Construction of YAC/BAC contig map for the BTA 6q21 region containing a locus for bovine chondrodysplastic dwarfism.
To characterize the bovine chromosome 6q21 for bovine chondrodysplastic dwarfism (BCD), we developed 48 new microsatellite markers from yeast artificial chromosome (YAC) and bacterial artificial chromosome (BAC) clones using a modified magnetic bead capture method. These new markers were used to construct a high-resolution physical map of the region with a total of 85 loci. The physical map will be a powerful tool for successful positional cloning experiments.
Takeda
Haruko
H
Shirakawa Institute of Animal Genetics, Odakura, Nishigo, Fukushima, Japan. haruko@coccoa.ocn.ne.jp
Sugimoto
Yoshikazu
Y
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000235:genetics*; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics*; D033481:Chromosomes, Mammalian / Q000235:genetics*; D020451:Contig Mapping; D005097:Exostoses, Multiple Hereditary / Q000235:genetics* / Q000662:veterinary*; D005819:Genetic Markers; D018895:Microsatellite Repeats / Q000235:genetics; D016324:Sequence Tagged Sites
D005819:Genetic Markers