In vitro selection of N peptide-binding RNA on a quartz-crystal microbalance.
We report here an in vitro selection of a hairpin loop-RNA bound to N Peptide from bacteriophage lambda on a 27 MHz quartz-crystal microbalance (QCM) to study an interaction between an RNA structure and an RNA-binding peptide. The N Peptide was immobilized on a QCM electrode and specific RNAs binding to the peptide were selected from a GNRNA pentaloop-randomized RNA library with in situ monitoring of selection processes using a QCM. After the 5th round selection, the consensus sequences including a GCGCA loop were obtained from the pentaloop library. This RNA sequence was different from a binding site of a native N Protein (boxB RNA).
Murakawa
Akiko
A
Department of Biomolecular Engineering, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.
Fukusho
Shinobu
S
Furusawa
Hiroyuki
H
Okahata
Yoshio
Y
D001483:Base Sequence; D009690:Nucleic Acid Conformation; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D011791:Quartz; D012313:RNA / Q000378:metabolism*
D010455:Peptides; D011791:Quartz; D012313:RNA