Qualitative and quantitative determination of yohimbine in authentic yohimbe bark and in commercial aphrodisiacs by HPLC-UV-API/ MS methods.
The development and validation of a rapid qualitative and quantitative method based on an HPLC-UV-MS technique with atmospheric pressure chemical ionisation and electrospray ionisation for the analysis of yohimbine in a number of commercial aphrodisiac products is reported. HPLC with multiple-stage mass spectrometry experiments allowed the identification of the target compound and increased the selectivity of complex analyses such as those involved with multi-botanical preparations. The precision and the robustness of the method were improved by the use of two internal standards: codeine for UV detection and deuterium-labelled yohimbine for MS detection. Twenty commercial aphrodisiac preparations were analysed and the amount of yohimbine measured and expressed as the maximal dose per day suggested on product labels ranged from 1.32 to 23.16 mg.
Zanolari
Boris
B
Université de Lausanne, Institut de Pharmacognosie et Phytochimie, BEP, CH-1015 Lausanne, Switzerland.
Ndjoko
Karine
K
Ioset
Jean-Robert
JR
Marston
Andrew
A
Hostettmann
Kurt
K
D001046:Aphrodisiacs / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry; D015394:Molecular Structure; D032068:Pausinystalia / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000032:analysis; D021281:Quantitative Structure-Activity Relationship; D014466:Ultraviolet Rays; D015016:Yohimbine / Q000032:analysis* / Q000737:chemistry
D001046:Aphrodisiacs; D010936:Plant Extracts; D015016:Yohimbine