title: Mutational analysis of cell wall biosynthesis in Mycobacterium avium.
abstract: The cell wall of the environmental pathogen Mycobacterium avium is important to its virulence and intrinsic antimicrobial resistance. To identify genes involved in cell wall biosynthesis, "transposome" insertion libraries were screened for mutants with altered colony morphology on medium containing the lipoprotein stain Congo red. Nineteen such mutants were isolated and mapped, including 10 with insertions in a functional island of cell wall biosynthetic genes that spans approximately 40 kb of the M. avium genome.
authors.affiliation: Seattle Biomedical Research Institute, Seattle, Washington 98109, USA.
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Laurent
authors.forename: Kirsten
authors.initials: K
authors.lastname: Hauge
authors.forename: Kellie
authors.initials: K
authors.lastname: Burnside
authors.forename: Gerard
authors.initials: G
authors.lastname: Cangelosi
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002473:Cell Wall / Q000235:genetics / Q000378:metabolism*; D003224:Congo Red / Q000378:metabolism; D004251:DNA Transposable Elements; D015723:Gene Library; D006017:Glycolipids / Q000378:metabolism; D006020:Glycopeptides / Q000378:metabolism; D016254:Mutagenesis, Insertional; D009154:Mutation; D009162:Mycobacterium avium / Q000235:genetics* / Q000254:growth & development
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D006017:Glycolipids; D006020:Glycopeptides; D003224:Congo Red