Experimental studies on morphological changes of microcirculation of DMN-induced liver cirrhosis after normothermic ischemia with charge-coupled device microscope.
OBJECTIVE
The purpose of the present experiment was to find indices for intraoperative ischemic-reperfusion injury in the cirrhotic liver.


METHODS
One percent dimethylnitrosamine (DMN) was administered intraperitoneally to liver cirrhosis (LC) groups of Wister rats on three consecutive days of each week for a period of four weeks. The rats were divided into a N60 group with 60 minute ischemia in normal livers, a LC60 group with 60 minute ischemia in cirrhotic livers, and a LC30 group with 30 minute ischemia in cirrhotic livers. Digital videotapes recorded with a pencil lens-probe charge-coupled device (CCD) microscope were analyzed with NIH Image software. In zone 3, the sinusoid diameter (SD) was measured and the volume fraction (Vv) of zone 3 was calculated in preischemia and after 10, 20, 30, and 60 min of reperfusion. At the same time, bile flow was measured.


RESULTS
The SD was significantly shorter in the cirrhotic liver groups than in the normal liver group at each point. The Vv after 60 min of reperfusion was significantly smaller in the LC60 group, with a survival rate of 0%, than in the LC30 group which had a survival rate of 67%. However, there was no significant difference in bile flow after 60 min of reperfusion in the LC30 and LC60 groups. Therefore, the Vv is suggested to be the better index for viability after ischemic-reperfusion.


CONCLUSIONS
SD and Vv indicate microcirculatory differences and indices in the normal and cirrhotic livers in preischemia during reperfusion.
Shimamoto
Tsuyoshi
T
First Department of Surgery, Gifu University School of Medicine, 40 Tsukasa-machi, Gifu City, Gifu 500-8705, Japan. t-shima@cc.gifu-u.ac.jp
Mori
Yoshio
Y
Takagi
Hisato
H
Yamada
Takuya
T
Sakamoto
Kenishi
K
Matsuo
Hiroshi
H
Nitta
Toyoo
T
Mizutani
Tomohiro
T
Iwata
Hisashi
H
Hirose
Hajime
H
D000818:Animals; D004128:Dimethylnitrosamine; D007511:Ischemia / Q000401:mortality; D008099:Liver / Q000098:blood supply; D008106:Liver Cirrhosis, Experimental / Q000139:chemically induced / Q000401:mortality / Q000473:pathology*; D008297:Male; D008833:Microcirculation; D008853:Microscopy; D051381:Rats; D017208:Rats, Wistar; D015996:Survival Rate; D013997:Time Factors
D004128:Dimethylnitrosamine