title: [Immunofluorescence identification of limbal stem cells in a culture of donor corneas].
abstract: The purpose of this study was to identify limbal stem cells in the culture of human donor cornea. After standard cultivation procedure we used monoclonal antibody against alpha-enolase for identification of stem cells. This antibody serves as a probe for the identification of biochemical marker of stem cells. Using immunofluorescent microscopy we identified in specimens alpha-enolase positive cells which in vitro but not in situ showed also some morphologic differences from other cellular elements of the culture.
authors.affiliation: Ocná klinika LF UPJS, Kosice.
authors.forename: I
authors.initials: I
authors.lastname: Kozák
authors.forename: J
authors.initials: J
authors.lastname: Rosocha
authors.forename: H
authors.initials: H
authors.lastname: Hrbková
authors.forename: T
authors.initials: T
authors.lastname: Juhás
mesh_terms: D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D016039:Corneal Transplantation; D005455:Fluorescent Antibody Technique; D006801:Humans; D016850:Limbus Corneae / Q000166:cytology*; D010751:Phosphopyruvate Hydratase / Q000032:analysis* / Q000276:immunology; D013234:Stem Cells / Q000737:chemistry / Q000166:cytology*
chemical_list: D000911:Antibodies, Monoclonal; D010751:Phosphopyruvate Hydratase