title: Age-related smooth muscle reactivity changes in the rat bladder: an in vitro study.
abstract: An experimental study was conducted to investigate developmental changes of the rat detrusor smooth muscle (DSM) reactivity from newborn to adult period. Urinary bladders were obtained from adult (4 months old), 1-month-old and newborn (4-7 days old) male Sprague-Dawley rats. DSM reactivity of the three groups was evaluated in organ chambers. The newborn DSM strips revealed markedly increased purinoceptor- and cholinoceptor-mediated contractions (ATP, carbachol) with increased maximum response (2.98- and 8.96-fold increase for ATP, 2.90- and 4.22-fold increase for carbachol, when the newborn bladder compared with 1-month-old and adult groups, respectively) and sensitivity (1.65- and 1.29-fold increase for ATP when the newborn bladder compared with the 1-month-old and adult groups, respectively). Additionally the maximum contractile response to KCl in the bladder from the newborn was 1.65- and 8.96-fold increased compared to bladders of the 1-month-old and adult groups, respectively. However, no significant changes in the adrenoceptor-mediated relaxation (isoproterenol) of the rat DSM were observed among the groups. These results indicate that development alters the in vitro responsiveness of rat DSM. The newborn rat bladder gains some of the adult bladder properties within 1 month. These changes are likely to reflect the changing role of parasympathetic regulation in the DSM reactivity during development of the rat bladder.
authors.affiliation: Department of Pediatric Surgery, Kocaeli University Medical School Turkey, PK: 20 Suadiye-34740, Istanbul, Turkey. tugaym@hotmail.com
authors.forename: Melih
authors.initials: M
authors.lastname: Tugay
authors.forename: Fürüzan
authors.initials: F
authors.lastname: Yildiz
authors.forename: Tijen
authors.initials: T
authors.lastname: Utkan
authors.forename: Nejat
authors.initials: N
authors.lastname: Gacar
authors.forename: Güner
authors.initials: G
authors.lastname: Ulak
authors.forename: Faruk
authors.initials: F
authors.lastname: Erden
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000375:Aging / Q000502:physiology*; D000818:Animals; D002217:Carbachol / Q000037:antagonists & inhibitors / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology; D009126:Muscle Relaxation / Q000187:drug effects / Q000502:physiology; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology*; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D001743:Urinary Bladder / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*
chemical_list: D011189:Potassium Chloride; D000255:Adenosine Triphosphate; D002217:Carbachol; D007545:Isoproterenol