title: [Alkaloids from a Tibetan medicine Meconopsis quintuplinervia Regel].
abstract: OBJECTIVE
  To reinvestigate the chemical constituents of the ethanolic extract of Meconopsis quintuplinervia Regel which is a traditional Tibetan medicine used for treatments of hepatitis, tuberculosis etc..
  
  
  METHODS
  The compounds were enriched by column chromatography techniques over silica gel, macro porous resin and Sephadex LH-20 absorbents, and finally purified by reverse phase preparative HPLC methods with isocratic mobile phase systems of methanol-H2O-acetic acid (500:500:1) and acetonitrile-H2O-acetic acid (200:800:1). Structural determination of the pure compounds were based on extensive analyses of modern spectroscopic methods including IR, MS, HRMS, 1D- and 2D-NMR spectra.
  
  
  RESULTS
  Three alkaloids were obtained and their structures were elucidated as norsanguinarine (I), O-methylflavinantine (II) and 6-methoxy-17-methyl-2, 3-[methylenebis (oxy)]-morphin-5-en-7-one (III).
  
  
  CONCLUSIONS
  Norsanguinarine (I) was isolated from genus Meconopsis for the first time, and 6-methoxy-17-methyl-2,3-[methylenebis(oxy)]-morphin-5-en-7-one (III) is a new alkaloid named as meconoquintupline.
authors.affiliation: Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
authors.forename: Xiao-ya
authors.initials: XY
authors.lastname: Shang
authors.forename: Jian-gong
authors.initials: JG
authors.lastname: Shi
authors.forename: Yong-chun
authors.initials: YC
authors.lastname: Yang
authors.forename: Xing
authors.initials: X
authors.lastname: Liu
authors.forename: Chong
authors.initials: C
authors.lastname: Li
authors.forename: Cheng-zhong
authors.initials: CZ
authors.lastname: Zhang
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification; D038861:Medicine, Tibetan Traditional; D008968:Molecular Conformation; D015394:Molecular Structure; D009019:Morphinans / Q000737:chemistry / Q000302:isolation & purification; D009022:Morphine Derivatives / Q000737:chemistry / Q000302:isolation & purification*; D028101:Papaveraceae / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: C483649:6-methoxy-17-methyl-2,3-(methylenebis(oxy))-morphin-5-en-7-one; D000470:Alkaloids; D009019:Morphinans; D009022:Morphine Derivatives; C483650:norsanguinarine; C024736:2-methylflavinantine