title: DNA lesion and mutagenesis induced in phageM13mp2 by UVA, UVB and UVC irradiation.
abstract: Sunlight is carcinogenic and mutagenic and its genotoxic effects are believed to be the result of UV light-induced lesions in DNA. These lesions include pyrimidine dimers and (6-4) photoproducts, but it is uncertain whether the pyrimidine modifications are the sole pre-mutagenic lesions induced by UV light. Previous studies indicate that some sunlight-induced mutations in the single-stranded DNA phage M13mp2 may not be caused by these photoproducts. In this work, purified single-stranded phage DNA was exposed to UVA, UVB and UVC and the induced mutations were analyzed. All 3 types of UV light increase the mutation frequency. The mutants were sequenced and the results suggest that UVA exposure may induce formation of a non-dipyrimidine lesion in DNA.
authors.affiliation: Faculty of Pharmaceutical Sciences, Gene Research Center, Okayama University, Tsushima, Okayama 700-8530, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Izumizawa
authors.forename: S J
authors.initials: SJ
authors.lastname: Yang
authors.forename: T
authors.initials: T
authors.lastname: Negishi
authors.forename: K
authors.initials: K
authors.lastname: Negishi
mesh_terms: D017104:Bacteriophage M13 / Q000235:genetics* / Q000528:radiation effects*; D004249:DNA Damage; D004252:DNA Mutational Analysis; D004279:DNA, Viral / Q000235:genetics* / Q000528:radiation effects*; D016296:Mutagenesis; D014466:Ultraviolet Rays
chemical_list: D004279:DNA, Viral