Differential expression of sphingolipids in P-glycoprotein or multidrug resistance-related protein 1 expressing human neuroblastoma cell lines.
The sphingolipid composition and multidrug resistance status of three human neuroblastoma cell lines were established. SK-N-FI cells displayed high expression and functional (efflux) activity of P-glycoprotein, while multidrug resistance-related protein 1 was relatively abundant and most active in SK-N-AS cells. These two cell lines exhibited higher sphingolipid levels, compared to SK-N-DZ, which had the lowest activity of either ATP-binding cassette transporter protein. SK-N-DZ cells also differed in ganglioside composition with predominant expression of b-series gangliosides. In conclusion, these three neuroblastoma cell lines offer a good model system to study sphingolipid metabolism in relation to ATP-binding cassette transporter protein function.
Dijkhuis
Anne-Jan
AJ
Department of Membrane Cell Biology, Groningen University Institute for Drug Exploration (GUIDE), University of Groningen, A. Deusinglaan 1, Bldg. 3215, 10th Fl., 9713 AV, Groningen, The Netherlands. a.dijkhuis@med.rug.nl
Douwes
Jenny
J
Kamps
Willem
W
Sietsma
Hannie
H
Kok
Jan Willem
JW
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism* / Q000502:physiology; D000970:Antineoplastic Agents / Q000493:pharmacokinetics; D019008:Drug Resistance, Neoplasm; D005732:Gangliosides / Q000032:analysis; D006801:Humans; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000378:metabolism* / Q000502:physiology; D009447:Neuroblastoma / Q000473:pathology*; D013107:Sphingolipids / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000970:Antineoplastic Agents; D005732:Gangliosides; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D013107:Sphingolipids; C421109:multidrug resistance-associated protein 1