[Identification of protein spots in silver-stained two-dimensional gels based on chemically assisted fragmentation by MALDI-TOF-PSD mass spectrometry].
Guanidination of the epsilon-amino group of lysine-terminated tryptic peptides,accomplished with O-methylisourea hydrogen sulfate, converted lysine into homoarginine residues, improving detection in MALDI-MS. Then tryptic peptides were labeled with sulfonic acid groups at the N-termini using 3-sulfopropionic acid NHS-ester chemistry. The derivatives enhanced post-source decay (PSD) fragmentation signals and produced a spectrum containing only y-ions. This facilitated de novo peptide sequencing, so it is an important contribution to unambiguous protein identification in proteome research. This method was successfully applied in nasopharyngeal carcinoma(NPC) proteome study.
Chen
Ping
P
College of Life Science, Hunan Normal University, Changsha 410081, China.
Nie
Song
S
Xie
Jin-Yun
JY
Liang
Song-Ping
SP
Peng
Chong
C
Li
Gui-Yuan
GY
D000595:Amino Acid Sequence; D015180:Electrophoresis, Gel, Two-Dimensional; D008969:Molecular Sequence Data; D011506:Proteins / Q000032:analysis*; D016622:Silver Staining; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D011506:Proteins