Influence of alginate characteristics on the properties of multi-component microcapsules.
A variety of sodium alginates, differing in molar mass and structural composition, have been evaluated in the preparation of multi-component microbeads and microcapsules. Bead formation occurred by gelation with calcium chloride. Capsules were produced by reacting the pre-formed beads with the oligocation poly(methylene-co-guanidine). Despite the equiponderous (1:1) mixing with a second polyanion, sodium cellulose sulphate, the influence of the alginate properties remains evident. Specifically, the effect of the chemical composition was found to be more significant than that of the molar mass for both the mechanical and transport properties. Furthermore, for alginates of 73% alpha-l-guluronic acid content less shrinking was observed compared to the 38% guluronic materials. This results in the case of the same encapsulator settings in larger microsphere diameters and thicker membranes accompanied by enhanced mechanical resistance though, also, in a higher permeability for the high-G capsules. However, subsequent coating with lower molar mass alginate allows one to adjust the permeability over a broad range, suitable for cell encapsulation and immunoprotection, without compromising the durability.
Wandrey
C
C
Institute of Chemical and Biological Process Science, Swiss Federal Institute of Technology, Lausanne, Switzerland. christine.wandrey@epfl.ch
Espinosa
D
D
Rehor
A
A
Hunkeler
D
D
D000464:Alginates / Q000737:chemistry*; D001672:Biocompatible Materials; D002118:Calcium; D002214:Capsules; D003911:Dextrans; D004339:Drug Compounding / Q000379:methods; D008863:Microspheres; D010316:Particle Size; D010539:Permeability; D010784:Photomicrography / Q000379:methods; D011108:Polymers; D012964:Sodium; D012996:Solutions; D014783:Viscosity
D000464:Alginates; D001672:Biocompatible Materials; D002214:Capsules; D003911:Dextrans; D011108:Polymers; D012996:Solutions; D012964:Sodium; D002118:Calcium