[Effect of Angelica sinensis polysaccharides on lymphocyte proliferation and induction of IFN-gamma].
OBJECTIVE
To study the effect of Angelica sinsensis polysaccharides on lymphocyte proliferation and induction of IFN-gamma.


METHODS
Angelica sinensis polysaccharides(AP) were separated into AP-I, AP-II, AP-III and AP-IV by alcohol deposition with different concentration. The radioactivities of [3H]-TdR uptake by lymphocyte were used to determine the ability of lymphocyte. The bioactivity of IFN-gamma was measured by violet crystalline dying.


RESULTS
AP-IV was found to be composed of Ara and Glu in the ratio of 0.99:6.47, the molecular weight was estimated to be 5,600. AP-I and AP-II 100 mg.kg-1 i.p. were found to significantly augment mice splenocyte proliferation, release IFN-gamma and increase IFN-gamma bioactivity. 50 micrograms.mL-1 AP-I, AP-II and AP-III were shown to enhance the proliferative response of the mouse spleen lymphocytes in vitro.


CONCLUSIONS
AP-I and AP-II showed higher immunoactivity than AP-III, AP-IV had no effect.
Shan
Jun-jie
JJ
Institute of Chinese Materia Medica, Shanghai Academy of Traditional Chinese Medicine, Shanghai 200032, China. shanjunjie001@sohu.com
Wang
Yi
Y
Wang
Shun-chun
SC
Liu
Di
D
Hu
Zhi-bi
ZB
D029971:Angelica sinensis / Q000737:chemistry*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D007109:Immunity / Q000187:drug effects; D007371:Interferon-gamma / Q000096:biosynthesis*; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010946:Plants, Medicinal / Q000737:chemistry; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D011897:Random Allocation; D013154:Spleen / Q000166:cytology; D014407:Tumor Cells, Cultured
D004365:Drugs, Chinese Herbal; D011134:Polysaccharides; D007371:Interferon-gamma