title: Functional expression and production of human H-ferritin in Pichia pastoris.
abstract: Human heavy chain ferritin (H-ferritin) was cloned from human heart cDNA library and expressed in Pichia pastoris. The H-ferritin transformant was cultivated by fed-batch and the cell mass reached about 52 g cell dry wt l(-1) after 150 h. In atomic absorption spectrometry analysis, intracellular content of iron in H-ferritin transformant was measured to 3038 +/- 72 microg g(-1) which was 9.6-fold more than that of control strain.
authors.affiliation: College of Life Sciences, Kyung Hee University, Suwon 449-701, Korea.
authors.forename: Jung-Lim
authors.initials: JL
authors.lastname: Lee
authors.forename: Hyung-Seok
authors.initials: HS
authors.lastname: Song
authors.forename: Hyun-Joong
authors.initials: HJ
authors.lastname: Kim
authors.forename: Jong-Hwan
authors.initials: JH
authors.lastname: Park
authors.forename: Dae-Kyun
authors.initials: DK
authors.lastname: Chung
authors.forename: Cheon-Seok
authors.initials: CS
authors.lastname: Park
authors.forename: Dooil
authors.initials: D
authors.lastname: Jeoung
authors.forename: Hae-Yeong
authors.initials: HY
authors.lastname: Kim
mesh_terms: D003001:Cloning, Molecular; D005293:Ferritins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D015966:Gene Expression Regulation, Fungal; D006801:Humans; D007501:Iron / Q000032:analysis; D010843:Pichia / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D011786:Quality Control; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; D005293:Ferritins; D007501:Iron