title: High resolution sequence-based DPB1 typing identified two novel DPB1 alleles, DPB1*9401 and DPB1*9501, from a Kenyan population.
abstract: Two novel DPB1 alleles, DPB1*9401 and DPB1*9501, were identified from a Kenyan population during sequence-based HLA-DPB1 typing. Molecular cloning and sequencing of multiple clones confirmed that one of the new DPB1 alleles is identical to DPB1*0402 at exon 2 except for a single nucleotide substitution (CGG -->TGG), changing codon 70 from Arg to Trp. The new allele has been named DPB1*9401. This is the first report of polymorphism at codon 70 of HLA-DPB1 alleles. New codon combinations have been identified in another novel DPB1 allele named DPB1*9501. The extensive diversity at DPB1 locus of this East African population is being revealed by high resolution sequence-based DPB1 typing.
authors.affiliation: Department of Medical Microbiology, University of Manitoba, Winnipeg, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Luo
authors.forename: S
authors.initials: S
authors.lastname: Ramdahin
authors.forename: S
authors.initials: S
authors.lastname: Iqbal
authors.forename: Y
authors.initials: Y
authors.lastname: Pan
authors.forename: K
authors.initials: K
authors.lastname: Jacobson
authors.forename: M J
authors.initials: MJ
authors.lastname: Narayansingh
authors.forename: M
authors.initials: M
authors.lastname: Schroeder
authors.forename: R C
authors.initials: RC
authors.lastname: Brunham
authors.forename: J
authors.initials: J
authors.lastname: Embree
authors.forename: F A
authors.initials: FA
authors.lastname: Plummer
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D006682:HLA-DP Antigens / Q000235:genetics*; D059868:HLA-DP beta-Chains; D006801:Humans; D007630:Kenya; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
chemical_list: D006682:HLA-DP Antigens; D059868:HLA-DP beta-Chains; C071170:HLA-DPB1 antigen