title: Effect of carotenoid on calcium content and alkaline phosphatase activity in rat femoral tissues in vitro: the unique anabolic effect of beta-cryptoxanthin.
abstract: The effects of various carotenoids and rutin on calcium content and alkaline phosphatase activity in the femoral-diaphyseal and femoral-metaphyseal tissues of young rats in vitro were investigated. Bone tissues were cultured for 48 h in serum-free Dulbecco's modified Eagle's medium containing either vehicle or a compound (10(-8)-10(-6) M). The presence of beta-cryptoxanthin (10(-7) or 10(-6) M) caused a significant increase in calcium content and alkaline phosphatase activity in the femoral-diaphyseal and femoral-metaphyseal tissues. Also, the lowest concentration (10(-8) M) of beta-cryptoxanthin used caused a significant increase in diaphyseal and metaphyseal calcium content. Lutein (10(-8)-10(-6) M) had no effect on diaphyseal and metaphyseal calcium contents and diaphyseal alkaline phosphatase activity, while 10(-7) and 10(-6) M lutein significantly decreased metaphyseal alkaline phosphatase activity. Lycopene (10(-8)-10(-6) M) or rutin (10(-8)-10(-6) M) did not have a significant effect on bone calcium content and alkaline phosphatase activity. The present study suggests that the carotenoid beta-cryptoxanthin has a unique anabolic effect on bone calcification in vitro.
authors.affiliation: Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, Shizuoka, Japan. yamaguch@u-shizuoka-ken.ac.jp
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Yamaguchi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Uchiyama
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002338:Carotenoids / Q000494:pharmacology; D065366:Cryptoxanthins; D004305:Dose-Response Relationship, Drug; D005269:Femur / Q000187:drug effects* / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D024341:Xanthophylls; D019207:beta Carotene / Q000031:analogs & derivatives* / Q000494:pharmacology*
chemical_list: D065366:Cryptoxanthins; D024341:Xanthophylls; D019207:beta Carotene; D002338:Carotenoids; D000469:Alkaline Phosphatase; D002118:Calcium