The HY2 gene as an efficient marker for transposon excision in Arabidopsis.
Transposable elements can generate germinal and somatic mutations, and hence represent a powerful tool for the analysis of gene function. Transposons from maize have been adapted to mutagenise the genomes of diverse species. The efficiency of these systems partly relies on the ease with which germinal (i.e. germinally transmitted) or somatic excisions can be detected. Here we describe the use of HY2, a gene that codes for an enzyme involved in the biosynthesis of the phytochrome chromophore, to monitor the excision of a Ds gene-trap element in Arabidopsis thaliana. Taking advantage of the altered germination and de-etiolation behaviour of a Ds -tagged hy2 mutant, we have designed an efficient protocol for the recovery of germinal revertants, making HY2 the most precocious excision marker available, to the best of our knowledge. In addition, HY2 is also useful for generating visible sectors in photosynthetic tissues, thanks to the somatic instability of this mutable hy2 allele.
Léonard
B
B
Direction des Sciences du Vivant, Département d'Ecophysiologie Végétale et Microbiologie, Laboratoire de Biologie du Développement des Plantes (LBDP) Bât.178, CEA Cadarache, 13108, Saint Paul-lez-Durance, France.
Creff
A
A
Desnos
T
T
D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D017931:DNA Primers; D004251:DNA Transposable Elements / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics*; D017930:Genes, Reporter; D005819:Genetic Markers; D005966:Glucuronidase; D016254:Mutagenesis, Insertional / Q000235:genetics*; D009154:Mutation; D010455:Peptides; D010834:Phytochrome; D036226:Seedlings / Q000235:genetics* / Q000254:growth & development; D017385:Sequence Homology; D015533:Transcriptional Activation / Q000235:genetics*; D014170:Transformation, Genetic
D017931:DNA Primers; D004251:DNA Transposable Elements; D005819:Genetic Markers; D010455:Peptides; D010834:Phytochrome; D005966:Glucuronidase