Establishment and characterization of two squamous cell carcinoma cell lines (HYVC and HMVC) derived from vulva.
Two cell lines, HYVC and HMVC, were established from cultures of the squamous cell carcinoma removed from the vulva of females of 37 and 46 years old, respectively. These cell lines were very similar in their biological characteristics, such as morphology (polygonal), growth pattern (32-43 hr of population doubling time, 50-25% of plating efficiency), heterotransplantability (1 x 10(7) cells produced squamous cell carcinomas in the nude mice), genetics (75-78 of modal chromosomal number), and producing the tumor markers (SCC and TPA). The HPV-DNA was not detected in either the HYVC or HMVC lines by using L1-PCR methods, however, it was detected in the HYVC using E6/E7-PCR.
Kiguchi
Kazushige
K
Dept. Ob. & Gy., Toyoko Hosp., Sch. Of Med. St. Marianna Univ.
Ishiwata
Isamu
I
Ishiwata
Chieko
C
Iwata
Masanori
M
Ishizuka
Bunpei
B
Yoshikawa
Hiroyuki
H
Tachibana
Toshiaki
T
Hashimoto
Hisashi
H
Ishikawa
Hiroshi
H
D000328:Adult; D000818:Animals; D002272:Carcinoembryonic Antigen / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology* / Q000821:virology; D002455:Cell Division; D005260:Female; D006801:Humans; D007621:Karyotyping; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D027383:Papillomaviridae / Q000302:isolation & purification; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured; D014846:Vulvar Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology* / Q000821:virology
D002272:Carcinoembryonic Antigen