[Evaluation of fetal DNA in maternal plasma for prenatal diagnosis].
OBJECTIVE
To explore the application of fetal DNA in maternal plasma for uninvasive prenatal diagnosis.


METHODS
The DNA template was extracted by hydroxybenzene-chloroform from 44 maternal (7-41 weeks) plasma. A Y-chromosome-specific repeat sequence-DYZ1 gene (149 bp) was chosen to be amplified by PCR.


RESULTS
The fragment was identified in all the plasma of male bearing pregnant women. The diagnostic accordance rate was 100.00%; two of the 22 female bearing pregnant women had false positive results. Among the 44 pregnant women, the diagnostic accordance rate was 88.89% at early pregnant stage, 100.00% at medium pregnant stage, and 96.55% at late stage respectively. The final accuracy of 95.45% was obtained in all cases.


CONCLUSIONS
By means of hydroxybenzene-chloroform extraction the authors of this article promoted the concentration and purity of the DNA template, and diagnosed more accurately. The results showed that free fetal DNA in the maternal plasma could be regarded as the gene resource for uninvasive prenatal diagnosis.
Zhao
Yin
Y
The Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430022 PR China. zhaoyinzi@163.net
Zou
Li
L
D004247:DNA / Q000097:blood* / Q000235:genetics; D005260:Female; D005333:Fetus / Q000378:metabolism*; D006801:Humans; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D019849:Sex Determination Processes
D004247:DNA