Enzymatic incorporation of an unnatural base pair between 4-propynyl-pyrrole-2-carbaldehyde and 9-methyl-imidazo [(4,5)-b]pyridine into nucleic acids.
A hydrophobic unnatural nucleoside of 4-propynylpyrrole-2-carbaldehyde (designated as Pa') was synthesized to improve its affinity with a pairing partner, 9-methyl-imidazo[(4,5)-b]pyridine (Q), in enzymatic incorporation. In single-nucleotide insertion experiments using the Klenow fragment, the substrate of Pa' (dPa'TP) was efficiently incorporated opposite Q in the template strand, as compared to the incorporation of pyrrole-2-carbaldehyde (dPaTP), which was previously developed.
Mitsui
Tsuneo
T
Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
Kimoto
Michiko
M
Harada
Yoko
Y
Sato
Akira
A
Kitamura
Aya
A
To
Taiko
T
Hirao
Ichiro
I
Yokoyama
Shigeyuki
S
D020029:Base Pairing; D007093:Imidazoles / Q000737:chemistry*; D009696:Nucleic Acids / Q000737:chemistry*; D011725:Pyridines / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*
C476820:4-propynylpyrrole-2-carbaldehyde; C475126:9-methylimidazo((4,5)-b)pyridine; D007093:Imidazoles; D009696:Nucleic Acids; D011725:Pyridines; D011758:Pyrroles