[Expression of chloride channel in nasal mucosa with allergic rhinitis].
OBJECTIVE
To evaluate the role of chloride channel ClC-3 in perennial allergic rhinitis.


METHODS
The nasal mucosa from 19 patients with perennial allergic rhinitis were studied by immunohistochemistric staining and reverse transcriptase polymerase chain reaction (RT-PCR) for the expression of ClC-3.


RESULTS
ClC-3 was positively stained in 17 cases of 19 patients, with a rate of 89.6%. It mainly distributed in cytoplasm of epithelium and submucosal gland. All tissues displayed expected DNA length after PCR. The relative density of ClC-3 in mucosa with allergic rhinitis was 0.685 +/- 0.114 and 0.140 +/- 0.075 in normal.


CONCLUSIONS
ClC-3 played an important role in the over-secretion of liquor in allergic rhinitis.
Li
Hua-bin
HB
Beijing Institute of Otolaryngology, Department of Otolaryngology Head and Neck Surgery, Tongren Hospital, Capital Medical University, Beijing, 100005, China. lhb7351@hotmail.com
Han
De-min
DM
Zhou
Bing
B
Wang
Xiang-dong
XD
Liu
Zhong-yan
ZY
Yang
Ling
L
D000293:Adolescent; D000328:Adult; D018118:Chloride Channels / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009297:Nasal Mucosa / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012221:Rhinitis, Allergic, Perennial / Q000378:metabolism*
D018118:Chloride Channels; C115376:ClC-3 channel