In situ RNA-RNA hybridisation of phospholipase C beta 3 shows lack of expression in neuroendocrine tumours.
BACKGROUND
Phospholipase C beta 3 (PLCB3) plays an important role in the signal transduction of the seven transmembrane receptors. The gene is located in the vicinity of the Multiple Endocrine Neoplasia type 1 (MEN1) gene on chromosome 11q13. Transfection of PLCB3 to neuroendocrine cell lines lacking expression suppresses the neoplastic phenotype and affects the gene expression of S100A3 and human mismatch repair protein, suggesting a role for PLCB3 in neuroendocrine tumorigenesis.


METHODS
We used RNA-RNA in situ hybridisation for PLCB3 on a total of 82 samples including 34 from MEN1 patients.


RESULTS
We show that the PLCB3 transcript is missing in 8 out of 14 MEN1-associated neoplasias as well as in 4 out of 10 bronchial carcinoids, 2 out of 10 exocrine pancreatic cancers and one sporadic adrenocortical carcinoma.


CONCLUSIONS
Low or lack of PLCB3 expression in a subset of endocrine tumours, together with earlier published in vitro data on suppressor characteristics upon transfection, indicate that PLCB3 could be involved in the tumorigenesis in a subset of endocrine tumours.
Stålberg
Peter
P
Department of Surgical Sciences, University Hospital, S-751 85 Uppsala, Sweden. Peter.Stalberg@surgsci.uu.se
Granberg
Dan
D
Carling
Tobias
T
Wilander
Erik
E
Eriksson
Barbro
B
Gobl
Anders
A
Akerström
Goran
G
Rastad
Jonas
J
Modlin
Irvin M
IM
Oberg
Kjell
K
Skogseid
Britt
B
D000818:Animals; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics*; D018761:Multiple Endocrine Neoplasia Type 1 / Q000201:enzymology* / Q000235:genetics; D009115:Muridae; D018358:Neuroendocrine Tumors / Q000201:enzymology* / Q000235:genetics; D054799:Phospholipase C beta; D012334:RNA, Neoplasm / Q000096:biosynthesis* / Q000235:genetics; D010738:Type C Phospholipases / Q000096:biosynthesis / Q000235:genetics*
D007527:Isoenzymes; D012334:RNA, Neoplasm; D010738:Type C Phospholipases; C518039:PLCB3 protein, human; D054799:Phospholipase C beta