DNA dynamics in a microchannel.
An extended Brownian dynamics simulation method is used to characterize the dynamics of long DNA molecules flowing in microchannels. The relaxation time increases due to confinement in agreement with scaling predictions. During flow the molecules migrate toward the channel center line, and thereby segregate according to molecular weight. Capturing these effects requires the detailed incorporation of solvent flow in the simulation method, demonstrating the importance of hydrodynamic effects in the dynamics of confined macromolecules.
Jendrejack
Richard M
RM
Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Dimalanta
Eileen T
ET
Schwartz
David C
DC
Graham
Michael D
MD
de Pablo
Juan J
JJ
D003198:Computer Simulation; D004247:DNA / Q000737:chemistry*; D007700:Kinetics; D008956:Models, Chemical; D013269:Stochastic Processes; D014783:Viscosity
D004247:DNA