Stimulated microglia supernatants induced overexpression of neuronal nitric oxide gene in astrocytes.
The glia-glia, glia-neuron interactions are very complex and have not yet been clearly understood. Microglia gain reactivity in almost every type of brain insult and by affecting astrocytes and neurons, they determine the fate of damaged brain. In the present study we aimed to see the effect of stimulated microglia on nNOS and iNOS expression of astrocytes. The microglia cultures were stimulated with zymosanA and astrocytes were treated with the medium of microglia for 48 h. The results revealed that the astrocytes treated with microglia medium expressed more nNOS and iNOS than the ones treated with normal medium.
Esen
Nilufer
N
Department of Physiology, Medical Faculty, University of Kocaeli, Kocaeli, Turkey. nilufer@nilufer.com
Savli
Hakan
H
D000818:Animals; D001253:Astrocytes / Q000201:enzymology* / Q000502:physiology; D002474:Cell-Free System / Q000502:physiology; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D017628:Microglia / Q000166:cytology / Q000201:enzymology* / Q000502:physiology; D009474:Neurons / Q000201:enzymology / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000502:physiology*; D052248:Nitric Oxide Synthase Type I; D051381:Rats; D017207:Rats, Sprague-Dawley
D017077:Culture Media, Conditioned; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; C496322:Nos1 protein, rat