title: Dynamic aspects of granulocyte activation in rat severe acute pancreatitis.
abstract: We demonstrated dynamic aspects of granulocyte activation in rat severe acute pancreatitis, which was induced by cerulein and aggravated following lipopolysaccharide (LPS) injection. Pancreatitis induced by cerulein increased intracellular elastase activity of granulocytes in the blood. However, significant systemic cytokinemia was not provoked under such conditions. After induction of severe pancreatitis by LPS, intracellular elastase activity of circulating granulocytes decreased markedly and immediately. This decrease occurred simultaneous to induction of systemic hypercytokinemia and granulocyte migration into the lung. Overall results imply that: (1) circulating granulocytes are activated by induction of mild pancreatitis; (2) activation of granulocytes is mediated by factors other than systemic cytokinemia, such as locally produced cytokines; (3) those priming granulocytes immediately and significantly migrate from the circulation into the extravascular space by induction of endotoxemia; and (4) migration of granulocytes, in turn, may be mediated by systemic cytokinemia.
authors.affiliation: Department of Surgery II, Kumamoto University Medical School, 1-1-1 Honjo, Kumamoto-city, Kumamoto 860-8556, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Kimura
authors.forename: Masahiko
authors.initials: M
authors.lastname: Hirota
authors.forename: Akihiro
authors.initials: A
authors.lastname: Okabe
authors.forename: Kotaro
authors.initials: K
authors.lastname: Inoue
authors.forename: Kinuko
authors.initials: K
authors.lastname: Kuwata
authors.forename: Masaki
authors.initials: M
authors.lastname: Ohmuraya
authors.forename: Michio
authors.initials: M
authors.lastname: Ogawa
mesh_terms: D000208:Acute Disease; D000818:Animals; D002108:Ceruletide; D016207:Cytokines / Q000097:blood; D006098:Granulocytes / Q000201:enzymology / Q000276:immunology*; D007375:Interleukin-1 / Q000097:blood; D007958:Leukocyte Count; D008070:Lipopolysaccharides; D008168:Lung / Q000201:enzymology / Q000276:immunology; D008297:Male; D010196:Pancreatic Elastase / Q000378:metabolism; D010195:Pancreatitis / Q000097:blood* / Q000139:chemically induced; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016207:Cytokines; D007375:Interleukin-1; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D002108:Ceruletide; D009195:Peroxidase; D010196:Pancreatic Elastase