title: Establishment and characterization of new B-cell precursor leukemia cell line NALM-35.
abstract: A novel B-cell precursor (BCP) acute lymphoblastic leukemia (ALL) cell line, NALM-35, was established from the peripheral blood of a 40-year-old woman at diagnosis of ALL. Imunophenotyping showed BCP type III characteristics including expression of TdT, CD10, CD19, CD22, CD79a and HLA class II. T-cell and myeloid-associated antigens tested were negative except CD5 and CD28. The surrogate light chains CD179a and CD179b were positive. NALM-35 cells have the morphological appearance of lymphoblasts. Cytogenetic analysis of NALM-35 revealed an abnormal karyotype with 46, XX, add(9)(p11). Southern blot analysis of the immunoglobulin genes status of NALM-35 at 10 months after establishment showed germ line configuration of the kappa and lambda light chain genes, and rearrangement of the mu heavy chain gene. DNA fingerprinting, chromosomal analysis and immunophenotyping proved that NALM-35 was clonally derived from the primary leukemia cells. The established cell line may provide a useful model system and unprecedented opportunities for analyzing the multitude of biological aspects of normal and neoplastic B-lymphocytes and their precursors.
authors.affiliation: Fujisaki Cell Center, Hayashibara Biochemical Labs, Inc., Okayama, Japan. yomatsuo@hayashibara.co.jp
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Matsuo
authors.forename: Hans G
authors.initials: HG
authors.lastname: Drexler
authors.forename: Akira
authors.initials: A
authors.lastname: Harashima
authors.forename: Nobuharu
authors.initials: N
authors.lastname: Fujii
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Ishimaru
authors.forename: Kunzo
authors.initials: K
authors.lastname: Orita
mesh_terms: D000328:Adult; D015703:Antigens, CD; D016172:DNA Fingerprinting; D005260:Female; D015321:Gene Rearrangement; D000949:Histocompatibility Antigens Class II; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics; D016130:Immunophenotyping; D007621:Karyotyping; D015448:Leukemia, B-Cell / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D015703:Antigens, CD; D000949:Histocompatibility Antigens Class II; D007136:Immunoglobulins