[Factors affecting the determination of the percent carboxyhemoglobin saturation of blood].
OBJECTIVE
To investigate factors affecting the determination of the percent carboxyhemoglobin saturation (HbCO%) of blood in an attempt to offer further data for results interpretation and sample storage requirement.


METHODS
The HbCO% of blood samples stored in various conditions were detected by three spectrophotometries during the succeeding 30 days.


RESULTS
The data detected by reductive double-wavelength spectrophotometry and double-wavelength spectrophotometry were more stable than mono-wavelength spectrophotometry. The HbCO% of blood was significantly related with the storage conditions which include temperature, time and the degree of exposure to air.


CONCLUSIONS
Determinations of HbCO% are reliable which performed by reductive double-wavelength spectrophotometry and double-wavelength spectrophotometry, combine with spectral scans. During 30 days, blood stored at 4 degrees C exposed to a limited volume of air does not influence the determination of HbCO%.
Jiang
Yan
Y
Department of Forensic Medicine, Medical Center of Fudan University, Shanghai 200032, China.
Ye
Yong-hong
YH
Zhang
Yi-fan
YF
D002249:Carbon Monoxide Poisoning / Q000097:blood / Q000175:diagnosis*; D002263:Carboxyhemoglobin / Q000032:analysis*; D005554:Forensic Medicine; D006801:Humans; D013053:Spectrophotometry / Q000379:methods; D013696:Temperature
D002263:Carboxyhemoglobin