Application of L-cystein derivative to DNA microarray.
S-carboxymethyl-L-cystein derivatives of nucleic acid bases were prepared as DNA chip probe. These compounds in vitro have been found to form stable complex with oligo-DNA and RNA. This paper deals with preparing new DNA chip using L-cystein derivative synthetic nucleotides as probe and immobilized it to quartz plate by photosensitive PVA. Then the chip exposed with FITC labeled target DNA was observed by confocal fluorescence microscope.
Nakauchi
Gen
G
Department of Material and Life Science, Graduate School of Engineering, Osaka University, Yamadaoka 2-1, Suita, Osaka 565-0871, Japan.
Inaki
Yoshiaki
Y
Kitaoka
Shiho
S
Yokoyama
Chieko
C
Tanabe
Tadashi
T
D003545:Cysteine / Q000737:chemistry*; D018613:Microscopy, Confocal; D015335:Molecular Probes; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis
D015335:Molecular Probes; D003545:Cysteine