Comparison between fluorescence in situ hybridization (FISH) and quantitative-fluorescent polymerase chain reaction (QF-PCR) for the detection of aneuploidies in single blastomeres.
OBJECTIVE
The aim of our investigation was to compare the efficiencies of the fluorescence in situ hybridization (FISH) and the quantitative-fluorescent PCR (QF-PCR) methods for the detection of sexing and numerical chromosome disorders in single blastomeres collected from the same preimplantation human embryos.


METHODS
FISH analysis was carried out on 145 blastomeres from the 79 surplus embryos with probes specific for chromosomes 13, 18, 21, X, and Y. QF-PCR was performed with each one or two of the primers specific for the same chromosomes on 151 blastomeres from the same embryos obtained from patients undergoing IVF treatment.


RESULTS
Analyses were possible on 135 blastomeres (93%) by FISH and on 117 blastomeres (77%) by QF-PCR. Of 65 embryos, which could be analyzed by both methods, 20 embryos (31%) were diagnosed as abnormal.


CONCLUSIONS
The present study shows that FISH tests are more accurate than QF-PCR assays for the detection of numerical chromosome disorders when performed on single blastomeres.
Sato
Takeshi
T
Department of Obstetrics and Gynaecology, Graduate School of Medical Science, Nagoya City University, Nagoya, Japan. og.sato@med.nagoya-cu.ac.jp
Ikuta
Katsuo
K
Sherlock
Jon
J
Adinolfi
Matteo
M
Suzumori
Kaoru
K
D000782:Aneuploidy; D001757:Blastomeres / Q000648:ultrastructure*; D015342:DNA Probes; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D009006:Monosomy; D009030:Mosaicism; D016133:Polymerase Chain Reaction / Q000379:methods*; D019836:Preimplantation Diagnosis / Q000379:methods*; D014314:Trisomy
D015342:DNA Probes