title: Beta-amyloid peptides in cerebrospinal fluid of patients with Creutzfeldt-Jakob disease.
abstract: Decreased levels of beta-amyloid peptide 1-42 (Abeta1-42) in cerebrospinal fluid (CSF) are a characteristic feature of Alzheimer's disease (AD) but recently were also observed in Creutzfeldt-Jakob disease (CJD). We analyzed the CSF of patients with CJD, and AD and nondemented controls using a quantitative urea-based Abeta sodium dodecyl sulfate polyacrylamide gel electrophoresis immunoblot. Like in AD and nondemented controls, we found a highly conserved pattern of carboxyterminally truncated Abeta1-37/38/39 in addition to Abeta1-40/42 also in CJD patients. By the introduction of the ratio Abeta1-39 to Abeta1-42, CJD and AD can effectively be differentiated. We conclude that the immunoblot shows disease-specific CSF Abeta peptide patterns in CJD and AD and suppose that measurement of the Abeta peptide pattern seems to be a promising diagnostic tool in the differential diagnosis of dementias.
authors.affiliation: Department of Psychiatry, University Erlangen-Nürnberg, Germany.
authors.forename: Jens
authors.initials: J
authors.lastname: Wiltfang
authors.forename: Hermann
authors.initials: H
authors.lastname: Esselmann
authors.forename: Alexander
authors.initials: A
authors.lastname: Smirnov
authors.forename: Mirko
authors.initials: M
authors.lastname: Bibl
authors.forename: Lukas
authors.initials: L
authors.lastname: Cepek
authors.forename: Petra
authors.initials: P
authors.lastname: Steinacker
authors.forename: Brit
authors.initials: B
authors.lastname: Mollenhauer
authors.forename: Katharina
authors.initials: K
authors.lastname: Buerger
authors.forename: Harald
authors.initials: H
authors.lastname: Hampel
authors.forename: Sabine
authors.initials: S
authors.lastname: Paul
authors.forename: Manuela
authors.initials: M
authors.lastname: Neumann
authors.forename: Manuel
authors.initials: M
authors.lastname: Maler
authors.forename: Inga
authors.initials: I
authors.lastname: Zerr
authors.forename: Johannes
authors.initials: J
authors.lastname: Kornhuber
authors.forename: Hans A
authors.initials: HA
authors.lastname: Kretzschmar
authors.forename: Sigrid
authors.initials: S
authors.lastname: Poser
authors.forename: Markus
authors.initials: M
authors.lastname: Otto
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000134:cerebrospinal fluid; D016229:Amyloid beta-Peptides / Q000134:cerebrospinal fluid*; D001057:Apolipoproteins E / Q000134:cerebrospinal fluid; D007562:Creutzfeldt-Jakob Syndrome / Q000134:cerebrospinal fluid*; D004591:Electrophoresis, Polyacrylamide Gel; D004660:Encephalitis / Q000134:cerebrospinal fluid; D005260:Female; D006801:Humans; D015151:Immunoblotting; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000134:cerebrospinal fluid; D016875:tau Proteins / Q000134:cerebrospinal fluid
chemical_list: D016229:Amyloid beta-Peptides; D001057:Apolipoproteins E; D010446:Peptide Fragments; D016875:tau Proteins