Inhibitory potential of Crotalus durissus terrificus venom on measles virus growth.
This paper presents the antiviral activity found in a snake with Crotalus durissus terrificus venom (Cdt), studied by use of microplate inhibition assay, using measles virus (MV). Cdt at concentrations below 100 microg/ml showed no cytotoxicity for Vero cells. This study shows the optimal conditions for cell treatment and infection. Two factors that affect virus binding and infection efficiency were studied: the use of an adsorption step, where infection volume was varied; and the concentration of fetal bovine serum (FBS). The adsorption step, with or without FBS, increased the bound virus percentage, whereas it increased bound virus at equilibrium only in FBS-free until 2.5% FBS. In contrast, the addition of 10% FBS decreased the bound virus percentage. The inhibition of MV replication in Vero cells was observed when Cdt was added either before or during cell infection with virus. Its inhibitory concentration against MV replication was 0.1 until 100 microg/ml, respectively. The anti-MV effect of the Cdt was gradually decreased when it was added before or during infection, and little inhibition was observed when Cdt was added 1 h after infection, suggesting that the MV infection was inhibited at the time of the initial events such as at the moment of adsorption and penetration of the viral cycle. In conclusion, Cdt contains anti-MV effects that may be of potential clinical interest.
Petricevich
Vera L
VL
Laboratório de Imunoquímica, Instituto Butantan, Av. Vital Brasil n. 1500, CEP 05504-900 São Paulo, Brazil. velupetri@hotmail.com
Mendonça
Ronaldo Z
RZ
D000818:Animals; D001667:Binding, Competitive; D002522:Chlorocebus aethiops; D003435:Crotalid Venoms / Q000494:pharmacology* / Q000633:toxicity; D017839:Crotalus; D008459:Measles virus / Q000187:drug effects* / Q000254:growth & development; D051379:Mice; D014709:Vero Cells; D038301:Virus Inactivation / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects*
D003435:Crotalid Venoms