title: DNA binding to fluorescent ruthenium species released from calcium phosphate/nanoporous silicon structures.
abstract: This work centers on an analysis of calf thymus DNA binding to emissive Ru complexes which diffuse from biocompatible calcium phosphate/nanoporous silicon films. These nanostructures were characterized by scanning electron microscopy, atomic force microscopy, energy dispersive X-ray analysis, and infrared vibrational spectroscopy. In terms of polynucleotide binding, three different systems were analyzed: (1) an aqueous solution of Ru(phen)(3)2+ (a control); (2) surface-adsorbed Ru(phen)(3)2+ onto undoped calcium phosphate/porous Si/Si in aqueous solution; (3) as-prepared and annealed Ru(phen)3(2+)-doped calcium phosphate/porous Si structures in water. For films with fluorescent Ru originally embedded throughout the film, biphasic diffusion character is found; such behavior is attributed to DNA binding to both surface-bound Ru(phen)(3)2+ and species which originate from deeper regions of the film.
authors.affiliation: Department of Chemistry, Texas Christian University, Ft. Worth, Texas, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Jeffery L
authors.initials: JL
authors.lastname: Coffer
mesh_terms: D000818:Animals; D001665:Binding Sites; D002130:Calcium Phosphates / Q000737:chemistry*; D002417:Cattle; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis* / Q000737:chemistry; D003460:Crystallization / Q000379:methods*; D004247:DNA / Q000032:analysis / Q000737:chemistry*; D004058:Diffusion; D005456:Fluorescent Dyes / Q000737:chemistry; D008567:Membranes, Artificial; D036103:Nanotechnology / Q000379:methods*; D009942:Organometallic Compounds / Q000737:chemistry*; D010618:Phenanthrolines / Q000737:chemistry*; D016062:Porosity; D013050:Spectrometry, Fluorescence / Q000379:methods; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; D005456:Fluorescent Dyes; D008567:Membranes, Artificial; D009942:Organometallic Compounds; D010618:Phenanthrolines; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; C034282:tris-(1,10-phenanthroline)ruthenium; C485838:calcium phosphate, monobasic, anhydrous; D004247:DNA; C427203:calf thymus DNA; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous