Tandem oligonucleotide synthesis using linker (First Base) phosphoramidites.
First Base linker phosphoramidites add the first nucleoside to underivatized solid-phase supports using a regular phosphoramidite coupling cycle. A cleavable 3'-O-ester linkage allows NH4OH treatment to rapidly generate products with only 3'-OH ends. Either single or multiple sequences linked in tandem can be prepared and no additional deprotection steps are required.
Pon
Richard T
RT
University of Calgary, Department of Biochemistry and Molecular Biology, Calgary, AB, Canada T2N 4N1.
Yu
Shuyuan
S
D000577:Amides / Q000737:chemistry*; D019075:Electrophoresis, Capillary; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D010756:Phosphoric Acids / Q000737:chemistry*
D000577:Amides; D009841:Oligonucleotides; D010756:Phosphoric Acids; C011067:phosphoramidic acid