[Cytosine diaminase/5-flurocytosine mediated inhibiting effect on the growth of human pancreatic carcinoma cells].
OBJECTIVE
To study the inhibition effect of E. coli cytosine diaminase (CD)/5-flurocytosine (5-FC) system on the growth of human pancreatic carcinoma cell line cells.


METHODS
Recombinant retroviral vector expressing CD genes was transduced into pancreatic carcinoma cell line cells. In vitro and in vivo prodrug sensitivity assays were carried out, including: (1) detection of growth and colony forming inhibition rate of transduced cells in the presence of 5-FC; (2) observation of bystander effect by MTT method; (3) observation of the growth inhibition effect of 5-FC on transduced cell xenografts in nude mice.


RESULTS
The growth and colony forming inhibition rate of the transduced cells in the presence of 5-FC were 80.0% and 79.0%, which were obviously higher than 4.8% and 5.0% (P < 0.01) of nontransduced cells. Mixed cells containing only 10% of transduced cells showed 50.7% reduction of proliferation (bystander effect). The growth of transduced cells xenografts could be totally regressed by 5-FC.


CONCLUSIONS
CD/5-FC system is a potential gene therapy strategy for pancreatic carcinoma.
Zhang
L
L
Department of Pathology, PUMC Hospital, CAMS, PUMC, Beijing 100730, China.
Liu
T
T
Cui
Q
Q
Gao
J
J
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D043525:Cytosine Deaminase / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology*; D005260:Female; D005437:Flucytosine / Q000494:pharmacology*; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010190:Pancreatic Neoplasms / Q000473:pathology / Q000628:therapy*; D011355:Prodrugs / Q000494:pharmacology*; D012190:Retroviridae / Q000235:genetics; D014407:Tumor Cells, Cultured
D011355:Prodrugs; D005437:Flucytosine; D043525:Cytosine Deaminase