Effects of beta-cyclodextrin-dextran polymer on stability properties of trypsin.
Dextran modified with the mono-6-pentylene-diamino-6-deoxy-beta-cyclodextrin derivative was evaluated as a thermoprotectant additive for trypsin. The optimum temperature for trypsin activity was increased by 7 degrees C in the presence of this polymer. The enzyme thermostability was increased from 48.5 to 64 degrees C over 10 min of incubation, and the activation free energy of thermoinactivation at 50 degrees C was increased by 4.1 kJ/mol in the presence of the additive. Trypsin was 6-fold more resistant to autolytic inactivation at alkaline pH in the presence of the polymer.
Fernández
Michael
M
Enzyme Technology Group, Center for Biotechnological Studies, University of Matanzas, Matanzas, C.P. 44740, Cuba.
Villalonga
Maria L
ML
Caballero
Julio
J
Fragoso
Alex
A
Cao
Roberto
R
Villalonga
Reynaldo
R
D000818:Animals; D002417:Cattle; D002482:Cellulose / Q000737:chemistry*; D003505:Cyclodextrins / Q000737:chemistry*; D003911:Dextrans / Q000737:chemistry*; D004789:Enzyme Activation; D004795:Enzyme Stability; D007700:Kinetics; D010179:Pancreas / Q000201:enzymology; D013696:Temperature; D014357:Trypsin / Q000138:chemical synthesis / Q000737:chemistry*
D003505:Cyclodextrins; D003911:Dextrans; C041953:cyclodextrin polymer; D002482:Cellulose; D014357:Trypsin