Thin layer chromatographic resolution of some 2-arylpropionic acid enantiomers using L-(-)-serine, L-(-)-threonine and a mixture of L-(-)-serine and L-(-)-threonine-impregnated silica gel as stationary phases.
Impregnated silica TLC plates with L-(-)-serine and L-(-)-threonine and a mixture of L-(-)-serine and L-(-)-threonine (1:1) as chiral selectors were prepared to use as chiral stationary phases (CSPs) in thin layer chromatography. The resolution of the enantiomers of 2-arylpropionic drugs, including ibuprofen, ibuproxam, ketoprofen, pranoprofen, benoxaprofen, flurbiprofen and tiaprofenic acid was investigated on these CSPs. A mobile phase system of acetonitrile-methanol-water (16:4:0.5, v/v/v) was used. The spots were detected with iodine vapours and the detection limits were found to range between 0.25 and 0.5 micro g/mL for all racemic compounds investigated. The effect of temperature, pH and concentration of the impregnating chiral selectors on resolution has been studied.
Aboul-Enein
Hassan Y
HY
Pharmaceutical Analysis Laboratory, Biological and Medical Research Department (MBC-03-65), King Faisal Specialist Hospital and Research Centre, PO Box 3354, Riyadh 11211, Saudi Arabia. enein@kfshrc.edu.sa
El-Awady
Mahmoud I
MI
Heard
Charles M
CM
D002855:Chromatography, Thin Layer / Q000379:methods; D005782:Gels; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D010666:Phenylpropionates / Q000737:chemistry / Q000302:isolation & purification*; D012694:Serine / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013237:Stereoisomerism; D013329:Structure-Activity Relationship; D013696:Temperature; D013912:Threonine / Q000737:chemistry*
D005782:Gels; D010666:Phenylpropionates; D012997:Solvents; D013912:Threonine; D012694:Serine; D012822:Silicon Dioxide