[Analysis of lecithin-cholesterol acyltransferase cDNA and protein sequence from tree shrew].
OBJECTIVE
To acquire cDNA sequence of lecithin-cholesterol acyltransferase (LCAT) from tree shrew and analyze the sequence structure.


METHODS
The first strand cDNA was acquired by reverse transcription using mRNA from tree shrew liver as template. By the method of SMART RACE PCR, tree shrew LCAT cDNA was acquired and deduced its amino acids sequence. The sequence and structure of tree shrew LCAT cDNA and amino acid were analyzed and predicted by the molecular software.


RESULTS
Tree shrew LCAT cDNA is composed of 1,340 bp, including 2 bp 5' untranslated region (5' UTR), 1,320 bp open reading frame (ORF) which encodes protein precursor of 440 amino acids (24 amino acids signal peptide and 416 amino acids mature peptide), and 18 bp 3' untranslated region (3'UTR). The stop codon is TAA and there is a poly (A) signal sequence AATAAA and a 25 bp poly (A) tail. Tree shrew LCAT cDNA sequence has been accepted by GenBank as a new gene, accession number AF272861 and its homology with human and baboon was 90% and 89%, respectively.


CONCLUSIONS
The sequence of LCAT cDNA in tree shrew has high identity with that of human and other experimental animal species.
Zhang
Jian
J
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China.
Zeng
Wu-wei
WW
Chen
Bao-sheng
BS
Wu
Gang
G
Zhang
Wen-cheng
WC
Zhang
Ke-man
KM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D008099:Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000737:chemistry* / Q000235:genetics*; D020539:Sequence Analysis, Protein; D014418:Tupaiidae
D018076:DNA, Complementary; D007862:Phosphatidylcholine-Sterol O-Acyltransferase