title: Simple two-state protein folding kinetics requires near-levinthal thermodynamic cooperativity.
abstract: Simple two-state folding kinetics of many small single-domain proteins are characterized by chevron plots with linear folding and unfolding arms consistent with an apparent two-state description of equilibrium thermodynamics. This phenomenon is hereby recognized as a nontrivial heteropolymer property capable of providing fundamental insight into protein energetics. Many current protein chain models, including common lattice and continuum Gō models with explicit native biases, fail to reproduce this generic protein property. Here we show that simple two-state kinetics is obtainable from models with a cooperative interplay between core burial and local conformational propensities or an extra strongly favorable energy for the native structure. These predictions suggest that intramolecular recognition in real two-state proteins is more specific than that envisioned by common Gō-like constructs with pairwise additive energies. The many-body interactions in the present kinetically two-state models lead to high thermodynamic cooperativity as measured by their van't Hoff to calorimetric enthalpy ratios, implying that the native and denatured conformational populations are well separated in enthalpy by a high free-energy barrier. It has been observed experimentally that deviations from Arrhenius behavior are often more severe for folding than for unfolding. This asymmetry may be rationalized by one of the present modeling scenarios if the effective many-body cooperative interactions stabilizing the native structure against unfolding is less dependent on temperature than the interactions that drive the folding kinetics.
authors.affiliation: Protein Engineering Network of Centres of Excellence, Department of Biochemistry, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada.
authors.forename: Hüseyin
authors.initials: H
authors.lastname: Kaya
authors.forename: Hue Sun
authors.initials: HS
authors.lastname: Chan
mesh_terms: D000465:Algorithms; D007700:Kinetics; D008956:Models, Chemical; D011487:Protein Conformation; D017510:Protein Folding; D011506:Proteins / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D011506:Proteins