Tritium planigraphy comparative structural study of tobacco mosaic virus and its mutant with altered host specificity.
Spatial organization of wild-type (strain U1) tobacco mosaic virus (TMV) and of the temperature-sensitive TMV ts21-66 mutant was compared by tritium planigraphy. The ts21-66 mutant contains two substitutions in the coat protein (Ile21-->Thr and Asp66-->Gly) and, in contrast with U1, induces a hypersensitive response (formation of necroses) on the leaves of plants bearing a host resistance gene N' (for example Nicotiana sylvestris); TMV U1 induces systemic infection (mosaic) on the leaves of such plants. Tritium distribution along the coat protein (CP) polypeptide chain was determined after labelling of both isolated CP preparations and intact virions. In the case of the isolated low-order (3-4S) CP aggregates no reliable differences in tritium distribution between U1 and ts21-66 were found. But in labelling of the intact virions a significant difference between the wild-type and mutant CPs was observed: the N-terminal region of ts21-66 CP incorporated half the amount of tritium than the corresponding region of U1 CP. This means that in U1 virions the CP N-terminal segment is more exposed on the virion surface than in ts21-66 virions. The possibility of direct participation of the N-terminal tail of U1 CP subunits in the process of the N' hypersensitive response suppression is discussed.
Dobrov
Eugenie N
EN
A.N. Belozersky Institute of Physico-Chemical Biology and Department of Chemistry, Moscow State University, Russia. dobrov@belozersky.msu.ru
Badun
Gennadii A
GA
Lukashina
Elena V
EV
Fedorova
Nataliya V
NV
Ksenofontov
Alexander L
AL
Fedoseev
Vladimir M
VM
Baratova
Ludmila A
LA
D036022:Capsid Proteins / Q000737:chemistry; D006790:Host-Parasite Interactions / Q000235:genetics / Q000502:physiology; D009154:Mutation; D012588:Scintillation Counting; D014027:Tobacco Mosaic Virus / Q000737:chemistry* / Q000235:genetics; D014316:Tritium
D036022:Capsid Proteins; D014316:Tritium