Characteristics of collagenolytic enzymes secreted by deuteromycete fungi Aspergillus flavus.
Collagen induced the synthesis of collagenolytic enzymes in the culture of Aspergillus flavus. Enzyme activity of the culture increased during storage and passage on a medium with this inductor. We developed a method for isolation and purification of collagenolytic enzymes and obtained two electrophoretically homogenous enzyme preparations belonging to neutral thermolabile collagenolytic metalloproteinases.
Sukhosyrova
E A
EA
Research and Training and Methodical Center for Biomedical Technologies VILAR, Moscow.
Nikitina
Z K
ZK
Yakovleva
M B
MB
Veshchikova
E V
EV
Bykov
V A
VA
D000596:Amino Acids / Q000378:metabolism; D001231:Aspergillus flavus / Q000201:enzymology*; D018929:Cell Culture Techniques / Q000379:methods; D002614:Chelating Agents / Q000737:chemistry; D003094:Collagen / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D004492:Edetic Acid / Q000737:chemistry; D010450:Endopeptidases / Q000302:isolation & purification / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism
D000596:Amino Acids; D002614:Chelating Agents; D003470:Culture Media; D013395:Sucrose; D003094:Collagen; D004492:Edetic Acid; D010450:Endopeptidases