A novel fluorescent nonnatural amino acid that can be incorporated into a specific position of streptavidin.
A novel highly fluorescent nonnatural amino acid (beta-(N-methylanthraniloyl)-L-alpha,beta-diaminopropionic acid; nmaDap) was incorporated at the 120th position of streptavidin. The position was directed by a CGGG/CCCG four-base codon/anticodon pair. The incorporation efficiency and the biotin-binding ability of the nmaDap mutant were compared to those of beta-anthraniloyl-L-alpha,beta-diaminopropionic acid (atnDap).
Taki
Masumi
M
Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, 3-1-1 Tsushimanaka, Okayama 700-8530, Japan.
Hohsaka
Takahiro
T
Murakami
Hiroshi
H
Kuno
Atsushi
A
Hasegawa
Tsunemi
T
Taira
Kazunari
K
Sisido
Masahiko
M
D000596:Amino Acids / Q000737:chemistry*; D015153:Blotting, Western; D005456:Fluorescent Dyes / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry*
D000596:Amino Acids; D005456:Fluorescent Dyes; D019809:Streptavidin