[Effect of AP-1 on expression of plasminogen activator inhibitor-1 in human vascular endothelial cells].
OBJECTIVE
To study the effect of nuclear transcription factor AP-1 on tumor necrosis factor alpha (TNF-alpha) or minimal modified low density lipoprotein (mmLDL)-induced expression of plasminogen activator inhibitor-1 (PAI-1) in human vascular endothelial cells.


METHODS
Using gene recombination techniques, four luciferase reporter gene plasmids containing different length of human PAI-1 gene promoter were constructed. Through the transient transfection analysis, the roles of AP-1 element (from -823 bp to -553 bp) in PAI-1 promoter have been determined. In order to further verify the role of AP-1 element, the three site-directed mutants were recovered using PCR and sequencing assay.


RESULTS
The induction by TNF-alpha or mmLDL were decreased markedly when the three AP-1 elements in PAI-1 promoter had been mutated respectively.


CONCLUSIONS
These results indicate that the AP-1 element in PAI-1 promoter may have important role in PAI-1 gene transcriptions in endothelial cells induced by TNF-alpha or mmLDL.
Li
Xiao-dong
XD
Department of Pathophysiology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China.
Zu
Shu-yu
SY
Wang
Wen
W
Zhu
Guang-jin
GJ
D001483:Base Sequence; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D017395:Plasminogen Activator Inhibitor 1 / Q000096:biosynthesis* / Q000235:genetics; D011401:Promoter Regions, Genetic; D018808:Transcription Factor AP-1 / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D014471:Umbilical Veins / Q000166:cytology
D017395:Plasminogen Activator Inhibitor 1; D018808:Transcription Factor AP-1; D014409:Tumor Necrosis Factor-alpha