[Synthesis and secretion of Bacillus intermedius proteinases in the late stages of sporulation].
In the late stages of sporulation, cells of Bacillus intermedius 3-19 secreted into the medium two proteinases, glutamyl endopeptidase and subtilisin, whose maximum activities were recorded in the 40th and 44th hours of growth, respectively. By estimating beta-galactosidase activity as a marker of cytoplasmic membrane integrity, it was revealed that the accumulation of these proteinases in the medium was a result of their secretion and not of lysis of the cell envelope. Concentrations of peptone and inorganic phosphate ensuring the maximum production of the enzymes were established. Ammonium ions were shown to inhibit the production of proteinases by the mechanism of repression by nitrogen metabolites.
Balaban
N P
NP
Kazan State University, ul. Kremlevskaya 18, Kazan, 420008 Russia. Nelly.Balaban@ksu.ru
Sharipova
M P
MP
Gabdrakhmanova
L A
LA
Mardanova
A M
AM
Tokmakova
Iu S
IuS
Sokolova
E A
EA
Rudenskaia
G N
GN
Leshchinskaia
I B
IB
D001407:Bacillus / Q000201:enzymology / Q000254:growth & development / Q000502:physiology*; D003470:Culture Media; D010461:Peptones; D010710:Phosphates; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D012697:Serine Endopeptidases / Q000032:analysis / Q000096:biosynthesis*; D013171:Spores, Bacterial / Q000201:enzymology; D020860:Subtilisin / Q000032:analysis / Q000096:biosynthesis*; D013997:Time Factors; D001616:beta-Galactosidase / Q000378:metabolism
D003470:Culture Media; D010461:Peptones; D010710:Phosphates; D000644:Quaternary Ammonium Compounds; D001616:beta-Galactosidase; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase; D020860:Subtilisin