[Acanthamoeba isolated from child thymus: Acanthamoeba growth during cocultivation with human and murine eukaryotic cells and induction of cytopathogenic effect on these cells].
A cell line of the ameba assigned to the genus Acanthamoeba by morphological and cariotypic signs and by the specific features of its life cycle was isolated from the thymus of a child operated on for heart disease. The line received the name CDHT (Cells Derived from Human Thymus). Actively proliferating mammalian cells maintain the multiplication of Acanthamoeba cells in vitro. Using thymocytes as an example, it was shown that death of these cells occurred through the mechanism of apoptosis. The human thymocytes preincubated with the supernatant obtained through the cocultivation of CDHT and thymocytes may also undergo apoptosis.
Komogorova
V V
VV
Sharova
N I
NI
Gordeeva
L M
LM
Iarilin
A A
AA
D000048:Acanthamoeba / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D000818:Animals; D017209:Apoptosis; D002460:Cell Line; D002648:Child; D018920:Coculture Techniques; D017077:Culture Media, Conditioned; D005057:Eukaryotic Cells / Q000473:pathology*; D006801:Humans; D051379:Mice; D013950:Thymus Gland / Q000166:cytology / Q000469:parasitology*; D013997:Time Factors
D017077:Culture Media, Conditioned