Photo-regulation of DNA/RNA duplex formation by azobenzene-tethered DNA towards antisense strategy.
Modified DNA carrying an azobenzene was successfully applied to the photo-regulation of DNA/RNA hybridization. When the azobenzene was isomerized from trans- to cis-form on UV-irradiation, the melting temperature of the duplex was significantly lowered. This process was totally reversible so that the Tm increased by cis-->trans isomerization induced by visible light irradiation.
Liang
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X
Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguroku, Tokyo 153-8904, Japan.
Yoshida
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Asanuma
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Komiyama
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M
D001391:Azo Compounds / Q000737:chemistry; D001483:Base Sequence; D002942:Circular Dichroism; D016373:DNA, Antisense / Q000737:chemistry* / Q000235:genetics / Q000528:radiation effects; D015870:Gene Expression / Q000528:radiation effects; D066298:In Vitro Techniques; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D020319:Oligodeoxyribonucleotides, Antisense / Q000737:chemistry / Q000235:genetics / Q000528:radiation effects; D009843:Oligoribonucleotides / Q000737:chemistry / Q000235:genetics / Q000528:radiation effects; D010777:Photochemistry; D012313:RNA / Q000737:chemistry* / Q000235:genetics / Q000528:radiation effects; D013056:Spectrophotometry, Ultraviolet; D014466:Ultraviolet Rays
D001391:Azo Compounds; D016373:DNA, Antisense; D020319:Oligodeoxyribonucleotides, Antisense; D009843:Oligoribonucleotides; D012313:RNA; C009850:azobenzene