[HPLC method for determination of tramadol hydrochloride in human plasma].
OBJECTIVE
A reversed-phase high performance liquid chromatography (HPLC) method was established to determine the concentration of tramadol hydrochloride in human plasma.


METHODS
HPLC instrument was used with the column: 150 mm x 4.6 mm (Li Chrosorb C18, 5 microns). The mobile phase was composed of phosphate buffer solution (0.02 mol/L, pH = 3.7)-acetamide (83: 17, V/V). Flow rate was 1.0 ml/min. Detection wavelength was 216 nm.


RESULTS
The standard curve equation was Y = 0.03244X + 0.6007. The linear range was 25-800 ng/ml. The minimum detection limit was 10.2 ng/ml.


CONCLUSIONS
The reversed-phase HPLC method is simple, rapid and sensitive. It is applicable to the determination of the concentration of tramadol hydrochloride in human plasma.
Qu
Lingbo
L
Department of Chemistry, Zhengzhou University, Zhengzhou 450052, China.
Feng
Shuhui
S
Wu
Yongjun
Y
Wu
Yuming
Y
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D006801:Humans; D014147:Tramadol / Q000097:blood*
D014147:Tramadol