A potential suppressor of TGF-beta delays catagen progression in hair follicles.
TGF-beta plays important roles in the induction of catagen during the hair cycle. We examined whether TGF-beta2 could activate a caspase in human hair follicles. Using active caspase-9 and -3 specific antibodies, we found that TGF-beta2 activated these caspases in two regions, the lower part of the hair bulb and the outer layer of the outer root sheath. In addition, we searched for a plant extract that can effectively suppress TGF-beta action. We found that an extract of Hydrangea macrophylla reduced synthesis of a TGDbeta-inducible protein. We confirmed that the extract has a potential to promote hair elongation in the organ culture system. Furthermore, it delayed in vivo progression of catagen in a mouse model. Our results suggest that the induction of catagen by TGF-beta is mediated via activation of caspases and that a suppressor of TGF-beta could be effective in preventing male pattern baldness.
Tsuji
Yumiko
Y
Shiseido Life Science Research Center, Yokohama, Japan.
Denda
Sumiko
S
Soma
Tsutomu
T
Raftery
Laurel
L
Momoi
Takashi
T
Hibino
Toshihiko
T
D000818:Animals; D053148:Caspase 3; D053453:Caspase 9; D020169:Caspases / Q000378:metabolism; D046508:Culture Techniques; D004789:Enzyme Activation; D018859:Hair Follicle / Q000254:growth & development*; D006801:Humans; D031966:Hydrangea / Q000737:chemistry*; D051379:Mice; D008810:Mice, Inbred C57BL; D010936:Plant Extracts / Q000494:pharmacology*; D017395:Plasminogen Activator Inhibitor 1 / Q000096:biosynthesis; D016212:Transforming Growth Factor beta / Q000037:antagonists & inhibitors*; D053781:Transforming Growth Factor beta2
D010936:Plant Extracts; D017395:Plasminogen Activator Inhibitor 1; C509041:TGFB2 protein, human; D016212:Transforming Growth Factor beta; D053781:Transforming Growth Factor beta2; C505407:CASP3 protein, human; C506433:CASP9 protein, human; C505409:Casp3 protein, mouse; C506434:Casp9 protein, mouse; D053148:Caspase 3; D053453:Caspase 9; D020169:Caspases