Elimination of false-negative hepatitis C virus RNA results by removal of inhibitors in cadaver-organ donor blood specimens.
Detection of viral nucleic acids in blood samples from cadavers is often difficult because of inhibition of the reverse transcriptase (RT) or polymerase chain reaction (PCR) steps by substances present in the samples. A robust method for the extraction and detection of hepatitis C virus (HCV) RNA from cadaver blood samples by polymerase chain reaction RT-PCR has been developed on the basis of the Qiagen QIAamp DNA mini kit extraction system (Basel, Switzerland). Twenty of 36 samples tested were positive for HCV RNA. Six of the 16 HCV-antibody- and RNA-negative samples contained inhibitors that were successfully removed by pretreatment of samples with the Qiagen AX matrix before extraction.
Padley
David J
DJ
Division of Virology, National Institute for Biological Standards and Control, South Mimms, Herts, United Kingdom.
Lucas
Sebastian B
SB
Saldanha
John
J
D002102:Cadaver; D005188:False Negative Reactions; D016174:Hepacivirus / Q000235:genetics* / Q000302:isolation & purification; D006526:Hepatitis C / Q000097:blood / Q000175:diagnosis*; D006801:Humans; D016377:Organ Transplantation; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D012367:RNA, Viral / Q000097:blood* / Q000302:isolation & purification; D018894:Reverse Transcriptase Inhibitors / Q000097:blood / Q000302:isolation & purification; D014019:Tissue Donors
D012367:RNA, Viral; D018894:Reverse Transcriptase Inhibitors