Differences in the expression of lipolytic-related genes in rat white adipose tissues.
We have investigated in vivo whether the gene expression of the beta3-adrenergic receptor (beta3-AR), perilipin A, hormone-sensitive lipase (HSL), and adipocyte lipid-binding protein (ALBP/aP2) is regulated in a site-specific manner. To induce lipolysis and discriminate between short- and long-term modifications, rats were submitted to an experimental fast for one or five days followed or not by refeeding. The mRNA encoding beta3-AR in retroperitoneal adipose tissue (RP) was significantly increased by one and five days of fasting (4-fold) and then lowered by one day of refeeding (2-fold) compared to fed rats. The reverse trend was observed for perilipin A expression in one day fasted rats. HSL mRNA concentrations were significantly induced (2.2-fold) by five days of fasting relative to fed animals and remained high after refeeding. ALBP/aP2, peroxisome proliferator-activated receptor gamma, and CAAT/enhancer binding protein alpha mRNA levels were essentially unaffected by dietary manipulations. Fasting and/or refeeding were similarly ineffective at regulating gene expression in SC. These data provide a molecular basis for regional differences at different steps of the lipolytic process.
Bertile
Fabrice
F
Centre d'Ecologie et Physiologie Energétiques, UPR 9010 CNRS, associé à l'Université Louis Pasteur, 23 rue Becquerel, 67087 2, Strasbourg Cedex, France. Fabarice.Bertile@c-strasbourg.fr
Criscuolo
François
F
Oudart
Hugues
H
Le Maho
Yvon
Y
Raclot
Thierry
T
D000273:Adipose Tissue / Q000033:anatomy & histology / Q000378:metabolism*; D000818:Animals; D001835:Body Weight; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics; D005215:Fasting; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005786:Gene Expression Regulation; D008066:Lipolysis; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D000070778:Perilipin-1; D010750:Phosphoproteins / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D022702:Receptors, Adrenergic, beta-3 / Q000096:biosynthesis / Q000235:genetics; D002787:Sterol Esterase / Q000096:biosynthesis / Q000235:genetics; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics
D002352:Carrier Proteins; C490174:Fabp7 protein, rat; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D000070778:Perilipin-1; D010750:Phosphoproteins; D012333:RNA, Messenger; D022702:Receptors, Adrenergic, beta-3; D014157:Transcription Factors; D002787:Sterol Esterase