[Molecular cloning of Plasmodium yoelii dynamin-like protein (PyDyn) gene and the immunological character of its domains].
OBJECTIVE
To identify and clone a new full ORF gene of PyDyn (Plasmodium yoelii dynamin-like protein), and examine the protection of their expression products.


METHODS
Using the P. yoelii Genome technology and RT-PCR.


RESULTS
The full ORF gene of PyDyn was amplified from mRNA of the erythrocytic stage of P. yoelii., three domains of PyDyn were expressed in E. coli., and the fairly positive immunogenicity of them was showed by IFA. The full ORF gene of PyDyn was 2,433 bp and encode 811 amino acids. Its Gene Bank access number is AF458071. PyDyn belongs to the dynamin-like protein family according to its property.


CONCLUSIONS
The new full ORF gene of PyDyn is obtained and identified; their expressed domains are probably new candidates for malaria vaccine.
Wang
Dong
D
Department of Etiology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China.
Mao
Ying-hong
YH
Wang
Heng
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D034281:Dynamins / Q000235:genetics* / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics; D017125:Genes, Protozoan / Q000235:genetics / Q000276:immunology*; D017780:Malaria Vaccines / Q000276:immunology; D008969:Molecular Sequence Data; D010967:Plasmodium yoelii / Q000737:chemistry / Q000235:genetics*; D015800:Protozoan Proteins / Q000235:genetics* / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology
D017780:Malaria Vaccines; D015800:Protozoan Proteins; D014614:Vaccines, Synthetic; D034281:Dynamins