[The correlation between DDVP resistance of Culex pipiens pallens and esterase activity].
OBJECTIVE
To detect the resistance index and esterase activity of each generation of DDVP-resistant Culex mosquitoes and analyze the relationship between insecticide resistance and esterase.


METHODS
WHO bioassay and micro-plate measurement were used for the detection.


RESULTS
The resistance index increased to 12.17 after 43 generations' insecticide selection compared to 1.00 as sensitive isolate. The nonspecific esterase(NSE) activity of the mosquitoes became strengthened with the extension of the generations, and the individual frequency of those with OD values no less than 0.9 increased gradually, consistent basically to the bioassay. The AChE average inhibition rate decreased with the extended generation and increased resistance, and the individual frequency of those with inhibition rate less than 30% became strengthened with the extension of generations, showing a positive correlation.


CONCLUSIONS
The activity of NSE and AChE shows a correlation with DDVP resistance.
Wang
Xin-guo
XG
Shandong Institute of Parasitic Diseases, Jining 272033.
Zhen
Tian-min
TM
Tan
Wen-bin
WB
Wang
Huai-wei
HW
Gong
Mao-qing
MQ
Sun
Chuan-hong
CH
Zhao
Yu-qiang
YQ
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D043182:Carboxylesterase / Q000378:metabolism; D003465:Culex / Q000187:drug effects / Q000201:enzymology*; D004006:Dichlorvos / Q000494:pharmacology*; D004950:Esterases / Q000378:metabolism*; D007305:Insecticide Resistance; D007306:Insecticides / Q000494:pharmacology*
D007306:Insecticides; D004006:Dichlorvos; D004950:Esterases; D043182:Carboxylesterase; D000110:Acetylcholinesterase