title: Molecular recognition of threonine tRNA by threonyl-tRNA synthetase from an extreme thermophilic archaeon, Aeropyrum pernix K1.
abstract: To investigate the recognition sites of tRNA(Thr) for threonyl-tRNA synthetase (ThrRS) from an extreme thermophilic and aerobic archaeon, Aeropyrum pernix K1, threonylation experiments using various in vitro mutant transcripts of tRNA(Thr) were examined. The results indicated that A. pernix ThrRS did recognize the first three base pairs of acceptor stem in addition to the second and the third letters of anticodon of tRNA(Thr), in spite of its N-terminal truncated unique structure. Discriminator base was not involved in recognition by A. pernix ThRS. These determinants were confirmed by the identity switching experiments from the in vitro mutants of A. pernix tRNA(Pro) and tRNA(Asn).
authors.affiliation: Department of Material and Biological Chemistry, Faculty of Science, Yamagata University, Yamagata 990-8560, Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Nagaoka
authors.forename: Junji
authors.initials: J
authors.lastname: Yokozawa
authors.forename: Takuya
authors.initials: T
authors.lastname: Umehara
authors.forename: Jun
authors.initials: J
authors.lastname: Iwaki
authors.forename: Koji
authors.initials: K
authors.lastname: Okamoto
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Kawarabayasi
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Koyama
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Sako
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Wakagi
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kuno
authors.forename: Tsunemi
authors.initials: T
authors.lastname: Hasegawa
mesh_terms: D001105:Archaea / Q000201:enzymology*; D012363:RNA, Transfer, Thr / Q000235:genetics / Q000378:metabolism*; D013914:Threonine-tRNA Ligase / Q000378:metabolism*
chemical_list: D012363:RNA, Transfer, Thr; D013914:Threonine-tRNA Ligase