title: Trans-kingdom conjugation between bacterial chromosome-less mini-cells and yeasts.
abstract: We introduced a novel convenient method to prepare chromosome-less mini-cells from cell mixture of Escherichia coli P678-54 by using penicillin. The mini-cells prepared by this method could successfully transferred their harboring conjugative plasmids into eukaryotic yeast cells by trans-kingdom conjugation.
authors.affiliation: Department of Biological Science, Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8526, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Fukumaru
authors.forename: K
authors.initials: K
authors.lastname: Yoshida
mesh_terms: D002876:Chromosomes, Bacterial / Q000235:genetics; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000235:genetics*; D005821:Genetic Techniques; D008856:Microscopy, Fluorescence; D010406:Penicillins; D010957:Plasmids / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D010406:Penicillins