title: Vaccinia virus recombinant expressing an 87-kilodalton polyprotein that is sufficient to form astrovirus-like particles.
abstract: Human astrovirus is an important cause of acute gastroenteritis. We have generated, for the first time, a vaccinia virus recombinant expressing the astrovirus 87-kDa structural polyprotein. The results demonstrate that this expression results in the formation of virus-like particles in the absence of other astrovirus proteins and genomic RNA. The purified trypsin-activated virus-like particles strongly resemble the complete astrovirus particles.
authors.affiliation: Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Madrid, Spain.
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Dalton
authors.forename: Esperanza P
authors.initials: EP
authors.lastname: Pastrana
authors.forename: Alicia
authors.initials: A
authors.lastname: Sánchez-Fauquier
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D001257:Mamastrovirus / Q000502:physiology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D011995:Recombination, Genetic; D014616:Vaccinia virus / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology*
chemical_list: D017931:DNA Primers; D014764:Viral Proteins