title: Establishment and characterization of two squamous cell carcinoma cell lines (HYVC and HMVC) derived from vulva.
abstract: Two cell lines, HYVC and HMVC, were established from cultures of the squamous cell carcinoma removed from the vulva of females of 37 and 46 years old, respectively. These cell lines were very similar in their biological characteristics, such as morphology (polygonal), growth pattern (32-43 hr of population doubling time, 50-25% of plating efficiency), heterotransplantability (1 x 10(7) cells produced squamous cell carcinomas in the nude mice), genetics (75-78 of modal chromosomal number), and producing the tumor markers (SCC and TPA). The HPV-DNA was not detected in either the HYVC or HMVC lines by using L1-PCR methods, however, it was detected in the HYVC using E6/E7-PCR.
authors.affiliation: Dept. Ob. & Gy., Toyoko Hosp., Sch. Of Med. St. Marianna Univ.
authors.forename: Kazushige
authors.initials: K
authors.lastname: Kiguchi
authors.forename: Isamu
authors.initials: I
authors.lastname: Ishiwata
authors.forename: Chieko
authors.initials: C
authors.lastname: Ishiwata
authors.forename: Masanori
authors.initials: M
authors.lastname: Iwata
authors.forename: Bunpei
authors.initials: B
authors.lastname: Ishizuka
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yoshikawa
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Tachibana
authors.forename: Hisashi
authors.initials: H
authors.lastname: Hashimoto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ishikawa
mesh_terms: D000328:Adult; D000818:Animals; D002272:Carcinoembryonic Antigen / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology* / Q000821:virology; D002455:Cell Division; D005260:Female; D006801:Humans; D007621:Karyotyping; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D027383:Papillomaviridae / Q000302:isolation & purification; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured; D014846:Vulvar Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology* / Q000821:virology
chemical_list: D002272:Carcinoembryonic Antigen