Regulation of Notch signaling by a novel mechanism involving suppressor of hairless stability and carboxyl terminus-truncated notch.
Different amounts of Suppressor of Hairless (SuH)-dependent Notch (N) signaling is often used during animal development to produce two different tissues from a population of equipotent cells. During Drosophila melanogaster embryogenesis, cells with high amounts of this signaling differentiate the larval epidermis whereas cells with low amounts, or none, differentiate the central nervous system (CNS). The mechanism by which SuH-dependent N signaling is increased or decreased in these different cells is obscure. The developing epidermis is known to get enriched for the full-length N (NFull) and the developing CNS for the carboxyl terminus-truncated N (NdeltaCterm). Results described here indicate that this differential accumulation of N receptors is part of a mechanism that would promote SuH-dependent N signaling in the developing epidermis but suppress it in the developing CNS. This mechanism involves SuH-dependent stability of NFull, NFull-dependent accumulation of SuH, stage specific stability of SuH, and NdeltaCterm-dependent loss of SuH and NFull.
Wesley
Cedric S
CS
Department of Microbiology and Molecular Genetics, The University of Vermont, Burlington, Vermont 05405, USA.
Mok
Lee-Peng
LP
D000818:Animals; D015153:Blotting, Western; D002490:Central Nervous System / Q000196:embryology; D029721:Drosophila Proteins; D004331:Drosophila melanogaster; D010450:Endopeptidases / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D011233:Precipitin Tests; D011480:Protease Inhibitors / Q000494:pharmacology; D017434:Protein Structure, Tertiary; D012313:RNA / Q000378:metabolism; D051880:Receptors, Notch; D015398:Signal Transduction; D013696:Temperature; D013997:Time Factors
D029721:Drosophila Proteins; D008565:Membrane Proteins; C497759:N protein, Drosophila; D011480:Protease Inhibitors; D051880:Receptors, Notch; D012313:RNA; D010450:Endopeptidases