title: Nitric oxide inhibits a rise of ATP-introduced cytosolic free Ca2+ concentration and release from intracellular stored Ca2+.
abstract: OBJECTIVE
  The effects of ATP-introduced a rise in cytosolic free Ca2+ concentration and inhibition of nitric oxide were investigated.
  
  
  METHODS
  Measurement of free Ca2+ ([Ca2+]i) of cultured rat tail arterial smooth muscle cells using Fura-2/AM dual excitation wavelength spectrofluorometer.
  
  
  RESULTS
  There are two components of [Ca2+]i can be evoked by ATP. One part is Ca2+ entry from Ca2+ channel and formed a plateau. The another part is a peak that released from Ca2+ store. Both of them can be inhibited by NO.
  
  
  CONCLUSIONS
  The ATP induced [Ca2+]i rise that release Ca2+ from both Insp3 and ryanochine receptors and Ca2+ entry through calcium channels. The inhibition of NO on ATP induced [Ca2+]i rise that was mediated by cGMP.
authors.affiliation: Institute of Basic Medical Sciences, CAMS & PUMC, Beijing 100005.
authors.forename: Z
authors.initials: Z
authors.lastname: Wang
authors.forename: Y
authors.initials: Y
authors.lastname: Deng
authors.forename: D
authors.initials: D
authors.lastname: Yu
authors.forename: G
authors.initials: G
authors.lastname: Bai
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002478:Cells, Cultured; D006152:Cyclic GMP / Q000031:analogs & derivatives* / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000378:metabolism*; D009569:Nitric Oxide / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002121:Calcium Channel Blockers; C016276:8-bromocyclic GMP; D009569:Nitric Oxide; D000255:Adenosine Triphosphate; D006152:Cyclic GMP; D002118:Calcium