The thiocarbamate-inducible Rhodococcus enzyme ThcF as a member of the family of alpha/beta hydrolases with haloperoxidative side activity.
Purified thiocarbamate-inducible ThcF of Rhodococcus erythropolis NI86/21, overexpressed in Escherichia coli, displayed several characteristics of the HASH family of enzymes that groups prokaryotic proteins of the alpha/beta hydrolase superfamily possessing serine-dependent hydrolase and/or haloperoxidase activity. Kinetic analysis of bromination and ester hydrolysis revealed a low affinity of ThcF for model substrates. Sulfoxidation of thiocarbamates was demonstrated but probably represents a side activity due to peroxoacid generation by the enzyme. The thcF-linked thcG gene, encoding a LAL-type regulator, triggers expression of thcF in Rhodococcus. The tandem gene organization thcG-thcF is conserved in the thiocarbamate-degrading strain Rhodococcus sp. B30. It is proposed that HASH enzymes may be involved in the metabolism of plant-derived compounds.
De Mot
René
R
Department of Applied Plant Sciences, Catholic University of Leuven, Kasteelpark Arenberg 20, B-3001 Heverlee, Belgium. rene.demot@agr.kuleuven.ac.be
De Schrijver
Adinda
A
Schoofs
Geert
G
Parret
Annabel H A
AH
D004950:Esterases / Q000235:genetics / Q000378:metabolism; D006540:Herbicides / Q000378:metabolism; D006867:Hydrolases / Q000235:genetics* / Q000378:metabolism*; D007700:Kinetics; D010544:Peroxidases / Q000378:metabolism*; D010802:Phylogeny; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012240:Rhodococcus / Q000201:enzymology* / Q000235:genetics*; D012988:Soil Microbiology; D013859:Thiocarbamates / Q000378:metabolism*
D006540:Herbicides; D011994:Recombinant Proteins; D013859:Thiocarbamates; D010544:Peroxidases; C026195:bromide peroxidase; D006867:Hydrolases; D004950:Esterases