title: [On preparation the monoclonal antibody against synthetic antigen of cyclosporine A].
abstract: OBJECTIVE
  To study the methods for preparing the monoclonal antibody against synthetic antigen of cyclosporine A.
  
  
  METHODS
  These included using photochemical reaction to couple cyclosporine A with poly-L-lysine as synthetic antigen, immunizing BALB/c mice, fusing spleen cells and SP2/0 cells. Then hybridoma cells were repeatedly screened and subcloned. The monoclonal antibodies were identified by indirect ELISA.
  
  
  RESULTS
  Four hybridoma cells in secreting anti-cyclosporine A monoclonal antibodies were obtained. Only two showed high titer and some specificity.
  
  
  CONCLUSIONS
  The methods of antigen synthesis and immunization were practicable. But the methods of antibody preparation and preservation need to be improved.
authors.affiliation: Department of Infectious Disease, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Mina
authors.initials: M
authors.lastname: Chen
authors.forename: Xinjian
authors.initials: X
authors.lastname: Fan
authors.forename: Dapeng
authors.initials: D
authors.lastname: Wei
authors.forename: Qiongying
authors.initials: Q
authors.lastname: Wu
authors.forename: Ping
authors.initials: P
authors.lastname: Feng
authors.forename: Xiaoju
authors.initials: X
authors.lastname: Lü
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D016572:Cyclosporine / Q000276:immunology*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D011107:Polylysine / Q000276:immunology*; D013154:Spleen / Q000166:cytology
chemical_list: D000911:Antibodies, Monoclonal; D011107:Polylysine; D016572:Cyclosporine