title: Cytokine responses in mice infected with Clonorchis sinensis.
abstract: FVB and BALB/c mice show different morbidity, development of Clonorchis sinensis, and pathological changes following C. sinensis infection. FVB mice are susceptible and BALB/c mice are relatively more resistant to C. sinensis infection. To investigate the relationship between cytokine reaction and susceptibility to C. sinensis infection in FVB and BALB/c mice, we described both the patterns and kinetics of Th1 cytokines and Th2 cytokines in spleen cell culture. Interleukin (IL)-4 and IL-10 cytokine production in the culture supernatants of the concanavalin-A-stimulated spleen cells increased at 2-3 weeks post-infection in both strains. IL-5 production increased between 2 and 5 weeks post-infection in both strains, and reached a peak level at 2 weeks post-infection in BALB/c mice and 4 weeks post-infection in FVB mice. In contrast, gamma interferon (IFN-gamma) production decreased between 2 and 4 weeks in both strains. IL-2 production increased slightly in BALB/c mice following infection, but was unchanged in FVB mice. IL-4 production over preinfection levels was significantly higher in FVB mice, whereas IFN-gamma, IL-2, and IL-10 production were significantly higher in BALB/c mice. The levels of serum immunoglobulin E (IgE) and blood eosinophils in both mouse strains significantly increased between 3 and 6 weeks postinfection. Serum IgE levels were significantly higher in FVB mice than in BALB/c mice. The results of this study suggest that susceptibility to C. sinensis infection is associated with Th2 cytokine production, especially IL-4 which is predominant in relatively susceptible FVB mice.
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea.
authors.forename: Yang Kyu
authors.initials: YK
authors.lastname: Choi
authors.forename: Byung Il
authors.initials: BI
authors.lastname: Yoon
authors.forename: Young Suk
authors.initials: YS
authors.lastname: Won
authors.forename: Chul Ho
authors.initials: CH
authors.lastname: Lee
authors.forename: Byung Hwa
authors.initials: BH
authors.lastname: Hyun
authors.forename: Hyoung Chin
authors.initials: HC
authors.lastname: Kim
authors.forename: Goo Taeg
authors.initials: GT
authors.lastname: Oh
authors.forename: Dae Yong
authors.initials: DY
authors.lastname: Kim
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D002478:Cells, Cultured; D003003:Clonorchiasis / Q000276:immunology* / Q000469:parasitology / Q000473:pathology; D003004:Clonorchis sinensis / Q000254:growth & development / Q000276:immunology / Q000472:pathogenicity*; D016207:Cytokines / Q000096:biosynthesis*; D004198:Disease Susceptibility; D004804:Eosinophils / Q000276:immunology; D005260:Female; D007073:Immunoglobulin E / Q000097:blood; D008099:Liver / Q000469:parasitology / Q000473:pathology; D051379:Mice; D013154:Spleen / Q000166:cytology; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; D016207:Cytokines; D007073:Immunoglobulin E