title: [Stimulation of neuropeptide Y on heat shock protein expression in renal vascular smooth muscle and the inhibition thereon by losartan].
abstract: OBJECTIVE
  To study the effect of neuropeptide Y (NPY) on the expression of heat shock protein 70 (HSP70) in vascular smooth muscle cells (VSMCs) of renal arteries.
  
  
  METHODS
  The renal veins of SD rats were isolated and broken into pieces. The VSMCs were cultured and then divided into 3 groups, neuropeptide Y (NPY), NPY + losartan, and serum-free DMEM were added into the culture respectively. Automated MTT colorimetric microassay and quantitative immunocytochemistry were used to detect the expression of heat shock protein (HSP70) in the proliferating VSMCs.
  
  
  RESULTS
  The fluorescence intensity of labeled HPS70 in the NPY group was 1,825.10 +/- 115.55, significantly stronger than that in the control group (1595.83 +/- 186.54, P < 0.05) and the fluorescence intensity of labeled HPS70 in the NPY + losartan group (1 658.54 +/- 183.78) was not significantly different from that in the control group (P > 0.05). The MTT-OD in NPY group was 0.2626 +/- 0.0025, significantly higher than that in the control group (0.2239 +/- 0.0010, P < 0.01). and the MTT-OD in NPY + losartan group was 0.2440 +/- 0.0013, significantly lower than that in the control group (P < 0.05).
  
  
  CONCLUSIONS
  NPY stimulates the proliferation of renal VSMC, promotes the expression of HPS70, and may cause hypertension. Losartan reduces the NPY stimulation over VSMC proliferation and relevant expression of intracellular HSP70.
authors.affiliation: Department of Urology, First People's Hospital Affiliated to Guangzhou Medical College, Guangzhou 510180, China.
authors.forename: Wei-de
authors.initials: WD
authors.lastname: Zhong
authors.forename: Guang-qiao
authors.initials: GQ
authors.lastname: Zeng
authors.forename: Jian-bo
authors.initials: JB
authors.lastname: Hu
authors.forename: Yue-bin
authors.initials: YB
authors.lastname: Cai
authors.forename: Jian-kang
authors.initials: JK
authors.lastname: Liu
authors.forename: Shao-hua
authors.initials: SH
authors.lastname: Huang
authors.forename: Min-sheng
authors.initials: MS
authors.lastname: Chen
authors.forename: Hong-ai
authors.initials: HA
authors.lastname: Wei
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*; D019808:Losartan / Q000494:pharmacology*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009478:Neuropeptide Y / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012077:Renal Artery / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
chemical_list: D018840:HSP70 Heat-Shock Proteins; D009478:Neuropeptide Y; D019808:Losartan