Nod factor inhibition of reactive oxygen efflux in a host legume.
Hydrogen peroxide (H(2)O(2)) efflux was measured from Medicago truncatula root segments exposed to purified Nod factor and to poly-GalUA (PGA) heptamers. Nod factor, at concentrations > 100 pM, reduced H(2)O(2) efflux rates to 60% of baseline levels beginning 20 to 30 min after exposure, whereas the PGA elicitor, at > 75 nM, caused a rapid increase in H(2)O(2) efflux to >200% of baseline rates. Pretreatment of plants with Nod factor alters the effect of PGA by limiting the maximum H(2)O(2) efflux rate to 125% of that observed for untreated plants. Two Nod factor-related compounds showed no ability to modulate peroxide efflux, and tomato (Lycopersicon esculentum), a nonlegume, showed no response to 1 nM Nod factor. Seven M. truncatula mutants, lacking the ability to make nodules, were tested for Nod factor effects on H(2)O(2) efflux. The nfp mutant was blocked for suppression of peroxide efflux, whereas the dmi1 and dmi2 mutants, previously shown to be blocked for early Nod factor responses, showed a wild-type peroxide efflux modulation. These data demonstrate that exposure to Nod factor suppresses the activity of the reactive oxygen-generating system used for plant defense responses.
Shaw
Sidney L
SL
Department of Biological Sciences, Stanford University, Stanford, California 94305-5020, USA. squid@cmgm.stanford.edu
Long
Sharon R
SR
D003470:Culture Media; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D029902:Medicago / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D019255:NADPH Oxidases / Q000037:antagonists & inhibitors / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D016897:Respiratory Burst; D013379:Substrate Specificity; D013997:Time Factors
D003470:Culture Media; D008070:Lipopolysaccharides; D006861:Hydrogen Peroxide; D019255:NADPH Oxidases