[Specific determination of flaviviruses by molecular hybridization with synthetic deoxyoligonucleotide probes].
Molecular probes were designed for the purpose of specific determination of flavioviruses transmitted by the ticks of tick-borne encephalitis (TBE) and Omsk hemorrhagic fever (OHV) as well as by mosquitoes of Japanese encephalitis (JE), North Nile (NN), Murrey Valley encephalitis (MVE), Saint-Lois encephalitis (SLE), dengue 1-4 and of yellow fever (YF). The probes are synthetic deoxyoligonucleotides with the 18-20 long basis and complementary for the RNA fragments defined by computer analysis. The thus obtained probes, which specifically hybridize themselves with the sets of the TBE virus or of the OHV virus and do not hybridize themselves with other TBE viruses' sets. Group-specific probes for YE and dengue viruses as well as virus-specific probes, which are able to detect each of the above viruses without any cross effects, were suggested for indexing and identifying the flaviviruses transmitted by mosquitoes.
Zlobin
V I
VI
Pogodina
V V
VV
Drokin
D A
DA
Gazo
M Kh
MKh
Bochkova
N G
NG
Dzhioev
Iu P
IuP
Demina
T V
TV
Belikov
S I
SI
Kozlova
I V
IV
Verkhozina
M M
MM
D004669:Encephalitis Viruses, Tick-Borne / Q000235:genetics / Q000302:isolation & purification; D005416:Flavivirus / Q000235:genetics* / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000138:chemical synthesis / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014759:Viral Envelope Proteins / Q000235:genetics
D015345:Oligonucleotide Probes; D012367:RNA, Viral; D014759:Viral Envelope Proteins; C065696:glycoprotein E, Flavivirus