Possible relationship of PFGE patterns of Moraxella catarrhalis between hospital- and community-acquired respiratory infections in a community hospital.
We describe a prospective study of molecular analysis of Moraxella catarrhalis isolated from a community hospital. Our study was designed to investigate the possible relationship of pulsed-field gel electrophoresis (PFGE) patterns of M. catarrhalis between hospital- and community-acquired respiratory infections. A nosocomial outbreak of M. catarrhalis was observed between September 2000 and September 2001. During the study period, 40 strains of M. catarrhalis were isolated from a total of 32 patients with respiratory infections (26 strains from 18 inpatients, and 14 strains from 14 outpatients). We compared the PFGE patterns in 40 strains of M. catarrhalis isolated from the respiratory tract of the study patients. The genomic types of M. catarrhalis were classified into three PFGE patterns (A, B, and C). Interestingly, the nosocomial outbreak of M. catarrhalis included two patterns (A and B). Of the three patterns, two patterns (A and B) were found in both inpatients and outpatients. More interestingly, two subtypes of pattern B (B1 and B4) were simultaneously found in both inpatients and outpatients. Our results indicated that PFGE with SmaI chromosomal digestion is a suitable technique to establish the inter-strain genetic relatedness of M. catarrhalis, and suggested that the outbreak of M. catarrhalis occasionally included miscellaneous PFGE patterns. The results also showed that PFGE patterns of M. catarrhalis isolates were similar between hospital- and community-acquired respiratory infections. Analysis of the subtypes suggested that there might be some association between hospital- and community-acquired respiratory infections caused by M. catarrhalis.
Masaki
Hironori
H
Department of Internal Medicine, Tagami Hospital, Nagasaki, Nagasaki 851-0251, Japan. ig7y-mkmt@asahi-net.or.jp
Asoh
Norichika
N
Kawazoe
Kaneo
K
Watanabe
Kiwao
K
Onizuka
Tomoko
T
Shimogama
Seiji
S
Yamaryo
Takeshi
T
Watanabe
Hiroshi
H
Oishi
Kazunori
K
Nagatake
Tsuyoshi
T
D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D017714:Community-Acquired Infections / Q000453:epidemiology / Q000382:microbiology*; D015897:Comorbidity; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis*; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D016905:Gram-Negative Bacterial Infections / Q000453:epidemiology / Q000382:microbiology*; D006764:Hospitals, Community; D006801:Humans; D007297:Inpatients; D007564:Japan / Q000453:epidemiology; D008875:Middle Aged; D001936:Moraxella catarrhalis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010045:Outpatients; D011446:Prospective Studies; D012141:Respiratory Tract Infections / Q000453:epidemiology / Q000382:microbiology*; D015183:Restriction Mapping
D004269:DNA, Bacterial