Immunosuppressive effects of soluble factors secreted by head and neck squamous cell carcinoma on dendritic cells and T lymphocytes.
Recent observations suggest that the inability of the immune system to mount an effective immune response against head and neck squamous cell carcinoma (HNSCC) could be a result of the immunosuppression mediated through soluble factors that are secreted by tumour cells. Therefore, we investigated the effects of conditioned medium obtained from cultures of HNSCC cell lines (HNSCC-CM) on the function of dendritic cells (DC) and T cell immune response. In our study, we could not observe any inhibitory effect of HNSCC-CM on the maturation and the cytokine secretion pattern of DC. On the contrary, HNSCC-CM from two of three cell lines consistently decreased the quantity of IFN-gamma- and IL-4-secreting T cells upon restimulation in vitro. In conclusion, our data suggest that soluble factors secreted by HNSCC cells directly inhibit the function of effector T cells, rather than impeding the process of antigen presentation.
Kacani
Laco
L
Department of Otorhinolaryngology, University Hospital Innsbruck, Anichstrasse 35, A-6020 Innsbruck, Austria.
Wurm
Martin
M
Schennach
Harald
H
Braun
Ilona
I
Andrle
Jan
J
Sprinzl
Georg M
GM
D017951:Antigen Presentation; D001685:Biological Factors / Q000276:immunology; D002294:Carcinoma, Squamous Cell / Q000276:immunology*; D002454:Cell Differentiation / Q000276:immunology; D017077:Culture Media, Conditioned; D016207:Cytokines / Q000096:biosynthesis; D003713:Dendritic Cells / Q000276:immunology*; D006258:Head and Neck Neoplasms / Q000276:immunology*; D006801:Humans; D007108:Immune Tolerance; D016753:Interleukin-10 / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism; D012995:Solubility; D013601:T-Lymphocytes / Q000276:immunology*; D014407:Tumor Cells, Cultured; D019139:Tumor Escape / Q000276:immunology*
D001685:Biological Factors; D017077:Culture Media, Conditioned; D016207:Cytokines; D016753:Interleukin-10; D018664:Interleukin-12