Synthesis of non-natural DNA using DNA polymerase.
Nonnatural nucleotide modified by glucose or galactose was synthesized to increase functional diversity of DNA library. These compounds were incorporated in a DNA double strand using Klenow Fragment as well as dTTP. These functional group could be ordered sequentially on a DNA double strand at intervals of few angstroms according to the designed template sequence within a few hours. This method must be useful to constructing nonnatural DNA library or designed supramolecular structures.
Ebara
Y
Y
Division of Natural Environment and Bioorganic Chemistry, Faculty of Human Development and Sciences, Graduate School of Science and Technology, Kobe University, 3-11 Tsurukabuto, Nada-ku, Kobe 657-8501, Japan.
Sugiyama
T
T
Kaihatsu
K
K
Ueji
S
S
D001483:Base Sequence; D004247:DNA / Q000096:biosynthesis* / Q000737:chemistry*; D004256:DNA Polymerase I / Q000378:metabolism*; D003856:Deoxyuracil Nucleotides / Q000737:chemistry / Q000378:metabolism; D015195:Drug Design; D005690:Galactose / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D008958:Models, Molecular; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D013379:Substrate Specificity; D013942:Thymine Nucleotides / Q000737:chemistry / Q000378:metabolism
D003856:Deoxyuracil Nucleotides; D013942:Thymine Nucleotides; C027078:deoxyuridine triphosphate; D004247:DNA; D004256:DNA Polymerase I; D005947:Glucose; C024157:thymidine 5'-triphosphate; D005690:Galactose