Mechanistic studies of RNA modifying enzymes. RNA pseudouridine synthase and m5cytosine methyl transferase.
RNA has numerous post-transcriptional modifications, but relatively little is known about the enzymes that catalyze such modifications or about the functions of the modified residues. Our laboratory has been engaged in studies of the structure and function of enzymes that catalyze the conversion of Urd residues in RNA to pseudouridine (psi Synthase), and Cyd to 5-methylcytidine methyl transferase (RNA m5C Mtase). The presentation will summarize recent results from our laboratory.
Santi
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Department of Pharmaceutical Chemistry and Biochemistry, University of California, San Francisco, CA 94143, USA.
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D020134:Catalytic Domain; D003545:Cysteine / Q000737:chemistry; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D016680:Genome, Bacterial; D006836:Hydro-Lyases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020543:Proteome; D012313:RNA / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D012329:RNA, Bacterial / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D012359:tRNA Methyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D020543:Proteome; D012329:RNA, Bacterial; D029701:Saccharomyces cerevisiae Proteins; D012313:RNA; D012359:tRNA Methyltransferases; C024845:NCL1 protein, S cerevisiae; D004248:DNA (Cytosine-5-)-Methyltransferases; D006836:Hydro-Lyases; C022479:pseudouridylate synthetase; D003545:Cysteine