title: Analysis of optically active compounds using conventional chromatography with a circular dichroism detector.
abstract: Analysis of optically active compounds in complex samples is often based on chiral chromatography or capillary electrophoresis in order to separate the enantiomers. This requires a chiral reagent, when using conventional chromatography, or an expensive chiral column, or a chiral selector, when using capillary electrophoresis. The type of column, reagent, or additive depends highly on the compound to be analysed. A simple and generally applicable method is using a conventional HPLC column coupled to a CD detector. Separation of enantiomers is not required, as they can be identified by a positive or negative peak. A racemate does not produce a peak; neither does an optically inactive compound. The application of HPLC-CD for the identification of pharmacologically active compounds, such as dexamphetamine, 5-hydroxytryptophan, (-)-huperzine A, and interferon, as standards, in registered drugs, in falsifications, and in food supplements is described.
authors.affiliation: KCF-Centre for Quality of Chemical-Pharmaceutical Products, RIVM-National Institute for Public Health and the Environment, P.O. Box 1, 3720 BA Bilthoven, The Netherlands.
authors.forename: C
authors.initials: C
authors.lastname: Slijkhuis
authors.forename: K D
authors.initials: KD
authors.lastname: Hartog
authors.forename: C
authors.initials: C
authors.lastname: van Alphen
authors.forename: L
authors.initials: L
authors.lastname: Blok-Tip
authors.forename: P M J M
authors.initials: PM
authors.lastname: Jongen
authors.forename: D
authors.initials: D
authors.lastname: de Kaste
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002942:Circular Dichroism / Q000379:methods*; D009904:Optical Rotation; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000737:chemistry*
chemical_list: D004364:Pharmaceutical Preparations