title: BldD from Streptomyces coelicolor is a non-essential global regulator that binds its own promoter as a dimer.
abstract: We have shown that the bldD gene of Streptomyces coelicolor, while required for antibiotic production and morphological differentiation, is not essential for viability. We have also demonstrated that BldD forms a higher order complex both in solution and when bound to target DNA. Purified BldD exists in three forms in solution, as a tetramer, dimer and monomer, but only in the dimeric form when bound to its own promoter/operator.
authors.affiliation: Department of Biological Sciences, CW405 Biological Sciences Building, University of Alberta, Edmonton, AB, Canada T6G 2E9.
authors.forename: Marie A
authors.initials: MA
authors.lastname: Elliot
authors.forename: Troy R
authors.initials: TR
authors.lastname: Locke
authors.forename: Claire M
authors.initials: CM
authors.lastname: Galibois
authors.forename: Brenda K
authors.initials: BK
authors.lastname: Leskiw
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D019281:Dimerization; D017353:Gene Deletion; D005798:Genes, Bacterial; D010641:Phenotype; D017354:Point Mutation; D011401:Promoter Regions, Genetic; D020836:Protein Structure, Quaternary; D012996:Solutions; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; C111306:BldD protein, Streptomyces coelicolor; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D012996:Solutions; D014157:Transcription Factors