title: Comparative analysis of the Bradyrhizobium japonicum sucA region.
abstract: To study the adjustments made to the tricarboxylic acid cycle during symbiosis of nitrogen-fixing rhizobia with their host legumes, we have characterized the genes encoding the alpha-ketoglutarate dehydrogenase enzyme complex in Bradyrhizobium japonicum. The genes were arranged in the order sucA-sucB-scdA-lpdA, where scdArepresents a short-chain dehydrogenase gene (GenBank accession No. AY049030). All four genes appeared to be co-transcribed, an arrangement that is so far unique to B. japonicum. The mdh gene, encoding malate dehydrogenase, was located upstream of the sucA operon, and its primary transcript appeared to be monocistronic. Primer extension indicated that the sucA operon and mdh were transcribed from typical housekeeping promoters.
authors.affiliation: Department of Biochemistry, University of Missouri, Columbia 65211, USA.
authors.forename: Laura S
authors.initials: LS
authors.lastname: Green
authors.forename: James K
authors.initials: JK
authors.lastname: Waters
authors.forename: Shanelle
authors.initials: S
authors.lastname: Ko
authors.forename: David W
authors.initials: DW
authors.lastname: Emerich
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D020369:Bradyrhizobium / Q000201:enzymology / Q000235:genetics*; D002874:Chromosome Mapping; D002952:Citric Acid Cycle; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D007655:Ketoglutarate Dehydrogenase Complex / Q000737:chemistry / Q000235:genetics*; D008291:Malate Dehydrogenase / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009876:Operon; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D016415:Sequence Alignment; D014158:Transcription, Genetic
chemical_list: D010088:Oxidoreductases; D008291:Malate Dehydrogenase; D007655:Ketoglutarate Dehydrogenase Complex