title: Structure of human phosphopantothenoylcysteine synthetase at 2.3 A resolution.
abstract: The structure of human phosphopantothenoylcysteine (PPC) synthetase was determined at 2.3 A resolution. PPC synthetase is a dimer with identical monomers. Some features of the monomer fold resemble a group of NAD-dependent enzymes, while other features resemble the ribokinase fold. The ATP, phosphopantothenate, and cysteine binding sites were deduced from modeling studies. Highly conserved ATP binding residues include Gly43, Ser61, Gly63, Gly66, Phe230, and Asn258. Highly conserved phosphopantothenate binding residues include Asn59, Ala179, Ala180, and Asp183 from one monomer and Arg55' from the adjacent monomer. The structure predicts a ping pong mechanism with initial formation of an acyladenylate intermediate, followed by release of pyrophosphate and attack by cysteine to form the final products PPC and AMP.
authors.affiliation: Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Narayanan
authors.initials: N
authors.lastname: Manoj
authors.forename: Erick
authors.initials: E
authors.lastname: Strauss
authors.forename: Tadhg P
authors.initials: TP
authors.lastname: Begley
authors.forename: Steven E
authors.initials: SE
authors.lastname: Ealick
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002472:Cell Transformation, Viral; D017124:Conserved Sequence; D018360:Crystallography, X-Ray; D019281:Dimerization; D004926:Escherichia coli / Q000201:enzymology; D005822:Genetic Vectors; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010453:Peptide Synthases / Q000138:chemical synthesis / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000737:chemistry; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D013059:Spectrum Analysis, Raman; D013379:Substrate Specificity; D014027:Tobacco Mosaic Virus / Q000235:genetics
chemical_list: D017853:Phosphotransferases (Alcohol Group Acceptor); C051254:hydroxyethylthiazole kinase; D010453:Peptide Synthases; C022374:phosphopantothenoyl-cysteine synthetase