Genomic analysis of Mycobacterium tuberculosis complex strains used for production of purified protein derivative.
The genomes of the tuberculin production strains Mycobacterium bovis AN5 and Mycobacterium tuberculosis DT were compared to genome-sequenced tubercle bacilli by using DNA microarrays. Neither the AN5 nor DT strain suffered extensive gene deletions during in vitro passage. This suggests that bovine tuberculin made from M. bovis AN5 is suitable to detect infection with presently prevalent M. bovis strains.
Inwald
Jacqueline
J
TB Research Group, Veterinary Laboratories Agency (Weybridge), New Haw, Addlestone, Surrey KT15 3NB, United Kingdom.
Hinds
Jason
J
Palmer
Si
S
Dale
James
J
Butcher
Philip D
PD
Hewinson
R Glyn
RG
Gordon
Stephen V
SV
D017353:Gene Deletion; D016680:Genome, Bacterial; D018598:Minisatellite Repeats; D009163:Mycobacterium bovis / Q000235:genetics / Q000378:metabolism; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D013045:Species Specificity; D014373:Tuberculin / Q000235:genetics*
D014373:Tuberculin