Electrostatic complexation and photoinduced electron transfer between Zn-cytochrome c and [olyanionic fullerene dendrimers.
Two dendritic fullerene (DF) monoadducts, 2 and 3, which can carry up to 9 and 18 negative charges, respectively, were examined with respect to electrostatic complexation with Cytochrome c (Cytc). To facilitate comprehensive photophysical investigations, the zinc analogue of Cytc (ZnCytc) was prepared according to a novel, modified procedure. The association of ZnCytc and DF, and consequential photoinduced electron transfer within ZnCytc-DF from the photoexcited protein to the fullerene, was proven by fluorescence spectroscopy and transient absorption spectroscopy. These findings were also supported by circular dichroism as well as by extensive molecular dynamics (MD) simulations.
Braun
Martin
M
Institut für Organische Chemie Universität Erlangen-Nürnberg Henkestrasse 42, 91054 Erlangen, Germany.
Atalick
Stefan
S
Guldi
Dirk M
DM
Lanig
Harald
H
Brettreich
Michael
M
Burghardt
Stephan
S
Hatzimarinaki
Maria
M
Ravanelli
Elena
E
Prato
Maurizio
M
van Eldik
Rudi
R
Hirsch
Andreas
A
D000838:Anions / Q000737:chemistry*; D045304:Cytochromes c / Q000737:chemistry*; D004563:Electrochemistry; D004579:Electron Transport; D037741:Fullerenes / Q000737:chemistry*; D006418:Heme / Q000737:chemistry; D006860:Hydrogen Bonding; D008956:Models, Chemical; D008958:Models, Molecular; D015394:Molecular Structure; D010777:Photochemistry; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013055:Spectrophotometry, Infrared; D055672:Static Electricity; D013237:Stereoisomerism; D015032:Zinc / Q000737:chemistry*
D000838:Anions; D037741:Fullerenes; D011108:Polymers; D006418:Heme; D045304:Cytochromes c; D015032:Zinc