Inhibition of apoptosis by recombinant 30K protein originating from silkworm hemolymph.
In a previous study, we reported that silkworm hemolymph inhibits apoptosis and that the anti-apoptotic component in silkworm hemolymph is a 30K protein. In this study, the 30K protein encoded by 30Kc6 was expressed in Escherichia coli. The recombinant 30K protein was expressed as an inclusion body, and the inclusion body was separated and refolded by affinity column chromatography using a 6xHis tag. We demonstrated that apoptosis is inhibited by supplementing the culture medium with this purified recombinant 30K protein. The recombinant 30K protein inhibited the virus- or chemical-induced apoptosis in human cells as well as insect cells. Apoptosis-inhibitory activity of recombinant 30K protein was comparable to that of whole silkworm hemolymph. The recombinant 30K protein can be effectively used to minimize cell death and consequently increase the productivity by extending the production time of host cells in commercial animal cell culture.
Kim
Eun Jeong
EJ
School of Chemical Engineering and Institute of Chemical Processes, Seoul National University, Gwanak-Gu Shilim-Dong San 56-1, South Korea.
Park
Hye Jung
HJ
Park
Tai Hyun
TH
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D012831:Bombyx / Q000737:chemistry*; D002460:Cell Line; D004926:Escherichia coli / Q000235:genetics; D006458:Hemolymph / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry / Q000494:pharmacology; D016415:Sequence Alignment; D018411:Spodoptera; D019311:Staurosporine / Q000037:antagonists & inhibitors
D011994:Recombinant Proteins; D019311:Staurosporine