Inhibition of photohemolysis induced by m-chloroperbenzoic acid by metal complexes with SOD-mimetic activity.
Red blood cells (RBCs) are probably the most common target through the damaging action of reactive oxygen species on the cells. The photohemolysis activity of m-chloroperbenzoic acid (CPBA) was concentration- and exposure time-dependent. Twenty minutes photo exposure time and 200 microm of CPBA concentration were optimum to study the effect of generated superoxide (O2-) and hydroxyl (*OH) radicals on RBCs. RBCs lysis photosensitized by CPBA was investigated in the presence of [(VL2O)(VL2H2O)]Cl6, [MnL2O]2Cl42H2O, [FeL2Cl2]Cl H2O, [CoL2Cl2]4H2O or [ZnL2Cl2]H2O respectively, where L is 2-methylaminopyridine, with SOD-mimetic activities with the aim of ascertaining their protective activity towards the photo induced cell damage. The decrease of photolytic activity caused by these complexes was concentration-dependent and the maximum percentage of protective activity was 75, 70, 68, 57 or 24% for [(VL2O)(VL2H2O)]Cl6, [MnL2O]2Cl4 2H2O, [FeL2Cl2]Cl H2O, [CoL2Cl2]4H2O or [ZnL2Cl2]H2O complex respectively, against the cell irradiated without addition of metal complexes. The comparison between the decrease of photolytic activity caused by these complexes and their SOD-mimetic activity of these metal complexes showed an appreciable correlation.
Abdel el-Aziz
Abdel el-Aziz F
Ael-A
Biochemistry Division, Chemistry Department, Faculty of Science, Mansoura University, Egypt.
el-Sayed
Ibrahim H
IH
D002462:Cell Membrane / Q000378:metabolism; D002723:Chlorobenzoates / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D005609:Free Radicals; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D008027:Light; D008670:Metals / Q000737:chemistry; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase / Q000737:chemistry*; D013997:Time Factors
D002723:Chlorobenzoates; D005609:Free Radicals; D008670:Metals; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase; C000433:3-chloroperbenzoic acid; D010100:Oxygen