Ribosomal protein P0 from Aedes albopictus mosquito cells: cDNA cloning and analysis of expression.
Ribosomal protein P0 is one of the highly conserved phosphorylated proteins in the large subunit of eukaryotic ribosomes. P0 has been shown in Drosophila to be a multifunctional protein that associates with elongation factor eEF2 to facilitate translation and also plays a role in DNA repair. In this paper we describe the cloning and characterization of the full-length cDNA encoding P0 from the mosquito, Aedes albopictus. In vitro translation showed that the cDNA encoded a 34kDa protein that corresponded in size to a phosphorylated protein from the large ribosomal subunit after in vivo labeling with [32P]orthophosphate. When cells were exposed to various stresses, expression of P0 appeared to differ from that of rpL34. Preliminary RNAi experiments suggested that downregulation of P0 is correlated with apoptosis in C7-10 mosquito cells.
Jayachandran
Gitanjali
G
Department of Entomology, University of Minnesota, 1980 Folwell Avenue, St. Paul, MN 55108, USA. jayac001@umn.edu
Fallon
Ann M
AM
D000330:Aedes / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics*; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000235:genetics*; D012269:Ribosomal Proteins / Q000235:genetics*
D018076:DNA, Complementary; D010750:Phosphoproteins; D012269:Ribosomal Proteins; C054675:ribosomal protein P0