Improved production of teicoplanin using adsorbent resin in fermentations.
OBJECTIVE
To use adsorbent resins in fermentations to eliminate toxic effects on growth, reduce feedback repression of production and assist in recovery of teicoplanin.


RESULTS
An adsorbent resin was added to the culture broth for the adsorption of teicoplanin. Amberlite XAD-16, Diaion HP-20, charcoal and silica gel were investigated as adsorbent resins. The adsorbed teicoplanin was extracted from the resin by 80% methanol after fermentation. Antibiotic activity was quantified by the disc-agar diffusion assay against Bacillus substilis, and qualitative evaluations were based on HPLC using YMC-Pack ODS-A column. Diaion HP-20 was the most effective adsorbent resin when added at a concentration of 5% (w/v) in the inoculation stage.


CONCLUSIONS
Addition of Diaion HP-20 in fermentations eliminated toxic effects on growth and reduced feedback repression of teicoplanin by adsorption. There was a 4.2-fold increase in the quantities of teicoplanin. Addition of adsorbent assisted in the recovery of teicoplanin by reducing the recovery steps.


CONCLUSIONS
The results of this study provide useful information for the production of teicoplanin, a glycopeptide antibiotic produced by Actinoplanes teicomyceticus. Addition of adsorbent in fermentation increased productivity of teicoplanin by more than five times.
Lee
J C
JC
Korea Research Institute of Bioscience & Biotechnology, Yusong, Daejon, South Korea.
Park
H R
HR
Park
D J
DJ
Lee
H B
HB
Kim
Y B
YB
Kim
C J
CJ
D000192:Actinomycetales / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D000327:Adsorption; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects; D001709:Biotechnology / Q000379:methods; D003470:Culture Media; D017077:Culture Media, Conditioned / Q000494:pharmacology; D005285:Fermentation; D007475:Ion Exchange Resins / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D011137:Polystyrenes / Q000737:chemistry*; D017334:Teicoplanin / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D003470:Culture Media; D017077:Culture Media, Conditioned; D007475:Ion Exchange Resins; D011137:Polystyrenes; C065649:Diaion HP 20; D017334:Teicoplanin