[Effects of collagen I coating on the porous poly-lactide-co-glycolid on adhesion, proliferation, and differentiation of mesenchymal stem cells].
OBJECTIVE
To investigate the effects of collagen I on the adhesion, proliferation, and differentiation of MSCs on PLGA.


METHODS
Collagen I was added onto the surface of pores in pieces of 3-D porous poly-lactide-co-glycolid (PLGA). Bone marrow-derived mesenchymal stem cells (MSCs) were obtained from New Zealand rabbits and were cultured for 3 generations, inoculated into the pores of PLGA pieces with the volume of 0.3 cm x 1.2 cm x 2.0 cm, and then cultured in solution with [(3)H]-thymidine deoxyribose (TdR). PLGA pieces not coated by collagen I were used as controls. The incorporation rate of [(3)H]-TdR was detected 2, 4, 6, and 8 hours, and 7, 14, and 21 days after culture, shown in count per minute (CPM) value, to determine the adhesion and proliferation of the MSCs. RT-POCR was used to examine the expressions of mRNA of the osteoblast markers: osteocalcin (OCN), alkaline phosphatase (ALP), and osteopontin (OPN). Scanning electron microscopy (SEM) was used to observe the morphology of MSCs.


RESULTS
The CPM value since 6 hours after culture between the experimental group and control group began to be significantly different (both P < 0.05) The CPM values 7, 14, and 21 days after culture between the experimental group and control groups (P < 0.05 or P < 0.01). OCN, ALP, and OPN mRNA were expressed in MSCs of the experimental group and only ALP mRNA was weakly expressed in the control group. SEM showed the distribution of spindle and polygonal cells in the pores of the 3-D PLGA pieces and distribution of cylindrical or round cells in the control group.


CONCLUSIONS
Collagen I is effective in promoting the adhesion, proliferation, and differentiation of MSCs on PLGA.
Liu
Gang
G
Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Hu
Yun-yu
YY
Yan
Yong-nian
YN
Xiong
Zhuo
Z
Wang
Zhe
Z
Lu
Rong
R
Bai
Jian-ping
JP
Yang
Jia-ji
JJ
D000818:Animals; D002448:Cell Adhesion; D002454:Cell Differentiation; D002455:Cell Division; D024042:Collagen Type I / Q000494:pharmacology*; D019344:Lactic Acid; D008648:Mesoderm / Q000166:cytology*; D010006:Osteoblasts / Q000166:cytology*; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D011817:Rabbits; D013234:Stem Cells / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods*
D024042:Collagen Type I; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid