Determination of urinary hippuric acid by micellar electrokinetic capillary chromatography.
We propose a method for the simultaneous determination of hippuric acid (HA) and creatinine based on capillary micellar electrokinetic chromatography. Experimental conditions were 20 mM sodium phosphate, pH 7.20, 25 mM sodium dodecyl sulfate, 5% (v/v) acetonitrile. Electropherograms evidenced HA and creatinine peaks in less than 12 min. The method showed good linearity for both analytes and satisfactory within-day precision. The present method, which is accurate, sensitive, rapid and simple, may be applied to single-spot urine samples.
Zuppi
Cecilia
C
Institute of Biochemistry and Clinical Biochemistry, Catholic University of Rome, Rome, Italy. czuppi@rm.unicatt.it
Rossetti
Diana Valeria
DV
Vitali
Alberto
A
Vincenzoni
Federica
F
Giardina
Bruno
B
Castagnola
Massimo
M
Messana
Irene
I
D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003404:Creatinine / Q000652:urine; D005919:Glomerular Filtration Rate; D006626:Hippurates / Q000652:urine*; D009682:Magnetic Resonance Spectroscopy; D015203:Reproducibility of Results
D006626:Hippurates; D003404:Creatinine; C030514:hippuric acid