Fluorescence microscopic visualization of a DNA-cationic fullerene complex.
Cationic fullerene derivative 1, which is soluble in aqueous medium, bound to double stranded DNA. Observation of Coliphage T4 DNA in the absence and presence of 1 by fluorescence microscopy suggested that 1 bound to DNA along its groove. Upon irradiation of visible light on the DNA complex of 1, DNA was cleaved dramatically. The process of DNA photo-cleavage could be monitored continuously by fluorescence microscopy.
Yamashita
K
K
Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka 812-8581, Japan.
Iwataki
T
T
Hatta
T
T
Yoshikawa
K
K
Tsuge
O
O
Takagi
M
M
Takenaka
S
S
D017122:Bacteriophage T4 / Q000737:chemistry; D001665:Binding Sites; D002412:Cations; D004247:DNA / Q000737:chemistry* / Q000528:radiation effects / Q000648:ultrastructure*; D004279:DNA, Viral / Q000737:chemistry / Q000528:radiation effects / Q000648:ultrastructure; D037741:Fullerenes / Q000737:chemistry* / Q000528:radiation effects; D066298:In Vitro Techniques; D046911:Macromolecular Substances; D008856:Microscopy, Fluorescence; D008958:Models, Molecular; D008968:Molecular Conformation; D009690:Nucleic Acid Conformation; D010777:Photochemistry
D002412:Cations; D004279:DNA, Viral; D037741:Fullerenes; D046911:Macromolecular Substances; D004247:DNA