title: Small intestinal submucosa in abdominal wall repair after TRAM flap harvesting in a rat model.
abstract: The strength of porcine small intestinal submucosa in abdominal wall repair after transverse rectus abdominis myocutaneous flap harvesting was examined in a rat model. Changes in the levels of selected molecular markers of inflammation after small intestinal submucosa implantation were also studied. Eighty-three rats were divided into three groups. In experimental group I, an abdominal wall defect created by removal of the rectus abdominis muscle was repaired with placement of a 1.5 x 5-cm2 patch of small intestinal submucosa. In experimental group II, the muscle defect was repaired with a combination of small intestinal submucosa patch placement and fascial closure. In the control group, the defect was repaired with direct fascial closure. At postoperative times of 3 days, 2 weeks, 1 month, and 2 months, the muscle tissues adjacent to the abdominal wall repair site were subjected to biopsies for assessment of inflammation markers. Full-thickness sections of the abdominal wall from the repair site in each animal were removed for tensile strength testing and histological examinations. The results demonstrated that interleukin-6 and interferon-gamma levels were increased in the two experimental, small intestinal submucosa-treated groups at 3 days and 2 weeks postoperatively. The results of mechanical testing demonstrated that the average tensile strength of the repaired abdominal wall in the repair model with combined small intestinal submucosa placement and fascial repair was significantly greater than the values for repairs with fascial closure or small intestinal submucosa placement alone. The use of small intestinal submucosa placement in combination with fascial repair can significantly improve the strength of the repaired abdominal wall after transverse rectus abdominis myocutaneous flap harvesting.
authors.affiliation: Department of Pediatrics, Division of Plastic Surgery, University of Mississippi Medical Center, Jackson, 39216, USA.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Shuying
authors.initials: S
authors.lastname: Lin
authors.forename: Tanya
authors.initials: T
authors.lastname: Oswald
authors.forename: William
authors.initials: W
authors.lastname: Sones
authors.forename: Zhengwei
authors.initials: Z
authors.lastname: Cai
authors.forename: Wanda
authors.initials: W
authors.lastname: Dorsett-Martin
authors.forename: William C
authors.initials: WC
authors.lastname: Lineaweaver
mesh_terms: D000818:Animals; D000071938:Fasciotomy; D007371:Interferon-gamma / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D007413:Intestinal Mucosa / Q000637:transplantation*; D007421:Intestine, Small; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017568:Rectus Abdominis / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D013524:Surgical Flaps; D013718:Tensile Strength; D020858:Tissue and Organ Harvesting
chemical_list: D007376:Interleukin-2; D015850:Interleukin-6; D007371:Interferon-gamma