Synthetic methyl hexagalacturonate hapten inhibitors of anti-homogalacturonan monoclonal antibodies LM7, JIM5 and JIM7.
A range of synthetic methyl hexagalacturonates were used as potential hapten inhibitors in competitive-inhibition enzyme-linked immunosorbent assays (ELISAs) with anti-homogalacturonan monoclonal antibodies LM7, JIM5 and JIM7. The selective inhibition of these antibodies by different haptens provides insight into the structures of the partially methyl-esterified pectin epitopes of these widely used monoclonal antibodies.
Clausen
Mads H
MH
Centre for Plant Sciences, University of Leeds, Leeds LS2 9JT, UK.
Willats
William G T
WG
Knox
J Paul
JP
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001667:Binding, Competitive; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry; D006241:Haptens / Q000737:chemistry / Q000494:pharmacology; D008745:Methylation; D010368:Pectins / Q000138:chemical synthesis* / Q000276:immunology / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006241:Haptens; D010368:Pectins; C003181:polygalacturonic acid