title: A peptide antibody for rapid screening of Streptomyces species producing phospholipase D.
abstract: By examining the conserved regions in the protein sequences of eight different Streptomyces phospholipase Ds (PLD) reported so far and the X-ray crystallographic structure of a Streptomyces PLD, we designed a peptide sequence, DPANRGAVGSGGYSQIKSL, for the screening of microorganisms producing PLD. In the enzyme-linked immunosorbent assay using a mouse antibody raised against the designed peptide, we recovered seven producing strains out of 128 soil isolates.
authors.affiliation: Faculty of Biological Sciences and Institute for Molecular Biology and Genetics, Chonbuk National University, Jeonju 561-756, Republic of Korea. tbuhm@moak.chonbuk.ac.kr
authors.forename: Tai-Boong
authors.initials: TB
authors.lastname: Uhm
authors.forename: Sun Hee
authors.initials: SH
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents; D017124:Conserved Sequence; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004789:Enzyme Activation; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D008969:Molecular Sequence Data; D010455:Peptides; D010739:Phospholipase D / Q000032:analysis* / Q000096:biosynthesis*; D015202:Protein Engineering / Q000379:methods; D020539:Sequence Analysis, Protein / Q000379:methods; D013045:Species Specificity; D013302:Streptomyces / Q000145:classification / Q000201:enzymology* / Q000302:isolation & purification*
chemical_list: D000900:Anti-Bacterial Agents; D010455:Peptides; D010739:Phospholipase D