[Use of impedance methods for testing bactericidal activity of antiseptics].
Standards PN-EN 1040 and EN 12054 describe test methods and minimum requirements for bactericidal activities of antiseptics. However, standard procedures are time consuming and require 48 hours of incubation. New alternative technique based on impedimetric procedure provides possibilities to reduce this time to several hours. The Bactometer (BioMerieux, Vitek System, USA) is a fully automated impedance technology system used to microbial quantitation of products. Impedance measures microbial activity by electrical methods. The aim of the study was to adaptate the impedimetric method utilising Bactometer--system to microbiological activity control of chemical antiseptics. Eight different products were utilised throughout the study. The samples for classical and alternative method were prepared in the same way as described in standards. The method of choice was dilution-neutralization method. All procedures conducted in Bactometer were verified by plate count method. The high correlation was observed between results obtained by normative methods and impedimetric measurement. All tested products meet requirements. The procedure utilising the Bactometer, provides a rapid and accurate system for the determination of bacterial content. The results of validation carried out during this study indicate, possibility to use impedimetric method alternatively to traditional methods.
Zareba
Tomasz
T
Zakład Antybiotyków i Mikrobiologii Narodowego Instytutu Zdrowia Publicznego w Warszawie.
Bocian
Ewa
E
Tyski
Stefan
S
D000891:Anti-Infective Agents, Local / Q000494:pharmacology* / Q000592:standards*; D017097:Electric Impedance; D008828:Microbiological Techniques / Q000379:methods
D000891:Anti-Infective Agents, Local