title: Covalent attachment of gold nanoparticles to DNA templates.
abstract: Functionalized gold nanoparticles have been covalently bound to internal, modified sites on double-stranded DNA. Gold nanoparticles coated with mercaptosuccinic acid or thioctic acid were bound to amino-modified thymine bases on double-stranded DNA. Visible absorption spectra, gel electrophoresis, and atomic force microscopy were used to analyze the products. Thiol groups were added to one end of the gold/nanoparticle product, which was then attached to a gold surface. This method has the potential to allow controlled placement of particles with subnanometer precision and to allow attachment of the product to fixed contacts for nanodevice fabrication.
authors.affiliation: Center for Engineering Science Advanced Research, Life Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, USA.
authors.forename: Karen A
authors.initials: KA
authors.lastname: Stevenson
authors.forename: G
authors.initials: G
authors.lastname: Muralidharan
authors.forename: Leon
authors.initials: L
authors.lastname: Maya
authors.forename: Jack C
authors.initials: JC
authors.lastname: Wells
authors.forename: Jacob
authors.initials: J
authors.lastname: Barhen
authors.forename: Thomas
authors.initials: T
authors.lastname: Thundat
mesh_terms: D001665:Binding Sites; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel; D006046:Gold / Q000737:chemistry*; D046911:Macromolecular Substances; D018625:Microscopy, Atomic Force; D008863:Microspheres; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D013054:Spectrophotometry, Atomic; D013194:Staining and Labeling / Q000379:methods*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013499:Surface Properties; D013698:Templates, Genetic; D013936:Thymidine / Q000737:chemistry
chemical_list: D046911:Macromolecular Substances; D013438:Sulfhydryl Compounds; D006046:Gold; D004247:DNA; D013936:Thymidine