Mutations induced by glyoxal and methylglyoxal in mammalian cells.
To investigate the mutation spectra of glyoxal and methylglyoxal in mammalian cells, we analyzed mutations in a bacterial suppressor tRNA (supF) gene in the shuttle vector plasmid pMY189. The cytotoxicity and the mutation frequency increased according to the doses of glyoxal and methylglyoxal. The majority of glyoxal-induced mutations (65%) were base-pair substitutions, in which G:C-->C:G transversions were predominant. In the mutants induced by methylglyoxal, multi-base deletions were predominant (50%), followed by base-pair substitutions (35%), in which G:C-->C:G and G:C-->T:A transversions were predominant.
Murata-Kamiya
N
N
Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan.
Kamiya
H
H
Kaji
H
H
Kasai
H
H
D000818:Animals; D020029:Base Pairing; D019556:COS Cells; D004252:DNA Mutational Analysis; D006037:Glyoxal / Q000633:toxicity*; D009153:Mutagens / Q000633:toxicity; D009154:Mutation; D010957:Plasmids / Q000187:drug effects / Q000235:genetics; D017354:Point Mutation; D011765:Pyruvaldehyde / Q000633:toxicity*; D017384:Sequence Deletion
D009153:Mutagens; D006037:Glyoxal; D011765:Pyruvaldehyde