Incorporation of nonnatural amino acids into proteins by using five-base codon-anticodon pairs.
A novel strategy for the incorporation of nonnatural amino acids into proteins was developed by using five-base codon-anticodon pairs. The streptavidin mRNA containing five-base codon CGGUA and the chemically aminoacylated tRNA with five-base anticodon UACCG were prepared, and added into E. coli in vitro translation system. As a result, the nonnatural amino acid was successfully incorporated into desired position of the protein. Other five-base codons CGGN1N2, where N1 and N2 indicate one of four nucleotides, were also available for the incorporation of the nonnatural amino acid.
Hohsaka
T
T
Department of Bioscience and Biotechnology, Okayama University, 3-1-1 Tsushimanaka, Okayama 700-8530, Japan.
Sisido
M
M
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry*; D000926:Anticodon / Q000235:genetics*; D001483:Base Sequence; D003062:Codon / Q000235:genetics*; D014176:Protein Biosynthesis; D015202:Protein Engineering; D011506:Proteins / Q000737:chemistry* / Q000235:genetics*; D012329:RNA, Bacterial / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D019809:Streptavidin / Q000031:analogs & derivatives / Q000737:chemistry / Q000235:genetics
D000596:Amino Acids; D000926:Anticodon; D003062:Codon; D011506:Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; D019809:Streptavidin