[On preparation the monoclonal antibody against synthetic antigen of cyclosporine A].
OBJECTIVE
To study the methods for preparing the monoclonal antibody against synthetic antigen of cyclosporine A.


METHODS
These included using photochemical reaction to couple cyclosporine A with poly-L-lysine as synthetic antigen, immunizing BALB/c mice, fusing spleen cells and SP2/0 cells. Then hybridoma cells were repeatedly screened and subcloned. The monoclonal antibodies were identified by indirect ELISA.


RESULTS
Four hybridoma cells in secreting anti-cyclosporine A monoclonal antibodies were obtained. Only two showed high titer and some specificity.


CONCLUSIONS
The methods of antigen synthesis and immunization were practicable. But the methods of antibody preparation and preservation need to be improved.
Chen
Mina
M
Department of Infectious Disease, West China Hospital, Sichuan University, Chengdu 610041, China.
Fan
Xinjian
X
Wei
Dapeng
D
Wu
Qiongying
Q
Feng
Ping
P
Lü
Xiaoju
X
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D016572:Cyclosporine / Q000276:immunology*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D011107:Polylysine / Q000276:immunology*; D013154:Spleen / Q000166:cytology
D000911:Antibodies, Monoclonal; D011107:Polylysine; D016572:Cyclosporine