[Cellulose decomposition under nitrogen deficiency by bacteria isolated from the intestines of phytophagous vertebrates].
A nitrogen-fixing strain identified as Klebsiella pneumonia 402-2 and two endoglucanase-synthesizing Bacillus strains were isolated from the intestines of phytophagous animals. One of the Bacillus strains was identified as Bacillus subtilis GL. Klebsiella pneumoniae 402-2 increased the endoglucanase activity of both Bacillus strains in mixed cultures. The data on the taxonomic position of strains 402-2 and GL and on the nitrogen-fixing capacity of strain 402-2 were confirmed by sequencing and analyzing their 16S rRNA genes and by amplifying the nitrogenase gene nifH.
Ushakova
N A
NA
Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Leninskii pr.33, Moscow, 119071 Russia.
Belov
L P
LP
Varshavskiĭ
A A
AA
Kozlova
A A
AA
Kolganova
T V
TV
Bulygina
E S
ES
Turova
T P
TP
D000818:Animals; D003411:Arvicolinae / Q000382:microbiology*; D001407:Bacillus / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D001412:Bacillus subtilis / Q000145:classification / Q000201:enzymology / Q000235:genetics; D001717:Birds / Q000382:microbiology*; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D018920:Coculture Techniques; D007420:Intestine, Large / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D010802:Phylogeny; D012329:RNA, Bacterial / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D017421:Sequence Analysis
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D002482:Cellulose; D010088:Oxidoreductases; C029130:nitrogenase reductase; D002480:Cellulase; D009584:Nitrogen