title: Effects of non-covalent interactions with 5-O-caffeoylquinic acid (chlorogenic acid) on the heat denaturation and solubility of globular proteins.
abstract: The non-covalent interactions between the monomeric phenolic compound chlorogenic acid (5-CQA) and bovine serum albumin (BSA), lysozyme, and alpha-lactalbumin were characterized, and their effect on protein properties was examined. 5-CQA had a low affinity for all three proteins, and these interactions seemed to show a negative cooperativity. 5-CQA-BSA binding decreased with increasing temperature, whereas pH (pH 3.0 compared to pH 7.0) and ionic strength had no pronounced effect. At high 5-CQA/protein molar ratios, both the denaturation enthalpy and temperature of BSA increased; however, covalent bonds were created at high temperatures. The presence of 5-CQA had no effect on the solubility of BSA and alpha-lactalbumin as a function of pH, whereas it decreased lysozyme solubility at alkaline pH due to covalent interactions. These results indicate that the non-covalent interactions with 5-CQA do not have pronounced effects on the functional properties of globular proteins in food systems.
authors.affiliation: Center for Protein Technology, TNO-WU, Wageningen, The Netherlands.
authors.forename: Stéphanie V E
authors.initials: SV
authors.lastname: Prigent
authors.forename: Harry
authors.initials: H
authors.lastname: Gruppen
authors.forename: Antonie J W G
authors.initials: AJ
authors.lastname: Visser
authors.forename: Gerrit A
authors.initials: GA
authors.lastname: Van Koningsveld
authors.forename: Govardus A H
authors.initials: GA
authors.lastname: De Jong
authors.forename: Alfons G J
authors.initials: AG
authors.lastname: Voragen
mesh_terms: D002726:Chlorogenic Acid / Q000737:chemistry*; D004347:Drug Interactions; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007768:Lactalbumin / Q000737:chemistry*; D009113:Muramidase / Q000737:chemistry*; D009994:Osmolar Concentration; D011489:Protein Denaturation; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012995:Solubility; D013816:Thermodynamics
chemical_list: D012710:Serum Albumin, Bovine; D002726:Chlorogenic Acid; D007768:Lactalbumin; D009113:Muramidase