[Continuous monitoring of blood adriamycin using a fiber optic chemical sensor in rabbit].
OBJECTIVE
To evaluate the feasibility and accuracy of continuous monitoring of drug with a fiber optic chemical sensor (FOCS) in animal.


METHODS
An accurate optical design was used to enhance the intensity of light from a 100-micron optic fiber and the fluorescence signal could be detected. A new sol-gel method was used to fix the fluorescence substance 4-(N, N-dioctyl) amino-7-nitrobenz-2-oxa-1, 3-diazole (D-70) on the body fiber. The vary quenching means the vary of the concentration of adriamycin (ADM) in rabbit blood. ADM was determined by FOCS based on the fluorescence multiple quenching. In a simple animal model, the carotid artery was catheterized with a cannula, housing a 100-micron optic fiber.


RESULTS
The recovery of ADM was 99.4%-106.2%, the within-run and between-run RSDs were 6.6%-11.4% and 5.9%-11.7% respectively. The method permitted detection limits as low as 0.057 microgram.mL-1 at a signal-to-noise ratio of 3.


CONCLUSIONS
Fiber-optic chemical sensor is potentially useful for monitoring blood drug in biomedical field.
Lu
Wen-xu
WX
Department of Pharmacy, Tumor Hospital, Urumqi, China. lvwenxu@netease.com
Chen
Jian
J
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000097:blood*; D019540:Area Under Curve; D004317:Doxorubicin / Q000097:blood* / Q000493:pharmacokinetics; D016903:Drug Monitoring / Q000295:instrumentation / Q000379:methods*; D005336:Fiber Optic Technology / Q000379:methods*; D005453:Fluorescence; D055100:Optical Fibers; D011817:Rabbits; D014159:Transducers
D000903:Antibiotics, Antineoplastic; D004317:Doxorubicin