Syntaphilin binds to dynamin-1 and inhibits dynamin-dependent endocytosis.
Syntaphilin is a brain-specific syntaxin-binding partner first characterized as an inhibitor of SNARE complex formation and neurotransmitter release. Here we show that syntaphilin also binds to dynamin-1 and through this interaction inhibits dynamin-mediated endocytosis. Immunoprecipitation studies from cross-linked rat synaptosomes demonstrate that an endogenous syntaphilin-dynamin-1 complex exists independently of dynamin-1 binding to amphiphysin. Functionally, syntaphilin expression inhibits transferrin internalization in COS-7 cells. These data reveal that syntaphilin is an inhibitor of both SNARE-based fusion and dynamin-mediated endocytosis.
Das
Sunit
S
Synaptic Function Unit, NINDS, National Institutes of Health, Bethesda, Maryland 20892-4154, USA.
Gerwin
Claudia
C
Sheng
Zu-Hang
ZH
D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism; D019556:COS Cells; D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D034284:Dynamin I / Q000378:metabolism*; D004705:Endocytosis; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D013058:Mass Spectrometry; D008565:Membrane Proteins; D008957:Models, Genetic; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D011233:Precipitin Tests; D011485:Protein Binding; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013574:Synaptosomes / Q000378:metabolism; D013997:Time Factors; D014162:Transfection; D014168:Transferrin / Q000378:metabolism; D033921:Vesicular Transport Proteins
D002352:Carrier Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D011993:Recombinant Fusion Proteins; C405513:SNPH protein, human; C000592961:Snph protein, rat; D014168:Transferrin; D033921:Vesicular Transport Proteins; C075637:amphiphysin; D005982:Glutathione Transferase; D034284:Dynamin I