Outbreak of extended-spectrum beta-lactamase VEB-1-producing isolates of Acinetobacter baumannii in a French hospital.
Twelve clonally related and multidrug-resistant Acinetobacter baumannii isolates were recovered during a 4-month period from 12 patients hospitalized at the Valenciennes Hospital in France. Antibiograms determined by the double-disk diffusion technique on cloxacillin-containing plates detected a clavulanic acid-inhibited extended-spectrum beta-lactamase (ESBL). PCR and sequencing identified the gene encoding the Ambler class A ESBL VEB-1. This gene was located on the chromosome and was part of a class 1 integron identical to that previously identified in Pseudomonas aeruginosa isolates from Thailand. Additionally, seven clonally related bla(VEB-1)-positive A. baumannii strains were identified in the immediate environment of the hospitalized patients. This is the first report of the ESBL VEB-1 in Acinetobacter spp. and the first description of VEB-1-producing strains as a source of an outbreak occurring outside Southeast Asia. This report underlines the difficulty of the identification of ESBLs in A. baumannii.
Poirel
Laurent
L
Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 94275 Le Kremlin-Bicêtre, France.
Menuteau
Olivier
O
Agoli
Nathalie
N
Cattoen
Christian
C
Nordmann
Patrice
P
D000151:Acinetobacter Infections / Q000175:diagnosis* / Q000453:epidemiology; D040981:Acinetobacter baumannii / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D003428:Cross Infection / Q000175:diagnosis* / Q000453:epidemiology; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D029968:Escherichia coli Proteins; D005602:France / Q000453:epidemiology; D005798:Genes, Bacterial; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods; D015183:Restriction Mapping; D012189:Retrospective Studies; D001618:beta-Lactamases / Q000235:genetics*
D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C119424:beta-lactamase VEB-1, E coli; D001618:beta-Lactamases