Long-term viability and infectivity of intraradical forms of Glomus intraradices vesicles encapsulated in alginate beads.
Intraradical vesicles of Glomus intraradices were isolated, entrapped in alginate beads, and stored at 4 degrees C for periods from 2-74 months. The beads were used to inoculate leeks grown under standard conditions for 6 wk, then development of root colonization by G. intraradices was recorded. Colonization of leeks was high (mean > 50% in length) and did not vary markedly until five years of storage. After six years of storage, the inoculum proved infective and viable.
Plenchette
Christian
C
Institut Nationale de Recherche Agronomique, Unité de Malherbologie et Agronomie, 17 rue Sully, 21065 Dijon Cedex, France. christian.plenchette@dijon.inra.fr
Strullu
Désiré G
DG
D000464:Alginates; D018914:Cells, Immobilized; D003080:Cold Temperature; D005658:Fungi / Q000254:growth & development* / Q000648:ultrastructure*; D025301:Hyphae / Q000254:growth & development; D008863:Microspheres; D009172:Mycology / Q000379:methods; D038821:Mycorrhizae / Q000254:growth & development; D019697:Onions / Q000382:microbiology*; D013997:Time Factors
D000464:Alginates