Preparation of DNA catenanes and observation of their topological structures by atomic force microscopy.
DNA catenanes have been prepared by the reaction of T4 DNA ligase with linear DNA in the presence of nicked DNA. Single molecular images of DNA catenanes and large circular DNAs have been clearly observed by AFM using a tapping mode at room temperature and in an ambient atmosphere.
Yamaguchi
H
H
Department of Macromolecular Science, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan.
Kubota
K
K
Harada
A
A
D011088:DNA Ligases; D004270:DNA, Circular / Q000737:chemistry* / Q000302:isolation & purification* / Q000648:ultrastructure; D018625:Microscopy, Atomic Force / Q000379:methods*; D009690:Nucleic Acid Conformation; D010957:Plasmids / Q000737:chemistry
D004270:DNA, Circular; D011088:DNA Ligases