Effects of 0.075 Gy x-ray irradiation on the expression of IL-10 and IL-12 in mice.
The objective was to observe the effects of 0.075 Gy low dose radiation (LDR) on the synthesis of IL-10 in splenocytes and the secretion of IL-12 by peritoneal macrophages. Kunming mice were selected and randomly grouped. Northern blot and flow cytometry were adopted to detect the changes of IL-10 at mRNA and protein levels, respectively. Northern blot and ELISA were used, respectively, to examine the changes of IL-12 p35/p40 mRNA and IL-12 p70 protein levels. IL-10 mRNA decreased significantly in splenocytes, while both IL-12 p35 and p40 subunit mRNA levels in macrophages increased after whole body irradiation (WBI) with 0.075 Gy x-rays. Meanwhile, IL-10 synthesis in splenocytes decreased beginning from 4 h after exposure and remaining at a lower level up to 48 h, and IL-12 secretion by macrophages was found to take the opposite direction, i.e. increase significantly. In conclusion, WBI with 0.075 Gy x-rays may suppress IL-10 both at the mRNA level and protein level and stimulate IL-12 expression simultaneously, which might contribute to a shift of the immune response in favour of Th1 differentiation.
Liu
Xiao-Dong
XD
Department of Immunology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada. liuxd2002@yahoo.com
Ma
Shu-Mei
SM
Liu
Shu-Zheng
SZ
D000818:Animals; D015152:Blotting, Northern; D002454:Cell Differentiation; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D016753:Interleukin-10 / Q000096:biosynthesis*; D018664:Interleukin-12 / Q000096:biosynthesis*; D017737:Macrophages, Peritoneal / Q000378:metabolism / Q000528:radiation effects*; D008297:Male; D051379:Mice; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000378:metabolism / Q000528:radiation effects*; D018417:Th1 Cells; D013997:Time Factors; D014965:X-Rays
D012333:RNA, Messenger; D016753:Interleukin-10; D018664:Interleukin-12; D012313:RNA