Enzymatic extraction of chondroitin sulfate from skate cartilage and concentration-desalting by ultrafiltration.
Skate cartilage is a fishery by-product, which contains chondroitin sulfate (CS), a glycosaminoglycan well known for its chondroprotective effect. Here described is a low-cost two-step process producing CS in non-denaturing conditions, consisting of an enzymatic extraction followed by tangential filtration to concentrate and purify CS. The performances of UF and MF membranes were compared in terms of flux and selectivity. The 0.1 microm-pore size membrane appeared to be the most efficient to separate CS from the other compounds.
Lignot
B
B
Laboratoire Polymères et Procédés (L2P), Centre de Recherches, Université de Bretagne Sud, rue Saint Maudé, F-56325 Lorient, France. brigitte.lignot@univ-ubs.fr
Lahogue
V
V
Bourseau
P
P
D000818:Animals; D002457:Cell Extracts / Q000737:chemistry / Q000302:isolation & purification; D002809:Chondroitin Sulfates / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D005240:Feasibility Studies; D006868:Hydrolysis; D017551:Microdialysis / Q000295:instrumentation / Q000379:methods*; D010206:Papain / Q000737:chemistry*; D012492:Salts / Q000737:chemistry*; D012860:Skates, Fish / Q000378:metabolism; D012996:Solutions; D014462:Ultrafiltration / Q000295:instrumentation / Q000379:methods*
D002457:Cell Extracts; D012492:Salts; D012996:Solutions; D002809:Chondroitin Sulfates; D010206:Papain