[Purification and characterization of the fibrinolytic enzyme from Agkistrodon halys halys venom].
By Q-sepharose column ion-exchange chromatography, alkyl-sepharose column hydrophobic chromatography the purified fibrinogenolytic enzyme was obtained from Agkistrodon halys halys venom. It is a single peptide-chain with molecular weight about 28 kDa. It was founded that this enzyme cleaved A alpha-chain of fibrinogen, pH-optimum was determined in the range of 7.5-8.0. Its fibrinogenolytic activity was estimated 15.6 mM fibrinogen/min per mg protein; caseinolytic activity was estimated 7.5 c.u., and amidolytic activity was 0.325 mM pNA/min/mg and 0.175 mM pNA/min/mg for S2238 and S2251 respectively; K(m) was 5.6 mM. The enzyme activity was inhibited by DFP and benzamidine. These results suggest that the enzyme is serine protease. It inhibited the platelet-aggregation.
Gornitskaia
O V
OV
Palladin Institute of Biochemistry, NAS of Ukraine, Kyiv.
Rovinskaya
I N
IN
Platonova
T N
TN
D017836:Agkistrodon; D000818:Animals; D001550:Benzamidines / Q000494:pharmacology; D002364:Caseins / Q000378:metabolism; D003435:Crotalid Venoms / Q000201:enzymology* / Q000302:isolation & purification; D005337:Fibrin / Q000378:metabolism; D005340:Fibrinogen / Q000737:chemistry / Q000378:metabolism; D005342:Fibrinolysis; D006863:Hydrogen-Ion Concentration; D007531:Isoflurophate / Q000494:pharmacology; D008970:Molecular Weight; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology; D013379:Substrate Specificity
D001550:Benzamidines; D002364:Caseins; D003435:Crotalid Venoms; D010975:Platelet Aggregation Inhibitors; D011480:Protease Inhibitors; D015842:Serine Proteinase Inhibitors; D007531:Isoflurophate; D005337:Fibrin; D005340:Fibrinogen; C032157:benzamidine