Human rhabdomyosarcoma cells for rapid detection of enteroviruses by shell-vial assay.
The ability of the RD (rhabdomyosarcoma) and MRC-5 cell-lines to detect enteroviruses in 33 clinical samples (cerebrospinal fluid, stools and throat swabs) was evaluated. The samples had previously tested enterovirus-positive by traditional tube-culture and had been frozen after their initial processing. By traditional tube-culture, 100 and 85 % of samples were positive for enterovirus in RD and MRC-5 cells, respectively. By rapid shell-vial assay, 94 and 45.5 % were positive after 48 h incubation in RD and MRC-5 cells, respectively. RD cells supported growth of all enterovirus serotypes, whereas MRC-5 cells were not able to detect any of the three coxsackieviruses that were found (one coxsackievirus A9 and two coxsackievirus B5). The shell-vial assay with RD cell-lines may be a useful tool for rapid diagnosis of enteroviral infection.
Pérez-Ruiz
M
M
Servicio de Microbiología, Hospital Universitario Virgen de las Nieves, Avda Fuerzas Armadas s/n, 18014 Granada, Spain.
Navarro-Marí
J M
JM
Servicio de Microbiología, Hospital Universitario Virgen de las Nieves, Avda Fuerzas Armadas s/n, 18014 Granada, Spain.
Palacios Del Valle
E
E
Servicio de Microbiología, Hospital Universitario Virgen de las Nieves, Avda Fuerzas Armadas s/n, 18014 Granada, Spain.
Rosa-Fraile
M
M
Servicio de Microbiología, Hospital Universitario Virgen de las Nieves, Avda Fuerzas Armadas s/n, 18014 Granada, Spain.
D002555:Cerebrospinal Fluid / Q000821:virology; D004770:Enterovirus / Q000254:growth & development / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000175:diagnosis* / Q000821:virology; D005243:Feces / Q000821:virology; D006801:Humans; D010614:Pharynx / Q000821:virology; D012208:Rhabdomyosarcoma / Q000821:virology*; D013997:Time Factors; D014407:Tumor Cells, Cultured; D014776:Virus Cultivation / Q000295:instrumentation / Q000379:methods