Transfer of pro-R hydrogen from NADH to dihydroxyacetonephosphate by sn-glycerol-1-phosphate dehydrogenase from the archaeon Methanothermobacter thermautotrophicus.
sn-Glycerol-1-phosphate dehydrogenase is responsible for the formation of the sn-glycerol-1-phosphate backbone of archaeal lipids. [4-3H]NADH that had 3H at the R side was produced from [4-3H]NAD and glucose with glucose dehydrogenase (a pro-S type enzyme). The 3H of this [4-3H]NADH was transferred to dihydroxyacetonephosphate during the sn-glycerol-1-phosphate dehydrogenase reaction. On the contrary, in a similar reaction using alcohol dehydrogenase (a pro-R type enzyme), 3H was not incorporated into glycerophosphate. These results confirmed a prediction of the tertiary structure of sn-glycerol-1-phosphate dehydrogenase by homology modeling.
Koga
Yosuke
Y
Department of Chemistry, University of Occupational and Environmental Health, Kitakyushu, Japan. kogay@med.uoeh-u.ac.jp
Sone
Nobuhito
N
Noguchi
Shunsuke
S
Morii
Hiroyuki
H
D004099:Dihydroxyacetone Phosphate / Q000378:metabolism*; D005993:Glycerolphosphate Dehydrogenase / Q000737:chemistry / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D017013:Methanobacteriaceae / Q000201:enzymology*; D008968:Molecular Conformation; D009243:NAD / Q000378:metabolism*
D009243:NAD; D004099:Dihydroxyacetone Phosphate; D006859:Hydrogen; D005993:Glycerolphosphate Dehydrogenase; C098215:glycerol-1-phosphate dehydrogenase