Solid-phase synthesis of deoxyribonucleotides having a cap structure analog.
In this study, the solid-phase synthesis of oligodeoxyribonucleotides having a guanosine pyrophosphate cap structure (Gpp-) was achieved by using a new guanosine monophosphate unit having the DMTr group capable of estimation of coupling efficiency of the pyrophosphate bond formation. Since 7-methylguanosine base was unstable under basic conditions, Gpp-capped DNA oligomers were synthesized by using a new linker having a silanediyl bond, which allowed to release the DNA chain from the solid support by treatment with fluoride anion under neutral conditions.
Ushioda
M
M
Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Midoriku, Yokohama 226, Japan.
Kadokura
M
M
Murami
Y
Y
Moriguti
T
T
Wada
T
T
Seio
K
K
Sekine
M
M
D008722:Methods; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D012314:RNA Cap Analogs / Q000138:chemical synthesis* / Q000737:chemistry*
D009838:Oligodeoxyribonucleotides; D012314:RNA Cap Analogs