Thermodynamic analysis of hybridization between POPNA and DNA.
We have developed a new series of oxy-peptide nucleic acids with pyrrolidine rings of four different types of structural isomers (cis L, cis D, trans L, trans D configurations; POPNAs). Melting curves of adenine nanomers of POPNAs in the presence of the complementary DNA showed the highest Tm value for cis L-POPNA, but in the case of RNA, the highest Tm value was observed for trans L-POPNA. Thermodynamic parameters (delta H and delta S) of POPNA/DNA and POPNA/RNA hybridizations were evaluated from concentration dependence of Tm values. The POPNA/DNA and POPNA/RNA hybridizations were governed by entropy and enthalpy terms, respectively.
Kitamatsu
Mizuki
M
Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, Okayama 700-8530, Japan.
Shigeyasu
Masanori
M
Okada
Tomoyuki
T
Nakai
Takashi
T
Saitou
Mamoru
M
Sisido
Masahiko
M
D004247:DNA / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D011759:Pyrrolidines / Q000737:chemistry; D013816:Thermodynamics
D020135:Peptide Nucleic Acids; D011759:Pyrrolidines; D004247:DNA; C032519:pyrrolidine