Expression of beta2-integrin on monocytes and blood polymorphonuclear leukocytes in the periparturient period in dairy cows.
The hypothesis that an altered expression of CD11/CD18 on bovine circulating monocytes, polymorphonuclear leukocytes (PMN), or both, contributes to an increased mastitis susceptibility in periparturient cows was tested. Expression of CD18 and CD11a, -b, -c on bovine monocytes and PMN were assessed in 8 Friesian-Holstein cows by flow cytometry from 2 wk before calving to 5 wk after calving. Minor changes in adhesion molecule expression levels were detected throughout the experimental period. Compared with PMN, monocytes exhibited an expression level that was similar for CD18, higher for CD11a and CD11c, but lower for CD11b. Differences in density may reflect the relative importance of these adhesion molecules on both leukocyte types. In this study, the decreased number of milk resident macrophages and PMN observed during the periparturient period could not be attributed to changes of CD11/CD18 levels on circulating leukocytes.
Diez-Fraile
Araceli
A
Department of Physiology, Biochemistry and Biometrics, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
Duchateau
Luc
L
Meyer
Evelyne
E
Burvenich
Christian
C
D000818:Animals; D018845:CD11 Antigens / Q000097:blood / Q000378:metabolism; D040881:CD11a Antigen / Q000378:metabolism; D039481:CD11b Antigen / Q000378:metabolism; D039521:CD11c Antigen / Q000378:metabolism; D018821:CD18 Antigens / Q000097:blood / Q000378:metabolism*; D002417:Cattle / Q000097:blood / Q000276:immunology / Q000502:physiology*; D015815:Cell Adhesion Molecules; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D007774:Lactation / Q000276:immunology*; D008414:Mastitis, Bovine / Q000276:immunology* / Q000378:metabolism; D009000:Monocytes / Q000378:metabolism*; D009504:Neutrophils / Q000378:metabolism*; D049590:Postpartum Period; D011247:Pregnancy
D018845:CD11 Antigens; D040881:CD11a Antigen; D039481:CD11b Antigen; D039521:CD11c Antigen; D018821:CD18 Antigens; D015815:Cell Adhesion Molecules