title: A novel fluorescent nonnatural amino acid that can be incorporated into a specific position of streptavidin.
abstract: A novel highly fluorescent nonnatural amino acid (beta-(N-methylanthraniloyl)-L-alpha,beta-diaminopropionic acid; nmaDap) was incorporated at the 120th position of streptavidin. The position was directed by a CGGG/CCCG four-base codon/anticodon pair. The incorporation efficiency and the biotin-binding ability of the nmaDap mutant were compared to those of beta-anthraniloyl-L-alpha,beta-diaminopropionic acid (atnDap).
authors.affiliation: Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, 3-1-1 Tsushimanaka, Okayama 700-8530, Japan.
authors.forename: Masumi
authors.initials: M
authors.lastname: Taki
authors.forename: Takahiro
authors.initials: T
authors.lastname: Hohsaka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Murakami
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kuno
authors.forename: Tsunemi
authors.initials: T
authors.lastname: Hasegawa
authors.forename: Kazunari
authors.initials: K
authors.lastname: Taira
authors.forename: Masahiko
authors.initials: M
authors.lastname: Sisido
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D015153:Blotting, Western; D005456:Fluorescent Dyes / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D005456:Fluorescent Dyes; D019809:Streptavidin