title: Construction of YAC/BAC contig map for the BTA 6q21 region containing a locus for bovine chondrodysplastic dwarfism.
abstract: To characterize the bovine chromosome 6q21 for bovine chondrodysplastic dwarfism (BCD), we developed 48 new microsatellite markers from yeast artificial chromosome (YAC) and bacterial artificial chromosome (BAC) clones using a modified magnetic bead capture method. These new markers were used to construct a high-resolution physical map of the region with a total of 85 loci. The physical map will be a powerful tool for successful positional cloning experiments.
authors.affiliation: Shirakawa Institute of Animal Genetics, Odakura, Nishigo, Fukushima, Japan. haruko@coccoa.ocn.ne.jp
authors.forename: Haruko
authors.initials: H
authors.lastname: Takeda
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Sugimoto
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000235:genetics*; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics*; D033481:Chromosomes, Mammalian / Q000235:genetics*; D020451:Contig Mapping; D005097:Exostoses, Multiple Hereditary / Q000235:genetics* / Q000662:veterinary*; D005819:Genetic Markers; D018895:Microsatellite Repeats / Q000235:genetics; D016324:Sequence Tagged Sites
chemical_list: D005819:Genetic Markers