[Expression and kinase activity of B-Raf and Raf-1 in leukemia cell lines].
OBJECTIVE
To study expression and kinase activity of B-Raf and Raf-1 kinases in several leukemia cells and normal peripheral blood lymphocytes.


METHODS
Western blot was carried out to detect expression of B-Raf and Raf-1 in peripheral blood lymphocytes and leukemia cells. Kinase activities of Raf-1 and B-Raf were analyzed by immunoprecipitation and Western blot.


RESULTS
The expression levels of Raf-1 were similar in tested leukemia cells and normal peripheral blood lymphocytes, and Raf-1 protein was all minimally tyrosine-phosphorylated. B-Raf expressed in all tested leukemia cells, but not in normal peripheral blood lymphocytes, and B-Raf was highly activated in Jurkat and K562 leukemia cells.


CONCLUSIONS
The abnormal expression and kinase activity of B-Raf in leukemia cells might be related to leukemia etiologically.
Zhang
J
J
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
Liu
Y
Y
Liu
S
S
Zheng
D
D
D006801:Humans; D007938:Leukemia / Q000378:metabolism* / Q000473:pathology; D008214:Lymphocytes / Q000201:enzymology / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism*; D019908:Proto-Oncogene Proteins c-raf / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured
D011494:Protein Kinases; D019908:Proto-Oncogene Proteins c-raf