[Screening the trans-action factors binding to the enhancer element of rat GST-P by yeast one-hybrid system].
OBJECTIVE
To investigate the diversity of the mechanisms of rat glutathione S-transferase P (GST-P) gene expression regulation and the relation between rat GST-P gene expression and carcinogenesis. To search the trans-action factors binding to the enhancer element of rat GST-P (glutathione S-transferase P enhancer, GPE I).


METHODS
We searched the trans-action factors binding to GPE I using yeast one-hybrid system, and analyzed the DNA sequence by sequencing and computer search.


RESULTS
Two positive clones pYGPE1 and pYGPE2 were obtained. The homologies of the insert sequence of pYGPE1 nucleotide and amino acid sequences with rat c-jun cDNA were 99% and 100% respectively; the homologies of the insert sequence of pYGPE2 nucleotide and amino acid sequences with rat mitochondrial adenine nucleotide translocase cDNA were 99% and 100% respectively.


CONCLUSIONS
Rat c-Jun and mitochondrial adenine nucleotide translocase can bind to the GPE I core sequence, and they perhaps are the trans-action factors of GPE I.
Liao
M
M
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China.
Zuo
J
J
Liu
D
D
Fang
F
F
D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D004742:Enhancer Elements, Genetic / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D016758:Genes, jun / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics*; D033704:Nucleotide Transport Proteins / Q000235:genetics; D051381:Rats; D017421:Sequence Analysis; D017385:Sequence Homology; D015534:Trans-Activators / Q000235:genetics*; D015003:Yeasts / Q000235:genetics
D018076:DNA, Complementary; D033704:Nucleotide Transport Proteins; D015534:Trans-Activators; D005982:Glutathione Transferase