title: [Molecular cloning of Plasmodium yoelii dynamin-like protein (PyDyn) gene and the immunological character of its domains].
abstract: OBJECTIVE
  To identify and clone a new full ORF gene of PyDyn (Plasmodium yoelii dynamin-like protein), and examine the protection of their expression products.
  
  
  METHODS
  Using the P. yoelii Genome technology and RT-PCR.
  
  
  RESULTS
  The full ORF gene of PyDyn was amplified from mRNA of the erythrocytic stage of P. yoelii., three domains of PyDyn were expressed in E. coli., and the fairly positive immunogenicity of them was showed by IFA. The full ORF gene of PyDyn was 2,433 bp and encode 811 amino acids. Its Gene Bank access number is AF458071. PyDyn belongs to the dynamin-like protein family according to its property.
  
  
  CONCLUSIONS
  The new full ORF gene of PyDyn is obtained and identified; their expressed domains are probably new candidates for malaria vaccine.
authors.affiliation: Department of Etiology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
authors.forename: Ying-hong
authors.initials: YH
authors.lastname: Mao
authors.forename: Heng
authors.initials: H
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D034281:Dynamins / Q000235:genetics* / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics; D017125:Genes, Protozoan / Q000235:genetics / Q000276:immunology*; D017780:Malaria Vaccines / Q000276:immunology; D008969:Molecular Sequence Data; D010967:Plasmodium yoelii / Q000737:chemistry / Q000235:genetics*; D015800:Protozoan Proteins / Q000235:genetics* / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D017780:Malaria Vaccines; D015800:Protozoan Proteins; D014614:Vaccines, Synthetic; D034281:Dynamins