[Cloning cDNA encoding for GAGA-like element binding proteins in human Jurkat cells].
OBJECTIVE
To explore GAGA-like element binding protein in human cells.


METHODS
Yeast one-hybrid system was used to screen the GAGA-like element binding proteins in HTLV-1 transformed Jurkat cell cDNA fusion library. Total RNA extracted from Jurkat cells was first labeled by reverse transcription, and was taken as cDNA probe to hybridize with the candidate positive clones.


RESULTS
9 positive clones were obtained, and 6 out of the 9 clones were positively hybridized with the cDNA probe.


CONCLUSIONS
6 candidate clones encoding for GAGA-like element binding proteins were obtained from Jurkat cells for further investigation.
Mo
Zhi-cheng
ZC
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
Lu
Yu
Y
Gao
Chao
C
Wu
Ning-hua
NH
Shen
Yu-fei
YF
D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D018841:HSP90 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D018398:Homeodomain Proteins / Q000235:genetics*; D015368:Human T-lymphotropic virus 1 / Q000235:genetics; D006801:Humans; D019169:Jurkat Cells; D015458:Leukemia, T-Cell / Q000378:metabolism* / Q000473:pathology; D014157:Transcription Factors / Q000235:genetics*; D020798:Two-Hybrid System Techniques
D002352:Carrier Proteins; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D018841:HSP90 Heat-Shock Proteins; D018398:Homeodomain Proteins; D014157:Transcription Factors; C063460:Trl protein, Drosophila