title: Selective effects of interleukin (IL)-15 on antifungal activity and IL-8 release by polymorphonuclear leukocytes in response to hyphae of Aspergillus species.
abstract: The effects of interleukin (IL)-15 on human polymorphonuclear leukocyte (PMNL) activity against Aspergillus fumigatus and Aspergillus flavus were investigated. Pretreatment with IL-15 for 2 h increased PMNL oxidative burst, as measured by superoxide anion (O(2)(-)) release in response to A. fumigatus (P<.05) but not to A. flavus. However, after 22-h, but not 2-h, treatment with IL-15, there was significant enhancement in PMNL-mediated hyphal damage to A. fumigatus. Furthermore, 22-h exposure to IL-15 mediated an enhanced release of IL-8 from PMNLs challenged with hyphae of A. fumigatus and A. flavus (P<.05). In contrast, IL-15 treatment did not affect the release of tumor necrosis factor-alpha from PMNLs. The selective time- and species-dependent enhancement of O(2)(-) production and hyphal damage, as well as its induction of IL-8 release, suggest that IL-15 may play an important role in the immunomodulation of host response to invasive aspergillosis.
authors.affiliation: Third Department of Pediatrics, Aristotle University of Thessaloniki, Hippokration Hospital, Thessaloniki, Greece.
authors.forename: Richard M
authors.initials: RM
authors.lastname: Winn
authors.forename: Cristina
authors.initials: C
authors.lastname: Gil-Lamaignere
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Roilides
authors.forename: Maria
authors.initials: M
authors.lastname: Simitsopoulou
authors.forename: Caron A
authors.initials: CA
authors.lastname: Lyman
authors.forename: Avgi
authors.initials: A
authors.lastname: Maloukou
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Walsh
mesh_terms: D001230:Aspergillus / Q000276:immunology*; D006801:Humans; D025301:Hyphae / Q000276:immunology*; D019409:Interleukin-15 / Q000494:pharmacology*; D016209:Interleukin-8 / Q000096:biosynthesis / Q000378:metabolism*; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology*; D016897:Respiratory Burst; D013045:Species Specificity; D013481:Superoxides / Q000378:metabolism; D013997:Time Factors
chemical_list: D019409:Interleukin-15; D016209:Interleukin-8; D013481:Superoxides