title: A conserved domain in axonal targeting of Kv1 (Shaker) voltage-gated potassium channels.
abstract: Axonal voltage-gated potassium (Kv1) channels regulate action-potential invasion and hence transmitter release. Although evolutionarily conserved, what mediates their axonal targeting is not known. We found that Kv1 axonal targeting required its T1 tetramerization domain. When fused to unpolarized CD4 or dendritic transferrin receptor, T1 promoted their axonal surface expression. Moreover, T1 mutations eliminating Kvbeta association compromised axonal targeting, but not surface expression, of CD4-T1 fusion proteins. Thus, proper association of Kvbeta with the Kv1 T1 domain is essential for axonal targeting.
authors.affiliation: Howard Hughes Medical Institute, Departments of Physiology and Biochemistry, University of California, San Francisco, CA 94143-0725, USA.
authors.forename: Chen
authors.initials: C
authors.lastname: Gu
authors.forename: Yuh Nung
authors.initials: YN
authors.lastname: Jan
authors.forename: Lily Yeh
authors.initials: LY
authors.lastname: Jan
mesh_terms: D020816:Amino Acid Motifs; D019943:Amino Acid Substitution; D000818:Animals; D001369:Axons / Q000378:metabolism*; D001704:Biopolymers; D015704:CD4 Antigens / Q000378:metabolism; D019556:COS Cells; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D016764:Cell Polarity; D002478:Cells, Cultured; D003712:Dendrites / Q000378:metabolism; D004705:Endocytosis; D006624:Hippocampus / Q000166:cytology; D006801:Humans; D051663:Kv1.2 Potassium Channel; D008958:Models, Molecular; D016296:Mutagenesis; D009474:Neurons / Q000378:metabolism; D015221:Potassium Channels / Q000737:chemistry* / Q000378:metabolism*; D024642:Potassium Channels, Voltage-Gated; D017434:Protein Structure, Tertiary; D011990:Receptors, Transferrin / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D051637:Shaker Superfamily of Potassium Channels; D051672:Shal Potassium Channels; D014162:Transfection
chemical_list: D001704:Biopolymers; D015704:CD4 Antigens; C496969:KCNA2 protein, human; C498948:KCNAB2 protein, human; D051663:Kv1.2 Potassium Channel; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D011990:Receptors, Transferrin; D011993:Recombinant Fusion Proteins; D051637:Shaker Superfamily of Potassium Channels; D051672:Shal Potassium Channels