title: Imaging of striatal dopamine transporters in rat brain with single pinhole SPECT and co-aligned MRI is highly reproducible.
abstract: A recently developed pinhole high-resolution SPECT system was used to measure striatal to non-specific binding ratios in rats (n = 9), after injection of the dopamine transporter ligand (123)I-FP-CIT, and to assess its test/retest reproducibility. For co-alignment purposes, the rat brain was imaged on a 1.5 Tesla clinical MRI scanner using a specially developed surface coil. The SPECT images showed clear striatal uptake. On the MR images, cerebral and extra-cerebral structures could be easily delineated. The mean striatal to non-specific [(123)I]FP-CIT binding ratios of the test/retest studies were 1.7 +/- 0.2 and 1.6 +/- 0.2, respectively. The test/retest variability was approximately 9%. We conclude that the assessment of striatal [(123)I]FP-CIT binding ratios in rats is highly reproducible.
authors.affiliation: Department of Nuclear Medicine, University of Amsterdam, Academic Medical Center, Amsterdam, The Netherlands. J.Booij@amc.uva.nl
authors.forename: Jan
authors.initials: J
authors.lastname: Booij
authors.forename: Kora
authors.initials: K
authors.lastname: de Bruin
authors.forename: Maartje M L
authors.initials: MM
authors.lastname: de Win
authors.forename: Cristina
authors.initials: C
authors.lastname: Lavini
authors.forename: Gerard J
authors.initials: GJ
authors.lastname: den Heeten
authors.forename: Jan B A
authors.initials: JB
authors.lastname: Habraken
mesh_terms: D000818:Animals; D003342:Corpus Striatum / Q000000981:diagnostic imaging* / Q000378:metabolism*; D050483:Dopamine Plasma Membrane Transport Proteins; D019544:Equipment Failure Analysis; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins / Q000378:metabolism*; D009419:Nerve Tissue Proteins; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013382:Subtraction Technique; D014018:Tissue Distribution; D015899:Tomography, Emission-Computed, Single-Photon / Q000295:instrumentation* / Q000379:methods*
chemical_list: D050483:Dopamine Plasma Membrane Transport Proteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D019275:Radiopharmaceuticals