Expression of MMP-2, -9 and -13 in cell lines and fresh biopsies of squamous cell carcinomas of the upper aerodigestive tract.
BACKGROUND
The expression patterns of the matrix metalloproteinases (MMP) 2, 9 and 13 have been examined in biopsies and cell lines of squamous cell carcinomas (SCC) of the upper aerodigestive tract to determine the association between MMP expression and the aggressiveness of the tumor.


METHODS
The expression of MMP-2, -9 and -13 was analyzed by immuno-hybridization in 12 keratinocyte cell lines, 13 SCC cell lines and in 63 biopsies from SCCs of the upper aerodigestive tract.


RESULTS
In biopsies a correlation was detected between T-status and the MMP expression [MMP-2 (p < 0.05), MMP-9 (p < 0.01), MMP-13 (p < 0.01)], between N-status and MMP expression [MMP-2 (p < 0.05), MMP-9 (p < 0.01)] and between UICC stage and MMP-9 expression (p < 0.01).


CONCLUSIONS
The MMP-2, MMP-9 and MMP-13 expressions correlate strongly with pathological parameters. The determination of these tumor-biological parameters could help to identify high risk patients with increased frequency of metastatic spread.
Dünne
A A
AA
Department of Otolaryngology, Head and Neck Surgery, Philipps University of Marburg, Deutschhausstrasse 3, 35037 Marburg, Germany. duenne@med.uni-marburg.de
Sesterhenn
A
A
Gerisch
A
A
Teymoortash
A
A
Kuropkat
C
C
Werner
J A
JA
D000328:Adult; D000368:Aged; D001706:Biopsy; D002294:Carcinoma, Squamous Cell / Q000201:enzymology* / Q000473:pathology; D017364:Collagenases / Q000096:biosynthesis; D005260:Female; D006258:Head and Neck Neoplasms / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D008297:Male; D053509:Matrix Metalloproteinase 13; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis; D020782:Matrix Metalloproteinases / Q000096:biosynthesis*; D008875:Middle Aged; D009367:Neoplasm Staging; D014407:Tumor Cells, Cultured
D017364:Collagenases; C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13; D020782:Matrix Metalloproteinases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9