title: Effect of linezolid on the phagocytic functions of human polymorphonuclear leukocytes.
abstract: The effect of linezolid on the phagocytic and bactericidal functions of human polymorphonuclear neutrophils (PMN) against gram-positive cocci was evaluated. Preincubation (30 min; 37 degrees C) of PMN with different concentrations of linezolid (2, 10 and 20 mg/l) did not significantly affect the phagocytosis of either Staphylococcus aureus (methicillin susceptible and resistant) or Enterococcus faecalis (vancomycin susceptible and resistant). Overnight exposure of vancomycin-resistant E. faecalis to 1/4 minimum inhibitory concentrations (MIC) of linezolid slightly increased phagocytosis by PMN. Preincubation of the other three strains with 1/4 MIC of linezolid did not affect phagocytosis by these cells. Preincubation of PMN (30 min; 37 degrees C) using different extracellular concentrations of linezolid (2, 10 and 20 mg/l) did not affect their production of either superoxide or hydrogen peroxide radicals. In conclusion, linezolid did not affect the phagocytic and bactericidal functions of human PMN.
authors.affiliation: Department of Microbiology, University of Seville School of Medicine, Seville, Spain.
authors.forename: Sofía
authors.initials: S
authors.lastname: Ballesta
authors.forename: Alvaro
authors.initials: A
authors.lastname: Pascual
authors.forename: Isabel
authors.initials: I
authors.lastname: García
authors.forename: Evelio J
authors.initials: EJ
authors.lastname: Perea
mesh_terms: D000081:Acetamides / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001770:Blood Bactericidal Activity / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D013293:Enterococcus faecalis / Q000276:immunology; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D066298:In Vitro Techniques; D000069349:Linezolid; D016106:Methicillin Resistance; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology / Q000382:microbiology; D023303:Oxazolidinones / Q000494:pharmacology*; D010587:Phagocytosis / Q000187:drug effects*; D013211:Staphylococcus aureus / Q000276:immunology; D013481:Superoxides / Q000378:metabolism; D014640:Vancomycin / Q000494:pharmacology; D020713:Vancomycin Resistance
chemical_list: D000081:Acetamides; D000900:Anti-Bacterial Agents; D023303:Oxazolidinones; D013481:Superoxides; D014640:Vancomycin; D006861:Hydrogen Peroxide; D000069349:Linezolid