title: [The variation of Caspase3 activity in tanshinone induced NB4 cells apoptosis].
abstract: OBJECTIVE
  To study the variation and role of Caspase3 activity in the process of Tanshinone (Tan II A) induced NB4 cells apoptosis.
  
  
  METHODS
  NB4 cell apoptosis induced by Tan II A was demonstrated by cell morphology, DNA content analysis and DNA fragmentation assay. Caspase3 activity was determined by spectrofluorometry, and its inhibitory assay was performed using N-acetyl-Asp-Glu-Val-Asp-aldehyde(AC-DEVD-CHO).
  
  
  RESULTS
  Tan II A could induce NB4 cell apoptosis accompanied with increase of caspase3 activity. The induction of NB4 cell apoptosis by use of Tan II A could be partially inhibited by AC-DEVD-CHO.
  
  
  CONCLUSIONS
  The induction of NB4 cell apoptosis by Tan II A could be fulfilled by activating Caspase3.
authors.affiliation: Department of Hematology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Xiaogong
authors.initials: X
authors.lastname: Liang
authors.forename: Yiming
authors.initials: Y
authors.lastname: Yang
authors.forename: Chengqi
authors.initials: C
authors.lastname: Deng
authors.forename: Wentong
authors.initials: W
authors.lastname: Meng
authors.forename: Ting
authors.initials: T
authors.lastname: Liu
authors.forename: Yongqian
authors.initials: Y
authors.lastname: Jia
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Cheng
authors.forename: Li
authors.initials: L
authors.lastname: Li
mesh_terms: D045784:Abietanes; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism*; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000188:drug therapy* / Q000473:pathology; D010616:Phenanthrenes / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D045784:Abietanes; D000972:Antineoplastic Agents, Phytogenic; D010616:Phenanthrenes; C021751:tanshinone; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases