CHD1 associates with NCoR and histone deacetylase as well as with RNA splicing proteins.
CHD1 is one of a family of nuclear proteins containing two chromodomains, a SWI/SNF-like helicase/ATPase domain and a DNA binding domain. We found that CHD1 co-immunoprecipitates with histone deacetylase (HDAC) activity and that CHD1 also associates with NCoR, a transcriptional corepressor, in yeast two-hybrid and in vitro pull-down assays. NCoR is known to associate with HDACs to effect its repressive activity, suggesting that the predicted chromatin remodeling activity of CHD1 plays a role in this repression. Yeast two-hybrid assays also showed that CHD1 interacts with splicing proteins mKIAA0164, Srp20, and SAF-B. Splicing assays show that CHD1 overexpression can affect alternative splicing. These results suggest that CHD1 may function in both chromatin mediated transcriptional repression and RNA splicing.
Tai
Helen H
HH
Ottawa Regional Cancer Centre, and Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ont., Canada. htai@nrcan.gc.ca
Geisterfer
Margit
M
Bell
John C
JC
Moniwa
Mariko
M
Davie
James R
JR
Boucher
Lorrie
L
McBurney
Michael W
MW
D001483:Base Sequence; D002460:Cell Line; D004265:DNA Helicases; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000378:metabolism*; D006655:Histone Deacetylases / Q000378:metabolism*; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism*; D056971:Nuclear Receptor Co-Repressor 1; D011485:Protein Binding; D012326:RNA Splicing; D012097:Repressor Proteins / Q000378:metabolism*
D017931:DNA Primers; D004268:DNA-Binding Proteins; C539127:NCOR1 protein, human; D009687:Nuclear Proteins; D056971:Nuclear Receptor Co-Repressor 1; D012097:Repressor Proteins; D006655:Histone Deacetylases; D004265:DNA Helicases; C080359:CHD1 protein, human