Expression of rhBMP-7 gene in transduced bone marrow derived stromal cells.
OBJECTIVE
To explore the possibility of expression of exogenous gene in transduced bone marrow derived stromal cells (BMSCs).


METHODS
The marker gene, pbLacZ, was transferred into cultured BMSCs and the expression of transduced gene by X-gal staining was examined. Then plasmid pcDNA3-rhBMP7 was delivered to cultured BMSCs. Through immunohistochemical staining and RT-PCR assay, the expression of rhBMP7 gene was detected.


RESULTS
The exogenous gene could be expressed efficiently in transduced BMSCs.


CONCLUSIONS
The present study provided a theoretical basis to gene therapy on the problems of bone and cartilage tissue.
Duan
Deyu
D
Department of Orthopaedics, Union Hospital, Tongji Medical University, Wuhan 430022.
Du
Jingyuan
J
Wang
Hong
H
Liu
Yong
Y
Guo
Xiaodong
X
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins / Q000096:biosynthesis* / Q000235:genetics; D002478:Cells, Cultured; D005822:Genetic Vectors / Q000235:genetics; D011817:Rabbits; D011994:Recombinant Proteins / Q000096:biosynthesis; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism*; D013977:Tibia / Q000166:cytology; D014162:Transfection; D016212:Transforming Growth Factor beta
D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta