title: Multicomponent submicron features of biomolecules created by voltage controlled deposition from a nanopipet.
abstract: We have used a nanopipet as a nanopen to locally and controllably deposit complex biomolecules, including antibodies and DNA, onto a surface to create multicomponent and functional submicron features. Key advantages of this method are that the biomolecules are always in solution and the applied voltage provides fine control of the delivery down to the single molecule level. Two consecutive cycles of deposition, to produce spatially varying features with different biological properties, were demonstrated with fluorescently labeled antibodies or biotin. This approach combines "top-down" fabrication, using the nanopen for local application, and "bottom-up" fabrication, using molecular recognition for self-assembly at defined positions, and opens up new possibilities in nanotechnology.
authors.affiliation: Department of Chemistry, University of Cambridge, United Kingdom.
authors.forename: Andreas
authors.initials: A
authors.lastname: Bruckbauer
authors.forename: Dejian
authors.initials: D
authors.lastname: Zhou
authors.forename: Liming
authors.initials: L
authors.lastname: Ying
authors.forename: Yuri E
authors.initials: YE
authors.lastname: Korchev
authors.forename: Chris
authors.initials: C
authors.lastname: Abell
authors.forename: David
authors.initials: D
authors.lastname: Klenerman
mesh_terms: D000818:Animals; D001710:Biotin / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D006041:Goats; D007074:Immunoglobulin G / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D011817:Rabbits
chemical_list: D005456:Fluorescent Dyes; D007074:Immunoglobulin G; D001710:Biotin; D004247:DNA