[Effect of 6A8 alpha-mannosidase expression on the proliferative response of human B cell line 3D5].
OBJECTIVE
To study the effect of 6A8 alpha-mannosidase expression on the proliferative response of human B cell 3D5.


METHODS
Recombination of adeno-associated virus vector(rAAV) mediated delivery of sense 6A8 DNA or antisense 6A8 DNA into 3D5 cells, and monoclonal antibody 6A8 alpha staining and Con A binding assay for determination of the change of 6A8 alpha-mannosidase expression, MTT assay for proliferation detection of 3D5 cells driven by Staphylococcus aureus crude cell suspension formalin-fixed (SAC), lower molecular weight B cell growth factor (LMW-BCGF), or rIL-6.


RESULTS
The expression of 6A8 alpha-mannosidase was enhanced in sense 6A8-transduced cells and reduced in antisense 6A8-transduced cells. In comparison with the wild type and the mock-transduced cells, the proliferative response of the sense 6A8-transduced cells to SAC stimulation was enhanced (P < 0.05). However, transduction with antisense 6A8 did not affect the response. In addition, transduction with either sense or antisense 6A8 had no effect on proliferation of 3D5 cells induced by LMW-BCGF or IL-6.


CONCLUSIONS
The proliferative response to SAC stimulation was enhanced in the 3D5 cells with enhanced expression of 6A8 alpha-mannosidase. Either enhancement or reduction of 6A8 alpha-mannosidase expression had no effect on proliferation induced by LMW-BCGF or IL-6.
Zhao
F
F
Department of Immunology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China. zhaoft@hotmail.com
Shi
G
G
Li
L
L
Zhu
L
L
D001402:B-Lymphocytes / Q000166:cytology*; D001426:Bacterial Proteins / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects / Q000502:physiology*; D002460:Cell Line; D006801:Humans; D015847:Interleukin-4 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D014162:Transfection; D043323:alpha-Mannosidase / Q000096:biosynthesis* / Q000235:genetics
D001426:Bacterial Proteins; D015850:Interleukin-6; C089436:anticoagulant protein, Streptomyces cinnamoneus; D015847:Interleukin-4; D043323:alpha-Mannosidase