The contributions of the alpha 2 beta 1 integrin to vascular thrombosis in vivo.
The alpha 2 beta 1 integrin serves as a receptor for collagens, laminin, and several other nonmatrix ligands. Many studies have suggested that the alpha 2 beta 1 integrin is a critical mediator of platelet adhesion to collagen within the vessel wall after vascular injury and that the interactions of the platelet alpha 2 beta 1 integrin with subendothelial collagen after vascular injury are required for proper hemostasis. We have used the alpha 2 beta 1 integrin-deficient mouse to evaluate the contributions of the alpha 2 beta 1 integrin in 2 in vivo models of thrombosis. Studies using a model of endothelial injury to the carotid artery reveal that the alpha 2 beta 1 integrin plays a critical role in vascular thrombosis at the blood-vessel wall interface under flow conditions. In contrast, the alpha 2 beta 1 integrin is not required for the formation of thrombi and pulmonary emboli following intravascular injection of collagen. Our results are the first to document a critical in vivo role for the alpha 2 beta 1 integrin in thrombus formation at the vessel wall under conditions of shear following vascular injury.
He
Li
L
Department of Pathology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.
Pappan
Loretta K
LK
Grenache
David G
DG
Li
Zhengzhi
Z
Tollefsen
Douglas M
DM
Santoro
Samuel A
SA
Zutter
Mary M
MM
D000818:Animals; D002339:Carotid Arteries; D003094:Collagen / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000473:pathology; D038982:Integrin alpha2beta1 / Q000235:genetics / Q000502:physiology*; D007700:Kinetics; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D010976:Platelet Count; D011655:Pulmonary Embolism / Q000139:chemically induced / Q000209:etiology; D011961:Receptors, Fc / Q000235:genetics / Q000502:physiology; D013927:Thrombosis / Q000209:etiology*
D038982:Integrin alpha2beta1; D011961:Receptors, Fc; D003094:Collagen