Crystallization of the most active RNA-cleaving deoxyribozyme.
The structural compositions of the most active deoxyribozyme and its derivatives have been examined by electrophoresis, and their crystallization conditions were surveyed for X-ray analysis. It has been found that Mg2+ ion is essential to form the active binary complex between the catalytic DNA and the substrate, and that heat-treatment is effective to prevent formation of the inactive quaternary complex between the two enzymes and the two substrates. Crystals obtained by the hanging drop vapor diffusion method are composed of the active binary complex.
Kondo
J
J
Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama, Kanagawa 226-8501, Japan.
Takénaka
A
A
D001483:Base Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D021881:DNA, Catalytic / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D066298:In Vitro Techniques; D008274:Magnesium / Q000378:metabolism; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity
D021881:DNA, Catalytic; D012313:RNA; D008274:Magnesium