Telomerase-inhibitory effects of sugar-modified nucleotide analogs.
Telomerase is an endogenous reverse transcriptase that uses its internal RNA moiety as a template for the synthesis of telomere repeats, thus maintaining telomere length. To study the susceptibility of telomerase to sugar-modified nucleotide analogs, inhibition by arabinofuranosylguanine 5'-triphosphate (araGTP), 3'-azido-2',3'-dideoxyguanosine 5'-triphosphate (AZdGTP), 2',3'-dideoxy-2'-fluoroarabino-furanosylguanine 5'-triphosphate (FaraGTP), and their thymine counterparts was investigated. Among these compounds, all dGTP analogs showed potent inhibitory activity against human telomerase. Conversely, dTTP analogs showed moderate or weak inhibition. Partially purified telomerase from cherry salmon testis utilized ddGTP and AZdGTP as substrates into the 3'-terminus of DNA.
Jinmei
Hiroshi
H
Department of Biological Sciences, Teikyo University of Science and Technology, Uenohara, Yamanashi 409-0193, Japan.
Takahashi
Hazuki
H
Amano
Rie
R
Suzuki
Kaori
K
Saneyoshi
Mineo
M
Yamaguchi
Toyofumi
T
D000818:Animals; D002241:Carbohydrates / Q000737:chemistry*; D008297:Male; D009711:Nucleotides / Q000737:chemistry*; D012474:Salmon; D019098:Telomerase / Q000037:antagonists & inhibitors*; D013737:Testis / Q000201:enzymology
D002241:Carbohydrates; D009711:Nucleotides; D019098:Telomerase