Viability of microencapsulated bifidobacteria in simulated gastric juice and bile solution.
Microencapsulated cells of Bifidobacterium longum B6 and Bifidobacterium infantis CCRC 14633 were prepared by spray drying the cell suspension containing the test organism and 10% (w/w) of the carrier material of either gelatin, soluble starch, skim milk or gum arabic. Survival of these microencapsulated and free cells of bifidobacteria in simulated gastric juice (pH 2.0 and 3.0) and bile solution (0.5% and 2.0%) was then examined. B. infantis CCRC 14633 was more susceptible than B. longum B6 to the simulated gastric environment and bile solution tested. Microencapsulated bifidobacteria exhibited a lower population reduction than free cells during exposure to simulated gastric environment and bile solution. This phenomenon was most pronounced when the test organism was exposed to gastric juice at pH 2.0 or 2.0% bile solution. Moreover, it was also observed that the protective effect exerted by encapsulation with spray drying varied with the carriers used and the strains of bifidobacteria.
Lian
Wen-Chian
WC
Graduate Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan, ROC.
Hsiao
Hung-Chi
HC
Chou
Cheng-Chun
CC
D000818:Animals; D001644:Bifidobacterium / Q000187:drug effects / Q000254:growth & development*; D001647:Bile Acids and Salts / Q000494:pharmacology*; D015169:Colony Count, Microbial; D004339:Drug Compounding; D005516:Food Microbiology; D005750:Gastric Juice / Q000382:microbiology; D005780:Gelatin / Q000494:pharmacology; D006170:Gum Arabic / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008892:Milk / Q000382:microbiology; D008954:Models, Biological; D012995:Solubility; D013213:Starch / Q000494:pharmacology
D001647:Bile Acids and Salts; D006170:Gum Arabic; D005780:Gelatin; D013213:Starch