title: [Construction of eucaryotic expression plasmid carrying the BMP7 gene and expression in mesenchymal stem cells].
abstract: OBJECTIVE
  To construct an eucaryotic expression plasmid carrying the BMP7 gene and express in MSCs.
  
  
  METHODS
  The BMP7 gene was cloned into the eucaryotic expression vector pcDNA3.1. At the same time, mesenchymal stem cells (MSCs) were isolated and cultured in vitro. The plasmid carrying the BMP7 gene was transfected into MSCs.
  
  
  RESULTS
  PCR and digesting demonstrated that the eucaryotic expression plasmid -pcDNA-BMP7 was obtained. RT-PCR and immunohistochemical methods showed that the BMP7 gene was expressed in MSCs.
  
  
  CONCLUSIONS
  Construction of an eucaryotic expression plasmid carrying BMP7 gene and expression in MSCs provide a sound basis for gene therapy using the BMP7 gene and the ideal seeds for tissue engineering.
authors.affiliation: Department of Orthopeadics, 304th Hospital, People's Liberation Army, Beijing 100037, China.
authors.forename: Shu-xun
authors.initials: SX
authors.lastname: Hou
authors.forename: Da-ming
authors.initials: DM
authors.lastname: Sun
authors.forename: Gui-xin
authors.initials: GX
authors.lastname: Du
authors.forename: Yi-gang
authors.initials: YG
authors.lastname: Tong
authors.forename: Xiao-bing
authors.initials: XB
authors.lastname: Fu
mesh_terms: D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins / Q000235:genetics*; D015316:Genetic Therapy; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D010957:Plasmids; D016133:Polymerase Chain Reaction; D023822:Tissue Engineering; D016212:Transforming Growth Factor beta
chemical_list: C525819:BMP7 protein, human; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D016212:Transforming Growth Factor beta