title: Further investigation of the epitope recognized by the new monoclonal antibody 2C9.
abstract: OBJECTIVE
  We established a new monoclonal antibody (2C9) that reacted with prostate tissue. The immunohistochemical reactivity of this antibody is similar to anti-prostate-specific membrane antigen (PSMA). Herein, we report the antigenic determinant of 2C9 antibody.
  
  
  METHODS
  The reactivity of the antibody was characterized by immunohistochemical staining and the antigen target was characterized by amino acid sequencing after immuno-affinity purification from an LNCaP cell lysate and cloning of a cDNA using a mammalian expression cDNA cloning system.
  
  
  RESULTS
  The amino acid and nucleotide sequences for the antigen molecule recognized with 2C9 monoclonal antibody demonstrated identity with PSMA.
  
  
  CONCLUSIONS
  The target molecule of the 2C9 monoclonal antibody is PSMA, pointing to future diagnostic and therapeutic applications.
authors.affiliation: Department of Urology, Aichi Medical University School of Medicine, Nagakute, Aichi, Japan.
authors.forename: Keitaro
authors.initials: K
authors.lastname: Kato
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Yoshikawa
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Taki
authors.forename: Kenya
authors.initials: K
authors.lastname: Shitara
authors.forename: Kazuyasu
authors.initials: K
authors.lastname: Nakamura
authors.forename: Maiko
authors.initials: M
authors.lastname: Hirota
authors.forename: Nobuo
authors.initials: N
authors.lastname: Hanai
authors.forename: Kogenta
authors.initials: K
authors.lastname: Nakamura
authors.forename: Hiroto
authors.initials: H
authors.lastname: Kokubo
authors.forename: Kenji
authors.initials: K
authors.lastname: Mitsui
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Yamada
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Honda
authors.forename: Ryuzo
authors.initials: R
authors.lastname: Ueda
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Saga
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Fukatsu
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000235:genetics / Q000276:immunology*; D015152:Blotting, Northern; D045744:Cell Line, Tumor / Q000276:immunology / Q000378:metabolism; D002999:Clone Cells / Q000276:immunology; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D018604:Epitope Mapping / Q000379:methods; D000939:Epitopes / Q000032:analysis*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011467:Prostate / Q000737:chemistry / Q000276:immunology; D017430:Prostate-Specific Antigen / Q000032:analysis* / Q000737:chemistry; D011471:Prostatic Neoplasms / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D020539:Sequence Analysis, Protein
chemical_list: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D018076:DNA, Complementary; D000939:Epitopes; D017430:Prostate-Specific Antigen