Overexpression of the gene encoding GTP:mannose-1-phosphate guanyltransferase, mpg1, increases cellular GDP-mannose levels and protein mannosylation in Trichoderma reesei.
To elucidate the regulation and limiting factors in the glycosylation of secreted proteins, the mpg1 and dpm1 genes from Trichoderma reesei (Hypocrea jecorina) encoding GTP:alpha-D-mannose-1-phosphate guanyltransferase and dolichyl phosphate mannose synthase (DPMS), respectively, were overexpressed in T. reesei. No significant increases were observed in DPMS activity or protein secretion in dpm1-overexpressing transformants, whereas overexpression of mpg1 led to a twofold increase in GDP-mannose (GDPMan) levels. GDPMan was effectively utilized by mannnosyltransferases and resulted in hypermannosylation of secreted proteins in both N and O glycosylation. Overexpression of the mpg1 gene also increased the transcription of the dpm1 gene and DPMS activity. Our data indicate that the level of cellular GDPMan can play a major regulatory role in protein glycosylation in T. reesei.
Zakrzewska
Anna
A
Laboratory of Fungal Glycobiology, Institute of Biochemistry and Biophysics. Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Warsaw, Poland.
Palamarczyk
Grazyna
G
Krotkiewski
Hubert
H
Zdebska
Ewa
E
Saloheimo
Markku
M
Penttilä
Merja
M
Kruszewska
Joanna S
JS
D001426:Bacterial Proteins / Q000378:metabolism*; D006031:Glycosylation; D006155:Guanosine Diphosphate Mannose / Q000032:analysis*; D008358:Mannose / Q000378:metabolism*; D009713:Nucleotidyltransferases / Q000235:genetics*; D014242:Trichoderma / Q000235:genetics* / Q000378:metabolism*
D001426:Bacterial Proteins; D006155:Guanosine Diphosphate Mannose; D009713:Nucleotidyltransferases; C026917:mannose 1-phosphate guanylyltransferase; D008358:Mannose