title: Transposases are responsible for the target specificity of IS1397 and ISKpn1 for two different types of palindromic units (PUs).
abstract: Insertion sequences (IS)1397 and ISKpn1, found in Escherichia coli and Klebsiella pneumoniae, respectively, are IS3 family members that insert specifically into short palindromic repeated sequences (palindromic units or PUs). In this paper, we first show that although PUs are naturally absent from extrachromosomal elements, both ISs are able to transpose from the chromosome or from a plasmid into PUs artificially introduced into target plasmids. We also show that ISKpn1 target specificity is restricted to K.pneumoniae Z1 PU type, whereas IS1397 target specificity is less stringent since the IS targets the three E.coli Y, Z1 and Z2 PU types indifferently. Experiments of transposition of both ISs driven by both transposases demonstrate that the inverted repeats flanking the ISs are not responsible for this target specificity, which is entirely due to the transposase itself. Implications on ISs evolution are presented.
authors.affiliation: Unité de Programmation Moléculaire et Toxicologie Génétique, CNRS URA 1444 Institut Pasteur, 25 rue du Dr Roux, F-75724 Paris Cedex 15, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Wilde
authors.forename: Frédéric
authors.initials: F
authors.lastname: Escartin
authors.forename: Susumu
authors.initials: S
authors.lastname: Kokeguchi
authors.forename: Patricia
authors.initials: P
authors.lastname: Latour-Lambert
authors.forename: Aude
authors.initials: A
authors.lastname: Lectard
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Clément
mesh_terms: D001483:Base Sequence; D002876:Chromosomes, Bacterial / Q000235:genetics; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D007711:Klebsiella pneumoniae / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011995:Recombination, Genetic; D016415:Sequence Alignment; D019895:Transposases / Q000378:metabolism*
chemical_list: D004251:DNA Transposable Elements; D019895:Transposases