A simple differential pulse polarographic method for the determination of thymoquinone in black seed oil.
A reliable and simple differential pulse polarographic method is described for the determination of thymoquinone in black seed oil. The polarographic behaviour of thymoquinone was examined in various buffer systems over the pH range 5.0-10.0. Thymoquinone is reduced in a single, reversible peak at the dropping mercury electrode. The differential pulse polarogram showed a distinct peak in Sörensen buffer:methanol (3:7, v/v; pH 8.5) at a peak potential of -0.095 V (vs. silver/silver chloride electrode), and a plot of peak height against concentration was found to be linear over the range 0.2-15.0 microg/mL (R = 0.9998). The limit of detection was calculated to be 0.054 microg/mL. The polarographic method has been applied to determine thymoquinone in two black seed oil preparations available on the Austrian pharmaceutical market.
Michelitsch
Astrid
A
Institut für Pharmazeutische Chemie und Pharmazeutische Technologie der Karl-Franzens-Universität Graz, Schubertstrasse 1, A-8010 Graz, Austria. astrid.ortner@kfunigraz.ac.at
Rittmannsberger
Anna
A
D016227:Benzoquinones / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D008954:Models, Biological; D031881:Nigella sativa / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D010938:Plant Oils / Q000737:chemistry* / Q000302:isolation & purification; D011048:Polarography / Q000379:methods*; D012639:Seeds / Q000737:chemistry*
D016227:Benzoquinones; D010936:Plant Extracts; D010938:Plant Oils; C003466:thymoquinone