[Production and application of rabbit anti-imitative spider dragline silk protein polyclonal antibody].
A synthetic spider dragline gene s600 was cloned into fusion protein expression vector pGEX-KG and expressed in Escherichia coli. Protein S600 was purified and rabbit antiserum was prepared. Amino acid composition analysis confirmed the right expression of S600. Western blot analysis revealed that anti-S600 antiserum could react with natural spider silk, so the synthetic dragline protein, designed by the authors, shares similar immunological characteristics with the natural spider silk. An ELISA system was also established for the quantitative detection of synthetic dragline protein expression in silk gland (or in the cocoon) of transgenic silkworm.
Guo
Ting-Qing
TQ
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, China.
Zhao
Yun
Y
Wang
Sheng-Peng
SP
Dong
Liang
L
Lu
Chang-De
CD
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D001483:Base Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics; D005349:Fibroins; D007106:Immune Sera / Q000276:immunology*; D008969:Molecular Sequence Data; D011506:Proteins / Q000032:analysis / Q000276:immunology*; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification; D013112:Spiders / Q000737:chemistry*
D000906:Antibodies; D007106:Immune Sera; D011506:Proteins; D011993:Recombinant Fusion Proteins; C076815:spidroin 2; D005349:Fibroins