Aberrant T cell activation and heightened apoptotic turnover in end-stage renal failure patients: a comparative evaluation between non-dialysis, haemodialysis, and peritoneal dialysis.
Patients in end-stage renal disease (ESRD) have a high incidence of bacterial and viral infections. Fifteen non-dialysed (ND), 15 haemodialysed (HD), 15 patients with peritoneal dialysis (PD), and 15 healthy controls were included. T cell proliferation was measured by [3H]thymidine uptake. Apoptosis and cell phenotype were determined by FACS. sTNF-R1, sCD95, interleukin-1beta-converting enzyme (sICE), and interleukin (IL)-10 were measured by ELISA. PHA and CD3-driven T cell proliferation were significantly decreased in ESRD patients. CD3(+), CD19(+) B cells, and percentage of CD4(+) T cells were significantly reduced. Percent memory T cells (CD45RO(+)) and cells undergoing apoptosis (CD95(+)/Annexin V+) were significantly increased in ESRF. Moreover, sCD95, sTNFRI, and ICE were significantly increased. Serum level of IL-10, a Th2 cytokine, was enhanced. These findings strongly suggest that in ESRD patients Th1 T cells are selectively susceptible to undergo apoptosis. This observation provides an additional pathophysiological concept in the genesis of Th2 dominance.
Moser
Bernhard
B
Department of Surgery, General Hospital of Vienna, Austria.
Roth
Georg
G
Brunner
Markus
M
Lilaj
Tatjana
T
Deicher
Robert
R
Wolner
Ernst
E
Kovarik
Josef
J
Boltz-Nitulescu
George
G
Vychytil
Andreas
A
Ankersmit
Hendrik Jan
HJ
D000368:Aged; D017209:Apoptosis; D020170:Caspase 1 / Q000097:blood; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D016753:Interleukin-10 / Q000097:blood; D007676:Kidney Failure, Chronic / Q000276:immunology* / Q000473:pathology / Q000628:therapy*; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D010531:Peritoneal Dialysis, Continuous Ambulatory; D018124:Receptors, Tumor Necrosis Factor / Q000097:blood / Q000378:metabolism; D006435:Renal Dialysis; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*; D019014:fas Receptor / Q000097:blood / Q000378:metabolism
D018124:Receptors, Tumor Necrosis Factor; D019014:fas Receptor; D016753:Interleukin-10; D017493:Leukocyte Common Antigens; D020170:Caspase 1