Mono(ADP-ribosyl)ation of DNA by apoptosis-inducing protein, pierisin.
The cabbage butterfly contains a potent cytotoxic protein, pierisin-1, and this protein is suggested to be an ADP-ribosylating toxin, Pierisin-1 effectively transferred an ADP-ribosyl group to DNA, but not to protein, as is the case with other bacteria-derived ADP-ribosylating toxins. Several spectral analyses and independent syntheses indicated that the acceptor site for ADP-ribosylation is N-2 of guanine base. Pierisin-1 induced apoptosis in mammalian cells accompanied by a release of cytochrome c and activation of a variety of caspases, and this apoptosis was inhibited by overexpression of Bcl-2. Pierisin-1 would be a novel DNA-damaging protein.
Watanabe
Masahiko
M
Cancer Prevention Division, National Cancer Center Research Institute, Tsukiji, Chuo-ku, Tokyo 104-0045, Japan.
Takamura-Enya
Takeji
T
Kanazawa
Takashi
T
Totsuka
Yukari
Y
Matsushima-Hibiya
Yuko
Y
Koyama
Kotaro
K
Sugimura
Takashi
T
Wakabayashi
Keiji
K
D036002:ADP Ribose Transferases; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D004247:DNA / Q000378:metabolism*; D019476:Insect Proteins / Q000378:metabolism* / Q000502:physiology
D019476:Insect Proteins; C113557:pierisin protein, insect; D000246:Adenosine Diphosphate Ribose; D004247:DNA; D036002:ADP Ribose Transferases