[Modulation of Fcgamma and C3b receptor expression by marine bioglycans in mouse splenocytes].
Investigation of polysacharide immunomodulators of marine origin was performed--mitilane, alpha-1,4;1,6-D-glucane, isolated from midia Crenomytilus grayanus, and translam--beta-1,3;1,6-D-glucane isolated from marine algae Laminaria cichorioides were compared. Mechanisms of phagocytes cells activation were investigated. Dose-dependent ability of investigated bioglycanes to facilitate Fc gamma R [symbol: see text] C3bR expression at mice splenocytes was demonstrated in vivo and in vitro. The effect depended on immunomodulator type, incubation conditions, dose, period of incubation in vitro and by splenocytes population used for Fc gamma R and C3bR identification. It was shown that C3bR expression was more enhanced by immunomodulators than Fc gamma R expression. For Fc gamma R induction on lymphocytes membranes the presence of phagocytes cell (macrophages and neutrophils) is obligatory. Mitilane, containing alpha-1,4;1,6-D-glucane and some amount of protein is more effective in stimulation of membrane receptors expression than translam--beta-1,3;1,6-D-glucane. The results of investigation demonstrates the possibility to use marine bioglicanes as activators of Fc gamma R and C3bR activity, that is the base for control of pathological processes, related to immune system.
Zaporozhets
T S
TS
Research Institute of Epidemiology and Microbiology, Sibirian Branch of Russian Academy of Medical Sciences, Vladivostok.
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D049872:Bivalvia; D005936:Glucans / Q000494:pharmacology*; D066298:In Vitro Techniques; D007795:Laminaria; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D017463:Receptors, Complement 3b / Q000096:biosynthesis / Q000187:drug effects*; D017452:Receptors, IgG / Q000096:biosynthesis / Q000187:drug effects*; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D000276:Adjuvants, Immunologic; D005936:Glucans; D017463:Receptors, Complement 3b; D017452:Receptors, IgG; C033671:epiglucan; C477503:mitilane