Accessibility of cysteine residues in a cytoplasmic loop of CitS of Klebsiella pneumoniae is controlled by the catalytic state of the transporter.
The citrate transporter CitS of Klebsiella pneumoniae is a secondary transporter that transports citrate in symport with two sodium ions and one proton. Treatment of CitS with the alkylating agent N-ethylmaleimide resulted in a complete loss of transport activity. Treatment of mutant proteins in which the five endogenous cysteine residues were mutated into serines in different combinations revealed that two cysteine residues located in the C-terminal cytoplasmic loop, Cys-398 and Cys-414, were responsible for the inactivation. Labeling with the membrane impermeable methanethiosulfonate derivatives MTSET and MTSES in right-side-out membrane vesicles showed that the cytoplasmic loop was accessible from the periplasmic side of the membrane. The membrane impermeable but more bulky maleimide AmdiS did not inactivate the transporter in right-side-out membrane vesicles. Inactivation by N-ethylmaleimide, MTSES, and MTSET was prevented by the presence of the co-ion Na(+). Protection was obtained upon binding 2 Na(+), which equals the transport stoichiometry. In the absence of Na(+), the substrate citrate had no effect on the inactivation by permeable or impermeable thiol reagents. In contrast, when subsaturating concentrations of Na(+) were present, citrate significantly reduced inactivation suggesting ordered binding of the substrate and co-ion; citrate is bound after Na(+). In the presence of the proton motive force, the reactivity of the Cys residues was increased significantly for the membrane permeable N-ethylmaleimide, while no difference was observed for the membrane impermeable thiol reagents. The results are discussed in the context of a model for the opening and closing of the translocation pore during turnover of the transporter.
Sobczak
Iwona
I
Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Haren, The Netherlands.
Lolkema
Juke S
JS
D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D002247:Carbon Isotopes; D002352:Carrier Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D002384:Catalysis; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D002463:Cell Membrane Permeability; D019343:Citric Acid / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology; D003545:Cysteine / Q000235:genetics / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D005033:Ethylmaleimide / Q000494:pharmacology; D007711:Klebsiella pneumoniae / Q000737:chemistry*; D008301:Maleimides / Q000737:chemistry / Q000494:pharmacology; D008698:Mesylates / Q000737:chemistry / Q000494:pharmacology; D018892:Proton-Motive Force / Q000502:physiology; D012964:Sodium / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D022161:Transport Vesicles / Q000737:chemistry / Q000378:metabolism
D001426:Bacterial Proteins; D002247:Carbon Isotopes; D002352:Carrier Proteins; C086246:CitS protein, bacteria; D008301:Maleimides; D008698:Mesylates; C043592:maleimide; D019343:Citric Acid; C074502:methanethiosulfonate; D012964:Sodium; D003545:Cysteine; D005033:Ethylmaleimide