title: [Intensification of the exopolysaccharide synthesis by Acinetobacter sp. on an ethanol-glucose mixture: aspects related to biochemistry and bioenergetics].
abstract: The possibility of intensifying the synthesis of microbial exopolysaccharides (EPS) by a strain of Acinetobacter sp. grown on a mixture of two substrates nonequivalent in terms of bioenergetics (ethanol + glucose) was shown. Based on theoretical calculations of the energy requirements for biomass and EPS synthesis from the energy-deficient substrate (glucose), the supplementary concentration of the energy-excessive substrate (ethanol) was determined that prevents the loss of glucose carbon that occurs when glucose is oxidized to CO2 to obtain energy for the processes of constructive metabolism. This made it possible to increase the efficiency of conversion of the substrate carbon to EPS. The introduction of ethanol into glucose-containing medium at a molar ratio of 3.1:1 allowed the amount of the EPS synthesized to be increased 1.8- to 1.9-fold; their yield relative to biomass increased 1.4- to 1.7-fold, and the yield of EPS relative to the substrate consumed increased 1.5- to 2-fold as compared to growth of the producer on single substrates. These results form the basis for the development of new technologies for obtaining secondary metabolites of practical value with the use of mixed growth substrates.
authors.affiliation: Zabolotnyi Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, ul. Zabolotnogo 154, Kiev, Ukraine. pirog@serv.imv.kiev.ua
authors.forename: T P
authors.initials: TP
authors.lastname: Pirog
authors.forename: M A
authors.initials: MA
authors.lastname: Kovalenko
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Kuz'minskaia
mesh_terms: D000150:Acinetobacter / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media; D004734:Energy Metabolism; D000431:Ethanol / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D013379:Substrate Specificity
chemical_list: D003470:Culture Media; D011135:Polysaccharides, Bacterial; D000431:Ethanol; D005947:Glucose