Anti-obesity effect of Dioscorea nipponica Makino with lipase-inhibitory activity in rodents.
In the process of screening for pancreatic lipase inhibitors, which could be used as an anti-obesity measure, the methanol extract of Dioscorea nipponica Makino powder (DP) appeared to have potent inhibitory activity against porcine pancreatic lipase with an IC50 value of 5-10 microg/ml, where the enzyme activity was assayed by using 4-methylumbelliferyl oleate as a substrate. Further purification of active components present in the herb generated dioscin that belongs to the saponin family. Dioscin and its aglycone, diosgenin, both suppressed the time-dependent increase of blood triacylglycerol level when orally injected with corn oil to mice, suggesting their inhibitory potential against fat absorption. Sprague-Dawley rats fed on a high-fat diet containing 5% Dioscorea nipponica Makino and 40% beef tallow gained significantly less body weight and adipose tissue than control animals fed on a high-fat diet alone during an 8-week experimental period (P<0.05).
Kwon
Chong-Suk
CS
Department of Food Science and Nutrition, Andong National University, S. Korea.
Sohn
Ho Yong
HY
Kim
Sung Hee
SH
Kim
Ji Hyun
JH
Son
Kun Ho
KH
Lee
Jeong Soon
JS
Lim
Jin Kyu
JK
Kim
Jong-Sang
JS
D000273:Adipose Tissue / Q000187:drug effects; D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001835:Body Weight / Q000187:drug effects; D004032:Diet; D004041:Dietary Fats / Q000008:administration & dosage / Q000009:adverse effects; D027742:Dioscorea / Q000737:chemistry*; D004144:Diosgenin / Q000031:analogs & derivatives* / Q000494:pharmacology; D004655:Emulsions / Q000008:administration & dosage; D005243:Feces / Q000737:chemistry; D008049:Lipase / Q000037:antagonists & inhibitors*; D008297:Male; D051379:Mice; D009765:Obesity / Q000097:blood / Q000188:drug therapy / Q000517:prevention & control*; D009929:Organ Size / Q000187:drug effects; D010179:Pancreas / Q000201:enzymology; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D014280:Triglycerides / Q000097:blood
D019440:Anti-Obesity Agents; D004041:Dietary Fats; D004655:Emulsions; D010936:Plant Extracts; D014280:Triglycerides; C019357:dioscin; D008049:Lipase; D004144:Diosgenin