Hydrolysis of DNA by Ce(IV)/EDTA complexes--the mechanism of DNA cleavage and design of artificial restriction enzymes.
Homogeneous Ce(IV) complex of EDTA promptly hydrolyzes oligonucleotides under physiological conditions. Moreover, the activity of Ce(IV)/EDTA for DNA hydrolysis is promoted by the addition of amines. When [Ce(IV)/EDTA] = 5 mumol dm3 and [ethylenediamine] = 100 mmol dm3, the catalytic activity is about 50 times as large as that of Ce(IV)/EDTA. The combination of Ce(IV)/EDTA and amines is eminent tools for the future molecular biology and biotechnology.
Yamamoto
Y
Y
Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 513-8904, Japan.
Igawa
T
T
Sumaoka
J
J
Komiyama
M
M
D002384:Catalysis; D002563:Cerium; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000737:chemistry* / Q000302:isolation & purification; D004262:DNA Restriction Enzymes / Q000737:chemistry; D015195:Drug Design; D004492:Edetic Acid; D005029:Ethylenediamines; D006868:Hydrolysis; D007202:Indicators and Reagents
D005029:Ethylenediamines; D007202:Indicators and Reagents; D002563:Cerium; C031234:ethylenediamine; D004247:DNA; D004492:Edetic Acid; D004262:DNA Restriction Enzymes