Evaluation of [18F]FHPG as PET tracer for HSVtk gene expression.
In rats, the relationship between [(18)F]FHPG accumulation and HSVtk expression was studied with PET and autoradiography. [(18)F]FHPG distribution closely corresponded with HSVtk immunohistochemical staining. ROI analysis of tracer uptake 2 hours p.i. and Patlak graphical analysis were applied to quantify the PET data. For both analysis methods, the [(18)F]FHPG PET signal correlated well with the fraction of HSVtk expressing cells implanted, but showed a plateau when plotted against HSVtk protein levels. This might be due to rate limiting [(18)F]FHPG membrane transport.
de Vries
Erik F J
EF
PET Center, Groningen University Hospital, P.O. Box 30.001, 9700 RB Groningen, The Netherlands. e.f.j.de.vries@pet.azg.nl
van Dillen
Ingrid J
IJ
van Waarde
Aren
A
Willemsen
Antoon T M
AT
Vaalburg
Willem
W
Mulder
Nanno H
NH
Hospers
Geke A P
GA
D000818:Animals; D015774:Ganciclovir / Q000031:analogs & derivatives* / Q000493:pharmacokinetics*; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000000981:diagnostic imaging* / Q000235:genetics / Q000378:metabolism*; D008657:Metabolic Clearance Rate; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D051381:Rats; D011923:Rats, Nude; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013223:Statistics as Topic; D013937:Thymidine Kinase / Q000235:genetics / Q000378:metabolism*; D014055:Tomography, Emission-Computed / Q000379:methods*; D014915:Whole-Body Counting
C104325:9-((3-fluoro-1-hydroxy-2-propoxy)methyl)guanine; D019275:Radiopharmaceuticals; D013937:Thymidine Kinase; D015774:Ganciclovir