The genome of bacteriophage phiKMV, a T7-like virus infecting Pseudomonas aeruginosa.
The complete DNA sequence of a new lytic T7-like bacteriophage phiKMV is presented. It is the first genome sequence of a member of the Podoviridae that infects Pseudomonas aeruginosa. The linear G + C-rich (62.3%) double-stranded DNA genome of 42,519 bp has direct terminal repeats of 414 bp and contains 48 open reading frames that are all transcribed from the same strand. Despite absence of homology at the DNA level, 11 of the 48 phiKMV-encoded putative proteins show sequence similarity to known T7-type phage proteins. Eighteen open reading frame products have been assigned, including an RNA polymerase, proteins involved in DNA replication, as well as structural, phage maturation, and lysis proteins. Surprisingly, the major capsid protein completely lacks sequence homology to any known protein. Also, the strong virulence toward many clinical P. aeruginosa isolates and a short replication time make phiKMV attractive for phage therapy or a potential source for antimicrobial proteins.
Lavigne
Rob
R
Laboratory of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, Leuven, B-3001, Belgium.
Burkal'tseva
Maria V
MV
Robben
Johan
J
Sykilinda
Nina N
NN
Kurochkina
Lidia P
LP
Grymonprez
Barbara
B
Jonckx
Bart
B
Krylov
Victor N
VN
Mesyanzhinov
Vadim V
VV
Volckaert
Guido
G
D000595:Amino Acid Sequence; D017123:Bacteriophage T7 / Q000235:genetics; D001482:Base Composition; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon / Q000235:genetics; D004261:DNA Replication / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D019143:Evolution, Molecular; D005814:Genes, Viral / Q000235:genetics; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009113:Muramidase / Q000235:genetics; D010802:Phylogeny; D017902:Podoviridae / Q000201:enzymology / Q000235:genetics*; D011550:Pseudomonas aeruginosa / Q000821:virology*; D012343:RNA, Transfer / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D016415:Sequence Alignment; D019065:Virus Assembly / Q000235:genetics
D003062:Codon; D012343:RNA, Transfer; D012321:DNA-Directed RNA Polymerases; D009113:Muramidase