title: Site-directed mutagenesis using the megaprimer method.
authors.affiliation: Department of Surgery, Comprehensive Cancer Center, University of California, San Francisco, USA.
authors.forename: Zhidong
authors.initials: Z
authors.lastname: Xu
authors.forename: Alessia
authors.initials: A
authors.lastname: Colosimo
authors.forename: Dieter C
authors.initials: DC
authors.lastname: Gruenert
mesh_terms: D011232:Chemical Precipitation; D003001:Cloning, Molecular; D017931:DNA Primers; D004274:DNA, Recombinant / Q000235:genetics / Q000302:isolation & purification; D000431:Ethanol; D016297:Mutagenesis, Site-Directed; D010957:Plasmids / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D004274:DNA, Recombinant; D000431:Ethanol