Osteoblastic cell response to thin film of poorly crystalline calcium phosphate apatite formed at low temperatures.
The response of osteoblastic cells to a thin film of poorly crystalline calcium phosphate apatite crystals (PCA) was examined in vitro. The PCA thin film was prepared on polystyrene culture dishes using highly metastable calcium phosphate ion solution at low temperatures. The PCA thin film was formed through fusion and transformation of granular calcium phosphate particles, which had initially formed on the surface, into a film of calcium phosphate apatite crystal. The PCA thin film was used for cell culture without additional surface treatment. The osteoblastic cell behaviors including adhesion, proliferation, expression of the marker genes, and calcified matrix formation were examined on the PCA thin film using primary osteoblasts or MC3T3-E1 cells. The cells were well attached and had spread in a slender shape over the PCA thin film. The extent of cell proliferation on the PCA thin film is as much as on the plain dishes. In addition, a much larger number of calcified nodules had formed on the PCA thin film than on the plain dish. The expression of the marker genes such as alkaline phosphatase, osteocalcin, osteopontin, osteonectin was apparent. These results demonstrate that the osteoblasts exhibit a full spectrum of cellular activity including the adequate differentiation on the PCA thin film. Therefore, a PCA thin film can be used as a coating material for biomaterials where the surface is not adequate for inducing the full activity of bone cells.
Hong
Ji-Yeon
JY
Laboratory for the Study of Molecular Biointerfaces, Department of Craniomaxillofacial Cell and Developmental Biology, College of Dentistry, South Korea.
Kim
Yoon Jung
YJ
Lee
Hee-Woo
HW
Lee
Woo-Kul
WK
Ko
Jea Seung
JS
Kim
Hyun-Man
HM
D016475:3T3 Cells; D000818:Animals; D001857:Bone Matrix / Q000502:physiology / Q000648:ultrastructure; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D003460:Crystallization / Q000379:methods*; D017886:Durapatite / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D008422:Materials Testing; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012886:Skull / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D013696:Temperature
D020099:Coated Materials, Biocompatible; D017886:Durapatite