Both recombinant African catfish LH and FSH are able to activate the African catfish FSH receptor.
LH and FSH are heterodimeric glycoprotein hormones, composed of a common alpha-subunit non-covalently associated with a hormone-specific beta-subunit. Repeated efforts to isolate catfish FSH (cfFSH) have not been successful and only catfish LH (cfLH) has been purified from catfish pituitaries. Recently, however, we succeeded in cloning the cDNA encoding the putative cfFSHbeta; the cDNAs for the alpha- and beta-subunit of cfLH have been cloned before. Here we report the expression of biologically active cfLH and cfFSH in the soil amoeba, Dictyostelium discoideum. The biological activity of the recombinant hormones was analyzed using cell lines transiently expressing either the cfLH receptor or the cfFSH receptor. Moreover, a primary testis tIssue culture system served to study the steroidogenic potency of the recombinant hormones. Our results demonstrated that Dictyostelium produced biologically active, recombinant catfish gonadotropins, with recombinant cfLH being almost indistinguishable from its native counterpart, purified from pituitaries. Although recombinant cfFSH has significant effects in the bioassays used in this study, the specific function of native cfFSH in the control of reproduction and its expression patterns are not yet understood.
Vischer
H F
HF
Research Group in Endocrinology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
Granneman
J C M
JC
Linskens
M H K
MH
Schulz
R W
RW
Bogerd
J
J
D000735:Androstenedione / Q000031:analogs & derivatives* / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D002397:Catfishes; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D007986:Luteinizing Hormone / Q000494:pharmacology*; D011962:Receptors, FSH / Q000235:genetics* / Q000502:physiology; D011994:Recombinant Proteins / Q000378:metabolism / Q000494:pharmacology
D017931:DNA Primers; D018076:DNA, Complementary; D011962:Receptors, FSH; D011994:Recombinant Proteins; D000735:Androstenedione; C016496:15-hydroxyandrostenedione; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone