High TNT-transforming activity by a mixed culture acclimated and maintained on crude-oil-containing media.
A mixed microbial culture originating from a petroleum-contaminated site and maintained on crude oil exhibited high 2,4,6-trinitrotoluene (TNT) transformation activity. Cultivation of the mixed culture in glucose-containing medium for 29 h resulted in almost complete transformation of 100 ppm TNT. TNT transformation was observed with both growing and resting cells. With subculturing, it was found that TNT could support growth of the mixed culture when supplied as sole carbon source, sole nitrogen source, or sole carbon and nitrogen source. The finding that a mixed microbial culture maintained on crude oil exhibited high TNT transformation activity without prior subculture on TNT-containing media is novel and may have potential practical applications in the bioremediation of munitions-contaminated soil and wastewater.
Popesku
Jason T
JT
Department of Biology, University of Waterloo, Waterloo, ON N2L 3G1, Canada.
Singh
Ajay
A
Zhao
Jian-Shen
JS
Hawari
Jalal
J
Ward
Owen P
OP
D001673:Biodegradation, Environmental; D001711:Biotransformation; D002244:Carbon / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D010578:Petroleum / Q000378:metabolism / Q000382:microbiology; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D014303:Trinitrotoluene / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism
D010578:Petroleum; D012989:Soil Pollutants; D014874:Water Pollutants, Chemical; D014303:Trinitrotoluene; D002244:Carbon; D005947:Glucose; D009584:Nitrogen