Locoregional opening of the rodent blood-brain barrier for paclitaxel using Nd:YAG laser-induced thermo therapy: a new concept of adjuvant glioma therapy?
OBJECTIVE
Nd:YAG laser-induced thermo therapy (LITT) of rat brains is associated with blood-brain barrier (BBB) permeability changes. We address the question of whether LITT-induced locoregional disruption of the BBB could possibly allow a locoregional passage of chemotherapeutic agents into brain tissue to treat malignant glioma.


METHODS
CD Fischer rats were subject to LITT of the left forebrain. Disruption of the BBB was analyzed using Evans blue and immunohistochemistry (IH). Animals were perfused with paclitaxel, and high-pressure liquid chromatography (HPLC) was employed to analyze the content of paclitaxel in brain and plasma samples.


RESULTS
LITT induces an opening of the BBB as demonstrated by locoregional extravasation of Evans blue, C3C, fibrinogen, and IgM. HPLC proved the passage of paclitaxel across the disrupted BBB.


CONCLUSIONS
LITT induces a locoregional passage of chemotherapeutic agents into the brain tissue. This is of potential interest for the treatment of brain tumors.
Sabel
Michael
M
Neurochirurgische Klinik, Heinrich-Heine-Universität, Moorenstrasse 5, D-40225 Düsseldorf, Germany. sabel@uni-duesseldorf.de
Rommel
Frank
F
Kondakci
Mustafa
M
Gorol
Marion
M
Willers
Reinhard
R
Bilzer
Thomas
T
D000287:Administration, Topical; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage*; D001812:Blood-Brain Barrier / Q000503:physiopathology / Q000601:surgery*; D001932:Brain Neoplasms / Q000188:drug therapy*; D017024:Chemotherapy, Adjuvant / Q000379:methods; D005910:Glioma / Q000188:drug therapy*; D006979:Hyperthermia, Induced / Q000379:methods; D053685:Laser Therapy / Q000379:methods*; D019635:Neurosurgical Procedures / Q000379:methods*; D017239:Paclitaxel / Q000008:administration & dosage*; D010539:Permeability / Q000528:radiation effects; D051381:Rats; D011916:Rats, Inbred F344
D000972:Antineoplastic Agents, Phytogenic; D017239:Paclitaxel