title: Characterisation of a gene cluster in Fugu rubripes containing the complement component C4 gene.
abstract: In this study, we describe the characterisation of the complement component C4 gene in Fugu rubripes. The Fugu C4 gene, orthologous to the tetrapod C4 gene, encompasses a genomic span of 9702 base pairs and contains 41 exons, encoding the typical C4 three-chain polypeptide. The gene encodes a protein containing 1703 amino acids. The Fugu C4 protein demonstrates the presence of 25 conserved cysteine residues, as well as conservation of the functionally important thioester site. Complete sequencing of one cosmid and sequence scans from a cluster of 18 overlapping BAC clones, centering around the C4 gene, have identified the short-range linkage with five orthologous human genes mapping to the Major Histocompatibility Complex (MHC) including: tenascin X (TNX); cytochrome P450, subfamily XXIA, polypeptide 2 (CYP21A2); allograft inflammatory factor 1 (AIF1) and casein kinase 2, beta polypeptide (CSNK2B), all found in the MHC class III region; and retinoid X receptor, beta (RXRB),which resides in the MHC extended class II region. To date, this syntenic association of the Fugu C4 and other MHC class III region genes has not been observed in other teleost fish. Data from the recent whole-genome shotgun assemblies reveal the Fugu MHC-related cluster of genes to be flanked predominantly by genes mapping to human chromosomes 7 and 19. All of the six identified Fugu MHC-related genes have been characterised at the genomic level.
authors.affiliation: Comparative Genomics, MRC UK HGMP Resource Centre, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SB, UK. jsambroo@hgmp.mrc.ac.uk
authors.forename: Jennifer G
authors.initials: JG
authors.lastname: Sambrook
authors.forename: R Duncan
authors.initials: RD
authors.lastname: Campbell
authors.forename: Greg
authors.initials: G
authors.lastname: Elgar
mesh_terms: D000595:Amino Acid Sequence; D003176:Complement C3 / Q000235:genetics; D003181:Complement C4 / Q000235:genetics*; D020451:Contig Mapping; D004247:DNA / Q000737:chemistry / Q000235:genetics; D023061:Gene Order; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013255:Steroid 21-Hydroxylase / Q000235:genetics; D023741:Takifugu / Q000235:genetics*; D019063:Tenascin / Q000235:genetics
chemical_list: D003176:Complement C3; D003181:Complement C4; D019063:Tenascin; C081865:tenascin X; D004247:DNA; D013255:Steroid 21-Hydroxylase