Pro-oxidative activity of nitroxides in their reactions with glutathione.
Nitroxides are unreactive towards glutathione in vitro. Interaction of nitroxides with peroxynitrite does not lead to a significant loss of their electron paramagnetic resonance (EPR) signal. However, addition of peroxynitrite to a solution containing glutathione and nitroxides induces an irreversible disappearance of EPR signal of nitroxides and augmentation of glutathione oxidation which is a pro-oxidant effect of these compounds. Nitroxide loss leading to the formation of amine derivatives is initiated by products of glutathione oxidation by peroxynitrite. The pro-oxidant action of nitroxides at micromolar concentrations may be important in view of the proposed use of these compounds as antioxidants.
Głebska
Jolanta
J
Department of Molecular Biophysics, University of Łodź, Poland.
Skolimowski
Janusz
J
Kudzin
Zbigniew
Z
Gwoździński
Krzysztof
K
Grzelak
Agnieszka
A
Bartosz
Grzegorz
G
D004305:Dose-Response Relationship, Drug; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D005978:Glutathione / Q000737:chemistry / Q000378:metabolism*; D013058:Mass Spectrometry; D008956:Models, Chemical; D009569:Nitric Oxide; D016877:Oxidants / Q000737:chemistry / Q000494:pharmacology*; D030421:Peroxynitrous Acid / Q000138:chemical synthesis; D013997:Time Factors
D005609:Free Radicals; D016877:Oxidants; D030421:Peroxynitrous Acid; D009569:Nitric Oxide; D005978:Glutathione