title: Determination of urinary hippuric acid by micellar electrokinetic capillary chromatography.
abstract: We propose a method for the simultaneous determination of hippuric acid (HA) and creatinine based on capillary micellar electrokinetic chromatography. Experimental conditions were 20 mM sodium phosphate, pH 7.20, 25 mM sodium dodecyl sulfate, 5% (v/v) acetonitrile. Electropherograms evidenced HA and creatinine peaks in less than 12 min. The method showed good linearity for both analytes and satisfactory within-day precision. The present method, which is accurate, sensitive, rapid and simple, may be applied to single-spot urine samples.
authors.affiliation: Institute of Biochemistry and Clinical Biochemistry, Catholic University of Rome, Rome, Italy. czuppi@rm.unicatt.it
authors.forename: Cecilia
authors.initials: C
authors.lastname: Zuppi
authors.forename: Diana Valeria
authors.initials: DV
authors.lastname: Rossetti
authors.forename: Alberto
authors.initials: A
authors.lastname: Vitali
authors.forename: Federica
authors.initials: F
authors.lastname: Vincenzoni
authors.forename: Bruno
authors.initials: B
authors.lastname: Giardina
authors.forename: Massimo
authors.initials: M
authors.lastname: Castagnola
authors.forename: Irene
authors.initials: I
authors.lastname: Messana
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003404:Creatinine / Q000652:urine; D005919:Glomerular Filtration Rate; D006626:Hippurates / Q000652:urine*; D009682:Magnetic Resonance Spectroscopy; D015203:Reproducibility of Results
chemical_list: D006626:Hippurates; D003404:Creatinine; C030514:hippuric acid