[Morphologic observation of papaya on anoxic damaged cerebral neurocytes of therapeutic efficacy in vitro].
OBJECTIVE
To observe the effects of papaya on the therapeutic efficacy of cerebral neurocytes following anoxic damage in vitro.


METHODS
The rat embryonic cerebral neurocytes were cultured with serum-free medium. Papapa was added in while anoxic damage occurred and the neurocytes were divided into four groups (group I-anoxic damage 0 mg/ml, group II-anoxic damage 0.1 mg/ml, group III-anoxic damage 0.5 mg/ml and group-IV non-anoxic damage 0 mg/ml). At the end of the experiment, cultured neurocytes were collected and biochemical indexes were detected and, a pathological observation was made by using electronic microscope.


RESULTS
The activity of MTT in group II was significantly higher than that in the other three groups(P < 0.01). Compared with group I and IV, the level of TCHE in group II and III was increased significantly(P < 0.05), and the level of TCHE in group III was much higher than that in group II (P < 0.05). The morphological observation showed that the number of neurocytes in group I decreased, and the appearance of cell was broken, the outer limits were not clear, apophysises were thin and short, and apoptosis body can be found. In group II and III, stretched preparation of majority cells was good, the bodies of neurocytes was full-grown, the outer limits were clear, apophysises were strong and network was dense, chromatin was partly condense, but apoptosis bodies can not be found.


CONCLUSIONS
Papaya can promote the recovery of anoxic damage of rat cerebral neurocytes in structure.
Chang
Hong
H
Nutrition Research Unit, Tianjin Medical University, Tianjin 300070, China.
Huang
Guowei
G
Dong
Yali
Y
Zhang
Wenzhi
W
Su
Xin
X
Liu
Li
L
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000166:cytology* / Q000196:embryology; D029441:Carica; D015687:Cell Hypoxia; D002478:Cells, Cultured; D005260:Female; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D028321:Plant Preparations / Q000494:pharmacology*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar
D028321:Plant Preparations; D000110:Acetylcholinesterase