[Hemangioblastic characteristics of fetal bone marrow-derived Flk1+ CD34- cells].
OBJECTIVE
To investigate whether fetal bone marrow stromal cells have hemangioblastic characteristics.


METHODS
Human fetal bone marrow stromal cells (hfMSCs) were isolated and cultured. Immunophenotypes of hfMSCs were tested by FACS. hfMSCs seeded in the matrigel were induced with vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in vitro. Vascularization and hematopoiesis were detected with immunohistochemistry and electron microscope.


RESULTS
The typical properties of this CD34- stromal cell population were that 99% cells expressed Flk1 (vascular endothelial cell growth factor receptor 2) and tube structure was formed. In the process of induction, hfMSCs could give rise to CD34+ round cells.


CONCLUSIONS
We have demonstrated that fetal bone marrow stroma-derived Flk1+ CD34- cells could differentiate into vascular endothelial cells and hematopoietic cells, indicating that fetal bone marrow stroma-derived Flk1+ CD34- cells have hemangioblastic characteristics.
Guo
Hong
H
State Key Laboratory of Experimental Hematology, Institute of Hematology, CAMS, PUMC, Tianjin 300020, China.
Zhao
Zhi-gang
ZG
Liu
Jie-wen
JW
Chen
Hui-shu
HS
Zhao
Chun-hua
CH
D018952:Antigens, CD34 / Q000276:immunology*; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D005333:Fetus; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D006801:Humans; D016130:Immunophenotyping; D017154:Stromal Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000502:physiology*
D018952:Antigens, CD34; D016222:Fibroblast Growth Factor 2; D040301:Vascular Endothelial Growth Factor Receptor-2