Neural trans-differentiation potential of hepatic oval cells in the neonatal mouse brain.
Although the existence of the hepatic oval cell (HOC), the liver stem cell has been known for almost 70 years, little is known about the potential for this adult stem cell to trans-differentiate into cells of other tissues. While their origin remains enigmatic, HOCs share many similarities with hematopoietic stem cells. Recent studies have revealed that a small percentage of HOCs can arise from a bone marrow-derived stem cell source. Here we report that, like bone marrow stem cells, HOCs can survive transplantation to the neonatal mouse brain and show signs of trans-differentiation by adopting the morphology and antigenic phenotype of both macro- and microglia cells. Trans-differentiated microglia cells were functional, showing active phagocytosis when cotransplanted with latex microbeads in vivo. In addition to glial markers, a small number of transplanted HOCs were immunopositive for neuronal markers, but displayed ambiguous phenotype, making their characterization difficult.
Deng
Jie
J
Department of Pathology, Immunology and Laboratory Medicine, University of Florida, Gainesville, FL 32610, USA.
Steindler
Dennis A
DA
Laywell
Eric D
ED
Petersen
Bryon E
BE
D000818:Animals; D000831:Animals, Newborn; D000943:Antigens, Differentiation / Q000096:biosynthesis; D000950:Antigens, Ly / Q000096:biosynthesis; D001921:Brain / Q000166:cytology*; D002454:Cell Differentiation; D002470:Cell Survival; D002478:Cells, Cultured; D003997:Dicarbethoxydihydrocollidine / Q000494:pharmacology; D006085:Graft Survival; D049452:Green Fluorescent Proteins; D018189:Immunomagnetic Separation; D020547:Lateral Ventricles / Q000166:cytology; D008099:Liver / Q000166:cytology* / Q000187:drug effects; D008164:Luminescent Proteins / Q000096:biosynthesis / Q000235:genetics; D008565:Membrane Proteins / Q000096:biosynthesis; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D017628:Microglia / Q000166:cytology; D009474:Neurons / Q000166:cytology*; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism
D000943:Antigens, Differentiation; D000950:Antigens, Ly; D008164:Luminescent Proteins; C074650:Ly6a protein, mouse; D008565:Membrane Proteins; D049452:Green Fluorescent Proteins; D003997:Dicarbethoxydihydrocollidine