title: [Establishment of drug-resistance cell line of human glioma mediated by MGMT].
abstract: OBJECTIVE
  To establish a drug-resistance cell line of human glioma mediated by MGMT.
  
  
  METHODS
  Simulated the clinical usage of BCNU to establish a BCNU-resistant human glioma subline by cyclic exposing the U251 parent cells to a constant concentration of BCNU. The resistance index and the expression of MGMT mRNA of U251/BCNU were detected and compared the difference of in vitro proliferation between U251 and U251/BCNU.
  
  
  RESULTS
  A subline--U251/BCNU was successfully established in about 4-month culture, which had a stable resistance to BCNU. U251/BCNU cells showed 17-fold higher resistance to BCNU than did U251 cells by MTT assay, while U251/BCNU cells expressed MGMT mRNA. The doubling time of U251 and U251/BCNU had no statistical difference.
  
  
  CONCLUSIONS
  A drug-resistance cell line of human glioma mediated by MGMT is established, which could provide experimental basis for further studies on the resistance mechanism and reversal methods of glioma chemotherapy.
authors.affiliation: Department of Neurosurgery, Second Hospital of Shandong University, Jinan 250033, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Jin
authors.forename: Qing-lin
authors.initials: QL
authors.lastname: Zhang
authors.forename: Fu-sheng
authors.initials: FS
authors.lastname: Liu
authors.forename: Bao-an
authors.initials: BA
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Wei
authors.forename: Cheng-wei
authors.initials: CW
authors.lastname: Wang
authors.forename: Jian-xin
authors.initials: JX
authors.lastname: Kong
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000096:biosynthesis; D001932:Brain Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D015254:DNA Modification Methylases / Q000096:biosynthesis / Q000235:genetics; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005910:Glioma / Q000473:pathology*; D006801:Humans; D019853:O(6)-Methylguanine-DNA Methyltransferase / Q000378:metabolism / Q000502:physiology*
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D015254:DNA Modification Methylases; D019853:O(6)-Methylguanine-DNA Methyltransferase