title: [Construction of a collagen gel dermis with improved biological property].
abstract: OBJECTIVE
  To improve the biological property of artificial skin.
  
  
  METHODS
  We have ameliorated Hansburgh and Middelkoop's method of manufacturing artificial dermis. The type I collagenase and Dispase were used to isolated neonate prepuce' dermis fibroblast. The gel dermis was constructed by compounding the fibroblast and collagen swelling solution. The property of the collagen gel dermis was measured.
  
  
  RESULTS
  The neonate prepuce's dermis fibroblast had property of high proliferation, high activation of the dermis, and it could secrete abundant extracellular matrix (ECM).
  
  
  CONCLUSIONS
  The collagen gel dermis is an useful dermis substitute.
authors.affiliation: Department of Biological Material, Institute of Biomedical Engineering, CAMS, PUMC, Tianjin 300192, China.
authors.forename: Ling-rong
authors.initials: LR
authors.lastname: Liu
authors.forename: Li-hai
authors.initials: LH
authors.lastname: Zhang
authors.forename: Dong-rui
authors.initials: DR
authors.lastname: Ma
authors.forename: Qi-qing
authors.initials: QQ
authors.lastname: Zhang
mesh_terms: D002469:Cell Separation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis; D020405:Dermis / Q000166:cytology / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D017768:Skin, Artificial; D023822:Tissue Engineering
chemical_list: D024042:Collagen Type I; D016326:Extracellular Matrix Proteins