Stress protein expression in the Alzheimer-diseased choroid plexus.
Abnormal patterns of stress protein expression are found in the cerebral cortex and hippocampus of Alzheimer (AD) subjects. In this study, expression of various stress proteins in the Alzheimer-diseased choroid plexus (CP) was assessed immunohistochemically. We observed decreased HO-1 immunoreactivity in the AD CP, commensurate with our earlier report of suppressed HO-1 protein levels in AD cerebrospinal fluid (Schipper et al., Neurology 54:1297-1304, 2000). Heat shock protein (HSP) 90 was up-regulated in the AD CP relative to controls. There was a trend towards increased expression of HSP60, a mitochondrial stress protein; this is compatible with mitochondrial pathology recently documented in AD CP. Up-regulation of HSP90, a steroid receptor chaperone, in the AD CP may indicate abnormal hormone receptor expression in this secretory tissue. Glucose-regulated protein (GRP) 78 and 94 immunostaining was diminished in AD CP, implicating possible derangements in glucose or calcium homeostasis. Oxidative stress, per se, is probably not responsible for our observations because: i) there were no noticeable differences in the expression of HSP 70, ubiquitin, and alpha-B crystallin in the AD CP; and ii) augmentation, rather than the noted suppression, of HO-1 immunoreactivity would have been expected.
Anthony
Shawn G
SG
Division of Neuropathology, Department of Pathology, Rhode Island Hospital, Brown Medical School, Providence, RI 02903, USA.
Schipper
Hyman M
HM
Tavares
Rosemarie
R
Hovanesian
Virginia
V
Cortez
Selina C
SC
Stopa
Edward G
EG
Johanson
Conrad E
CE
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000201:enzymology / Q000378:metabolism* / Q000503:physiopathology*; D002352:Carrier Proteins / Q000378:metabolism*; D002831:Choroid Plexus / Q000503:physiopathology*; D003459:Crystallins / Q000378:metabolism*; D015536:Down-Regulation / Q000502:physiology; D000091342:Endoplasmic Reticulum Chaperone BiP; D005260:Female; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism*; D006360:Heat-Shock Proteins; D006419:Heme Oxygenase (Decyclizing) / Q000134:cerebrospinal fluid / Q000378:metabolism*; D051547:Heme Oxygenase-1; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008565:Membrane Proteins; D008875:Middle Aged; D018832:Molecular Chaperones / Q000378:metabolism*; D025801:Ubiquitin / Q000378:metabolism*; D015854:Up-Regulation / Q000502:physiology
D002352:Carrier Proteins; D003459:Crystallins; D000091342:Endoplasmic Reticulum Chaperone BiP; D018841:HSP90 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D008565:Membrane Proteins; D018832:Molecular Chaperones; D025801:Ubiquitin; C496541:HMOX1 protein, human; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1