title: Hypoalbuminaemia enhances the renal vasoconstrictor effect of lysophosphatidylcholine.
abstract: BACKGROUND
  Lysophosphatidylcholine (LPC) causes vascular dysfunction in vitro. Lipoprotein LPC is increased in hypoalbuminaemia. Albumin binds LPC and restores LPC-induced abnormalities. We hypothesized that in vivo LPC impairs blood flow more in hypoalbuminaemia than in normoalbuminaemia.
  
  
  METHODS
  Increasing concentrations of LPC were infused intra-renally in Nagase analbuminaemic rats (NAR) and Sprague-Dawley rats (controls).
  
  
  RESULTS
  Intra-renal LPC (0.1 micromol/min, 20 min) reduced renal blood flow (RBF) more (P < 0.01) in NAR (from 8.3 +/- 0.3 to 4.0 +/- 1.1) than in controls (from 7.7 +/- 0.7 to 5.8 +/- 0.5 ml/min/g kidney). Lysophosphatidylethanolamine had no effect. After stopping LPC, RBF recovery was delayed in NAR [median 90 (range: 70-90) vs 45 min (40-60), P < 0.01]. Intravenous bovine serum albumin (BSA) prevented LPC-induced vasoconstriction in both strains. Prolonging LPC for 60 min delayed recovery of RBF. In this setting, intra-renal BSA completely restored RBF in 75 min (30-90), while intra-renal saline over 75 min only resulted in 33 +/- 13% recovery (P < 0.01). Baseline renal LPC content was unchanged in NAR. However, intra-renal LPC infusion doubled renal LPC content in NAR, but had no effect in controls.
  
  
  CONCLUSIONS
  In NAR, baseline RBF and renal LPC content are normal. However, exposure of NAR to LPC results in much more vasoconstriction and accumulation of LPC than in normoalbuminaemia. Addition of albumin prevents and restores LPC-induced vasoconstriction.
authors.affiliation: Department of Nephrology and Hypertension, University Medical Center, Utrecht, The Netherlands.
authors.forename: Thi Danh
authors.initials: TD
authors.lastname: Vuong
authors.forename: Branko
authors.initials: B
authors.lastname: Braam
authors.forename: Nel
authors.initials: N
authors.lastname: Willekes-Koolschijn
authors.forename: Peter
authors.initials: P
authors.lastname: Boer
authors.forename: Hein A
authors.initials: HA
authors.lastname: Koomans
authors.forename: Jaap A
authors.initials: JA
authors.lastname: Joles
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D034141:Hypoalbuminemia / Q000503:physiopathology*; D007668:Kidney / Q000187:drug effects / Q000503:physiopathology*; D008244:Lysophosphatidylcholines / Q000494:pharmacology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D017207:Rats, Sprague-Dawley; D012039:Regional Blood Flow / Q000187:drug effects; D014662:Vasoconstrictor Agents / Q000494:pharmacology*
chemical_list: D008244:Lysophosphatidylcholines; D014662:Vasoconstrictor Agents