The X-ray crystallographic study of long chain left handed Z-DNA and polyamine complex and structural chemistry study of effection by polyamine for the Z-DNA.
We cleared the stabilization mechanism of Z-DNA by using the molecular dynamics calculation and the grid map simulated annealing docking method. Then, we clarified Z-DNA was stabilized by the metal counter ions whose electrical charge and the number of electrons are too many and by polyamine to go into the minor groove. We succeeded in stabilizing Z-DNA of the long chain by using these methods, and succeeded in the crystallization and the data collection up to 1.7 A resolution.
Ohishi
Hirofumi
H
Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
Suzuki
Kenji
K
Grzeskowiak
Kazimierz
K
Fukuyama
Keiichi
K
Ishida
Toshimasa
T
D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry*; D011073:Polyamines / Q000737:chemistry*
D011073:Polyamines; D004247:DNA