title: Comparison of the growth-inhibitory effect of Hypericum perforatum L. extracts, differing in the concentration of phloroglucinols and flavonoids, on leukaemia cells.
abstract: In this study we compared, simultaneously, the growth-inhibitory effect of Hypericum perforatum L. extracts, containing various amounts of hyperforin (A 3.25%; B 2.21%; C 0.21% w/w) and flavonoids (A and B 5.3%; C 10% w/w), but closely same amounts of naphthodiantrones (0.3%) on two leukaemic cell lines K562 and U937 in the WST-1 assay. The GI50 (concentration of extracts which caused 50% of cell growth inhibition) for H. perforatum extracts analysed and characterized by HPLC for their biologically active constituents was 248.3-621.3 microg mL(-1) in K562 and 378.2-911.7 microg mL(-1) in U937 cells. The corresponding values of the three main groups were 1.6-3.9 microM naphthodianthrones, 1.0-40.7 microM phloroglucinols and 30.5-68.5 microM flavonoids. The results of this study supported the hypothesis that, apart from hyperforin and flavonoids, other components of the extract could be involved in its growth-inhibitory effect that it exerts without light activation.
authors.affiliation: Department of Internal Medicine F NUK 104, University Hospital Zürich, Rämistrasse 100, 8091 Zürich, Switzerland. katarina.hostanska@access.unizh.ch
authors.forename: Katarina
authors.initials: K
authors.lastname: Hostanska
authors.forename: Silvia
authors.initials: S
authors.lastname: Bommer
authors.forename: Michel
authors.initials: M
authors.lastname: Weber
authors.forename: Blerim
authors.initials: B
authors.lastname: Krasniqi
authors.forename: Reinhard
authors.initials: R
authors.lastname: Saller
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis / Q000494:pharmacology*; D001643:Bridged Bicyclo Compounds; D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology*; D006801:Humans; D020902:Hypericum / Q000737:chemistry; D020128:Inhibitory Concentration 50; D020014:K562 Cells; D008027:Light; D010696:Phloroglucinol / Q000032:analysis / Q000494:pharmacology* / Q000031:analogs & derivatives; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology; D013729:Terpenes / Q000494:pharmacology; D020298:U937 Cells
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D001643:Bridged Bicyclo Compounds; D005419:Flavonoids; D010936:Plant Extracts; D013729:Terpenes; D010696:Phloroglucinol; C001654:hyperforin