RETRACTED: Reelin promotes peripheral synapse elimination and maturation.
Reelin is an extracellular protein that is crucial for layer formation in the embryonic brain. Here, we demonstrate that Reelin functions postnatally to regulate the development of the neuromuscular junction. Reelin is required for motor end-plate maturation and proper nerve-muscle connectivity, and it directly promotes synapse elimination. Unlike layer formation, neuromuscular junction development requires a function of Reelin that is not mediated by Disabled1 or very-low-density lipoprotein receptors and apolipoprotein E receptor 2 receptors but by a distinct mechanism involving its protease activity.
Quattrocchi
Carlo C
CC
The Cain Foundation Laboratories, Houston, TX 77030, USA.
Huang
Cheng
C
Niu
Sanyong
S
Sheldon
Michael
M
Benhayon
David
D
Cartwright
Joiner
J
Mosier
Dennis R
DR
Keller
Flavio
F
D'Arcangelo
Gabriella
G
D000200:Action Potentials; D000818:Animals; D001369:Axons / Q000378:metabolism; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D017077:Culture Media, Conditioned; D003964:Diaphragm / Q000294:innervation; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D026502:LDL-Receptor Related Proteins; D051379:Mice; D008818:Mice, Neurologic Mutants; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D009045:Motor Endplate / Q000648:ultrastructure; D009046:Motor Neurons / Q000378:metabolism; D018482:Muscle, Skeletal / Q000294:innervation; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009469:Neuromuscular Junction / Q000254:growth & development* / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D011973:Receptors, LDL / Q000235:genetics / Q000378:metabolism; D018110:Receptors, Lipoprotein / Q000235:genetics / Q000378:metabolism; D000091042:Reelin Protein; D012583:Schwann Cells / Q000378:metabolism; D012697:Serine Endopeptidases; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology; D013450:Sulfones / Q000494:pharmacology; D013569:Synapses / Q000502:physiology* / Q000648:ultrastructure
D015816:Cell Adhesion Molecules, Neuronal; D017077:Culture Media, Conditioned; C103773:Dab1 protein, mouse; D016326:Extracellular Matrix Proteins; D026502:LDL-Receptor Related Proteins; D009419:Nerve Tissue Proteins; D011973:Receptors, LDL; D018110:Receptors, Lipoprotein; D000091042:Reelin Protein; D015842:Serine Proteinase Inhibitors; D013450:Sulfones; C036053:VLDL receptor; C098773:low density lipoprotein receptor-related protein 8; C002010:4-(2-aminoethyl)benzenesulfonylfluoride; C000717128:Reln protein, mouse; D012697:Serine Endopeptidases