Adhesive resin induces apoptosis and cell-cycle arrest of pulp cells.
The application of an adhesive resin near or directly over the pulp was shown to induce pulp inflammation and lack of dentin regeneration. We hypothesize that the absence of dentin bridging is due to adhesive-resin-induced apoptosis of cells responsible for pulp healing and dentin regeneration. Mouse odontoblast-like cells (MDPC-23), undifferentiated pulp cells (OD-21), or macrophages (RAW 264.7) were exposed to SingleBond polymerized for 0-40 seconds. Annexin V and propidium iodide assays demonstrated that SingleBond induced apoptosis of MDPC-23, OD-21, and macrophages. The proportion of apoptotic cells was dependent on the degree of adhesive resin polymerization. Adhesive-resin-induced death of pulp cells was associated with activation of the pro-apoptotic cysteine protease Caspase-3. Interestingly, most cells exposed to adhesive resin that did not undergo apoptosis showed cell-cycle arrest. We conclude that an adhesive resin induces apoptosis and cell-cycle arrest of cells involved in the regeneration of the dentin-pulp complex in vitro.
Mantellini
M G
MG
Department of Cariology, Restorative Sciences, and Endodontics, University of Michigan School of Dentistry, 1011 N. University, Rm. 5211, Ann Arbor, MI 48109-1078, USA.
Botero
T M
TM
Yaman
P
P
Dennison
J B
JB
Hanks
C T
CT
Nör
J E
JE
D000704:Analysis of Variance; D000818:Animals; D017304:Annexin A5 / Q000032:analysis; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D020169:Caspases / Q000032:analysis; D018929:Cell Culture Techniques; D002453:Cell Cycle / Q000187:drug effects; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects*; D017220:Dentin-Bonding Agents / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000032:analysis; D004792:Enzyme Precursors / Q000032:analysis; D007202:Indicators and Reagents; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D009804:Odontoblasts / Q000187:drug effects; D011419:Propidium
D017304:Annexin A5; D017220:Dentin-Bonding Agents; D004791:Enzyme Inhibitors; D004792:Enzyme Precursors; D007202:Indicators and Reagents; D011419:Propidium; C505409:Casp3 protein, mouse; D053148:Caspase 3; D020169:Caspases