title: [Correlation analysis between WNK4 gene and essential hypertension].
abstract: OBJECTIVE
  To investigate association of mutation in WNK4 gene with essential hypertension and to analyze the expression of WNK4 gene.
  
  
  METHODS
  cSNP in WNK4 gene in a small samples was detected by sequencing, then PCR-RFLP was performed in 98 patients with essential hypertension and 95 control subjects. The expression profile of WNK4 gene was tested by RT-PCR.
  
  
  RESULTS
  A cSNP was detected in WNK4 gene exon7 G1662A, and there were significant differences in the distribution of allele frequency of G1662A between essential hypertension group and control group. WNK4 gene were expressed in the tissues of kidney, brain, lung, heart, spleen and intestine of fetus.
  
  
  CONCLUSIONS
  WNK4 gene is well correlated with essential hypertension.
authors.affiliation: Department of Genetics, China Medical University, Shenyang 110001, China.
authors.forename: Zhi-jun
authors.initials: ZJ
authors.lastname: Sun
authors.forename: Xin-nan
authors.initials: XN
authors.lastname: Wang
authors.forename: Jing-yu
authors.initials: JY
authors.lastname: Lu
authors.forename: Qian
authors.initials: Q
authors.lastname: Ding
authors.forename: Ling-yue
authors.initials: LY
authors.lastname: Dong
authors.forename: Yan-yan
authors.initials: YY
authors.lastname: Zhao
mesh_terms: D000328:Adult; D001921:Brain / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D005787:Gene Frequency; D005796:Genes; D020022:Genetic Predisposition to Disease; D006801:Humans; D006973:Hypertension / Q000235:genetics*; D007668:Kidney / Q000378:metabolism; D008239:Lysine / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009154:Mutation; D010641:Phenotype; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D017346:Protein Serine-Threonine Kinases; C433806:WNK4 protein, human; D008239:Lysine