title: [Optimization of the conditions of immobilization of enzymes in a photopolymeric membrane].
abstract: The residual activity of enzymes immobilized in the membrane on the basis on 1-vinyl-2-pyrrolidinone as photopolymerizable composition is studied. It is established, that under conditions of the immobilization at 20 degrees C the residual activity glucoseoxidase is about 35% from a initial level, horseredish peroxidase and urease from Jeack beans--42% and 20%, respectively. In case of an immobilization of beta-glucoseoxidase -50 degrees C it reaches almost 50% from a initial level. It was investigated the influence of different sources of UV-radiation and different substances on stability of the enzymes in the composition and in the immobilization matrix at storage. Dynamic of changes of enzyme activity at the photoimmobilization was characterized, and also the requirements for providing of its maximal storage was selected.
authors.affiliation: Palladin Institute of Biochemistry, NAS of Ukraine, Kyiv.
authors.forename: A V
authors.initials: AV
authors.lastname: Rebriev
authors.forename: N F
authors.initials: NF
authors.lastname: Starodub
authors.forename: A F
authors.initials: AF
authors.lastname: Masliuk
mesh_terms: D004356:Drug Storage; D004795:Enzyme Stability / Q000528:radiation effects; D004800:Enzymes, Immobilized / Q000378:metabolism* / Q000528:radiation effects; D007887:Fabaceae / Q000201:enzymology; D005949:Glucose Oxidase / Q000378:metabolism / Q000528:radiation effects; D008567:Membranes, Artificial; D010544:Peroxidases / Q000378:metabolism / Q000528:radiation effects; D010777:Photochemistry; D011108:Polymers; D014510:Urease / Q000378:metabolism / Q000528:radiation effects
chemical_list: D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D011108:Polymers; D005949:Glucose Oxidase; D010544:Peroxidases; D014510:Urease