title: [The transcriptional regulation study on human delta globin gene with CAAT box C-->T point mutation in its promoter].
abstract: OBJECTIVE
  To study the transcriptional regulation of human delta globin gene with C-->T point mutation at -64 in its promoter.
  
  
  METHODS
  Human delta globin genes including wild CAAT box and mutant CAAT box (-64C-->T) were separately cloned into eukaryotic expression vector pcDNA3.1 (-)/Myc-His A which was cut out the strong promoter CMV, transfected MEL cells, and induced by DMSO to express. The transcriptional regulation of human delta globin gene was analysed using semi-quantitative RT-PCR.
  
  
  RESULTS
  The expression level of human delta globin gene with mutant CAAT box was 2.2-fold as high as that with wild CAAT box.
  
  
  CONCLUSIONS
  The defective CAAT box of human delta globin gene promoter region may be one of the major reasons for its low expression level.
authors.affiliation: National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yao
authors.forename: Song-sen
authors.initials: SS
authors.lastname: Chen
authors.forename: Ke-gong
authors.initials: KG
authors.lastname: Yang
authors.forename: Xu
authors.initials: X
authors.lastname: Di
authors.forename: An-qi
authors.initials: AQ
authors.lastname: Xiong
authors.forename: You-hong
authors.initials: YH
authors.lastname: Zhang
mesh_terms: D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics*; D005914:Globins / Q000235:genetics*; D006801:Humans; D017354:Point Mutation; D011401:Promoter Regions, Genetic / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; D005914:Globins