[Effect of compound pattern of ceramic bovine bone and hydrogel on attachment and growth of bone marrow stomal cell].
OBJECTIVE
To investigate the effect of compound pattern of ceramic bovine bone (CBB) and hydrogel(HG) on attachment, proliferation and differentiation of bone marrow stromal cell (MSC), and to find out the best way of constructing tissue engineered bone.


METHODS
CBB, HG and MSC was compounded in different patterns and sequences to form CBB/HG/MSC (group A), HG/MSC/CBB (group B), CBB/MSC/HA (group C) and CBB/MSC (control group). Attachment and morphology of MSC were observed by scanning electronic microscope; the proliferation of MSC was evaluated by cell count; alkaline phosphatase(ALP) activity was examined by histochemistry and type I collagen synthesis was examined by immunohistochemistry staining 5 and 10 days later.


RESULTS
In group A, MSC spread better, and ALP activity of group A was significantly higher than that of group B and control group(P < 0.01); but there was no significant difference between group A and group C(P > 0.05). There was no significant difference in type I collagen synthesis between four groups on the 5th day; but mean gray scale of type I collagen in group B was significantly higher than that in the other groups on the 10th day(P < 0.01).


CONCLUSIONS
Different compound patterns of CBB, HG and MSC affect attachment, proliferation, differentiation of MSC. The compound pattern of CBB/HG/MSC is better than the others.
He
Da-wei
DW
Department of Histopathology, Stomatology College, Fourth Military Medical University, Xi'an Shaanxi, P. R. China 710032.
Jin
Yan
Y
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000502:physiology; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002516:Ceramics; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000494:pharmacology*; D006882:Hydroxyapatites / Q000494:pharmacology; D011108:Polymers / Q000494:pharmacology; D023822:Tissue Engineering
D001672:Biocompatible Materials; D006882:Hydroxyapatites; D011108:Polymers; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate