[Study on the relationship between angiogenesis and cervical lymphatic metastasis in head and neck squamous cell carcinomas].
OBJECTIVE
To investigate the relationship between tumor angiogenesis and cervical lymphatic metastasis and the expression of vascular endothelial growth factor in head and neck squamous cell carcinomas.


METHODS
Fifty-eight head and neck carcinoma tissue slides were immunohistochemically stained for anti-CD34 monoclonal antibody and anti-VEGF polyclonal antibody by SABC method. The intratumor microvessel density (IMVD) and the expression of vascular endothelial growth factor were measured.


RESULTS
1. The mean IMVD with cervical lymphatic metastasis was significantly higher than those without cervical lymphatic metastasis in head and neck squamous cell carcinomas(27.92 +/- 9.11 and 20.69 +/- 7.08, P < 0.01); 2. The expression of vascular endothelial growth factor had a positive correlation with IMVD in head and neck carcinoma tissues (rs = 0.487, P < 0.01).


CONCLUSIONS
IMVD may be an important predictor to cervical lymphatic metastasis in head and neck squamous cell carcinomas. Vascular endothelial growth factor may stimulate tumor angiogenesis in head and neck squamous cell carcinomas.
Zheng
Haizhou
H
Department of Otolaryngology, First Hospital, Medical College, Zhejiang University, Hangzhou 310003.
Lin
Shan
S
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000098:blood supply* / Q000556:secondary; D005260:Female; D006258:Head and Neck Neoplasms / Q000098:blood supply* / Q000473:pathology; D006801:Humans; D008198:Lymph Nodes / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009389:Neovascularization, Pathologic; D042442:Vascular Endothelial Growth Factors / Q000032:analysis
D042442:Vascular Endothelial Growth Factors