[Arsenic trioxide induces human tumor cell apoptosis and G2 + M arrest whereas causes HPV16 DNA immortalized cervical epithelial cells G1 block].
OBJECTIVE
To investigate whether As2O3 induces tumor cell apoptosis and cell cycle arrest.


METHODS
DNA agarose gel electrophoresis, flowcytometry analysis, RT-PCR, and Western blot.


RESULTS
As2O3 induced G2 + M arrest of tumor cells such as GLC-82, MGC-803, SGC-7901, Eca109 and HeLa cells before inducing apoptosis and decreased c-myc gene expression, whereas caused G1 block of HPV16 DNA immortalized cervical epithelial HCE16/3 cells and did not affect c-myc expression.


CONCLUSIONS
As2O3 caused apoptosis was related with cell cycle arrest. However, the phase of arrest of tumor cells was different from the pre-tumor cell HCE16/3 cells, which could be related to the change of c-myc gene expression.
Lin
C
C
National Laboratory of Molecular Oncology, Cancer Institute, CAMS, PUMC, Beijing 100021, China.
Deng
Y
Y
Zheng
J
J
Fu
M
M
Chen
J P
JP
Xiao
P
P
Wu
M
M
D017209:Apoptosis / Q000187:drug effects*; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D002584:Cervix Uteri / Q000166:cytology*; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects*; D005260:Female; D016193:G1 Phase; D016195:G2 Phase; D005786:Gene Expression Regulation; D016259:Genes, myc / Q000235:genetics; D006801:Humans; D008297:Male; D010087:Oxides / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D001152:Arsenicals; D010087:Oxides; D000077237:Arsenic Trioxide