Extraction and quantitation of carfentanil and naltrexone in goat plasma with liquid chromatography-mass spectrometry.
This method is the first analytical method for the detection and quantitation of carfentanil and naltrexone at clinically relevant concentrations using liquid chromatography-mass spectrometry. Samples were alkalinized with 100 microl of 1 M NaOH and extracted 2x with 2 ml of toluene. The extractions were combined and dried under N(2) at 40 degrees C in a H(2)O bath. Chromatography was performed using a Zirchrom PBD column and a mobile phase of 30:70 acetonitrile/10 mM ammonium acetate and 0.1 mM citrate (pH=4.4) at a flow rate of 0.3 ml/min. The lower limit of quantitation was 8.5 pg/ml for carfentanil and 0.21 ng/ml for naltrexone.
Hunter
Robert P
RP
Department of Anatomy and Physiology, Kansas State University, 129 Coles Hall, Manhattan, KS 66506-5802, USA. rhunter@vet.ksu.edu
Koch
David E
DE
Mutlow
Adrian
A
Isaza
Ramiro
R
D000701:Analgesics, Opioid / Q000097:blood*; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D005283:Fentanyl / Q000031:analogs & derivatives* / Q000097:blood*; D006041:Goats; D009271:Naltrexone / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D000701:Analgesics, Opioid; D009271:Naltrexone; C017114:carfentanil; D005283:Fentanyl