title: The expression of NGFr and PGP 9.5 in leprosy reactional cutaneous lesions: an assessment of the nerve fiber status using immunostaining.
abstract: The effects of reactional episodes on the cutaneous nerve fibers of leprosy patients was assessed in six patients (three with reversal reactions and three with erythema nodosum leprosum). Cryosections of cutaneous biopsy of reactional lesions taken during the episode and of another sample during the remission period were immunostained with anti-NGFr and anti-PGP 9.5 (indirect immunofluorescence). We found no significant statistical difference in the number of NGFr- and PGP 9.5-positive fibers between the reactional and post-reactional groups. A significant difference was detected between the number of NGFr and PGP 9.5-stained fibers inside of the reactional group of biopsy cryosections but this difference was ascribed to the distinct aspects of the nerve fibers displayed whether stained with anti-NGFr or with anti-PGP 9.5; NGFr-positive branches looked larger and so interpreted as containing more fibers. In addition, a substantial number NGFr-positive fibers were PGP 9.5-negative. No differences in the number of stained fibers among the distinct cutaneous regions examined (epidermis + upper dermis, mid and deep dermis) was detected. In conclusion, the number of PGP- and NGFr-positive fibers were not significantly different in the reactional and post-reactional biopsies in the present study. NGFr-staining of the nerve fibers is different from their PGP-imunoreactivity and the evaluation of the nerve fiber status on an innervated target organ should be carried out choosing markers for both components of nerve fibers (Schwann cells and axons).
authors.affiliation: Laboratory of Leprosy, Oswaldo Cruz Institute, Rio de Janeiro, RJ, Brazil.
authors.forename: Sérgio Luiz Gomes
authors.initials: SL
authors.lastname: Antunes
authors.forename: Yong
authors.initials: Y
authors.lastname: Liang
authors.forename: José Augusto da Costa
authors.initials: JA
authors.lastname: Neri
authors.forename: Mary
authors.initials: M
authors.lastname: Haak-Frendscho
authors.forename: Olle
authors.initials: O
authors.lastname: Johansson
mesh_terms: D000293:Adolescent; D000328:Adult; D000704:Analysis of Variance; D001369:Axons / Q000737:chemistry; D015415:Biomarkers / Q000032:analysis; D001706:Biopsy; D004893:Erythema Nodosum / Q000378:metabolism* / Q000473:pathology; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D015440:Leprosy, Lepromatous / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009412:Nerve Fibers / Q000737:chemistry*; D020800:Receptor, Nerve Growth Factor / Q000032:analysis*; D012583:Schwann Cells / Q000737:chemistry; D012867:Skin / Q000294:innervation*; D013194:Staining and Labeling / Q000379:methods; D018709:Statistics, Nonparametric; D013869:Thiolester Hydrolases / Q000032:analysis*; D043222:Ubiquitin Thiolesterase
chemical_list: D015415:Biomarkers; D020800:Receptor, Nerve Growth Factor; D013869:Thiolester Hydrolases; D043222:Ubiquitin Thiolesterase