title: A Burkholderia pseudomallei type III secreted protein, BopE, facilitates bacterial invasion of epithelial cells and exhibits guanine nucleotide exchange factor activity.
abstract: We report the characterization of BopE, a type III secreted protein that is encoded adjacent to the Burkholderia pseudomallei bsa locus and is homologous to Salmonella enterica SopE/SopE2. Inactivation of bopE impaired bacterial entry into HeLa cells, indicating that BopE facilitates invasion. Consistent with this notion, BopE expressed in eukaryotic cells induced rearrangements in the subcortical actin cytoskeleton, and purified BopE exhibited guanine nucleotide exchange factor activity for Cdc42 and Rac1 in vitro.
authors.affiliation: Division of Environmental Microbiology, Institute for Animal Health, Compton Laboratory, Berkshire RG20 7NN, United Kingdom.
authors.forename: Mark P
authors.initials: MP
authors.lastname: Stevens
authors.forename: Andrea
authors.initials: A
authors.lastname: Friebel
authors.forename: Lowrie A
authors.initials: LA
authors.lastname: Taylor
authors.forename: Michael W
authors.initials: MW
authors.lastname: Wood
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Brown
authors.forename: Wolf-Dietrich
authors.initials: WD
authors.lastname: Hardt
authors.forename: Edouard E
authors.initials: EE
authors.lastname: Galyov
mesh_terms: D000199:Actins / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D016957:Burkholderia pseudomallei / Q000472:pathogenicity*; D020662:Guanine Nucleotide Exchange Factors / Q000378:metabolism*; D006367:HeLa Cells / Q000382:microbiology; D006801:Humans; D014774:Virulence; D020764:cdc42 GTP-Binding Protein / Q000378:metabolism*; D020830:rac1 GTP-Binding Protein / Q000378:metabolism*
chemical_list: D000199:Actins; D001426:Bacterial Proteins; D020662:Guanine Nucleotide Exchange Factors; C103987:SopE protein, Salmonella; D020764:cdc42 GTP-Binding Protein; D020830:rac1 GTP-Binding Protein