[A simple method to identify pathogenic fungi in Sabouraud's glucose agar slant culture using fungi-tape and MycoPerm-Blue].
Pathogenic fungi can be identified by growth in special culture medium, colony appearance based on microscopic examination of conidia and hyphae, and by molecular biological techniques. However, these methods are time-consuming and labor-intensive. We describe a simple method for identification of pathogenic fungi from Sabouraud's glucose agar slant culture, using Fungi-Tape and MycoPerm-Blue. It is rapid and easy, and permits visualization of fungal morphology. Using the Fungi-Tape and MycoPerm-Blue technique, we correctly identified 47 of 66 samples, for a 73.4% concordance with conventional identification methods. It is also noteworthy that slides prepared using this method can be stored for long periods without deterioration.
Ogasawara
Yumie
Y
Department of Dermatology and Biomolecular Recognition,Yamaguchi University School of Medicine,1-1-1 Minami-Kogushi, Ube, Yamaguchi, 755-8505 Japan.
Hiruma
Masataro
M
Muto
Masahiko
M
D003470:Culture Media; D005658:Fungi / Q000302:isolation & purification*; D008828:Microbiological Techniques
D003470:Culture Media