title: Differential expression of neuropilin-1 in malignant and benign prostatic stromal tissue.
abstract: Neuropilin-1 (NRP-1), a co-receptor for VEGF165, is overexpressed in various prostate cancer cell lines and in advanced prostate tumors. However, distribution of the NRP-1 in prostate tumors has not yet been evaluated. Using immunohistochemical analysis, we evaluated 21 archival prostate tumors and 5 benign glands for the expression of NRP-1. In addition, we utilized a quantitative RT-PCR method to examine mRNA expression in 9 additional prostate tumors obtained from radical prostatectomy specimens and compared this expression to the adjacent normal tissue. The RT-PCR analyses demonstrated overexpression of NRP-1 mRNA in malignant tissue samples by 10.0-fold as compared to adjacent normal tissue. By immunohistochemistry, NRP-1 protein was undetected or minimally detected in the epithelial tumor cells. However, NRP-1 immuno-reaction was detected in the surrounding tumor stroma. Variable immuno-reaction for NRP-1 was also seen in the adjacent normal tumor stroma and the stroma of the benign prostate samples. These observations suggest that neuropilin-1 is expressed in the prostatic stromal cells, not epithelial tumor cells, and this expression is significantly increased in the malignant phenotype.
authors.affiliation: Cancer Research Unit, V.A. Medical Center, Research Division, Kansas City, MO 64128, USA.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Vanveldhuizen
authors.forename: Muhammad
authors.initials: M
authors.lastname: Zulfiqar
authors.forename: Snigdha
authors.initials: S
authors.lastname: Banerjee
authors.forename: Rachel
authors.initials: R
authors.lastname: Cherian
authors.forename: Neela K
authors.initials: NK
authors.lastname: Saxena
authors.forename: Amy
authors.initials: A
authors.lastname: Rabe
authors.forename: J Brantley
authors.initials: JB
authors.lastname: Thrasher
authors.forename: Sushanta K
authors.initials: SK
authors.lastname: Banerjee
mesh_terms: D000368:Aged; D045744:Cell Line, Tumor; D018076:DNA, Complementary / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D039942:Neuropilin-1 / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism; D010641:Phenotype; D011467:Prostate / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D039942:Neuropilin-1