Humoral and cell-mediated immune responses of d/d histocompatible pigs against classical swine fever (CSF) virus.
A better understanding of cell-mediated immune responses to classical swine fever virus (CSFV) is essential for the future development of improved vaccines. We analyzed the generation of cell-mediated and humoral immune responses in d/d histocompatible pigs following CSFV infection or vaccination. Viral infection induced high T cell responses with high primary and secondary CTL activity correlated with high IFN-gamma production, whereas vaccination with a live vaccine followed by infection mainly induced neutralizing antibody but low cell-mediated responses. Moreover, high IgG1 response was associated with high IFN-gamma response following infection whereas a weak IFN-gamma response was related to a good IgG2 response but a low IgG1 production. These data could reflect Th1/Th2-like balance of immune responses depending upon immunization protocols, which has not yet been described in the pig. T-cell responses to CSFV were evidenced by CSFV-specific CD25 upregulation on CD4-CD8+, but not on CD4+CD8- cells, which further illustrated the importance of CTL responses after infection. Our results indicated that generation of cell-mediated immune responses was much higher following intranasal/oral CSFV infection than after intramuscular vaccination, which implies that the capacity of new CSFV vaccines to induce higher T-cell responses should be considered.
Piriou
Laurence
L
Agence Française de Sécurité Sanitaire des Aliments, Unité de Virologie et Immunologie Porcines, BP 53, 22440 Ploufragan, France.
Chevallier
Sylvie
S
Hutet
Evelyne
E
Charley
Bernard
B
Le Potier
Marie-Frédérique
MF
Albina
Emmanuel
E
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000097:blood*; D006691:Classical Swine Fever / Q000276:immunology* / Q000821:virology; D006692:Classical Swine Fever Virus / Q000235:genetics / Q000276:immunology*; D007111:Immunity, Cellular / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics; D007700:Kinetics; D008213:Lymphocyte Activation; D009500:Neutralization Tests / Q000662:veterinary; D012367:RNA, Viral / Q000097:blood; D013552:Swine; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014611:Vaccination / Q000662:veterinary; D014765:Viral Vaccines / Q000276:immunology
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D012367:RNA, Viral; D014765:Viral Vaccines; D007371:Interferon-gamma