Effects of sulfur mustard on transcription in human epidermal keratinocytes: analysis by mRNA differential display.
This study examines the transcriptional response of human epidermal keratinocytes (HEK) to sulfur mustard (HD) in order to gain a better understanding of the intracellular events that result in cytotoxicity. Differential display polymerase chain reaction technology was used to examine the relative transcriptional activity of healthy cells to those exposed to subvesicating or vesicating concentrations of HD for 4 h. Approximately 2% of the HEK transcriptome had altered expression. Sixty of the most prominently altered transcripts were characterized. Important upregulated genes include NADH dehydrogenase III, GADD45 and ubiquitin. Key downregulated genes include type I keratin 14, alpha-enolase and caltractin. Many of the identified transcripts protein products presently do not have an assigned function and eleven transcripts were unidentifiable. These transcriptional alterations provide one of the first molecular insights into the intracellular events induced by HD.
Platteborze
Peter L
PL
Pharmacology Division, USAMRICD, Aberdeen Proving Ground, MD 21010-5425, USA. Peter.Platteborze@det.amedd.army.mil
D002478:Cells, Cultured; D002619:Chemical Warfare Agents / Q000633:toxicity*; D020869:Gene Expression Profiling; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism; D009151:Mustard Gas / Q000633:toxicity*; D012333:RNA, Messenger / Q000187:drug effects* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000187:drug effects*
D002619:Chemical Warfare Agents; D012333:RNA, Messenger; D009151:Mustard Gas