Effects of neutral, cationic, and anionic chromium ascorbate complexes on isolated human mitochondrial and genomic DNA.
The relative activities of neutral, cationic, and anionic chromium ascorbate complexes toward isolated human mitochondrial and genomic DNA were investigated at physiologically relevant conditions by agarose gel electrophoresis. A direct relationship between the charge of the Cr(III) species and their DNA-damaging properties was found. The cationic species were found to be fully capable of DNA-cleavage, even in short incubation periods. Incubations were also performed in the presence of amino acids. No apparent effect was observed under the applied experimental conditions to facilitate or prevent damage through the ternary amino acid-Cr-DNA adduct formation or binary chromium-amino acid complex formation.
Ay
Ahmet Nedim
AN
Department of Chemistry, Hacettepe University, Beytepe Campus 06532, Ankara, Turkey.
Zümreoglu-Karan
Birgül
B
Oner
Reyhan
R
Unaleroglu
Canan
C
Oner
Cihan
C
D000596:Amino Acids / Q000378:metabolism; D000838:Anions; D001205:Ascorbic Acid / Q000378:metabolism* / Q000494:pharmacology; D002412:Cations; D002857:Chromium / Q000378:metabolism* / Q000494:pharmacology; D017608:Chromium Compounds / Q000378:metabolism* / Q000494:pharmacology; D003432:Cross-Linking Reagents; D004247:DNA / Q000187:drug effects / Q000378:metabolism*; D018736:DNA Adducts / Q000378:metabolism; D004249:DNA Damage; D004272:DNA, Mitochondrial / Q000187:drug effects* / Q000378:metabolism; D003853:Deoxyribonucleosides / Q000378:metabolism; D003854:Deoxyribonucleotides / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics
D000596:Amino Acids; D000838:Anions; D002412:Cations; D017608:Chromium Compounds; D003432:Cross-Linking Reagents; D018736:DNA Adducts; D004272:DNA, Mitochondrial; D003853:Deoxyribonucleosides; D003854:Deoxyribonucleotides; D002857:Chromium; D004247:DNA; D001205:Ascorbic Acid