Preparation of insolubilized-DNA film with three-dimensional network and removal of endocrine disruptors.
A water-insolubilized film was prepared by UV irradiation on a dried DNA film. When a UV-irradiated DNA was examined using a circular dichroism spectroscopy, a double stranded structure was observed as well as that of native DNA. The UV irradiated DNA film was also accumulated intercalating reagents. These results suggested that the double stranded structure was involved in the UV irradiated DNA film with a three-dimensional network. The thymine-thymine dimer formation was suggested to be involved in the cross-linking reactions by the polymerization analysis using poly(dA)-poly(dT) and poly(dG)-poly(dC). We also demonstrate the utilization of the UV-irradiated DNA film as a functional material for the accumulation of harmful DNA intercalating pollutants in aqueous solution. These results suggested that the UV-irradiated DNA film was applicable as a functional material for medical, engineering and environmental sciences.
Yamada
M
M
Division of Bioscience, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo, Hokkaido 060-0810, Japan.
Kato
K
K
Nomizu
M
M
Sakairi
N
N
Ohkawa
K
K
Yamamoto
H
H
Nishi
N
N
D000165:Acridine Orange; D000818:Animals; D002942:Circular Dichroism; D003432:Cross-Linking Reagents; D004247:DNA / Q000302:isolation & purification* / Q000528:radiation effects; D004703:Endocrine System / Q000187:drug effects; D007364:Intercalating Agents; D010777:Photochemistry; D011067:Poly dA-dT / Q000302:isolation & purification / Q000528:radiation effects; D011089:Polydeoxyribonucleotides / Q000302:isolation & purification / Q000528:radiation effects; D011740:Pyrimidine Dimers / Q000737:chemistry / Q000528:radiation effects; D012474:Salmon; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet; D014466:Ultraviolet Rays; D014867:Water; D014874:Water Pollutants, Chemical / Q000302:isolation & purification
D003432:Cross-Linking Reagents; D007364:Intercalating Agents; D011089:Polydeoxyribonucleotides; D011740:Pyrimidine Dimers; D014874:Water Pollutants, Chemical; D014867:Water; C016681:poly(dG).poly(dC); D011067:Poly dA-dT; D004247:DNA; D000165:Acridine Orange