[Echinococcus granulosus genotyping by PCR-RFLP].
A method to assess the length polymorphism of restriction fragments of polymerase chain reaction (PCR-LPRF) has been developed and used for the genomic typing of E. granulosus. A scheme optimal under our conditions has been chosen for analysis. To test the chosen scheme, 10 E. granulosus samples from our collection made in Russia and Kazakhstan were examined. The material was obtained from 5 sheep, 1 dog, 2 cows, and 1 man. The E. granulosus genotypes detected in Russia and Kazakhstan do not differ from those described earlier.
Nikulina
N A
NA
Benediktov
I I
II
Garaev
M M
MM
D000818:Animals; D002417:Cattle / Q000469:parasitology; D018509:DNA, Helminth / Q000032:analysis; D004285:Dogs / Q000469:parasitology; D004443:Echinococcosis / Q000469:parasitology / Q000662:veterinary; D004446:Echinococcus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D005838:Genotype; D006801:Humans; D007623:Kazakhstan; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D012426:Russia; D012756:Sheep / Q000469:parasitology; D013552:Swine / Q000469:parasitology
D018509:DNA, Helminth