title: Axonal ephrin-As and odorant receptors: coordinate determination of the olfactory sensory map.
abstract: Olfactory sensory neurons expressing a given odorant receptor (OR) project with precision to specific glomeruli in the olfactory bulb, generating a topographic map. In this study, we demonstrate that neurons expressing different ORs express different levels of ephrin-A protein on their axons. Moreover, alterations in the level of ephrin-A alter the glomerular map. Deletion of the ephrin-A5 and ephrin-A3 genes posteriorizes the glomerular locations for neurons expressing either the P2 or SR1 receptor, whereas overexpression of ephrin-A5 in P2 neurons results in an anterior shift in their glomeruli. Thus the ephrin-As are differentially expressed in distinct subpopulations of neurons and are likely to participate, along with the ORs, as one of a complement of guidance receptors governing the targeting of like axons to precise locations in the olfactory bulb.
authors.affiliation: Howard Hughes Medical Institute and Center for Neurobiology and Behavior, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.
authors.forename: Tyler
authors.initials: T
authors.lastname: Cutforth
authors.forename: Laurie
authors.initials: L
authors.lastname: Moring
authors.forename: Monica
authors.initials: M
authors.lastname: Mendelsohn
authors.forename: Adriana
authors.initials: A
authors.lastname: Nemes
authors.forename: Nirao M
authors.initials: NM
authors.lastname: Shah
authors.forename: Michelle M
authors.initials: MM
authors.lastname: Kim
authors.forename: Jonas
authors.initials: J
authors.lastname: Frisén
authors.forename: Richard
authors.initials: R
authors.lastname: Axel
mesh_terms: D000818:Animals; D001369:Axons / Q000378:metabolism*; D036384:Ephrin-A3 / Q000235:genetics / Q000378:metabolism*; D036386:Ephrin-A5 / Q000235:genetics / Q000378:metabolism*; D017403:In Situ Hybridization; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009475:Neurons, Afferent / Q000166:cytology / Q000378:metabolism; D009830:Olfactory Bulb / Q000033:anatomy & histology / Q000502:physiology*; D051842:Olfactory Marker Protein; D018035:Receptors, Odorant / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012677:Sensation / Q000502:physiology*
chemical_list: D036384:Ephrin-A3; D036386:Ephrin-A5; D009419:Nerve Tissue Proteins; D051842:Olfactory Marker Protein; C497598:Omp protein, mouse; D018035:Receptors, Odorant; D011993:Recombinant Fusion Proteins