The immunostimulatory activity and stability of grass carp (Ctenopharyngodon idellus) roe lectin.
A hexameric rhamnose-specific lectin with a molecular mass of 205kDa and exhibiting some N-terminal sequence similarity to other fish lectins was isolated from roe of the grass carp (Ctenopharyngodon idellus) by affinity chromatography on rhamnose-Sepharose and ion exchange chromatography by fast protein liquid chromatography on a Mono S column. The lectin exhibited mitogenic activity toward murine splenocytes with a potency lower than that of the plant lectin ConA. It exerted a stimulatory effect at a concentration of 10 micro g/ml on the phagocytic activity of seabream (Sparus sarba) macrophages. It was unstable toward heat (temperature > or =40 degrees C), acid (0.1M HCl), alkali (0.1M NaOH), trypsin and succinylation.
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Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China. biochemistry@cuhk.edu.hk
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D000276:Adjuvants, Immunologic / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D000595:Amino Acid Sequence; D000818:Animals; D002347:Carps / Q000276:immunology / Q000378:metabolism*; D002846:Chromatography, Affinity / Q000662:veterinary; D002852:Chromatography, Ion Exchange / Q000662:veterinary; D004355:Drug Stability; D006384:Hemagglutination / Q000276:immunology; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D037102:Lectins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D012210:Rhamnose / Q000378:metabolism*; D021541:Sea Bream; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D014357:Trypsin / Q000378:metabolism
D000276:Adjuvants, Immunologic; D037102:Lectins; D014357:Trypsin; D012210:Rhamnose