title: [Single nucleotide polymorphisms in CAPN10 gene of Chinese population and its correlation with type 2 diabetes mellitus in Han people of northern China].
abstract: OBJECTIVE
  To investigate the distribution of the single nucleotide polymorphisms (SNPs) in CAPN10 gene in Chinese population and their relation with type 2 diabetes mellitus in Han people of Northern China.
  
  
  METHODS
  CAPN10 gene was sequenced to detect SNPs in 27 samples of different nationalities in China. 5 SNPs were genotyped with single-base extension (SBE) method to perform case-control study in 156 normal Han people of Northern China and 173 type 2 diabetes and the 3 positive loci reported in the article were performed haplotype analysis. One positive locus was also analyzed with transmission-disequilibrium test (TDT) and sib transmission-disequilibrium test (STDT) in 68 type 2 diabetes pedigrees (377 cases).
  
  
  RESULTS
  A total of 40 SNPs were identified in length of 8,936 bp, with an average of 1 in every 223 bp; The SNPs in CAPN10 gene did not distribute evenly and the SNPs in Chinese was different from that reported in American Mexicans. There was no significantly statistical difference in the allele frequency of the 5 SNPs between case and control (P > 0.05), and the haplotype frequencies in the two groups were not much different (P > 0.05). There was no positive results in TDT and STDT analysis (P > 0.05).
  
  
  CONCLUSIONS
  The SNP distribution of CAPN10 gene varies with different nationalities. The studied SNPs in CAPN10 gene may not be the major susceptibility ones of type 2 diabetes mellitus in Han people of Northern China.
authors.affiliation: National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
authors.forename: Hong-xia
authors.initials: HX
authors.lastname: Sun
authors.forename: Kui-xing
authors.initials: KX
authors.lastname: Zhang
authors.forename: Wei-nan
authors.initials: WN
authors.lastname: Du
authors.forename: Jin-xiu
authors.initials: JX
authors.lastname: Shi
authors.forename: Zheng-wen
authors.initials: ZW
authors.lastname: Jiang
authors.forename: Jin
authors.initials: J
authors.lastname: Zuo
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Zhu
authors.initials: Z
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: Shen
authors.forename: Zhi-jian
authors.initials: ZJ
authors.lastname: Yao
authors.forename: Bo-qin
authors.initials: BQ
authors.lastname: Qiang
authors.forename: Jian-mei
authors.initials: JM
authors.lastname: Hang
authors.forename: Heng
authors.initials: H
authors.lastname: Wang
authors.forename: Fu-de
authors.initials: FD
authors.lastname: Fang
mesh_terms: D000483:Alleles; D044466:Asian People; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005006:Ethnicity; D020022:Genetic Predisposition to Disease; D005820:Genetic Testing; D005838:Genotype; D006801:Humans; D020641:Polymorphism, Single Nucleotide