title: Expression and localization of the linear DNA plasmid-encoded protein (RS224) in Rhizoctonia solani AG2-2.
abstract: Expression of the linear DNA plasmid-encoded protein (RS224) from the plant-pathogenic fungus Rhizoctonia solani isolate H-16, anastomosis group 2-2, and its localization were studied. Extracts from Escherichia coli cells expressing the open reading frame (ORF) of RS224 (RS224ORF in pRS224) contain a 92-kDa T7.Tag-RS224orf fusion protein. Antisera raised against the fusion protein obtained from E. coli cells cross-reacted with a 90-kDa protein in the mycelia. To analyze the subcellular localization of the 92-kDa protein, mycelia of R. solani were disrupted and fractionated. Antibodies against RS224 proteins specifically reacted to the mitochondrial fraction, suggesting that RS224 is localized in mitochondria.
authors.affiliation: Department of Environmental Biotechnology, Graduate School of Agriculture, Tohoku University, Sendai 981-8555, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Nagasaka
authors.forename: Atsuko
authors.initials: A
authors.lastname: Sasaki
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Sasaki
authors.forename: Miho
authors.initials: M
authors.lastname: Yonezawa
authors.forename: Koji
authors.initials: K
authors.lastname: Katsura
authors.forename: Teruyoshi
authors.initials: T
authors.lastname: Hashiba
mesh_terms: D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005800:Genes, Fungal; D008928:Mitochondria / Q000378:metabolism; D008970:Molecular Weight; D016366:Open Reading Frames; D010957:Plasmids / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012232:Rhizoctonia / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; D011993:Recombinant Fusion Proteins