Quantitative detection of binding of PCNA protein to DNA strands on a 27 MHz quartz-crystal microbalance.
We report here binding kinetics of proliferating cell nuclear antigen (PCNA) to the dsDNA immobilized on a 27 MHz quartz-crystal microbalance. PCNA is a toroidal-shaped protein and encircles dsDNA sliding along the strand. The binding behavior of this sliding clamp protein was compared with those of other side binding proteins such as GCN4-bZIP and Zn-finger peptides.
Okahata
Y
Y
Department of Biomolecular Engineering, Tokyo Institute of Technology, Nagatsuda, Midori-ku, Yokohama, Kanagawa 226-8501, Japan.
Kitamura
Y
Y
Hagiwara
N
N
Furusawa
H
H
D001483:Base Sequence; D050976:Basic-Leucine Zipper Transcription Factors; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D051790:G-Box Binding Factors; D066298:In Vitro Techniques; D007700:Kinetics; D009690:Nucleic Acid Conformation; D018809:Proliferating Cell Nuclear Antigen / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D011791:Quartz; D012996:Solutions; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism; D014867:Water; D016335:Zinc Fingers
D050976:Basic-Leucine Zipper Transcription Factors; D004268:DNA-Binding Proteins; D051790:G-Box Binding Factors; D018809:Proliferating Cell Nuclear Antigen; D012996:Solutions; D014157:Transcription Factors; D014867:Water; D011791:Quartz; D004247:DNA