Functional regulation of biomolecule using DNA-conjugation.
3'-methacryloyl-oligodeoxynucleotide having antisense sequence against messenger RNA of green fluorescent protein and N-isopropylacrylamide were co-polymerized. The conjugate can reversibly change its conformation at around a transition temperature, similarly to N-isopropylacrylamide homopolymer. We achieved artificially regulation of gene expression depending on temperature using the conjugate.
Anada
Takahisa
T
Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 6-10-1, Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan.
Kano
Takeshi
T
Kaku
Wataru
W
Katayama
Yoshiki
Y
Maeda
Mizuo
M
Murata
Masaharu
M
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D016376:Oligonucleotides, Antisense / Q000737:chemistry
D016376:Oligonucleotides, Antisense; D004247:DNA