[Virulence of Yersinia pseudotuberculosis strains isolated from environment as a threat to human epidemic].
The purpose of the work has been to identify Yersinia pseudotuberculosis strains and to demonstrate their potential pathogenicity using PCR reaction. Investigations included 167 samples of the water and 32 samples of the soil from westpomeranian region. Based on the analysis of PCR reactions the research confirmed the presence of DNA Y. pseudotuberculosis strain in 6 of the water samples and in 1 of the soil sample. The strains have been identified by nucleotide sequence of the ypm gene, which is specific only for mentioned species. Genotypic analysis demonstrated also the presence of genes, which confirmed their virulence. The PCR reaction should be used in the microbiological diagnostic of Y. pseudotuberculosis strains, isolated from animals and from environment, because it is very specific, fast and sensitive method.
Czyzewska
Izabela
I
Katedra Immunologii i Mikrobiologii Wydziału Biotechnologii i Hodowli Zwierzat Akademii Rolniczej w Szczecinic.
Furowicz
Antoni J
AJ
D001426:Bacterial Proteins / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D004196:Disease Outbreaks / Q000517:prevention & control*; D004777:Environment; D006801:Humans; D016133:Polymerase Chain Reaction; D012988:Soil Microbiology; D014871:Water Microbiology; D015011:Yersinia pseudotuberculosis / Q000235:genetics / Q000302:isolation & purification*; D015012:Yersinia pseudotuberculosis Infections / Q000453:epidemiology* / Q000235:genetics / Q000382:microbiology*
D001426:Bacterial Proteins; D004279:DNA, Viral; C093313:Yersinia pseudotuberculosis-derived mitogen