[Short-term effects of cyclosporine A on the function of pancreatic islet cells in normal and spontaneous diabetic Chinese hamsters].
OBJECTIVE
To examine the effects of low dose (25-100 ng/ml) CsA on the hormonal secretion of the pancreatic islet cells isolated from normal and spontaneously inherited diabetic Chinese hamsters.


METHODS
A modified kanatsuna and Knudsen's column perifusion system was developed. The secretions of Insulin (Ins) and glucagon (Glc) of the cultured islet cells responding to the glucose stimulations were tested after pretreated with different doses of CsA for 24 hours.


RESULTS
The secretions of Ins of the diabetic islet cells with and without glucose stimulations (16.7 mmol/L) both reduced significantly compared to the normal controls (P < 0.05). In contrast, the static secretions of Glc increased significantly in the diabetes islet cells (P < 0.05). The response (Ins release) of normal islets to glucose stimuli was significantly inhibited after 24-hour pretreatment with CsA (25-100 ng/ml, P < 0.05), while the concentrations of Ins and Glc detected in the culture fluids did not differ from the controls (P > 0.05).


CONCLUSIONS
Abnormal islet function exists in diabetic hamsters characterized by impairment of Ins secretion and increase of Glc secretion. Low dose CsA has no significant effects on the secretions of Ins and Glc, but it inhibits the response of the islets to the glucose stimuli.
Wang
Yu
Y
Department of Endocrinology, West China Hospital, Sichuan University, Chengdu 610041, China.
Li
Xiujun
X
Zhao
Guizhi
G
Deng
Shangping
S
D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D016572:Cyclosporine / Q000494:pharmacology*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005934:Glucagon / Q000378:metabolism; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism*
D007166:Immunosuppressive Agents; D007328:Insulin; D016572:Cyclosporine; D005934:Glucagon