title: Cold-inducible expression of the cell division cycle gene CDC48 and its promotion of cell proliferation during cold acclimation in zebrafish cells.
abstract: A member of the ATPases associated with diverse cellular activities (AAA) family, the cell division cycle gene CDC48/VCP (valosin-containing protein)/p97, was cloned from zebrafish and found to be a major cold-inducible protein in fish cells. CDC48 mRNA levels increased significantly after reducing the temperature from 30 to 15 degrees C for 25 days. CDC48 protein levels also increased 2.5-fold after 30 days at cold temperatures. When fish cells overexpressing CDC48 were exposed to a temperature of 15 degrees C, cell proliferation was markedly enhanced in comparison with control cells. By contrast, expression of a mutant molecule with a tyrosine-805 to alanine substitution at the C-terminal phosphorylation site inhibited cell proliferation and induced apoptosis at low temperatures. Therefore, CDC48 may promote cell cycling and cell proliferation via C-terminal tyrosine phosphorylation during cold acclimation in fish cells.
authors.affiliation: National Research Institute of Fisheries Science, 2-12-4, Fukuura, Yokohama 236-8648, Japan.
authors.forename: Shintaro
authors.initials: S
authors.lastname: Imamura
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Ojima
authors.forename: Michiaki
authors.initials: M
authors.lastname: Yamashita
mesh_terms: D000064:Acclimatization / Q000235:genetics*; D000251:Adenosine Triphosphatases; D000595:Amino Acid Sequence; D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000502:physiology*; D002455:Cell Division; D003001:Cloning, Molecular; D003080:Cold Temperature; D018076:DNA, Complementary / Q000302:isolation & purification; D005786:Gene Expression Regulation / Q000502:physiology*; D008969:Molecular Sequence Data; D010766:Phosphorylation; D012333:RNA, Messenger / Q000032:analysis; D016415:Sequence Alignment; D014158:Transcription, Genetic; D014162:Transfection; D000074405:Valosin Containing Protein; D015027:Zebrafish
chemical_list: D018797:Cell Cycle Proteins; D018076:DNA, Complementary; D012333:RNA, Messenger; D000251:Adenosine Triphosphatases; D000074405:Valosin Containing Protein