title: The cellular distribution and oxidation state of platinum(II) and platinum(IV) antitumour complexes in cancer cells.
abstract: The cellular distribution of platinum in A2780 ovarian cancer cells treated with cisplatin and platinum(IV) complexes with a range of reduction potentials has been examined using elemental analysis (synchrotron radiation-induced X-ray emission). The cellular distribution of platinum(IV) drugs after 24 h is similar to that of cisplatin, consistent with the majority of administered platinum(IV) drugs being reduced. Micro-X-ray absorption near-edge spectra of cells treated with cisplatin and platinum(IV) complexes confirmed the reduction of platinum(IV) to platinum(II). In cells treated, the most difficult to reduce complex, cis, trans, cis-[PtCl(2)(OH)(2)(NH(3))(2)], platinum(IV) was detected in the cells along with platinum(II). The observations are in accordance with the relative ease of reduction of the platinum(IV) complexes used and support the requirement of reduction for activation of platinum(IV) complexes.
authors.affiliation: Centre for Heavy Metals Research, School of Chemistry F11, The University of Sydney, 2006, N.S.W., Australia.
authors.forename: Matthew D
authors.initials: MD
authors.lastname: Hall
authors.forename: Carolyn T
authors.initials: CT
authors.lastname: Dillon
authors.forename: Mei
authors.initials: M
authors.lastname: Zhang
authors.forename: Philip
authors.initials: P
authors.lastname: Beale
authors.forename: Zhonghou
authors.initials: Z
authors.lastname: Cai
authors.forename: Barry
authors.initials: B
authors.lastname: Lai
authors.forename: Anton P J
authors.initials: AP
authors.lastname: Stampfl
authors.forename: Trevor W
authors.initials: TW
authors.lastname: Hambley
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000493:pharmacokinetics*; D001692:Biological Transport; D002451:Cell Compartmentation; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000737:chemistry / Q000493:pharmacokinetics*; D003952:Diagnostic Imaging; D005260:Female; D006801:Humans; D010051:Ovarian Neoplasms / Q000473:pathology*; D010084:Oxidation-Reduction; D017671:Platinum Compounds / Q000737:chemistry / Q000493:pharmacokinetics*; D013052:Spectrometry, X-Ray Emission; D013057:Spectrum Analysis; D014965:X-Rays
chemical_list: D000970:Antineoplastic Agents; D017671:Platinum Compounds; D002945:Cisplatin