title: A novel morphological technique to investigate a single climbing fibre synaptogenesis with a Purkinje cell in the developing mouse cerebellum: DiI injection into the inferior cerebellar peduncle.
abstract: To study morphologically the relationship between climbing fibre and Purkinje cell in the developing mouse cerebellum, we established a novel tract tracing using injection of 1,1'-dioctodecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) into the inferior cerebellar peduncle, the half point of olivocerebellar projection. In this tracing method, only a certain number of climbing fibres were labelled with DiI and they revealed the single-fibre resolution, individually. These technical advantages enabled us to follow the projection of a climbing fibre to a Purkinje cell at the light microscopic level. To further investigate how a single labelled olivocerebellar axon interacts with a Purkinje cell, we introduced a photoconversion method into this tracing method and successfully observed the photo-oxidized climbing fibre terminals at the electron microscopic level. At postnatal days 7 and 9, a single DiI-labelled climbing fibre arborized around some adjacent Purkinje cell bodies in a distinguishable nest. At this pericellular nest stage, we first demonstrated that the terminal arborization stemmed from a single climbing fibre formed synapses simultaneously on both a soma and dendrites of a Purkinje cell. This finding suggests that the pericellular nest may be such an efficient form that a single climbing fibre innervates a Purkinje cell at both perisomatic and peridendritic sites. Thus, we succeeded in establishing an effective tracing method to investigate a single climbing fibre synaptogenesis with a Purkinje cell both at the light and electron microscopic levels.
authors.affiliation: Department of Biological Sciences, Graduate School of Medicine, Kyoto University, Yoshida-konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Yoshimoto
authors.initials: Y
authors.lastname: Kiyohara
authors.forename: Katsuaki
authors.initials: K
authors.lastname: Endo
authors.forename: Chizuka
authors.initials: C
authors.lastname: Ide
authors.forename: Akira
authors.initials: A
authors.lastname: Mizoguchi
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002232:Carbocyanines / Q000378:metabolism*; D002531:Cerebellum / Q000254:growth & development* / Q000648:ultrastructure; D005456:Fluorescent Dyes / Q000378:metabolism; D051379:Mice; D008854:Microscopy, Electron / Q000379:methods; D008856:Microscopy, Fluorescence; D009412:Nerve Fibers / Q000502:physiology / Q000648:ultrastructure*; D011689:Purkinje Cells / Q000502:physiology / Q000648:ultrastructure*; D013569:Synapses / Q000502:physiology*
chemical_list: D002232:Carbocyanines; D005456:Fluorescent Dyes; C024286:3,3'-dioctadecylindocarbocyanine