Cytokine responses in mice infected with Clonorchis sinensis.
FVB and BALB/c mice show different morbidity, development of Clonorchis sinensis, and pathological changes following C. sinensis infection. FVB mice are susceptible and BALB/c mice are relatively more resistant to C. sinensis infection. To investigate the relationship between cytokine reaction and susceptibility to C. sinensis infection in FVB and BALB/c mice, we described both the patterns and kinetics of Th1 cytokines and Th2 cytokines in spleen cell culture. Interleukin (IL)-4 and IL-10 cytokine production in the culture supernatants of the concanavalin-A-stimulated spleen cells increased at 2-3 weeks post-infection in both strains. IL-5 production increased between 2 and 5 weeks post-infection in both strains, and reached a peak level at 2 weeks post-infection in BALB/c mice and 4 weeks post-infection in FVB mice. In contrast, gamma interferon (IFN-gamma) production decreased between 2 and 4 weeks in both strains. IL-2 production increased slightly in BALB/c mice following infection, but was unchanged in FVB mice. IL-4 production over preinfection levels was significantly higher in FVB mice, whereas IFN-gamma, IL-2, and IL-10 production were significantly higher in BALB/c mice. The levels of serum immunoglobulin E (IgE) and blood eosinophils in both mouse strains significantly increased between 3 and 6 weeks postinfection. Serum IgE levels were significantly higher in FVB mice than in BALB/c mice. The results of this study suggest that susceptibility to C. sinensis infection is associated with Th2 cytokine production, especially IL-4 which is predominant in relatively susceptible FVB mice.
Choi
Yang Kyu
YK
Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea.
Yoon
Byung Il
BI
Won
Young Suk
YS
Lee
Chul Ho
CH
Hyun
Byung Hwa
BH
Kim
Hyoung Chin
HC
Oh
Goo Taeg
GT
Kim
Dae Yong
DY
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D002478:Cells, Cultured; D003003:Clonorchiasis / Q000276:immunology* / Q000469:parasitology / Q000473:pathology; D003004:Clonorchis sinensis / Q000254:growth & development / Q000276:immunology / Q000472:pathogenicity*; D016207:Cytokines / Q000096:biosynthesis*; D004198:Disease Susceptibility; D004804:Eosinophils / Q000276:immunology; D005260:Female; D007073:Immunoglobulin E / Q000097:blood; D008099:Liver / Q000469:parasitology / Q000473:pathology; D051379:Mice; D013154:Spleen / Q000166:cytology; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
D000909:Antibodies, Helminth; D016207:Cytokines; D007073:Immunoglobulin E