title: Evaluation of the improvement of IGCR technique.
abstract: We have improved the protocol of In-Gel Competitive Reassociation (IGCR) technique, one of genome subtraction methods, and developed the apparatus for this technique. The protocol obtained by the fluorescence monitor that we had reported on this symposium last year was added to the improved IGCR technique, which made IGCR method simple and less time consuming for handling. The comprehensive subtractions with genes from twins, one of whom with rheumatoid arthritis, were employed and IGCR libraries were constructed. The analysis of the library clones will be reported.
authors.affiliation: Tsukuba Research Institute, Novartis Pharma K.K., Ohkubo 8, Tsukuba 300-2611, Japan.
authors.forename: Takanori
authors.initials: T
authors.lastname: Yasukochi
authors.forename: Kazuo
authors.initials: K
authors.lastname: Ozawa
authors.forename: Masaaki
authors.initials: M
authors.lastname: Sato
authors.forename: Akito
authors.initials: A
authors.lastname: Tsutsumi
authors.forename: Takayuki
authors.initials: T
authors.lastname: Sumida
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Shibanaka
mesh_terms: D001172:Arthritis, Rheumatoid / Q000235:genetics; D004247:DNA / Q000302:isolation & purification; D004586:Electrophoresis / Q000379:methods*; D006801:Humans
chemical_list: D004247:DNA