2-Methyl-5-tert-butylthiophenol--an odorless deprotecting reagent useful in synthesis of oligonucleotides and their analogs.
A solution of 2-methyl-5-tert-butylthiophenol and triethylamine in acetonitrile efficiently removed the methyl protecting groups from phosphate and phosphorothioate triesters and yielded oligonucleotides of high quality. This inexpensive odorless liquid is not toxic and is a suitable replacement for hazardous thiophenol and other reagents often used for this purpose.
Kumar
R Krishna
RK
Isis Pharmaceuticals, Carlsbad, California 92008, USA.
Cole
Douglas L
DL
Ravikumar
Vasulinga T
VT
D000097:Acetonitriles / Q000737:chemistry; D005021:Ethylamines / Q000737:chemistry; D009812:Odorants; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D010636:Phenols / Q000737:chemistry*; D013329:Structure-Activity Relationship; D013438:Sulfhydryl Compounds / Q000737:chemistry*
C482609:2-methyl-5-tert-butylthiophenol; D000097:Acetonitriles; D005021:Ethylamines; D009841:Oligonucleotides; D010636:Phenols; D013438:Sulfhydryl Compounds; C042983:thiophenol; C016162:triethylamine; C032159:acetonitrile