Cryo-atomic force microscopy of unphosphorylated and thiophosphorylated single smooth muscle myosin molecules.
The purpose of this study was to determine whether steric blockage of one head by the second head of native two-headed myosin was responsible for the inactivity of nonphosphorylated two-headed myosin compared with the high activity of single-headed myosin, as suggested on the basis of electron microscopy of two-dimensional crystals of heavy meromyosin (Wendt, T., Taylor, D., Messier, T., Trybus, K. M., and Taylor, K. A. (1999) J. Cell Biol. 147, 1385-1390; and Wendt, T., Taylor, D., Trybus, K. M., and Taylor, K. (2001) Proc. Natl. Acad. Sci. U. S. A. 98, 4361-4366). Our earlier cryo-atomic force microscopy (cryo-AFM) (Zhang, Y., Shao, Z., Somlyo, A. P., and Somlyo, A. V. (1997) Biophys. J. 72, 1308-1318) indicates that thiophosphorylation of the regulatory light chain increases the separation of the two heads of a single myosin molecule, but the thermodynamic probability of steric hindrance by strong binding between the two heads was not determined. We now report this probability determined by cryo-AFM of single whole myosin molecules shown to have normal low ATPase activity (0.007 s-1). We found that the thermodynamic probability of the relative head positions of nonphosphorylated myosin was approximately equal between separated heads as compared with closely apposed heads (energy difference of 0.24 kT (where k is a Boltzman constant and T is the absolute temperature)), and thiophosphorylation increased the number of molecules having separated heads (energy advantage of -1.2 kT (where k is a Boltzman constant and I is the absolute temperature)). Our results do not support the suggestion that strong binding of one head to the other stabilizes the blocked conformation against thermal fluctuations resulting in steric blockage that can account for the low activity of nonphosphorylated two-headed myosin.
Sheng
Sitong
S
Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, Virginia 22908-0736, USA.
Gao
Yan
Y
Khromov
Alexander S
AS
Somlyo
Avril V
AV
Somlyo
Andrew P
AP
Shao
Zhifeng
Z
D000818:Animals; D005615:Freezing; D066298:In Vitro Techniques; D018625:Microscopy, Atomic Force / Q000379:methods; D009130:Muscle, Smooth / Q000378:metabolism; D010766:Phosphorylation; D011487:Protein Conformation; D024745:Smooth Muscle Myosins / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure*; D013816:Thermodynamics; D014422:Turkeys
D024745:Smooth Muscle Myosins