Functional analysis of the pro-apoptotic factor Bax using hammerhead ribozymes.
A pro-apoptotic protein Bax is a Bcl-2 family member and forms homodimers and also heterodimerizes with death antagonists, Bcl-2 and Bcl-XL. To elucidate the detail of function of Bax in cells, we constructed a hammerhead ribozyme targeted to the Bax mRNA. The level of Bax protein in Hela-K cells expressing Bax-ribozyme was decreased compared with that of wild type Hela-K cells. Therefore, the Bax-ribozyme should be useful for the future investigations of the details of apoptosis pathway.
Takeda
H
H
National Institute for Advanced Interdisciplinary Research (NAIR), Institute of Applied Biochemistry, University of Tsukuba, Tsukuba Science City, Japan.
Kawasaki
H
H
Taira
K
K
D017209:Apoptosis / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000502:physiology*; D019253:Proto-Oncogene Proteins c-bcl-2; D016337:RNA, Catalytic / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D051028:bcl-2-Associated X Protein
C494826:BAX protein, human; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D016337:RNA, Catalytic; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein; C427204:hammerhead ribozyme