title: Identification of a bisegmented double-stranded RNA virus (picobirnavirus) in calf faeces.
abstract: To determine the incidence of rotavirus infection among dairy herds in the State of São Paulo, Brazil, 576 faecal samples obtained from calves aged 1-45 days with and without diarrhoea, reared on 63 dairy cattle farms, were analyzed. Polyacrylamide gel electrophoresis (PAGE) identified 28 samples positive for group A rotavirus, while four samples, two diarrhoeic and two non-diarrhoeic, showed a bisegmented genome with a typical picobirnavirus pattern. Electron microscopy revealed spherical virus particles with a diameter of 37 nm and without a defined surface structure. The present study is the first report of a bisegmented virus identified in cattle in Brazil.
authors.affiliation: Departamento de Medicina Veterinária Preventiva e Reprodução Animal, Universidade Estadual Paulista, Via de Acesso Prof. Paulo Donato Castellane, s/n, 14884-900 Jaboticabal, Estado de São Paulo, Brazil. gloriinha@fcav.unesp.br
authors.forename: M G
authors.initials: MG
authors.lastname: Buzinaro
authors.forename: P P S
authors.initials: PP
authors.lastname: Freitas
authors.forename: J J
authors.initials: JJ
authors.lastname: Kisiellius
authors.forename: M
authors.initials: M
authors.lastname: Ueda
authors.forename: J A
authors.initials: JA
authors.lastname: Jerez
mesh_terms: D000818:Animals; D001938:Brazil; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D003967:Diarrhea / Q000662:veterinary / Q000821:virology; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D005243:Feces / Q000821:virology; D008854:Microscopy, Electron; D018708:Picobirnavirus / Q000302:isolation & purification*; D012330:RNA, Double-Stranded / Q000302:isolation & purification*
chemical_list: D012330:RNA, Double-Stranded