title: [Expression of human fibroleukin gene acute on chronic hepatitis B and its clinical significance].
abstract: OBJECTIVE
  To investigate the mRNA and protein expressions of human fibroleukin gene (hfg12) in acute on chronic (AOC) hepatitis B and its clinical significance.
  
  
  METHODS
  Liver tissues were obtained from 23 patients with AOC hepatitis B, 13 patients with chronic hepatitis, and 14 patients with cirrhosis to be examined histologically. Immunohistochemistry and in situ hybridization were used to detect the mRNA and protein expressions of hfg12 in the liver tissues. Double staining was used to the hfg12 positive samples to examine both the hfg12 and fibrin. Four specimens of liver tissue from normal donors were used as controls.
  
  
  RESULTS
  Immunohistochemistry showed that hfg12 was expressed in the liver tissues of 21 out of the 23 patients with AOC hepatitis B (91.30%) and only one out of the 13 patients with chronic hepatitis (7.69%). In situ hybridization showed that hfg12 was expressed in the liver tissues of 13 out of he 23 patients with AOC hepatitis B and in none of the 27 patients with chronic hepatitis or cirrhosis. In patients with AOC hepatitis Kupffer's cell, CD68 positive, was numerous and big, mainly distributed in the necrosis areas. It was identified as the same of hfg12-expressing cells.
  
  
  CONCLUSIONS
  High expression of hfg12 is one of the molecular mechanisms of necrosis of liver cells in AOC hepatitis.
authors.affiliation: Division of Clinical Immunology and Department of Infectious Diseases, Tongji Hospital, Research Institute of Immunology, Tongji Medical College of Huanzhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Chen
authors.forename: Qin
authors.initials: Q
authors.lastname: Ning
authors.forename: Bao-ju
authors.initials: BJ
authors.lastname: Wang
authors.forename: Dong-shen
authors.initials: DS
authors.lastname: Zhang
authors.forename: Fu-ming
authors.initials: FM
authors.lastname: Yan
authors.forename: Yi
authors.initials: Y
authors.lastname: Sun
authors.forename: Dong
authors.initials: D
authors.lastname: Xi
authors.forename: Wei-ming
authors.initials: WM
authors.lastname: Yan
authors.forename: Lian-jie
authors.initials: LJ
authors.lastname: Hao
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D005260:Female; D005340:Fibrinogen / Q000032:analysis / Q000235:genetics*; D019694:Hepatitis B, Chronic / Q000378:metabolism* / Q000469:parasitology; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008297:Male; D009336:Necrosis
chemical_list: C095202:FGL2 protein, human; D005340:Fibrinogen