Development of a sensitive gene expression reporter system and an inducible promoter-repressor system for Clostridium acetobutylicum.
A sensitive gene expression reporter system was developed for Clostridium acetobutylicum ATCC 824 by using a customized gusA expression cassette. In discontinuous cultures, time course profiles of beta-glucuronidase specific activity reflected adequately in vivo dynamic up- and down-regulation of acidogenesis- and/or solventogenesis-associated promoter expression in C. acetobutylicum. Furthermore, a new inducible gene expression system was developed in C. acetobutylicum, based on the Staphylococcus xylosus xylose operon promoter-repressor regulatory system.
Girbal
Laurence
L
Centre de Bioingénierie Gilbert Durand, Laboratoire de Biotechnologie-Bioprocédés, UMR CNRS 5504, UMR INRA 792, INSA, France. girbal@insa-tlse.fr
Mortier-Barrière
Isabelle
I
Raynaud
Frédéric
F
Rouanet
Céline
C
Croux
Christian
C
Soucaille
Philippe
P
D001483:Base Sequence; D003013:Clostridium / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D006864:Hydrogenase / Q000235:genetics; D007506:Iron-Sulfur Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000502:physiology*; D014158:Transcription, Genetic
D007506:Iron-Sulfur Proteins; D012097:Repressor Proteins; C118830:iron hydrogenase; D006864:Hydrogenase; D005966:Glucuronidase