[Mineralized collagen based composite for bone tissue engineering].
OBJECTIVE
To construct a mineralized collagen based composite by biomimetic synthesis for bone tissue engineering.


METHODS
Using the molecular collagen as the template, the calcium phosphate is deposited on it to produce a mineralized collagen based composite, then is combined with minute amount of poly lactic acid (PLA), the three-dimensional scaffold composite is prepared by liquid phase separation. Using osteoblast culture technique, the biocompatibility of this biomaterial in vitro is detected by x-ray diffraction, SEM, TEM, fluoroscopy and CLSM.


RESULTS
Both degree and the size of crystals in the composite are low, which are similar to that of nature bone. It possesses porous structure and the porosity of the composite is high. The typical fibrillar microstructure is self-assembled of the collagen and the nano-crystal hydroxyapatite (HA) in the composite, moreover, the x-ray diffraction graphic of HA crystal shows the [002]-oriented.


CONCLUSIONS
The biomimetic three-dimensional composite can serve as one of the optimal scaffold material for bone tissue engineering both on structure and on property.
Liao
Su-san
SS
Biomaterials Laboratory, Department of Material Science and Engineering, Tsinghua University, Beijing 100084, China.
Cui
Fu-zhai
FZ
Zhang
Wei
W
D000818:Animals; D000831:Animals, Newborn; D001672:Biocompatible Materials / Q000737:chemistry; D018786:Bone Substitutes; D002130:Calcium Phosphates; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry; D036103:Nanotechnology; D016348:Osseointegration; D010006:Osteoblasts / Q000166:cytology*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D023822:Tissue Engineering
D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D003094:Collagen; C020243:calcium phosphate