title: [Investigation of connective tissue growth factor's gene cloning].
abstract: OBJECTIVE
  The human connective tissue growth factor(CTGF), as the responsive product of immediate early gene, was cloned for the investigation of cellular primary gene responded and healing repairing.
  
  
  METHODS
  RT-PCR was performed with the mRNA of HUVE cell stimulated, the three clones of CTGF, via mutational modified, were ligated to a complete gene that identified with sequencing.
  
  
  RESULTS
  The sequencing indicated that this CTGF gene contains complete coding region and the 3'-noncoding region which is difference with the reported sequence.
  
  
  CONCLUSIONS
  A new human CTGF gene was cloned.
authors.affiliation: Department of Viral and Immunology, Institute of Medical Biology, CAMS, PUMC, Kunming 650118, China. glimbcam@km.ym.cn
authors.forename: Q
authors.initials: Q
authors.lastname: Li
authors.forename: H
authors.initials: H
authors.lastname: Zhao
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Fu
authors.forename: S
authors.initials: S
authors.lastname: Zhao
authors.forename: L
authors.initials: L
authors.lastname: Jiang
mesh_terms: D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D055513:Connective Tissue Growth Factor; D017781:Genes, Immediate-Early / Q000235:genetics*; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics*; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014471:Umbilical Veins / Q000166:cytology
chemical_list: C526317:CCN2 protein, human; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D055513:Connective Tissue Growth Factor