Glutathione transport in the endo/sarcoplasmic reticulum.
Glutathione transport through the endo/sarcoplasmic reticulum (ER/SR) membrane might play a role in the maintenance of the thiol redox potential difference between the lumen and the cytosol. The transport of glutathione (both GSH and glutathione disulfide, GSSG) is entirely different in the ER and SR membranes. The transport measurements based on either rapid filtration or light scattering techniques revealed that the SR membrane transports glutathione much faster than the hepatic ER membrane or microsomal membranes prepared from heart or brain. The fastest transport has been measured in the membrane of muscle terminal cisternae, which is enriched in ryanodine receptor type 1 (RyR1). All the studied membranes have been found to be equally impermeable to various hydrophilic substances of similar size to glutathione, thus the glutathione transport in muscle microsomes and terminal cysternae as well as the correlation between the rate of glutathione transport and the abundance of RyR1 are specific. In both muscle microsomes and terminal cysternae, glutathione influx can be either inhibited or activated by antagonists and agonists of the ryanodine receptor, respectively, while these agents do not influence the transport of other small permeant molecules. These findings strongly suggest that the ryanodine receptor channel activity is directly associated with glutathione transport activity in the skeletal muscle sarcoplasmic reticulum membrane.
Csala
Miklós
M
Department of Medical Chemistry, Pathobiochemistry and Molecular Biology, Semmelweis University, H-1444, Budapest, POB. 260, Hungary.
Fulceri
Rosella
R
Mandl
József
J
Benedetti
Angelo
A
Bánhegyi
Gábor
G
D000818:Animals; D001692:Biological Transport; D001921:Brain / Q000648:ultrastructure; D003600:Cytosol / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism* / Q000648:ultrastructure; D005978:Glutathione / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism; D008099:Liver / Q000648:ultrastructure; D008861:Microsomes / Q000378:metabolism / Q000648:ultrastructure; D018482:Muscle, Skeletal / Q000648:ultrastructure; D009206:Myocardium / Q000648:ultrastructure; D010084:Oxidation-Reduction; D011817:Rabbits; D019837:Ryanodine Receptor Calcium Release Channel / Q000032:analysis; D012519:Sarcoplasmic Reticulum / Q000378:metabolism* / Q000648:ultrastructure; D013438:Sulfhydryl Compounds / Q000378:metabolism
D019837:Ryanodine Receptor Calcium Release Channel; D013438:Sulfhydryl Compounds; D005978:Glutathione