The use of Fourier-transform infrared spectroscopy for the quantitative determination of glucose concentration in whole blood.
Fourier-transform infrared transmission spectroscopy has been used for the determination of glucose concentration in whole blood samples from 28 patients. A 4-vector partial least-squares calibration model, using the spectral range 950-1200 cm(-1), yielded a standard-error-of-prediction of 0.59 mM for an independent test set. For blood samples from a single patient, we found that the glucose concentration was proportional to the difference between the values of the second derivative spectrum at 1082 cm(-1) and 1093 cm(-1). This indicates that spectroscopy at these two specific wavenumbers alone could be used to determine the glucose concentration in blood plasma samples from a single patient, with a prediction error of 0.95 mM.
Shen
Y C
YC
Cavendish Laboratory, University of Cambridge, Madingley Road, Cambridge CB3 0HE, UK.
Davies
A G
AG
Linfleld
E H
EH
Elsey
T S
TS
Taday
P F
PF
Arnone
D D
DD
D001786:Blood Glucose / Q000032:analysis*; D002138:Calibration; D006801:Humans; D015233:Models, Statistical; D015999:Multivariate Analysis; D015203:Reproducibility of Results; D013053:Spectrophotometry / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D001786:Blood Glucose