Apoplastic pH and monolignol addition rate effects on lignin formation and cell wall degradability in maize.
Monolignol polymerization rate and apoplastic pH and may influence the formation of lignin and its interactions in cell walls. Primary maize walls were artificially lignified by gradual "end-wise" or rapid "bulk" polymerization of coniferyl alcohol at pH 4 or 5.5. Lignification efficiency was greatest for end-wise polymers at pH 5.5 (90-98%), intermediate for bulk polymers formed at either pH (54-82%), and lowest for end-wise polymers at pH 4 (41-53%). End-wise polymers had about 2.2-fold more ether inter-unit linkages and 70% fewer end-groups than bulk polymers. Low pH enhanced the formation of ether linkages in end-wise but not in bulk polymers. Differences in lignin structure did not influence the enzymatic degradability of cell walls, but lowering apoplastic pH from 5.5 to 4.0 during lignification reduced cell wall degradability by 25%. Further studies indicated this pH-dependent depression in degradability was related to cell wall cross-links formed via lignin quinone methide intermediates.
Grabber
John H
JH
U.S. Dairy Forage Research Center, Agricultural Research Service, U.S. Department of Agriculture, 1925 Linden Drive West, Madison, Wisconsin 53706, USA. jgrabber@wisc.edu
Hatfield
Ronald D
RD
Ralph
John
J
D002473:Cell Wall / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry* / Q000378:metabolism; D011108:Polymers / Q000737:chemistry; D003313:Zea mays / Q000737:chemistry*
D011108:Polymers; D008031:Lignin