title: Tandem oligonucleotide synthesis using linker (First Base) phosphoramidites.
abstract: First Base linker phosphoramidites add the first nucleoside to underivatized solid-phase supports using a regular phosphoramidite coupling cycle. A cleavable 3'-O-ester linkage allows NH4OH treatment to rapidly generate products with only 3'-OH ends. Either single or multiple sequences linked in tandem can be prepared and no additional deprotection steps are required.
authors.affiliation: University of Calgary, Department of Biochemistry and Molecular Biology, Calgary, AB, Canada T2N 4N1.
authors.forename: Richard T
authors.initials: RT
authors.lastname: Pon
authors.forename: Shuyuan
authors.initials: S
authors.lastname: Yu
mesh_terms: D000577:Amides / Q000737:chemistry*; D019075:Electrophoresis, Capillary; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D010756:Phosphoric Acids / Q000737:chemistry*
chemical_list: D000577:Amides; D009841:Oligonucleotides; D010756:Phosphoric Acids; C011067:phosphoramidic acid