title: Intra-individual variation of basal metabolic rate and the influence of daily habitual physical activity before testing.
abstract: The present study determined the intra-individual variation of BMR measurements, using a standard out-patient protocol, with the subjects transporting themselves to the laboratory for the BMR measurements after spending the night at home. The effect of a non-fasting state and variation in daily habitual physical activity the day before testing was evaluated. Eight male and eleven female subjects participated in three BMR measurements with 2-week intervals. Physical activity was estimated with a tri-axial accelerometer for movement registration, during the 3 d before each BMR measurement. There were no significant differences in estimated BMR (ANOVA repeated measures, P=0.88) and in physical activity (ANOVA repeated measures, P=0.21). Mean within-subject CV in BMR was found to be 3.3 (SD 2.1) %, ranging from 0.4 to 7.2 %. Differences between BMR measurements could not be explained by differences in physical activity the day before; however the mean within-subject CV in BMR changed from 5.7 to 5.2 % after correcting for within-machine variability and from 5.2 to 3.3 % after excluding five measurements because of non-compliance to the protocol including fasting. In conclusion, BMR values measured with a standard out-patient protocol are sufficiently reproducible for most practical purposes despite the within-subject variability in physical activity the day before the measurement. For this purpose, however, non-fasting subjects must be excluded and a regular function check of the ventilated-hood system is recommendable.
authors.affiliation: Department of Human Biology, Maastricht University, PO Box 616, 6200 MD Maastricht, The Netherlands. M.adriaens@HB.Unimaas.nl
authors.forename: Mirjam P E
authors.initials: MP
authors.lastname: Adriaens
authors.forename: Paul F M
authors.initials: PF
authors.lastname: Schoffelen
authors.forename: Klaas R
authors.initials: KR
authors.lastname: Westerterp
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D001481:Basal Metabolism / Q000502:physiology*; D001823:Body Composition; D002151:Calorimetry; D002985:Clinical Protocols; D004734:Energy Metabolism / Q000502:physiology; D015444:Exercise / Q000502:physiology*; D005215:Fasting / Q000502:physiology; D005260:Female; D006801:Humans; D008297:Male; D010349:Patient Compliance; D015203:Reproducibility of Results