Real-time detection of PCR products for comparative analysis of expressed genes using module-shuffling TaqMan probes (MTPs).
A method for comparative analysis of gene expression was developed. It is based on competitive PCR amplification and real-time detection of PCR products and it uses module-shuffling sequences as "universal TaqMan probes". Namely, cDNA-tagged module-shuffling sequences, which derived from different sources, were amplified in one reaction tube by the same primer set. Two kinds of fluorescent TaqMan probes with different module-shuffling sequences (MTPs) detect their own targets. The method can detect different amounts of expressed genes derived from different sources; accordingly, it was successfully used for comparative analysis of expressed mouse genes.
Uematsu
Chihiro
C
Biosystems Research Department, Central Research Laboratory, Hitachi, Ltd., 1-280, Higashi-koigakubo Kokubunji-shi, Tokyo, 185-8601, Japan.
Makino
Ikuko
I
Okano
Kazunori
K
D001483:Base Sequence; D015342:DNA Probes; D020869:Gene Expression Profiling; D016133:Polymerase Chain Reaction / Q000379:methods*
D015342:DNA Probes