Increasing oxygen tension improves filtration of sickle trait donor blood.
A major cause of filter failure of red cell (RBC) components from donors with sickle cell trait (HbAS) is the polymerization of haemoglobin. The oxygen saturation (sO2) of blood stored in various plastics and different volumes of air was assessed. Blood from 10 HbAS donors was collected and divided into two bags, one with air added, one without. Bags with added air had increased sO2 levels (from 49 +/- 10% to 76 +/- 6%). Filtration was successful for nine of 10 components with air, and one of 10 without air. Successful filtration of RBC components occurs when sO2 is increased.
Byrne
Karen M
KM
Department of Transfusion Medicine, National Institute of Diabetes and Digestive and Kidney Diseases/NIH, Warren G. Magnuson Clinical Center, Building 10, Room 1C711, 10 Center Drive MSC-1184, Bethesda, MD 20892-2284, USA. KByrne@mail.cc.nih.gov
Leitman
Susan F
SF
Schechter
Alan N
AN
Stroncek
David F
DF
D000293:Adolescent; D000328:Adult; D000388:Air; D001782:Blood Donors; D001793:Blood Preservation / Q000379:methods*; D002469:Cell Separation / Q000379:methods; D005374:Filtration; D006451:Hemoglobin, Sickle / Q000032:analysis; D006801:Humans; D007958:Leukocyte Count; D008875:Middle Aged; D010100:Oxygen / Q000097:blood*; D010313:Partial Pressure; D012805:Sickle Cell Trait / Q000097:blood*
D006451:Hemoglobin, Sickle; C028671:hemoglobin AS; D010100:Oxygen