title: Molecular cloning and cDNA sequence analysis of carboxypeptidases A1, A2 and B from the Japanese flounder Paralichthys olivaceus.
abstract: Although pancreatic serine proteases have been cloned in teleosts, no sequence data are currently available on members of the carboxypeptidase (CP) family. Here, we cloned cDNAs coding for two preproCPAs, corresponding to mammalian preproCPA1 and preproCPA2, and one preproCPB from a pancreatic cDNA library of the Japanese flounder, Paralichthys olivaceus. The activation peptides of flounder proCPs completely retained the sequences for inhibition of enzymatic activity of proCPs just like mammalian proCPs. Of 306-309 amino acids in total, 95 amino acids are completely conserved between bovine CPA1 and CPB and flounder CPs. Notably, amino acid residues for Zn(2+) ligands, catalysis and substrate anchoring are completely conserved between flounder and bovine CPs. Three species of flounder preproCPs are all expressed in the pancreas of first feeding larvae.
authors.affiliation: National Research Institute of Aquaculture, Nansei, Mie 516-0193, Japan.
authors.forename: Anand S
authors.initials: AS
authors.lastname: Srivastava
authors.forename: Tadahide
authors.initials: T
authors.lastname: Kurokawa
authors.forename: Tohru
authors.initials: T
authors.lastname: Suzuki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D043424:Carboxypeptidase B / Q000145:classification / Q000235:genetics*; D043422:Carboxypeptidases A / Q000145:classification / Q000235:genetics*; D002417:Cattle; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis*; D005260:Female; D005432:Flounder / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D006801:Humans; D017403:In Situ Hybridization; D007814:Larva / Q000033:anatomy & histology / Q000502:physiology; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; D010455:Peptides; D043422:Carboxypeptidases A; D043424:Carboxypeptidase B