title: Visualizing phosphoinositide signalling in single neurons gets a green light.
abstract: There is now substantial evidence, from single-cell imaging, that complex patterns of release from Ca(2+) stores play an important role in regulating synaptic efficacy and plasticity. Moreover, the major mechanism of store release depends on the generation of inositol 1,4,5-trisphosphate [Ins(1,4,5)P(3)] through the action of phospholipase(s) C on phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P(2)], and several neurotransmitters can enhance receptor-mediated activation of this enzyme. The recent development of techniques to image real-time changes in PtdIns(4,5)P(2) hydrolysis according to generation of Ins(1,4,5)P(3) and diacylglycerol in single cells has significantly advanced our ability to investigate these signalling pathways, particularly in relation to single-cell Ca(2+) signals. This article reviews these new approaches and how they have provided novel insights into mechanisms underlying spatio-temporal Ca(2+) signals and phospholipase C activation in neurons.
authors.affiliation: Department of Cell Physiology and Pharmacology, University of Leicester, Maurice Shock Medical Sciences Building, University Road, LE1 9HN, Leicester, UK. srn@le.ac.uk
authors.forename: Stefan R
authors.initials: SR
authors.lastname: Nahorski
authors.forename: Kenneth W
authors.initials: KW
authors.lastname: Young
authors.forename: R A
authors.initials: RA
authors.lastname: John Challiss
authors.forename: Mark S
authors.initials: MS
authors.lastname: Nash
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D020013:Calcium Signaling / Q000502:physiology*; D004594:Electrophysiology; D005455:Fluorescent Antibody Technique / Q000379:methods; D015544:Inositol 1,4,5-Trisphosphate / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D019269:Phosphatidylinositol 4,5-Diphosphate; D015544:Inositol 1,4,5-Trisphosphate