title: [Uptake of drug-containing nanoparticles in dog catotid and femoral arteries].
abstract: OBJECTIVE
  To study the uptake of drug-containing nanoparticles in artery for local therapy of restenosis.
  
  
  METHODS
  Polylactic polyglycolic acid copolymer (PLGA) nanoparticles containing an antiproliferative agent 2-amino-chromone were formulated with an oil-in-water sonication emulsion/solvent evaporation technique. Arterial uptake of nanoparticles was assessed both in vitro and in vivo models of dog and rat carotid and femoral arteries. Epoxide, cyanoacrylate, fibrinogen, fibronectin, didodecyldimethylammonium bromide (DMAB), L-alpha-phosphatidylethanolaine, and lipofectin were selected to modify the surface of nanoparticles to enhance arterial uptake of nanoparticles.
  
  
  RESULTS
  The nanoparticle size ranged from 100 nm to 200 nm, the drug loading in nanoparticles was about 15%, the nanoparticle morphology was observed by scanning electron microscopy and showed spherical shape with smooth surface. Arterial uptake of nanoparticles was enhanced greatly by surface modified nanoparticle with positively charged active agent DMAB. Once nanoparticles located in artery, a high drug level could be maintained at local site for 2 days in vivo model.
  
  
  CONCLUSIONS
  The primary results of animal experiments suggested that nanoparticle could be used as drug carrier of local drug delivery for treating cardiovascular diseases.
authors.affiliation: Department of Polymers, Institute of Biomedical Engineering, CAMS and PUMC, Tianjin 300192, China. cxian@public.tpt.tj.cn
authors.forename: C
authors.initials: C
authors.lastname: Song
authors.forename: Z
authors.initials: Z
authors.lastname: Zhu
authors.forename: J
authors.initials: J
authors.lastname: Yang
authors.forename: H
authors.initials: H
authors.lastname: Sun
authors.forename: X
authors.initials: X
authors.lastname: Leng
authors.forename: L
authors.initials: L
authors.lastname: Wu
authors.forename: L
authors.initials: L
authors.lastname: Tang
authors.forename: P
authors.initials: P
authors.lastname: Wang
authors.forename: R
authors.initials: R
authors.lastname: Levy
mesh_terms: D000818:Animals; D001673:Biodegradation, Environmental; D002339:Carotid Arteries / Q000378:metabolism*; D002867:Chromones; D004285:Dogs; D004337:Drug Carriers; D005263:Femoral Artery / Q000378:metabolism*; D019344:Lactic Acid / Q000737:chemistry / Q000378:metabolism; D008863:Microspheres; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry / Q000378:metabolism; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D013499:Surface Properties
chemical_list: D002867:Chromones; D004337:Drug Carriers; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid