[Identification and cloning of a novel full ORF gene of P. falciparum PDZ domain containing protein].
OBJECTIVE
To identify and clone a novel full ORF gene of P. falciparum PDZ domain containing protein (PfPCP) and study its expression at the erythrocytic stage.


METHODS
The primers were designed according to the sequences from P. falciparum genomic database. The full ORF gene of PfPCP was amplified from mRNA of the erythrocytic stage of P. falciparum Hainan strain by RT-PCR. Bio-informatics software were used to analyze PfPCP; PfPCP expression at the erythrocytic stage was studied with Western Blotting.


RESULTS
The full ORF gene of PfPCP was 2,076 bp and encoded 691 amino acids. PfPCP contained the typical PDZ domain. Western blotting showed that PfPCP was expressed at the schizont sub-stage of P. falciparum erythrocytic stage.


CONCLUSIONS
The novel full ORF gene of PfPCP is cloned. As a PDZ domain containing protein, PfPCP is expressed at the schizont sub-stage of P. falciparum.
Li
H L
HL
Department of Etiology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China.
Wang
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004912:Erythrocytes / Q000469:parasitology; D017125:Genes, Protozoan / Q000235:genetics*; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000235:genetics* / Q000302:isolation & purification; D015800:Protozoan Proteins / Q000235:genetics*; D011817:Rabbits
D015800:Protozoan Proteins