Bone with a vascular flap induced from fat tissue with the use of rhBMP-2 in rats.
Here we report that successful bone formation with a vascular flap inside a cylindrical mold was induced from fat tissue with the use of recombinant human bone morphogenetic protein-2 in rats. Fat tissue connected to blood vessels was prepared to fit into the mold and implanted intramuscularly into the hind leg in Wistar rats. RhBMP-2 (20 micro g) was applied in a collagen sheet previously placed on the inside surface of the mold. Bone formation was confirmed radiologically and morphologically at 2, 4, and 8 weeks after the surgery. In the control group without rhBMP-2 or the group with ligation of the blood vessels before the implantation, bone formation was not observed. Our success in bone formation having a definite size, shape, and blood supply may lead to a therapeutic approach to effective bone reconstitution. The present study is the first report on bone induction from fat tissue by rhBMP-2 in vivo.
Hosoya
M
M
Oral Surgery, Department of Oral Restitution, Division of Oral Health Sciences, Graduate School, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo, Tokyo 113-8549, Japan.
Maruoka
Y
Y
Oda
M
M
Asahina
I
I
Ichinose
S
S
Omura
K
K
D017667:Adipocytes / Q000473:pathology; D000273:Adipose Tissue / Q000098:blood supply* / Q000637:transplantation; D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D001842:Bone and Bones / Q000000981:diagnostic imaging / Q000473:pathology; D003094:Collagen; D006801:Humans; D008026:Ligation; D008297:Male; D008567:Membranes, Artificial; D018482:Muscle, Skeletal / Q000601:surgery; D010006:Osteoblasts / Q000473:pathology; D010012:Osteogenesis / Q000187:drug effects*; D011859:Radiography; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins; D013524:Surgical Flaps / Q000098:blood supply*; D013848:Thigh / Q000601:surgery; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
C525718:BMP2 protein, human; C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D008567:Membranes, Artificial; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; D003094:Collagen