title: Difference in anxiety and sensitization of the acoustic startle response between the two inbred mouse strains BALB/cAN and DBA/2N.
abstract: The inbred mouse strain BALB has been proposed to be an animal model for pathological anxiety. BALB exhibits a stronger acoustic startle response (ASR) than the 'less emotional' inbred strain DBA. Four experiments were conducted to determine whether this strong ASR is due to a higher anxiety level and/or to greater sensitization in BALB than in DBA, with the following results: (1) The ASR to the very first startle stimulus was found to be much stronger in BALB than in DBA, and freezing behavior evoked by startle stimuli was more pronounced in BALB than in DBA. These findings indicate a higher level of anxiety in this strain. (2) ASR amplitudes of BALB initially rose much higher during consecutive startle stimuli and remained at a high level much longer than in DBA. Thereafter, ASR amplitude dropped more slowly and to a lesser degree than in DBA. Startle amplitudes decreased similarly in both strains (strong exponential decrease) only when a low sound pressure level (SPL) was used which elicited approximately the same low ASR in both strains. These results can only be explained by increased sensitization in BALB. (3) The slope of the i/o-function, which represents the relation between sensory input and motor output, was steeper in BALB than in DBA. As it has been shown recently, sensitization increases the slope of the startle i/o-function indicating increased sensitization in BALB. It is discussed, however, whether anxiety also contributes to this effect. (4) Footshocks increased the ASR much less in BALB than in DBA, again showing increased sensitization in BALB. Both a higher level of anxiety and greater sensitization therefore determined the greater strength of the ASR in BALB than in DBA.
authors.affiliation: Universität Tübingen, Tierphysiologie Zoologisches Institut, Tübingen, Germany. claudia.plappert@uni-tuebingen.de
authors.forename: C F
authors.initials: CF
authors.lastname: Plappert
authors.forename: P K D
authors.initials: PK
authors.lastname: Pilz
mesh_terms: D000161:Acoustic Stimulation; D000818:Animals; D001143:Arousal / Q000235:genetics* / Q000502:physiology; D001921:Brain / Q000502:physiology*; D004597:Electroshock; D005260:Female; D006185:Habituation, Psychophysiologic / Q000235:genetics* / Q000502:physiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C / Q000235:genetics*; D008811:Mice, Inbred DBA / Q000235:genetics*; D010641:Phenotype; D013216:Reflex, Startle / Q000235:genetics* / Q000502:physiology; D013018:Sound Spectrography; D013045:Species Specificity