[Comparative study of the expression and phosphorylation of neurofilament proteins of brain gray matter in Alzheimer's disease].
OBJECTIVE
To explore the role of abnormal modification of neurofilament proteins (NFPs) in the pathogenesis of Alzheimer disease (AD), and the characteristics of NFPs in distribution and solubility.


METHODS
Ultra-centrifugation and Western blot were utilized to isolate and analyze NF subunits. Ultra-centrifugation and Western blot were utilized to observe the effect of abnormal modification of neurofilament proteins (NFPs) on the characteristics of NFPs in distribution and solubility in the pathogenesis of Alzheimer disease.


RESULTS
In human brain gray matter, NF subunits were mainly detected in particular fraction. Compared with Huntington's disease (HD), the content of NF subunits was increased in AD brain gray matter and the increased level of NF-H and NF-M were mainly in abnormal hyper-phosphorylate form.


CONCLUSIONS
Abnormal phosphorylation and accumulation of NFPs are involved in pathology of AD brain.
Wang
Y P
YP
Department of Pathophysiology, Tongji Medical University, Wuhan 430030, China. wyipeng@yahoo.com
Wei
Z L
ZL
Wang
X C
XC
Wang
Q
Q
Wang
J Z
JZ
D000544:Alzheimer Disease / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D006801:Humans; D006816:Huntington Disease / Q000378:metabolism; D016900:Neurofilament Proteins / Q000378:metabolism*; D010766:Phosphorylation
D016900:Neurofilament Proteins