Synthesis and physicochemical study of the laminin active sequence: SIKVAV.
The synthesis, physicochemical characterization, and interaction with membrane model systems of a peptide derived from the PA22-2 region of laminin are described. Surface activity studies indicate that this peptide is able to spread at the air-water interface being the maximal spreading pressure 20 mN/m at subphase concentrations around 10 micro M. Besides, these peptide molecules are also able to form stable monolayers. Physicochemical studies concerning the interaction of this peptide with lipids, organized in mono and bilayers, were carried out using Langmuir balance experiments and polarization fluorescence techniques. The peptide penetrates better in monolayers of DPPC than in those of PC and forms condensed mixed monolayers with DPPC. Energies of mixing are small thus indicating that deviations from ideality were almost negligible. Interactions with bilayers were studied through microviscosity changes (DPH and TMA-DPH probes), membrane permeability alterations (CF, NBD-PE/dithionite), and fusion promotion (NBD-PE/Rh-PE, resonance energy transfer). Results indicate that this sequence interacts very softly with bilayers without promoting changes in their organization. These data as well as the lack of interaction with erythrocytes suggest that coating liposomes with this peptide through chemical amide bonds can render stable inmunoliposomes for further biological applications.
Almiñana
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Department of Peptides, Institute for Chemical and Environmental Research, CSIC, Jordi Girona 18-26, 08034, Barcelona, Spain. fripfr@iiqab.csic.es
Alsina
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MA
Espina
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M
Reig
F
F
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D000388:Air; D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D007700:Kinetics; D007797:Laminin / Q000138:chemical synthesis* / Q000737:chemistry / Q000502:physiology*; D008051:Lipid Bilayers / Q000737:chemistry; D008055:Lipids / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D015233:Models, Statistical; D010452:Peptide Biosynthesis; D010455:Peptides / Q000737:chemistry; D011312:Pressure; D011817:Rabbits; D013696:Temperature; D013997:Time Factors; D014867:Water / Q000737:chemistry
D007797:Laminin; D008051:Lipid Bilayers; D008055:Lipids; D008081:Liposomes; D010455:Peptides; D014867:Water; D015060:1,2-Dipalmitoylphosphatidylcholine