title: Differential induction of protein kinase C isoforms at the cardiac hypertrophy stage and congestive heart failure stage in Dahl salt-sensitive rats.
abstract: Several protein kinase C (PKC) isoforms may play important roles in cellular signaling pathways. Recent reports have suggested that PKC plays critical isoform-specific roles in the development of cardiac hypertrophy and heart failure. The purpose of the present study was to examine the expression profiles of PKC isoforms in models of cardiac hypertrophy and heart failure. We examined the cardiac expression of individual PKC isoforms at the cardiac hypertrophy stage and the heart failure stage in Dahl salt-sensitive rats by Western blot analysis. The levels of all PKC isoforms increased at the cardiac hypertrophy stage and the heart failure stage, but the pattern of increase differed among PKC isoforms at the heart failure stage. The expressions of PKCalpha, beta, and delta increased at the cardiac hypertrophy stage and remained elevated at the heart failure stage. On the other hand, the expression of PKCepsilon and atypical PKCs (aPKCs) increased at the cardiac hypertrophy stage, but this increase tended to decline at the congestive heart failure stage. These results suggest that there are two groups of PKC isoforms. Several reports have shown that PKCalpha, beta, and delta are involved in the development of cardiac hypertrophy and heart failure, and that PKCepsilon plays a role in the physiological hypertrophic responses and cardioprotective actions. These facts suggest that all PKC isoforms (PKCalpha, beta, delta, epsilon, and aPKCs) expressed in the heart may have similar functions at the cardiac hypertrophy stage, but that two groups of PKC isoforms (PKCalpha, beta, delta, and PKCepsilon, aPKCs) have different functions at the congestive heart failure stage.
authors.affiliation: Department of Medicine II, Yokohama City University School of Medicine, Yokohama, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Koide
authors.forename: Kouichi
authors.initials: K
authors.lastname: Tamura
authors.forename: Atsushi
authors.initials: A
authors.lastname: Suzuki
authors.forename: Kouichi
authors.initials: K
authors.lastname: Kitamura
authors.forename: Keiko
authors.initials: K
authors.lastname: Yokoyama
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Hashimoto
authors.forename: Nobuhito
authors.initials: N
authors.lastname: Hirawa
authors.forename: Minoru
authors.initials: M
authors.lastname: Kihara
authors.forename: Shigeo
authors.initials: S
authors.lastname: Ohno
authors.forename: Satoshi
authors.initials: S
authors.lastname: Umemura
mesh_terms: D000818:Animals; D006332:Cardiomegaly / Q000378:metabolism*; D006333:Heart Failure / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000201:enzymology; D011493:Protein Kinase C / Q000378:metabolism*; D064546:Protein Kinase C beta; D051571:Protein Kinase C-alpha; D051745:Protein Kinase C-delta; D051744:Protein Kinase C-epsilon; D051381:Rats; D020303:Rats, Inbred Dahl; D015398:Signal Transduction / Q000502:physiology; D015854:Up-Regulation / Q000502:physiology
chemical_list: D007527:Isoenzymes; C497260:Prkcd protein, rat; C497257:Prkce protein, rat; D011493:Protein Kinase C; D064546:Protein Kinase C beta; D051571:Protein Kinase C-alpha; D051745:Protein Kinase C-delta; D051744:Protein Kinase C-epsilon