title: Immobilization of pectinmethylesterase from acerola (Malpighia glabra L.) in porous silica.
abstract: The total and partially purified enzyme pectinmethylesterase from acerola fruit was covalently immobilized on porous silica particles. These efficiency values were 114% for the total PME and 351% for the partially purified PME. In both forms the immobilization resulted in compounds with high thermal stability.
authors.affiliation: Instituto de Química, Departamento de Bioquímica e Tecnologia Química, UNESP, Rua Prof. Francisco Degni, s/n. o, C.P. 355, CEP 14801-970, Araraquara, SP, Brasil.
authors.forename: Sandra Aparecida
authors.initials: SA
authors.lastname: de Assis
authors.forename: Henrique Celso
authors.initials: HC
authors.lastname: Trevisan
authors.forename: Olga Maria
authors.initials: OM
authors.lastname: Mascarenhas
authors.forename: Faria
authors.initials: F
authors.lastname: Oliveira
mesh_terms: D002265:Carboxylic Ester Hydrolases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis* / Q000737:chemistry; D004789:Enzyme Activation; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D029581:Malpighiaceae / Q000737:chemistry / Q000201:enzymology*; D008567:Membranes, Artificial; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D013696:Temperature
chemical_list: D020099:Coated Materials, Biocompatible; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D012822:Silicon Dioxide; D002265:Carboxylic Ester Hydrolases; C022323:pectinesterase