title: Extracellular adenine nucleotides metabolism in astrocyte cultures from different brain regions.
abstract: Primary astrocyte cultures from hippocampus, cortex and cerebellum presented different extracellular pattern of adenine nucleotide hydrolysis. The ATP/ADP hydrolysis ratio was 8:1 for hippocampal and cortical astrocytes and 5:1 for cerebellar astrocytes. The AMP hydrolysis in cerebellar astrocytes was seven-fold higher than in cortical or hippocampal cells. No accumulation of extracellular adenosine in all structures studied was observed. Dipyridamol increased significantly inosine levels in the extracellular medium of hippocampal and cortical, but not in cerebellar astrocytes medium. A higher expression of ecto-5'-nucleotidase was identified by RT-PCR in cerebellum. The differences observed may indicate functional heterogeneity of nucleotides in the brain.
authors.affiliation: Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, UFRGS, Rua Ramiro Barcelos, 2600-anexo, CEP 90035-003, Porto Alegre, RS, Brazil.
authors.forename: Márcia R
authors.initials: MR
authors.lastname: Wink
authors.forename: Elizandra
authors.initials: E
authors.lastname: Braganhol
authors.forename: Alessandra S K
authors.initials: AS
authors.lastname: Tamajusuku
authors.forename: Emerson A
authors.initials: EA
authors.lastname: Casali
authors.forename: Juliana
authors.initials: J
authors.lastname: Karl
authors.forename: Maria L
authors.initials: ML
authors.lastname: Barreto-Chaves
authors.forename: João J F
authors.initials: JJ
authors.lastname: Sarkis
authors.forename: Ana M O
authors.initials: AM
authors.lastname: Battastini
mesh_terms: D015720:5'-Nucleotidase / Q000378:metabolism; D000227:Adenine Nucleotides / Q000378:metabolism*; D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000378:metabolism*; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002531:Cerebellum / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D004176:Dipyridamole / Q000494:pharmacology; D005110:Extracellular Space / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism; D033703:Nucleoside Transport Proteins / Q000037:antagonists & inhibitors; D009928:Organ Specificity; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000227:Adenine Nucleotides; D033703:Nucleoside Transport Proteins; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D004176:Dipyridamole; D000255:Adenosine Triphosphate; D015720:5'-Nucleotidase