Kinetics of beta-glucosidase production by Saccharomyces cerevisiae recombinants harboring heterologous bgl genes.
The maximum productivity of beta-glucosidase by Saccharomyces cerevisiae recombinants under the control of GALI promoter was 100 IU l(-1) h(-1). The highest productivity of beta-glucosidase by a S. cerevisiae recombinant was 16-fold more than that supported by Cellulomonas biazotea. The recombinants also co-produced ethanol from cellobiose: maximum product yield and productivity were 0.5 and 1.1 g ethanol g(-1) cellobiose and g ethanol l(-1) h(-1), respectively.
Rajoka
M I
MI
National Institute for Biotechnology and Genetic Engineering, P.O. Box 577, Jhang Road, Faisalabad, Pakistan. mirajoka@nibge.org
Shaukat
Farhana
F
Ghauri
M T
MT
Shahid
Riaz
R
D002475:Cellobiose / Q000378:metabolism; D040143:Cellulomonas / Q000201:enzymology / Q000235:genetics; D003001:Cloning, Molecular; D004789:Enzyme Activation; D000431:Ethanol / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D015966:Gene Expression Regulation, Fungal / Q000235:genetics; D007700:Kinetics; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D013045:Species Specificity; D001617:beta-Glucosidase / Q000096:biosynthesis* / Q000235:genetics*
D011994:Recombinant Proteins; D002475:Cellobiose; D000431:Ethanol; D001617:beta-Glucosidase