[Determination of protein concentration by the enhancement of Rayleigh light scattering of fuchsine acid].
A new Rayleigh light scattering (RLS) assay is presented in this paper. At the optimum pH = 2.72, the weak RLS of fuchsine acid can be greatly enhanced by the addition of proteins due to the interaction between protein and fuchsine acid. A new quantitative determination method for proteins has been developed. The linear range for human serum albumin is 0-4.0 mg.L-1 with detection limit of 23 micrograms.L-1. Besides high sensitivity, the method is characterized by good reproducibility, rapidity of reaction, good stability and few interfering substances. The determination results of the proteins in human serum and urine samples are very close those obtained using Biuret method, with relative stand deviation of 0.94%-4.93%.
Zhang
Hong-yi
HY
College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China.
Liu
Bao-sheng
BS
Zhang
Hong-lei
HL
Zhao
Yong
Y
D001557:Benzenesulfonates; D011506:Proteins / Q000032:analysis; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D012709:Serum Albumin / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D001557:Benzenesulfonates; D011506:Proteins; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; C086337:acid fuchsin