title: Structure of the biological repeating unit of the O-antigen of Pseudomonas aeruginosa immunotype 4 containing both 2-acetamido-2,6-dideoxy-D-glucose and 2-acetamido-2,6-dideoxy-D-galactose.
abstract: A phosphorylated core-lipid A backbone oligosaccharide that carries a disaccharide remainder of the first O-antigen repeating unit was derived by strong alkaline degradation following mild hydrazinolysis of the lipopolysaccharide of Pseudomonas aeruginosa immunotype 4 (serogroup O-1). The structure of the oligosaccharide was determined using ESI MS and NMR spectroscopy and it was demonstrated that 2-acetamido-2,6-dideoxy-D-glucose is the first monosaccharide of the O-polysaccharide that is linked to the LPS core. These data define the structure of the biological repeating unit of the O-antigen.
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors.forename: Olga V
authors.initials: OV
authors.lastname: Bystrova
authors.forename: Buko
authors.initials: B
authors.lastname: Lindner
authors.forename: Herman
authors.initials: H
authors.lastname: Moll
authors.forename: Nina A
authors.initials: NA
authors.lastname: Kocharova
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
authors.forename: Ulrich
authors.initials: U
authors.lastname: Zähringer
authors.forename: Gerald B
authors.initials: GB
authors.lastname: Pier
mesh_terms: D000117:Acetylglucosamine / Q000031:analogs & derivatives* / Q000737:chemistry*; D002240:Carbohydrate Sequence; D005643:Fucose / Q000031:analogs & derivatives* / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000737:chemistry / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000276:immunology
chemical_list: D008070:Lipopolysaccharides; D019081:O Antigens; D005643:Fucose; C059377:N-acetylfucosamine; C070625:2-acetamido-2,6-dideoxyglucose; D000117:Acetylglucosamine