Influence of copper(II) and magnesium(II) ions on the ciprofloxacin binding to DNA.
The influence of magnesium(II) and copper(II) ions on the binding of ciprofloxacin to double stranded calf thymus DNA was studied by fluorescence emission spectroscopy, ultraviolet- and circular dichroism (CD) spectroscopy. The interaction of ciprofloxacin and copper(II) ions was followed by strong fluorescence quenching which was almost unaffected by the presence of DNA. On the other hand, only a slight decrease in fluorescence emission intensity, which was enhanced in the presence of DNA, was observed for ciprofloxacin interaction with magnesium(II) ions. Furthermore, magnesium(II) ions increase the thermal stability of the DNA, while, in the presence of ciprofloxacin, the degree of stabilisation is smaller. In contrast, copper(II) ions destabilise double helical DNA to heat, while ciprofloxacin slightly affects only the second transition of the biphasic melting curve of calf thymus DNA. Magnesium(II) ions at 25 degrees C induce conformational transitions of DNA at concentrations of 1.5 mM and 2.5 M, as monitored by CD. On the other hand copper(II) ions induce only one conformational transition, at a concentration of 12.7 microM. At higher concentrations of copper(II) ions (c>700 microM) DNA starts to precipitate. Significant changes in the CD spectra of DNA were observed after addition of ciprofloxacin to a solution containing DNA and copper(II) ions, but not to DNA and magnesium(II) ions. Based on our spectroscopic results, we propose that copper(II) ions are not directly involved into ciprofloxacin binding to DNA via phosphate groups as it has been suggested for magnesium(II) ions.
Drevensek
Petra
P
Faculty of Chemistry and Chemical Technology, University of Ljubljana, Askerceva 5, POB 537, 1000, Ljubljana, Slovenia.
Turel
Iztok
I
Poklar Ulrih
Natasa
N
D000890:Anti-Infective Agents / Q000737:chemistry* / Q000378:metabolism; D002939:Ciprofloxacin / Q000737:chemistry* / Q000378:metabolism; D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D005453:Fluorescence; D008274:Magnesium / Q000737:chemistry*; D009691:Nucleic Acid Denaturation / Q000187:drug effects; D013057:Spectrum Analysis
D000890:Anti-Infective Agents; D002939:Ciprofloxacin; D003300:Copper; D004247:DNA; C427203:calf thymus DNA; D008274:Magnesium