title: [Fudenine, relative to blood glucose-control, is a novel membrane protein].
abstract: OBJECTIVE
  To determine whether Fudenine is a novel membrane protein.
  
  
  METHODS
  Green fluorescence protein(GFP) was used to localize Fudenine in vivo. GFP, as a control, was targeted to cytoplasm. Epithelial cell, CBRH7919, and non-epithelial cell, L-6TG, were cultured and transiently transfected by using the lipofectamine reagent. After 48 h, intact cells were examined with fluorescence microscope for Fudenine.
  
  
  RESULTS
  Reporter plasmid pEGFP-N1, as a control, was expressed and localized to cytoplasm. But Fudenine, driven by the cytomegalovirus promoterenhancer contained in the pEGFP-N1 vector, was overexpressed and targeted to cellular membrane.
  
  
  CONCLUSIONS
  Fudenine is a novel membrane protein. It may play the similar role with its homologues AC133 antigen and prominin in human and mouse, respectively. It might be involved in signaling transduction and regulate blood glucose metabolism in vivo.
authors.affiliation: National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China.
authors.forename: Y S
authors.initials: YS
authors.lastname: Chang
authors.forename: J
authors.initials: J
authors.lastname: Zuo
authors.forename: X F
authors.initials: XF
authors.lastname: Zhang
authors.forename: M
authors.initials: M
authors.lastname: Zhang
authors.forename: F D
authors.initials: FD
authors.lastname: Fang
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD; D002460:Cell Line; D006023:Glycoproteins; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000096:biosynthesis* / Q000235:genetics; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D010455:Peptides; D010957:Plasmids / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics; D014162:Transfection
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; D008164:Luminescent Proteins; D008562:Membrane Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; C000606024:Prom1 protein, mouse; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins