title: [Induced expression of dopamine D2 receptor in human neuro-progenitor cell line HNG1210-E].
abstract: OBJECTIVE
  To investigate whether the dopamine receptor D2 can express or can be induced to express in HNG1210-E, a monoclonal, telomerase-immortalized, human neural progenitor cell line.
  
  
  METHODS
  By means of RT-PCR, immuno-fluorescent staining, and fluo3 Ca2+ imaging to the expression of D2 mRNA and protein as well as the reaction to dopamine were demonstrated.
  
  
  RESULTS
  HNG1210-E cells could be induced to express D2 mRNA and its proteins. The induced cells also reacted to dopamine (5 mmol.L-1), which caused rapid rising of cytoplasm Ca2+.
  
  
  CONCLUSIONS
  HNG1210-E cells can be induced to express D2 mRNA and functional proteins.
authors.affiliation: Peking University Stem Cell Research Center, Beijing 100083, China.
authors.forename: Qikuan
authors.initials: Q
authors.lastname: Hu
authors.forename: Changsheng
authors.initials: C
authors.lastname: Lin
authors.forename: Yun
authors.initials: Y
authors.lastname: Bai
authors.forename: Jie
authors.initials: J
authors.lastname: Bai
authors.forename: Qihua
authors.initials: Q
authors.lastname: He
authors.forename: Li
authors.initials: L
authors.lastname: Shen
authors.forename: Lingsong
authors.initials: L
authors.lastname: Li
mesh_terms: D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D003593:Cytoplasm / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D006801:Humans; D009474:Neurons / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D017448:Receptors, Dopamine D2 / Q000032:analysis / Q000235:genetics*; D013234:Stem Cells / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D017448:Receptors, Dopamine D2; D002118:Calcium