title: Role of microfilaments and microtubules in the invasion of INT-407 cells by Campylobacter jejuni.
abstract: The internalization mechanisms triggered by Campylobacter jejuni were studied by invasion assays conducted with different inhibitors that act on the cytoskeleton structure of eukaryotic cells. The depolymerization of microfilaments by cytochalasin-D and that of microtubules by colchicines and nocodazole inhibited the uptake of C. jejuni into INT-407 cells in a dose-dependent manner. The inhibitory effect of microfilament depolymerization on C. jejuni internalization was more pronounced than that of microtubule depolymerization. By immunofluorescence microscopic observations, it was demonstrated that both microfilaments and microtubules were localized in INT-407 cells after C. jejuni infection. These data suggest that the internalization mechanism triggered by C. jejuni is associated with the combined effect of microfilaments and microtubules of host cells.
authors.affiliation: Laboratory of Veterinary Public Health, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Debabrata
authors.initials: D
authors.lastname: Biswas
authors.forename: Kikuji
authors.initials: K
authors.lastname: Itoh
authors.forename: Chihiro
authors.initials: C
authors.lastname: Sasakawa
mesh_terms: D008841:Actin Cytoskeleton / Q000502:physiology*; D000199:Actins / Q000378:metabolism; D001704:Biopolymers; D016123:Campylobacter jejuni / Q000472:pathogenicity / Q000502:physiology*; D002478:Cells, Cultured / Q000187:drug effects / Q000648:ultrastructure; D003078:Colchicine / Q000494:pharmacology; D015638:Cytochalasin D / Q000494:pharmacology; D003599:Cytoskeleton / Q000648:ultrastructure; D004305:Dose-Response Relationship, Drug; D004705:Endocytosis / Q000187:drug effects / Q000502:physiology*; D004847:Epithelial Cells / Q000187:drug effects / Q000382:microbiology / Q000648:ultrastructure; D006801:Humans; D007422:Intestines / Q000166:cytology / Q000196:embryology / Q000382:microbiology* / Q000648:ultrastructure; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000187:drug effects / Q000502:physiology*; D015739:Nocodazole / Q000494:pharmacology; D013045:Species Specificity; D014404:Tubulin / Q000378:metabolism; D014774:Virulence / Q000502:physiology
chemical_list: D000199:Actins; D001704:Biopolymers; D014404:Tubulin; D015638:Cytochalasin D; D015739:Nocodazole; D003078:Colchicine