title: The breast cancer-associated gene Di12 has oncogenic activity.
abstract: The breast cancer-associated gene Di12 encodes a novel protein, which was found overexpressed in invasive ductal carcinomas of the breast. In experiments designed to assess the role of the Di12 gene in oncogenesis, the overexpression of 339 N-terminal amino acids of this gene in NIH3T3 cells resulted in cellular transformation and in vivo tumorigenesis. NIH3T3-Di12 tumor cell growth was partly reversible upon Di12 antisense treatment. In addition, transformation of the ER+ human breast cancer cell line MCF-7 resulted in hormone independent growth of these tumors in nude mice. Di12 expression in NIH3T3 and MCF-7 tumor cells was confirmed by RT-PCR and mabDi12 immunostaining. Immunohistochemistry using mabDi12 on an arrayed collection of 106 invasive breast tumors further underlined the expression of the gene in over 75% of advanced stage breast cancers. Our data indicate that Di12 expression is oncogenic in in vitro transformation and in vivo tumorigenic assays.
authors.affiliation: Molecular and Cell Biology Research, Laboratory of Molecular Pathology, Sunnybrook and Women's College Health Science Centre, University of Toronto, Toronto, Canada.
authors.forename: Angelika M
authors.initials: AM
authors.lastname: Burger
authors.forename: Renate
authors.initials: R
authors.lastname: Grugel
authors.forename: Göran
authors.initials: G
authors.lastname: Landberg
authors.forename: Richard
authors.initials: R
authors.lastname: Kitching
authors.forename: Arun
authors.initials: A
authors.lastname: Seth
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D005260:Female; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009363:Neoplasm Proteins; D009857:Oncogenes / Q000235:genetics; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D014163:Transfer, Psychology; D014407:Tumor Cells, Cultured
chemical_list: C110511:Di12 protein, human; D009363:Neoplasm Proteins; D011506:Proteins