Single long DNA molecule analysis using fluorescence microscopy.
Single long DNA molecule (T4 DNA) in agarose gel was visualized with a fluorescence microscope. We confirmed alternating current electric fields is effective for stretching of single DNA molecule in agarose gel. This stretching phenomenon was observed with wide range of agarose gel concentration from 0.5%(W/V) to 1.5%. From this observation, the presence of agarose gel fiber is essential for this stretching phenomenon. The stretching process of several DNA molecules in gel shows discontinuity, which is never observed in polymer systems. It would be based on topological restriction from gel fibers.
Kaji
N
N
Department of Medical Chemistry, Faculty of Pharmaceutical Sciences, University of Tokushima, Tokushima 770-8505, Japan.
Ueda
M
M
Baba
Y
Y
D017122:Bacteriophage T4 / Q000737:chemistry; D001583:Benzoxazoles; D004279:DNA, Viral / Q000737:chemistry* / Q000302:isolation & purification / Q000648:ultrastructure*; D004587:Electrophoresis, Agar Gel; D005456:Fluorescent Dyes; D007364:Intercalating Agents; D008856:Microscopy, Fluorescence / Q000379:methods*; D011806:Quinolinium Compounds
D001583:Benzoxazoles; D004279:DNA, Viral; D005456:Fluorescent Dyes; D007364:Intercalating Agents; D011806:Quinolinium Compounds; C075296:1,1'-((4,4,7,7-tetramethyl)-4,7-diazaundecamethylene)bis-4-(3-methyl-2,3-dihydro(benzo-1,3-oxazole)-2-methylidene)quinolinium