Characterization of RNA structure by bis-pyrene-labeled 2'-O-methyloligonucleotides.
Properties of 2'-O-methyloligoribonucleotides containing two consecutive 2'-O-(1-pyrenylmethyl)uridine were investigated as a fluorescent probe to search the single strand regions of RNA. The bis-pyrene-labeled 2'-O-methyloligoribonucleotide (OMUpy2) induced the formation of pyrene dimer upon hybridization with the complementary oligoribonucleotides and showed remarkable appearance of broad structureless fluorescence at 480 nm. Contrarily, when OMUpy2 was hybridized with the complementary oligodeoxyribonucleotides, such enhancement of fluorescence was scarcely observed. When various OMUpy2 were applied to E. coli 5S-rRNA, the fluorescence intensity at 480 nm was varied in a sequence specific manner.
Mahara
A
A
Department of Polymer Science and Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
Iwase
R
R
Yamana
K
K
Yamaoka
T
T
Murakami
A
A
D001483:Base Sequence; D005456:Fluorescent Dyes; D015394:Molecular Structure; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000138:chemical synthesis / Q000737:chemistry*; D020320:Oligoribonucleotides, Antisense / Q000138:chemical synthesis / Q000737:chemistry; D011721:Pyrenes / Q000737:chemistry; D012313:RNA / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D020320:Oligoribonucleotides, Antisense; D011721:Pyrenes; D012313:RNA