[Correlation analysis between WNK4 gene and essential hypertension].
OBJECTIVE
To investigate association of mutation in WNK4 gene with essential hypertension and to analyze the expression of WNK4 gene.


METHODS
cSNP in WNK4 gene in a small samples was detected by sequencing, then PCR-RFLP was performed in 98 patients with essential hypertension and 95 control subjects. The expression profile of WNK4 gene was tested by RT-PCR.


RESULTS
A cSNP was detected in WNK4 gene exon7 G1662A, and there were significant differences in the distribution of allele frequency of G1662A between essential hypertension group and control group. WNK4 gene were expressed in the tissues of kidney, brain, lung, heart, spleen and intestine of fetus.


CONCLUSIONS
WNK4 gene is well correlated with essential hypertension.
Sun
Zhi-jun
ZJ
Department of Genetics, China Medical University, Shenyang 110001, China.
Wang
Xin-nan
XN
Lu
Jing-yu
JY
Ding
Qian
Q
Dong
Ling-yue
LY
Zhao
Yan-yan
YY
D000328:Adult; D001921:Brain / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D005787:Gene Frequency; D005796:Genes; D020022:Genetic Predisposition to Disease; D006801:Humans; D006973:Hypertension / Q000235:genetics*; D007668:Kidney / Q000378:metabolism; D008239:Lysine / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009154:Mutation; D010641:Phenotype; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
D012333:RNA, Messenger; D017346:Protein Serine-Threonine Kinases; C433806:WNK4 protein, human; D008239:Lysine