Synthesis and properties of phosphorylated 3'-O-beta-D-ribofuranosyl-2'-deoxythymidine.
A strategy was developed for the synthesis of 3'-O-beta-D-ribofuranosyl 2'-deoxythymidine derivatives using three different protecting groups, which allows the synthesis of a phosphoramidite building block for oligonucleotide synthesis. Likewise the 5'-O- and 5''-O-phosphorylated analogues were synthesized and their conformation was determined using NMR spectroscopy.
Efimtseva
Ekaterina V
EV
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
Shelkunova
Alexandra A
AA
Mikhailov
Sergey N
SN
Nauwelaerts
Koen
K
Rozenski
Jef
J
Lescrinier
Eveline
E
Herdewijn
Piet
P
D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000737:chemistry; D010766:Phosphorylation; D013936:Thymidine / Q000138:chemical synthesis* / Q000737:chemistry*
D009841:Oligonucleotides; D009943:Organophosphorus Compounds; C434331:phosphoramidite; D013936:Thymidine