Metabolism of dynorphins by peptidases of pulmonary artery endothelial cells.
Degradation of several dynorphins by peptidases expressed in cultured porcine pulmonary artery endothelial cells was studied by incubation of the peptide in cell suspensions followed by electrospray ionization and tandem mass spectrometric analyses. Under the in vitro conditions applied, only the metabolism of dynorphin A1-8 occurred in a significant extent. Studies involving specific peptidase inhibitors indicated that mainly bestatin-sensitive aminopeptidases, thiorphan-sensitive endopeptidases, and cFPAAF-pAB-sensitive endopeptidases expressed by the endothelial cells were involved in the process that converted dynorphin A1-8 to dynorphin A2-8, dynorphin A1-6, and leucine enkephalin (dynorphin A1-5), respectively. These peptidases may form a metabolic barrier for the cellular penetration of intact dynorphin A1-8 and/or control effects of the circulating peptide on endothelial opioid receptors of the cells.
Zharikova
Alevtina
A
Center for Drug Discovery, College of Pharmacy, University of Florida, Health Science Center, Gainesville, FL 32610-0497, USA.
Zharikov
Sergey
S
Block
Edward R
ER
Prokai
Laszlo
L
D000818:Animals; D002478:Cells, Cultured; D004399:Dynorphins / Q000737:chemistry / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology / Q000201:enzymology*; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism*; D011480:Protease Inhibitors / Q000494:pharmacology; D011651:Pulmonary Artery / Q000166:cytology / Q000201:enzymology*; D021241:Spectrometry, Mass, Electrospray Ionization; D013552:Swine
D010446:Peptide Fragments; D011480:Protease Inhibitors; D004399:Dynorphins; D010447:Peptide Hydrolases