Epstein-Barr virus nuclear antigen-1 colocalizes with lamin B1 in the nucleoplasm and along the nuclear rim.
Epstein-Barr virus nuclear antigen 1 (EBNA-1) is essential for the maintenance of latent EBV plasmids, and is also a transcriptional regulator. Nuclear lamins, components of the nuclear lamina, have also been found in the nucleoplasm. We report here that EBNA-1 coincided with lamin B1 in the nucleoplasm and around the nuclear rim during S-phase by confocal microscopy of cells transfected with EBNA-1 in the absence of EBV plasmids. Lamin B1, which is rarely detected in nuclear soluble fractions, was detected in chromatin and nuclear matrix fractions of the EBNA-1-expressing cells. These observations suggest that EBNA-1 colocalizes with lamin B1 in the subnuclear sites.
Ito
S
S
Department of Virology I, National Institute of Infectious Diseases, Tokyo, Japan.
Eda
H
H
Ban
F
F
Yanagi
K
K
D000818:Animals; D002051:Burkitt Lymphoma; D016466:CHO Cells; D002460:Cell Line; D002843:Chromatin / Q000378:metabolism; D006224:Cricetinae; D019309:Epstein-Barr Virus Nuclear Antigens / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D034921:Lamin Type B / Q000378:metabolism*; D015530:Nuclear Matrix / Q000378:metabolism*; D016196:S Phase; D014162:Transfection; D014407:Tumor Cells, Cultured
D002843:Chromatin; D019309:Epstein-Barr Virus Nuclear Antigens; D034921:Lamin Type B; C417029:EBV-encoded nuclear antigen 1