title: Viscoelastic deformation response of red blood cells under conditions of oscillating centrifugal field.
abstract: The red cell deformation under the conditions of oscillating centrifugal fields was studied. Experiments were carried out with a modified Cell-Elastometer operating in oscillating mode (0.02 to 0.30 Hz). Gravitational acceleration was sinusoidally modulated between 620 g and 2250 g. At low frequencies (below 0.08 Hz), native red cells followed the applied stress without delay. At 0.09 Hz and up, the cellular deformation was still periodical and included an additional perturbation due to intracellular movements. This perturbation was analysed and quantified. The influence of alterations on the erythrocyte membrane by diamide was analysed to verify the sensitivity of this method. On increasing the membrane stiffness with low concentrations of diamide, the response to oscillatory centrifugal stress was impaired characteristically in terms of amplitude deformation. Based on tangential and centrifugal accelerations, a physical model was developed that describes the basic observable changes on varying the oscillation frequency. From the data it can be concluded that viscoelastic properties of red cells can be analysed and quantified using oscillatory centrifugal accelerations. The described method can become a valid tool to differentiate between membrane alterations or intracellular viscous modifications.
authors.affiliation: Institute of Physiology, Medical Faculty, Technical University of Aachen, 52057 Aachen, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Farges
authors.forename: R
authors.initials: R
authors.lastname: Grebe
authors.forename: M
authors.initials: M
authors.lastname: Baumann
mesh_terms: D000054:Acceleration; D002478:Cells, Cultured; D002498:Centrifugation / Q000379:methods*; D003958:Diamide / Q000494:pharmacology; D004548:Elasticity; D004907:Erythrocyte Deformability / Q000187:drug effects / Q000502:physiology*; D004910:Erythrocyte Membrane / Q000187:drug effects; D006801:Humans; D008954:Models, Biological; D013314:Stress, Mechanical; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D014783:Viscosity
chemical_list: D013439:Sulfhydryl Reagents; D003958:Diamide