Direct monitoring of EcoRII restriction enzyme reactions on a 27 MHz quartz-crystal microbalance.
EcoRII is a type IIe restriction endonuclease characterized by a highly cooperative reaction mechanism that depends on simultaneous binding of the dimeric enzyme molecule to two copies of its recognition site. Gaining insights about reaction property of EcoRII binding to its recognition site and effect of divalent metal ion on DNA cleavage process, a DNA-immobilized quartz crystal microbalance (QCM), which enables real-time monitoring both the binding of enzyme and the cleavage reaction on DNA strands as mass changes, was utilized.
Takahashi
Shuntaro
S
Department of Biomolecular Engineering, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.
Matsuno
Hisao
H
Furusawa
Hiroyuki
H
Okahata
Yoshio
Y
D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D011791:Quartz
D011791:Quartz; C046545:CCWGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific