[Protein kinase C in eosinophil proliferation and infiltration in nasal polyp tissue].
OBJECTIVE
To explore the significance of protein kinase C (PKC) in eosinophil (EOS) proliferation and infiltration in nasal polyp tissues.


METHODS
With the methods of in situ hybridization and immunohistochemistry staining, PKC and apoptosis gene (Bcl-2, Bax) expression were measured between nasal polyps from 26 patients and inferior turbinate mucous membrane tissue (ITMMT) from 20 normal persons. EOS was identified with May-Grünwald-Giemsa (MGG) staining.


RESULTS
In EOS, the positive cell expressive rate of Bcl-2 mRNA and its protein were significantly higher in group of nasal polyps than that in group of ITMMT (P < 0.01). Although the positive cell expressive rate of Bax mRNA and its protein were slightly higher in group of nasal polyp tissue than that in group of ITMMT, the difference was not significant (P > 0.05). PKC was expressed in EOS of all 26 cases from nasal polyp tissues, but in 20 cases from ITMMT, only 7 cases showed occasional PKC expression. In both groups, PKC positive cell expression was significantly different, and the expression of PKC and Bcl-2 mRNA as well as their protein in EOS of nasal polyps showed remarkably positive relation (r1 = 0.0875, r2 = 0.0823, P < 0.01).


CONCLUSIONS
PKC expression increasing in EOS of nasal polyp tissues was closely associated with apoptosis inhibiting. So we presumed that EOS inhibiting in nasal polyp tissues was obtained by activating PKC signal transduction pathway.
Li
Mei-hua
MH
Department of Otorhinolaryngology Head and Neck Surgery, China-Japan Union Hospital, Third Clinical Hospital of Jilin University, Changchun 130031, China. meihl@hotmail.com
Dong
Zhen
Z
Yang
Zhan-quan
ZQ
D000328:Adult; D000368:Aged; D017209:Apoptosis; D004804:Eosinophils / Q000201:enzymology* / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000201:enzymology* / Q000473:pathology; D011493:Protein Kinase C / Q000502:physiology*
D011493:Protein Kinase C