[Investigation of the effect of Na-butyrate on TNBS induced rat colitis].
OBJECTIVE
To observe the effect of Na-butyrate on trinitrobenzene sulfonic acid (TNBS) induced rat colitis and discuss its mechanism.


METHODS
0.85 ml of 50% ethanol enema containing 30 mg TNBS was instillated into rat colon to produce distal colitis. On the 5th day after TNBS enema, the rats were divided into 3 groups and were given respectively 100 mmol/L Na-butyrate, 100 mg/kg SASP or 0.9% saline enema daily. On the 19th day, macroscopical, histological injury scores of colonic mucosa and tissue myeloperoxidase (MPO) activity were evaluated, and the mitotic index in Na-butyrate group was compared with that in SASP group.


RESULTS
Diarrhea was apparently improved, weight gain was not apparently different, the scores of macroscopical and histological injury and MPO activity decreased in the SASP group and the Na-butyrate group, compared with the control (P < 0.05). However, there was not obvious difference between the Na-butyrate group and SASP group (P > 0.05). Mitotic index in Na-butyrate group was higher than that in SASP group (P < 0.05).


CONCLUSIONS
Na-butyrate enema can expedite the restoration of the experimental colitis, and its therapeutic effect is as good as that of SASP.
Hu
Renwei
R
Department of Gastroenterology, West China Hospital, Sichuan University, Chengdu 610041, China.
Ouyang
Qin
Q
Wang
Hao
H
D000285:Administration, Rectal; D000818:Animals; D002087:Butyrates / Q000494:pharmacology* / Q000627:therapeutic use; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D005260:Female; D051381:Rats; D017207:Rats, Sprague-Dawley; D014302:Trinitrobenzenesulfonic Acid
D002087:Butyrates; D014302:Trinitrobenzenesulfonic Acid