title: [Factors affecting the determination of the percent carboxyhemoglobin saturation of blood].
abstract: OBJECTIVE
  To investigate factors affecting the determination of the percent carboxyhemoglobin saturation (HbCO%) of blood in an attempt to offer further data for results interpretation and sample storage requirement.
  
  
  METHODS
  The HbCO% of blood samples stored in various conditions were detected by three spectrophotometries during the succeeding 30 days.
  
  
  RESULTS
  The data detected by reductive double-wavelength spectrophotometry and double-wavelength spectrophotometry were more stable than mono-wavelength spectrophotometry. The HbCO% of blood was significantly related with the storage conditions which include temperature, time and the degree of exposure to air.
  
  
  CONCLUSIONS
  Determinations of HbCO% are reliable which performed by reductive double-wavelength spectrophotometry and double-wavelength spectrophotometry, combine with spectral scans. During 30 days, blood stored at 4 degrees C exposed to a limited volume of air does not influence the determination of HbCO%.
authors.affiliation: Department of Forensic Medicine, Medical Center of Fudan University, Shanghai 200032, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jiang
authors.forename: Yong-hong
authors.initials: YH
authors.lastname: Ye
authors.forename: Yi-fan
authors.initials: YF
authors.lastname: Zhang
mesh_terms: D002249:Carbon Monoxide Poisoning / Q000097:blood / Q000175:diagnosis*; D002263:Carboxyhemoglobin / Q000032:analysis*; D005554:Forensic Medicine; D006801:Humans; D013053:Spectrophotometry / Q000379:methods; D013696:Temperature
chemical_list: D002263:Carboxyhemoglobin