Effect of the aqueous extract of Chrysanthellum indicum on calcium mobilization and activation of rat portal vein.
The effects of the aqueous extract of Chrysanthellum indicum (CI) on calcium activation and mobilization were studied using the rat portal vein. The extract caused a concentration-dependent contraction of the portal vein. KCl (80 mM), norepinephrine (NA, 10(-6)M) and CI (4 mg/ml) evoked sustained contraction of the portal vein. In Ca-free medium (with EGTA) the contractions evoked by these agents were reduced significantly. The times-to-peak of KCl, NA and CI were similar -in normal PSS, but in Ca-free medium the times-to-peak for KCl and CI were greatly increased. The contractions induced by CI were not inhibited by chlorpropamide and prazosin, but were blocked by verapamil. The data obtained suggest that the aqueous extract of CI utilizes extracellular calcium pools to bring about contractile response and this effect might be mediated through the activation of potential-sensitive channels.
Amos
S
S
Department of Pharmacology and Toxicology, National Institute for Pharmaceutical Research and Development, Garki, Abuja, Nigeria. sa7h@virginia.edu
Binda
L
L
Adamu
M
M
Vongtau
H O
HO
Abbah
J
J
Omogbai
E K I
EK
Akah
P A
PA
Bukar
B B
BB
Wambebe
C
C
Gamaniel
K
K
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002747:Chlorpropamide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D008297:Male; D016488:Medicine, African Traditional; D009119:Muscle Contraction / Q000187:drug effects; D009549:Nigeria; D009638:Norepinephrine / Q000494:pharmacology; D010936:Plant Extracts / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000494:pharmacology*; D010946:Plants, Medicinal; D011169:Portal Vein / Q000187:drug effects; D011189:Potassium Chloride / Q000494:pharmacology; D011224:Prazosin / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D010936:Plant Extracts; D011189:Potassium Chloride; D002118:Calcium; D002747:Chlorpropamide; D009638:Norepinephrine; D011224:Prazosin