[A-Factor as a selective agent for isolation of the soil Gram-negative bacterium strain--the producent of an antibacterial antibiotic].
It was shown the stimulating role of A-factor on soil prokaryotes growth. Soil sample culturing on agar medium, containing A-factor, resulted in the colony forming units (CFU) increasing in comparison with culturing on the medium without this regulator. Gram-negative bacteria were the reason of CFU increasing; previously the effect of A-factor on bacteria of this group was not shown. Gram-negative rod bacterial strain No. 35 was isolated and shown that CFU number was approximately twice increased at A-factor concentration in agar medium 2 and 7 mcg/ml; high A-factor concentration up to 28 mcg/ml was not effective. Isolated strain No. 35 is the producer of antibiotic, effective against gram-positive bacteria.
Gruzina
V D
VD
G. F. Gause Institute of New Antibiotics, Russian Academy of Medical Sciences, Moscow.
Efremenkova
O V
OV
Zenkova
V A
VA
Reznikova
M I
MI
Dudnik
Iu V
IuV
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000378:metabolism* / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D015169:Colony Count, Microbial; D003470:Culture Media; D017753:Ecosystem; D006090:Gram-Negative Bacteria / Q000302:isolation & purification* / Q000378:metabolism; D006094:Gram-Positive Bacteria / Q000254:growth & development; D006133:Growth Substances / Q000378:metabolism* / Q000494:pharmacology; D012988:Soil Microbiology
D000900:Anti-Bacterial Agents; D003470:Culture Media; D006133:Growth Substances; C025035:A-factor (Streptomyces); D015107:4-Butyrolactone