Expression of extracellular matrix metalloprotease inducer in laryngeal squamous cell carcinoma.
OBJECTIVE
Head and neck cancer tumor cell invasion is responsible for both local destruction and distant metastasis. Invasion is largely mediated by matrix metalloproteases that are thought to be induced by tumor cell derived extracellular matrix metalloprotease inducer (EMMPRIN) in surrounding fibroblasts. We hypothesize that EMMPRIN is overexpressed in laryngeal cancer.


METHODS
Retrospective analysis of patients with supraglottic laryngeal cancer.


METHODS
Total protein immunoblotting and immunohistochemical analysis of normal and malignant tissue were performed to determine EMMPRIN expression. EMMPRIN immunoreactivity in 33 patients was correlated with clinicopathological features and survival.


RESULTS
Whole-tissue lysates of tumors (n = 8) and metastatic lymph nodes (n = 2), but not normal skin (n = 8) or mucosa (n = 6), expressed significant amounts of EMMPRIN by immunoblotting. EMMPRIN membrane immunoreactivity (transmembrane EMMPRIN score) was associated with nodal positivity (P =.07), and it was a borderline significant predictor of survival (Hazards Ratio = 2.4; 95% CI, 0.88-6.55). As a categorical variable, higher transmembrane EMMPRIN score was associated with higher mortality.


CONCLUSIONS
The present study helps to establish EMMPRIN as a widely expressed protein in dysplastic mucosa and supraglottic laryngeal cancer, but not in normal epithelial counterparts.
Rosenthal
Eben L
EL
Division of Otolarynology-Head and Neck Surgery, Comprehensive Cancer Center, University of Alabama at Birmingham, 35249, USA. oto@uab.edu
Shreenivas
Satya
S
Peters
Glenn E
GE
Grizzle
William E
WE
Desmond
Renee
R
Gladson
Candece L
CL
D015703:Antigens, CD; D000951:Antigens, Neoplasm; D051926:Basigin; D014408:Biomarkers, Tumor / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000737:chemistry*; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D007822:Laryngeal Neoplasms / Q000737:chemistry*; D007830:Larynx / Q000737:chemistry; D008207:Lymphatic Metastasis; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis*; D008875:Middle Aged; D009092:Mucous Membrane / Q000737:chemistry; D012189:Retrospective Studies; D012867:Skin / Q000737:chemistry
D015703:Antigens, CD; D000951:Antigens, Neoplasm; C497886:BSG protein, human; D014408:Biomarkers, Tumor; D008562:Membrane Glycoproteins; D051926:Basigin