[Effect of 5,7-dihydroxytryptamine on the distribution pattern of calcitonin gene-related peptide in different motoneuron pools].
OBJECTIVE
The effect of 5,7-dihydroxytryptamine (5,7-DHT) on distribution pattern of calcitonin gene-related peptide (CGRP) in different motoneuron pools was studied in rats.


METHODS
Cholera toxin B subunit coupled with colloidal gold (CB-Au) retrograde labeling combined with CGRP immunocytochemistry technique was mainly used.


RESULTS
In control group, there was a different distribution pattern of CGRP-LI content in soleus motoneuron (SOL-Mn) and extensor digitorum longus motoneuron (EDL-Mn) pools. SOL-Mn pools had a higher ratio of neurons lacking CGRP-LI/weak CGRP-LI and a lower proportion of strongly CGRP-LI labeled ones. Comparing with control, there was a marked shift to increasing of CGRP-LI content in SOL-Mn pool and slight decrease in EDL-Mn pools 14 days after 5,7-DHT. All the SOL-Mns were CGRP-LI positive and the ratio of CGRP-LI strongly positive Mns significantly increased (P < 0.001) while 5,7-DHT did not increase the ratio of CGRP-LI strongly positive Mn in EDL-Mn pool.


CONCLUSIONS
Except muscular motor activity, the different distribution pattern of CGRP-LI content in SOL-Mn and EDL-Mn pools is related to different 5-HT inputs. SOL-Mns may receive more 5-HT inputs.
Shi
G
G
Department of Anatomy, Hebei Medical University, Shijiazhuang 050017, China.
Tan
H
H
Liu
Y
Y
Liu
S
S
Guo
A
A
Wan
X
X
D015116:5,7-Dihydroxytryptamine / Q000494:pharmacology*; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism*; D008297:Male; D009046:Motor Neurons / Q000187:drug effects* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000294:innervation*; D051381:Rats; D017208:Rats, Wistar
D015116:5,7-Dihydroxytryptamine; D015740:Calcitonin Gene-Related Peptide