Identification and characterization of 2 types of erythroid progenitors that express GATA-1 at distinct levels.
Transcription factor GATA-1 is essential for the development of the erythroid lineage. To ascertain whether strict control of GATA-1 expression level is necessary for achieving proper erythropoiesis, we established transgenic mouse lines expressing green fluorescent protein (GFP) under the control of the GATA-1 gene hematopoietic regulatory domain. We examined the GATA-1 expression level by exploiting the transgenic mice and found 2 GFP-positive hematopoietic progenitor fractions in the bone marrow. One is the GFPhigh fraction containing mainly CFU-E and proerythroblasts, which coexpress transferrin receptor, while the other is the GFPlow/transferrin receptor-negative fraction containing BFU-E. Since the intensity of green fluorescence correlates well with the expression level of GATA-1, these results indicate that GATA-1 is highly expressed in erythroid colony-forming unit (CFU-E) but low in erythroid burst-forming unit (BFU-E), suggesting that the incremental expression of GATA-1 is required for the formation of erythroid progenitors. We also examined GFP-positive fractions in the transgenic mouse spleen and fetal liver and identified fractions containing BFU-E and CFU-E, respectively. This study also presents an efficient method for enriching the CFU-E and BFU-E from mouse hematopoietic tissues.
Suzuki
Norio
N
Center for Tsukuba Advanced Research Alliance, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba 305-8577, Japan.
Suwabe
Naruyoshi
N
Ohneda
Osamu
O
Obara
Naoshi
N
Imagawa
Shigehiko
S
Pan
Xiaoqing
X
Motohashi
Hozumi
H
Yamamoto
Masayuki
M
D000818:Animals; D001854:Bone Marrow Cells; D002118:Calcium / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000032:analysis / Q000096:biosynthesis*; D015672:Erythroid Precursor Cells / Q000166:cytology* / Q000378:metabolism; D050981:Erythroid-Specific DNA-Binding Factors; D004920:Erythropoiesis; D050982:GATA1 Transcription Factor; D005786:Gene Expression Regulation; D049452:Green Fluorescent Proteins; D016130:Immunophenotyping; D008099:Liver / Q000166:cytology; D008164:Luminescent Proteins / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D011990:Receptors, Transferrin / Q000032:analysis; D013154:Spleen / Q000166:cytology; D014157:Transcription Factors / Q000032:analysis / Q000096:biosynthesis*
D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D050982:GATA1 Transcription Factor; C494689:Gata1 protein, mouse; D008164:Luminescent Proteins; D011990:Receptors, Transferrin; D014157:Transcription Factors; D049452:Green Fluorescent Proteins; D002118:Calcium