Effect of kanglemycin C on lymphokin a production and gene expression of mouse splenocyte and macrophage.
OBJECTIVE
To study the effect of kanglemycin C (KC) on production and gene transcription of lymphokins.


METHODS
Cell proliferation and lymphokin activities were quantified with MTT colorimetry and ELISA, and gene transcriptions of lymphokins semi-quantified with RT-PCR.


RESULTS
Suppression of KC on proliferation of enriched T-and B-cell respectively mediated by Con A and LPS was declined by addition of exogenous IL-1, IL-2, and IL-6. KC 80 nmol/L markedly inhibited IL-2 and IL-6 production and mRNA transcription of incubated mouse splenocytes induced by Con A. Additionally, KC had some suppression on IL-1beta and IL-6 productions of peritoneal macrophage stimulated by LPS (5microg/mL), whereas cyclosporine (CS) had not.


CONCLUSIONS
Immunosuppression of KC came true partially through the decrease of IL-1beta, -2 and -6 productions, especially of IL-2. However, CS's immunosuppression was mainly through the decrease of IL-2 production.
Li
J
J
Department of Pharmacology, Beijing Medical University, Beijing 100083.
Tan
H
H
Lin
Z
Z
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology; D002455:Cell Division / Q000187:drug effects; D016572:Cyclosporine / Q000494:pharmacology; D005260:Female; D007375:Interleukin-1 / Q000096:biosynthesis / Q000235:genetics; D007376:Interleukin-2 / Q000096:biosynthesis / Q000235:genetics; D015850:Interleukin-6 / Q000096:biosynthesis / Q000235:genetics; D008222:Lymphokines / Q000096:biosynthesis* / Q000235:genetics; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000235:genetics; D012294:Rifamycins / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology / Q000378:metabolism*; D013601:T-Lymphocytes / Q000166:cytology
D007375:Interleukin-1; D007376:Interleukin-2; D015850:Interleukin-6; D008222:Lymphokines; D012333:RNA, Messenger; D012294:Rifamycins; C400787:kanglemycin C; D016572:Cyclosporine