The effects of metabolites from the indigenous Acidithiobacillus thiooxidans and temperature on the bioleaching of cadmium from soil.
The effect of metabolites from the indigenous Acidithiobacillus thiooxidans and temperature on the bioleaching of cadmium from soil was investigated in the present study. Bioleaching was found to be more effective than chemical leaching of cadmium. The metabolite, mainly sulfuric acid, which was shown to be growth-associated in the exponential phase, plays a major role in bioleaching. The maximum amount of cadmium leached was obtained after 8 days of precultivation when cells were directly involved in the leaching process. It indicates that cells in the exponential growth phase exhibit higher activity toward bioleaching. In contrast, the maximum amount of cadmium leached and the maximum initial rate for bioleaching were reached after 16 days of precultivation when only metabolites were involved in the bioleaching process. It implies that higher sulfuric acid concentration results in higher leaching efficiency. In addition, higher temperature leads to higher leaching efficiency. The optimal operation condition for bioleaching was determined to be a two-stage process: The first stage involves the precultivation of the indigenous A. thiooxidans at 30 degrees C for 8 days followed by 20 minutes of centrifugation to discard cells. The second stage involves the bioleaching with the subsequent supernatant at 50 degrees C.
Liu
Hsuan-Liang
HL
Department of Chemical Engineering, National Taipei University of Technology, No. 1 Sec. 3 Chung-Hsiao E Road, Taipei 10643, Taiwan, Republic of China.
Chiu
Chi-Wei
CW
Cheng
Yang-Chu
YC
D013856:Acidithiobacillus thiooxidans / Q000254:growth & development / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002104:Cadmium / Q000378:metabolism*; D018929:Cell Culture Techniques; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D013464:Sulfuric Acids / Q000737:chemistry / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
D012989:Soil Pollutants; D013464:Sulfuric Acids; D002104:Cadmium; C033158:sulfuric acid