Support for a meiotic recombination initiation complex: interactions among Rec102p, Rec104p, and Spo11p.
Initiation of meiotic recombination in the yeast Saccharomyces cerevisiae requires at least 10 gene products. The initiation event creates double-strand breaks, which are then processed by other recombination enzymes. A variety of classical observations, such as the existence of recombination nodules, have suggested that the proteins catalyzing recombination form a complex. A variety of lines of evidence indicate that Rad50p, Mre11p, and Xrs2p interact, and genetic data suggesting interactions between Rec102p and Rec104p have been reported. It has recently been shown that Spo11p coimmunoprecipitates with Rec102p in meiosis as well. In this paper, we provide genetic and biochemical evidence that the meiosis-specific proteins Rec102p, Rec104p, and Spo11p all interact with each other in meiosis. Furthermore, we demonstrate that the interaction between Rec102p and Spo11p does not require Rec104p. Likewise, the interaction between Rec104p and Rec102p does not require Spo11p, although Spo11p may stabilize that association. The interactions suggest that Spo11p, Rec102p, and Rec104p may form a trimeric complex during the initiation of recombination.
Jiao
Kai
K
Department of Biological Sciences, University of Iowa, Iowa City, Iowa 52242, USA.
Salem
Laura
L
Malone
Robert
R
D000483:Alleles; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004706:Endodeoxyribonucleases; D004950:Esterases / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism; D008540:Meiosis; D008954:Models, Biological; D016297:Mutagenesis, Site-Directed; D011233:Precipitin Tests; D011485:Protein Binding; D045522:Recombinases; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D013997:Time Factors; D020798:Two-Hybrid System Techniques; D000099257:Meiotic Recombination Protein SPO11
D004268:DNA-Binding Proteins; D004706:Endodeoxyribonucleases; D004950:Esterases; D005982:Glutathione Transferase; D045522:Recombinases; D029701:Saccharomyces cerevisiae Proteins; D000099257:Meiotic Recombination Protein SPO11; C073372:REC102 protein, S cerevisiae; C080594:REC104 protein, S cerevisiae