A SNARE required for retrograde transport to the endoplasmic reticulum.
SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) are central components of the machinery mediating membrane fusion in all eukaryotic cells. Sequence analysis of the yeast genome revealed a previously uncharacterized SNARE, SNARE-like tail-anchored protein 1 (Slt1). Slt1 is an essential protein localized in the endoplasmic reticulum (ER). It forms a SNARE complex with Sec22 and the ER syntaxin Ufe1. Down-regulation of Slt1 levels leads to improper secretion of proteins normally resident in the ER. We suggest that Slt1 is a component of the SNAREpin required for retrograde traffic to the ER. Based on the previously reported association with Ufe1 and Sec22, Sec20 likely contributes the fourth SNARE to the SNAREpin.
Burri
Lena
L
Russell Grimwade School of Biochemistry and Molecular Biology, University of Melbourne, Parkville 3010, Australia.
Varlamov
Oleg
O
Doege
Claudia A
CA
Hofmann
Kay
K
Beilharz
Traude
T
Rothman
James E
JE
Söllner
Thomas H
TH
Lithgow
Trevor
T
D000595:Amino Acid Sequence; D001693:Biological Transport, Active; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D016681:Genome, Fungal; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D050767:Qc-SNARE Proteins; D050600:SNARE Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D033921:Vesicular Transport Proteins
D008565:Membrane Proteins; D050767:Qc-SNARE Proteins; D050600:SNARE Proteins; D029701:Saccharomyces cerevisiae Proteins; C477343:Use1 protein, S cerevisiae; D033921:Vesicular Transport Proteins