Sensitive determination of tenofovir in human plasma samples using reversed-phase liquid chromatography.
A new high-performance liquid chromatography assay was developed for the determination of tenofovir, a nucleotide analogue, in plasma. A solid-liquid extraction procedure was coupled with a reversed-phase HPLC system. The system requires a mobile phase containing Na(2)HPO(4) buffer, tetrabutylammonium hydrogen sulfate and acetonitrile for different elution through a C(18) column with UV detection. The method proved to be accurate, precise and linear between 10 and 4000 ng/ml. The method was applied to determine trough levels of tenofovir in 11 HIV-infected patients with virologic failure under multiple antiretroviral therapy. This method was also successfully applied to a pharmacokinetic study in an HIV infected patient with renal failure.
Sentenac
S
S
Clinical Pharmacology and Drug Monitoring Unit, Pitie-Salpetriere Hospital, Paris, France.
Fernandez
C
C
Thuillier
A
A
Lechat
P
P
Aymard
G
G
D000225:Adenine / Q000031:analogs & derivatives* / Q000097:blood* / Q000493:pharmacokinetics / Q000627:therapeutic use; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016903:Drug Monitoring; D015658:HIV Infections / Q000097:blood* / Q000188:drug therapy; D006801:Humans; D063065:Organophosphonates; D009943:Organophosphorus Compounds / Q000097:blood* / Q000493:pharmacokinetics / Q000627:therapeutic use; D015203:Reproducibility of Results; D018894:Reverse Transcriptase Inhibitors / Q000097:blood* / Q000493:pharmacokinetics / Q000627:therapeutic use; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D000068698:Tenofovir
D063065:Organophosphonates; D009943:Organophosphorus Compounds; D018894:Reverse Transcriptase Inhibitors; D000068698:Tenofovir; D000225:Adenine