[Identification of Taenia saginata by mtCO I in four areas of Yunnan and Guizhou provinces].
OBJECTIVE
To identify the types of Taenia saginata isolated from Dali of Yunnan Province, and from Duyun and Congjiang of Guizhou Province.


METHODS
Genomic DNA was isolated, and the mitochondrial cytochrome C oxidase subunit I (mtCO I) genes were amplified by polymerase chain reaction(PCR) and analyzed by PHYLIP software package.


RESULTS
The mtCO I gene sequences of Lanping sample were identical to that of T. saginata asiatica known in Taiwan, and the samples obtained from Dali and Duyun showed the same mtCO I gene sequences, while the sample from Congjiang had the same mtCO I gene sequences as T. saginata. The homology between these two groups of gene sequences was 97.44%, while the homology of amino acid sequences reached to 99.16%. The constructed phylogenetic tree revealed that the relationship between T. saginata asiatica and T. saginata is closer, both are distant relative to T. solium and other species of cestodes.


CONCLUSIONS
The Taenia prevalent in Lanping, Dali and Duyun is identified as T. saginata asiatica, while that isolated in Congjiang is the typical T. saginata.
Wang
Zheng-rong
ZR
Department of Laboratory Medicine, Guiyang Medical College, Guiyang 550004.
Bao
Huai-en
HE
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002681:China; D003576:Electron Transport Complex IV / Q000235:genetics*; D008928:Mitochondria / Q000201:enzymology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013621:Taenia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
D003576:Electron Transport Complex IV