title: Resistin is expressed in different rat tissues and is regulated in a tissue- and gender-specific manner.
abstract: Resistin is a polypeptide hormone first reported from human and rodent adipocytes. In order to better define the potential biological role of resistin we undertook a detailed analysis of its expression in different rat tissues. We demonstrate by real-time reverse transcription polymerase chain reaction, Southern blotting and immunohistochemistry that resistin is expressed not only in brown and white adipose tissue, but also in the stomach, small and large intestines, adrenal gland, and skeletal muscle. Food deprivation led to a decrease in resistin mRNA expression only in adipose tissue, not in any of the other tissues studied. Furthermore, resistin mRNA expression is higher in males than in females in adipose tissue, not in any of the other tissues. Thus, our data suggest that resistin is not exclusively localized in adipocytes, and indicate that its expression is regulated in a tissue- and sex-specific manner.
authors.affiliation: Department of Physiology, University of Santiago de Compostela, School of Medicine, 15705, Santiago de Compostela, Spain
authors.forename: Rubén
authors.initials: R
authors.lastname: Nogueiras
authors.forename: Rosalía
authors.initials: R
authors.lastname: Gallego
authors.forename: Oreste
authors.initials: O
authors.lastname: Gualillo
authors.forename: J Eduardo
authors.initials: JE
authors.lastname: Caminos
authors.forename: Tomás
authors.initials: T
authors.lastname: García-Caballero
authors.forename: Felipe F
authors.initials: FF
authors.lastname: Casanueva
authors.forename: Carlos
authors.initials: C
authors.lastname: Diéguez
mesh_terms: D000818:Animals; D005260:Female; D005508:Food Deprivation; D005786:Gene Expression Regulation; D006729:Hormones, Ectopic / Q000378:metabolism*; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins; D008297:Male; D020932:Nerve Growth Factor; D009928:Organ Specificity; D011506:Proteins; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D052243:Resistin; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012737:Sex Factors; D012746:Sexual Abstinence; D014018:Tissue Distribution
chemical_list: D006729:Hormones, Ectopic; D036341:Intercellular Signaling Peptides and Proteins; D011506:Proteins; C486574:RETNLB protein, human; D012333:RNA, Messenger; D052243:Resistin; C499622:Retn protein, rat; C486575:Retnla protein, rat; D020932:Nerve Growth Factor