title: Modified DNA aptamers against sweet agent aspartame.
abstract: We obtained a modified DNA aptamer against sweetener, aspartame, by in vitro selection method. The modified DNA was prepared from dATP, dGTP, dCTP and a modified dTTP bearing a terminal amino group at C-5 position in place of thymidine by PCR using a hyper thermophilic DNA polymerase, KOD Dash DNA polymerase. The synthetic 102-mer DNA with a 60-mer random region was used as an initial template for the PCR. The PCR-amplified modified DNA library was applied to an aspartame-agarose column, and then the bound modified DNA was eluted from the column for the affinity chromatography selection. Repeating the procedure, we selected the modified DNA aptamer against aspartame.
authors.affiliation: Department of Applied Chemistry, Faculty of Engineering, Gunma University, Kiryu, Gunma 376-8515, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Saitoh
authors.forename: Akiko
authors.initials: A
authors.lastname: Nakamura
authors.forename: Masayasu
authors.initials: M
authors.lastname: Kuwahara
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ozaki
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Sawai
mesh_terms: D001218:Aspartame / Q000737:chemistry*; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D017931:DNA Primers; D016133:Polymerase Chain Reaction; D013549:Sweetening Agents / Q000737:chemistry*
chemical_list: D017931:DNA Primers; D013549:Sweetening Agents; D004247:DNA; D001218:Aspartame