[Experimental study of modified bio-derived material by pluronic F-127 in vitro].
OBJECTIVE
To investigate the effects of three-dimentional culture in bioderived material modified by Pluronic F-127 on the growth and function of rabbit periosteal osteoblast in vitro.


METHODS
Bio-derived materials were from fresh pig ribs and were modified by Pluronic F-127. Then rabbit periosteal osteoblasts were cultured in bio-derived materials(group A), in the modified bio-derived materials(group B) and on the plastic surfaces as a control (group C), respectively. During a 7-day period, the status of growth, cell viability and alkaline phosphatase(ALP) activity were measured.


RESULTS
Osteoblasts attached, elongated and grew well on the modified bio-derived materials. There were no significant difference in osteogenesis and ALP activity between group A and group B(P > 0.05). The osteogenesis and ALP activity in groups A, B were less than those in group C (P < 0.01).


CONCLUSIONS
Pluronic F-127 can be used for a carrier for bioactive factors to modify bio-derived material.
Ren
Yong-xin
YX
Key Laboratory of Transplant Engineering and Immunology of Ministry of Public Health, Institute of Reparative and Reconstructive Surgery, Laboratory of Tissue Engineering, West China Hospital, Sichuan University, Chengdu, Sichuan, P. R. China 610041.
Yang
Zhi-ming
ZM
Li
Xiu-qun
XQ
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D008422:Materials Testing; D010006:Osteoblasts / Q000187:drug effects* / Q000502:physiology; D020442:Poloxamer / Q000494:pharmacology*; D011817:Rabbits; D023822:Tissue Engineering
D001672:Biocompatible Materials; D020442:Poloxamer