title: Fluorescent probes for bacterial cytoplasmic membrane research.
abstract: Fluorescent methods in biological and medical research are extremely useful at the cellular and molecular levels. This is due to sensitive and affordable detection equipment and a variety of specific and more general fluorescent probes, and analytical procedures. In this article, I examine the use of fluorescence membrane probes to study the fluidity (membrane polarization) of the bacterial cytoplasmic membrane, central to energy transduction, ion and nutrient transport and diffusion of water and gases.
authors.affiliation: Department of Environmental Biology, University of Guelph, Guelph, ON N1G 2W1, Canada. jtrevors@uoguelph.ca
authors.forename: J T
authors.initials: JT
authors.lastname: Trevors
mesh_terms: D001419:Bacteria / Q000378:metabolism* / Q000648:ultrastructure*; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure*; D015169:Colony Count, Microbial / Q000379:methods; D005454:Fluorescence Polarization / Q000379:methods*; D005456:Fluorescent Dyes; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D008563:Membrane Lipids