[Induced expression of dopamine D2 receptor in human neuro-progenitor cell line HNG1210-E].
OBJECTIVE
To investigate whether the dopamine receptor D2 can express or can be induced to express in HNG1210-E, a monoclonal, telomerase-immortalized, human neural progenitor cell line.


METHODS
By means of RT-PCR, immuno-fluorescent staining, and fluo3 Ca2+ imaging to the expression of D2 mRNA and protein as well as the reaction to dopamine were demonstrated.


RESULTS
HNG1210-E cells could be induced to express D2 mRNA and its proteins. The induced cells also reacted to dopamine (5 mmol.L-1), which caused rapid rising of cytoplasm Ca2+.


CONCLUSIONS
HNG1210-E cells can be induced to express D2 mRNA and functional proteins.
Hu
Qikuan
Q
Peking University Stem Cell Research Center, Beijing 100083, China.
Lin
Changsheng
C
Bai
Yun
Y
Bai
Jie
J
He
Qihua
Q
Shen
Li
L
Li
Lingsong
L
D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D003593:Cytoplasm / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D006801:Humans; D009474:Neurons / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D017448:Receptors, Dopamine D2 / Q000032:analysis / Q000235:genetics*; D013234:Stem Cells / Q000378:metabolism*
D012333:RNA, Messenger; D017448:Receptors, Dopamine D2; D002118:Calcium