Increased lipid peroxidation in the liver and kidney and their mitochondrial fractions following buthionine sulfoximine induced glutathione depletion in guinea pigs.
Malondialdehyde (MDA) and diene conjugates (DC) and vitamin C levels and the activities of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) were determined in the liver and kidney and their mitochondrial fractions of guinea pigs 48 h after the injection of L-buthionine-(S,R)-sulfoximine (BSO), a glutathione (GSH) depleting agent. In BSO-induced GSH depletion, lipid peroxidation and SOD activities were found to be increased but GSH-Px activities did not change in the liver and kidney and their mitochondrial fractions. In addition, vitamin C levels remained unchanged in the liver and kidney homogenates. These results indicate that GSH depletion may influence oxidative stress in the liver and kidney and their mitochondrial fractions of guinea pigs.
Seçkin
S
S
Department of Biochemistry, Istanbul Faculty of Medicine, University of Istanbul, 34390 Istanbul, Turkey.
Doğru-Abbasoğlu
S
S
Başaran-Küçükgergin
C
C
Yavu
E
E
Cevikbaş
U
U
Aykaç-Toker
G
G
Uysal
M
M
D000818:Animals; D019328:Buthionine Sulfoximine / Q000633:toxicity*; D004791:Enzyme Inhibitors / Q000633:toxicity*; D005978:Glutathione / Q000172:deficiency*; D005979:Glutathione Peroxidase / Q000378:metabolism*; D006168:Guinea Pigs; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013482:Superoxide Dismutase / Q000378:metabolism
D004791:Enzyme Inhibitors; D008315:Malondialdehyde; D019328:Buthionine Sulfoximine; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione