[Changes of handling of calcium in mesenteric vascular smooth muscle cells of calcium overload rat].
OBJECTIVE
To investigate if there existed abnormal handling of calcium in vascular smooth muscle cells (VSMC) in calcium overload rat (CaOMV).


METHODS
Cytosolic and nuclear calcium ion handling in mesenteric VSMC both in calcium overload rat and in Wistar rat were studied by using laser scanning confocal microscopy together with calcium-sensitive-dye Fluo-3/AM.


RESULTS
The results showed that both nuclear and cytosolic calcium level ([Ca2+]n, [Ca2+]i) in mesenteric VSMC in calcium overload rat under resting condition was not higher than that in normal rat. However, [Ca2+]n and [Ca2+]i in CaOMV increased more significantly than that in normal rat when induced by drugs such as KCl, Bay-k8644, Ang-II, IP3, CHCl3, and CPA.


CONCLUSIONS
(1) There exists abnormal handling of calcium in CaOMV because of it's overreaction to stimulators, which indicate that intracellular and extracellular calcium overload coexist in age-related arteriosclerosis; (2) Not only Ca2+ gating system of plasma membrane but also calcium pool in CaOMV are irregular; (3) Cytosolic calcium as well as nuclear calcium are handled improperly in CaOMV.
Zhang
X
X
Department of Physiology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China.
Xu
X
X
Wen
Y
Y
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D008297:Male; D008643:Mesentery / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D002118:Calcium