title: A mechanism of cyclin D1 action encoded in the patterns of gene expression in human cancer.
abstract: Here we describe how patterns of gene expression in human tumors have been deconvoluted to reveal a mechanism of action for the cyclin D1 oncogene. Computational analysis of the expression patterns of thousands of genes across hundreds of tumor specimens suggested that a transcription factor, C/EBPbeta/Nf-Il6, participates in the consequences of cyclin D1 overexpression. Functional analyses confirmed the involvement of C/EBPbeta in the regulation of genes affected by cyclin D1 and established this protein as an indispensable effector of a potentially important facet of cyclin D1 biology. This work demonstrates that tumor gene expression databases can be used to study the function of a human oncogene in situ.
authors.affiliation: Departments of Medical Oncology and Medicine, Dana-Farber Cancer Institute and Harvard Medical School, 44 Binney Street, Boston, MA 02115, USA.
authors.forename: Justin
authors.initials: J
authors.lastname: Lamb
authors.forename: Sridhar
authors.initials: S
authors.lastname: Ramaswamy
authors.forename: Heide L
authors.initials: HL
authors.lastname: Ford
authors.forename: Bernardo
authors.initials: B
authors.lastname: Contreras
authors.forename: Robert V
authors.initials: RV
authors.lastname: Martinez
authors.forename: Frances S
authors.initials: FS
authors.lastname: Kittrell
authors.forename: Cynthia A
authors.initials: CA
authors.lastname: Zahnow
authors.forename: Nick
authors.initials: N
authors.lastname: Patterson
authors.forename: Todd R
authors.initials: TR
authors.lastname: Golub
authors.forename: Mark E
authors.initials: ME
authors.lastname: Ewen
mesh_terms: D022782:CCAAT-Enhancer-Binding Protein-beta / Q000235:genetics / Q000378:metabolism*; D019938:Cyclin D1 / Q000235:genetics* / Q000378:metabolism*; D030541:Databases, Genetic; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D009369:Neoplasms / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D011401:Promoter Regions, Genetic; D018709:Statistics, Nonparametric; D014407:Tumor Cells, Cultured
chemical_list: D022782:CCAAT-Enhancer-Binding Protein-beta; D009363:Neoplasm Proteins; D019938:Cyclin D1