title: Adenovirus type 11 uses CD46 as a cellular receptor.
abstract: The 51 human adenovirus serotypes are divided into six species (A to F). Many adenoviruses use the coxsackie-adenovirus receptor (CAR) for attachment to host cells in vitro. Species B adenoviruses do not compete with CAR-binding serotypes for binding to host cells, and it has been suggested that species B adenoviruses use a receptor other than CAR. Species B adenoviruses mainly cause disease in the respiratory tract, the eyes, and in the urinary tract. Here we demonstrate that adenovirus type 11 (Ad11; of species B) binds to Chinese hamster ovary (CHO) cells transfected with CD46 (membrane cofactor protein)-cDNA at least 10 times more strongly than to CHO cells transfected with cDNAs encoding CAR or CD55 (decay accelerating factor). Nonpermissive CHO cells were rendered permissive to Ad11 infection upon transfection with CD46-cDNA. Soluble Ad11 fiber knob but not Ad7 or Ad5 knob inhibited binding of Ad11 virions to CD46-transfected cells, and anti-CD46 antibodies inhibited both binding of and infection by Ad11. From these results we conclude that CD46 is a cellular receptor for Ad11.
authors.affiliation: Department of Virology, Umeå University, SE-90185 Umeå, Sweden.
authors.forename: Anna
authors.initials: A
authors.lastname: Segerman
authors.forename: John P
authors.initials: JP
authors.lastname: Atkinson
authors.forename: Marko
authors.initials: M
authors.lastname: Marttila
authors.forename: Veronica
authors.initials: V
authors.lastname: Dennerquist
authors.forename: Göran
authors.initials: G
authors.lastname: Wadell
authors.forename: Niklas
authors.initials: N
authors.lastname: Arnberg
mesh_terms: D000260:Adenoviruses, Human / Q000145:classification / Q000472:pathogenicity / Q000502:physiology*; D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000235:genetics / Q000502:physiology*; D001483:Base Sequence; D018958:CD55 Antigens / Q000235:genetics / Q000502:physiology; D016466:CHO Cells; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D006224:Cricetinae; D004274:DNA, Recombinant / Q000235:genetics; D006801:Humans; D008345:Manganese / Q000378:metabolism; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins / Q000235:genetics / Q000502:physiology*; D011991:Receptors, Virus / Q000235:genetics / Q000502:physiology*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D014357:Trypsin / Q000494:pharmacology
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; C497857:CD46 protein, human; D018958:CD55 Antigens; C568771:CLMP protein, human; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D004274:DNA, Recombinant; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins; D011991:Receptors, Virus; D011994:Recombinant Proteins; C085301:adenovirus receptor; D008345:Manganese; D014357:Trypsin; D002118:Calcium