[Serial analysis of gene expression in the pituitary adenomas and para-tumor normal pituitary tissues].
OBJECTIVE
To observe the characteristics and difference of gene expression in the pituitary adenomas and para-tumor normal pituitary tissues.


METHODS
Using serial analysis of gene expression (SAGE), two SAGE libraries were generated. Forty clones from each SAGE library were sequenced, and the results were analyzed by SAGE2000 software and compared with the SAGE map at NCBI.


RESULTS
A total of 655 gene tags, representing 43 genes, were extracted from the 40 sequence files of the para-tumor normal pituitary tissues and 737 gene tags, representing 53 genes, were extracted from the 40 sequence files of the pituitary adenomas. Of these tags, 13 were not reported before. The genes related to pituitary hormone secretion and energy metabolism were highly expressed in the two kinds of tissues. Some growth factors and cytokines were also expressed, including those involved in the immunological system. But there were also much difference of gene expression in the two tissues. Thirty-one and five tags were only detected in para-tumor normal pituitary tissues and pituitary adenomas, respectively.


CONCLUSIONS
Genes involved in hormones secretion and energy metabolism were highly expressed in the pituitary adenomas and para-tumor normal pituitary tissues. Many growth factors and cytokines were also expressed in pituitary. There was also much difference of gene expression in the two kinds of tissues. SAGE can be used not only in understanding the quantity information of gene expression, but also in finding new genes.
Hu
Yao-fei
YF
Department of Neurosurgery, PUMC Hospital, CAMS, PUMC, Beijing 100730, China.
Ren
Zu-yuan
ZY
Li
Yun-feng
YF
Sun
Hong-xia
HX
Chang
Yong-sheng
YS
Su
Chang-bao
CB
Wang
Ren-zhi
RZ
Zuo
Jin
J
Fang
Fu-de
FD
D000236:Adenoma / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D020224:Expressed Sequence Tags; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D010902:Pituitary Gland / Q000378:metabolism*; D010911:Pituitary Neoplasms / Q000235:genetics* / Q000378:metabolism