Zinc and mechanical prowess in the jaws of Nereis, a marine worm.
Higher animals typically rely on calcification to harden certain tissues such as bones and teeth. Some notable exceptions can be found in invertebrates: The fangs, teeth, and mandibles of diverse arthropod species have been reported to contain high levels of zinc. Considerable quantities of zinc also occur in the jaws of the marine polychaete worm Nereis sp. High copper levels in the polychaete worm Glycera dibranchiata recently were attributed to a copper-based biomineral reinforcing the jaws. In the present article, we attempt to unravel the role of zinc in Nereis limbata jaws, using a combination of position-resolved state-of-the-art techniques. It is shown that the local hardness and stiffness of the jaws correlate with the local zinc concentration, pointing toward a structural role for zinc. Zinc always is detected in tight correlation with chlorine, suggesting the presence of a zinc-chlorine compound. No crystalline inorganic phase was found, however, and results from x-ray absorption spectroscopy further exclude the presence of simple inorganic zinc-chlorine compounds in amorphous form. The correlation of local histidine levels in the protein matrix and zinc concentration leads us to hypothesize a direct coordination of zinc and chlorine to the protein. A comparison of the role of the transition metals zinc and copper in the jaws of two polychaete worm species Nereis and Glycera, respectively, is presented.
Lichtenegger
Helga C
HC
Department of Chemistry, University of California, Santa Barbara, CA 93106, USA. lichtenegger@pop.tuwien.ac.at
Schöberl
Thomas
T
Ruokolainen
Janne T
JT
Cross
Julie O
JO
Heald
Steve M
SM
Birkedal
Henrik
H
Waite
J Herbert
JH
Stucky
Galen D
GD
D000818:Animals; D002713:Chlorine / Q000032:analysis; D003300:Copper / Q000032:analysis; D006639:Histidine / Q000737:chemistry; D007568:Jaw / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D011077:Polychaeta / Q000033:anatomy & histology*; D013053:Spectrophotometry; D014965:X-Rays; D015032:Zinc / Q000032:analysis* / Q000737:chemistry
D006639:Histidine; D002713:Chlorine; D003300:Copper; D015032:Zinc