title: Capillary electrochromatographic separation of enantiomers on chemically bonded type of cellulose derivative chiral stationary phases with a positively charged spacer.
abstract: Positively charged chiral stationary phases (CSPs) were prepared for capillary electrochromatography (CEC) separation of enantiomers by chemically immobilizing cellulose derivatives onto diethylenetriaminopropylated silica (DEAPS) with tolylene-2,4-diisocyanate (TDI) as a spacer reagent. Anodic electroosmotic mobility was observed in both nonaqueous and aqueous mobile phases due to the positively charged amines on the surface of the prepared CSPs. For comparison, the traditionally used 3-aminopropyl silica (APS) was also adopted as the base material instead of DEAPS to prepare CSP. It was observed that the EOF on the DEAPS-based CSP was 18%-60% higher than that on the APS-based CSP under nonaqueous mobile phase conditions. Separation of enantiomers in CEC was performed on the positively charged CSPs with the nonaqueous mobile phases of pure ethanol or mixture of hexane-alcohol and the aqueous phases of acetonitrile-water or 95% ethanol. Fast separation of enantiomers was achieved on the newly prepared CSPs.
authors.affiliation: National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116 011, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Chen
authors.forename: Wenhai
authors.initials: W
authors.lastname: Jin
authors.forename: Feng
authors.initials: F
authors.lastname: Qin
authors.forename: Yueqi
authors.initials: Y
authors.lastname: Liu
authors.forename: Hanfa
authors.initials: H
authors.lastname: Zou
authors.forename: Baochuan
authors.initials: B
authors.lastname: Guo
mesh_terms: D002482:Cellulose; D002845:Chromatography / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods*; D006571:Heterocyclic Compounds / Q000302:isolation & purification; D004364:Pharmaceutical Preparations / Q000302:isolation & purification; D012822:Silicon Dioxide; D013237:Stereoisomerism
chemical_list: D006571:Heterocyclic Compounds; D004364:Pharmaceutical Preparations; D012822:Silicon Dioxide; D002482:Cellulose