[Determination of chlorogenic acid and eriodictyol-7-O-beta-D-glucuronide in Pyrrosia by RP-HPLC].
OBJECTIVE
To develop a method for determination of chlorogenic acid and eriodictyol-7-O-beta-D-glucuronide in Pyrrosia of different species and different places of origin by RP-HPLC.


METHODS
Chromatography was performed using a C18 column with mobile phase of methanol-water-phosphoric acid (50:200:0.2). The monitoring wavelength was 284 nm.


RESULTS
The linear ranges were 0.01-5.0 micrograms (r = 0.9997) and 0.004-5.0 micrograms (r = 0.9997), the recoveries were 97.1% (n = 8, RSD = 2.7%) and 98.8% (n = 9, RSD = 2.5%) for chlorogenic acid and eriodictyol-7-O-beta-D-glucuronide, respectively.


CONCLUSIONS
The method was employed to the analysis of 21 samples of Pyrrosia. The contents of compounds vary greatly depending on the species used, place of collection and time of harvesting. The HPLC method is sensitive, rapid and can be used to control the quality of Pyrrosia and to guide reasonable season of harvesting.
Ma
Chen
C
Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050, China. mach@imm.ac.cn
Zhou
Yuan
Y
Liu
Ai-ru
AR
D002726:Chlorogenic Acid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D044950:Flavanones / Q000032:analysis*; D005965:Glucuronates / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry; D029621:Polypodiaceae / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005965:Glucuronates; C068767:eriodictyol 7-glucuronide; D002726:Chlorogenic Acid