Enhancement of the catalytic activity of an artificial phosphotriesterase using a molecular imprinting technique.
An artificial phosphotriesterase (PTE) was constructed by co-polymerization of 4(5)-vinylimidazole-Zn(2+)-methacrylic acid cluster with a divinylbenzene polymer. Compared with the spontaneous hydrolysis, the resulting polymer catalyst caused 105-fold rate acceleration towards the hydrolysis of diethyl p-nitrophenyl phosphate (Paraoxon). The catalytic activity of the polymer catalyst could be enhanced for 30% using molecular imprinting technique and the molecularly-imprinted catalyst (MIC) showed a turnover rate of 7.4 x 10(-2) s(-1) towards the hydrolysis of Paraoxon. The MIC also hydrolyzed thiophosphates and phosphorothiolate triester pesticides. Construction of an amperometric sensor employing the MIC as catalyst achieved a detection limit of 0.1 mM Paraoxon.
Meng
Zihui
Z
Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16 Nakamachi, Koganei, Tokyo 184-8588, Japan.
Yamazaki
Tomohiko
T
Sode
Koji
K
D000327:Adsorption; D043303:Aryldialkylphosphatase; D040761:Biomimetic Materials / Q000138:chemical synthesis; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002384:Catalysis; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004800:Enzymes, Immobilized / Q000138:chemical synthesis*; D004950:Esterases / Q000138:chemical synthesis* / Q000737:chemistry; D007093:Imidazoles / Q000737:chemistry; D008689:Methacrylates / Q000737:chemistry; D010261:Paraoxon / Q000737:chemistry*; D010575:Pesticides / Q000032:analysis* / Q000737:chemistry; D011786:Quality Control; D014753:Vinyl Compounds / Q000737:chemistry; D015032:Zinc / Q000737:chemistry
D004800:Enzymes, Immobilized; D007093:Imidazoles; D008689:Methacrylates; D010575:Pesticides; D014753:Vinyl Compounds; C008384:methacrylic acid; C029899:imidazole; D004950:Esterases; D043303:Aryldialkylphosphatase; C004985:divinyl benzene; D015032:Zinc; D010261:Paraoxon