Study of specific IgG subclass antibodies for diagnosis of Gnathostoma spinigerum.
Gnathostoma spinigerum infection is endemic in Thailand and many Asian countries. Current diagnosis is the skin test and enzyme-linked immunosorbent assay (ELISA) for IgG antibody against the G. spinigerum third-stage larvae (L3), but cross-reactivity is common. We evaluated the sensitivity and specificity of anti-G. spinigerum L3 IgG subclass antibodies for diagnosis of 43 patients with gnathostomiasis. The majority of patients with gnathostomiasis (91%) had eosinophilia. While the anti-G. spinigerum L3 IgG1 antibody provided the highest sensitivity (98%), the anti-G. spinigerum L3 IgG2 antibody had the highest specificity (88%). The ELISA that detected anti-G. spinigerum L3 IgG1 antibody could be a reliable laboratory screening test, while anti-G. spinigerum L3 IgG2 antibody could be used to confirm the diagnosis.
Nuchprayoon
Surang
S
Department of Parasitology, Faculty of Medicine, Chulalongkorn University, 10330 Bangkok, Thailand. fmedstt@md2.md.chula.ac.th
Sanprasert
Vivornpun
V
Suntravat
Montamas
M
Kraivichian
Kanyarat
K
Saksirisampant
Wilai
W
Nuchprayoon
Issarang
I
D000293:Adolescent; D000328:Adult; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood* / Q000276:immunology; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay; D006039:Gnathostoma / Q000254:growth & development / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood* / Q000145:classification; D007814:Larva / Q000276:immunology; D008875:Middle Aged; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D017205:Spirurida Infections / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology
D000909:Antibodies, Helminth; D007074:Immunoglobulin G