title: Down regulation of human immunodeficiency virus type-1 (HIV-1) expression by Vif antisense RNA expression vectors in transfected cells.
abstract: The HIV-1 vif gene is a potential candidate in the quest for anti-retroviral interventions, due to its unique role in the target cell infection. We employed the antisense RNA strategy to determine the antiviral activity of intracellularly expressed anti-sense RNAs of various lengths complementary to the targeted HIV-1 vif gene. Expression vectors mediating the delivery of the vif-ORF, 5'-Vif, M-vif, and 3'-vif antisense RNAs under the CMV promoter were constructed using pcDNA 3.1. The COS cells transfected with the antisense vectors showed a steady-state of antisense RNA expression levels. In contrast, those co-transfected with the Infectious molecular clone, pNL-E, exhibited a significant reduction in the steady-state antisense RNA levels, which correlated with a significant reduction in p24 antigen production. Thus, this expression method for these antisense RNAs provides a promising gene therapy strategy for HIV-1.
authors.affiliation: Department of Industrial Chemistry, Chiba Institute of Technology, Tsudanuma, Narashino, Chiba 275-0016, Japan.
authors.forename: Jacob Samson
authors.initials: JS
authors.lastname: Barnor
authors.forename: Naoko
authors.initials: N
authors.lastname: Miyano-Kurosaki
authors.forename: Kazuya
authors.initials: K
authors.lastname: Yamaguchi
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kobayashi
authors.forename: Koichi
authors.initials: K
authors.lastname: Ishikawa
authors.forename: Mubarak
authors.initials: M
authors.lastname: Osei-Kwasi
authors.forename: William Kwabina
authors.initials: WK
authors.lastname: Ampofo
authors.forename: David
authors.initials: D
authors.lastname: Ofori-Adjei
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Inagaki
authors.forename: Naoki
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takaku
mesh_terms: D000818:Animals; D019556:COS Cells; D003587:Cytomegalovirus / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics*; D016346:Gene Products, vif / Q000235:genetics*; D005822:Genetic Vectors; D015497:HIV-1 / Q000235:genetics*; D016366:Open Reading Frames; D011401:Promoter Regions, Genetic; D016372:RNA, Antisense / Q000235:genetics*; D014162:Transfection; D054320:vif Gene Products, Human Immunodeficiency Virus
chemical_list: D016346:Gene Products, vif; D016372:RNA, Antisense; D054320:vif Gene Products, Human Immunodeficiency Virus