title: [Polymorphism of insulin receptor substrate-1 gene in patients with coronary heart diseases].
abstract: OBJECTIVE
  To explore the relationship between the polymorphism of insulin receptor substrate-1 (IRS-1) gene and coronary heart diseases in Chinese population.
  
  
  METHODS
  A total of 113 patients with coronary heart diseases and 194 healthy Chengdu residents with less than 82 mmol/L of TG and 6.2 mmol/L of TC in fasting serum testing were selected and compared. The genotype and allele frequency of IRS-1 gene polymorphism was assayed by polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP). The serum lipids were measured by enzymatic kits.
  
  
  RESULTS
  The GG genotype dominated in the IRS-1 gene with frequency of 0.960 and 0.985 for patients and healthy controls respectively. Difference appeared in R allele frequency (0.015 for healthy controls and 0.040 for patients, P = 0.05). Patients with genotype G/R had significantly higher level of serum TC and lower level of plasma glucose than patients with genotype G/G (P < 0.05).
  
  
  CONCLUSIONS
  IRS-1 gene polymorphism is associated with coronary heart disease in Chinese population. The G/R and G/G genotype of IRS-1 gene contributes to the increasing of serum cholesterol and plasma glucose respectively.
authors.affiliation: Laboratory of Internal Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.forename: Huai
authors.initials: H
authors.lastname: Bai
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.forename: Xian
authors.initials: X
authors.lastname: Li
authors.forename: Minghui
authors.initials: M
authors.lastname: Huang
authors.forename: Juelin
authors.initials: J
authors.lastname: Deng
authors.forename: Bingwen
authors.initials: B
authors.lastname: Liu
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D003327:Coronary Disease / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D055504:Insulin Receptor Substrate Proteins; D008297:Male; D008875:Middle Aged; D010750:Phosphoproteins / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length
chemical_list: C526292:IRS1 protein, human; D055504:Insulin Receptor Substrate Proteins; D010750:Phosphoproteins