[The function of integrin beta 1 in the hypoxic cellular damage mechanism of renal tubular epithelial cell of newborn porcine].
OBJECTIVE
To inquire into the mechanism of renal deficiency in neonatal asphyxia.


METHODS
The 30, 60, 90, 120 min hypoxic renal cell model was made by use of DMEM/F-12 with antimycine A. ATP content was detected by reversed-phase HPLC. The levels of integrin beta 1 of renal tubular epithelial(RTE) cell of the groups were detected using flow cytometry.


RESULTS
ATP deficiency cellular model of newborn porcine RTE cell was successfully set up. The levels of ATP of the newborn porcine RET cell during 30, 60, 90, 120 min hypoxia time significantly decreased as compared with normal control (P < 0.001). The levels of integrin beta 1 of newborn porcine RTE cell decreased as the ATP deficiency time increased and the differences between the groups were statistically significant (P < 0.001).


CONCLUSIONS
The decreased level of integrin beta 1 in the newborn porcine RTE cell during anoxia may play an important role in the newborn renal deficiency during asphyxia.
Chen
Juan
J
Department of Pediatrics, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Yao
Yujia
Y
Xiong
Ying
Y
Chen
Dapeng
D
Chen
Yongxiu
Y
D000818:Animals; D000831:Animals, Newborn; D001237:Asphyxia / Q000378:metabolism; D015687:Cell Hypoxia; D019012:Integrin beta1 / Q000378:metabolism*; D007684:Kidney Tubules / Q000166:cytology / Q000378:metabolism*; D013552:Swine
D019012:Integrin beta1