title: Transcriptional regulation of the c-jun gene by AP-1 repressor protein JDP2 during the differentiation of F9 cells.
abstract: ATF-2 and p300 cooperate in the activation of transcription of the c-jun gene during the differentiation of F9 cells. We show here that a repressor of AP-1, JDP2 (Jun dimerization protein 2), inhibits the transactivation of the c-jun gene by ATF-2 and p300, by recruitment of a histone deacetylase complex, thereby repressing the retinoic acid (RA)-induced transcription of the c-jun gene and inhibiting the RA-mediated differentiation of F9 cells. Furthermore, JDP2/HDAC complex was replaced by p300 complex on the DRE (differentiation response element) of the c-jun promoter within 24 h after the start of exposure of cells to RA, with an accompanying change in the histone-acetylation status of the chromatin, indicating that the initiation of transcription of the c-jun gene was controled by sequential action of HDAC(s) and HAT(s) on c-jun promoter.
authors.affiliation: Gene Engineering Division, RIKEN (Institute of Physical & Chemical Research), Bioresource Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan.
authors.forename: Chunyuan
authors.initials: C
authors.lastname: Jin
authors.forename: Hongjie
authors.initials: H
authors.lastname: Li
authors.forename: Hideyo
authors.initials: H
authors.lastname: Ugai
authors.forename: Takehide
authors.initials: T
authors.lastname: Murata
authors.forename: Kazunari K
authors.initials: KK
authors.lastname: Yokoyama
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D024202:Electrophoretic Mobility Shift Assay; D005786:Gene Expression Regulation / Q000502:physiology*; D016758:Genes, jun; D051379:Mice; D012097:Repressor Proteins / Q000502:physiology*; D018808:Transcription Factor AP-1 / Q000037:antagonists & inhibitors*; D014158:Transcription, Genetic / Q000502:physiology*; D014407:Tumor Cells, Cultured
chemical_list: C491386:Jundp2 protein, mouse; D012097:Repressor Proteins; D018808:Transcription Factor AP-1