title: [Application of mass spectrometry in the proteome research].
abstract: Electrospray ionization(ESI) and matrix-assisted laser desorption ionization(MALDI) are two kinds of newly-developed mass spectrometry techniques. The application of mass spectrometry is described in the proteome research such as MS charting, determination of molecular weight, peptide mass fingerprinting(PMF) and peptide sequence validation. The advantages of mass spectrometry are sensitive, accurate, rapid, high-throughput and automated. They have been proved a powerful technique and can be used as an important method for proteome research.
authors.affiliation: Department of Microbiology Test, Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Liu
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Zhuang
authors.forename: Pingjian
authors.initials: P
authors.lastname: Deng
authors.forename: Shisong
authors.initials: S
authors.lastname: Fang
authors.forename: Jin
authors.initials: J
authors.lastname: Zhao
mesh_terms: D013058:Mass Spectrometry / Q000379:methods*; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry; D020543:Proteome / Q000737:chemistry*; D012680:Sensitivity and Specificity; D020539:Sequence Analysis, Protein; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D010455:Peptides; D020543:Proteome