Separation of biogenic amines by micellar electrokinetic chromatography with on-line chemiluminescence detection.
A method of on-line chemiluminescence detection with capillary electrophoresis for biogenic amines (diaminopropane, putrescine, cadaverine and diaminohexane) labeled with N-(4-aminobutyl)-N-ethylisoluminol is reported for the first time. Two separation modes, capillary zone electrophoresis and micellar electrokinetic chromatography (MEKC), were studied. The results show that excellent resolution was achieved in MEKC. Parameters affecting separation process and chemiluminescence detection have been examined in detail. Under the optimum conditions, the baseline separation of four amines was obtained within 7.5 min. The detection limits (S/N=3) of diaminopropane, putrescine, cadaverine and diaminohexane are 3.5 x 10(-8), 3.5 x 10(-8), 3.9 x 10(-8) and 1.2 x 10(-7) M, respectively. The method was applied to the analysis of biogenic amines in lake water.
Liu
Yan-Ming
YM
Department of Chemistry, Wuhan University, Wuhan 430072, China.
Cheng
Jie-Ke
JK
D001679:Biogenic Amines / Q000302:isolation & purification*; D002103:Cadaverine / Q000302:isolation & purification; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003959:Diamines / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D011700:Putrescine / Q000302:isolation & purification; D012680:Sensitivity and Specificity
D001679:Biogenic Amines; D003959:Diamines; D007202:Indicators and Reagents; C009475:trimethylenediamine; D002103:Cadaverine; D011700:Putrescine; C007297:1,6-diaminohexane