Two-terpyridine.Cu(II) complexes-containing antisense systems for rapid and highly site-specific RNA cleavage.
In an approach toward artificial ribonucleases, novel RNA cleaving systems were constructed that contained two terpyridine.Cu(II) residues. The first antisense system used tandem Cu(II) complex--2'-O-methyloligonucleotide 5'- and 3'-conjugates to cleave an RNA substrate. The second system, which will be described in a future paper, contained two contiguous Cu(II) complex residues at an internal site of a 2'-O-methyloligonucleotide. We found that the first system rapidly cleaved RNA with high site-specificity. Based on these results, we expect the second system to also show efficient RNA cleavage.
Inoue
H
H
Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan.
Furukawa
T
T
Tamura
T
T
Komatsu
Y
Y
Ohtsuka
E
E
D001483:Base Sequence; D001665:Binding Sites; D003300:Copper / Q000737:chemistry; D015195:Drug Design; D066298:In Vitro Techniques; D015394:Molecular Structure; D020320:Oligoribonucleotides, Antisense / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011725:Pyridines / Q000737:chemistry*; D012313:RNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D012260:Ribonucleases / Q000737:chemistry / Q000378:metabolism
D020320:Oligoribonucleotides, Antisense; D011725:Pyridines; D012313:RNA; D003300:Copper; D012260:Ribonucleases