Transcription regulatory cis-element (GCC)8 in the 5'-untranslated region of the gene for human very-low-density lipoprotein receptors.
The 5'-untranslated region of very-low-density lipoprotein (VLDL) receptor gene includes 2 groups of triplet repeats (GCC)n. Four repeats are localized near the promoter region in position 15.23 from the transcription initiation site. Eight repeats were detected in position 573.597. Sequence (GCC)8 in VLDL receptor gene forms specific complexes with nuclear proteins of HepG2 cells, the formation of these complexes depended on Zn(2+). Superexpression of the CGGBP-20 protein interacting with long sequences (GCC)n suppressed transcriptional activity of VLDL receptor gene. Removal of fragment (397.616) containing cis-element (GCC)8 from the 5'-untranslated region of VLDL receptor gene led to activation of the linked marker gene cat in Hutu80 cells, but did not abolish the repressor effect of CGGBP-20 protein. Our results suggest that (GCC)n-binding proteins differing from CGGBP-20 regulate activity of the VLDL receptor gene via cis-element (GCC)8.
Kuteikin-Teplyakov
K B
KB
Institute of Experimental Medicine, Russian Academy of Medical Sciences, St Petersburg.
Orlov
S V
SV
Dizhe
E B
EB
Perevozchikov
A P
AP
D020121:5' Untranslated Regions / Q000235:genetics*; D000818:Animals; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D005809:Genes, Regulator; D006801:Humans; D011973:Receptors, LDL / Q000235:genetics*; D014158:Transcription, Genetic; D018911:Trinucleotide Repeats; D015032:Zinc / Q000378:metabolism
D020121:5' Untranslated Regions; D004268:DNA-Binding Proteins; D011973:Receptors, LDL; C036053:VLDL receptor; D015032:Zinc