title: The influence of omega-3 polyunsaturated fatty acids (omega-3 pufa) on lactobacilli adhesion to the intestinal mucosa and on immunity in gnotobiotic piglets.
abstract: The effect of application of omega-3 polyunsaturated fatty acids (omega-3 PUFA) on intestinal colonization by Lactobacillus paracasei and on cellular immunity has been investigated in gnotobiotic pigs. The administration of polyunsaturated fatty acids positively affected the adhesion of Lactobacillus paracasei to the jejunal mucosa of gnotobiotic piglets. When compared to the control group, the number of Lactobacillus paracasei adhering to the jejunal mucosa was by 12% higher in piglets of the experimental group (5.10 log 10/cm2 vs. 4.55 log 10/cm2). The respective counts of Lactobacillus paracasei adhering to the ileal and colonic mucosa of 28 day old gnotobiotic piglets reached 4.45 and 5.05 log 10/cm2 in group C and 4.44 and 4.95 log 10/cm2 in group E. Omega-3 PUFA supplementation increased the phagocytic activity of neutrophils by almost 100% on day 28 of life as well as the subpopulations of lymphocytes (CD8) in the peripheral blood of germ-free piglets on day 21 of life. Our results indicate that the action of probiotics in the gut may be modulated by dietary PUFA. The stimulatory effect of PUFA upon adhesion of lactobacilli could be used for enhancing the effectiveness of probiotics in inhibiting digestive tract pathogens.
authors.affiliation: University of Veterinary Medicine, Kosice, Slovak Republic.
authors.forename: Alojz
authors.initials: A
authors.lastname: Bomba
authors.forename: Radomíra
authors.initials: R
authors.lastname: Nemcová
authors.forename: Sona
authors.initials: S
authors.lastname: Gancarcíková
authors.forename: Robert
authors.initials: R
authors.lastname: Herich
authors.forename: Juraj
authors.initials: J
authors.lastname: Pistl
authors.forename: Viera
authors.initials: V
authors.lastname: Révajová
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Jonecová
authors.forename: Andrej
authors.initials: A
authors.lastname: Bugarský
authors.forename: Mikulás
authors.initials: M
authors.lastname: Levkut
authors.forename: Rudolf
authors.initials: R
authors.lastname: Kasteĺ
authors.forename: Miroslav
authors.initials: M
authors.lastname: Baran
authors.forename: Gabriel
authors.initials: G
authors.lastname: Lazar
authors.forename: Marián
authors.initials: M
authors.lastname: Hluchý
authors.forename: Slávka
authors.initials: S
authors.lastname: Marsálková
authors.forename: Ján
authors.initials: J
authors.lastname: Posivák
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects*; D015169:Colony Count, Microbial; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D005260:Female; D005856:Germ-Free Life; D007413:Intestinal Mucosa / Q000382:microbiology*; D007778:Lactobacillus / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D019936:Probiotics; D011897:Random Allocation; D013552:Swine / Q000276:immunology / Q000382:microbiology*
chemical_list: D015525:Fatty Acids, Omega-3