A study on PCR for detecting infection with M. leprae.
OBJECTIVE
So far, it has not been established a satisfactory method for early diagnosis and studying on epidemiology for leprosy, we want to develop a molecular biological method for solving this point.


METHODS
Based on the M. leprae gene coding groEL, 65 kD and 16S rRNA, three polymerase chain reactions were developed by using Plikaytis', Woods' and Pattyn's procedures. It was optimized that the experimental parameters for each PCR, and a comparative study on practivity among three PCRs was also conducted for practical purpose.


CONCLUSIONS
For detecting infection with M. leprae, all of PCRs established by us were highly sensitive and specific, but for practical purpose, the Woods' PCR optimized by us ought to be chosen firstly.
Qinxue
W
W
Institute of Dermatology, CAMS & PUMC, Nanjing 210042.
Xinyu
L
L
Wei
H
H
Tao
L
L
Yaoping
Y
Y
Jinping
Z
Z
Xiuling
C
C
Ganyun
Y
Y
D018834:Chaperonin 60 / Q000235:genetics; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D007918:Leprosy / Q000175:diagnosis* / Q000235:genetics / Q000382:microbiology; D009166:Mycobacterium leprae / Q000235:genetics*; D009169:Mycobacterium tuberculosis / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012680:Sensitivity and Specificity
D018834:Chaperonin 60; D017931:DNA Primers; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S