Regional differences of type II collagen synthesis in the human temporomandibular joint disc: immunolocalization study of carboxy-terminal type II procollagen peptide (chondrocalcin).
The purpose of this study was to determine the regional differences of distribution of the carboxy-terminal type II procollagen peptide (pCOL-II-C; chondrocalcin) as markers of cartilaginous expression in the human temporomandibular joint (TMJ) disc. Twelve human TMJ discs without morphologic abnormalities were obtained from 12 fresh cadavers. All specimens were analysed for pCOL-II-C expression using polyclonal rabbit anti-human pCOL-II-C antibody in avidin-biotin-peroxidase complex staining. The results were demonstrated that the percentage of pCOL-II-C immunoreactive disc cells was significantly higher in the outer part (the articular surfaces) than in the inner part (the deep central areas) of the disc. These findings suggest that the tissue heterogeneity of cartilaginous expression reflects the functional demands of the remodelling process in the human TMJ disc.
Kondoh
Toshirou
T
First Department of Oral and Maxillofacial Surgery, School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi Tsurumi-ku, Yokokama 230-8501, Japan. kondo-t@tsurumi-u.ac.jp
Hamada
Yoshiki
Y
Iino
Mitsuyoshi
M
Takahashi
Tetsu
T
Kikuchi
Toshiyuki
T
Fujikawa
Kyousuke
K
Seto
Kannichi
K
D000368:Aged; D000369:Aged, 80 and over; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D019902:Chondrocytes / Q000378:metabolism; D003094:Collagen / Q000378:metabolism*; D024043:Collagen Type II / Q000096:biosynthesis*; D006801:Humans; D007124:Immunoenzyme Techniques; D008875:Middle Aged; D019224:Temporomandibular Joint Disc / Q000033:anatomy & histology / Q000378:metabolism*
D002135:Calcium-Binding Proteins; D024043:Collagen Type II; C040072:chondrocalcin; D003094:Collagen