DNA recognition control of gamma-PRNA and mismatched base effects upon complex stability.
Oligomeric peptide ribonucleic acids (PRNA's), tethering 5'-amino-5'-deoxyribonucleosides as a recognition site for nucleic acids, has been designed and synthesized by solid phase method. PRNA 12-mer found to form stable duplex with complementary DNA.PRNA.DNA duplex composed of PRNA with mismatched DNA has 10-degree lower melting temperature than fullmatched PRNA.DNA duplex. These results demonstrate that binding of PRNA with DNA is sequence specific. It should be emphasized that the on-off switching of PRNA.DNA duplex formation has been realized by the borate added as an external factor.
Sato
Hirofumi
H
Department of Molecular Chemistry, Graduate School of Engineering, Osaka University, Yamada-oka, Suita 565-0871, Japan.
Hashimoto
Yusuke
Y
Kikkawa
Mayuko
M
Wada
Takehiko
T
Inoue
Yoshihisa
Y
D020137:Base Pair Mismatch; D001483:Base Sequence; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D020135:Peptide Nucleic Acids / Q000737:chemistry*
D020135:Peptide Nucleic Acids; D004247:DNA