Formation and stability of alternate-stranded triplex formed with alpha,beta-chimeric oligoDNA.
Chimeric oligoDNAs composed of alpha-anomeric oligonucleotide and beta-anomeric oligonucleotide with modified nucleobases and/or phosphodiester backbone are synthesized and their ability to form alternate-stranded triple helix with dsDNA was examined. Substitution of normal phosphodiester linkages with phosphorothioate linkages resulted in the prominent destabilization of the alternate-stranded triplex. Meanwhile, the introduction of cationic molecule or intercalative molecule at certain positions of the modified DNA was effective to increase the stability of the triplexes.
Hirose
Yoshihiko
Y
Department of Chemistry, Faculty of Engineering, Gunma University, Kiryu 376-8515, Japan.
Kadoya
Aimi
A
Matsumoto
Noritake
N
Miyashita
Takanori
T
Moriguchi
Tomohisa
T
Shinozuka
Kazuo
K
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D009690:Nucleic Acid Conformation
D004247:DNA