[Genotyping of HLA-DRB1 by PCR-SSP in Yunnan Lahu ethnic groups].
OBJECTIVE
To investigate polymorphism of HLA-DRB1 in Chinese Lahu population in Yunnan.


METHODS
Polymerase chain reaction-sequence specific primers (PCR-SSP) were used to determine HLA-DRB1 genotypes of 110 unrelated healthy Lahu minority people of Yunnan Province.


RESULTS
Sixteen alleles of DRB1 were detected in this study. The results of test showed that the genotype distributions observed were corresponded with the Hardy-Weinberg equilibrium.


CONCLUSIONS
This study has obtained a more comprehensive and accurate data set of the normal allele frequencies of HLA-DRB1 in Chinese Lahu population in Yunnan Province, which may be of significance in the studies on population genetics and disease association.
Hong
Kun-xue
KX
Department of Genetics, Institute of Medical Biology, CAMS, PUMC, Kunming 650118, China.
He
Qin
Q
San
Xi-yun
XY
Yu
Jian-kun
JK
Lin
Ke-qin
KQ
Shi
Lei
L
Chu
Jia-you
JY
D044466:Asian People; D017931:DNA Primers; D005006:Ethnicity; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods
D017931:DNA Primers; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains