[Preparation and identification of monoclonal antibodies against human apoptosis-related protein TFAR19].
OBJECTIVE
To obtain mouse anti-human TFAR19 monoclonal antibodies for further study of the structure and function of the apoptosis-related protein TFAR19.


METHODS
BALB/c mice were immunized with recombinant human TFAR19, hybridoma cells were screened by cell fusion and subcloning approach. The monoclonal antibodies were identified by ELISA and Western blotting.


RESULTS
Three hybridoma cells (C1, C10, 2C12) stable in secreting anti-TFAR19 monoclonal antibodies were obtained. The monoclonal antibodies showed high specificity and high titer to TFAR19 with various affinity. All of them belong to IgG1 subclass.


CONCLUSIONS
These monoclonal antibodies could bind specifically to TFAR19 protein.
Chen
Y
Y
Department of Immunology, School of Basic Medical Sciences, Peking University, Beijing 100083, China. yingyu_chen@yahoo.com
Zhang
Y
Y
Sun
R
R
Song
Q
Q
Di
C
C
Ma
D
D
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000918:Antibody Specificity; D051017:Apoptosis Regulatory Proteins; D015153:Blotting, Western; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009363:Neoplasm Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D051017:Apoptosis Regulatory Proteins; D009363:Neoplasm Proteins; C117283:PDCD5 protein, human; C486488:Pdcd5 protein, mouse