[Expression of hepatitis A virus procapsids in the insect cells infected by recombinant baculovirus].
The recombinant baculocvirus containing genome P1-2A-P3 of hepatitis A virus (HAV) was constructed and used for infecting the Sf9 insect cells. It was demonstrated that the deletion of 2BC from HAV polyprotein and the insertion of a new 3C protease cleavage site between P1-2A and P3 did not interfere with the processing of polyprotein or with forming the 70S-procapsids. The identity of the protein contents as well as of morphological and antigen characteristics, obtained in Sf9-cells, to HAV empty capsids, which take shape in the infected mammal cells, proves that it is possible to use them in making the vaccine and diagnostic preparations.
Dzagurov
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Kusov
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D000818:Animals; D016367:Baculoviridae / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D002213:Capsid / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D002460:Cell Line; D017353:Gene Deletion; D016679:Genome, Viral; D006506:Hepatitis A / Q000235:genetics / Q000378:metabolism*; D018936:Hepatitis C Antigens / Q000032:analysis / Q000378:metabolism; D015151:Immunoblotting; D007313:Insecta; D016253:Microscopy, Immunoelectron; D015202:Protein Engineering; D011498:Protein Precursors / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis; D011995:Recombination, Genetic
D018936:Hepatitis C Antigens; D011498:Protein Precursors; D011994:Recombinant Proteins