Modulation of MUC1 and blood group antigen expression in gastric adenocarcinoma cells by cytokines.
Immunohistological studies demonstrated that MUC1 expression in gastric cancer is associated with a poor prognosis. As a mediator of cell-cell interactions, MUC1 may also be involved in metastasis. However, these aspects are of relevance since cytokine levels are locally increased as a consequence of peritumorous inflammatory response and coexisting chronic gastritis. Therefore we analyzed the potential influence of several cytokines on the expression of tumor-associated MUC1 and Lewis blood group antigens in gastric carcinoma cells. Gastric cancer cell lines AGS and KATOIII were incubated with the cytokines interleukin-1beta, interferon-gamma, tumor necrosis factor-alpha (TNF-alpha), and hepatocyte growth factor over a period of 72 h. Expressions of mucin antigens and cytokine secretion were measured by immunocytochemistry and/or enzyme-linked immunosorbent assay (ELISA). Analysis by fluorescence-activated cell sorter (FACS) demonstrated that MUC1 and sialyl Lewis A reactivities of AGS cells were increased significantly following TNF-alpha stimulation but not by other cytokines. Expression of mucin-associated antigens by cell line KATOIII was not affected by any of the employed cytokines. These data provide evidence that TNF-alpha can raise the expression of important mucin peptide as well as mucin-associated carbohydrate antigens and thereby potentially influence the progression of gastric carcinomas.
Grohmann
Georg P M
GP
Institute of Pathology, University of Cologne, Joseph-Stelzmann-Street 9, D-50931 Cologne, Germany.
Schirmacher
Peter
P
Manzke
Oliver
O
Hanisch
Franz Georg
FG
Dienes
Hans P
HP
Baldus
Stephan E
SE
D000230:Adenocarcinoma / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D016207:Cytokines / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D007983:Lewis Blood Group Antigens / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D018396:Mucin-1 / Q000276:immunology / Q000378:metabolism*; D011817:Rabbits; D018121:Receptors, Cytokine / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D013274:Stomach Neoplasms / Q000276:immunology / Q000378:metabolism*
D016207:Cytokines; D007983:Lewis Blood Group Antigens; D018396:Mucin-1; D018121:Receptors, Cytokine