Effects of sympathectomy on experimentally induced pulpal inflammation and periapical lesions in rats.
The role of sympathetic nerves in bone physiology is largely unknown. Recent studies have shown a correlation between sympathectomy and bone remodeling. The present experiments were aimed to study the effects of unilateral sympathectomy on bilateral experimentally induced pulpitis and periapical lesions in the rat maxilla and mandible. Adult male Sprague-Dawley rats were used. Experimental rats (n=11) had the right superior cervical ganglion surgically removed (SCGx) and control rats (n=5) had sham surgery. Pulpal inflammation and periapical bone lesions in the maxilla and mandible were created 14 days later in both experimental and control rats by exposing the dental pulp in the first and second molars and leaving them open to the oral microflora. The rats were perfused 20 days thereafter and the jaws processed for immunohistochemistry with neuropeptide Y (NPY) and ED1 as primary antibodies. Sympathectomy resulted in an almost complete loss of NPY-immunoreactive (IR) fibers in the right SCGx jaws. In the non-sympathectomized (non-SCGx) left side and in the control rats, sprouting of NPY-IR fiber was observed in the inflamed pulp tissue adjacent to reparative dentin formation and in the apical periodontal ligament of the partially necrotic first molars. Significantly more ED1-IR osteoclasts were found in the resorptive lacunae lining the periphery of the periapical lesions on the SCGx side compared with the non-SCGx side (P<0.04) and the controls (P<0.03). The size of the periapical lesions were larger on the SCGx side compared with the non-SCGx side (P<0.03) in the mandible, but not in the maxilla. We conclude that inflammation causes sprouting of NPY-IR nerve fibers and that unilateral removal of the SCG increases both the area of the periapical lesions and the number of osteoclasts in the inflamed region.
Haug
S R
SR
Department of Physiology, University of Bergen, Arstadveien 19, 5009 Bergen, Norway. sivakami.rethnam@fys.uib.no
Heyeraas
K J
KJ
D000818:Animals; D000911:Antibodies, Monoclonal; D002452:Cell Count; D007150:Immunohistochemistry; D007249:Inflammation; D008297:Male; D008334:Mandible / Q000378:metabolism*; D008437:Maxilla / Q000378:metabolism*; D009478:Neuropeptide Y / Q000378:metabolism; D010010:Osteoclasts / Q000378:metabolism*; D011671:Pulpitis / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017783:Superior Cervical Ganglion / Q000473:pathology / Q000601:surgery; D013562:Sympathectomy; D013564:Sympathetic Nervous System / Q000378:metabolism* / Q000601:surgery
D000911:Antibodies, Monoclonal; D009478:Neuropeptide Y