Imaging polarimetry for high throughput chiral screening.
Imaging polarimetry was demonstrated as a highly parallel method of determining optical rotation of biochemical samples. The imaging polarimeter utilized a bright, uniform light source wavelength-filtered to near the sodium D line, a sample array flanked by inlet and analyzing polarizers, and a CCD camera fitted with an equal-perspective telecentric lens. The prototype apparatus was demonstrated to have an optical resolution better than 0.08 degrees. The potential for high throughput screening was demonstrated by imaging chiral solutions in 1536-well microtiter plates and by real-time monitoring of 30 simultaneous chiral enzymatic reactions. Improvements in polarizer and CCD technology may broadly expand the technique's applicability to fields such as directed evolution and combinatorial chemistry, where screening throughput is currently limiting for chiral applications.
Gibbs
Phillip R
PR
Department of Chemical Engineering, University of Houston, Houston, Texas 77204-4792, USA.
Uehara
Christian S
CS
Nguyen
Peter T
PT
Willson
Richard C
RC
D000465:Algorithms; D020650:Combinatorial Chemistry Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005240:Feasibility Studies; D005947:Glucose / Q000737:chemistry*; D005964:Glucosyltransferases / Q000737:chemistry*; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D008859:Microscopy, Polarization / Q000295:instrumentation* / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013237:Stereoisomerism
D005964:Glucosyltransferases; C116853:sucrose isomerase; D005947:Glucose