Differentiation of bone marrow stromal stem cells into cardiomyocyte-like cells in different mammalian species.
We describe the possibility of obtaining cardiomyocyte-like cell cultures from rat, guinea pig, and human bone marrow stromal stem cells. The content of troponin I-positive cells attains 35-45% of the total number of cells in the cultures and persists at this level for up to 4 months under differentiation conditions. Spontaneous contractions of cardiomyocyte-like cells were observed after the formation of cell monolayer under differentiation conditions.
Shumakov
V I
VI
Institute of Transplantology and Artificial Organs, Ministry of Health of Russian Federation, Moscow.
Onishchenko
N A
NA
Krasheninnikov
M E
ME
Zaidenov
V A
VA
Potapov
I V
IV
Bashkina
L V
LV
Bersenev
A V
AV
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology*; D048429:Cell Size; D002478:Cells, Cultured; D003470:Culture Media; D006168:Guinea Pigs; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000502:physiology*; D006801:Humans; D009200:Myocardial Contraction; D032383:Myocytes, Cardiac / Q000166:cytology / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology*; D019210:Troponin I / Q000378:metabolism
D003470:Culture Media; D019210:Troponin I