[Establishment and preliminary application of screening methods for Y chromosome microdeletions in male infertility patients].
OBJECTIVE
To develop a multiplex PCR protocol, which could be suitable for routine screening of microdeletions on the Y chromosome in azoospermic and oligozoospermic male infertility patients.


METHODS
Five multiplex sets were established. Eighty-seven azoospermic and oligozoospermic patients undergoing intracytoplasmic sperm injection (ICSI) in the in vitro fertilization (IVF) center and 30 azoospermic men undergoing testicular biopsy in the clinic of Urology Surgery were screened for microdeletions of Y chromosome.


RESULTS
A total of 19 (16.2%) cases of microdeletions were found in 117 azoospermic and oligozoospermic patients by screening of Y chromosome microdeletions. Of these, 11 cases (18.0%) were found in 61 oligozoospermic patients, and 8 cases (14.3%) were found in 56 azoospermic patients.


CONCLUSIONS
The multiplex PCR protocol presented in this study is an easy-to-do and reliable method for detecting microdeletions on the Y chromosome. Routine screening of microdeletions on the Y chromosome for azoospermic and oligozoospermic patients is essential.
Yu
Cong-yi
CY
Department of Obstetrics and Gynecology, Reproductive Medical Center, the Second Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, 510120 PR China. yucongyi01@sohu.com
Zhuang
Guang-lun
GL
Zhou
Can-quan
CQ
Yan
Zong-he
ZH
Li
Wei
W
Gao
Hua
H
Rui
De-rong
DR
D002872:Chromosome Deletion; D041322:Chromosomes, Human, Y / Q000235:genetics*; D005260:Female; D005820:Genetic Testing / Q000379:methods; D006801:Humans; D007248:Infertility, Male / Q000175:diagnosis / Q000235:genetics*; D008297:Male; D016133:Polymerase Chain Reaction