Effect of tributylphenyltetraethoxylate on enzyme production of Pleurotus ostreatus.
The effect of the addition of the nonionic surfactant tributylphenyltetraethoxylate to culture media on pH and extracellular protein content, and on production of beta-glucosidase, xylanase, laccase, and manganese-dependent and -independent peroxidases by the edible fungus Pleurotus ostreatus was determined. The relationship between fermentation parameters and concentration of surfactant was assessed by multiple linear regression analysis, and the similarities and differences among the fermentation parameters were elucidated by principal component analysis. Calculations proved that except for xylanase all other cultivation parameters were significantly influenced by the surfactant, with the effect higher at higher surfactant concentrations. Surfactant increased the production of beta-glucosidase and inhibited laccase and manganese-dependent and -independent peroxidase activities.
Ramos
Cristina
C
National Agronomical Station, Quinta do Marques, 2784-505 Oeiras, Portugal.
Morais
Helena
H
Forgács
Esther
E
Cserháti
Tibor
T
D003470:Culture Media / Q000494:pharmacology; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007477:Ions; D007700:Kinetics; D042845:Laccase / Q000737:chemistry; D016014:Linear Models; D008345:Manganese / Q000737:chemistry; D010544:Peroxidases / Q000737:chemistry; D020076:Pleurotus / Q000201:enzymology*; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology*; D013501:Surface-Active Agents / Q000737:chemistry / Q000494:pharmacology*; D013997:Time Factors; D043325:Xylan Endo-1,3-beta-Xylosidase / Q000737:chemistry; D001617:beta-Glucosidase / Q000737:chemistry
D003470:Culture Media; D007477:Ions; D013501:Surface-Active Agents; C479481:tributylphenyltetraethoxylate; D011092:Polyethylene Glycols; D008345:Manganese; D042845:Laccase; D010544:Peroxidases; C051129:manganese peroxidase; D001617:beta-Glucosidase; D043325:Xylan Endo-1,3-beta-Xylosidase