Structure of the biological repeating unit of the O-antigen of Pseudomonas aeruginosa immunotype 4 containing both 2-acetamido-2,6-dideoxy-D-glucose and 2-acetamido-2,6-dideoxy-D-galactose.
A phosphorylated core-lipid A backbone oligosaccharide that carries a disaccharide remainder of the first O-antigen repeating unit was derived by strong alkaline degradation following mild hydrazinolysis of the lipopolysaccharide of Pseudomonas aeruginosa immunotype 4 (serogroup O-1). The structure of the oligosaccharide was determined using ESI MS and NMR spectroscopy and it was demonstrated that 2-acetamido-2,6-dideoxy-D-glucose is the first monosaccharide of the O-polysaccharide that is linked to the LPS core. These data define the structure of the biological repeating unit of the O-antigen.
Bystrova
Olga V
OV
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
Lindner
Buko
B
Moll
Herman
H
Kocharova
Nina A
NA
Knirel
Yuriy A
YA
Zähringer
Ulrich
U
Pier
Gerald B
GB
D000117:Acetylglucosamine / Q000031:analogs & derivatives* / Q000737:chemistry*; D002240:Carbohydrate Sequence; D005643:Fucose / Q000031:analogs & derivatives* / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000737:chemistry / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000276:immunology
D008070:Lipopolysaccharides; D019081:O Antigens; D005643:Fucose; C059377:N-acetylfucosamine; C070625:2-acetamido-2,6-dideoxyglucose; D000117:Acetylglucosamine