[Detection of ermB gene responsible for high level resistance to clindamycin (MLS type resistance) among Clostridium difficile strains isolated from antibiotic associated diarrhea (AAD)].
In 68 C. difficile strains isolated from feacal samples of patients with antibiotic associated diarrhoea (AAD) investigated presence of ermB gene transferable of high level resistance to clindamycin. The primers set 2980/2981 used for identification of ermB gene amplified a 688 bp segment. We used the Etest to assess all strains for susceptibility to clindamycin. This study demonstrates that 57% of strains isolated from faecal samples of patients with AAD were highly resistant to clindamycin (minimal inhibitory concentration (MIC) of clindamycin, 256 mg/L) and possessed the ermB gene.
Pituch
Hanna
H
Katedra i Zakład Mikrobiologii Lekarskiej AM w Warszawie.
Obuch-Woszczatyński
Piotr
P
Meisel-Mikołajczyk
Felicja
F
Łuczak
Miroslaw
M
D002981:Clindamycin / Q000009:adverse effects / Q000494:pharmacology*; D016360:Clostridioides difficile / Q000187:drug effects* / Q000235:genetics*; D003967:Diarrhea / Q000139:chemically induced / Q000382:microbiology; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004761:Enterocolitis, Pseudomembranous / Q000139:chemically induced / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D006801:Humans; D008780:Methyltransferases / Q000302:isolation & purification*; D008826:Microbial Sensitivity Tests
D002981:Clindamycin; D008780:Methyltransferases; C035181:rRNA (adenosine-O-2'-)methyltransferase