title: Plasma concentrations of coenzyme Q10 and tocopherols in cervical intraepithelial neoplasia and cervical cancer.
abstract: Cervical intraepithelial neoplasia (CIN) may, at times, unpredictably progress to invasive carcinoma of the cervix. Epidemiological nutritional studies suggest that higher dietary consumption and circulating levels of certain micronutrients may be protective against cervical cancer. However, a preventive role of dietary antioxidants in CIN is not well established. The purpose of this cross-sectional study was to investigate the comparative plasma concentrations of three potent antioxidants, coenzyme Q(10,) alpha-tocopherol and gamma-tocopherol, in women with normal Pap smears and patients with a biopsy-confirmed histopathological lesion diagnosed as CIN or cervical cancer. Plasma concentrations of coenzyme Q(10,) alpha-tocopherol and gamma-tocopherol were measured by high-pressure liquid chromatography in both normal women without any history of abnormal Pap smears (n=48), and patients with histopathologically confirmed diagnoses of: (a) CIN I, n=98; (b) CIN II, n=49; (c) CIN III, n=10; and (d) cervical cancer, n=25. The mean plasma levels of coenzyme Q(10), alpha-tocopherol and gamma-tocopherol were significantly lower (P<0.001,<0.001, and<0.001, respectively by Kruskal-Wallis test) in patients with various grades of CIN and cervical cancer compared with controls. After controlling for age and smoking, an inverse association between histological grades of epithelial lesions and both plasma coenzyme Q(10) and alpha-tocopherol concentrations was observed. The low plasma concentrations of coenzyme Q(10) may be due to deficient dietary intake or a decrease in endogenous coenzyme Q(10) biosynthesis that may reflect increased utilization as a result of free radical reactive oxygen species induced oxidative stress. Further molecular studies on the mechanistic role of antioxidants in women with precancer cervical lesions are needed.
authors.affiliation: Department of Obstetrics and Gynecology, Bronx-Lebanon Hospital Center, 1650 Grand Concourse, Fifth Floor, Bronx, New York 10457, USA. daspalan@aol.com
authors.forename: P R
authors.initials: PR
authors.lastname: Palan
authors.forename: M S
authors.initials: MS
authors.lastname: Mikhail
authors.forename: D W
authors.initials: DW
authors.lastname: Shaban
authors.forename: S L
authors.initials: SL
authors.lastname: Romney
mesh_terms: D000293:Adolescent; D000328:Adult; D000975:Antioxidants / Q000032:analysis; D003067:Coenzymes; D003430:Cross-Sectional Studies; D004032:Diet; D005260:Female; D006801:Humans; D008875:Middle Aged; D012044:Regression Analysis; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000097:blood*; D002583:Uterine Cervical Neoplasms / Q000097:blood* / Q000517:prevention & control; D024502:alpha-Tocopherol / Q000097:blood*; D024504:gamma-Tocopherol / Q000097:blood*; D002578:Uterine Cervical Dysplasia / Q000097:blood* / Q000517:prevention & control
chemical_list: D000975:Antioxidants; D003067:Coenzymes; D014451:Ubiquinone; D024504:gamma-Tocopherol; C024989:coenzyme Q10; D024502:alpha-Tocopherol