title: [Linkage disequilibrium analysis of the single nucleotide polymorphisms in the PRKCZ gene].
abstract: OBJECTIVE
  To search for the disease-associated haplotype in the PRKCZ gene, a susceptibility gene for type 2 diabetes in Han population of North China, by case-control study and linkage disequilibrium (LD) analysis using single nucleotide polymorphisms (SNPs).
  
  
  METHODS
  SNPs located in the PRKCZ gene were chosen from public SNP domain by bioinformatic methods and single base extension (SBE) method was used to genotype the loci in 173 sporadic type 2 diabetes patients and 152 normal individuals to perform case-control study and LD analysis. Haplotype block were constructed in these populations.
  
  
  RESULTS
  Several SNPs in the PRKCZ gene were found to be associated with the disease. The SNPs formed different haplotype block pattern in case and control groups. The frequencies of the haplotypes formed by 5 SNPs were statistically different between the two groups.
  
  
  CONCLUSIONS
  The haplotype formed by 5 SNPs in the PRKCZ gene may be associated with type 2 diabetes in Han population of China, which is confirmed from statistics to be a susceptibility gene for the disease.
authors.affiliation: National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China.
authors.forename: Hong-xia
authors.initials: HX
authors.lastname: Sun
authors.forename: Wei-nan
authors.initials: WN
authors.lastname: Du
authors.forename: Yun-feng
authors.initials: YF
authors.lastname: Li
authors.forename: Jin
authors.initials: J
authors.lastname: Zuo
authors.forename: Guo-dong
authors.initials: GD
authors.lastname: Wu
authors.forename: Yan
authors.initials: Y
authors.lastname: Shen
authors.forename: Bo-qin
authors.initials: BQ
authors.lastname: Qiang
authors.forename: Zhi-jian
authors.initials: ZJ
authors.lastname: Yao
authors.forename: Jian-mei
authors.initials: JM
authors.lastname: Hang
authors.forename: Hui-yuan
authors.initials: HY
authors.lastname: Luo
authors.forename: Heng
authors.initials: H
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Zhu
authors.initials: Z
authors.lastname: Chen
authors.forename: Fu-de
authors.initials: FD
authors.lastname: Fang
mesh_terms: D000483:Alleles; D044466:Asian People; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005006:Ethnicity; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D015810:Linkage Disequilibrium; D020641:Polymorphism, Single Nucleotide; D011493:Protein Kinase C / Q000235:genetics*; D051745:Protein Kinase C-delta
chemical_list: C497258:PRKCD protein, human; D011493:Protein Kinase C; D051745:Protein Kinase C-delta