title: Differences in tyrosine tRNA identity between Escherichia coli and archaeon, Aeropyrum pernix K1.
abstract: Recognition sites of tyrosine tRNA for tyrosyl-tRNA synthetase from Escherichia coli and extreme thermophilic archaeon, Aeropyrum pernix K1 were examined using various in vitro transcripts. With respect to the long variable arm in E. coli tyrosine tRNA, some base pairs in length was required for tyrosylation. None of the recognition sites were found in the acceptor stem, except the discriminator base A73 in E. coli tyrosine tRNA. In case of A. pernix tyrosine tRNA, C1-G72 base pair and discriminator base A73 in the acceptor region as well as anticodon were base specifically involved in tyrosylation by A. pernix tyrosyl-tRNA synthetase.
authors.affiliation: Department of Material and Biological Chemistry, Faculty of Science, Yamagata University, Yamagata 990-8560, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Iwaki
authors.forename: Haruichi
authors.initials: H
authors.lastname: Asahara
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Nagaoka
authors.forename: Junji
authors.initials: J
authors.lastname: Yokozawa
authors.forename: Takuya
authors.initials: T
authors.lastname: Umehara
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Kawarabayasi
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Koyama
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Sako
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kuno
authors.forename: Tsunemi
authors.initials: T
authors.lastname: Hasegawa
mesh_terms: D001105:Archaea / Q000235:genetics*; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics*; D009690:Nucleic Acid Conformation; D012365:RNA, Transfer, Tyr / Q000737:chemistry*
chemical_list: D012365:RNA, Transfer, Tyr