title: Methylation of p16 and p15 genes in multiple myeloma.
abstract: OBJECTIVE
  To investigate the frequency of p16 and p15 gene methylation in multiple myeloma (MM), and its relationship with bone marrow cell apoptosis and clinical outcome.
  
  
  METHODS
  Twenty-two patients with MM were studied to detect p16 and p15 gene methylation. Methylation-specific polymerase chain reaction (MSP) was used to detect gene methylation, and terminal transferase-mediated dUTP nick end-labeling (TUNEL) was used to detect cell apoptosis.
  
  
  RESULTS
  p16 and/or p15 gene methylatoin was detected in 10 of 22 patients (45.4%). There were 3 patients with p16 gene methylation, 9 patients with p15 gene methylation, and 2 patients with both genes methylation. The incidence of methylation of p15 gene was higher than that of p16 gene (P < 0.05). The patients with p16 and/or p15 gene methylation had a delayed cell apoptosis, poor response to chemotherapy, and a short over-all survival (OS).
  
  
  CONCLUSIONS
  The methylation of p16 and/or p15 gene plays a key role in MM apoptosis pathogenesis. The patients with both p16 and p15 gene methylation had a poor prognosis.
authors.affiliation: Department of Medicine, Beijing Chaoyang Hospital Affiliated to Capital University of Medical Sciences, Beijing 100020.
authors.forename: Wenming
authors.initials: W
authors.lastname: Chen
authors.forename: Yin
authors.initials: Y
authors.lastname: Wu
authors.forename: Jiazhi
authors.initials: J
authors.lastname: Zhu
authors.forename: Jingzhong
authors.initials: J
authors.lastname: Liu
authors.forename: Shuzhen
authors.initials: S
authors.lastname: Tan
authors.forename: Chengqing
authors.initials: C
authors.lastname: Xia
mesh_terms: D017209:Apoptosis; D018797:Cell Cycle Proteins; D050762:Cyclin-Dependent Kinase Inhibitor p15; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics; D019175:DNA Methylation; D004273:DNA, Neoplasm / Q000235:genetics; D020868:Gene Silencing; D019942:Genes, p16; D006801:Humans; D009101:Multiple Myeloma / Q000235:genetics* / Q000473:pathology; D011379:Prognosis; D014157:Transcription Factors / Q000235:genetics*; D025521:Tumor Suppressor Proteins
chemical_list: C493966:CDKN2B protein, human; D018797:Cell Cycle Proteins; D050762:Cyclin-Dependent Kinase Inhibitor p15; D019941:Cyclin-Dependent Kinase Inhibitor p16; D004273:DNA, Neoplasm; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins