title: [Influence of paternal lymphocyte immunization on the selected subpopulations of peripheral blood lymphocytes in women with recurrent spontaneous abortions of unknown etiology].
abstract: The aim of the study was to evaluate the influence of alloimmunization on the composition of PBL subpopulations in women with recurrent spontaneous abortions (RSA) of unknown etiology.
  
  
  METHODS
  25 women with history of 3-6 consecutive RSA were selected for this study. Immunization with paternal lymphocytes, isolated from 100 ml of peripheral blood, was performed two times before conception (4-weeks interval). The following parameters were studied: (%) of peripheral T CD3+, T CD4+, T CD8+, T CD4+/CD45-RO+, T CD4+/CD29+, T CD8+/CD45-RO+, BCD19+ and NK CD16+/CD56+ lymphocytes; (%) of T CD3+ cells with markers of early (CD69+) and late activation (HLA-DR+) and presenting with IL-2 receptors (CD25+); the influence of alloimmunization on the expression of CD69+ receptors on cultured T CD3+ lymphocytes activated by PHA (CD3+/CD69+/FCS).
  
  
  RESULTS
  The (%) of T CD3+ (61.0% vs. 68.6%), T CD4+ (37.4% vs. 45.3%) cells as well as T CD4+/T CD8+ ratio (1.3 vs. 1.0) significantly increased, whereas the (%) of T CD8+ (41.3% vs. 32.5%) and NK CD16+/CD56+ cells (23.9% vs. 16.2%) decreased after alloimmunization. The procedure didn't influence the (%) of T CD4+/CD45-RO+, T CD4+/CD29+, T CD8+/CD45-RO+ and BCD19+ lymphocytes. We observed significant increase of the (%) of T CD3+/CD69+ (4.4% vs. 7.3%) and T CD3+/CD25+ (5.8% vs. 10.9%) cells after immunotherapy. Moreover we observed the significant increase of the CD69+ expression on T CD3+ cells activated by PHA (CD3+/CD69+/FCS) (42.1 vs. 47.1%).
  
  
  CONCLUSIONS
  Our study suggests that alloimmunization of women with RSA of unknown etiology results in increase of T lymphocytes' activity and a shift of the immune balance towards T helper and T supressor lymphocytes.
authors.affiliation: Kliniki Chirurgii Ginekologicznej ICZMP w Łodzi.
authors.forename: Artur
authors.initials: A
authors.lastname: Szpakowski
authors.forename: Andrzej
authors.initials: A
authors.lastname: Malinowski
authors.forename: Jarosław
authors.initials: J
authors.lastname: Cieślak
authors.forename: Marek
authors.initials: M
authors.lastname: Nowak
authors.forename: Jacek R
authors.initials: JR
authors.lastname: Wilczyński
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Banasik
authors.forename: Marian
authors.initials: M
authors.lastname: Szpakowski
authors.forename: Henryk
authors.initials: H
authors.lastname: Tchórzewski
mesh_terms: D000026:Abortion, Habitual / Q000276:immunology*; D000022:Abortion, Spontaneous / Q000276:immunology; D000328:Adult; D005260:Female; D006801:Humans; D011247:Pregnancy; D018709:Statistics, Nonparametric; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology