title: [Comparison of AP-PCR methods, ribotyping (PCR-ribotyping) and pulsed-field electrophoresis (PFGE) for strains of Clostridium difficile producing toxin B and not producing toxin A].
abstract: In this study were used AP-PCR, PCR-ribotyping and pulsed-field elecrophoresis (PFGE) for comparative study of toxin A-negative/toxin B-posi-tive Clostridium difficile strains with deletion in toxin A gen. We investigated nine unrelated clinical strains, isolated from different units and different time from patients suffering to antibiotic associated diarrhea (AAD). We found that toxin A-negative/toxin B-positive C. difficile strains isolated in Poland belonging to a single genotype A, are being similar to the Japanese strains.
authors.affiliation: Katedra i Zakład Mikrobiologii Lekarskiej AM w Warszawie.
authors.forename: Hanna
authors.initials: H
authors.lastname: Pituch
authors.forename: Alex
authors.initials: A
authors.lastname: van Belkum
authors.forename: Piotr
authors.initials: P
authors.lastname: Obuch-Woszczatyński
authors.forename: Felicja
authors.initials: F
authors.lastname: Meisel-Mikołajczyk
authors.forename: Miroslaw
authors.initials: M
authors.lastname: Łuczak
mesh_terms: D001427:Bacterial Toxins / Q000032:analysis; D016360:Clostridioides difficile / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism; D003967:Diarrhea / Q000382:microbiology; D016521:Electrophoresis, Gel, Pulsed-Field; D004761:Enterocolitis, Pseudomembranous / Q000150:complications; D006801:Humans; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D021521:Ribotyping / Q000379:methods*; D013045:Species Specificity
chemical_list: D001427:Bacterial Toxins