title: RNA LEGO: magnesium-dependent assembly of RNA building blocks through loop-loop interactions.
abstract: We describe the construction of nano-molecular assemblies using RNA building blocks the human immunodeficiency virus type 1 (HIV-1) dimerization initiation site (DIS) RNA, that forms stable base pairing through a magnesium-dependent loop-loop interaction ("kissing"). RNA building blocks containing two DIS or DIS-like hairpins connected by a two nucleotide linker self-assembled to form specific structures as observed by non-denaturing polyacrylamide gel electrophoresis (PAGE). Furthermore, observation of "real time" formation of the molecular assemblies by circular dichroism (CD) spectroscopy was attempted.
authors.affiliation: Department of Life Science, Tokyo Gakugei University, Koganei, Tokyo 184-8501, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Horiya
authors.forename: Xianglan
authors.initials: X
authors.lastname: Li
authors.forename: Gota
authors.initials: G
authors.lastname: Kawai
authors.forename: Ryota
authors.initials: R
authors.lastname: Saito
authors.forename: Akira
authors.initials: A
authors.lastname: Katoh
authors.forename: Koh
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Kazuo
authors.initials: K
authors.lastname: Harada
mesh_terms: D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry*
chemical_list: D012313:RNA