title: CREB is required for acquisition of ischemic tolerance in gerbil hippocampal CA1 region.
abstract: Ischemic tolerance is well known as a neuroprotective effect of pre-conditioning ischemia against delayed neuronal death, however, the mechanism or mechanisms underlying this effect are not fully understood. We investigated the relationship between CREB and ischemic tolerance in gerbil hippocampal CA1 neurons using CRE decoy oligonucleotide. Sublethal ischemia led to an increase in the level of CREB phosphorylation in CA1 regions while lethal ischemia did not. Experiments with NG108-15 cells showed that adding CRE decoy oligonucleotide to culture media significantly inhibited the cell growth rate. The administration of CRE decoy oligonucleotide into gerbil cerebral ventricle decreased CREB-DNA binding activity to 38% of the control. Pre-treatment with CRE decoy oligonucleotide 24 h before the induction of ischemic tolerance decreased CA1 neuronal cell survival to 21% of the control. The present findings suggest that a CREB-mediated transcription system is necessary for the induction of ischemic tolerance.
authors.affiliation: Department of Neurosurgery, Kumamoto University School of Medicine, Kumamoto, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Hara
authors.forename: Jun-ichiro
authors.initials: J
authors.lastname: Hamada
authors.forename: Shigetoshi
authors.initials: S
authors.lastname: Yano
authors.forename: Motohiro
authors.initials: M
authors.lastname: Morioka
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Kai
authors.forename: Yukitaka
authors.initials: Y
authors.lastname: Ushio
mesh_terms: D000818:Animals; D001667:Binding, Competitive / Q000187:drug effects; D002545:Brain Ischemia / Q000378:metabolism / Q000503:physiopathology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D017362:Cyclic AMP Response Element-Binding Protein / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D004195:Disease Models, Animal; D005849:Gerbillinae; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism / Q000503:physiopathology*; D006822:Hybrid Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D019194:Ischemic Preconditioning; D008297:Male; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D009841:Oligonucleotides / Q000494:pharmacology; D010766:Phosphorylation; D016548:Prosencephalon / Q000098:blood supply / Q000503:physiopathology; D051381:Rats; D014162:Transfection
chemical_list: D017362:Cyclic AMP Response Element-Binding Protein; D009841:Oligonucleotides; D004247:DNA