Human cytomegalovirus induces apoptosis in promonocyte THP-1 cells but not in promyeloid HL-60 cells.
The effect of human cytomegalovirus (HCMV) infection on the viability of the cells in the monocyte/myeloid lineage was investigated. Two cell lines at different stages in the differentiation pathway, the less differentiated promyeloid HL-60 and the more differentiated promonocyte THP-1 cells, were used in this study. While the viability of THP-1 cells was significantly impaired by HCMV infection, the viability of HL-60 cells was not affected. The decrease in the viability of THP-1 cells appears to result from the increase in apoptosis following HCMV infection. Interestingly, HL-60 cells were more sensitive than THP-1 cells to the apoptotic effect of other apoptogenic agents such as ultraviolet irradiation and hydrogen peroxide. When HL-60 cells were induced to differentiate by treating cells with 12-O-tetradecanoyl-phorbol 13-acetate (TPA), HCMV infection induced an increase in apoptosis of the differentiated HL-60 cells by TPA. Therefore, HCMV-induced apoptosis in the cells of the myeloid/monocyte lineage appears to depend on the degree of cell differentiation.
Moon
Myung S
MS
The Division of Life Sciences, College of Natural Sciences, Chungbuk National University, Cheongju, Chungbuk, 361-763, South Korea.
Lee
Gyu Cheol
GC
Kim
Jin H
JH
Yi
Hyun A
HA
Bae
Yong S
YS
Lee
Chan H
CH
D017209:Apoptosis; D002452:Cell Count; D002454:Cell Differentiation; D002460:Cell Line; D002470:Cell Survival; D003587:Cytomegalovirus / Q000235:genetics / Q000502:physiology*; D053938:DNA Fragmentation; D015967:Gene Expression Regulation, Viral; D018922:HL-60 Cells; D006801:Humans; D008564:Membrane Potentials; D008928:Mitochondria / Q000502:physiology; D009000:Monocytes / Q000166:cytology* / Q000821:virology*; D022423:Myeloid Cells / Q000166:cytology* / Q000821:virology*