title: [Expression of rat protamine gene in MEL cells].
abstract: OBJECTIVE
  To investigate the expression of rat protamine (RP) gene in MEL cells and the effect on cell growth.
  
  
  METHODS
  Eukaryotic expression plasmid pCR-3.1-RP was constructed and transfected into MEL cells. The changes of cell growth rate, mitotic index and colony-forming rate in semi-solid medium were investigated.
  
  
  RESULTS
  Transfected MEL cells showed lower growth rate, mitotic index and colony-forming rate. Volumes of cells were reduced and reduction of RNA transcription was observed.
  
  
  CONCLUSIONS
  These results suggest that expression of RP in MEL cell may inhibit the cell growth and proliferation.
authors.affiliation: Institute of Materia Medica, CAMS, PUMC, Beijing 100050, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.forename: Ren-ren
authors.initials: RR
authors.lastname: Fei
authors.forename: Xiao-fang
authors.initials: XF
authors.lastname: Wu
authors.forename: Ye-hua
authors.initials: YH
authors.lastname: Ge
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Ma
authors.forename: Dai-shu
authors.initials: DS
authors.lastname: Han
authors.forename: She-pu
authors.initials: SP
authors.lastname: Xue
mesh_terms: D000818:Animals; D002455:Cell Division; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics* / Q000473:pathology; D010957:Plasmids; D011479:Protamines / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D011479:Protamines