Myocardial contractility by strain echocardiography: comparison with physiological measurements in an in vitro model.
Strain echocardiography (SE) provides the rate and extent of myocardial segment shortening and lengthening. Thus we hypothesized that SE will noninvasively provide estimates of shortening velocity (SV) and length change (delta L). We compared SE-derived strain rate (SR) and strain (epsilon) to force/length transducer-derived SV and percent delta L in isolated muscle strips at multiple load levels and under varying conditions. Electrically stimulated contractions in left ventricular muscle strips (n = 20) were simultaneously recorded with a force/length transducer (to measure SV and percent delta L) and an ultrasound transducer (to measure SR and epsilon). Measurements were made at baseline, after inotropic stimulation, and during hypoxia at multiple load clamp levels (simulating multiple levels of afterload). Despite a difference in absolute numbers, there was a close correlation between SR and SV at baseline (R2 = 0.95), with dobutamine treatment (R2 = 0.99), and during hypoxia (R2 = 0.99). SR was load dependent at baseline (r = 0.98), with dobutamine treatment (r = 0.99), and during hypoxia (r = 0.92). Similarly, there was a close correlation between epsilon and delta L at baseline (R2 = 0.99), with dobutamine treatment (R2 = 0.96), and during hypoxia (R2 = 0.87). Percent epsilon was load dependent at baseline (r = 0.98), with dobutamine treatment (r = 0.98), and during hypoxia (r = 0.94). Bland-Altman analysis revealed a systematic overestimation of SV by SE-derived SR at baseline and with dobutamine treatment. There was no bias with SR measurements during hypoxia or with epsilon measurements. SE closely tracks standard physiological parameters of regional contractile function, such as SV and delta L, under conditions of varying afterload.
Abraham
Theodore P
TP
Division of Cardiovascular Diseases, Mayo Clinic and Foundation, Rochester, Minnesota 55905, USA. Abraham.Theodore@mayo.edu
Laskowski
Carl
C
Zhan
Wen-Zhi
WZ
Belohlavek
Marek
M
Martin
Elizabeth A
EA
Greenleaf
James F
JF
Sieck
Gary C
GC
D000818:Animals; D004452:Echocardiography; D004558:Electric Stimulation; D000860:Hypoxia / Q000503:physiopathology; D066298:In Vitro Techniques; D008297:Male; D009200:Myocardial Contraction / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016474:Weight-Bearing / Q000502:physiology