Methamphetamine induces oligodendroglial cell death in vitro.
We investigated whether the psychostimulant methamphetamine (METH) has a cytotoxic effect on oligodendrocytes and which cell-death pathways are involved in the cytotoxic process. METH caused concentration- and time-dependent cytotoxicity in rat oligodendrocyte cultures. METH induced apoptotic cell death and mRNA expression of pro-apoptotic proteins (bax and DP5), but not anti-apoptotic proteins (bcl-2 and bcl-XL). These results suggest that METH induces cytotoxicity in rat oligodendrocytes via the differential regulation of the expression of genes involved in the apoptotic process.
Genc
Kursad
K
Department of Physiology, Dokuz Eylul University, Inciralti, 35340, Izmir, Turkey. kkursadgenc@hotmail.com
Genc
Sermin
S
Kizildag
Sefa
S
Sonmez
Ulker
U
Yilmaz
Osman
O
Tugyan
Kazim
K
Ergur
Bekir
B
Sonmez
Atac
A
Buldan
Zisan
Z
D000818:Animals; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins; D003603:Cytotoxins / Q000494:pharmacology*; D008694:Methamphetamine / Q000494:pharmacology*; D009479:Neuropeptides / Q000235:genetics; D009836:Oligodendroglia / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology*; D011518:Proto-Oncogene Proteins / Q000235:genetics; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein
D051017:Apoptosis Regulatory Proteins; C494828:Bax protein, rat; C494813:Bcl2l1 protein, rat; D003603:Cytotoxins; C484585:Hrk protein, rat; D009479:Neuropeptides; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein; D008694:Methamphetamine