Laser-assisted microinjection into targeted animal cells.
A pulsed (17 nanoseconds) Nd:YAG laser (1064 nm) was used to inject impermeable dyes (propidium iodide andiodide and merocyanine 540) and a plasmid (pEGFP-N1) encoding green fluorescent protein (GFP) into human breast adenocarcinoma cells (MCF-7). The cell membrane integrity and viability were fully preserved in this laser-assisted transfer.
Mohanty
Samarendra K
SK
Biomedical Applications Section Center for Advanced Technology, Indore-452013, India.
Sharma
Mrinalini
M
Gupta
Pradeep K
PK
D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002463:Cell Membrane Permeability / Q000528:radiation effects*; D002470:Cell Survival / Q000528:radiation effects; D016503:Drug Delivery Systems / Q000295:instrumentation / Q000379:methods; D049452:Green Fluorescent Proteins; D006801:Humans; D007834:Lasers; D008164:Luminescent Proteins; D008845:Microinjections / Q000295:instrumentation / Q000379:methods*; D010957:Plasmids / Q000008:administration & dosage* / Q000493:pharmacokinetics; D011419:Propidium / Q000008:administration & dosage / Q000493:pharmacokinetics; D011744:Pyrimidinones / Q000008:administration & dosage / Q000493:pharmacokinetics; D014162:Transfection / Q000295:instrumentation / Q000379:methods*; D014170:Transformation, Genetic; D014407:Tumor Cells, Cultured
D008164:Luminescent Proteins; D011744:Pyrimidinones; D049452:Green Fluorescent Proteins; D011419:Propidium; C003954:merocyanine dye