Passive immunization with neutralizing antibodies interrupts the mouse mammary tumor virus life cycle.
Mouse mammary tumor virus (MMTV) infects the host via mucosal surfaces and exploits the host immune system for systemic spread and chronic infection. We have tested a neutralizing rat monoclonal antibody specific for the retroviral envelope glycoprotein gp52 for its efficiency in preventing acute and chronic mucosal and systemic infection. The antibody completely inhibits the superantigen response and chronic viral infection following systemic or nasal infection. Surprisingly however, the antibody only partially inhibits the early infection of antigen-presenting cells in the draining lymph node. Despite this initially inefficient protection from infection, superantigen-specific B- and T-cell responses and systemic viral spread are abolished, leading to complete clearance of the retroviral infection and hence interruption of the viral life cycle. In conclusion, systemic neutralizing monoclonal antibodies can provide an efficient protection against chronic retroviral amplification and persistence.
Mpandi
M
M
Swiss Institute for Experimental Cancer Research. Institute of Biochemistry, University of Lausanne, Switzerland.
Otten
L A
LA
Lavanchy
C
C
Acha-Orbea
H
H
Finke
D
D
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage; D000914:Antibodies, Viral / Q000008:administration & dosage; D000957:Antigens, Viral, Tumor / Q000235:genetics / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology / Q000473:pathology; D001483:Base Sequence; D002454:Cell Differentiation; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D018928:Immunity, Mucosal; D007116:Immunization, Passive; D008325:Mammary Neoplasms, Experimental / Q000276:immunology / Q000517:prevention & control; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics / Q000254:growth & development* / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D051381:Rats; D011917:Rats, Inbred Lew; D012192:Retroviridae Infections / Q000276:immunology / Q000517:prevention & control; D014412:Tumor Virus Infections / Q000276:immunology / Q000517:prevention & control
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000957:Antigens, Viral, Tumor; D004279:DNA, Viral; C040612:glycoprotein 52 antigen, Mouse mammary tumor virus