Lytic infection of pseudorabies virus in the presence of spermine, spermidine, or DFMO.
The effects of polyamines (spermine or spermidine) and DFMO (an ornithine decarboxylase inhibitor) on the infection of LM (tk-) cells by pseudorabies virus (PRV) were investigated. Results from radioactive methionine labeling showed that the synthesis of viral proteins was not affected; however, the expression of a distinctive cellular protein ( approximately 27 kDa) was induced after the treatment of spermine or spermidine. Using plaque assay, we found that the plaque formation of PRV was not affected by these three reagents either. Furthermore, the effects of these drugs on the transcription of PRV immediate-early gene (IE) promoter were examined by CAT assay, and results showed weak stimulation of transcription by these drugs. Taken together, our results demonstrated that lytic infection of PRV was not influenced by addition of exogenous polyamines or depletion of endogenous polyamines; this conclusion was similar to earlier studies by using herpes simplex virus.
Wang
Hsien-Chi
HC
Department of Veterinary Medicine, College of Veterinary Medicine, National Chung-Hsing University, Taichung, 402, Taiwan.
Wong
Min-Liang
ML
D000818:Animals; D015317:Biogenic Polyamines / Q000378:metabolism* / Q000494:pharmacology*; D002460:Cell Line; D000518:Eflornithine / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000494:pharmacology; D011558:Herpesvirus 1, Suid / Q000187:drug effects / Q000254:growth & development*; D051379:Mice; D065108:Ornithine Decarboxylase Inhibitors; D011401:Promoter Regions, Genetic; D011868:Radioisotopes; D013095:Spermidine / Q000378:metabolism / Q000494:pharmacology; D013096:Spermine / Q000378:metabolism / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects; D014162:Transfection; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000096:biosynthesis
D015317:Biogenic Polyamines; D004791:Enzyme Inhibitors; D065108:Ornithine Decarboxylase Inhibitors; D011868:Radioisotopes; D014764:Viral Proteins; D013096:Spermine; D013095:Spermidine; D000518:Eflornithine