Search for Lawsonia intracellularis and Bilophila wadsworthia in malabsorption-diseased chickens.
Proliferative enteropathy is an important enteric disease caused by Lawsonia intracellularis. A wide range of host species can be infected by the same bacterium, yet the clinico-pathologic features among these hosts remains almost identical. The disease has been recognized regularly among ratites, but not in other avian families, such as galliforms, even though these suffer uncharacterized enteric conditions. Fresh ileum-colon contents were obtained from 228, 3- to 8-week-old chickens with enteric disease, kept at 14 large commercial farms in the southern USA. DNA was extracted from each sample and subjected to polymerase chain reactions (PCR) with primers specific to eubacterial DNA, L. intracellularis, and Bilophila wadsworthia. All chicken samples were positive for eubacterial DNA, 29 chickens (13%) were positive for B. wadsworthia DNA, and none were positive for L. intracellularis DNA. Given the ubiquitous nature of L. intracellularis, we consider it likely that some avian families do not carry the necessary mechanism for L. intracellularis viability. Bilophila wadsworthia appears to be a consistent member of the colonic flora of some host animals. Neither bacterium appears to be associated with malabsorption syndromes in chickens.
McOrist
Steven
S
Department of Biomedical Sciences, Tufts University School of Veterinary Medicine, 200 Westboro Road, North Grafton, Massachusetts 01536, USA. steven.mcorist@tufts.edu
Keller
Linda
L
McOrist
Alexandra L
AL
D000818:Animals; D002645:Chickens; D004269:DNA, Bacterial / Q000032:analysis; D020564:Deltaproteobacteria / Q000235:genetics / Q000302:isolation & purification*; D045824:Desulfovibrionaceae Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary; D016905:Gram-Negative Bacterial Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D020639:Lawsonia Bacteria / Q000235:genetics / Q000302:isolation & purification*; D008286:Malabsorption Syndromes / Q000209:etiology / Q000382:microbiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011201:Poultry Diseases / Q000453:epidemiology / Q000382:microbiology*
D004269:DNA, Bacterial