[Distribution of the labeled lipoproteins in the myocardium during perfusion of the isolated rat heart and adrenaline administration].
The distribution of different classes of colloidal gold-labeled lipoproteins (LP) was studied at the ultrastructural level in rat myocardium using the isolated heart perfusion following the adrenaline stimulation. The distribution of label was examined in endotheliocytes, macrophages and in the interstitial space. The addition of adrenaline to Krebs solution during 30 min of heart perfusion in vitro was shown to increase the amount, but to decrease the velocity of penetration of labeled LP of very low and low density into the myocardium. The intensive utilization of lipid component from LP in the conditions of stimulation provides for the enhanced metabolic requirements of the cardiomyocytes and good preservation of their ultrastructure. It is possible that colloidal gold-associated protein component of LP is incorporated by the endothelium more slowly. The label penetrates into the interstitium and is accumulated in the activated macrophages. If high density LP are added together with adrenaline into the perfusion medium the label does not leave the capillary wall, while macrophages and cardiomyocytes acquire the ultrastructural features of high functional activity.
Maksimov
V F
VF
Institute of Biochemistry, Siberian Branch of Russian Academy of Medical Sciences, Novosibirsk.
Kolpakov
A R
AR
Panin
L E
LE
Korostyshevskaia
I M
IM
Shalbueva
N I
NI
D000818:Animals; D004837:Epinephrine / Q000494:pharmacology*; D017979:Gold Colloid; D066298:In Vitro Techniques; D008074:Lipoproteins / Q000378:metabolism*; D008075:Lipoproteins, HDL / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism; D008079:Lipoproteins, VLDL / Q000378:metabolism; D008854:Microscopy, Electron; D009206:Myocardium / Q000378:metabolism* / Q000648:ultrastructure; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D013194:Staining and Labeling
D017979:Gold Colloid; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D004837:Epinephrine