Mass spectrometric examinations of stratum corneum lipid models exposed to ultraviolet irradiation.
Lipid model systems consisting of the major components of the stratum corneum intercellular lipid matrix were studied to investigate the ultraviolet-radiation-mediated damage of these biomolecules. Pure lipids and liposomes were irradiated using a lamp emitting a solar radiation spectrum. The influences of the irradiation and the effects of added iron ions were studied by electrospray ionization mass spectrometry (MS) with an ion trap analyser. Exact mass measurements were carried out using a time-of-flight mass spectrometer. Only linolenic acid and cholesterol were found to be subject to oxidative changes caused by UV irradiation whereas the other lipids examined (dipalmitoylphosphatidylcholine, ceramide III and cholesterol sulphate) were stable to oxidative stress. Several lipid adducts were observed upon analysis of the liposomes. The composition of these adducts was identified by MS/MS experiments.
Trommer
H
H
Institute of Pharmaceutics and Biopharmaceutics, School of Pharmacy, Martin Luther University Halle-Wittenberg, Germany.
Plätzer
M
M
Wolf
R
R
Neubert
R H H
RH
D002518:Ceramides / Q000737:chemistry / Q000528:radiation effects; D002784:Cholesterol / Q000737:chemistry / Q000528:radiation effects; D002788:Cholesterol Esters / Q000737:chemistry / Q000528:radiation effects; D004817:Epidermis / Q000737:chemistry*; D006801:Humans; D008055:Lipids / Q000737:chemistry / Q000528:radiation effects*; D008081:Liposomes; D008954:Models, Biological; D010084:Oxidation-Reduction; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D014466:Ultraviolet Rays / Q000009:adverse effects*; D017962:alpha-Linolenic Acid / Q000737:chemistry / Q000528:radiation effects
D002518:Ceramides; D002788:Cholesterol Esters; D008055:Lipids; D008081:Liposomes; D017962:alpha-Linolenic Acid; D002784:Cholesterol; C007045:cholesteryl sulfate