Endogenous heme oxygenase/carbon monoxide system mediates lipopolysaccharide-induced intussusception in rats.
OBJECTIVE
To investigate the role of endogenous heme oxygenase (HO)/carbon monoxide (CO) system in regulating the process of intussusception (IN) induced by administration of lipopolysaccharide (LPS) in rats.


METHODS
IN model of rats were induced by lipopolysaccharide. HO activity was determined by the amount of bilirubin formation which was measured with a double-beam spectrophotometer, and HbCO formation was measured by CO-oximeter.


RESULTS
The results showed that LPS (10 mg/kg) caused IN in up to 40% of the rats at 6 h after each treatment of LPS. The incidence of IN were significantly increased by 50% (P < 0.05) and by 83.2% (P < 0.01) in HO substrate (heme-L-lysinate)-treated rats and in exogenous CO-treated rats, respectively; but it was sigificantly decreased by 41.8% (P < 0.05) after administration of ZnDPBG, an inhibitor of heme oxygense (HO) activity. Furthermore, LPS increased HO activity, HbCO formation cGMP content within colic smooth muscle and the plasma level of cGMP, and these parameters were significantly elevated by 62.6% (P < 0.01), 40.0% (P < 0.01), 49.3% (P < 0.05) and 38.9% (P<0.05), respectively, compared with LPS-non-IN rats.


CONCLUSIONS
It is suggested that endogenous HO/CO system plays an important role in the process of IN induced by LPS, and inhibition of HO activity may decrease the formation of IN.
Ping
W
W
Pediatric Surgery, First Clinical Hospital, Beijing Medical University, Beijing 100034.
Qizi
Y
Y
Hesheng
O
O
Lijia
T
T
Jun
Y
Y
Chaoshu
T
T
D000818:Animals; D002248:Carbon Monoxide / Q000378:metabolism*; D002263:Carboxyhemoglobin / Q000378:metabolism; D003106:Colon / Q000378:metabolism*; D006152:Cyclic GMP / Q000097:blood / Q000378:metabolism; D003905:Deuteroporphyrins / Q000494:pharmacology*; D006419:Heme Oxygenase (Decyclizing) / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007443:Intussusception / Q000139:chemically induced / Q000378:metabolism*; D008070:Lipopolysaccharides; D008297:Male; D009130:Muscle, Smooth / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D003905:Deuteroporphyrins; D008070:Lipopolysaccharides; C060750:zinc deuteroporphyrin IX 2,4-bis(glycol); D002248:Carbon Monoxide; D002263:Carboxyhemoglobin; D006419:Heme Oxygenase (Decyclizing); D006152:Cyclic GMP