DNA damage induced by polyglutamine-expanded proteins.
We have developed stable cell lines expressing green fluorescent protein fusion proteins containing polyglutamine repeats of various lengths under tetracycline control. The expression of the expanded (43Q) repeat protein resulted in aggregate formation in a time-dependent fashion. The accumulation of aggregates did not induce apoptosis, although the survival of these cells was critically dependent on the presence of serum and growth factors. However, the expression of 43Q expanded protein strongly activated the ataxia telangiectasia mutated kinase/ATM and Rad3-related kinase (ATM/ATR)-dependent DNA damage response, as shown by selective phosphorylation of ATM substrates. This activation was dependent on 43 CAG protein expression, reversible and sensitive to caffeine and reducing agents. Similarly, we found phosphorylated ATM substrates in fibroblasts from Huntington's disease or SCA-2 patients. Oxidative stress induced accumulation of ATM/ATR phosphorylated protein in HD and SCA-2 patients, but not in normal controls. Furthermore, a significant phosphorylation of H2AX was shown by fibroblasts from patients. We conclude that polyglutamine induces ATM/ATR-dependent DNA damage response through accumulation of reactive oxygen species. ATM activation can be used to monitor the disease in vivo.
Giuliano
Paola
P
BioGem Consortium, Università 'Federico II', via Pansini 5, 80131 Napoli, Italy.
De Cristofaro
Tiziana
T
Affaitati
Adelina
A
Pizzulo
Grazia M
GM
Feliciello
Antonio
A
Criscuolo
Chiara
C
De Michele
Giuseppe
G
Filla
Alessandro
A
Avvedimento
Enrico V
EV
Varrone
Stelio
S
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D064007:Ataxia Telangiectasia Mutated Proteins; D002110:Caffeine / Q000494:pharmacology; D018797:Cell Cycle Proteins; D002460:Cell Line; D002478:Cells, Cultured; D004249:DNA Damage; D004268:DNA-Binding Proteins; D004789:Enzyme Activation; D005347:Fibroblasts / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D049452:Green Fluorescent Proteins; D006657:Histones / Q000378:metabolism; D006816:Huntington Disease / Q000473:pathology; D008164:Luminescent Proteins / Q000378:metabolism; D016716:PC12 Cells; D010455:Peptides / Q000235:genetics; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D051381:Rats; D017382:Reactive Oxygen Species / Q000032:analysis / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D020754:Spinocerebellar Ataxias / Q000473:pathology; D013312:Stress, Physiological / Q000503:physiopathology; D013379:Substrate Specificity; D013997:Time Factors; D019680:Trinucleotide Repeat Expansion / Q000235:genetics; D025521:Tumor Suppressor Proteins
D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D006657:Histones; D008164:Luminescent Proteins; D010455:Peptides; D017382:Reactive Oxygen Species; D011993:Recombinant Fusion Proteins; D025521:Tumor Suppressor Proteins; D049452:Green Fluorescent Proteins; C097188:polyglutamine; D002110:Caffeine; D011494:Protein Kinases; D064007:Ataxia Telangiectasia Mutated Proteins; D017346:Protein Serine-Threonine Kinases