title: [Identification of protein spots in silver-stained two-dimensional gels based on chemically assisted fragmentation by MALDI-TOF-PSD mass spectrometry].
abstract: Guanidination of the epsilon-amino group of lysine-terminated tryptic peptides,accomplished with O-methylisourea hydrogen sulfate, converted lysine into homoarginine residues, improving detection in MALDI-MS. Then tryptic peptides were labeled with sulfonic acid groups at the N-termini using 3-sulfopropionic acid NHS-ester chemistry. The derivatives enhanced post-source decay (PSD) fragmentation signals and produced a spectrum containing only y-ions. This facilitated de novo peptide sequencing, so it is an important contribution to unambiguous protein identification in proteome research. This method was successfully applied in nasopharyngeal carcinoma(NPC) proteome study.
authors.affiliation: College of Life Science, Hunan Normal University, Changsha 410081, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.forename: Song
authors.initials: S
authors.lastname: Nie
authors.forename: Jin-Yun
authors.initials: JY
authors.lastname: Xie
authors.forename: Song-Ping
authors.initials: SP
authors.lastname: Liang
authors.forename: Chong
authors.initials: C
authors.lastname: Peng
authors.forename: Gui-Yuan
authors.initials: GY
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D015180:Electrophoresis, Gel, Two-Dimensional; D008969:Molecular Sequence Data; D011506:Proteins / Q000032:analysis*; D016622:Silver Staining; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D011506:Proteins