Differences in cerebral reperfusion and oxidative injury after cardiac arrest in pigs.
BACKGROUND
An investigation of the free radical scavenger sodium 2-sulfophenyl-N-tert-butyl nitrone (S-PBN) and the weak vasodilatator Tris buffer mixture (TBM) on cerebral cortical blood flow (CCBF) and the jugular bulb concentration of two eicosanoids, indicators of oxidative stress and inflammation, was undertaken in 30 anaesthetized piglets during cardiopulmonary resuscitation (CPR) and after restoration of spontaneous circulation (ROSC).


METHODS
Thirty animals were subjected to 8 min of untreated circulatory arrest followed by 8 min of closed-chest CPR. During CPR, the animals were randomized to receive 60 mg/kg S-PBN, 1 mmol/kg TBM or 2 ml/kg normal saline (n = 10 in each group). Systemic haemodynamic variables, CCBF and jugular bulb plasma concentrations of 8-iso-PGF2alpha and 15-keto-dihydro-PGF2alpha were measured.


RESULTS
The CCBF during reperfusion after ROSC was greater in the TBM group than in the S-PBN group, the regression coefficient between CCBF and mean arterial blood pressure being lower in the S-PBN group than in the TBM group. The jugular bulb plasma concentration of 8-iso-PGF2alpha during the first 30 min after ROSC was greater in the TBM group than in the S-PBN group. Administration of TBM after vasopressin did not attenuate the pressor effect of vasopressin.


CONCLUSIONS
Administration of S-PBN during CPR results in less cerebral oxidative stress, possibly by promoting normal distribution of cerebral blood flow.
Liu
X L
XL
Departments of Anaesthesiology and Intensive Care and Public Health/Geriatrics, Uppsala University Hospital, Uppsala, Sweden.
Wiklund
L
L
Nozari
A
A
Rubertsson
S
S
Basu
S
S
D000818:Animals; D001794:Blood Pressure; D001921:Brain / Q000378:metabolism; D016887:Cardiopulmonary Resuscitation; D002560:Cerebrovascular Circulation; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000097:blood; D028441:F2-Isoprostanes / Q000097:blood; D005260:Female; D006323:Heart Arrest / Q000503:physiopathology*; D006863:Hydrogen-Ion Concentration; D008297:Male; D018384:Oxidative Stress; D015427:Reperfusion Injury / Q000209:etiology*; D013552:Swine
D028441:F2-Isoprostanes; C008228:15-keto-13,14-dihydroprostaglandin F2alpha; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost