title: Chitosan-alginate multilayer beads for gastric passage and controlled intestinal release of protein.
abstract: Chitosan-alginate beads loaded with a model protein, bovine serum albumin (BSA) were investigated to explore the temporary protection of protein against acidic and enzymatic degradation during gastric passage. Optimum conditions were established for preparation of homogenous, spherical, and smooth chitosan-alginate beads loaded with BSA. Multilayer beads were prepared by additional treatment with either chitosan or alginate or both. The presence of chitosan in the coagulation bath during bead preparation resulted in increased entrapment of BSA. During incubation in simulated gastric fluid (SGF pH 1.2), the beads showed swelling and started to float but did not show any sign of erosion. Inclusion of pepsin in the gastric fluid did not show a further effect on the properties of the beads. Release studies were done in simulated gastric fluid (SGF pH 1.2) and subsequently in simulated intestinal fluid (SIF pH 7.5) to mimic the physiological gastrointestinal conditions. After transfer to intestinal fluid, the beads were found to erode, burst, and release the protein. Microscopic and macroscopic observations confirmed that the release of protein was brought about by the burst of beads. Chitosan-reinforced calcium-alginate beads showed delay in the release of BSA. The multilayer beads disintegrated very slowly. The enzymes pepsin and pancreatin did not change the characteristics of BSA-loaded chitosan-alginate beads. Single layer chitosan-alginate beads released 80-90% of the model protein within 12h while multilayer beads released only 40-50% in the same period of time. The release from chitosan-alginate beads and multilayer beads in SIF was further delayed without prior incubation in SGF. It is concluded that alginate beads reinforced with chitosan offer an excellent perspective for controlled gastrointestinal passage of protein drugs.
authors.affiliation: Bioprocess Technology Program, Asian Institute of Technology, Klong Luang, Bangkok, Thailand.
authors.forename: Anil K
authors.initials: AK
authors.lastname: Anal
authors.forename: Deepak
authors.initials: D
authors.lastname: Bhopatkar
authors.forename: Seiichi
authors.initials: S
authors.lastname: Tokura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tamura
authors.forename: Willem F
authors.initials: WF
authors.lastname: Stevens
mesh_terms: D000464:Alginates / Q000737:chemistry*; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry*; D048271:Chitosan; D003692:Delayed-Action Preparations; D020723:Glucuronic Acid / Q000737:chemistry*; D006603:Hexuronic Acids / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D010194:Pancreatin / Q000737:chemistry; D010316:Particle Size; D010434:Pepsin A / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000008:administration & dosage* / Q000737:chemistry*; D012995:Solubility
chemical_list: D000464:Alginates; D003692:Delayed-Action Preparations; D006603:Hexuronic Acids; D002686:Chitin; D012710:Serum Albumin, Bovine; D010194:Pancreatin; D020723:Glucuronic Acid; D048271:Chitosan; D010434:Pepsin A