title: The relationship between the N-glycosylation of acetylglucosaminyltransferase V and its activity.
abstract: OBJECTIVE
  The goal of this paper is to investigate the relationship between the N-glycosylation of acetylglucosaminyltransferase V (GlcNAcT-V) and its activity and to know which site among the 6 N-glycosylation sites in the GlcNAcT-V gene is the most important.
  
  
  METHODS
  Wild type of GlcNAcT-V was transfected into COS-7 cells and its activity was measured 48 h later. The first site (Asn 110) was mutated with site-directed mutagenesis and transfected into COS-7 cells.
  
  
  RESULTS
  It was found that after the cells were added tunicamycin (TM, 1 microg/ml), the activity was 11.7% of the wild type. The activity of the cells with mutating GlcNAcT-V was about 12.0% of the wild type. RT-PCR showed that there was no significant change in mRNA expression among the three groups.
  
  
  CONCLUSIONS
  The N-glycosylation is important for its activity. Our results suggest that the N-linked carbohydrates on GlcNAcT-V are required for the posttranscriptional activity of the enzyme.
authors.affiliation: Shanghai Institute of Biochemistry, Chinese Academy of Sciences, Shanghai 200031.
authors.forename: Z
authors.initials: Z
authors.lastname: Zhibing
authors.forename: G
authors.initials: G
authors.lastname: Huabei
authors.forename: W
authors.initials: W
authors.lastname: Chen
authors.forename: X
authors.initials: X
authors.lastname: Feng
authors.forename: C
authors.initials: C
authors.lastname: Huili
authors.forename: J
authors.initials: J
authors.lastname: Youxin
mesh_terms: D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D006031:Glycosylation; D016297:Mutagenesis, Site-Directed; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D014415:Tunicamycin / Q000494:pharmacology
chemical_list: D012333:RNA, Messenger; D014415:Tunicamycin; D017351:N-Acetylglucosaminyltransferases; C052254:alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase