Glutaraldehyde-treated bovine pericardium: effects of lyophilization on cytotoxicity and residual aldehydes.
This work assesses the effect of lyophilization on the cytotoxicity and residual aldehyde concentration of glutaraldehyde-treated and lyophilized bovine pericardium (group A), comparing it to conventional glutaraldehyde-treated bovine pericardium (group B). Cytotoxicity was measured by incubating a pericardium sample from each group in saline and assessing the eluant's influence on cellular growth. Residual aldehydes were measured by HPLC. Although both groups' eluants exhibited some cytotoxicity, the eluant from group A was less cytotoxic, with a cytotoxicity index (IC50(%)) of 41%. Group B eluants all had marked cytotoxic effects; cell growth was 24.15% of the negative control at the most dilute eluant concentration (6.25%). The mean residual glutaraldehyde level was less in group A than in group B (2.36 +/- 0.11 and 9.90 +/- 3.70 g/l, respectively; n=3, P < 0.05), but residual formaldehyde levels did not differ. These results demonstrate that compared with conventional glutaraldehyde-treated bovine pericardium, lyophilized pericardium is less cytotoxic, with fewer glutaraldehyde residues.
Maizato
Marina J S
MJ
Heart Institute, University of São Paulo Medical School, São Paulo, Brazil.
Higa
Olga Z
OZ
Mathor
Mônica B
MB
Camillo
Maria A P
MA
Spencer
Patric J
PJ
Pitombo
Ronaldo N
RN
Zavaglia
Cecília A C
CA
Leirner
Adolfo A
AA
D000447:Aldehydes / Q000032:analysis*; D000818:Animals; D002417:Cattle; D004350:Drug Residues; D005612:Freeze Drying; D005976:Glutaral / Q000633:toxicity*; D010496:Pericardium / Q000187:drug effects*
D000447:Aldehydes; D005976:Glutaral