Expression of Class III facilitative glucose transporter genes (GLUT-10 and GLUT-12) in mouse and human adipose tissues.
We have examined whether GLUT-10 and GLUT-12, members of the Class III group of the recently expanded family of facilitative glucose transporters, are expressed in adipose tissues. The mouse GLUT-12 gene, located on chromosome 10, comprises at least five exons and encodes a 622 amino acid protein exhibiting 83% sequence identity and 91% sequence similarity to human GLUT-12. Expression of the GLUT-12 gene was evident in all the major mouse adipose tissue depots (epididymal, perirenal, mesenteric, omental, and subcutaneous white; interscapular brown). The GLUT-10 gene is also expressed in mouse adipose tissues and as with GLUT-12 expression occurred in the mature adipocytes as well as the stromal vascular cells. 3T3-L1 adipocytes express GLUT-10, but not GLUT-12, and expression of GLUT-12 was not induced by insulin or glucose. Both GLUT-10 and GLUT-12 expression was also found in human adipose tissue (subcutaneous and omental) and SGBS adipocytes. It is concluded that white fat expresses a wide range of facilitative glucose transporters.
Wood
I Stuart
IS
Liverpool Centre for Nutritional Genomics, Neuroendocrine and Obesity Biology Unit, Department of Medicine, University of Liverpool, Daulby Street, Liverpool L69 3GA, UK. iswood@liverpool.ac.uk
Hunter
Leif
L
Trayhurn
Paul
P
D016475:3T3 Cells; D000273:Adipose Tissue / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D015870:Gene Expression; D051246:Glucose Transport Proteins, Facilitative; D006801:Humans; D051379:Mice; D009004:Monosaccharide Transport Proteins / Q000235:genetics*
D017931:DNA Primers; D051246:Glucose Transport Proteins, Facilitative; D009004:Monosaccharide Transport Proteins; C430746:SLC2A10 protein, human; C449579:SLC2A12 protein, human