Non-degradative dissolution and acetylation of ball-milled plant cell walls: high-resolution solution-state NMR.
Two solvent systems for fully dissolving, and optionally derivatizing, finely ground plant cell wall material at room temperature are described: dimethylsulfoxide (DMSO) and tetrabutylammonium fluoride (TBAF) or N-methylimidazole (NMI). In situ acetylation produces acetylated cell walls (Ac-CWs) that are fully soluble in chloroform. Lignin structures tested remain fully intact. The dispersion of 13C-1H correlations afforded by two-dimensional (2D) nuclear magnetic resonance (NMR) experiments reveals the major lignin units, allowing the whole lignin fraction to be analyzed by high-resolution solution-state NMR methods for the first time. Non-degradative cell wall dissolution offers the potential to analyze polysaccharide components, and improve current cell wall analytical methods by using standard homogeneous solution-state chemistry.
Lu
Fachuang
F
U.S. Dairy Forage Research Center, USDA-Agricultural Research Service, Madison, WI 53706, USA.
Ralph
John
J
D000107:Acetylation; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism*; D004121:Dimethyl Sulfoxide / Q000378:metabolism*; D007093:Imidazoles / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D028223:Pinus; D010944:Plants / Q000737:chemistry / Q000378:metabolism*; D032107:Populus; D000644:Quaternary Ammonium Compounds / Q000378:metabolism*; D012997:Solvents / Q000378:metabolism*
D007093:Imidazoles; D000644:Quaternary Ammonium Compounds; D012997:Solvents; C009405:tetrabutylammonium; C018100:1-methylimidazole; D004121:Dimethyl Sulfoxide