[Differential expression of the epithelial membrane protein 1 of laryngeal carcinoma].
OBJECTIVE
In order to explore pathogenic mechanism of laryngeal carcinoma, the involved genes were identified in larynx carcinogenesis by comparing the gene expression profile in matched primary normal epithelial cells and primary laryngeal carcinoma cells from the same patients.


METHODS
A cDNA microarray analysis consisting of 11,431 human genes revealed significant changes in the expression of 35 genes, with 8 genes being up-regulated and 27 being down-regulated. The epithelial membrane protein-1 (EMP-1) is one of the down-regulated genes. EMP-1 expression in various kinds of laryngeal carcinoma was determined by semi-quantitative reverse transcriptase polymerase chain reaction (qRT-PCR).


RESULTS
The EMP-1 mRNA levels in all laryngeal carcinoma cells was significantly lower than that in the matched primary normal epithelial cells (P < 0.05) and were not correlated to the stage and differentiation of laryngeal carcinoma (P > 0.05).


CONCLUSIONS
The EMP-1 expression was correlated to larynx carcinogenesis and may be helpful to elucidate the pathogenic mechanism in laryngeal carcinoma.
Liu
Ye-hai
YH
Department of Head and Neck Surgery, Cancer Hospital, CAMS, PUMC, Beijing 100021, China.
Tang
Ping-zhang
PZ
Xu
Zhen-gang
ZG
Qi
Yong-fa
YF
Ding
Fang
F
Zhang
Li-yong
LY
Wang
Hai-tao
HT
Liu
Zhi-hua
ZH
D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins; D020411:Oligonucleotide Array Sequence Analysis; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D009363:Neoplasm Proteins; D011956:Receptors, Cell Surface; C097051:epithelial membrane protein-1