Expression of ICAM-1 and LFA-1 molecules in relation to renal allograft rejection in rats.
OBJECTIVE
The purpose of this study was to assess the renal graft expression of ICAM-1 (intercellular adhesion molecule-1) and LFA-1 (lymphocyte function-associated antigen-1) molecule with relation to graft rejection.


METHODS
Rat kidney transplantation was performed according to the procedure of Kamada with some modification. Experimental rats were divided into 5 groups. The survival time of recipient rats and function of grafts after renal transplantation were observed. The sections of renal graft were stained for monoclonal antibody ICAM-1 and LFA-1, and then quantification of ICAM-1 and LFA-1 expression was accomplished by computer image analysis.


RESULTS
ICAM-1 and LFA-1 increased significantly in the renal allograft rejection group as compared with the non-rejection groups (P<0.05).


CONCLUSIONS
Both biopsy of renal graft and monitoring of ICAM-1 and LFA-1 are useful tools in diagnosing and treating acute rejection.
Huang
X
X
Department of General Surgery, The First Affiliated Hospital, West China University of Medical Sciences, Chengdu 610041.
Shen
W
W
Li
Y
Y
Zhou
Z
Z
Tan
J
J
D000818:Animals; D006084:Graft Rejection / Q000378:metabolism* / Q000473:pathology; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism* / Q000473:pathology; D016030:Kidney Transplantation; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D014184:Transplantation, Homologous
D016169:Lymphocyte Function-Associated Antigen-1; D018799:Intercellular Adhesion Molecule-1