title: Selection of aptamers against KMP-11 using colloidal gold during the SELEX process.
abstract: SELEX procedure is a methodology in which single stranded oligonucleotides are selected from a wide variety of sequences based on their interaction with a target molecule. We have designed a novel SELEX methodology using colloidal gold to select high affinity single stranded DNA aptamers against Leishmania infantum KMP-11. Kinetoplastid membrane protein-11 (KMP-11) is a major component of the cell membrane of kinetoplastid parasites. Although its function is not known, the fact that KMP-11 is a cytoskeleton-associated protein suggests that it may be involved in mobility or in some other aspects of the flagellar structure. We have isolated a single stranded DNA aptamer population that binds specifically to L. infantum KMP-11. This population has been characterized in a series of in vitro experiments suggesting that it may be used as a powerful tool to further investigate the role of KMP-11 during Leishmania development and/or as a diagnostic tool in Leishmania infection.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Universidad de Alcalá, 28871 Alcalá de Henares, Madrid, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: Moreno
authors.forename: Esther
authors.initials: E
authors.lastname: Rincón
authors.forename: David
authors.initials: D
authors.lastname: Piñeiro
authors.forename: Gerónimo
authors.initials: G
authors.lastname: Fernández
authors.forename: Alberto
authors.initials: A
authors.lastname: Domingo
authors.forename: Antonio
authors.initials: A
authors.lastname: Jiménez-Ruíz
authors.forename: Matilde
authors.initials: M
authors.lastname: Salinas
authors.forename: Víctor M
authors.initials: VM
authors.lastname: González
mesh_terms: D000818:Animals; D001483:Base Sequence; D016054:DNA, Protozoan / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D017979:Gold Colloid; D015151:Immunoblotting; D018314:Leishmania infantum / Q000235:genetics*; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D009838:Oligodeoxyribonucleotides / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D011485:Protein Binding; D015800:Protozoan Proteins / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D016054:DNA, Protozoan; D004277:DNA, Single-Stranded; D017979:Gold Colloid; D008562:Membrane Glycoproteins; D009838:Oligodeoxyribonucleotides; D015800:Protozoan Proteins; D011994:Recombinant Proteins; C090009:kinetoplastid membrane protein 11, Leishmania donovani