Restoration of gap junctional intercellular communication by dibutyryl-cAMP in renal epithelial cells treated with renal carcinogen.
Dimethylnitrosamine(DMN) is an alkylating agent and a known renal carcinogen. A short exposure of renal epithelial cells to cytotoxic concentrations of DMN influences the expression of gap junction proteins. In this study, we examined gap junctional intercellular communication and connexin 43 expression in renal epithelial cells treated with 1% DMN and also examined the effects of dibutyryl-cAMP on preventing gap junctional disturbances. Connexin 43 becomes hypophosphorylated after treatment with 1% DMN for 15 minutes, but this hypophosphorylation is inhibited by pretreatment with dibutyryl-cAMP. These results suggest that changes in gap junction protein expression are early events associated with 1% DMN treatment of renal epithelial cells, and such changes are prevented by dibutyryl-cAMP pretreatment.
Sato
Hidesuke
H
Department of Urology, Nagasaki University School of Medicine, Sakamoto 1-7-1, Nagasaki, Japan 852-8501.
Nomata
Koichiro
K
Noguchi
Mitsuru
M
Takahashi
Hajime
H
Watanabe
Jun-Ichi
J
Kanetake
Hiroshi
H
D000818:Animals; D015153:Blotting, Western; D003994:Bucladesine / Q000494:pharmacology*; D002273:Carcinogens / Q000037:antagonists & inhibitors* / Q000633:toxicity; D002450:Cell Communication / Q000187:drug effects* / Q000502:physiology; D002463:Cell Membrane Permeability / Q000187:drug effects / Q000502:physiology; D018031:Connexin 43 / Q000096:biosynthesis / Q000378:metabolism; D004128:Dimethylnitrosamine / Q000037:antagonists & inhibitors* / Q000633:toxicity; D004285:Dogs; D004347:Drug Interactions; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D017629:Gap Junctions / Q000187:drug effects* / Q000502:physiology; D007668:Kidney / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D010766:Phosphorylation / Q000187:drug effects
D002273:Carcinogens; D018031:Connexin 43; D003994:Bucladesine; D007770:L-Lactate Dehydrogenase; D004128:Dimethylnitrosamine