Tumor necrosis factor-alpha in low doses preactivates and activates macrophages by increasing their ability to produce reactive oxygen species and oxidize low-density lipoproteins: protective effect of antioxidants.
Tumor necrosis factor-alpha in low doses activated rat peritoneal macrophages and intensified production of reactive oxygen species (zymosan-depended chemiluminescence). Single or 2-fold incubation with tumor necrosis factor-a activated and preactivated human blood macrophages and promoted oxidative modification of low-density lipoproteins (increased their mobility in agarose gel). Antioxidants (potassium phenosan, probucol, and desferal) suppressed oxidative modification of low-density lipoproteins induced by nonactivated, preactivated, and activated macrophages. Our results show that antioxidants hold much promise for the prevention and therapy of atherosclerosis.
Bilenko
M V
MV
V. N. Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow. bil@ibmh.msk.su
Khil'chenko
A V
AV
Shmit'ko
N A
NA
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D006801:Humans; D008077:Lipoproteins, LDL / Q000737:chemistry / Q000378:metabolism*; D008262:Macrophage Activation / Q000502:physiology*; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D010084:Oxidation-Reduction; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism / Q000494:pharmacology*; D015054:Zymosan / Q000378:metabolism
D000975:Antioxidants; D008077:Lipoproteins, LDL; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; D015054:Zymosan