[Expression of rat protamine gene in MEL cells].
OBJECTIVE
To investigate the expression of rat protamine (RP) gene in MEL cells and the effect on cell growth.


METHODS
Eukaryotic expression plasmid pCR-3.1-RP was constructed and transfected into MEL cells. The changes of cell growth rate, mitotic index and colony-forming rate in semi-solid medium were investigated.


RESULTS
Transfected MEL cells showed lower growth rate, mitotic index and colony-forming rate. Volumes of cells were reduced and reduction of RNA transcription was observed.


CONCLUSIONS
These results suggest that expression of RP in MEL cell may inhibit the cell growth and proliferation.
Chen
Yu
Y
Institute of Materia Medica, CAMS, PUMC, Beijing 100050, China.
Fei
Ren-ren
RR
Wu
Xiao-fang
XF
Ge
Ye-hua
YH
Chen
Hui
H
Ma
Jing
J
Han
Dai-shu
DS
Xue
She-pu
SP
D000818:Animals; D002455:Cell Division; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics* / Q000473:pathology; D010957:Plasmids; D011479:Protamines / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D014162:Transfection; D014407:Tumor Cells, Cultured
D011479:Protamines