[Differentially expressed genes in immunosuppressive and healthy mice after intranasal inoculation of Aspergillus fumigatus].
OBJECTIVE
To screen out the differential expression genes between the immune suppressive host and healthy host after intranasal inoculation of Aspergillus fumigatus (A fumigatus).


METHODS
Six immune suppressive mice and 6 healthy mice were inoculated with A, fumigatus intranasally. Three days after the inoculation the mice were killed and autopsied. The total RNA of the lungs was isolated. Gene chip technology was used to examine the differentially expressed genes.


RESULTS
66 differentially expressed genes were screened out of the 4098 genes. In the experimental group the genes encoding CD22, CD53, and 24p3 and the genes related to engulfment and digestion of macrophage or monocytes were up-regulated. The genes encoding surfactant associated protein C, immunoglobulin lambda chain and the genes related to pulmonary metabolism were down-regulated. The genes encoding IL18, CD8, and CD28 were not differentially expressed.


CONCLUSIONS
The up-regulation of CD22 gene may be one of the ways by which the host defense is inhibited. CD53 may take part in the regulation of engulfment of phagocytes. 24p3 protein, an acute stress protein, protects the host from excessive inflammatory response.
Wang
Wen-ling
WL
Department of Dermatology, Peking University First Hospital, Research Center for Medical Mycology, Peking University, Beijing 100034, China.
Li
Ruo-yu
RY
Zhu
Xue-jun
XJ
Wang
Duan-li
DL
D000209:Acute-Phase Proteins / Q000235:genetics; D000818:Animals; D015703:Antigens, CD / Q000235:genetics; D000944:Antigens, Differentiation, B-Lymphocyte / Q000235:genetics; D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics; D001228:Aspergillosis / Q000235:genetics* / Q000276:immunology; D001232:Aspergillus fumigatus; D015815:Cell Adhesion Molecules / Q000235:genetics; D016867:Immunocompromised Host / Q000235:genetics* / Q000276:immunology; D037102:Lectins / Q000235:genetics; D000071068:Lipocalin-2; D054834:Lipocalins; D008172:Lung Diseases, Fungal / Q000235:genetics* / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D020411:Oligonucleotide Array Sequence Analysis; D015513:Oncogene Proteins / Q000235:genetics; D051918:Sialic Acid Binding Ig-like Lectin 2; D060265:Tetraspanin 25; D015854:Up-Regulation
D000209:Acute-Phase Proteins; D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D000945:Antigens, Differentiation, T-Lymphocyte; C497868:Cd22 protein, mouse; C554895:Cd53 protein, mouse; D015815:Cell Adhesion Molecules; D037102:Lectins; D000071068:Lipocalin-2; D054834:Lipocalins; D015513:Oncogene Proteins; D051918:Sialic Acid Binding Ig-like Lectin 2; D060265:Tetraspanin 25; C071332:Lcn2 protein, mouse