[Study on the function of HSD-3.8 gene encoding a testis-specific protein with yeast two-hybrid system].
OBJECTIVE
To explore the protein factors that could interact with the testis-specific protein encoded by HSD-3.8 gene (GenBank Accession Number AF311312) related with female fertilization.


METHODS
Yeast two-hybrid system was used to screen the human ovary MATCHMAKER cDNA library with constructed "bait plasmid" containing the 0.7 kb fragment (HSD-0.7) of HSD-3.8. The interaction with the positive fragments using a series of truncated bait plasmids was investigated.


RESULTS
One positive gene fragment was obtained, which coded for 144 amino acids of the C-terminus of human G protein beta subunit 1. Truncated bait plasmids couldn't interact with the fish protein fragment in yeast.


CONCLUSIONS
The protein encoded by HSD-3.8 gene may function through G protein signal transduction pathway and the interaction depends on the integration of the bait protein.
Lin
Wen
W
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China.
Miao
Shi-ying
SY
Zhang
Lin
L
Wang
Lin-fang
LF
D000255:Adenosine Triphosphate / Q000378:metabolism; D000328:Adult; D000954:Antigens, Surface; D004268:DNA-Binding Proteins / Q000235:genetics; D005260:Female; D019204:GTP-Binding Proteins / Q000235:genetics* / Q000502:physiology; D015723:Gene Library; D006801:Humans; D008297:Male; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D013094:Spermatozoa / Q000737:chemistry / Q000502:physiology; D016708:Synaptophysin; D013737:Testis / Q000737:chemistry*; D020798:Two-Hybrid System Techniques; D015003:Yeasts / Q000235:genetics
D000954:Antigens, Surface; D004268:DNA-Binding Proteins; D011506:Proteins; D016708:Synaptophysin; D000255:Adenosine Triphosphate; D019204:GTP-Binding Proteins; C483942:SPAG1 protein, human