[Recombination and identification of sense and antisense cyclinD1 eukaryotic expression vectors].
The exogenous gene cyclinD1 cDNA was inserted into eukaryotic expression vector pXJ41-neo by gene transduction and recombination techniques. The recombinant was detected by electrophoresis. Results showed that the recombinant sense plasmid (pXJ41-cyclinD1) was digested by HindIII into 229 bp, 427 bp and 7554 bp; while the recombinant antisense plasmid was digested into 427 bp, 1184 bp and 6599 bp, implying that sense and antisense pXJ41-cyclinD1 eukaryotic expression vectors were successfully recombined. The finding from this study offers an effective tool to reveal the mechanism of the carcinogenesis of environmental and occupational hazards.
Yan
Kexia
K
Institute of Occupational Health and Poisons Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Liu
Bingci
B
Xu
Ming
M
You
Baorong
B
Kang
Ning
N
D016375:Antisense Elements (Genetics); D019938:Cyclin D1 / Q000235:genetics*; D005057:Eukaryotic Cells / Q000378:metabolism; D015870:Gene Expression; D005822:Genetic Vectors; D016376:Oligonucleotides, Antisense; D010957:Plasmids / Q000235:genetics; D011995:Recombination, Genetic; D014161:Transduction, Genetic
D016375:Antisense Elements (Genetics); D016376:Oligonucleotides, Antisense; D019938:Cyclin D1