title: A cattle-human comparative map built with cattle BAC-ends and human genome sequence.
abstract: As a step toward the goal of adding the cattle genome to those available for multispecies comparative genome analysis, 40,224 cattle BAC clones were end-sequenced, yielding 60,547 sequences (BAC end sequences, BESs) after trimming with an average read length of 515 bp. Cattle BACs were anchored to the human and mouse genome sequences by BLASTN search, revealing 29.4% and 10.1% significant hits (E < e-5), respectively. More than 60% of all cattle BES hits in both the human and mouse genomes are located within known genes. In order to confirm in silico predictions of orthologyand their relative position on cattle chromosomes, 84 cattle BESs with similarity to sequences on HSA11 were mapped using a cattle-hamster radiation hybrid (RH) panel. Resulting RH maps of BTA15 and BTA29 cover approximately 85% of HSA11 sequence, revealing a complex patchwork shuffling of segments not explained by a simple translocation followed by internal rearrangements. Overlay of the mouse conserved syntenies onto HSA11 revealed that segmental boundaries appear to be conserved in all three species. The BAC clone-based comparative map provides a foundation for the evolutionary analysis of mammalian karyotypes and for sequencing of the cattle genome.
authors.affiliation: Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, Illinois, 61801 USA.
authors.forename: Denis M
authors.initials: DM
authors.lastname: Larkin
authors.forename: Annelie
authors.initials: A
authors.lastname: Everts-van der Wind
authors.forename: Mark
authors.initials: M
authors.lastname: Rebeiz
authors.forename: Peter A
authors.initials: PA
authors.lastname: Schweitzer
authors.forename: Sharon
authors.initials: S
authors.lastname: Bachman
authors.forename: Cheryl
authors.initials: C
authors.lastname: Green
authors.forename: Chris L
authors.initials: CL
authors.lastname: Wright
authors.forename: Edhilvia J
authors.initials: EJ
authors.lastname: Campos
authors.forename: Leslie D
authors.initials: LD
authors.lastname: Benson
authors.forename: Jennifer
authors.initials: J
authors.lastname: Edwards
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Kazutoyo
authors.initials: K
authors.lastname: Osoegawa
authors.forename: James E
authors.initials: JE
authors.lastname: Womack
authors.forename: Pieter J
authors.initials: PJ
authors.lastname: de Jong
authors.forename: Harris A
authors.initials: HA
authors.lastname: Lewin
mesh_terms: D000818:Animals; D002417:Cattle; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D017124:Conserved Sequence; D004269:DNA, Bacterial / Q000032:analysis; D005819:Genetic Markers; D015894:Genome, Human; D006801:Humans; D051379:Mice; D020161:Physical Chromosome Mapping / Q000379:methods; D011237:Predictive Value of Tests; D021101:Radiation Hybrid Mapping / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods; D012984:Software; D026801:Synteny
chemical_list: D004269:DNA, Bacterial; D005819:Genetic Markers