title: Integrated biomonitoring of dioxin-like compounds for waste management and environment.
abstract: Many of the biological and toxic responses of polychlorinated dioxins and furans (PCDD/Fs) have been shown to be mediated through the aryl hydrocarbon receptor (AhR). Several bioanalytical methods have been developed to analyze Ah receptor agonists in cost-and-time effective way such as CALUX-bioassay (chemical-activated luciferase gene expression). This bioassay can detect the sum of dioxin-like activity (including unknown substances) in complex waste and environmental samples. The present paper gives a description of the bioanalytical method and application examples to analyze dioxin-like compounds in waste management samples of thermal/chemical dechlorination processes. The current detection limit of the CALUX in rat hepatoma H4IIE recombinant cells is less than 1 pM TEQ (0.06 pg TEQ/well). The CALUX bioassay is considered to be useful tools for the rapid and integrated monitoring of environmental emission and residual samples as a pre-screening or in combination to chemical analysis.
authors.affiliation: National Institute for Environmental Studies, Research Center for Material Cycles and Waste Management, Onogawa 16-2, Tsukuba, Ibaraki 305-8506, Japan.
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Sakai
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Takigami
mesh_terms: D000818:Animals; D001681:Biological Assay; D004147:Dioxins / Q000032:analysis*; D004784:Environmental Monitoring / Q000379:methods*; D004785:Environmental Pollutants / Q000032:analysis*; D017745:Incineration; D007564:Japan; D051381:Rats; D012680:Sensitivity and Specificity; D018505:Waste Management
chemical_list: D004147:Dioxins; D004785:Environmental Pollutants