title: Antimicrobial constituents from goldenseal (the Rhizomes of Hydrastis canadensis) against selected oral pathogens.
abstract: Two new C-methyl flavonoids, 6,8-di- C-methylluteolin 7-methyl ether (1) and 6- C-methylluteolin 7-methyl ether (2), were isolated from a commercially available sample of the roots of Hydrastis canadensis, along with seven known compounds, berberine (3), beta-hydrastine (4), canadine (5), canadaline (6), isocorypalmine (7), canadinic acid (8), and beta-sitosterol 3- O-beta- D-glucoside (9). The structures of the new compounds 1 and 2 were elucidated on the basis of their spectral data including 1D and 2D NMR techniques. Of these isolates, berberine (3) and, to a lesser extent, 1 and 2, showed antimicrobial activity when evaluated against the oral pathogens Streptococcus mutans and Fusobacterium nucleatum. Berberine (3) exhibited an additive antimicrobial effect when tested against S. mutans in combination with 1.
authors.affiliation: Program for Collaborative Research in the Pharmaceutical Sciences, College of Pharmacy, University of Illinois at Chicago, Chicago, IL, USA.
authors.forename: Bang Yeon
authors.initials: BY
authors.lastname: Hwang
authors.forename: Sara K
authors.initials: SK
authors.lastname: Roberts
authors.forename: Lucas R
authors.initials: LR
authors.lastname: Chadwick
authors.forename: Christine D
authors.initials: CD
authors.lastname: Wu
authors.forename: A Douglas
authors.initials: AD
authors.lastname: Kinghorn
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology; D005673:Fusobacterium / Q000187:drug effects; D039321:Hydrastis / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D009055:Mouth / Q000382:microbiology; D027343:Rhizome / Q000737:chemistry; D013295:Streptococcus mutans / Q000187:drug effects
chemical_list: C477676:6-C-methylluteolin 7-methyl ether; D000900:Anti-Bacterial Agents; D005419:Flavonoids