Adsorption and isotope effects by ion exchange with 1-aza-12-crown-4 bonded Merrifield peptide resin.
Magnesium isotope effects were investigated by chemical ion exchange with synthesized 1-aza-12-crown-4 bonded Merrifield peptide resin using elution chromatography. The capacity of azacrown ion exchanger was 0.89 meq/g dry resin. The heavier isotopes of magnesium were enriched in the resin phase, while the lighter isotopes were enriched in the solution phase. The hydration effect is less than the complexation and isotope mass effects. The single stage separation factor was determined according to the method of Glueckauf from the elution curve and isotopic assays. The separation factors of 24Mg(2+)-25Mg(2+), 24Mg(2+)-26Mg(2+), and 25Mg(2+)-26Mg(2+) were 1.012, 1.023, and 1.011, respectively.
Kim
Dong Won
DW
Department of Chemistry, Physical Chemistry Laboratory, College of Natural Sciences, Chungbuk National University, Cheongju, 361-763, South Korea. chem131@trut.chungbuk.ac.kr
Jeon
Byeong Kwang
BK
Kang
Byung Moo
BM
Lee
Nam-Soo
NS
Ryu
Hai-il
HI
Lee
Yong-il
YI
D002852:Chromatography, Ion Exchange; D043844:Crown Ethers / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004988:Ethers, Cyclic / Q000737:chemistry; D007554:Isotopes / Q000737:chemistry; D007700:Kinetics; D008274:Magnesium / Q000737:chemistry*; D008956:Models, Chemical; D010455:Peptides / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*
C480580:1-aza-12-crown-4; D043844:Crown Ethers; D004988:Ethers, Cyclic; D007554:Isotopes; C095039:Merrifield resin; D010455:Peptides; D011137:Polystyrenes; D008274:Magnesium