title: A practical high-throughput screening system for enantioselectivity by using FTIR spectroscopy.
abstract: For the first time FTIR spectroscopy has been applied to the measurement of enantiomeric purity. The underlying concept is based on the use of pseudo-enantiomers that are (13)C-labeled at appropriate positions. Upon applying Lambert-Beer's law in the determination of the concentrations of both enantiomers, the ee values are accessible, accuracy to within +/-5 % of the true values being possible. The application of a commercially available high-throughput FTIR system results in a slightly decreased accuracy (+/-7% for the ee values), but this allows a throughput of up to 10000 samples per day. The method is of interest in the area of combinatorial symmetric catalysis and directed evolution of enantioselective enzymes.
authors.affiliation: Max-Planck-Institut für Kohlenforschung Kaiser-Wilhelm-Platz 1, 45470 Mülheim/Ruhr, Germany.
authors.forename: Patrick
authors.initials: P
authors.lastname: Tielmann
authors.forename: Matthias
authors.initials: M
authors.lastname: Boese
authors.forename: Martin
authors.initials: M
authors.lastname: Luft
authors.forename: Manfred T
authors.initials: MT
authors.lastname: Reetz