title: Loss of intracellular dystrophin: a potential mechanism for myocardial reperfusion injury.
abstract: Because the absence of sarcolemmal dystrophin renders cardiomyocytes vulnerable to mechanical force, the present study investigated whether sarcolemmal membrane fragility upon reperfusion is associated with the loss of membrane dystrophin. Dystrophin was distributed exclusively in the sarcolemmal membrane of buffer-perfused rat cardiomyocytes, but was translocated to the myofibrils during 30 min of ischemia and then lost during reperfusion. Upon reperfusion, the membrane impermeable dye, Evans blue (EB), accumulated in cardiomyocytes depleted of dystrophin. Reperfusion with the contractile blocker 2,3-butanedione monoxime (BDM) resulted in no accumulation of EB in cardiomyocytes despite the loss of dystrophin. Upon withdrawal of BDM, however, EB accumulated in dystrophin-depleted cardiomyocytes. Loss of sarcolemmal dystrophin may be involved in the mechanism of contractile force-induced reperfusion injury.
authors.affiliation: The Cardiovascular Center, Kansai Medical University, Moriguchi, Japan.
authors.forename: Shiori
authors.initials: S
authors.lastname: Kyoi
authors.forename: Hajime
authors.initials: H
authors.lastname: Otani
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Sumida
authors.forename: Takayuki
authors.initials: T
authors.lastname: Okada
authors.forename: Motohiko
authors.initials: M
authors.lastname: Osako
authors.forename: Hiroji
authors.initials: H
authors.lastname: Imamura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kamihata
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Matsubara
authors.forename: Toshiji
authors.initials: T
authors.lastname: Iwasaka
mesh_terms: D000818:Animals; D001692:Biological Transport; D004396:Coloring Agents / Q000493:pharmacokinetics; D003931:Diacetyl / Q000031:analogs & derivatives* / Q000494:pharmacology; D016189:Dystrophin / Q000172:deficiency*; D005070:Evans Blue / Q000493:pharmacokinetics; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D008297:Male; D009200:Myocardial Contraction / Q000187:drug effects; D017202:Myocardial Ischemia / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000209:etiology*; D009206:Myocardium / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000378:metabolism; D009210:Myofibrils / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012508:Sarcolemma / Q000378:metabolism*
chemical_list: D004396:Coloring Agents; D016189:Dystrophin; C004717:diacetylmonoxime; D005070:Evans Blue; D003931:Diacetyl