title: Application of capillary electrophoresis with pH-mediated sample stacking to analysis of coumarin metabolites in microsomal incubations.
abstract: A sensitive method for the analysis of metabolites of coumarin by capillary electrophoresis (CE), incorporating pH-mediated sample stacking, was developed. The analytes were detected in phosphate buffer (pH 7.5; 25 mM), the matrix of the microsomal incubations. Detection was by direct UV absorbance. The three metabolites studied were 7-hydroxycoumarin (7-OHC), 4-hydroxycoumarin (4-OHC) and 2-hydroxyphenylacetic acid (HPAA), and the limits of detection of the analytes were 0.1, 0.5 and 0.3 microM, respectively. The developed method was then applied to microsomal incubations of coumarin. Male Cynomologus monkey microsomes were used in the study and 7-OHC was detected in the incubation mixture.
authors.affiliation: National Centre for Sensor Research, School of Chemical Sciences, Dublin City University, Dublin 9, Ireland.
authors.forename: Eimear M
authors.initials: EM
authors.lastname: Ward
authors.forename: Malcolm R
authors.initials: MR
authors.lastname: Smyth
authors.forename: Richard
authors.initials: R
authors.lastname: O'Kennedy
authors.forename: Craig E
authors.initials: CE
authors.lastname: Lunte
mesh_terms: D000818:Animals; D003374:Coumarins / Q000032:analysis* / Q000378:metabolism*; D019075:Electrophoresis, Capillary / Q000379:methods; D000882:Haplorhini; D006863:Hydrogen-Ion Concentration; D008297:Male; D008861:Microsomes / Q000737:chemistry* / Q000378:metabolism*; D013678:Technology, Pharmaceutical / Q000379:methods*
chemical_list: D003374:Coumarins; C030123:coumarin