Inflammatory mediators expressed in human islets of Langerhans: implications for islet transplantation.
Expression of immune modulating mediators in human Islets of Langerhans could have important implications for development of autoimmunity in type 1 diabetes and influence the outcome of clinical islet transplantation. Islets obtained from five donors were analyzed at various times after isolation using cDNA array technology. The Atlas Human Cytokine/Receptor and Hematology/Immunology nylon membranes representing 268 genes and 406, respectively, were used and the relative expression of each gene analyzed. Of the 51 gene products identified, high mRNA expression of MCP-1, MIF, VEGF, and thymosin beta-10 was detected in all islet samples. IL-8, IL-1-beta, IL-5R, and INF-gamma antagonist were expressed in islets cultured for 2 days. IL-2R was expressed in islets cultured for more than 6 days. In conclusion, several inflammatory mediators were expressed in isolated islets, particularly at an early stage after isolation, indicating that a few days of culture could be beneficial for the outcome of islet transplantation.
Johansson
Ulrika
U
Department of Clinical Immunology, The Rudbeck Laboratory, Uppsala University C11, Sweden. Ulrika.Johansson@klinimm.uu.se
Olsson
Annika
A
Gabrielsson
Susanne
S
Nilsson
Bo
B
Korsgren
Olle
O
D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D020869:Gene Expression Profiling; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000276:immunology* / Q000378:metabolism; D016381:Islets of Langerhans Transplantation; D020411:Oligonucleotide Array Sequence Analysis; D018121:Receptors, Cytokine / Q000096:biosynthesis / Q000235:genetics
D016207:Cytokines; D018836:Inflammation Mediators; D007328:Insulin; D018121:Receptors, Cytokine