title: Exposure and sensitization to indoor allergens: association with lung function, bronchial reactivity, and exhaled nitric oxide measures in asthma.
abstract: BACKGROUND
  Exposure to high levels of allergens in sensitized asthmatic patients causes worsening of pulmonary function in experimental studies. Chronic exposure to lower, naturally occurring levels of allergens might increase the severity of asthma.
  
  
  OBJECTIVE
  We sought to study the associations between sensitization and exposure to common indoor allergens (dust mite, cat, and dog) in the home on pulmonary function, exhaled nitric oxide (eNO), and airway reactivity in asthmatic patients.
  
  
  METHODS
  Dust samples were collected from the living room carpet and mattress of 311 subject's homes, and Der p 1, Fel d 1, and Can f 1 concentrations were measured by using ELISAs. Spirometry, nonspecific bronchial reactivity, and eNO were measured.
  
  
  RESULTS
  Subjects both sensitized and exposed to high levels of sensitizing allergen had significantly lower FEV(1) percent predicted values (mean, 83.7% vs 89.3%; mean difference, 5.6%; 95% CI, 0.6%-10.6%; P =.03), higher eNO values (geometric mean [GM], 12.8 vs 8.7 ppb; GM ratio, 0.7; 95% CI, 0.5-0.8; P =.001), and more severe airways reactivity (PD(20) GM, 0.25 vs 0.73 mg; GM ratio, 2.9; 95% CI, 1.6-5.0; P <.001) compared with subjects not sensitized and exposed. No significant effect of the interaction between sensitization and exposure was found for FEV(1) percent predicted and eNO values. However, there was a significant effect of the interaction between sensitization and exposure to any allergen (P =.05) and between sensitization and exposure to cat allergen (P =.04) for nonspecific bronchial reactivity.
  
  
  CONCLUSIONS
  Asthmatic subjects who are exposed in their homes to allergens to which they are sensitized have a more severe form of the disease.
authors.affiliation: North West Lung Centre, Wythenshawe Hospital, Manchester, United Kingdom.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Langley
authors.forename: Sophie
authors.initials: S
authors.lastname: Goldthorpe
authors.forename: Mark
authors.initials: M
authors.lastname: Craven
authors.forename: Julie
authors.initials: J
authors.lastname: Morris
authors.forename: Ashley
authors.initials: A
authors.lastname: Woodcock
authors.forename: Adnan
authors.initials: A
authors.lastname: Custovic
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016902:Air Pollution, Indoor; D000485:Allergens / Q000032:analysis / Q000276:immunology*; D001249:Asthma / Q000150:complications / Q000276:immunology / Q000503:physiopathology*; D016535:Bronchial Hyperreactivity / Q000209:etiology; D002648:Child; D004781:Environmental Exposure; D005260:Female; D006798:Housing; D006801:Humans; D007114:Immunization; D008168:Lung / Q000503:physiopathology*; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide; D012119:Respiration; D012129:Respiratory Function Tests
chemical_list: D000485:Allergens; D009569:Nitric Oxide