title: Mycobacterial interspersed repetitive unit typing of Mycobacterium tuberculosis compared to IS6110-based restriction fragment length polymorphism analysis for investigation of apparently clustered cases of tuberculosis.
abstract: An evaluation of the utility of IS6110-based restriction fragment length polymorphism (RFLP) typing compared to a combination of variable number tandem repeat (VNTR) typing and mycobacterial interspersed repetitive unit (MIRU) typing was undertaken. A total of 53 patient isolates of Mycobacterium tuberculosis from four presumed episodes of cross-infection were examined. Genomic DNA was extracted from the isolates by a cetyl trimethylammonium bromide method. The number of copies of tandem repeats of the five loci ETR(A) to ETR(E) and 12 MIRU loci was determined by PCR amplification and agarose gel electrophoresis of the amplicons. VNTR typing identified the major clusters of strains in the three investigations in which they occurred (each representing a different evolutionary clade: 32333, 42235, and 32433). The majority of unrelated isolates (by epidemiology and RFLP typing) were also identified by VNTR typing. The concordance between the RFLP and MIRU typing was complete, with the exception of two isolates with RFLP patterns that differed by one band each from the rest of the major epidemiologically linked groups of isolates in investigation A. All of these isolates had identical MIRU and VNTR types. A further pair of isolates differed in the number of tandem repeat copies at two MIRU alleles but had identical RFLP patterns. The speed of the combined VNTR and MIRU typing approach enabled results for some of the investigations to be supplied in "real time," influencing choices in contact tracing. The ease of comparison of results of MIRU and VNTR typing, which are recorded as single multidigit numbers, was also found to greatly facilitate investigation management and the communication of results to health care professionals.
authors.affiliation: Public Health Laboratory, Heartlands Hospital, Birmingham B9 5SS, United Kingdom. peter.hawkey@heartsol.wmids.nhs.uk
authors.forename: Peter M
authors.initials: PM
authors.lastname: Hawkey
authors.forename: E Grace
authors.initials: EG
authors.lastname: Smith
authors.forename: Jason T
authors.initials: JT
authors.lastname: Evans
authors.forename: Philip
authors.initials: P
authors.lastname: Monk
authors.forename: Gerry
authors.initials: G
authors.lastname: Bryan
authors.forename: Huda H
authors.initials: HH
authors.lastname: Mohamed
authors.forename: Madhu
authors.initials: M
authors.lastname: Bardhan
authors.forename: R Nicholas
authors.initials: RN
authors.lastname: Pugh
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D004196:Disease Outbreaks; D006801:Humans; D018598:Minisatellite Repeats; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid; D014376:Tuberculosis / Q000175:diagnosis* / Q000453:epidemiology
chemical_list: D004269:DNA, Bacterial