title: The transmembrane signal transduction in HEp-2 cells induced by bacterial adherence.
abstract: In order to understand the role of transmembrane signal transduction of host cells in the early steps of infection, the adherence of E. coli to HEp-2 cells and the change of activity of phospholipase C-gamma (PLC-gamma) induced by the adherence were investigated. The adherence of enteropathogenic E. coli (EPEC), strain E. 7, induced a significant increase of inositol-triphosphat (IP-3) level in HEp-2 cells. The adherence of the bacteria and the increase of IP-3 was kinetically correlated. Whereas the increase of IP3 level induced by the adherence of the control strain EPEC (H511), a non-piliated strain, was much meager than that by E7, a piliated strain. The results highlighted an important role of transmembrane signals like 1P-3 in the pathogenesis of EPEC.
authors.affiliation: Department of Etiology, Institute of Basic Medical Sciences, CAMS & PUMC, Beijing 100005.
authors.forename: Y
authors.initials: Y
authors.lastname: Guan
authors.forename: L
authors.initials: L
authors.lastname: Xue
authors.forename: C
authors.initials: C
authors.lastname: Ye
authors.forename: D
authors.initials: D
authors.lastname: Zhang
mesh_terms: D001422:Bacterial Adhesion; D004926:Escherichia coli / Q000502:physiology*; D010861:Fimbriae, Bacterial / Q000502:physiology*; D007295:Inositol Phosphates / Q000378:metabolism; D007822:Laryngeal Neoplasms; D051966:Phospholipase C gamma; D015398:Signal Transduction; D014407:Tumor Cells, Cultured; D010738:Type C Phospholipases / Q000378:metabolism*
chemical_list: D007295:Inositol Phosphates; C092449:inositol 1,2,3-trisphosphate; D010738:Type C Phospholipases; D051966:Phospholipase C gamma