Baculoviral p35 inhibits oxidant-induced activation of mitochondrial apoptotic pathway.
In this study we report that the baculovirus p35 anti-apoptotic protein prevents cell death by quenching free radicals at a very upstream step in the apoptotic pathway. Mitochondria of activated rat peritoneal macrophages as well as Spodoptera frugiperda (Sf9) insect cells, following treatment with oxidants, H(2)O(2)/UVB irradiation, release cytochrome c followed by activation of caspase-3. Transfection of macrophages/Sf9 cells with a construct carrying the p35 gene under the CMV/HSP promoters resulted in p35 expression and consequent arrest of oxidative stress-induced apoptosis. p35 expression also inhibited cytochrome c release from the mitochondria of oxidant-exposed cells and blocked caspase-3 activation.
Sahdev
Sudhir
S
Laboratory of Molecular and Cellular Biology, CDFD, Hyderabad 500076, India.
Taneja
Tarvinder K
TK
Mohan
Manjari
M
Sah
Nand K
NK
Khar
Ashok K
AK
Hasnain
Seyed E
SE
Athar
Mohammad
M
D000818:Animals; D017209:Apoptosis; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002460:Cell Line; D002478:Cells, Cultured; D003574:Cytochrome c Group / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors*; D051034:Inhibitor of Apoptosis Proteins; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D016877:Oxidants / Q000037:antagonists & inhibitors*; D051381:Rats; D015398:Signal Transduction; D018411:Spodoptera; D014466:Ultraviolet Rays; D014764:Viral Proteins / Q000494:pharmacology*
D003574:Cytochrome c Group; D051034:Inhibitor of Apoptosis Proteins; D016877:Oxidants; D014764:Viral Proteins; C086430:inhibitor of apoptosis, Nucleopolyhedrovirus; D006861:Hydrogen Peroxide; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases