title: The VirD2 pilot protein of Agrobacterium-transferred DNA interacts with the TATA box-binding protein and a nuclear protein kinase in plants.
abstract: The bacterial virulence protein VirD2 plays an important role in nuclear import and chromosomal integration of Agrobacterium-transferred DNA in fungal, plant, animal, and human cells. Here we show that in nuclei of alfalfa cells, VirD2 interacts with and is phosphorylated by CAK2Ms, a conserved plant ortholog of cyclin-dependent kinase-activating kinases. CAK2Ms binds to and phosphorylates the C-terminal regulatory domain of RNA polymerase II largest subunit, which can recruit the TATA box-binding protein. VirD2 is found in tight association with the TATA box-binding protein in vivo. These results indicate that recognition of VirD2 is mediated by widely conserved nuclear factors in eukaryotes.
authors.affiliation: Max Planck Institute for Plant Breeding Research, Carl-von-Linne-Weg 10, D-50829 Cologne (Köln), Germany.
authors.forename: László
authors.initials: L
authors.lastname: Bakó
authors.forename: Masaaki
authors.initials: M
authors.lastname: Umeda
authors.forename: Antonio F
authors.initials: AF
authors.lastname: Tiburcio
authors.forename: Jeff
authors.initials: J
authors.lastname: Schell
authors.forename: Csaba
authors.initials: C
authors.lastname: Koncz
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D002467:Cell Nucleus / Q000201:enzymology; D004269:DNA, Bacterial / Q000235:genetics*; D000455:Medicago sativa; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D010944:Plants / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology*; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012231:Rhizobium / Q000235:genetics* / Q000472:pathogenicity; D017386:Sequence Homology, Amino Acid; D035181:TATA-Box Binding Protein / Q000235:genetics / Q000378:metabolism*; D014774:Virulence; D037521:Virulence Factors
chemical_list: D029681:Arabidopsis Proteins; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D010940:Plant Proteins; D011994:Recombinant Proteins; C036483:T-DNA; D035181:TATA-Box Binding Protein; D037521:Virulence Factors; D011494:Protein Kinases; C471231:CAK2 protein, Arabidopsis