Mutagenicity of 5-formyluracil in mammalian cells.
5-Formyluracil, a major oxidized form of thymine, was incorporated into a predetermined site of one of the leading and lagging template strands of a double-stranded vector, and the DNA replication efficiency and the mutation frequency of 5-formyluracil in simian COS-7 cells were investigated. 5-Formyluracil did not block DNA replication and was weakly mutagenic in simian cells. 5-Formyluracil primarily elicited base substitutions at the modified positions.
Kamiya
H
H
Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan.
Murata-Kamiya
N
N
Karino
N
N
Ueno
Y
Y
Matsuda
A
A
Kasai
H
H
D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002522:Chlorocebus aethiops; D004247:DNA / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D004249:DNA Damage; D009153:Mutagens / Q000378:metabolism* / Q000633:toxicity*; D009838:Oligodeoxyribonucleotides / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism / Q000633:toxicity; D014498:Uracil / Q000031:analogs & derivatives* / Q000378:metabolism* / Q000633:toxicity*
D009153:Mutagens; D009838:Oligodeoxyribonucleotides; D017382:Reactive Oxygen Species; C007777:5-formyluracil; D014498:Uracil; D004247:DNA