[The construction of two kinds of artificial bone and the comparison of their osteogenesis capability in vivo].
OBJECTIVE
To construct artificial bone with collagen-hydroxyapatite (HA) or collagen-HA-chondroitin sulfate (CS) as the scaffolds, and observe their biological properties.


METHODS
The artificial bones were constructed by attaching recombinant human bone morphogenetic protein 2 (rhBMP-2) on those scaffolds. And then they were embedded into muscles of rats. Every weekend those newly formed bones were taken from muscles for comparing the difference in osteogenetic capability of two kinds of artificial bone in vivo.


RESULTS
Both kinds of artificial bones could induce bone regeneration in muscle. The collagen-HA-CS artificial bone was superior to the collagen-HA artificial in bone-guided degree.


CONCLUSIONS
The CS could promote the form action of new bone and accelerate the bone healing.
Zhang
Qi-qing
QQ
Department of Biomaterials, Institute of Biomedical Engineering, CAMS, PUMC, Tianjin 300192, China. zhangqiq@public.tpt.tj.cn
Zhang
Li-hai
LH
Liu
Ling-rong
LR
Liang
Yi
Y
Ren
Bai-zhi
BZ
Wang
Fu-jun
FJ
D000818:Animals; D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D001861:Bone Regeneration; D018786:Bone Substitutes; D002809:Chondroitin Sulfates; D003094:Collagen; D006882:Hydroxyapatites; D020007:Implants, Experimental; D010006:Osteoblasts / Q000166:cytology; D010012:Osteogenesis / Q000187:drug effects*; D051381:Rats; D016212:Transforming Growth Factor beta
C525718:BMP2 protein, human; D001672:Biocompatible Materials; C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D018786:Bone Substitutes; D006882:Hydroxyapatites; D016212:Transforming Growth Factor beta; D002809:Chondroitin Sulfates; D003094:Collagen