Analysis of allelic expression patterns of IL-2, IL-3, IL-4, and IL-13 in human CD4+ T cell clones.
The occurrence of monoallelic expression of cytokine genes in single cells has been convincingly demonstrated, but there have been few reports of this phenomenon in T cell clones. Here we describe studies on the expression of alleles of the human genes encoding IL-2, IL-3, IL-4, and IL-13 in human CD4(+) T cell clones. In contrast to the results reported in mouse T cell clones and single human T cells, we found no evidence for the monoallelic expression of the IL-2, IL-3, and IL-13 genes. The gene for IL-4 showed an imbalance in expression from each allele, indicating differential expression of IL-4 alleles within or between IL-4-expressing cells.
Bayley
Jean-Pierre
JP
Department of Rheumatology, Leiden University Medical Center, Leiden, The Netherlands.
Bakker
Aleida M
AM
Kaijzel
Eric L
EL
Wierenga
Eddy A
EA
van der Pouw-Kraan
Tineke C T M
TC
Huizinga
Tom W J
TW
Verweij
Cornelis L
CL
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002999:Clone Cells; D015870:Gene Expression; D005838:Genotype; D006579:Heterozygote; D006801:Humans; D018793:Interleukin-13 / Q000235:genetics; D007376:Interleukin-2 / Q000235:genetics; D007377:Interleukin-3 / Q000235:genetics; D015847:Interleukin-4 / Q000235:genetics; D007378:Interleukins / Q000235:genetics*; D051379:Mice; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide
D018793:Interleukin-13; D007376:Interleukin-2; D007377:Interleukin-3; D007378:Interleukins; D015847:Interleukin-4