[Study on transfer of interleukin-7 gene into ovarian carcinoma cells in vitro].
OBJECTIVE
To investigate proliferative activity, expression of cell surface antigens and cytokines of ovarian carcinoma cells transferred by interleukin-7 (IL-7) gene, and its cytotoxic sensitivity to lymphokine activated killer (LAK) cells in vitro through gene transfection technique.


METHODS
Ovarian carcinoma cell line SKOV3 were cultured and transferred by IL-7 cDNA. The proliferation activity of IL-7 gene transferred and nontransferred SKOV3 was observed with inverse phase-contrast light microscope, thiazoyl blue tetrazolium bromide assay and flow cytometry. The expressions of cell surface antigen human leucocyte antigen-ABC (HLA-ABC), human leucocyte antigen-DR (HLA-DR) and intercellular adhesion molecule-I (ICAM-I) were detected by indirect targeted FITC- flow cytometry. The secretions of IL-2, tumor necrosis factor alpha (TNF-alpha) and transforming growth factor (TGF-beta(1)) were assayed by enzyme linked immunosorbent assay. The cytotoxic sensitivity against LAK was detected by LDH release method.


RESULTS
The morphology, proliferation, cell cycle distributions and HLA-ABC, HLA-DR expression of IL-7 gene transferred SKOV3 didn't change after IL-7 gene transfer. The expression of ICAM-I in IL-7 gene transferred SKOV3 significantly increased and TGF-beta(1) secretion of IL-7 gene transferred SKOV3 significantly decreased. The cytotoxic sensitivity of IL-7 gene transferred SKOV3 to LAK significantly elevated.


CONCLUSIONS
Ovarian carcinoma cell line SKOV3, after transferred by IL-7 gene, remains unchanged proliferation and apoptosis, upregulation of ICAM-I, downregulation of TGF-beta(1) and increases the cytotoxic sensitivity to LAK in vitro.
Cheng
Bei
B
Department of Gynecological Tumor, Hospital of Obstetrics and Gynecology, School of Medicine, Zhejiang University, Hangzhou 310006, China.
Chen
Huai-zeng
HZ
Ye
Da-feng
DF
Lu
Wei-guo
WG
Xie
Xing
X
D000954:Antigens, Surface / Q000096:biosynthesis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D015851:Interleukin-7 / Q000235:genetics* / Q000276:immunology; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D014162:Transfection
D000954:Antigens, Surface; D016207:Cytokines; D015395:Histocompatibility Antigens Class I; D015851:Interleukin-7; D018799:Intercellular Adhesion Molecule-1