[Gene therapy of rat prolactinomas mediated by adenoviral vectors with rat tyrosine hydroxylase gene].
OBJECTIVE
To investigate the potential of gene therapy of rat prolactinomas mediated by adenoviral vectors with a gene encoding rat tyrosine hydroxylase.


METHODS
Recombinant replication-deficient adenovirus named Ad-GFP-TH with rat TH-cDNA and control adenovirus named Ad-GFP were constructed by homologous recombination in bacterial cells. The rat pituitary prolactinoma cell line MMQ are chosen as the target cells to study the effect of gene therapy on their growth and prolactin secretion mediated by Ad-GFP-TH.


RESULTS
Recombinant Ad-GFP-TH and Ad-GFP were successfully reconstructed. Transfection of MMQ cells with Ad-GFP-TH not only restrained their growth but also decreased their PRL secretion.


CONCLUSIONS
Gene therapy may serve for a potential treatment for prolactinomas, especially invasive prolactinomas.
Xu
Zhi-qin
ZQ
Department of Neurosurgery, PUMC Hospital, CAMS, PUMC, Beijing 100730, China. xuzhq@csc.pumch.ac.cn
Su
Chang-bao
CB
Chen
Song-sen
SS
Ren
Zu-yuan
ZY
Di
Xu
X
Ma
Wen-bin
WB
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D015316:Genetic Therapy; D005822:Genetic Vectors; D010911:Pituitary Neoplasms / Q000628:therapy*; D015175:Prolactinoma / Q000628:therapy*; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection; D014446:Tyrosine 3-Monooxygenase / Q000096:biosynthesis / Q000235:genetics*
D011994:Recombinant Proteins; D014446:Tyrosine 3-Monooxygenase