[Morphofunctional changes in the midgut of tick females of the genus Ixodes (Acari: Ixodidae) during and after feeding].
Cyclic changes of the midgut epithelium were observed in females of 5 ticks species of the genus Ixodes during 7-10 days of feeding. The midgut epithelium of unfed females is represented by the digestive cells of nymphal phase and stem cells. The digestive cells of nymphal phase are functional during 1.5-2 days after attachment of the tick, and then, after the tearing away they go into the gut lumen. The secretory cells substitute the digestive cells of nymphal phase and finish their growth during the 4-4.5 days. Secretion of digestive enzymes is performed by the holocrine type with tearing away a whole cell. Intracellular digestion takes place in the digestive cells of four consequent generations. The secretory and digestive cells form a peritrophic matrix on their surface. The presence of peritrophic matrix gives an evidence the maturity and functional activity of the secretory and digestive cells. We suggest, that the peritrophic matrix takes part in intracellular digestion, namely in the process of micropinocytosis. The phagocytosis was not found in the ticks investigated. Digestion in the midgut lumen is performed by enzymes of the ruptured secretory and digestive cells, that is proved by the haemolysis of erythrocytes in the zone of their contact with these cells. The digestive cells of each generation functioned almost synchronously, with largest difference in starting about 12 hours.
Grigor'eva
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LA
D000818:Animals; D004064:Digestive System / Q000033:anatomy & histology / Q000378:metabolism; D004798:Enzymes / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology; D004848:Epithelium / Q000033:anatomy & histology / Q000378:metabolism; D005260:Female; D018884:Ixodes / Q000033:anatomy & histology* / Q000502:physiology*; D010873:Pinocytosis; D019518:Postprandial Period
D004798:Enzymes