title: Effects of intravenous apolipoprotein A-I/phosphatidylcholine discs on LCAT, PLTP, and CETP in plasma and peripheral lymph in humans.
abstract: OBJECTIVE
  We have previously shown that intravenous apolipoprotein A-I/phosphatidylcholine (apoA-I/PC) discs increase plasma pre-beta HDL concentration and stimulate reverse cholesterol transport (RCT) in humans. We have now investigated the associated changes in the following 3 HDL components that play key roles in RCT: lecithin:cholesterol acyltransferase (LCAT), cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP).
  
  
  RESULTS
  apoA-I/PC discs (40 mg/kg over 4 hours) were infused into 8 healthy men. Samples of blood and prenodal peripheral lymph were collected for 24 to 48 hours. At 12 hours, plasma LCAT concentration had increased by 0.40+/-0.90 mg/L (+7.8%; mean+/-SD; P<0.05), plasma cholesterol esterification rate by 29.0+/-9.0 nmol/mL per h (+69.5%; P<0.01), plasma CETP concentration by 0.5+/-0.2 mg/L (+29.7%; P<0.01), and plasma PLTP activity by 1.45+/-0.67 micromol/mL per h (+23.9%; P<0.01). In contrast, plasma PLTP concentration had decreased by 4.4+/-2.7 mg/L (-44.8%; P<0.01). The changes in PLTP were accompanied by alterations in the relative proportions of large lipoproteins containing inactive PLTP and small particles containing PLTP of high specific activity. No changes were detected in peripheral lymph.
  
  
  CONCLUSIONS
  Nascent HDL secretion may induce changes in PLTP, LCAT, and CETP that promote RCT by catalyzing pre-beta HDL production, cholesterol esterification in HDLs, and cholesteryl ester transfer from HDLs to other lipoproteins.
authors.affiliation: Department of Advanced Medical Technology and Development, BML, Inc, 1361-1 Matoba, Kawagoe, Saitama 350-1101, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kujiraoka
authors.forename: M Nazeem
authors.initials: MN
authors.lastname: Nanjee
authors.forename: Tomoichiro
authors.initials: T
authors.lastname: Oka
authors.forename: Mayumi
authors.initials: M
authors.lastname: Ito
authors.forename: Makoto
authors.initials: M
authors.lastname: Nagano
authors.forename: C Justin
authors.initials: CJ
authors.lastname: Cooke
authors.forename: Sadao
authors.initials: S
authors.lastname: Takahashi
authors.forename: Waldemar L
authors.initials: WL
authors.lastname: Olszewski
authors.forename: Jinny S
authors.initials: JS
authors.lastname: Wong
authors.forename: Irina P
authors.initials: IP
authors.lastname: Stepanova
authors.forename: Robert L
authors.initials: RL
authors.lastname: Hamilton
authors.forename: Tohru
authors.initials: T
authors.lastname: Egashira
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Hattori
authors.forename: Norman E
authors.initials: NE
authors.lastname: Miller
mesh_terms: D000328:Adult; D016632:Apolipoprotein A-I / Q000008:administration & dosage* / Q000494:pharmacology*; D001053:Apolipoproteins / Q000097:blood; D001798:Blood Proteins / Q000378:metabolism*; D002352:Carrier Proteins / Q000097:blood / Q000378:metabolism; D053480:Cholesterol Ester Transfer Proteins; D003692:Delayed-Action Preparations / Q000008:administration & dosage / Q000494:pharmacology; D016503:Drug Delivery Systems; D006023:Glycoproteins; D006801:Humans; D007262:Infusions, Intravenous; D008055:Lipids / Q000097:blood; D008074:Lipoproteins / Q000648:ultrastructure; D008196:Lymph / Q000737:chemistry*; D008297:Male; D008565:Membrane Proteins / Q000097:blood / Q000378:metabolism; D008854:Microscopy, Electron; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000097:blood / Q000378:metabolism; D010713:Phosphatidylcholines / Q000008:administration & dosage* / Q000494:pharmacology*; D049294:Phospholipid Transfer Proteins
chemical_list: D016632:Apolipoprotein A-I; D001053:Apolipoproteins; D001798:Blood Proteins; C506642:CETP protein, human; D002352:Carrier Proteins; D053480:Cholesterol Ester Transfer Proteins; D003692:Delayed-Action Preparations; D006023:Glycoproteins; D008055:Lipids; D008074:Lipoproteins; D008565:Membrane Proteins; D010713:Phosphatidylcholines; D049294:Phospholipid Transfer Proteins; D007862:Phosphatidylcholine-Sterol O-Acyltransferase