Stereospecific biotransformation of albendazole in mouflon and rat-isolated hepatocytes.
The anthelmintic albendazole (ABZ) undergoes a two-step oxidation resulting first in the formation of chiral albendazole sulfoxide (ABZSO) followed by its transformation to albendazole sulfone (ABZSO2) in many farm and laboratory animal species. Although cloven-hoofed game are also treated with ABZ, limited information concerning ABZ biotransformation in these species is available. The present study focused on in vitro ABZ sulfoxidation in hepatocytes from wild sheep-mouflon (Ovis musimon) and comparison of ABZ sulfoxidation in mouflon and rat (Rattus norvergicus) hepatocytes. ABZ was used as a substrate for primary cultures of mouflon and rat hepatocytes. Time-dependent stereospecific consumption of ABZSO and ABZSO2 formation has been investigated. The metabolites were determined by high-performance liquid chromatography with both achiral and chiral stationary phases. Although total-ABZSO formation did not significantly differ between mouflon and rat, after separation of the (+)-ABZSO and (-)-ABZSO enantiomers a significant difference between species was found. The enantiomeric ratio of (+)/(-)-ABZSO in mouflon hepatocytes was 2.8-3.8, while rat hepatocytes biotransformed ABZ to almost racemic ABZSO, with an enantiomeric ratio of 1.0-1.1. The ratio were similar for two concentrations of substrate used and stable over several time intervals. The formation of ABZSO2 was more extensive in rat (approximately five times) than in mouflon hepatocytes.
Velík
J
J
Department of Pharmacology and Toxicology and Department of Biochemical Sciences, Faculty of Pharmacy, Charles University, Hradec Králové, Czech Republic. 95vej2299@faf.cuni.cz
Baliharová
V
V
Skálová
L
L
Szotáková
B
B
Wsól
V
V
Lamka
J
J
D015766:Albendazole / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D000835:Animals, Wild; D000871:Anthelmintics / Q000737:chemistry / Q000378:metabolism*; D001711:Biotransformation; D018929:Cell Culture Techniques; D002851:Chromatography, High Pressure Liquid; D022781:Hepatocytes / Q000737:chemistry; D008297:Male; D051381:Rats; D012756:Sheep / Q000502:physiology*; D034561:Sheep, Domestic; D013237:Stereoisomerism
D000871:Anthelmintics; D015766:Albendazole