Cellular responses to increasing Cd concentrations in the freshwater crab, Potamonautes warreni, harbouring microbial gill infestations.
We investigated the uptake, transport, storage and defence mechanisms in the freshwater crab, Potamonautes warreni, harbouring microbial gill infestations and exposed to increasing chronic (0.2, 0.5, 1.0 mg l(-1)) and acute (2.0 mg l(-1)) cadmium (Cd) concentrations under controlled laboratory conditions over a period of 21 days. Transmission electron microscopy and X-ray microanalysis revealed that the microbial gill fauna was eliminated on exposure to 0.2 mg Cd(2+) l(-1) and that Cd became increasingly adsorbed and incorporated into lamellar crystal deposits and permeated the cuticle of the gills of P. warreni. Degeneration of the apical membrane infoldings and vacuolation of epithelial cells occurred concurrently with pinocytosis, endocytosis and pronounced phagocytotic activity in the epithelia and haemal canal of the gills. Elevated Cd exposures (0.5 or 1.0 mg l(-1)) resulted in the swelling and dissociation of mitochondrial outer membranes together with an increase in transport of Cu, Cl and S by haemocytes in the haemal canal to epithelial tissues depleted in these elements. Cd also accumulated in tightly coiled concentric membrane whorls in the haemal canal, whereas the highest concentrations of Cd were found within aggregates of lysosome-like bodies in cuticulin-secreting cells of the gill stem. Chronic exposure to Cd induced increased fatigue and mild uncoordinated motor activity. In contrast, at an acute exposure of 2.0 mg l(-1) over 48 h, P. warreni showed a time-specific rapid loss of motor function, although only mild cellular lesions occurred in the gill tissues. The significance of cellular changes in the gill epithelia and altered motor activity of P. warreni with increased waterborne Cd are discussed as potential biomarker responses in monitoring aquatic pollution.
Schuwerack
P-Manuela M
PM
Department of Marine Science, Britannia Royal Naval College, Dartmouth, Devon, TQ6 0HJ, UK. ms@brnc.ac.uk
Lewis
John W
JW
D000818:Animals; D001419:Bacteria / Q000472:pathogenicity*; D001522:Behavior, Animal / Q000187:drug effects; D003386:Brachyura / Q000382:microbiology / Q000502:physiology*; D002104:Cadmium / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005618:Fresh Water; D005880:Gills / Q000382:microbiology* / Q000473:pathology / Q000648:ultrastructure; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D014874:Water Pollutants, Chemical; D002104:Cadmium