title: [Effects of asbestos on P53 protein expression in V79 cells].
abstract: OBJECTIVE
  To explore the effects of physical and chemical properties of asbestos on mutagenesis/carcinogenesis.
  
  
  METHODS
  The P53 protein expression in V79 cells exposed to asbestos suspension and filtrate was studied using immunohistochemical method.
  
  
  RESULTS
  After asbestos suspension and filtrate treatment of V79 cells, the value of hue for indicating P53 protein expression was significantly lowered, and such abnormal expression was especially observed in binucleate cells and polykaryocytes.
  
  
  CONCLUSIONS
  The above data demonstrated that abnormal accumulation of P53 protein in V79 cells was related to asbestos exposure and that asbestos suspension and filtrate had even greater effect on the cells at the segmentation period.
authors.affiliation: Department of Hygiene Toxicology, West China School of Public Health, Sichuan University, Chengdu 610041, China.
authors.forename: Lingyi
authors.initials: L
authors.lastname: Liu
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Hu
authors.forename: Dingguo
authors.initials: D
authors.lastname: Pang
authors.forename: Wenzhen
authors.initials: W
authors.lastname: Peng
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D001194:Asbestos / Q000633:toxicity*; D002273:Carcinogens / Q000633:toxicity*; D002478:Cells, Cultured; D006224:Cricetinae; D005347:Fibroblasts / Q000166:cytology; D007150:Immunohistochemistry; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D002273:Carcinogens; D016159:Tumor Suppressor Protein p53; D001194:Asbestos