A new method for the evaluation of antibody affinity based on serial dilutions of studied antibody-antigen mixture.
A new method for the evaluation of the affinity of bivalent antibodies were suggested. This method is based on the previously published by the author the idea of using so-called coordinates of dilutions. It was shown that the suggested method allows to evaluate the affinity of antibodies with high accuracy using this simple approach. It is supposed that at some conditions the suggested method could have substantial advantages in comparison to the traditional methods. This method allows to analyze situations when antibodies are already in a mixture with antigen, for example in the bloodstream in the case of infections or autoimmune diseases. The method provides useful approach for the evaluation not only antibody affinity, but also the concentration of circulated antigen.
Bobrovnik
S A
SA
Palladin Institute of Biochemistry, NAS of Ukraine, Kyiv. sab@biochem.kiev.ua
D000818:Animals; D018033:Antibodies, Bispecific / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D000915:Antibody Affinity; D000937:Antigen-Antibody Reactions; D002417:Cattle; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D008433:Mathematics; D010047:Ovalbumin / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000276:immunology
D018033:Antibodies, Bispecific; D000911:Antibodies, Monoclonal; D012710:Serum Albumin, Bovine; D010047:Ovalbumin