A functional gene discovery in cell differentiation by hybrid ribozyme and siRNA libraries.
Recently, we developed a gene discovery system that can identify functional genes using a randomized hybrid ribozyme library. In this system, inhibition of the expression of a particular gene by active ribozymes was reflected by a change in a particular phenotype, the method allowed the identification of functional genes. In the case of identification of functional genes for apoptosis pathways, we identified many pro-apoptotic genes in TNF-alpha and Fas-mediated apoptosis pathways. In this study, we tried to identify the functional genes that are necessary for the retinoic acid (RA)-induced cell differentiation using randomized ribozyme and siRNA libraries. We succeeded to identify the several differentiation factors. Therefore, our gene discovery system based on randomized ribozyme and siRNA libraries are high potential to identify the differentiation and undifferentiation factors in the post genome era.
Kawasaki
Hiroaki
H
Department of Chemistry and Biotechnology, School of Engineering, University of Tokyo, 7-3-1 Hongo, Tokyo 113-8656, Japan.
Tsunemi
Masaru
M
Iyo
Mayu
M
Oshima
Keisuke
K
Minoshima
Hiroshi
H
Hamada
Akira
A
Onuki
Reiko
R
Suyama
Eigo
E
Taira
Kazunari
K
D002454:Cell Differentiation / Q000235:genetics*; D016337:RNA, Catalytic / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics*
D016337:RNA, Catalytic; D034741:RNA, Small Interfering