[Detection of point mutation in p53 gene by capillary electrophoresis-PCR-SSCP analysis].
OBJECTIVE
To screen the point mutation of p53 gene rapidly by capillary electrophoresis (CE).


METHODS
A simple capillary electrophoresis system for the strand conformational polymorphism (SSCP) analysis of genomic DNA was developed by choosing commercially available capillary and gel buffer. This CE-SSCP system was applied to the analysis of twenty PCR products amplified from the exon 7 of the p53 gene of tissue specimens from patients with colon cancer.


RESULTS
Five of them (Ca4, Ca6, Ca7, Ca8, Ca14) were found to have mutation within 25 minutes, while the results of the SSCP analysis by PAGE-silver staining techniques only detected mutation of four samples. And sequencing of these PCR products proved that the results of CE-SSCP were correct.


CONCLUSIONS
This simple, accurate and less expensive method could be used in the field of medical research and in the clinical DNA diagnosis of human cancers and other diseases.
Liu
Ji
J
Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Jing
Huie
H
Ru
Qinhua
Q
Deng
Yingping
Y
Luo
Guoan
G
Huang
Qian
Q
D001483:Base Sequence; D003110:Colonic Neoplasms / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D019075:Electrophoresis, Capillary; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational
D004273:DNA, Neoplasm