[Screening of hypercoagulability-related genes in aorta of hypertriglyceridemic rats].
OBJECTIVE
The aim of this study was to screen hypercoagulability-related genes in aorta of hypertriglyceridemic rats.


METHODS
Suppression subtractive hybridization (SSH) was used to construct hypercoagulable rat cDNA subtractive library. Semi-quantitative RT-PCR was applied to identify differentially expressed genes.


RESULTS
Hypercoagulable rat cDNA subtractive library had been successfully constructed. Sequence analysis and homology searching of 20 clones showed that these clones represent 19 genes: 8 are known genes newly found to have a relationship to hypercoagulability and 11 are novel genes (ESTs) that had been deposited to GenBank. Three ESTs (HC002, HC057 and HC067) were confirmed to be over-expressed genes by semi-quantitative RT-PCR.


CONCLUSIONS
The constructed subtractive library is highly efficient and has laid a foundation for screening and cloning hypercoagulability-related novel genes of rat, HC002, HC057 and HC067 are probably related to hypercoagulability induced by hypertriglyceridemia.
Zhao
Yuhua
Y
Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Liu
Bingwen
B
Bai
Huai
H
D000818:Animals; D001011:Aorta / Q000378:metabolism*; D005796:Genes; D005820:Genetic Testing; D006824:Hybridization, Genetic; D015228:Hypertriglyceridemia / Q000235:genetics*; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019851:Thrombophilia / Q000235:genetics*