Genomic analysis of mefloquine-resistant Plasmodium falciparum.
We studied sequence polymorphism, expression level of pfmdr1 gene and drug sensitivity in mefloquine sensitive-strain as well as mefloquine-resistant clone/24 to understand underlying drug resistance mechanism. Mefloquine-resistant clone/24 exhibited decreased susceptibility to mefloquine, quinine, halofantrine and artemisinin and increased susceptibility to chloroquine. We analyzed sequence of pfmdr1 gene and found mutation in pfmdr1 on clone/24. Moreover, overexpression of mRNA level of pfmdr1 has been observed in mefloquine-resistant clone/24. Our results explain gene mutation and expression level of pfmdr1 may be associated in mefloquine resistance mechanism.
Begum
Khurshida
K
Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama 700-8530, Japan.
Kim
Hye-Sook
HS
Okuda
Yohei
Y
Wataya
Yusuke
Y
Kimura
Mikio
M
Huruta
Takahisa
T
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D004351:Drug Resistance / Q000235:genetics*; D005786:Gene Expression Regulation; D018503:Genome, Protozoan; D015767:Mefloquine / Q000494:pharmacology*; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics
D000962:Antimalarials; D012333:RNA, Messenger; D015767:Mefloquine