Altered inhibitory input to Purkinje cells of dystrophin-deficient mice.
We investigated evoked EPSPs and spontaneous IPSPs in cerebellar slices from dystrophin-deficient mdx mice. In the presence of the GABA(A) antagonist bicuculline the increase in EPSP amplitude was less in mdx Purkinje cells compared to control, and the amplitude of miniature IPSCs in mdx cells was also significantly less than in controls. This reduced inhibitory input is most likely due to the reported reduction in the size of GABA(A) channel clusters.
Anderson
Jennifer L
JL
School of Medical Sciences, University of New South Wales, Sydney 2052, Australia.
Head
Stewart I
SI
Morley
John W
JW
D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D002531:Cerebellum / Q000187:drug effects / Q000378:metabolism; D016189:Dystrophin / Q000172:deficiency* / Q000378:metabolism; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D009433:Neural Inhibition / Q000187:drug effects / Q000502:physiology*; D011689:Purkinje Cells / Q000187:drug effects / Q000378:metabolism*
D016189:Dystrophin; D001640:Bicuculline