title: RNA cleavage by a mammalian tRNA 3'processing endoribonuclease (3'tRNase) reduces HIV-1 expression.
abstract: We examined the suppression of HIV-1 expression by cleavage of the HIV-1 RNA gene, using a mammalian tRNA 3'processing endoribonuclease (3'tRNase) as an External Guide Sequence oligozyme (EGS) in vivo. The enzyme can also recognize and cleave a target RNA that forms a pre-tRNA like complex with a substrate RNA. We designed an EGS to specifically recognize the packaging signal region (p.s.) and the upstream portion of the gag region (g.s.) of HIV-1 mRNA, and constructed EGS expression vectors that used the tRNA(met) promoter as an expression cassette for the EGS. Their cleavage activities were assessed in cell culture, using a transient assay system. The EGS efficiencies were determined by co-transfection of the EGS expression vectors and the HIV-1 gene plasmid vector (pNL-luc) into COS cells. The assay results showed a significant inhibition of the HIV-1 gag p24 antigen expression. In addition, we constructed MLV-based VSV-G pseudotyped retrovirus vectors that express the EGS. They were co-infected into MT-4 cells with the HIV-1 virus (NL4-3). These EGS expression retrovirus vectors exhibited a similar inhibitory effect.
authors.affiliation: Department of Industrial Chemistry, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, Japan.
authors.forename: Masakazu
authors.initials: M
authors.lastname: Yukita
authors.forename: Michiko
authors.initials: M
authors.lastname: Kitano
authors.forename: Naoko
authors.initials: N
authors.lastname: Miyano-Kurosaki
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Takeuchi
authors.forename: Masayuki
authors.initials: M
authors.lastname: Nashimoto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takaku
mesh_terms: D000818:Animals; D001483:Base Sequence; D004722:Endoribonucleases / Q000378:metabolism*; D015497:HIV-1 / Q000235:genetics*; D006868:Hydrolysis; D009690:Nucleic Acid Conformation; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism*; D012367:RNA, Viral / Q000737:chemistry / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral; D004722:Endoribonucleases; C045782:3'-pre-tRNase