Photoreaction of caged ATP studied by the time-resolved transient grating method.
The photoreaction of caged ATP, P3-[1-(2-nitrophenyl)ethyl]adenosine-5'-triphosphate, has been investigated using the time-resolved transient grating (TG) method. We found that a feature of the TG signal time profile depends sensitively on the grating wavenumber (q) after the photoexcitation of caged ATP. This q-dependent feature of the TG signal was interpreted based on a model where the ATP release rate is comparable to the molecular diffusion process. We found that the TG signals at various q can be consistently analyzed based on this model and the ATP release rate determined. The enthalpy and volume changes of the reaction have been determined by quantitative measurement of the grating and photoacoustic signals.
Choi
Jungkwon
J
Department of Chemistry, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan.
Terazima
Masahide
M
D000162:Acoustics; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000737:chemistry*; D004058:Diffusion; D007700:Kinetics; D008956:Models, Chemical; D010777:Photochemistry; D013816:Thermodynamics; D013997:Time Factors
C029273:P(3)-1-(2-nitro)phenylethyladenosine 5'-triphosphate; D000255:Adenosine Triphosphate