Stimulation of rat pancreatic exocrine secretion by urocortin and corticotropin releasing factor.
Neural and hormonal mechanisms control pancreatic secretion. The effects of the corticotropin releasing factor (CRF) related neuropeptide urocortin (UCN) on pancreatic exocrine secretion were examined. In anesthetized male rats, pancreatic secretion volume and total protein were assayed. UCN increased pancreatic secretory volume and protein secretion and potentiated cholecytokinin-stimulated protein secretion. Astressin, a non-specific CRF receptor antagonist, inhibited UCN-stimulated protein output while CRF(2) receptor antagonist, antisauvagine-30, was without effect. Atropine, but not subdiaphragmatic vagotomy, inhibited UCN-mediated secretion. In acinar cells, UCN did not stimulate release of amylase nor intracellular cAMP. UCN is a pancreatic exocrine secreatagogue with effects mediated through cholinergic intrapancreatic neurons.
Guzman
Eduardo A
EA
Department of Surgery, University of Michigan Medical Center, Ann Arbor, MI 48109-0331, USA.
Zhang
Weizhen
W
Lin
Theodore R
TR
Mulholland
Michael W
MW
D000818:Animals; D001285:Atropine / Q000494:pharmacology; D003346:Corticotropin-Releasing Hormone / Q000494:pharmacology*; D000242:Cyclic AMP / Q000378:metabolism; D006801:Humans; D008297:Male; D010179:Pancreas / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018019:Receptors, Corticotropin-Releasing Hormone / Q000378:metabolism; D013997:Time Factors; D054832:Urocortins; D014628:Vagotomy; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
D018019:Receptors, Corticotropin-Releasing Hormone; D054832:Urocortins; D014660:Vasoactive Intestinal Peptide; D001285:Atropine; D003346:Corticotropin-Releasing Hormone; D000242:Cyclic AMP