title: Co-expression pattern of Shh with Prox1 and that of Nkx2.2 with Mash1 in mouse taste bud.
abstract: In mammals, taste buds are maintained by continuous turnover of cells, even in adulthood. Cell proliferation and differentiation continue to produce taste cells, which express various genes related to taste reception. We found the co-expression of Sonic hedgehog (Shh) with Prox1 and that of Nkx2.2 with Mash1 in adult mouse taste buds. Whereas Prox1was expressed strongly in cells in the basal region of mouse taste buds where Shh was co-expressed, it was expressed weakly in almost all taste bud cells lacking Shh expression. At 0.5 day after birth, when taste cells have not yet differentiated, the expressions of Shh and Prox1 completely overlapped in the epithelium of circumvallate papillae. Nkx2.2 was observed in cells expressing Mash1, but not in cells expressing genes related to taste reception, such as gustducin and T1R3. Almost all fusiform cells expressing Mash1 co-expressed Nkx2.2, while the majority of round cells expressing Mash1 in the basal region of taste buds lacked Nkx2.2 expression.
authors.affiliation: National Food Research Institute, 2-1-12 Kannondai, Ibaraki Tsukuba-shi 305-8642, Japan. hmiura@nfri.affrc.go.jp
authors.forename: Hirohito
authors.initials: H
authors.lastname: Miura
authors.forename: Yuko
authors.initials: Y
authors.lastname: Kusakabe
authors.forename: Hiromi
authors.initials: H
authors.lastname: Kato
authors.forename: Jun
authors.initials: J
authors.lastname: Miura-Ohnuma
authors.forename: Mizuho
authors.initials: M
authors.lastname: Tagami
authors.forename: Yuzo
authors.initials: Y
authors.lastname: Ninomiya
authors.forename: Akihiro
authors.initials: A
authors.lastname: Hino
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D053823:Hedgehog Proteins; D018257:Helix-Loop-Helix Motifs; D000090322:Homeobox Protein Nkx-2.2; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D017403:In Situ Hybridization; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D015347:RNA Probes; D013650:Taste Buds / Q000166:cytology / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D025521:Tumor Suppressor Proteins; D029961:Zebrafish Proteins; D000098551:Prospero-Related Homeobox 1 Protein
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D004268:DNA-Binding Proteins; D053823:Hedgehog Proteins; D000090322:Homeobox Protein Nkx-2.2; D018398:Homeodomain Proteins; D015347:RNA Probes; D015534:Trans-Activators; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; D029961:Zebrafish Proteins; D000098551:Prospero-Related Homeobox 1 Protein; C499317:Ascl1 protein, mouse; C000715627:Nkx2-2 protein, mouse; C465570:Shh protein, mouse; C000715630:nkx2.2b protein, zebrafish