Identification of glucose transporter-1 and it's functional assay in mouse glomerular mesangial cells cultured in vitro.
OBJECTIVE
To evaluate the role of glucose transporter-1 (GLUT1) in the glucose uptake of glomerular mesangial cells.


METHODS
Cultured C57/SJL mouse mesangial cells were used in the study. The expression of GLUT1 mRNA was detected by RT-PCR. The expression of GLUT1 protein was detected by immunofluorescence and flow cytometry. The uptake of glucose and its kinetics were determined by 2-deoxy-[3H] -D-glucose uptake.


RESULTS
Both GLUT1 mRNA and protein were found in mouse glomerular mesangial cells. 2-deoxy-D-glucose uptake and kinetics assay showed that this glucose transporter had high affinity for glucose and the glucose uptake specificity was further confirmed by phloretin.


CONCLUSIONS
Functional GLUT1 did present in mouse mesangial cells cultured in vitro and it might be the predominant transporter mediated the uptake of glucose into mesangial cells.
Zhang
J
J
Research Institute of Nephrology, Jingling Hospital, Nanjing University School of Medicine, Nanjing 210002.
Liu
Z
Z
Liu
D
D
Li
L
L
D000818:Animals; D002478:Cells, Cultured; D003847:Deoxyglucose / Q000378:metabolism*; D003928:Diabetic Nephropathies / Q000209:etiology; D005920:Glomerular Mesangium / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D051272:Glucose Transporter Type 1; D051379:Mice; D008810:Mice, Inbred C57BL; D009004:Monosaccharide Transport Proteins / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis
D051272:Glucose Transporter Type 1; D009004:Monosaccharide Transport Proteins; D012333:RNA, Messenger; C495678:Slc2a1 protein, mouse; D003847:Deoxyglucose