[Expression and purification of gene transfer vehicle mediated by epidermal growth factor receptor].
OBJECTIVE
To create a gene transfer vehicle for targeting gene therapy of cancer with epidermal growth factor receptor overexpressing.


METHODS
Encoding sequences of the first domain of histone gene (H1) and EGF C-loop (EGFc) were obtained by PCR amplification. These two DNA fragments were ligated by EcoR I site, and cloned and sequenced. E. coli expression vector of the fusion gene was then constructed. The fusion protein H1EGFc was purified by specific band isolation from SDS-PAGE.


RESULTS
The molecular weight of purified protein was consistent with the designed request. Its purity reached 94.02%.


CONCLUSIONS
A fusion protein H1EGFc was expressed and purified.
Fang
Hua-sheng
HS
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China.
Zhang
Lin
L
Lu
Sheng-dong
SD
Zhang
Shu-zheng
SZ
D000595:Amino Acid Sequence; D001483:Base Sequence; D066246:ErbB Receptors / Q000096:biosynthesis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors; D006657:Histones / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D015202:Protein Engineering; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification*
D006657:Histones; D011993:Recombinant Fusion Proteins; D066246:ErbB Receptors