title: Preparation and enzymatic recognition of guanine lesions induced by nitrogen oxides.
abstract: Xanthine (Xan) and oxanine (Oxa) are the major deamination products of guanine formed by the treatment with nitrogen oxides (e.g., NO and HNO2). In this study, 2'-deoxyribonucleoside 5'-triphosphates of Xan and Oxa were prepared by the NaNO2 treatment of dGTP. These modified nucleotides were incorporated into oligonucleotides by DNA polymerase reactions. The repair activities of various DNA N-glycosylases for Xan and Oxa were examined using these substrates.
authors.affiliation: Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima 739-8526, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Masaoka
authors.forename: H
authors.initials: H
authors.lastname: Terato
authors.forename: A
authors.initials: A
authors.lastname: Honsho
authors.forename: Y
authors.initials: Y
authors.lastname: Ohyama
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.forename: M
authors.initials: M
authors.lastname: Yamada
authors.forename: K
authors.initials: K
authors.lastname: Makino
authors.forename: H
authors.initials: H
authors.lastname: Ide
mesh_terms: D004249:DNA Damage; D045647:DNA Glycosylases; D004260:DNA Repair; D003854:Deoxyribonucleotides / Q000378:metabolism; D006147:Guanine / Q000378:metabolism*; D066298:In Vitro Techniques; D009699:N-Glycosyl Hydrolases / Q000378:metabolism; D009569:Nitric Oxide / Q000633:toxicity*; D009585:Nitrogen Dioxide / Q000633:toxicity*; D009711:Nucleotides / Q000378:metabolism; D011684:Purine Nucleosides / Q000378:metabolism; D011758:Pyrroles / Q000378:metabolism; D019820:Xanthine / Q000378:metabolism
chemical_list: C110411:1-(2'-deoxyribofuranosyl)-3-nitropyrrole triphosphate; D003854:Deoxyribonucleotides; D009711:Nucleotides; D011684:Purine Nucleosides; D011758:Pyrroles; C468766:oxanine; D019820:Xanthine; D009569:Nitric Oxide; D006147:Guanine; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D009585:Nitrogen Dioxide