title: [Dependence of the expression of the biological features of Vibrio cholerae on the conditions of their cultivation].
abstract: The biological activity of toxigenic and non-toxigenic V. cholerae supernatants was found to depend on the cultivation medium. The use of iron-free tryptone medium made it possible to obtain supernatants of toxigenic V. cholerae with haemolytic activity and destructive action on passaged cell cultures. In the experimental infection of suckling rabbits the influence of the cultivation conditions of V. cholerae on the character and expression of their pathogenic properties was determined. The dissemination of V. cholerae into the internal organs of rabbits after their infection with both toxigenic and non-toxigenic strains correlated neither with the cultivation conditions of these strains, nor with the character of changes in the intestine of the infected animals.
authors.affiliation: Research Institute for Plague Control, Rostov-on-Don, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Mironova
authors.forename: E A
authors.initials: EA
authors.lastname: Menshikova
authors.forename: A V
authors.initials: AV
authors.lastname: Sokolenko
mesh_terms: D000818:Animals; D002771:Cholera / Q000382:microbiology; D003470:Culture Media; D006462:Hemolytic Plaque Technique; D007501:Iron; D011817:Rabbits; D014734:Vibrio cholerae / Q000254:growth & development / Q000472:pathogenicity*; D014774:Virulence / Q000502:physiology; D037521:Virulence Factors / Q000096:biosynthesis
chemical_list: D003470:Culture Media; D037521:Virulence Factors; D007501:Iron