[Establishment of drug-resistance cell line of human glioma mediated by MGMT].
OBJECTIVE
To establish a drug-resistance cell line of human glioma mediated by MGMT.


METHODS
Simulated the clinical usage of BCNU to establish a BCNU-resistant human glioma subline by cyclic exposing the U251 parent cells to a constant concentration of BCNU. The resistance index and the expression of MGMT mRNA of U251/BCNU were detected and compared the difference of in vitro proliferation between U251 and U251/BCNU.


RESULTS
A subline--U251/BCNU was successfully established in about 4-month culture, which had a stable resistance to BCNU. U251/BCNU cells showed 17-fold higher resistance to BCNU than did U251 cells by MTT assay, while U251/BCNU cells expressed MGMT mRNA. The doubling time of U251 and U251/BCNU had no statistical difference.


CONCLUSIONS
A drug-resistance cell line of human glioma mediated by MGMT is established, which could provide experimental basis for further studies on the resistance mechanism and reversal methods of glioma chemotherapy.
Jin
Peng
P
Department of Neurosurgery, Second Hospital of Shandong University, Jinan 250033, China.
Zhang
Qing-lin
QL
Liu
Fu-sheng
FS
Wang
Bao-an
BA
Wei
Lin
L
Wang
Cheng-wei
CW
Kong
Jian-xin
JX
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000096:biosynthesis; D001932:Brain Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D015254:DNA Modification Methylases / Q000096:biosynthesis / Q000235:genetics; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005910:Glioma / Q000473:pathology*; D006801:Humans; D019853:O(6)-Methylguanine-DNA Methyltransferase / Q000378:metabolism / Q000502:physiology*
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D015254:DNA Modification Methylases; D019853:O(6)-Methylguanine-DNA Methyltransferase