Overexpression of human genes in Drosophila melanogaster by using GAL4 UAS system.
Many human genes determined by genomic sequencing have only few information about their functions. To fill this knowledge gap, the powerful Drosophila genetics was set as a model to elucidate human gene functions effectively. By using germline transformation together with GAL4-UAS system, we studied the possibility of expressing and functionally characterization of human genes in Drosophila. Fifty-four transgenic fly lines corresponding to 10 human genes have been established. When expressed individually by crossing to an array of 6 different GAL4 driver lines, one of these genes, the translation elongation factor 1 alpha 1 (EF1 alpha-1), resulted in abnormal notum and rough eye phenotypes. This study implies the feasibility of systematically screening human gene functions by overexpression in Drosophila.
Ma
Xi-Zhi
XZ
Morgan-Tan International Center for Life Sciences, Institute of Developmental Biology and Molecular Medicine, School of Life Science, Fudan University, Shanghai 200433, China.
Cai
Li-Jun
LJ
Wu
Xiao-Hui
XH
Zhao
Shou-Yuan
SY
Li
Chang-Ben
CB
Deng
Ke-Jing
KJ
D000818:Animals; D030801:Animals, Genetically Modified; D004268:DNA-Binding Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D018014:Gene Transfer Techniques; D005796:Genes / Q000502:physiology*; D006801:Humans; D020648:Peptide Elongation Factor 1 / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
D004268:DNA-Binding Proteins; C052130:GAL4 protein, S cerevisiae; D020648:Peptide Elongation Factor 1; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors