Analysis of the phase behavior of the aqueous poly(ethylene glycol)-Ficoll system.
The PEG-Ficoll polymer phase system is one that has been overlooked in the past for biotechnology applications because of the stability of its emulsions. However, new applications, such as emulsion coating of cells, are appearing that rely on this very property. Ficoll is highly polydisperse and multimodal with three distinct Ficoll peaks in gel permeation chromatography. As a result, the transition between one-phase and two-phase systems is blurred and the binodials obtained through turbidometric titration and tie-line analysis differ significantly. Moreover, since the three Ficoll peaks partition differently, tie-line analysis cannot be described by a simple model of the aqueous two-phase system. A simple modification to the model allowed for excellent fit, and this modification may prove well-suited for the many practical cases where aqueous two-phase systems fail to display parallel tie-lines as implicitly assumed in the simpler model.
Croll
Tristan
T
Department of Chemical Engineering, University of Queensland, Brisbane, Queensland 4072, Australia.
Munro
Peter D
PD
Winzor
Donald J
DJ
Trau
Matt
M
Nielsen
Lars K
LK
D002845:Chromatography; D003198:Computer Simulation; D004655:Emulsions / Q000737:chemistry*; D005362:Ficoll / Q000737:chemistry*; D046911:Macromolecular Substances; D008956:Models, Chemical; D044367:Phase Transition; D011092:Polyethylene Glycols / Q000737:chemistry*; D015203:Reproducibility of Results; D012492:Salts / Q000737:chemistry*; D012680:Sensitivity and Specificity; D014867:Water / Q000737:chemistry*
D004655:Emulsions; D046911:Macromolecular Substances; D012492:Salts; D014867:Water; D005362:Ficoll; D011092:Polyethylene Glycols