title: [Differential gene expression of liver carcinoma cell after transfection of by ARL-1 with microarray].
abstract: OBJECTIVE
  To study the difference of the gene expression profile and to identify the different expression after transfection of the ARL-1 gene.
  
  
  METHODS
  The cDNA probes were synthesized from total RNA of study group and control group, which was differentially hybridized to cDNA chips and confirmed by a gene specific semiquantitative reverse transcription polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  Six kinds of gene expression were increased and 9 kinds of gene expression were decreased. The findings were correlated with protein metabolism, signal pathway, metastasis, and drug resistance.
  
  
  CONCLUSIONS
  cDNA chips showed that gene expression profile of liver carcinoma cell was changed after transfection of the ARL-1 gene. It is a useful method in understanding the mechanism of drug resistance.
authors.affiliation: Department of Surgery, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Da-nian
authors.initials: DN
authors.lastname: Tang
authors.forename: Yong-guo
authors.initials: YG
authors.lastname: Li
authors.forename: Jun-min
authors.initials: JM
authors.lastname: Wei
authors.forename: Xiang-dong
authors.initials: XD
authors.lastname: Yang
authors.forename: Min-wei
authors.initials: MW
authors.lastname: Zhu
authors.forename: Pei
authors.initials: P
authors.lastname: Wu
authors.forename: Xiong-ying
authors.initials: XY
authors.lastname: Miao
mesh_terms: D000449:Aldehyde Reductase / Q000235:genetics*; D000074408:Aldo-Keto Reductases; D019008:Drug Resistance, Neoplasm; D020869:Gene Expression Profiling; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D014162:Transfection
chemical_list: C120471:AKR1B10 protein, human; D000074408:Aldo-Keto Reductases; D000449:Aldehyde Reductase