title: Location and function of intrahepatic shunts in anaesthetised rats.
abstract: BACKGROUND
  In the present study we determined the proportion of shunt flow due to patent intrahepatic portal systemic shunts in the normal rat liver and its relationship with microsphere induced portal hypertension.
  
  
  METHODS
  Systemic and hepatic haemodynamics were measured continuously before, during, and after intraportal injection of 15 micro m diameter microspheres in anaesthetised male Wistar rats. Functional hepatic blood flow and intrahepatic shunt flow were determined by the use of constant intraportal infusion of sorbitol and simultaneous measurements in the portal vein, hepatic vein, and carotid artery. The percentage of large shunts of diameter >15 micro m were estimated by intraportal injection of (51)Cr labelled 15 micro m diameter microspheres.
  
  
  RESULTS
  Hepatic sorbitol uptake was 97.9 (0.5)% in normal control rats, with functional hepatic blood flow equalling total hepatic blood flow (2.52 (0.23) ml/min/100 g body weight). Microsphere injection decreased sorbitol uptake to 12.8 (4.3)% and further to 4.1 (0.7)% when followed by hepatic arterial ligation. In the latter two groups, intrahepatic shunt flow (1.46 (0.15) and 1.16 (0.19) ml/min/100 g body weight, respectively) was not significantly different from portal venous flow (1.36 (0.20) and 1.20 (0.20) ml/min/100 g body weight, respectively). Portal venous flow remained at 70% of basal values and portal venous pressure only increased by 50% from baseline. (51)Cr labelled microsphere shunt fraction through large shunts (>15 micro m) was less than 1.0%.
  
  
  CONCLUSIONS
  The site of confluence between the hepatic artery and portal vein is in zone II. Intrahepatic shunts originate in presinusoidal regions in zone I in the normal liver and, when activated by intraportal injection of microspheres, divert 70% of the total portal blood flow away from zone III and thereby reduce acute increases in portal venous pressure.
authors.affiliation: Liver Sciences Unit, Academic Department of Surgery, St Thomas's Hospital, Guy's, King's, and St Thomas's School of Medicine, London, UK.
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: I S
authors.initials: IS
authors.lastname: Benjamin
authors.forename: R
authors.initials: R
authors.lastname: Naftalin
authors.forename: B
authors.initials: B
authors.lastname: Alexander
mesh_terms: D000818:Animals; D002339:Carotid Arteries / Q000378:metabolism; D006439:Hemodynamics / Q000502:physiology; D006503:Hepatic Veins / Q000378:metabolism; D006975:Hypertension, Portal / Q000378:metabolism*; D008297:Male; D008863:Microspheres; D011169:Portal Vein / Q000378:metabolism; D011170:Portasystemic Shunt, Surgical / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D013012:Sorbitol / Q000008:administration & dosage / Q000378:metabolism*
chemical_list: D013012:Sorbitol