[Annotation of complete genomic sequence of 3p24-p25 478 kb of human DNA].
OBJECTIVE
To annotate the human genome 3p24-p25 478 kb complete sequence.


METHODS
The protein-coding genes in the genomic sequence were identified by using ab initio gene finding, homology-based similarity database searching and all or partial mRNA aligning with genomic sequence, and the content feature of the genomic sequence were analyzed by using EMBOSS package.


RESULTS
Two known genes SLC6A1 and SLC6A11 were identified; as well as the GC content of this genomic sequence was 47% and 3 putative CpG islands were predicted in the genomic sequence, located in 130,685-131,516 bp, 307,090-307,870 bp and 415,585-416,308 bp, respectively.


CONCLUSIONS
The methods, as mentioned above, might be used for annotating the biological information in the genomic sequence, such as gene structure, GC content, CpG island.
Ding
Ke-yue
KY
National Labaratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
Zhang
Yi-lin
YL
Chen
Li-hong
LH
Shen
Yan
Y
D001483:Base Sequence; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial; D002893:Chromosomes, Human, Pair 3; D015894:Genome, Human; D016045:Human Genome Project; D006801:Humans; D008969:Molecular Sequence Data