Effects of leptin gene expression in mice in vivo by electroporation and hydrodynamics-based gene delivery.
In vivo electroporation and hydrodynamics-based gene delivery were utilized to test the effect of leptin gene transfer on food intake, and body and fat weights of mice. Gene transfer of pVRmob by electroporation caused a significant reduction in body weight compared with the control counterpart (p<0.05), although a lesser effect was found in food intake, and the weights of interscapular brown and epididymal fat by electroporation. As might be expected, the hydrodynamics-based transfection method significantly reduced body weight over 1 week post-transfection (p<0.05). Furthermore, epididymal fat was decreased by 50% at 1 week after gene transfer (p<0.001). These results suggest that both electroporation and hydrodynamics-based gene delivery may be effective approaches for systemic delivery of recombinant leptin to the central nervous system, and that the efficiency of gene transfer in hydrodynamics-based gene delivery was markedly higher than that in electroporation at least within the first week after transfection.
Xiang
Lan
L
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan.
Murai
Atsushi
A
Sugahara
Kunio
K
Yasui
Akihiro
A
Muramatsu
Tatsuo
T
D000273:Adipose Tissue / Q000033:anatomy & histology; D000818:Animals; D001835:Body Weight; D004435:Eating; D018274:Electroporation; D015870:Gene Expression; D049452:Green Fluorescent Proteins; D020738:Leptin / Q000096:biosynthesis / Q000097:blood / Q000235:genetics*; D008164:Luminescent Proteins / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D018482:Muscle, Skeletal / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis; D014162:Transfection / Q000379:methods*; D014867:Water / Q000737:chemistry
D020738:Leptin; D008164:Luminescent Proteins; D012333:RNA, Messenger; D014867:Water; D049452:Green Fluorescent Proteins