Pseudorabies virus US3 protein kinase mediates actin stress fiber breakdown.
Disruption of specific components of the host cytoskeleton has been reported for several viruses and is thought to be beneficial for viral replication and spread. Our previous work demonstrated that infection of swine kidney (SK-6) cells with pseudorabies virus (PRV), a swine alphaherpesvirus, induced actin stress fiber breakdown. In the present study, using several PRV deletion mutants, we found that the US3 serine/threonine (S/T) protein kinase is involved in breakdown of actin stress fibers in different PRV-infected cell lines. Further, by transfection assays, we showed that PRV US3 itself, in the absence of other viral proteins, is able to trigger actin stress fiber breakdown when it is localized in sufficient amounts in the nucleus.
Van Minnebruggen
Geert
G
Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium.
Favoreel
Herman W
HW
Jacobs
Liesbeth
L
Nauwynck
Hans J
HJ
D000199:Actins / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D011558:Herpesvirus 1, Suid; D006868:Hydrolysis; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D014764:Viral Proteins
D000199:Actins; D014764:Viral Proteins; D017346:Protein Serine-Threonine Kinases; C095207:US3 protein, Human herpesvirus 1