Identification of the lantibiotic nisin Q, a new natural nisin variant produced by Lactococcus lactis 61-14 isolated from a river in Japan.
Lactococcus lactis 61-14 isolated from river water produced a bacteriocin active against a wide range of Gram-positive bacteria. N-terminal amino acid sequencing, mass spectral analysis of the purified bacteriocin, and genetic analysis using nisin-specific primers showed that the bacteriocin was a new natural nisin variant, termed nisin Q. Nisin Q and nisin A differ in four amino acids in the mature peptide and two in the leader sequence.
Zendo
Takeshi
T
Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan.
Fukao
Masanori
M
Ueda
Kyoko
K
Higuchi
Tomoko
T
Nakayama
Jiro
J
Sonomoto
Kenji
K
D000595:Amino Acid Sequence; D001430:Bacteriocins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D001483:Base Sequence; D014644:Genetic Variation / Q000235:genetics*; D007564:Japan; D013294:Lactococcus lactis / Q000737:chemistry*; D008969:Molecular Sequence Data; D009561:Nisin / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D011487:Protein Conformation; D045483:Rivers / Q000382:microbiology*
D001430:Bacteriocins; D009561:Nisin