title: Gene expression profile changes in NB4 cells induced by realgar.
abstract: OBJECTIVE
  To compare the gene expression profiles of acute promyelocytic leukemia cell line NB(4) before and after 12 hours of realgar treatment using cDNA microarray.
  
  
  METHODS
  Two cDNA probes were prepared through reverse transcription from mRNA of both untreated and realgar treated NB(4) cells. The probes were labeled with Cy3 and Cy5 fluorescence dyes individually, hybridized with cDNA microarray representing 1003 different human genes, and scanned for fluorescent intensity. The genes were screened through the analysis of the difference in two gene expression profiles.
  
  
  RESULTS
  The analysis of gene expression profiles indicates that 9 genes were up-regulated and 37 genes were down-regulated. Among the 9 up-regulated genes, 2 genes were involved in a proteasome degradation pathway. Some genes related to protein synthesis, signal transduction and cell receptors were down-regulated.
  
  
  CONCLUSIONS
  PSMC2 and PSMD1 genes may play an important role in the apoptosis and partial differentiation of NB(4) cells.
authors.affiliation: Department of Hematology, First Hospital of Xi'an Jiaotong University, Xi'an 710061, China. whuaiyu@sina.com
authors.forename: Huaiyu
authors.initials: H
authors.lastname: Wang
authors.forename: Shanxi
authors.initials: S
authors.lastname: Liu
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Lu
authors.forename: Xiao'ai
authors.initials: X
authors.lastname: Zhao
authors.forename: Siyu
authors.initials: S
authors.lastname: Chen
authors.forename: Xinmin
authors.initials: X
authors.lastname: Li
mesh_terms: D001152:Arsenicals / Q000494:pharmacology*; D015536:Down-Regulation; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D013440:Sulfides / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
chemical_list: D001152:Arsenicals; D013440:Sulfides; C058317:arsenic disulfide