title: A novel chitosanase from Bacillus sp. strain K17: gene cloning and expression in Escherichia coli.
abstract: The gene encoding a novel chitosanase from Bacillus sp. strain K17 was cloned and sequenced. The nucleotide sequence of the gene contained an open reading frame corresponded to a protein of 453 amino acids. The deduced amino acid sequence of the K17 chitosanase exhibited the highest homology to those of family 8 glycanases, suggesting that the enzyme belonged to family 8.
authors.affiliation: Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori-ku, Yokohama 226-8501, Japan.
authors.forename: Rie
authors.initials: R
authors.lastname: Yatsunami
authors.forename: Yuri
authors.initials: Y
authors.lastname: Sakihama
authors.forename: Mamie
authors.initials: M
authors.lastname: Suzuki
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Fukazawa
authors.forename: Shinji
authors.initials: S
authors.lastname: Shimizu
authors.forename: Tomoko
authors.initials: T
authors.lastname: Sunami
authors.forename: Kimiko
authors.initials: K
authors.lastname: Endo
authors.forename: Akio
authors.initials: A
authors.lastname: Takénaka
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nakamura
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology*; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D004926:Escherichia coli / Q000201:enzymology; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D017931:DNA Primers; D011994:Recombinant Proteins; D006026:Glycoside Hydrolases; C019868:chitosanase