title: [Specific determination of flaviviruses by molecular hybridization with synthetic deoxyoligonucleotide probes].
abstract: Molecular probes were designed for the purpose of specific determination of flavioviruses transmitted by the ticks of tick-borne encephalitis (TBE) and Omsk hemorrhagic fever (OHV) as well as by mosquitoes of Japanese encephalitis (JE), North Nile (NN), Murrey Valley encephalitis (MVE), Saint-Lois encephalitis (SLE), dengue 1-4 and of yellow fever (YF). The probes are synthetic deoxyoligonucleotides with the 18-20 long basis and complementary for the RNA fragments defined by computer analysis. The thus obtained probes, which specifically hybridize themselves with the sets of the TBE virus or of the OHV virus and do not hybridize themselves with other TBE viruses' sets. Group-specific probes for YE and dengue viruses as well as virus-specific probes, which are able to detect each of the above viruses without any cross effects, were suggested for indexing and identifying the flaviviruses transmitted by mosquitoes.
authors.forename: V I
authors.initials: VI
authors.lastname: Zlobin
authors.forename: V V
authors.initials: VV
authors.lastname: Pogodina
authors.forename: D A
authors.initials: DA
authors.lastname: Drokin
authors.forename: M Kh
authors.initials: MKh
authors.lastname: Gazo
authors.forename: N G
authors.initials: NG
authors.lastname: Bochkova
authors.forename: Iu P
authors.initials: IuP
authors.lastname: Dzhioev
authors.forename: T V
authors.initials: TV
authors.lastname: Demina
authors.forename: S I
authors.initials: SI
authors.lastname: Belikov
authors.forename: I V
authors.initials: IV
authors.lastname: Kozlova
authors.forename: M M
authors.initials: MM
authors.lastname: Verkhozina
mesh_terms: D004669:Encephalitis Viruses, Tick-Borne / Q000235:genetics / Q000302:isolation & purification; D005416:Flavivirus / Q000235:genetics* / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000138:chemical synthesis / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014759:Viral Envelope Proteins / Q000235:genetics
chemical_list: D015345:Oligonucleotide Probes; D012367:RNA, Viral; D014759:Viral Envelope Proteins; C065696:glycoprotein E, Flavivirus