NMR study of a novel RNA quadruplex structure.
The structure of an RNA oligomer, r (GGAGGUUUUGGAGG) (R14-2) whose G-G steps are separated by adenine and uracil residues has been investigated by NMR. In the presence of 20 mM K+, a novel dimeric multiplex architecture is adopted by two strands of R14-2. In each strand a UUUU loop and two A residues connect four parallel G-G steps that pair-align into two tetrads. One of the tetrads is further pair-aligned by two A residues through the sheared mismatch and a novel hexad is subsequently formed. Two hexads coming from two different strands stack to make a dimeric multiplex. All of the guanosine and adenosine residues take an anti conformation.
Liu
H
H
Department of Chemistry and Biotechnology, Faculty of Engineering, Yokohama National University.
Kanagawa
M
M
Matsugami
A
A
Tanaka
Y
Y
Katahira
M
M
Uesugi
S
S
D001483:Base Sequence; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000737:chemistry; D012313:RNA / Q000737:chemistry*
D009843:Oligoribonucleotides; D012313:RNA