Expression of recombinant human ICOS and in vitro characterization of its bioactivity on B lymphocytes.
Inducible costimulator (ICOS) is a novel costimulatory molecule expressed in activated T cell and has critical regulation effect on special immune response. In this study, the cDNA encoding human ICOS was cloned from activated tonsil cells via RT-PCR, and was expressed in E. coli on pET28 expression vector. The recombinant ICOS protein expressed from E. coli showed a molecular weight of 14 kD on SDS-polyacrylamide gel electrophoresis and was further confirmed by Western blot. In presence of IL-10, the purified rhICOS significantly increased in vitro B cell growth stimulated by pokeweed mitogen (PWM), and enhanced the secretion of IgG from B cells.
Deng
Zhong-Bin
ZB
Biotechnology Research Institute, Soochow University, Suzhou 215007, China. smbxuegz@public1.sz.js.cn
Lu
Chang-Ming
CM
Huang
Wei-Da
WD
Shen
Li-Qin
LQ
Zhu
Wei
W
Ma
Hong-Bing
HB
Fan
Pan-Sheng
PS
Zhang
Xue-Guang
XG
D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D001402:B-Lymphocytes / Q000187:drug effects*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D060889:Inducible T-Cell Co-Stimulator Protein; D008213:Lymphocyte Activation / Q000187:drug effects; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology
D000945:Antigens, Differentiation, T-Lymphocyte; C556692:ICOS protein, human; D007074:Immunoglobulin G; D060889:Inducible T-Cell Co-Stimulator Protein; D011994:Recombinant Proteins