Molecular cloning, expression, self-assembly, antigenicity, and seroepidemiology of a genogroup II norovirus isolated in France.
Virus-like particles of Dijon171/96 virus, a genogroup II norovirus, were expressed in a baculovirus system and were used for a seroepidemiological study of 1078 age-stratified human sera collected in Dijon, France. The results showed a seroprevalence of 74.1%. Furthermore, we showed that murine antibodies generated against recombinant Dijon171/96 virus, and human antibodies recognized discontinuous epitopes on the particles.
Nicollier-Jamot
Béatrice
B
Microbiologie Médicale et Moléculaire, Facultés de Médecine et Pharmacie, Université de Bourgogne, 21033 Dijon cedex, France.
Pico
Valérie
V
Pothier
Pierre
P
Kohli
Evelyne
E
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002139:Caliciviridae / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification*; D017250:Caliciviridae Infections / Q000453:epidemiology* / Q000276:immunology; D036022:Capsid Proteins / Q000235:genetics / Q000302:isolation & purification; D002648:Child; D002675:Child, Preschool; D003001:Cloning, Molecular / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005602:France / Q000453:epidemiology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007223:Infant; D051379:Mice; D008875:Middle Aged; D008969:Molecular Sequence Data; D016036:Seroepidemiologic Studies; D012703:Serotyping / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000914:Antibodies, Viral; D036022:Capsid Proteins; D007074:Immunoglobulin G