Inhibition of FGF receptor signalling in Xenopus oocytes: differential effect of Grb7, Grb10 and Grb14.
The role of Grb7 adapters, Grb7, Grb10, and Grb14, was investigated in Xenopus oocytes expressing fibroblast growth factor receptors (FGFR). FGF-induced maturation of FGFR-expressing oocytes was blocked by previous injection of Grb7 or Grb14, but not Grb10. This effect correlated with Grb7/14 binding to the receptor, and inhibition of the Ras-dependent pathway. Interestingly, the phosphorylated insulin receptor interacting region (PIR) and Src 2 homology domains (SH2) of Grb7 and Grb14 were differently implicated in the inhibition of FGFR signalling. This study provided further evidence for specificity of the biological action of the Grb7 adapters on receptor tyrosine kinase signalling.
Cailliau
Katia
K
Université des Sciences; Technologies de Lille, Laboratoire de Biologie du Développement, UPRES UA 1033, IFR 118, Bâtiment SN3, Villeneuve d'Ascq, Cedex, France.
Le Marcis
Véronique
V
Béréziat
Véronique
V
Perdereau
Dominique
D
Cariou
Bertrand
B
Vilain
Jean Pierre
JP
Burnol
Anne-Françoise
AF
Browaeys-Poly
Edith
E
D000818:Animals; D002454:Cell Differentiation; D051383:GRB10 Adaptor Protein; D051382:GRB7 Adaptor Protein; D007328:Insulin / Q000494:pharmacology; D009865:Oocytes; D017434:Protein Structure, Tertiary / Q000502:physiology; D011506:Proteins / Q000502:physiology; D020794:Receptor Protein-Tyrosine Kinases; D051496:Receptor, Fibroblast Growth Factor, Type 1; D051499:Receptor, Fibroblast Growth Factor, Type 4; D017468:Receptors, Fibroblast Growth Factor / Q000037:antagonists & inhibitors*; D015398:Signal Transduction; D014981:Xenopus; D029867:Xenopus Proteins; D018909:src Homology Domains / Q000502:physiology
C490978:Grb14 protein, Xenopus; D007328:Insulin; D011506:Proteins; D017468:Receptors, Fibroblast Growth Factor; D029867:Xenopus Proteins; D051382:GRB7 Adaptor Protein; D051383:GRB10 Adaptor Protein; D020794:Receptor Protein-Tyrosine Kinases; D051496:Receptor, Fibroblast Growth Factor, Type 1; D051499:Receptor, Fibroblast Growth Factor, Type 4