Ventral nerve cord remodeling in a stingless bee (Melipona quadrifasciata anthidioides, Hymenoptera, Apidae) depends on ecdysteroid fluctuation and programmed cell death.
The reorganization of the ventral nerve cord (VNC) during metamorphosis of M. quadrifasciata was observed to be characterized by shortening of connectives and subsequent fusion of the 2nd and 3rd thoracic and the 1st abdominal ganglia. Also, the 5th to 7th abdominal ganglia came into very close contact. These changes were accompanied by increasing levels of endogenous ecdysteroids, as determined by a radioimmunoassay. Incubation of VNC in the presence of 5 microg 20-hydroxyecdysone, caused significant shortening of connectives in the thoracic region, but not in the abdomen, evidencing a segment-specific response to this hormone. Cell death in the ventral ganglia was revealed by transmission electron microscopy and TUNEL-reaction. Detection of labeled cells in the region where contiguous ganglia come into close contact suggests that programmed cell death is involved in ganglionic fusion.
Pinto
Lucimara Z
LZ
Departamento de Genética, FMRP, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
Laure
Marcela A F B
MA
Bitondi
Márcia M G
MM
Hartfelder
Klaus
K
Simões
Zilá L P
ZL
D000818:Animals; D017209:Apoptosis; D001516:Bees / Q000502:physiology*; D026461:Ecdysteroids / Q000378:metabolism*; D020287:In Situ Nick-End Labeling; D008675:Metamorphosis, Biological; D008854:Microscopy, Electron; D009417:Nerve Tissue / Q000502:physiology* / Q000648:ultrastructure*; D017933:Peripheral Nervous System / Q000502:physiology* / Q000648:ultrastructure*; D011863:Radioimmunoassay; D013997:Time Factors
D026461:Ecdysteroids