title: [Study on DNA sequences of Paragonimus skrjabini populations from five provinces in China].
abstract: OBJECTIVE
  To study differences among Paragonimus skrjabini populations from five provinces (Guangdong, Fujian, Yunnan, Hubei, Sichuan) and Paragonimus szechuanensis and to analyze the taxonomic status of P. heterotremus and P. veocularis in the Genus Paragonimus Braun, 1899.
  
  
  METHODS
  DNA sequences were obtained from the ITS2 and CO1 genes and phylogenetic trees were built.
  
  
  RESULTS
  Difference of the DNA sequences among P. skrjabini populations from five provinces were minor. P. veocularis and P. skrjabini were similar in DNA sequences. There were also some resemblances between P. miyazakii from Japan and the Fujian isolates of P. skrjabin. In addition, P. heterotremus was found to be closer to P. skrjabini and quite a distance from P. westermani in the phylogenetic tree.
  
  
  CONCLUSIONS
  All studied populations can be regarded as different strains of P. skrjabini; P. szechuanensis is not a separate species, but possibly a geographical strain of P. skrjabini. Also found was that P. veocularis and P. miyazakii may be the synonyms of P. skrjabini. As a separate species, P. heterotremus was found to be closer to P. skrjabini and quite a distance from P. westermani in the genetic relationship.
authors.affiliation: Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025.
authors.forename: Ai-li
authors.initials: AL
authors.lastname: Cui
authors.forename: Zheng-shan
authors.initials: ZS
authors.lastname: Chang
authors.forename: Ming-gang
authors.initials: MG
authors.lastname: Chen
authors.forename: David
authors.initials: D
authors.lastname: Blair
authors.forename: Yong-nian
authors.initials: YN
authors.lastname: Zhang
authors.forename: Shao-hong
authors.initials: SH
authors.lastname: Chen
authors.forename: Zheng
authors.initials: Z
authors.lastname: Feng
mesh_terms: D000818:Animals; D001483:Base Sequence; D002681:China; D018509:DNA, Helminth / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D008969:Molecular Sequence Data; D010238:Paragonimus / Q000145:classification* / Q000235:genetics*; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D018509:DNA, Helminth; D004275:DNA, Ribosomal; D003576:Electron Transport Complex IV