title: SIR1, an upstream component in auxin signaling identified by chemical genetics.
abstract: Auxin is a plant hormone that regulates many aspects of plant growth and development. We used a chemical genetics approach to identify SIR1, a regulator of many auxin-inducible genes. The sir1 mutant was resistant to sirtinol, a small molecule that activates many auxin-inducible genes and promotes auxin-related developmental phenotypes. SIR1 is predicted to encode a protein composed of a ubiquitin-activating enzyme E1-like domain and a Rhodanese-like domain homologous to that of prolyl isomerase. We suggest a molecular context for how the auxin signal is propagated to exert its biological effects.
authors.affiliation: Section of Cell and Developmental Biology, Division of Biological Sciences, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0116, USA. yzhao@biomail.ucsd.edu
authors.forename: Yunde
authors.initials: Y
authors.lastname: Zhao
authors.forename: Xinhua
authors.initials: X
authors.lastname: Dai
authors.forename: Helen E
authors.initials: HE
authors.lastname: Blackwell
authors.forename: Stuart L
authors.initials: SL
authors.lastname: Schreiber
authors.forename: Joanne
authors.initials: J
authors.lastname: Chory
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D001549:Benzamides / Q000378:metabolism / Q000494:pharmacology; D001665:Binding Sites; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D017930:Genes, Reporter; D007210:Indoleacetic Acids / Q000378:metabolism* / Q000494:pharmacology; D008969:Molecular Sequence Data; D009154:Mutation; D009284:Naphthols / Q000378:metabolism / Q000494:pharmacology; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D018515:Plant Leaves / Q000187:drug effects / Q000254:growth & development; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development; D017434:Protein Structure, Tertiary; D015398:Signal Transduction; D037761:Sirtuins / Q000037:antagonists & inhibitors; D014158:Transcription, Genetic
chemical_list: D029681:Arabidopsis Proteins; D001549:Benzamides; D007210:Indoleacetic Acids; D009284:Naphthols; C476859:SIR1 protein, Arabidopsis; C439060:sirtinol; D000255:Adenosine Triphosphate; D037761:Sirtuins