A gene transfer system for the glycopeptide producer Nonomuraea sp. ATCC39727.
The filamentous actinomycete Nonomuraea sp. ATCC39727 produces the industrially important glycopeptide antibiotic A40926. We developed a gene transfer system based on intergeneric conjugation from Escherichia coli. Analysis of the ex-conjugants revealed that the incoming plasmid pSET152 had integrated at two sites in the Nonomuraea genome. One of these was characterized and found to be highly related to other PhiC31 attB sites described in Streptomyces spp., including the core TTS sequence, where crossover occurs. Surprisingly, pSET152 was also found in episomic form in the Nonomuraea ex-conjugants.
Stinchi
Sofia
S
Vicuron Pharmaceuticals, Via Lepetit 34, 21040 Gerenzano (VA), Italy.
Azimonti
Sara
S
Donadio
Stefano
S
Sosio
Margherita
M
D000192:Actinomycetales / Q000235:genetics* / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001287:Attachment Sites, Microbiological / Q000235:genetics; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics*; D001435:Bacteriophages / Q000235:genetics; D001483:Base Sequence; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D018014:Gene Transfer Techniques; D005798:Genes, Bacterial; D006020:Glycopeptides; D011995:Recombination, Genetic; D017334:Teicoplanin / Q000031:analogs & derivatives
C054981:A 40926; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D006020:Glycopeptides; D017334:Teicoplanin