title: Intramolecular dimers: a new design strategy for fluorescence-quenched probes.
abstract: Fluorogenic probes dual-labeled with reporter and quencher dyes use a change in fluorescence to monitor biochemical events (e.g., substrate binding or enzyme digestion). Such events change the reporter-quencher distance, which affects fluorescence. Recently, it is has been shown that static quenching through intramolecular dimers is an important mechanism that can sometimes be more efficient than Förster resonance energy transfer (FRET).
authors.affiliation: Biosearch Technologies, 81 Digital Drive, Novato, CA 94949, USA. info@biosearchtech.com
authors.forename: Mary Katherine
authors.initials: MK
authors.lastname: Johansson
authors.forename: Ronald M
authors.initials: RM
authors.lastname: Cook
mesh_terms: D002232:Carbocyanines / Q000737:chemistry; D019281:Dimerization; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D015345:Oligonucleotide Probes / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D012235:Rhodamines / Q000737:chemistry
chemical_list: D002232:Carbocyanines; D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D010455:Peptides; D012235:Rhodamines; C085321:cyanine dye 5