Novel poly(ethylene glycol) derivatives with carboxylic acid pendant groups: synthesis and their protection and enhancing effect on non-viral gene transfection systems.
Novel carboxylic acid pendant-containing poly(ethylene glycol) (PEG) derivatives (PEG-Cs) were synthesized by the chemical modification of the carbon-carbon double-bond side chain of copoly(allyl glycidyl ether/ethylene oxide) (copoly(AGE/EO)). PEG-C showed a protecting ability for the DNA/polycation complex from the albumin-induced aggregation. It also expressed the enhanced efficiency on the polycation-mediated gene transfection on the cultured cells (up to 3-4-times higher), probably due to its disperse-stabilizing property and also the proton-sponge effect.
Koyama
Yoshiyuki
Y
Department of Home Economics, Otsuma Women's University, Chiyoda-ku, Tokyo 102-8357, Japan. koyama@otsuma.ac.jp
Ito
Tomoko
T
Matsumoto
Hidetoshi
H
Tanioka
Akihiko
A
Okuda
Tatsuya
T
Yamaura
Nobuaki
N
Aoyagi
Haruhiko
H
Niidome
Takuro
T
D000818:Animals; D016466:CHO Cells; D019556:COS Cells; D002264:Carboxylic Acids / Q000737:chemistry*; D002412:Cations / Q000737:chemistry / Q000378:metabolism; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000378:metabolism; D004987:Ethers / Q000737:chemistry; D005027:Ethylene Oxide / Q000737:chemistry; D010084:Oxidation-Reduction; D011092:Polyethylene Glycols / Q000138:chemical synthesis* / Q000737:chemistry*; D011196:Potassium Permanganate / Q000737:chemistry; D013499:Surface Properties; D014162:Transfection
D002264:Carboxylic Acids; D002412:Cations; D004987:Ethers; D011196:Potassium Permanganate; D011092:Polyethylene Glycols; D004247:DNA; D005027:Ethylene Oxide