[The induction of neuronal differentiation in the glial fibrillary acid protein positive human neural progenitor cell line].
OBJECTIVE
To investigate the ability of human GFAP positive neural progenitor cell line from the subventricular zone (SVZ) to differentiate into neurons.


METHODS
Real-time RT-PCR, Western blot analysis and immunocytochemistry were used to examine the expression level of the neural stem cell marker and neuronal-specific marker before and after all-trans-retinoic acid (AT-RA) induction in the GFAP positive neural progenitor cell line. Immunocytochemistry was used to examine the expression of the neuronal-specific marker after transplantation the GFAP positive neural progenitor cell line into the animal model.


RESULTS
After induction, in the GFAP positive neural progenitor cell line the expression levels of the neuronal-specific marker increased, while the neural stem cell marker decreased both in mRNA and protein levels. After transplantation into animal model, the GFAP positive neural progenitor cell line could differentiate into neurons.


CONCLUSIONS
The GFAP positive neural progenitor cell line could be induced to differentiate into neurons both in vitro and in vivo.
Bai
Yun
Y
Peking University Stem Cell Research Center, Beijing 100083, China.
Lin
Changsheng
C
Hu
Qikuan
Q
Li
Xiaoxia
X
Lu
Aili
A
Wang
Shuling
S
Li
Lingsong
L
Shen
Li
L
D002454:Cell Differentiation; D002460:Cell Line; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis* / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000032:analysis / Q000235:genetics; D009419:Nerve Tissue Proteins; D064231:Nestin; D009474:Neurons / Q000737:chemistry / Q000166:cytology*; D010749:Phosphoprotein Phosphatases / Q000032:analysis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D013234:Stem Cells / Q000737:chemistry / Q000166:cytology*
D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin; D012333:RNA, Messenger; C049301:microtubule-associated protein-2 phosphatase; D010749:Phosphoprotein Phosphatases