title: Anti-obesity effect of Dioscorea nipponica Makino with lipase-inhibitory activity in rodents.
abstract: In the process of screening for pancreatic lipase inhibitors, which could be used as an anti-obesity measure, the methanol extract of Dioscorea nipponica Makino powder (DP) appeared to have potent inhibitory activity against porcine pancreatic lipase with an IC50 value of 5-10 microg/ml, where the enzyme activity was assayed by using 4-methylumbelliferyl oleate as a substrate. Further purification of active components present in the herb generated dioscin that belongs to the saponin family. Dioscin and its aglycone, diosgenin, both suppressed the time-dependent increase of blood triacylglycerol level when orally injected with corn oil to mice, suggesting their inhibitory potential against fat absorption. Sprague-Dawley rats fed on a high-fat diet containing 5% Dioscorea nipponica Makino and 40% beef tallow gained significantly less body weight and adipose tissue than control animals fed on a high-fat diet alone during an 8-week experimental period (P<0.05).
authors.affiliation: Department of Food Science and Nutrition, Andong National University, S. Korea.
authors.forename: Chong-Suk
authors.initials: CS
authors.lastname: Kwon
authors.forename: Ho Yong
authors.initials: HY
authors.lastname: Sohn
authors.forename: Sung Hee
authors.initials: SH
authors.lastname: Kim
authors.forename: Ji Hyun
authors.initials: JH
authors.lastname: Kim
authors.forename: Kun Ho
authors.initials: KH
authors.lastname: Son
authors.forename: Jeong Soon
authors.initials: JS
authors.lastname: Lee
authors.forename: Jin Kyu
authors.initials: JK
authors.lastname: Lim
authors.forename: Jong-Sang
authors.initials: JS
authors.lastname: Kim
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects; D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001835:Body Weight / Q000187:drug effects; D004032:Diet; D004041:Dietary Fats / Q000008:administration & dosage / Q000009:adverse effects; D027742:Dioscorea / Q000737:chemistry*; D004144:Diosgenin / Q000031:analogs & derivatives* / Q000494:pharmacology; D004655:Emulsions / Q000008:administration & dosage; D005243:Feces / Q000737:chemistry; D008049:Lipase / Q000037:antagonists & inhibitors*; D008297:Male; D051379:Mice; D009765:Obesity / Q000097:blood / Q000188:drug therapy / Q000517:prevention & control*; D009929:Organ Size / Q000187:drug effects; D010179:Pancreas / Q000201:enzymology; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D014280:Triglycerides / Q000097:blood
chemical_list: D019440:Anti-Obesity Agents; D004041:Dietary Fats; D004655:Emulsions; D010936:Plant Extracts; D014280:Triglycerides; C019357:dioscin; D008049:Lipase; D004144:Diosgenin