Synthesis of oligodeoxyribonucleotides containing 2'-deoxypseudouridine: inhibition of uracil-DNA glycosylase.
2'-Deoxypseudouridine (1) was synthesized starting from thymidine in a 23% overall yield via a silylated furanoid glycal. Compound 1 was introduced to oligodeoxyribonucleotides (ODNs) by the solid phase phosphoramidite chemistry. The ODNs containing 1 inhibited uracil-DNA glycosylase (UDG) reaction.
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Department of Chemistry, Faculty of Science, Tokyo Metropolitan University.
Nishizima
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D045647:DNA Glycosylases; D004791:Enzyme Inhibitors / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D004926:Escherichia coli / Q000201:enzymology; D008722:Methods; D015394:Molecular Structure; D009699:N-Glycosyl Hydrolases / Q000037:antagonists & inhibitors*; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D011560:Pseudouridine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D051981:Uracil-DNA Glycosidase
D004791:Enzyme Inhibitors; D009838:Oligodeoxyribonucleotides; D011560:Pseudouridine; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase