alpha-cardiac actin (ACTC) binds to the band 3 (AE1) cardiac isoform.
The band 3 protein is the major integral protein present in the erythrocyte membrane. Two tissue-specific isoforms are also expressed in kidney alpha intercalated cells and in cardiomyocytes. It has been suggested that the cardiac isoform predominantly mediates the anion exchange in cardiomyocytes, but the role of the cytoplasmic domain of the band 3 (CDB3) protein in the cardiac tissue is unknown. In order to characterize novel associations of the CDB3 in the cardiac tissue, we performed the two-hybrid assay, using a bait comprising the region from leu 258 to leu 311 of the erythrocyte band 3, which must also be present in the cardiac isoform. The assay revealed two clones containing the C-terminal region of the alpha-cardiac actin. Immunoprecipitation of whole rat heart using an anti-actin antibody, immunoblotted with anti-human band 3, showed that actin binds to band 3 which was confirmed in the reverse assay. The confocal microscopy showed band 3 in the intercalated discs. Thus, besides the in vivo physical interaction in the Saccharomyces cerevisiae cell, we demonstrated using immunopreciptation that there is a physical association of band 3 with alpha-cardiac actin in cardiomyocyte, and we suggest that the binding occur "in situ," in the intercalated disc, a site of cell-cell contact and attachment of the sarcomere to the plasma membrane.
Moura Lima
Paulo Roberto
PR
Molecular and Cellular Biology Laboratory, Hemocentro, State University of Campinas, UNICAMP, Campinas, São Paulo, Brazil.
Salles
Teresa Sueko Ide
TS
Costa
Fernando Ferreira
FF
Saad
Sara Teresinha Olalla
ST
D000199:Actins / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001457:Anion Exchange Protein 1, Erythrocyte / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011233:Precipitin Tests; D020033:Protein Isoforms / Q000378:metabolism; D051381:Rats; D012441:Saccharomyces cerevisiae; D020798:Two-Hybrid System Techniques
D000199:Actins; D001457:Anion Exchange Protein 1, Erythrocyte; D020033:Protein Isoforms