title: CIITA-regulated plexin-A1 affects T-cell-dendritic cell interactions.
abstract: The major histocompatibility complex (MHC) class II transactivator (CIITA) is the 'master coactivator' of MHC class II genes. To identify new targets of CIITA, we analyzed cDNA microarrays of dendritic cells (DCs) from CIITA-deficient, MHC class II-deficient and control mice. We found the semaphorin receptor plexin-A1 was expressed in DCs, but not in other immune cells, and was strongly induced by CIITA. RNA interference by short hairpin RNA specific for plexin-A1, but not a single-nucleotide mutant, greatly reduced plexin-A1 expression and T cell stimulation by protein- or peptide-antigen-pulsed DCs.Plexin-A1 is not required for peptide binding to MHC. These data indicate involvement of plexin-A1 in T cell-DC interactions but not antigen processing or binding.
authors.affiliation: Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA.
authors.forename: Athena W
authors.initials: AW
authors.lastname: Wong
authors.forename: W June
authors.initials: WJ
authors.lastname: Brickey
authors.forename: Debra J
authors.initials: DJ
authors.lastname: Taxman
authors.forename: Hendrick W
authors.initials: HW
authors.lastname: van Deventer
authors.forename: William
authors.initials: W
authors.lastname: Reed
authors.forename: Jian Xin
authors.initials: JX
authors.lastname: Gao
authors.forename: Pan
authors.initials: P
authors.lastname: Zheng
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Janice S
authors.initials: JS
authors.lastname: Blum
authors.forename: Karen P
authors.initials: KP
authors.lastname: McKinnon
authors.forename: Jenny P-Y
authors.initials: JP
authors.lastname: Ting
mesh_terms: D000818:Animals; D001483:Base Sequence; D002450:Cell Communication / Q000276:immunology*; D003433:Crosses, Genetic; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D015536:Down-Regulation / Q000276:immunology; D005434:Flow Cytometry; D005802:Genes, MHC Class II; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000276:immunology*; D009687:Nuclear Proteins; D020411:Oligonucleotide Array Sequence Analysis; D011401:Promoter Regions, Genetic; D011956:Receptors, Cell Surface / Q000235:genetics / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D015534:Trans-Activators / Q000276:immunology*; D015533:Transcriptional Activation / Q000276:immunology
chemical_list: D018076:DNA, Complementary; C083728:MHC class II transactivator protein; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; C492114:Plxna1 protein, mouse; D011956:Receptors, Cell Surface; D015534:Trans-Activators