title: A new strategy to determine the target DNA sequence of a short peptide.
abstract: The design of molecules that target desired DNA sequences has been one of the major challenges in the field of molecular recognition. We report here a general strategy for defining the sequence-preference of DNA-binding short peptide by using its heterodimer. Our method successfully identified specific sequences of short peptides derived from native DNA-binding proteins. The usefulness of this approach has been demonstrated by identifying preferred DNA targets for a peptide composed only of D-amino acids.
authors.affiliation: PRESTO, JST, Kyoto University, Uji, Kyoto 611-0011, Japan.
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Sato
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Honjyo
authors.forename: Shinji
authors.initials: S
authors.lastname: Tsuji
authors.forename: Masaki
authors.initials: M
authors.lastname: Hagihara
authors.forename: Keisuke
authors.initials: K
authors.lastname: Makino
authors.forename: Takashi
authors.initials: T
authors.lastname: Morii
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D024202:Electrophoretic Mobility Shift Assay; D010455:Peptides / Q000737:chemistry*
chemical_list: D010455:Peptides; D004247:DNA