[The variation of Caspase3 activity in tanshinone induced NB4 cells apoptosis].
OBJECTIVE
To study the variation and role of Caspase3 activity in the process of Tanshinone (Tan II A) induced NB4 cells apoptosis.


METHODS
NB4 cell apoptosis induced by Tan II A was demonstrated by cell morphology, DNA content analysis and DNA fragmentation assay. Caspase3 activity was determined by spectrofluorometry, and its inhibitory assay was performed using N-acetyl-Asp-Glu-Val-Asp-aldehyde(AC-DEVD-CHO).


RESULTS
Tan II A could induce NB4 cell apoptosis accompanied with increase of caspase3 activity. The induction of NB4 cell apoptosis by use of Tan II A could be partially inhibited by AC-DEVD-CHO.


CONCLUSIONS
The induction of NB4 cell apoptosis by Tan II A could be fulfilled by activating Caspase3.
Liang
Xiaogong
X
Department of Hematology, West China Hospital, Sichuan University, Chengdu 610041, China.
Yang
Yiming
Y
Deng
Chengqi
C
Meng
Wentong
W
Liu
Ting
T
Jia
Yongqian
Y
Cheng
Yunfeng
Y
Li
Li
L
D045784:Abietanes; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism*; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000188:drug therapy* / Q000473:pathology; D010616:Phenanthrenes / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D045784:Abietanes; D000972:Antineoplastic Agents, Phytogenic; D010616:Phenanthrenes; C021751:tanshinone; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases