Development and application of siRNA expression vector.
RNA interference (RNAi) is a sequence-specific silencing phenomenon, which is induced by double-stranded RNA (dsRNA) and mediated through an evolutionarily conserved mechanism from plants to mammals. In mammalian cells, it has recently been reported that 21- or 22-nucleotide (nt) RNAs with 2-nt 3' overhangs (siRNA) induce RNAi without induction of the dsRNA-dependent inhibition of protein synthesis, known as the host defense system against viral infections. Moreover, we and other have developed siRNA expression systems utilizing a pol III promoter. Here we report a comparative analysis among various siRNA expression vectors and also demonstrate a regulatable RNAi in cells by using a tetracycline-controlled U6 promoter.
Miyagishi
Makoto
M
Department of Chemistry and Biotechnology, School of Engineering, University of Tokyo, Hongo, Tokyo 113-8656, Japan.
Taira
Kazunari
K
D005822:Genetic Vectors; D011401:Promoter Regions, Genetic; D034741:RNA, Small Interfering / Q000235:genetics*; D013754:Tetracyclines / Q000737:chemistry; D015854:Up-Regulation
D034741:RNA, Small Interfering; D013754:Tetracyclines