title: Systematic characterization of Escherichia coli genes/ORFs affecting biofilm formation.
abstract: To understand the nature and function of bacterial biofilm and the process of its formation, we have performed systematic screening of a complete set of Escherichia coli genes/open reading frames (ORFs) to identify those that affect biofilm development upon over-expression. In contrast to the biofilm of strain AG1 used as a control, some of the genes/ORFs when over-expressed led to the formation of an abnormal biofilm such as thin, mat-like, filamentous or one easily detaching from various surfaces. Disruptants of selected genes were constructed in order to clarify their roles in the different stages of biofilm formation. Our results suggest that diverse metabolic pathways contribute to the development of biofilm.
authors.affiliation: Graduate School of Science and Technology, Kobe University, Kobe, Japan.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Tenorio
authors.forename: Takashi
authors.initials: T
authors.lastname: Saeki
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Fujita
authors.forename: Madoka
authors.initials: M
authors.lastname: Kitakawa
authors.forename: Tomoya
authors.initials: T
authors.lastname: Baba
authors.forename: Hirotada
authors.initials: H
authors.lastname: Mori
authors.forename: Katsumi
authors.initials: K
authors.lastname: Isono
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D018441:Biofilms / Q000254:growth & development*; D004926:Escherichia coli / Q000235:genetics* / Q000502:physiology*; D010861:Fimbriae, Bacterial / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D016296:Mutagenesis; D016366:Open Reading Frames; D010641:Phenotype
chemical_list: D001425:Bacterial Outer Membrane Proteins