title: Pattern recognition receptors and differentiation antigens define murine myeloid cell heterogeneity ex vivo.
abstract: Recruitment of myeloid cells during inflammatory reactions plays an important role in the propagation and resolution of inflammation. However, the identification and characterization of these cells in mice has been hampered by cellular heterogeneity at the functional and phenotypic level. We have defined criteria for the rapid flow-cytometric identification of monocytes (M(o)), macrophages (MPhi), neutrophils (N(eu)) and eosinophils (E(os)) in murine tissues using novel and established myeloid markers. These criteria were applied to the study of naive mice and mice with experimentally induced inflammation, both local and systemic, and also to a murine model of tumor progression. We show that the murine 7/4 antigen and the beta-glucan receptor, Dectin-1, are particularly useful for the sub-division of myeloid cells into individual populations, even when inflammatory conditions modulate their surface expression. Furthermore, 7/4 expression allows distinction between M(o) recently recruited to a site and the resident cells already present. These studies highlight the heterogeneity of the murine M(o)/MPhi-lineage, define an extended phenotype for murine myeloid cells and greatly facilitate the ex vivo characterization of these cells during very different models of inflammation.
authors.affiliation: Sir William Dunn School of Pathology, Oxford University, Oxford, GB. ptaylor@molbiol.ox.ac.uk
authors.forename: Philip R
authors.initials: PR
authors.lastname: Taylor
authors.forename: Gordon D
authors.initials: GD
authors.lastname: Brown
authors.forename: Anja B
authors.initials: AB
authors.lastname: Geldhof
authors.forename: Luisa
authors.initials: L
authors.lastname: Martinez-Pomares
authors.forename: Siamon
authors.initials: S
authors.lastname: Gordon
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism*; D039481:CD11b Antigen / Q000378:metabolism; D004804:Eosinophils / Q000166:cytology / Q000276:immunology; D066298:In Vitro Techniques; D007249:Inflammation / Q000276:immunology / Q000473:pathology; D037181:Lectins, C-Type / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000276:immunology; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008806:Mice, Inbred AKR; D008810:Mice, Inbred C57BL; D009000:Monocytes / Q000166:cytology / Q000276:immunology; D022423:Myeloid Cells / Q000145:classification / Q000166:cytology / Q000276:immunology*; D009374:Neoplasms, Experimental / Q000276:immunology / Q000473:pathology; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009504:Neutrophils / Q000166:cytology / Q000276:immunology; D010641:Phenotype; D011956:Receptors, Cell Surface / Q000378:metabolism; D011971:Receptors, Immunologic / Q000378:metabolism*; D000099181:Dectin-1
chemical_list: D000943:Antigens, Differentiation; D039481:CD11b Antigen; D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D000099181:Dectin-1; C045268:beta-glucan receptor