title: Substrate recognition by the human MTH1 protein.
abstract: A nucleotide pool sanitizing enzyme, the human MTH1 protein, hydrolyzes 2-hydroxy-dATP, 8-hydroxy-dATP, and 8-hydroxyd-GTP. To examine the substrate recognition mechanism of the MTH1 protein, ten nucleotide analogs (8-bromo-dATP, 8-bromod-GTP, deoxyisoinosine triphosphate, 8-hydroxy-dITP, 2-aminopurine-deoxyriboside triphosphate, 2-amino-dATP, deoxyxanthosine triphosphate, deoxyoxanosine triphosphate, dITP, and dUTP) were incubated with the protein. Of these, the former five nucleotides were hydrolyzed with various efficiencies. This results suggests the importance of the anti/syn-conformation and the functional groups on the 2 and 6-positions of the purine ring.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kamiya
authors.forename: Laurence
authors.initials: L
authors.lastname: Dugué
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yakushiji
authors.forename: Sylvie
authors.initials: S
authors.lastname: Pochet
authors.forename: Yusaku
authors.initials: Y
authors.lastname: Nakabeppu
authors.forename: Hideyoshi
authors.initials: H
authors.lastname: Harashima
mesh_terms: D045643:DNA Repair Enzymes; D006801:Humans; D009711:Nucleotides / Q000378:metabolism*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D009711:Nucleotides; D010744:Phosphoric Monoester Hydrolases; C078207:8-oxodGTPase; D045643:DNA Repair Enzymes