title: Release and regeneration of protoplasts from the fungus Trichothecium roseum.
abstract: A protocol for isolating and regenerating protoplasts from Trichothecium roseum has been described. Protoplasts from T. roseum were isolated using (i) a lytic enzyme combination composed of Novozym 234, chitinase, cellulase, and pectinase at a 5-mg/mL concentration and (ii) 0.6 M KCl as an osmotic stabilizer. A maximum number of 28 x 10(4) protoplasts/mL were obtained at pH 5.5. Experiments on the regeneration and reversion of protoplasts revealed a maximum regeneration (60.8%) in complete medium (potato dextrose--yeast extract agar) amended with 0.6 M KCl. The regenerated protoplasts were similar to the original parent strain in morphology, pigmentation, growth, and sporulation.
authors.affiliation: Center for Advanced Studies in Botany, University of Madras, Chennai, India.
authors.forename: N
authors.initials: N
authors.lastname: Balasubramanian
authors.forename: G Annie
authors.initials: GA
authors.lastname: Juliet
authors.forename: P
authors.initials: P
authors.lastname: Srikalaivani
authors.forename: D
authors.initials: D
authors.lastname: Lalithakumari
mesh_terms: D002480:Cellulase / Q000378:metabolism; D002688:Chitinases / Q000378:metabolism; D003470:Culture Media; D004798:Enzymes / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D003904:Mitosporic Fungi / Q000502:physiology* / Q000648:ultrastructure; D025282:Mycelium / Q000502:physiology; D009172:Mycology / Q000379:methods*; D009994:Osmolar Concentration; D011096:Polygalacturonase / Q000378:metabolism; D011523:Protoplasts / Q000502:physiology*; D013696:Temperature
chemical_list: D003470:Culture Media; D004798:Enzymes; C045154:novozym 234; D002688:Chitinases; D011096:Polygalacturonase; D002480:Cellulase