Multifunctional microcapsules for pancreatic islet cell entrapment: design, preparation and in vitro characterization.
Great advances in cell transplantation have been made, including the recent, remarkable success in pancreatic islet transplantation for the treatment of type 1 diabetes mellitus. Unfortunately, the transplanted cells are very susceptible to oxidative stress that cause severe damage to either allo- or xenogeneic islets upon graft in diabetic patients. Consequently, the transplanted islet functional life span is significantly shortened. The aim of this study was to examine the possible effects of antioxidants on in vitro cultured adult rat islets, and to evaluate the effects of a prolonged-release formulation, in form of cellulose acetate (CA) microspheres, on Vitamin D(3) activity. Isolated rat islets, both free and entrapped in microspheres were treated with Vitamin D(3). The effects of the vitamin were studied at 3, 6 and 9 days of in vitro cell culture. According to insulin secretory patterns, treatment with Vitamin D(3) of both free and CA entrapped microspheres, increased the insulin output as compared to untreated controls. Such positive effects were confirmed under islet static incubation with glucose at day 6. These results suggest that pancreatic islets can be advantageously treated with anti-oxidising vitamins before implantation, and speculatively, with the help of special delivery systems, throughout the islet cell life span, in the post-transplant time period.
Luca
Giovanni
G
Department of Medicinal Chemistry and Pharmaceutics, University of Perugia, Perugia, Italy.
Basta
Giuseppe
G
Calafiore
Riccardo
R
Rossi
Carlo
C
Giovagnoli
Stefano
S
Esposito
Elisabetta
E
Nastruzzi
Claudio
C
D000818:Animals; D002478:Cells, Cultured; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D002762:Cholecalciferol / Q000737:chemistry / Q000494:pharmacology*; D020099:Coated Materials, Biocompatible / Q000494:pharmacology; D003692:Delayed-Action Preparations; D004058:Diffusion; D007328:Insulin / Q000096:biosynthesis*; D007515:Islets of Langerhans / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D016381:Islets of Langerhans Transplantation / Q000295:instrumentation* / Q000379:methods*; D008297:Male; D008567:Membranes, Artificial; D008863:Microspheres; D051381:Rats; D017207:Rats, Sprague-Dawley; D014183:Transplantation, Heterologous / Q000295:instrumentation / Q000379:methods
D020099:Coated Materials, Biocompatible; D003692:Delayed-Action Preparations; D007328:Insulin; D008567:Membranes, Artificial; D002762:Cholecalciferol; C005062:acetylcellulose; D002482:Cellulose