[Ethanol sensitivity of rat brain cortex Na(+), K(+)-ATPase in plasma membrane structural damage induced by sodium dodecyl sulfate].
To investigate the role of rat brain cortex Na+, K(+)-ATPase plasma membrane microenvironment in ethanol effect in vitro on membrane the sensitivity of enzyme activity to alcohol was studied under membrane perturbation induced by sodium dodecyl sulfate. The increase of enzyme sensitivity to detergent inactivation in the presence of high ethanol concentrations and to alcohol inhibition after modification by Ds-Na was revealed. It is supposed that Na+, K(+)-ATPase sensitivity to ethanol is dependent on structural state of protein microenvironment in accordance with assumed differences in structural organization of the boundary lipids of the neuronal enzyme isoforms.
Mishchuk
D O
DO
Palladin Institute of Biochemistry, NAS of Ukraine, Kyiv.
Zimina
V P
VP
Kaplia
A A
AA
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects* / Q000201:enzymology*; D002540:Cerebral Cortex / Q000187:drug effects / Q000201:enzymology*; D003902:Detergents / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D000431:Ethanol / Q000633:toxicity*; D007527:Isoenzymes / Q000187:drug effects / Q000378:metabolism; D008861:Microsomes / Q000187:drug effects / Q000201:enzymology; D009474:Neurons / Q000187:drug effects / Q000201:enzymology; D051381:Rats; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D000254:Sodium-Potassium-Exchanging ATPase / Q000187:drug effects* / Q000378:metabolism
D003902:Detergents; D004791:Enzyme Inhibitors; D007527:Isoenzymes; D012967:Sodium Dodecyl Sulfate; D000431:Ethanol; D000254:Sodium-Potassium-Exchanging ATPase