title: Expression of recombinant human ICOS and in vitro characterization of its bioactivity on B lymphocytes.
abstract: Inducible costimulator (ICOS) is a novel costimulatory molecule expressed in activated T cell and has critical regulation effect on special immune response. In this study, the cDNA encoding human ICOS was cloned from activated tonsil cells via RT-PCR, and was expressed in E. coli on pET28 expression vector. The recombinant ICOS protein expressed from E. coli showed a molecular weight of 14 kD on SDS-polyacrylamide gel electrophoresis and was further confirmed by Western blot. In presence of IL-10, the purified rhICOS significantly increased in vitro B cell growth stimulated by pokeweed mitogen (PWM), and enhanced the secretion of IgG from B cells.
authors.affiliation: Biotechnology Research Institute, Soochow University, Suzhou 215007, China. smbxuegz@public1.sz.js.cn
authors.forename: Zhong-Bin
authors.initials: ZB
authors.lastname: Deng
authors.forename: Chang-Ming
authors.initials: CM
authors.lastname: Lu
authors.forename: Wei-Da
authors.initials: WD
authors.lastname: Huang
authors.forename: Li-Qin
authors.initials: LQ
authors.lastname: Shen
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.forename: Hong-Bing
authors.initials: HB
authors.lastname: Ma
authors.forename: Pan-Sheng
authors.initials: PS
authors.lastname: Fan
authors.forename: Xue-Guang
authors.initials: XG
authors.lastname: Zhang
mesh_terms: D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D001402:B-Lymphocytes / Q000187:drug effects*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D060889:Inducible T-Cell Co-Stimulator Protein; D008213:Lymphocyte Activation / Q000187:drug effects; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; C556692:ICOS protein, human; D007074:Immunoglobulin G; D060889:Inducible T-Cell Co-Stimulator Protein; D011994:Recombinant Proteins