title: Characterisation of CadR from Pseudomonas aeruginosa: a Cd(II)-responsive MerR homologue.
abstract: cadR from Pseudomonas aeruginosa encodes a transcriptional regulatory protein which responds to Cd(II)>>Zn(II)>Hg(II) at its cognate promoter PcadA. CadR will also act to induce transcription at the Escherichia coli ZntR cognate promoter, PzntA, however, the induction profile is altered to Hg(II)>Cd(II)>Zn(II). Two separate single base pair deletions within PzntA result in further alteration of relative specificity in metal-ion induction profile for CadR. This demonstrates that the operator/promoter sequence can play a role in defining optimal ligand response and that for these regulators specificity is not solely a function of the regulatory protein.
authors.affiliation: Syngenta, Jealott's Hill International Research Centre, Bracknell, Berkshire RG42 6EY, UK.
authors.forename: K R
authors.initials: KR
authors.lastname: Brocklehurst
authors.forename: S J
authors.initials: SJ
authors.lastname: Megit
authors.forename: A P
authors.initials: AP
authors.lastname: Morby
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D002104:Cadmium / Q000494:pharmacology; D004269:DNA, Bacterial / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; C432939:CadR protein, Pseudomonas; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; C051124:MerR protein, Bacteria; D014157:Transcription Factors; D002104:Cadmium