Hypothermic preservation of hepatocytes.
This paper presents a review of recent research on the hypothermic storage of hepatocytes. The first focus is on the diversity of methodologies currently employed in this area. The cell damage caused by hypothermic preservation and its possible mechanism are then investigated on both morphological and molecular biology. Later, the gene expressions on a mRNA level or enzyme level after hypothermic preservation are further discussed. Finally, the improvement of hypothermic storage by preconditioning, such as by increasing temperature, is explored.
Meng
Qin
Q
Department of Chemical Engineering and Biochemical Engineering, Zhejiang University, 38 Zheda Road, Hangzhou, Zhejiang, P. R. China. zhang_meng@hzcnc.com
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods* / Q000639:trends; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D015925:Cryopreservation / Q000379:methods* / Q000639:trends*; D005786:Gene Expression Regulation / Q000502:physiology*; D006360:Heat-Shock Proteins / Q000378:metabolism*; D022781:Hepatocytes / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D007036:Hypothermia, Induced / Q000379:methods / Q000639:trends; D051379:Mice; D014021:Tissue Preservation / Q000379:methods / Q000639:trends
D000975:Antioxidants; D006360:Heat-Shock Proteins