title: Protection from solar simulated radiation-induced DNA damage in cultured human fibroblasts by three commercially available sunscreens.
abstract: Exposure to solar radiation can produce both acute and chronic changes in the skin, including sunburn, edema, immunosuppression, premature skin aging, and skin cancer. At the cellular level, solar radiation can produce adverse structural and functional changes in membrane proteins and lipids and in chromosomal and mitochondrial DNA. The increasing awareness of these adverse effects has led the public to demand better photoprotection. In this study, the alkaline comet assay was used to evaluate the photoprotective effects of three commercially available sunscreens at sun protection factors (SPF) 15 and 30. Human fibroblasts were used as target cells to conveniently study the effects of solar simulated radiation on DNA damage in the presence and absence of sunscreens. When human fibroblasts were exposed to various doses of solar simulated radiation, DNA damage, as measured in sunscreen-protected cells by the comet assay, was not significantly different from that detected in unexposed cells. At 1.0 and 1.5 minimal erythemal doses (MED), all sunscreens, at both SPF 15 and 30, provided nearly 100% photoprotection to the fibroblasts. Further studies are required to elucidate the role of UVA in the production and repair of DNA damage in cells exposed to sunlight.
authors.affiliation: Consumer and Clinical Radiation Protection Bureau, Product Safety Programme, Health Canada, 775 Brookfield Road, Ottawa, ON K1A 1C1, Canada. Pascale_Reinhardt@hc-sc.gc.ca
authors.forename: Pascale
authors.initials: P
authors.lastname: Reinhardt
authors.forename: Michelle
authors.initials: M
authors.lastname: Cybulski
authors.forename: James P
authors.initials: JP
authors.lastname: McNamee
authors.forename: Jack R
authors.initials: JR
authors.lastname: McLean
authors.forename: Wayne
authors.initials: W
authors.lastname: Gorman
authors.forename: Yvon
authors.initials: Y
authors.lastname: Deslauriers
mesh_terms: D000704:Analysis of Variance; D002478:Cells, Cultured; D004249:DNA Damage; D005347:Fibroblasts / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects*; D006801:Humans; D015595:Skin Aging / Q000187:drug effects / Q000502:physiology; D013473:Sunscreening Agents / Q000494:pharmacology*; D014466:Ultraviolet Rays / Q000009:adverse effects
chemical_list: D013473:Sunscreening Agents