[Effect of tissue-type plasminogen activator on differentiation of keratinocyte in the human embryonic epiderm].
OBJECTIVE
To explore the regulating roles of tissue-type plasminogen activator (tPA) in the differentiation of keratinocyte in the human epidermis.


METHODS
Immunocytochemistry and in situ hybridization were employed to study tPA expression quantitatively and qualitatively in early, middle and late human embryos skin respectively.


RESULTS
(1) The expression of tPA was significantly increased in the development of embryonic period, with the highest level in the middle embryonic phase, but the transcription of tPA still kept rather high level in the late human embryonic phase. (2) tPA was mainly localized in the superficial, more differentiated layers of the epidermis. (3) tPA was localized in peripheral cytoplasm of keratinocyte.


CONCLUSIONS
tPA participates the regulation of the keratinocyte differentiation.
Song
C
C
Department of Cell Biology, Third Military Medical University, Chongqing 400038, China. song_chuan@163.net
Yang
T
T
Yang
J
J
D002454:Cell Differentiation / Q000502:physiology; D000078404:Epidermal Cells; D004817:Epidermis / Q000196:embryology; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism*; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis* / Q000502:physiology
D010959:Tissue Plasminogen Activator