title: Homocysteine oxidation and apoptosis: a potential cause of cleft palate.
abstract: Cleft palate is the most common craniofacial anomaly. Affected individuals require extensive medical and psychosocial support. Although cleft palate has a complex and poorly understood etiology, low maternal folate is known to be a risk factor for craniofacial anomalies. Folate deficiency results in elevated homocysteine levels, which may disturb palatogenesis by several mechanisms, including oxidative stress and perturbation of matrix metabolism. We examined the effect of homocysteine-induced oxidative stress on human embryonic palatal mesenchyme (HEPM) cells and demonstrated that biologically relevant levels of homocysteine (20-100 microM) with copper (10 microM) resulted in dose-dependent apoptosis, which was prevented by addition of catalase but not superoxide dismutase. Incubation of murine palates in organ culture with homocysteine (100 micro) and CuSO(4) (10 microM) resulted in a decrease in palate fusion, which was not significant. Gelatin gel zymograms of HEPM cell-conditioned media and extracts of cultured murine palates, however, showed no change in the expression or activation of pro-matrix metalloproteinase-2 with homocysteine (20 microM-1 mM) with or without CuSO(4) (10 microM). We have demonstrated that biologically relevant levels of homocysteine in combination with copper can result in apoptosis as a result of oxidative stress; therefore, homocysteine has the potential to disrupt normal palate development.
authors.affiliation: Division of Child Dental Health, Dental School, University of Bristol, United Kingdom BS1 2LY. l.knott@bris.ac.uk
authors.forename: Lynda
authors.initials: L
authors.lastname: Knott
authors.forename: Tom
authors.initials: T
authors.lastname: Hartridge
authors.forename: Nathan L
authors.initials: NL
authors.lastname: Brown
authors.forename: Jason P
authors.initials: JP
authors.lastname: Mansell
authors.forename: Jonathon R
authors.initials: JR
authors.lastname: Sandy
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D020169:Caspases / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002478:Cells, Cultured; D002972:Cleft Palate / Q000209:etiology* / Q000378:metabolism*; D003300:Copper / Q000378:metabolism; D017077:Culture Media, Conditioned; D003545:Cysteine / Q000378:metabolism; D004789:Enzyme Activation; D005456:Fluorescent Dyes / Q000378:metabolism; D005494:Folic Acid Deficiency / Q000378:metabolism; D006710:Homocysteine / Q000378:metabolism*; D006801:Humans; D008648:Mesoderm / Q000166:cytology / Q000378:metabolism; D009924:Organ Culture Techniques; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010159:Palate / Q000033:anatomy & histology / Q000502:physiology; D013482:Superoxide Dismutase / Q000378:metabolism; D014212:Tretinoin / Q000378:metabolism
chemical_list: D017077:Culture Media, Conditioned; D005456:Fluorescent Dyes; D006710:Homocysteine; D014212:Tretinoin; D003300:Copper; D002374:Catalase; D013482:Superoxide Dismutase; D020169:Caspases; D003545:Cysteine