Development of HIV-1 dependent gene expression vector with the Cre/loxP (ON) system.
We used the HIV-1 5'LTR and the Cre/loxP system to develop an anti-gene expression system. The LTR promoter of HIV-1 has a specific activity that includes the intermediary region of gag, as shown in a previous report. We constructed the U5-region of HIV-1 as the target of the ribozyme expression vector (pCre/loxP-Rz), with the Cre/loxP system under the control of this LTR promoter. The function of this vector is to induce the HIV-1 dependent ribozyme-mediated inhibition in a dose-responsive manner. Furthermore, ribozyme mRNA expression was detected in the presence of pNL4-3 in HeLa-CD4+ cells. These studies are expected to yield novel applications of antiviral strategies for HIV-1.
Nagawa
Takashi
T
Department of Industrial Chemistry, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, Japan.
Habu
Yuichiro
Y
Matsumoto
Norihiko
N
Takeuchi
Hiroaki
H
Kurosaki
Naoko
N
Takaku
Hiroshi
H
D015870:Gene Expression; D005822:Genetic Vectors; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000235:genetics*; D019426:Integrases / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics
D014764:Viral Proteins; C045073:Cre recombinase; D019426:Integrases