CIITA-regulated plexin-A1 affects T-cell-dendritic cell interactions.
The major histocompatibility complex (MHC) class II transactivator (CIITA) is the 'master coactivator' of MHC class II genes. To identify new targets of CIITA, we analyzed cDNA microarrays of dendritic cells (DCs) from CIITA-deficient, MHC class II-deficient and control mice. We found the semaphorin receptor plexin-A1 was expressed in DCs, but not in other immune cells, and was strongly induced by CIITA. RNA interference by short hairpin RNA specific for plexin-A1, but not a single-nucleotide mutant, greatly reduced plexin-A1 expression and T cell stimulation by protein- or peptide-antigen-pulsed DCs.Plexin-A1 is not required for peptide binding to MHC. These data indicate involvement of plexin-A1 in T cell-DC interactions but not antigen processing or binding.
Wong
Athena W
AW
Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA.
Brickey
W June
WJ
Taxman
Debra J
DJ
van Deventer
Hendrick W
HW
Reed
William
W
Gao
Jian Xin
JX
Zheng
Pan
P
Liu
Yang
Y
Li
Ping
P
Blum
Janice S
JS
McKinnon
Karen P
KP
Ting
Jenny P-Y
JP
D000818:Animals; D001483:Base Sequence; D002450:Cell Communication / Q000276:immunology*; D003433:Crosses, Genetic; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D015536:Down-Regulation / Q000276:immunology; D005434:Flow Cytometry; D005802:Genes, MHC Class II; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000276:immunology*; D009687:Nuclear Proteins; D020411:Oligonucleotide Array Sequence Analysis; D011401:Promoter Regions, Genetic; D011956:Receptors, Cell Surface / Q000235:genetics / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D015534:Trans-Activators / Q000276:immunology*; D015533:Transcriptional Activation / Q000276:immunology
D018076:DNA, Complementary; C083728:MHC class II transactivator protein; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; C492114:Plxna1 protein, mouse; D011956:Receptors, Cell Surface; D015534:Trans-Activators