An enzyme electrode based on lipoxygenase immobilized in gelatin for selective determination of essential fatty acids.
An enzyme electrode for the specific determination of omega-3 and omega-6 fatty acids from the mixture of essential fatty acids (EFAs) was developed by using lipoxygenase (LOX) (EC 1.13.11.12) from soy beans in combination with a dissolved oxygen (DO) probe. The enzyme electrode showed different sensitivities for linoleic (LA) and alpha-linolenic acids (ALA), the most common essential fatty acids. Enzyme electrode response depends linearly on LA concentration between 12.8-160.5 microM and ALA concentration between 3.8-18.9 microM in borate buffer, 0.2 M at pH 9.0. However, in phosphate buffer 0.2 M at pH 6.0 linearity is in the range of 7.5-22.5 microM of ALA concentration at 5 minutes response times. Moreover, maximum electrode response was found in borate buffer at pH 9.0 and 30 degrees C.
Timur
Suna
S
Department of Biochemistry, Faculty of Science, Ege University, Bornova-Izmir, Turkey. timur@sci.ege.edu.tr
Onal
Seçil
S
Akyilmaz
Erol
E
Telefoncu
Azmi
A
D015374:Biosensing Techniques / Q000379:methods* / Q000592:standards; D002138:Calibration; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005502:Food; D005780:Gelatin; D006863:Hydrogen-Ion Concentration; D019787:Linoleic Acid / Q000032:analysis* / Q000378:metabolism; D008084:Lipoxygenase / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013025:Glycine max / Q000201:enzymology; D013696:Temperature
D004800:Enzymes, Immobilized; D005780:Gelatin; D019787:Linoleic Acid; D008084:Lipoxygenase