Two-laser infrared and ultraviolet matrix-assisted laser desorption/ionization.
Matrix-assisted laser desorption/ionization (MALDI) was performed using two pulsed lasers with wavelengths in the IR and UV regions. A 10.6 micro m pulsed CO(2) laser was used to irradiate a MALDI target, followed after an adjustable delay by a 337 nm pulsed nitrogen laser. The sample consisted of a 2,5-dihydroxybenzoic acid matrix and bovine insulin guest molecule. The pulse energy for both of the lasers was adjusted so that the ion of interest, either the matrix or guest ion, was not produced by either of the lasers alone. The delay time for maximum ion yield occurs at 1 micro s for matrix and guest ions and the signal decayed to zero in approximately 400 micro s. A mechanism is presented for enhanced UV MALDI ion yield following the IR laser pulse based on transient heating.
Little
Mark W
MW
Department of Chemistry, Louisiana State University, Baton Rouge, Louisiana 70803, USA.
Kim
Jae-Kuk
JK
Murray
Kermit K
KK
D000818:Animals; D002417:Cattle; D007259:Infrared Rays; D007328:Insulin / Q000032:analysis; D007834:Lasers; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation* / Q000379:methods; D014466:Ultraviolet Rays
D007328:Insulin