Novel approach for SNP genotyping based on site-selective RNA scission.
Novel genotyping method for single nucleotide polymorphisms (SNPs), based on site-selective RNA scission, has been developed. A substrate RNA is activated at two sites by complementary acridine-modified DNA having two acridine residues, and is site-selectively cleaved by metal ion catalyst to produce short RNA fragment containing the SNP site. Genotype of the substrate is accurately and easily determined by mass analysis of the fragment.
Kuzuya
Akinori
A
Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
Mizoguchi
Ryo
R
Morisawa
Fumi
F
Komiyama
Makoto
M
D001483:Base Sequence; D005838:Genotype; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D012313:RNA / Q000235:genetics*
D012313:RNA