New thermodynamic characterization and transition mechanism of DNA duplex formation.
A new transition mechanism of DNA duplex association was proposed and a segregated transition model (STM) was further derived. The experimental results in various molar ratios showed that the duplex association transition is imperfect and the thermodynamic properties and self-transition behavior of single strands exert a significant influence on DNA duplex formation.
Wu
P
P
High Technology Research Center, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan.
Sugimoto
N
N
D001483:Base Sequence; D002152:Calorimetry, Differential Scanning; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry; D006801:Humans; D009690:Nucleic Acid Conformation; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics
D004277:DNA, Single-Stranded; D004247:DNA