Antibody to soluble 1,3/1,6-beta-D-glucan, SCG in sera of naive DBA/2 mice.
A branched beta-glucan from Sparassis crispa (SCG) is a major 6-branched 1,3-beta-D-glucan showing antitumor activity. In the present study, we examined the anti-SCG antibody in naive mice by ELISA. Using SCG coated plate, sera of naive DBA/1 and DBA/2 mice contained significantly higher titers of antibody than other strains of mice. Anti-SCG Ab titers of each DBA/1 and DBA/2 mice were significantly varied. Using various polysaccharide-coated plate, sera of DBA/2 mice also reacted with a beta-glucan from Candida spp. (CSBG) having 1,3-beta and 1,6-beta-glucosidic linkages. The SCG specific immunoglobulin (Ig) M but G was detected in sera. The reactivity of sera to coated SCG was neutralized by adding soluble SCG and CSBG as competitor. These results suggested that DBA/1 and DBA/2 strains carry specific and unique immunological characteristics to branched 1,3-/1,6-beta-glucan.
Harada
Toshie
T
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy & Life Science, Tokyo, Japan.
Nagi Miura
Noriko
N
Adachi
Yoshiyuki
Y
Nakajima
Mitsuhiro
M
Yadomae
Toshiro
T
Ohno
Naohito
N
D000363:Agaricales / Q000276:immunology*; D000818:Animals; D000908:Antibodies, Fungal / Q000096:biosynthesis / Q000097:blood*; D000970:Antineoplastic Agents / Q000097:blood / Q000276:immunology; D005936:Glucans / Q000097:blood / Q000276:immunology*; D008297:Male; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D010936:Plant Extracts / Q000276:immunology; D012995:Solubility
D000908:Antibodies, Fungal; D000970:Antineoplastic Agents; D005936:Glucans; D010936:Plant Extracts; C033671:epiglucan