[Gene clone of autoantigen hnRNP A2/B1 and research on expression in synovium].
OBJECTIVE
To investigate hnRNPA2/B1's role in rheumatoid synovitis by obtaining its cDNA sequence and analyzing its expression in synovium.


METHODS
Total RNA was isolated from peripheral blood mononuclear cells (PBMC) and the RT-PCR was performed. The fragment was cloned into PUC-T1 plasmids and further sequenced. We detected it's expression in synovium with monoclonal antibodies and specific cDNA probes by immunohistochemistry and in situ hybridization.


RESULTS
The fragment was identified by DNA sequencing. The expression in rheumatoid synovium was higher than that from osteoarthritis and the normal controls (P < 0.05).


CONCLUSIONS
hnRNP A2/B1 cDNA has been cloned successfully and hnRNP A2/B1 might play an important role in rheumatoid synovitis.
Yang
L
L
Department of Rheumatology, PUMC Hospital, CAMS, PUMC, Beijing 100730, China.
Yu
M X
MX
Lin
J Y
JY
Gao
Y
Y
Chen
J
J
D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D005260:Female; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D013583:Synovial Membrane / Q000378:metabolism*
D018076:DNA, Complementary; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B