Modification of the double-disk test for detection of enterobacteriaceae producing extended-spectrum and AmpC beta-lactamases.
Detection of extended-spectrum beta-lactamases (ESBLs) in AmpC-producing Enterobacteriaceae is problematic. A modification of the double-disk test (MDDT) has been developed for successful detection of ESBLs in gram-negative bacilli producing well-characterized beta-lactamases as well as 212 clinical isolates of Enterobacter cloacae, Enterobacter aerogenes, Serratia marcescens, and Citrobacter freundii. MDDT accurately differentiated between ESBL producers and derepressed chromosomal AmpC mutants. MDDT provides a cost-effective alternative approach for clinical microbiology laboratories for routine susceptibility testing with simultaneous detection of ESBLs in enterobacteriaceae.
Pitout
J D D
JD
Department of Pathology & Laboratory Medicine, University of Calgary, Calgary T3C 0J5, Canada. johann.pitout@cls.ab.ca
Reisbig
M D
MD
Venter
E C
EC
Church
D L
DL
Hanson
N D
ND
D001426:Bacterial Proteins; D004755:Enterobacteriaceae / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D004926:Escherichia coli / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification; D006801:Humans; D007753:Laboratories; D010641:Phenotype; D015203:Reproducibility of Results; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; C117199:beta-lactamase TEM-61; C048795:AmpC beta-lactamases; D001618:beta-Lactamases