[Association of HLA-DRB genes with Vogt-Koyanagi-Harada syndrome in a Chinese Han population].
OBJECTIVE
To investigate the molecular genetic background of VKH syndrome in Han Chinese by HLA-DRB genotyping.


METHODS
HLA-DRB genotyping was performed by PCR-SSOP method, then DR4 alleles were analyzed by SSOP and RFLP methods.


RESULTS
The frequency of DRB1 * 0405 was significantly higher in VKH patients than that in normal controls (80.4% vs 8.1%, Pc < 10(-7), RR = 46.7), so was the frequency of DRB1 * 15 in DRB1 * 0405 negative individuals (Pc < 0.05, RR = 6.0). On the other hand, the frequencies of DRB1 * 14 and DRB1 * 08 were significantly lower in VKH patients than that in normal controls. Comparing the clinical features between DRB1 * 0405 positive and negative VKH patients, no significant differences were found.


CONCLUSIONS
(1) DRB1 * 0405 and DRB1 * 15 are closely associated with the susceptibility to VKH syndrome, DRB1 * 0405 may be the major susceptible gene and DRB1 * 15 may be the minor; (2) DRB1 * 14 and DRB1 * 08 are negatively associated with the susceptibility to VKH syndrome, suggesting that they may be the resistant genes; (3) DRB1 * 0405 is not related to the clinical features, incidence of ocular complications as well as visual prognosis.
Zhang
M
M
Department of Ophthalmology, PUMC Hospital, CAMS, PUMC, Beijing 100730, China.
Qiu
C
C
Hu
T
T
D000328:Adult; D000368:Aged; D005260:Female; D006684:HLA-DR Antigens / Q000235:genetics*; D015804:HLA-DR4 Antigen / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D014607:Uveomeningoencephalitic Syndrome / Q000235:genetics* / Q000276:immunology
D006684:HLA-DR Antigens; D015804:HLA-DR4 Antigen