title: [Expression of cyclooxygenase-2 mRNA and identification of its spliceriant in human myometrium obtained from women in labor].
abstract: OBJECTIVE
  To investigate the expression of cyclooxygenase-2 (COX-2) in human lower segments of myometrium obtained from women in labor and those not in labor and identify the splice variant of COX-2.
  
  
  METHODS
  Reverse transcriptase-polymerase chain reaction (RT-PCR) was used for investigating the expression of COX-2. According to the sequence of rat COX-2 splice variant, the primers were designed and synthesized, then the splice variant of COX-2 in human myometrium from woman in labor was identified, cloned into vector and sequenced.
  
  
  RESULTS
  The results showed that (1) The Expression of COX-2 mRNA was lower in human myometrium obtained from women who were not in labor than those in labor. (2) A new band of COX-2 was obtained in myometrium from a woman in labor. The fragment includes an unspliced intron, which locates between exons 7 and 8.
  
  
  CONCLUSIONS
  COX-2 gene is not only expressed highly in human myometrium from women in labor, but also produced splicing variant by alternative splicing.
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China.
authors.forename: Yin-ping
authors.initials: YP
authors.lastname: Huang
authors.forename: Du-yun
authors.initials: DY
authors.lastname: Ye
authors.forename: Ping
authors.initials: P
authors.lastname: Wu
authors.forename: Yun-feng
authors.initials: YF
authors.lastname: Huang
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.forename: Hong-gang
authors.initials: HG
authors.lastname: Ying
authors.forename: Qian
authors.initials: Q
authors.lastname: Pan
authors.forename: Jing-yuan
authors.initials: JY
authors.lastname: Wan
authors.forename: Ping
authors.initials: P
authors.lastname: Yuan
authors.forename: Dai-juan
authors.initials: DJ
authors.lastname: Zhang
mesh_terms: D000328:Adult; D017398:Alternative Splicing / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D051546:Cyclooxygenase 2; D018076:DNA, Complementary; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D007743:Labor, Obstetric / Q000378:metabolism*; D008565:Membrane Proteins; D009215:Myometrium / Q000201:enzymology*; D011247:Pregnancy; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018076:DNA, Complementary; D007527:Isoenzymes; D008565:Membrane Proteins; D012333:RNA, Messenger; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases