BHK cell attachment and growth on EDA-plasma-modified poly(L-lactide/epsilon-caprolactone) biodegradable films.
In this study, attachment and growth of Baby Hamster Kidney (BHK) cells on ethylene diamine (EDA)-plasma-treated poly(L-lactide/epsilon-caprolactone) biodegradable copolymer films were investigated. The co-polymer (Mw: 58000; Mn: 35000 and PI 1.60) was synthesised by ring-opening polymerization of the respective dimers with using stannous octoate as the catalyst. The final ratio of L-lactide to epsilon-caprolactone obtained by 1H-NMR was 87:13. The co-polymer films were treated with the EDA-plasma in a glow-discharge apparatus. The BHK-30 cell line was cultured on plain and EDA-plasma-treated films and their pre-wetted forms (with ethanol and/or cell culture medium before use). Cell attachment and growth were followed. Alkaline phosphatase (ALP) activity and glucose uptake in cell culture medium were also investigated. There was no attachment in the first 12 h. Glow-discharge treatment increased significantly the attachment and growth. Pre-wetting with ethanol and cell culture medium was also increase significantly both the attachment and growth.
Gürpinar
O Aylin
OA
Hacettepe University, Faculty of Science, Department of Biology, Beytepe, 06532 Ankara, Turkey.
Tuzlakoğlu
Kadriye
K
Onur
M Ali
MA
Tümer
Aşkin
A
Serdar
Muhittin A
MA
Unal
Nilay
N
Pişkin
Erhan
E
D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry*; D002448:Cell Adhesion; D002455:Cell Division; D002460:Cell Line; D006224:Cricetinae; D003470:Culture Media; D004847:Epithelial Cells / Q000378:metabolism*; D000431:Ethanol / Q000737:chemistry; D005029:Ethylenediamines / Q000737:chemistry*; D005947:Glucose / Q000493:pharmacokinetics; D007526:Isoelectric Point; D007668:Kidney / Q000166:cytology; D007783:Lactones / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008567:Membranes, Artificial; D008970:Molecular Weight; D011091:Polyesters / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D013997:Time Factors
D001672:Biocompatible Materials; D003470:Culture Media; D005029:Ethylenediamines; D007783:Lactones; D008567:Membranes, Artificial; D011091:Polyesters; D011108:Polymers; D000431:Ethanol; C031234:ethylenediamine; D000469:Alkaline Phosphatase; D005947:Glucose