Differentiation and malignant suppression induced by mouse erythroid differentiation and denucleation factor on mouse erythroleukemia cells.
OBJECTIVE
To investigate the roles of mouse erythroid differentiation and denucleation factor (MEDDF), a novel factor cloned in our laboratory recently, in erythroid terminal differentiation.


METHODS
Mouse erythroleukemia (MEL) cells were transfected with eukaryotic expression plasmid pcDNA-MEDDF. Then we investigated the changes on characteristics of cell growth by analyzing cells growth rate, mitotic index and colony-forming rate in semi-solid medium. The expressions of c-myc and beta-globin genes were analysed by semi-quantitative RT-PCR.


RESULTS
MEL cells transfected with pcDNA-MEDDF showed significant lower growth rate, mitotic index, and colony-forming rate in semi-solid medium (P<0.01). The percentage of benzidine-positive cells was 32.8% after transfection. The expression of beta-globin in cells transfected with pcDNA-MEDDF was 3.43 times higher than that of control (MEL transfected with blank vector, pcDNA3.1), and the expression of c-myc decreased by 66.3%.


CONCLUSIONS
MEDDF can induce differentiation of MEL cell and suppress its malignancy.
Daishu
Han
H
Department of Cell Biology, Institute of Basic Medical Sciences, CAMS & PUMC, Beijing 100005. daishu@pubic.bta.net.cn
Qing
Zhao
Z
Yehua
Ge
G
Jianping
Zhou
Z
Jing
Ma
M
Kequan
Chen
C
Shepu
Xue
X
D028341:Activins / Q000235:genetics / Q000494:pharmacology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D005622:Friend murine leukemia virus; D005914:Globins / Q000096:biosynthesis* / Q000235:genetics; D028322:Inhibin-beta Subunits / Q000235:genetics / Q000494:pharmacology*; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D014162:Transfection; D014407:Tumor Cells, Cultured
D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; C483799:erythroid differentiation and denucleation factor, mouse; D028341:Activins; D005914:Globins; D028322:Inhibin-beta Subunits