Size exclusion chromatography on soft and semi-rigid packing materials in the dynamic axial compression mode.
The effect of dynamic axial compression within a range of up to 5 bar upon the structure of the bed packed with soft and semi-rigid packing materials (Sephadex G-25, Bio-Gel P2 and Toyopearl HW-40) and the associated chromatographic parameters were studied for size exclusion chromatography. Continuous packing compression is accomplished by use of a special column with controlled external pressure applied to the packing. Compression has been shown to favor an overall increase in the resolution with pressure optima observed in some cases.
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MB
Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow Region 142290, Puschino, Russian Federation. synchro@fibkh.serpukhov.su
Danilov
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AV
Vagenina
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D000180:Acrylic Resins / Q000737:chemistry*; D001426:Bacterial Proteins / Q000032:analysis; D002850:Chromatography, Gel / Q000295:instrumentation* / Q000379:methods*; D019245:Compressive Strength; D003911:Dextrans / Q000737:chemistry*; D004548:Elasticity; D019544:Equipment Failure Analysis; D008422:Materials Testing; D016062:Porosity; D011312:Pressure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000180:Acrylic Resins; D001426:Bacterial Proteins; D003911:Dextrans; C016680:polyacrylamide gels; C025614:sephadex