title: Identification of Plasmodium falciparum antigens by antigenic analysis of genomic and proteomic data.
abstract: The recent explosion in genomic sequencing has made available a wealth of data that can now be analyzed to identify protein antigens, potential targets for vaccine development. Here we present, in the context of Plasmodium falciparum, a strategy that rapidly identifies target antigens from large and complex genomes. Sixteen antigenic proteins recognized by volunteers immunized with radiation-attenuated P. falciparum sporozoites, but not by mock immunized controls, were identified. Several of these were more antigenic than previously identified and well characterized P. falciparum-derived protein antigens. The data suggest that immune responses to Plasmodium are dispersed on a relatively large number of parasite antigens. These studies have implications for our understanding of immunodominance and breadth of responses to complex pathogens.
authors.affiliation: Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910-7500, USA.
authors.forename: Denise L
authors.initials: DL
authors.lastname: Doolan
authors.forename: Scott
authors.initials: S
authors.lastname: Southwood
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Freilich
authors.forename: John
authors.initials: J
authors.lastname: Sidney
authors.forename: Norma L
authors.initials: NL
authors.lastname: Graber
authors.forename: Lori
authors.initials: L
authors.lastname: Shatney
authors.forename: Lolita
authors.initials: L
authors.lastname: Bebris
authors.forename: Laurence
authors.initials: L
authors.lastname: Florens
authors.forename: Carlota
authors.initials: C
authors.lastname: Dobano
authors.forename: Adam A
authors.initials: AA
authors.lastname: Witney
authors.forename: Ettore
authors.initials: E
authors.lastname: Appella
authors.forename: Stephen L
authors.initials: SL
authors.lastname: Hoffman
authors.forename: John R
authors.initials: JR
authors.lastname: Yates
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Carucci
authors.forename: Alessandro
authors.initials: A
authors.lastname: Sette
mesh_terms: D000328:Adult; D000465:Algorithms; D000483:Alleles; D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics* / Q000302:isolation & purification; D017125:Genes, Protozoan; D018503:Genome, Protozoan; D023281:Genomics; D006801:Humans; D007114:Immunization; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000096:biosynthesis; D017780:Malaria Vaccines / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000235:genetics* / Q000276:immunology*; D040901:Proteomics; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000953:Antigens, Protozoan; D017780:Malaria Vaccines; D015800:Protozoan Proteins; D007371:Interferon-gamma