Further investigation of the epitope recognized by the new monoclonal antibody 2C9.
OBJECTIVE
We established a new monoclonal antibody (2C9) that reacted with prostate tissue. The immunohistochemical reactivity of this antibody is similar to anti-prostate-specific membrane antigen (PSMA). Herein, we report the antigenic determinant of 2C9 antibody.


METHODS
The reactivity of the antibody was characterized by immunohistochemical staining and the antigen target was characterized by amino acid sequencing after immuno-affinity purification from an LNCaP cell lysate and cloning of a cDNA using a mammalian expression cDNA cloning system.


RESULTS
The amino acid and nucleotide sequences for the antigen molecule recognized with 2C9 monoclonal antibody demonstrated identity with PSMA.


CONCLUSIONS
The target molecule of the 2C9 monoclonal antibody is PSMA, pointing to future diagnostic and therapeutic applications.
Kato
Keitaro
K
Department of Urology, Aichi Medical University School of Medicine, Nagakute, Aichi, Japan.
Yoshikawa
Kazuhiro
K
Taki
Tomohiro
T
Shitara
Kenya
K
Nakamura
Kazuyasu
K
Hirota
Maiko
M
Hanai
Nobuo
N
Nakamura
Kogenta
K
Kokubo
Hiroto
H
Mitsui
Kenji
K
Yamada
Yoshiaki
Y
Honda
Nobuaki
N
Ueda
Ryuzo
R
Saga
Shinsuke
S
Fukatsu
Hidetoshi
H
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000235:genetics / Q000276:immunology*; D015152:Blotting, Northern; D045744:Cell Line, Tumor / Q000276:immunology / Q000378:metabolism; D002999:Clone Cells / Q000276:immunology; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D018604:Epitope Mapping / Q000379:methods; D000939:Epitopes / Q000032:analysis*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011467:Prostate / Q000737:chemistry / Q000276:immunology; D017430:Prostate-Specific Antigen / Q000032:analysis* / Q000737:chemistry; D011471:Prostatic Neoplasms / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D020539:Sequence Analysis, Protein
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D018076:DNA, Complementary; D000939:Epitopes; D017430:Prostate-Specific Antigen