title: [Study on the effect of integrin on human sperm activation].
abstract: OBJECTIVE
  This study was aimed to disclose whether the VLA-5 integrin on human sperm membrane takes part in the activation of human spermatozoa.
  
  
  METHODS
  Fluo-3/AM was coincubated with human spermatozoa. The change of intracellular calcium([Ca2+]i) was determined by flow cytometry when human sperms were exposed to Fibronectin(Fn) as the specific ligand of integrin VLA-5. Human sperm acrosome reaction(AR) rate was evaluated by triple-stain technique.
  
  
  RESULTS
  Fn significantly raised the AR rate (P < 0.05) and the level of [Ca2+]i (P < 0.01) in the spermatozoa of fertile men. But there was no significant rise in the level of [Ca2+]i in the spermatozoa of infertile men.
  
  
  CONCLUSIONS
  The VLA-5 integrin on human sperm membrane plays a role in the activation of sperm.
authors.affiliation: Department of Physiology, West China School of Basic and Forensic Medical Sciences, Sichuan University, Chengdu 610041, China.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Xie
authors.forename: Limin
authors.initials: L
authors.lastname: Yue
authors.forename: Yaping
authors.initials: Y
authors.lastname: He
authors.forename: Jinhu
authors.initials: J
authors.lastname: Zhang
authors.forename: Rongqiu
authors.initials: R
authors.lastname: Mao
authors.forename: Song
authors.initials: S
authors.lastname: Lei
authors.forename: Yu
authors.initials: Y
authors.lastname: Zheng
mesh_terms: D020101:Acrosome Reaction / Q000187:drug effects*; D000328:Adult; D002118:Calcium / Q000378:metabolism; D006801:Humans; D039081:Integrin alpha5beta1 / Q000502:physiology*; D008297:Male; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000378:metabolism
chemical_list: D039081:Integrin alpha5beta1; D002118:Calcium