title: [Cloning, expression and purification of neural specific HuD cDNA].
abstract: OBJECTIVE
  To prokaryoticly express and purify HuD protein and its RNA recognition motifs.
  
  
  METHODS
  HuD protein was prokaryoticly expressed and purified by molecular cloning technology. Its biologic activity was testified by Western Blot.
  
  
  RESULTS
  Purified HuD protein and its RNA recognized motifs were observed.
  
  
  CONCLUSIONS
  The result might aid for basic research and clinical application.
authors.affiliation: Department of Neurology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
authors.forename: Jian-hua
authors.initials: JH
authors.lastname: Chen
authors.forename: Xiu-qin
authors.initials: XQ
authors.lastname: Liu
authors.forename: Yu-pu
authors.initials: YP
authors.lastname: Guo
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Hu
authors.forename: Jian-gang
authors.initials: JG
authors.lastname: Yuan
authors.forename: Bo-qin
authors.initials: BQ
authors.lastname: Qiang
authors.forename: Xiao-zhong
authors.initials: XZ
authors.lastname: Peng
mesh_terms: D000974:Antibodies, Antinuclear / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D018288:Carcinoma, Small Cell / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D051959:ELAV Proteins; D000067779:ELAV-Like Protein 4; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D009474:Neurons / Q000276:immunology; D020361:Paraneoplastic Syndromes, Nervous System / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000974:Antibodies, Antinuclear; D018076:DNA, Complementary; D051959:ELAV Proteins; D000067779:ELAV-Like Protein 4; C498010:ELAVL4 protein, human; D009419:Nerve Tissue Proteins; D016601:RNA-Binding Proteins