title: Functional homology between yeast piD261/Bud32 and human PRPK: both phosphorylate p53 and PRPK partially complements piD261/Bud32 deficiency.
abstract: Yeast piD261/Bud32 belongs to the piD261 family of atypical protein kinases structurally conserved, from Archaea to human. The disruption of its gene is causative of severely defective growth. Its human homologue, PRPK, interacts with and phosphorylates the oncosuppressor p53 protein, which is lacking in yeast. Here we show that on one hand piD261/Bud32 interacts with and phosphorylates human p53 in vitro, on the other hand PRPK can partially complement the phenotype of yeast lacking the gene encoding piD261/Bud32. These data indicate that, despite considerable structural divergence, members of the piD261 family from distantly related organisms display a remarkable functional conservation.
authors.affiliation: Dipartimento di Chimica Biologica, Università di Padova, Viale G Colombo 3, 35121 Padova, Italy.
authors.forename: Sonia
authors.initials: S
authors.lastname: Facchin
authors.forename: Raffaele
authors.initials: R
authors.lastname: Lopreiato
authors.forename: Maria
authors.initials: M
authors.lastname: Ruzzene
authors.forename: Oriano
authors.initials: O
authors.lastname: Marin
authors.forename: Geppo
authors.initials: G
authors.lastname: Sartori
authors.forename: Claudia
authors.initials: C
authors.lastname: Götz
authors.forename: Mathias
authors.initials: M
authors.lastname: Montenarh
authors.forename: Giovanna
authors.initials: G
authors.lastname: Carignani
authors.forename: Lorenzo A
authors.initials: LA
authors.lastname: Pinna
mesh_terms: D017124:Conserved Sequence; D019143:Evolution, Molecular; D006801:Humans; D010766:Phosphorylation; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000502:physiology*; D040681:Structural Homology, Protein; D020349:Surface Plasmon Resonance; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D016159:Tumor Suppressor Protein p53; C108301:BUD32 protein, S cerevisiae; D017346:Protein Serine-Threonine Kinases