Salt-dependent folding of long duplex DNA by histone H1.
It is found that T4 phage DNA complexed with histone H1 assembled into a string-of-bead structure, when the complex is prepared by a gentle diluting procedure from a high salt solution (2 M NaCl) to a low salt solution (50 mM NaCl). We used fluorescence microscopy to perform the real-time observation on formation and motion of a string-of-bead structure. Spatial histone H1 distribution on the DNA-H1 complex is observed by immuno-fluorescence microscopy.
Yoshikawa
Y
Y
Department of Food and Nutrition, Nagoya Bunri College, 2-1 Sasazuka, Nishi-ku, Nagoya 451-0077, Japan.
Kanbe
T
T
D017122:Bacteriophage T4 / Q000737:chemistry; D004279:DNA, Viral / Q000737:chemistry* / Q000187:drug effects* / Q000648:ultrastructure; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D006657:Histones / Q000494:pharmacology*; D007211:Indoles; D008856:Microscopy, Fluorescence; D009690:Nucleic Acid Conformation / Q000187:drug effects; D012965:Sodium Chloride / Q000494:pharmacology
D004279:DNA, Viral; D005456:Fluorescent Dyes; D006657:Histones; D007211:Indoles; D012965:Sodium Chloride; C007293:DAPI; D016650:Fluorescein-5-isothiocyanate