title: Aminoacylation of tRNA by antisense molecule.
abstract: Aminoacylation of tRNA was attempted through formation of tRNA/DNA/aa-PNA (N-aminoacylated peptide nucleic acid) ternary hybrid. A 23-mer DNA, that is complementary to a 3'-terminal of tRNA and to a 9-mer PNA carrying an amino acid unit, was designed to achieve close proximity between the amino acid and the 3'-OH group of tRNA. The aminoacylation was carried out in a buffer solution containing imidazole. The aminoacylation was detected by nuclease S1 treatment followed by HPLC and MALDI-TOF MS. This novel methodology will open a way for easy and versatile aminoacylation of nonnatural amino acids onto specific tRNAs.
authors.affiliation: Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, Okayama, 700-8530, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Ninomiya
authors.forename: Takamasa
authors.initials: T
authors.lastname: Endo
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Minohata
authors.forename: Masahisa
authors.initials: M
authors.lastname: Tajiri
authors.forename: Maya
authors.initials: M
authors.lastname: Suzuki
authors.forename: Tomoyoshi
authors.initials: T
authors.lastname: Kurita
authors.forename: Takahiro
authors.initials: T
authors.lastname: Hohsaka
authors.forename: Masahiko
authors.initials: M
authors.lastname: Sisido
mesh_terms: D000215:Acylation; D002021:Buffers; D002851:Chromatography, High Pressure Liquid; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D012343:RNA, Transfer / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D002021:Buffers; D016376:Oligonucleotides, Antisense; D012343:RNA, Transfer