title: New integration vector using a cellulase gene as a screening marker for Lactobacillus.
abstract: The new integration vector for Lactobacillus, pJC4, was developed using the extracellular endoglucanase A gene (celA) of Clostridium thermocellum as a screening marker. pJC4 was transformed into four Lactobacillus species, Lb. johnsonii, Lb. gasseri, Lb. bulgaricus, and Lb. plantarum. In each species, the pJC4 integrants were easily and accurately detected by the appearance of a clear halo on a cellulase screening plate without any false transformants. Polymerase chain reaction and Southern hybridization indicated that all transformants with clear halos contained pJC4 in their chromosomal DNAs. The celA gene could be a useful screening marker for other lactic acid bacteria.
authors.affiliation: School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Suwon 449-701, South Korea.
authors.forename: Soo Jin
authors.initials: SJ
authors.lastname: Jang
authors.forename: Min Seok
authors.initials: MS
authors.lastname: Ham
authors.forename: Jung Min
authors.initials: JM
authors.lastname: Lee
authors.forename: Sung Kyun
authors.initials: SK
authors.lastname: Chung
authors.forename: Hyong Joo
authors.initials: HJ
authors.lastname: Lee
authors.forename: Jeong Hwan
authors.initials: JH
authors.lastname: Kim
authors.forename: Hae Choon
authors.initials: HC
authors.lastname: Chang
authors.forename: Jong-Hoon
authors.initials: JH
authors.lastname: Lee
authors.forename: Dae Kyun
authors.initials: DK
authors.lastname: Chung
mesh_terms: D002480:Cellulase / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods; D005820:Genetic Testing / Q000379:methods*; D007778:Lactobacillus / Q000235:genetics* / Q000302:isolation & purification; D008828:Microbiological Techniques; D014170:Transformation, Genetic
chemical_list: C074775:CelA endoglucanase; D002480:Cellulase