title: [Fragile X mental retardation protein interacts with human NDK/Nm23-H2].
abstract: OBJECTIVE
  To investigate the physiological role of fragile X mental retardation protein (FMRP) and screen the proteins interacting with FMRP in human fetal hippocampus cDNA library.
  
  
  METHODS
  Human fetal hippocampus cDNA library was constructed in yeast two-hybrid DAD vector pGAD10. Quality of the library was measured by picking up random colonies as templates for PCR testing. Proteins interacting with FMRP were screened by yeast two-hybrid system. Furthermore, the interaction site of FMRP was mapped in yeast.
  
  
  RESULTS
  The average length of inserts of the two-hybrid library was 1.5 kb, and the ratio of recombinant colonies was about 90%. Human NDK/Nm23-H2 was found interacting with FMRP. NDK/Nm23-H2 interacted with FMRP exon 1-12, as well as FMRP isoforms without exon 12, and exons 14-17. NDK/Nm23-H2 couldn't interact with FMRP exon 1-6 and exon 2-7 fragments.
  
  
  CONCLUSIONS
  Human NDK/Nm23-H2 can bind FMRP directly. The interaction site of FMRP is located at its exon 1-11. This interaction in vitro might alter the intracellular distribution of NDK/Nm23-H2, and even regulates the transcription and expression of FMRP.
authors.affiliation: National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China.
authors.forename: R
authors.initials: R
authors.lastname: Lou
authors.forename: P C
authors.initials: PC
authors.lastname: Mei
authors.forename: J Y
authors.initials: JY
authors.lastname: Gong
authors.forename: N
authors.initials: N
authors.lastname: Zhu
authors.forename: Z H
authors.initials: ZH
authors.lastname: Kou
authors.forename: G Y
authors.initials: GY
authors.lastname: Wu
authors.forename: Y
authors.initials: Y
authors.lastname: Shen
mesh_terms: D051860:Fragile X Messenger Ribonucleoprotein 1; D005600:Fragile X Syndrome / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D054778:NM23 Nucleoside Diphosphate Kinases; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009701:Nucleoside-Diphosphate Kinase / Q000378:metabolism*; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D051860:Fragile X Messenger Ribonucleoprotein 1; D054778:NM23 Nucleoside Diphosphate Kinases; D009419:Nerve Tissue Proteins; D009701:Nucleoside-Diphosphate Kinase; D011506:Proteins; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; C497683:FMR1 protein, human; C517938:NME1 protein, human