title: Mutation analysis of CTNNB1 (beta-catenin) and AXIN1, the components of Wnt pathway, in cervical carcinomas.
abstract: The components of the Wnt-signaling pathway are mutated in tumors, but the relationship between these components and cervical cancer has not been elucidated. In this study, we used immunohistochemistry, single strand confirmation polymorphism (SSCP) and direct sequencing methods to analyze the mutation and protein expressions of both CTNNB1 and AXIN1 in cervical cancer. Among the 30 tested cervical cancers, no mutation of CTNNB1 but 3 polymorphisms were found. Mutation analysis of AXIN1 revealed that one specimen had a heterozygous mutation at codon 740 (GCC right curved arrow ACC) and six polymorphisms were also found. Immunohistochemistry showed no relationship between the protein expression patterns and mutation of AXIN1 and CTNNB1. Mutations of CTNNB1 may not be a factor, whereas mutations of AXIN1 may play a limited role in tumorigenesis of cervical cancer. In addition, aberrant expression patterns are not mutation related, so that other factors may be responsible for these changes.
authors.affiliation: Department of Molecular Medicine, China Medical College Hospital, Taichung, Taiwan.
authors.forename: Tsung-Hsien
authors.initials: TH
authors.lastname: Su
authors.forename: Jan-Gowth
authors.initials: JG
authors.lastname: Chang
authors.forename: Kun-Tu
authors.initials: KT
authors.lastname: Yeh
authors.forename: Ting-Hsu
authors.initials: TH
authors.lastname: Lin
authors.forename: Tai-Ping
authors.initials: TP
authors.lastname: Lee
authors.forename: Jui-Chang
authors.initials: JC
authors.lastname: Chen
authors.forename: Cheng-Chieh
authors.initials: CC
authors.lastname: Lin
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D060466:Axin Protein; D001483:Base Sequence; D003062:Codon; D004247:DNA / Q000737:chemistry; D004252:DNA Mutational Analysis; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D008969:Molecular Sequence Data; D009154:Mutation; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000378:metabolism*; D051153:Wnt Proteins; D029961:Zebrafish Proteins
chemical_list: C555451:AXIN1 protein, human; D060466:Axin Protein; D003062:Codon; D017931:DNA Primers; D011518:Proto-Oncogene Proteins; D012097:Repressor Proteins; D051153:Wnt Proteins; D029961:Zebrafish Proteins; D004247:DNA