title: Detection of NO-induced DNA lesions by the modified aldehyde reactive probe (ARP) assay.
abstract: When DNA is exposed to NO or HNO2, oxanine (Oxa) is formed as a major guanine lesion. For highly sensitive detection of Oxa using ARP, a probe molecule for DNA damage detection, the reactivity of ARP to Oxa was examined. Oxa site-specifically embedded in an oligonucleotide reacted with ARP but it took relatively long time (ca. 24 h) to completely convert Oxa to an ARP-labeled form.
authors.affiliation: Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima 739-8526, Japan.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Nakano
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Terato
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Yoshioka
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Ohyama
authors.forename: Kihei
authors.initials: K
authors.lastname: Kubo
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ide
mesh_terms: D000447:Aldehydes / Q000737:chemistry*; D004249:DNA Damage / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel; D015335:Molecular Probes; D009569:Nitric Oxide / Q000502:physiology*
chemical_list: D000447:Aldehydes; D015335:Molecular Probes; D009569:Nitric Oxide