Discovery of gene function by expression profiling of the malaria parasite life cycle.
The completion of the genome sequence for Plasmodium falciparum, the species responsible for most malaria human deaths, has the potential to reveal hundreds of new drug targets and proteins involved in pathogenesis. However, only approximately 35% of the genes code for proteins with an identifiable function. The absence of routine genetic tools for studying Plasmodium parasites suggests that this number is unlikely to change quickly if conventional serial methods are used to characterize encoded proteins. Here, we use a high-density oligonucleotide array to generate expression profiles of human and mosquito stages of the malaria parasite's life cycle. Genes with highly correlated levels and temporal patterns of expression were often involved in similar functions or cellular processes.
Le Roch
Karine G
KG
Department of Cell Biology ICND202, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA. leroch@scripps.edu
Zhou
Yingyao
Y
Blair
Peter L
PL
Grainger
Muni
M
Moch
J Kathleen
JK
Haynes
J David
JD
De La Vega
Patricia
P
Holder
Anthony A
AA
Batalov
Serge
S
Carucci
Daniel J
DJ
Winzeler
Elizabeth A
EA
D000818:Animals; D000852:Anopheles / Q000469:parasitology; D002453:Cell Cycle; D002875:Chromosomes / Q000235:genetics; D016000:Cluster Analysis; D004912:Erythrocytes / Q000469:parasitology; D015870:Gene Expression; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D017125:Genes, Protozoan; D006801:Humans; D008018:Life Cycle Stages; D008099:Liver / Q000469:parasitology; D016778:Malaria, Falciparum / Q000469:parasitology; D020411:Oligonucleotide Array Sequence Analysis; D010963:Plasmodium falciparum / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D020543:Proteome; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016053:RNA, Protozoan / Q000235:genetics / Q000378:metabolism; D012469:Salivary Glands / Q000469:parasitology; D034101:Sporozoites / Q000235:genetics / Q000254:growth & development; D014158:Transcription, Genetic
D020543:Proteome; D015800:Protozoan Proteins; D012333:RNA, Messenger; D016053:RNA, Protozoan; C085731:erythrocyte membrane protein 1, Plasmodium falciparum