title: Extension of biochemical functions by the introduction of nonnatural amino acids and artificial nucleic acid analogs.
abstract: Extension of biochemical functions has been attempted by introducing nonatural amino acids and artificial nucleic acid analogs. Nonnatural amino acids have been linked to tRNAs and the amino-acylated tRNAs were added to E. coli in vitro protein synthesizing system to produce nonnatural mutant proteins. The positions of the nonnatural amino acids have been assigned by the 4-base codons, like CGGG and AGGU. The extended codons have been introduced at a specific position or at random positions on a DNA. In the latter case, a DNA library that contains a single 4-base codon at random positions can be obtained. The combination of these new techniques opens a way to the introduction of artificial functions to biochemical systems.
authors.affiliation: Department of Bioscience and Biotechnology, Okayama University, 3-1-1 Tsushimanaka, Okayama 700-8530, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Sisido
mesh_terms: D000596:Amino Acids / Q000737:chemistry* / Q000378:metabolism*; D001483:Base Sequence; D003062:Codon / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005456:Fluorescent Dyes; D016296:Mutagenesis; D016254:Mutagenesis, Insertional; D009696:Nucleic Acids / Q000737:chemistry* / Q000378:metabolism*; D020135:Peptide Nucleic Acids / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D000596:Amino Acids; D003062:Codon; D005456:Fluorescent Dyes; D009696:Nucleic Acids; D020135:Peptide Nucleic Acids; D011994:Recombinant Proteins