[Establishment and application of a RT-PCR system for quantifying mRNA of 4 hsp genes in human cells].
OBJECTIVE
To establish a RT-PCR system for detecting mRNA expression level of 4 hsp genes in human cells.


METHODS
RT-PCR system was established with gene cloning, gene recombination, in vitro transcription and RT-PCR techniques; The detection of expression level of 4 hsp genes in SW13 cell was carried out with this system.


RESULTS
In SW13 cell, hsp70 and hsp90 alpha were typical heat shock induced genes, while hsp60 and hsp90 beta were efficiently expressed and further induced by heat-shock to various extent.


CONCLUSIONS
In our hands novel RT-PCR system can be used to detect mRNA expression level of 4 human hsp genes.
Li
Hong-fan
HF
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
Liu
Xing-rong
XR
Heng
Feng-yan
FY
Wu
Ning-hua
NH
Shen
Yu-fei
YF
D018834:Chaperonin 60 / Q000096:biosynthesis / Q000235:genetics*; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics*; D018841:HSP90 Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics*; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D015458:Leukemia, T-Cell / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D018834:Chaperonin 60; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D012333:RNA, Messenger