Effect of adhesion or collagen molecules on cell attachment, insulin secretion, and glucose responsiveness in the cultured adult porcine endocrine pancreas: a preliminary study.
The effect of either adhesion or collagen molecules on cell attachment, insulin secretion, and glucose responsiveness was investigated in adult porcine pancreatic endocrine (PE) cells that were cultured for a longer term in vitro. Six different types of molecules--laminin, fibronectin, poly-L-lysine (PLL), type I collagen, gelatin, and Matrigel--were used. Approximately 2.0 x 10(5) cells per dish of each molecule type were cultured for 4 weeks. In the laminin group, the insulin accumulation was maintained at a significantly higher level than in the control group at 4 weeks of culture, and glucose-stimulated insulin secretion and the insulin-positive rate were also higher than in the control group. In the Matrigel group, islet-like cell clusters were formed, but insulin accumulation rapidly decreased at 3-4 weeks of culture. A large number of PE cells attached tightly and spread in the fibronectin group until the fourth week of culture, but their function was not better than those in the control group. In the PLL and gelatin groups, the PE cell function was not significantly different from that of the control group. In the type I collagen group, insulin secretion was inferior to that of the other groups. The results of this study suggest that laminin is the most suitable extracellular matrix for the long-term culture preservation of PE cells.
Edamura
Kazuya
K
Division of Cell Replacement and Regenerative Medicine, Medical Research Institute, School of Medicine, Tokyo Women's Medical University, Japan.
Nasu
Koko
K
Iwami
Yukiko
Y
Ogawa
Hiroyuki
H
Sasaki
Nobuo
N
Ohgawara
Hisako
H
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism / Q000494:pharmacology*; D004338:Drug Combinations; D005109:Extracellular Matrix / Q000737:chemistry; D005353:Fibronectins / Q000494:pharmacology; D005780:Gelatin / Q000494:pharmacology; D005947:Glucose / Q000235:genetics / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D007797:Laminin / Q000494:pharmacology; D011107:Polylysine / Q000494:pharmacology; D011509:Proteoglycans / Q000494:pharmacology; D013552:Swine
D004338:Drug Combinations; D005353:Fibronectins; D007328:Insulin; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D011107:Polylysine; D005780:Gelatin; D003094:Collagen; D005947:Glucose