title: Threonyl-tRNA synthetase of archaea: importance of the discriminator base in the aminoacylation of threonine tRNA.
abstract: To investigate the contribution of the discriminator base of archaeal tRNA(Thr) in aminoacylation by threonyl-tRNA synthetase (ThrRS), cross-species aminoacylation between Escherichia coli and Haloferax volcanii, halophilic archaea, was studied. It was found that E. coli ThrRS threonylated the H. volcanii tRNA(Thr) but that E. coli threonine tRNA was not aminoacylated by H. volcanii ThrRS. Results of a threonylation experiment using in vitro mutants of E. coli threonine tRNA showed that only the mutant tRNA(Thr) having U73 was threonylated by H. volcanii ThrRS. These findings indicate that the discriminator base U73 of H. volcanii tRNA(Thr) is a strong determinant for the recognition by ThrRS.
authors.affiliation: Department of Material and Biological Chemistry, Faculty of Science, Yamagata University, Yamagata 990-8560, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ishikura
authors.forename: Y
authors.initials: Y
authors.lastname: Nagaoka
authors.forename: J
authors.initials: J
authors.lastname: Yokozawa
authors.forename: T
authors.initials: T
authors.lastname: Umehara
authors.forename: A
authors.initials: A
authors.lastname: Kuno
authors.forename: T
authors.initials: T
authors.lastname: Hasegawa
mesh_terms: D004926:Escherichia coli / Q000378:metabolism; D019622:Haloferax volcanii / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D019642:RNA, Archaeal / Q000235:genetics / Q000378:metabolism; D012329:RNA, Bacterial / Q000378:metabolism; D012346:RNA, Transfer, Amino Acyl / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity; D013379:Substrate Specificity; D013914:Threonine-tRNA Ligase / Q000378:metabolism*
chemical_list: D019642:RNA, Archaeal; D012329:RNA, Bacterial; D012346:RNA, Transfer, Amino Acyl; D013914:Threonine-tRNA Ligase