The factor C3 conversion in human complement by smooth Shigella flexneri lipopolysaccharides.
Shigella flexneri rods play an important role in human intestinal infections. In the presented studies we have shown that O-acetyl and glucose residues, substituted in main GalNAc-Rha chains of lipopolysaccharide (LPS) are important for the bactericidal effect of human serum. By dot-blot, immunoblotting and ELISA with immobilized LPS we have shown correlation of C3 fragments deposition and serum resistance. LPSs isolated from a serum-sensitive strain deposited more C3 fragments than LPSs from serum-resistant Shigella flexneri strains.
Fudała
Rafał
R
Institute of Microbiology and Immunology, University of Lódź, Poland.
Doroszkiewicz
Włodzimierz
W
Niedbach
Joanna
J
Gamian
Andrzej
A
Weintraub
Andrej
A
Kaca
Wiesław
W
D001770:Blood Bactericidal Activity / Q000276:immunology; D002240:Carbohydrate Sequence; D003167:Complement Activation / Q000276:immunology*; D003176:Complement C3 / Q000276:immunology* / Q000378:metabolism; D015941:Complement Hemolytic Activity Assay; D004405:Dysentery, Bacillary / Q000097:blood / Q000276:immunology* / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting; D008070:Lipopolysaccharides / Q000276:immunology*; D008969:Molecular Sequence Data; D019081:O Antigens / Q000276:immunology; D012763:Shigella flexneri / Q000276:immunology*
D003176:Complement C3; D008070:Lipopolysaccharides; D019081:O Antigens