A novel chitosanase from Bacillus sp. strain K17: gene cloning and expression in Escherichia coli.
The gene encoding a novel chitosanase from Bacillus sp. strain K17 was cloned and sequenced. The nucleotide sequence of the gene contained an open reading frame corresponded to a protein of 453 amino acids. The deduced amino acid sequence of the K17 chitosanase exhibited the highest homology to those of family 8 glycanases, suggesting that the enzyme belonged to family 8.
Yatsunami
Rie
R
Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori-ku, Yokohama 226-8501, Japan.
Sakihama
Yuri
Y
Suzuki
Mamie
M
Fukazawa
Tetsuya
T
Shimizu
Shinji
S
Sunami
Tomoko
T
Endo
Kimiko
K
Takénaka
Akio
A
Nakamura
Satoshi
S
D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology*; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D004926:Escherichia coli / Q000201:enzymology; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D017931:DNA Primers; D011994:Recombinant Proteins; D006026:Glycoside Hydrolases; C019868:chitosanase