title: An unnatural base pair between imidazolin-2-one and 2-amino-6-(2-thienyl)purine in replication and transcription.
abstract: Nucleosides of imidazolin-2-one (designated by z) were designed and synthesized as pairing partners of 2-amino-6-(2-thienyl)purine (designated by s). Previously, we developed an unnatural base pair between s and pyridine-2-one (designated by y), and polymerases specifically incorporated the substrate of y into DNA and RNA opposite s in templates. Although s was efficiently incorporated opposite y, A was also incorporated opposite y with high efficiency. The replacement of y by z effectively improved the incorporation selectivity of s. The incorporation efficiency of A opposite z decreased, but the efficient incorporation of s opposite z was maintained as compared to that of the s-y pairing.
authors.affiliation: Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Hirao
authors.forename: Michiko
authors.initials: M
authors.lastname: Kimoto
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Mitsui
authors.forename: Yoko
authors.initials: Y
authors.lastname: Harada
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Fujiwara
authors.forename: Akira
authors.initials: A
authors.lastname: Sato
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Yokoyama
mesh_terms: D020029:Base Pairing; D004261:DNA Replication; D007093:Imidazoles / Q000737:chemistry*; D011687:Purines / Q000737:chemistry*; D014158:Transcription, Genetic
chemical_list: C435686:2-amino-6-(2-thienyl)purine; D007093:Imidazoles; D011687:Purines