Oscillation of human period 1 (hPER1) reporter gene activity in human neuroblastoma cells in vivo.
The mammalian period (Per) genes, which are components of the circadian clock, are mainly regulated via an autoregulatory feedback loop. Here we provide evidence that human Per1 (hPER1) reporter gene activity shows circadian rhythmicity in a human neuroblastoma, but not in a astrocytoma or a hepatoma cell line. Medium change and various pharmacological stimuli differentially induce this behavior. This circadian oscillation was strongly dampened and could be followed over maximally three cycles. It was even possible to phase-shift the course of this oscillation by repeated application of stimuli.
Maronde
Erik
E
IPF Pharmaceuticals GmbH, Hannover, Germany. erik.maronde@ipf-pharma.de
Motzkus
Dirk
D
D018797:Cell Cycle Proteins; D045744:Cell Line, Tumor; D002940:Circadian Rhythm / Q000235:genetics*; D005576:Colforsin / Q000494:pharmacology; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D017930:Genes, Reporter / Q000235:genetics; D006801:Humans; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009447:Neuroblastoma / Q000235:genetics*; D009687:Nuclear Proteins / Q000235:genetics*; D056950:Period Circadian Proteins; D010703:Phorbol Esters / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects; D011493:Protein Kinase C / Q000378:metabolism; D013997:Time Factors
D018797:Cell Cycle Proteins; D017362:Cyclic AMP Response Element-Binding Protein; D009687:Nuclear Proteins; C539022:PER1 protein, human; D056950:Period Circadian Proteins; D010703:Phorbol Esters; D005576:Colforsin; C032974:12-O-retinoylphorbol-13-acetate; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C; D020928:Mitogen-Activated Protein Kinases