title: [Default of KIAA0353 gene in varicose great saphenous vein accompanying primary deep vein valve insufficiency].
abstract: OBJECTIVE
  To screen and identify the genes related to the occurrence and development of varicose great saphenous vein in the patients with primary deep vein valve insufficiency (PDVI).
  
  
  METHODS
  mRNA fluorescent differential display (FDD) technique was used to compare the different cDNA fragments originated from differentially expressed mRNAs from the venous tissues of 10 patients with varicose great saphenous vein complicated with PDVI. Ten specimens of normal venous tissue from 10 patients dying from other diseases were used as controls. The differently expressed cDNA fragments were then re-amplified and labeled with DIG to prepare probes for later Northern blotting. Positive fragments confirmed by Northern blotting were cloned into pGEM-T easy vector and sequenced using Sanger's method. Then the sequences were compared with the data in GeneBank by BLASTN software to search for their genetic origin.
  
  
  RESULTS
  Altogether 37 differentially expressed cDNA fragments were discovered from the 2 groups, among which 30 were confirmed by Northern blotting. There was a notable 540 bp-long cDNA fragment, which was only presented in the control group, sharing 99% homology with part of the mRNA sequence of human KIAA0353 gene.
  
  
  CONCLUSIONS
  The varicose great saphenous vein of PDVI patients is a process with the involvement of multiple genes and the default of KIAA0353 gene may play a role in the occurrence and development of varicose great saphenous vein in PDVI patients.
authors.affiliation: Department of Vascular Surgery, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
authors.forename: Heng-hu
authors.initials: HH
authors.lastname: Yin
authors.forename: Shen-ming
authors.initials: SM
authors.lastname: Wang
authors.forename: Jin-song
authors.initials: JS
authors.lastname: Wang
authors.forename: Zuo-jun
authors.initials: ZJ
authors.lastname: Hu
authors.forename: Xue-ling
authors.initials: XL
authors.lastname: Huang
mesh_terms: D001483:Base Sequence; D006801:Humans; D007381:Intermediate Filament Proteins / Q000737:chemistry; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000737:chemistry; D012333:RNA, Messenger / Q000737:chemistry; D012501:Saphenous Vein / Q000378:metabolism*; D014648:Varicose Veins / Q000235:genetics*; D014689:Venous Insufficiency / Q000235:genetics*
chemical_list: D007381:Intermediate Filament Proteins; D009124:Muscle Proteins; D012333:RNA, Messenger; C430456:desmuslin