[The changes and significance of red cell nature-immune-adhesion function in liver diseases at different stages].
OBJECTIVE
To study the changes of red cells nature immune adhesion function (RNIAF) in liver diseases at different stages, and the feasibility of RNIAF in evaluating the severity of liver diseases.


METHODS
Venous blood was extracted from 682 patients with liver disease, including cirrhosis, chronic hepatitis, acute hepatitis, hepatitis gravis, and 50 healthy blood donors as controls. Suspension of red cells in self-plasma was mixed with solution of mouse ascites carcinoma cells. A tumor cell attached with 5 red cells or 2 lymphocytes/granulocytes was counted as one rosette. The adhesion rate was calculated. Serum soluble complement receptor 1 (sCR1) was measured with a newly established sandwich enzyme-linked immunosorbent assay (ELISA). The CR1 expressed on erythrocytes has assayed by cell-ELISA.


RESULTS
The expression of CR1 on erythrocytes was lower among the patients with liver diseases. Compared with that in healthy controls, RNIAF decreased among the patients with various liver diseases (all P < 0.01) in the order of acute hepatitis, chronic hepatitis, cirrhosis Child's A, cirrhosis Child's B, cirrhosis Child's C, and hepatitis gravis. CHE and PTA decreased and PT increased among the patients with cirrhosis and hepatitis gravis (all P < 0.05 or P < 0.01). RNIAF was positively correlated with CHE, PT, and PTA in patents with cirrhosis. CHE, PT and PTA remained at low levels while RNIAF almost returned to its normal level in the convalescents of hepatitis gravis. CR1 expressed on erythrocytes and sCR1 in serum in various liver diseases were decreased at different degrees, however, they changed significantly later than RNIAF. Serum sCR1 significantly increased in the patients with cirrhosis, particularly in those in grades Child's A to C.


CONCLUSIONS
Low-cost, easy to operate and with early change and stable results, RNIAF may be used as an important indicator to evaluate the severity of liver diseases. of CHE, PT/PTA. It is an important to evaluate the severity of liver function in clinic.
Wang
Hai-bin
HB
Center for Clinic Laboratory Medicine, 302nd Hospital of the People's Liberation Army, Beijing 100039, China.
Zhang
Ji-wan
JW
Guo
Jing-xia
JX
Liu
Zhen-hong
ZH
D002448:Cell Adhesion; D002802:Cholinesterases / Q000097:blood; D004912:Erythrocytes / Q000276:immunology / Q000502:physiology*; D006801:Humans; D008099:Liver / Q000503:physiopathology; D008107:Liver Diseases / Q000097:blood* / Q000503:physiopathology; D017463:Receptors, Complement 3b / Q000097:blood
D017463:Receptors, Complement 3b; D002802:Cholinesterases