Siderophore-mediated strategies of iron acquisition by extraintestinal isolates of Enterobacter spp.
A total of 89 examined Enterobacter isolates belonging to three species: E. cloaceae, E. aerogenes and E. sakazakii, produced iron chelators detected in universal CAS assay. In chemical assays the strains were shown to excrete mostly catecholate (88 strains) and hydroxamate (42 strains) type of siderophores. Forty-one strains produced both catecholate and hydroxamate siderophores whereas one isolate produced only hydroxamate. Besides, the isolates were screened for genes coding for another siderophore: yersiniabactin. The genes for biosynthesis and uptake of yersiniabactin are located on the high-pathogenicity island (HPI) of Yersinia spp. The presence of three marker genes irp1, irp2 and fyuA was estimated by polymerase chain reaction. Two strains: E. aerogenes and E. cloaceae possessed irp1, irp2 and fyuA genes. PCR products of irp1, irp2 and fyuA were of 240, 280 and 780 bp, respectively.
Mokracka
Joanna
J
Department of Microbiology, Institute of Experimental Biology, A. Mickiewicz University, Fredry 10, 61-701 Poznań, Poland.
Kaznowski
Adam
A
Szarata
Małgorzata
M
Kaczmarek
Ewa
E
D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004754:Enterobacter / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D004756:Enterobacteriaceae Infections / Q000382:microbiology; D006801:Humans; D007501:Iron / Q000378:metabolism*; D010636:Phenols; D016133:Polymerase Chain Reaction; D017262:Siderophores / Q000235:genetics / Q000378:metabolism*; D013844:Thiazoles
D004269:DNA, Bacterial; D010636:Phenols; D017262:Siderophores; D013844:Thiazoles; C104398:yersiniabactin; D007501:Iron