Chemical cross-linking and mass spectrometry for protein structural modeling.
The growth of gene and protein sequence information is currently so rapid that three-dimensional structural information is lacking for the overwhelming majority of known proteins. In this review, efforts towards rapid and sensitive methods for protein structural characterization are described, complementing existing technologies. Based on chemical cross-linking and offering the analytical speed and sensitivity of mass spectrometry these methodologies are thought to contribute valuable tools towards future high throughput protein structure elucidation.
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Jaap Willem
JW
Swammerdam Institute for Life Sciences (SILS), Mass Spectrometry group, University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV, Amsterdam, The Netherlands. jwback@science.uva.nl
de Jong
Luitzen
L
Muijsers
Anton O
AO
de Koster
Chris G
CG
D003432:Cross-Linking Reagents / Q000494:pharmacology*; D018360:Crystallography, X-Ray; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry / Q000379:methods*; D008854:Microscopy, Electron; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D012984:Software
D003432:Cross-Linking Reagents; D011506:Proteins