[Model of B immunoblastic lymphomas in the Hu-PBL-SCID mice].
OBJECTIVE
To constitute a model of B immunoblastic lymphomas in the Hu-PBL-SCID mice.


METHODS
The SCID mice were reconstituted by intraperitoneal injection (i.p.) of 5 x 10(7) human lymphocytes from Epstein-Barr virus (EBV) seronegative individuals. After one week, the SCID mice were inoculated with EBV by i.p. injection, and subjected to the investigation of whether there was any tumor in the abdomen of such SCID mice four weeks later. The characteristics of the found tumor was observed by the methods of Hematoxylin-eosin (HE) stain, immunohistochemical staining and polymerase chain reaction (PCR).


RESULTS
Compared with the control groups, all the EBV-infected Hu-PBL-SCID mice had abdominal solid tumors [(32 +/- 12.5) mm3] developed, often located in the liver. HE staining and immunohistochemical staining showed the tumors were human B cell lymphomas. EBV DNA could be detected in the tumors by the PCR.


CONCLUSIONS
The model of B immunoblastic lymphomas in the Hu-PBL-SCID mice is successfully constituted, and may well be useful to the human tumor immunological study.
Chen
Dong-qing
DQ
Department of Immunology, Institute of Basic Medical Science, CAMS, PUMC, Beijing 100005, China.
Bai
Lian-jun
LJ
Liu
Qing-feng
QF
Cui
Lian-xian
LX
He
Wei
W
Ba
De-nian
DN
D000818:Animals; D004195:Disease Models, Animal; D004854:Herpesvirus 4, Human / Q000502:physiology; D006801:Humans; D016400:Lymphoma, Large-Cell, Immunoblastic; D051379:Mice; D016513:Mice, SCID