title: Alternative splicing of transcripts from crtI and crtYB genes of Xanthophyllomyces dendrorhous.
abstract: Xanthophyllomyces dendrorhous is one of the relevant sources of the carotenoid astaxanthin. In this paper, we describe for the first time cloning of unexpected cDNAs obtained from the crtI and crtYB genes of X. dendrorhous strain UCD 67-385. The cDNA of the crtI gene conserves 80 bp of the first intron, while the cDNA of the crtYB gene conserves 55 bp of the first intron and lacks 111 bp of the second exon. The crtI and crtYB RNAs could be spliced in alternative splice sites, which produced alternative transcripts which could not be translated to active CRTI and CRTYB proteins since they had numerous stop codons in their sequences. The ratio of mature mRNA to alternative mRNA for the crtI gene decreased as a function of the age of the culture, while the cellular content of carotenoids increased. It is possible that splicing to mature or alternative transcripts could regulate the cellular concentrations of phytoene desaturase and phytoene synthase-lycopene cyclase proteins, depending on the physiological or environmental conditions.
authors.affiliation: Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Santiago, Chile.
authors.forename: P
authors.initials: P
authors.lastname: Lodato
authors.forename: J
authors.initials: J
authors.lastname: Alcaino
authors.forename: S
authors.initials: S
authors.lastname: Barahona
authors.forename: P
authors.initials: P
authors.lastname: Retamales
authors.forename: V
authors.initials: V
authors.lastname: Cifuentes
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000235:genetics*; D017398:Alternative Splicing; D001483:Base Sequence; D001487:Basidiomycota / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D051232:Geranylgeranyl-Diphosphate Geranylgeranyltransferase; D019753:Intramolecular Lyases / Q000235:genetics*; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics*
chemical_list: D005656:Fungal Proteins; D010088:Oxidoreductases; C022398:phytoene dehydrogenase; D019883:Alkyl and Aryl Transferases; D051232:Geranylgeranyl-Diphosphate Geranylgeranyltransferase; D019753:Intramolecular Lyases; C070925:lycopene cyclase-isomerase