Evaluation of susceptibility of human herpesvirus 8 to antiviral drugs by quantitative real-time PCR.
A new in vitro system based on real-time PCR was developed for evaluation of human herpesvirus 8 susceptibility to antiviral agents. Cidofovir had the greatest inhibitory activity against HHV-8 (50% inhibitory concentration [IC(50)], 0.43 microM) followed by ganciclovir (2.61 microM), adefovir (18.00 microM), acyclovir (31.00 microM), and foscarnet (34.15 microM). The potential therapeutic efficacy for HHV-8 (i.e., peak serum drug level/IC(50)) is highest for cidofovir (167) and foscarnet (22).
Sergerie
Yan
Y
Centre de Recherche en Infectiologie, Centre Hospitalier Universitaire de Québec (Pavillon CHUL), and Université Laval, Québec, Canada.
Boivin
Guy
G
D000998:Antiviral Agents / Q000097:blood / Q000494:pharmacology*; D000077404:Cidofovir; D003596:Cytosine / Q000031:analogs & derivatives* / Q000097:blood / Q000494:pharmacology*; D015774:Ganciclovir / Q000097:blood / Q000494:pharmacology; D019288:Herpesvirus 8, Human / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D008826:Microbial Sensitivity Tests; D063065:Organophosphonates; D009943:Organophosphorus Compounds / Q000097:blood / Q000494:pharmacology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012044:Regression Analysis
D000998:Antiviral Agents; D063065:Organophosphonates; D009943:Organophosphorus Compounds; D003596:Cytosine; D000077404:Cidofovir; D015774:Ganciclovir