title: [Establishment and preliminary application of screening methods for Y chromosome microdeletions in male infertility patients].
abstract: OBJECTIVE
  To develop a multiplex PCR protocol, which could be suitable for routine screening of microdeletions on the Y chromosome in azoospermic and oligozoospermic male infertility patients.
  
  
  METHODS
  Five multiplex sets were established. Eighty-seven azoospermic and oligozoospermic patients undergoing intracytoplasmic sperm injection (ICSI) in the in vitro fertilization (IVF) center and 30 azoospermic men undergoing testicular biopsy in the clinic of Urology Surgery were screened for microdeletions of Y chromosome.
  
  
  RESULTS
  A total of 19 (16.2%) cases of microdeletions were found in 117 azoospermic and oligozoospermic patients by screening of Y chromosome microdeletions. Of these, 11 cases (18.0%) were found in 61 oligozoospermic patients, and 8 cases (14.3%) were found in 56 azoospermic patients.
  
  
  CONCLUSIONS
  The multiplex PCR protocol presented in this study is an easy-to-do and reliable method for detecting microdeletions on the Y chromosome. Routine screening of microdeletions on the Y chromosome for azoospermic and oligozoospermic patients is essential.
authors.affiliation: Department of Obstetrics and Gynecology, Reproductive Medical Center, the Second Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, 510120 PR China. yucongyi01@sohu.com
authors.forename: Cong-yi
authors.initials: CY
authors.lastname: Yu
authors.forename: Guang-lun
authors.initials: GL
authors.lastname: Zhuang
authors.forename: Can-quan
authors.initials: CQ
authors.lastname: Zhou
authors.forename: Zong-he
authors.initials: ZH
authors.lastname: Yan
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Hua
authors.initials: H
authors.lastname: Gao
authors.forename: De-rong
authors.initials: DR
authors.lastname: Rui
mesh_terms: D002872:Chromosome Deletion; D041322:Chromosomes, Human, Y / Q000235:genetics*; D005260:Female; D005820:Genetic Testing / Q000379:methods; D006801:Humans; D007248:Infertility, Male / Q000175:diagnosis / Q000235:genetics*; D008297:Male; D016133:Polymerase Chain Reaction