Marker-assisted study of genetic background and gene-targeted locus modifiers in lymphopoietic phenotypes.
Forward and reverse genetic approaches facilitate the molecular dissection of individual gene functions and the integration of individual gene functions into multi-gene processes in the context of the whole organism. Variations in mutant phenotypes due to genetic background differences have been well documented through the analysis of mouse mutants. Nevertheless, recommendations concerning the assessment of genetic background as it impacts on phenotype, and utilization of genetic background differences to identify and integrate gene functions have been largely overlooked. Genetic background as it relates to immunological mutants will be discussed utilizing an Ets1-targeted allele to exemplify phenotypic variation due to background. Marker-assisted strategies for the identification of genetic modifiers, especially those linked to the targeted locus, will also be considered.
Spyropoulos
Demetri D
DD
Laboratory of Cancer Genomics, Hollings Cancer Center, Department of Cell Biology and Anatomy, Medical University of South Carolina, 86 Jonathan Lucas St., Charleston, SC 29425, USA. spyropdd@musc.edu
Bartel
Frank O
FO
Higuchi
Tsukasa
T
Deguchi
Takao
T
Ogawa
Makio
M
Watson
Dennis K
DK
D000818:Animals; D003433:Crosses, Genetic; D005819:Genetic Markers / Q000235:genetics*; D006801:Humans; D016130:Immunophenotyping; D038041:Lymphopoiesis / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics*
D005819:Genetic Markers