title: IL1-beta and TNF-alpha induce changes in the nuclear polyphosphoinositide signalling system in osteoblasts similar to that occurring in patients with rheumatoid arthritis: an immunochemical and immunocytochemical study.
abstract: Osteoarthritis (OA) and rheumatoid arthritis (RA) are common joint diseases that can lead to destruction of cartilage and structural changes in the subchondral bone. In this study we show by western blot and quantitative immunocytochemistry that nuclear phospholipase C beta(1) (PLC beta(1)) and phosphatidylinositol 4,5-bisphosphate (PIP(2)), two key elements of the polyphosphoinositide signal transduction system that regulate different cellular processes, increase in primary osteoblast cultures of RA patients when compared with post-traumatic after fall (PT) patients, whilst those of OA are not significantly affected. Moreover, we demonstrate that these alterations could be induced in PT osteoblasts by proinflammatory cytokines IL-1 beta and TNF-alpha. This suggests that proinflammatory cytokines, highly produced by RA infiltrating mononuclear cells, can modulate the nuclear polyphosphoinositide signalling pathway of the osteoblasts involved in bone remodelling.
authors.affiliation: Sezioni di Bologna c/o IOR, ITOI-CNR, via di Barbiano 1/10, 40136 Bologna, Italy.
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Zini
authors.forename: Gina
authors.initials: G
authors.lastname: Lisignoli
authors.forename: Liliana
authors.initials: L
authors.lastname: Solimando
authors.forename: Alberto
authors.initials: A
authors.lastname: Bavelloni
authors.forename: Francesco
authors.initials: F
authors.lastname: Grassi
authors.forename: Lia
authors.initials: L
authors.lastname: Guidotti
authors.forename: Carmela
authors.initials: C
authors.lastname: Trimarchi
authors.forename: Andrea
authors.initials: A
authors.lastname: Facchini
authors.forename: Nadir Mario
authors.initials: NM
authors.lastname: Maraldi
mesh_terms: D001172:Arthritis, Rheumatoid / Q000201:enzymology*; D002467:Cell Nucleus / Q000201:enzymology; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007375:Interleukin-1 / Q000494:pharmacology*; D007527:Isoenzymes / Q000378:metabolism; D007867:Leg Bones / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010003:Osteoarthritis / Q000201:enzymology; D010006:Osteoblasts / Q000201:enzymology*; D054799:Phospholipase C beta; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D010738:Type C Phospholipases / Q000378:metabolism
chemical_list: D007375:Interleukin-1; D007527:Isoenzymes; D009419:Nerve Tissue Proteins; D014409:Tumor Necrosis Factor-alpha; C097702:synaptojanin; D010744:Phosphoric Monoester Hydrolases; D010738:Type C Phospholipases; C518033:PLCB1 protein, human; D054799:Phospholipase C beta