Cloning and analysis of the genomic DNA sequence of augmenter of liver regeneration from rat.
OBJECTIVE
To search for genomic DNA sequence of the augmenter of liver regeneration (ALR) of rat.


METHODS
Polymerase chain reaction (PCR) with specific primers was used to amplify the sequence from the rat genome.


RESULTS
A piece of genomic DNA sequence and a piece of pseudogene of rat ALR were identified. The lengths of the gene and pseudogene are 1508 bp and 442 bp, respectively. The ALR gene of rat includes 3 exons and 2 introns. The 442 bp DNA sequence may represent a pseudogene or a ALR-related peptide. Predicted amino acid sequence analysis showed that there were 14 different amino acid residues between the gene and pseudogene. ALR-related peptide is 84 amino acid residues in length and relates closely to ALR protein.


CONCLUSIONS
There might be a multigene family of ALR in rat.
Dong
Jing
J
Gene Therapy Research Center, Institute of Infectious Diseases, the 302 Hospital of PLA, Beijing 100039.
Cheng
Jun
J
Wang
Qinhuan
Q
Shi
Shuangshuang
S
Wang
Gang
G
Si
Chongwen
C
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D005260:Female; D006801:Humans; D008115:Liver Regeneration / Q000235:genetics*; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000235:genetics*; D011544:Pseudogenes / Q000235:genetics; D051381:Rats; D050862:Oxidoreductases Acting on Sulfur Group Donors
D004247:DNA; D011506:Proteins; C088825:GFER protein, rat; D050862:Oxidoreductases Acting on Sulfur Group Donors