title: Taste receptor T1R3 is an essential molecule for the cellular recognition of the disaccharide trehalose.
abstract: Recently, a sweet taste receptor family, the T1R family, that recognizes some carbohydrates including sucrose was identified. Although the T1R3 molecule is known to participate in heterodimers that are used as sweet- and umami-tasting receptors, there is no evidence that T1R3 alone recognizes similar ligands. We demonstrate for the first time that the candidate sweet taste receptor T1R3 is essential for the recognition and response to the disaccharide trehalose. Our system is a valuable tool not only for understanding the relationship between sweeteners and their receptors but also for exploring the diversities of their receptors, resulting in the design of new high-potency sweeteners.
authors.affiliation: Fujisaki Institute, Hayashibara Biochemical Laboratories, Inc., 675-1 Fujisaki, Okayama 702-8006, Japan. fujisaki@hayashibara.co.jp
authors.forename: Toshio
authors.initials: T
authors.lastname: Ariyasu
authors.forename: Shuji
authors.initials: S
authors.lastname: Matsumoto
authors.forename: Fumiyo
authors.initials: F
authors.lastname: Kyono
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Hanaya
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Arai
authors.forename: Masao
authors.initials: M
authors.lastname: Ikeda
authors.forename: Masashi
authors.initials: M
authors.lastname: Kurimoto
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D006801:Humans; D015759:Ionomycin / Q000378:metabolism; D007476:Ionophores / Q000378:metabolism; D008024:Ligands; D051379:Mice; D008810:Mice, Inbred C57BL; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism; D013549:Sweetening Agents / Q000378:metabolism*; D013649:Taste / Q000502:physiology*; D014199:Trehalose / Q000378:metabolism*
chemical_list: D007476:Ionophores; D008024:Ligands; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D011993:Recombinant Fusion Proteins; D013549:Sweetening Agents; C431926:taste receptors, type 1; D015759:Ionomycin; D013395:Sucrose; D014199:Trehalose; D002118:Calcium