Association of uteroglobulin-related protein 1 with bronchial asthma.
BACKGROUND
Chromosomal region 5q31 harbours a number of genes associated with atopic phenotypes, for example the genes coding for interleukin (IL) 4, IL13 and the beta(2)-adrenoreceptor. A new gene within this region was identified only very recently. The encoded protein - uteroglobin-related protein 1 (UGRP1) - is thought to act as an anti-inflammatory agent and is mainly expressed in the lung and trachea. The functional promoter polymorphism A-112G in UGRP1 was shown to be associated with bronchial asthma in a Japanese population. We were thus interested in finding out whether the polymorphism so far identified or others within UGRP1 were associated with bronchial asthma in a German Caucasian population.


METHODS
We performed direct genomic sequencing of the promoter and coding region of UGRP1 in 15 asthmatic children and 15 controls. The identified polymorphisms were genotyped by means of RFLP. Statistical analysis was performed with the Armitage trend test.


RESULTS
We identified 5 non-coding variants, 4 of which being described for the first time. Three polymorphisms were common and typed in 182 asthmatic children and 270 controls. None of the polymorphisms were associated with bronchial asthma in our population.


CONCLUSIONS
We conclude from our data that UGRP1 does not play a major role in the development of bronchial asthma in our Caucasian population.
Heinzmann
Andrea
A
University Children's Hospital, University of Freiburg, Freiburg, Germany. heinzmann@kikli.ukl.uni-freiburg.de
Dietrich
Henriette
H
Deichmann
Klaus A
KA
D000293:Adolescent; D001249:Asthma / Q000235:genetics* / Q000276:immunology; D002352:Carrier Proteins; D002648:Child; D002675:Child, Preschool; D004247:DNA / Q000737:chemistry / Q000235:genetics; D008040:Genetic Linkage; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D060765:Secretoglobins; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics* / Q000276:immunology; D014598:Uteroglobin
D002352:Carrier Proteins; C449940:SCGB3A2 protein, human; D060765:Secretoglobins; D014157:Transcription Factors; D004247:DNA; D014598:Uteroglobin