title: Acceleration of DNA strand exchange reaction by cationic polymers.
abstract: In previous reports, we have demonstrated that the cationic comb-type copolymers (PLL-g-Dex) having a poly-L-lysine main chain and dextran side chains stabilized DNA duplex and triplex. Furthermore, the copolymer was found to accelerate more than 20,000 folds strand exchange reaction between 20 bp duplex and its homologous single strand (1). This study was designed to inspect structural factor of polycationic moieties in acceleration effects. To this end, polypeptides containing lysine or arginine moieties as cationic groups were examined in their potency to accelerating strand exchange reaction. It was shown that arginine-rich peptide showed higher accelerating effect than lysine peptide when total positive charges of peptides were kept constant.
authors.affiliation: Department of Biomolecular Engineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori, Yokohama 226-8501, Japan.
authors.forename: Kaori
authors.initials: K
authors.lastname: Tajima
authors.forename: Won-Jong
authors.initials: WJ
authors.lastname: Kim
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Akaike
authors.forename: Atsushi
authors.initials: A
authors.lastname: Maruyama
mesh_terms: D000595:Amino Acid Sequence; D002412:Cations; D004247:DNA / Q000737:chemistry*; D004735:Energy Transfer; D005453:Fluorescence; D008969:Molecular Sequence Data; D011108:Polymers / Q000737:chemistry*
chemical_list: D002412:Cations; D011108:Polymers; D004247:DNA