Characterization of Anaplasma marginale isolated from North American bison.
Anaplasma marginale (Rickettsiales: Anaplasmataceae), a tick-borne pathogen of cattle, is endemic in tropical and subtropical regions of the world. Although serologic tests have identified American bison, Bison bison, as being infected with A. marginale, the present study was undertaken to confirm A. marginale infection and to characterize isolates obtained from naturally infected bison in the United States and Canada. Major surface protein (MSP1a and MSP4) sequences of bison isolates were characterized in comparison with New World cattle isolates. Blood from one U.S. bison was inoculated into a susceptible, splenectomized calf, which developed acute anaplasmosis, demonstrating infectivity of this A. marginale bison isolate for cattle. The results of this study showed that these A. marginale isolates obtained from bison were similar to ones from naturally infected cattle.
De La Fuente
José
J
Department of Veterinary Pathobiology, College of Veterinary Medicine, Stillwater, Oklahoma 74078, USA. jose_delafuente@yahoo.com
Golsteyn Thomas
Elizabeth J
EJ
van den Bussche
Ronald A
RA
Hamilton
Robert G
RG
Tanaka
Elaine E
EE
Druhan
Susan E
SE
Kocan
Katherine M
KM
D000595:Amino Acid Sequence; D000709:Anaplasma / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D016164:Bison / Q000382:microbiology*; D002417:Cattle / Q000382:microbiology; D005260:Female; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny
D001426:Bacterial Proteins; D008565:Membrane Proteins; C085498:Msp4 protein, Anaplasma marginale