Dexamethasone induced preadipocyte recruitment and expression of CCAAT/enhancing binding protein alpha and peroxisome proliferator activated receptor-gamma proteins in porcine stromal-vascular (S-V) cell cultures obtained before and after the onset of fetal adipogenesis.
The present study examined the influence of dexamethasone (DEX) treatment on preadipocyte recruitment and expression of transcription factor proteins in adipose tissue stromal-vascular (S-V) cell cultures from 50 and 75 day old pig fetuses and young pigs. C/EBPalpha, C/EBPdelta, and PPARgamma immunoreactive cells had evenly reactive nuclei and unreactive nucleoli. DEX recruited many more preadipocytes in 75 day than in 50 day fetal S-V cultures. However, DEX did not increase the number of differentiated preadipocytes (lipid+, C/EBPalpha+) in 50 day S-V cultures and only slightly increased this number in 75 day fetal S-V cultures. In fetal cultures, extensive, precocious increases in C/EBPalpha expression (number of reactive cells) by day three were followed by extensive decreases in expression. However, PPARgamma expression was not expressed precociously since preadipocyte lipid accretion and PPARgamma immunoreactivity were strongly linked in fetal and pig S-V cultures. Nevertheless, all cells with lipid in fetal S-V cultures were C/EBPalpha and PPARgamma reactive. DEX increases preadipocyte differentiation in pig S-V cultures and in this study DEX increased PPARgamma expression to a much greater degree in pig than in fetal S-V cultures. These studies suggest that restricted adipogenesis in the pig fetus is attributable to limited DEX induced PPARgamma expression.
Hausman
G J
GJ
USDA-ARS, Richard B Russell Agricultural Research Center, Animal Physiology Research Unit, 950 College Station Road, Athens, GA 30605-2720, USA. ghausman@saa.as.usda.gov
D017667:Adipocytes / Q000502:physiology*; D000273:Adipose Tissue / Q000196:embryology*; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D001808:Blood Vessels / Q000166:cytology / Q000378:metabolism; D015153:Blotting, Western; D022762:CCAAT-Enhancer-Binding Proteins / Q000378:metabolism*; D002452:Cell Count; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology*; D005314:Embryonic and Fetal Development; D005938:Glucocorticoids / Q000494:pharmacology*; D007150:Immunohistochemistry; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D013234:Stem Cells / Q000502:physiology*; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism; D013552:Swine; D014157:Transcription Factors / Q000378:metabolism*
D022762:CCAAT-Enhancer-Binding Proteins; D005938:Glucocorticoids; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors; D003907:Dexamethasone