title: Characteristics of collagenolytic enzymes secreted by deuteromycete fungi Aspergillus flavus.
abstract: Collagen induced the synthesis of collagenolytic enzymes in the culture of Aspergillus flavus. Enzyme activity of the culture increased during storage and passage on a medium with this inductor. We developed a method for isolation and purification of collagenolytic enzymes and obtained two electrophoretically homogenous enzyme preparations belonging to neutral thermolabile collagenolytic metalloproteinases.
authors.affiliation: Research and Training and Methodical Center for Biomedical Technologies VILAR, Moscow.
authors.forename: E A
authors.initials: EA
authors.lastname: Sukhosyrova
authors.forename: Z K
authors.initials: ZK
authors.lastname: Nikitina
authors.forename: M B
authors.initials: MB
authors.lastname: Yakovleva
authors.forename: E V
authors.initials: EV
authors.lastname: Veshchikova
authors.forename: V A
authors.initials: VA
authors.lastname: Bykov
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D001231:Aspergillus flavus / Q000201:enzymology*; D018929:Cell Culture Techniques / Q000379:methods; D002614:Chelating Agents / Q000737:chemistry; D003094:Collagen / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D004492:Edetic Acid / Q000737:chemistry; D010450:Endopeptidases / Q000302:isolation & purification / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism
chemical_list: D000596:Amino Acids; D002614:Chelating Agents; D003470:Culture Media; D013395:Sucrose; D003094:Collagen; D004492:Edetic Acid; D010450:Endopeptidases