New integration vector using a cellulase gene as a screening marker for Lactobacillus.
The new integration vector for Lactobacillus, pJC4, was developed using the extracellular endoglucanase A gene (celA) of Clostridium thermocellum as a screening marker. pJC4 was transformed into four Lactobacillus species, Lb. johnsonii, Lb. gasseri, Lb. bulgaricus, and Lb. plantarum. In each species, the pJC4 integrants were easily and accurately detected by the appearance of a clear halo on a cellulase screening plate without any false transformants. Polymerase chain reaction and Southern hybridization indicated that all transformants with clear halos contained pJC4 in their chromosomal DNAs. The celA gene could be a useful screening marker for other lactic acid bacteria.
Jang
Soo Jin
SJ
School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Suwon 449-701, South Korea.
Ham
Min Seok
MS
Lee
Jung Min
JM
Chung
Sung Kyun
SK
Lee
Hyong Joo
HJ
Kim
Jeong Hwan
JH
Chang
Hae Choon
HC
Lee
Jong-Hoon
JH
Chung
Dae Kyun
DK
D002480:Cellulase / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods; D005820:Genetic Testing / Q000379:methods*; D007778:Lactobacillus / Q000235:genetics* / Q000302:isolation & purification; D008828:Microbiological Techniques; D014170:Transformation, Genetic
C074775:CelA endoglucanase; D002480:Cellulase