Expression of CS-1 fibronectin precedes monocyte chemoattractant protein-1 production during elicitation of allergic contact dermatitis.
BACKGROUND
Leucocyte migration within inflammatory skin compartments in allergic contact dermatitis (ACD) is the result of a sophisticated multi-step event where multiple molecules are involved.


OBJECTIVE
Since non-antigen-specific mechanisms have been described as an early participant in elicitation of ACD, we investigated the kinetics of the expression of monocyte chemoattractant protein-1 (MCP-1/CCL2) and the type of infiltrating cells. We compared the time course production of MCP-1/CCL2 with connecting segment-1 (CS-1) fibronectin and thymus and activation-regulated chemokine (TARC/ CCL17) expression.


METHODS
Biopsies from 10 individuals challenged in their back with the antigen responsible for their contact dermatitis and an irrelevant antigen were taken at different times and histology, immunohistochemistry for CS-1 fibronectin, TARC/CCL17, CD3, CD68, CXCR3, CCR4 and in situ hybridization for MCP-1/CCL2 were performed.


RESULTS
At positive antigen stimulated sites expression of MCP-1/CCL2 by basal keratinocytes and isolated cells in dermis started at 10 h. CS-1 fibronectin and TARC/CCL17 expression by blood endothelial cells was found at 2 and 10 h, respectively. This was followed by dermal accumulation of mononuclear cells with a significant increase of CD3+ and CD68+cells. At 48 h, approximately 58% of infiltrating cells were CXCR3+, and 35% CCR4+.


CONCLUSIONS
We showed evidence of the fact that CS-1 fibronectin expression precedes the production of MCP-1/CCL2 and TARC/CCL17 in the skin of patients with ACD, suggesting that these molecules participate in the early complex process of migrating mononuclear cells during elicitation of ACD.
Martín
A P
AP
Inmunología, Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
Gagliardi
J
J
Baena-Cagnani
C E
CE
Eberhard
Y
Y
Uguccioni
M
M
Gallino
N
N
Mariani
A L
AL
Serra
H M
HM
D000328:Adult; D000368:Aged; D001706:Biopsy; D002352:Carrier Proteins / Q000096:biosynthesis*; D054414:Chemokine CCL17; D018932:Chemokine CCL2 / Q000096:biosynthesis* / Q000235:genetics; D019742:Chemokines, CC / Q000096:biosynthesis; D017449:Dermatitis, Allergic Contact / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D005260:Female; D015870:Gene Expression; D006801:Humans; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D009842:Oligopeptides / Q000096:biosynthesis*; D010328:Patch Tests / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics; D012867:Skin / Q000276:immunology / Q000378:metabolism
C106230:CCL17 protein, human; D002352:Carrier Proteins; D054414:Chemokine CCL17; D018932:Chemokine CCL2; D019742:Chemokines, CC; D009842:Oligopeptides; D012333:RNA, Messenger; C094697:glutamyl-isoleucyl-leucyl-aspartyl-valine