Cardiac hypertrophy in vivo is associated with increased expression of the ribosomal gene transcription factor UBF.
The ribosomal DNA transcription-specific factor, UBF, is a key target for the regulation of ribosomal RNA synthesis and hypertrophic growth of isolated neonatal cardiomyocytes. In this study, we have examined whether UBF expression is also an important determinant of cardiac growth rates in vivo. We show that rDNA transcription, rRNA synthesis and UBF expression in left ventricular myocytes isolated from mice 1-6 weeks following transverse aortic constriction were significantly increased (2.5-3.5-fold) compared to the levels in myocytes from the left ventricle of sham-operated mice.
Brandenburger
Yves
Y
Molecular Cardiology, Baker Heart Research Institute, P.O. Box 6492, St Kilda Road Central, Melbourne, Vic., 8008, Australia.
Arthur
Jane F
JF
Woodcock
Elizabeth A
EA
Du
Xiao-Jun
XJ
Gao
Xiao-ming
XM
Autelitano
Dominic J
DJ
Rothblum
Lawrence I
LI
Hannan
Ross D
RD
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000235:genetics; D006332:Cardiomegaly / Q000209:etiology / Q000473:pathology*; D006352:Heart Ventricles / Q000473:pathology; D017379:Hypertrophy, Left Ventricular / Q000209:etiology / Q000473:pathology; D051379:Mice; D032342:Muscle Cells / Q000473:pathology; D035463:Pol1 Transcription Initiation Complex Proteins / Q000096:biosynthesis* / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D012335:RNA, Ribosomal / Q000096:biosynthesis; D014158:Transcription, Genetic; D015854:Up-Regulation
D035463:Pol1 Transcription Initiation Complex Proteins; D012333:RNA, Messenger; D012335:RNA, Ribosomal; C063844:transcription factor UBF; D009320:Atrial Natriuretic Factor