title: Establishment of a monoclonal antibody for human LXRalpha: Detection of LXRalpha protein expression in human macrophages.
abstract: Liver X activated receptor alpha (LXRalpha) forms a functional dimeric nuclear receptor with RXR that regulates the metabolism of several important lipids, including cholesterol and bile acids. As compared with RXR, the LXRalpha protein level in the cell is low and the LXRalpha protein itself is very hard to detect. We have previously reported that the mRNA for LXRalpha is highly expressed in human cultured macrophages. In order to confirm the presence of the LXRalpha protein in the human macrophage, we have established a monoclonal antibody against LXRalpha, K-8607. The binding of mAb K-8607 to the human LXRalpha protein was confirmed by a wide variety of different techniques, including immunoblotting, immunohistochemistry, and electrophoretic mobility shift assay (EMSA). By immunoblotting with this antibody, the presence of native LXR protein in primary cultured human macrophage was demonstrated, as was its absence in human monocytes. This monoclonal anti-LXRalpha antibody should prove to be a useful tool in the analysis of the human LXRalpha protein.
authors.affiliation: Department of Life Sciences, The University of Tokyo Research Center for Advanced Science and Technology (RCAST), Tokyo, Japan. kodama@lsbm.org
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Watanabe
authors.forename: Toshiya
authors.initials: T
authors.lastname: Tanaka
authors.forename: Yasutoshi
authors.initials: Y
authors.lastname: Uchiyama
authors.forename: Tetsu
authors.initials: T
authors.lastname: Takeno
authors.forename: Akashi
authors.initials: A
authors.lastname: Izumi
authors.forename: Hisahiko
authors.initials: H
authors.lastname: Yamashita
authors.forename: Junko
authors.initials: J
authors.lastname: Kumakura
authors.forename: Hiroko
authors.initials: H
authors.lastname: Iwanari
authors.forename: Jiang
authors.initials: J
authors.lastname: Shu-Ying
authors.forename: Makoto
authors.initials: M
authors.lastname: Naito
authors.forename: David J
authors.initials: DJ
authors.lastname: Mangelsdorf
authors.forename: Takao
authors.initials: T
authors.lastname: Hamakubo
authors.forename: Tatsuhiko
authors.initials: T
authors.lastname: Kodama