Interaction of peptides derived from RecA with single-stranded oligonucleotides containing 5-formyl-2'-deoxyuridine.
We report the first example of chemical cross-linking of 5-formyl-2'-deoxyuridine containing oligonucleotides with oligopeptides through a Schiff base formation. Twenty amino acid residue peptides investigated here were derived from the DNA binding site of RecA protein. We have demonstrated that the lysine residue placed at the 6th or 8th position from the N-terminus of the peptide directly contacts with DNA.
Sugiyama
T
T
Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Komaba, Meguro-ku, Tokyo 153-8902, Japan.
Kittaka
A
A
Takayama
H
H
Tomioka
M
M
Ida
Y
Y
Kuroda
R
R
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D002942:Circular Dichroism; D003432:Cross-Linking Reagents; D003857:Deoxyuridine / Q000031:analogs & derivatives* / Q000737:chemistry*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D011938:Rec A Recombinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012545:Schiff Bases / Q000737:chemistry
D003432:Cross-Linking Reagents; D029968:Escherichia coli Proteins; D009838:Oligodeoxyribonucleotides; D010455:Peptides; D012545:Schiff Bases; C061519:5-formyl-2'-deoxyuridine; D011938:Rec A Recombinases; D003857:Deoxyuridine