[A study on cellular matrix via cell extraction from bovine pericardium].
OBJECTIVE
To obtain an acellular matrix from bovine pericardial tissue, as a scaffold for tissue engineering of heart valves or cardiovascular patching applications.


METHODS
A four step detergent and enzyme link extraction procedure was practiced in this study. The mechanic properties of the acellular matrix were evaluated, and the components were analyzed biochemically.


RESULTS
HE staining confirmed the removal of cells and Von Gieson staining showed the integrality of collagen and elastin. Biochemical analysis demonstrated the retention of collagen and some glycosaminoglycans while the percentage of the soluble proteins reduced slightly. The tissue fracture strength and shrinkage temperature made no significant difference after extraction.


CONCLUSIONS
The extraction process is effective to remove cells from bovine pericardial tissue while maintains its mechanical strength. This approach may eventually lead to a scaffold for heart valves and cardiovascular patching applications in tissue engineering.
Zhou
J Y
JY
Division of Cardiac Valve, Fuwai Cardiovascular Institute, CAMS, PUMC, Beijing 100037, China. zhoujianye@china.com
Hu
S S
SS
Ren
B
B
Qi
G Q
GQ
D000818:Animals; D001705:Bioprosthesis; D002417:Cattle; D016238:Cytapheresis / Q000379:methods; D005109:Extracellular Matrix / Q000737:chemistry* / Q000648:ultrastructure; D006350:Heart Valve Prosthesis; D010496:Pericardium / Q000737:chemistry* / Q000648:ultrastructure; D023822:Tissue Engineering