title: Quantitation of talinolol and other beta-blockers by capillary electrophoresis for in vitro drug absorption studies.
abstract: A capillary zone electrophoresis method is described for the enantioseparation of talinolol using heptakis(2,3-diacetyl-6-sulfo)-beta-cyclodextrin (HDAS-beta-CD) as a chiral selector. After liquid-liquid extraction of talinolol from physiological solution, electrokinetic injection was employed to improve the sensitivity. The use of a coated capillary was necessary to achieve stable and reproducible enantioseparations. A baseline separation of the talinolol enantiomers was achieved in less than 10 min using 100 mM phosphate solution as background electrolyte and pH 3.5, at the presence of 3.0 mM HDAS-beta-CD and at 20 degrees C. In addition, this analytical condition proved to be useful for the enantioseparation of a number of other beta-blocking agents such as alprenolol, atenolol, bisoprolol, celiprolol, metipranolol, oxprenolol, and sotalol. For determining talinolol, the method could be validated in terms of precision, accuracy and linearity, and was found to be suitable in determination of talinolol enantiomers in highly diluted samples obtained from in vitro experiments.
authors.affiliation: Department of Pharmaceutical Technology, Eberhard-Karls-University of Tübingen, Auf der Morgenstelle 8, D-72076 Tübingen, Germany.
authors.forename: Bilal
authors.initials: B
authors.lastname: Awadallah
authors.forename: Peter C
authors.initials: PC
authors.lastname: Schmidt
authors.forename: Ulrike
authors.initials: U
authors.lastname: Holzgrabe
authors.forename: Martin A
authors.initials: MA
authors.lastname: Wahl
mesh_terms: D000042:Absorption; D000319:Adrenergic beta-Antagonists / Q000032:analysis* / Q000493:pharmacokinetics; D018938:Caco-2 Cells; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000592:standards; D006801:Humans; D007700:Kinetics; D011412:Propanolamines / Q000032:analysis* / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D013237:Stereoisomerism
chemical_list: D000319:Adrenergic beta-Antagonists; D011412:Propanolamines; C011550:talinolol