Fluorescent labelling of ribonucleosides at 2'-terminus; comparative fluorescence studies.
In case of RNA's, multiple labelling can be achieved by exploiting the available 2'-OH position of sugar moiety of nucleosides. N-protected nucleoside viz. cytidine has been prepared using a selective photolabile group i.e. 2-nitrobenzyloxycarbonyl. After protection of 5',3'-OH with 1,1,3,3,-tetraisopropyl disiloxyl group, 2'-OH was selectively activated by using N,N'-carbonyl diimidazole (CDI) and subsequently condensed with dansyl amide. After usual deprotection step comparative fluorescence studies of the monomer were carried out using different solvents/buffers.
Misra
A
A
Nucleic Acids Research Laboratory, Department of Chemistry, University of Allahabad, Allahabad-211 002, India.
Tripathi
S
S
Misra
K
K
D001665:Binding Sites; D002021:Buffers; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry*; D015394:Molecular Structure; D010777:Photochemistry; D012263:Ribonucleosides / Q000138:chemical synthesis* / Q000737:chemistry*; D012997:Solvents; D013050:Spectrometry, Fluorescence
D002021:Buffers; D005456:Fluorescent Dyes; D012263:Ribonucleosides; D012997:Solvents