Multitasking with molecular dynamics Typhoon: quantifying nucleic acids and autoradiographs.
With increased sensitivity and specificity, fluorescent assays are rapidly becoming the method of choice for nucleic acid quantification. The utility of the Typhoon scanner has now been extended to accurately measure low levels of DNA and RNA (5 ng ml(-1)) with PicoGreen and RiboGreen dyes. In addition, with a few simple modifications, autoradiographic film images can be scanned and quantified with the Typhoon series of scanners.
Goodrich
Robert J
RJ
Department of Obstetrics and Gynecology, Wayne State University, Detroit, MI 48201, USA.
Ostermeier
G Charles
GC
Krawetz
Stephen A
SA
D001345:Autoradiography / Q000295:instrumentation* / Q000379:methods; D004247:DNA / Q000032:analysis*; D019544:Equipment Failure Analysis; D005456:Fluorescent Dyes; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D017403:In Situ Hybridization / Q000295:instrumentation / Q000379:methods; D012313:RNA / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D005456:Fluorescent Dyes; D012313:RNA; D004247:DNA