IL1-beta and TNF-alpha induce changes in the nuclear polyphosphoinositide signalling system in osteoblasts similar to that occurring in patients with rheumatoid arthritis: an immunochemical and immunocytochemical study.
Osteoarthritis (OA) and rheumatoid arthritis (RA) are common joint diseases that can lead to destruction of cartilage and structural changes in the subchondral bone. In this study we show by western blot and quantitative immunocytochemistry that nuclear phospholipase C beta(1) (PLC beta(1)) and phosphatidylinositol 4,5-bisphosphate (PIP(2)), two key elements of the polyphosphoinositide signal transduction system that regulate different cellular processes, increase in primary osteoblast cultures of RA patients when compared with post-traumatic after fall (PT) patients, whilst those of OA are not significantly affected. Moreover, we demonstrate that these alterations could be induced in PT osteoblasts by proinflammatory cytokines IL-1 beta and TNF-alpha. This suggests that proinflammatory cytokines, highly produced by RA infiltrating mononuclear cells, can modulate the nuclear polyphosphoinositide signalling pathway of the osteoblasts involved in bone remodelling.
Zini
Nicoletta
N
Sezioni di Bologna c/o IOR, ITOI-CNR, via di Barbiano 1/10, 40136 Bologna, Italy.
Lisignoli
Gina
G
Solimando
Liliana
L
Bavelloni
Alberto
A
Grassi
Francesco
F
Guidotti
Lia
L
Trimarchi
Carmela
C
Facchini
Andrea
A
Maraldi
Nadir Mario
NM
D001172:Arthritis, Rheumatoid / Q000201:enzymology*; D002467:Cell Nucleus / Q000201:enzymology; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007375:Interleukin-1 / Q000494:pharmacology*; D007527:Isoenzymes / Q000378:metabolism; D007867:Leg Bones / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010003:Osteoarthritis / Q000201:enzymology; D010006:Osteoblasts / Q000201:enzymology*; D054799:Phospholipase C beta; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D010738:Type C Phospholipases / Q000378:metabolism
D007375:Interleukin-1; D007527:Isoenzymes; D009419:Nerve Tissue Proteins; D014409:Tumor Necrosis Factor-alpha; C097702:synaptojanin; D010744:Phosphoric Monoester Hydrolases; D010738:Type C Phospholipases; C518033:PLCB1 protein, human; D054799:Phospholipase C beta