title: Mesenchymal stem cell injection induces cardiac nerve sprouting and increased tenascin expression in a Swine model of myocardial infarction.
abstract: Stem Cell Induces Cardiac Nerve Sprouting.
  
  
  BACKGROUND
  Mesenchymal stem cell (MSC) transplantation is a promising technique to improve cardiac function. Whether MSC can increase cardiac nerve density and contribute to the improved cardiac function is unclear.
  
  
  RESULTS
  Anterior wall myocardial infarction was created in 16 swine. One month later, 6 swine were given MSC and fresh bone marrow (BM) into infarcted myocardium (MSC group). Four swine were given fresh BM only (BM group), and 6 swine were given culture media (MI-only group). The swine were sacrificed 95.8 +/- 3.5 days after MI. Six normal swine were used as control. Immunocytochemical staining was performed using antibodies against growth-associated protein 43 (GAP43), tyrosine hydroxylase (TH), and three subtypes of tenascin (R, C, and X). Five fields per slide were counted for nerve density. The results show the following. (1). There were more GAP43-positive nerves in the MSC group than in the BM, MI-only, or Control group (P < 0.0001). TH staining showed higher nerve densities in the MSC group than in the MI-only (P < 0.01) or Control group (P < 0.0001) in the atria. (2). There were more sympathetic (TH-positive) nerves in myocardium distant from infarct than in the peri-infarct area (P < 0.05). (3). Optical intensity and color analyses showed significantly higher tenascin R and tenascin C expression in the MSC and BM groups than in the MI-only or Control group (P < 0.01).
  
  
  CONCLUSIONS
  MSC injected with BM into swine infarct results in overexpression of cardiac tenascin, increased the magnitude of cardiac nerve sprouting in both atria and ventricles, and increased the magnitude of atrial sympathetic hyperinnervation 2 months after injection.
authors.affiliation: Department of Medicine, Cedars-Sinai Medical Center and UCLA School of Medicine, Los Angeles, California, USA.
authors.forename: Hui-Nam
authors.initials: HN
authors.lastname: Pak
authors.forename: Mohammed
authors.initials: M
authors.lastname: Qayyum
authors.forename: Dave T
authors.initials: DT
authors.lastname: Kim
authors.forename: Akira
authors.initials: A
authors.lastname: Hamabe
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Miyauchi
authors.forename: Michael C
authors.initials: MC
authors.lastname: Lill
authors.forename: Malkar
authors.initials: M
authors.lastname: Frantzen
authors.forename: Kaname
authors.initials: K
authors.lastname: Takizawa
authors.forename: Lan S
authors.initials: LS
authors.lastname: Chen
authors.forename: Michael C
authors.initials: MC
authors.lastname: Fishbein
authors.forename: Behrooz G
authors.initials: BG
authors.lastname: Sharifi
authors.forename: Peng-Sheng
authors.initials: PS
authors.lastname: Chen
authors.forename: Raj
authors.initials: R
authors.lastname: Makkar
mesh_terms: D000818:Animals; D006348:Cardiac Surgical Procedures / Q000379:methods; D006321:Heart / Q000254:growth & development* / Q000294:innervation*; D006325:Heart Atria / Q000294:innervation / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D006352:Heart Ventricles / Q000294:innervation / Q000378:metabolism / Q000473:pathology; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D023421:Models, Animal; D009203:Myocardial Infarction / Q000378:metabolism* / Q000473:pathology / Q000601:surgery*; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D009416:Nerve Regeneration; D013552:Swine; D013564:Sympathetic Nervous System / Q000254:growth & development* / Q000473:pathology; D019063:Tenascin / Q000378:metabolism*
chemical_list: D019063:Tenascin