DNA topology: topological transformation of DNA by reaction of cisplatin-DNA-core histone complexes with human DNA topoisomerase.
Cis-diamminedichloroplatinium(II), an active antitumor agent binds to core-histone -SV40 DNA complexes prepared by reaction of DNA with core-histone, and alters the fiber-like structure into the loosened structure. In order to model the cisplatin-modified chromatin complexes in cell, the complexes were reacted with human DNA topo II. We found the generations of unique topologically isomers such as trefoil knot (and catenane) and pseudo catenane (and pseudo knot) by reaction of cis-DDP--linearDNA--core-histone complexes with DNA topo I. The results are discussed in relation with a possible recombinational role of topo II (or topo I) on the reaction with cis-DDP--DNA--core-histone complexes.
Kobayashi
Shigeki
S
Department of Analytical Chemistry of Medicines, Showa Pharmaceutical University, 3-3165 Higashi-tamagawagakuen, Machida, Tokyo 194-8543, Japan.
Nakagawa
Sayaka
S
Uehara
Natsuko
N
Hamashima
Hajime
H
Tanaka
Akira
A
D002945:Cisplatin / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004250:DNA Topoisomerases, Type II / Q000737:chemistry*; D006657:Histones / Q000737:chemistry*; D006801:Humans; D011487:Protein Conformation
D006657:Histones; D004247:DNA; D004250:DNA Topoisomerases, Type II; D002945:Cisplatin