[Somatostatin receptor subtype 2 mRNA expression in human gastric immortalized cell line and tumor cell lines].
OBJECTIVE
Inhibition of somatostatin on cell proliferation via somatostatin receptor subtype 2 (SS-R2), suggested this receptor subtype may be involved in tumor growth. Present study is to demonstrate whether SS-R2 was expressed in tumor cell lines extensively as well as in human gastric epithelia immortalized cell line. Meanwhile, the effect of p53 mutation on SS-R2 expression in gastric cancer cell line was also elucidated.


METHODS
Full-length human somatostatin receptor subtype 2 cDNA was cloned by RT-PCR. Using cDNA as the probe and RT-PCR, expression of mRNA for SS-R2 in 9 cancer cell lines and human gastric immortalized cell line was detected.


RESULTS
Data revealed that most cancer cell lines but a pancreatic cancer cell line expressed SS-R2 mRNA.


CONCLUSIONS
Regardless whether p53 mutation or not in human gastric cancer cell lines, SS-R2 mRNA was transcribed.
Chen
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Y
Department of Gastroenterology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
Chen
Y
Y
D002460:Cell Line; D045744:Cell Line, Tumor; D005753:Gastric Mucosa / Q000166:cytology; D015972:Gene Expression Regulation, Neoplastic; D016158:Genes, p53 / Q000235:genetics; D006801:Humans; D009154:Mutation; D012333:RNA, Messenger / Q000096:biosynthesis; D017481:Receptors, Somatostatin / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger; D017481:Receptors, Somatostatin; C093733:somatostatin receptor 2