Species pattern and genetic diversity of Trichoderma in a mid-European, primeval floodplain-forest.
We investigated the occurrence and genetic diversity of Trichoderma in the river Danube national park, a primeval, riparian forest area located south-east of Vienna (Austria) which represents one of the last cases of an original European river-floodplain landscape. Forty-six strains were isolated and identified at the species level by analysis of morphological characters, by sequence analysis of their internal transcribed spacer regions 1 and 2 (ITS 1 and 2) of the rDNA cluster and--in some cases--a fragment of the translation elongation factor 1alpha (tef1) gene, and RAPD-analysis. Twenty-one strains were positively identified as T. harzianum, thirteen as T. rossicum, four as T. cerinum, two as T. hamatum, and one each as T. atroviride and T. koningii: four strains yielded two different ITS1 and 2 as well as tef1 sequence types, which were not alignable with any known species. Our studies show that they represent two new taxa of Trichoderma.
Wuczkowski
Michael
M
Austrian Centre of Biological Resources and Applied Mycology, Institute of Applied Microbiology, University of Agricultural Sciences, Nussdorfer Lände 11, A-1190 Wien, Austria.
Druzhinina
Irina
I
Gherbawy
Youssuf
Y
Klug
Brigitte
B
Prillinger
Hansjörg
H
Kubicek
Christian P
CP
D001317:Austria; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D020459:Genes, rRNA; D014644:Genetic Variation; D016533:Mycological Typing Techniques; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D032107:Populus / Q000382:microbiology; D019105:Random Amplified Polymorphic DNA Technique; D032108:Salix / Q000382:microbiology; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014197:Trees / Q000382:microbiology*; D014242:Trichoderma / Q000145:classification / Q000166:cytology / Q000235:genetics* / Q000302:isolation & purification
D021903:DNA, Ribosomal Spacer