The cytochrome c3-[Fe]-hydrogenase electron-transfer complex: structural model by NMR restrained docking.
Cytochrome c(3) (M(r) 13000) is a low redox potential cytochrome specific of the anaerobic metabolism in sulfate-reducing bacteria. This tetrahemic cytochrome is an intermediate between the [Fe]-hydrogenase and the cytochrome Hmc in Desulfovibrio vulgaris Hildenborough strain. The present work describes the structural model of the cytochrome c(3)-[Fe]-hydrogenase complex obtained by nuclear magnetic resonance restrained docking. This model connects the distal cluster of the [Fe]-hydrogenase to heme 4 of the cytochrome, the same heme found in the interaction with cytochrome Hmc. This result gives evidence that cytochrome c(3) is an electron shuttle between the periplasmic hydrogenase and the Hmc membrane-bound complex.
ElAntak
Latifa
L
Unité de Bioénergétique et Ingénierie des Protéines, IBSM-CNRS, 31 chemin Joseph Aiguier, 13402 Cedex 20, Marseille, France. guerlesq@ibsm.cnrs-mrs.fr
Morelli
Xavier
X
Bornet
Olivier
O
Hatchikian
Claude
C
Czjzek
Mirjam
M
Dolla
Alain
A
Guerlesquin
Françoise
F
D003574:Cytochrome c Group / Q000737:chemistry / Q000378:metabolism; D016969:Desulfovibrio vulgaris / Q000737:chemistry; D004579:Electron Transport; D008565:Membrane Proteins; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000737:chemistry* / Q000378:metabolism; D033903:Periplasmic Proteins; D011485:Protein Binding
D003574:Cytochrome c Group; D008565:Membrane Proteins; D033903:Periplasmic Proteins; C017165:cytochrome c(3); D010088:Oxidoreductases; C019981:cytochrome c3 hydrogenase