Formation of enterovirus-like particle aggregates by recombinant baculoviruses co-expressing P1 and 3CD in insect cells.
The assembly of enterovirus requires the cleavage of P1 polyprotein by protease 3CD into individual structural proteins. Two recombinant baculoviruses were constructed to encode P1 and 3CD of enterovirus 71 (EV71), respectively. The expressed 3CD successfully cleaved P1 in vitro and in vivo. Also, the co-infection in insect cells resulted in crystalline virus-like particle structures morphologically resembling the authentic EV71 aggregates, which are reported for the first time.
Hu
Yu-Chen
YC
Department of Chemical Engineering, National Tsing Hua University, Hsinchu 300, Taiwan. yuchen@che.nthu.edu.tw
Hsu
John Tsu-An
JT
Huang
Jen-Huang
JH
Ho
Mei-Shang
MS
Ho
Yi-Chen
YC
D000086042:3C Viral Proteases; D000818:Animals; D016367:Baculoviridae / Q000235:genetics / Q000378:metabolism; D036022:Capsid Proteins / Q000096:biosynthesis* / Q000235:genetics; D003546:Cysteine Endopeptidases / Q000096:biosynthesis*; D004770:Enterovirus / Q000235:genetics* / Q000502:physiology; D015967:Gene Expression Regulation, Viral; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D011995:Recombination, Genetic; D018411:Spodoptera / Q000821:virology; D014162:Transfection; D014760:Viral Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D014764:Viral Proteins / Q000096:biosynthesis*; D014771:Virion / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology* / Q000648:ultrastructure; D019065:Virus Assembly / Q000235:genetics / Q000502:physiology*
D036022:Capsid Proteins; D011994:Recombinant Proteins; D014760:Viral Fusion Proteins; D014764:Viral Proteins; D003546:Cysteine Endopeptidases; D000086042:3C Viral Proteases