Monitoring the cellular activity of a cultured single cell by scanning electrochemical microscopy (SECM). A comparison with fluorescence viability monitoring.
The respiratory activities of cultured HeLa cells were monitored at a single cell level using scanning electrochemical microscopy (SECM) that produces images of the localized distribution of oxygen around the cell. The change in the cellular activity was traced after exposures to KCN, ethyl alcohol and the antibiotic drug, Antimycin A. The results were compared with those from the conventional fluorescence monitoring using Calcein-AM that is sensitive to deformation of the cell membrane. The SECM-based measurement follows the decrease in the cellular activity upon exposure to KCN and Antimycin A more rapidly than the fluorescence-based measurements, demonstrating that SECM is suitable for studying the cellular influence of respiration inhibitors.
Kaya
Takatoshi
T
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, 07 Aramaki-aoba, 980-8579, Sendai, Japan
Torisawa
Yu-suke
YS
Oyamatsu
Daisuke
D
Nishizawa
Matsuhiko
M
Matsue
Tomokazu
T
D000968:Antimycin A / Q000494:pharmacology; D018929:Cell Culture Techniques / Q000379:methods*; D019069:Cell Respiration / Q000187:drug effects / Q000502:physiology*; D004563:Electrochemistry / Q000379:methods*; D000431:Ethanol / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D008839:Microelectrodes; D018613:Microscopy, Confocal / Q000379:methods*; D010100:Oxygen / Q000378:metabolism*; D011190:Potassium Cyanide / Q000494:pharmacology; D013050:Spectrometry, Fluorescence / Q000379:methods*
D000431:Ethanol; D000968:Antimycin A; D011190:Potassium Cyanide; D010100:Oxygen