title: Histone acetyltransferase (HAT) activity of ATF-2 is necessary for the CRE-dependent transcription.
abstract: ATF-2 is a DNA-binding protein that binds to cAMP-response elements (CREs) and forms a hetrodimer with c-Jun, via binding of the leucine zipper motif and then stimulates the CRE-dependent transcription (1,2). Recently, we have reported that ATF-2 has an intrinsic acetyltransferase activity that is controlled by phosphorylation. Mutant form of either p300 or ATF-2 with mutations in the HAT domain failed to stimulate the CRE-dependent transcription in response to UV irradiation. Moreover, phosphorylation of ATF-2 enhanced its HAT activity and CRE-dependent transcription. Thus, these results indicate that HAT activity of ATF-2 is important for the CRE-dependent transcription.
authors.affiliation: Tsukuba Life Science Center, Institute of Physical and Chemical Research (RIKEN), National Institute for Advanced Interdisciplinary Research (NAIR), Tsukuba Science City, Ibaraki, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kawasaki
authors.forename: K
authors.initials: K
authors.lastname: Taira
authors.forename: K
authors.initials: K
authors.lastname: Yokoyama
mesh_terms: D000123:Acetyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051698:Activating Transcription Factor 2; D017362:Cyclic AMP Response Element-Binding Protein / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016758:Genes, jun; D006367:HeLa Cells; D051548:Histone Acetyltransferases; D006801:Humans; D008156:Luciferases / Q000235:genetics; D016297:Mutagenesis, Site-Directed; D009687:Nuclear Proteins / Q000378:metabolism; D010766:Phosphorylation; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015534:Trans-Activators / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D014466:Ultraviolet Rays
chemical_list: C497105:ATF2 protein, human; D051698:Activating Transcription Factor 2; D017362:Cyclic AMP Response Element-Binding Protein; D009687:Nuclear Proteins; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D008156:Luciferases; D000123:Acetyltransferases; D051548:Histone Acetyltransferases