[Isoenzyme analysis on different isolates of Trichomonas vaginalis].
OBJECTIVE
To study the biological types on the seven isolates of Trichomonas vaginalis from Beijing, Hebei-Tangshan, Hebei-Chengde and Jiangxi-Jiujiang in the mainland of China.


METHODS
The samples were analyzed by PAGE, isoenzyme stain and cluster analysis.


RESULTS
The isoenzyme systems used in the study included MDH, LDH, G-6-PD, PGI and PGM. No difference in the isoenzyme patterns of G-6-PD and PGI was found among the seven isolates. The MDH and LDH patterns of Beijing 1, Beijing 2, Jiujiang 3 strains were identical, while they were distinguishable from those of Chengde, Tangshan, Jiujiang 1, Jiujiang 2 isolates. The PGM pattern of Beijing 1 and Beijing 2 isolates were same but was different from that of the remainders. Gene tree was constructed according to the isoenzyme profiles. The results showed that there are differences in the patterns of the five isoenzymes between the isolates of Beijing 1, Beijing 2, Jiujiang 3 and other four isolates, and Jiujiang 3 was different from Beijing 1, Beijing 2 slightly.


CONCLUSIONS
It seems reasonable to assume that there are at least three different biological types of Trichomonas vaginalis in China.
Yuan
Li-jie
LJ
Department of Biology, North China Coal Medical College, Hebei 063000.
Gao
Xing-zheng
XZ
D000818:Animals; D002681:China; D016000:Cluster Analysis; D004591:Electrophoresis, Polyacrylamide Gel; D007527:Isoenzymes / Q000032:analysis*; D013194:Staining and Labeling; D014246:Trichomonas vaginalis / Q000145:classification / Q000201:enzymology* / Q000302:isolation & purification
D007527:Isoenzymes