The Streptomyces coelicolor ssgB gene is required for early stages of sporulation.
ssgB was identified as a novel early sporulation gene in Streptomyces coelicolor. An ssgB deletion mutant failed to sporulate, over-produced actinorhodin, and its colonies were significantly larger than those of the parental strain, suggesting an important role for the ssgB gene product in the process of growth cessation prior to sporulation-specific cell division. This places ssgB temporally before the paralogous sporulation gene ssgA. Analysis of ssgB mutant hyphae by electron microscopy and by confocal fluorescence microscopy showed that it was defective in the initiation of sporulation, as no sporulation septa could be identified, and DNA segregation had not yet been initiated in the mutant.
Keijser
Bart J F
BJ
Department of Biochemistry, Leiden Institute of Chemistry, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands.
Noens
Elke E E
EE
Kraal
Barend
B
Koerten
Henk K
HK
van Wezel
Gilles P
GP
D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology*; D017353:Gene Deletion; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D010641:Phenotype; D012965:Sodium Chloride; D013171:Spores, Bacterial / Q000235:genetics / Q000502:physiology / Q000648:ultrastructure; D013302:Streptomyces / Q000235:genetics* / Q000502:physiology* / Q000648:ultrastructure
D001426:Bacterial Proteins; C462919:ssgB protein, Streptomyces coelicolor; D012965:Sodium Chloride