[Yeast one-hybrid system--one effective method studying DNA-protein interaction].
Yeast one-hybrid system based on the principle of yeast two-hybrid system, is applied for isolating novel genes encoding proteins that bind to a target, cis-acting regulatory element. The system may also be applied to map the DNA-binding domain of previously known, or newly identified DNA-binding proteins. Yeast one-hybrid system offers maximal sensitivity because detection of the DNA-protein interaction occurs while proteins are in their native configurations. In addition, the gene encoding the DNA-binding proteins of interest is immediately available after a library screening. In this mini-review, we introduce the principle, the experimental procedures, the applications, and the merits of yeast one-hybrid system.
Liao
M
M
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China.
Fang
F
F
D004247:DNA / Q000235:genetics; D011506:Proteins / Q000235:genetics; D020798:Two-Hybrid System Techniques; D015003:Yeasts / Q000235:genetics*
D011506:Proteins; D004247:DNA