[Construction of eucaryotic expression plasmid carrying the BMP7 gene and expression in mesenchymal stem cells].
OBJECTIVE
To construct an eucaryotic expression plasmid carrying the BMP7 gene and express in MSCs.


METHODS
The BMP7 gene was cloned into the eucaryotic expression vector pcDNA3.1. At the same time, mesenchymal stem cells (MSCs) were isolated and cultured in vitro. The plasmid carrying the BMP7 gene was transfected into MSCs.


RESULTS
PCR and digesting demonstrated that the eucaryotic expression plasmid -pcDNA-BMP7 was obtained. RT-PCR and immunohistochemical methods showed that the BMP7 gene was expressed in MSCs.


CONCLUSIONS
Construction of an eucaryotic expression plasmid carrying BMP7 gene and expression in MSCs provide a sound basis for gene therapy using the BMP7 gene and the ideal seeds for tissue engineering.
Hou
Shu-xun
SX
Department of Orthopeadics, 304th Hospital, People's Liberation Army, Beijing 100037, China.
Sun
Da-ming
DM
Du
Gui-xin
GX
Tong
Yi-gang
YG
Fu
Xiao-bing
XB
D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins / Q000235:genetics*; D015316:Genetic Therapy; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D010957:Plasmids; D016133:Polymerase Chain Reaction; D023822:Tissue Engineering; D016212:Transforming Growth Factor beta
C525819:BMP7 protein, human; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D016212:Transforming Growth Factor beta