title: Direct PCR detection of Escherichia coli O157:H7.
abstract: OBJECTIVE
  This paper reports a simple, rapid approach for the detection of Shiga toxin (Stx)-producing Escherichia coli (STEC).
  
  
  RESULTS
  Direct PCR (DPCR) obviates the need for the recovery of cells from the sample or DNA extraction prior to PCR. Primers specific for Stx-encoding genes stx1 and stx2 were used in DPCR for the detection of E. coli O157:H7 added to environmental water samples and milk.
  
  
  CONCLUSIONS
  PCR reactions containing one cell yielded a DPCR product.
  
  
  CONCLUSIONS
  This should provide an improved method to assess contamination of environmental and other samples by STEC and other pathogens.
authors.affiliation: Department of Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, WI, USA.
authors.forename: K A
authors.initials: KA
authors.lastname: Fode-Vaughan
authors.forename: J S
authors.initials: JS
authors.lastname: Maki
authors.forename: J A
authors.initials: JA
authors.lastname: Benson
authors.forename: M L P
authors.initials: ML
authors.lastname: Collins
mesh_terms: D000818:Animals; D019453:Escherichia coli O157 / Q000235:genetics / Q000302:isolation & purification*; D005618:Fresh Water / Q000382:microbiology*; D006801:Humans; D008892:Milk / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D022622:Shiga Toxin 1 / Q000096:biosynthesis / Q000235:genetics; D022641:Shiga Toxin 2 / Q000096:biosynthesis / Q000235:genetics; D014876:Water Pollution
chemical_list: D022622:Shiga Toxin 1; D022641:Shiga Toxin 2