title: HPLC analysis of neo-clerodane diterpenoids from Teucrium chamaedrys.
abstract: A simple, rapid analytical method for the quantitative determination of nine neo-clerodane diterpenoids was developed. The neo-clerodane diterpenoids present in the plant material and extracts were separated with an acetonitrile-water gradient at a flow rate of 1 mL per minute. The HPLC separation was performed on a Phenomenex Luna C18(2) (150 x 4.6 mm I.D., particle size 5 microm) reversed phase column with detection at 220 nm. The limit of detection was 0.24-0.90 microg/mL. The relative standard deviation (RSD) values for the determination of neo-clerodane diterpenoids in plant extracts were less than 3.20%. This is the first analytical method developed for qualitative and quantitative analysis of nine neo-clerodane diterpenoids by HPLC with PDA detection.
authors.affiliation: Research Institute of Pharmaceutical Sciences, National Center for Natural Products Research, The University of Mississippi, USA.
authors.forename: B
authors.initials: B
authors.lastname: Avula
authors.forename: R B
authors.initials: RB
authors.lastname: Manyam
authors.forename: E
authors.initials: E
authors.lastname: Bedir
authors.forename: I A
authors.initials: IA
authors.lastname: Khan
mesh_terms: D002138:Calibration; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004224:Diterpenes / Q000032:analysis*; D045785:Diterpenes, Clerodane; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet; D031367:Teucrium / Q000737:chemistry*
chemical_list: D004224:Diterpenes; D045785:Diterpenes, Clerodane