Influence of skeletal muscle satellite cells implanted into infarcted myocardium on remnant myocyte volumes.
OBJECTIVE
To study the effects of skeletal muscle satellite cells implanted into infarcted myocardium on the volume of remnant myocytes.


METHODS
Thirty-six adult mongrel canines were divided randomly into implantation group and control group. In the implantation group, skeletal muscle satellite cells taken from the gluteus maximus muscles of the dogs were cultured, proliferated and labeled with 4',6-diamidino-2-phenylindone (DAPI) in vitro. In both groups, a model of acute myocardial infarction was established in every dog. In the implantation group, each dog was injected with M199 solution containing autologous skeletal muscle satellite cells. The dogs in the control group received M199 solution without skeletal muscle satellite cells. The dogs of both groups were killed 2, 4 and 8 weeks after implantation (six dogs in a separate group each time). Both infarcted myocardium and normal myocytes distal from the infracted regions isolated were observed under optical and fluorescent microscope. Their volumes were determined using a confocal microscopy image analysis system and analyzed using SAS. A P < 0.05 was considered significant.


RESULTS
A portion of the implanted cells differentiated into muscle fiber with striations and were connected with intercalated discs. Cross-sectional area and cell volume were increased in normal myocardium. Hypertrophy of remnant myocytes in the infarcted site after skeletal muscle cell implantation was much more evident than in the control group. Cross-sectional area, cell area and cell volume differed significantly from those of the control group (P < 0.05). Hypertrophy of the cells occurred predominantly in terms of width and thickness, whereas cell length remained unchanged.


CONCLUSIONS
Skeletal muscle satellite cells implanted into infarct myocardium, could induce the hypertrophy of remnant myocyte cells in the infarcted site and could also aid in the recovery of the contractile force of the infarcted myocardium.
Zhong
Hong
H
Department of Cardiothoracic Surgery, Xinhua Hospital, Shanghai Second Medical University, Shanghai 200092, China. zhonghongyy@21cn.com
Zhu
Hongsheng
H
Wei
Hongchao
H
Zhang
Zhen
Z
D000818:Animals; D048429:Cell Size; D004285:Dogs; D009203:Myocardial Infarction / Q000473:pathology*; D009206:Myocardium / Q000473:pathology*; D032383:Myocytes, Cardiac / Q000166:cytology; D011897:Random Allocation; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology / Q000502:physiology*