title: Functional brain mapping in freely moving rats during treadmill walking.
abstract: A dilemma in functional neuroimaging is that immobilization of the subject, necessary to avoid movement artifact, extinguishes all but the simplest behaviors. Recently, we developed an implantable microbolus infusion pump (MIP) that allows bolus injection of radiotracers by remote activation in freely moving, nontethered animals. The MIP is examined as a tool for brain mapping in rats during a locomotor task. Cerebral blood flow-related tissue radioactivity (CBF-TR) was measured using [14C]-iodoantipyrine with an indicator-fractionation method, followed by autoradiography. Rats exposed to walking on a treadmill, compared to quiescent controls, showed increases in CBF-TR in motor circuits (primary motor cortex, dorsolateral striatum, ventrolateral thalamus, midline cerebellum, copula pyramis, paramedian lobule), in primary somatosensory cortex mapping the forelimbs, hindlimbs and trunk, as well as in secondary visual cortex. These results support the use of implantable pumps as adjunct tools for functional neuroimaging of behaviors that cannot be elicited in restrained or tethered animals.
authors.affiliation: Department of Psychiatry and the Behavioral Sciences, University of Southern California, Keck School of Medicine, Los Angeles, California, U.S.A. holschne@usc.edu
authors.forename: D P
authors.initials: DP
authors.lastname: Holschneider
authors.forename: J-M I
authors.initials: JM
authors.lastname: Maarek
authors.forename: J
authors.initials: J
authors.lastname: Yang
authors.forename: J
authors.initials: J
authors.lastname: Harimoto
authors.forename: O U
authors.initials: OU
authors.lastname: Scremin
mesh_terms: D000818:Animals; D000983:Antipyrine / Q000031:analogs & derivatives*; D001931:Brain Mapping / Q000379:methods*; D002250:Carbon Radioisotopes; D005080:Exercise Test; D015918:Infusion Pumps, Implantable; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002250:Carbon Radioisotopes; D000983:Antipyrine; C008135:iodoantipyrine