Nanoparticle as a new gene transferring vector in specific expression gene.
OBJECTIVE
To evaluate the possibility and efficiency of nanoparticle as a new vector in specific gene transference.


METHODS
Nanoparticle-DNA complex was prepared with Poly-dl-lactic-co-glycolic acid (PLGA) bearing anti-sense monocyte chemotactic protein-1 (A-MCP-1), a specific expression gene, and the package efficiency, release progress in vitro, and the size of the complex were determined. The possibility of the new vector was evaluated with genomic DNA PCR by transferring gene into cultured smooth muscle cells (SMC), cationic lipids as a control. For study in vivo, jugular vein-to-artery bypass grafting procedures were performed on 20 New Zealand white rabbits, of which 6 grafts were transferred with nanoparticle-A-MCP-1 (200 microg), 6 with A-MCP-1 (200 microg) by cationic liposome, 4 with LNCX plasmid, and 4 as control. Fourteen days after the grafts were harvested, the expression of A-MCP-1 and its effect on MCP-1 in vein grafts were detected by dot blot, and the morphologic evaluation of grafts was performed.


RESULTS
The package efficiency of the nanoparticle-DNA complex was 0.9%, release progress in vitro lasted 2 weeks, and the size ranged from 150 to 300 nm. SMC genomic DNA PCR showed that A-MCP-1 gene could be successfully transfected into cells by nanoparticle. The study in vivo indicated that A-MCP-1 mRNA was expressed in both local gene delivery groups, nanoparticle and liposome, meanwhile, MCP-1 expression in vein grafts was significantly inhibited and neointimal hyperplasia was notably reduced.


CONCLUSIONS
Nanoparticle can act as a vector to transfect specific gene.
Heng
Guan
G
Department of General Vascular Surgery, Peking Union Medical College Hospital, CAMS & PUMC, Beijing 100730.
Yongjun
Li
L
Yuehong
Zheng
Z
Changwei
Liu
L
Jing
Yang
Y
Cunxian
Song
S
Pengyan
Wang
W
Sanmei
Zhao
Z
Zongli
Wang
W
Mingpeng
She
S
D000818:Animals; D018932:Chemokine CCL2 / Q000096:biosynthesis / Q000235:genetics*; D016373:DNA, Antisense / Q000096:biosynthesis / Q000235:genetics*; D004337:Drug Carriers; D015870:Gene Expression; D015316:Genetic Therapy; D005822:Genetic Vectors; D019344:Lactic Acid; D036103:Nanotechnology; D010316:Particle Size; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D011817:Rabbits; D014162:Transfection
D018932:Chemokine CCL2; D016373:DNA, Antisense; D004337:Drug Carriers; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid