Possibility of using DNA chip technology for diagnosis of human papillomavirus.
To explore the application of DNA chip technology for the detection and typing of Human Papillomavirus (HPV), the HPV6, 11, 16 and 18 gene fragments were isolated and printed onto aminosilane-coated glass slides by a PixSys 5500 microarrayer as probes to prepare the HPV gene chips. HPV samples, after being labeled with fluorescent dye by restriction display PCR (RD-PCR) technology, were hybridized with the microarray, which was followed by scanning and analysis. The experimental condition for preparing the HPV gene chips was investigated, and the possibility of HPV genotyping using gene chips was discussed. The technique that was established in this study for preparing HPV gene chips is practical. The results of the present study demonstrated the versatility and inspiring prospect of using this technology to detect and genotype HPV.
Liu
Cui-hua
CH
Institute of Molecular Biology, First Uniform Medical University, Guangzhou 510515, China.
Ma
Wen-li
WL
Shi
Rong
R
Ou
Yang-qian
YQ
Zhang
Bao
B
Zheng
Wen-ling
WL
D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis; D005838:Genotype; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000175:diagnosis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D015342:DNA Probes; D004279:DNA, Viral