Unnatural base pairs between 2-amino-6-(2-thienyl)purine and the complementary bases.
The unnatural base, 2-amino-6-(2-thienyl)purine (designated as s), instead of 2-amino-6-(N,N-dimethylamino)purine (designated as x), was designed in order to improve the specificity and efficiency of the base pairing with pyridin-2-one (designated as y). DNA fragments containing s were chemically synthesized, and the thermal stability and the enzymatic reactions involving the s-y pairing were examined. Thermal denaturation experiments showed that the DNA duplex (12-mer) containing the s-y pair was more stable than that containing the x-y pair. The incorporation of dyTP was also more advantageous to the s-y pairing than the x-y pairing in single-nucleotide insertion experiments using the Klenow fragment of Escherichia coli DNA polymerase I.
Hirao
I
I
Yokoyama CytoLogic Project, ERATO, JST, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
Fujiwara
T
T
Kimoto
M
M
Mitsui
T
T
Okuni
T
T
Ohtsuki
T
T
Yokoyama
S
S
D020029:Base Pairing; D001665:Binding Sites; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004256:DNA Polymerase I / Q000378:metabolism; D004261:DNA Replication; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004355:Drug Stability; D004926:Escherichia coli / Q000201:enzymology; D066298:In Vitro Techniques; D007700:Kinetics; D009691:Nucleic Acid Denaturation; D011687:Purines / Q000737:chemistry* / Q000378:metabolism; D014158:Transcription, Genetic; D014764:Viral Proteins
C435686:2-amino-6-(2-thienyl)purine; D011687:Purines; D014764:Viral Proteins; D004247:DNA; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases; D004256:DNA Polymerase I