Fast LC/MS in the analysis of small molecules.
Liquid chromatography-mass spectrometry (LC/MS) has become one of the most widely used analytical techniques in both qualitative and quantitative analysis of small molecules. Recently, with the increasing demand for ever-higher sample throughput, the use of faster chromatographic separations has become popular, along with other LC/MS methods that decrease analytical cycle-time. The burgeoning use of LC/MS has meant that the primary expertise of many practitioners today is not in the field of LC/MS, which has been facilitated by the ease-of-use of modern LC/MS systems. An examination of the current state of the literature, relating to "fast LC/MS", should serve well to those new to LC/MS, and should help them in the development of fast LC/MS methods that are effective in terms of both the chromatography and the utilization of the mass spectrometer. This review paper focuses on fast LC/MS analyses of small molecules that have been reported in peer-reviewed publications.
Tiller
Philip R
PR
Drug Metabolism, Merck Research Laboratories, West Point, PA 19486, USA, philip_tiller@merck.com
Romanyshyn
Leslie A
LA
Neue
Uwe D
UD
D000818:Animals; D002623:Chemistry Techniques, Analytical / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008970:Molecular Weight; D004364:Pharmaceutical Preparations / Q000097:blood / Q000737:chemistry / Q000652:urine
D004364:Pharmaceutical Preparations