[Expression of Fas, FasL, and NF-kappa B in the process of osteoclast-like cell apoptosis effected by sodium fluoride].
OBJECTIVE
To detect the changes in the expression of apoptosis signals: Fas, FasL and NF-kappa B in the process of osteoclast-like cell (OLC) apoptosis effected by sodium fluoride.


METHODS
After co-culture of osteoclast-like cells with 0, 5, 10 and 15 mg/L sodium fluoride, Fas, FasL and NF-kappa B antibody expressions were detected by immune-histochemistry.


RESULTS
The expression of Fas and FasL increased with the concentration of the sodium fluoride, however the expression of NF-kappa B decreased with the concentration of sodium fluoride.


CONCLUSIONS
In the process of OLC apoptosis induced by sodium fluoride, the expression of Fas and FasL increased, and that of NF-kappa B decreased with the concentration of sodium fluoride respectively, and the changes of the expression present a dose-dependent pattern.
Sun
Yuan-ming
YM
Department of Radiobiology, Institute of Radiation Medicine, CAMS, PUMC, Tianjin 300192, China.
Yang
Fu-jun
FJ
Li
Yu-min
YM
Lu
Biao
B
Zhu
Mei
M
Qiu
Ming-cai
MC
D000818:Animals; D017209:Apoptosis; D053222:Fas Ligand Protein; D005260:Female; D005459:Fluorides / Q000494:pharmacology*; D008024:Ligands; D008562:Membrane Glycoproteins / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000378:metabolism*; D010010:Osteoclasts / Q000166:cytology* / Q000378:metabolism; D019014:fas Receptor / Q000378:metabolism*
D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; D008024:Ligands; D008562:Membrane Glycoproteins; D016328:NF-kappa B; D019014:fas Receptor; D005459:Fluorides