Systematic characterization of Escherichia coli genes/ORFs affecting biofilm formation.
To understand the nature and function of bacterial biofilm and the process of its formation, we have performed systematic screening of a complete set of Escherichia coli genes/open reading frames (ORFs) to identify those that affect biofilm development upon over-expression. In contrast to the biofilm of strain AG1 used as a control, some of the genes/ORFs when over-expressed led to the formation of an abnormal biofilm such as thin, mat-like, filamentous or one easily detaching from various surfaces. Disruptants of selected genes were constructed in order to clarify their roles in the different stages of biofilm formation. Our results suggest that diverse metabolic pathways contribute to the development of biofilm.
Tenorio
Elizabeth
E
Graduate School of Science and Technology, Kobe University, Kobe, Japan.
Saeki
Takashi
T
Fujita
Katsutoshi
K
Kitakawa
Madoka
M
Baba
Tomoya
T
Mori
Hirotada
H
Isono
Katsumi
K
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D018441:Biofilms / Q000254:growth & development*; D004926:Escherichia coli / Q000235:genetics* / Q000502:physiology*; D010861:Fimbriae, Bacterial / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D016296:Mutagenesis; D016366:Open Reading Frames; D010641:Phenotype
D001425:Bacterial Outer Membrane Proteins