title: Two-terpyridine.Cu(II) complexes-containing antisense systems for rapid and highly site-specific RNA cleavage.
abstract: In an approach toward artificial ribonucleases, novel RNA cleaving systems were constructed that contained two terpyridine.Cu(II) residues. The first antisense system used tandem Cu(II) complex--2'-O-methyloligonucleotide 5'- and 3'-conjugates to cleave an RNA substrate. The second system, which will be described in a future paper, contained two contiguous Cu(II) complex residues at an internal site of a 2'-O-methyloligonucleotide. We found that the first system rapidly cleaved RNA with high site-specificity. Based on these results, we expect the second system to also show efficient RNA cleavage.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Inoue
authors.forename: T
authors.initials: T
authors.lastname: Furukawa
authors.forename: T
authors.initials: T
authors.lastname: Tamura
authors.forename: Y
authors.initials: Y
authors.lastname: Komatsu
authors.forename: E
authors.initials: E
authors.lastname: Ohtsuka
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D003300:Copper / Q000737:chemistry; D015195:Drug Design; D066298:In Vitro Techniques; D015394:Molecular Structure; D020320:Oligoribonucleotides, Antisense / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011725:Pyridines / Q000737:chemistry*; D012313:RNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D012260:Ribonucleases / Q000737:chemistry / Q000378:metabolism
chemical_list: D020320:Oligoribonucleotides, Antisense; D011725:Pyridines; D012313:RNA; D003300:Copper; D012260:Ribonucleases