title: A validated 1H NMR method for the determination of the degree of deacetylation of chitosan.
abstract: A method for the determination of the degree of deacetylation (DDA) of chitosan by 1H NMR spectroscopy has been formally validated. Chitosans with DDA ranging from 48 to 100% have been used for the validation. The method is found to be simple, rapid and more precise than other known techniques like IR or titration for %DDA measurements. The precision, ruggedness, robustness, specificity, stability and accuracy of the technique are discussed in this paper.
authors.affiliation: Department of Chemical Engineering, Institute of Biomedical and Chemical Engineering, Ecole Polytechnique of Montréal, PO Box 6079, Station Center-Ville, Québec, Montréal, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Lavertu
authors.forename: Z
authors.initials: Z
authors.lastname: Xia
authors.forename: A N
authors.initials: AN
authors.lastname: Serreqi
authors.forename: M
authors.initials: M
authors.lastname: Berrada
authors.forename: A
authors.initials: A
authors.lastname: Rodrigues
authors.forename: D
authors.initials: D
authors.lastname: Wang
authors.forename: M D
authors.initials: MD
authors.lastname: Buschmann
authors.forename: Ajay
authors.initials: A
authors.lastname: Gupta
mesh_terms: D000107:Acetylation; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry*; D048271:Chitosan; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D011522:Protons; D015203:Reproducibility of Results
chemical_list: D011522:Protons; D002686:Chitin; D048271:Chitosan