title: Selective formation of non-natural type triplexes containing TA interrupting sites with the TFO incorporating W-shape nucleic acid (WNA) analogs.
abstract: Novel nucleoside analogs have been desired for selective formation of non-natural type triplexes containing TA or CG interrupting sites. We have previously reported that the W-shaped nucleic acid (WNA) would be a useful skeleton to develop new base analog for the formation of non-natural triplexes of antiparallel motif. In this study, we have found that triplex forming oligonucleotide (TFO) incorporating the new WNA analog, WNA-beta T, formed more stable triplex than natural triplex with high selectivity to the TA site. It is also noted that the TFO containing WNA-beta T did not form aggregates at the physiological condition of K+.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Taniguchi
authors.forename: Ryo
authors.initials: R
authors.lastname: Takahashi
authors.forename: Keiichi
authors.initials: K
authors.lastname: Kodama
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Senko
authors.forename: Minoru
authors.initials: M
authors.lastname: Maeda
authors.forename: Shigeki
authors.initials: S
authors.lastname: Sasaki
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D009696:Nucleic Acids / Q000737:chemistry*
chemical_list: D009696:Nucleic Acids