title: Formation and stability of alternate-stranded triplex formed with alpha,beta-chimeric oligoDNA.
abstract: Chimeric oligoDNAs composed of alpha-anomeric oligonucleotide and beta-anomeric oligonucleotide with modified nucleobases and/or phosphodiester backbone are synthesized and their ability to form alternate-stranded triple helix with dsDNA was examined. Substitution of normal phosphodiester linkages with phosphorothioate linkages resulted in the prominent destabilization of the alternate-stranded triplex. Meanwhile, the introduction of cationic molecule or intercalative molecule at certain positions of the modified DNA was effective to increase the stability of the triplexes.
authors.affiliation: Department of Chemistry, Faculty of Engineering, Gunma University, Kiryu 376-8515, Japan.
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Hirose
authors.forename: Aimi
authors.initials: A
authors.lastname: Kadoya
authors.forename: Noritake
authors.initials: N
authors.lastname: Matsumoto
authors.forename: Takanori
authors.initials: T
authors.lastname: Miyashita
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Moriguchi
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shinozuka
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D009690:Nucleic Acid Conformation
chemical_list: D004247:DNA