Presence of Vicia graminea or Vicia unijuga lectin-binding (Vgu) glycoproteins with and without Thomsen-Friedenreich (T) antigen in normal human seminal plasma.
Vgu glycoprotein (Vicia graminea lectin- or Vicia unijuga lectin-binding glycoprotein) has been reported as oncofetal antigen, which is found in many kind of tumor tissues, amniotic fluid and fetal membranes. In autoradiography with an 125I-labeled Vicia unijuga lectin (VUA) probe and an 125I-labeled Arachis hypogaea lectin (PNA, anti-T lectin) probe, seminal plasma samples of eight healthy men gave 2-7 Vgu glycoproteins without T antigen, 1-2 Vgu glycoproteins with Thomsen-Friedenreich antigen (T antigen) and 1-8 T-antigen glycoproteins, respectively. These results show that in addition to T-antigen glycoproteins, normal human seminal plasma contains Vgu glycoproteins and Vgu glycoproteins with T antigen as seminal plasma components as well as other tumor markers such as CA19-9, CA-125 and CEA.
Yamaguchi
Y
Y
Department of Biochemistry, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya, Hyogo 663-8501, Japan. yyama@hyo-med.ac.jp
Otsuka
K
K
Habu
Y
Y
Ohyama
K
K
Kurotsu
T
T
Ookawara
T
T
Ohkuma
S
S
Yamakawa
T
T
Suzuki
K
K
D015295:Antigens, Tumor-Associated, Carbohydrate / Q000302:isolation & purification*; D001345:Autoradiography; D014408:Biomarkers, Tumor / Q000302:isolation & purification*; D006023:Glycoproteins / Q000302:isolation & purification*; D006360:Heat-Shock Proteins; D006801:Humans; D008297:Male; D012260:Ribonucleases; D012661:Semen / Q000378:metabolism*
D015295:Antigens, Tumor-Associated, Carbohydrate; D014408:Biomarkers, Tumor; D006023:Glycoproteins; D006360:Heat-Shock Proteins; C016467:Thomsen-Friedenreich antigen; D012260:Ribonucleases; C490788:RIDA protein, human