Carbon-nanotube-modified glassy carbon electrodes for amplified label-free electrochemical detection of DNA hybridization.
The preparation and attractive performance of carbon-nanotube modified glassy-carbon (CNT/GC) electrodes for improved detection of purines, nucleic acids, and DNA hybridization are described. The surface-confined multiwall carbon-nanotube (MWCNT) facilitates the adsorptive accumulation of the guanine nucleobase and greatly enhances its oxidation signal. The advantages of CNT/GC electrodes are illustrated from comparison to the common unmodified glassy carbon, carbon paste and graphite pencil electrodes. The dramatic amplification of the guanine signal has been combined with a label-free electrical detection of DNA hybridization. Factors influencing the enhancement of the guanine signal are assessed and optimized. The performance characteristics of the amplified label-free electrochemical detection of DNA hybridization are reported in connection to measurements of nucleic-acid segments related to the breast-cancer BRCA1 gene.
Wang
Joseph
J
Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, New Mexico 88003, USA.
Kawde
Abdel-Nasser
AN
Musameh
Mustafa
M
D000818:Animals; D002417:Cattle; D004247:DNA / Q000032:analysis*; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D008863:Microspheres; D037742:Nanotubes, Carbon; D009693:Nucleic Acid Hybridization / Q000379:methods
D037742:Nanotubes, Carbon; D004247:DNA