Micronuclei to detect in vivo chemotherapy damage in a p53 mutated solid tumour.
Apoptosis induction and micronuclei formation were compared following cytotoxic treatments in two rat glioma differing in p53 integrity. In vitro, micronuclei emergence but not apoptosis was linked to the p53 mutated status. In vivo, micronuclei assays were more sensitive to evaluate DNA damage induced by chemotherapy in a p53-mutated solid tumour.
Driessens
G
G
Interdisciplinary Research Institute (IRIBHM), Faculty of Medicine, Université Libre de Bruxelles, 1070 Brussels, Belgium.
Harsan
L
L
Robaye
B
B
Waroquier
D
D
Browaeys
P
P
Giannakopoulos
X
X
Velu
T
T
Bruyns
C
C
D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D000970:Antineoplastic Agents / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects; D001932:Brain Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000532:radiotherapy; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002945:Cisplatin / Q000627:therapeutic use; D004249:DNA Damage / Q000187:drug effects; D004273:DNA, Neoplasm / Q000187:drug effects; D005910:Glioma / Q000188:drug therapy / Q000235:genetics* / Q000532:radiotherapy; D020287:In Situ Nick-End Labeling; D048629:Micronuclei, Chromosome-Defective / Q000235:genetics*; D015162:Micronucleus Tests; D051381:Rats; D011916:Rats, Inbred F344; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
D017304:Annexin A5; D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D016159:Tumor Suppressor Protein p53; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases; D002945:Cisplatin