title: Synthesis and properties of oligonucleotides containing novel 2',4'-BNA analogues (2',4'-BNACOC).
abstract: Preorganization of the nucleoside into proper conformation is one of the most promising approaches to develop the oligonucleotides strongly interacting with nucleic acid targets in a sequence-specific manner. We designed and synthesized the 2',4'-BNACOC monomer as a novel bridged nucleic acid analogue possessing a fixed N-type sugar conformation, and also successfully achieved its incorporation into oligonucleotides. The 2',4'-BNACOC modified oligonucleotides were found to have selective and strong binding-affinity for complementary RNA rather than DNA, and to show an excellent nuclease resistance ability.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Hari
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Osaki
authors.forename: Ken
authors.initials: K
authors.lastname: Eguchi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Obika
authors.forename: Takeshi
authors.initials: T
authors.lastname: Imanishi
mesh_terms: D001483:Base Sequence; D009696:Nucleic Acids / Q000737:chemistry*; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*
chemical_list: D009696:Nucleic Acids; D009841:Oligonucleotides