Arginine selective biosensor based on arginase-urease immobilized in gelatin.
Arginase and urease enzymes were immobilized on the surface of pH electrode by using gelatin membrane which is then cross-linked with glutaraldehyde. Sensor response was maximum when 2.5 mM, Tris-HCl buffer (pH 8.5) was used at 25 degrees C. The biosensor response depends linearly on arginine concentration between 0.025-0.310 mM with response time 10 min. Furthermore, application of the system for the arginine detection in serum samples was also tested.
Karacaoğlu
Selma
S
Biochemistry Department, Faculty of Science, Ege University, Bornova-Izmir, Turkey.
Timur
Suna
S
Telefoncu
Azmi
A
D001119:Arginase / Q000378:metabolism*; D001120:Arginine / Q000097:blood*; D015374:Biosensing Techniques / Q000379:methods*; D002138:Calibration; D002624:Chemistry, Clinical / Q000379:methods; D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005780:Gelatin; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D014508:Urea / Q000097:blood; D014510:Urease / Q000378:metabolism*
D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D014508:Urea; D005780:Gelatin; D001120:Arginine; D014510:Urease; D001119:Arginase