Triplex formation using ODN conjugates with polycation comb-type copolymer.
Polycation comb-type copolymer that is composed of polylysine backbone and dextran side chains (PLL-g-Dex) has previously been shown to stabilize duplex and triplex DNAs quite effectively. In this study, we have conjugated PLL-g-Dex with oligonucleotides (ODN) aiming to increase the triplex stabilizing efficiency of the copolymer. Here we have demonstrated that the copolymer-TFO conjugates selectively stabilize triplex DNA. Also its potential to form triplex DNA was found to be greater than PLL-g-Dex/ODN mixture.
Ueda
M
M
Department of Biomolecular Engineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama, Kanagawa 226-8501, Japan.
Saito
M
M
Ishihara
T
T
Akaike
T
T
Maruyama
A
A
D001483:Base Sequence; D015245:Deoxyribonuclease BamHI; D015252:Deoxyribonucleases, Type II Site-Specific; D003911:Dextrans / Q000737:chemistry; D004355:Drug Stability; D066298:In Vitro Techniques; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D011073:Polyamines / Q000737:chemistry; D000071228:Polyelectrolytes; D011107:Polylysine / Q000031:analogs & derivatives* / Q000737:chemistry
D015245:Deoxyribonuclease BamHI; D015252:Deoxyribonucleases, Type II Site-Specific; D003911:Dextrans; D009838:Oligodeoxyribonucleotides; D011073:Polyamines; D000071228:Polyelectrolytes; D011107:Polylysine; C113090:poly(lysine)-graft-dextran; C047020:endodeoxyribonuclease ScaI