Monitoring and controlling the dissolved oxygen (DO) concentration within the high aspect ratio vessel (HARV).
A probe-type oxygen sensor was developed utilizing a radioluminescent (RL)-based light source and a ruthenium-based sensing chemistry for monitoring the dissolved oxygen (DO) concentration in a modified version of the NASA-designed high aspect ratio vessel (HARV), a batch rotating wall vessel. This sensor provided the means to monitor the DO concentration in the HARV without influencing the flow pattern, thereby retaining the low shear HARV environment conducive to the formation of 3-dimensional cell aggregates. This sensor lost significant signal as a result of exposure to the first three autoclave cycles, but only minimal change in signal was observed following exposure to subsequent autoclave cycles. A new calibration model requiring only one fitted parameter was developed that accurately fit data over the entire range from 0% to 100% oxygen saturation. The ability for DO concentration control within the vessel was demonstrated by using this sensor to monitor the DO concentration inside the HARV.
Saarinen
Mark A
MA
Department of Chemical and Biochemical Engineering and Chemistry, University of Iowa, Iowa City, Iowa 52242, USA.
Reece
Julie S
JS
Arnold
Mark A
MA
Murhammer
David W
DW
D000818:Animals; D019149:Bioreactors / Q000382:microbiology*; D002138:Calibration; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002478:Cells, Cultured; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005246:Feedback / Q000502:physiology; D010100:Oxygen / Q000032:analysis* / Q000378:metabolism*; D010101:Oxygen Consumption / Q000502:physiology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods; D018411:Spodoptera / Q000254:growth & development / Q000378:metabolism*; D014159:Transducers
D010100:Oxygen