High resolution sequence-based DPB1 typing identified two novel DPB1 alleles, DPB1*9401 and DPB1*9501, from a Kenyan population.
Two novel DPB1 alleles, DPB1*9401 and DPB1*9501, were identified from a Kenyan population during sequence-based HLA-DPB1 typing. Molecular cloning and sequencing of multiple clones confirmed that one of the new DPB1 alleles is identical to DPB1*0402 at exon 2 except for a single nucleotide substitution (CGG -->TGG), changing codon 70 from Arg to Trp. The new allele has been named DPB1*9401. This is the first report of polymorphism at codon 70 of HLA-DPB1 alleles. New codon combinations have been identified in another novel DPB1 allele named DPB1*9501. The extensive diversity at DPB1 locus of this East African population is being revealed by high resolution sequence-based DPB1 typing.
Luo
M
M
Department of Medical Microbiology, University of Manitoba, Winnipeg, Canada.
Ramdahin
S
S
Iqbal
S
S
Pan
Y
Y
Jacobson
K
K
Narayansingh
M J
MJ
Schroeder
M
M
Brunham
R C
RC
Embree
J
J
Plummer
F A
FA
D000595:Amino Acid Sequence; D001483:Base Sequence; D006682:HLA-DP Antigens / Q000235:genetics*; D059868:HLA-DP beta-Chains; D006801:Humans; D007630:Kenya; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
D006682:HLA-DP Antigens; D059868:HLA-DP beta-Chains; C071170:HLA-DPB1 antigen