Trans-kingdom conjugation between bacterial chromosome-less mini-cells and yeasts.
We introduced a novel convenient method to prepare chromosome-less mini-cells from cell mixture of Escherichia coli P678-54 by using penicillin. The mini-cells prepared by this method could successfully transferred their harboring conjugative plasmids into eukaryotic yeast cells by trans-kingdom conjugation.
Fukumaru
K
K
Department of Biological Science, Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8526, Japan.
Yoshida
K
K
D002876:Chromosomes, Bacterial / Q000235:genetics; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000235:genetics*; D005821:Genetic Techniques; D008856:Microscopy, Fluorescence; D010406:Penicillins; D010957:Plasmids / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D010406:Penicillins