[Structural mechanism studies of hTNF alpha mutants in position 30 and 42 amino acid].
OBJECTIVE
To study the relationship between the structure and functional activity of hTNF alpha.


METHODS
Four hTNF alpha mutants were constructed, different binding structures and gene responses related with these mutants were studied by the methods of immunoprecipitation and mRNA differential display.


RESULTS
The specific activities and LD50 of the different hTNF alpha mutants indicated their different bioactivities. It was shown that the hTNF alpha mutants had the relative binding affinities to the wild types. The mRNA differential display assay proved that the hTNF alpha mutants stimulated different gene responses.


CONCLUSIONS
These results suggest that the specific anti-tumor activities of hTNF alpha mutants are accomplished by inducing different or same gene response at different quantities after its binding to specific receptor.
Hu
Fang
F
Department of Immunology, Institute of Medical Biology, CAMS, PUMC, Kunming 650118, China.
Dong
Shao-zhong
SZ
Liu
Long-ding
LD
He
Shao-qing
SQ
Zhao
Shu-dong
SD
Li
Qi-han
QH
D020816:Amino Acid Motifs; D017209:Apoptosis; D001665:Binding Sites; D020869:Gene Expression Profiling; D006801:Humans; D015394:Molecular Structure; D009154:Mutation; D013329:Structure-Activity Relationship; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000502:physiology
D014409:Tumor Necrosis Factor-alpha