title: [Optimizing cultural conditions of measles vaccine working seed lot with orthogonal experiments].
abstract: OBJECTIVE
  To improve the quality of measles vaccine and find out good combination of cultural factors of measles vaccine virus seeds.
  
  
  METHODS
  Orthogonal experiments were made to confirm optimized cultural factors. 5 lots of optimized measles vaccine working seed lot were prepared.
  
  
  RESULTS
  The titers of the optimized vaccine seed improved significantly (> or = 0.69 +/- 0.35 LgCCID50/1.0 ml, P < 0.05), compared with 5 lots of traditional working seed lot. With these seeds we trial-prepared 5 lots of lyophilized vaccines respectively, the titer and stability met the Chinese Requirements of Biological Products.
  
  
  CONCLUSIONS
  The orthogonal experiment is a kind of good method for optimizing cultural conditions of measles vaccine working seed lot. The yield and quality of measles vaccine can be improved in the optimized working seed lot with high titer and good stability.
authors.affiliation: Microbiology Department, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Chen
authors.forename: Pinquan
authors.initials: P
authors.lastname: Chen
authors.forename: Wenxiang
authors.initials: W
authors.lastname: Jia
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Renju
authors.initials: R
authors.lastname: Jiang
authors.forename: Wei
authors.initials: W
authors.lastname: Peng
authors.forename: Donghui
authors.initials: D
authors.lastname: Shi
mesh_terms: D003470:Culture Media; D006801:Humans; D008458:Measles Vaccine; D008459:Measles virus / Q000254:growth & development*; D014613:Vaccines, Attenuated; D014774:Virulence / Q000235:genetics; D014776:Virus Cultivation
chemical_list: D003470:Culture Media; D008458:Measles Vaccine; D014613:Vaccines, Attenuated