A validated 1H NMR method for the determination of the degree of deacetylation of chitosan.
A method for the determination of the degree of deacetylation (DDA) of chitosan by 1H NMR spectroscopy has been formally validated. Chitosans with DDA ranging from 48 to 100% have been used for the validation. The method is found to be simple, rapid and more precise than other known techniques like IR or titration for %DDA measurements. The precision, ruggedness, robustness, specificity, stability and accuracy of the technique are discussed in this paper.
Lavertu
M
M
Department of Chemical Engineering, Institute of Biomedical and Chemical Engineering, Ecole Polytechnique of Montréal, PO Box 6079, Station Center-Ville, Québec, Montréal, Canada.
Xia
Z
Z
Serreqi
A N
AN
Berrada
M
M
Rodrigues
A
A
Wang
D
D
Buschmann
M D
MD
Gupta
Ajay
A
D000107:Acetylation; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry*; D048271:Chitosan; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D011522:Protons; D015203:Reproducibility of Results
D011522:Protons; D002686:Chitin; D048271:Chitosan