A new strategy to determine the target DNA sequence of a short peptide.
The design of molecules that target desired DNA sequences has been one of the major challenges in the field of molecular recognition. We report here a general strategy for defining the sequence-preference of DNA-binding short peptide by using its heterodimer. Our method successfully identified specific sequences of short peptides derived from native DNA-binding proteins. The usefulness of this approach has been demonstrated by identifying preferred DNA targets for a peptide composed only of D-amino acids.
Sato
Shin-ichi
S
PRESTO, JST, Kyoto University, Uji, Kyoto 611-0011, Japan.
Honjyo
Hirokazu
H
Tsuji
Shinji
S
Hagihara
Masaki
M
Makino
Keisuke
K
Morii
Takashi
T
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D024202:Electrophoretic Mobility Shift Assay; D010455:Peptides / Q000737:chemistry*
D010455:Peptides; D004247:DNA