Development of electrochemically gene-analyzing method using DNA-modified electrodes.
For electrochemical DNA sequencing, several redox-responsive uridines modified at C5 were synthesized and site-specifically incorporated into DNA. Self-assembled DNA monolayers were prepared on a gold electrode using the dsDNA containing modified uridines. By measuring differential pulse voltammetry (DPV) using DNA-modified electrodes, the electrochemical response was strongly affected by the site of the redox-responsive uridine in dsDNA.
Okamoto
Akimitsu
A
Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, SORST, Japan Science and Technology Corporation, Kyoto 606-8501, Japan.
Kanatani
Keiichiro
K
Saito
Isao
I
D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D006046:Gold; D010084:Oxidation-Reduction
D006046:Gold; D004247:DNA