Trans-kingdom conjugation in vitro by using toluenized cells.
To establish in vitro trans-kingdom conjugation, we developed a novel direct cell PCR by using toluenized cells which permeate enzymes, primers and substrates. The cells was shown to be successful recipients in conjugation.
Tonomoto
Riko
R
Department of Biological Science, Graduate School of Science, Hiroshima University, Kagamiyama 1-3-1, Higashi-Hiroshima 739-8526, Japan.
Sonoda
Hiroyuki
H
Suzuki
Katsunori
K
Yoshida
Kazuo
K
D001419:Bacteria / Q000235:genetics*; D003227:Conjugation, Genetic; D005658:Fungi / Q000235:genetics*; D010957:Plasmids; D016133:Polymerase Chain Reaction; D014050:Toluene / Q000008:administration & dosage*
D014050:Toluene