title: Cutting edge: SR-PSOX/CXC chemokine ligand 16 mediates bacterial phagocytosis by APCs through its chemokine domain.
abstract: SR-PSOX and CXC chemokine ligand (CXCL)16, which were originally identified as a scavenger receptor and a transmembrane-type chemokine, respectively, are indicated to be identical. In this study, we demonstrate that membrane-bound SR-PSOX/CXCL16 mediates adhesion and phagocytosis of both Gram-negative and Gram-positive bacteria. Importantly, our prepared anti-SR-PSOX mAb, which suppressed chemotactic activity of SR-PSOX, significantly inhibited bacterial phagocytosis by human APCs including dendritic cells. Various scavenger receptor ligands inhibited the bacterial phagocytosis of SR-PSOX. In addition, the recognition specificity for bacteria was determined by only the chemokine domain of SR-PSOX/CXCL16. Thus, SR-PSOX/CXCL16 may play an important role in facilitating uptake of various pathogens and chemotaxis of T and NKT cells by APCs through its chemokine domain.
authors.affiliation: Graduate School of Biostudies and Institute for Virus Research, Kyoto University, Sakyo-ku, Kyoto, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Shimaoka
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakayama
authors.forename: Noriaki
authors.initials: N
authors.lastname: Kume
authors.forename: Shu
authors.initials: S
authors.lastname: Takahashi
authors.forename: Junko
authors.initials: J
authors.lastname: Yamaguchi
authors.forename: Manabu
authors.initials: M
authors.lastname: Minami
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Hayashida
authors.forename: Toru
authors.initials: T
authors.lastname: Kita
authors.forename: Jun
authors.initials: J
authors.lastname: Ohsumi
authors.forename: Osamu
authors.initials: O
authors.lastname: Yoshie
authors.forename: Shin
authors.initials: S
authors.lastname: Yonehara
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology*; D001422:Bacterial Adhesion / Q000187:drug effects / Q000276:immunology; D019556:COS Cells; D000075743:Chemokine CXCL16; D054427:Chemokine CXCL6; D019743:Chemokines, CXC / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D016264:Dextran Sulfate / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D005260:Female; D006801:Humans; D008024:Ligands; D008077:Lipoproteins, LDL / Q000494:pharmacology; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010084:Oxidation-Reduction; D010587:Phagocytosis / Q000276:immunology*; D017434:Protein Structure, Tertiary / Q000502:physiology; D011971:Receptors, Immunologic / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; D051122:Scavenger Receptors, Class B; D013211:Staphylococcus aureus / Q000187:drug effects / Q000276:immunology / Q000378:metabolism
chemical_list: C423727:CXCL16 protein, human; D000075743:Chemokine CXCL16; D054427:Chemokine CXCL6; D019743:Chemokines, CXC; C498926:Cxcl16 protein, mouse; D008024:Ligands; D008077:Lipoproteins, LDL; D008565:Membrane Proteins; D011971:Receptors, Immunologic; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; C454739:Scarb1 protein, mouse; D051122:Scavenger Receptors, Class B; C099252:oxidized low density lipoprotein; D016264:Dextran Sulfate