title: Solid phase synthesis of phosphorothioate oligonucleotides utilizing diethyldithiocarbonate disulfide (DDD) as an efficient sulfur transfer reagent.
abstract: Diethyldithiodicarbonate (DDD), a cheap and easily prepared compound, is found to be a rapid and efficient sulfurizing reagent in solid phase synthesis of phosphorothioate oligodeoxyribonucleotides via the phosphoramidite approach. Product yield and quality based on IP-LC-MS compares well with high quality oligonucleotides synthesized using phenylacetyl disulfide (PADS) which is being used for manufacture of our antisense drugs.
authors.affiliation: Isis Pharmaceuticals, Carlsbad, California 92008, USA.
authors.forename: Zacharia S
authors.initials: ZS
authors.lastname: Cheruvallath
authors.forename: R Krishna
authors.initials: RK
authors.lastname: Kumar
authors.forename: Claus
authors.initials: C
authors.lastname: Rentel
authors.forename: Douglas L
authors.initials: DL
authors.lastname: Cole
authors.forename: Vasulinga T
authors.initials: VT
authors.lastname: Ravikumar
mesh_terms: D002254:Carbonates / Q000737:chemistry*; D007202:Indicators and Reagents; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D013440:Sulfides / Q000737:chemistry*; D013455:Sulfur / Q000737:chemistry*; D013873:Thionucleotides / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D002254:Carbonates; D007202:Indicators and Reagents; D009841:Oligonucleotides; D013440:Sulfides; D013873:Thionucleotides; C482610:diethyldithiodicarbonate disulfide; D013455:Sulfur