title: A novel strategy for analysis of gene homologues and segmental genome duplications.
abstract: Transformation-associated recombination (TAR) cloning allows selective isolation of a desired chromosomal region or gene from complex genomes. The method exploits a high level of recombination between homologous DNA sequences during transformation in the yeast Saccharomyces cerevisiae. We investigated the effect of nonhomology on the efficiency of gene capture and found that up to 15% DNA divergence did not prevent efficient gene isolation. Such tolerance to DNA divergence greatly expands the potential applications of TAR cloning for comparative genomics. In this study, we were able to use the technique to isolate nonidentical chromosomal duplications and gene homologues.
authors.affiliation: Laboratory of Biosystems and Cancer, Center for Cancer Research, National Cancer Institute, NIH, Building 37, Room 5032, Bethesda, MD 20892, USA.
authors.forename: Vladimir N
authors.initials: VN
authors.lastname: Noskov
authors.forename: Sun-Hee
authors.initials: SH
authors.lastname: Leem
authors.forename: Greg
authors.initials: G
authors.lastname: Solomon
authors.forename: Michael
authors.initials: M
authors.lastname: Mullokandov
authors.forename: Ji-Youn
authors.initials: JY
authors.lastname: Chae
authors.forename: Young-Ho
authors.initials: YH
authors.lastname: Yoon
authors.forename: Young-Sun
authors.initials: YS
authors.lastname: Shin
authors.forename: Natalay
authors.initials: N
authors.lastname: Kouprina
authors.forename: Vladimir
authors.initials: V
authors.lastname: Larionov
mesh_terms: D002503:Centromere / Q000235:genetics; D022202:Chromosomes, Artificial, Bacterial; D002888:Chromosomes, Human, Pair 19; D003001:Cloning, Molecular / Q000379:methods; D020440:Gene Duplication; D016678:Genome; D015894:Genome, Human; D006801:Humans; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics; D016415:Sequence Alignment / Q000379:methods*; D012689:Sequence Homology, Nucleic Acid; D014170:Transformation, Genetic