Tissue- and stressor-specific differential expression of two hsc70 genes in carp.
Two genes expressing 70 kDa heat shock proteins were identified in Cyprinus carpio. The sequence similarities and the intron-interrupted structure of the coding regions indicate that carp Hsc70-1 and Hsc70-2 belong to the Hsp70 cognate subfamily. The expressions of the two hsc70 genes were followed by semi-quantitative RT-PCR. Both genes are expressed under unstressed conditions in a characteristic tissue-specific manner. Inducibility of the response to elevated temperature, cold shock, and Cd treatment was investigated in the liver and muscle, in whole-animal experiments. Both genes were insensitive to or only weakly induced by the stressors, with two exceptions: Cd treatment resulted in an 11-13-fold enhanced induction of hsc70-1 in the liver and cold shock enhanced induction of hsc70-2 in the muscle by 7.5-10-fold.
Ali
Khaled Said
KS
Department of Biochemistry, Faculty of Science, University of Szeged, PO Box 533, H-6701, Szeged, Hungary.
Dorgai
László
L
Abrahám
Magdolna
M
Hermesz
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D000595:Amino Acid Sequence; D000818:Animals; D002104:Cadmium / Q000494:pharmacology; D002347:Carps / Q000378:metabolism*; D005786:Gene Expression Regulation; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000145:classification / Q000235:genetics; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D009132:Muscles / Q000378:metabolism; D009928:Organ Specificity; D010802:Phylogeny; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics; D016415:Sequence Alignment; D013696:Temperature; D014018:Tissue Distribution
D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D020033:Protein Isoforms; D002104:Cadmium