title: [Arsenic trioxide induces human tumor cell apoptosis and G2 + M arrest whereas causes HPV16 DNA immortalized cervical epithelial cells G1 block].
abstract: OBJECTIVE
  To investigate whether As2O3 induces tumor cell apoptosis and cell cycle arrest.
  
  
  METHODS
  DNA agarose gel electrophoresis, flowcytometry analysis, RT-PCR, and Western blot.
  
  
  RESULTS
  As2O3 induced G2 + M arrest of tumor cells such as GLC-82, MGC-803, SGC-7901, Eca109 and HeLa cells before inducing apoptosis and decreased c-myc gene expression, whereas caused G1 block of HPV16 DNA immortalized cervical epithelial HCE16/3 cells and did not affect c-myc expression.
  
  
  CONCLUSIONS
  As2O3 caused apoptosis was related with cell cycle arrest. However, the phase of arrest of tumor cells was different from the pre-tumor cell HCE16/3 cells, which could be related to the change of c-myc gene expression.
authors.affiliation: National Laboratory of Molecular Oncology, Cancer Institute, CAMS, PUMC, Beijing 100021, China.
authors.forename: C
authors.initials: C
authors.lastname: Lin
authors.forename: Y
authors.initials: Y
authors.lastname: Deng
authors.forename: J
authors.initials: J
authors.lastname: Zheng
authors.forename: M
authors.initials: M
authors.lastname: Fu
authors.forename: J P
authors.initials: JP
authors.lastname: Chen
authors.forename: P
authors.initials: P
authors.lastname: Xiao
authors.forename: M
authors.initials: M
authors.lastname: Wu
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D002584:Cervix Uteri / Q000166:cytology*; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects*; D005260:Female; D016193:G1 Phase; D016195:G2 Phase; D005786:Gene Expression Regulation; D016259:Genes, myc / Q000235:genetics; D006801:Humans; D008297:Male; D010087:Oxides / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D001152:Arsenicals; D010087:Oxides; D000077237:Arsenic Trioxide