Redox-driven proton pumping by heme-copper oxidases.
One of the key problems of molecular bioenergetics is the understanding of the function of redox-driven proton pumps on a molecular level. One such class of proton pumps are the heme-copper oxidases. These enzymes are integral membrane proteins in which proton translocation across the membrane is driven by electron transfer from a low-potential donor, such as, e.g. cytochrome c, to a high-potential acceptor, O(2). Proton pumping is associated with distinct exergonic reaction steps that involve gradual reduction of oxygen to water. During the process of O(2) reduction, unprotonated high pK(a) proton acceptors are created at the catalytic site. Initially, these proton acceptors become protonated as a result of intramolecular proton transfer from a residue(s) located in the membrane-spanning part of the enzyme, but removed from the catalytic site. This residue is then reprotonated from the bulk solution. In cytochrome c oxidase from Rhodobacter sphaeroides, the proton is initially transferred from a glutamate, E(I-286), which has an apparent pK(a) of 9.4. According to a recently published structure of the enzyme, the deprotonation of E(I-286) is likely to result in minor structural changes that propagate to protonatable groups on the proton output (positive) side of the protein. We propose that in this way, the free energy available from the O(2) reduction is conserved during the proton transfer. On the basis of the observation of these structural changes, a possible proton-pumping model is presented in this paper. Initially, the structural changes associated with deprotonation of E(I-286) result in the transfer of a proton to an acceptor for pumped protons from the input (negative) side of the membrane. After reprotonation of E(I-286) this acceptor releases a proton to the output side of the membrane.
Brzezinski
Peter
P
Department of Biochemistry and Biophysics, The Arrhenius Laboratories for Natural Sciences, Stockholm University, Svante Arrhenius vag 12, SE-106 91, Stockholm, Sweden. peterb@dbb.su.se
Larsson
Gisela
G
D003300:Copper / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000502:physiology; D006418:Heme / Q000378:metabolism*; D008954:Models, Biological; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000502:physiology*; D017494:Proton Pumps / Q000502:physiology*; D012242:Rhodobacter sphaeroides / Q000201:enzymology
D017494:Proton Pumps; D006418:Heme; D003300:Copper; D010088:Oxidoreductases; D003576:Electron Transport Complex IV