title: Trans-kingdom conjugation in vitro by using toluenized cells.
abstract: To establish in vitro trans-kingdom conjugation, we developed a novel direct cell PCR by using toluenized cells which permeate enzymes, primers and substrates. The cells was shown to be successful recipients in conjugation.
authors.affiliation: Department of Biological Science, Graduate School of Science, Hiroshima University, Kagamiyama 1-3-1, Higashi-Hiroshima 739-8526, Japan.
authors.forename: Riko
authors.initials: R
authors.lastname: Tonomoto
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sonoda
authors.forename: Katsunori
authors.initials: K
authors.lastname: Suzuki
authors.forename: Kazuo
authors.initials: K
authors.lastname: Yoshida
mesh_terms: D001419:Bacteria / Q000235:genetics*; D003227:Conjugation, Genetic; D005658:Fungi / Q000235:genetics*; D010957:Plasmids; D016133:Polymerase Chain Reaction; D014050:Toluene / Q000008:administration & dosage*
chemical_list: D014050:Toluene