Solid phase synthesis of phosphorothioate oligonucleotides utilizing diethyldithiocarbonate disulfide (DDD) as an efficient sulfur transfer reagent.
Diethyldithiodicarbonate (DDD), a cheap and easily prepared compound, is found to be a rapid and efficient sulfurizing reagent in solid phase synthesis of phosphorothioate oligodeoxyribonucleotides via the phosphoramidite approach. Product yield and quality based on IP-LC-MS compares well with high quality oligonucleotides synthesized using phenylacetyl disulfide (PADS) which is being used for manufacture of our antisense drugs.
Cheruvallath
Zacharia S
ZS
Isis Pharmaceuticals, Carlsbad, California 92008, USA.
Kumar
R Krishna
RK
Rentel
Claus
C
Cole
Douglas L
DL
Ravikumar
Vasulinga T
VT
D002254:Carbonates / Q000737:chemistry*; D007202:Indicators and Reagents; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D013440:Sulfides / Q000737:chemistry*; D013455:Sulfur / Q000737:chemistry*; D013873:Thionucleotides / Q000138:chemical synthesis* / Q000737:chemistry
D002254:Carbonates; D007202:Indicators and Reagents; D009841:Oligonucleotides; D013440:Sulfides; D013873:Thionucleotides; C482610:diethyldithiodicarbonate disulfide; D013455:Sulfur