[Stage-dependent expression of androgen binding protein mRNA in sertoli cell of rat testis].
OBJECTIVE
To investigate the variation of rat androgen binding protein (ABP) mRNA in sertoli cell of rat testis during spermatogenesis.


METHODS
In situ hybridization was performed with digoxin-labeled rat ABP cDNA probe on frozen testis tissue sections from SD rats. The expression of ABP mRNA was quantified by using laser density scanning system.


RESULTS
It was shown that ABP mRNA levels were low from stage I to stage VI, increased to the peak level at the stages VII-VIII, and then declined rapidly at stages IX-XI. Thereafter, ABP mRNA levels increased again at stages XII-XIV, and then decreased to the lowest level at stages I-VI.


CONCLUSIONS
ABP mRNA level was stage-dependent during the spermatogenic cycle.
Chen
Y
Y
Department of Histology and Embryology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China.
Ye
S
S
Huang
Y
Y
Zheng
W
W
D000727:Androgen-Binding Protein / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D017403:In Situ Hybridization; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012670:Seminiferous Epithelium / Q000502:physiology*; D012708:Sertoli Cells / Q000378:metabolism*; D013091:Spermatogenesis / Q000502:physiology*; D013737:Testis / Q000166:cytology
D000727:Androgen-Binding Protein; D012333:RNA, Messenger