title: Serum chitotriosidase activity is increased in subjects with atherosclerosis disease.
abstract: OBJECTIVE
  This study was undertaken to analyze the relation between serum activity of chitotriosidase enzyme, a protein synthesized exclusively by activated macrophages, and atherosclerotic lesion extent in subjects with atherothrombotic stroke (ATS) and in subjects with ischemic heart disease (IHD).
  
  
  RESULTS
  We assayed the serum chitotriosidase activity and a common chitotriosidase gene polymorphism that causes deficiency in chitotriosidase activity in 3 Spanish populations, ATS (n=153), IHD (n=124), and control (n=148) subjects. Statistical differences were found in serum chitotriosidase activity between ATS (88.1+/-4.6 nmol/mL. h, P<0.0001) and IHD subjects (79.0+/-6.3, P=0.002) versus control group (70.9+/-5.2). These observed differences were not attributable to a distinct allelic or genotype distribution. The extension of the atherosclerotic lesion in carotids of ATS subjects was measured by duplex sonography. Chitotriosidase activities were 66.9+/-9.6, 88.7+/-8.3, and 107.7+/-11.8 for subjects with carotid stenosis <or=30%, 31% to 60%, and >60%, respectively. Statistical differences were observed between subjects with major and intermediate stenosis grade compared with subjects with minor stenosis, P=0.005 and P=0.016, respectively.
  
  
  CONCLUSIONS
  Serum chitotriosidase activity is significantly increased in individuals suffering from atherosclerosis disease and is related to the severity of the atherosclerotic lesion, suggesting a possible role as atherosclerotic extent marker.
authors.affiliation: Laboratorio de Investigación Molecular, Hospital Universitario Miguel Servet, Isabel la Católica, 1-3, 50009 Zaragoza, Spain. martieda@hmservet.insalud.es
authors.forename: Marta
authors.initials: M
authors.lastname: Artieda
authors.forename: Ana
authors.initials: A
authors.lastname: Cenarro
authors.forename: Alberto
authors.initials: A
authors.lastname: Gañán
authors.forename: Ivonne
authors.initials: I
authors.lastname: Jericó
authors.forename: Carmen
authors.initials: C
authors.lastname: Gonzalvo
authors.forename: Juan M
authors.initials: JM
authors.lastname: Casado
authors.forename: Isabel
authors.initials: I
authors.lastname: Vitoria
authors.forename: José
authors.initials: J
authors.lastname: Puzo
authors.forename: Miguel
authors.initials: M
authors.lastname: Pocoví
authors.forename: Fernando
authors.initials: F
authors.lastname: Civeira
mesh_terms: D000368:Aged; D001161:Arteriosclerosis / Q000097:blood* / Q000201:enzymology* / Q000235:genetics; D015992:Body Mass Index; D002545:Brain Ischemia / Q000097:blood / Q000201:enzymology / Q000235:genetics; D002097:C-Reactive Protein / Q000378:metabolism; D006596:Hexosaminidases / Q000097:blood* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D011110:Polymorphism, Genetic / Q000235:genetics; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D013030:Spain; D020521:Stroke / Q000097:blood / Q000201:enzymology / Q000235:genetics
chemical_list: D002097:C-Reactive Protein; D006596:Hexosaminidases; C086400:chitotriosidase