title: Centaurin-alpha(1) associates with and is phosphorylated by isoforms of protein kinase C.
abstract: Centaurin-alpha(1) is a member of the family of ADP-ribosylation factors (ARF) GTPase activating proteins (GAPs), although ARF GAP activity has not yet been demonstrated. The human homologue, centaurin-alpha(1) functionally complements the ARF GAP activity of Gcs1 in yeast. Although Gcs1 is involved in the formation of actin filaments in vivo, the function of centaurin remains elusive. We have identified a number of novel centaurin-alpha(1) binding partners; including CKIalpha and nucleolin. In this report, we have focused on the interaction of centaurin-alpha(1) with PKC. All groups of PKC associate directly through their cysteine rich domains. Centaurin-alpha(1) is also a substrate for all PKC classes and we have identified the two sites of phosphorylation. This is the first report of a kinase that phosphorylates centaurin-alpha(1).
authors.affiliation: University of Edinburgh, School of Biomedical and Clinical Laboratory Sciences, Hugh Robson Building, George Square, Edinburgh EH8 9XD, UK.
authors.forename: Eva
authors.initials: E
authors.lastname: Zemlickova
authors.forename: Thierry
authors.initials: T
authors.lastname: Dubois
authors.forename: Preeti
authors.initials: P
authors.lastname: Kerai
authors.forename: Sam
authors.initials: S
authors.lastname: Clokie
authors.forename: Andy D
authors.initials: AD
authors.lastname: Cronshaw
authors.forename: Robert I D
authors.initials: RI
authors.lastname: Wakefield
authors.forename: Franz-Josef
authors.initials: FJ
authors.lastname: Johannes
authors.forename: Alastair
authors.initials: A
authors.lastname: Aitken
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D001665:Binding Sites; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000378:metabolism*; D010766:Phosphorylation; D011493:Protein Kinase C / Q000737:chemistry / Q000378:metabolism*; D017434:Protein Structure, Tertiary
chemical_list: C100956:ADAP1 protein, human; D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D007527:Isoenzymes; D009419:Nerve Tissue Proteins; C089212:protein kinase D; D011493:Protein Kinase C