Characterization of a white bass (Morone chrysops) embryonic cell line with epithelial features.
The genus Morone is an important one for U.S. aquaculture, but there has been no available cell line from this genus. We report here a cell line (the WBE line) derived from white bass embryos that has been grown for more than 80 passages over 21 mo in Dulbecco modified Eagle medium supplemented with fetal bovine serum. The WBE line showed epithelial features with positive immunohistochemical staining for cytokeratin and intercellular junctions when observed by electron microscopy. The doubling time, transformation characteristics, response to cryopreservation, and karyotype were determined. The WBE line was also tested by polymerase chain reaction to verify the species of origin and to screen for mycoplasma infection. This epithelial cell line may prove useful for the study of host-pathogen interactions that occur at the epithelial surface in this commercially important fish species.
Shimizu
Chisato
C
Department of Pediatrics, UCSD School of Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093-0830, USA.
Shike
Hiroko
H
Malicki
Denise M
DM
Breisch
Eric
E
Westerman
Mark
M
Buchanan
John
J
Ligman
Heather R
HR
Phillips
Ruth B
RB
Carlberg
James M
JM
Van Olst
Jack
J
Burns
Jane C
JC
D000818:Animals; D001492:Bass / Q000196:embryology*; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division; D002460:Cell Line; D048429:Cell Size; D002875:Chromosomes; D004625:Embryo, Nonmammalian / Q000166:cytology*; D004847:Epithelial Cells / Q000378:metabolism / Q000648:ultrastructure