Zn finger and nuclear localization of transcription factor Sp1.
Transcription factor Sp1 is located in the nucleus of a mammalian cell and importantly related to expression of many cellular genes. In order to elucidate the nuclear localization mechanism of Sp1, various truncated fragments of Sp1 were fused to green fluorescent protein (GFP) and expressed in HeLa cell. The results show significance of the DNA binding region, especially, zinc finger (Zn finger) domain for nuclear localization of Sp1 in HeLa cell.
Kuwahara
J
J
Institute for Medicinal Resources, Faculty of Pharmaceutical Sciences, University of Tokushima, 1-78 Sho-machi, Tokushima 770-8505, Japan.
Watanabe
Y
Y
Kayasuga
T
T
Itoh
K
K
D001665:Binding Sites; D002467:Cell Nucleus / Q000378:metabolism; D049452:Green Fluorescent Proteins; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D015394:Molecular Structure; D019913:Nuclear Localization Signals / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011033:Podophyllin / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011034:Podophyllotoxin / Q000031:analogs & derivatives; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016335:Zinc Fingers / Q000235:genetics
D008164:Luminescent Proteins; D019913:Nuclear Localization Signals; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; C003170:mitopodozide; D011033:Podophyllin; D011034:Podophyllotoxin