A simple and rapid method to determine the zygosity of uPA-transgenic SCID mice.
Successful transplantation of xenogeneic hepatocytes into uPA-transgenic SCID mice depends on the zygosity of the recipient mice. Normally, the difference between homozygous and heterozygous animals is determined via a quantitative Southern blot. We sequenced a part of the mouse genome that is eliminated upon integration of the transgene in the genome. Based on that sequence we developed a multiplex PCR that allows the unambiguous discrimination of negative, heterozygous, and homozygous uPA-transgenic SCID mice in a single day procedure. The speed of the procedure is an essential quality because transplantation of xenogeneic hepatocytes into uPA-SCID mice should be done as soon as possible after birth.
Meuleman
P
P
Center for Vaccinology, Ghent University, De Pintelaan 185, Ghent 9000, Belgium.
Vanlandschoot
P
P
Leroux-Roels
G
G
D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D004195:Disease Models, Animal; D006509:Hepatitis B / Q000209:etiology; D006526:Hepatitis C / Q000209:etiology; D022781:Hepatocytes / Q000637:transplantation; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D014183:Transplantation, Heterologous; D014568:Urokinase-Type Plasminogen Activator / Q000235:genetics*
D014568:Urokinase-Type Plasminogen Activator