title: Characterization of eight Y-STR loci and haplotypes in a Chinese Han population.
abstract: In this study we analyzed the eight Y-STR loci, DYS443, DYS444, DYS448, DYS453, DYS455, DYS456, DYS457 (DYS437) and DYS458, investigated haplotype distributions of these Y-STR loci in a Chinese Han population, and sequenced alleles of the eight loci for clarifying the structure. Extracted DNA was amplified by PCR and the PCR products were analyzed by non-denaturing horizontal polyacrylamide gel electrophoresis with a discontinuous buffer system. Alleles were sequenced on an ABI 3700 using a Dye Terminator Cycle sequencing kit. DYS443, DYS453, DYS455 and DYS456 were found to be simple repeat systems, while DYS444, DYS448, DYS457 (DYS437) and DYS458 were complex repeat systems. The gene diversities of DYS443, DYS444, DYS448, DYS453, DYS455, DYS456, DYS457 (DYS437) and DYS458 were 0.7742, 0.7671, 0.7453, 0.3545, 0.0549, 0.6988, 0.6148 and 0.8213, respectively. The haplotype diversity for 8 Y-STR loci was 0.9996, and the discrimination capacity was 0.9815. The results indicate that these eight loci are useful Y-linked markers for forensic applications.
authors.affiliation: Institute of Forensic Medicine, Sichuan University, West China University of Medical Sciences, 610041, Chengdu, Sichuan, P.R. China.
authors.forename: Jian Pin
authors.initials: JP
authors.lastname: Tang
authors.forename: Yi Ping
authors.initials: YP
authors.lastname: Hou
authors.forename: Ying Bi
authors.initials: YB
authors.lastname: Li
authors.forename: Jin
authors.initials: J
authors.lastname: Wu
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Hai Jun
authors.initials: HJ
authors.lastname: Zhang
mesh_terms: D000483:Alleles; D044466:Asian People / Q000235:genetics*; D002681:China; D041322:Chromosomes, Human, Y; D005260:Female; D005554:Forensic Medicine / Q000379:methods; D005828:Genetics, Population; D006239:Haplotypes; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D020080:Tandem Repeat Sequences