[Apoptosis of cancer cells induced by influenza virus and its elementary mechanism].
OBJECTIVE
To study the apotosis-inducing effects of influenza virus on cancer cells and the role of FasL.


METHODS
Four strains of cancer cells (Hela, Raji, SMMC-7721 and SPC-A-1) were infected with 20 m. o. i influenza virus and the effects of apoptosis were detected at different time points post infection by electron microscopy, DNA agrose gel electrophoresis, PI stained flow cytometry, and Annexin-V FITC/PI stained flow cytometry. And the concentration of FasL in the culture supernatant was measured with the use of ELISA at the same time.


RESULTS
All the four strains of cancer cells showed apoptotic morphological changes. DNA agarose electrophoresis also showed "ladder" in a time dependent manner after virus infection. During this course, there was an obvious increase of FasL concentration in the cell culture supernatant.


CONCLUSIONS
Influenza virus can induce tumour cells apoptosis in vitro, and it is probably related to the increased expression of FasL.
Li
Hong
H
Department of Microbiology, West China School of Preclinical and Forenisic Medicine, Sichuan University, Chengdu 610041, China.
Li
Hualin
H
Li
Wanyi
W
Xiao
Liying
L
Jiang
Zhonghua
Z
Li
Mingyuan
M
Wang
Xiaodong
X
Zhang
Lin
L
D000230:Adenocarcinoma / Q000473:pathology / Q000821:virology; D017209:Apoptosis; D053222:Fas Ligand Protein; D006367:HeLa Cells; D006801:Humans; D009980:Influenza A virus / Q000502:physiology*; D007942:Leukemia, Experimental / Q000473:pathology / Q000821:virology; D008113:Liver Neoplasms / Q000473:pathology / Q000821:virology; D008175:Lung Neoplasms / Q000473:pathology / Q000821:virology; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000235:genetics; D014407:Tumor Cells, Cultured
C505661:FASLG protein, human; D053222:Fas Ligand Protein; D008562:Membrane Glycoproteins