title: Effect of six candidate genes on early aging in a French population.
abstract: OBJECTIVE
  The objective of this study was to examine the association between an aging indicator previously defined from a nationwide population study, and lipids and apolipoproteins, angiotensin converting enzyme, paraoxonase activities, and six candidate genes related to the aging process.
  
  
  METHODS
  Two hundred and fifty-six healthy Caucasian men (69.8 +/- 4.0 years) were included in the study. Total cholesterol, triglycerides, HDL-cholesterol, lipoprotein(a), apolipoprotein A1, B and E concentrations, and the activities of paraoxonase, arylesterase, and angiotensin-converting enzymes were determined by standardized laboratory methods. A multiplex assay was used to genotype the studied polymorphisms: apolipoprotein E, lipoprotein lipase, paraoxonase, methylenetetrahydrofolate reductase, cystathionine beta-synthase and angiotensin-converting enzymes.
  
  
  RESULTS
  Paraoxonase polymorphism at codon 192 (Gln/Arg) was the only one significantly associated with the aging indicator, Gln homozygotes being more advanced in aging compared with Arg allele carriers. It was also observed that the aging indicator was positively correlated with serum concentrations of total cholesterol, triglycerides and apolipoprotein B, and negatively with the activities of basal and stimulated paraoxonase and arylesterase. Multiple regression analysis showed that triglycerides and basal paraoxonase activity explain 13.6% of the variance of the aging indicator.
  
  
  CONCLUSIONS
  Triglyceride concentration and paraoxonase gene and activities may contribute to the aging process. Taking into account the smallness of the sample size, and the poor level of significance due to the im-plication of paraoxonase polymorphism at codon 192, these results need to be verified in further studies on a greater number of subjects.
authors.affiliation: Laboratoire du Centre de Médecine Préventive and Unité INSERM 525, Vandoeuvre-lès-Nancy, France.
authors.forename: Yongjing
authors.initials: Y
authors.lastname: Xia
authors.forename: René
authors.initials: R
authors.lastname: Gueguen
authors.forename: Monique
authors.initials: M
authors.lastname: Vincent-Viry
authors.forename: Gérard
authors.initials: G
authors.lastname: Siest
authors.forename: Sophie
authors.initials: S
authors.lastname: Visvikis
mesh_terms: D000368:Aged; D000375:Aging / Q000235:genetics* / Q000502:physiology; D000483:Alleles; D001057:Apolipoproteins E / Q000097:blood / Q000235:genetics*; D043303:Aryldialkylphosphatase / Q000235:genetics* / Q000378:metabolism; D005602:France; D006801:Humans; D008297:Male; D011110:Polymorphism, Genetic; D020033:Protein Isoforms / Q000097:blood / Q000235:genetics; D014280:Triglycerides / Q000097:blood
chemical_list: D001057:Apolipoproteins E; D020033:Protein Isoforms; D014280:Triglycerides; D043303:Aryldialkylphosphatase