title: Polydeoxyribonucleotide (PDRN) promotes human osteoblast proliferation: a new proposal for bone tissue repair.
abstract: Several researchers have recently shed new light upon the importance of extracellular nucleotides and nucleosides to stimulate cells growth. PDRN, a mixture of deoxyribonucleotides polymers of different lengths, has recently demonstrated to stimulate "in vitro" fibroblast proliferation and collagen production, probably stimulating the purinergic receptor system. In this work we evaluated the effects of PDRN on human cultured osteoblasts, focusing our attention on cell proliferation and alkaline phosphatase activity. PDRN at a concentration of 100 microg/ml induce an increase in osteoblasts growth after 6 days as compared to control (+21%). The addition of DMPX 50 microM and suramine (P2 inhibitor) 10 microM give different results: suramine has no significant effect, while DPMX reduce, even if partially, the PDRN induced cell growth. The alkaline phosphatase activity shows a gradual enhancement starting from day 0 to day 10, even if PDRN treated cells, examined at day 6, present a sensibly lower phosphatase activity when compared to controls. Our data demonstrate that PDRN acts as an osteoblast growth stimulator. Its action is partially due to a stimulation of the purinergic system mediated by A2 purinoreceptors, however we can not exclude the involvement of other mechanism like salvage pathway.
authors.affiliation: Department of Experimental Medicine--Section of Histology, Via Volturno 39, University of Parma, 43100 Parma, Italy. stegui@unipr.it
authors.forename: Stefano
authors.initials: S
authors.lastname: Guizzardi
authors.forename: Carlo
authors.initials: C
authors.lastname: Galli
authors.forename: Paolo
authors.initials: P
authors.lastname: Govoni
authors.forename: Renata
authors.initials: R
authors.lastname: Boratto
authors.forename: Giulia
authors.initials: G
authors.lastname: Cattarini
authors.forename: Desiree
authors.initials: D
authors.lastname: Martini
authors.forename: Silvana
authors.initials: S
authors.lastname: Belletti
authors.forename: Renato
authors.initials: R
authors.lastname: Scandroglio
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001861:Bone Regeneration / Q000502:physiology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002675:Child, Preschool; D006801:Humans; D010006:Osteoblasts / Q000187:drug effects* / Q000201:enzymology; D011089:Polydeoxyribonucleotides / Q000494:pharmacology*; D058915:Purinergic P1 Receptor Antagonists; D058919:Purinergic P2 Receptor Antagonists; D013498:Suramin / Q000494:pharmacology; D013805:Theobromine / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D011089:Polydeoxyribonucleotides; D058915:Purinergic P1 Receptor Antagonists; D058919:Purinergic P2 Receptor Antagonists; C057837:3,7-dimethyl-1-propargylxanthine; D013498:Suramin; D000469:Alkaline Phosphatase; D013805:Theobromine