title: Post-integration behavior of a Mos1 mariner gene vector in Aedes aegypti.
abstract: The post-integration behavior of insect gene vectors will determine the types of applications for which they can be used. Transposon mutagenesis, enhancer trapping, and the use of transposable elements as genetic drive systems in insects requires transposable elements with high rates of remobilization in the presence of transposase. We investigated the post-integration behavior of the Mos1 mariner element in transgenic Aedes aegypti by examining both germ-line and somatic transpositions of a non-autonomous element in the presence of Mos1 transposase. Somatic transpositions were occasionally detected while germ-line transposition was only rarely observed. Only a single germ-line transposition event was recovered after screening 14,000 progeny. The observed patterns of transposition suggest that Mos1 movement takes place between the S phase and anaphase. The data reported here indicate that Mos1 will be a useful vector in Ae. aegypti for applications requiring a very high degree of vector stability but will have limited use in the construction of genetic drive, enhancer trap, or transposon tagging systems in this species.
authors.affiliation: Center for Biosystems Research, University of Maryland Biotechnology Institute, College Park, MD 20742-4450, USA.
authors.forename: Raymond
authors.initials: R
authors.lastname: Wilson
authors.forename: Jamison
authors.initials: J
authors.lastname: Orsetti
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Klocko
authors.forename: Channa
authors.initials: C
authors.lastname: Aluvihare
authors.forename: Edward
authors.initials: E
authors.lastname: Peckham
authors.forename: Peter W
authors.initials: PW
authors.lastname: Atkinson
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Lehane
authors.forename: David A
authors.initials: DA
authors.lastname: O'Brochta
mesh_terms: D000330:Aedes / Q000201:enzymology / Q000235:genetics*; D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D015139:Blotting, Southern; D004251:DNA Transposable Elements / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D017344:Genes, Insect / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D005854:Germ Cells / Q000502:physiology; D008938:Mitosis / Q000235:genetics; D010641:Phenotype; D011995:Recombination, Genetic; D019895:Transposases / Q000378:metabolism
chemical_list: D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; C111652:mariner transposases; D019895:Transposases