Mutagenesis targeted by triple-helix forming oligonucleotides containing a reactive nucleoside analogue.
Recently, we have demonstrated that 2-amino-6-vinypurine derivative (1) achieves triplex-forming cross-linking with high selectivity toward the cytosine of the G-C target site. In this study, we have investigated the cross-linking as well as induction of point mutations with the TFO incorporating 1 to a target site in a shuttle vector plasmid that replicates in mammalian cells. It was revealed that the TFO bearing 1 introduced mutations at the site of cross-linking. These results have suggested that the selective cross-linking with 1 might be useful for development of new biotechnology for targeted-mutagenesis.
Nagatsugi
Fumi
F
Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, CREST (JST), Japan.
Sasaki
Shigeki
S
Seidmen
Michael M
MM
Miller
Paul S
PS
D000818:Animals; D001483:Base Sequence; D016153:Genes, Suppressor; D016296:Mutagenesis; D009711:Nucleotides / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D012343:RNA, Transfer / Q000737:chemistry / Q000235:genetics
D009711:Nucleotides; D009841:Oligonucleotides; C096275:supF tRNA; D012343:RNA, Transfer