Liquid chromatographic separation of the enantiomers of beta-amino acids on a ligand exchange chiral stationary phase.
A liquid chromatographic ligand exchange chiral stationary phase (CSP) derived from (S)-leucinol was applied in the separation of the enantiomers of 12 beta-amino acids. The resolution was quite successful especially for the enantiomers of beta-amino acids containing aromatic functional group in the side chain. The chromatographic resolution behaviors were dependent on the organic modifier and Cu(II) concentration in aqueous mobile phase and the column temperature.
Hyun
Myung Ho
MH
Department of Chemistry, Pusan National University, Pusan 609-735, Republic of Korea. mhhyun@pusan.ac.kr
Han
Sang Cheol
SC
Whangbo
Sung Hee
SH
D000596:Amino Acids / Q000737:chemistry / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000379:methods; D003300:Copper / Q000737:chemistry; D008024:Ligands; D000432:Methanol / Q000737:chemistry; D015394:Molecular Structure; D012997:Solvents; D013237:Stereoisomerism; D013696:Temperature; D014867:Water / Q000737:chemistry
D000596:Amino Acids; D008024:Ligands; D012997:Solvents; D014867:Water; D003300:Copper; D000432:Methanol