title: Expression of stem cell factor and c-kit in human malignant fibrous histiocytoma cell line (TNMY1).
abstract: BACKGROUND
  The expression of c-kit and/or its ligand, stem cell factor (SCF), has been related to tumor proliferation, in several tumor systems.
  
  
  METHODS
  We analyzed the expression of the SCF/its receptor (c-kit) mRNA and the production of soluble SCF in a human malignant fibrous histiocytoma (MFH) cell line (TNMY1).
  
  
  RESULTS
  Immunocytochemical analysis revealed that the TNMY1 cells were positive for both SCF and c-kit. Reverse transcriptase-polymerase chain reaction (RT-PCR) showed that the TNMY1 cell line expressed mRNA for SCF and c-kit. By using an enzyme-linked immunosorbent assay (ELISA), the TNMY1 cells were found to produce relatively high amounts of soluble SCF. However, the addition of soluble SCF to the TNMY1 cells did not alter the in vitro growth ability of the cells.
  
  
  CONCLUSIONS
  Our data showed that the MFH cells produced consistent amounts of SCF but did not demonstrate autocrine growth modulation. Thus, SCF secretion may have a paracrine activity in the growth of MFH cells.
authors.affiliation: Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe, Japan. Tetsuya.Nakatani@ma9.seikyou.ne.jp
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Nakatani
authors.forename: Takashi
authors.initials: T
authors.lastname: Marui
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Hitora
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Akisue
authors.forename: Teruya
authors.initials: T
authors.lastname: Kawamoto
authors.forename: Keiko
authors.initials: K
authors.lastname: Nagira
authors.forename: Ikuo
authors.initials: I
authors.lastname: Fujita
authors.forename: Keiji
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Shinichi
authors.initials: S
authors.lastname: Yoshiya
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kurosaka
mesh_terms: D002455:Cell Division / Q000502:physiology; D018219:Histiocytoma, Benign Fibrous / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D019009:Proto-Oncogene Proteins c-kit / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D019089:Stem Cell Factor / Q000096:biosynthesis* / Q000235:genetics; D014407:Tumor Cells, Cultured
chemical_list: D012333:RNA, Messenger; D019089:Stem Cell Factor; D019009:Proto-Oncogene Proteins c-kit