Improved molecular detection of dengue virus serotype 1 variants.
The dengue virus molecular typing method described by Lanciotti and coworkers (R. S. Lanciotti, C. H. Calisher, D. J. Gubler, G. J. Chang, and A. Vance-Vorndam, J. Clin. Microbiol. 30:545-551, 1992) is used worldwide for diagnosis and surveillance. However, it failed to detect DENV-1 variants in Cambodia due to a point mutation. We describe an improvement of the method that allows the detection of additional DENV-1 strains, including potential variants.
Reynes
Jean-Marc
JM
Institut Pasteur du Cambodge. National Dengue Control Programme, Ministry of Health. World Health Organization, Phnom Penh, Cambodia. jmreynes@pasteur-kh.org
Ong
Sivuth
S
Mey
Channa
C
Ngan
Chantha
C
Hoyer
Stefan
S
Sall
Amadou A
AA
D001483:Base Sequence; D003715:Dengue / Q000175:diagnosis / Q000517:prevention & control; D003716:Dengue Virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D005784:Gene Amplification; D005814:Genes, Viral; D005843:Geography; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction