title: Enhanced formaldehyde detoxification by overexpression of glutathione-dependent formaldehyde dehydrogenase from Arabidopsis.
abstract: The ADH2 gene codes for the Arabidopsis glutathione-dependent formaldehyde dehydrogenase (FALDH), an enzyme involved in formaldehyde metabolism in eukaryotes. In the present work, we have investigated the potential role of FALDH in detoxification of exogenous formaldehyde. We have generated a yeast (Saccharomyces cerevisiae) mutant strain (sfa1Delta) by in vivo deletion of the SFA1 gene that codes for the endogenous FALDH. Overexpression of Arabidopsis FALDH in this mutant confers high resistance to formaldehyde added exogenously, which demonstrates the functional conservation of the enzyme through evolution and supports its essential role in formaldehyde metabolism. To investigate the role of the enzyme in plants, we have generated Arabidopsis transgenic lines with modified levels of FALDH. Plants overexpressing the enzyme show a 25% increase in their efficiency to take up exogenous formaldehyde, whereas plants with reduced levels of FALDH (due to either a cosuppression phenotype or to the expression of an antisense construct) show a marked slower rate and reduced ability for formaldehyde detoxification as compared with the wild-type Arabidopsis. These results show that the capacity to take up and detoxify high concentrations of formaldehyde is proportionally related to the FALDH activity in the plant, revealing the essential role of this enzyme in formaldehyde detoxification.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad Autónoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
authors.forename: Hakima
authors.initials: H
authors.lastname: Achkor
authors.forename: Maykelis
authors.initials: M
authors.lastname: Díaz
authors.forename: M Rosario
authors.initials: MR
authors.lastname: Fernández
authors.forename: Josep Antoni
authors.initials: JA
authors.lastname: Biosca
authors.forename: Xavier
authors.initials: X
authors.lastname: Parés
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Martínez
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D005557:Formaldehyde / Q000378:metabolism*; D015870:Gene Expression; D005978:Glutathione / Q000378:metabolism*; D030821:Plants, Genetically Modified; D015003:Yeasts / Q000235:genetics / Q000254:growth & development / Q000378:metabolism
chemical_list: D005557:Formaldehyde; D000445:Aldehyde Oxidoreductases; C087006:formaldehyde dehydrogenase, glutathione-independent; D005978:Glutathione