Microphysiological testing for chemosensitivity of living tumor cells with multiparametric microsensor chips.
A constraint in the reliability of predictive chemosensitivity assays is linked to the fact that they analyze only a single cellular or biochemical parameter. A multiparametric test system using microsensor chips has been developed which can detect online microphysiological changes in living cells. Tumor cells were grown directly on glass- or silicon-based electronic sensor chips. Changes in extracellular pH and pO(2), reflecting metabolic activities, and changes in impedance, reflecting morphological properties, were monitored. In this study, colon and breast cancer cells as well as doxorubicin-sensitive and doxorubicin-resistant sarcoma cell lines were exposed to cytochalasin B, chloroacetaldehyde, or doxorubicin. Results show (1) reduction in medium acidification, (2) marked and rapid changes in O(2) consumption, and (3) modulations in impedance correlating with morphological changes observed in the microscope. Drug-resistant cells do not show these changes. Therefore, this microphysiological monitoring is a versatile tool for chemosensitivity testing of tumor cells.
Otto
Angela M
AM
Heinz-Nixdorf-Chair of Medical Electronics, Technical University of Munich, Arcisstrasse 21, D-80333, Munich, Germany. angela.otto@ei.tum.de
Brischwein
Martin
M
Niendorf
Axel
A
Henning
Tobias
T
Motrescu
Elena
E
Wolf
Bernhard
B
D000970:Antineoplastic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D019008:Drug Resistance, Neoplasm; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008962:Models, Theoretical; D036103:Nanotechnology / Q000295:instrumentation; D010101:Oxygen Consumption; D015203:Reproducibility of Results; D012509:Sarcoma / Q000378:metabolism / Q000473:pathology*; D012983:Soft Tissue Neoplasms / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents