Kinetic study of derivatives of phenylcarbamic acid enantiomers in rabbit blood serum using an on-line coupled column liquid chromatographic system.
An on-line coupled HPLC system is described for the kinetic study of the enantiomers of 1-methyl-2-piperidinoethylesters of 2-, 3- and 4-alkoxyphenylcarbamic acid in rabbit blood serum. The method involves three steps: (i) off-line pre-concentration and cleanup step, (ii) analytical separation of the racemate on a reversed-phase stationary phase, and (iii) separation of the enantiomers on a teicoplanin aglycone chiral stationary phase (Chirobiotic Tag). The limit of the determination with standard solutions was approximately 5.0 microg/ml. In vitro degradation studies of enantiomers have demonstrated differences in the concentration of the enantiomers after the treatment. The rate constants of R(-)- and S(+)-forms of enantiomers (the same as the position of alkoxychain) were, not significantly different. The number of carbon atoms had an influence on the degradation kinetics.
Rojkovicová
T
T
Department of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology, Bratislava, Slovak Republik.
Lehotay
J
J
Cizmárik
J
J
D000818:Animals; D002219:Carbamates / Q000097:blood / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid; D009862:Online Systems; D011817:Rabbits; D013237:Stereoisomerism; D017334:Teicoplanin / Q000737:chemistry
D002219:Carbamates; D017334:Teicoplanin; C059692:phenylcarbamic acid