Role of 2'-OH on thermodynamic stability and structure of chimeric oligonucleotides.
Thermodynamic parameters of RNA/DNA chimeric duplexes were obtained systematically. Stability of the duplexes including chimeric junctions was context dependent, although CD spectra showed less structural perturbation by the junctions. Nearest-neighbor delta G degree 37 values for these chimeric RNA-DNA junctions suggested that 2'-OH on the 5'-side nucleotide at the junction may be the determinant for the nearest-neighbor stability of the chimeric junction.
Kanzaki
Takayuki
T
Department of Chemistry, Faculty of Science and Engineering, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan.
Nakano
Shu-ichi
S
Sugimoto
Naoki
N
D001483:Base Sequence; D002678:Chimera; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D009841:Oligonucleotides / Q000737:chemistry*; D013816:Thermodynamics
D009841:Oligonucleotides