[Synthesis characterization and DNA binding spectroscopy studies of cobalt(III) mixed-polypyridyl complex].
Two complexes of[Co(phen)2PIP](ClO4)3.H2O and [Co(phen)2HPIP](ClO4)3.2H2O have been synthesized(PIP = 2-imidazo[4,5-f][1,10]phenanthroline, HPIP = 2-(2-hydroxyphenyl)-imidazo [4,5-f][1,10]phenanthroline) and characterized by elemental analysis, UV, IR and 1H NMR spectroscopy. The interaction of the complexes with calf thymus DNA have been studied using absorption and emission spectroscopy, DNA melting techniques and cyclic voltammetric. The compounds show absorption hypochromicity, fluorescence enhancement, DNA melting increasement when binding to CT.DNA. CV measurement shows that the shift in reduction potential and the change in peak current with addition of DNA. These results prove the compounds insert DNA base pairs. The shift of peak potential indicates the ion interaction mode between the drug and DNA. The binding constants of the compounds to DNA are 1.8 x 10(4) and 4.37 x 10(4) respectively.
Chen
Hui-li
HL
Institute of Molecular Science, Shanxi University, Taiyuan 030006, China.
Yang
Pin
P
D003035:Cobalt / Q000737:chemistry*; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004563:Electrochemistry; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D010618:Phenanthrolines / Q000138:chemical synthesis / Q000737:chemistry*; D010782:Photolysis; D013057:Spectrum Analysis
D009942:Organometallic Compounds; D010618:Phenanthrolines; D003035:Cobalt; D004247:DNA