title: Unique and combinatorial functions of Fgf3 and Fgf8 during zebrafish forebrain development.
abstract: Complex spatiotemporal expression patterns of fgf3 and fgf8 within the developing zebrafish forebrain suggest their involvement in its regionalisation and early development. These factors have unique and combinatorial roles during development of more posterior brain regions, and here we report similar findings for the developing forebrain. We show that Fgf8 and Fgf3 regulate different aspects of telencephalic development, and that Fgf3 alone is required for the expression of several telencephalic markers. Within the diencephalon, Fgf3 and Fgf8 act synergistically to pattern the ventral thalamus, and are implicated in the regulation of optic stalk formation, whereas loss of Fgf3 alone results in defects in ZLI development. Forebrain commissure formation was abnormal in the absence of either Fgf3 or Fgf8; however, most severe defects were observed in the absence of both. Defects were observed in patterning of both the midline territory, within which the commissures normally form, and neuronal populations, whose axons comprise the commissures. Analysis of embryos treated with an FGFR inhibitor suggests that continuous FGF signalling is required from gastrulation stages for normal forebrain patterning, and identifies additional requirements for FGFR activity.
authors.affiliation: MRC Centre for Developmental Neurobiology, King's College London, London SE1 1UL, UK.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Walshe
authors.forename: Ivor
authors.initials: I
authors.lastname: Mason
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology; D015415:Biomarkers; D019521:Body Patterning; D016923:Cell Death; D002454:Cell Differentiation; D002455:Cell Division; D051518:Fibroblast Growth Factor 3; D051524:Fibroblast Growth Factor 8; D005346:Fibroblast Growth Factors / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D009024:Morphogenesis; D016376:Oligonucleotides, Antisense / Q000378:metabolism; D016548:Prosencephalon / Q000196:embryology* / Q000254:growth & development / Q000502:physiology; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D017468:Receptors, Fibroblast Growth Factor / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D015027:Zebrafish / Q000196:embryology*; D029961:Zebrafish Proteins
chemical_list: D015415:Biomarkers; D051518:Fibroblast Growth Factor 3; D016376:Oligonucleotides, Antisense; D011518:Proto-Oncogene Proteins; D017468:Receptors, Fibroblast Growth Factor; D029961:Zebrafish Proteins; C489200:fgf3 protein, zebrafish; D051524:Fibroblast Growth Factor 8; D005346:Fibroblast Growth Factors