title: [Fine mapping of susceptibility genes loci within chromosome 1 in Chinese Han families with type 2 diabetes].
abstract: OBJECTIVE
  To confirm previous whole-genome scan results of mapping type 2 diabetes susceptibility genes in chromosome 1 in Northern Chinese Han population by conducting a new genome scan with both an enlarged number of type 2 diabetes families and a new set of microsatellite markers.
  
  
  METHODS
  A genome scan method was applied. After multiplexed PCR, electrophoreses, genescan and genotyping analysis, size informations for all loci were obtained, and a further study was done using both parametric and non-parametric linkage analysis to calculate the P-values and Z-values of these loci.
  
  
  RESULTS
  A total of 34 microsatellite markers distributed within 5 regions along chromosome 1 were surveyed, and 12,000 genotypes were screened. Evidence of linkage with diabetes was identified for 8 of the 34 loci (all the P-values of the 8 loci distributed in 3 regions were lower than 0.05, and the highest Z-value was 2.17). Interestingly, all the 5 markers at the P terminal 1p36.3-1p36.23 region, spanning a long range of 16.9 cM, suggested to be linked with the disease. The results of the other two regions were not consistent with the previous ones.
  
  
  CONCLUSIONS
  The study results have confirmed those gained in the previous genome-wide scan. The fact that all 5 loci at the P terminal region displayed linkage with diabetes suggests that more than 1 susceptibility gene may reside in this region.
authors.affiliation: National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
authors.forename: Wei-nan
authors.initials: WN
authors.lastname: Du
authors.forename: Hong-xia
authors.initials: HX
authors.lastname: Sun
authors.forename: Heng
authors.initials: H
authors.lastname: Wang
authors.forename: Bo-qin
authors.initials: BQ
authors.lastname: Qiang
authors.forename: Zhi-jian
authors.initials: ZJ
authors.lastname: Yao
authors.forename: Jun
authors.initials: J
authors.lastname: Gu
authors.forename: Mo-miao
authors.initials: MM
authors.lastname: Xiong
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Zhu
authors.initials: Z
authors.lastname: Chen
authors.forename: Jin
authors.initials: J
authors.lastname: Zuo
authors.forename: Xiu-feng
authors.initials: XF
authors.lastname: Hua
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.forename: Qi
authors.initials: Q
authors.lastname: Sun
authors.forename: Fu-de
authors.initials: FD
authors.lastname: Fang
mesh_terms: D044466:Asian People; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005006:Ethnicity; D008040:Genetic Linkage; D020022:Genetic Predisposition to Disease; D005820:Genetic Testing; D005838:Genotype; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*