An effective method for axillary bud culture and RAPD analysis of cloned plants in tetraploid black locust.
An efficient micropropagation technique by axillary bud multiplication was established for cloning tetraploid black locust tree (Robinia pseudoacacia L.). The result showed that the optimal medium for shoot multiplication and elongation was Murashige and Skoog (MS) medium supplemented with 0.5 mg/l 6-benzylaminopurine in combination with 0.5 mg/l kinetin and 0.1 mg/l 1-naphthaleneacetic acid. The best medium for rooting was half-strength MS medium with 0.25 mg/l indole-3-butyric acid. In the present report, we examined the genetic fidelity of the micropropagated plants by the randomly amplified polymorphic DNA (RAPD) method with 25 primers. The cloned plants of tetraploid black locust showed complete stability.
Shu
Q Y
QY
Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 100093 Beijing, China.
Liu
G S
GS
Qi
D M
DM
Chu
C C
CC
Liu
J
J
Li
H J
HJ
D000821:Animal Feed; D002470:Cell Survival; D003001:Cloning, Molecular; D035264:Flowers / Q000235:genetics; D010937:Plant Growth Regulators / Q000494:pharmacology; D011123:Polyploidy; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods; D031301:Robinia / Q000166:cytology / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D014197:Trees / Q000235:genetics
D010937:Plant Growth Regulators