title: DNA recognition control of gamma-PRNA and mismatched base effects upon complex stability.
abstract: Oligomeric peptide ribonucleic acids (PRNA's), tethering 5'-amino-5'-deoxyribonucleosides as a recognition site for nucleic acids, has been designed and synthesized by solid phase method. PRNA 12-mer found to form stable duplex with complementary DNA.PRNA.DNA duplex composed of PRNA with mismatched DNA has 10-degree lower melting temperature than fullmatched PRNA.DNA duplex. These results demonstrate that binding of PRNA with DNA is sequence specific. It should be emphasized that the on-off switching of PRNA.DNA duplex formation has been realized by the borate added as an external factor.
authors.affiliation: Department of Molecular Chemistry, Graduate School of Engineering, Osaka University, Yamada-oka, Suita 565-0871, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Sato
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Hashimoto
authors.forename: Mayuko
authors.initials: M
authors.lastname: Kikkawa
authors.forename: Takehiko
authors.initials: T
authors.lastname: Wada
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Inoue
mesh_terms: D020137:Base Pair Mismatch; D001483:Base Sequence; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D020135:Peptide Nucleic Acids / Q000737:chemistry*
chemical_list: D020135:Peptide Nucleic Acids; D004247:DNA