Biolistic transfection.
Recent improvements in the biolistic technique and devices have increased its usefulness for transfection of neurons. With these recent advances, both dissociated and slice cultures can be transfected at reasonably high rates. This chapter focuses on the parameters that determine the successful biolistic transfection of neurons in both types of cultures.
Bridgman
Paul C
PC
Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Brown
Michael E
ME
Balan
Irina
I
D000818:Animals; D019470:Biolistics / Q000295:instrumentation / Q000379:methods*; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002478:Cells, Cultured / Q000166:cytology / Q000502:physiology*; D004247:DNA / Q000235:genetics / Q000494:pharmacology; D006801:Humans; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D010316:Particle Size; D014162:Transfection / Q000295:instrumentation / Q000379:methods*
D004247:DNA