title: Sphinganine to sphingosine ratio and predictive biochemical markers of fumonisin B1 exposure in ducks.
abstract: The kinetics of free sphinganine (Sa), sphinganine to sphingosine ratio (Sa/So), proteins, cholesterol, alanine aminotransferase (ALAT) and lactate dehydrogenase (LDH) were investigated in the course of fumonisin B1 (FB1) exposure in ducks (20 growing males divided into four groups of 5 receiving, respectively, a daily dose of 0, 5, 15 or 45 mg/kg FB1 via oral administration over 12 days). Descriptive statistics of these parameters were also studied in a large number of ducks not exposed to mycotoxins and free of known pathology. Although the toxin at the end of the treatment affected all the parameters investigated, only 2 days of treatment appeared necessary to increase free Sa concentrations in serum, whereas 6 days were necessary to detect a significant effect on Sa/So ratio. Significant differences between control and treated ducks were observed after 4 days of treatment for ALAT and LDH and after 6 and 8 days for cholesterol and proteins concentrations. The minimum doses of FB1 required to determine an effect were assessed using three different methods. This approach reveals that FB1 has greater effects when it is ingested at a low dose for a long time than when ingested at a high dose for a short time. Although the minimum toxic dose of FB1 in ducks remains to be determined, this result must be considered in the context of chronic exposure to the toxin, not only in avian populations.
authors.affiliation: Department of Mycotoxicology, Veterinary School of Toulouse, 23 Chemin des Capelles, 31076 Toulouse, France.
authors.forename: S T
authors.initials: ST
authors.lastname: Tran
authors.forename: J D
authors.initials: JD
authors.lastname: Bailly
authors.forename: D
authors.initials: D
authors.lastname: Tardieu
authors.forename: S
authors.initials: S
authors.lastname: Durand
authors.forename: G
authors.initials: G
authors.lastname: Benard
authors.forename: P
authors.initials: P
authors.lastname: Guerre
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D015415:Biomarkers; D001798:Blood Proteins / Q000032:analysis; D002274:Carcinogens, Environmental / Q000493:pharmacokinetics*; D002784:Cholesterol / Q000097:blood; D004305:Dose-Response Relationship, Drug; D004372:Ducks; D037341:Fumonisins / Q000493:pharmacokinetics*; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D009183:Mycotoxins / Q000493:pharmacokinetics*; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000097:blood*
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; D002274:Carcinogens, Environmental; D037341:Fumonisins; D009183:Mycotoxins; C056933:fumonisin B1; D002784:Cholesterol; D007770:L-Lactate Dehydrogenase; D000410:Alanine Transaminase; D013110:Sphingosine; C005682:safingol