title: Mannose receptor contribution to Candida albicans phagocytosis by murine E-clone J774 macrophages.
abstract: Mannoproteins, as the main constituents of the outer layer of yeast cell walls, are able to interact with phagocytic cells in an opsonin-independent manner through the mannose receptor (MR) and to induce yeast ingestion by the professional phagocytes. Moreover, the MR also mediates endocytosis of soluble ligands through clathrin-coated pits. Here, we studied some aspects of the interaction between the MR and Candida albicans using murine E-clone macrophages and the consequences on MR trafficking. Using a pull-down assay involving mixture E-clone macrophage detergent lysate with mannosylated Sepharose beads and glutaraldehyde-fixed, heat-killed (HK) C. albicans, we found that binding of solubilized MR to mannosylated particles occurred with characteristics similar to the receptor's cell-surface mannose-binding activity. We then demonstrated that MR expressed on E-clone macrophages contributed to phagocytosis of unopsonized, HK C. albicans and that yeast phagocytosis induced a decrease in MR endocytic activity without concomitant degradation of the receptor in the time lapse studied.
authors.affiliation: Laboratoire d'Immunologie et de Parasitologie EA 2413, Université Montpellier I, France. UMR CNRS 5539, Université Montpellier II, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Porcaro
authors.forename: Michel
authors.initials: M
authors.lastname: Vidal
authors.forename: Sylvie
authors.initials: S
authors.lastname: Jouvert
authors.forename: Philip D
authors.initials: PD
authors.lastname: Stahl
authors.forename: Jean
authors.initials: J
authors.lastname: Giaimis
mesh_terms: D000818:Animals; D002176:Candida albicans / Q000378:metabolism*; D002460:Cell Line; D002999:Clone Cells; D006358:Hot Temperature; D006801:Humans; D037181:Lectins, C-Type / Q000378:metabolism*; D008262:Macrophage Activation; D008264:Macrophages / Q000502:physiology*; D008351:Mannans / Q000494:pharmacology; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins / Q000378:metabolism*; D051379:Mice; D008863:Microspheres; D009895:Opsonin Proteins / Q000378:metabolism; D010587:Phagocytosis / Q000502:physiology*; D010588:Phagosomes / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012685:Sepharose / Q000737:chemistry; D014168:Transferrin / Q000494:pharmacology
chemical_list: D037181:Lectins, C-Type; D008351:Mannans; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D009895:Opsonin Proteins; D011956:Receptors, Cell Surface; D014168:Transferrin; D012685:Sepharose