Comparison of morphological characteristics of Streptomyces natalensis by image analysis and focused beam reflectance measurement.
A morphological interpretation is presented for data collected during growth of a filamentous organism, using a focused beam reflectance measurement (FBRM) system. The morphology of the organism was also obtained using conventional semiautomatic image analysis to support the interpretation of the FBRM data. The model organism employed is the filamentous soil-borne actinomycete Streptomyces natalensis, which produces the antifungal agent pimaricin. The organism was cultivated both in shake flasks and in a bench-scale stirred tank bioreactor. It was found that FBRM could be used to track changes in the morphology of the organism throughout the course of its growth on both scales. These changes were highlighted using both the median chord length and length-weighted mean chord length obtained from the chord length distribution measured with the FBRM probe. The ability of the FBRM probe to respond to changes in both the size and morphology of mycelial aggregates was supported by standard image analysis parameters, including equivalent diameter, convex area, and compactness.
Pearson
Alan P
AP
Department of Chemical Engineering, University College Dublin, Belfield, Dublin 4, Ireland.
Glennon
Brian
B
Kieran
Patricia M
PM
D019149:Bioreactors / Q000145:classification / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002455:Cell Division / Q000502:physiology; D048429:Cell Size / Q000502:physiology*; D019544:Equipment Failure Analysis; D005240:Feasibility Studies; D007090:Image Interpretation, Computer-Assisted; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods; D009024:Morphogenesis / Q000502:physiology; D025282:Mycelium / Q000166:cytology / Q000254:growth & development; D010865:Pilot Projects; D013302:Streptomyces / Q000166:cytology* / Q000254:growth & development*; D014159:Transducers