title: Application of statistically-based experimental designs for the optimization of nisin production from whey.
abstract: Statistically-based experimental designs were applied for the optimization of nisin production by Lactococcus lactis in a whey-based medium. Yeast extract, KH2PO4, and MgSO4 were identified to have significant effects on nisin biosynthesis by a Plackett-Burman design. These three significant factors were subsequently optimized using central composite design, and the optimal conditions were determined to be 12.067 g l(-1) for yeast extract, 0.569 g l(-1) for KH2PO4, and 0.572 g l(-1) for MgSO4. The validity of the optimal conditions was verified by a separate experiment.
authors.affiliation: Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164-6120, USA.
authors.forename: Chuanbin
authors.initials: C
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Liao
authors.forename: Zhiyou
authors.initials: Z
authors.lastname: Wen
authors.forename: Shulin
authors.initials: S
authors.lastname: Chen
mesh_terms: D000818:Animals; D019149:Bioreactors / Q000382:microbiology*; D003470:Culture Media / Q000494:pharmacology; D005163:Factor Analysis, Statistical; D019344:Lactic Acid / Q000096:biosynthesis; D013294:Lactococcus lactis / Q000145:classification / Q000378:metabolism*; D008278:Magnesium Sulfate / Q000494:pharmacology; D008892:Milk / Q000378:metabolism* / Q000382:microbiology; D008954:Models, Biological; D015233:Models, Statistical; D009561:Nisin / Q000096:biosynthesis*; D010710:Phosphates / Q000494:pharmacology; D017680:Potassium Compounds / Q000494:pharmacology; D011786:Quality Control; D012680:Sensitivity and Specificity; D013045:Species Specificity
chemical_list: D003470:Culture Media; D010710:Phosphates; D017680:Potassium Compounds; D009561:Nisin; D019344:Lactic Acid; D008278:Magnesium Sulfate; C013216:potassium phosphate