Common genes responsible for differentiation and senescence of human mucosal and epidermal keratinocytes.
Serial subcultures of normal human oral keratinocytes (NHOKs) and normal human epidermal keratinocytes (NHEKs) to the postmitotic stage result in terminal differentiation and replicative senescence. In order to investigate the common mode of differentiation and/or senescence between mucosal and epidermal keratinocytes, gene expression profiling on both NHOKs and NHEKs was performed by a cDNA microarray analysis. Primary NHOKs and NHEKs were serially subcultured, and the expression level of 3,063 genes was compared between the exponentially growing and senescent cultures. The senescent NHOKs and NHEKs highly expressed 55 and 37 genes, respectively. Among these genes, 16 genes were common in both NHOKs and NHEKs while the other genes were upregulated either in the NHOKs or in the NHEKs. Furthermore, the expression levels of the common genes did not change in the human diploid fibroblasts during the subcultures. These results suggest that subculture-induced differentiation and/or replicative senescence in NHOKs and NHEKs has similar characteristics, but that the pathways leading to these processes are distinct and keratinocyte specific.
Baek
Jeong-Hwa
JH
Department of Pharmacology and Dental Therapeutics, Dental Research Institute, Seoul, Korea.
Lee
Gene
G
Kim
Si Nae
SN
Kim
Jin-Man
JM
Kim
Moonkyu
M
Chung
Sung-Chang
SC
Min
Byung-Moo
BM
D015152:Blotting, Northern; D002454:Cell Differentiation / Q000235:genetics* / Q000502:physiology; D016922:Cellular Senescence / Q000235:genetics* / Q000502:physiology; D004817:Epidermis / Q000502:physiology; D006801:Humans; D015603:Keratinocytes / Q000502:physiology*; D009092:Mucous Membrane / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis