Preparation and enzymatic recognition of guanine lesions induced by nitrogen oxides.
Xanthine (Xan) and oxanine (Oxa) are the major deamination products of guanine formed by the treatment with nitrogen oxides (e.g., NO and HNO2). In this study, 2'-deoxyribonucleoside 5'-triphosphates of Xan and Oxa were prepared by the NaNO2 treatment of dGTP. These modified nucleotides were incorporated into oligonucleotides by DNA polymerase reactions. The repair activities of various DNA N-glycosylases for Xan and Oxa were examined using these substrates.
Masaoka
A
A
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima 739-8526, Japan.
Terato
H
H
Honsho
A
A
Ohyama
Y
Y
Suzuki
T
T
Yamada
M
M
Makino
K
K
Ide
H
H
D004249:DNA Damage; D045647:DNA Glycosylases; D004260:DNA Repair; D003854:Deoxyribonucleotides / Q000378:metabolism; D006147:Guanine / Q000378:metabolism*; D066298:In Vitro Techniques; D009699:N-Glycosyl Hydrolases / Q000378:metabolism; D009569:Nitric Oxide / Q000633:toxicity*; D009585:Nitrogen Dioxide / Q000633:toxicity*; D009711:Nucleotides / Q000378:metabolism; D011684:Purine Nucleosides / Q000378:metabolism; D011758:Pyrroles / Q000378:metabolism; D019820:Xanthine / Q000378:metabolism
C110411:1-(2'-deoxyribofuranosyl)-3-nitropyrrole triphosphate; D003854:Deoxyribonucleotides; D009711:Nucleotides; D011684:Purine Nucleosides; D011758:Pyrroles; C468766:oxanine; D019820:Xanthine; D009569:Nitric Oxide; D006147:Guanine; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D009585:Nitrogen Dioxide