Assessment of the effect of Punica granatum (pomegranata) on the bioavailability of the radiopharmaceutical sodium pertechnetate (99mTc) in Wistar rats.
The many desirable characteristics of technetium-99m (99mTc) have stimulated the development of labeling techniques for different molecular and cellular structures. It is generally accepted that a variety of factors other than disease can alter the bioavailability of radiopharmaceuticals and one such factor is the drug therapy. The use of medicinal plants has increased in the last decades all over the world. Punica granatum (pomegranata) is used as food or as medication in folk medicine for antiviral, anthelmintic, antifungal, antibacterial and antimicrobial activity. We have studied in rats, the effect of the medicinal plant Punica granatum on the bioavailability of the radiopharmaceutical 99mTc-sodium pertechnetate (Na(99m)TcO4). The infusion of pomegranata was administered by intragastric via into Wistar rats during seven days. After that, the animals received by ocular plexus via, 0.1 ml of the Na(99m)TcO4 (3.7MBq) and the animals were rapidly sacrificed after 5, 20 and 40 min. The organs were isolated (brain, heart, thyroid, liver, lungs, kidneys, stomach, testis, intestines, pancreas, spleen, bladder, muscle and bone), the radioactivity determined in a well counter, the percentages of radioactivity (%ATI) in the organs were calculated and statistical analyses were performed by Wilcoxon test (p < 0.05). The results have shown a significant (p < 0.05) increase of the activity of the Na(99m)TcO4 in spleen, heart, stomach, liver, stout bowel, pancreas, lungs and testis at 5 min. Twenty minutes after the administration of the radiopharmaceutical, the analysis of the results reveals a significant (p < 0.05) increase of the %ATI in heart, stomach, femur, pancreas, lungs and kidneys. Forty minutes after the administration of the Na(99m)TcO4, the results show a significant (p < 0.05) increase in spleen, brain, heart, stomach, liver, stout bowel, muscle, femur, lungs, pancreas, kidneys and testis. These results can be justified by therapeutic effect of this extract and/or by generation of active metabolites capable to interfere with the biodistribution of the studied radiopharmaceutical.
Amorim
L F
LF
Universidade Federal do Rio Grande do Norte, Centro de Biociências, Depto de Fisiologia. Av. Salgado Filho, 3000, Natal, RN 59078-970, Brazil. lfa@ufrnet.br
Catanho
M T J A
MT
Terra
D A
DA
Brandão
K C
KC
Holanda
C M C X
CM
Jales-Júnior
L H
LH
Brito
L M L
LM
Gomes
M L
ML
De Melo
V G B
VG
Bernardo-Filho
M
M
Cavalcanti Jales
R L
RL
D000818:Animals; D029561:Lythraceae / Q000378:metabolism*; D009928:Organ Specificity; D010936:Plant Extracts / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013670:Sodium Pertechnetate Tc 99m / Q000378:metabolism*
D010936:Plant Extracts; D013670:Sodium Pertechnetate Tc 99m