title: Macrophage inflammatory protein-related protein-2, a novel CC chemokine, can regulate preadipocyte migration and adipocyte differentiation.
abstract: Adipocytes not only store energy, but also secrete biologically active molecules called adipocytokines, which play a pivotal role in adipocyte-related pathological processes such as diabetes and cardiovascular disease. Recent studies have shown that preadipocyte/adipocyte expresses chemokines (e.g. monocyte chemoattractant protein-1, macrophage inflammatory protein-1 alpha) which alter adipocyte function, indicating the involvement of chemokines in adipocyte-related pathologies. The current study investigated the potential of macrophage inflammatory protein-related protein-2 (MRP-2), a novel CC chemokine, to modulate preadipocyte trafficking and adipocyte differentiation. MRP-2 and its receptors were highly expressed in preadipocytes and differentiated adipocytes as well as in the mouse fat pad. Chemotaxis assays revealed that MRP-2 was a specific chemotactic regulator in preadipocyte migration. The levels of MRP-2 expression in adipose tissue were enhanced in obese mice compared to lean mice. MRP-2 secretion by preadipocytes was suppressed during differentiation. MRP-2 suppressed the expression of adipocyte differentiation markers such as adipocyte fatty acid-binding protein and glycerol-3 phosphate dehydrogenase. Taken together, our data suggest that MRP-2 plays a role in the regulation of preadipocyte migration and adipocyte differentiation during adipose tissue development. MRP-2 may be another adipocytokine, which can be involved in the adipocyte-related pathological process.
authors.affiliation: Department of Food Science and Nutrition, University of Ulsan, Mugeo-dong, Nam-ku, Ulsan 680-749, South Korea.
authors.forename: Chu-Sook
authors.initials: CS
authors.lastname: Kim
authors.forename: Teruo
authors.initials: T
authors.lastname: Kawada
authors.forename: Hoon
authors.initials: H
authors.lastname: Yoo
authors.forename: Byung-Se
authors.initials: BS
authors.lastname: Kwon
authors.forename: Rina
authors.initials: R
authors.lastname: Yu
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D019742:Chemokines, CC / Q000032:analysis / Q000235:genetics / Q000502:physiology; D002633:Chemotaxis; D005260:Female; D019402:Macrophage Inflammatory Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009765:Obesity / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis; D015854:Up-Regulation
chemical_list: C095218:Ccl9 protein, mouse; D019742:Chemokines, CC; D019402:Macrophage Inflammatory Proteins; D012333:RNA, Messenger