Synthesis and properties of 5'-amino-2',4'-BNA modified oligonucleotides with P3'-->N5' phosphoramidate linkages.
5'-Amino-2',4'-BNA, a novel analogue of BNA series compounds, was synthesized and its incorporation into oligonucleosides via P3'-->N5' phosphoramidate linkage was successfully achieved on a DNA automatic synthesizer. The 5'-amino-2',4'-BNA modified oligonucleotides were found to have a potent hybridizing affinity for the single stranded RNA and double stranded DNA complements, and also to show a good resistant ability against SVPDE.
Obika
Satoshi
S
Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
Nakagawa
Osamu
O
Hiroto
Akiko
A
Hari
Yoshiyuki
Y
Imanishi
Takeshi
T
D000577:Amides / Q000737:chemistry*; D001483:Base Sequence; D009696:Nucleic Acids / Q000138:chemical synthesis* / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry; D010756:Phosphoric Acids / Q000737:chemistry*
D000577:Amides; D009696:Nucleic Acids; D009841:Oligonucleotides; D010756:Phosphoric Acids; C011067:phosphoramidic acid