title: Studies on skin aging. Preparation and properties of fucose-rich oligo- and polysaccharides. Effect on fibroblast proliferation and survival.
abstract: Skin aging represents an important chapter of connective tissue aging and concerns an organ of vital importance. Here we describe the preparation as well as the biological properties of fucose-rich oligo- and polysaccharides (FROPs), composed of polymers of a trisaccharide containing galactose, acetyl galacturonic acid and fucose, from the original high molecular weight bacterial polysaccharide (Fucogel), Solabia, France). Using endoglycosidases, oligo- and polysaccharides were prepared and characterized by physical and chemical procedures. The non-reducing end-groups comprise equal amounts of galactose and fucose. The here-described biological properties are: stimulation of cell proliferation of cultured human skin fibroblasts, protection of cells against ascorbate-induced cytotoxicity due to the release of reactive oxygen species (ROS). Properties elsewhere described concern the inhibition of matrix metallo-proteinases 2 and 9 (MMP-2 and MMP-9), their expression and activation. Using fluorescein isothiocyanate (FITC)-labeled polysaccharides, their interaction with cell membranes and also their penetration and accumulation in cells, especially in the cell nucleus could be demonstrated, probably via cell-membrane receptor-mediated mechanisms. We describe some of the symptoms of skin aging and show, that the here-described polysaccharide preparations are susceptible to slow down some of the cellular and molecular mechanisms involved, partly by the mediation of the above-mentioned receptors, partly by acting directly on the regulation of gene expression.
authors.affiliation: Faculté de Médecine Broussais-Hôtel Dieu, Laboratoire Universitaire de Recherche en Ophtalmologie, Université Pierre et Marie Curie (Paris 6), 1, place du Parvis-Notre-Dame, 75181 Paris cedex 4, France.
authors.forename: G
authors.initials: G
authors.lastname: Péterszegi
authors.forename: N
authors.initials: N
authors.lastname: Isnard
authors.forename: A M
authors.initials: AM
authors.lastname: Robert
authors.forename: L
authors.initials: L
authors.lastname: Robert
mesh_terms: D001205:Ascorbic Acid / Q000633:toxicity; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000187:drug effects*; D005643:Fucose / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D009844:Oligosaccharides / Q000737:chemistry / Q000494:pharmacology*; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000494:pharmacology*; D012867:Skin / Q000166:cytology / Q000187:drug effects; D015595:Skin Aging / Q000187:drug effects*
chemical_list: D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; D005643:Fucose; D001205:Ascorbic Acid