title: Pair-dependent co-aggregation behavior of non-flocculating sludge bacteria.
abstract: Two strains of non-flocculating sewage sludge bacteria (Xanthomonas sp. S53 and Microbacterium esteraromaticum S51) showed 91% and 77% co-aggregation, respectively, with Acinetobacter johnsonii S35 using a spectrophometric assay. The co-aggregates in case of Xanthomonas sp. S53 and A. johnsonii S35 were above 100 microm and stable against EDTA (2 mM) and a commercial protease (0.2 mg ml(-1)). Protease/periodate pretreatment of the partners did not affect this co-aggregation. On the other hand, co-aggregates of M. esteraromaticum S51 and A. johnsonii S35 (50-70 microm) were deflocculated by EDTA or protease. Protease pretreatment of M. esteraromaticum S51 and periodate pretreatment of A. johnsonii S35 prevented their co-aggregation with respective untreated partners. The potential co-aggregation mechanisms of A. johnsonii S35 varied depending upon the other partner involved.
authors.affiliation: Department of Applied Chemistry, Faculty of Engineering, Utsunomiya University, 7-1-2 Yoto, Utsunomiya 321-8585, Japan.
authors.forename: Anushree
authors.initials: A
authors.lastname: Malik
authors.forename: Kazuo
authors.initials: K
authors.lastname: Kakii
mesh_terms: D000150:Acinetobacter / Q000166:cytology / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D001422:Bacterial Adhesion / Q000187:drug effects / Q000502:physiology*; D018441:Biofilms; D004492:Edetic Acid / Q000494:pharmacology; D005427:Flocculation; D057927:Hydrophobic and Hydrophilic Interactions; D008835:Micrococcaceae / Q000166:cytology / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D010504:Periodic Acid / Q000494:pharmacology; D012697:Serine Endopeptidases / Q000494:pharmacology; D012722:Sewage / Q000382:microbiology*; D014974:Xanthomonas / Q000166:cytology / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D012722:Sewage; D010504:Periodic Acid; D004492:Edetic Acid; C009288:metaperiodate; D012697:Serine Endopeptidases; C028734:protease E