Neutrophilic transient acidification and superoxide production in peritoneal dialysate.
OBJECTIVE
To elucidate whether an inhibited superoxide production (O2-) of neutrophils induced by commercial lactate-based peritoneal dialysates (PDS) could be corrected after a transient intracellular acidosis.


METHODS
The intracellular pH ([pHi]) of human neutrophils incubated in PDS was monitored with a spectrofluorometer with a pH-sensitive dye (BCECF-AM). Neutrophilic O2- stimulated by zymosan was determined in PDS with the superoxide dismutase inhibitable ferricytochrome c reduction, using a spectrophotometer.


RESULTS
The severe intracellular acidosis induced within 5 min by PDS at an extracellular pH of 5.2 could be promptly and completely recovered by a neutralization of the pH of media. However, O2- by neutrophils exposed to the PDS for as little as 5 min was drastically and persistently inhibited, even the acidic [pHi] of cells had been fully returned for 1 h.


CONCLUSIONS
The intracellular acidification of cells in the initial phase could be transient and reversible, but impaired cell functions, at least in part including O2- generating system, might be consistent and irreversible in the early stage of the cellular acidosis in the peritoneal cavity of CAPD patients. The findings above may be of particular importance in both clinic and cell biology.
Zhou
F Q
FQ
Renal and Hypertension Section, Hines-Loyola Medical Center, Chicago, IL., USA.
Zhou
X J
XJ
Yu
A W
AW
Song
R H
RH
D000138:Acidosis / Q000209:etiology / Q000517:prevention & control; D002469:Cell Separation; D002470:Cell Survival; D015314:Dialysis Solutions / Q000009:adverse effects*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000009:adverse effects*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000378:metabolism*; D010531:Peritoneal Dialysis, Continuous Ambulatory; D013481:Superoxides / Q000378:metabolism*; D015054:Zymosan / Q000494:pharmacology
D015314:Dialysis Solutions; D013481:Superoxides; D019344:Lactic Acid; D009240:N-Formylmethionine Leucyl-Phenylalanine; D015054:Zymosan