[Construction and expression of the recombinant plasmid pcDNA3.1/Ts87 of Trichinella spiralis].
OBJECTIVE
To construct and express DNA plasmid of Trichinella spiralis.


METHODS
The BamHI and HindIII enzyme sites and KOZAK sequences were introduced at both ends of Ts87 gene by PCR. Ts87 gene was ligated into pcDNA3.1(+) vector with T4 ligase. The recombinant plasmid pcDNA3.1/Ts87 and plasmid pcDNA3.1 were transfected into an eukaryotic cell line COS7 through Lipofectamine, respectively. The BALB/c mice were immunized with the purified plasmid DNA pcDNA3.1/Ts87 through two routes: intramuscular injection and gene-gun injection.


CONCLUSIONS
The pcDNA3.1/Ts87 was expressed both in COS7 and in the BALB/c mice.
Yang
Ya-ping
YP
Department of Parasitology, Capital University of Medical Sciences, Beijing 100054.
Zhu
Xin-ping
XP
Yang
Jing
J
Zhang
Lu-ping
LP
Xu
Xu-na
XN
Huang
Song
S
Boireau
Pascal
P
D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D018509:DNA, Helminth / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000235:genetics* / Q000276:immunology; D011817:Rabbits; D014162:Transfection; D017160:Trichinella spiralis / Q000235:genetics* / Q000276:immunology; D019444:Vaccines, DNA
D018509:DNA, Helminth; D019444:Vaccines, DNA