[Morphological and cytochemical properties of the human glioblastoma T98G cells during the monolayer culture].
The study was carried out with the aim of determination of morphological and cytochemical properties of human glioblastoma T98G cells in monolayer culture. Using light microscopic and immunocytochemical techniques, the glioblastoma cell line studied was shown to have low susceptibility to the effects of environmental factors and changes in culture conditions as well as high proliferative activity. All T98G cells were demonstrated to have positive nuclear reaction for p53 protein. An ability of T98G cells for spontaneous caspase 3-mediated apoptosis was shown using the antibodies against activated caspase 3. The data obtained suggest that glioblastoma T98G cell line could be used as a convenient model for testing the capacity of natural substances and of pharmaceutical agents that are being developed for the selective induction of caspase 3-mediated apoptosis.
Korzhevskiĭ
D E
DE
Department of Morphology, RAMS Institute of Experimental Medicine, St. Petersburg.
Otellin
V A
VA
Litvinchuk
L F
LF
D017209:Apoptosis; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D005909:Glioblastoma; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism
D005904:Glial Fibrillary Acidic Protein; D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen; D016159:Tumor Suppressor Protein p53; D014746:Vimentin; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases