Adhesion of Porphyromonas gingivalis to cultured pocket epithelium: mono- and multi-layered.
Adhesion of bacteria to epithelial cells might be influenced by the degree of cell differentiation, as observed in the multi-layering process of epithelial cells. In the present study, the adhesion of a large group of clinical Porphyromonas gingivalis strains (n=11) to in vitro cultured mono- and multi-layers of epithelial cells was examined and compared. The tissue samples originated from 6 patients with chronic adult periodontitis. Porphyromonas gingivalis bacteria adhered more to mono-layers as opposed to the more differentiated multi-layers. Differences between the clinical P. gingivalis strains, however, became obvious only on multi-layers. These partially differentiated cells may also better represent the individual subject variations. Mono-layer cultures, which are simpler to obtain, seem to be less precise. The importance of cell differentiation on bacterial adhesion needs more attention.
Papaioannou
W
W
Faculty of Medicine, Department of Periodontology, Research Group for Microbial Adhesion, Catholic University Leuven, Leuven, Belgium. PerioWP@hol.gr
van Steenberghe
D
D
Cassiman
J-J
JJ
Dierickx
K
K
Quirynen
M
M
D000328:Adult; D000368:Aged; D001422:Bacterial Adhesion / Q000502:physiology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D002908:Chronic Disease; D004847:Epithelial Cells / Q000382:microbiology; D004848:Epithelium / Q000382:microbiology; D005260:Female; D006801:Humans; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D010514:Periodontal Pocket / Q000382:microbiology*; D010518:Periodontitis / Q000382:microbiology; D016966:Porphyromonas gingivalis / Q000502:physiology*