title: Biolistic transfection.
abstract: Recent improvements in the biolistic technique and devices have increased its usefulness for transfection of neurons. With these recent advances, both dissociated and slice cultures can be transfected at reasonably high rates. This chapter focuses on the parameters that determine the successful biolistic transfection of neurons in both types of cultures.
authors.affiliation: Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
authors.forename: Paul C
authors.initials: PC
authors.lastname: Bridgman
authors.forename: Michael E
authors.initials: ME
authors.lastname: Brown
authors.forename: Irina
authors.initials: I
authors.lastname: Balan
mesh_terms: D000818:Animals; D019470:Biolistics / Q000295:instrumentation / Q000379:methods*; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002478:Cells, Cultured / Q000166:cytology / Q000502:physiology*; D004247:DNA / Q000235:genetics / Q000494:pharmacology; D006801:Humans; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D010316:Particle Size; D014162:Transfection / Q000295:instrumentation / Q000379:methods*
chemical_list: D004247:DNA