Effect of coxsackievirus b3 on ion channel currents in rat ventricular myocytes.
OBJECTIVE
To investigate the effects of coxsackievirus B3(CVB3) on ion channel currents in rat ventricular myocytes.


METHODS
Rat hearts were isolated with collagenase to acquire single ventricular myocytes, L-type voltage-dependent calcium channel (VDCC) current (I(Ca)), Na+ current (I(Na)), outward potassium current (I(out)), inwardly rectifying potassium current(I(KI)) were recorded using whole cell patch clamp techniques.


RESULTS
CVB3 infection increased I(Ca) and I(out), while decreased I(KI); but it had no obvious effect on I(Na).


CONCLUSIONS
The effects of CVB3 an I(Ca), I(out), I(KI) may be one of the mechanisms of myocytes damage and the occurrence of abnormal electroactivities induced by CVB3 infection.
Bao
W
W
Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital of Shanghai Medical University, Shanghai 200032.
Liu
G
G
Wang
C
C
Yang
Y
Y
Guo
Q
Q
Yu
Y
Y
D000818:Animals; D000831:Animals, Newborn; D020746:Calcium Channels, L-Type; D002469:Cell Separation; D029822:Enterovirus B, Human / Q000472:pathogenicity; D004769:Enterovirus Infections / Q000503:physiopathology*; D006352:Heart Ventricles / Q000821:virology; D007473:Ion Channels; D032383:Myocytes, Cardiac / Q000502:physiology* / Q000821:virology; D018408:Patch-Clamp Techniques; D024661:Potassium Channels, Inwardly Rectifying; D051381:Rats; D017207:Rats, Sprague-Dawley; D015222:Sodium Channels; D016276:Ventricular Function
D020746:Calcium Channels, L-Type; D007473:Ion Channels; D024661:Potassium Channels, Inwardly Rectifying; D015222:Sodium Channels