihiko
A
Division of Biofunctional Molecules, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Surugadai, Kanda, Chiyoda, Tokyo 101-0062, Japan.
Bando
Toshikazu
T
Sugiyama
Hiroshi
H
D000477:Alkylating Agents / Q000737:chemistry; D000478:Alkylation; D004247:DNA / Q000737:chemistry*; D009757:Nylons / Q000737:chemistry*
D000477:Alkylating Agents; D009757:Nylons; D004247:DNA