Accumulation and removal of heavy metal ions by insolubilized-DNA and its interaction.
DNA was immobilized onto a porous glass bead by a treatment with UV irradiation. The immobilized DNA was insoluble in water and used for accumulation of heavy metal ion. When DNA-immobilized glass bead was added into aqueous solution containing heavy metal ions, such as Hg2+, Cd2+, Pb2+, Zn2+, Cu2+ and Fe3+, the concentration of these metal ions in the solution was decreased. However, the concentration of Mg2+ in the solution was not affected by the addition of the DNA-immobilized glass bead. These results suggested that UV-irradiated DNA selectively accumulated heavy metal ions.
Yamada
M
M
Division of Bioscience, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo 060-0810, Japan.
Kato
K
K
Nomizu
M
M
Sakairi
N
N
Ohkawa
K
K
Yamamoto
H
H
Nishi
N
N
D000818:Animals; D001665:Binding Sites; D004247:DNA / Q000378:metabolism* / Q000528:radiation effects; D005898:Glass; D066298:In Vitro Techniques; D019216:Metals, Heavy / Q000302:isolation & purification* / Q000378:metabolism*; D012474:Salmon; D012995:Solubility; D014466:Ultraviolet Rays
D019216:Metals, Heavy; D004247:DNA