Accelerated alveolar bone loss in mice lacking interleukin-10.
Interleukin-10 regulates pro-inflammatory cytokines, including those implicated in alveolar bone resorption. We hypothesized that lack of interleukin-10 leads to increased alveolar bone resorption. Male interleukin-10(-/-) mice, on 129/SvEv and C57BL/6J background, were compared with age-, sex-, and strain-matched interleukin-10(+/+) controls for alveolar bone loss. Immunoblotting was used for analysis of serum reactivity against bacteria associated with colitis and periodontitis. Interleukin-10(-/-) mice had significantly greater alveolar bone loss than interleukin-10(+/+) mice (p = 0.006). The 30-40% greater alveolar bone loss in interleukin-10(-/-) mice was evident in both strains, with C57BL/6J interleukin-10(-/-) mice exhibiting the most bone loss. Immunoblotting revealed distinct interleukin-10(-/-) serum reactivity against Bacteroides vulgatus, B. fragilis, Prevotella intermedia, and, to a lesser extent, against B. forsythus. The results of the present study suggest that lack of interleukin-10 leads to accelerated alveolar bone loss.
Al-Rasheed
A
A
Department of Periodontics, School of Dentistry, Loma Linda University, Loma Linda, CA, USA.
Scheerens
H
H
Rennick
D M
DM
Fletcher
H M
HM
Tatakis
D N
DN
D016301:Alveolar Bone Loss / Q000145:classification / Q000276:immunology* / Q000382:microbiology; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001439:Bacteroides / Q000276:immunology; D001441:Bacteroides fragilis / Q000276:immunology; D015151:Immunoblotting; D016753:Interleukin-10 / Q000097:blood / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D016966:Porphyromonas gingivalis / Q000276:immunology; D018713:Prevotella intermedia / Q000276:immunology; D018709:Statistics, Nonparametric
D000907:Antibodies, Bacterial; D016753:Interleukin-10