[Differential gene expression of liver carcinoma cell after transfection of by ARL-1 with microarray].
OBJECTIVE
To study the difference of the gene expression profile and to identify the different expression after transfection of the ARL-1 gene.


METHODS
The cDNA probes were synthesized from total RNA of study group and control group, which was differentially hybridized to cDNA chips and confirmed by a gene specific semiquantitative reverse transcription polymerase chain reaction (RT-PCR).


RESULTS
Six kinds of gene expression were increased and 9 kinds of gene expression were decreased. The findings were correlated with protein metabolism, signal pathway, metastasis, and drug resistance.


CONCLUSIONS
cDNA chips showed that gene expression profile of liver carcinoma cell was changed after transfection of the ARL-1 gene. It is a useful method in understanding the mechanism of drug resistance.
Tang
Da-nian
DN
Department of Surgery, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Li
Yong-guo
YG
Wei
Jun-min
JM
Yang
Xiang-dong
XD
Zhu
Min-wei
MW
Wu
Pei
P
Miao
Xiong-ying
XY
D000449:Aldehyde Reductase / Q000235:genetics*; D000074408:Aldo-Keto Reductases; D019008:Drug Resistance, Neoplasm; D020869:Gene Expression Profiling; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D014162:Transfection
C120471:AKR1B10 protein, human; D000074408:Aldo-Keto Reductases; D000449:Aldehyde Reductase