Genetic susceptibility to enteroaggregative Escherichia coli diarrhea: polymorphism in the interleukin-8 promotor region.
Enteroaggregative Escherichia coli (EAEC) infection can be identified in 26% of travelers with diarrhea and is associated with fecal interleukin (IL)-8 production. We hypothesized that single-nucleotide polymorphisms (SNPs) in the IL-8 gene are associated with EAEC-related symptoms. Fecal IL-8 production and IL-8 SNPs at 5 loci were identified in 69 US students who remained in Mexico for 5 weeks; 23 subjects had EAEC-associated diarrhea, 7 were asymptomatic EAEC carriers, 22 had nonspecific diarrhea, and 17 were asymptomatic without an enteropathogen. The chances of having EAEC-associated diarrhea were significantly increased among those with the AA genotype at the -251 position (odds ratio [OR], 208.51; 95% confidence interval [CI], 28.5-1525.36) and among those with AT genotype (OR, 14.3; 95% CI, 1.98-105.74), compared with those with the TT genotype at the -251 position. Among subjects with EAEC-associated diarrhea, the AA genotype at the -251 position produced greater concentrations of fecal IL-8 than those with the AT or TT genotype (P=.0053). In the present study, the AA genotype at the -251 position was associated with the occurrence of EAEC-associated diarrhea and increased levels of fecal IL-8.
Jiang
Zhi-Dong
ZD
University of Texas School of Public Health, Houston, Texas, USA. zjiang@sph.uth.tmc.edu.
Okhuysen
Pablo C
PC
Guo
Dong-Chuan
DC
He
Rumin
R
King
Terri M
TM
DuPont
Herbert L
HL
Milewicz
Dianna M
DM
D003967:Diarrhea / Q000150:complications / Q000235:genetics* / Q000382:microbiology*; D004926:Escherichia coli / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000150:complications* / Q000235:genetics*; D005243:Feces / Q000737:chemistry; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D016209:Interleukin-8 / Q000032:analysis / Q000235:genetics*; D008800:Mexico; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D013334:Students; D014195:Travel; D014481:United States / Q000208:ethnology
D016209:Interleukin-8