[Expression of cyclooxygenase-2 mRNA and identification of its spliceriant in human myometrium obtained from women in labor].
OBJECTIVE
To investigate the expression of cyclooxygenase-2 (COX-2) in human lower segments of myometrium obtained from women in labor and those not in labor and identify the splice variant of COX-2.


METHODS
Reverse transcriptase-polymerase chain reaction (RT-PCR) was used for investigating the expression of COX-2. According to the sequence of rat COX-2 splice variant, the primers were designed and synthesized, then the splice variant of COX-2 in human myometrium from woman in labor was identified, cloned into vector and sequenced.


RESULTS
The results showed that (1) The Expression of COX-2 mRNA was lower in human myometrium obtained from women who were not in labor than those in labor. (2) A new band of COX-2 was obtained in myometrium from a woman in labor. The fragment includes an unspliced intron, which locates between exons 7 and 8.


CONCLUSIONS
COX-2 gene is not only expressed highly in human myometrium from women in labor, but also produced splicing variant by alternative splicing.
Huang
Yin-ping
YP
Department of Obstetrics and Gynecology, First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China.
Ye
Du-yun
DY
Wu
Ping
P
Huang
Yun-feng
YF
Sun
Wei
W
Ying
Hong-gang
HG
Pan
Qian
Q
Wan
Jing-yuan
JY
Yuan
Ping
P
Zhang
Dai-juan
DJ
D000328:Adult; D017398:Alternative Splicing / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D051546:Cyclooxygenase 2; D018076:DNA, Complementary; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D007743:Labor, Obstetric / Q000378:metabolism*; D008565:Membrane Proteins; D009215:Myometrium / Q000201:enzymology*; D011247:Pregnancy; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018076:DNA, Complementary; D007527:Isoenzymes; D008565:Membrane Proteins; D012333:RNA, Messenger; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases