[Construction of a collagen gel dermis with improved biological property].
OBJECTIVE
To improve the biological property of artificial skin.


METHODS
We have ameliorated Hansburgh and Middelkoop's method of manufacturing artificial dermis. The type I collagenase and Dispase were used to isolated neonate prepuce' dermis fibroblast. The gel dermis was constructed by compounding the fibroblast and collagen swelling solution. The property of the collagen gel dermis was measured.


RESULTS
The neonate prepuce's dermis fibroblast had property of high proliferation, high activation of the dermis, and it could secrete abundant extracellular matrix (ECM).


CONCLUSIONS
The collagen gel dermis is an useful dermis substitute.
Liu
Ling-rong
LR
Department of Biological Material, Institute of Biomedical Engineering, CAMS, PUMC, Tianjin 300192, China.
Zhang
Li-hai
LH
Ma
Dong-rui
DR
Zhang
Qi-qing
QQ
D002469:Cell Separation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis; D020405:Dermis / Q000166:cytology / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D017768:Skin, Artificial; D023822:Tissue Engineering
D024042:Collagen Type I; D016326:Extracellular Matrix Proteins