title: Glucose uptake in germinating Aspergillus nidulans conidia: involvement of the creA and sorA genes.
abstract: D-Glucose uptake in germinating wild-type Aspergillus nidulans conidia is an energy-requiring process mediated by at least two transport systems of differing affinities for glucose: a low-affinity system (K(m) approximately 1.4 mM) and a high-affinity system (K(m) approximately 16 micro M). The low-affinity system is inducible by glucose; the high-affinity system is subject to glucose repression effected by the carbon catabolite repressor CreA and is absent in sorA3 mutant conidia, which exhibit resistance to L-sorbose toxicity. An intermediate-affinity system (K(m) approximately 400 micro M) is present in sorA3 conidia germinating in derepressing conditions. creA derepressed mutants show enhanced sensitivity to L-sorbose. The high-affinity uptake system appears to be responsible for the uptake of this toxic sugar.
authors.affiliation: Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Apartado de Correos 73, Burjassot, 46100 Valencia, Spain.
authors.forename: Andrew P
authors.initials: AP
authors.lastname: MacCabe
authors.affiliation: Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Apartado de Correos 73, Burjassot, 46100 Valencia, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Miró
authors.affiliation: Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Apartado de Correos 73, Burjassot, 46100 Valencia, Spain.
authors.forename: Luisa
authors.initials: L
authors.lastname: Ventura
authors.affiliation: Departamento de Medicina Preventiva y Salud Pública, Bromatología, Toxicología y Medicina Legal, Facultad de Farmacia, Universitat de València, Avenida Vicente Andrés Estellés s/n, 46100-Burjassot, Valencia, Spain.|Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Apartado de Correos 73, Burjassot, 46100 Valencia, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Ramón
mesh_terms: D001233:Aspergillus nidulans / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D001693:Biological Transport, Active; D025141:Drug Resistance, Fungal / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D005800:Genes, Fungal; D005947:Glucose / Q000378:metabolism*; D007700:Kinetics; D009154:Mutation; D012097:Repressor Proteins / Q000235:genetics; D013013:Sorbose / Q000494:pharmacology
chemical_list: D005656:Fungal Proteins; D012097:Repressor Proteins; C071237:CreA protein, Aspergillus nidulans; D005947:Glucose; D013013:Sorbose