title: Differentiation of bone marrow stromal stem cells into cardiomyocyte-like cells in different mammalian species.
abstract: We describe the possibility of obtaining cardiomyocyte-like cell cultures from rat, guinea pig, and human bone marrow stromal stem cells. The content of troponin I-positive cells attains 35-45% of the total number of cells in the cultures and persists at this level for up to 4 months under differentiation conditions. Spontaneous contractions of cardiomyocyte-like cells were observed after the formation of cell monolayer under differentiation conditions.
authors.affiliation: Institute of Transplantology and Artificial Organs, Ministry of Health of Russian Federation, Moscow.
authors.forename: V I
authors.initials: VI
authors.lastname: Shumakov
authors.forename: N A
authors.initials: NA
authors.lastname: Onishchenko
authors.forename: M E
authors.initials: ME
authors.lastname: Krasheninnikov
authors.forename: V A
authors.initials: VA
authors.lastname: Zaidenov
authors.forename: I V
authors.initials: IV
authors.lastname: Potapov
authors.forename: L V
authors.initials: LV
authors.lastname: Bashkina
authors.forename: A V
authors.initials: AV
authors.lastname: Bersenev
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology*; D048429:Cell Size; D002478:Cells, Cultured; D003470:Culture Media; D006168:Guinea Pigs; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000502:physiology*; D006801:Humans; D009200:Myocardial Contraction; D032383:Myocytes, Cardiac / Q000166:cytology / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology*; D019210:Troponin I / Q000378:metabolism
chemical_list: D003470:Culture Media; D019210:Troponin I