title: Extraction of carotenoids from feces, enabling the bioavailability of beta-carotene to be studied in Indonesian children.
abstract: Previously, we have presented a method for quantifying beta-carotene bioavailability based on analysis in serum, following administration of (13)C-labeled beta-carotene. Because stool samples can be collected noninvasively, we have now extended the method to measure the bioavailability based on measurements in feces. An extraction method was developed to enable measurement of concentrations and degree of isotopic enrichment of retinol, retinyl palmitate and carotenoids in feces. Relative bioavailability of beta-carotene from pumpkin (n = 6) was found to be 1.8 times (interval, 0.6, 5.5) greater than that from spinach (n = 8), based on data from feces compared with 1.7 times (interval, 0.9, 3.1) based on data from serum.
authors.affiliation: Division of Human Nutrition, Wageningen University, Wageningen, The Netherlands.
authors.forename: Machteld
authors.initials: M
authors.lastname: Van Lieshout
authors.forename: Clive E
authors.initials: CE
authors.lastname: West
authors.forename: Peter
authors.initials: P
authors.lastname: Van De Bovenkamp
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Yongkai
authors.initials: Y
authors.lastname: Sun
authors.forename: Richard B
authors.initials: RB
authors.lastname: Van Breemen
authors.forename: Dewi Permaesih
authors.initials: DP
authors.lastname: Muhilal
authors.forename: Michiel A
authors.initials: MA
authors.lastname: Verhoeven
authors.forename: Alain F L
authors.initials: AF
authors.lastname: Creemers
authors.forename: Johan
authors.initials: J
authors.lastname: Lugtenburg
mesh_terms: D001682:Biological Availability; D002247:Carbon Isotopes; D002338:Carotenoids / Q000032:analysis* / Q000097:blood; D002648:Child; D005243:Feces / Q000737:chemistry*; D005260:Female; D006801:Humans; D007214:Indonesia; D008297:Male; D014801:Vitamin A / Q000032:analysis / Q000097:blood; D019207:beta Carotene / Q000032:analysis / Q000097:blood / Q000493:pharmacokinetics*
chemical_list: D002247:Carbon Isotopes; D019207:beta Carotene; D014801:Vitamin A; D002338:Carotenoids