Genome-wide insertional mutagenesis of Arabidopsis thaliana.
Over 225,000 independent Agrobacterium transferred DNA (T-DNA) insertion events in the genome of the reference plant Arabidopsis thaliana have been created that represent near saturation of the gene space. The precise locations were determined for more than 88,000 T-DNA insertions, which resulted in the identification of mutations in more than 21,700 of the approximately 29,454 predicted Arabidopsis genes. Genome-wide analysis of the distribution of integration events revealed the existence of a large integration site bias at both the chromosome and gene levels. Insertion mutations were identified in genes that are regulated in response to the plant hormone ethylene.
Alonso
José M
JM
Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
Stepanova
Anna N
AN
Leisse
Thomas J
TJ
Kim
Christopher J
CJ
Chen
Huaming
H
Shinn
Paul
P
Stevenson
Denise K
DK
Zimmerman
Justin
J
Barajas
Pascual
P
Cheuk
Rosa
R
Gadrinab
Carmelita
C
Heller
Collen
C
Jeske
Albert
A
Koesema
Eric
E
Meyers
Cristina C
CC
Parker
Holly
H
Prednis
Lance
L
Ansari
Yasser
Y
Choy
Nathan
N
Deen
Hashim
H
Geralt
Michael
M
Hazari
Nisha
N
Hom
Emily
E
Karnes
Meagan
M
Mulholland
Celene
C
Ndubaku
Ral
R
Schmidt
Ian
I
Guzman
Plinio
P
Aguilar-Henonin
Laura
L
Schmid
Markus
M
Weigel
Detlef
D
Carter
David E
DE
Marchand
Trudy
T
Risseeuw
Eddy
E
Brogden
Debra
D
Zeko
Albana
A
Crosby
William L
WL
Berry
Charles C
CC
Ecker
Joseph R
JR
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000483:Alleles; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D001482:Base Composition; D032461:Chromosomes, Plant / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D005030:Ethylenes / Q000494:pharmacology; D005091:Exons; D020224:Expressed Sequence Tags; D015870:Gene Expression; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D018745:Genome, Plant; D007438:Introns; D016254:Mutagenesis, Insertional; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D012231:Rhizobium / Q000235:genetics
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D029681:Arabidopsis Proteins; D004269:DNA, Bacterial; D018744:DNA, Plant; D005030:Ethylenes; C036483:T-DNA; C036216:ethylene