Extension of biochemical functions by the introduction of nonnatural amino acids and artificial nucleic acid analogs.
Extension of biochemical functions has been attempted by introducing nonatural amino acids and artificial nucleic acid analogs. Nonnatural amino acids have been linked to tRNAs and the amino-acylated tRNAs were added to E. coli in vitro protein synthesizing system to produce nonnatural mutant proteins. The positions of the nonnatural amino acids have been assigned by the 4-base codons, like CGGG and AGGU. The extended codons have been introduced at a specific position or at random positions on a DNA. In the latter case, a DNA library that contains a single 4-base codon at random positions can be obtained. The combination of these new techniques opens a way to the introduction of artificial functions to biochemical systems.
Sisido
M
M
Department of Bioscience and Biotechnology, Okayama University, 3-1-1 Tsushimanaka, Okayama 700-8530, Japan.
D000596:Amino Acids / Q000737:chemistry* / Q000378:metabolism*; D001483:Base Sequence; D003062:Codon / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005456:Fluorescent Dyes; D016296:Mutagenesis; D016254:Mutagenesis, Insertional; D009696:Nucleic Acids / Q000737:chemistry* / Q000378:metabolism*; D020135:Peptide Nucleic Acids / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
D000596:Amino Acids; D003062:Codon; D005456:Fluorescent Dyes; D009696:Nucleic Acids; D020135:Peptide Nucleic Acids; D011994:Recombinant Proteins