[Experimental study of repairing segmental bone defect with reconstituted freeze-dried bone allograft].
OBJECTIVE
To study the effect of basic fibroblast growth factor (bFGF) and hyaluronic acid gel (HAG) combined with freeze-dried bone allograft in repairing segmental bone defect and to explore their mechanism.


METHODS
The 15 mm segmental bone/periosteum defects were created on bilateral radius in 50 New Zealand rabbits and were treated with four different kinds of implants on 25 radius respectively (group A: bFGF and HAG combined with freeze-dried bone; group B: bFGF combined with freeze-dried bone; group C: HAG combined with freeze-dried bone; group D: simple freeze-dried bone as a control). The repair of defect was observed radiologically and histologically and were analyzed by radionuclide bone imaging and measurement of calcium contents at different periods.


RESULTS
The new bone formation, bone metabolic activity and calcium contents of defects were higher in group A than in group B (P < 0.05), and were higher in group B than in groups C and D (P < 0.05). There were no significant difference between groups C and D. The bone defects healed in the 8th week in group A, in the 10th week in group B, but did not healed in the 10th week in groups C and D.


CONCLUSIONS
As an osteogenetic factor, bFGF promotes the new bone formation; as a slow-release carrier, HAG enhances the effectiveness of bFGF. The combination of bFGF, HAG and freeze-dried bone allograft can repair the segmental bone defect more effectively.
Chen
Qing
Q
Department of Orthopedic Surgery, Central Hospital of Wuhan, Wuhan, Hubei, P. R. China 430014.
Gu
Jie-fu
JF
Cai
Lin
L
D000818:Animals; D001861:Bone Regeneration; D016025:Bone Transplantation / Q000379:methods*; D002118:Calcium / Q000032:analysis; D016222:Fibroblast Growth Factor 2 / Q000627:therapeutic use*; D017102:Fracture Healing; D005612:Freeze Drying; D005782:Gels / Q000627:therapeutic use*; D007085:Ilium / Q000637:transplantation; D011817:Rabbits; D011859:Radiography; D011884:Radius / Q000000981:diagnostic imaging / Q000293:injuries / Q000601:surgery*; D019651:Plastic Surgery Procedures; D014184:Transplantation, Homologous
D005782:Gels; D016222:Fibroblast Growth Factor 2; D002118:Calcium