title: [A-Factor as a selective agent for isolation of the soil Gram-negative bacterium strain--the producent of an antibacterial antibiotic].
abstract: It was shown the stimulating role of A-factor on soil prokaryotes growth. Soil sample culturing on agar medium, containing A-factor, resulted in the colony forming units (CFU) increasing in comparison with culturing on the medium without this regulator. Gram-negative bacteria were the reason of CFU increasing; previously the effect of A-factor on bacteria of this group was not shown. Gram-negative rod bacterial strain No. 35 was isolated and shown that CFU number was approximately twice increased at A-factor concentration in agar medium 2 and 7 mcg/ml; high A-factor concentration up to 28 mcg/ml was not effective. Isolated strain No. 35 is the producer of antibiotic, effective against gram-positive bacteria.
authors.affiliation: G. F. Gause Institute of New Antibiotics, Russian Academy of Medical Sciences, Moscow.
authors.forename: V D
authors.initials: VD
authors.lastname: Gruzina
authors.forename: O V
authors.initials: OV
authors.lastname: Efremenkova
authors.forename: V A
authors.initials: VA
authors.lastname: Zenkova
authors.forename: M I
authors.initials: MI
authors.lastname: Reznikova
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Dudnik
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000378:metabolism* / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D015169:Colony Count, Microbial; D003470:Culture Media; D017753:Ecosystem; D006090:Gram-Negative Bacteria / Q000302:isolation & purification* / Q000378:metabolism; D006094:Gram-Positive Bacteria / Q000254:growth & development; D006133:Growth Substances / Q000378:metabolism* / Q000494:pharmacology; D012988:Soil Microbiology
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D006133:Growth Substances; C025035:A-factor (Streptomyces); D015107:4-Butyrolactone