Development of an effective sample preparation method for the proteome analysis of body fluids using 2-D gel electrophoresis.
A sample preparation is still the most critical step in two-dimensional electrophoresis (2-DE) and should be optimized for each type of sample. In this study, a protein extraction method from body fluids was developed using a combined centrifugal filter device and a sample treating buffer. When plasma, amniotic fluid, urine, and tear were tested with this method, the recovery of protein reached almost 90% and high-quality separation of 2-DE gel was obtained.
Joo
Won-A
WA
Graduate School of Life Sciences and Biotechnology, Korea University, Seoul.
Lee
Do-Youn
DY
Kim
Chan-Wha
CW
D000653:Amniotic Fluid / Q000737:chemistry; D001798:Blood Proteins / Q000032:analysis / Q000302:isolation & purification; D001826:Body Fluids / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006801:Humans; D020543:Proteome / Q000032:analysis* / Q000302:isolation & purification; D040901:Proteomics / Q000379:methods*; D012680:Sensitivity and Specificity; D013666:Tears / Q000737:chemistry; D014556:Urine / Q000737:chemistry
D001798:Blood Proteins; D020543:Proteome