title: [Construction and expression of the recombinant plasmid pcDNA3.1/Ts87 of Trichinella spiralis].
abstract: OBJECTIVE
  To construct and express DNA plasmid of Trichinella spiralis.
  
  
  METHODS
  The BamHI and HindIII enzyme sites and KOZAK sequences were introduced at both ends of Ts87 gene by PCR. Ts87 gene was ligated into pcDNA3.1(+) vector with T4 ligase. The recombinant plasmid pcDNA3.1/Ts87 and plasmid pcDNA3.1 were transfected into an eukaryotic cell line COS7 through Lipofectamine, respectively. The BALB/c mice were immunized with the purified plasmid DNA pcDNA3.1/Ts87 through two routes: intramuscular injection and gene-gun injection.
  
  
  CONCLUSIONS
  The pcDNA3.1/Ts87 was expressed both in COS7 and in the BALB/c mice.
authors.affiliation: Department of Parasitology, Capital University of Medical Sciences, Beijing 100054.
authors.forename: Ya-ping
authors.initials: YP
authors.lastname: Yang
authors.forename: Xin-ping
authors.initials: XP
authors.lastname: Zhu
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Lu-ping
authors.initials: LP
authors.lastname: Zhang
authors.forename: Xu-na
authors.initials: XN
authors.lastname: Xu
authors.forename: Song
authors.initials: S
authors.lastname: Huang
authors.forename: Pascal
authors.initials: P
authors.lastname: Boireau
mesh_terms: D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D018509:DNA, Helminth / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000235:genetics* / Q000276:immunology; D011817:Rabbits; D014162:Transfection; D017160:Trichinella spiralis / Q000235:genetics* / Q000276:immunology; D019444:Vaccines, DNA
chemical_list: D018509:DNA, Helminth; D019444:Vaccines, DNA