title: [Cloning of human B lymphocyte activation-related novel gene].
abstract: OBJECTIVE
  To clone the novel activation-related gene of B lymphocyte.
  
  
  METHODS
  The differential display reversal transcription PCR (DDRT-PCR) technique was applied to analyse the expression difference of mRNA between resting and activated B lymphocyte from human tonsil. The positive differential display cDNA fragment identified by Northern-blotting was chosen as probe to filtrate human activated B lymphocyte cDNA library.
  
  
  RESULTS
  Sixty two differential display cDNA fragments (expressed sequence tag, EST) were obtained. Thirty-two of them were mainly expressed in resting B lymphocyte and thirty were expressed in activated cells. Twenty-five were positive ones after identification by Northern blot analysis. A novel cDNA clone was obtained after using EST30 as a probe to filtrate the human activated B cell cDNA library. The whole cDNA clone was 2,048 bp in length and contains a 630 bp open reading frame. The N end of the deduced amino acid sequence was homologous with KAR3 protein which is a member of kinesins superfamily in yeast.
  
  
  CONCLUSIONS
  A novel possible activation-related gene in human B lymphocyte was obtained.
authors.affiliation: National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China. luxingwu@263.net
authors.forename: X W
authors.initials: XW
authors.lastname: Lu
authors.forename: J Y
authors.initials: JY
authors.lastname: Yin
authors.forename: L X
authors.initials: LX
authors.lastname: Cui
mesh_terms: D000595:Amino Acid Sequence; D001402:B-Lymphocytes / Q000276:immunology*; D001483:Base Sequence; D002478:Cells, Cultured; D002648:Child; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D008213:Lymphocyte Activation / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger