Development of novel whole-mount in situ hybridization (WISH).
We have designed a novel fluorescent PNA probe which can be rapidly introduced into the living cell without any complicated pretreatment. We could post-synthetically incorporate amino acid derivatives having membrane permeability function into the PNA probe by utilizing key compound 1 which we have already developed. We show that the PNA probe will be a candidate for a rapidly penetrating and non-degrading probe for WISH.
Ikeda
Hisafumi
H
Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan.
Tonosaki
Madoka
M
Sugiyama
Akinori
A
Tashiro
Fumio
F
Nakamura
Yushin
Y
D005456:Fluorescent Dyes; D017403:In Situ Hybridization / Q000379:methods*; D015335:Molecular Probes; D020135:Peptide Nucleic Acids / Q000737:chemistry
D005456:Fluorescent Dyes; D015335:Molecular Probes; D020135:Peptide Nucleic Acids