Comparative study of fibronectin gene expression in tissues from hypertrophic scars and diabetic foot ulcers.
OBJECTIVE
To explore the expression characteristic of fibronectin gene in hypertrophic scars and diabetic ulcer tissues.


METHODS
The biopsies from normal skins, hypertrophic scars and diabetic foot ulcers were taken. The technique of quantitative polymerase chain reaction (PCR) was used to evaluate the gene expression of fibronectin in the above biopsies.


RESULTS
Fibronectin gene expression was enhanced in hypertophic scars and decreased in diabetic foot ulcers compared with that in normal skins. Quantitative comparison showed about 2-fold increase of fibronectin mRNA level in hypertrophic scars and about 3-fold decrease of fibronectin mRNA level in diabetic ulcers as compared with that in normal skins.


CONCLUSIONS
Fibronectin gene expression is influenced by the tissue environment. Different expression and synthesis of fibronectin may cause different outcomes in wound healing.
Fu
Xiaobing
X
Wound Healing Unit, Burn Institute, 304th Hospital, Beijing 100037.
Yang
Yinhui
Y
Sun
Tongzhu
T
Wang
Yaping
Y
Sheng
Zhiyong
Z
D000293:Adolescent; D000328:Adult; D000368:Aged; D017439:Cicatrix, Hypertrophic / Q000378:metabolism*; D017719:Diabetic Foot / Q000378:metabolism*; D005260:Female; D005353:Fibronectins / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics
D005353:Fibronectins; D012333:RNA, Messenger