A new mitochondrial fluorescent zinc sensor.
A novel cationic fluorescent zinc (Zn2+) indicator (RhodZin-3) with nanomolar affinity for Zn2+ has been synthesized. RhodZin-3 exhibits large pH-independent fluorescence increases in the orange region of the visible wavelength spectrum with increasing zinc concentrations, and no sensitivity to physiologically relevant Ca2+ concentrations. Experiments in neuronal cell cultures show that RhodZin-3 effectively localizes into mitochondria and detects changes of intramitochondrial free Zn2+ ([Zn2+]m).
Sensi
Stefano L
SL
Department of Neurology, University of California, Irvine, CA 92697-4292, USA.
Ton-That
Dien
D
Weiss
John H
JH
Rothe
Anca
A
Gee
Kyle R
KR
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D001923:Brain Chemistry; D002478:Cells, Cultured; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D051379:Mice; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000737:chemistry*; D016548:Prosencephalon / Q000166:cytology; D012680:Sensitivity and Specificity; D014966:Xanthenes / Q000138:chemical synthesis / Q000737:chemistry*; D015032:Zinc / Q000032:analysis* / Q000737:chemistry
D005456:Fluorescent Dyes; C484817:RhodZin-3; D014966:Xanthenes; D015032:Zinc