The Saccharomyces cerevisiae chitinase, encoded by the CTS1-2 gene, confers antifungal activity against Botrytis cinerea to transgenic tobacco.
The Saccharomyces cerevisiae chitinase, encoded by the CTS1-2 gene has recently been confirmed by in vitro tests to possess antifungal abilities. In this study, the CTS1-2 gene has been evaluated for its in planta antifungal activity by constitutive overexpression in tobacco plants to assess its potential to increase the plant's defence against fungal pathogens. Transgenic tobacco plants, generated by Agrobacterium-mediated transformation, showed stable integration and inheritance of the transgene. Northern blot analyses conducted on the transgenic tobacco plants confirmed transgene expression. Leaf extracts from the transgenic lines inhibited Botrytis cinerea spore germination and hyphal growth by up to 70% in a quantitative in vitro assay, leading to severe physical damage on the hyphae. Several of the F1 progeny lines were challenged with the fungal pathogen, B. cinerea, in a detached leaf infection assay, showing a decrease in susceptibility ranging from 50 to 70%. The plant lines that showed increased disease tolerance were also shown to have higher chitinase activities.
Carstens
M
M
Department of Viticulture and Oenology, Institute for Wine Biotechnology, Stellenbosch University, Stellenbosch 7602, South Africa.
Vivier
M A
MA
Pretorius
I S
IS
D001483:Base Sequence; D015152:Blotting, Northern; D020171:Botrytis / Q000472:pathogenicity*; D002688:Chitinases / Q000235:genetics* / Q000378:metabolism; D017931:DNA Primers; D030821:Plants, Genetically Modified / Q000235:genetics / Q000382:microbiology*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics / Q000382:microbiology*; D014170:Transformation, Genetic
D017931:DNA Primers; D002688:Chitinases