title: SH2-containing inositol 5-phosphatases 1 and 2 in blood platelets: their interactions and roles in the control of phosphatidylinositol 3,4,5-trisphosphate levels.
abstract: Src homology domain 2-containing inositol 5-phosphatases 1 and 2 (SHIP1 and SHIP2) are capable of dephosphorylating the second messenger PtdIns(3,4,5) P3 (phosphatidylinositol 3,4,5-trisphosphate) and interacting with several signalling proteins. SHIP1 is essentially expressed in haematopoietic cells, whereas SHIP2, a closely related enzyme, is ubiquitous. In the present study, we show that SHIP1 and SHIP2 are expressed as functional PtdIns(3,4,5) P3 5-phosphatases in human blood platelets and are capable of interacting when these two lipid phosphatases are co-expressed, either naturally (platelets and A20 B lymphoma cells) or artificially (COS-7 cells). Using COS-7 cells transfected with deletion mutants of SHIP2, we demonstrate that the Src homology domain 2 of SHIP2 is the minimal and sufficient protein motif responsible for the interaction between the two phosphatases. These results prompted us to investigate the relative importance of SHIP1 and SHIP2 in the control of PtdIns(3,4,5) P3 levels in platelets using homozygous or heterozygous SHIP1- or SHIP2-deficient mice. Our results strongly suggest that SHIP1, rather than SHIP2, plays a major role in controlling PtdIns(3,4,5) P3 levels in response to thrombin or collagen activation of mouse blood platelets.
authors.affiliation: INSERM U563, Department of Oncogenesis and Signaling in Hematopoietic Cells, IFR30, Hôpital Purpan, 31059 Toulouse Cedex, France. giuriato@stanford.edu
authors.forename: Sylvie
authors.initials: S
authors.lastname: Giuriato
authors.forename: Xavier
authors.initials: X
authors.lastname: Pesesse
authors.forename: Stéphane
authors.initials: S
authors.lastname: Bodin
authors.forename: Takehiko
authors.initials: T
authors.lastname: Sasaki
authors.forename: Cécile
authors.initials: C
authors.lastname: Viala
authors.forename: Evelyne
authors.initials: E
authors.lastname: Marion
authors.forename: Joseph
authors.initials: J
authors.lastname: Penninger
authors.forename: Stéphane
authors.initials: S
authors.lastname: Schurmans
authors.forename: Christophe
authors.initials: C
authors.lastname: Erneux
authors.forename: Bernard
authors.initials: B
authors.lastname: Payrastre
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000187:drug effects / Q000201:enzymology*; D019556:COS Cells; D045744:Cell Line, Tumor; D003599:Cytoskeleton / Q000201:enzymology; D006801:Humans; D000072181:Inositol Polyphosphate 5-Phosphatases; D051379:Mice; D018345:Mice, Knockout; D018129:Phosphatidylinositol Phosphates / Q000378:metabolism*; D000072183:Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatases; D010744:Phosphoric Monoester Hydrolases / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology*; D010766:Phosphorylation; D013917:Thrombin / Q000494:pharmacology; D014443:Tyrosine / Q000378:metabolism; D018909:src Homology Domains
chemical_list: D018129:Phosphatidylinositol Phosphates; C060974:phosphatidylinositol 3,4,5-triphosphate; D014443:Tyrosine; D010744:Phosphoric Monoester Hydrolases; D000072181:Inositol Polyphosphate 5-Phosphatases; C000606430:INPP5D protein, human; C098706:INPPL1 protein, human; C000606433:Inpp5d protein, mouse; C000606435:Inppl1 protein, mouse; D000072183:Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatases; D013917:Thrombin