[Research on expansion of CD34+ cells from umbilical cord blood using different cytokine groups in vitro].
OBJECTIVE
To investigate the expansion of the CD34+ cells of umbilical cord blood in vitro culture comprising different cytokines and the potential hematopoiesis function of the expanded CD34+ cells.


METHODS
Expanding the purified CD34+ cells from the fresh umbilical cord blood for 6 days in vitro with the use of different combination of FL, SCF, TPO, IL-3 and IL-6. Getting one better combination by analyses of the results from flowcytometry, LTC-IC, and progenitor clonogenic assays.


RESULTS
The numbers of CD34+ cells expanded by use of different combination of cytokines were 3.1-8.6 times as large as the numbers of ordinary ones, thus making 16 cases of insufficient CD34+ cells become able to satisfy the needs of the adults weighing 50 kg. No significant difference was observed between the function of the expanded cells and the original ones in hematopoiesis. Meanwhile, it was proved that TPO in combination with other cytokines could strengthen the expansion of CD34+ cells, but using TPO alone for long time culture would cause more CD34+ cells to be megalocaryocytes.


CONCLUSIONS
The combination of FST36 cytokines can expand the CD34+ cells of the umbilical cord blood to meet the adults' needs in laboratory's methods.
Tang
Se
S
Department of Pediatrics, West China Second Hospital Sichuan University, Chengdu 610041, China.
Li
Yunxing
Y
Wang
Meijing
M
Yang
Chongli
C
D018952:Antigens, CD34 / Q000097:blood*; D002454:Cell Differentiation / Q000187:drug effects / Q000276:immunology; D002455:Cell Division / Q000187:drug effects / Q000276:immunology; D002469:Cell Separation; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D016207:Cytokines / Q000494:pharmacology*; D005312:Fetal Blood / Q000166:cytology*; D006410:Hematopoiesis / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007377:Interleukin-3 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology
D018952:Antigens, CD34; D016895:Culture Media, Serum-Free; D016207:Cytokines; D007377:Interleukin-3; D015850:Interleukin-6