Comparison of bacterial enriched-broth culture, enzyme linked immunosorbent assay, and broth culture-polymerase chain reaction techniques for identifying asymptomatic infections with Salmonella in swine.
A polymerase chain reaction (PCR) assay was combined with a broth-culture enrichment system to detect Salmonella shed in feces from subclinically infected swine. The effectiveness of the broth culture-polymerase chain reaction (BC-PCR) assay to identify pigs shedding Salmonella in feces was compared with a microbiological culture and a commercial enzyme linked immunosorbent assay (ELISA) kit to detect Salmonella-specific serum antibody. A total of 67 pigs were tested by each of the 3 methodologies. Forty-one pigs tested positive for Salmonella by BC-PCR and ELISA identified 6 positives and 23 suspicious samples. It was shown that the BC-PCR assay is a rapid diagnostic tool for detecting of Salmonella shed by asymptomatic swine compared with current diagnostic technologies.
Sibley
Jennifer
J
Department of Veterinary Pathology, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan S7N 5B4.
Yue
Binbin
B
Huang
Fei
F
Harding
John
J
Kingdon
Jill
J
Chirino-Trejo
Manuel
M
Appleyard
Greg D
GD
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D003470:Culture Media; D004269:DNA, Bacterial / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D005243:Feces / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D011897:Random Allocation; D015203:Reproducibility of Results; D012475:Salmonella / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D012481:Salmonella Infections, Animal / Q000175:diagnosis* / Q000382:microbiology; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000382:microbiology
D000907:Antibodies, Bacterial; D003470:Culture Media; D004269:DNA, Bacterial