Cloning and characterization of Taenia saginata paramyosin cDNA.
A lambdaZAP-express cDNA library of Taenia saginata metacestodes was constructed. Antibody screening yielded a clone with an insert of 3,408 bp, an open reading frame of 2,589 bp, a deduced sequence of 863 amino acid and a molecular mass of 98.89 kDa. Alignments of the predicted amino acid sequence showed identity with paramyosins from several species: 98.8% with Taenia solium, 96.3% with Echinococcus.granulosus and about 70% with Schistosoma spp. The insert was expressed and purified. A collagen binding assay was performed which showed that T. saginata GST-paramyosin retained this property in a dose-dependent manner. Problems were encountered due to high backgrounds in serological assays in the homologous T. saginata system. However, the recombinant paramyosin was recognized by antibodies present in 31.6% of sera from T. solium seropositive cysticercosis patients and 100% of the sera from acute cysticercosis patients. The immunodominant epitope was the carboxyl-terminal fragment of the molecule.
Ferrer
Elizabeth
E
Instituto de Salud Carlos III, Centro Nacional de Microbiología, 28220 Majadahonda, Madrid, Spain.
Moyano
Eva
E
Benitez
Laura
L
González
Luis Miguel
LM
Bryce
Denise
D
Foster-Cuevas
Mildred
M
Dávila
Iris
I
Cortéz
Maria Milagros
MM
Harrison
Leslie J S
LJ
Parkhouse
R Michael E
RM
Gárate
Teresa
T
D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000469:parasitology; D003001:Cloning, Molecular; D003094:Collagen / Q000378:metabolism; D003551:Cysticercosis / Q000276:immunology / Q000469:parasitology; D018076:DNA, Complementary / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D011817:Rabbits; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013552:Swine; D041202:Taenia saginata / Q000235:genetics / Q000378:metabolism*; D014335:Tropomyosin / Q000737:chemistry* / Q000235:genetics / Q000276:immunology
D000909:Antibodies, Helminth; D018076:DNA, Complementary; D014335:Tropomyosin; D003094:Collagen