Comparison of morphine and hydromorphone analysis on reversed phase columns with different diameters.
A comparison of a reversed phase high-performance liquid chromatographic (HPLC) method performed on columns with different internal diameters is reported for the quantitative routine determination of morphine.HCl and hydromorphone.HCl in solutions used for intramuscular injection. The method is based on the ion-pairing properties of sodium dodecyl sulphate (SDS) with alkaloids on a reversed phase LiChrospher RP-18 packing material and UV-detection at 230 nm. The mobile phase consisted of an acetonitrile: water mixture 35:65 (v/v) containing 0.5% (w/v) SDS and 0.4% (v/v) acetic acid. Precision, linearity and limit of detection were compared on the 2, 3 and 4 mm i.d. x 125 mm columns. A robustness test for the determination of hydromorphone.HCl was also evaluated.
Baeyens
W R G
WR
Laboratory of Drug Quality Control, Department of Pharmaceutical Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, B-9000 Ghent, Belgium. willy.baeyens@UGent.be
Van der Weken
G
G
Smet
E
E
García-Campaña
A M
AM
Remon
J P
JP
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D004091:Hydromorphone / Q000032:analysis* / Q000737:chemistry; D009020:Morphine / Q000032:analysis* / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000295:instrumentation / Q000379:methods*
D009020:Morphine; D004091:Hydromorphone