Development of a novel functional biosensor with a short Ca(2+)-dependent deoxyribozyme.
We develop a novel functional biosensor on a deoxyribozyme. A 5'-end-immobilized short Ca(2+)-dependent deoxyribozyme (dCGCTGGCAGGCTACAACGAGTCTTC) binds to a target RNA substrate (rGAAGACA decrease UGCCAGCG; decrease denotes an RNA cleavage site), and acts as an enzyme in the presence of Ca2+. It cleaves the target RNA substrate at one site of rAp decrease U in the asymmetric internal loop.
Okumoto
Y
Y
Department of Chemistry, Faculty of Science, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan.
Sugimoto
N
N
D001483:Base Sequence; D001665:Binding Sites; D015374:Biosensing Techniques / Q000379:methods*; D002118:Calcium / Q000378:metabolism; D021881:DNA, Catalytic / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D009690:Nucleic Acid Conformation; D012313:RNA / Q000235:genetics / Q000378:metabolism
D021881:DNA, Catalytic; D012313:RNA; D002118:Calcium