Modulation of X-ray-induced apoptosis in human keratinocytes (HaCaT) by 1,25-dihydroxyvitamin D3.
Possible effects of 1,25-dihydoxyvitamin D3 (vitamin D) on ionizing radiation-induced cell damage have been unknown until now. The task of the present study was to analyze, in a human keratinocyte cell line (HaCaT), the effects of a preincubation with vitamin D on the X-ray-induced mRNA expression of different genes related to apoptosis (gene array). The first results show that ionizing radiation leads to a down-regulation of various apoptosis-relevant genes in HaCaT cells pretreated with vitamin D. Therefore it can be speculated that vitamin D could prove to be a promising radioprotective substance.
Meineke
Viktor
V
Institut für Radiobiologie der Bundeswehr, 80937 Munich, Germany. Viktor.Meineke@t-online.de
Pfaffendorf
Carolina
C
Schinn
Michaela
M
Tilgen
Wolfgang
W
Mayerhofer
Artur
A
Dimitrijevic
Nicola
N
van Beuningen
Dirk
D
Reichrath
Jörg
J
D017209:Apoptosis / Q000528:radiation effects*; D002117:Calcitriol / Q000494:pharmacology*; D002478:Cells, Cultured / Q000187:drug effects / Q000528:radiation effects; D015536:Down-Regulation; D020869:Gene Expression Profiling; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000378:metabolism; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D014965:X-Rays
D012333:RNA, Messenger; D011837:Radiation-Protective Agents; D002117:Calcitriol