title: Pro-oxidative activity of nitroxides in their reactions with glutathione.
abstract: Nitroxides are unreactive towards glutathione in vitro. Interaction of nitroxides with peroxynitrite does not lead to a significant loss of their electron paramagnetic resonance (EPR) signal. However, addition of peroxynitrite to a solution containing glutathione and nitroxides induces an irreversible disappearance of EPR signal of nitroxides and augmentation of glutathione oxidation which is a pro-oxidant effect of these compounds. Nitroxide loss leading to the formation of amine derivatives is initiated by products of glutathione oxidation by peroxynitrite. The pro-oxidant action of nitroxides at micromolar concentrations may be important in view of the proposed use of these compounds as antioxidants.
authors.affiliation: Department of Molecular Biophysics, University of Łodź, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Głebska
authors.forename: Janusz
authors.initials: J
authors.lastname: Skolimowski
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Kudzin
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Gwoździński
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Grzelak
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Bartosz
mesh_terms: D004305:Dose-Response Relationship, Drug; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D005978:Glutathione / Q000737:chemistry / Q000378:metabolism*; D013058:Mass Spectrometry; D008956:Models, Chemical; D009569:Nitric Oxide; D016877:Oxidants / Q000737:chemistry / Q000494:pharmacology*; D030421:Peroxynitrous Acid / Q000138:chemical synthesis; D013997:Time Factors
chemical_list: D005609:Free Radicals; D016877:Oxidants; D030421:Peroxynitrous Acid; D009569:Nitric Oxide; D005978:Glutathione