Synthesis and application of bis (tert-butyl)-1-methyl pyrenyl-silyl chloride as a 5'-hydroxyl protecting group for nucleosides and as a fluorescent purification handle in oligonucleotide synthesis.
A fluorescently labelled monomer viz. 5'-O-1, 1-bis-(tert-butyl)-1'-methyl-pyrenyl-silyl-thymidine-3'-phosphoramidite was synthesized. Different sequences were synthesized and were labelled using it and their fluorescence studies were carried out.
Tripathi
Snehlata
S
Nucleic Acids Research Laboratory, Department of Chemistry, University of Allahabad, Allahabad-211002, India.
Misra
Krishna
K
Sanghvi
Yogesh S
YS
D001483:Base Sequence; D005456:Fluorescent Dyes / Q000737:chemistry*; D009705:Nucleosides / Q000737:chemistry*; D009841:Oligonucleotides / Q000138:chemical synthesis*; D011721:Pyrenes / Q000737:chemistry*; D013936:Thymidine / Q000031:analogs & derivatives / Q000737:chemistry*
C476817:5'-O-1,1-bis(tert-butyl)-1'-methylpyrenylsilylthymidine-3'-phosphoramidite; D005456:Fluorescent Dyes; D009705:Nucleosides; D009841:Oligonucleotides; D011721:Pyrenes; D013936:Thymidine