title: Quantification analysis of translation initiation signal in vertebrate mRNAs: effect of nucleotides at positions +4(-)+6 upon efficiency of translation initiation.
abstract: Concerning the translation initiation signal in vertebrate mRNAs, a consensus, sequence, (GCC)GCC(A or G) CCATGG, has been proposed, but actual initiation sequences differ from it in a greater or lesser degree. Kozak monitored selection by ribosomes of the first versus second ATG codons as a function of mutations introduced at positions +4, and +6 of the first ATG codon. Codons possessing G at +4 strongly enhanced selection of the first ATG codon. However, ATG codon recognition was unaffected by most mutations in positions and +6. These data were well understood by our quantification analysis.
authors.affiliation: Division of Chemistry, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Iida
authors.forename: D
authors.initials: D
authors.lastname: Kanagu
mesh_terms: D000818:Animals; D001483:Base Sequence; D018387:Codon, Initiator / Q000235:genetics; D009154:Mutation; D010442:Peptide Chain Initiation, Translational; D012333:RNA, Messenger / Q000235:genetics*; D012270:Ribosomes / Q000378:metabolism
chemical_list: D018387:Codon, Initiator; D012333:RNA, Messenger