title: BHK cell attachment and growth on EDA-plasma-modified poly(L-lactide/epsilon-caprolactone) biodegradable films.
abstract: In this study, attachment and growth of Baby Hamster Kidney (BHK) cells on ethylene diamine (EDA)-plasma-treated poly(L-lactide/epsilon-caprolactone) biodegradable copolymer films were investigated. The co-polymer (Mw: 58000; Mn: 35000 and PI 1.60) was synthesised by ring-opening polymerization of the respective dimers with using stannous octoate as the catalyst. The final ratio of L-lactide to epsilon-caprolactone obtained by 1H-NMR was 87:13. The co-polymer films were treated with the EDA-plasma in a glow-discharge apparatus. The BHK-30 cell line was cultured on plain and EDA-plasma-treated films and their pre-wetted forms (with ethanol and/or cell culture medium before use). Cell attachment and growth were followed. Alkaline phosphatase (ALP) activity and glucose uptake in cell culture medium were also investigated. There was no attachment in the first 12 h. Glow-discharge treatment increased significantly the attachment and growth. Pre-wetting with ethanol and cell culture medium was also increase significantly both the attachment and growth.
authors.affiliation: Hacettepe University, Faculty of Science, Department of Biology, Beytepe, 06532 Ankara, Turkey.
authors.forename: O Aylin
authors.initials: OA
authors.lastname: Gürpinar
authors.forename: Kadriye
authors.initials: K
authors.lastname: Tuzlakoğlu
authors.forename: M Ali
authors.initials: MA
authors.lastname: Onur
authors.forename: Aşkin
authors.initials: A
authors.lastname: Tümer
authors.forename: Muhittin A
authors.initials: MA
authors.lastname: Serdar
authors.forename: Nilay
authors.initials: N
authors.lastname: Unal
authors.forename: Erhan
authors.initials: E
authors.lastname: Pişkin
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry*; D002448:Cell Adhesion; D002455:Cell Division; D002460:Cell Line; D006224:Cricetinae; D003470:Culture Media; D004847:Epithelial Cells / Q000378:metabolism*; D000431:Ethanol / Q000737:chemistry; D005029:Ethylenediamines / Q000737:chemistry*; D005947:Glucose / Q000493:pharmacokinetics; D007526:Isoelectric Point; D007668:Kidney / Q000166:cytology; D007783:Lactones / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008567:Membranes, Artificial; D008970:Molecular Weight; D011091:Polyesters / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D013997:Time Factors
chemical_list: D001672:Biocompatible Materials; D003470:Culture Media; D005029:Ethylenediamines; D007783:Lactones; D008567:Membranes, Artificial; D011091:Polyesters; D011108:Polymers; D000431:Ethanol; C031234:ethylenediamine; D000469:Alkaline Phosphatase; D005947:Glucose