Avian Purkinje neuronal cultures: extrinsic control of morphology by cell type and glutamate.
An in vitro coculture system is described to study the avian Purkinje neuron and the interactions occurring with astrocytes and granule cells during development in the cerebellum. Astrocytes initially and granule cells later regulate Purkinje neuron morphology. The coculture system presented here provides an excellent system for investigating the morphological, immunocytochemical, and electrophysiological differentiation of Purkinje neurons under controlled conditions and for studying cell-cell interactions and extrinsic factors, e.g., glutamate in normal and neuropathological conditions.
Jeffrey
Peter L
PL
Developmental Neurobiology Group, Children's Medical Research Institute, Westmead, New South Wales 2145, Australia.
Balcar
Vladimir J
VJ
Tolhurst
Ornella
O
Weinberger
Ron P
RP
Meany
Jenny A
JA
D000818:Animals; D001253:Astrocytes / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D002450:Cell Communication / Q000187:drug effects / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D002642:Chick Embryo; D018920:Coculture Techniques / Q000295:instrumentation / Q000379:methods; D027342:Excitatory Amino Acid Transporter 2 / Q000037:antagonists & inhibitors / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism / Q000494:pharmacology*; D011689:Purkinje Cells / Q000166:cytology* / Q000187:drug effects* / Q000502:physiology
D027342:Excitatory Amino Acid Transporter 2; D018698:Glutamic Acid