title: Characterisation of a new species of Pythium isolated from a wheat field in northern France and its antagonism towards Botrytis cinerea causing the grey mould disease of the grapevine.
abstract: A new species, Pythium bifurcatum, isolated from soil samples taken from a wheat field in Lille in northern France is described here. The oomycete occurred thrice out of 50 samples. The type specimen is F-91, which is a slow-growing saprophyte living on vegetable debris and which can be recognised by its antheridial as well as oogonial characteristics, which are different from other known species of Pythium. When grown together with Botrytis cinerea, the causal agent of the grey mould disease of the grapevine, Pythium bifurcatum shows a pronounced antagonism and suppresses its growth. Morphological features of this new species, its antagonism to B. cinerea, the sequences of the ITS region of its nuclear ribosomal DNA, and its comparison with related species are discussed in this article.
authors.affiliation: Laboratoire des Sciences de la Vigne, Institut Jules Guyot, Université de Bourgogne, BP 27877, 21078 Dijon, France. bernard.paul@u-bourgogne.fr
authors.forename: Bernard
authors.initials: B
authors.lastname: Paul
mesh_terms: D001483:Base Sequence; D020171:Botrytis / Q000254:growth & development*; D004275:DNA, Ribosomal / Q000235:genetics; D005602:France; D005800:Genes, Fungal; D025301:Hyphae; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000382:microbiology*; D011775:Pythium / Q000145:classification* / Q000235:genetics / Q000254:growth & development; D012988:Soil Microbiology; D013172:Spores, Fungal / Q000235:genetics; D014908:Triticum / Q000382:microbiology*; D027843:Vitis / Q000382:microbiology*
chemical_list: D004275:DNA, Ribosomal