Comparative DNA sequence analysis of wheat and rice genomes.
The use of DNA sequence-based comparative genomics for evolutionary studies and for transferring information from model species to crop species has revolutionized molecular genetics and crop improvement strategies. This study compared 4485 expressed sequence tags (ESTs) that were physically mapped in wheat chromosome bins, to the public rice genome sequence data from 2251 ordered BAC/PAC clones using BLAST. A rice genome view of homologous wheat genome locations based on comparative sequence analysis revealed numerous chromosomal rearrangements that will significantly complicate the use of rice as a model for cross-species transfer of information in nonconserved regions.
Sorrells
Mark E
ME
Department of Plant Breeding, Cornell University, Ithaca, New York 14853, USA. mes12@cornell.edu
La Rota
Mauricio
M
Bermudez-Kandianis
Catherine E
CE
Greene
Robert A
RA
Kantety
Ramesh
R
Munkvold
Jesse D
JD
Miftahudin
Mahmoud
Ahmed
A
Ma
Xuefeng
X
Gustafson
Perry J
PJ
Qi
Lili L
LL
Echalier
Benjamin
B
Gill
Bikram S
BS
Matthews
David E
DE
Lazo
Gerard R
GR
Chao
Shiaoman
S
Anderson
Olin D
OD
Edwards
Hugh
H
Linkiewicz
Anna M
AM
Dubcovsky
Jorge
J
Akhunov
Eduard D
ED
Dvorak
Jan
J
Zhang
Deshui
D
Nguyen
Henry T
HT
Peng
Junhua
J
Lapitan
Nora L V
NL
Gonzalez-Hernandez
Jose L
JL
Anderson
James A
JA
Hossain
Khwaja
K
Kalavacharla
Venu
V
Kianian
Shahryar F
SF
Choi
Dong-Woog
DW
Close
Timothy J
TJ
Dilbirligi
Muharrem
M
Gill
Kulvinder S
KS
Steber
Camille
C
Walker-Simmons
Mary K
MK
McGuire
Patrick E
PE
Qualset
Calvin O
CO
D002874:Chromosome Mapping; D018744:DNA, Plant / Q000032:analysis*; D030541:Databases, Genetic; D020224:Expressed Sequence Tags; D023061:Gene Order / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics; D018745:Genome, Plant; D012275:Oryza / Q000235:genetics*; D006109:Poaceae / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA / Q000379:methods*; D012689:Sequence Homology, Nucleic Acid; D014908:Triticum / Q000235:genetics*
D018744:DNA, Plant