Resemblance and dissemblance of Arabidopsis type II peroxiredoxins: similar sequences for divergent gene expression, protein localization, and activity.
The Arabidopsis type II peroxiredoxin (PRXII) family is composed of six different genes, five of which are expressed. On the basis of the nucleotide and protein sequences, we were able to define three subgroups among the PRXII family. The first subgroup is composed of AtPRXII-B, -C, and -D, which are highly similar and localized in the cytosol. AtPRXII-B is ubiquitously expressed. More striking is the specific expression of AtPRXII-C and AtPRXII-D localized in pollen. The second subgroup comprises the mitochondrial AtPRXII-F, the corresponding gene of which is expressed constitutively. We show that AtPRXII-E, belonging to the last subgroup, is expressed mostly in reproductive tissues and that its product is addressed to the plastid. By in vitro enzymatic experiments, we demonstrate that glutaredoxin is the electron donor of recombinant AtPRXII-B for peroxidase reaction, but the donors of AtPRXII-E and AtPRXII-F have still to be identified.
Bréhélin
Claire
C
Laboratoire Génome et Développement des Plantes, Université de Perpignan, Unité Mixte de Recherche Centre National de la Recherche Scientifique 5096, 52 avenue de Villeneuve, 66860 Perpignan, France.
Meyer
Etienne H
EH
de Souris
Jean-Paul
JP
Bonnard
Géraldine
G
Meyer
Yves
Y
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002736:Chloroplasts / Q000201:enzymology; D017124:Conserved Sequence; D003600:Cytosol / Q000201:enzymology; D015870:Gene Expression; D020869:Gene Expression Profiling; D017343:Genes, Plant / Q000235:genetics; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010544:Peroxidases / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment
D029681:Arabidopsis Proteins; D012333:RNA, Messenger; D010544:Peroxidases