title: Isolation of new splice isoforms, characterization and expression analysis of the human septin SEPT8 (KIAA0202).
abstract: SEPT8 (KIAA0202) is a member of the highly conserved septin family. Septins are membrane-associated GTPases which are involved in cytokinesis and cellular morphogenesis. Using the yeast two-hybrid system and the glutathione-S-transferase pull-down assay we previously had identified the SEPT8 (KIAA0202) as interaction partner of the human septin SEPT5 (cell division cycle related-1, CDCrel-1). Since the complete cDNA sequence of the human septin SEPT8 (KIAA0202) was not known at that time, we isolated new 5' and 3' cDNA sequence of SEPT8 (KIAA0202) by screening three different cDNA libraries. In addition, we performed the characterization of SEPT8 (KIAA0202) and identified new splice variants of SEPT8 (KIAA0202). The expression pattern of SEPT8 (KIAA0202) and its interaction partner SEPT5 (CDCrel-1) is illustrated.
authors.affiliation: Department of Pediatrics and Adolescent Medicine, University of Freiburg, Children's Hospital, Mathildenstrasse 1, D-79106 Freiburg, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Bläser
authors.forename: Katrin
authors.initials: K
authors.lastname: Jersch
authors.forename: Ina
authors.initials: I
authors.lastname: Hainmann
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Zieger
authors.forename: Daniela
authors.initials: D
authors.lastname: Wunderle
authors.forename: Anja
authors.initials: A
authors.lastname: Busse
authors.forename: Barbara
authors.initials: B
authors.lastname: Zieger
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D018797:Cell Cycle Proteins; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D005091:Exons; D005260:Female; D020558:GTP Phosphohydrolases / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D005796:Genes / Q000235:genetics; D006801:Humans; D007438:Introns; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000378:metabolism; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D058112:Septins; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D018797:Cell Cycle Proteins; D018076:DNA, Complementary; D007527:Isoenzymes; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D020558:GTP Phosphohydrolases; C109366:SEPTIN5 protein, human; C464445:SEPTIN8 protein, human; C491612:Sept5 protein, mouse; D058112:Septins