The behavior and effect of isopoly (S-carboxymethyl-L-cysteine) derivatives of nucleic acid bases.
Isopoly(S-carboxymethyl-L-cysteine) derivatives of nucleic acid bases were prepared as antisense compounds. These compounds in vitro have been found to form stable complex with oligo-DNA or RNA. This paper deals with effect of antisense compounds in vivo. The target in this paper is the sequence of the PSD-95 protein linked with NMDA receptor. Excess passing of calcium ions through the loss of the signal pathway without PSD-95 proteins caused by antisense compound. The cells detailing with L-cysteine derivatives showed the lowest percentage of 19.1%. The data were compared with that of phosphotioate antisense compound.
Maruoka
H
H
Materials and Life Science, Graduate School of Osaka University, Yamadaoka 2-1, Suita, Osaka 565-0871, Japan.
Kitaoka
S
S
Tohnai
N
N
Inaki
Y
Y
Hatae
T
T
Tanabe
T
T
D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D016923:Cell Death / Q000187:drug effects; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism
D009419:Nerve Tissue Proteins; D016376:Oligonucleotides, Antisense; D010455:Peptides; D016194:Receptors, N-Methyl-D-Aspartate; C033652:postsynaptic density proteins; C036002:poly(S-carboxymethylcysteine)