[An observation of taurine transport alterations in calcification of myocardial cells in vitro].
OBJECTIVE
To observe the alterations of taurine transport, taurine transporter (TAUT) and cysteine sulfinate decarboxylase (CSD) mRNA in the calcification of myocardial cells in vitro.


METHODS
3H-taurine measured the amount of taurine uptake. TAUT and CSD mRNA consents were measured using competitive quantitative RT-PCR in cultured and calcified myocardial cells.


RESULTS
In calcification of myocardial cells, taurine concentration was decreased by 27% (P < 0.05), taurine uptake was markedly reduced, Vmax reduced by 39% (P < 0.01), there were no statistical significance of Km values between the two groups. TAUT mRNA decreased by 45% (P < 0.01), but CSD mRNA increased by 25% (P < 0.05).


CONCLUSIONS
The data suggest that there were impediment of taurine transport in calcification of myocardial cells, as TAUT mRNA level was decreased, but CSD mRNA concentration was improved.
Shi
Yan-rong
YR
Institute of Cardiovascular Disease Reserch, First Hospital, Peking University, Beijing 100034, China.
Wang
Shu-heng
SH
Bu
Ding-fang
DF
Qi
Yong-fen
YF
Gao
Lin
L
Pang
Yong-zheng
YZ
Tang
Chao-shu
CS
D000818:Animals; D001692:Biological Transport; D002114:Calcinosis / Q000378:metabolism / Q000473:pathology*; D002118:Calcium / Q000378:metabolism; D002262:Carboxy-Lyases / Q000378:metabolism; D002478:Cells, Cultured; D032383:Myocytes, Cardiac / Q000378:metabolism* / Q000473:pathology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D013654:Taurine / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger; D013654:Taurine; D002262:Carboxy-Lyases; C019972:sulfoalanine decarboxylase; D002118:Calcium