[Differentiation of natural killer cells into two functional subsets: NKh1 and NKh2].
OBJECTIVE
To verify the presence of functional subsets of natural killer cells based on the cytokine production.


METHODS
NK cells were purified and cultured in complete RPMI1640 medium in the presence of either IFN gamma + anti-IL-4(classical Th1 polarization) or IL-4 + anti-IFN gamma (classical Th2 polarization) for three days, and then were collected and detected for type I/type II cytokines by RT-PCR method.


RESULTS
NK cells were purified from 15 healthy donors, over 70% purity of NK cells were determined by flow cytometry. NK cells in peripheral blood expressed high level of type I cytokines, mainly IFN gamma, but low level of type II cytokines such as IL-10 and IL-13, IL-4 was not produced by NK cells. Cells cultured in IFN gamma + anti-IL-4 condition exhibited significantly increased level of IFN gamma, unchanged IL-2, and decreased type II cytokines. Cells grew in IL-4 + anti-IFN gamma condition exhibited increased IL-10 and IL-13, and decreased IFN gamma expressions.


CONCLUSIONS
Based on the cytokine production, NK cells may be divided into two functional subsets in the same manner as that of T lymphocytes(e.g. Th1/Th2): NKh1 and NKh2. The biological characterization and phenotypic marker are under investigate.
Liang
S J
SJ
Acadamy of Shandong Medical Sciences, Institute of Basic Research, Shandong Cancer Biotherapy Center, Jinan 250062, China.
Xu
T
T
Wei
H M
HM
Zhang
C
C
Fang
J
J
Sun
R
R
Tian
Z G
ZG
D002478:Cells, Cultured; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism*; D016753:Interleukin-10 / Q000378:metabolism; D018793:Interleukin-13 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D007694:Killer Cells, Natural / Q000145:classification* / Q000378:metabolism; D018417:Th1 Cells / Q000502:physiology; D018418:Th2 Cells / Q000502:physiology
D018793:Interleukin-13; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma