title: 2-Methyl-5-tert-butylthiophenol--an odorless deprotecting reagent useful in synthesis of oligonucleotides and their analogs.
abstract: A solution of 2-methyl-5-tert-butylthiophenol and triethylamine in acetonitrile efficiently removed the methyl protecting groups from phosphate and phosphorothioate triesters and yielded oligonucleotides of high quality. This inexpensive odorless liquid is not toxic and is a suitable replacement for hazardous thiophenol and other reagents often used for this purpose.
authors.affiliation: Isis Pharmaceuticals, Carlsbad, California 92008, USA.
authors.forename: R Krishna
authors.initials: RK
authors.lastname: Kumar
authors.forename: Douglas L
authors.initials: DL
authors.lastname: Cole
authors.forename: Vasulinga T
authors.initials: VT
authors.lastname: Ravikumar
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D005021:Ethylamines / Q000737:chemistry; D009812:Odorants; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D010636:Phenols / Q000737:chemistry*; D013329:Structure-Activity Relationship; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: C482609:2-methyl-5-tert-butylthiophenol; D000097:Acetonitriles; D005021:Ethylamines; D009841:Oligonucleotides; D010636:Phenols; D013438:Sulfhydryl Compounds; C042983:thiophenol; C016162:triethylamine; C032159:acetonitrile