[Molecular basis of preventive effect of human apolipoprotein-1 on murine vascular smooth muscle cell proliferation and lipid deposition induced by oxidized low density lipoprotein].
OBJECTIVE
To study the molecular basis of preventive effect of human apolipoprotein-1 (h-apoA-1) on vascular smooth muscle cell (vSMC) proliferation and lipid deposition induced by oxidized low density lipoprotein (ox-LDL).


METHODS
Smooth muscle cells originated from the aortae of h-apo-A-1 transgenic mice were cultured and divided into 2 groups, one group was stimulated by ox-LDL (tester) and the other group was used as control (driver). Subtractive hybridization was used to enrich the genes differentially expressed in the vSMCs induced by ox-LDL. A subtractive library was thus established and confirmed by colony hybridization in situ and dot blot analysis. 15 clones out of the 57 differentially expressed clones were randomly chosen foe sequencing and homology analysis. The whole-length cDNA library of vSMC induced by ox-LDL was established using SMART technique.


RESULTS
Three expression sequence tags (EST), all correlated with immune system, were confirmed: C1-inhibitor (C1-INH), lectin, and T cell receptor beta. The whole-length cDNA library contained 1.5 x 10(6) pfu/ml primary recombinants with insertions 0.5 - 3 kb in length.


CONCLUSIONS
The 3 EST may be involved in the mechanism of atherogenesis by ox-LDL and the mechanism of the function of h-ApoA-1 in retarding the progression of atherogenesis induced by ox-LDL.
Yu
Miao
M
Institute of Preclinical Medicine, Chinese Academy of Medical Sciences, Beijing 100005, China.
Gu
Su-min
SM
Zhou
Li
L
Zhao
Gao-feng
GF
Yu
Li-qing
LQ
Zhang
Hua
H
She
Ming-peng
MP
D000818:Animals; D016632:Apolipoprotein A-I / Q000235:genetics / Q000502:physiology*; D001161:Arteriosclerosis / Q000209:etiology*; D002455:Cell Division; D020224:Expressed Sequence Tags; D015870:Gene Expression; D006801:Humans; D050356:Lipid Metabolism; D008077:Lipoproteins, LDL / Q000633:toxicity*; D051379:Mice; D008822:Mice, Transgenic; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000378:metabolism
D016632:Apolipoprotein A-I; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein