[Effect of human fetal liver cells on the rate of DNA and nRNA synthesis in regenerating rat liver].
The model of partial hepatectomy was utilized to investigate the influence of human fetal liver cells (FLCs) and of human embryo tissue (PCF) postnuclear cytoplasm fraction injection on the rate of DNA and nRNA synthesis in regenerating rat liver cells. Single infusion of FLCs and PCF into the spleen pulp of rats has been shown to increase the DNA synthesis 24 hours after the operation in 2.5 +/- 0.4 and 3.2 +/- 0.5 times respectively. A group of rats has been identified with no influence of the infusion on the DNA synthesis 24 hours after partial hepatectomy, this process having been even retarding in 48 hours after the operation. Meanwhile the FLCs and PCF infusion enhanced the intensity of nRNA synthesis in 72 hours after the operation in all the animal groups. The effect demonstrated is probably caused by the biologically active substances contained in the fetal tissues.
Ochenashko
O V
OV
Institute for Problems of Cryobiology and Cryomedicine of NAS of Ukraine, Kharkiv.
Bozhkov
A I
AI
Petrenko
A Iu
AIu
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis*; D016332:Fetal Tissue Transplantation; D006498:Hepatectomy; D022781:Hepatocytes / Q000166:cytology* / Q000502:physiology; D006801:Humans; D007700:Kinetics; D008099:Liver / Q000196:embryology; D008115:Liver Regeneration / Q000502:physiology*; D016031:Liver Transplantation / Q000379:methods; D015138:RNA, Nuclear / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000166:cytology / Q000378:metabolism
D015138:RNA, Nuclear; D004247:DNA