Determination of vigabatrin by capillary electrophoresis with laser-induced fluorescence detection.
A new analytical method for vigabatrin based on capillary electrophoretic separation and laser-induced fluorescence detection has been developed. 5-Carboxytetramethylrhodamine succinimidyl ester was used for precolumn derivatization of the non-fluorescent drug. Optimal separation and detection were obtained with an electrophoretic buffer of 50 mM sodium borate (pH9.5) containing 10 mM sodium dodecyl sulfate and a green He-Ne laser (excitation at 543.5 nm, emission at 589 nm). The concentration limit of detection in aqueous solution was 24 nM. Combined with a simple cleanup procedure, this method can be applied to the determination of vigabatrin in human plasma. A calibration curve ranging from 1.5 to 200 microM shown to be linear. Both the within-day and day-to-day reproducibilities and accuracies were less then 14.3% and 4.9% respectively. The limit of detection of vigabatrin in plasma was about 0.13 microM
Chang
Sarah Y
SY
Department of Applied Chemistry, Chaoyang University of Technology, County, Wufeng, Taichung, Taiwan 413. ychang@cyut.edu.tw
Lin
Wen-Chung
WC
D000927:Anticonvulsants / Q000097:blood*; D002138:Calibration; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D007834:Lasers; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods*; D020888:Vigabatrin / Q000097:blood*
D000927:Anticonvulsants; D020888:Vigabatrin