title: A new mitochondrial fluorescent zinc sensor.
abstract: A novel cationic fluorescent zinc (Zn2+) indicator (RhodZin-3) with nanomolar affinity for Zn2+ has been synthesized. RhodZin-3 exhibits large pH-independent fluorescence increases in the orange region of the visible wavelength spectrum with increasing zinc concentrations, and no sensitivity to physiologically relevant Ca2+ concentrations. Experiments in neuronal cell cultures show that RhodZin-3 effectively localizes into mitochondria and detects changes of intramitochondrial free Zn2+ ([Zn2+]m).
authors.affiliation: Department of Neurology, University of California, Irvine, CA 92697-4292, USA.
authors.forename: Stefano L
authors.initials: SL
authors.lastname: Sensi
authors.forename: Dien
authors.initials: D
authors.lastname: Ton-That
authors.forename: John H
authors.initials: JH
authors.lastname: Weiss
authors.forename: Anca
authors.initials: A
authors.lastname: Rothe
authors.forename: Kyle R
authors.initials: KR
authors.lastname: Gee
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D001923:Brain Chemistry; D002478:Cells, Cultured; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D051379:Mice; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000737:chemistry*; D016548:Prosencephalon / Q000166:cytology; D012680:Sensitivity and Specificity; D014966:Xanthenes / Q000138:chemical synthesis / Q000737:chemistry*; D015032:Zinc / Q000032:analysis* / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; C484817:RhodZin-3; D014966:Xanthenes; D015032:Zinc