Effects of diet formulations containing proteins from different sources on intestinal colonization by Campylobacter jejuni in broiler chickens.
The objective of this study was to compare the effects of 3 diet formulations containing different protein sources (animal, plant, and a combination of animal and plant) on the colonization of Campylobacter jejuni in the gastrointestinal tract of broiler chickens. A freshly isolated strain of C. jejuni (biotype IV, serotype HS O:21, O:29, HL untypable) from a broiler chicken was used to infect 3-day-old chicks that had been free of C. jejuni; 0.5 mL of an inoculum containing 10(8) colony-forming units was administered orally. Shedding of the organism was studied, and C. jejuni in the ceca, jejuni, and crop were enumerated by quantitative culture. The isolates recovered from the birds during the study period of 35 d were characterized and confirmed as C. jejuni by the use of standard methods and underwent biotyping, serotyping, antimicrobial susceptibility testing by disk diffusion and the E-test, and flagellin gene typing. A cyclical pattern of shedding of C. jejuni was observed in all the birds. Colonization was highest in the ceca. The ceca of birds receiving plant-protein-based feed had significantly less colonization then the ceca of birds receiving the other types of feed, whereas the differences in colonization of the jejuni and crops were not significant. Characterization by biotyping, serotyping, and flagellin gene typing showed that 95% of the recovered isolates were identical to the strain used for infecting the chicks. However, with the Lior-HL typing scheme, 74% of the recovered isolates were HL untypable. Antimicrobial resistance testing did not reveal significant differences between the infecting strain and the recovered isolates among the different feed groups.
Udayamputhoor
Roy S
RS
Departments of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Prince Edward Island C1A 4P3.
Hariharan
Harry
H
Van Lunen
Ted A
TA
Lewis
P Jeffrey
PJ
Heaney
Susan
S
Price
Lawrence
L
Woodward
David
D
D000284:Administration, Oral; D000818:Animals; D015373:Bacterial Typing Techniques / Q000662:veterinary; D002169:Campylobacter Infections / Q000382:microbiology / Q000662:veterinary*; D016123:Campylobacter jejuni / Q000187:drug effects / Q000472:pathogenicity / Q000502:physiology*; D002432:Cecum / Q000382:microbiology; D002645:Chickens; D015169:Colony Count, Microbial / Q000662:veterinary; D004044:Dietary Proteins / Q000008:administration & dosage* / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D007422:Intestines / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D010940:Plant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D011201:Poultry Diseases / Q000382:microbiology*; D011897:Random Allocation
D004044:Dietary Proteins; D010940:Plant Proteins