The conformation of myosin head domains in rigor muscle determined by X-ray interference.
In the absence of adenosine triphosphate, the head domains of myosin cross-bridges in muscle bind to actin filaments in a rigor conformation that is expected to mimic that following the working stroke during active contraction. We used x-ray interference between the two head arrays in opposite halves of each myosin filament to determine the rigor head conformation in single fibers from frog skeletal muscle. During isometric contraction (force T(0)), the interference effect splits the M3 x-ray reflection from the axial repeat of the heads into two peaks with relative intensity (higher angle/lower angle peak) 0.76. In demembranated fibers in rigor at low force (<0.05 T(0)), the relative intensity was 4.0, showing that the center of mass of the heads had moved 4.5 nm closer to the midpoint of the myosin filament. When rigor fibers were stretched, increasing the force to 0.55 T(0), the heads' center of mass moved back by 1.1-1.6 nm. These motions can be explained by tilting of the light chain domain of the head so that the mean angle between the Cys(707)-Lys(843) vector and the filament axis increases by approximately 36 degrees between isometric contraction and low-force rigor, and decreases by 7-10 degrees when the rigor fiber is stretched to 0.55 T(0).
Reconditi
M
M
Laboratorio di Fisiologia, Dipartimento di Biologia Animale e Genetica, University of Florence, Florence, Italy.
Koubassova
N
N
Linari
M
M
Dobbie
I
I
Narayanan
T
T
Diat
O
O
Piazzesi
G
G
Lombardi
V
V
Irving
M
M
D000199:Actins / Q000737:chemistry / Q000648:ultrastructure; D000818:Animals; D032701:Biomimetics / Q000379:methods*; D018360:Crystallography, X-Ray / Q000379:methods*; D004548:Elasticity; D007537:Isometric Contraction; D008954:Models, Biological; D008958:Models, Molecular; D020409:Molecular Motor Proteins / Q000737:chemistry* / Q000648:ultrastructure; D009068:Movement; D018482:Muscle, Skeletal / Q000503:physiopathology* / Q000648:ultrastructure; D009218:Myosins / Q000737:chemistry* / Q000648:ultrastructure; D011487:Protein Conformation; D012298:Rigor Mortis / Q000473:pathology / Q000503:physiopathology*; D013314:Stress, Mechanical; D013329:Structure-Activity Relationship
D000199:Actins; D020409:Molecular Motor Proteins; D009218:Myosins