[Spectra studies on interaction of bovine serum albumin with acidic chrome blue K in acidic solution].
This paper investigated the interaction of acidic chrome blue K with bovine serum albumin (BSA). When BSA was added into acidic chrome blue K solution at pH 2.87 HCl-NaAc buffter, bathochromic effect and hypochromicity were observed. With the increase in BSA concentration, the absorption peak at 523 nm decreased. It was considered that the combination of BSA with acidic chrome blue K is due to static electricity forces. The interaction is in accord with model of phase distribution. It was discussed the effect of acidity, concentration of acidic chrome blue K, ion strength to apparent binding constant Kc, binding number n, Sandell constant. The reaction time, surfactant, work curve were studied.
Yu
Ying
Y
Department of Chemistry, South China Normal University, Guangzhou 510631, China.
Liao
Jian
J
Huang
Fa-de
FD
D000818:Animals; D001391:Azo Compounds / Q000737:chemistry*; D002417:Cattle; D004396:Coloring Agents / Q000737:chemistry; D004347:Drug Interactions; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet
D001391:Azo Compounds; D004396:Coloring Agents; D012996:Solutions; C039813:calcon; D012710:Serum Albumin, Bovine