Identification and characterization of U1 small nuclear RNA genes from two crustacean isopod species.
Four different units containing three variants of the U1 snRNA gene have been identified in the genome of Asellus aquaticus and only one unit has been identified in the genome of Proasellus coxalis. All four identified U1 snRNA genes can be folded according to the proper secondary structure and possess the functionally useful conserved sequences. Moreover, in the 3 flanking regions, all genes present both the 3 box, a conserved sequence required for 3 processing of mature snRNA, and a polyadenylation signal which is unusual for these genes. The PCR products were used as probes in fluorescent in-situ hybridization (FISH) experiments to locate them on chromosomes of A. aquaticus and P. coxalis.
Barzotti
Rita
R
Dipartimento di Genetica e Biologia Molecolare, Università La Sapienza, 00185 Roma, Italia.
Pelliccia
Franca
F
Rocchi
Angela
A
D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence / Q000235:genetics*; D017931:DNA Primers; D017404:In Situ Hybridization, Fluorescence; D033321:Isopoda / Q000235:genetics*; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D012342:RNA, Small Nuclear / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D012342:RNA, Small Nuclear; C405483:U1 small nuclear RNA