[Study on Aconitum diterpenoid alkaloids from flowers of Aconitum kusnezoffi and its decoction by ESI-MS].
OBJECTIVE
To study the change of chemical compound after decocting the flowers of Aconitum kusnzoffii Reichb (FAK) by analyzing the alkaloids in FAK and the decoction of FAK qualitatively.


METHODS
The alkaloid extracts of FAK and alkaloid mixtures in the decoction of FAK were directly analyzed by electrospray ionization trap tandem mass spectrometry via by pump.


RESULTS
Three novel aconitum alkaloids were found in FAK. In addition, it is found that both diester diterpenoid alkaloids and triester diterpenoid alkaloids were hydrolyzed in the decoction of FAK. The hydrolysis products of the diester-alkaloids are benzoylaconines and aconines; the hydrolysis products of the triester-alkaloids are 3-acetyl-aconines.


CONCLUSIONS
ESI-MS" method is suitable for the analysis of Aconitum alkaloids in the decoction of FAK as well as other Aconitum plants, which offers the advantages of convenience, speed, sensitivity and specificity.
Wang
Yong
Y
New Drug Lab of Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Department of Chemistry, Northeast Normal University, Changchun, China.
Liu
Zhi-qiang
ZQ
Song
Feng-rui
FR
Liu
Shu-ying
SY
D031841:Aconitum / Q000737:chemistry*; D000470:Alkaloids / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D035264:Flowers / Q000737:chemistry*; D006358:Hot Temperature; D006868:Hydrolysis; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
D000470:Alkaloids; D004365:Drugs, Chinese Herbal; C054803:lepenine