[Differentiation and malignant suppression induced by mouse erythroid differentiation and denucleation factor on mouse erythroleukemia cells].
OBJECTIVE
To investigate the roles of mouse erythroid differentiation and denucleation factor (MEDDF), newly cloned in our laboratory, in erythroid terminal differentiation.


METHODS
Mouse erythroleukemia cells (MEL) were transfected with eukaryotic expression plasmid pcDNA-MEDDF. The changes of cell growth rate, mitotic index and colony-forming rate in semi-solid medium were investigated. The expressions of c-myc and beta-globin genes were analysed by semi-quantitative RT-PCR.


RESULTS
MEL cells transfected with pcDNA-MEDDF showed significant lower growth rate, mitotic index, and colony-forming rate in semisolid medium(P < 0.01). The percentage of benzidine-positive cells was 32.8% after transfection. The expression of beta-globin in cells transfected with pcDNA-MEDDF was 3.43 times higher than that of control (MEL transfected with blank vector, pcDNA3.1), and the expression of c-myc was decreased by 66.3%.


CONCLUSIONS
MEDDF can induce differentiation of MEL cell, and suppress its malignancy likely.
Zhao
Q
Q
Department of Cell Biology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China.
Ge
Y H
YH
Zhou
J P
JP
Ma
J
J
Chen
K Q
KQ
Xue
S P
SP
Han
D S
DS
D028341:Activins / Q000235:genetics / Q000502:physiology; D000818:Animals; D002454:Cell Differentiation; D015870:Gene Expression; D016259:Genes, myc / Q000235:genetics; D005914:Globins / Q000235:genetics; D028322:Inhibin-beta Subunits / Q000235:genetics / Q000502:physiology; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics / Q000473:pathology*; D051379:Mice; D014162:Transfection; D014407:Tumor Cells, Cultured
D028341:Activins; D005914:Globins; D028322:Inhibin-beta Subunits