title: Taenia solium metacestode antigens which are protective for pigs induce Th1/Th2 mixed responses in mice.
abstract: The purpose of this study was to determine the Th1 and Th2 cytokine responses induced by Taenia solium metacestode antigens in mice and correlate them with the immune responses elicited in vivo. To assess this aim, mice were inoculated with metacestode antigens. RNA was obtained from spleen cells of immunized or control mice incubated with metacestode antigens and used to determine the cytokine profile. Peripheral blood eosinophilia was measured daily in each mouse and specific serum antibody levels were determined. Results showed that metacestode antigens induce the synthesis of IL-4, IL-5 and IFN-gamma mRNAs in spleen cells. They also induced peripheral blood eosinophilia and elicited specific IgE and IgG antibodies, especially IgG1. Three antigens were recognized by all IgG subclasses and by IgE (104, 88 and 7 kDa), and a 57-kDa protein was recognized by IgG1, IgG2a, IgG2b, and IgE. IgG1 and IgG2b recognized 52, 30 and 20 kDa antigens. Immune responses elicited in vivo and the cytokine profile showed good correlation.
authors.affiliation: Department of Microbiology and Parasitology, Faculty of Medicine, National Autonomous University of Mexico, Av. Universidad 3000, 04510 Mexico City, Mexico.
authors.forename: Isabel
authors.initials: I
authors.lastname: Cortes
authors.forename: Jose Luis
authors.initials: JL
authors.lastname: Molinari
authors.forename: Sandra
authors.initials: S
authors.lastname: Solano
authors.forename: Lilian
authors.initials: L
authors.lastname: Hernandez-Mendoza
authors.forename: Antonio
authors.initials: A
authors.lastname: Ramirez
authors.forename: Patricia
authors.initials: P
authors.lastname: Tato
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000008:administration & dosage* / Q000737:chemistry / Q000276:immunology; D003551:Cysticercosis / Q000469:parasitology / Q000662:veterinary; D016207:Cytokines / Q000096:biosynthesis*; D004802:Eosinophilia; D005260:Female; D007114:Immunization; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007132:Immunoglobulin Isotypes / Q000097:blood; D007700:Kinetics; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D013552:Swine; D013553:Swine Diseases / Q000469:parasitology / Q000517:prevention & control*; D041201:Taenia solium / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D016207:Cytokines; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D007073:Immunoglobulin E