title: Evidence of parvovirus replication in cerebral neurons of cats.
abstract: The correlation between parvovirus infections and lesions in the central nervous system other than cerebellar hypoplasia was studied in 100 cats. The animals were necropsied with a history of various diseases, one third showing typical clinical and pathomorphological signs of panleukopenia. In 18 cats polyclonal antiserum against canine parvovirus consistently labeled neurons mainly in diencephalic regions, whereas the cerebellar cortex remained negative in all cases. In situ hybridization with digoxigenin-labeled minus-sense RNA probes, hybridizing with monomer-replicative form DNA or mRNA, revealed positive signals in nuclei of several neurons of the brain, again excluding the cerebellum. PCR applied to formalin-fixed and paraffin-embedded brain tissue and intestinal tissues of the diseased cats and subsequent DNA sequence analysis yielded canine parvovirus type 2 (CPV-2)-like sequences in the central nervous system. Two aspects of these findings are intriguing: (i). parvoviruses appear to be capable of replicating in neurons, cells that are considered to be terminally differentiated and (ii). CPV-like viruses of the old antigenic type CPV-2 appear to be able to infect cats.
authors.affiliation: Institute of Pathology and Forensic Veterinary Medicine, Veterinary University of Vienna, A-1210 Vienna, Austria. angelika.url@vu-wien.ac.at
authors.forename: Angelika
authors.initials: A
authors.lastname: Url
authors.forename: Uwe
authors.initials: U
authors.lastname: Truyen
authors.forename: Barbara
authors.initials: B
authors.lastname: Rebel-Bauder
authors.forename: Herbert
authors.initials: H
authors.lastname: Weissenböck
authors.forename: Peter
authors.initials: P
authors.lastname: Schmidt
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology / Q000821:virology*; D002371:Cat Diseases / Q000175:diagnosis / Q000473:pathology / Q000821:virology; D002415:Cats; D002467:Cell Nucleus / Q000473:pathology / Q000821:virology; D017931:DNA Primers; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D008297:Male; D009474:Neurons / Q000473:pathology / Q000821:virology; D009919:Orchiectomy; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary; D017992:Parvovirus / Q000235:genetics / Q000502:physiology*; D016133:Polymerase Chain Reaction; D015347:RNA Probes; D012333:RNA, Messenger / Q000235:genetics; D014779:Virus Replication
chemical_list: D017931:DNA Primers; D004279:DNA, Viral; D015347:RNA Probes; D012333:RNA, Messenger