An unnatural base pair between imidazolin-2-one and 2-amino-6-(2-thienyl)purine in replication and transcription.
Nucleosides of imidazolin-2-one (designated by z) were designed and synthesized as pairing partners of 2-amino-6-(2-thienyl)purine (designated by s). Previously, we developed an unnatural base pair between s and pyridine-2-one (designated by y), and polymerases specifically incorporated the substrate of y into DNA and RNA opposite s in templates. Although s was efficiently incorporated opposite y, A was also incorporated opposite y with high efficiency. The replacement of y by z effectively improved the incorporation selectivity of s. The incorporation efficiency of A opposite z decreased, but the efficient incorporation of s opposite z was maintained as compared to that of the s-y pairing.
Hirao
Ichiro
I
Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
Kimoto
Michiko
M
Mitsui
Tsuneo
T
Harada
Yoko
Y
Fujiwara
Tsuyoshi
T
Sato
Akira
A
Yokoyama
Shigeyuki
S
D020029:Base Pairing; D004261:DNA Replication; D007093:Imidazoles / Q000737:chemistry*; D011687:Purines / Q000737:chemistry*; D014158:Transcription, Genetic
C435686:2-amino-6-(2-thienyl)purine; D007093:Imidazoles; D011687:Purines