title: [Determination of ascorbic acid by suppressed chemiluminescence].
abstract: This paper describes a method for determining ascorbic acid by the suppression of DTMC-H2O2 chemiluminescence. Various factors affecting the chemiluminescence determination of ascorbic acid were examined. Under the optimal conditions (1.2 x 10(-4) mol.L-1 of DTMC, 5.0 x 10(-5) mol.L-1 of H2O2, and pH 11.4 Na2HPO4-NaOH), the quenching of the chemiluminescent intensity was proportional to the concentration of ascorbic acid in the range of 1.0 x 10(-7)-8.0 x 10(-6) mol.L-1, and the detection limit was 8.0 x 10(-8) mol.L-1 (S/N = 3). The RSD was 4.6% for 1.0 x 10(-6) mol.L-1 of ascorbic acid (n = 10). The selectivity of this method was high and no making agent required. The proposed method has been applied to the determination of ascorbic acid in real samples. The obtained results were in agreement with those by CuSO4-KI titration.
authors.affiliation: Center for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China.
authors.forename: Quan-li
authors.initials: QL
authors.lastname: Ma
authors.forename: Yong-ping
authors.initials: YP
authors.lastname: Jin
authors.forename: Hong-tao
authors.initials: HT
authors.lastname: You
authors.forename: Hui-min
authors.initials: HM
authors.lastname: Ma
mesh_terms: D001205:Ascorbic Acid / Q000032:analysis*; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D013050:Spectrometry, Fluorescence
chemical_list: D007202:Indicators and Reagents; D001205:Ascorbic Acid