Oocyte competence in repeat-breeder heifers: effects of an optimized ovum pick-up schedule on expression of oestrus, follicular development and fertility.
Repeat-breeder heifers (RBH) have been shown to present reproductive perturbations during spontaneous cyclicity, which affects oestrus and ovulation. Some of these disturbances (e.g. deviating hormone patterns) are also present during and after cycles of twice-weekly ovum pick-up (o.p.u.), performed according to an optimized schedule allowing normal oestrous cyclicity. In the present study, the effects of o.p.u. on oocyte competence in in vitro maturation (i.v.m.) and in vitro fertilization (i.v.f.) have been evaluated, as were the effects on expression of oestrus and fertility in five RBH (> or =4 artificial inseminations) and five virgin heifers (VH controls). In total, 269 RBH and 174 VH oocytes were scored for quality prior to i.v.m. and i.v.f. The number of follicles available for puncture was higher in RBH, but the oocyte recovery rate after o.p.u. was lower in RBH compared with VH controls and the recovered RBH oocytes were of lower quality, as judged by their appearance at retrieval. Confocal laser scanning and transmission electron microscopy of immature oocytes did not reveal any differences between RBH and VH control oocytes with respect to nuclear and mitochondrial status. However, after i.v.m., the cytoplasmic spatial reorganization of mitochondria and cortical granules was less advanced in RBH, which could contribute to the subfertility that defines the syndrome. Cleavage rates after i.v.f. were similar in RBH and VH controls. Subsequent to the o.p.u. period, in vivo fertility after controlled artificial insemination was comparable with field fertility rates in both RBH and VH.
Båge
Renée
R
Centre for Reproductive Biology in Uppsala (CRU), Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, PO Box 7039, SE-750 07 Uppsala, Sweden. renee.bage@og.slu.se
Petyim
Sudsaijai
S
Larsson
Birgitta
B
Hallap
Triin
T
Bergqvist
Ann-Sofi
AS
Gustafsson
Hans
H
Rodríguez-Martínez
Heriberto
H
D000818:Animals; D001947:Breeding; D002417:Cattle / Q000502:physiology*; D002467:Cell Nucleus / Q000648:ultrastructure; D002478:Cells, Cultured; D002970:Cleavage Stage, Ovum; D003593:Cytoplasm / Q000648:ultrastructure; D004971:Estrus; D005260:Female; D005298:Fertility; D005307:Fertilization in Vitro / Q000662:veterinary; D007315:Insemination, Artificial / Q000662:veterinary; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D008928:Mitochondria / Q000648:ultrastructure; D009865:Oocytes / Q000502:physiology* / Q000648:ultrastructure; D006080:Ovarian Follicle / Q000502:physiology*; D011374:Progesterone / Q000097:blood; D020858:Tissue and Organ Harvesting / Q000379:methods / Q000662:veterinary*
D011374:Progesterone