title: Synthesis and properties of phosphorylated 3'-O-beta-D-ribofuranosyl-2'-deoxythymidine.
abstract: A strategy was developed for the synthesis of 3'-O-beta-D-ribofuranosyl 2'-deoxythymidine derivatives using three different protecting groups, which allows the synthesis of a phosphoramidite building block for oligonucleotide synthesis. Likewise the 5'-O- and 5''-O-phosphorylated analogues were synthesized and their conformation was determined using NMR spectroscopy.
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Ekaterina V
authors.initials: EV
authors.lastname: Efimtseva
authors.forename: Alexandra A
authors.initials: AA
authors.lastname: Shelkunova
authors.forename: Sergey N
authors.initials: SN
authors.lastname: Mikhailov
authors.forename: Koen
authors.initials: K
authors.lastname: Nauwelaerts
authors.forename: Jef
authors.initials: J
authors.lastname: Rozenski
authors.forename: Eveline
authors.initials: E
authors.lastname: Lescrinier
authors.forename: Piet
authors.initials: P
authors.lastname: Herdewijn
mesh_terms: D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000737:chemistry; D010766:Phosphorylation; D013936:Thymidine / Q000138:chemical synthesis* / Q000737:chemistry*
chemical_list: D009841:Oligonucleotides; D009943:Organophosphorus Compounds; C434331:phosphoramidite; D013936:Thymidine