title: [Determination of deoxyribonucleic acid with pararosaniline by a resonance light-scattering method].
abstract: The method is based on the enhancement effect of the resonance light-scattering (RLS) of pararosaniline by DNA. In the pH rang of 0.5-1.5, the enhanced intensity of pararosaniline RLS at 355 nm is proportional to the concentration of DNA in the rang of 0.20-15 micrograms.mL-1. The detection limit is 36 ng.mL-1. This method is simple, rapid and has been applied satisfactorily to the determination of DNA in mixed samples.
authors.affiliation: Printed Circuit Board Factory, Zhuzhou Electric Locomotive Institute, Zhuzhou 412001, China.
authors.forename: Hai-yan
authors.initials: HY
authors.lastname: Xiang
authors.forename: Xiao-ming
authors.initials: XM
authors.lastname: Chen
authors.forename: Di-wu
authors.initials: DW
authors.lastname: Zhou
mesh_terms: D000818:Animals; D004247:DNA / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D012394:Rosaniline Dyes; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry / Q000379:methods; D014052:Toluidines
chemical_list: D012394:Rosaniline Dyes; D014052:Toluidines; C005409:pararosaniline; D004247:DNA