[The influences of human apolipoprotein E mutants on the learning and memory of cerebral cortex in transgenic mice].
OBJECTIVE
To study the influences of the mutants of human apolipoprotein E on the function of cerebral cortex.


METHODS
The Southern blot hybridization and ELISA techniques were used for identification of integration and expression of human apoE4 and apoE7 genes at the mice chromosome and in the serum of F1 transgenic mice. Then the active avoidance response test was performed to determine the alteration of learning and short term and long term memory of the transgenic mice. The serum total cholesterol (TC) and triglyceride (TG) were determined simultaneously with enzymatic procedure.


RESULTS
(1) In F1 transgenic mice the human apoE genes integrated at the chromosome and expressed in their serum stably. (2) In apoE4 mice the learning and short term memorizing abilities were decreased, and in apoE7 mice the short term memorizing abilities were decreased as well. In addition, the TC and TG increased statistically.


CONCLUSIONS
The overexpression of human apoE4 and apoE7 genes in transgenic mice could damage the function of cerebral cortex. It denoted that the mutations of apoE might correlate the pathogenesis of Alzheimer's disease.
Liao
Z
Z
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China.
Qi
Z
Z
Sun
M
M
Yang
D
D
Yun
J
J
D000818:Animals; D053318:Apolipoprotein E3; D053327:Apolipoprotein E4; D001057:Apolipoproteins E / Q000235:genetics / Q000502:physiology*; D002540:Cerebral Cortex / Q000502:physiology*; D002784:Cholesterol / Q000097:blood; D006801:Humans; D007858:Learning / Q000502:physiology*; D008568:Memory / Q000502:physiology*; D051379:Mice; D008822:Mice, Transgenic; D009154:Mutation; D014280:Triglycerides / Q000097:blood
D053318:Apolipoprotein E3; D053327:Apolipoprotein E4; D001057:Apolipoproteins E; D014280:Triglycerides; C049579:apolipoprotein E7, human; D002784:Cholesterol