Interaction of proteins with weak amphoteric charged membrane surfaces: effect of pH.
Weak amphoteric charged membranes were prepared by the graft copolymerization of poly(ethylene glycol) (PEG) derivatives with pendant ionizable groups onto polyethylene (PE) porous membranes. Two types of weak amphoteric charged membranes and two types of weak single charged membranes were prepared. The pH dependence of the protein (fluorescein isothiocyanate-labeled bovine serum albumin, FITC-BSA) adsorption onto the membranes was investigated by fluorescence spectroscopy. The interfacial charge properties of the membranes and protein were also characterized at different pH values by streaming potential and electrophoretic light scattering (ELS) measurements, respectively. The adsorbed amount onto each ionic PEG chain grafted membrane showed a uniform maximum value near the isoelectric point (IEP) of the protein (pH 4.1). On both sides of the IEP (pHs 3.3 and 7.2), the adsorption experiments and zeta (zeta) potential measurements were well correlated: the contribution of electrostatic interaction was dominant for the protein adsorption behavior. In the alkaline condition (pH 10.2), the adsorption experiments contradict the zeta potential measurements. It suggested that the conformational change of protein molecule influenced the adsorption behavior. Finally, these results indicated the potential of controlling the protein-ionic PEG chain interaction on the membrane surfaces by the pH adjustment of the outer solution.
Matsumoto
Hidetoshi
H
Department of Organic and Polymeric Materials, and International Research Center of Macromolecular Science, Tokyo Institute of Technology, 2-12-1 Ookayama, Meguro-ku, Tokyo 152-8552, Japan.
Koyama
Yoshiyuki
Y
Tanioka
Akihiko
A
D000327:Adsorption; D000818:Animals; D002417:Cattle; D004586:Electrophoresis / Q000379:methods; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D008567:Membranes, Artificial; D011092:Polyethylene Glycols / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013499:Surface Properties
D008567:Membranes, Artificial; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols