Cloning and characterization of the fur gene from Moraxella bovis.
A homologue of the ferric uptake regulator gene (fur) was isolated from Moraxella bovis by degenerate polymerase chain reaction and cloning. Fur protein of M. bovis exhibited 72.1% amino acid identity with Acinetobacter calcoaceticus Fur. Western blot analysis showed a decrease of Fur expression in response to sufficient-iron conditions compared with deficient-iron conditions. An electrophoretic mobility-shift assay indicated that Fur protein binds to DNA fragments containing a putative Fur-box derived from the upstream region of the M. bovis fur gene. Fur of M. bovis may regulate the expression of iron transport systems in response to iron limitation in the environment.
Kakuda
Tsutomu
T
Department of Animal Hygiene, School of Veterinary Medicine and Animal Science, Kitasato University, Towada, Aomori 034-8628, Japan. kakuda@vmas.kitasato-u.ac.jp
Oishi
Daiki
D
Tsubaki
Shiro
S
Takai
Shinji
S
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D007501:Iron / Q000494:pharmacology; D008969:Molecular Sequence Data; D016955:Moraxella bovis / Q000187:drug effects / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D012097:Repressor Proteins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D018076:DNA, Complementary; D012097:Repressor Proteins; C068477:ferric uptake regulating proteins, bacterial; D007501:Iron