title: Differential binding properties of B7-H1 and B7-DC to programmed death-1.
abstract: Programmed death-1 (PD-1) is a negative regulatory receptor expressed on activated T and B cells. Two ligands for PD-1, B7-H1 (PD-L1) and B7-DC (PD-L2), have been identified, but their binding properties have not been characterized yet. In this study, we generated soluble Ig fusion proteins of these molecules and examined the kinetics and relative affinities of the interactions between B7-H1 or B7-DC and PD-1 by flow cytometry and surface plasmon resonance. The interaction of B7-DC/PD-1 exhibited a 2-6-fold higher affinity and had different association/dissociation kinetics compared with the interaction of B7-H1/PD-1. Our results suggest that the differential binding properties of B7-H1 and B7-DC may be responsible for differential contributions of these two PD-1 ligands to immune responses.
authors.affiliation: Department of Molecular Immunology, Graduate School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan.
authors.forename: Pornpan
authors.initials: P
authors.lastname: Youngnak
authors.forename: Yuko
authors.initials: Y
authors.lastname: Kozono
authors.forename: Haruo
authors.initials: H
authors.lastname: Kozono
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Iwai
authors.forename: Noriko
authors.initials: N
authors.lastname: Otsuki
authors.forename: Hisayo
authors.initials: H
authors.lastname: Jin
authors.forename: Ken
authors.initials: K
authors.lastname: Omura
authors.forename: Hideo
authors.initials: H
authors.lastname: Yagita
authors.forename: Drew M
authors.initials: DM
authors.lastname: Pardoll
authors.forename: Lieping
authors.initials: L
authors.lastname: Chen
authors.forename: Miyuki
authors.initials: M
authors.lastname: Azuma
mesh_terms: D000818:Animals; D015703:Antigens, CD; D000954:Antigens, Surface / Q000235:genetics / Q000378:metabolism*; D051017:Apoptosis Regulatory Proteins; D018122:B7-1 Antigen / Q000235:genetics / Q000378:metabolism*; D060890:B7-H1 Antigen; D001798:Blood Proteins; D005434:Flow Cytometry; D006801:Humans; D019169:Jurkat Cells; D007700:Kinetics; D008024:Ligands; D008562:Membrane Glycoproteins; D051379:Mice; D010455:Peptides; D061027:Programmed Cell Death 1 Ligand 2 Protein; D061026:Programmed Cell Death 1 Receptor; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D020349:Surface Plasmon Resonance; D014407:Tumor Cells, Cultured
chemical_list: D015703:Antigens, CD; D000954:Antigens, Surface; D051017:Apoptosis Regulatory Proteins; D018122:B7-1 Antigen; D060890:B7-H1 Antigen; D001798:Blood Proteins; C423236:CD274 protein, human; C498919:Cd274 protein, mouse; D008024:Ligands; D008562:Membrane Glycoproteins; C105992:PDCD1 protein, human; C556977:PDCD1LG2 protein, human; C491383:Pdcd1 protein, mouse; C556978:Pdcd1lg2 protein, mouse; D010455:Peptides; D061027:Programmed Cell Death 1 Ligand 2 Protein; D061026:Programmed Cell Death 1 Receptor; D011993:Recombinant Fusion Proteins