Automated in vitro selection to obtain functional oligonucleotides.
In vitro selection or systematic evolution of ligand by exponential enrichment (SELEX) has been devised for the identification of high-affinity oligonucleotide aptamers to target molecules. However, the selection process is repetitive and time-consuming. We have developed an automatic for in vitro selection by assembling an affinity chromato-column, a PCR thermal cycler, a HPLC and a sample operation system. Several molecular biology methods were optimized for the machine. Automated selection was used to generate nucleic acid aptamers interacting specifically with an environmental contaminant.
Zhang
H
H
Department of Biological Science and Technology, Faculty of Engineering, University of Tokushima, Tokushima 770-8506, Japan.
Hamasaki
A
A
Toshiro
E
E
Aoyama
Y
Y
Ito
Y
Y
D001331:Automation; D001483:Base Sequence; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D004277:DNA, Single-Stranded / Q000235:genetics / Q000302:isolation & purification; D005821:Genetic Techniques; D009838:Oligodeoxyribonucleotides / Q000235:genetics* / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012641:Selection, Genetic
D004277:DNA, Single-Stranded; D009838:Oligodeoxyribonucleotides