title: Use of a 13C-aminopyrine blood test: first clinical impressions.
abstract: The purpose of this study was to collect initial data to determine the potential clinical usefulness of a 13C-aminopyrine demethylation blood test, and whether additional clinical investigation is warranted. Six dogs, initially suspected of having hepatic disease based on their history, physical examination, imaging studies, general laboratory parameters, or any combination of the above, were enrolled in the study. A baseline blood sample was collected, 2 mg/kg 13C-aminopyrine was administered intravenously, and another blood sample was collected 45 min afterwards. Carbon dioxide was extracted from the blood samples and analyzed using fractional mass spectrometry. Results from the 13C-aminopyrine demethylation blood test were compared to clinical data and histologic findings. Intravenous administration of 13C-aminopyrine leads to a decrease in the percent dose of 13C recovered from dogs with histologically confirmed liver disease. Based on our results, a full-scale investigation of the potential clinical usefulness of a 13C-aminopyrine demethylation blood test for assessment of hepatic function in dogs is warranted.
authors.affiliation: The Bobst Hospital of The Animal Medical Center, 510 East 62nd Street, New York, New York 10021, USA. Deirdre.Chiaramonte@amcny.org
authors.forename: Deirdre
authors.initials: D
authors.lastname: Chiaramonte
authors.forename: Jörg M
authors.initials: JM
authors.lastname: Steiner
authors.forename: John D
authors.initials: JD
authors.lastname: Broussard
authors.forename: Keith
authors.initials: K
authors.lastname: Baer
authors.forename: Sheila
authors.initials: S
authors.lastname: Gumminger
authors.forename: Erik M
authors.initials: EM
authors.lastname: Moeller
authors.forename: David A
authors.initials: DA
authors.lastname: Williams
authors.forename: Renée
authors.initials: R
authors.lastname: Shumway
mesh_terms: D000632:Aminopyrine / Q000008:administration & dosage / Q000097:blood*; D000818:Animals; D002247:Carbon Isotopes; D004283:Dog Diseases / Q000097:blood / Q000175:diagnosis*; D004285:Dogs; D005260:Female; D007275:Injections, Intravenous / Q000662:veterinary; D008099:Liver / Q000378:metabolism; D008107:Liver Diseases / Q000175:diagnosis / Q000662:veterinary*; D008111:Liver Function Tests / Q000379:methods / Q000662:veterinary; D008297:Male; D013058:Mass Spectrometry / Q000662:veterinary
chemical_list: D002247:Carbon Isotopes; D000632:Aminopyrine