title: Bioorganosolve pretreatments for simultaneous saccharification and fermentation of beech wood by ethanolysis and white rot fungi.
abstract: Ethanol was produced by simultaneous saccharification and fermentation (SSF) from beech wood chips after bioorganosolve pretreatments by ethanolysis and white rot fungi, Ceriporiopsis subvermispora, Dichomitus squalens, Pleurotus ostreatus, and Coriolus versicolor. Beech wood chips were pretreated with the white rot fungi for 2-8 weeks without addition of any nutrients. The wood chips were then subjected to ethanolysis to separate them into pulp and soluble fractions (SFs). From the pulp fraction (PF), ethanol was produced by SSF using Saccharomyces cerevisiae AM12 and a commercial cellulase preparation, Meicelase, from Trichoderma viride. Among the four strains, C. subvermispora gave the highest yield on SSF. The yield of ethanol obtained after pretreatment with C. subvermispora for 8 weeks was 0.294 g g(-1) of ethanolysis pulp (74% of theoretical) and 0.176 g g(-1) of beech wood chips (62% of theoretical). The yield was 1.6 times higher than that obtained without the fungal treatments. The biological pretreatments saved 15% of the electricity needed for the ethanolysis.
authors.affiliation: Laboratory of Biomass Conversion, Wood Research Institute, Kyoto University, Gokasho, Uji, Kyoto, Japan.
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Itoh
authors.forename: Masanori
authors.initials: M
authors.lastname: Wada
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Honda
authors.forename: Masaaki
authors.initials: M
authors.lastname: Kuwahara
authors.forename: Takashi
authors.initials: T
authors.lastname: Watanabe
mesh_terms: D001487:Basidiomycota / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology*; D002241:Carbohydrates / Q000096:biosynthesis*; D003246:Conservation of Energy Resources / Q000379:methods*; D000431:Ethanol / Q000378:metabolism*; D029964:Fagus / Q000737:chemistry*; D005285:Fermentation / Q000502:physiology; D007220:Industrial Waste / Q000517:prevention & control; D013696:Temperature; D014934:Wood
chemical_list: D002241:Carbohydrates; D007220:Industrial Waste; D000431:Ethanol