title: Antioxidant properties of extracts from Alchornea laxiflora (Benth) Pax and Hoffman.
abstract: The antioxidant activities of the leaf and root extracts of Alchornea laxiflora, a plant used locally for the preservation of food items in Nigeria, were evaluated using the ferric thiocyanate method, horseradish peroxidase catalysed oxidation of 2,2 azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), beta-carotene linoleate model system and Fe(2+)/ascorbate/H(2)O(2)-induced rat liver microsomal lipid peroxidation. The crude hexane root (HR), methanol root (MR), methanol leaf (ML) and hexane leaf (HL) extracts from A. laxiflora were tested for antioxidant activities. Antioxidant activity decreased in the following order: HR (76.4%), MR (63%), ML (40%) and HL (38%) at a concentration of 0.05% v/v. The antioxidant activity of HR compared to that of butylated hydroxyanisole (BHA) (80%), a standard antioxidant. The total antioxidant activity (TAA) of the crude extracts suggests that activity is highest in the HR compared with the others. The TAA value was estimated to be 8.0 measured as mm of vitamin C equivalent. Six column chromatographic fractions (FI-FVI) from HR showed antioxidant activity to varying extents in the beta-carotene model system in the order of FII > FI > FVI > FIII > FIV > FV. FII exhibited the highest antioxidant activity in all model systems utilized, it recorded a higher antioxidant activity than BHA and quercetin in the beta-carotene linoleate and Fe(2+)/ascorbate/H(2)O(2). TLC analysis of fraction II revealed the presence of terpenoid compounds (radiant green coloration with 2,4 dinitrophenylhydrazine). Our results suggest that A. laxiflora contains potent natural antioxidants and may therefore be relevant in the preservation of lipid food products, which are prone to oxidation and rancidity.
authors.affiliation: Drug Metabolism and Toxicology Research Laboratories, Department of Biochemistry, College of Medicine University of Ibadan, Ibadan, Nigeria. ofarombi@skannet.com
authors.forename: E Olatunde
authors.initials: EO
authors.lastname: Farombi
authors.forename: Oluyemisi O
authors.initials: OO
authors.lastname: Ogundipe
authors.forename: E
authors.initials: E
authors.lastname: Samuel Uhunwangho
authors.forename: Mary A
authors.initials: MA
authors.lastname: Adeyanju
authors.forename: J
authors.initials: J
authors.lastname: Olarenwaju Moody
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D001205:Ascorbic Acid; D052160:Benzothiazoles; D019665:Euphorbiaceae; D005520:Food Preservatives / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D007501:Iron; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008861:Microsomes / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D018515:Plant Leaves; D018517:Plant Roots; D051381:Rats; D017208:Rats, Wistar; D013451:Sulfonic Acids; D013861:Thiocyanates; D019207:beta Carotene
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D005520:Food Preservatives; D010936:Plant Extracts; D013451:Sulfonic Acids; D013861:Thiocyanates; D019207:beta Carotene; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C103724:ferric thiocyanate; D007501:Iron; D001205:Ascorbic Acid