[Culture and identification of dendritic cells from the peripheral blood of patients with laryngeal squamous cell carcinoma in vitro].
OBJECTIVE
To culture dendritic cells (DC) from peripheral blood of patients with laryngeal carcinoma for therapeutic aid.


METHODS
Adherent peripheral blood mononuclear cells from peripheral blood were cultured with 15 ng/ml rhGM-CSF and 7 ng/ml rhIL-4 for one or two weeks. The purity of DC was detected by immunocytochemistry method. The mixed leukocyte reactions stimulated by DC loaded with laryngeal carcinoma antigen were tested by measuring 3H-TdR uptake.


RESULTS
A considerable number of suspended cells with spicular or dendritic appearance were observed after 1 week of culture, and their mitochondria were rich in cytoplasm. The positivity of DC was about 30%-60%. DC loaded with laryngeal antigen could induce proliferation of syngeneic T lymphocytes.


CONCLUSIONS
A large number of DC with high purity can be cultured from peripheral blood of patients with laryngeal carcinoma in vitro. It may be used in further experimental studies for clinical applications.
Feng
Yun
Y
Department of Immunology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China.
Tan
Hui-bing
HB
Su
Hua
H
Zhang
Bao-quan
BQ
Li
Ruo-fan
RF
He
Wei
W
D002294:Carcinoma, Squamous Cell / Q000097:blood*; D002469:Cell Separation; D002478:Cells, Cultured; D003470:Culture Media; D003713:Dendritic Cells / Q000473:pathology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D015847:Interleukin-4 / Q000494:pharmacology; D007822:Laryngeal Neoplasms / Q000097:blood*; D011994:Recombinant Proteins / Q000494:pharmacology
D003470:Culture Media; D011994:Recombinant Proteins; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor