title: [Detection of ermB gene responsible for high level resistance to clindamycin (MLS type resistance) among Clostridium difficile strains isolated from antibiotic associated diarrhea (AAD)].
abstract: In 68 C. difficile strains isolated from feacal samples of patients with antibiotic associated diarrhoea (AAD) investigated presence of ermB gene transferable of high level resistance to clindamycin. The primers set 2980/2981 used for identification of ermB gene amplified a 688 bp segment. We used the Etest to assess all strains for susceptibility to clindamycin. This study demonstrates that 57% of strains isolated from faecal samples of patients with AAD were highly resistant to clindamycin (minimal inhibitory concentration (MIC) of clindamycin, 256 mg/L) and possessed the ermB gene.
authors.affiliation: Katedra i Zakład Mikrobiologii Lekarskiej AM w Warszawie.
authors.forename: Hanna
authors.initials: H
authors.lastname: Pituch
authors.forename: Piotr
authors.initials: P
authors.lastname: Obuch-Woszczatyński
authors.forename: Felicja
authors.initials: F
authors.lastname: Meisel-Mikołajczyk
authors.forename: Miroslaw
authors.initials: M
authors.lastname: Łuczak
mesh_terms: D002981:Clindamycin / Q000009:adverse effects / Q000494:pharmacology*; D016360:Clostridioides difficile / Q000187:drug effects* / Q000235:genetics*; D003967:Diarrhea / Q000139:chemically induced / Q000382:microbiology; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004761:Enterocolitis, Pseudomembranous / Q000139:chemically induced / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D006801:Humans; D008780:Methyltransferases / Q000302:isolation & purification*; D008826:Microbial Sensitivity Tests
chemical_list: D002981:Clindamycin; D008780:Methyltransferases; C035181:rRNA (adenosine-O-2'-)methyltransferase