A potential vector of domoic acid: the swimming crab Polybius henslowii Leach (Decapoda-brachyura).
The swimming crab Polybius henslowii may play an important role in the movement of the amnesic shellfish toxin, domoic acid (DA), through the marine food chain. High DA concentrations have been determined in crab samples harvested along the Portuguese coast during the summer of 2002, reaching a level of 323.1 microg DA/g crab tissue. Toxin distribution in the different crab organs showed levels as high as 571.6 microg DA/g in the visceral tissues. Levels of toxin 4-12 times lower were detected in the remaining tissues. This crab might be a prominent vector of the toxin to higher trophic levels, including fishes, sea birds and even humans. In Portugal P. henslowii is commercialised during the summer in some local markets. DA concentrations were found close to the legal limit of 20 microg/g in samples purchased at Figueira da Foz market. The crabs are boiled prior to reaching the consumers. The cooking process was evaluated. Determination of toxin losses during the cooking process showed a toxin reduction higher than 50%. DA was determined by HPLC-UV and confirmed by spectra acquired with diode-array detector.
Costa
Pedro R
PR
Departamento de Ambiente Aquático, Instituto de Investigação das Pescas e do Mar (IPIMAR). Avenida de Brasília, 1449-006 Lisboa, Portugal. prcosta@ipimar.pt
Rodrigues
Susana M
SM
Botelho
Maria J
MJ
Sampayo
Maria Antónia de M
MA
D000818:Animals; D003386:Brachyura / Q000033:anatomy & histology / Q000737:chemistry*; D003296:Cooking; D004777:Environment; D020387:Food Chain; D006801:Humans; D007608:Kainic Acid / Q000031:analogs & derivatives* / Q000032:analysis* / Q000633:toxicity; D008387:Marine Toxins / Q000032:analysis / Q000633:toxicity; D011174:Portugal; D012621:Seasons; D012758:Shellfish / Q000633:toxicity*; D013056:Spectrophotometry, Ultraviolet
D008387:Marine Toxins; C012301:domoic acid; D007608:Kainic Acid