Effects of dietary vitamin K levels on bone quality in broilers.
This study was conducted to evaluate the effects of dietary vitamin K (menadione) on bone quality in cage-raised broilers. Three hundred and sixty male broilers were randomly allotted to one of six treatments, with six replicate pens per treatment and 10 chicks per pen. Broilers were fed one of six diets including a control diet or the control diet plus graded levels of vitamin K (0.5 mg/kg, 2 mg/kg, 8 mg/kg, 32 mg/kg and 128 mg/kg). Water and feed were provided ad libitum during the 7-week experimental period. Results indicated that vitamin K supplementation of broilers diets significantly effected bone quality and feed efficiency. The treatment containing vitamin K at 8 mg/kg improved growth performance (during weeks 6-7) and bone quality (during weeks 0-3). In our study, hydroxyapatite binding capacity of serum osteocalcin (during weeks 0-3), bone breaking strength, bone flexibility, bone ash weight increased linearly (P < 0.05) and bone mineral density, bone mineral content increased quadratically (P < 0.05) with increasing supplementation of vitamin K. In conclusion, to gain optimum bone quality and broiler performance, our studies suggest that the concentration of vitamin K in broilers diets should be 8 mg/kg, 2 mg/kg, and 2 mg/kg, for the starter, grower and finisher phases, respectively. Furthermore, it was shown that the starter period is an important phase for improving bone quality. In addition, this study validated the mechanism of vitamin K effects on bone quality. Vitamin K boosts the carboxylation of osteocalcin and decreases the concentration of serum under-carboxylated osteocalcin enhancing hydroxyapatite binding capacity of serum osteocalcin and improving bone quality.
Zhang
Caiyun
C
National Feed Engineering Technology Research Center, China Agricultural University, Beijing, China.
Li
Defa
D
Wang
Fenglai
F
Dong
Tao
T
D000821:Animal Feed; D000818:Animals; D015519:Bone Density / Q000187:drug effects / Q000502:physiology; D001846:Bone Development; D001842:Bone and Bones / Q000378:metabolism*; D002113:Calcification, Physiologic / Q000187:drug effects* / Q000502:physiology; D002645:Chickens / Q000254:growth & development* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D008297:Male; D015675:Osteocalcin / Q000378:metabolism*; D011897:Random Allocation; D013718:Tensile Strength; D014812:Vitamin K / Q000008:administration & dosage* / Q000494:pharmacology
D015675:Osteocalcin; D014812:Vitamin K