Evolution of total antioxidant status in a model of acute renal insufficiency in rats.
BACKGROUND
Accurate estimation of the Total Antioxidant Status (TAS) in the myoglobinuric acute renal failure (ARF) is necessary because its pathogenesis is believed to be mediated, at least in part, by the development of oxidative stress resulting from the generation of oxygen free radicals and reduced antioxidant defense system. The purpose of this study is to examine the TAS 24 and 72 h after glycerol injection in a model of myoglobinuric-ARF.


METHODS
The study was conduced in 28 Sprague-Dawley rats. In group 1 (n = 7) rats were placed into individual metabolic cages and deprived of water during 24 h. afterwards an intramuscular injection of glycerol was administrated (50% vol/vol in sterile saline) 10 mg/100 g of body weight and 24 h later blood samples were collected for biochemical measurements (urea, creatinine, creatine-kinase, and TAS levels). In group 2 (n = 7), rats followed the same conditions than group 1 ones but blood samples were collected 72 h after glycerol injection. In groups 3 (n = 7) and 4 (n = 7) rats didn't receive glycerol injection, and blood samples were collected within 24 and 72 h respectively after they were placed into metabolic cages.


RESULTS
In groups 1 and 2 we observed a renal function decrease, with higher serum levels of urea and creatinine in group 2 (urea levels: 269 +/- 16 mg/dL vs. 586 +/- 147 mg/dL; p < 0.001. Creatinine levels: 2.8 +/- 0.2 mg/dL vs. 5.8 +/- 0.7 mg/dL; p < 0.001). TAS levels in groups 2, 3, and 4 were similar, but in group 1 was significantly lower (group 1: 0.81 +/- 0.2 mmol/L; group 2: 1.3 +/- 0.1 mmol/L; group 3: 1.2 +/- 0.3 mmol/L, and group 4: 1.2 +/- 0.2 mmol/L; p < 0.005).


CONCLUSIONS
In the model of glycerol induced myoglobinuric-ARF we observed a decrease of serum TAS level within 24 h with spontaneous recuperation 72 h after.
Fernández-Fúnez
Angel
A
Department of Medicine of General Hospital of Albacete, University of Castilla La Mancha, Albacete, Spain. afernandez@hgab.insalud.es
Polo
Francisco J
FJ
Broseta
Luís
L
Atienza
Maria Paz
MP
Mora
Antonio
A
Gascón
Fernando Garcia
FG
D058186:Acute Kidney Injury / Q000378:metabolism* / Q000503:physiopathology; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood; D003402:Creatine Kinase / Q000097:blood; D003404:Creatinine / Q000097:blood; D004195:Disease Models, Animal; D004231:Diuresis / Q000502:physiology; D007677:Kidney Function Tests; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013223:Statistics as Topic; D014508:Urea / Q000097:blood; D014554:Urination / Q000502:physiology
D000975:Antioxidants; D015415:Biomarkers; D014508:Urea; D003404:Creatinine; D003402:Creatine Kinase