title: Quality control of translation through the kinetic discrimination of tRNAs in the network of aminoacyl-tRNA synthetases.
abstract: It is known that each aminoacyl-tRNA synthetase (aaRS) specifically recognizes its cognate tRNAs to ensure the correct translation of the genetic information. However, we had previously demonstrated that mammalian mitochondrial seryl-tRNA synthetase (mt SerRS) can markedly misacylate mitochondrial (mt) tRNA(Gln). To investigate extensively misacylation reactions in mammalian mitochondrion, we purified overall twenty-two mt tRNAs from bovine liver, and determined their misacylation activities using mt SerRS. In addition to tRNA(Gln), tRNA(Ala) and tRNA(Asn) showed weak but significant serylation activities, which raises the possibility that each mammalian mt aaRS can misacylate several non-cognate mt tRNAs in varying degrees, but translational fidelity might be maintained by kinetic discrimination of tRNAs in the network of aaRSs.
authors.affiliation: Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
authors.forename: Nobukazu
authors.initials: N
authors.lastname: Shimada
authors.forename: Keiko
authors.initials: K
authors.lastname: Matsuzaki
authors.forename: Takeo
authors.initials: T
authors.lastname: Suzuki
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Suzuki
authors.forename: Kimitsuna
authors.initials: K
authors.lastname: Watanabe
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D007700:Kinetics; D014176:Protein Biosynthesis; D011786:Quality Control; D012343:RNA, Transfer / Q000378:metabolism*
chemical_list: D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases