title: Posttranscriptional modification of transfer RNA in the submarine hyperthermophile Pyrolobus fumarii.
abstract: In the RNA of hyperthermophiles, which grow optimally between 80 degrees C and 106 degrees C, posttranscriptional modification has been identified as a leading mechanism of structural stabilization. Particularly in the Archaeal evolutionary domain these modifications are expressed as a structurally diverse array of modification motifs, many of which include ribose methylation. Using mass spectrometric techniques we have examined the posttranscriptional modifications in unfractionated tRNA from the remarkable organism Pyrolobus fumarii, which grows optimally at 106 degrees C, but up to 113 degrees C (Blöchl et al. (1997), Extremophiles, 1, 14-21). Twenty-six modified nucleosides were detected, 11 of which are methylated in ribose. A new RNA nucleoside, 1,2'-O-dimethylguanosine (m1Gm) was characterized and the structure confirmed by chemical synthesis.
authors.affiliation: Department of Medicinal Chemistry, University of Utah, Salt Lake City, UT 84112, USA.
authors.forename: J A
authors.initials: JA
authors.lastname: McCloskey
authors.forename: X H
authors.initials: XH
authors.lastname: Liu
authors.forename: P F
authors.initials: PF
authors.lastname: Crain
authors.forename: E
authors.initials: E
authors.lastname: Bruenger
authors.forename: R
authors.initials: R
authors.lastname: Guymon
authors.forename: T
authors.initials: T
authors.lastname: Hashizume
authors.forename: K O
authors.initials: KO
authors.lastname: Stetter
mesh_terms: D002851:Chromatography, High Pressure Liquid; D019615:Crenarchaeota / Q000378:metabolism*; D006151:Guanosine / Q000031:analogs & derivatives / Q000737:chemistry; D006358:Hot Temperature; D013058:Mass Spectrometry; D008745:Methylation; D015394:Molecular Structure; D012323:RNA Processing, Post-Transcriptional; D019642:RNA, Archaeal / Q000737:chemistry* / Q000378:metabolism*; D012343:RNA, Transfer / Q000737:chemistry* / Q000378:metabolism*; D012263:Ribonucleosides / Q000737:chemistry
chemical_list: D019642:RNA, Archaeal; D012263:Ribonucleosides; D006151:Guanosine; D012343:RNA, Transfer