Identification of a human cDNA sequence which encodes a novel membrane-associated protein containing a zinc metalloprotease motif.
We report the cloning and characterization of a human cDNA predicted to encode a novel hydrophobic protein containing four transmembrane domains and a zinc metalloprotease motif, HEXXH, between the third and fourth transmembrane domains, and have named the molecule metalloprotease-related protein-1 (MPRP-1). The MPRP-1 gene was localized to chromosome 1-p32.3 by radiation hybrid mapping, and Northern blot analysis revealed expression in many organs, with strong expression in the heart, skeletal muscle, kidney and liver. Immunohistochemical analyisis showed that MPRP-1 was localized in the endoplasmic reticulum (ER), and not in the Golgi compartment. Fragments of DNA encoding a segment homologous to the HEXXH motif of MPRP-1 are widely found in bacteria, yeast, plants, and animals. These results suggest that the MPRP-1 may have highly conserved functions, such as in intracellular proteolytic processing in the ER.
Bao
Ying-Chun
YC
Division of Cell Therapy, Division of Hematopoietic Factors, The Advanced Clinical Research Center, The Institute of Medical Science, University of Tokyo, 6-1 Shirokanedai 4-chome, Minato-ku, Tokyo 108-8639, Japan.
Tsuruga
Hiromichi
H
Hirai
Momoki
M
Yasuda
Kazuki
K
Yokoi
Norihide
N
Kitamura
Toshio
T
Kumagai
Hidetoshi
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D004721:Endoplasmic Reticulum / Q000201:enzymology; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008666:Metalloendopeptidases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D015032:Zinc
D018076:DNA, Complementary; D008565:Membrane Proteins; D008666:Metalloendopeptidases; C477817:molecule metalloprotease-related protein-1, human; D015032:Zinc