[Inhibition effect of aspirin on the growth of gastric cancer and the mechanism there-in involved].
OBJECTIVE
To investigate the effect of aspirin on the growth of gastric cancer in vitro and the relevant mechanism.


METHODS
The effect of aspirin on proliferation of SGC-7901 cells was measured by 3H-thymidine incorporation into DNA; the cell cycle was analyzed by flow cytometry. COX-2 protein was examined in SGC-7901 cells by immunohistochemistry. The expression of c-fos and the AP-1 activity were detected by immunoblotting and EMSA respectively.


RESULTS
Aspirin decreased 3H-thymidine incorporation into SGC-7901 cells. The inhibition effect showed a good correlation with aspirin over a range of concentrations from 1 x 10(-1) mol/L to 1 x 10(-5) mol/L (r = 0.9, P < 0.01). Immunohistochemistry and immunoblotting indicated that aspirin effectively decreased COX-2 and c-fos expression in SGC-7901 cell. Aspirin could inhibit the AP-1 binding activation stimulated by fetal calf serum.


CONCLUSIONS
Aspirin is effective for inhibiting the growth of gastric cancer. The anti-neoplastic effect aspirin produces may involve the inhibition of COX-2 expression and AP-1 activity.
Wang
Chunhui
C
Laboratory of Peptides Related with Human Disease, Department of Gastroenterolgy, West China Hospital, Sichuan University, Chengdu 610041, China.
Tang
Chengwei
C
D000230:Adenocarcinoma / Q000473:pathology; D001241:Aspirin / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D051546:Cyclooxygenase 2; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D008565:Membrane Proteins; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D013274:Stomach Neoplasms / Q000473:pathology*; D018808:Transcription Factor AP-1 / Q000378:metabolism; D014407:Tumor Cells, Cultured
D007527:Isoenzymes; D008565:Membrane Proteins; D016760:Proto-Oncogene Proteins c-fos; D018808:Transcription Factor AP-1; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases; D001241:Aspirin