[Transformation of specific antibodies to polyreactive immunoglobulins and their binding to blood vessel walls].
It was shown that 30-50% ethanol or 40-70% dimetilsulfoxide could efficiently induce in vitro transformation of specific monoclonal antibodies (mAbs) into non-specific polyreactive immunoglobulins (PRIG). Intravenous injection 0.4 ml of FeSO4-EDTA mixture (60 and 30 mkM respectively) could induce increase of PRIG reactivity in the blood-stream. Intramuscle injection of either 0.1 ml of 40% ethanol, or 0.1 ml of FeSO4-EDTA mixture into muscle of hind limb of C57B1 mice leads to the substantial binding of circulated immunoglobulins to the blood vessels of the muscle. The similar effect could also be induced by ischemia/reperfusion of mice hind limb. In the case of intravenous injection of specific to ovalbumin biotinilated mAbs, the subsequent intramuscle injection of 0.1 ml of 40% ethanol induces apparent transformation of these mAbs into PRIG and their binding to the blood vessels. Intramuscle injection of 0.1 ml of FeSO4-EDTA mixture induces less than ethanol though noticeable effect. The obtained data have shown that cord-blood circulating specific antibodies could be transformed into PRIG at some conditions in vivo. If so, this process might play an important role in the organism defence against infections but could, probably, facilitate the development of atherosclerosis, cardiac infarct, cerebral stroke or tumors.
Bobrovnik
S A
SA
Palladin Institute of Biochemistry, NAS of Ukraine, Kyiv. sab@biochem.kiev.ua
D000818:Animals; D000911:Antibodies, Monoclonal / Q000187:drug effects* / Q000378:metabolism*; D000918:Antibody Specificity / Q000187:drug effects; D000937:Antigen-Antibody Reactions; D001808:Blood Vessels / Q000187:drug effects / Q000378:metabolism*; D004121:Dimethyl Sulfoxide / Q000008:administration & dosage / Q000494:pharmacology; D004492:Edetic Acid / Q000008:administration & dosage / Q000378:metabolism; D000431:Ethanol / Q000008:administration & dosage / Q000494:pharmacology; D007136:Immunoglobulins / Q000097:blood / Q000276:immunology*; D066298:In Vitro Techniques; D007275:Injections, Intravenous; D051379:Mice; D008810:Mice, Inbred C57BL; D018482:Muscle, Skeletal / Q000098:blood supply / Q000187:drug effects / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects
D000911:Antibodies, Monoclonal; D007136:Immunoglobulins; D000431:Ethanol; D004492:Edetic Acid; D004121:Dimethyl Sulfoxide