[The effects of nm23-H1 on metastases ability and chemo-sensitivity in Tca8113 cell lines].
OBJECTIVE
The purpose of this study was to establish a stable, high-efficient and low-toxic way of transfecting nm23-H1 into Tca8113 line cells, and then to find out whether nm23-H1 could affect the invasion and metastases ability of Tca8113 line cells.


METHODS
nm23-H1 was transfected into Tca8113 line cells with Lipofect. The different expressions of nm23-H1 between transfected and non-transfected line cells were detected by the method of immunohistochemistry. The difference of the invasion and metastases ability between transfected and non-transfected line cells was detected by transwell-room and wash techniques. The change of chemo-sensitivity was evaluated by MTT.


RESULTS
Using pCMV-NEO-BAM system to keep stable expression of nm23-H1, the significant difference of NDPKA expression between transfected and non-transfected Tca8113 line cells was discovered; The metastases ability of transfected Tca8113 line cells decreased significantly; The chemo-sensitivity of transfected Tca8113 line cells to CDDP increased significantly.


CONCLUSIONS
nm23-H1 can inhibit the metastases of Tca8113 line cells and increase the chemo-sensitivity to CDDP significantly.
Chen
Shaowei
S
Department of Oral Maxillofacial Surgery, the Second Affiliated Hospital, Sun Yat-sen University, Guangzhou 510120, China.
Huang
Hongzhang
H
Pan
Jian
J
D000970:Antineoplastic Agents / Q000494:pharmacology; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D002945:Cisplatin / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D054778:NM23 Nucleoside Diphosphate Kinases; D009362:Neoplasm Metastasis; D009701:Nucleoside-Diphosphate Kinase; D011506:Proteins / Q000235:genetics*; D014062:Tongue Neoplasms / Q000235:genetics* / Q000473:pathology; D014162:Transfection; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D054778:NM23 Nucleoside Diphosphate Kinases; D011506:Proteins; C517938:NME1 protein, human; D009701:Nucleoside-Diphosphate Kinase; D002945:Cisplatin