title: Alterations in the erythrocyte antioxidant system of blood stored in blood bags.
abstract: In the present study, we measured the concentrations of reduced glutathione (GSH) and malonyldialdehyde (MDA) and the activities of glutathione peroxidase (GSH-Px), glutathione S-transferase (GSH-S-T), superoxide dismutase (SOD), catalase (CAT) and glucose-6-phosphate dehydrogenase (G-6-PD) in erythrocytes obtained freshly from adult male donors which was preserved with CPDA-1 anticoagulant (citrate,phosphate, dextrose, adenine) on different days of storage. At the end of the study, storage-associated alterations in antioxidant activities were noted and discussed. GSH, GSH-Px, GSH-S-T, SOD, CAT and G-6-PD activities decreased, but erythrocyte MDA levels, as anindex of lipid peroxidation, increased during the storage period. According to our results, glutathione-dependent antioxidant systems in erythrocytes might be depleted during long storage in blood bags.
authors.affiliation: Akdeniz University, Medical Faculty, Department of Biochemistry, Antalya, Turkey.
authors.forename: D K
authors.initials: DK
authors.lastname: Korgun
authors.forename: S
authors.initials: S
authors.lastname: Bilmen
authors.forename: A
authors.initials: A
authors.lastname: Yesilkaya
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000494:pharmacology*; D001793:Blood Preservation; D002374:Catalase / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D013482:Superoxide Dismutase / Q000187:drug effects
chemical_list: D000975:Antioxidants; D008315:Malondialdehyde; D005954:Glucosephosphate Dehydrogenase; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase