Expression and promoter analysis of mouse mastrin gene.
Human astrin is a newly identified microtubule-associated protein, which is highly expressed in the testis. Silencing of astrin has resulted in growth arrest and apoptotic cell death. In this study, we describe the cloning and genomic structure of mastrin, the mouse counterpart to astrin. The overall mouse mastrin amino-acid sequence is 66% identical to human astrin. Mastrin protein was demonstrated to localize to mitotic spindles during mitosis. Genomic clones containing mastrin gene were isolated; the gene was found to have 24 exons spanning 24kb of genomic DNA. Deletion analysis of 5(')-flanking sequences demonstrated that the first 120bp proximal to the TATA-less promoter region is necessary for minimal transcription of the mouse mastrin gene.
Chang
Mau-Sun
MS
Department of Medical Research, Mackay Memorial Hospital, 45 Mingshen Road, Tamshui, 251, Taipei, Taiwan.
Chen
Chiung-Ya
CY
Huang
Chang-Jen
CJ
Fan
Chi-Chen
CC
Chu
Jien-Ming
JM
Yang
Yuh-Cheng
YC
D016475:3T3 Cells; D024506:5' Flanking Region; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000737:chemistry; D003001:Cloning, Molecular; D006801:Humans; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D016415:Sequence Alignment; D014018:Tissue Distribution; D014158:Transcription, Genetic; D015533:Transcriptional Activation
D018797:Cell Cycle Proteins; D008869:Microtubule-Associated Proteins; C486663:SPAG5 protein, human; C435983:Spag5 protein, mouse