UniFrag and GenomePrimer: selection of primers for genome-wide production of unique amplicons.
The complementary programs UniFrag and GenomePrimer were developed to provide a reliable high-throughput method to select the most unique regions within genomic DNA sequence(s) and design primers therein, involving minimal user intervention and maximum flexibility.
van Hijum
Sacha A F T
SA
Molecular Genetics, University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute, PO Box 14, 9750 AA Haren, The Netherlands.
de Jong
Anne
A
Buist
Girbe
G
Kok
Jan
J
Kuipers
Oscar P
OP
D000465:Algorithms; D017931:DNA Primers / Q000138:chemical synthesis / Q000737:chemistry* / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D016415:Sequence Alignment / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*; D012984:Software
D017931:DNA Primers