title: The role of nucleosome positioning in repression by the yeast alpha 2/Mcm1p repressor.
abstract: In Saccharomyces cerevisiae, alpha 2/Mcm1p represses a-cell specific genes. Previous studies indicated that repression by alpha 2/Mcm1p is associated with organization of positioned nucleosomes in promoters of a-cell specific genes such as STE6 and BAR1. Here, we examined the role of nucleosome positioning in repression of STE6-lacZ by alpha 2/Mcm1p. We showed that insertion of an A34 sequence, which is shown to disrupt an array of positioned nucleosomes, affects STE6-lacZ expression. Furthermore, artificial recruitment of TBP is facilitated by the insertion of A34 to derepress STE6-lacZ expression. These findings support the model that nucleosome positioning plays a regulatory role in repression by alpha 2/Mcm1p.
authors.affiliation: Department of Chemistry, Meisei University, Hino, Tokyo 191-8506, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Saito
authors.forename: Shigeki
authors.initials: S
authors.lastname: Miura
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Heisaburo
authors.initials: H
authors.lastname: Shindo
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Shimizu
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D006023:Glycoproteins; D007763:Lac Operon; D026341:Minichromosome Maintenance 1 Protein / Q000502:physiology*; D009707:Nucleosomes / Q000378:metabolism*; D012097:Repressor Proteins / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D018528:ATP-Binding Cassette Transporters; D005656:Fungal Proteins; D006023:Glycoproteins; D026341:Minichromosome Maintenance 1 Protein; D009707:Nucleosomes; D012097:Repressor Proteins; C489277:STE6 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins