Chiral analysis of neurotransmitters using cyclodextrin-modified capillary electrophoresis equipped with microfabricated interdigitated electrodes.
We present cyclodextrin-modified capillary electrophoresis equipped with a microfabricated chip consisting of an array of eight interdigitated microband platinum electrodes (IDs) for simultaneous analysis of three chiral models: epinephrine, norepinephrine and isoproterenol. The IDE chip, positioned very close to the capillary outlet, served as an amplification/detection system. Emerging neurotransmitters at the IDE surface were oxidized at +1.1 V by seven electrodes of the array and then detected by the remaining electrode, poised at +0.0 V. There was an amplification effect on the detecting electrode owing to the recycle between the reduced and oxidized forms of the optical isomers at the electrode surface. The detecting "amplification" current response was governed by the applied potential, the detecting electrode position, the number of adjacent electrodes used for recycling and the distance between the oxidative and reductive electrodes. The six chiral forms of the three neurotransmitters were resolved using 25 mM heptakis(2,6,di-o-methyl)-beta-cyclodextrin with a detection limit of approximately 5 microM. The scheme detected a reduced compound at a reducing potential instead of conventional oxidation detection to alleviate electrode fouling and electroactive interferences. The concurrent oxidation/reduction detection of compounds also facilitated and ascertained peak identification as interfering compounds were unlikely to have the same oxidative/reductive characteristics and mobilities as the analytes of interrogation.
Male
Keith B
KB
Biotechnology Research Institute, National Research Council Canada, Montreal, Quebec, H4P 2R2 Canada.
Luong
John H T
JH
D003505:Cyclodextrins; D004563:Electrochemistry; D004566:Electrodes; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D004837:Epinephrine / Q000032:analysis / Q000302:isolation & purification; D007545:Isoproterenol / Q000032:analysis / Q000302:isolation & purification; D018377:Neurotransmitter Agents / Q000032:analysis*; D009638:Norepinephrine / Q000032:analysis / Q000302:isolation & purification; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013237:Stereoisomerism
D003505:Cyclodextrins; D018377:Neurotransmitter Agents; D007545:Isoproterenol; D009638:Norepinephrine; D004837:Epinephrine