The complete nucleotide sequence of two distinct geminiviruses infecting cucurbits in Vietnam.
We have characterised two distinct geminiviruses that infect cucurbit cultivars in Vietnam. The genomes of both viruses consisted of two circular ssDNA components (DNA-A and DNA-B), with a genome arrangement and coding sequence typical of viruses in the Begomovirus genus in the family Geminiviridae. The sequence of DNA-A of one of the viruses was approximately 97% similar to Squash leaf curl virus-China (SLCV-Ch), for which a DNA-B has yet to be identified. We have named this virus Squash leaf curl virus-Vietnam (SLCV-Vn). The intergenic region of the SLCV-Vn DNA-B contained a 40 nt deletion between the putative AC1 TATA box and the stem loop. A second virus isolated from loofa in southern Vietnam was only 80% similar to SLCV-Vn over the complete DNA-A sequence, however the nucleotide sequence in the coat protein coding regions was 95% similar. We have named this virus Loofa yellow mosaic virus-Vietnam (LYMV-Vn). Other regions of the SLCV-Vn and LYMV-Vn genomes differed markedly, suggesting the coat protein coding region was recombinant. The DNA-B of both viruses were only 60% similar over the complete nucleotide sequence, although the encoded amino acid sequence of the BC1 gene was 90% identical.
Revill
P A
PA
Plant Biotechnology, Science Research Centre, Queensland University of Technology, Brisbane, Australia. p.revill@qut.edu.au
Ha
C V
CV
Porchun
S C
SC
Vu
M T
MT
Dale
J L
JL
D001483:Base Sequence; D028464:Cucurbita / Q000821:virology*; D021901:DNA, Intergenic / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D017791:Geminiviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014644:Genetic Variation; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D018515:Plant Leaves / Q000821:virology; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D014744:Vietnam
D021901:DNA, Intergenic; D004279:DNA, Viral