A combined HNCA/HNCO experiment for 15N labeled proteins with 13C at natural abundance.
A triple resonance NMR experiment is presented for the simultaneous recording of HNCA and HNCO data sets on (15)N, natural abundance (13)C samples. The experiment exploits the fact that transfers of magnetization from (15)N to (13)CO and from (15)N to (13)C(alpha) (and back) proceed independently for samples that are not enriched in (13)C. A factor of 2 in measuring time is gained by recording the two data sets simultaneously with no compromise in spectral quality. An application to a 0.5 mM (15)N labeled sample of protein-L is presented with all expected correlations observed in spectra recorded with a cryogenic probe at 500 MHz.
Kupce
Eriks
E
Varian Inc., Surrey KT12 2QF, U.K.
Muhandiram
D R
DR
Kay
Lewis E
LE
D002247:Carbon Isotopes; D009682:Magnetic Resonance Spectroscopy; D009587:Nitrogen Isotopes; D011506:Proteins / Q000737:chemistry*; D013194:Staining and Labeling
D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D011506:Proteins