title: Genome-wide insertional mutagenesis of Arabidopsis thaliana.
abstract: Over 225,000 independent Agrobacterium transferred DNA (T-DNA) insertion events in the genome of the reference plant Arabidopsis thaliana have been created that represent near saturation of the gene space. The precise locations were determined for more than 88,000 T-DNA insertions, which resulted in the identification of mutations in more than 21,700 of the approximately 29,454 predicted Arabidopsis genes. Genome-wide analysis of the distribution of integration events revealed the existence of a large integration site bias at both the chromosome and gene levels. Insertion mutations were identified in genes that are regulated in response to the plant hormone ethylene.
authors.affiliation: Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
authors.forename: José M
authors.initials: JM
authors.lastname: Alonso
authors.forename: Anna N
authors.initials: AN
authors.lastname: Stepanova
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Leisse
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Kim
authors.forename: Huaming
authors.initials: H
authors.lastname: Chen
authors.forename: Paul
authors.initials: P
authors.lastname: Shinn
authors.forename: Denise K
authors.initials: DK
authors.lastname: Stevenson
authors.forename: Justin
authors.initials: J
authors.lastname: Zimmerman
authors.forename: Pascual
authors.initials: P
authors.lastname: Barajas
authors.forename: Rosa
authors.initials: R
authors.lastname: Cheuk
authors.forename: Carmelita
authors.initials: C
authors.lastname: Gadrinab
authors.forename: Collen
authors.initials: C
authors.lastname: Heller
authors.forename: Albert
authors.initials: A
authors.lastname: Jeske
authors.forename: Eric
authors.initials: E
authors.lastname: Koesema
authors.forename: Cristina C
authors.initials: CC
authors.lastname: Meyers
authors.forename: Holly
authors.initials: H
authors.lastname: Parker
authors.forename: Lance
authors.initials: L
authors.lastname: Prednis
authors.forename: Yasser
authors.initials: Y
authors.lastname: Ansari
authors.forename: Nathan
authors.initials: N
authors.lastname: Choy
authors.forename: Hashim
authors.initials: H
authors.lastname: Deen
authors.forename: Michael
authors.initials: M
authors.lastname: Geralt
authors.forename: Nisha
authors.initials: N
authors.lastname: Hazari
authors.forename: Emily
authors.initials: E
authors.lastname: Hom
authors.forename: Meagan
authors.initials: M
authors.lastname: Karnes
authors.forename: Celene
authors.initials: C
authors.lastname: Mulholland
authors.forename: Ral
authors.initials: R
authors.lastname: Ndubaku
authors.forename: Ian
authors.initials: I
authors.lastname: Schmidt
authors.forename: Plinio
authors.initials: P
authors.lastname: Guzman
authors.forename: Laura
authors.initials: L
authors.lastname: Aguilar-Henonin
authors.forename: Markus
authors.initials: M
authors.lastname: Schmid
authors.forename: Detlef
authors.initials: D
authors.lastname: Weigel
authors.forename: David E
authors.initials: DE
authors.lastname: Carter
authors.forename: Trudy
authors.initials: T
authors.lastname: Marchand
authors.forename: Eddy
authors.initials: E
authors.lastname: Risseeuw
authors.forename: Debra
authors.initials: D
authors.lastname: Brogden
authors.forename: Albana
authors.initials: A
authors.lastname: Zeko
authors.forename: William L
authors.initials: WL
authors.lastname: Crosby
authors.forename: Charles C
authors.initials: CC
authors.lastname: Berry
authors.forename: Joseph R
authors.initials: JR
authors.lastname: Ecker
mesh_terms: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000483:Alleles; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D001482:Base Composition; D032461:Chromosomes, Plant / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D005030:Ethylenes / Q000494:pharmacology; D005091:Exons; D020224:Expressed Sequence Tags; D015870:Gene Expression; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D018745:Genome, Plant; D007438:Introns; D016254:Mutagenesis, Insertional; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D012231:Rhizobium / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D029681:Arabidopsis Proteins; D004269:DNA, Bacterial; D018744:DNA, Plant; D005030:Ethylenes; C036483:T-DNA; C036216:ethylene