[Expression of neurotrophin-4(NT-4) gene in baculovirus expression vector system].
OBJECTIVE
We selected baculovirus expression vector system to express human NT-4 with biological activity.


METHODS
The hNT-4 mature peptide-coding sequence is amplified by polymerase chain reaction (PCR), ligated to baculovirus expression vector PacGP67B, and expressed in the insert sf9 cell line.


RESULTS
The protein molecular weight of the expressed hNT-4 was about 15,000 by SDS-PAGE, and the hNT-4 antibody could react with this protein in the infected supernatant and total cell by western-blot. The activity of hNT-4 determined by PC12 cell line was definite.


CONCLUSIONS
The results may aid for studying the effect of the hNT-4 on basic medical research and clinical application.
Ma
X L
XL
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China.
Huang
B R
BR
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D005822:Genetic Vectors; D006801:Humans; D009414:Nerve Growth Factors / Q000096:biosynthesis* / Q000235:genetics; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics
D009414:Nerve Growth Factors; D011994:Recombinant Proteins; C073922:neurotrophin 4