Screening in vivo for RNA-binding peptides from combinatorial libraries.
We have modified a previously developed genetic assay system for RNA-polypeptide interactions in a attempt to more readily identify RNA-binding peptides. The first modification involved the design of a "complex" library that would contain a variety of RNA-binding polypeptides. The second modification involved the use of neomycin phosphotransferase (NPT II) as the reporter gene, therefore allowing "selection" of RNA-binding peptides by kanamycin resistance. The improved screening system should allow the identification of peptides that bind to a variety of RNA structures.
Harada
K
K
Department of Life Science, Tokyo Gakugei University, Koganei-shi, Tokyo 184-8501, Japan.
Horiya
S
S
Zehavi
H
H
Frankel
A D
AD
D020650:Combinatorial Chemistry Techniques; D015195:Drug Design; D017930:Genes, Reporter; D019868:Kanamycin Kinase / Q000235:genetics; D007613:Kanamycin Resistance / Q000235:genetics; D019151:Peptide Library; D012313:RNA / Q000737:chemistry / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
D019151:Peptide Library; D016601:RNA-Binding Proteins; D012313:RNA; D019868:Kanamycin Kinase