title: Expression of L-CCR in HEK 293 cells reveals functional responses to CCL2, CCL5, CCL7, and CCL8.
abstract: It has become clear in the past years that chemokines and chemokine receptors are pivotal regulators of cellular communication and trafficking. In addition to the approximately 20 chemokine receptors that have been cloned and described, various orphan receptors with a chemokine receptor-like structure are known. We have investigated the orphan mouse chemokine receptor (L-CCR) in HEK 293 cells, a receptor that was originally described in a mouse macrophage cell line. Cells expressing this receptor show pertussis toxin-sensitive chemotaxis and small intracellular calcium transients in response to the chemokines CCL2, CCL7, CCL8, and CCL5. Biotinylated CCL2 binds to L-CCR-expressing cells, and transfection experiments with an L-CCR-green fluorescent protein fusion protein showed L-CCR expression in the membranes of recombinant HEK 293 cells. Although radioligand binding was not detected, it is suggested that L-CCR is a functional chemokine receptor.
authors.affiliation: Department of Medical Physiology, University of Groningen, The Netherlands. K.Biber@med.rug.nl
authors.forename: Knut
authors.initials: K
authors.lastname: Biber
authors.forename: Mike W
authors.initials: MW
authors.lastname: Zuurman
authors.forename: Han
authors.initials: H
authors.lastname: Homan
authors.forename: Hendrikus W G M
authors.initials: HW
authors.lastname: Boddeke
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D019921:Biotinylation; D016466:CHO Cells / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured / Q000378:metabolism; D019742:Chemokines, CC / Q000494:pharmacology*; D002633:Chemotaxis / Q000187:drug effects; D006224:Cricetinae; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D049452:Green Fluorescent Proteins; D007668:Kidney / Q000166:cytology; D008164:Luminescent Proteins / Q000378:metabolism; D051379:Mice; D037342:Pertussis Toxin / Q000494:pharmacology; D010957:Plasmids; D012333:RNA, Messenger / Q000378:metabolism; D054388:Receptors, CCR; D019707:Receptors, Chemokine / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D014018:Tissue Distribution; D014162:Transfection
chemical_list: D000199:Actins; C111851:Ccrl2 protein, mouse; D019742:Chemokines, CC; D008164:Luminescent Proteins; D012333:RNA, Messenger; D054388:Receptors, CCR; D019707:Receptors, Chemokine; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D037342:Pertussis Toxin; D002118:Calcium