title: Avian Purkinje neuronal cultures: extrinsic control of morphology by cell type and glutamate.
abstract: An in vitro coculture system is described to study the avian Purkinje neuron and the interactions occurring with astrocytes and granule cells during development in the cerebellum. Astrocytes initially and granule cells later regulate Purkinje neuron morphology. The coculture system presented here provides an excellent system for investigating the morphological, immunocytochemical, and electrophysiological differentiation of Purkinje neurons under controlled conditions and for studying cell-cell interactions and extrinsic factors, e.g., glutamate in normal and neuropathological conditions.
authors.affiliation: Developmental Neurobiology Group, Children's Medical Research Institute, Westmead, New South Wales 2145, Australia.
authors.forename: Peter L
authors.initials: PL
authors.lastname: Jeffrey
authors.forename: Vladimir J
authors.initials: VJ
authors.lastname: Balcar
authors.forename: Ornella
authors.initials: O
authors.lastname: Tolhurst
authors.forename: Ron P
authors.initials: RP
authors.lastname: Weinberger
authors.forename: Jenny A
authors.initials: JA
authors.lastname: Meany
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D002450:Cell Communication / Q000187:drug effects / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D002642:Chick Embryo; D018920:Coculture Techniques / Q000295:instrumentation / Q000379:methods; D027342:Excitatory Amino Acid Transporter 2 / Q000037:antagonists & inhibitors / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism / Q000494:pharmacology*; D011689:Purkinje Cells / Q000166:cytology* / Q000187:drug effects* / Q000502:physiology
chemical_list: D027342:Excitatory Amino Acid Transporter 2; D018698:Glutamic Acid