[Polymorphism of insulin receptor substrate-1 gene in patients with coronary heart diseases].
OBJECTIVE
To explore the relationship between the polymorphism of insulin receptor substrate-1 (IRS-1) gene and coronary heart diseases in Chinese population.


METHODS
A total of 113 patients with coronary heart diseases and 194 healthy Chengdu residents with less than 82 mmol/L of TG and 6.2 mmol/L of TC in fasting serum testing were selected and compared. The genotype and allele frequency of IRS-1 gene polymorphism was assayed by polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP). The serum lipids were measured by enzymatic kits.


RESULTS
The GG genotype dominated in the IRS-1 gene with frequency of 0.960 and 0.985 for patients and healthy controls respectively. Difference appeared in R allele frequency (0.015 for healthy controls and 0.040 for patients, P = 0.05). Patients with genotype G/R had significantly higher level of serum TC and lower level of plasma glucose than patients with genotype G/G (P < 0.05).


CONCLUSIONS
IRS-1 gene polymorphism is associated with coronary heart disease in Chinese population. The G/R and G/G genotype of IRS-1 gene contributes to the increasing of serum cholesterol and plasma glucose respectively.
Liu
Rui
R
Laboratory of Internal Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Bai
Huai
H
Liu
Yu
Y
Li
Xian
X
Huang
Minghui
M
Deng
Juelin
J
Liu
Bingwen
B
D000368:Aged; D000369:Aged, 80 and over; D003327:Coronary Disease / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D055504:Insulin Receptor Substrate Proteins; D008297:Male; D008875:Middle Aged; D010750:Phosphoproteins / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length
C526292:IRS1 protein, human; D055504:Insulin Receptor Substrate Proteins; D010750:Phosphoproteins